LOCUS       DASRIG010000001         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_782498, whole genome shotgun sequence.
ACCESSION   DASRIG010000001 DASRIG010000000
VERSION     DASRIG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782498"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFB52_00005"
     CDS             <1..937
                     /locus_tag="VFB52_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZO04743.1"
                     /translation="CHLSSGQEATSVGTMAAMTPTDLLVTGYRCHGFALARGVPATEA
                     MAELFGRRDGCAGGRGGSMHLLDVERGFYGGWGIVAGQLPVATGLALALTRRGRQDAV
                     LCELGDGAVNMGAWHESLNLAALWNLPIVFLVVNNVYGMGTSVQRASAEPDLFKRASS
                     YRMASERVDGDDIEAVFDATDRLLRAAREERKPAVLEAMTYRYRGHSVADAGTAYRTK
                     DEIEERRGRDPIARVRSLLADRGAGEDALDAAEQRARALVAEAVEFADADPEPELDQL
                     ATGMYAPTSDLQFARMRPGSPFGEEELVYAAGLGA"
     gene            1009..1986
                     /locus_tag="VFB52_00010"
     CDS             1009..1986
                     /locus_tag="VFB52_00010"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO04744.1"
                     /translation="MNYREALRLAMREELARDEHVFLMGEEVGVFNGSYKVSDGLLEE
                     FGPDRIRDTPISEEGFVGAAVGAALLGERPIVEIMTLNFLLVAMDQVVNHAAKVGAMF
                     GGKVRCPLVIRTPNGAGNQLTAQHSQSFDGWFAGTPGLKVVAPSTPADAKGLLKAAIR
                     DDDPVLVIENLSAYKEKGEVPLDPDYLVPLGLAAVTREGTDISLVAHGFAAVRALEAA
                     ERLAEAGISAEVVDLRSLRPLDVETVAESVRKTNRAVLVEEGWPSFGVTAELAARIQH
                     VCFDYLDAPVERVGMAEVPLPYAKNLENAALPNVERVVAAARAVLDGKR"
     gene            1983..>2401
                     /locus_tag="VFB52_00015"
     CDS             1983..>2401
                     /locus_tag="VFB52_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZO04745.1"
                     /translation="MSEIVMPRLSDSMEEGTILTWMKQVGDEVAVGDELVEIETDKAN
                     MAYEADIAGTLTEILAQEGDTLPIGAPIARIGGEGEAAPAPPAEEPESEPEPTPEPTP
                     VSKPEKLEGERVKASPLARRLAKEKGIDLSAIAGSGP"
BASE COUNT          366 a          877 c          847 g          311 t
ORIGIN      
        1 ctgccacctc tcctcgggcc aggaggcgac ctcggtgggc acgatggcgg cgatgacgcc
       61 gaccgacctc ctggtcaccg gctaccgctg ccacggcttc gcgctcgccc gcggcgtccc
      121 cgcaacagag gcgatggccg agctgttcgg ccgccgcgac ggctgcgccg gcgggcgcgg
      181 cggctcgatg cacctgctcg acgtcgagcg cggcttctac ggcggctggg gcatcgtcgc
      241 cggccagctg ccggtggcga ccgggctggc cctggcgctg acccggcgcg gccgccagga
      301 cgcggtgctc tgcgagctcg gcgacggcgc ggtcaacatg ggcgcctggc acgagtcgct
      361 gaacctggcg gcactctgga acctgccgat cgtcttcctc gtcgtcaaca acgtctacgg
      421 gatggggacc agcgtccagc gcgcctccgc cgagcccgac ctgttcaagc gcgcctcctc
      481 ctaccggatg gcctccgagc gggtcgacgg cgacgacatc gaggccgtct tcgacgccac
      541 cgaccggctg ctgcgggcgg cgcgcgagga gcgcaagccg gcggtgctgg aggcgatgac
      601 ctaccgctac cgcggccact cggtcgccga cgccggcacc gcctaccgga ccaaggacga
      661 gatcgaggag cggcgcggcc gcgacccgat cgcgcgggtc cgttcgctgc tggcagaccg
      721 cggtgccggc gaggacgcgc tcgacgcggc cgagcaacgg gcgcgagccc tggtcgccga
      781 ggcggtcgag ttcgccgacg ccgatcccga gccggagctg gatcagctgg ccacggggat
      841 gtacgcgccg acttccgacc tgcagttcgc gcgcatgcgc cccggctcgc ccttcggcga
      901 ggaggagctt gtctacgcag cggggctggg cgcgtgaaca gccccggtcc agtcgccccc
      961 gccccgcccc gcgctgagga cgcggaacct tcggtcgcca gcgagcggat gaactatcgc
     1021 gaggcgctgc gcctggcgat gcgcgaggag ctcgcccgcg acgagcacgt cttcctgatg
     1081 ggcgaggagg tcggcgtctt caacggctcc tacaaggtca gcgacgggct gctcgaggag
     1141 ttcgggccgg accggatccg cgacaccccg atcagcgagg agggcttcgt cggcgccgcg
     1201 gtcggcgcgg cgctgctcgg cgagcgcccg atcgtcgaga tcatgaccct caacttcctg
     1261 ctggtggcga tggaccaggt cgtcaaccac gcggccaagg tcggcgcgat gttcggcggc
     1321 aaggtccgct gccccctcgt gatccgcacc cccaacggcg ccggcaacca gctcaccgcc
     1381 cagcactccc agagcttcga cggctggttc gccggcaccc ccggcctgaa ggtcgtcgcc
     1441 ccctccaccc cggccgacgc caagggcctg ctgaaggcgg cgatccgcga cgacgacccg
     1501 gtgctggtga tcgagaacct ctccgcctac aaggagaagg gcgaggtgcc gctcgacccc
     1561 gactacctgg tcccgctcgg gctcgccgcg gtcacccgcg agggcaccga catcagcctc
     1621 gtcgcccacg gcttcgccgc ggtccgcgcc ctcgaggccg ccgagcggct ggccgaggcc
     1681 gggatctccg ccgaggtcgt cgacctgcgc agcctgcgcc cgctcgacgt cgagacggtc
     1741 gccgagagcg tgcgcaagac caaccgcgcc gtcctggtcg aggagggctg gccgagcttc
     1801 ggggtcaccg ccgagctggc cgcgcgcatc cagcacgtct gcttcgacta cctcgacgcg
     1861 ccggtcgagc gggtcggcat ggcagaagtc cccctcccct acgcgaagaa cctcgagaac
     1921 gccgccctgc ccaacgtcga gcgcgtggtc gccgcggcgc gcgcggtcct agacggcaag
     1981 cgatgagcga gatcgtcatg ccccgcctct ccgactcgat ggaggagggg acgatcctga
     2041 cctggatgaa acaggtggga gatgaggtcg cggtcggcga cgagctggtc gaaatcgaga
     2101 ccgacaaggc gaacatggcc tacgaggcgg acatcgccgg gactctgacc gagatcctcg
     2161 cccaagaggg cgacaccctg ccgatcggag cgcccatagc tcggatcggg ggtgaaggtg
     2221 aagctgcgcc ggctccgcct gcagaggagc ccgagtcgga gccggagcca acccccgagc
     2281 ccactcccgt ctcgaaaccg gaaaagctcg agggcgagcg agttaaagcg tctcccctag
     2341 cccggcggct cgcgaaagag aagggcattg atctctcggc gatagctggc tcaggtccca
     2401 g
//
LOCUS       DASRIG010000002         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2607604, whole genome shotgun sequence.
ACCESSION   DASRIG010000002 DASRIG010000000
VERSION     DASRIG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607604"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFB52_00020"
     CDS             complement(<1..369)
                     /locus_tag="VFB52_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04746.1"
                     /translation="MPISRKLPMLAVLSAAALLLVLPAAAQAALTYVKGQLNRSVYVA
                     KDDGSNARKIGPGYSPHMAPDGATVAYFHEGPGHSTELKLAPVAGGPAKTLMVGWREP
                     FYLEFSPNGEQILALRGGELG"
     gene            complement(441..818)
                     /locus_tag="VFB52_00025"
     CDS             complement(441..818)
                     /locus_tag="VFB52_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04747.1"
                     /translation="MDWVVQVGLVELALGGLLGWAMVMRDEKPGWLKRIGVVAPHRIR
                     QVHLDYVMMGLILIAVGLAIRDLPSAIAIALVFGTFVNPFLFVPLAFDPEVEKQPWYR
                     ALAVVSFLAVSGGLVAAAIVGPA"
     gene            complement(828..1709)
                     /gene="thrB"
                     /locus_tag="VFB52_00030"
     CDS             complement(828..1709)
                     /gene="thrB"
                     /locus_tag="VFB52_00030"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZO04748.1"
                     /translation="MTARHRLVRVPASSANLGPGYDAMAAAVSLHLELEVEETGEYSF
                     DPGGMDVPTGRDNLIVRAFESLKPADGIAFRLRSEIPLARGLGSSAAAIVAGLFAADH
                     LFELALTRAEMLIKATEIEGHPDNVAAAIYGGFVVCGPGADGEPLASRFDPPGGLEGI
                     VVIPGEEVSTKLAREAIPAEVPLADAVANVSAAALLVLGLQSADLDLVSRGVADRIHQ
                     PRRRELYPRSMEIVDSATELGALGATISGAGPTVLVWTTWQEAGNVAAALEERCAGWA
                     EVRRLPFAPLGADVPEL"
     gene            complement(1706..2410)
                     /locus_tag="VFB52_00035"
     CDS             complement(1706..2410)
                     /locus_tag="VFB52_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04749.1"
                     /translation="MRRVRAHLTYANVMVTLLAFVVLGGVAYAATALPKNSVGTKQLK
                     KNAVTAAKIKAGAVNGAKVKDGSLTGADVNAATLGQVPSAQSAITAQTAQSAATAQNA
                     EQAENADRVGNFPPTAFAAAGRFRFESRPNGAVQQELYLLPGGVRVLTSGNSNFNIAF
                     DNTGSRTWRISTPTEQGPLGPGDEVVAFPAGFSALILIQDTADPDEAIALQCGRFEEP
                     ARIGCFATLSPELGPG"
     gene            complement(2410..>3308)
                     /gene="thrC"
                     /locus_tag="VFB52_00040"
     CDS             complement(2410..>3308)
                     /gene="thrC"
                     /locus_tag="VFB52_00040"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZO04750.1"
                     /translation="LKFEGLNPTGSFKDRGMTVAVSRAKGRGAEAVICASTGNTAASA
                     AAYAARAGLRGAVIVPEGKIAIGKLAQALMHGARVVALQGNFDDALRIVRELAHKHPI
                     ELVNSVNPHRIEGQKTAAFEVVEDLGGAPGALAIPVGNAGNVTAYWRGFQELETAPVL
                     YGFQAEGAAPLVAGEPVANPETVASAIRIGNPARWEEAMAAFTASRGRVAAVSDEQIL
                     DAYHWLASTEGVFCEPASASSVAGLLAHGLPVLEGTEPPESVVCVLTGHGLKDPDTAF
                     GKAPAVINCENDLSAVERAVFN"
BASE COUNT          465 a         1171 c         1203 g          469 t
ORIGIN      
        1 gccgagttcg cccccgcgca gggcgagtat ctgttcgccg ttcggcgaga actcgaggta
       61 gaacggttcg cgccagccga ccatcagcgt cttcgccggc cccccggcga ccggcgccag
      121 cttcagctcg gtgctgtgcc cgggaccttc gtggaagtag gcgaccgtgg cgccgtcggg
      181 cgccatgtgc gggctgtaac ccggcccgat cttgcgggcg ttgctgccgt catccttggc
      241 gacgtagacc gagcgattga gctggccttt cacgtaggtg agcgcggcct gtgcggcggc
      301 tgggagcacc agcagcagcg cggcggcgga gaggacggcg agcatgggga gcttccggga
      361 gatcggcatg gcgcgatcct acgtccgccc ggcggcggac cggacggatt tcgcagtccg
      421 gtcttggacg ccggccaagc tcaggccgga ccgacgatcg cggccgcaac caggccgccg
      481 ctgaccgcga ggaaggagac gaccgcgagc gcccggtacc agggctgctt ctcgacctcg
      541 ggatcgaagg cgagcgggac gaagaggaag gggttgacga aggtgccgaa gacgagtgcg
      601 atcgcgatcg cgctcggcag gtcgcggatc gccagcccga ccgcgatcag gatcaggccc
      661 atcatcacgt agtcgaggtg gacctggcgg atccggtgcg gcgcgaccac gccgatccgc
      721 ttcagccacc ccggcttctc gtcccgcatc accatcgccc agccgagcag ccctcccaag
      781 gcgagctcga ccaggcccac ctgcacgacc cagtccatgc gcggacccta aagctcgggc
      841 acgtcggcgc caagcggcgc gaacggcagc cgcctgacct cggcccagcc ggcgcagcgc
      901 tcctctagcg ccgcggcgac gtttccggcc tcctgccagg tggtccagac cagcacggtc
      961 gggccggcgc cggagatcgt cgcgcccagc gcgcccaact cggtcgccga gtcgacgatc
     1021 tccatcgagc gcgggtagag ctcgcgccgg cgcggctggt ggatgcggtc ggcgaccccg
     1081 cgcgagacca ggtcgaggtc ggcgctctgc agccccagca ccagcagcgc cgcggcggag
     1141 acgttggcga ccgcgtcggc gagcggcacc tcggccggga tcgcctcgcg cgccagcttc
     1201 gtcgagacct cctcgccggg gatcacgacg atcccctcga gcccgccggg cgggtcgaag
     1261 cggctggcca gcggctcgcc gtcggcgccg ggcccgcaga cgacgaagcc gccgtagatc
     1321 gccgcggcga cgttgtcggg atggccctcg atctcggtcg ccttgatcag catctccgcg
     1381 cgggtcagcg ccagctcgaa caggtggtcg gcagcgaaca gcccggcgac gattgccgcc
     1441 gcgctcgagc cgagcccacg cgccagcggg atctcgctgc gcagccgaaa ggcgatcccg
     1501 tccgccggtt tgaggctctc gaacgcccgc acgatcaagt tgtcacgccc ggtcggcacg
     1561 tccatcccac ccgggtcgaa cgagtactcc cccgtctctt ccacctccag ctcgaggtgc
     1621 agcgagaccg ccgccgccat cgcgtcatag cccggcccga ggttggccga ggaggcgggg
     1681 acgcggacga gtcggtggcg cgcggtcatc cggggccgag ctcgggagag agcgtcgcga
     1741 agcagccgat ccgggccggt tcctcgaagc gcccgcactg cagcgcgatc gcttcgtcgg
     1801 ggtcggccgt gtcctggatc aggatcagtg ccgagaaacc ggcagggaag gcgaccactt
     1861 cgtcaccggg ccccagcggc ccttgttcgg tcggcgtcga gatccgccag gtccggctgc
     1921 cggtgttgtc gaaggcgatg ttgaagttcg agttccccga ggtgaggacg cggacgcctc
     1981 ccggcagcag gtagagctcc tgctgaacgg ccccgttcgg ccgcgactcg aagcggaagc
     2041 gacccgccgc ggcaaacgcg gtcggcggga agttgccgac ccggtcggcg ttctcggcct
     2101 gctcggcgtt ctgcgcagtc gcggcgctct gcgcggtctg cgccgtgatc gcgctctgcg
     2161 ccgagggcac ctgaccgagg gtcgccgcgt tgacgtcggc gccggtcagc gagccgtcct
     2221 tgaccttggc cccgttcacc gcccccgcct tgatcttcgc cgcggtcacc gcgttcttct
     2281 tcagttgctt cgtgccgacg ctgttcttgg gcaacgccgt cgcggcgtag gcgaccccgc
     2341 cgagcactac gaaggcgagc agggtcacca tcacgttggc gtaggtcagg tgggcgcgga
     2401 cccgccgcat cagttgaaga ccgcgcgctc gaccgcggac aggtcgttct cgcagttgat
     2461 caccgcgggg gctttgccga aggcggtgtc ggggtccttg aggccgtggc cggtgagcac
     2521 gcagacgacc gactcgggcg gctcggtgcc ctcgagcacg ggcaggccgt gggcgagcag
     2581 gccggcgacc gaggaggccg aggcgggctc gcagaagacg ccttcggtcg aggcgagcca
     2641 gtggtaggcg tcgaggatct gctcgtcgga gacggcggcg acgcggccgc gcgaggcggt
     2701 gaaggcggcc atcgcctcct cccagcgggc cgggttgccg atccggatcg ccgaagcgac
     2761 ggtctccggg ttggcgacgg gctcgccggc gaccagcggc gccgcgccct cggcctggaa
     2821 gccgtagagg accggcgccg tctccagctc ctggaagccg cgccagtagg ccgtcacgtt
     2881 gccggcgttg ccgaccggga tcgcgagcgc gccgggggcg ccgccgagat cctcgacgac
     2941 ctcgaaggcc gccgtcttct ggccttcgat ccggtgcggg ttgaccgagt tgacgagttc
     3001 gatcgggtgc ttgtgggcga gctcgcggac gatccgcaga gcgtcgtcga agttgccctg
     3061 cagggcgacc acgcgggcgc cgtgcatcag cgcctgggcg agctttccga tcgcgatctt
     3121 cccctccggg acgatcaccg ctccgcgcag gccggcccgc gccgcgtagg cggcggcgct
     3181 cgctgcggtg ttgccggtcg aggcgcagat caccgcctcc gccccgcgcc ccttggcgcg
     3241 tgagaccgcg acggtcatcc cccggtcctt gaacgagccg gtcgggttga ggccctcgaa
     3301 cttgaggt
//
LOCUS       DASRIG010000003         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3652802, whole genome shotgun sequence.
ACCESSION   DASRIG010000003 DASRIG010000000
VERSION     DASRIG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3652802"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1736
                     /locus_tag="VFB52_00045"
     CDS             339..1736
                     /locus_tag="VFB52_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZO04751.1"
                     /translation="MTRRSLVPALRWIAAAALPVLLLALYLTYSRGGVAILVVACGTL
                     AILSHDRLWMLGTLAAGALGALPAVLAVQENRYLADNIDAPQAVDQGLTVLLVLVAGI
                     ALTVVLFAAIRRLAESRGELASRAVALSRDSRVLKWIAVAMALLAIGAALAVGGRAWD
                     QFTTSDLTTATPEGRFNDLSGGGRGEFWRVAIDAFAEDPVGGHGAGTYKFSWDELRDI
                     PKPVTEAHSLYLESFAELGLIGGLLVLGMVGAMIWFGFSAWRAARGSQRELYAALLAA
                     VVGFAVGAAYDWLWEVASVGFIFFLAGGTLIAARCAQLVAARASTNGQGDRRRFGLAV
                     AGLALAWVTALALVGPLLVDRELDASRAAAADGNFAQAVSKAETARSIEPFAASPYLQ
                     LGLLAQAQGDFVTARERLQQAIDREDRNWILYYVLARMEDEAGDAEAATAALEEARRL
                     NPEEACLDEGFEGCG"
     gene            1733..3214
                     /locus_tag="VFB52_00050"
     CDS             1733..3214
                     /locus_tag="VFB52_00050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO04752.1"
                     /translation="MSRTQDKQKARSTAAASPARQPAEPPADVRFAGAEGSRRRGALL
                     RRLLALGDWTALIGTLFVVTATTSTTDVGTLFWALLFSPLWILVIKLHGLYDNDHRRI
                     RHSTLDELPSLVSASVLGTLVLDGLLALSPVGPLSPTSAIALGLGTLVVSFILRGILR
                     FFWHRLTGLAFGLVIGAAPTVDMVARRVSTHPETRLALVGYLSVEDDEEATELPRLGS
                     LGDISEVAREYAIERVVVTELEMSEPAAEQLIEECKAAGLGLTFLPQHYGLLGPGIEL
                     NRLAELPVLDFRFSDPPRSTIAMKRAMDVVVSGTLLVLLSPVLLATALAILLDTGKPV
                     FFRQRRAGKEGEPFTMLKFRTMGTDAEERLGELVDLAKLDEPAFKIPDDPRVTRSGRF
                     LRRTSIDELPQLINVLRGDMSLVGPRPEEESVVALYDERQRKRLAIKPGLTGPMQVYG
                     RSDLTFEERLAMERDYLDNLSLITDLQILLRTPRAIVRGDGAY"
BASE COUNT          422 a         1174 c         1146 g          510 t
ORIGIN      
        1 gactggtgct ggcccttgcg cttttctctg gattctcatc tctctggtcc gggtcggttg
       61 agttgagcgt caccgaggca gatcgcgtcc tcgtctacct cggcgtcctc cttgccgcgt
      121 tcctgatcgc ccaaaccgac caaaggcgcc aacgtttcgc tgaagggatc gcagtcgggc
      181 tgatcggaat cgcgattttg ggagtagcga cccgtctgct gccccacgtt ttccccgtcg
      241 cagagggcct gggcagcggt ccgcggctgc gctacccgct cgattactgg aacgcgaacg
      301 cgaccgcctg tgcaatcggc gctgcaatgg ccgtttggat gacgcggcgc tccctggtcc
      361 cggccctgcg ctggatcgcg gcggcggcgc tgccggtgct gctgctggcg ctctacctga
      421 cctactcgcg cggcggcgtg gcgatcctcg tggtcgcctg cggcacgctc gcgatcctct
      481 cccacgaccg cctctggatg ctcggcacgc tcgcggccgg ggcgctgggg gcgctgccgg
      541 cggtgctcgc ggtgcaggag aaccgctacc tcgccgacaa catcgacgcc ccgcaggcgg
      601 tcgaccaggg cctcaccgtg ctgctcgtcc tggtcgccgg gatcgcgctg accgtggtct
      661 tgttcgcggc gatccggcgg ctcgccgaga gccgcgggga gctggccagc cgggcggtgg
      721 cgctctcccg cgacagcagg gtgctgaagt ggatcgcggt ggcgatggcg ctgctggcga
      781 tcggcgcggc gctggcggtc ggcgggcgcg cctgggacca gttcacgacc tcggacctga
      841 ccacggcgac cccggagggc cgtttcaacg acctctcggg cggcggccgc ggcgaattct
      901 ggcgggtcgc gatcgatgcc ttcgccgaag acccggtcgg cggtcacggc gccggtacct
      961 acaagttctc ctgggacgag ctgcgcgaca tccccaaacc ggtgaccgag gcgcactcgc
     1021 tgtacctgga gtcgttcgcc gagctcggcc tgatcggcgg gctgttggtg ctgggaatgg
     1081 ttggggcgat gatctggttc gggttctcgg cctggcgcgc ggcgcgaggg agccaacgcg
     1141 agctctacgc ggcgctgctc gccgctgtcg tcggcttcgc ggtcggcgcc gcctacgact
     1201 ggctctggga ggttgccagc gtcggcttca tcttcttcct cgccggcggc accctgatcg
     1261 cggcccgctg cgcgcaactg gtcgccgccc gcgccagcac caacggccag ggggatcggc
     1321 gccgcttcgg gctggccgtc gccgggctgg ccctggcctg ggtcacggcg ctcgccctgg
     1381 tcgggccgct gctggtcgac cgcgagctcg acgcgagccg cgcggccgcc gccgacggca
     1441 acttcgcgca ggcggtctcg aaggcggaga ccgcgcgctc gatcgagccc ttcgccgcct
     1501 ccccctacct gcagctcggc ctgctcgcgc aggcgcaggg cgacttcgtg accgcgcgcg
     1561 agcggcttca gcaggcgatc gaccgcgaag accgcaactg gatcctctac tacgtgctgg
     1621 cacggatgga ggacgaagcc ggcgacgcgg aggcggcgac ggcggcgctc gaagaagcgc
     1681 gtcgcctcaa tcccgaagaa gcgtgcctgg acgaaggctt cgaaggctgc ggatgagtcg
     1741 cacgcaagac aagcagaagg cgcgcagcac agcggcggcg agcccggcgc ggcagccggc
     1801 cgagccgccc gcagacgtca ggttcgccgg cgccgagggc tcgcggcggc gcggcgcgct
     1861 gctgcgccgg ctgctcgcgc tgggcgactg gacggcgctg atcggaacgc tcttcgtggt
     1921 caccgcgacg acctcgacca ccgacgtcgg gacgctgttc tgggcgctgc tgttcagccc
     1981 gctctggatc ctcgtgatca agctccacgg cctctacgac aacgaccacc ggcggatccg
     2041 ccacagcacg ctcgacgagc tgccctcgct ggtctcggcc agcgtgctgg gcacgctggt
     2101 cctcgacggg ctgctggcgc tgagcccggt cggcccgctc tcgccgacca gcgcgatcgc
     2161 gctcgggctc ggcaccctgg tcgtcagctt catcctgcgc gggatcctgc gtttcttctg
     2221 gcaccggctc accggcctcg ccttcgggct cgtgattggc gcggcaccga cggtcgacat
     2281 ggtcgcgcgc cgcgtctcca cgcacccgga gacgcgtctg gcgctggtcg gctacctctc
     2341 cgtcgaggac gacgaggagg cgacggagct gccgcgcctg ggctcgctcg gcgacatctc
     2401 cgaggttgcc cgcgagtacg cgatcgagcg cgtcgtcgtc accgaactgg agatgagcga
     2461 gccggccgcc gagcagctga tcgaggagtg caaggcggcc ggtctcgggc tcaccttcct
     2521 gccccagcac tacggcctgc tcgggcccgg gatcgagctc aaccgcctgg ccgagctgcc
     2581 ggtcctcgac ttccgcttca gcgacccacc gcgctcgacg atcgcgatga agcgggcgat
     2641 ggacgtggtc gtctccggca ccctgctggt cctgctctcc ccggtgctgc tggcgaccgc
     2701 gctggcgatc ctgctcgaca ccggcaaacc ggtcttcttc cgccagcgcc gcgccggcaa
     2761 ggagggcgag ccgttcacga tgctgaagtt ccgcacgatg gggaccgacg ccgaggagcg
     2821 gctcggcgag ctggtcgacc tggcgaagct cgacgagccg gccttcaaga tccccgacga
     2881 cccgcgggtc acccgcagcg gccgcttcct gcgccggacc agcatcgacg agctgccgca
     2941 gctgatcaac gtcctgcgcg gcgacatgtc gctggtcggg ccgcggccgg aggaggagag
     3001 cgtggtcgcc ctctacgacg agcgccagcg caagcgcctc gcgatcaagc cggggctgac
     3061 cgggccgatg caggtctacg gccgcagcga cctgaccttc gaggagcggc tggcgatgga
     3121 gcgcgactac ctcgacaacc tctcgctgat caccgacctg cagatcctcc tgcgcacccc
     3181 ccgcgcgatc gtccgcgggg acggcgccta ctagcgtccg cgggactttg agcaccccgg
     3241 cccccgacct cg
//
LOCUS       DASRIG010000004         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2348273, whole genome shotgun sequence.
ACCESSION   DASRIG010000004 DASRIG010000000
VERSION     DASRIG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348273"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFB52_00055"
     CDS             <1..683
                     /locus_tag="VFB52_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZO04753.1"
                     /translation="AGHEESGSQPSSEGNGDEGLEHLLDAACRTISRRGFSATRVADI
                     AAEAGVSPATVHYHFKTRHEILVDALLWANARLVKELERANRNGDPPLVRIARFLERT
                     VPHPGAREDEYRLEIDIWGQARHHPELLGPYEGFAARWTNELAEMIEDGVAAGELAPT
                     VETPELVERLVALTDGLAARAVTGSAAMPPDRVHVLLFRFAAEQLGIEPEELERHAEL
                     PPLRSYGE"
     gene            complement(688..1032)
                     /locus_tag="VFB52_00060"
     CDS             complement(688..1032)
                     /locus_tag="VFB52_00060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZO04754.1"
                     /translation="MSELPLSQSRTAGGLVFVSGQVGLESDGTVPGSFERQAELALGA
                     LLAALREADAGAVMKTTLYLVRREDFAAMNEVYSRYFSAPWPARTTLVAELALPGLLF
                     EVDAVARTASGT"
     gene            complement(1025..>2825)
                     /locus_tag="VFB52_00065"
     CDS             complement(1025..>2825)
                     /locus_tag="VFB52_00065"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HZO04755.1"
                     /translation="APIAAVRHRDELAGVEAELATTDGVTVLIYDDRCATEERRLRKR
                     GQLPAPSKRVWINERVCEGCGDCGEKSTCLSVQPVETELGRKTRIHQASCNQDYSCLD
                     GDCPSFLVVEPGRREAPDRPAPPAELPAPPAPPAGEVLVRMPGIGGTGVVTVSQILQM
                     AAHLDGIEATGLEQIGLAQKGGPVTSDVRFAADGIRGGLRASRGRADAIIGFDLLGAA
                     SEANLEVADPARTVAIVNTGETPTAQMVTDVAATAAEAEQMRRRIDAATRSEDNIYLD
                     AEGISLGLFGDHMPANMLLIGVAFQAGCLPLSAAAIERAIELNGAAVEVNIEAFRWGR
                     AAVALPAELARVAPSPLPAPEPDQALAALLAEREMPAALRGLLARRGTDLVGYQSRRY
                     ACEYVEDVLGVATAIEAAIGAGAEPLALAYARNLHKLMAYKDEYEVARLHLDELEAAR
                     LRAELGEGAKVKVMLHPPLLRAMGMKRKLAFGRTARPLLGLLRRGKFLRGRALDPFGR
                     AHVRRIERELIGEYRELVATALAELTPECAHLFAELIDLPDRIRGYEGIKLASVERFR
                     VNAQRLNDLIVKAPGEEVSVGEQQGSERTGNRV"
BASE COUNT          417 a          998 c          996 g          414 t
ORIGIN      
        1 gcgccgggca cgaggagagc gggtcccagc cttcgtccga ggggaatggc gacgagggcc
       61 tcgaacacct gctcgatgcc gcctgtcgca cgattagcag gcgtggattc agcgcgaccc
      121 gagtagccga catcgccgcc gaggccgggg tctctccagc caccgtccac taccacttca
      181 agacccgcca cgagattctc gtcgatgcgc tgctctgggc caacgcccgc ttggtgaagg
      241 agctcgagcg cgccaatcgc aacggcgacc ctcccctggt ccgcattgcc cggttccttg
      301 agcgcaccgt ccctcacccc ggcgcgcgcg aggacgagta tcggctcgag atcgacatct
      361 gggggcaggc gcgccatcat ccggagctgc tcgggcccta cgagggcttc gcggcgcgct
      421 ggaccaacga gctggcggag atgatcgagg acggtgtcgc cgcgggagaa ctcgcaccga
      481 ccgtggagac gccggagttg gtcgagcgcc tcgtcgcact taccgacggg ctcgctgccc
      541 gggcagtgac cggctccgcc gcgatgcctc cggatcgggt gcacgttctt ctcttccggt
      601 tcgccgctga gcagctcggc atcgagcccg aggagctcga gcgccacgcc gagctgccgc
      661 cgctgcgctc gtacggcgag taacacctca agtccccgaa gcggtgcggg cgaccgcatc
      721 gacctcgaac aacagccccg ggagggcgag ctcggcgacc agcgtggtcc gggccggcca
      781 gggcgccgag aagtagcgtg agtagacctc attcatcgcc gcgaagtcct cgcggcggac
      841 caggtaaagc gtggtcttca taaccgcgcc ggcgtcggcc tcgcgtagcg ccgccagcag
      901 cgcccccagc gccagctcgg cctgacgttc gaaggagccc gggaccgtac cgtcggactc
      961 gagcccgacc tgaccggaga cgaacaccag gccgccggcg gtgcggctct ggctcagcgg
     1021 cagctcagac acgattcccg gtccgctcgc tgccctgctg ctctcccacc gacacttcct
     1081 cgccgggcgc cttgacgatc agatcgttga gcctctgcgc attgacccgg aaacgctcga
     1141 cgctggcaag cttgattccc tcgtagccgc ggatccggtc gggcaggtcg atgagctcgg
     1201 cgaagagatg ggcgcactcg ggggtcagct cggcgagcgc cgtggcgacg agttcgcggt
     1261 actcgccgat cagctcgcgc tcgatccggc ggacatgggc gcggccgaag ggatcgaggg
     1321 ccctcccgcg caggaacttc ccgcggcgca ggagcccgag cagcggccgc gcggtgcgcc
     1381 cgaaggcgag ctttcgcttc atccccatcg cccgcaaaag cggcgggtga agcatgacct
     1441 tgaccttggc gccctcgccc agttcggccc gcaggcgcgc cgcctcgagc tcgtcgagat
     1501 gcaggcgtgc cacctcgtac tcatccttgt aggccatcag cttgtgcagg ttgcgggcgt
     1561 acgcgagggc cagcggctcg gcgccggcac cgatcgccgc ctcgatcgcg gtggcgaccc
     1621 cgagcacgtc ctcgacgtac tcgcaggcgt aacggcgact ctggtagccg acgaggtcgg
     1681 tgccacgccg agcaagcaga ccgcgcagcg ccgctggcat ctcgcgctcg gcgagcaggg
     1741 cggcgagtgc ctgatcgggc tcgggggcag gcaggggcga cggcgcgacc cgagccagct
     1801 cagcgggcag ggcgaccgcc gcccggcccc agcggaaggc ctcgatgttt acctcgacgg
     1861 cggcgccgtt gagctctatc gcgcgctcga tcgctgccgc cgacagcggc agacagccgg
     1921 cctggaacgc gacgccgatc agcagcatgt tggccggcat gtggtcgccg aagagaccca
     1981 aggagatgcc ctcggcgtcg aggtagatgt tgtcctcgct gcgcgtcgcg gcgtcgatcc
     2041 gccgccgcat ctgctctgcc tcggcggcgg tcgcggcgac gtcggtgacc atctgcgcgg
     2101 tcggggtctc gccggtgttg acgatcgcca cagtgcgggc ggggtcggcg acctctaggt
     2161 tggcctcgct ggcggcgccg agtaggtcga agccgatgat cgcgtcggcg cgcccgcggc
     2221 tggcgcgcag gccgccgcgg atcccgtccg cggcgaagcg aacgtcggag gtaaccggac
     2281 ctcccttctg ggccagcccg atttgctcca ggccggtcgc ctcgattccg tctaaatggg
     2341 ccgccatttg caggatctgg gagacggtga caacgccggt gccgccgatt cccggcattc
     2401 gcacgagcac ctcgccggcc ggcggcgcag gcggggccgg cagctcggcc ggcggcgcgg
     2461 ggcggtcggg ggcctcgcga cggcccggct cgacgacgag gaatgacgga cagtcgccgt
     2521 ccaggcaaga gtagtcctgg ttgcaggagg cctggtggat tcgggtcttc cgccccagct
     2581 cggtctctac cggctgcacc gagaggcagg tcgacttctc gccgcaatcg ccgcagccct
     2641 cgcagacgcg ctcgttgatc cagacccgtt tgctcggcgc gggcagctga ccgcgcttgc
     2701 gcaggcggcg ctcctcagtg gcgcaacgat cgtcgtagat cagcaccgtg acgccatcgg
     2761 tcgtggccag ttcggcttcg accccggcga gctcgtcgcg gtggcggacg gcagctatcg
     2821 gtgcc
//
LOCUS       DASRIG010000005         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1047780, whole genome shotgun sequence.
ACCESSION   DASRIG010000005 DASRIG010000000
VERSION     DASRIG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047780"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..642)
                     /locus_tag="VFB52_00070"
     CDS             complement(349..642)
                     /locus_tag="VFB52_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04756.1"
                     /translation="MFFAAPAKFLVVLRPGNGTPNTFLCRLRVSAMPPASPARLAAAT
                     NAGVLSFLAVDPTASPALVATLTDASLAFSAVPLLLEERDELDCLRAVDPALL"
     gene            complement(852..2321)
                     /locus_tag="VFB52_00075"
     CDS             complement(852..2321)
                     /locus_tag="VFB52_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO04757.1"
                     /translation="MTLEGNRRWLALYVLCLGDVLIVLDSTIVNVALPSIQGDLGFSD
                     ATLAWVVNAYLLTFGGFLLLGGRLSDLYGRRRLFLIGLGLFTTSSLACGLAGSQEFLV
                     GARAVQGFGGAIVSVVALSLIMTIFTETAERAKAMGVFSFVLSGGATAGVLLGGVLTD
                     LLAWNWIFLVNLPVGITVFVLSLRLLPDDRPEAGDRRLDVGGAVTVTAAVMLGTYAVI
                     NGNENGWTSGETIGLLAAATTLLAFFLVLESRVGSPLMPLGLFRLRNISVANVVGVLL
                     AGAMFGWFFLSALYLQLVLGYTPLEVGLAYLPSTLIMAAGSIALSDRLVMRFGVKPPI
                     VGGLSCFAIGLLWFARAPADGSFVVDVLPAMLVMGIGGGITFNPLLLAAMGDVAPTES
                     GLASGIVNTAFMMGGALGLAVLVSVADSRTQSLLDSGDGHAAALTGGYNAAFLVAAAF
                     AVIGALGALLLRPAAPPGHGEMPEEARAVAEPVPESSPV"
     gene            complement(2392..3999)
                     /locus_tag="VFB52_00080"
     CDS             complement(2392..3999)
                     /locus_tag="VFB52_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO04758.1"
                     /translation="MLLEGAQLEAPAAPLDDLLRIGLASAPDEVAITSAQRALSWREL
                     DRASAALAGGYRGRGLEPGDRLASLMPNRVDLVVHYLACFRAGLIVTPLNYRYTAREI
                     DHALEVSGARALLAHVERAEDVGASELAAALELGTIAYRDAEPLATGNEEPAGVDGGW
                     RHDFAALLGSEPLAGTETEPEPEAPAVIFFTSGSTGPAKGVTHTRETLRWMIGAASAA
                     FELDASDVLLPGSSMSHIGSFLWALSTLSVGGKVVVARSTDGHELLPLLREQQPTILA
                     MIPAALSALVHDHGLRPGDFSSLRLCRAGADKVSTELLTEFAATAGFPIVEGYGMSEV
                     GLATMNPPSGEIRQGSIGRPIHGFTIALRDQQGAPTEGGDVGKIWIRSRTRMLGYWGA
                     PSATEEVVIGGWLDSGDLARADEDGYLWFFGRKKQVIVHDGSNISPYEVEGALVEHPA
                     VSLAGAVGVHDAVHGENVRAYVTLRNDVEPPSRADLIVFCRERIGYKAPDEIVFLDEM
                     PLNPTGKIDRVGLKRMAEDHLHPHGLS"
     gene            4445..>4719
                     /locus_tag="VFB52_00085"
     CDS             4445..>4719
                     /locus_tag="VFB52_00085"
                     /inference="COORDINATES: protein motif:HMM:NF027966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HZO04759.1"
                     /translation="MIWTRVTSVAEAVDVSWVVATDHELAEVVAGGAAVASAQDDHTV
                     QVHVGGLEPGREYFYGFAVAAEHSQTGRTRTLAAEPERVRFATCSCA"
BASE COUNT          878 a         1572 c         1617 g          652 t
ORIGIN      
        1 cttgtactcc tgaaggcggg agaggtaata gctgctgggc tcggccatcg caaatcttcg
       61 accaataccc gcaaattccg actatcggct tcggtccggg ggtaatctag ctcgacagct
      121 cttaaggagg ggttcagtgg cacagcaggc gaacggcaga agcacccgaa gatccagtgc
      181 aggcacctcg aagccgcgca gctcgtcgac gaatacgcgg agcaccaagt cgcgatccag
      241 ctcaagccgc tcacagggca gccgcacggc gcagagcaag ggcggatcga cggcgcggag
      301 gccgtcgagc tcatcgcgct caagccgctc acagggcagc cgcacgtctc agagcaaggc
      361 cggatcgacg gcgcggaggc agtcgagctc atcgcgctct tcgagcaaca aaggtacggc
      421 cgagaaggcc aaggacgcgt cggtcaaggt cgcaaccagc gccggagacg cggtggggtc
      481 gaccgccaag aagctgagga ctccagcgtt ggtcgcagca gccagcctcg ccgggctcgc
      541 cggtggcatc gcgctgacgc gcagccgaca aaggaaggtg ttgggcgttc cgttgcccgg
      601 gcgcagtacc accaagaatt tcgccggcgc tgcgaagaac atcggtgcgc tcgccgagcg
      661 caccggtcgt gtcgccgagc aggtacgagt cgctagcgag gcgctcgggg atcacgacgc
      721 tcgccgatcc tctaacgatg ctcgccgatc tccgatcgag gtcgtcctcg agggcctaac
      781 gagacggtcc caagcagcaa agtccaactg acgcgcgcca gctgaccgcg cgagcgaagc
      841 ccagcggggc gtcagacggg cgacgattcg ggcaccggct ccgcgaccgc gcgcgcttcc
      901 tcgggcatct ccccgtgtcc gggtggtgcg gcgggacgca ggagcagcgc gccgagcgcg
      961 ccgatcactg cgaacgcggc ggcgaccagg aatgccgcgt tgtagccgcc ggtcagcgca
     1021 gccgcgtgac cgtcgccgga gtcgaggagg ctctgtgtgc gcgagtccgc cacgctgacg
     1081 agcaccgcca ggcctagagc gccgcccatc atgaacgccg tgttgacgat ccccgaggcc
     1141 agcccgctct cggtcggcgc gacgtcgccc atggcagcga gcagcagtgg gttgaaggtg
     1201 atcccgccgc cgatccccat aacgagcatg gccgggagca cgtcgacgac gaagctgccg
     1261 tcggcgggcg cccgcgcgaa ccaaagcagg ccgatggcaa aacacgagag gccgccgacg
     1321 atcggtggct tcacgccgaa gcgcatcacg agccggtcgg agagcgcgat cgaacctgcg
     1381 gccatgatca gcgtcgacgg caggtaggcg aggccaacct caagaggggt gtagccgagc
     1441 acgagctgga ggtagagcgc cgaaagaaag aaccagccga acatcgctcc cgccaagagg
     1501 acgccgacga cattggcgac cgagatgttg cgcaggcgaa acaggccgag cggcatcagc
     1561 ggcgagccga ccctcgactc gagcacgagg aagaaggcga gcagggttgt ggccgcggcg
     1621 agcagcccaa tggtctcgcc ggaggtccaa ccgttctcgt tgccgttgat gaccgcgtag
     1681 gtgccgagca tcacggcggc ggtcacggtc acggccccgc cgacgtcgag gcgccgatca
     1741 cctgcttccg ggcgatcatc gggcaacagc ctcaagctca gcacgaagac cgtgatgccc
     1801 accgggagat tcaccaagaa gatccagttc caggcgagca gatcggtgag cacgccgcct
     1861 aagagcaccc cagccgttgc gccgccggac agcacgaagc tgaagacacc catcgccttg
     1921 gcgcgctcgg cggtctcggt gaagatggtc atgatgagcg agagcgcgac cacggacaca
     1981 atcgctccgc cgaagccctg cacagcacgt gcgccgacca ggaactcctg cgacccagcc
     2041 aaaccacagg cgagcgagga ggtggtgaag agccccaggc cgatcaggaa cagccgacgg
     2101 cggccataga gatcgctgag gcgcccgccg agcaacagga agcccccgaa ggtgagcagg
     2161 taggcgttca ccacccaggc gagcgtcgcg tcggaaaagc cgagatcgcc ctgaatcgac
     2221 ggcagggcga cgttgacgat cgtcgagtcg aggacgatca gcacgtcacc gagacagagc
     2281 acatagagcg cgagccatct cctgttgccc tcgagggtca tcaaggcgat gaaactagcg
     2341 attcgactgc gtttgaagtc agtgcttgct cgccggagac ggccgtttcg atcagctcag
     2401 accgtggggg tgaaggtggt cctcggccat acgcttgagg ccgacccgat cgatcttgcc
     2461 ggtcggattg aggggcatct cgtcgaggaa gacaatctcg tccggcgcct tgtagccgat
     2521 gcgctcgcgg cagaagacga tcaggtcggc gcgcgacggc ggctcgacgt cattgcgcag
     2581 cgtcacgtag gcgcgaacgt tctcgccgtg gaccgcgtcg tggacgccga cggcaccggc
     2641 cagcgagacc gcggggtgct cgaccagagc gccttcgacc tcgtacgggc tgatgttgga
     2701 gccgtcgtgc acgatcacct gcttcttgcg gccgaagaac cagaggtagc cgtcctcgtc
     2761 tgcccgggcg agatcgcccg agtccagcca gccgccgatc acaacctcct cggttgcgct
     2821 gggcgccccc cagtagccga gcatgcgggt gcggctgcgg atccagatct tgcccacgtc
     2881 tccgccctcg gtgggtgcgc cctgctgatc tcgcagcgcg atggtgaagc catggatcgg
     2941 cctcccgatc gagccttgcc tgatctcacc cgagggcgga ttcatcgtcg ccaacccgac
     3001 ctcgctcatc ccgtatccct cgacgatcgg gaagcctgcc gttgcggcga actcggtcag
     3061 cagctcggtc gagaccttgt cggcgccggc ccggcacagc cgcagcgagg agaagtctcc
     3121 gggccgcaga ccgtggtcgt gaacgagtgc cgacagcgcc gccgggatca tcgcgaggat
     3181 cgtcggctgc tgctcgcgca acagcggcag caactcgtgg ccgtccgtgc tgcgcgccac
     3241 cacgaccttg ccaccgaccg agagcgtcga aagcgcccac agaaaggagc cgatgtggga
     3301 catcgaggag ccgggcagaa gaacgtcaga ggcatcgagc tcgaacgctg cggaggcagc
     3361 gccgatcatc cagcgcaggg tctcgcgggt atgggtgacc cctttggcgg ggccggtgct
     3421 ccccgaggtg aagaagatca cggccggcgc ctcgggttcg ggctccgtct ccgtgcctgc
     3481 aaggggctcg gagccgagga gcgcggcgaa gtcgtggcgc caaccgccgt cgaccccggc
     3541 cggctcctcg ttgccggttg cgagcggttc cgcatcgcgg tacgcgatcg ttccaagctc
     3601 cagggcggcg gcaagctcgc tggcgccgac gtcctcggcg cgctcgacat gggcgagaag
     3661 agcgcgggcg ccgctcacct ccagcgcgtg atcgatctcg cgggcggtat agcggtagtt
     3721 cagcggggtc acgatcagtc ccgccctgaa gcaggcgagg tagtggacga cgaggtcgac
     3781 ccggttcggc atcagggacg cgaggcgatc gcccggctcg aggccacgcc cgcggtatcc
     3841 accggccagc gcggcgctcg cccggtcgag ctcgcgccaa ctgagcgcgc gctgggccga
     3901 agtgattgcg acttcgtccg gggcggacgc gagaccgatc cgcagcagat catcaagcgg
     3961 cgcggcaggg gcttcgagct gggcaccctc gagcagcatc cgctcgatcc tacgcgagta
     4021 ccgccgtcct tctgaaggcc gacgacatcg cgatcgccgc cctcgactgc ggaccctcgc
     4081 ggactccgcg gccgccgccg agacggacga ggaggtcgct acaccccggc gccaggcagt
     4141 ccttcgcggc atgtggcgtg gatagccgcg acaaacgggc ggtctctgtc ctggatctcc
     4201 gcgcggagct caacgaacgg cctcaatgac tgatgatcct cgccgcgctg gaccatccag
     4261 acaccccaag cattgtgcac gtcttccgca gtggtgtgga ttgccttgat gaggatcccg
     4321 ccaggtggca gacggctgaa agacttcggc caatggtcaa gccaaggcgc atggtgtccg
     4381 gctgatgggc gccggggtat ttcgtcacgg ggtggcaagt ggcgatcccc tgacggaccg
     4441 ggtcatgatt tggacccggg tcacgagtgt ggccgaggcc gtcgacgtca gctgggtcgt
     4501 ggccaccgac cacgaactgg ccgaagttgt cgccggcggc gcggcggtcg ccagcgccca
     4561 ggacgatcac acagtccaag tccacgtcgg cggcctcgag ccggggcgcg agtacttcta
     4621 cggcttcgca gtcgccgccg agcattcgca gaccgggcgc acccgcaccc tcgccgccga
     4681 gcccgagcgg gtccgcttcg ccacctgctc ctgcgccaa
//
LOCUS       DASRIG010000006         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1048712, whole genome shotgun sequence.
ACCESSION   DASRIG010000006 DASRIG010000000
VERSION     DASRIG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048712"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFB52_00090"
     CDS             <1..469
                     /locus_tag="VFB52_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04760.1"
                     /translation="PRNDDSDSDGVSDGDEGAGTIVSFDAETGRLVIDLFADDEIAGF
                     VTDETRIKCERSDDDSATTSRSDGEDEPGDDDGGHGDEPGDDHSDDVSDDSSGPSDNS
                     GPGSEDSGHGSHDGDCDDSECGTDALQVGAVVDEAELDVRNGKATFEEVELAH"
     gene            complement(488..3112)
                     /gene="polA"
                     /locus_tag="VFB52_00095"
     CDS             complement(488..3112)
                     /gene="polA"
                     /locus_tag="VFB52_00095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZO04761.1"
                     /translation="MAKDPPQLFLIDGNSLAYRAFFALPESIGTSDGRPTNAIYGLAS
                     MLVKIIDEHHPQGVVVAWDAGMSGREKTYDLYKAQRKSRPDLLREQWPHLMPLVEAFG
                     YTNVRVEGYEADDVIASMVKQAREEGIEVMVVTGDRDAYQLVGEGVRVMSTSRGITET
                     KIYDVAAVEERYGVPPELITDMMGLRGDTSDNIPGVPGIGEKTATQLLQQFGSLEGVL
                     ESVDQISGAKRKQNLTEHADDARMSQQLATLQYDIETGVDLQAAMVSEPDRGALRDFM
                     REFELRAVLERLEEALPEGDAVPGRKVETEIAIEAEEGGLEDIPAGPVALALADHTWA
                     AADAERTITGSASHDELAVALGAKPIVGHDLKSLGGGRHGLLAAAAREGVELSLDHDT
                     LLAAYLLEPQRRTYDLVELAADAGIGLAEGKSPTTEEDDGQLALGEELEAGLDPAEEA
                     RLVAALAEVQRPRIESFGLESLLREVELPLIEVLAAMEREGLKLDAERLAEVGAGFGE
                     RIDTLEKEIYELADEEFTIGSPQQVGRVLFEKLELTRKRRGKTGYSTDARVLAQIRDE
                     HPIVEKIESWRELTKLKNTYLDSLPDLIDPGDGRIHTTFNQAATTTGRLSSTNPNLQN
                     IPIRTEIGRPVRACFVAEQGAQLLSADYSQVELRVLAHVADEGVLKDVFHKGEDVHAA
                     TAAEVFEIGRDEVDVGQRSKAKMVNFGIVYGLTGFGLADRLNIPRKEGEEFVARYLER
                     FPAVRAFREQIVEQAQEEGFVTTLMGRRRSIPELRSGNPNTRRLGERLAVNTVIQGTA
                     ADIIKVAMVRCQRALREAGGETRLVLQIHDELLFEGPPGEMEAAAELVEREMCAAYQL
                     DPPLEVDIGVGADWLDAK"
BASE COUNT          455 a         1178 c         1083 g          523 t
ORIGIN      
        1 cccccgcaac gacgactccg acagcgacgg cgtctccgac ggcgacgagg gcgccggcac
       61 gatcgtctcc ttcgacgccg agaccggccg cctcgtgatc gacctcttcg ccgatgacga
      121 gatcgccggc ttcgtcaccg acgagacccg gatcaagtgc gagcgctccg atgacgacag
      181 cgccaccacc tcccgctccg acggcgagga cgagcccggc gacgacgacg gtggccacgg
      241 cgacgagccg ggcgacgatc acagcgacga cgtctccgac gacagcagcg gcccgagcga
      301 caactccggc cccggctcgg aggactccgg tcacggcagc cacgacggcg actgcgatga
      361 ctccgaatgc ggcaccgacg ccctccaggt cggcgcggtg gtcgacgagg ccgagctcga
      421 cgtccgcaac ggcaaggcga ccttcgagga ggtcgagctc gcccactgag gcgggtccgg
      481 aggccgacta cttggcgtcg agccagtcgg ctccgacgcc gatgtccacc tccagcggcg
      541 ggtcgagctg gtacgcggcg cacatctcgc gctcgaccag ctcggccgcc gcctccatct
      601 cccccggcgg gccctcgaac agcagctcgt cgtggatctg gaggaccagc cgggtctccc
      661 ccccggcctc gcgcagcgcc cgctggcagc gcaccatcgc caccttgatg atgtccgcgg
      721 cggttccctg gatcacggtg ttgaccgcga ggcgctcacc aagccggcgc gtgttcgggt
      781 tgcccgagcg cagctccggg atcgagcgcc gccgccccat cagcgtggtc acgaagccct
      841 cctcctgtgc ctgctcgacg atctgctcac ggaaggcgcg cacggcgggg aagcgctcca
      901 ggtagcgggc gacaaattcc tcgccctcct tgcgcgggat gttgaggcgg tcggccaggc
      961 cgaaaccggt cagcccgtag acgatcccga agttgaccat cttcgccttc gagcgctggc
     1021 cgacgtcgac ctcgtcgcgg ccgatctcga agacctcggc ggcggtcgcg gcgtgcacgt
     1081 cttcgccttt gtggaagacg tctttgagca cgccctcgtc ggcgacgtgg gcgagcaccc
     1141 gcagctcgac ctggctgtag tcggcggaga gcagctgggc gccctgctcg gcgacgaagc
     1201 aggcccgcac cgggcggccg atctcggtcc tgatcgggat gttctgcagg ttcgggttgg
     1261 tgctggagag gcggccggtg gtcgtcgccg cctggttgaa ggtggtgtgg atgcgcccgt
     1321 cgccggggtc gatcaggtcc ggcaaggaat cgagataggt gttcttcagc ttggtcagct
     1381 cgcgccagga ctcgatcttc tcgacgatcg ggtgctcgtc gcggatctgg gcaagcaccc
     1441 gggcgtcggt cgagtagccg gttttgccgc gccgtttccg cgtcagctcg agcttctcga
     1501 agaggacgcg gccgacctgc tggggcgagc cgatcgtgaa ctcctcgtcg gccagctcgt
     1561 agatctcctt ttcgagcgtg tcgatccgct cgccgaagcc ggccccgacc tcggccaggc
     1621 gctcggcgtc gagcttcagc ccctcgcgtt ccatcgccgc caggacctcg atcagcggca
     1681 gctcgacctc gcgcagcaag gattcgagcc cgaacgactc gatccgcggt cgctgcacct
     1741 cggccagcgc cgcgaccagg cgcgcttcct cggccgggtc gagcccggcc tcgagctcct
     1801 cgccgagcgc cagctgcccg tcgtcctcct cggtcgtcgg cgatttgccc tcggcgaggc
     1861 cgatcccggc gtcggcggcg agctcgacca ggtcgtaggt gcggcgctgg ggctcgagca
     1921 gataggcggc cagcagcgtg tcgtgatcga ggctcagctc gaccccctcg cgggcggcgg
     1981 cggcgagcag accgtggcgc ccgcccccga gcgacttcag gtcgtggccg acgatcggct
     2041 tggcgccgag cgcgacggcg agctcgtcgt ggctcgccga gccggtgatc gtgcgctccg
     2101 cgtcggccgc ggcccaggtg tggtcggcga gcgccagcgc caccgggccg gcggggatgt
     2161 cctccagccc accctcctcg gcctcgatcg cgatctcggt ctcgaccttg cgcccgggca
     2221 ccgcgtcgcc ctcgggcagc gcttcttcca ggcgctccag caccgcgcgc agctcgaact
     2281 cgcgcatgaa gtcgcgcagc gcgccgcggt cgggttcgga gaccatcgcc gcctgcaggt
     2341 cgaccccggt ctcgatgtcg tactggagcg tcgccagctg ctgcgacatg cgcgcgtcgt
     2401 cggcgtgctc ggtgaggttc tgtttgcgtt tggcgccgga gatctggtcg acgctttcca
     2461 gcaccccctc cagcgagccg aactgctgca gcagctgcgt cgccgttttc tcgccgatcc
     2521 cggggacgcc ggggatgttg tccgaggtgt cgccgcgcag gcccatcatg tcggtgatca
     2581 gctccggcgg caccccgtag cgctcttcga ccgcggcgac gtcgtagatc ttcgtctcgg
     2641 tgatcccgcg cgaggtgctc atcacccgca ccccctcgcc gaccagctgg taggcgtccc
     2701 ggtcgccggt cacgaccatc acctcgatcc cctcttcgcg ggcctgcttg accatcgagg
     2761 cgatcacgtc gtcggcctca tacccctcga cccgcacgtt ggtgtagccg aaagcctcga
     2821 ccagcggcat caggtgcggc cactgctcgc gcaacaggtc cggtcgcgat ttccgctgcg
     2881 ccttgtagag gtcgtacgtt ttctcccgcc ccgacatccc cgcatcccaa gcgaccacaa
     2941 ccccctgcgg atggtgctcg tcgatgattt tcaccagcat cgaagcgagc ccgtagatcg
     3001 cattcgtcgg gcggccgtcc gaggtcccga tcgactccgg cagcgcgaag aaagcgcggt
     3061 aagcgagcga gttgccgtcg atcaggaaga gctgcggtgg gtccttggcc atcggccgac
     3121 cactctatga ggctcgaact acggaccgcc gcccaggact tcgcgcaaat ccgcaactac
     3181 cgattcgggg tggttgcgga tctgcgcgaa gtcctgggcg ccgctccgta gtccccgcc
//
LOCUS       DASRIG010000007         7994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_5517, whole genome shotgun sequence.
ACCESSION   DASRIG010000007 DASRIG010000000
VERSION     DASRIG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5517"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFB52_00100"
     CDS             <1..447
                     /locus_tag="VFB52_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZO04762.1"
                     /translation="ATTDTAAGKPESKRKIRVVVAKPGLDGHDRGAKIIARALRDAGM
                     EVIYTGLHQTPEQIAETVIQEDADAVGLSILSGAHMTLVPKVVELLAAQGVDDVLVTV
                     GGTIPADDIPQLKELGVAEVFTPGAGTDEIVDFIRGAAAERHAQGA"
     gene            631..1422
                     /locus_tag="VFB52_00105"
     CDS             631..1422
                     /locus_tag="VFB52_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZO04763.1"
                     /translation="MKICVLVKEVPDAAVQKRIDPGTMRLDRSGEKNLNPFDTHAIEA
                     AMQIREGGAIPVEEIVAVTMGPDSASRALEKAVSLGADRMLHLSDEALAGSDVCATGY
                     ALAKLLESESPDLVLLGQQSDDGECYTIGAVVADHLQMPSLTQVIKIDVNGDKLRCER
                     QAEYGYDTVEVELPAVISVGDAINEPRYPSLKAIMGAKKKPKDKKAVGDVGIEAGKVG
                     AEGSQTTVIALNPPPAKEAGEIIEDEDTNETVEKIVAWLDERKLI"
     gene            1423..2397
                     /locus_tag="VFB52_00110"
     CDS             1423..2397
                     /locus_tag="VFB52_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZO04764.1"
                     /translation="MAGILVYALHDDEGNFNKNSLGAISEAAKLAGEIGGEASALVVG
                     DIADDACASLGNWGAAKVYRAKSVPEGLAQPIIDAMAKVIQENGISYALFGGGLLGFE
                     IGAGLTARLGAGVTMEVTAVKVQDGKLVVERPILQDSQIADVGYVSEPGIIIGRLNAF
                     DAKESGGTAEVVDVDVELSAWSSKATLVQRGEQRGADVNIEDADILVGGGRGLGAKEG
                     FEQCEELAAAMGGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAAGVSGAIQHKV
                     GMQNSENILAINKDPNAPIFEFSDLGVVGDLHKIVPKLTEAIKAKKGG"
     gene            2402..4105
                     /locus_tag="VFB52_00115"
     CDS             2402..4105
                     /locus_tag="VFB52_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HZO04765.1"
                     /translation="MPAVAPSEFPPPFDSADVIGPPTDAPDERIEVGVAIVGGGPAGL
                     ACANKVLQLLENEPELTEKLGEVPVAVIEKGKVAGAHLLSGANMKPSAMEELFPDLPK
                     EEWPVYQEVTKDAVYLLTSKAAFPLKPMPPNFKNHGNYVTSVAKLGRFLGEKAEEAGA
                     YVLSETAATKLLVEDRVVKGVRSGDRGRGKDGEELGNFEPGSDVIAKATVLAEGTVGH
                     LTYAAYDYFDMHGADPMRWELGVKEVWEVKEPLDRVIHTMGWPLRKSPRFNEFGGSFI
                     YPMGEDKICIGLVAGLDYTDATFSVHDALQLLKTHPFIKKLLDGGKRVAWGAKTIPSG
                     GYFAMPKRLAVPGMVVAGDAASMVNVPTLKGVHYAMHAGIYAAETIVDALKKDIESVN
                     FEAYDEKVHNSLIEKDLYESRNMRQPFSKGFFLGGAFASAMTVTKGNFPGGHWKQEHD
                     SEVEMNLGAAGDKYPKADNELTFNKLDSVFLSGNATRDDAPNHVRIQSRVPREIAQTW
                     VSMCPAQVYEIPDDQLENGAAEVDVHVTASNCVQCGAITAKGGRLTLPEGGDGPLYQE
                     T"
     gene            4173..4382
                     /locus_tag="VFB52_00120"
     CDS             4173..4382
                     /locus_tag="VFB52_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04766.1"
                     /translation="MFGTDSILDQFAALSAFLATGVALGGFLGHAMPALRGESEKRLR
                     QATAEGGLYALVGVVVLSAILFLTG"
     gene            4379..4582
                     /locus_tag="VFB52_00125"
     CDS             4379..4582
                     /locus_tag="VFB52_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04767.1"
                     /translation="MTEKAAKRILLGLTVLAAAALAVGVRVFQVGDEALVYGTMSIVI
                     VSSLAVGQITVHYGPSRQHRDDD"
     gene            4613..5476
                     /locus_tag="VFB52_00130"
     CDS             4613..5476
                     /locus_tag="VFB52_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04768.1"
                     /translation="MLVVGIVCAVGASCLYNTSIALQALQVRDVHHEHSLKPSLLGKL
                     LQNKRWLGATALGLAGWPLEIVALLFAPLTVVQPCLASGLIVLLWLGVRKLGETPGKR
                     EYLSVAAITAGVIGVAWAAPDRTTEHAATWAIALSLGIVAIPIVLPYLLRGRAVAAGM
                     IAVISAGCGYAWTAIASKLLTDELSEGALLIAVAWLATAVLSEALALLSEMSALQKRP
                     ATHVAPVMFAFQVLVPVILAPLIFMEKWSTTPLDGGALVFFMLVALAGVILLAGSKAV
                     GAVIDTAHSND"
     gene            5603..6640
                     /locus_tag="VFB52_00135"
     CDS             5603..6640
                     /locus_tag="VFB52_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZO04769.1"
                     /translation="MGPAETASPDATKFAIGFDHRDRARLFELWTEVLDSEQWSEGAK
                     LKAFETCWAEWNGLPSLGLSSWAGGALAALEFAGVKGETVLCPSNTFMASPLAAIGAG
                     AEVEFVDCNREDLCMSFEDFERKAEQHKPRAAILVHIGGHIAFDAEKIAEYCAAEGIF
                     LLEDCAHAHGADWNGNRPGAYGDAGVYSLYATKTVSTGEGGVLVSGNPELIEFAAKFR
                     NYGKFEHEVDGLNFRMSEFTAALALVQAERMEEIVAWKNEYAREHLDPVHPARLELPE
                     GMTSGFYKYIVFEELEKSTGKVYADPCHKIMGRSDKLPNTDWVAENHWCVPLYYRPES
                     REAALGKEAVA"
     gene            6637..>7994
                     /locus_tag="VFB52_00140"
     CDS             6637..>7994
                     /locus_tag="VFB52_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO04770.1"
                     /translation="MRVLVTGGGGFIGSHVVDRLLEKGMTPRIFDLSASPYHSPLEVE
                     TFTGSITDAANLDLAMRDCDAVIHLAAVADVGHVLADPVLAEEVNTRGTLNVLEAACR
                     AKVGRVVYGSTTWVYSDCPEQEVDEETAIPAPRHLYTATKLAGETYCSGYAELYDLES
                     TVLRFGIPYGPRARAAGVVAKFTDLSFEGKALTIAGDGSTTRSFIYVEDLADGIVAAL
                     APAAAGRTYNLSGDEVVTILEIAERVQENVDTCEIEHTPPRPGDFPGKSISNERALAE
                     LGWKAETSFKDGVRKYVEWVRGTTRPPDPVPGKTPSMNGNEHAAGALLAGVSQFEEKD
                     PKVLVLTADIGEGHDLPARIIKADLEAEVPNATVEIDNGLEAMGKLLSAVVRGGSRFT
                     FRWANWLFDVQYWLITKFAPTRWLAHHLMYLFGARGLVRLIAKHDPDVVISTYPGTTA
                     VLG"
BASE COUNT         1403 a         2757 c         2625 g         1209 t
ORIGIN      
        1 gcaaccaccg acacggctgc cggcaagccg gaatccaagc ggaagatccg cgtcgtcgtg
       61 gccaaacccg gcctcgacgg ccatgaccgc ggcgcaaaga tcatcgcgcg ggccctgcgt
      121 gacgcaggga tggaggtcat ctacacgggc ctgcaccaga cccccgagca gatcgccgag
      181 accgtgattc aggaggacgc cgacgcggtc ggcctctcga tcctctccgg cgcccacatg
      241 accctggtcc cgaaagtggt cgagctgctc gccgcccagg gggtcgacga cgtgctcgtc
      301 accgtcggcg gcacgatccc ggccgacgac atcccgcagc tgaaggagct cggcgtcgcc
      361 gaggtcttca cgccgggcgc cggcaccgac gagatcgtcg atttcatccg cggcgccgcc
      421 gcggagcgcc acgcccaggg cgcctagaac aagttgcgca tcaacttctc tacacggatt
      481 cgtgcatagt tccgccgttc cgggtaatat cttgattgac tcgtcagtca accggtccgg
      541 caaccgataa catgcccggc cgctacgggc ccgcgaggcg caaaccgcag cccgcaagac
      601 cctgagaatc gtttcccaag gaggcttcac ttgaagatct gcgtcctcgt caaggaggtt
      661 ccagacgccg cggtgcagaa gcgcatcgac cccggcacca tgcgcctgga ccgttccggc
      721 gagaaaaacc tcaacccgtt cgacacgcac gcgatcgagg ccgcgatgca gatccgtgag
      781 ggcggtgcga tcccggttga ggagatcgtt gccgtgacca tgggtccgga ttccgcctcc
      841 cgcgcgctcg agaaggctgt ctcgctcggc gccgaccgca tgctccacct ctccgatgag
      901 gcgcttgccg gctccgacgt ctgtgccacc ggttacgccc tggcgaagct gctcgagtcc
      961 gagtcgccgg acctggtcct gctcggtcag cagtccgatg acggcgagtg ctacacgatc
     1021 ggcgcagtgg tcgccgacca cctgcagatg ccctcgctga cccaggtgat caagatcgac
     1081 gtcaacggcg acaagctccg ctgcgagcgc caggccgagt acggctacga caccgttgag
     1141 gtcgagcttc cggccgtgat cagcgtcggt gacgcgatca acgagccgcg ctacccgagc
     1201 ctcaaagcga tcatgggtgc gaagaaaaaa ccgaaagaca agaaggctgt cggcgacgtc
     1261 ggcatcgagg ccggcaaggt cggcgccgag ggctcgcaga ccaccgtgat cgccctcaac
     1321 cccccgcccg ccaaggaagc cggggagatc atcgaggacg aggacaccaa cgagacggtc
     1381 gaaaagatcg tcgcttggct ggacgagagg aagctgatct agatggcagg cattctggtt
     1441 tacgccctcc acgacgacga gggcaacttc aacaagaact cgctcggggc gatctccgag
     1501 gcggcgaagc tggccggtga aatcggcggc gaggcttcgg ccctggtcgt cggtgacatc
     1561 gccgacgacg cttgcgcttc gctcggcaac tggggcgccg ccaaggtcta ccgggccaag
     1621 agcgtgccgg agggcctcgc ccagccgatc atcgacgcga tggcgaaggt gatccaggag
     1681 aacggcatct cctacgcgct gttcggcggc ggcctgctcg gcttcgagat cggggccggc
     1741 ctcaccgccc gcctcggcgc cggtgtgacc atggaggtca ccgcggtcaa agtccaggac
     1801 ggcaagctgg tcgtcgagcg gccgatcctc caggactcgc agatcgccga cgtcggttac
     1861 gtcagcgagc ccgggatcat catcggccgc ctcaacgcct tcgacgcgaa ggagagcggt
     1921 ggcacggctg aggtcgtcga cgtcgacgtc gagctctccg cctggtcgtc caaggcgacc
     1981 ttggtgcagc gcggtgagca gcgcggcgcc gacgtcaaca tcgaggacgc cgacatcctc
     2041 gtcggcggcg gccgcgggct cggcgccaaa gagggcttcg agcagtgtga ggaactcgcc
     2101 gcggcgatgg gcggcgccgt tgccgcgacc cgcgcggtcg tcgacgccgg ttggtacccc
     2161 tacgcggccc agatcggcca gaccggcaag accgtggcgc cgaagctgta cctcgctgcc
     2221 ggcgtctccg gggcgatcca gcacaaggtc gggatgcaga actcggagaa catccttgcg
     2281 atcaacaagg atccgaacgc gccgatcttc gagttctcgg acctcggcgt ggtcggcgat
     2341 ctgcacaaga tcgtccccaa gctgaccgag gcgatcaaag ccaagaaggg cggctgagca
     2401 gatgcccgcc gtcgcaccca gcgagttccc gccgccgttc gactcggccg acgtgatcgg
     2461 tccgccgacc gacgcgccgg acgagcgaat cgaggtagga gtcgcaatcg tcggcggcgg
     2521 gcccgcaggc ctcgcctgcg ccaacaaagt tctgcagctg ctcgagaacg agccggagct
     2581 gaccgagaag ctcggtgaag tcccggtcgc ggtgatcgag aagggcaaag tcgccggggc
     2641 gcacctgctc tccggggcga atatgaagcc ctcggcgatg gaagagctgt tcccggacct
     2701 gccgaaggag gagtggccgg tctaccagga ggtgaccaag gacgcggtct acctgctgac
     2761 ctcgaaggcg gccttcccgc tgaaaccgat gccgccgaac ttcaaaaacc acggcaacta
     2821 cgtcacctcg gtcgcgaaac tgggccgctt ccttggcgag aaagccgaag aagccggcgc
     2881 ctacgtgctc agcgagaccg cggcgaccaa gctgctggtc gaggaccgcg tggtcaaggg
     2941 cgtgcgctcc ggcgaccgcg gccgtggcaa ggacggcgaa gagctcggca acttcgagcc
     3001 cggctccgac gtgatcgcga aagcgaccgt gctcgccgag ggcaccgtcg gccacctcac
     3061 ctacgccgcc tacgactact tcgacatgca cggcgccgac ccgatgcgct gggagctcgg
     3121 ggtcaaagag gtctgggagg tcaaagagcc gctcgaccgc gtgatccaca cgatgggctg
     3181 gccgctgcgc aaatcgccgc gcttcaacga gttcggcggc agcttcatct acccgatggg
     3241 cgaggacaag atctgcatcg gtctggtcgc cggcctcgac tacaccgacg cgaccttctc
     3301 cgtccacgac gcgctgcagt tgctgaagac gcaccccttc atcaagaagc tgctcgacgg
     3361 aggcaagcgg gtcgcctggg gcgcgaagac gatcccctcg ggcggttact tcgcgatgcc
     3421 caagcgcctc gccgtgcccg gcatggtcgt cgccggcgat gccgccagca tggtcaacgt
     3481 gccgaccctg aagggcgtcc actacgcgat gcacgccggg atctacgccg ccgaaacgat
     3541 cgtcgatgcc ctcaagaagg acatcgagag cgtcaacttc gaggcctacg acgagaaggt
     3601 ccacaactcg ctgatcgaga aggacctcta cgaatcgcgc aacatgcgcc agccgttctc
     3661 gaaagggttc ttccttggcg gcgccttcgc ctcggcgatg acggtgacca aaggcaactt
     3721 cccgggcggg cactggaagc aggagcacga ctccgaggtc gagatgaacc tcggcgcggc
     3781 cggcgacaag tacccgaagg ccgacaacga gctgaccttc aacaagctcg actcggtctt
     3841 cctctccggc aacgcgaccc gcgacgacgc ccccaaccac gtccggatcc agagccgcgt
     3901 gccccgcgag atcgcccaga cctgggtctc gatgtgcccg gcccaggtct acgagattcc
     3961 cgacgatcag ctcgagaacg gggcggccga ggtcgacgtc cacgtcaccg cctccaactg
     4021 cgtccaatgc ggcgcgatca ccgccaaggg cggccgcctc accctccccg agggcggcga
     4081 cggcccgctt taccaggaga cgtaggcaaa gtcgcacaga atcggcgcgt tctgcgcgcc
     4141 acgcgtgaac cggcccggcc tagggtcggt tcatgttcgg caccgactcg atcctcgacc
     4201 agttcgcggc tctgtccgcg ttcctggcga ccggtgtcgc gctgggggga tttctcgggc
     4261 acgcgatgcc cgcgctgcgc ggcgagagtg agaaacgttt gcgtcaggcg acggccgaag
     4321 ggggtctcta cgccctcgtg ggtgtagttg ttttgtctgc aatacttttc ctcaccggat
     4381 gactgaaaaa gccgccaagc gaatccttct gggcctcacc gtgcttgcgg ctgcggccct
     4441 ggccgttggt gtgagggtct tccaggttgg agacgaagcg ctcgtgtacg gaacgatgtc
     4501 gatcgtgatc gtcagttccc tcgccgtggg gcagataacc gtgcactacg ggccctcacg
     4561 tcaacaccgc gacgacgact agccgaccgc cggcgccgga tatggtcgcg gcgtgctcgt
     4621 cgtcgggatc gtctgcgcgg taggggcctc gtgcctctac aacacctcga tcgcgctgca
     4681 ggcgctgcag gtgcgcgacg tgcaccacga gcactcgctg aagccgtcct tgctcggcaa
     4741 gctgctgcag aacaagcgat ggctgggggc gacggcgttg gggctggcgg gctggccact
     4801 ggagatcgtg gcgctgctgt ttgcgccgct gaccgtggtc caaccctgcc tcgccagcgg
     4861 cctgatcgtg ttgctgtggc tcggggtgcg gaagctgggg gagaccccgg gcaagcgtga
     4921 gtacctgtcg gtggcggcga tcaccgccgg cgtcatcggc gttgcctggg ccgcccccga
     4981 tcgcacgacc gagcacgccg ccacctgggc gatcgcgctc tcgctgggga tcgtcgcgat
     5041 cccgatcgtg ctgccgtacc tgctgcgggg ccgggcggtc gcggcgggga tgatcgcggt
     5101 gatcagcgcc ggctgcggct acgcctggac tgcgatcgcg agcaagctgc tgaccgacga
     5161 actctccgag ggcgcgttgc tgatcgcggt cgcctggctg gccacggcgg tcctctcgga
     5221 ggcgttggcg ctgctcagcg agatgagcgc cctgcagaaa cgcccggcca cgcacgtggc
     5281 gccggtgatg ttcgcctttc aggtcctggt gccggtgatc ctggcgccgc tgatcttcat
     5341 ggagaagtgg tcgacgacgc ccctcgacgg cggggcgctc gtcttcttca tgctcgtcgc
     5401 gctcgccggg gtgatcctgc tggccggttc gaaggccgtc ggcgccgtaa tcgacaccgc
     5461 acactcgaac gattgaacgt ccagagggcg cgctgcgacg ccgctcgcgc tggtagcctc
     5521 cccgccgctc ggccatcgca taaccagtca actttccccg cccgggctca gatctaaacc
     5581 accccttcag caaggagcgc cagtgggtcc cgcagagacc gccagccccg acgcgaccaa
     5641 gttcgcgatc ggcttcgatc accgcgaccg cgccaggttg ttcgagctct ggaccgaggt
     5701 cctcgactcc gagcagtggt cggagggcgc caagctgaag gccttcgaga cctgttgggc
     5761 ggagtggaac gggctgccca gcctcggcct ctccagctgg gccggcggcg ctctcgccgc
     5821 gctcgagttt gccggggtca aaggggagac ggtgctctgc ccgtccaaca ccttcatggc
     5881 atcgccgctg gcggcgatcg gggcgggcgc cgaggtcgag ttcgtcgact gcaaccgcga
     5941 ggacctctgc atgtccttcg aggacttcga gcgcaaggcc gagcagcaca agccgcgcgc
     6001 cgcgatcctc gtccacatcg gcggccacat cgccttcgac gccgagaaga tcgccgaata
     6061 ctgtgccgcc gaagggatct tcctgctcga ggactgcgcc cacgcccacg gcgccgactg
     6121 gaacggcaac cgccccggcg cctacggcga cgcaggcgtc tattcgctct acgcgaccaa
     6181 gaccgtctcc accggcgagg gcggggtgct ggtctccggc aacccggagc tgatcgagtt
     6241 cgccgccaag ttccgcaact acggcaagtt cgagcacgag gtcgatggac tcaacttccg
     6301 gatgagcgag ttcaccgccg cgctcgccct ggtccaggcc gaacggatgg aggagatcgt
     6361 cgcctggaag aacgagtacg cgcgcgagca cctcgacccc gttcacccgg cccggctgga
     6421 gctgcccgag ggcatgacct ccggcttcta caagtacatc gtcttcgaag agctcgagaa
     6481 gagcaccggc aaggtctacg ccgacccctg ccacaagatc atgggccgct ctgacaagct
     6541 gccgaacacc gactgggtgg ccgagaacca ctggtgcgtg ccgctctact accgccccga
     6601 gagccgtgag gccgcgctgg gcaaggaggc cgttgcatga gggtcctggt tacgggcggg
     6661 ggcggcttca tcggctccca tgtagtcgat cgcctgctgg agaaggggat gacgccgcgg
     6721 atcttcgatc tcagcgcctc gccctaccac tcgccgctcg aggtcgagac cttcaccggc
     6781 tcgatcaccg acgccgccaa cctcgacctc gcgatgcgcg attgcgacgc ggtcatccac
     6841 ctcgccgcgg tcgccgacgt cggccacgtg ctcgccgacc cggtgctggc cgaggaggtc
     6901 aacacgcgcg gcaccctgaa cgtgctcgag gccgcctgcc gggcgaaagt cggccgggtc
     6961 gtctacggct cgaccacctg ggtctacagc gactgccccg agcaggaggt cgacgaggaa
     7021 accgcgatcc cggcgccacg ccacctctac accgcgacca agctcgccgg cgagacctac
     7081 tgctccggct acgccgagct ctacgacctc gagagcacgg tcctgcgctt cggcatcccc
     7141 tacggcccgc gcgcccgcgc cgccggcgtc gtcgccaagt tcaccgacct ctccttcgaa
     7201 ggcaaagcgc tgacgatcgc cggcgacggc agcacgaccc gcagcttcat ctacgtcgag
     7261 gacctcgccg acgggatcgt cgccgcgctc gcgcccgcgg ccgccggccg cacttacaac
     7321 ctctccggcg acgaggtcgt gacgatcctc gaaatcgccg agcgggtcca ggaaaacgtc
     7381 gacacctgcg agatcgaaca cacgccgccg cgcccgggcg actttcccgg caagtcgatc
     7441 tccaacgagc gcgcgctcgc ggagctcggc tggaaagcgg aaaccagctt caaagacggc
     7501 gtccgcaagt acgtcgaatg ggtgcgcggg acgacccgcc cgccggaccc ggtgccgggc
     7561 aagacgccct ccatgaacgg caacgaacac gctgccgggg cgctgctcgc gggcgtctcc
     7621 cagtttgagg agaaggaccc gaaggtactg gtgctgaccg ccgacatcgg cgaggggcat
     7681 gatctgccgg cgcggatcat caaggccgac ctcgaagcgg aagtgccgaa cgccacggtc
     7741 gagatcgaca acggcctcga ggcgatgggc aagctgctct cggcggtcgt ccgcggcggc
     7801 tcccgcttca ccttccgctg ggccaactgg ctgttcgacg tccagtactg gctgatcacc
     7861 aagttcgccc cgacccgctg gctggcgcac cacctgatgt acctgttcgg cgctcgcggg
     7921 ctcgtccgcc tgatcgccaa gcacgacccg gacgtggtca tctcgaccta ccccgggacc
     7981 accgcggtgc tcgg
//
LOCUS       DASRIG010000008         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2871300, whole genome shotgun sequence.
ACCESSION   DASRIG010000008 DASRIG010000000
VERSION     DASRIG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871300"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFB52_00145"
     CDS             <1..564
                     /locus_tag="VFB52_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04771.1"
                     /translation="ANVVPGGETLNPSTYELLAGIHSISAPEVLVLPSSSNVVMAAER
                     ACELSEKPARVVPALSQQASLLALVELDPSGSADENATRLEGALAGVATGGVAPAARD
                     DAQGRFKKGDAVGFADGEIVAWGGTGSTLTSTVQHLASDAEIVTVIAGEGAPIPLSEI
                     DTHVPEGVEVETHEGGQPSWWWLLAAQ"
     gene            564..>2657
                     /gene="recG"
                     /locus_tag="VFB52_00150"
     CDS             564..>2657
                     /gene="recG"
                     /locus_tag="VFB52_00150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZO04772.1"
                     /translation="MRSFAGERELTPAELRAAPIHWPRPSVLDAGLQALQGVGPKSAE
                     TASAAGIATVWDLLLRLPRRHRDMQLRPLAEIGVGETGTTLVEVLGSQPRPFRKGRLT
                     FVGVKVGDGSGHVRATWFNQPWVASKLVPGARLLVTGKRTERGLAVNQWELVSSGEEE
                     GETPEGGSFVPIHPATGDLPPQKIREWAERAVAFAPNVVEGLPAGLRTRRGLAGAPDA
                     FTAIHFPEDEEDLEAARRRLAFEELFLHQALLASRKRSQRTARPAPRFGKPGELVGRW
                     LDSLPFEPTGDQLQALDEIDADLDRGEPMQRLLMGEVGSGKTVVAVYAMLRALEAGYQ
                     AVLMAPTEVLAEQHALTLNRLLGAEAIPFSLLTGSTPAATRKRALGQLASGELSLLVG
                     THALIEPTVQFARLGLVVVDEQHRFGVEQRRALDAKGVEGMAPHVLHMTATPIPRTLS
                     LTAYGDLDTTALHELPAGRQPIETRLVEEEQRGEAFEFLRRRLREGRQAFVVCPLVAQ
                     SEKVQGKAAEVEAERLRGGELREFRVGVLHGQMRSEEKAAAMAAFAAGETDVLVATTV
                     IEVGIDVGNATVMVIEGAERFGVSQLHQLRGRVGRGEHPSQCLLFAEEAGELARRRLR
                     AVEETGDGFKLAEVDLALRGEGEILGTRQSGVPRFGVAELPDDLPLLLEAKREVIELL
                     ERYGSLEDAALGPLLE"
BASE COUNT          363 a          969 c          972 g          353 t
ORIGIN      
        1 gccaacgtcg tgcccggcgg cgagacgctc aacccctcga cctacgagct gctggcgggg
       61 atccacagca tctccgcccc ggaggtcctg gtcctgccct cgtcctcgaa cgtggtgatg
      121 gcggccgagc gcgcctgcga gctctccgag aagccggcgc gggtggttcc ggcgctctcc
      181 cagcaggcca gcctgctggc cctggtcgag ctcgacccct cgggttcggc cgacgagaac
      241 gcgacccggc tggagggggc gctggccggc gtcgccaccg gcggcgtcgc cccggccgcc
      301 cgcgacgacg cccagggccg cttcaagaaa ggcgacgcgg tcggcttcgc cgacggcgag
      361 atcgtcgcct ggggcggcac cggctcgacc ctcacctcga cggtccagca cctcgcctcc
      421 gacgccgaga tcgtcaccgt gatcgccggc gagggggcgc cgatcccgct ctcggagatc
      481 gacacccacg ttcccgaggg cgtcgaggtc gagacccacg aggggggaca gccgagctgg
      541 tggtggctgc tggcggcgca gtagtgcgtt cgttcgcggg cgagcgggag ctgacgcccg
      601 ccgagctccg cgccgccccg atccactggc cccgcccgag cgtgctcgac gccgggctgc
      661 aggcgcttca aggggtcgga ccgaaatcgg ctgagaccgc ctccgccgcc gggatcgcga
      721 ccgtctggga tctgctcctg cgccttcccc gccgccatcg cgacatgcag ctgcggccgc
      781 tggcggaaat cggcgtgggg gagacgggga cgacgctggt cgaggtgctg ggctcccagc
      841 cgcgcccctt ccgcaaaggt cggctgacct tcgtcggggt gaaagtcggc gacgggtccg
      901 ggcacgtgcg ggcgacctgg ttcaaccaac cgtgggtcgc ttcgaagctg gtcccgggcg
      961 cgcggctgct ggtgaccgga aagcggaccg agcgcgggct cgccgtcaac cagtgggagc
     1021 tggtctcctc cggtgaggag gagggcgaga cgcccgaagg cggctccttc gtgccgatcc
     1081 acccggcgac cggggacctg ccgccgcaga agatccgcga atgggccgaa cgggcggtcg
     1141 ccttcgcgcc gaacgtggtc gagggcctgc cggccggact gcgcacccgg cgcgggctcg
     1201 ccggcgcccc cgatgccttt accgcgatcc acttccccga ggacgaggag gacctggaag
     1261 cggcccgccg ccggcttgcc ttcgaagagc tgttcctcca ccaggcgctg ctggcgagcc
     1321 gcaaacgcag ccagcgcacc gcccgcccgg ccccgcgctt cggcaagccc ggcgagctgg
     1381 tcggacgctg gctcgactcg ctgccgttcg agccgaccgg cgaccagctg caggcgctcg
     1441 acgagatcga cgccgatctc gaccgcggcg agccgatgca gcgcctgctg atgggcgagg
     1501 tcggctccgg caagaccgtg gtcgccgtct acgcgatgtt gcgggccctg gaagccggct
     1561 accaggcggt gctgatggca ccgaccgagg tgctcgccga gcaacacgcg ctcaccctca
     1621 accgcctgct cggcgctgaa gcgatcccgt tctcgctgct caccggctcg accccggcgg
     1681 cgacccgcaa acgcgccctc ggccagctcg ccagcggtga gctctcgctg ctcgtcggga
     1741 cccacgcctt gatcgagccg accgtgcagt tcgcccgcct cggcctcgtc gtcgtcgacg
     1801 agcagcaccg cttcggggtc gagcagcggc gcgcgctcga cgccaagggg gtcgagggga
     1861 tggccccgca cgtcctccac atgaccgcga cgccgatccc gcgcacgctc tcgctgaccg
     1921 cctacggcga cctcgacacg accgcactgc atgagctgcc ggctgggcgg cagccgatcg
     1981 agacccgcct ggtcgaggag gaacagcgcg gcgaagcgtt cgagttcctg cgccgccgcc
     2041 tgcgcgaggg ccgccaggcg ttcgtcgtct gcccgctcgt cgcccagtcc gagaaagtcc
     2101 agggcaaagc ggccgaggtg gaggcagagc ggctgcgtgg cggggagctg cgcgagttcc
     2161 gggtcggcgt cctgcacggc cagatgcgct ccgaggagaa ggcggcggcg atggccgcct
     2221 tcgccgcggg cgagaccgac gtgctggtcg cgaccaccgt gatcgaggtc gggatcgacg
     2281 tcggcaacgc gaccgtgatg gtgatcgagg gagccgagcg cttcggcgtc tcccagctgc
     2341 accagctgcg cggccgggtc ggccgcggcg aacacccctc gcagtgcttg ttgttcgccg
     2401 aagaggcggg cgagctggcg cggcggcggc tgcgggcggt cgaggagacc ggcgacggct
     2461 tcaagctcgc cgaggtcgac ctggcgctgc gcggcgaggg cgagatcctc ggcacccgcc
     2521 agtccggggt gccgcgcttc ggcgtcgccg agcttcccga cgatctgcca ctgctgctcg
     2581 aggccaaacg ggaagtgatc gagctgttgg agcgctacgg ctcgctcgaa gacgccgccc
     2641 tcggcccgct gctcgag
//
LOCUS       DASRIG010000009         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1570433, whole genome shotgun sequence.
ACCESSION   DASRIG010000009 DASRIG010000000
VERSION     DASRIG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570433"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2871)
                     /locus_tag="VFB52_00155"
     CDS             complement(79..2871)
                     /locus_tag="VFB52_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO04773.1"
                     /translation="MADLLDREPEIQELDARVEAALAGEGSLAVIEGPAGIGKSRLLA
                     EARRRAGRSMRVLSARGGELEGEFPFGVVRQLFDPELTDPERREQLLAGAAAPAAAIF
                     GAPEPGDEGGQGASFASLHGAFWLVMELAEERPLLLCVDDLHWCDRPSLMFLAYLARR
                     IESQPVLLLTGLREAEPGTDPALLAELTQDPSATRVRPGPLSEAAIGALLGERLGAEP
                     APAFVAACREATGGNALLLSQLVTALRGEGVQPDAEHVARVTEIGPRAVSRTVLLRLA
                     RLPEEAKAVAQAVAVLGDGAALPAVIELAGVDEAAAAAATRELSRAEILRPEPPLGFV
                     HPLVRDAVYHELSPGERELEHARAAALLRDGGAPVDQVAAQLLHTSPRGEAWAAELMW
                     EAGRAAMHAGAAESAAAYLRRALAELPGDGERGRLLFELGAAEALTSGAPAQEHLTLA
                     YEELEDPTARAIAAGMLGRTLLFMGAPEEAAATARRAADELPPELEDVRLSLEALESI
                     TVFFGAEQRDRLTRLREFRRAPSGGPGAHGVVSIAAWEALCTDGTAEECTALALAALE
                     GGELRAADPALLPFGAIVSLVVADRPEVVDVWKEFLDDAHRRGSLLSTSSVLLWHGYS
                     HLRRGDLAAAEDSMRSADEMFTLWGHDEYARINSRSLMAEVMCEQGRTEEAERLLELV
                     GEVAPGTHATSNWLTARFALLAATGRAEEALAVADELAAQCAAVPDPSRLWWRSLKAE
                     ALDRLERTDEGIELAREQLEVARAFGAPRWTGRALRVLGTLERDDGIEALRESVAVLE
                     GSTARLEQAKALAALGSGLRRARQPSEAREPLRQALEQAGICGATALSEQVRTELHAT
                     GSRPRNDALSGVGSLTPSERRVADLAAAGNTNREIAQQLYVTPKTVEVHLSNTYRKLG
                     IRSRRELERALAGVAS"
BASE COUNT          355 a         1189 c         1099 g          363 t
ORIGIN      
        1 gaccgggacc cgaaggtttt ggatagagaa cccctaaggg gtgcggagcc tgggcgagtt
       61 actcacgcac gtgggtgact aactcgccac gcccgcgagc gcccgctcga gctcgcggcg
      121 tgagcggatt cccagcttcc gataggtgtt cgagaggtgg acctcgaccg tcttcggggt
      181 cacgtagagc tgctgggcga tctcgcggtt ggtattgccc gccgccgcca ggtcggcgac
      241 gcggcgctcg ctcggggtca gcgacccgac tccgctgagc gcgtcgttgc gcggcctgga
      301 cccggtcgcg tgcagctcgg ttcgcacctg ctcggagagc gcggtggcgc cgcatatccc
      361 ggcctgctcg agcgcctggc gcagcggctc gcgcgcctcg ctcggctgcc gcgcccggcg
      421 cagcccggag ccgagcgcgg ccagcgcctt cgcctgctcg agccgggcgg tcgagccctc
      481 cagcaccgcc acggactcgc gcagcgcctc gatcccatcg tcgcgttcga gcgtccccag
      541 gacccgcagc gcgcgtccgg tccagcgggg ggcgccgaag gcccgcgcca cctccagctg
      601 ctcgcgcgcc agctcgatcc cctcgtcggt ccgctccagc cggtcgagcg cctcggcctt
      661 cagcgagcgc caccagagcc ggctcggatc gggcacggcg gcgcactgcg cggccagttc
      721 gtcggccacg gccagcgcct cctcggcgcg gccggtggcg gcaagcaggg cgaagcgcgc
      781 ggtcagccag ttgctggtcg cgtgcgtgcc cggagcgacc tcgccgacca gttccaggag
      841 ccgctcggcc tcctcggtgc gcccctgctc acacatcact tcggccatca gcgagcggct
      901 gttgatccgc gcgtactcgt cgtggcccca gagggtgaac atctcgtcgg cgctccgcat
      961 cgagtcctcg gcggcggcga ggtcaccgcg gcgcaggtgg ctgtagccgt gccagagcag
     1021 gaccgaggag gtcgagagca gcgagccgcg gcggtgcgcg tcgtcgagga actccttcca
     1081 gacgtcgacc acctcgggcc ggtcggcgac gaccaggctg acgatcgccc cgaacggcag
     1141 cagggcgggg tcggcggcgc gcagctcccc gccctcgagt gcggccagcg ccagcgccgt
     1201 gcactcctcc gcggtgccgt cggtgcagag cgcctcccag gcggcgatcg agacgacccc
     1261 gtgcgctccc gggccgccgc tcggcgcccg gcggaactcg cgcaggcggg tcagccggtc
     1321 gcgctgctcg gcgccgaaga agacggtgat cgactccagc gcttccaggc tgaggcgcac
     1381 gtcctccagc tcgggcggca gctcgtcggc ggcccggcgt gcggtcgcgg ccgcctcctc
     1441 cggcgcgccc atgaagagca gcgtgcggcc cagcatcccc gcggcgatcg cccgcgcggt
     1501 cgggtcctcg agctcctcgt aggcgagggt caggtgctcc tgggcggggg cgccgctggt
     1561 cagtgcctcg gccgcgccga gctcgaacag cagccgcccg cgctcgccgt cgccgggcag
     1621 ctcggcgagc gcgcgccgca ggtaggcggc ggcgctttcc gcggcgccgg cgtgcatcgc
     1681 cgcgcgcccg gcctcccaca tcagctcggc cgcccaggcc tcgccgcgcg gggaggtgtg
     1741 gaggagctgg gcggcgacct ggtcgaccgg tgcgccgccg tcgcgcagca gcgccgccgc
     1801 gcgcgcgtgc tccagctcgc gctcgccggg ggagagctcg tggtagacgg cgtcgcggac
     1861 cagcgggtgg acgaagccga gcggcggctc cgggcgcagg atctcggccc gcgagagctc
     1921 gcgcgtggcc gccgccgcgg cggcctcgtc gacaccggcc agctcgatca ccgcgggtag
     1981 cgcggcgccg tcgccgagca ccgcgaccgc ctgcgccacc gccttcgctt cctcgggcag
     2041 ccgggccagc cgcagcagca cggtccgcga gaccgcccgg ggcccgatct cggtcacccg
     2101 cgcgacgtgc tcggcgtccg gctgcacgcc ctcgccgcgc agcgccgtga ccagctggct
     2161 gagcagcagc gcgttgccgc cggtcgcctc gcggcaggcg gcgacgaagg cgggggcggg
     2221 ctcggccccg agccgctcgc cgagcagcgc gccgatcgcc gcctcgctga gcggcccggg
     2281 ccggacccgg gtcgcgcttg ggtcctgggt cagctccgcg agcagcgccg ggtcggtccc
     2341 cggctcggcc tcgcgcaggc cggtcagcag caggaccggc tggctttcga tccgccgcgc
     2401 caggtaggcg aggaacatca gcgagggccg gtcgcaccag tgcaggtcgt cgacgcagag
     2461 cagcaacggc cgttcctcgg ccagctccat gaccagccag aaggcgccgt gcagggaggc
     2521 gaacgaggcg ccctggccgc cctcgtcgcc gggctcgggc gcgccgaaga tcgccgccgc
     2581 cggcgccgcc gccccggcca gcagctgctc gcgccgctcc gggtcggtca gctcggggtc
     2641 gaacagctgg cgcacgacgc cgaacgggaa ctcgccctcg agctcaccgc cgcgggccga
     2701 gagcacccgc atcgagcgcc cggcccgccg ccgcgcctcg gccagcagcc ggctcttgcc
     2761 gatccccgcc ggcccttcga tcaccgccag gctgccctct cccgcgagcg ccgcctcaac
     2821 ccgcgcgtcg agctcctgga tctccggttc ccggtcgagc agatccgcca cgccgcaatg
     2881 ctacgcgcgg ctggcgagcc agtcgacgat cccggcgacc gcctcccagt cctcgcgctc
     2941 acgcgcctcc accagctggc gcagggccag cggctcgacc gccatcagcg cctccgaccc
     3001 gctcgc
//
LOCUS       DASRIG010000010         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1829934, whole genome shotgun sequence.
ACCESSION   DASRIG010000010 DASRIG010000000
VERSION     DASRIG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829934"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFB52_00160"
     CDS             complement(<1..810)
                     /locus_tag="VFB52_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO04774.1"
                     /translation="MKLVERESELDALTALLDGLAEGRSRAALIEGPAGIGKTRLLAE
                     LRERARERRIPSLGARGSELEREFPFGVVRQMLEPALLARPDRSQLFEGAAEVAGAVF
                     APPGEDSLGGDASFAVLHGLYWLILDLVGDQPTLLTIDDLHWTDRPSLRALAYVVRRL
                     EGIPLLIVATMRSNEPGTDPALLAEIAQDPLTDRIQPGPLSPAAMPGLIERRLGTAPD
                     GAFAAACHEATGGNPLLLDELLKGLAAEGIEPSAERAGVVRDLGPRAVSRAV"
     gene            complement(807..1463)
                     /gene="rpiA"
                     /locus_tag="VFB52_00165"
     CDS             complement(807..1463)
                     /gene="rpiA"
                     /locus_tag="VFB52_00165"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HZO04775.1"
                     /translation="MPGTDDEKRLAAEAAAELVESGMTIGLGTGSTVAHLLPAIARRG
                     LGEIRCVATSVATSEQARELEIPVEEFNELARLDIAIDGTDEVTPDGWLIKGGGGAHL
                     REKIVAGAAARFVVIADSSKPVDVLRGPVPVEVFGFGAASTLARLGGEFEPRDVPRSP
                     DGGLIVDYMGAIGDPAELAARLEADPGVAAHGLFPPGMTSELFIARGGEVERTIIQAR
                     "
     gene            1575..3002
                     /gene="ahcY"
                     /locus_tag="VFB52_00170"
     CDS             1575..3002
                     /gene="ahcY"
                     /locus_tag="VFB52_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZO04776.1"
                     /translation="MTTSTATQDYKVADIGLAEFGRKEISLAEVEMPGLMQTREESAA
                     AQPLKGARITGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAAAGIPV
                     YAWKGETLEEYWWCTEQALLWPDGEGPNMILDDGGDATLLVHKGVEFEAAGAVPDPAG
                     AESEEFRIVLETLQRSLEADPQRWTKLADGIKGVTEETTTGVHRLYQLAETGDLLFPA
                     INVNDSVTKSKFDNLYGCRHSLVDGINRATDVMIGGKVAVVCGYGDVGKGCASSLRGQ
                     GARVIVTEIDPICALQAAMEGYEVRKLADVVETGDIFVTATGNKDIITAADMARMKHN
                     AIVGNIGHFDNEIDVAGLEAHPGVERINIKPQVDEWRFADGHSIVLLSEGRLLNLGNA
                     TGHPSFVMSNSFTNQVIAQIALFGESEQYAAKQVYVLPKHLDEKVARLHLAALGVDLT
                     ELTAEQSAYLGIPVEGPYKPEHYRY"
     gene            complement(3019..3276)
                     /locus_tag="VFB52_00175"
     CDS             complement(3019..3276)
                     /locus_tag="VFB52_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04777.1"
                     /translation="MPAATAAPSPIEGCTWKRHSKQVVKRVRRHGKQQRVKRLKHWWT
                     CQAQPSTFATLTLSPGSYRLYCSLYKHEAKGMEATLAVSAS"
     gene            complement(3366..3539)
                     /locus_tag="VFB52_00180"
     CDS             complement(3366..3539)
                     /locus_tag="VFB52_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04778.1"
                     /translation="MVGEFPGLAQLDQNDNVRVTSDFRAMYCSLLEEWLGHDAASVIP
                     GASSFERPVLVKA"
     gene            3649..4104
                     /locus_tag="VFB52_00185"
     CDS             3649..4104
                     /locus_tag="VFB52_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO04779.1"
                     /translation="MAGKTKDTTLFDAAAQLRLGIVRTARRLRQEAASEATGLTPTST
                     AALATIERHGPLTPSELAELERVKRPTVTRTLGCLEREGLIERTPDPADGRSTLISVN
                     PAGRERLRRLRGRKNAYLARRMREMPATDVEVLERAAEILERMLEDERR"
BASE COUNT          678 a         1517 c         1465 g          614 t
ORIGIN      
        1 caccgccctc gagaccgccc gcgggccgag gtcgcggacg acgccggcgc gctcggcgct
       61 tggctcgatc ccctccgccg ccaggccctt gagcagctcg tcgagcagca gcgggttgcc
      121 gccggtggcc tcgtggcagg cggcggcgaa ggcgccgtcg ggcgcggtcc cgagccggcg
      181 ctcgatcagc cccggcatcg ccgccgggct gagcggcccg ggctggatcc ggtcggtcag
      241 cgggtcctgg gcgatctcgg ccagcagcgc cgggtcggtg ccgggctcgt tgctgcgcat
      301 cgtggcgacg attagcagcg gaatgccctc cagccgccgc accacataag cgagcgcccg
      361 cagcgagggc cggtcggtcc agtgcaggtc gtcgatcgtc agcagggtgg gctggtcgcc
      421 gaccaggtcg aggatcagcc agtagagccc gtgcaggacc gcgaaggagg cgtcgccgcc
      481 gagcgagtcc tcgccgggcg gcgcgaagac cgcgccggcg acctcggcgg cgccctcgaa
      541 cagctggctg cggtcgggcc gcgccagcag cgccggctcg agcatctggc gcaccacccc
      601 gaacgggaac tcgcgctcca gctccgagcc gcgcgccccg agcgagggaa tgcgccgctc
      661 ccgcgcccgt tcacgcagct ccgccagcag ccgcgtcttg ccgatccccg ccggcccctc
      721 gatcagggcc gcccgcgaac gaccttccgc gagcccgtcg agcagcgcgg tgagggcgtc
      781 cagctcgctc tcgcgctcga ccagcttcat cgcgcctgga tgatcgtcct ctcgacctcg
      841 ccgccgcggg cgatgaacag ctcactggtc atgccggggg ggaagaggcc gtgggcggcg
      901 acgccggggt ccgcctcgag gcgggcggcg agctccgccg ggtcgccgat cgcacccatg
      961 tagtcgacga tcaggccgcc gtcggggctg cgggggacgt cacgcggctc gaactcgcca
     1021 ccgagccggg ccagggtcga ggccgcgccg aacccgaaca cctcgaccgg caccgggccg
     1081 cgcagaacgt cgaccggctt gctcgagtcg gcgatcacga cgaaccgcgc cgccgccccg
     1141 gcgacgatct tctcgcgcag gtgggcgccg ccgccgccct tgatcagcca gccgtcgggg
     1201 gtgacctcgt cggtgccgtc gatcgcgatg tcgagccgcg ccagctcgtt gaactcctcg
     1261 accgggatct cgagctcgcg cgcctgttcg gaggttgcca ccgaggtggc aacgcagcga
     1321 atctcgccca gcccgcgtcg tgcgatcgcc ggcagcaggt gggcgacggt cgaacccgtg
     1381 cccaggccga tcgtcatccc gctctcgacc agctccgccg cggcctccgc cgccagtcgc
     1441 ttctcatcgt cagtcccggg cacggcggcg aactctattc gctgagttgt ccatacttaa
     1501 ggccgattca cgcgcttgta cgtagtgcaa actgcgcgta cgcaaaaacg acgatccggg
     1561 agaaactcga ctcgatgacc accagcaccg caactcagga ctacaaagtc gccgacatcg
     1621 gactggctga gttcgggcga aaagagattt cgctggccga ggtcgagatg ccgggcctga
     1681 tgcagacgcg cgaggagtcc gccgccgcgc agccgctgaa gggcgcccgg atcaccggct
     1741 cgctccacat gaccgtgcag accgcggtcc tgatcgagac cctggtcgcg ctcggtgccg
     1801 aggtgcgctg ggcatcctgc aacatcttct cgacccagga ccacgccgcc gccgcgatcg
     1861 ccgccgccgg cattcccgtc tacgcctgga agggcgagac cctggaggag tactggtggt
     1921 gcaccgagca ggcgctgctc tggcccgacg gcgagggccc gaacatgatc ctcgacgacg
     1981 gtggcgacgc caccctgctc gtccacaagg gcgtcgagtt cgaggccgcc ggcgccgtgc
     2041 cggacccggc cggtgccgag tccgaggagt tccggatcgt gctcgagacc ctgcagcgct
     2101 cgctcgaggc cgacccgcag cgctggacca agctcgccga cgggatcaag ggcgtcaccg
     2161 aggagacgac caccggcgtg caccggctct accagctggc cgagaccggg gacctgctgt
     2221 tcccggcgat caacgtcaac gactcggtga ccaagtcgaa gttcgacaac ctctacggct
     2281 gccggcactc gctggtcgac gggatcaacc gcgccaccga cgtgatgatc ggcggcaagg
     2341 tcgcggtcgt ctgcggctac ggcgacgtcg gcaagggttg cgcttcttcg ctgcgcggcc
     2401 agggtgcccg cgtgatcgtc accgagatcg acccgatctg cgccctgcag gcggcgatgg
     2461 agggctacga ggtgcgcaag ctcgccgacg tcgtcgagac cggggacatc ttcgtcaccg
     2521 cgacgggcaa caaagacatc atcaccgccg ccgacatggc gcgcatgaag cacaacgcga
     2581 tcgtcggcaa catcggccac ttcgacaacg agatcgacgt cgccggcctc gaagcccacc
     2641 cgggcgtcga gcggatcaac atcaagccgc aggtcgacga gtggcgcttc gccgacggcc
     2701 actcgatcgt gctgctctcc gagggccgcc tgctcaacct cggcaacgcc accggccacc
     2761 cgagcttcgt catgtcgaac tcgttcacca accaggtgat cgcccagatc gccctcttcg
     2821 gcgagagcga gcagtacgcg gcgaaacagg tctacgtgct gcccaagcac ctcgacgaga
     2881 aggtcgcccg cctgcacctg gccgccctcg gcgtcgacct caccgagctc accgccgagc
     2941 agtccgccta cctggggatc ccggtcgagg gcccctacaa gcccgagcac taccgctact
     3001 gagtcaggct ctcgctggct agctggcgga gacggccagc gtcgcctcca tgcctttggc
     3061 ctcgtgtttg tagaggctgc agtagaggcg gtagctgccg ggggagaggg tgagggtggc
     3121 gaaagtgctg ggctgcgcct ggcaggtcca ccagtgcttg aggcgcttta cccgttgttg
     3181 cttaccgtgc cggcgcaccc gcttgacgac ctgcttggag tggcgcttcc aggtgcatcc
     3241 ctcgatcggc gacggcgcag cagtcgcggc gggcatcacg agcggaccgc cggcaatcgc
     3301 tgcgagcgcg aggccgagga cgatcccggc gccgacctgg ccgcggcgga tcgtcgctgc
     3361 cgtgcttatg ccttcaccag gaccggccgc tcgaacgagg aagcgccggg gatgaccgag
     3421 gccgcgtcgt gccccagcca ttcctccagc aacgagcagt acatcgcgcg gaaatcgctg
     3481 gtcacgcgga cgttgtcgtt ctggtcgagc tgcgccaggc cggggaactc gccgaccatc
     3541 cgggaccgat gtcatccaat cgacactgtg agttgacact tccgtgcggc tcaatctggc
     3601 tttggattgg caaagatagt ttgctaaact aattactaaa gctaatcaat ggcaggaaag
     3661 accaaagaca ccactctctt cgatgctgcg gctcagttgc ggctggggat cgtgcgcacg
     3721 gcgcggcgcc tgcggcagga ggcggcgtct gaggcgaccg gcctcacgcc tacctcgact
     3781 gcggcgctgg cgacgatcga gcgacacggg ccgttgacgc ccagcgagct ggctgagctc
     3841 gagcgggtca agcggccgac cgtgacccgc accctgggtt gcctcgagcg ggagggcctg
     3901 atcgagcgca cgcccgatcc ggcggacggt cgcagcacgc tgatcagcgt caaccctgcc
     3961 ggccgcgagc ggctgcggcg gctgcgcggg cgtaagaacg cctacctggc ccgccgcatg
     4021 cgcgagatgc cggccaccga cgtcgaggtc ttggagcggg cggcggagat cctcgagcgg
     4081 atgctggagg atgagcggcg gtgagcgccg ctctgcgccg ctcgttcaac tcgctcgagg
     4141 ttcccaacta ccggcgctac ttcgccggcc agctgatctc cctgtcgggc acctggatgc
     4201 agacggtggc ggcgatctgg ctgatcctga cgctgaccgg cagcggcgtc gcggtcggcc
     4261 tgaccacggc gctg
//
LOCUS       DASRIG010000011         5486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1829996, whole genome shotgun sequence.
ACCESSION   DASRIG010000011 DASRIG010000000
VERSION     DASRIG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829996"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFB52_00190"
     CDS             <1..202
                     /locus_tag="VFB52_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04780.1"
                     /translation="LGEELAAADSIDAGLASFSARRIGRNEFVVNRSAKLSALQMDPT
                     DVDGSAFTAAMGESVGVLAEEF"
     gene            316..1257
                     /locus_tag="VFB52_00195"
     CDS             316..1257
                     /locus_tag="VFB52_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO04781.1"
                     /translation="MFGLLSQLAHAELESPRPEESAAFFTEVLGLYETERDGGSIYLR
                     AWGDPFHHSLVISEGAQPRIAHVGWRTAGAEALEGAAAAIEATGLGEGWQEPVLGHGR
                     AYRFRSPGGNLHEVFWDADRYVAPEAQRSTLPNRPQRFLPVGVGARQIDHITFVTPKI
                     MDDVAFWRDTLGDRFMEFAVDENDEVVFAEVTSNEQAHDIGLVGVAAAPGGQTHHLAY
                     WLDLPSDVHRAADVLRESGHSPEWGPARHGHGENTFLYVREPGGHRIELFSGGYRNYQ
                     PDWEPRKWLANSGGVEMFVNNPMPESMFEAFPAGERR"
     gene            1254..2276
                     /locus_tag="VFB52_00200"
     CDS             1254..2276
                     /locus_tag="VFB52_00200"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZO04782.1"
                     /translation="MRYGKGEAREWAKEHLRGVAGCVAPTVTADYSKLNLEAIRHDVR
                     KEKEDGFAGILLVGESGATDAEMRQFIDVAVEEANGELLTILQAAEPTLAANIDLVQY
                     GERAGVDVVLPSFPTGFYPVTEQEVYDYYTALAGSTSLGMFVFAIHLWNFGRLHPSTF
                     SPALIRRLVDDCPNVVAIKNEIGSPGVAGLSQVFELFNDEVVVTDPFEMNAPAWVKTY
                     GMQFLGTSNYEYAGSVVPRMFDLLHSADDYDEAMDLYWKVQPARQANMKLIGTAVAGT
                     AVVPRMLWKYQGWVNGYNGGPIRSALSGRLSDEQMRSFRASAVACGLGADEEPDAAFF
                     VGRNPA"
     gene            2280..3275
                     /locus_tag="VFB52_00205"
     CDS             2280..3275
                     /locus_tag="VFB52_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZO04783.1"
                     /translation="MTLILDNEDVLAAAEMSAVIAALEDAMRIEAAGGVETVPRVNLP
                     IAPKGFFRVMPAVIDSLDLMGLKVFNTSRSSEVRYLIGLWSIESGELKALVDACHLTA
                     IRTGAVTAMAHRAIAGDRVAEEIGIIGSGLEARTNLEAICCVAEIRRARVFSPNPERR
                     ERFAAEMSARLGIEVLPVATPAEAADCPAVLAATNTGIGSGRIALESEWVRPAGHVDS
                     IGSTMPALRELGAELFGRADLIVLDTLDSIEESGDLIAARESGNWEDSRVRTLASLFA
                     ADAPPPRPSADSLTIFKSVGTALQDLIAADAIFRVAAEQGRGREVDFLYGKEFVA"
     gene            3275..4681
                     /locus_tag="VFB52_00210"
     CDS             3275..4681
                     /locus_tag="VFB52_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO04784.1"
                     /translation="MAIESVNPATNETLRSFEAYAPAQVDSLISTAHAAFAEWSRRPL
                     EQRTELVRGAGELLRSNRDELAELATLEMGKVFKEAAFEVEKAAGFCDYYADRAARFL
                     APRQIEGGQAENWVRYEPLGAILAVMPWNFPYVQVFRFAPAMLVAGNVVLLKHASNVP
                     QCALRIERLFAEAGLPAGAFTTLLIGSGEVARVLADERVRAVTLTGSEQAGSQVAAVA
                     GRELKHSVMELGGSDPFIVLADADVADAARKGARARTSNTGQACINAKRFIVLEEVAE
                     EFEAALIDEVARLRVGDPMSPETDIGPLAHQGAVEDLRRQVASSVAAGAAVAVGSCEV
                     DRPGAYVEPMVITGVTPEMSVFREETFGPVAAVTRVPDERTAIALANDSAYGLGASIW
                     TADLERAQELAGELEAGMVFVNEIVVSDARLPFGGSKRSGYGRELGEWGIHEFTQIKS
                     IAVSAPGEGADAATLVAE"
     gene            4684..>5486
                     /locus_tag="VFB52_00215"
     CDS             4684..>5486
                     /locus_tag="VFB52_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO04785.1"
                     /translation="MDEVLLTTLSETASDLASLRDVDEILTAICRRSRALLGADAAYI
                     ALRDEQRGDTYVRATDGVVSEPFRRLRLPVSAGVGGLVAAEGIPVATSDYLEDARFVH
                     TAELDRCVREEGLRAIVGVPLRRGEEVSGVLMGGFRAPRRLEPTEVGWFQALAAHAAI
                     AIHDRRAEAAASLHEELARLLVAGGGPAELLEAIARSTGAELELLDADGARLAGGGAP
                     WRGCEAGEIPVLAGGERLGTLRWSATEPGVGADRVELLERGCTILAGQLL"
BASE COUNT          788 a         1939 c         1998 g          761 t
ORIGIN      
        1 gctgggcgag gagctggcgg cggcggactc gatcgacgcc gggctcgcca gcttctccgc
       61 gcgccgcatc ggccgcaacg agttcgtcgt caaccgctcg gccaagctga gcgcgctgca
      121 gatggatccg accgacgtcg acggcagcgc cttcaccgcc gcgatggggg aatcggtagg
      181 cgtgctggcg gaagagttct agggagtttg tatgcaaact cgtgtttaca tccaatttga
      241 tagggtcgct tcgaaccctg aaagtcaacg ccgcgcaccg ctcccggcgg tcgccgatcc
      301 acggaggaat gccctgtgtt tggtctgctg tcacagctag ctcacgccga gctggagagc
      361 ccgcgccccg aggagtcggc ggccttcttc accgaggttc tcggcctcta tgagaccgag
      421 cgggacgggg gctcgatcta cctgcgggcc tggggcgacc ccttccacca cagcctcgtg
      481 atcagcgagg gcgcgcagcc gcggatcgcc cacgtcggct ggcgcaccgc gggtgccgag
      541 gcgctcgagg gggcggccgc ggcgatcgag gcgaccgggc tcggcgaggg ctggcaggag
      601 ccggtgctcg gccacggccg cgcctaccgc ttccgctcgc ccgggggcaa cctgcacgag
      661 gtcttctggg acgccgaccg ctacgtcgcc cccgaggcgc agcgctcgac cctgcccaac
      721 cgcccccagc gcttcctccc ggtcggggtc ggcgcacggc agatcgacca catcaccttc
      781 gtgacgccga agatcatgga cgacgtcgcc ttctggcgcg acaccctcgg cgaccgcttc
      841 atggagttcg ccgtcgacga gaacgacgag gtcgtgttcg ccgaggtgac cagcaacgag
      901 caggcccacg acatcggcct ggtcggggtc gccgccgccc ccggcggtca gacccaccac
      961 ctcgcctact ggctcgacct gccctccgat gtccaccgcg ccgccgacgt cctgagggag
     1021 tccggacact cgcccgagtg gggaccggcc cggcacggtc acggcgaaaa caccttcctc
     1081 tacgtgcgcg agcccggcgg ccaccggatc gagctgttct ccggcggcta ccgcaattac
     1141 cagcccgact gggagccgcg caagtggctg gccaactccg gcggggtcga gatgttcgtc
     1201 aacaacccga tgcccgagtc gatgttcgag gccttcccgg ccggtgagcg ccgatgaggt
     1261 acggcaaggg agaggcccgg gagtgggcca aggagcacct gcgcggggtc gccggctgcg
     1321 tcgccccgac ggtcaccgcc gactactcga agctgaacct ggaggcgatc cgccacgacg
     1381 tccgcaagga gaaggaagac gggttcgccg gcatcctcct ggtcggggag agcggcgcca
     1441 ccgacgccga gatgcgtcag ttcatcgacg tcgcggtcga ggaggcgaac ggcgagctgc
     1501 tgacgatcct gcaggcggcg gagccgaccc tggctgccaa catcgacctc gtccagtacg
     1561 gggagcgggc cggcgtcgac gtcgtcctgc cctccttccc gaccggcttc tacccggtca
     1621 ccgagcagga ggtctacgac tactacacgg cgctggccgg ctcgacctcg ctcggcatgt
     1681 tcgtcttcgc gatccacctc tggaacttcg gacggctgca cccgagcacc ttctcgccgg
     1741 ccctgatccg gcgcctggtc gacgactgcc cgaacgtggt cgcgatcaag aacgagatcg
     1801 gctcgcccgg cgtcgccggg ctctcgcagg tcttcgagct gttcaacgac gaggtcgtgg
     1861 tcaccgaccc gttcgagatg aacgccccgg cctgggtgaa gacctacggg atgcagttcc
     1921 tcggcacctc caactacgag tacgccggct cggtcgtgcc gcggatgttc gacctcctgc
     1981 acagcgccga cgactacgac gaggcgatgg acctctactg gaaggtgcag ccggctcgcc
     2041 aggcgaacat gaagctgatc ggcaccgccg tcgccggcac cgcggtcgtg ccgcggatgc
     2101 tctggaaata ccagggctgg gtcaacggct acaacggcgg cccgatccgc agcgcgctca
     2161 gcggccggct cagcgacgaa cagatgcgct ccttccgggc cagcgccgtg gcctgcggcc
     2221 tgggcgcgga cgaggagccg gacgcggcgt tcttcgtcgg gcgcaatccc gcctgagcga
     2281 tgaccctgat tctcgacaac gaggacgtgc tcgccgcggc cgagatgagc gccgtgatcg
     2341 cggcgctcga ggacgcgatg cggatcgagg ccgcgggcgg cgtggagacc gtgccccggg
     2401 tcaacttgcc gatcgccccg aagggcttct tccgggtgat gccggcggtg atcgactcgc
     2461 tcgacctgat ggggctgaag gtcttcaaca ccagccgcag cagcgaggtc cgctacctga
     2521 tcggcctctg gtcgatcgag tcgggtgagc tgaaagcgct ggtcgacgcc tgccatctga
     2581 ccgcgatccg gaccggggcg gtgaccgcga tggcccaccg ggcgatcgcc ggcgaccggg
     2641 tggccgagga gatcgggatc atcggctccg gcctcgaggc gcgcaccaac ctggaggcga
     2701 tctgctgcgt cgcggagatc cggcgggcca gggtgttcag cccgaacccg gagcgccgcg
     2761 agcgcttcgc cgccgagatg tcggcgcggc tggggatcga ggtcctcccg gtcgccactc
     2821 cggcggaggc ggccgactgc cccgcggtgc tcgccgccac caacaccggg atcggcagcg
     2881 gccggatcgc gctcgagtcc gagtgggtgc gcccggccgg gcacgtcgac tcgatcggct
     2941 cgacgatgcc ggcgctgcgt gagctcggcg ccgagctgtt cgggcgcgcc gacctgatcg
     3001 tgctcgacac gctcgactcg atcgaggagt cgggcgacct gatcgccgcc cgcgagagcg
     3061 gcaactggga ggactcccgg gtgcggaccc tggcctcgct gttcgccgcc gatgcgccgc
     3121 cgccacggcc ctccgcggac agcctcacga tcttcaagtc ggtcggcacg gcgctccagg
     3181 acctgatcgc ggccgacgcg atcttccggg tcgcggcgga gcagggccgc ggccgcgaag
     3241 tcgacttcct ctacggcaaa gagttcgtcg cctgatggcg atcgagtcgg tcaacccggc
     3301 gaccaacgag acgctgcgca gcttcgaggc gtacgcgccg gcccaggtcg acagcctgat
     3361 ctccaccgcg cacgccgcgt tcgcggagtg gtcgcggcgc ccgctcgagc agcgcacgga
     3421 gctggtcaga ggggccggcg aactgttgcg gtccaaccgc gacgagctgg ccgagctggc
     3481 gaccctggag atgggcaagg tcttcaagga ggcggccttc gaggtcgaga aggcggcggg
     3541 gttctgcgac tactacgccg accgcgccgc ccgcttcctg gcgccgcgcc agatcgaggg
     3601 cgggcaggcg gagaactggg tccgctacga gccgctcggc gcgatcctcg ccgtgatgcc
     3661 ctggaacttc ccctacgtcc aggtcttccg cttcgccccc gcgatgctcg tggccgggaa
     3721 tgtcgttctg ctgaagcacg cctcgaacgt gccccagtgc gcgctgcgga tcgagcggct
     3781 gttcgccgag gcgggactgc ccgccggggc cttcacgacg ctgctgatcg ggtcggggga
     3841 ggtcgcgcgg gtgctcgccg acgagcgggt gcgggcggtg accctgaccg ggagcgagca
     3901 ggccggcagc caggtcgccg cggtcgccgg acgcgagctg aagcactcgg tgatggagct
     3961 cggcggctcg gacccgttca tcgtcctcgc cgacgccgac gtagccgacg ccgcccgcaa
     4021 gggcgcccgg gcgcggacca gcaacacggg ccaggcctgc atcaacgcga agcgcttcat
     4081 cgtcctggag gaggtggccg aggagttcga ggcggcgctg atcgacgagg tcgcgcgcct
     4141 gcgggtcggc gacccgatga gccccgagac cgacatcggg ccgctcgccc accagggcgc
     4201 ggtcgaggac ctgcgccgcc aggtcgcctc ctcggtcgcc gcgggcgccg cggtcgcggt
     4261 cggcagctgc gaggtcgatc ggcccggggc ttacgtcgag ccgatggtga tcaccggggt
     4321 gacgccggag atgagcgtct tccgcgagga gaccttcggc ccggtcgcgg cggtcacccg
     4381 cgttcccgac gagcggaccg cgatcgcgct cgccaacgac agcgcctacg ggctcggggc
     4441 ctcgatctgg accgccgacc tggagcgggc ccaggagctg gccggcgagc tggaggcggg
     4501 gatggtcttc gtcaacgaga tcgtcgtctc cgacgcgagg ctgcccttcg gcggcagcaa
     4561 gcgcagcggc tacggccgcg agctcggcga gtgggggatc cacgagttca cccagatcaa
     4621 gtcgatcgcg gtcagcgccc ccggcgaagg ggccgacgcg gcgacgctcg tcgccgagta
     4681 gcggtggacg aggtcctgct caccaccctg agcgagaccg cctccgacct cgccagcctg
     4741 cgtgacgtcg acgagatcct gaccgcgatc tgccgccgct cgcgggcgct gctcggcgcc
     4801 gacgccgcct acatcgcgct gcgcgacgag cagcgcggcg acacctacgt gcgcgccacc
     4861 gacggggtcg tctccgagcc cttccggcgg ctgcgcctgc cggtcagcgc cggggtcggc
     4921 ggcctcgtcg ccgccgaagg cattccggtc gcgaccagcg actacctcga ggacgcccgc
     4981 ttcgtccaca ccgccgagct cgaccgctgc gtgcgtgagg agggcctgcg ggcgatcgtc
     5041 ggcgtccccc tgcgccgtgg agaggaggtc agcggcgtgc tgatgggcgg cttccgcgcg
     5101 ccgcgccggc tcgagcccac cgaggtgggc tggttccagg cgctggcggc gcacgccgcg
     5161 atcgcgatcc acgaccggcg cgccgaggcg gccgcgagcc tgcacgagga gctcgcgcgg
     5221 ctgttggtcg cgggcggcgg cccggccgag ctgctggagg cgatcgctcg cagcaccggg
     5281 gcggagctgg agctgctcga cgccgacggg gccaggctgg cgggcggcgg cgcgccctgg
     5341 cggggatgcg aggcagggga gatcccggtt ctcgccgggg gcgagcggct cggcacgctg
     5401 cgctggagcg ccaccgagcc cggcgtcggc gccgaccggg tcgagctgct ggagcggggc
     5461 tgcacgatcc tggccggcca gctgct
//
LOCUS       DASRIG010000012         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2354132, whole genome shotgun sequence.
ACCESSION   DASRIG010000012 DASRIG010000000
VERSION     DASRIG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354132"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /gene="purB"
                     /locus_tag="VFB52_00220"
     CDS             <1..1282
                     /gene="purB"
                     /locus_tag="VFB52_00220"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZO04786.1"
                     /translation="IARYTRPEIGAVWTQQAKLESWLEVELAATEAWAEEGVVPREAA
                     ETARANAAFTVEAVDEREKVTDHDVAAFVDVVAASVGEHGRWIHYGLTSSDVLDTALA
                     LQLRRAGEIVVAGARAYRDALVERALEQRDTLCVGRTHGVHAEPTTFGLRLAGFAFEA
                     DRNLTRLKEAFAHLAFGKLSGAVGTYASIPPAVEARVMERVGLGTEDVATQVVPRDRH
                     AVLLSAIAVAGAGLERFATEVRGLQKTELREVEEPFAAGQKGSSAMPHKRNPIRTERI
                     TGLARVLRGYAQTGLENVALWHERDISHSGAERVILPDATILIDYMQDLGTKVASGMT
                     INAERMRENLELTYGALFSQRALTALVESGMTRDDAYRIVQENAQRAWDTRTEFRSLL
                     AEAAPDLDLDAVFDYNAYLTHLPEIFERLEALQD"
     gene            complement(1334..1678)
                     /locus_tag="VFB52_00225"
     CDS             complement(1334..1678)
                     /locus_tag="VFB52_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04787.1"
                     /translation="MKRILGLLLCLAALLALPAAAQAAPPTTTIYGANWGDDDSKVEV
                     EFLSEGATTIECSVDEIIAWYECGSEAYEWNGKLRVWFEIWPYYTQGHCFYIRGTNED
                     GTEAEPPCYYRE"
     gene            2028..>2660
                     /locus_tag="VFB52_00230"
     CDS             2028..>2660
                     /locus_tag="VFB52_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO04788.1"
                     /translation="MPRSWYRKLFPAVLAIGAFLGLIPAAASAADPPDPLDRGSYTVE
                     RINPFTAGLATLQEPNGSGGAPGTGSNASVTLQIRGDLYRPSDYSGKSPVIVLVHGNH
                     GSCDDNGDSSLNPPTCDVYKRNDSGYAYLGENLASWGYTVVSLDQDQMMMRQDGFARG
                     MHQRRLLIMALLDALYEANENGLPVNENSNIGDLLKGKLDMTRIGMMGHSR"
BASE COUNT          462 a          915 c          874 g          409 t
ORIGIN      
        1 gatcgctcgc tacacccgcc ccgagatcgg ggcggtctgg acccagcagg caaagctcga
       61 gagctggctc gaggtcgagc tggcggcgac cgaggcctgg gccgaggagg gcgtggtccc
      121 gcgcgaggcg gcggagacgg ctcgcgccaa cgccgccttc acggtcgagg cggtcgacga
      181 gcgcgagaag gtgaccgacc acgacgtcgc cgccttcgtc gacgtggtcg ccgcctcggt
      241 cggtgagcac gggcgctgga tccactacgg gctgacctcc tccgacgtgc tcgacacggc
      301 gctggcgctg cagctgcggc gagccggcga gatcgtcgtc gccggggcgc gcgcctaccg
      361 cgacgccctg gtcgagcggg cgctggagca gcgcgacacg ctctgcgtcg gccgaaccca
      421 tggcgtgcac gcggagccga ccaccttcgg gctgcggctc gccggcttcg ccttcgaggc
      481 cgaccgcaac ctcacccgcc tcaaggaggc cttcgcccac ctcgccttcg gcaagctctc
      541 cggcgcggtc ggcacctacg cctcgatccc gcccgcggtc gaggcgcggg tgatggagag
      601 ggttgggctc ggcaccgagg acgtcgccac ccaggtcgtg ccacgcgacc gtcacgcggt
      661 cctgctctcg gcgatcgcgg tcgccggcgc cggcctcgag cgcttcgcga ccgaggtccg
      721 cggcctgcag aaaaccgagc tgcgcgaggt cgaagagccg ttcgccgccg gccagaaggg
      781 ctcctcggcg atgccgcaca agcgcaaccc gatccgcacc gagcggatca cggggctcgc
      841 ccgggtgctg cgcggctacg cccagaccgg cctagagaac gtcgccctct ggcacgagcg
      901 cgacatctcc cactccggcg ccgagcgggt gatcctgccc gacgcgacga tcctgatcga
      961 ctacatgcag gacctcggca ccaaggtggc gagtgggatg acgatcaacg ccgagcgcat
     1021 gcgcgagaac ctcgagctca cctacggcgc cctgttcagc cagcgggcgc tgaccgcgct
     1081 ggtcgagtcg gggatgaccc gcgacgacgc ctaccggatc gtccaggaga acgcccagcg
     1141 cgcctgggac acccgcaccg agttccgttc gctgctggcc gaggcagcgc cggacttgga
     1201 cctcgacgcg gtcttcgact acaacgccta cctcacccac ctgcccgaga tcttcgagcg
     1261 gctggaagcg ctgcaggact gacgcgaggg gaccggcgga cccggccccc tcgcacgagc
     1321 ggatcagccc tcgctattcc cggtagtagc aggggggttc ggcctcggtt ccgtcttcgt
     1381 tggtgccgcg gatgtagaag cagtgaccct gcgtgtagta gggccagatt tcgaaccaga
     1441 cgcgcagttt gccgttccat tcgtaggctt cgctgccgca ctcgtaccag gcgatgattt
     1501 cgtcgaccga gcattcgatc gtcgtcgccc cttcgctgag gaattcgacc tcgaccttgg
     1561 agtcgtcgtc accccaattg gcgccgtaga tcgtggtcgt cgggggtgcc gcttgggcgg
     1621 cggcggggag cgccagcagg gcagcgagac agagaagtag gccgagtatt cgcttcactg
     1681 agtttccttt cgacgtctgc ccggaagggg cagagactgg tttcacttaa gaaattcaga
     1741 ttggcaagca aagctgaact atgtcaaggg gtccccacca tttgacggga aagtctggac
     1801 cgcgggtcca cttgtaacag tgttacttgg accgtcggtc caggacctct ccaagtagtg
     1861 ctgaccccca cctaagacgc ttgtacaccg ggacagcatg gggtctcaaa gactgtccag
     1921 atggggatgt ttgcgcccta cccccagccc tagattcgcc ggtctgcgcg gtgctgccgt
     1981 cccagcccgg gcatgcccca gataccccta aatcccggga gatgcaaatg ccacgaagtt
     2041 ggtacagaaa gctgttcccc gcggtcctag caattggagc cttcctcggg ctgatccccg
     2101 ccgcagcgtc ggcggcggac ccgcccgatc cgctcgaccg cgggtcctac acggtggaac
     2161 ggatcaaccc gttcaccgcc ggcctggcga cgctgcagga accaaacggt tccggcggag
     2221 cgcccgggac cgggtccaac gcctcggtga cgctgcagat tcgcggtgac ctctaccgac
     2281 cctcggacta ctcgggcaaa tcgccggtga tcgtgctggt gcacggaaac cacgggagct
     2341 gcgacgacaa cggcgatagc agcctcaacc cgccgacctg cgacgtctac aagcgcaacg
     2401 actccggcta cgcctatctc ggcgaaaacc tcgccagctg gggctacacc gtcgtctcgc
     2461 tcgaccaaga ccagatgatg atgcgccagg acggcttcgc gcgcggcatg catcagcgaa
     2521 ggctgctgat catggcgctg ctcgacgcgc tctacgaggc caacgagaac gggctcccgg
     2581 tgaacgaaaa ctcgaacatc ggcgacctgc tcaaaggcaa gctggacatg acccggatcg
     2641 ggatgatggg gcactcgcgc
//
LOCUS       DASRIG010000013         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_267380, whole genome shotgun sequence.
ACCESSION   DASRIG010000013 DASRIG010000000
VERSION     DASRIG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267380"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFB52_00235"
     CDS             <1..241
                     /locus_tag="VFB52_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04789.1"
                     /translation="ALAMQAGTLDGRDDTVFRWLPLLVDQIGWNEVVAIMEEATEKVL
                     AAHLRSQDRIEHAEEAVSAVIGMAVFETAGSQQAA"
     gene            complement(550..2166)
                     /gene="guaA"
                     /locus_tag="VFB52_00240"
     CDS             complement(550..2166)
                     /gene="guaA"
                     /locus_tag="VFB52_00240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZO04790.1"
                     /translation="MTDPVLARSDDDLVVEPGEELPADDVRAVEEVLVLDFGGQYSQL
                     IARRIRECGVFAELLPSTTPIEKIRERAPKALVLSGGPASVYEAEAPPFPTQLLDLEI
                     PMLGICYGMQAMMLALGGKVESAESGEFGRTDLTLTNDGGRLLGGLPEEQTCWMSHRD
                     AVFQAPEGFVALASSPSSPVAAAELPDRGLYGIQFHPEVVHTPYGTEILTRFLRDVAG
                     CVEQWSPKSVIAEQVEKIQAQVGEGKVICGLSGGVDSSVAAMLVHKAVGDKLTCVFVD
                     HGLMRMNEAEQVVETFSEFDIPLVHVEAEERFLAKLKGHDDPETKRKIIGEEFIRVFE
                     DEAAKLEDVSFLVQGTLYSDVIESGGSDHAATIKSHHNVGGLPDDLEFDLVEPLRMLF
                     KDEVRAVGAELGLPERMVWRQPFPGPGLGIRIVGGEVNKERLDILRAADAILQEEVRA
                     AELYRQLWQSFCVLPVVRSVGVQGDGRTYAYPIVIRAVTSDDAMTADWARLPYDLLER
                     VSNRIINEIRDVNRVALDISSKPPATIEWE"
     gene            complement(2159..3364)
                     /locus_tag="VFB52_00245"
     CDS             complement(2159..3364)
                     /locus_tag="VFB52_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HZO04791.1"
                     /translation="MEIEIGRGKKGRRAYGFDDIAIVPSRRTRDPDDIDITWTLGPYR
                     FDMPLLASAMDGVVSPETAVLTNELGGLGVLNLEGIWTRFENAEEKLEAIAAAPKHLA
                     TRMMQEIYAEPVKEELISQRVAEIKARGAVVCGSLTPQKVRDHYEAAVDAGLDILVIQ
                     GTVISAEHVSTTVEPLNLKEFIAEVPVPTVVGGCASYSTGLHLMRTGAVGVLVGVGPG
                     AACTTRGVLGIGVPQATAIADVAAARAQHMLETGEYVNVIADGGMRTGGDISKAIACG
                     ADSVMIGSPLARAKEAPGRGFHWGMATFHSSLPRGTRVSTKQDGTMEEILLGPAQEND
                     GTFNLMGALKTSMATCGYEDIRSFQRAEVMVAPALMSEGKKLQNEQSVGMGSNGRAAV
                     SAGVAVSDD"
     gene            complement(3402..4358)
                     /locus_tag="VFB52_00250"
     CDS             complement(3402..4358)
                     /locus_tag="VFB52_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HZO04792.1"
                     /translation="MILTVTLNAAIDRTVAVPNFRLARRHRAVESRTVAGGKGINVAR
                     ALTLLGRPVIATGFVGGPTGTRVLEELRQESVLSDFTLIAAETRINLAVIDPTSGEQT
                     EINERGPAVSPEEVKKLFERIGYLANGAKICVLAGSLPPGAGDDLYARLINDLSRRGV
                     VVVLDAEGEAMLEGVRAGASMVTPNEREAEELVGQEFSDRGDLAAGLHELVRLGASEA
                     AITRPEGCVAAVGEGSERRLLEVHTEPLEPVSTVGSGDAFLAGYVAARYDGRSAEDCL
                     AYGVACGAESTQHFGAGTVDRNQVERLLGEVAVHDLEVPAEV"
     gene            complement(4393..5691)
                     /locus_tag="VFB52_00255"
     CDS             complement(4393..5691)
                     /locus_tag="VFB52_00255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZO04793.1"
                     /translation="MRTKFVLLLALLACALLAAPAGAAEKQPDSQGSQSGNTQGKQAE
                     KKQQPKKKKKPKPKPKTPAEKLGIYAKSWAVIDARTGEVLLSQAAKKRLPIASTTKLM
                     TAYVAMKEMPLDKVVRAAPYDAEFGESLLEMEAGEKISVRDLIYGLILKSGNDAAHTL
                     AVRVAGSTKRFVAQMNRYAAALGLADTHYANPIGLDAKGNYSSARDLLTLTQRLLRMP
                     EFAKISNTESVVLKSTNPPRRIDTINELLLMEPWVTGVKTGHTWGAGYVLVGAGERKG
                     VELISVAIAAPTDETRYSDNLALLEYGFSQYQKKKPVKRGEQFAEAEIRYTGGSLPLR
                     AEHGIKVGIRKGQDLTFKVVAPEEVEGPIRRGAKLGRVKVYVDGMKAGAVPLVAARAI
                     SEASDFDRARDFVENNWIPIAVALFVILVGGALLVRRLSR"
     gene            complement(5691..6008)
                     /locus_tag="VFB52_00260"
     CDS             complement(5691..6008)
                     /locus_tag="VFB52_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZO04794.1"
                     /translation="MKKRTSVFARLFALLAIIAALAAVYVAVSGALDEDSSKNGKQSN
                     QHAKTEKEEKPKITAATYEVEEGDTLTRISHLTGVKVTRIIELNPETDPQGLVIGEVL
                     KLR"
     gene            complement(6063..>6386)
                     /locus_tag="VFB52_00265"
     CDS             complement(6063..>6386)
                     /locus_tag="VFB52_00265"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HZO04795.1"
                     /translation="PLHERLAEIVDGLAPRLAGESGLSGMGAVVGATEPQHIGRLREL
                     MPRATFLIPGVGAQGGQPELLGPAFSAGRSAALVAASRGIAGAEDPAAAAEELRETVW
                     KISTA"
BASE COUNT          933 a         2153 c         2226 g         1074 t
ORIGIN      
        1 cgcactggcg atgcaggcgg gcaccctcga cggccgcgac gacaccgtgt tccggtggct
       61 gccgctgctg gtcgaccaga tcggctggaa cgaggtcgtg gcgatcatgg aagaggcaac
      121 cgagaaggtc ctcgcggcgc acctgcgcag ccaggaccgg atcgagcatg ccgaggaagc
      181 ggtctcggcc gtgatcggga tggcggtctt cgagaccgcc ggctcccagc aagcggccta
      241 gacgacggtg cgggggcggg cgggcgccct caccgtcgac tcatctctcg gcccaaggat
      301 cgggccacta gctttgacag agggctcggg cgtctgctcc tgtcgcccgg agccggcggc
      361 agccgccagc gctcaagcga gctgacggat cttcggggcg atttgggagg tggggtcggg
      421 gtggccctga agcgtcttgt gtttttttgc ttcagcgctg cgaggcgtct agccgccgct
      481 caatccccaa aaaaactaag cggaagggcc accccgaccc caccaggcaa acctctccga
      541 aacggccgtc tactcccact cgatggtcgc cgggggcttc gagctgatgt cgagagccac
      601 ccgattcacg tcgcggatct cgttgatgat ccgattagag acgcgctcca ggaggtcgta
      661 ggggaggcgg gcccagtcgg cggtcatcgc gtcgtcggag gtgacggcgc ggatcacgat
      721 cgggtaggcg taggtgcggc cgtcgccctg gacgccgacc gagcggacca cgggcaggac
      781 gcagaaggac tgccagagct ggcggtagag ctcggcggcg cggacctcct cctggaggat
      841 cgcgtcggcg gcgcggagga tgtcgaggcg ctctttgttg acctcgccgc cgacgatccg
      901 gatgccgagg cccgggcccg ggaagggctg gcgccagacc atgcgctcgg gcaggccgag
      961 ctcggcgccg accgcgcgca cctcgtcctt gaacagcatc cgcagcggct cgaccagatc
     1021 gaactcgagg tcgtcgggga gcccgccgac gttgtggtgg gacttgatcg tcgcggcgtg
     1081 gtcggagcca ccggactcga tcacgtcgga gtagagcgtg ccctggacca ggaaagagac
     1141 gtcttcgagc ttcgccgcct cgtcctcgaa cacgcggatg aactcctcgc cgatgatctt
     1201 gcgcttggtc tccgggtcgt catgcccttt gagcttggcc aggaagcgct cctcggcttc
     1261 gacgtggacc agcgggatgt cgaactcgct gaacgtctcg accacctgct cggcctcgtt
     1321 catccgcatc aggccgtggt cgacgaagac gcaggtgagc ttgtcgccga ccgccttgtg
     1381 gaccagcatc gccgcgaccg aggagtcgac accgccggag agaccacaga tcaccttgcc
     1441 ctcccccacc tgggcctgga tcttctcgac ctgttcggcg atcaccgact tcggcgacca
     1501 ctgctcgacg caaccggcga cgtcgcgcag gaagcgggtg aggatctcgg tgccgtaggg
     1561 cgtgtgcacg acctcggggt ggaactggat cccgtagagg ccacggtcgg gcagctccgc
     1621 cgccgcgacc ggcgagctcg ggctggaggc aagcgcgacg aacccctccg gcgcctggaa
     1681 gacggcgtcg cggtggctca tccagcaggt ctgctcctcg ggtaggccgc cgagcaggcg
     1741 gccgccgtcg ttggtcagcg tcaggtcggt gcgcccgaac tcgcccgact cggccgactc
     1801 gaccttgccg ccgagcgcca gcatcatcgc ctgcatcccg taacagatcc ccagcatcgg
     1861 gatctccagg tcgagcagct gggtcgggaa cggcggcgct tcggcctcgt agaccgaggc
     1921 cggcccgccg gagagcacca gcgccttcgg cgcccgctcg cggatcttct cgatcggcgt
     1981 ggtcgagggc aggagctcgg cgaagacgcc gcattcgcgg atccggcgcg cgatcagctg
     2041 cgagtactgg ccgccgaagt cgaggaccag cacctcctcg acggcccgta cgtcgtccgc
     2101 aggcagctcc tcgcccggct cgacgaccag gtcgtcgtcg gaccgggcga gaaccggatc
     2161 agtcatccga gactgcgaca cctgccgata cggcggcgcg accgttggag cccatgccga
     2221 cggactgctc gttctggagc ttcttgccct cgctcatcag cgccggcgcg accatcacct
     2281 cggcccgctg gaaggaacgg atgtcctcgt agccgcaggt ggccatcgag gttttcagcg
     2341 cccccatcag gttgaaggtg ccgtcgttct cctgcgccgg gccgagcagg atctcctcca
     2401 tcgtcccgtc ctgcttggtc gagacgcggg tgccgcgcgg cagcgaggag tggaacgtcg
     2461 ccatccccca gtggaagccg cgaccggggg cctccttggc gcgggccagg ggcgagccga
     2521 tcatcaccga gtcggcaccg caggcaatcg ccttggagat gtcgccgccg gtgcgcatgc
     2581 cgccgtcggc gatcacgttc acgtactcac cggtctccag catgtgctgg gcgcgggcgg
     2641 ccgccacgtc ggcgatcgcg gtcgcctgcg ggacgccgat accgagcacg ccgcgggtcg
     2701 tgcaggcggc gccggggccg accccgacga gcacgccgac cgcgccggtc cgcatcaggt
     2761 gcagcccggt cgagtaggag gcacagccgc cgaccacggt cgggaccggg acctcggcga
     2821 tgaactcctt caggttcagc ggctcgaccg tggtcgagac gtgttcagcg gagatcacgg
     2881 ttccctggat caccaggatg tcgagaccgg cgtcgaccgc cgcctcgtag tgatcgcgca
     2941 ctttctgcgg ggtcagcgag ccgcagacga ccgcgccgcg agccttgatt tcggcgaccc
     3001 gctgggagat cagctcctcc ttgaccggct ccgcgtagat ctcctgcatc atccgcgtcg
     3061 ccaggtgctt cggcgcggcg gcgatcgcct cgagcttctc ctcagcgttc tcgaagcggg
     3121 tccagatgcc ctccaggttg aggactccga gcccaccgag ctcgttggtc agaaccgcgg
     3181 tctccgggct gaccaccccg tccatcgcgg acgcgagcag gggcatgtcg aagcgatagg
     3241 gaccgagggt ccaggtgatg tcgatgtcgt cagggtcgcg ggtacgacgc gaaggaacga
     3301 tggcgatgtc gtcgaatccg tatgcgcgcc gccccttctt gccgcgaccg atttcgattt
     3361 ccaagttagt cctccagggc tggcgtggtg aatcagtcag cctacacttc ggcggggacc
     3421 tccagatcgt ggaccgcgac ctcgccgagc agtcgctcga cctgattgcg atcgacggtg
     3481 ccggcgccga agtgctgggt cgactcggcc ccgcaggcga ccccgtaggc gaggcagtcc
     3541 tcggccgagc ggccgtcgta gcgggcagcg acgtagccgg ccaggaaggc gtccccggag
     3601 ccgaccgtgg agaccggctc cagcggctcg gtgtggacct cgagcaggcg acgctcggag
     3661 ccctcgccga cggcggcgac acagccctcg gggcgcgtga tcgccgcctc ggaggcgccc
     3721 aggcggacca gctcgtgcag gccggcggcg aggtcgccac ggtcggagaa ctcctggccg
     3781 accagctcct cggcctcgcg ctcgttcggg gtcaccatcg aggccccggc gcgcacgcct
     3841 tcgagcatcg cctccccctc ggcgtcgagc acgacgacga cgccgcgccg gctcagatcg
     3901 ttgatcaggc gggcgtagag gtcgtcgccg gcgcccggcg gcagcgagcc ggcgagcacg
     3961 cagatcttcg ctccgttggc cagatagccg atccgctcga acagcttctt gacctcctcg
     4021 gggctgaccg cggggccgcg ctcgttgatc tcggtctgtt cgccggaggt cgggtcgatc
     4081 accgcgaggt tgatccgcgt ctccgccgcg atcagggtga agtccgagag caccgactcc
     4141 tggcgcagct cttccagcac ccgcgtcccg gtcgggccgc cgacgaagcc ggtggcgatc
     4201 accggccggc cgagcagggt cagcgcccgc gccacgttga tccccttgcc gccggccacg
     4261 gtgcggctct ccaccgcgcg gtggcgccgc gcgaggcgga agttggggac ggcgacggtg
     4321 cgatcgatcg cggcgttgag ggtgacggtg agaatcactg cgggtccacc tcggtgcgtg
     4381 ctccttggtc ggtcagcggg acagccggcg taccagcaaa gccccgccta ccagtatcac
     4441 gaacaacgca accgcgatgg gaatccagtt gttttcgacg aagtcgcggg cgcggtcgaa
     4501 atcgctcgct tcggagatcg cgcgggcggc caccagcggc accgcaccgg ccttcatccc
     4561 gtcgacgtag accttgacgc ggccgagctt ggcgccacgg cggatcggtc cctcgacctc
     4621 ctcgggagcg acgactttga acgtcagatc ctggcctttt ctgatcccga ccttgatccc
     4681 gtgctcggcg cgcaggggca ggctgccgcc ggtgtagcgg atctccgctt cggcgaactg
     4741 ctctcccctc tttacgggct ttttcttctg gtactgggag aagccgtatt cgagcagcgc
     4801 caggttgtcg gagtagcgcg tttcgtcggt cggggccgcg atcgcgaccg agatcagctc
     4861 gacgcccttc ctctcgccgg cgccgacgag cacgtagccg gcgccccagg tgtggccggt
     4921 cttcacgccg gtcacccacg gctccatcag cagcagttcg ttgatcgtgt cgatccggcg
     4981 cgggggattg gtgctcttca gcacgacgct ctcggtgttg gagatcttcg cgaattccgg
     5041 catccgcaag aggcgctggg tcagggtcag gaggtcgcgg gcgctcgagt agttgccctt
     5101 cgcgtcgagc ccgatcgggt tcgcgtagtg ggtgtcggcg aggccgaggg cggccgcata
     5161 gcggttcatc tgggcgacga agcgcttggt gctgccggcg acgcggaccg ccagcgtgtg
     5221 ggcggcgtcg ttgccgctct tcaagatcag gccgtagatc aggtcgcgca cgctgatctt
     5281 ttcgccggct tccatttcca gcagcgactc gccgaattcg gcgtcgtagg gcgccgcgcg
     5341 cacgaccttg tcgagcggca tctctttcat cgccacgtag gcggtcatca gcttggtcgt
     5401 gctggcgatc ggcagccgct tcttcgctgc ctgcgagagc aggacttcgc cggtgcgggc
     5461 gtcgatcacg gcccaggatt tcgcgtagat gccgagtttt tccgccgggg ttttcggctt
     5521 tggcttcggc ttctttttct tcttcggctg ctgctttttc tcggcctgct tgccctgggt
     5581 gttgccgctc tgtgagccct gggagtccgg ctgcttttcc gccgcgccgg cgggcgcagc
     5641 gagcagcgca caggcgagca gagcgagcag caggacgaac ttggtgcgca ctagcgcagt
     5701 ttcaggactt cgccgatgac caggccctgc ggatcggttt ccggattcag ttcgatgatc
     5761 ctggtcacct tcaccccggt caggtgcgag atccgggtca gcgtgtcgcc ttcctccact
     5821 tcgtaggtcg ccgccgtgat tttcggtttt tcttcttttt cggttttggc gtgctgattc
     5881 gactgtttgc cgttcttcga ggagtcctcg tcgagcgcgc cggagacggc cacgtagacg
     5941 gccgccagcg ccgcgatgat cgcgaggagg gcaaagaggc gggcgaagac gctcgtgcgt
     6001 tttttcatca gaccgggtgg ggttcgaagc ggagccgctg atcgtagtat cggcagccgc
     6061 tctcaggcgg tcgaaatctt ccaaaccgtc tcccgcagct cctccgccgc ggcggccggg
     6121 tcctcggccc cggcgatgcc gcgcgaggcg gcgacgaggg cggccgagcg gccggcgctg
     6181 aaggcggggc cgagcagctc cggctggcca ccctgggcgc cgacgccggg gatcaggaag
     6241 gtcgcgcgcg gcatcagctc gcgcaggcgg ccgatgtgct ggggctcggt ggcgccgacc
     6301 accgcgccca tcccgctgag gccggactcg ccggcaaggc gcggagcgag accgtcgacg
     6361 atctcggcga gccgctcgtg cagggg
//
LOCUS       DASRIG010000014         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_528888, whole genome shotgun sequence.
ACCESSION   DASRIG010000014 DASRIG010000000
VERSION     DASRIG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528888"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFB52_00270"
     CDS             complement(<1..1220)
                     /locus_tag="VFB52_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZO04796.1"
                     /translation="MLDSGQRAIVGARVVAQNAATGQTREAQTDAAGRFTLPSLPIGT
                     YKLTVEQSGFQTQARAAVQVGVAETVTADFALAPGVVTETVEVKGGEAVLDTEGTASG
                     GKLNNRTLVELPINGRDYARFSLLQPGAVARSNFIADLSFNGLHTVHNQFAIDGIDAS
                     RVDQPYMSNGFERGARLLTGSLDTIAEFRVQGSNYQAQYGRAAGTFINIASKSGSNEF
                     HGTVFEFFRNDALDARNFFNTEPNPQAPFRFNDFGGNLGGPIIKNRTFFFVNYEGSRQ
                     RVGITGTGTVPSQLMREQTLAASPELRPILETMPLGTSTTTNPLVDNYSTAKTSQIRE
                     DTASVKFDHRFTENNAFYARVNVNDTRVFGPLFGVTPAALGLLDFQNVPVRTTNFAIN
                     DQHIFSPRLINEFT"
     gene            complement(1458..2921)
                     /locus_tag="VFB52_00275"
     CDS             complement(1458..2921)
                     /locus_tag="VFB52_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZO04797.1"
                     /translation="MTGILLAAALLLTTPPAATTQTGPAAPPNIIFIMTDDHAAHAIS
                     AYGSRLIRTPNIDRLAREGMRFDNCFVTNSICTPSRAAILTGKYAHLNGVPVFNHIDN
                     SQPMLQKYLQRAGYHTGMVGKWHLGGNNPARPEDGKPTGFDYWNILPGQGAYFDPEFI
                     DNGKRVKLQGYTTDIITDLALGFLKDRPQGKPFFLMYHHKAPHRNWQPDEKHRRQFEN
                     VIVPEPETFDDDYRGKSDAARAATMRIDRDLNENDLKVPPPAGLSGPELKKWKYQRYM
                     RDYLACVAAVDDNIGRLLAWLDENDLAKNTIVIYTSDQGFFLGEHNFFDKRFMYEESL
                     RMPFLVRWPGRVKPGAVSRGMILNVDFAPTLLAAAGVGVPADMQGRSFVPLLEGRAPK
                     DWRTSMYYRYYHPGHHNVAAHYGVRDERYKLIYFNKLDQWELYDLRKDPREMRNVYDD
                     PAHQGVAKRLKAELLRLRRELRDEDQFQDRLPPDDVG"
     gene            complement(2975..>3638)
                     /locus_tag="VFB52_00280"
     CDS             complement(2975..>3638)
                     /locus_tag="VFB52_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO04798.1"
                     /translation="SHFSFNVPGGRCETCQGDGTVTVEMQFLADVELQCEECKGARFK
                     AAILEVRYRGKNIHEVLEMTVREAMAFFSTVPRIANKLKVLDDVGLGYLRLGQSATTL
                     SGGEAQRVKLASYLSKKTGDHTLYIFDEPTTGLHFDDINKLLASFRRLLEAGGSLLVI
                     EHNLDVIKTADWVIDLGPEGGDGGGEVVAVGTPEEIMRVKASHTGRFLREHLDHAEAA
                     RG"
BASE COUNT          541 a         1165 c         1252 g          680 t
ORIGIN      
        1 aaggtgaact cgttgatcag ccgcggactg aagatgtgct ggtcgttgat cgcgaagttc
       61 gtcgtccgca ccggcacgtt ctggaaatcg agcaggccga gcgccgccgg ggtgaccccg
      121 aagagcggcc cgaagacgcg cgtgtcgttg acgttgaccc gcgcgtagaa ggcattgttc
      181 tcggtgaagc ggtggtcgaa cttgacgctc gccgtgtcct cgcggatctg cgaggtcttg
      241 gccgtgctgt agttgtcgac cagcgggttg gtggtcgtcg acgtcccgag cggcatggtc
      301 tcgaggatcg ggcgcagctc gggcgaggcc gcgagcgtct gctcgcgcat gagctggctc
      361 ggcaccgtcc cggtcccggt gatcccgacc cgctgccgcg agccctcgta gttgacgaag
      421 aagaaggtcc ggttcttgat gatcgggccg ccgaggttcc cgccgaagtc gttgaagcgg
      481 aaaggggcct gcgggttggg ctcggtgttg aagaagttcc gcgcgtcgag ggcgtcgttg
      541 cggaagaact cgaagaccgt cccgtggaac tcgttggacc cgctcttgct cgcgatgttg
      601 atgaaggtcc cggcggcgcg gccgtactgc gcctggtagt tgctcccctg cacccggaac
      661 tcggcgatcg tgtcgaggct cccggtgagc agccgcgcgc cgcgctcgaa gccgttcgac
      721 atgtacggct ggtcgacgcg cgaggcgtcg atcccgtcga tggcgaactg gttgtggacg
      781 gtgtgcaggc cgttgaacga gaggtcggcg atgaagttac tccgggccac cgccccgggc
      841 tggagcaggc tgaagcgcgc gtagtcgcgg ccgttgatcg gaagctcgac gagcgtccgg
      901 ttgttgagct tcccgcccga ggccgtcccc tcggtatcga gcacggcctc gccgcccttg
      961 acctcgaccg tctcggtgac gacccccggc gcgagcgcga agtcggccgt caccgtctcg
     1021 gccaccccga cctggaccgc ggcccgcgcc tgcgtctgga agcccgactg ctcgaccgtc
     1081 agcttgtagg tcccgatcgg gagcgacggc agcgtgaagc gcccggcggc gtcggtctgc
     1141 gcctcccgtg tctggcccgt cgccgcgttc tgcgcgacga cccgcgcccc gacgatggcg
     1201 cgctgccccg agtcgagcac ctgcccggtg atcgccccgg ccgtctgcgc gcccgccagc
     1261 gtcgccgcga gcagggttaa tgataagcct gtgagaagtg tggaaagttt ggaaagactt
     1321 ctaccgaaag agatcacggt ccgcctcctg tgtttgctgt ctagttgtgt ccggtgctgg
     1381 gatggaagcg tcttgccctg atctacggtc aggtggaagg attgtaacag aatagtgggg
     1441 cgcagggaac tgattcgtta cccgacgtcg tcgggcggca ggcggtcctg gaactggtct
     1501 tcgtcccgca gctcccgcct cagcctgagg agctcggcct tgagccgctt ggccacgccc
     1561 tggtgagcgg gatcgtcgta aacgttgcgc atctcgcgcg ggtctttccg caggtcgtag
     1621 agctcccact ggtcgagctt gttgaaatag atcagcttgt agcgctcgtc gcggacgccg
     1681 tagtgcgccg cgacgttgtg gtgccccggg tggtagtagc ggtagtacat cgaagtccgc
     1741 caatccttcg gggcccggcc ctcgagcagc gggacgaagc tgcgcccctg catgtccgca
     1801 ggcacgccga cgcccgccgc ggcgaggagg gtcggcgcga agtcgacgtt gaggatcatg
     1861 ccccggctaa cggcgccggg cttgacccgc cccggccagc ggacgaggaa cggcatgcgc
     1921 agcgactcct cgtacatgaa ccgtttgtcg aagaagttat gctcgccgag gaagaagccc
     1981 tggtccgacg tgtaaatgac gatcgtgttt ttcgccaggt cgttctcgtc gagccacgcc
     2041 agcagccgcc caatgttgtc gtccaccgcc gcgacgcacg ccaggtagtc gcgcatgtac
     2101 cgctggtact tccacttctt cagctccggc ccgctgaggc ctgccggggg cggtaccttg
     2161 aggtcattct cgttgaggtc ccggtcgatg cgcatggtgg ccgcccgcgc cgcgtccgac
     2221 ttgccccggt aatcgtcgtc gaaagtctcc ggctcgggca cgatgacgtt ctcgaactgc
     2281 cggcggtgct tctcgtccgg ctgccaattc cggtggggcg ccttgtggtg gtacatcagg
     2341 aagaaaggct tgccctgggg gcggtccttc aagaacccga gcgccaggtc ggtgatgatg
     2401 tccgtggtgt agccttgcag cttgacccgc ttcccgttgt cgatgaactc cgggtcgaag
     2461 tatgccccct gcccggggag gatgttccag tagtcgaagc cggtcggctt gccgtcctcg
     2521 ggccgcgccg ggttgttacc gccgaggtgc cacttgccaa ccatgccggt gtggtacccg
     2581 gcccgctgga ggtacttctg gagcatcggc tgcgagttgt cgatatggtt gaagaccggg
     2641 acgccgttga ggtgcgcgta cttgccggtg aggatcgccg cccggctcgg cgtgcagatc
     2701 gagttagtga cgaagcagtt gtcgaaccgc atcccctcgc gcgccaggcg atcgatgttt
     2761 ggcgtccgga tcagccgact gccgtaggcg ctgattgcgt gcgccgcgtg atcgtccgtc
     2821 atgatgaaga tgatgtttgg cggcgcggcc ggcccggtct gggtggtagc ggccggaggg
     2881 gtcgtcagca gcagcgctgc cgccagcaga ataccggtca caagcctcat aatctctccc
     2941 acctcaactg aagtctgatg cgcgccggcc cgcgtcaccc gcgggccgcc tcggcgtggt
     3001 ccaggtgctc gcgcaggaac cgcccggtgt gcgaggcctt gacccgcatg atttcttcgg
     3061 gcgtcccgac ggcgacaacc tcgccgcccc cgtcgccacc ctccggcccg aggtcgatga
     3121 cccagtcggc cgtcttgatg acatcgaggt tgtgctcgat gacgaggagc gacccgcccg
     3181 cctcgagcag gcgccggaac gaggcgagca gcttgttgat gtcgtcgaag tgcagccccg
     3241 tggtcggctc gtcgaagatg tagagcgtgt ggtcgccggt cttcttcgag aggtaggagg
     3301 cgagcttgac gcgctgcgcc tcgccgcccg agagcgtcgt cgccgactgg ccgagccgca
     3361 ggtagccgag gccgacgtcg tcgagcacct tgagcttgtt ggcgatccgc gggaccgtcg
     3421 agaagaaggc catcgcctcc cggaccgtca tctcgagcac ctcgtggatg ttcttgcccc
     3481 ggtaccggac ctcgaggatc gccgccttga agcgcgcccc cttgcactcc tcgcactgga
     3541 gctcgacgtc ggccaggaac tgcatctcga cggttaccgt cccgtccccc tggcaggtct
     3601 cgcagcgccc gcccgggacg ttgaacgaga agtggctc
//
LOCUS       DASRIG010000015         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1573801, whole genome shotgun sequence.
ACCESSION   DASRIG010000015 DASRIG010000000
VERSION     DASRIG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573801"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFB52_00285"
     CDS             complement(<1..648)
                     /locus_tag="VFB52_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04799.1"
                     /translation="MPALVSWVADPARAGQAAVEVSGLSPEVLRRLQESNWGLPQWQR
                     LLAVYAQPGQATIDPRLPPMLGAYGIRSAVLRFEPQFPLEPGVTYLAVFRPDHLPGGS
                     DAGGAAITASHRLPPREATPTTAVSHVYPTAEVLPENLLKFYIHFSAPMSRGQIYDHI
                     RLQDGAGKGIELPFLEIGEELWDPEMKRLTLFIDPGRIKRGVRPLEEVGPALEAGK"
     gene            complement(744..1919)
                     /locus_tag="VFB52_00290"
     CDS             complement(744..1919)
                     /locus_tag="VFB52_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04800.1"
                     /translation="MLPALLPSDSRSPRKEQLMKRTAAYVVLTLLALGLAARSAVAAG
                     WTADMKEGKPVFQSMGPLAFGPDGILFVADTKAAAIVAIATGDLSPAAGAKPIKVEGV
                     NQKIAALLGTSADQILIEDLAVNPVSRHVYLAVSRGRGPEAVPVLLRVKADGQLEVFS
                     LEKVKYSRAELPDAPADAVVGQGNRQSNPRRESITDLAFLDGRVLVAGLSNEEFASTL
                     RAIPFPFQAVGGGTGVEIYHGAHGRFETRSPVRTFVPFKVGNEAHLLAAYTCTPLVQF
                     PVTELKPGAKIKGKTIAELGNRNRPLDMIVYEKGGRTYLLMANSSRGVMKIGTDQIAG
                     AESIVAPVPDGAKKGLPYETIESWSGVDQLDRLDGQHVVVLRRAEGGSLNLESLPLP"
     gene            complement(2032..>2569)
                     /locus_tag="VFB52_00295"
     CDS             complement(2032..>2569)
                     /locus_tag="VFB52_00295"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZO04801.1"
                     /translation="AERHPFDTGRLRRVIELAAERSGWAKKKPGRGRALGLAAHRSFL
                     TYVAAVVEVEVDARGRVRIPRVDFAVDAGRVINPDRVKAQFEGAAVFGASIALMGEIS
                     AAAGQIQQSNFHDYPVARITEAPYETHVHLVPSQEAPAGVGEPGVPPIAPAICNAIFA
                     ATGKRIRDLPLSKHKLNA"
BASE COUNT          399 a          892 c          875 g          403 t
ORIGIN      
        1 cttgccagcc tcgagcgccg gccccacctc ttccagcggt cggaccccgc gcttgatccg
       61 gccgggatcg atgaacagcg tcagccgctt catctccggg tcccacagct cttcgccgat
      121 ctccaggaac ggcagctcga tccccttccc cgccccgtcc tgcaaacgaa tgtgatcgta
      181 gatctggcca cgactcatgg gcgcggaaaa atggatgtag aacttgagca gattctccgg
      241 gaggacttcg gccgtcgggt aaacgtggct gaccgcggtg gtcggagtcg cctcccgcgg
      301 cggcaggcgg tggctggccg tgatcgcggc gccccccgcg tcggagccac ccggcaggtg
      361 gtcgggccgg aagaccgcca ggtaggtgac ccccggctcg agcgggaact gcggttcgaa
      421 gcgcagcacc gccgaacgga tgccataggc gcccagcatc gggggcaggc gcggatcaat
      481 cgtcgcctgc cccggctgcg cataaacggc gagcaggcgc tgccactgcg gcagccccca
      541 gttcgactcc tgcagccgcc gcaagacttc cgggctcaag ccggagacct cgacggcggc
      601 ctgaccggcg cgcgccgggt cggcgaccca gctgacgagg gcgggcacgc cgcgagccgc
      661 cgccgcccca attagcactg atgacagcac taacgacagc gccgccgagg gcgcgcccca
      721 ccgtcgagct cgcgagatca tggtcagggc aggggcagcg attcgagatt gagcgagccg
      781 ccctcggcgc ggcgcagcac gacgacgtgc tggccgtcca ggcggtcgag ctggtccacc
      841 ccactccagc tttcgatggt ctcgtagggc agtcctttct tcgcaccgtc cggcaccggc
      901 gcgacgatgc tctcagcccc ggcgatctga tcggtgccga tcttcatgac gccgcgactg
      961 ctgttggcca tgaggaggta ggttcggccg cccttctcgt aaacgatcat gtcgagcggc
     1021 cggttgcggt tcccgagctc ggcaatggtc ttgcccttga tcttggcgcc gggcttaagc
     1081 tccgtgacgg ggaactgcac cagcggcgtg caggtgtagg cggccagcag gtgcgcctcg
     1141 ttgccgacct tgaagggcac gaaggtccgc accggcgagc gagtctcgaa gcgaccgtgg
     1201 gcgccgtggt agatctccac gcccgtccca ccgccgaccg cctggaacgg gaacgggatg
     1261 gcgcggaggg tcgaagcgaa ctcctcattc gagaggccgg caaccagcac gcggccgtcg
     1321 aggaaggcga ggtcggtgat cgactcgcgc cgcgggttgc tctggcggtt gccctggccg
     1381 acgaccgcgt ccgccggcgc gtcgggcaac tccgcccgcg aatatttgac tttctcgagc
     1441 gagaatacct ccagctggcc gtccgctttg acgcgcagga gcaccggcac cgcctccggg
     1501 ccgcgcccgc gcgagacggc caggtagacg tggcgcgaga ccgggttgac cgccaggtct
     1561 tcaatcagaa tctgatcggc gctcgtcccc agcagggcgg caatcttctg attgaccccc
     1621 tccactttga tcggcttggc gcccgccgcg gggctcaggt cgccggtcgc gatggcgacg
     1681 atcgccgccg ccttggtgtc ggcgacgaag agaatcccgt cgggcccgaa cgccagcggg
     1741 cccatggatt ggaagaccgg cttgccctcc ttcatgtcgg ccgtccatcc ggccgcgaca
     1801 gcgctccggg cggcgaggcc gagcgccagg agagtcaaga cgacgtaagc agcagtgcgt
     1861 ttcatcagtt gctcctttcg aggtgatcgg gaatctgacg gcaataacgc gggcagcatc
     1921 gggccggtta ctggaaaaat ctcatgacgg acggcgcgag ggagggcggc gacgggtcgc
     1981 cgccctcgac aggcctttgc ccgtgtcgcg ccgtgtcgtc agtcctcgac gctatgcatt
     2041 cagcttatgt ttgctgagcg gcaagtcccg aatgcgcttg ccggtcgcgg cgaagatggc
     2101 gttacagatc gccggcgcga tcggcggcac gcccggctcg ccgaccccgg ccggcgcctc
     2161 ctgactgggc accaggtgga cgtgcgtctc gtaaggcgcc tcggtgatcc gcgcgaccgg
     2221 gtaatcgtgg aagttggact gctggatctg gccggccgcc gctgagatct cgcccatgag
     2281 ggcgatgctc gcgccgaaga ccgccgcgcc ttcgaactgc gccttgacgc gatcggggtt
     2341 gatcacccgg cccgcgtcga ctgcgaagtc gacgcgcggg atcctcaccc tcccgcgcgc
     2401 gtccacctcc acctcgacta ccgccgcgac ataggtcagg aagctgcggt gcgccgcgag
     2461 gccgagggcg cgcccccggc cgggtttttt cttcgcccag cccgagcgct ccgccgccaa
     2521 ctcgatgacc cgccgcaggc ggcccgtgtc gaaggggtgt ctttcggcc
//
LOCUS       DASRIG010000016         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3398205, whole genome shotgun sequence.
ACCESSION   DASRIG010000016 DASRIG010000000
VERSION     DASRIG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398205"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VFB52_00300"
     CDS             complement(<1..1722)
                     /locus_tag="VFB52_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZO04802.1"
                     /translation="MGAVGRSRGAALAVALACALAIGGAERTRGASVPAPRPNVVVVM
                     SDDQTQDSMRYMGRVNELIGGRGATFPVSVTNWPLCCPSRATLLTGQYAHNHGVLGNT
                     PPFGGFDRLGASETLPVWLQRAGYYTAHIGKFLNGYENSAVGVPAGWSEWHGSKRTYL
                     FYGYELLEDGQRVAYGSTTEDPDAPTAPETYSTDAYTDKAVELISRRAPDRRPFFLSL
                     AYLAPHSGGPDPDAPNQSRCNNTAKPAARHIGAFANEPLPQPPNFNEADVSDKPAGFA
                     TRPALTPEQIANATRRYRCRAESLLAIDEGVQRVIGALRDSGELDNTLLIYTSDNGFF
                     HGEHRVALGKNRVYEEAIRVPLLMRGPGIPKDVSVDELAINADLAPTILDATGAEPGA
                     AVDGRSLLPVAERPRRLRGRELLIEQMAGDDDEDANGVFYSALRNSNYTYVSNASGET
                     ELYDLDADPFQLDNEVANPAYDAAEAALAARLARLRACAALTCRSKPALKLNLPGAER
                     RDGRSCRRAGRFVARVRGAAAGRLVFARFEVGGRASGSDQAAPFRKRLTPRLLRRKPR
                     PEIQVFAE"
     gene            complement(1853..3667)
                     /locus_tag="VFB52_00305"
     CDS             complement(1853..3667)
                     /locus_tag="VFB52_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZO04803.1"
                     /translation="MESATVDTAATEAATGSSTLADLFPSAARKHGSKRAVLYKHESG
                     HWVSKTYTEVGEIVRKLSLGLIDLGIEKGDKVAILSNTRPEWTYFDFAALSAGATVVP
                     IYQTNSPEECQYVLENSDAVAVVVEDDEQLEKIRQVLDRCPKLEHVIRMSGTSRDAIS
                     FEDLSERGAAGSESDWEQRYRSVGPDDICTFIYTSGTTGPPKGCVISHGNYRSMLDMV
                     HEVNVLGDDEVTYLFLPLAHSFALLIQFGTFDLGATLAYWERDPLKILPNLAEVKPTY
                     FPSVPRIFEKIYTAATANVEKEGGLKKTVFDWAIGVGKKVRRLEREGKRPGALLALQY
                     RIADKQVLSKIRGLFGGRLKLAVTGAAPINPEILEFFDAAGVLVLEGWGMTETSTAST
                     IATPEDFKWGTVGKPFPGCELKIAEDGEILVKGANIFQGYYKNEEATAETLVDGWLHT
                     GDIGEIDPDGYLKITGRKKDIIITAGGKNITPANLEAEIKQHPLVSQCVVIGDRRPFL
                     VALVTLDPEEAAKYASEHGLPPDPAALAENAEVKASIDKHVEKINEKFARVEQVKKIE
                     ILPQDLSQEGGELTPTMKVKRNVVADKYASEIEALYAG"
     gene            complement(3772..>3971)
                     /locus_tag="VFB52_00310"
     CDS             complement(3772..>3971)
                     /locus_tag="VFB52_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04804.1"
                     /translation="EEEKYVAVHLTPTELADEMNMKRRDVISKCMEMGVPIFNGRIDK
                     TLFLSNLRLFGEGQKTPQTAA"
BASE COUNT          553 a         1342 c         1392 g          684 t
ORIGIN      
        1 ctcggcgaag acctgaatct ccggtctggg cttgcggcgc agcaggcgcg gcgtgagccg
       61 cttccgaaac ggcgcggctt ggtcggagcc cgacgcccgg ccgccgacct cgaaccgcgc
      121 gaacacgagc cggccggcgg ctgcgccccg cacccgcgcg acgaaccggc ccgcccgcct
      181 gcatgagcgg ccgtctcggc gctccgcgcc cggtaggttc agcttcagag ccggcttcga
      241 gcgacaggtc agcgcggcgc aggcccgcag ccgcgccagt cgcgccgcca gcgccgcctc
      301 ggccgcgtcg taggcggggt tcgccacctc gttgtccagc tggaacgggt ccgcgtcgag
      361 gtcgtagagc tcggtctcgc cgcttgcgtt gctgacgtac gtgtagttgg agttgcggag
      421 cgcgctgtag aagacgccgt tggcgtcctc gtcgtcgtcg ccggccatct gctcgatcag
      481 gagctcgcgg ccccgcagcc ggcgcgggcg ttccgcgacc ggcaggagcg agcgtccgtc
      541 gaccgcggcg ccgggctcgg caccggtcgc gtcgagaatc gtcggcgcga ggtcggcgtt
      601 gatcgcgagc tcgtccacgc tgacgtcctt cgggatcccc ggcccgcgca tcaggagggg
      661 gacccggatc gcctcctcat agacgcggtt cttgccgagc gcgactcgat gctcgccgtg
      721 gaagaagccg ttgtcggagg tgtagatcag cagggtgttg tccagctcgc cggagtcgcg
      781 cagggcgccg atgacccgct ggacgccctc gtcgatcgcc agcagcgact cggcccggca
      841 gcggtagcgc cgagtggcgt tggcgatctg ctccggggtc agcgccgggc gggtagcgaa
      901 tcccgccggc ttgtcgctga cgtcggcctc gttgaaattc ggcggctggg ggagcggctc
      961 gttcgcgaac gccccgatgt gtcgcgccgc cggcttcgcc gtgttgttgc agcggctctg
     1021 gttcggcgcg tcggggtcgg gcccgccgct gtggggcgcg agatacgcga gcgacaagaa
     1081 aaacggccgg cggtcgggcg cccggcggga gatcagctcg accgccttgt cggtgtaggc
     1141 gtcggtcgag tacgtctccg gcgcggtcgg ggcgtcggga tcctcggtgg tggagccgta
     1201 ggcgacccgc tgaccgtctt cgagcagctc gtagccgtag aagaggtagg tccgcttcga
     1261 gccgtgccac tcggaccaac ccgcgggaac gcccacggcg ctgttctcgt agccgttgag
     1321 gaacttcccg atgtgcgccg tgtagtaccc ggcgcgctgc agccacaccg gcagcgtctc
     1381 ggacgcgccg aggcggtcga agcccccgaa cggcggcgtg ttgccgagca cgccgtggtt
     1441 gtgggcgtac tggcccgtga gcagcgtggc gcgcgacgga cagcagagcg gccagttcgt
     1501 cacgctcacg gggaaggtgg ccccgcggcc gccgatcagc tcgttgaccc gccccatata
     1561 gcgcatcgag tcctgggtct ggtcgtcgct catgaccacg acgacgttcg gccgcggggc
     1621 agggaccgag gcgccccggg tgcgctccgc gccgccgatg gccaacgcgc aggcgagcgc
     1681 caccgccagc gcagctcccc gggacctgcc aaccgcaccc attacccccg ctaacccgac
     1741 gggcgcggca aagtagctcg ccgccggggc ccggcgttcg ggcgcggccg gggcgcttgg
     1801 gccgacccct tattgccctt taccggggct cgagagctcc ggcggccgcg gctcagccgg
     1861 cgtacagcgc ttcgatctcg ctcgcgtact tgtcggcgac gacgtttcgc ttgaccttca
     1921 tcgtcggcgt gagctcgccg ccctcctggg agaggtcctg gggcaggatc tcgatcttct
     1981 tgacctgctc gacacgggcg aacttctcgt tgatcttctc cacgtgcttg tcgatcgagg
     2041 ccttcacctc ggcgttctcg gcgagcgcgg cggggtcggg aggcagcccg tgctcggagg
     2101 catacttggc cgcctcctcg gggtccaggg tgacgagcgc aaccaggaac gggcggcggt
     2161 cgccgatcac cacgcactga gagaccagcg ggtgctgctt gatctctgcc tccaggttcg
     2221 ccggggtgat gttcttgcct ccggccgtga tgatgatgtc cttcttgcgg cccgtgatct
     2281 tcagatagcc gtcggggtcg atctcgccga tgtcgccggt atgcagccag ccgtcgacca
     2341 gggtctcggc ggtcgcctcc tcgttcttgt agtagccctg gaagatgttc gcgcccttga
     2401 ccaggatctc gccgtcctcg gcgatcttca gctcgcaacc ggggaacggc ttgccgacgg
     2461 ttccccactt gaagtcctcc ggggtggcga tcgtcgacgc ggtcgatgtc tcggtcatcc
     2521 cccagccctc gagcacgagc acgccggcgg cgtcgaagaa ctcgaggatc tccgggttga
     2581 tcggcgcggc gccggtgacc gcgagcttca gccggccgcc gaacaggccc cggatcttcg
     2641 acagcacctg cttgtcggcg atccggtatt gcagcgcgag gagagcgccc gggcgcttgc
     2701 cctcgcgctc gagccgtcgc accttcttgc cgaccccgat cgcccagtcg aagacggtct
     2761 tcttcaggcc gccctccttc tcgacgttgg cggtagccgc ggtgtagatc ttttcgaaga
     2821 tccgcggcac cgacgggaag taggtcggct tcacctcggc gaggttgggc aggatcttca
     2881 gcgggtcgcg ctcccagtag gcgagcgtgg cgccgaggtc gaaggtgccg aactggatca
     2941 gcagcgcgaa cgagtgcgcg agcggcagga acagataggt gacctcgtca tcacccagga
     3001 cgttgacctc gtggaccatg tcgagcatcg accggtagtt gccgtgcgag atcacgcagc
     3061 ccttcggcgg cccggtcgtg cccgaggtgt agatgaacgt gcagatgtcg tccggcccga
     3121 ccgagcggta gcgctgctcc cagtcggact ccgagcctgc ggcgccgcgc tcggagaggt
     3181 cctcgaacga gatcgcgtcg cgcgaggtcc cgctcatccg gatgacgtgc tcgagcttcg
     3241 ggcatcggtc gaggacctga cggatcttct cgagctgctc gtcgtcctcg acgacgactg
     3301 cgaccgcgtc ggagttctcg aggacgtact ggcattcctc gggcgagttg gtctggtaga
     3361 tcgggaccac cgtcgcgccg gccgaaagcg ccgcgaagtc gaagtaggtc cactcgggac
     3421 gggtgttgga gaggatcgcg accttgtcac ccttctcgat cccgaggtcg atcagcccaa
     3481 gggagagctt gcgcacgatc tcgccgacct cggtgtacgt cttcgagacc cagtgcccgg
     3541 attcgtgctt gtagagcacc gcccgcttcg atccgtgctt tcgagcggcg gacggaaaca
     3601 ggtccgcgag cgtcgacgat ccggtcgcgg cctcagtcgc cgccgtatcg accgtggccg
     3661 attccatgct gctgtcctct cctcctgcgt tgacgaagga tccggcctcg tggccgccca
     3721 gcggaaagct acctcagcga ggcgagtacg gggacgccgg cgaggcgacc gctaggccgc
     3781 cgtctggggc gtcttctggc cctcgccgaa gagacgcagg ttggagagga agagggtctt
     3841 gtcgatgcgg ccgttgaaga tcgggacacc catctccatg cacttggaga tgacgtcccg
     3901 ccgcttcatg ttcatctcgt cggcgagctc ggtcggcgtc aggtgaacgg ccacgtactt
     3961 ctcctcttcc g
//
LOCUS       DASRIG010000017         7570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_534198, whole genome shotgun sequence.
ACCESSION   DASRIG010000017 DASRIG010000000
VERSION     DASRIG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534198"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..670
                     /locus_tag="VFB52_00315"
     CDS             218..670
                     /locus_tag="VFB52_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04805.1"
                     /translation="MAALVGVAALVGYAYQRHYLQERYEHVSFSVAGLNKAFDRFSDV
                     SGARIATTSTRQYPLFGDDLSNRVQFIGEEQPHGGFEAPTSCRRWRELLNEGDYDYVI
                     ATWDRIEPGKPPYPPQAAWTAGPGVQVVEKEEPTVVFKLDGQLDPSAC"
     gene            717..2510
                     /gene="lepA"
                     /locus_tag="VFB52_00320"
     CDS             717..2510
                     /gene="lepA"
                     /locus_tag="VFB52_00320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZO04806.1"
                     /translation="MEHIRNFSIIAHIDHGKSTLADRILEVTGAVDPTKMQAQMLDSM
                     DLERERGITIKAQAVRVEYTAADGQVYHLHLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQTVANTYLAIDAGLELIPVINKVDLPGAEPERVAAEIADLTGGDPEEALH
                     ISAKTGVGVPEVLEAIVERIPGPQGEPSAPPRALIFDSEFDQYRGVIAYVRMVDGSIR
                     KHEKVLAMQNNTEAEIDDLGFFRPAMTAVQGMEAGDVGYLITGIKDVNKLSVGDTLTA
                     QANPATEALAGYREVRPMVFCGVFPIDTDRYEDLRDALEKLSLNDAALSWEPETSEAL
                     GFGFRVGFLGLLHMEIVRERLEREYGLELLSTTPNVRYVVHKTNGETVEVRSPTDLPD
                     PGMIETVEEPYIRATIIAPSEQVGAVMELCQARRGEHVDMHYLSPVRVQIRYDMPLGE
                     IVLDFFDLLKSSTRGYASLDYEPIGNRASDLVKVDILLAGEKVDALSIIVHRDFSYEQ
                     GKRLAERLKKTIPRQLFDVPVQAAIGSNILARETVKALRKDVTAKLYGGDVTRKQKLL
                     KKQKKGKKRMKSVGRVEVPQEAFLAVLQIDE"
     gene            2569..3009
                     /locus_tag="VFB52_00325"
     CDS             2569..3009
                     /locus_tag="VFB52_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZO04807.1"
                     /translation="MPIKTDAVGKEWPAVTYQVGREKIKEYAAALGLDNPVHFDVGAA
                     REAGFRDVVAPPMFAVVYSGPALGPVLFDPEVEMNFAAMVHGGQVFEWGEPVCSGDEI
                     STTPKCLSIEEKGGKGFYVFETVSVNQDGAEVVRGTWTNIVRGV"
     gene            3011..3403
                     /locus_tag="VFB52_00330"
     CDS             3011..3403
                     /locus_tag="VFB52_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO04808.1"
                     /translation="MADFNAGDAVPELRVTPDKYLPHRYAGASGDFNPIHIDDEFAKM
                     VGLPRNILHGLYSMGLVAKANAALAGGDPRALKRLSVQFRGMGMPEQEIVVSGTVKSV
                     DGDRVVIDTVAAQGETQIIKNAEAELEL"
     gene            3448..4476
                     /gene="hrcA"
                     /locus_tag="VFB52_00335"
     CDS             3448..4476
                     /gene="hrcA"
                     /locus_tag="VFB52_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZO04809.1"
                     /translation="MLSPRQELILRLVVDAYLASAKPVPSKEIAEAPEVEWGPSTVRA
                     ELAALEAAGYLTHPHTSAGRVPTDAGYRLYVDLLLASDAVAPEARVELELSRLRREVD
                     EAMRETTAALAQVTDLVAMATAPQQSVAATIHRVEVLQLTPSKVVVIAIASTGDVARR
                     VFEYERPLDPGLVAWASSYLNEQLSGLGVGARMIATKLADPHLAPVEAEFVATLAPAF
                     TELESAPASDLYLDGTSRLLSDAHLPDLPRADQLMTALERRADVLAMLRSSLAERSVF
                     LWIGAENPQPELRSVSVVGANYGLGHRNLGTVGVVGPLRMDYATAIASVRVAARELSR
                     YFETVYDG"
     gene            4490..5620
                     /gene="dnaJ"
                     /locus_tag="VFB52_00340"
     CDS             4490..5620
                     /gene="dnaJ"
                     /locus_tag="VFB52_00340"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZO04810.1"
                     /translation="MPRDYYEVLGVGRDAGEAEIKKAFRGLARELHPDVNAHDPEAEE
                     KFKEAAEAYEVLSDAERRRTYDAYGHEGLRSGGFDPRASNFGSIDDIFQAFFGAGGGD
                     VFGFGGGRRGPAGGGDIALAIEIELSEVATGVRRELRYDAVGACEHCHGNGAEPGTPI
                     RSCERCGGVGQLRQVSNTPFGQMARAVTCDTCHGAGKVAETPCEVCGGNGRTPGERVR
                     EVEIPAGIEAGQRLRVQGAGHAGEPGAPAGDLYVEVDVTADERFQRDGTDLITVVSIP
                     ATEAMLGTTVTVPTLEGEEEIELGAGTQPGHEELLRGAGLPPLGGRRRGNQRVVVEVI
                     VPTNLSEEQREVAERLDGTLEPSNLEPQHGEGLFSRVRRAFG"
     gene            5617..6432
                     /locus_tag="VFB52_00345"
     CDS             5617..6432
                     /locus_tag="VFB52_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZO04811.1"
                     /translation="MIRLAVRCSPELADLVLAELTVLAPNGVEEDRGPGYVEYAIYGG
                     EGELPDLGELDAAVGEDRIEVSSTEIPDDWADRWRDFHKPLLVGDRLWLRPSWEPARE
                     GTVDVVVDPGQAFGTGAHPTTRLCLEYLIELAEAGEAVGPLTDLGCGSGVLAIAASKL
                     GWSPVSGYDHEQPALEASAANASINAVPLDLHQTNLREALPPLAPTVVANMTSPILKA
                     VAAQLSDPPQTLVCSGILPGELDEVSETFGSTGLRESDRRQEGDWAALLLRRI"
     gene            6450..6944
                     /locus_tag="VFB52_00350"
     CDS             6450..6944
                     /locus_tag="VFB52_00350"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZO04812.1"
                     /translation="MPLASYYGADYKIALFLHVLAVVLAFGPTFGYGIAFSVLPQYPK
                     SAPGILAGIQKIDRYLVNPGMIVILLAGIYLMISSDDVWKGDEAFIIVGFLAIIALFG
                     LQHAYFQPRVRQALEMAERDLKVGDTLSEEFQTNSQQTGQVGGLAGLIVVVTIFFMVY
                     KPFM"
     gene            6941..>7570
                     /gene="murI"
                     /locus_tag="VFB52_00355"
     CDS             6941..>7570
                     /gene="murI"
                     /locus_tag="VFB52_00355"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZO04813.1"
                     /translation="MSGASPPVAMFDSGVGGLTVLHECLVSLPQEDFLYLGDTEWFPY
                     GTRDPERLRARIARIAELLLERGAKLLVIACNTATSVGEDVAREVAAASGVKVVPVVT
                     PQAEIAAAITASGRVGVLATPNTVESGAYRRALEAQGRALEVTEVEAPELAPFIQNGS
                     LFDEETMEMARTYCAPLKAADVDTLILGCTHYPLVSPMLQRILGRDVRLV"
BASE COUNT         1199 a         2711 c         2547 g         1113 t
ORIGIN      
        1 ctcggcctcc ggccccgagg gcatgccgcg cgggttcgag tccggcctcc gctacctcgc
       61 accggccctg gtcctcggcc tcgctctgct cccaaccgcc cccgtccttc gcgaccgcct
      121 cgcgaaaatc gggagccgta ctccttttgt cgcccacagg gcgacaaaag gcaggggggc
      181 cgttacggaa gtgcaacgga agtgggtgct gggggggttg gcggcgctcg tgggggtggc
      241 ggcgctcgtc gggtacgcgt accagcggca ctatctgcag gagcgctacg agcacgtgag
      301 cttttcggtg gccgggctga acaaggcgtt cgatcgcttc agcgacgtca gcggcgcccg
      361 gatcgcgacg accagcacgc gccagtaccc gctgttcggc gacgacctct ccaaccgcgt
      421 ccagttcatc ggcgaagaac agcctcacgg cggcttcgag gcgccgacca gttgccgtcg
      481 ctggcgcgag ctgctgaacg agggcgacta cgactacgtg atcgccacct gggaccgaat
      541 cgagcccggc aaacccccgt atccaccaca ggcggcgtgg accgccggcc cgggggtcca
      601 ggtggtcgag aaagaggagc cgaccgtcgt cttcaagctc gacgggcagc tcgatccgtc
      661 ggcctgctga gcacggctcc gccggctcgc tttgcccttc atactccgcc gtccccatgg
      721 agcacattcg taacttttcg atcatcgccc acatcgacca cggcaagtcg accctggccg
      781 accgcatcct cgaggtcacc ggcgccgtcg acccgacgaa aatgcaggcc cagatgctcg
      841 actcgatgga cctcgagcgc gagcgcggga tcacgatcaa ggcccaggcc gtgcgggtcg
      901 agtacaccgc cgccgacggg caggtctacc acctgcacct gatcgacacg ccgggtcacg
      961 tcgacttctc ctacgaggtc tcgcgcagcc tcgccgcctg cgagggcgcg ctgctggtcg
     1021 tcgacgccgc ccagggcgtc gaggcgcaga cggtcgccaa cacctacctg gcgatcgatg
     1081 ccgggctgga gctgatcccg gtgatcaaca aggtcgacct gcccggcgcc gaacccgagc
     1141 gggtcgcggc cgaaatcgcc gacctgaccg gcggcgaccc tgaggaagcg ctccacatct
     1201 cggccaagac cggggtcggg gtgccggagg tgctggaggc gatcgtcgag cgcatccccg
     1261 gcccgcaggg cgagccgtcg gccccgccga gggcgctgat cttcgactcc gagttcgacc
     1321 agtaccgcgg cgtgatcgcc tacgtgcgga tggtcgacgg cagcatccgc aagcacgaga
     1381 aagtgctggc gatgcagaac aacaccgagg ccgaaatcga cgacctcggc ttcttccgcc
     1441 ccgcgatgac cgccgtccag ggaatggagg cgggcgacgt cggctacctg atcaccggga
     1501 tcaaggacgt caacaagctc agcgtcggcg acaccctgac cgcgcaggcc aaccccgcga
     1561 ccgaggcgct ggcgggctac cgcgaggtcc gcccgatggt cttctgcggc gtcttcccga
     1621 tcgacaccga ccgctacgaa gacctccgcg acgcgctcga gaagctctcg ctgaacgacg
     1681 ccgcgctgtc ctgggagccg gagacctcgg aggcgctcgg gtttggcttc cgggtcggtt
     1741 tcctcggcct cctgcacatg gagatcgtcc gcgagcgcct ggaacgcgag tacgggctgg
     1801 agctgctctc gacgacgccg aacgtgcgct acgtcgtcca caagaccaac ggcgagacgg
     1861 tcgaggtccg cagccccacc gacctcccgg atccgggcat gatcgagacg gtcgaagagc
     1921 cctacatccg ggcgacgatc atcgccccct ccgagcaggt cggcgcggtg atggagctct
     1981 gccaggcccg ccgcggcgaa cacgtcgaca tgcactacct gagcccggtg agggtgcaga
     2041 tccggtacga catgccgctc ggcgagatcg tgctcgactt cttcgacctg ctcaagtcct
     2101 cgacccgtgg ctatgcctcg ctcgactacg agccgatcgg caaccgcgcc tccgacctgg
     2161 tcaaggtcga catcctgctc gccggcgaga aggtcgacgc gctctcgatc atcgtccacc
     2221 gcgacttctc ctacgagcag ggcaagcgcc tcgccgagcg gctcaaaaag acgatccccc
     2281 gccagctctt cgacgtgccc gtccaggcgg cgatcggctc caacatcctc gcccgcgaga
     2341 ccgtcaaggc actgcgcaag gacgtcacgg cgaagctcta cggcggtgac gtcacccgca
     2401 agcagaagct cctcaagaag cagaaaaaag gcaagaagcg gatgaagtcg gtgggccggg
     2461 tcgaagtgcc ccaggaggcc ttcctcgcag tgctccagat cgacgagtag ttacatcgat
     2521 ctgtgaaggg atagggtttg gccagctagc caaaccgagg agcgagggat gccgatcaag
     2581 accgacgcag tgggcaagga atggcccgcc gtcacctacc aggtggggcg ggagaagatc
     2641 aaggagtacg cggccgcgct gggcctcgac aaccccgtgc acttcgacgt cggcgccgcc
     2701 cgcgaggctg gcttccgcga cgtcgtcgcg ccgccgatgt tcgccgtcgt ctacagcggc
     2761 ccggccctcg gcccggtgct cttcgacccc gaggtcgaga tgaacttcgc ggcgatggtg
     2821 cacggcggcc aggtcttcga gtggggcgag ccggtctgct ccggcgacga gatctcgacc
     2881 acgccgaagt gtctctcgat cgaggagaaa ggcggcaaag gcttctacgt cttcgagacc
     2941 gtctccgtca accaggacgg cgccgaggtc gtgcgcggca cctggaccaa catcgtcagg
     3001 ggggtctgac atggctgact tcaacgcagg cgacgcggtc cccgagctgc gggtgacccc
     3061 ggacaagtac ctgccgcacc gctacgccgg tgcctccggg gacttcaacc cgatccacat
     3121 cgacgacgag ttcgcgaaaa tggtcgggct gccccgcaac atcctccacg gcctctactc
     3181 gatgggcctg gtcgcgaaag ccaacgccgc ccttgccggc ggcgacccgc gggcgctgaa
     3241 gaggctctcg gtccagttcc gcggcatggg catgcccgag caggagatcg tcgtctccgg
     3301 cacggtgaaa tcggtcgacg gcgaccgcgt ggtgatcgac acggtggccg cgcagggcga
     3361 gacccagatc atcaagaacg ccgaggctga gctggagctg tagtgcccgc cggggggcgg
     3421 cgctgagccg cttcctaaga tgtaggggtg cttagtcccc gtcaggaact gattctgcgg
     3481 ctggtcgtcg acgcgtacct cgcgtcggcg aagcccgtgc cctcgaaaga gatcgccgag
     3541 gcccccgagg tcgagtgggg cccctcgacc gtgcgcgcgg agctggccgc gctggaggcg
     3601 gcgggctatc tgactcaccc gcacacctct gccggccggg tcccgaccga cgccggctac
     3661 cggctctacg ttgacctgct gctcgcctcc gacgcggtcg cgccggaggc gcgggtcgag
     3721 ctggagctct cgcggctgcg ccgcgaggtc gacgaggcga tgcgcgagac caccgcggcg
     3781 ctcgcccagg tgaccgacct ggtggcgatg gcgacggcgc cgcagcagag cgtcgccgcg
     3841 acgatccacc gggttgaggt tctgcagctg accccgagca aggtcgtggt gatcgcgatc
     3901 gcctcgaccg gcgacgtcgc ccgccgcgtc ttcgagtacg aacggccgct cgaccccggc
     3961 ctcgtcgcct gggcctccag ctacctgaac gagcagctca gcgggctcgg cgtcggcgcc
     4021 cggatgatcg cgaccaagct cgccgatccc catctggccc cggtcgaggc ggaattcgtc
     4081 gccaccctcg cgcccgcctt caccgagctg gagagcgcgc ccgcttccga cctctacctc
     4141 gacggcacct cgcgcctgct ctccgacgcc cacctgcccg acctgccgcg cgccgaccag
     4201 ctgatgacgg cgctggagcg gcgcgccgac gtgctggcga tgctgcgctc ctcgctcgcc
     4261 gagcgctcgg tcttcctctg gatcggcgcc gagaacccgc agccggagct gcgctcggtc
     4321 agcgtcgtcg gcgccaacta cgggctcggc caccgcaatc tcggcaccgt cggcgtggtc
     4381 ggtccgctgc ggatggacta cgcgaccgcg atcgcctcgg tccgcgtcgc cgcgcgcgag
     4441 ctctcccgct acttcgagac cgtctacgac ggctagcctg agccgctaga tgccacgcga
     4501 ctactacgag gtgctgggcg tcggccgcga cgccggcgag gccgagatca aaaaagcctt
     4561 ccgcggcctc gctcgcgaac tgcacccgga cgtcaacgcc cacgaccccg aggcggagga
     4621 gaagttcaag gaggcggccg aggcctacga ggtgctctcc gacgccgagc gccgccgcac
     4681 ctacgacgcc tacggtcacg agggcctgcg ctccggcggc ttcgacccgc gagccagcaa
     4741 cttcggctcg atcgacgaca tcttccaggc cttcttcggg gccggcggtg gcgacgtctt
     4801 cggcttcggc ggcggccgcc gcggtccggc cggcgggggc gacatcgcgc tcgcgatcga
     4861 gatcgagttg tccgaggtcg ccaccggcgt gcgccgcgag ctccgctacg acgcggtcgg
     4921 cgcctgcgag cactgccacg gcaacggcgc cgagcccggc acgccgatcc gcagctgtga
     4981 gcgctgcggc ggcgtcggcc agctgcgcca ggtctcgaac accccgttcg gccagatggc
     5041 ccgtgcggtc acctgcgaca cctgccacgg cgccggcaaa gtcgccgaga cgccctgcga
     5101 ggtttgcggc ggcaacggcc gcacccccgg cgagcgcgtg cgcgaggttg agatcccggc
     5161 cgggatcgaa gcggggcagc ggctgcgcgt gcagggcgcg ggccacgccg gcgaacccgg
     5221 ggcgcccgcc ggcgacctct acgtcgaggt cgacgtcacg gccgacgagc gcttccagcg
     5281 cgacggaacg gacctgatca ccgtcgtctc gatccccgcc accgaggcga tgctcggcac
     5341 caccgtgacc gtgccgaccc tggaggggga ggaggagatc gagctcggcg ccggcaccca
     5401 gcccggccac gaagagctct tgcgcggggc cggcttgccg ccgctcggcg gccgccgtcg
     5461 cggcaaccag cgtgtcgtcg tcgaggtgat cgtcccgacc aacctcagcg aggagcagcg
     5521 cgaggtcgcc gagcggctcg acgggacgct cgagccctcg aacctcgagc cccagcacgg
     5581 cgagggcctg ttcagccgcg tccgccgcgc cttcgggtga tccggctcgc ggtccgctgc
     5641 tcgcccgagc tcgcggacct ggtgctcgcc gagctgaccg tgctcgctcc caacggggtc
     5701 gaggaggacc gcggccccgg ctacgtcgag tacgcgatct acggcggcga gggggagttg
     5761 cccgacctcg gcgagctcga tgcggcggtg ggggaggacc gcatcgaggt cagctcgacc
     5821 gagatccccg acgactgggc cgatcgctgg cgcgacttcc acaagccgct cctggtcggc
     5881 gaccgtctct ggctgcgccc ctcctgggag cccgcccgcg agggcacggt cgacgtcgtc
     5941 gtcgaccccg gccaggcctt cggcaccggc gcccacccaa ccacccgcct ctgcctcgag
     6001 tacctgatcg agctggccga agccggcgaa gcggtcgggc cgctgacgga cctgggctgc
     6061 ggttcgggcg tcctcgcaat agccgcctcg aagctcggct ggagcccagt cagcggttac
     6121 gaccacgagc agcccgccct agaggcgtca gccgccaacg cctccatcaa cgcagtcccg
     6181 ctagacctgc accagacgaa cctccgcgaa gcgctgcccc cgctagctcc cacggtcgta
     6241 gccaacatga cctccccgat cctcaaggcg gttgccgcac agctctcaga ccctccgcag
     6301 accctcgtct gctcagggat cctgccgggg gagctggatg aggtgtcgga gacgttcggg
     6361 agcaccggtc tgcgcgagtc agatcgccgc caagagggcg actgggccgc actcttgctt
     6421 cgtcggatct gagagatagg gttcccgcga tgccactcgc gtcctactac ggcgccgact
     6481 acaagatcgc gctgttcctg cacgtcctcg cggttgtgct cgctttcggc ccgaccttcg
     6541 gctacgggat cgccttctcg gtgctgccgc agtacccgaa gtccgcgccc gggatcctcg
     6601 ccggaatcca gaagatcgac cgctatctgg tcaacccggg catgatcgtg atcctgctcg
     6661 ccgggatcta tctgatgatc agcagcgacg acgtctggaa gggcgacgag gcgttcatca
     6721 tcgttggctt cctcgcgatc attgccctct tcggccttca gcacgcctac ttccagccgc
     6781 gcgtccgcca ggcgctggag atggccgaac gcgacctcaa agtcggggac acgctgagcg
     6841 aggagtttca gaccaacagc cagcagaccg gccaggtcgg cgggctcgcc ggtctgatcg
     6901 tcgtcgtgac gatcttcttc atggtttaca agccgttcat gtgagtggcg catcgccccc
     6961 ggtggcgatg ttcgactcgg gcgtcggtgg cctcaccgtg ctgcacgagt gcctggtctc
     7021 gctgccgcaa gaggacttcc tctacctcgg cgacaccgag tggttcccct atggcacgcg
     7081 cgaccccgag cgtttgcggg ctcgcatcgc ccggatcgcc gagctgctgc tggagcgcgg
     7141 cgccaagttg ctggtgatcg cttgcaacac cgccacctcg gtcggcgagg acgtcgcccg
     7201 cgaggtcgcg gcggcaagcg gggtgaaggt ggtgccggtg gtgacgccgc aggccgagat
     7261 cgccgccgcg atcaccgcca gcggccgggt cggcgtgctg gcgacgccga acacggtcga
     7321 gagcggtgcc taccggcggg cgctggaagc ccagggccgg gcgctcgagg tgaccgaggt
     7381 cgaggcgccg gagcttgctc cgttcatcca gaacggctct ctcttcgacg aggagacgat
     7441 ggagatggcg cgcacctact gcgcgccgct gaaggcggcg gacgtcgaca cgctgatcct
     7501 cggctgcacc cactacccgc tggtctcgcc gatgctgcag cggatcctcg gccgcgacgt
     7561 gcgcctcgtc
//
LOCUS       DASRIG010000018        14234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1833700, whole genome shotgun sequence.
ACCESSION   DASRIG010000018 DASRIG010000000
VERSION     DASRIG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14234
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833700"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFB52_00360"
     CDS             <1..1384
                     /locus_tag="VFB52_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZO04814.1"
                     /translation="TARGPMTLLDRPLRDQAATIAAGEADAGELLEATLARIEERNQA
                     LNAVVETFPERSREMLAAAPRGPLHGVPIVIKDEWPLPWRAQRFGAAEMLAPSAPGES
                     GPYRALRDAGAVVVGVGNMHELGSGSTGNVSFYGPASNPWNLERCPGGSSSGPASAVA
                     ARLVAGAVGADGVGSIRYPAAYCGLTGLKPTFGRSAMAGHHMADYSTMIVSGPLCADA
                     ADARLLGSVLCGEELAAGEARGLRIGVVRDLVSEDVAPDVRAACESAIATLAETSGGE
                     LVEIELPDLDASALAGVLIANTEGMNGISPEKLNNLNPELSPLGRGLLKYRMLLPAAA
                     TVKAAQVRALMRQRVAALFDRVDLIAWPSVPAVAPPLEAPLVELPSGVTTADQANVRG
                     GALANLTGIPAISLPVGLSEGLPIGLQLQAAWGRDALLLDAAEALERANGREWVEALP
                     PLAAAESAEA"
     gene            1386..2357
                     /locus_tag="VFB52_00365"
     CDS             1386..2357
                     /locus_tag="VFB52_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO04815.1"
                     /translation="MPAIVASGLERAFGEVLAVQGIDLSVEEGEIYGFLGPNGAGKTT
                     TVRMLTTLLLPTGGSASVAGHDVNSEARAVRGSIGVALQEAALDPLMTGRELIRLQAT
                     LQGIPTAEGRSRADSLLERVGLEGAADRRVGTYSGGMQRRLDLAAALVHEPRVLFLDE
                     PTTGLDPVSRKTIWEEVRALNNDGTTVFLTTQYLEEADQLADDVGIIDNGKIVAEGTP
                     ESLKAEIGNPHIQLQLAEGSVAEAEAKLSEVGRLLPPKDGRCILVEAANGAADIPRVV
                     RVLDEAGIAVESLELVKPTLDDVFVAKTGKHLASEDEEDAEQEPVGA"
     gene            2354..3145
                     /locus_tag="VFB52_00370"
     CDS             2354..3145
                     /locus_tag="VFB52_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04816.1"
                     /translation="MSRAGLATNLRVVRGLGMRSVRQTFRRPQLIAPIIVFPTLLLAI
                     QTGGAAASVELPGFPEVQSFLQFMLAGAMMQSMMLAGNSGGIALAVDIEMGFVDRLFA
                     APIPRFAIVLGRLAGTAALGLFAAVWFLAIGLIFGAEIEGGVPGALLAVALITASALA
                     VGGLGAAIALRTGSASVVQGLFPLVLVLLFLSSAFFPEELMIEPAKTIAEYNPLSFIV
                     EGVREPIISGIDATDTLIAVLAILGIVVVGLALSAGALRHRLRVG"
     gene            3145..3936
                     /locus_tag="VFB52_00375"
     CDS             3145..3936
                     /locus_tag="VFB52_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04817.1"
                     /translation="MEATATNSAWRVNLATIAALMARSKNELLRVPGAAIPGVLAPTI
                     FFLGLNGVFGALTQLNGFTTGSYEAFIVPVSMLQGAGFTGAAAGVNLARDIEAGWVDR
                     LLVSPAPRWVLLAGNVISASVRAFIPATFVLAIALALGAGWPGLDGLLIAYVMVGAMA
                     AVGACWGSFLALRAKSQSAAPLMQSGMMALTLLTPAYAPLALLSGWLHAVAEANPATE
                     VLEAARQGFVGTVTWADTWPGLLAVAGLLVVLGALALREMRRTAA"
     gene            3933..5135
                     /locus_tag="VFB52_00380"
     CDS             3933..5135
                     /locus_tag="VFB52_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04818.1"
                     /translation="MSETARAAVAAEAERVLRQNWHEGEHAGRRFAYTEPSPGRYPWQ
                     WYWDSCFAAIVWRRYEAGRSKAELESLLGAQEPNGFVGHTIFWRQPVTWPRLAFYNVA
                     TRSGFLQTRTIQPPLLAWAWKIAVGDPAAEPRIAAQADWLAAHRDLEGDGLLWIVQPD
                     ESGLDASPKFEEVWGRRANGRIGFPLLVRRNRRLGFDARRIRERGGPLLCEALVNTLW
                     SLSLQALGRPSATPALVERLWDERRGLFVDEAQPGTIRPHTVTWSSLAPLALPDLPEA
                     IGRRLVEEHLLNEREFLTPVAPPSVAANEPSYEPDGGHGPIRRYWRGPTWVNSAWLVW
                     LGMRRLGYEREAERLADGVIAAAAREGLREYYDPRTGKGLGAKDFAWSALVAELASPD
                     EGAAASYL"
     gene            complement(5126..5344)
                     /gene="infA"
                     /locus_tag="VFB52_00385"
     CDS             complement(5126..5344)
                     /gene="infA"
                     /locus_tag="VFB52_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZO04819.1"
                     /translation="MAKEEKIEMEGEITEALPNTMFRVKLDNGHDVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLDRGRITYRYK"
     gene            complement(5395..6840)
                     /locus_tag="VFB52_00390"
     CDS             complement(5395..6840)
                     /locus_tag="VFB52_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO04820.1"
                     /translation="MVDKIDMLAEAPVVIAVREALGDAEGVWIAGGAVRDAVLGIEVA
                     DLDLAVGGDPGAVAKAIGKGLGEHAFELSAEFGTWRVTSNQKGWQVDVTLLRGDSIEA
                     DLGERDFTIGAMAAPLADPAQLLDPHGGLADLQKRLLRVVSERSFEDDPLRLLRAARF
                     AAQLDLEVDAATVSLARKAAAHAADPAGERQLAELRQLLGGPNPVRGLSLLDELELTT
                     VVLPEVEGLRGVEQGPNHHLDVHGHTIAVLEHTLEVERDLPRFAGERADEVEALLEEP
                     LADEVSRRTALRFGALLHDIAKPATRSEQGGFVGFRGHDVVGAETIGTICARLRASRR
                     LTQHLQGLALHHLRLGFMIPEAPLPPRRIHDYLRATEPVGVDVTLLTIADRLSARGAG
                     PLARPEMVEAHLSLAREMIAAALDWRREGPPAPLLRGDELAAELGIEPGPELGKAMAE
                     LEAAQYAGELSDRAAAVEHARNWSTWSEEDA"
     gene            complement(6848..7963)
                     /gene="alr"
                     /locus_tag="VFB52_00395"
     CDS             complement(6848..7963)
                     /gene="alr"
                     /locus_tag="VFB52_00395"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZO04821.1"
                     /translation="MERAVARINFDAIRANCARLKAELGEGTELCAVVKADGYGHGAD
                     ACAGAAIAGGATWLAVATAVEAEGVGRRYRHIPLLTLGALTAAEVDIVLSASSEVSVW
                     REGFRAALAERASAQGAHARVHVKYDSGMGRLGNPDPDEVLALARACDADPNLELAGV
                     WTHFATADEPESDFFDEQLARFRDVAEAVKAEFPNVIAHAANSAAVYRDRAAHFDMAR
                     CGVAIYGLDPFQGDPAERGLVPALSLRSYVADVKRFPAGASAGYGQRWQAPEDTWVGV
                     IPLGYGDGVRRALSNNAEVLVGGRRYPLVGTVSMDNVTIDLGPETTVEPGAEAVLIGR
                     QGEEEIRTEEVAQRLDTINYEVTCGISSRVPRRPVSI"
     gene            complement(7974..9440)
                     /locus_tag="VFB52_00400"
     CDS             complement(7974..9440)
                     /locus_tag="VFB52_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZO04822.1"
                     /translation="MEPWLEPLYDARGIRAIDGWAIEEQGVPSAGLMATAGRALAEAV
                     AELTPQGPIRVLCGKGNNGGDGEIAAGRLADLGYEVETALLFEDGAPQDFDAWLAGSG
                     AVVDAIFGTGFAGAPREPAAAAIAAINRCGAPVVACDIASGVDASSGEIAGEAVEADL
                     TVSFHAAKIGQRVAPGKWHTGELRVVPIGIPEGAPIDPAAGTIGPAVLALAPARGARS
                     TKFSSGQVTIAGGSRGLTGAVRMSSSAAIRAGAGYATVAIPTDLEPVFELGQPEVMSV
                     GCPGGDGCLVPASAKAAIAAFERAAAGVLGPGMGRDPGTIELAHELAAGVTCPLVIDA
                     DGLNSFGGQLWRVGERTAPTVLTPHAGELGRLLGRPSEEIDAHRLAAAREAAAEAEAV
                     VVLKGDDTIVTDGTRVAVNVVSAPALATAGTGDVLSGIVAALLARGLEPFAAACAAVL
                     AHAHAGLDATARIGAAESVIATDVIESIPAGLRYAPVE"
     gene            complement(9475..11025)
                     /locus_tag="VFB52_00405"
     CDS             complement(9475..11025)
                     /locus_tag="VFB52_00405"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HZO04823.1"
                     /translation="MRVRLVLLLALATAAIALVVAGCGGGDGGGSDPASVMPAETPLY
                     IGVTVRPEGDLQANTEALAENVAGVDLSEEIVSALEQGAFTEEEDLDFEKDIEPWLGD
                     NAGMAFQELAGGDPEGVVIAVESTDAGAAEEFIEDFSTEVGDEPTYEGTTFKVEEDDG
                     TAVGMVDDYFVIAEDETIFKAVVDAAADESLADQDTYGSAVDATADESLADVFVDVGN
                     VLEETGTEIDAETQQLLGATGIEPKEAFVIASVVPGAESLEIDFATNAAGDNPPTGDA
                     SQLLGSLPGGSFAAFAAGEFGARLGESIDLIDEMGIEGQIEPNEFKDALKANGIDLDK
                     LTGGIGDLGVFAQGNTEANLTGAVVLTTKDAKEATNTVSNLGLLLRASNTPGVTALSG
                     DAAGFSIRDPELGDQPLVVAAKDDKIAISYGLAASTQALTAGKGSTLAENPTFEKAGD
                     ALGDTPLSGYIAGPATLALVDNLLSPEEAEDFESARPYVEKIEYLAIGNGTSGDLATS
                     KLIIGFSK"
     gene            complement(11060..12910)
                     /gene="glmS"
                     /locus_tag="VFB52_00410"
     CDS             complement(11060..12910)
                     /gene="glmS"
                     /locus_tag="VFB52_00410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZO04824.1"
                     /translation="MCGIVGYVGGRPCEELLLQGLEKLEYRGYDSAGLSVLEDGEVES
                     VHAVGNLANLRAAVDARADNGAGALPPSNTGIAHTRWATHGRVTEENAHPHDDSSGKV
                     HIVLNGIVENHASLRKRLQAEGEQFTSETDAEVVAHLIAHHYDGDLAAAVRAAYADLR
                     GHYAFVAMHADEPQRLLGARKECPLVAGVGEGETFLASAIPAFLADTRVAMPIENGEI
                     VEIEAGGVRISDAEGNPVERAPEEVTWDADAAEKGGYETFMLKEIHEQADAVAETIAD
                     RLPGDEGVDLSDVELSDEFLRGLNRIVIVACGTSYHAGLVGRYAIEEWARVPVEMDIA
                     SEYRYRNPVVGPGDLVVGMTQSGETADTLAAMRLAREAGATVLAVTNIMGSQVTREAD
                     AVLYTRAGLEVSVAATKTFVAQVAAMYLLGLRLAELRGTLPAERIAELVAEMKHLPSR
                     IEETLEMTEEPVKAVSAAHYSQEFFLYLGRHIGLPICLEGALKLKEISYVPTDAYAAG
                     EMKHGPIALLDESTPVVCVATDSPVLEKVLSNVEEVRARGAQTIAITTEGSERAGEVA
                     DETIAVARTDWILQPILAILPLQLLAYDIARARGLNVDQPRNLAKTVTVE"
     gene            complement(12942..>14234)
                     /gene="glmM"
                     /locus_tag="VFB52_00415"
     CDS             complement(12942..>14234)
                     /gene="glmM"
                     /locus_tag="VFB52_00415"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZO04825.1"
                     /translation="FGTDGIRGEAGTFLSAELATSLGRAVTASLEADRPQILIVRDTR
                     ESGPMLEAALAAGVAAAGGDALLGGVLPTPAAAILVKRLGLDLAAVVSASHNPFQDNG
                     IKFFNGQGTKLGDELEAQIEALMEEAPPATVGAGSVRELNGGLDDYLRELTAAFPLDL
                     SGRHIVLDCANGATFRAGPAIFERLGARVETIGVEPDGRNINAGCGSTHMETLASQVL
                     ASGADLGFAFDGDGDRVLAVDATGRVHDGDELIALGARGMAARGQLGGVVVTVMTNYG
                     FHAAMAEDGIEVAVTQVGDRYVIDEMLRRGWQLGGEQSGHIIATDFVATGDGIAAALM
                     TMRELGERGLDHAAPMEKLPQTLVNVEVADREAVNDAATVWEAVERENEALTGRGRVL
                     LRPSGTEPLVRVMAEAPSAEEAEAVCGRLVAIVREELG"
BASE COUNT         1898 a         5086 c         5180 g         2070 t
ORIGIN      
        1 aaccgcccgc ggcccgatga ccctgctcga ccgtccgctg cgcgaccagg cagccacgat
       61 cgcggccggc gaggccgacg ccggcgagct gctcgaggcg acgctggcgc ggatcgagga
      121 gcgcaaccag gcgctgaacg cggtggtcga gaccttcccg gagcgctccc gcgagatgct
      181 cgccgccgct ccgcgcgggc cgctgcacgg cgtcccgatc gtgatcaagg acgagtggcc
      241 gctgccctgg cgcgcgcagc gcttcggcgc cgccgagatg ctggcgccca gcgccccggg
      301 cgaatccggc ccctaccggg cgctgcgcga cgcgggcgcg gtagtcgtcg gggtcggcaa
      361 catgcacgag ctcggctcgg gcagcaccgg caacgtctcc ttctacggcc ccgccagcaa
      421 cccctggaac ctcgagcgct gcccgggagg ctcctccagc ggcccggcct ccgcggtcgc
      481 cgcccgcctg gtcgccggcg cggtcggcgc cgacggggtc ggctcgatcc gctacccggc
      541 cgcctactgc ggcctgaccg ggctgaagcc gaccttcggc cgcagcgcga tggccggcca
      601 ccacatggcc gactactcga cgatgatcgt ctccgggccg ctctgcgccg acgccgccga
      661 cgcgcgcctg ctcggctcgg tcctctgcgg tgaagagctg gccgccggag aggcccgcgg
      721 gctgcgaatc ggcgtcgtcc gcgacctcgt ctccgaagac gtcgccccgg acgtgcgcgc
      781 cgcctgcgag agcgcgatcg cgacgctggc cgagacgagc ggcggcgagc tggtcgagat
      841 cgagctgccc gacctcgacg cctcggcgct ggccggggtg ctgatcgcca acaccgaggg
      901 gatgaacggg atcagccccg agaagctcaa caacctcaac ccggagctga gcccgctcgg
      961 acgcggcctg ctcaagtacc ggatgctgct gccggcggcc gcgaccgtga aggccgcgca
     1021 ggtacgggcg ctgatgcgcc agcgggtcgc cgccctcttc gaccgcgtcg acttgatcgc
     1081 ctggccgagt gtgcccgcgg tggcgccgcc gctggaggcc ccgctggtcg agctgccctc
     1141 gggggtgacg accgccgacc aggccaacgt gcgcggcggc gcgctcgcca acctcaccgg
     1201 gatcccggcg atcagcctgc ccgtcggcct ctccgaagga ctgccgatcg ggctccaact
     1261 gcaggcggcc tgggggcgcg acgcgctgct gctcgacgcc gccgaggcgc tggagcgggc
     1321 gaacgggcgc gagtgggtcg aggcgctgcc gccgcttgcg gcagccgagt cggcggaggc
     1381 atagcgtgcc ggcaatcgtc gccagcgggc tggagcgcgc cttcggcgag gtgctggccg
     1441 tgcagggaat cgacctcagc gtcgaggagg gtgagatcta cggcttcctc ggacccaacg
     1501 gcgccggcaa gacgacgacg gtgcggatgc tgaccacgct gctcttgccg accggcggca
     1561 gcgccagcgt cgccggccac gacgtgaact cggaagcgcg cgcggtacgc ggctcgatcg
     1621 gcgtcgccct gcaggaagcg gcgctcgacc cgctgatgac cggccgcgag ctgatccgcc
     1681 tgcaggcgac cctccagggc atcccgaccg ccgagggccg cagccgcgcc gacagcctgc
     1741 tcgaacgggt cgggctcgag ggcgccgccg accgccgcgt cggcacctac tcgggcggga
     1801 tgcagcgccg cctcgacctc gccgcggcgc tcgtccacga accgcgcgtg ctcttcctcg
     1861 acgagccgac caccgggctc gacccggtca gccgcaaaac gatctgggag gaggtgcggg
     1921 cgctgaacaa cgacggcacc acggtattcc tcaccaccca gtacctcgag gaggccgacc
     1981 agctcgccga cgacgtcggg atcatcgaca acgggaagat cgtcgccgaa ggcacgccgg
     2041 agtcgctgaa ggcggaaatc ggcaaccccc acatccagct gcagctcgcc gagggctcgg
     2101 tcgccgaggc ggaggcgaag ctgagcgagg tcggccgtct gctgccgccc aaggacggcc
     2161 gctgcatcct ggtcgaggcc gcgaacggcg ccgccgacat cccgcgcgtg gtccgggtgc
     2221 tcgacgaggc cgggatcgcg gtcgagtcgc tggagctggt caaaccgacg ctcgacgacg
     2281 tcttcgtcgc caagaccggc aaacacctgg cgagcgagga cgaggaggac gccgagcagg
     2341 agccggtcgg ggcatgagcc gcgccgggct ggcgaccaac ctgcgggtcg tgcgcgggct
     2401 cgggatgcgc tcggtgcgcc agaccttccg ccggccgcag ctgatcgcgc cgatcatcgt
     2461 cttccccacc ctgctgctcg cgatccagac cggcggcgcg gcggcttcgg tcgaactgcc
     2521 cggcttcccg gaagtgcaga gcttcctcca gttcatgctc gccggggcga tgatgcagtc
     2581 gatgatgctg gccggcaaca gcggcggcat cgccctcgcg gtcgacatcg agatggggtt
     2641 cgtcgaccgc ctcttcgccg cgccgatccc gcgcttcgcg atcgtgctcg ggcggctcgc
     2701 ggggaccgcg gcgctgggcc tgttcgcggc ggtctggttc ctcgccatcg ggctgatctt
     2761 cggcgccgag atcgagggcg gcgtccccgg tgcgctgctc gccgtggcgc tgatcaccgc
     2821 ctcggcgctc gcggtcggcg ggctcggcgc ggcgatcgcg ctgcgcaccg gcagcgccag
     2881 cgtcgtccag ggcctcttcc cgctggtcct cgtcctgctc ttcctctcct ccgccttctt
     2941 ccccgaggaa ctgatgatcg agccggcgaa gacgatcgcc gaatacaacc cgctcagctt
     3001 catcgtcgaa ggggtgcggg agccgatcat ctccgggatc gacgcgaccg acacgctgat
     3061 cgcggtgctg gcgatcctcg gcatcgtcgt cgttgggctg gccctcagcg ccggcgccct
     3121 tcgccaccgc ttgcgggtcg gctgatggaa gcgaccgcga cgaattccgc ctggcgggtc
     3181 aacctggcga cgatcgccgc gctgatggcg cgctcgaaga acgagctgct gcgggtgccg
     3241 ggggcggcga tccccggggt gctggcaccg acgatcttct tcctcggcct gaacggcgtg
     3301 ttcggggcgc tgacccagct gaacgggttc acgaccggca gctacgaggc cttcatcgtc
     3361 ccggtcagca tgctccaggg ggccggcttc accggcgccg ccgccggggt caacctcgcc
     3421 cgcgacatcg aagccggctg ggtcgaccgg ctgctcgtct cccccgcccc gcgctgggtc
     3481 ctgctcgccg gcaacgtgat ctcggcgagc gtgcgggcat tcatcccggc gaccttcgtg
     3541 ctggcgatcg cgctggcgct gggcgccggc tggcccggtc tcgacggcct tctgatcgcc
     3601 tacgtgatgg tcggcgcgat ggccgcggtc ggcgcctgct ggggcagctt cctggcgctg
     3661 cgcgcgaaga gccagtcggc ggcgccgctg atgcagtcgg gaatgatggc gctgaccctg
     3721 ctgacccccg cctacgcgcc gctggccttg ctctccggct ggctgcacgc ggtcgccgag
     3781 gcgaacccgg cgaccgaggt gctggaagcg gcgcggcagg gcttcgtcgg caccgtcacc
     3841 tgggcggaca cctggccagg gctgctcgcc gtcgccgggc tgctggtcgt gctcggcgcg
     3901 ctggcgctgc gcgagatgcg ccgcaccgcc gcatgagcga gaccgcgcgg gcggcggtcg
     3961 ccgccgaggc cgagcgggtc ctgcgccaga actggcacga gggcgagcac gccggccgcc
     4021 gcttcgccta caccgagccg agccccggcc gctacccgtg gcagtggtac tgggactcct
     4081 gtttcgcggc gatcgtctgg cggcggtatg aggccggacg ctccaaggca gagctggaga
     4141 gcctgctcgg cgcccaggag ccgaacgggt tcgtcggcca cacgatcttc tggcgccagc
     4201 cggtcacctg gccccggctc gccttctaca acgtcgccac ccgcagcggc ttcctgcaga
     4261 cgcggacgat ccagccgccg ctgctcgcct gggcctggaa gatcgcggtc ggcgacccgg
     4321 cggcggagcc gcggatcgcc gcccaggccg actggctggc cgcccaccgc gacctcgagg
     4381 gcgacgggct gctctggatc gtgcagccgg atgagtcggg gctcgacgcc tcgccgaagt
     4441 tcgaagaggt ctggggccgg cgggccaacg ggcggatcgg cttcccgctg ctggtccgcc
     4501 gcaaccgcag gctcgggttc gacgcacggc ggatccgcga gcgcggcggg ccgctgctct
     4561 gcgaagccct cgtcaacacg ctctggtcgc tctcgctgca ggcgctgggc cgaccctcgg
     4621 cgaccccggc gctggtcgag cggctctggg acgagcggcg cggcctcttc gtcgacgagg
     4681 cgcagccggg gacgatccgg ccgcacaccg tgacctggtc ctcgctggcg ccgctggccc
     4741 tgcccgacct gccggaggcg atcgggcggc ggctggtcga ggagcacctg ctgaacgagc
     4801 gtgagttcct caccccggtg gcgccgccct cggtcgcggc caacgagccc agctacgagc
     4861 ccgacggcgg ccacggcccg atccgccgct actggcgcgg cccgacctgg gtgaactcgg
     4921 cctggctggt ctggttggga atgcgccggc tcggctacga gcgggaggcc gagcgcctcg
     4981 ccgacggcgt gatcgccgcc gccgcccgcg aaggcctgcg cgagtactac gacccgcgca
     5041 ccggcaaagg gctgggcgcc aaggacttcg cctggtctgc cctagtggcg gagctggcga
     5101 gcccggacga gggcgccgcg gcgagctatt tgtagcggta ggtgattcga cctctatcga
     5161 ggtcgtacgg cgacagctcg atcttcacgc ggtcgccggg caggatgcgg atgtagtggc
     5221 ggcgcatctt gccggagatg tggccgagga catcgtgacc gttgtccagt ttgacccgga
     5281 acatggtgtt ggggagggcc tcggtgatct ccccctccat ttcgatcttt tcctctttgg
     5341 ccatgcggcc gggaaggcta ccttaagcgg gctttttccc cggccagggt cccgtcaggc
     5401 gtcttcctcg gaccaggtcg accagttgcg ggcgtgctcg acggccgccg cgcggtcgct
     5461 cagctcgccc gcgtactggg ctgcctccag ctccgccatc gccttgccga gctcgggccc
     5521 gggctcgatt cccagctcgg cggcgagctc gtcaccgcgc aacagcgggg ccggcgggcc
     5581 ttcgcggcgc cagtcgagcg cggcggcgat catttcgcgg gcgagcgaga ggtgcgcctc
     5641 gaccatctcc ggcctggcca gcggcccggc gccgcgggcg gagaggcggt cggcgatcgt
     5701 cagcagggtg acgtcgaccc cgaccggttc ggtcgcccgc aggtagtcgt gaatgcggcg
     5761 cggcggcagc ggcgcctccg ggatcatgaa gccgagccgc agatggtgga gcgccaggcc
     5821 ctgcaggtgc tgggtgaggc ggcggctggc gcgcaggcgg gcgcagatcg tcccgatcgt
     5881 ctccgccccg accacgtcgt gaccgcggaa gccgacgaag ccaccctgtt cgctgcgcgt
     5941 cgccggcttg gcgatgtcgt gcagaagggc gccgaagcgg agcgccgtgc ggcggctgac
     6001 ctcgtcggcg agcggttctt cgagcagcgc ctcgacctcg tcggcgcgct cgccggcaaa
     6061 gcgcggcagg tcgcgctcga cctcgagcgt gtgctcgagc acggcgatcg tgtggccgtg
     6121 cacgtcgagg tggtggttcg ggccctgctc gaccccgcgc aggccctcga cctcgggcag
     6181 tacgaccgtg gtcagctcga gctcgtcgag cagcgagagg ccgcgcacgg ggttcggccc
     6241 accgagcagc tggcgcagct cggcgagctg gcgctcgccg gccgggtcgg cggcgtgcgc
     6301 ggccgccttg cgggcaagcg agacggtggc ggcgtcgacc tcgaggtcga gctgggcggc
     6361 gaagcgggcg gcgcgcagga ggcgcagcgg atcgtcttcg aagctgcgct cgctgaccac
     6421 gcgcaggagc cgcttctgca gatcggcgag gccgccgtgg ggatcgagta gctgggcggg
     6481 atcggcgagt ggggctgcca tcgccccgat cgtgaagtcg cgctcgccga ggtcggcctc
     6541 gatcgaatcg ccgcgcagca gcgtcacgtc gacctgccag cccttctggt ttgaggtgac
     6601 gcgccaggtc ccgaactcag ccgagagctc aaaggcgtgc tcgccgagac ccttgccgat
     6661 cgccttcgcg acggcgccgg ggtcgccgcc gacggcgaga tcgaggtcgg cgacctcgat
     6721 ccccagcacc gcatcgcgca ccgcgccgcc cgcgatccag acgccctcgg catcgccgag
     6781 cgcctcgcgc accgcgatca ccacaggtgc ctcggcgagc atgtcgattt tgtcaaccac
     6841 aggttgacta aattgacacc ggccgacggg ggacgcggga gctgatcccg caggtgacct
     6901 cgtagttgat cgtgtcgagg cgctgggcca cctcctcggt gcggatctcc tcctcgccct
     6961 gcctgccgat caggaccgcc tcggcgccgg gctcaaccgt ggtctcgggg ccgaggtcga
     7021 tcgtcacgtt gtccatcgag acggtgccga ccagcgggta acgacgaccg ccgaccagaa
     7081 cctcggcgtt gttggagagg gcgcggcgga cgccgtcgcc atagccgagc gggatcaccc
     7141 cgacccaggt gtcctcgggc gcctgccagc gctggccgta gccggcgctc gccccggccg
     7201 ggaagcgctt cacgtcggcg acgtaggagc gcagcgagag cgccgggacc aggccgcgct
     7261 cggcggggtc gccctggaac gggtcgagcc cgtagatcgc gacgccgcag cgggccatgt
     7321 cgaagtgggc ggcgcggtcg cggtagacgg cggcgctgtt ggcggcgtgg gcgatcacgt
     7381 tgggaaactc ggccttgacc gcctcggcga cgtcgcggaa gcgcgccagc tgctcgtcga
     7441 agaagtccga ctcgggttcg tcggcggtcg cgaagtgggt ccagaccccg gccagctcga
     7501 ggttggggtc ggcgtcgcag gcgcgggcga gcgccagcac ctcatccggg tccgggtttc
     7561 cgagccggcc catgccactg tcgtacttga cgtgcacgcg ggcgtgggcg ccctgggcgg
     7621 aggcgcgttc ggccagggcg gcgcggaagc cctcgcgcca gaccgaaacc tccgagctgg
     7681 ctgagagcac gatgtcgacc tccgccgcgg tcagcgctcc cagggtcagc aacgggatgt
     7741 gccggtagcg ccgccccacc ccctcggcct cgaccgcggt cgcgaccgcg agccaggtgg
     7801 cgccgccggc gatcgccgct ccggcgcagg cgtcggcacc gtgcccgtag ccgtcggcct
     7861 taaccaccgc gcagagttcg gttccctccc ccagctcggc cttcagcctg gcgcagttgg
     7921 cacggatcgc gtcaaagttg atgcgggcga ccgcgcgctc catctgcgcg aggttattcg
     7981 accggggcgt agcgcaggcc ggcggggatc gactcgatca cgtcggtggc gatcaccgac
     8041 tcggcggcgc cgatccgcgc ggtcgcatcg agaccggcgt gagcgtgggc gagcacggcg
     8101 gcgcaagcgg cggcaaaagg ctcgagaccg cgggcgagca gcgccgcgac gatccccgag
     8161 agcacgtccc cggtcccggc ggtggcgagc gcgggagccg agaccacgtt gacggcgacc
     8221 cgggtgccgt cggtgacgat cgtgtcgtcg cccttgagca cgacaaccgc ttccgcctcc
     8281 gcggcggcct cgcgggcggc ggcaagccgg tgagcgtcga tctcctccga gggacggccg
     8341 agcaggcggc cgagctcgcc ggcgtgcggg gtgagcacgg tcggggcggt gcgctcgccg
     8401 acccgccaga gctggccgcc aaacgagttg agcccgtcgg cgtcgatcac gagcgggcag
     8461 gtgacaccgg cggcgagctc gtgggcgagc tcgatcgtgc ccgggtcgcg ccccatcccc
     8521 ggcccgagca caccggcggc agcccgctcg aaggcagcga tcgcggcctt cgccgaggcc
     8581 ggcacgaggc agccgtcgcc gcccgggcag ccgaccgaca tcacctccgg ctggccgagt
     8641 tcgaacaccg gctccaggtc ggtcgggatc gcgaccgtgg cgtagccggc gccggcccgg
     8701 atcgccgccg aggaggacat ccgcaccgcc ccggtcagcc cgcgcgagcc gccggcgatc
     8761 gtcacctgtc cggagctgaa cttggtagag cgcgccccgc gggccggcgc cagcgccagc
     8821 accgccgggc cgatcgtgcc cgccgccgga tcgatcggcg cgccctccgg gatcccgatc
     8881 gggaccacgc gcagctcacc ggtgtgccac ttgccgggcg cgacccgctg gccgatcttg
     8941 gcggcgtgga agctgacggt gaggtcggcc tcgaccgctt ccccggcgat ctcgccgctc
     9001 gaggcgtcga cgccggaggc gatgtcgcag gcgacgaccg gcgccccgca gcggttgatc
     9061 gcggcgatcg ccgccgcggc cggttcgcgc ggcgcgccgg cgaagccggt cccgaagatc
     9121 gcgtcgacga ccgcgccgga gccggccagc caggcgtcga agtcctgcgg cgccccgtcc
     9181 tcgaagagca gcgccgtctc gacctcgtag ccgaggtcgg cgagccgccc ggcggcgatt
     9241 tcaccgtcgc cgccgttgtt gcccttgccg cagaggaccc ggatcgggcc ctgcggggtc
     9301 agctccgcga ccgcctccgc cagcgcccgt cccgccgtcg ccatcaggcc ggcggagggc
     9361 accccctgct cctcgatcgc ccagccgtcg atcgcgcgga tgccccgcgc gtcgtagagc
     9421 ggttcgagcc acggctccat cggcccagcc tacgagaccg cggcgaaggc ggcgctactt
     9481 cgagaagccg atgatcagct tcgaggtggc gaggtcgccg gaagtgccgt tgccgatggc
     9541 caggtattcg atcttctcga cgtagggccg ggcgctttcg aaatcttcgg cttcttcggg
     9601 cgagagcagg ttgtcgacca gggcgagggt ggccgggccg gcgatgtagc cggagagcgg
     9661 cgtgtcgccg agcgcgtcgc cggccttctc gaaggtcggg ttctcagcca gggtcgagcc
     9721 cttgcccgcg gtcagcgcct gggtcgaggc ggcgagtccg taggagatcg cgatcttgtc
     9781 gtctttggcg gcgaccacca gcggctggtc gccgagttcg gggtcgcgga tcgagaatcc
     9841 ggccgcgtcg ccggagagcg cggtcacgcc cggagtgttg ctggcccgca gcagcagccc
     9901 gaggttggag accgtgttgg tcgcttcctt ggcgtccttg gtggtcagca cgacggcgcc
     9961 ggtcaggttc gcctcggtgt tgccctgggc gaagacgccg aggtcgccga tcccgccggt
    10021 gagtttgtcg aggtcgatcc cgttcgcttt cagcgcgtcc ttgaattcgt tcggttcgat
    10081 ctgaccctcg atccccattt cgtcgatcag gtcgatcgat tcgccgagcc gggcaccgaa
    10141 ttcgccggcg gcgaaggcgg cgaaagaacc gccgggcaac gagccgagca gctgcgaagc
    10201 gtcgccggtc ggcgggttgt cgccggccgc gttggtcgcg aagtcgatct ccagcgattc
    10261 ggcgcccggg accacgctgg cgatcacaaa cgcttctttc ggttcgatcc cggtcgctcc
    10321 cagcagctgc tgggtttcgg cgtcgatctc ggtgcccgtt tcctcgagca cgttgccgac
    10381 gtcgacgaag acgtcggcga ggctttcgtc ggcggtcgcg tcgacggcac tgccataggt
    10441 gtcctggtcg gccagcgatt cgtcggcggc cgcgtcgaca accgccttga agatcgtttc
    10501 gtcctcggcg atgacgaagt agtcgtcgac catcccgacc gcggtgccgt cgtcttcttc
    10561 gaccttgaac gtcgtcccct cgtaggtggg ttcgtcgccg acttcggtgg agaagtcctc
    10621 gatgaattct tcggcggcgc cggcgtcggt gctttcgacc gcaatcacga cgccttcggg
    10681 atcgccaccg gcgagctcct ggaaggccat gccggcgttg tcgccgagcc acggttcgat
    10741 gtctttttcg aagtcgaggt cttcttcctc ggtgaacgcg ccttgctcga gcgccgagac
    10801 gatttcttcg ctgaggtcga cgccggcgac gttttcggcc agagcctcgg tgttcgcctg
    10861 caggtcgccc tccgggcgca cggtgacgcc gatgtagagc ggcgtctcag ccggcatcac
    10921 cgaggccggg tccgagccgc cgccgtcccc gccgccgcag ccggcgacaa ccagggcgat
    10981 ggcagcggtg gccaaggcga gaagcagaac aagacgtacc ctcatgcgac ggcgcaccct
    11041 aacaacccca ccgaccgaat tactcgaccg tgaccgtctt ggccaaatta cggggctggt
    11101 cgacgttgag gccacgggcg cgggcgatgt cgtacgcgag cagctgcagc ggcaggatcg
    11161 cgaggatcgg ctgcaggatc cagtcggtgc gggcgacggc gatcgtctcg tcggcgactt
    11221 cgccggcccg ctcggagccc tcggtggtga tcgcgatcgt ctgggcgccg cgggcgcgca
    11281 cctcctcgac gttggagagc accttctcca gcaccgggct gtcggtggcg acgcagacca
    11341 ccggggtcga ctcgtcgagc agcgcgatcg ggccgtgctt catctccccc gccgcgtagg
    11401 cgtcggtcgg cacgtaggag atctccttca gcttcagcgc gccctccagg cagatcggca
    11461 gaccgatgtg acgcccgagg tagaggaaga actcctggct gtagtgggcg gcggagaccg
    11521 ccttgaccgg ctcctcggtc atctcgagcg tctcctcgat ccgcgaaggc aggtgcttca
    11581 tttcggcgac cagctcggcg atccgctcgg ccggcagggt cccgcgcaac tcggcgagcc
    11641 gcagaccgag caggtacatc gccgccacct gggcgacgaa ggtcttggtc gcggcgacgc
    11701 tgacctccag cccggcgcgc gtgtaaagca cggcgtcggc ctcacgggtc acctggctgc
    11761 ccatgatgtt ggtgacggcg agcacggtgg caccggcctc gcgggcgagg cgcatcgccg
    11821 ccagcgtgtc ggcggtctca cccgactgcg tcatcccgac gacgaggtcg cccggcccga
    11881 ccaccgggtt gcggtagcga tactcggagg cgatgtccat ttcgaccggc acccgcgccc
    11941 actcctcgat cgcgtaacga ccgaccagac cggcgtggta gctggtcccg caggcgacga
    12001 tcacgatccg gttgaggccg cgcaggaact cgtcggagag ctcgacgtcg gagaggtcga
    12061 ccccctcgtc gccgggcagg cggtcggcga tcgtctcggc gaccgcgtcg gcctgttcgt
    12121 ggatctcctt cagcatgaag gtctcgtagc cgcccttctc ggccgcgtcg gcgtcccagg
    12181 tgacctcctc gggcgcccgc tcgaccgggt tgccctcggc gtcggagatc cgcaccccgc
    12241 ccgcttcgat ttcgacgatc tcgccgttct cgatcggcat cgccacccgc gtgtcggcga
    12301 ggaaggcggg gatcgcggag gcgaggaagg tctcaccctc gccgaccccg gcgaccagcg
    12361 ggcactcctt gcgggcaccg agcaggcgct ggggctcgtc ggcgtgcatc gcgacgaagg
    12421 cgtagtggcc gcgcagatcc gcgtaggcgg cgcgcacggc ggcggcgagg tcgccgtcgt
    12481 agtggtgggc gatcaggtgg gcgacaacct cggcgtcggt ctccgaggtg aactgctcgc
    12541 cttcggcctg gagccgcttg cgcagcgagg cgtggttctc gacgatcccg ttgagcacga
    12601 tgtggacctt gccggagctg tcgtcgtgcg ggtgcgcgtt ctcctcggtt acccggccgt
    12661 gggtggccca gcgggtgtgg gcgatacccg tattcgaagg gggcaaggca ccggcgccgt
    12721 tgtcagcgcg ggcgtcgacc gcggcgcgca ggttggcgag gttgccgacc gcgtgcacgc
    12781 tctcgacctc gccgtcctcg agcaccgaga gcccagccga gtcatagccc cggtattcga
    12841 gcttttccag tccctggagc aacagctcct cacagggcct gccgcccacg tatccgacga
    12901 ttccacacac tgaggcgaga cagagtagag ggcgcgcgcc ctcagccgag ctcttcgcgc
    12961 acgatcgcga cgaggcggcc gcagacggcc tccgcctcct cggcgctcgg cgcctcggcc
    13021 atcacccgca ccagcggctc ggtgccggag gggcgcagca gcacccgccc gcgtccggtg
    13081 agcgcctcgt tctcgcgctc gaccgcctcc cagaccgtcg ccgcgtcgtt gaccgcctcg
    13141 cggtcggcga cctcgacgtt gaccagggtc tggggcagct tctccatcgg ggcggcgtgg
    13201 tcgaggccgc gctcgccgag ctcgcgcatc gtcatcagcg ccgccgcgat cccgtcgccg
    13261 gtagcgacga agtcggtagc gatgatgtgg ccggactgct cgccgccgag ctgccagccg
    13321 cggcgcagca tctcgtcgat cacgtagcgg tcgccgacct gggtgacggc aacctcgatc
    13381 ccgtcctcgg ccatcgccgc gtggaacccg tagttggtca tcacggtgac gacgacgccg
    13441 ccgagctggc cgcgggcggc catcccacgg gcgccgagcg cgatcagctc gtcgccgtcg
    13501 tggacacggc ctgtggcgtc gaccgcgagc acgcggtcgc cgtcgccgtc gaaggcgaaa
    13561 ccgagatcgg caccgctggc gaggacctgg gaggcgagcg tctccatatg ggtggagccg
    13621 cagccggcgt tgatgttgcg cccgtcgggc tcgacgccga tcgtctcgac ccgcgccccg
    13681 agccgttcga agatcgccgg gccggcgcgg aaggtggcgc cgttggcgca gtcgaggacg
    13741 atgtggcggc cgctgaggtc gagcgggaag gccgcggtca gctcgcgcag gtagtcgtcg
    13801 aggccgccgt tcagctcgcg gaccgagccg gcgccgacgg tggccggcgg ggcctcctcc
    13861 atcagcgcct cgatctgggc ctcgagctcg tcgccgagct tcgtgccctg gccgttgaag
    13921 aacttgatcc cgttgtcctg gaacgggttg tgggaggcgg agacgaccgc ggcgaggtcg
    13981 agccccagcc gcttgaccag gatcgcggcc gccggggtcg gcagcacgcc accgagcaac
    14041 gcgtcgccgc cggccgcggc aactccggcg gccagcgccg cctccagcat cgggcccgac
    14101 tcgcgggtgt cgcggacgat caggatctgc gggcgatctg cctcgagcga agcggtgacg
    14161 gcgcgcccca gcgaggtcgc cagctcggcg ctgaggaacg tgccggcctc gccgcggatg
    14221 ccgtcggttc cgaa
//
LOCUS       DASRIG010000019         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_11565, whole genome shotgun sequence.
ACCESSION   DASRIG010000019 DASRIG010000000
VERSION     DASRIG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11565"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFB52_00420"
     CDS             complement(<1..358)
                     /locus_tag="VFB52_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZO04826.1"
                     /translation="MSSPPQRIGVVGIGYVGLPLAVAFAEQGVDVVCVDADESKLDAL
                     REGRSYIEDIPAETLAPLRERLHPSSDYAPLAECEAVIVCVPTPLTNSREPDLTYLLG
                     AAEGLAAVLKAGQLVVL"
     gene            437..961
                     /locus_tag="VFB52_00425"
     CDS             437..961
                     /locus_tag="VFB52_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZO04827.1"
                     /translation="MTDTTQEFTTDQAEREASGAHSYGRYLEEFEVGAVYKHWPAKTV
                     TEADDHLFCLITMNHHPLHINDVYAGNSQQGRNVVVGPLVYSLALGMSVSDVSGKAIA
                     NLATEELSHPNPVFHGDTLFCETEVLEVKPSKSKLDRGVVKVHTRVLNQDGVLVAEFK
                     RLVLVPRKEHGTPE"
     gene            complement(976..2118)
                     /locus_tag="VFB52_00430"
     CDS             complement(976..2118)
                     /locus_tag="VFB52_00430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO04828.1"
                     /translation="MEFERLSVEALPELPAPNKEWLVLGHAAGNPFTTWEWASAWWRE
                     FGEERRQLILGCRDESGELIGLLPLFAASERPARVLRFVGHGAADQLGPVCRPEHREA
                     VAAAFLGALRERRDWDICMAERLATVEAWPELIGGTVTRSERSPTIHVKTTDWTEFLS
                     NYSSNFRGQVRNFENRLAKKHELAFRRSEDPERLDDDLDVFFRLHEQRWENGAGGSTV
                     FSSGLEQFHRGFAHEALRRGWLRLSFLELDGEPVASAYVFRLGGADWLYQQGWDPSLA
                     KQRVGRVMLNRTIRDAVEDGMSEYKFLLGEEEYKDRYTDEDAPVTTVAIGRNAVFRSA
                     VGAAVPLKRMVANARDAARRTLARGTPAFPVFDEMLAGGVFAPAGI"
BASE COUNT          370 a          804 c          811 g          333 t
ORIGIN      
        1 cgagcacaac cagctggccc gctttcagca ccgcggcgag gccctcggcg gcgccgagca
       61 ggtaggtgag gtccggttcg cgcgagttgg tcagcggcgt cggcacgcag acgatcaccg
      121 cctcgcactc ggccagcggc gcgtagtcgg agctggggtg gaggcgctcg cgcagcggag
      181 cgagcgtttc ggcggggatg tcctcgatgt agctgcggcc ctcgcgcagg gcgtccagct
      241 tcgactcgtc ggcgtcgacg caaaccacgt ccacgccctg ctcggcgaag gcgacggcga
      301 gcgggagacc gacgtagccg atgccgacca caccgatcct ctgcggcggc gaactcaccc
      361 gcgggagtct atgggcggcc tccgtcgcca cgcggatagc atccccgccg ccaaacaatc
      421 aggagaagac tcacccgtga ccgatacgac tcaggagttc accaccgacc aggcagagcg
      481 cgaggcctcc ggcgcccaca gctacgggcg ctacctggag gagttcgagg tcggcgccgt
      541 ctacaagcac tggccggcga agaccgtgac cgaggcggac gaccatctgt tctgcctgat
      601 cacgatgaac caccacccgc tgcacatcaa cgacgtctac gccggcaact cccagcaggg
      661 ccgcaacgtc gtcgtcgggc cgctcgtcta ctcgctggcg ctcgggatgt cggtctccga
      721 cgtctccggc aaggcgatcg ccaacctggc gaccgaagag ctgagccacc cgaacccggt
      781 cttccacggc gacacgctgt tctgcgagac cgaggtgctg gaagtgaagc cgtcgaagtc
      841 gaagctcgac cgcggcgtgg tcaaggtcca cacccgcgtg ctgaatcagg acggcgtgct
      901 cgtcgccgag ttcaagcgcc tggtcctggt cccgcgcaaa gagcacggca cgccggagta
      961 gcgcgcgagc gaggctcaga tgccggcggg ggcgaagacc ccgccggcga gcatctcgtc
     1021 gaacaccggg aacgccgggg tgccgcgggc gagcgtcctg cgcgccgcgt cgcgagcgtt
     1081 cgcgaccatc cgtttcagcg gcacggcagc gccgaccgcc gagcggaaga ccgcgttgcg
     1141 cccgatcgcg accgtggtca ccggcgcgtc ctcgtcggtg tagcggtcct tgtactcctc
     1201 ttcgccgagc aggaacttgt actcgctcat gccgtcctcg accgcgtcgc ggatcgtccg
     1261 gttgagcatc acgcgaccga cccgctgctt ggccaggctc gggtcccagc cctgctggta
     1321 gagccagtcg gcgccgccga ggcggaagac gtaggcggag gcgaccggct cgccgtcgag
     1381 ctcgaggaag gagagccgca gccagccacg ccgcagcgct tcgtgggcga acccgcggtg
     1441 gaactgctcg aggccgctgc tgaagacggt cgagccaccg gcgccgtttt cccagcgctg
     1501 ctcatgcagg cggaagaaga cgtcgaggtc gtcgtcgagt cgctcgggat cctcgctgcg
     1561 gcggaaggcg agctcgtgtt tcttcgccag gcggttctcg aagttgcgca cttgcccgcg
     1621 gaagttggag ctgtagttgg agaggaactc ggtccagtcg gtggtcttga cgtggatcgt
     1681 cgggctgcgc tcgctgcggg tgacggtgcc gccgatcagc tccggccagg cctcgactgt
     1741 ggccagccgc tcggccatgc agatgtccca gtcgcggcgc tcgcgcagcg ctcccaggaa
     1801 ggccgcggcg accgcctcgc ggtgctcggg ccggcagacc gggccgagct ggtcggcggc
     1861 cccgtggccg acgaagcgga gcacgcgggc gggccgctcg gaggcggcga agagggggag
     1921 gaggccgatc agctcgccgc tctcgtcgcg gcagccgagg atcagctggc gccgttcctc
     1981 gccgaactcg cgccaccagg cgctcgccca ctcccaggta gtgaacggat ttcccgccgc
     2041 gtgaccgagc accagccact ccttgttcgg ggccggcagc tcgggcagcg cctccacgct
     2101 gagccgctca aattccatct cgaaaaccgt atcggcagcc ggcgatagat gcattaggga
     2161 tgccttaccg gcgcccgaat tgcagcgagc gcagaccgcg gcgcaggggc agcagcgccg
     2221 ccagggccgc gtcggcgagg acgccgcgac cgaaggcgac ggtttccacc ggcgcgtctt
     2281 catcgctgta gtggttcttg tactcctctc cgccgagc
//
LOCUS       DASRIG010000020         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2096302, whole genome shotgun sequence.
ACCESSION   DASRIG010000020 DASRIG010000000
VERSION     DASRIG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096302"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFB52_00435"
     CDS             complement(<1..310)
                     /locus_tag="VFB52_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04829.1"
                     /translation="MLAFAIGQLVHALGETLGGAAVVHEDDRGGVFLDQLGQLRVDRR
                     PNRANMRRRLYFSRRQPVLRGIRRSPRVRHVLDRHHDLQIQRLARPRINDLASPLRPD
                     Q"
     gene            482..>2454
                     /locus_tag="VFB52_00440"
     CDS             482..>2454
                     /locus_tag="VFB52_00440"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZO04830.1"
                     /translation="MLKSGELPCLVATSSLELGIDMGAVDLVIQVESPKSVTRGLQRI
                     GRAGHGLGETSKGRIFPKYRGDLLECAVVARRMREGEIEETTIPQNPLDVLAQHLVSM
                     AALDEWKIDEVEALVTATESFRELSREQLENVLDMLDGRYPSDRFAELRPRIVWDRTG
                     GTMHGRKGSRQLVVTNAGTIPDRGLYGVHLPDGRRVGELDEEMVYEARPGQTFLLGAS
                     TWRIEEITRDRVIVIPAPGAPGAVPFWRGDGIGRPAELGRAIGEFSREAVSREPAELA
                     KEYDLDPRAANNLVSYLREQQQATRVVASDETIVVERFRDEIGDWRLCILSPFGGRVH
                     SAWGLALAAKLRQEHGLEADAIWSDDGIVIHLPDADEPPSADLVLIEPDEIEDLIVSE
                     LSGSALFGARFRENASRSLLIPRAYPGKRTPLWQQRLKSQSLLEVARDFPRFPVILET
                     YRECLQDVLDLPALRDLLGRLQARSLSLVEVETPTASPFASSLLFDYVATYMYEGDTP
                     NAERRAAALALDRDLLRELLGQEELRELIDPEALEDVEAQLQHRTEAGRAGDRDALQQ
                     VLRNLGDMTAEEAAERVAEGYSAKSMLEKLVGERRVALVRIAGEERYIAAEDAGLYRD
                     ALGVPPPAGLPETFLEEHPDAMRALVRRFARTHG"
BASE COUNT          370 a          910 c          827 g          347 t
ORIGIN      
        1 cctggtcggg ccgaagcgga cttgcgagat cgttgatgcg gggacgcgca agccgctgga
       61 tctggagatc gtggtgcctg tcgaggacat ggcggacccg ggggctccgg cgtatccctc
      121 tgaggacggg ttgccgccga ctgaagtaga gccgtcggcg catattcgct cgatttggcc
      181 ggcgatctac ccgaagctgg ccgagctggt ccaggaacac acctccacga tcatcttcgt
      241 gaacaaccgc cgcgccgccg agcgtctcgc caagcgcatg aacgagttgg ccaatggcga
      301 aggcgagcac gagcttccag ctacggagca cgcggggcat ggaaacgggt ccggggggga
      361 ccctccgtca caaattagtg gaggttcctc cgggtccccc ccggacccgt cgccatacgt
      421 tgagattgct cgtgcgcacc atggatcgct ttcccatgag gagcgcactc tggtcgagga
      481 gatgctgaag tcgggggagc tgccgtgcct cgtggcgacc tcgtcgctgg agctcggcat
      541 cgacatgggc gccgtcgacc tggtgatcca ggtcgagtcg ccgaagtcgg tgacccgcgg
      601 attgcagcgg atcgggcgcg ccgggcacgg gttgggcgag acctcgaaag gccggatctt
      661 ccccaaatac cgcggcgacc tgctcgagtg cgcggtggtc gcgcggcgca tgcgcgaagg
      721 cgagatcgag gagacgacga tcccgcagaa cccgctcgac gtgctcgccc agcacctggt
      781 ctcgatggcc gcgctcgacg agtggaagat cgacgaggtc gaggccctgg tcaccgcgac
      841 cgagtcgttc cgcgagctct cccgcgagca gctcgagaac gtgctcgaca tgctcgacgg
      901 ccgctacccc tccgaccgct tcgccgagct gcgtccgcgg atcgtctggg accggaccgg
      961 cggcaccatg cacgggcgca aaggctcccg gcaactggtc gtcaccaacg ccggcacgat
     1021 ccccgaccgc ggcctctacg gcgtccacct gcccgacggc cgccgggtcg gcgagctcga
     1081 cgaggagatg gtctacgagg cgcgcccggg ccagaccttc ctgctcggcg ccagcacctg
     1141 gcggatcgaa gaaatcaccc gcgaccgggt gatcgtgatc cccgctcccg gcgcccccgg
     1201 cgcggtcccg ttctggcgcg gcgacgggat cggccgcccc gccgagctcg gccgcgcgat
     1261 cggcgagttc tcccgcgagg cggtctcccg cgagcccgcc gagctcgcca aggagtacga
     1321 cctcgacccg cgcgccgcca acaacctcgt ctcctacctg cgcgagcagc agcaggcgac
     1381 ccgcgtcgtc gcctccgacg agacgatcgt ggtcgagcgc ttccgcgacg agatcggcga
     1441 ctggcggctc tgcatcctct ccccgttcgg cggccgcgtc cactccgcct ggggattggc
     1501 acttgccgcc aagctgcgcc aggagcacgg cctcgaggcc gacgcgatct ggtccgacga
     1561 cggcatcgtc atccacctcc ccgacgccga cgagccgccc tctgccgacc tcgtgttgat
     1621 cgagcccgac gagatcgagg acctgatcgt ctccgagctc tccggcagcg ccctgttcgg
     1681 cgcccgcttc cgcgagaacg cctcgcgctc gctcttgatc ccgcgcgcct accccggcaa
     1741 acgcacgccg ctctggcagc agcgcctcaa gtcccagagc ctgctcgagg tcgcccgcga
     1801 cttcccccgc ttcccggtca tcctcgagac ctaccgcgag tgcctgcagg acgtgctcga
     1861 cctgccggcg ttgcgcgacc tgctcggccg cctgcaggcg cgctcgctca gcctggtcga
     1921 ggtggagacg ccgaccgcct caccgttcgc ctcctcgctg ctcttcgact acgtcgccac
     1981 ctacatgtac gagggggaca cgccgaacgc cgagcggcgg gcggcggcgc tggccctcga
     2041 ccgcgacctg ctgcgcgagc tgctcggcca ggaggagctg cgcgagctga tcgaccccga
     2101 agcgctggag gacgtcgaag cgcagctgca gcaccgcacc gaggccggcc gcgccggcga
     2161 ccgcgacgcg ctccagcagg tgctccgcaa cctcggcgac atgaccgccg aggaggccgc
     2221 cgaacgggtc gccgagggct actcggcgaa gtcgatgctg gagaagctgg tcggcgagcg
     2281 ccgcgtcgcc ctggtccgga tcgccggcga ggagcgctac atcgccgccg aggacgccgg
     2341 cctctaccgc gacgcgctcg gggtgccgcc gcccgccggg ctgcccgaaa ccttcctcga
     2401 ggagcacccg gacgcgatgc gcgccctggt ccgccgcttc gcccgcaccc acgg
//
LOCUS       DASRIG010000021         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1321983, whole genome shotgun sequence.
ACCESSION   DASRIG010000021 DASRIG010000000
VERSION     DASRIG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321983"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFB52_00445"
     CDS             complement(<1..872)
                     /locus_tag="VFB52_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04831.1"
                     /translation="MKAWWGIVAAACALALSLPAAATAAQTEVQRPVVSASLYAGIDN
                     GYLILVGFPSPKIAALYLLRIDRGETRALRTASYVVRARSRLVNGVLRADFGKIGSVK
                     ARFVPSGKTRRGRIQRGCTGPRPILEGGEFRGTVKLRGEDGYFQVAARSGAAQRERSF
                     RLVCRPGWAFNLPPGLTLRSYLLPNFRLVFTGNGGNIAQLRAAAKIDGRSISLLASHG
                     SGAPAGAEVEVSTVESANGMAIGRTLSVDGGEGTLLTSLPGVRPGSATLAPPAPFHGE
                     AQYTENSSTSHTWSG"
     gene            942..1655
                     /locus_tag="VFB52_00450"
     CDS             942..1655
                     /locus_tag="VFB52_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO04832.1"
                     /translation="MDSLDGRVIAIAGVGGGLGPLVAERLAEAGATVAGNDRDQGALD
                     ALGERLGLGPERWDGRVVDLLDEEAARAWCAALVERFGRVDGLVHLVGGWRGGEPLHE
                     ASLEDWDFLHGLLVRTVQHTTRAFHDQIAASSAGRFVLVSAKQAQEPSNTNAAYAAAK
                     AAAEAWTLAFADGFEPGGATANIVVVDAILTPRMREQNPDGDFATFTPAEHLAEGIAF
                     LCSDAAAEMNGQRLPLTMR"
     gene            1655..2458
                     /locus_tag="VFB52_00455"
     CDS             1655..2458
                     /locus_tag="VFB52_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO04833.1"
                     /translation="MSTAQRRGKAPDGKMTIGELARRTGMTVRNIRAHQTRGLLPPPE
                     VQGRTGYYNEEHVARIELTREMQAEGLNLEAIRRVLESTGGSSQEMVDFARAVRAPFE
                     DETPEIFEAKELAAAWQVKELDPKLIRHGERLGILRELPDGKIEAISPRLHRASQALV
                     EFGVGPEAGMALADKLRNHAEGVARVYVDLFVKEIWEPFDRAGRPEDEWPRVLEALEQ
                     LRPLASDALVAMFQITMGEATEKAAERTLHDAGKPARKGARKRRSRSAS"
     gene            complement(2427..3122)
                     /locus_tag="VFB52_00460"
     CDS             complement(2427..3122)
                     /locus_tag="VFB52_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZO04834.1"
                     /translation="MAAVLAFAAAPAQALNTRVSIANYAWSKPQVQIDLNEKVTWDWL
                     GPDLAHSVTGISANDLQWDSDPGTDAPSHQAGDSYTLQFSQPGTYLFQCKLHASVRGE
                     VIVSGVPGNPDSDPGPQPPLNVDVKPPTLGSLVIKSQRFKGTKGVAMSAQISDRGILD
                     AEYYRFNSKGKRVYNGYKTWQTFIGVNHVKLGARSKHFRAKPGRYEVVLRATDTANNV
                     SKPIKKRFEIVAS"
BASE COUNT          478 a         1074 c         1178 g          479 t
ORIGIN      
        1 ccgctccagg tatgcgaggt cgacgagttc tccgtgtact gcgcttcgcc gtggaacggg
       61 gcgggtggcg ccagcgtggc cgagccggga cggacgccgg gcaggctggt cagcagcgtg
      121 ccctcgccgc cgtcgaccga gagggtgcgg ccgatcgcca tcccgttggc ggactcgacc
      181 gtgctcactt cgacttcggc gccggccggt gcgccgctcc catgcgaagc cagcagcgag
      241 atcgagcgcc cgtcgatctt cgccgccgcc cgcagctggg cgatgttgcc gccgttgccg
      301 gtgaagacga ggcggaagtt gggcagcagg tagctgcgca gggtcaggcc cggcggcagg
      361 ttgaacgccc acccggggcg gcagacgagc cggaaggagc gttcgcgctg ggccgcgccc
      421 gatcgcgccg ccacctggaa gtagccgtcc tcgccgcgca gcttgacggt gccgcggaat
      481 tcgccgcctt cgaggatcgg ccgcggtccg gtgcagcccc gttggatgcg tccccgccgg
      541 gttttaccgc tgggcacgaa gcgcgccttg acgctgccga tcttgccgaa gtcggcgcgc
      601 aggaccccgt tgacgaggcg gctgcgggcg cgcacgacgt agctggcggt gcgcagggcg
      661 cgggtctctc cccggtcgat gcgcaggagg tagagcgcgg cgatcttcgg gctcgggaag
      721 ccgaccagga tgaggtagcc gttgtcgatg ccggcgtaga gactggcgga gaccactggc
      781 cgctggactt cggtctgcgc ggccgttgcg gcagccggca gggagagcgc cagtgcgcac
      841 gccgccgcca cgatccccca ccatgccttc atcgccccag actatttgaa cggcgctgta
      901 ctgctgtgta gaacggatta gggaaagatg taggtgtggg agtggattcg ctcgatggac
      961 gtgtgatcgc gatcgccggg gttggcggcg gccttgggcc gctggtcgcc gagcggctgg
     1021 cggaggccgg tgccaccgtc gccggcaacg accgcgacca gggcgcgctc gacgcgctcg
     1081 gcgagcgact cggcctcggt cccgagcgct gggacgggcg cgtcgtcgac ctgctcgacg
     1141 aggaggcggc gcgcgcctgg tgcgcggccc tggtcgagcg cttcggccgg gtcgacggcc
     1201 tcgtccacct ggtcggcggc tggcgcgggg gagagccgct gcatgaggcc tcgctcgagg
     1261 actgggactt cctccacggc ctgctcgtgc gcaccgtcca gcacacgacc cgcgcctttc
     1321 acgaccagat cgccgccagc tccgccggcc gctttgtcct cgtctccgcc aagcaggcgc
     1381 aggagccgag caacaccaac gccgcctacg ccgccgccaa ggctgccgcc gaggcctgga
     1441 cgctggcctt tgccgatggc ttcgagcccg gcggcgcgac cgccaacatc gtcgtcgtcg
     1501 acgcgatcct gacgccgcgg atgcgcgagc agaatcccga cggcgacttc gccaccttca
     1561 ccccggccga gcacctcgcc gaggggatcg catttctctg ctcagacgcg gcggcggaga
     1621 tgaacgggca gcgactgccg cttacgatgc gctgatgagc accgcgcaga ggcgggggaa
     1681 ggcccccgac gggaaaatga cgatcggcga gctggcgcgg cggaccggga tgaccgtgcg
     1741 caacatccgc gcccaccaga cccgcggcct gctgccgccg ccggaagtcc agggccgcac
     1801 cggctactac aacgaggagc acgtcgcgcg gatcgagctg acccgcgaaa tgcaggccga
     1861 gggcctcaac ctcgaggcga tccggcgggt gctggagagc accggcggct cctcccagga
     1921 gatggtcgac ttcgctcgcg ccgtgcgcgc cccgttcgag gacgagactc cggaaatctt
     1981 cgaggccaag gagctggcgg cggcctggca ggtgaaggag ctcgacccga agctgatccg
     2041 ccacggtgag cggctcggca tcctgcgcga gctccccgac ggcaagatcg aggcgatcag
     2101 cccgcgcctg caccgggcca gccaggcact ggtcgagttc ggggtcgggc cggaagcggg
     2161 gatggcgctc gccgacaagc tgcgcaacca cgccgaaggg gtcgcccgcg tttacgtcga
     2221 tctcttcgtc aaggagatct gggagccgtt cgaccgcgcc ggtcgccccg aggacgagtg
     2281 gccgcgggtg ctcgaggcgc ttgagcagct gcggccgctc gcctccgacg cgctggtcgc
     2341 gatgttccag atcacgatgg gcgaggcgac cgaaaaagcg gcggaacgga ccctccacga
     2401 cgccggcaaa ccggcacgca agggcgctag gaagcgacga tctcgaagcg cttcttgatc
     2461 ggcttggaga cgttgttggc ggtgtcggtc gcccgcagca ccacctcgta gcgccccggc
     2521 ttggcccgga agtgcttgga gcgggcgccg agcttgacgt ggttgacacc gatgaaggtc
     2581 tgccaggtct tgtagccgtt gtagacgcgt ttgccctttg agttgaagcg gtagtactcg
     2641 gcgtcgagga tgccgcggtc gctgatctgg gcgctcatcg cgacgccctt ggtccccttg
     2701 aagcgctggc tcttgatcac caggctgccg agcgtcggcg gcttcacgtc gacgttcagc
     2761 ggcggctgcg gtcccgggtc ggagtcgggg ttgccgggaa ccccggagac gatcacttct
     2821 ccgcgcaccg aggcgtgcag tttgcactgg aagaggtagg tccccggctg gctgaactgc
     2881 agcgtgtagc tgtcgccggc ctggtgggag ggggcgtcgg tgccggggtc ggagtcccac
     2941 tgcaggtcgt tggcggagat cccggtgacc gagtgggcga ggtcgggccc gagccagtcc
     3001 caggtcacct tctcgttcag gtcgatctgc acctgcggct tcgaccaggc gtagttggcg
     3061 atcgagaccc gcgtgttcag cgcctgcgcc ggggcagccg cgaacgccag gaccgccgcc
     3121 acgaggagcg ctgtcgaggt tagtggcgct attcgccagg aacctcgaca acaccgggct
     3181 aggtgacgag gtaccaaccg ttcatcccc
//
LOCUS       DASRIG010000022         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_12724, whole genome shotgun sequence.
ACCESSION   DASRIG010000022 DASRIG010000000
VERSION     DASRIG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12724"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFB52_00465"
     CDS             <1..465
                     /locus_tag="VFB52_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04835.1"
                     /translation="GIVAEIGAMRISDEIDALEVMGVPPVTFLCASRLLAAWMALPFI
                     YLVSIGIMYGASYLAVVEQVADVSAGGYELIFWMFQDPTDVVFSLAKGMVMATVIVLV
                     GCYYGYTASGGPVGVGTATAKSMVFNIVAVHILGMLGTLIFWGANPRMPIGG"
     gene            complement(470..643)
                     /locus_tag="VFB52_00470"
     CDS             complement(470..643)
                     /locus_tag="VFB52_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04836.1"
                     /translation="MRLTPHQRMALRERQTRLVMDVVVRVFAMAYLVAILIVAPLMLA
                     PTGFAAAAYLWRR"
     gene            735..1046
                     /locus_tag="VFB52_00475"
     CDS             735..1046
                     /locus_tag="VFB52_00475"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO04837.1"
                     /translation="MDEVFKALADPTRRQLLDALHERDGQTLSELERPLEMSRFGVMK
                     HLRVLDEAGLITTRKRGREKLHFLNPVPIRLVYERWVSKYEEPWASALTELKREIEEE
                     Q"
     gene            1046..1552
                     /locus_tag="VFB52_00480"
     CDS             1046..1552
                     /locus_tag="VFB52_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO04838.1"
                     /translation="MRDEEKAYAVVGGRGSMTVFEIYIKTTPERLWEAITDPELRAKY
                     SFGVGVESDWTPGSAYKASVPGVIEISSGENVEVDPPRLLVQTFTAHWSEGVESEGVS
                     RVTWEIEPVGDSCCLRVTHDQLGEDANSELFGGWPMILSGLKTLLETGELLDTPGSLR
                     YSGASEWS"
     gene            complement(1525..2202)
                     /locus_tag="VFB52_00485"
     CDS             complement(1525..2202)
                     /locus_tag="VFB52_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HZO04839.1"
                     /translation="MNRSRSIAFAGLGAALVCNYWLLEWWLSERTDTPRSWISDLATR
                     SEGTGWRFQLLAILSGLAISAFAVLLLRRDRGALRRGLLALLATGAFATVAGAAPLSC
                     PEGIEPSCTLAEDPVDILHALATGGEIIATVLAFLLIGLALSRSVLLLSPRGRQSAYG
                     QRLGAVTLGIGVVWLVLTAATALAYLSGDVDAVKGVLQRASQVLFGTWLALLGFWFAQ
                     DHSEAPE"
     gene            2253..>3488
                     /locus_tag="VFB52_00490"
     CDS             2253..>3488
                     /locus_tag="VFB52_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO04840.1"
                     /translation="MSGNGPGAKSQERGNLDAILTTAEHDRKRLNSNALGPFSLTLLG
                     IASVVGAGIFAVTGIAAAEHAGPAVVISFVLAGIVAGLTALCYAELASMIPAAGSTYS
                     YALAAFGSFIGWFIGWDLLLEYLFAASTVAVSWSSYFVDLLGSVGIQIPHDLANPPFG
                     DDPGIVNLPALAIMLLTSAALWAGTRESAIANNSMVLLKLSILALLVAVGAFYVISSN
                     YEPFVPAAGGGFGDFGWSGILRGAGVVFFAYIGFDAVATAAAEARNPKRTVPIGLLGT
                     VIIATAIYVAVGAVLVGMVNYRELGVGDPIAVALEAGGRDLDWLLKLTDIAAVVGLAS
                     TVLVTLYGQTRIFMRMSEDGMLPPAFGKVGAKTKTPHFSIIVCGVAGGIVAALVPLSV
                     LGELISIGTLLAFIIVSSGV"
BASE COUNT          544 a         1195 c         1182 g          567 t
ORIGIN      
        1 gggatcgtcg ccgagatcgg cgcgatgcgg atctccgacg agatcgacgc gctcgaagtg
       61 atgggcgtgc cgccggtcac cttcctctgc gccagccgcc tgctcgccgc ctggatggcg
      121 ctgccgttca tctacctggt ctcgatcggg atcatgtacg gggcgagcta cctggcggtg
      181 gtcgagcagg tcgccgacgt ctcggccggc ggctacgaac tgatcttctg gatgttccag
      241 gaccccaccg acgtggtctt cagcctcgcc aagggcatgg tgatggcgac cgtgatcgtg
      301 ctggtcggct gctactacgg ctacaccgcg tccggcggcc cggtcggcgt cggcaccgcg
      361 accgcgaagt cgatggtctt caacatcgtc gccgtccaca tcctcggcat gctcggcacc
      421 ctgatcttct ggggagccaa cccgcgcatg ccgatcggcg gctgagcggc tagcgacgcc
      481 agaggtaggc ggctgcggcg aacccggttg gcgccagcat cagtggggcg acgatcagga
      541 tcgcgaccag gtacgccatc gcgaagacgc ggacgacgac gtccatgacc aggcgcgtct
      601 gccgctcgcg cagcgccatg cgctggtgcg gggtcaagcg catggggtgg acgatagaca
      661 tatggaagcg ttttgtctgc aaccttcagt ctcccggttg acatgtgacc aaaaggtcac
      721 ctatagtccg ttccatggac gaggtgttca aggcgcttgc ggacccgacc cggcggcagt
      781 tgctcgatgc cctgcacgag cgggacggcc agaccttgag cgagctcgag cggccgctgg
      841 agatgtcgcg cttcggcgtg atgaagcacc tcagggtgct ggatgaggca ggtctgatca
      901 ccacccgcaa gcgcggccgc gagaagctcc actttctcaa ccccgtcccg atccggctcg
      961 tctacgagcg ctgggtcagc aagtacgagg agccctgggc ctcggcgctc acggagctca
     1021 aacgagagat cgaggaggag cagtgatgag ggacgaagag aaggcctacg cggtggtcgg
     1081 cgggcgcggc tcgatgaccg tgttcgagat ctacatcaag acgacgccgg agcggctctg
     1141 ggaggcgatc accgatcccg agctgcgcgc caagtacagc ttcggggtcg gtgtcgagtc
     1201 cgactggacg ccgggctcgg cctacaaggc gagcgtgccg ggggtgatcg agatctcctc
     1261 cggggagaac gtcgaggtcg acccgccgcg cctgctggtc cagaccttca ccgcccactg
     1321 gagcgagggg gtcgagtccg aaggggtctc gcgggtgacc tgggagatcg agccggtcgg
     1381 cgactcctgc tgcctgcgcg tcacccacga ccagctcggc gaggacgcca atagcgagct
     1441 gttcggcggc tggccgatga tcctctccgg cctcaagacc ctgctggaga ccggcgagct
     1501 gctcgacacc cccggctcgc tgcgctactc cggcgcctcc gagtggtcct gagcgaacca
     1561 gaagccgagt aacgccagcc aggtcccgaa gaggacctgg ctggcccgct gcaggacccc
     1621 cttgaccgcg tccacgtccc cgctcaggta ggcgagcgcc gtggctgcgg tcagcaccag
     1681 ccaaaccacc ccgatcccca gcgtcacagc ccccagacgc tgaccgtatg cactttgtcg
     1741 ccctctgggc gacaaaagga gtacggagcg gctgagggcg aggccgatga ggaggaaggc
     1801 gaggacggtg gcgatgattt cgccgccggt ggcgagggcg tggaggatgt cgacggggtc
     1861 ctcggcgagg gtgcagcttg gttcgatgcc ttccgggcag ctgagcgggg cggcgccggc
     1921 gaccgtcgcg aaagcgccgg tcgcgagcag ggcgagcagg ccgcggcgaa gcgctccgcg
     1981 gtcgcgccgc agcagcagca ctgcgaaggc gctgatcgcc agcccggaga ggatcgcgag
     2041 cagctggaag cgccagccgg tgccctccga gcgcgttgcc aggtcgctga tccaggagcg
     2101 cggggtgtcg gttcgttccg acagccacca ttcgagcagc cagtagttgc agacgagcgc
     2161 ggcgccaagg cccgcgaacg cgatcgaccg agagcggttc acaggcagcg cacactaaaa
     2221 ggagccgggc ctctgaggca gaatttcgct ggatgagcgg aaacggcccg ggcgccaagt
     2281 cgcaggagcg cggcaacctc gatgcgatcc tgacgaccgc cgagcacgac cgcaaacggc
     2341 tcaactcgaa cgccctcggt cccttcagcc tcaccctgct cgggatcgcc agcgtggtcg
     2401 gcgccgggat cttcgcggtc accggcatcg ccgcggccga gcacgccggg ccggcggtgg
     2461 tgatctcctt cgtcctcgcc gggatcgtcg ccggactcac ggcgctctgt tacgccgagc
     2521 tcgcctcgat gatcccggcc gccggcagca cctactccta cgcccttgcc gccttcggct
     2581 ccttcatcgg ctggttcatc ggctgggacc tgctgctcga gtacctgttc gccgcctcga
     2641 ccgtcgccgt cagctggtcc tcctacttcg tcgacctgct cggctcggtc ggcatccaga
     2701 tcccgcacga cctcgccaac ccgcccttcg gcgacgaccc cgggatcgtc aacctgccgg
     2761 ccctggcgat catgctgctg acctcggcgg cgctctgggc cgggaccagg gagtcggcga
     2821 tcgccaacaa ctcgatggtc ctgctcaagc tctcgatcct cgcgttgctt gtcgcggtcg
     2881 gcgccttcta cgtgatctcc tccaactacg agccgttcgt tcccgccgcc gggggcggtt
     2941 tcggcgactt cggctggagc ggcatcctgc gcggcgccgg ggtcgtcttc ttcgcctaca
     3001 tcggcttcga cgcggttgcg accgccgccg ccgaggcgcg caacccgaaa cgcaccgtgc
     3061 cgatcggcct gctcggcacc gtgatcatcg cgaccgcgat ctacgtcgcc gttggtgcgg
     3121 tgctggtcgg gatggtcaac taccgcgaac tcggggtcgg cgacccgatc gcggtcgcgc
     3181 tcgaagccgg cggtcgcgac ctcgactggc tgctcaagct gaccgacatc gccgccgtcg
     3241 tcggcctcgc ctcgaccgtg ctcgtcaccc tctacgggca gaccaggatc ttcatgcgga
     3301 tgtcggagga cgggatgctg ccgccggcct tcggcaaggt cggcgccaag accaagacgc
     3361 cgcacttctc gatcatcgtc tgcggcgtcg ccggcggcat cgtcgccgcc ctggtgccgc
     3421 tctcggtgct cggcgaactg atctcgatcg gcaccctgct ggccttcatc atcgtctcct
     3481 ccggcgtc
//
LOCUS       DASRIG010000023         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_272223, whole genome shotgun sequence.
ACCESSION   DASRIG010000023 DASRIG010000000
VERSION     DASRIG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272223"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="aguA"
                     /locus_tag="VFB52_00495"
     CDS             complement(<1..1015)
                     /gene="aguA"
                     /locus_tag="VFB52_00495"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HZO04841.1"
                     /translation="MSATLDSTPAADGFRMPGEFEPHDGCWMAWPERADNWRLGAEPA
                     QRAYAAVAAAIAPSEPVTMAVSEAQFERARELLPAAVRVVEVALDDAWMRDTGPTFVI
                     DGAGHRRGIDWRFNAWGGHHGGLYESWDRDELVAAKVLEVEGADRYPAPLVMEGGAIH
                     VDGEGTVLTTEQCLLNRNRNPQLSRAQIEAALCAYLGAEKVVWLGQGVHEDETDGHVD
                     NLACFAAPGVVVLTWTDAERDPQHAISRDARERLEAATDARGRPFEVVLLPSPGPLLT
                     TAEEAAGVEPVPGTKPRRGGDRLAASYANFYLGTSRVVFPLLDQRTDEEAAEILRRCF
                     PDRE"
     gene            1061..1999
                     /locus_tag="VFB52_00500"
     CDS             1061..1999
                     /locus_tag="VFB52_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZO04842.1"
                     /translation="MQLLTAYEVLDSPARTRPADREPFRVGAVQERWHEDPAEHRAAL
                     AAGVELAAGEGARLVCLQELTLSPYFAVDPAGAAAAGVEPESLPGGPTFELAAELARA
                     HGIHVHASLFERTEDGEGDGRGFNTAILVAPDGELAARTRKLHIPVTAGYYEDRYFLP
                     GPATGDPYPVIALDQARLGLPTCWDQWFPELARSYSLAGAEVLVYPTAIGSEPDHPGF
                     DTQPLWQRVIVANGIANGLFMVAVNRTGSEPPLAFYGSSFISDPYGRVLVEAPRDEPA
                     VLVADLDLDQRRDWLELFPFLTTRRPDAYGSLVDPG"
     gene            complement(1972..2430)
                     /locus_tag="VFB52_00505"
     CDS             complement(1972..2430)
                     /locus_tag="VFB52_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO04843.1"
                     /translation="MPSAEQPLTGRCACGTVRFRVTEPFLSAGYCHCERCQHRSGVPW
                     TFNGLVDASGFELTAGAGEVGNWVPDGGGRPKSFCRRCGGHVFGGDPEGGELIAVRLG
                     AVEGDPEVEPSWRAWVSSAPAWYPIPDDGLPRFPEGAGSARTQPGSTSEP"
     gene            2568..2753
                     /locus_tag="VFB52_00510"
     CDS             2568..2753
                     /locus_tag="VFB52_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04844.1"
                     /translation="MSHKSWTFTTYENGKPVATESNLDSRQALAAIRAAMVGEPVAPV
                     AQHRREVAEPVELAHAA"
     gene            complement(2785..>3358)
                     /locus_tag="VFB52_00515"
     CDS             complement(2785..>3358)
                     /locus_tag="VFB52_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZO04845.1"
                     /translation="VEGRKKLKALAGEGEVVPTFNDMIVKACALALKEFPRANGSYKD
                     GKFELYSRVNVGVAVAAQDALVVPTVFDADKKGLREISADTKRLAGKVRDGAITPPEL
                     SGGTFTVSNLGMFGVTNFSPVVNLPQAGILAVGALGPKPVVDGSGEVVVRQVMGVTLA
                     CDHRILYGADGAQFLARIRALLEEPISLAL"
BASE COUNT          479 a         1274 c         1113 g          492 t
ORIGIN      
        1 cctcgcgatc cgggaagcag cgccgcagga tctccgccgc ctcctcgtcg gtgcgctggt
       61 cgagcagcgg gaagacgacg cggctggtgc cgaggtagaa gttcgcgtag gaggcggcga
      121 gccggtcgcc accgcgccgc ggcttcgtcc ccggcaccgg ctcgacgccg gctgcctcct
      181 cggcggtggt cagcagcgga ccgggggagg ggaggaggac gacctcgaag gggcgcccgc
      241 gggcgtcggt cgcggcttcg agccgctcgc gcgcgtcgcg cgagatcgca tgctgggggt
      301 cccgctcggc gtcggtccaa gtcagcacca cgacacccgg cgcggcgaag caggcgaggt
      361 tgtcgacgtg cccgtcggtc tcgtcctcgt ggacgccctg gccgagccag accaccttct
      421 ctgcaccgag gtaggcgcag agcgccgcct cgatctgcgc ccgcgagagc tgcgggttgc
      481 gattgcggtt gagcaggcac tgttcggtgg tcagcacggt gccctcgccg tcgacgtgga
      541 tcgccccgcc ctccatcacc agcggcgccg ggtagcggtc ggcgccctcg acctccagca
      601 ccttggcggc gaccagctcg tcccggtccc aggattcgta gagcccgccg tggtgcccgc
      661 cccaggcgtt gaagcgccag tcgatcccgc gccggtggcc ggcgccgtcg atgacgaagg
      721 tcgggccggt gtcgcgcatc caggcgtcgt cgagggcgac ctcgaccacg cgcacggcgg
      781 ccggcagcag ttcgcgggcg cgctcgaact gggcctccga gaccgccatc gtgaccggct
      841 ccgacggtgc gatcgccgcc gccaccgcgg cgtaggcgcg ctgcgccggt tcggcgccga
      901 gccgccagtt gtcggcccgc tccggccagg ccatccagca gccgtcgtgg ggctcgaact
      961 ctcccggcat ccggaagccg tcggcggcgg gtgtgctgtc gagggtcgcg ctcaccgtca
     1021 ccgattgtcg ctcaccgccg caaccgccta cagtcgatct gtgcagttgc tcaccgccta
     1081 cgaggttctc gactcgccgg cccgcacccg accggccgat cgcgagccgt tccgggtcgg
     1141 cgccgtgcag gagcgctggc acgaggatcc ggccgagcat cgcgccgcgc tcgccgccgg
     1201 ggtcgagctg gccgccggcg agggcgctcg gctggtctgc ctgcaggagc tgaccttgag
     1261 cccttacttc gcggtcgacc cagccggcgc cgccgccgcc ggggttgagc ccgagtcgct
     1321 gccgggcggc ccgaccttcg agctcgccgc cgagctggcg cgtgcgcacg ggatccacgt
     1381 ccacgcctcg ctgttcgagc gcaccgagga tggggagggg gacgggcgcg gcttcaacac
     1441 cgcgatcctg gtcgcgcccg acggcgagct cgccgcccgc acccgcaagc tccacatccc
     1501 ggtcaccgcc ggctactacg aggatcgtta cttcctgccc gggcccgcca ccggcgatcc
     1561 ctacccggtg atcgccctcg accaggcccg gctcggcctg ccgacctgct gggaccagtg
     1621 gttcccggag ctggcccgct cctacagcct cgccggcgct gaggtgctcg tctacccgac
     1681 cgcgatcggc tccgagcccg accacccggg gttcgacacc cagccgctct ggcagcgggt
     1741 gatcgtcgcc aacgggatcg ccaacggcct cttcatggtc gccgtcaacc gcaccggctc
     1801 cgagccgccg ctcgccttct acggctcctc cttcatctcc gacccctacg gccgggtctt
     1861 ggtcgaagcc ccccgcgacg agcccgccgt cctggtcgcc gacctcgacc tcgaccagcg
     1921 ccgcgactgg ctcgagctct tccccttcct caccacccgc cgccccgacg cctacggctc
     1981 gctggtcgac cccggctgag tccgggcgct tccggcccct tcggggaagc gcggcagccc
     2041 gtcgtcgggg atcgggtacc acgcgggcgc cgaggagacc caggcccgcc agctcggctc
     2101 gacttcgggg tcgccttcga ccgcgcccag ccgcaccgcg atcagttcgc cgccctcggg
     2161 gtcgccgccg aacacgtgcc cgccgcagcg ccggcagaac gacttcggcc gcccgccgcc
     2221 atccggcacc cagttgccga cctcgccggc ccccgcggtc agctcgaacc cgctcgcatc
     2281 gaccaacccg ttgaaggtcc agggcacgcc gctgcggtgc tggcagcgtt cgcagtgaca
     2341 gtagccggcc gagagaaacg gctcggtgac ccggaaccga accgtcccgc aagcgcaacg
     2401 cccggtcagt ggctgctcgg cggaaggcat gggacagagg ctaatccgcc ctggcgaatt
     2461 cggcttggaa tgtgaggtcc atcactgtgt gagggcgctc acaaacgtgc gttcgtattc
     2521 gtccatactt gtatacaagc taaccaccag atggagagtc accccaaatg agtcacaaga
     2581 gctggacctt caccacgtac gagaacggca agccggtcgc taccgagagc aatctcgaca
     2641 gccgtcaggc cctcgccgcg atccgtgccg cgatggtcgg tgaaccggtc gccccggtcg
     2701 cccagcaccg ccgcgaggtc gcggagccgg tcgagctcgc tcacgccgcc tgaagagcgt
     2761 gagcgggttc gcccgcacta gcttctaaag agccaggctg atcggctctt ccagcagggc
     2821 cctgattcgc gccaggaact gcgcgccatc cgctccgtag aggatgcggt gatcgcaggc
     2881 cagggtcacg cccattacct ggcgcaccac cacctcgcct gagccatcca cgaccggctt
     2941 cggcccgagc gctcccacag ccaagatccc ggcctggggc aggttgacca cgggagagaa
     3001 gttcgtaacc ccgaacatcc ccaggttgga gaccgtgaag gtcccaccgg atagctcagg
     3061 cggggtaatc gctccgtccc ggactttccc ggcgagccgc ttggtgtccg cggagatctc
     3121 tcgcagaccc ttcttgtcgg cgtcgaagac ggtggggacg acaagcgcgt cctgagcggc
     3181 gaccgcgacc ccgacgttga cccgcgaata cagctcgaac ttgccgtcct tgtatgagcc
     3241 gttggcgcgc gggaactcct tcaacgccag cgcgcaggcc ttgacgatca tgtcgttgaa
     3301 ggtcggcacc acctcgccct cgccggccag cgctttcagc ttcttacggc cctcgacc
//
LOCUS       DASRIG010000024         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1063929, whole genome shotgun sequence.
ACCESSION   DASRIG010000024 DASRIG010000000
VERSION     DASRIG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063929"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFB52_00520"
     CDS             <1..531
                     /locus_tag="VFB52_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZO04846.1"
                     /translation="PDGSGEYVLAGTSAAGTPTGPGIDTFVTSLPVQAGDLIGLENLS
                     TAAKIGVAFPAGSEYHVWFGSFSEGASTATASSDFAYANQEIGFNADVEFLAPPVGPP
                     PPVTPPAQCVVPKLKGKKLKAAKKKINAAGCKLGKVTKRKGATAANGKVAGQRPKAGK
                     TVAAGTKVKVTLAAPS"
     gene            562..1713
                     /locus_tag="VFB52_00525"
     CDS             562..1713
                     /locus_tag="VFB52_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04847.1"
                     /translation="MSSKLATLLIAAAIVALSASPAVAASEFAQPGAIEGTVTASPGK
                     TPVEGVEVCASASVEGEWFTQCEESKGNGHYEVIGLPEALYRVQFKSGEGGKDLATQY
                     YRNTTNPNAAKLIQIDERETVTNIDAEMQPKASIFSASWGTDNAVGCPSGAQGLDNIP
                     VTFSWFIRRSSIQTADFRIVRSDGSVAKPTCALQFPPNERDEAQTVNLIGDFGDSVSG
                     PTPKAIRVVGTLEGKPPGADGWGQIGRLPKIGVDPISDGPYIVDAWTLTPSIYRGDQN
                     RCATGKAFLRVMWSNGLTAYPTGEEVGAAVSASYRALYKLPNGKTVAVAPLAVADLHD
                     HESSFNADNMHDLCLPKLPRRARLTGVTIAADLIQDPNGDPNLAQKFKP"
     gene            1761..>2802
                     /locus_tag="VFB52_00530"
     CDS             1761..>2802
                     /locus_tag="VFB52_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO04848.1"
                     /translation="MSADYDYFKTLVRSYHPVIAIDTVEEDRVEHLLLRTATELGVPL
                     MEWSITSGMRRHGESAGPMYGTTDPSRALAHIEESSNNAIYWLKDFTPHLSAHGVARQ
                     LRDLTTRMVESHATTTVVLTGAGLELPDDAVPAATYFELPTPSKDEYAQTIDWVIQSV
                     AWRDNAQIDTSLLDKPALIRALSGLTLAQARQAIAYAALDDGRLSPEDVPGIAGMKAK
                     MINEGGILEYFPAEERHRELGGFAGLKAWLEDARMGFSEQAEAFNLSPPKGVLIVGVQ
                     GCGKSLAAKVIARQWGLPLLKLDAGRLYGSLMGESEKNFRRATFLAEALAPDVLWIDE
                     IEKGFAPNRGDSD"
BASE COUNT          511 a         1018 c          909 g          364 t
ORIGIN      
        1 cctgacggca gcggcgagta cgtgctcgcc gggacgagcg cggccgggac gccgaccggt
       61 cccggcatcg acaccttcgt gaccagcctc ccggtccagg ccggcgacct gatcgggctg
      121 gaaaacctct cgaccgcggc aaaaatcggc gtcgccttcc cagccggctc cgaataccac
      181 gtttggttcg gcagcttctc cgaaggcgcc agcaccgcga cggcctcctc cgacttcgcc
      241 tacgccaacc aggagatcgg cttcaacgcc gacgtcgagt tcctcgcccc accggtcggc
      301 ccgcccccgc cggtcacccc gccggctcag tgcgtcgtgc cgaagctcaa gggcaagaag
      361 ctgaaagcgg cgaagaagaa gatcaacgcc gccggctgca agctcggcaa agtgaccaag
      421 cgcaaaggcg cgacggcggc caacggcaag gtcgccggcc agaggccgaa agcgggcaag
      481 accgtcgccg ccgggaccaa agtgaaggtg acgctggccg cgccgagtta gacctcgacc
      541 gggggatcta tcctcccggc ggtgtcgtcc aagctcgcaa ctctgctgat cgccgctgcg
      601 atcgtcgccc tgtccgcgtc cccggcggtc gccgcgtcgg aattcgccca gccgggcgcg
      661 atcgaaggga cggtcaccgc gtcgcccggc aagacccccg tcgaaggggt cgaagtctgc
      721 gccagtgctt cggtggaagg cgaatggttc acccagtgcg aggaatcgaa ggggaacggc
      781 cattacgaag tcatcggcct gcctgaagcc ctgtaccggg tccagttcaa gagcggcgaa
      841 ggcggcaagg acctcgccac ccagtactac cggaacacga ccaacccgaa cgcggcgaag
      901 ctgatccaga tcgacgaacg cgagacggtc accaacatcg acgccgaaat gcagccgaag
      961 gcctcgatct tcagcgcctc gtggggcacc gacaacgcgg tcggctgtcc cagcggggcg
     1021 caggggctcg acaacatccc ggtcaccttc agctggttca tccgccgcag ctcgatccag
     1081 accgccgact tccggatcgt gcgcagcgac ggcagcgtcg cgaagccgac ttgcgccctc
     1141 cagttcccgc cgaacgagcg cgacgaggcc cagacggtga acctgatcgg cgacttcggc
     1201 gactcggtca gcggcccgac gccgaaggcg atccgcgtgg tcggcacgct ggagggcaag
     1261 cccccgggcg ccgacgggtg gggccagatc ggccgcctgc cgaagatcgg cgtcgacccg
     1321 atctcggacg gcccctacat agtcgacgcc tggacgctaa ccccctcgat ctaccgcggc
     1381 gaccaaaacc gctgcgcgac cggcaaggcc ttcctgcggg tgatgtggtc caacggcctc
     1441 accgcctacc cgaccggcga agaggtcggc gccgcggtct ccgcctccta ccgcgccctc
     1501 tacaagcttc cgaacggcaa gaccgtcgcc gtggcgccgc tcgcagtcgc cgacctccac
     1561 gaccacgaaa gctcgttcaa cgccgacaac atgcacgacc tctgcctgcc gaagctcccg
     1621 cgcagggccc ggctcaccgg cgtgacgatc gccgccgacc tgatccagga ccccaacggc
     1681 gacccgaacc tcgcccagaa gttcaagccc tgaagaccac cggtttaagg gcctccgggc
     1741 gttttgtcga aacacgggca atgagcgccg actacgacta cttcaagacg ctggttcgct
     1801 cctaccaccc ggtgatcgcg atcgacaccg tggaggagga ccgggtcgag catctgctgc
     1861 tgcgcacggc gaccgagctg ggcgtgccgc tgatggagtg gtcgatcacc tcgggaatgc
     1921 gccgccacgg cgagtcggcg gggccgatgt acgggaccac cgatcccagc cgggcgctcg
     1981 cccacatcga ggaatcgagc aacaacgcga tctactggct gaaggacttc accccccacc
     2041 tcagcgccca cggcgtcgcc cgccagctgc gcgacctgac cacgcgcatg gtcgagtcgc
     2101 acgcgacgac gaccgtggtg ctgaccggcg ccgggctgga gctgcccgac gacgccgtcc
     2161 cggccgcgac ctacttcgag ctgccgaccc cgagcaagga cgaatacgcg cagacgatcg
     2221 actgggtgat ccaatcggtc gcctggcgtg acaacgccca gatcgacacc tcgctgctcg
     2281 acaaaccggc gctgatccgc gcgctcagcg gcttgaccct ggcccaggcg cggcaggcga
     2341 tcgcctacgc ggcgctcgac gacggccggc tcagccccga ggacgtgccc gggatcgccg
     2401 ggatgaaggc gaagatgatc aacgagggcg gcatcctcga gtacttcccg gccgaagagc
     2461 gccaccgcga gctgggcggc ttcgccgggc tgaaggcgtg gctcgaagac gcccgcatgg
     2521 gcttctccga gcaggccgag gcgttcaacc tctcgccgcc gaagggggtg ctgatcgtcg
     2581 gcgtgcaggg ctgcggcaag tcgctggcgg cgaaggtgat cgcccgccag tggggcctgc
     2641 cgctgctcaa gctcgacgcc ggccgcctct acggcagcct gatgggcgag tcggagaaga
     2701 acttccgccg cgccaccttc ctcgccgagg cgctggcccc cgacgtcctc tggatcgacg
     2761 agatcgagaa gggcttcgcg cccaaccgcg gcgacagcga cg
//
LOCUS       DASRIG010000025         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_536510, whole genome shotgun sequence.
ACCESSION   DASRIG010000025 DASRIG010000000
VERSION     DASRIG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536510"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFB52_00535"
     CDS             complement(<1..650)
                     /locus_tag="VFB52_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04849.1"
                     /translation="MLFPTVQFAIFFPIVLALSWLLMPKQKLWKPYILLASYVFYAAA
                     DIRFCLLLGGITVANQIAAKAIHASEDERRRKWICAAAVVFDLGVLGVFKYYGFFTQN
                     VGDLLDSFGLGMPLPLLSIALPVGVSFFTFQAISYTVDVKRREFEPASLMDVGIYLSF
                     FPHLVAGPIVRAREFIPQLATARDPNHVAVGAGLGLVGLGLIKKVMIADYLGREIV"
     gene            727..1419
                     /locus_tag="VFB52_00540"
     CDS             727..1419
                     /locus_tag="VFB52_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04850.1"
                     /translation="MSRRLHTLAFATALIATMALLWTSASAALACGGTETAKPRKTVN
                     PDGHAPLAIGDSTMLLALPDLARVGYQVNARGCRTLDEGLDVMRDYKRRNALPHLVVL
                     FLGADASVSMQQLQKAFHIAGPKRVLGLVTPIELGGGTSSDADHVRSAVKRHPRRSVL
                     LDWVAFSNGHPEWFQPDNLHLTFDGAAGLARLLKQALPYAEAGKFPGEPEKPTGKAGL
                     SPAKPAPDKEPK"
     gene            1458..1964
                     /locus_tag="VFB52_00545"
     CDS             1458..1964
                     /locus_tag="VFB52_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04851.1"
                     /translation="MLAKTLRHLRSNAIAYAALFVALSGTAYAATQLPKNSVGAKQLK
                     KQSVNAAKVKKNSLTGNQINEAKLGKVPNAAKLDGKSPGDFLAAGGTAVNADKLDGVD
                     SAIFPPVTAPFLIFAWVTAFFFSFAVVTAFFFSCLAPTLFLPSCVAAKAVPPARSRNT
                     NSVEATFA"
BASE COUNT          368 a          766 c          697 g          319 t
ORIGIN      
        1 tcgacgattt cccgccccaa gtagtcggcg atcatcacct tcttgatcag gccgaggccg
       61 accaggccga gcccggcgcc gaccgcgacg tggttggggt cgcgcgcggt cgccagctgg
      121 gggatgaact cgcgggcgcg cacgatcggg ccggcgacca ggtgcgggaa gaaggaaagg
      181 tagatcccga cgtccatcag cgaggctggc tcgaactcgc gccgcttcac gtcgacggtg
      241 taggagatcg cctggaaggt gaagaagctg accccgaccg ggagcgcgat cgagagcagc
      301 ggcagcggca tcccgagccc gaacgagtcg agcagatcgc cgacgttctg ggtgaagaag
      361 ccgtagtact tgaagacgcc gagcacgccg aggtcgaaca cgaccgcggc ggcgcagatc
      421 cacttgcgcc ggcgctcgtc ctcgctcgcg tggatcgctt tcgcggcgat ctggttggcg
      481 accgtgatcc cgccgagcag caggcagaag cggatgtcgg cggccgcgta gaagacgtag
      541 ctggcgagca ggatgtacgg cttccagagc ttctgctttg gcatcagcag ccagctcaga
      601 gccagcacga tcgggaagaa gatcgcgaac tggacggtgg ggaatagcaa tggctgctcg
      661 tgcctctgtt aggttgccgg cggcctccgc ccgctgcgag cgggcgatgc tattcgatcc
      721 ctttcgatga gccgccgact ccacacgctc gccttcgcca cggctctgat cgccacgatg
      781 gcgctgctct ggacaagcgc ctccgcggcc ctcgcctgcg gcggcaccga gaccgcgaag
      841 ccgcgcaaaa ccgtcaaccc agacgggcac gcgccgctgg cgatcggcga ctcgacgatg
      901 ctgctggcgc tgcccgacct cgcccgggtc ggctaccagg tgaacgcgcg cggctgccgc
      961 acgctcgacg agggcctcga cgtgatgcgc gactacaagc gccgcaacgc cctcccccac
     1021 ctggtcgtgc tcttcctcgg cgccgacgcc agcgtctcga tgcagcagct gcagaaggcc
     1081 ttccacatcg ccgggcccaa acgggtgctc gggctggtca ccccgatcga gctcggcggc
     1141 ggcacctcga gcgacgccga ccacgtgcgc tcggcggtca aacgccaccc gcgccgctcg
     1201 gtcctgctcg actgggtcgc cttcagcaac ggccaccccg agtggttcca gcccgacaac
     1261 ctccacctca ccttcgacgg cgccgccggc ctcgcccgcc tgctcaaaca ggcgctgccc
     1321 tacgccgagg ccggcaaatt ccccggggaa ccggagaaac cgaccgggaa agcgggtctc
     1381 agcccggcca agccggctcc ggacaaagag ccgaagtagc ttgttcgccg ccgccggggc
     1441 tgcataggat tcgcgccatg ctcgccaaga cgcttcgcca cctccgctcg aacgccatcg
     1501 cctacgcggc ccttttcgtc gccctctcgg gcaccgccta cgcggccacc cagctgccca
     1561 agaacagcgt cggcgccaag cagctgaaga agcagtcggt gaacgcggcg aaagtcaaga
     1621 agaactccct gaccgggaac cagatcaacg aggccaagct cggcaaggtc ccgaacgcgg
     1681 ccaagctcga cggcaagtcg cccggcgact tcctcgccgc cggcggcacc gcggtcaacg
     1741 cggacaagct cgacggcgtc gactcggcga tcttcccacc ggtcaccgcg cccttcttga
     1801 tcttcgcctg ggtcacggcg ttcttcttca gtttcgccgt ggtcaccgcg ttcttcttca
     1861 gctgcttggc gccgacgctg ttcttgccga gctgcgtagc ggcaaaggcg gtgccgccgg
     1921 cgaggagcag gaacaccaac agcgtcgagg ccacgttcgc gtaggtgagt cgtttccgga
     1981 tcacgatcgt ctccttgatc gacctgggcc tctagcggac accgacgacg tcgacggcgc
     2041 cgttgctggc gctcaccccc gtgccggcag tgcactccgc gtcgaccagg aggttgaccg
     2101 tccgcgtggt cggggtgccc ggctcgaacc tgctgccaag gtagggcccg
//
LOCUS       DASRIG010000026         6708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_13950, whole genome shotgun sequence.
ACCESSION   DASRIG010000026 DASRIG010000000
VERSION     DASRIG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13950"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFB52_00550"
     CDS             <1..1104
                     /locus_tag="VFB52_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZO04852.1"
                     /translation="ECAEAGVTFIGPSAEAMKRSGDKATARALAKELDVPINEGSDVI
                     EDEEEARAIAGEVGYPVLLKASAGGGGRGMRLVESEDELAGAYAAAHGEAQAAFGDGR
                     LFVERYVRHARHVEVQVLGDGEGGAIHLGHRDCTLQRRYQKLVEEAPAYGLSPELDEQ
                     ITSAAVRLISALSYSGAATCEFLVDPGRGSAGFLEINARLQVEHPISEVVTGVDVVRE
                     MLRIAGGEGLSVSQDDVTISGHAIEVRINAESPERDFAPTPGTLTEWAAPVATDVRVD
                     TACFPGWSVPPYYDSLLAKVIASGPDREAALARMRQALTHLRVSGVETTAGFALDVLS
                     HPDVVAGRVHTKWVEETFMPEWNARNAGEEEAA"
     gene            1104..2573
                     /locus_tag="VFB52_00555"
     CDS             1104..2573
                     /locus_tag="VFB52_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04853.1"
                     /translation="MATIEIVDQTLRDGQQSYWGMRMQAGQLLPVADAIDSAGYRIVD
                     LTGSSIFEVMVRFRQENPWKGLDAIRAALPRSVLRAGTRTNGIVGMNVTPDSIVELWI
                     RTLAKHGIESLWIFDCLHDAENMIRVAKIARDAGLKPSPQLNFSQSPVHTDDYYADVI
                     DRMIAGGAAETILLGDEAGVLGPERARRWIRQSQERASAGGVPLEMHFHNRTAMANLN
                     HIIGVEEGVTIVHSAISTLANGVSMPSTEVTVDNMRRLGHEVPLDDSRLAEVAEHFGA
                     LADEEGFQRGAPVEYQVANIQQQFPGGMMGTLREQLKQVGMTDKLPAVLEEAIRVREE
                     LGWPIMATPFSQLVGIQALLNVVQGERYATIPDENLMYLAGWYGKPPGEVDQEVLDKA
                     AATARGKEMIDAGHAPQPSLKEIRAEYGENLSDEELLLRYLIAGTDVDDMYAADNPIE
                     PIYPIGGAAGLGWIKDLLATSGSRGVKASRGPVSVTLRR"
     gene            2585..3037
                     /locus_tag="VFB52_00560"
     CDS             2585..3037
                     /locus_tag="VFB52_00560"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZO04854.1"
                     /translation="MELAYEFTYDAVLAPKVPVGEGPFGKRHVREVTGGKVTGERING
                     KVGSGGGDWILVGPDGWGRLDVRLQIHTDDGADIYVQYFGIVEYNDAAVGANEGKGSS
                     DYPDHYFRTAPRLETGDPRYEWVNRSLFVAEGRLHPGPVVEYRVYRVL"
     gene            3225..4553
                     /locus_tag="VFB52_00565"
     CDS             3225..4553
                     /locus_tag="VFB52_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO04855.1"
                     /translation="MKHQNRRGATSSKWALLMALLALGVCAFALTACGGDDDSSSSES
                     TSAETTEGGGGGGEEAKGEPIVTWTYTDVNTEGPQYKNIEETARVYEEWINANGGIGG
                     RPLEANFCDARGTPTGVAACGREAVAGGAVAVVGNFTFTGDAIIPILEKGKTAQWGNC
                     CAIAPSELVSPISFPMGNQPLYSVGLVKKAVEDGCKKINGVIIEGAEAFIPLMENAAK
                     AEGTSIGKFVTLPATAQDYAPQVAEATSGDADCLVMIVSETPYIAWMPAFAQSGSEAR
                     MYGPQGNFNEKAVEGFGEVVEGDVNAGMYPDISTEAWADYRAALEEYDADTGQDYNSL
                     GGMGTWAAYEGFKQVVESMGSEELNNETFLKAAETAVIDLPGMVPPEDFALNWGEDGG
                     PEGYDRLVNRCVVFSEFDASGKLIPLGNEFTDVSEIAGGTKPMDCGPPFG"
     gene            4677..>6708
                     /locus_tag="VFB52_00570"
     CDS             4677..>6708
                     /locus_tag="VFB52_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04856.1"
                     /translation="MEEVIRFALLGLGIGATYALASQGLIIIYRGSGVLNFALGAIGM
                     AGAYAWWELTANQGWSFFPALIAGVLFSAAIGAAVHLLIMRPLRHSAPLVRVIATLGV
                     LITIQAIAVLRYTATSKFVLSDLPTDNVHIHNTIEISADRLILLGIAVALTVGLWLFY
                     RYTKFGLATAAVSESERSASALGLSPDRVATLNWALGCGLAGLAAILIVPIVTLQPAV
                     LTNLVLAATAAALVAGFRSFPIALIAGLVIGIAQTEVDRYVDQAGVGKAVPFVLIVLW
                     LVIRGQALPLRDYLLQKLPTIGTGQIHRVGLAIGAIVGVVLLSIMSPIWIDAITVTIC
                     VAVILLSIVVLTGYTGQLSLAQYAFAGFGAWVGGRLLATTDIPFLLGCIAGIAATVPL
                     GVLFGLPAVRTRGINLAIVTLGLGSAIELVLFSNTDFTGGFGGTQIGDPSLFGWDINA
                     ASHPTRYGLIALACLLVATLVVGNIRRGRTGRRLIAVRTNERAAAALGISVPGAKLYA
                     FAVGAAIAGLGGLLIAFRTPSISFVQFDSFTSITMVAYAMIGGLGYLFGPVIGATLAP
                     GAFSERLLNEIDSGIGKYIPLIGGVSLIVLLLVNQNGIVKEQIAQVAFLRSKLAGFLP
                     FLAVKEQPKRELPKASATHEKKVSAKSLQVENLTVKYGGTVAVDDVSLTVKPG"
BASE COUNT         1117 a         2320 c         2225 g         1046 t
ORIGIN      
        1 gagtgcgcgg aggccggggt cactttcatt gggccgtccg ccgaggcgat gaagcgcagc
       61 ggggacaagg cgacggcgcg ggcgctggcg aaagaacttg acgtgccgat caacgagggg
      121 tcggacgtga tcgaggacga ggaggaagct cgggcgatcg cgggggaggt cggctatccc
      181 gtcctgctca aggcctcggc cggcggcggc gggcgcggca tgcggttggt cgagtccgag
      241 gacgagctgg ccggcgccta cgcggccgcc cacggcgagg cccaggccgc cttcggcgac
      301 gggcgcctct tcgtcgagcg ctacgtgcgc cacgcccgcc acgtcgaggt ccaggtcctc
      361 ggcgacggcg agggcggcgc gatccacctc ggccaccgcg actgcaccct gcagcgccgc
      421 taccagaagc tggtcgagga ggcgccggcc tacgggctct cgcccgagct cgacgagcag
      481 atcaccagcg ccgccgtgcg gctgatcagc gccttgagct actccggcgc cgccacctgc
      541 gagttcctcg tcgaccccgg ccgcggcagc gccggcttcc tcgagatcaa cgcccgcctg
      601 caggtcgagc acccgatctc cgaggtcgtc accggcgtcg acgtggtccg cgagatgctg
      661 cggatcgccg gcggcgaggg cctctcggtc agccaggacg acgtgacgat ctccggccac
      721 gcgatcgagg tgcggatcaa cgccgagtcg cccgagcgcg acttcgcccc gacccccggc
      781 acgctgaccg agtgggcggc cccggtcgcc accgacgtgc gcgtcgacac cgcctgcttc
      841 cccggctgga gcgtcccgcc ctactacgac tcgctgctcg ccaaggtgat cgccagcggg
      901 cccgaccgcg aagcggcgct ggcgcggatg cgccaggcgc tcacccacct gcgggtgagc
      961 ggggtggaga cgaccgcggg cttcgcgctc gacgtgctct cgcaccccga cgtggtcgcc
     1021 ggccgcgtcc acaccaagtg ggtcgaggag actttcatgc ccgagtggaa cgcccgcaac
     1081 gcgggcgagg aggaggcggc ctgatggcca cgatcgagat cgtcgaccag accctgcgcg
     1141 acggccagca gagctactgg gggatgcgca tgcaggccgg ccagctgctg ccggtggccg
     1201 acgcgatcga ctcggccggc taccgcatcg tcgacctcac cggctcctcg atcttcgagg
     1261 tgatggtccg cttccgccag gagaacccgt ggaaggggct ggacgcgatc cgcgccgcgc
     1321 tgccgcgctc ggtcctgcgc gccggcaccc gcaccaacgg gatcgtcggg atgaacgtca
     1381 ccccggactc gatcgtcgag ctctggatcc gcacgctcgc caagcacggg atcgaaagcc
     1441 tctggatctt cgactgcctg cacgacgccg agaacatgat ccgggtcgcg aagatcgccc
     1501 gcgatgcggg cctgaagccc tcgccgcagc tcaacttctc ccagtccccg gtccacaccg
     1561 acgactatta cgccgacgtg atcgaccgga tgatcgccgg cggcgccgcc gagacgatcc
     1621 tgctcggcga cgaggccggc gtgctcggcc ccgagcgggc gcggcgctgg atccgccaaa
     1681 gccaggaacg cgcgtcggcg ggcggggtgc cgctggagat gcacttccac aaccgcaccg
     1741 cgatggccaa cctcaaccac atcatcggcg tcgaggaggg ggtgacgatc gtgcactcgg
     1801 cgatctcgac cctcgccaac ggggtctcga tgccgtcgac cgaggtcacc gtcgacaaca
     1861 tgcgccggct cggccacgaa gtgccgctcg acgactcccg cctggccgag gtcgccgagc
     1921 acttcggcgc gctcgccgac gaggaaggct tccagcgcgg cgccccggtc gagtaccagg
     1981 tcgcgaacat ccagcagcag ttccccggcg ggatgatggg cacgctgcgc gaacagctga
     2041 agcaggtcgg gatgaccgac aagctgccgg cggtgctcga ggaggcgatc cgggtgcgcg
     2101 aggagctggg ctggccgatc atggcgacgc cgttctcgca gctggtcggc atccaggcgc
     2161 tgctgaacgt ggtccagggc gagcgctacg cgacgatccc cgacgagaac ctgatgtacc
     2221 tggccgggtg gtacggcaag ccgccgggcg aggtcgacca ggaggtgctc gacaaggcgg
     2281 cggcgacggc gcgcggcaag gaaatgatcg acgccggcca cgccccgcag ccgagcctga
     2341 aggagatccg cgccgagtac ggcgagaacc tctccgacga ggagctgctg ctgcgctacc
     2401 tgatcgccgg caccgacgtc gacgacatgt acgcggccga caacccgatc gagccgatct
     2461 acccgatcgg cggcgccgcc gggctcggct ggatcaaaga cctgctcgcc acctcgggct
     2521 cgcgtggggt gaaggcgagc cgtggcccgg tcagcgtcac cctgaggcga taggagccgc
     2581 cgcgatggag cttgcctacg agttcaccta cgacgccgtc ctcgccccca aagtgccggt
     2641 cggcgagggg ccgttcggca agcgccacgt gcgcgaggtg accggcggca aagtcaccgg
     2701 tgagcggatc aacggcaagg ttggttcggg cggcggcgac tggatcctgg tcggaccaga
     2761 cgggtggggc cggctcgacg tccgcctgca gatccacacc gatgacggcg ccgacatcta
     2821 cgtgcagtac ttcggcatcg tcgagtacaa cgacgccgcg gtcggggcca acgaaggcaa
     2881 gggctccagc gactaccccg accactactt ccgcaccgcc ccccgcctgg agaccggcga
     2941 cccccgttac gagtgggtca accgctcgct gttcgtcgcc gagggtcgtc tgcaccccgg
     3001 cccggtggtc gagtaccgcg tctaccgcgt cctctagccc gggtccgaca aagcggcgaa
     3061 gcccgcctct caagcggctc ccaggggtcg taggaacacc tccttcctgg ctctagaaag
     3121 ccgcttggtg aatctccaac gctgtcagac catcgtgtcc cgcggcccaa tcgacgctct
     3181 tacgcttgcc ccaagtccgg ttttgctatc gaggaggaga gtcgatgaag catcagaacc
     3241 gtcgcggtgc gacgagttcg aaatgggccc tactgatggc cctgctcgcg ctcggcgtct
     3301 gcgcatttgc gctgaccgcg tgtgggggag atgacgacag ttcgtcaagc gaaagcacgt
     3361 cagctgaaac caccgaaggc ggtggcggcg gcggtgaaga agccaaaggc gaaccgatcg
     3421 tcacctggac ctacaccgac gtgaacacgg aaggtccgca gtacaagaac atcgaagaaa
     3481 ccgcgcgcgt ttacgaggaa tggatcaacg cgaacggcgg catcggcggt cgtccgctcg
     3541 aagccaattt ctgcgatgcc cggggcacgc cgactggcgt ggccgcgtgt ggtcgtgaag
     3601 cggtcgccgg cggtgctgtc gccgtggtcg gcaacttcac cttcaccggt gacgcgatca
     3661 tcccgattct cgaaaaaggc aaaaccgccc agtggggtaa ctgctgcgcg atcgcgcctt
     3721 ctgaattggt gtctccgatc agcttcccga tggggaacca gccgctctac tcggttggcc
     3781 tcgtcaaaaa agctgtcgaa gacggctgca aaaagatcaa cggcgtgatc atcgaaggcg
     3841 ctgaagcctt catcccgctg atggaaaacg cggccaaagc cgaaggcacg agcatcggca
     3901 aattcgtaac cctgccggcc accgcgcagg actacgcgcc tcaggttgcg gaagcgacct
     3961 ccggcgatgc cgactgcctg gtgatgatcg tctccgagac gccatacatc gcctggatgc
     4021 ccgcgttcgc gcagtccggc tctgaagccc gcatgtacgg gccgcagggc aacttcaacg
     4081 aaaaggccgt cgaaggattc ggcgaagtcg ttgagggtga cgtgaacgcc ggcatgtacc
     4141 cggacatctc cacggaagcg tgggctgact accgggccgc gctggaggaa tacgacgccg
     4201 ataccggcca ggactacaac agtctcggcg ggatgggcac gtgggccgct tacgaagggt
     4261 tcaaacaggt tgtcgaatcg atggggagtg aagaactcaa caacgaaacg ttcctgaaag
     4321 cagccgaaac cgcagtcatc gatctcccgg gcatggttcc gcccgaggac ttcgccctca
     4381 actggggcga ggacggtggc ccggaaggct acgaccgcct ggtcaatcgc tgcgtcgtct
     4441 tcagcgaatt cgacgcgagc ggcaaattga tcccgctcgg taacgaattc accgacgttt
     4501 ccgagatcgc gggtggcacg aaaccgatgg attgcggtcc gcccttcggc tgatccgcaa
     4561 ctggctcacc gaccccgggc gctgtgcccg gggtcggtgg cccgcttcgc agaactgatt
     4621 cttggcagca ctgattcacc accaactcag caccctagga ccgaaagaga gcactcgtgg
     4681 aggaagtaat ccgatttgcg ctgttgggcc tcggcatcgg cgccacgtac gcgctcgcct
     4741 cgcagggtct gatcatcatt taccgcgggt ccggggtgct gaacttcgcc ctcggtgcga
     4801 tcggtatggc cggcgcctac gcctggtggg agctgaccgc caaccagggt tggtccttct
     4861 tcccggccct gatcgccggc gtgctcttct cggccgcgat cggcgccgcg gtccacctct
     4921 tgatcatgcg gccgctcaga cattcagcgc cgctggtccg cgtgatcgcc accttgggcg
     4981 tcctgatcac gatccaggcg atcgccgtgt tgcgctacac ggcgacctcc aagttcgtcc
     5041 tctccgatct tccgaccgac aacgtccaca tccacaacac gatcgagatc tccgccgacc
     5101 gcttgatcct gctcggaatc gccgtcgctc tcaccgtcgg cctctggctc ttctaccgct
     5161 acacgaaatt cggcctcgcg accgccgcgg tatccgagtc cgagcgctcc gcttccgcgc
     5221 tcggcctctc gcccgaccgc gtcgcgacgc tcaactgggc gctcggctgc ggcctcgccg
     5281 gcctcgccgc gatcctgatc gtcccgatcg tcaccctgca gccggcggtg ctgaccaacc
     5341 tcgtgctcgc cgccaccgcg gccgcgctgg tcgccggctt ccgctcgttc ccgatcgccc
     5401 tgatcgccgg cctggtgatc ggcatcgccc agaccgaggt ggacaggtac gtcgaccagg
     5461 ccggggtggg gaaggcggtc ccgttcgtcc tgatcgtgct ctggctggtg atccgcggcc
     5521 aggcgctgcc actgcgcgac tacctgctgc agaagctgcc gacgatcggc accggccaga
     5581 tacacagggt cgggctggcg atcggcgcga tcgtcggcgt cgtcctgctc tcgatcatgt
     5641 cgccgatctg gatcgacgcg atcacggtga cgatctgcgt cgcggtgatc ctgctctcga
     5701 tcgtcgtcct gaccggctac accgggcagt tatcgctggc gcagtacgcc ttcgccggct
     5761 tcggcgcctg ggtcggcggg cgattgctgg cgacgaccga catccccttc ctgctcggct
     5821 gcatcgccgg gatcgcggcg acggtcccgc tcggcgtgct cttcgggctg ccggcggtgc
     5881 gcacccgcgg gatcaacctg gcgatcgtca cgctcgggtt aggcagcgcg atcgagttgg
     5941 tcctgttctc gaacaccgac ttcaccggcg gcttcggcgg cacccagatc ggcgacccga
     6001 gcctgttcgg ctgggacatc aacgccgctt cgcacccgac ccgctacggc ctgatcgcgc
     6061 tcgcctgcct cctggtcgcg acgctcgtgg tcggcaacat caggcgcggg cgcacgggga
     6121 ggaggttgat cgcggtacga accaacgaga gagccgcggc cgcgctcggg atctcggtcc
     6181 cgggggcgaa gctctacgcc ttcgcggtcg gcgccgcgat cgccggcctt ggcggtctgc
     6241 tgatcgcctt ccgcaccccg tcgatcagct tcgtccagtt cgactccttc acctcgatca
     6301 cgatggtcgc ctacgcgatg atcggcggcc tcggctacct gttcgggccg gtgatcggcg
     6361 ccaccctggc gcccggcgcc ttctccgaac ggctgctcaa cgagatcgac agcgggatcg
     6421 gcaagtacat cccgctgatc ggcggcgtct ccctgatcgt cctgctgctg gtcaaccaga
     6481 acgggatcgt caaggagcag atcgcccagg tcgccttcct gcgctcgaag ctggccgggt
     6541 tcctgccctt cctcgcggtc aaggagcagc cgaagcgcga gctgccgaag gcgagcgcga
     6601 cgcacgagaa aaaggtcagc gcgaaatccc tccaggtcga gaacctgacc gtcaaatacg
     6661 gtggcacggt cgccgtcgac gacgtctcgc tgaccgtcaa gccgggcc
//
LOCUS       DASRIG010000027        11989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3666853, whole genome shotgun sequence.
ACCESSION   DASRIG010000027 DASRIG010000000
VERSION     DASRIG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666853"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFB52_00575"
     CDS             complement(<1..277)
                     /locus_tag="VFB52_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZO04857.1"
                     /translation="MRRLALGALVALLALSPTAGAATTQTSLSEIEGEVMCPVCGTLL
                     ELAESPQALRQKVFIKRLVAEGESKEQIKDALVAEYGEEVLALPEGDG"
     gene            complement(274..759)
                     /locus_tag="VFB52_00580"
     CDS             complement(274..759)
                     /locus_tag="VFB52_00580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZO04858.1"
                     /translation="MLAIVGLLVFGLLSKGSNLEPGDPAPTTALPRLEGEGTESLADY
                     RGSWVLVNIWASWCIPCREEAPALEALQGEYGGEKFTVLGIDSSDLSDDGRRFVEEFG
                     LTYPQLHDGDGDFADEFGATGVPESYLVDPRGKVRVLARGAVSEEYLENEVEPLLKGK
                     S"
     gene            892..964
                     /locus_tag="VFB52_00585"
     tRNA            892..964
                     /locus_tag="VFB52_00585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Gly,seq:ccc)
     gene            complement(1689..2066)
                     /locus_tag="VFB52_00590"
     CDS             complement(1689..2066)
                     /locus_tag="VFB52_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO04859.1"
                     /translation="MSLTNGHSANGNGALRAVENGSASLPVDWEHLARSTAHPLRVSI
                     LEILGMDGGRTLSPSELSQELQIPLSNTNYHVTELAKSGLIDMVKQRQVRGATEHFYC
                     LPGVAAKTEANPNAQLQPQAVAA"
     gene            2178..5042
                     /locus_tag="VFB52_00595"
     CDS             2178..5042
                     /locus_tag="VFB52_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04860.1"
                     /translation="MLVAFAAAFMAPSTVAASTGDVIAPQHPPAYTADDGWQAGTCNE
                     EPPESTEVCSVETPDQFFEGAAAHPNWGFTQFIVKHEAPGETPVAELKRVLVDLPVGL
                     SVNPGATPRCPLATFEAGAGGCPPASQVGESMVTASMPLLGTPIAPIPGVTEVAVYNL
                     VPEDGEPARFGLELAGNEVFLEADVEWEGDYHEYFEIEVPKALPLEIPLLVQALILKN
                     RLVFDGRSGDGTFITTPSTCLGEAGEGPGKSGSIYSTWLRAGSYDEFEEPGYVFPTSA
                     EPAFESPIPPGTSPKECDSIPYDPTIAVEPGTAETNSPAAASVEIEVPHLLPDYGEGE
                     NEQDSSVTKASTVTLPLGMGINPSAANGLETCTDAQFGKGTRNPIACPPASKVGTVTV
                     KSAPLPEGDLEGSVYVGEQLSRDPLSGEEYRIFIAAESARYGISVRLVGKVKADPVTG
                     QLSTTISEVPQVPFTSFDLDFNGGAHAVLSSPPTCGPNKTSTEMVPWAPIAAAAPGDE
                     FELTATPGGGECAKTMAARAFAPAFAVAPKANTAGAFSPLSMRIARSDGQQELKGVDV
                     TLPPGMTGKLAGISYCSEQAIAAAAANSGKAEKAASTCPAASLVGSATIDAGTGTDPL
                     RIGGKVFLSGPYKGAPLSLAVVTPATAGPFDLGTAVVRVALFLDPETAQIHAVSDPVP
                     DVYGGTQLSVRSVDVNLDRKDFTLNPTSCEPLAGGGALRGGGANPADPALFSAFPVAI
                     AFQTSNCEALKFRPKLTTRLFGGKKSTKRGQEPKFRAVLTARGGDANIGRAALTLSRA
                     LILEQGHIRTICRNAELAAGACPQRSIYGYAMAESPLLDEPLSGPVYLVPTTSDSGLP
                     DLLADLRGQVNIRLRGQIGAAKARLKTVFPMVPDVPVSKFVITMKGGKRGLLTNTRDL
                     CLKPVYSFLNFKAQNGKKLKKKKLPLRVPGCKGAKKRAKG"
     gene            5106..7805
                     /locus_tag="VFB52_00600"
     CDS             5106..7805
                     /locus_tag="VFB52_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04861.1"
                     /translation="MLAAVVLTTLFGAAVAGPASADFGIKSWQAGTCNKPDVLFVYQC
                     TYSAPNIDFYHQAAGHPVAGGTDFEVNTGGLGTPEGTLQQVRVDLPEGLNVNPQATPQ
                     CAKATFEANPADCGPSKVGVSVVYSITSLLPLPLEFPVYNLVPDQGHPALFGFNANVA
                     LLGIDEDVYLQADIDWSGDFHEGFTIQVPANPPLVRNRLVFEGTKGNSFITLPSPCNG
                     DTTTILTLTSAEGPTDTEATTPAGPGNPVPIDGCEKVPFEPDFEATAGAVTDSPAAVS
                     ATLKVPQSSPDNAMEINSSTIKRAAVSLPVGLGLNPAGAEPLAFCKDAQFPDGEGQRG
                     AISCPAKSQIGTVSIQTPVLPKDSLPGKVFLAEQKSRDPESGQLYRIFINAVSERYGV
                     DVRLQGNIKADAKTGRLTAIVNDAPQVTFSEFTLNFEGGEQAPLTSPPICGPNPTGGS
                     VTPWSGNADAHPSSPVTLSKAPGGGPCAKTMAERPFSPGFKASPGTDKVKTYTNFTAS
                     FSRPQGQQELKLVDITLPPGATAKLKGVPYCKPGEIADAEQRSGEAERKNPSCDKDSR
                     VGVAKVSAGTGSKPLQIDGDVYLAGPYKGAPLSLVVVTPAVAGPFDLGNVVVRVALNL
                     GPETARINPVALIPDVFGGAKLDIRSIFVNVNRKEFALTGTNCRKVATEGIVGGGGAD
                     PTNPATFSAFKVSEPFQANGCKKLKFKPKLKLRLFGKTFRNKNPRLRAALTTRPGDAN
                     IKRASVALPNAIILDQANLANICTRAQFAAEQCPKKSVYGKARAFTPLLGKPLEGPVY
                     LRSSNNTLPDLVAHLQGQVTIDLVGRIDSYKGGIRTTYDTVPDVPVTKFVLTLPGGKH
                     GLLVNSENLCKKAIRATVKIKGQNGKKANSKPKMRTPCGKKGK"
     gene            7990..10917
                     /locus_tag="VFB52_00605"
     CDS             7990..10917
                     /locus_tag="VFB52_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04862.1"
                     /translation="MLATVGFAQADTTDIIQPQNETSDQGFQSGTCLANEEPAPPNKA
                     CSPETPGLFFTQAAGHPPLGFTQYIIEHEPFTPLPSPPFPPGSLLAPIPGEDIKEHTI
                     KTLRTDLPPGLTVNPEATPSRCPLAAFENKVGENFVPTCDPSTKVGEDRVTLVTNKNE
                     VEIAPGFKPPKGFVIPPTEGATKVPIYNLEPEPGEPAKLGFIINGKVRVFLETEVAWE
                     NDFHESFRIKLANTAEASGLSTLISRLMSNGRSGDETYLTNPTTCFDPNEPEFETLYS
                     TWFRAESYGEPNPTFPIGSTPVEAKLPLVAPGERIQQTGCDDIPFEPTIETSVTTTEV
                     DSPTGGTIELKLPFDPAEEGGEGISQSHLRRAEVTLPKGMGLNPSGSVGLVACSDAQF
                     KKGVRVNSNECPTASKVGHAVIESPPLHEPLEGDIYVGEQKSMDPTSGEQFRILVEAK
                     SETEGIVVRLLGNVFADPVTGQLTGYFTDQLTGEFAGKLPDGLPQVPFESVKLTFDES
                     KKVLTSPPTCSPADTNGEMEPWARPGTSEPVSDSFTLTAVPGGGTCPLTLAERKFTPA
                     YSAASDNAKAGSYSPFRVHIGRADGQQEVKGVTVTLPKGLTGKLAGIPYCPEATIAAA
                     AAAAGKSGTTCPAASRLGAATTESGTGAAPLKLGGDAYLAGPYKGAPLSMVVVTPAVS
                     GPFDLGTVVVRVALQVAPETAQITAVSDLIPDVFGGVKLDIRSINVDVDRPEFMKNPT
                     SCAAQATNGTLNGGGGDPANPAAFSAYPVSSPYQANECKSLGFKPKFFTKLTGPVKRN
                     GNPKLRAIVEARGNDANITRTALTLPSSLLLDNDHIGTVCTRAQLASKTCPPNSVYGQ
                     AEATTPLLDGKLKGPVYLVANPETTLPNLLADLQGQVNIQLRGVISSKRGGLKTVFNG
                     LPDVPVKKFVLNMKGGKKSLLVNSQNICKNPQLAVLNIKGQNGKLVKKNKFRLNTASC
                     KKK"
     gene            10969..>11989
                     /locus_tag="VFB52_00610"
     CDS             10969..>11989
                     /locus_tag="VFB52_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO04863.1"
                     /translation="MRPSLSVVIVAWNSLAELERTLPALLAELGPEDELIVVDNDSSD
                     ATLARLPELAPAARLVRMGRNVGFAAGVNAGAAEARGDLLVMLNPDAAPAPGFGEAIR
                     RPWREGRGWAAWQALVAEAGGTTINSAGNPLHFTGIVWAGMHGEPLAAAPPAGEVTVL
                     SGACLAIPRATWEEIGGFPKQFFLYHEDVDLSLRLRLRGGTLGIEPAAIVAHDYEFGA
                     SAGRKMRWLERNRLAFLVRAYPAPLLVLLAPALIATELALLFVSAAGGWGGQKLAANW
                     EALRWLPRLLLERRRIQRTRTVASAEFAGWLSADLDSPFIAGPARSWPVRMALRAYLG
                     AVRALL"
BASE COUNT         2150 a         4307 c         3839 g         1693 t
ORIGIN      
        1 agccgtcgcc ttcgggcagt gccagcacct cctcgccgta ctcggcgacc agcgcgtcct
       61 tgatctgctc cttgctctcg ccttccgcga ccagccgttt gatgaagacc ttctggcgca
      121 gggcctgcgg cgattcggcc agttcgagca gggtgccgca gaccgggcac atcacttcgc
      181 cttcgatctc cgagagcgag gtctgcgtcg tcgccgcgcc tgcggtgggg gagagcgcca
      241 gcagcgccac aagcgcgccc agtgccaggc gcctcacgac ttccccttca acagcggctc
      301 gacttcgttt tcgaggtact cctcgctgac cgcgccgcgg gccagcaccc gcactttgcc
      361 ccgcgggtcg accaggtagc tctccggcac cccggtggcg ccgaactcgt cggcgaagtc
      421 cccgtcgcca tcgtgcagct gcggataggt gaggccgaat tcctcgacga agcgccgccc
      481 gtcgtcggag aggtcgctgc tgtcgattcc cagcaccgtg aacttctcac ccccgtactc
      541 gccctgcagc gcctccagcg ccggcgcctc ctcgcggcag gggatgcacc aggaggccca
      601 gatgttgacc agcacccagc tcccccggta gtcggccagc gattccgtcc cctcgccctc
      661 cagccgcggc agcgccgtgg tcggagcggg atcgccgggt tcgaggttcg accccttgct
      721 gagcaggccg aagacgagca ggcccacgat ggccagcacg ccgacgacag ctaaggagga
      781 cagcacgagg ctggccctgg aggaacgagg tctcacggac cgcgaaggtt agatatcgcc
      841 cgcccccggc agcctcccgc tcccgcctgc tccgcataca cttgccccag cgcggacgta
      901 gctcagttgg tagagcgcaa gcttcccaag cttgaggtcg cgggttcgac ccccgtcgtc
      961 cgcttgtcga cgaccagcag gcccaaggcg agcacgccga cccagaaggc ggcggcgccg
     1021 agggtgaggc gttgttggag gccgtagttg gcgtcgaggt cgctggagtc ggtggcgaag
     1081 aagagggcgg tccagatcgc gaggccgatc aggccggcgg cgagggagag caggtcgacg
     1141 cgccaccagc ggcggtcgcc gtggaagcgc caggccatcg cgaagacgga gagcatcagg
     1201 gcggccgagc cgacgaagta ggtccagccg tgggcgtagt ggcgccagga gagggtgccg
     1261 gcgaggtcgc gggcgtggca ggcgggatcg atcgtggtct ggcagtcggc ggggaagggg
     1321 aagccgacga ggatctgggc gaggccgcag agcgcgatca gcaccgggcc cagatagccg
     1381 cgcaggccgc cggggccgtc gagggcaagg gcgtaggcgg cggcgataaa ggaagagccc
     1441 cagaccgcca ccgcgaggtc gaacgcccag ggatgagcgg cggtctgggc gccgagcaag
     1501 ctgatcgcgt cgcgggtctc gctgtagccg ggctcgatca ggctggcgac gacgacgaag
     1561 acgacccagg ccagctggcc cgccagcgag gcgagcgcga gacggcgtat gtgcgcacgg
     1621 cgctgctccc cctgcatccc ccgaccacgt taggccgggg gcgggagcgg atcaagttcc
     1681 gcggggcttt aggcggcgac agcctgaggt tgaagttgag cgttagggtt tgcttcggtc
     1741 ttggcggcaa cgccgggcaa gcagtagaag tgctctgtgg cgccgcgcac ctgacgctgt
     1801 ttgaccatgt cgatcaggcc ggatttcgcc agctcggtga cgtggtagtt ggtgttgctg
     1861 aggggtatct gcagctcctg actgagctcg gaaggggaga gcgtgcgccc cccgtccatg
     1921 ccgaggattt cgaggatcga gacccgcagc gggtgggcgg tggagcgggc gaggtgctcc
     1981 cagtccaccg gcagcgaggc cgatccgttt tcaacggcac gcagggcgcc gttgccgttc
     2041 gctgagtgtc cgttggtaag cgacatgcga gccatattac ccaaaaatgt tgatttggat
     2101 catcaatttc gggtagagtc caaatcggga tgcttgattg gaggggaaat ctgcttgtcc
     2161 ggggaggccg aggcctggtg ctggtcgcgt tcgcggccgc gttcatggct ccgagcacgg
     2221 tggcggccag caccggtgac gtaatcgctc cccagcaccc gcccgcctac accgccgacg
     2281 acggctggca ggcaggaacc tgcaatgaag aaccacccga aagcaccgag gtctgctcgg
     2341 tcgagacgcc ggaccagttc ttcgaagggg ccgccgcgca cccgaactgg ggcttcaccc
     2401 agttcatcgt caaacacgaa gcgcccggcg aaaccccggt cgccgaactg aaacgggtgc
     2461 tggtcgacct tccggtcggg ctcagcgtca acccgggcgc gacgccgcgc tgcccgctcg
     2521 ccaccttcga agccggcgcc ggcggctgcc cgcccgcctc gcaggtcggc gaaagcatgg
     2581 tcaccgcctc gatgccgctg ctcggcaccc cgatcgcgcc gattcccggg gtcaccgagg
     2641 tggccgtcta caacctggtc cccgaagacg gcgagccggc ccgcttcggt ctcgaactcg
     2701 ccggcaacga agtcttcctc gaggcggacg tcgagtggga gggcgactac cacgagtact
     2761 tcgaaatcga ggtgccgaaa gcgctgccgc tggaaatccc cctcctggtc caggcgctga
     2821 tcctcaaaaa ccggttggtc ttcgacgggc gctccggcga cggcactttc atcaccaccc
     2881 cgagcacctg cctcggcgaa gcgggcgaag gcccgggcaa atcgggctcc atctactcga
     2941 cctggctgcg cgccggctcc tacgacgaat tcgaagaacc gggctacgtg ttcccgacca
     3001 gcgccgagcc cgccttcgag tcgccgatcc cacccggcac ctcgccgaaa gaatgcgact
     3061 cgatccccta cgacccgacg atcgcggtcg agcccggcac cgccgaaacc aactcgcccg
     3121 ccgccgcctc ggttgagatc gaggtcccgc acctgctccc cgactacggc gaaggggaaa
     3181 acgaacagga cagctcggtg accaaagcct cgaccgtgac cctgccgctg gggatgggga
     3241 tcaacccgtc cgccgctaac ggcctcgaga cctgcaccga tgcgcagttc ggcaagggca
     3301 cgcgcaaccc gatcgcctgt ccgccggcct cgaaagtcgg caccgtgacg gtcaaatccg
     3361 cgccgctccc cgaaggtgac ctcgagggca gcgtctacgt cggcgagcag ctcagccgcg
     3421 acccgctctc cggtgaggag taccggatct tcatcgccgc cgagtcggcc cgctacggga
     3481 tctcggtgcg gctggtcggc aaggtcaaag cagacccggt caccggccag ctctcgacca
     3541 cgatctccga agtcccgcag gtccccttca cctccttcga cctcgacttc aacggcggcg
     3601 cgcacgcggt gctcagcagc ccgccgacct gcggcccgaa caaaaccagc accgagatgg
     3661 tcccctgggc gccgatcgcc gccgcggcgc ccggcgacga gttcgaactt accgccaccc
     3721 cgggcggggg cgagtgcgcg aaaacgatgg ccgcccgcgc gttcgccccc gcttttgcgg
     3781 tcgccccgaa agcaaacacc gccggcgcct tcagcccgct ctcgatgcgg atcgcccgca
     3841 gcgacggcca gcaggagctg aaaggcgtcg acgtcaccct gccgcccggg atgaccggca
     3901 agctcgccgg catctcctac tgctccgagc aggcgatcgc ggcagccgcg gccaactcgg
     3961 gcaaagccga gaaggcggcc tcgacctgcc cggccgcgag cctcgtcggc agcgccacga
     4021 tcgacgccgg caccggtacg gacccgctgc ggatcggtgg caaagtcttc ctctcaggcc
     4081 catacaaagg ggcccccctc tccctggcag ttgtcacccc cgccacggcc gggccgtttg
     4141 atctcggcac ggccgtggtg agggtggcac tgttcctcga cccggaaacg gcgcagatcc
     4201 acgccgtctc cgacccggtc cccgacgtct acggcggcac ccagctcagc gtccgcagcg
     4261 tcgacgtcaa cctcgaccgc aaagacttca ccctcaaccc gaccagctgc gagccgctgg
     4321 ccggcggcgg cgccctgcgc ggcggcggcg ccaaccccgc cgacccggcg ctgttcagcg
     4381 ccttcccggt cgcgatcgcc ttccagacca gcaactgcga ggcgctgaag ttccgcccga
     4441 agctgaccac ccgcctgttc ggcggcaaga aatcgacgaa acgcggccag gagccgaaat
     4501 tccgcgccgt gctcaccgcc cgcggtggcg acgccaacat cggccgcgcc gcgctcaccc
     4561 tctcgcgcgc gctgatcctc gaacagggcc acatccgcac gatctgcaga aacgcggagc
     4621 tggccgccgg cgcctgcccg cagcgctcga tctacggcta cgcgatggcc gaatcgccgc
     4681 tgctcgacga gccgctcagc ggtccggtct acctcgtccc gacgaccagc gacagtggcc
     4741 tgcccgacct gcttgccgac ctgcgtggcc aggtcaacat ccgtctccgt ggccagatcg
     4801 gggccgccaa ggcgcgtctc aaaaccgtct tcccgatggt ccccgacgtg ccggtgagca
     4861 agttcgtgat cacgatgaag ggcggcaagc gcgggctgct gaccaacacg cgcgacctct
     4921 gtctgaagcc ggtctactca ttcctcaact tcaaagccca gaacggcaag aagctgaaga
     4981 agaagaaact gccgctgcgc gtccccggtt gcaagggcgc caagaagcgc gccaagggct
     5041 aacggggaac gcgcatgttt tccctgggac cgggtatgcc cggcggcatg aggagatcga
     5101 tgggggtgct cgcagccgtt gtcctgacga cgctgttcgg ggcggcggtc gccgggccgg
     5161 ccagtgccga cttcgggatc aaaagctggc aggccgggac ctgcaacaaa cccgacgtcc
     5221 tcttcgtcta ccagtgcacc tactcggcgc cgaacatcga cttctaccac caggccgccg
     5281 gccacccggt cgccggtggc accgacttcg aagtcaacac cggggggctc ggtacgcccg
     5341 aaggcactct gcagcaggtc cgcgtcgacc tcccggaggg gctcaacgtg aacccgcagg
     5401 cgaccccgca gtgcgcaaaa gcgaccttcg aagccaaccc cgccgactgc ggcccgagca
     5461 aagtcggggt cagcgtcgtc tactcgatca ccagcctgct gcccctaccg ctcgaattcc
     5521 cggtctacaa cctcgtcccg gaccagggac acccggcgct cttcggtttc aacgccaacg
     5581 tcgccctgct cggaatcgac gaggacgtct acctgcaggc ggacatcgac tggtccggcg
     5641 acttccacga ggggttcacg atccaggttc cggccaaccc gcccctggtt cgcaaccggc
     5701 tcgtcttcga agggacgaaa ggcaacagct tcatcaccct gcccagcccc tgcaacgggg
     5761 acacgacgac gatcctcacc ctgacctccg ccgagggccc gaccgacacc gaggcgacga
     5821 ccccggccgg ccccggcaac ccggtcccga tcgacggctg cgagaaagtc ccgttcgagc
     5881 ctgacttcga ggccaccgcc ggcgcggtca ccgactcgcc cgcggcggtc tcggcgacgc
     5941 tgaaggtgcc gcagagctca cccgacaacg cgatggagat caactcctcg acgatcaaac
     6001 gcgccgccgt ctcgctgccg gtcggcctcg gcctcaaccc cgccggtgcc gagccgctgg
     6061 ccttctgcaa agacgcccag ttccccgacg gcgagggcca gcgcggggcg atctcctgcc
     6121 cggcgaaatc gcagatcggc acggtctcga ttcagacccc ggtcctgccg aaagactcgc
     6181 tgccgggcaa ggtcttcctc gccgagcaga agagccgcga ccccgaatcg ggccagctct
     6241 accggatctt catcaacgcc gtctccgagc gttacggcgt cgacgtgcgc ctgcagggca
     6301 acatcaaagc cgacgcgaag accggtcgcc tgaccgcgat cgtcaacgac gccccgcagg
     6361 tcaccttcag cgaattcacg ctcaacttcg agggtggcga gcaggcaccg ctgaccagcc
     6421 cgccgatctg cggcccgaac ccgaccggcg gctcggtcac gccctggtcg ggcaacgccg
     6481 acgcgcatcc ctcgagcccg gtgacgctga gcaaagcgcc gggtggcggg ccgtgcgcga
     6541 aaacgatggc cgagcggccg ttcagtccgg gcttcaaagc cagccccggc accgacaaag
     6601 tcaagaccta caccaacttc accgcctcgt tcagccgccc gcagggccag caggagctga
     6661 agctggtcga catcaccctg ccccccggtg cgaccgcgaa gctgaagggc gtcccctact
     6721 gcaaacccgg tgagatcgcc gacgccgaac agcgctccgg cgaggccgag cgcaaaaacc
     6781 cgagctgcga caaagacagc agggtcgggg tcgcgaaagt gagtgccggc accggctcga
     6841 aaccgctgca gatcgacggc gacgtctatc tcgccggccc ctacaagggc gcgccgctct
     6901 cgctggtcgt cgtcaccccg gcggtcgccg ggccgttcga cctcggcaac gtcgtcgtcc
     6961 gggtcgcgct caacctcggg ccggagacgg cgcggatcaa cccggtcgcg ctgatccccg
     7021 acgtcttcgg cggcgccaag ctcgacatcc gctcgatctt cgtcaacgtc aaccgcaaag
     7081 agttcgcgct gaccgggacc aactgccgca aggtcgcgac cgagggcatc gtcggcggtg
     7141 gtggggcgga cccgaccaac ccggcgacct tctcggcctt caaagtctcc gaacccttcc
     7201 aggccaacgg ctgcaaaaaa ctgaagttca aaccgaagct gaagctgcgc ctgttcggca
     7261 agaccttccg caacaagaac ccgcgtctgc gcgcggctct gaccacgcga cctggcgacg
     7321 ccaacatcaa gcgcgcctcg gtggcgctgc cgaacgcgat catcctcgat caggccaacc
     7381 tggccaacat ctgcacccgt gcacagttcg ccgccgagca gtgcccgaag aagtcggtct
     7441 acggcaaggc ccgcgcgttc acgccgctgc tcggcaaacc gctcgaagga ccggtctacc
     7501 tgcgctcctc gaataacact ttgcccgacc tcgtcgccca cctgcagggc caggtcacga
     7561 tcgacctggt cggccgcatc gacagttaca agggcggcat ccgcaccacc tacgacaccg
     7621 tgcccgacgt tccggtgacc aagttcgtac tgaccttgcc gggcggcaaa cacggcctcc
     7681 tggtcaattc ggaaaacctt tgtaaaaaag cgatccgggc gaccgtgaag atcaaaggcc
     7741 agaacggcaa gaaggccaac tcaaagccga agatgcgcac gccctgcggc aagaagggca
     7801 agtagtcacc ttctccagtc gcattcggga acgcccttga cttgtggctg gctgaccggt
     7861 tagcgtcgtt tgcgggagaa gtacggggaa cgccggggca gggacggcga tgggtctgca
     7921 tgtccaaacg aggagggatc gatggccagg cgcacgattg gggttctggc gacggcatta
     7981 ggaatgctgt tgctcgcgac tgtggggttc gcgcaagcgg atacgaccga cattattcag
     8041 ccgcagaacg aaacgtctga ccaggggttc cagtcgggca cctgcttggc gaacgaagaa
     8101 cccgcgccgc cgaacaaagc gtgttcgccg gaaacgccgg ggctcttctt cacccaggcg
     8161 gctggccatc cgccgctcgg cttcacccag tacatcatcg agcacgaacc cttcacgccc
     8221 ctgccatcgc caccgtttcc gccggggagc ctcctcgctc caatccctgg tgaagacatc
     8281 aaagaacaca ccatcaaaac gctgcggacc gacctgccgc cggggctgac cgtgaacccg
     8341 gaggcgactc cctcgcggtg tccgctcgcg gctttcgaaa acaaagtcgg ggaaaacttt
     8401 gtgccgacct gcgatccgag caccaaagta ggcgaagacc gcgtcaccct cgttacgaac
     8461 aaaaacgaag tcgaaatcgc ccccggtttc aaaccgccga aagggttcgt catccccccg
     8521 accgaagggg cgacgaaagt accgatctac aatttggagc ccgaacccgg cgaaccggcc
     8581 aaactcggct tcatcatcaa cggcaaagtc agggtcttcc tcgaaaccga agtcgcctgg
     8641 gagaacgact tccacgagag cttccggatc aaactcgcga acacggcgga agcgagcggg
     8701 ctctccaccc tgatcagccg cctgatgagc aacggccgct ccggcgacga aacgtacctg
     8761 actaacccga ccacctgttt cgacccgaac gagccggagt tcgaaacgct gtactcgacc
     8821 tggttccggg ccgagtccta cggcgaaccg aacccgacct tcccgatcgg ctcgaccccg
     8881 gtcgaggcga aactgccgct cgtcgccccg ggtgaacgga ttcaacagac cggctgcgac
     8941 gacatcccgt tcgaaccgac gatcgagacc tcggtgacga ccacggaggt cgactcgccg
     9001 accggcggca cgatcgagct gaaactgccc ttcgatcccg ccgaagaagg tggcgaaggg
     9061 atctcgcagt cgcacctgcg ccgcgctgaa gtgacgctgc cgaagggcat gggtctcaac
     9121 ccgtccggtt cggtcggcct cgtcgcctgc agcgacgccc agttcaagaa gggcgtccgc
     9181 gtcaacagca acgaatgccc gacggcttcg aaagttggcc acgcggtgat cgagtcgccg
     9241 ccgctgcacg agcctctcga gggcgacatc tacgtcggcg aacagaagag catggacccc
     9301 acctcgggtg aacagttccg catcctggtc gaagccaaga gcgaaacgga gggcatcgtc
     9361 gtccgtctgc tcggcaacgt cttcgccgac ccggtgaccg gtcagctgac cgggtacttc
     9421 accgaccaac tgaccggaga gttcgccggc aaactgccgg acggcctccc gcaggttccg
     9481 ttcgagtcgg tgaaactcac cttcgacgag tccaaaaaag tcctgacgag cccgccgacc
     9541 tgttcgccgg cggacaccaa cggtgagatg gaaccgtggg cgcgccccgg caccagcgag
     9601 ccggtcagcg actcgttcac gctgaccgcc gtcccgggtg gcggtacctg cccgctgacg
     9661 ctggccgagc gtaaattcac cccggcctac agcgccgcgt ccgacaacgc caaagccggc
     9721 tcctacagcc cgttccgcgt ccacatcggc cgcgccgacg ggcagcagga ggtcaagggt
     9781 gtgaccgtca ccctgccgaa gggcctgacc ggcaagctcg ccgggatccc gtactgcccg
     9841 gaggcgacga tcgcggcggc tgccgccgcg gccggcaaat cgggcacgac ctgcccggcg
     9901 gcgagccggc tcggcgccgc gaccaccgag tcggggaccg gcgccgcgcc gctgaaactg
     9961 ggcggcgacg cctacctggc gggcccctac aaaggcgcgc cgctctcgat ggtggtggtc
    10021 acgccggcgg tctccggacc gttcgacctc ggcaccgtgg tcgtccgcgt ggcgctccag
    10081 gtcgcacctg aaaccgccca gatcacggcg gtctcggacc tgatcccgga cgtgttcggc
    10141 ggtgtgaagc tcgacatccg ctcgatcaac gtcgacgtcg accggccgga attcatgaag
    10201 aacccgacca gttgcgcggc gcaggccacc aacgggacgc tcaacggcgg cgggggcgac
    10261 ccggccaacc cggccgcgtt ctcggcctac ccggtcagct cgccctacca ggcgaacgaa
    10321 tgcaagagcc tcggcttcaa gccgaagttc ttcaccaagc tgaccggccc ggtcaagcgc
    10381 aacggcaacc cgaagctgcg ggcgatcgtc gaagcccgcg gcaacgacgc caacatcacc
    10441 cgcacggcgc tgaccctgcc gtcgtcgctg ctgctcgaca acgatcacat cggcacggtc
    10501 tgcacgcggg cccagctcgc ctcgaagacc tgcccgccga actcggtcta cgggcaggcg
    10561 gaagcgacga cgccgctgct cgacggcaag ctgaaaggtc cggtctacct ggttgccaac
    10621 ccggaaacga cgctgccgaa cctgttggcc gacctgcagg gacaggtgaa catccagttg
    10681 cgcggcgtga tcagctcgaa gcgaggcggc ctcaaaaccg tcttcaacgg gctgcccgac
    10741 gtgccggtga agaagttcgt cctcaacatg aagggcggca aaaagagcct gctggtcaac
    10801 tcgcagaaca tctgcaagaa cccgcagctg gcagtgctca acatcaaggg ccagaacggc
    10861 aagctggtca agaagaacaa gttccgcctc aacaccgcct cctgcaagaa gaagtagggc
    10921 gcgcatgacg ctcgcaccgg cgcggaaggc cgactagcct ccgcgccggt gcgtccgtcg
    10981 ctctccgtag tcatcgtcgc ctggaacagc ctcgccgagc tcgagcggac gctgcccgcg
    11041 ctgctcgccg agctcggccc cgaggacgag ctgatcgtcg tcgacaacga ctccagcgac
    11101 gcgaccctcg cccggctgcc ggagctggcg ccggccgcgc ggctggtgcg gatggggcgc
    11161 aacgtcggct tcgccgccgg ggtcaacgcc ggcgccgcgg aagcgcgcgg cgatctgctg
    11221 gtgatgctga accccgacgc cgcgccggcc cccgggttcg gcgaggcgat ccgccgcccc
    11281 tggcgcgagg gccgcggctg ggcggcctgg caggcgctgg tcgccgaggc gggggggacg
    11341 acgatcaact cggccggcaa cccgctgcac ttcaccggga tcgtctgggc cgggatgcac
    11401 ggcgagccgc tcgccgcggc gccgccggcc ggcgaggtca cggtcctctc cggcgcctgt
    11461 ctggcgatcc cgcgggcgac ctgggaggag atcggtggct tcccgaagca gttcttcctc
    11521 taccacgagg acgtcgacct ctcgctgcgg ctgcggctgc gcggcggcac actcgggatc
    11581 gagccggcgg cgatcgtcgc ccacgactac gagttcggcg ccagcgcggg gcgcaagatg
    11641 cgctggctgg agcgcaaccg gctcgccttc ctcgtccgcg cctacccggc gccgctgctg
    11701 gtcctgctcg ctccggccct gatcgcgacc gagctcgccc tgctcttcgt ctccgccgcc
    11761 ggtggctggg gcggccagaa gctggccgcg aactgggaag cgctgcgctg gctgccccgc
    11821 ctgctgctcg agcgccgccg catccagcgc acccgcacgg tcgccagcgc cgagttcgcc
    11881 ggctggctca gcgccgacct cgactcgccc ttcatcgccg gaccggcccg cagctggccc
    11941 gtgcggatgg cgctgcgcgc ctacctgggc gccgttcgcg cgctgctgc
//
LOCUS       DASRIG010000028         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3138588, whole genome shotgun sequence.
ACCESSION   DASRIG010000028 DASRIG010000000
VERSION     DASRIG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138588"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFB52_00615"
     CDS             complement(<1..478)
                     /locus_tag="VFB52_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZO04864.1"
                     /translation="MQKLAEICIQRPVFAAMIILSLVVVGAASYFRLGVDRFPSIDLP
                     TVTIRTTLPGASPEEAESELSRPIEDVVNTVEGVEQLRSVSGQGRSFVIATFNLDRDI
                     EAAAQDVRDRVQSVLRQLPPETDPPIVQKADSDQAPVMAIALSGDRSLRELTELADK"
     gene            complement(607..1824)
                     /locus_tag="VFB52_00620"
     CDS             complement(607..1824)
                     /locus_tag="VFB52_00620"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO04865.1"
                     /translation="MGRPIWKLSLALCLSLTTLWLTACGSGEPQAAAPRGGAGGLPTK
                     AVRLTPVVERPLARTANAPGTLAADEQATLSFKVPGRLNRLGIDLGSIVRQRQAIAQL
                     ETQEFKVRVEQSGAALQQARARLGLPPEGEDDRIKTEETALVRQARAVLEESRLNRER
                     TIRLVREGVLPQAELDRLESAFKVADSEYQNAIEEVRNRQAVLLQRRSELAIARQQLA
                     ETTLYAPFDGAVRERRANLGEYLTAGAPVAIIVRLHPLRLRVEVPEREVRGVRVGLPV
                     RVTVEGDTNVYSGRVARLSPAFEEQSRTLIVEAEVDNQQGRLRPGSFAKAEIQTSTDA
                     GVLTVPAAAVVTFAGIEKVFTVKDGKAVEHSVVLGRREADWVEVTAGIQANDQVVATP
                     GNLVSGQPVTIDQ"
     gene            2019..>2758
                     /locus_tag="VFB52_00625"
     CDS             2019..>2758
                     /locus_tag="VFB52_00625"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZO04866.1"
                     /translation="MTKIIAPALLALTIAWWAAPAPFHRAGLIFPLEHWHNHASAIVE
                     CPNGDLLVCWFHGSGERTADDVKVEGARLKKGAAAWGPRLTLADTPGYPDTNCTLFID
                     PRQRLWLLWPTILANEWPTALMKYRISSNYQGEGAPRWEASEVLHVTPGPEFEQAVKV
                     AMDEVESRIGSETAGRRAQIQEYVARQRRQAADKLSRRLGWMTRAHPFVLDGQRLIVP
                     LYSDGFSFSLMAITDDWGQTWHASAPLIG"
BASE COUNT          425 a          954 c          944 g          435 t
ORIGIN      
        1 tcttgtcggc cagctcggtc agctcgcgca gcgagcggtc gcccgagagg gcgatcgcca
       61 tgaccggggc ctgatcgctg tccgccttct ggacgatcgg cgggtcggtc tcgggcggca
      121 gctggcgcaa gacgctctgg acgcggtcgc gcacgtcctg ggccgcggcc tcgatgtcgc
      181 ggtcgaggtt gaaggtggcg atgacgaacg agcggccctg ccccgagacc gagcgcagct
      241 gctcgacacc ttcgaccgtg ttgacgacat cctcgatcgg ccgcgagagc tccgactcgg
      301 cctcctcggg cgaggccccg ggcagcgtcg tccggatggt caccgtgggc aggtcgattg
      361 acgggaagcg atcgacgccg agccggaagt agctcgccgc cccaaccacg accagcgaga
      421 ggatgatcat ggccgcgaag accggccgtt ggatacagat ttcggctagc ttctgcataa
      481 aactacgacc ctggaatcaa gacggcgaaa gatgtccagc agtttcaata aattcagctt
      541 tcagccgtca gccagacagg cggcgccggt gtggctgacc gctgaaagct gacagctgac
      601 tgctgctcat tggtcgatcg tcaccggctg ccccgagacg aggttgccgg gggtggcgac
      661 gacttgatcg ttggcctgga tgcccgcggt gacctcgacc cagtcggcct cgcggcgccc
      721 gaggacgacg ctgtgctcga cggccttgcc atccttgacg gtgaagacct tttcgatccc
      781 ggcgaaggtc accacggccg cggccgggac cgtcaggacg ccggcgtcgg tgctcgtctg
      841 gatctcggcc ttggcgaacg agcccggccg cagccgcccc tgctggttgt cgacttcggc
      901 ctcgacgatg agggtccgac tctgctcctc gaaggccggg ctgaggcggg cgacgcggcc
      961 cgagtaaacg ttggtgtcgc cctcgaccgt gacgcgcacc gggagcccga cgcggacccc
     1021 gcggacctcg cgctccggca cctcgacccg cagccgcagc gggtgcagcc ggacgatgat
     1081 cgccaccggg gcgcccgccg tgaggtactc gccgaggttg gcgcggcgct cccggacggc
     1141 gccgtcgaag ggcgcgtaga gagtcgtctc ggccagttgc tggcgggcga tggccagctc
     1201 ggagcggcgc tggaggagca ccgcctggcg gttgcgcacc tcctcgatcg cgttctggta
     1261 ctcgctgtcg gcgaccttaa aggccgactc cagccggtcg agctcggcct gcggcaggac
     1321 gccttcgcgg accaggcgga tcgtgcgctc gcggttgagc cgggactctt caaggacggc
     1381 ccgggcctgc cgcacgagcg ccgtctcctc agtcttgatc cggtcgtctt cgccctccgg
     1441 cgggaggccc agccgcgcgc gcgcctgctg gagcgcggcc ccggactgct cgacgcggac
     1501 cttgaattcc tgggtctcga gctgcgcgat cgcctgccgc tggcggacga tgctgccgag
     1561 gtcgatcccg agtcggttga gccgcccggg gaccttgaag ctcagcgtgg cctgctcgtc
     1621 ggccgcgagc gtccccggcg cgttcgccgt gcgcgccagc ggccgctcga cgaccggcgt
     1681 cagccgcact gccttggtcg gcagcccgcc tgcccccccc cgcggggcgg ccgcctgcgg
     1741 ctcaccgctg ccgcaggcgg tcagccagag tgtcgtcagg gagaggcaaa gtgcgaggct
     1801 cagtttccag ataggtcttc ccataaattc tattaaggag caacgaccgc acggtcacgc
     1861 cgaccaggag ggaggtggtt cggggcatga caggagatcc acaatttctt gaaattaata
     1921 acgccgggca ttataactcg cggcggcgct tcttgcacgg agcgagacct gaaatcgccc
     1981 gggcgacgcc ttttcgcccc ggcctctctg agagacttat gaccaaaatc atcgcccccg
     2041 cgctcctcgc cctgaccatc gcctggtggg ccgcgccggc ccccttccac cgagccggac
     2101 tgatctttcc gctcgagcac tggcacaacc atgcctcggc gatcgtcgaa tgcccgaacg
     2161 gcgacttgct cgtctgctgg ttccacggct cgggcgagcg cacggccgac gacgtcaagg
     2221 tcgagggggc gcgcctcaag aaaggggccg ccgcctgggg gccgcgcttg acgctcgccg
     2281 acacgcccgg ctaccccgac accaactgca cgctcttcat cgacccacgg cagcgcctct
     2341 ggctcctctg gccgacgatc ctcgctaatg agtggccgac ggcgctcatg aaataccgca
     2401 tctcgtccaa ctaccagggc gagggcgcgc cgcgctggga ggcgagcgag gtgctccacg
     2461 tcacccccgg gccggagttc gagcaggcgg ttaaggtggc catggacgaa gtcgagtccc
     2521 gcatagggag tgagacggcg gggaggcggg cgcagataca agaatatgtc gcgcgccagc
     2581 gccgccaggc ggccgacaag ctctcgcgcc ggctcgggtg gatgacgcgc gcgcacccgt
     2641 tcgtcctcga cgggcaacgc ctgatcgtgc ccctctactc ggacggcttt tcgttctcgc
     2701 tcatggcgat caccgacgac tgggggcaga cctggcacgc gagcgcgccg ctcatcgg
//
LOCUS       DASRIG010000029         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1836542, whole genome shotgun sequence.
ACCESSION   DASRIG010000029 DASRIG010000000
VERSION     DASRIG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836542"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFB52_00630"
     CDS             <1..576
                     /locus_tag="VFB52_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO04867.1"
                     /translation="ITGEGEFNEVFINEARVPEENIVGSVGDGWGVAITTLMNERAGL
                     AFGAIAQIQNSLGRLAKLARETRVEGGSTAAEDTYYRQRIAQLFIEAETMRLNAYRGL
                     TKTMQSGIPGPEGSLGKWQWADINQDLTELALEIEGAYAALGRGSERALDSGAWQYSF
                     LRSRANSIEGGTTDILKNIIAERVLGLPRMR"
     gene            590..1693
                     /locus_tag="VFB52_00635"
     CDS             590..1693
                     /locus_tag="VFB52_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZO04868.1"
                     /translation="MYFDLTDEQQAIKATARDFLAARFKSDRIREIAASDDGTDPDGW
                     REMSELGWTGLAIPEQWGGQGLGIVDLAVLFEELGYALAPSPLLSNTVVGLALTLCGS
                     DDQRDRYLGAIASGERRAAPALWDAGSTATPGAFKMEAKADGDGVVLDGEKVLVMDAA
                     GAEHFLVATSDGRRHLVAADATGLTVIPETSIDTTRRLSSLRFEGVRVDAADTLPAAG
                     AEYEAVLDRMCVALAAESTGVAQRTLEMAVEYAKDRQQFGRPIGAYQAVSHRCAQMLL
                     ETENARSAVYGAAWAADAEPESLRVASSMAKAYASDAGWRVPDASIQVHGGIGFTWEH
                     DLHFFLKRGKANAGFYGDAKWHRERVADAVLAA"
     gene            1690..2721
                     /locus_tag="VFB52_00640"
     CDS             1690..2721
                     /locus_tag="VFB52_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO04869.1"
                     /translation="MSALDRISVAAIGTSVADLEAETLRAEAAGVECVWAPELFRSAV
                     TQAAYLAAKTTKIDVATGIVWAFTRSPFINAISALDIDEMSGGRFRLGMGAGVKRLNE
                     TWHNAAYGKPAPHLREAIEATRLIMQQASAGEPIRYEGEYYDIDIKGWVRPHPAARES
                     VPIYTAAVQAGMARMAGDVADGLIGHPIQSLRWIDEVVVDAFETGLSRSGRERSDFDY
                     LPTVCCAIDDDEGRAIEMARRTISFYATVKTYMPLWELHGFADNAVAAGDAFRRGDLA
                     AVPAAISDEMVETYCAAGPLDKVRARVEQTAERADGLFLTPPTYFISPEELSEFQNRI
                     IDAFGPASR"
     gene            2751..2999
                     /locus_tag="VFB52_00645"
     CDS             2751..2999
                     /locus_tag="VFB52_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZO04870.1"
                     /translation="MLERVRSIPEGHVASYGDVSPGAPRFAGTVLFGVEEPDLPWWRV
                     VRADGSLAKGARQRRLLIAEGVPFRGERVDMDAARVVP"
     gene            complement(3038..3562)
                     /locus_tag="VFB52_00650"
     CDS             complement(3038..3562)
                     /locus_tag="VFB52_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04871.1"
                     /translation="MSTLKKRRPSPAMVVAIIALIAALGGTAYAAQQINGGAIMKQTI
                     GGGKLKQKTLTGFQINTNKLGVVPTAKRATHTYWAVVNNPAGPANAALARASDTGITA
                     VEGNGGVSVVFPQNISGCANVAARNNAGTTVPGSGYAQTNTAAANANAVEVHTRDKDG
                     TNEDADFHLIVICP"
     gene            complement(3620..>4129)
                     /locus_tag="VFB52_00655"
     CDS             complement(3620..>4129)
                     /locus_tag="VFB52_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO04872.1"
                     /translation="LTHAHPDHQGVAKDVCEARNIPLACHADDVEAMEGKRPMQEASA
                     SNPINQVIKAIWQGPPYKVDRVLQEGDEVAGFRVVHAPGHARGEVIFFRDSDRVAICG
                     DVIRNMSYATALPGIKEPPDIFTADPAENRRSIRKLAELEPSLILPGHGPEVTDMQAF
                     YRFVSSLPG"
BASE COUNT          600 a         1424 c         1481 g          624 t
ORIGIN      
        1 atcaccggcg agggcgagtt caacgaggtc ttcatcaacg aggctcgggt ccccgaggag
       61 aacatcgtcg gcagcgttgg cgatggctgg ggggtggcga tcacgacgct gatgaacgag
      121 cgcgccgggc tcgccttcgg cgcgatcgcg cagatccaga acagcctcgg ccggctcgcc
      181 aagctggccc gggagacgcg ggtcgagggc ggctccacgg ccgccgagga cacctactac
      241 cgccagcgga tcgcgcagct cttcatcgag gccgagacga tgcggctgaa cgcctaccgc
      301 ggcctgacca agacgatgca gtcgggcatc ccagggccgg agggctcgct gggcaagtgg
      361 cagtgggccg acatcaacca ggacctgacc gagctggcgc tggagatcga gggcgcctac
      421 gcggcgctcg gccgcggctc cgagcgggcg ctcgacagcg gcgcctggca gtacagcttc
      481 ctgcgctcgc gcgcgaactc gatcgagggc ggcaccaccg acatcctcaa gaacatcatc
      541 gccgagcggg tgctcggcct gccgaggatg cgctaggggc agcgggcgga tgtacttcga
      601 cctcaccgac gagcagcagg cgatcaaggc gaccgcgcgc gacttcctcg ccgcccgctt
      661 caaatcggat cggatccgcg agatcgccgc cagcgacgac ggcaccgacc ccgacggctg
      721 gcgcgagatg tcggagctcg gctggaccgg cctcgcgatc cccgagcagt ggggtgggca
      781 ggggctcggg atcgtcgacc tcgcggtgct cttcgaggag ctcggctacg cgctcgcccc
      841 ctcgccgctg ctctcgaaca cggtcgtcgg cctggccctg acgctctgcg gctccgacga
      901 ccagcgcgac cgctacctgg gcgcgatcgc cagcggcgag cgccgcgccg cgcccgccct
      961 ctgggacgcc ggctcgaccg cgaccccggg cgccttcaag atggaggcga aggcggacgg
     1021 cgacggcgtt gtgctcgacg gcgagaaggt cctggtgatg gacgccgccg gcgccgaaca
     1081 cttcctggtc gcgaccagcg acggccgccg ccacctggtc gccgccgacg caaccggcct
     1141 caccgtgatc ccggaaacct cgatcgacac gacccggcgg ctttcctcgc tgcgcttcga
     1201 gggcgtgcgc gtcgacgctg ccgacaccct gccggctgcg ggcgccgagt acgaggcggt
     1261 gctcgaccgg atgtgcgtgg cgctcgccgc cgagtcgacc ggggttgccc agcgaacgct
     1321 cgagatggcg gtcgagtacg ccaaggatcg ccagcagttc ggtcgcccga tcggcgccta
     1381 ccaggcggtc tcgcaccgct gcgctcagat gctgctggag accgagaacg cgcgctcggc
     1441 cgtctacggt gccgcctggg ctgccgacgc ggagcccgag tcgctgcgag tggcctcctc
     1501 gatggccaag gcctacgcct ccgacgccgg ctggcgcgtc cctgacgcgt cgatccaggt
     1561 ccacggcggg atcggcttca cctgggagca cgacctccac ttcttcctca agcgcggaaa
     1621 agccaacgcc ggcttctacg gcgacgccaa gtggcatcgc gaacgggtcg cggacgcggt
     1681 cctcgctgcg tgagcgcgct cgaccgcatc tcggttgccg cgatcggaac ttcggtcgcc
     1741 gacctcgagg ccgagaccct gcgcgccgag gccgccgggg tcgagtgcgt gtgggcgccg
     1801 gagctgttcc gcagcgccgt cacccaggcc gcgtatctcg ccgccaagac gacgaagatc
     1861 gacgtcgcca ccgggatcgt ctgggccttc acccgcagcc ccttcatcaa tgcgatcagc
     1921 gccctcgaca tcgacgagat gtcgggcggt cgcttccgcc tcgggatggg tgccggggtc
     1981 aaacgcctaa acgaaacctg gcacaacgcc gcctacggca aaccggctcc gcacctgcgc
     2041 gaggcgatcg aggcgacccg gctgatcatg cagcaggcga gcgccggcga gccgatccgc
     2101 tacgagggcg agtactacga catcgacatc aaaggctggg tgcgcccgca cccggcggcg
     2161 cgcgagtcgg tgccgatcta caccgccgcg gtgcaggccg ggatggcgcg gatggccggc
     2221 gacgtcgccg acggcctgat cggccatccg atccagagcc tgcgctggat cgacgaggtg
     2281 gtcgtcgacg cgtttgaaac cggactctcg cgctcggggc gcgagcgcag cgatttcgac
     2341 tacctgccga ccgtctgctg cgcgatcgac gacgacgaag ggcgggcgat cgagatggcc
     2401 cgccgcacga tctccttcta cgcgaccgtg aagacctaca tgccgctctg ggagctgcac
     2461 gggttcgccg acaacgcggt cgccgccggc gacgccttcc gccgcggcga cctcgccgcg
     2521 gtgccggccg cgatcagcga cgagatggtc gagacctact gcgccgccgg gccgctcgac
     2581 aaggtccgcg cccgggtcga gcagacggcc gaacgcgccg acgggctctt cctgacgccg
     2641 ccgacctact tcatctcgcc ggaggagctg agcgagttcc agaaccggat catcgacgcc
     2701 ttcgggcccg cgtcgcgctg actcccagcg acggatcgcg cgaactggaa gtgctcgagc
     2761 gcgtgcgctc gatccccgag ggccacgtcg ccagctacgg cgacgtctcg cccggcgccc
     2821 cgcgcttcgc cggcaccgtg ctgttcggcg tcgaggagcc cgacctgccc tggtggcgcg
     2881 tggtccgcgc cgacggctca ctcgccaagg gcgcccgcca gcgccggctg ttgatcgccg
     2941 agggcgtccc attccgcggc gagcgagtcg acatggacgc cgcccgcgtg gttccctaac
     3001 cgtcgcaggg acggataagg aaccacgcga tgcgccgcta cgggcagatg acgatcaggt
     3061 ggaagtcggc gtcctcgttg gtgccgtctt tgtcgcgggt gtggacttcg accgcgttgg
     3121 cgttggccgc cgcggtgttg gtctgcgcgt agcccgagcc gggcacggtg gtgccggcgt
     3181 tgttgcgggc ggcgacgttt gcgcagccgg agatgttctg ggggaagacg acggagacgc
     3241 cgccgttgcc ctcgaccgcg gtgatgccag tgtcgctggc gcgggcgagc gccgcgttgg
     3301 ctgggccggc cgggttgttg acgacggccc agtaggtgtg ggtcgcgcgc ttggccgtcg
     3361 gcaccacgcc gagcttgttg gtgttgatct ggaacccggt gagggtcttc tgcttcagct
     3421 tgccgccgcc gatcgtctgc ttcatgatcg cgccgccgtt gatctgctgg gcggcgtagg
     3481 cggtcccgcc gagggcggcg atcagggcaa tgatcgcgac caccatcgca ggggacggac
     3541 gtcgcttctt gagcgtgctc atctggagag ctcccttctc ctctgtgggg ggcttatcgg
     3601 gccgtgcccg atatctcccc taacccggca gagacgaaac gaagcggtag aaagcctgca
     3661 tatcggtgac ctcggggccg tggccgggca ggatcaggct gggttccagc tcggcgagct
     3721 tgcggatcga gcggcggttc tcggccgggt cggcggtgaa gatgtccggt ggctccttga
     3781 tcccgggcag ggcggtggcg tagctcatgt tgcggatcac gtcgccgcag atcgcgaccc
     3841 ggtcggagtc gcggaagaag atcacctcgc cgcgcgcgtg gccgggggcg tggacgacgc
     3901 ggaagccggc gacctcgtcg ccctcctgga gcacccggtc gaccttgtag ggagggccct
     3961 gccagatcgc tttgatcacc tggttgatcg ggttgctcgc ggacgcttcc tgcatcggcc
     4021 gcttgccttc catcgcttcg acgtcgtcgg cgtggcaggc gagcgggatg ttgcgcgctt
     4081 cgcagacgtc cttggcgacg ccctggtggt ccgggtgggc gtgggtcag
//
LOCUS       DASRIG010000030         6382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_537942, whole genome shotgun sequence.
ACCESSION   DASRIG010000030 DASRIG010000000
VERSION     DASRIG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537942"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..849)
                     /locus_tag="VFB52_00660"
     CDS             complement(244..849)
                     /locus_tag="VFB52_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04873.1"
                     /translation="MRIWCATLAIVTAAAVMIGCGDDESPAPATSTSTTTRAQADGGE
                     DPSASPACRAVSKRRIEGEVAAAGGPARPLERSANDSLDLSICEYRERRGPDLYVSVT
                     LDTAPKTALRYYNLIAEGLQRATFEHIPDSTKPVGVRGVGNDRTHGGVGAYWTPYRSQ
                     LTAIDERTLVKVNFNVPGADPRTSRSAATVIARQALDSIRD"
     gene            1049..2188
                     /locus_tag="VFB52_00665"
     CDS             1049..2188
                     /locus_tag="VFB52_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04874.1"
                     /translation="MANPGELDASFAGDGKKAIDFGGTDAARVVQVQPNGRIIVAGGG
                     AAAVSFCVAGLRTNGAPDTTFGSGGKRVIDFGGDVESVEGAALQPDGKIVLAGGSDVR
                     VAVARLNPNGSLDTGFSGDGKKTFSWGAFSRASAVRVRPDGKIVLAGFSGPEGGNIEA
                     ARLNANGKLDATFGTGGKASVDFGGDDFGLAMARQADGRILIAGRSSAAGAVVARLRA
                     NGTLDADFDGDGRVTLPGGGSASAVLVQPDGRIVVAGNSNGNEVMTVTRLQPNGSPDA
                     TFDGDGTATIDFGSLSDLASDAVLQADGKIVVAGYTQADEDVAVARLNPDGSPDATFG
                     AAGKATVDFGVATFGNAVALQPNGRIVVAGQRTGGDDFAVARLLG"
     gene            complement(2228..3640)
                     /locus_tag="VFB52_00670"
     CDS             complement(2228..3640)
                     /locus_tag="VFB52_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04875.1"
                     /translation="MIPTGKATIRGVTALIAVGLVAGLATPARGSAAQELDHAKAAGI
                     AKLKKQAKQKTKKAVKKSLPGARTPGLSKPPSPPRVEVSNCAERSNGGRLTRFTCRWK
                     VSGELSGRVPVRCTGKATYKVRKKKVTKVSPCENREARQTPLLAAPHDVLFGYFEDFT
                     THGNLFGELGASGANTLREGIAWNVLEPNPGAPATWNWAPYDSVYAGALAIGVRPVWT
                     LTNAPCWAAPSPCGPNANPVAGAHVADYAFAAAQIALRYPQSAAIEVWSQPNDAQFWG
                     ARPDPRAFSELVGAAAAAIHATGTGVGVYSGGLLPGGGAADRVEMDQFLSQALAAGGI
                     EEADAIGFHAVADVPFKPGNDPTKGYLGRMRINIQVLDSELADANVTRPIAITGLAFS
                     TAGPGRYTDDQQAEALVSSYEVLRRIGDVPIVLVSRLLDNGDGSKAQGSGVVRSDGSK
                     KPAYCELAKARGAPTPPGCA"
     gene            complement(3645..5063)
                     /locus_tag="VFB52_00675"
     CDS             complement(3645..5063)
                     /locus_tag="VFB52_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04876.1"
                     /translation="MIWAETGTIRGVTAVIAVGLAAGLATPARASAAQNPDHAKAAGI
                     AKLEKKAKQKTKKAVRKNLPGAGTPGLSDPSSPPKIKVGSCARNPKRGRPTAFKCRWK
                     VSGELSGRVPVRCTGKATYKVRKKKVTKVSPCKNREERQAPLLAAPHDVLFGYFEDFT
                     TQGNLFGELRASGSDTLLEGIAWRVLQPNPGGGPATWNWALFDAVYAQALAVGVRPVW
                     TFWDAPCWAAPSPCNPNQVNPVAASRVGDYADAAAQIALRYPQSAGIEIWVEPNSAKF
                     WGAPADPQAFSKLVGDAAAAIHATGTGVGVYSGGLSPGRAAADQHQMDQFLSQALAAG
                     GVERADAIGFHAVTDVPFKPGHDPTKGYLGRMRVNVESLNAEFADANVKLPIAITQLS
                     YSTAGPRAYTDDQQAEALVSSYEVLRRIADIPIIVVSRLLDNGDGSKVQGFGVVRSNG
                     SKKPAYCELAAARGAPTPPGCA"
     gene            complement(5174..6022)
                     /locus_tag="VFB52_00680"
     CDS             complement(5174..6022)
                     /locus_tag="VFB52_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZO04877.1"
                     /translation="MTQADVQLFDPQTLYRRWEDSQWNPFEIDLRRDKEQWAGLARED
                     QDLIHWVLAQLMVAEERITTKFSGLVGAYGSAEEATFLSTQQVDEARHMQFYARFQDE
                     VIAEPATVSAHVERARQEVTPAFRKIFDEALVQAHEKLVAAPQDLAAKVRFVTIYHVI
                     LEATLGLTTFRFATEYLNRQELLPGFVEGYSHIHHDETRHIGYGVWFLRESVRQREDA
                     ADAVSETLRDLLPSVAEALAGAGNGSGPDVERLGVSTDDVRTFALDGLRRRLDIIGVP
                     LTSLAG"
     gene            complement(6019..>6382)
                     /locus_tag="VFB52_00685"
     CDS             complement(6019..>6382)
                     /locus_tag="VFB52_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO04878.1"
                     /translation="VEFLKGTIEEVPLPADSVDVVISNCVISLSADKPAVFGEAARVL
                     RPGGRFAVTDVVADPDMEEETRLDMDNWTGCIAGALTRDEFERKLTAAGFEQIEVRDT
                     HRVHEHAASAIIRARTPA"
BASE COUNT          905 a         2210 c         2243 g         1024 t
ORIGIN      
        1 gctcgaggat ggcctgatcg tccgcatgga cgttcttgaa ccgccggacc agcagtagag
       61 ggtccgaccg caccctgcgc caatggggag cgagaagacc gttcgtcgac actgccgggt
      121 cggcctcgac cggggcccgg ccgcaatgag cctgatcgtc gccggccgag accaggcagt
      181 ccctgctcgt taggtggtgg gctggtcgag gacctcgatg tcaagcgtca tgcccgagga
      241 cttctagtcg cggatcgagt cgagcgcctg cctggcgatc acggtagccg cggagcgcga
      301 ggtccgggga tcggcgcccg gcacgttgaa gttgaccttg accagggtgc gctcgtcgat
      361 cgccgtcagc tgcgagcgat acggcgtcca gtaggcgccg acgccgccgt gggtgcggtc
      421 gtttccgacc ccacggacgc cgaccggttt cgtgctgtcg gggatgtgct cgaaggtcgc
      481 gcgctgcagc ccctcggcga tcaggttgta gtagcgcagc gcggtcttcg gcgcggtgtc
      541 gagggtgacg ctgacgtaga gatcgggccc gcggcgctcg cggtactcgc agatcgagag
      601 gtcgagcgag tcgttggccg agcgctccag cggccgcgcc ggtccgcccg ccgcggcgac
      661 ctcgccttcg atccgccgtt tcgacaccgc gcggcaggcg ggcgacgcgc tcgggtcctc
      721 gcccccgtcg gcctgcgccc tcgtcgtggt cgacgtgctc gtcgcgggcg cgggcgactc
      781 gtcatcgccg cagccgatca tcaccgcggc ggcggtcacg atcgccaggg tcgcgcacca
      841 gatcctcacg aatagcgttc agctaccccg tacggggcgg taccagacgc ccactggcgc
      901 ggcgtctagt cggccgcagg ctcgggcggg cccgcgtcgc ttcgagtgct gacgcgggtg
      961 gacttacgcg aagcgccgct cacaacgcag acaatgagga acgcccttcg gcgtctcgtc
     1021 gcagtccacg cgcgggtagg ctcggcgcat ggctaaccct ggtgagctcg atgcgagctt
     1081 cgccggcgac gggaagaagg cgatcgactt cggcggcacc gacgcggcgc gcgtcgtgca
     1141 ggtccaaccc aacgggagga tcattgtcgc cggcggcggc gcggccgcgg tctccttctg
     1201 cgtcgcgggc ctgcgcacca acggcgcgcc cgataccacg ttcggatcgg gcggaaagcg
     1261 cgtgatcgac ttcggcggtg acgtcgagtc cgtcgagggt gcggcgttgc agcccgacgg
     1321 caagatcgtg ctcgcgggag gctcggacgt gcgggtcgcg gtcgcgcgcc tgaacccgaa
     1381 tggctcgctc gataccgggt tctccggcga cggcaagaag accttcagct ggggcgcgtt
     1441 cagccgggcc tcggccgtgc gcgtgcggcc cgacggcaag atcgtgctcg cgggcttttc
     1501 ggggcccgag ggcgggaaca tcgaggccgc acgcctgaac gcgaacggca agctggacgc
     1561 gacgttcggc accggcggca aggcctcggt tgacttcggc ggcgacgact tcggcctcgc
     1621 gatggcgcgc caagccgacg ggcgcatcct catcgccggc cggtcgtcgg ctgccggcgc
     1681 cgtggtcgcg cggctacggg ctaacggcac gctcgacgcg gacttcgacg gcgacggccg
     1741 cgtgaccctg ccgggcggtg gcagcgcgag cgccgtgctc gtgcagccgg acggcaggat
     1801 cgtcgtcgcg ggcaactcga acggaaacga ggtgatgacc gtcacgcgcc tgcagccgaa
     1861 cgggtcgccc gacgcgacgt ttgacgggga cggcacggcg acaatcgact tcgggtcgct
     1921 ctccgacctc gccagcgacg cggtgctgca ggcggacggc aagatcgtcg tcgccggcta
     1981 cacgcaggcc gacgaggacg tcgcggtcgc gcgcctcaac cccgacggat ctccggacgc
     2041 caccttcggc gccgccggga aggcgaccgt cgacttcggc gtcgccacgt tcggcaacgc
     2101 ggtcgcgctc cagccgaacg gccggatcgt cgtcgccggt cagcggacgg gcggcgacga
     2161 cttcgcagta gcgcgcctgc tcggctgagt cggacgccgg tgcgcgggtg gccggaggac
     2221 tggacgccta cgcgcaacct ggcggggttg gggcgccgcg cgcctttgcg agctcgcagt
     2281 acgccggctt cttcgagccg tcgcttctca cgacgccgga cccctgtgcc ttcgacccgt
     2341 ccccgttgtc aagcaaccga ctgaccagga cgatgggaac atccccgatc cggcgcagga
     2401 cctcgtagct ggagaccagc gcctcggcct gctggtcgtc ggtgtagcga cccgggccgg
     2461 cggtgctgaa ggcgagcccg gtgatcgcga tcggccgggt cacattcgcg tcggcgagct
     2521 cagagtcgag cacctgaata ttgattcgca tacggcccag gtagcccttg gtggggtcgt
     2581 tgccgggctt gaacggcacg tcggcgacgg cgtgaaagcc gatcgcgtcc gcctcctcga
     2641 ttccgcccgc cgcgagcgcc tgggagagga actggtccat ctcaacccgg tccgcggcac
     2701 ccccgccggg caggagcccg ccggagtaga cgccgacgcc ggtcccggtc gcgtggatgg
     2761 ccgcggcagc tgcgccgacc agctccgaga acgcccgcgg gtctggccga gccccccaga
     2821 actgggcgtc gttgggttgg gaccagacct cgatcgccgc ggattgtggg tagcggagcg
     2881 cgatctgcgc tgcagcgaac gcatagtccg cgacgtgagc cccggcaact ggattcgcgt
     2941 tcgggccaca cgggctcggc gccgcccagc agggggcgtt cgtgagagtc cacaccggcc
     3001 ggactccgat tgcaagcgct ccggcgtaga cggaatcgta gggcgcccag ttccaggtcg
     3061 ccggagcacc ggggtttggc tcgagcacat tccaggcgat cccctcgcgg agcgtgttgg
     3121 cgccgctcgc tcccagctcg ccaaagaggt tgccgtgggt cgtgaagtcc tcgaagtagc
     3181 cgaagaggac gtcgtgcggc gcggccagga gcggggtctg gcgcgcctcg cggttctcgc
     3241 acgggcttac cttggtgacc ttcttcttgc ggaccttgta ggtcgccttg cccgtgcacc
     3301 tgaccggcac ccgcccgctc agctcgccgc tgaccttcca cctgcaggta aagcgcgtca
     3361 gccggccgcc gttgctgcgc tccgcacagt tgctgacctc gacccttgga ggcgaggggg
     3421 gtttgctcag gcccggtgtg cgggcgcccg ggaggctctt cttgaccgcc ttcttggtct
     3481 tttgcttggc ctgcttcttg agcttcgcga tccccgctgc cttcgcgtga tccagctcct
     3541 gcgccgccga gccgcgggcg ggcgtcgcga gtccggccac cagcccgacg gcgatcaaag
     3601 cggtgacgcc gcgaatcgtc gctttcccgg tcgggatcac tgtgctacgc gcaacctggc
     3661 ggggtcgggg caccgcgcgc ggcggcgagc tcgcagtacg ccggcttctt cgagccgttg
     3721 cttctcacga cgccgaaccc ctgcaccttc gacccgtcgc cgttgtcgag caaccggctg
     3781 accacgatga tcgggatgtc cgcgatccgg cgcaggacct cgtagctgga gacgagcgcc
     3841 tcggcctgct ggtcgtccgt gtaggcgcgc gggcccgcgg tgctgtagga gagctgggtg
     3901 atcgcgatcg gcagctttac attggcgtcg gcgaactcgg cgttaagcga ctccacgttg
     3961 actcgcatac ggcccaggta gcccttggtg gggtcgtggc cgggcttgaa cggcacgtcg
     4021 gtgacggcgt gaaagccgat cgcgtcggct ctctcgaccc cgcccgcggc gagcgcctgg
     4081 gagaggaact ggtccatctg gtgctgatcg gcggccgccc ggccgggcga gagcccgccg
     4141 gagtagacgc ctacgccggt cccggtcgcg tggatcgcgg cggccgcgtc gcccaccagc
     4201 ttcgagaagg cctgtgggtc cgccggtgcc ccccagaact tggcgctgtt gggctcgacc
     4261 cagatctcga tcccggccga ttgcggatag cggagcgcga tctgtgccgc cgcgtccgcg
     4321 tagtcgccga ccctcgacgc ggcaaccggg ttcacctggt tcgggttaca ggggctcggc
     4381 gccgcccagc agggggcgtc ccagaacgtc cacactggcc gcacgccgac cgccagcgcc
     4441 tgcgcgtaga cggcgtcaaa gagggcccag ttccaggtcg cggggccccc gcccgggttg
     4501 ggctggagca ccctccacgc gatcccctca agcagcgtgt cggagccgct cgctctcagc
     4561 tcgccaaaga ggttgccctg ggtcgtgaag tcctcgaagt agccgaagag gacgtcgtgc
     4621 ggcgccgcca ggagcggggc ctggcgctcc tcgcggttct tgcacgggct caccttggtg
     4681 accttcttct tgcggacctt gtaggtcgcc ttgcccgtgc accggaccgg cacccgcccg
     4741 ctcagctcgc cgctcacctt ccagcggcac ttgaaggcgg tcggccggcc gcgcttgggg
     4801 tttctcgcac agctaccgac cttgatcttc gggggcgagg acgggtcgct cagtcccggc
     4861 gtgccagcgc ccggaaggtt cttcctgacc gccttcttgg tcttttgctt ggcctttttc
     4921 tcgagcttcg cgattcccgc tgccttcgcg tgatccggat tctgcgccgc cgaggcgcgg
     4981 gcgggcgtcg cgagtccggc cgccagcccg acggcgatca cagcggtgac gccgcgaatc
     5041 gtccctgtct cggcccagat cactcccgcg aacgatcatc gctccaagcg gcgctgctgt
     5101 caacaacctc gtggggccgc cacccaccgg actcagccgc gacgaacgcc ttggcgctcg
     5161 cggcgaaacc ggtctaaccc gccaagctgg taagcggcac gccgatgatg tcgaggcgtc
     5221 gcctcagccc gtcgagcgcg aacgtgcgca cgtcgtccgt gctgaccccg agccgctcga
     5281 cgtctggccc cgacccgttg ccggcgccgg ccaaagcttc ggcgaccgac gggagaagat
     5341 cacgcagcgt ctcgctgacc gcgtcagccg catcctcgcg ctgccggacg ctctcccgca
     5401 ggaaccagac cccgtacccg atgtgtcggg tctcgtcgtg gtggatgtgg gaatagccct
     5461 caacgaaacc gggcagtagc tcctggcggt tcaggtactc ggtggcgaac cggaacgtgg
     5521 tcaggccaag cgtggcctcg agtatcacgt ggtagatggt cacgaagcgg accttcgcgg
     5581 ccaggtcctg aggagcggcg accagcttct cgtgggcctg gaccaaggct tcatcgaaga
     5641 tcttgcggaa ggcgggggtc acctcctggc gagcccgctc gacatgcgcg ctgacggtgg
     5701 caggctcggc tatgacctcg tcctggaagc gcgcgtagaa ctgcatgtgc cgcgcctcgt
     5761 cgacctgctg cgtcgagaga aacgtcgcct cctccgctga gccgtacgcg cccaccaatc
     5821 ccgagaactt ggtggtgatc cgctcctcgg cgaccatcaa ctgagccagg acccagtgga
     5881 tgaggtcctg gtcctcccgg gccagcccgg cccattgctc cttgtcgcgc cggagatcga
     5941 tctcgaatgg gttccactgc gagtcctccc accgccgata gagcgtctgg gggtcgaaga
     6001 gctggacgtc ggcctgggtc acgccggcgt ccgagcgcgg atgatcgccg aggcggcgtg
     6061 ctcgtggacg cgatgggtgt cgcgaacctc gatctgctcg aagccggcgg cggtcagctt
     6121 gcgctcgaac tcatcccgag tcagggcgcc ggcgatgcac ccggtccagt tgtccatatc
     6181 gagtctcgtc tcctcctcca tgtcgggatc ggcgaccacg tcggtgaccg cgaagcggcc
     6241 accggggcga agcacccggg cggcctcgcc gaaaaccgcc ggcttatcgg cggacaggct
     6301 gatcacgcag ttggagatca ccacatcgac ggagtcggcg ggcagcggca cctcctcgat
     6361 cgtgcccttc aggaactcga cg
//
LOCUS       DASRIG010000031         4833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3405491, whole genome shotgun sequence.
ACCESSION   DASRIG010000031 DASRIG010000000
VERSION     DASRIG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405491"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VFB52_00690"
     CDS             <1..1588
                     /locus_tag="VFB52_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO04879.1"
                     /translation="LRPEKVVGFHFFYPASVMPLVEIVEGDETDPETVQAAYNFAQAI
                     RKQPITCAEVPGFVVNRILNSAVGEIWRAQAEQGLSIKQIDEAITAANVAPMGPFFLG
                     DLLGLDTILHVAEHLRDAYGDSFYVHEGMRKLVADGHLGAKSGGDGFYKDGEPQLEGD
                     AEPDGEALAELLKLKALVEACLLLEEGVCTVRDIDLGMMAGAGMDPRRGIFPPFMGAD
                     LAGLDDTLEKLERAQNEHGERFAPPKILKRLVAQGRHGQKSGQGFYAYPRPDEGEQAD
                     AVKLETRGDVAIAWLVNPPMNAISPQLMKDLETVWERVKASDEIRALVIASSNPVVFS
                     AGADIKAFASMDEAGGRELIHGGHALLRELGLNRVSTVAAVNSLAFGGGCELAMACDF
                     RIAAESAVFGQPEIKLGIIPGFGGTQRLPRLVGAAKALEMNLIGDAISSGEAEAYGLA
                     NRVVPDHELFDTAVAWARKLAAQAPIAVQEVKRASDDPDLDEGIEKEKKSFERAFQSE
                     DGREGISAFLGKRTARWQGK"
     gene            1585..2352
                     /locus_tag="VFB52_00695"
     CDS             1585..2352
                     /locus_tag="VFB52_00695"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZO04880.1"
                     /translation="MSSRTDSRPHCPAAELAALIRDSESVVALTGAGISVPSGIPDFR
                     TPGKGLWAKVDPMEVAHIDAFHRDPRRFWGFYRPRFADLDEKRPNGAHEALAELEARG
                     ILAAVITQNIDRLHGKAGSDRVVEVHGSIATSSCTSCRASYPLESVGELFGADGVATC
                     ACCTGKVKPDVVLFGEVLPEAAMAEAHELCASADLLLCVGSSLEVYPVASLPEVTLDG
                     GGRVAIVTQGATPYDREAVVKLDGDVVTELEAVLAAL"
     gene            complement(2512..3363)
                     /locus_tag="VFB52_00700"
     CDS             complement(2512..3363)
                     /locus_tag="VFB52_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04881.1"
                     /translation="MSGQDRLPFVQFELAGTVGLEQGRYLGSDPERVLVVTVAGAPMP
                     PRRRLGRVKPKDADPDADPPTVPLTTLTVVRPDPLGDEPGAERWLGELRDEPDATAEE
                     VRDAVVFVNGALHAHRAAVLDPDLADVTAPHALAVRIGFGSGDELADGRFTRAFEVPR
                     SARRRRGEVLRPQERVAGVLGGRETVAACELLVLRARSDLDAGRGREAALQLRVGLEA
                     LLAEREVLAAPGQDEDLAVLAERRSITGQAANEALGGELGDGRMAEVTETLRLCERVL
                     RRRRALG"
     gene            complement(3400..>4833)
                     /locus_tag="VFB52_00705"
     CDS             complement(3400..>4833)
                     /locus_tag="VFB52_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO04882.1"
                     /translation="PGKRAYGNAAEVISSSPTRRGIDIYSREGAPVVAVNDGVITEIG
                     NSKKLGRFIVLRDAYGNRFTYAKLGRVSDVYPVQKQQRLSAADFELVSPSDPKPKQAA
                     RAGNNDVARRAERKAQPADGPTNTEDTRERLYAFPERASNVDRADLTGQLDSLMKDRF
                     PGYESFKSYFSDVLRFDSKTMDMRQLKEGSHVIAGTVLGKIGKVDEVAPRVHFAIRPA
                     GRGAPKIDPKPILDGWKLLEATAIYRAAGENPLADGATTGQVLLMSKPQLIRRVLADP
                     RLEIYACGREDIRSGQVDRRVLALLEYLVERGFRLTVSSLKCGHSVYTSSGSISHHSS
                     GNAVDIPQINGIPVLGNQGPGSITESLVKEVMALQGNMRPAQIISLMDFGADNTFAMS
                     DHADHVHVGFQPPFGPGSGSVSRQFTEILKPDQWERLIDRIGKIDNPSVPTSPSRYAA
                     PAERGHDEAAEDGAGKRRRASSAHLGE"
BASE COUNT          695 a         1690 c         1720 g          728 t
ORIGIN      
        1 cctgcggccc gagaaggtcg tcggcttcca cttcttctat cccgcctcgg tgatgccgct
       61 ggtcgagatc gtcgagggcg acgagaccga ccccgagacc gtccaggccg cctacaattt
      121 cgcccaggcg atccgcaagc agccgatcac gtgcgccgag gtaccgggct tcgtcgtaaa
      181 ccggatcctc aacagcgccg tcggcgagat ctggcgcgcc caggcggagc agggcctctc
      241 gatcaagcag atcgacgagg ccatcacggc cgccaacgtg gcgccgatgg gtcccttctt
      301 cctgggcgac ctgctcggcc tcgacacgat cctccacgtc gccgagcacc tccgcgacgc
      361 atacggcgac tcgttctacg ttcacgaggg gatgcgcaag ctggtcgcgg acgggcacct
      421 cggcgcgaag tccggcggtg acggcttcta caaggacggc gagccgcagc tcgagggcga
      481 cgccgagccc gacggcgagg cgctcgccga gctgctgaag ctgaaggcgc tggtcgaggc
      541 gtgcctgctg ttggaggagg gcgtgtgcac cgtccgcgac atcgatctcg ggatgatggc
      601 cggagccggc atggacccgc ggcgcgggat cttcccgccg ttcatgggcg ccgacctcgc
      661 cggtctcgac gacaccctcg agaagctcga gcgggcacag aacgagcacg gcgagcgctt
      721 cgcgccgccc aaaatcctga agcggctcgt cgcccagggg cgtcacggcc agaagtccgg
      781 tcagggcttc tatgcctacc cgcggcccga cgagggcgag caggccgacg cggtgaagct
      841 cgagactcgc ggcgacgtcg cgatcgcctg gctcgtcaac ccgccgatga acgcgatctc
      901 gccccagctg atgaaggacc ttgagacggt ctgggagcgg gtgaaggcct cggacgaaat
      961 ccgggcgctg gtcatcgcct cctcgaaccc ggtcgtgttc tcagccggcg ccgacatcaa
     1021 ggcgttcgca tccatggatg aggccggcgg gcgcgagctc atccacggcg ggcacgcgct
     1081 gcttcgcgag ctgggcctga accgcgtctc gaccgtcgcc gcggtgaact cgctcgcctt
     1141 cggcggcggc tgcgagctgg cgatggcctg cgacttccgg atcgcggccg agtcggccgt
     1201 cttcggccag cccgagatca agctcgggat catccccggc ttcggcggca cccagcgcct
     1261 gccgaggctg gtcggcgcgg cgaaggcgct ggagatgaac ctgatcggcg acgcgatctc
     1321 ctccggcgag gcggaggcat acgggctcgc aaatcgcgtc gtccccgacc acgagctctt
     1381 cgacactgcg gtcgcctggg cccgcaagct cgccgcccag gcgccgatcg ccgtccagga
     1441 ggtcaagcgc gcctcggacg atccggacct cgacgagggg atcgagaagg agaagaagag
     1501 cttcgagcgg gccttccaat cggaggacgg ccgggagggg atcagcgcgt tcctcggcaa
     1561 gcggacggcg aggtggcagg ggaagtgagc tcgcggaccg actccagacc gcactgtccg
     1621 gcggccgagc tggccgcgct gatccgcgac tccgagtcgg tggtggcgct gaccggcgcg
     1681 ggcatctcgg tgccgtccgg gatcccggac ttcaggaccc ccggcaaggg gttgtgggcg
     1741 aaggtggatc cgatggaggt cgcccacatc gacgccttcc atcgcgaccc caggcgtttc
     1801 tggggcttct accggccccg attcgccgac ctggacgaaa agcggcccaa cggcgcccat
     1861 gaagctctag ctgagctcga ggctcgcggg atcctggccg cggtgatcac acagaacatc
     1921 gaccgcctgc acggcaaggc gggctccgat cgcgtggtcg aggtgcacgg ctcgatcgcc
     1981 acctcgagct gcacgagctg tcgcgccagc tatccgctcg agagcgtcgg cgagctgttc
     2041 ggcgcggacg gggtcgcaac gtgcgcctgc tgcacaggca aggtcaagcc cgacgtcgtg
     2101 ctcttcggcg aggtcctgcc cgaggcggcg atggccgagg cccacgagct ctgcgcgagc
     2161 gccgacctgc tgctctgcgt cggctcctcg ctcgaggtct acccggtcgc ctcgctcccg
     2221 gaggtcaccc tcgacggcgg cggcagagtc gcgatcgtca cccagggcgc gacgccgtac
     2281 gaccgcgagg cagtggtgaa gctcgacggc gacgtggtga ccgagctcga ggcggtgctg
     2341 gcggcgcttt agcgcatgat ctcgcgtcca ttgtcgtgtt aagcaccgta tgcgtgccta
     2401 acacgacaat cgatgccgtg gaggggggcg ccgcgcctgc tggcctcccc tacctgaccc
     2461 agcccggcct cgtccaccgc cgccgcccga gcggaacgcc gccggtcgcc ctcaccccag
     2521 cgcccgccgg cgtctcagca cgcgctcgca cagccgcaac gtctcggtca cctcggccat
     2581 acgtccgtcg ccgagctcgc cgccgagcgc ctcgttcgcg gcttgaccgg tgatcgagcg
     2641 ccgctcggcg aggacggcga gatcctcgtc ctgcccgggg gctgcgagca cctcgcgctc
     2701 ggcgagcagc gcctcgagcc cgacgcgcag ctgcagcgcc gcttcccggc cgcggccggc
     2761 gtccagatcc gagcgcgccc gcagcaccag cagctcgcat gccgcgaccg tctcgcggcc
     2821 cccgagcacc ccggcgaccc gttcctgcgg gcgcagcacc tcgccgcgac gacggcgcgc
     2881 cgagcgcggg acctcgaacg cccgcgtgaa tctgccgtcc gccagctcgt cgccgcttcc
     2941 gaacccgatc cgcaccgcca gcgcatgcgg ggccgtgaca tcggcgaggt ccggatcgag
     3001 caccgctgcg cggtgcgcgt gaagcgcgcc gttgacgaac acgaccgcgt cacgcacctc
     3061 ttcggccgtc gcgtcgggct cgtcgcgcag ctcgccgagc cagcgctcgg caccgggctc
     3121 gtcgccgagc ggatcggggc gcaccacggt cagggtcgtc agcgggaccg tgggggggtc
     3181 ggcgtcgggg tcggcgtcct tgggtttcac ccggccgaga cggcggcgcg gcggcatcgg
     3241 ggcgcctgcg acggtcacga cgagcacccg ctccgggtcg ctcccgaggt agcggccctg
     3301 ctcgagcccg accgtaccgg cgagctcgaa ctggacgaag ggcaggcgat cctggccgct
     3361 cacggcgcgg acgctattcg acggcgccgc tgcggccggt tactcgccca ggtgggcgct
     3421 ggacgctcgg cggcgcttgc cggccccgtc ctctgccgcc tcgtcgtgac ccctctccgc
     3481 cggggccgcg taacgggagg gcgaggtcgg cacgctgggg ttgtcgatct tgccgatccg
     3541 gtcgatcagg cgctcccact ggtcgggctt gaggatctcc gtgaactgcc gcgagacgct
     3601 gccggagccc gggccgaacg gaggctggaa gccgacgtgc acgtgatcgg cgtggtcgct
     3661 catggcgaag gtgttgtccg cgccgaagtc catgagcgag atgatctggg cgggccgcat
     3721 gttgccctgc agcgccatca cctccttgac cagcgactcg gtgatcgatc cgggcccctg
     3781 attgccgagc accgggatgc cgttgatctg cgggatgtcg accgcgttcc cggacgagtg
     3841 atggctgatg ctgccggagg aggtgtagac ggagtgaccg cacttgagtg agctgacggt
     3901 caggcggaag ccgcgctcga cgaggtactc gagcagcgcc agcacgcggc ggtcgacctg
     3961 accgctcctg atgtcctcgc gcccgcaggc atagatctcg agccgcggat cggcgagcac
     4021 gcgcctgatc agctgcggct tcgacatcag cagcacctgg ccggtggtgg cgccgtcggc
     4081 gagcgggttc tctccggcgg cgcggtagat cgccgtggcc tcgagcagct tccagccgtc
     4141 gaggatcggc tttgggtcga tcttcggcgc gccgcgaccg gcggggcgga tcgcgaagtg
     4201 gacccgcggc gcgacctcgt cgaccttgcc gatcttgccg agcacggtcc cggcgatcac
     4261 gtgcgagccc tccttcagct gccgcatgtc catcgtcttg gagtcgaacc gaagcacgtc
     4321 ggagaagtac gacttgaacg actcgtagcc ggggaaccgg tccttcatca gggagtcgag
     4381 ctggcccgtg agatcggcgc ggtcgacgtt gctcgcccgc tcgggaaacg cgtagaggcg
     4441 ctcgcgggtg tcctcggtgt tcgtcggtcc gtcggccggc tgcgccttgc gctcggcgcg
     4501 tcgcgccaca tcgttgttgc ccgctctcgc tgcctgcttc ggcttcgggt cgctcgggga
     4561 gacgagctcg aagtcggcgg ccgagaggcg ctgctgtttc tgcaccgggt agacgtcgga
     4621 gaccctgccc agcttcgcgt aggtgaaccg gttgccgtag gcgtcgcgca ggacgatgaa
     4681 gcggccgagc ttcttggagt tgccgatctc ggtgatcacg ccgtcgttga cggcgaccac
     4741 cggggcgccc tcgcgcgagt agatgtcgat ccctcggcgt gtgggcgagg aggagatcac
     4801 ctcggcggcg ttgccgtagg cgcgcttgcc ggg
//
LOCUS       DASRIG010000032         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2361253, whole genome shotgun sequence.
ACCESSION   DASRIG010000032 DASRIG010000000
VERSION     DASRIG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361253"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFB52_00710"
     CDS             <1..615
                     /locus_tag="VFB52_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO04883.1"
                     /translation="IVGHSLGGGVALQFTYQFPEMVERLVLVSSGGLGPDVSPILRAA
                     ALPGADLFISATAGIGQRAGSLLGSGLGRLGLQPNADVAEVARGYATLSDRDRRRAFL
                     ATLRSVVGTEGQLVGATDKLYLAAELPLLILWGDRDPIIPVSHAEEAHAALPASRLEI
                     FEDTGHVPQLERPGRFVAALQRFLDETEPAHFDRDEWRARFKTA"
     gene            complement(670..743)
                     /locus_tag="VFB52_00715"
     tRNA            complement(670..743)
                     /locus_tag="VFB52_00715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Arg,seq:ccg)
     gene            822..1790
                     /gene="rnz"
                     /locus_tag="VFB52_00720"
     CDS             822..1790
                     /gene="rnz"
                     /locus_tag="VFB52_00720"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZO04884.1"
                     /translation="MDLSVVFLGTGGSVPSARRSTASVLIARGGDRMLFDCGEGTQRQ
                     LQRSLGLVQLDEIYLTHFHADHILGLPGLLKTYDLTDREAPLRIYGPSGLRELFKVLQ
                     PLIGRLGFALDLEELEPGDVVDRDGYEIQSFEAAHSVRASGYALVEHERPGRFDPAAA
                     KAAGVPEGPAFAALQRGEEVEGSEGTVSPRQVMGESRPGRTIALTGDTAPSPATVAAA
                     ADAELLIHDSSFAEEEAQRAAETGHSTVGQAAAVAAEAHVKLLALVHISSRYHVGTVL
                     DEAREVFEPTVAPRDFDVIEIPFPEKGEPQLVPNGARERSVQAEPA"
     gene            1841..>2106
                     /locus_tag="VFB52_00725"
     CDS             1841..>2106
                     /locus_tag="VFB52_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZO04885.1"
                     /translation="MPLSPTTSSPPSIAAEVKRCCEGDPGATEALARHATRLALRTGA
                     ALLESREEAGDVAQDVAIDVLRSLHGLRDPEAFDGWVHRITVRHA"
BASE COUNT          306 a          792 c          681 g          327 t
ORIGIN      
        1 atcgtcggcc actcgctcgg cggcggtgtc gccctccagt tcacctacca gttcccggag
       61 atggtcgagc ggctggtgct ggtctcaagc ggcggcctcg gtcccgatgt cagcccgatc
      121 ctgcgggccg cggcactgcc cggcgccgac ctcttcatct ccgccaccgc cgggatcggc
      181 cagcgcgcgg gctcgttgct tggctccggc ctcggccggc tcggcctgca gccgaacgcc
      241 gacgtcgccg aggtcgcgcg cggctacgcg accctctccg accgcgatcg ccgcagggcc
      301 ttcctcgcca ccctgcgctc ggtcgtcggc accgagggtc agttggtcgg cgccaccgac
      361 aagctctacc tggcggccga gctgccgctg ctgatcctct ggggtgaccg cgacccgatc
      421 atccccgtct cccacgctga agaagcccac gccgcgctcc ccgccagccg cctggagatc
      481 ttcgaggaca ccggccacgt cccccagctc gagcgccccg gccgcttcgt cgccgccctg
      541 cagcgcttcc tcgacgaaac cgagcccgcc cacttcgacc gcgacgagtg gcgcgcgcgg
      601 ttcaagaccg cctgatcgac gagtctgctc tggcccccgt atagcggggt caaacgcaga
      661 ctcgcgggac gcgcccggca ggattcgaac ctgcggcctc ttcctccgga gggaagcgct
      721 ctatccagct gagctacggg cgctcggccg ccagcttagc cagtctgact ccacgactcg
      781 ccccgcaagc cccccgaagt aggccgtacg cttcgccgcc catggatctg tcggtggtct
      841 ttctcggtac tggtggctcg gtgccttcag cacggcgttc gacggcgagc gtgctgattg
      901 cgcgtggggg ggaccggatg ctgttcgact gcggggaagg gacgcagcgg cagctgcagc
      961 gctccctcgg gctggtccag ctcgacgaga tctacctcac ccacttccac gctgaccaca
     1021 tcctcgggct gccgggcctg ctgaagacct atgacctcac cgatcgcgaa gcgcccctgc
     1081 ggatctacgg ccccagtggg ctgagggagc tgttcaaggt gttgcagccg ctgatcggcc
     1141 ggctcggttt cgcactcgac ctggaggaac tggagcccgg cgacgtcgtc gaccgcgacg
     1201 gctacgagat ccagtccttt gaagccgccc acagtgtgcg ggcgagtggc tacgcgctgg
     1261 tcgagcacga gcggccgggc cgcttcgacc cggctgcggc caaggccgcc ggcgtgcccg
     1321 aagggccggc gttcgcggcg ctgcagcgcg gggaggaggt cgagggctcc gagggcacgg
     1381 tctcaccgcg ccaggtgatg ggggagagcc gcccgggacg cacgatcgcc ttgactggtg
     1441 acaccgctcc cagtcccgcc acggtcgccg ccgccgccga cgccgagctg ctgatccacg
     1501 actccagctt cgccgaggag gaggcccagc gcgccgccga aaccggccac tcgaccgtcg
     1561 gccaggccgc cgcggtcgcc gccgaggccc acgtaaagct cctcgctctc gtccacatct
     1621 cctcccgcta ccacgtcgga accgtcctcg acgaggcccg cgaagtcttc gaacccaccg
     1681 tcgccccgag agatttcgac gtcatcgaaa tcccattccc cgagaaaggt gaaccccagc
     1741 tcgtcccgaa cggagcccgg gaacgcagcg tccaggccga gcccgcctga ctttcgtcgg
     1801 cgttgcaagg tttttcgggt tccctgcccc taggtcggta gtgccactct ctccgaccac
     1861 cagctcccca ccctcgatcg ctgccgaagt gaaacgttgt tgcgaaggcg acccgggcgc
     1921 gaccgaggca ttggcacgcc acgcgacgcg cctcgcgcta cgcaccggtg cggccctgct
     1981 ggaaagccgc gaggaggccg gcgacgttgc ccaagatgtg gcgatcgacg ttctgcgctc
     2041 gctgcacggg ctgcgtgacc cagaggcctt cgatggatgg gtacatcgga tcaccgtgcg
     2101 tcacgc
//
LOCUS       DASRIG010000033        10839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3140085, whole genome shotgun sequence.
ACCESSION   DASRIG010000033 DASRIG010000000
VERSION     DASRIG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140085"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFB52_00730"
     CDS             <1..506
                     /locus_tag="VFB52_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZO04886.1"
                     /translation="VELAAFEIDRTPVTNAAYTEFMEATGAEPPEYWQPDGDGWVDTS
                     RGRREPIAPDYPVIHVSWDQADAFARWAGKRLPTEFEWEAARERLADVGQAWEWTSSD
                     FLAYPGFAPFPYSEYSEAFFGDTYKVLRGGSWATHARVARPTFRNWDLPERRQIFSGI
                     RCARDAS"
     gene            503..1474
                     /gene="egtD"
                     /locus_tag="VFB52_00735"
     CDS             503..1474
                     /gene="egtD"
                     /locus_tag="VFB52_00735"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZO04887.1"
                     /translation="MIAIDVHLDADAHAAMVEDVRTGLTASPKELSPKYFYDERGSQL
                     FEQITELPEYYPTRREREILADRSAEILAAAGAPGTLVELGSGSASKTRHLLTAMREA
                     GCLRTYVPVDISEEITHETALALVEEYPGLDVRGLVCDFEVDLERIPSDGDARLLAFL
                     GGTIGNLYPGERREFLTRIGAMFGPDDHLLLGTDLVKETARLEAAYDDAAGVTAEFNK
                     NVLAVLNRELGADFDLDAFEHVARFDAREERMDIRLRSLTEQSVHLAELDLTVEFAAG
                     EEMRTEISAKFTPERLECVYAESGLELRGWFTDAAGDYALSLARPRA"
     gene            complement(1471..2403)
                     /locus_tag="VFB52_00740"
     CDS             complement(1471..2403)
                     /locus_tag="VFB52_00740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZO04888.1"
                     /translation="MEGRWGLTLPLPGVSLGDHRELLQAAEAAGYTDFWTGETAGPDG
                     FTPLTLSAAWTSRVRLGTGIVGVFQRGPALLAQEAAALADASNGRFVLGIGSSSDRIV
                     EGWNGIPFERPLSRVRETLDFLDAALTGERTSTGFKLESPPVHRVPVVLAALRGKMLE
                     LAVERAAGAFTNFLPLSGLPKVTAQLDGAPEGFELLCRFFCIPGEREQAEPLARFMFS
                     SYITVPVYTAFYRWLGFGEQIDPMVTAWQAGDRQAAAAAAPWELIEDMFVFGSPEQMK
                     ERLAAFVAGGITLPIITPIVPPEQLGETIAALAP"
     gene            2433..3440
                     /gene="glpX"
                     /locus_tag="VFB52_00745"
     CDS             2433..3440
                     /gene="glpX"
                     /locus_tag="VFB52_00745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZO04889.1"
                     /translation="MAEASQAKPDRNLALELVRVTEAAALASARWVGRGDKLAADGAA
                     VDAMRFVLDSVSMDGVVVIGEGEKDEAPMLYNGEQIGDGSPPSVDIAVDPLEGTRLCA
                     LGMPSAIAVIALAERGSMFDPGPCVYMEKMAGGEEIADLLDLDRPLPETLKLIADRKG
                     GTIGDVIVVMLDRPRHEDAIREVREAGARIRLITDGDVSAALLAVSERSPVDLLWGIG
                     GTPEGVISAAALKSMGGQLLGRLWPRNDEERAAALDAGYDLDRVLTANDLCSGEDVFF
                     SATGVTDGDVLQGVRYRGAGVTTESLVMRSRSGTVRRVHARHDREKLRAILGESAKRY
                     G"
     gene            3481..4392
                     /locus_tag="VFB52_00750"
     CDS             3481..4392
                     /locus_tag="VFB52_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZO04890.1"
                     /translation="MIAVAGSTGYIGGLLCRQLREAGEEVRALARNPERAGELTEIGC
                     EVCQADVLDPSSLRKALDGADVAYYLVHSMGRGADDGDFAARDRQGAENFAAAAADAG
                     VRRIVYLGGLGGLGKGSEHLASRHATAETLRAGAVPVAYFRAAAVIGAGSESFRTVFY
                     LVRRLPVMVTPRWVVTRTQPIAVDDAVGFLADARDLGLPLDRELQIGGPDVTTYGGMI
                     DELARALDRRPPLRITVPVLTPALSSLWIGLVTPVDAGVARPLIEGLATETVVTDPTG
                     MELLPERERVPLSQALHIAAENVTVRL"
     gene            4507..5478
                     /locus_tag="VFB52_00755"
     CDS             4507..5478
                     /locus_tag="VFB52_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZO04891.1"
                     /translation="MGLERPEADRFRAGEQIFTILATPLNVMVLRALARRPMRLAELR
                     QATGLPAQTTLRGHLTSLAEIGAVSKRPTAQMPYAVENQLTQMGEDLLAVADRLEAWL
                     ALAPEAPISLEAGGARGVVKAFIDGWGSTMMRGLAARPMSLTELDRQIPDLSYPALER
                     RLSSMRMAGLIEAQPGTGGGTPYAVTDWARRGVLPLAAAAACEGTHLGNAAPLVSEID
                     IEAAFMLATPLVGLTPDLAGSCQLEVEAEPDHSRDASGVQVAVEGGRVVACDCELGPS
                     PAGYATGSTAHWFNAILEGTASLLSFGGSRQLSEAMVSGLHSALVTR"
     gene            complement(5444..6142)
                     /locus_tag="VFB52_00760"
     CDS             complement(5444..6142)
                     /locus_tag="VFB52_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO04892.1"
                     /translation="MTNEPEDLRKWRLPRGRHGLPRELVTRSQRERLLAAVVRATAAK
                     GYGATTVADILDEAGVGRETFYELFDDKRTCALTAHALLTDDLEEKVRAAYMEPGEWP
                     ERMRRALARALEWFAADPPAAKFMLVELAGMGTESRVRFQAEFSRFVALLDEGLPDAP
                     AAPDVSQATSLGVGAVLARVYEEIVLGRAASLPELLPDLTFELLVPFEGEEVARVERG
                     RAVASGSQARSEDR"
     gene            complement(6139..6795)
                     /locus_tag="VFB52_00765"
     CDS             complement(6139..6795)
                     /locus_tag="VFB52_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO04893.1"
                     /translation="MSGVLDPKTPTDIAARAQRARIIDAMVASCAEKTFPGTTISDIV
                     GQASISRTTFYKHFTDKRACFEAAVDSGIEEVRTVARNAHTPADPPADAVLAATAAIV
                     RLMAEKPGLAQLLTGDAPAVEPAAVERYRRLLIPAVSGLWEAAGEQGEPRMDPNLAFG
                     RAQLLIFNQIAAGKAASLPDLLPETVYLAVAPFVGHEEGLKQARRAEADANPERSLSR
                     "
     gene            complement(6825..7748)
                     /locus_tag="VFB52_00770"
     CDS             complement(6825..7748)
                     /locus_tag="VFB52_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZO04894.1"
                     /translation="MPSSLRNPRPWLQAFWRRAYEENVTGLSAMVAYNLMLAVFPFAL
                     LVLFVFGQVLKIEGVETSVLSDLQRLFPSVEQDTLTDVLNRIENNSTTIGIAAFVGSL
                     WIGASFWGAMDTAFCRIYHVECRGWIEQKRFSFAMLFVVVLFIAASIFVPAVESALLS
                     STDRLPFGLSSIKAIDTVLLLAAALLVTFGICSVIFWAVPKGHMPWRAVWPGAAFVTF
                     AAGLANWLFPIYLSTVSSLSRFGSTLGFILIALLWFYVLSLALMVGAVINSLRHELHD
                     TGELPYRASARPCQGCDESPQAAADNVDVPR"
     gene            7961..9067
                     /locus_tag="VFB52_00775"
     CDS             7961..9067
                     /locus_tag="VFB52_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO04895.1"
                     /translation="MLVAELQELEEVKNLINRGQQLGVLTFGDVASAVSEVDLDESDV
                     EDLYGHLEKSGIELVEDMDPAQKAAADAMPADTGKKGRRRQKQALDLKPDMTTDSLQL
                     FLKDIGKVRLLTAAEEVELAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLD
                     LIQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLN
                     KIGRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQAPVSLEKPVGDEEESEF
                     GQFIADEKAESPFDRAADLLTKEALKEALENLSYRERRVLELRYGLGGEHPRTLDEVG
                     RTFNVTRERIRQIENQSLKKLQSLGEAQKLREVA"
     gene            9233..10345
                     /locus_tag="VFB52_00780"
     CDS             9233..10345
                     /locus_tag="VFB52_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZO04896.1"
                     /translation="MFDIDSALRTLVEREGSDLHLKVGSPPVARMHGELIPLEGFGDL
                     ADADTEKALHDIAEVRSLTEFEEEGEADFSYALEGVSRFRVNSFKQRGSISIVCRAIP
                     FTIRSIDDLGLPDVVRSLAEEERGIVLVTGTTGSGKSTTLAAMIDHINLNRARHIITL
                     EDPIEYLHPDKRSIVNQREVGFDTKGFGRAMRRVLRQDPDVILIGEMRDEETVRTALS
                     AAETGHLVLSTVHTLDATETINRIIDFFPPHLQQQARVMLAATLKGAIAQRLVPDVTG
                     EGRVPASEVMVVTGRVQDLIMNPEETGKITEVIAEGSYYGMRTFDQSLLGYAMEGRIS
                     ERTAMEFASSPHDFKLMLAAGGQRASGIEQMETAAS"
     gene            10342..>10839
                     /locus_tag="VFB52_00785"
     CDS             10342..>10839
                     /locus_tag="VFB52_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04897.1"
                     /translation="MREKLNNDPKMQVALVAVLVVAALLFFLKSSGGEEEGESGGAVA
                     TVNGETATGSTPGEAVDAAIEGLETGAVEAGASAVPASVPAPPPPPAFTSAYDANKTV
                     VLLVVHDAGIDDRLTAKAADAVEGMAEAELIRVPLKQLPRYAAVTVGLELNRVPALVV
                     VRPRGL"
BASE COUNT         1769 a         3699 c         3784 g         1587 t
ORIGIN      
        1 cggtcgagct ggccgccttc gagatcgacc gaaccccggt caccaacgcc gcgtacaccg
       61 agttcatgga agcgacgggg gccgagccgc ccgagtactg gcagcctgac ggcgacggat
      121 gggtcgacac ctcgcgcggc cgccgcgagc cgatcgctcc cgattacccg gtgatccatg
      181 tcagctggga ccaggccgac gccttcgccc gctgggccgg caagcggctg ccgaccgagt
      241 tcgagtggga ggcggcccgc gagcggctcg ccgacgtcgg ccaggcctgg gaatggacct
      301 cctccgactt cctcgcctac cccggcttcg cgcccttccc ctacagcgag tactccgagg
      361 ccttcttcgg cgacacctac aaggtcctgc gcggcggctc ctgggcgacc cacgcgcggg
      421 tcgcgcgacc gaccttccgc aactgggacc tgcccgagcg gcgccagatc ttctccggca
      481 tccgctgtgc gagggacgcg tcgtgatcgc gatcgacgtc cacctcgacg ccgacgccca
      541 cgcggcgatg gtcgaggacg tgcgcaccgg cctcaccgcc tcgccgaagg agctctcgcc
      601 gaagtacttc tacgacgagc gcggctcgca gctattcgag cagatcaccg agctgcccga
      661 gtactacccg acccgtcgcg agcgcgagat cctcgccgac cgctcggccg agatcctcgc
      721 cgccgccggc gcccccggca ccctggtcga gctcggctcc ggctccgctt cgaagacccg
      781 ccacctgctg accgcgatgc gcgaggcggg ctgcctgcgc acctacgtgc cggtcgacat
      841 ctccgaggag atcacccacg agaccgccct cgccctggtc gaggagtacc cggggctcga
      901 cgtgcgcggc ctcgtctgcg acttcgaggt cgacctcgag cggatcccca gcgacggcga
      961 cgcgcgcctg ctcgccttcc tcggcggcac gatcggcaac ctctacccgg gggagcgccg
     1021 cgagttcctg acccggatcg gggcgatgtt cggacccgac gaccacctcc tgctggggac
     1081 cgacctggtc aaggagacgg cgcgcctgga ggccgcctac gacgacgccg ccggggtgac
     1141 cgccgagttc aacaagaacg tgctggcggt gctcaaccgc gagctcggcg ccgacttcga
     1201 cctcgacgcc ttcgagcacg tcgcccgctt cgacgcccgc gaggagcgga tggacatccg
     1261 cctgcgctcg ctgaccgaac agagcgtcca cctcgccgag ctcgacctca ccgtcgagtt
     1321 cgcggcggga gaggagatgc ggaccgagat ctcggccaag ttcacccccg agcggctcga
     1381 gtgcgtctac gccgaatccg gcctcgagct gcgcggctgg ttcaccgacg ccgctggcga
     1441 ttacgcgctc agcctcgccc gcccgcgcgc ttagggagcc agcgcagcga tcgtctcgcc
     1501 cagctgctcc ggcggcacaa tcggggtgat gatcggcagc gtgatcccgc ccgcgacgaa
     1561 cgcagccagc cgctccttca tctgctcggg cgagccgaag acgaacatgt cctcgatcag
     1621 ctcccagggt gccgccgccg cggccgcctg acggtcgccc gcctgccagg cggtgaccat
     1681 cgggtcgatc tgctcgccga agccgagcca gcggtagaag gcggtgtaga ccgggacggt
     1741 gatgtaggag gagaacatga agcgggccag tggctcggcc tgctcgcgct cacccgggat
     1801 gcagaagaag cggcagagca gctcgaagcc ctcgggggcg ccgtcgagct gggcggtcac
     1861 cttcggcagc ccggagagcg gcaggaagtt ggtgaaggcg ccggccgccc gctcgaccgc
     1921 gagctcgagc atcttgccgc gcagcgccgc gagcacgacc gggacgcggt gcacgggcgg
     1981 cgattccagc ttgaagccgg tcgacgtgcg ttcgccggtc agcgccgcgt cgaggaagtc
     2041 gagcgtctcg cgcacccgcg agagcggccg ctcgaagggg atcccgttcc agccttcgac
     2101 aatccggtcc gaggaggagc cgatcccgag cacgaagcgc ccgttggagg catcggcgag
     2161 cgccgccgcc tcctgggcga gcagcgccgg gccgcgctgg aagacgccga cgatgccggt
     2221 gccgaggcgc acgcggctgg tccaagcggc gctcagggtg agcggggtga agccgtcggg
     2281 cccggcggtc tcaccagtcc agaagtcggt gtagccggcc gcctcggcgg cctgcagcag
     2341 ctcccggtgg tcgcccagcg acacccccgg caacggcaaa gtcaatcccc aacgcccctc
     2401 cacgcgacaa ccgtatagcc tgcgggcgcc ctatggccga ggcctcccaa gcaaagcccg
     2461 accgcaatct tgcgctggag ttggtccgcg tgaccgaggc ggcggcgctc gcctccgccc
     2521 gctgggtcgg ccgcggcgac aagctcgccg ccgacggcgc cgccgtcgac gcgatgcgct
     2581 tcgtgctcga ctcggtctcg atggacggcg tcgtcgtgat cggcgagggc gagaaggacg
     2641 aagccccgat gctctacaac ggcgagcaga tcggcgacgg ctcgccgccg agcgtcgaca
     2701 tcgccgtcga cccgctggag gggacgcggc tctgcgcgct cgggatgccg agcgcgatcg
     2761 cggtgatcgc gctggccgag cgcggctcga tgttcgatcc cggcccctgc gtctacatgg
     2821 aaaagatggc gggcggggaa gagatcgccg acctgctcga tctcgaccgg ccgctgccgg
     2881 agaccttgaa gctgatcgcc gaccgcaagg gcggcacgat cggcgacgtg atcgtggtga
     2941 tgctcgaccg cccgcgccac gaggacgcga tccgcgaagt gcgcgaggcc ggtgcccgca
     3001 tccgcctgat caccgacggc gacgtctccg ccgccctgct ggcggtctcc gaacgctcgc
     3061 cggttgacct gctctggggg atcggcggca cccccgaggg cgtgatctcc gccgccgcgc
     3121 tgaagtcgat gggcgggcag ctgctcggcc gcctctggcc gcgcaacgac gaggagcggg
     3181 ccgcagccct cgacgccggc tacgacctcg accgcgtgct cacagccaac gacctctgtt
     3241 ccggcgagga cgtcttcttc tccgccaccg gcgtcaccga cggcgatgtg ctccagggtg
     3301 tccgctaccg cggcgccggc gtcaccacgg aatcgctggt gatgcgctcg cgctcgggca
     3361 ccgtgcggcg cgtccacgcg cgccacgatc gcgagaagct gcgcgcgatt ctcggcgagt
     3421 ccgcgaagcg ctacggctag ctgcggttga agttccccca cttgggttag agaagggggt
     3481 gtgatcgcgg tcgccgggtc gaccggctac atcggcggtt tgctctgccg gcagctgcgc
     3541 gaggctgggg aggaggtgcg agcgctcgcc cgcaatcccg agcgggccgg cgagttgacg
     3601 gagatcggct gcgaggtgtg ccaggccgac gtgctcgatc cctcgagcct gcgtaaggcg
     3661 ctggatggcg ctgacgtcgc ctactacctc gtgcactcga tggggagggg agccgacgac
     3721 ggtgactttg cagcgcgtga ccgccagggg gccgagaact tcgccgccgc cgcggccgac
     3781 gccggggtgc gcaggatcgt ctacctcggt ggccttggtg gcctcggcaa gggttccgag
     3841 catctcgcca gccgtcacgc gaccgccgaa accctgcgcg ccggcgccgt cccggtcgcc
     3901 tacttccgcg cggccgcggt gatcggggcc ggcagcgagt cctttcgcac cgtcttctac
     3961 ctggtccgtc gcctgccggt gatggtgacc ccacgctggg tcgtgacgcg gacgcagccg
     4021 atcgcggtcg acgacgcggt cggtttcctc gccgacgccc gcgacctcgg cctgccactc
     4081 gaccgcgagc tgcagatcgg cggtcccgac gtgaccacct acggcgggat gatcgacgag
     4141 ctcgcgcgcg ccctcgatcg ccgcccgccg ctgcggatta ccgttcccgt ccttacgccc
     4201 gcactatcgt cgctctggat cgggttggtg acgccggtcg acgcgggcgt cgcccgtccg
     4261 ctgatcgagg gcctcgccac cgagacggtg gtcaccgacc ccacgggaat ggagttgctg
     4321 ccggagcggg agcgcgttcc cctttcacaa gccctgcaca ttgcggcaga aaacgtaact
     4381 gttcgtttat agaacttttc tgttccaata aaaaactatt ggtactctga ctctgcaccg
     4441 aaagggggag taagggtgtc aagaggagct gaagtcccga ctacgaatgg ggccggaaat
     4501 caggcggtgg ggctggagcg gcccgaggcc gaccgcttcc gcgccggcga gcagattttc
     4561 acgatcctcg ccacgccgct caacgtgatg gttttgcggg ccctggcccg tcgcccgatg
     4621 cggctggcgg agctgaggca agcgaccggg ttgccggcgc agacgacgtt gcgcggccac
     4681 ctgaccagcc tcgccgagat cggcgcggtg agcaaacggc cgaccgcgca gatgccgtac
     4741 gcggtggaga accagttgac ccagatgggc gaggatctgc tcgcggtcgc cgatcggctc
     4801 gaggcatggc tggcgctcgc gccggaagcc ccgatctcgc tggaagccgg tggcgcccgc
     4861 ggcgtcgtca aggcgttcat cgacggctgg ggatcgacga tgatgcgcgg tctggcggcg
     4921 cggccgatgt cgttgaccga gctcgaccgg cagatccccg acctcagcta cccggcgctc
     4981 gagcggcgcc tctccagcat gaggatggcc gggctgatcg aagcccagcc gggcaccggt
     5041 ggcggcacgc cttacgcggt caccgattgg gcgcgccgtg gtgtgctgcc gctcgccgcg
     5101 gctgccgcct gcgaaggcac tcacctcgga aatgcggcgc ctctggtcag cgagatcgac
     5161 atcgaggcgg cgttcatgtt ggcgaccccg ctggtcgggc tgacgccgga tctggcgggc
     5221 tcgtgccagc tggaagtcga agcggagccc gaccactcgc gcgacgcctc cggcgtccag
     5281 gttgcggtcg aggggggcag ggtggtcgcc tgtgactgcg agctggggcc gagcccggcc
     5341 ggctacgcca ccggctcgac tgcccactgg ttcaacgcga tcctcgaggg cacagccagc
     5401 ctgctgagct ttggcggctc ccgccagctc agcgaagcga tggtcagcgg tcttcactcc
     5461 gcgcttgtga cccgctagcg accgcgcgcc cgcgctcgac ccgagcgacc tcttcgcctt
     5521 cgaacggcac caacagctcg aaggtgaggt cgggcagcag ctcgggcagg ctggcggcgc
     5581 gaccgagcac gatctcctcg tagacgcggg cgagcaccgc gccgaccccg aggctggtcg
     5641 cctggctcac gtccggggcg gcgggcgcgt cggggagacc ctcatcgagc agggcgacga
     5701 agcggctgaa ttcagcctgg aagcgaaccc gcgactcggt gcccattccc gccagctcga
     5761 ccagcatgaa tttggcggca ggcgggtcgg cggcgaacca ctcgagcgcc ctggccaggg
     5821 cgcgacgcat ccgttcgggc cactcgccgg gctccatgta ggcagcgcgg actttctctt
     5881 cgaggtcgtc ggtgagcagc gcgtgggcgg tgagggcgca ggtgcgcttg tcgtcgaaga
     5941 gttcgtagaa ggtctcgcgc ccgacgccgg cctcgtcgag gatgtcggcg acggtggtcg
     6001 ccccgtagcc cttggccgcg gtcgcgcgca cgaccgcggc cagcagccgt tcgcgctggg
     6061 agcgtgtgac cagctcgcgc ggcagtccgt ggcggccgcg cggcagccgc cacttgcgca
     6121 ggtcttcggg ctcgttggtc accggctcaa ggagcgctcg gggttggcgt cggcttcggc
     6181 ccggcgggcc tgcttcaagc cctcctcgtg accgacgaac ggggcgacgg cgaggtagac
     6241 ggtctccggc aggagatcgg gcagcgaggc cgccttgccg gcggcgatct ggttgaagat
     6301 cagcagctgg gcgcggccga aggcgaggtt ggggtccatg cggggctctc cctgctcgcc
     6361 ggcggcctcc cagagcccgg agacggcggg gatcagcagc cgccggtagc gctcgaccgc
     6421 ggcgggttcg accgcgggcg cgtcgccggt caggagctgg gccagccccg gcttctcggc
     6481 catcaaccgg acgatcgccg cggtcgccgc gagcaccgcg tcggccggtg ggtcggcagg
     6541 ggtgtgggcg ttgcgggcga ccgtccgcac ctcctcgatc ccggagtcga ccgcggcttc
     6601 gaagcaggcc cgcttgtcgg tgaagtgttt gtagaaagtc gtgcgcgaga tgctcgcctg
     6661 gccgactatg tcggagatcg tcgttccggg gaaagtcttc tccgcgcagc tcgcgaccat
     6721 cgcgtcgatg atccgcgccc gctgagcccg tgccgcgatg tcggtgggtg tcttggggtc
     6781 aagaacgcct gacaccgttc caaatatatg acagttttta ttgcctaccg cggcacgtcg
     6841 acgttatctg ccgcagcctg gggggattcg tcgcatccct ggcagggccg cgcggaggcc
     6901 cggtaaggga gttcgccggt gtcgtggagc tcgtggcgca acgaattgat tacggctccg
     6961 accatcagcg cgaggctgag gacgtagaac cagagcagcg cgatcaggat gaagccgagg
     7021 gtcgagccga agcgcgagag gctcgagacg gtcgagaggt agatcgggaa gagccagttg
     7081 gcgaggccgg cggcgaaggt gacgaaggcg gcgccgggcc agaccgcgcg ccagggcatg
     7141 tggcctttcg gcaccgccca gaagatcacc gagcagatcc cgaaggtgac cagcagggcc
     7201 gccgcaagca gcagcaccgt gtcgatcgcc ttgatgctgg agagcccgaa ggggagccgg
     7261 tcggtgctgg agagcagggc gctctcgacc gccggcacga agatgctggc ggcgatgaag
     7321 aggacgacga cgaagagcat cgcgaaggag aagcgcttct gctcgatcca gccgcggcac
     7381 tcgacgtggt agatccggca gaaggcggtg tccatcgcgc cccagaacga ggcgccgatc
     7441 cagagcgagc cgacgaaggc ggcgatcccg atcgtggtcg agttgttctc gatccggttg
     7501 aggacgtcgg tcagggtgtc ctgctcgacc gagggaaaga gccgctgcag gtcgctcagc
     7561 acgctggtct cgaccccctc gatcttgagt acctggccga aaacgaagag cacgagcagg
     7621 gcgaacggga agaccgccag catcaggttg taggcgacca tcgccgagag cccggtgacg
     7681 ttctcctcgt acgcgcggcg ccaaaaggcc tgcagccagg ggcgtggatt gcgcagtgag
     7741 ctgggcattg actttacaaa ctgaagtggc tactatgcga acaggcgttt gagcaagttt
     7801 gacgcccgcc cattagggca aactgaactg atggcaaccc ttactttcgc ggacccttcc
     7861 tctggtccgc tttttcatcg cccggcgcga ctttcggctc gcgacgggat ttattgaccc
     7921 aggactaaac agcaagcgct agggaaacga gtagacacgg atgctggtag cagagcttca
     7981 ggagttggag gaggtcaaga acctcatcaa tcgcggtcag cagcttggcg tgctgacctt
     8041 cggcgacgtc gcctcggcgg tctccgaggt cgatctcgac gagtctgacg tcgaggacct
     8101 ctacggccac ctcgagaagt cggggatcga gctcgtcgag gacatggacc cggcccagaa
     8161 agcggcggct gacgcaatgc ccgccgacac tgggaaaaaa gggcgtcggc gccagaaaca
     8221 ggcgctcgac ctgaaaccgg acatgaccac ggacagcttg cagctgttcc tcaaggacat
     8281 cggcaaagtc cgcctgctga ccgcggcgga ggaggtcgaa ctcgccaagc ggatcgagcg
     8341 cggcgacctc gacgcgaagc agaaaatggt cgagtcgaac ctgcgactgg tcgtctcgat
     8401 cgcgaaaaat tatcggaacc agggcctgcc cttcctcgac ttgatccagg agggaacgct
     8461 gggcctggtg cgcgcggcgg agaagttcga ctaccgcaag gggttcaagt tctccaccta
     8521 cgcgacctgg tggatccggc aggcgatcgc acgcgccctg gccgacaagg cgcggacgat
     8581 ccgcatcccg gtccacgtgg tcgagaagct gaacaagatc ggtcgcgccg agcgcaagct
     8641 ggtcaccgag ctgggccgtg agccgacccc ggaggagatc gccgaggtca ccggcatcga
     8701 ccccgaggag gtcgactcga tcaagcgctc cgcgcaggcc ccggtctcgc tggagaagcc
     8761 ggtcggtgac gaggaggagt cggagttcgg ccagttcatc gccgacgaga aggccgagtc
     8821 gccgttcgat cgcgccgccg acctgctcac caaagaggcg ctgaaggagg ccctcgagaa
     8881 cctctcctac cgcgagcgcc gcgtgctcga gctccgttac gggctcggcg gcgagcaccc
     8941 gcgcaccctg gacgaggtcg gccgcacctt caacgtcacc cgcgagcgga tccgccagat
     9001 cgagaaccag tcgctgaaaa agctccagag tctcggcgag gcccaaaaac tccgcgaggt
     9061 cgcctgaggg cgtcgctgcc ggcgtcctcg gtcggctggc tcgggtcacg cacctaagtg
     9121 cgctcccctc gccagcctcc ctgcgtcctt ggcagcgacg ccctcagcaa ccttgcaagc
     9181 cgcatagcag cagggtttaa gccgtagaac atcccggccg atataggtcg ggatgttcga
     9241 catcgactca gccctccgta cccttgtcga gcgggagggg tctgacctgc atctgaaggt
     9301 cggatcgccc ccggttgcgc gcatgcacgg ggaactgatc ccgctggagg gattcggcga
     9361 cctcgccgac gccgacaccg agaaggcgct gcatgacatc gccgaggtcc gctcgctgac
     9421 cgaattcgag gaggaaggcg aggccgactt ctcctacgcg ttggagggcg tctcgcgttt
     9481 ccgcgtcaac tccttcaaac agcgcggctc gatctcgatc gtctgccgcg cgatcccgtt
     9541 cacgatccgc tcgatcgacg acctcgggct gcccgacgtg gtccgctcgc tggccgagga
     9601 ggagcgcggc atcgtcctcg tcaccggcac caccggctcg ggcaagagca cgaccctggc
     9661 ggcgatgatc gaccacatca acctgaaccg ggcgcggcac atcatcacgc tcgaggaccc
     9721 gatcgagtac ctgcaccccg acaaacgctc gatcgtcaac cagcgcgaag tcggcttcga
     9781 caccaagggc ttcgggcggg cgatgcggcg ggtcctgcgc caggaccccg acgtgatcct
     9841 gatcggcgag atgcgagacg aggagaccgt gcgcacggcg ctctccgcgg ccgagaccgg
     9901 ccacctcgtg ctctcgaccg tgcacacgct ggacgcgacc gagacgatca accggatcat
     9961 cgacttcttc ccaccccacc tgcagcagca ggcgcgggta atgctggcgg cgaccctgaa
    10021 gggggcgatc gcccagcgcc tcgtgcccga cgtgaccggc gagggccgcg tgccggcctc
    10081 cgaggtgatg gtcgtcaccg gccgggtcca ggacctgatc atgaacccgg aggagaccgg
    10141 caagatcacc gaggtcatcg ccgaaggcag ctattacggg atgcggacct tcgaccagtc
    10201 gttgctcggc tacgcgatgg agggccggat cagtgagcgg acggcgatgg agttcgcctc
    10261 cagccctcac gacttcaagc tgatgctcgc cgccggcggc cagcgcgcca gcggcatcga
    10321 gcagatggaa acggcggcgt cgtgagggag aagctgaaca acgacccgaa gatgcaggtc
    10381 gcgctggttg cggttctggt ggtcgccgcc ctcctcttct tcctcaaatc gagcggcggc
    10441 gaagaagaag gtgaatcggg cggcgcggtg gcgaccgtga acggcgaaac tgcgaccggc
    10501 tcgaccccgg gcgaagctgt cgacgcggcg atcgaagggc tcgaaacggg cgccgtcgaa
    10561 gccggcgcca gcgccgtgcc ggcctcggtc ccagccccgc cgcccccgcc ggcattcacc
    10621 tccgcttacg acgccaacaa gaccgttgtc ctcctggtcg tccacgacgc cgggatcgac
    10681 gaccggctga ccgcgaaggc cgccgacgcg gtcgaaggga tggccgaggc cgagctgatc
    10741 cgggtgccgc tgaagcagct gccgcgctac gccgcggtca ccgtcgggct ggagctgaac
    10801 cgggtgccgg cactggtcgt cgtgcggccg cgcgggctc
//
LOCUS       DASRIG010000034        21204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_17306, whole genome shotgun sequence.
ACCESSION   DASRIG010000034 DASRIG010000000
VERSION     DASRIG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17306"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFB52_00790"
     CDS             <1..783
                     /locus_tag="VFB52_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZO04898.1"
                     /translation="VSAAQLAGLGISRATVSGWVRRGRLHRLHRGVYAVGHHSPNEWQ
                     RFMAAVLACGKRAALSHQSAAYLWGFLKPEGGPVHVTSPSRSGKVSQRGIVLHRSPSL
                     KREGEVTRRERIPVTTPRRTIVDLEQTLEPYLVRRARRQAEFLKFELNLPTDRSRSDL
                     ESDFLAFLAKHGFPRPEVNVKVGKHRVDFLWRAHKLVVETDFFDYHRGHQAFEDDHQR
                     ELDLRLAGYTVRRYTGAQLDDYPAEIVVELGEVLGGGAEVRR"
     gene            complement(794..1957)
                     /locus_tag="VFB52_00795"
     CDS             complement(794..1957)
                     /locus_tag="VFB52_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04899.1"
                     /translation="MLASSLTSSSSAVTAQEKYDAAQNRLEGVRADQSSLAETIAAQN
                     KAIDSMIGEVSALRLELAAVEAELAAKQEELEQATKALEAEEEHLEEVRDKLQRTLGI
                     LRERLVAIYEAGTPNVLNAILESEDWSEMSAQAEYLNQIQSYDDSVVERVKSLRDEVT
                     DAVARLTDNRDKVEASRDSVAAIEREVAARSAEAEARFADLQAAQAERRATMNQLGSE
                     EERINDSLAQLGQEVAEEGGPVPAGAQVPAPLNPSEQAGFISESEASAPANAPQAVKD
                     AIAAANAIASTPYVWGGGHGSFESSGYDCSGAVSYALHGGGFLESPLDSTGLESWGEP
                     GAGKWITVYANAGHTWMIIAGLAFDTVGGPGPRWHSSPTSSTEGFVVRHPAGY"
     gene            complement(2186..2533)
                     /locus_tag="VFB52_00800"
     CDS             complement(2186..2533)
                     /locus_tag="VFB52_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO04900.1"
                     /translation="MLEIFFFVIWIWILITILSDLFRDHELSGWWKAVWVLFLVFIPF
                     LTALIYLIARGSGMRDRTIKAQADAKKHFDEYVREQAHTSPADELHKLNDLKEKGALS
                     DAEFEQAKAKLLA"
     gene            complement(2642..3388)
                     /locus_tag="VFB52_00805"
     CDS             complement(2642..3388)
                     /locus_tag="VFB52_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZO04901.1"
                     /translation="MGDDLTQRFRGARRDSGLAVLEGFHALKHALRFGAELEVAAAED
                     PAELERLAAELAPDLAGEFETRVQMVAPGTLAALVPQAPRTGVVAIARRPAVDVAAVL
                     ADPRPAPVVLLEQPRNMGNMGACVRVAAGADAAAVLTTGENDPWNPDAIRGGAGLQYA
                     LPVAAVGEELPASDRPLLAIDPEGEDLRPAELPARAVLAFGTERHGLSEPLLARADAR
                     IAIPMRPGVSSLNLATSVAAVLFAWRLSDG"
     gene            complement(3432..4814)
                     /locus_tag="VFB52_00810"
     CDS             complement(3432..4814)
                     /locus_tag="VFB52_00810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZO04902.1"
                     /translation="MAARFAIKPTLRAKGQSEARAAGVLLMAGSVFVLLSVVLPHPEG
                     SDTGDLIATGVAMAALGVLAFGASPRVPLLLTHGLIALTAALTGLLIYESAIAAGQYG
                     SIWVWATLIVAYYFPRRVAAAHVAWILVVYAVALAVVPNTAGYSPVTRWLFTGISLCV
                     VMTFTSYIVARRARADRRARSFFDLSQDMLCTLDSSGRIVEVNDAWEAHLGYAPEELQ
                     GRHLIDLTHEDDRERAVAEAALVFEGKPSAGLENRVWAKDGSLHWLRSSAALAVEEDM
                     LYARATDVTELKRVEDEREELLGQVAEMARSDALTGLPNRRALADGMPREMARARRAG
                     SDLCLAVVDIDRFKVFNDTHGHLAGDRLLRECAIAWDGVVRGEDLLVRFGGEEFLVVL
                     PDCALDEAAEIVERLRAATPGGQTCSAGVTVWDGVEDFDAMVARADSALYMAKAGGRD
                     CLVQAPLVES"
     gene            complement(4848..6281)
                     /locus_tag="VFB52_00815"
     CDS             complement(4848..6281)
                     /locus_tag="VFB52_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HZO04903.1"
                     /translation="MLDEDHVELAIRETEVQILPGRKTRMWTYGGSFPGPTIRRRAGR
                     RTKVTFRHELPAAGELSVHLHGGHNRTQFDGQPGGLTKRQPVSYYCRIPRGLSERASG
                     NDLLLSPGRSKTYVYDLREGGRPERASFQWYHDHRLDRTAPNVWKGLAGMWIVDDAFE
                     EGLPLPRGDRDLPLLITDRQFDRNNQLTDPFADGRRPPADGISAPTILVNGAFMPHHR
                     VRACRYRLRVLNASQFRSYNLRLSNGAPMVQIATESGLMPRPIRREQILLGPAERVEL
                     IVDFAAARGETVELRSSAHHPGRNPEGAHTYNGALMQFRVGADRVADRTRVPRRLRPL
                     PEWAEHVARQPDRKPDKVWKIEIAGFFKPTWTINGRTFNPARSEAFPALDTTEIWEIH
                     NRTAVAHVMHIHHTDWYLLARDGKPPPPWEDCLKESFFVYPGERIRVAGRFSDYTGKF
                     VLHCHMLDHEDHGLMGQFQVVKSSTRA"
     gene            complement(6475..7005)
                     /locus_tag="VFB52_00820"
     CDS             complement(6475..7005)
                     /locus_tag="VFB52_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04904.1"
                     /translation="MAAVLSVFAAPVGAAEVPSTGVTAEIDMVFEKGKVKFVAPQTVT
                     QGDTLKIVNSTDPKKIGPHTFSLVKKGVLPKTAPARKACFSPKHICLSIAEWHKFNPK
                     TEKIGLDPVEVGPKGWSTAGDNSKKGDSWFTEKKGETFEQQVTAAPGDLYFICAVHSW
                     MQGKVKVVAPTPVPAL"
     gene            7201..7581
                     /locus_tag="VFB52_00825"
     CDS             7201..7581
                     /locus_tag="VFB52_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO04905.1"
                     /translation="MANKWLHTCYRIGDIDRSVAFYEKLGFEENRRMPIGDEAINVFM
                     GMPDDPGRLELTYNHGISEYDHGTAYNHIALLVDDLDATLAQLSSEGIDPEKPPYRPG
                     GRTEGHRIAFVRDPDGYRIELIGE"
     gene            7652..7912
                     /locus_tag="VFB52_00830"
     CDS             7652..7912
                     /locus_tag="VFB52_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04906.1"
                     /translation="METLAVSTGVAIAYTVVLFVLLIIGPLVVTALKGQWLLFAAGWL
                     APGLVWTITALRLGRPGSWWARRFYGPRKMARAEDRYGPVSP"
     gene            complement(7909..10050)
                     /locus_tag="VFB52_00835"
     CDS             complement(7909..10050)
                     /locus_tag="VFB52_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZO04907.1"
                     /translation="MATRTRRRHRKPHHPIWHKIAIPIAVVFALLLVAGGIAAAWAIK
                     VYNDAPALDELKKVGKGTSSSIYAADGSLIGFIRSNNVRQPVTYEELPATLREATIAI
                     EDKNFYEHGAVDYEGVFRAVWEDIKAGGKPVQGASTITQQLVRNLYIQNPDDTVERKI
                     REAKLAYELEDKHSKRWILKNYLNYAPYGTVEGQTVVGAEAAAQTYFSKPAKELGLVE
                     AALIAGLPQAPSEYNPFLDPKAATGRRNEVLNQMYEQGYISASEKREAQESGLNLRPG
                     DKYRVIHDQFLFDLVQQQLIDRYGLATVRNGGLKAYTTIDPDLQQKAEEAVASCYVCY
                     PEGGPAAALASVDPETGAIVALASTEGSASEEQFNYAWQAHRQPGSSFKTYVLATAIK
                     QGIDPETTYYDGTSPAVLDLPYGGTWEVSNSEPTEGGTMSLDEATVHSVNVIFAKLDL
                     DVGSEEVTKTAEEMGIEAPLESVPAEGIGGLSYGVTPLEMADGYATLANGGVHHDPTA
                     VSKVVFPDGRVDEIATDKGKRVLTEGQAYEVTRVLEGVITSGTGSGYTSIGCGAEAGK
                     TGTSEELSDAWFVGYTPMFSTAVWVGHPQSREATGYGGPTAGPIWQSYMLAAQEGECP
                     QFEIPEDMPELESYSSDHTGTAADAYDPSEEEFEVEDEGKGKKDQGDEEAEGEPEDEL
                     PETNPPPESSPPSGGGGGGSPPSVGGGISPG"
     gene            10219..10575
                     /locus_tag="VFB52_00840"
     CDS             10219..10575
                     /locus_tag="VFB52_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZO04908.1"
                     /translation="MKLVIGIVRPEQANQVLEALYRAEVKGFSISRVQGHGGELDRVE
                     TYRGTTVKMGLEEKVRFEVAVSDSFVQPTIDALCEGARTGEVGDGKIFVLPLERAIRI
                     RTGDEDVEAVTPVGKS"
     gene            10572..11876
                     /locus_tag="VFB52_00845"
     CDS             10572..11876
                     /locus_tag="VFB52_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZO04909.1"
                     /translation="MSAVTAAVAGVDTGDTAWMLVATALVLMMTPALGLFYAGLVRSK
                     NTLNTFMMCVAAIAVATVTWAAVGYSLAFDGTGDIIGGLNHAFLGGVDFAPREGTTIP
                     EMVFFAFQATFCIITVALVSGAVVERMRFGPFLVFAALWSILVYAVLAHWAFGGGWLQ
                     EGGTLDFAGGIPVEMGSGFSALAAALVVGARKDYGRQALLPHNAVYVLLGAGLLWFGW
                     FGFNGGSGFSTGDPSVLAFTNTLLTPACTLVVWFALDLIRGRKVTAIGAATAIIVGCV
                     AITPAGGFISPGWAMLLGALAALPSYAVIVWRTRTHVDETLDVLAAHGTAGLFGILFI
                     GFFAEVAWNGVSDGLLYGNAAQLWDQVLAAIAAPVYAFVATFVLLKVINAVMPLRASA
                     KEEAQGMDVVQHGEEAYVTGEGAILVSPEDGGQIPVAVADSR"
     gene            11892..12215
                     /gene="cutA"
                     /locus_tag="VFB52_00850"
     CDS             11892..12215
                     /gene="cutA"
                     /locus_tag="VFB52_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZO04910.1"
                     /translation="MACEYLQLLTTTGSEDEAAAIAAVLVERRLAACVQIAGPIKSIY
                     RWQGEIENAREWQCVAKTKASHWDAVEAAIREKHSYDEPEIIATPIAAGSAGYLRWID
                     ESLSD"
     gene            complement(12216..12983)
                     /locus_tag="VFB52_00855"
     CDS             complement(12216..12983)
                     /locus_tag="VFB52_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04911.1"
                     /translation="MSAAQIRFATLWRREVNRFMKIKKQTLGAPLLETFLYISVFGAA
                     LGTRIDKLHGIDYIVFVVPGLIMMAWSTNAFTNNSSSILQQKFQGAIQDQLSSPASPL
                     ELLLAFSLGGFMRGLIVAVITFVASVILIDLPVDHVLVLIPALFLVGFFFSQLGVLIG
                     VRAEQFDDVAFAQTFVLTPLIFLGGVFYSATLLPQPWETITHFNPIYYMINLVRYGFV
                     GYTEVSIPLSLAALTAATVALFLVNLRLFSRGYKLRS"
     gene            complement(12980..13780)
                     /locus_tag="VFB52_00860"
     CDS             complement(12980..13780)
                     /locus_tag="VFB52_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO04912.1"
                     /translation="MSDPAPGNDPALHIVDLEKRYPTGVEALRGVSLDIEPGQLFGLL
                     GPNGAGKSTLIHCTTGLAQPTGGSIQVFGHDATTGSYAEARQAVGLAPQEPNLDWFLT
                     VAETLDYHAGYFGMPKRERRERTEELLETFSLTEKRDERTRTLSGGMKRRLILARAVM
                     HKPRLLILDEPTAGVDVELRLELWHYVQRINEEGTTILLTTHYLEEAEQLCDRIAFIT
                     EGQIVAEGTNEEVAERFGVANLEDAYLALVGRKELSRSHIELGDEVAS"
     gene            complement(13809..14471)
                     /locus_tag="VFB52_00865"
     CDS             complement(13809..14471)
                     /locus_tag="VFB52_00865"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZO04913.1"
                     /translation="MSFPSAIAPPPLALGPKWLEPEFWIETFGMLGVLAVVFIESGLL
                     VGFLLPGDSLLFTAGLLSANDVLPDLWVLLVTIPLAAIAGDQVGYAIGRKSGPAVFSR
                     PDSRFFRQEYVVKAEEFFAAHGPRTIILARFVPIVRTLAPVMAGVARMPYRTFVVYNV
                     IGGVAWGVGLTTLGYFLGQIEFVHDNIEPIILGIVALSLIPVVHELLRARRSAKGSGQ
                     AD"
     gene            complement(14485..15780)
                     /locus_tag="VFB52_00870"
     CDS             complement(14485..15780)
                     /locus_tag="VFB52_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04914.1"
                     /translation="MARLTTPRALLLALAVSLIALIAAAPAGAAPAVSGEFDVPGLGS
                     NNKLVQGPDGNMWVTTGGGGKDVAKITPAGVVTEYAIEAVTPSGITVGPEGLIWVARS
                     GGVVSFNPANPEGTKKVTDIGELGATPSITLGPDGNLWAVGEGKEKVVRIPPANPGSP
                     TAFPVAGLNGPKDIDAAGSLVVIAAFEHIYAVDMTGAVVGDQKIAGQAQGVGGNPNGQ
                     YAFTQPVNTPKEIGLLAPGAAPIIRSAEGTDPFGIALGSDGAYWSPEFISDGLLRMTA
                     DGTVTALKGFAKNSAPRQIAAGPDNTLWVTLEMTKKVGRVSGLEPPIGPPPPATKPQT
                     QIKKGPKGKVTTKGKKAKVSFKFASTDAAATFQCRIVKVKKKGAAKSAKAPAFKGCKS
                     PKKYTLKPGKYRFEVRAVNVAGADPTPAKRSFRVVRVAG"
     gene            15843..16721
                     /locus_tag="VFB52_00875"
     CDS             15843..16721
                     /locus_tag="VFB52_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HZO04915.1"
                     /translation="MAAPLLAVDAPSMLFRAFYALPSKIVGTDGKPVNALLGTANLIL
                     REVEAHEPRAVVLCFGPDAAAYRTALYPAYHENREAEMPDELAPQFEKARDFFAAFNW
                     TVATSGDLEADDLLGTYARREAEAGGHALLLTGDRDMYQCAGEGVTVLYVRTGGKGAE
                     LVGPEQVRERYGIGPELVPDFIALRGDPADGLPGAKGVGEKTAAELLKKHGSLEAILD
                     NAIREQRPKLRASLIEGREDILKFKEIATLRDAGVEPPPDAPTDWAGAAAAARERGMN
                     RLAERLETADPGRGSA"
     gene            complement(16760..16999)
                     /locus_tag="VFB52_00880"
     CDS             complement(16760..16999)
                     /locus_tag="VFB52_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04916.1"
                     /translation="MDQVVQIAGALLILTAFAAVQFERMRPDSRVYLTLNLVGSVILA
                     VLAVVEQMWGFVLLEGVWVIVSAWGLFSLLGGRSR"
     gene            17033..17878
                     /locus_tag="VFB52_00885"
     CDS             17033..17878
                     /locus_tag="VFB52_00885"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HZO04917.1"
                     /translation="MGAQTIPWRGPGPDRPDLPLPPGKMPLRSSGQTRKRWRYVGFYG
                     EQLMLCAARAQVGPLSQSFWVLWEREGGEQLSHTAMRPGSREVWMDGPRVEIDARGLR
                     ASLTLGEAAPIETICPSSGGAGWGWTRKRAGVPFTGEVEASGRKWKVNALGVDDESAG
                     YHQRHTSWHWSAGVGSAADGRAVAWNLVEGINDPPSASERAVWVEGEPHEPDPVRFDG
                     LAAVVFGDGGRLEFSSESERARNENFLLVQSRYRHRFGGFSGSLDGIELASGLGVMEQ
                     HDAVW"
     gene            complement(18045..18782)
                     /locus_tag="VFB52_00890"
     CDS             complement(18045..18782)
                     /locus_tag="VFB52_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04918.1"
                     /translation="MPSPAAVIAIVALVFAVSGVAVALPGKNSVATKDIKKNAVTAAK
                     IKNGSVTEAKLGDGAVTTSKIGNDAVTGAKVNEATLGKVPSATSAETATKATSAEKAT
                     TAENAVELEGRTLSQVRPIAVGTKDESAQGLDDLSFENVMGVNITLPTGGADVVLNAA
                     LELDNESGGQAGGACRLKFNGSVVSSLVNITMTDEHNYALALTAIDANAGAGTRQALV
                     ECQGSIGDDDVKFVNGDLTVTAYPIGS"
     gene            complement(18917..>21204)
                     /locus_tag="VFB52_00895"
     CDS             complement(18917..>21204)
                     /locus_tag="VFB52_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO04919.1"
                     /translation="PGCDRALLEALAAASGPQLIRPRVLSVEATARLVAGRLGRAADP
                     EFSAACHGATGGNPFLLRELAGALRADGSRGGAEEVSQIRDLGPNTVSRSLLLRLTAM
                     PPAAGAIAAAVAVLGNDVELHHAAEFAGVELGEAALAVDSLADAEIFAPELPLNFVHP
                     IVRSAVYLDLGPGERAAAHARAARLLVTNGAGAELVAPHLIHSAPAGEQRFVELLREA
                     AAAALGRGAPETAAVYLRRAREEDEAARADAELLCELGTAEYLTGEQPEAAVAHLEEG
                     IALSPDPELRSEAWLALSRTALSLDVPAAVAVLEEAVEDLAGHESERVRRLETELNCV
                     GVTHADSFRRSAARIDALPELPGETPAERLVLANKAYRSGQAGEDLAGTIDLCRRALA
                     GGSLIAEEGLESSAVTQVLYVMYMADLTDEALAHAGAGLERAIARGSGWGFVSASGVR
                     AALHYLAGDMRATEADSRQALELPGCPPFAVPFVSAFLALALVERGELEEADAVVDAA
                     GCGPDLPPIIHMELLLWARARLRAAQGRDAEALADLADYGARCDRVGAHLPGIPWRGD
                     AALAAARRGEAERARELSADQLRRALAWGTGSAVGRAQMVEGLVGGGERGLALLADAV
                     ESLAASPARLDQARALVEYGAALRRAGQRREAREHLRDGMEAARRCGATALVDRAHEE
                     LVTAGARPRRLMFSGVEALTASEGRVAAMAAQGMGNREIAQSLFVTVKTVENHLGRAY
                     AKLGIHSREELPAALAGESAA"
BASE COUNT         3081 a         7216 c         7561 g         3346 t
ORIGIN      
        1 gtgagcgcag cacagctcgc cgggcttggg atctcgcggg caaccgtctc ggggtgggtg
       61 aggcgcgggc gcctgcaccg cctccaccgt ggcgtttacg cggtgggtca ccactctccg
      121 aacgaatggc agcgctttat ggcggcggtg ctcgcctgcg ggaaacgcgc ggcgctgagc
      181 catcagagcg cggcttatct ctgggggttc ctcaagccgg aaggtggacc cgtccacgtc
      241 acctcaccga gcaggagcgg caaggtctct cagcgtggga tcgtgcttca tagatctccc
      301 tctctgaaga gagaaggaga agtgactcgg cgtgagcgga tcccggtgac gacgccccgg
      361 aggacgatcg tggacttgga gcaaacgctc gagccttatt tggtgaggcg cgccagaagg
      421 caggcggagt tcttgaagtt cgagctgaac cttccgaccg atcgctcacg ctctgacctg
      481 gagagcgact tcctcgcgtt ccttgcaaag cacggattcc cccgccccga ggtgaacgtc
      541 aaggtcggca agcaccgggt cgacttcctc tggcgcgccc acaagctcgt cgtcgaaacc
      601 gacttcttcg actaccaccg gggccaccag gccttcgagg acgaccatca gcgcgagctt
      661 gacctacgtc tcgcgggcta caccgtccgc cgctacaccg gagcccagct cgacgactac
      721 ccagccgaga tagttgttga gctgggcgaa gtcctgggcg gcggtgccga agttcgccgc
      781 tagccgaacc ggctcagtac ccagccggat gccgcacgac gaagccttcc gtcgagctag
      841 tcggcgacga gtgccacctt ggcccggggc cgccgaccgt gtcgaacgcc agcccggcga
      901 tgatcatcca ggtgtggccg gcgttggcgt agacggtgat ccacttgccg gcgcccggtt
      961 cgccccagct ctcgaggccg gtcgagtcga gcgggctttc gaggaagccg ccgccgtgca
     1021 gggcgtagct gaccgcgccc gagcagtcgt agccggagga ttcgaaggag ccgtggccgc
     1081 cgccccagac gtagggagtc gaggcgatcg cgttggcggc ggcgatcgcg tccttgaccg
     1141 cctgcggcgc gttggcggga gcgctggctt cgctttcgct gatgaagccg gcctgttcgc
     1201 tcgggttcag cggcgccggg acttgggcgc cggccgggac cggtccgcct tcctcggcga
     1261 cttcctgacc gagctgcgcg aggctgtcgt tgatccgttc ttcttcggac ccgagctggt
     1321 tcatcgtcgc gcggcgttcg gcctgcgcgg cctgcaggtc ggcgaagcgc gcctcggctt
     1381 cggcgctgcg ggcggcgact tcgcgctcga tcgcggcgac cgagtcgcgc gaggcttcga
     1441 ccttgtcgcg gttgtcggtc agccgggcga ccgcgtcggt gacttcgtcg cgcagcgact
     1501 tcacgcgctc gacgaccgag tcgtcgtagc tctggatctg gttcaggtat tcggcctggg
     1561 ccgacatctc cgaccagtct tcggattcga ggatcgcgtt gagcacgttc ggcgtgccgg
     1621 cctcgtagat cgcgaccagc cgttcgcgca ggatcccgag cgtccgctgc agcttgtcgc
     1681 ggacctcttc gaggtgctct tcttcggctt cgagagcctt ggtcgcctgc tccagctctt
     1741 cctgcttcgc cgccagttcg gcctcgaccg cggccagttc caggcgcagc gccgagactt
     1801 cgccgatcat cgagtcgatc gctttgttct gcgcggcgat cgtttccgcc agcgagctct
     1861 ggtcggccct caccccttcg aggcgattct gggcggcgtc gtatttctcc tgcgcggtga
     1921 cggcggagga gctggaggtc agagagctgg ccagcacgca cagcgcgacc agcaggagcg
     1981 ccgcaaagac cagcgcccgg cgcttcgtcg cgcccggatg tgaactcgaa gccagcaggg
     2041 tcgcgcaggc tagcgaagtc gcctgaagat tgttaggagt tgctaataac ctgcaacttc
     2101 ccttgcaaat tgcggccttc tggaggaatc cgaaggccgt tgtcgaggtt cggggggcgg
     2161 cccgccgccg cccccgctcc cccgatcagg cgagcagttt cgccttcgcc tgctcgaact
     2221 ccgcgtcgct cagcgcaccc ttttccttca ggtcgttgag tttgtggagt tcatcggcag
     2281 gcgaggtgtg agcctgctcc cgcacgtatt cgtcgaaatg ctttttggcg tcggcttggg
     2341 ccttgatcgt tcggtcccgc atcccgctgc cgcgcgcgat caggtagatc agcgcggtta
     2401 agaacgggat gaagaccagg aacaggaccc agaccgcctt ccaccatccg gacagctcgt
     2461 gatcgcggaa gagatcgctc aggatcgtga tcaggatcca aatccagatc acgaagaaga
     2521 agatctcgag cacgatcaag aggaaatgcc cgaagctgat gtcagcgaac ggcaaggtgt
     2581 ttcctttcgg tcagaacaga aactgcggtg gcggcactag gccgggattc gtcaaagaac
     2641 gctatccgtc cgagaggcgc caggcaaaca ggaccgcggc gacgctggtc gcgaggttga
     2701 ggctggagac gccggggcgc atcgggatcg cgatccgggc gtcggcgcgc gcgagcagcg
     2761 gctcgctgag gccgtggcgc tcggtgccga aggcgagcac cgcgcgggcc ggcagctcgg
     2821 ccgggcgcag gtcctccccc tcggggtcga ttgccaacag cggccggtcg cttgccggca
     2881 gctcctcccc caccgcggcc accggcagcg cgtactggag gccggcgccg ccgcggatcg
     2941 cgtcggggtt ccaggggtcg ttctcgccgg tggtcagcac ggcggccgcg tcggcgccgg
     3001 cggcgacccg cacgcaggcg cccatgttcc ccatgttgcg cggttgctcg agcagcacga
     3061 ccggagccgg acgcgggtcg gcgagcaccg cggcgacgtc gaccgccggc cgccgcgcga
     3121 tcgcgaccac gccggtgcgc ggcgcctgcg ggaccagcgc ggccagcgtc cccggcgcca
     3181 ccatctgcac ccgcgtctcg aactcgccgg ccaggtcggg ggccagctcc gccgccagcc
     3241 gctccagctc ggccgggtcc tcggcggccg cgacctccag ctcggcgccg aagcgcagcg
     3301 cgtgcttgag cgcgtggaag ccctcgagca cggcgaggcc ggagtcgcgg cgggcaccgc
     3361 ggaagcgttg cgtgaggtcg tcgcccacgc gcgcatcatg accggatcgg cggcgcgccc
     3421 acgccgggcg ttcaggactc gaccagcgga gcctgcacga ggcagtcgcg accgccggcc
     3481 ttggccatgt agagcgccga gtcggcgcgg gcgaccatcg cgtcgaagtc ctcgaccccg
     3541 tcccagacgg tgaccccggc cgagcaggtc tgccctccgg gcgtcgccgc gcgcagccgt
     3601 tcgacgatct ccgcggcctc gtccagcgcg cagtcgggca ggacgacgag gaactcctcg
     3661 ccgccgaagc ggaccagcag gtcttcgccg cgcaccaccc cgtcccaggc gatcgcgcac
     3721 tcgcgcagca gccggtcgcc ggccaggtgg ccgtgggtgt cgttgaagac cttgaagcgg
     3781 tcgatgtcga cgaccgccag gcagaggtcg ctgccggcgc ggcgggcgcg ggccatctcg
     3841 cgcggcatcc cgtcggcgag cgcgcggcgg ttgggcagcc cggtgagcgc gtcgctgcgc
     3901 gccatctccg cgacctgccc gagcagctcc tcgcgttcgt cctcgacccg cttcaactcg
     3961 gtgacgtcgg tggcgcgcgc gtagagcatg tcctcctcga ccgcgagcgc ggcgctcgag
     4021 cgcagccagt gcaggctgcc gtccttcgcc cagacccggt tttcgagccc ggccgagggc
     4081 ttgccttcga acaccagcgc cgcctcggcg accgcgcgct cgcgatcgtc ctcgtgggtg
     4141 agatcgatca ggtggcggcc ctgcagttcc tccggtgcat agccgaggtg ggcctcccag
     4201 gcgtcgttga cctcgacgat ccggccgctc gagtcgagcg tgcagagcat gtcctgggag
     4261 aggtcgaaga agctgcgggc gcggcggtcg gcgcgggcgc ggcgggcgac gatgtagctg
     4321 gtgaaggtca tcaccacgca gagcgagatc ccggtgaaga gccagcgggt gaccggcgag
     4381 tacccggcgg tgttggggac gaccgcgagg gcgaccgcgt agacgaccag gatccaggcg
     4441 acgtgggcgg cggcgacccg gcgcgggaag tagtaggcga cgatcagcgt cgcccaaacc
     4501 cagatcgatc cgtactggcc ggcggcgatc gccgactcgt agatcagcag gccggtgagg
     4561 gcggcggtaa gggcgatcag tccgtgagtg aggagcaggg ggacgcgggg ggacgcgccg
     4621 aaggcgagca ccccgagcgc cgccatcgcc accccggtcg cgatcaggtc accggtgtcg
     4681 ctgccctccg ggtgcggcag cacaacggag aggagcacga agaccgagcc ggccatcagc
     4741 agcacacccg cggcccgggc ctcggactgg cccttggccc tcaaggtggg cttgattgcg
     4801 aacctcgccg ccatgcctaa acagcatcgg cgccacgccc ggcgacttta ggctcgggtg
     4861 gatgatttga ccacctggaa ctggcccatc aggccatggt cctcgtggtc gagcatgtgg
     4921 cagtgaagga cgaattttcc ggtgtaatcg gagaaccggc cggcgacccg gatccgctcg
     4981 ccgggataga cgaagaagct ctccttgagg cagtcttccc agggcggcgg tggcttgccg
     5041 tcgcgggcga gcaggtacca gtcggtgtgg tggatgtgca tcacgtgggc gaccgcggtg
     5101 cggttgtgga tctcccagat ctcggtcgtg tcgagcgccg ggaaggcctc cgagcgggcc
     5161 gggttgaagg tgcggccgtt gatcgtccag gtcggtttga agaagccggc gatctcgatc
     5221 ttccagacct tgtccggttt gcgatcgggc tggcgggcga cgtgctcggc ccactcgggc
     5281 agcggtcgca gccggcgcgg gacgcgggtg cggtcggcaa cgcggtcggc gccgacgcgg
     5341 aactgcatca gggcaccgtt gtaggtgtgc gcaccctccg ggttgcggcc ggggtgatgg
     5401 gcactggagc gcagctcgac cgtctcgccg cgggcagcgg cgaagtcgac gatcagctcg
     5461 acccgctcgg ccggcccgag caggatctgc tcgcggcgga tcggccgcgg catcagccca
     5521 ctctcggtcg cgatctggac catcggcgcg ccgttggaga ggcgcaggtt gtagctgcgg
     5581 aactgcgaag cgttgagcac ccgcaaccgg tagcggcagg cgcgcacccg gtggtggggc
     5641 atgaaggcgc cgttgaccag gatcgtcggc gccgagatcc cgtcggcggg cggccgccgc
     5701 ccgtcggcga acgggtcggt gagctggttg ttgcggtcga actggcggtc ggtgatcagc
     5761 aacgggaggt cgcggtcgcc gcgcggcaac ggcagccctt cctcgaaggc gtcgtcgacg
     5821 atccacattc ccgccaaccc tttccagacg ttgggagcgg tgcggtcgag gcggtggtcg
     5881 tggtaccact ggaacgaggc gcgttcggga cggccgccct cgcgcaggtc gtagacgtag
     5941 gttttgctcc gccccggcga gagcagcagg tcgttgccgg aggcgcgctc gctgagcccg
     6001 cgcgggatgc ggcagtagta ggagaccggc tggcgcttgg tcaggccgcc gggctggccg
     6061 tcgaactggg tgcggttgtg gccgccgtgc aggtgcacgg agagctcgcc cgccgccggc
     6121 agctcatggc ggaaggtgac cttggtgcga cgaccggcgc ggcggcggat cgtcggcccg
     6181 ggaaagctgc cgccgtaggt ccacatccgg gtcttgcgcc cgggcaggat ctgcacctcc
     6241 gtctcgcgga tcgccagctc gacgtggtct tcgtcgagca cccgcgggat cggcagccgc
     6301 cggcggaacg gaacccccgc ctgggccagc tccggcccgg cgccgagctc gggcagcagc
     6361 cctcccccgc ggccgccgag cggcagcagc aggccgaggg cgccgccgcc gagggcgccg
     6421 aggaaagctc ggcgccccag gcgtggcgat gcgtgtgact gcgtgtccgt ggcgctagag
     6481 ggccgggacg ggcgtcggcg cgacgacctt gaccttgccc tgcatccagg agtggacggc
     6541 gcagatgaag tagaggtcac ccggcgcagc ggtcacctgc tgctcgaagg tctcgccctt
     6601 cttttcggtg aaccaggagt cgcccttctt cgagttgtcg ccggcggtgc tccaaccctt
     6661 cggaccaacc tcaaccgggt cgagcccgat cttttccgtt ttcgggttga acttgtgcca
     6721 ttcggcgatc gagaggcaga tgtgtttcgg cgagaagcag gccttccgcg ccggcgcggt
     6781 tttcggcagc acgcctttct tgaccagcga gaaggtgtgc gggccgatct ttttcgggtc
     6841 ggtgctgttg acgatcttca gcgtgtcgcc ctgggtgacc gtctgcggcg cgacgaattt
     6901 gactttgccc ttctcgaaga ccatgtcgat ctcggcggtg acaccggtcg agggcacttc
     6961 ggcggcgccg acgggagcgg cgaagacaga caaaacagcg gccacggagg ccgtgagcaa
     7021 cagggcacgg ccggaggccc tgcggggcag caacttgaac aagagacacc tttctgtgtg
     7081 atttgacgcg tgaaacgaac gacgcgagct tgggcaaact attcgctccg tcggcgccga
     7141 gcaactgact ttcgtctcaa acccgtacga cttttggcaa cccctaggta gccttcggga
     7201 atggctaaca agtggctcca cacctgctac cgaatcggcg acatcgaccg ctcggtcgcc
     7261 ttctacgaga agctcggctt cgaggagaac cggcggatgc cgatcggcga cgaggcgatc
     7321 aacgtattca tgggcatgcc cgacgacccc ggccggctcg agctgaccta caaccacggg
     7381 atcagcgagt acgaccacgg caccgcctac aaccacatcg cgctcctggt cgacgacctc
     7441 gacgcgacgc tcgcccagct ctccagcgaa gggatcgacc ccgagaagcc gccctaccgc
     7501 cccggcggcc gcaccgaggg acaccggatc gcattcgtcc gcgaccccga cggctaccgg
     7561 atcgagctga tcggcgagta ggacggctgc ttcggtgcgt ccagcgggcg tccgcgccgc
     7621 ccagcgaaac tcctgacagg attgcgccgg catggagacg ctcgcggtat caaccggggt
     7681 cgcgatcgcc tacacggtcg tgctgttcgt gctgctgatc atcggcccgt tggtggtcac
     7741 ggccctgaag gggcagtggc tgctcttcgc cgccggctgg ctggcgcccg gactggtctg
     7801 gacgatcacg gcgctgcgcc tgggacgacc cggctcttgg tgggcgcgcc gcttctacgg
     7861 gccgaggaag atggcccgcg ccgaagaccg ctacggcccg gtcagccctt agcccgggga
     7921 aatgccgccg cctaccgagg gcggcgagcc accgccgccg ccacctgacg ggggcgagga
     7981 ttcaggcggc gggttcgttt cgggcagttc gtcttccggt tcgccttcgg cttcctcgtc
     8041 gccctggtct tttttgcctt tgccctcgtc ttcgacttcg aattcttctt cggaggggtc
     8101 gtaggcgtcg gcggccgtgc cggtgtggtc gctgctgtag ctctccagtt ccggcatgtc
     8161 ttccgggatc tcgaactgcg ggcattcgcc ttcctgggcg gccaacatgt agctctgcca
     8221 gatcgggccg gcggtcgggc cgccgtagcc ggtcgcttcg cgcgactgcg ggtggccgac
     8281 ccagacggcg gtcgagaaca tcggcgtgta gccgacgaac caggcgtcgg agagctcctc
     8341 ggaggtgccg gtcttgccgg cttccgcgcc gcagccgatc gaggtgtagc cggagccggt
     8401 ccccgaggtg atcacgccct cgagcacccg ggtgacttcg taggcctggc cttcggtcag
     8461 cacccgcttg cccttgtcgg tggcgatctc gtcgacgcgg ccgtcgggga agacgacttt
     8521 gctgaccgcg gtcgggtcgt ggtggacgcc gccgttggcg agggtcgcgt agccgtcggc
     8581 catttcgagc ggcgtgaccc cgtaggagag accgccgatc ccctcggccg ggaccgattc
     8641 gagcggcgct tcgatcccca tttcttctgc cgtcttcgtc acttcctcgg agccgacgtc
     8701 gaggtcgagc ttggcgaaga tcacgttgac cgagtgcacg gtcgcttcgt cgagcgacat
     8761 cgtgccgccc tcggtcggct cggagttgct gacctcccag gtgccgccgt agggcagatc
     8821 gaggaccgcc ggcgaggtgc cgtcgtagta ggtcgtctcg gggtcgatcc cctgtttgat
     8881 cgccgtcgcc agcacgtagg tcttgaacga cgagcccggc tggcggtgcg cctgccaggc
     8941 gtagttgaac tgttcttcgg aggcggatcc ttcggtcgaa gcgagggcga cgatcgcgcc
     9001 ggtctccggg tcgaccgagg cgagcgcggc ggccggcccg ccttcgggat aacagacgta
     9061 acaggacgcg accgcttctt cagccttctg ctgcagatcc gggtcgatcg tcgtgtaggc
     9121 cttgagcccg ccgttgcgga ccgtggcgag gccgtagcgg tcgatcagct gctgctggac
     9181 cagatcgaag aggaactggt cgtggatcac ccggtacttg tcgccggggc gcaggttcag
     9241 cccgctttcc tgggcctcac gtttttccga ggcgctgatg tagccctgct cgtacatctg
     9301 attcagcacc tcgttgcggc ggccggtcgc ggctttcggg tcgaggaagg ggttgtattc
     9361 ggaaggcgcc tgcggcagtc cggcgatcag cgccgcttcg accaggccga gctccttcgc
     9421 cggtttcgag aagtaggtct gcgccgccgc ctcggccccg accacggtct gaccttcaac
     9481 cgtcccgtag ggcgcgtagt tgaggtagtt cttgaggatc cagcgcttcg agtgcttgtc
     9541 ctcgagttcg taggcgagct tcgcttcgcg gatcttgcgc tcgaccgtgt cgtcggggtt
     9601 ctggatgtag aggttgcgga ccagctgctg ggtgatcgtc gaggcgccct ggaccggctt
     9661 gccgccggcc ttgatgtcct cccagacggc acggaagacg ccttcgtagt cgaccgcgcc
     9721 gtgctcgtag aagttcttgt cctcgatcgc gatcgtcgcc tccctcagcg tggccggcag
     9781 ctcctcgtag gtgaccggct ggcgcacgtt gttggagcgg atgaagccga tcaggctgcc
     9841 gtcggcggcg tagatcgagg aggaggttcc cttgccgact ttcttcagtt cgtcgagcgc
     9901 gggcgcgtcg ttgtagacct tgatcgccca ggcggcggcg atcccgccgg cgacgaggag
     9961 gagggcgaag accacggcga tcgggatcgc gatcttgtgc cagatcgggt ggtgcggctt
    10021 gcggtgccgc ctgcgcgtac gcgtggccat cggcccgcga ttgtagagat tcgggcaccc
    10081 cggcccttct gccccgacag cgcgcaagcc cgcttgctga gcggatttag cagggattta
    10141 ggtccggagt ccgacggcgt ctttagggtg cccgcagcgt catgtcgagg cgcctcattg
    10201 ggagagaggg gaggaacgat gaaactcgtg atcgggatcg tccggcccga gcaggcaaac
    10261 caggtgctgg aagcgctcta tcgcgccgag gtcaagggct tttcgatcag ccgtgtgcag
    10321 ggccacggcg gtgagttgga ccgggtcgag acctaccgcg gcacgaccgt gaagatgggc
    10381 ctcgaggaga aggtgcggtt cgaggtcgcg gtctccgact cgttcgtgca gccgaccatc
    10441 gacgcgctct gcgagggcgc ccgcaccggc gaagtcggcg acggcaagat cttcgtgctg
    10501 ccgctcgagc gcgcgatccg gatccgcacc ggcgacgagg acgtcgaagc ggtcaccccg
    10561 gtgggcaagt catgagcgcc gtgaccgcag cggtcgccgg cgtcgacacg ggcgataccg
    10621 cctggatgct ggtggcgacg gcgctggtgc tgatgatgac cccggcgctg gggctcttct
    10681 acgccggcct ggtgcgctcg aagaacaccc tcaacacctt catgatgtgc gtcgccgcga
    10741 tcgcggtggc gacggtgacc tgggcggcgg tcggctactc gctcgccttc gacggcaccg
    10801 gcgacatcat cggcggcctc aatcacgcct tcctcggcgg cgtcgacttc gccccgcgcg
    10861 aagggacgac gatcccggag atggtcttct tcgccttcca ggcgaccttc tgcatcatca
    10921 ccgtcgcgct ggtctccggc gcggtggtcg agcggatgcg cttcgggccg ttcctcgtct
    10981 tcgccgcgct ctggtcgatc ctcgtctacg ccgtgctcgc ccactgggcg ttcggcggcg
    11041 gctggctgca ggagggcggc acgctcgact tcgccggcgg catcccggtc gagatgggct
    11101 ccgggttctc ggccctggcg gcggccctcg tggtcggcgc gcgcaaagac tacggtcgcc
    11161 aggcgctgct cccccacaac gccgtctacg tgctgctcgg cgccggcctg ctgtggttcg
    11221 gctggttcgg gttcaacggc ggcagcggct tctcgaccgg cgacccgagc gtgctcgcct
    11281 tcaccaacac gctgctgacc ccggcctgca cgctggtcgt ctggttcgcg ctcgacctga
    11341 tccgcggccg caaggtgacc gcgatcggcg ccgccaccgc gatcatcgtc ggctgcgtcg
    11401 cgatcacccc cgccggcggc ttcatcagcc ccggctgggc gatgctgctc ggcgcgctgg
    11461 cggcgctgcc gagctacgcg gtgatcgtct ggcggacccg cacccacgtc gacgagacgc
    11521 tcgacgtgct cgccgcccac ggcaccgccg gcctcttcgg gatcctcttc atcggcttct
    11581 tcgccgaggt cgcctggaac ggggtctccg acgggctcct ctacggcaac gccgcccagc
    11641 tctgggacca ggtactggcc gcgatcgccg ccccggtcta cgccttcgtc gccacctttg
    11701 tcttgctgaa ggtgatcaac gcggtgatgc cgctgcgcgc gagcgcgaag gaggaggcgc
    11761 agggcatgga cgtcgtccag cacggcgagg aggcgtacgt gaccggcgag ggtgcgatcc
    11821 tcgtctcgcc cgaggacggc ggccagatcc cggtcgccgt cgccgattcc cgctgagcga
    11881 gaggaggccc gatggcctgc gagtacctcc aactgctgac cacgaccggc tccgaggacg
    11941 aggcggcggc gatcgccgcc gtgctggtcg agcgccggct cgccgcgtgc gtgcagatcg
    12001 ccgggccgat caagagcatc taccgctggc agggcgagat cgagaacgcc cgggagtggc
    12061 agtgcgtcgc caagaccaag gcctcgcact gggacgcggt cgaggccgcg atccgcgaga
    12121 aacactccta cgacgagccc gagatcatcg ccaccccaat cgccgccggc tccgccggct
    12181 acctgcgctg gatcgacgag tcactcagcg actgactacg agcgcagctt gtagccgcgg
    12241 ctgaagaggc gcaggttgac caggaagagc gccacggtgg cggcggtcag cgccgccagc
    12301 gagagcggga tgctgacttc ggtgtagccg acgaacccgt agcggaccag gttgatcatg
    12361 tagtagatcg ggttgaagtg ggtgatcgtc tcccacggct gcggcagcag ggtggccgag
    12421 tagaagacgc cgccgaggaa gatcagcggg gtgaggacga aggtctgggc gaaggcgacg
    12481 tcgtcgaact gctcggcgcg gacgccgatc aggacgccga gctgggagaa gaagaaaccg
    12541 accaggaaga gcgccgggat cagcacgagg acgtggtcga cgggcaggtc gatcaggatc
    12601 accgaggcga cgaaggtgat caccgcgacg atcagcccac gcatgaagcc gccgagcgag
    12661 aaggcgagca gcagctccag tggcgaggcg ggcgaggaga gctggtcctg gatcgcgccc
    12721 tggaacttct gctggaggat cgaggacgag ttgttcgtga aggcgttggt cgaccaggcc
    12781 atcatgatca gcccggggac gacgaagacg atgtagtcga tcccgtgcag cttgtcgatc
    12841 cgggtaccga gcgcggcgcc gaagaccgag atgtagagga aggtttcgag cagcggcgcg
    12901 cccagcgtct gtttcttgat cttcatgaag cggttgacct cgcgccgcca gagcgtcgcg
    12961 aagcggatct gggcggcgct cacgaggcca cctcgtcgcc gagctcgatg tgcgagcgcg
    13021 agagctcttt gcgcccgacc agcgccaggt aggcgtcctc gaggttggcg acgccgaagc
    13081 gctcggcgac ctcctcgttg gtcccttcgg cgacgatctg accttcggtg atgaaggcga
    13141 tccggtcgca gagctgctcc gcctcctcca ggtaatgggt ggtgagcagg atcgtcgtcc
    13201 cttcctcgtt gatccgctgc acgtagtgcc agagctccag ccgcagctcg acgtcgacgc
    13261 cggcggtcgg ctcgtcgagg atcagcaggc gcggcttgtg catcactgcg cgggccagga
    13321 tcaaccgccg tttcatcccg ccggagagcg tgcgggtgcg ctcgtcgcgc ttctcggtca
    13381 gcgagaacgt ctccagcagc tcttcggtgc gctcgcgccg ctcgcgcttg ggcatcccga
    13441 agtagccggc gtggtagtcg agcgtctcgg cgacggtcag gaaccagtcg aggttcggct
    13501 cctgcggcgc cagcccgacc gcctggcgcg cctcggcgta gctgccggtg gtcgcatcgt
    13561 ggccgaagac ctggatcgag ccgccggtcg gctgcgccag cccggtcgtg cagtggatca
    13621 gcgtcgactt gccggcgccg ttgggaccga gcaggccgaa cagctgcccc ggctcgatgt
    13681 cgagcgagac accgcgcaga gcctccaccc cggtggggta gcgcttctcg agatcgacga
    13741 tgtggagtgc cgggtcgttg ccgggtgccg ggtcgctcat gggcagcgaa ccaacctaat
    13801 ggaccggatc agtcggcttg cccagacccc ttggcagagc ggcgcgcgcg gagcagctca
    13861 tgcacaactg ggatcagcga gagggcgacg atcccgagga tgatcggctc gatgttgtcg
    13921 tggacgaact cgatctggcc gaggaagtag ccgagcgtgg tcagccccac cccccaggcg
    13981 acgccgccga tcacgttgta gacgacgaag gtccggtagg gcatccgcgc caccccggcc
    14041 atcaccggcg ccagggtgcg gacgatcggg acgaagcggg cgaggatgat cgtgcgcggc
    14101 ccgtgcgcgg cgaagaactc ttccgccttg accacgtact cctggcggaa gaagcggctg
    14161 tcggggcggc tgaacacggc cggtccgctc ttgcggccga tcgcgtagcc gacctggtcg
    14221 ccggcgatcg cggccagcgg gatcgtcacc agcagcaccc agaggtccgg cagcacgtcg
    14281 ttggcggaga gcaggccggc ggtgaagagc agcgagtcgc cgggcagcag gaacccgacc
    14341 agcagcccgg actcgatgaa gacgacggcg agcacgccga gcatcccgaa ggtctcgatc
    14401 cagaactccg gctccagcca cttcgggccg agggcaagcg gcggcggcgc gatcgcggaa
    14461 gggaagctca tgcagcgcgg gacgtcagcc tgccacgcgg acgacgcgga aactgcgctt
    14521 ggcgggcgtc gggtcggcgc cggcgacgtt cacggcccgc acttcgaagc ggtacttgcc
    14581 cggcttcagc gtgtatttct tcggcgattt gcagcctttg aaggcgggag cttttgccga
    14641 tttcgccgcg cctttcttct tcaccttgac gatccggcac tggaaggtgg cggcggcgtc
    14701 ggtcgaggcg aacttgaagc tgaccttggc ttttttgcct ttggtcgtga ccttgccctt
    14761 cgggcctttt ttgatctggg tctgcggttt ggtcgccggg ggcggcgggc cgatcggcgg
    14821 ttcgagcccg gagacgcggc cgactttttt cgtcatctcc agcgtcaccc agagcgtgtt
    14881 gtcggggccg gcggcgatct ggcgcggcgc ggagtttttg gcgaagcctt tgagcgcggt
    14941 gaccgtgccg tcggcagtca tccgcaggag cccgtcggag atgaactcgg gcgaccagta
    15001 ggcgccatcg gagccgagcg cgatcccgaa cgggtcggtg ccttcggccg agcggatgat
    15061 cggcgcggcg cccggcgcca gcagcccgat ttccttcggc gtgttgaccg gctgggtgaa
    15121 cgcgtactgc ccgttcgggt tgccccccac accctgcgcc tggccggcga ttttctggtc
    15181 gccgaccacc gcgccggtca tatccacggc gtagatgtgc tcgaaggcag cgatcacgac
    15241 cagggagccg gccgcgtcga tgtccttcgg cccgttcagg ccggcgaccg ggaacgcagt
    15301 cggggagccg ggattggcgg gcgggatgcg aacgactttt tctttgcctt cgccgaccgc
    15361 ccagaggttc ccatcgggac cgagcgtgat cgaaggcgtg gcgccgagtt cgccgatgtc
    15421 ggtgactttt ttcgtccctt ccgggttggc ggggttgaag ctgaccacgc cgccgcttct
    15481 cgcgacccag atcagacctt cggggccgac cgtgatccca gacggggtca cggcttcgat
    15541 cgcgtattcg gtgacgacgc cggccggggt gatcttggcg acgtctttgc caccgccgcc
    15601 cgtggtcacc cacatgttcc cgtcggggcc ttggaccagc ttgttgttcg agcccagccc
    15661 cggcacgtcg aattcgccgc tgaccgcggg cgcggcgccg gcgggcgcgg cggcgatgag
    15721 ggcgatcagc gagacggcga gcgcgagcag cagtgcgcgg ggggtggtca agcgggccac
    15781 ggcgccagtc taaacaggcc cgcgggaccg ctgttgggat cttcgacaca tagtctggac
    15841 gggtggccgc cccgctcctc gccgtcgatg caccctcgat gctattccgc gccttctacg
    15901 cgctgccgag caagatcgtc ggcaccgacg gcaagccggt caacgccctg ctgggcaccg
    15961 caaacctgat cctgcgcgag gtcgaagccc acgagccgcg cgccgtcgtg ctctgcttcg
    16021 gccccgacgc cgccgcctac cggaccgcgc tctaccccgc ctaccacgag aaccgcgagg
    16081 cggaaatgcc cgacgagctg gccccgcagt tcgagaaggc gcgcgacttc ttcgcggcgt
    16141 tcaactggac ggtcgcgacc agcggcgacc tcgaggccga cgacctgctc ggcacctacg
    16201 cccgccgcga ggccgaggcc ggcggccacg ccctgctgct gaccggcgac cgcgacatgt
    16261 accagtgcgc cggcgagggc gtgaccgtgc tctacgtgcg caccgggggc aaaggcgccg
    16321 aactggtcgg gcccgaacag gtgcgcgagc gctacgggat cggccccgag ctggtccccg
    16381 acttcatcgc cctgcgcggc gaccccgccg acgggctgcc cggcgccaag ggcgtcggcg
    16441 agaaaaccgc ggccgagctg ctcaagaagc acggctcgtt ggaggcgatc ctcgacaacg
    16501 cgatccgcga gcagcggccg aaactgcgcg cctcgctgat cgaagggcgc gaggacatcc
    16561 tcaagttcaa ggagatcgcc accctgcgcg acgccggcgt cgagccccct cccgacgcgc
    16621 ccaccgactg ggccggcgcg gccgctgcag cccgcgagcg cggcatgaac cgcctcgccg
    16681 agcggctcga gacggccgat cccggcaggg gttccgctta actttttcgc gacgcgaaaa
    16741 agtacaagac gctccacccc taccgggatc ggcctccgag caagctgaaa aggccccagg
    16801 cggagacgat cacccagacg ccctcgagca ggacgaagcc ccacatctgc tcgaccaccg
    16861 cgagcacggc gaggatcacc gagccgacca ggttgagcgt caggtagaca cgcgagtccg
    16921 ggcgcatgcg ttcgaactgg accgcggcaa aggcggtgag gatcagcagc gcgccggcga
    16981 tctgtacgac ctggtccacc ctcccagcct aagcaccggg taccgtggcc gggtgggggc
    17041 gcagacgatt ccgtggcggg ggcccgggcc ggatcggccc gatctgccgc tgccgccggg
    17101 caagatgccg ctgcgctcca gcggccagac ccgcaagcgc tggcgctacg tcggcttcta
    17161 cggcgagcag ctgatgctgt gcgcggcgcg cgcccaggtc ggcccgctct cgcaatcctt
    17221 ctgggtgctc tgggagcgcg agggcggcga gcagctctcg cataccgcga tgcgcccggg
    17281 cagccgcgag gtctggatgg acgggccgcg ggtcgagatc gacgcgcgcg ggctgcgcgc
    17341 cagcctcacg ctcggcgagg cggccccgat cgaaacgatc tgccccagct cagggggggc
    17401 cggctggggc tggacgcgca agcgcgccgg cgttccgttc accggcgagg tcgaagcctc
    17461 gggccgcaag tggaaggtca acgcgctcgg ggtcgacgac gagtcggccg gctaccacca
    17521 gcgccacacc agctggcact ggtcggccgg cgtcggcagc gccgccgacg gccgcgccgt
    17581 cgcctggaac ctggtcgagg ggatcaacga cccgccgagc gcctcagagc gcgcggtctg
    17641 ggtcgagggc gagccgcacg agccggaccc ggttcgcttc gacggcctcg ccgcggtcgt
    17701 cttcggcgac ggcggccggc tcgagttcag ctccgagtcc gagcgcgccc gcaacgagaa
    17761 cttcctgctc gtccagtccc gctaccgcca ccggttcggc ggcttctccg gctcgctcga
    17821 cgggatcgag ctggcctccg ggctgggcgt gatggagcag cacgacgccg tctggtgagg
    17881 ggccgagcgc tcctccagga gcagctccag cgcccgggcg aagccgagcc agccgttgaa
    17941 cccgcggctg gccccggcct cgtcgccgag cgttccccgg atcgacccct cgctcttctc
    18001 gatgcgcagg gtcagggttc ggggtggagt ggacatcggg ctgcctagga accgatcggg
    18061 taggccgtca cggtcaggtc accgttgacg aatttgacgt cgtcgtcgcc gatcgagccc
    18121 tggcattcga ccagcgcctg gcgggtgccc gcaccggcgt tggcatcgat cgcggtcagc
    18181 gccagggcgt agttgtgttc gtcggtcatc gtgatgttga ccaggctgct gacgaccgag
    18241 ccgttgaatt tgagccggca ggcaccgccc gcctggccgc cggattcgtt gtcgagctcg
    18301 agcgccgcgt tgaggacgac gtcggcgccg ccggtcggca gcgtgatgtt gactcccatc
    18361 acgttctcga agctcaggtc gtcgagcccc tgcgcggatt cgtctttggt cccgacggcg
    18421 atcggcctca cctggctcag ggtccggccc tcgagctcga ccgcgttctc cgcggtcgtc
    18481 gctttctccg ccgaggtcgc tttggtcgcg gtctccgccg aggtcgccga gggcactttg
    18541 ccgagcgtcg cctcgttgac cttggccccg gtgacggcgt cgttgccgat cttcgaggtg
    18601 gtcaccgcgc cgtcgccgag ctttgcctcg gttaccgagc cgttcttgat tttcgccgcg
    18661 gtcacggcgt ttttcttgat gtccttggtg gcaaccgagt tcttgcccgg cagcgcgacg
    18721 gcgacaccgc tgaccgcgaa gaccagggca acgatcgcga tcaccgcagc cgggctcggc
    18781 aaccgccgga acctgctcaa cctgctcatc gagtgagacc tccagatagt gggtggaggc
    18841 cgatcgtcgc gagcgccgcc cagaccctca tgaggggcgc gcccctcact tttgggagtg
    18901 cacccccagg cgccgctcag gcggcggatt cgccggccag agccgccggc agctcctcgc
    18961 gcgagtggat cccgagcttc gcgtaggcgc ggccgaggtg gttctcgacc gtcttcacgg
    19021 tgacgaacag cgactgcgcg atctcccggt tgcccattcc ctgcgcggcc atcgcggcga
    19081 cccgtccctc gctcgcggtc agcgcctcga ccccggagaa catcagccgc cgcggccgcg
    19141 cccccgcggt gaccagctcc tcgtgggcgc ggtcgacgag cgcggtggct ccgcagcgcc
    19201 gtgccgcctc catcccgtcg cgcaggtgct cgcgcgcctc gcggcgctgc ccggcgcggc
    19261 gcagcgccgc cccgtactcg accagtgccc gcgcctggtc aaggcgcgcc ggcgaggccg
    19321 ccagcgactc gaccgcgtcg gcgagcagcg ccagcccccg ctcgccgcca ccgaccaggc
    19381 cctcgaccat ctgcgcccgg ccgaccgccg agccggtgcc ccaggccaag gcccgccgca
    19441 gctggtcggc ggagagctcg cgcgcccgct cggcctcacc ccggcgggcc gccgccaggg
    19501 cggcgtcacc gcgccagggg atccccggca ggtgggcgcc gacccggtcg cagcgggccc
    19561 cgtagtcggc gaggtcggcg agcgcctcgg cgtcgcggcc ctgcgcggcg cgcagccgcg
    19621 cccgcgccca cagcaacagc tccatgtgga tgatcggtgg caggtccggc ccgcagccgg
    19681 cggcgtcgac caccgcgtcg gcctcctcga gctcaccccg ctcgaccagc gcgagggcga
    19741 ggaaggcgga gacgaacggg accgcgaacg gtgggcagcc gggcagctcc agcgcctggc
    19801 ggctgtcggc ctcggtcgcg cgcatgtcgc cggccaggta gtgcagcgcg gcgcgcaccc
    19861 cggaggcgga gacgaagccc cagcccgagc cgcgggcgat cgcccgttcg agcccggcgc
    19921 cggcgtgcgc gagcgcctcg tcggtgaggt cggccatgta catcacgtag aggacctggg
    19981 tcacggcgct cgactccagc ccctcttcgg cgatcaaact gccgccggcg agggcgcgcc
    20041 ggcagagatc gatcgtcccc gccaggtcct ccccggcctg accgctgcgg taggccttgt
    20101 tggcgagcac gaggcgctcg gccggggtct cgccgggcag ctccggcagg gcgtcgatcc
    20161 gcgccgccga gcgccggaag ctgtcggcgt gggtgacgcc gacgcagttc agttcggtct
    20221 cgaggcggcg cacgcgctcg ctctcgtgcc cggcgaggtc ctcgaccgct tcctccagca
    20281 ccgccacggc cgcgggcacg tcgaggctga gcgcggtccg gctcagcgcc agccaggcct
    20341 ccgagcgcag ctccgggtct gggctcaggg cgatgccttc ttcgagatgg gcgaccgccg
    20401 cctccggctg ctcgccggtc aggtactcgg cggtgccgag ctcacagagc agctcggcgt
    20461 cggcgcgcgc cgcctcgtcc tcctcgcggg cccgccgcag gtagacggcg gcggtttccg
    20521 gggcaccgcg gccgagcgcc gccgcggccg cctcgcgcag cagctcgacg aagcgctgct
    20581 cacccgccgg ggcgctgtgg atcaggtgcg gcgcgaccag ctccgccccc gccccgttgg
    20641 tgaccaggag gcgggcggca cgcgcgtggg cggcggccct ctcccccggc ccgaggtcga
    20701 ggtagacggc ggagcgaacg atcgggtgga cgaagttcag cggcagctcc ggggcgaaga
    20761 tctcggcgtc ggcgagcgag tcgaccgcca gcgccgcctc ccccagctcg accccggcga
    20821 actcggctgc gtggtggagc tcgacgtcgt tgccgagcac cgcgaccgcg gccgcgatcg
    20881 ccccggcggc cggcggcatc gcggtcagcc gcaggagcag cgagcgcgag accgtgttgg
    20941 gaccgaggtc gcggatctgg ctcacttcct cggcgccacc gcgggaaccg tcggcgcgca
    21001 gcgccccggc cagctcgcgc agcaggaacg ggttgccgcc ggtcgccccg tggcaggcgg
    21061 cggagaactc cgggtcggcg gcgcggccga ggcgcccggc gacgagccgc gcggtcgcct
    21121 cgacgctgag cacgcgcggg cggatcagct gcgggccgga ggcggcggcg agcgcttcca
    21181 gcagggcacg gtcgcagccg ggct
//
LOCUS       DASRIG010000035         6972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2630403, whole genome shotgun sequence.
ACCESSION   DASRIG010000035 DASRIG010000000
VERSION     DASRIG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630403"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1413)
                     /gene="obgE"
                     /locus_tag="VFB52_00900"
     CDS             complement(124..1413)
                     /gene="obgE"
                     /locus_tag="VFB52_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZO04920.1"
                     /translation="MYDKAKIWVEGGGGGNGCISFRREAHVPRGGPDGGDGGHGGDVV
                     LVCDASKRDLGALKGAKHFRAQRGRHGEGSNRQGARGEDFEIPVPPGTQAVTVDGSHV
                     DLVEQGQRAIVAHGGRGGHGNKRFTTSTRQAPRFAENGTKGEADWIELQLKLLADVGL
                     VGLPNAGKSSLLSRLTRATPKVADYPFTTLSPALGTIEGEDRQAILADIPGLIEGAAD
                     GAGLGHEFLAHIERCSMLVHLVDLAPMEGDPVAAYETVRAELSAHGTGLELLPELVVL
                     SKRDLLTSEQVEEAIAAWRERLGEGVLGVLAVSSATGEGLEDLRARILSELPQLQPAL
                     APAGEAGGEFEAEHRVYRPVGEGGYWVEREDDGGFRVLGRGVELLFERHDVKNEEALA
                     YLEQRLNEIGVISALTKAGFENGDDVRIGEHEFELHV"
     gene            complement(1435..2961)
                     /locus_tag="VFB52_00905"
     CDS             complement(1435..2961)
                     /locus_tag="VFB52_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04921.1"
                     /translation="MPARLLALVVVLASLALAVAAPAQADLYFWDNYGGDPDSVGYTT
                     SDGSAGGALNSSGVTVTGPEGMAIDTVTGRLYVASYNAGPGEEGQIQYINLDGSGGGV
                     FTAPGAPVGEPMGVAVDPVGRMIYWTNTDGESISWARLDGSAGGSMNLTGAPVGQMYK
                     IAIDPVNGKLYWFVQKESDNFIAYANLNNSGGGGTLDLTGAPALLGTRALAVDTVGGR
                     VYWSDGTSKLFGFASVVGGGGGSVNLGGAYYTNSYGFAVDPTAGRLYWPNYDSTTSRA
                     NGIGFASLTGGPGGNLTPPETSPWAGAQDLLVLKAPTGTVAPAITRAKNNRSQLACSA
                     GSWAADFAGSYVYQAPTTTAYRWLRNGKAVAGATTTKLTAKSPGKYVCQVTGANQAGS
                     TATSSKVVKVEASKAKLVTKKKVTVKAGGTATFAIKVNNLGDLKSKNAKLCALLPKAA
                     KGFLKPSKCKSLGRLKGKGKKSAKLKITALPAASGVYSVSFKLKGVAGKPAKAKIVVR
                     "
     gene            complement(2972..3226)
                     /gene="rpmA"
                     /locus_tag="VFB52_00910"
     CDS             complement(2972..3226)
                     /gene="rpmA"
                     /locus_tag="VFB52_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZO04922.1"
                     /translation="MAHKKGLGSSRNGRESNPNMLGVKVFAGQQVYGGEIIVRQRGTK
                     FWPGEGTGLGRDHTIFATRPGKVDFFTARKGRTIRVLAED"
     gene            complement(3229..3603)
                     /gene="rplU"
                     /locus_tag="VFB52_00915"
     CDS             complement(3229..3603)
                     /gene="rplU"
                     /locus_tag="VFB52_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZO04923.1"
                     /translation="MYAVIESGGKQYKVEEGTSLFVDRLDAKEGDKVSLRPVMFRGDD
                     VIADGKGLEKVKVEATVAEHVRGPKIKVFKYKAKKGYRKRAGHRSELTKLEVTSLKAG
                     KAAAKKAPAEKKADKKAEKEES"
     gene            complement(3606..5456)
                     /locus_tag="VFB52_00920"
     CDS             complement(3606..5456)
                     /locus_tag="VFB52_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZO04924.1"
                     /translation="MKKTILVSAESGETRVAVLESKTKGGKADVAELYMERRGRRSIV
                     GNIYKGKVDNVLPGMEAAFVDIGLERNGFLHVDEIVLPGGEQAPKRGRGSGRRINELI
                     KPGQEILVQVVKDPLKSKGARLSMNVSIAGRYLVYAPQGSGVGVSRRLADKERDRLRK
                     MVDRTYKGPGGLIVRTAAHGAKKSDFVREIAYLQKLNDVLERRSDETKAPAMVFQEAD
                     LSVRVLRDVFLNEFEKAIIDSEKQFERVTSFFQRTAPELVGGVELYKDSKPLFEKWKI
                     DKEIESTLNRRVDLPSGGYLIIDYTEALTVIDVNSGSFTGRGKGGLEETITKVNTEAA
                     EEAVRQLRLRDIGGIIVIDFIDMARASNRDKVLKTMRKALDADKSKSYVVEVSPLGLV
                     EMTRQNVTDGVREILTELCPTCHGEGVVLSAETVALEGLRKMRDIAAENADKEAFLVR
                     VNPKVARELADPESGLVELEADTGKQFHFEGGDALAIDTFEVVESGDRATIEERALPF
                     KVGEEVLVKIDEPHMYNADDAVARIDSYIVSVTGAGRFVGERKMVRIDEVERSSAIAS
                     LLGGESPNGASSNGDGGGELESAAAPRKRSRGRRGGRGRSRAKQGSKEDE"
     gene            complement(5513..6709)
                     /gene="rodA"
                     /locus_tag="VFB52_00925"
     CDS             complement(5513..6709)
                     /gene="rodA"
                     /locus_tag="VFB52_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZO04925.1"
                     /translation="MYQARTRNTRAAPFEVHTGIAERLGLAHLDWPLALAGIGLILFS
                     VFTLGQATSHDVPGNPGYYVDRQMIYGVLGVLGMLVLARIDYSRFRELRVGIYTFLCV
                     SISLVFVFGFAAKGSRRAFDLPFFSFQPSELGKLLLVLALAGFVIDGARRGSPVRRTL
                     RYLALGLAPAALVFLQPDLGTSLVFVAATLAVMYFGGVPWTHFAVIGGGIVALIAVVL
                     VAAPAVGTPILKGYQEERLTSFMNSGEHADGAAYQQTQSKIAIGSGQFDGRGEKATQT
                     RLSFVPERHTDFIFAVIGERYGFVGAAFVLSLYALLFWRALKITTLSKNSYGTLVAGG
                     IALSLLFQVFVNVGMNLGIMPITGIPLPLMSYGGSAVIATFLAIGVLQSINIQARVSQ
                     KDGFAW"
     gene            complement(6710..>6972)
                     /locus_tag="VFB52_00930"
     CDS             complement(6710..>6972)
                     /locus_tag="VFB52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZO04926.1"
                     /translation="EGTSYPVFGGFPIPVAGKTGTAQRPPHGDQAWYVVLAPYPNPRI
                     VTVVTIEEGGFGAQTAAPAALRILEAYFDKKATEVGGETVAE"
BASE COUNT         1067 a         2304 c         2315 g         1286 t
ORIGIN      
        1 ggcaaaggca agcccgagga cgccgggatg cgacgcggcc tgcatttgtc gcccagaggg
       61 ctacaaatgc aggccggtag ccgggtgcgt catccaggtg ggcctggtcg tggaactgct
      121 catctagacg tgcagctcga actcgtgctc gccgatgcgg acgtcgtcgc cgttctcgaa
      181 gccggccttg gtcagggctg agatcacgcc gatctcgttc agccgctgct cgaggtaagc
      241 cagcgcttct tcgtttttga cgtcgtggcg ctcgaacagc agctcgacgc cgcggccgag
      301 gacgcggaag ccgccgtcat cctcgcgctc gacccagtag ccgccctcac ccaccggccg
      361 gtagacgcgg tgctcggcct cgaactcgcc gccggcctcg ccggccgggg cgagcgccgg
      421 ctgcagttgc ggcagctcgc tgagaatgcg cgcgcgcagg tcttcgagcc cctcgccggt
      481 cgccgaggag accgcgagta cgccgagcac gccctcgccg agccgctcgc gccacgcggc
      541 gatcgcttcc tcgacctgct ccgaggtgag caggtcgcgc ttggagagca cgaccagctc
      601 cggcaggagc tcgagcccgg tgccgtgggc ggagagctcg gcgcgcacgg tctcgtaggc
      661 ggcgaccggg tcgccttcca tcggcgccag gtcgaccagg tggacgagca tcgagcagcg
      721 ctcgatgtgg gcgaggaact cgtggccgag cccggcaccg tcggcggcgc cctcgatcag
      781 accggggatg tcggcgagga tcgcctggcg gtcctcgccc tcgatcgtcc cgagcgccgg
      841 cgagagcgtc gtgaacgggt agtcggcgac cttcggcgtc gcccgggtca ggcggctgag
      901 cagcgaggac ttgcccgcgt tcggcagccc gaccaggccg acgtcggcga gcagtttcaa
      961 ctgcagctcg atccagtccg cctccccctt ggttccgttc tcggcgaagc gcggcgcctg
     1021 ccgggtcgag gtggtgaagc gcttgttgcc gtggccacct cgtcccccat gagccacgat
     1081 ggcgcgttgt ccctgctcga ccaggtcgac gtggctgccg tcgacggtga ccgcctgcgt
     1141 gcccggcggc accgggatct cgaagtcctc cccgcgggcg ccctggcgat tcgagccttc
     1201 gccgtgacgg ccgcgctggg cgcggaagtg cttggccccc ttcagcgccc cgaggtcgcg
     1261 cttggaggcg tcgcagacca gcaccacgtc gccgccgtgc ccgccgtcgc cgccgtcggg
     1321 cccgccgcgc gggacgtgcg cctcgcgccg gaagctgatg cagccgttgc cgcctccgcc
     1381 gccctcgacc cagattttcg ccttgtcgta catcgcctcc gtggaggcta gtgactaccg
     1441 aacgacgatc ttcgccttgg ccggcttacc ggcgacgcct ttcagcttga agctgacgct
     1501 gtagacgccg ctggccgcgg gcagcgcggt gatcttcagc tttgcgctct tcttgccttt
     1561 tcctttgagc ctgccgagcg atttgcactt cgacggcttc aagaagcctt tggcggcttt
     1621 gggcagcagc gcgcagagct tggcgttctt tgatttcagg tcacccagat tgttgacctt
     1681 gatcgcgaag gtggccgttc cgccggcttt gaccgtcacc ttcttcttcg tcaccagctt
     1741 cgctttcgag gcttcgacct tgaccacctt gctggaggtc gcggtcgagc cggcctggtt
     1801 ggcgccggtc acctggcaga cgtatttgcc gggcgatttc gccgtgagct tcgtcgtcgt
     1861 cgcgccggcg accgctttgc cgttgcgcag ccacctgtag gccgtggtcg tcggtgcctg
     1921 gtagacgtag ctgccggcga agtcggccgc ccaggagccc gccgagcagg cgagttgcga
     1981 gcggttgttc ttggcgcggg taatcgccgg agcaaccgtg ccggtagggg ctttgaggac
     2041 aagtagatcc tgagcgccgg cccagggcga ggtctccgga ggcgtgagat tgcctcccgg
     2101 cccgccggtc aggctggcga agccgatccc gttggctctg ctcgtggtgc tgtcgtagtt
     2161 cggccagtag agccggcccg cggtcggatc gaccgcgaag ccgtaggagt tcgtgtagta
     2221 ggcgccgccg aggttgacgg atccaccacc gcccccgacc acgctcgcga acccgaacaa
     2281 tttcgacgtg ccatcggacc agtagacgcg tccgccaacg gtgtcgacag cgagtgcgcg
     2341 ggtgcccagg agggccgggg cccccgtgag atcgagggtc ccgccaccgc cactgttgtt
     2401 caggttggcg taggcgatga agttgtccga ttctttctgg acgaaccagt agagcttgcc
     2461 gttgaccgga tcgatcgcga tcttgtacat ctggcccacc ggcgctccgg ttaggttcat
     2521 cgacccgccg gcactgccgt cgagccttgc ccaagagatc gactcgccgt ccgtgttggt
     2581 ccagtagatc atccgcccaa ccgggtccac agcgacgccc atcggttcac cgactggagc
     2641 gcccggggcc gtgaacacac cgccgccgct gccgtcgagg ttgatgtact gaatctgacc
     2701 ctcttcgccc gggcccgcgt tgtagctggc tacgtagagc cggccggtga cggtgtcgat
     2761 cgccatgcct tcgggaccgg tgaccgtcac cccggaactg ttcagcgctc ccccggcgct
     2821 gccgtcggag gtcgtgtagc cgaccgagtc cgggtcgccg ccgtagttgt cccagaagta
     2881 gagatccgcc tgcgcgggcg ccgcgacggc cagcgcaagg ctggcgagga cgacgaccag
     2941 agcgagcagc cgcgcaggca tgtcgggagt tctagtcctc ggcgaggacg cggatcgtgc
     3001 ggcccttacg ggcggtgaag aagtcgacct tgccagggcg ggtggcaaag atcgtgtggt
     3061 cgcggccgag gccggtcccc tcaccaggcc agaacttggt gccacgctgg cgcacgatga
     3121 tctcgccgcc gtagacctgc tggccggcga agaccttgac gccgagcatg ttcgggttcg
     3181 attcgcgccc gttgcggctg gaaccgaggc ctttcttgtg agccatctct aggactcctc
     3241 tttctcagct tttttgtcgg cttttttctc agccggggct tttttggcgg ccgctttgcc
     3301 cgctttcagg ctggtcacct cgagcttggt cagctcggac cggtggccgg cgcgtttgcg
     3361 gtagcccttc ttcgccttgt atttgaagac tttgatcttc ggcccgcgca cgtgctcggc
     3421 gacggttgcc tcgactttga ctttctcgag acctttgccg tcggcgatca catcgtcccc
     3481 acggaacatc accgggcgca gggagacttt gtcgccctct ttggcgtcga gccggtcgac
     3541 gaacagggac gtgccctcct cgaccttgta ctgcttaccg ccgctttcga ttaccgcgta
     3601 catgcttact cgtcttcctt gctgccttgt ttggcgcgcg aacggccccg gccacctcgg
     3661 cggccacgac tgcgtttgcg cggagcggcg gcggattcta gctcgccgcc gccatcgccg
     3721 ttgctcgatg ccccgttcgg ggactcgccg cccagcagcg aggcgatcgc cgaggagcgc
     3781 tcgacctcgt cgatccgcac catcttgcgc tcaccgacga agcgtccggc gccggtcacg
     3841 ctgacgatgt aggagtcgat ccgggcaacc gcgtcgtcgg cgttgtacat gtgcggctcg
     3901 tcgatcttga ccagcacctc ctcgccgacc ttgaacggca gtgcccgctc ctcgatcgtc
     3961 gcgcggtcgc ctgattcgac cacctcgaag gtgtcgatcg cgagcgcgtc gccgccctcg
     4021 aagtggaact gcttgccggt gtcggcctcc aactcgacca ggccgctttc gggatcggcc
     4081 agctcgcggg ccactttcgg gttgacgcga accaggaacg cctccttgtc agcgttctcg
     4141 gcggcgatgt cgcgcatctt gcgcaggccc tcaagtgcga ccgtctcggc cgagagcacg
     4201 acgccctcgc cgtgacaggt cgggcagagc tcggtgagga tctcgcgcac cccgtcggtg
     4261 acgttctggc gggtcatctc gaccaggccc agcggcgaga cctcgaccac gtagctcttc
     4321 gacttgtcgg cgtcgagcgc tttgcgcatc gtcttcagga ctttgtcccg gttggaggca
     4381 cgcgccatgt cgatgaagtc gatgacgatg atgccgccga tgtcgcgcag gcgcagctgc
     4441 cgcaccgcct cctcggcggc ctcggtgttg accttggtga tcgtctcctc gaggccgcct
     4501 ttgccgcgac cggtgaagct gcccgagttg acgtcgatca cggtcagcgc ctcggtgtag
     4561 tcgatgatca ggtagccgcc cgagggcagg tcgacgcgcc ggttcagggt cgattcgatc
     4621 tctttgtcga tcttccactt ttcgaacagc ggcttcgagt ctttgtagag ctcgaccccg
     4681 ccgaccagct ccggcgcggt gcgctggaag aagctggtca cccgttcgaa ctgcttctcg
     4741 gagtcgatga tcgccttctc gaactcgttg aggaagacgt cgcggaggac gcggaccgag
     4801 aggtcggcct cctggaagac catcgccggc gccttggttt cgtcgctgcg gcgctcgagc
     4861 acgtcgttga gcttctgcag gtaggcgatc tcgcgcacga agtccgattt tttggcgccg
     4921 tgcgcggcgg tgcggacgat cagcccgcct gggcctttgt aggtccggtc gaccatcttg
     4981 cgcagccggt cgcgctcttt gtcggcgagc cggcgggaga cgccgacgcc ggagccctgc
     5041 ggggcgtaga ccaggtagcg gccggcgatc gagacgttca tcgagagccg ggcgcccttc
     5101 gacttcagcg ggtccttgac cacctggaca aggatctcct ggccgggctt gatcagctcg
     5161 ttgatccggc gaccgctgcc gcgaccgcgc ttgggggcct gctcaccgcc cgggaggacg
     5221 atctcgtcga cgtgcaggaa tccgtttcgc tcgaggccga tgtcgacgaa ggcggcctcc
     5281 atgccgggca ggacgttgtc gaccttgccc ttgtagatgt tgccgacgat cgagcggcgc
     5341 ccacggcgct ccatgtaaag ctcggctacg tcggccttgc cgcctttcgt cttcgactca
     5401 aggacagcaa cgcgggtctc gccgctctcg gcggaaacca aaatcgtttt tttcattgaa
     5461 gaatcagctc agttcagtag gtacgcaacg ccggtgtccc tcatcggagg catcaccagg
     5521 cgaacccgtc cttctggctg actcgcgctt ggatgttgat gctctggaga actccgatcg
     5581 ctaagaacgt agcgatcacg gcagaccctc cgtagctcat caacggcaat gggatgcccg
     5641 tgatcggcat gatgccgagg ttcatcccaa cgttgacgaa gacctggaat agcagcgaga
     5701 gggcgattcc ccccgcgacc aaggtcccgt aggagttctt tgagagcgtc gttatcttca
     5761 gcgcccgcca gaagagcagg gcgtataagg acaggacgaa cgcggcgccc acgaacccgt
     5821 agcgctcgcc gatcaccgcg aagatgaagt cggtgtgccg ctcgggaacg aagctcagcc
     5881 gcgtctgggt ggctttttcg cctcggccgt cgaactgacc ggagccgatg gcgattttcg
     5941 attgagtttg ttggtaggcg gcaccgtccg catgctcgcc ggagttcatg aaggacgtca
     6001 gacgttcctc ctgatacccc ttgagaatgg gcgtgccgac cgcgggcgcc gcaactaaga
     6061 cgaccgcgat gagggcgact atgcccccgc cgattaccgc gaagtgggtc catggaactc
     6121 caccgaagta catcacggcg agggtggcgg cgacgaagac cagcgaggtc ccgaggtcgg
     6181 gctgcaggaa gacgagagcc gccggagcca gtcccagagc caaataacgc agcgtccgcc
     6241 gtaccggaga tccgcgccgg gcgccgtcga tgacgaagcc ggcgagggcc agtacgagca
     6301 ggagcttgcc gagctcggag ggctggaagg agaagaaagg caggtcgaaa gcgcgccgcg
     6361 agcctttcgc ggcgaagccg aagacgaaga ccaacgagat cgagacgcag aggaaggtgt
     6421 agatcccgac ccgcagctcg cgaaagcgcg agtagtcgat ccgggccagc accagcatcc
     6481 cgagcacccc gagcacgccg tagatcatct ggcggtcgac gtagtagccg gggttgccgg
     6541 gcacgtcgtg cgaagtcgcc tggccgaggg tgaacacgct gaagaggatc aggccgatcc
     6601 cggccagcgc cagcggccag tccaaatgcg cgaggccgag ccgctcggcg atcccggtgt
     6661 ggacctcgaa cggtgctgcg cgggtgtttc gggtgcgtgc ttggtacatc tattcggcga
     6721 ccgtttcgcc accgacttcg gtggctttct tgtcgaaata cgcttccagg atccgcagcg
     6781 ccgccggagc cgcggtttgg gcaccgaagc cgccctcctc gatcgtcacc accgtcacga
     6841 tacgcgggtt cggataaggc gcaagcacga cgtaccaggc ctgatctccg tgcgggggcc
     6901 gctgcgcggt ccccgtcttg cccgcaacgg ggatcgggaa accgccgaaa acggggtagg
     6961 aagtgccttc ct
//
LOCUS       DASRIG010000036         8015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3141056, whole genome shotgun sequence.
ACCESSION   DASRIG010000036 DASRIG010000000
VERSION     DASRIG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141056"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..457)
                     /gene="rpsI"
                     /locus_tag="VFB52_00935"
     CDS             complement(62..457)
                     /gene="rpsI"
                     /locus_tag="VFB52_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZO04927.1"
                     /translation="MESGARFLATGKRKRSIARVTLLPGDGKIEVNKRSLEEFFPRPL
                     HQTMARQPLTVSGYEGNVDVRIRVHGGGISGQAGAVRHGIARALTEIDPELRGDLKRR
                     GFLTRDARVKERRKAGFKKARKKPQFSKR"
     gene            complement(930..1367)
                     /gene="rplM"
                     /locus_tag="VFB52_00940"
     CDS             complement(930..1367)
                     /gene="rplM"
                     /locus_tag="VFB52_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZO04928.1"
                     /translation="MTVKTYVANAENRQRDWYVVDAEGKTLGRLATQIADALRGKRKP
                     EYTPHVDTGDFIVVVNAEKIRVTGDKLAQKTYWRHSGYPGGIKSRTLAEMLDRQPEEV
                     IRKAVKGMLPRNRLARQQLTKLKVYAGPDHPHQAQQPKPMETN"
     regulatory      1496..1657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1663..2601
                     /locus_tag="VFB52_00945"
     CDS             1663..2601
                     /locus_tag="VFB52_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO04929.1"
                     /translation="MRSTRTRTGKAGAITILALFALLATCAAAVAATGGAPTPTSTPP
                     PPGSGSGAPAAEPSAGAVTLLAAETAPRKSFYYGYRYPRLTFTIGSDLPQNDIRVDVV
                     NATGEIVRTFYRDDVAPDLPNTIRWDGTSNEGKPARNGKYTFRVSPQTPAAPAARKAT
                     ATTTLKLGFTLYGYTFPILGAHDFGGAMGRFGAGRSGHTHQGQDVMAPCRTPLVAARG
                     GKVQYAGWDDGGGNYIVIDGKGTANDFVYMHMVEPALLRAGETVRTGQPIGYVGTTGS
                     SSGCHLHFEIWSGPGWYEGGSPFDPLAELQKWDRYS"
     gene            complement(2606..3061)
                     /locus_tag="VFB52_00950"
     CDS             complement(2606..3061)
                     /locus_tag="VFB52_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZO04930.1"
                     /translation="MATQTKTGIDSAWAEHALETLQGAGHRRGGARTAVVEALAGHDC
                     AVTAIELDDELRRRKPAVARASVYRALEQLEELGLVRRIEVCRGTAGFERVDPAGHHH
                     HHAICRDCGRMVPFEDRSLEQAIHRLSESIDFDVSEHDVVLKGRCEKCS"
     gene            complement(3063..3902)
                     /locus_tag="VFB52_00955"
     CDS             complement(3063..3902)
                     /locus_tag="VFB52_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZO04931.1"
                     /translation="MFEPLTEPFMQRAIAEMTLLGLAGGALGCWIVLHELSYAAESLA
                     HSLFPGLVIAAIAGLPLLLGATPAVILAALAIAVAARLPDVGRDVGIAVVVTTMFGLG
                     VLLALSPDSPPGVEALLFGDILGPSDGDLIAAGALVVLLAVALPLLHGRLLATGFDPE
                     AARVLGVSPALAGAALLVLLAAATVVAVQGLGNLLVVAVFVGPAATARNLSDRVVPMI
                     AISAAVAVFAGLAGLYLSYYAGTAGGASVALAIVAIYLLSLPLGRIRPSTRNFTTAEV
                     YDH"
     gene            complement(3916..4668)
                     /locus_tag="VFB52_00960"
     CDS             complement(3916..4668)
                     /locus_tag="VFB52_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZO04932.1"
                     /translation="MSDAALIEARGLALAYGNGPAVVTGVEFELRAGQRLAMLGPNGG
                     GKTTLLRALLGQLPPREGELVVRASCGSVPQTERSRLDYPVSALEVAAMGALSRQPWW
                     RPPGRAGREQARAALNRVGLGDLADATFGELSGGQRQRVLIARALVQDARVLLMDEPF
                     TGLDRPASERLEALIAELAGEGHAIVMATHDLEQARRWDTVLCVNGRQIAVGPPAAVL
                     DVPVLEATYGGAIVELPDGSGRAVLPAHHHHH"
     gene            complement(4665..6488)
                     /locus_tag="VFB52_00965"
     CDS             complement(4665..6488)
                     /locus_tag="VFB52_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZO04933.1"
                     /translation="MFETFELPFVQRGLVEILILAVPAGLLGTWIVLRRLAFFSHSVG
                     TAAFPGLVLADGLGFAAPLGAFAAALAFTAGNAALGRGRDRGRDSVIALVLVGCLAGG
                     VILASDVFSSGANVETLLFGSLLLVDDADIRLAALAAVVTVAVSALAGRRWLAQGFDP
                     EDAAVSRRERLVLDAALLALIALATTAALSVVGALLVASLFVVPAATARLFTSRMLSW
                     QLASVLLVAAEGTVGLWLSVETDAPPGATIACVAGGVFAVVALGRAVLARAPRGAVAL
                     AGATLLLAAGVAGCGSGGSDTEGKVDVVATTTQIGDFVRAVGGDAVAVDQVLQPNTDP
                     HEYEPRPSDVAAAAGAELVFASGDDLDPWIEEIVEDSGSDAEVVDLSSALPEHLPGEG
                     EEGHEHGEEEHAEDEHEEHAEEDGHHHDSEFDPHWWHDPRNAEAAVREIERSLAAADP
                     AQRALFERNADAYLAQLRRLDREIAGCFDRIPPAERKLVTDHDAFNYFAHRYGIDVVG
                     AVIPSQTTQAQPSAKDVSELIDLIEREGVKAVFPESSISSKVADAIARQTGASSDYEL
                     YGDTLGPEGSPGATYLGMERSNADAMARGFSGGQVGCEAGP"
     gene            complement(6524..7135)
                     /locus_tag="VFB52_00970"
     CDS             complement(6524..7135)
                     /locus_tag="VFB52_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZO04934.1"
                     /translation="MAGKKKRDAGASSIIDPRIVKALSHPTRVQILATLSHRSISPAE
                     FSREYGQPLSDVAYHFRKLEKLECAEVVRTVPVRGSTQHFYAGTRRPLLDDRAWKQLP
                     KSIQPGISSAAIQDLFGRFAEAVRTGTFDARDDRHLSWTPLCVDEEGWEEMVALLWST
                     LEEGNEIGVRAAQRMAQNGNKGFNITFSLAGFESPAEADENES"
     gene            7676..>8015
                     /locus_tag="VFB52_00975"
     CDS             7676..>8015
                     /locus_tag="VFB52_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04935.1"
                     /translation="MSQRPEMIDQRLVKAIAHPLRVRILEVLSDRVSSPNKLSETLET
                     GLTDVAYHTRALDRCGCLQLVDTAQRRGATEHFYKATPDSFLGNPAWRGVPRGILPGV
                     SGATLQTFIDK"
BASE COUNT         1189 a         2780 c         2858 g         1188 t
ORIGIN      
        1 gggaaagaac tcagatctca gtcctaccgc ttcggcgccc tctggcgcaa tcgagaactt
       61 tttagcgctt cgagaactgc ggcttcttac gggccttctt gaagcccgcc ttgcggcgct
      121 ccttgacccg cgcgtcgcgc gtcaggaagc cgcgccgctt gaggtcgccg cgcagctccg
      181 ggtcgatctc ggtcagagcg cgcgcgatgc cgtgccgaac cgcgccggcc tggccgctga
      241 tcccgccgcc gtgcacgcgg atgcggacgt cgacgttgcc ctcgtagccg gagacggtga
      301 gcggctggcg cgccatcgtc tgatgcaggg gccgggggaa gaactcctcg aggctgcgct
      361 tgttgacttc gatcttgccg tcgccgggca gcagggtgac gcgggcgatc gagcgcttgc
      421 gtttgccggt ggcgaggaag cgggcgccgc tctcgatctt ggcgggggcg cgactggcgg
      481 cctcggcctc ggcctcgtcg gcggcggcga gctcggcctc gcggcggagg cgctcctcct
      541 cttcctcggc ttcgacccgg gcgcgctcct cggcgctcag ctcgcggccc tcgggctcga
      601 ccaggatcgg ctccagatcg gcgcccggga tcacgtcttt tttcttctcc tcggccggag
      661 ctttctcggc gaccggctcg gcggcgggct cctcggcggc gggctcgggc tcctcgctcg
      721 cctcggcgac cggctcctcg gcgacgggct cctcggcggg agcctcctca gccggggtct
      781 cctcagcctg cggagcctcc tcggccggct gctcctcggc gggagtctcc tccaccggcg
      841 actcggcctg cggctcctcg ggggcctcgg cggtcggctc ggtgttctcg tccggggtct
      901 ccggagtctg ctcgtcctcg gggatcaagt tagttcgtct ccatcggttt gggctgctgc
      961 gcctggtgcg ggtgatcggg gccggcgtag accttcagct tggtcagctg ctggcgggcg
     1021 aggcggttgc gcgggagcat tcccttgacc gccttgcgga tcacttcctc gggctgccgg
     1081 tcgagcatct cggccagcgt gcgcgacttg atgccgcccg ggtagccgct gtggcgccag
     1141 taggtcttct gcgccagctt gtcgccggtg acgcggatct tctccgcgtt gacgacgacg
     1201 atgaagtcgc cggtgtcgac gtgcggggtg tactcgggct tgcgcttgcc gcgcagagcg
     1261 tcggcgatct gggtcgcgag acggcccagg gtcttgccct cggcgtcgac gacgtaccag
     1321 tcgcgctggc ggttctctgc gttggctacg taggtcttca cggtcatatc gcgctctcag
     1381 tcattaaaaa gccacgcgga cgtaccacgc ggccggggga tgatagccag gttcagcgac
     1441 tggcggccgc cccggggtcc gcgtggtgac ttcaggccgg ttacctatac tgccgcgcca
     1501 gcaaatgcct aatcccatcg ggcaaccgat tgggagtgga ggacccaggg ggatcgaagc
     1561 aggtcccggg ggcgaatcgg gcgaatctcg cccgtagcgc gactctttca gcgcgagccc
     1621 gacagctaac tccgcaggcg gacgaaagag agggcttagg cggtgcgcag cacgaggacc
     1681 cggaccggga aggccggtgc aatcacgatc ttggcgttgt tcgccctgct cgccacctgc
     1741 gctgccgcgg tcgccgccac cggcggcgcc cccaccccga cctcgactcc cccgcccccc
     1801 ggcagcggct ccggcgctcc cgcagccgaa ccgagcgccg gcgcggtcac cctcctcgcc
     1861 gccgaaacgg cgccgcgcaa gtccttctac tacggctacc gctacccgcg gctgaccttc
     1921 acgatcggca gcgacctgcc ccagaacgac atccgcgtcg acgtcgtcaa cgcgaccggc
     1981 gaaatcgtcc gcaccttcta ccgcgacgac gtcgcgcccg acctgccgaa cacgatccgc
     2041 tgggacggga ccagcaacga aggcaagccg gcccgcaacg gcaagtacac cttccgggtc
     2101 agtccccaga ccccggccgc accggcggcg cgcaaggcga ccgcgacgac caccctgaag
     2161 ctcggcttca ccctctacgg ctacaccttc ccgatcctcg gggcccacga cttcgggggc
     2221 gcgatgggcc gcttcggcgc cggccgctcc ggccacaccc accagggtca ggacgtgatg
     2281 gccccctgcc gcacgcccct ggtcgccgcc cgcgggggca aggtccagta cgccggctgg
     2341 gacgacgggg gcggcaacta catcgtgatc gacggcaagg gcaccgccaa cgacttcgtc
     2401 tacatgcaca tggtcgaacc ggccctgctc agggcgggcg aaacggtgcg caccggccag
     2461 ccgatcggct acgtcggcac caccggctcc tccagcggct gccacctcca cttcgagatc
     2521 tggagcggac ccggctggta cgagggcggc tccccctttg accccctcgc cgaactgcag
     2581 aagtgggacc gctacagcta gccggctagc tgcacttctc gcagcggcct ttgaggacca
     2641 cgtcgtgctc ggagacgtcg aagtcgatgc tttcggagag gcggtggatc gcctgctcca
     2701 gggagcggtc ctcgaagggg accatgcggc cgcagtcgcg gcagatcgcg tggtggtgat
     2761 ggtggccggc gggatcgacg cgctcgaagc cggcggtgcc gcggcagacc tcgatccggc
     2821 gcacaaggcc gagctcctcg agctgctcga gcgcccggta gacgctggcg cgggcgaccg
     2881 cgggcttgcg acggcgcagc tcgtcgtcga gttcgatcgc ggtcacggcg cagtcgtggc
     2941 cggccagggc ttcgaccacg gcggtgcggg cgccgccgcg gcggtggccc gcgccttgca
     3001 gggtctcgag cgcgtgctcg gcccaggccg agtcgattcc ggtcttggtc tgcgtcgcca
     3061 tctcagtgat cgtagacctc cgccgtcgtg aaattccgag tgctcgggcg aatcctgccc
     3121 aagggcaggg agagcaggta gatggcgacg atcgccagcg ccaccgaggc accgccggcg
     3181 gtgcccgcgt agtaggagag gtagaggccg gcgaggccgg cgaacaccgc gaccgcggcg
     3241 gagatcgcga tcatcggcac gacccggtcg ctgaggttgc gcgcggtcgc cgccgggccg
     3301 acgaagacgg cgacgacgag caggttgccg aggccctgca ccgcgaccac ggtcgccgcc
     3361 gccagcagga cgagcagcgc cgcgccggcg agggccggcg agacgccgag cacgcgcgcc
     3421 gcctcggggt cgaacccggt cgcgagcagg cggccgtgca gcagcggcag ggcgaccgcg
     3481 agcaggacga cgagggcgcc ggcggcgatc aggtcgccgt cgctgggccc gaggatgtcg
     3541 ccgaacagca acgcctcgac ccccggtggg gagtccggcg agagcgccag caggacgccg
     3601 aggccgaaca tcgtggtcac gacgaccgcg atcccgacgt cgcggccaac gtcgggcagc
     3661 cgcgcggcga ccgcgatcgc cagcgccgcc agaatcaccg ccggggtcgc gccaagcagc
     3721 agcggcaggc cagcgatcgc ggcgatcacc aggcccggga agagcgagtg ggcgagcgac
     3781 tcagccgcgt aggagagctc gtggaggacg atccagcagc cgagcgcgcc gccggccagg
     3841 ccgagcaggg tcatctcggc gatcgccctc tgcatgaacg gttcggtcag cggctcgaac
     3901 atctcagtgg ctcactcagt ggtggtgatg gtgggcgggc aggacggcgc ggccgctgcc
     3961 gtcgggcagc tcgacgatcg cgccgccgta ggtggcttcg agcacgggca cgtcgagcac
     4021 cgcggccggt ggcccgacgg cgatctggcg gccgttgacg cagagcaccg tgtcccagcg
     4081 gcgcgcctgc tcgagatcgt gggtcgccat cacgatcgcg tgcccctccc ccgccagctc
     4141 ggcgatcagc gcttcgaggc gttcggaggc gggccggtcg agaccggtga acggctcgtc
     4201 catcagcagc acccgcgcgt cctggaccag ggcgcgggcg atcaggacgc gctggcgctg
     4261 gccgccggag agctcgccga aggtcgcgtc ggcgagatcg ccgaggccga cccggttaag
     4321 cgcggcgcgc gcctgctcgc ggccggcgcg gccgggggga cgccaccagg gctggcgcga
     4381 gagcgcaccc atcgccgcca cttcgagcgc cgagaccggg tagtcgagcc gcgagcgctc
     4441 ggtctggggc accgagccgc aggaggcgcg gacgacgagc tcgccctcgc gcgggggcaa
     4501 ctggccgagc agggcgcgca gcagcgtcgt cttgccgccg ccgttgggac cgagcatcgc
     4561 cagccgctgg ccggcccgca gctcgaactc gaccccggtg acgaccgcgg ggccgttgcc
     4621 gtaggcgagc gcgaggccgc gcgcctcgat cagggccgcg tcgctcatgg gcctgcttcg
     4681 cacccaacct gcccgccgct gaaaccgcgg gccatcgcgt cggcgttcga gcgctccatc
     4741 ccgaggtagg tggcgccggg cgagccctcg gggccgaggg tgtcgccgta gagctcgtag
     4801 tcagaggagg cgccggtctg gcgcgcgatc gcgtccgcga ccttggagct gatcgagctc
     4861 tccgggaaga ccgccttcac cccttcgcgc tcgatcaggt cgatcagctc gctgacgtcc
     4921 ttggccgagg gctgcgcctg ggtggtctgc gaggggatca cggcgccgac cacgtcgatt
     4981 ccgtagcggt gggcgaagta gttgaaggcg tcatggtcgg tgaccagctt gcgctctgcc
     5041 ggcgggatcc ggtcgaagca gccggcgatc tcgcgatcga ggcggcggag ctgggccaga
     5101 taggcgtcgg cgttgcgctc gaacagcgcc cgctgcgcgg gatcggcggc ggccaggctg
     5161 cgctcgatct cgcgcaccgc ggcctcggcg ttgcgcgggt cgtgccacca gtgcgggtcg
     5221 aactcggagt cgtggtggtg gccgtcctcc tcggcgtgct cctcgtgctc gtcctcggcg
     5281 tgctcctcct ccccgtgctc gtgcccctcc tcgccctcac ctgggaggtg ctcgggcagg
     5341 gcgctgctga ggtcgacgac ctccgcgtcg ctgccgctgt cttcgacgat ctcctcgatc
     5401 caggggtcga ggtcgtcgcc gctggcgaag acgagctcgg cgccggccgc ggcggcgacg
     5461 tcgctgggtc gcggctcgta ctcgtgcggg tcggtgttcg gctgcaggac ctggtcgacg
     5521 gccaccgcgt cgccgccgac cgcgcgtacg aagtcgccga tctgggtggt ggtcgcgacc
     5581 acgtcgacct tgccctcggt gtcggagccg ccgctgccac agccggcgac ccctgcggca
     5641 agcagcagcg tcgctccggc cagggcgacg gcgccgcgcg gagcccgcgc caacacggcc
     5701 cggccgagcg cgaccacggc gaagacgcca ccggcgacac aggcgatcgt cgccccgggg
     5761 ggcgcgtcgg tttcgaccga gagccagagg ccgaccgtgc cctcggcggc gaccaggagc
     5821 acgctggcga gctgccagct cagcatccgg ctggtgaaca ggcgcgcggt cgccgccggg
     5881 acgacgaaca gcgaggcgac cagcagcgcg ccgacgaccg agagcgccgc cgtcgtcgcc
     5941 agcgcgatca gggcgagcag cgcggcgtcg agcacgaggc gctcgcgccg gctgaccgct
     6001 gcgtcctcgg ggtcgaagcc ctgggcgagc cagcggcggc ccgcgagcgc gctgaccgcg
     6061 acagtgacga cggccgccag cgcggccagc cggatgtcgg cgtcgtcgac cagcagcagg
     6121 ctgccgaaga gcagggtctc gacgttggcg ccggagctga agacgtcgct ggcgaggatc
     6181 acgccgccgg ccaggcagcc gaccagaacc agggcgatga cgctgtcgcg gccgcggtcg
     6241 cgtccccgcc ccagggcggc gttaccggcg gtgaaggcga gcgcggcggc gaaggcaccg
     6301 agcggcgcgg cgaagccgag gccgtcggcg aggaccaggc cggggaaggc ggcggtgccg
     6361 accgagtggg agaagaaggc gaggcggcgg agcacgatcc aggtcccgag caggccggcc
     6421 gggaccgcca ggatcaggat ctcgactagg ccgcgctgaa cgaagggaag ttcgaaggtc
     6481 tcgaagattc gaggatcata atgagaatga gtccagtttg tatttaggac tcgttctcgt
     6541 cagcctcggc gggcgactcg aacccggcca gggagaaagt gatgttgaag cctttgttcc
     6601 cattctgggc catccgctgg gctgcgcgca cgccgatctc gttgccttcc tcgagcgtgc
     6661 tccagagcag cgccaccatt tcctcccagc cctcctcgtc gacgcagagc ggggtccagc
     6721 tcaggtggcg atcgtcgcgc gcgtcgaagg tccctgtgcg taccgcttcg gcgaagcggc
     6781 cgaaaaggtc ctggatcgcg gcgctcgaga tccccggctg gatcgacttc ggcagctgct
     6841 tccaggcgcg gtcgtcgagc agggggcggc gcgtgccggc gtagaagtgc tgggtcgagc
     6901 ccctgaccgg gaccgtgcgc acgacttcgg cgcattcgag tttctcgagc ttgcggaagt
     6961 ggtaggcgac gtcggaaagc ggctggccgt actcgcggga gaactcggcg ggactgatcg
     7021 agcggtggct gagcgtggca aggatctgca cccgagtcgg atggctgagc gctttcacga
     7081 tcctgggatc gatgatcgac gacgccccgg cgtctcgctt cttctttcca gccactgcct
     7141 tcccccctat tagtcaacag tttataagcg atcaccctaa accgttttcc cgccgcccac
     7201 gcaccatttt gcggcccagg cgtaaaccgc ttgaatctcg tgaagtaata gatgcggtct
     7261 cccgtcggcg ccggagtacc ggcgtgtcat gacgtcctcg aaagccaacc tgagggggga
     7321 ggtgaggaaa ttgaggatgg agaccatgtc ctgcgccatt ccggcgtgct cagtgacgaa
     7381 gtccggctga aatagccact gagctcgccg acgtggccgt cccggctgac tacccaggga
     7441 gggagccggg gcggccgcgg gcgcctggtc tccctcagca aacgattggc ctcaccaacg
     7501 gacgagtgac ctattgatca aaatattttg ttcctgtttg gggattctta ccgaatacat
     7561 ctgatccaaa ccattagtaa ggacgtattt gagtaagtaa cgaacgtcgg cgtacagccg
     7621 acgggatcca catcggtggt ggcaacgcct ccgatcccca aaaaaggagc cactaatgag
     7681 tcagcgaccg gaaatgatcg accaacgcct tgtgaaggcg atcgcgcatc ccctgcgtgt
     7741 gcgcatcctc gaggtgttga gcgatcgagt ttcgagcccc aacaaactct ccgagacact
     7801 ggagaccggc ctcaccgacg tcgcgtacca cacgcgcgcg ctcgatcgct gcggctgcct
     7861 gcagctcgtc gacaccgccc agcggcgcgg tgccaccgag cacttctaca aggcgacgcc
     7921 ggactcgttc ctcggcaacc cggcctggcg tggcgttccc cgcggcatcc tccccggtgt
     7981 ctccggagcc accctgcaga ccttcatcga caagg
//
LOCUS       DASRIG010000037         7817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3141073, whole genome shotgun sequence.
ACCESSION   DASRIG010000037 DASRIG010000000
VERSION     DASRIG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141073"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VFB52_00980"
     CDS             complement(<1..1161)
                     /locus_tag="VFB52_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO04936.1"
                     /translation="MDLVTKALRAGEGRKFKAYQKRAEAINRYEPEMEALEDDELRTE
                     ADSLRERARNGESLDELLPEAFALVREASRRTTGQRHYDVQLVGGMVLHDGSIAEMKT
                     GEGKTLTATLPVFLNTLAGGSVHVVTVNDYLARRDSEWMTPIYEALGVTVAAVQDADD
                     HATRQRKYACDVTYGTNSEFGFDYLRDNMSGSLENCVQRSHDFAIVDEVDNILIDEAR
                     TPLIISGQPEQAAQTYYTFARLAKQMVGVPAKQKLKSLGESKDTTEADYDYEYDEKHK
                     TVAPTEGGVKKAEEFLGVDNLYLGEHGSLVNHLVQSLKAESLYKKDDDYAVIDGEVMI
                     IDEFTGRILEGRRWSEGLHQAVEAKEGVSIREENQTLATITLQNYFRLYDKLS"
     gene            1253..1606
                     /gene="raiA"
                     /locus_tag="VFB52_00985"
     CDS             1253..1606
                     /gene="raiA"
                     /locus_tag="VFB52_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZO04937.1"
                     /translation="MRIEIRGRNVEMTEDLREMVRKRFQRLGQQVSELATLEVVLSEE
                     RNPANPNGQVAEATFYLKGVTLHARECSPEMTHSVHELAEDVRRQVKKHRDLRRKRRQ
                     TRKLVSRMRGRPVAT"
     gene            complement(1628..2251)
                     /locus_tag="VFB52_00990"
     CDS             complement(1628..2251)
                     /locus_tag="VFB52_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZO04938.1"
                     /translation="MDALRALTAVVAPPLCAACGRSCRSEAVLCTRCTRRLAAAEPLL
                     GKGPPGLDRAWSSAAHEGVARDLVAALKFRRLLPVADLMAERIHWLAPAHMLSGAIVP
                     VPPAPLRTRRRGFDPTGEIAAALAELLETSVEPCLARRGGARQVGRRRAERVAHPPRV
                     HARCEVPRSVLLVDDVLTTGTTLTACAKALRAAGAARVVAVTFARRL"
     gene            complement(2325..3323)
                     /locus_tag="VFB52_00995"
     CDS             complement(2325..3323)
                     /locus_tag="VFB52_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZO04939.1"
                     /translation="MVLAAGLGTRLRPITYAMPKPMVPVANRPVMEHILRLLARHGFT
                     ETIANLHWFPDLIRDYFGDGSASGVELSYSYEEQLLGTSGGVRKAADFLGDSFLVISG
                     DALTDLDLGAMREFHESHDGIATLATKRVADTSQFGVAITGADGRIQGFQEKPDPAEA
                     LSDLANCGIYMFRKEIFDFFPAPGTSKAAGPGDPDGFADWAMDVFPRLLDGDVPFYSH
                     ETEAYWDDIGNLEELRAATRDALTGDVEVELEGELVEGFRSGTPDGEEGKLLGPVLIG
                     PGCELADDVRIDGPSVLGPGVRVGAGSRLREVIALPGVEIAAGSVLVGAIVGARAA"
     gene            complement(3360..5366)
                     /locus_tag="VFB52_01000"
     CDS             complement(3360..5366)
                     /locus_tag="VFB52_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZO04940.1"
                     /translation="MTTKNFCVLTPHSIRFKALALLFLSATLLALGHGRAEAANTLQV
                     APTGSDSGNCVSAACATIGYAVGEASADDTIQIAAGDYDEQIEITVPGLTLQGAGATT
                     VIGPEGDSVVEQTEITLADGVDGVTLKDLAVVSAAQGKPAILIEGPEAIDDTTLDGVA
                     VSGIGPSTPPTTVGNGLEIKAPGSGWKVLDSSFSFHYLALVVFGDLEDFTIEGSSFND
                     NRAGFYVERTLPLTSSSTGEIDGLQMSDTTFDDEEYVPLYFEGLSNALIEGVTVTDTG
                     VGVSPLPAGGRAVVINLKAGAGENIEIADSTFTGSVNEGILVQARGWAGDSGTYVAAP
                     ASLDGFELRDSTVTGNGGPGVIVNNQSTLTGVSIHNNRIVANGTAGFNVTAPVNGVFA
                     RNTEGGAPPVPTAENWFACNESPAAGTCAAVNAPVQAPTWLVLGVDTSFETLGVGEQT
                     AVVAAIESSAGGDPLTPLPGSPEAQFSSALGSFEPAAEPLLDGVASPTFIAGQTPGTG
                     VITVEVDGEEASTPIAVHPPDKVDPPAPPAPPAPLPPAPEPPTIEPPANKGPTTVPNS
                     GTVTVATVGCASESCTVKTQGTPSVKIGGKSYKVTVKVPKNLGAGDSGKVKVVLPKKV
                     REALAEEGKGKLKLKLKVTGSDGSTKTVTVTIVLKGKGAKAKGG"
     gene            complement(5478..6470)
                     /locus_tag="VFB52_01005"
     CDS             complement(5478..6470)
                     /locus_tag="VFB52_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HZO04941.1"
                     /translation="MIDDVLGIPDQLRDALWRVETARLEAAESAGLIVCGMGGSAIGA
                     DLAAAALGDRATRQLVTVRGYGLPSWATPEWTVLCSSYSGGTEETLACFEAATALGAR
                     RIVASTGGALVDSAREDGVPVVGLPGIFQPRAAVAYMFTIAAEVAALAGVAPRIHTEI
                     DAAAAFLTEQLETLKFQAAEVAGLLEGTAPIVYGSDLTAPVARRWKTQANENAKLPAF
                     YSELPEADHNELCGWNEAAAEHRLAAVFLEDRDQHPRIARRFELTAEIVDGSGAKAVR
                     IETAGETRTERLFWATMLGDLVSLELAAQRGVDPLPVEAIEGFKRSMGDPAEVE"
     gene            complement(6467..7465)
                     /locus_tag="VFB52_01010"
     CDS             complement(6467..7465)
                     /locus_tag="VFB52_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZO04942.1"
                     /translation="MQAIVLVGGEGTRLRPLTNDVPKPALTLVDRPFLTYMIEWLAGH
                     GVTEAVFACGFLPDVLRETVGEGEHAGVKLSYVAEPDRRGTGGAIRFAADSLGERLED
                     RFLALNGDVLTDLDLTALQGAHAASGGAGTLALYPVEDSSAYGLVSCDGEGAVVEFLE
                     KSGEAVPGEINAGAYVLERSVMDLIPPGREVSIEREVFPKLIGNGLYGKRLDGYWMDI
                     GTPERYLEASWDILEGRVATRVEPTAPGLMVGADTDVAAGATLGPRAVVGPGCRIEAG
                     ASVRESILLDGCVVAEGASVSGSILAPGVVVEPGVTLSDAVIGRRERVSAEQAVES"
     gene            complement(7465..>7817)
                     /locus_tag="VFB52_01015"
     CDS             complement(7465..>7817)
                     /locus_tag="VFB52_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZO04943.1"
                     /translation="MREASSLVLAARLQGEGAEVVAYDPVAAERAAELLDSVEMADSA
                     LAALDGADAVVLVTEWREFAELDWGEAAARMPRPLIVDGRNFLDAEKLVAAGFQYEGI
                     GKIAADSPAPAGQS"
BASE COUNT         1122 a         2647 c         2830 g         1218 t
ORIGIN      
        1 ggagagcttg tcgtagaggc ggaagtagtt ctgcagggtg atcgtcgcca gggtctggtt
       61 ctcctcgcgg atcgagacgc cctccttggc ctcgaccgcc tggtgcagac cctccgacca
      121 gcgccggccc tcgaggatgc ggccggtgaa ctcgtcgatg atcatcactt cgccgtcgat
      181 caccgcgtag tcgtcgtcct tcttgtagag cgactccgcc ttcagcgact ggaccaggtg
      241 gttgaccagg ctgccgtgct cgccgaggta gaggttgtcg acgccgagga actcctcggc
      301 ttttttgacc ccgccctcgg tcggcgccac cgtcttgtgc ttctcgtcgt actcgtagtc
      361 gtagtcggct tcggtggtgt ccttcgattc gccgagcgat ttcagtttct gtttcgccgg
      421 cacgccgacc atctgtttgg ccaggcgggc gaacgtgtaa taggtctggg ccgcctgttc
      481 gggctggccg gagatgatca acggcgtccg cgcctcgtcg atcaggatgt tgtcgacctc
      541 gtccacgatc gcgaagtcgt ggctgcgctg gacgcagttc tcgagcgagc ccgacatgtt
      601 gtcgcgcagg tagtcgaagc cgaactccga gttggtcccg taagtgacgt cgcaggcgta
      661 tttgcgctgg cgggtggcgt ggtcgtcggc gtcctggacg gcggcgacgg tgaccccgag
      721 cgcctcgtag atcggcgtca tccattccga gtcgcgacgg gcgaggtagt cgttgacggt
      781 gaccacgtgg acgctgccgc cggccagcgt gttgaggaac acgggcaggg tcgcggtcag
      841 ggtcttgccc tcgccggtct tcatctcggc gatcgagccg tcgtggagga ccatgccgcc
      901 gaccagctgc acgtcgtagt ggcgctgacc ggtggtccgg cgcgaggcct cgcggaccag
      961 cgcgaacgcc tccggcagca gctcatcgag ggactcgccg ttgcgagcgc gctcgcgcag
     1021 ggagtcggcc tccgtgcgca gctcatcgtc ctcgagcgct tccatctcgg gctcgtagcg
     1081 gttaatcgcc tcagcgcgct tctggtaggc cttgaacttc cgaccctccc ccgcgcgcaa
     1141 ggccttcgtg acgaggtcca ttgcgggcat tgctcaaggg tagcgatgca ggccttgctc
     1201 cgagccgccc ccgggcgtag catcctcaca aggaacccac aaggaggcac tcatgcggat
     1261 tgagattcgg ggccgaaacg tcgagatgac ggaagacctg cgtgagatgg tgaggaagag
     1321 attccagcgc ctgggccagc aagtgtctga gctggccacc cttgaggtgg tgctttcgga
     1381 agagcggaac cccgcaaacc cgaacggcca ggtcgccgag gcgaccttct acctgaaggg
     1441 cgtgacgctg catgctcgcg agtgctcacc ggagatgacc cacagcgtgc acgagctggc
     1501 agaggacgtg cggcgccagg tcaagaagca ccgggacctg cgacgcaagc ggcgccagac
     1561 gcgcaagctg gtcagccgca tgcgggggag gccggtagct acctgagctt ttaacgggcc
     1621 cgccgcctca gagccgacgg gcgaaagtca cggcgacaac gcgtgcggct ccggccgcac
     1681 gcagtgcttt ggcgcaggcg gtgagggtgg tgccggtggt gagcacgtcg tcgacgagga
     1741 ggacgctgcg ggggacctcg cagcgggcgt ggacgcgcgg cggatgggcg acgcgttcgg
     1801 cgcggcggcg accgacctgc ctggcgccgc cgcggcgggc gaggcagggc tcgaccgagg
     1861 tctcgagcag ctccgccagc gcggcggcga tctcgccggt cgggtcgaag ccgcggcggc
     1921 gggtgcgcag cggagccggc ggcaccggga cgatcgcgcc gctgagcatg tgagccggag
     1981 ccagccagtg aatccgctcg gccatcaggt ccgcgaccgg cagcaggcgg cggaacttga
     2041 gcgcggcgac gaggtcgcgg gcgacgccct cgtgggccgc cgaggaccag gcgcggtcga
     2101 gccccggcgg gcccttgccg agcagcggct cggcggctgc caggcggcgc gtgcagcgcg
     2161 tacagaggac tgcctcggag cgacaggagc ggccgcaggc ggcgcagagc ggcggggcga
     2221 cgacggcggt gagggctcgg agcgcgtcca cgcgccgatc ctcggcgccg tcggcgcacg
     2281 catggcgcgc agaaggtgaa ttctctgcgc cgaaagggtc gcgactatgc ggcgcgggcg
     2341 ccgacgatcg cgccgaccag aaccgagccg gcggcgatct cgacgccggg cagggcgatc
     2401 acttcgcgca ggcggctgcc ggcgccgacc cgcacacccg ggccgagcac cgaggggccg
     2461 tcgatgcgga cgtcgtcggc gagctcgcag ccggggccga tcaggaccgg gccgagcagc
     2521 ttgccctcct cgccgtcagg cgtgccgctg cggaagccct cgaccagctc gccctccagt
     2581 tcgacctcga cgtccccggt gagcgcgtcg cgggtcgccg cccgcagctc ctcgaggttg
     2641 ccgatgtcgt cccagtaggc ctcggtctcg tgcgagtaga agggcacgtc gccgtcgagc
     2701 aggcgcggga acacgtccat cgcccagtca gcaaagccgt cggggtcgcc ggggccggcc
     2761 gctttgctgg tcccgggcgc cgggaagaag tcgaagatct ccttgcggaa catgtagatc
     2821 ccgcaatttg cgaggtcgga gagcgcttcg gccgggtctg gcttctcctg gaagccctgg
     2881 atccgcccgt cggcaccggt gatcgcgacg ccgaactggc tggtgtcggc gacccgcttg
     2941 gtcgccaggg tcgcgatccc gtcgtgggac tcgtggaact cccgcatcgc gcccaggtcg
     3001 aggtcggtga gcgcgtcgcc ggagatcacc aggaacgagt cgccgaggaa gtcggcggcc
     3061 ttgcgcaccc cgcccgaggt gccgagcagc tgctcctcgt aggagtagga gagctcgacg
     3121 ccactggccg aaccgtcacc gaagtagtcg cggatcaggt ccgggaacca gtgcaggttg
     3181 gcgatcgtct cggtgaagcc gtgccgggcc agcagccgca ggatgtgctc catcaccggc
     3241 cggttggcga ccggcaccat cggcttcggc atcgcgtagg tgatcgggcg aaggcgggtt
     3301 ccgaggccag ccgcgaggac cattgctctc acgcgactga ccgtatcgga cccgcggcgt
     3361 cagccgccct tcgccttcgc ccccttgccc ttgagcacga tcgtcaccgt caccgtcttg
     3421 gtcgagccgt cggatccggt cactttcagc ttcagcttga gcttgccctt gccttcctcg
     3481 gcgagcgctt cccgcacctt cttcggcaag acgaccttga ccttgcccga gtcgccggcg
     3541 ccgaggtttt tcggcacctt cacggtcacc ttgtaggact tgccgccgat cttcacgctc
     3601 ggggtgccct gcgttttcac cgtgcaggac tccgacgcgc agccgacggt cgcgaccgtg
     3661 accgtgccgc tgttcggcac cgtggtcggg cctttgttgg ccggcggttc gatcgtcggc
     3721 ggttccgggg ccggtggcag cggcgcgggc ggtgccggag gagccggcgg gtcgactttg
     3781 tcgggcgggt gcacggcgat cggcgtgctc gcttcctcgc cgtcgacctc gaccgtgatc
     3841 acacccgtgc ccggcgtctg accggcgatg aaggtcggcg aggcgacccc gtcgagcagc
     3901 ggttcggcgg ccggttcgaa gctgccgagg gccgagctga actgcgcttc aggggagccg
     3961 gggagcggcg tcagcggatc gccgccggcg ctgctttcga tcgccgcgac gaccgcggtc
     4021 tgctcgccga ctccgagcgt ctcgaacgaa gtgtcgacgc ccagcaccag ccaggtcggg
     4081 gcctgaaccg gggcgttgac ggcggcgcag gtaccggcgg cgggggactc gttacaggcg
     4141 aaccagttct cggcggtggg aaccgggggc gccccacctt cggtgttgcg ggcgaagacg
     4201 ccgttcacgg gagcggtgac gttgaagccc gcggtgccgt tggcgacgat ccggttgttg
     4261 tggatcgaga ccccggtgag ggtcgactgg ttgttgacga tcacgccggg accgccgttt
     4321 ccggtcacgg tcgagtcgcg cagctcgaac ccgtcgagcg acgccggtgc ggcgacgtag
     4381 gtgccgctgt cgccggccca gccgcgggcc tggacgagga tgccctcgtt gaccgagccg
     4441 gtgaaggtcg agtcggcgat ctcgatgttt tcgccggccc cggccttgag gttgatcacg
     4501 acagcgcggc cgccggccgg cagcgggctg accccgacgc cggtgtcggt cacggtgacg
     4561 ccttcgatca gcgcgttgct gaggccctcg aagtagagcg ggacgtactc ttcgtcgtcg
     4621 aaagtcgtgt cggacatctg caggccgtcg atttcgccgg tcgagctcga cgtcagcggc
     4681 agcgtccgtt cgacgtagaa gccggcccgg ttgtcgttga agctcgaccc ttcgatcgtg
     4741 aagtcctcga ggtcgccgaa gacaacgagc gcgaggtagt ggaaggagaa ggagctgtcg
     4801 aggactttcc agccgctgcc cggggctttg atttcgagcc cgttgccgac agtggtcggc
     4861 ggcgtgctcg ggccgatccc ggagacggcg accccgtcga gggtcgtgtc gtcgatcgct
     4921 tccggccctt cgatcaggat cgccggtttg ccctgggcgg cggagaccac cgcgaggtct
     4981 ttgagggtga cgccgtcgac gccgtcggcg agcgtgatct cggtctgctc gacgacgctg
     5041 tcgccctcgg gcccgatcac ggtggtggca ccggcgccct gcagggtcag cccgggcacg
     5101 gtgatctcga tctgctcgtc gtagtcgccg gcggcgatct ggatcgtgtc gtcggcagag
     5161 gcttcgccga ccgcgtagcc gatcgtcgcg caggcggcgc tgacgcagtt gccgctgtcg
     5221 ctacccgtcg gcgcgacctg aagcgtgttg gctgcctcag cgcggccgtg gcccagggcg
     5281 agcagggtcg cgctcaggaa gagcagcgcg agggccttga aacggatgct gtggggggtt
     5341 agaacgcaaa agtttttagt ggtcatgcat gccgagttcg acctaattca ggcaaaactc
     5401 aaacgaaact cagacaaact ggacaggcgt ccagaggcac tggaccaagg ctcgcaaatc
     5461 tgctgttgcg aaccggctta ctcgacctcg gcggggtccc ccatcgagcg cttgaagccc
     5521 tcgatcgcct cgaccgggag cgggtcgacg ccgcgctggg cggccagctc gagcgagacg
     5581 aggtcgccca gcatcgtcgc ccagaagagc cgctcggtgc gggtctcgcc ggcggtctcg
     5641 atccgcaccg ccttcgcccc ggagccatcg acgatctcgg cggtgagctc gaagcggcgc
     5701 gcgatccgcg ggtgctggtc gcggtcctcg aggaagacgg cggcgagacg gtgctcggcg
     5761 gcggcctcgt tccagccgca gagctcgttg tggtcggcct cgggcagctc cgagtagaag
     5821 gcgggcagct tcgcgttctc gtttgcctgc gtcttccagc ggcgggccac cggcgcggtc
     5881 aggtcggagc cgtagacgat cggggcggtg ccctcgagca gcccagcgac ctcggccgcc
     5941 tggaacttca gcgtctccag ctgctcggtc aggaaggcag cggcggcgtc gatctcggtg
     6001 tggatgcggg gcgcgacccc ggccagggcg gcgacctcgg cggcgatcgt gaacatgtag
     6061 gcgaccgcgg cgcggggctg gaagatgccc gggaggccga ccacggggac gccgtcctcg
     6121 cgggcgctgt cgaccagggc gccgccggtg ctggcgacga tccggcgggc gccgagcgcg
     6181 gtcgcggcct caaagcaggc gagcgtctcc tcggtccccc ccgagtagct cgagcagagc
     6241 acggtccact ccggcgtcgc ccagctgggc agcccgtagc cgcgcacggt gaccagctgc
     6301 cgggtcgcgc ggtcgccaag cgcggcggcg gcgaggtcgg cgccgatcgc cgagccgccc
     6361 atcccgcaga cgatcaggcc ggccgactct gccgcctcca agcgcgcggt ttcgacccgc
     6421 cagagcgcgt cgcggagctg gtcggggatg ccgaggacgt cgtcgatcac gactcgactg
     6481 cctgctcggc actaacccgc tcgcggcggc cgatcacggc gtcggagaga gtgactccgg
     6541 gctcgacgac gacccctggc gcgaggatcg acccgctgac gctcgccccc tccgccacca
     6601 cgcaaccgtc aagcaggatc gactcgcgca cgctcgcgcc cgcctcgatc ctgcagccgg
     6661 ggccgacgac cgcccgcggt cccagcgtcg cccctgcggc gacgtcggtg tcggcgccaa
     6721 ccatgaggcc cggcgcggtc ggctcgaccc gggtcgcgac ccggccctcg aggatgtccc
     6781 agctcgcttc caggtagcgc tcgggggtgc cgatgtccat ccagtaaccg tcgaggcgct
     6841 tgccgtagag gccgttgccg atcagcttcg ggaagacttc gcgctcgatc gagacctcgc
     6901 gtccgggcgg gatcaggtcc atcaccgagc gctcgagcac gtaggcgccg gcgttgatct
     6961 caccgggcac ggcctcaccg ctcttttcga ggaactcgac cacggcgccc tcgccgtcgc
     7021 agctgaccag cccgtaggcg gaggagtcct cgaccgggta gagggcgagg gtcccggcgc
     7081 cgccgctggc cgcgtgggcc ccctgcaagg cggtcaggtc gaggtcggtg agcacgtcgc
     7141 cgttgagcgc caggaagcgg tcctcgagcc gctcgccgag cgagtcggcg gcgaagcgga
     7201 tcgcgccgcc ggtgccgcgg cggtcgggct cggcgacata gctgagcttg accccggcgt
     7261 gctcaccctc gccgaccgtc tcgcgcagga cgtcgggcag gaagccgcag gcgaagaccg
     7321 cctcggtcac cccgtggccg gccagccact cgatcatgta ggtgaggaag ggccggtcga
     7381 ccagggtcag cgccggcttc ggcacgtcgt tggtcagtgg ccgcagccgc gtcccttctc
     7441 cccccaccag aacaattgcc tgcatcagct ctggcctgcc ggcgccggcg agtcggccgc
     7501 gatcttgccg atcccctcgt actggaagcc ggcggcaacc agcttctcgg cgtcgaggaa
     7561 gttgcgaccg tcgacgatca gcggccgcgg catccgcgcc gccgcctcgc cccagtcgag
     7621 ctcggcgaac tcacgccact cggtgaccag gaccacggcg tcggcgccgt ccagcgccgc
     7681 cagcgccgag tcggccatct cgaccgaatc gagcagctcg gcggcgcgct cggcggcgac
     7741 cgggtcgtag gcgacgacct cggccccctc accctgcagc cgcgccgcca ggaccaggct
     7801 cgaggcctcg cgcatgt
//
LOCUS       DASRIG010000038         6883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_539795, whole genome shotgun sequence.
ACCESSION   DASRIG010000038 DASRIG010000000
VERSION     DASRIG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539795"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFB52_01020"
     CDS             <1..676
                     /locus_tag="VFB52_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO04944.1"
                     /translation="LEEYELLKKKGKPFFPYAVLKDSAMAVIVVAVIIAMSLILGAEQ
                     GPKADPTSTTYVPRPEWYFFFLFELLRVIKPPELVPIATIGIPTICMVLLLLLPFYDR
                     GPERRPERRPIATTAGIITIIAMGYLTFLGATAGSPTEIDMAVAPQYEAGKEVVAGSG
                     CLACHKIGENGNHGPGPDLTHIASRIPKAAIIRSVEIGPGIMPSFRDLPPKKLEEMAA
                     FLSTLD"
     gene            721..1449
                     /gene="ubiE"
                     /locus_tag="VFB52_01025"
     CDS             721..1449
                     /gene="ubiE"
                     /locus_tag="VFB52_01025"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZO04945.1"
                     /translation="MDTINDNRSSPDFAGQVNRMFDKVAGQYDVLNSVMTAGLHHRWR
                     ERAAQRAGLGPGDSALDVCCGTGDLALELAGRVTPGGNVVGCDFSEPMLDLAREKAAA
                     RGASGVRFEWADALELPYGAERFDAVTVGFGVRNLADLDRGLREMARVLKPGGRAVIL
                     EITQPTRPPLSTFFSLWFDRVVPLLGAFSDDSDAYSYLPESVRSFPSPRGLAEKMDGA
                     GFEQIRYTVLAGGIIAIHSGAKAS"
     gene            1457..2422
                     /locus_tag="VFB52_01030"
     CDS             1457..2422
                     /locus_tag="VFB52_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZO04946.1"
                     /translation="MSQGSSVPPPVTAVLDASSRWLPARMGEVEDQLRGALGDHGELL
                     GADAGATLAAGGKRLRPMLVLLCAGEDGGAEAVRAATAIELIHMATLVHDDVLDEAPL
                     RRGVPTVAATSGRARAEATGDLLFSRAFALLADAGDQRSVALLADASVALAQGELAQR
                     QDAFDLAIDEARYLHRCRLKTARLFECACLLGRDDEHLRAFGTDIGLAFQLLDDVLDV
                     SGPPERTGKARGTDLLDGTITLPLIEAAAADPAIREADLHSLDTESAEALCDRIAATG
                     ALDRVRSRALELVADGKRALERAAFTPEQRQLLALVADGVVQRYS"
     gene            complement(2427..3188)
                     /locus_tag="VFB52_01035"
     CDS             complement(2427..3188)
                     /locus_tag="VFB52_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO04947.1"
                     /translation="MGKLDGTVALVTGASSGIGEATARALAAEGAAVAIAARRADRLQ
                     TLGGEIADAGGTALPIEVDVSEREQAEAAVAKTVAELGRLDVLVNNAGVMLLGPINGA
                     PVEEWERMIEVNLLGLLYTSRAALPHLLAGAEGEPRGVADLINISSVAGRIARKGSGV
                     YNATKHGVGAFSESLRQEVTGRQVRISLVEPGAVDTELQSHNREEIQEQLAKRFASME
                     IMQAGDIADAIAYIVTRPAHVAINELLIRPTGQEG"
     gene            complement(3227..3559)
                     /locus_tag="VFB52_01040"
     CDS             complement(3227..3559)
                     /locus_tag="VFB52_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04948.1"
                     /translation="MREPARNKTKQRKDLHICPECGSDLVQPTFWEQAGDRAHWRVWR
                     RCPECEWFCQGVHGEDEIDAFDEQLDIGAHTLADELRALEHANMSDMADAFVAALHAD
                     LIGADDFT"
     gene            3700..3954
                     /locus_tag="VFB52_01045"
     CDS             3700..3954
                     /locus_tag="VFB52_01045"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZO04949.1"
                     /translation="MFSNIGPLEIGIVLIIALIVFGPKRLPELGSSLGRGIREFKETV
                     TGDRPDDDDDEPDDNVKALSASTSTAAEAAEPEVASDKRG"
     gene            3968..4732
                     /gene="tatC"
                     /locus_tag="VFB52_01050"
     CDS             3968..4732
                     /gene="tatC"
                     /locus_tag="VFB52_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZO04950.1"
                     /translation="MARRVRAISHEDRLSLVEHLDELRGRLIVCIIALATAFALCMWQ
                     NNLLLDIAQGPLPDDRDQLITFGVTEPFTTTLTVVTYGALVLTLPVLLWQLYAYVLPA
                     FSPREKRAVLPILILFPILFLAGLAFAYWVVMPAAVKFLLSFNDSQFNIQVRARDYYS
                     FFGMAMLAGGLVFQMPLVILAITRLGIVKVQQLTSNRRYAYLVIAVLAAALPGVDPIS
                     MLIEMVPLLILFELSIVLAKVFDRRRAAGGVAEPST"
     gene            4751..5605
                     /locus_tag="VFB52_01055"
     CDS             4751..5605
                     /locus_tag="VFB52_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04951.1"
                     /translation="MAKKGGNKDEHKMLFDLRGKRRNVVKVVYAILAVLMGLSLLLIA
                     GPLPFGDIFGAEDAQEVAREQAEEQQQRIEVKLNKDPEDPQLLLTLTRAQIQAGGQLV
                     EEVAPGQLALTVEGRQEYEKAASTWEEYLAATDEPSPALAQQMANTFLTLAQTSPSGP
                     EAETNINAWAEAQKIFAKERPSLGSLSTLALYLPFTFDYAAAKQAANEAKKFATSKFQ
                     REQIDNQLEEAEKNARAFQKSLKEEEQLNQQLEESQSGGGAEGGGGEGDGEGFTNPFN
                     VGGATSSE"
     gene            complement(5614..5687)
                     /locus_tag="VFB52_01060"
     tRNA            complement(5614..5687)
                     /locus_tag="VFB52_01060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5651..5653),aa:Lys,seq:ctt)
     gene            complement(5729..6508)
                     /locus_tag="VFB52_01065"
     CDS             complement(5729..6508)
                     /locus_tag="VFB52_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04952.1"
                     /translation="MSRGRLKRLTIAAVALTALAVVAGASGRGALVEVNNLVLRADGS
                     FEPRQLPRSRFVPLEFEGYARIASKDGTRPVPLRHLVVDFDHDGRLSVRGLPTCPPER
                     IAQATTAEARQACRSAQVGKGTVKGVISLSTGLVEVSSPLTIFNGTPLGGRPTAILHA
                     HFTEPGTQTFAIVVPIERRRGEFAYRATIEIPPLAAGLGSLTAVEVELGRRYRFGGKQ
                     RSYTSARCSDNILRTRGRFSFEDGTVIDGAVSKYCHILPGS"
     gene            complement(6490..>6883)
                     /locus_tag="VFB52_01070"
     CDS             complement(6490..>6883)
                     /locus_tag="VFB52_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04953.1"
                     /translation="NLPGQPQQKVSSAVSFFNGPKLGGQPSLIGHAYETVPAKKTLLV
                     PIAVEKVNKGRYGYHVEVELPQIADGYGVPTLAEATIGRTWNRGGRKVGYLNASCSGG
                     RLQVKGKLEFENGDLFPALLTSPCRVVG"
BASE COUNT         1109 a         2384 c         2321 g         1069 t
ORIGIN      
        1 cctcgaggag tacgagcttc taaagaagaa ggggaagccg ttcttcccct acgcggtgct
       61 caaggactcg gcgatggcgg tgatcgtcgt cgcggtgatc atcgcgatgt cgctgatcct
      121 cggtgccgag cagggcccga aggcggatcc gacctcgacc acctacgtgc cgcgccccga
      181 gtggtacttc ttcttcctct tcgagctctt gcgcgtgatc aagccgccgg agctggtccc
      241 gatcgcgacg atcggcatcc cgaccatctg catggtcctg ctgctcctgc tgccgttcta
      301 cgaccgcggt ccggagcgcc gcccggagcg ccgcccgatc gcgaccacgg ccggcatcat
      361 cacgatcatc gcgatgggct acctgacctt cctcggcgcc accgccggct cgccgaccga
      421 gatcgacatg gcggtcgcgc cgcagtacga ggccggcaag gaggtcgtcg ccggctccgg
      481 ctgcctcgcc tgccacaaga tcggcgagaa cggcaaccac gggccgggac ccgatctcac
      541 ccacatcgct tcgcggattc cgaaagccgc gatcatccgc tcggtcgaaa tcggcccggg
      601 catcatgccc tcgttcaggg atctgccgcc gaagaagttg gaagagatgg cggccttcct
      661 gtcaacgctt gactagggtc gcgcgcgccg cgggcggctg gcctgccacg atgtcctgag
      721 atggacacga tcaacgacaa ccgctcctcg cccgacttcg ccggtcaggt caaccggatg
      781 ttcgacaagg tcgccgggca gtacgacgtg ctcaactcgg tgatgacggc gggcctgcac
      841 caccgctggc gcgagcgggc ggcgcagcgg gccgggctcg ggcccggcga ctcggccctc
      901 gacgtctgct gcgggaccgg cgacctggcg ctggagctgg ccggccgggt cacccccggc
      961 ggcaacgtgg tcggctgcga cttctccgag ccgatgctcg acctcgcccg cgagaaggca
     1021 gccgcgcgcg gcgccagcgg ggtccgcttc gagtgggccg acgcgctcga gctgccctac
     1081 ggggccgagc gcttcgacgc ggtcacggtc ggcttcgggg tccgcaacct cgccgacctc
     1141 gaccgcggtc tgcgcgagat ggcgcgggtg ctgaagccag ggggtcgggc cgtgatcctc
     1201 gagatcaccc agccgacgcg ccccccgctt tcgaccttct tctcgctctg gttcgaccga
     1261 gtcgtgccgc tgctcggcgc cttctccgac gattccgacg cctactccta cctgcccgag
     1321 tcggtgcgca gtttcccgag cccgcgcggg ctggccgaga agatggacgg ggccgggttc
     1381 gagcagatcc gctacacggt cctggccggc gggatcatcg cgatccacag cggcgccaag
     1441 gcgtcctgat cccgggatga gccagggctc ctcggtaccg ccgccggtga cggcggtgct
     1501 cgacgcctcc agccgctggc tgccggcgcg gatgggcgag gtcgaggacc agctccgggg
     1561 agcgcttggc gaccacggcg agctgctcgg tgctgacgcg ggcgcgacgc tggctgccgg
     1621 cggcaagcgg ctgcggccga tgctggttct gctctgcgcc ggcgaggacg gcggcgcaga
     1681 ggcggtccgc gccgcgaccg cgatcgagct gatccacatg gcgacgctgg ttcacgacga
     1741 cgtcctcgac gaggcaccgc tgcgccgcgg cgtgcccacg gtcgccgcca cctccggccg
     1801 cgcccgcgcg gaagcaaccg gcgacctgct cttctcgcgg gccttcgccc tgctcgccga
     1861 tgccggcgat cagcgctcgg tcgccctgct cgccgacgcc tcggtggcgc tcgcccaggg
     1921 tgagctcgcg cagcgccagg acgccttcga cctcgcgatc gacgaggcgc gctacctgca
     1981 ccgctgccgg ctgaagaccg cgcgcctgtt cgagtgcgcc tgtctgctcg gccgcgacga
     2041 cgagcacctg cgcgccttcg gaacagacat cgggctcgcc ttccagctgc tcgacgacgt
     2101 gctcgacgtc agcgggccgc ccgagcggac cggcaaggcg cgcggcaccg acctgctcga
     2161 cgggacgatc acgctgcctc tgatcgaggc cgcggcggcg gacccggcga tccgcgaagc
     2221 ggacctacac agcctcgaca ccgagagcgc cgaggcgctc tgtgaccgga tcgccgccac
     2281 cggtgcactc gaccgcgtgc gctcccgggc cctggagctg gtcgccgacg gcaagcgagc
     2341 gctcgagcgg gccgctttca cccccgagca acgccagctc ctcgccctcg tcgccgacgg
     2401 cgtcgtccag cgctacagct gaccgcttag ccctcctggc cggtggggcg gatgagcagc
     2461 tcgttgatcg cgacgtgggc cgggcgggtg acgatgtagg cgatcgcgtc ggcgatgtcc
     2521 ccggcctgca tgatctccat cgaggcgaag cgtttggcca gctgctcctg gatctcctcg
     2581 cggttgtggc tctgcagctc ggtgtcgacg gcgccgggct cgaccaggga gatccggacc
     2641 tggcgcccgg tgacctcctg gcgcagcgac tcgctgaagg cgccgactcc gtgcttggtc
     2701 gcgttgtaga cgcccgaccc tttgcgggcg atgcggccgg cgaccgagga gatgttgatc
     2761 aggtcggcaa ctccgcgagg ctcgccctcg gcgccggcga gcaggtgggg aagcgcggcg
     2821 cgagaggtgt agaggaggcc gagcaggttg acctcgatca tccgctccca ttcctcgacc
     2881 ggggccccgt tgatcggtcc gagcagcatc acgccggcgt tgttgacgag gacgtcgagc
     2941 cggccgagct ccgcgaccgt cttcgcaacc gccgcctcgg cctgctcacg ctcgctgacg
     3001 tcgacctcga tcggcagcgc ggtcccgccg gcgtcggcga tctccccgcc gagcgtctgc
     3061 aggcggtcgg cgcgacgggc ggcgatcgcc accgcggccc cctcggccgc cagcgctcgc
     3121 gcggtcgcct caccgattcc ggaggaggcg ccggtgacca gcgccacggt tccatcgagc
     3181 ttgcccatgc gccaacgcta cccaaccggg cacgccggca tgccagctag gtgaagtcgt
     3241 cggcgccgat caggtcggca tgcagagcgg cgacgaaggc gtcggccatg tcgctcatgt
     3301 tggcgtgctc gagcgcgcgc agctcgtcgg cgagcgtgtg ggcgccgatg tcgagctgct
     3361 cgtcgaaggc gtcgatctcg tcctcgccgt ggacgccctg gcagaaccac tcgcactccg
     3421 ggcagcgccg ccaaactcgc cagtgggcgc gatcgccagc ctgctcccag aaagtcggct
     3481 ggaccaggtc cgaaccgcac tcggggcaga tgtgcagatc cttgcgctgc ttggtcttgt
     3541 tgcgagccgg ctctctcatg tccctaacta atcgaacacc agaaacggaa acttgatccg
     3601 cctctttcga cacaagaccc acaaaacccc actaccgagt atctaaaacc tgctctggcc
     3661 ctgatccggc ggcgtccaca ccgcgttacc atggggcaga tgttcagcaa catcggccca
     3721 ctcgagatcg gcatcgtcct gatcatcgcc ttgatcgtct tcggcccgaa acgcctgccg
     3781 gagctcggca gcagcctcgg gcgcgggatc cgcgagttca aagagacggt caccggcgac
     3841 cgtcccgacg acgatgacga cgagcccgac gacaacgtca aagcgttgag cgcctcgacc
     3901 tcgaccgccg ccgaggccgc tgagcccgag gtcgcgtccg acaagcgcgg ctgaggcgcg
     3961 gaccccgatg gcgcgccgcg tcagggcgat ctcccacgag gaccgtctca gcctcgtcga
     4021 gcatctcgac gagctgcgcg gccgcctgat cgtctgcatc atcgcccttg ccaccgcctt
     4081 cgcgctgtgc atgtggcaga acaacctgct gctggacatc gcgcaggggc cgctgcccga
     4141 cgatcgcgat cagctgatca ccttcggggt caccgagccg ttcacgacca ccctgaccgt
     4201 cgtcacctac ggcgccctgg tgctgaccct gccggtgctg ctctggcagt tgtacgccta
     4261 cgtgttgccc gctttcagcc cgcgcgagaa gcgcgcggtg ctgccgatcc tgatcctctt
     4321 cccgatcctc ttcctcgccg gcctcgcctt cgcctactgg gtggtgatgc cggccgcggt
     4381 caagttcctg ctcagcttca acgacagcca gttcaacatc caggtgcggg cccgcgacta
     4441 ctacagcttc ttcgggatgg cgatgctggc cggcgggctc gtcttccaga tgccgctggt
     4501 gatcctcgcg attacccgcc tggggatcgt caaggtccag caactgacct ccaaccgccg
     4561 ctacgcgtat ctggtgatcg cggtcctggc cgcggcgctg ccgggagtcg atccgatctc
     4621 gatgctgatc gagatggtgc ccctgctgat tctgttcgag ttgagtatcg tcctcgcaaa
     4681 ggtattcgac cgaagacgag cagcgggggg agtggctgag ccctccacct agggagcgga
     4741 gcggcaaagc atggccaaaa agggcgggaa caaggacgag cacaagatgc tcttcgacct
     4801 gcgcgggaaa cggcgcaacg tggtcaaagt cgtctacgcg atcctcgccg tgctgatggg
     4861 cctgagcctg ctgctgatcg ccggcccgct gccgttcggt gacatcttcg gcgccgaaga
     4921 cgcacaggag gtcgcgcgcg agcaggccga agaacagcag cagcggatcg aagtgaaact
     4981 gaacaaagat ccggaagacc cgcagctcct gctcaccctc acccgcgccc agatccaggc
     5041 agggggccag ctggtcgaag aagtcgcccc cggacagctt gccctcacgg ttgagggacg
     5101 ccaggaatac gagaaagccg cttccacctg ggaggaatac ctggcggcca ccgacgaacc
     5161 gagtcccgcc ctggcgcagc agatggccaa cacctttctg acccttgcgc agacctcgcc
     5221 gagcggtccc gaagcagaga ccaacatcaa cgcatgggcg gaagcccaga agatcttcgc
     5281 gaaagagcgg cccagcctcg gctcgctgag cacgctggcg ctctacctgc cgttcacgtt
     5341 cgactacgca gcggcgaaac aagcggcgaa cgaagcgaag aaattcgcga cctcgaagtt
     5401 ccagcgcgaa cagatcgaca accagctcga agaagccgaa aaaaacgcca gggcattcca
     5461 gaaatcgctg aaagaagaag aacagctgaa ccagcagctg gaagaaagcc agagcggcgg
     5521 cggtgctgaa ggcggcggcg gagaaggtga cggcgaaggc ttcaccaacc ccttcaacgt
     5581 cggtggcgcc acttcctccg aatagctccc gagtgggccg cgaaggactc gaaccttcaa
     5641 ccttgagctt aagaggctcc tgctctaacc agttgagcta gcggcccggg atcggcaagt
     5701 ataggaagct cggcgccgcg aggtgggctc agctgccggg caggatgtgg cagtacttgg
     5761 agaccgcgcc gtcgatcacg gtcccgtctt cgaagctgaa gcgaccgcgg gtgcggagga
     5821 tgttgtcgct gcagcgggcc gaggtgtaac tgcgctgctt accgccgaag cggtagcggc
     5881 ggccgagctc gacttcgacc gcggtcaggg agccgagccc ggcggcgagc ggcgggattt
     5941 cgatcgtcgc gcgataggcg aattcgccgc ggcggcgttc gatcggcacg acgatcgcga
     6001 aggtctgggt ccccggctcg gtgaagtggg cgtggaggat cgcggtgggc cggccgccga
     6061 gcggcgtgcc gttgaagatc gtcagcggcg aggagacttc gaccaggccg gtggagagcg
     6121 agatcacgcc tttgaccgtg cccttgccga cctgcgccga cctgcaggcc tgccgcgctt
     6181 ccgcggtcgt cgcctgggcg atccgttcgg gcgggcaggt gggcaggcca cgcacgctga
     6241 ggcggccatc gtggtcgaag tcgacgacca gatggcgcag cggcaccggc cgggtgccgt
     6301 ctttgctggc gatgcgggcg tagccctcga actcgagcgg gacgaagcgg gagcggggca
     6361 gctggcgcgg ttcgaagctg ccgtcggcgc gcaggaccag gttgttcact tcgaccagcg
     6421 ccccccggcc actggccccg gccacaaccg cgagcgccgt caaggcgacg gcggcgatcg
     6481 tgaggcgttt cagccgacca cgcgacacgg cgaggtcaag agggctggaa agaggtcgcc
     6541 gttttcgaac tcgagcttgc ccttgacctg caggcgaccg ccggagcagg aggcgttgag
     6601 gtagccgacc ttgcgcccac cgcggttcca ggtccggccg atcgtcgctt cggcgagggt
     6661 cggcacgccg tagccgtcgg cgatctgcgg cagctcaacc tcgacgtggt agccataacg
     6721 gcccttgttc accttttcga cggcgatcgg caccagcaga gtcttctttg ccggcacggt
     6781 ctcgtaggcg tggccgatca ggctcggctg cccacccagc ttcggcccgt tgaagaagga
     6841 aaccgccgag ctgacctttt gctgtggttg gcccgggagg ttg
//
LOCUS       DASRIG010000039         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_541641, whole genome shotgun sequence.
ACCESSION   DASRIG010000039 DASRIG010000000
VERSION     DASRIG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541641"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFB52_01075"
     CDS             complement(<1..947)
                     /locus_tag="VFB52_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04954.1"
                     /translation="MHTQEIKELFSQAMVTLWSHKFRSFLTVLGVVIGTATTIGVASL
                     ASGMEAGFKEQIEQFGTNVAFVSRWGGGPRTSDLTEEERQRKPITIEDAIALSQLPSV
                     IAASPILRPSGLPPAVKFRGNEVRTPQVRGVWAGYSNTREVAIGRGRFFTEAEDEHRI
                     PVCVIGFDIAEKLLAGYEPVGKEIQIGNKVFVVIGVLEKAKSTFGGPPADSFIFIPYS
                     VMHAMYPAQIDHFVALGARQGQLDKLIDEATELLRRRRKVASDKPNNFEVTTPSGIIE
                     SFNKILLVVSVIVIPLVSVALLVGGIGVMNIMLVSVTERT"
     gene            complement(965..2188)
                     /locus_tag="VFB52_01080"
     CDS             complement(965..2188)
                     /locus_tag="VFB52_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04955.1"
                     /translation="MRFLEAVKLALASLRAHKLRSFLTLLGVIFGVMTVVAVAAVIEG
                     FFRYVDRAVTQDLGVNTVVLDKYGIITSFEDFMDANKRNKDIKIDDLAYLRGRMTLAQ
                     EIGATGESNTEIRAGDQKMTNISVRGVSAGMINIDTIQPELGRYVSEGDDERRRPVTM
                     VGVAVAEKLFGRRDVVGREIKINGLPFEVVGVAKEMGSTFGQSRDEFVTIPLSTYQKM
                     WGTRRSLNISIKAAEGARIEDVQDQARMLMRARHHLNYGDKDTFGFVTAEAINGFVQA
                     LFGIVAAVALGVTSISLVVGGIVIMNIMLVTVTERTREIGIRKSLGARRKDILMQFLV
                     ESTVLSAIGGLFGLVLAYGIARLLIAFTPIPAELPVWAAVLAIAVSSGVGMIFGLYPA
                     WKAAKLDPIVALRAE"
     gene            complement(2196..>2474)
                     /locus_tag="VFB52_01085"
     CDS             complement(2196..>2474)
                     /locus_tag="VFB52_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO04956.1"
                     /translation="EDRTAVGVCVVCFAISTSIGAVVAKLDDVFVKSERQGAGVGSAL
                     LSGLKEELRRQGVRRIDTSIHLRNEEARRFYERHGFLPLREERLACVL"
BASE COUNT          416 a          863 c          770 g          425 t
ORIGIN      
        1 gtccgctcgg tgaccgagac gagcatgatg ttcatgaccc cgatcccgcc gacgagcagc
       61 gcgacgctca ccagcgggat gacgatcacc gagacgacca ggagaatctt attgaagctc
      121 tcgatgatcc cgctcggcgt cgtgacctcg aagttgttcg gcttgtcgga ggccaccttg
      181 cgccgccgcc gcagcaactc ggtcgcctcg tcgatcaact tgtcgagctg cccctgccgc
      241 gcgccgagcg cgacgaagtg gtcgatctgc gccgggtaca tggcgtgcat gacgctgtag
      301 gggatgaaga tgaaactgtc ggccggcggt ccgccgaagg tgctcttggc cttctccagg
      361 acgccgatca cgacgaagac cttgttgccg atctgaatct ccttgcccac cggctcgtag
      421 cccgcgagca gcttctcggc gatgtcgaag ccgatgacgc agaccgggat gcggtgctcg
      481 tcctcggcct ccgtgaagaa gcggccgcgc ccgatggcga cctcgcgcgt gttggagtag
      541 cccgcccaga cgccgcgcac ctgcggcgtc cgcacctcgt tgccccggaa cttgacggcc
      601 ggcgggagcc ccgacgggcg caggatcggc gaggcggcga tgaccgacgg caactggctg
      661 agcgcgatcg cgtcctcgat cgtgatcggc ttgcgctgcc gctcctcctc ggtcaggtcg
      721 ctcgtccgcg gccccccacc ccaccggctg acgaaggcga cgttggtccc gaactgctcg
      781 atctgctcct tgaaccccgc ctccattccg ctcgccagcg acgccacccc gatcgtcgtc
      841 gccgtcccga tgaccacccc gaggaccgtc aggaacgacc ggaacttgtg cgaccagagc
      901 gtcaccatgg cctgggagaa gagctctttg atttcctgag tgtgcattgt gattagccga
      961 ccgctcattc cgcccttaag gccacgatcg ggtccaattt cgccgccttc cacgccgggt
     1021 agaggccgaa gatcatgccg acgccgctcg agacggcgat tgccaggacc gccgcccaga
     1081 ccggcagctc cgccgggatc ggcgtgaagg cgatgagcag tctcgcgatc ccgtaggcga
     1141 gcacgagccc gaagagcccg ccgatcgccg agagcaccgt cgactcgacg aggaactgca
     1201 tgaggatgtc tttgcggcgc gccccgagac tcttgcggat gccgatctcg cgcgtccgct
     1261 cggtgaccgt caccagcatg atgttcataa tcacgatccc gccgaccacc agcgagatcg
     1321 aggtcacccc gagcgcgacc gcggcgacga tcccgaagag cgcctggacg aagccgttga
     1381 tcgcctcggc cgtgacgaag ccgaaggtgt ccttgtcgcc gtagttcagg tggtgccgcg
     1441 cccgcatgag catccgcgcc tggtcctgga cgtcctcgat ccgcgccccc tcggccgcct
     1501 tgatcgagat gttcaggctc cgccgcgtgc cccacatctt ctggtaggtc gacagcggga
     1561 tggtcacgaa ctcgtcgcgc gactggccga aggtcgagcc catctccttg gccacgccga
     1621 cgacctcaaa cggcaggccg ttgatcttga tctctcggcc gaccacgtcg cgccgcccga
     1681 aaagcttctc ggcgaccgcc accccgacca tggtcaccgg gcgccgccgc tcgtcgtcgc
     1741 cctcgctgac gtagcgcccg agctccggct ggatcgtgtc gatgttgatc atgcccgccg
     1801 agaccccgcg gaccgagatg ttcgtcatct tctggtcgcc cgcccggatc tcggtgttgg
     1861 actctccggt cgccccgatc tcctgggcga gcgtcatccg cccccggagg taggcgaggt
     1921 cgtcgatctt gatgtccttg ttccgcttgt tggcgtccat gaagtcctcg aagctggtga
     1981 tgatcccgta cttgtcgagg acgacggtgt tgacgcccag gtcctgggtg acagcgcggt
     2041 cgacgtagcg gaagaagccc tcgatgacgg cggccaccgc gactaccgtc atgaccccga
     2101 agatcacgcc gagcagcgtg aggaacgagc gcagcttgtg cgcgcgcaag ctggcgaggg
     2161 cgagtttaac ggcttcaagg aatctcatag gcgactcaga gcacacaggc caggcgctct
     2221 tcgcggagcg ggaggaaccc gtgtcgctca taaaaacggc gcgcctcttc gtttctcaga
     2281 tgaatgcttg tgtcaatccg ccggacgccc tgccgccgca gctcctcttt gagcccgctg
     2341 agcagcgccg agccgacccc cgccccctgc cgctcggact tgacaaagac atcgtcgagc
     2401 ttggctacga cggctccgat cgaggtcgag atggcgaaac agaccacgca gacgccgacg
     2461 gccgtccgat cctc
//
LOCUS       DASRIG010000040         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1331630, whole genome shotgun sequence.
ACCESSION   DASRIG010000040 DASRIG010000000
VERSION     DASRIG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331630"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VFB52_01090"
     CDS             <1..1777
                     /locus_tag="VFB52_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZO04957.1"
                     /translation="SSDELAALSTALLLLTDGGFAYAEPLRLALQQVAWGHPNPLNEA
                     ERAPVEMAMTASAGGRDLSQRLSKIETAISRRKTIEFTYYTMERDETAKRKVDPFHLV
                     FRGGQFYLIGHAHERDAVRVFRLSRIQGKVGYASKAEHDFSPPEDFDRRDYGSRADWQ
                     LGEIQGTAKIFIRERIAWLIERDFGTYGTLRDAKKSDGAPGKGRIFETDYASHRELIA
                     WVLRWRANAQVLAPEELRGETESRVSLLRDRHENGFKPAKVVDRPLQDTPRRSRSNGR
                     GEAAIRPERFARLVTLAGMLIEAAKKGERLQIADLRQRLELTDEELREDVELLNVVNF
                     GGGTYVLYAEILGDEIEVDSEPYGDNFARPARLLPLEAKALVAAIDLFGDHLPQSDLQ
                     SAREKIVRALGHDPSEEGLEIVPTGGGDAAVARLVNDAIAGSKVLELSYYKENEDQLT
                     DRRVEPYRLENGKEGWYVECYDLGKEGVRHFKLDRIKEATLSDESFEPRPEVEEFAGV
                     EGWMTHGEVPTAEVARVWVSPDRARWLREERTVVEELADGAVVVELPYAGKPWLVREI
                     LRGAGDLVVLEPQDARDAIAKEVSG"
     gene            1812..>2345
                     /locus_tag="VFB52_01095"
     CDS             1812..>2345
                     /locus_tag="VFB52_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO04958.1"
                     /translation="MQQAPGHPEVLRILAPNPGPMTLEGTNTYVYGADPCVVIDPGSD
                     DAGHLAAIEAAAAERGGIGTVLLTHSHGDHTLGADQLGAEVVLPVGGEEHGGLRALAT
                     PGHAADHVAFLTADGVCFSGDLVLGLGSTIVPPGGNSLAAFLDSLRLLAAEPLELIAP
                     GHGPWVTDPAAKLAEYIE"
BASE COUNT          361 a          860 c          808 g          316 t
ORIGIN      
        1 ctccagcgac gagctggcgg cgctctcgac cgcgctgctg ctgctcaccg acggcggctt
       61 cgcctacgcc gagccgctgc ggttggccct gcagcaggtc gcctggggtc acccgaaccc
      121 gctcaacgag gccgagcgcg cgccggtcga gatggcgatg accgcctcgg ccggcggccg
      181 cgacctctcc cagcgcctct ccaagatcga gacggcgatc tcgcgtcgca agacgatcga
      241 gttcacctac tacacgatgg agcgcgacga gaccgcgaag cgcaaggtcg accccttcca
      301 cctcgtcttc cgcggtggcc agttctactt gatcggccac gcgcacgagc gcgatgcggt
      361 gcgcgtcttc cgcctctccc ggatccaggg caaggtcggt tatgcgtcca aggccgagca
      421 cgacttctcg ccgcccgagg acttcgaccg ccgcgactac ggcagccgcg ccgactggca
      481 gctcggcgag atccagggca ccgcgaagat cttcatccgc gagcggatcg cctggctgat
      541 cgagcgcgac ttcggcacct acgggaccct gcgtgacgcg aaaaagtccg acggggcgcc
      601 cggcaagggc aggatcttcg aaaccgacta cgcctcgcac cgcgagctga tcgcctgggt
      661 tctgcgctgg cgcgccaacg cccaggtgct cgcgcccgaa gagctgcgcg gggagaccga
      721 gagccgcgtc tcgctgctgc gcgatcggca cgagaacgga ttcaagccgg ccaaggtcgt
      781 cgaccggccg ctgcaggaca cgccgcggcg cagccgctcc aacgggcgcg gcgaggcggc
      841 gatccggccg gagcgcttcg cccgcctcgt caccctcgcc gggatgctga tcgaagcggc
      901 gaaaaagggc gagcgcctgc agatcgccga cctgcgtcag cgcctcgagc tgaccgacga
      961 ggagctgcgc gaggacgtcg agctgctcaa cgtcgtcaac ttcggcggcg gcacctacgt
     1021 gctctacgcg gaaatcctcg gcgacgagat cgaggtcgac tccgagccct acggcgacaa
     1081 cttcgcccgc ccggcgcgcc tgttgccgct ggaggcgaag gcgctggtcg ccgcgatcga
     1141 cctcttcggc gaccacctgc cgcagtccga cctgcagtcg gcgcgcgaga aaatcgtccg
     1201 cgcgcttggc cacgacccgt ccgaggaggg ccttgagatc gtccccaccg gcggtggcga
     1261 cgccgccgtc gcccgcctcg tcaacgacgc gatcgccggc tcgaaggtgc tggagctctc
     1321 ctattacaag gagaacgagg accagctcac cgaccgccgg gtcgagccct accggctcga
     1381 gaacggcaaa gagggctggt acgtcgagtg ctacgacctc ggcaaggagg gcgtccgcca
     1441 cttcaagctc gaccggatca aggaggcgac gctctcggac gagagcttcg agccgcgccc
     1501 cgaagtcgag gagttcgccg gggtcgaggg ctggatgacc cacggcgagg tgccgaccgc
     1561 cgaggtcgcc cgggtctggg tctcgccgga ccgggcgcgc tggctgcgcg aggagcgcac
     1621 cgtggtcgag gagctggccg acggcgccgt cgtcgtcgag cttccctacg cgggcaagcc
     1681 ctggctggtg cgcgagatcc tgcgcggcgc cggcgacctg gtcgtgctcg aaccccagga
     1741 cgcccgcgac gcgatcgcca aagaggtctc gggctgacgc ttgcggcgtt aggccggcta
     1801 taggcggctc gatgcagcaa gcgcccgggc atcctgaggt cctgcggatc ctcgccccga
     1861 atccggggcc gatgaccctc gaagggacca acacctacgt ctacggggcc gatccctgcg
     1921 tggtgatcga ccccgggtcc gacgatgccg gccacctggc ggcgatcgag gcggccgccg
     1981 ccgagcgcgg cgggatcggc accgtcctgc tcacccactc ccacggcgac cacacgctcg
     2041 gcgccgacca gctcggcgcc gaggtggtgc tgccggttgg cggcgaggag cacggcggcc
     2101 tgcgcgcgct cgccaccccc ggccacgccg ccgaccacgt cgccttcctg accgcggacg
     2161 gcgtctgctt cagcggtgac ctcgtgctcg ggctcggctc gacgatcgtg ccgcccggcg
     2221 gcaactcgct ggccgccttc ctcgactcac tgcgcctgct ggcggccgag ccgctggagc
     2281 tgatcgcccc cggccacggg ccctgggtca ccgatccggc ggcgaagctg gccgaataca
     2341 tcgag
//
LOCUS       DASRIG010000041         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_543916, whole genome shotgun sequence.
ACCESSION   DASRIG010000041 DASRIG010000000
VERSION     DASRIG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543916"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="gltX"
                     /locus_tag="VFB52_01100"
     CDS             <1..1437
                     /gene="gltX"
                     /locus_tag="VFB52_01100"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZO04959.1"
                     /translation="AAILPPRRRSGNRLHTLGHVNPPLRLRFAPSPTGALHIGGARTA
                     LYNWLAARHDGGELVLRIEDTDRERSTLENVDQILDALAWLELDWDGDPVSQVGRTEA
                     HEAALARLLESGAAYRDSATAKDVEAWKAEHGAGSGYRGTPTEEPGAAIRLRVPDEGE
                     MVVDDLLRGPISFPNRSYDDFVIARGDGTVLYNFAVAVDDAEMGITEVVRGDDHLSNT
                     PKQLLVLEALGYEPPRYAHLPLLHGPDGKKLSKRHGAASVQELREAGYLPAAVRNYLA
                     LLGWGTDDDTTLMSTEELVRRFRVADVGKAAAIFDEKKLRWLNGRFMRELPLEEYVEA
                     VAGQLERQGIAAPDPERLRAGCAIAQEKAQTLDEVWPLIRFLFEPPVEDPKAWKKVMK
                     EGSDELLEAAAQALAPVEPFEPEPIEVALAPLLERFEVKAGRLYQPIRVAITGTSISP
                     GIFESIATLGREPVLERVNAAVERLRAS"
     gene            1521..2651
                     /locus_tag="VFB52_01105"
     CDS             1521..2651
                     /locus_tag="VFB52_01105"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZO04960.1"
                     /translation="MSATTTVKKAAKSGNETEAPPEVSPKVAENGSSASRGGSAPAPG
                     SRLAEAFEAVERFPVLIESRERVIAAATADTARVGEVVEAVEADVALAISVLRFANRP
                     GMAGGGIASIPDAVEVLKPSGVLAIAGTAPSFDFFESNGGWELKPERFRVHALATQRA
                     ADRIGRVVGWAERDELAVAALLHDVGRLVISRLHPGYKVYFDAISRTPEQRLRDEREQ
                     LGIDHALVGGVLARRWNLPSRIAVAIERHHADDAEGLAALVASADMIAHYSQGEAISP
                     ERLRASAERCGLSTDALRDLLYELPMSRQESSRVSEPCPLSARELDVLRHLSEGMVYK
                     QIATEMHLSVSTIRTHLHNVYGKIGAVDRAQAVLTARDRGWI"
     gene            complement(2758..3354)
                     /locus_tag="VFB52_01110"
     CDS             complement(2758..3354)
                     /locus_tag="VFB52_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO04961.1"
                     /translation="MIDHHPLVRLGVRGVLEDRFEVHETESREEALDLIRDIGDFDVA
                     IIDMRPRQNGSGPALGAYDAIRDMHRSAPSLGIVAHGDRAHRHTASAALQAGASCYVA
                     RTAGPAALRKAVDAASAQERFVDPDVPPKGSRGVLTRRQRQILQLLANGESTTVAARE
                     LDLSEETVKTHTKNTLARLGARNRTHAVAIALRESLID"
     gene            3483..>3612
                     /locus_tag="VFB52_01115"
     CDS             3483..>3612
                     /locus_tag="VFB52_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZO04962.1"
                     /translation="MISITSKSRYAVVALAELARSGERPVPIKELAERRDIPEQFLE"
BASE COUNT          544 a         1285 c         1255 g          528 t
ORIGIN      
        1 gcggcgatcc tgccaccgcg gcgccggtcc ggcaaccggc ttcatacact cggccacgtg
       61 aacccgcccc tgcgccttcg ttttgcgccc tcgccaaccg gcgccctgca catcggcggc
      121 gcccgcaccg ctctctacaa ctggctcgcc gccagacacg acgggggaga gctggtgctg
      181 cggatcgagg acaccgaccg cgagcgctcg actctcgaga acgtcgatca gatcctcgat
      241 gcgctcgcgt ggctggagct ggactgggac ggcgatccgg tctcgcaggt ggggcggacc
      301 gaggcccacg aggccgccct ggcgcgcctg ctggagtccg gcgccgccta ccgcgactcg
      361 gccaccgcca aggacgtcga ggcgtggaag gccgagcacg gagccggcag cggttaccgc
      421 ggcacgccga ccgaggagcc gggagcggca atccggctgc gcgtccccga cgagggggag
      481 atggtggtcg acgacctcct gcgcggcccg atctccttcc cgaaccgcag ctacgacgac
      541 ttcgtgatcg cccgcggcga cggcaccgtg ctctacaact tcgcggttgc cgtagatgac
      601 gccgagatgg ggatcaccga ggtggtgcgc ggcgatgatc acctctcgaa cacccccaag
      661 cagctgctgg tcctggaggc gcttgggtat gagccgccgc gctacgcgca cctgccgctg
      721 ctgcacggcc ccgacggcaa gaagctctcc aagcgccacg gcgccgcctc cgtgcaggag
      781 ctgcgcgagg ccggctacct gccggcggcg gttcgcaact acctggcgct gctcggctgg
      841 ggcaccgacg acgacaccac cctgatgtcg accgaggagc tggtgcggcg cttccgggtc
      901 gccgacgtcg gcaaggcggc ggcgatcttc gacgaaaaga agctgcgctg gctgaacggc
      961 cgcttcatgc gggagctgcc gctcgaggag tacgtggagg cggttgccgg ccagctcgag
     1021 cggcaaggca tcgccgcgcc ggaccccgag cgcctgcgcg ccggctgcgc gatcgcccag
     1081 gagaaagccc agaccctgga cgaggtctgg ccgctgatcc gcttcctctt cgagcccccg
     1141 gtcgaggacc cgaaggcctg gaagaaggtg atgaaagagg gcagcgacga gctgttggag
     1201 gctgccgcgc aggccctggc gccggtcgag ccgtttgagc cggagccgat cgaggtggcg
     1261 ctggccccgc tgctcgagcg tttcgaggtc aaagccggcc gtctctacca gccgatcagg
     1321 gtcgcgatca ccggtacctc gatctcgcca ggcatcttcg agtcgattgc aacgcttggg
     1381 cgcgagcccg tgctggaacg cgtaaatgca gctgtagagc ggcttcgcgc gtcctgactg
     1441 gacggatgat cgaggtggga ggtcccgctc ggggtacgtt cggctaaacc aaacgtcttc
     1501 tcctgccgat gttgtgccac atgagcgcaa cgaccaccgt caaaaaggca gcaaaaagcg
     1561 gaaatgagac cgaggccccg cccgaggtct ccccgaaggt cgccgagaac ggcagcagcg
     1621 cctcccgcgg cggctccgca ccggcaccgg gcagccgcct ggccgaagcc ttcgaagcgg
     1681 tcgagcgctt ccccgtcctg atcgagtcgc gcgagcgagt gatcgccgcc gcgaccgccg
     1741 acaccgcccg tgtgggcgag gtcgtcgaag cggtcgaggc cgacgtcgcc ctggcgatct
     1801 cggtgcttcg cttcgccaac cgcccgggca tggccggcgg tggaatcgcc agcatccccg
     1861 acgcggtcga ggtgctgaag ccatcgggcg tgcttgcaat cgcgggtact gccccaagct
     1921 ttgatttctt cgagtccaac ggcggctggg agctgaagcc cgagcgcttc cgcgtccacg
     1981 cgctcgccac ccagcgggcc gccgaccgga tcggccgcgt ggtcggctgg gccgaacgcg
     2041 acgagctcgc ggtcgccgcc ctcctgcacg acgtaggccg cctcgtgatc tcccgcctac
     2101 acccgggcta caaggtctac ttcgacgcga tctcgcgcac ccccgagcag cgactgcgcg
     2161 acgaacgcga gcagctcggc atcgaccacg cactggtcgg cggcgtgctc gcccggcgct
     2221 ggaatctgcc ttcgcggatc gccgtcgcga tcgagcgcca ccacgccgac gacgccgagg
     2281 gcctcgccgc gctggtcgcc agcgccgaca tgatcgccca ctactcccag ggcgaggcga
     2341 tctcgccgga acggctgcga gccagcgccg agcgctgcgg gctcagcacc gacgcccttc
     2401 gcgacctgct ttacgagctg ccgatgtcgc gccaggaatc ctcgcgcgtg agcgagccct
     2461 gcccgctctc ggcccgcgag ctcgacgtcc tccgccacct ctccgagggc atggtctaca
     2521 agcagatcgc gaccgagatg cacctctccg tctccacgat ccgcacccac ctgcacaacg
     2581 tctacggcaa gatcggcgcg gtcgatcgcg cccaggccgt gcttaccgct cgcgaccgtg
     2641 gatggatcta aggggtcaca accccagccg ctgcgcagcg agtggcgccg ccgctcgctg
     2701 ctgaaggtcc gtcgccccgc ctggtcgggg cacgccgagc ctgacgcccg ccgcgactca
     2761 gtcgatcagc gactcgcgca gggcgatcgc gacggcgtgg gttcggttcc gcgcacctag
     2821 ccgggccagc gtgttcttgg tgtgggtctt gaccgtctct tcggagaggt cgagttcgcg
     2881 cgccgcgacc gtggtcgact cgccgttggc gagcagctgg aggatctggc gctggcgccg
     2941 ggtgagcacg ccgcggctgc ctttcggcgg cacgtcgggg tcgacgaagc gctcctgcgc
     3001 ggacgccgcg tcgaccgcct tgcgcagcgc cgccggaccc gcggtgcggg cgacgtaaca
     3061 gctggcaccg gcctgcaggg cggcgctggc tgtgtggcgg tgggcgcggt cgccgtgggc
     3121 gacgatcccc agcgacggcg ccgagcggtg catgtcgcgg atcgcgtcgt aggcgccgag
     3181 cgctgggccg ctgccgttct ggcgcgggcg catgtcgatg atcgcgacgt cgaagtcgcc
     3241 gatgtcgcgg atcaggtcga gcgcctcctc gcggctctcg gtctcgtgca cctcaaaacg
     3301 gtcctcgagg acgccgcgta cgccgaggcg aaccagcggg tggtgatcga tcaccaggca
     3361 gttgaggcgc gcgctcagga cgggatccgt tcgtaggtgg gcggttcggg cagcggttag
     3421 agccccgcac ccaatcgatt ccgatcctag cccagcggcg gtgggtgcgg tagaaagtcc
     3481 cggtgatctc gatcacctcg aagtcccgct acgcagttgt cgccctcgcc gagctcgccc
     3541 gttcgggcga gcggccggtg ccgatcaaag agctggccga gcgccgcgac attcccgagc
     3601 agttcctcga gc
//
LOCUS       DASRIG010000042         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3412674, whole genome shotgun sequence.
ACCESSION   DASRIG010000042 DASRIG010000000
VERSION     DASRIG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412674"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFB52_01120"
     CDS             <1..631
                     /locus_tag="VFB52_01120"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO04963.1"
                     /translation="AERPNSGPPATPRPAAGIVLLRRGGKHSQRDLEVLMLKRTEQAS
                     FMPGVWVFPGGSVDPEDGEGELGHRTCAVRELAEEASIELAPEEELVLFSRWVTPEAI
                     STRFDAWFFLALAPAHTPPEPDGVETTEAAWWKPADVLAAHEAGEMAIAFPTIHQLSW
                     LVPYATSDEALAAYRGRKVDSVMPKIIDDREGGGGWRPVLPGEPGYDEA"
     gene            complement(616..1317)
                     /locus_tag="VFB52_01125"
     CDS             complement(616..1317)
                     /locus_tag="VFB52_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04964.1"
                     /translation="MGAKQEAEEWVEIVRRYGERNGLTYEPVGGINPKDGPVALCVGG
                     VNRLTGQLAEGFWGSSCDADEKEIGSLFNKAVLPTALLAKAHMPDLAKVVPAFNVESI
                     EGHEQVTQHLQRKVEFESLDFNKRFLATVPKEYDPIQLRELFGPGFIAWVTTLSGEID
                     FGINDRQLWFLWRLGERSEAELKTALKNAGELFKRLQFEMDESGIHTYPPGPWHAGLE
                     PFPDAPAPPPAQASS"
     gene            1427..1990
                     /locus_tag="VFB52_01130"
     CDS             1427..1990
                     /locus_tag="VFB52_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZO04965.1"
                     /translation="MLIAIIVVVAIILIAVFYFIAKRNSFISSRNRVDESWSGIDVQL
                     KRRHDLVPNLVETVKGYAAHESETFEKTTKARAEAMAAQSVGETAQAEQHLTQALADL
                     KAVAENYPTLRATENFQQLSRNLSELEDEIQASRRIYNSNVQTYNTDIEQFPGSIVAN
                     AGNFEKREFFEIEDASEREPVAVSFDK"
     gene            2011..2205
                     /locus_tag="VFB52_01135"
     CDS             2011..2205
                     /locus_tag="VFB52_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZO04966.1"
                     /translation="MTSETKEATLWICDSCGFIYDPAIGDPDGGIPPGTAFEDIPKDW
                     FCPVCGARTSDFRPLEPGEA"
     gene            2224..3876
                     /locus_tag="VFB52_01140"
     CDS             2224..3876
                     /locus_tag="VFB52_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO04967.1"
                     /translation="MSQFDYDWIVVGSGFGGSVSALRLSEKGYSVGVLECGKRFEDKD
                     FPKSTWDLRRYFWAPRVGFKGIFRLSTFKDVSVVSGCGVGGGSLGYANTLYVPPSDFF
                     QDPQWGDMEDWEATLAPHYAEAQRMLGVVVHDNDDPADQLLREFGESIGVGDTYQKTP
                     VGIYFGEQPGETVPDPYFGGEGPDRTGCMQCGRCMVGCPHGAKNTLVKNYLYLAERRG
                     AVVTPERTVTEIRPLGAADGSDGYAITSERSGLLSGRGKRTLRARGVIVAAGPLGTNK
                     LLQRCRLGGALPKISNRLGELVRTNSESILAVTVPEDYPDDLTKRVAITSSIYPDPHT
                     HIETVTYGNDGDSMHLLYTLMVGDGTRVTRPLKLLAQIVRHPKKFAQTLWPKRWSRRT
                     IIVLVMQTLDNAIALRPKRNARTGAVRLQTEQDPERPMPTFIPVANEAAEWLAERTGG
                     VAQSATSEALMNIPTTAHILGGAVIGHGPEDGVVDSNQRVFGYENLLVCDGAAIPANV
                     GVNPSLTITALAEHAMSEVPPKGGTGDEPRQPEAARPATPIN"
     gene            complement(3873..4475)
                     /locus_tag="VFB52_01145"
     CDS             complement(3873..4475)
                     /locus_tag="VFB52_01145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZO04968.1"
                     /translation="MAFSVPDLPYPYDALEPHIDEATMRVHHDKHHQAYVDKANAALE
                     GTQWADADVEDVLKSLGDLPADKQGPVRNNAGGHYNHSLFWEMLSPDGGGEPSGELAE
                     AINSAFGSFDAFKDEFKNAGIGQFGSGWAWLVKNADGSVAVTKTPNQDSPISEGQTPL
                     LGADVWEHAYYLKYQNKRPEYLDAFWNVVNWDYVAERFSA"
     gene            complement(4518..5624)
                     /locus_tag="VFB52_01150"
     CDS             complement(4518..5624)
                     /locus_tag="VFB52_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04969.1"
                     /translation="MARRLVLLIAVLVAALPPAGAGADIPPGFIGLSPQNTGRASDFR
                     LMREAGVSSVRLPLFWHGVQSKSPYLADPDWKGFDREVALAAREGIRIMPFVLSTPEW
                     AAADPMTLPVHSPLQRWGWVSFLREAAERYGRDGEFWEESPGLPYLPVKRWEIWNEQN
                     IVSFDSNPNPARYALLLRMSGRTLHRTDPGSEVIIGGFFGQPLQIPPNVASGDYLNRI
                     YNAGNVKPYFDGVGLHPYIAGAGAMVGQMRTLRRVMRRRGDAATPIYVTELGWGSALG
                     PTRWEVGLQGQAEQLDRALSLISANRFRWRVRGVWWYSWTDEGGTCGFCRSSGLLTAD
                     REAKPSWYRFNRWTGGDPDTVPKLGEKEPEEEGG"
     gene            5717..5790
                     /locus_tag="VFB52_01155"
     tRNA            5717..5790
                     /locus_tag="VFB52_01155"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5751..5753,aa:Pro,seq:cgg)
     gene            5896..6069
                     /locus_tag="VFB52_01160"
     CDS             5896..6069
                     /locus_tag="VFB52_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04970.1"
                     /translation="MTDSEAVRIALLESAARRRARSSIAAEVERLAADEADRAEMRVI
                     RDQMAELAPDPVD"
     gene            6070..6387
                     /locus_tag="VFB52_01165"
     CDS             6070..6387
                     /locus_tag="VFB52_01165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZO04971.1"
                     /translation="MVRGEIFRLPAPRGVRGREQKGARYAVVVQADEFLALSTTLVAP
                     TSTSARPATFRPTISLDDTETMVLVEQTTVVDPERLGRSAGRLDSAELQAVDEALALV
                     LGL"
     gene            complement(6482..6901)
                     /locus_tag="VFB52_01170"
     CDS             complement(6482..6901)
                     /locus_tag="VFB52_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04972.1"
                     /translation="MADESVADRLRGFGVKGILVQMAERGQILELKCEMPQCYHPNGR
                     DKFESLATERRLWAPSRDHYPILRSAGGELRADNVRLSHVECNQRDHTRRKQIGALLL
                     AGESLKDIADMLNRKKTPAFHGTKRWTAAMVREAYVS"
     gene            7132..7404
                     /locus_tag="VFB52_01175"
     CDS             7132..7404
                     /locus_tag="VFB52_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZO04973.1"
                     /translation="MKVKLHRCPLMFVKIGAHPCWKVQKALDDQGIEYEVVKGPLGKG
                     KRDELEQLSGQRQYPVIELEDGSVYRDESSAMAARIEAGELGSPTG"
     gene            7441..8019
                     /locus_tag="VFB52_01180"
     CDS             7441..8019
                     /locus_tag="VFB52_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO04974.1"
                     /translation="MNRMMTPSPVAEEALPRELAEARFAQLYRAHEREILRYALRRSS
                     DPEDAADVVAETFLVAWRRLGDVPLGDEARLWLYATARRVLANHKRGIGRRTRLAERL
                     REELRRQLPVEPKAERRGVLAALAGMEEADRELLMLVGWEELAPSQAAQVLGISTMAA
                     RTRLHRARRRLRARLDEANERQANEMEVGEAR"
     gene            8016..>8456
                     /locus_tag="VFB52_01185"
     CDS             8016..>8456
                     /locus_tag="VFB52_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04975.1"
                     /translation="MNADEKYRELRAADPVPASKIDQLELGAAEAALAEAIVAEPAGG
                     EEPSVAATPWTSAGSRRRHRQLGLAGTVATAAAVAVALLLLLGGGASKTTSPAYGAEL
                     VQFAESTPLLLLEGPGWRVQNLSQNAPGEGQIEFVIGKPIPYETI"
BASE COUNT         1401 a         2922 c         2846 g         1287 t
ORIGIN      
        1 ggccgagcgg ccgaactccg gcccgccggc gacgccgcgg cccgctgccg ggatcgtcct
       61 gctgcggcgc ggcggcaaac attcgcagag ggatctcgag gtgctgatgc tgaagcggac
      121 cgagcaggcg agcttcatgc ccggcgtctg ggtcttcccc ggcggctcag tcgacccgga
      181 ggacggcgag ggcgagctgg gccaccgcac ctgcgccgtg cgcgagctcg ccgaggaggc
      241 ctcgatcgag ctggcgcccg aggaggagct ggtgctgttc tcgcgctggg tcaccccgga
      301 agcgatctcg acccgcttcg acgcctggtt cttcctcgcc ctcgcccccg cccacacacc
      361 gcccgagcct gacggcgtcg agaccaccga ggccgcctgg tggaaaccgg ccgacgtgct
      421 cgccgcacac gaggcgggcg agatggcgat cgccttcccg accatccacc agctcagctg
      481 gctggtcccc tacgcgacct ccgacgaggc gctcgccgcc taccgcggcc gcaaagtcga
      541 ctcggtaatg ccgaaaatca tcgacgaccg cgaaggcggg ggcggctggc ggccggtcct
      601 gcctggcgag cccggctacg acgaggcctg agcgggcggc ggcgccggcg cgtctgggaa
      661 cggctccaga ccggcgtgcc aggggccggg cgggtaggtg tggatcccgc tctcgtccat
      721 ctcgaactgc agccgtttaa acagttcgcc ggcattcttg agggcggttt tcagttccgc
      781 ctcgctgcgc tcgccgagcc gccagaggaa ccagagctgg cggtcgttga tcccgaagtc
      841 gatctcgccg ctcagcgtcg tcacccaggc gatgaagccg ggcccgaaca gctcgcgcag
      901 ctggatcggg tcgtactcct tcggcacggt cgccaggaag cgcttgttga agtcgagcga
      961 ctcgaactcg accttgcgct gtaggtgctg ggtgacctgc tcgtggccct cgatcgactc
     1021 gacgttgaag gcgggcacga ctttcgccag gtcgggcatg tgcgccttgg cgagcagcgc
     1081 ggtcggcagc accgccttgt tgaagaggct gccgatttcc ttttcgtcgg catcgcagct
     1141 cgagccccag aagccctcgg cgagctgccc ggtgagccgg ttgacgccgc cgacgcagag
     1201 cgccaccggg ccgtctttcg gattgatccc gccgaccggc tcgtaggtca ggccgttgcg
     1261 ctcgccgtag cggcggacga tctcgaccca ttcctcggct tcttgcttcg cgcccaccgc
     1321 gcgatcctac ggcagaggcg aagcgtccaa acgcctactc gacgccgacg atcggcggta
     1381 gcatgggcac cgagtcgatc cgaccccgga agccacagga gccccaatgc tcatcgccat
     1441 catcgtcgtc gtcgcgatca tcctgatcgc agtcttctac ttcattgcga agcgcaactc
     1501 gttcatctcc tcgcgcaacc gcgtcgacga gtcctggagc gggatcgacg tccagctcaa
     1561 acggcgtcac gacctcgtgc cgaacctggt cgagaccgtc aagggctacg ccgcgcacga
     1621 gagcgaaacg ttcgagaaaa cgaccaaagc gcgggccgag gcgatggccg cgcagagcgt
     1681 cggcgagacg gctcaggccg agcagcacct cacccaggcg ctggccgacc tcaaagcggt
     1741 cgccgagaac tacccgaccc tgcgcgccac cgagaacttc cagcagctca gccgcaacct
     1801 ctccgagctc gaggacgaga tccaggcctc ccgccggatc tacaactcga acgtccagac
     1861 ctacaacacg gacatcgaac agttcccggg ctcaatcgtc gccaacgccg gcaacttcga
     1921 gaaacgcgag ttcttcgaga tcgaggacgc cagcgagcgt gagccggtgg cagtcagctt
     1981 cgacaaatga ggctgcccta aactggctgc atgaccagcg agaccaaaga ggcaaccctc
     2041 tggatctgcg acagctgcgg gttcatctac gaccccgcga tcggtgaccc cgacggcggc
     2101 attcccccgg gaaccgcctt cgaggacatt cccaaggact ggttctgccc agtctgcggt
     2161 gcccgcacct ccgatttccg tcccctggag ccaggcgagg cctaagccac tagagtttgg
     2221 ctggtgagcc agtttgacta cgactggata gtcgtcggtt cggggttcgg gggatcggtc
     2281 tcggcgctgc gcctgtccga aaagggctac tcggtcggcg tgctcgagtg cggcaaacgc
     2341 ttcgaggaca aggacttccc gaagtcgacc tgggacctgc ggcgctactt ctgggcaccc
     2401 cgcgtcggct tcaaagggat cttccggctc agcaccttca aagacgtctc cgtggtcagc
     2461 ggctgcgggg tcggcggcgg cagcctcggg tacgcgaaca cgctttacgt gccgccctcg
     2521 gacttcttcc aggatccgca gtggggcgac atggaggact gggaggcgac gctggcgccc
     2581 cactacgccg aggcacagcg gatgctcggg gttgtcgtcc acgacaacga cgaccccgcc
     2641 gaccagctgc tgcgcgagtt cggcgagtcg atcggggtcg gcgacaccta ccagaagacc
     2701 ccggtcggga tctacttcgg cgagcagccc ggcgagaccg tgcccgaccc ctacttcggc
     2761 ggcgaggggc cggaccggac cggttgcatg cagtgcggtc gctgcatggt cggctgcccg
     2821 cacggggcga agaacaccct ggtcaagaac tacctctacc tggccgaacg gcgcggcgcc
     2881 gtcgtcaccc ccgaacgcac ggtaaccgag atccgcccgc tcggcgccgc cgacggcagc
     2941 gacggctacg cgatcaccag cgagcgctcc ggtctgctca gcggacgcgg caagcgcacg
     3001 ctgcgcgccc gcggcgtgat cgtcgccgcc ggcccgctcg gcaccaacaa gctgctgcag
     3061 cgctgccgcc tcggcggcgc cctgccgaag atctccaacc ggctcggcga gctggtccgc
     3121 acgaacagcg agagcatcct cgcggtcacc gtgcccgagg actaccccga cgacctgacc
     3181 aaacgggtcg cgatcacctc cagcatctac cccgaccccc acacccacat cgagaccgtc
     3241 acctacggca atgacggcga ctcgatgcac ctgctctaca cgctgatggt cggcgacggc
     3301 acccgggtga cccggccgct gaagctgctc gcccagatcg tccgccaccc gaagaagttc
     3361 gcgcagacgc tgtggccgaa acggtggtcg cggcgcacga tcatcgtcct ggtgatgcag
     3421 acgctcgaca acgcgatcgc cctacggccg aagcgcaacg cgcgcaccgg cgcggtgcgg
     3481 ctgcagaccg aacaggatcc cgagcggccg atgccgacct tcatcccggt cgccaacgaa
     3541 gccgccgagt ggctggccga gcggaccggg ggcgtcgccc agagcgccac ctcggaggcg
     3601 ctgatgaaca tcccgaccac ggcccacatc cttggcggcg cggtgatcgg ccacggcccc
     3661 gaggacggcg tcgtcgactc caaccaacgc gtcttcggct acgagaacct gctggtctgc
     3721 gacggcgccg cgatccccgc gaacgtcggc gtcaaccctt cgctgacgat cacggctctg
     3781 gccgagcacg cgatgagcga ggtcccgccg aaaggcggaa ccggggacga gccgcgccag
     3841 cccgaggcgg cgcggcccgc gaccccgatc aactaagcgc tgaagcgctc agcgacgtag
     3901 tcccagttga cgacgttcca gaacgcgtcg aggtattcgg gacgtttgtt ctggtacttc
     3961 aggtagtacg cgtgctccca gacgtcggcg ccgagcagcg gcgtctgacc ctcggagatc
     4021 ggcgagtcct ggttcggggt tttggtcacg gcgacgctgc cgtcggcgtt cttgaccagc
     4081 cacgcccagc cggagccgaa ctggccgatg ccggcgtttt tgaactcgtc cttgaaggcg
     4141 tcgaaggagc cgaaggcgct gttgatcgcc tcggccagct cgccgctcgg ctcgccgccg
     4201 ccgtccgggc tcagcatctc ccagaagagg ctgtggttgt agtggccgcc ggcgttgttg
     4261 cggaccgggc cctgcttgtc ggcgggcagg tcaccgaggc tcttgagcac gtcctcgacg
     4321 tcggcgtcgg cccactgcgt gccctcgagc gcggcgttgg ctttgtcgac gtaggcctgg
     4381 tggtgtttgt cgtggtggac gcgcatcgtc gcctcgtcga tgtgcggctc gagcgcgtcg
     4441 taggggtacg gaagatcggg taccgagaat gccatcgaaa ggtctccttg ctggtttgaa
     4501 tgcctccacc aaggagccta tcccccctcc tcttccggct ccttctcgcc gagcttgggc
     4561 acggtgtccg gatccccccc ggtccagcgg ttgaagcggt accaggaggg cttcgcttcg
     4621 cggtcggcgg tcagcaggcc ggaggagcgg cagaagccgc aggtcccgcc ctcgtcggtc
     4681 cagctgtacc accagacgcc gcgcacgcgc cagcggaagc ggttggcgga gatcagcgag
     4741 agggcgcggt cgagctgttc ggcctggccc tggagaccga cctcccagcg ggtcgggccg
     4801 agggccgagc cccagcccag ctcggtcacg tagatcggcg tggcggcatc gccgcggcga
     4861 cgcatcaccc gccgcaacgt gcgcatctgg ccgaccatcg cgccggcgcc ggcgatatac
     4921 gggtgcaggc cgaccccgtc gaagtacggc ttcacgttgc cggcgttgta gatccggttc
     4981 aggtagtcgc cgctggccac gttgggcggg atctgcagcg gctggccgaa gaagccgccg
     5041 ataatcactt ccgagccggg gtcggtgcgg tgcagcgtgc gtccggacat ccgcaggagc
     5101 aacgcgtacc gggctgggtt ggggttgctg tcgaagctga cgatgttctg ctcgttccag
     5161 atctcccagc gcttgaccgg caggtagggc agcccggggc tctcctccca gaactccccg
     5221 tcccgcccgt agcgctcggc cgcctcgcgc aggaagctga cccagcccca gcgctgcagc
     5281 gggctatgaa ccggcagcgt catcggatcg gcagcggccc actcgggcgt gctcagaacg
     5341 aacggcatga tccggattcc ctcgcgcgcg gcgagcgcga cttcccggtc gaaccccttc
     5401 caatccggat cggcgagata ggggctcttc gactgcaccc cgtgccagaa aagcggcaac
     5461 cgcacgctcg agaccccggc ttcccgcatc agcctgaagt ccgaagcccg ccccgtgttc
     5521 tgcggcgaca acccgatgaa tcccggcggg atgtccgcgc ccgcgcccgc cggcggcaga
     5581 gccgccacga gcaccgcgat caggaggact agcctgcggg ccatagccat cgaagaacca
     5641 ttcgcgaggc aaagtagcgg ccgcgaccat agggagaccc cgcccccacc ccccgctctg
     5701 ggataatcgc cgctctcggg gcgtggcgca gcctggtagc gcgcaccgtt cgggtcggtg
     5761 aggtccccgg ttcaaatccg ggcgccccga tttgtgtagc acgatgcgtg ctacattgac
     5821 ctcatgagtc gctcgcttca cgttcgcctg gacgacagtt ccgcagcagc gcttcggatc
     5881 gttcgcgggg gctcgatgac tgattccgag gccgtgcgaa tcgccctctt ggagtccgcc
     5941 gcccgacgaa gggcgcgctc gtcgatcgcg gcggaggtgg agcggctggc cgccgacgag
     6001 gctgaccggg ccgagatgcg ggtcatccgc gatcagatgg ccgaactggc accggatccg
     6061 gtcgactagg tggttcgcgg cgagatcttt cggctgccgg ctcctcgcgg cgttcggggc
     6121 agggagcaga aaggcgcccg ctacgccgtg gtggtccagg cagatgagtt cctggcgctg
     6181 agcacgacgc tggtcgcgcc gacctcgacc agtgctcgcc cggcgacctt ccgcccgacg
     6241 atttcgctcg acgacaccga gacgatggtc ctggtcgagc agaccacggt cgtggacccc
     6301 gagcgcttgg ggagatccgc aggacgcctc gactccgcgg agctgcaagc cgtcgacgaa
     6361 gcgctcgcgc tggtcctcgg cctctaagcc cggccgcccg aacggcagaa aaaggacctg
     6421 aacgtgtcgc tgatgtttcc caagatccgg cagttgcctt tcgcgcgaag cggaacccgg
     6481 actaggacac gtaggcctcg cgaaccatgg ccgccgtcca tcgtttcgtt ccgtgaaacg
     6541 ccggggtctt cttgcggttc agcatgtcgg cgatgtcttt cagcgattcg cctgcaagaa
     6601 gcagtgcccc gatctgcttc cgccgcgtgt ggtcacgctg attgcactcg acatgggaca
     6661 gccgaacgtt gtcggcacgc agctctccac cggctgacct gaggatcggg tagtggtcgc
     6721 gcgagggcgc ccacaacctg cgctccgtcg cgagcgattc gaacttgtcg cgtccgttgg
     6781 ggtgatagca ctgcggcatc tcgcacttca gctcaagaat ctgcccacgt tcggccattt
     6841 gcacgaggat gcccttcacg ccgaatccgc gaagtcggtc cgcgaccgat tcatctgcca
     6901 tgcggcgatc ctgacacaac tgctctcgcg tgaaagcagc agcgccccga ttttcggcaa
     6961 cgaacgagcc gcccgcgcca tgcgacggag ggactctgtg atacaggagt gatgcacacc
     7021 actcctcaga cccgcttgta gcgccgacct gatccgggcg ccccgactta aagacctcat
     7081 tcgacgcgac caattcaccg caacggtccc tgaccggact aggattgcgg aatgaaagtc
     7141 aagctccatc gctgcccgct catgttcgtg aagatcggtg cgcatccgtg ctggaaggtg
     7201 cagaaggcgc ttgatgacca ggggatcgag tacgaggtcg tcaagggacc gctggggaaa
     7261 gggaagcggg acgagctgga gcagctctcg ggacagcgcc agtaccccgt gatcgagctc
     7321 gaggacggaa gcgtctatcg cgacgagtcg agtgcgatgg ccgcgcgcat cgaagcgggc
     7381 gagctcggct cacccaccgg ttaggcggcg ccccggtgta acgctcggcc gagtccggac
     7441 atgaaccgga tgatgacccc gagtcccgtc gccgaagagg cgctgccccg tgagctcgcg
     7501 gaggcgcggt ttgcgcagct ctaccgagcg cacgagcgcg agatcctgcg ctacgcgctg
     7561 cggcgcagct ccgaccccga ggacgcggcc gatgtggtcg cggagacctt cctggtcgcc
     7621 tggcgccggc tcggagacgt gccgctcggg gacgaggcgc ggctctggct ctacgcgacc
     7681 gcgcggcggg tgctcgccaa ccacaagcgc gggatcggcc ggcgcacgcg gctggcggag
     7741 cggctgcgcg aggagctgcg ccgacagctc cctgtggagc cgaaggccga gcgtcgtggc
     7801 gtgctggcgg cgctcgccgg gatggaggag gccgatcgcg agctgctgat gctggtcggc
     7861 tgggaggagc tggccccgag ccaggcggca caggtgctcg ggatctcgac gatggcggcc
     7921 cgcacccggc tgcacagggc acgccgccgc ctgcgcgcgc gactcgacga ggcgaacgag
     7981 aggcaggcga acgagatgga ggtaggtgag gcacgatgaa cgcggacgag aaatatcgag
     8041 agctgcgagc ggccgatccg gtgcccgcgt cgaagatcga ccagctcgag ctcggggcgg
     8101 cggaggcggc gctcgccgag gcgatcgtcg ccgaacccgc cggcggcgag gagccgagcg
     8161 tcgcggcgac gccctggacg agcgccggca gccgccgtcg ccaccgccag ctcggcctcg
     8221 ccgggacggt cgccaccgcg gccgcggtgg cggtggcgct gctgctcctg ctcggcggcg
     8281 gcgcctcgaa gaccacgtcc ccggcctacg gcgccgagct ggtccagttc gccgagtcga
     8341 cgccgctgct gctgctcgaa ggccccggct ggcgggtcca gaacctgtcg cagaacgcac
     8401 ccggcgaagg gcagatcgaa tttgtcatcg gcaagccgat cccctacgaa acgatc
//
LOCUS       DASRIG010000043         6445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2893108, whole genome shotgun sequence.
ACCESSION   DASRIG010000043 DASRIG010000000
VERSION     DASRIG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893108"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFB52_01190"
     CDS             complement(<1..699)
                     /locus_tag="VFB52_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04976.1"
                     /translation="MGKSRRLIFILAATAVLAVVASGCVGYKPDSFNVSQVGGIGPVK
                     MHLELCSGEAKTTAPSCAGNDESNQAQSMLGFIVPKGSIAPATVTAVPGPGATPIKYN
                     RNDELAAAADGEELPPGLELVGYLSEVYSEQAGDELEWSVDAEFGLPPAADGGSFAGN
                     FQTSSLIGWRVVDGTHSASRPINCTELEGPESFASGCSSTGELIEYGVSNLVIKPPAT
                     VSVYAGAKATVPFSL"
     gene            complement(713..2575)
                     /locus_tag="VFB52_01195"
     CDS             complement(713..2575)
                     /locus_tag="VFB52_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04977.1"
                     /translation="MLALVRRWGAPSASLAVVLSLALAPGAAAETTRFSGVVEGGLSV
                     TGNSLGLSKKAGANEAGTEDSIGALIGGTGTVGDFPAGTTLDWTQDGSSAWLVLPEDA
                     EVVYAELVWGGSYEVGVESLAPSLGSFVNLSTGGSGFAAAPVDETSETVSITADGGFQ
                     VREYMRSSDVTGFVAEHGAGLYRAAGIPITRAAASNTLNSGGWALYVVYEAASEPVRW
                     VEFATGGGFIQETDSYDYGFDDYCAPQVETPSARLRVTASEGDANRVGDRLLAGSGPL
                     ALAALSSPNNPEDNFFASQVNLANGELDTEGTFGERNHNAATGTNMLAGRQGWDVGAA
                     QVSGIANGQEGLLARAATVGDTYMVNGLGLEATIDAPEFAAGQGSLGVSSLAVGESTT
                     LTVPLENTGALSAGDVFFALPLPAGVEPTSFAINGAPVPVTRESLADGIEVGDVDPES
                     SRTITVGLKATAAGSFELAPEWAYRFAQCAGPDPAEGVAGGAVLTLTATQPPGPPSGP
                     AGGGGGGSTAVAGKLSFGKLTLNKKKGTATLAVSVNGAGTLSTSGKGLVAFKRSTSGP
                     QTVKVAIKAKGSAKTVLAKTGKVKIDVSFKFQPQAGAAVTKKKAVVLKNSGVRG"
     gene            complement(2585..3568)
                     /locus_tag="VFB52_01200"
     CDS             complement(2585..3568)
                     /locus_tag="VFB52_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZO04978.1"
                     /translation="MRAMVFERVGEPLRAAELPDPEPGPGQVLIAVRACGVCRTDLHI
                     VDGELSEPKLPLVPGHQIVGEVVGAGEGAERFASGDRVGVPWLGWTDGTCRYCAGGRE
                     NLCDAARFTGYDLDGGYAELTVADERYCLALPDGYGDAEAAPLLCAGLIGYRALRLVG
                     EAERIGFYGFGSSAHILCQVAVHEGRRVFAFTREGDVEGQEFARELGAEWAGSSEELP
                     PEPLDGAIVFAAVGALMPAALRASAKGARVISAGIHMSDIPSFPYEILWEERTLGSVA
                     NLTRADGEQFLALAPRVPVRAEITTYPLERANEAIADLRAGKFRGSAVLTP"
     gene            3649..4998
                     /locus_tag="VFB52_01205"
     CDS             3649..4998
                     /locus_tag="VFB52_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZO04979.1"
                     /translation="MDRPAQQPGPAPQDGSDEEARLNRVVSKPMLVFFILGDILGGGI
                     YALVGEVGGEVGGAIWLAFIVALLLAALTAASYAELVTKYPRAGGASLYAKRAFNSQL
                     VAFLVAFAVMCSGVTSASALARAFGGDYLAEFISIDVVIAALAFIAIVAIVNYVGILF
                     SVRANLVMTMVELTGLLLVVIIGLAALGDGVGEPSRAFEFKEGTGPLLAILAGAAVAF
                     YALIGFEDSVNIAEETREPRLVYPFALFGGLAAAGLIYLAVTMIASMAVPTDKLIGSS
                     GPLLEVVQVGPLAISTKVFSAIGLLALANGALINMIMASRLLYGMSNEQVLPSPFGRL
                     SPRRTPWVAIAFTTLLSAGLILSGDLGSLADTTVLLLLCVFIVVNISVLVLRRDPVEE
                     KHFEVPRAIPVLGVAGCVLAMTQVEGETWLRAAALLAIGLVLWAVERLATARVATSP"
     gene            5008..5955
                     /locus_tag="VFB52_01210"
     CDS             5008..5955
                     /locus_tag="VFB52_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZO04980.1"
                     /translation="MLFFTHPASLEHDPGELMPGHPDTPERIVAIEAALHERGWLGME
                     RREAPAASERQLELVHTPRLIAEVREMSEAGGGRIDADTAVNAATYRAAVHAAGGACE
                     LTRALLRGEAEVGYSCMRPSGHHAEPDRAMGFCFFDNVAIAAAMAIADHGLERVMVLD
                     WDVHHGNGTGEIFRRRDDVLFFSLHQSPLYPGTGPASDKGSGPGEGFTVNLPVAPGSG
                     EDVWLGLIDEVVIPTTERYRPQLILVSAGYDAHREDPLASCALESTSFAAMATRVGDL
                     AARLGVPLGLVQEGGYHVPSLVESLLATLGALGAQPAEA"
     gene            complement(5957..>6445)
                     /locus_tag="VFB52_01215"
     CDS             complement(5957..>6445)
                     /locus_tag="VFB52_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04981.1"
                     /translation="SISMPQRTYAPGAVAGATFRAEAATRSVVVNVPNGAKPGVYEVT
                     ITATAAGGGAATALAKLKVQRPTIKVLGKPKLNRRAGTAVLKVQVPGAGTVTLSGKGV
                     AVAKKSAKGPKTLKLPLAAKGAAKLALLANGSVKLRPKLKFKPDSGAAVKKTTAITLR
                     LG"
BASE COUNT          849 a         2378 c         2202 g         1016 t
ORIGIN      
        1 gagcgagaac ggcacggtgg ccttggctcc ggcgtagaca ctgaccgtcg ccggcggctt
       61 gatcaccaga ttggaaacgc cgtattcgat caattcgccg gtcgagctgc agccgctggc
      121 gaacgattcc gggccttcga gttcggtgca gttgatcggg cgactggccg agtgggtgcc
      181 gtcgacgacc cgccagccga tcaggctgga ggtctggaag ttgccggcga acgagccgcc
      241 atccgccgcg ggcggcaggc cgaattccgc atcgacgctc cattcgagtt cgtcacccgc
      301 ctgttcgctg tacacctcgg acaggtagcc gacgagttcc agcccgggcg gcagttcttc
      361 gccgtctgcg gcggctgcga gttcgtcgtt gcggttgtat ttgatcggcg tcgcgccggg
      421 cccgggcacc gcggtcaccg tagccggggc gatcgagcct ttgggcacga tgaagccgag
      481 catcgactga gcctggttgc tctcgtcgtt gcccgcacag ctaggcgcgg tcgttttcgc
      541 ttcgccgctg cacagctcga ggtgcatctt caccggaccg atgccgccca cctggctgac
      601 gttgaacgaa tccggcttgt agccgacgca gccgctggcg acgacggcca gcacagccgt
      661 cgcggcaaga atgaaaatca ggcgccgaga cttgcccatc gagcgcggca gtctagccgc
      721 ggacgccgct gttcttcaac acgaccgctt tcttcttggt caccgcggcg ccggcctgcg
      781 gctggaactt gaagctgacg tcgatcttca ccttgccggt cttcgccagc accgtcttgg
      841 cgctgccctt ggccttaatc gcaaccttca ccgtctgtgg cccggaggtg gaccgcttga
      901 aggcgaccag gcccttgccc gaggtgctca gcgtgccggc gccgttgacg ctcaccgcca
      961 gcgtcgccgt ccccttcttc ttgttcaggg tcagcttgcc gaagctgagc ttgcccgcga
     1021 cggcggtgga gccaccccct cccccaccgg cgggaccgct cggcgggccg gggggctgcg
     1081 tcgcggtcag ggtcagcacc gcgcccccgg ccacgccctc agccgggtcg ggtcccgcgc
     1141 actgcgcgaa acggtaggcc cactccggcg ccagctcgaa gctgccggcg gcggtcgctt
     1201 tgaggccgac cgtgatcgtg cggctggact cggggtcgac gtcgccgacc tcgatcccgt
     1261 ccgcgagcga ttcccgggtc accggcaccg gcgcgccgtt gatcgcgaac gaggtcggtt
     1321 cgaccccggc cggcagcggc agcgcgaaga agacgtcgcc ggccgagagg gcgccggtgt
     1381 tttccagcgg cacggtcagc gtcgtgctct ccccgaccgc gagcgagctg acgccgagcg
     1441 agccctggcc cgcggcgaac tccggggcgt cgatcgtcgc ttccaggccg agcccgttga
     1501 ccatgtaggt gtcgccgacc gtcgccgctc gggcgagcag gccttcctgg ccgttggcga
     1561 tgccggatac ctgagcggcg ccgacgtccc agccctggcg tccggcgagc atgttggtgc
     1621 ccgtggcggc gttgtggttg cgttcgccga aggtgccctc ggtgtcgagt tcgccgttcg
     1681 cgaggttgac ctgcgaggcg aagaagttgt cttccgggtt attggggctg gagagcgcag
     1741 ccagcgccag cgggccggag ccggcgagca gccggtcgcc gacccggttg gcgtcgccct
     1801 cgctggcggt cacccgcagg cgagcgctcg gcgtctcaac ctggggcgcg cagtagtcgt
     1861 cgaagccgta gtcgtaggag tcggtttcct ggatgaaccc cccgccggtg gcgaactcga
     1921 cccagcggac cggctccgat gccgcctcgt agacgacgta gagcgcccag ccgccggagt
     1981 tcagggtgtt gctcgccgcc gccctggtga tcgggatgcc ggccgcccgg tagaggccgg
     2041 cgccgtgctc ggccacgaac ccggtcacgt cggacgagcg catgtattcc ctcacctgga
     2101 agcctccatc agccgtgatc gagaccgttt ccgaggtttc gtccacgggc gcggccgcga
     2161 agccgctgcc cccggtgctc aggttcacga acgagccgag agagggcgcc aagctttcga
     2221 cgccgacttc gtaggagccg ccccagacca gctccgcgta aaccacctcg gcgtcttccg
     2281 gcagcaccag ccaggccgag gatccgtcct gcgtccagtc gagcgtcgtc ccggcgggga
     2341 agtccccgac cgtcccggtc ccgccgatca aagccccgat cgaatcctcg gtgccggctt
     2401 cgttcgcgcc ggctttctta ctcagcccga gcgagttgcc ggtcaccgag agcccgccct
     2461 cgacgacccc gctgaagcgc gtcgtctcgg ccgcggcgcc gggggcgaga gccagcgaga
     2521 ggacgactgc gagcgatgcg gacggcgctc cccagcgccg gacaagagcg agcacgcgcg
     2581 gattctacgg cgtcagaacg gcggatccgc ggaacttccc ggcgcgcaga tccgcgatcg
     2641 cctcgttggc ccgctcgagc ggataggtcg tgatctcggc gcgcaccggg acccgcggcg
     2701 ccagcgccag gaactgctcg ccgtctgccc gggtcaggtt ggcgaccgag ccgagcgtgc
     2761 gctcctccca gagaatctcg taggggaacg aggggatgtc gctcatgtgg atcccggcgc
     2821 tgatcacccg cgcgcccttg gcgctcgccc gcagcgccgc cggcatcagc gccccgaccg
     2881 cggcgaagac gatcgcgccg tcgagtggct ccggcggcag ctcctccgag gagcccgccc
     2941 actcggcgcc gagttcgcgc gcgaactcct ggccctccac gtcgccctcg cgggtgaacg
     3001 cgaacacgcg ccgcccttcg tggaccgcga cctggcagag gatgtgggcg gaggagccga
     3061 agccgtagaa gccgatccgc tccgcctcgc cgaccaggcg cagcgcccgg tagccgatca
     3121 gccccgcgca gagcagcggc gccgcctccg cgtcgccgta gccgtcggga agcgccaggc
     3181 agtagcgctc gtcggcgacg gtcagctccg cgtagccacc gtcgaggtcg tagccggtga
     3241 agcgggccgc gtcgcagagg ttctcgcggc cgcccgcgca gtagcggcag gtcccgtcgg
     3301 tccagccgag ccagggcacc ccgacccggt cgccggacgc aaaccgctcc gccccctccc
     3361 ccgccccgac cacctcgccg acgatctggt ggcccggcac cagcggcagc ttcggctcac
     3421 tgagctcgcc gtcgacgatg tgcaggtcgg tgcggcagac cccgcaggcg cggaccgcga
     3481 tcagcacctg cccggggccg ggctcggggt cgggcagctc ggccgcccgc agcggctcac
     3541 cgacccgctc gaagaccatc gcgcgcatcg ctccatgatg gcgcctgctt gccatcctgc
     3601 ccacccgggt agctgcggcc caccttcaat cggcaggagg cggcagaggt ggatcgtccc
     3661 gcgcagcaac ctggtcccgc accgcaagac ggcagcgacg aggaggcccg cctcaaccgg
     3721 gtcgtctcca aaccgatgct cgtcttcttc atcctcggcg acatcctcgg cggcggcatc
     3781 tacgcgctgg tcggcgaagt cggcggcgag gtcggcgggg cgatctggct cgccttcatc
     3841 gtcgcgctgc tgctcgccgc gctcaccgcc gcctcctacg cggagctggt caccaagtac
     3901 ccgcgcgccg gcggcgcctc gctgtatgcg aagagggcgt tcaacagcca gcttgtcgcc
     3961 ttcctcgtcg ccttcgcggt gatgtgctcc ggggtcacct ccgccagcgc cctggcgcgc
     4021 gccttcggcg gcgactacct ggccgagttc atctcgatcg acgtggtgat cgcggcgctt
     4081 gccttcatcg cgatcgtcgc gatcgtcaac tacgtcggca tcctcttctc ggtccgcgcc
     4141 aacctggtga tgacgatggt cgagctgacc ggcctgctgc tcgtcgtgat catcggcctg
     4201 gcggcactcg gcgacggcgt cggcgagccc tcgcgcgcct tcgagttcaa ggaggggacc
     4261 gggccgctgc tggcgatcct ggccggcgcc gcggtcgcct tctacgcgct gatcgggttc
     4321 gaggactcgg tcaacatcgc cgaggagacg cgcgagccgc gcctggtcta cccgttcgcg
     4381 ctgttcggcg gcctcgccgc ggccgggctg atctacctcg cggtgacgat gatcgcctcg
     4441 atggcggtgc cgaccgacaa gctgatcggc tcctccggcc cgctgctcga ggtggtccag
     4501 gtcgggccgc tggcgatctc gaccaaggtg ttctcggcga tcggcctgct ggcgctcgcc
     4561 aacggcgccc tgatcaacat gatcatggcc tcgcgcctgc tctacgggat gtcaaacgag
     4621 caggtcctgc cctcgccctt cggccggctc agcccgcgcc gcaccccctg ggtcgcgatc
     4681 gccttcacca ccctgctctc cgccgggctg atcctctccg gcgacctcgg ctcgctcgcc
     4741 gacaccaccg tgctgctgct gctctgcgtc ttcatcgtgg tcaacatctc ggtcctggtc
     4801 ctgcgccgcg atccggtcga ggagaagcac ttcgaagtgc cacgcgcgat cccggtgctc
     4861 ggcgtcgccg gctgcgtgct ggcgatgacc caggtcgaag gcgagacctg gctgcgtgcc
     4921 gccgcgctgc tggcaatcgg cctcgtcctc tgggccgtcg agcgcctcgc caccgcccgc
     4981 gtcgccacct cgccgtagag tcggcgggtg ctcttcttca cccaccccgc ctcgctcgaa
     5041 cacgacccgg gtgagttgat gcccgggcac ccggatacgc cggaacggat cgtcgcgatc
     5101 gaggcggcgc tgcacgagcg gggctggctc gggatggagc ggcgcgaggc gccggcggcg
     5161 agcgagcgcc agctggagct ggtccacacg ccgcgcctga tcgccgaagt gcgcgagatg
     5221 tcggaggccg ggggcgggcg gatcgacgcc gacaccgcgg tcaacgccgc cacctaccgg
     5281 gcggcggtgc acgcggccgg gggtgcctgc gagctgaccc gcgcgctgct gcgcggcgag
     5341 gccgaggtcg gttactcctg catgcgcccc tccggccacc acgccgaacc cgaccgcgcg
     5401 atgggcttct gcttcttcga caacgtcgcc atcgccgcgg cgatggcgat cgccgaccac
     5461 ggcctcgagc gggtgatggt gctcgattgg gacgtccacc acggcaacgg caccggcgag
     5521 atcttccgcc gccgcgacga cgtgctcttc ttcagcctgc accagtcgcc gctctatccc
     5581 ggcaccggcc ccgcctccga caagggctcg ggacccggcg agggcttcac ggtcaacctc
     5641 ccggtcgcgc ccggctccgg cgaggacgtc tggctcgggc tgatcgacga ggtcgtcatc
     5701 cccaccaccg agcgctaccg cccgcagctg atcctcgtct ccgccggcta cgacgcccac
     5761 cgcgaagacc cgctggcgag ctgcgccctc gagtcaacct cgttcgcggc gatggcgacc
     5821 cgcgtcggcg acctcgccgc ccgcctcggc gtgccgctcg gcctggtcca ggagggcggc
     5881 taccacgtgc cctcgctggt cgagtcgctg ctggcgacgc tgggcgccct cggcgcgcag
     5941 cccgccgagg cctgagctag cccagccgca gggtgatcgc ggtcgtcttc ttgaccgcgg
     6001 cgcccgagtc gggcttgaac ttcagcttcg gccgcagctt caccgagccg ttggcgagca
     6061 acgccagctt ggctgcgccc tttgccgcga gcgggagctt cagggtcttc ggccctttcg
     6121 cgctcttttt cgccacggcg acgcctttgc cggagagcgt cacggtcccg gcgccgggca
     6181 cctgcacctt cagcaccgcg gtgccggcgc gacggttcag cttcggcttg ccgagcacct
     6241 tgatcgtcgg ccgttgcacc ttcagcttgg cgagcgcggt ggccgcgcct ccgccggcgg
     6301 cagtcgcggt gatcgtcact tcgtagacgc ccggcttggc cccgttgggg acgttgacaa
     6361 cgacgctgcg ggtcgccgcc tcggcgcgga aggtcgcgcc ggcgaccgcg cccggggcgt
     6421 aggtgcgctg cggcatcgag atcga
//
LOCUS       DASRIG010000044         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2893224, whole genome shotgun sequence.
ACCESSION   DASRIG010000044 DASRIG010000000
VERSION     DASRIG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893224"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VFB52_01220"
     CDS             complement(<1..783)
                     /locus_tag="VFB52_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO04982.1"
                     /translation="MISKQEGEMGTVERNLDEVLVIDREEWPDGPPHALFKQMRGQCP
                     VHWTSHVPEFPESAGFWSITKADDIHAVSRDWETYSSEKGGIVAVTAGFPLELMRAMF
                     IGMDPPKHDRLKALFQAGFTPKRIAAHEEAIRAIVVDVLEGLEGRETCDLVTDVAQPV
                     VSRVIGNFMGIPPEDDAVWAQMMNAALGASDTDLNPDGVEGAIHKTVPEIFERCQKLI
                     AERRENPTDDLTSVLVHAEVEGEKLEEHEIVMGFFLLVAAGND"
     gene            complement(826..1863)
                     /locus_tag="VFB52_01225"
     CDS             complement(826..1863)
                     /locus_tag="VFB52_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04983.1"
                     /translation="MGRRIARIAVWIGGVALTIFLLDLVGVPVSEWIDDLFDKLGEIP
                     AWAIAGGIVLQSTQTALAALAWFGILRAALPEGGVTYRIVLASYATAVALNSFLPANI
                     GTWVMLLMFTTLFVGATFTMMLSGIFVQKIPFTVFNVALYLYLFLSVAGSFSIKLGFL
                     SDHTGLVVMIAIGAVVLIVLLGRIFWERLAGLRAQIAEGGAILRTPRRFLVDVVLPSA
                     GSYAARLAIVGVFLGAYGIPVTFHNVASVTASNSISNSVSATPGGVGVTQAMNTAALA
                     DSTNAATATAYSISQQLVTSAWNVVLAVVLVSWVFGWSGGRALVESSYADAKVKSREM
                     KDKRKRHGDEA"
     gene            1890..>2406
                     /locus_tag="VFB52_01230"
     CDS             1890..>2406
                     /locus_tag="VFB52_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04984.1"
                     /translation="MRARRSLSVLALALALLGLSAPAASAAAPKLERCDLGLPRPFQC
                     GRITVPMVRSDPALGTTRVAFAVRKRADTSRPSLGTIMAMDGGPGYGSTSAPFTRSLV
                     AVMAPLLRRHDLVVFDMRGTGRSQVLDCPDLQAGLVQESIAIGECANQLGPRYAGYTT
                     GEAAHDLDAVRR"
BASE COUNT          372 a          822 c          834 g          378 t
ORIGIN      
        1 gtcgttgccg gcggcgacga gcaggaagaa gcccatcacg atctcgtgct cctcgagctt
       61 ctcgccttcg acttcggcgt ggacgagcac gctggtcagg tcgtcggtcg ggttctcgcg
      121 ccgctcggcg atcagcttct ggcagcgctc gaagatttcc ggcacggttt tgtggatcgc
      181 gccctcgacg ccgtcggggt tgaggtcggt gtcgctggcg ccgagcgccg cgttcatcat
      241 ctgcgcccag accgcatcgt cctcgggcgg gatacccatg aagttgccga tcacccgcga
      301 gaccaccggc tgggcgacgt cggtgaccag atcgcaggtc tcgcggccct cgagcccctc
      361 gagcacgtcg acgacgatcg cccggatcgc ctcctcgtgg gcggcgatcc gcttcggcgt
      421 gaagccggcc tggaagagcg ccttgaggcg gtcgtgcttg ggcgggtcca tgccgatgaa
      481 catcgcccgc atcagctcca gcgggaagcc ggcggtgacc gcgacgatgc cgcccttttc
      541 ggaggagtag gtctcccagt cgcggctgac cgcgtggatg tcgtcggcct tggtgatcga
      601 ccagaacccg gccgattcgg ggaactcggg gacgtgcgag gtccagtgca ccgggcactg
      661 gccgcgcatc tgcttgaaca gcgcgtgggg aggaccgtcc ggccactcct cgcggtcgat
      721 cacgagtacc tcgtcgaggt tgcgttccac ggtccccatc tcgccctcct gcttcgaaat
      781 cacggtcgca gcgatcttaa ctggccagcc agccaggagg gaggctcacg cctcgtcacc
      841 gtggcgtttg cgtttgtctt tcatttcgcg gcttttcact ttcgcgtcgg cgtaggagga
      901 ctcgaccagg gctctgccgc cggaccagcc gaaaacccag gagacgagga cgactgcgag
      961 gacgacgttc caggccgagg tgaccagctg ctggctgatc gagtaggcgg tcgcggtggc
     1021 ggcgttggtc gagtcggcga gcgccgccgt gttcatcgcc tgcgtgaccc cgaccccgcc
     1081 cggcgtcgcc gacaccgagt tggagatcga gttcgaggcg gtcacgctgg cgacgttgtg
     1141 gaaggtgacc gggatcccgt aggcgccgag gaagacgccg acgatcgcca gccgcgccgc
     1201 gtagctgccc gccgagggca ggacgacgtc gacgaggaag cggcgcggcg tgcgcaggat
     1261 cgcgccacct tcggcgatct gggcccgcag gccggccagc cgctcccaga agatccgccc
     1321 cagcagcacg atcagcacca ccgccccgat ggcgatcatc acgaccagcc cggtgtggtc
     1381 ggagaggaag ccgagcttga tcgagaacga gccggcgacc gagaggaaca ggtagaggta
     1441 gagcgcgacg ttgaagacgg tgaacgggat cttctgcacg aagatcccgg agagcatcat
     1501 cgtgaaggtg gcgccgacga acagggtggt gaacatcagc agcatcaccc aggtgccgat
     1561 gttggcgggc aggaagctgt tcagcgcgac cgcggtcgcg taggaggcca gcacgatccg
     1621 gtaggtgacg cccccttccg gcagcgccgc acgcaggatc ccgaaccagg ccagcgccgc
     1681 cagcgcggtc tgggtgctct gcaggacgat cccgcccgcg atcgcccagg ccgggatctc
     1741 gccgagcttg tcgaagaggt cgtcgatcca ctcgctgacc ggcacgccga ccaggtcgag
     1801 caggaagatc gtgagggcga caccgccgat ccagaccgcg attcgagcga ttcgtcgtcc
     1861 cacggcgctc accctattgt tgggggacga tgagagcgcg tcgctcgctg tcggttctcg
     1921 ccctggcact ggccctgctc ggcttgtccg cgccggccgc atcggccgcg gcgcccaagc
     1981 tcgagcgctg cgacctcggc ctgccgcgcc ccttccagtg cggccggatc acggtgccga
     2041 tggttcgctc cgacccggcg ctcggaacca cccgcgtcgc cttcgcggtg cgcaagcggg
     2101 ccgacacctc gcgcccgtcg ctgggcacga tcatggcgat ggacggcggg cccggctacg
     2161 gctcgacctc ggcaccgttc acgcgctcgc tggtcgcggt gatggcgccg ttgctgcgcc
     2221 gccacgacct cgtcgtcttc gacatgcgcg ggaccgggcg ctcgcaggtg ctcgactgcc
     2281 ccgacctgca ggcgggcctg gtccaggaat cgatcgcgat cggcgagtgc gccaaccagc
     2341 tcggcccccg ctatgccggc tacacgaccg gcgaagccgc gcatgacctc gacgcggtgc
     2401 gtcgcg
//
LOCUS       DASRIG010000045         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1074695, whole genome shotgun sequence.
ACCESSION   DASRIG010000045 DASRIG010000000
VERSION     DASRIG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074695"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFB52_01235"
     CDS             complement(<1..224)
                     /locus_tag="VFB52_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04985.1"
                     /translation="MASATTFEFRAMDLSGTTSSGELEADSKAAVGEQLRQRGLIVLD
                     VSEKSNPFNIEDLFKGWKSVDKRELAIFSR"
     gene            complement(225..1382)
                     /locus_tag="VFB52_01240"
     CDS             complement(225..1382)
                     /locus_tag="VFB52_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZO04986.1"
                     /translation="MSLPDFDFSDVLARMVEVRASDVHITAGFPPAIREKGAIHPMEG
                     FPVLNGQQTREIVYGILNDDQRKRFENNQQLDFAYAIPGVARFRVNCFFQRGSVSAAF
                     RLVPQEIPALDSLGVPQVLRDLTRKPRGFVLVTGPTGSGKSTTLAAMIDVINQEREDH
                     ILTIEDPIEFLHRHKRSIVNQREIGSDAPDFSLGLRAALRQDPDVILVGEMRDLETIS
                     TALTAAETGHLVFATLHTQSTSQTVDRIIDVFPSQQQGQVRTQLSIALQGIVTQQLLP
                     TIDGKGRTVATEVLIPTPAVRNLIREGKTHQIYGALQTSGAVGMQTMDADLARLVRTG
                     KITRSLAEQRASVPEELGRLLGGPSADGAVPGNGAGAPAAQPMYTQPGGGA"
     gene            complement(1369..>2501)
                     /locus_tag="VFB52_01245"
     CDS             complement(1369..>2501)
                     /locus_tag="VFB52_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZO04987.1"
                     /translation="VYSVLAQAVGEGASDVHLEPEEGELRVRFRVDGVLREAAHVPKR
                     MTGAVISRLKIMSELDIAEKRVPQDGRVSVAVEERRIDLRVTTLPTQRGEGATIRILD
                     ESNSQRSLDDLGMDGSARAVFEHSFRQAYGAVLVTGPTGSGKSTTLYAALGELNDVEK
                     KIITIEDPVEYRIDGVNQINVNRKAGLDFATGLRSILRADPDIVMVGEIRDAETARIA
                     IEAALTGHMMLTTLHTNDAPGAIARLTEMGIETFLTASAVDCVVAQRLARRLCPSCKQ
                     RAVIPKAALAEAGVPVYADIEAYEPAGCGHCGQSGYRGRVGIYSVMNLTEKLKEMTIG
                     NAAETEIAATAREEGMLTLREDGVTKVRGGQTSLEEVLRVTA"
BASE COUNT          346 a          853 c          870 g          432 t
ORIGIN      
        1 tggcgcgaga agattgccag ctcgcgtttg tcgacgctct tccagccttt gaagaggtcc
       61 tcgatgttga acgggttcga cttctcgctg acgtcgagca cgatcagccc gcgctggcgc
      121 agctgctcgc cgaccgcagc cttcgagtcg gcctccagct cgccgctcga ggtcgtcccc
      181 gagaggtcca tcgcgcgaaa ctcgaaggtc gtcgccgagg ccatctacgc cccaccgccc
      241 ggctgcgtgt acatcggctg ggccgcgggc gcgcccgcgc cattgccggg aactgcgcca
      301 tccgccgacg gcccgccgag cagccggccc agctcctccg ggaccgaggc ccgctgttcg
      361 gccagcgagc gcgtgatctt gccggtgcgc acgagccgcg ccagatcggc gtccatcgtc
      421 tgcatcccga ccgcgccgga ggtctgcagg gcgccgtaga tctggtgcgt tttgccctcg
      481 cggatcaggt ttcgcaccgc cggcgtcggg atcagcacct cggtggcgac cgtgcgacct
      541 ttgccgtcaa tcgtgggcag cagctgctgg gtgacgatgc cctgcagggc gatcgagagc
      601 tgggtacgca cctggccctg ctgctgggag gggaagacgt cgatgatccg gtcgaccgtc
      661 tgcgaggtcg actgggtgtg cagggtggcg aagaccaggt ggccggtctc ggccgcggtc
      721 agcgcggtcg agatcgtctc caggtccctc atctctccga ccaggatcac gtcggggtcc
      781 tgccgcaacg cggcccgcag cccgagcgag aagtcaggcg cgtcggagcc gatctcgcgc
      841 tggttgacga tcgagcgttt gtgccggtgc aggaactcga tcgggtcctc gatcgtgagg
      901 atgtggtcct cgcgctcctg gttgatcaca tcgatcatcg ccgccagggt cgtgctcttg
      961 cccgagccgg tcgggccggt gacgaggacg aagccgcgcg gtttgcgggt caggtcgcgc
     1021 agcacctgcg gcacgccgag cgagtcgagc gccgggatct cctgcgggac gagccggaag
     1081 gcggcgctga ccgagccgcg ctggaagaag cagttgacgc ggaagcgggc gacgccggga
     1141 atcgcgtagg cgaagtcgag ctgctggttg ttctcgaacc gtttgcgctg atcgtcgttg
     1201 aggatcccgt agacgatctc ccgcgtctgc tgcccgttca gcaccgggaa gccctccatc
     1261 gggtggatcg cgcccttttc ccggatcgca ggtgggaagc ccgcggtgat gtggacgtcg
     1321 gacgcgcgca cctcgaccat ccgggcgagc acgtcggaga agtcgaaatc aggcagtgac
     1381 acggagcacc tcctcgaggc tggtctggcc gccgcgcacc ttggtcacgc cgtcctcgcg
     1441 cagggtcagc atcccctctt cacgggcagt ggcggcgatt tcggtctcag cggcgttgcc
     1501 gatcgtcatt tctttgagct tttcggtcag gttcatcacc gagtagatcc cgacccggcc
     1561 gcggtagccg ctctggccgc agtgcccgca gccggccggt tcgtaggcct cgatgtcggc
     1621 gtagaccggc acgccggcct cggccagggc cgctttcggg atcaccgccc gctgcttgca
     1681 gctcgggcag agccggcggg caagccgctg ggcgaccacg cagtcgactg ccgaggcggt
     1741 gaggaaggtc tcgatcccca tctcggtgag ccgcgcaatc gcgccggggg cgtcattggt
     1801 gtgcagcgtg gtcagcatca tgtggccggt cagcgcggcc tcgatcgcga tccgcgcggt
     1861 ctccgcgtcg cggatctcgc cgaccatcac gatgtccggg tcggcgcgca ggatcgagcg
     1921 cagcccggtg gcgaagtcga ggcccgcctt gcggttgacg ttgatctggt tgaccccgtc
     1981 gatccggtac tcgaccgggt cctcgatcgt gatgatcttc ttctcgacgt cgttgagctc
     2041 gcccagcgcc gcgtagagcg tcgtgctctt gccggagccg gtcgggccgg tgaccaggac
     2101 cgcaccgtag gcctggcgga aggagtgctc gaagaccgcc cgagccgagc cgtccatgcc
     2161 gaggtcgtcg agcgagcgct gcgagttgct ttcgtcgagg atgcggatcg tcgccccctc
     2221 gccgcgctgg gtcggcagcg tggtcacacg caggtcgatg cggcgctcct cgaccgcgac
     2281 gctgacgcga ccgtcctgcg gcacccgttt ctcggcgatg tcgagctcgc tcatgatctt
     2341 caggcgcgag atcaccgcgc cggtcatccg cttcggcacg tgcgcggctt cgcgcagcac
     2401 cccgtcgacc cggaaccgca cccgcagctc gccctcttcg ggctccaggt gcacgtcgga
     2461 ggccccctcc cccaccgcct gggcgaggac gctgtagacc a
//
LOCUS       DASRIG010000046         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3413985, whole genome shotgun sequence.
ACCESSION   DASRIG010000046 DASRIG010000000
VERSION     DASRIG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3413985"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VFB52_01250"
     CDS             <1..1863
                     /locus_tag="VFB52_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO04988.1"
                     /translation="VAEACGALGRSEILRPEPPVAFVHSLVREAIYQDLSVGERELHH
                     ERAARALTAAGATPEQVATHLLHVPRRGQEWVVETLRAAAGSAVAKGAPESAVAYLKR
                     ALEEPPPGALEANVLFELGSAEASTSGPDAIVHLRAARAGLADEEKRAVAANVLIRNM
                     LVAGSPSAAAEVAREAAAELPPQLERMGRSFEALAQAAVFFGVGEFTELDRLDSLQPP
                     PADAPVFDLLLAAMAAYTWTYRCRPAEECAAMALASHARDLEILTADNSVISVAALMP
                     LAYADREEALSGWDASAAEAHRRGSTFDISGVHLWRGATLLQRGELPEAAADLRAAVE
                     DQRVFGSSEIPRSYTAGFLASALLAQGDVAGAWAALGDGYESAAGTDGRRFWLESRLE
                     LLLAEGRDEEVLRIADDLRDAYAWALNPVVARWRSGKARALDRLGRGEEALAVLAEEL
                     ELARAYGAPGTVGRVLRELGALEREAGLERLEEAVAVLDGPPCRLELAKALGAQGAAL
                     RLARKPKEARDPLRRALELAEACGAAPLEAHVRSELQAAGVRPRSTALSGVESLTASE
                     KRVAGLAADGGTNRDIAQELFVTPKTVEVHLSNTYRKLGISSRRQLPAALAAVG"
     gene            1868..2140
                     /locus_tag="VFB52_01255"
     CDS             1868..2140
                     /locus_tag="VFB52_01255"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZO04989.1"
                     /translation="MPPQQSKPVNWRAWILGTIVVLVLVVILQNSQEVSVEVLFASFN
                     APLIVVLLIAVGVGAGLGYAAPILLRHRREQRRENKAIEAKSTPKR"
     gene            complement(2187..2714)
                     /locus_tag="VFB52_01260"
     CDS             complement(2187..2714)
                     /locus_tag="VFB52_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04990.1"
                     /translation="MEFQGIDRFEVDFIGDFFDGLEMIRGRENAFLVQCSAHPLVHKI
                     TEKHWRDVFVVDTFIYPTKALAVLSRREVERPRTLGLVPATAGYIDADDWEEIIDVQS
                     KPIVAEELLAGRYEAGLTHLEHLALHPDELRLDLEIGEIDTTWVVYGTRKRFQGEVIG
                     IPSAEIFGQVEPALS"
BASE COUNT          449 a         1143 c         1106 g          465 t
ORIGIN      
        1 gtcgccgagg cctgcggcgc gctcggccgc tcggagatcc tccggcccga gccgccggtc
       61 gccttcgtcc actcgctggt ccgcgaggcg atctaccagg acctctcggt cggcgagcgc
      121 gagctgcacc acgaacgcgc ggcccgggcg ctgaccgcgg ccggagccac ccccgagcag
      181 gtcgcaacgc acctgctgca cgtgccgcgc cgcggccagg agtgggtggt ggagacgctg
      241 cgtgcggcgg ccggctcggc ggtcgccaag ggcgcgcccg aaagcgcagt cgcctacctg
      301 aagcgggcgc tggaggagcc gccgccgggg gcgctggagg cgaacgtgct gtttgagctc
      361 ggctcggccg aggcgagcac ctccgggccc gacgcgatcg tgcacctgcg cgccgcgcgc
      421 gccgggctcg cggatgagga gaagcgggcg gtcgccgcca acgtcctgat ccgcaacatg
      481 ctggtcgccg gctcgcccag tgccgccgcc gaagtcgccc gcgaggccgc cgccgagctg
      541 ccgccccagc tggagcggat gggtcgcagc ttcgaggcac tcgcccaggc agcggtcttc
      601 ttcggggtcg gtgagttcac cgagctcgac cggctcgact cgctgcagcc cccgcccgcc
      661 gacgctccgg tcttcgacct gctgctggcg gcgatggctg cctacacctg gacctaccgc
      721 tgccggcccg ccgaggagtg cgcggcgatg gcgctcgcct cgcacgcgcg agacttggag
      781 atcctcaccg ccgacaacag cgtgatctcg gtcgcggcgt tgatgcccct cgcctacgcc
      841 gaccgcgagg aggcgctgag cggctgggac gcttcggccg ccgaggccca ccgccgcggc
      901 tccaccttcg acatctccgg tgtccacctc tggcgcggcg cgacgctgct gcagcggggc
      961 gaactgccgg aggcggcggc cgacctgcgg gcggcggtcg aagaccagcg cgtgttcggc
     1021 tcctcggaga tcccgcgcag ctacaccgcc ggcttcctcg cctccgccct gctcgcgcag
     1081 ggcgacgtgg ccggcgcctg ggcggcgctc ggcgatggct acgagtcggc ggccggcacc
     1141 gacgggcggc gcttctggct cgaaagccgg ctcgagctgc tgctcgccga gggccgcgac
     1201 gaggaggtcc tgaggatcgc cgacgacctc cgcgacgcct acgcctgggc gctcaacccg
     1261 gtcgtcgccc gctggcgcag cggcaaggcg cgcgcgctcg accggctcgg ccgcggcgag
     1321 gaggcgctgg cggtgctcgc cgaggagctg gagctggccc gcgcctacgg ggcgccgggc
     1381 accgtcggcc gggtcctgcg cgagctcggc gcgctcgaac gcgaggccgg gctggagcgc
     1441 ttggaggagg cggtcgcggt gctcgacggg ccgccctgcc ggctcgagct ggccaaggcg
     1501 ctcggcgcgc agggagcggc cctgcgcctc gcccgcaagc cgaaggaggc ccgcgatccc
     1561 ctgcgacgcg cgctcgagct ggccgaggcc tgcggggccg cgccgctgga ggcgcacgtg
     1621 cgctccgagc tgcaggccgc cggcgttcgt ccccgcagca ccgctctgtc gggcgtggag
     1681 tcgctgacgg cgagcgagaa gcgggtcgcc ggcctcgccg ccgatggcgg caccaatcgc
     1741 gacatcgccc aggagctgtt cgtgaccccg aagacggtcg aggttcacct cagcaacacc
     1801 taccgcaagc tcgggatcag ctcgcgccgc cagctgcccg ccgcgctggc ggccgtagga
     1861 tgagcgggtg ccaccacagc agtccaagcc ggtcaactgg cgcgcctgga tcctcggcac
     1921 gatcgtcgtc ctggtcctcg tcgtcatcct gcagaactcg caggaggtca gcgtcgaggt
     1981 cctcttcgcc agcttcaacg cgccgctgat cgtcgtcctc ctgatcgccg tcggggtcgg
     2041 cgccgggctc ggctacgccg ccccgatcct gctccgccac cgccgcgagc agcggcgcga
     2101 gaacaaggcg atcgaggcga agtcgacgcc gaagcgctga ttcgcccgcc gcgtcttagc
     2161 gcccccgtcc ccgctccccg gggacgctaa gacagggcgg gttccacctg cccgaagatc
     2221 tcggccgagg ggatcccgat cacctcgccc tggaaccgct tgcgggtccc gtacaccacc
     2281 cacgtggtgt cgatttcgcc gatctcgaga tcgagccgca gctcgtccgg gtgcagcgcc
     2341 aggtgctcga ggtgggtgag cccggcctcg tagcgcccgg cgagcagctc ttcggcgacg
     2401 atcggcttcg actgcacgtc gatgatctcc tcccagtcgt ccgcgtcgat gtagccggca
     2461 gttgcaggta cgaggccgag cgtcctgggc cgctcgacct cgcggcggct gagcaccgcc
     2521 aaagccttgg tcgggtagat gaaggtgtcg acgacgaaga cgtcgcgcca gtgcttctcg
     2581 gtgatcttgt ggaccagcgg atgagcgctg cactggacta ggaaggcgtt ctccctcccc
     2641 cggatcatct ccagcccgtc gaagaagtcg ccgatgaaat cgacctcgaa ccgatcgatt
     2701 ccctggaact ccatgtaggc gcggcaggcc cgctcgtggc acgttccggt cggtcctaga
     2761 gttaccaaca agggggccga atttcgcctt ctgtcctcca ttcccatgga cgaggctaat
     2821 cgactaaaca aaaatgtcaa cgtttcagac actcgtctgc gaaccccttt gattgaactc
     2881 gcctcagtgg tataactcaa attgagttga ccttgtccag gtccaaccga aagggagaaa
     2941 ctggggtgcg agctggggct caggcgctgt cgcttcttgc ggtgccgctg aacttccacg
     3001 ttctcacggc gctagccgag ggaccaaggc cattggtgga tctccgccgc gaagcgggat
     3061 ctcctcccca gaccacgatg cgcggtcacc tgcgcaaact ggcggagctc gaagtcgtcg
     3121 aacggcgtcg cgaaagtggc ttccccggct cggtggacta cca
//
LOCUS       DASRIG010000047         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1333420, whole genome shotgun sequence.
ACCESSION   DASRIG010000047 DASRIG010000000
VERSION     DASRIG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333420"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="hemB"
                     /locus_tag="VFB52_01265"
     CDS             complement(<1..869)
                     /gene="hemB"
                     /locus_tag="VFB52_01265"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZO04991.1"
                     /translation="MAFPATRMRRLRRTDTLRGLVRETHLGPEHLIQPIFVVAGEGVR
                     EEVPSMPGIERFSINNLVEEAGEIAAAGVGAVMLFGVPGAKDEVGSGAYDDEGIVQMA
                     VRALKEAHTDLTVITDVCLCEYTSHGHCGIIGPDGQVENDISVELLAKTAISHAEAGA
                     DAVAPSDMMDGRIGSIRFQMDEEGHPDVPIIAYSAKYASAFYGPFRDAADSAPAFGDR
                     RSHQMDPANLVEALREVALDVEEGADIVMVKPAMTYLDVIARVKEEFGLPTAAYHVSG
                     EYSMLKAAARNGW"
     gene            939..1643
                     /locus_tag="VFB52_01270"
     CDS             939..1643
                     /locus_tag="VFB52_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO04992.1"
                     /translation="MQAQGLVKRYGEREALRGVSFSAAPGELVAVIGPNGAGKTTLLS
                     ILAGILAADAGAVDVEGGAVGWVPQQAALYRRLTVEENLLLFARLERHQDPRASVEQM
                     LELTGLGERRGEIVARLSGGNQQRINIAIGLLARPRVLLLDEPSVGLDPRQRARLWEF
                     VSGLADDGTTVIFSTHDIQEAERYGGRLLVLADGESLFDGSSAALREAVRREAPEAAD
                     RDFETAFVAYLHHRGH"
     gene            1643..2992
                     /locus_tag="VFB52_01275"
     CDS             1643..2992
                     /locus_tag="VFB52_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO04993.1"
                     /translation="MRWLLLKDLQILRRSPLQAVLLVVYPILIAVLVGFAISGDDEKP
                     KVAFLNEVPADSRISVGGEQLPSSNVSERICRRVECVDVASREEAEAKVSSGEVLAAL
                     VLPANLVDRINSLSTLTPGVPEVEVLVSEDNSVKAGLVDDRIDALLAEANLAVARRIA
                     KEGSRYLGLVINGGKVELIGQTFPILGLRASAEILAALEPALEPQLRPALKQVREFAD
                     EARDNLDVAGPLIDRLAQPIAVKKVAVGGEAPPLETFAIAVAATFTLAFVTVLLVAGS
                     LALEREENAFPRLTRGLVSREALLVEKVLLGVAVGLVVTLLLLAGLAIFAPLAWERFG
                     LWLAAIVLGAAALAAAGAALGAAAREVRAVALLAFMVTLPIAFLSLIPSGAVGSGLYD
                     VIGVVTALFPFKPTLEAITAALDSTGDGIGLPLLHLAVLIAAYGLLARLALRRFASV"
     gene            complement(2994..3695)
                     /locus_tag="VFB52_01280"
     CDS             complement(2994..3695)
                     /locus_tag="VFB52_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04994.1"
                     /translation="MPKKEREPIFVLDESEKFQPLAVESVEAAPAKLVGLDGKEHGNV
                     ELAKLPPQGGRMNFPPDPEELEKRLRTAFGGVGYKRELVAGGLTWVQYWLWYLHNPKQ
                     IVVAGVHEGDWEFVQVGYHGEAPICVTVSRHRTAGARMWWDVERRDGRPVVYVARDSH
                     AHYFTPVSEAFEWEDDADGKGEELALEWREFGDWATWPGLWGNSTGLGRSPQSPGSQA
                     ERWNAPHRYHSSGSR"
     gene            3790..4245
                     /locus_tag="VFB52_01285"
     CDS             3790..4245
                     /locus_tag="VFB52_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO04995.1"
                     /translation="MSDAGPLLIAYDGSSYSKAAIADAGRQLQGWRHAVVLTVREPLE
                     QVPFMSTLGASVDAGTFAALQKSAADEAAGVAEEGAELARAAGFEAEARVEVGQTPWE
                     RIVEVGEELDAGLIVIGSRGLTGLKRAALGSVAAAVAQHSGRSVLIVHS"
     gene            complement(4256..4957)
                     /locus_tag="VFB52_01290"
     CDS             complement(4256..4957)
                     /locus_tag="VFB52_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04996.1"
                     /translation="MAGPEQPGEILARAAAYPYDAPLHSFVQTGTQTREARAEGLDLS
                     EREPLLAYGSNAAPAVLARKLATLPGEPLPVLRAALADFDVVYSAHISPYGAVPATLQ
                     RSPGTTAPVFVAYPTAAQRRLLTATEPNYELRELAGLTLRTELPDPLAAVHAYVSRHG
                     CLAIADSEVALAAVAADGRRFPAMGEVEVLERVRHLLAPELDLERFVESSLDPGMAAA
                     RTTVLRGNAITLRSP"
     gene            4987..>5655
                     /locus_tag="VFB52_01295"
     CDS             4987..>5655
                     /locus_tag="VFB52_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZO04997.1"
                     /translation="MRVLFIGDVVGGPGRRGLRETMPSLRERYAPDLVIVNGENSAGG
                     IGITERTANDIFGAGAGVITSGNHVYRHREAYEFLDRDERVIRPANYPSANPGRGHTV
                     VEAGGMRVGVINLSGGVGLRVARSPFDTVDGILERIEADAVIVDFHAEVTSEKVAMGW
                     HLDGRAAAVFGTHTHVPTADGRVLPKGTAFISDVGMTGSRTSVLGVKPEQALASLITQ
                     MPARF"
BASE COUNT          777 a         2014 c         2021 g          843 t
ORIGIN      
        1 atccagccgt tgcgcgctgc cgccttgagc atcgagtatt cgccgctcac gtgatacgcg
       61 gccgtcggca ggccgaactc ctccttcacc ctcgcgatca catccagata cgtcatcgcc
      121 ggcttgacca tgacgatgtc ggccccttcc tcgacgtcca gcgccacttc gcgcagcgcc
      181 tcgacgaggt tggcgggatc catctgatgc gaccggcggt cgccgaacgc gggcgccgaa
      241 tcggcggcgt cgcggaacgg cccgtagaag gccgaggcgt acttggcgct gtaagcgatg
      301 atcggcacgt cggggtggcc ctcctcgtcc atctggaagc ggatcgagcc gatccggccg
      361 tccatcatgt cgctgggcgc gaccgcgtcg gcgccggcct cggcgtgcga gatcgcggtt
      421 ttcgccagca gctcgaccga gatgtcgttt tcgacctggc cgtcggggcc gatgattccg
      481 cagtggccgt gcgaggtgta ctcgcagaga cagacgtcgg tgatcacggt caggtccgtg
      541 tgcgcctcct tcagcgcccg caccgccatc tgcacgatcc cctcgtcgtc gtaggcgccg
      601 ctgccgacct cgtccttggc gccgggcacg ccgaagagca tgaccgcgcc gactccggcg
      661 gcggcgatct cgccggcctc ctcgaccagg ttgttgatcg agaagcgctc gatgccgggc
      721 atcgagggca cctcctcgcg cacgccctcg ccggcgacga cgaagatcgg ctggatcagg
      781 tgctcggggc cgaggtgcgt ctcacgaacc aggccccgca gggtgtcggt gcgccgtagc
      841 cggcgcatcc gcgttgcggg aaaagccatc gcgaccgagt ttagggaagg gataaggaat
      901 tccccctcct tcatacgccg gcagtagcgt tcccgcgggt gcaggcacaa gggttggtca
      961 agcggtatgg cgagcgggaa gcgctgcggg gggtcagctt ctccgccgcc ccgggcgagc
     1021 tggttgcggt catcggcccg aacggagccg ggaagacgac gctgctctcg atcctcgccg
     1081 ggatcctcgc ggcggacgcg ggcgcggtcg acgtcgaggg cggggcggtt ggctgggtgc
     1141 cccagcaggc ggcgctctac cgacgcctga ccgtggagga gaacctgctg ctgttcgccc
     1201 gcctggagcg gcaccaggac ccacgcgcct cggtcgagca gatgctcgaa ctgaccggcc
     1261 tcggcgagcg ccgcggcgag atcgtcgccc gcctctcggg cggcaaccag cagcggatca
     1321 acatcgcgat cgggctgctg gcgcggccgc gggtgctgct gctcgacgag cccagcgtcg
     1381 ggctcgaccc gcgccagcgc gcccgtctct gggagttcgt ctccggactc gccgacgacg
     1441 gcaccacggt gatcttctcg acccacgaca tccaggaggc cgagcgctac ggcggtcgcc
     1501 tgctggtgct cgccgacggc gagtcgctgt tcgacggctc ctccgctgcg ctgcgtgagg
     1561 cggtgcggcg cgaggccccg gaggccgccg accgcgactt cgagaccgcc ttcgtcgcct
     1621 acctgcacca ccggggtcac tgatgcgctg gctgctgctc aaggacctgc agatcctccg
     1681 ccgctcgccg ctgcaggcgg tgctgctggt cgtctacccg atcctgatcg cggtcctggt
     1741 cgggttcgcg atctccggcg acgacgagaa gccgaaggtc gccttcctca acgaggtgcc
     1801 ggccgacagc cggatcagcg tcgggggcga gcagcttccc tcctccaacg tcagcgaacg
     1861 gatctgccgg cgggtcgagt gcgtcgacgt cgccagccgc gaggaggccg aagcgaaggt
     1921 cagctccggc gaagtgctgg cggcgctggt cctgcccgcc aacctggtcg accggatcaa
     1981 ctcgctctcg accctgaccc ccggcgtccc ggaagtcgaa gtgctcgtca gcgaggacaa
     2041 ctcggtcaag gccggcctgg tcgacgaccg gatcgacgcc ttgctggccg aagcgaacct
     2101 ggcggtcgcg cggcgcatcg ccaaagaagg cagccgctac ctggggctgg tgatcaacgg
     2161 cggcaaggtc gaactgatcg gccagacctt cccgatcctc gggctgcggg ccagcgccga
     2221 aatcctcgcc gcgctcgaac cggcgctgga accgcagctg cggccggcgc tgaaacaggt
     2281 ccgcgagttc gccgacgaag cgcgcgacaa cctcgacgtc gccggaccgc tgatcgaccg
     2341 gctggcgcag ccgatcgcgg tgaagaaggt cgcggtcggg ggtgaggcac cgcccctgga
     2401 aacctttgcg atcgccgtcg ccgccacctt cacgctcgcc ttcgtgaccg tgctgctggt
     2461 cgccggatcg ctcgcgctcg agcgcgagga gaacgccttc ccgaggctca cgcgcgggct
     2521 cgtctcgcgt gaggcgttgc tggtggagaa ggtcctgctc ggggtcgcgg tcggactcgt
     2581 cgtcaccctg ctgctgttag ccgggctggc gatcttcgcg ccgctggcct gggagcggtt
     2641 cggcctctgg ctggccgcga tcgtgctcgg cgcggcggcg ctggccgccg ccggggcggc
     2701 gctcggcgcc gcggcgcgcg aggtccgcgc cgtcgccctg ctcgccttca tggtcacgct
     2761 gccgatcgcc ttcctctcgc tgatcccctc cggcgcggtc ggctccggcc tctacgacgt
     2821 gatcggcgtc gtcaccgcgc tcttcccgtt caagccgacc ctggaagcga tcaccgcggc
     2881 gctcgactcg accggggacg ggatcggatt gccgctgctg cacctggcgg tgctgatcgc
     2941 cgcctacggg ctgctggcgc ggctggcgct gcgccgcttc gcttcggtct gagtcagcga
     3001 ctgccgctgg agtggtagcg gtgcggcgcg ttccagcgct ccgcttggct gcccggcgac
     3061 tgcggtgagc ggccgaggcc ggtcgagttc ccccagaggc cgggccaggt cgcccagtcg
     3121 ccgaactcgc gccactcgag cgccagctcc tcgcctttgc cgtcggcgtc gtcctcccat
     3181 tcgaacgctt ccgagaccgg ggtgaagtag tgggcgtgcg agtcgcgggc gacgtagacg
     3241 accggtcgcc cgtcgcggcg ctcgacgtcc caccacatgc gcgcgccggc ggtgcggtgg
     3301 cggctgacgg tgacgcagat cggcgcctcg ccgtggtagc cgacctggac gaactcccag
     3361 tcgccctcgt gcaccccggc gacgacgatc tgcttcgggt tgtgcaggta ccagagccag
     3421 tactgcaccc aggtcagccc gccggcgacc agctcgcgct tgtagccgac cccgccgaag
     3481 gcggtgcgca ggcgtttctc cagttcctcc gggtcgggcg ggaagttcat ccgcccgccc
     3541 tgcggcggca gtttcgccag ctcgacgttg ccgtgctctt tgccgtcgag cccgaccagt
     3601 ttcgccggcg ccgcctcgac gctctcgacc gcgagcggct gaaacttctc gctttcgtcg
     3661 aggacgaaga tcggctcgcg ctcttttttc ggcatggtca gcgcgaacct agcgctcagc
     3721 aacttccggg gcaggaacac cgcgttacgg agatcggccg gcgaggccga cccggtacgg
     3781 tccagggcga tgtcggacgc cggcccactg ctgatcgcct acgacggctc ttcgtactcg
     3841 aaggccgcga tcgccgacgc gggccgccaa ctgcagggct ggcgccacgc ggtcgtgctg
     3901 acggtccgcg agccgctcga gcaggtgccg ttcatgagca cgctcggcgc ctcggttgac
     3961 gccggcacct tcgcggccct ccagaaaagc gccgcggacg aggcggccgg ggtcgccgag
     4021 gaaggcgcgg agctggcccg ggcggccggg ttcgaggctg aggccagggt cgaggtggga
     4081 cagacgccgt gggagcggat cgtcgaggtc ggcgaggagc tggacgccgg gctgatcgtg
     4141 atcggctcac gcgggctgac cggcctcaaa cgcgcagcgc tcggcagcgt cgccgcagcg
     4201 gtcgcccagc acagcggtcg cagcgtcctg atcgtccact cctgagccca gcccgctaag
     4261 gcgagcgcag cgtgatcgcg ttgccgcgca ggaccgtggt ccgcgccgcg gccatccccg
     4321 gatcgaggct cgactcgacg aaacgctcga ggtcgagctc gggcgccagc aggtggcgga
     4381 cccgctccag cacttcgacc tcgcccatcg ccgggaagcg ccggccgtcc gccgcgaccg
     4441 cggccagggc cacctcggag tcggcgatcg cgagacagcc gtggcggctg acgtaggcgt
     4501 ggaccgcggc cagcgggtcg ggcagctcgg tgcgcagggt gaggccggcg agctcgcgca
     4561 gctcgtagtt cggctcggtc gcggtcagca gccggcgctg ggcggcggtc gggtaggcga
     4621 cgaagaccgg cgcggtcgtc cccgggctgc gctgcagggt cgcgggcacg gcgccatagg
     4681 gggagatgtg ggccgagtag acgacgtcga agtcggcaag cgcggcgcgc agcacgggca
     4741 gtggctcgcc cggcaacgtc gccagcttgc gcgccagcac cgccggcgcg gcgttcgacc
     4801 cgtaggcaag caacggctcc cgctcgctca ggtcgagccc ctcggcgcgc gcctcgcggg
     4861 tctgcgtccc ggtctgaacg aagctgtgca ggggcgcgtc gtagggatag gcggcggcgc
     4921 gcgccaagat ctcgcccggt tgctcgggtc cagccaagac ccgaatgtcg cagatagcat
     4981 cgtccgatgc gcgtcctttt catcggcgac gtggtcgggg gtcccgggcg acgggggctg
     5041 cgcgagacga tgccgagcct gcgcgagcgc tatgccccgg acctggtgat cgtcaacggc
     5101 gagaactcgg ccggcgggat cgggatcacc gagcgcaccg ccaacgacat cttcggcgcg
     5161 ggcgccggcg tgatcaccag cggcaaccac gtctaccgcc accgcgaggc ctacgagttc
     5221 ctcgaccgcg acgagcgcgt gatccgcccc gccaactacc cgagcgccaa ccccggccgc
     5281 ggccacaccg tggtcgaggc gggcgggatg cgggtcggcg tgatcaacct cagcggcggg
     5341 gtcgggctgc gggtcgcgcg ctcgcccttc gacaccgtcg acgggatcct cgagcggatc
     5401 gaggccgacg cggtgatcgt cgacttccac gccgaggtga ccagcgagaa ggtggcgatg
     5461 ggctggcacc tcgacggccg cgccgcggcg gtcttcggca cccacaccca cgtccccacc
     5521 gccgacggcc gcgtgctccc caagggcacc gccttcatca gcgacgtcgg catgaccggc
     5581 tcacgcacca gcgtgctcgg ggtcaagccc gagcaggcgc tggcctcgct gatcacccag
     5641 atgccggccc gcttc
//
LOCUS       DASRIG010000048         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_545384, whole genome shotgun sequence.
ACCESSION   DASRIG010000048 DASRIG010000000
VERSION     DASRIG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545384"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="coaBC"
                     /locus_tag="VFB52_01300"
     CDS             <1..997
                     /gene="coaBC"
                     /locus_tag="VFB52_01300"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZO04998.1"
                     /translation="LELVANADLFMVAPASANTVAKLAAGIADSMLTTSFLACTAPRL
                     VAPAMNDRMYSDAATQANLSTLRERGVEVIEPGEGKLASRGEHGRGRLPDPAELLARV
                     EAVLPSGARPWDGLKVLVTAGGTREPIDPVRFIGNRSSGRMGIALAAAAARRGAEVTL
                     IAANVGLPEPAGVRRVDVETAAELAAAAAAEFPAAHLLLMAAAPADFRAAAPADQKMV
                     RADGLELRLEPTEDILAGLTAQRAPGQTIVGFAAEHGGDAVGRAREKRARKGVDMIVL
                     NDVSNPEIGFESTRNAVTLVDAEGETEVAIASKDAIADAILDHCERLQEKAGIAE"
     gene            1086..1346
                     /locus_tag="VFB52_01305"
     CDS             1086..1346
                     /locus_tag="VFB52_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO04999.1"
                     /translation="MTDLQQSIQEQLADLDSELDVVALERPGNETLRIFIDHPGGVDL
                     ALCERVTKHLNHLLVDYSLEVSSPGPKYRRSSTSSDTKESPQ"
     gene            1343..>2275
                     /gene="nusA"
                     /locus_tag="VFB52_01310"
     CDS             1343..>2275
                     /gene="nusA"
                     /locus_tag="VFB52_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZO05000.1"
                     /translation="MTKEIVDAVKALEQEKGISADKLMDALEDALLSAYKKTPGAAKY
                     ARVDLDHETGDFRVYELILPPELEEKLLAEAAEQQDEPEVDPETGELKEPEDPELDPE
                     MLAPYESQITTKDVTPEDFGRIAAQTAKQVILQRIREAERQMMYDEYQDRVGELITGI
                     IQQSDKRYTLVQLRERVEALLPKSEQVYNERYDHGMRVKAVITDVSDSTKGPAIVVSR
                     RSPELIKSLFELEVPEIADKLVEIVGVAREPGYRSKIAVVPHADGVDPVGACVGPRGS
                     RVRMVVSELRGEKIDIIPYNEEPARFVAKALSPAR"
BASE COUNT          394 a          799 c          749 g          333 t
ORIGIN      
        1 cctcgagctg gttgccaacg ccgacctgtt catggtcgcg cccgcgtccg cgaacacggt
       61 cgcaaagctc gcggccggaa tcgccgactc gatgctgacc acctcgttcc tcgcctgcac
      121 ggcgccacgc ctggtcgcgc cggcgatgaa cgaccgcatg tattccgatg cggcgaccca
      181 ggcgaacctg tcgaccctgc gcgagcgcgg ggtcgaggtg atcgaacccg gcgagggcaa
      241 gctcgcttcg cgcggcgagc acggccgcgg ccgcttgccc gatcccgccg agctgctcgc
      301 ccgggtcgag gccgtgctgc cgtccggcgc ccggccctgg gacgggctca aggttctggt
      361 caccgcgggt ggcacccgcg agccgatcga cccggttcgc ttcatcggca accgctccag
      421 cgggcgcatg gggatcgccc ttgccgccgc cgcggcgcgg cgcggggcgg aggtgaccct
      481 gatcgccgcc aacgtcgggc tgcccgagcc agcgggggtg cgccgtgtcg acgtcgagac
      541 cgccgcagag ctggccgcgg ccgccgccgc cgagttcccg gccgcccatc ttctgctgat
      601 ggcggccgcg cccgccgact tccgcgccgc ggcgcccgcc gaccagaaga tggtccgcgc
      661 tgacgggctc gagctgcgcc tcgagccgac cgaggacatc cttgccgggc tcaccgccca
      721 gcgcgccccc ggccagacga tcgtcggctt cgccgccgag cacggcggcg acgcggtcgg
      781 ccgcgcccgc gagaagcgcg cccgcaaggg agtcgacatg atcgtcctca acgacgtctc
      841 caatcccgag atcggcttcg agtcgacccg caacgcggtc accctggtcg acgccgaggg
      901 cgagaccgag gttgcgatcg ccagcaagga cgcaattgcc gacgcgattc tggaccattg
      961 cgagcgcttg caggagaaag cgggtatagc cgagtagatg agcgggctat actggctgca
     1021 agcttttcat gcctagaagt acggagaagt gggcctggcc cacttttttt acgcccggag
     1081 ctggtttgac cgatctacag cagagcattc aagagcagct tgccgatctc gattcggagc
     1141 ttgacgtcgt tgccctggag cgcccgggga acgagacgct gcggatcttc atcgaccacc
     1201 cgggcggcgt tgacctggct ctctgcgaac gggtgaccaa gcacctcaac cacctgctcg
     1261 tcgattactc gctggaggtt tcctcgccgg gcccgaagta ccgtcgttcc agcacctctt
     1321 ccgataccaa ggagtcgccc cagtgaccaa agagatcgtc gacgcggtca aagcgctcga
     1381 gcaggagaag ggaatctccg ccgacaagtt gatggacgcc ctcgaggacg ccctgctgtc
     1441 cgcgtacaag aagaccccgg gcgcggccaa atatgcccgt gtcgacctcg atcacgaaac
     1501 cggcgacttc cgcgtctacg agctgatcct gccccctgag ctggaggaga aactgctcgc
     1561 cgaagcggcc gagcagcagg acgagcccga agtcgacccg gagaccggcg aactgaaaga
     1621 gccggaggac cccgagctcg acccggagat gctcgctcct tacgagtcgc agatcacgac
     1681 caaagacgtc acccctgaag acttcggccg gatcgccgcg cagaccgcca agcaggtgat
     1741 cctgcagcgg attcgcgagg ccgagcggca gatgatgtac gacgagtacc aggatcgggt
     1801 cggcgagctg atcaccggga tcatccagca gtccgacaag cgctacacgc tggtccagtt
     1861 gcgtgagcgg gtcgaggcgc tgctgccgaa gtccgagcag gtctacaacg agcgctacga
     1921 ccacgggatg cgggtcaagg ccgtgatcac cgacgtctcc gactcgacca aaggcccggc
     1981 gatcgtcgtc tcgcggcgta gtccggagct gatcaaaagc ctgttcgagc tcgaggtgcc
     2041 ggagatcgcc gacaagctgg tcgaaatcgt cggcgtcgcc cgcgagcccg gctaccgctc
     2101 gaagatcgcc gtcgtccccc acgccgacgg cgtcgacccg gtcggcgcct gcgtcggccc
     2161 gcgtggctcg cgggtgcgga tggtcgtctc cgagctccgc ggcgagaaga tcgacatcat
     2221 cccctacaac gaggagcccg cccgcttcgt cgccaaagcg ctttcgccgg cccgc
//
LOCUS       DASRIG010000049         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3414975, whole genome shotgun sequence.
ACCESSION   DASRIG010000049 DASRIG010000000
VERSION     DASRIG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3414975"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFB52_01315"
     CDS             <1..467
                     /locus_tag="VFB52_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HZO05001.1"
                     /translation="VMKTATTHLTFDTPNRRELIRITEDVQACVDEAGIAEGMVLASA
                     MHITAGIWVNDDEPGLHEDVIEWLDKVAPPSWMPAANGVAEDLLPSGGDYRHHRGGED
                     NGDAHLKNLLVHHQAIVPVTAGRLDLGPWQQVFYCEFDGGRPKRVVIKAMGE"
     gene            472..1053
                     /locus_tag="VFB52_01320"
     CDS             472..1053
                     /locus_tag="VFB52_01320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZO05002.1"
                     /translation="MDLTVRLEGELVVLEPLADSHGDELFEAAQAPETFQWLAHIGRD
                     RQYFDYWLELTLTNAAEGREGPFAIRRQADGRLVGSSRYMAVRPADRALEIGWTWFNP
                     SAWRTGINLETKLLMLGRAFDDLECVRVEFKTDARNERSRNAIAALPAQFEGVLRNHM
                     IVPDVGQRDSAYYSVIDREWPEVRANLERRLGR"
     gene            complement(1106..1990)
                     /locus_tag="VFB52_01325"
     CDS             complement(1106..1990)
                     /locus_tag="VFB52_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05003.1"
                     /translation="MSLTFASFVTEFWENQLVEHERQHVFLVLVGFIGSFAFIRMSTR
                     LMRSPKVPWWPGSVSTGGVHVHHLVFGIIAMMIAGTLAFAAAGHSPTMEICALLFGIG
                     MGLTIDEFALWVYLDDVYWAEEGRSSIDATVIAAGAIGLVVLGVNPFDLGDGSEGVPA
                     VLASAALLGLCVGICFFKGRRLHGIIGFFLFPIALYGACRIGKPDSAWARRRYGKRNP
                     KKQLKAEERFHPGRRTDRFKEAFRDIIGGKPSEGLEAVKDEALAATREAGEATREAGE
                     EVRQKAEKLTHPEDRRKD"
     gene            complement(1987..>2718)
                     /locus_tag="VFB52_01330"
     CDS             complement(1987..>2718)
                     /locus_tag="VFB52_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05004.1"
                     /translation="IGRVYWSWAPTLLLLAAIVFLPLGLVGAVATEIDLDSIDVDSAA
                     ELAVLIGALGAITATSLLGEVFYSGAIAISLTHPAHDHPPKLREIARRVNYRRLIVID
                     IVYVALVLVGLAAFLIPGLLIFIYLGLAGPVVEIEERTIRQAFRRSLSLVRGNFWLVF
                     FVLAPVEIAGDVIGEYLGHLIHEALYDHFIGTWLAETVSSSVLSPIYAVAVVLLTLDL
                     IEQKDGTAPALKHQPAPVAKPAVSA"
BASE COUNT          468 a          868 c          971 g          411 t
ORIGIN      
        1 gagtgatgaa aaccgccacc acccacctca ccttcgacac ccccaaccgg cgcgaactga
       61 ttcggatcac cgaagacgtg caggcttgcg tcgacgaggc cgggatcgcg gagggcatgg
      121 tgctggcatc ggcgatgcac atcaccgccg ggatctgggt caatgacgac gagccagggc
      181 tgcacgagga cgtgatcgag tggctcgaca aagtggcgcc gccgagctgg atgccggcgg
      241 ccaacggggt cgccgaggat ctgctgccca gcggcggcga ctaccggcac caccgcggcg
      301 gtgaggacaa cggggacgcg cacctgaaga acctgctggt ccaccaccag gcgatcgtgc
      361 cggtgacggc gggacggctg gacctggggc cgtggcagca ggtcttctac tgcgagttcg
      421 acggcggccg gccgaagcgg gtcgtgatca aggcgatggg ggagtagcgg ggtggacctg
      481 acggttcggc tggagggcga gctggttgtg ttggagccgc tcgcggacag tcacggcgac
      541 gaactgttcg aggccgcgca ggcgcccgag acctttcagt ggcttgcgca catcggtcgc
      601 gaccgccagt acttcgacta ctggctggag ctgactctga ccaacgccgc cgagggccgg
      661 gaggggccgt tcgcgatccg acggcaggcc gacggcaggc tggtcggcag cagccgctac
      721 atggcggtgc gcccggccga ccgggcgctg gagatcggct ggacctggtt caacccgagc
      781 gcctggcgga ccgggatcaa cctggagacc aaactgctga tgctggggcg cgcgttcgac
      841 gacctggagt gcgtgcgggt cgagttcaag accgacgccc gcaacgaacg ctcgcgcaac
      901 gcgatcgcgg cgctgccggc ccagttcgag ggcgtcctgc gcaaccacat gatcgttccc
      961 gacgtcggcc agcgcgacag cgcctattac tcggtgatcg accgcgagtg gccagaagtg
     1021 cgcgccaacc tcgaacgacg cctcggtcgc tgacgcgctt gtccttttga gctcgctata
     1081 gcgtgtacaa aaggacgagc gcggctcagt ccttgcgccg gtcttcgggg tgggtcagtt
     1141 tttcggcttt ttggcgcact tcttccccgg cttcgcgggt cgcttccccg gcttcgcggg
     1201 tcgccgccag ggcctcgtct ttgaccgctt caagtccctc gctcggcttg ccgccgatga
     1261 tgtcgcggaa ggcttccttg aagcgatcgg tccgccgtcc cgggtggaag cgctcttccg
     1321 ccttcagctg cttcttcggg ttgcgcttgc cgtagcgccg ccgcgcccag gccgaatccg
     1381 gcttgccgat ccggcaggcg ccgtagaggg cgatcgggaa gaggaagaag ccaatgatcc
     1441 cgtgcaggcg ccggcccttg aagaagcaga tgccgacgca gagcccgagg agcgccgccg
     1501 aggcgaggac ggcaggcacc ccttcggagc cgtcgccgag gtcgaacggg ttgaccccga
     1561 ggacgaccag gccgatcgcc ccggccgcga tcacggtcgc gtcgatcgag ctgcgcccct
     1621 cctcggccca gtagacgtcg tcgagataga cccagagcgc gaactcgtcg atcgtcagcc
     1681 ccatcccgat cccgaacagc agcgcgcaga tctccatcgt cgggctgtgg ccggcggcgg
     1741 cgaaggccag ggtgccggcg atcatcatcg cgatgatccc gaagaccagg tggtggacgt
     1801 gaacgccgcc ggtgctgaca ctgccgggcc accagggcac cttcgggctg cgcatcagcc
     1861 gggtgctcat gcggatgaag gcaaaggagc cgatgaaccc gacgagcacg aggaagacgt
     1921 gctggcgctc gtgttcgacc agctggttct cccagaattc ggtgacgaac gaggcgaagg
     1981 tgaggctcac gcgctcaccg ccggtttcgc gaccggggcg ggttggtgct tcagcgccgg
     2041 ggccgtgccg tccttctgct cgatcaggtc gagggtgagc aggacgacgg cgactgcgta
     2101 gatcggcgag agcacgctgc tcgagacggt ctcggcaagc caggtgccga tgaagtggtc
     2161 atatagcgct tcgtggatca ggtggccgag gtactcgccg atcacgtcgc cggcgatctc
     2221 gaccggcgcc aggacgaaga agacgagcca gaagttcccg cgcaccaggc tgaggctgcg
     2281 gcggaacgcc tggcggatcg tgcgctcctc gatctcgacc accggcccag ccaatccgag
     2341 gtagatgaag atcagcaggc ccgggatcag gaacgcagcc agcccgacca ggaccagggc
     2401 gacgtagacg atgtcgatca cgatcaggcg cctgtagttg acccgccgcg cgatctcgcg
     2461 cagcttcggc gggtggtcgt gcgcgggatg ggtaaggctg atcgcgatcg cgccgctgta
     2521 gaagacctcg ccgagcaggc tggtcgcggt gatcgcgcca agcgctccga tcagtactgc
     2581 gagctcggcg gcgctgtcga cgtcgatcga gtcgaggtcg atttcggtgg cgaccgcgcc
     2641 gaccaggccg agtggcagga agacgatcgc cgcgagcagc agcagggtgg gggcccagga
     2701 ccagtagacg cggccgat
//
LOCUS       DASRIG010000050         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_546027, whole genome shotgun sequence.
ACCESSION   DASRIG010000050 DASRIG010000000
VERSION     DASRIG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546027"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFB52_01335"
     CDS             <1..384
                     /locus_tag="VFB52_01335"
                     /inference="COORDINATES: protein motif:HMM:NF021483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HZO05005.1"
                     /translation="RGFESHPLRLHRIYKISFASIYPHYVAKAEKKGRTQAEVDEIIR
                     WLTGYDQKGLEAQLENETNLETFFAKAPRPNPSRNLIKGVICGVRVEEIEETTMREAR
                     YLDKLIDELAKGKAMEKILRTPSVA"
     gene            429..1310
                     /locus_tag="VFB52_01340"
     CDS             429..1310
                     /locus_tag="VFB52_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO05006.1"
                     /translation="MEREDLVFRSQGEDCAAWLYPATAGSAPTPLIVMAHGLSGTRRD
                     RLGPFAERFAAAGVAALVFDHRGFGDSDGTPDLFHPRRQLDDWRAAIAFARSLPGVDA
                     ERIATFGSSMGGGNALAAAAEDGRVAAAISQVPFLDLGQAHRSRPLVRLRMVGAAVFG
                     RHLPAVGQPYDAAFINAPDSEDGWRHVVAIGEDSRWRNRVSSRWLLGGGFRTSQHAAG
                     LHCPWLVCVGEADQVAHPGAAIAAARRAPQGELRAYPGVDHFDIYDGPEHEALVADQL
                     AFLRRHLSISSGVKAVP"
     gene            1321..2157
                     /locus_tag="VFB52_01345"
     CDS             1321..2157
                     /locus_tag="VFB52_01345"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZO05007.1"
                     /translation="MEWWEAILLGAVEGFTEFLPISSTGHLTILESALGYSTDGADIT
                     AFTAIIQSGAVLATLLFLRRDIVRLLGAWFRGLADRDQRTGRDWVFAWAVILGSLPII
                     VVGLAFKDQIETTLRSLWFVGTALILFSGVMYYADSRATQRRHEEDVTWRDTLIIGIT
                     QCIALIPGISRSGATMSAGLLREIDRVAVTRLSFFLSIPALTGAAILQSFTKADEISN
                     GVGWGPTLLATAVSFVVAYFAIFWLLRYIARHNYAIFIVYRVVLGTLVLLLVATGVLS
                     AT"
     gene            complement(2154..2585)
                     /locus_tag="VFB52_01350"
     CDS             complement(2154..2585)
                     /locus_tag="VFB52_01350"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZO05008.1"
                     /translation="MALALDQARQAEAHGDVPIGAAITGADGELLAAAGNERELRRDP
                     TAHAEILAIRAAAEALGGWRLPGTTLYVTLEPCAMCAGAIVLARIPTVVIGAPDPKAG
                     AAGSVLDVLAEPALNHRPEVIHGIREPESAALLRKFFAARR"
     gene            2732..4108
                     /locus_tag="VFB52_01355"
     CDS             2732..4108
                     /locus_tag="VFB52_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO05009.1"
                     /translation="MKRFFCIPAVLAALALSLFALPASAEIGLGQARGFVPHELIVKV
                     EGQPAKTVELRDGLGVGVAAQALGDKARVEYAAPNYIATASATPVPFVLPNDPGNIGG
                     PAEAASAPGDWTLKQWNFLPYEAAAGSKLTTSPGGIDVVGAWENLAAAGRPGGQGVTV
                     AVLDTGIAYRNYETTLNQGLSFLRSPDFTAAQFVKGYDFVENDRIPLDLNGHGTHVAG
                     TIAEKTDNGIGLTGIAYRAKLMPVRVLDSQGRGDAAAIARGIRFAIANRAQVINMSFN
                     FGCGKQVPGVNEALREAANKGIVVVASAGNLGAETCVSPPATGPRVIGVGGSTEGGCL
                     GSYSLAGRGVDLLAPGGGTPIAGCPSISARPIYQVTLKPTSSHEFGVPSNYVGTSMAA
                     AHVSGAAALVLASGLVKNKAGTKGRVDWVRRRLTGTARSLGLTSLQQGHGLIDIAAAT
                     SMRAARHR"
     gene            complement(4130..>4594)
                     /locus_tag="VFB52_01360"
     CDS             complement(4130..>4594)
                     /locus_tag="VFB52_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO05010.1"
                     /translation="DEEAALALFNRIVVGTDGSETAAAAVGQAIELAKLSGAELSIVS
                     AYAPVHKKRLEGEVSGVPADVQHQIGPREDVNLVLDAAAAQARSAGVEVQTHPVEADP
                     ADAILNVAEQINADLIVVGNKGMTGARRYLLGSVPNNVSHHAPCSVIIIRTT"
BASE COUNT          646 a         1660 c         1584 g          704 t
ORIGIN      
        1 cgtgggttcg aatcccaccc tctccgtttg caccggatct acaagatcag cttcgccagc
       61 atctacccgc actacgtcgc caaggcggag aaaaaggggc gcacccaggc ggaggtcgac
      121 gagatcatcc gctggctgac cggatacgac cagaagggcc tcgaagccca actcgagaac
      181 gagaccaacc tcgagacgtt cttcgccaag gccccgcggc cgaacccgtc gcggaacctg
      241 atcaagggcg tgatctgcgg cgtccgggtc gaggagatcg aggagacgac gatgcgggaa
      301 gcccgctacc tggacaaact catcgacgag ctggcgaagg ggaaagcgat ggagaagatc
      361 ctgcggaccc cgagcgtggc ctagccgcgt tcatctcacg ttctggcggc cggccgcgtt
      421 ctatcggcat ggagcgtgag gatctggtct tccggtcgca gggtgaggat tgcgccgcct
      481 ggctataccc ggccacggcc gggtccgcgc ccacgcctct gatcgtgatg gcgcacgggc
      541 tgtcggggac gcggcgggat cggcttgggc cgtttgcgga gcggttcgcg gcggccgggg
      601 tcgcggcgct ggtcttcgat caccgggggt tcggcgacag cgacgggacg ccggacctct
      661 tccacccgcg gcgccaactc gatgactggc gggcggcgat cgcgtttgcg cgctcgctgc
      721 cgggggtcga cgccgagcgg atcgcgacct tcgggtcctc gatgggcggc ggaaacgcgc
      781 tcgcggcggc cgctgaagac gggcgggtcg cggcggcgat cagccaggtg cccttcctcg
      841 acctcggcca ggcgcaccgc tcgcggccgc tcgtgcgcct gcgcatggtc ggcgctgcgg
      901 tgttcggtcg ccacctgccg gcggtcgggc agccctacga cgccgccttc atcaacgccc
      961 ccgacagcga ggacgggtgg cggcacgtgg tcgccatcgg tgaggactcg cgctggcgca
     1021 accgggtctc ctcgcgctgg ctgctcggcg gcggcttccg caccagccag cacgccgccg
     1081 ggctgcactg cccctggctg gtctgcgtcg gcgaggccga ccaggtcgca caccccggcg
     1141 ccgcgatcgc cgctgcgcgc cgcgctccgc agggcgagct gcgcgcctac ccgggcgtcg
     1201 atcacttcga catctacgac ggtcccgagc atgaggcgct ggtcgccgac cagctcgcct
     1261 tcctgcggcg ccacctctcg atctcatctg gcgttaaggc ggtcccatag tctctgccgc
     1321 ttggaatggt gggaagccat actcctcggc gcagtcgagg gctttaccga gttcctgccg
     1381 atctcgagca ccgggcacct gacgatcctc gagagcgccc tcggctactc gaccgacggc
     1441 gccgacatca ccgcgttcac ggcgatcatc cagagcggtg cggtgctggc gacgctgctc
     1501 ttcctgcgtc gcgacatcgt gcggctgctc ggcgcctggt tccgtggcct ggccgaccgc
     1561 gatcagcgca ccgggcgcga ctgggtcttc gcctgggcgg tgatcctcgg ctcactgccg
     1621 atcatcgtcg tcgggctcgc cttcaaggac cagatcgaga cgaccctgcg cagcctctgg
     1681 ttcgtcggca cggcgctgat cctgttcagc ggggtcatgt actacgccga cagccgcgcg
     1741 acccagcgcc gccacgagga ggacgtgacc tggcgcgaca cgctgatcat cgggatcacc
     1801 cagtgcatcg cgctgatccc ggggatctcg cgctcggggg cgacgatgtc ggccggcctg
     1861 ctgcgcgaaa tcgaccgcgt cgcggtcacc aggctctcct tcttcctctc gatcccggcc
     1921 ctgaccggcg ccgcgatcct gcagagcttc accaaggccg acgagatctc caacggcgtc
     1981 ggctgggggc cgaccctgct ggcgaccgcg gtcagcttcg tcgtcgccta cttcgcgatc
     2041 ttttggctgc tccgctacat cgcccgccac aactacgcga tcttcatcgt ctaccgcgtc
     2101 gtgctcggca ccctcgtgct gctcctggtc gccaccggcg tgctctcggc gacttagcgc
     2161 cgcgcggcga agaacttgcg gaggagggcg gcggactcgg gctcgcggat gccgtggatc
     2221 acctcggggc ggtggttcag ggccggctcg gcgagcacgt cgaggacgct gccggcggcg
     2281 cccgccttcg ggtcgggggc gccgatcacg acggtgggga tacgggcgag gacgatcgcg
     2341 ccggcgcaca tcgcgcacgg ctccagggtc acgtagaggg tcgtcccggg caggcgccag
     2401 ccgccgagcg cctcggcggc ggcccggatc gcgaggatct cggcgtgggc ggtcgggtcg
     2461 cggcgcagct cgcgctcgtt gccggcggcg gccagcagct cgccgtcggc gccggtgatc
     2521 gcggccccga tcggcacgtc gccgtgcgcc tcggcctggc gcgcctggtc cagggccagc
     2581 gccattcccg cttcatggcc atctattcgg tcgtgccgat tcatccaatc cgggagtatg
     2641 tccctacaac ttcgccgctg tcggggtgtt agccagtaga ggctcgcccg gccactcgat
     2701 tcggcgagca caggacacga tcttttttta gatgaagcga tttttctgca tccctgccgt
     2761 tttggcagca ctggcgctgt cgctcttcgc cctgcccgcg agcgccgaga tcggcctcgg
     2821 gcaggcgcgc gggttcgtgc cccacgagct gatcgtcaag gtcgaggggc agccggcgaa
     2881 gacggtcgag ctgcgcgacg ggctcggcgt cggcgtggcc gcgcaggcgc tgggcgacaa
     2941 ggcccgggtc gagtacgccg ccccgaacta catcgccacc gcctcggcga ccccggtgcc
     3001 gttcgtgctc cccaacgacc ccggcaacat cggcggcccc gccgaagccg ccagcgcacc
     3061 cggcgactgg acgctgaagc agtggaactt cctgccctac gaggcggcgg cgggcagcaa
     3121 gctgacgacc tcgcccggcg ggatcgacgt ggtcggcgcc tgggaaaacc tcgccgcggc
     3181 gggccgcccc ggcggccagg gggtgacggt cgcggtgctc gacaccggga tcgcctaccg
     3241 caactacgaa acgacgctga accagggcct ctccttcctg cgcagccccg acttcaccgc
     3301 cgcccagttc gtcaagggct acgacttcgt cgagaacgac cgcatccccc tcgacctcaa
     3361 cgggcacggc acccacgtcg ccggcacgat cgccgagaag accgacaacg ggatcggcct
     3421 gaccgggatc gcctaccggg cgaagctgat gccggtgcgg gtgctcgact cgcagggccg
     3481 cggcgacgcg gcggcgatcg ccagggggat ccgcttcgcg atcgccaatc gcgcccaggt
     3541 gatcaacatg agcttcaact tcggctgcgg caagcaggtc ccgggcgtca acgaagcgct
     3601 gcgcgaagcg gcgaacaaag ggatcgtcgt cgtcgcctcc gccggcaacc tcggcgccga
     3661 gacctgcgtc tcgccgccgg ccaccgggcc gcgcgtgatc ggcgtcggcg gcagcaccga
     3721 gggcggctgc ctcggcagct actcgctggc cggccgcggc gtcgacctgc tcgcgccggg
     3781 cggcggcacc ccgatcgccg gctgcccctc gatctcggcc cggccgatct accaggtgac
     3841 gctgaaaccg acctcgagcc acgagttcgg cgtgcccagc aactacgtcg gcacctcgat
     3901 ggccgccgcc cacgtctccg gcgccgcggc gctggtcctg gccagcggcc tggtcaagaa
     3961 caaggcgggc acgaaggggc gggtcgactg ggtacgccgc cgcctcaccg gcaccgcgcg
     4021 cagcctcggc ctcacctcac tgcagcaggg ccacggcctg atcgacatcg ccgccgcgac
     4081 gtcgatgcgc gccgcccgcc accgctagcc cccaacccgc gtcctggggc taggtcgttc
     4141 tgatgatgat gacgctgcaa ggagcgtggt gggaaacgtt gttcggcacg ctgccgagca
     4201 ggtaacggcg ggcgccggtc atccccttgt tgccgaccac gatcaggtcg gcgttgatct
     4261 gttcggcgac gttgaggatc gcgtcggccg ggtcggcctc gaccgggtgg gtctgtacct
     4321 cgacccccgc gctgcgcgcc tgcgcggcgg ccgcgtcgag gaccaggttg acgtcctcgc
     4381 gggggccgat ctgatgctgg acgtccgcgg ggacgccgct cacctcgccc tccagccgtt
     4441 ttttgtgcac cggcgcgtag gcgctgacga tgctgagctc ggcacccgag agcttcgcca
     4501 gctcgatcgc ctgcccgacc gctgccgcgg ccgtctcgga gccgtcggtc cccaccacga
     4561 tgcgattgaa caacgccaaa gccgcctcct cgtc
//
LOCUS       DASRIG010000051         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1335381, whole genome shotgun sequence.
ACCESSION   DASRIG010000051 DASRIG010000000
VERSION     DASRIG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335381"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFB52_01365"
     CDS             <1..1065
                     /locus_tag="VFB52_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05011.1"
                     /translation="ERERLTEIEDVLGGSANDTLLGASVTPNRLDGGPGNDRIVGVGP
                     GDHAFGGPGSDDCRGQLTEQVSCGPSGSRRHTAVDVYRSIGNTTSLVISGSRRANHVV
                     VNRNDAGYVVASHTPGVGVRLGDRHSDSCRRAERQQTILCTGTVTSILASLGPGPDTL
                     EIAPEVPASVSAVIDGGGGADNLRGGGGNDVIYAGDDRAADTLAGAGGDDVLFGVNIF
                     HPRLSSGAAQMIGGPGNDLMVGGQPCEGDFFHGGPGDRDSASFARVRNDGIAVVAAIG
                     GAVTDPDLPGCQGGRIDPSTEKIEGSPGPDRLLGNVGPNWLLGRGGDDFLDGRGGYDR
                     CAGGAGRDSARGCERTGSVP"
     gene            1641..1838
                     /locus_tag="VFB52_01370"
     CDS             1641..1838
                     /locus_tag="VFB52_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05012.1"
                     /translation="MPTYLRFGDDHSVAVNETYEEVKKLIVTAAAQPVPMFEVTRVDG
                     ARLFVNADELWTVSEGRQKPE"
     gene            1935..2450
                     /locus_tag="VFB52_01375"
     CDS             1935..2450
                     /locus_tag="VFB52_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZO05013.1"
                     /translation="MKVLVTHSSKRGGTAGIAEAIADELQRRGLEAECVDVGEVDDLS
                     DYGAVVLGSGVYAKRWRGEAKRFLRGHRKELRSMPFWIFSSGPVGDPAKDDPKWSEPP
                     RVIARAEKLGVREHAVFGGCVPAEPHGFVEKAMVENTPPEFRDRRDWDAIRTWAAAIA
                     AELQPASTAAR"
     gene            complement(2434..2841)
                     /locus_tag="VFB52_01380"
     CDS             complement(2434..2841)
                     /locus_tag="VFB52_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZO05014.1"
                     /translation="MDASAGIDAPTGPEPLNKEGFAAFAARTEEQAPVVMLNLLRFKP
                     DGGRERYGEYGAAVAPLLEAAGGRIVFAGDPATVLLGERSWDMVLLVEYPTRSAFLEM
                     IGSEAYQTIAHLRTEALVAGELHPLDPGAIELP"
     gene            complement(3000..3275)
                     /locus_tag="VFB52_01385"
     CDS             complement(3000..3275)
                     /locus_tag="VFB52_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HZO05015.1"
                     /translation="MKFLFAPVGIGAGIVAGMLGKKAFERLWAVVDDEDPPEPDQRDA
                     PTLKLVAALAVEGAIFRVAKGLTDYGARRAFASFTGRWPGEDPARDA"
     gene            3373..3918
                     /locus_tag="VFB52_01390"
     CDS             3373..3918
                     /locus_tag="VFB52_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZO05016.1"
                     /translation="MHHHRHDPREIAALRQEFARSRGPRYGRRGRGGMRARRGDVRAA
                     ILALLAERPMHGYEMIKELEERTQGAWTPSAGSIYPTLQMLEDEGLVRGEESEGKRRF
                     ALTDAGQTEQTEKAGEQTPWDAVRADAAPEAINLKDSMGKLNHAIAQSFHAADEGQQQ
                     RVRELLDETRRKIYAILAEEG"
     gene            complement(4192..6231)
                     /locus_tag="VFB52_01395"
     CDS             complement(4192..6231)
                     /locus_tag="VFB52_01395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZO05017.1"
                     /translation="MSEEPTATQGNEDEKREPLLPGINAPADLHELDDAQLQQVSEEM
                     RTYIIDTIGEIGGHFGANLGTCELAVALHSLLDSPRDKVLWDVGHQAYPHKILTGRRE
                     ELPTIRQYEGLAPFCSIAESEHDIMGAGHASTSIGYAVGLKEAMRKGIGPDGKVAAVI
                     GDGALTGGVAYEALHAAGGLQTPIVIVLNDNGMSISPNVGALSRYFNRIRLNPRLYHA
                     REGVEDRLTKLPLGLGRRIERLGPEIKSAIKAYWAPGLFFEELDLAYMGVIDGHDVHA
                     LREALTEAFEAERPVVVHIHTVKGKGFEPAEAGGLQTMEEWHAAKPNSIVNGKPAAQK
                     AKTVAVTETDSPEGIERLEKPPEVGSPPQYTKVFAEAMVAEAKRDERVIGITAAMAGG
                     TGLQQLADEVPEQYYDVGIAEQNAVLLASGLALQGAKPVCAIYSTFLQRAYDQIVHDV
                     CLQDLDVTFAMDRAGLVGDDGPTHHGAFDVSYFRPIPNVVVMAPRDEAALVHMLHTAI
                     AHDGPAALRYPRGAGEGVELPVKPKIIPIGVGEILAEGERVALLGYGYGVSVALEAAE
                     ILAGHGLKVTVADARFAKPLDGELAERLAREHELLVTIEENVLPGGFGAAVLEHLEDS
                     IENPGERARVLRVGLPDRYVTHGKPALLRGEVGLTGQSVADRVLASTPSAQPVLG"
     gene            complement(6228..>6926)
                     /locus_tag="VFB52_01400"
     CDS             complement(6228..>6926)
                     /locus_tag="VFB52_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZO05018.1"
                     /translation="CGGADRAAAPALAAIELLHTYTLVHDDLPAMDDDDLRRGQPTSH
                     VKYGEDVAILAGDGLFAEAMRLFCEQPGEPARVLAALRELAAATGVDGMVGGQYVDVI
                     EAADDAEGLRALHGLKTGRLIAASVGVVLILEGVGEHETMPYRRFADEIGVLFQIVDD
                     ILDVTASDEQLGKPHGSDERHGKLTYVSLFGLEQARELAAESHAKATAALAEANGATE
                     DLRRVADYIFTRNE"
BASE COUNT         1043 a         2464 c         2377 g         1042 t
ORIGIN      
        1 gagcgcgagc gcctcaccga gatcgaggac gtgctcggcg gctccgccaa cgacaccctg
       61 ctcggcgcca gcgtcacccc gaaccggctc gacggcgggc cgggcaacga ccgcatcgtc
      121 ggtgtcggtc cgggcgacca cgccttcggc ggtcccggca gcgacgactg ccgcggccag
      181 ctcaccgagc aggtctcctg cggacccagc ggcagccggc ggcacaccgc ggtcgacgtc
      241 taccgcagca tcggcaacac caccagcctg gtgatcagcg gcagtcgccg cgccaaccac
      301 gtggtcgtca accgcaacga cgccggctac gtggtcgcca gccacacgcc cggggtcggc
      361 gtgcgcctcg gcgatcgcca ctcggacagc tgccgccggg ctgagcgaca gcagacgatt
      421 ctctgcaccg gcaccgtcac ctcgatcctc gcctcgctcg ggcccgggcc ggacacgctg
      481 gagatcgccc ccgaggttcc cgcttcggtc tccgccgtga tcgacggcgg cggcggggcc
      541 gacaacctgc gcggcggcgg cggcaacgac gtgatctacg ccggtgacga ccgcgcagcc
      601 gacacgctcg cgggcgcggg cggcgacgac gtcctcttcg gcgtcaacat cttccacccg
      661 cgcctcagca gcggcgccgc gcagatgatc ggtgggcccg gcaacgacct gatggtcggt
      721 ggccagccct gcgagggcga cttcttccac ggcggccccg gtgaccgcga cagcgcctcc
      781 ttcgcccgcg tgcgcaacga cgggatcgcg gtggtcgcgg cgatcggcgg cgcggtcacc
      841 gatccggacc tgccgggctg ccaaggcggt cgcatcgacc ccagcaccga gaagatcgag
      901 ggctcacccg gacccgaccg cctgcttggc aacgtcggtc cgaactggct gctcgggcgc
      961 ggcggcgacg acttcctcga cggccgcggc ggctacgacc gctgcgccgg cggcgccggc
     1021 cgagacagcg cccgcggctg cgagcgcacc ggctccgttc cctagtccgg ttgtgcggga
     1081 gggctcgcct ggttacactg cccggcaatg ctcaacctcc gcggacaagc cgttgttgcg
     1141 aagctttccg ttacggagaa aatcggcgcc gggctcgtta gctcggttgt cgtctgagca
     1201 ctcaagaacg cccgccgacc agcagcagct acatcgaaag ggacgcggcg gaggcgttag
     1261 aagccgttcg atccccttcc gatcgctccc catctaagcg ctggcaggaa gccgtgcgcg
     1321 ccgcggccac ccgccaggcc acacagggca gccaccgcca ccggccgcgc tgaggtccca
     1381 cgtgtcctgg gcaggtcttg ccctggctcc tagactggat agatgggagc cctgccgatc
     1441 gtcctgatca tcctcgcgat gaagatcccg atcttcggcc tgctcgcctt cgtctggtgg
     1501 gccgcgcgct cctacgacga acccggcgcc gagaaggaac cgcgcaagca acggccccgc
     1561 ccgcgccgcc ccggccccga gccccatcgt ggaccccgcc gtcgcggacc gcatggcggc
     1621 gaagcggtta gggtcccggc atgcccacct accttcgctt cggcgacgac cactcggttg
     1681 cggtcaacga gacctacgag gaagtcaaga aactgatcgt cacagcggcg gcgcagccgg
     1741 tgccgatgtt cgaggtcacc cgggtcgacg gcgcccgcct gttcgtcaac gccgacgagc
     1801 tctggaccgt ctccgagggc cggcagaagc ccgagtagcg gcgcggccct cccgggccgc
     1861 tgcgggagtt ccggcgccgg gtctgcggca ggacacggat ggcggcggcg cgacgcgctg
     1921 cgagactggc ggcgatgaag gtactcgtca cccacagctc caaacgcggt ggcaccgcgg
     1981 gaattgccga ggcgatcgcc gacgagctcc agcggcgcgg cctcgaggcc gagtgcgtcg
     2041 acgtcggcga ggtcgacgac ctcagcgact acggcgcagt ggtgctcggc agcggcgtct
     2101 acgcgaaacg gtggcgcggc gaggccaagc ggttcctgcg cgggcatcgc aaggagctca
     2161 gatcgatgcc gttctggatc ttcagctccg gcccggtcgg cgaccccgcc aaggacgacc
     2221 cgaaatggtc ggagcccccg cgcgtgatcg ctcgcgccga aaagctcggg gttcgcgaac
     2281 acgccgtctt cggcggctgc gtgccggccg agccgcacgg cttcgttgag aaggcgatgg
     2341 tcgagaacac gccgcccgag ttccgcgatc gccgcgactg ggacgcgatc cgcacctggg
     2401 cggcagcgat cgccgccgag ctgcaaccag cgtctacggc agctcgatag cgcccgggtc
     2461 gagcgggtgc agctcgccgg cgaccagcgc ctcggtgcgc aggtgcgcga tcgtctggta
     2521 ggcctcggag ccgatcatct cgaggaaggc gctgcgggtc gggtattcga ccagcaggac
     2581 catgtcccag gagcgctcgc cgagcaggac cgtggcgggg tcgcctgcga agacgatgcg
     2641 ccccccggcc gcctccagca gcggcgccac cgcggcgccg tactcgccgt agcgctcgcg
     2701 gccgccgtcg ggcttgaagc gcagcaggtt gagcatcacg accggcgcct gctcctccgt
     2761 gcgcgccgcg aaggcggcaa acccctcctt gttcagcggc tcgggccccg tcggggcgtc
     2821 aatccctgca gaagcgtcca tctctcccct ccctcaaacc cgtttcagaa aaattcacca
     2881 aaccaagttt atggccgcaa gggcttgggt acctcactat ttctcccccg cagtgaaccc
     2941 ggaagcgccc ctcccctgcg aggggcgttt tcgcgttcta gagcggtttg cggcgccctt
     3001 taggcgtcgc gagcggggtc ctcgcccggc cagcggccgg tgaatgaggc aaacgcccgg
     3061 cgggcaccgt agtcggtcag ccctttggcg acgcggaaga tcgctccctc gacggcgagg
     3121 gcggcgacca gtttcagggt cggcgcgtca cgctggtcgg gctcgggcgg atcctcgtcg
     3181 tcgaccacgg cccagagccg ttcgaacgcc ttcttgccga gcatcccggc gacgatgccg
     3241 gcgccgatcc caaccggtgc gaagaggaac ttcatgcggg caggctaccc ggcagaggca
     3301 cgcagcatgt gcgagcccgt caacgcgagt tgttacgata tatcgcgtta gttaccgata
     3361 gaaaggtttt cgatgcacca ccaccgacac gacccaagag agatcgcggc cctgcgccag
     3421 gagttcgcgc gcagccgcgg gccccgttac ggccggcgcg ggcgcggcgg catgcgggcg
     3481 cggcgcggcg acgtgcgtgc ggcgatcctc gccctgctcg ccgagcggcc gatgcacggc
     3541 tacgagatga tcaaggagct ggaggagcgc acgcaggggg cctggacccc gagcgccggc
     3601 tcgatctacc ccaccctgca gatgctcgag gacgagggcc ttgtccgcgg cgaggagagc
     3661 gagggcaagc gccgcttcgc gctcaccgat gccggccaga ccgagcagac cgagaaagcc
     3721 ggggagcaga ccccctggga cgcggtccgc gccgacgccg cccccgaggc gatcaacctg
     3781 aaggactcga tgggcaagct gaaccacgcg atcgcccagt ccttccacgc cgccgacgag
     3841 ggccagcagc aacgggtccg cgagctgctc gacgagaccc gccgcaagat ctacgcgatc
     3901 ctcgccgagg agggctagtc gtttacggac gcgcctcgcg gccccggtcg cttttgacga
     3961 cgggccgcga gaagtccgtt ccccttacgg acccacacat atagagatgc gcgtctcctc
     4021 tccctgtggt gatttcccca gaagcgctgc tcttgcgagt ggcttcatcc accttcaccg
     4081 aggagacgtc cgcaggctat tcgattgcgt caaacggcgc aatccccaaa attgattacg
     4141 acgttgtaga gcggtggaaa tgcatccacc ggtcgtggca aaagggcctg gctagccgag
     4201 aactggctgt gcggagggcg tcgaggcgag cacccggtcg gccaccgact gaccggtcag
     4261 gccgacctcg ccgcgcaaca gcgccggctt gccgtgggtg acgtagcggt cgggcagacc
     4321 gacccgcagg acccgcgcgc gctcgcccgg attctcgatc gagtcctcga ggtgctccag
     4381 caccgcggcg ccgaaaccgc ccggcaggac gttctcctcg atcgtcacca acagctcgtg
     4441 ctcgcgcgcc agccgctcgg ccagctcgcc gtcgagcggc ttggcgaagc gggcgtcggc
     4501 gacggtcacc ttgagcccgt gtccggcgag gatctcggcc gcctccaggg caaccgagac
     4561 gccgtagccg tagccgagca gcgcgacccg ctcgccctcg gccaggatct cgcccacgcc
     4621 gatcggaatg attttcggct tgaccggcag ctccactccc tcccccgcgc cgcgcgggta
     4681 gcgcagcgcc gcggggccgt cgtgggcgat cgcggtgtgc agcatgtgga cgagggcagc
     4741 ctcgtcgcgc ggcgccatca cgaccacgtt cgggatcggc cggaagtagg agacgtcgaa
     4801 ggcgccgtgg tgagtggggc cgtcgtcgcc gaccaggccg gcgcggtcca tcgcgaaggt
     4861 gacgtcgagg tcctgcaggc agacgtcgtg aacgatctgg tcgtaggcgc gctggaggaa
     4921 ggtcgagtag atcgcacaga cgggcttggc gccctgcagg gccaggccgg aggcgagcag
     4981 aaccgcgttc tgctcggcga tcccgacgtc gtagtactgc tcgggcacct cgtcggcgag
     5041 ctgctgcagg ccggtccccc ccgccatcgc cgcggtgatc ccgatcacgc gctcgtcgcg
     5101 cttggcttcg gcgaccatcg cctcggcgaa gaccttcgtg tactggggcg gtgagcccac
     5161 ctccggcggc ttctccagcc gctcgatccc ctccggggag tcggtttcgg tgaccgccac
     5221 ggtcttcgcc ttctgcgccg ccggcttgcc gttgacgatc gagttcggct tcgccgcgtg
     5281 ccactcctcc atcgtctgca ggccgccggc ttcggccggc tcgaagccct tgcccttgac
     5341 cgtgtggatg tggacgacga ccgggcgttc ggcttcgaag gcctcggtca gcgcttcgcg
     5401 cagggcgtgc acgtcgtggc cgtcgatcac gcccatgtag gcgaggtcga gctcctcgaa
     5461 gaacagcccc ggcgcccagt aggccttgat cgccgacttg atctccggcc cgagccgctc
     5521 gatccgccgc ccgagcccga gcggcagctt ggtcagccgg tcctcgaccc cctcgcgggc
     5581 gtggtagagg cgcgggttga gccggatccg gttgaagtag cgggagagcg caccgacgtt
     5641 gggcgagatc gacatcccgt tgtcgttgag cacgatcacg atcggcgtct gtaggccgcc
     5701 ggccgcgtgc agcgcctcgt aggcgacacc gccggtcagg gcgccgtcgc cgatcaccgc
     5761 ggcgactttc ccgtcggggc cgattccctt gcgcatcgcc tccttcagcc cgaccgcgta
     5821 gccgatcgag gtcgaggcgt ggccggcacc catgatgtcg tgctcggact cggcgatcga
     5881 gcagaacggg gcgaggccct cgtactggcg gatcgtcggc agctcctcgc ggcggccggt
     5941 gaggatcttg tgcgggtaag cctggtggcc gacgtcccag aggaccttgt cgcgcgggga
     6001 gtcgagcagg ctgtgcagcg ccacggcgag ctcacaggtg ccgaggttgg caccaaaatg
     6061 accgccgatc tcgccgatcg tgtcgatgat gtaggtgcgc atctcctcgg agacctgctg
     6121 gagctgcgcg tcgtcgagct cgtgcaggtc ggcgggggcg ttgatccccg gcagcagcgg
     6181 ctcgcgtttt tcgtcttcgt tgccctgggt ggcggtgggc tcttcgctca ttcgttcctc
     6241 gtgaagatgt agtcggccac gcgtctcaga tcctcggtgg cgccgtttgc ttcagccagc
     6301 gcggctgtcg ccttcgcatg ggactcggcc gcgagctccc gcgcctgctc caacccgaag
     6361 aggctgacgt aggtgagttt tccatgtcgc tcgtcgcttc catgtggctt gccgagctgc
     6421 tcgtcgctgg cggtgacatc gaggatgtcg tcgacgatct ggaagagaac gccgatctcg
     6481 tccgcgaagc ggcgatacgg cattgtctca tgttcgccga cgccctcgag tatgagtacg
     6541 acaccgacgc tcgcggcgat cagtcggccg gtcttgaggc cgtgcagcgc tcgcaggccc
     6601 tcggcgtcgt cggccgcctc gatcacgtcg acgtactggc cgccgaccat gccgtcgacc
     6661 ccggtcgcgg cggccagctc acgcagcgcc gccaacaccc gcgccggctc gcccggctgc
     6721 tcgcagaaga gccgcatcgc ctcggcgaag aggccatcgc cggcgagaat cgcgacgtcc
     6781 tcgccgtatt tgacgtggct ggtcggttgg ccgcggcgca ggtcgtcgtc gtccatcgcc
     6841 ggcaggtcgt cgtgcaccag cgtgtaggtg tgcagcagtt cgatcgccgc cagcgccggc
     6901 gccgccgcgc gatcggcgcc gccgca
//
LOCUS       DASRIG010000052        10257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_282128, whole genome shotgun sequence.
ACCESSION   DASRIG010000052 DASRIG010000000
VERSION     DASRIG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282128"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VFB52_01405"
     CDS             complement(<1..1803)
                     /locus_tag="VFB52_01405"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZO05019.1"
                     /translation="MTSRKFIATLVVALLGLAALPSLASALTITVDDDRVQCPLADET
                     SLTAAVAVAANGDTVKICAGTYNVPGGPAPSSGLKIEKNINLAGAGSDKVFIQPTQGS
                     GSMAQATPNPRDEYGNVITVRRRLIELYDVSISGLTVRAGNVPVEAGIAMIDVVTGTI
                     SSVKVEGIVPTTGGNNPKPLPGTGAYAPEIESVPNPLSQYGNAVIVANTIEQTQNTTT
                     ITGSQFGSTTSGFNATGVLVDSRRLSGTEPVGNKSTVTANIVNTRINGAGGVQPIGQT
                     GVEAWGSGATVKLTKSKIAEVGKADGSAAAVEMHGVDLVNSVIGGSEADRNDLTANAY
                     GATNVAFDGSAAAASLKAGGNFWGTTTSAGVPLVGPNVDTEPTMPTAPAGPTLAPIAD
                     QLPIIEWEVKPVEGTVFAPGTSIPLAAVARDDFGVRTVEFRANGGTVSTAPTPPLLGE
                     RVYSGSWTPTAGYAGEVTISAVAVDANNLTSEVKVKVLVEGKPHFTAAPAITGFSGYA
                     SVGKPLTCSTGAAPAYPVPTYTYAWTVAGAPVGTDSPTYTPVAADQGKKVVCTVKATN
                     SFGSATSASGQVPVAKEPTFASPATITGGNTGVYA"
     gene            complement(1800..4346)
                     /locus_tag="VFB52_01410"
     CDS             complement(1800..4346)
                     /locus_tag="VFB52_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05020.1"
                     /translation="MKKVLVLAVSLAALVSVVLPVTALAVPPDPLQMGPYGVNRTYYD
                     AGTLQLTVPSSGVLPPGSGTCETGSGSTHEYGSSSKCPGQTFPQPLEGSITWPKSHPG
                     PYKVILFLHGRHSACKGTTGTDNGGQSFLTGLCPNPNVPVNPAGPSGPETVEDAGSVT
                     GFWPSWGGYNYISDLLASWGYVVISPSAGALVAFDGTSVMDAGAFARAEIIGNTLDIL
                     YSLNEGVPGPAPTSIQQANGQPGIGTQLAGKLDFSKVGIMGHSRGGEGVTEFIPFNRA
                     RPAPGRRYNLQAVFSLAPIDRNKQMPYGTNYATLLPACDGDVTTIAGANAFERGKYVA
                     PDDPYAKIQYYVEGANHNFYNTRWPSSDRGGSDIACGTAFGTGKARLTPEDQRKTGLA
                     TMPTFLRAYIGGETDLMPWLTGEAGLPASACATGPTHVPFCEDQVKQTYIAPAGERLD
                     ILRPGPGVRPTTASPIAPNEVGGMYSGTGFSTFQWCNPDPFANVMANPPATQTTTALV
                     ACEGPDRVLGTPPSTTTYTANQAIQSWGPQLTLAWNGPATLTTTLRGDARNATPYDNL
                     VFRAAFQSNDEALNPVGNYFDPRSATQDFWVVVTDREGQESAHKLTEFFSGGVEKALG
                     TTPNNSQHVVLNQFIAPLATFAADGVDVAALDRIEFRFGTAGVNPSGAIQFSDVAFQE
                     TPETAATPFAALKPLPSDPELPKLEALKTGAPRFATEPGYCVDKKKPTLTIKAVKSGK
                     KLVVTGKAGDKGCAAGAAKKASPGTVEQVQVSVFKEAGKKCRFLGADGRLMKKTPCTT
                     PLSVVASGKKHWKLRPPKRLPAGKYKVAAFAIDGAGNVSSVKFSRIEVGS"
     gene            complement(4569..4742)
                     /locus_tag="VFB52_01415"
     CDS             complement(4569..4742)
                     /locus_tag="VFB52_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05021.1"
                     /translation="MPPVFWIGLGIAAFIFLVGVISGARSRSASLGSGAAIGAFLGVM
                     LAFPLLAIGLATS"
     gene            4842..6413
                     /locus_tag="VFB52_01420"
     CDS             4842..6413
                     /locus_tag="VFB52_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05022.1"
                     /translation="MSETGGKNSKLILAAMIFSVAMMFIDQTIVAIAIPTIQDDLDLS
                     STGVQWIVNGYLLSLSALFAFGGRLADVLGHRRMLVIGILIFTTASALCGATPDGSFD
                     EAWLITFRLIQGAGAALLFPAALAIVLGAFPIRERGRAMAIFFAITGGLTAIGPLAGG
                     YLTEWTWRAIFWVNVPVALIALFLIWKSKPANVKHPAPIDFRGTVLITGGMSLLVLGL
                     QQAAVWGWGSVATWACIAAGIAILVFFVFYQLRIENPLLRLEVFRDRAFSVDNIILFL
                     LMIPFVPLFFFASMYAQISLGESASETGLYLLIFFAGFAIASQWGGKALDKTGARTAV
                     VPGCALAAVGFYLWGESLTDLSVDEQWYWIVMAGAGVGLVLSPANTDALNRVPASRYG
                     EATGITQTVRNFGSSLGLAILGSVLTLQNTANLEESAEEAGASAEVGKEIANSIAHSS
                     GAGEGGSEQVSGAAAKVVEAIPHDFALATQTVFYCFAGVMAVAFVVALKAMPGGKVDE
                     IPEEPGAGGSPPAGA"
     gene            6493..8460
                     /locus_tag="VFB52_01425"
     CDS             6493..8460
                     /locus_tag="VFB52_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05023.1"
                     /translation="MLGLLFVGVSPAAEGERLPTVAKETRSPVETRAAERLARKFAPI
                     THLREEQEPPCEKSAEQYLPTSVETVLGNRDVYLEQYVEGQGLEELVRAPTAADIAGL
                     DGDHYLNLRGQVLADTCVYARDFRDLERAGKAPALTYAHIAREPGHPGFALQYWFFWY
                     FNQFNDLHEGDWEGMQVTFEAPSPAKALARGEEPSEIILFQHAGGERAAWNDSKVQKE
                     GTRPLVYSAAGSHATFYDSAVYVENGQQGSGLGCDNTSEPLEEVPLQAVLIPDLIPRH
                     GPFQWLSYEGRWGEREKGYNNGPTGPATKTVWREPFAWMAQQRTTSPRLPGGSIVGPQ
                     VTAAFCGVVAVASELINLDAKSRPAAIATVLAILLVIALFIGLTRWGPIDLENLRARR
                     TFGQIVRSARQLYGRYWHVLVPIALTAIPIVGGVNLIGEQFHGNNGLDDAAGRAGLNL
                     AFADLIETLALPAAQAVVAAIVIVLVRLLVEGQQVGFRTCWGGMRQRFWRVVFAQFLA
                     SLGILALLVSVIGIPWAVNKLVAWRFVQQEVIFTDKKIREAFHGSSDLVRGRWWHTVR
                     LALFLTMISVVAGPLASFALIFTSLPLIWVNLLGALVFSLMIPYVALGDTLLYFDLQE
                     RAEAEGIKPGRSWKLWRPRSFGRVVAAPAVG"
     gene            8471..9067
                     /locus_tag="VFB52_01430"
     CDS             8471..9067
                     /locus_tag="VFB52_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZO05024.1"
                     /translation="MKLLAFSDLHRDLDQAAKLTEMSAEADVVIGAGDFASIHEGLEE
                     TIAALGGIETPTVLVPGNNETADALRAAAGSWAAATVLHGEGTTIDGVEFFGLGAGVP
                     TTPWDWSFDLSDEEATAMIAPCPQGAVLVLHSPPQGHCDSSADGTHFGSPALLAAIEE
                     KQPRLAVFGHIHEAWGCSSEVGATPLRNLGPTGTWIEL"
     gene            9095..>10257
                     /locus_tag="VFB52_01435"
     CDS             9095..>10257
                     /locus_tag="VFB52_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05025.1"
                     /translation="MESSGPKRAGAILATLILVAAVANLNLAVANVALPDIGQAFDAG
                     QTELNLVAVGYSLGLAASVLYLGAVGDRYGRKSLLLLGMALSLPFAALAALAPTIEVL
                     FLARVLGGVAAGMAYPTTLALITALWSGQARTRSIALWSALGGGISALGPLIAGALLE
                     GFDWWSVFIVTAPLAALALVLAWRLVPAHVNETTERVDHLGGIISIAFVGTMVLAINF
                     APVPGKGALVIGLAAVAVAGGAAFFLRQRRARVPLYDLHVAARRTFWVAAGAGLVVFG
                     TLMGAMFIGQQYLQNVLSYSTVESGAAILPAAILMVLIAPRSAKLVETRGSRFTLLVG
                     YVCCLLGFIVMLLLWSDGQSYWAVGLGYALIGAGVGFAGTPASHSLTGSVPVTR"
BASE COUNT         1412 a         3524 c         3586 g         1735 t
ORIGIN      
        1 cgcgtagacg ccggtgttgc cgccggtgat cgtcgccggg gaggcgaacg tcggttcttt
       61 cgccaccggg acctgcccgc tggccgaggt ggccgagccg aacgagttcg tcgctttcac
      121 cgtgcagacg actttcttgc cctggtcggc ggcgaccggc gtgtaggtcg gggagtcggt
      181 cccgaccgga gccccggcga ccgtccaggc gtaggtgtag gtcggcaccg ggtaggccgg
      241 agccgctccc gtgctgcagg tcagcggttt gccaaccgag gcgtagccgc tgaagccggt
      301 gatcgccgga gccgccgtga agtgcggttt gccttcgacg agcactttga ctttgacttc
      361 gctggtcagg ttgttggcgt cgaccgcgac ggcgctgatc gtgacctcgc ccgcgtagcc
      421 ggcggtcggg gtccaggagc cgctgtagac gcgctcgccg agcagcggcg gggtcggcgc
      481 cgtggagacg gtgccgccgt tggcgcggaa ttcaaccgtc cgcaccccga agtcgtcgcg
      541 ggcgaccgcg gcgagcggga tcgaggtgcc cggcgcgaag accgtacctt ccaccggttt
      601 cacttcccac tcgatgatcg gcagctgatc ggcgatcggg gcgagcgtgg gccccgccgg
      661 cgccgtcggc atggtcggct cggtgtcgac gttggggccg accagcggca caccggcgct
      721 cgtcgtcgtg ccccagaagt tgcctcccgc tttcagcgag gccgccgccg cggagccgtc
      781 gaaggcgacg ttggtcgccc cgtaggcgtt ggcggtgagg tcgttgcggt cggcttcgct
      841 gccgccgatc acgctgttga ccaggtcgac gccgtgcatc tcgaccgcgg cggcgctgcc
      901 gtcggctttg ccgacttcgg cgatcttgct cttagtcagc ttcaccgtgg cgccgctgcc
      961 ccaggcctcg accccggtct gaccgatcgg ctgcacgcca ccagccccgt tgatccgggt
     1021 gttgacgatg ttcgccgtca ccgtcgattt gttgccgacc ggttcggtgc cgctgaggcg
     1081 ccggctgtcg acgaggacac cggtcgcgtt gaacccggag gtcgtgctgc cgaactggct
     1141 gccggtgatc gtcgtcgtgt tctgggtctg ctcgatcgtg ttggcgacaa tcaccgcgtt
     1201 cccgtattgg gagagcgggt tcggcaccga ttcgatttcg ggcgcgtagg cgccggtgcc
     1261 cggcagcggt ttcgggttgt tgccgccggt cgtcggcacg atgccttcga ctttgacgct
     1321 ggagatcgtg ccggtgacga cgtcgatcat cgcgatcccg gcctcgaccg ggacgttgcc
     1381 ggcgcgcacc gtgaggcccg agatcgagac gtcgtagagc tcgatcaggc ggcggcggac
     1441 ggtgatcacg ttgccgtact cgtcacgcgg gttcggcgtc gcctgggcca tcgagccgct
     1501 gccctgggtc ggctggatga acactttgtc cgagccggcc ccggcgaggt tgatgttctt
     1561 ctcgatcttc agccccgagg acggtgccgg gccgcccggc acgttgtagg tgccggcgca
     1621 gattttcacc gtgtccccgt tggccgcgac ggcaaccgcc gcggtcagcg aggtctcgtc
     1681 ggccagcggg cactggactc ggtcgtcgtc gaccgtgatc gtcagcgccg aggcaaggga
     1741 cgggagcgcg gccagcccga gcagggcgac cacaagcgtg gcgatgaatt tgcgggaggt
     1801 cacgagccca cctcgattcg gctgaacttg acggaggaca cgttgccggc gccgtcaatg
     1861 gcgaaggcgg cgactttgta cttgcccgct ggtaaacgtt ttggcggtcg cagcttccag
     1921 tgcttcttgc cgctggcgac gaccgagagc ggcgtcgtgc agggggtctt cttcatcagc
     1981 ctgccgtcgg cgccgaggaa gcggcacttc ttgccggcct ctttgaacac cgagacctgg
     2041 acctgctcga cggtgcccgg cgaggccttc ttggcggcac ccgcggcaca gcccttgtcg
     2101 ccggccttgc cggtgacaac gagcttcttg ccgctcttca ccgccttgat cgtcaacgtc
     2161 ggcttcttct tgtcgacgca gtagccgggc tcggtggcga agcgcggggc gcccgtcttg
     2221 agcgcttcga gcttcggcag ctccgggtcg ctgggcagcg gcttcagcgc cgcgaacggg
     2281 gtcgccgccg tctccggcgt ctcctggaag gcgacgtcgg agaactggat cgccccgctc
     2341 gggttgactc cggcggtgcc gaagcggaac tcgatccggt cgagggcggc gacgtcgacg
     2401 ccgtcggcgg cgaaggtcgc cagcggcgcg atgaactggt tgagaacgac gtgctggctg
     2461 ttgttggggg tcgtccccag cgccttctcg acgccgcccg agaagaactc ggtcagcttg
     2521 tgggcgctct cctgcccttc gcgatcggtt accaccaccc agaagtcctg cgtcgcgctg
     2581 cgcgggtcga agtagttgcc gaccgggttc agcgcctcgt cgttggactg gaaggcggcg
     2641 cggaagacca ggttgtcgta cggcgtcgcg ttacgggcgt cgccgcgcag ggtcgtcgtc
     2701 agcgtcgccg ggccgttcca ggccagggtc agctgcggtc cccaggactg gatcgcctgg
     2761 ttggccgtgt aggtggtcgt gctcggcggc gtgccgagga cccggtccgg cccttcgcag
     2821 gcgaccagtg ccgtcgtcgt ctgcgtggcc ggcgggttcg ccatcacgtt cgcgaacggg
     2881 tccgggttgc accactggaa ggtgctgaac ccggtcccgg agtacatccc gcccacttcg
     2941 ttgggcgcga tcgggctggc cgtggtcggc cgcacgccag ggcccgggcg gaggatgtcg
     3001 agccgttcac cggccggcgc gatgtaggtc tgcttgacct ggtcttcgca gaacggcacg
     3061 tgggtcggcc cggtcgcgca ggccgaggcg ggcaggccgg cttcgccggt cagccacggc
     3121 atcaggtcgg tttcgccacc gatgtaggcg cgcaggaagg tcggcatcgt cgccagaccg
     3181 gtcttgcgct ggtcctcggg ggtcagccgg gctttgccgg taccgaaggc ggttccgcag
     3241 gcgatgtcgg agccgccgcg gtcgctgctg ggccagcggg tgttgtagaa gttgtggttc
     3301 gccccctcga cgtagtactg gatcttcgcg taggggtcat cgggcgccac gtacttgccg
     3361 cgctcgaagg cgttggcgcc ggcgatcgtg gtcacgtcac cgtcgcaggc cggaagcagg
     3421 gtcgcgtaat tggtgccgta cggcatctgc ttgttgcggt cgatcggcgc cagcgagaag
     3481 accgcctgca ggttgtagcg gcggcccggg gccgggcggg cccggttgaa cgggatgaac
     3541 tccgtcaccc cctcgccgcc gcgcgagtga cccatgatcc cgaccttgct gaagtcgagc
     3601 ttgccggcca gctgggtgcc gatgccgggc tggccgttgg cctgctggat cgaggtcggc
     3661 gccggccccg gcacgccctc gttcagggag tagaggatgt cgagcgtgtt gccgatgatc
     3721 tcggcgcggg cgaatgctcc ggcgtccatc acgctggtcc cgtcgaaggc gacgagcgct
     3781 ccggccgagg gcgagatcac cacgtacccc caggaggcga gcaggtcgga gatgtagttg
     3841 tagccgcccc agctcggcca gaacccggtc accgagccgg cgtcctcgac cgtctcgggg
     3901 cccgaggggc cggcgggatt gaccggcacg ttcgggttcg ggcagagacc agtgaggaac
     3961 gactgaccgc cgttgtcggt tccggtcgtg cccttgcagg cgctgtggcg cccgtgcaag
     4021 aagaggatca ctttgtaggg gcccgggtgc gatttcggcc aggtgatgct cccctccagc
     4081 ggctgcggga aggtctggcc ggggcatttg gagctcgagc cgtattcgtg ggtggacccc
     4141 gagccggttt cgcaggtccc cgaccccggc ggaagcacgc cgctcgacgg cacggtcagc
     4201 tgcagggtgc cggcgtcgta gtaagtgcga ttgaccccat agggacccat ctgcagcgga
     4261 tctggcggca ccgcgagagc agtgaccggc agcacgacgg atacgagcgc ggccagcgag
     4321 accgcgagga cgagcacctt cttcaatttg ggtcccttct gcctcaggac ctggggagat
     4381 ccgaggggaa gcttgggttg ggaggagcaa cctaggctcg ggtgaggggc gccctaacta
     4441 gacagcggtc caaagatccc agctcaggaa tccccctctg gacatggacc gggagtcgac
     4501 ggcggctgga ccggcagtcg aagacgcccg ctatggggct gtttcacagc gggccagcga
     4561 ggcgggcgct agctggtcgc caggccgatg gcgagcagcg ggaaggcgag catgacgccg
     4621 aggaaggcgc cgatcgcggc gcccgaacca aggctggcgc tgcgtgagcg ggctccggag
     4681 atgacgccga cgaggaagat gaaggcggcg attccgaggc cgatccagaa gacgggaggc
     4741 attgcgcggg cgagactatc ggattccgcg gagctgccga taagccctcc gtatgggcgt
     4801 tgccgagcag gaatatcgac cttcgtgaca aacgccacgg catgagcgaa acgggcggta
     4861 aaaacagcaa gctgatcctc gcggcgatga tcttctcggt cgcgatgatg ttcatcgacc
     4921 agacgatcgt cgcgatcgcg atcccgacga tccaggacga cctcgacctc tcctcgaccg
     4981 gggtgcagtg gatcgtcaac ggctacctgc tctcgctctc ggcgctgttc gccttcggcg
     5041 gccggctcgc cgacgtgctc ggtcaccggc gcatgctggt gatcggcatc ctcatcttca
     5101 ccaccgcctc cgccctctgc ggcgccactc cggacggctc ctttgacgag gcctggctga
     5161 tcacgttccg gctgatccag ggcgccggcg cggcgctgct cttcccggcg gcgctggcga
     5221 tcgtgctcgg cgccttcccg atccgcgagc gcggccgcgc gatggcgatc ttctttgcga
     5281 tcaccggtgg gctgaccgcg atcggcccgc tcgccggcgg ctacctgacc gagtggacct
     5341 ggcgcgcgat cttctgggtc aacgtcccgg tcgccctgat cgcgctcttc ctgatctgga
     5401 aatcgaaacc ggcgaacgtc aagcacccag ccccgatcga cttccgcggc acggtcctga
     5461 tcaccggcgg catgagcctg ctcgtgctcg gcctccagca ggccgccgtc tggggctggg
     5521 gcagcgtcgc cacctgggcc tgcatcgccg ccgggatcgc gatcctcgtc ttcttcgtct
     5581 tctaccagct gcggatcgag aacccgctac tgcggctcga ggtcttccgc gaccgcgcct
     5641 tcagcgtcga caacatcatc ttgttcctgc tgatgatccc gttcgtcccg ctgttcttct
     5701 tcgccagcat gtacgcgcag atctcgctcg gcgaaagcgc ctcggaaacc ggcctctacc
     5761 tgctgatctt cttcgccggc ttcgcgatcg cctcgcagtg gggcggcaaa gcgctcgaca
     5821 aaacgggagc gcggacggcg gtcgtgccgg gatgcgcgct ggccgcggtc ggcttctatc
     5881 tctggggcga atcgctgacc gatctctcgg tcgacgaaca gtggtactgg atcgtgatgg
     5941 cgggcgccgg ggtcgggctc gtcctctccc ccgccaacac cgacgcgctc aaccgggtgc
     6001 cggcgagccg ctacggcgag gccaccggga tcacccagac cgtgcgcaac ttcggctcca
     6061 gcctcgggct cgcgatcctc ggctcggtcc tcaccctgca gaacaccgcc aacctcgaag
     6121 agagcgccga agaagccggc gcctcggccg aggtcggcaa ggaaatcgcc aactcgatcg
     6181 cgcactccag cggcgccggc gaaggcggct ccgaacaggt cagcggcgcg gccgccaagg
     6241 tggtcgaagc gatcccgcac gacttcgcgc tcgccaccca gacggtcttc tactgcttcg
     6301 cgggcgtgat ggcggtcgcc ttcgtcgtcg ccctgaaagc gatgcccggg ggcaaagtcg
     6361 acgagatccc cgaggagccg ggtgccggcg gctcccctcc ggctggcgcc tgacggccgg
     6421 cgccagagga tgtacgttgc cggggtgagg tcccggctcg ctcgtccctg gctcctactg
     6481 acgagtctct gtttgctcgg gctcctcttc gtcggcgtct ccccggcggc cgaaggtgaa
     6541 cggctgccga ccgtcgccaa ggaaacccgc tcgccggtcg aaacgcgggc ggccgaacgg
     6601 ctggcgcgca agttcgcgcc gatcacccac ctgcgcgaag agcaggagcc gccctgcgaa
     6661 aagagcgccg agcagtacct gccgaccagc gtcgagacgg tgctcggcaa ccgcgacgtc
     6721 tacctcgagc agtacgtcga aggacaaggt ttggaagagc tcgtacgggc gccgaccgcg
     6781 gctgacatcg ccgggctcga cggcgaccac tacctcaacc tgcgcgggca ggtgctcgcc
     6841 gacacctgcg tctacgcgcg cgacttccgc gacctggaac gcgcgggcaa ggccccggcg
     6901 ctcacctacg cgcacatcgc ccgcgaaccg ggccaccccg gcttcgcctt gcagtactgg
     6961 ttcttctggt acttcaacca gttcaacgac ctgcacgagg gcgactggga gggcatgcag
     7021 gtcaccttcg aagcgccgag cccggcgaaa gccctggccc gcggggaaga accgagcgaa
     7081 atcatcctct tccagcacgc cggcggcgag cgcgccgcct ggaacgactc gaaggtgcag
     7141 aaggaaggga cgcggccgct ggtctactcg gcggccggct cgcacgcgac cttctacgac
     7201 tccgccgtct acgtcgagaa cggccagcag ggctccgggc tgggctgcga caacacctcc
     7261 gaaccgctgg aagaagtgcc gctgcaggcg gtcctgatcc cggacctgat cccgcgccac
     7321 ggccccttcc agtggctcag ctacgaaggc cgttggggcg aacgcgaaaa gggctacaac
     7381 aacggcccga ccgggccggc gacgaagacc gtgtggaggg aaccgttcgc ctggatggcg
     7441 cagcagcgga cgaccagccc gcgcctgccg ggcggctcga tcgtcggccc gcaggtcacc
     7501 gccgccttct gcggggtcgt cgccgtcgcc tctgaactga tcaacctcga cgccaagtcg
     7561 cgcccggcgg cgatcgccac ggtgctcgcg atcctgctgg tgatcgcgct cttcatcggc
     7621 ctcacccgct gggggccgat cgacctggag aacctgcggg cgcgccgcac cttcggccag
     7681 atcgtccgca gcgcgcgcca gttatacggg cgctactggc acgtgctggt gccgatcgcg
     7741 ctgaccgcga tcccgatcgt cggcggcgtc aacctgatcg gcgagcagtt ccacggcaac
     7801 aacgggctcg acgacgccgc cgggcgcgcc ggcctcaacc tcgccttcgc cgacctgatc
     7861 gaaacgctgg cgctgccggc cgcgcaggcg gtggtcgcgg cgatcgtgat cgtgctcgtc
     7921 cgcctcctgg tcgaaggcca gcaggtcggc ttccggacct gctggggcgg catgcgccag
     7981 cgcttctggc gcgtcgtctt cgcccagttc ctcgcctcgc tggggatcct ggcgctgctg
     8041 gtctcggtga tcgggatccc ctgggcggtc aacaaactgg tcgcctggcg cttcgtccag
     8101 caggaagtga tcttcaccga caagaagatc cgcgaggcct tccacggcag cagcgacctg
     8161 gtccgcggcc gctggtggca cacggtgcgg ctggccctct tcctcaccat gatcagcgtg
     8221 gtcgccgggc cgttggccag cttcgcgctg atcttcacct cgctccccct tatatgggtc
     8281 aacctgctcg gggcgctggt cttctcgctg atgattccct acgtggcgct cggcgacacg
     8341 ctgctctact tcgatctgca ggagcgggcc gaggccgagg ggatcaagcc cgggcgctcc
     8401 tggaagctct ggcggccgcg ctcgttcggg cgcgtggttg ccgccccagc ggtcggatag
     8461 gttttccgct gtgaagctgc ttgcgttcag cgacctgcac cgcgacctcg accaggcggc
     8521 gaagctgacc gagatgtcgg cagaggccga cgtggtgatc ggggccggcg acttcgcctc
     8581 gatccacgag ggtctggagg agacgatcgc ggcgctgggc gggatcgaga cgccgaccgt
     8641 gctcgtgccc ggcaacaacg agaccgccga cgcgctgcgc gccgccgccg ggtcctgggc
     8701 cgccgcgacc gtgctgcatg gcgagggcac gacgatcgac ggggtcgagt tcttcggcct
     8761 cggcgccggc gtcccgacca cgccctggga ctggagcttc gacctctccg acgaggaggc
     8821 gacggcgatg atcgcgccct gccctcaggg cgccgtgctc gtcctccact cgcccccaca
     8881 gggccactgc gactcgagcg ccgacggcac ccacttcggc agcccggcgc tgctcgccgc
     8941 gatcgaggag aagcagccgc gcctcgcggt cttcggccac atccacgagg cctggggctg
     9001 cagcagcgag gtcggcgcga cgccgctgcg caacctcggc ccgaccggga cctggatcga
     9061 gctttgatga gctgccccgc gggggtctga agcggtggaa tcgagcggcc ccaagcgcgc
     9121 cggtgcgatc ctcgcgacgc tgatcctggt cgccgcggtc gccaacctca acctcgcggt
     9181 cgccaacgtc gccctgccgg acatcggcca ggccttcgac gccggccaga ccgaactcaa
     9241 cctggtcgcg gtcggctact cgctcggcct cgccgcctcg gtcctctatc tcggcgcggt
     9301 cggcgaccgc tacgggcgca agtcgctgct gctgctgggc atggcgctct ccctcccctt
     9361 cgccgcgctg gccgcgctcg cccccacgat cgaggtgttg ttcctcgccc gggtgctcgg
     9421 cggcgtcgcc gccggcatgg cctacccgac cacgctggcg ctgatcacgg cgctctggtc
     9481 gggccaggcg cggacccgct cgatcgcgct ctggtcggcg ctcggcggcg gcatctcggc
     9541 gctcggcccg ctgatcgccg gtgccctgct ggaagggttc gactggtggt cggtcttcat
     9601 cgtcaccgcg ccgctggcgg cgctggccct ggtcctcgcc tggcggctcg tcccggccca
     9661 cgtcaacgag accaccgagc gggtcgacca cctcggcggg atcatctcga tcgccttcgt
     9721 cggcacgatg gtgctggcga tcaacttcgc cccggtcccc ggcaagggcg cactcgtgat
     9781 cgggctggcg gcggtcgccg tcgccggcgg agccgccttc ttcctccgac agcgacgagc
     9841 acgggtcccg ctgtacgacc tccatgtcgc cgcccgccgc acgttctggg tcgccgcggg
     9901 cgctggtctg gtcgtcttcg gcacgctgat gggggcgatg ttcatcggcc agcagtacct
     9961 gcagaacgtc ctctcctact cgacggtcga gtccggagcg gcgatcctgc cggcggcgat
    10021 cctgatggtc ctcatcgcgc cgcgctccgc caagctcgtc gagacccgcg gctcccggtt
    10081 caccctgctc gtcgggtacg tgtgctgcct gctcggcttc atcgtgatgc tcttgctgtg
    10141 gagcgacggc cagtcgtact gggcggtcgg gctcggctac gcgctgatcg gtgccggcgt
    10201 cggcttcgcc ggcaccccgg cgtcgcactc gctgaccggg tcggtccccg tcacgcg
//
LOCUS       DASRIG010000053         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_547572, whole genome shotgun sequence.
ACCESSION   DASRIG010000053 DASRIG010000000
VERSION     DASRIG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547572"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFB52_01440"
     CDS             <1..616
                     /locus_tag="VFB52_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05026.1"
                     /translation="VSSVSQLLEERLARVLSERDNRLRSLFIDRLGAEGKGRSALVTR
                     CGRVVAAYPKNWLGRRVAVGTDGNLVFPAGIEVEVEPLNGHNGALLVRGTSARAQPQR
                     AATTIEALPPRRVRVARGDWHVDLSPRHSEIITQLALHPDGLSNEEIRELLYEAGAKS
                     VTVRAEISRLRKLLGPILLSNPYRLEQPVRADPAELRELLDSSR"
     gene            complement(693..1844)
                     /locus_tag="VFB52_01445"
     CDS             complement(693..1844)
                     /locus_tag="VFB52_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05027.1"
                     /translation="MSKKLTEKGLLGLAAIAALVLALGLAACGGSDDDSSGTSGSSEP
                     ASTEPAGGEEEKQQVSLAMELILTGQSFGVEIQEGIEAFAAEDGATSVEIAGPPQVNP
                     EVAQKQASDLLATSPEGMGFAPFPPELWTRTAKTIDEQVGPHVLNFNERPSSELDTVD
                     AATIKTFVGTADRTLARESTEETIKLAGLPPSTTGEAIIGQCVPQEAGVLAMRTEGVK
                     EVLAEMLPKVDPVVFDSKPTGPEGLVAWTNQLAANPDPVLAIGVCTEDGQSLNKVMTE
                     KGYDFPAGAMDVAPGTITGLENGNLLVTVAVNWWLQGYTSTRMLAESVRGAELPEGWI
                     NTGQLVVTKDNVAQLEEKINEPDKYFAPQVEELFANGMPAAAPMADSFK"
     gene            complement(1951..>2771)
                     /locus_tag="VFB52_01450"
     CDS             complement(1951..>2771)
                     /locus_tag="VFB52_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO05028.1"
                     /translation="RPAGGAELVDEYLEPLSVLGLGRLLSLGDLDADTLRRWFKELQA
                     GSGNTAGEEAGFAVSDNAVAEIEAHVDPILDRLEADPDDSLLSHMLFGGRPEGSPRPR
                     AEIYPSYLIVISGGMREPSHAGASTLLGLLTNSDQLAAVVADPALVPRAIVEGLRWIS
                     PIAQAVRSPFAAAELAGVEIPAGALVNAVLASANRDERKFERPDEFDFTRSPNAHMSF
                     GHGAHFCAGNFFARQLLRIGLEELLAAFPAMRLERVPPVRGFMFRAPVELRVSW"
BASE COUNT          396 a          938 c          977 g          460 t
ORIGIN      
        1 ggtctcctcg gtctcgcagc tgctcgagga gcggctggcg cgtgtcctca gcgagcgcga
       61 caaccggctg cgctcgctgt tcatcgaccg gctcggcgcc gagggcaagg ggcgcagcgc
      121 cctggtcacg cgctgcggcc gggtggtcgc cgcctacccg aagaactggc tcgggcgccg
      181 ggtcgcggtc ggcaccgacg gcaacctcgt cttccccgcc gggatcgagg tcgaggtcga
      241 accgctgaac ggccacaacg gggccctgct ggtccgcggg acctcagcgc gggcgcagcc
      301 gcagcgggcg gcgaccacga tcgaggcgct gccgccgcgc cgggtgcggg ttgcccgcgg
      361 cgactggcac gtcgacctca gcccccgcca cagcgagatc atcacccagc tcgccctcca
      421 ccccgacggc ctcagcaatg aggagatccg ggagctgctc tacgaggcgg gcgcgaagtc
      481 ggtgaccgtg cgggcggaga tctcgcggct gcgcaagctg ctcggcccga tcctgctcag
      541 caacccctac cggctggagc aaccggtgcg cgccgaccca gccgagctac gcgaacttct
      601 cgactcctcc cgctgaagcc aggcgacgcc accgagaacc caggggttca gagggactcg
      661 gtggcgtcgc cgttgggagg agggtcagag tgttatttga acgaatctgc catcggcgcc
      721 gctgcgggca taccgttggc gaacagttct tcgacctggg gggcgaagta tttgtccggt
      781 tcgttgatct tttcttccag ctgggccacg ttgtctttgg tgaccacaag ctggccggtg
      841 ttgatccacc cttccggcag ttccgcgccg cgcacggatt cggcgagcat gcgggtcgag
      901 gtgtagccct gcagccacca gttcaccgcg acggtgacca ggaggttgcc gttttccagg
      961 ccggtgatcg tgcccggggc gacgtccatc gcgccggcgg ggaagtcgta ccccttttcg
     1021 gtcatcacct tgttcaggct ctggccgtct tcggtgcaga cgccgatcgc gagcaccggg
     1081 tcggggttcg ccgccagctg gttggtccag gcgacgaggc cttccggccc ggtcggtttc
     1141 gagtcgaaga caaccgggtc gactttcggc agcatctccg ccagcacttc cttgacccct
     1201 tcggtgcgca tcgcgagcac gccggcttcc tggggcacgc actggccgat gatcgcttca
     1261 cccgtggtcg agggcggcag gcccgcgagt ttgatcgttt cttcagtcga ttcgcgcgcc
     1321 agggtgcggt cggcggtgcc gacgaaggtc ttgatcgtcg ccgcgtcgac cgtgtcgagt
     1381 tctgaggacg ggcgttcgtt gaagttgagg acgtgcggac cgacctgttc gtcgatcgtt
     1441 ttcgccgtac gcgtccagag ctcgggcggg aacggtgcaa agcccatccc ttccgggctg
     1501 gtcgcgagca ggtcggacgc ctgcttctgg gcgacttccg ggttgacctg cggcgggccg
     1561 gcgatctcga cgctggtggc gccgtcttcg gccgcgaacg cttcgatgcc ttcctggatc
     1621 tccaccccga acgactggcc ggtgaggatc aactccatcg ccagcgagac ctgctgcttt
     1681 tcttcttcgc cgccggcggg ttcggtgctc gctggttcgg acgagccact ggtgccgctg
     1741 gagtcgtcgt cgctcccccc gcacgcagcc agcccaaggg ccaggaccag cgccgcgatc
     1801 gcggcgagcc cgagcagacc cttctcggtc agtttcttac tcattcagtt gcattcctct
     1861 cctgtaggtg accgctgact tacccacgct gaacctatgt gggtgaatcg ccgctgtcgc
     1921 ctacgagtga gggttgcgaa gaggttgcaa ctaccagctg acccgcagct caaccggggc
     1981 gcggaacatg aacccgcgca ccggcggcac gcgctccagc cgcatcgccg ggaaggcggc
     2041 gagcagctcc tcgaggccga tccgcaggag ctgacgggcg aagaagttgc cggcgcagaa
     2101 gtgcgcgccg tggccgaacg acatgtgtgc gttcggcgag cgggtgaagt cgaactcgtc
     2161 ggggcgctcg aacttccgct cgtcgcggtt ggccgaggcg agcaccgcgt tgaccagggc
     2221 gccggcgggg atctcgaccc cggcgagctc ggccgcggcg aacggggagc ggaccgcctg
     2281 cgcgatcggc gagatccagc gcagcccctc gacgatcgcg cgcgggacca gggccgggtc
     2341 ggcgaccacg gccgcgagct ggtcggagtt ggtgagcagc ccgagcaggg tcgaggcgcc
     2401 ggcgtggctg ggctcgcgca tcccgcccga aatcacgatc aggtagctgg ggtagatctc
     2461 cgcgcgcggc cgcggcgagc cctccgggcg gccgccgaac agcatgtggg agagcagcga
     2521 gtcgtcgggg tcggcttcca gccggtcgag gatcgggtcg acgtgggctt cgatctcggc
     2581 gaccgcgttg tcggagacgg cgaacccggc ctcctcgccg gcggtgttgc cgctgcccgc
     2641 ctgcagttcc ttgaaccaac ggcgcagggt gtcggcgtcg aggtcgccga ggctgagtag
     2701 ccggccgagc ccaagcacgc tgagcggctc caggtactcg tcgaccaact ccgccccgcc
     2761 ggccggccgc a
//
LOCUS       DASRIG010000054         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2897963, whole genome shotgun sequence.
ACCESSION   DASRIG010000054 DASRIG010000000
VERSION     DASRIG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897963"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..772)
                     /locus_tag="VFB52_01455"
     CDS             complement(107..772)
                     /locus_tag="VFB52_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05029.1"
                     /translation="MNTLKRHLSVANVLSVVAIFIALSASAVAATKLGPGQVKEVNIA
                     NEAVSNPKLKNLGITSSKIANGTIVTGKLKGSSVIAGKLGKEAVTAGKIKKKAVGTAA
                     LAPESVTGAKIGSEAITAAKVSTSFYSTLVKGTVYPAGVETATNEVEEKTAIAACPTG
                     KQAIAGGARVNGTLKEVAITGSQPYFNGTAHSGWEAFARDIVPGGGAGPWSLTVFAVC
                     AEL"
     gene            complement(842..1051)
                     /locus_tag="VFB52_01460"
     CDS             complement(842..1051)
                     /locus_tag="VFB52_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05030.1"
                     /translation="MIEVSRTLMKSEPELAELIAAVEGVEVTMAEKGFGTRVEIRAAE
                     ETGLVAADLEAVLDSLAEPQRRPFS"
     gene            complement(1250..2893)
                     /locus_tag="VFB52_01465"
     CDS             complement(1250..2893)
                     /locus_tag="VFB52_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZO05031.1"
                     /translation="MNPPNGSATEDAHRTIAPVVGPDDERVFTDSGIEIEALYDEGDV
                     APGLTELLGEPGAPPFTRGIHDGMYRDRLWTMRQYAGFASPEDTNERYRYLIEHGSTG
                     LSMAFDLPTQLGRDSDDPLCHGEVGRTGVPIDTIEDMRICFDQIPLDGVSTSMTINAP
                     AAILLLLYELVGEEQGVPSQKLRGTVQNDVLKEYMARGNYIFPPAPTMRITTDLFEYC
                     HQNVPKWNTISISGYHIREKGCSAVQEVAFTLSNAIAYVESALERGLKIDEFAPRLAF
                     FFNCHNNVFQEVAKFRAARRMWSKIVGERFGAENPKSRQIRFHTQTGGVTLQAQQPEV
                     NIIRVALQGFAAVAGGTQSLHTNGFDEALALPTERSARIALRTQQVLAYESGVADTVD
                     PFAGSYFIESLTDEIELRAWELMEKVEELGGSVEALEFMQREIEESALSYHERYRSGQ
                     DIIVGVNKFVTDQVDDVDILKVDPEAEQRQLARLKTFKESRDQAAVDTQLDALRDVAR
                     GDGNLLHPIRSALKANASIGEVCGAMREVFGEYKGGAFF"
BASE COUNT          443 a          948 c         1026 g          535 t
ORIGIN      
        1 cggtgtcccg cgataccgcc ccggtgccca caaccggccc agaacgtgcg gagggcccgc
       61 tgctcgcggg ccctcccagg cacatctatg tgtcgcttcc gcgagcttag agttcggcgc
      121 agaccgcgaa cacggtcagc gaccaggggc cggcgccgcc acccggaacg atgtcgcggg
      181 cgaaagcctc ccagccgctg tgggcggtgc cgttgaagta cggctgcgaa cccgtgatcg
      241 cgacctcttt cagggtgccg ttgacacgag cgccgccggc gatcgcctgc ttgccggtcg
      301 gacaggctgc gatcgcggtc ttttcttcga cttcgttggt ggcggtttcg acgcccgcgg
      361 gatagaccgt ccccttgacc agggtcgagt agaacgaggt cgagaccttc gcggccgtga
      421 tcgcttccga gccgatcttg gcgccggtca cagattcggg agcgagcgcg gcggtcccaa
      481 cggctttctt cttgattttc ccggcggtga cggcttcctt gccgagtttc ccggcgatca
      541 ccgagctgcc cttgagcttg ccggtgacga tcgtcccgtt ggcgatcttc gaggaggtga
      601 tccccaggtt cttcagcttc gggttcgaca ccgcttcgtt ggcgatgttc acttccttga
      661 cctgcccagg gccgagtttg gtcgccgcga cggcgctggc gctgagcgcg atgaagatcg
      721 caaccacaga caagacgttg gcaaccgaga ggtgccgttt cagagtgttc actctccgga
      781 ctcgctttct ggggcggttc ccaccgcccg tcttaggggg aaatgcagat gcacgcttgg
      841 ttcagctgaa aggtcggcgc tgcggttccg ccaggctgtc gaggacagcc tcgaggtcgg
      901 ccgcgaccaa cccggtctcc tcggccgcgc ggatttcgac gcgggtccca aagcccttct
      961 cagccatcgt cacctcgacg ccctcgacgg ccgcaatcag ctccgccagc tcaggctcgg
     1021 acttcatcag cgtcctggaa acctcgatca tggcgtcgct tttcgggtca gctcccccaa
     1081 atccttcgat ggtcgacgtc gtgaaatcgt gcgaccaggg gtggccgtgg ccaagcaggc
     1141 caaggacgca gggagcctga atagcagcgc tattcaggcg actgaggacg aaggcatgcg
     1201 ccgcgccacg gacgcacctg ggcgtgcgag ggcgcgacgg cgcccatcgt tagaagaacg
     1261 cgccgccctt gtattcgccg aagacctcgc gcatcgcgcc gcagacctcg ccgatcgagg
     1321 cgttggcttt gagcgcggag cggattgggt gcaggaggtt gccgtcgccg cgggcgacgt
     1381 cgcgcagggc gtcgagctga gtgtcgacgg cggcctggtc gcgcgactcc ttgaacgtct
     1441 tcaggcgggc cagctggcgc tgctcggcct cggggtcgac cttgaggatg tcgacgtcgt
     1501 cgacctggtc ggtgacgaac ttgttgacgc cgacgatgat gtcttggccg gagcggtagc
     1561 gctcgtggta gctcagcgcc gactcctcga tctcgcgctg catgaactcc agcgcctcga
     1621 ccgagccgcc gagctcttcg accttctcca tcagctccca ggcgcgcagc tcgatctcgt
     1681 cggtcagcga ctcgatgaag taggagccgg cgaaggggtc gacggtgtcg gcgacgccgg
     1741 actcgtaggc gagcacctgc tgggtgcgca gggcgatccg ggcggagcgc tcggtcggca
     1801 gcgccagcgc ctcgtcgaag ccgttggtgt gcagcgactg ggtgccgccg gcaaccgcgg
     1861 cgaagccctg cagggcgacg cggatgatgt tcacctcggg ctgctgggcc tgcagggtca
     1921 ccccgccggt ctgcgtgtgg aagcggatct ggcgcgattt cgggttctcg gccccgaacc
     1981 gctcgccgac gatcttcgac cacatccgcc gggcggcacg gaacttggcg acctcctgga
     2041 agacgttgtt gtggcagttg aagaagaagg cgagccgtgg cgcgaactcg tcgatcttca
     2101 ggcctcgctc gagcgccgat tccacgtagg cgatcgcgtt ggagagcgtg aacgcgacct
     2161 cctgcacagc cgagcagccc ttctcgcgga tgtggtaccc ggagatcgag atcgtgttcc
     2221 atttcggcac gttctggtgg cagtactcga acaggtcggt ggtgatccgc atcgtcggcg
     2281 ccggcgggaa gatgtagttg ccgcgcgcca tgtactcctt gagcacgtcg ttctggaccg
     2341 tgccgcgcag cttctgggaa ggcacgccct gctcctcgcc gaccagctcg tagagcagca
     2401 gcaggatcgc ggcgggcgcg ttgatcgtca tcgaagtgga gacgccgtcg agcgggatct
     2461 ggtcgaagca gatccgcatg tcctcgatcg tgtcgatcgg gacaccggtg cggccgacct
     2521 cgccgtggca gagcgggtcg tcggagtcgc ggccgagctg ggtcggcagg tcgaaggcca
     2581 tcgagagccc ggtcgagccg tgctcgatca ggtagcggta gcgctcgttg gtgtcctcgg
     2641 gcgaggcgaa gccggcgtac tggcgcatcg tccagagccg gtcgcggtac atcccgtcgt
     2701 ggatcccgcg ggtgaacggc ggcgcgcccg gctcgccgag cagctcggtc aggcccgggg
     2761 cgacgtcgcc ctcgtcgtag agggcttcga tctcgatccc ggagtcggtg aagacgcgct
     2821 cgtcgtcggg gccgacgacg ggtgcgatcg tgcggtgggc gtcctcggtg gcggagccgt
     2881 tcggtggatt catcgcgtcc agagaatatt gacctgcaga ttctcgccca gcgcgaccct
     2941 ggctggccgg cc
//
LOCUS       DASRIG010000055         8413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1586863, whole genome shotgun sequence.
ACCESSION   DASRIG010000055 DASRIG010000000
VERSION     DASRIG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586863"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFB52_01470"
     CDS             <1..308
                     /locus_tag="VFB52_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZO05032.1"
                     /translation="EQITAFAPTAFKLETKLPVSVTTGAGIATGAGTYSYVGCKVPTL
                     ANKKLKPAKRALKKAGCKLGNVTLRYGATARTGKVLSQHPSAGTIRLPGTKVKLTLY"
     gene            337..407
                     /locus_tag="VFB52_01475"
     tRNA            337..407
                     /locus_tag="VFB52_01475"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:369..371,aa:Cys,seq:gca)
     gene            618..2564
                     /locus_tag="VFB52_01480"
     CDS             618..2564
                     /locus_tag="VFB52_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZO05033.1"
                     /translation="MADGETAVAEKKGVQPPPTEKVRDDGFLPGPMDRRSFLVRTGGA
                     AAVAGAAAAFGPAASVLAAPKSPAASTAASPLPSAQAVLADGRDLVDHEVVELAALLQ
                     AGEVSSVELTQAYLDRINSLNGPFETYADNNHYNGFVRIDEAGALAAARTADEALAAG
                     RRDGTAVPFLCGIPMGFKDSIGTAGFAQQNGTVAYLGNVAFEDSTPVAYLRAKGVVPL
                     GITVCSAFSGTIAGTFSGNAWDLDYVPGGSSQGSGVATIARLAAATLGEETGGSIIFP
                     AACNGASAIKPSQGLVSTAGVMPLSPGYDTIGPICRSGRDSALIMNALIGEDPFGDPQ
                     TLAVPAGFPSLSLTPRIGSKPLAGITIGINKTDWLTVAVGETTETKRNVDPQSLYGAP
                     HIAAFERLRGELEAMGAKVVDFNGVDFLEFSEEANPYYNNPDALMTIEGVNITPANAV
                     VSPNRYDIRYTEAVREFAETRPAGQATELLKQYGRQEDWDKAILQQSAITTAVRREGE
                     RRRRKWAANYKAAFDAAGVDFMLVMTLGDKPALRADSRYPCRRNNQLANSMSFPMVSF
                     PIGYDPEGLPICAQFKGPRFSEPQLVQAMIDYQARHSEHHTARPPDYAEKASLKLSRA
                     RSLALETEDLSLSSDPLVSEENFR"
     gene            2561..3163
                     /locus_tag="VFB52_01485"
     CDS             2561..3163
                     /locus_tag="VFB52_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05034.1"
                     /translation="MSKMTVDWDRGEQIGFMPEASEVVYPEPILTVEWVPLTTATIID
                     YDPADLLVTPGAGPDVPPGGGPGEGGGPGGGDGPGAPTHYGRPQLVVPKRRSEKSFLE
                     HGLRIGIDAPAPARVRAVLKGKLPRTKTQPSRWLNLTKVVTQDTGAGEGALVLDPSRA
                     GRRALHRLEDSVRAELILVVRYANGQDPIRIERGLTIVDS"
     gene            complement(3160..3636)
                     /locus_tag="VFB52_01490"
     CDS             complement(3160..3636)
                     /locus_tag="VFB52_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05035.1"
                     /translation="MGILGRIKDGVVTKLDGPPKPPPHVWPDEQPWARLLSSERNPGA
                     IGDPNQDGAPSMLDDLIGKAAGVPYGFELEVHRPDRPTYTLARRQRVPSKVEGILFLR
                     SHSIPAGAEVPIRVSGPNPEDIELDWDAYLAIPDQSARAYHLRIEQSRAMQQRENG"
     gene            3737..4210
                     /locus_tag="VFB52_01495"
     CDS             3737..4210
                     /locus_tag="VFB52_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZO05036.1"
                     /translation="MAGGRAAGVVAALGIVLILGAATGAGCGSSDSGSSEAADYPWNQ
                     EFHGVEITEGDGGTPILVPADLGLSSEEQKGEDWIGWSINCNLFGADLEVDGDRMRVD
                     QVSSTAMGCPGEDEDEWLAEFLESGPEWRLQGEKLILTSGGDTVELAPGKVPSDG"
     gene            4258..4530
                     /locus_tag="VFB52_01500"
     CDS             4258..4530
                     /locus_tag="VFB52_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HZO05037.1"
                     /translation="MWRYELFKDEGGEWRWRLCGPEKQTVMTSGESFRSRGSARRALV
                     RARNYAGQATLPLADGLDDMFGEVLERIAKRDDEERTRRASIAALN"
     gene            4705..5484
                     /locus_tag="VFB52_01505"
     CDS             4705..5484
                     /locus_tag="VFB52_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05038.1"
                     /translation="MMVTAGLLAALVVASLAIAAPKPDNKPVKVIVGDLELEGNGIFK
                     PSALPRNKLAPISLLISGKVRSLDPNNAHPPALKELQVETDKNGTVEVKGYPVCPPSK
                     IQSTTTKAAKAACGKALIGQGKTTVEIEFPESSPVMVNSDLLMFNSGVRAGITTLLVH
                     AYITVPVPAAIVTTVKIKKINKGRYGLLSVASVPKIAGGSGSVTSFNLKIDKKYTYKG
                     KKLSVLNAKCPDGKLQAHATAIFADGTKASIEFARPCTPKG"
     gene            complement(5580..6041)
                     /locus_tag="VFB52_01510"
     CDS             complement(5580..6041)
                     /locus_tag="VFB52_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05039.1"
                     /translation="MDIREARPWFWILILVVFAVGAVGLVLAISAQNSSVDEDQVVKE
                     AKAEIKEELDGLNGAIEAADEFQEESDKQAAKERKRIETAVEEAEAGAQKRMRGLTNR
                     VKALEGETEDLAANDTKQAEQIEDLEADQETVSAELERINKRLRTITADGG"
     gene            6156..6389
                     /locus_tag="VFB52_01515"
     CDS             6156..6389
                     /locus_tag="VFB52_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05040.1"
                     /translation="MPTIIHFVGRDQLMVREDEEEVQAAFEAGEGRPIGLTHHRSGNR
                     VFVNPVVITHWAGRGSRSAIERRTEEARLPSLR"
     gene            complement(6386..7228)
                     /locus_tag="VFB52_01520"
     CDS             complement(6386..7228)
                     /locus_tag="VFB52_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05041.1"
                     /translation="MAKHTTRGGTEYDSADLDRVERLFWRDCWEGLDAGLAADHGVAL
                     RDFGPVQAQVVAELPAAGMLNQALGATLPGAIESGELGAAVEWMSARGGPPHVPVTPD
                     TPTTAGVEAWLTANGFEPGYAWMKFVRDPHPPRFREPSGVEVIEVDAPGQAPFGMIVA
                     TGFGAPPWAAGLFDLPGRAGWRCYVALIDDTPQACAAMRIDGGIAEFGMAATLEGARG
                     KGCQLALLRRRIVDAAAAGCHLLFVETGERIPDRPSISYRNILRAGFEEAYLRPNWKR
                     PIAD"
     gene            7254..>8413
                     /locus_tag="VFB52_01525"
     CDS             7254..>8413
                     /locus_tag="VFB52_01525"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZO05042.1"
                     /translation="MSVATASATAADSATEVPRDQVIVLFGATGDLAKRKLLPGMFHL
                     ARVGLMPERFRIVGAARNAISTEEFRELAREAVESSGRRPLEADDWDRFADSLRFVGV
                     GEGFEGLGEEVEAARAELAEDAGLLFYLSLPPRAAAGTVEQIGALGLGTGARVITEKP
                     FGTDLESAKALNAHLHAVFAEDCIFRIDHYIGREAVQNLLALRFANGMFEPVWNRNHI
                     DHVQIDIPETLSIEMRGSFYEETGAFRDMVVTHLFQVLGFVAMEPPVSLDPKPLGRER
                     EKVFDSMTPLSPDDVVRGQYDGYRGEEGVAPDSDTETFVAVRATVDNWRWEGVPFYLH
                     TGKRMAQKNHLLTIAFKQPPRRMFPLDCDQVVESFGHDHLTFELGDPGSISAS"
BASE COUNT         1395 a         2817 c         2853 g         1348 t
ORIGIN      
        1 aagaacagat caccgccttc gcaccgacgg cgttcaagct ggaaacgaaa ctgccggtca
       61 gtgtgaccac cggcgccggc attgccaccg gcgccgggac ctactcctac gtcggctgca
      121 aggtcccgac gctggcgaac aagaagctga agccggcgaa gcgggcgctg aagaaagccg
      181 gctgcaagct cggcaacgtg accctgcgct acggcgccac cgcgcggacc ggcaaagtgc
      241 tcagccagca cccgagtgcc ggcaccatcc ggctgccggg caccaaggtg aagctgaccc
      301 tctattaacc acgccgctag tcttgagcgg cttctcggcg ccttggcgga gtggctacgc
      361 agcggcctgc aaagccgttt acaccagttc gattctggta ggcgccttct cggtcgaact
      421 ccagcgggtt ggaccgccgg tcgggtcccg aatcgggagc ttggaccggc cggcaggggg
      481 ttgaaaaaaa tcaaggggtt cggtggtccg cgcgtccaag aaccggtccg cctggtttgt
      541 cccgaaggcc aaagacgctg cagtttttcg atcctaggtt ccttccttcc tggaaccgaa
      601 cggaaggcgc aggatctatg gctgatggtg agacggcggt tgctgagaag aaaggcgtcc
      661 agcccccgcc gacggagaaa gttcgcgacg acggcttctt gcccggcccg atggaccggc
      721 gctcgtttct cgtccgcacc ggtggggccg cggcggtggc tggagcggcc gcggccttcg
      781 gcccagcggc ttccgtgctg gctgcgccca agtctcctgc cgccagcacg gcggccagcc
      841 cgctgcccag cgcccaggca gtcctcgccg atggccgtga cctggtcgac cacgaagtcg
      901 tcgagctggc ggcgctgctg caagccggcg aggtctcctc ggtcgagctg acgcaggcct
      961 acctcgaccg catcaactcc ctcaacggcc cgtttgagac ctacgccgac aacaaccact
     1021 acaacggctt cgtccggatc gacgaggccg gcgccctggc cgccgcccga accgccgacg
     1081 aagctctagc cgccggtcgt cgcgatggca ccgcggtgcc gttcctgtgc gggatcccga
     1141 tgggcttcaa ggactcgatc gggaccgccg gcttcgccca acagaacgga accgtcgcct
     1201 atctcggcaa tgtcgccttc gaagacagca ccccggtcgc ctacctgcgc gccaagggcg
     1261 tcgtgccgct cgggatcacc gtctgctcgg ccttctccgg gacgatcgcc ggtaccttct
     1321 ccggcaacgc ctgggacctc gactacgtgc ctggcggatc gagccagggc tccggggtcg
     1381 ccacgatcgc acgcctcgcc gccgccaccc tcggcgagga gaccggcggc tcgatcatct
     1441 tccccgccgc ctgcaacggc gcctcggcga tcaagccctc ccagggcctg gtctcaaccg
     1501 ccggggtgat gccgctctcg ccgggctacg acacgatcgg cccgatctgc cgctccgggc
     1561 gcgactcggc gctgatcatg aacgcgctga tcggcgagga tcccttcggg gacccccaga
     1621 ccctcgccgt ccccgccggc ttcccgagcc tctcgctgac cccgcggatc ggctccaaac
     1681 cgctggccgg gatcacgatc gggatcaaca agaccgactg gctgacggtc gcggtcggcg
     1741 aaacgaccga aaccaaacgc aacgtcgacc cgcagtcgct ctacggggca ccgcatatcg
     1801 ccgccttcga acgcctgcgc ggggagctcg aggcgatggg ggcgaaagtc gtcgatttca
     1861 atggcgtcga cttcctcgaa ttcagcgagg aggccaatcc ctactacaac aaccccgacg
     1921 cgctgatgac gatcgaaggg gtgaacatca cccctgccaa cgccgtcgtc agccccaacc
     1981 gctacgacat ccgctacacc gaggcggtcc gggaattcgc cgaaacgcgc cctgccgggc
     2041 aggccacgga actgctcaaa cagtacggcc gccaggaaga ctgggacaaa gcgatcctcc
     2101 agcagtcggc gatcaccacc gcggtccgtc gcgagggcga gcgtcggcgg cgcaagtggg
     2161 ccgccaacta caaagcggcc ttcgacgccg ccggggtcga cttcatgctg gtgatgaccc
     2221 tgggcgacaa gccggcgttg cgagcggaca gtcgctatcc ctgccgccgc aacaatcagc
     2281 tcgccaacag catgagcttc ccgatggtca gcttcccgat cggctacgac ccggaggggc
     2341 tgccgatctg cgcccagttc aagggcccgc gcttctccga gccgcagctc gtccaggcga
     2401 tgatcgacta ccaggcacgg cactccgagc accacacggc acggccgcct gactacgccg
     2461 aaaaagcgag cttgaaactg tcgcgggcgc gctcgctggc gctcgagacc gaggacctct
     2521 cgctctcctc cgatccgctc gtcagcgagg aaaacttccg atgagcaaga tgacggtcga
     2581 ttgggaccgc ggcgagcaga tcgggttcat gcccgaggcc tccgaagtcg tctacccgga
     2641 gccgattctg acggtcgagt gggtgccgct gacgacggcg acgatcatcg actacgaccc
     2701 ggccgacctc ctggtcaccc cgggcgccgg cccagacgtt ccgcccggcg gcgggcccgg
     2761 cgagggcggc ggcccaggcg ggggtgacgg tccgggcgcc ccgacccact acggccggcc
     2821 gcagctggtg gtgcccaaac ggcgcagcga gaagtctttc ctcgagcacg gcctacggat
     2881 cgggatcgac gcgccggcac cggcgcgggt gcgagccgtg ctgaagggca agctgccgcg
     2941 aaccaagacc cagccatcgc gttggctgaa cctgacgaag gtggtgaccc aagacaccgg
     3001 cgcgggggag ggggcgctgg tcctggaccc ctcgcgcgcg ggccggcgcg ccctgcaccg
     3061 gctcgaagac tcggtccgtg ccgagctgat cctcgtcgtc cgctacgcca acggtcagga
     3121 tccgattcgg atcgagcgtg gattgacgat cgtcgatagc tagccgtttt cgcgctgctg
     3181 catcgcgcgg gactgctcga tccgcaggtg gtaggcgcgg gcgctttggt cggggatcgc
     3241 gaggtaggcg tcccaatcga gctcgatgtc ctcggggttg gggccgctga cgcggatcgg
     3301 gacctcggcg ccggcgggga tcgagtggct cctcaggaag aggatgccct cgaccttgct
     3361 cggtacgcgc tggcgacgcg cgagcgtgta ggtggggcgg tcggggcggt ggacctccag
     3421 ctcgaacccg tagggaacgc cggccgcctt cccgatcagg tcgtcgagca tcgacggggc
     3481 cccgtcttgg ttggggtcgc cgatcgcgcc ggggttgcgc tctgagctga gcaggcgggc
     3541 ccagggctgc tcgtctggcc acacgtgggg cggcggtttc ggcggcccgt cgagcttggt
     3601 gacaacgccg tccttgatcc ggccgagtat tcccacgctg gttcgcattc taggcttgga
     3661 ccttgcgatg ccgcgatcga agaactgcgc caagtggtca gcgagctttt gaggtcggca
     3721 gggtgttgat caggtgatgg ccggcgggag agctgcgggg gtggtcgcgg cgctggggat
     3781 cgtcctgatc ctcggcgccg cgaccggcgc cggctgcggc tcgtcggact cggggtcgag
     3841 cgaggctgcc gactatccgt ggaaccagga gttccacggg gtggagatca ccgaggggga
     3901 cgggggaacg ccgatcctgg tgccggctga cctcggcctc tcctccgagg agcaaaaggg
     3961 cgaggactgg atcggctggt cgatcaactg caacttgttc ggggctgatc tggaggtcga
     4021 cggggaccgg atgcgcgtcg accaggtcag ttcgacggcg atgggctgtc cgggggagga
     4081 cgaagacgag tggctggccg agttcctcga aagcggccct gaatggcgcc tgcagggcga
     4141 aaaactgatc ctcacgagtg gcggtgacac ggtcgagctg gcgcccggaa aagttccgtc
     4201 cgacggctag gcgaacatgt gttcgtatcc tagagagatc ggccaggagg gcgcgaaatg
     4261 tggagatacg agctgttcaa ggacgaaggc ggagagtggc gttggcggct ctgcggtccc
     4321 gaaaagcaaa ccgtgatgac ctcgggtgaa tcgttccggt cgcgggggag cgcgcgccgg
     4381 gcgctggtgc gggcgcggaa ctacgccggc caggcgaccc tgccgctggc ggatggtctc
     4441 gacgacatgt tcggcgaggt gctcgagcgg atcgccaagc gtgacgacga ggaacggact
     4501 cgccgggcgt cgatcgcggc gctcaactga tcgggacgga gtcgcaaggg gcggttccgt
     4561 aagggattcc taacgtacgg tttggtactt gcctggtagc ttccgcagac cacgaaggta
     4621 gtcaattgac gacgtgactc gcctcgggag ggcgagtcac aaagggggag agcagggatg
     4681 cggcttgtac gtagaagtgc gttcatgatg gtgaccgctg ggctgttggc ggcgcttgtc
     4741 gtcgcgagcc tggcgatcgc ggcgccgaaa ccggacaaca agccggtgaa agtcatcgtc
     4801 ggtgacctcg aactggaagg gaacgggatc ttcaagcctt cggctctgcc gagaaacaag
     4861 ctggcgccga tctcgttgct gatctctggc aaggtgcgct cgctcgaccc caacaacgcg
     4921 cacccaccgg cgctgaaaga acttcaggtc gaaaccgaca agaacggcac ggtcgaagtc
     4981 aagggttacc cggtctgccc gccgagcaaa atccagtcga cgaccacgaa agccgcgaaa
     5041 gctgcctgcg ggaaggcgct gatcggccaa ggcaagacca cggtggagat cgagttcccg
     5101 gaatcgtcac cggtgatggt caactcggac ttgttgatgt tcaacagcgg tgtccgcgcc
     5161 ggaatcacca cgctgctcgt gcacgcctac atcaccgttc cggtgccggc cgcgatcgtc
     5221 accacggtga aaatcaaaaa gatcaacaaa ggccgctacg gcctgctctc ggttgcctcg
     5281 gtcccgaaaa tcgccggtgg cagcggctcg gtgacctctt tcaacctcaa gatcgacaag
     5341 aaatacacct acaaaggcaa gaagctcagc gtcctcaacg cgaaatgccc tgacggcaag
     5401 ctccaggccc acgccacggc gatcttcgcc gacggcacca aggcctcgat cgaattcgcc
     5461 cgcccctgca cgcccaaggg ctagggcgct agctcgacgg cacgctcgcg acattgatcc
     5521 cgctctggtg tggtcaaagt cgcgagcgtg atcgtcagcg tcgtgaggcg cccgtccgct
     5581 cagccgccgt cggcggtgat cgtgcgcagg cgcttgttga tccgttccag ttcagccgag
     5641 acggtttcct gatcggcttc caggtcttcg atctgttccg cctgtttggt gtcgttggcg
     5701 gccaggtctt cggtttcgcc ttccagcgcc ttgacccggt tggtgaggcc gcgcatccgc
     5761 ttctgcgcgc cggcttcggc ttcttcgacc gccgtctcga tccgcttgcg ctctttggcg
     5821 gcctgtttgt cgctttcttc ctggaattcg tcggcggctt cgatcgcgcc gttgaggccg
     5881 tctagttctt ctttgatttc agctttcgcc tccttgacga cctggtcctc gtcgacgctg
     5941 ctgttctgtg ccgagatcgc cagcacaagg ccgacggcgc caaccgcgaa cacaacgagg
     6001 atgaggatcc agaaccaggg gcgagcttcc cggatatcca tggcggcgaa cgctatcgcg
     6061 ggcggcggcg gccgagctag gtgtacatcg cggttcgttt cccctatttg gggggttgac
     6121 agtcaggcct gggtgggacg tagtgttgcc cggcgatgcc gaccatcatt cacttcgtag
     6181 gccgtgacca gctgatggtc cgcgaggacg aggaggaagt gcaggccgcg ttcgaggccg
     6241 gcgaaggccg gccgattggc ctcacgcacc accgctcggg caaccgcgtc ttcgtcaacc
     6301 cggtggtaat cacgcattgg gccggccgcg gctcgcgcag cgcgatcgag cgccgcaccg
     6361 aggaagcgcg gctcccgagc ctgcgttagt cggcgatcgg gcgtttccag ttcggccgca
     6421 ggtaggcctc ttcgaagccg gcgcggagga tgttgcggta gctgatcgag ggccggtcgg
     6481 gaatccgctc gccggtctcg acgaagagca ggtggcaacc ggcggcggca gcgtcgacga
     6541 tgcgccgccg caacagcgcc agctggcagc ccttgccgcg ggctccctcg agcgtcgccg
     6601 ccattccgaa ctcggcgatg ccgccgtcga tccgcattgc cgcgcaggcc tgcggggtgt
     6661 cgtcgatcaa ggcgacgtag cagcgccagc ccgcgcggcc gggcaggtcg aagagcccgg
     6721 cggcccaggg cggggcgcca aacccggtcg cgacgatcat cccgaagggt gcctgccccg
     6781 gcgcgtcgac ctcgatcacc tcgaccccgc tgggctcgcg gaaacgcggc gggtgggggt
     6841 cgcggacgaa cttcatccag gcgtagccgg gttcgaaccc gtttgcggtc agccaggcct
     6901 cgaccccggc ggtggtcggc gtgtcgggag tcaccgggac gtgaggtggc ccgccgcgcg
     6961 cgctcatcca ctcgactgcc gcccccagct cgccgctctc gattgccccg ggcagcgtcg
     7021 ctccgagcgc ctggttcagc atgcccgccg ccggcagctc cgcgaccacc tgggcctgaa
     7081 ccggcccgaa gtcgcgcaac gcgactccgt ggtcggcggc gaggccggca tcgagcccct
     7141 cccagcagtc acgccagaac aggcgctcaa cccggtcgag gtcggcgctg tcgtactcgg
     7201 tgcccccgcg cgtggtgtgc ttggccatgc cggcctcact ctaagctgga gaggtgagcg
     7261 tcgccaccgc cagcgccacg gcagccgact cggcaaccga ggtcccgcgc gaccaggtga
     7321 tcgtcctctt cggggcgacc ggtgacctgg cgaaacgcaa gctgctgccc ggcatgttcc
     7381 acctcgcccg ggtcggcctg atgccggagc gcttccggat cgtcggcgcc gcccgcaacg
     7441 cgatctcgac cgaggagttc cgcgagctgg cgcgggaggc ggtcgagtcc tcgggtcggc
     7501 gcccgctcga ggccgacgac tgggatcggt tcgccgactc gctgcgcttc gtcggcgtcg
     7561 gcgagggctt cgagggactc ggcgaggagg tcgaggcggc gcgggcggag ctggccgagg
     7621 acgccggctt gctcttctac ctctcgttgc caccgcgcgc cgctgccgga acggtcgagc
     7681 agatcggggc gctggggctc ggcaccggcg cccgcgtgat caccgagaag ccgttcggta
     7741 ccgacctcga gtcggcgaag gcgctgaacg cgcacctgca cgcggtcttc gccgaggact
     7801 gcatcttccg gatcgaccac tacatcggtc gcgaggcggt ccagaacctg ctggcattgc
     7861 gcttcgccaa cgggatgttc gagccggtct ggaatcgcaa ccacatcgac cacgtccaga
     7921 tcgacattcc cgagacgctc tcgatcgaga tgcgcggcag cttctacgag gagacgggcg
     7981 cattccgcga catggtcgtc acccatctgt tccaggtgct cggcttcgtc gcgatggagc
     8041 cgccggtctc gctcgacccg aaaccgctcg gccgcgagcg cgagaaggtc ttcgactcga
     8101 tgacgccgct ctcgcccgac gacgtcgtcc gcgggcagta cgacgggtac cgcggcgagg
     8161 aaggggtcgc ccccgactcc gacaccgaga ccttcgtcgc ggtcagggcg acggtcgaca
     8221 actggcgctg ggagggggtg ccgttctacc tgcacaccgg caagcggatg gcgcagaaaa
     8281 accacctgct caccattgcc ttcaagcagc cgccgcggcg catgttcccg ctcgactgcg
     8341 accaggtggt cgagtccttc ggccacgacc atctcacctt cgagctcggc gaccccggca
     8401 gcatctcggc ctc
//
LOCUS       DASRIG010000056        10462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3677322, whole genome shotgun sequence.
ACCESSION   DASRIG010000056 DASRIG010000000
VERSION     DASRIG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677322"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1273
                     /gene="pstC"
                     /locus_tag="VFB52_01530"
     CDS             278..1273
                     /gene="pstC"
                     /locus_tag="VFB52_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZO05043.1"
                     /translation="MSAAESPGMPRRARPRRSSASRRKRFDRIGDATLRAVCILAVLL
                     AAAAVFGVVYEVIDGSNRAISEFGLGFVTDTAWQPNFEEFGAGSLIFGTIVSSFVALA
                     IGAPIAISIGLYLTLLAPAGVRSVVAPLVELLAAIPSVILGFWGIIVLAPFVRDHVEP
                     TLHDTLGFIPIFGEPQTTGLSVFTASLILTIMILPIIASISRDLFQAVPRDVQDGASA
                     LGATRWEVVRGVVLPSTASGLVAASLLGLGRALGEAIAVAQVIGAGSEIHASLFETGD
                     TLAARIANEFPGALTELHQSALFYCGLILLVIGLISNLLAQWVGRRFGYQGAAAK"
     gene            1270..2154
                     /gene="pstA"
                     /locus_tag="VFB52_01535"
     CDS             1270..2154
                     /gene="pstA"
                     /locus_tag="VFB52_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZO05044.1"
                     /translation="MRGRGAIPDPSAPLTPSGNLRRRQVVSKFAEYAATAAAALAVAV
                     LAIVIYTVASRGADALSFDFLVKGAPDGIGPMLVGTGVIVGVATLMAAPVGILIALYV
                     TEYAGRRSARVIQMTLDVMNGLPSIIIALFIFGLLVYKKEQSGFAGSIALAIIMVPLI
                     ARGTQEILLLVPKAQREAADALGVARWRTVLTIILPSAIGGIATATVLAVARAAGETA
                     PLLFASSNFNPSEYALNPFDPLPNVPIRIFQLSEDPNPEAVTEAWGLALVLVTLILIA
                     SLSARFLLARSKRKMGGT"
     gene            2151..3062
                     /gene="pstB"
                     /locus_tag="VFB52_01540"
     CDS             2151..3062
                     /gene="pstB"
                     /locus_tag="VFB52_01540"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZO05045.1"
                     /translation="MSAHDNGFGAGVGPGGPAPRVRLRSEPLPQTADAAVAVEGHRTP
                     LTEAVFDAQDVAVFYGQKEALTEVSLKIRRGQITALIGPSGCGKTTFLRSLNRMNDSV
                     AGFRIGGKILYHGHDMYAGNVDQVEVRRRIGMVFQKPNPFPKSIYDNIAWAPRNLGLR
                     SGLDERVEKALRGAALWDEVKDRLKDSAFGLSGGQQQRLCIARAIAIEPDVLLLDEPA
                     SALDPIATAAIEGLMHSLKGRFTIVIVTHNMQQAARVADRTAFFSLQSSDGAKPVGTL
                     IEFDETEAIFSQPRDPRTRDYVSGRFG"
     gene            complement(3288..3770)
                     /locus_tag="VFB52_01545"
     CDS             complement(3288..3770)
                     /locus_tag="VFB52_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05046.1"
                     /translation="MSWREKLSFRGPRRTGTPSKAFTPAANGNGVAPPTTGTTPAAPA
                     GPAQAPGADESSVDLRRRRDQLMARVAELQWDLGGLAYEMAIRDRMQIELLVRRAVAL
                     QDADAELSEVERIVRMEETGTAGSCGNCGAPHSSGATFCWQCGKPLLRQISGDSILRS
                     "
     gene            complement(3767..4492)
                     /locus_tag="VFB52_01550"
     CDS             complement(3767..4492)
                     /locus_tag="VFB52_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05047.1"
                     /translation="MSVSPDPSEGGTPELPQGPSGEQPTQPLPPVPPQPGPPVPPAAA
                     SPSSSDLCPNCHARMAPDQRYCLSCGNRRGEPRLPFMDAVTFMDSMHGPPPSAAAAPP
                     PSPPRQRFSSGTTLVAGVATLVLAIGVGVLIGQSGDEGSQPVASQAPIVVKTEGGGGG
                     STETEGEAADGKGKNGGGKDKGGKGEIDTGSSGTSEGAEEFIKPKSGVKLAAPETKVG
                     EKCDKDVAGCSDSGKFDGSFFGE"
     gene            4623..6245
                     /locus_tag="VFB52_01555"
     CDS             4623..6245
                     /locus_tag="VFB52_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO05048.1"
                     /translation="METCSSCKAPLAVDQRFCVECGSRRSGLPAAVAAVVRNFASPPA
                     PVAAPPPVTPKSSMFGTWIPSPRSAAVAVLAMIALGVILGSVGQKFAQSAGLAPILVE
                     VPPSESAPPPVEEEVEEEPEEEAAPEEVVPEEEVVPEEAEEEVPETEPPPEEKPELPP
                     EEPPLPPVKHVFVIVLGENSYEETFGETSTAPYLAEQLPAAGQLLPNYFAVAGSGLAN
                     QIALLSGQGPTPETAANCPLYSDLAPGTVSEETGQAEGSGCVYPAAVETLADQLVEKE
                     LTWKAYVGDIGNNPAEPASCRHPPLGAADPRQVAVAGDAYVTWRNPFVYFHSILDGPE
                     CAELDVGLDRLTADLEAEETTPTLSYIVPSTCQDGGPVPCVPGGQAGPLEAEALLSTI
                     VPQIIASQAYKSGGLIAITSSQAQQGGTAPDASGCCVFPAYPNVPPAPEEKIVDSTRR
                     SGGGGKVGLLLISPYVEPGTVNEAYYNHYSLLRSIEEVLELPTLGYAAEPALTGFDNA
                     VYGLAPTAEEEEWLLPRPALNRRAADPASPSR"
     gene            complement(6205..8349)
                     /locus_tag="VFB52_01560"
     CDS             complement(6205..8349)
                     /locus_tag="VFB52_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05049.1"
                     /translation="MFDALARLADGNARRIGIFAIVLFVLAGAIGGGVADRLDPYGAD
                     DPSTETVKAEQRLQDAGLRSPAVIAVVPDAPVSEPATRARVTGLEDEVRERSDVAAVS
                     GYYTTRSPAFVSADGDSTYFAISLKPTGDKEVQDAGAEIADELTGSDPDVVVGGYAVA
                     QEQVNKQVEEDLKKAEMLAFPLLFLLSLLFFRSLVASLLPLMIGALAIVGTFLILRVA
                     SEFGSISVFALNLTTALGLGLAIDYSLFIVSRYREEIAKTGPGLAAMKRVLATAGRTV
                     FFSSLTVAAALASLLVFPQRFLYSMGLGGALVALFAALISLTVLPAVLTLLGNRVNAG
                     APKFLQRRAEADARPDSQGFWYRLSRFVQRRPIPVATASALLLIVMGLPFLGIKFNTV
                     DPTVLPESASARQAYDTISEEFPPYHDTPIWVAFEGGGPKDAAAFAAEVRGTEGAAEV
                     LPPQPLGDGVTAIQVISANPFASNASQDTVRTIRDLPTPAGTTALVTGASADFIDFQS
                     SLTSHLPIALAIVIVATLVILFLFTGSVVLPIKSLLMNFLNLSAVFGLLVLIFQDGRL
                     EGFLDYSSPGAIEQTMPILLFAVAFGLSTDYAVFLLSRIKEARDNGASDNEAVAIGLE
                     RTGRIVTAAAVLFAVALGAFATSQIIFIKENGVGTALAVLIDATIIRALLVPSLMELL
                     GKWNWWAPAPLRRLHERWGISETATPDPLPAD"
     gene            complement(8354..8926)
                     /locus_tag="VFB52_01565"
     CDS             complement(8354..8926)
                     /locus_tag="VFB52_01565"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZO05050.1"
                     /translation="METLSPTAYVILGLVRHEPRSGYEIKAIVDNTTRFFWAASYGQI
                     YPELKRLSEAGLVVGIDSPTGGRRRTVYEITADGEEELIAWLRQPPVTFEMREEGLLK
                     LFFAGALPREDAVEILRAMRAHRLAVNAQLRTMEQGAAEKDDPYTLMVLRSGLEFTEW
                     FAGWCERMEQELLAAPEATPNEKTPTENKE"
     gene            9074..9307
                     /locus_tag="VFB52_01570"
     CDS             9074..9307
                     /locus_tag="VFB52_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05051.1"
                     /translation="MGLDQSKIGRVVAEQMEAIEGDYGEDCEIGDVCTIVEVIGPHGS
                     HVRVRSSDMRPHSGLGLIRMAEQAMLGNLGGTE"
     gene            9396..>10462
                     /locus_tag="VFB52_01575"
     CDS             9396..>10462
                     /locus_tag="VFB52_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZO05052.1"
                     /translation="MSRKFVLLAALAAALTALLSSTASAFTFAPAQIRVAGLAQQEMH
                     GVEAADIDGDGLLDAVASGYYSGAPGGDPVIAVLRGRANGTLAPVVNYPMTGAGPVTG
                     LALGDFNEDGSPDAALGSPFSEELVISLNDGDGGFEAPTPLPSGGFSPDDPLVADFDA
                     DGHLDLASANGSSYSVYLGNGDGSFEPALNEEIEDWALTIAAGDFDDDGIVDLALGNV
                     DAGTVRILLGEGDGTFVAAPPVDLEALAPEEECFCVEIWGIAAGDLDGNGRDDLVVSD
                     RFENRLFSVLSNPDGTFTGAPPVDLEELAPEEECFCVEIWGIAAGDIDGNGTVDADDL
                     TVFANNFGRRSGATQVDGDID"
BASE COUNT         1624 a         3638 c         3594 g         1606 t
ORIGIN      
        1 cgagggcggc gatggcgagt ggcgtgcgca gcaacctatt acgagtcaaa acagtccctt
       61 cactcgagtt cgaagcaggt cattgaagtc gtcgcacggc gccggaccgg acggccccgt
      121 tgcggcgata gcgtctcgcc cgtccggggc ggtggtgttg cagacctgtg aaaagaacgt
      181 gtctgtgtga ggtcacattc gtgaccttgt ttcaatcctg tgaaaatcgc tcgcggcggg
      241 catatggagc gggtcctttc gcttaccctg ccgcgcgatg agcgccgccg aaagccccgg
      301 tatgccccgc cgcgcccgtc cccggcgaag ctcggcctcg cggcgcaaac ggttcgaccg
      361 gatcggcgac gcgaccctgc gcgcggtctg catcctcgcc gtgctgctcg ccgccgcggc
      421 ggtcttcggc gttgtctacg aggtgatcga cggctcgaac cgggcgatct cggagttcgg
      481 cctcggcttc gtcaccgaca ccgcctggca gcccaacttc gaagaattcg gggccggctc
      541 gctgatcttc ggcacgatcg tcagctcctt tgtcgcgctc gcgatcggcg ccccgatcgc
      601 gatctcgatc ggcctctacc tgaccctgct ggcgccggcg ggcgtgcgca gcgtcgtcgc
      661 ccctctggtt gagctgctcg ccgcgatccc cagcgtgatc ctcggcttct gggggatcat
      721 cgtgctcgcc cccttcgtcc gcgaccacgt cgagcccacc ctccacgaca cgctcggctt
      781 catcccgatc ttcggcgaac cgcagacgac cggcttgagt gtcttcaccg ccagcctgat
      841 cctgacgatc atgatcctgc cgatcatcgc ctcgatctcg cgcgatctct tccaggccgt
      901 gccgcgggac gtccaggacg gcgccagcgc gctcggcgcg acccgctggg aggtggtgcg
      961 tggggtggtg ctgccctcga ccgcctccgg tctcgtcgcc gcttcgctgc tcggcctcgg
     1021 ccgcgcgctc ggtgaggcga tcgccgtcgc ccaggtgatc ggcgccggct cggagatcca
     1081 cgcttcgctg ttcgaaaccg gcgacacgct ggcggcgcgg atcgccaacg aattccccgg
     1141 tgcgctcacc gaactgcacc agtccgcgct cttctactgc ggcctgatcc tgctggtgat
     1201 cggcctgatc tcgaacctgc tcgcgcagtg ggtcggtcgc cgcttcggct accagggggc
     1261 ggcggcgaag tgaggggccg cggcgcgatc cccgatccgt cggcgccgct gacgccgagc
     1321 ggcaacctgc gccgccgcca agtggtcagc aagttcgccg agtacgcggc gacggcggcg
     1381 gcggcgctcg cggtcgcggt gctggcgatc gtgatctaca ccgtggccag ccgcggcgcc
     1441 gatgcgctca gcttcgactt cctggtcaag ggggcgcccg acgggatcgg gccgatgctg
     1501 gtcgggaccg gcgtgatcgt cggggtcgcg acgctgatgg cggcgccggt cgggatcctg
     1561 atcgcgcttt acgtcaccga gtacgcgggg cgtcgctccg ctcgcgtgat ccagatgacg
     1621 ctggacgtga tgaacgggct gccctcgatc atcatcgccc tgttcatctt cggcctgctc
     1681 gtctacaaaa aggagcagtc ggggttcgcc ggttcgatcg cgctggcgat catcatggtc
     1741 ccgctgatcg cgcgcggcac ccaggagatc ctcctgctgg taccgaaagc gcaacgcgaa
     1801 gcggccgacg cgctgggagt ggcccgctgg cgaaccgtgc tgacgatcat cctgccgagc
     1861 gcgatcggcg ggatcgccac cgcgaccgtg ctcgcggtcg cccgcgccgc cggcgagacc
     1921 gcgccgctgc tctttgccag ctcgaacttc aacccgagcg agtacgcgct gaaccccttc
     1981 gatccgctgc ccaacgtccc gatccggatc ttccagctct ccgaagaccc gaaccccgaa
     2041 gcggtcaccg aagcctgggg gctggcgctg gtcctcgtca ccctgatcct gatcgccagt
     2101 ctcagcgccc gcttcctgct ggcccgcagc aaacgcaaga tgggggggac gtgagcgcgc
     2161 acgacaacgg gttcggggcc ggggtggggc caggcgggcc ggcgccgcgg gtgcgcctgc
     2221 gcagcgagcc gctgccgcag accgccgacg cggccgtcgc cgtcgaaggc caccgcacgc
     2281 cgctgaccga agccgtcttc gacgcccagg acgttgccgt cttctacggc cagaaggaag
     2341 cgctgaccga ggtctccctg aagatccggc gcggccagat caccgccctg atcggccctt
     2401 ccggctgcgg caagacgacc ttcctgcgca gcctcaaccg gatgaacgac tcggttgccg
     2461 gcttccgcat cggcggcaag atcctctacc acgggcacga catgtacgcc ggcaacgtcg
     2521 accaggtcga ggtgcgccgg cggatcggca tggtcttcca gaaaccgaac ccgttcccga
     2581 agtcgatcta cgacaacatc gcctgggcgc cacgcaacct cggcctgcgt tccgggctcg
     2641 acgagcgggt cgagaaagcg ctgcgcgggg cggcgctctg ggacgaggtc aaagaccgcc
     2701 tcaaggacag cgccttcggc ctctccggcg gccagcagca gcgcctctgc atcgcccgcg
     2761 cgatcgcgat cgagcccgac gtgctcctgc tcgacgagcc cgcctcggcg ctcgacccga
     2821 tcgccacggc ggcgatcgag ggtttgatgc acagcctcaa aggccgcttc acgatcgtga
     2881 tcgtcaccca caacatgcag caggcggccc gggtcgccga ccggaccgcg ttcttcagcc
     2941 tgcagtcgtc agacggcgcg aagccggtcg gcacgctgat cgagttcgac gagaccgagg
     3001 cgatcttctc ccagccgcgc gacccacgca cccgcgacta cgtcagcggc cgcttcggct
     3061 agcgccccat accgcgcgat ccaacggccc cggccaggag cctccgccac ggcccgccag
     3121 ccagacggaa cccccgggat ggcccgtgag gaggggtcgg gtgactcccg gaacgtcttg
     3181 tactttttcg ccgcccccaa tctcggacgg ctctatcccg atctcgaaaa agttaagtga
     3241 agggagtcac ccgacccctc cccgcatgtc gccccgaggt acgcccgtca gctacgcagg
     3301 atcgagtcgc ccgagatctg gcgcagcagc ggtttgccgc attgccagca gaaggtggcg
     3361 ccgctgctgt ggggggcgcc acagttgccg caggagccgg cggtgccggt ctcctccatg
     3421 cggacgatcc gctcgacctc gctcagttcc gcgtcggcgt cctgcagggc gaccgcgcgg
     3481 cggaccagca gctcgatctg catgcggtcc cggatcgcca tctcgtaggc gagcccgccg
     3541 aggtcccact gcagctcggc gacccgggcc atcagctggt cgcgacggcg cctcaggtcg
     3601 accgaggact cgtccgcccc gggcgcttgg gcgggaccgg ccggagctgc cggggtcgtc
     3661 ccggttgtgg gtggcgcgac gccgttgccg ttggcggccg gcgtgaacgc tttgctcggc
     3721 gtcccggtcc ggcggggccc gcggaagctc agtttctcgc gccagctcat tcgccgaaga
     3781 agctcccgtc gaacttgccg ctgtcactgc agccggcgac gtccttgtcg catttttccc
     3841 cgaccttggt ttcgggtgct gcgagtttca caccgctttt gggtttgatg aattcttcgg
     3901 ccccctcgga ggtgccgctg ctgccggtgt cgatctcgcc tttcccgcct ttgtctttgc
     3961 cgcccccgtt cttgcctttg ccatccgcgg cttcgccttc ggtttcggtg gacccgccgc
     4021 caccgccttc ggttttgacc acgatcggcg cctggctcgc gaccggctgc gagccctcgt
     4081 cgcccgactg gccgatcagc acgccgacgc cgatcgcgag gaccagcgtg gcgacgccgg
     4141 cgaccagggt ggtgccggag gagaagcgct ggcgcggtgg cgagggcgga ggtgcggccg
     4201 ccgcgctcgg gggcgggccg tgcatcgagt ccatgaaggt gaccgcgtcc atgaacggca
     4261 gccgcggctc gccgcggcgg ttgccgcacg agaggcagta gcgctggtcg ggcgccatcc
     4321 gcgcgtggca gttggggcag aggtcgctgc tggagggtga agctgcagcc ggaggcaccg
     4381 gcggtcccgg ttgcggtggc actggcggca gcggctgcgt cggctgctcg ccgctcggtc
     4441 cctgcggcag ctccggcgtg cccccctcgc tcggatcggg actcacactc acctgggcat
     4501 cttcagagtc accacccgcc ccaggtgttt aagggttgta aaagcccgga cacacttggt
     4561 tagcgttcgg gattcaggat cttcacaccc tggtaacggg gccgaccgat actcccggcc
     4621 acgtggagac ctgttcctcc tgcaaggcgc cgctcgccgt cgaccagcgc ttctgcgtcg
     4681 agtgtggctc ccgtcgcagc ggcctgccgg cggcggtcgc cgcggtcgta cgcaacttcg
     4741 cctctccgcc ggccccggtg gcggccccgc cgccggtgac gccgaagagc tcgatgttcg
     4801 ggacctggat cccgtcgccg cgctcggccg ccgtggcggt gctggcgatg atcgcgctgg
     4861 gcgtgatcct cggctcggtc ggccagaagt tcgcgcagag cgccgggctg gcgccgatct
     4921 tggtcgaagt gccgccttcg gaatcggctc caccgccggt cgaagaagaa gtggaagaag
     4981 aacccgaaga agaagcggcg cccgaagaag tggtgccgga agaagaagtg gtgccggaag
     5041 aagccgaaga agaagtcccg gaaacggaac ccccgccgga agaaaagccg gagctgccgc
     5101 ccgaagaacc gccgctgccc ccggtcaagc acgtcttcgt gatcgtgctc ggcgaaaaca
     5161 gctacgagga aaccttcggc gagacctcga ccgcgcccta cctggccgaa cagctccccg
     5221 ccgccggcca gcttctgccc aactacttcg cggtcgcggg cagcggcctc gccaaccaga
     5281 tcgccttgct cagcggccag ggcccgacgc ccgagaccgc cgccaactgc ccgctctact
     5341 ccgacctggc gccgggcact gtctccgagg aaaccggaca ggcggaaggg agcggttgcg
     5401 tctatccggc ggcggtcgaa acgcttgctg accagctggt cgagaaggag ctgacctgga
     5461 aggcctacgt cggcgacatc ggcaacaacc ccgcggaacc ggcgagctgt cgccacccac
     5521 cgctcggcgc cgccgacccg cgccaggtcg cggtcgccgg ggacgcctac gtgacctggc
     5581 gcaacccgtt cgtctacttc cactcgatcc tcgacgggcc cgaatgcgcc gaactcgacg
     5641 tcgggctcga ccggctgacc gccgacctcg aagcggaaga gacgacgccg accctctcct
     5701 acatcgtccc tagcacctgc caggacggcg gcccggtgcc ctgcgtgccg ggcgggcagg
     5761 cgggaccgct ggaggcggaa gcgcttctga gcacgatcgt gccccagatc atcgcctcgc
     5821 aggcctacaa aagcggcggc ctgatcgcga tcacctcgag ccaggcccaa caggggggaa
     5881 cggcgcccga tgccagtggc tgctgcgtct tccccgcata cccgaacgtg ccgccggcgc
     5941 ccgaagaaaa gatcgtcgac tcgacgcgcc gcagcggcgg gggcgggaaa gtcgggttgc
     6001 tgctgatctc tccctacgtc gagcccggca ccgtcaacga ggcctactac aaccactact
     6061 cgctgctgcg ctcgatcgag gaagtgctcg aactgccgac gctcggttac gccgccgaac
     6121 cggcgctgac cgggttcgac aacgccgtct acggactggc gccgacggcc gaagaagaag
     6181 aatggctgct gccgcggccc gcgctcaatc ggcgggcagc ggatccggcg tcgccgtctc
     6241 gctgatcccc caacgttcgt gcaggcggcg cagcggcgcc ggtgcccacc agttccactt
     6301 gccgagcagc tccatcagcg agggcacgag cagggcgcgg atgatcgtcg cgtcgatcag
     6361 caccgccaag gccgtgccga cgccgttctc cttgatgaag atgatctggg aggtggcgaa
     6421 ggcgcccagc gcgaccgcga agaggaccgc ggccgcggtg acgatgcggc cggtgcgctc
     6481 gagcccgatc gccaccgcct cgttgtcgga ggcaccgttg tcgcgcgcct ccttgatccg
     6541 cgagagcagg aacacggcgt agtcggtgga gaggccgaag gcgaccgcga acagcaggat
     6601 cggcatcgtc tgctcgatcg cgccgggact gctgtagtcg aggaagccct ccaggcggcc
     6661 gtcttggaag atcagcacca gcaggccgaa gaccgcgctg aggttgagga agttcatcag
     6721 cagcgacttg atcggcagca ccaccgagcc ggtgaacagg aagaggatca ccagggtggc
     6781 gacgatcacg atcgcgagcg cgatcggcag gtggctggtc aggcttgact ggaagtcgat
     6841 gaagtcggcg ctggcgccgg tgaccagcgc agtcgtgccg gccggggtgg ggaggtcgcg
     6901 gatcgtccgc accgtgtcct ggctggcgtt ggaggcgaac gggttggcgg agatcacctg
     6961 gatcgcggtg acgccgtcgc cgagcggctg cggcggcagc acttcggcgg cgccctcggt
     7021 tccgcgcacc tcggccgcga aggcggccgc gtccttcggc ccgccgcctt cgaaggcaac
     7081 ccagatcggg gtgtcgtggt agggcgggaa ttcctcggag atggtgtcgt aggcctggcg
     7141 ggcgctcgcc gactccggca gcacggtcgg gtcgacggtg ttgaacttga tcccgaggaa
     7201 gggcaggccc atcacgatca gcagcagcgc actcgccgtc gccaccggga tcggccgccg
     7261 ctgcacgaag cgcgagagcc ggtaccagaa gccctggctg tcgggccggg cgtcggcctc
     7321 ggcgcggcgc tgcaggaact tcggcgcgcc ggcgttgacc cggttgccga gcagggtcag
     7381 caccgccggc agaacggtca gcgagatcag cgccgcgaac agcgcgacca gcgcgccgcc
     7441 caggcccatc gagtagagga agcgctgggg gaagacgagc agcgaggcca gcgccgcggc
     7501 gacggtcagc gaggagaaga agacggtgcg cccggcggtc gccagcaccc gcttcatcgc
     7561 cgccagcccc gggccggtct tggcgatctc ctcgcgatag cgggagacga tgaagaggct
     7621 gtagtcgatc gccagcccga gccctaacgc cgtggtcagg ttgagcgcga agaccgagat
     7681 cgagccgaat tcggaggcga cccggaggat caggaaggtg ccgacgatcg ccagggcgcc
     7741 gatcatcagc ggcagcagcg aggcgacgag gctgcggaag aagagcagcg agagcaggaa
     7801 gagcagcggg aaggcgagca tctccgcctt tttcaggtct tcctcgacct gcttgttgac
     7861 ctgctcctgg gcgaccgcgt agccgccgac gacgacgtcg gggtcagagc cggtgagctc
     7921 gtcggcgatt tcggcgccgg cgtcctggac ctctttgtcg ccggtcggct tcagcgagat
     7981 cgcgaagtag gtggagtcac cgtccgcgga gacgaaagcg ggggagcggg tcgtgtagta
     8041 gccgctgacc gcggcgacgt cggagcgttc gcggacctcg tcttcgaggc cggtgacccg
     8101 ggcgcgggtc gcgggctcgc tgaccggcgc gtcggggacg acggcgatca ccgcgggcga
     8161 gcgcaggccg gcgtcctgca gccgttgctc cgccttcacg gtctcggtcg agggatcgtc
     8221 ggccccgtag gggtcgaggc gatcggcgac gccgccgccg atcgcgccgg cgagcacgaa
     8281 gaggacgatc gcgaagatgc cgatccggcg ggcgttgccg tcggcgagtc gggcgagggc
     8341 gtcgaacatg gccttactcc ttgttctcgg tgggggtctt ctcgttgggg gtcgcctcgg
     8401 gcgcggcgag cagctcctgc tccatgcgct cgcaccagcc ggcgaaccac tcggtgaact
     8461 cgagcccgct gcgcagcacc atcagcgtgt acgggtcgtc cttttcggcg gcgccctgtt
     8521 ccatcgtgcg cagctgggcg ttgaccgcga gccggtgggc gcgcatcgcc cgcaggatct
     8581 cgaccgcgtc ctcgcgcggc agggcgccgg cgaagaacag tttcagcagg ccttcctcgc
     8641 gcatctcgaa agtcaccggg ggctggcgca gccaggcgat cagctcctcc tcgccgtcgg
     8701 cggtgatctc gtagaccgtg cgccgccggc cgccggtcgg cgagtcgatc ccgaccacca
     8761 gccccgcctc agagagccgt ttcagctccg ggtagatctg cccgtagctg gcggcccaga
     8821 agaagcgggt cgtgttgtcg acgatcgcct tgatctcgta acccgagcgc ggctcgtgcc
     8881 gaaccaggcc gaggatcacg taggcggtgg ggctgagcgt ctccatcgcg ggtagatata
     8941 tcgctccgat atatccttaa gtgtgattac gatcacacga gatatccgat ccaaaaccgc
     9001 ccaccgctgc ggcccgacta aggtggggaa aacgtcggct gcgccgacca ggcggtgtgg
     9061 aaaggggatt gcgatggggc ttgaccagag caagatcggt cgggtcgtgg ccgagcagat
     9121 ggaggcgatc gaaggggact acggcgagga ctgcgagatc ggcgacgtct gcacgatcgt
     9181 cgaggtgatc ggcccgcacg gctcccacgt ccgcgtgcgc agcagcgaca tgcgcccgca
     9241 cagcggcctc ggcctgatcc gcatggccga acaggcgatg ctcggcaacc tgggcggcac
     9301 cgagtagtcc tcgaacaacg gtccgactcc agggtggtgt ccgagcggcg cgcctagact
     9361 taccgaacac cgtccggtaa gtcgaaaggg agtccatgtc tcggaagttc gtgctgctgg
     9421 ccgcgctcgc ggccgcgctc accgccctgc tgtcgtccac cgcctccgcc ttcaccttcg
     9481 cgcccgccca gatccgggtc gccggcctgg cgcagcagga aatgcacggg gtggaggccg
     9541 ccgacatcga cggcgacggg ctgctcgacg cggtcgcctc cggctactac tcgggggcgc
     9601 ccgggggaga cccggtgatt gccgtcctgc gcggccgcgc caacggaacc ctggcgccgg
     9661 tggtgaacta tccgatgacc ggcgccggtc cggtgaccgg gctcgcgctc ggcgacttca
     9721 acgaggacgg cagccctgac gcggcgctgg gctcgccctt cagcgaagaa ctcgtgatca
     9781 gcctcaacga cggcgacggc ggcttcgaag cgccgacccc cttgccctcc gggggcttca
     9841 gccccgacga cccgctggtc gccgacttcg acgccgacgg ccacctcgac ctcgcctcgg
     9901 ccaacggcag ctcctactcg gtctacctcg gtaacggcga cggcagcttc gaaccggcgt
     9961 tgaacgagga aatcgaagac tgggcgctga ccatcgccgc cggcgacttc gacgacgacg
    10021 ggatcgtcga cctcgcgctc ggcaacgtcg acgccggcac ggtgcggatc ctgctcgggg
    10081 aaggcgacgg caccttcgtc gcagccccgc ccgtcgacct ggaagcgctt gcccccgaag
    10141 aggaatgctt ctgcgtcgaa atctggggga tcgccgccgg tgacctcgac ggcaacggcc
    10201 gcgacgacct cgtggtcagc gaccggttcg aaaaccgcct cttctccgtg ctctccaacc
    10261 ccgatggcac cttcaccggg gcgccgcccg tcgacctcga agagctcgcc cctgaagagg
    10321 aatgcttctg cgtcgaaatc tgggggatcg ccgccggcga catcgacggc aacggcaccg
    10381 tagacgccga cgacttgacc gtgtttgcaa acaactttgg ccgcaggagc ggcgcgacgc
    10441 aggtcgatgg cgacatcgac tt
//
LOCUS       DASRIG010000057         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3419980, whole genome shotgun sequence.
ACCESSION   DASRIG010000057 DASRIG010000000
VERSION     DASRIG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419980"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFB52_01580"
     CDS             complement(<1..227)
                     /locus_tag="VFB52_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZO05053.1"
                     /translation="MTAGVKRVALAYSGGLDTSIIIPWLKEHYHCEVVAIAADVGQGE
                     ELEGLREKALATGAAEIYIEDLRHEFVTEYI"
     gene            complement(352..1509)
                     /gene="moeB"
                     /locus_tag="VFB52_01585"
     CDS             complement(352..1509)
                     /gene="moeB"
                     /locus_tag="VFB52_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZO05054.1"
                     /translation="MPELTKEEVARYSRHLIMPEVGMEGQRKLKAASVLLIGTGGLGS
                     PLGLYLAAAGVGRLGLVDFDVVDHSNLQRQVLHGTRDVGRPKIDSARDRIADLNPHVQ
                     IDAHETALKAENAFAILKGYDVIIDGTDNFPTRYLTNDASVLLGKPNVYGSIFRFDGQ
                     ASVFYPKAGGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKLIIGEG
                     EPLVGRLLLFDALKMKFRELKLRRNPECPLCGDHPTIKELIDYEQFCGITPEPAEAPG
                     ASDWEITPAELKAKLDRGEEVTIIDVREPHEWQIANLENYGSKLIPLGQFPARLNELN
                     SAEEIVVHCKMGGRSAKAYEALKQAGFKKIKNLKGGITAWADQVDRSMPKY"
     gene            complement(1555..1812)
                     /locus_tag="VFB52_01590"
                     /pseudo
     CDS             complement(1555..1812)
                     /locus_tag="VFB52_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            complement(1834..2271)
                     /locus_tag="VFB52_01595"
     CDS             complement(1834..2271)
                     /locus_tag="VFB52_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZO05055.1"
                     /translation="MGINLTGEHLEAIKRHGEATYPEECGGLLLGTLEDGVRVIREVL
                     PLANVRPDSRHNRVELDPRDYLKAEREAGRRGLGVWGYYHSHPDHPAEPSGFDLAHAP
                     FTEWAYLIVSVRGGLSAEVRAWSMRHDRSQFDEEELIQIEETA"
     gene            complement(2277..>2467)
                     /locus_tag="VFB52_01600"
     CDS             complement(2277..>2467)
                     /locus_tag="VFB52_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05056.1"
                     /translation="GISAAANVAAALRLASRLEEGVIVTILCDDGSKYLSENFWEEGD
                     QPSAVSGQPEGGPGLAES"
BASE COUNT          370 a          832 c          813 g          452 t
ORIGIN      
        1 aagatgtact cggtgacgaa ctcgtggcgc aggtcctcga tgtagatctc ggccgccccc
       61 gtggcgagcg ccttctcacg cagcccctcg agctcctccc cctggccgac gtcggccgcg
      121 atcgcgacca cctcgcagtg gtagtgctcc ttgagccacg ggatgatgat cgaggtgtcg
      181 agcccgcccg agtaggcgag cgccacccgc ttgacgcctg ctgtcataga caatgaagcc
      241 tcctgaaaat agatttcagg gattatacac gccgggcggg ggagaggcca cggatgaggc
      301 gagccgaacg ggtcggcgcg gatttcccaa cccgcgccga cccgcgggcg atcaatactt
      361 gggcatcgac cggtcgacct ggtcggccca ggccgtgatc ccgcccttga ggttcttgat
      421 ctttttaaac ccggcctgct tgagcgcctc gtaggccttc gcgctccggc cgcccatctt
      481 gcagtggacg acgatctcct cggcgctgtt gagctcgttg agccgtgcgg ggaactgccc
      541 gagcgggatg agcttcgacc cgtagttctc gaggttggcg atctgccact cgtgcggctc
      601 gcgcacgtcg atgatcgtca cctcttcccc gcggtcgagc ttggccttga gctccgccgg
      661 ggtgatctcc cagtcagaag cgccgggcgc ctcagccggc tcgggcgtga tcccgcagaa
      721 ctgctcgtag tcgatgagtt ccttgatcgt cgggtggtcg ccgcagagcg ggcactcggg
      781 gttgcgccgc agcttcagct cgcggaactt catcttaagc gcgtcgaaga gcagcagccg
      841 gccgacgagc ggctcgccct cgccgatgat cagcttgatc gcctcggtcg cctggatgat
      901 cccgatgatc cccggcagga tgccgaggac gccgccctcg gcgcacgacg ggaccagccc
      961 gggcggcggc ggctcagggt aaaggcaacg gtagcagggc ccgcccgcct tcgggtagaa
     1021 gaccgacgcc tgcccgtcga agcggaagat cgacccgtag acattcggct tgcccaggag
     1081 cacgctcgcg tcgttggtca gatagcgcgt cgggaagttg tcggtcccgt cgatgatcac
     1141 gtcatagccc ttgaggatgg cgaaggcgtt ctcagccttg agcgccgtct cgtgggcgtc
     1201 gatctggacg tgcgggttga ggtcggcgat ccggtcgcgc gccgagtcga tcttcggccg
     1261 gccgacgtcc cgcgtcccgt ggagcacctg gcgctggagg ttgctgtggt cgacgacgtc
     1321 gaagtctacc agcccgagcc gcccgacgcc cgcagccgcg aggtagagcc cgagcggcga
     1381 acccagcccg ccggtgccga tgaggagcac gctcgccgcc ttgagcttgc gctgcccctc
     1441 catgccgacc tcgggcatga tcaggtgacg gctgtagcgc gcgacctcct ccttggtcag
     1501 ctcgggcagg gcagccggtt tgatctcatc ctcggtgacc gcgcccccgg caattgacgg
     1561 gatgatgctg atctcgtcgg cctccttgat cgccgtctcc tcgtgctgct ggtagcggat
     1621 gtcctcgtcg ttgacgtaga cgttgacgaa gttgcgcagc ttgccctgcg cgtcgaagag
     1681 gtgcttgcgc aggtcggggt gctgcgcggt caggctggcg agcgcctcgc cgattttcgc
     1741 cccgtgcact tccaccgacg cctgcccgcc ggcgtactgg cggagcgctg tcgggatcga
     1801 tatcctgggc atggcccttc ctccttatgg ttattaagcc gtctcttcaa tttgaatcag
     1861 ctcttcctcg tcgaactgcg agcggtcatg acgcatcgac caggcgcgga cctcggcgga
     1921 gagaccgccg cgcaccgaga cgatgaggta agcccactcg gtgaaggggg cgtgcgccag
     1981 gtcgaagccc gaaggctccg ccgggtggtc cgggtgcgag tggtagtagc cccagacgcc
     2041 aagaccccgc cgccccgcct cgcgctccgc cttgaggtag tcgcgcgggt cgagctcgac
     2101 gcggttgtgc cgcgagtcgg gccgcacgtt ggcgagcggc aggacctcgc ggatgaccct
     2161 cacgccatcc tccagagtgc cgagcagcag cccaccgcac tcctccgggt aggtcgcctc
     2221 gccgtgcctc ttgatggcct ccaggtgctc gcctgtcaag ttgatgccca tagctttcag
     2281 ctctcagcca gaccaggtcc cccttctggc tgaccgctga ctgctgacgg ctgatcgccc
     2341 tcctcccaga agttttcgct caaatacttg ctcccgtcgt cgcagaggat cgtcacgatg
     2401 acaccctcct ccagccggga ggcgagccgg agcgcggcgg cgacgttggc cgcggctgag
     2461 atgccga
//
LOCUS       DASRIG010000058         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3422039, whole genome shotgun sequence.
ACCESSION   DASRIG010000058 DASRIG010000000
VERSION     DASRIG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3422039"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1334
                     /locus_tag="VFB52_01605"
     CDS             165..1334
                     /locus_tag="VFB52_01605"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HZO05057.1"
                     /translation="MGIADPSKRLEAMPPYKFQELEQQIADKRASGIDVISLGIGDPD
                     EPTYDYIVKAMQAAVADPANHQYPSNRGREEFRQAFADFYDRRFGVEIDPATEVIPAI
                     GAKECIYNLCFAFLDPGGVALASDPGYPVYTGGPILANATPHVMALEPDLGFAPDLAA
                     IPGDVAEKARLMFINYPNNPTGAVVPEGFFERVVSFAKEYEILVVHDNAYSETTYDGY
                     VAPSFLATPGAKEVGVEVFSLSKGYNMTGWRCAAILGNAEAISTYWRLKTNVDSGLFE
                     AVQIAGAAALEGPTGPLERMNQTYTRRRDLVVSALREIGVEVNAPKGTIYVWAPVPEG
                     HTSTSFCETVLEEAAVVISPGSMYGPSGEGFFRISLTTPDERIEEAVERMREHLS"
     gene            1616..2173
                     /locus_tag="VFB52_01610"
     CDS             1616..2173
                     /locus_tag="VFB52_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05058.1"
                     /translation="MSPAEQGLRELIEERKDWYEPMPAKGWRRIAESETTAQFTRGRL
                     DGQMEWFGLELEEDGKWGSNGSSSDCEPTTVLDDVGNIVTWSLARKQKRLTAKTRTLW
                     IDLGPGGCASGAAQNPRAHFRFRTLGNRLLMIAWLGSTPGTHTCQGTIEPPRKVRLPA
                     PLGELRLYDGSTFPPVSAGKTRTRY"
     gene            2212..2994
                     /locus_tag="VFB52_01615"
     CDS             2212..2994
                     /locus_tag="VFB52_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05059.1"
                     /translation="MLVFALAILGIAAIALASDSDAETPVANPDPALVEWPEWPYRAT
                     CGFMGFDPVRVFSGPAAAEHGDGAPETALRDMLARDFPFPLPHHGWRMAARRNGKALF
                     LHGRLGAEFESPNQLENIELKHRGGRWQIETFGSFCNMFTVSQGRDAGGWRLAPKQPP
                     LAPSTERLQVITGARCSRAQKPPRVTPSFDEIAGKLVMTLWLRYPKTGGNGNEICSPG
                     LPPWPPVAIELPEPLGDRPLYDGAFFPPVPAERYERPTVIPL"
     gene            3035..3655
                     /locus_tag="VFB52_01620"
     CDS             3035..3655
                     /locus_tag="VFB52_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05060.1"
                     /translation="MSWKLTFLAAVAIVAIAGVAVAATGGGSKDVTICAQKKGGGLSL
                     APKSGKCGKDAKKLVIAKEGPAGPAGAPGAAGTTASIQPEAVRFVTPPTANGCEANPG
                     SFCGVTSEGGAKWHDWEDWGSVGFQKDAAGYVHLQGGTAFGGSGQTDSRIFFLPPGYR
                     PSAKTQVAATECDGTPISVFIGTNGEVLPHPVNFLCVILDGISFRP"
     gene            complement(3657..4514)
                     /locus_tag="VFB52_01625"
     CDS             complement(3657..4514)
                     /locus_tag="VFB52_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO05061.1"
                     /translation="MRVIDVMHLGRPHVIGCWEVDGMLVDPGPESSLPTVLEAIGGEQ
                     PIAVLLTHIHLDHAAATGAMVERWPDLEVYVHERGAPHLIDPSKLLASAERLYGDKLE
                     YLWGKILPVPEANVHVLSGGETALGMRVAYTPGHASHHVCYLHEETGTAFVGDTAACR
                     IPPSNFIMPPTPPPDIDIELWEESLDTIADWQPQQLALTHFGAIEDDVPGYIEAVRGT
                     LRKEAEFARDLPQYAYEISLGERIRGQLAIEGIGGETLEEILQAVPTAYQWPGLDRYW
                     RKKAEREAA"
     gene            complement(4605..5600)
                     /locus_tag="VFB52_01630"
     CDS             complement(4605..5600)
                     /locus_tag="VFB52_01630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO05062.1"
                     /translation="MKVFVTGGTGFIGGAVVRQLRARGDEVVCLVRNPEKGREAAELG
                     CEMVAGDLGDERAIRDGMAGCDAVIHAAAIYEVGIPAREREPMREANVGGTERVLGAA
                     LEAKIPKVVYVSTVGAFGNTDGKIVDETYEHPAESFTSCYEQTKWEAHQVAKKLIGEG
                     LPCTIVQPGGVYGPGDTSSIAVMLNEFLKGKMIAIPFPDLGICLTHVEDIAAGILLAL
                     DKGRAGESYVLSGPATTVREAIGVVADVTGKKAPKRALPTPLMKALTPVGPLVGKMMG
                     QPPNLRELISSADGVTFWATYDKAKNELGYEPRGLEDGFRAMLEAEGRLSASGQR"
     gene            5647..>6464
                     /gene="hflX"
                     /locus_tag="VFB52_01635"
     CDS             5647..>6464
                     /gene="hflX"
                     /locus_tag="VFB52_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZO05063.1"
                     /translation="MQRSRNGRKAETRYSAEHGNGGGVTNPRARQRALAIGIVDGSER
                     DNPLAELKELLRTAGVATAGEATQQREKPDPDRYFGRGRLEELKEEIKACDANLVACD
                     DELAPRQERNLESALGVPVIDRTAVILDIFADHAHSAEGKLQVELAQLEYNMARMRGL
                     WTHLERLGAGRMDGGIGTRGPGESQIETDRRLARNRIAALRRRLVRLEQNRGVMRAQR
                     DRSSLPRVALAGYTNAGKSTLLNAHTGAEVEVRDRLFHTLDPTTRSFEAGGRRYL"
BASE COUNT         1019 a         2247 c         2205 g          993 t
ORIGIN      
        1 cccgatcctg gtcgagctgg aaggtggcga gctggaggtc gagatcggcg aggacctggc
       61 ggtgcggctg accggctggg ccgagccggt ctacgcgggt gagctctcgc cggagttcat
      121 gcgggcgctc tccgagctcg actgaatcaa tagattccgc actcatggga atcgcagatc
      181 catccaagcg cctggaggcg atgcctccgt acaagttcca ggaactggag cagcagatcg
      241 ccgacaagcg ggcgtcgggc atcgacgtga tcagcctcgg catcggcgac ccggacgagc
      301 cgacctacga ctacatcgtc aaggcgatgc aggcggcggt cgccgacccg gccaaccacc
      361 agtacccgtc gaaccgcggg cgcgaggagt tccgccaggc cttcgccgac ttctacgacc
      421 gccgcttcgg cgttgagatc gacccggcga ccgaggtgat cccggcgatc ggcgccaagg
      481 agtgcatcta caacctctgc ttcgcgttcc tcgacccggg cggcgtcgcg ctcgcctccg
      541 atcccggcta cccggtctac accggcggcc cgatcctcgc caacgccacc ccgcacgtta
      601 tggcgctgga gcccgacctc ggcttcgccc cggacctcgc cgcgattccg ggggacgttg
      661 ccgagaaggc gcgcctgatg ttcatcaact acccgaacaa ccccaccggc gccgtcgtcc
      721 cggagggctt cttcgagcgc gtcgtctcct tcgccaagga gtacgagatc ctcgtcgtcc
      781 acgacaacgc ctactcggag acgacctacg acggctacgt cgcgcccagc ttcctcgcca
      841 cgcccggcgc caaggaggtc ggcgtcgagg tcttctcgct ctccaagggc tacaacatga
      901 ccggctggcg ctgcgccgcg atcctcggca acgccgaggc gatctcgacc tactggcggc
      961 tgaagaccaa cgtcgactcc ggcctcttcg aggccgtgca gatcgccggc gccgccgcgc
     1021 tcgagggccc gaccggaccg ctcgaacgga tgaaccagac ctacactcgc cgccgcgacc
     1081 tggtcgtctc cgccctgcgc gagatcggcg tcgaggtgaa cgccccgaag ggcacgatct
     1141 acgtctgggc cccggtcccc gagggccaca cctcgacctc cttctgcgag accgtgctcg
     1201 aggaggcggc ggtggtgatc tcgccgggct cgatgtacgg gccgagcggc gagggcttct
     1261 tccgcatctc cctcacgacc cccgacgagc ggatcgaaga ggcggtcgag cggatgcgcg
     1321 agcacctctc ctagcttcgc gggtccgtga cgacttggca gttcgctcgt agttgagttg
     1381 aatgacgccg atgctcgctc gctcgcatct cctcgctgcc ttcgccgccg cgacgattct
     1441 ctcgtttgcg gtcctcgtcg tcaccgcgcg gggctacggc gaacccacgc ctccggggcc
     1501 cgacccggct ctcgcgagct ggcccgattg gcccttccag gtgacctgca ggtcaggtga
     1561 agaatccttc gatccactgg tggcgtttgc cagcccgagc ggcgctgagc ggggaatgag
     1621 cccggccgag cagggcctgc gcgagctgat cgaggaacgg aaggactggt acgaaccgat
     1681 gccggcgaaa ggctggcgcc ggatcgccga atccgagacg accgcgcagt tcacgcgcgg
     1741 gcgcctcgac ggccagatgg aatggttcgg gctcgagctg gaagaggacg gcaagtgggg
     1801 ctccaacggg tccagcagcg actgcgagcc gacgacggtg ctcgacgacg tcgggaacat
     1861 cgtcacttgg agcttggcgc ggaagcagaa gcggctgacc gcgaaaaccc ggacgctctg
     1921 gatcgacctc gggccgggtg gatgcgccag cggcgccgcc caaaatcccc gcgcacattt
     1981 ccgcttccgg acgctgggta accggctgct tatgatcgcc tggttgggat cgacccctgg
     2041 aacccacacc tgccaaggga cgatcgagcc accccgcaag gtcagactgc cggcgccgct
     2101 gggagaactg cgcctctacg acgggtccac cttcccgccg gtctcggcgg ggaagacccg
     2161 cacccgctac tgaggatgtc gtccaggtcc gtccgctggc aggtcctgcc tgtgctcgtc
     2221 ttcgcgctgg cgatcctcgg gatagcggcg atcgcgctgg cctcggacag cgatgccgag
     2281 accccggtcg caaatccgga tccggccctg gtggaatggc ccgagtggcc ctaccgggcc
     2341 acctgcggtt tcatgggctt cgaccccgtg cgcgtctttt cgggtcccgc ggcggccgag
     2401 cacggcgatg gcgccccgga aacggcgctg cgcgacatgc tcgcgcgcga tttcccattt
     2461 ccgctcccgc accacggctg gaggatggcg gcgaggcgca acggcaaggc gctcttcctg
     2521 cacggcaggc tcggcgcgga attcgaatcg cccaaccagc tcgaaaacat cgaactgaag
     2581 caccgcggtg gccgctggca gatcgagact ttcgggagct tctgcaacat gttcacggtc
     2641 tcgcaggggc gtgatgcggg cggctggaga ctcgcgccga agcagcctcc gctcgccccc
     2701 tccacggagc gtctgcaggt gatcacgggt gcacgctgca gtcgcgctca gaagccaccc
     2761 cgggtcacgc catcgtttga cgagatagcc ggcaagctcg tgatgaccct atggctgaga
     2821 tacccgaaga ccggaggaaa tggcaacgaa atctgctcgc cggggctgcc accttggccc
     2881 cctgtcgcta tcgagctccc ggagccgctc ggcgaccgcc cgctctacga cggtgccttc
     2941 ttcccgccgg tcccggcgga acgctacgaa cgcccgacgg taattccgct ctaggggcct
     3001 tgccccattc ggtacccacc agtagggtcg cgcgatgagc tggaagctga cctttctcgc
     3061 cgcggtcgcg atcgtcgcga tcgccggagt cgccgttgcg gccacgggag gcggctcgaa
     3121 ggacgtcacg atctgcgccc agaagaaagg cggcgggctc agcctcgccc cgaagagcgg
     3181 caaatgtggc aaagacgcga agaagctcgt gatcgccaag gaaggccccg ctgggccggc
     3241 cggagcgccg ggtgccgccg ggaccaccgc ttcgatccag ccggaggcgg tccgtttcgt
     3301 caccccaccc acggccaacg gctgcgaagc gaatcctgga tccttctgcg gcgtcacctc
     3361 cgaaggtggt gccaaatggc acgactggga agactggggg tcggtgggct tccagaagga
     3421 cgcggccggt tacgtgcacc tgcagggcgg gacggcgttc ggtggctccg gccaaaccga
     3481 cagccgaatc ttcttcttgc cgccgggcta ccgcccgagt gcgaaaacgc aagtcgcagc
     3541 gacggagtgc gatggcaccc cgatctcggt ctttatcggc accaacgggg aagtgctgcc
     3601 gcaccccgtc aacttcctct gcgtgatcct cgacgggatc agcttccggc cctgagctag
     3661 gccgcttcgc gctcggcctt cttgcgccaa tagcggtcga ggccgggcca ctggtaggcg
     3721 gtcgggaccg cctgcaggat ctcctcgagc gtctcgccgc cgatgccttc gatcgccagc
     3781 tggccgcgga tccgctcgcc gaggctgatc tcgtaggcgt actggggcag gtcgcgggcg
     3841 aactcggcct ccttgcgcag ggtcccgcgt accgcctcga tgtagccggg gacgtcgtcc
     3901 tcgatcgcgc cgaagtgggt tagggcgagc tgctggggct gccagtcggc gatcgtgtcg
     3961 agcgattcct cccacagctc gatatcgatg tcgggcgggg gagtgggggg catgatgaag
     4021 ttcgagggcg ggatccggca ggcggcggtg tcgccgacga aggcggtgcc ggtctcctcg
     4081 tggaggtagc agacgtggtg ggaggcgtgg ccgggggtgt aggcgacgcg catccccagc
     4141 gcggtctcgc cgccgctcag cacgtgcacg ttggcctcgg ggaccggcag gatcttgccc
     4201 cagaggtact cgagtttgtc gccgtagagg cgctcggcgc tggccagcag cttcgagggg
     4261 tcgatcaggt ggggggcgcc gcgctcgtgc acgtagacct cgaggtcggg ccagcgctcg
     4321 accattgccc cggtcgcggc tgcgtggtcg aggtggatgt gggtgagcag aaccgcgatc
     4381 ggctgctcgc cgccgatcgc ctccagcacg gtcggaagcg aggactcggg gccggggtcg
     4441 acgagcatcc cgtcgacctc ccaacagccg atcacgtgcg gccggcccag gtgcatcacg
     4501 tcgatcacgc gcatcggagc caccctactg agcagcgtcg gggcggtgct agcaaggacg
     4561 cagggagcgt ggcgtgctct gcacgcgagc gaccgaggac gccgctagcg ctgtcccgac
     4621 gcgctcaggc ggccttcggc ttcgagcatg gcccgaaagc cgtcttcgag cccgcgcggt
     4681 tcgtagccga gctcgttttt cgccttgtcg taggtcgccc agaaggtgac gccgtcggcc
     4741 gaggagatca gctcgcgcag gttcggcggc tggcccatca tcttgccgac cagcggcccg
     4801 accggggtca gcgccttcat cagcggcgtc ggcagagcac gcttcggcgc ctttttgccg
     4861 gtcacgtcgg cgaccacgcc gatcgcctcg cgcaccgtcg tcgcggggcc gctgagcacg
     4921 taggactcgc cggcgcggcc cttgtcgagc gccagcagga taccggcggc gatgtcctcg
     4981 acgtgggtca ggcagatgcc gaggtcggga aacgggatcg cgatcatttt gcccttgagg
     5041 aattcgttca gcatcaccgc gatcgaagag gtgtcgccgg ggccgtagac gccgccgggc
     5101 tggacgatcg tgcagggcag gccctcgccg atcagtttct tggcgacctg gtgggcttcc
     5161 cacttggtct gctcgtagca ggaggtgaag ctctcggccg ggtgctcgta ggtctcgtcg
     5221 acgatcttcc cgtcggtgtt gccgaaggcg ccgacggtgg atacgtagac gaccttgggg
     5281 attttcgcct cgagcgccgc accgagcacc cgttcggtgc cgccgacgtt ggcctcgcgc
     5341 atcggctcgc gctcgcgggc cgggatcccg acctcgtaga tcgcggcagc gtggatgacc
     5401 gcgtcgcagc cggccatgcc gtcgcggatc gcgcgttcgt cgccgaggtc gccggcgacc
     5461 atctcgcagc ccagctcggc ggcctctcgg cccttctcgg ggttgcggac caggcagacg
     5521 acctcgtcgc cgcgcgcccg cagctggcgc acgaccgccc ccccgatgaa ccccgttcct
     5581 cccgtaacga aaaccttcaa ggttccccaa gcctagcccc ccgggtgggc gcctactatt
     5641 aagtcaatgc agagatcgcg gaacggtcgc aaggcggaga cgcgctacag cgccgagcac
     5701 ggcaacggcg gcggggtgac caacccgcgc gcccgccagc gcgcgctcgc gatcgggatc
     5761 gtcgacggct ccgagcgcga caacccgctg gccgagctga aggagctgct gcgcacggcc
     5821 ggggttgcga ccgccggtga ggcgacccag cagcgggaga aacccgatcc cgaccgctac
     5881 ttcgggcgcg ggcgcctgga ggagctgaaa gaggagatca aggcctgcga cgccaacctg
     5941 gtcgcctgcg acgacgaact cgccccgcgg caggagcgca acctcgagag cgccctgggc
     6001 gtccccgtga tcgaccgcac cgcggtgatc ctcgacatct tcgccgacca cgcccactcg
     6061 gccgagggca agctgcaggt cgagctggcc cagctcgaat acaacatggc ccgcatgcgg
     6121 ggtctctgga cccacctcga gcggctcggc gccggtcgta tggacggcgg catcggcacc
     6181 aggggcccgg gcgagagcca gatcgagacc gaccgccggc tcgcccgcaa ccggatcgcg
     6241 gcgttgcggc gccggctcgt gcggctcgaa cagaaccgcg gagtgatgcg ggcccagcgc
     6301 gaccgctcct cgctgccgcg cgtcgccctc gccggctaca ccaacgccgg caagtcgacg
     6361 ctgctgaacg cccacaccgg cgccgaggtc gaggtccgcg accgcctctt ccacaccctc
     6421 gacccgacga cgaggtcgtt cgaggcgggc ggccgccgct acct
//
LOCUS       DASRIG010000059         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1848033, whole genome shotgun sequence.
ACCESSION   DASRIG010000059 DASRIG010000000
VERSION     DASRIG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848033"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFB52_01640"
     CDS             complement(<1..718)
                     /locus_tag="VFB52_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05064.1"
                     /translation="MSSALAELIGEAHAFEDLEAFRQGIPGLLRHFVPADRASYNEMD
                     DDPRGSWWTADPAVTEAGPELEATFARLAGENPVLAYMRRTRDGRPYRISDFLSQPEY
                     HRLPLYREFYRHVGVEYQVAFSLPSRPPVVIGLALIRSSEDFTNPEVGLLGAARPHLI
                     QAYRNAELAAAREATIAALEARLEDVGTSVIVVDRYGRVDMATPSGRRLLAGRLGGTA
                     DRLAPDVIEALAARRAAGTPA"
     gene            787..1305
                     /locus_tag="VFB52_01645"
     CDS             787..1305
                     /locus_tag="VFB52_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZO05065.1"
                     /translation="MAIDTASIKDIAEKQCPQCFSGDLGWLLANANWALATEMTAALA
                     PLGLTARSYHVLRAASNGEYTQKALAEMVGIDKTTMVVTIDELEEAGFAERRPSPSDR
                     RAHVIAVTKSGERKLAKAEAVQAQVQGEALNALPTAQRKAFVEGLANMLEGLMADLPE
                     CTGAPRKREPRP"
     gene            1500..>2731
                     /locus_tag="VFB52_01650"
     CDS             1500..>2731
                     /locus_tag="VFB52_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO05066.1"
                     /translation="MPSLPSLASAPPAEGKDRRWTALLVLCAGMLMIILDQTIVNVAL
                     PSIQDDLGFSQSSLAWVINAYLIAFGGLLLLAGRLGDLLGPKRVFMTGLAVFVGASLL
                     CGLATSDAMLVGARFVQGVGGALTSAVILGMIVTMFPEPGERAKAIGVYSFVAAAGGS
                     LGLLLGGIITEAISWHWIFFVNLPIGIATAFFAARLVEGQQGIGLREGADALGAVLLV
                     SALMLGVYTIVEAANYGWGSLHTLGLGGVSIALLIGFVARQHTAADPLMPLRIFRSRN
                     VTGANAVQALMVAGIFGMFFLGAIYLQRVLGYGALEVGLAFLPVAGLIGLLSLGFSAK
                     LNMRFGPRATLLPGLTLILAGLLWFARSPVDASYLPDVLPAMVLLGLGAGLSFPSLMN
                     LAMSGAHQDDAGLASGLV"
BASE COUNT          371 a          978 c          929 g          453 t
ORIGIN      
        1 tcgccggcgt gcccgcagcg cggcgggcgg cgagcgcctc gatcacgtcg ggggcgagcc
       61 ggtcggcggt gccgccgagg cggccggcga gcaggcgccg cccggagggc gtcgccatgt
      121 cgacgcggcc gtagcggtcg accacgatca ccgaggtgcc gacgtcctcc aggcgcgctt
      181 ccagggccgc gatcgtcgcc tcgcgcgccg ccgccagctc ggcgttgcgg taggcctgga
      241 tcaggtgcgg gcgggcggcg ccgagcagcc ccacctcggg gttggtgaag tcctcggagg
      301 agcggatcag ggcgagcccg atcaccaccg gcggccgcga gggcaggctg aaggcgacct
      361 ggtactcgac cccgacgtgg cggtagaact cgcggtagag cggcagccgg tggtactcgg
      421 gctggctgag gaagtcggag atccggtagg gacggccgtc gcgggtgcgg cgcatgtagg
      481 cgagcaccgg gttttcgccg gccagccggg cgaaggtcgc ttccagctcc ggcccggcct
      541 cggtcaccgc cgggtcggcc gtccaccagg acccgcgcgg gtcgtcgtcc atctcgttgt
      601 aggaggcgcg gtcggcgggg acgaagtgcc gcagcaggcc ggggatgccc tggcggaacg
      661 cttccaggtc ttcgaaggcg tgggcttcgc cgatcagctc tgcaagcgcg gacgacacgg
      721 tttgcaaagg ctactagttg gagagcggac tatttggacg cggacctata tcgctaccat
      781 gtgcacatgg ccatcgacac cgcctcgatc aaggacatcg cggaaaagca gtgtccgcag
      841 tgcttctccg gcgacctcgg ctggctgctc gccaacgcca actgggcgct ggcgaccgag
      901 atgaccgcgg ccctggcgcc gctcggcctc accgcccgtt cctaccacgt gctgcgtgcc
      961 gcctcgaacg gcgagtacac ccagaaggcg ctggccgaga tggtcgggat cgacaagacc
     1021 acgatggtcg tcacgatcga cgagctcgag gaggccggct tcgccgagcg ccgtccctcc
     1081 cccagcgacc gccgcgccca cgtgatcgcg gtgaccaagt ccggcgagcg caagctcgcc
     1141 aaagccgagg cggtgcaggc gcaggtccag ggcgaagccc tcaacgcgct ccccaccgcc
     1201 cagcgcaagg cgttcgtcga aggcctcgcg aacatgctcg agggcctgat ggccgacctc
     1261 cccgagtgca ccggcgcccc gcgtaaacgc gagccgcgcc cgtagttcgc tgtcaattcg
     1321 ttcggaatag ccgaacaaat tgccatggcc tgggcctccg ggtcgccgcg cacacctagc
     1381 tgccctgggg gtgtgcgcgg tgacccggag ggcaatgctc cgtttgtaga tcgtctgtta
     1441 ccggacgatt tgctacggtt tgatcttcgt ccatacgaac tttgcaggag acccgctcga
     1501 tgccctcact tccctcactt gcttccgccc cgcccgcaga gggcaaagac cggcgctgga
     1561 ccgcgctgct cgtgctctgc gccgggatgc tgatgatcat cctcgaccag acgatcgtga
     1621 acgtcgctct gccgtccatc caggacgacc tcggcttctc gcagtcgagc ctcgcctggg
     1681 tgatcaacgc ctacctgatc gccttcggcg gtctgctctt actcgccggc cgactcggcg
     1741 acctgctcgg ccccaagcgc gtcttcatga ccgggctcgc cgtcttcgtc ggcgcctccc
     1801 tgttgtgcgg cctggcaacc agcgacgcga tgctggtcgg cgcccgcttc gtccaggggg
     1861 tcggcggtgc cctcacctcc gcggtgatcc tcgggatgat cgtgacgatg ttcccggagc
     1921 ccggtgagcg ggcgaaggcg atcggcgttt acagcttcgt cgccgccgcc ggtggctcgc
     1981 tcggcctgct gctcggcggg atcatcaccg aggcgatcag ctggcactgg atcttcttcg
     2041 tcaacctgcc gatcgggatc gcgaccgcgt tcttcgccgc ccgcctggtc gagggccaac
     2101 agggaatcgg cctccgcgag ggtgccgacg cgctcggcgc cgtgctcctg gtcagcgccc
     2161 tgatgctcgg cgtctacacg atcgtcgagg ccgccaacta cggctggggc tcgctgcaca
     2221 cgctcgggct cggcggcgtc tcgatcgccc tgctgatcgg cttcgtcgcc cgccagcaca
     2281 ccgccgcgga cccgctgatg ccgctgcgga tcttccgctc ccgcaacgtc accggcgcca
     2341 acgccgtgca ggcgctgatg gtcgccggga tcttcgggat gttcttcctc ggtgcgatct
     2401 acctgcagcg cgtgctcggc tacggcgcgc tcgaggtcgg cctcgccttc ctcccggtcg
     2461 ccggcctgat cgggctgctc tcgctcggct tctcggcaaa actgaacatg cgcttcggcc
     2521 cgcgcgccac cctgctgccc gggctgaccc tgatcctcgc cgggctgctc tggttcgccc
     2581 gctcgccggt cgacgccagc tacctgcccg acgtgctgcc ggcgatggtc ctgctcgggc
     2641 tcggcgccgg gctcagcttc ccctcgctga tgaacctggc gatgtcggga gcccaccagg
     2701 acgatgccgg gctcgcctcc ggtctggtca a
//
LOCUS       DASRIG010000060         7485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1848276, whole genome shotgun sequence.
ACCESSION   DASRIG010000060 DASRIG010000000
VERSION     DASRIG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848276"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFB52_01655"
     CDS             complement(<1..398)
                     /locus_tag="VFB52_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZO05067.1"
                     /translation="MIADLAKQQHGVVARRQLLDLGMSRHAIDGRLARGSLHQFHLGV
                     YVVGARRISRRGRWMAAVLACGPGAVLSHRAAGCLWGVLPPGFDRPEVTVPSGRRARR
                     LGIVCHEADIAADEVTEIDGIPVTSIFRTL"
     gene            563..717
                     /gene="ssrS"
                     /locus_tag="VFB52_01660"
     ncRNA           563..717
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFB52_01660"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(740..1036)
                     /locus_tag="VFB52_01665"
     CDS             complement(740..1036)
                     /locus_tag="VFB52_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05068.1"
                     /translation="MGLRDWLPRKWRPDPGPGKTPVHIDDPSFDSWEVVRDFGDLRTA
                     RAWHQSLIEAGFQAALTSDWPIDRFGHGDISLRVPPEQWSEAEWHLSNLDDELI"
     gene            complement(1051..2853)
                     /gene="aspS"
                     /locus_tag="VFB52_01670"
     CDS             complement(1051..2853)
                     /gene="aspS"
                     /locus_tag="VFB52_01670"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZO05069.1"
                     /translation="MSETTGAPPLRGNGYRDTWCAEVPGKVEGEARVAGWVNRRRDHG
                     GLIFIDLRDRTGIVQLVFHPDTSGAAFELAHKLRAEDVLSASGPVVKRDPETVNPELP
                     TGEVELSVSGAELLADAETPPFQIEGYSGEASEDVRMRYRYLDLRREQMRAALELRHR
                     VVSAMREFLDGEGFLDVETPMLTRSTPEGARDFLVPSSQQHGSFYALPQSPQLFKQLL
                     MMSGFERYYQVARCFRDEATRADRQAEFTQLDIEMSFVEGEDIIEVNERLLAHVFKSV
                     GGPEIELPMRRMCYDEAMGRFGTDRPDLRFGLELVDLGEALTGTEFKVFRSVLDGGGA
                     IRGLNAGKRELPRKDLDGLISRAQELGAKGLVWAFREGEGWRSPTAKFLSEAELADLN
                     QRLGAEEGDLLLLVADQRSVCDAVLGQMRIDLAEKFDLIDPKDNQLVWIVDWPLMEWN
                     EPEQRWDALHHPFTAPAGEFDPENPGAARAVAYDCVWNGQELGGGSIRINRADVQEQV
                     FAALGIDAETAKARFGFLLEALRYGAPPHGGIAYGVDRIVQRLYGAETIRDVIAFPKT
                     ASGFDLLTGAPAPVEEAQLKELGVAIHKRNLAGH"
     gene            complement(2850..4094)
                     /gene="hisS"
                     /locus_tag="VFB52_01675"
     CDS             complement(2850..4094)
                     /gene="hisS"
                     /locus_tag="VFB52_01675"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZO05070.1"
                     /translation="MASKFQAPRGTFDVLPAAARQRAAVEATARAIFARAGYESIQTP
                     SFEDTDLFERGVGRSTDIVRKEMFTFEDKGERSLTLRPEGTAPICRAYLEHGMHKLAQ
                     PVKLSYVGPFFRHERPQAGRYRQFHQLGLECIGTDSPLADAEAIMLLSDLLGELGVPG
                     VELRIGSLGSFAARQDYLEVLKAHLRKHEGDLSSDVRERIDVNPLRAFDADDPGTQGV
                     MASAPTIVDHLEGEDAEHFDEVKALLEAAGIDYVVDKTLVRGLDYYTRTIFSFVCDAL
                     GAQSEIGGGGRYDGLIEQLGGPATPAVGWAAGIERILLALDEEIEAEGRDAFVAIADP
                     DQRQRAMALTVEMRHAGLAAEVDLAGRGLKGQLKHADRIGARHVVILEADGTAQLRDM
                     QTGEQRPADTANLVDEMTGGEA"
     gene            complement(4133..4777)
                     /locus_tag="VFB52_01680"
     CDS             complement(4133..4777)
                     /locus_tag="VFB52_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO05071.1"
                     /translation="MSLDVQMLTVGPVAENCFLIRPEGSDQLLIVDPGEESDRILAAV
                     EESGADVAAILITHCHFDHIGAVAPVAAATGAPVYCPEIEAPVLADIMSYVPYPGFGP
                     FDSYDADETVAGGETLELAGLELDVVFTPGHSPGHVTYAVRGEDALFSGDVLFQGSVG
                     RVDLPGGDAPTLIASIGNLLDSFADEAVVYPGHMGTTTLGAERATNPFLTSLAR"
     gene            complement(4788..5156)
                     /locus_tag="VFB52_01685"
     CDS             complement(4788..5156)
                     /locus_tag="VFB52_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05072.1"
                     /translation="MAQTKKRQKGKKRGTQGGRVDSKPRGGRPRSREEAKQRASSKRS
                     VPTKGDQPPTWRNSVMRGLFAAVVFFVLLLLLFKKPIGSAATFSAFMLAFYIPAGYYI
                     DMFLWRKRERARIRARQQGK"
     gene            complement(5217..6113)
                     /locus_tag="VFB52_01690"
     CDS             complement(5217..6113)
                     /locus_tag="VFB52_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05073.1"
                     /translation="MAALLAVPNVSEGSDEGILQRLGEAFAAPAGLTLLDRHADADHG
                     RAVFTLAGEPGPLEEGLVRGAEEALETIDMNGYAGAHPAIGALDVCPLVWVAEDGREA
                     ARTAAAAVATQIGALGVPAFLYGELAQGPGRVERAYFRNGGLAELWLRMEAGELRPDF
                     GPPEPHRTGGAVLVTARPPLAAFNVELDSDDVEVARAVAAGLRESGGGLPGVRAMGLV
                     LGGGRAQISTNVHDPLEVPLGRVVEGVRELAAPLGARPLEAELVGLLPAAALAGYPED
                     VPIRGFDPARHVIERRLEAKAD"
     gene            complement(6124..7077)
                     /locus_tag="VFB52_01695"
     CDS             complement(6124..7077)
                     /locus_tag="VFB52_01695"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZO05074.1"
                     /translation="MSAELDRAIRTVVRQCMGVNPGEEVLVVCNPVTEEIGALMRIEA
                     QGDGADATLAVMSERDSAAAEPPRAVAAAMQAADVVLAPTIQSLSHTAARKEASEAGV
                     RVGSLPGVTEEMLVRLMNSDLEEIRRRGWAIVSAMNGGSEARITCRHGSDLRIGLEGR
                     LGIVDAGELSSRGAFGNLPCGEGFIAPLEGTAEGTLVVDGSIAEVGLLDTPTSLTIRE
                     GHLTDTTGSDGAALMELLTAHGPEATNVAELGIGTNEEAILTGNILEDEKILGTAHVA
                     FGASAAIGGTVQVPVHLDCVILEPTVEIDGETIVSGGDLLI"
     gene            complement(7193..7366)
                     /locus_tag="VFB52_01700"
     CDS             complement(7193..7366)
                     /locus_tag="VFB52_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05075.1"
                     /translation="MRPVLVAFLLVADAFVVGALVVAHPQGWVAPFIAFGAVLVGLVW
                     FQVWALRHRGDDN"
BASE COUNT         1066 a         2724 c         2571 g         1124 t
ORIGIN      
        1 aagagcgtgc ggaagattga ggtcaccgga atgccatcga tctccgtgac ttcgtcagcg
       61 gcgatgtccg cttcgtggca aacgatcccg agccgccgcg cgcgccggcc agacgggacg
      121 gtcacctcgg gacgatcgaa gccgggtggg agaacgcccc agagacagcc ggccgctctg
      181 tggctgagaa ccgcgccggg cccgcaagcg agcaccgctg ccatccaccg accgcgtcga
      241 ctgatccgac gcgcaccgac cacgtagacg cccaggtgga actgatgcag cgaaccgcga
      301 gcaaggcggc cgtcgatcgc gtggcggctc atgcccaggt cgagcagctg ccggcgtgcc
      361 acgacgccgt gctgctgctt cgcgaggtcg gcgatcaccc cgtcccagga tcgatccggc
      421 aatttgcccg gtataggcgg gtggattgcc gagtcgattt tcacccgcct gaggttggcg
      481 atccgcggcg cacctatggc gcgcagttcg tgccaattcc gtgccgagca agcacttcag
      541 gcgagggtgc taagttgggg ccaaggcccg agcccgtgcg cgagccgtct caccgtttga
      601 cccaacacaa acggcgcggg agccaccgta tccatggcct cttggggtcg ggatcaggca
      661 cccaccttca attgaaggga tcaaagccag aggcggcaac ggcgggctgg ggcctgattt
      721 atctcggccc cggcggggct cagatcagct cgtcgtcgag gttggagaga tgccactcgg
      781 cctcgctcca ctgttccggc gggacgcgca gggagatgtc gccgtggccg aagcggtcga
      841 tcggccagtc ggaggtgagt gcggcctgga agccggcctc gatcagcgac tggtgccagg
      901 cgcgggcggt gcgcaggtcg ccgaagtcgc gcacgacctc ccagctgtcg aagctcgggt
      961 cgtcgatgtg gaccggggtc tttcccggtc ccgggtccgg gcgccatttg cgcggcaacc
     1021 agtcccggag acccatcgct cagatgctcc tcagtggccg gcgaggttgc gtttgtggat
     1081 cgcgacgccg agttctttca gctgcgcctc ttcgaccggg gccggggcgc cggtgaggag
     1141 gtcgaacccg gaggcggtct tcgggaaggc gatcacgtcg cggatcgtct ccgccccgta
     1201 gagccgctgc acgattcggt cgacgccgta ggcaatgccg ccgtgcggag gtgccccgta
     1261 gcgcagcgcc tcgagcaaga agccgaagcg cgccttcgcg gtctcggcat cgatcccgag
     1321 cgcggcgaag acctgctcct ggacgtcggc gcggttgatc cggatcgagc cgccgccgag
     1381 ctcctgcccg ttccagacgc agtcgtaggc gaccgcgcgc gccgcccccg ggttctcggg
     1441 gtcgaactcg ccggccggcg ccgtgaacgg gtggtgcagg gcgtcccagc gctgctcggg
     1501 ctcgttccac tccatcagcg gccagtcgac gatccagacc agctggttgt ccttggggtc
     1561 gatcaggtcg aacttctcgg ccaggtcgat ccgcatctgc ccgagcaccg cgtcgcagac
     1621 ggagcgctgg tcggcgacca gcagcagcag atcgccctcc tcggcgccga ggcgctggtt
     1681 gaggtcggcg agctcggcct cgctgaggaa cttggcggtc ggcgagcgcc atccctcgcc
     1741 ctcgcggaag gcccagacca accctttcgc gcccagctcc tgggcgcgcg agatcagccc
     1801 gtcgaggtct ttgcgcggca gctcccgctt gccggcgttg aggccgcgga tcgcgccgcc
     1861 gccgtcgagc accgagcgga agaccttgaa ctcggtcccg gtcagcgcct cgccgaggtc
     1921 gaccagctcg agcccgaagc gcaggtcggg ccggtcggtc ccgaaccggc ccatcgcctc
     1981 gtcgtaacac atgcgccgca tcggcagctc gatctcgggt ccccccacgc tcttgaacac
     2041 gtgcgcgagc aggcgctcgt tgacctcgat gatgtcctcg ccctcgacga acgacatctc
     2101 gatgtcgagc tgcgtgaact cggcctggcg gtcggcccgg gtcgcctcgt cacggaagca
     2161 gcgcgccacc tggtaatagc gctcgaaccc gctcatcatc agcagctgct tgaacagctg
     2221 cggcgactgc ggaagcgcgt agaaggagcc gtgctgctgg ctcgacggga cgaggaagtc
     2281 gcgcgcaccc tccggggtcg agcgggtcag catcggcgtc tccacgtcga ggaagccctc
     2341 gccgtcgagg aactcgcgca tcgccgagac gacccggtgg cgcagttcca gtgccgcccg
     2401 catctgctcg cggcgcaggt cgaggtagcg gtagcgcatt cgcacgtcct cgctcgcctc
     2461 gcccgagtag ccctcgatct ggaacggcgg cgtctccgcg tcggcgagca gctcggcgcc
     2521 gctgacgctg agctcgacct cgccggtcgg cagctccggg ttgaccgtct ccgggtcgcg
     2581 cttgaccacg ggcccgctcg cgctcagcac atcctcggcg cgcagcttgt gggccagctc
     2641 gaacgccgcg ccggaggtgt cgggatggaa gacgagctgg acgatcccgg tgcggtcgcg
     2701 caggtcgatg aagatcagcc cgccgtggtc gcggcggcgg ttgacccagc cggcgacgcg
     2761 cgcctcgccc tcgaccttcc cgggcacctc cgcgcaccag gtgtcgcggt agccgttgcc
     2821 gcgcagcggc ggcgcgcccg tggtctcgct catgcttctc ctccggtcat ctcgtcgaca
     2881 aggttggcgg tatcggccgg gcgctgctct ccggtctgca tgtcgcgcag ctgcgccgtg
     2941 ccgtcggcct cgaggatcac cacgtggcgg gcaccgatcc ggtcggcgtg cttgagctgg
     3001 cccttcagcc cgcgcccggc gaggtcgacc tcggcggcga gcccggcgtg gcgcatctcg
     3061 accgtgagcg ccatcgcccg ctggcgctgg tccgggtcgg cgatcgcgac gaacgcgtcg
     3121 cgcccctccg cctcgatctc ttcgtcgagc gccagcagga tccgctcgat cccggccgcc
     3181 cagccgaccg ccggcgtcgc cgggccgccg agctgctcga tcaggccgtc gtagcggccg
     3241 ccgccgccga tctccgactg cgccccgagc gcatcgcaaa cgaaggagaa gatcgtgcgc
     3301 gtgtagtagt cgaggccgcg gaccagggtc ttgtcgacca cgtagtcgat cccggccgcc
     3361 tccagcagcg ccttcacctc gtcgaagtgc tcggcgtcct cgccctcgag gtggtcgacg
     3421 atcgtcggcg cgctcgccat cacgccctgg gtgccggggt cgtcggcgtc gaaggcgcgc
     3481 agcgggttga cgtcgatccg ctcgcgcacg tcgctcgaga ggtcgccctc gtgcttgcgc
     3541 aggtgtgctt tcaagacctc caggtagtcc tggcgcgccg cgaacgagcc aaggctgccg
     3601 atccgcagct cgaccccggg cacgcctagc tccccgagca ggtcggagag cagcatgatc
     3661 gcctcggcgt cggccagcgg cgagtcggtc ccgatgcact ccagcccgag ctggtggaac
     3721 tggcggtagc ggcccgcctg cgggcgctcg tggcggaaga acgggccgac gtaggagagc
     3781 ttcaccggct gcgccagttt gtgcattccg tgctcgaggt aggcgcggca gatcggcgcc
     3841 gttccctccg ggcgcagggt gaggctgcgc tcgcccttgt cctcgaaggt gaacatctcc
     3901 ttgcggacga tgtcggtcga gcggccgacc ccgcgttcga acaggtcggt gtcctcgaag
     3961 ctcggcgtct ggatcgactc gtagccggcg cgggcgaaga tcgcccgcgc tgtcgcttcg
     4021 accgcggcac gctggcgcgc cgccgccggc agcacgtcga aggtcccgcg cggtgcctgg
     4081 aacttgctgg ccatcgtgcg agcgagccta gagcagcggt cgagctggac gcctagcgcg
     4141 ccagcgaggt gaggaagggg ttggtcgcgc gctcggcgcc gagcgtcgtc gtgcccatgt
     4201 ggccgggata gacgacggcc tcgtcggcga agctgtcgag caggttgccg atcgaggcga
     4261 tcagggtggg cgcgtcgccg ccgggtaggt cgacccggcc gaccgagccc tggaacaaca
     4321 cgtcgccgga gaacagcgcg tcctcgccgc gcaccgcgta ggtgacgtgg ccggggctgt
     4381 gacccggggt gaagacgacg tctagctcca gcccggccag ctccagcgtc tcgccgccgg
     4441 cgacggtctc gtcggcgtcg tagctgtcga acggcccgaa gcccgggtag gggacgtagg
     4501 acatgatgtc ggcgagcacc ggcgcctcga tctcggggca gtagaccggc gccccggtcg
     4561 ccgccgccac cggcgccacc gcgccgatgt ggtcgaagtg gcagtgggtg atcaggatcg
     4621 cggcgacatc cgccccgctc tcctcaaccg cggcgaggat gcggtcggac tcctcgcccg
     4681 ggtcgacgat cagcagctgg tcggagccct cggggcggat caggaagcag ttctccgcca
     4741 ccggcccgac cgtcagcatc tggacgtcga ggctcaccgg cgtcgcctca tttgccctgc
     4801 tggcgtgccc ggatccgcgc ccgctcgcgt ttccgccaga ggaacatgtc gatgtagtac
     4861 ccggcgggaa tgtagaaggc gagcatgaag gcggagaagg tggcggcgct gccgatcggt
     4921 tttttgaaca gcaggagcag caggacgaag aagaccacgg cggcgaacag cccgcgcatg
     4981 accgagttgc gccaggtcgg cggttgatcg cccttggtcg ggacgctgcg cttcgagctg
     5041 gcccgttgct tggcctcctc gcggctgcgc gggcgcccgc cacgcggttt ggagtcgacg
     5101 cgaccaccct gggtgccgcg tttcttgccc ttctgcctct tcttcgtctg tgccatctct
     5161 gaattgaatc taaccggccg cgttcatggg gtgcgggtcg cggggtcgcc ggtcgctcaa
     5221 tcggccttcg cttcgagccg tcgctcgatc acgtgccggg ccggatcgaa gccgcggatc
     5281 ggtacgtcct cggggtagcc ggcgagcgcc gcggccggga gcaggccgac cagctccgcc
     5341 tcgagtgggc gggcgccgag cggcgcggcc agctcgcgca ccccctccac caccctgccc
     5401 agcgggacct ccagcgggtc gtggacgttg gtcgagatct gggcgcggcc gccgccgagc
     5461 accagcccca tcgcccgcac gcccggcagc ccaccaccgg actcgcgcag cccggcggcg
     5521 accgcgcgcg cgacctcgac gtcatcgctg tcgagctcga cgttgaacgc cgccagcggc
     5581 gggcgcgccg taaccagcac cgcgccgccg gtccggtgcg gctccggcgg cccgaagtcc
     5641 ggccgcagct cccccgcctc catccgcagc cagagctcgg ccagcccgcc gttgcggaag
     5701 taggcgcgct cgacccgccc cggcccctgc gccagctccc cgtagaggaa ggcgggcacc
     5761 ccgagcgcgc cgatctgggt cgcgaccgcg gctgcggcgg tgcgggcggc ctccctccca
     5821 tcctctgcga cccagaccag cgggcagacg tcgagcgccc cgatcgccgg atgtgcgccc
     5881 gcgtagccgt tcatgtcgat cgtctccagc gcctcctcgg ccccgcgcac cagcccttcc
     5941 tccagcggcc ccggctcgcc ggcgagcgtg aacacggcgc ggccgtggtc ggcgtcggcg
     6001 tggcggtcga gcagggtcag gccggcgggc gccgcgaacg cttcgccgag ccgctggagg
     6061 atcccctcgt cgctcccctc cgagacgttg ggaacggcga gtagtgccgc catcgccccg
     6121 gcctcagatc agcaggtcgc cgccgctgac gatcgtctcg ccgtcgattt cgacggtcgg
     6181 ttcgaggatc acgcagtcga ggtggaccgg cacctggacg gtgccgccga tcgccgcgga
     6241 ggcaccgaag gcgacgtgcg ccgtgccgag gatcttctcg tcctcgagga tgttgccggt
     6301 gaggatcgcc tcttcgttgg tgccgattcc gagctcggcg acgttggtcg cctccggtcc
     6361 gtgcgcggtc agcagctcca tcagcgcggc gccgtccgag ccggtggtgt cggtcaggtg
     6421 gccctcgcgg atcgtcagcg acgtcggcgt gtcgagcagg ccgacttcgg cgatcgagcc
     6481 gtcgacgacc agcgtgccct ccgcggtccc ctcgagcggg gcgatgaagc cctcgccgca
     6541 gggcaggttg ccgaaggcgc cgcggctgct cagttcgccc gcgtcgacga tcccgagccg
     6601 gccctccagc ccgatccgca ggtcgctgcc gtggcggcag gtgatccgcg cctccgagcc
     6661 gccgttcatc gccgagacga tcgcccagcc gcgccgccgg atctcctcca ggtcgctgtt
     6721 catcagccgc accagcatct cctcggtgac gccgggcagg ctgccgaccc tcaccccggc
     6781 ctcgctcgcc tccttgcgcg cagcggtgtg ggagagcgac tggatcgtcg gcgccaggac
     6841 cacgtcggcg gcctgcatcg cggcggccac ggcccgcggc ggctccgccg ccgccgagtc
     6901 gcgctccgac atcaccgcca gcgttgcgtc ggcgccgtcg ccctgcgcct cgatccgcat
     6961 cagcgccccg atctcttcgg tcaccgggtt gcagacaacc agtacctcct cacccggatt
     7021 gacgcccatg cactgccgga cgacggtgcg gatggcccta tccagctcag cgctcacgcg
     7081 cccagcctat ccccaggaac caaaccccgc tgccagtttc cccctgcacc ccccgcatgc
     7141 cgggacagat tgcaggaatt cagtccacca aaacttcggg aacccgaaat tctcagttgt
     7201 cgtcgcctct atgccgcagc gcccagacct ggaaccagac cagccccaca agcaccgccc
     7261 caaaggcgat gaacggcgcc acccacccct gcggatgcgc gaccaccagc gcccccacaa
     7321 caaacgcatc cgcgaccagc agaaaggcca ccagcaccgg ccgcaactgc gaccaacccg
     7381 tctcactgcc gcgcggacgc ctcgccaccc gccaaggatt accatgcccc tccccgccgg
     7441 agtagctcag ctggttagag cagcggaatc ataatccgcg tgtcg
//
LOCUS       DASRIG010000061         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_31128, whole genome shotgun sequence.
ACCESSION   DASRIG010000061 DASRIG010000000
VERSION     DASRIG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31128"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFB52_01705"
     CDS             complement(<1..299)
                     /locus_tag="VFB52_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZO05076.1"
                     /translation="MLLAIETSCDDTCAAVLDGARILSNVISSQAKAHEGFGGVVPEV
                     AARHHLELATPVVQAALGEAGVELDEIEAVAVTAGPGLIGALLVGVSTAKAIAAAR"
     gene            complement(346..813)
                     /gene="rimI"
                     /locus_tag="VFB52_01710"
     CDS             complement(346..813)
                     /gene="rimI"
                     /locus_tag="VFB52_01710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZO05077.1"
                     /translation="MSETLQKPSELTQEVEVRRLAYSDLPAVIAIERRSFPTPWSLAM
                     FVLELSKPSGICLAAHSGDELLGYVICSRYDQVWHLMNIAVSPDHRRAGVAGRLLRRL
                     FEEARGVLPFTLEVRVSNHGAIEMYERFGFRSAGIRPRYYHDNGEDALIMWLD"
     gene            complement(785..1486)
                     /gene="tsaB"
                     /locus_tag="VFB52_01715"
     CDS             complement(785..1486)
                     /gene="tsaB"
                     /locus_tag="VFB52_01715"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZO05078.1"
                     /translation="MVVVGFDTATADTAACAWRDGEVLHESLLGLNPEGRPWHTTGLL
                     AEVEASAAAAGGWDGVDLIAVGLGPGSFTGLRVGIATAQGLAAALGLPARGVCTLDAI
                     GRGIGEAGAEGERLVVQDGFRGEVFAALYGADGGRLWEPAVYRPEQLSERISSLRTAP
                     SVAGSGAVRFRSELSESAVRIPDDADPVHRVAARHVSALAAEAPEGGADQLAPIYLRP
                     PDAERWRERDSSKAE"
     gene            complement(1499..1870)
                     /locus_tag="VFB52_01720"
     CDS             complement(1499..1870)
                     /locus_tag="VFB52_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05079.1"
                     /translation="MKAVKALAAVCVVSGLLASAVPASADAAAYKHYVGCGISRNAEP
                     SHKCPAGSKKGAFFKSNNADVSYTVCVKFPSGKNLCAQAQEAKQGTLYVNKITSTIPG
                     VHTVSWFVKGKKVGSFRFRVI"
     gene            complement(1983..2399)
                     /gene="tsaE"
                     /locus_tag="VFB52_01725"
     CDS             complement(1983..2399)
                     /gene="tsaE"
                     /locus_tag="VFB52_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZO05080.1"
                     /translation="MERLESSSAAETEALGAKVAERLVPGDVVVVSGEVGAGKTTLIR
                     GACRALGVSEPVTSPTFTIGQRYAGGRLPISHLDLYRLESLEGEDPALLDDYLGADGV
                     AFVEWPAAGAGRLGRPALEISLAHEDGGRRRIEIDW"
     gene            complement(2401..>3084)
                     /locus_tag="VFB52_01730"
     CDS             complement(2401..>3084)
                     /locus_tag="VFB52_01730"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZO05081.1"
                     /translation="SPAAEERREQLVALYREVSKCTRCALHETRTKTVFGAGNADAEL
                     MFVGEAPGAEEDRQGLPFVGRAGQLLNQLLEEIGMQREDVFIANVLKSRPPGNRDPLP
                     EEIQACEPYLFEQVRLIEPKVVCTLGNFATKLLTGSPTGITRVRGTPQVHRLGGRTVF
                     LLPLLHPAAALRTPSMKETLRADFATIPALLAGPPAEPVPSAEEIAELEAEGGSTPPP
                     PADQLDMFG"
BASE COUNT          451 a         1089 c         1093 g          451 t
ORIGIN      
        1 cgcgccgcgg cgatcgcctt cgcggtgctg acgccgacca ggagggcgcc gatcaggccc
       61 ggcccggcgg tgaccgcgac cgcctcgatc tcgtccagct ccaccccggc ctcgccgagc
      121 gccgcctgga ccaccggcgt cgccagctcc aggtggtgac gggcggcgac ctcgggaacg
      181 acgccgccga agccctcgtg ggccttcgcc tgcgaggaga tcacgttgct gaggatgcgc
      241 gcgccgtcga gcacggccgc gcaggtgtcg tcgcaggaag tctctatcgc gagcagcatg
      301 ggaccgaagt tcagcaggtc gggagcgccg gactcagtcc agccctcagt ccagccacat
      361 gatcagcgcg tcctcgccgt tgtcgtggta gtagcgagga cggatgccgg cggagcggaa
      421 gccgaagcgt tcgtacatct cgatcgcgcc gtggttggag acgcgcacct cgagcgtgaa
      481 cggaagcacc ccgcgcgcct cctcgaacag ccggcgcagc agccggccgg cgacgccggc
      541 gcggcggtgg tccggggaga cggcgatgtt catcaggtgc cagacctggt cgtagcgcga
      601 gcagatcacg tagccgagca gctcgtcgcc ggagtgggcg gcgaggcaga tccccgaggg
      661 cttggaaagc tccagtacaa acatcgccag cgaccacggg gtcgggaagg agcgccgctc
      721 gatcgcgatc acggcgggca gatcgctgta ggcgagccgc cggacctcga cctcttgggt
      781 cagctcactc ggcttttgaa gagtctcgct cacgccaccg ctccgcgtcg gggggcctta
      841 ggtagatcgg tgcgagctgg tcggccccac cctccggcgc ttccgccgca agcgcgctga
      901 cgtgccgcgc ggcgacccgg tggacggggt cggcgtcgtc cggaatccgg acggcgctct
      961 cgctcaactc actccgaaat cgtaccgccc ccgacccagc cacagacgga gcggttcgca
     1021 atgacgagat tcgttcggaa agttgctccg gtcggtacac cgccggctcc cagagccgac
     1081 ccccgtcggc gccgtagagc gccgcgaaga cctcgccgcg gaagccgtcc tggacgacca
     1141 ggcgctcgcc ctccgccccg gcctcaccga tcccgcggcc gatcgcgtcg agcgtgcaga
     1201 cgccacgcgc cggcagcccg agcgccgcgg cgagcccctg ggcagtcgcg atcccgaccc
     1261 gcagcccggt gaacgagccg ggcccgagcc cgaccgcgat caagtccacc ccgtcccagc
     1321 cgccggcggc ggccgccgaa gcctcgacct cggcgagcag cccggtcgtg tgccacggcc
     1381 tcccttccgg gttgaggccg agcagcgact cgtgcagaac ctcgccgtcg cgccaggcgc
     1441 aagcggcggt gtcagccgtc gcggtgtcga agccgactac gaccagggcg ccgcgcccct
     1501 agatgacgcg gaacctgaag gagccgactt tcttgccctt gacgaaccag gagacggtgt
     1561 ggacgccggg gatcgtcgag gtgatcttgt tcacgtagag cgtgccctgt ttggcttcct
     1621 gcgcctgggc gcagaggttc ttgccgctcg ggaatttgac gcagacggtg taggagacgt
     1681 cggcgttgtt gcttttgaag aaggcgccct tcttgctgcc ggcggggcat ttgtgcgaag
     1741 gctcggcgtt gcgggagatg ccgcagccga cgtagtgctt gtaggcggcg gcatcggccg
     1801 acgcgggcac ggcgctcgcc aggagtccgg agacgacaca gaccgccgca agcgctttca
     1861 ctgccttcat tgggtccttc ccttcgtgga tcgcagttcg agaacgcgat gctacccgtt
     1921 gcgttcgtcg tcagaaacac gggctatcgt cgtttggagc ggcccgcggt gccgcatccg
     1981 ggctaccagt cgatctcgat acgccgccgg cccccgtcct cgtgagcgag gctgatctcg
     2041 agcgccggtc gcccgagccg cccggcgccg gccgccggcc actcgacgaa ggcgacgccg
     2101 tcggcgccga ggtaatcgtc gagcagggcc gggtcctctc cctccagcga ctcgagccgg
     2161 tagaggtcga ggtgggagat cggcaggcgg ccaccggcgt agcgctggcc gatcgtgaag
     2221 gtcggcgagg tgaccggctc gctcaccccc agcgcccggc aggcaccgcg aatcagggtc
     2281 gtcttgccgg cgccgacctc gccagagacg acgacgacat cgccgggaac cagccgctcg
     2341 gcgaccttgg cgccgagcgc ttcggtctcg gcggcggagc tgctctcgag ccgctccacg
     2401 tcagccgaac atgtccaact ggtcggcggg cggcggcggg gtcgagccgc cttcggcctc
     2461 gagctcggcg atttcctcgg cgctcgggac cggctccgcg ggcggcccgg cgagcagcgc
     2521 cgggatcgtc gcgaagtcag cgcgcagcgt ctccttcatc gagggcgtgc gcagcgccgc
     2581 cgccgggtgg agcagcggca gcaggaacac ggtgcggccg ccgagccggt gcacctgcgg
     2641 ggtgccgcgg actcgggtga tcccggtcgg gctgccggtc agcagcttgg tcgcgaagtt
     2701 gccgagcgtg cagacgacct tcggctcgat cagccgcacc tgctcgaata ggtagggctc
     2761 gcaggcctgg atctcctccg gcagcgggtc gcggttgccg ggcgggcgcg acttgagcac
     2821 gttggcgatg aagacgtcct cgcgctgcat cccgatctct tcgagcagct ggttcaacag
     2881 ctggccggcc cggccgacga acggcagtcc ttgacggtcc tcttcggccc ccggcgcctc
     2941 gccgacgaac atcagctcgg cgtcggcgtt gccggccccg aagacggtct tggtgcgggt
     3001 ctcgtgcagc gcgcatctgg tgcacttcga gacctcgcgg taaagcgcga ccaactgctc
     3061 gcggcgctcc tcggccgccg ggct
//
LOCUS       DASRIG010000062         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2902709, whole genome shotgun sequence.
ACCESSION   DASRIG010000062 DASRIG010000000
VERSION     DASRIG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902709"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..520
                     /locus_tag="VFB52_01735"
     CDS             185..520
                     /locus_tag="VFB52_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZO05082.1"
                     /translation="MQIQQRSSGNVTILDLNGNLTIGRSEESFRETVRELLAAERKLL
                     LVNLAEVPMVDSSGIGALIKAFTSVRDAEGKLKLLRPSRLARQLLTITGLLSILETYE
                     DESAAVASF"
     gene            612..>2091
                     /locus_tag="VFB52_01740"
     CDS             612..>2091
                     /locus_tag="VFB52_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZO05083.1"
                     /translation="MQKPAQLKVTNRFGVEKRLPLGKPVFTIGRKAENDLQLLSDSVS
                     RQHAEIAYQDETYYLIDRGSKRGSYVNDQRVERCQLQHLDQIRIGGDDHHQIQFLDET
                     VEQAAAIFRTNSSPRLSQSQWADRARAASANEELQKLARFVEVNQAFKFSLTPDDVLC
                     LIVDAAIEMTQAERGFLMLRNEHGDLEFKVARDRDRNWLVGNDFAMSHTVVEESFKQN
                     RSVIINDSREHALLAARQSVRSLDLRSIACIPLRRFQMSEHMDATTVLKRDVIGVLYV
                     DSHVISGTISQTSLRLLESLAFEATKSLESVRLMHEEQEKKQLEVELNMAREVQVALS
                     PVFRQPAHFEVAAQSIPSRYVGGDFYDLMPLAAGGTAFALGDVSGKGIAAALLAAMAQ
                     GALQAQFAGGLGLTEVINSLNRVLVQRSAADRFITLFCAVLDPDGRFAYINAGHNLPI
                     LVRTDGRTELLTTKSVLLGAFDFAKYQERETRLAPGDIVVIYT"
BASE COUNT          366 a          767 c          636 g          322 t
ORIGIN      
        1 ctcgggctgg cgatcgtcag gcacctcgtc gagctccacg gcggcgcggc cgtgctcgcg
       61 agcgaggtcg gccgcggcag ccgcttctcc ttcaccctgc cccgctaaag cggttataat
      121 cgctggcgcc ggcccctgcc cccagggccg gcgatctttg aaccgaggtg gacgcaagag
      181 attcatgcag attcaacagc gcagtagtgg gaacgtcacg atccttgatt tgaacggaaa
      241 tttaaccatc ggccggagcg aagagagctt tcgcgaaacg gtccgggagc tgctcgcggc
      301 cgagcgcaag ctcctcctcg tcaacctggc cgaagtgccc atggtcgaca gctccggcat
      361 cggcgccctg atcaaagcct tcaccagcgt gcgggacgcc gaggggaagc tcaagctgct
      421 ccgcccctcc cgcctcgcgc gccagttgct cacgatcacc ggcctgctct cgatcctcga
      481 gacctacgaa gacgagtccg ccgcggtcgc gtcattctga gagcggtcag cggtcagctt
      541 gcagctctca gccagagaag gggcgccggt ctggctgaga gctgaccgct gaccgctgac
      601 cgctgaccgc tatgcagaaa ccagcccagc tcaaagtcac gaaccgcttt ggggtcgaga
      661 agcggctgcc gctcggcaag cccgtcttca ccatcgggcg caaggcggag aacgacctgc
      721 agctgctctc cgactcggtc tcgcgccagc acgccgagat cgcctaccag gacgagacct
      781 actacctcat cgaccgcggg agcaagcgcg ggagctacgt caacgatcag cgcgtcgagc
      841 gctgccagct ccagcacctc gaccagatcc gcatcggcgg cgacgaccac caccagatcc
      901 agttcctcga cgagacggtc gagcaggctg cggcgatctt caggaccaac tcgagcccgc
      961 gcctctcgca gtcgcagtgg gccgaccgcg cccgcgccgc ctcggccaac gaggagctgc
     1021 agaagctcgc ccgcttcgtc gaggtcaacc aggctttcaa gttctcgctc accccggacg
     1081 acgtgctctg cctcatcgtc gacgcggcga tcgagatgac gcaggccgag cgcggcttcc
     1141 tcatgctgcg caacgagcac ggcgacctgg agttcaaggt ggcccgcgac cgggaccgca
     1201 actggctcgt cggcaacgac ttcgccatga gccacacggt cgtcgaggag tccttcaagc
     1261 agaatcggag cgtgatcatc aacgacagcc gcgaacacgc cctgctcgcc gcgcgccaga
     1321 gcgtgcgcag cctcgacctg cgctcgatcg cctgcatccc gctccggcgc ttccagatgt
     1381 ccgagcacat ggacgcgacc accgtgctca agcgcgacgt catcggcgtc ctctacgtcg
     1441 acagccacgt catcagcggg accatctccc aaacctcgct gcggctgctc gagtcgctcg
     1501 ccttcgaggc gactaagtcg ctcgagagcg tccgcctcat gcacgaggag caggagaaga
     1561 agcagcttga ggtcgagctc aacatggccc gcgaggtgca ggtcgcgctc tcgccagtct
     1621 tccgccagcc ggcgcacttc gaggtcgcgg cgcagagcat cccgtcgcgc tacgtcggcg
     1681 gcgacttcta cgacctcatg cccctcgccg ccgggggcac ggccttcgcc ctcggcgatg
     1741 tctcgggcaa ggggatcgcg gcggcgctcc tcgccgccat ggcgcaggga gcgctccagg
     1801 cgcagttcgc cggcggcctc gggctgaccg aggtcatcaa cagcctcaac cgcgtcctcg
     1861 tccagcgctc ggccgccgac cgcttcatca ccctcttctg cgccgtgctc gacccggacg
     1921 gccgcttcgc ctacatcaat gccggccaca atctgccgat cctcgtccgg accgatgggc
     1981 ggaccgagct gctgaccacc aagtcggtgc tcctcggggc cttcgacttc gccaagtacc
     2041 aggagcgcga gacccgcctc gccccgggcg acatcgtcgt catctacacc g
//
LOCUS       DASRIG010000063         5858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_33131, whole genome shotgun sequence.
ACCESSION   DASRIG010000063 DASRIG010000000
VERSION     DASRIG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33131"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VFB52_01745"
     CDS             <1..1485
                     /locus_tag="VFB52_01745"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZO05084.1"
                     /translation="SSKCSAPISYTHDPRTYTPGAQTVGDPLFPQIGNGGYDAKHYDL
                     RFNYDPAANVFLAGTQTTMRAVATQNLSRFALDFQPLAVSAVTIDGVPASFAFESTSP
                     PLGDPEAGVTQPTKLVITPSAGIKKGTTFKVVVAYSGEPVMFTDADESIEGWIPACYT
                     VAGVQTCDGAFTVNEPNGAQGYFPSNNIPSDKASIDTHTTVPNGKTALGSGELVSTVD
                     NGNGTTTWNWTEKYPMGIYLHTATVGDFNYSVTSMTETLTGRTLPLYRAFDSSATPAQ
                     ATALNKAFDEIPAQQNFVANLIGPYPFDSTGAVADRTTGVGYTLENQGKIHYSRLAIS
                     PGTQVHELTHMWMGDAVSPYRWNVIWFGEGWATFMEFFIEGAAASEEQFDAVYAAPAE
                     EWEVAPDDLAGDPANLFSGFPVYERPGATLEGFRQIVGDARFFKFAKAILANFKYGDI
                     DYARFVALAKQQSGLNKAGQAKLGEYFDQWLRQPTKPTLLPSDF"
     gene            1531..4389
                     /locus_tag="VFB52_01750"
     CDS             1531..4389
                     /locus_tag="VFB52_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO05085.1"
                     /translation="MASAGERAVEDRQGMAAVQASADLVERERELAEIARLVEATIAG
                     EGRMALIEGGAGVGKTRLLSEARRLAEGSGVAVLSARAGQLERDFPFGVVRQLFEPRL
                     HGEDWDARFVDAAAAAREVFEDLGEVEQSVGASYATLHGLFWLLLDLSEERPLVIAVD
                     DLQWCDPSSLRFLAFLVRRLEGMPILVAATMRSAEPGTDAALLSELAHDPFTESIEPG
                     PVSEDAARAMATLMLGAEADARFASACHEATGGNPLLLKELLTALRAERVEPVGQQAG
                     VVREVGRRSVSGSVQIRLARLSADAVAVARACAVFGEGATVAQVAGLTGLDERAAVVA
                     TEELTRAEILRPELPLGFVHPLVFDGVYGELSAGERELEHARAAELLRESGAVPDLVA
                     KQLLATPPRGNPDVARLLQEAGRAAQRKGAADSAVTYLSRALAEGPDPERRSQILFEL
                     GLAEALTNGPASVEHLRAAYEEVTDPVPKAVAAHVACRVLTFMESPREAADLARRTAE
                     LLPPELADLRQSFEVFEMVATILFGAGDVTSTNWRADRERLLAEGVGPGVGEKMLAAT
                     VAMFSANSDGDAETASKLALEALGDNLLIAADNGYMDILPMFVLAIADREEALEVADA
                     ILAEAQRRGSSFAVAALHGWRGFVLFYRGDLVGAEEAQRAGFEELQTWGISKTAGYYA
                     GGFLGITLIERGDLAGARALLEQTGTPEDSSDGARFWRNAMMWLLLAEGSNEAALVAA
                     EEFAELHSTVQNPATAWWRSVKAEALRRMDRGNEAIPLLEEQLELARRWGAPGTVGLA
                     LRELGAARQDEQGLAELEEAVAVLDGSPAKLELAEALCALGTALRHARRPADAREPLR
                     RALDLANACGAEPLAERSRAELNAAGGRARREALSGVESLTPSEQRVAGMAAEGMTNR
                     EIAQALFVTPKTVEVHLSNAYRKLGISSRRQLPAALPG"
     gene            complement(4392..5201)
                     /locus_tag="VFB52_01755"
     CDS             complement(4392..5201)
                     /locus_tag="VFB52_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05086.1"
                     /translation="MRSGLPTVLVVLAAFGLAACGGSGDSVGDEVREAAVLAGKSEDP
                     DEFCRQLVTERFVDEVFEGDLDACLESDVVGGDPGKPIVTAVVNPDGDDSQATVAMRF
                     EGGDADGVRGHLSFVDEEGEWRLDRFESDYLRSVFTVSLKAVDEGVLADPAVQDCMAK
                     RLERQDEQFIRTVMFLSLRDQKESETMTIGLVEECPGQVAGWVADETLAAYTQSGLDD
                     PAFLRCARRELEALLPLLDLGSDVIEADPEPASEAALEGVTLGVQENCEGG"
     gene            5256..>5858
                     /locus_tag="VFB52_01760"
     CDS             5256..>5858
                     /locus_tag="VFB52_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05087.1"
                     /translation="MRVADPGRFALKGAVRAAVVMPLAFALGDVVIGNPQTALFAAFG
                     SIALLVFVEFGGSMRARFSAYVGLALAGVATITLGTLCSHSTALGASAMFVVGFGILF
                     AGVLNGYVAAAQPAAILSFVLALMVPAASDQIPDRLAGWGIAALLATAATLLVWPQRP
                     RSVVRTGAARAARALADLLESDGEAERELRSTAARTAIRRA"
BASE COUNT          839 a         2190 c         2007 g          822 t
ORIGIN      
        1 agctccaaat gctcggcgcc gatctcctac acgcacgacc cgcgcacgta tacgccgggg
       61 gcgcagacgg ttggcgatcc gctcttcccg cagatcggca acgggggcta cgacgccaag
      121 cactacgacc tcaggttcaa ctacgacccg gccgccaacg tcttcctcgc aggcacgcag
      181 acgacgatgc gggccgtcgc gacgcagaac ctctcgcgct tcgcgctcga cttccagccg
      241 ctcgccgtct ccgcggtgac gatcgacggc gtcccggcga gcttcgcctt cgagtccacc
      301 tcgccgccgc tcggcgaccc cgaagccggc gtcacgcagc cgaccaagct cgtgatcacc
      361 ccgtcggcgg ggatcaaaaa aggcaccacc ttcaaagtgg tcgtcgccta ctcaggcgaa
      421 ccggtgatgt tcaccgacgc cgacgaatcg atcgagggct ggatccccgc ctgctacacg
      481 gtcgccgggg tgcagacctg cgatggggcc ttcacggtga acgagccgaa cggcgcccag
      541 ggctacttcc cctcgaacaa catcccgtcg gacaaggcca gcatcgacac ccacaccacg
      601 gtgccgaacg ggaagacggc gctcggctcc ggcgagctgg tctcgacggt cgacaacggc
      661 aacggcacga ccacctggaa ctggaccgag aaatacccga tgggtatcta cctgcacacg
      721 gccacggtcg gcgacttcaa ctacagcgtc acttcgatga ccgagacgct gaccgggcgc
      781 acgctgccgc tctaccgcgc cttcgactcc tcggcgacgc cggcccaggc gacggcgctg
      841 aacaaagcgt tcgacgaaat ccccgcccag cagaacttcg tcgccaacct gatcggcccc
      901 tacccgttcg actcgaccgg cgccgtcgcc gaccgcacca ccggggtcgg ttacacgctc
      961 gagaaccagg gcaagatcca ctactcgcgg ctcgcgatca gccccggcac ccaggtccac
     1021 gagctcaccc acatgtggat gggcgacgcg gtctcgccct accgctggaa cgtgatctgg
     1081 ttcggcgagg gctgggcgac cttcatggaa ttcttcatcg aaggcgccgc cgcctccgaa
     1141 gaacagttcg acgcggtcta cgcggcgccc gccgaagaat gggaagtcgc gcccgacgac
     1201 ctcgccggcg acccggccaa cctcttctcc ggcttcccgg tctacgaacg gcccggggcg
     1261 acgctggagg gcttccgcca gatcgtcggc gacgcccgct tctttaaatt cgcgaaagcg
     1321 atcctcgcca acttcaagta cggcgacatc gactacgcgc gcttcgtcgc cctcgccaag
     1381 cagcagagcg ggctgaacaa agccggtcag gccaaactcg gcgagtactt cgaccagtgg
     1441 ctgcgccagc caaccaaacc gacgctgctg cccagcgact tttagttcga agcacttcta
     1501 gctttgccgc gcctccccta ggatcgcggg atggcttcag ccggtgagag agcagtagag
     1561 gaccggcagg ggatggcggc ggtgcaggca tccgccgacc tggtcgagcg cgagcgcgag
     1621 ctggccgaga tcgcgcgcct ggtcgaggcg acgatcgccg gcgagggacg gatggcgctg
     1681 atcgagggcg gcgccggggt cggcaagacg cggctgctct cggaggcgcg gcggctggcc
     1741 gagggctccg gcgtggccgt gctgagcgcc cgcgccggcc agctcgagcg cgacttcccc
     1801 ttcggcgtcg tccgccagct gttcgagccg cgcctgcacg gggaagactg ggacgcgcgc
     1861 ttcgtcgacg ccgccgccgc ggcgcgcgag gtcttcgagg acctgggtga ggtcgagcag
     1921 agcgtcggcg cctcctacgc caccctgcac ggtctcttct ggctgctgct cgacctcagc
     1981 gaagaacggc cgctggtgat cgccgtcgac gacctgcagt ggtgcgaccc cagctcgctg
     2041 cgcttcctcg ccttcctcgt tcgccgcctc gaggggatgc cgatcctggt tgcggcgacg
     2101 atgcgctcgg ccgagccggg caccgacgcg gcgctgctct ccgagctcgc ccacgacccg
     2161 ttcacggagt cgatcgaacc ggggccggtc agcgaggacg cggcgcgggc gatggcgacg
     2221 ctgatgctgg gggccgaggc cgacgcccgc ttcgccagcg cctgccacga ggcgaccggc
     2281 ggcaacccgc tgctgctcaa agagctgcta acggcgctgc gcgccgagcg ggtcgagccg
     2341 gtcggccagc aggccggcgt ggtccgcgag gtcggccgcc gctcggtctc cggcagcgtc
     2401 cagatccggc tcgcgcggct ctcggccgac gcggtcgcgg tcgcccgcgc ctgcgcggtg
     2461 ttcggcgagg gcgcgaccgt cgcccaggtc gccggcctca ccgggctcga cgagcgcgcc
     2521 gccgtcgtcg ccaccgagga actgacgcgg gcggagatcc tgcgcccgga gctgccactc
     2581 ggcttcgtcc acccgctcgt ctttgacggc gtctacggcg agctctcggc cggcgagcgc
     2641 gagctcgagc acgcccgcgc cgccgagctg ctgcgcgagt ccggcgccgt cccggacctg
     2701 gtcgccaagc agctcctggc gacgccgccg cgcggcaatc ccgacgtcgc ccgcctgctg
     2761 caggaggccg gtcgcgccgc ccagcgcaag ggcgccgccg acagcgccgt cacctacctg
     2821 agccgggcgc tggccgaggg gcccgacccg gagcgccgct cccagatcct cttcgagctc
     2881 ggccttgccg aggcgctgac caacggcccg gcctcggtcg agcacctgcg cgccgcctac
     2941 gaggaggtga ccgacccggt gccgaaagcg gttgccgccc acgtcgcctg ccgcgtgctc
     3001 accttcatgg agtccccgcg cgaggccgcc gacctggccc gccgcaccgc ggagctgctg
     3061 ccgccggagc tggccgactt gcgccagtcc ttcgaggtct tcgagatggt cgcgacgatc
     3121 ctcttcggcg ccggcgacgt gaccagcacc aactggcgcg ccgaccgcga gcggctgctc
     3181 gccgagggcg tcggcccggg ggtgggggag aagatgctgg cggcgacggt ggcgatgttc
     3241 tccgccaact ccgacggtga cgccgagacc gcttcgaagc tggccctgga agcgctcggc
     3301 gacaacctgc tgatcgccgc cgacaacggc tacatggaca tcctgccgat gttcgtgctg
     3361 gcgatcgccg accgcgagga ggcgctggag gtggccgacg cgatcctcgc cgaagcccag
     3421 cgccgcggct ccagcttcgc ggtcgccgcc ctgcacggct ggcgcggctt cgtgctcttc
     3481 taccgcggcg acctggtcgg cgccgaggag gcgcagcggg ccggcttcga agagctgcag
     3541 acctggggga tctcgaagac cgccggctac tacgcgggcg gcttcctcgg gatcaccctg
     3601 atcgagcgcg gcgacctcgc cggcgcccgc gcgctgctcg agcagaccgg caccccggag
     3661 gactcctccg acggagcccg cttctggcgc aacgcgatga tgtggctgct gctcgccgag
     3721 ggcagcaacg aggcggcgct ggtcgccgcc gaggagttcg ccgagctgca cagcaccgtc
     3781 cagaacccgg cgaccgcctg gtggcgctcg gtcaaggcgg aggcgctgcg ccggatggac
     3841 cgcggcaacg aggcgatccc actgctcgag gagcagctgg agctggcccg ccgctggggc
     3901 gcgcccggca ccgtcggcct cgccctgcgt gagctcggcg ccgcccgcca ggacgagcag
     3961 ggactggccg agctggaaga ggcggtcgcg gtgctcgacg gcagcccggc gaagctggag
     4021 ctggccgagg cgctctgcgc gctcggcacc gccctgcgcc acgcccgccg ccccgccgac
     4081 gcccgcgagc ccctgcgccg cgccctcgat ctggccaacg cctgcggcgc cgagccgctc
     4141 gccgagcgct cccgggcgga gctcaacgcc gccggcggac gcgcccgccg cgaagcgctc
     4201 agcggggtcg agtcgctgac cccgagcgag cagcgcgtcg ccgggatggc cgccgagggg
     4261 atgaccaacc gcgaaatcgc gcaggccctg ttcgtcaccc cgaagacggt cgaggtccac
     4321 ctctccaacg cctaccgcaa gctcgggatc tcctcacgcc gccagctgcc ggcggcgctg
     4381 ccgggctgag ctcagccgcc ttcgcagttc tcctgcaccc ccagcgtcac gccttccagc
     4441 gccgcttcgc tcgccggctc ggggtcggct tcgatcacgt cggaaccgag atcgagcagc
     4501 ggcaggaggg cttcgagttc ccggcgtgcg cacctgagga aggccgggtc gtcgaggccg
     4561 gactgggtgt aggcggcgag cgtttcgtcg gcgacccagc cggccacctg gccggggcat
     4621 tcttcgacca gcccgatcgt catcgtttcc gactccttct ggtcgcggag cgaaaggaac
     4681 atgacggttc tgatgaactg ctcgtcctgg cgttcgagcc gcttcgccat gcagtcctgc
     4741 accgcggggt cggcgaggac gccctcgtcc accgccttca agctgacggt gaagaccgag
     4801 cgcaggtagt cgctttcgaa gcggtccagg cgccactcgc cttcttcgtc gacgaagctg
     4861 aggtggccgc ggacgccgtc ggcatcgccg ccctcgaagc gcatcgccac ggtcgcctgc
     4921 gagtcgtccc cgtccgggtt gacgacggcg gtgacgatcg gcttgcccgg atccccgccg
     4981 accacgtcgg actccaggca ggcgtcgagg tcgccctcga agacttcgtc gacgaaccgc
     5041 tcggtgacca gctggcgaca gaattcatcg gggtcttcgc tcttgccggc cagcaccgcc
     5101 gcctcgcgca cttcgtcccc caccgaatcg ccgctgccgc cgcaggctgc cagcccgaac
     5161 gcggcgagga cgacgagaac cgtggggagc cctgagcgca ccgagcttcg gctacccgcc
     5221 ggtggttcgg cgtaagcagg tgggaagatc ggcgcgtgag agtcgccgat ccaggccggt
     5281 tcgcgctcaa aggtgcggtg cgggccgcgg tcgtgatgcc gctcgccttc gcgctcggcg
     5341 acgtcgtgat cggcaatccg cagacggcgc tgttcgccgc cttcggctcg atcgcgctgc
     5401 tcgtcttcgt cgaattcggc ggctcgatgc gggcgcggtt ctcggcttac gtcgggctgg
     5461 cgctggccgg ggtggcgacg atcacgctgg gcacgctctg ctcgcactcg accgcgctcg
     5521 gcgcctcggc gatgttcgtc gtcggcttcg ggatcctctt cgccggcgtg ctcaacggct
     5581 acgtcgccgc cgcccagccg gcggcgatcc tctccttcgt cctcgccctg atggtgccgg
     5641 cggcgagcga ccagattccc gaccggctgg cgggatgggg gatcgccgcg ctgctcgcga
     5701 ccgccgcgac gctgctggtc tggccgcagc gcccgcgcag cgtcgtccgc accggcgccg
     5761 cccgcgccgc ccgcgccctc gccgacctgc tggaaagcga tggcgaggcc gagcgcgagc
     5821 tgcgctccac cgccgcccgc accgcgatcc gccgggcc
//
LOCUS       DASRIG010000064         8283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1591828, whole genome shotgun sequence.
ACCESSION   DASRIG010000064 DASRIG010000000
VERSION     DASRIG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591828"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFB52_01765"
     CDS             <1..458
                     /locus_tag="VFB52_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05088.1"
                     /translation="YREGLEQGGHDPATGAMGGLVSMIVSNDPERAWATIKPHLDYQW
                     RSYEEAAMDGVADADMPDLQAGTSIERSAGPIMLPPGWDAVTPDEAVRRLTAWLGDLP
                     VSDVFFFDNLSGMPDELVEEHLTLLATEVKPRLAAVGKVAPQAGSSQLP"
     gene            549..2147
                     /locus_tag="VFB52_01770"
     CDS             549..2147
                     /locus_tag="VFB52_01770"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZO05089.1"
                     /translation="MTLLVALAFVVAPAGASAKSYYATPSPEPGASCTAESPCALSTA
                     ISTAADGETVVIGAGTYSEPGAYPGADPVYADAAKDLTIEGAVIGVGRPVVNGVFLLT
                     GPGTRLSDVAIHSSWFEYSALDLGNGAHADRVVASSGAIDGCRIASSGGTVTNSLCSG
                     TSPSSGLSTQLRTGSGSINVRNATMIGKYAYYSGPVSGTTVSDSIAKRSTGLGADANM
                     NTGTTALVRTYALHQEGTALSEESPLTEEPTFREAGDFRVAAGSPSIDFGSTAAEPGE
                     LDLNGNLRKIGTKVDLGAYEFVPDPPSVSTPTVTGATTTSATVNATINPNSGRTYYYL
                     EYGSTTAYGTTTPTVAIPAATAPTAVQVDLSGLPADATLHYRLVATSDGGTTAATDGI
                     FETAPPPPPPPPAPPKKLAPILRLAQGKGGPPKGQPLLDRSAIKFSTGCGPIACDVRI
                     VGTVKIGAKTFGTLPGPKTPSHWQAGKQGAMKLRVSSKLQQSVRKHLEANAGARAKII
                     VTATYVTAAGATATRKLTIAVRPV"
     gene            complement(2187..2260)
                     /locus_tag="VFB52_01775"
     tRNA            complement(2187..2260)
                     /locus_tag="VFB52_01775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2224..2226),aa:Pro,seq:ggg)
     gene            2316..2386
                     /locus_tag="VFB52_01780"
     tRNA            2316..2386
                     /locus_tag="VFB52_01780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2348..2350,aa:Gly,seq:tcc)
     gene            2494..3690
                     /locus_tag="VFB52_01785"
     CDS             2494..3690
                     /locus_tag="VFB52_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZO05090.1"
                     /translation="MLVVLALVAVGCGGSDDGDGGAAAETAPAAEGGKLVDWPFFGRL
                     PERTHYLPTKEVNLDPPLRQAWSVNTHALIEFPPAIAGGVAYVVNKYGNGKAIKLSNR
                     EILRELNLRPSDKGHQLDVTAPVYHEGRVFGTYLDGYVAAGAAADGSKIWVRKLNTPI
                     ESSPLPIDELLYFGTNQEGVLALDTADGKTRWQFKTPGAVKSSPSYHDGRVFVADYES
                     TMFALDAKTGKEEWRSNTSQVAPGGRGGFFSSPAIGWGKVYAARDDGTVYAFDEKSGK
                     VAWSFPAGAAIYGSPALAEVPGTPATVYIGAENGRFYALDAKTGKPRWSYDVGGPIPG
                     TATVIGNTVYTSSFETDRTIGLDVHTHKKIFEIKQAGYTPVVSDGRRLFLIGYFAVVG
                     LESKRR"
     gene            complement(3726..4688)
                     /locus_tag="VFB52_01790"
     CDS             complement(3726..4688)
                     /locus_tag="VFB52_01790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZO05091.1"
                     /translation="MALFVVTTRLPSSALEMLRAAGELRAADVAGVLPRAELLELVAG
                     ADVVLTQLGDRVDDELLEAAGPGLRCVCNVAVGYDNVDLAAAARHGVAVTNTPGVLDD
                     ATADLAIALMLIATRRLGEGERLIRSGQPWHWSMEFMLGSGLRDKLLGVVGLGGIGSR
                     VAERARAFGMRIAYNNRGVAPAAVTEPLGAERLELDELLARADVLTLHCPLTDETRHL
                     IGAAQLAAMKPSATLVNTARGPVVDEAALVEALRSGEIAAAGLDVFEREPEVHPGLLE
                     LDNAVLVPHLGSATVETRTAMAELAARNAIAAANGEPLPTPVSS"
     gene            complement(4733..>8283)
                     /locus_tag="VFB52_01795"
     CDS             complement(4733..>8283)
                     /locus_tag="VFB52_01795"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HZO05092.1"
                     /translation="PAVEITEGPALNWGFKETWRNYAQKPQVSGGAAVVAAAGNAAYD
                     LEWEFESGSYDPATQTTRVSYSGSAHWQKYKASDLGRPKPPGYSGPETDPYLLDVTLA
                     DPQITIDRDSSVLTAVATSRNQDTWELISYGRVPIVNLDVLGVTPAVAGGETKWDGIT
                     ATVTDEGSAALANNYRVGQVVDAVGFSYTGPGGAPDFSEHWDQPGAAVLKLVENSLFI
                     DGEDVVDLQPLWVDRKNLIVHSTLSLPGSQVRIEAFDLKEMEPVGDPLVLPTSGAPAW
                     ATNVTAYDTSKDRLLFRANGESGMPRWFKFNLETEQYETGLLGDPQMSETAFIGANSQ
                     RLVWDPIRNRGYRVQRIVPAGVPANEYDNHEWTLFAYAEGENGVWTKKSFKLPSFPAG
                     QNQTGFPTTSLNPANYAVASDGSLIALASTRSGLAASEKIAGAYRIVFNQADDAVSVQ
                     PLPGVEVLNTILAGGTFSTVQTSANGHIVLVRMAAGGEVVHCQLDGQGGVGCDDPVSI
                     RDNVEAGPYDERRYALDPADGTIWFGGVTSQKLAAVRGNAFAGGQFFQERHPRGGPVL
                     VGDGHFVYAQTNDGSPGESGGSKTWGFGKFERLGFVPTVSGQPQPDAVALSSGEASEE
                     ATFSSTATGDPAPARQWQVKAPGSSKFVDVPGAVGETLSVAATRGDDGSEYRAVYSNA
                     AGKVVSDTAALSVDYAPLVLQSPPNAKVLEGADAEFAVLADGKPEPTVTWQRRVNGFW
                     ENVTAEDESLVVDGNSLTVLGANPDQSGAIFRARLANPLGTATSEAAKLTVTPKVTIP
                     DGGVDLSKVTLDWSGSAELQKAPPFGGSNYFSAGVSDGDEASFHGHEGNAFVFQVAAS
                     GAEAIATWATRQDHVGNGGSQLVRLYGGSGRVEEDGSAEVEWDGAFSVNFYGGLAPFT
                     VSDPVLTVDDEGEGVLSADLVGCASSMANPSECNPLPPVADVTVATFSGVEVDPAGTL
                     TVTPDYAGVEVTVPPGKTPQDRSGAGWGAWPQPFVDYHVETGLYSYWYSSGGAADPLK
                     APLPFAVDFAGADPPAVDDGPDDKGKGGGGGPEPKAPPVPVPPSIGAIKDVQEVGGKR
                     RAKVATLSCPGASPCVVKAPRNAPVRIGGRTYWAAVYAPRQIAAGASATVRARLAPAA
                     LRALGAGRATLRVQLQVRSDEGVARLLARVPLEAAG"
BASE COUNT         1234 a         2988 c         2818 g         1243 t
ORIGIN      
        1 cctaccgcga aggcctcgag cagggcggcc acgatccggc caccggcgcg atgggcgggc
       61 tcgtctcgat gatcgtctcc aatgacccgg agcgggcctg ggcaacgatc aagccccacc
      121 tcgactacca gtggcgctcc tacgaggagg ccgccatgga cggcgtggcg gacgccgaca
      181 tgccggacct gcaggcgggg acctcgatcg aacgctcggc ggggccgatc atgctgccgc
      241 ccggctggga cgcggtcacc cccgacgagg cggtgcgccg gctcacggcc tggctcggcg
      301 atctgccggt cagcgacgtc ttcttcttcg acaacctgtc cgggatgccc gacgagctgg
      361 tcgaggaaca cctgaccctg cttgccaccg aggtgaaacc gcgcctcgcg gcggttggca
      421 aggtcgctcc gcaggccggc tcttcgcagc tgccctagcc gcgtcgccac ggactggcct
      481 tcgcgatcct gttcgcgtgg ccgtcgccca cgaatggata ggatttccga atgggtcgaa
      541 gtctcggctt gactctcctc gtagcgctcg cctttgtcgt ggctcccgcc ggcgcgtcgg
      601 cgaaaagcta ctacgcgact ccgagcccgg aaccgggcgc gagctgcacc gccgaatcgc
      661 cgtgcgcgct ctcgaccgcg atcagcaccg cggccgacgg cgaaaccgtc gtgatcggcg
      721 ccgggaccta ctcggagccc ggagcctacc caggagcgga tccggtatac gccgacgcag
      781 ccaaggacct gacgatcgaa ggagcggtga tcggggttgg gcggccggtt gtgaacggcg
      841 tcttcctgct gaccgggcca ggaactcggc tcagcgacgt ggcgatccac tccagctggt
      901 tcgaatactc ggcgctggat ctcggcaacg gtgcccatgc cgatcgagtg gtggcgagca
      961 gcggggcgat cgacggctgc cggatcgcat ccagcggcgg aaccgtcacc aactctctct
     1021 gctcggggac ctcgccctcc agcggcctct cgacgcaact gcggacgggc tcgggctcga
     1081 tcaacgtacg aaacgccacg atgatcggaa aatacgcgta ctactcgggt cccgtgtcgg
     1141 ggacgaccgt gtcggactcg atcgcgaaga ggagcacggg cctcggcgcc gacgccaaca
     1201 tgaacacggg cacgacggcc ctggtgcgga cctacgcctt gcaccaagaa ggaacggctc
     1261 tgagcgaaga aagcccgctg accgaagaac cgaccttccg cgaagcgggc gatttccgcg
     1321 tggccgccgg ctcgccgtcg atcgacttcg gctcgaccgc cgccgaacca ggcgagctcg
     1381 acctcaacgg aaacctgcgc aaaatcggaa cgaaagtgga tctcggcgca tacgagttcg
     1441 tccccgatcc gccttcggtc agcaccccga cggtcaccgg ggccacgacc acgagcgcga
     1501 cggtcaacgc cacgatcaat ccaaactccg gccggaccta ctactacctc gaatacgggt
     1561 cgaccacggc gtatggcacc acgacgccga ccgtcgccat cccggcggct accgccccca
     1621 ccgccgttca ggtcgacctc agcgggttac ctgccgacgc caccctgcac taccggctcg
     1681 tcgcgacgag cgacggcggc accaccgcgg ccaccgacgg gatcttcgag accgcgcccc
     1741 caccgccgcc ccctccgccg gcgccgccca agaagctcgc cccgatcctg cgcttggccc
     1801 agggcaaggg cgggccgcca aagggccagc cgctgctcga ccgctcggcg atcaaattct
     1861 cgaccggctg cggcccgatc gcctgcgacg tcagaatcgt gggaacggtg aagatcggcg
     1921 ccaagacctt cggcaccctt ccggggccga agactccgag ccactggcag gcggggaagc
     1981 agggcgcgat gaagctcagg gtcagctcga agctgcagca gagcgtccgc aagcacctcg
     2041 aagccaatgc cggcgcccgg gccaagatca tcgtcaccgc cacctacgta accgcagccg
     2101 gcgcgacggc gacgcgtaag ctgacgatcg ccgttcggcc ggtctagccc gcggatcgcc
     2161 gccgcttcgc aggtggctct gaaatatcgg ggagacagga tttgaacctg cgaccgctcg
     2221 gcccccagcc gagtgcgcta ccagactgcg ccactccccg ttggggattg catcttacga
     2281 atctgcgagg ggccggctag gcttcccccc atcctgcggc ggtagctcaa cggtagagcc
     2341 tcagccttcc aagctgatga cgcgagttcg attctcgtcc gccgctctcc ttcaggcgtc
     2401 cagattgggc gggtccgctt cgggaacggg cgggtagtcc gatggacagg gatgcgcttt
     2461 ctttctcgcc ttcgccgttt ggtgctcggc ctggtgctgg tggtgttggc cctggtcgcg
     2521 gtcgggtgcg gcgggtccga cgatggggac gggggtgcgg cggcggaaac ggcgccggcg
     2581 gcggaaggcg ggaagctggt cgactggccg ttcttcgggc ggctgccgga gcgcacgcac
     2641 tacctgccga ccaaggaggt gaacctcgat ccgccgctgc ggcaggcgtg gtcggtcaac
     2701 acgcacgctc tgatcgagtt cccgccggcg atcgcgggtg gggtcgccta cgtggtcaac
     2761 aagtacggca acgggaaagc gatcaagctg agcaaccggg aaatcctcag ggaactgaac
     2821 ctgcggccga gcgacaaagg gcaccagctc gacgtgaccg cgccggtcta ccacgaaggc
     2881 cgggtcttcg ggacctatct cgacggctac gtggccgccg gggccgcggc cgacggcagc
     2941 aaaatctggg tgcgcaagct gaacacgccg atcgaatcct cgccgctgcc gatcgacgaa
     3001 ctgctctact tcggcaccaa ccaggagggc gtgctggcgc tcgacaccgc cgacggcaag
     3061 acgcgctggc agttcaagac gccgggcgcg gtgaaatcga gcccgagcta ccacgacggc
     3121 cgcgtcttcg tcgccgacta cgagagcacg atgttcgcgc tcgacgcgaa gaccggcaag
     3181 gaagagtggc gctcgaacac ctcgcaggtg gccccgggcg ggcgcggcgg cttcttctcc
     3241 tcccccgcga tcggctgggg caaggtctac gcggcccgcg acgacggcac cgtctacgcc
     3301 ttcgacgaaa agagcggcaa ggtcgcctgg tcgttcccgg ccggcgccgc gatctacggc
     3361 tcccccgcgc tcgccgaagt gcccggcacg ccggcgaccg tctacatcgg cgccgaaaac
     3421 ggccgcttct acgcgctcga cgcgaagacc ggcaagccgc gctggagcta cgacgtcggc
     3481 ggcccgatcc ccggcaccgc aaccgtgatc ggcaacaccg tctacacctc gagcttcgaa
     3541 accgaccgca cgatcgggct cgacgtccac acccacaaga agatcttcga gatcaagcag
     3601 gccggctaca ccccggtcgt ctccgacggc cgccgcctct tcctgatcgg ctacttcgcc
     3661 gtcgtcgggc tcgaatcgaa acggcgctag cgccggcgtg tcgactttgg cggccctatg
     3721 cgcgcctaac tcgacacggg ggtcggcagc ggctcgccgt tcgccgcggc gatcgcgttg
     3781 cgggccgcga gctcggccat cgccgtgcgc gtctccaccg tcgccgagcc gaggtgcggg
     3841 accagcaccg cgttgtccag ctccagcagc ccgggatgca cctccggctc gcgctcgaag
     3901 acgtcgagcc cggcggcggc gatctcgccg ctccgcagcg cctcgaccag cgccgcctcg
     3961 tcgacgaccg gcccgcgcgc ggtgttgacc agggtcgcgc tcggcttcat cgccgccagc
     4021 tgcgccgccc cgatcaggtg gcgcgtctcg tcggtcagcg ggcagtgcag ggtgagcacg
     4081 tcggcgcgtg ccagcagctc gtcgagctcc agccgctcgg cgccgagcgg ctcggtcacc
     4141 gcggccgggg cgacgccgcg gttgttgtag gcgatgcgca tgccgaaggc gcgggcgcgc
     4201 tcggcgaccc ggctgccgat cccgcccagc ccgaccaccc cgagcagctt gtcgcgcagg
     4261 ccgctgccga gcatgaactc catgctccag tgccacggct gcccggagcg gatcagccgc
     4321 tccccctccc ccagccgccg cgtcgcgatc agcatcaggg cgatcgcgag gtcggcggtg
     4381 gcatcgtcga ggactcccgg cgtgttggtc accgcgaccc cgtgccgcgc cgccgccgcg
     4441 aggtcgacgt tgtcgtagcc gacggcgacg ttgcagacgc agcgcagccc cggcccggcg
     4501 gcttccagca gctcgtcgtc gacccggtcg ccgagctggg tgaggacgac gtcggcgccc
     4561 gcgaccagct ccagcagctc cgctcgcggc agcaccccgg cgacgtcggc ggcgcgcagc
     4621 tccccggccg cgcgcagcat ctccaatgcc gaggacggca ggcgggtggt gacgacgaag
     4681 agcgccatgc gcgcccgacg ctaacgccct cgccacccgc ctgccgagat cctcagcccg
     4741 ccgcctccag cggcacccgc gccagcagcc gcgccacccc ttcgtcggag cggacctgga
     4801 gctgcacgcg cagggtcgcc cgacccgcgc cgagggcgcg cagcgctgcc ggcgccagcc
     4861 gcgcccgcac cgtcgcactc gccccggccg cgatctggcg cggcgcgtag acggcggccc
     4921 agtaagtgcg gccgccgatc cggaccggcg cgttgcgcgg cgccttgacc acgcaggggc
     4981 tggcaccggg gcagctcagc gtcgccacct tggcgcggcg cttgccgccg acctcctgca
     5041 cgtccttgat cgcaccgatc gagggcggca ccggaaccgg cggagctttc ggctcggggc
     5101 ctcccccgcc gcctttgcct ttgtcgtccg ggccgtcgtc gaccgccggc ggatcggcgc
     5161 cggcgaagtc gaccgcgaac ggcagcggcg ccttcagcgg atcggcagcg ccgcccgagg
     5221 agtaccagta ggagtagagg ccggtttcga cgtggtagtc gacgaacggc tgcggccagg
     5281 cgccccagcc ggccccgctg cggtcctgcg gggttttgcc cggtggcacg gtcacttcga
     5341 cccccgcgta gtcgggggtc acggtcagcg tgcccgccgg atcgacttcg accccggaga
     5401 aggtcgccac ggtcacgtcg gcgaccggcg gcagcgggtt gcattcgctc gggttggcca
     5461 tgctcgacgc gcagccgacc aggtcggcgc tgagcacgcc ctcgccttcg tcgtcgaccg
     5521 tcaaaaccgg gtcgctgacc gtgaacgggg cgaggccgcc gtagaagttg acgctgaagg
     5581 cgccgtccca ctcgacctcg gccgagccgt cctcctcgac ccggccgctg ccgccgtaga
     5641 gccgcaccag ctgggagccg ccgttgccga cgtggtcttg ccgggtggcc caggtcgcga
     5701 tcgcctcggc tccgctcgcc gccacctgga agacgaaggc gttgccctca tgtccgtgga
     5761 agctcgcttc gtcgccgtca gagaccccgg ccgagaagta gttgctgccg ccgaacggcg
     5821 gcgccttctg cagctcggcg ctgccggacc agtcgagggt gaccttgctg aggtcgacac
     5881 cgccgtcggg gatcgtcact ttcggcgtca ccgtcagctt cgccgcttct gaggtcgcgg
     5941 tcccgagcgg gttggccaag cgggcgcgga agatcgcccc ggactggtcg gggttggcgc
     6001 cgagcacggt cagcgagttg ccgtcgacga cgaggctctc gtcctcggcg gtgacgttct
     6061 cccagaaccc gttgacccgg cgctgccagg tcaccgtcgg ttccggtttg ccgtcggcga
     6121 gcacggcgaa ttcggcgtcg gccccctcca gcactttcgc gttgggcggg ctctgcagga
     6181 ccagcggcgc gtagtcgacg ctgagcgcag ccgtgtcgct gacgactttg cccgccgcgt
     6241 tcgagtagac ggcccgatat tcggacccgt cgtcgccgcg ggtcgcggcg acgctcagcg
     6301 tctccccaac cgcgcccggg acgtcgacga atttgctcga gcccggcgcc ttcacctgcc
     6361 actggcgggc cggcgccggg tcaccggtcg cggtcgagct gaacgtcgct tcttccgacg
     6421 cctcaccgct cgacaacgcg accgcgtcgg gctgcggctg cccgctcacc gtcggcacga
     6481 acccgagccg ttcgaatttc ccgaagcccc aggttttcga gccgccgctt tcgcccggcg
     6541 agccgtcgtt ggtctgggcg tagacgaagt gtccgtcgcc gaccagaacc gggccgccgc
     6601 gcgggtggcg ctcctggaag aactgaccgc cggcgaaggc gttacccctg accgccgcca
     6661 gcttctgcga ggtcaccccg ccgaaccaga tcgtcccgtc ggcgggatcg agcgcgtagc
     6721 gccgctcgtc gtaggggccg gcctcgacgt tgtcgcggat cgagaccgga tcgtcgcaac
     6781 cgaccccgcc ctgcccgtcg agctggcagt gcacgacctc gccgccggcc gccatccgga
     6841 ccaggacgat gtggccgttg gcgctcgtct gcacggtcga gaaggtgccc ccggcaagga
     6901 tcgtgttgag cacctcgacg cccggcagcg gctgcaccga gaccgcgtcg tcggcctggt
     6961 tgaagacgat ccggtaggcg ccggcgattt tctcgctcgc cgccagcccc gaacgggtcg
     7021 aggcgagcgc gatcagtgag ccgtccgagg cgacggcgta gttcgccggg ttgaggctgg
     7081 tggtcgggaa gccggtctgg ttctggccgg ccgggaagct gggaagtttg aacgactttt
     7141 tggtccagac gccgttctcg ccttcggcgt aggcaaacag cgtccattcg tggttgtcgt
     7201 attcgttcgc gggcacaccg gcgggaacga ttcgctgcac gcgatagccg cggttgcgga
     7261 tcgggtccca gacgaggcgc tggctgttcg cgccgatgaa cgcggtttcg ctcatctggg
     7321 ggtcaccgag caggccggtt tcgtactgct cggtctccag gttgaacttg aaccagcggg
     7381 gcatcccgct ctcgccgttg gcccggaaga gcagtctgtc cttgctcgtg tcgtaggcgg
     7441 tgacgttggt cgcccacgcc ggcgctccgc tggtcgggag gacgagggga tcgccaaccg
     7501 gctccatctc cttcaggtcg aaagcctcga tccgcacctg cgagcccggg aggctcagcg
     7561 tcgagtgcac gatcaggttc ttgcgatcga cccagagcgg ctgcaggtcg accacgtcct
     7621 cgccgtcgat gaagagcgag ttctcgacca gtttcagcac cgccgcgcca ggctggtccc
     7681 agtgctcgct gaagtccgga gcgccgcccg gaccggtgta ggagaagccg accgcgtcga
     7741 ccacctggcc gacccggtag ttgttcgcca gcgcggcgct gccctcatcg gtcacggtcg
     7801 ccgtgatccc gtcccatttc gtctccccgc cggcgaccgc cggcgtcacc ccgagcacgt
     7861 cgaggttgac gatcgggacc cgtccgtagg agatcagctc ccaggtgtcc tggttgcggc
     7921 tggtggcgac cgcggtcagc accgaggagt cgcggtcgat cgtgatctgc gggtcggcga
     7981 gcgtcacgtc gagcaggtat gggtcggtct cggggccgct gtagcccggc ggtttcggcc
     8041 ggcccagatc ggaggctttg tacttctgcc agtgggcgct gccgctgtag ctcacccgcg
     8101 tggtctgcgt cgccggatcg taggagcccg actcgaactc ccactcgagg tcgtaggccg
     8161 cgttcccggc cgcggcgacg acggcggcgc ccccgctcac ctgaggtttc tgggcgtagt
     8221 tgcgccaggt ctccttgaag ccccagttga gcgccgggcc ctcggtgatc tccaccgccg
     8281 gtt
//
LOCUS       DASRIG010000065         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1341850, whole genome shotgun sequence.
ACCESSION   DASRIG010000065 DASRIG010000000
VERSION     DASRIG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341850"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFB52_01800"
     CDS             <1..1059
                     /locus_tag="VFB52_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05093.1"
                     /translation="DSGDDDTTSVNAARKARLAKLERDGTFFSADSPWNTTVATLPVS
                     ADSEQMLRLARKRQAVIELPGEEGVDTVERTVKKGFQINAERWAPLVVEAGGNNVVST
                     KMVCRQSECGPQAERVPATLALPPETRPDPRYDGWLSVIDRENGIGYDFWRARRQSDD
                     TISYQYAKTWKLDGPGFSRPVSEDPLRAAGARGSGLPLFAGVIGPAELQKGEINHALA
                     LSVPGLARRNYVQPASVTDGVGPIDSLPAGARIRLKDTALLDDPPGEMRPSVEAILVA
                     LRSYGAIIVDRAAVPTLYAAQGTPPNLLREDELDWLHLDDFEVVELPQLRQDPPLGQV
                     QQYGLASTGAPRPVGSGG"
     gene            1056..2483
                     /locus_tag="VFB52_01805"
     CDS             1056..2483
                     /locus_tag="VFB52_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05094.1"
                     /translation="MIALRRILPALALLVALVALAGCGVSLDGAAQNGEAGPLERTTA
                     SAGDSDKNDVNLPPRPTGTVRIEGKAQGSLTETLLDSYEARGSSVNFEITNSSEKAAF
                     EAFCEGEADIVDSARPISPTEYAACQANGVEPVQIEVASDAAVLAIANETDVGVDCLS
                     VGDVREIFRAASPISSWSQVGWGGRSDPNVDALPIKVAGPDARSNVFGFFGEFVLGDP
                     EPSPLLLRGDYEAFPNDEGVRLAVSGDREQLERAAQSQSSRQVAADLANSLEDAEDAV
                     EEAEFQVDKGIEDNRTDTQQNRDSEALAQAKAKADRLRTELGEAQESAEVDDVAAAEV
                     AQRLGTLGLFRFSYYELWEERLRPMEIQITNFEGQPECIFPSQSTVTDATYPLARQLL
                     LTVDLSTMKEAEINEFLSFAVAESQEAAIETALVPLPDEVMNTELAWLSGEVAPDVVY
                     YPPGQIVRDEKEAAGETVAADEESF"
     gene            2480..>2991
                     /locus_tag="VFB52_01810"
     CDS             2480..>2991
                     /locus_tag="VFB52_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05095.1"
                     /translation="MTQAARAARLALAIVALGLLLCIPSADLAAAKAKPGLPLSATPV
                     STRTGDNVGALKLVLRPIKRAKLGPVRIVLRNSKGKLFAQQKFGATTEQRIVSLRLKR
                     PLRAGKYRVRMVGRKAKGDKLLSATRKLTFAEGGGWAGAEVVGGTLIQRVVVDWSDGK
                     WGGRDLGGFIA"
BASE COUNT          508 a         1119 c          986 g          378 t
ORIGIN      
        1 gactccggcg atgacgacac cacctcggtc aacgcggcgc gcaaagcgcg gctggcgaaa
       61 ctggagcgcg acggcacctt cttctccgcc gacagcccct ggaacacgac ggtcgcgacg
      121 ctgccggtct cggccgactc cgaacagatg ctgaggctgg cgcgcaagcg ccaggcggtg
      181 atcgagctgc ccggcgaaga gggcgtcgac acggtcgaac ggaccgtcaa aaagggcttc
      241 cagatcaacg ccgaacgctg ggcgccgctg gtcgtcgaag ccggcggcaa caacgtcgtc
      301 agcaccaaga tggtctgccg ccagagcgaa tgcggcccgc aggccgaacg cgtgccggcg
      361 accctggccc tgcctccgga gacgcggccg gacccgcgct acgacggttg gctcagcgtg
      421 atcgaccgtg agaacgggat cggctacgac ttctggcgcg cccgtcgcca gtccgacgac
      481 acgatctcct accaatacgc caagacctgg aaactcgacg gccccggctt cagccggccg
      541 gtctccgaag acccgctgcg cgccgccggc gcccgcggct cgggcctgcc gctgttcgcc
      601 ggcgtgatcg gcccggccga actccagaag ggcgaaatca accacgccct ggcgctctcg
      661 gtgcccggcc tggcgcgccg caactacgtc cagcccgcct cggtcaccga cggcgtcggc
      721 ccgatcgact cgctgcccgc gggcgcccgg atccggctca aagacacggc gctgctcgac
      781 gacccgcccg gtgaaatgcg cccctcggtc gaagcgatcc tggtcgccct gcgcagctac
      841 ggcgcgatca tcgtcgaccg cgccgcggtc ccgaccctct acgccgccca gggcacgccg
      901 ccgaacctgc tgcgcgaaga cgagctcgac tggctgcacc tcgacgactt cgaagtggtc
      961 gagctgccgc agctgcgcca ggacccgccg ctgggccagg tccagcagta cggattggca
     1021 tcgacgggcg ccccgcgccc agttgggagc ggaggatgat cgccctgcgt cggattctgc
     1081 ccgcgctcgc gctgctcgtc gccctggtcg ccttggccgg ctgcggggta agcctcgacg
     1141 gcgccgccca gaacggcgag gccggcccgc tcgaacggac caccgccagc gccggcgaca
     1201 gcgacaagaa cgacgtcaac ctgccgccgc gcccgaccgg cacggtccgg atcgagggca
     1261 aagcgcaggg ctcgctgacc gagacgctgc tcgactccta cgaggcgcgt ggctccagcg
     1321 tcaacttcga aatcaccaac agcagcgaaa aggcggcctt cgaagccttc tgcgagggcg
     1381 aggccgacat cgtcgactcc gcccggccga tctcgccgac cgagtacgcg gcctgccagg
     1441 ccaacggggt cgaaccggta cagatcgagg ttgcctccga cgccgccgtg ctcgcgatcg
     1501 ccaacgaaac cgacgtcggc gtcgactgcc tcagcgtcgg cgacgtgcgc gaaatcttcc
     1561 gcgccgcctc gccgatctcg agctggtcgc aggtcggctg gggcgggcgc tcggacccga
     1621 acgtcgacgc gctgccgatc aaggtcgccg gccccgacgc gcgctccaac gtgttcggct
     1681 tcttcggcga gttcgtgctc ggcgaccccg aaccctcgcc gctcctgctg cgcggcgact
     1741 acgaggcctt ccccaacgac gaaggcgtgc gcctcgccgt ctccggcgac cgcgagcagc
     1801 tcgaaagggc cgcccagagc cagtccagcc gccaggtcgc cgccgacctc gcgaactcgc
     1861 tcgaagacgc cgaagacgcg gtcgaggagg ccgaattcca ggtcgacaag gggatcgaag
     1921 acaaccgcac cgacacccag cagaaccgcg actccgaagc gctcgcccag gccaaagcga
     1981 aggccgaccg cctgcgcacc gaactcggcg aagcccagga atccgctgag gtcgacgacg
     2041 tcgccgccgc cgaagtcgcg cagcggctcg gaactctcgg cctcttccgg ttttcctact
     2101 acgagctctg ggaggagcgc ctgcgcccga tggagatcca gatcaccaac ttcgaaggcc
     2161 agcccgagtg catcttcccc tcgcagtcga cggtcaccga cgcgacctac ccgctggccc
     2221 gccagctgct gctgacggtc gacctgagca cgatgaagga agccgaaatc aacgaattcc
     2281 tctccttcgc cgtcgccgag tcccaggaag cggcgatcga gaccgcgctg gtcccgctgc
     2341 ccgacgaagt gatgaacacc gagctcgcct ggctcagcgg cgaggtcgcg cccgacgtcg
     2401 tctactaccc gccgggccag atcgtcaggg acgaaaaaga ggccgccggc gaaaccgtcg
     2461 ccgcggacga ggagagcttt tgacccaggc cgcccgcgcg gcgcgcctcg ccctcgcgat
     2521 cgtcgcgctg ggcctgctcc tctgcatccc cagcgctgac ctcgccgccg ccaaggcgaa
     2581 gccgggcctg ccgctcagcg cgaccccggt cagcacccgc accggcgaca acgtcggcgc
     2641 cctgaagctg gtcctgcggc cgatcaagcg ggcgaagctg ggccccgtgc ggatcgtcct
     2701 gcgcaactcg aagggcaagc tgttcgcgca gcagaagttc ggcgcgacca ccgaacagcg
     2761 gatcgtctcg ctgcggctga agcggccgct gcgcgcgggc aagtaccggg tgcggatggt
     2821 cggccgcaag gccaagggcg acaagctgct cagcgccacc cgcaagctca ccttcgccga
     2881 aggcggtggc tgggccggcg ccgaagtcgt cggcggcacc ctgatccagc gggtcgtggt
     2941 cgactggtcc gacggcaagt gggggggacg cgacctcggc ggcttcattg c
//
LOCUS       DASRIG010000066         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2116542, whole genome shotgun sequence.
ACCESSION   DASRIG010000066 DASRIG010000000
VERSION     DASRIG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116542"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..725
                     /locus_tag="VFB52_01815"
     CDS             102..725
                     /locus_tag="VFB52_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05096.1"
                     /translation="MAAVAVAGYAIGAGGSSKNLVLCAEKKDGDLALASAKGKCAKGE
                     KKLTVAKEGPVGPQGAPGPAGADANATLEAVHYVTGPPNPKCFEQAATFCKGESLSGG
                     WRNLSQYVSGYEDVGFYKEANGTVHLVGTAYYSSSGGESGGFEPEGPFYLPPGFRPAK
                     TLGFTAPGAGGQSLAGTAPVLIRPNGAVAILSPVESVALDGISFRTP"
     gene            729..1388
                     /locus_tag="VFB52_01820"
     CDS             729..1388
                     /locus_tag="VFB52_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05097.1"
                     /translation="MTRRWTVLALLAGVAVIAIGAYAIGAGKSSKDLVLCAAKKGGDL
                     SLATAKGKCAKGEKKLTVAKEGPVGPAGPQGAPGSAANVASEPVTYVTGPANDQCFEK
                     PGTFCESESGYVTWGNYSPEFGNVGYYKDAAGVVHLTGVASSAVGGVVFGFQPEGPFY
                     LPPGYRPSVVKMFSVPSQTAPEEEFSDHVTIRVRPNGVVAISTDDNVREVALEGVAFV
                     P"
     gene            1447..2346
                     /gene="folP"
                     /locus_tag="VFB52_01825"
     CDS             1447..2346
                     /gene="folP"
                     /locus_tag="VFB52_01825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZO05098.1"
                     /translation="MTGSPSWRLSDRTLDLAQPLGAAIVNVTVDSMFEGARSGTPEQA
                     VRDGQALAEAGFDMLDVGAVAARSGPPVAPEDEAAALVPAIEGLAETLHLSPSGLQMQ
                     GGVPVSADTFSVEVARAALDAGAQAVNDISGGNEELFALVAERGCGYVLMHIEGPPRV
                     DRPWRDYGDVVDHLRAWFEQRVELAREQGVAEEQIAIDPGLDFDLSPEQDLEILRRLG
                     ELRALGLPLYVSLSRKDFIGAVLAGSWEERWGPSEREWGTVAAVALAVREGADILRIH
                     DRSSLQAMRISGRIRGSAARSAA"
     gene            2346..>2711
                     /locus_tag="VFB52_01830"
     CDS             2346..>2711
                     /locus_tag="VFB52_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO05099.1"
                     /translation="MARGGNLLEHSWSVAIDPARRDGRIVAETSEDERRARPVGLPAE
                     LDAGLVAALERAGIERLYSHQLAAYEAAADSNLVITSGTASGKSLAFNLPVLDGIAAD
                     PKRRALYLYPTKALAQDQAR"
BASE COUNT          410 a          916 c          974 g          411 t
ORIGIN      
        1 gtggaaagca cccagttttc tgtacagatc ggtttcccat tccaatcagg tctgtagggt
       61 gcgccgggat gcgttggggg atcggagtac ttctcgtcat ggtggctgcg gttgcagttg
      121 ccggctacgc aatcggggca ggtggctcgt ccaagaacct cgtgctctgc gctgagaaga
      181 aagacggcga cctcgcgctg gcgagcgcca aaggcaagtg cgcgaaaggc gagaagaagc
      241 tgacggtggc gaaggaaggc ccggtcgggc cacagggcgc tccggggcct gcgggggccg
      301 atgcgaacgc gacgctggaa gcggtccact acgtgaccgg gccgccgaac cccaaatgct
      361 tcgaacaggc cgccaccttc tgtaaggggg agagcctgag cggcggctgg cggaacctct
      421 ctcaatacgt ctccggctac gaagacgtcg gcttctacaa agaggcgaac ggcaccgtcc
      481 acctcgttgg caccgcttac tactcctcca gcggcggtga gtctggcggc ttcgaacctg
      541 aaggcccgtt ctacctgccg cccgggttcc gccccgcgaa gaccctgggc ttcacggctc
      601 ccggcgccgg cggccagtcg ctggccggca ccgccccggt cctcattcgc cccaacggcg
      661 cggttgcgat cctcagcccc gttgaatccg tcgccctcga cggaatctcc ttcaggacgc
      721 cctgaccgat gacccgtcgc tggaccgtcc tcgctctgct cgccggtgtt gcggtgatcg
      781 cgatcggtgc ctacgcgatc ggcgccggca aatcctccaa ggacctcgtg ctctgcgccg
      841 cgaaaaaagg cggcgacctc tcgctcgcga ccgccaaagg caagtgcgcg aaaggcgaga
      901 agaagctgac ggtggccaag gaagggccgg tcggcccggc tgggccgcag ggtgcgccgg
      961 gatcggcggc caacgtcgcc tcggagccgg tcacctacgt gaccggtccg gccaacgacc
     1021 agtgcttcga aaaaccgggg acgttctgcg aatcggagtc gggctatgtc acgtggggga
     1081 actactcccc ggaattcggg aacgtcgggt actacaaaga cgccgccggg gtcgtgcacc
     1141 tgaccggagt cgcgagctcc gcggtcggcg gggtggtctt cggcttccag cccgaaggcc
     1201 cgttctatct cccgcccggg taccggccga gcgtcgtgaa gatgttcagc gttccctcgc
     1261 agacggcccc cgaagaggaa ttcagcgatc acgtcaccat cagggtccgc ccgaacgggg
     1321 tggtcgcgat cagcaccgac gacaacgtcc gcgaggttgc cctcgaaggc gtcgcgttcg
     1381 tcccgtaacg cgggctctcc ggcggtgccc gcggagcggg ccggcctcgt cagctagaaa
     1441 gggggagtga ccggctctcc ttcatggcgt ctctccgacc ggaccctcga cctggcgcag
     1501 ccgcttggcg cggcgatcgt caacgtcacg gtcgactcga tgttcgaggg cgctcgcagc
     1561 ggcacccccg agcaggcggt gcgcgacggg caggcgctgg ccgaggccgg cttcgacatg
     1621 ctcgacgtcg gcgcggtcgc ggcgcgcagc ggaccgccgg tcgcgcccga ggacgaggcg
     1681 gcggccctgg tcccggcgat cgaagggctc gctgaaaccc tgcatttgtc gccctctggg
     1741 ctacaaatgc agggcggggt gccggtgtcg gcggacacgt tttcggttga ggtggcgcgg
     1801 gcggcgctcg acgcgggggc gcaggcggtc aacgacatct ccggcggcaa cgaggagctg
     1861 ttcgcgctgg tcgccgagcg cggctgcggc tacgtgctga tgcacatcga ggggcctccc
     1921 cgggtcgacc ggccgtggcg tgattacggc gacgtcgtcg accatctgcg cgcgtggttc
     1981 gagcagcggg tcgagctggc gcgggaacag ggcgtcgccg aggagcagat cgcgatcgac
     2041 cccggactcg acttcgacct cagccccgag caggacctgg agatcctgcg gcggctcggc
     2101 gagctgcggg cgctggggct gccgctctac gtctcgctct cgcgcaagga cttcatcggc
     2161 gcggtgctcg ccggctcctg ggaggagcgc tggggcccga gcgagcgcga gtggggcacg
     2221 gtcgcggcgg tggcactggc ggtccgcgag ggcgctgaca tcctccgcat ccacgaccgt
     2281 tcttcgctgc aggcgatgcg gatttctgga cggatccgcg gctcggccgc ccggagcgcc
     2341 gcctgatggc gcgcggcggc aacctgctcg agcactcctg gtcggtcgcg atcgacccag
     2401 cccgccgcga cgggcggatc gtcgccgaga cctccgagga cgagcgccgg gcgcggccgg
     2461 tcgggttgcc ggcggagctc gacgccgggc tcgtcgccgc gctcgagcgg gctgggatcg
     2521 agcggctcta ttcgcaccag ctcgccgcct acgaggcggc cgcggactcg aacctggtga
     2581 tcaccagcgg cacggcctcg ggcaagagcc tcgccttcaa cctgccggtg ctcgacggga
     2641 tcgccgctga cccgaagcgc cgcgcgctgt atctctatcc gacgaaggcg ctggcccagg
     2701 accaggcgcg c
//
LOCUS       DASRIG010000067         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1086085, whole genome shotgun sequence.
ACCESSION   DASRIG010000067 DASRIG010000000
VERSION     DASRIG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086085"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..557)
                     /locus_tag="VFB52_01835"
     CDS             complement(123..557)
                     /locus_tag="VFB52_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZO05100.1"
                     /translation="MAFVPPARHLLRARDLADARYAEPIDVADMAAAAGLSRAHFSRE
                     FKRTFGESPYVYLLTRRLERATALLRNTDYSVAEICLDVGLTSVGSFTTTFKRIYGQT
                     PTEYREAHPPASAHAVVPGCFVRTYGRPQHRTFREDSAAAQT"
     gene            583..2406
                     /locus_tag="VFB52_01840"
     CDS             583..2406
                     /locus_tag="VFB52_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05101.1"
                     /translation="MVGTYAAVLAICGASLAFGQAALALCGMRRWSWLAPPVGLALLA
                     ALCWATVRLPGEGVIPAILVPLLLIAAVGFLRGRVEGGGEALRVGWPVALGALVAASL
                     PFIAEGHFGILGTSFNPDMSQHLLATDRLAEGHGSQLLTQGYPLGPHAIVVALDKGLG
                     VGLVQGFSGLTVAVAVLAPLTALAAFAELGGRGWFPALVRTAAALVVGLAYMVASSFA
                     QGAFKETMEALFLLGFVLALREATRSWNALPLRFVPAALIAVGAVYAYSFPGLIWLGG
                     AFVFWLFAEALLGRRPPGWRPLGLAALVFAVLIAPEVGRMIDFKNFETFDPNGPGLGN
                     LFGQVSPFEALGIWPSGDFRIAPGDGAVPAIGYYLGAAFALALLVYGIWRCARNREVA
                     ILSGLAAAALAYAAGRIGGTAYASSKAIEIAAPLCALVILLPLLRESANAGAPGMREK
                     LRSEAGRERGSLLAGAAAVVFIAAAALCSLLAFANAPVGPTSYSPALTGLRPLVANDS
                     VVYVASEELLADEHGTPYVSWELRGGRVCIETAAEADGAPPPGVRFVITEASRDDPPF
                     PGLKPVRIALPYVLWEVTGPVTGKSPCPLIAVRQARQGPAR"
     gene            2427..3920
                     /gene="thrS"
                     /locus_tag="VFB52_01845"
     CDS             2427..3920
                     /gene="thrS"
                     /locus_tag="VFB52_01845"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZO05102.1"
                     /translation="MEAATDKFVVTLPDGTPLELPVGATGADAAAAIGAGLAKAALAI
                     KVDGELRDLAAPLPEGGAEIAILTSRDPEALELIRHDAAHVMAEAVMDLYPGTKVTIG
                     PPIESGFYYDFEFPPGTKITEEDLPKIEQAMLAHIKADEEFDRRDVPVTEAIELFRSQ
                     NQDFKVELIEDLVKNEGVETVSLYRNGPFEDLCRGPHGPSTGRIKAIKLNSVAGAYWR
                     GDETRESLTRIYGTAFFSKADLEEYLERLEEAKARDHRRLGPQLGLFMLRKEAPGMPF
                     WLPNGTTLLHLIEAEVQKQLTKRGYEEIKTPHVMDVELWHRSGHYDNYRENMYFSQWG
                     DREFAIRPMNCPGACLVFGSDRHSYRELPLRLAEFGQVSRAEREGVLHGLLRVRAFTQ
                     DDAHVFCSMDQVTDEVDSICEAIDELYARFGFEDVRVELSTRPEKSIGTDEQWQRAEE
                     SLRAAPGGSSPAGRARRRRRCSRARSARASGSRVGRCRTARSGRARR"
     gene            3922..>4348
                     /locus_tag="VFB52_01850"
     CDS             3922..>4348
                     /locus_tag="VFB52_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05103.1"
                     /translation="MEVDLRPVEGAVAGVQLVLAALAVERLFQRRLGLRPLLLGPHRL
                     LGPGRELDFDVLEAKAGVELVDRLADRGDLVGDLVLGAVDVGVVLGECAHPQQPVQHT
                     LALVSADAAELGQAQRQLAVGVAVGGVDETGAGTVHRPQR"
BASE COUNT          583 a         1534 c         1512 g          719 t
ORIGIN      
        1 cctccatccc gaccttctcg gtccagaagg cgagcgcctc gtcctggtcg tggacccaga
       61 gctgtgcgtt tgcgacctgc atttcgtctc tccttggttc gaatttcgag cttgcggcga
      121 gcctaggtct gcgccgcggc gctgtcttct cgaaacgtgc ggtgttgggg gcgtccgtag
      181 gtgcggacga agcagccggg cacgaccgcg tgggcggagg cgggcggatg ggcctcgcga
      241 tattcggtcg gggtctgccc gtagatccgc ttgaaggtgg tggtgaagga gccgacgctg
      301 gtcagcccga catcgaggca gatctcggcg accgagtagt cggtgttgcg aagcagcgcc
      361 gtcgcccgct ccaggcggcg ggtcagcagg tagacgtagg gcgactcgcc gaaggtgcgc
      421 ttgaactcgc ggctgaagtg ggcgcgcgag aggcccgccg cggcggccat gtcggcgacg
      481 tcgatcggct ccgcgtagcg ggcgtcagcg aggtccctgg cgcgcagcaa atgcctggct
      541 ggcggaacga acgccatctc cgcagtctag gctcgcggct tcgtggtcgg gacctatgca
      601 gctgtactgg cgatctgcgg cgcctcgctg gcgttcgggc aggcggcgct cgcgctctgc
      661 gggatgcgcc gctggtcgtg gctggcgccg ccggtcggcc ttgccctgct ggcggcgctc
      721 tgctgggcga ccgtgcggct gcccggcgaa ggcgtcatcc cggcgatcct cgttccgctg
      781 ctcctgatcg ccgcggtcgg cttcctgcgc ggccgggtcg agggcggggg agaggcgctt
      841 cgcgtcggct ggccggtcgc cctcggcgcc ctggttgccg cctcgctgcc gttcatcgcc
      901 gagggccact tcgggatcct cgggaccagc ttcaaccccg acatgtccca gcacctgctg
      961 gcgaccgacc ggctcgctga gggccatggc tcgcagctgc tcacccaggg ctatccgctc
     1021 ggcccgcacg cgatcgtcgt cgccctcgac aaggggctcg gggtcggcct ggtccagggc
     1081 ttcagcgggc tcaccgtggc ggtcgcggtg ctcgcgccgc tgaccgcact ggcggccttc
     1141 gccgagcttg gcgggcgcgg ctggttcccg gcacttgtcc gcaccgccgc ggccctggtc
     1201 gtcggactcg cctacatggt cgcctcctcc ttcgcccagg gcgccttcaa ggagaccatg
     1261 gaggcgctct tcctgctcgg cttcgtcctg gcgttgcgcg aggcgacgcg gagttggaat
     1321 gcgctgccgc tgcgctttgt tccggcggcg ctgatcgccg tcggcgccgt ctacgcctac
     1381 agcttccccg gcctgatctg gctcggcggc gcgtttgtct tctggttgtt cgccgaagca
     1441 ctgcttggcc gtcgcccgcc cggctggcgg ccgctggggc tggcggcgct cgtcttcgcg
     1501 gtgctgatcg cgcccgaggt cggccggatg atcgacttta agaacttcga gaccttcgac
     1561 cccaacggcc ctgggctcgg caacctcttc ggccaggtct cgcccttcga ggcgctcggg
     1621 atctggccct ccggcgactt ccgcatcgcc cccggcgacg gcgccgtgcc ggcgatcggc
     1681 tactacctcg gcgccgcctt cgccctcgcg ctgctcgtct acggcatctg gcgctgcgct
     1741 cgcaaccgcg aggtggcgat cctctccggc ctcgccgccg ccgccctcgc ctacgccgcc
     1801 ggccgcatcg gcggcaccgc ctacgcgtcg tccaaggcga tcgagatcgc cgcgcccctg
     1861 tgtgccctcg tcatcctcct gccgctgctg cgtgagagcg cgaacgcagg agctcccggt
     1921 atgcgggaaa aactgcgttc ggaggccggg cgggaacgag gttcgctgct cgccggcgcg
     1981 gcggccgtcg tcttcattgc cgccgcggcg ctttgctcgc tactcgcatt tgcgaatgcg
     2041 cctgtggggc cgactagcta ctcaccggcg ctgaccgggc tgcgcccgtt ggtcgcaaac
     2101 gactcggtcg tctacgtcgc ctccgaagag ttgctcgcgg acgaacacgg caccccgtat
     2161 gtctcctggg agctgcgcgg gggacgggtc tgcatcgaga ccgccgccga agccgacggg
     2221 gcgccaccgc ccggcgtccg ctttgtgatc accgaggcca gccgggacga tccgccgttc
     2281 ccgggcctga agccggttcg catcgccctg ccctacgtgc tctgggaagt caccggcccg
     2341 gtcacgggca agagcccgtg tccgctgatc gccgtgcgcc aggcccgcca gggccccgct
     2401 cgctgagcgc cattaccatc cccggaatgg aggccgctac ggacaagttc gtcgtcaccc
     2461 tgcccgacgg caccccgctg gagctgccgg tgggggcgac cggcgctgac gcggcggcgg
     2521 cgatcggcgc cggcctggcg aaggcggcgc tggcgatcaa ggtcgacggc gagctgcgcg
     2581 acctcgccgc gccgctgccc gagggcggtg ccgagatcgc gatcctcact agccgcgacc
     2641 ctgaggcgct ggagctgatc cgccacgacg ccgcgcacgt gatggcggag gcggtgatgg
     2701 acctctaccc gggcaccaag gtgacgatcg gcccgccgat cgagtccggc ttctactacg
     2761 acttcgagtt cccgcccggt acaaagatca ccgaagagga cctgccgaag atcgagcagg
     2821 cgatgctcgc tcacatcaag gccgacgagg agttcgaccg tcgcgacgtc ccggtcaccg
     2881 aggcgatcga gctcttccgc tcccagaatc aggacttcaa ggtcgagctg atcgaggacc
     2941 tggtcaagaa cgaaggggtg gagacggtct cgctctaccg caacggcccc ttcgaggacc
     3001 tctgccgcgg cccgcacggc ccttcgaccg gccggatcaa ggcgatcaag ctgaactcgg
     3061 tcgctggcgc ctactggcgc ggcgacgaga cccgcgagag cctcacccgg atctacggca
     3121 ccgcgttctt ctccaaggcc gacctcgagg agtacctgga gcggctggag gaagccaagg
     3181 cgcgcgacca ccgccgcctc ggtccccagc tcggcctctt catgctgcgc aaggaggccc
     3241 cggggatgcc gttctggctg cccaacggga ccacgctgct gcacctgatc gaggccgagg
     3301 tccagaagca gctgaccaag cgcggctacg aggagatcaa gacgccgcac gtgatggacg
     3361 tcgaactctg gcaccgctcc ggccattacg acaactaccg cgagaacatg tacttctccc
     3421 agtggggcga tcgcgagttc gcgatccggc cgatgaactg ccccggcgcc tgcctcgtct
     3481 tcggctccga ccgccactcc taccgcgagc tgccgctgcg cctcgccgag ttcggccagg
     3541 tctcgcgcgc cgagcgcgag ggcgtcctgc acggcctgct gcgggtgcgc gccttcactc
     3601 aggacgacgc ccacgtcttc tgctcgatgg accaggtcac cgacgaggtg gactcgatct
     3661 gcgaggcgat cgacgagctc tacgcgcgct tcggcttcga ggacgtccgc gtcgagctct
     3721 cgacccggcc ggagaagtcg atcgggacag acgagcagtg gcagcgcgcc gaggagagtc
     3781 tccgagcagc gcccggtgga tcatcaccgg ccggtagggc gcgtcgtcgg cgccggtgta
     3841 gtagagctcg aagcgctcgg gcatctggaa gtcgagttgg caggtgccgc actgccagga
     3901 gcggccgagc gcgtcggtga cgtggaagtc gatcttcggc ccgtagaagg cgccgtcgcc
     3961 ggggttcagc tcgtactcgc ggccctggcg gtcgagcgcc tctttcagcg ccgcctcggc
     4021 cttcgcccac tgctcctcgg tccccatcga cttctcgggc cgggtcgaga gctcgacttt
     4081 gacgtcctcg aagccaaagc gggcgtagag ctcgtcgatc gcctcgcaga tcgaggcgac
     4141 ctcgtcggcg atctggtcct cggtgcagta gacgtgggcg tcgtcctggg tgaatgcgcg
     4201 cacccgcagc agcccgtgca gcacaccctc gcgctcgttt cggctgacgc ggccgaactc
     4261 ggccaggcgc agcggcagct cgcggtagga gtggcggtcg gaggagtaga cgagacaggc
     4321 gccgggacag ttcatcggcc gcagcgcg
//
LOCUS       DASRIG010000068         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1592496, whole genome shotgun sequence.
ACCESSION   DASRIG010000068 DASRIG010000000
VERSION     DASRIG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592496"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VFB52_01855"
     CDS             complement(<1..867)
                     /locus_tag="VFB52_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05104.1"
                     /translation="MGGSLNRERIALLAVWGLAALACLLGLAKPPADGLHGGKGEQLL
                     DLIRVLSTTALALTLLLGPGIVWRAARGDRNPPLAFLFLPGLAVMAATGGLAWLLAPS
                     VDPEVTSFAVSAPLLGLLLGGLLAAGRERVFEPAEWGALLIVGCVLALAVGRALWSMG
                     PEGELFSGSIARTLEVGDRSDARLSYATVQLVAHGSPPFGAAGTEYFAPYDFSSRGPV
                     PGLASSPVVFLSGGEPPLALPTGNAWEPFDAQGYQAYRLAMIVFACTAFLALWQLVRR
                     LGGETAGRLALLL"
     gene            896..2572
                     /locus_tag="VFB52_01860"
     CDS             896..2572
                     /locus_tag="VFB52_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05105.1"
                     /translation="MPAARRAPLLLLGLALAGSAVLLLLLDRNLTFFQDSWAFLMHRQ
                     GNALDDFLKPHNEHIVVIPVALQKLLLAIFGMSSSMPEFVLLTVTLLITAVLLFVYVR
                     RRLGDWVALFAAVLVLFLGPAAQVLLWPFEIALVGSTMTGLAMLLALEREDRVGDLLA
                     CLMLVLAIGFSSLGLAFAVGAGVDVLQRRRTLGLRRLYVPAVPLVLYAAWYLGWGHEA
                     ESSFSLGNVVHSPVFLIEGLGASLAALTGLSALTDSVDKRPWAGMALLAILGALLIWW
                     LWTRRPGISDRFWPVAATAATFWLLGGFNRGAGREAEASRYMHIGAILLLMMAADLLA
                     GSRLRGKRAAQAVLVGAALVIAISALNLKPLDDGYDSFRDETVLTRADLGAMQIAERT
                     IPPTFSLTEEIAGTPSLIDVNATEYFPAEEDHGSPAYTPEELASAPAAGRKQADIVLA
                     QALPLSTAVAEDAALPPAILPGCERAGAAVEEVRLRPGLTQIGVPPGPEANFSLRRFA
                     TGEFPVVTAGAPGDSVTTLKIPRDTAPQPWHLHVDAPQGAFVCRARSTGA"
     gene            complement(2569..>3078)
                     /locus_tag="VFB52_01865"
     CDS             complement(2569..>3078)
                     /locus_tag="VFB52_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05106.1"
                     /translation="TQFPRLVIPLSVVLTAFFNLLLNLIIVFIFVLAWGVTPTWTWLL
                     FPFALGLIAVFTAAVSMMLAVLYVRFRDVAIIWTVVSQVLFYATPVLYPLTVLKDQTL
                     ERLMLINPLAPIFTEVQGWVINPDEPTAANIAGGWYYLLPAVFLYIATCAFSVWIFNR
                     DAPRIAEEL"
BASE COUNT          453 a         1111 c         1095 g          419 t
ORIGIN      
        1 gagcagcagc gcgaggcgcc ccgcggtctc gccgccgagc cggcgcacca gctgccagag
       61 ggcgaggaag gcggtgcagg cgaagacgat catcgccagg cggtacgcct ggtagccctg
      121 ggcgtcgaac ggctcccagg cgttgcccgt cggcagcgcc agcggcggct cgccgccgct
      181 gaggaagacg accggcgagc tggccagccc gggcaccggt ccgcggctgg agaagtcgta
      241 gggggcgaag tattcggtgc cggcggcgcc gaagggcggc gagccgtggg cgacgagctg
      301 cacggtcgcg taggagagcc gcgcatcgga gcggtcgccg acctcgagcg tgcgcgcgat
      361 cgagccgctg aacagctcgc cttcggggcc catcgaccag agcgcccgtc cgaccgcgag
      421 cgcgagcacg caaccgacga tcagcagcgc tccccactcg gcgggctcga agacgcgctc
      481 ccggccggcg gcgagcaggc cgccgagcag cagcccgagc agcggcgcgc tgaccgcgaa
      541 gctggtcacc tcggggtcga ccgaaggggc gagcagccag gcgaggccgc cggtcgccgc
      601 catcaccgcc aggccgggga ggaagaggaa ggcgagcggg gggttgcggt cgccgcgcgc
      661 cgcccgccag acgatcccgg ggccgagcag cagggtcagc gccagggcgg tggtgctgag
      721 cacccggatc aggtcgagca gctgttcgcc cttgcccccg tgcaggccgt cggccggcgg
      781 cttggccagc ccgagcaggc aggccaatgc cgccaacccc cagacggcca gcaacgcgat
      841 ccgctcccga ttgaggctcc cgcccatgag cgcgctgaca atacactcgc gcccgatgcc
      901 ggctgcgcgg agagcaccgc tgttgctgct cgggctggca ctcgccggct cggcggtcct
      961 gctgctcctg ctcgaccgca acctcacctt cttccaggac agctgggcct tcctcatgca
     1021 ccgccagggg aatgcgctcg acgacttcct caaaccccac aacgagcaca tcgtggtgat
     1081 cccggtggcg ctgcagaagc tgctgctggc gatcttcggg atgtcctcgt cgatgcccga
     1141 attcgtgctg ctgacggtga cgctgctgat caccgccgtg ctgctcttcg tctacgtgcg
     1201 gcgccggctc ggcgactggg tggcgctgtt cgccgccgtg ctcgtgctct tcctcggccc
     1261 cgccgcgcag gtcctgctct ggcccttcga gatcgccctg gtcggctcga cgatgaccgg
     1321 gctggcgatg ctgctggcgc tcgaacgcga agatcgggtg ggggacctgc tcgcctgcct
     1381 gatgctggtg ctcgcgatcg gcttctccag tctcggcctc gccttcgcgg tcggcgccgg
     1441 ggtcgacgtc ctgcagcggc gccgcacgct cggtctgcgc cgcctctacg tccccgcggt
     1501 cccgctcgtc ctctacgccg cctggtactt gggctggggc cacgaggccg aaagcagctt
     1561 ctcgctcggc aacgtcgtcc actcgccggt cttcctgatc gaggggctgg gcgcctcgct
     1621 ggcggcgctg accgggctct cggcgctgac cgacagcgtc gacaagcgcc cctgggcggg
     1681 gatggcgctg ctcgcgatcc tgggcgcgct gttgatctgg tggctgtgga cgaggcggcc
     1741 ggggatctcg gaccgcttct ggccggtcgc tgcgaccgcg gcgaccttct ggctgctcgg
     1801 cggcttcaac cgcggtgccg ggcgcgaggc ggaggcgagc cgctacatgc acatcggcgc
     1861 gatcctgctg ctgatgatgg ccgccgacct gctcgcgggg tcgcgcctgc gcggcaagcg
     1921 cgccgcccag gcggtcctcg tcggcgccgc tctggtgatc gcgatctccg cgctcaacct
     1981 gaagccgctc gacgacggct acgacagctt ccgcgacgaa accgtgctga cccgcgccga
     2041 cctcggcgcg atgcagatcg ccgagcgcac gattccgccg accttcagcc tcaccgagga
     2101 aatcgccggg acgccctcgc tgatcgacgt caacgcaacc gagtacttcc cggccgagga
     2161 agaccacggc tcacccgcct acaccccgga ggagctggcc agcgcgccgg cggcgggccg
     2221 caagcaggcc gacatcgtgc tcgcgcaggc gctgccgctc tcgaccgcgg tcgccgagga
     2281 cgccgcgctg ccacccgcga tcctgcccgg ctgcgagcgc gccggcgccg ccgtcgagga
     2341 ggtccgcctg cgccccggcc tcacccagat cggcgtcccg cccggacccg aagccaactt
     2401 cagcctgcgc cgcttcgcca ccggcgaatt ccccgtcgtc accgccggcg cccccggcga
     2461 ctcggtcacc accctgaaga tcccccgcga caccgccccc cagccctggc acctccacgt
     2521 cgacgccccc cagggcgcct tcgtctgccg cgcccgctcg accggggcct aaagctcctc
     2581 ggcgatccgc ggcgcgtcgc ggttgaagat ccagacgctg aaggcgcagg tcgcgatgta
     2641 gaggaagacc gcggggagca ggtagtacca gccgccggcg atgttggcgg cggtcggctc
     2701 gtcggggttg atcacccagc cctggacttc ggtgaagatc ggggccaggg ggttgatcag
     2761 catcagccgt tcgagcgtct ggtctttgag cacggtcagg gggtagagga ccggggtcgc
     2821 gtagaagagc acctgcgaga ccacggtcca gatgatcgcg acgtcgcgga agcgcacgta
     2881 gaggaccgcg agcatcatcg agaccgccgc ggtgaagacc gcgatcaggc cgagcgcgaa
     2941 ggggaagagc agccaggtcc aggtcggcgt cacgccccag gcgagcacga agatgaagac
     3001 gatgatcagg ttcagcagca ggttgaagaa ggcggtgagg acgaccgaga gcgggatcac
     3061 cagacgcggg aactgggt
//
LOCUS       DASRIG010000069         8013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3156618, whole genome shotgun sequence.
ACCESSION   DASRIG010000069 DASRIG010000000
VERSION     DASRIG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156618"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFB52_01870"
     CDS             <1..678
                     /locus_tag="VFB52_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO05107.1"
                     /translation="VEIAYPGISTWKITAIGKTAHPTEPEHGINAVTKMAKLVEAVDQ
                     NRLELPKGDSQWFEPRVTTNGIRTRPGGGWAIPGRCDAVLSILSPPGASLNEVRDAVD
                     AFLRMLEQEDGEVKYERKILPMGAGRLWLRPGECDPEHAGVKALEAAVREHGQKDPEV
                     RKFNGGWVDAVELMRPGANGFSEPAVITFGPGDFELAHTVDEHIPISEVAEAAEIYAA
                     ATKQLLS"
     gene            713..2584
                     /locus_tag="VFB52_01875"
     CDS             713..2584
                     /locus_tag="VFB52_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO05108.1"
                     /translation="MKESPASGALTAFSAVAAELDRVEKIDDLLVAIARQISELVGVE
                     RCSVFLRDGREDLFRGCVSRGVDGIHDAEIRQCVAGIPADRVTREAVRTRKPVIVANA
                     RSDNRMVKSTVRRWEIRSVMWVPMVIGEETIGLITLDDLGRQHAFTEADAEAAGRFAG
                     LAAVAVAQTQRRLELSSKLAVAGRQVQSLRRATAVDEQLTDLVLARKPLEDLLGTLSE
                     LLMKPCAIFDDAGNRVVTALPEGAEESAVPRLLEPELAHSPEVRRALAEHDGSRVFVV
                     GPLPQVNLLRRHVVAPVQLGEEVWGRLVVMEHKTRFNGGDMIAVRRAAALIALQVSAD
                     RNAVEADWNAGSSLAAELLGGSSDLTTVQRRADRLGLNLEAPRVVMLLAARSGDEEEA
                     PDFRSVAAAFDAVAPQLQVQVTAVSGAAAALVELPVDGAAGAAALDFKDTFEAVRMHI
                     DPSLIGGVSGVYSTREGYRTGHREARQVTECVRRFSPPGGPALFTAEELGVGSLLLSS
                     SDSEAVSQFAEQTVGELVREHSKADLLTTLCSFFENMGSIRGSAAALDVHENTIRYRL
                     SRVEELTGLAIMHDPDAQLRARLSLLVLMLKGRLPSGLSAAPAQSEGISLEVVDAAA"
     gene            2789..3766
                     /locus_tag="VFB52_01880"
     CDS             2789..3766
                     /locus_tag="VFB52_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZO05109.1"
                     /translation="MTHDAVTAALADAGLAPRAVDSLALASGEHGATLGCQEALGHPV
                     RSTECDSTHGALALHTAWAAVATGESDVAVCVGHDPNPAGAASVLPIDAMAAAARLYM
                     RESGATEEHLARVAAKNRGHGAANPRALVREPVDAKAVLASAPMAWPLRRMMVPLPAQ
                     AAAAVVLGREDRGRRDGPRAPRLPASVLLSADEGRPAVARAAGLAYFGAALGPEDVDV
                     AEVEDLTPATEIAAYEALELAPAQRGPELVETGFTELGGVVPVNVSGGSLAQGRVPGS
                     GAILQLIELAAQLRGRAGRRQVPGARVGLALCGDSIGPRAATSLTIVGA"
     gene            3983..5512
                     /locus_tag="VFB52_01885"
     CDS             3983..5512
                     /locus_tag="VFB52_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05110.1"
                     /translation="MSRALSTDAKTLPELLRTRAAEIGEERFVRDAHQEWTYAEFAAR
                     VAEVAGGLKARGVGEGDVVGVVLPNSPHYLEVWWGILQLGATFNPVNPALTTREQVGI
                     LADSRACCVIATEDAAAGLEENRDQLPNLRELVVAGETDLAAPLRGETPVEEPASFGP
                     DQIASFVYTSGTTGRPKGAMLSHGNLLMNAWQLGEPLPVGRGDTMGMVLPLFHVNAQV
                     VTTVIPLFLGAQTAMWERFSASRFWQEVALFEPVTFSSVPTMLAALLHAPGADEAEGN
                     SLRFVICGAAPLSPALFRRFEEKFGLKIMEGYGLTEGTCCSTINPFYGPRKVGSIGLP
                     TRGQEVVIFGDDGEIAGPGTVGEVGIRGRNVMAGYYERPDANAETLRDGWLHTGDVGY
                     QDEDGYFFLVDRVKDMIIRGGENIYPREIEDVLLENANVKEAAVVGRPDEVRGEEVHA
                     VVALVEGDDVVVLEEHCRERLAAFKVPSSWEVVPELPKTSTGKIDKKPLRAKLADAAS
                     R"
     gene            5525..6289
                     /locus_tag="VFB52_01890"
     CDS             5525..6289
                     /locus_tag="VFB52_01890"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05111.1"
                     /translation="MRMLDGKSILITGAASGIGEATARKAASEGARLVLADVNDEKGE
                     AFREELGGDAIYVNCDVTSEAAVEAAVAKTVAEHGSIDGAFNCAGILHALGNTGENPL
                     DEWQKVIDVDLVGVFLCTKHEILAMAAQGSGSILNMASAAGLIGWPGASAYVAAKHGV
                     VGLTKSAALEYAEAGVRVNSICPSYVLTPMVEKDLFEDALGGDEELIAAARANHPIGR
                     FAQPEEIAEAATWLLSDRASFVTGTAMSVDGGYTAP"
     gene            6312..7001
                     /locus_tag="VFB52_01895"
     CDS             6312..7001
                     /locus_tag="VFB52_01895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZO05112.1"
                     /translation="MADRDSFEQSPYELPPGSTEVVLVRHGASEAAVVGARFPLVDGH
                     SDPALAESGKAQAEMVAEALRGEEVAGIFASTLRRTQESAAPLAEELGLEPQVLFELR
                     EVYLGDFEGGEYRIRQGRGDPIIQQVFAEERWDAIPNAESLDDFGARLKKGIEGIVAT
                     VGPETTAIAFVHGAVIGRLCQIATDSRPFAFVHADNCSISRIVVHADGKWLLRSFNDI
                     NHLTVKTGPAG"
     gene            7014..7745
                     /locus_tag="VFB52_01900"
     CDS             7014..7745
                     /locus_tag="VFB52_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO05113.1"
                     /translation="MPAFLVHGNPDSSRLWNRVKQHLGSYDGEVVAGDLPGFRDPAPE
                     GFPSTKESYVEWICEQCEALGGNVDLVGHDWGSLLVQRVASIRPDLLNTVACGGAVVD
                     VNYEWHPLAQVWQTPEEGERYMAEELTPEFGIEHLVENGVPREDAEANIWLTEYGKDS
                     ILKIYRSAVEVGAEWQPDLERQDVPAMVIWGRTDPYVPLAFGEALAERMKGELVVLEC
                     GHWWPFERPEETAQALLRHWSSAGS"
     gene            7831..>8013
                     /locus_tag="VFB52_01905"
     CDS             7831..>8013
                     /locus_tag="VFB52_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05114.1"
                     /translation="MPDRDLLSLHGLAVKKAGNAEAVAAILGERDAEAITAALDEHVA
                     AGRAAGANGMFMVAPPG"
BASE COUNT         1203 a         2872 c         2846 g         1092 t
ORIGIN      
        1 gtcgagatcg cctaccccgg catctccacc tggaaaatca ccgcgatcgg caagaccgcg
       61 cacccgaccg agcccgagca cgggatcaac gcggtgacca agatggcgaa gctggtcgag
      121 gcggtcgacc agaaccggct ggagctgccg aagggcgact cgcagtggtt cgagccgcgg
      181 gtcaccacca acggcatccg cacccgcccg ggcggcggct gggcgatccc ggggcgctgc
      241 gacgcggtgc tctcgattct ctcgcccccc ggcgcctcgc tgaacgaagt ccgcgacgcg
      301 gtcgacgcct tcctgcgcat gctcgagcag gaggacggcg aggtgaaata cgaacggaaa
      361 atcctgccga tgggcgccgg ccgtctctgg ctgcgcccgg gcgagtgcga cccggagcac
      421 gccggcgtca aggcgctcga ggcggcggtc cgcgagcacg gccagaagga ccccgaggtc
      481 cgcaagttca acggcggctg ggttgacgcc gtcgagctga tgcgccccgg cgccaacggc
      541 ttctcggaac cggcggtgat caccttcggg cccggtgact tcgaactcgc ccacaccgtg
      601 gacgagcaca tcccgatctc cgaagtcgcg gaagcggcgg agatctacgc agcggcgacc
      661 aagcagctac tcagctaacc agggttacta gggaaaaagg gagcagagag agatgaaaga
      721 gagtcccgca agcggcgccc tcaccgcctt ctcagcggtc gccgcggagc tcgaccgggt
      781 ggagaagatc gacgacctct tggtcgcgat cgcccgtcag atcagcgagc tggtcggtgt
      841 cgagcgctgc agcgtcttcc tccgcgacgg ccgtgaggac ttgttccgcg gctgcgtctc
      901 gcgcggggtc gacggaatcc acgacgcgga gatccgccag tgcgtggcgg ggatcccggc
      961 cgaccgggtg acccgcgagg cggtgcgcac gcgcaagccg gtgatcgtcg ccaacgcgcg
     1021 ctcggacaac cgcatggtca agtcgaccgt gcggcgctgg gagatccgct cggtgatgtg
     1081 ggtgccgatg gtgatcggcg aggagacgat cgggctgatc acgctcgacg acctcggccg
     1141 ccagcacgct ttcaccgagg ccgacgccga ggccgcgggc cgcttcgccg ggttggccgc
     1201 ggtcgcggtc gcccagaccc agcggcggct ggagctgagc tccaaactcg cggtcgccgg
     1261 ccgccaggtg cagtcgctgc gccgggcaac cgcggtcgac gagcagctga ccgacctggt
     1321 gctcgcgcgc aaaccgctcg aggacctgct cggcacgctc tcggagctgc tgatgaagcc
     1381 ctgcgcgatc ttcgacgacg ccggcaaccg ggtcgtcacc gcgctcccgg aaggggcgga
     1441 ggagagcgcc gtgccgcgcc tgctcgagcc ggagctggcg cactcgccgg aggtgcggcg
     1501 ggcgctggcc gaacacgacg gcagccgcgt cttcgtggtt gggccgctgc cgcaggtcaa
     1561 cctgctgcgg cgccacgtcg tcgccccggt gcagctcggc gaggaagtct ggggccggct
     1621 ggtcgtgatg gagcacaaga cgcggttcaa cggcggcgac atgatcgccg tgcgccgcgc
     1681 cgccgctctg atcgcgctcc aggtcagcgc cgaccgcaac gcggtcgagg ccgactggaa
     1741 cgccggctcc tcgctggccg ccgagctgct cggcggcagc tccgacctga ccaccgtgca
     1801 gcgccgcgcc gaccggctcg ggctgaacct ggaagctccg cgcgtggtga tgctgctggc
     1861 ggcgcgctcc ggcgacgagg aagaagcgcc ggacttccgc tcggtcgccg ccgccttcga
     1921 cgcggtcgcg ccgcaactgc aggtccaggt gaccgcggtc tccggcgccg ccgcggcgct
     1981 ggtcgagctg ccggtcgacg gtgcggcggg cgctgcggcg ctcgacttca aggacacgtt
     2041 cgaggcggtc cggatgcaca tcgacccgtc gctgatcggc ggcgtctccg gggtctactc
     2101 gacccgcgag ggctaccgca cggggcaccg cgaggcgcgt caggtgaccg agtgcgtgcg
     2161 ccgcttctcg ccgcccggtg ggccggcgct gttcaccgcc gaagagctcg gggtcggcag
     2221 cctgctgctc tccagctccg acagcgaggc ggtctcccag ttcgccgagc agacggtcgg
     2281 tgagctggtc cgcgagcact cgaaggccga cctgctgacc acgctctgct cgttcttcga
     2341 gaacatgggc tcgatccgcg gctcggcggc ggcgctcgac gtccacgaga acacgattcg
     2401 ctacaggctc tcccgggtcg aggagctgac cgggctggcg atcatgcacg atcccgacgc
     2461 ccagctccgc gcccgcctct cgctgctggt cctgatgctg aagggccgcc tgcccagcgg
     2521 cctctcggcc gccccggccc agagcgaggg catctccttg gaggtggtcg acgcggcagc
     2581 ctgagccgcg ccgccaagat cgcgcggttc ctttccggac acatggtccg gaaaggaacc
     2641 gcgcgagtct ttggagtaaa cgaagcgtcc ccgcaaatag cccgcgcgcg ggtcgatacg
     2701 ccctccctat cctcgggagg tgccgagggt tcacctgttg ggagtgggtg ccacgtctct
     2761 gtccagggag gctcgcgacg cgcgctggat gacccacgac gcggtcactg ccgccctcgc
     2821 cgacgccggg ctcgcgcccc gcgcggtcga cagcctggcg ctcgcctcgg gtgagcacgg
     2881 cgccacgctc ggctgccagg aggcgctcgg ccacccggtg cgctcgaccg aatgcgacag
     2941 cacccacggc gccctcgccc tgcacaccgc ctgggcggcg gtggcgacgg gggagagcga
     3001 cgtcgccgtc tgcgtcggcc acgacccgaa ccccgccggg gccgcgagcg tgctgccgat
     3061 cgacgcgatg gccgccgccg cccgtctcta catgcgcgaa tccggggcga ccgaggagca
     3121 cctggcccgg gtggcggcga agaaccgcgg tcacggcgcc gccaacccgc gggcgctggt
     3181 ccgcgagccg gtcgacgcca aggcggtgct cgccagcgcc ccgatggcct ggccgctgcg
     3241 ccggatgatg gtgccgctgc cggcccaggc cgccgccgcg gtggtgctcg gccgcgagga
     3301 ccggggccgc cgcgacggtc cgcgcgcgcc ccgcctgccc gccagcgtcc tgctcagcgc
     3361 cgacgagggc cgacccgcgg tcgcccgcgc cgccggcctc gcctacttcg gggcggcgct
     3421 gggcccggag gacgtcgacg tcgccgaggt cgaggacctc accccggcaa cagagatcgc
     3481 cgcctacgaa gccctggagc tcgcccccgc ccagcgcggc cccgagctgg tcgagacggg
     3541 gttcaccgaa ctgggcgggg tggttcccgt gaacgtcagc ggcgggtccc tggcccaggg
     3601 ccgcgtcccc ggcagcggcg cgatcctgca gttgatcgag ctcgcggccc agctccgcgg
     3661 ccgcgccggc cgccgccagg tgccgggcgc gcgcgtggga ttggcgttgt gcggcgactc
     3721 gatcggccca agggccgcaa cctcgctgac gatcgtcggc gcgtagcggc agccgggcca
     3781 gatcccacac ccacttcggc aggtaggtgt gggccctggc ccggctgacg ggagagggtt
     3841 gagttccggt gtgacttatg cccgcatagc gagcaaaact cacaccgcaa cgcgccagcg
     3901 ggccggcgcc cttgtaaccc ccacaacgcc tcgtaccccg ccgctaacgc ctcgcactcc
     3961 gcgatgcgag agtgaggtcc cgatgtcgcg cgctctctcg actgacgcca agaccctgcc
     4021 cgagctgctg cgcacgcgtg cggcggagat cggcgaggag cggttcgtcc gcgacgccca
     4081 ccaggagtgg acctacgctg agttcgccgc gcgggtcgcc gaggtcgcgg gcgggctgaa
     4141 ggctcgcggg gtgggggagg gcgacgtggt cggcgtggtg ttgccgaaca gcccgcacta
     4201 cctcgaggtc tggtggggca tcctgcagct gggcgcgacc ttcaacccgg tcaacccggc
     4261 gctgaccacg cgcgagcagg tcgggatcct cgccgactcg cgcgcctgct gcgtgatcgc
     4321 caccgaggac gccgctgccg gcctggagga aaaccgcgac cagctgccca acctgcgcga
     4381 gctggtcgtc gccggcgaga ccgacctggc ggcgccgctg cgcggcgaga cgccggtcga
     4441 ggagccggcc tccttcggcc ccgaccagat cgcctctttc gtctacacct ccggcaccac
     4501 cggccgcccg aagggcgcga tgctgagcca cggcaacctg ttgatgaacg cctggcagct
     4561 cggtgagccg ctaccggtgg gccgcggcga cacgatgggc atggtcctgc ccctgttcca
     4621 cgtcaacgcc caggtcgtca ccaccgtgat cccgctcttc ctcggcgccc agacggcgat
     4681 gtgggagcgc ttcagcgcct cgcgcttctg gcaggaggtc gccctcttcg agccggtcac
     4741 cttctcctcg gtgccgacga tgctggcggc gctgctgcac gcgcccggcg ccgacgaggc
     4801 cgagggcaac agcctgcgct tcgtgatctg cggcgccgcg ccgctctcgc ccgcgctctt
     4861 ccgccgcttc gaggagaagt tcgggctgaa gatcatggag ggctacgggc tcaccgaggg
     4921 cacctgctgc tcgaccatca accccttcta cgggccgcgc aaggtcggct cgatcgggct
     4981 gccgacccgc ggccaggagg tcgtgatctt cggcgatgac ggcgagatcg ccggccccgg
     5041 cacggtcggc gaggtcggga tccgcgggcg caacgtgatg gccggctact acgaacggcc
     5101 cgacgccaac gccgagaccc tgcgcgacgg ctggctgcac accggcgacg tcggctacca
     5161 ggacgaggac ggctacttct tcctcgtcga ccgggtcaag gacatgatca tccgcggcgg
     5221 cgagaacatc tacccgcgcg agatcgagga cgtcctcctc gaaaacgcca acgtcaaaga
     5281 ggccgcggtg gtcgggcgcc cggacgaggt ccgcggcgag gaggtgcacg cggtcgtcgc
     5341 gctggtcgag ggcgacgacg tggtcgtgct cgaggagcac tgccgggagc ggctcgctgc
     5401 cttcaaggtc ccctccagct gggaggtcgt cccggagctg ccgaagacct cgaccggaaa
     5461 gatcgacaag aagccgctgc gggcgaagct cgccgacgcg gcgtccaggt aaggagacgc
     5521 acggatgaga atgctcgacg gcaagtcgat tctgattacg ggagcggcca gcgggatcgg
     5581 cgaggcgacg gcgcgcaaag cggcctccga gggcgcccgg ctggtgctcg ccgacgtcaa
     5641 cgacgagaaa ggcgaggcct tccgggagga gctcggcggc gacgcgatct acgtcaactg
     5701 cgacgtgacc agcgaggctg cggtcgaagc cgcggtggcg aagacggtcg ccgagcacgg
     5761 ctcgatcgac ggcgccttca actgcgccgg gatcctgcac gcgctcggca acaccggcga
     5821 aaacccactc gacgaatggc agaaggtgat cgacgtcgat ctcgtcggcg tcttcctctg
     5881 caccaagcac gagatcctcg cgatggccgc ccagggctcg ggctcgatcc tcaacatggc
     5941 ctcggccgcc ggcctgatcg gctggcccgg cgcctccgcc tacgtcgccg ccaagcacgg
     6001 cgtcgtcggc ctgaccaaat cggcggccct ggagtacgcc gaggccggtg tccgggtcaa
     6061 ctcgatctgc ccttcctacg tgctcacccc gatggtcgag aaagacctct tcgaggacgc
     6121 gctcgggggc gacgaggagc tgatcgccgc ggcccgcgcc aaccacccga tcggccgctt
     6181 cgcccagccc gaggagatcg ccgaggcggc gacctggctg ctctccgacc gcgcctcctt
     6241 cgtcaccggc acggcgatga gcgtcgacgg cggttacacg gcgccctagc cgcgcaggta
     6301 gcgtcccgcc catggctgac agagacagtt tcgagcagag tccctacgag ttgccccccg
     6361 gctcgaccga ggtcgtgctg gtgcgccacg gcgcctccga ggcggccgtg gtcggcgccc
     6421 gcttcccgct cgtcgacggc cactcggacc cggccctcgc cgagtccggc aaggcccagg
     6481 ccgagatggt tgccgaggcg ctgcgcggcg aggaggtggc gggcatcttc gcctcgaccc
     6541 tgcgccgcac ccaggaatcg gcggcgcctc tggccgagga gctcgggctc gagccgcagg
     6601 tcctgttcga gctgcgcgag gtctacctcg gcgacttcga gggcggcgag taccggatcc
     6661 gccagggccg cggcgacccg atcatccagc aggtcttcgc cgaggaacgc tgggacgcga
     6721 tccccaacgc cgagtcgctc gacgacttcg gcgcccgact aaaaaagggg atcgagggga
     6781 tcgtcgccac cgtcgggccg gagacgaccg cgatcgcctt cgtccacggc gccgtgatcg
     6841 gccggctctg ccagatcgcg accgacagcc gccccttcgc cttcgtccac gccgacaact
     6901 gctcgatctc gcggatcgtc gtccacgccg acggcaagtg gctgctgcgg tccttcaacg
     6961 acatcaacca cctcaccgtc aagaccggcc cggccggctg agaggagctc gtaatgcccg
     7021 ccttcctcgt tcacggcaac cccgacagct cgcggctctg gaaccgggtg aagcagcacc
     7081 tcggctccta cgacggcgag gtcgtcgccg gcgacctgcc cggcttccgg gacccggcgc
     7141 cggagggatt cccgagcacc aaggagtcct acgtcgagtg gatctgcgag cagtgcgaag
     7201 cgctcggcgg caacgtcgac ctggtcggcc acgactgggg ctcgctgctg gtccagcggg
     7261 tcgcctcgat ccgccccgac ctgctcaaca ccgtcgcttg cggcggcgcc gtcgtcgacg
     7321 tgaactacga atggcacccg ctggcccagg tctggcagac gccggaagag ggcgagaggt
     7381 acatggccga ggagctgacc cccgagttcg ggatcgagca cctggtcgaa aacggggtgc
     7441 cgcgcgagga cgccgaggcc aatatctggc tgaccgagta cggcaaggac tcgatcctga
     7501 agatctaccg ctcggcggtc gaggtcggag ccgaatggca gcccgacctg gagcgccagg
     7561 acgtgccggc gatggtgatc tggggccgca ccgaccccta cgtgccgctc gcattcggcg
     7621 aagcgctggc cgagcggatg aagggcgagc tggtcgtgct cgagtgcggt cactggtggc
     7681 cgttcgagcg ccccgaggag accgcgcagg cgctcctgcg gcactggtct tcagcgggct
     7741 cctagcccgc tcccggcccg ggttgtgagc atcgacaacc cgcgttttac gcaggtggcg
     7801 aagccgttgc gtgcgtgaga ggctgggccg atgcctgatc gagatcttct ttccctgcac
     7861 ggactcgcgg tcaagaaggc cgggaacgcc gaggcggtcg ccgcgattct cggcgagaga
     7921 gacgccgagg cgatcaccgc cgccctcgac gagcacgtcg ccgccggccg cgctgccggc
     7981 gccaacggca tgttcatggt cgccccgccc ggg
//
LOCUS       DASRIG010000070         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2905836, whole genome shotgun sequence.
ACCESSION   DASRIG010000070 DASRIG010000000
VERSION     DASRIG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905836"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /gene="recN"
                     /locus_tag="VFB52_01910"
     CDS             complement(<1..1587)
                     /gene="recN"
                     /locus_tag="VFB52_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZO05115.1"
                     /translation="MLRELRIENLLLIEKAELRLGEGLNAITGETGAGKTVLAHSLDL
                     LMGGKAKSGVVRPGAEEAWVEGVFDLPPGLIDEPELAELAERLPEGAEEVVLGRRVSA
                     GGRTSAFVAGRAATAADLKLLGGRLVAFFGQHEHRRLTISSAQLEILDGFAGAEHLEL
                     RERYRESHRECVRLSAELAELREVEGSRERDLDLYRYELSEIDEVAPDPEERAGMADE
                     RERLRHAEGLREAAGGAHGALVGAEEDGGGAAAALALAEAQLNGAAGVDPKLDGVAER
                     VAALAVELADAGSELRDYAEGLEVDPGALLAIEERLEAIDRLERKHGGSVESVLAHAD
                     HCRAEIARLEGAEQRGAEAEAALAAAETRRAELGEQLSAGREAAAAPLEERVAAELNG
                     LSMEGARLEVRLEPHPDGFGPAGRETVELRVAPNPGIEAAPLRDAASGGELSRVMLAL
                     SGLGQAAAAGTFVFDEIDAGVGGNTARVVGERLRALGAEQQVLCITHLPQVASLADVH
                     FRLQKDTDTAQALARVEKLDG"
     gene            1667..3643
                     /locus_tag="VFB52_01915"
     CDS             1667..3643
                     /locus_tag="VFB52_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO05116.1"
                     /translation="METGTLARGDDALRLPRLRASAHGARRRWSAIGPGVRLILVLVL
                     ALVLTGGAAYLLISDEMRTDQIDAFSRLQEADARGLETVSAGLSEPAAVAAAGKGLEA
                     IREREGVSEALLIGPDGVVRASGGGKPVGSVDDDPSTLAILRGGPSYAGTEQDETEDK
                     SNFEFLVPIELGGERFVLETAYDSASFEDQLSHIREILALLMLAAIPTVLLAFYLLGG
                     RALLRGHRLALERATRDGLTDLPNHRAFQDEFEQAVSLAQRNGDPLALALIDLDNFKQ
                     VNDRHGHPEGDAILRRLAEILRNGRSADRPFRIGGDEFALMLQRTDAAGARRLSQRLA
                     KTLTSAGIPASVGVATLYPGENAKDLRSEADAALYEAKRAGGGRAALFADIRGPIAIT
                     TAAKRDAVNRLIDEGELETVFQPIWALDSELLVGLEALSRPDPDYGLTGPEEAFDIAE
                     QLGRVPELDRLCAATALGNADAVLPPQALLFLNIAPKSLELGSESVEWIAGQVEAAEL
                     ATEQVVIEVSERFPARTAPVADSLVALRKRGFQLALDDVGTGNSGLEMLRRIGADYVK
                     LDRSIVAAAATEPNARGVLMAMATYARQTGAFVIAEGIEDEETLAFLRGIQASDFAPQ
                     TIIQGGQGFALGAPDPQPSLEISRRSAARSPSGG"
     gene            complement(3603..5102)
                     /locus_tag="VFB52_01920"
     CDS             complement(3603..5102)
                     /locus_tag="VFB52_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05117.1"
                     /translation="MERKWWTLVLVSIATFMLLLDVTVVNVALPDIQRELNASLSSLQ
                     WVVDAYALTLAAFLLTAGSLGDRLGRRRVFTIGFGLFTFASFLCGIAEDPTLLNLARG
                     LQGIGAAGMFATSLALIGQEFHGKDRATAFGVWGAVVGGAVAVGPLVGGIVTEGLGWE
                     WIFFINVPIGIGAMVLTERKIVNVFAEKPEPVDLPGLVTFSAALFLLIFGLIRGNPEG
                     WGSSLILACLIGAAALLVLFVVIERRSRYPMLDLTLFRRPDFNGVSAVAFCLSAGMFA
                     MFLYMTIYMQGVLDYSPLEAGVRFLPLTILGFVVAPISGSLSHKVPIRILLGVGLTLV
                     GIGLLLMNGVSADSTWTTLLAGFIVAGIGIGTTNPGIGQAAIAVVPVEKSGMGSGINT
                     TFRQVGIATGVAGLGAVFQGRVDSKLHELVPNAPDGLGEVVASGGSRAVSELPLPPAL
                     HDKAVHAADIAFVSGFNTIILIAALISFVGAAAGFLLTRQKDFVQPTGG"
     gene            complement(5136..>5671)
                     /locus_tag="VFB52_01925"
     CDS             complement(5136..>5671)
                     /locus_tag="VFB52_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZO05118.1"
                     /translation="AAVGIKRALTGEVETVALPGLEMKTGEVSGLGVNDITLTRRPHD
                     RVAELSYRISGQEVGHVRCDGLVAATPAGSTGYNLANQGPILAWGVKGYVVSYIAPHS
                     LTARALVVAPGDVLHVGNASGREPVEVAVDGEPLTELDAGAELEVCFADAVASLAQLP
                     GSSFYGRIREKFGHLAV"
BASE COUNT          850 a         2071 c         2014 g          736 t
ORIGIN      
        1 gccgtcgagc ttctcgaccc gggccagggc ctgggcggtg tcggtgtcct tctgcagccg
       61 gaagtggaca tcggcgagcg aggcgacctg gggcaggtgg gtgatgcaga gcacctgctg
      121 ctcggcgccc agcgcccgca gcctctcccc cacgacccgc gccgtgttgc cgccgacccc
      181 ggcgtcgatc tcgtcgaaga cgaaggtgcc ggcggcagcg gcctggccga gcccgctgag
      241 cgccagcatc acccgcgaga gctcgccccc ggaggcggcg tcgcgcagcg gtgccgcctc
      301 gatcccgggg ttcggggcga cccgcagctc gaccgtctcg cgtccggcgg gaccgaagcc
      361 gtccgggtgc ggttccaacc gcacctcgag ccgcgcgccc tccatcgaga gtccgttcag
      421 ctcggcggcg acccgctcct ccagcggcgc cgcggcggcc tcgcggccgg cgctcagttg
      481 ctcgcccagc tcggcgcggc gggtctcggc cgcggccagt gccgcctccg cctccgcccc
      541 gcgctgctcg gcgccctcca ggcgcgcgat ctccgcccgg cagtggtcgg cgtgggcgag
      601 caccgactcg acgctgccgc cgtgcttgcg ttcgagccgg tcgatcgctt cgaggcgctc
      661 ctcgatcgcc aacagcgcac ccgggtcgac ctccaacccc tcggcgtagt cgcgcagctc
      721 cgagccggcg tcggccagct cgaccgccag cgccgcgacc cgctcggcga ccccgtcgag
      781 cttcggatcg acccccgccg caccgttcag ctgcgcttcg gccagggcca gcgccgcagc
      841 agcgccgccg ccgtcctctt cggccccgac cagggcgccg tgggcgccgc cggccgcttc
      901 gcgcaggccc tcggcgtggc gcagccgctc gcgctcgtcc gccatcccgg cccgctcctc
      961 ggggtcgggc gcgacctcgt cgatctcgga gagctcgtag cggtagaggt cgaggtcgcg
     1021 ctcccgcgag ccctcgacct cgcgcagctc ggccagctcg gcggacaacc gcacgcactc
     1081 gcggtgggac tcgcggtagc gctcgcgcaa ctccaggtgc tcggcgccgg cgaagccgtc
     1141 gaggatctcc agctgggccg aggagatcgt cagccgccgg tgctcgtgct ggccgaagaa
     1201 ggcgaccagg cgaccgccga gcagcttcaa gtcggcggcg gtcgcggcgc gcccggcgac
     1261 gaaggcgcta gtccggccgc cggccgagac ccgccgtccc aacaccacct cctcggcgcc
     1321 ctccggcagc cgctcggcca gctcggcgag ctccggctcg tcgatcaatc ccggaggaag
     1381 gtcgaagacc ccctcgaccc aggcctcctc ggcaccgggc cggaccacgc ccgatttcgc
     1441 cttgccgccc atcagcaggt cgagcgagtg cgccagcacc gtcttgccgg cgccggtctc
     1501 accggtgatc gcgttcagcc cctcgcccag ccgcagttcc gccttctcga tcagaagcag
     1561 gttctcgatt cgcagctcgc gcagcatcgg gtctatgtag caacggacgc ggaccgaacg
     1621 gaaatgccct gtaaaggggg atcacgaacg cgccgatatc actgttatgg agaccggaac
     1681 cctcgcccgg ggagacgatg ccctgcgcct gccgcggctg cgggccagcg cccacggcgc
     1741 ccgccgacgc tggtccgcga tcgggccggg cgtccgcctg atcctcgtcc tcgtgctcgc
     1801 cctggtgctg accggcggcg cggcctacct gctgatctcc gacgagatgc gcaccgacca
     1861 gatcgacgcc ttctcgcgcc tgcaggaagc cgacgcccgc ggcctcgaaa cggtgagtgc
     1921 ggggctcagc gagccggcgg cggtcgccgc ggcgggcaaa gggctggagg cgatccgcga
     1981 acgcgagggg gtcagcgagg cgctgctgat cggcccggac ggcgtcgtcc gggccagcgg
     2041 cggcggcaag ccggtcggca gcgtcgacga cgacccctcg acgctcgcga tcctgcgcgg
     2101 cgggccctcc tacgccggca ccgagcagga cgagacggag gacaagtcga acttcgagtt
     2161 cctcgtcccg atcgagctcg gcggcgagcg cttcgtgctg gagaccgcct acgacagcgc
     2221 cagcttcgaa gaccagctca gccacatccg cgagatcctc gcactgctga tgctggccgc
     2281 gatcccgacg gtcctcctcg ccttctacct gctcggcggc cgcgccctgc tccgcggcca
     2341 ccgcctggcc ctcgaacgcg ccacccgcga cgggctcacc gacctgccca accatcgtgc
     2401 cttccaggac gagttcgagc aggcggtctc gctggcccag cgcaacggtg acccgctcgc
     2461 cctcgccctg atcgacctcg acaacttcaa gcaggtcaac gaccgccacg gccatcccga
     2521 gggcgacgcg atcctgcgcc gcctggccga gatcctgcgc aacgggcgct cggccgaccg
     2581 gcccttccgg atcggtggcg acgagttcgc gctgatgctg cagcggaccg acgcggcggg
     2641 ggcgcggagg ctcagccagc gcctggcgaa gacgctgacc tcggccggga tcccggccag
     2701 cgtcggcgtc gccacgctct atcccggcga gaacgcgaag gacctgcgct ccgaagccga
     2761 cgctgccctc tacgaggcca agcgcgccgg gggcgggcgc gccgccctct tcgccgacat
     2821 ccgcggcccg atcgcgatca cgaccgcggc caagcgcgac gcggtcaacc ggctgatcga
     2881 cgagggcgag ctggagaccg tcttccagcc gatctgggcg ctcgactccg agctcctggt
     2941 cggcctcgag gcgctctccc gtcccgaccc cgactacggg ctcaccgggc ccgaagaagc
     3001 cttcgacatc gccgagcagc tcggccgggt gcccgagctg gaccggctct gcgcggcgac
     3061 ggcgctgggc aatgccgatg cggtccttcc tccccaggcg ctgctcttcc tcaacatcgc
     3121 gccgaagagc ctcgaactgg gcagcgagag cgtcgagtgg atcgccggcc aggtcgaggc
     3181 cgcggagctc gccaccgagc aggtggtgat cgaggtctcc gagcggttcc cggcgcgcac
     3241 cgcaccggtc gccgacagcc tcgtcgcgct gcgcaagcgc ggcttccagc tcgccctcga
     3301 cgacgtcggg accggcaact cggggctgga gatgctgcgg cggatcggcg ccgactacgt
     3361 caagctcgac cgcagcatcg tcgccgccgc ggcgaccgag ccgaacgcgc gcggggtgct
     3421 gatggcgatg gcgacctacg cgcgtcagac cggcgccttc gtgatcgccg aggggatcga
     3481 ggacgaggag acgctcgcct tcctgcgcgg catccaggcc agcgacttcg ccccgcagac
     3541 gatcatccaa ggcggccagg gcttcgcgct cggcgctccc gacccacagc cgtcgctgga
     3601 gatcagccgc cggtcggctg cacgaagtcc ttctggcggg tgagcaggaa gccggcggcc
     3661 gccccgacga aggagatcag cgcggcgatc aggatgatcg tgttgaagcc gctgacgaag
     3721 gcgatgtcgg cggcgtgcac ggctttgtcg tgcagcgcgg gcggcaacgg cagttcggag
     3781 acggcccgcg aaccgcctga ggcgaccact tcgccgaggc cgtccggcgc gttcgggacc
     3841 agctcgtgca gcttcgagtc aacccggccc tggaagacgg cgccgaggcc ggcgaccccg
     3901 gtcgcgatcc cgacctggcg gaaggtcgtg ttgatccccg agcccatccc cgacttctcg
     3961 accgggacga ccgcgatcgc cgcctggccg atgccggggt tggtcgtgcc gatcccgatc
     4021 ccggcgacga tgaagccggc gagcagggtc gtccaggtgg agtcggcgga gacgccgttc
     4081 atcagcagca gcccgatccc gaccagcgtg aggccgacgc caagcaggat ccggatcggc
     4141 actttgtggg agagcgagcc ggagatcggg gcgacgacga agccgaggat cgtcagcggc
     4201 aggaagcgca cgcccgcctc cagcggcgag tagtcgagca cgccctgcat gtagatcgtc
     4261 atgtagagga acatcgcgaa catcccggcc gagaggcaga aggcgacggc cgagacgccg
     4321 ttgaagtcgg ggcgccggaa cagggtcagg tcgagcatcg ggtagcggct gcggcgctcg
     4381 atcacgacga agaggacgag cagcgcggcg gccccgatca ggcaggcgag gatcaggctg
     4441 ctgccccagc cctcggggtt gccgcggatc aggccgaaga tcagcaggaa gagtgcggcg
     4501 gagaaggtga cgagcccggg caggtcgacc ggttcgggct tctccgcgaa gacgttgacg
     4561 atcttgcgct cggtcagcac catcgcgccg attccgatcg gcacgttgat gaagaagatc
     4621 cactcccacc cgaggccctc ggtgacgatc ccgccgacca gcgggccgac cgcgacggcg
     4681 ccgccgacca cggcgcccca gaccccgaag gcggtcgccc ggtctttgcc gtggaactcc
     4741 tggccgatca gcgccagcga ggtggcgaac atgccggcgg cgccgatccc ctgcaggccg
     4801 cgggcgaggt tgagcagcgt cgggtcctcg gcgatcccgc agaggaacga ggcgaaggtg
     4861 aagaggccga agccgatcgt gaagacgcga cggcgaccga gccggtcacc gagcgagccg
     4921 gcggtgagca ggaaggcggc caaggtcagc gcgtaggcgt cgaccaccca ctgcaggctg
     4981 gagaggctgg cgttcagttc gcgctggatg tccggcagcg cgacgttgac cacggtgacg
     5041 tcgagcagca gcatgaacgt cgcgatcgag acgaggacca gggtccacca cttgcgctcc
     5101 atggcgaatc accctaaaga agtgggttcg ccggcttaaa cggcgagatg gccgaacttt
     5161 tcgcggatgc ggccgtagaa actggagccc gggagttggg cgagggaggc gaccgcgtcg
     5221 gcgaagcaga cctccagctc ggcgccggcg tcgagctcgg tcagcggctc cccgtcgacg
     5281 gcgacttcga ccggctcgcg gccggaggcg ttgccgacgt ggaggacgtc gccgggggcg
     5341 acgaccaggg cgcgcgcggt cagcgagtgc ggggcgatgt aggagacgac gtaccccttc
     5401 acaccccagg cgaggatcgg gccttggttg gcgaggttgt agccggtcga accggcgggg
     5461 gtcgcggcga cgagcccgtc gcagcgcacg tgcccgacct cctggccgga gatccggtag
     5521 ctgagctcgg cgacgcggtc gtgcgggcgg cgggtcaagg tgatgtcgtt gacgccgagg
     5581 ccgctgacct cgcccgtctt catttcgagc ccggggagcg cgacggtctc gacttcgccg
     5641 gtcagcgcgc gcttgatccc gaccgcggct t
//
LOCUS       DASRIG010000071         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_809772, whole genome shotgun sequence.
ACCESSION   DASRIG010000071 DASRIG010000000
VERSION     DASRIG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809772"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="cysA"
                     /locus_tag="VFB52_01930"
     CDS             complement(<1..806)
                     /gene="cysA"
                     /locus_tag="VFB52_01930"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HZO05119.1"
                     /translation="MSIIVKDVSKSFGRFKAVDHVSLEIPTGSLVALLGPSGSGKTTL
                     LRIIAGLEIADDGAVLHGGEDITDYSARDRKVGFVFQHYALFRHMTIADNIGYGLRVR
                     GVKKADRAKRVEELLHLIRLDGFGDRYPSQLSGGQRQRVALARALAAQPNVLLLDEPF
                     GALDAKVRTELRQWLRRLHEEIHVTSVFVTHDQEEAFEVADHVVVMNEGKIEQVGTPA
                     EVFEHPANAFVMDFLGNVNVFHGRVEGGKAVVSGLEVEYPEYPHGEARDVA"
     gene            complement(803..1933)
                     /locus_tag="VFB52_01935"
     CDS             complement(803..1933)
                     /locus_tag="VFB52_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO05120.1"
                     /translation="MTVDTIAVTEKPRPRIGPSAIRRAQQDPAWVRWTLTGLALAVVG
                     VLIVLPVINVFAEALAGGVGVYWKNLFADPDTRHSILLTLTVVPFALVANVVFGVAAA
                     WAISRFQFRGRALLTALIDLPFSVSPVVAGLMFVLIFGLQGYFGGFLRRDGYSIMPYL
                     TSLVGVAVAIVVYFLVRPGDRRARARAVWKYPWLSILAGGAAVFAALFFLQQALDLWP
                     ANKSLKIIFATPGLILVTSFVTFPFVARELIPVMDAVGPEEELAALSLGASGWQMFWH
                     VTVPNVKWGLVYGIILCNARAMGEFGAVYVVSGHIAGQTDTMPLRIEKLFQEYNLPGS
                     FAVASVLTLVAIFTLIIKSQLERKVPSRLHGGKPDAAPGAHP"
     gene            complement(1923..2747)
                     /gene="cysT"
                     /locus_tag="VFB52_01940"
     CDS             complement(1923..2747)
                     /gene="cysT"
                     /locus_tag="VFB52_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZO05121.1"
                     /translation="MASVNRKILPGFSLSLGYTVFYLSVLVLVPIAACFIKAASLSFD
                     DFWGAVWTDRSRAAYLLTFGTSFAAALINLVLGALLAWVLVRYSFPGKRLIDALVDLP
                     LALPTAVGGLIYAGLYVENGWLGRFLVPLGIKAAYTRLGIVLVLVFVGLPFVVRTVQP
                     VLENLDAEAEEASASLGATRWQTFYTVILPALLPALITGFALAFARAVGEYGSVVFIS
                     GNMPYKTEIAPILIVARLEEFSYGEATAIAVVLLVISFSMLILINLLESWTRRHDR"
     gene            complement(2778..>3197)
                     /locus_tag="VFB52_01945"
     CDS             complement(2778..>3197)
                     /locus_tag="VFB52_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05122.1"
                     /translation="ARGATTTFVQKKIGDVHIAWENEAHLEVEEAKGELELVYPPISI
                     LAEPHVAIVDGNVDRKKTRAAAEAYLKYVYTDEAQEIVAKHFYRPSSEAVLKKHAADF
                     PELKRFPITDIAKDFPDAHKQFIGDGGVFDTIYKPKK"
BASE COUNT          548 a         1016 c         1176 g          457 t
ORIGIN      
        1 gccacgtctc gcgcctcgcc gtgcggatac tccgggtatt cgacctcgag gccggagacg
       61 accgccttgc cgccctcgac ccggccgtgg aacacgttga cgttgccgag gaagtccatc
      121 acgaacgcgt tggccgggtg ctcgaagacc tcggcgggcg tgccgacctg ctcgatcttg
      181 ccctcgttca tcacgacgac gtgatcggcg acctcgaacg cctcctcctg gtcgtgggtg
      241 acgaagacgc tggtgacgtg gatctcctcg tggagccggc gcagccactg gcgcagctcg
      301 gtccggacct tggcgtcgag ggcgccgaac ggctcgtcga ggaggagcac gttcggctgc
      361 gccgccagcg cgcgagccag ggcgactcgc tggcgctggc cgccggacag ctgcgacggg
      421 tagcgatcgc cgaagccgtc gaggcggatc aggtgcagga gctcctcgac ccgcttggcc
      481 cggtccgcct tcttgacgcc gcgcacgcgc aggccgtagc cgatgttgtc ggcgatcgtc
      541 atgtgccgga acagcgcgta gtgctggaag acgaagccga ccttgcggtc gcgggcggag
      601 tagtcggtga tgtcctcgcc gccgtgtagg accgcgccgt cgtcggcgat ctcgaggccg
      661 gcgatgatgc gcaggagcgt cgtcttgccg gagccggacg ggccgagcag ggcgaccagc
      721 gacccggtcg ggatctcgag gctgacgtgg tcgactgcct tgaagcggcc gaagctcttg
      781 gagacgtcct tgacgataat gctcatgggt gcgcccccgg ggctgcgtcg ggcttcccgc
      841 cgtggaggcg ggacggcacc ttgcgctcga gctgactctt gatgatcagg gtgaagatcg
      901 cgaccagggt caagaccgac gccaccgcga aggagccggg caggttgtac tcctggaaca
      961 gcttctcgat ccgcagcggc atggtgtcgg tctggccggc aatgtggccc gacacgacgt
     1021 agacggcgcc gaactcgccc atcgcgcgcg cgttgcagag gatgatgccg tacacgaggc
     1081 cccacttcac gttggggacg gtgacgtgcc agaacatctg ccacccgctg gcgccgaggc
     1141 tgagcgcggc gagctcctcc tcgggaccga ccgcgtccat gaccgggatc agctcgcggg
     1201 cgacaaacgg gaaggtgacg aaggaggtga ccaggatgag ccccggcgtc gcgaagatga
     1261 tcttgaggct cttgttcgcc ggccacaggt cgagcgcctg ctgcaggaag aacagcgcgg
     1321 cgaacacggc ggcgccgccg gcgaggatgc tcagccacgg gtacttccac acggcgcggg
     1381 cgcgggcgcg gcggtcgccg ggccggacca ggaagtagac gacgatcgcg accgcgacgc
     1441 cgacgagcga cgtcaggtac ggcatgatcg agtagccgtc gcggcgcagg aagccgccga
     1501 agtagccctg caggccgaag atgaggacga acatcaggcc ggcgaccacc ggcgacaccg
     1561 agaacggcag gtcgatgagc gcagtcagga gggcgcggcc gcggaactgg aagcgcgaga
     1621 tcgcccacgc ggcggcgacg ccgaagacga cgttggcgac cagcgcgaac gggacgaccg
     1681 tgagggtcag caggatcgag tgccgggtgt cgggatcggc gaacaggttc ttccagtaga
     1741 cgccgacgcc gccggccagc gcctcggcga agacgttgat caccggcagc acgatgagca
     1801 cgccgacgac cgcgagggcg agcccggtca aggtccagcg gacccaggcc ggatcctgct
     1861 gggcgcggcg gatcgccgac ggcccgatcc ggggccgcgg cttctcggtg accgcgatgg
     1921 tgtcaacggt catggcggcg cgtccagctc tcgaggaggt tgatcaggat cagcatcgag
     1981 aaggagatga ccagcagcac gaccgcgatc gcggtcgcct cgccgtacga gaactcctcg
     2041 aggcgggcga cgatcaggat cggggcgatc tcggtcttgt acggcatgtt gccggagatg
     2101 aagacgaccg agccgtactc gccgaccgcg cgggcgaagg cgagggcgaa gccggtgatc
     2161 agggccggga ggagcgccgg caggatcacg gtgtagaagg tctgccagcg ggtcgcgccc
     2221 agcgacgccg acgcctcctc ggcctcggcg tcgaggttct cgagcaccgg ctgcacggtg
     2281 cggacgacga acggcaggcc gacgaagacc agcaccagca cgatgccgag ccgggtgtag
     2341 gccgccttga tgccgagcgg gaccaggaag cggccgagcc agccgttctc gacgtagagg
     2401 ccggcgtaga tgaggccgcc gaccgcggtc ggcagcgcga gcggcagatc gaccagcgcg
     2461 tcgatcagcc gcttgccggg gaacgagtag cggaccagca cccacgccag cagcgcgccc
     2521 aggaccaggt tgatcagcgc agccgcgaac gaggtgccga aggtgagcag gtacgcggcg
     2581 cgcgaccgat cggtccacac cgcgccccag aagtcgtcga acgacagcga cgccgccttg
     2641 atgaaacagg cggcgatcgg cacgagcacc aggacgctca ggtagaagac cgtgtagccg
     2701 aggctgaggg agaaccccgg caggatcttt cgattgacgc ttgccatgtc gccgccgcgc
     2761 tcgcgccgag ctacgtccta cttcttcggc ttgtagatgg tgtcgaacac cccgccgtcg
     2821 ccgatgaact gcttgtgggc gtcggggaag tccttggcga tgtcggtgat cgggaagcgc
     2881 ttgagctcgg gaaagtcggc ggcgtgcttc ttcaggaccg cctcgctgct ggggcggtag
     2941 aagtgcttgg cgacgatctc ctgcgcctcg tcggtgtaga cgtacttgag gtaggcctcg
     3001 gcggcggcgc gggtcttctt gcgatcgacg ttgccgtcga cgatggcgac gtgcggctcg
     3061 gccaggatgc tgatcggcgg gtagaccagc tcgagctcgc cctttgcctc ttcgacctcg
     3121 aggtgggcct cgttctccca ggcgatgtgg acgtccccga tcttcttctg gacgaacgtg
     3181 gtggtcgcgc cgcgggc
//
LOCUS       DASRIG010000072         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_557970, whole genome shotgun sequence.
ACCESSION   DASRIG010000072 DASRIG010000000
VERSION     DASRIG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557970"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="lexA"
                     /locus_tag="VFB52_01950"
     CDS             complement(<1..549)
                     /gene="lexA"
                     /locus_tag="VFB52_01950"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZO05123.1"
                     /translation="MVDLDLTKRQKEIFDFIGKYAAKTGYPPTVREIGKAVGLHSSST
                     VHAHLANLEKIGLLKRDPSKPRAIELLIGTANKAKDAIRPPSGLPLVGQVAAGAPILA
                     EENIEEYLEVPDVIGGEDGDYILQIRGESMKNAGILEGDYVVVRPSDDADNGEIVVAL
                     IGEEATVKRIYCEADHIRLQPEN"
     gene            686..771
                     /locus_tag="VFB52_01955"
     tRNA            686..771
                     /locus_tag="VFB52_01955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:720..722,aa:Leu,seq:gag)
     gene            908..1570
                     /locus_tag="VFB52_01960"
     CDS             908..1570
                     /locus_tag="VFB52_01960"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZO05124.1"
                     /translation="MPQKRRDPYGEALTLSQLFDDQKREFLAAATRPQGRSSTRRRAR
                     QSIPELEHGGAALRLGTWSAPECSVRVMKFRAAIRLAGKTATGFEVPPEVVDGLGAGK
                     KPPVRVTINGKTYRSTIASRGERYMVGVSAENRQLTGVAAGDEVEIELTLDTEPREVT
                     VPPDLADALTADTEAKRFFEGLTFSQKQWYVLPISQARKTDTRARRVVKAIAMLREGR
                     KR"
     gene            complement(1685..2569)
                     /locus_tag="VFB52_01965"
     CDS             complement(1685..2569)
                     /locus_tag="VFB52_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZO05125.1"
                     /translation="MIAPSTAPRGTLLQDTIDYWEGLGHPVVVAENVGQTLGGYLGGS
                     PAQRAADLNAMIASAEVELIVSAMGGKGAVHLLPLIDYEAFAARPKLFMGLSDVSLLV
                     LALHARTSVVTFHGPTGMDFGALPEYTARAMLGALRATEPLGELAPHGEWRALRGDER
                     ASGPLLGGHLGTIRSLLGTPYAPDFAGAVLFLEEIDAELHDVDVSLSHLALAGVFDHI
                     AALVVGRPVAVEERWRDSDEQMSDVVLRNCGHYGFPILYDVDLGHTEEKVTLPVGTVA
                     TVDPRRGTLSLDQPAVQG"
     gene            complement(2614..3357)
                     /locus_tag="VFB52_01970"
     CDS             complement(2614..3357)
                     /locus_tag="VFB52_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO05126.1"
                     /translation="MTALRLQGLRKEFGGIVALEGIDLEVERGEIVAVVGDNGAGKST
                     LIKLISGVHRPTSGMLYVDGKPQRFHDPIAARAAGIEVVYQDLALVDHQPVYMNLFLG
                     RELITRFRRLDRKRMSRETQQLVDELDIRIPSAQVRISTLSGGQRQGIAISRATHWAR
                     SLVLLDEPTAALGVAETAKVEETIAQLRGRGLAMVVVSHNLDQVFRLADRVVVLRRGV
                     HVATRRIAEIDRNEIVAMITGLSTGGAFA"
     gene            complement(3354..4379)
                     /locus_tag="VFB52_01975"
     CDS             complement(3354..4379)
                     /locus_tag="VFB52_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05127.1"
                     /translation="MSAEPSISPLASPGQQTGAGGGALRRLGRRLRGVDAGRYAVYVG
                     FLAILILFSITLSDEGFTSSRNLLNILEATAPITVMAVATVFVLGAGEIDLSIGAVVA
                     LASLVAALVMRETGAAVLGVAAGLGVGAGVGLLNGILVTRLRLPSFLVTLGTMGLVTG
                     LAQRITNLESIPSVIEPFNDVFGSGELLGVSTLIVWTAVAVAIGHYVLRHSRTGAHVL
                     ASGDNADAAQVSGVRVGRVKTGVLVASGIAAAFAGLMYTANLHGATYTLGSSDLLTVI
                     AAVVIGGTRLFGGRGTVIGALVGSLLLGIVDNGLILSGFSSSEQMIAQGAIILIAVAL
                     TLREPAR"
BASE COUNT          759 a         1493 c         1523 g          674 t
ORIGIN      
        1 gttctcgggc tgcaggcgga tgtggtcggc ctcgcagtag atccgcttca cggtcgcctc
       61 ctcgccgatc agggcgacga cgatctcgcc gttgtcggca tcgtcggagg gacggacgac
      121 gacgtagtcg ccctcgagga tgccggcgtt tttcatgctt tcgccgcgga tctggaggat
      181 gtagtcgccg tcctcgccgc cgatcacgtc gggcacttcg aggtattcct cgatgttctc
      241 ctcggccagg atcggcgcac cggcggcaac ctgaccaacg agcggcaacc cactcggcgg
      301 ccggatcgcg tccttggcct tgttcgccgt cccgatcaaa agctcaatag cccgcggctt
      361 cgagggatcc cgcttcagca gcccgatctt ctccagattg gccaggtgcg cgtgcacggt
      421 cgaggacgag tgcagcccca ccgctttgcc gatctcccgc acagtcggcg ggtacccggt
      481 cttggccgcg tacttgccga tgaagtcgaa gatctccttc tgccttttgg tcagatcaag
      541 gtcaaccatc actcggtctc gtttccctcc gcttgccggg tcctttccaa ctgccgaaca
      601 cttgttcgta gtctaccccc tccccccgga cgcccgcaag ccaacggcgc gcccagagcc
      661 caaaccccta gaatccgccc accccgcggc tgtggcggaa ctggtagacg cgcaaggttg
      721 agggccttgt gggtggaaac acccgtgatg gttcgactcc attcagccgc atggaaggaa
      781 tccccgctct ggcggggatt ctgcgttgtg gggacagcag cgcgcgcgcc ggcagccacc
      841 cggacctgga agacgccttg gccggactcc gcatccgatt cagcgaagcg gaaccgattg
      901 ggcggggatg ccgcagaaac ggcgcgatcc ctacggcgag gccctgacgc tgagtcagct
      961 tttcgacgat cagaaaaggg aattcctcgc ggcagccaca aggccgcagg ggcgaagttc
     1021 aaccaggcgg cgcgcgaggc agtcgatccc ggagcttgaa cacggaggtg ccgccttacg
     1081 tcttgggacc tggtcggcgc ctgaatgtag tgtccgggtg atgaagttcc gtgctgcgat
     1141 ccgcctcgcc gggaaaaccg cgaccgggtt cgaagttccc cccgaggtcg tcgatggctt
     1201 gggtgccggc aagaagccac cggtccgcgt gaccatcaac ggcaagacct accgcagcac
     1261 gatcgcctcc cgcggtgagc gctatatggt cggcgtcagt gcagagaacc gccagctgac
     1321 cggtgttgcc gccggcgacg aggtcgagat cgagctcacc ctcgacaccg agccgcgcga
     1381 ggtcacggtg ccccccgact tggccgacgc cctcacggcg gacaccgagg ccaagcgctt
     1441 cttcgagggg ctcaccttca gccagaagca gtggtatgtg ttgccgatct cccaggcgag
     1501 gaaaactgac actcgggctc ggcgggtcgt caaggcgatc gccatgctgc gggaaggccg
     1561 taagcgctga acaacagttg gtcgtcccac ggattgggca cgacgcatgc gctggcatcc
     1621 aggactcgaa ccgccgacgc ggccgagtgg gttggcttgg gactcgccgg cccgagctag
     1681 ccgcctagcc ctggacggcc ggctggtcga gcgagagcgt cccgcggcgg ggatcgaccg
     1741 ttgccacggt tccaaccggc agggtcacct tctcctcggt gtgccccaga tccacgtcgt
     1801 agaggatcgg gaagccgtag tggccgcagt tgcgcagcac gacgtcggac atctgctcgt
     1861 cggaatcgcg ccagcgctcc tcgaccgcga ccggacgacc cacgacgagg gcggcgatat
     1921 ggtcgaacac gccggccagg gcaaggtggg aaagcgagac gtcgacgtcg tgcagctccg
     1981 catcgatctc ctcgaggaag agcacggcgc ccgcgaagtc gggcgcgtac ggggttccga
     2041 gcagcgatcg gatcgtcccc agatggccac ccagcaaggg accgctggcg cgctcgtctc
     2101 cgcgcagcgc ccgccactcg ccatgcggcg ccagctcgcc cagcggctca gtggcccgca
     2161 gcgcgcccag catcgcccga gccgtgtact cgggcagagc cccgaagtcc atcccggtcg
     2221 ggccgtggaa ggtgacgacg ctggttcggg catgcagcgc gagcaccaag agcgagacgt
     2281 cggagaggcc catgaacaac ttcggcctgg cggcgaaggc ctcgtagtcg attagcggca
     2341 agaggtgcac tgcgcccttt ccccccatcg ccgagacgat cagctcgacc tccgcggagg
     2401 cgatcatggc gttgaggtcg gccgcgcgct gcgcgggtga gccaccgaga tacccgccga
     2461 gggtctgccc gacgttctcg gctacgacga cgggatggcc gagcccctcc caataatcga
     2521 tcgtgtcctg cagcagggtt ccgcgcgggg cagtcgacgg cgcgatcacg ccaaccgtcg
     2581 cgccgggacg aagcgccggg ggaaggatcg gattcaggcg aaagcccccc cggtgctcag
     2641 ccccgtgatc atcgccacga tctcgttgcg atcgatctcg gcgatgcgcc gggtggcgac
     2701 gtgaacgccg cggcggagta cgacgacgcg atcggcgagg cggaagacct ggtcgaggtt
     2761 gtgactgacg acgaccatcg ccaggccgcg ccctcgcaac tgggcaatcg tctcctcgac
     2821 ctttgccgtc tcggccacgc cgagagcggc ggtcggctcg tcgagcaaga ccagcgaccg
     2881 ggcccaatgg gtggcgcggc tgatcgcgat gccctgccgc tgacccccgg acagggtgct
     2941 gatccgaacc tgcgcggagg ggatcctgat gtcgagctcg tcgacgagct gctgggtttc
     3001 gcgggacatc cgcttgcggt cgaggcggcg aaacctggtg atcagctcgc ggccgaggaa
     3061 gaggttcatg tagaccggct ggtggtcgac cagcgcgagg tcctggtaaa cgacctcgat
     3121 gccggccgcg cgcgcggcga tcgggtcgtg gaagcgctgt ggtttcccat cgacgtagag
     3181 catgccactg gtcggcctgt ggacccccga gatcagcttg atcagggtcg acttgccggc
     3241 accgttgtcg ccgacgacgg cgacgatctc gccgcgctcg acctcgaggt cgatgccctc
     3301 gagcgcgacg atgccgccga actccttgcg caggccctgc agccgcagcg ccgtcatcgg
     3361 gcgggctcgc ggagagtcaa ggcgacggcg atcaggatga tcgcgccctg ggcgatcatc
     3421 tgctcggagc tggagaagcc cgagaggatc aggccgttgt cgacaatgcc gagcagcagc
     3481 gagccgacca gggcgccgat cacggtcccg cgcccgccga acagccgcgt gccgccgatc
     3541 acgaccgcgg cgatcacggt cagcaggtcg ctcgagccca gcgtgtaggt ggcgccatgc
     3601 aggttcgccg tgtacatcag cccggcgaac gccgccgcga tgccgctggc gacgagcacc
     3661 ccggtcttga cccggccgac ccggacgccg gatacctgcg cggcgtccgc gttgtcgccg
     3721 ctcgcgagca cgtgggcccc ggtccggctg tggcggagga cgtagtggcc gatcgcgacg
     3781 gcgacggccg tccagacgat cagggtcgaa acgccgagca actcgcccga accgaagacg
     3841 tcgttgaagg gctcgatcac cgaaggaatc gattcgaggt tggtgattcg ctgcgcgagc
     3901 ccggtgacca ggcccatcgt cccgagcgtg accaggaacg acggcagccg cagacgggtg
     3961 accaggatgc cgttgagcag gccgacgccg gcaccgactc cgagcccggc ggctacgccg
     4021 agcaccgccg cgccggtttc gcgcatgaca agcgcggcga cgagactggc gagcgcgacg
     4081 accgccccga tcgagaggtc gatctcaccc gccccgagca cgaagacggt ggcgaccgcc
     4141 atgaccgtga tcggtgcggt cgcttcgagg atgttgagca ggttgcggct gctggtgaag
     4201 ccctcgtcgc tgagcgtgat gctgaacagg atcaggatcg ccaggaatcc gacgtaaacg
     4261 gcatagcggc cggcgtcgac cccgcgcaac cgccggccaa gccggcgaag ggcgccgccg
     4321 ccagccccgg tctgttgacc ggggctggcg agcggcgaga tggagggctc agcggacata
     4381 cctctgggac cgctacttca gggcgtcgag agccgccgcg gggggactct ggtgcagcga
     4441 ttcctcgta
//
LOCUS       DASRIG010000073         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_293159, whole genome shotgun sequence.
ACCESSION   DASRIG010000073 DASRIG010000000
VERSION     DASRIG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293159"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VFB52_01980"
     CDS             <1..1417
                     /locus_tag="VFB52_01980"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZO05128.1"
                     /translation="LASFAVESPLTGFGAGGYMLVHRETGAGAEQTLIDFFVAAPGLD
                     GVERRAELVPVPVHFDAETSQTFFVGPASCGVPGVPAGLVRALERFGTMPLAALAEPA
                     VRLARVGAPVNAQQAYLLEILEPIHARLPGTRELYAPNERRLREGETFRFTELAESLE
                     RFAAEGAEAFYRGEVGTALADFVIAAGGTLGHADLAAYEAIERRPISADFRGTEVFTN
                     PPPSSGGILLAYSLGLLERLGPASGPEQLVAALAAANDRRSLEFAEALYEEGMEASFL
                     DAAGLDLAAADLLGSTTHVSVLDDAGTCASVTCSNGSGSGVLVPGTGVILNNMMGEED
                     LHPLGFHRIAPGRRVSSMMAPTVVLRDGEIVLGVGSAGSNRIRSAILQTVVGVVEQQL
                     TAEAAVRAPRLHFEAGAVQAEPGLDEAALARIEARGTPVFRWPAINLFFGGVQAVARE
                     PGSGALSGGGDPRRGGAVASA"
     gene            1414..2523
                     /locus_tag="VFB52_01985"
     CDS             1414..2523
                     /locus_tag="VFB52_01985"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HZO05129.1"
                     /translation="MIDFEAEGLLDGVEGDARKARRELLEQLTAEGVALEELREAAAA
                     GRLALIPVEHAIAGDGVRYTAREVAEKTGIDLELLRRFRASLGVPYADPDERIATEAD
                     LQAAQRTKRILDAGFPPEDLLRNARTIGMGMGRIAEANRELVVRNLTNPGETERDVAN
                     NLKLVAEALLPLVGDTLTYAFQVNLLEQVKRDVIAASDLASGELGGTVELSVCFADLV
                     EFTRLGEEIEPEELGQVAGRLEEMATAVAEPPVRLVKTIGDAAMLVSTEPAALTRAAL
                     ALIAAAEDEGERFPLLRAGLVTGTALPQAGDYYGRAVNLASRVTGVARPGSCVVDEAT
                     KEAIGEEFFRFTYIGERRLKGIEGRTKLFRVRLRF"
     gene            complement(2524..3444)
                     /locus_tag="VFB52_01990"
     CDS             complement(2524..3444)
                     /locus_tag="VFB52_01990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO05130.1"
                     /translation="MPASPSATPEHIKDVNTRYHDAAAEEYDAKWGIDFGDIGQEQVR
                     LKLVKALGADHGRSFGDALEIGSGTGYFSLNLVQLGVIERLTATDISPGMLKKLAATA
                     EALGVENVSTVATEAEELPFEDESFDLVFGHAVLHHIPDLDKAFAEFRRVLRPGGMVA
                     FAGDPSRYGDRLAALPKRTGMFVAPAWRRLVGARHRAVAESEQSHDHSLESEVDVHAF
                     APADLRRILATSGFEDRRVGGEELLSNAWGWGLRTVEASAEPDSVPFSWRRFAFRSYI
                     ALQKVDTRFLEPHLPAELFYNLLVSGRKPI"
     gene            3514..4998
                     /locus_tag="VFB52_01995"
     CDS             3514..4998
                     /locus_tag="VFB52_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZO05131.1"
                     /translation="MVLLSVLCALAAALVATSAQAADRVYWAAYNTGEVYWSNLDGSG
                     GIHELNSTGATISPMGYGFGGAIDPVQGRYYWANQIDGVINWAALDGSGGGVLPTGAA
                     TVSGPLGVSVDPVARRAYWSNETGSISYANLDGSGGQNLSTTGASVSSPTGTTVFPAS
                     GRVYWTNWGFLSYWISYAATDGSGGQTLNVTGATMDTPFGIAIDAAKQRLYWANDNPG
                     IISVANLDGTGASDIANSGLKIYGPYGMALDPEAGIAYTANFAGNDLTALRVDGSGGR
                     SLPIVLPKESGPNFPVLYKDPKAVSAPTVKRQPAPLPKKKLKGRPLPKLLGSRLTCAA
                     ATWQPDLVEARLYQAPTSVALAWTKDGADLGPVAGALRANRVGNYRCRATGTNVAGST
                     AATSGAVAIFATGKVQANKKKGTARLTVRLPAEPGRLVLAGKGFKAVAKRASGKTTVL
                     IKPKGAKKAKLAEQGSLRAVVKLTYTPPDGLAATLRTRVALVQG"
BASE COUNT          750 a         1794 c         1848 g          700 t
ORIGIN      
        1 gctcgcctcg ttcgcggtcg agagcccgct gaccgggttc ggcgccggcg gctacatgct
       61 cgtccaccgc gagaccggtg ccggcgccga acagacgctg atcgacttct tcgtcgccgc
      121 ccccggtctc gacggggtcg agcgacgcgc cgagctggtc ccggttccgg tccacttcga
      181 cgccgagacc tcgcagacgt tcttcgtcgg cccggcctcc tgcggcgtgc ccggcgttcc
      241 cgccgggctg gtccgggcgc tggagcggtt cgggacgatg ccgctggcgg cgctggccga
      301 gccggcggtg cgcctggccc gggtcggggc gccggtcaac gcccagcagg cctacctgct
      361 ggagatcctc gagccgatcc acgcgcgcct gccgggcacg cgcgagctct acgcgccgaa
      421 cgagcgccgc ctgcgcgagg gcgagacctt ccgcttcacc gagctggcgg aatcgctcga
      481 acggttcgcc gccgaggggg cggaggcgtt ctaccgcggt gaggtcggca ccgcgctcgc
      541 cgacttcgtg atcgccgcgg gcggcacgct cggtcacgcc gacctggccg cctacgaggc
      601 gatcgagcgg cgcccgatca gcgccgattt ccgcggcacc gaggtcttca ccaacccgcc
      661 gccgtcctcg ggcggcatcc tgctcgccta cagcctcggg ctgctggagc ggctgggccc
      721 cgcgagcggc cccgagcagc tggtcgcggc gcttgccgcc gccaacgacc ggcgcagcct
      781 cgagttcgcc gaggcgctct acgaggaagg catggaggcg agcttcctcg acgccgccgg
      841 gctcgacctc gccgccgccg acctgctcgg ctcgaccacc cacgtctcgg ttctcgacga
      901 cgccggcacc tgcgccagcg tcacctgctc caacggctcc ggctcggggg tgttggtgcc
      961 ggggacgggg gtaatcctca acaacatgat gggcgaggag gacctgcacc cgctcggctt
     1021 ccaccggatc gcgcccggcc gccgcgtctc ctcgatgatg gcgccgaccg tggtcctgcg
     1081 cgacggcgag atcgtgctcg gcgtcggcag cgccggctcc aaccggatcc gctcggcgat
     1141 cctgcagacc gtggtcggag tggtcgagca gcagctgacc gcggaggcgg cggtgcgggc
     1201 gccgcggctg cacttcgagg cgggcgcggt gcaggcggag cccggtctcg acgaggccgc
     1261 cttggcgcgg atcgaggccc ggggcacgcc ggtcttccgc tggcccgcga tcaacctatt
     1321 cttcggcggc gtgcaggcgg tggcgcgcga accgggcagc ggagcactca gcggcggcgg
     1381 cgatccgcgt cgcggcggag cggtggcgag cgcgtgatcg acttcgaggc ggaggggctg
     1441 ctcgacggcg tcgagggcga cgcccgcaag gcgcggcggg agctgctcga gcagctgacc
     1501 gccgaaggcg tcgcgctgga ggagctgcgc gaggccgcgg ccgcgggccg gctggcgctg
     1561 atcccggtcg agcacgcgat cgccggcgac ggcgtccgct acacggctcg cgaagtcgcc
     1621 gagaagaccg gcatcgacct cgagctgctg cgtcgcttcc gggcgtcgct cggggttccc
     1681 tacgcggatc ccgacgaacg gatcgcgacc gaggcggacc tgcaggcggc gcagcggacc
     1741 aagcggatcc tcgacgccgg gttcccgccc gaggacctgc tgcgcaacgc gcgcacgatc
     1801 gggatgggga tgggacggat cgccgaagcc aaccgcgagc tggtcgtccg caacctgacc
     1861 aaccccggcg aaaccgagcg cgacgtcgcc aacaacctga agctggtggc cgaggcgctg
     1921 ctgccgctgg tcggcgacac cctcacctac gccttccagg tcaacctcct cgagcaggtc
     1981 aaacgcgacg tgatcgcggc ctccgacctc gcctcggggg agctgggggg gacggtcgag
     2041 ctgagcgtct gcttcgccga cctggtcgag ttcacgcggc tcggcgagga gatcgagccg
     2101 gaggagctgg gccaggtcgc gggccggctg gaggagatgg cgaccgcggt cgccgagccg
     2161 ccggtgcgcc tggtgaagac gatcggcgac gccgcgatgc tggtctccac cgagcccgcc
     2221 gcgctgaccc gggcggcgct ggcgctgatc gccgccgccg aagacgaagg cgagcgcttc
     2281 cccctgctgc gcgccgggct ggtcaccggc acggcgctgc cgcaggcggg cgactactac
     2341 ggacgcgccg tcaacctcgc cagccgggtt accggcgtcg cccgtccggg cagctgcgtg
     2401 gtcgacgagg cgaccaagga agcgatcggc gaggagttct tccgcttcac ctacatcggt
     2461 gagcggcggc tgaaggggat cgaaggccgg acgaagctgt tccgggtccg gctgcggttc
     2521 tagtcagatc ggcttgcggc cggagacgag caggttgtag aagagctcgg cggggaggtg
     2581 gggctcgagg aaccgggtgt cgaccttctg cagggcgatg tagctgcgga aggcgaagcg
     2641 gcgccagctg aaggggaccg agtcgggctc ggcgctcgcc tcgaccgtgc gcaggcccca
     2701 gccccaggcg ttggagagca gctcctcgcc gccgacccgg cggtcctcga agccagaggt
     2761 cgccagtatg cggcgcaggt cggcgggggc gaaggcgtgc acgtcgactt cgctctccag
     2821 cgagtggtca tgggactgct cggattcggc gacagcgcgg tggcgggcgc cgaccagccg
     2881 ccgccaggcg ggggcgacga acatgccagt gcgcttcggc agcgccgcca gacggtcgcc
     2941 gtagcgcgag gggtcgccgg cgaaggcgac catgccaccg ggccgcagga cccggcggaa
     3001 ttcggcgaag gccttgtcga ggtcggggat gtggtggagg acggcgtggc cgaaaacgag
     3061 gtcgaaactc tcgtcctcga acggcagctc ctcggcctcc gtggcgaccg tgctgacgtt
     3121 ctcgacgccg agcgcctcgg cggtggccgc cagcttcttc agcatccccg gcgagatgtc
     3181 ggtcgccgtc agccgctcga tcaccccgag ctggaccagg ttgagcgaga agtagccggt
     3241 gccggaaccg atctccaggg cgtcgccgaa gctgcggccg tgatcggccc ccagtgcctt
     3301 gaccagcttc aggcgcacct gctcctggcc gatgtcaccg aagtcgatgc cccacttggc
     3361 gtcgtactcc tcggcggcag cgtcgtggta gcgggtgttg acgtctttta tgtgctccgg
     3421 ggtggcggag ggagaggccg gcatgcgcgg ggcactctaa aacaccttgc gcgtagtacg
     3481 gtctttgagg tgatcgtccg cctcggcaga agggtggttc tgctttcggt cctctgcgcg
     3541 ctcgcggccg ctctcgtcgc gacctccgca caggccgcgg acagggtcta ctgggctgcc
     3601 tacaacaccg gcgaggtcta ctggtcgaat ctcgacggca gcggcggtat ccacgagctg
     3661 aactccaccg gcgcgacgat ctcgccgatg ggctatggct tcggcggtgc gatcgacccg
     3721 gttcagggcc gctactactg ggcgaatcag atcgacggag tgatcaactg ggccgcgctc
     3781 gacggcagcg gcggcggtgt tctgccgacc ggcgcggcga ccgtgagcgg gccgctcggc
     3841 gtctccgtcg acccggtcgc gcgccgcgcc tactggtcga acgaaaccgg ttccatctcc
     3901 tacgcgaacc tcgacggcag cggtgggcag aacctctcaa ccaccggggc ctccgtttcc
     3961 tcaccgaccg ggaccaccgt gttcccggcc tcgggacgcg tctactggac caactggggc
     4021 ttcctctcct actggatctc ctacgcggcc accgacggca gcggcgggca gacgctgaac
     4081 gtcaccggcg ccaccatgga caccccgttc gggatcgcga tcgacgccgc aaagcagcgc
     4141 ctctactggg cgaacgacaa ccccgggatc atctcggtcg ccaatctcga cggcaccggc
     4201 gcctccgaca tcgccaacag cggcctgaaa atttacggcc cctacgggat ggcgctggac
     4261 ccggaagcgg ggatcgccta caccgccaac ttcgccggca acgacctgac cgcgctgcgg
     4321 gtggacggca gcggcggcag gtcgctgccg atcgtcctgc cgaaagaaag cggccccaac
     4381 ttcccggtgc tctacaagga cccgaaggcg gtgtcggcgc cgacggtcaa acggcagccc
     4441 gcaccgctcc cgaagaaaaa attgaaaggg cggccgctgc cgaagctgct cggctcgcgg
     4501 ctcacctgcg cggccgcgac ctggcagccg gacctggtcg aggcccggct ctaccaggcg
     4561 cccacctcgg tcgccctcgc ctggacgaag gacggcgccg atctcggccc ggtcgccggt
     4621 gcgctgcgcg ccaaccgggt cggcaactac cgctgccgcg cgaccggcac gaacgtcgcc
     4681 ggctcgaccg cggcgaccag tggagccgtg gcgatcttcg ccaccggcaa ggtccaggcg
     4741 aacaagaaga aagggaccgc ccgcctcacc gtgcggctgc ctgccgagcc cgggcggctg
     4801 gtgctggccg gcaaaggctt caaggccgtc gccaagcggg cctccggcaa aacgactgtg
     4861 ctgatcaaac cgaagggcgc gaagaaggcg aagctcgccg agcagggctc cctccgggcg
     4921 gtcgtcaagc tgacgtacac gccgccagac ggcctcgcag cgaccctgcg cacgcgcgtc
     4981 gcgctggtgc agggctaggc tccgccccaa tacggtgccg gtgtgagcaa cgtggagtac
     5041 ctgcaggaaa accagccgga gggggtgccc ccgctgccct tgacgggcga gc
//
LOCUS       DASRIG010000074         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_293583, whole genome shotgun sequence.
ACCESSION   DASRIG010000074 DASRIG010000000
VERSION     DASRIG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293583"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="VFB52_02000"
     CDS             complement(<1..1961)
                     /locus_tag="VFB52_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO05132.1"
                     /translation="MADSEQTRVGQYFDRHVGLLRALALIALVWGVGYLTWRVGWSGE
                     GANPVLFVMLLVTEIYGLYALATLAWFSWSRPKAVRPEPTPGRVVDVYVCTYDEPVEV
                     VMATLAGCSALTYPHTTYLLDDGRRPEMAELAAMAGAEYLVRGDNSHAKAGNINAALP
                     RTGGELVFILDADHVPMPDALDAIVGYFDDPQMAVVQSPHDFFNSDSVQHYSVGRHEQ
                     SLFYRVVCPGKNRHGAAYWCGSAALINREALLSIGGVATETIAEDFHTTIRLLRHGWR
                     SSYHDEVLVQGLAPHDLDGYLLQRDRWARGNLAVFTLPESPLRARTLSPLQRLSYLAS
                     LLAYLAPPMRLLLLLTLGAVLWTGALPMKISVVALAALWLPSVALNLAAGMALARGYM
                     RIPETAHYELLTMEIYTRALRCIVWPSKTAFKVTPKQGADGGGWTALRKLHLVLFCTV
                     LLAGGTLMRMLDLAGVGPLPDMPGIAAWIVVALGLIELRRLVKTSWTVGRRSQRRLVY
                     RFQGEAEAACYSGSGHTPARLLDASATGLGLATVAPLAVGDRPAVLLELDDAGGARRK
                     VAAQVEVRSCRPSEGEYVVGTTIVDIDPEARLALMEWCYVVCSHERLRGHRPAALQGP
                     QATIISLDAYREGAVRAPRPALPISLPAV"
     gene            2018..>2892
                     /locus_tag="VFB52_02005"
     CDS             2018..>2892
                     /locus_tag="VFB52_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05133.1"
                     /translation="MSRRGMTALVVVVFCAFVSSASAAVTIEEGANTVEAPNFKFNFA
                     DAAGNIERVDAIEWRREPALAFGPDIATAGGGACGDVSEFWGQSYGNADGQSPGPVVA
                     GNRGTWGARGQRSVEINSFSPTVCSGDTPPVPVRSRYSFFDGGAAANTVRLERSWNFA
                     AAQTHCCTVAQGMRAYVPRLPGGTYNQVVFPKADGSALNVLGTGGPAVQTDWNNVWVA
                     LNSSSTNAGLLILRDPVSVSAVPASVVTDNDLSSGSNNSGVSLDKPIGGWLLPVTEVE
                     YLCFYDEPSWPVANRL"
BASE COUNT          438 a         1009 c         1044 g          401 t
ORIGIN      
        1 tagaccgccg gcagggagat cggcagggcg ggacgtggcg cgcgtacggc gccctcgcgg
       61 taggcgtcga gggagatgat cgtcgcctgc gggccctgca gggccgccgg gcggtggccg
      121 cgcaggcgct cgtggctgca gacgacgtag caccactcca tcagtgccag gcgcgcctcg
      181 gggtcgatgt cgacgatcgt cgtgccgacg acgtattcgc cctcggaggg gcggcagctg
      241 cggacctcga cctgggcggc gactttgcga cgcgcgccgc cggcgtcgtc gagctcgagc
      301 aggaccgcgg ggcggtcgcc gaccgcgagc ggcgccacag tcgccagtcc cagcccggtg
      361 gcgctggcgt cgagcaggcg ggcgggggtg tggccggacc cggagtagca ggcggcctcg
      421 gcctcgccct ggaagcggta gacgaggcgg cgctggctgc gccgcccgac cgtccagctc
      481 gtcttcacca ggcgccgcag ctcgatcagc ccgagcgcga cgacgatcca ggcggcgatc
      541 cccggcatgt ccggcagcgg cccgacgccg gccaggtcga gcatccgcat caacgtcccg
      601 ccggccagca ggaccgtgca gaagaggacc aggtggagct tgcgcagcgc cgtccagccg
      661 ccgccgtcgg caccctgctt cggggtcact ttgaaggcgg tcttgctcgg ccagacgatg
      721 cagcgcagcg cccgcgtgta gatctccatc gtcagcagct cgtagtgcgc ggtctcgggg
      781 atccgcatgt agccgcgcgc cagcgccatc ccggcggcga ggttgagcgc caccgagggc
      841 agccagagcg ccgccagcgc gaccacgctg atcttcatcg gcagcgcccc ggtccagagc
      901 accgcgccga gcgtcagcag gagcagcagc cgcatcggcg gcgccaggta ggcgagcagg
      961 ctggccaggt aggaaagccg ctgcagcggg ctgagggtgc gggcccgcag cggcgactcg
     1021 ggcagggtga agacggcgag gttgccgcgc gcccagcggt cgcgctgcag caggtagccg
     1081 tcgaggtcgt gcggggcgag cccctggacc aggacctcgt cgtggtagct ggatcgccag
     1141 ccgtggcgca gcaggcggat cgtcgtgtgg aagtcctcgg cgatcgtctc ggtggcgacg
     1201 ccgccgatcg agagcagcgc ctcgcggttg atcagcgccg ccgagccgca ccagtaggcg
     1261 gccccgtggc ggttcttgcc cgggcagacg acgcggtaga acagcgactg ctcgtggcgg
     1321 ccgacggagt agtgctggac cgaatccgag ttgaagaagt cgtgagggga ttgaacgacg
     1381 gccatctgcg ggtcgtcgaa gtagccgacg atcgcgtcga gcgcgtcggg catcggcacg
     1441 tggtcggcgt cgaggatgaa gacgagttcg cccccggtgc gcggcaatgc cgcgttgatg
     1501 ttgcccgcct tcgcgtgcga gttgtcgccg cggacgaggt actcggcgcc ggccatcgcc
     1561 gccagctcgg ccatctccgg ccggcgaccg tcgtcgagca ggtaggtggt gtggggataa
     1621 gtgagcgcgc tgcagccggc cagcgtcgcc atcaccacct cgaccggctc gtcgtaggtg
     1681 cagacgtaga cgtcgaccac gcgccccggg gtcggctccg gccgcaccgc cttcggccgc
     1741 gaccaggaga accaggcaag ggtggcgagc gcgtagaggc cgtagatctc ggtcaccagc
     1801 agcatcacga acagcaccgg gttggcgcct tcgccgctcc agccgacccg ccaggtcagg
     1861 tagccgacgc cccaaaccag ggcgatcagc gccagcgcgc gcagcagccc gacatggcgg
     1921 tcgaagtact gcccgacgcg ggtctgctcg ctgtctgcca cgggaattga tcggcaaacc
     1981 cctgcgcccg gttgaggatc ggtggtagtt tcgctgcatg tctcgtcggg ggatgacggc
     2041 gctggtcgtc gtggtgtttt gcgcgttcgt cagttccgcc agcgctgcgg tgacgatcga
     2101 agagggcgcg aacaccgtcg aggcgccgaa cttcaagttc aacttcgccg acgccgccgg
     2161 caatatcgag cgggtcgacg cgatcgaatg gcggcgcgaa ccggcgctcg ccttcggccc
     2221 cgacatcgcc accgcgggcg gcggggcctg cggtgacgtc agcgagttct ggggccagtc
     2281 ctacggcaat gccgacggcc agtcacccgg cccggtggtc gccggcaacc gcggtacctg
     2341 gggcgcgcgc ggccagcgca gcgtcgagat caactccttc tcgccgacgg tctgctcggg
     2401 cgacacgccg ccggtcccgg ttcgcagccg ctacagcttc ttcgacggcg gcgcggcggc
     2461 gaacacggtt cgcctcgaac ggagttggaa cttcgccgca gctcagaccc actgctgcac
     2521 ggtggcccag ggaatgcgcg cctacgtgcc ccgtctcccg ggcggcacct acaaccaggt
     2581 cgtcttcccg aaagccgacg gcagcgccct caacgtgctc ggcaccggcg gtcccgccgt
     2641 gcagaccgac tggaacaacg tctgggtggc gctcaactcg tcgagcacca acgccggcct
     2701 gctgatcctc cgcgatcccg tcagcgtcag cgccgtcccc gccagcgtgg tcaccgacaa
     2761 cgacctctcc tcgggctcca acaactccgg cgtctcgctc gacaaaccga tcggcggctg
     2821 gctcctcccg gtcaccgagg tcgagtacct ctgcttctac gacgaaccca gctggccggt
     2881 ggccaaccgc ct
//
LOCUS       DASRIG010000075         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1597562, whole genome shotgun sequence.
ACCESSION   DASRIG010000075 DASRIG010000000
VERSION     DASRIG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597562"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFB52_02010"
     CDS             <1..791
                     /locus_tag="VFB52_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05134.1"
                     /translation="TNTVYRGTIEEPKGKHAEHGIPSVERIQKAIDRAGKHADISGAE
                     PTSTAGQPTYTVTASPSHDGGLLGGVELAWDAENGIPLRAAVYAADSGSPVLELEATD
                     VSFEAVDASVFEISPPEDAKVVELTPESADEHGKPREIVGADAVSKALDFELTAPKAL
                     AGLPRTGVRAIEVDGKQAALVTYGEGLGGIAVIQSATEPEKGNEATAESEELSLPEVV
                     IGDVKGEELDTALGSALWFSRAGVDYVVVGSVPPAAVEAAARGL"
     gene            803..1771
                     /locus_tag="VFB52_02015"
     CDS             803..1771
                     /locus_tag="VFB52_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05135.1"
                     /translation="MPRLMPQTTPIEVRGLVKRYGELLAVDNVDLTVEAGDVFGYLGP
                     NGAGKTTSLRMMLGLIRPTAGSVRLFGRDPQLSVSALEGVAGFVEAPSFYPYMNGRRN
                     LELFAALDAGDSAARIDESLEIVGLADRAKDKVGGYSHGMRQRLGIAAALLRQPRLLL
                     LDEPATGLDPGGMRDMRDLIRELSARGMTVLLSSHLLAEVEELCNRVAIVRRGRVAYQ
                     GSLADLRQQAGEGYRLRTTDEARARAVAVAQAGITEVVAGENATTFSARDEAAVSALS
                     LALAEAGALIVEMSPRHATLEDLFFRLTEGEESALASETRPKLRVA"
     gene            1768..>2486
                     /locus_tag="VFB52_02020"
     CDS             1768..>2486
                     /locus_tag="VFB52_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05136.1"
                     /translation="MNPGVLTIYRWELRKLRAQKRTYLGLGAAALVPLIFVIALVTQG
                     GQPEEVAFGRYVNETGLAIPLVLLLFGSVWMFPLITALVAGDIVSAEDRHDTLKGILT
                     RSVERQQVYAGKVLATLTYAVVAIAIMGAVALGAGILASGFNSITSLSGTEISANEGL
                     GLVGLSLLVYLLPIGAVACIGLLLSTVFRNSAAAIVGTLMFSLLVQLVGILPGLEAIR
                     PYLLSTQFDAWQGFLRSPIDW"
BASE COUNT          370 a          909 c          854 g          353 t
ORIGIN      
        1 cgaccaacac ggtctaccgc ggcacgatcg aggagccgaa aggcaaacac gccgagcacg
       61 gcatcccctc ggtcgagcgg atccagaaag cgatcgaccg ggccggcaaa cacgccgaca
      121 tctccggggc ggaaccgacc agcaccgccg gtcagcccac ctacacggtg accgcctcgc
      181 cgagccacga cggcggcctg ctcggcggcg tcgagcttgc ctgggacgcc gagaacggga
      241 tcccgctgcg cgccgcggtc tacgccgccg acagcggctc gccggtgctc gagctggagg
      301 cgaccgacgt gagcttcgaa gcggtcgacg cctcggtctt cgagatctcc ccacccgagg
      361 acgcgaaagt cgtcgagctg actccggagt ccgccgacga gcacggcaag ccgcgtgaga
      421 tcgtcggcgc cgacgccgtc tcgaaagcgc tcgacttcga gctgaccgcg ccgaaagcgc
      481 tggccggcct gccccgcacc ggggtccgcg cgatcgaggt cgacggcaag caggcggcgc
      541 tcgtcaccta cggcgaaggc ctcggcggga tcgccgtgat ccagtcggcg accgagccgg
      601 agaaaggcaa cgaagcgacc gccgagagcg aggagctgag cctgccggaa gtcgtgatcg
      661 gcgacgtcaa gggcgaagag ctcgacacgg cgctcggttc ggcgctctgg ttcagtcgcg
      721 ccggagtcga ctacgtggtc gtcggctcgg tgcctccggc cgcggtggag gccgcggcgc
      781 gcgggctctg aggcgaaggt agatgccgag gctgatgccg cagaccacgc cgatcgaggt
      841 ccgcggcctg gtcaagcgtt acggcgagct gctggccgtc gacaacgtcg acctcacggt
      901 cgaggccggc gacgtcttcg gctacctcgg ccccaacggg gccggcaaga cgacctcact
      961 gcgaatgatg ttgggactga tccggccgac ggccggatca gttcgtctgt tcgggcgcga
     1021 cccgcagctc agcgtcagcg ccctcgaggg cgtcgccggc ttcgtcgaag cgccctcgtt
     1081 ctacccgtac atgaacgggc gccgcaacct cgaactgttc gcggcgctcg acgccggcga
     1141 ctcggcggcg cggatcgacg agtcgctgga gatcgtcggg ctcgccgacc gcgccaagga
     1201 caaggttggc ggctactcgc acgggatgcg ccagcggctc gggatcgcgg cggcgctgct
     1261 gcggcagccg aggttgctcc tgctcgacga gccggcaacc gggctcgacc cgggcgggat
     1321 gcgcgacatg cgcgacctga tccgcgaact ctccgcccgc gggatgaccg tgctgctctc
     1381 cagtcacctg ctggccgagg tcgaagagct ctgcaaccgg gtcgcgatcg tgcgccgcgg
     1441 gcgggtcgcc taccagggct cgctcgccga cctgcgccag caggccggcg agggctaccg
     1501 gctgcggacc accgacgaag cccgcgcccg cgccgtcgcc gtcgcccagg cggggatcac
     1561 cgaggtcgtc gccggcgaga acgccaccac cttcagcgcc cgcgacgagg cggcggtcag
     1621 cgcgctctcg ctggcgctgg ccgaggccgg cgccctgatc gtcgagatga gcccgcgcca
     1681 cgcgaccctc gaggacctct tcttccgcct caccgagggc gaggagtccg cgctcgcctc
     1741 cgagacccgc cccaagctga gggtcgcgtg aacccgggag tgctgacgat ctaccgctgg
     1801 gagctgcgca agctgcgcgc ccagaagcgc acctacctcg ggctcggcgc cgccgcgctg
     1861 gtcccgctga tcttcgtgat cgccctggtc acccagggcg gccagccgga ggaagtcgcc
     1921 ttcggccgct acgtcaacga gaccgggttg gcgattcccc tcgtgctgct gctcttcggc
     1981 tccgtctgga tgttcccgct gatcaccgcg ctggtcgccg gcgacatcgt ctccgccgag
     2041 gaccgccacg acacgctcaa gggcatcctc acccgctcgg tcgagcgcca gcaggtctac
     2101 gccggcaagg tgctggcgac gctcacctac gcggtcgtcg cgatcgcgat catgggtgcg
     2161 gtcgcgctcg gcgccggcat cctcgcctcc ggcttcaact cgatcaccag cctctccggg
     2221 accgagatct cagccaacga agggctcggc ctggtcggcc tcagcctgct cgtctatctg
     2281 ctcccgatcg gcgccgtcgc ttgcatcggc ctgctgctct cgaccgtctt ccgcaacagc
     2341 gccgcggcga tcgtcggcac cctgatgttc tcgctgctgg tccagctggt cgggatcctg
     2401 cccgggctgg aggcgatccg gccctacctg ctgagcaccc agttcgacgc ctggcagggc
     2461 ttcctgcgct cgccgatcga ctggga
//
LOCUS       DASRIG010000076         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3431605, whole genome shotgun sequence.
ACCESSION   DASRIG010000076 DASRIG010000000
VERSION     DASRIG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3431605"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFB52_02025"
     CDS             <1..402
                     /locus_tag="VFB52_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO05137.1"
                     /translation="LDSGDLARADEDGYLWFFGRKKQVIVHDGSNISPYEVEGALVEH
                     PAIALAGAIGVHDEVHGENVRAYVTLEEGAEPPSRGDLIAFCRERIGYKAPEEIVFLD
                     EMPLNPTGKIDRVGLKRMAEDHLHPHGLEDG"
     gene            399..1226
                     /locus_tag="VFB52_02030"
     CDS             399..1226
                     /locus_tag="VFB52_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZO05138.1"
                     /translation="MSSRAVVFAGGGVAGISWMLGLVDGLIRAGVDLAGADLISGTSA
                     GSCVATNLATGAVAEACARQRRPSAEIVVPFDIDQFLDTVARHKREAPSEAEALVRIA
                     AMEPIGPSISPAERGAVIEARLPVHEWPERPLKAVCVDAGSGELLALDRDSGFGLVDA
                     VASSCALPGIWPPREVDGRTYVDGGIRSLSNIDVAAGHDRVLIVVPTALSDSVRASHQ
                     AEIAALGGAATHLISADEASLAALGSNPLDPDHREPALEAGLAQAPRELDALAAFWS"
     gene            1358..1930
                     /locus_tag="VFB52_02035"
     CDS             1358..1930
                     /locus_tag="VFB52_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05139.1"
                     /translation="MSRKALSDRNLNLAVVSAFAALVALAFLVFAGPAAAAGKKKADK
                     PWNNPNKAVEYKGTTASGHEITFTFKKNALWDMESGISVSCIPIQGSGPPSGGVELFD
                     YVGYVALNPQGEDFEFMKKVSVYYNEVTVKHTLATSINRKNGTIRGTQRMQFSYFLPQ
                     FPMPKFAIYSCLAEGSFVAKPVLSGGGKKK"
     gene            complement(1905..2528)
                     /locus_tag="VFB52_02040"
     CDS             complement(1905..2528)
                     /locus_tag="VFB52_02040"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05140.1"
                     /translation="MAGASPPTRPALRARYDARREQVVADAARIFAERGFQATSMAEL
                     TEATGLAAGGIYHYIDGKDDLLIAICDELLEPLLVQAREIVAGPGAPEQQLRALMRVW
                     LEHIASHLDHMLVFAQERHVIERERRWAGVRAQRKEFEQILDDVLARGVADGSMSFGD
                     RGLALRALLGMVNYSPQWLRPRGRLTPEQIADGYCDLLLATSSSRPR"
     gene            2600..>3657
                     /locus_tag="VFB52_02045"
     CDS             2600..>3657
                     /locus_tag="VFB52_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05141.1"
                     /translation="MDFELSDEHRLIQSTVRDFARQEVAPVAEELDREKRFPYEIVKG
                     LGALNLMGIPFPEEYGGGGGDSLAYALAVEELTRVDSSVAITLCAHTSLGTQPVYLFG
                     SEEQKAEWLPKLCAGEVLGAFGLTEPEAGSDAGNVRTTAKLEDGGEWVIDGAKQFITN
                     AGTDISGLVCITARTGPEEISNLIVPNGTPGYEQGEPYRKMGWNASDTRPLTFTDCRV
                     PEANLLGPRGKGFIQFLQVLDIGRIGVAAMGVGLAQGALDEALAYAKERRAFGQPISK
                     FQAIQGKLADMATEIEAARLLVYKAARQKDRGENFTLTAAQAKLKTGRLAVRATEEAV
                     QIHGGYGYIEEYPVCRFYR"
BASE COUNT          596 a         1304 c         1243 g          514 t
ORIGIN      
        1 ctggactcgg gcgatctcgc ccgggcagac gaggacggct acctctggtt cttcggccgc
       61 aagaagcagg tgatcgtcca cgacggctcc aacatcagcc cttacgaggt cgagggcgct
      121 ttggtcgagc acccggcgat cgccctcgcc ggcgcgatcg gcgtgcacga cgaagtccac
      181 ggcgagaacg tgcgcgccta cgtcaccctg gaggagggcg ccgagccgcc cagccgcggc
      241 gacctgatcg ccttctgccg tgagcggatc ggctacaagg cgcccgagga gatcgtcttc
      301 ctcgacgaga tgccgctcaa cccgaccggc aagatcgacc gggtcgggct gaagcggatg
      361 gccgaggacc acctgcaccc gcacgggctc gaggacggtt gagcagccgc gccgtcgtct
      421 tcgccggcgg cggtgtcgcc gggatctcct ggatgctcgg cctggtcgac gggctgatcc
      481 gcgccggggt cgacctggcc ggcgccgacc tgatcagcgg cacctcggcc ggctcctgcg
      541 tcgccaccaa cctggcgacc ggggcggtgg cggaggcctg cgcgcgccag cgccggccga
      601 gcgccgagat cgtcgtgccc ttcgacatcg accagttcct cgacacggtc gcccgccaca
      661 agcgcgaggc gccgagcgaa gccgaggcgc tggtccggat cgcggcgatg gagccgatcg
      721 ggccgtcgat cagcccggcc gaacgcggtg cggtgatcga ggcgcggttg ccggtccacg
      781 agtggcccga gcggccgctg aaggcggtct gcgtcgacgc cgggagcggc gagctgctgg
      841 cgctcgaccg cgactccggc ttcggcctgg tcgacgcggt cgcctcgagc tgcgcgctgc
      901 cggggatctg gccgccgcgc gaggtcgacg gccgcaccta cgtcgacggc gggatcaggt
      961 cgctgagcaa catcgacgtc gccgccggcc acgaccgcgt gctgatcgtc gtcccgaccg
     1021 cgctcagcga ctcggtccgc gccagccacc aggccgagat cgccgccctc ggtggcgccg
     1081 ccacccatct gatctctgcc gacgaggcct cgctcgccgc cctcggctcc aacccgctcg
     1141 accccgatca ccgcgagccc gccctggaag ccggcctcgc ccaagccccc cgcgagctcg
     1201 acgccctcgc cgccttctgg tcctgacccc cttagttagg ggttaggggg tcccggaaag
     1261 tttggggacg acctttgggg tcggccccga tgtgcgattg cgcttggcga cggaagctgg
     1321 gactcgttcg caaaccgcac ccactagggg gaagaaaatg agtcgcaagg cactctccga
     1381 tcgcaatttg aacctggccg tcgtctccgc gttcgcggcc ctggtcgcgc tggcgttcct
     1441 ggtcttcgcc gggcccgccg cggccgccgg caagaagaag gccgacaaac cgtggaacaa
     1501 cccgaacaag gcagtcgaat acaagggcac gaccgcctcg ggccacgaga tcaccttcac
     1561 cttcaagaag aacgccctct gggacatgga atccgggatc tcggtctcct gcatcccgat
     1621 ccagggcagc ggcccgccga gcggcggcgt cgaactgttc gattacgtcg gctacgtggc
     1681 gctgaacccc cagggcgagg acttcgaatt catgaagaaa gtctccgtct actacaacga
     1741 agtcaccgtc aaacacacgc tggcgacctc gatcaaccgc aagaacggga cgatccgcgg
     1801 cacccagcgg atgcagttct cctacttcct gccgcagttc ccgatgccga aattcgcgat
     1861 ctacagctgc ctggccgagg gctcgttcgt cgccaaaccc gtgctcagcg ggggcgggaa
     1921 gaagaagtag cgagcagcag gtcgcagtag ccgtcggcga tctgctccgg cgtgagccgg
     1981 ccccgcgggc gcagccactg gggtgagtag ttgaccatcc cgagcagcgc ccgcagggcc
     2041 aggccgcggt cgccgaagct catcgagccg tcggccacgc cgcgcgcgag cacgtcgtcg
     2101 aggatctgct cgaactcctt gcgctgggcg cgcacgccgg cccagcgccg ctcgcgctcg
     2161 atcacgtggc gctcctgggc gaagaccagc atgtggtcga ggtgggaggc gatgtgctcg
     2221 agccagacgc gcatcagcgc gcgcagctgt tgctcggggg cgcccgggcc ggcgacaatc
     2281 tcgcgcgcct ggaccagcag cggctcgagc agctcgtcgc agatcgcgat cagcaggtcg
     2341 tccttgccgt cgatgtagtg gtagatgccg ccggcggcga gcccggtcgc ctcggtcagt
     2401 tcggccatcg aggtcgcctg gaagccgcgc tcggcgaaga tccgggcggc gtctgcgacg
     2461 acctgctcgc ggcgcgcgtc gtagcgcgcc cgcagcgccg gtcgggtggg tggggaggcg
     2521 cccgccatcg gcgaaaaggt agcgcagaga ccgaacgccc cctcgaattt agaacgttca
     2581 ttcggtataa ctgccgacca tggacttcga gctaagcgat gagcaccgtc tgatccagtc
     2641 aaccgtgcgc gacttcgccc gccaggaggt cgcccctgtc gccgaggagc tggaccgcga
     2701 gaagcggttc ccctacgaga tcgtcaaggg cctcggcgcg ctcaacctga tggggatccc
     2761 gttcccggag gagtacggag gagggggcgg cgactccctc gcctacgcgc tcgcggtcga
     2821 ggagctgacc cgcgtcgact cctcggtggc gatcacgctc tgcgcccaca cctcgctcgg
     2881 cacccagccg gtctacctgt tcggcagcga ggagcagaaa gccgagtggc tgccgaagct
     2941 ctgcgccggc gaggtgctcg gcgccttcgg tttgaccgag ccggaagcgg gctcggacgc
     3001 cggcaacgtg cgcacgaccg cgaagctcga ggacggcggc gaatgggtga tcgacggcgc
     3061 caagcagttc atcaccaacg ccggcacgga catctccggg ctggtctgca tcaccgcccg
     3121 caccggcccc gaggagatct ccaacctgat cgtccccaac ggcacccccg gctacgagca
     3181 gggcgagccc taccgcaaga tgggctggaa cgcctccgac acgcggccac tcaccttcac
     3241 cgactgccgg gtccccgagg ccaacctgct cgggccgcgc ggcaaaggct tcatccagtt
     3301 cctgcaggtg ctcgacatcg gccggatcgg ggtcgcggcg atgggcgtcg gcctcgccca
     3361 gggggcgctc gacgaggcgc tcgcctacgc caaggagcgc cgggcgttcg gccagccgat
     3421 ctccaagttc caggcgatcc agggcaagct cgccgacatg gcgaccgaga tcgaggccgc
     3481 ccgcctgctc gtctacaaag ccgcccgcca gaaggaccgc ggcgaaaact tcaccctcac
     3541 cgccgcccag gcgaagctga agaccggccg gctcgccgtc cgcgcgaccg aggaggccgt
     3601 gcagatacac ggcgggtacg gctacatcga ggagtacccg gtctgccgct tctaccg
//
LOCUS       DASRIG010000077         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1092250, whole genome shotgun sequence.
ACCESSION   DASRIG010000077 DASRIG010000000
VERSION     DASRIG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092250"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFB52_02050"
     CDS             complement(<1..587)
                     /locus_tag="VFB52_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05142.1"
                     /translation="MRKRFAAGLLAAALGALALVPTGASAATEFGDSCVANDTVEAPI
                     TFVESSAPGNPLPTAAPVSGVLTHWRTNVIPVPFAIPATMKVLRLNAATLTASVVGEA
                     TGAALSGSNTFPVRLPIQAGDYLGISGGSQVGALICETAVDSGLVTMLNAGPGNSVPY
                     EAGSGEIRVPLVGVIEPDVDGDGYGDESQDGCPQSA"
     gene            complement(592..1539)
                     /locus_tag="VFB52_02055"
     CDS             complement(592..1539)
                     /locus_tag="VFB52_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05143.1"
                     /translation="MRKRALLGLAATAALVLSLAPAASAATEFGASCTANRAEEGMTP
                     VSLIGLTQNGVPSGAPVSGVVTSWKVRVIGTPSVFSQQLKVFRPTASPALFQVAGESA
                     PANVTGGENVAATRVPIQAGDLLGLFGAGDIGALYCEGPESAGAGNTIGGFAGNPPLG
                     SSATLIGSGSNVLLPARAVIEPDADKDGYGDETQDGCPQSALVQTACPVVVLDAFSIA
                     SGNAVKVLIATSTTAPVKVSGTVKLGKGSNAKLSAKQKTVTAGKITSFKLAFTGPLKE
                     KLKEIGPGQKLTLKITAKATNVAGQVSADKLQAKLKGQG"
     gene            complement(1544..2506)
                     /locus_tag="VFB52_02060"
     CDS             complement(1544..2506)
                     /locus_tag="VFB52_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05144.1"
                     /translation="MKMRKRIATAALSALGAMLLLAAPSASAAVEFGDNCVADDVAPD
                     TYTLTTLAAPPGPIPLTAPSAGVVTKIRSNVPAVLPFATPISVKILRNAGGNLYTAVN
                     QATVNAAPGISTSNVRLPVLAGDRLGLHGLPFSFEGTPIEGFSWYCDEMPGSTLGAAL
                     GDVPTGATAVFEGATDGRVPLVATLEPDADNDGFGDESQDLCPQGAAVQTACPAVTID
                     AYSLVGGNAVKVLVATSTQAPVKVTGTVKLGKGSKATLKGGTKGVPAGKLVRFTLKFP
                     GSLKEKLRELESNQKLTLKITASATNVAGQVSTDKLKAKLKGLG"
BASE COUNT          333 a          856 c          894 g          465 t
ORIGIN      
        1 gcgctctgcg ggcagccgtc ctggctctcg tcgccgtagc cgtcaccgtc gacgtcgggc
       61 tcgatcacgc caaccagcgg gacacggatt tcgccggacc cagcttcgta ggggacgctg
      121 ttgccggggc cggcgttcag catcgtcacg aggccgctgt cgaccgcggt ttcgcagatg
      181 agagcaccga cctgcgagcc accgctgatc cccaggtagt cgcccgcctg gatcggcagg
      241 cggaccggga aggtgttcga ccccgagagc gccgccccgg tcgcttctcc caccacgctc
      301 gccgtcagcg tcgcggcgtt gaggcgcagc actttcatcg tcgctgggat cgcgaacgga
      361 acgggaatca cgttggtgcg ccagtgggtc agcacgccgg agaccggcgc cgccgtcggc
      421 agcgggttgc cgggcgcgct cgactcgacg aaggtgatcg gcgcttcgac cgtgtcgttg
      481 gcgacgcagc tgtcgccgaa ttcggttgcc gccgaggcac ccgtgggcac cagcgcgagc
      541 gctcccagtg cggcagccag cagccccgcc gcgaatcgct ttctcatcgg tctagccctg
      601 tcccttcagc ttggcttgca gcttgtcggc gctgacctgg ccggcgacgt tggtcgcctt
      661 cgcggtgatt ttcagggtca gcttctgacc cggcccgatc tccttcagct tctctttcag
      721 cgggccggtg aaggcgagct tgaagctggt gatcttgccg gcggtgacgg ttttctgttt
      781 agcgctcagc ttcgcgttcg agcctttgcc gagcttcacc gtgccgctga ctttgaccgg
      841 cgccgtcgtg ctggtcgcga tcaggacctt gaccgcgttg ccgctcgcga tcgagaaggc
      901 atcgaggacg acgaccgggc aagcggtctg gaccagggcg ctctgcgggc agccgtcctg
      961 cgtctcatcg ccgtagccgt ctttgtcggc gtcgggctcg atcacggccc gggccgggag
     1021 caggacgttg ctgccggatc cgatcagcgt cgcactcgag ccgagcggtg ggttgccggc
     1081 gaagccgccg atcgtgttcc ccgccccggc ggattcgggc ccttcgcagt acagcgcgcc
     1141 gatgtcgccg gcgccgaaga ggccgagtag gtcgccggcc tggatcggga cccgggtcgc
     1201 tgcgacgttt tccccgccgg tcacgtttgc gggagcgctt tcgccggcaa cctggaacag
     1261 cgctgggctc gcggtcggcc ggaacacctt cagctgctgt gagaacacgg acggcgtccc
     1321 gatcactctg accttccagc tcgtgaccac cccggagacc ggcgcgcccg aggggacgcc
     1381 gttctgggtc agcccgatca ggctcacggg cgtcatccct tcttcggccc ggttcgcggt
     1441 gcagctggca ccgaattcgg ttgccgccga agccgccgga gcgagcgaga ggaccagcgc
     1501 cgcggtcgcg gcgagtccga ggagagcgcg ctttctcatt gtgttacccc agtcccttca
     1561 gcttcgcctt gagcttgtcg gtgctgacct ggccggcgac gttggtggcg ctggcggtga
     1621 ttttcagggt cagcttctga ttcgactcca gctccctcag cttctccttc agggagccgg
     1681 ggaacttcag cgtgaagcgc acgagtttgc cggcgggtac gcctttggtg ccgcctttga
     1741 gggtggcctt cgagcctttg ccgagcttca ccgtgccggt caccttcacc ggagcctggg
     1801 tgctggtcgc gaccaggacc ttgaccgcgt tgccgccgac cagcgagtag gcgtcgatcg
     1861 tcaccgccgg gcaggccgtc tggaccgcgg cgccctgcgg gcagaggtcc tggctctcgt
     1921 cgccgaagcc gtcgttgtcg gcgtcgggct cgagcgtggc gacaagcggg acccgcccgt
     1981 cggtggcgcc ttcgaagacg gcggtcgcgc cggtgggaac atcgcccaag gcggccccga
     2041 gggtgctgcc gggcatttcg tcgcagtacc agctgaagcc ttctatcggc gtgccttcga
     2101 agctgaaggg caggccgtgc aagccgagcc ggtcgcccgc gaggaccggc agacgcacgt
     2161 tgctggtcga gatcccgggg gcggcgttga ccgtcgcctg gttgacggcg gtgtacagat
     2221 tgccaccggc gttgcgaagg atcttgacgc tgatcggggt cgcgaacggg aggacggccg
     2281 gcacgttcga gcggattttg gtcaccaccc ccgcgctggg cgcggtcagc gggatcggcc
     2341 cgggaggcgc cgccagcgtg gtgagcgtgt aggtgtctgg cgcgacgtcg tcggcgacgc
     2401 agttgtcgcc gaattcgacc gcggccgagg cgctcggcgc ggccagcagc agcatcgctc
     2461 ccagggccga cagggcggca gtcgcgatcc gcttcctcat cttcatcctc gtcccttcag
     2521 cttggcttgc agcttgtcgg tgctgacc
//
LOCUS       DASRIG010000078         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2651841, whole genome shotgun sequence.
ACCESSION   DASRIG010000078 DASRIG010000000
VERSION     DASRIG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651841"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFB52_02065"
     CDS             complement(<1..1067)
                     /locus_tag="VFB52_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZO05145.1"
                     /translation="MSERDTEAGRQGLTRKELIGSGAGAAAAALLAGSGVDRAVAAAK
                     GGGKSVAGMNMILILTDQERAIQHFPKNWLRKNLPGMRRLRQHGLSFERAFTNACMCS
                     PARSTLMTGFFPAQHGVKYTLEEDMPYPEYPLQAEMPRPPELTNPATVMRAAGYTPVY
                     KGKWHCSKPATKEDAKPSDLARYGWDRWNPPDAGANQTVPEAGGGYIDNDGRFIESVG
                     STEEGKEGVMQYLSSVAAKQQPFFLVISLVNPHDVLFFPSKTFEEAGYDKSWIRGELE
                     PPATAEESLRTKPTVQEEFRNIFNLTGKPKGPIQQAQYLNFYGNLMRSSDNYIVDVLN
                     KLEETGLYDDTLIVRTADHGE"
     gene            complement(1064..2461)
                     /locus_tag="VFB52_02070"
     CDS             complement(1064..2461)
                     /locus_tag="VFB52_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZO05146.1"
                     /translation="MADVTRSARLPLVLAALVALAALLPAGASAAKPGPGTLTQLQGK
                     KGCIVDRSSKPGTCASGRGLKGPGPFMGSRAVAVSPDGRNVYVASATSNAIAIFQRDP
                     KSGALTQPKGVSGCVAVGGKEGCRKVAAFNGPNSVAVSPDGRNVYATARNSSTLLSFT
                     RNPKTGYLRPIPGFACSAGVAIPACTPANGLVGPDVVIVSPDGRNVYAGAFFGSAVVA
                     FSRDPSTGVLTQLAGTAGCIAAATAGCTTAIGISSIEGLAISGDGANVYAAAAASNAL
                     TELARDPSTGALSQLPAPNSCIYNAPITGCTAGRELGGPNAVAVAPGGENVYVTSFLS
                     NSVTAFDRTPGSGVLAQKEATNACLIFLRSAGCSFGRAMDAPEGLDVSPDGQNIYIAS
                     IQTGAIDVLSRNGKGAIRQLAGTAGCVARKQVQGCTLGRAVSGVSSVAVSPDGRNVYA
                     TANESNAIDVFRRNR"
     gene            complement(2454..>3175)
                     /locus_tag="VFB52_02075"
     CDS             complement(2454..>3175)
                     /locus_tag="VFB52_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05147.1"
                     /translation="STSGAFKYTTYKVRLSPETETGMYEMSNPKTTRQAKTGFVIPSQ
                     DEWIKAAYYDPNGGGTYSYWKYPTNPGKPGEGMSDGPKQAQLDANGNVANAAEQPVSI
                     FHMTGTEPPTAAPYWCPSNQTAEACNTANPWGIEPQAYDKAFAGSLGTVGTALTLSPW
                     GALDMGGNAVEWTDTITPPPFGVKGARVWRRLHGGIANAPVYQLWISAVGLQPQDNSF
                     FTATYPWLGIRIGVIGNLKNG"
BASE COUNT          401 a         1015 c         1180 g          579 t
ORIGIN      
        1 atctcgccgt ggtcggcggt gcgcacgatc agggtgtcgt cgtagaggcc ggtctcttcg
       61 agtttgttga gcacgtcgac gatgtagttg tccgaggagc gcatcaggtt gccgtagaag
      121 ttcaggtact gggcctgctg gatcggccct ttcggcttcc cggtgaggtt gaagatgttg
      181 cggaattctt cctgcaccgt gggcttcgtc cgcaggcttt cttccgcggt cgcaggtggt
      241 tcgagttcgc cccggatcca ggatttgtcg tagccggctt cctcgaaggt tttgctcggg
      301 aagaagagga cgtcgtgcgg gttgaccagc gagatcacca ggaagaacgg ctgctgtttg
      361 gccgcgaccg agctgaggta ctgcatcacg ccctctttgc cttcctcggt cgagccgacg
      421 gattcgatga agcgcccgtc gttgtcgatg tagccgccgc cggcctcggg caccgtctgg
      481 ttggcgccgg cgtccggcgg gttccagcgg tcccacccgt agcgggccag gtcggagggc
      541 ttggcgtctt ctttggtcgc cggcttcgaa cagtgccact tgcccttgta gacgggggtg
      601 tagccggcgg cccgcatcac cgtggccggg ttggtcagtt cgggcggccg cggcatttcc
      661 gcctgcagcg ggtattccgg gtagggcatg tcttcctcga gcgtgtactt gactccgtgc
      721 tgcgccggga agaagccggt catcagggtc gagcgggccg gcgagcacat gcaggcgttg
      781 gtgaaggcgc gttcgaagct gagtccgtgc tggcgtagcc gccgcatccc cggcaggttc
      841 ttacgcagcc agttcttcgg gaagtgctgg atcgcgcgct cctggtcggt gaggatcagg
      901 atcatgttca tcccggcgac gctcttgccg ccgcccttgg cggcggcgac cgcgcggtcg
      961 accccggacc cggcgagcag cgccgcggcg gcggcccccg ccccgctccc aatcagctct
     1021 ttgcgggtca ggccctggcg cccggcctcg gtgtcgcgct cactcatcgg ttcctcctga
     1081 agacatcgat cgcgttgctt tcgttggcgg tcgcgtagac gttgcgaccg tcgggactga
     1141 ccgccaccga gctgaccccg gagaccgcgc ggccgagcgt gcagccctgg acctgcttgc
     1201 gggcgacgca gccggcggtc ccggccagct gtcggatcgc gcccttgccg ttgcgggaga
     1261 gcacgtcgat cgcgccggtc tggatcgagg cgatgtagat gttctggccg tcgggcgaga
     1321 cgtcgagtcc ctccggcgcg tccatcgcgc ggccgaacga acagccggcg gagcgcagga
     1381 agatcaggca ggcgttggtc gcttccttct gcgccagcac gccgctgccg ggggtgcggt
     1441 cgaaggcggt gacgctgttg gagaggaacg aggtgacgta gacgttttcc ccgcccgggg
     1501 cgaccgcgac cgcgttcggc ccgccgagct cacgccctgc cgtgcagccg gttatcggcg
     1561 cgttgtagat gcaggagttg ggggcgggca gctggctgag cgcgccggtc gagggatcgc
     1621 gggcgagctc ggtcagggcg ttggaggccg cagccgccgc gtagacgttg gcgccgtcgc
     1681 cgctgatcgc caggccctcg atcgagctga tcccgatcgc ggtggtgcag ccggcggtcg
     1741 ccgcggcgat gcagccggcg gtcccggcga gctgggtcag gaccccggtc gaggggtcgc
     1801 gggagaaggc gacgaccgcg ctgccgaaga aggcgccggc gtagacgttg cggccgtcgg
     1861 ggctgacgat cacgacgtcg gggccgacca gcccgttggc gggcgtgcag gcgggtatcg
     1921 cgaccccggc cgagcaggca aatccgggga tcggccgcag gtagccggtt ttcgggttgc
     1981 gggtgaagct gagcagcgtg ctgctgttgc gggcggtcgc gtagacgttg cgcccgtcgg
     2041 ggctgactgc gaccgagttc ggcccgttga aggcggcgac tttgcggcag ccttccttgc
     2101 cgccgaccgc gacgcagccg ctgacgccct tcggctgggt gagcgcgccg gatttcgggt
     2161 cgcgctggaa gatcgcgatc gcgttgctgg tcgccgaggc gacgtagacg ttgcggccgt
     2221 cggggctgac cgccaccgct cgcgagccca tgaacggacc cgggcccttc agcccgcgac
     2281 cgctcgcaca ggtgcccggt ttcgaggagc ggtcgacgat gcagcccttc ttgccctgca
     2341 gctgggtcag cgtgccgggc cccggcttcg ccgccgacgc gcccgccggc agcagcgccg
     2401 ccagcgcgac gagggccgcg aggacgagcg gcaagcgggc gctgcgggtc acgtcagcca
     2461 ttcttcaggt tgccgatgac accaatccgg attcccagcc acgggtaggt ggcggtgaag
     2521 aacgagttgt cctgcggctg caggccaacc gcggagatcc agagctggta gacgggggcg
     2581 ttggcgatcc cgccgtgcag gcggcgccag acccgcgcgc ctttgacgcc gaacggcggc
     2641 ggggtgatcg tgtcggtcca ctcgaccgcg ttgccgccca tgtccagcgc gccccagggc
     2701 gagagggtga gcgccgtgcc gacggtgccg aggctgccgg cgaaggcttt gtcgtaggcc
     2761 tgcggttcga tcccccacgg gttcgccgtg ttgcaggctt ccgccgtctg gttggacggg
     2821 caccagtagg gcgccgccgt cggcggttcg gtcccggtca tgtggaagat cgagaccggc
     2881 tgttcggcgg cgttggcgac gttgccgttc gcgtcgagct gcgcctgctt cggtccgtcg
     2941 ctcatccctt ccccaggttt gcccgggttg gtcgggtact tccagtagga gtaggtgccg
     3001 ccgccgttgg ggtcgtagta ggcggccttg atccactcgt cctggctcgg gatcacgaac
     3061 ccggtcttgg cctggcgggt cgtcttcggg ttgctcattt cgtacatgcc cgtctcggtt
     3121 tcgggcgaga gccgcacctt gtaggtcgtg tacttgaacg ccccggacgt gctct
//
LOCUS       DASRIG010000079         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2120075, whole genome shotgun sequence.
ACCESSION   DASRIG010000079 DASRIG010000000
VERSION     DASRIG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120075"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFB52_02080"
     rRNA            <1..203
                     /locus_tag="VFB52_02080"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            272..387
                     /gene="rrf"
                     /locus_tag="VFB52_02085"
     rRNA            272..387
                     /gene="rrf"
                     /locus_tag="VFB52_02085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            400..639
                     /locus_tag="VFB52_02090"
     CDS             400..639
                     /locus_tag="VFB52_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05148.1"
                     /translation="MSRPSGRLVVFLGRYALASAAPAALGTRGGEEGFGEAFLRRSLA
                     TAFAGPGSAPNGLEPVLTEGGEVDREVDGVGQAVG"
     gene            complement(913..1836)
                     /locus_tag="VFB52_02095"
     CDS             complement(913..1836)
                     /locus_tag="VFB52_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO05149.1"
                     /translation="MGRGWKIALGVVVVLVSLLAINTLLVDGDTDAAALTVPGGRILD
                     LPGGDVQVVEGGPRNGSPIVLIHCFSCALDYWDGMRPALERTHRVVAIDLRGHGGSEK
                     PGSGYAMPEQASLVAEALRELQVSDAAVVGHSLGGAVSVALAESAPKLVDRVAIVDTP
                     PDDTYGDLGFLAGLGFQPVLGEALWTIKPDFSVRQGLEVAFAPGFDIPDAFVDDVNQL
                     TYTAYDESPTYIEDYLGEESLDRRLRATGKPLMVLMGAEEQVVDDPERALAQYAGGVP
                     GAETHLIAGAGHSPNVEKPALTAGLVLKFAN"
     gene            1862..3157
                     /gene="murA"
                     /locus_tag="VFB52_02100"
     CDS             1862..3157
                     /gene="murA"
                     /locus_tag="VFB52_02100"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZO05150.1"
                     /translation="MQKFVIQGGAPLSGELTVAGNKNAALPILAACLLTEEDLLLHRV
                     PRIRDTEAQIALLERLGVEADWVGDNSVRLCASGVTDPSVDEELSGKIRASFLVAGPL
                     LARFGEAHMPPPGGDFIGRRRLDAHLDAFKDLGAVVAGDRWIELTAPGGLCPCEIFMD
                     EPSVMGTENALMAAALTPGATRISNAASEPHVQDLARLLTKMGARVDGIGSNVMTVNG
                     SDKLGGAEHTISPDHIEVGSFMALAAATGGELRIRDVEPLDLMATRRHFRRLGLRSEI
                     EGKDVIVPPEQELKIEMDLGDAIPKIEDGPWPAFPADLTSIALALATQAEGEILIFEK
                     MFENRLFFVDKLVAMGARITLCDPHRAIVSGPSRLHGERVESPDIRAGMAMLIAALAA
                     DGTSEIGNIRQIDRGYEQIDERLRALGAQIERVSAEVPA"
     gene            3194..4186
                     /gene="hisZ"
                     /locus_tag="VFB52_02105"
     CDS             3194..4186
                     /gene="hisZ"
                     /locus_tag="VFB52_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZO05151.1"
                     /translation="MIHPIPPGSRDILPDEMRELRKLNRALIEVFEERGYGEVATPAI
                     EYDEVLERGDSRGVASAYRFFDENGETLALRSDMTVPIARLAASRYADAETPLRLCYL
                     ASLYRAVRPQRGQMREFVQAGVELIGAGAPDGTVEVIEVLEAALDAAGLDRAVIGLGD
                     ADLYRQLLTEFGVEGEERDSLLARLAAHDIVGLENELTALPGISDEQAATCLALSQLR
                     GGSEVLEEARKLGGEAVQRATAQLQATYEALAARGAADRVQIDLGLLRDLGYYSGAIL
                     EVYDPALGHVLGGGGRYDGLMKKFGLDLPAAGFALYLERVHVAQMEEQRRGRAS"
     gene            4183..4857
                     /gene="hisG"
                     /locus_tag="VFB52_02110"
     CDS             4183..4857
                     /gene="hisG"
                     /locus_tag="VFB52_02110"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZO05152.1"
                     /translation="MSSFAGSRARPDGPLKLAVPRGALFGETLDVLDRAGIDTSELRG
                     DSRSLIFEADGLTLVTMRPSDVPTYVEAGAADVGITGKDVLLENPDRAVYELLDLGYG
                     RCRMVLAGKVGDTRLGEAQRRLGMMRIATKYPRTAERHFESTGRQVDLIEVKGSVELA
                     PLIGLGDGIVDLVATGRTLEENDLEVREEIVVCTARFVANRVAHKLRAAEVDDLTARL
                     REAVGG"
     gene            4854..>6028
                     /gene="hisD"
                     /locus_tag="VFB52_02115"
     CDS             4854..>6028
                     /gene="hisD"
                     /locus_tag="VFB52_02115"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZO05153.1"
                     /translation="MRVRHFAWGGEAQAVADEIRLWASELSDPVDVAPIGQAVLENGD
                     AAVLDLTNRFDATEAPLSTSLVAPESAQGALDDLDPELREALDVAAANVRAVASAQLA
                     APLPVELPQGQTVTVREVPVGAAGIYAPGGRAAYPSSVLMCAIPARAAGVGRLVLASP
                     PGPDGKLHPLVLAAAALCEVDEIYAIGGAQAIFALAYGTETIGAVDVIAGPGNAWVRE
                     AKRAVYGSVGIDSLAGPSELMLIAGHDADPEWAALDLCAQAEHGADSPLVVVAVEEHV
                     LDAIAAATERAAAERPSVADAPLALVQVTEIEQAIALANAYAPEHLELDEEDAALMAE
                     RVITAGCVFVGRYGATAFGDYVAGSNHVLPTGGAGRFSGPLGPGVFRRRVATVELDA"
BASE COUNT          901 a         2115 c         2123 g          889 t
ORIGIN      
        1 tcctgccaag ggcatcgcag gttagctacg cacggaacgg ataaccgctg aaagcatcta
       61 agcgggaagc cgtcctcgag atgaactctc ccatcccctt gagggagtaa gacccctggt
      121 agaccaccag gttgataggc cagcggtgga agcgtggtaa cacgcgtagc cgactggtac
      181 taatgggtcg agggcttgac ggatatttta agtacccatt gcggctttgt gcggttttca
      241 agctccaccg aggcggttgt ctcgcaaaat tccggtggcg catagcgaga gggaaacacc
      301 tcttcccatt ccgaacagag cagttaagcc tttcagcgcc gatggtactt ggggggcaac
      361 cccctgggag agtaggtcgc cgccggttct tcgtacgact tgagccgccc ttcggggcgg
      421 ctcgtcgtct ttctaggccg ctacgccctc gcgagcgccg cgccggcggc gctgggcaca
      481 cgaggtgggg aagagggctt cggcgaagct ttcctgcgcc ggtcgctcgc gaccgccttc
      541 gcaggcccgg ggagtgctcc caacgggctc gagcccgtac tcaccgaggg tggcgaagtc
      601 gatcgcgagg tcgacggcgt cggccaggcg gttggttagc gtttgcttag aatcgaacat
      661 gggttcgtac ggtacgccgg tcctcggacg gcaactcgtt cctgcaagtc tacttgcaat
      721 tgggccgcgg gaatggcttg ctagagcgga ttacggcgaa cgcgagcacg ccgcatcgct
      781 actggcgaca gtagcgatgc gaaccccaga agcgcgaact gcgaatgttt aggcgtccct
      841 atagagcttt cgactgcagc cggtggctgc ggctctattc gcctgtgtga cggatcttac
      901 gcaagaccgg acctaatttg caaatttcag cacgaggcca gcggtcagag cgggtttctc
      961 gacgttcggc gagtggccgg cgcccgcgat cagatgggtc tcggcgcccg ggacgccgcc
     1021 ggcatactgg gccagcgcgc gctccgggtc gtcgaccacc tgctcctcgg cgcccatcag
     1081 aaccatcaat ggctttccgg ttgcccgcag gcgccggtcg agcgactctt ccccgaggta
     1141 atcctcgatg taggtcggcg actcgtcgta ggcggtgtag gtgagctggt tgacgtcgtc
     1201 gacgaaggcg tcggggatgt cgaagccggg cgcgaaggcg acctcgagcc cctggcggac
     1261 cgagaagtcg ggcttgatcg tccacagggc ctcgccgagg accggctgga agccgaggcc
     1321 ggcgaggaag ccgaggtccc cgtaggtgtc gtcggggggc gtgtcgacga tcgcgacccg
     1381 gtcgaccagc tttggggccg attcggccag ggccacgctg accgcgccgc ccagcgagtg
     1441 gcccacgacc gcggcgtcgc tgacctgcag ctcgcgcagc gcttccgcaa ccaggctcgc
     1501 ctgctcgggc atggcgtagc cggagccggg cttttccgag ccgccgtggc cgcgcaggtc
     1561 gatcgcgacg acccggtgag tccgttccaa tgccggccgc atcccgtccc agtagtcgag
     1621 cgcgcagctg aagcagtgga tcagcacgat cgggctgccg ttgcggggcc cgccttcgac
     1681 cacctgcacg tcgcccccgg ggagatcgag gatccggccc ccgggcacgg tgagcgcggc
     1741 cgcgtcggta tctccatcga ccaggagggt gttgatcgcg agcagcgaaa ccagcaccac
     1801 gaccaccccc aaggcgatct tccagcctct ccccatgccg ctcgttaccc tagctcagcc
     1861 gatgcagaaa ttcgtaatcc aaggcggcgc gccgctctcc ggagagctga ccgtcgccgg
     1921 caacaagaac gcggccctgc cgatcctcgc tgcctgcctg ctcaccgagg aggatctgct
     1981 gctgcacagg gtgccgcgga tccgcgacac cgaagcccag atcgcgctgc tggagcggct
     2041 cggggtcgaa gccgactggg tcggcgacaa cagcgtgcgg ctctgcgcct ccggcgtcac
     2101 cgatccctcg gtcgacgagg agctctcggg caagatccgc gcctcgttcc tggtcgcggg
     2161 cccgctgttg gcccgcttcg gcgaggccca catgccgccc ccgggcggag acttcatcgg
     2221 ccgacgccgg ctcgacgccc acctcgacgc cttcaaggac ctcggcgcgg tggtcgccgg
     2281 cgaccgctgg atcgagctga ccgcgcccgg cggcctctgc ccctgcgaga tcttcatgga
     2341 cgagccctcg gtgatgggca ccgagaacgc gctgatggcg gcggcgctga cccccggcgc
     2401 gacccgcatc tccaacgccg cctccgagcc ccacgtccag gacctggccc gcctgctgac
     2461 caagatgggc gcccgcgtcg acgggatcgg ctccaacgtg atgaccgtca acggctccga
     2521 caaactcggc ggcgccgagc acacgatctc gcccgaccac atcgaggtcg gcagcttcat
     2581 ggctctggcc gcggcgaccg ggggggagct gcggatccgc gacgtcgagc cgctcgacct
     2641 gatggccacc cggcgccact ttcgccgcct cggcctgcgc tcggagatcg agggcaagga
     2701 cgtgatcgtg ccgcccgaac aggaactgaa gatcgagatg gacctcggcg acgcgatccc
     2761 gaagatcgag gacgggccct ggccggcctt cccggccgac ctcacctcga tcgcgctggc
     2821 cctggcgact caggccgagg gtgagatctt gatcttcgag aagatgttcg agaaccgtct
     2881 gttcttcgtc gacaagctgg tcgcgatggg cgctcggatc acgctttgcg atccccacag
     2941 ggcgatcgtc agcggtccga gccggcttca cggtgagcgc gtcgagagcc ccgacatccg
     3001 cgccggcatg gcgatgctca tcgccgccct ggccgccgac ggcacctcgg agatcggcaa
     3061 catccgccag atcgaccgcg gctacgagca gatcgatgag cgcctgcgcg cgctgggagc
     3121 ccagatcgaa cgcgtttctg ccgaggttcc ggcatagcgc ttcccgccgc ggttctctcg
     3181 taaggtcaat ccgatgatcc acccgatccc gcctggaagc agggacatcc tgcccgacga
     3241 gatgcgcgag ctgcgcaagc tcaaccgtgc cctgatcgag gtcttcgaag agcgcggcta
     3301 cggcgaggtg gcgacgccgg cgatcgagta cgacgaggtg ctcgagcgcg gcgacagtcg
     3361 cggtgtcgcc agcgcctacc gcttcttcga cgagaacggc gagacgctgg ccctgcgctc
     3421 cgacatgacc gtgccgatag cccgcctggc cgcgagccgc tacgccgacg ccgagacgcc
     3481 cttgcgcctc tgctacctgg ccagcctcta ccgggcggtg cgcccgcagc gcggccagat
     3541 gcgcgagttc gtccaggccg gggtcgagct gatcggcgcc ggcgcgccgg acggcaccgt
     3601 cgaggtgatc gaggtcttgg aggcagcgct cgacgccgcc ggcctcgacc gcgccgtgat
     3661 cgggctcggc gacgccgatc tctaccgcca gctgctgacc gagttcgggg tcgagggtga
     3721 ggagcgcgac tcgctgttgg cgcggctggc cgcgcacgac atcgtcggcc tcgagaacga
     3781 gctgaccgcg ctgccgggga tcagcgacga gcaggcggcg acctgcctgg cgctctcgca
     3841 gctgcgcggt ggcagcgagg tcctcgagga ggcgcgcaag ctcggcggcg aggcggtcca
     3901 gcgggcgacc gcgcagctgc aggcgaccta cgaggcgctg gccgcgcgtg gcgccgccga
     3961 tcgcgtccag atcgacctcg gcctgctgcg cgacctcggc tactacagcg gcgcgatcct
     4021 cgaggtctac gacccggcgc tcggccacgt gctcggcggc ggtgggcgct acgacggctt
     4081 gatgaagaag ttcggcctcg acctgccggc ggccgggttc gcgctctacc tggagcgcgt
     4141 gcacgtcgcc cagatggagg agcagcggcg ggggagggca tcgtgagctc ctttgccggt
     4201 agccgcgccc ggcccgacgg gccgctgaag ctggcggtgc cccgcggggc gctgttcggg
     4261 gaaacgctgg acgtgctcga ccgcgccggg atcgacacct ccgagctgcg cggcgactcg
     4321 cgctcgctga tcttcgaggc cgacggcctg accctggtga cgatgcgccc ctccgacgtg
     4381 ccgacctacg tcgaggcggg cgccgccgac gtcgggatca cgggcaagga cgtgctgctc
     4441 gagaaccccg accgggccgt ctacgagctg ctcgacctcg gctacgggcg ctgccgcatg
     4501 gtgctcgccg gcaaggtcgg cgacacacgc ctgggcgaag cccagcgccg cctcgggatg
     4561 atgcggatcg cgaccaagta cccgcgcacg gccgagcgcc acttcgagtc gaccggccgc
     4621 caggtggacc tgatcgaggt caagggctcg gtcgagctgg cgccgctgat cgggctcggc
     4681 gacgggatcg tcgacctggt cgcgaccggc cgcactctgg aagagaacga cctcgaggtg
     4741 cgcgaggaga tcgtcgtctg cacggcccgc ttcgtcgcca accgggtcgc ccacaagctg
     4801 cgcgccgccg aagtcgacga cctgaccgcg cgcctgcggg aggcggttgg gggatgaggg
     4861 tgcggcactt cgcatggggc ggcgaggccc aggcggtcgc cgatgagatc cgcctctggg
     4921 cctcggagct gagcgacccg gtcgacgtgg cgccgatcgg ccaggccgtg ctcgagaacg
     4981 gcgacgccgc ggtgctcgac ctgaccaacc gcttcgacgc gaccgaggcg ccgctgagca
     5041 cctccctggt ggcgcccgag agcgcccagg gggcgctcga cgacctcgac ccggagctgc
     5101 gcgaggcgct cgacgtcgcc gccgccaacg tgcgcgccgt cgcctcggcg cagcttgccg
     5161 cgccgctgcc ggtcgagctg ccgcagggcc agaccgtgac tgtgcgcgaa gttccggtcg
     5221 gcgcggcggg gatctacgcg cccggcgggc gcgccgccta tccctccagc gtcttgatgt
     5281 gcgcgatccc ggcccgcgcg gccggagtgg ggcggctggt gctcgcctcg ccgcccgggc
     5341 ccgacggcaa gctgcacccg ctggtcctgg ccgccgcggc gctctgcgag gtcgacgaga
     5401 tctacgcgat cggcggcgcc caggcgatct tcgcgctcgc ctacgggacc gagacgatcg
     5461 gggcggtcga cgtgatcgcc ggccccggca acgcctgggt gcgcgaggcc aagcgcgccg
     5521 tctacggatc ggtcgggatc gactcgctgg ccgggccctc ggagctgatg ctgatcgccg
     5581 gccacgacgc cgaccccgag tgggcggcgc tcgacctctg cgcgcaggcc gagcacggcg
     5641 ccgacagccc gctggtcgtc gttgcggtcg aggagcacgt cctcgacgcg atcgccgcgg
     5701 ccaccgagcg cgccgccgcc gagcggccca gcgtcgccga cgcgccgctg gcgctggtcc
     5761 aggtgaccga gatcgagcag gcgatcgcgc tcgccaacgc ctacgcgccc gagcacctcg
     5821 agctcgacga ggaggacgcg gcgctgatgg ccgagcgggt gatcaccgcc ggctgcgtct
     5881 tcgtcggccg ctacggcgcg accgccttcg gcgactacgt cgccggctcc aaccacgtgc
     5941 tcccaaccgg cggcgccggc cgtttctccg gcccgctcgg gccgggcgtc ttccgccgcc
     6001 gcgtcgcgac cgtcgagctc gacgcgga
//
LOCUS       DASRIG010000080         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3692704, whole genome shotgun sequence.
ACCESSION   DASRIG010000080 DASRIG010000000
VERSION     DASRIG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3692704"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFB52_02120"
     CDS             <1..286
                     /locus_tag="VFB52_02120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO05154.1"
                     /translation="AACAGVTLRAVATSLGIAVESGSVRAEGDLDFRGTLAVDREAPV
                     GFRAIRLHIEVESAASEEELATLLKLTERYCVVLQTIAGAPDLSASISSA"
     gene            complement(287..1534)
                     /locus_tag="VFB52_02125"
     CDS             complement(287..1534)
                     /locus_tag="VFB52_02125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO05155.1"
                     /translation="MKFNLFLLPTIPASDETRRELRPIGRNTEHYQEMLTEVRDLTKL
                     AEEVGFDSIGLTEHHLHSEGWEVSVAPLMLFADLAARTEKIKFLTLGLGATTWDPIRL
                     AEETAILDQLCQGRYYAGFARGYQDRWVKILGQQYHVGGTLSDGSAKDLKNREIYEEV
                     VELVYKAWTEDALTHDGEHYKVPFPHSGIEGWSPKEFTNEFGVPGEVDENGTIKAVSV
                     VPRPYQEPHPPVFQPFSISESTIRYTAEKGIVPYLLLSYPEEFQRLCRVYQEVSAENG
                     YDYELGQHLGALRSIHFGDDRASARKLLEETHMKTWNLWFGKFGYWELFRLPEDEAKW
                     PLGKELLPEAEWRADRMEAHKYAFLGKPDEIKKDFEDLAKIHADGNLEYFSWYFDQGM
                     MPLEEARRQIEMFGEHIIPEFGD"
     gene            complement(1531..2778)
                     /locus_tag="VFB52_02130"
     CDS             complement(1531..2778)
                     /locus_tag="VFB52_02130"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZO05156.1"
                     /translation="MDDALAAIAAGRPVIVVDDQDRENEGDLIIAADAVSADDVAFFL
                     RHTSGVLCVALSGERLDALELPLMVPADNTEALGTAFTVTVDAREGTTTGISAADRAL
                     TIRALADPATGPADFNRPGHVFPLRAREGGVLKRAGHTEAAVDLARLAGRAPAGLLCE
                     IVTESREGMAGAEEIEALARAHSIPVIAVADLIRHRRQREKLVRRVSTAEMPTEHGLF
                     TAHVYEAILSGDQHFALVRGDLRNGDGAPPLVRVHSECLTGDVFGSRRCDCGEQLDSA
                     LERIAAEGRGAVVYLRGHEGRGIGLGHKIAAYALQERGLDTVEANLDLGLPVDSREYG
                     IGAQILSDLGVSAMRLLTNNPDKRGGLEGYGLEVAERLPLATRPTPENLTYLRTKRER
                     MGHLLGDLPGEAALPPTRKDCPK"
BASE COUNT          403 a          976 c         1058 g          503 t
ORIGIN      
        1 cgccgcctgc gccggggtca ccttgcgcgc ggtcgcgacc tcgctgggga tcgcggtcga
       61 gtccggcagc gtgcgcgccg agggcgacct cgacttccgc ggcacgctcg cggtcgatcg
      121 cgaggcgccg gtcggcttcc gcgcgatccg tctccatatc gaggtcgaga gcgccgccag
      181 cgaggaggag ctggcgacgc tcttgaagct gaccgagcgc tactgcgtcg tcctgcagac
      241 gatcgccggt gcgcccgatc tctcggcctc gattagctct gcctgactag tccccgaact
      301 ccgggatgat gtgctcgccg aacatttcga tctgccggcg cgcctcctcc aggggcatca
      361 tgccctggtc gaagtaccag ctgaagtact cgaggttgcc gtcggcgtgg atcttcgcca
      421 ggtcctcgaa gtccttcttg atttcgtccg gcttgccgag gaaggcgtac ttgtgcgctt
      481 ccatgcggtc ggccctccac tccgcttccg gcagcagctc tttgccgagc ggccacttcg
      541 cctcgtcctc gggcaggcgg aagagctccc agtagccgaa cttgccgaac cagaggttcc
      601 acgtcttcat gtgggtttct tcgagcagct tgcgcgccga ggcacggtcg tcgccgaagt
      661 ggatcgagcg cagggcgccg aggtgctggc cgagctcgta gtcgtagccg ttttcagccg
      721 agacctcttg gtagacacgg cagaggcgct ggaactcttc cgggtaggag agcagcaggt
      781 agggcacgat ccctttttcg gcggtgtagc gaatcgtcga ctcgctgatc gagaagggct
      841 ggaagaccgg cgggtggggc tcctggtagg ggcgcgggac caccgagacg gctttgatcg
      901 tgccgttctc gtcgacctcg ccggggacgc cgaactcgtt ggtgaactct ttcgggctcc
      961 agccctcgat ccccgagtgc gggaacggga ctttgtagtg ctcgccgtcg tgggtgagcg
     1021 cgtcctcggt ccaggccttg tagacgagct cgacgacctc ctcgtagatt tcccggttct
     1081 tcaggtcttt cgcggagccg tcggagagcg tgccgccgac gtggtactgc tggccgagga
     1141 tcttcaccca gcggtcctgg tagccgcggg cgaagccggc gtagtagcgg ccctggcaga
     1201 gctggtcgag gatcgcggtc tcctcggcga ggcggatcgg gtcccaggtg gtggcgccga
     1261 gaccgagggt gaggaatttg attttctcgg tgcgcgcggc gaggtcggcg aagagcatca
     1321 gcggcgcgac cgagacctcc cagccctccg agtgcagatg gtgttcggtc agcccgatcg
     1381 agtcgaagcc gacttcctcg gcgagcttgg tcaggtcgcg aacttcggtg agcatctcct
     1441 ggtagtgctc ggtgttgcgt ccgatcggcc gcagctcgcg ccgggtctcg tccgacgcgg
     1501 gaatcgtcgg cagcaggaac aggttgaact tcacttggga cagtcctttc tggtgggggg
     1561 cagcgcggcc tcgcccggca gatcgccgag caggtggccc atgcgctctc tcttcgtgcg
     1621 caggtaggtg aggttctccg gggtcggccg ggtcgccagc ggcagccgct cggccacctc
     1681 cagcccgtag ccctcgaggc cgccccgctt gtcggggttg ttggtcagaa ggcgcatcgc
     1741 cgagaccccg aggtcggaga ggatctgggc gccgatcccg tactcgcgcg agtcgaccgg
     1801 gaggccgagg tcgaggttgg cctcgaccgt gtcgaggccg cgctcctgca gcgcgtaggc
     1861 agcgatcttg tggccgagcc cgatcccgcg gccctcgtgg ccgcgcaggt agacgacagc
     1921 gccgcggccc tcggcggcga tccgctccag cgccgagtcg agctgctcgc cgcagtcgca
     1981 gcggcgcgag ccgaagacgt cgccggtcag gcactcgctg tggacgcgga ccaggggcgg
     2041 cgccccgtcg ccgttgcgaa ggtcgccgcg gaccagggcg aagtgctggt cgccgctgag
     2101 gatcgcctcg tagacgtggg ctgtgaagag accgtgctcg gtcggcatct cggcggtcga
     2161 gacccggcgg accagcttct cgcgctggcg ccggtggcgg atcaggtcgg cgaccgcgat
     2221 caccgggatc gagtgcgcgc gggcgagcgc ctcgatctcc tccgcccccg ccatgccctc
     2281 gcgggactcg gtgacgatct cgcagagcag cccggccggg gcgcggccgg cgagccgggc
     2341 gaggtcgacg gcggcctcgg tgtggccggc gcgcttcagc acgccgccct cgcgggcacg
     2401 cagcgggaag acgtggcccg ggcggttgaa gtcagcgggg ccggtcgccg gatcggcgag
     2461 cgcgcgaatc gtcagcgccc ggtcggcggc cgagatcccg gtggtcgtgc cctcgcgggc
     2521 gtcgaccgtg accgtgaacg cggtcccgag tgcctcggtg ttgtcggcgg gaaccatcag
     2581 cggcagctcc agcgcgtcga gccgctcgcc gctcagggcg acgcaaagca ccccggaggt
     2641 gtggcgcagg aagaaggcga cgtcgtccgc gctcaccgcg tccgcggcga tgatcaggtc
     2701 gccctcgttc tcgcggtcct ggtcgtcgac cacgatcacc ggacggcccg cggcaatcgc
     2761 cgccagggcg tcgtccacag tcgttccctc ctccgttgtc acgctgcgtg acaccatcca
     2821 gccgaactat acgcatgttt tgatttcgaa gtcaaggctg cccgaatttc tcgcgccagg
     2881 acagcagtcg ctccagccgc ggatcggcct ccgccggggc cagcgagagc cggtcgcggg
//
LOCUS       DASRIG010000081         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2381427, whole genome shotgun sequence.
ACCESSION   DASRIG010000081 DASRIG010000000
VERSION     DASRIG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381427"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="nadA"
                     /locus_tag="VFB52_02135"
     CDS             <1..603
                     /gene="nadA"
                     /locus_tag="VFB52_02135"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZO05157.1"
                     /translation="CTSSNAVQVVEHIWAEHGPETEILFGPDMWLGAFVERETGLPDD
                     PERRARFHIWDGECHVHAGTRPEDIAETRAANPEAEFLIHPECGCSTQAMEYVASGDI
                     EPEGVHMLSTSGMLKHVEANPDGSFIVATENGMLYPLQQAAPQANLIEANRMAFCKYM
                     KMITLPKLRDSLRDEKFEVSVAAAIAERAKVPIERMIAIG"
     gene            complement(600..1235)
                     /locus_tag="VFB52_02140"
     CDS             complement(600..1235)
                     /locus_tag="VFB52_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HZO05158.1"
                     /translation="MEELPGKRIANAAHEAGPWQIQAIVPDFTLEDAWALPVSGSGDD
                     FPAMVELLAASDPNQAESLPTRLLWRLRDRLGAWFDLGRISSPDEGSAGPPIPGTDET
                     SLAGRLPAHLRGTAADVKFASLPFVPLYLTPDEFAAEIGNQTVHGVIHLAWAERGEDL
                     YQCEMAVYVKPRGRFGQAYMAAIKPFRHLIVYPALMRQSERTWLAARRAGD"
     gene            1300..1860
                     /locus_tag="VFB52_02145"
     CDS             1300..1860
                     /locus_tag="VFB52_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05159.1"
                     /translation="MAKLRTPREAWVEAGLRALAAGGPDAVRIEALAAGLGVSKGGFY
                     WHFKDRAALLEEMLDSWEQVGTDAVIERVEAEPRAPQERLRLLFELAPAAAADFTVEL
                     AIRDWSRRDDAVAARLARIDARRLAFVQGLFGEFCASERDAEARSMLAYSLLVGSYFI
                     VAAQDGRSRGEVVQRAFDRLLDEPWS"
     gene            1851..2222
                     /locus_tag="VFB52_02150"
     CDS             1851..2222
                     /locus_tag="VFB52_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZO05160.1"
                     /translation="MELTRPLLVFDGDCFFCTRCARLLGRIGPRAEIVAWQHADLDRL
                     PITEQQASEAVQWVGVDGTVRSGHEAIAAVLASAGPLWRLAGRAMLLPGISWLAALTY
                     GLIAANRSRISRVFRKLSGTT"
     gene            2283..2624
                     /locus_tag="VFB52_02155"
     CDS             2283..2624
                     /locus_tag="VFB52_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05161.1"
                     /translation="MIDDPDTHRVLVVAGEAADYPGLIAGLQVDADPELTSFTLLVPG
                     WPETVGRAERAQARMRAAGLEVEETIVGDPDPFVAVGDVLHVRRFDEIIIADSRKMTR
                     HASADLYTSVS"
     gene            2720..3574
                     /gene="ftsE"
                     /locus_tag="VFB52_02160"
     CDS             2720..3574
                     /gene="ftsE"
                     /locus_tag="VFB52_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZO05162.1"
                     /translation="MARSRKRNQNEDQPQRSRPRRAPIIELNEVTKVYPGGHMALDRV
                     SLSIGRGEFVFLVGPTGCGKSTLIKTLIRELDTTEGSVKIAGRDIGALPEKKIPQLRR
                     NIGTVFQDFKLLPNRTVYDNVAYALQVIGANRAEIREQVPATLRLVGLSTKLHSYPDQ
                     LSGGEQQRVSVARAFVNHPPLLLADEPSGNLDPVTSIGIMQLLYRINKTGTTVVVVTH
                     DREMVDNMRRRVVELYEGRVVRDEVSGGYTEESTTEFGMRMRAEMGVGVQGEGQTNGH
                     SHDHPLSN"
     gene            3574..4479
                     /gene="ftsX"
                     /locus_tag="VFB52_02165"
     CDS             3574..4479
                     /gene="ftsX"
                     /locus_tag="VFB52_02165"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZO05163.1"
                     /translation="MSRIVFFFQEAFRALRRNGAPTLAAIVTTVVTVTLLGVLIPIFQ
                     TTQAKSDEVRSELNIQVGLYDDATPAERQQLQGELEALPHVESVAYISKAEALATLRS
                     EMKDKSIVDSLNTNPLPSSFEIKADDPANLKTIETAIIPAGANGQPDPVSPIVNNIFD
                     RQQASEEIEQVTSALKIVLTVITALLIVASLMLIGNTIRLSIYTRRREVEVMRLVGAT
                     RWFIRWPFMIEGLVVGFAGGLVAILVLWLGKLTIVDPLKGGDFDFLSAQDTMSFPLLV
                     GILFVAAVLVSTIGSGITLRRFLKV"
     gene            4487..>5225
                     /locus_tag="VFB52_02170"
     CDS             4487..>5225
                     /locus_tag="VFB52_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZO05164.1"
                     /translation="MRARGALAFAGALIAVLCLGLWLGGHPSKLPEFVRDAFVNEQGG
                     LSAEAAELIEDAYYKPVNETELSNASLQGMVRELRRRNDDQFTEYFSPEALASFNEQI
                     EGKFTGIGLSVLPVKRGLRVSQVFPGSPAKEAGIEPGDTIVSVNGKSIAGENSAEATA
                     KIKGPEGTEVTVGVLSGKTKKEREETITRAEVALPNVTSNVREVDGKKVGYVSLLSFS
                     VGAHAQLAEGVEKVEEEGAEAIVLDLRG"
BASE COUNT          887 a         1779 c         1762 g          797 t
ORIGIN      
        1 tgcaccagct cgaacgcggt ccaggtggtc gaacacatct gggccgagca cggtcccgag
       61 accgagatcc tcttcggccc cgacatgtgg ctcggcgcct tcgtcgagcg cgagaccggg
      121 ctccccgacg accccgagcg ccgcgcccgc ttccacatct gggacggcga gtgccacgtg
      181 cacgccggga cccggcccga ggacatcgcc gagacccgcg ccgccaaccc cgaggccgag
      241 ttcctgatcc accccgagtg cggttgctcg acccaggcga tggagtacgt cgcatcgggc
      301 gacatcgagc ccgagggcgt gcacatgctc tcgacctcgg ggatgctgaa gcacgtcgaa
      361 gccaaccccg acggcagctt catcgtcgcc accgagaacg ggatgctcta cccgctgcag
      421 caggccgccc cgcaggcgaa cctgatcgag gccaaccgga tggccttctg caagtacatg
      481 aagatgatca ccctgccgaa gctgcgcgac agcctccgcg acgagaagtt cgaggtcagc
      541 gtggcggccg cgatcgccga gcgggccaag gtcccgatcg agcggatgat cgcgatcggc
      601 tagtcgccgg ctcggcgcgc cgccagccag gtccgttcgg actggcgcat cagcgccggg
      661 tagacgatca ggtgtcggaa cggtttgatc gccgccatgt aggcctggcc gaagcggcca
      721 cgcggtttca cgtagacggc catctcgcac tggtagaggt cctcgccccg ttcggcccag
      781 gcgaggtgga tcacgccgtg cacggtctgg ttgccgatct cggcggcgaa ctcgtccggg
      841 gtgaggtaga ggggcacgaa gggcagcgag gcgaacttca cgtccgccgc cgtcccgcgc
      901 aggtgcgcgg gcaggcggcc ggcgagcgag gtttcgtcgg tgcccggaat cggaggtccc
      961 gcgcttccct cgtcggggga ggagatccgc ccgaggtcga accaggcgcc gagccggtca
     1021 cgcaggcgcc agagcaggcg ggtcggcagc gattccgcct ggttgggatc cgaggcggcc
     1081 agcaactcga ccatcgccgg gaagtcgtcg ccgctgccgg agaccggcag tgcccaggcg
     1141 tcctcgaggg tgaagtcagg gacgatcgcc tggatctgcc acggcccggc ttcgtgtgcg
     1201 gcgttggcga ttcgcttgcc cggcagctct tccatacgca gtcgtatgga tatcggagtt
     1261 gcttgcgctt gtcaatacgg tgccgtatag attggccgcg tggcgaagct cagaaccccc
     1321 cgcgaggcct gggtcgaggc cgggctgcga gcgcttgcgg ccggcggccc cgacgcggtg
     1381 cgcatcgagg ccctggccgc cggcctcggg gtgagcaagg gcggcttcta ctggcacttc
     1441 aaggaccgtg cggcgctgct cgaggagatg ctcgacagct gggagcaggt tgggaccgac
     1501 gccgtgatcg agcgggtcga agccgagccg cgcgcaccgc aggagcggct gcggctgctc
     1561 ttcgagctgg ccccggcggc cgccgccgac ttcacggttg agctggcgat ccgcgactgg
     1621 tcgcggcgcg acgacgccgt tgccgcccgg ctggcgcgga tcgacgcgcg gcggctggcc
     1681 ttcgtgcagg gcctcttcgg cgagttctgc gcttccgagc gcgacgccga agcccgcagc
     1741 atgctggcct actcgctgct ggtcggcagc tacttcatcg tcgccgcgca ggacggcagg
     1801 tcccgtgggg aggtcgtgca gcgggcgttc gaccgcttgc tcgacgagcc atggagctga
     1861 cccgacccct gctcgtcttc gatggcgact gcttcttctg cacgcgctgc gcgcggctgc
     1921 tggggaggat cgggccgcgg gcggagatcg tcgcctggca gcacgccgac ctcgatcggc
     1981 tgccgatcac cgagcaacag gccagcgagg cggtgcagtg ggtcggggtc gacggcacgg
     2041 ttcgctccgg gcacgaggcg atcgcggcgg ttctcgccag cgccggccca ctctggagac
     2101 tcgccggtcg ggcgatgctg ctccccggga tctcctggct cgctgcgctc acctacggcc
     2161 tgatcgccgc caaccgcagt cgcatctccc gggttttcag gaaattgtca ggcaccactt
     2221 aggtcgggct tagtggcagg gaggaggctt gctctcacga accccccgtg agaggagccc
     2281 tgatgatcga cgaccccgac acccaccgcg tccttgtggt tgctggcgaa gcggccgact
     2341 accccggcct gatcgccggg ctgcaggtgg acgcggaccc cgagctgacc agcttcaccc
     2401 tgctggtccc cggctggccg gagacggtcg gtcgcgccga gcgcgcccag gcccggatgc
     2461 gcgccgccgg cctcgaagta gaggagacga tcgtcggcga ccccgacccg ttcgtcgccg
     2521 tcggcgatgt tttgcacgtt cggcgcttcg acgaaatcat catcgccgac tcgcgcaaga
     2581 tgactcgcca cgccagtgcc gacctatata cgagcgttag ctaataattc gctaagatct
     2641 cgccaacgtt cttgggcgca gaatttcgcg cccgaagggt cgcaggagtc actctgtagt
     2701 tgcagaaccc aaggcactca tggcgagatc aagaaagcgc aaccagaacg aggatcagcc
     2761 ccagcggtcg cgaccgcggc gggcgccgat catcgagctg aacgaggtca ccaaggtcta
     2821 ccccggcggg cacatggctc tcgaccgggt ctccttgtcg atcggccgtg gcgagttcgt
     2881 cttcctcgtc ggcccgaccg gctgcggcaa gtcgacgctg atcaagaccc tgatccgcga
     2941 gctcgacacc accgaaggct cggtgaagat cgccggccgc gacatcggtg ccctgcccga
     3001 gaagaagatc ccgcagctgc gccgcaacat cggcaccgtc ttccaggact tcaagctgct
     3061 ccccaaccgg acggtctacg acaacgtcgc ctacgcgctc caggtgatcg gcgccaaccg
     3121 cgccgaaatc cgcgagcagg taccggcgac cctgcgcctg gtcggcctct cgaccaagct
     3181 gcacagctac cccgaccagc tctccggtgg cgagcagcag cgcgtctccg tcgcccgcgc
     3241 cttcgtcaac cacccaccgc tgctgctggc cgacgagccg agcggaaacc tcgacccggt
     3301 cacctcgatc gggatcatgc agctgctcta ccggatcaac aagaccggta cgaccgtcgt
     3361 cgtcgtcacc catgaccgcg agatggtcga caacatgcgg cgccgcgtgg tcgagcttta
     3421 cgagggccgc gtcgtccgcg acgaagtcag cggcggctac accgaggagt cgacgaccga
     3481 attcggcatg cgcatgcggg ccgagatggg tgtcggcgtc cagggtgagg gtcagaccaa
     3541 cggccacagt cacgaccacc ccctcagcaa ctgatgtcga gaatcgtttt cttcttccag
     3601 gaggcgttcc gcgccctgcg ccgcaacggc gccccgacgc tggccgcgat cgtgaccacg
     3661 gtggtcaccg tgaccctgct cggcgtcctg atcccgatct tccagaccac ccaggccaag
     3721 agcgacgagg tccgctcgga gctgaacatc caggtcggtc tctacgacga cgcgaccccg
     3781 gccgaacgcc agcagctgca gggcgaactg gaagcgctgc cgcacgtcga gtcggttgcc
     3841 tacatctcca aggcggaagc gctggccacc ctgcgcagcg aaatgaagga caaatcgatt
     3901 gtcgactcgc tgaacacgaa cccgctgccc tcgagcttcg aaatcaaagc cgacgacccg
     3961 gccaacctga agacgatcga aacggcgatc atcccggccg gcgccaacgg ccagccggac
     4021 ccggtctcgc cgatcgtcaa caacatcttc gaccgccagc aggcctcgga agagatcgag
     4081 caggtgacca gcgcgctgaa gatcgtgctg accgtgatca ccgcgctgct cattgtcgcc
     4141 tcgctgatgc tgatcggcaa cacgatccgg ctctcgatct acacccgccg ccgcgaggtc
     4201 gaggtgatgc ggctggtcgg cgcgacccgc tggttcatcc gctggccgtt catgatcgag
     4261 ggcttggtgg tcggcttcgc cggcggtctg gtggccatac tggtcctgtg gctgggcaag
     4321 ctgacgatcg tcgacccgct gaagggcggg gacttcgact tcctctccgc gcaggacacg
     4381 atgagcttcc cgctgctggt cggcatcctc ttcgtcgccg cggtgctggt ctcgacgatc
     4441 ggctcgggca tcaccctgcg gcgcttcctc aaggtctgag gcgcgggtga gagcgcgcgg
     4501 ggcgctcgcc tttgccggcg cgctgatcgc cgtcctctgc ctcgggctct ggctcggcgg
     4561 gcacccctcg aagctgcccg agttcgtccg cgacgccttc gtcaacgaac agggcgggct
     4621 gagcgcggaa gcggcggaac tgatcgagga cgcctactac aagccggtca acgagaccga
     4681 actgagcaat gcctcgctgc agggcatggt ccgcgagctg cgccgccgta acgacgacca
     4741 gttcaccgaa tacttctccc ccgaggccct ggccagcttc aacgagcaga tcgaaggcaa
     4801 gttcaccggg atcgggctct cggtgctgcc ggtcaaacgt gggctgcggg tgagccaggt
     4861 cttcccgggc tcgccggcca aggaggccgg gatcgaaccg ggcgacacga tcgtctcggt
     4921 caacggcaaa tcgatcgccg gcgagaacag cgccgaggcg acggcgaaga tcaagggccc
     4981 ggaggggacc gaggtgaccg tcggcgtgct cagcggcaaa acgaaaaaag agcgcgaaga
     5041 gacgatcacc cgggccgagg tcgcgctgcc gaacgtgacc agcaacgtgc gcgaggtcga
     5101 tgggaagaag gtcggctacg tgagcctgct cagcttcagc gtcggcgccc acgcgcagct
     5161 cgccgaaggg gtggaaaaag tcgaggaaga aggggcggaa gcgatcgtgc tcgacttgcg
     5221 cggca
//
LOCUS       DASRIG010000082        11303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1095028, whole genome shotgun sequence.
ACCESSION   DASRIG010000082 DASRIG010000000
VERSION     DASRIG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095028"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFB52_02175"
     CDS             complement(<1..894)
                     /locus_tag="VFB52_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZO05165.1"
                     /translation="MLRSSSRLPLLALLALCVALLVPAGASAAIGPLSSFGGFGEAAG
                     QLRAPQGLAIGSESEVYVADEDNNRIDVFSAAGTFVRAFGKDVGGAGIDVCTSFCQAG
                     EFGIGTAGALYEPVDVALDGSGRVYVADPQNSRIDVFTTAGAFLFAFGKDVKLGGGNR
                     CDAGTGCQSGAFESGAGAFGELEGIGIGGGRLYAADADNNRVAVYDLEGKFQFAFGRN
                     VGGAGVHTCTSVCQAGSAEGKAAIDNPTDVAVSPDGNLYVTFNGGTSGVKIFTAAGVD
                     GVEFGDEGPGFVDGPTAVAIGP"
     gene            complement(925..2787)
                     /gene="cysC"
                     /locus_tag="VFB52_02180"
     CDS             complement(925..2787)
                     /gene="cysC"
                     /locus_tag="VFB52_02180"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZO05166.1"
                     /translation="MSSELLRIATAGSVDDGKSTLIGRLLHDTKSIFEDQLESIELTS
                     QKRGNEQTDLALLTDGLRAEREQGITIDVAHRYFATPKRKFVIADTPGHIQYTRNMVT
                     GASTANLALLLVDARKGVLEQTRRHALLSSLLRVPHLVLCVNKMDLVDYDQAIYDEIR
                     EEFKSFAAKLEITDLGFVPISALEGDNVVNRSLNMPWFDGQPLLGHLEDVHIASDRNL
                     VDNRFPVQYVIRPQTEEHRDYRGFAGSVVGGIFKQGDEVMVLPSRRTSRIAQIDTPDG
                     AVAQAFPPMAVTMLLEDELDVSRGDMICRPTNQPSANRRFEAMICWFDETTTLRTGAP
                     YQLKHTSKWVAAEVEDMRYQLNVDTLHRDLEADTLEVNDIGRVSIHTTSPLFFDEYRL
                     NRNTGSFILVDPATDLTVAAGMIIGAGGEASTGTHSIVAENITFHPSKLSREDRWGAL
                     DTAGATIWMTGLSGSGKSTIATAVEHSLVSSGRSAYMLDGDNLRHGLNSNLGFSEEDR
                     AENVRRTGEVAKILAEAGTVVVVSLVSPYAAERDKVRALHEEAGLPFYEVFVDTPLEI
                     CEERDPKGLYAKARAGEITNLTGVGAPYEAPERAELVPDADLETAVRQVLGLLG"
     gene            complement(2787..3716)
                     /gene="cysD"
                     /locus_tag="VFB52_02185"
     CDS             complement(2787..3716)
                     /gene="cysD"
                     /locus_tag="VFB52_02185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZO05167.1"
                     /translation="MEEVTKVRSAPVELTDLDTLEAESIHIIREVVAEFENPVMLFSG
                     GKDSVLMLHLARKAFAPAPVPFPIMHVDTGHNFPEVIEFRDRVAAEHGLRLEVASLQE
                     LIDAGRIKVGHELESRNRHQTVALLEGIQKGGFDAVFGGGRRDEEKARAKERVFSFRD
                     KFGQWDPKNQRPELWNLYNGRHRKGEHIRVFPISDWTELDVWRYLAQEKVDLPQIYFA
                     HEREVVEVEGLLMAVNEFFPLPEGMTPERRMVRYRTVGDATATGAVASEATDYELVIE
                     ETAATRITERGATRGDDRFSEAAMEDRKKEGYF"
     gene            complement(3753..4472)
                     /locus_tag="VFB52_02190"
     CDS             complement(3753..4472)
                     /locus_tag="VFB52_02190"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZO05168.1"
                     /translation="MKPPPITLSGTVPTVTDIDHEKVAAELEDRSAQEAFEWMFESFG
                     SDHYIACSFQKTSTVTAHLASTVNPEARFFYLDTEVLFEESYETRDRLAEALGIEFDR
                     YHNLTLAEQAEQYGDELWKHDPDACCGLRKVDPMRRALSSVECWVAGVRRGDSSSRAK
                     TPKFSWDKKFNLWKLNPLADWTERDVWTYISEHQLPYNPLHDQGYPSIGCTHCTQPVG
                     PGGSSRDGRWVGMGKTECGIN"
     gene            complement(4487..6328)
                     /locus_tag="VFB52_02195"
     CDS             complement(4487..6328)
                     /locus_tag="VFB52_02195"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZO05169.1"
                     /translation="MEVIVAENKEQRGKGRVGTVENPETYVNVPGHIIPIIEREFDDF
                     DNEAESFLAGQIEEPEFIGFRLKQGVYGQRQPDVQMVRVKLPFGGVTPDQMDTFADVV
                     EKYAPLNKGHITTRQNIQIHHVPLRDMEALIREISDAGLSSREGCGNTVRNVTGDPWA
                     GVAEDEIFDPTGHAIAYVRYFVRHPTTQLMPRKIKTAFTGSDEDRAITGIHDIAFISR
                     EKDGVKGFEIRVGGGTSIMARVAPTLYDFVEADNGDYLKVSEAVFRIFDRQDWLRVNR
                     ARARIKVLVDKIGMDAFRELVDEELEGDWVGEREFGPELVERLYFEDDEEGNAPKAPP
                     EPASPNGDRTEFERFVAANVQAQRQEGFSTVEVKIERGDLTPEQFRGLGQIMRDYSGG
                     YARSTVQQNLVLRWVRDEALYNVWTELEKLGLAEVGPRQVTDVVSCPGTDSCKLGITS
                     SMGLNRAIKQRVTEMQIDDELTKRIHVKMSGCPNGCSQHHIANIGFYGASLKVGDRQM
                     PAYIPHVGGNFEGGEVIFGKRLKSRLPAKRVPEAVERWIRLYEKDRNEGEEFNPFAER
                     VGAEPFEAEIKDLTLPAEFSLETMQQFIDWNRSSPYKVERGEGECAI"
     gene            complement(6472..6858)
                     /locus_tag="VFB52_02200"
     CDS             complement(6472..6858)
                     /locus_tag="VFB52_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZO05170.1"
                     /translation="MSEAGEPREVSREEAQKLVAEGAQLVDVRADHEWEVGRIAGATH
                     LPLAELAERAGEIDKERPVVVYCRGGNRSSMATAALSEAGYDAVKLTEGITGWAEDGL
                     PLEPEGGYVAESGEAAAVLQARKRAG"
     gene            complement(6858..7067)
                     /locus_tag="VFB52_02205"
     CDS             complement(6858..7067)
                     /locus_tag="VFB52_02205"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZO05171.1"
                     /translation="MAVVKIIELVGSSKTSTDDAAKQALDQAKGSLRNIRAVDIVSTG
                     IRGENLDEYRAHVRVAFLIEGTEVD"
     gene            complement(7055..7258)
                     /locus_tag="VFB52_02210"
     CDS             complement(7055..7258)
                     /locus_tag="VFB52_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05172.1"
                     /translation="MGQKTATGDEAAAIAAAVARFEAETAAVTADDSPAAVSPWQRAA
                     LLEGVGAKEAVQESKGKGSTWLS"
     gene            complement(7324..9057)
                     /locus_tag="VFB52_02215"
     CDS             complement(7324..9057)
                     /locus_tag="VFB52_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZO05173.1"
                     /translation="MNRSIRGALLALALVCAGFAMLGAAAPEPEQDEPARCHGRIAKI
                     VGTDGDDVLHGTPSRDVIWGGNGDDEIYGSLGNDLICGGPGADLIHGGRGNDEVDGSA
                     GDYDRVIGDLGDDKVMGGGGDHDEAAGSLGIDIVNGGAGAFDLVRGDYGWDRMSGGAG
                     KGDIASFATKVAGGKGTGVWVSLQEHIARGDGHDKLFGFEDIEGSAFRDTLIGSKSNN
                     HVDGGLGDDKISGAGGIDTIDGGQGSDRCTGAKGRTVSCGRERAPKASAYIQLNPLAR
                     GGAGLQIVGGSGNDDFVVLFDEKTETYGVSAKKPLAIGSGCERQTPAQVACHAEGPGL
                     WLMADLGPGNDSLRVEGSLKAVGTTRLAGGFGNDVIHGGPEDDLIEAGPGADKLYGGA
                     GADGLVGGLPGPTYLYGGPNGDLLAAGGGCAGGGIFGGPGRDDSSFAETQAHPGLLII
                     SFPQHKAWVDEGPCDPVYLAPDLEDMEGSFDNDVLIGNGQKNAMLGQPGHDRFFGNGG
                     DDVIDARDGVRDFSIQCGRGNPPQRLPKGKGFSKPTGVAAGRALMDSFDPASFNCAIT
                     KYGTPVPGLHG"
     gene            complement(9089..10456)
                     /locus_tag="VFB52_02220"
     CDS             complement(9089..10456)
                     /locus_tag="VFB52_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZO05174.1"
                     /translation="MSTYAVVNPATGEKLKEYPEISDADLRAAIDRADRAYKSWSGSS
                     TIAERAALVRKVGELHSERREVLAEIIVREMGKPVEQALGEVDFAAAIYGFYADNAES
                     IMADEPIELLEGDGTAVVRRSAFGVLLGIMPWNFPYYQVARFAGPNLVIGNPILLKHA
                     PQCPESAEAMQQIFDDAGAPEGAYENVYATNEQIEWVIGDPRVHGVSVTGSERAGAAV
                     AEIAGRNLKKVVLELGGSDPFILLGTNDVDAAATQAAEARLDNTGQSCNAAKRFIVVD
                     ELYDEFVAKLTEKMSGAKSGDPTAEDTEVGPLSSATAADRLEDQVKRAIENGAEVVTG
                     GKREGNYFEPTVLTGIAPGNEASQEEFFGPVAQVYKVADEDEAVALANQTPFGLGSYV
                     MSTDKEQAERVADKIEAGMVFVNLVLADGVELPFGGFKRSGFGRELGRYGADEFVNKK
                     LIRSA"
     gene            complement(10457..>11303)
                     /locus_tag="VFB52_02225"
     CDS             complement(10457..>11303)
                     /locus_tag="VFB52_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZO05175.1"
                     /translation="PEQTRGLCESGATAIAYETVEDARGRLPLLAPMSEIAGKIATQA
                     GAFMLEKPLGGRGILLGGVPGVAAANVMVIGGGVVGMNAAFIAIGMEADVFVFDRSID
                     RLRELEVAFGGRCSTVYSTTLAVEEMLPRADLVIGAVLVHGARAPYVIRRDQLALMKP
                     GAVLVDVAIDQGGCFETSRPTTHRDPTFEVDGITHYCVANMPGAVPITSTHALTNATL
                     PYAIALADHGVAEAIRRDPGLRPGINVAGGRVTHPAVAEGVGMDYVPVEEALGLDQVT
                     PQGRS"
BASE COUNT         1572 a         3812 c         3952 g         1967 t
ORIGIN      
        1 cggtccgatc gccaccgcgg tcggaccgtc gacgaaaccg ggaccttcgt cgccgaactc
       61 caccccgtcg acgccggcag cggtgaagat cttgaccccg gaggtgcccc cgttgaacgt
      121 gacgtaaaga ttcccgtcgg gcgagaccgc gacgtcggtt gggttgtcga tcgcggcttt
      181 cccttctgcc gagcccgcct ggcacacgct cgtgcaggtg tgcaccccgg cgccgccaac
      241 gttcctgccg aaggcgaact ggaatttccc ctcgaggtcg tagacggcaa cgcggttgtt
      301 gtcggcatcg gcggcgtaga ggcggccgcc gccgatgccg atcccttcca gttcgccgaa
      361 ggctccggcc ccagattcga aggcgccgga ctggcagccg gtccccgcgt cgcaccggtt
      421 gccgccgccc agtttcacgt ctttgccgaa agcgaagagg aacgcgccgg cggtcgtgaa
      481 gacgtcgatg cggctgttct gggggtcggc gacataaacc cgcccgctcc cgtccagcgc
      541 cacgtcgacc ggttcgtaca aagccccggc cgtgccgatt ccgaattcac ccgcctggca
      601 gaagctcgtg cagacgtcga tcccggcgcc cccgacgtcc ttgccgaagg cgcgcacgaa
      661 ggtgccggcg gccgagaaga cgtcgatccg gttgttgtct tcgtcggcga cgtagacctc
      721 agattcggag ccgatcgcca gaccctgcgg cgcccgcagc tggcccgcgg cttcgccgaa
      781 gccgccgaag ctggagaggg ggccgatcgc cgccgaggcg ccggcgggga cgagcagcgc
      841 cacgcagagc gcgagcaggg cgagcagcgg gagccgggac gaagagcgaa gcatggccga
      901 gccgcgaaga ctcgcagccg ggagctagcc cagcaacccc aaaacttggc gaaccgcggt
      961 ctccaggtcg gcgtccggga ccagctccgc gcgctcgggc gcctcgtagg gggcgccgac
     1021 gccggtcagg ttggtgatct cgcccgctcg ggccttcgcg tagaggccct tcgggtcgcg
     1081 ttcttcgcag atctccagcg gcgtgtcgac gaagacctcg tagaacggca gccccgcctc
     1141 ttcgtgcagg gcgcggacct tgtcgcgctc ggccgcgtag gggctgacca gggagacgac
     1201 gacaacggtg ccggcttcgg cgaggatctt cgccacctcg ccggtgcggc gcacgttctc
     1261 ggcccggtcc tcctcgctga acccgaggtt ggagttgagc ccgtggcgca ggttgtcgcc
     1321 gtcgagcatg taggcggagc ggccgctgga gacaaggctg tgctcgaccg cggtcgcgat
     1381 cgtcgacttg cccgagcccg agaggccggt catccagatc gtcgcgccgg cggtgtcgag
     1441 cgctccccag cggtcctcgc gcgagagctt gctcgggtgg aaggtgatgt tctcggcgac
     1501 gatcgagtgc gtgccggtgc tcgcctcgcc gccggcgccg atgatcatcc cggcggcgac
     1561 ggtgaggtcg gtggcggggt cgacgaggat gaagctgccg gtgttgcggt tcagccggta
     1621 ctcgtcgaag aagagcggcg acgtcgtgtg gatcgagacc cggccgatgt cgttgacctc
     1681 gagcgtgtcc gcctcgagat cgcggtgcag ggtgtcgacg ttgagctggt agcgcatgtc
     1741 ctcgacctcg gcggcgaccc atttcgaggt gtgcttgagc tggtacgggg cgccggtgcg
     1801 cagcgtcgtg gtctcgtcga accagcagat catcgcctcg aagcggcggt tcgcgctcgg
     1861 ctggttggtc gggcggcaga tcatgtcgcc gcgcgagacg tcgagctcgt cctcgagcag
     1921 catcgtcacc gccatcggcg ggaaggcctg cgcgacagcg ccgtccggcg tgtcgatctg
     1981 ggcgatccgc gaggtgcgcc gcgagggcag caccatcact tcgtcgccct gcttgaagat
     2041 gccgccgacg accgagccgg cgaagccgcg gtagtcgcgg tgctcctcgg tctgcggccg
     2101 gatcacgtac tggaccggga agcggttgtc gaccaggttg cggtcggagg cgatatggac
     2161 gtcctcgagg tggccgagca gcggctgccc gtcgaaccag ggcatgttga gcgagcggtt
     2221 gacgacgttg tcgccctcca gggccgagat cgggacgaat ccgaggtcgg tgatctcgag
     2281 cttcgcggcg aaggatttga actcctcgcg gatctcgtcg tagatcgcct ggtcgtagtc
     2341 gaccaggtcc attttgttca cgcagaggac caggtggggg acgcgcagga gcgaggagag
     2401 cagcgcgtgg cggcgggtct gttcgagcac gcctttgcgc gcgtcgacca ggagcagcgc
     2461 caggttcgcg gtcgaggcgc cggtgaccat gttgcgcgtg tactggatgt gcccgggcgt
     2521 gtcggcgatc acgaacttgc gcttcggcgt cgcgaagtag cggtgggcga cgtcgatcgt
     2581 gatcccctgc tcgcgctcgg cccgcaggcc gtcggtgagc agcgccaggt cggtctgctc
     2641 gttgccgcgc ttctgcgagg tcagctcgat cgactcgagc tggtcctcga agatcgattt
     2701 ggtgtcgtgc aggagacgcc cgatcagggt cgacttgccg tcgtcgaccg agccggcggt
     2761 ggcgatgcgc aggagctccg aggacactag aaatagcctt ctttcttgcg gtcctccatg
     2821 gccgcttccg agaagcggtc gtcgccacgg gtcgccccgc gctcggtgat ccgggtcgcg
     2881 gcggtctcct cgatcacaag ctcgtagtcg gtcgcttcgg aggcgacggc gccggtcgcg
     2941 gtcgcgtcgc cgacggtccg gtagcggacc atccgtcgct cgggggtcat gccctcgggc
     3001 agagggaaga actcgttgac cgccatcagc aggccctcga cctcgaccac ctcgcgctcg
     3061 tgggcgaagt agatctgggg caggtcgacc ttctcctggg cgaggtagcg ccagacgtcg
     3121 agctcggtcc agtcggagat cgggaagacg cggatgtgct cgcccttgcg gtggcggccg
     3181 ttgtagaggt tccagagctc gggccgctgg ttcttcgggt cccactggcc gaatttgtcg
     3241 cggaaggaga agacccgctc cttcgcccgc gccttctcct cgtcgcggcg gccgccgccg
     3301 aagaccgcgt cgaagccgcc tttctggatc ccctcgagca gggcgacggt ctggtggcgg
     3361 ttgcggcttt cgagttcgtg gccgaccttg atccggccgg catcgatcag ctcctgcagc
     3421 gaagcgacct cgagccgcag cccgtgctcg gcggcgacgc ggtcgcggaa ctcgatcacc
     3481 tcggggaagt tgtgcccggt gtccacgtgc atgatcggga acggaaccgg ggcgggcgcg
     3541 aacgccttgc gcgccaggtg cagcatcagc accgagtcct tgccgccgga gaacagcatc
     3601 accgggttct cgaactcggc gacgacctcg cggatgatgt ggatcgactc ggcttcgagc
     3661 gtgtccaggt cggttagctc gaccggcgcg gaccgcacct tcgtgacctc ctccatcacc
     3721 gcatcgacct caggaccggc gccggagtgg cgctagttga tcccgcactc ggtcttgccc
     3781 atgccaaccc agcgcccgtc gcgcgaggag ccgccaggac cgaccggctg cgtgcagtgc
     3841 gtgcacccga tcgacgggta gccctggtcg tgcagcgggt tgtacgggag ttggtgctca
     3901 ctgatgtagg tccagacgtc gcgctcggtc cagtcggcga gcgggttcag cttccagagg
     3961 ttgaatttct tgtcccagga gaacttggga gtcttggcgc gtgagctgga atcgccacgg
     4021 cggacgccag cgacccagca ttctacggaa gagagcgccc tccgcatcgg gtcgaccttg
     4081 cgcaaaccgc agcaggcgtc ggggtcgtgc ttccagagct cgtcgccgta ctgctcggcc
     4141 tgctcggcga gggtcaggtt gtggtaccgg tcgaactcga tcccgagcgc ctcggcgagc
     4201 cggtcgcggg tctcgtagct ctcctcgaag aggacctcgg tgtcgaggta aaagaagcgc
     4261 gcctccggat tgaccgtgct cgccaggtgg gcggtcaccg tgctcgtttt ctgaaacgag
     4321 caggcgatgt agtggtcgga gccgaagctc tcgaacatcc actcgaaggc ttcctgcgcg
     4381 gagcggtcct cgagttcggc ggcaaccttc tcgtggtcga tgtcggtgac tgtcggcacc
     4441 gtcccggaga gagtgatcgg cggtggcttc atagtccccg cgcgcgttag atcgcgcact
     4501 ccccctcgcc gcgctcgact ttgtacgggc tggagcgatt ccagtcgatg aactgctgca
     4561 tcgtctcgag gctgaactcg gccggcagcg tcaggtcctt gatctccgct tcgaacggct
     4621 cggcaccgac ccgctcggcg aatgggttga actcctcgcc ctcgttgcgg tccttctcgt
     4681 agagcctgat ccagcgctcg accgcctccg ggacccgctt ggccgggagc cgcgatttga
     4741 gccgcttgcc gaagatcacc tcgccgccct cgaagttgcc tccgacgtgc gggatgtagg
     4801 cgggcatctg gcggtcgccg actttcagcg aggcgccata gaagccgatg ttggcgatgt
     4861 ggtgctggct gcacccgttc gggcagccgc tcattttcac gtggatccgc ttggtcagct
     4921 cgtcgtcgat ctgcatctcg gtgacccgct gcttgatcgc ccggttgagg cccatcgagc
     4981 tggtgatccc gagtttgcag ctgtcggtgc cggggcagct gaccacgtcg gtgacctgcc
     5041 gcgggccgac ctcggcgagg ccgagcttct cgagctcggt ccagacgttg tagagcgcct
     5101 cgtcgcggac ccagcgcagg accaggttct gctgcacggt gctgcgggcg tagccgccgc
     5161 tgtagtcgcg catgatctgg ccgaggccgc ggaactgctc cggggtgagg tcgccgcgct
     5221 cgatcttcac ctcgacggtg gagaagccct cctggcgctg cgcctgcacg ttggcggcga
     5281 caaagcgctc gaactcggtg cggtcgccgt tcggcgaagc cggctcgggc ggcgccttcg
     5341 gggcgttgcc ctcctcgtcg tcctcgaagt acaggcgctc gaccagctcg gggccgaatt
     5401 cgcgctcgcc gacccagtcg ccttcgagct cctcgtcgac cagctcgcgg aaggcgtcca
     5461 tgccgatctt gtcgaccagc accttgatcc gggcgcgggc gcggttgacg cgcagccagt
     5521 cctggcggtc gaagatgcgg aagacggcct cggagacctt caggtagtcg ccgttgtcgg
     5581 cctcgacgaa gtcgtagagc gtcggcgcga cgcgggccat gatcgaggtg ccgccgccga
     5641 cccggatctc gaagcccttg accccgtcct tttcgcggct gatgaaggcg atgtcgtgga
     5701 tcccggtgat cgcccggtcc tcgtcggagc cggtgaaggc ggtcttgatc ttgcgcggca
     5761 tcagctgcgt cgtcgggtga cggacgaagt agcgcacgta ggcgatcgcg tggccggtcg
     5821 ggtcgaagat ctcgtcctcg gcgacgcccg cccaggggtc gccggtgacg ttgcgcaccg
     5881 tgttgccgca gccctcgcgc gaggagaggc cggcgtcgga gatttcgcgg atcagcgcct
     5941 ccatgtcacg cagcggcacg tggtggatct ggatgttctg ccgcgtggtg atgtggccct
     6001 tgttgagggg cgcgtacttc tcgaccacgt cggcgaaggt gtccatctgg tccggcgtga
     6061 ccccgccgaa cggaagcttt acccggacca tctgcacgtc gggctggcgc tgcccgtaga
     6121 cgccctgttt gagacggaag ccgatgaact ccggctcctc gatctggccg gcgaggaacg
     6181 actcggcttc gttgtcgaag tcgtcgaact cgcgctcgat gatcgggatg atgtgacccg
     6241 ggacgttgac gtaggtctcc gggttctcga cggtgccgac gcggcccttg ccgcgctgct
     6301 ccttgttctc agcgactatt acctccatgc ggaggaaaat agcaaaacct gaccgggatt
     6361 agggtattta gctattagca ggcaatttgc ggcccggttt caggccccga gccgctttcg
     6421 gggttttacg aacaggggtt tttgagcgga ccagcgaccc gctcaacccc ctcagccggc
     6481 ccgcttgcgc gcctgcagga ccgctgccgc ttcgccggac tcggcgacgt agccgccctc
     6541 cggctccaac ggcaggccat cctcggccca gccggtgatc ccctcggtca gcttgaccgc
     6601 gtcgtagccg gcctcgctga gcgccgccgt cgccatcgag gagcggttgc cgccgcggca
     6661 gtagacgacg accggccgct ccttgtcgat ctcgcccgcg cgctcggcca gctcggccag
     6721 cggcaggtgg gtggcaccgg cgatccggcc gacctcccac tcgtggtcgg cgcgcacgtc
     6781 gaccagctgc gcgccctcgg ccacgagctt ctgtgcctcc tcacgcgaaa cctcgcgcgg
     6841 ctcccccgcc tcgctcatca gtcgacctcc gttccctcga tcaggaaggc gacgcgcacg
     6901 tgcgcccggt actcgtcgag gttctcgccg cggatcccgg tcgagacgat gtcgacggcg
     6961 cggatgttgc gcagcgaccc tttcgcctgg tcgagcgcct gtttggcagc gtcgtcggtc
     7021 gaggtcttgg agctcccgac cagctcgatg attttcacga cagccatgtc gatccctttc
     7081 ctttcgactc ctggaccgcc tccttggccc ctaccccttc aagcagcgcc gcccgctgcc
     7141 aagggctgac cgccgccgga gagtcgtccg cagtgacggc cgcggtctcc gcctcgaacc
     7201 gcgcgacggc agccgcaatc gcggctgcct cgtctccggt cgcggtcttt tgtcccacgg
     7261 atctagccta gttcgtcgcg gcgcctggcg aggtgctgac cgagccggcg cacgactgcc
     7321 gccctagccg tggaggccgg ggacgggggt gccgtatttg gtgatcgcgc agttgaagga
     7381 ggctgggtcg aagctgtcca tcagggcgcg gccggctgcg acgccggtgg gtttggagaa
     7441 gcctttgcct ttcggcaggc gttgcggggg gttgccgcgg ccgcactgga tcgagaagtc
     7501 gcggacgccg tcgcgagcgt cgatcacgtc gtcgccgccg ttgccgaaga agcggtcgtg
     7561 gccgggctgg ccgagcatcg cgtttttctg cccgttgccg atcaggacgt cgttgtcgaa
     7621 cgagccctcc atgtcctcga ggtcgggcgc gaggtagacg gggtcgcagg ggccctcgtc
     7681 gacccaggct ttgtgctggg gaaaggagat gatcaggagc cccgggtgcg cctgggtctc
     7741 ggcgaaggag gagtcgtcgc ggccggggcc gccgaagatc ccgccgccgg cgcagccgcc
     7801 gccggccgcc agcaggtcgc cgttggggcc gccgtagagg taggtcgggc cgggcaggcc
     7861 gccaaccagg ccgtcggcgc cggcgccgcc gtagagtttg tcggcgccgg ggccggcctc
     7921 gatcaggtcg tcctcgggac cgccgtggat cacgtcgttg ccgaagccac cggcgagccg
     7981 cgtggtgccg accgctttca gtgagccttc gacccgcagg ctgtcgttgc cgggcccgag
     8041 gtcggccatc agccagaggc cggggccttc ggcgtggcag gcaacctggg ccggagtctg
     8101 gcgctcgcag cccgagccga tcgcgagcgg ctttttcgcg ctgacaccgt aggtctcggt
     8161 tttctcgtcg aagaggacga cgaagtcgtc gttgccggag ccgccgacga tctgcaggcc
     8221 ggcgccgccg cgggcgagcg ggttgagctg gatgtaggcc gaggctttcg gcgcccgttc
     8281 gcggccgcag gagacggtcc gccctttggc gcccgtgcaa cggtcgctgc cctggccacc
     8341 gtcgattgtg tcgatgccgc cggcgccgct gatcttgtcg tcgccgaggc ccccgtcgac
     8401 gtggttgttg ctcttgctgc cgatcagcgt gtcgcggaag gccgagcctt cgatgtcctc
     8461 gaacccgaac agcttgtcgt ggccgtcgcc gcgcgcgatg tgctcctgca gcgagaccca
     8521 gacgccggtg cctttgccgc cggcgacctt ggtcgcgaag gaggcgatgt cgcccttgcc
     8581 ggcgccgccg gacatccggt cccagccgta gtcgccgcga accaggtcga aggcgccggc
     8641 accgccgttg acgatgtcga tcccgaggct gccggcggcc tcgtcgtggt cacctccacc
     8701 acccatgacc ttgtcgtcgc cgaggtcgcc gatcacccgg tcgtagtcgc cggcgctgcc
     8761 gtcgacctcg tcgttgccgc ggccgccgtg gatcaggtcg gcgcccgggc cgccgcagat
     8821 caggtcgttg ccgaggctgc cgtagatctc gtcgtcgccg ttgccgcccc agatcacgtc
     8881 gcggctcggc gtcccgtgca ggacgtcgtc gccgtcggtg ccgacgatct tcgcgatccg
     8941 cccgtggcag cgcgccggct cgtcctgctc gggctccggt gccgccgctc caagcatggc
     9001 gaagcccgcg cagaccagcg ccagcgcgag caatgccccc ctgatagagc ggttcaccaa
     9061 gatcggatcg tagtaacccg agttatgcct aagcggagcg gatcagcttt ttgttcacga
     9121 actcgtcggc gccgtagcgg cccagctcgc ggccgaaccc ggagcgcttg aagccaccga
     9181 acggcagctc gaccccgtcg gcgaggacca ggttgacgaa aaccatcccg gcttcgatct
     9241 tgtcggcgac ccgctcggcc tgctccttgt cggtgctcat cacataggag ccgagcccga
     9301 acggcgtctg gttcgcgagc gcgaccgcct cgtcctcgtc ggcaaccttg tagacctggg
     9361 cgaccgggcc gaagaactct tcctgggaag cctcgttgcc gggcgcgatc ccggtcagca
     9421 ccgtcggttc gaagtagttg ccctcgcgct tgccgccggt cacgacctcg gcgccgttct
     9481 cgatcgcccg cttgacctgg tcctcgagcc ggtcggccgc ggtggccgag gagagcggcc
     9541 cgacttcggt gtcctccgcg gtcgggtcgc ccgacttcgc cccgctcatc ttctcggtca
     9601 gcttggcgac gaactcgtcg tagagctcgt cgacgacgat gaaccgcttg gccgcgttgc
     9661 aggactggcc ggtgttgtcg agccgcgctt ccgccgcctg cgtcgcggca gcgtcgacgt
     9721 cgttggtgcc gagcaggatg aacggatcgg agccgccgag ctcgagcacg accttcttca
     9781 ggttgcggcc agcgatttcg gcgaccgccg cgcccgcccg ctcggagccg gtgaccgaga
     9841 cgccgtgcac gcgcgggtcg ccgatcaccc actcgatctg ctcgttggtc gcgtagacgt
     9901 tctcgtaggc gccctcgggg gcgccggcgt cgtcgaagat ctgctgcatc gcctcggccg
     9961 attccgggca ctggggagcg tgcttgagca ggatcgggtt gccgatcacc aggttcggtc
    10021 cggcgaagcg ggccacctgg tagtagggga agttccaggg catgatcccg agcagcaccc
    10081 cgaaggcgct gcgccgcacg accgcggtgc cgtcgccttc gagcagctcg atcggctcgt
    10141 cggccatgat gctctccgcg ttgtccgcgt agaagccgta gatcgcggcg gcgaagtcga
    10201 cctcgccgag cgcctgctcg accggcttgc ccatctcgcg gacgatgatc tcggccagca
    10261 cctcgcggcg ctcggagtgc agctcgccga ccttgcggac gagcgcggcg cgctcggcga
    10321 tcgtggagga gcccgaccag gatttgtagg cgcggtcagc gcgatcgatc gcggcgcgca
    10381 gatcggcgtc gctgatctcc gggtactcct tgagcttctc gcccgtcgct gggttgacta
    10441 ccgcgtaggt gctcattcag ctcctccctt ggggggttac ctggtcgagg ccgagtgcct
    10501 cctcaaccgg aacgtagtcc atgcctactc cctccgcgac cgccgggtgc gtgacccgcc
    10561 cccccgcgac gttgatgccc ggccgcaggc cggggtcgcg gcggatcgcc tcggcgacgc
    10621 cgtggtcggc gagcgcgatc gcgtaaggca gggtcgcgtt ggtgagcgcg tgggtcgagg
    10681 tgatcggcac cgcgccgggc atgttggcga cgcagtagtg ggtgatcccg tcaacctcga
    10741 aggtggggtc gcgatgagtg gtcggccgcg aggtctcgaa gcagccgccc tggtcgatcg
    10801 ccacgtcgac gagaaccgcg ccgggcttca tcagcgccag ctggtcgcgg cggatcacgt
    10861 agggggcgcg ggcgccgtgc acaagcaccg cgccgatcac gaggtcggcg cgcggcagca
    10921 tctcctcgac cgcgagcgtg gtcgagtaga cggtcgagca gcgcccgccg aaggcgactt
    10981 cgagctcgcg cagacggtcg atcgagcggt cgaagacgaa cacgtcggct tccatcccga
    11041 tcgcgatgaa ggcggcgttc atcccgacca cgccgccgcc gatcaccatc acgttggcag
    11101 ccgccacccc gggcacgccg ccgagcagga tcccgcgccc gccgagcggc ttctcgagca
    11161 tgaaggcgcc ggcctgggtc gcgatcttgc cggcgatctc gctcatcggc gccagcagcg
    11221 gcagccgccc gcgcgcgtcc tcgacggtct cgtaggcgat cgccgtcgcc cccgactcgc
    11281 ataggccgcg ggtctgctcg ggg
//
LOCUS       DASRIG010000083         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_813975, whole genome shotgun sequence.
ACCESSION   DASRIG010000083 DASRIG010000000
VERSION     DASRIG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813975"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..691
                     /locus_tag="VFB52_02230"
     CDS             311..691
                     /locus_tag="VFB52_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05176.1"
                     /translation="MNEPATISGWWVFAGVLLIVAGLLNIVWGIAAIGDSKFFTDHGT
                     FILASLNTWGWIMLIVGVLEMVAAFSLFAGGEFGRWFGIFIGAVNSVGALLSIPAYPF
                     WSLAVFALSIIIIYKLAEGPEGSV"
     gene            727..1626
                     /locus_tag="VFB52_02235"
     CDS             727..1626
                     /locus_tag="VFB52_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HZO05177.1"
                     /translation="MLRSSVEAELVPRFAGLLRDIDGVRRVVQQPDESEERGCSVFVA
                     DVEPAAADRLVEAIAELGIPVDDYVLSKIDVVAPLRRQLGGDHGIEGFAWVEVMGQAR
                     ANSRPLARYLALISVAAVIAACGVITSSSILIVGAMAISPDVLPICATCVGIVGGRRR
                     LAERSAITLTLGLVLVVVVTAVLSALLNAGDFLPSGFKVEDSALSTLAHTDYSTVLVA
                     LAAGVAAMLAFETRASAAVGVAISVTTMPASAYLGVSIGAGGSGEALGALVVLAVNVT
                     LLILAGSGTLWVQRWLATRRNHD"
     gene            complement(1708..2655)
                     /locus_tag="VFB52_02240"
                     /pseudo
     CDS             complement(1708..2655)
                     /locus_tag="VFB52_02240"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
     gene            complement(2678..3157)
                     /locus_tag="VFB52_02245"
     CDS             complement(2678..3157)
                     /locus_tag="VFB52_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZO05178.1"
                     /translation="MAAKRSAGILLYRRNGGEPEFLLVHPGGPYWAKRDAGAWTIPKG
                     EVEPGEQTRDCALRELREELGPAPALAPEELIELGQIKQKSGKTVTAWAAEGEFDPAA
                     LDSISFAIEWPPRSGKEQEFPEVDRAGWFCATRAREKILPAQSPFIDRLLEHLSEAG"
     gene            complement(3161..3772)
                     /locus_tag="VFB52_02250"
     CDS             complement(3161..3772)
                     /locus_tag="VFB52_02250"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZO05179.1"
                     /translation="MSSSGDNATEERARAVRARRPHDAEASRAALLSAAREVFREVGY
                     ERATTRAIGERAGVDPALISRYFRSKEGLFMAAIAEMDSAETEIDYEPQTFVAYMLEY
                     WEERGHSPVSRALVSPALTEDVRRQVSAMVGELLVKGEAEALRERGVDDPKLHAELLV
                     ALAAGVAMTRANGTLETLSKASREEVLEVLAPLVDALLGGGQK"
     gene            3766..5208
                     /locus_tag="VFB52_02255"
     CDS             3766..5208
                     /locus_tag="VFB52_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05180.1"
                     /translation="MTSAADRTTSHSPRQHYGVTFAVLALAGASYAMLQSMVAPALPA
                     IQDELGASSSATAWIFTGFLLSASVLTPIVGRLGDMFGKARTLVIVLAVLSLGVLLAA
                     LATSVEMLIVARVIQGGGGALFPLAFGIIRDEFPAHRAATGIALISAILGIGGGLGIV
                     LAGPIVEGLDYHWLFWFPLVLCLLATVSAHFFVPESPVRSPGKVDWGGAALLSAWLVL
                     VLLGISQTADWGWEDPRVIGLIAAGLVVAVIWARYEERKREPLVDMVMMRIRGVWTVN
                     AAAFLVGAGMYSSFILVPEFTETPASTGYGYGASVTEAGLYLLPATLAMMIVSPIAGS
                     LATRIGPRITLIGGCLVTTLSFTLMAVAHTAHFNMYVATALMGVGIGLAFSSLANLIV
                     EAVPPEQTAIATGMNTVMRTLGGSVGGQVGASLIAGTVVASSLPTENGFTLAFGFAAG
                     ACLLAALASMAVPRRSGPKFKPIPIPAGAD"
BASE COUNT          701 a         1942 c         1821 g          899 t
ORIGIN      
        1 ttcttcgggg tggttagcgg ggaggggtcg gggtgtccct tccacttaac ttttttgggg
       61 atcgagcggc ggctagacgc ctcgattcgc tgacacaaaa aagtacaaga cgttccgagg
      121 ggtacccgac ccctccactc aagccgcgcc ggaggttccg tcggcagcga gaagggcgag
      181 cttgcctcag gcggcgagtg tcgacttttg cgtgctagag gccgcaaaag tcgacactcg
      241 cgtcccacgg gccgcttatg ttgagcaggt cccgtatctg ttctgctcgc attagggaaa
      301 ggggttgctc atgaacgaac cggccacgat aagcggttgg tgggttttcg ctggagtcct
      361 gctgatcgtc gcggggctgc tcaacatcgt ctgggggatt gccgcgatcg gcgactcgaa
      421 gttcttcacc gaccacggca ccttcatcct cgccagcctc aacacctggg gttggatcat
      481 gttgatcgtc ggcgtgctcg agatggtcgc cgcgttctcg ctgtttgccg ggggcgaatt
      541 cggccgctgg ttcgggatct tcatcggcgc ggtcaactcg gtcggcgcgc tgctctcgat
      601 ccccgcttac ccgttctggt cgctggcggt tttcgcgctc tcgatcatca tcatctacaa
      661 gctcgcggag ggaccggaag gatcggtttg acgccgctgc ggtgagaaga atggagcgcg
      721 gtgctgatgc tgcgctcatc cgtggaggcc gagctggtcc cccgcttcgc cggtctgctg
      781 cgcgacatcg acggtgtccg tcgggtcgtg cagcagccgg acgagtccga agagcgcggc
      841 tgtagcgtct tcgtcgccga cgtcgaaccg gccgccgccg accgcctggt cgaggcgatc
      901 gccgagctcg gcatcccggt cgacgactac gtgctcagca agatcgacgt ggtcgcgccg
      961 ctgcgccgcc agctcggcgg cgatcacggg atcgagggct tcgcctgggt cgaggtgatg
     1021 ggccaggcgc gggccaactc gcgcccgctg gcccgctacc tggcgctgat cagcgtcgcc
     1081 gcggtgatcg ccgcctgcgg cgtgatcacc agcagctcga tcctgatcgt cggcgcgatg
     1141 gcgatcagcc ccgacgtgct gccgatctgc gccacctgcg tcggcatcgt cggcgggcgc
     1201 cggcggttgg ccgaacgctc ggcgatcacg ctgacgctcg gcctcgtcct ggtcgtggtc
     1261 gtgaccgcgg tgctctcggc cctgctcaac gccggtgact tcctgcccag cggcttcaag
     1321 gtcgaggact cggccctgag cacgctcgcc cacaccgact actcgaccgt gctggtcgcg
     1381 ctggcggcgg gggtggcggc gatgctggcc ttcgagacgc gggcgagcgc cgcggtcggc
     1441 gtcgcgatct cggtgacgac gatgcctgcc tcggcctacc taggcgtctc gatcggtgcc
     1501 ggcggttccg gcgaagcgct cggtgccctg gtcgtgctcg cggtcaacgt caccctgctg
     1561 atcctcgccg gcagcggcac cctctgggtg cagcgctggc tcgctacccg ccgcaaccac
     1621 gactgaagcg ggcgagggcg cgggctgcga ccacggcgcc gggtcggggc ggcgctcgtc
     1681 gtagaccacc tcggtgccgg agaagcggtc ttcccaaccg cgccggtttt cgcggaagac
     1741 cacgccgagg aagccgatgc cgagggtgac cacggagaga tagaggccga gcagtcgttt
     1801 cagcccacgc cgcagcgaca gccgccgctc ggtcaggcgg atccccagga aacgcatccc
     1861 cggggtctgg ccggccagcg cccagaagac gagcaagtag gaggacccga tcgccaccca
     1921 ggcggtcgag ccgaccgcga tcgcccagtt ggaggcgccg ccgttgccgc caccgaaggc
     1981 gttgtcgagg agggtgagca gggcgaccag ggcggtgaag ccgaggttga cgaagacgcc
     2041 gtcgatcacg taggcgaggg cacggctgac gaagccggcg tcgggactgg gcagctcccg
     2101 ccgcttgcgg aagaagatgc gccggacgat ccgctcgaag ctgtcgtcga ggtcgcgggc
     2161 gatcttgccg atcgtgcggc cgatgtcctc gatcaggccg gccccctggg cgctgatcgc
     2221 ggcgcgcagc tcgggcgccc cggcgatccg ctcgaccagc tgctgggcct cctcgctggc
     2281 cagcagccgc cgccagacct cgtcgaccag ctcgctgtcc atcgcctcga gcagcgcccg
     2341 cttgaccgcg tcgctctcca gcgccccctg gaccgcctct tcgaccaccg ggccgttcag
     2401 cacccgggcc atcgcccgct cgaccgcctc gctttcgatc gcgccgacga tcgcctcctc
     2461 ggcggcgacc tcaactgcac ggtcgatccc ggtcgccttg ccgacgctgc gcgcgccgcg
     2521 cacgccgacc ccgagcagac gcgcggagat cggcaggtgc ccgttggcgc tgccgttggg
     2581 cgaggcgccg ttcgcggcgg cgccgttggc gctcgcgtcg gtgcccgcgg accgctttgc
     2641 ggagccgttt tccatcgccc cgcgagtata agtcggctca gccggcctcg ctcaaatgtt
     2701 cgagcaggcg gtcgataaac ggcgactgcg ccggcaggat cttctcccgc gcccgggtcg
     2761 cgcagaacca tccggcgcga tcgacctcgg ggaactcctg ctccttaccg ctgcgtggcg
     2821 gccactcgat cgcgaacgag atgctgtcca gcgccgccgg gtcgaactcg ccttcggcgg
     2881 cccaggctgt gaccgtcttg cccgacttct gtttgatctg acctagctcg atcagctcct
     2941 ccggagccag cgccggggcc gggccgagct cctcgcgcag ctcgcgcagg gcgcagtcac
     3001 gcgtttgctc gccgggctcg acttcgccct tcgggatcgt ccaggcgccg gcgtcgcgct
     3061 tggcccagta gggcccgccc gggtggacga gcaggaactc gggctcaccg ccgttgcggc
     3121 ggtagagcag gatgccggca ctgcgttttg cggccatcga ctacttctgc cccccgccca
     3181 agagggcgtc gaccagcggc gccagcacct cgaggacttc ctcacgggag gccttggaga
     3241 gcgtctccag ggtcccgttg gctcgcgtca tcgccacccc ggcagcgagc gccacgagca
     3301 gctcggcgtg cagcttcggg tcgtcgacac cgcgctcgcg cagcgcttcc gcctcgccct
     3361 tgaccagcag ctcgccgacc atcgcgctga cctggcgacg cacgtcctcg gtcagcgccg
     3421 ggctgaccag ggcgcggctg accgggctgt ggccgcgctc ctcccagtac tcgagcatgt
     3481 aggcgacgaa ggtctggggc tcgtagtcga tctcggtttc cgccgagtcc atctcggcga
     3541 tcgcggccat gaacaggcct tctttggagc ggaagtagcg ggagatcagg gccgggtcga
     3601 cgcccgcccg ttcgccgatc gcgcgggtcg tggcgcgctc gtagccgacc tcgcggaaga
     3661 cctcgcgggc ggcgctcagc agcgcggccc ggctggcctc ggcgtcgtgc gggcggcgcg
     3721 cgcggacagc ccgcgcacgc tcctcggtag cgttgtcacc gcttgatgac atctgcagcc
     3781 gaccgtacca cctcccactc cccgcgacag cactacggcg tcaccttcgc cgttctcgcg
     3841 ctcgccggtg cctcctacgc gatgctgcag tcgatggtcg ccccggcgct gccggcgatc
     3901 caggacgaac tcggcgcctc ctcttcggcg accgcctgga tcttcaccgg cttcctgctc
     3961 agcgcctcgg tcctgacccc gatcgtcggc cggctcggtg acatgttcgg caaggcgcgc
     4021 acgctggtga tcgtgctcgc cgtgctcagc ctcggcgtgc tgctggcggc gctcgccacc
     4081 tcggtcgaga tgctgatcgt cgcgcgcgtg atccagggcg gtggcggcgc cctcttcccg
     4141 ctcgccttcg ggatcatccg cgacgagttc ccggcccacc gcgccgccac cgggatcgcg
     4201 ctgatctcgg cgatcctcgg gatcggcggc ggcctcggga tcgtgctcgc cggcccgatc
     4261 gtcgaggggc tcgactacca ctggctgttc tggttcccgc tggtgctctg cctcctcgcc
     4321 accgtctccg cccacttctt cgtgcccgag tcgccggtgc gctcgcccgg caaggtcgac
     4381 tggggcggcg ccgccctgct ctcggcctgg ctggtcctgg tcctgctcgg gatcagccag
     4441 accgccgact ggggctggga ggacccgcgg gtgatcgggc tgatcgccgc cggcctcgtc
     4501 gtcgccgtga tctgggcgcg ctacgaggaa cgcaagcgcg agccgctggt cgacatggtg
     4561 atgatgcgaa tccgcggcgt ctggacagtc aacgccgccg ccttcctggt cggggcgggg
     4621 atgtacagct cgttcatcct cgtgcccgag ttcaccgaga ccccggctag caccggctac
     4681 ggctacggcg ccagcgtcac cgaggccggc ctctacctgc tgccggcgac cctggcgatg
     4741 atgatcgtca gcccgatcgc cggctcgctg gcgacccgga tcggcccgcg gatcaccctg
     4801 atcggcggtt gcctcgtgac caccctttcg ttcacgctga tggcggtggc ccacaccgct
     4861 cacttcaaca tgtacgtggc gacggccctg atgggggtcg ggatcggcct cgccttctcc
     4921 tcgctcgcca acctgatcgt cgaagcggtg ccgcccgagc agaccgcgat cgcgaccggg
     4981 atgaacacgg tgatgcgcac gctcggcggc tcggtcggcg ggcaggtcgg cgccagcctg
     5041 atcgccggca ccgtggtcgc ctccagcctg ccgaccgaga acggcttcac cctcgccttc
     5101 ggcttcgcag ccggcgcctg cctgctcgcc gcgctcgcca gcatggcggt accgcgccgc
     5161 tcaggcccta aattcaagcc aatcccgatc cccgctggag ccgactgatg tccctctacc
     5221 gcaacatcct cgtcgcgatc gacggctcag agcacggcgc cgcggcgctg cgcacggccg
     5281 cccagctcgc ccgcgaggag cacgcgcgcc tgaccgtcgt caccgcggtg ccgccggcgc
     5341 cggccttcgc ccagctcacc gcc
//
LOCUS       DASRIG010000084         5231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2382451, whole genome shotgun sequence.
ACCESSION   DASRIG010000084 DASRIG010000000
VERSION     DASRIG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382451"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFB52_02260"
     CDS             <1..1152
                     /locus_tag="VFB52_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO05181.1"
                     /translation="KFHWFLPTQGDGRALIETGGTGPTGTAGGLRDSSIPYLSRIATA
                     AEDLGFEAALTPTGGWCDDAWVVTAMLSETSERLKFLIAFRPGMVAPPLAAQMAATFQ
                     WHSGGRLLLNVVTGGDSEEQRAYGDFLGKDERYRRTGEFLEIVRRLWAGETVDFAGEH
                     LQVEGAALHRVPDPAPQIYFGGSSPAAGEVAAEHVDVYLTWGEPPEAVAEKVAWIQAL
                     AAERGRRVRFGIRLHVIARDSAAEAWAEAERLLSTLDAETIARVQDKLQRSESVGQKR
                     MLDLHGGSAADLEVAPNLWAGVGLGRGGAGTALVGSHEEIADRIEEYANVGIEEFVLS
                     GYPHLEELYWFGEGVLPVLRERGLWREPGFEASARGREGNAVPFAVGAR"
     gene            1149..2618
                     /locus_tag="VFB52_02265"
     CDS             1149..2618
                     /locus_tag="VFB52_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZO05182.1"
                     /translation="MSTPDATRNGTPPAEAEIRLRREPRTRVAVIGGGAAGTLSAVHL
                     MRRVEEGGVEVSLIDAKGDFGPGLAYGTDDPRHLLNVPACRMGAISGHPEHFHEWVRE
                     RGGESGPEEFLPRGLFGTYLRELLDGTEAACTRRARLDRLHAEVTGLNVPHSPQAPLA
                     LDLADGSRLEADQVVLALGPLPGGDPIPVPEELRENGTYIADPWAPGALDPVRANERV
                     LIIGTGLTMVDVVLSLAGGAGGGPRVHAVSRHGLVPRRHRREITQIKRFPVPLEDGRL
                     EPVLAAVFEQIARVSQQGGDWRDVFDSMRLATPTIWRSLQIEEKRRFLAGVARLWDVH
                     RFRMAPDVADRFEELQADGRLRVDARAIVALDPRQRGIGVSLHTVGREELEIAEVDRV
                     INCSGAGNSLARGATPLVRDLLASGTARPEPLGLGLDVAEDGGLIDATGRVSERIRVV
                     GSLRKGVEWEAIGVTEIRDHAEAVAAALFATVERPQRVA"
     gene            2631..3227
                     /locus_tag="VFB52_02270"
     CDS             2631..3227
                     /locus_tag="VFB52_02270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZO05183.1"
                     /translation="MGKRIRLLILLGSVTAPGRLQRALADAVARSGAENWIEAELIDL
                     ADYRLDFVGRPGEAEDDREQLVEAVAEADAVVFATPVYRGSMSGALKNLFDLLPVEAL
                     ESKPAGIVAMGASAHHFLGAERHLRDVLSFFGALQAPVAVYLTSADFDEGVPTERAEA
                     ALDELFAGVGSIAMALPTAWQLGPQPLAARKVPVVPTS"
     gene            complement(3236..3868)
                     /locus_tag="VFB52_02275"
     CDS             complement(3236..3868)
                     /locus_tag="VFB52_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05184.1"
                     /translation="MSTSQHTPVVPLEAGAGPDNPPCPACGEPLFGWLAPQPGLAGAI
                     SRCESCGLGVVGGEASPEEALAELDRLRDGDCIRISNRASWAGSLGSAGWAGLTPGTR
                     YLFTVEAVRRLIAHRDQVVASCRWAPGASFANTWQTLLNSVTFGHNVALGALRGNAVA
                     APAKKGWQRKIDALASIVLAIPAAIIAAPIELIAGPTRRGAVLRIKPQLL"
     gene            3889..4848
                     /locus_tag="VFB52_02280"
     CDS             3889..4848
                     /locus_tag="VFB52_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05185.1"
                     /translation="MTKSAILSRERAWFTRVGVAGILGGILALVGFVLLQSAIGGDAN
                     FESLTEAKDNSSNVWIAGVLTGVGYLLVAAPLYFLFRAAQARSDRVKNQMVGLVLLGP
                     LLLGISAPLISGGTQEAADSYIAGSVKPDKTTAEIKEECEEDKTDKSAEDFSKDFPEV
                     DGKTGLAACEDKKTEESKASQAIKDSTLLKIGQFAGLAGGLSLVVGLLYVGLWTMRTG
                     LLSRFWGSLGMAVGFASIIGFSPLTFIWFIFVGLLLAGFLPGGRPPAWDAGEAVPWPS
                     PGEKAAAEMEPDDDAEVVDVDSEEIDETEIPTNGKRKRKRRSE"
     gene            complement(4930..>5231)
                     /locus_tag="VFB52_02285"
     CDS             complement(4930..>5231)
                     /locus_tag="VFB52_02285"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZO05186.1"
                     /translation="ATAGLTELAPYALSLAFRIRYTLDLNAREAMHLIELRSGREGHP
                     GYRAVAQAMHERIAAVHPGVAAAMNFVDTSAEPRLERIMSEIRTHRKQIAAGRSE"
BASE COUNT          729 a         1910 c         1890 g          702 t
ORIGIN      
        1 aagttccact ggttcctgcc cacccagggc gacggccggg cgctgatcga gaccggcggc
       61 accggcccga ccggcaccgc cggcggcctg cgcgactcct cgatccccta cctctcgcgg
      121 atcgcgaccg cggctgagga cctcggcttc gaggcggcgc tgaccccgac cgggggctgg
      181 tgcgacgacg cctgggtggt caccgcgatg ctcagcgaga cctccgagcg gctcaagttc
      241 ctgatcgcct tccgccccgg gatggtggcg ccgccactgg ccgcgcagat ggcggcgacc
      301 ttccagtggc actcgggcgg ccgcctgctg ctcaacgtgg tgaccggcgg cgatagcgag
      361 gaacagcgcg cctacggcga cttcctcggc aaggacgagc gctaccggcg caccggcgag
      421 ttcctcgaga tcgtgcggcg cctgtgggcg ggggagacgg tcgacttcgc cggcgagcac
      481 ctgcaggtcg agggcgcggc cctgcaccgg gtgcccgacc cggcgccgca gatctacttc
      541 ggcggcagct ccccggccgc cggcgaggtc gccgccgagc acgtcgacgt ctacctgacc
      601 tggggcgagc cgcccgaggc ggtcgccgag aaggtcgcct ggatccaggc gctggccgcc
      661 gagcgcgggc gacgggtgcg cttcgggatc cgtctgcacg tgatcgcgcg ggactccgcc
      721 gccgaggcct gggccgaggc cgagcggctg ctctcgactc tcgacgccga gacgatcgcc
      781 cgcgtccagg acaaactgca gcgctcggag tcggtcggcc agaagcgcat gctggacctg
      841 cacggcggct ccgccgccga cctcgaggtc gcccccaacc tctgggccgg ggtcgggctc
      901 ggccgcggcg gcgccggcac ggcgctggtc ggcagccacg aggaaatcgc cgaccggatc
      961 gaggagtacg cgaacgtcgg gatcgaggag ttcgtgctct ccggctaccc gcacctggag
     1021 gagctctact ggttcggcga gggcgtgctg ccggtcttac gcgagcgcgg gctctggcgc
     1081 gagccgggct tcgaggcgag cgcccgcggg cgcgagggca acgcggttcc gttcgcggtg
     1141 ggtgcacggt gagcacgccg gacgccaccc gcaacggcac cccgccggcc gaggcggaga
     1201 tccggctgcg ccgggaaccg cggaccaggg tcgcggtgat cggcggcggc gccgccggca
     1261 cgctcagcgc cgtgcacctg atgcgccggg tcgaggaggg cggcgtcgag gtcagcctga
     1321 tcgacgccaa gggcgacttc ggccccggcc tcgcctacgg caccgacgac ccccgccacc
     1381 tgctcaacgt gcccgcctgc cggatgggcg cgatctccgg ccaccccgag cacttccacg
     1441 agtgggtgcg cgagcgcggc ggcgagtccg ggccggagga gttcctgccg cgcggcctct
     1501 tcggcaccta cctgcgcgag ctgctcgacg gcaccgaggc cgcgtgtacg cggcgggcgc
     1561 ggctcgaccg gctccacgcc gaggtcaccg ggctgaacgt cccgcactcg ccgcaggcgc
     1621 cgctggcgct cgacctcgcc gacggctccc ggctggaggc cgaccaggtg gtgctggccc
     1681 tgggcccgct gcccggcggc gacccgatcc cggtgcccga ggagctgcgc gagaacggca
     1741 cctacatcgc cgacccctgg gcgcccggcg cgctcgaccc ggtgcgcgcc aacgagcgcg
     1801 tgctgatcat cggcaccggg ctgaccatgg tcgacgtcgt gctcagcctc gccggcgggg
     1861 ccggcggcgg cccgcgcgtc cacgcggtct cccgccacgg cctcgtgccg cgccgccacc
     1921 gccgcgagat cacccagatc aagcgcttcc cggtgccgct cgaggacggg cgcctggagc
     1981 cggtcctcgc cgccgtcttc gagcagatcg cccgggtctc ccagcagggc ggcgactggc
     2041 gcgacgtctt cgactcgatg cggctggcga cgccgacgat ctggcgctcg ctgcagatcg
     2101 aggagaaacg ccgcttcctc gccggtgtgg cgcgcctctg ggacgtgcac cggttccgca
     2161 tggcgccgga cgtcgccgac cgcttcgagg agctgcaggc cgacggccgg ctgcgcgtcg
     2221 acgcgcgcgc gatcgtcgcc ctcgacccgc gccagcgcgg gatcggcgtc tccctgcaca
     2281 cggtcggccg cgaggagctg gagatcgccg aagtcgaccg ggtgatcaac tgcagcggcg
     2341 caggaaactc gttagcgcgg ggggccaccc cgctggttcg cgatctgctc gccagcggta
     2401 ccgcccgccc cgagccgctc ggcctcggcc tcgacgtcgc cgaggacggc ggcctgatcg
     2461 acgccaccgg ccgcgtctcc gaacggatcc gcgtggtcgg ctcgctgcgc aagggggtcg
     2521 agtgggaggc gatcggcgtg accgagatcc gcgaccacgc cgaagccgtc gccgccgcgc
     2581 tcttcgccac cgtcgaacgt ccccaacgag tcgcctgagg aggccaaacc atgggtaagc
     2641 gcatccgcct tctgatcctg ctcggcagcg tcaccgcgcc gggccgcctg cagcgcgcgc
     2701 tggccgacgc ggtcgcgcgc agcggcgccg agaactggat cgaggccgag ctgatcgacc
     2761 tcgccgacta ccggctcgac ttcgtcggcc gtccgggcga ggccgaggac gaccgcgagc
     2821 agctggtcga ggcggtcgcc gaggccgacg ccgtcgtctt cgcgacgccg gtctaccggg
     2881 gctcgatgag cggcgcgctg aagaacctct tcgacctcct gccggtcgag gcgctcgaat
     2941 cgaagccggc cgggatcgtc gcgatgggcg cctccgcgca ccacttcctc ggcgccgagc
     3001 gccacctgcg cgacgtgctc tccttcttcg gcgccctgca ggcgccggtc gccgtctacc
     3061 tgaccagcgc cgacttcgac gagggcgtcc cgaccgagcg cgccgaggcc gccctcgacg
     3121 agctcttcgc cggcgtcggc tcgatcgcga tggccctgcc caccgcctgg cagctcggcc
     3181 cgcaaccgct ggccgcccgc aaggtcccgg tggttccgac cagctaggcg tgcggctaaa
     3241 gcagctgcgg tttgatccgc agcacggcgc cgcgccgcgt cgggccggcg atcagctcga
     3301 tcggtgcggc gatgatcgcg gcggggatcg cgagcacgat gctggcgagg gcgtcgatct
     3361 tgcgctgcca ccccttcttg gcgggagccg cgaccgcgtt gccgcggagc gcgccgaggg
     3421 cgacgttgtg gccgaaggtg acgctgttca gcagcgtctg ccaggtgttg gcgaagctgg
     3481 cgccgggggc ccagcggcag ctggcgacga cctggtcgcg gtgggcgatc agccgccgca
     3541 ccgcctcgac ggtgaacagg tagcgggtgc ccggcgtcag cccggcccag ccggcgctgc
     3601 cgagcgagcc ggcccagctg gcgcggttgg agatccggat gcagtcgccg tcgcgcaggc
     3661 ggtcgagctc ggccagggcc tcctcggggc tggcctcgcc gccgaccacg ccgaggccgc
     3721 agctctcgca gcggctgatc gcccccgcca ggcccggctg tggcgccagc cagccgaaca
     3781 gcggctcgcc gcaggcgggg cagggtgggt tgtcgggacc ggctcccgcc tcgagcggga
     3841 ccacgggggt gtgctgcgaa gtgctcatag tcggatagcg tacgcgccgt gaccaagtcg
     3901 gcgatcctct cccgcgagcg cgcctggttc acccgggtcg gcgtcgcggg catcctcggc
     3961 ggcatcctcg cgctggtcgg cttcgtgctg ctgcagagcg cgatcggcgg cgacgccaac
     4021 ttcgaaagcc tcaccgaagc gaaggacaac agctccaacg tctggatcgc cggcgtgctc
     4081 accggggtcg gctacctgct cgttgcggcg ccgctctact tcctcttccg cgccgcccag
     4141 gcgcgcagcg accgggtcaa aaaccagatg gtcgggctcg tcctgctcgg cccgctgctg
     4201 ctcggcatct ccgccccgct gatctccggc ggcacccagg aagccgccga cagctacatc
     4261 gcaggcagcg tgaagccgga caagaccacg gcggagatca aagaggagtg cgaagaagac
     4321 aaaacggaca agagcgccga ggacttcagc aaggacttcc ccgaggtcga cggcaaaacc
     4381 ggcctcgccg cctgcgaaga caaaaagacc gaggaaagca aggcctcgca ggcgatcaag
     4441 gactcgacgc tgctgaaaat cggccagttc gccggcctcg ccggcggcct ctcgctggtc
     4501 gtcggcctgc tctacgtcgg tctctggacg atgcgcaccg gcctgctcag ccgcttctgg
     4561 ggatcgctcg ggatggcggt cggcttcgct tcgatcatcg gcttctcgcc cctgaccttc
     4621 atctggttca tcttcgtcgg gctcctcctc gccgggttcc tgccgggcgg ccgaccaccc
     4681 gcctgggacg ccggcgaagc ggtcccctgg cccagcccgg gcgaaaaagc cgcggcggaa
     4741 atggaaccgg acgacgacgc tgaagtcgtc gacgtcgact cagaggagat cgacgagacc
     4801 gagatcccga ccaacgggaa acggaagcgc aagcgacggt ccgagtaggg gctccgctta
     4861 acttttttga gaagcgagcg cgagccgtcc gagccgcgct gaagcaaaaa agtacaagac
     4921 gctccgcccc tactcggacc gtcccgcggc gatctgttta cggtgggtcc ggatctcgct
     4981 catgatgcgc tctaggcggg gttctgcaga cgtatccacg aagttcatcg cggccgcgac
     5041 gccgggatgc acggcggcga tccgctcgtg catcgcctgg gccaccgcgc ggtagccggg
     5101 gtggccctcg cggccggagc gcagctcgat caggtgcatc gcctcgcgcg cgttgaggtc
     5161 gagcgtgtag cggatgcgga aggcgaggct gagcgcgtag ggcgccagct cggtcaggcc
     5221 agccgtggcc a
//
LOCUS       DASRIG010000085         5132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2912143, whole genome shotgun sequence.
ACCESSION   DASRIG010000085 DASRIG010000000
VERSION     DASRIG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912143"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFB52_02290"
     CDS             <1..1137
                     /locus_tag="VFB52_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO05187.1"
                     /translation="VVQGRRSERDSRGGLRVRPNDLPNHQRPGVALVGATDDGRPSGL
                     AITDELLRQLADIKTDGNIMPPPSMNVERRTLLGGDVAVIVVQPADSPPVRYRGRIHI
                     RIGPRRGIATAQDERNLTEKRRHRDLPYDVHPLPSSTSKDLRRTFFEEVYLPAAVAPD
                     VLEANDRTYEQRLAATKMVFSVDDPRPTVLSILVIGKRTRDLIPGAYIQFLRVAGTDL
                     SDPIVDELAIDGVVSEALRRLDDKLLAHNRTAVDFTNGPVETRKHTYPLAALQQLIRN
                     AVMHRAYEATNAPVRVYWYDDRIEIINRGGPYGEVTVETFGTPGLTDYRNPNLAEAMR
                     VLGLVQRFGAGLPIARRALAENGNPPPEFSVRPTFVSATVRPSI"
     gene            complement(1149..2546)
                     /gene="radA"
                     /locus_tag="VFB52_02295"
     CDS             complement(1149..2546)
                     /gene="radA"
                     /locus_tag="VFB52_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZO05188.1"
                     /translation="MAKARTEYVCRECGAKAVRWSGRCPECQAWSTLEEKVVRATPAV
                     SANPAARPRMAVVPGVGGAARPLRLAEIDVNDFDRLPVGIEEFGRVLGGGIVRGSLVL
                     IGGDPGVGKSTLLAQVCDEVAKRNGPALYISGEESLAQIGLRARRLGLDAKDLLFASD
                     TDLGTILETIENARPSMVVVDSIQSIYSADVESSPGSVSQLRECTLRLMQLAKGSGVS
                     VFLIGHVTKEGALAGPKMLEHMVDTVLYLEGERYHTYRLLRGTKNRFGPTHEVGVFEM
                     RGEGMVEVANPSAAFLSERGEGATGSSVLVTMEGTRPLLVEVQALASRSSLAVPRRAA
                     NGIENNRLLLVSAVLGRRLGLPLHDQDLYVNVIGGLRIDEPAADLAVALSIVSSFRDR
                     QVDPRAVVAGEIGLSGELRSVGQLEIRLREAAKLGFERAVVPRSTALRSLKGLGLDLM
                     PAGTLREAINVALRE"
     gene            complement(2729..>5132)
                     /locus_tag="VFB52_02300"
     CDS             complement(2729..>5132)
                     /locus_tag="VFB52_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZO05189.1"
                     /translation="NYIGTEHLLLGLVREGEGVAAKVLANLGVELNKVRSAVEFIIGR
                     GDRTVTGDIGLTPRAKKVIELSVDEARRLNHHYIGTEHLLLGLVREGEGIAAGVLESL
                     GVSLDKVRSQVIYVLNQSQSYSQQESRHASKTPVIDQLGMDLTAAARANKLDPVIGRA
                     REIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRISTGDVPETLMGKRLLTLDI
                     GSLVAGTKYRGEFEERLKKIIEEVKNSGNCVLFIDELHTLVGAGAAEGAVDAANILKP
                     SLARGELQTIGATTLDEYRKYIERDAALERRFQPVLVEEPSVEETIEILKGIVERYEQ
                     HHRLEISEEALKAAAELAARYVPDRFLPDKAIDLVDEAASRVRMQRASAPPSYKEMLR
                     SLESVQNEKEAAISAQQYELAAEYRDQEAKLRAKLEKIESGWQTQQSADKPVVTAEDI
                     AHVVAMWTGIPVMRIAEEESSRLLKMEEALHDRIKGQDEPITMVAKAVRRARAGLKDP
                     RRPIGSFIFLGPTGVGKTELVRALAEFMFGSEEAMIRIDMSEFMERHSVARLVGAPPG
                     YIGYEEGGQLTEAVRRKSYSVVLLDEIEKAHPEVFNILLQIMDDGRLTDAKGRKVDFR
                     NTILIMTSNVGAELIRRESVLGFNTPRDLAKDAQGAYERMKDKVLTEMKKLFRPEFLN
                     RIDSTIVFSALAQEQVREIIDLMLKRVQTQLTEQRMTLEVTDGAKDVLMKTGFDQIYG
                     ARPMRRAIMVAIEDPLAEGLLHGTFQPGDRVVADAKDGEIVLDVKEREAPEPEPEPAA
                     TA"
BASE COUNT          766 a         1891 c         1610 g          865 t
ORIGIN      
        1 gtcgttcagg ggcgacgctc cgaacgcgat tcgcgaggcg gtctgcgcgt tcgccccaac
       61 gatctgccga atcatcagcg tcccggggtc gccttggtgg gcgccacgga tgacggccgt
      121 ccatccggtc tcgcgatcac cgacgagctg ctccgtcagc tcgccgacat caagaccgac
      181 ggcaacatca tgccgccgcc ctcgatgaat gtggagcgtc ggacgctgct cggtggagac
      241 gtcgccgtta tcgtcgttca gccggccgac tccccgccgg ttcggtaccg cggccgcatc
      301 cacatccgga tcgggccacg ccgggggatc gccacggcac aggacgagcg caacctgact
      361 gagaagcgac gccatcgcga cctgccctac gacgtgcacc cgttgccatc gtcgacatcg
      421 aaggatctcc ggcgtacgtt cttcgaggag gtctacctgc cggccgccgt cgcccccgac
      481 gtgctggagg cgaacgatcg gacgtacgag cagcgcctcg ccgccaccaa gatggtcttc
      541 tcggtcgacg acccaaggcc caccgttctc agcatactgg tgattggcaa gcgcacccgc
      601 gacctgattc cgggcgccta catccagttc ctcagagtcg ccggcaccga tctgtccgac
      661 ccgatcgtcg acgagctggc gatcgacggc gtcgtgagcg aggcgctccg ccgcctggac
      721 gacaagttgc tcgcgcacaa tcgcacggcc gtggacttca ccaacggacc cgtcgagacc
      781 aggaagcata cctatcccct ggcggcgctt cagcagctca tcaggaacgc cgtgatgcac
      841 cgtgcctacg aggcgaccaa cgcccccgtg cgcgtctact ggtacgacga ccgcatcgag
      901 atcatcaacc gcggcggtcc gtacggcgag gtcaccgtcg agacgttcgg cacgcccggc
      961 ctgacggact accggaaccc gaacctcgcc gaggcgatgc gcgtgctcgg cctggtccag
     1021 cggttcggcg caggtctccc gatcgcccgc cgagcgctcg ccgagaacgg caacccacca
     1081 ccggagttca gcgtgcggcc gacattcgtc tcggccacgg ttcgtccgtc aatctgagcg
     1141 agcgaccgtc attctcggag ggccacgttg atcgcctcgc ggagggtgcc ggccggcatg
     1201 aggtccagcc ccagcccctt cagcgaccgc aacgccgtcg agcgcggcac cactgcccgc
     1261 tcgaagccga gcttggccgc ctcgcgcagc cggatctcca gctgcccgac gctccgaagc
     1321 tcgcccgaga ggccgatctc gccggccacc accgctcgcg ggtcgacctg ccgatcccgg
     1381 aagctcgaca cgatcgagag cgccaccgcc aggtccgcgg ccggctcgtc gatgcggagg
     1441 ccgccgatca cgttgacgta caggtcctgg tcgtgcagcg gcaggcccag gcgccggccg
     1501 agcactgccg agaccagcag cagccggttg ttctcgatgc cgttcgccgc ccggcgcggc
     1561 accgccagcg agctgcggct ggcgagcgcc tggacctcga ccagcaacgg cctggtcccc
     1621 tccatcgtca ccagcaccga cgagccggtc gcgccctcgc cgcgctcgga taagaacgcc
     1681 gccgagggat tcgccacctc gaccatcccc tcgccgcgca tctcgaacac cccgacctcg
     1741 tgggtcggcc cgaaccggtt cttggtcccc cgcagcagcc ggtaggtgtg gtaccgctcg
     1801 ccctctagat acaggacggt gtccaccatg tgctccagca tcttcgggcc ggccagcgct
     1861 ccctccttgg tgacgtggcc gatcaagaag acgctgacgc cgctcccctt cgcgagctgc
     1921 atcaggcgca gggtgcactc ccgaagctgg ctcacgctgc ccggcgacga ctcgacgtcg
     1981 gcgctgtaga tgctctggat cgagtcgacg acgaccatgc tcggccgggc gttttcgatg
     2041 gtttcgagga tggtcccgag gtcggtgtcg ctggcgaaca acagatcctt cgcgtccagg
     2101 ccgagccgcc tcgcccgcaa cccgatctga gccagcgact cctcgcccga gatgtacagc
     2161 gccgggccgt tccgcttcgc gacctcgtcg cagacctggg ccagcaacgt gctcttgccg
     2221 acgcccggat cgccgccgat cagcaccagc gagcctctga cgatcccgcc gccgagcacc
     2281 cgcccgaact cctcgatccc gaccggcagc cggtcgaagt cgttgacgtc gatctcggcg
     2341 agccggagcg gccgggcggc gccgccgacg cccggcacca cggccatgcg cgggcgcgcg
     2401 gccgggttgg cgctcaccgc cggggtcgcg cggacgacct tctcttcgag ggtgctccag
     2461 gcctggcact ccgggcagcg accactccag cgcaccgcct tcgcgccgca ctcccgacag
     2521 acgtactcag ttctcgcctt cgccatacgg tagcgccctc accccagccc tctcccggag
     2581 ggagagggag ccagatcctc tctccccctc tcccacggta gggagagggg gccgggggtg
     2641 agggccgtcc tccgccaccc ccaacatgtg aaagggggcg agccctgcgg cccgccccca
     2701 ctgcctactg cctactgccc tctgccgact acgcggtcgc ggccggctcc ggctcgggct
     2761 cgggagcctc gcgctccttc acgtcgagca cgatctcgcc gtccttcgca tcggccacca
     2821 cccggtcgcc cggctggaag gtgccgtgga gcaacccctc ggctagcgga tcctcgatcg
     2881 ccaccatgat cgctcgccgc atcgggcgcg cgccgtagat ctgatcgaag cccgtcttca
     2941 tcaggacgtc cttggcgccg tcggtgactt cgagcgtcat ccgctgttcg gtgagctgcg
     3001 tctggactcg cttgagcatc aggtcgatga tctcgcggac ctgctcctga gcgagggcgc
     3061 tgaacacgat cgtcgagtcg atccggttca ggaactcggg ccggaacagc ttcttcatct
     3121 cggtgaggac cttgtccttc atccgctcgt aggcgccctg cgcatccttc gcgaggtcac
     3181 gcggggtgtt gaagccgagg acgctctccc gccggatcag ctcggcgccg acgttcgagg
     3241 tcatgatcaa gatcgtgttc cggaagtcca ccttgcgacc cttggcgtcg gtcaaccgtc
     3301 cgtcgtccat gatctggagc aggatgttga agacctcagg gtgggccttc tcgatctcgt
     3361 ccagcagcac caccgaatac gacttgcgcc gcaccgcctc ggtgagctgg ccgccctctt
     3421 cgtacccgat atacccggga ggcgccccga cgagccgggc cacggagtgg cgctccatga
     3481 actccgacat gtcgatccgg atcatcgcct cttcgctgcc gaacatgaac tcggcgagcg
     3541 cccgcaccag ctcggtcttc ccgacgcccg tggggccgag gaagatgaac gagccgatcg
     3601 gccgacgcgg gtctttcagg ccggcccgcg cccggcggac cgccttggcg accatcgtga
     3661 tcggctcgtc ctgccccttg atccggtcgt ggagcgcctc ttccatcttg aggaggcggc
     3721 tcgactcttc ctcggcgatc cgcatcaccg ggatccccgt ccacatcgcg acgacgtgcg
     3781 cgatgtcctc ggccgtgacc accggcttgt cggcgctctg ctgggtctgc cagccggact
     3841 cgatcttctc gagtttggcg cggagcttgg cctcctggtc gcggtactcg gcggccagct
     3901 cgtactgctg ggccgagatc gcggcctctt tctcgttctg gacggactcc aggctgcgga
     3961 gcatctcctt gtaggagggc ggggcgctcg cgcgctgcat ccgcactcga gacgccgcct
     4021 cgtccaccag gtcgatcgcc ttgtcgggca ggaagcgatc cgggacgtag cgggcggcca
     4081 gctcggcggc ggccttgagc gcctcctcgc tgatctccag ccgatgatgc tgctcgtacc
     4141 gctcgacgat ccccttcagg atctcgatcg tctcctcgac gctcggctcc tcgaccagga
     4201 ccggctggaa gcgccgctcc agggccgcgt cccgctcgat gtacttgcgg tactcgtcca
     4261 gcgtcgtcgc gccgatcgtc tggagctcgc cgcgcgccag cgacggcttg aggatgttcg
     4321 ccgcgtcgac ggcgccctcg gccgcgcccg cgccgaccag cgtgtgcagc tcgtcaatga
     4381 agaggacgca gttgcccgag ttcttgacct cttcgatgat cttcttgagg cgctcctcga
     4441 actcgccgcg atacttcgtg cccgccacca gcgaaccgat gtcgagcgtc agcagccgct
     4501 tgcccatcag cgtctcgggg acgtcgccgg tcgagatccg ctgggccaga ccctcgacga
     4561 tggccgtctt gccgacgccc ggctcgccga tcagggccgg gttgttcttg gtccgccgcg
     4621 agaggatctg gatgacccgc tcgatctctc tcgctcgccc gatgacggga tcgagcttgt
     4681 tcgcccgtgc cgcggccgtc aggtccatgc cgagctggtc gatgaccggc gtcttgctgg
     4741 cgtggcgcga ctcctgctgc gagtagctct ggctctggtt caggacgtaa atgacctgac
     4801 tgcggacctt gtcgagcgaa acccccagcg actccaggac gccagcggcg atcccctcgc
     4861 cttcgcgcac caggccgagc agcaggtgct cggtcccgat gtagtggtga ttcagccgcc
     4921 gcgcctcgtc gacgctgagc tcgatcacct tcttcgcacg cggggtcagg ccaatgtccc
     4981 cggtgacggt acgatcgcct cggccgatga tgaactcgac cgcggaccgg accttgttca
     5041 gttcaacccc tagattggcg aggaccttcg ccgcgacgcc ttcgccctcg cggaccaggc
     5101 cgaggagaag gtgctcggtg ccaatgtagt tg
//
LOCUS       DASRIG010000086         6621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2383616, whole genome shotgun sequence.
ACCESSION   DASRIG010000086 DASRIG010000000
VERSION     DASRIG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383616"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFB52_02305"
     CDS             <1..235
                     /locus_tag="VFB52_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05190.1"
                     /translation="RAARRQRGNVSYVDVTERFAGHGLCGEDPWLYGIKLAASDGLIK
                     GSYHPRRSGQQAYAAAFATLLRQPAIRAALTLP"
     gene            complement(352..1518)
                     /locus_tag="VFB52_02310"
     CDS             complement(352..1518)
                     /locus_tag="VFB52_02310"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF005734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HZO05191.1"
                     /translation="MNEAQSAGVFPSQALAQAIDREWVSSGDYRIRPEAIQPASIDLR
                     LGDVAWALRCSFLPDVESSVEEKMEGLAFQKIDLRDGAVLERDRPYLVPLIEELALPD
                     DVRAKANPKSSTGRLDVFTRVITDRHHRFDDVRAGYHGKLYLEIVPRSFAIQVKTGLS
                     LNQLRLARGDVRLGDARIRELQAESPLIYLDADPVPEDEIAVSDGLFLSLDVSGPADR
                     VVGYRAKKNSLPVDLSKIRAYGWRDYWDPVYPEAGGRIVLEPEIFYLLLSAEGVSIPP
                     RIAAVMMAYDPTAGELRTHYAGFFDPGFGYDPSGGRHGSRAALEVRARDVSFMVEHRQ
                     PVCKLALERTSAPAERLYGQDLGSNYQGQVTMLSKHFEEQVGSGGAASPPKTSG"
     gene            complement(1515..2159)
                     /locus_tag="VFB52_02315"
     CDS             complement(1515..2159)
                     /locus_tag="VFB52_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05192.1"
                     /translation="MERLPRGRHGLSPEFVAQNQRERLITGLTEVLHEVGYQKTTVSL
                     IGQRAAVSKSDFYKHFESKDDCFYAAYETAVERIRERVVTACSAHPEDDWPPRVRDGI
                     SSLIDLLASDPPLAGITLVEGLRAGRGIYDRYQAALESFVPLIRDGAPAAPGGGEVPQ
                     ATDEAVVGGIASLLGRRALAGEIEQLEELRPAVLEFALTPYLGTAEARRIISAG"
     gene            complement(2205..2819)
                     /locus_tag="VFB52_02320"
     CDS             complement(2205..2819)
                     /locus_tag="VFB52_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05193.1"
                     /translation="MMKPTRPELPREFVAVHKQRRMIAAIAELSAEQGYEATKIADVV
                     SRAGVARKTLYDNFDGKEDLFLSAFDFYFAEARTSVEVACDSTGGGWQQRIESGLAAL
                     LRFVADQPAAARMCLVEALSATPRSSVSYDDAVRAFVELLKENSPEGEISLPETTEES
                     LVGGVAWILHQRIRAGETQKIASLLPELSRFVLSPYLGVTNSPK"
     gene            2952..3701
                     /locus_tag="VFB52_02325"
     CDS             2952..3701
                     /locus_tag="VFB52_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05194.1"
                     /translation="MSFDRIQLSQEILVAAIGEDWPQEQVDAAVELLANSDGKFPAGV
                     RKLPSELVKAVQRERLVVAMLMAAAELGYRETNVQDVIDRAGVSRPTFYEHFANKEDC
                     FLAAFDTSAARLRNKVDAAAERGGDSWRDRLRYSLEALIQFAVAEPQTARTLIVEARA
                     ASSDAVMRRVQLLEYFAECLDEDARQRLPEGEYSSVTAAGVIGGVEALLYSRLCKEDV
                     DNLEELLPSLMYFAVLPYEGHEVASEELALD"
     gene            3723..4154
                     /locus_tag="VFB52_02330"
     CDS             3723..4154
                     /locus_tag="VFB52_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05195.1"
                     /translation="MSSLAEMRSPQAARAALAFLLFSALLIGLTATVVPRTFYDDFPF
                     LASWVELLPPYNEHLVTDVGGLYLGFAVMFGCAAWTLERTLVRAVSVAWLLTAGIHLL
                     FHAGHLDNFSTADAVAELASLTLLLAPPPIAVWGTSPRPGA"
     gene            4170..4256
                     /locus_tag="VFB52_02335"
     tRNA            4170..4256
                     /locus_tag="VFB52_02335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4204..4206,aa:Ser,seq:cga)
     gene            4356..4721
                     /locus_tag="VFB52_02340"
     CDS             4356..4721
                     /locus_tag="VFB52_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HZO05196.1"
                     /translation="MSEQNSTTGGSVHHGEIDWEAAERSPEFRELERAKRSFVLPATI
                     FFLGWYVTFILLAGYAPSFMGESLIEGFTVGYALALSQFVMVWVLTAMYLRRADRDFD
                     PLAEKAVAKALEPRKGEER"
     gene            4718..6352
                     /locus_tag="VFB52_02345"
     CDS             4718..6352
                     /locus_tag="VFB52_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZO05197.1"
                     /translation="MSPAAPLAAVNVEAVAIFGVVVAVTLGITFWASRRTTSATQFWA
                     AGRQITGRQNGLAIAGDYMSAASFLGIAGLIFYFGFDGFLYSVGFLVAFLTVLFLLSE
                     RMRNAGKFTIADVLAFRLKERPARVAAALGTLAVVAFYLIAQMVGAGIIIEGLVGIDF
                     NVAVIATGAFMLVYVVFGGMLATTWVQIVKAILLMSATIVLSIFVLVKIGGNPIDIFN
                     DAKNQSEDPAAYLEPGLRFDNPIDTLSLGLGLVLGTAGLPHILMRFFTVPDAKAARNS
                     IGWAVGVIGVFYVMTTFLGFGARALLGEAGTTGAVDEAGNLAAPLLAQEVGGGAGSFG
                     GDAFLAVISAVAFATILAVVAGLVIAASGAVAHDVWSNVVRRGRDSEHEEVYVARIAA
                     LSIGAIAIAIAVIGGSDLNVSYMVGLAFAVAASANFPALLLALTWRRFNTTGAVSGVV
                     VGTVSAIFLVVTGPDVASDPLVDLTQPGIISIPLGFAACIIGTLLGRDETQAERSFDE
                     LHVRAETGLGAEVGEAARPTRQRPRQPVGAASASPD"
     gene            complement(6349..>6621)
                     /locus_tag="VFB52_02350"
     CDS             complement(6349..>6621)
                     /locus_tag="VFB52_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05198.1"
                     /translation="KLTCPAASTAPCTVAVKVKGAKKPKAALGSSQLKVAPGKTVSLT
                     GKLSKAGLKKLLAAGQLQAKIAVTGKVPGGETTLGTLAAKLVPKKR"
BASE COUNT          978 a         2282 c         2224 g         1137 t
ORIGIN      
        1 gagagcggca cgaaggcagc gcggcaacgt ctcctatgtc gacgtgaccg agcgctttgc
       61 cggccacggc ctctgcggcg aggacccctg gctctacggc atcaagctcg ccgccagcga
      121 cggcctgatc aagggcagct accacccacg ccgcagcggc cagcaggcct acgccgccgc
      181 cttcgcgacc ctcctgcgcc agccggcgat ccgcgccgcc ctcactcttc cctaaagcca
      241 ggtgtgagtc ggaggcgcca ggccctctcc cgaaacactc aagcccggct cccgccccag
      301 ctcgatgaga gggaggggca gcgtcccctc ccaaaacact cgggagcggc gtcagccgct
      361 cgttttggga ggggacgctg cccctcccga acccacctgc tcctcgaagt gcttgctgag
      421 catcgtcacc tgtccctggt agttggagcc caggtcctgg ccgtaaaggc gctctgccgg
      481 ggcactggtg cgctccagcg cgagcttgca gaccggctgg cggtgctcga ccatgaagga
      541 gacgtcgcgc gcccgcacct cgagcgcggc gcggctgccg tggcggccgc cggaggggtc
      601 gtagccgaag ccggggtcga agaagccggc atagtgggtg cgcagctcgc ccgcggtcgg
      661 gtcgtaggcc atcatcacgg cggcgatccg cggcgggatg ctgaccccct cggcggagag
      721 cagcaggtag aagatctccg gctcgagcac gatcctgccg ccggcctccg ggtagaccgg
      781 gtcccagtag tcgcgccagc cgtaggcgcg gatcttcgag aggtcgaccg gcaggctgtt
      841 tttcttcgcc cggtagccga cgacgcggtc ggccgggccg gagacgtcga ggctgaggaa
      901 gaggccgtcg gagaccgcga tctcatcctc gggaaccgga tcggcgtcca ggtagatcag
      961 cggcgactcg gcctgcagct cgcggatccg cgcgtcgccg aggcgcacgt cgccgcgggc
     1021 gaggcgcagc tggttcagcg agagcccggt cttcacctgg atcgcgaagg agcgcgggac
     1081 gatctcaagg tagagcttgc cgtggtagcc ggcgcggacg tcgtcgaagc ggtggtggcg
     1141 gtcggtgatc acgcgggtga agacgtcgag gcgaccggtc gagctcttcg ggttggcctt
     1201 cgcccgcacg tcgtcgggca gcgccagctc ctcgatcagc ggcaccaggt acggacggtc
     1261 gcgctcgagc acggcgccgt cgcgcaggtc gatcttctgg aaggcgagcc cctccatctt
     1321 ttcctcgacg ctcgattcga cgtcgggcag gaaggagcag cgcagggccc aggcgacgtc
     1381 accgaggcgc aggtcgatgc tggccggctg gatcgcctcc gggcggatcc ggtagtcgcc
     1441 ggagctgacc cactcgcggt cgatcgcctg cgcgagcgcc tgcgaaggga aaacgccagc
     1501 gctctgcgcc tcgttcatcc agcggagatt attcgccgcg cctcggccgt gccgaggtag
     1561 ggcgtcaaag cgaattcgag caccgccggg cgcagctcct cgagctgctc gatctcaccg
     1621 gccagggcgc ggcgcccgag cagcgaggcg atccccccga ccaccgcctc gtcggtcgcc
     1681 tgcggcactt cgccgccgcc cggtgccgcc ggggcgccgt cgcggatcag cggcacgaag
     1741 ctctccaggg ccgcctggta gcggtcgtag atgccgcgcc ccgcgcgcag gccctcgacc
     1801 agggtgatcc cggctagcgg cggatcgctg gcgagcaggt cgatcagcga ggagatgccg
     1861 tcgcgcacgc gcgggggcca gtcgtcctcg gggtgcgccg agcaggcggt gaccacacgc
     1921 tcccggatcc gctcgaccgc ggtttcgtag gccgcgtaga agcaatcgtc cttgctctca
     1981 aagtgcttgt agaagtcgct cttggagacg gccgcgcgct gccctatcaa ggaaaccgta
     2041 gttttctgat agccgacctc atggagaact tcggtcagcc cggtgatcag ccgttcgcgc
     2101 tgattctggg cgacaaactc cggggatagt ccatgtcggc cgcgtggcag tcgctccatc
     2161 tatgcataga agtctgccac cgtactgtga gtttcgcttg ccgattactt gggtgaattt
     2221 gttacaccaa ggtacggcga taggacaaag cgtgacaact cgggcaagag agacgcgatc
     2281 ttctgcgttt caccagccct gatccgctgg tggaggatcc aggcaacgcc gccgaccagc
     2341 gactcctcgg tggtctcggg cagcgagatt tcgccttccg gcgagttttc tttgagcagc
     2401 tcgacgaagg cgcgtacggc gtcgtcgtag ctgaccgacg agcgtggcgt ggctgagagc
     2461 gcctcgacca ggcacatccg cgcggccgcc ggctggtcgg cgacgaagcg gagcagggcg
     2521 gcgagccccg attcgatccg ctgctgccag ccgccgcctg tgctgtcgca agcgacttcg
     2581 accgaagtcc tcgcctcggc gaagtaaaag tcgaaggcgc tgaggaaaag gtcctccttg
     2641 ccgtcgaagt tgtcgtaaag cgttttgcgc gctacgcctg cgcggctgac gacgtcggcg
     2701 atcttggtcg cctcgtaacc ctgctcagca ctcagttcag caatcgccgc aatcatgcgg
     2761 cgctgcttat gtaccgcgac gaattctcgt ggaagctcgg gccgagtcgg cttcatcatg
     2821 gtctcccagg gtctgctggg attctctatt gaagaatagc cggggttccc cattcttcgc
     2881 acaagtgacc atcatctctg gtaccgtgaa gaaacttgga tcgtttttgt taggaaccgg
     2941 tgggtttctg ggtgtctttc gatcgaatcc agctgagcca agagattctc gtcgctgcaa
     3001 tcggggagga ctggccgcaa gagcaggttg acgcggccgt cgagctgctc gcaaatagtg
     3061 atggaaagtt ccccgccggg gttcgcaagc tgccgtctga gctggtcaag gcggtccagc
     3121 gcgagcgcct cgtggtcgcg atgctgatgg cggcggccga actcggctat cgcgaaacca
     3181 acgtccagga cgtgatcgac cgcgccggcg tttcccgccc caccttctac gagcacttcg
     3241 cgaacaaaga agactgcttc ctggccgctt tcgacaccag cgcagcacgg ctgcgcaaca
     3301 aggtcgacgc ggccgccgag cgcggcggcg acagctggcg ggaccgcctc cgctacagcc
     3361 tcgaggcgct gatccagttc gcggtcgccg agccgcaaac cgcgcgcacc ctgatcgtcg
     3421 aggcgcgcgc ggccagctcc gacgcggtga tgcgccgcgt gcagctgctc gagtacttcg
     3481 ccgagtgcct cgacgaagac gcgcgccagc ggctgcccga gggcgagtac tcctcggtca
     3541 ccgcggccgg tgtgatcggc ggcgtcgaag cgctgctcta ctcgcgcctg tgcaaggaag
     3601 acgtcgacaa cctcgaggag ctgctgccct cgctgatgta cttcgccgta ctgccctacg
     3661 aggggcacga ggtggcgagc gaagagctcg cgctcgactg acccgcgact agagttgccc
     3721 ggatgagctc gctggccgag atgcgctcgc cgcaggccgc ccgcgccgcg ctcgccttcc
     3781 tcctcttctc cgctctgctg atcgggctga ccgcgacggt cgtcccgcgc accttctacg
     3841 acgacttccc cttcctcgcc agctgggtcg agctgctgcc gccctacaac gaacacctgg
     3901 tcaccgacgt cggcggtctc tacctcggct tcgcggtgat gttcggctgc gccgcctgga
     3961 ccctcgagcg caccctggtc cgcgccgtct ccgtcgcctg gctgctgacc gcaggtatcc
     4021 acctgctctt tcacgccggc cacctcgaca acttctccac cgccgacgcc gtcgccgagc
     4081 tcgccagcct caccctcctc ctcgccccac ccccgatcgc ggtctgggga acctctccgc
     4141 gcccgggcgc ctagaatcgg cgaagcctcg gaggggtgcc ggagtggttg aacggggcag
     4201 tctcgaaaac tgttggcgtc gcaaggcgct cgtgggttcg aatcccaccc cctccgctcg
     4261 cgccgcccga gcagatttgt gcgtctcggc ggcccaggtg tactgaagcg cttcctgggt
     4321 agctcttctc cagacccgag aggaggagcc ggatcatgtc cgagcaaaac tccactacgg
     4381 gggggtcggt tcatcacggg gagatcgact gggaggcggc ggagcggtcc ccggagttca
     4441 gggagctgga gcgtgcgaag cgcagcttcg tgctcccggc gacgatcttc ttccttggct
     4501 ggtacgtcac cttcatcctg ctcgccggct acgccccgag ctttatgggc gagtcgctga
     4561 tcgaggggtt cacggtcggc tacgccctgg ccctgagcca gttcgtgatg gtctgggtgc
     4621 tgaccgcgat gtacctgcgc cgcgccgacc gggacttcga cccgctggcc gagaaggcgg
     4681 tcgcgaaagc gctggagccc cgaaagggtg aggagcgatg agcccggcgg caccgctggc
     4741 ggcggtcaac gtcgaggccg tcgcgatctt cggcgtggtc gtcgcggtca cgctggggat
     4801 caccttctgg gcctcgcggc gcaccacctc ggcgacccag ttctgggccg ccggcaggca
     4861 gatcaccggg cgccagaacg gcctcgcgat cgccggcgac tacatgtcgg cggcctcgtt
     4921 cctcgggatc gccggcctga tcttctactt cggcttcgac ggcttcctct actcggtcgg
     4981 cttcctggtc gccttcctca ccgtgctctt cctgctcagc gagcggatgc ggaacgccgg
     5041 caagttcacg atcgccgacg tgctcgcctt ccggctgaaa gagcgaccgg cccgggtcgc
     5101 cgcggcgctc ggcacgctcg cggtcgtcgc cttctacctg atcgctcaga tggtcggcgc
     5161 cgggatcatc atcgaagggc ttgtcgggat cgacttcaac gtcgccgtga tcgcgaccgg
     5221 cgcgttcatg ctcgtctacg tcgtcttcgg cggaatgctg gcgacgacct gggtgcagat
     5281 cgtcaaggcg atcctgctga tgtcggcgac gatcgtgctc tcgatcttcg tgctggtcaa
     5341 gatcggcggc aacccaatcg acatcttcaa cgacgccaag aaccagtccg aagacccggc
     5401 ggcctacctc gagccgggcc tgcgcttcga caacccgatc gacacgctct cgctcgggct
     5461 cggcctcgtg ctcggcaccg ccggcctgcc gcacatcctg atgcgcttct tcaccgtgcc
     5521 cgacgccaag gccgcccgca actcgatcgg ctgggcggtc ggggtgatcg gcgtcttcta
     5581 cgtgatgacg accttccttg ggttcggcgc gcgggcgctg ctcggcgaag ccgggaccac
     5641 cggggcggtc gacgaggccg gcaacctggc ggcgccgctg ctcgcccagg aggtcggcgg
     5701 cggcgccggc tccttcggcg gcgacgcctt cctcgcggtc atctccgcgg tcgccttcgc
     5761 gacgatcctc gcggtcgtcg ccgggctggt gatcgcggcc tcgggggcgg tcgcccacga
     5821 cgtctggtcg aacgtggtcc gccggggccg cgactccgag cacgaggagg tctacgtcgc
     5881 ccggatcgcc gccctctcga tcggcgcgat cgcgatcgca atcgcggtga tcggcggcag
     5941 cgacctcaac gtctcctaca tggtcggcct cgccttcgcg gtcgccgcca gcgccaactt
     6001 cccggccctg ctgctggcct tgacctggcg ccgcttcaac accaccggtg cggtgtcggg
     6061 cgtggtggtc gggaccgtga gcgcgatctt cctcgtcgtg accggccccg atgtggccag
     6121 cgacccgctc gtcgacctga cccagccggg catcatctcg atcccgctcg gcttcgccgc
     6181 ctgcatcatc ggcacgctgc tcggccgcga cgagacccag gccgagcgct ccttcgacga
     6241 gctgcacgtg cgcgccgaaa ccggcctggg cgccgaggtc ggcgaagccg cgcggccaac
     6301 caggcagcga ccgcgacagc ccgtgggggc agcctccgcg tctcccgact agcgtttttt
     6361 cgggaccagc ttggcggcga gcgtgccgag ggtcgtctcg ccgccgggga ccttgccggt
     6421 gacggcgatc ttggcctgta gctgaccggc ggcgaggagc tttttcaggc ccgctttcga
     6481 gagcttgccg gtcaggctga cggttttgcc gggggcgact ttgagctgcg aggagccgag
     6541 cgcggctttc ggcttcttcg cccctttcac cttgaccgcg acggtgcagg gtgcggtgct
     6601 cgcggccgga caggtgagct t
//
LOCUS       DASRIG010000087         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1604648, whole genome shotgun sequence.
ACCESSION   DASRIG010000087 DASRIG010000000
VERSION     DASRIG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604648"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFB52_02355"
     CDS             complement(<1..754)
                     /locus_tag="VFB52_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO05199.1"
                     /translation="MNRVLITGGAGFVGSNLAVSLAGRHPDWEIVALDNLYRRGSELN
                     LPRLAEAGVEFVRGDVRELETLKALPAFDALIECSAEPSVMSGVNGDTGYLVHTNLTG
                     AYNCLELARRDGAFLVFLSTSRVYPVAPQLQINLEEAETRFEIAPEQDLSGVSPAGIS
                     EAFPLDGERTLYGATKLAAELLIEEFRGSLGVPAVIDRCGVIAGPWQMGKVDQGVFTH
                     WMLAHQFENPLTYIGFGGEGKQVRDLLHVEDLV"
     gene            887..1765
                     /locus_tag="VFB52_02360"
     CDS             887..1765
                     /locus_tag="VFB52_02360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO05200.1"
                     /translation="MGEIDPVKVYEESGRDHRALIESLLPDDWSWEGRRTLDFGCGVG
                     RIMRQFAPEADSAEFWGCDLDRPSIDWLNRNLEPPFHFFVSDEEPDLPQEDGFFDLIY
                     AFSVYTHFTDNWAAWLLEHHRVLKDDGLLLVTFLGEGMTDTLIGEKWDEDRIGMNPLL
                     YGNSWELGGPIAFNSEWWIRAHWGRAFEILELRPHDGSDPPYGHGIALMRKKPVSLTV
                     EDLKALEPDEPREILAIQHNVEQLRDDTLRLREALAQQEQRLRGEYERSASWRATAPA
                     RAAKRVLQEQRSRRKR"
     gene            1810..3378
                     /locus_tag="VFB52_02365"
     CDS             1810..3378
                     /locus_tag="VFB52_02365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO05201.1"
                     /translation="MGALDPPPGGATLRRGAIAFGGWTHFRSGPAARVELRLGDQPLG
                     RARLGLRRTDACDALGLPVGAATGFELLCDLSTWPGGEGELELTALATGLRGERLELG
                     PHHVRVAAPEQARMVRPTPPLHQRVGPPSGRGLRTLVVTHRLDRGGAQLYLQELLAEL
                     VRGGTVDPVVVSAEDGPLRAELEALAVEVHVTGTPPFESLDAHRDRVGELAAWAGEGG
                     FEVAFVNTATGPAYFGVDLAAALGIPVLWSIHESYPPAVLWSGMHPELLRHAEAALTR
                     AERTVFAAESTRRLLAPHIAAERSRTIPYGLEFGALEARRAGFDRAAARRAAGISAEA
                     DVLLCVGTVEPRKAQLPLAQAFELVAARHPRAELIYVGARPGEPDTDFLTEYAAASPA
                     ARRIRVVSMTDDVHSWFGLADLFVCAADVESLPRTVVEAQAWELPVLATAIFGLPELV
                     VDGETGWLCEPLDLEALAAGLDRALASSPEQRRRLGRNARAQVLARHDLPTYATEIAA
                     LIAELAGRPARALA"
     gene            complement(3375..>3951)
                     /locus_tag="VFB52_02370"
     CDS             complement(3375..>3951)
                     /locus_tag="VFB52_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HZO05202.1"
                     /translation="GRIADRLAVEHPAVEVLHRTRKEGLGPAYIAGFRHALAAGAGLI
                     LEMDSDFSHDPADLGRLLAAAEDADLVIGSRYVRGGAIGDWSALRRGISRGGSAYARV
                     VLGVGVRDLTGGFKCFRREVLEAIDLDAVDAKGYAFQVEMTYRAIRLGFRVAEIPITF
                     SERQHGDSKMNGSIVAEAAWRLPLLRWRERR"
BASE COUNT          538 a         1481 c         1398 g          534 t
ORIGIN      
        1 cgaccaggtc ctcgacgtgg agcaggtcgc ggacctgctt gccctcgcct ccgaagccga
       61 tgtaggtaag cgggttctcg aactggtggg ccagcatcca gtgggtgaat accccctgat
      121 cgacctttcc catctgccag ggcccggcaa tcaccccgca gcggtcgatc acggcgggca
      181 cgccgaggct gccgcggaac tcctcgatca gcagctcggc ggcgagcttg gtggcgccgt
      241 agagcgtgcg ctcgccgtcc agggggaagg cctcggagat ccccgccggc gagactcccg
      301 agaggtcctg ctcgggggcg atctcgaacc gggtctcggc ctcttcgagg ttgatctgca
      361 gctgcggcgc gaccgggtag acgcggctgg tcgagaggaa gacgaggaag gcgccgtcgc
      421 gccgggcgag ctcaaggcag ttgtaggcgc cggtcaggtt ggtgtggacg aggtagccgg
      481 tgtcgccgtt gacgccgctc atcaccgaag gctcggcgga gcactcgatc agcgcgtcga
      541 aagcgggcag cgccttcagc gtctcgagct cgcgcacgtc tccgcgcacg aactcgaccc
      601 cggcctcggc caaccgcggc aggttcagtt cggagccgcg ccggtagagg ttgtcgagcg
      661 cgacgatctc ccagtccggg tgccgcccgg ccagggaaac cgccaggttc gacccgacga
      721 aaccggcgcc accggtgatc aggacgcgat tcacggcgct gaagctatcg gacccatccg
      781 gctgcttgga accgcgccgc tccggtaggt tgcgctcgtg gctgtatctg gctctgcctc
      841 cccgcttccc ctcccgccgc cgaacctcct gcagaggatc ggcccgatgg gcgaaatcga
      901 cccggtcaag gtctacgaag agtccggtcg cgaccaccgc gcgctgatcg agtcgctgct
      961 gccggacgac tggtcctggg agggccggcg cacgctcgac ttcggctgcg gggtcggccg
     1021 gatcatgcgc cagttcgccc ccgaggcgga ctcggccgag ttctggggct gcgacctcga
     1081 ccggccgagc atcgactggc tgaaccgcaa cctcgaaccg cccttccact tcttcgtctc
     1141 cgacgaagag cccgacctcc cccaggagga cggcttcttc gacctgatct acgccttcag
     1201 cgtctacacc cacttcaccg acaactgggc cgcctggctg ctcgaacacc accgggtgct
     1261 caaagacgac ggcctgctgc tggtcacctt cctcggcgag gggatgaccg acacgctgat
     1321 cggcgagaag tgggacgaag accggatcgg gatgaacccg ctgctctacg gcaactcctg
     1381 ggagctcggc gggccgatcg ccttcaactc ggagtggtgg atccgcgccc actggggccg
     1441 cgccttcgag atcctcgagc tgcgcccgca cgacggcagc gacccgccct acgggcacgg
     1501 gattgcgctg atgcggaaaa agccggtgtc cctgacggtc gaggacctaa aggcgctgga
     1561 gcccgacgag ccgcgcgaga tcctcgcgat ccagcacaac gtcgagcagc tgcgcgacga
     1621 caccctgcgc ctgcgcgagg cgctggctca gcaggagcaa cgactgcgcg gcgagtacga
     1681 gcggtcggcc tcctggcggg cgacggcacc ggcgcgcgcg gcaaaacggg tcctgcagga
     1741 gcagcgatcc cggcgcaaac gctgaagcgg ctcgcggccc ggctcggcgg ccggggcccg
     1801 gcggaggcgg tgggcgcgct cgacccgccg cccggcggcg cgaccctgcg ccgcggcgcg
     1861 atcgccttcg gcggctggac ccacttccgc tccgggccgg cggcgcgggt cgagctgcgg
     1921 ctcggcgatc agccgctggg acgggcgcgg ctcgggctgc gccggaccga cgcctgcgac
     1981 gcgctcgggc tgccggtcgg agcggcgacc gggttcgagc tgctctgcga cctctcgacc
     2041 tggcccggcg gcgagggtga gctggagctg accgcgctgg ccaccgggct gcgcggcgag
     2101 cgcctcgagc tgggaccgca ccacgttcgc gtcgccgccc ctgagcaagc ccggatggtt
     2161 cgtccgacgc cgcccctgca ccagcgggtc ggcccgccga gcgggcgcgg cctgcgcacc
     2221 ctcgtcgtca cccaccggct cgaccgcggc ggcgcccagc tctacctgca ggagctgctc
     2281 gccgagctgg tccgtggcgg caccgtcgac ccggtcgtag tcagcgccga agacgggccg
     2341 ctgcgagccg agctggaagc gctcgcggtc gaggtccacg tgaccggcac gccgccgttc
     2401 gagagtctcg acgcgcaccg cgaccgggtc ggcgagctgg ccgcctgggc gggcgagggc
     2461 ggcttcgagg tcgccttcgt caacaccgcc accgggcccg cctacttcgg cgtcgacctt
     2521 gccgccgcgc tcggcatccc agtgctctgg tcgatccacg agagctaccc gcccgcggtg
     2581 ctctggtccg gcatgcaccc ggagctgctg cgccacgccg aagcggcgct gacgcgggcc
     2641 gagcggaccg tgttcgcggc cgagtcgacc cgccgcctgc tcgcccctca catcgccgcc
     2701 gagcggtcgc ggacgatccc ctacgggctc gagttcgggg cgctggaggc tcgccgggcg
     2761 ggcttcgacc gcgccgccgc tcgccgcgcc gccggcatct ccgccgaggc cgacgtgctg
     2821 ctctgcgtcg gcacggtcga gccgcgcaag gcgcagctgc cgctcgccca ggctttcgag
     2881 ctggtcgccg cgcgccatcc acgggccgag ctcatctacg tcggcgcccg ccccggcgag
     2941 cccgacaccg acttcctcac cgagtacgcc gccgccagcc ccgccgcccg ccgcatccgc
     3001 gtggtctcga tgaccgacga cgtccactcc tggttcggcc tcgccgacct cttcgtctgc
     3061 gccgccgacg tcgagtccct gccgcgcacg gtggtcgagg cccaggcctg ggagctgccc
     3121 gtactcgcaa ccgcgatctt cggcctaccc gagctggtcg tcgacggcga gaccggctgg
     3181 ctctgcgagc ccctcgacct cgaggcgctc gccgccggcc tcgaccgggc cctcgcctcc
     3241 agccccgagc agcgccgccg cctcggccgc aacgcccgcg cgcaggtgct cgcccgccac
     3301 gacctcccca cctacgccac cgagatcgcc gcgctgatcg ccgagctcgc cggccgcccc
     3361 gcccgggcgc tcgcctagcg ccgctcgcgc cagcgcagca gcgggaggcg ccaggcggcc
     3421 tcggcgacga tcgagccgtt catcttcgag tcgccgtgct ggcgctcgct gaaggtgatc
     3481 gggatctcgg cgacgcggaa gccgaggcgg atcgcgcggt aggtcatctc gacctggaag
     3541 gcgtagccct tggcgtcgac cgcgtcgagg tcgatcgcct cgaggacctc gcggcggaag
     3601 cacttgaagc cgccggtaag gtcgcgcacg ccgaccccga gcacgacccg ggcgtaggcg
     3661 ctgccgccgc ggctgatccc gcgccgcagg gcgctccagt cgccgatcgc gccgccgcgc
     3721 acgtagcgcg agccgatcac caggtcggcg tcctcggcgg ccgcgagcag gcggccgagg
     3781 tcggcggggt cgtgggagaa gtcggagtcc atctccagga tcaggccggc accggcggcg
     3841 agcgcgtggc ggaagccggc gatgtaggcg ggtccgaggc cttccttgcg ggtgcggtgg
     3901 aggacctcga ccgcggggtg ctcgacggcc agccgatcgg caattcggcc a
//
LOCUS       DASRIG010000088         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_301346, whole genome shotgun sequence.
ACCESSION   DASRIG010000088 DASRIG010000000
VERSION     DASRIG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301346"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFB52_02375"
     CDS             <1..387
                     /locus_tag="VFB52_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05203.1"
                     /translation="FAGISAAIELADADLPPSELEQVVGDDIYHRRFVLGGEEPVPLP
                     ESPIAVAVTRDGAPLASTDDAEAATGSLPELIAHVAAYLAEFGCDLAAGDVVISGSTV
                     PLIDVAPGQRVRSEVAGVGAVEVALT"
     gene            384..1445
                     /locus_tag="VFB52_02380"
     CDS             384..1445
                     /locus_tag="VFB52_02380"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZO05204.1"
                     /translation="MSASPKVAVLGAGANGASIGADLTRAGVDVVLIDQWPEHVEAMR
                     ANGVRIEMPEETLTVPVRAHHLCDVATFEERFDIVLVVMKAYDAEWACRLIAPYVKPD
                     GLVAGVQNGMTAATIADAVGVERTVGCVIEVSSMMTVPGVVERHSPPSRSWFAVGSFD
                     AAGTRRAEEVAALLRHAGKVEAVDDILAAKWMKLVSNCTTLVTTAILGLPMLAALNEP
                     GMRELMLRAGQEALDAGLARGHGIPPIFGLSAADVEDPDRVVEVLLDTLYAGFVLEHT
                     TTTILHDWLKGRHSEVDDINGLALSEARRHGLDAPVNAAIVELAHRIERGELEPRPEN
                     LALLHELAAADVIPGERFR"
     gene            complement(1590..>2117)
                     /locus_tag="VFB52_02385"
     CDS             complement(1590..>2117)
                     /locus_tag="VFB52_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO05205.1"
                     /translation="RLVSNVEKLKVGDPLDPDTDVGPLITPGDRDRVKEWIDEAVASG
                     AELLAGGELVDEGRCLAPTLLAHPSREAKVWCEEIFGPVATIDTFTDFDEALAMANDS
                     KFGLQAGVFTRDIAKGLKAAQTLEFGGVLINEVPTFRADQMPYGGVKDSGNTREGPAF
                     AVYELTEERFVTLQG"
BASE COUNT          308 a          728 c          750 g          331 t
ORIGIN      
        1 ttcgccggga tctcggcggc gatcgagctg gctgacgccg acctgccgcc gagcgagctg
       61 gagcaggtgg tgggtgacga catctaccac cggcggttcg tgctgggtgg ggaggagccg
      121 gtgccgctgc cggagagccc gatcgcggtt gcggtgacgc gcgacggcgc gccgctggcc
      181 agcaccgacg acgccgaggc ggcgaccggc agcctgcccg agctgatcgc ccacgtcgcc
      241 gcctacctcg ccgagttcgg ctgcgacctc gccgccggcg acgtcgtgat cagcggttcg
      301 accgtgccgc tgatcgacgt cgcgccgggc cagcgcgtcc gctccgaggt cgccggcgtc
      361 ggcgcggtcg aggtcgcgct gacgtgagcg cctcgccgaa ggtcgcggtc cttggtgccg
      421 gcgccaacgg tgcctcgatc ggcgccgacc tgacccgcgc cggggtcgac gtcgtcctga
      481 tcgaccagtg gcccgagcac gtcgaggcga tgcgcgccaa cggcgtgcgg atcgagatgc
      541 ccgaggagac gctgaccgtg ccggtccgcg cccaccacct ctgcgacgtc gccaccttcg
      601 aggagcgctt cgacatcgtc ctcgtcgtga tgaaggccta cgacgccgag tgggcctgcc
      661 gcctgatcgc gccctacgtg aagcccgacg gcctcgtcgc cggggtccag aacgggatga
      721 ccgcggcgac gatcgccgac gcggtcgggg tcgagcgcac ggtcggctgt gtgatcgagg
      781 tctcctcgat gatgaccgtg cccggtgtgg tcgagcgcca ctcgccgccc tcgcgctctt
      841 ggttcgcggt cggcagcttc gacgccgccg gcacccgccg cgccgaggag gtcgcggcgc
      901 tgctccgcca cgccggcaag gtcgaagccg tcgacgacat cctcgccgcc aagtggatga
      961 agttggtcag caactgcacg actctggtca ccaccgcgat cctcggcctg ccgatgctgg
     1021 cggcgctgaa cgaaccgggc atgcgcgagt tgatgctgcg cgccggccag gaggcgctcg
     1081 acgccggcct cgcccgcggc cacggtatcc ccccgatctt cggcctctca gcggccgacg
     1141 tcgaagaccc cgaccgcgtg gtcgaggtcc tgctcgacac cctctacgcg ggcttcgtgc
     1201 tcgagcacac caccacgacg atcctgcacg actggctgaa gggacggcac agcgaggtcg
     1261 acgacatcaa cggcctggcg ctgagcgagg ctcggcggca cggccttgac gccccggtga
     1321 acgcggcgat cgtcgagctc gcccaccgga tcgagcgcgg cgagctcgag ccgcggccgg
     1381 agaatttggc gctgctgcat gagcttgcgg ccgcggacgt gattccgggg gagcggtttc
     1441 ggtaagcgac gaaccgggcc accccgcaca ccgccaaggg ctggtaggtg tgcgtggtgg
     1501 cccggttgaa gtggagagcg gtggggtgat ttagcgcttg tcctctaatg ctcgctatag
     1561 cgagttcgat aggacaagcg cggaagggct tagccctgca acgtcacaaa ccgctcctcg
     1621 gtcagctcat agaccgcaaa cgccgggccc tccctggtgt tcccggagtc cttgacccct
     1681 ccgtagggca tctggtcagc gcggaaggtc gggacctcgt tgatcagcac gccgccgaac
     1741 tccagggtct gggcggcctt cagtcccttg gcgatgtcgc gggtgaagac gcccgcctgc
     1801 aggccgaact tcgagtcgtt ggccatcgcc agtgcttcgt cgaagtcggt gaaggtgtcg
     1861 atggtggcca cgggtccgaa gatctcctcg caccagacct tggcctctct ggagggatgg
     1921 gcgagcaggg tgggcgcaag gcagcggccc tcgtcgacca gctctcctcc ggccagcagc
     1981 tctgcgcccg aggcaactgc ctcatcgatc cactccttga cgcggtcccg gtcgccgggg
     2041 gtgatcaggg gaccgacgtc ggtatcgggg tcgagggggt ctccgacctt gagcttctcc
     2101 acgttggaga caagacg
//
LOCUS       DASRIG010000089        11579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2915677, whole genome shotgun sequence.
ACCESSION   DASRIG010000089 DASRIG010000000
VERSION     DASRIG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915677"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFB52_02390"
     CDS             <1..656
                     /locus_tag="VFB52_02390"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HZO05206.1"
                     /translation="VSGGELWRVGHRGDPLAFTPVELCAWNHRFDDVHRRFRTVYCAE
                     RPETSLREVLADLRPNLEARLTFAEAFGAAMLDDLPTELVTARWREEHMLAPAKLVRS
                     GELIDLTEPAVRTELEQRHAELLIEHGLQHLDLHEITARRRALTQTIASDLFDNYGAA
                     ALRFPSRLDGRPALVAFEGRAQLVEAGDPIALTDPAPPLLNQVCEEWSLQLERVDPL"
     gene            complement(874..1170)
                     /locus_tag="VFB52_02395"
     CDS             complement(874..1170)
                     /locus_tag="VFB52_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05207.1"
                     /translation="MLISVTLVGGLSVPASVAAPIRECGDMPSRFAYNITTRKAACWK
                     AREVIRRWGNTLAQEGGDGHVGAYYCNYKNIGYEAGDIRCTASRGRVVHWQTGV"
     gene            complement(1250..1600)
                     /locus_tag="VFB52_02400"
     CDS             complement(1250..1600)
                     /locus_tag="VFB52_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05208.1"
                     /translation="MAKALMVVGSNPTDPAQEAAYNEWYTETHLDDVLKVAGFNAARR
                     YNLSPVRPMVGTEPSPFGYLAVYEVESDDLAATARELQAALDSGAIPLSETFDLSAFS
                     VDFYELLPDSERSA"
     gene            complement(1620..2057)
                     /locus_tag="VFB52_02405"
     CDS             complement(1620..2057)
                     /locus_tag="VFB52_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO05209.1"
                     /translation="MRRPADGVTASWLVPALVYVVILGALGVTSKLALRTLSWHDLLL
                     WTGIAYLGFALVLLALGEASFRVDGNFGWAVLSALIAPTALILLYFALGHGESSQIFP
                     VTAAYPAVTVALAAIVLSEPLTVAKVGGMVLVLSGVVLISISN"
     gene            complement(2033..3184)
                     /locus_tag="VFB52_02410"
     CDS             complement(2033..3184)
                     /locus_tag="VFB52_02410"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZO05210.1"
                     /translation="MNPAQHPSLRTQVTELLGVTYPLLAFSHSKEVVVAVSRAGGLGV
                     LGAAQCSVEELEQKLAWLEQELGDVPFGVDLLLPGRYEKAEEGGLQREELIAQVPSAH
                     REFVADLMDQYAVPELEEGYEFGDIVDGGGVPVTGNHTYTAKDTQPLIDVCLNSKIAL
                     FANALGVAPREVIDSFHDHGILVAGLAGTARHGEKHKQAGTDIVIAQGYEAGGHTGEI
                     GSMVLIPDVVDAVEGLPVLAAGGIASGRQMAAAMVLGASGVWTGSVWLTTEESEVIAP
                     VKEKFVDAVAAETVRSRSCTGRPARQLRSAWTDAWEGEIEPLGMPHQGILYAEPQVRI
                     ARGAEADAGRSRELATYFVGQVVGRLDRIRPASEVVEEMVTECAALLTA"
     gene            complement(3184..4203)
                     /locus_tag="VFB52_02415"
     CDS             complement(3184..4203)
                     /locus_tag="VFB52_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO05211.1"
                     /translation="MSAARRGVAVFGTTLEAMSAAGEAVDKAGFDAVWTSELYNRSAT
                     VTLAAITPRTKQAAIGSGIIYGVGRSPLMLAAEARDLDELSGGRFILGIGNGTRRMIS
                     DWHGLDGDAPAVRMEELVPLVRQVWNLAEAPIDHEGRFYRLKLRALDESAGVPERQIP
                     IYTAGVGPRMIESAGRVGDGLLAHTMCAPRYLEDVARPAVERGAAKTGRDAGEVAIAT
                     YALACAAPDAELARREAAGMIAFYGTVKSYATVFEHCGFGAEATAIQEAFAKRDLKAM
                     VGAVSEPMIDEFANAGTPEEVDAKLRRYDGVVDEVVLSVPSFQVSDERVAENLSLLTE
                     RCAPK"
     gene            complement(4200..5123)
                     /locus_tag="VFB52_02420"
     CDS             complement(4200..5123)
                     /locus_tag="VFB52_02420"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO05212.1"
                     /translation="MSDSPKSRSFTTIEFEVSDAIATITLNRPEQLNALDPTMERELL
                     QVWDLVDEDDDVRAVVVTGRGRAFCAGFDLTEAGFDVYRAAELRGTEELGPGDIPRDS
                     GGLIALRIFRCLKPVVAAINGAGVGFGATFPLPMDVRLASEKARFGFVFSRRGMCMDA
                     ASSWFLPRAVGLSRALEWSISGRIFDAEEALEGGLVRSLHPPEELLEAAYEVARGMIE
                     ASAPVSVALNRQLLWQMAGAAHPMDAHRQESIYIAERAMGADAKEGADSFMEKRPPSF
                     PLTVSADLPAAVPPYEEPPFDRRRNSAGAPR"
     gene            5244..5600
                     /locus_tag="VFB52_02425"
     CDS             5244..5600
                     /locus_tag="VFB52_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HZO05213.1"
                     /translation="MPTDALLDDAGALAAAVAGPPIGDSIALRRDEDGTVVTSCGECG
                     YAFGPADRDPKLGAVMRERSIADLSPLNAEGMTERLVARHFHCPQCALLLAVNVQQVG
                     DPIMLEWRLDVDGKGA"
     gene            5604..9632
                     /locus_tag="VFB52_02430"
     CDS             5604..9632
                     /locus_tag="VFB52_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO05214.1"
                     /translation="MYDIAVDIGGTFTDCVIHDAERELVMAAKAPSRRDNLGGGVLDS
                     VEAAAERLGVPLEQVLANTRRFIHGSTVATNAMIERDGARTAYVTTAGHEDTLAIGKI
                     FQKRAGLSEREISHLNRLQMADPPLVPRELVFGVQERMDYRGRVVVALSDAEIAATIE
                     RVRESGVESVAVCLLWSFIEPEHERRLTEAMRAALPDVYVSASVDVSPVLGEYERGVT
                     TALNAYLGPPISRYLGGLQELLSENGLSAQVLLMTAGGGVVSIDSARDKPVTLLDSGP
                     VGGTLGSRFVGSGYDEENIICTDVGGTSFDVSLVIGEEFQLEHEPVIAQYVFKIPKVA
                     VKSIGAGGGSIAWVDGLGVLKVGPQSAGARPGPACYGRGGELPTVTDANLILGYLNPD
                     NFLGGAMTLDREAAVRAFEPLAEQLGLSVEEAAAGVHKIMNSHMADLLRGSTIERGHD
                     PRDFELFAYGGAAATHAVGYSAEAGVRSIRIPADATAFSALGMLTADLTHSFERGRPV
                     STPFSDEDFETMASVFAELGEQATEQLRLDGEDPEEATMRRSLLMRFRAQVHEIEIDV
                     PAEISLDAAGIDALIERFTERYEQTYGEGSAYPDAGVEITTFRLTATIPTSLDAIPGL
                     GERSGSASEAKTGTRDAYFEGAGFGPVDVYDGERLAPGNRVEGPAVIERYGDTVLIPP
                     GLAGEMDGRGSITVEIGEAAVAGSDRDRVSGGRLDPITYEVIRNRLWAINDEQAQTAA
                     RKSGSQFIYEAFDFNAGLLDGDGNGLFAGVYVLFHTTGLDMTVKEIMRRFPDISDGDV
                     FITNDPWVGAIHFNDFVTVTPIFADGEIVSWAALVMHYQDVGGPVPGSFVVGSHNVFE
                     ECPIITPLKLMDKGRYCQEVEDLLLRNTRTPMMNGLNLRATVASQITTKRRVLEIVER
                     YGVDALKAATAQVQEEVEETVRGRLAEIPDGEWYDHVYLDHDGHSYELYEVKLRLIKR
                     DGKLIFDFTGTSEQAPGMINGTISGLRGGVMTAVLVMLGYEMPWSTGGLRNIIEIVSE
                     PGTINNCEYPAPCSGGSVCAEEATENVAGTVIGKMLGASAALREESMTVWYPYFNIVI
                     LAGIDQYGEPLASLMFDNSAGGGGARLWGDGVDCGGYFESVSCVCPNVESNEKIFPVL
                     ELYRRRAAGSYGHGQRRGGTGIEFATISYDVDDDLEVIVTNHGAAQPGAPGLYGGFPC
                     ALNTNVILRDADTRERLGGAGAISGEAEVSARGREVLEAKARTTLSTADVLITRNEGG
                     PGYGDPLRRDPAAVAADVRTALCTCEDAEEIYGVALDGDGFDADATAARREQLKAERL
                     ADSVPVGEAVARYGVEGVGAAR"
     gene            complement(9599..10513)
                     /locus_tag="VFB52_02435"
     CDS             complement(9599..10513)
                     /locus_tag="VFB52_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO05215.1"
                     /translation="MPSPEMEATARQFAGPFPLDPAGGRTAYDALGSVLPPDPETRWE
                     ATEVGGIGGPHSEWQWWDGVGEERVVLYMHGGGYVIGSPPSWRQFGGRLSREANVRLL
                     NFDYRLAPEHPFPAAVEDAIAAYRWLLSQGWDPERIALGGDSAGGALGITLMVALRDA
                     GDPLPACHLSLCPWADLTNGAESLDDPSVEDPNPELRPLAEAMAENYLQGQDPEHPHA
                     SPLFADLAGLSPVQIEVSGRDGIRSDALRLGEALREAGVEVSLNDAEGTIHGYHMFAP
                     ETPEASEGIARLSAFLETHLAAPTPSTP"
     gene            10601..>11579
                     /locus_tag="VFB52_02440"
     CDS             10601..>11579
                     /locus_tag="VFB52_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZO05216.1"
                     /translation="MSSRAFVIGVGMTKFEKPGRREWDYPDMVEEAGTKALADAGVAY
                     DLIDQVYAGYCYGDSSYGQRGVYKLGLTGVPLVNVNNNCSTGSSALFLARQAVSGGHA
                     ECALAIGFEKMERGSLGMKYDDRAGALDVHIDAMKDVREWEGSPFAPQMFGNAGREHM
                     DLYGSTADDYAWIGWKNHKHSVNNPYAQFQDEYSLEDVKAAKMIHEPLTKLQCSPTSD
                     GSAAALVASERFVEEHGLWDQAIEIAGQAMVTDLSSTFDTGSCIKIVGGDMSKEAARI
                     AYEQAGVGPEDVDVCELHDCFSTNELITYEALGFAAEGEGQNLVRAEATT"
BASE COUNT         1738 a         3965 c         4202 g         1674 t
ORIGIN      
        1 gggtgagcgg gggagagctt tggcgggtcg ggcatcgagg cgaccccctg gccttcacgc
       61 cggttgagct ctgcgcctgg aaccaccgct tcgacgacgt ccaccggcgc ttccggaccg
      121 tctactgcgc cgagcgcccc gagacctcgc tgcgtgaggt gctcgcggat ctgcgcccga
      181 acctggaggc gcggctgacc ttcgccgagg ccttcggcgc ggcaatgctt gacgacctcc
      241 cgaccgagct ggtcaccgct cgctggcgcg aggagcacat gcttgccccg gcgaagctgg
      301 tgaggagcgg cgagctgatc gatctcaccg agccggcggt ccggaccgag ctcgagcagc
      361 gccacgccga gctgctgatc gagcatggcc tccagcacct cgacctgcac gagatcaccg
      421 cacggcgccg agcgctgacc cagacgatcg cctccgacct cttcgacaac tacggggccg
      481 ccgccctgcg cttcccctcg cgccttgacg gacggccggc cctcgtcgct ttcgaggggc
      541 gggcgcagct ggtcgaggcc ggcgacccga tcgcgttgac cgacccagcg ccgccgctgc
      601 tgaatcaggt ttgcgaagag tggagcctgc agctcgagcg cgtcgacccg ctgtagcggt
      661 gcggccgtca agaccaactg tggcaagccc gcgcgctaga ttcgcgtcat ggtggaaccg
      721 gatgagtacg aggaagctca tggatcgcac cgcgtcgtga cgattcggat cgacgagttg
      781 ctgatccgct actccgggtg cattgactcc ggtgggagcc tgcgcgagcg agtggagggc
      841 ctgcgtaggg agtggcgacc cgcggcgagc ggcctagacg ccggtctgcc aatgaacgac
      901 ccttccgcgt gaagccgtgc agcggatgtc gccggcctca tagccgatgt tcttgtagtt
      961 gcagtagtag gcgccgacat gcccatcccc gccttcttgg gcaagggtgt tgccccagcg
     1021 gcgaatcact tcccttgcct tccagcaagc ggccttgcgg gtcgtgatgt tgtaggcgaa
     1081 gcgactgggc atatcgccgc actcgcgaat cggcgccgcg acgctggcgg ggaccgagag
     1141 ccctcccacc agcgtgacgc tgatcagcaa gcctgcgaga aatgcaagcg tcttcgaccg
     1201 tccccccacg ccgcggcatc ttggtcgagc cgcgggtcgg ttgtcaagct caggcgctgc
     1261 gctcggagtc ggggaggagc tcgtagaagt cgacgctgaa ggcgctgagg tcgaaggtct
     1321 ccgagagcgg gatcgcgccg gagtcgaggg cggcttgcag ctcgcgggcg gtggcggcga
     1381 ggtcgtcgga ctcgacctcg tagacggcga ggtagccgaa gggggagggt tcggtgccga
     1441 ccatcgggcg gacggggctg aggttgtagc ggcgggcggc gttgaagccg gcgaccttga
     1501 gtacgtcgtc gaggtgggtc tcggtgtacc actcgttgta ggcggcctcc tgggccgggt
     1561 cggtggggtt gctgccgacg accatcagtg cctttgccat ctgggtctcc ttggttggtc
     1621 tagttcgaga tcgagatcag gacgacgccg ctgagcacga gcaccatgcc gccgaccttg
     1681 gcgacggtca ggggctcgga gaggacgatc gcggcgaggg cgacggtcac ggccgggtag
     1741 gcggcggtga ccgggaagat ctggctggat tcgccgtggc cgagcgcgaa gtagaggagg
     1801 atcagcgcgg tcggggcgat cagggccgag agcaccgccc agccgaagtt gccgtcgacg
     1861 cggaagctgg cctcgccgag cgccagcagg accagcgcga agccgaggta ggcgatcccg
     1921 gtccagagca gcaggtcgtg ccagctgagg gtgcgcaggg cgagttttga ggtcaccccg
     1981 agggcgccga ggatcaccac atagacgagt gccgggacca gccaggaggc ggtcacgccg
     2041 tcagcagggc ggcgcattcc gtgaccatct cctcgaccac ctcggaggcg gggcgaatgc
     2101 ggtcgaggcg gccgacgacc tggccgacga agtaggtggc aagctcccgc gagcggccgg
     2161 cgtcggcttc ggcgccgcgc gcgatccgca cctgcggctc ggcgtagagg atgccctggt
     2221 ggggcatccc gagcggctcg atctcgccct cccaggcgtc ggtccaggcg gagcggagct
     2281 ggcgggcggg gcggccggtg caggagcgcg agcgcacggt ctccgcggcg accgcatcga
     2341 cgaacttctc cttgaccggc gcgatcacct ccgactcctc ggtggtcagc cagaccgagc
     2401 cggtccagac gccgctggcg ccgagcacca tggccgccgc catctggcgg ccgctggcga
     2461 tcccgccggc ggcgaggacg ggcagccctt cgaccgcgtc gacgacgtcg gggatcagca
     2521 ccatgctgcc gatctcaccg gtgtggccgc cggcctcata gccctgggcg atcacgatgt
     2581 cggtgcccgc ctgcttgtgt ttttcgccgt ggcgggcggt gccggcgagg ccggcgacga
     2641 ggatgccgtg gtcgtggaag gagtcgatca cctcgcgcgg ggcgaccccg agcgcgttgg
     2701 cgaagagcgc gatcttcgag ttcaggcaga catcaatgag gggctgggtg tccttggcgg
     2761 tgtaggtgtg gttgccggtg accgggacgc cgccgccgtc gacgatgtcg ccgaactcgt
     2821 agccctcctc cagctccggc accgcgtact ggtccatcag gtcggcgacg aactcgcggt
     2881 gggcgctcgg cacctgggcg atcagctcct cgcgctgcag cccgccctcc tcggccttct
     2941 cgtatctgcc cggcaggaga agatccaccc cgaaggggac gtcgccgagc tcctgctcca
     3001 gccaggcgag cttctgctcc agctcctcga cgctgcactg ggcggcaccg agcacgccga
     3061 ggccgccggc gcgggagacc gcgacgacca cctctttgct gtgggagaag gcaagcaggg
     3121 ggtacgtgac gccgagcagt tcggtgacct gggtgcgcag ggagggatgt tgggccgggt
     3181 tcatcacttc ggggcgcacc tttcggtcag cagggagagg ttctcggcga cgcgctcgtc
     3241 ggagacctgg aagctcggca ccgagagcac gacttcgtcg acgacgccgt cgtagcggcg
     3301 cagcttcgcg tcgacttcct ccggggtccc ggcgttggcg aattcgtcga tcatcggctc
     3361 ggagacggcg ccgaccatcg ccttcagatc gcgcttcgcg aacgcctctt ggatcgcggt
     3421 cgcctcggcg ccgaagccgc agtgctcgaa cacggtcgcg tacgacttga ccgtgccgta
     3481 gaaggcgatc atcccggccg cctcgcggcg ggccagctcg gcgtcggggg cggcgcaggc
     3541 gagcgcgtag gtggcgatcg ccacctcgcc agcgtcgcgg ccggtcttcg cggcgccgcg
     3601 ctcgaccgcg gggcgggcga cgtcctccag gtagcgcggc gcgcacatcg tgtgcgcgag
     3661 caggccgtcg ccgacccggc cggcgctctc gatcatccgc ggcccaaccc cggcggtgta
     3721 gatcgggatc tgccgctcgg gcacgcccgc cgactcgtcg agcgcgcgca gcttcagcct
     3781 gtagaagcgg ccttcgtggt cgatcggggc ctcggccagg ttccatacct ggcggaccag
     3841 cggcaccagc tcctccatcc gcaccgccgg cgcgtcgccg tcgaggccgt gccagtcgga
     3901 gatcatccgc cgggtgccgt tgccgatccc gaggatgaag cggccgccgg agagctcgtc
     3961 gaggtcgcgc gcctcggcgg ccagcatcag cgggctgcgt ccgaccccgt agatgatccc
     4021 cgagccgatc gcggcctgtt tggtgcgcgg ggtgatcgcg gcgagggtga cggtggccga
     4081 gcggttgtag agctcggagg tccagaccgc gtcgaagccg gccttgtcga ccgcctcgcc
     4141 ggcggcgctc atcgcctcca gcgtggtccc gaagaccgcg accccgcggc gcgcggcgct
     4201 catcgcggcg cccccgcgct gttgcggcgg cggtcgaagg gcggctcctc ataagggggg
     4261 accgcggcgg gcaggtcggc gctgacggtc agcgggaagc tcggcgggcg cttctccatg
     4321 aacgagtcgg cgccctcctt ggcgtcggcg cccatcgccc gctcggcgat gtagatcgac
     4381 tcctggcggt gcgcgtccat cgggtgggcg gcgccggcca tctgccagag cagctggcgg
     4441 ttgagcgcga ccgagaccgg ggcgctcgcc tcgatcatcc cgcgcgccac ctcgtaggcg
     4501 gcctcgagca gctcctcggg cgggtgcagg gagcggacca ggccgccctc cagcgcctcc
     4561 tcggcgtcga agatgcggcc gctgatcgac cactcgagcg cccggctgag cccgaccgcg
     4621 cgcggcagga accagctcga ggcggcgtcc atgcacatcc cgcgccggct gaagacgaag
     4681 ccgaagcggg ccttctcgga ggcgaggcgg acgtccatcg gcagcgggaa ggtggcgccg
     4741 aagccgaccc cggcgccgtt gatcgccgcc accaccggct tcaggcagcg gaagatccgc
     4801 agcgcgatca ggccgccgga gtcgcgtggg atgtcgcccg gccccagctc ctcggtgccg
     4861 cgcagttcgg ccgcgcggta gacgtcaaag ccggcctcgg tgaggtcgaa gccggcgcag
     4921 aaggcccggc cccggccggt gacgacgacg gcgcgcacgt cgtcgtcctc atcgaccagg
     4981 tcccagacct ggagcagctc gcgctccatc gtcggatcga gcgcgttgag ctgctccggc
     5041 cggttcagcg tgatcgtcgc aatcgcgtct gagacctcga actcgatggt cgtgaacgat
     5101 cggctcttcg gcgagtcgga caacccgcaa ccgagtcttg cggccggcgg cggccaccgg
     5161 aactgacggt tgcgccagtt gaccgtcccg gcacccgcgg ggttgagtta acaggccggt
     5221 accccgactg aggaggagtc cccatgccca ccgatgccct gctcgacgac gcgggcgccc
     5281 ttgcggcggc cgtcgccgga ccgccgatcg gcgactcgat cgccctgcgc cgcgatgagg
     5341 acgggaccgt ggtcacgagc tgcggcgagt gcggttacgc cttcggcccg gccgatcgcg
     5401 acccgaagct cggcgcggtg atgcgcgagc gctcgatcgc cgacctcagc ccgctgaacg
     5461 cggaggggat gaccgagcgc ctcgtcgccc gtcacttcca ctgcccgcag tgcgcgctgc
     5521 tgctcgcggt caacgtccag caggtgggcg acccgatcat gctcgagtgg cgactcgacg
     5581 tcgacgggaa gggggcgtag ccgatgtacg acatcgccgt agacatcgga gggacgttca
     5641 ccgactgcgt gatccacgac gccgagcgcg agctggtgat ggcggccaag gcgccctcgc
     5701 ggcgcgacaa cctcggcggc ggtgtgctcg actcggtcga ggccgcggcc gagcggctcg
     5761 gggtgccgct cgagcaggtg ctcgcgaaca cgcggcgctt catccacggc tcgaccgttg
     5821 ccaccaacgc gatgatcgag cgcgacggcg ctcgcaccgc ctacgtgacc accgccggcc
     5881 acgaggacac gctggcgatc gggaaaatct tccagaagcg cgccgggctc tccgagcgcg
     5941 agatctccca cctcaaccgg ctgcagatgg ccgacccgcc gctggtcccg cgcgagctgg
     6001 tcttcggggt ccaggagcgc atggactacc gcggtcgcgt ggtcgtggcg ctgagcgacg
     6061 cggagatcgc ggcgacgatc gagcgagtgc gcgagtccgg ggtcgagtcg gtcgccgtct
     6121 gcctgctctg gagcttcatc gagcccgagc acgagcggcg cctgaccgag gcgatgcggg
     6181 cggccctgcc cgacgtctac gtctcggcct cggtcgacgt ctcgccggtg ctgggcgagt
     6241 acgagcgcgg cgtgaccacg gcgctgaacg cctacctcgg cccgccgatc tcccgctacc
     6301 tcggcggcct gcaggagctg ctcagcgaga acgggctgag cgcccaggtc ctgctgatga
     6361 ccgccggcgg cggcgtcgtc tcgatcgaca gcgcccgcga caaaccggtg accctgctcg
     6421 actccggccc ggtcggcggc accctcggtt cgcgcttcgt cggctccggc tatgacgagg
     6481 agaacatcat ctgcaccgac gtcggtggca cctcgttcga cgtctcgctg gtgatcggcg
     6541 aggagttcca gctcgagcac gagccggtga tcgcccagta cgtgttcaag atcccgaagg
     6601 tcgcggtcaa gtcgatcggc gccggcggcg gctcgatcgc ctgggtcgac gggctcggcg
     6661 tgctcaaggt cggcccgcag tcggctggtg cccgtcccgg accggcctgc tacggccgcg
     6721 gcggcgagct gccgacggtc accgacgcca acctgatcct cggctatctc aacccggaca
     6781 acttcctcgg cggcgcgatg acgctcgacc gcgaggcggc ggtgcgggct ttcgagccgc
     6841 tggccgagca gctcgggctg agcgtcgagg aggcggccgc gggcgtgcac aagatcatga
     6901 actcgcacat ggccgacctg ctgcgcggct cgacgatcga gcgcggccac gacccgcgcg
     6961 acttcgagct cttcgcctac ggcggcgccg cggcgacgca cgcggtcggc tactcggccg
     7021 aggccggggt gcgctcgatc cggatccccg ccgatgcgac cgcgttcagc gccctcggga
     7081 tgctgaccgc ggacctgacc cactccttcg agcgcggccg cccggtctcg accccgttct
     7141 ccgacgagga cttcgagacg atggccagcg tgttcgccga gctcggcgag caggcgaccg
     7201 agcagctgcg gctcgacggc gaggacccgg aggaggcgac gatgcggcgc agcctgctga
     7261 tgcgcttccg cgcccaggtg cacgagatcg agatcgacgt gccggcggag atctcgctcg
     7321 acgcggccgg gatcgacgcc ctgatcgagc gcttcaccga gcgctacgag cagacctacg
     7381 gggagggctc cgcctacccg gacgccggcg tcgagatcac caccttccgg ctgaccgcga
     7441 cgattccgac ctcgctggat gcaataccag ggttgggtga gcgttccggt agtgccagcg
     7501 aggcgaagac cgggacccgc gacgcctact tcgagggggc ggggttcggc ccggtcgacg
     7561 tctacgacgg cgaacggctg gcgcccggaa accgggtcga gggaccggcg gtgatcgagc
     7621 gctacggcga caccgtcctg atcccgcccg gcctggccgg cgagatggac gggcgcggct
     7681 cgatcacggt cgagatcggc gaggctgcgg tcgcgggcag tgaccgcgac cgggtcagcg
     7741 gcggccgcct cgacccgatc acctacgagg tgatacgcaa ccggctctgg gcgatcaacg
     7801 acgagcaggc ccagacggcg gcccgcaagt ccggctccca gttcatctac gaggcgttcg
     7861 acttcaacgc cggcttgctc gacggcgacg gcaacggtct tttcgccggg gtctacgtcc
     7921 tcttccacac gaccgggctg gacatgaccg tcaaggagat catgcggcgg ttccccgaca
     7981 tctccgacgg cgacgtgttc atcaccaacg acccctgggt cggggcgatc cacttcaacg
     8041 acttcgtcac ggtgacgccg atcttcgccg acggcgagat cgtctcctgg gcggcgctgg
     8101 tgatgcacta ccaggacgtc ggcgggccgg tgccgggcag cttcgtggtc ggctcccaca
     8161 acgtcttcga ggagtgccca atcatcaccc cgctgaagct gatggacaag gggcgctact
     8221 gccaggaggt cgaggatctg ctgctgcgca acacgcgcac gccgatgatg aacgggctca
     8281 acctgcgggc gaccgtggcc tcgcagatca ccaccaagcg acgggtgctg gagatcgtcg
     8341 agcgctacgg ggtcgacgcg ctgaaggcgg cgaccgcgca ggtccaggag gaggtcgagg
     8401 agacggtgcg cggccggctc gccgagatcc ccgacgggga atggtacgac cacgtctacc
     8461 tcgatcacga cggccactcc tacgagctct acgaggtgaa gctgcggctg atcaagcgcg
     8521 acggcaagct gatcttcgac ttcaccggga cctccgagca ggcgccgggg atgatcaacg
     8581 ggacgatctc cgggctgcgc ggcggggtga tgaccgcggt cctggtgatg ctcggctacg
     8641 agatgccgtg gtcgaccggc ggcctgcgca acatcatcga gatcgtctcc gagccgggca
     8701 cgatcaacaa ctgcgagtac ccggccccgt gcagcggcgg ttcggtctgc gccgaggagg
     8761 cgaccgagaa cgtcgccggc accgtgatcg gcaagatgct cggggccagc gccgccctgc
     8821 gcgaggagtc gatgaccgtc tggtacccgt acttcaacat cgtgatcctg gccgggatcg
     8881 accagtacgg ggaaccgctg gcaagcctga tgttcgacaa ctcggccggc ggcggtggtg
     8941 cccgcctctg gggcgacggg gtcgactgcg gcggctactt cgagtcggtc tcctgcgtct
     9001 gcccgaacgt cgagtcgaac gagaagatct tcccggtgct ggagctctac cgccgccggg
     9061 cggcgggcag ctacggccac ggccagcgcc gcggcggcac cgggatcgag ttcgcgacga
     9121 tcagctacga cgtcgacgac gacctcgaag tgatcgtcac caaccacggc gccgcccagc
     9181 cgggggcgcc gggcctctac ggcggcttcc cctgcgcgct gaacacgaac gtgatcctgc
     9241 gcgacgccga cacccgcgag cggctcggcg gcgcgggggc gatctccggt gaggccgagg
     9301 tcagcgccag gggccgcgag gtgctggagg cgaaggcgcg gacgacgctc tcgaccgccg
     9361 acgtgctgat cacccgcaac gagggcgggc ccggctacgg cgacccgctg cgccgcgacc
     9421 cggccgcggt cgccgccgac gttcgcaccg cgctctgcac ctgcgaggac gcggaggaga
     9481 tctacggggt cgcgctcgac ggcgacggct tcgacgccga cgcgaccgcg gcgcggcgcg
     9541 agcagctcaa ggccgagcgg ctcgccgact cggtcccggt cggcgaggcg gtcgcccgct
     9601 acggggtgga gggcgtcggc gccgccaggt gagtctcgag gaaggcgctg aggcgggcga
     9661 ttccctcgct cgcctccggc gtctccgggg cgaacatgtg gtacccgtgg atcgtgccct
     9721 cggcgtcgtt gaggctgact tcgaccccgg cctcgcgcag cgcctcgccg agccggagcg
     9781 cgtcgctgcg gatgccgtcg cggccggaga cctcgatctg gaccggcgag aggccggcga
     9841 ggtcggcgaa gaggggggag gcgtgcgggt gctcggggtc ctggccctgc aggtagttct
     9901 cggccatcgc ctcggccagc gggcgcagtt ccgggttcgg gtcttcgacg ctcgggtcgt
     9961 cgaggctctc ggcgccgttg gtgaggtcgg cccaggggca gagcgagagg tggcaggcgg
    10021 gcaacgggtc gccggcgtcg cgcagcgcga ccatcagcgt gatgcccagc gcgcctccag
    10081 cggagtcccc gccgagcgcg atccgctcgg ggtcccagcc ttgggagagc agccagcggt
    10141 aggcggcgat ggcatcttcg accgcggccg ggaacgggtg ctcgggagcc agtcggtagt
    10201 cgaagttgag caggcgcacg ttcgcttcgc gcgagagccg gccgccgaac tggcgccagc
    10261 tcggggggct gccgatcacg tagccgccgc cgtgcatgta gaggacgacg cgctcctcgc
    10321 cgacgccgtc ccaccactgc cactcgctgt gcggtccccc gatgccaccg acctccgtcg
    10381 cctcccaacg cgtctcggga tcaggcggga gaacgctccc gagagcgtcg taagcggtcc
    10441 gtcccccggc tgggtcgagc gggaacggtc cggcgaattg gcgggcggtg gcttccatct
    10501 cagggcttgg catgggccga ttctctgacg ccacttcgcc ccaggtagct ggcggaatcg
    10561 ccagtagtga gcggccacgg cgatccccta ggctgcgccg atgagctcac gcgcgtttgt
    10621 catcggtgtc ggaatgacca agttcgagaa gcccggccgg cgggagtggg actaccccga
    10681 catggtcgag gaggcgggca cgaaagcgct cgccgacgcg ggcgtcgcct acgacctgat
    10741 cgaccaggtc tacgccggct actgctacgg cgactcttcc tacggccagc gcggcgtcta
    10801 caagctgggg ctgaccgggg tgccgctggt caacgtcaac aacaactgct cgaccgggtc
    10861 cagcgccctc ttcctcgccc gccaggcggt ctccggcggc cacgccgagt gcgcgctcgc
    10921 gatcggcttc gagaagatgg agcgcggctc gctggggatg aagtacgacg accgcgccgg
    10981 cgccctcgac gtccacatcg acgcgatgaa agacgtgcgc gagtgggagg ggtcgccgtt
    11041 cgcgccgcag atgttcggca acgccggccg cgaacacatg gacctctacg gctcgaccgc
    11101 cgatgactac gcctggatcg ggtggaagaa ccacaagcac tcggtcaaca acccctacgc
    11161 gcagttccag gacgagtact cgctggagga cgtgaaggcg gcgaagatga tccacgagcc
    11221 gctgaccaag ctgcagtgct cgccgacctc ggacggctcc gccgccgcgc tggtcgccag
    11281 cgagcgcttc gtcgaggagc acgggctctg ggaccaggcg atcgagatcg ccggccaggc
    11341 gatggtcacc gacctcagct cgaccttcga caccggctcc tgcatcaaga tcgtcggcgg
    11401 cgacatgtcg aaggaggcgg cccggatcgc ctacgagcag gccggggtcg gccccgagga
    11461 cgtcgacgtc tgcgagctgc acgactgctt ctccaccaac gagctgatca cctacgaggc
    11521 gctcggcttc gccgccgagg gtgagggcca gaacctggtc cgcgccgagg cgaccacct
//
LOCUS       DASRIG010000090         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3698913, whole genome shotgun sequence.
ACCESSION   DASRIG010000090 DASRIG010000000
VERSION     DASRIG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698913"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1305
                     /locus_tag="VFB52_02445"
     CDS             61..1305
                     /locus_tag="VFB52_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZO05217.1"
                     /translation="MRGSKGHPGRSRLWFAALALAATALIAAGCGGDDDDSGDSGDPM
                     TVGLSLPLTGEFSQPGKAAKQGYEVWAEKVNEEGGLLDRQVELEIKDDGSDQNTVVAD
                     YNALIQKDDVDLLLGTFSSLLNLPASAAAERNRMLYVEPAGGAPEIFERDFQYGPFFT
                     QQATADHQGDLFAEWVLALPANERPKTVAYAVLDDPFTVPVVDALRATFDDAGIENVY
                     DETYPVSTSNFDSIANSIKSENPDMVVNGSQFEDGIGFRRSLTKVGFEPKILFQTNAP
                     SFGEQYSDGVGVENTEGTFFAISHTPDADTPGNAEFVDAFEQKFGEPPAEDAADAYAA
                     GQVLQAAVEGVGSIEDQLALADWLRENSVETILGPLEWEDTGAPIGEFLIGQWQNGTA
                     EIVLPEDAATSDKIIAVRPQAE"
     gene            1386..2267
                     /locus_tag="VFB52_02450"
     CDS             1386..2267
                     /locus_tag="VFB52_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZO05218.1"
                     /translation="MTEFIQATAFGLLIGGVYALFASGLTLIFGVMKVINIAHGALLI
                     LGAFLAFTLWEQFGLDPLASILITAPAMFVLGLALYVAMIGPLRSAPLSSTVLLTFGL
                     ALVIEALMGLVWGNNSRSARPSYFDQSFDLSGIILPKAQVYASIVAILVLALLWLFLN
                     RTWTGRAIRAASANVQGAALVGVNIARVSALTFAVGLATVGAAGSMVSVLYPFLPGSH
                     YIWIARVLSIIVLGGLGSLQGAVLGAMLLGLAEVYTQTYISTAWATAVPYVLIFVVLF
                     FRPQGLMGTRLREDVAV"
     gene            2264..4090
                     /locus_tag="VFB52_02455"
     CDS             2264..4090
                     /locus_tag="VFB52_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZO05219.1"
                     /translation="MSTVDTVERIGLDRRLWRGVIAVAAVLLIAYPLLSDDLFYQNMI
                     ILSLVLAVGAVGLNIIMGYAGYISLGQSAFIGLGAYTVGIATTRIGGDPFVWVPLAGL
                     VAAAFAALLGVIAMRTRGFAFVIITIAALFILQIVAINWDSLTQGTSGITLPLPRWDI
                     SIQNWPFYYTLIALLGASLLLSWWIRRTKIGTGLLAIREDEDKAATIGIDTPVYKIIG
                     FMASAVFLGMAGGVYGYYIAFIDPTAMFDIVISVQIILAVILGGRGTLWGPVLGAATI
                     EPLNELANQQFGGGNSRLLIFGGLLTLTILLLPKGIIPSVRDLLERRTKRGRAALVGG
                     RLGERRALNGRPATATGGTGRNLLEVKGLHKHFEGVRALDGCSFAVEEGTITGLIGPN
                     GSGKTTAFNLIGGTMRADAGEVWFDGRRIDRLSPWRRAYAGLGRTFQITRVFTDMTVL
                     ENVVAPLRSFSWRQLRAGAVSGEEAARAEELLAFAGMAEYRDERAEALSFGQRKLVEL
                     AQVLMLDPKLILLDEPAGGINPTLIARIAEMIRELNAQGKTFLIVEHNMPLVLGLCEP
                     VLVLASGECISTGTPDEIQRDQQVLDAYLGADFQLEEPVAAP"
     gene            4087..4842
                     /locus_tag="VFB52_02460"
     CDS             4087..4842
                     /locus_tag="VFB52_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05220.1"
                     /translation="MSTMLRLDGVVAGYGAGDVLQGVDLTVDGGSVTCIVGPNGAGKS
                     TVLRAISGLLRPSNGAIVYQEQEIHRRAPDEILELGVTQVPQSDALFANLSVRENVLM
                     GAYIARRDRKLVERRYAEVSELFPLVGERSDERAGNLSGGQRRSVEFARSLMLDPSLV
                     MLDEPSVGLDPKALKVVAAAMKRMVEAGKTLLLVEQNVRFGLRMSTHGVVMESGRVLM
                     HDRADAVLNNPEMTDLFFGGTPTKAPPPTAARA"
     gene            4839..5711
                     /locus_tag="VFB52_02465"
     CDS             4839..5711
                     /locus_tag="VFB52_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO05221.1"
                     /translation="MSGAGWAALSGAGFGLFQAINARAVRDLDSIYFSTFLQLVLATV
                     VLAIASVATEDLSELADATAWGIIAFALAGLVHFFIGWTTLNVSQARIGAARTSPLLS
                     TSPVFGVVFAAVFASQLPDALAAFGIALTIAGAYLVADPGAGERAPLSDSIFALATAC
                     AWALSAVLTVEGLEGFSSPLLGVTVGMLASTVAYGVLLPFSSEPIRLGDRGALELKLL
                     AGLIVGIATWGRWVALDDASVGAVLALQLLAVPVVLVAAPLISGRHVEIVTTKIWAGA
                     ALVIVGSLLLILAE"
     gene            5719..>6288
                     /locus_tag="VFB52_02470"
     CDS             5719..>6288
                     /locus_tag="VFB52_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HZO05222.1"
                     /translation="MVATPNGPMQAAVVERVGEPPEVGRIEPPHESANGTLVAPRYGT
                     LNPIEVRIAAGREGAAPEVPYVPGMEGVGRVVSGDGLDAGSAVRFECRLPGRGSDGAL
                     AELAVAEPGSLVALPDGIDEGLAAAVGVIGVTADLALDAAAPIAGARVVVLGASGSVG
                     RIAVQLAKQRGAARVVAAGRDRAALRRAVE"
BASE COUNT          892 a         2168 c         2226 g         1002 t
ORIGIN      
        1 gagaaggtgg atgcgagccc cggtggccgg tgagcgccgc gacgtgctgg gaggtacgtg
       61 atgagaggat cgaaggggca cccggggcgg tcgcggcttt ggttcgcggc actggccctg
      121 gcggccacgg cgctgatcgc cgctggctgc ggcggagacg acgacgacag cggcgacagc
      181 ggcgacccga tgacggtcgg gctctccctg ccgctcaccg gcgagttctc gcagccgggc
      241 aaggccgcga agcagggtta tgaggtctgg gccgagaagg tgaacgagga gggtggactc
      301 ctcgaccgcc aggtcgagct ggagatcaag gacgacggca gcgatcagaa caccgtcgtc
      361 gccgactaca acgccctgat ccagaaggac gacgtcgacc tgctcctcgg gacgttctcg
      421 tcgctgctca acctcccggc gtcggccgcc gcggagcgaa accggatgct ctacgtcgag
      481 ccggccggcg gcgcgccgga gatcttcgag cgcgatttcc agtacgggcc gttcttcacc
      541 cagcaggcca ccgcggatca ccagggcgac ctgttcgcgg agtgggtcct cgccctgccc
      601 gcgaacgagc ggccgaagac ggtggcctac gcggtgctcg acgatccgtt cacggtgccc
      661 gtcgtcgacg cgctccgggc cacattcgac gacgccggga tcgagaacgt ctacgacgag
      721 acctacccgg tcagcacgtc gaacttcgac agcatcgcca actcgatcaa gtcggagaac
      781 ccggacatgg tcgtcaacgg gtcccagttc gaggacggga tcggcttccg ccggtcgctc
      841 accaaggtcg gcttcgagcc caagatcctg ttccagacca acgcgccgtc gttcggcgag
      901 cagtactcgg acggcgtcgg ggtcgagaac accgagggca cgttctttgc gatcagccac
      961 acgcccgacg cggacacgcc gggcaatgcc gagttcgtcg acgcgttcga gcagaagttc
     1021 ggcgagccgc cggccgagga cgccgccgac gcctacgcgg ccggacaggt gctgcaggcc
     1081 gcggtcgagg gcgtcggcag catcgaggat caactcgcgc tcgccgactg gctgcgcgag
     1141 aacagcgtcg agacgatcct cgggccgctc gagtgggagg acacgggggc cccgatcggt
     1201 gagttcctga tcggccagtg gcagaacggc actgccgaga tcgtcctgcc cgaggacgcg
     1261 gccaccagtg acaagatcat cgccgtgcgg ccgcaggcgg agtaagggag gaacgcatgg
     1321 tcgcggcgac cgcggggtat tcgcaccccc gcggtcgccg ctgagccgca gggacggccg
     1381 ccaagatgac cgagttcatc caagcgacgg cgttcggcct cctcatcggg ggcgtgtacg
     1441 ccctgttcgc ctccgggctg acgctgatct tcggcgtgat gaaggtgatc aacatcgcgc
     1501 acggggcgct gctgatcctc ggcgccttcc tggcgttcac cctctgggag cagttcgggc
     1561 tcgatccgct ggcctcgatc ctgatcaccg cgccggcaat gttcgtactc ggcctggccc
     1621 tctacgtggc catgatcggc ccgctgcgct cggcgccgtt gtcatcgacg gtgctgctga
     1681 ccttcgggct cgcgctcgtc atcgaggcgt tgatggggct ggtctggggc aacaattcgc
     1741 gctcggcacg gccctcgtac ttcgaccagt ccttcgacct ctcggggatc atcctgccga
     1801 aggcgcaggt gtacgccagc atcgtcgcga tcctcgtcct ggccctgctc tggctgttcc
     1861 tcaaccggac gtggaccggc cgtgcgatcc gggccgcgtc cgcgaacgtg cagggggcgg
     1921 cgctggtcgg ggtcaacatc gcgcgggtca gcgcgctcac cttcgccgtc gggctcgcca
     1981 cagtcggcgc cgccgggtcg atggtctcgg tgctctaccc gttcctgccg ggctcccact
     2041 acatctggat cgcccgcgtg ctcagcatca tcgtcctggg cgggctgggc agcctccagg
     2101 gggccgtgct cggcgcgatg ctgctgggcc tcgccgaggt ctacacgcag acctacatat
     2161 caaccgcctg ggcgacggcg gtgccgtacg tgctgatctt cgtcgtgctc ttcttccgtc
     2221 cccagggact gatggggacc cgcctgcgcg aggacgtggc ggtatgagca ctgtcgacac
     2281 cgtcgagcgc atcggcctcg accgcaggct ctggcgcggg gtgatcgccg tcgcggcggt
     2341 gctgctgatc gcctatccgc tgctcagcga cgacctcttc taccagaaca tgatcatcct
     2401 ctcgctcgtc ctggcggtcg gtgcggtggg gctgaacatc atcatgggct acgcgggcta
     2461 catctcgctg ggccagagcg cgttcatcgg cctcggcgcc tacacggtcg ggatcgcgac
     2521 gacgcggatc ggcggcgacc ccttcgtctg ggtccccctc gcggggctgg tcgccgccgc
     2581 tttcgcagcg ctgctcggag tgatcgcgat gcgaacgcgc gggttcgcct tcgtgatcat
     2641 cacgatcgcc gccctgttca tcctccagat cgtcgcgatc aactgggact cgctgacgca
     2701 gggaaccagc ggcatcaccc tgcccctgcc gcggtgggac atctcgatcc agaactggcc
     2761 cttctactac acgctgatcg ccctcctcgg cgcctcgctg ctgctctcgt ggtggatccg
     2821 gaggacgaag atcggcaccg gcctgctggc gatccgcgag gacgaggaca aggcggcgac
     2881 gatcgggatc gacaccccgg tctacaagat catcggcttc atggccagcg ccgtgttcct
     2941 cggcatggcg ggcggcgtct acggctacta catcgccttc atcgacccga ccgcgatgtt
     3001 cgacatcgtg atcagcgtcc agatcatcct cgcggtgatc ctcggcgggc gcgggacgct
     3061 ctgggggccg gtgctcggcg cggcgacgat cgagccgctg aacgagctcg cgaaccagca
     3121 gttcggcggc ggcaactccc gactgttgat cttcggcggg ctgctgacgc tgacgatcct
     3181 gctcctgccg aagggaatca tcccctcggt ccgcgatctc ctggagcggc gtacgaagcg
     3241 gggccgggcg gcgctcgtcg gcggccgcct gggcgagcgg cgggcgctca atggaagacc
     3301 ggcgacggcg accggaggga ccggtcgcaa cctgctcgag gtcaagggcc tgcacaagca
     3361 cttcgagggc gtccgggcgc tcgacggctg ctcgttcgct gtcgaggagg ggacgatcac
     3421 cggcctgatc ggacccaacg ggtccggcaa gaccacggcc ttcaacctga tcggcggcac
     3481 gatgcgggcc gacgccggcg aggtctggtt cgacggccgc cgaatcgatc gcctttcacc
     3541 ctggcggcgt gcctacgcgg ggctggggcg gacgttccag atcacgcgcg tgttcacgga
     3601 catgaccgtg ctcgagaacg tcgtcgctcc gctgcgcagc ttctcctggc gccagctacg
     3661 cgccggcgcg gtcagcggcg aggaggccgc ccgggccgag gaactgctgg ccttcgcggg
     3721 catggccgag taccgcgacg agcgcgccga ggcgctctcg ttcgggcagc gcaagctggt
     3781 cgagctggcc caggtgctga tgctcgatcc gaaactgatc ctcctcgacg agcccgccgg
     3841 gggcatcaac ccgacgctca tcgcgcggat cgcggagatg attcgcgagc tcaacgccca
     3901 gggcaagacc ttcctcatcg tcgagcacaa catgccgctc gtgctcggcc tctgcgagcc
     3961 cgtcctcgtg ctcgcgtccg gcgagtgcat ctccaccggc acgccggacg agatccagcg
     4021 ggaccaacag gtcctcgacg cctacctcgg cgccgacttc cagctcgagg agccggtggc
     4081 agcgccatga gcacaatgct gcgcctcgac ggagtggtgg ccggctacgg cgccggcgac
     4141 gtgctgcagg gcgtggatct gaccgtcgac ggtggctcgg tgacctgcat cgtcgggcca
     4201 aacggcgccg ggaagtcgac cgttctgcgg gcgatcagcg gactgcttcg cccgagcaac
     4261 ggcgcgatcg tctaccagga gcaggagatc caccgccgag cgccggacga gatcctcgag
     4321 ctcggcgtca cccaggtccc ccagagcgac gccctgttcg cgaatctctc ggtacgcgag
     4381 aacgtgctca tgggcgccta catcgcgcgc cgcgatcgca agctggtgga gcgtcgttac
     4441 gccgaggtgt ccgagctctt cccgctggtc ggtgagcgct ccgacgagcg cgccggcaac
     4501 ctgtcgggtg gccagcggcg ctcggtcgag ttcgcgcgct cgctgatgct cgatccgtcg
     4561 ctggtgatgc tcgacgagcc ctcggtcggc ctcgacccca aggcgctcaa ggtggtcgcc
     4621 gccgcgatga agcggatggt cgaggcaggc aagacgctcc tcctcgtcga gcagaacgtc
     4681 cggttcggcc tgcggatgtc gacccacggc gtggtcatgg agagcggccg cgtactcatg
     4741 cacgatcgag cggacgcggt cctcaacaac ccggagatga ccgacctgtt cttcggggga
     4801 acgccgacca aggcgccgcc gccgacggcg gcgagggcgt gagcggcgcc ggatgggcgg
     4861 cgctctcggg agcgggattc ggcctctttc aggcgatcaa cgcccgtgcc gtccgcgacc
     4921 tcgacagcat ctacttctcg accttcctcc agctggtcct ggcaaccgtc gtcctcgcga
     4981 tcgcgtcggt ggcgaccgag gacctctccg agctcgccga cgcgaccgcg tgggggatca
     5041 tcgcgtttgc gctcgccggc ctggtgcact tcttcatcgg ctggacgacg ctgaacgtca
     5101 gccaggcgcg gatcggggcg gcgcgcacct ccccgctcct ctcgacgagc ccggtgttcg
     5161 gcgtcgtctt cgccgcggtc ttcgccagcc agctgccgga cgccctggcg gcgttcggaa
     5221 tcgcactgac gatcgcaggc gcctacctgg tcgccgaccc cggcgccgga gagcgtgcgc
     5281 cgctgtcgga ctcgatcttc gcgctggcaa cggcctgcgc ctgggccctg agcgcggtgc
     5341 tcaccgtcga gggcctggag ggtttcagct cgccgctgct cggcgtcacc gtcggcatgc
     5401 tcgcgtcgac ggtcgcctac ggggtgctgc tcccgttcag ctcggagccg atccggctcg
     5461 gcgaccgcgg ggcgctcgag ctcaagctgc tggccggcct gatcgtcggg atcgccacct
     5521 ggggccgctg ggtggcgctc gacgacgcct ctgtcggcgc ggtgctggcg ctgcagctgc
     5581 tggccgtgcc cgtggtgttg gtcgcggccc cgctgatctc cggccggcac gtcgagatcg
     5641 tgacgaccaa gatctgggcc ggcgccgcgc tggtcatcgt cggctcgcta ctgctgatcc
     5701 tcgcggagtg aggccgcggt ggtagcgacc ccgaacgggc cgatgcaggc ggccgtcgtc
     5761 gagcgggtcg gagagccgcc ggaagtcggc cggatcgagc cgccacacga gagcgcgaac
     5821 gggaccctgg tcgcgccccg ctacggaacg ctgaacccga tcgaggtccg gattgccgcg
     5881 gggcgcgagg gcgcggcgcc ggaggtcccc tacgtcccgg gcatggaggg cgtcgggcgg
     5941 gtggtctcgg gcgatgggct cgatgccggg tcggctgtgc ggttcgagtg ccgacttccg
     6001 gggcgcggct cggacggcgc cctcgccgag ctcgcggtcg ccgagccggg ctccctcgtc
     6061 gcgctgccgg atggcatcga cgagggactc gcggccgcgg tcggggtgat cggcgtcacc
     6121 gccgacctcg cgctcgacgc cgccgcgccg atcgcgggtg cgagggtcgt cgtgctcggc
     6181 gccagcggca gcgtgggcag gatcgcggtc cagctcgcca agcagcgcgg cgccgcgcgg
     6241 gtggtcgcgg ccggacgaga tcgcgccgcc ctgcggcgcg cggtcgag
//
LOCUS       DASRIG010000091         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_567311, whole genome shotgun sequence.
ACCESSION   DASRIG010000091 DASRIG010000000
VERSION     DASRIG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567311"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFB52_02475"
     CDS             <1..415
                     /locus_tag="VFB52_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05223.1"
                     /translation="FAEAWPWNERIDTADDIFMGALWRSHGVRLLFHGDARALHPELP
                     ANSDPERIGATVRHQRCHIYALLFDALIANPSLRHALSYELLGFAASAKLYFRRLSWT
                     VDFLRAWAAGHAGLVSDYSYLRRLVKTEAAPAPGE"
     gene            423..1172
                     /locus_tag="VFB52_02480"
     CDS             423..1172
                     /locus_tag="VFB52_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZO05224.1"
                     /translation="MSGLLILNADDWGYDDPTTAAIVDCYERGGLTSTTQMAFMRGTD
                     AAARVAERVPGLGIGLHLNLVEAYTDPNTPADVRERQRSLIEHARLLRLRRWVYDPFV
                     KDTINQVIADQFRQFIALYGRMPTHVDGHHHCHLAANVLLSPAMPKGTKIRNALSDAH
                     FSNPLTNGLRLAREKLIERRFRTTDYFVSIETTWPGLAGPVPPDKLELARTQSIEVML
                     HPIFDRELEPLASPEWLDAVAAMPTGTYGDL"
     gene            1177..2148
                     /locus_tag="VFB52_02485"
     CDS             1177..2148
                     /locus_tag="VFB52_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO05225.1"
                     /translation="MDGSPARSISVVVPTHNRSERLPALLDALAPQESEVVVVANACT
                     DDTVAALERRAAADPRLVVLEREQAGQLGALQAGVEQAGGDVVLLLDDDVIAAPGLLA
                     GHLAHHAARPGLVVLGYMPIALPPRRRAGEFPLDLYAREYEKACDRYESDSATVLAGL
                     WGGNVSLRREDALRVGLVPTEALLEGYGYHEDQDFGLRCAAAGLAGVFDRALLAEHRY
                     ERSPDSFLAAARNSGATRFALYQRYGDEQPLTPDHYEQTAPFPGKLLVRWARRRGARS
                     LIQGFLKGVIAVAGALHLFRLESHAGFLLGTVEQQRGAFDARNQAQR"
     gene            2153..3235
                     /locus_tag="VFB52_02490"
     CDS             2153..3235
                     /locus_tag="VFB52_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZO05226.1"
                     /translation="MEEKRQRRWALIPLVLVGAIVLLLPLSAALDLPLERQVKDALGL
                     DYKEEGCAPIHDRGVWGAWRKEGHTPTLLEEGRAVTVGDSAYLVGGLTTQVVDNFGKS
                     TAAFRRYDFDTGKFESLPDLPERLNHVGMAAQGNDVYVVGGLGDQLEFLSEASPALFR
                     YDIPSQTWVEMPPMRTPRGALGAAFVGDDLFAVGGRNGTDSYATVEMFDTKTDRWSNR
                     APLPGGRDHLGVASLGGFVYAVGGRYDGGPELAEFLRYNPRTNEWKRLQDLPTGTSGV
                     TLERVGDELAITGGEDSEQAWVTGRTFAYSPQTGRWRNLADSPRPKHGYASTGHDGRL
                     YVFGGAICAGSTPTDTIESLRVSKGS"
     gene            3232..3498
                     /locus_tag="VFB52_02495"
     CDS             3232..3498
                     /locus_tag="VFB52_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05227.1"
                     /translation="MTEHRVSARLGLTPAEAELLIGRAVERLGRRGYELRACAFLLAT
                     GGTLRPAIPRRLRLRAIRAFLHCLLGGASENLLYAGAIGVGTGR"
     gene            3495..>4376
                     /locus_tag="VFB52_02500"
     CDS             3495..>4376
                     /locus_tag="VFB52_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZO05228.1"
                     /translation="MSESADVLVVGSGISGLLVARELLAAGREVTIVERGPLRLDADK
                     LPLEEREAALPTTAHNTEAPPGEHIHGWQYGYAYGGSSLLWAGVAPRLLPSDFELHTR
                     HGVGRDWPISYDDLIPFYQEAERALRVCGRPDPAFPGTDAYLEPPRPSDADLLVGPLL
                     EPFGTLPIARELAAPGQYPPPLEGDASAVERSVTILGVAREVADSPGLRVVDRSAAAR
                     LRTAAGQVEAVECVGADGDKFELRANQVVLATHGIENPALLLRSGLDGPAVGRWLGDH
                     THVILDVELAEPHRHWDA"
BASE COUNT          624 a         1704 c         1435 g          613 t
ORIGIN      
        1 gttcgccgag gcctggccct ggaacgagcg gatcgacacc gccgacgaca tcttcatggg
       61 cgcgctctgg cgtagccacg gcgtgcgcct gctctttcac ggcgacgccc gcgccctgca
      121 ccccgagctg ccggccaact ccgaccccga gcggatcggc gccaccgtgc gccaccagcg
      181 ctgccacatc tacgccctgc tgttcgacgc cctgatcgcc aacccgagcc tgcgccacgc
      241 gctctcctac gagctgctcg ggttcgcagc cagcgccaag ctctacttcc gccggctctc
      301 ctggaccgtg gacttcctgc gcgcctgggc cgctgggcac gccgggctgg tctccgacta
      361 ctcctatctg cgccgcctgg tcaagaccga ggccgccccg gcgccgggcg agtgaggcaa
      421 cgatgagcgg cctgctgatc ctcaacgccg acgactgggg ctacgacgac ccgaccaccg
      481 cggcgatcgt cgactgctac gaacgcggcg gcctaaccag caccacccag atggccttca
      541 tgcgcggcac cgacgccgcc gcccgcgtcg ccgagcgggt gccgggcctc ggaatcggcc
      601 tgcacctcaa cctggtcgag gcgtacaccg acccgaacac tcccgccgac gtgcgcgagc
      661 gccagcgcag cctgatcgaa cacgcgcgcc tgctgcgcct gcgccgctgg gtctacgacc
      721 cgttcgtcaa agacacgatc aaccaggtga tcgccgacca gttccgccag ttcatcgcgc
      781 tctacgggcg catgccgacc cacgtcgacg gccaccacca ttgccacctg gccgctaacg
      841 tgctgctttc cccggcgatg ccgaaaggca caaagatccg caacgcactc agcgacgccc
      901 acttctccaa cccgcttacc aacggcctgc gcctggcgcg cgagaagctg atcgagcggc
      961 gcttccggac caccgactac ttcgtctcga tcgagaccac ctggcccggg ctggcggggc
     1021 cggtgccgcc ggacaagctt gagctggcgc gcacccagag catcgaggtg atgctgcacc
     1081 cgatcttcga ccgcgagctg gagccgctcg cctcgcccga gtggctggac gcggtagcgg
     1141 cgatgccgac tggtacctac ggggatcttt gaggcgatgg acggatcgcc cgctcgctcg
     1201 atctcggtcg tcgtccccac ccacaaccgc agcgaacgcc tacccgcgtt gctcgacgcg
     1261 ctcgccccac aggagagcga ggtcgtggtc gtcgccaacg cctgcaccga cgacacggtc
     1321 gcggcgctcg aacgacgcgc cgccgcggac ccgcggctgg tcgtgctgga gcgcgagcag
     1381 gcgggccagc tcggcgccct gcaggcgggg gtcgaacagg ccggcggcga cgtcgtcctg
     1441 ctgctcgatg acgacgtgat cgccgcgccg ggactgctcg ccggccacct cgcccaccac
     1501 gccgcccgcc ccggcctggt cgttctcggc tacatgccga tcgcgctgcc gccccggcgc
     1561 cgcgcgggcg agttcccgct cgacctctac gcgcgcgagt acgaaaaggc ctgcgatcgc
     1621 tacgaaagcg actccgccac cgtgctcgcc gggctctggg gcggcaacgt ctccctgcgc
     1681 cgcgaggacg cgctgcgggt cggcctcgtc cccaccgaag cgctgctcga gggctacggc
     1741 taccacgagg accaggactt cggcctgcgc tgcgcggccg ccggcctcgc gggcgtcttc
     1801 gaccgcgcgc tgctcgccga gcaccgctac gaacgcagcc ccgattcctt cctcgccgcc
     1861 gcccgcaact cgggcgcgac ccgcttcgcc ttgtaccagc gctacggtga cgagcagccg
     1921 ctcactcctg accactacga acaaactgcg ccgtttccag gtaaattgct cgtccgctgg
     1981 gcgcgcaggc gcggcgcccg gtctctcatc cagggcttcc tgaaaggggt catcgcagtg
     2041 gccggagcgc tccacctgtt ccgcctcgag tcccacgccg gtttccttct cggcacggtc
     2101 gaacagcaac gcggtgcctt cgatgcgcgg aaccaggccc agcggtgacc tgatggagga
     2161 gaagcgacag cgccgctggg ccctgatccc gctcgtgctc gtcggcgcga tcgtcctgct
     2221 cctgccgctt tcggcggccc tcgatctgcc gctcgagcgc caggtcaaag acgctctcgg
     2281 cctcgactac aaagaggagg gctgcgcgcc gatccacgac cgcggcgtct ggggcgcctg
     2341 gcgcaaagag ggccatacgc cgacgctgct cgaggagggc agggcggtaa ccgtcggcga
     2401 cagcgcgtac ctggtcggcg gcctgactac ccaggtggtc gacaacttcg gcaaatcgac
     2461 cgccgccttc cgacgctacg acttcgacac cggcaagttc gaaagcctgc cggacctccc
     2521 cgagcgcctg aatcacgtcg gcatggccgc gcagggcaac gacgtctacg tggtcggcgg
     2581 cctcggcgac cagctcgagt ttctctccga agcctcgccg gcactgttcc gctacgacat
     2641 cccgagccag acctgggttg agatgccgcc gatgcggacg ccccgtggcg cgctcggcgc
     2701 ggcctttgtc ggcgacgatc tcttcgcggt cggcgggcgc aacggcaccg actcttacgc
     2761 cacggtcgaa atgttcgaca cgaaaaccga ccgctggtcg aaccgggccc ccctaccggg
     2821 cggccgcgac cacctcggcg tcgcctcgct ggggggcttc gtctacgccg tcggcggtcg
     2881 ctacgacggc ggtcccgagc tcgcggaatt ccttcggtac aacccgcgca cgaacgagtg
     2941 gaaacgcctc caggacctgc cgaccggaac cagcggcgta accctcgagc gggtcggcga
     3001 cgaactcgcg atcaccggcg gcgaggactc cgaacaggcc tgggtgaccg gccgcacctt
     3061 cgcgtactcc ccgcagaccg gtcgctggcg gaacctcgcc gacagccccc ggccgaagca
     3121 cggctatgcc agcaccggcc acgatgggcg cctctacgtg ttcggagggg cgatctgcgc
     3181 cggctcgacc ccgaccgaca cgatcgagtc ccttcgggtc tcgaagggca gttgaccgag
     3241 caccgcgtca gcgcccgtct gggtctgacc ccggccgagg ccgagttgct gatcgggcgc
     3301 gcggtcgagc gcctcgggcg gcgcggctac gagctgcgcg cctgcgcctt cctgctcgcc
     3361 accggcggca ccctgcgtcc ggcgatcccg cggcggctgc gtctgcgggc gatccgcgcc
     3421 ttcctgcact gcctgctcgg cggggccagc gagaacctgc tctacgcggg cgcgatcggg
     3481 gtagggaccg gcaggtgagc gagagcgcgg acgtcctcgt cgtcggctcc gggatcagcg
     3541 gcctgctggt cgcgcgcgag ttgctcgccg ccggccgcga ggtgacgatc gtcgagcgcg
     3601 gcccgctgcg tctcgacgcc gacaagctgc ccctggagga gcgcgaggcg gcgctgccga
     3661 cgaccgcgca caacaccgag gccccgcccg gcgaacacat ccacggctgg cagtacggct
     3721 acgcctacgg cggctccagc ctgctctggg ccggggtcgc gccgcgcctg ctcccctccg
     3781 acttcgagct gcacacccgc cacggggtcg gccgcgactg gccgatctcc tacgacgacc
     3841 tcatcccctt ctaccaggag gccgagcggg cgctgcgcgt ctgcggccgc cccgatcctg
     3901 ccttcccggg gaccgacgcc tacctggagc cgccgcgccc ctccgacgcc gacctcctgg
     3961 tcgggccgct gctggagccg ttcgggaccc tgccgatcgc ccgtgagctg gccgcgccgg
     4021 gccagtaccc gccgccgctc gagggcgacg ccagcgcggt cgagcgctcg gtgacgattc
     4081 tcggcgtcgc ccgcgaagtc gcggactccc ccggcctgcg cgtggtcgac cgcagcgccg
     4141 ccgctcgcct gcgcaccgcc gccgggcagg tcgaggcggt cgagtgcgtc ggcgccgacg
     4201 gcgacaagtt cgagttgcgc gccaaccagg tcgtgctcgc gacccacggg atcgagaacc
     4261 cggcgctgct cctgcgctcc ggcctcgacg gccccgcggt cggccgctgg ctcggcgacc
     4321 acacccacgt gatcctcgac gtcgagctgg ccgagccgca ccgccactgg gacgcc
//
LOCUS       DASRIG010000092         8083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3699235, whole genome shotgun sequence.
ACCESSION   DASRIG010000092 DASRIG010000000
VERSION     DASRIG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699235"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFB52_02505"
     CDS             complement(<1..718)
                     /locus_tag="VFB52_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZO05229.1"
                     /translation="MSARRRRAMRQWVLWALAACALAGALLPTWAVSAAPDEEAKGTL
                     DRAVRFLQEAQNLDGGFGGEVGGASDPGFSSWAAYALAAAGINPQDQSQQGGVDVYTY
                     LTRNTDGLEQTTDFDRVALVAIASDTSPFEFGRVRPIERMLERQLPDGSFTQKPGQKQ
                     GWVNATVWSIFPLSTIDARWAEESVRRAAQWLLDNQRSDGGWGWGAGGDGGLVDTDTT
                     GAVIQALNAAGMHGSEAERKA"
     gene            complement(715..1869)
                     /locus_tag="VFB52_02510"
     CDS             complement(715..1869)
                     /locus_tag="VFB52_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05230.1"
                     /translation="MTVRIEGAEKTLFEGPIATEGHDVQATSDAEPRRCDATNNGAHP
                     DPGPTPTAASVDAMELIGQTFNGEWYPGFDDYFIQRWGPDAEDEEAFAYWGILVNGAF
                     TPVGGCQYLAEEGDEVFWVYDAFMGRQLLWLSAAGDLGFPAAPTASVEAGEPLELVVE
                     KGEQPSPAAAEGITIAPVATAEGTGYQTVETGSSAAVVTDAEGEASITFSTPGWHRIK
                     AQKEAGFLRSNRLDVCVEPVGGGSCGTPPADSLVRTPPRYLEDEEEPQHPGGGGTPPP
                     PVQDPPKPSNLIGLRKLTLNRGKGTATLKVALPGAGQLLASGAKVATQRLSATAAKVV
                     TVLVKPKTKARAELLAKGRLAVSVRLQFTPSGGEPGTKTRALTLKLSGGG"
     regulatory      complement(1980..2187)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2233..3627)
                     /locus_tag="VFB52_02515"
     CDS             complement(2233..3627)
                     /locus_tag="VFB52_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05231.1"
                     /translation="MRHTTWRLALLAAALVALLVPSSALATATAEEITGSIGKGLTYV
                     KGLQNKETGAIPGFGGDWALTAFAATKVAAADVNKGGKATTDARSWYEAEVGPAGWPG
                     EGATATDYERAALLAYAAGIDPARVSKRQNLIAKVASYYQPETPGYYGSTLNATVFGL
                     LTLANVKTTGGVVRVPQAVLDDAVEAVRANQHDDGGWTWSIVAGNPEAEAASSEPDMT
                     GAAIAALCGAGLPKTNATIEAAKGYLVSIFEGSSGAFENEFGNNTDSNAWVVQGLKAC
                     GIDPQGAEFTGPSPNKKTPLNFLISNQLGSGAFRYGTSGSTANLYATQDALRALGSGG
                     FTAAPPVPSGGAAQWKGVTQFATGEAETTSLALIVDDGVSALKVCSVTLAPKATTTNL
                     ATVLNAAVAGSSPAGCLSGFLPASGEGAITQVNGYPAVPAEKWKVSIDGGAAATAKRS
                     TAINVGDTIHLYAE"
     regulatory      complement(3754..3830)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      3882..4088
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4153..5646
                     /locus_tag="VFB52_02520"
     CDS             4153..5646
                     /locus_tag="VFB52_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05232.1"
                     /translation="MIVLPAVAQAASQTEIDAAIDKAVAYAASKQDPATGAIEDFGGD
                     WMVTALTAAGVDAGDFRVEAGDPSLQDSYLGEYTLPTWTEDPPSGTVNEYSRAVLFAY
                     AAGLDPARISADSNQPAQLAGRWNPKTGSFGENSSNNAAFAILAMRTAGLPSWALAPT
                     VDFLRRNQHDDGGWTFTASLDPASQAEPSEADMTGAAVAALCEAGVPTYDPDVAAALT
                     YLQELVISGTGGIEYIWGPPNSDVNAWVISGLHACGLDPQGAEWTVGGKNPVDFLLTL
                     QVPEPAEGAGGFGYMDTSEASLYSTQDALRALAGAAFTADPQSVLAPPSVAAGTPVPH
                     LLAIELAPGNVRLCKVTAPAGAPLTDLLEVAETASRPAGCVHSFAVDGGKVTAIDGVT
                     PDGADEAWLVRLDRGAAAAAGEQPVGFGDLVTLRLGRNPSSEQGPAGPTGPAGASGPV
                     GAKGEPGPRGKRGARGKPGRNATLACKQRRSRAGKPKVRCKVVRAKR"
     gene            complement(5643..6143)
                     /locus_tag="VFB52_02525"
     CDS             complement(5643..6143)
                     /locus_tag="VFB52_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05233.1"
                     /translation="MRARSLTCACLAAIAAALLVAGCGGGREEDTPVACLEGAAAYKQ
                     ALAKAPAAVQLEGTTPIEDCLSENQDGGQLAIVGEAMIEVATELNAEARQDPGNKATL
                     QLGYLIGAAERGAEKTEGIHADLIRRLVVAARFAPGGEPLSPAFLAVYKEGFDAGRQG
                     GWLPDS"
     gene            complement(6208..6888)
                     /locus_tag="VFB52_02530"
     CDS             complement(6208..6888)
                     /locus_tag="VFB52_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05234.1"
                     /translation="MRIFFATAWTALLCLTLGFGVGTAAADAAEAPPSETRLAYGGKI
                     VPIPNSIPHEAGDMVDSRIIPDLKWLAQRYGIYVTDGYSGPAPGGGWAGCHGCHVRGS
                     DHYNGLGVDIVPAEPSSRCDAKWTAISRLARWAEPVQNKPVLPFRWVGYDGDSGHGCG
                     HHLHLSWNHASAKQYEVAQWVEVFSPPGSATAKQPKGKKAKKAPKQPAGPTGGISQYK
                     TGGIGAGD"
     gene            6976..>8083
                     /locus_tag="VFB52_02535"
     CDS             6976..>8083
                     /locus_tag="VFB52_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZO05235.1"
                     /translation="MTTVRDATFDLFRAHGMTTMFGNPGSTELPMLKDYPSDFSYVLG
                     LQEAVVVGMADGFAQASGKTTHVNLHTAPGVGNAMGAIFNAQANHSPLLVTAGQQARA
                     QITLQANLTNRDATRVPHPYVKWSFEPPRAEDVPLALAHGAHLAGLAPRGPVFVSIPM
                     DDWDAEVDEAAARAAIERKVTGRATADPEAVRALAAALDGAANPVLVAGPDVDTSGAW
                     ELAVQLAERQHLPVWASPATGGVRLGFPENHPNYRGVLPPAIGPVGQTLEGHDLIVVV
                     GSSVFPYYPYIPGPLLPEGAKLVAITSDPDEAVRAPMGDAIVADVGLTLAALLEELPE
                     SSRPVPEPNQGPGEIPATDPLNASTVHSALAEVLP"
BASE COUNT         1070 a         2923 c         2829 g         1261 t
ORIGIN      
        1 aggctttgcg ctcggcctcg ctgccgtgca tcccggccgc gttcagcgcc tggatcacgg
       61 cgccggtcgt gtcggtgtcg accagcccgc cgtcgccgcc ggcgccccag ccccagccgc
      121 cgtcgctgcg ctggttgtcg agcagccact gcgccgcccg ccggacgctt tcctcggccc
      181 agcgggcgtc gatcgtgctg agcgggaaga tcgaccacac ggtcgcgttg acccagccct
      241 gtttctggcc cggtttctgg gtgaaggagc cgtccgggag ctgacgctcg agcatccgct
      301 cgatcggccg cacacgcccg aactcgaacg gcgaggtgtc ggaggcgatc gcgaccagcg
      361 ccacccggtc gaagtcggtg gtctgttcga gcccgtcggt gttgcgggtc aggtaggtgt
      421 agacgtcgac gccgccttgc tgcgactggt cctgcgggtt gatcccggcg gcggcgagcg
      481 cgtaggcggc ccaggagctg aagccggggt cggaggcgcc gccgacctca ccgccgaagc
      541 cgccgtcgag gttctgggct tcctgcagga agcgaaccgc cctgtcgagg gttcctttcg
      601 cttcctcgtc gggcgcggcg gagacggccc aggtggggag cagggcgccg gcgagcgcac
      661 aggcggcgag ggcccagagg acccactgcc gcatcgctcg ccggcgccgg gcgctcatcc
      721 gccgccgctc agcttcagcg tcagcgcgcg cgtcttcgtg cccggctcac cgccgctcgg
      781 cgtgaactgc agccgcaccg agaccgccag cctgcccttc gcgagcagct ccgcccgggc
      841 tttcgtcttc ggcttgacca ggacggtgac gaccttggcc gcggtggcgc tgagccgctg
      901 ggtcgcgacc ttggccccgg aggcgaggag ctggccggct cccggcagtg ccaccttcag
      961 cgtcgccgtg cccttgccgc ggttcagggt cagcttgcgc agcccgatca ggttcgaggg
     1021 cttcggcggg tcctggaccg ggggcggggg cgtgccgccg ccgcccgggt gctgcggttc
     1081 ttcttcgtct tcgaggtagc gcggcggcgt gcgcacgagg ctgtcggcgg gcggggtgcc
     1141 gcaactgccg cccccaaccg gttcgacgca gacgtcgagc cggttggagc gcaggaaccc
     1201 ggcttctttc tgggccttga tccggtgcca gcccggggtc gagaaggtga tgctggcctc
     1261 gccttcggcg tcggtgacga ccgccgccga gctgccggtt tcgaccgtct ggtacccggt
     1321 cccttcggcg gtcgcgaccg gggcgatcgt gatcccttcg gcagccgccg ggctcggctg
     1381 ttcgcctttc tcgaccacga gctcgagcgg ctcgccggct tcgacgctgg cggtcggcgc
     1441 ggccgggaag ccgaggtcgc cggccgccga gagccagagc agttggcgac ccatgaaggc
     1501 gtcgtagacc cagaagactt cgtcgccttc ctccgccagg tactggcagc cgccgaccgg
     1561 cgtgaacgcc ccgttgacca ggatgcccca gtacgcgaac gcttcctcgt cctcggcgtc
     1621 cggcccccag cgctggatga agtagtcgtc gaagccgggg taccactcgc cgttgaaggt
     1681 ctggccgatc agttccatcg cgtcgaccga ggccgcggtc ggcgtcggcc cggggtccgg
     1741 gtgggcgccg ttgttggtcg cgtcgcagcg ccgcggttcg gcgtcgctgg tcgcctgcac
     1801 gtcgtgtcct tcggtggcga tcggtccctc gaagagggtc ttctcggccc cctcgatccg
     1861 gacggtcaac tcggtcagag ccgcctgggc tcccgtactc cagataagcg cgacgccaaa
     1921 cacagacagc gccgcagcga tagctcgctt gcgcaacaca acccctttcc tcgagggcag
     1981 atgtcgtacg gaccggaggg aggtctcctg gcttccgagc tatctcgctg cgccttcccg
     2041 gcctctccgg ccagtggctg cgacgtgcgc ttgcaacgag cttcctcggt cacagtggcg
     2101 ggaccgcgcc ggtttttcac cggacttccc tgtccaccga tcctcggttt tgcggggcag
     2161 tgtaacccca ccgtcaaaac gcccggacgc agttgctccc ggcatgcggg aacaactgcg
     2221 tccgggatac ggctattcgg cgtaaaggtg gatcgtgtcg ccgacgttga tcgccgtgct
     2281 gcgtttggcc gtggccgcgg cgccgccgtc gatcgagact ttccattttt cggccggcac
     2341 cgcggggtag ccgttcactt gggtgatcgc gccttcgccg ctggcgggca ggaagccgct
     2401 gaggcagccg gccggcgagc tgccggcgac cgcggcgttc agcaccgtcg ccaggttggt
     2461 cgtcgtcgct ttcggcgcca gcgtcaccga gcagaccttc agcgccgaga cgccgtcgtc
     2521 gactatcagc gccagcgagg tcgtttcggc ttcgccggtg gcgaactggg tgacgccctt
     2581 ccactgcgcg gccccgccgg acgggaccgg gggagccgcg gtgaagccac cgctgccgag
     2641 cgcccgcagg gcgtcctggg tcgcgtagag gttggcggtc gagccgctgg tcccgtagcg
     2701 gaaggcgccg ctccccaact ggttggagat gaggaagttg agcggcgttt ttttgttcgg
     2761 gctcggcccg gtgaattcgg caccctgcgg atcgatcccg cacgctttca ggccctggac
     2821 gacccaggcg ttggagtcgg tgttgttgcc gaattcattt tcgaacgccc cgctcgaccc
     2881 ttcgaagatc gagacgaggt atcccttcgc cgcttcgatc gtcgcgttgg ttttcggcag
     2941 gcccgccccg cagagcgccg cgatcgccgc cccggtcatg tccggttcgg agctcgccgc
     3001 ttcggcttcc gggttgccgg cgacgatcga ccaggtccag ccgccgtcgt cgtgctggtt
     3061 ggcgcggacc gcttcgaccg cgtcgtcgag caccgcctgc ggcacccgga cgactccgcc
     3121 ggtcgttttc acgttggcca gggtgagcag cccgaacacg gtcgcgttca gggtcgagcc
     3181 gtagtagccg ggcgtttccg gctggtagta ggaggcgacc ttggcgatca ggttctgccg
     3241 tttggagacg cgcgccgggt cgatgccggc ggcgtaggcg agcagcgcgg cgcgctcgta
     3301 gtcggtcgcg gtcgcgcctt cgccgggcca gccggcgggg ccgacttcgg cttcgtacca
     3361 actgcgcgcg tcggtggtcg ctttgccgcc tttgttgacg tcggccgccg cgactttggt
     3421 cgccgcgaac gcggtcagag cccagtcgcc gccgaacccg gggatcgccc ccgtttcttt
     3481 gttctgcagc cctttgacgt aggtcagccc tttgccgatc gagccggtga tttcttccgc
     3541 ggtcgcggtc gccagtgccg aggacggcac cagcagtgcg accagggcgg cggcgagcag
     3601 ggcaagccgc caggtcgtgt gacgcatgaa tggatctcct tctggtttgg ggacggtcgt
     3661 tctgccgccc ccaaccacgg gagtgcgggt ggggaggtag accggccgtc cgttcgcgaa
     3721 acaggtggtc cttatgtctg cgtcaggcgg tgagcattcg gactcgggtc gggaggtcgg
     3781 gcctccaccc atcaccgttg cgcgtcagtc ccggattcgc accgggttcc ctcagccgct
     3841 tgacggtggt caaggtagac gggcgggcgg cggcagccgc gccatcccga tctccgctag
     3901 gttgccgctc cacatatcca cggatcggtg gacagggaag ccggtgagat accggcgcgg
     3961 gcccgccact gtgaccgagc aaatacccgc cgcacgccgc acgcggcagc cactggccag
     4021 accggccggg aaggcgcgac gggacaggct cggaagccag gagacctccc tccgatccct
     4081 ccatcagccc tcgaggaaag gggtccaagg atgcgagcag ctcgcgctgt actgctgtcc
     4141 ctctgtctgt cgttgatcgt cctgccggcc gtagcccaag ccgccagcca gaccgaaatc
     4201 gacgccgcga tcgacaaagc cgtcgcctac gccgccagca agcaggaccc ggcgaccggc
     4261 gcgatcgaag acttcggcgg cgactggatg gtgaccgcgc tgaccgcggc cggcgtcgac
     4321 gccggcgact tccgggtcga agcgggtgac ccgagcctgc aggactccta cctcggcgaa
     4381 tacacgctgc cgacctggac cgaagacccg cccagcggca ccgtcaacga gtactcgcgc
     4441 gcagtgctgt tcgcctacgc ggccgggctc gacccggcgc ggatctcggc cgactccaac
     4501 cagccggcgc agctggccgg gcgctggaac ccgaaaaccg gcagcttcgg cgaaaacagc
     4561 tccaacaacg ccgccttcgc gatcctcgcg atgcggaccg ccgggctgcc gagctgggcg
     4621 cttgccccga ccgtcgactt cctgcgccgc aaccagcacg acgacggtgg ctggaccttc
     4681 accgcctcgc tcgaccccgc ttcccaggct gaaccgagcg aagccgacat gaccggcgcc
     4741 gcggtcgcgg cgctctgcga ggccggcgtg ccgacctacg accccgacgt cgccgcggcg
     4801 ctcacctacc tgcaggaact ggtgatcagc ggcacgggcg ggatcgaata catctggggc
     4861 ccgcccaact ccgacgtcaa cgcctgggtg atcagcggcc tgcacgcctg cgggctcgac
     4921 ccgcagggcg ccgagtggac cgtcggcggc aagaacccgg tcgacttcct gctcaccctg
     4981 caggtgcccg agcccgcaga gggcgcgggc ggcttcggct acatggacac ctccgaagcg
     5041 agcctctact cgacccagga cgcactgcgc gcgctcgccg gagccgcctt cacggcggat
     5101 ccgcagagcg tgctcgcgcc tccgagcgtc gccgccggca cccccgtccc gcacctgctg
     5161 gcgatcgagc tggcgcccgg caacgtgcgc ctctgcaagg tgaccgcgcc ggccggcgcg
     5221 ccgctgaccg acctgctcga ggtggcggaa accgcctcgc gcccggccgg ctgcgtccac
     5281 tcgttcgcgg ttgacggagg caaggtgacc gcgatcgacg gcgtcacccc ggacggcgcc
     5341 gacgaggcct ggctggtgcg gctcgaccgc ggcgccgccg ccgcggccgg cgagcagccg
     5401 gtcggtttcg gcgacctggt cacgctgcgc ctcggcagga acccgagcag cgagcagggg
     5461 ccggccggtc ccaccggtcc cgccggtgcg tccggcccgg ttggcgccaa gggcgagccc
     5521 ggaccgcgcg gcaagcgcgg cgcccgcggc aagcccggcc gcaacgcgac gcttgcctgc
     5581 aagcagcggc gcagccgcgc cggcaagccc aaggtccgct gcaaggtggt gcgcgctaag
     5641 cgctaagagt ccgggagcca gccgccttgg cgcccggcgt cgaacccttc tttgtagacc
     5701 gcgagaaagg cgggggagag gggttcgccg ccgggcgcga agcgcgccgc gacgaccagc
     5761 cggcggatca ggtcggcgtg gatgccttcg gtcttctcgg cgccccgttc ggcggcgccg
     5821 atcaggtagc cgagctgcag ggtcgccttg ttgcccgggt cctggcgcgc ttcggcgttc
     5881 agttccgttg ccacctcgat catcgcttcg ccgacgatcg cgagctggcc gccgtcctgg
     5941 ttctcgctga ggcagtcctc gatcggcgtc gtgccttcga gctgcaccgc cgcgggtgcc
     6001 ttcgccagcg cctgcttgta ggcggcggcc ccttccaggc aggcgaccgg cgtgtcttcc
     6061 tcgcgcccgc cgccgcagcc ggcgacgaga agcgccgcgg caattgccgc caggcaggcg
     6121 caggtcaggc tccgggcacg cactcgtcca atcctaggga cgcttccggg tttggtgccc
     6181 ccataggggc ctcaagtccg ggagggctca gtcgccggcg ccgatgccgc cggtcttgta
     6241 ctgcgagatg ccgccggtcg gtccggcggg ctgcttcggc gctttcttcg ccttcttgcc
     6301 cttcggctgc ttggcggtcg ccgagcccgg gggcgagaag acttcgaccc actgggcgac
     6361 ttcgtactgt ttcgccgacg cgtggttcca ggagaggtgc aggtggtggc cgcagccgtg
     6421 gccgctgtcg ccgtcgtagc cgacccagcg gaacggcagc accggtttgt tctggaccgg
     6481 ctcggcccag cgcgcgagcc ggctgatcgc ggtccatttc gcgtcgcagc gggagctcgg
     6541 ttcggccggc acgatgtcga cgccgagccc gttgtagtgg tcggagccgc gcacgtggca
     6601 gccgtggcag cctgcccagc cgccgccggg cgccggcccc gagtagccgt cggtgacgta
     6661 gatcccgtag cgctgggcga gccacttgag gtcggggatg atccgcgagt cgaccatgtc
     6721 gcccgcttcg tgcgggatcg agttcgggat cgggacgatt ttgccgccgt atgcaagccg
     6781 cgtttcgctc ggcggcgctt cggccgcgtc ggcggccgcg gtgcccacgc cgaagcccag
     6841 cgtcaggcag agcagcgcgg tccaggcggt ggcaaagaag atcctcaccc tggggctatc
     6901 ggcagccccc gggcgccctt gaattgcggg gcgatcaatg ctcagtggtt ctccgtgcgg
     6961 gtaggctgct gcccgatgac tacagtgaga gatgcaacgt tcgacctctt ccgcgcgcac
     7021 gggatgacga cgatgttcgg caacccgggc tctaccgagt tgccgatgct gaaggactac
     7081 ccgtccgact tcagctacgt gctggggctg caggaggcgg tcgtggtcgg gatggccgac
     7141 ggcttcgccc aggcctccgg caagaccacg cacgtcaacc tccacaccgc gcccggggtc
     7201 ggcaacgcga tgggggcgat cttcaacgcc caggccaacc actcgccgct gctggtcacc
     7261 gccggccaac aggcccgcgc ccagatcacc ctgcaggcca acctcaccaa ccgcgacgcg
     7321 acccgcgtcc cgcaccccta cgtcaagtgg tccttcgagc cgccgcgcgc cgaggacgtg
     7381 ccgctggcgc tcgcccacgg cgcccacctc gccggactcg ccccgcgcgg accggtcttc
     7441 gtctcgatcc cgatggacga ctgggacgcc gaggtcgacg aggccgccgc ccgcgccgcg
     7501 atcgagcgca aggtgaccgg ccgcgcgacc gccgaccctg aggcggtgcg ggcgctcgcc
     7561 gccgccctcg acggcgccgc caacccggtc ctcgtcgccg gtcccgacgt cgacaccagc
     7621 ggcgcctggg agctcgccgt gcagctggcc gagcgccagc acctgccggt ctgggcctcg
     7681 cccgcgaccg gcggcgtccg cctcggcttc ccggagaacc acccgaacta ccgcggcgtg
     7741 ctgccgccgg cgatcggccc ggtcgggcag acgctcgagg gccacgacct gatcgtcgtc
     7801 gtcggcagct cggtcttccc ctactacccg tacatccccg ggccgctgct gcccgagggg
     7861 gcgaagctgg tcgcgatcac cagcgacccg gatgaggcgg tccgggcacc gatgggggac
     7921 gcgatcgtcg ccgacgtagg cctcaccctg gccgcgctcc tcgaggagct gccggagtcg
     7981 agccgtccgg tcccggagcc caaccagggc cccggcgaaa tccccgccac cgacccgctc
     8041 aacgcctcga ccgtgcacag cgccctcgcc gaggtcctgc ccg
//
LOCUS       DASRIG010000093         5872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_47908, whole genome shotgun sequence.
ACCESSION   DASRIG010000093 DASRIG010000000
VERSION     DASRIG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47908"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFB52_02540"
     CDS             complement(<1..359)
                     /locus_tag="VFB52_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZO05236.1"
                     /translation="MAARPRTLPAAVAPVLVGTAAAVEWAGELPRVGAFIAALIGSIF
                     IQIGTNLANDYSDARRGADTADRLGPVRVTSSGLVTPQRVLRATWIAFAVAVACGIYL
                     TVVAGIVILLIGALSIAA"
     gene            complement(371..1237)
                     /gene="menB"
                     /locus_tag="VFB52_02545"
     CDS             complement(371..1237)
                     /gene="menB"
                     /locus_tag="VFB52_02545"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HZO05237.1"
                     /translation="MPKRHKDAPPEETLYAPIAWQSGGEFEDIRYETAEGIAKITIDR
                     PDVLNAFRPQTLIDVSAAMEMAREDPEVGVIIFTGAGERAFCSGGDQSVRGSTGYVAE
                     GASVGRFHVTDLQIQMRRLPKPIVAMVAGYAIGGGHILHLCCDLTLAADNAVFGQTGP
                     RVGSWDGGYGASLLRDLVGTKKAKEFWLLCRQYDAEEAVEMGLVNAVVPLADLEKETV
                     AWCRDMLHLSPFSLRLVKASFNAHEDGYAGIQQLAHDANLLFYGSEEAAEGREAFKER
                     REPDFSQFPRRP"
     gene            1259..2587
                     /gene="menE"
                     /locus_tag="VFB52_02550"
     CDS             1259..2587
                     /gene="menE"
                     /locus_tag="VFB52_02550"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HZO05238.1"
                     /translation="MKLDDWLAQRSQSCPDRTALIADGDEVTYAQLEAEATWVARRLY
                     AHGVRRGSTAALTMHGRREQVVLAHALMKVGATLLPLNPRLTDAEREQIVAAEEPIAD
                     LDDPGLLTQTEADMPLLGEHDMDDVCARVLTSGSSGTPRPVGLTYGNFLWNAVGSGFN
                     IGVDPADRWLCCVPLSHIAGLGIVMRSVIYGTAAVIHDGFDVDRVAASLEEDGITIVS
                     LVTTMLTRLLEAGVDLSGPRAILVGGGPVPQEPLEEAIARGATVVQTYGLTETCSQVT
                     TLAPADSRRKLGSAGRPLLTTHLRIAADGEILIQGPTVAPGRADADGWLHTGDLGEID
                     DEGFLYVRDRLDDLIVSGGENVVPAEVEEVLLRHPEVADAAVVGREDPEWQQAVTAVV
                     VLESGSQVTPDDLRRHCAESLAGFKVPKQVELVAVLPRTPSGKLMRRALR"
     gene            2599..3603
                     /gene="ilvC"
                     /locus_tag="VFB52_02555"
     CDS             2599..3603
                     /gene="ilvC"
                     /locus_tag="VFB52_02555"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZO05239.1"
                     /translation="MKTDDIFYDKDADLGKLDGKTVAILGYGSQGHAHALNLKDSGAN
                     VVVGLRSDSSSRPKAEGQGFEVLEIADAASRGDIVMILLPDEKQGEVWRNEIADGIAP
                     GNLLLFGHGFAIHFGEIEPPAGVDVGMVAPKGPGHLVRRQFEEGAGVPDLIAVHQDAS
                     GEARDLILAYASGIGGGRAAIIETTFKDECETDLFGEQAVLCGGASELVQAGYETLVE
                     AGYDPRLAYFECLHELKLIVDLMYEKGIQGMHYSISNTAEYGDLTRGKRVITDETKAA
                     MKQILAEIQSGEFAREWIAENRAGGENFDRMRKELEGKEVESVGKELRDMMPWINQEF
                     "
     gene            complement(4069..4488)
                     /locus_tag="VFB52_02560"
     CDS             complement(4069..4488)
                     /locus_tag="VFB52_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO05240.1"
                     /translation="MSAQGESSVTTGDGYAVAHLDDLGQGPGFRKIRKALGVTAFGVN
                     AIVLPPAYQTGFHYHDEQEELYFLHSGRVEIEFGDGSSRELGPGGLAWVDAPTPRRLR
                     NLSDSDEAVYICIGGKDGYVGRDGQELDGPARPEPLS"
     gene            complement(4499..5143)
                     /locus_tag="VFB52_02565"
     CDS             complement(4499..5143)
                     /locus_tag="VFB52_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZO05241.1"
                     /translation="MYPISYEADFNPEPNRWTTFFRILLAIPWLVVGIFWAILFVFTH
                     LIAWVAIIVLGRYPQWLYDFNSGVIRYGIRTSSWVVLQTDVWPPFGISDDPAYPIRVN
                     VPPPAERQSRLKALFRPILALPIIVIVAIVDYIFRFIGLAAWLTIVFRGYLPEWLFST
                     LAHVHSLETRAYGYVAFLTDDYPPVGPERATSAAAPAGDVVPPPPPPPPPAQTA"
     gene            5245..>5872
                     /locus_tag="VFB52_02570"
     CDS             5245..>5872
                     /locus_tag="VFB52_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZO05242.1"
                     /translation="MTVQTDAQIPADLKPADGRFGCGPSKVRPEALARVAESAELMGT
                     SHRQAPVRDLVKGIREGLGELFSLPDGYEVVLGNGGTTAFWESACAWLVRERSLNLVN
                     GEFSSKFAKATAAAPFLADPILVEAEPGDAPDPVADPAADVLGWAHNETSTGVMIDVV
                     RPEGAGDSLVLIDATSGAGGLPVEIAQADAYYFAPQKAFGADGGLWLAV"
BASE COUNT          916 a         2061 c         2029 g          866 t
ORIGIN      
        1 gcggcgatcg agagcgcgcc gatcagcagg atcacgatcc cggcgaccac ggtcaggtag
       61 atcccgcagg cgaccgcgac cgcaaacgcg atccaggtcg cgcgcagcac ccgctgcggc
      121 gtcaccaggc cgctggaggt gacccgcacc ggtcccagcc ggtcggcggt gtcagcgccc
      181 cgtcgggcat ctgagtagtc gttcgcgaga ttcgtcccga tctggatgaa gatcgagccg
      241 atcagcgcgg cgatgaaggc gccgacccgg ggcagctcgc ccgcccactc gaccgccgcc
      301 gcggtcccga ccaggaccgg ggcgaccgcg gccggcaacg tgcgcggccg cgctgccatc
      361 aaccagatcc tcatgggcgg cgggggaact gcgagaagtc gggttcgcgg cgctccttga
      421 acgcctcgcg cccctcggcc gcctcctcgc tgccgtagaa gaggaggttg gcgtcgtggg
      481 cgagctgctg gatcccggcg tagccgtcct cgtgggcgtt gaagctggcc ttgaccaggc
      541 gcagcgagaa gggggagagg tggagcatgt cgcggcacca ggcgaccgtc tccttctcga
      601 gatcggcgag cggcacgacc gcgttgacga gccccatctc aaccgcctcc tcggcgtcgt
      661 actggcggca gagcagccag aactccttcg ccttcttggt cccgaccagg tcgcgcagca
      721 gcgaggcacc gtagccgccg tcccaggagc cgacccgcgg cccggtctgg ccgaagacgg
      781 cgttgtcggc ggccagcgtg aggtcgcagc agaggtggag gatgtggccg ccgccgatcg
      841 cgtagccggc gaccatcgcc acgatcggct tcggcagccg ccgcatctgg atctgcaggt
      901 cggtgacgtg gaagcgcccg acggaggcac cctcggcgac atacccggtc gagccgcgca
      961 cgctctggtc gccgcccgag cagaaggcgc gctcaccggc gccggtgaag atgatcaccc
     1021 cgacctcggg gtcctcgcgg gccatctcca tcgcggcgga cacgtcgatc agcgtctgcg
     1081 ggcggaaggc gttgagaacg tcgggccggt cgatcgtgat ctttgcgatc ccctcggcgg
     1141 tttcgtagcg gatgtcttcg aactcgccgc cgctctgcca tgcgattggc gcgtagaggg
     1201 tctcctcggg cggggcgtcc ttgtggcgtt tgggcatgat ggggacgata tccgcccgat
     1261 gaagctcgac gactggctcg cccagagatc ccagagttgc cccgaccgca cggcgctgat
     1321 cgccgacggc gacgaggtca cctacgccca gctcgaggcg gaagcgacct gggtcgcgcg
     1381 ccggctctac gcccacggcg tgcgccgcgg ctcgaccgcg gcgctgacca tgcacggccg
     1441 ccgcgagcag gtcgtgctcg cccacgcgct gatgaaggtg ggggcgaccc tgctgccgct
     1501 gaacccgcgc ctgaccgatg ccgagcgcga gcagatcgtc gccgccgagg agccgatcgc
     1561 cgacctcgac gacccgggcc tgctcaccca gaccgaagcc gacatgccgc tgcttggcga
     1621 gcacgacatg gacgacgtct gcgcccgggt gctgaccagc ggcagctccg gcaccccgcg
     1681 cccggtcggc ctcacctacg gcaacttcct ctggaacgcg gtcggctccg gcttcaacat
     1741 cggcgtcgac cccgccgacc gctggctctg ctgcgtgccg ctcagccaca tcgccgggct
     1801 cgggatcgtc atgcgctctg tgatctacgg caccgccgcg gtgatccacg acggcttcga
     1861 cgtcgaccgg gtggcggctt cgctggagga ggacgggatc acgatcgtct cgctggtgac
     1921 gacgatgctg acccggctgc tcgaggccgg ggtggacctc tcggggccgc gggcgatcct
     1981 ggtcggcggc ggcccggtcc cgcaggagcc gctcgaagag gcgatcgcca ggggcgccac
     2041 ggtcgtgcag acctacggcc tcaccgagac ctgctcgcag gtgacgaccc tggcgccggc
     2101 cgactcgcgg cgcaagctcg gctccgccgg gcggccgctg ctcaccaccc acctgcggat
     2161 cgccgccgac ggcgagatcc tgatccaggg cccgaccgtc gctcccgggc gcgccgacgc
     2221 cgacggctgg ctgcacaccg gcgacctcgg cgaaatcgac gacgagggct tcctctacgt
     2281 ccgcgaccgg ctcgacgacc tgatcgtcag cggcggcgag aacgtcgtcc cggccgaggt
     2341 cgaggaggtg ctgctgcgcc acccggaggt cgccgacgcc gccgtggtcg gccgcgagga
     2401 tcccgagtgg cagcaggcgg tgaccgcggt cgtcgtgctc gaaagcggct cccaggtgac
     2461 ccccgacgac ctccgtcgcc actgcgccga gtcgttggcc gggttcaagg tccccaagca
     2521 ggtggagctg gtggcggtcc tgccgcgcac cccctcgggc aagctgatga ggcgagcgct
     2581 caggtagcct gctcggccat gaaaaccgac gacatcttct acgacaaaga cgccgacctc
     2641 ggcaagctcg acggcaagac cgtcgcgatc ctcggctacg gctcccaggg gcacgcgcac
     2701 gccctcaacc tcaaggactc cggcgccaat gtggtggtgg gcctgcgatc ggactcctcc
     2761 agtcgcccga aggccgaagg gcagggcttc gaggtcctcg agatcgccga cgcggccagc
     2821 cgcggcgaca tcgtgatgat cctcctgccc gacgagaaac agggcgaggt ctggcgcaac
     2881 gagatcgccg acgggatcgc ccccggcaac ctcctgctct tcggccacgg cttcgcgatc
     2941 cacttcggcg agatcgagcc gccggccggc gtcgacgtcg gcatggtcgc gcccaagggt
     3001 cccggccacc tggtccgccg ccagttcgag gaaggcgccg gcgtgcccga cctgatcgcc
     3061 gtccaccagg acgcgagcgg cgaggcccgc gacctgatcc tcgcctacgc ctccgggatc
     3121 ggcggcggtc gcgccgcgat catcgagacg accttcaaag acgagtgcga gaccgacctc
     3181 ttcggcgagc aggccgtgct ctgcggcggc gcctccgagc tggtccaggc cggttacgag
     3241 accctggtgg aggccggcta cgacccgcgt ctcgcctact tcgagtgcct gcacgagctc
     3301 aagctgatcg tcgacctgat gtacgagaag ggcatccagg ggatgcacta ctcgatctcc
     3361 aacaccgccg agtacggcga cctcacccgc ggcaaacggg tgatcaccga cgagaccaag
     3421 gcggcgatga agcagatcct cgccgagatc cagtcgggcg agttcgcccg cgagtggatc
     3481 gccgagaacc gcgccggcgg cgagaacttc gaccgcatgc gcaaagagct cgagggcaag
     3541 gaagtcgaga gcgtcggcaa agagctgcgc gacatgatgc cctggatcaa ccaggagttc
     3601 tgaggcttag cccggggagg ggtgctgatc ccctccccgt gaattcagtc gcccccctgg
     3661 cggggtggct cccccagatt gagcccgcct atggcgggct tttcattaga ggacagcctt
     3721 cggcacattc ccggggaggg gatcagcacc cctcctcaca cgagccctcg aacttcgcag
     3781 cgcccgctac ccggttcgcc gccgtggctt cgtggcgctc ccgcttggaa gcatcggctg
     3841 cgcctcgcgg agttcgggat ggctggtccc tgatctccaa tgtcctgagt gggactgttg
     3901 gtggcgggga ccgataggtc tctgggcgaa tgtggcccga agggccccga tggtatctgt
     3961 ctctgggccc gccataggcg ggctcctcat tcgggagcta cgacctttag gtcgtgcgac
     4021 tcaattcgcc cagaggccta ccggtccccg cccccgccgg gcccctcgtc aggacaatgg
     4081 ctcgggccga gccggcccat ccagctcttg cccgtcacgg ccgacgtacc cgtctttgcc
     4141 gccgatgcag atgtagacgg cctcgtctga atcgctgaga ttccgcagtc gccgcggcgt
     4201 cggggcgtcg acccaggcga ggccgccggg gccgagctcg cgggacgagc cgtcgccgaa
     4261 ctcgatttcc acgcggccgc tgtgcaggaa gtacagctcc tcctgctcgt cgtggtagtg
     4321 gaacccggtt tggtaggccg gcggcagcac gatcgcgttg acgccgaagg cggtcactcc
     4381 cagtgccttg cggatcttgc ggaaccccgg gccctggccg aggtcgtcga ggtgggcgac
     4441 ggcgtagccg tcgcccgtgg tcacggaact ctccccctgt gcgctcatcg cgaccagttt
     4501 aggccgtctg agcgggtggc gggggcggcg gcggtggggg tacgacgtcc ccggccggtg
     4561 cggctgccga cgttgctctc tctggcccca ccggcgggta gtcatcggtg aggaacgcaa
     4621 cgtagccata ggcccgggtc tcgaggctgt ggacgtgcgc cagggtgctg aacagccact
     4681 ccggcaggta gccgcggaag acgatcgtca gccaggccgc gagcccgatg aagcggaaga
     4741 tgtagtccac gatcgcgacg atgacgatga tcggcagcgc caagatcggg cggaacagcg
     4801 ccttcaggcg actctggcgc tcggccgggg gcgggacgtt gacgcggatt ggatacgcgg
     4861 ggtcgtcact gatcccaaac ggcggccaga cgtcggtctg cagcacgacc caggaggagg
     4921 tgcggatgcc gtagcggatc acccccgagt tgaagtcgta cagccattgc gggtaacgcc
     4981 cgaggacgat gatcgctacc caggcgatca agtgggtgaa gacgaacagg atcgcccaga
     5041 agatgccgac gacgagccac ggaatcgcca aaagaatccg gaagaacgtc gtccagcggt
     5101 tcggttcggg gttgaaatcc gcttcgtagc tgatcggata cacggctcta cctccgttcg
     5161 gactgggaca cacaacggcg ctgtcgagcc taaccccggc cccggcgtca gtccaccgcg
     5221 ccggtcacta aggtgtccgg ctcgatgacc gtccagaccg acgcccagat tcccgccgat
     5281 ttgaagcccg ccgacggccg tttcggctgc ggtccctcga aggtccgccc ggaggccctg
     5341 gcccgcgtcg ccgagagtgc cgagctgatg ggaacctcgc atcgccaggc gccggtccgc
     5401 gacctggtca agggcatccg cgagggcctc ggcgagctct tctcgctgcc cgacggctac
     5461 gaggtcgtgc ttggcaacgg cggcaccacc gccttctggg agtcggcctg cgcctggctc
     5521 gtgcgcgagc gctccctgaa cctggtcaac ggcgagttct cctcgaagtt cgcgaaggcg
     5581 accgccgcgg cacctttcct ggccgacccg atcctggtcg aggccgagcc cggtgacgct
     5641 ccggacccgg tcgccgaccc ggccgccgac gtgctcggct gggcccacaa cgagacctcg
     5701 accggggtga tgatcgacgt cgtccgtccc gagggcgccg gcgactcgct ggtcctgatc
     5761 gacgccacct cgggcgccgg cggcctgccc gtcgagattg cccaggccga cgcctactac
     5821 ttcgcgccgc agaaggcctt cggcgccgac ggcgggctct ggctggccgt gc
//
LOCUS       DASRIG010000094        10064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_568516, whole genome shotgun sequence.
ACCESSION   DASRIG010000094 DASRIG010000000
VERSION     DASRIG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568516"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFB52_02575"
     CDS             <1..416
                     /locus_tag="VFB52_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05243.1"
                     /translation="TAQDGNWDPIETGKKALQLLTKYRNQGGVQGAYGMADYMAAAII
                     QSANQLGIKSGSAPGDLVVTASNCTPTGIPLMEKGELWGNATQSPIEESEAAAARIIE
                     FLEGKEVPHNTTVEEERFTSENYKEFVPLCAEWPN"
     gene            427..1986
                     /locus_tag="VFB52_02580"
     CDS             427..1986
                     /locus_tag="VFB52_02580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO05244.1"
                     /translation="METRTAQKLLEVRHASKSYGAVRALDDASLELAAGEILGLCGHN
                     GAGKSTLVKILTGQVRPDSGEILMQGEPIEMRNSLQAQSHGLAIVDQELSLAPDLSVE
                     ENLFLGNIDLSFLRRPGAIRRRARDLVERIGLDVDPRTPVQRLSIGERQLVEIARLVG
                     RDARVLILDEPTATLSESEIERVFTVARELVREGRSAIFVSHRLDEVLDLCDRVTVLR
                     DGRVVAARSTADIADRQELIKMMIGKELPPPQRSEAVEVAAGSGAVIEALTVPPAVQG
                     FAHEIRGGEIVGLTGQVGSGTSEVLRALAGLEPEARGTLALDGQPVKLGSPTGALRAG
                     IAFASNDRKAEGLFLEKTVEANLVATRLPALSRLGLVRHRALRETARKLADFVKIDAS
                     RLGDTVEHFSGGNQQKVFLGRCLDRGDVKLLILDEPTRGVDVSGRAEIHQLIREAALA
                     GVAVIFASTELDEILELSDVVVTMFGGQIVASLPREQTSAELISADMTMSHGSGKERE
                     AAAPAQEGADR"
     gene            1983..2969
                     /locus_tag="VFB52_02585"
     CDS             1983..2969
                     /locus_tag="VFB52_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05245.1"
                     /translation="MSGAATTADPIAVRDNRATAIQVALWGSLAIFAVLILFGAFTTD
                     GFLTTTNVKAILSSVAFVGMIAVGTALIMISGNLFSLAVGSTAAACAMSFLALLPDGI
                     VLAIVATIALGAVLGLIQGLLVGGIGANPIIVTIGAASLIQGVALWASSGATLVPPKG
                     DTSYEWLNGHILTIPVPVYILFGAVILLDLLLRRSRFGRQLKLVGENREAARAAGLPT
                     TWIITGAFVLAGVFTAIAGVELGAFNESGSLLIEGNLTYDAIAAAVVGGVAITGGRGT
                     VWQALGGAILIAVISDILLLRDYSEGVQILVKGVIVFAVVILTRLNRMRLES"
     gene            2966..4003
                     /locus_tag="VFB52_02590"
     CDS             2966..4003
                     /locus_tag="VFB52_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05246.1"
                     /translation="MSARLPSFFRYRGTTARPFVILAAFAIAFALLPTYASGEVGSAN
                     VFNVFQALSGIGLVALALGLTMIIGEYDLSVSSTYLLGGMLAVKTGGDSPFLGVVVAV
                     AAGLLVGFIQGFLVAKLRMSSMPVTLGGFISLLGLVFLISNSKSVNYSDLSVSIWLND
                     PVMSIFSARSLIAIAIFLIAAAIFAFTWLGRDVRAVGSDREASRTAGVRVDSIIIGTF
                     MSSAAISCFSGALLSYSLAYATPNRTVAPLVFATTMALLGGVSLSGGRGFPLGIAAGA
                     ISLSLLNEGMAVTAAQPYVTYLVTGGLLVVVTIIDAPHLLRWWKTVKARNNIGFRGGS
                     GQLATTKAGDR"
     gene            4000..4791
                     /locus_tag="VFB52_02595"
     CDS             4000..4791
                     /locus_tag="VFB52_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZO05247.1"
                     /translation="MKSEPEQNPEAEDGGDGESVNAVAERQFEAIRHALTERRRAINM
                     TMSELGRIVGVSPSLISQIERGQTLPSVGTLFALAAALGVTVDAFLTEPEPRADAPAP
                     DPENVELGPLPGPDEVLGMPATAREAMYLVRRHARASVPIRGGVTWERLTPRSLEDVE
                     FLELNYAPQAESDDQLYRHPGIEMVLVLEGRFEIHVGFETYLLEEGDSILFPSSLPHR
                     YVNPTDETARAVTVILRDGIGSAIGPEGASSVEDAAPPAPPEQEE"
     gene            4791..5954
                     /locus_tag="VFB52_02600"
     CDS             4791..5954
                     /locus_tag="VFB52_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZO05248.1"
                     /translation="MTTIPTDELERRHAKLRAAAAEAGFDAVLAYSTCKTQANVRWLT
                     GYFTRFAGWQHVQGEEYAMFGSCACLLPVDGEPLLRSDQSWDAPRVEEMSPFADAGST
                     MQLGTDLGRVIAERGYKRVAIDNWHIFPVVHYLALKEAAPDVELVDSRIISEARRVKS
                     PYEIERIRRVEEIADAAAIAGMEATKVGVSEYDAALAAETVARELGDLEAGGNPIIGS
                     TKSFGTGVCLPSRDVVLKSGEWMIFDVLPRFEGYCGDIARMRLAGDESDLDPKLKHYY
                     EATLLINREAIKAIKPGISTGELNDIANEVAEQEGVAEIKSPLLGHATGLDIHDVPDF
                     WNDRSPLQAGEVITIEPCLGIPGEVGTRIEDVILVTEDGYEELTKAPRELVVS"
     gene            5954..6715
                     /locus_tag="VFB52_02605"
     CDS             5954..6715
                     /locus_tag="VFB52_02605"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05249.1"
                     /translation="MKLEGRVALVAGASRGIGESMVRAFSDEGARVLFCARREELGKA
                     IEQDLRERGREVKFVRADVTDEEAVKMLITTAVEVYGGLDVLVTCAGIAPAAPIESME
                     LADWEEVMRINVASMFLLTKHAIPALRKSEHASVITLGSTFGTVGAAGSAAYAVSKAA
                     AINFAKTAALELAEDGIRVNALCPGGTATEFLNEWFESTGDAAGTEQWLREHHPIGRL
                     GEPGDQAKAALFLASDDSSFVTGHGLLVDGGYTAQ"
     gene            6712..7599
                     /locus_tag="VFB52_02610"
     CDS             6712..7599
                     /locus_tag="VFB52_02610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05250.1"
                     /translation="MSAERPVVAFLGTGIMGAAMARNAARAGLTVRGWSVPLSDAERL
                     ADDGIEAFPTAAAASAGADLVVTVVPDAAAIESFATGEDGFLPALGPEAIWIQCSTVG
                     VAPADRLVALAAEHGATLVDSPLLGSKDPAERAELIMLASGEEAAIDRCEPLFEAISR
                     RVVRLGKAGNGSRMKMVTNGWIMTTVAATAEAMALAQALGVPGEGFLEGIAGTALDNG
                     YAQLKGRMILDQDYPAAMRLANGAKDARLVSEATREAGLEGAVISAAAELMARALELG
                     HSEKDMAAAYFAAIEPAQD"
     gene            complement(7618..8505)
                     /locus_tag="VFB52_02615"
     CDS             complement(7618..8505)
                     /locus_tag="VFB52_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05251.1"
                     /translation="MSIDRNAADDFMVTHARPLDRRRFELRAGQTDPAGALAALDGYR
                     NADGGYGWGLEPDLRSPESQPGAARHAFEVFEEIAPATAPQAAALCDWLESVSRPDGG
                     LPFALPLSTPVGSGPWWRDADGSVSSLQITAVTAAIAHRVAVHDPAVASHPWLARATD
                     FCLGAIEALDETPFAYVLAFSIDFLDAVHGARPEADGLLRRLGEHVPGDGRVPVRGGT
                     EDEALRPLDIAPYPGRPARELFDEEVIAADLDRLEAGQHDDGGWSVDYLQISPAGSMD
                     WRGYATVHAIEILDRNGRI"
     gene            complement(8586..9281)
                     /locus_tag="VFB52_02620"
     CDS             complement(8586..9281)
                     /locus_tag="VFB52_02620"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO05252.1"
                     /translation="MDLVPGYRELPAPAALHGTVACLWVRVTADAGEVRVVPDGCTDV
                     IWQRGAGTSMAGPDTGPRLIGCAAGDVLVGMRFRPGVGGGALGVPLAELRDLQVEMAV
                     LDRAFDADGELEPEEVVARFLAAAAGREPDPLVALATPRVLDQQVDSIARDLGISERQ
                     LRRRFHAAAGYGPKTLARVLRFRRFVAAVDAGRTDFAAIAFDAGYADQAHLTREVTRL
                     AGLPPAAFLRVWR"
     gene            complement(9298..>10064)
                     /locus_tag="VFB52_02625"
     CDS             complement(9298..>10064)
                     /locus_tag="VFB52_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO05253.1"
                     /translation="TVDELIAAGPDAWSATAAAAAEALAADAAPLAEAELAPPLRAPS
                     KIVCIGLNYRDHCRETGLDEPERPLVFAKFLSTLAAPGEEIWWPDGLTEQVDWEAELA
                     AVVGRRLRDVSTEEALDGVFGYTAANDVSARDLQFGDVQWVRGKSLDSFCPLGPTLVT
                     ADEYGDPQDKKLFCRVNGETKQESTTAEMIFGVAEIVSFLSHNFTLEPGDLILTGTPW
                     GVGGFADPPVFLQAGDTVEVEVEGIGVLRSPVAGPR"
BASE COUNT         1532 a         3610 c         3504 g         1418 t
ORIGIN      
        1 ccaccgcgca ggacggcaac tgggacccga tcgaaaccgg caaaaaggcg ctccagctgc
       61 tgaccaaata ccgcaaccag ggcggcgtcc agggggccta cggaatggcc gactacatgg
      121 ccgcggcgat catccagtcc gccaaccagc tcgggatcaa aagcggcagc gcccccggcg
      181 acctggtcgt gacggcgagc aactgcacgc cgaccggcat cccgctgatg gaaaaaggcg
      241 aactctgggg caacgccacc cagtcgccga tcgaggagtc cgaagcggcc gccgcgcgga
      301 tcatcgaatt cctcgaaggc aaagaagttc cccacaacac gacggtcgaa gaggaacggt
      361 tcacgagcga gaactacaaa gaattcgttc cgctgtgcgc cgaatggccg aactgagggc
      421 ctgacgatgg agacccgcac cgcgcaaaag ctgctcgaag tgcgccatgc ctcgaagagc
      481 tatggcgcgg tgcgggcact cgacgacgcc tcgttggagc tggccgcagg ggagattctc
      541 ggcctctgcg gccacaacgg ggccggcaag agcaccctgg tgaagatcct caccggccag
      601 gtgcggccgg actccggcga gatcctcatg cagggcgagc cgatcgagat gcgcaactcg
      661 ctgcaggcgc agtcgcacgg gctcgcgatc gtcgaccagg agctcagcct cgcccccgac
      721 ctctcggtcg aggagaacct cttcctcggc aacatcgacc tctccttcct gcgccgaccc
      781 ggcgcgatcc gccgccgcgc ccgcgacctg gtcgagcgaa tcgggctcga cgtcgacccc
      841 cgcaccccgg tgcagcggct ctcgatcggt gagcggcagc tggtcgagat cgcccgcctg
      901 gttggtcgcg acgcccgcgt gctgatcctc gacgagccga ccgcgacgct ctccgagagc
      961 gagatcgagc gcgtcttcac ggtcgcccgc gagctcgtcc gcgaaggccg cagcgcgatc
     1021 ttcgtctcgc accggctcga cgaggtgctc gacctctgcg accgggttac cgtgctgcgc
     1081 gacggccgcg tcgtcgccgc ccgcagcacc gccgacatcg ccgatcgcca ggagctgatc
     1141 aagatgatga tcggcaaaga gcttcctccc ccgcagcgct ccgaagcggt cgaggtggcc
     1201 gccggaagcg gcgccgtgat cgaggccctc accgttcccc ccgcggtgca gggcttcgct
     1261 cacgaaatcc gcggcggcga gatcgtcggc ctcaccggcc aggtcggctc cgggaccagc
     1321 gaggtgctgc gagcgctggc cgggctcgag cccgaggcgc gtggcaccct tgcgctcgac
     1381 ggccagccgg tcaagctcgg ctccccgacc ggcgccctgc gcgccggcat cgccttcgcc
     1441 tccaacgacc gcaaggccga gggcctgttc ctcgaaaaga cggtcgaggc gaacctcgtt
     1501 gcgacccgcc tgccggcgct ctcccgcctc ggcctggtcc gccaccgggc gctccgcgag
     1561 accgcccgca aactcgccga cttcgtcaaa atcgacgcct cgcggctcgg cgatacggtc
     1621 gagcatttca gcggcggcaa ccagcagaaa gtcttcctcg gccgctgcct cgaccgcggc
     1681 gacgtgaagc tgctgatcct cgacgagccg acccgcggcg tcgacgtctc cggccgcgcc
     1741 gagatccacc agctgatccg cgaagcggcg ctggcggggg tagcggtcat ctttgcctcg
     1801 accgagctcg acgagatcct cgagctctcc gacgtcgtgg tgacgatgtt cggcggccag
     1861 atcgtcgcct cgctgccgcg cgagcaaacc agcgcggagc tgatctccgc tgacatgacg
     1921 atgagccacg ggagcggcaa agagcgcgag gcagccgccc cggcccagga aggagcagac
     1981 cgatgagcgg cgcagccacc accgccgacc cgatcgccgt ccgcgacaac cgcgccaccg
     2041 cgatccaggt ggccctctgg ggatcgctcg cgatcttcgc ggtgctgatc ctgttcggcg
     2101 ccttcaccac cgacggcttc ctcaccacca ccaacgtgaa ggcgatcctc tcctcggtcg
     2161 ccttcgtcgg catgatcgcg gtcgggaccg cgctgatcat gatcagcggc aacctcttct
     2221 cgctggcggt cggctcgacc gcggcggcct gcgcgatgtc cttcctcgcc ctgctcccgg
     2281 acgggatcgt gctggcgatc gtcgcgacga tcgcgctcgg cgccgtgctc ggattgatcc
     2341 agggcctcct ggtcggcggg atcggcgcca acccgatcat cgtcacgatc ggcgccgcca
     2401 gcctgatcca gggcgtcgcc ctctgggcca gctccggggc gaccctggtg ccaccgaaag
     2461 gcgacaccag ctacgagtgg ctgaacgggc acatcctcac gatccccgtc ccggtctaca
     2521 tcctcttcgg cgcggtgatc ctgctcgacc tgctgttgcg ccgcagccgc ttcggccggc
     2581 agctgaagct ggtcggcgag aatcgcgagg cggcccgcgc cgccggcctg ccgacgacct
     2641 ggatcatcac cggcgccttc gttctcgccg gcgtcttcac cgcgatcgcc ggggtcgagc
     2701 tgggggcctt caacgaaagc ggctcgctgc tgatcgaagg gaacctcacc tacgacgcga
     2761 tcgccgccgc ggtggtcggc ggggtcgcga tcaccggagg ccgcggcacc gtctggcagg
     2821 cgctcggcgg ggcgatcctg atcgcggtga tctccgacat cctgctcctc cgcgactaca
     2881 gcgaaggggt ccagatcctg gtgaaaggag tgatcgtgtt cgccgttgtg atcctgacgc
     2941 gactcaaccg gatgaggctc gaatcatgag cgcccggctg ccctccttct tccgttaccg
     3001 cggcaccacg gcgcggccgt tcgtgatcct cgcggccttc gcgatcgcct tcgccctgct
     3061 gccgacctac gccagcggcg aagtcggcag cgccaacgtc ttcaacgtct tccaggctct
     3121 ctccggaatc ggcctggtgg cgctggccct gggcctgacg atgatcatcg gcgagtacga
     3181 cctctcggtc tcctcgacct acctgctggg cgggatgctg gcggtcaaga ccggcggcga
     3241 ctcgccgttt ctcggggtcg tggtggcggt cgcggccggc ctcctggtcg ggttcatcca
     3301 gggcttcctg gtcgcgaagc tgaggatgag ctcgatgccg gtgaccctgg gcggattcat
     3361 ctccctgctc gggctcgtct tcctgatcag caactcgaaa agcgtcaact actccgacct
     3421 cagcgtcagc atctggctca acgatccggt gatgtcgatc ttctcggcgc gcagcctgat
     3481 cgcgatcgcg atcttcctga tcgccgccgc gatctttgcc ttcacctggc tgggccgaga
     3541 cgtgcgcgcg gtcggctcgg accgcgaggc gagccgcacc gccggggtcc gggtcgactc
     3601 gatcatcatc ggcaccttca tgtcctcggc ggcgatctcc tgcttctcgg gagcgctgct
     3661 cagctacagc ctcgcctacg cgaccccgaa ccgaaccgtc gccccgctcg tcttcgcgac
     3721 cacgatggcg ctgctcggcg gcgtctcgct ctccggcggc cgcggctttc cgctcgggat
     3781 cgccgccggg gcgatctcgc tcagcctgct caacgagggg atggcggtga ccgccgcgca
     3841 gccctacgtc acctatctgg tgaccggtgg gttgctggtc gtggtaacga tcatcgacgc
     3901 cccccacctg ctgcgctggt ggaagaccgt gaaggcccgc aacaacatcg ggttccgcgg
     3961 tggctcgggg caattggcaa ccacgaaggc gggagacaga tgaagagcga gccggagcag
     4021 aaccccgagg ccgaggacgg cggcgacggc gagagcgtca acgccgtcgc cgagcgccag
     4081 ttcgaggcga tccgccacgc gctgaccgag cggcggcggg cgatcaacat gacgatgtcg
     4141 gagctgggcc ggatcgtcgg cgtctcgccc agcctgatca gccagatcga gcgcggccag
     4201 accctgccgt cggtcggcac cctgttcgcc ctcgcggcgg cgctcggggt caccgtcgac
     4261 gccttcctca ccgagcccga gccccgcgcc gacgcgccgg cgccggaccc cgagaacgtc
     4321 gagctggggc cgctgcccgg gcccgacgag gtgctcggga tgccggcgac ggcccgcgag
     4381 gcgatgtacc tggtgcgtcg ccacgcccgc gccagcgtgc cgatccgcgg cggcgtcacc
     4441 tgggaacgcc tcaccccgcg ctcgctcgag gacgtcgagt tcctcgagct caactacgcg
     4501 ccgcaggccg agtccgacga ccagctctac cgccacccgg gcatcgagat ggtgctcgtg
     4561 ctcgagggca ggttcgaaat ccacgtcggc ttcgagacct acctgctcga ggagggagac
     4621 agcatcctct tcccctcctc gctgccccac cgctacgtca acccaaccga cgagaccgcc
     4681 cgtgccgtga cggtgatcct ccgcgacggc atcggctccg caatcggccc agagggtgcg
     4741 agcagcgtcg aggacgctgc accccccgcg ccacccgaac aggaggagta atgaccacga
     4801 tccccaccga cgagctggag cgccgccacg cgaagctgcg agcggcggcc gccgaggccg
     4861 gcttcgacgc cgtgctcgcc tactcgacct gcaagacgca ggccaacgtg cgctggctga
     4921 ccgggtactt cacccgcttc gccggctggc agcacgtgca gggcgaggag tacgcgatgt
     4981 tcggttcctg cgcctgcctg ctgccggtcg acggcgagcc gctgctgcgc tccgaccaga
     5041 gctgggacgc gccgcgggtc gaggaaatgt cgcccttcgc cgatgccggc tcgaccatgc
     5101 agctcggcac cgacctcggc agggtgatcg ccgagcgcgg ctacaagcgg gtggcgatcg
     5161 acaactggca catcttcccg gtcgtccact acctggcgct gaaggaggcg gctccggacg
     5221 tcgagctggt cgactcgcgg atcatctccg aggcacggcg ggtcaagtcg ccctacgaga
     5281 tcgagcggat ccgcagggtc gaggagatcg ccgacgccgc cgcgatcgcc ggcatggaag
     5341 cgaccaaggt cggggtcagc gagtacgacg cggcgctggc tgcggaaacg gtggcgcgcg
     5401 agctcggcga cctcgaagcg ggcggcaacc cgatcatcgg ctcgaccaag agctttggca
     5461 ccggcgtctg cctgccgagc cgcgacgtcg tcctgaagtc gggcgagtgg atgatcttcg
     5521 acgtcctccc ccgcttcgag ggctactgcg gcgacatcgc ccgcatgcgc ctggccggcg
     5581 acgagtccga cctcgacccg aagctgaagc actactacga ggcgaccctg ctgatcaacc
     5641 gcgaggcgat caaggcgatc aagccgggaa tctcgaccgg cgagctcaac gacatcgcca
     5701 acgaagtcgc cgagcaggag ggcgtcgctg agatcaagag cccgctgctc gggcacgcga
     5761 ccggcctcga catccacgac gtgcccgatt tctggaacga ccgcagcccg ctgcaggccg
     5821 gcgaggtgat cacgatcgag ccctgcctgg ggatccccgg cgaggtcggg acccggatcg
     5881 aggacgtgat cctggtcacc gaggacggct acgaggagct gaccaaggcg ccgcgcgagc
     5941 tggtggtgag ctgatgaagc tcgagggacg ggttgcgctc gtcgccggcg ccagtcgcgg
     6001 aatcggcgag tcgatggtgc gggcgttctc cgacgagggc gcgcgggtgc tcttctgcgc
     6061 ccgccgcgag gagctcggca aggcgatcga gcaggacctg cgagagcgcg gccgcgaggt
     6121 caagttcgtg cgcgccgacg tcaccgacga ggaggcggtg aagatgctga tcaccaccgc
     6181 ggtcgaggtc tacggcggcc tcgacgtgct cgtaacctgc gccgggatcg cccccgccgc
     6241 gccgatcgag tcgatggagc tggccgactg ggaagaggtg atgcgaatca acgtagccag
     6301 catgttcctg ctgaccaagc acgcgatccc ggccctgcgc aaatccgagc acgcctcggt
     6361 gatcacgctc ggctcgacct tcggtacggt cggcgccgcc ggctcggccg cctacgcggt
     6421 ctcgaaagcg gcggcgatca acttcgccaa gacggcggcg ctggaactgg ccgaggacgg
     6481 gatccgggtc aacgcgctct gcccgggcgg gaccgcgacc gagttcctca acgaatggtt
     6541 cgagtcgacc ggggacgccg ccggcaccga gcagtggctg cgcgaacacc acccgatcgg
     6601 ccgcctcggc gagcccggcg accaggccaa ggcggcgctc ttcctcgcct ccgacgactc
     6661 cagcttcgtc accggccacg ggctgctggt cgacggcggc tacacggccc aatgagcgcc
     6721 gagcgtcccg tcgtcgcctt cctcggcacc gggatcatgg gcgcggcgat ggcccgcaac
     6781 gcggcccggg cgggcctcac ggtgcgcggc tggagcgtgc cgctgtccga tgccgagcgg
     6841 ctcgccgacg atgggatcga ggcgttcccg accgccgccg cggcgagcgc cggggccgac
     6901 ctcgtggtca ccgtcgtccc cgacgcggcg gcgatcgaat cgttcgcgac cggtgaggac
     6961 ggcttcctgc cggcgctcgg tcccgaggcg atctggatcc agtgcagcac ggtcggggtg
     7021 gcgccggccg accgcctggt cgcgctcgcc gccgagcacg gggcgacgct cgtcgactcc
     7081 cccctgctcg gctccaagga cccggccgaa cgggccgagc tgatcatgct ggcctccggc
     7141 gaggaagctg cgatcgaccg ctgcgaaccg ctcttcgagg cgatctcccg ccgcgtcgtg
     7201 cggctcggca aggctggcaa cggcagccgc atgaagatgg tgaccaacgg ctggatcatg
     7261 accacggtcg ccgccaccgc cgaggcgatg gccctggccc aggcgctcgg cgtgcccggc
     7321 gagggcttcc tcgaagggat cgccggcacc gccctcgaca acggctacgc ccagctcaag
     7381 ggccggatga tcctcgacca ggactacccg gcggcgatgc gcctcgccaa cggcgccaag
     7441 gacgcccgcc tggtcagcga ggcgacccgc gaagccggcc tcgagggtgc cgtgatctcc
     7501 gccgccgccg agctgatggc ccgcgcgctc gagctcggcc actccgagaa ggacatggcc
     7561 gccgcctact tcgccgcgat cgagcccgcg caggactagc gcgggcgccc cgcggcgcta
     7621 gatccgtcca ttccgatcga ggatttcgat cgcgtggacg gttgcgtatc cgcgccagtc
     7681 catcgatccg gccggggaga tctgcaggta gtcgacgctc cagccgccgt cgtcgtgctg
     7741 cccggcttcg aggcggtcca ggtcagcggc gatcacctcc tcgtcgaaca gctcccgcgc
     7801 cgggcgcccg gggtagggag cgatgtccag ggggcgcagc gcctcgtcct cggtgccgcc
     7861 tcggaccggg acgcggccgt cgccggggac gtgctcgccg agcctgcgca gcaggccgtc
     7921 cgcctccggg cgcgccccgt gcaccgcgtc gaggaagtcg atcgagaagg cgaggacgta
     7981 ggcgaagggc gtctcgtcga gcgcctcgat cgcgcccagg cagaagtcgg tcgcccttgc
     8041 cagccagggg tgggaggcga cggccgggtc gtggacagcg acgcggtggg cgatggccgc
     8101 cgtcaccgcg gtgatctgca gcgaggacac cgaaccatcg gcgtcacgcc accaggggcc
     8161 ggagccgacc ggggtgctca gcggcagggc gaacggcagg ccgccgtcgg ggcgggagac
     8221 cgattccagc cagtcgcaca gcgccgctgc ctgcggggcg gtggcggggg cgatctcctc
     8281 gaacacctcg aaggcgtgcc gcgcggcgcc gggctggctc tcgggcgagc gcagatcggg
     8341 ctcgaggccc cagccgtagc caccgtcggc gttgcgatag ccgtcgagcg cggcgagcgc
     8401 gccggcgggg tcggtctgac cggcgcgcag ctcgaagcgg cggcggtcga gcgggcgggc
     8461 gtgggtaacc atgaagtcgt ccgcggcgtt tcggtcgatg ctcatcgtgg gtcctttcgt
     8521 cgtggtggat ccatggttgc gtccagcgcg cggcctgtct tgaacaaaac ggacgactcg
     8581 ggcggctatc gccagacgcg caggaaggcc gccggcggca gcccggcgag gcgggtcacc
     8641 tcgcgggtca ggtgcgcctg gtcggcatag ccggcatcga aggcgatcgc cgcgaagtcg
     8701 gtgcgaccgg cgtcgaccgc ggcgacgaac cgcctgaagc gcaggaccct ggccagcgtc
     8761 ttcggcccgt agccggcggc ggcatggaaa cggcggcgca gctggcgctc gctgatcccg
     8821 agatcgcggg cgatcgagtc cacctgctga tcgagcacgc gtggcgtcgc cagcgcgacc
     8881 agcgggtccg gctcccggcc cgccgcggcg gcgagaaacc gcgcgaccac ctcctctggc
     8941 tcgagttcgc cgtcggcgtc gaaggcgcgg tcgagcacag ccatctccac ctgcaggtcg
     9001 cgcagctcgg ccagcgggac cccgagtgcg ccgccgccca ccccgggacg gaagcgcatc
     9061 ccgaccagga cgtcgccggc cgcgcagccg atcagcctcg gcccggtatc gggcccggcc
     9121 atgctcgttc ccgcgccccg ttgccagatc acatccgtgc acccgtcggg gacgacccgc
     9181 acctcgcccg cgtccgcggt cacgcggacc cagaggcagg cgacggtgcc gtgcagcgcc
     9241 gcgggagcag gcagctcgcg gtagccgggg accaagtcca cccggccatg atcgcactca
     9301 gcgcgggccg gctacgggac ttctgagcac gccgatgccc tcgacctcga cctcgacggt
     9361 gtcgccggcc tgcaggaaga cgggcgggtc ggcgaagccg ccgacgcccc acggggtgcc
     9421 ggtgaggatc aggtcgccgg gctcgagggt gaagttgtgg gagaggaagc tgacgatctc
     9481 ggcgacgccg aagatcatct cggcggtcgt cgactcctgc ttggtctcgc cgttgacgcg
     9541 gcagaagagc ttcttgtcct gggggtcgcc gtactcgtcg gccgtaacca aggtcgggcc
     9601 gagggggcag aagctgtcga ggctcttgcc gcgcacccac tggacgtcgc cgaactgcag
     9661 gtcgcgggcg ctgacgtcgt tggcggccgt gtagccgaag acgccgtcga gcgcctcctc
     9721 ggtggagacg tcgcggagcc ggcggccgac cacggcggcc agctcggcct cccagtcgac
     9781 ctgctcggtg aggccgtccg gccaccagat ctcctcgccc ggagccgcca gggtggagag
     9841 gaacttggcg aagacgagcg gccgctcggg ctcgtcgagg ccggtctcgc ggcagtggtc
     9901 gcggtagttg aggccgatgc agacgatctt cgagggcgcc cgcagcgggg gcgccagctc
     9961 cgcctcggcg agcggcgcgg cgtcggcagc gagcgcctcg gcggcggccg ctgccgtcgc
    10021 gctccaggcg tccgggccgg cggcgatcag ctcgtcgacg gtgg
//
LOCUS       DASRIG010000095         4585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2917967, whole genome shotgun sequence.
ACCESSION   DASRIG010000095 DASRIG010000000
VERSION     DASRIG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917967"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFB52_02630"
     CDS             complement(<1..1361)
                     /locus_tag="VFB52_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05254.1"
                     /translation="MLVAAAFGAINVSSAAALGEQCSGKKSRGLGAFLQTQAQQRWAS
                     GEAGFNGSPNPLACSGTQGSGGTPEVSYTPIASPAALHYWGADDGILHSGEFETTFDH
                     LGTDIAPAGPVAEEGTQLANMKAALGSDVVVVPVTQTAIAIVANPPALPAHPACTVPR
                     INAAQLQKVFSGELKNWRQLGAASDSTLGGDCDQAITRIVRDESAGTTYQFKHYLNSV
                     NPAPLACSGKEKRTWAQLQAPFGGETSPNREWPRNADCQEGEGPVTTVAGPGGEGEYN
                     LSYVASTPGTITYGSLPQAQQVASKQIIEVHNGEKPANPANKEGGANCAAAKYAVPAG
                     AKAGLNIDWSQVYGSNPTIGETAKNAYPICTLTWAVAAADSGGLFGTGVATTLRDYLA
                     FVVDNEGGQAVVRNAGYDDLPNPVAKAAVAAIAQIGGEGEEEEEGGSGTVLCKVAPEN
                     VGGV"
     gene            complement(1566..1751)
                     /locus_tag="VFB52_02635"
     CDS             complement(1566..1751)
                     /locus_tag="VFB52_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05255.1"
                     /translation="MERWNDDRMDDLSGRVSHLEGRMDARFDKLESRFDRLQNTLIVT
                     LGALVSAVLTFVAAAQF"
     gene            complement(1869..3827)
                     /locus_tag="VFB52_02640"
     CDS             complement(1869..3827)
                     /locus_tag="VFB52_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05256.1"
                     /translation="MFRRLIYAALLVAAFAALQAPAASALGEQCSGSDIQGGGGYLQY
                     PSQARWSHDKPFGFNGSSSPLACDGSQGAGGKPEMSAYGPWGSAAALHNFGADDGVLH
                     VEGREFIGTDIAPSGPVGEEGSELANVKKALGSDAVVVPVTQTAIAIAANPPALPAHA
                     PCTVTGISSEQLEKVFSGVITNWLQLSTASDKEPGGDCDQAITRVVREEGSGTTYQFK
                     HFLGQVNGSGLACTGESKRTWAQLQPAAGESPPNVRWPRKADCQEGEGPVTTVTGSVG
                     EGAPGPLIYVAANPGTITYAPLPEAQNHASKQIIDVYNGVQFASPSTGENEANCAAAK
                     YTLPEGWEAGLSVDWSQVYGSDPAIGEAAESAYPICTLSWDVTASDAIKAFGKAAATT
                     VRDYLAFVISEAGQAAVQPVWYSPLPGPVAEAAAAAVNQIGGEEPEEPEEEESSPYGT
                     VLCNVEPEVVGQVLDCPVGQRFTGEKIIGGVYPGTTTTFEALGSGPKGTISCAAASLV
                     GDFLEFGQADTGLYVLTFGVSEGCATTLPGEPEAVVKLASPPYNESRFVYLGKLAPQG
                     AFVIASREGWGETQELQLEGVAENCLYTASYLAGQVVNGSPTKLLLQGTWGLTEGSAE
                     ECPETLQQSSQLTLKATNEGLPLYVAFE"
     gene            3976..>4585
                     /locus_tag="VFB52_02645"
     CDS             3976..>4585
                     /locus_tag="VFB52_02645"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO05257.1"
                     /translation="MATTPILLLSLPRSGSTVVQRVLGAHEEVETASESWLLLSLLLS
                     LRDDVPAPGAWQPRIHAALGDFAAELPDGVASYERAAGTAARALYEEAAGGARFYLDK
                     TPPYFLVVDEIVRALPEARIVVLWRNPLAVLASVVETFCGGRWRPQDYPTSLFGGLER
                     LVAAVLRYPSRIHTVRYEDLVGGEHDAWRGLTEFAGFEFDPGS"
BASE COUNT          633 a         1474 c         1622 g          856 t
ORIGIN      
        1 acgccgccga cgttttccgg ggcgactttg cagagcaccg tcccggaccc gccttcttct
       61 tcttcttcgc cttcaccgcc gatctgggcg attgcggcga ccgcggcttt ggcgaccggg
      121 ttcggcaggt cgtcgtagcc agcattgcgg accacagcct gaccgccttc gttgtcgacg
      181 acgaaggcga ggtagtcgcg cagggtcgtc gccacgccag tgccgaacag gccgccggag
      241 tcggccgcgg caacggccca ggtcagggtg cagatcgggt aggcgttttt cgccgtttcg
      301 ccgatcgtcg ggttggagcc gtagacctgc gaccagtcga tgttgagccc ggctttcgcc
      361 ccggcgggca cggcgtattt cgcggcggcg cagttggccc cgccttcttt gttcgctggg
      421 ttggccggtt tttcgccgtt gtggacctcg atgatctgtt tcgaggccac ctgctgcgct
      481 tgcggcaggc tgccgtaggt gatcgtgccg ggggtcgagg cgacgtagga gaggttgtac
      541 tcgccttcgc cgccgggacc ggcgaccgtg gtcacggggc cctcgccttc ctgacagtcg
      601 gcgttgcgcg gccattcccg gttgggcgag gtttcgcccc caaacggagc ctgcagttgc
      661 gcccaggtcc gtttttcttt gccgctgcag gcgagcggtg ccgggttgac cgagttcaga
      721 tagtgcttga actggtaggt ggtgccggcg ctttcgtcgc ggacgatccg ggtgatcgcc
      781 tggtcgcagt cgccgcccag ggtcgagtcg gaagcggcgc cgagctggcg ccagttcttc
      841 agttcgccgc tgaacacttt ctgcagctgc gcggcgttga tccgcggcac ggtgcaagcc
      901 gggtgcgcgg gcagggcggg cgggttggcg acgatcgcga tcgccgtctg ggtcaccggg
      961 acgacaacca cgtcgctgcc cagcgcagct ttcatgttgg ccagctgggt gccttcttcg
     1021 gcgaccgggc ccgcgggcgc gatgtcggtg ccgaggtggt cgaaggtcgt ttcgaattcg
     1081 ccgctgtgca ggatgccgtc gtcggcgccc cagtagtgga gggccgccgg gctggcgatc
     1141 ggcgtgtagc tgacttcggg cgtgccgccg ctgccctggg tgccgctaca ggccagtggg
     1201 ttcggcgagc cgttgaagcc ggcttccccc gacgcccagc gctgctgggc ctgcgtctgc
     1261 aggaacgcgc cgaggccgcg ggattttttc ccggaacact gttcgcccag cgccgcggcg
     1321 ctggacacgt tgatcgcgcc gaacgccgct gcaaccagca cgacgcaggc aacgaggcca
     1381 aagcgccggc atcccgacgc gcgcctggtc cttctcaacc agaacatgta actccccttc
     1441 gtttggggcc gcggaggaca accgcggccg ttggccaccc ctgccagggg cgagctaagt
     1501 cctattaatc aaggggagtc gaagaagttg cgggggcgcg gcaggttggc ccggtccggg
     1561 ccgaatcaga attgggcggc ggcgacgaag gtgagcaccg cggagaccaa ggcaccgagc
     1621 gtgacgatga gagtgttctg gagccggtcg aagcgcgatt cgagtttgtc gaagcgcgca
     1681 tccatgcggc cctccaagtg gctgacgcgg ccggacaggt cgtccatgcg atcgtcgttc
     1741 catcgctcca tcgtggaagg agcgtagcgg acaaaacgtt acaggtggcg catggaagtg
     1801 tgaaatttgg aggggccgcg ccagcgtggc gcggcccctc cgcttgccta gagaccgtct
     1861 gtcgcgcgct attcgaacgc gacgtagagc ggcaggcctt cgttggtcgc tttcagggtc
     1921 aactgcgagg actgctgcag cgtttccggg cattcttccg ccgagccttc ggtcagcccc
     1981 caggtcccct ggaggaggag cttggtcggg ctgccgttga caacctggcc ggcgaggtag
     2041 ctcgccgtgt acaggcagtt ttccgccact ccttcgagct gcagctcctg cgtttcgccc
     2101 cacccttctc tgctcgcgat cacgaacgcg ccctgcggcg cgagttttcc gaggtagacg
     2161 aaacgcgatt cgttgtacgg cggactggcc agtttgacca ccgcttccgg ttcgccgggg
     2221 agggtcgtcg cgcacccttc ggagacgccg aaggtgagca cgtagagacc ggtgtcggcc
     2281 tgcccgaatt cgaggaagtc gccgaccagc gaggcggcgg cgcagctgat cgtccctttc
     2341 gggccactgc cgagtgcctc gaacgtagtc gtcgttcccg ggtacacgcc gccgatgatt
     2401 ttttccccgg tgaagcgctg cccaaccggg cagtcgagaa cctgcccgac gacttccggt
     2461 tcgacgttgc agagcacggt cccgtacggg ctgctttctt cttcttcggg ttcttcgggt
     2521 tcttcgccgc cgatctggtt gaccgcggcc gcggccgctt cggcgaccgg gccgggcagc
     2581 ggcgagtacc agaccggctg caccgcggcc tgccccgctt cggagatgac gaaggcgagg
     2641 tagtcgcgga ccgtggtcgc ggccgctttg ccgaacgctt tgatcgcgtc ggaggcggtc
     2701 acgtcccagc tcagcgtgca gatcgggtaa gccgattccg ctgcttcgcc gatcgccgga
     2761 tcggagccgt agacctgcga ccagtcgacg ctcagccccg cttcccagcc ttccggcagg
     2821 gtgtatttgg cggccgcgca gttggcttcg ttttcgcccg tcgacgggct cgcgaactgg
     2881 acgccgttgt agacgtcgat gatctgtttc gaggcgtgat tctgtgcttc cggcaggggc
     2941 gcgtaggtga tcgtgccggg gttggcggcg acatagatga gcgggccggg cgcgccttcg
     3001 ccgaccgaac cggtcaccgt ggtcaccggc ccttcgcctt cctggcagtc cgctttgcgc
     3061 ggccacctga cgttcggcgg gctttcgccg gcggccggct ggagctgcgc ccaggtccgt
     3121 ttcgattcgc ccgtgcaggc gaggccggaa ccgttgacct ggccgaggaa gtgcttgaac
     3181 tggtaggtgg tgccggagcc ttcttcgcgc acgacgcggg taatcgcctg gtcgcagtcg
     3241 ccaccgggtt ctttgtcgga ggcggtgctg agctgcagcc agttggtgat cacgccgctg
     3301 aacactttct cgagctgttc agagctgatc cccgtcaccg tgcagggcgc gtgggccggc
     3361 agcgcgggtg ggttggccgc aatcgcgatc gcggtctggg tgaccgggac gacgacagcg
     3421 tcggagccga gcgctttttt gacgttcgcc agctcgctgc cttcttcacc aaccgggccg
     3481 gagggcgcga tgtcggtgcc gatgaactcg cgcccttcga cgtggagaac gccgtcgtcg
     3541 gcgccgaagt tgtgcagggc ggcggcgctg ccccacggcc cgtaggcgct catctccggt
     3601 tttccccccg ccccctgcga gccgtcgcag gcaagcgggc tgctagaccc gttgaaaccg
     3661 aagggtttgt cgtggctcca acgagcctga gaggggtact ggaggtagcc cccgcccccc
     3721 tgaatgtcgc tgcccgagca ctgttcccca agcgccgagg cggcgggcgc ctgcagcgcg
     3781 gcgaaagcgg cgacgagcag ggccgcgtag atcaatcgac gaaacattgg ttcactcctt
     3841 tgatgtaagg gttgcctaat aatcagagga gattaatgcg ggcaatctgg ctctgggata
     3901 agcctggacg cccgttgaag atgtgaacga aatgcgaacg gaggggggtc tgggcggacc
     3961 tagactggcg ctgccgtggc gaccactccg atcctgctgc tctcgcttcc tcgcagcggc
     4021 tccaccgtgg tccagcgcgt gctcggggcg cacgaggagg tcgagacggc ctcggagtct
     4081 tggctcctgc tctcgctgct gctctcactg cgcgacgacg tgccggcgcc gggcgcctgg
     4141 cagccgcgga tccacgcggc gctcggggat ttcgccgccg agctgcccga cggggtcgct
     4201 tcatatgagc gggcggcggg gacggcggcg cgggcgctgt acgaggaagc ggccggcggg
     4261 gcgcgcttct atctggacaa gactcccccc tacttccttg tcgtcgacga gatcgtgcgg
     4321 gcgctgccgg aggcgcggat cgtcgtgctc tggcgcaacc cactggcggt gctcgcctcg
     4381 gtggtcgaga ccttctgtgg cgggcgctgg cgcccgcagg actacccgac cagcctcttc
     4441 ggcgggctcg aacggctggt cgccgcggtg ctgcgctacc cgagtcggat ccacacggtc
     4501 cgctacgagg acctggtcgg cggcgagcac gacgcctggc gggggctgac cgagttcgcc
     4561 ggtttcgagt tcgacccggg cagcc
//
LOCUS       DASRIG010000096         6240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_308083, whole genome shotgun sequence.
ACCESSION   DASRIG010000096 DASRIG010000000
VERSION     DASRIG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308083"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFB52_02650"
     CDS             complement(<1..547)
                     /locus_tag="VFB52_02650"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HZO05258.1"
                     /translation="MPAALGPDEIAELSAEIRELAAQRNAVLLAHNYMLPEVQDVADH
                     MGDSLGLSRQAAATVADTIVFCGVHFMAETASILSPEKTVLIPDPDAGCSLADSITAD
                     QLRAWKAQHPGALVVMYVNTSAEVKAETDYCCTSSNAVQVVEHIWAEHGPETEILFGP
                     DMFLGAYVEKVTGRRMRVWDGE"
     gene            663..1508
                     /locus_tag="VFB52_02655"
     CDS             663..1508
                     /locus_tag="VFB52_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZO05259.1"
                     /translation="MPELPEVEIVARRLDTALAGSTVESVLAPGMNVMKTFDPPLESL
                     AGRELTGVRRIGKMPVVEFGDLALLIHLMSAGRLRAFDKRASLKDRASRVLVRLEDGR
                     ELRLREFGTKQRAWAKLLPADAVAADEMVASLGPEAWPPPPLDEFAAAIDKPRHLHPL
                     LRHQKDISGIGRSWVDEILWEARLSPFKKGTELEAEEVERLHAALHVLGDAIDHYERM
                     IGPEVPDKVPMPLQIHKREGEPCPRCGTTIEAVFFSEHQMNYCPKEQTGGRVLKDRRL
                     SKLLK"
     gene            complement(1764..2621)
                     /gene="nadC"
                     /locus_tag="VFB52_02660"
     CDS             complement(1764..2621)
                     /gene="nadC"
                     /locus_tag="VFB52_02660"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZO05260.1"
                     /translation="MAEAADSLQEVVARALAEDLGAGDVTSEATVPAEARARARIVQK
                     APGVAFGFAAVAEAMAQCGVEHVDNLVVEGQWRDSVPADVIVASGSARSLLAAERTAL
                     NFLGHLSGIATLTARFVEAVQGTGAAILDTRKTTPGLRLLEKAAVAAGGGRNHRIGLY
                     DAFLIKENHIALAGGLARAVQGARANHPELPVEVEVRNLDEVAYALGTGAERLLLDNM
                     DPETMRAAVALRDQHSGAGEGPKLEASGGVTLETVRITAETGVDFISIGALTHSAPNL
                     DFSLLLEAA"
     gene            2678..3142
                     /locus_tag="VFB52_02665"
     CDS             2678..3142
                     /locus_tag="VFB52_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO05261.1"
                     /translation="MSELPPFQRLIDEHARDVMAVLRAAVGREGADDCFQETFLAALR
                     AYPGLRDDGNLRGWLITIAHRKAIDHHRARGRRPVPVAEVAEEAVPGPEPADDGVWAA
                     VGALPPKQRAAVTLRYASDLPHSEIAAALGCSPEAARRSLHEGIKRLRKELA"
     gene            3139..3714
                     /locus_tag="VFB52_02670"
     CDS             3139..3714
                     /locus_tag="VFB52_02670"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZO05262.1"
                     /translation="MSIERSLNRMRERAAAEGLLDVAYTTADSPYGPLLLATTPKGLV
                     RVGLPNQDYDELLEELARKVSPRVLEAAAPLDEARRELDRYFAGERERFELPLDWSLT
                     GGFQLKVQRQINRIPYGQTRTYTEMARRAGNERAVRAAGTACGRNPLPVVVPCHRVLR
                     SSGGLGGYGGGLPMKEGLLALEGILDERSPG"
     gene            3765..4454
                     /locus_tag="VFB52_02675"
     CDS             3765..4454
                     /locus_tag="VFB52_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05263.1"
                     /translation="MKRRPLPPGHQGTPFTIRLAWWAAFLATLALVFAIAQAKSAASA
                     ATLPAPVDALTGAIVSADDEESEEGEEEEWEYDEECETFGEGEEEEEFCEEIGGGEDG
                     TPPECLLSSAEATVSALPSRDTLRLQIRYTARSAAAVKVDFWLRGGKGPLNLGEDRKR
                     FGKTGTFRASERLTEAQMAKVLAARNFTVELQAVNTPRYCHRYFDQKLTVKRGLTWSE
                     PAARAAARTDG"
     gene            complement(4464..5105)
                     /locus_tag="VFB52_02680"
     CDS             complement(4464..5105)
                     /locus_tag="VFB52_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HZO05264.1"
                     /translation="MAPLVQLERSAAAEPAGILALHHGRGTDESDLLRLADYLDPEQR
                     LRVVSARAPLQLPGSPGYHWYLVPRVGYPDHDTFQAARAALAELHDGLWEETGVGPER
                     TVLGGFSMGAVMSYAMALSAERPAVAGILAFSGFVPTVDDWQPTFADRTKTRAFIAHG
                     RNDPIMEVGFAQRARDLLESGGLEVSYHESEIGHQIDPAHLAEATRWLGETLA"
     gene            complement(5108..6227)
                     /gene="prfB"
                     /locus_tag="VFB52_02685"
     CDS             complement(join(5108..6142,6144..6227))
                     /gene="prfB"
                     /locus_tag="VFB52_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157591830.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZO05265.1"
                     /translation="MADAAQTEAVAPRLAAVREQLSLLSDYLDPAGLKAEADRLEAEM
                     AAPGFWDDQETAAKTSAAHARAKKRLELFEGLEADVADLEELAEMAAEDEEMAAELDT
                     QLASAEQRLAELEEARLFSGKYDTGDAVVTVHAGAGGTDSQDWAEILLRMYLRWAERR
                     GFEVEMVEASPGEEAGLKSATFIARGENVYGLFAAERGVHRLVRISPFDSSSRRHTSF
                     AQVDVGPVVDDAVEVEIDDADLRIDTYRASGAGGQHVNKTDSAVRITHIPTGVVVQCQ
                     NERSQTQNKAVAMQLLRSKLIELEERKRTEELARARGEVKDVAWGSQIRSYVLHPYTM
                     VKDHRTEHEVGDAQRVLDGDIDEFIRDYLNKTAAASAS"
BASE COUNT          933 a         2202 c         2194 g          911 t
ORIGIN      
        1 actcgccgtc ccacacgcgc atcctgcggc ccgtgacctt ctcgacgtag gcgccgagga
       61 acatgtccgg gccgaagagg atctcggtct cgggaccgtg ctcggcccag atgtgctcga
      121 ccacctggac cgcgttcgag ctggtgcagc agtagtcggt ctccgccttc acctcggccg
      181 aggtgttcac gtacatgacc acgagggcgc ccgggtgctg cgccttccag gcacgcagct
      241 ggtcagcggt gatcgagtcg gcgagcgagc agccggcatc ggggtcgggg atcagcacgg
      301 tcttctcggg cgagaggatc gaggcggtct cggccatgaa gtggacgccg cagaagacga
      361 tcgtgtcggc cacggtggcg gccgcctggc gcgagagccc gagcgagtcg cccatgtggt
      421 cggcgacgtc ctgaacctcg ggcagcatgt agttgtgggc gaggaggacg gcgttgcgct
      481 gcgccgcaag ctcgcggatc tccgcgctga gttcagcgat ctcgtccggc ccaagggccg
      541 ctggcatcgt tggttggatc gtcgggagct cttgcatgag cccttccttt cctgatgttc
      601 gctggcgggc cgggatggcc gaaaccagtc gaatgaacgt ctcacaagtc tagcctgtgg
      661 gcatgcccga gctgcccgag gtcgagatcg tcgcccgccg cctcgacacg gcgctggccg
      721 gatcgacggt cgaatcggtg ctggcgccgg ggatgaacgt gatgaagacc ttcgacccgc
      781 cgctggaatc gctggccggg cgcgagctga ccggcgtgcg ccggatcggc aagatgcccg
      841 tcgtcgagtt cggcgacctg gcgctgctga tccacctgat gtcggccggg cgcctgcgcg
      901 ccttcgacaa acgcgcctcg ctcaaagacc gcgcctcgcg ggtgctggtc cggctcgagg
      961 acggccgcga gctgcgcctg cgcgagttcg gcaccaagca gcgcgcctgg gcgaagctgc
     1021 tgcccgccga cgcggtcgcc gccgacgaga tggtcgccag cctcggcccc gaagcctggc
     1081 cccctccccc gctcgacgag ttcgccgccg cgatcgacaa gccgcgccac ctgcacccgc
     1141 tgctgcgcca ccagaaagac atcagcggga tcggccgctc ctgggtcgac gagatcctct
     1201 gggaggcgcg cctctccccc ttcaagaaag gcacggaact ggaggcggag gaggttgagc
     1261 ggctacacgc ggccctgcac gtgctcggcg acgcgatcga ccactacgag cggatgatcg
     1321 gccccgaggt ccccgacaaa gtcccgatgc cgctccagat ccacaagcgc gagggcgagc
     1381 cctgcccccg ctgcgggacc acgatcgagg ccgtcttctt ctccgaacac cagatgaact
     1441 actgccccaa agagcagacc gggggccggg tgctcaaaga ccggcggctc tcgaaactgc
     1501 tcaagtagac gggcgtacct cgggacgaca tgcggggagg ggtcgggtga ctccctccac
     1561 ttaacttttt tgtggatcga ggggcggctt tacgcctcgg atcgctggag caaaaaagta
     1621 caaggcgttc cgggagtcac ccgacccctc cacacgggcc accccggggg ttccgtctgg
     1681 ctggcgggcc gtggcggggc tccgaacgca gttgttcccg cgtaccggga agaactgcgt
     1741 tcggagctgg ggcgttggtg ccgctacgcg gcttcgagca gaagagagaa gtcgaggttc
     1801 ggggctgagt gcgtcagggc tccgatggag atgaagtcga cgccggtttc agcggtgatg
     1861 cggacggtct cgagggtgac gcccccggag gcctccagct tcggcccctc ccccgcgccc
     1921 gagtgctgat cgcggagggc gacggcggcg cgcatcgtct ccgggtccat gttgtcgagc
     1981 agcaagcgct cggcgccggt gccgagcgcg taggcgacct cgtcgaggtt gcggacctcg
     2041 acctcgaccg gcagctcggg gtggttggcc cgggcgccct gcacggccct ggcgaggccg
     2101 cctgccaggg cgatgtggtt ctccttgatc aggaaggcgt cgtagaggcc gatccggtgg
     2161 ttgcgcccgc cgccggcggc gaccgcggcc ttctccagca ggcgcaggcc cggcgtcgtc
     2221 ttgcgggtgt cgaggatcgc cgcgccggtt ccctgcacgg cctcgacgaa acgagcggtc
     2281 agggtcgcga ttccggagag gtggccgagg aagttgagcg cggtgcgctc ggcggcaagc
     2341 agcgagcggg cgctgccgct ggcaacgatc acgtcggcgg gcacgctgtc gcgccactgg
     2401 ccctcaacca cgaggttgtc gacgtgctcg accccgcact gcgccatcgc ctcggcgacc
     2461 gcggcgaagc cgaaggcgac cccgggcgct ttctggacga tccgcgcccg cgcccgcgcc
     2521 tcggccggca ccgtcgcctc cgaggtcacg tcaccggcac cgaggtcctc cgccagcgcc
     2581 cgcgcgacca cctcctgcaa cgaatctgcg gcctcggcca tcgcggcacc ctacccgcat
     2641 ctcacgctcg cgccgctcgc cacgtttaat ccccagagtg agcgagttgc ccccgttcca
     2701 gcgactgatc gacgagcacg cgcgtgacgt gatggcggtg ctacgcgcag cggtggggcg
     2761 cgagggtgcg gacgactgct tccaggagac ctttctcgcc gccctgcgcg cctacccggg
     2821 gctgcgcgac gacggcaacc tgcgcggctg gctgatcacg atcgcccacc gcaaggcgat
     2881 cgaccaccac cgcgcccgcg gccgccggcc ggtcccggtc gccgaggtcg cggaggaggc
     2941 ggtcccgggg ccggagccgg ccgacgacgg cgtctgggcc gcggtcggag cgctgccacc
     3001 gaaacagcgg gcggcggtga ccctgcgcta cgccagcgac cttccccaca gcgagattgc
     3061 ggcggcgctc ggctgctcgc cggaggcggc gcgccgcagc ctccacgagg gcatcaaacg
     3121 actgcgaaag gagctggcat gagcatcgag aggagcctca accggatgcg cgagcgggcg
     3181 gccgcagagg gcctgctcga cgtcgcctac acgaccgccg actcgcccta cgggccgctg
     3241 ctgctggcaa cgacgccgaa gggcctggtc cgggtcgggc tgccgaacca ggactacgac
     3301 gagctgctcg aggagctggc gcgcaaggtc tcgccgcggg tgctggaggc ggcggccccg
     3361 ctcgacgagg cgcggcgcga gctcgaccgc tacttcgccg gtgagcgcga gcgcttcgag
     3421 ctgccgctcg attggagcct gaccggcggc ttccagctca aggtgcagcg ccagatcaac
     3481 cgcatcccct acggccagac ccgcacctac accgagatgg cgcgccgggc cggcaacgag
     3541 cgcgccgtgc gcgccgccgg taccgcctgc gggcgcaacc cgctgccggt ggtcgtcccc
     3601 tgccaccgcg tcctgcgcag cagcggcggc ctcggcggct acggcggcgg gctgccgatg
     3661 aaggaaggat tgctggccct ggagggcatc ctggacgaac gttcgcccgg ttaggctcgc
     3721 ccgggttaaa ggaatgctcg tttcatcccg attccttacg ggcaatgaaa cgccgtcccc
     3781 ttcctcccgg ccaccaggga accccgttca cgatccggct cgcctggtgg gccgccttcc
     3841 tcgccaccct ggctctggtc ttcgcgatcg cgcaggccaa gtcggccgca agcgccgcga
     3901 ccctgcccgc cccggtcgac gcgctgaccg gcgcgatcgt ctccgccgac gacgaagaat
     3961 ccgaagaagg cgaagaagaa gaatgggaat acgacgaaga atgcgaaacc ttcggtgaag
     4021 gcgaggaaga agaagaattc tgcgaagaaa tcggcggtgg cgaggacggg accccgcccg
     4081 agtgcctgct gagcagcgcc gaggccaccg tctccgccct cccctctcgc gacaccctgc
     4141 gcctgcagat ccgctacacc gcccgctccg ccgccgcggt caaggtcgac ttctggctgc
     4201 gcggcggcaa agggccgctg aacctcggcg aagaccgcaa gcgcttcggc aagaccggca
     4261 ccttccgcgc cagcgagcgc ctcaccgagg cgcagatggc caaggtcctg gcggcgcgca
     4321 acttcacggt cgagctgcag gcggtcaaca cgccgcgcta ctgccaccgc tacttcgacc
     4381 agaaactgac cgtcaagcgc ggcctcacct ggtcggagcc ggccgcccgc gccgccgctc
     4441 gcaccgacgg ctagcgccgc cgctcaggcc agcgtctccc ccagccagcg cgtcgcctcg
     4501 gccaggtgcg ccggatcgat ctggtgtccg atctcggact cgtggtagct gacctcgagg
     4561 ccgccggact cgagcaggtc gcgggcccgc tgggcgaagc cgacctccat gatcgggtcg
     4621 ttgcggccgt gggcgataaa ggcacgggtc ttggtgcggt cggcgaaggt cggctgccag
     4681 tcgtcgacgg tggggacgaa gccgctgaag gcgaggatgc cggcgaccgc cggccgctcg
     4741 gcgctgagcg ccatcgcgta gctcatcacc gcgcccatcg agaagccgcc gagcacggtc
     4801 cgctccgggc cgaccccggt ctcctcccag agcccgtcgt ggagctcggc gagcgccgcg
     4861 cgggcggcct ggaaggtgtc gtggtcggga tagccgaccc gcgggaccag gtaccagtgg
     4921 tagccgggcg agcccggcaa ctgcagcggc gcccgcgccg agaccacgcg caggcgctgc
     4981 tcggggtcga ggtagtcggc gagccgcaac aggtcgctct cgtcggtgcc gcggccgtgg
     5041 tgcagagcga gaatcccggc tggctcagcg gccgccgagc gttcgagctg gacgagcgga
     5101 gccacggcta gctcgccgag gcggcggcgg tcttgttcag gtagtcgcgg atgaactcgt
     5161 cgatgtcgcc gtcgagcacc cgctgcgcgt caccgacctc gtgctcggtg cggtggtcct
     5221 tgaccatcgt gtacgggtgc aggacgtagc tgcggatctg cgagccccag gcgacgtctt
     5281 tgacctcgcc ccgggccctg gccagctctt cggtccgttt gcgctcctcc agctcgatca
     5341 gcttcgagcg cagaagctgc atcgcgaccg ccttgttctg ggtctgcgag cgctcgttct
     5401 ggcactggac gacgaccccg gtcgggatgt gggtgatccg caccgcggag tcggtcttgt
     5461 tgacgtgctg gccgccggcg ccggaggcgc ggtaggtgtc gatgcgcaga tcggcatcgt
     5521 cgatctccac ctcgaccgca tcgtcgacga ccgggccgac gtcgacctgg gcgaagctcg
     5581 tgtggcggcg cgaggaggag tcgaagggcg agatccggac caggcggtgg acaccgcgct
     5641 cggcggcgaa gaggccgtag acgttctccc cgcgggcgat gaaggtggcc gacttcagcc
     5701 cggcctcctc ccccggcgag gcctcgacca tctcgacctc gaagccgcgc cgttcggccc
     5761 agcggaggta catccgcaga aggatctcgg cccagtcctg ggagtcggtc ccccccgccc
     5821 cggcgtggac cgtgaccacg gcgtcaccgg tgtcgtattt gccactgaac aggcgcgcct
     5881 cttcgagctc ggccaggcgc tgctcggccg aggccagctg cgtgtccagc tcggccgcca
     5941 tctcctcgtc ctccgcggcc atctcggcca gctcctcgag gtcggcaacg tccgcctcga
     6001 ggccctcgaa cagctccagc cgttttttcg cccgcgcgtg cgcggccgag gtcttggcag
     6061 cggtctcctg gtcgtcccag aagcccggcg ccgccatctc cgcttcgagg cggtcagcct
     6121 cggctttcag cccagccggg tcaaaggtag tccgagagca gggacagctg ctcgcggaca
     6181 gcggcgaggc gcggcgctac ggcttcggtc tgcgcggcat cggccatcgc tgaagactag
//
LOCUS       DASRIG010000097         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2663031, whole genome shotgun sequence.
ACCESSION   DASRIG010000097 DASRIG010000000
VERSION     DASRIG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663031"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFB52_02690"
     CDS             complement(<1..1178)
                     /locus_tag="VFB52_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO05266.1"
                     /translation="MTSRAACKGLVCLVVAALAVAGLSACGDSSEGSGPRKLTFFVAI
                     QPGGTIEENAKRCTEESNGKYEITPEFLPTDASQQREQLVRRLGAEDSSIDIVGMDVI
                     WTGEFANAGWVEEWTGKRKGEVTEKVFPGVIETASFEGKLYAAPFNTNTQLLWYRKDL
                     VKQAPRTWDEMIDQAEELGRDNAGTIQVQANRYEGFTVWANALIESAGTQLLSGPEEV
                     DLAEGPTTRALEVMGRFANSSAAPASLSTSNEDTARIGFESGESAFMTNYTFAYASAS
                     EEAPDIAKVMGFARFPRVDASRPSKPPLGGFNLAVSSFSENKDIAFDAAACLAGRESQ
                     KTAVELDGLPPSRSDLYATKTVTEAYPGFAELVKESIEDAGPRPSTAAYQDVSLAVQR
                     S"
     gene            complement(1271..2194)
                     /locus_tag="VFB52_02695"
     CDS             complement(1271..2194)
                     /locus_tag="VFB52_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05267.1"
                     /translation="MRVPGGITVLVAIALIAASPASAATEAGDGCTANVAEGPYSLVP
                     EAQASPSPLPLATPVGGVVTRWKVNSTYMEPVPERLGVFRPTGTAGRFLVVGESNEEL
                     VPVGGGSFATRIPVQAGDRFGPIAVINDTLYCLTGNAADKAWAFNGSAGTGSTHTFAA
                     GIEVRVPMVVTVEPDGDGDGYGDESQDGCPQSALTHLACPTVSLATFAVVKPNAVLLL
                     VSTSSTAPVAVTGSVKAGKGKPLALSAAKQTVSPGGLARFKLAFPKRLSGALAELESS
                     KSLKLKVKATATDVAGRAAVSASTVKLRGRG"
     gene            complement(2213..2830)
                     /locus_tag="VFB52_02700"
     CDS             complement(2213..2830)
                     /locus_tag="VFB52_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05268.1"
                     /translation="MGITRIADAEAIEPKRRDGRERLLNGARKLLAEKGYAGMELRDV
                     AEVGDAPRGSIYHHFPGGKIQLAVEATAREGAEVQTAIERSLEERGLAATLAMFGEMF
                     ARRVKDHPERLGCPVAAAALARPEDPALAAAATAAFASWEGPIAAALRDEGVGEQDAA
                     AFAGLVVSTIEGALIRARAAADRAPLDSAVIGLERALDSLLASTS"
     gene            2882..>3313
                     /locus_tag="VFB52_02705"
     CDS             2882..>3313
                     /locus_tag="VFB52_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05269.1"
                     /translation="MAVACAGAFVAFLDTTIVNIAFPDIAASFPEASRGLLSWVLDGY
                     FVVIAALLVPAGGLADRFGHRRVFMLGVVAFTAASLLCAVAPSLELLIGFRVLQGVGA
                     ALIAPASLAIVLDSFPVERRSTGVGLWGAAAAAAAAVGPTLG"
BASE COUNT          423 a         1146 c         1189 g          555 t
ORIGIN      
        1 gagcgctgca cggcgaggct gacgtcctgg taggcggcgg tgctcggccg cggcccggcg
       61 tcttcgatcg attccttgac cagctcggcg aagcccgggt aggcctcggt cacggttttg
      121 gtcgcgtaga gatcggagcg ggagggcggc aggccgtcga gttcgaccgc ggttttctgg
      181 ctctcccggc cggcgagaca ggcggcagcg tcgaaggcga tgtccttgtt ctccgagaag
      241 gagctgaccg ccaggttgaa gccgcccagc ggtggcttgc tcgggcggct ggcgtcgacc
      301 cgcgggaaac gggcgaagcc catgaccttg gcgatgtccg gtgcttcttc ggaggcgctg
      361 gcgtaggcga aggtgtagtt ggtcatgaag gccgattcac ccgactcgaa gccgatgcgg
      421 gcggtgtcct cgttggaggt cgagaggctc gcgggcgccg ccgaggagtt ggcgaagcga
      481 cccatcacct ccagcgccct cgtcgtcggg ccctcggcca ggtcgacttc ctctgggccg
      541 gagaggagct gcgtgccggc gctctcgatc agggcgttgg cccagacggt gaagccctcg
      601 tagcggttgg cctgcacctg gatcgtgccg gcgttgtcac ggccgagctc ttccgcctgg
      661 tcgatcatct cgtcccaggt gcgcggcgcc tgcttgacga ggtccttgcg gtaccagagc
      721 agctgggtgt tggtgttgaa cggcgccgcg tagagcttgc cctcgaagga ggcggtttcg
      781 atcacgccgg ggaagacttt ttcggtcact tcccccttgc gcttgccggt ccactcttcg
      841 acccagccgg cgttggcgaa ctcgccggtc cagatcacgt ccatgccgac gatgtcgatc
      901 gaggagtcct cggcgccgag ccggcggacc agctgttcgc gctgctggct cgcgtcggtg
      961 gggaggaatt ccggcgtgat ctcgtacttg ccgttcgact cttcggtgca gcgcttggcg
     1021 ttttcctcga tcgtgccgcc gggctggatc gcgacgaaga aggtgagctt gcgcgggccc
     1081 gagccttcgg agctgtcgcc gcaggcgctc agcccggcaa ctgccaaggc ggcgaccacc
     1141 aggcagacga ggcccttgca cgcagcgcgg gatgtcatga ctcctcctcc atgacgccgc
     1201 ccccggaccc gcgagcaaag tttgcggagg cgcccttaag tgatgcccgg cgctcgggct
     1261 tctaaacgcg ctagccgcgg ccgcgcagct tcacggtgct ggccgagacc gcggcgcggc
     1321 cggcgacatc ggtggccgtc gcctttactt tcagcttcag cgacttgctg ctctcgagct
     1381 ccgcgagagc gccgctcagc cgtttcggga aggcgagctt gaagcgggcg aggccgccgg
     1441 gggagacggt ctgtttcgcg gcggagaggg ccagcggctt gcctttgccg gccttgaccg
     1501 agccggtaac cgcgaccggc gccgtgctcg aggtcgagac cagcagcagc accgcgttcg
     1561 gtttgaccac ggcaaaggtg gccagggaca ccgtcgggca ggcaaggtgg gtgagcgcgc
     1621 tctgcgggca gccgtcctgg ctctcgtcgc cgtagccatc gccgtcgccg tcgggctcga
     1681 ccgtgacgac catcgggacc cgaacttcga tgcctgccgc gaaggtgtgc gtcgagccgg
     1741 tcccggcgct gccgttgaag gcccaggctt tgtcggcggc gttgccggtc aggcagtaga
     1801 gcgtgtcgtt gatcacggcg atcgggccga agcggtcccc ggcctggacc gggatgcggg
     1861 tggcgaagga gccgccgccg acgggcacca gctcctcgtt gctctcgccg acgaccagga
     1921 acctgccggc cgtccccgtc ggccggaaca cccccagccg ctccggcacc ggttccatat
     1981 acgtggagtt gaccttccat ctggtgacga cgccgccaac cggagtcgca agcggcagcg
     2041 ggctcgggct cgcctgcgcc tccggaacca ggctgtatgg gccttcggcg acgttcgccg
     2101 tgcagccgtc gccggcttcg gtcgccgccg acgccggcga cgcggcgatc aaagcgatgg
     2161 ccaccagcac ggtgatcccc ccgggtacgc gcatggaatg accctatagc ggtcagctcg
     2221 tgctggccag caaactgtcc aacgcccgct cgagcccgat gacggcgctg tcgagcggcg
     2281 cccggtcggc ggcggcgcgg gcgcggatca gggcgccctc gatcgtcgag acgacgaggc
     2341 ccgcgaaggc cgcggcgtcc tgctcgccga caccttcgtc gcgcagggcg gcggcgatcg
     2401 gcccttccca gctcgcgaag gcggcggtcg cggcggcggc cagggcgggg tcctcgggcc
     2461 gggcgagggc ggcggcggcg accgggcagc cgaggcgctc cgggtggtcc ttgacccggc
     2521 gcgcgaacat ctcgccgaac atggccagcg tcgcggccag gccgcgctcc tccagggagc
     2581 gctcgatcgc ggtctggacc tcggcgccct cgcgggcggt cgcctcgacc gcgagctgga
     2641 tcttgccgcc cgggaagtgg tggtagatcg agccgcgcgg ggcgtccccg acctcggcca
     2701 cgtcgcgcag ctccatcccg gcatagccct tctcggcgag cagcttccgg gctccgttca
     2761 gcagtcgctc acgaccgtcg cggcgcttcg gttcgatcgc ttccgcgtct gcaattcgcg
     2821 ttatgcccac ggacatagta tagtcgccgc ccagatgtat gaccagcgtc atagaggggc
     2881 cgtggcggtc gcatgtgccg gcgcttttgt cgccttcctc gacacgacga tcgtcaacat
     2941 cgccttcccc gacatcgccg cctccttccc cgaggcgagc cgcggactgc tctcctgggt
     3001 gctcgacggc tacttcgtgg tgatcgcggc gctgctcgtc ccggcgggcg ggctcgccga
     3061 tcgcttcggc caccgccgcg tcttcatgct cggcgtcgtc gccttcaccg ccgccagcct
     3121 gctctgcgcg gtcgcaccct cactcgagct gctgatcggc ttccgcgtcc tgcagggcgt
     3181 gggggcggcg ctgatcgcgc cggcctcgct ggcgatcgtg ctcgacagct tcccggtcga
     3241 gcgccgctcg accggggttg gcctctgggg cgcggcggcc gccgcggccg ccgcggtcgg
     3301 accgacgctc ggc
//
LOCUS       DASRIG010000098         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1351662, whole genome shotgun sequence.
ACCESSION   DASRIG010000098 DASRIG010000000
VERSION     DASRIG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351662"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFB52_02710"
     CDS             complement(<1..140)
                     /locus_tag="VFB52_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05270.1"
                     /translation="MSGRLADRVAVVTGGGRGIGRAIALGFAGEGATVALLARSADQL
                     EAV"
     gene            complement(137..925)
                     /locus_tag="VFB52_02715"
     CDS             complement(137..925)
                     /locus_tag="VFB52_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO05271.1"
                     /translation="MWKVEERDSGVVVARFDRPPMNYYTDEDIGQLEGLVAEWTARQV
                     PAVVLAGGRPGKFITHFEVDGILLKQVEPEGPIESPARSRRIHLLTEAMNEAPMPILA
                     ALNGDAMGVGFEFALSSDIRVGERADHRYGLIETRLGIVPGGTGLTRMARMIGPGKAM
                     LLGAGARCLTPEEAYEHGLVELLEDDAFATALEVAERMAELPRTAVAMAKRIVYQSQG
                     VPLSVALDFELDSAYRAKQSPEAAPALRDYLSRPHEERRAWFER"
     gene            complement(1006..2145)
                     /locus_tag="VFB52_02720"
     CDS             complement(1006..2145)
                     /locus_tag="VFB52_02720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZO05272.1"
                     /translation="MRTPRTPALLIAALACAAALAPAASAKSTPADVQRGLERLIAAP
                     GGPPGAIATLYRNGKLTVLRAGRADVKRKGAPRATDHMRIASVAKAFSGAVALNLVRA
                     GKLGLDDTIAQRLPGAMPTVWGAVTVRQMLGHTSGLPDYTKSKPFAEQAEKNPRGFVT
                     PQTVIDWVRGDGLEFAPGSKYEYSNTDNIVIGLIVEAITGEPYGAALQKVVFGPARLR
                     QTTFPTRKVALPAPFIHGYIAQPGEEPADVTTFLSPSGAWASGAIVSTPRDLNAFIRA
                     DLGLRFFDRAQQQEQMRFWPGGESSPPGPGRNSAGLALFRYQTKCGTVYGHTGNFPGY
                     VQFAAATADGSKAVTTSLNIPAPKGKLLAQLRGVQATAVCALLGK"
     gene            complement(2194..2394)
                     /locus_tag="VFB52_02725"
     CDS             complement(2194..2394)
                     /locus_tag="VFB52_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05273.1"
                     /translation="MLAVHSPSAQSYSYPPAEVTDRIFRPQVQGGFGVQLPQYVGEAY
                     EDYGDEGSPPGPSPPTYIAYCH"
     gene            complement(2553..>3425)
                     /locus_tag="VFB52_02730"
     CDS             complement(2553..>3425)
                     /locus_tag="VFB52_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05274.1"
                     /translation="LIEFVTGKPIPYETIWITGNSKDGQRQRGMFPPSVRQRKVELVW
                     HRGTLEQWVGIARGVPHPHGQHWVELPVLGTTAQVDTRAEFYVNAGGPGDRQMTAFWA
                     EDGQVLELRAAVPDLAAFEERLGWLTKVDSQTWLDAMPAKVVKAADHDSAVREMLEGI
                     PVPSTFSPSRVPDEGLTTNRYQVGAAVTGTVSCLWFRQWGTARRTGDRAAEAEAERAM
                     ATSKRWPILREMAQDGAYPATIWRLAKSMPSDEWRRGQRLLPHAEGLGCARWGIPVLP
                     WKQRRQNERERTGG"
BASE COUNT          451 a         1152 c         1289 g          533 t
ORIGIN      
        1 accgcttcga gctggtcggc gctgcgcgcc agtagcgcga cggttgcgcc ctcgccggcg
       61 aagccgagcg cgatcgccct gccgatcccg cggccgccgc cggtcacaac cgcgacccgg
      121 tctgccagtc gcccactcac cgttcgaacc aggcccgccg ctcctcgtgc ggccgggaga
      181 ggtagtcgcg caaggcgggc gccgcttccg gggactgctt tgcccggtag gcgctgtcga
      241 gctcgaagtc gagtgcgacc gagagcggca cgccctggct ctggtagacg atccgcttcg
      301 ccatcgcgac cgcggtgcgc ggcagctccg ccatccgctc ggcgacctcg agcgcggttg
      361 cgaaggcgtc gtcctcgagc agctcgacca gcccgtgctc gtaggcctcc tcgggggtca
      421 ggcagcgggc gccggcgccg agcagcatcg cctttccggg gccgatcatc cgcgccatcc
      481 gggtcagccc ggtgccgccg gggacgatcc ccaggcgcgt ctcgatcagc ccgtagcggt
      541 ggtcggcgcg ctcgccgacg cggatgtcgg aggagagggc gaactcgaac ccgaccccca
      601 tcgcgtcgcc gttgagggcg gcgaggatcg gcatcggcgc ctcgttcatc gcctcggtca
      661 gcaggtggat gcggcggctg cgcgccggcg actcgatcgg tccctccggc tcgacctgct
      721 tgagcaggat cccgtcgact tcgaagtggg tgatgaactt gccggggcgg ccgccggcga
      781 gcacgaccgc gggcacctgg cgcgcggtcc attccgcgac cagcccctcg agctggccga
      841 tgtcctcgtc ggtgtagtag ttcatcggcg gccggtcgaa ccgcgcgacg actacaccgc
      901 tatctcgctc ctcgaccttc cacatcgtcc cgagcgtacg gcggcgccca cgccagcgcg
      961 agcgccgatc cgcagccgct cgggactatc gacaaaaggg gatcgtcact tccccagcag
     1021 cgcgcagacc gcggttgcct ggacgccgcg cagctgggcg agcagcttgc ccttgggggc
     1081 ggggatgttg agcgaggtgg tgaccgcctt ggacccgtcc gcggtcgccg cggcgaactg
     1141 gacgtagccg gggaagttgc cggtgtgacc gtagaccgtg ccgcatttcg tctggtagcg
     1201 gaagagggcg agaccggccg agttgcggcc ggggcccgga gggctcgatt cgccgccggg
     1261 ccaaaagcgc atctgctcct gctgctgggc gcggtcgaag aagcgcaggc cgaggtcggc
     1321 gcggatgaag gcgttgaggt cgcggggcgt ggagacgatc gcgcccgagg cccaggcgcc
     1381 gctggggctg aggaaggtgg tgacgtcggc cggctcctcg ccgggctgag cgatgtagcc
     1441 gtggatgaac ggcgcgggca gggcgacctt tcgggtcggg aaggtggtct ggcgcagccg
     1501 ggccgggccg aagacgacct tctgcagggc cgcgccgtag ggctcaccgg tgatcgcctc
     1561 gacgatcagg ccgatcacga tgttgtcggt gttcgagtac tcgtacttcg accccggcgc
     1621 gaactcgagc ccgtccccgc gcacccagtc gatcacggtc tggggcgtga cgaagccgcg
     1681 cgggttcttt tccgcctgtt cggcgaacgg tttcgacttc gtgtagtcgg gcaggccgct
     1741 ggtgtggccg agcatctggc gcacggttac cgcgccccag acggtgggca tcgcgcccgg
     1801 aagccgctgg gcgatcgtgt cgtcgaggcc gagcttgccg gcgcggacga ggttgagcgc
     1861 gaccgcgccg ctgaacgcct tggcgacgct ggcgatccgc atgtggtcgg tcgcccgcgg
     1921 ggcgcccttg cgcttgacgt cggcgcgccc ggcgcgcagc acggtcagct tgccgttgcg
     1981 gtagagcgtc gcgatcgccc cgggagggcc gcccggcgcg gcgatcagtc gctccagccc
     2041 gcgctggacg tcggcggggg tcgacttcgc ggaggcggca ggcgccagcg cggcggcgca
     2101 ggcgagggcg gcgatcagca gggcgggggt gcggggagtg cgcatcgggt ccttccgggg
     2161 agggtggggg tgttcagggt aagggcccgc cgctcagtgg cagtaggcga tgtaggtggg
     2221 cggcgacggg ccggggggcg aaccttcgtc accatagtct tcgtaggcct ccccgacgta
     2281 ttgcgggagc tgcacgccga agccgccctg gacctgcggc cggaagatcc ggtcggtgac
     2341 ttcggccggc gggtaggagt aactctgcgc cgacgggctg tggaccgcga gcacgaacgg
     2401 gctcgggaag ttgccgtgga tcgtctgtat gggtctgctc gcgcgaggac ctgaaggcgc
     2461 tgaagcgagc ggcgggccga acccgcgacc tcgccgatct cgaggatctg gacgctacgg
     2521 ccgactagct gcgcggagga gcaccggaca gctcagccgc ccgtgcgctc acgttcgttc
     2581 tggcgccgct gcttccacgg caggaccggg atgccccagc gggcgcaccc gaggccttcc
     2641 gcgtggggca gcagccgttg gccgcgccgc cattcgtcgc tgggcatcga tttggcgagg
     2701 cgccagatcg tggcggggta ggcgccgtcc tgcgccatct cccgcaggat cggccagcgc
     2761 ttcgaggtcg ccatcgcccg ttcggcttcg gcctcggcgg cgcggtcgcc ggtccttcgg
     2821 gccgtccccc actgccggaa ccagagacag gagacggtgc cggtgaccgc ggcgccgacc
     2881 tggtagcggt tcgtcgtcag cccctcgtcg ggaacccggc tggggctgaa ggtggaaggc
     2941 acggggatgc cctcgagcat ttcgcggacc gcgctgtcgt gatcggccgc tttcacgact
     3001 ttcgccggca tcgcgtcgag ccaggtctgg gagtccacct tggtgagcca gccgagccgt
     3061 tcttcgaagg cggccaggtc ggggaccgcg gccctcagtt cgagcacctg gccgtcttcg
     3121 gcccagaacg cggtcatctg acggtcgccg ggaccgcctg cgttgacgta gaactcggcc
     3181 cgggtgtcga cctgcgcggt cgtccccaga accggcagct cgacccagtg ctggccgtgc
     3241 gggtggggga ctccccgggc gattccgacc cactgctcga gcgtgcctcg gtgccagacg
     3301 agttcgactt tgcgctggcg cacagagggc gggaacattc cccgctggcg ctgtccgtct
     3361 ttgctgttgc cggtgatcca gatcgtttcg taggggatcg gcttgccggt gacaaattcg
     3421 atcag
//
LOCUS       DASRIG010000099         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3704772, whole genome shotgun sequence.
ACCESSION   DASRIG010000099 DASRIG010000000
VERSION     DASRIG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3704772"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1569
                     /locus_tag="VFB52_02735"
     CDS             178..1569
                     /locus_tag="VFB52_02735"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZO05275.1"
                     /translation="MSGTEQEARPTEVSMEEIVALCKRRGFVFPSSEIYGGVGSTYDY
                     GHYGVLLKTNVKNEWWRAMLQDRDDIVAIDSAIIQSPKVWEASGHLAGFSDPLVQCLG
                     KCKKRWREDHLREAVEAEGGDPDAELRCPECGGELSEPRQFNLMFETHVGPVAGEGSE
                     VYLRPETAQGIFINFKNVLGFARKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEM
                     EFFVPPADAERWYREWIEIRERWYVDLGIRPDFLRVRPHDADELSHYSSGTSDVEYLF
                     PIGWSELEGIANRGDFDLKAHATHSGEKLEYFDQATKERYVPHVIEPAAGADRATLAF
                     LVDAYEVEEVEGRQRTVLRLHPRLAPVKVAVLPLVSKEGMPEKAREIFQALRRRVPAE
                     FDEGGSIGKRYRRQDEIGTPWGITVDHQTMEDDTVTLRDRDSLEQTRVPVEGLTELLA
                     GKLEAPWQSPKLG"
     gene            1620..3494
                     /locus_tag="VFB52_02740"
     CDS             1620..3494
                     /locus_tag="VFB52_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HZO05276.1"
                     /translation="MTKSKLPLAAGLVLVALFAMTAGANAKSSVGGLPDLVVTKVSKP
                     GAQKALGSKLKFVATVKNKGGGAAGKSKLGLYLGKGKKHVKKDKRLKLVKVKPLAAGK
                     QQKRKFQVVLPKKAATGQYRLFACADATKKVNEGKEANCKATRKFKLVPATTPKPAAP
                     AFTMTDGFDWGFATNAKQESVNAGSPITLTLRTANGLAGQAGYTQSAVAPAPFLTGTT
                     IPLNFSEQDDGIAEIALPFAFPFGGVNETSLSVGTNGWVSFGAPAWDYWDDSQPIYYR
                     GPLVAVGEFYRGLMPYWSDLDLETAGGGTVKEVISADGKSVAIQWDIGSHQGDEAPKR
                     AFQVVLSADGSFRFDYPGANLPGGYKSFVGFSTGSGPAAAGVVSIEGTSVPTTSVQFT
                     PNPAQAETATAAGQISATLPFGSSLVSADPGCVLAKPPTLIDNGLVNCSVPGIFPGEQ
                     LTRHVTFAAPANAAVASEPPNFSFQGSYEAGPFTLKDGKEINSLTTSLPPLTFVLDAK
                     YPGAAPAVGDPALFEVQYGGPTSGGFDEPTVTFSLPANAAVDGISIDGKALPCGPGSG
                     TQVTCKLPSGSNGATIKVTVIPGSAAQGNPLTLGASFSALNAPTATDSVSTPPVTAAA
                     "
     gene            3554..>4012
                     /locus_tag="VFB52_02745"
     CDS             3554..>4012
                     /locus_tag="VFB52_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05277.1"
                     /translation="MRRIALIALAVALAATVVSVAVAAPAQQRAAKAPRVGIGPATAL
                     GPAKAVSKAGKNRPGDSKTTKPANAGGSTANANVLFSDSFDRADGLITNEYATWNPNS
                     SQAVKSADWQMTSGSLFAKGGAGWTGVPDGCSSASASGSPCTASAVFRLNT"
BASE COUNT          701 a         1469 c         1289 g          553 t
ORIGIN      
        1 aagtggaccg cgggcgagtg cagcacgcgc accgcggggg cgccgcactc ctcgcatttg
       61 gtcagcggat catccgccat ccgctgcatc acgtcgaaga cgtggccgtt gtcgcatttg
      121 tactcgtaga taggcacgaa gtgcgggatt atcgcagccg ctacggttca gccacttatg
      181 agtggcaccg agcaggaggc aaggcccacc gaggtctcga tggaggagat cgtcgcgctg
      241 tgcaaacggc gcggcttcgt ctttccctct tcggagatct acggcggcgt cggctcgacc
      301 tacgactacg gtcactacgg cgtcctgctc aagaccaacg tcaagaacga gtggtggcgg
      361 gcgatgctgc aggatcgcga cgacatcgtc gcgatcgact cggcgatcat ccagagcccg
      421 aaggtctggg aggcgtccgg gcacctggcc ggcttctccg acccgctggt ccagtgcctc
      481 ggcaagtgca agaaacgctg gcgcgaggat cacctgcgcg aggcggtcga agccgaaggc
      541 ggcgaccccg acgcggaact gcgctgcccc gagtgcggcg gcgagctatc cgagccacgc
      601 cagttcaacc tgatgttcga gacccacgtc ggcccggtcg ccggcgaggg ctccgaggtc
      661 tacctacgcc ccgagaccgc ccagggcatc ttcatcaact tcaagaacgt gctcggcttc
      721 gcccgcaaga agccgccgtt cgggatcgcc caggtcggca agtccttccg caacgagatc
      781 accccgggca acttcatctt ccgcacccgc gagttcgagc agatggagat ggagttcttc
      841 gtgccgccgg ccgacgccga gcgctggtac cgcgagtgga tcgagatccg cgagcgctgg
      901 tacgtcgacc tcggcatccg ccccgacttc ctgcgcgtgc gtccccacga cgccgacgag
      961 ctctcccact actcctccgg gaccagcgac gtcgagtacc tcttcccgat cggctggtcg
     1021 gagctcgagg gcatcgccaa ccgcggcgac ttcgacctca aggcccacgc cacccacagc
     1081 ggcgagaagc tcgagtactt cgaccaggcg accaaagagc gctacgtccc gcacgtgatc
     1141 gagcccgccg ccggcgccga ccgcgccacc ctcgccttcc tcgtcgacgc ctacgaggtc
     1201 gaggaggtcg agggccgcca gcgcaccgtg ctgcgcctgc acccgcgcct cgccccggtc
     1261 aaggtcgcgg tgctcccgct cgtctccaag gagggcatgc cggagaaggc ccgcgagatc
     1321 ttccaggccc tgcgccgccg cgtccccgcc gagttcgacg agggcggctc gatcggcaag
     1381 cgctaccgcc gccaggacga gatcggcacc ccctggggca tcaccgtcga ccaccagacg
     1441 atggaggacg acaccgtcac cctgcgcgac cgcgactcgc tcgagcagac ccgcgtcccg
     1501 gtcgagggcc tcaccgagct gctggccggc aagctggaag cgccctggca gagcccgaag
     1561 ctgggctagg cgtccagccg cgaactggcg tgttcgccgc cctgtagggt gccgcgccga
     1621 tgactaagag caagcttccc ctcgctgccg ggctcgtgct ggtcgcgctt ttcgcgatga
     1681 ccgccggagc aaatgccaag tcaagcgtgg gagggctgcc cgacctggtc gtgaccaagg
     1741 tctcgaagcc aggcgcgcag aaggcgctcg ggagcaagct gaagttcgtc gccacggtga
     1801 agaacaaagg cggcggcgcg gccggcaaga gcaagctcgg cctctacctc ggcaagggca
     1861 agaagcacgt caagaaggac aagcggctga agctggtcaa ggtcaagccg ctggccgccg
     1921 gcaagcagca gaagcggaag ttccaggtcg tgctgccgaa gaaagcggca accggtcagt
     1981 accgcctctt cgcctgcgcc gacgcgacca agaaggtgaa cgagggcaag gaggccaact
     2041 gcaaggccac ccgcaagttc aaactggttc cggcgacgac gccgaaaccg gcggcccccg
     2101 ccttcacgat gaccgacggc ttcgactggg gcttcgctac caacgcgaaa caagaatcgg
     2161 tcaatgccgg ctcgccgatc acgctgaccc tgcggaccgc caacggcctc gccggccagg
     2221 ccggctacac ccagtccgcg gtcgcgcccg ctccgttcct gaccgggacg acgatcccac
     2281 tcaacttcag cgagcaagac gacggcatcg ccgagatcgc gctccccttc gcgttcccgt
     2341 tcggcggcgt gaacgagacc tcgctgagcg tcggcaccaa cggctgggtc agcttcggcg
     2401 ccccggcctg ggactactgg gacgacagcc agccgatcta ctaccgcggt ccgctggtcg
     2461 ccgtcggcga gttctaccgc ggcctgatgc cctactggtc ggacctcgac ctcgagaccg
     2521 ccggcggcgg cacggtcaaa gaggtgatct ccgccgacgg caaatcggtc gcgatccagt
     2581 gggacatcgg ctcgcaccag ggcgacgaag ccccgaagcg cgccttccag gtcgtgctct
     2641 ccgccgacgg cagcttccgc ttcgactacc cgggcgcgaa cctgcccggc gggtacaaat
     2701 cgtttgtcgg cttctcgacc ggcagcggac cggctgccgc cggagtcgtc tcgatcgaag
     2761 gcacttcggt cccaaccacg agcgtccagt tcaccccgaa cccggcgcag gccgagaccg
     2821 cgaccgcggc cggccagatc agcgccacgc tcccgttcgg gtcatcgctg gtctccgccg
     2881 accccggctg cgtgctggcg aaaccgccga ccctgatcga caacgggctg gtcaactgct
     2941 cggtccccgg catcttcccg ggcgaacagc tgacccgcca cgtgacgttc gcggcaccag
     3001 ccaacgcggc ggtcgcctcc gagcccccga acttcagctt ccagggctcc tacgaggccg
     3061 ggccgttcac gctcaaagac ggcaaggaga tcaactcgct caccaccagc ctgccgccgc
     3121 tgaccttcgt gctcgacgcg aaatacccgg gcgccgcacc ggccgtcggg gacccggcgc
     3181 tgttcgaagt ccagtacggg gggcccacca gtggcggctt cgacgaaccg acggtgactt
     3241 tctcgctgcc cgccaacgcc gcggtcgacg ggatctcgat cgacggcaaa gcgctcccat
     3301 gcggcccggg ctcaggcacc caggtgacct gcaaactccc gtcggggtca aacggggcga
     3361 cgatcaaagt gaccgtgatc ccgggctcgg ccgcgcaagg caacccgctg acgctcggcg
     3421 cctcattcag cgccctcaac gcgccgaccg ccaccgacag cgtcagcacg ccgcccgtga
     3481 ccgcggcggc gtagccgtcc agaaccgtcc agaccgctaa acagacgcgc tggggggccg
     3541 atatggcgtt tagatgcgcc gtatcgccct catcgcgctg gcggtcgccc tggcggcgac
     3601 cgttgtcagc gtcgccgtcg ccgcgccggc gcagcagcgc gccgccaagg cgccgcgggt
     3661 ggggatcggg ccggcgaccg cgctcgggcc cgccaaggcg gtctcgaagg cgggcaagaa
     3721 ccggcccggc gactcgaaga ccaccaagcc ggccaacgcg ggcggctcaa ccgccaacgc
     3781 gaacgtgctc ttctccgact ccttcgaccg cgccgacggg ctaatcacca acgagtacgc
     3841 gacctggaac ccgaacagct cccaggcggt gaagtcggcc gactggcaga tgacctcggg
     3901 ctcgctgttc gccaagggcg gcgccggctg gaccggggtc ccggacggct gctcctcggc
     3961 cagcgccagc ggctccccct gcaccgcctc cgcggtcttc cggctcaaca cc
//
LOCUS       DASRIG010000100         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3705784, whole genome shotgun sequence.
ACCESSION   DASRIG010000100 DASRIG010000000
VERSION     DASRIG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3705784"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFB52_02750"
     CDS             complement(<1..195)
                     /locus_tag="VFB52_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZO05278.1"
                     /translation="MSTETRDDLRNIAIIAHVDHGKTTLVDAMLWQTGTFRKGQDVAE
                     RVMDSMDLEREKGITILAKNT"
     gene            complement(305..508)
                     /locus_tag="VFB52_02755"
     CDS             complement(305..508)
                     /locus_tag="VFB52_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZO05279.1"
                     /translation="MPTGTVKWFSDDKGYGFITPDDGSKDVFVHHSAILGEGFKSLTE
                     GAKVTYESEEGPKGPAAASVQQN"
     gene            662..2926
                     /locus_tag="VFB52_02760"
     CDS             662..2926
                     /locus_tag="VFB52_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZO05280.1"
                     /translation="MRPVRKRLAARTATFVALAFSALVPVAAANAALVPLQHGPGTPL
                     RDGKRLIFDVRFERGAVAGLDELRAAGAEIVHTSRRYQTVTVAVKPADLPALDGVARV
                     GGVKEVVRPLVMGADCGGILRSEGDSQLLAPNARANIGVDGTGVTVGILSDSYDRDPT
                     APTRAVTDVATGDLPGPGSPCGSTAPVNVLDDSVQESDEGRAMAQIVRDLAPGASISF
                     ATAFKGEAAFAASIKALAQAGARVITDDVVYFEEPFFQDGPIAVAIDEVAAAGVSYFS
                     AAGNNNLFSGGNEVGSWEAPFRNAETCPPGLPSGEEQCADFNPGGGTDTLFNFSVPSE
                     GELILDLQWAEPRGGVATDFDAYLINSKGQIVDSSNDDNIGGTQRPYELVRTENTGES
                     AVPVWLAITRKAGSGTPRLKWVQLGNAVPAATQYETSSGDVFGPTVFGHAAAKGAIAV
                     GAVRFNTASAPETFSSRGPVTNYFGPVVGAGPAAPLALPEVVSKPDIAASDGGVNTFF
                     GSFTGGFWRFFGTSAAAPHAAAVAALALQANPGATPTQIRNALTSTARPVGTFGPLAV
                     GAGLIDAGAAVNSLALAPGVAVTVPPAARSRVRQPTFEFEASRPATYSCAIDGVAMPC
                     ASPFTWPQPLGDGPHSLVVGATDLAGRVGSSAAVGFTIDSKAPQTTIVKKPPKVVRTS
                     NFGARVVFRFRSSERGARFRCKIDKTGYRKCGKRLSKRFTLGAHVLRVKAADAVGNLD
                     PSPAVYRFRVVRKP"
     gene            complement(2923..3657)
                     /locus_tag="VFB52_02765"
     CDS             complement(2923..3657)
                     /locus_tag="VFB52_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZO05281.1"
                     /translation="MATGEQQHETSQAGESRERPRLPLTEIAITLGGIALLAALFLAI
                     PDLRDSVRYAIEGDHEAVRKEIDDLGVWGPLLIFGLALIHTVVFYPAEIVDFAAGFAY
                     GFFPALVLTMFGWVVSGILCWLIGGSVARPLLDRWLGAERFERIEARIERGGVTLLLA
                     MRLIPILPFSIVSYAAGAARVPLWRFIWTTAVGYLPITALSVYFGTRLEGLSLTDPLV
                     FISAGVLLALLGAGHLVMRRQSAQQH"
     gene            complement(3702..>5391)
                     /locus_tag="VFB52_02770"
     CDS             complement(3702..>5391)
                     /locus_tag="VFB52_02770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZO05282.1"
                     /translation="QHPRGLNNFVRTVSDPASPRYRDYATVAQLVNRFGAKPQTKKQV
                     VRWFAARGLHAKVIGSGTHAIVSMTGAQASRLLPPADGAAASVTGLDRNIPAGLEGAV
                     TGISVVSAAPVVQPLAGPAVPTEAAASASKEITAADFGKPYRSILLHSGTAGGCPAGS
                     SGGEPGLPPFTPNQYLTAYGHSDLHAKGFKGQGQTVAVVETGGFKHSDIAAFAKCFGI
                     AKPPPIKVVPVEVPKPLPGEDETTLDLSMLAVGAPKLDKIYVYEGLESLGGIALTAGS
                     ALGTPGHQPDVISISLGYCENELNGNMGGRDALDSIFAVAAGAGISVLVSAGDQGSTG
                     CRTAEAGSEELTALPMRAVSLPASSPYVTAVGGTNLGLTPKNKIKVEVVWNNSVIIPW
                     GGGGGGSIITPRTPWWQAGISSYGHGRKVPDIAALADIYPGYAFFCTAASCKPERNVV
                     YGWSKVGGTSAAAPLTAAGIALVNQYAAKQGQPPLGFLNPLLYDLGASAKSRASSFND
                     VTIGNNDIGIALPPEAGGGVPVGCCQAKPGYDWASGWGSLKLPSFAKAAVAAGN"
BASE COUNT          806 a         1834 c         1913 g          838 t
ORIGIN      
        1 cgtgttcttg gcgaggatcg tgatcccctt ctcgcgctcg aggtccatcg agtccatcac
       61 ccgctcggcg acgtcctggc ctttgcggaa ggtgccggtc tgccacagca tcgcatcgac
      121 cagggtcgtc ttgccgtggt cgacgtgggc gatgatggcg atatttcgca ggtcgtctcg
      181 ggtttcagtg ctcatgggcg aattgaagag ggtagggaac ggagggcgcg ggcatgtaaa
      241 cggcccctgg caagacgaag cccgccttgc ggcgggcttc ggtactgctg aagcgtcaga
      301 gaagctagtt ctgctgaacg ctcgccgcgg ccgggccctt gggcccttct tcggactcgt
      361 aggtcacctt ggcgccctcg gtcaggctct tgaagccctc acccaggatc gcgctgtggt
      421 ggacgaagac gtccttcgag ccgtcatcgg gagtgataaa cccgtagccc ttgtcgtcgc
      481 tgaaccactt gacagttcca gtcggcatgc attgcctcca catcatgtgc tgcgaacgcg
      541 gaggatgctc gtgaagcaac gactgcgagc gctacacaac ttttttgccg gtcatatatc
      601 cggcccttgc aagcgggacc gtagcaggat cgcggcgtcc agtacctata tttcggagat
      661 cgtgcgaccc gtgcgcaagc gcctcgcggc gcggactgcg acgtttgtcg ccctggcctt
      721 ttcggcgctg gtgcccgtgg ccgcggcgaa tgccgccctg gtgccgctgc agcacggccc
      781 cggcactccc ctgcgcgacg gcaagcgttt gatcttcgac gtccgcttcg agcgcggggc
      841 cgtcgccggg ctggacgaat tgcgcgccgc cggcgccgag atcgtccaca cgagccgccg
      901 ctaccagacc gtcaccgtgg cggtgaagcc cgcggacctg ccggcgctcg acggcgtcgc
      961 cagggtgggc ggggttaaag aggtagtgcg accgctggtg atgggggccg actgtggcgg
     1021 gatcctgcgc tcggagggcg attcgcagtt gctcgctccg aacgcgcgcg ccaacatcgg
     1081 cgtcgacggc accggggtaa ctgtcggcat cctctccgac tcctacgacc gcgacccgac
     1141 tgcgccgact cgcgccgtca ccgacgtcgc gaccggcgac ctgcccggcc ccggcagccc
     1201 ctgcggctcg accgctcccg tcaacgtgct cgacgactcg gtgcaagaga gcgacgaggg
     1261 gcgggcgatg gcccagatcg tgcgcgacct cgcacccggg gcctcgatca gcttcgccac
     1321 cgcgttcaaa ggcgaagcgg cttttgccgc ctcgattaaa gcgctggccc aggccggggc
     1381 gcgggtgatc accgacgacg tcgtctactt cgaagagccg ttcttccagg acggcccgat
     1441 cgccgtcgcg atcgacgaag tcgccgccgc cggggtcagc tacttctcgg ccgccggcaa
     1501 caacaacctg ttctcgggcg gcaacgaagt cggctcctgg gaggcgccgt tccgcaacgc
     1561 tgaaacctgc ccgccggggc tgccttccgg cgaagaacag tgcgccgact tcaatcccgg
     1621 gggcggcacc gacaccctgt tcaacttcag cgtgcccagc gaaggcgagc tgatcctcga
     1681 cctgcagtgg gccgaaccgc gcggcggcgt cgccaccgac ttcgatgcct acctgatcaa
     1741 ctccaaaggc caaatagtcg acagctccaa tgacgacaac atcggcggga cccagcggcc
     1801 ctacgagttg gtccgaactg aaaacaccgg tgaatcggca gtccctgtat ggcttgcgat
     1861 cacgcgcaaa gccggcagcg gcacgccgcg gttgaagtgg gtccagctcg gcaacgcggt
     1921 cccagccgcc acccagtacg aaacttccag cggcgacgtc ttcggcccga ccgtgttcgg
     1981 ccacgctgcc gccaagggcg cgatcgcggt cggcgcggtc cgcttcaaca cggccagcgc
     2041 gccggagacc ttctcctcgc gcggtccggt gaccaactac ttcggcccgg tcgtgggcgc
     2101 cggcccggcg gcgccgctgg ccctgccgga agtcgtctcc aagcccgaca tcgccgccag
     2161 cgacggtggc gtcaacacct tcttcggcag cttcacggga gggttctggc gcttcttcgg
     2221 cacctccgcc gccgctcccc acgcggctgc ggtcgccgcg cttgccctgc aggccaaccc
     2281 gggcgcgacc ccgacccaga tccgcaacgc gctgacctcg accgcccgcc cggtcggtac
     2341 cttcggccca ctcgcggtcg gcgccgggct gatcgacgcg ggcgccgcgg tcaactcgct
     2401 ggcgctggcg ccgggcgtcg cggtgacggt gccgccggcg gcgcgcagcc gcgtgcgcca
     2461 gccgaccttc gagttcgaag ccagccggcc cgccacctac agctgcgcga tcgacggcgt
     2521 cgcgatgccc tgcgcctcgc cgttcacctg gccgcagccg ctcggcgacg gcccccactc
     2581 gctcgtcgtc ggcgccaccg acctggccgg ccgggtcggg agcagcgctg cggtcgggtt
     2641 cacgatcgac agcaaggcgc cgcagacgac gatcgtcaag aagccgccga aggtggtgcg
     2701 gacgagcaat ttcggcgccc gcgtcgtttt ccgcttccgc tccagcgagc ggggggcgcg
     2761 cttccgctgc aagatcgaca agaccggcta ccgcaagtgc ggcaagcgcc tctccaagcg
     2821 ctttacgctt ggcgcccacg tgctgcgggt gaaagcggct gacgcggtcg gcaacctcga
     2881 cccgagcccc gccgtctacc gcttccgcgt cgtccgtaag ccttagtgct gctgcgccga
     2941 ttggcggcgc atgaccagat ggccggcgcc gagcagggcg agcagcacgc cggcgctgat
     3001 gaagacgagc gggtcggtca gcgagaggcc ttcgagccgg gtgccgaagt agaccgacag
     3061 ggcggtgatc ggcagatagc cgaccgcggt cgtccagatg aagcgccaga gcggcacccg
     3121 ggcggcgcca gcggcgtagg agacgatgct gaaggggagg atcgggatca gccgcatcgc
     3181 cagcagcagg gtgacgccgc cgcgctcgat tcgcgcctcg atccgctcga agcgctcggc
     3241 cccgagccag cggtcgagca gcgggcgcgc caccgaccct ccgatcagcc agcagaggat
     3301 cccggagacg acccagccga acatcgtcag gaccagcgcc gggaagaacc cgtaggcgaa
     3361 gccagcggcg aagtcgacga tctcggccgg gtagaagacg acggtgtgga tcagcgccag
     3421 gccgaagatc agcagcgggc cccagacacc gaggtcgtcg atctccttgc gcaccgcttc
     3481 gtggtcgcct tcgatcgcgt agcggaccga gtcccgcagg tcggggatcg cgaggaagag
     3541 cgccgccagc agcgcgattc cgcccagcgt gatcgcgatc tcggtcagcg gcaggcgggg
     3601 acgctcacgt gactcccctg cctgggacgt ctcgtgttgc tgctccccgg tcgccatcgc
     3661 gcgccagggt ctcacatcgg tgtcgtggct tcggtggccg ctcagttacc ggcggcgacc
     3721 gcggccttgg cgaagctggg cagcttcagc gagccccagc cggaggccca gtcgtaaccc
     3781 ggtttggcct ggcagcaccc gaccgggacg ccgccaccgg cttccggcgg cagggcgatg
     3841 ccgatgtcgt tgttgccgat cgtcacgtcg ttgaacgagc tggcgcgcga cttggcgctg
     3901 gcgccgaggt cgtagagcag cgggttgagg aacccaagtg gcggctggcc ttgtttggcc
     3961 gcgtactggt tgaccagagc gatcccggcc gcggtcagcg gcgctgcggc gctggtcccg
     4021 ccgaccttgc tccagccgta gacaacgttg cgctccggtt tgcaggaggc ggcggtgcag
     4081 aagaaggcgt agcccgggta gatatcggcg agcgcggcga tgtcggggac cttgcggccg
     4141 tgtccataac tggagatgcc ggcctgccac cagggggtgc gcggggtgat gatgctgccg
     4201 cctccgccgc cgccccaggg gatgatcacc gagttgttcc agacgacctc gaccttgatc
     4261 ttgttcttcg gggtgaggcc gaggttggtg ccgccgaccg cggtcacgta gggcgaggag
     4321 gcgggcaggc tgaccgcgcg catcggcagg gccgtgagtt cttcgctgcc cgcttcggcg
     4381 gtccggcagc cggttgagcc ctggtcgccg gcggagacga ggacggagat gccggcgccg
     4441 gcggcaacgg cgaagatcga gtcgagcgcg tcgcggccac ccatgttgcc gttcagttcg
     4501 ttctcgcagt agccgagtga gatcgagatc acgtcgggct ggtggccggg ggtaccgagc
     4561 gccgagccgg cggtgagggc aatgccgccg agcgactcca ggccctcgta gacgtagatc
     4621 ttgtcgagct tgggggcgcc gacggccagc atcgagaggt cgagcgtggt ctcgtcttcg
     4681 ccgggcagcg gtttcgggac ctcgaccggc acgactttga tcggcggcgg cttggcgatc
     4741 ccgaagcact tggcgaaggc ggcgatgtcg ctgtgcttga agccgccggt ctcgaccacg
     4801 gcgacggtct ggccctggcc cttgaagcct ttggcgtgca ggtcggagtg gccgtaggcg
     4861 gtcaggtact ggttcggggt gaacggcggc aggccgggtt cgccgccgct gctgccggcg
     4921 gggcagccgc cggcggtgcc gctgtgcagg aggatcgagc ggtagggctt gccgaagtcc
     4981 gccgccgtga tttctttaga ggccgaggcc gcagcctcgg tcggcaccgc ggggccggcc
     5041 aggggctgca cgaccggcgc ggcgctgacc accgagatcc ccgtaacggc cccctcgagt
     5101 ccggcgggga tgttgcggtc gagcccggtg accgaggccg cggcgccgtc ggcgggcggc
     5161 agcagacgcg aggcctgcgc gccggtcatc gacacgatcg cgtgggtgcc ggagccgatc
     5221 accttggcgt gcagcccgcg ggcggcgaac cagcgcacca cctgcttttt cgtctgcggc
     5281 ttggcgccga agcggttgac cagctgggcg accgtggcgt agtcccggta gcgcggcgag
     5341 gcggggtcgg agacggtgcg cacgaagttg ttgaggccgc gcgggtgctg t
//
LOCUS       DASRIG010000101         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_822742, whole genome shotgun sequence.
ACCESSION   DASRIG010000101 DASRIG010000000
VERSION     DASRIG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822742"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="ruvB"
                     /locus_tag="VFB52_02775"
     CDS             <1..996
                     /gene="ruvB"
                     /locus_tag="VFB52_02775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZO05283.1"
                     /translation="ARADVPDEDLDRSLRPPTIDDFVNQAQVTEQLAIFIEAAKRRGE
                     ALDHVLLAGPPGLGKTSLAHIVAAEMGVPMVQTAGPALERKADVASFLTALEPGSVFF
                     IDEIHRLGRAVEETLYPAMEDGELPVVLGQGAGARTVTLPLPPFTLIGATTRAGLLTT
                     PLRDRFGVSHRLEHYSPEHLALIVERSAEILDVEIDAGGAATIASRSRGTPRVANRLL
                     KRVRDFAEVKGTGEIGTAVAADALEMLEVDAAGLDRSDRSLLDTIASKFGGGPVGLST
                     LAIAIGEEQDTIEDVLEPYLLQQGLLKRTPRGRVLTQRAYDHLGMPAPEGAASLF"
     gene            1231..1509
                     /locus_tag="VFB52_02780"
     CDS             1231..1509
                     /locus_tag="VFB52_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05284.1"
                     /translation="MTGLTDEDVIGRPVREVLGLDWIDQGDGGQTADSDGDGVTDPIE
                     TSLEWGVRSLGKRVSVNAEGDLPAKAVADVFPAYDDDGGLLLVLTPEG"
     gene            1516..>3197
                     /gene="secD"
                     /locus_tag="VFB52_02785"
     CDS             1516..>3197
                     /gene="secD"
                     /locus_tag="VFB52_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZO05285.1"
                     /translation="MGSRRRHLFVLLFVGLLLVISGLVIANNSTKLGLDLKGGLELVY
                     EGQPTGQVEEVSGEDIERAIEIIRQRIDELGVSEPEVSRLGSTEISVSLPDVTDARAA
                     VEQVGTTAQLYFYDWEPNLLGPEKAIAGRPGQPNQKAVEESQKRWREAGRNPNKYENE
                     VLIFAGAYPTAYEAALLASEQEPVQNCENCSANQPRFYLFDKDEPHAMLAGPEIRKQD
                     LYLSPTGEKRPKDGLVVEVPVGTIVVSELPTNREGEALSEDEADPGWYAIKDDPALSG
                     TDITDPEQTYGQFAGEPQVSFKFTDQGRESFQEVTRRIAQRGAASAIGPVGNEEAAQT
                     SGHFAVVLDNEVKTRPIINFAENPDGIDGRTGAQISGGFQSVTEAQDLATTLQIGALP
                     IDLRLISQTQVSATLGSQALDEGIKAGIIGVALAILFLILYYRFLGFIASIALVAYGI
                     IFFALIDLIPITLTLPGIAGLVLTIGVAADSNIVIFERIKEEVRAGRSMSSAIATGYK
                     RGISTIIDANVITLLTAFILFVLATAGVKGFAFTLGVGTIVSLLTAVVFTQAL"
BASE COUNT          559 a         1116 c         1028 g          494 t
ORIGIN      
        1 gcccgcgctg acgtgcccga cgaggacctc gaccgcagcc tgcggccgcc gacgatcgac
       61 gacttcgtca accaggccca ggtcaccgag cagctggcga tcttcatcga ggcggcgaag
      121 cggcgtgggg aggcgctcga ccacgtcctg ctcgccggcc ccccggggct cggcaagacc
      181 tcgctcgccc acatcgtcgc cgccgagatg ggcgtgccga tggtgcagac cgccggtccg
      241 gcgctcgagc gcaaggcgga cgtcgcctct ttcctgaccg cgctggagcc gggcagcgtc
      301 ttcttcatcg acgagatcca ccggctcggc cgcgccgttg aggagaccct ctacccggcg
      361 atggaggacg gcgagctgcc ggtcgtgctc ggccagggcg cgggcgcgcg cacggtgacg
      421 ctgccgctgc ccccgttcac gctgatcggg gccaccaccc gcgccggcct gctgaccacg
      481 ccgttgcgcg accgtttcgg cgtctcccac cggctcgagc actactcgcc cgagcacctg
      541 gcgctgatcg tcgagcgctc cgccgagatc ctcgacgttg agatcgacgc cggcggcgcg
      601 gccacgatcg cctctcgctc acgtggcacg ccgcgggtcg cgaaccggct gctgaagcgg
      661 gtccgcgact tcgccgaggt gaaggggacg ggggagatcg gcaccgccgt cgccgccgac
      721 gcgctggaga tgctcgaggt cgacgccgca ggtcttgacc ggtccgatcg ctccctgctc
      781 gacacgatcg cgagcaagtt cggcggcggc ccggttggcc tctcgacgct ggcgatcgcg
      841 atcggtgagg agcaggacac gatcgaggac gtgctcgagc catatctgct ccagcagggc
      901 ctgctgaaga ggaccccgcg gggacgcgtg ctcacgcagc gcgcctacga ccatctcggc
      961 atgcccgcgc ccgaaggtgc ggccagcctc ttctaggcgc gctgctctag cctgtcgcgc
     1021 atgccgtact tcatctgccc taactgcggg aaccgcgagg cgagccagga ccgcaccgcc
     1081 ggcttcagca ctcgccccaa gggctgctcc aaatgtggat tcggattcgt attcgaatta
     1141 ctcgacgatt attatccggc tcctaacgcg gcgtttttcg tttgcgatag gcaggaacgg
     1201 gtgatcgacg cagggaaagg gagctacgag ttgaccggtc tcacggacga ggacgtgatt
     1261 ggacgccccg tgcgcgaggt gctgggcctc gactggatcg atcaaggcga cggtggccag
     1321 accgccgaca gcgacggcga cggggtcacc gatccgatcg agacttcgct cgagtggggc
     1381 gtgcgctcac tcggcaagcg ggtctccgtg aacgccgagg gcgacctccc ggcgaaggcg
     1441 gtggccgacg tcttccccgc ctacgacgac gacggcgggc tgctgctcgt cctcactccg
     1501 gagggctagg caaccgtggg ctcgaggcgc agacacctgt ttgtcctgct cttcgtcggc
     1561 ctgctgctgg tgatctccgg gctcgtcatc gccaacaact cgaccaagct cggcctcgac
     1621 ctcaagggcg gcctggagct cgtctacgaa ggccagccga ccggccaggt cgaggaggtc
     1681 agcggcgagg acatcgaacg ggcgatcgag atcatccgcc agcggatcga cgagctcggt
     1741 gtctccgagc cagaggtctc gcgcctcggt tccaccgaga tctcggtcag cctgccggac
     1801 gtcaccgacg cccgcgccgc ggtcgaacag gtcggcacca ccgcgcagct ctacttctac
     1861 gactgggagc cgaacctgct cgggcccgag aaggcgatcg ccggccggcc cggacagccg
     1921 aaccagaaag ccgtagaaga atcacaaaaa cgttggcgcg aagcgggtcg taacccgaac
     1981 aaatacgaaa acgaagttct gatcttcgcc ggcgcctacc cgaccgccta cgaagcggcg
     2041 ctgctggcct ctgaacagga accggtccag aactgcgaaa actgctccgc caaccaaccg
     2101 cgcttctacc tcttcgacaa agacgagccg cacgcgatgc tggccggtcc ggagatccgc
     2161 aaacaggacc tctatctcag tccgaccggc gaaaaacggc caaaagacgg gctcgtggtc
     2221 gaagtgcccg tcggcacgat cgtcgtctcc gaactgccga ccaaccggga aggcgaagcg
     2281 ctctccgagg acgaggcgga ccccggctgg tacgcgatca aagacgaccc ggcgctgagc
     2341 ggcaccgaca tcaccgaccc cgaacagacc tacggccagt tcgccggcga accgcaggtc
     2401 tcgttcaaat tcaccgacca gggtcgcgag tccttccagg aagtcacccg ccggatcgct
     2461 cagcgcggcg ccgcttcggc gatcggcccg gtcggcaacg aagaagcggc ccagacctcc
     2521 ggccacttcg cggtggtcct cgacaacgaa gtcaaaaccc ggccgatcat caacttcgcc
     2581 gaaaacccag atgggatcga cgggcgcacc ggcgctcaga tctccggcgg attccagagc
     2641 gtcaccgagg cgcaggacct ggcgacgacc ctgcagattg gcgccctccc gatcgacctg
     2701 cgcctgatca gccagaccca ggtctcggcg acccttggct cgcaggcgct cgacgagggc
     2761 atcaaagcgg ggatcatcgg cgtcgccctc gcgatcctct tcctgatcct ctactaccgc
     2821 ttcctcggct tcatcgcttc gatcgcgctg gtcgcctacg ggatcatctt tttcgccctg
     2881 atcgacctga tcccgatcac cctgacgctg ccggggatcg cgggcctggt gctgacgatc
     2941 ggagtcgcag cggactcgaa catcgtcatc ttcgagcgaa taaaggagga ggtgcgggct
     3001 ggacgctcga tgtcgagcgc gatcgcgact ggctacaaac gcgggatcag cacgatcatc
     3061 gacgcgaacg tgatcaccct gctgaccgcg ttcatcctct tcgtgctggc gaccgccggg
     3121 gtcaagggct tcgccttcac cctcggggtc ggcacgatcg tctcgctgct gaccgcggtt
     3181 gtgttcacgc aggcgct
//
LOCUS       DASRIG010000102         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3707234, whole genome shotgun sequence.
ACCESSION   DASRIG010000102 DASRIG010000000
VERSION     DASRIG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707234"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFB52_02790"
     CDS             <1..676
                     /locus_tag="VFB52_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05286.1"
                     /translation="CGTATSAVAKASPAIAGQVGKRLLVGNKFRDAVTLAGGYSPTGS
                     ITFAIYAPGTSGCAKPSFVNTVAVNGNGTYRSDPFVAKRAGNYRFVATYSGDASNRTA
                     TEPCDGANQLARVQKRTPKLRPLARLVGGQQISIRARLSAASSPTGTIAFRLYGPNDK
                     RCSSRPAFAGSLRVKANGAFPLAEYIATKPGTYRLALSYSGDPRNRSSKVSCAASQAI
                     VISRTG"
     gene            847..2325
                     /locus_tag="VFB52_02795"
     CDS             847..2325
                     /locus_tag="VFB52_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZO05287.1"
                     /translation="MSGLSAETEGAVVIAGGGPTGLMLAGELALAGVACTVLERRQGQ
                     ELDGSRAGGFHTRTIEILDQRGIAERFLSEGQTHAVALFADAALDISDLPTRHPYTLA
                     LWQKHIERILAGWVGELGVPVHYGQEVAGFAQDDDGVDVRLADGSSLRTRYLVGCDGG
                     RSAIRKAAGIEFAGWDPTLSALIAEVEMAEQPEPGVRHSDFGISGIGPTGEGESFRVV
                     VTERQLETGEPTVDELREALVAVYGSDFGLHSPIWISRFTDATRQAADYREGRVLLAG
                     DSAHIHYPAGGQGIQHGTQDAVNLGWKLAQVVNGTSPDSLLDTYRAERHPPTAAALQE
                     TLAATVLHRGDARTAAVRKMLTELATMEEPRRHLAARRVGLDIRYDLGEGHPLLGRRM
                     PDLDLVTGDGPLSAYSLLHRARPVLLDLGEPGAIEITPWADRVQLVEAEYSGAWELPV
                     VGAVAAPSAVLIRPDGHVAWVGDGTANGLADALTTWFGPPNP"
BASE COUNT          392 a          981 c          792 g          292 t
ORIGIN      
        1 ctgcgggacc gcgacctcgg cggtcgccaa agcgagcccg gcgatcgccg gccaggtcgg
       61 caaacgcctg ctggtcggca acaagttccg cgacgccgtc accctcgccg gcggctactc
      121 ccccaccggc agcatcacct tcgcgatcta cgcccccggg accagcggct gcgccaaacc
      181 gagcttcgtc aacaccgtcg cggtcaacgg caacggcacc taccgctccg accccttcgt
      241 cgccaaacgc gccggcaact accgcttcgt cgccacctac tccggcgacg cctccaaccg
      301 caccgcgacc gaaccgtgcg acggcgccaa ccagctcgcc cgcgtgcaga agcgcacccc
      361 gaagctgcga cccctcgccc gcctcgtcgg cggccagcag atctcgatcc gcgcccgcct
      421 ctccgccgcc tcctccccca ccggcacgat cgccttccgc ctctacggcc ccaacgacaa
      481 gcgctgcagc agccgccccg ccttcgccgg cagcctccgc gtcaaggcca acggcgcctt
      541 cccgctcgcc gagtacatcg ccaccaagcc cggcacctac cgcctcgccc ttagctactc
      601 cggcgacccg cgcaaccgca gctccaaggt cagctgcgcc gccagccagg caatcgtgat
      661 cagccggacc ggctgagccc tccgcgacgc ccggcccacg aaaaaggacc gctctcgcgg
      721 tccggaaaat cgggcagcaa cctcctgcca cacccaaaat atagagcaac gagggcttga
      781 atcaaggccc gcctcacctg taaaaaggcc cgccgcaggg cggaactttc gaaccggagg
      841 tggcagatga gtgggctgag cgcagagacc gagggtgcgg tcgtgatcgc gggtggaggg
      901 ccgaccgggc tgatgttggc gggcgagctg gcgctggcgg gcgtcgcctg caccgtgctc
      961 gagcggcgcc agggacagga gctagacggg tcgcgggccg ggggctttca cacgcgcacg
     1021 atcgagatcc tcgaccagcg cgggatcgcc gagcggttcc tcagcgaggg acagacgcac
     1081 gcggtcgcgc tcttcgccga cgccgccctc gacatcagcg acctccccac ccgccacccc
     1141 tacacgctgg cgctctggca gaagcacatc gaacggatcc tggccggctg ggtcggcgag
     1201 ctcggcgtgc cggtccacta cggccaggag gtcgccgggt tcgcgcagga cgacgacggc
     1261 gtcgacgttc gcctcgccga cggcagctcg ctgcgcaccc ggtacctggt cggctgcgac
     1321 ggcggtcgca gcgcgatccg caaagccgcg ggcatcgagt tcgccggctg ggacccgacc
     1381 ctgagcgcgc tgatcgccga ggtcgagatg gccgagcagc ccgagcccgg cgtccgccac
     1441 agcgacttcg gcatcagcgg catcggcccg accggggaag gcgagtcgtt ccgcgtggtc
     1501 gtcaccgagc ggcagctcga aaccggcgag ccgaccgtgg acgagctgcg tgaggcgctc
     1561 gtcgccgtgt acgggagcga cttcggactc cacagcccga tctggatctc ccggttcacc
     1621 gacgcgaccc gccaggccgc cgactatcgc gagggaaggg tgctgctcgc cggcgactcc
     1681 gcccacatcc actacccggc cggcggccag ggcatccagc acggcaccca ggacgcggtc
     1741 aacctcggct ggaagctggc ccaggtggtc aacgggacct cgcccgactc cctgctcgac
     1801 acctaccggg ccgagcgcca cccgcccacc gccgcggccc tgcaggaaac gttggccgcc
     1861 accgtcctgc accgcggcga cgcccgcacc gccgcggtcc gcaagatgct caccgagctg
     1921 gcgacgatgg aggagccgcg ccggcacctc gccgcccggc gagtcggcct cgacatccgc
     1981 tacgacctcg gcgagggcca tcccctgctc ggccgccgca tgcccgacct cgacctcgtc
     2041 accggcgacg gtccgctgag cgcatactcc ctgctccacc gggcccgccc ggtgctgctc
     2101 gacctcggcg aacccggcgc gatcgagatc accccctggg ccgaccgggt ccagctggtc
     2161 gaggcggagt actccggcgc ctgggagctc ccggtcgtcg gcgccgtcgc cgcccccagc
     2221 gccgtcctga tccggccgga cggccacgtc gcctgggtcg gcgacgggac cgccaacggc
     2281 ctcgccgacg ccctcaccac ctggttcggc ccaccaaacc cgtgaggtcg catttgtccc
     2341 cggtacgcgg caacaaatgc gacctcacac agagcggagc gcttccgtgg gggtgagccg
     2401 ggcggcgcgc acggccgggt aaaggccggc gagtccgccg agcaagcagg cggcgag
//
LOCUS       DASRIG010000103         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1615658, whole genome shotgun sequence.
ACCESSION   DASRIG010000103 DASRIG010000000
VERSION     DASRIG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615658"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFB52_02800"
     CDS             complement(<1..830)
                     /locus_tag="VFB52_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZO05288.1"
                     /translation="MARWVLGLVLLALLVPAAASAAEQDAPPKISETEAQEIADRDPV
                     VADQRRKNGELTDSVKMDDDDERWEVAYFADDHQVALVIVDAESGEVEEAWTGYQVAW
                     RMARGYSGAFGHKLNAPYVFIPLCLIFLIGLVDWRRLWRVANLDLLVLVGFGLSHIFF
                     NRAEIGVSVPLYYPVLVYLLARALWIGFRGWGQGLRPIWPTVWILIAALFLMGVRSGL
                     NMADAGVADIGYASVVGADRITHGEPIYGNFPDDVSQGDTYGPVTYYAYVPFELVFPW
                     G"
     gene            complement(830..2368)
                     /locus_tag="VFB52_02805"
     CDS             complement(830..2368)
                     /locus_tag="VFB52_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO05289.1"
                     /translation="MAASKKLVLTYVDSLRTDMLQRAVAEERAPTFGALLERGVFVPD
                     CVSSFPSVTPVACSEMVTGVGADQHWISGMNWYHRLERRYVEYGSSLEATRAFGIFRA
                     LYDLVYNMNLAHLSPEIETLFEQLDDAGARTACTPFLIYRGRHRHQVSLDGLMRLGVE
                     ASKLKFRHHTWGPADLFYGDLYASREVPCKSTSIPGNRDPYAACCVAELVREDHCDFI
                     LFSLPDNDNYSHKHGPEASVESIARADAAFAQVVEAGGGLDEFLAGHAVILLADHAQT
                     DVHRGLPLAELLGHEWSVLQPSDDRPELAQLAVSPTGRAAHVYLLPGEGERPDPDAVR
                     TRLSEIEGVELVCRLEGSDGTPLRRDEPGMPAAGGEWAVVERAGEAVRFRPAEPGVGE
                     ALADLRGGRWQLEGELDVLEARVEDGRLRSDTYPDPLARVFAALAAPHSGDFVVSLAE
                     GFEAVDWGGTSHAGGGSHGALRAGDSLGPLLFVGCGPESAEEKEQWTLRDVAPVVLEH
                     FGLN"
     gene            complement(2410..2814)
                     /locus_tag="VFB52_02810"
     CDS             complement(2410..2814)
                     /locus_tag="VFB52_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05290.1"
                     /translation="MDFLTELGIGIAIGMVAGTTGTHGGARGRMTLLAAVIGLVVGYL
                     LDDVVGAVLGMVGAAGACLIISDLVAGASRREGGGAAGLAFIVMLAALIVVAISLLLP
                     ILVILVVLALLWLAFTRHRRAQRKHAGLRVLR"
     gene            2869..4242
                     /locus_tag="VFB52_02815"
     CDS             2869..4242
                     /locus_tag="VFB52_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZO05291.1"
                     /translation="MAEVPQPPHRRRAHTSTRDLERYAGLFAERTKVMRSSAMRDLMA
                     ITARPEVISLAGGLPDTSTFPPQSFAAQMTRIAQESAAEALQYGPTEGFEETKDCILQ
                     VMAAEGMLPDPDDLIVTTGGQQAIDLITKTLVDPGDVVICEAPTYPGAVPVFCSYQAD
                     VIQIECDHDGMRIDLVEATLEQLRREGRRPKFIYSVPTFQNPAGVTMSLERRARLVEL
                     ARLHELLVVEDSPYGLLRYSGEQLPPLYQLDGGDFVIYLGTFSKILSPGIRLGWMVAP
                     PPVMEKVVLGKQAADLCSSTLTQFFVREYFAEGRWRQYIEELVEIYRGRRDTMLAALE
                     EHFPAEATWTEPEGGLFIWATLPDYIDTGDLLAKALRADVAFVPGQGAYVDGRGGSSM
                     RLNFSGVNEDEIREGIRRIGKTIDEQVELYGALTGEQRIPVPRPEAEPQHEENVVPFR
                     KAGEAGA"
     gene            4239..5174
                     /locus_tag="VFB52_02820"
     CDS             4239..5174
                     /locus_tag="VFB52_02820"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZO05292.1"
                     /translation="MKVAVLKGGRSLERGVSLRSGARVEDALERLDHEVLPIDVGSDL
                     VKRLVGERPDVAFVAMHGVGGEDGTAQELLEILGIPFTGPGAAACARCMDKSLAKHLI
                     REAGVPTPDWFAFNQAAFRELGAADALAQLERSLGFPLVVKPSRGGSSLGIKFAGSPA
                     EVPQALVSAFSYDDRVVLERFVDGRELAVSVLGDEALPVVEALPAEGDRYDFEARYAI
                     GRTRFVCPAELSDEEERAVIEAALGTYRALGCTGFARVDLILAADGPWVLEANAIPGL
                     TDTSLLPQAAEAAGMSFERLVERILDLALAPSGVS"
BASE COUNT          789 a         1855 c         1855 g          694 t
ORIGIN      
        1 ccccagggaa agaccagctc gaaggggacg taggcgtagt aggtcaccgg cccgtaggtg
       61 tcgccctggg agacgtcgtc ggggaagttg ccgtagatcg gttccccgtg ggtgatccgg
      121 tcggcgccga cgacgctcgc gtagccgatg tcggcgaccc cggcgtcggc catgttgagg
      181 ccgctgcgca cccccatcag gaagagcgcc gcgatcagga tccagacggt cggccagatc
      241 ggccgcagcc cctggcccca gccgcggaac ccgatccaca gcgcgcgggc cagcaggtag
      301 acgagcaccg ggtagtagag cggcaccgag accccgattt cggcgcggtt gaagaagatg
      361 tgcgagaggc cgaagccgac gaggaccagc aggtcgaggt tggcgacccg ccagagccgc
      421 cgccagtcga ccaagccgat caggaagatc aggcagagcg ggatgaagac gtagggcgcg
      481 ttcagcttgt gcccgaaggc gccggagtag ccgcgcgcca tccgccaagc gacctggtag
      541 ccggtccagg cctcctcgac ctcgccgctc tcggcgtcga cgatgaccag cgcgacctgg
      601 tggtcgtcgg cgaagtaggc gacctcccag cgctcgtcgt cgtcgtccat cttcacgctg
      661 tcggtcagct cgccgttctt gcggcgctgg tcggcgacca ccgggtcgcg gtcggcgatc
      721 tcctgcgctt ccgtttcaga gatcttcggg ggcgcgtcct gctcggcagc gctcgctgcg
      781 gccggaacca gcagcgccag gagaacgaga ccgagaaccc agcgcgccat cagttcagcc
      841 cgaagtgctc gaggacgacg ggggcgacgt cgcgcagcgt ccactgctcc ttctcctcgg
      901 ccgactcagg accgcagccg acgaagagca gcggcccgag cgagtcgccg gcgcgcaggg
      961 cgccgtggct gccaccgccg gcgtggctgg tcccgcccca gtcgaccgcc tcgaagccct
     1021 cggcgagcga gacgacgaag tcgcccgagt gcggcgcggc gagcgcggcg aagacccggg
     1081 cgagcgggtc ggggtaggtg tcgctgcgca agcggccgtc ctcgacccgc gcctcgagca
     1141 cgtcgagctc gccctccagc tgccagcgcc cgccgcgcag gtcggcgagc gcctcgccga
     1201 cgccgggctc ggccgggcgg aagcggaccg cctcaccggc ccgttccacg accgcccact
     1261 cgccgccggc ggccggcatc cccggctcgt cgcgccgcag cggtgtgccg tcgctgccct
     1321 ccagccggca gaccagctcg accccctcga tctcgctcag ccgggtgcgc acggcgtccg
     1381 ggtcggggcg ctcgccctcc cccggcagca ggtagacgtg ggcggcgcgg ccggtcgggc
     1441 tgaccgcgag ctgggccagc tccgggcggt cgtcggaggg ctgcaggacg gaccactcgt
     1501 ggccgagcag ctcggccagt ggcaagccgc ggtgcacgtc ggtctgggcg tggtcggcga
     1561 gcaggatcac cgcgtggccg gcgaggaact cgtcgaggcc gccacccgcc tcgaccacct
     1621 gcgcgaacgc cgcgtcggcc ctggcaatcg actcgacgct cgcctccggc ccgtgcttgt
     1681 gcgagtagtt gtcgttgtcg ggcagcgaga agaggatgaa gtcgcagtgg tcctcgcgga
     1741 ccagctcggc gacgcagcag gccgcgtagg ggtcgcggtt accggggatc gaggtcgact
     1801 tgcaggggac ctcgcggctg gcgtagaggt cgccgtagaa gaggtccgcc gggccccagg
     1861 tgtggtggcg gaacttcagt ttgctcgcct cgactccgag gcgcatcagc ccgtcgagcg
     1921 agacctggtg gcggtggcgg ccgcggtaga tcaggaacgg cgtgcaggcg gtgcgggcgc
     1981 cggcgtcgtc gagctgctcg aacagggtct cgatctccgg gctcaggtgc gccaggttca
     2041 tgttgtagac gaggtcgtag agcgcccgga agatcccgaa ggcgcgcgtc gcctccagcg
     2101 aggagccgta ctcgacgtag cgccgctcga gccggtggta ccagttcatg ccgctgatcc
     2161 agtgctggtc ggcaccgacg ccggtgacca tctccgagca ggcgaccggg gtcaccgagg
     2221 ggaagctgga gacgcagtcg gggacgaaga cgccgcgctc gagcagcgcc ccgaaggtcg
     2281 gcgcgcgttc ctcggccacg gcgcgctgaa gcatgtcggt gcgcagcgag tcgacgtagg
     2341 tgaggaccag cttcttgctc gcggccatcg ctcgaatgat gctcgaccgc gccgctcggc
     2401 ccagcgcggc tagcgcagga cgcgcagccc ggcgtgtttg cgctgggcgc ggcggtggcg
     2461 cgtgaaggcc agccagagca gggcgaggac gacgaggatc accaggatcg gcagcaacag
     2521 cgagatcgca accacgatca aggcagccag catcacgatg aaggcgagcc ccgcggcgcc
     2581 gccgccctcg cggcggctgg ccccggcgac gaggtcgctg atgatcagac aggcgccggc
     2641 ggcaccgacc atccccagca ccgcgccgac cacgtcgtcg agcaggtagc cgacgaccag
     2701 gccgatcacc gcggcgagca gggtcatccg cccacgggcg ccgccgtgcg tgccggtcgt
     2761 gccggcgacc atgccgatcg cgatgccgat ccccagctcg gtcagaaagt ccacgcccga
     2821 aaacctaacc acccgagcgc ccgcgtgtcg atccggcaca cttcctggat ggccgaagtc
     2881 ccgcaaccgc cgcatcggcg ccgcgcccac accagcaccc gcgacctcga gcgctacgcc
     2941 gggctgttcg ccgagcggac caaggtgatg cgctcctcgg cgatgcgcga cctgatggcg
     3001 atcaccgcgc gaccggaagt gatctcgctc gccggtggcc tcccggacac ctcgaccttc
     3061 ccgccgcaga gcttcgccgc gcagatgacc cggatcgccc aggagtccgc tgcggaggcg
     3121 ctgcagtacg gcccgaccga gggcttcgag gagaccaaag actgcatcct gcaggtgatg
     3181 gccgcggagg gcatgctgcc cgaccccgac gacctgatcg tcaccaccgg cggccagcag
     3241 gcgatcgacc tgatcacgaa gacgctggtc gatcccggcg acgtggtgat ctgcgaggcg
     3301 ccgacctacc ccggcgcggt cccggtcttc tgcagctacc aggccgacgt gatccagatc
     3361 gagtgcgacc acgacgggat gcggatcgac ctggtcgaag cgacgctgga gcagctgcgg
     3421 cgcgaaggcc ggcggccgaa gttcatctac tcggtgccga cattccaaaa ccccgcgggg
     3481 gtgacgatga gcctcgagcg ccgggcccgg ctggtcgaac tggcgcgcct gcacgagctg
     3541 ctcgtggtcg aggacagccc ctacgggctg ctccgctaca gcggcgagca gctgccgccg
     3601 ctctaccaac tcgacggcgg cgacttcgtg atctacctcg gcaccttctc gaagatcctc
     3661 tcgccgggga tccggctcgg ctggatggtg gcgccgccgc cggtgatgga gaaagtcgtg
     3721 ctcggcaagc aggccgccga cctctgcagc tcgaccctga cccagttctt cgtccgcgag
     3781 tacttcgccg aagggcgctg gcgccagtac atcgaggagc tggtcgagat ctaccgcggc
     3841 cggcgcgaca cgatgctggc ggcgctggag gagcacttcc ccgccgaggc gacctggacc
     3901 gaacccgagg gcggcctgtt catctgggcg accctgcccg actacatcga caccggcgac
     3961 ctgctggcga aggcgctgcg cgcggacgtc gccttcgtcc ccggccaggg cgcctacgtc
     4021 gacgggcggg gcggcagctc gatgcggctc aacttctccg gcgtcaacga ggacgagatc
     4081 cgcgagggga tccggcggat cggcaagacg atcgacgagc aggtcgagct ctacggcgcg
     4141 ctgaccggcg aacagcggat cccggtgccg cgccccgagg ccgagccgca gcacgaggag
     4201 aacgtggtcc cgttcaggaa ggccggcgaa gcgggcgcgt gaaggtcgcc gtcctcaaag
     4261 gcgggcgatc gctggagcgc ggcgtctcgc tgcgctcggg ggcacgggtc gaggacgcgc
     4321 tcgaacggct cgatcacgag gtgctgccga tcgacgtcgg ctccgacctg gtcaagcgcc
     4381 tggtcggcga gcgccccgac gtcgccttcg tcgcgatgca cggggtcggc ggtgaggacg
     4441 gcaccgccca ggagctgctg gagattctcg gcatcccctt caccggtccc ggcgccgccg
     4501 cctgcgcgcg ctgcatggac aagtcgctgg ccaagcacct gatccgcgag gcgggcgtgc
     4561 cgacccccga ctggttcgcc ttcaaccagg ccgccttccg cgaactcggc gccgccgacg
     4621 cgctcgccca gctcgagcgc agcctcggct tccccctggt cgtgaagccg agccgcggcg
     4681 gctcttcgct cgggatcaag ttcgccggct cccccgccga ggtgccgcag gcgctcgtct
     4741 ccgccttcag ctacgacgat cgggtcgtgc tcgagcgctt cgtcgacggg cgcgagctcg
     4801 cggtcagcgt gctcggcgac gaggcgctgc cggtggtcga ggcgctgccc gccgagggcg
     4861 accgctacga cttcgaggcc cgctacgcga tcggccgcac ccgcttcgtc tgccccgccg
     4921 agctcagcga cgaggaggag cgggccgtga tcgaggcggc gctggggacc taccgggcgc
     4981 tcggctgcac cggcttcgcc cgcgtcgacc tgatcctcgc cgccgatggc ccctgggtgc
     5041 tcgaggccaa cgcgatcccc ggcctcaccg acaccagcct cctcccccag gccgccgagg
     5101 cggcggggat gtcgttcgag cgcctggtcg agcggatcct cgacctcgcc cttgccccgt
     5161 cgggcgtttc ctaagttcgc agcccctagg aat
//
LOCUS       DASRIG010000104         7739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2925962, whole genome shotgun sequence.
ACCESSION   DASRIG010000104 DASRIG010000000
VERSION     DASRIG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925962"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFB52_02825"
     CDS             <1..577
                     /locus_tag="VFB52_02825"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05293.1"
                     /translation="QEARRREVLDAAVAVFSEQGYRAASMADIAARLGMGKASLYHYF
                     GSKEEVLIELYEEVLRENVIAARRIAEGAGGALELLTEMVADRVAYTCRNQDLLRVFF
                     EEEAELPARQRARLISVRHEYEETLLGVVAAGEAAGELRLPTNPVIFVNTLLGAANWT
                     YKWYSPAGDLSPEQLGDEIAGALLNGLATGR"
     gene            complement(552..1115)
                     /locus_tag="VFB52_02830"
     CDS             complement(552..1115)
                     /locus_tag="VFB52_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO05294.1"
                     /translation="MARIPLAEETDLDGFAKELHDAAEAGDWATQHVARVFAAHPDFL
                     EQYLTFYYPWHSNIEAAEDTARLSPRTKELVRLRIAILNGCVTCQSARLAPDHVPEQQ
                     ATGIWAEDFAANPEYSPAERAAVEFAERMALDHHNIGDVDVAALRAHFDDDQILELMM
                     MAGQYIGFGRCLAILQLETVTCPLPAR"
     gene            1278..2744
                     /locus_tag="VFB52_02835"
     CDS             1278..2744
                     /locus_tag="VFB52_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05295.1"
                     /translation="MATIEFVDQTLRDGQQSLWGMRMRAYQATPALPHLNRTGFRVAD
                     LTGGTFFVVMLRESGDDPWAALDHIVRGLPDVEHRAGMRPVAVGSFGFVPDEMLDLWV
                     ATLVKHGIHSHWLFDCLYDMPVMEAKAKAIRRVGGQVVPAIMYGLTDLHTDEFFADRA
                     REMASWEGVETIYVEDAPGVLTPERAATLIPALQAAVGDVRLELHVHNTTGLAPLVYA
                     EGIKAGIEIIHTCSLPLANGPSLPSTEAMVEIVEELGHDHNLDKSQLEPVADHFYAEA
                     QRMGWETGVPNEYRLLPYRHQLPGGMTGTLKNQLNEYGMPEKFPQVVDEIVRVREELG
                     QPVMATPFSQFVGIQAVLNVVMPERYQVVPDEVIHYSLGHYGPLMRPVEPEVADKIFA
                     QPRAKDLEKWERDQTPLSEFRKRLGQNLSDEELLLRYFCPEEQVDKMLAGPPLPTAPH
                     VHGNGSLDQVTRLIESASEAKHLSLRQPGISLDLRRED"
     gene            complement(2806..3603)
                     /locus_tag="VFB52_02840"
     CDS             complement(2806..3603)
                     /locus_tag="VFB52_02840"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HZO05296.1"
                     /translation="MDKAALGASYSQPIGTGSLYGPPPYLYRGVEDCFIVYEAERDGV
                     EALLPPGAEIADEVPTCIAWARWVPFSTFGQYHEAYIMIRARVDGEVYLYQPFIFVDN
                     EVPLGAGREIWGYAKKMAVFERNWGGGDTPYGEQMVFTVQRPKGQPIMTATIVCDRLA
                     DPAELEDTPVLSTRVIPNSEQGKPPSVAELVRLDVPATLHTAPDGTPKLWAGRGSLSL
                     PTVSSIDPWHLLRPTKVVEAYFGVYDFDLPHGRVIRNYLEDDEIVWA"
     gene            complement(3703..4464)
                     /locus_tag="VFB52_02845"
     CDS             complement(3703..4464)
                     /locus_tag="VFB52_02845"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZO05297.1"
                     /translation="MSGEGRLAGKIGLVTGAGSGIGRATAQRLAAEGAEVAVVDVVGA
                     EEVAAQIEADGGSALGITTDVADAEAVRHMADTAIDCFGRVDALVNNAGILDDYLPAA
                     DTPDEVWDRVLGVNLKGVFHTSRALIPQMLENGGGAIVNVASTAALVGGNGGAAYTTS
                     KHAIVGFTRQLCFDYALSGIRANTVCPGAVETGMTKEIFAAGDAEVMKAVRSAPIGRW
                     AQPDELASVIAFLCSDDAAFVHGATYVVDGGFSVK"
     gene            complement(4461..5207)
                     /locus_tag="VFB52_02850"
     CDS             complement(4461..5207)
                     /locus_tag="VFB52_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05298.1"
                     /translation="MSICVTGGSSGLGRAIAERFAHDGNDVIVNYHANDEAAEEAVAG
                     IEAAGGVPHLVKADVGTPEGVHAVIDAVREAGVEQLEQLDQLVHCAAKAVRGPIVDAD
                     PTELRESIVVNGLALVDLVREAGPLLGRGSNVFYLSSRGARFVIAEYGALGSAKALGE
                     HVVRYLAKELAPRGIRVNTIAPGGVDTAALRVMFPDTYEERLAAAAAANPMGRGLEIG
                     EAAALVERLCDPELEMLTGQTITLDGGISL"
     gene            5371..6558
                     /locus_tag="VFB52_02855"
     CDS             5371..6558
                     /locus_tag="VFB52_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZO05299.1"
                     /translation="MIDFELDPELLELRERVREFIREVAIPNEPRDVGTHGTDEGLRA
                     ELQEQAREWGVFAPQISTELGGLGLDHRGTAVILEESGYSLLGAQALNCAAPDEGTIH
                     MLDLIANPEQRERYLVPLAAGTARSCFSMTEPAPGAGSDPSMLLTTAEKTDSGWVING
                     RKWFITGAEGAAFTVCMAKTGEGATMFLVDADNPGFQIDRVIEATDRAFPGGHGEVTF
                     ENCEVGEDAVLGEVGEGFRYAQVRLAPARLTHCERWLGTARRAFDISLDYVNEREAFG
                     SKLGELGMVQEKIARSVMDIESSRAMIWRCAWALDRGEPAKHESSVAKAFVAEAVNRV
                     VDRAVQVCGATGISGDTLLARMMNELRPFRIYDGPTETHLWSIARRTLRGHAARTAQA
                     PSP"
     gene            6555..7637
                     /locus_tag="VFB52_02860"
     CDS             6555..7637
                     /locus_tag="VFB52_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZO05300.1"
                     /translation="MNAAGASSGDVVHTPAEAAAAQLEPLVVLEPLEAFLDGAGLGSG
                     PLSAAPIGEGHSNVTYLIERGGEKLVLRRPPRGPLPPSAHDVLREARLLIALRPHGTP
                     VPEVLATCDDPEVIGAPFYLMTFVDGWVLAGDLPAPLAGAGAPTRIGEELVDNLVELH
                     TLGPSAEGLAGFGRPDGYLERQLKRFGGLLETNMTRPLPDLERVTEWLSANLPASPPP
                     TVVHGDYRLGNVMYAPDEPRLAAILDWEMATIGDPLADVGYMTATWAGRDDPRNSVAD
                     LSPVTRLPGFPSRDDLARRYAERSGRTIEALGWYQVLAIWKAAIFLEGNYKRFVAGTT
                     ADPYYASLEQGVPELAQAALARAQGA"
BASE COUNT         1139 a         2656 c         2796 g         1148 t
ORIGIN      
        1 ccaggaggcg cgtcgccgcg aggtgctcga cgccgcggtc gcggtcttct ccgagcaggg
       61 ctaccgggcg gcctcgatgg ccgacatcgc cgcccgcctc ggcatgggca aggcctcgct
      121 ctaccactac ttcggctcca aggaggaggt gttgatcgag ctctacgagg aggtcctgcg
      181 cgagaacgtg atcgcggcgc ggcggatcgc cgagggggcc ggcggcgcgc tcgagctgct
      241 caccgagatg gtcgccgacc gggtcgccta cacctgccgc aaccaggacc tgctgcgggt
      301 cttcttcgag gaggaggcgg agctgcccgc acgccagcgg gcgcggctga tcagcgtccg
      361 ccacgaatac gaggagacgc tgctcggcgt cgtcgccgcc ggcgaggcgg cgggcgagct
      421 gcgcctgccg acgaacccgg tgatcttcgt caacaccctg ctcggcgccg ccaactggac
      481 ctacaagtgg tactccccgg ccggcgacct cagccccgag cagctcgggg acgagatcgc
      541 cggggcgctg ctcaacgggc tggcaacggg caggtaaccg tctccagctg aaggatcgcc
      601 aggcagcggc cgaagccgat gtactggccg gccatcatca tcagctcgag gatctggtcg
      661 tcgtcgaagt gggcgcgcag ggcggcgacg tcgacgtcgc cgatgttgtg gtggtcgagc
      721 gccatccgtt cggcgaattc gaccgcggcg cgctcggccg ggctgtactc ggggttggcg
      781 gcgaagtctt cggcccagat cccggtcgcc tgttgctcgg ggacgtggtc gggggcgagg
      841 cgggccgact ggcaggtgac gcagccgttg aggatcgcga tccgcagccg caccagctcc
      901 ttcgtccgcg gcgagagccg cgccgtgtcc tcggcggctt cgatgttcga gtgccagggg
      961 taatagaagg tcaggtactg ctcgaggaag tccgggtgcg cggcgaacac ccgggcgacg
     1021 tgctgggtcg cccagtcgcc cgcttcggcc gcgtcgtgca gctccttggc gaagccgtcg
     1081 aggtccgtct cctcggccag cggaatccgt gccattgctg tctctcctct gttgcggttg
     1141 cccccgagtt cccaggaccg aacctagttc cggggcaggg ccgcgcgagc ttggtactgg
     1201 agggtgcttt gggggtcttc cagggcagcc gtagtgggcg ctggaagccg gcgccggggg
     1261 cctgattagc tgtgggcatg gccacgatcg agttcgtcga ccagaccctg cgcgacgggc
     1321 aacagagcct ctgggggatg cggatgcgcg cctaccaggc gacgccggcg ctgccccacc
     1381 tgaaccgcac cgggttccgg gtcgccgacc tgaccggcgg caccttcttc gtagtgatgc
     1441 tgcgcgagtc cggcgacgac ccctgggcgg cgctcgacca catcgtcagg ggcctgcccg
     1501 acgtcgagca ccgggccggg atgcggccgg tcgcggtcgg cagcttcggc ttcgtccccg
     1561 acgagatgct cgatctctgg gtcgcgaccc tggtcaagca cgggatccac tcgcactggc
     1621 tgttcgactg cctctacgac atgccggtga tggaagccaa ggcgaaggcg atccgccggg
     1681 tcggcggcca ggtcgtgccg gcgatcatgt acgggctcac cgacctgcac accgacgagt
     1741 tcttcgccga ccgggcgcgc gaaatggcca gctgggaggg cgtcgagacg atctacgtcg
     1801 aggatgcgcc gggggtgctg acgccggagc gtgccgccac cctgatcccc gccctgcagg
     1861 cggcggtcgg cgacgtgcgg ctggagctgc acgtccacaa cacgaccggg ctggcgccgc
     1921 tggtctacgc cgaggggatc aaggccggga tcgagatcat ccacacctgc tcgctgccgc
     1981 tcgccaacgg tccctcgctg ccctcgaccg aggcgatggt cgagatcgtc gaggagctcg
     2041 gccacgacca caacctcgac aaatcgcagc tcgagccggt cgccgaccac ttctacgcgg
     2101 aagcgcagcg gatgggctgg gagaccgggg tccccaacga gtaccggctg ctcccctacc
     2161 gccaccagct gcccggcggg atgacgggga cgctgaaaaa ccagctgaac gagtacggga
     2221 tgccggagaa attcccccag gtggtcgacg agatcgtgcg cgtgcgcgag gagctcggcc
     2281 agccggtgat ggcgaccccg ttctcgcagt tcgtcggcat ccaggcggtg ctgaacgtgg
     2341 tgatgccgga gcgctaccag gtggtccccg acgaggtgat ccactactcg ctcggccact
     2401 acgggccgct gatgcgcccg gtcgagcccg aggtggccga caagatcttc gcccagccgc
     2461 gcgccaagga cctggaaaag tgggagcgcg accagacgcc gctctcggag ttccgcaagc
     2521 ggctcggcca gaacctctcc gacgaggagc tgctcctccg ctacttctgc cccgaggagc
     2581 aggtcgacaa gatgctcgcc ggcccgcccc tgcccaccgc cccccacgtg cacggcaacg
     2641 gcagcctcga ccaggtcacc cgcctgatcg aaagcgcgag cgaggcgaag cacctctcgc
     2701 tgcgccagcc gggcatctcg ctggacctgc ggcgcgagga ctgacggatc acgactgtcg
     2761 acttatgcgg cctatagcgc gcataagtcg acactcgtgg acgagctaag cccagacgat
     2821 ctcgtcgtcc tccaggtagt tgcggatcac gcggccgtgc gggaggtcga agtcgtagac
     2881 gccgaagtag gcctcgacga ctttggtcgg gcggagcagg tgccaggggt cgatcgagga
     2941 gacggtcggg aggctgagcg agccgcggcc ggcccagagc ttcggggtgc cgtcgggggc
     3001 ggtgtgcagg gtggcgggga cgtcgaggcg gaccagctcg gcgaccgagg gcggcttgcc
     3061 ctgctcggag ttggggatca cgcgggtcga gaggacgggg gtgtcctcga gctcggccgg
     3121 gtcggcgagg cggtcgcaga cgatcgtggc ggtcatgatc ggctggccct tggggcgctg
     3181 gacggtgaag accatctgct ctccataggg ggtgtctccc ccgccccagt tgcgctcgaa
     3241 cacggccatc ttcttcgcgt agccccagat ctcgcggccg gcgccgagcg ggacctcgtt
     3301 gtcgacgaag atgaacggct ggtagaggta gacctcgccg tcgacccggg cgcggatcat
     3361 gatgtaggcc tcgtggtact ggccgaaggt tgagaagggc acccagcggg cccaggcgat
     3421 gcaggtcggg acctcgtcgg cgatctcggc gccgggcggc agcagcgcct cgaccccgtc
     3481 gcgctcggcc tcgtagacga tgaagcagtc ttcgacgccg cggtagaggt agggcggcgg
     3541 gccgtagaga ctgccggtcc cgatcggctg cgagtaggag gcgccaagcg ctgctttgtc
     3601 catggggggt tccctctccg tgtagttctc agtcccgcag tcgagcctag gcgcggtcgg
     3661 cacccctgcc ggcgttggat cgcggcgctg ttgtggaact cactacttga ccgagaagcc
     3721 gccgtcgacg acgtaggtgg cgccgtggac gaaggcggcg tcgtcgctgc agaggaaggc
     3781 gatcacgctg gcgagttcgt cgggctgggc ccagcggccg atcggggccg agcgcaccgc
     3841 cttcatcacc tcagcatcgc cggcggcgaa gatctccttc gtcatcccgg tctcgaccgc
     3901 gcccgggcag accgtgttgg cgcggatccc ggacaacgcg tagtcgaagc agagctggcg
     3961 ggtgaagccg acgatcgcgt gcttggaggt cgtgtaggcg gcgccgccgt tgccgccgac
     4021 cagggccgcg gtcgaggcga cgttgacgat cgcaccgccg ccgttctcca gcatctgcgg
     4081 gatcagcgcc cgtgaggtgt ggaagacgcc cttgaggttg acgccgagga cgcggtccca
     4141 gacctcgtcc ggggtgtccg ccgcgggcag gtagtcgtcg aggatgccgg cgttgttgac
     4201 cagggcgtcg acccggccga agcagtcgat cgctgtatcg gccatgtgcc tgaccgcctc
     4261 ggcgtcggcg acgtcggtgg tgatcccgag ggcgctgccg ccgtcggcct cgatctgcgc
     4321 cgcgacctcc tcggcgccga cgacgtcgac cacggcgacc tccgcccctt cggcggcgag
     4381 gcgctgcgcg gtcgcccgcc cgatccccga gccggcgccg gtgaccaggc cgatcttgcc
     4441 cgccaggcgt ccctcgccgc tcatagcgag atccccccgt cgagggtgat cgtctggccg
     4501 gtcagcatct ccagctccgg gtcgcagagc cgctcgacca gcgccgccgc ctcgccgatc
     4561 tcgaggccgc ggcccatcgg gttggctgcc gcggcggcgg ccagccgttc ctcgtaggtg
     4621 tccgggaaca tcacccgcag ggcggcggtg tcgacgccgc cgggagcgat cgtgttgacc
     4681 cggatcccac gcggggccag ctctttggcg aggtagcgga cgacgtgctc gccgagcgct
     4741 ttcgcggacc cgagcgcgcc gtattcggcg atcacgaagc gggcgccgcg gctggagagg
     4801 tagaagacgt tggagccacg gccgagcagc gggccggcct cgcgcacgag gtcgacgagg
     4861 gcgaggccgt tgacgacgat cgattcgcgc agctcggtcg ggtcggcgtc gacgatcggg
     4921 ccgcgcaccg ccttcgcggc gcagtggacg agctggtcga gctgctcgag ctgctcgacc
     4981 ccggcctcgc ggactgcgtc gatcaccgcg tgcacgccct ccggggtgcc gacgtcggct
     5041 ttgaccaggt gcgggacgcc gccggccgcc tcgatcccgg cgaccgcttc ctctgccgcc
     5101 tcgtcgttgg cgtggtagtt gacgatcacg tcgttgccgt cgtgggcgaa ccgttcggcg
     5161 atcgcccggc cgaggccgct cgagccgccg gtcacgcaga tggacatctc gctcctcttc
     5221 agttggttgc ggtcaggcgc cttcccaagg cgaagctccc agatgcccgg tgccttccca
     5281 actaacggtg cggttcgccg ttgtggaatc gactaagggt ggacgggcag gcgttcggcg
     5341 ggagcggggg gcgcggatac cgtcgaatac gtgatcgact tcgagttgga ccccgagctg
     5401 ctggagctgc gcgaacgcgt ccgcgagttc atccgcgagg tggcgatccc gaacgagccg
     5461 cgcgacgtcg gcacccacgg gaccgacgag gggctgcggg cggagctgca ggagcaggcg
     5521 cgcgagtggg gcgtgttcgc gccccagatc agcacggagc tgggcgggct cgggctcgac
     5581 caccggggca ccgcggtgat cctcgaggag tccggctaca gcctgctcgg cgcccaggcg
     5641 ctgaactgcg ccgccccgga cgagggcacg attcacatgc tcgacctgat cgccaaccca
     5701 gagcagcgcg agcgctacct ggtgccgctc gccgccggca cggcgcgctc ctgcttctcg
     5761 atgaccgagc cggcgccggg cgcgggctcg gacccctcga tgttgctgac cacggccgag
     5821 aagaccgaca gcggctgggt gatcaacggt cgcaagtggt tcatcaccgg cgccgagggc
     5881 gccgccttca ccgtctgcat ggcgaagacc ggcgagggcg cgacgatgtt cctggtcgac
     5941 gccgacaacc ccggcttcca gatcgaccgc gtgatcgagg cgaccgaccg cgccttcccc
     6001 ggtggccacg gcgaagtcac cttcgagaac tgcgaggtcg gcgaggacgc ggtcctgggc
     6061 gaggtcggcg agggcttccg ctacgcgcag gtgcgcctgg caccggcccg gctgacccac
     6121 tgcgagcgct ggctcgggac cgcgcggcgc gccttcgaca tctcgctcga ctatgtcaac
     6181 gagcgcgaag cgttcggttc gaagctcggg gagctcggga tggtgcagga gaagatcgcc
     6241 cgctcggtga tggacatcga gtcgagccgg gcgatgatct ggcgctgcgc ctgggcgctc
     6301 gaccgtggtg agccggccaa acacgagtcc tcggtggcga aggcgtttgt cgccgaggcg
     6361 gtcaaccggg tcgtcgaccg cgccgtccag gtctgcgggg cgaccgggat ctccggcgac
     6421 accctgctgg cgcggatgat gaacgagctg cggccattcc ggatctacga cggaccgacc
     6481 gagacccacc tctggtcgat cgcccgccgg accctgcgcg gccacgccgc gcggaccgcg
     6541 caggccccct cgccgtgaac gcggcgggag ccagctccgg cgacgtcgtc cacaccccgg
     6601 ccgaagcggc cgccgcgcag ctcgagccgc tggtcgtgct ggagccgctc gaggcgttcc
     6661 tcgacggcgc gggcctcggc agtgggccgc tgagcgcggc gccgatcggc gagggccact
     6721 cgaacgtcac ctacctgatc gagcgcggcg gcgagaagct cgtactgcgg cgaccgccgc
     6781 gcggaccgct gccgccctcg gcgcacgacg tcctgcgcga agcgcgcctg ctgatcgcgc
     6841 tgcgtcccca cgggactccg gtccccgagg tgctggcgac ctgcgatgac ccggaggtga
     6901 tcggagcgcc cttctacctg atgaccttcg tcgacggctg ggtcctggcc ggcgacctgc
     6961 cggcgccgct ggcgggcgcc ggggcgccga cgcggatcgg cgaggagctg gtcgacaacc
     7021 tggtcgagct gcacacgctc ggcccgagcg ccgagggcct ggccgggttc ggccgtcccg
     7081 acggctatct ggagcgccag ctgaagcgct tcggcgggct cctggagacg aacatgaccc
     7141 ggccgctgcc ggacctggag cgagtgaccg agtggctctc ggcaaacctc cccgcctcgc
     7201 ccccaccgac cgtcgttcac ggcgactacc gcctcggcaa cgtgatgtac gcgcccgacg
     7261 agccccgcct ggccgcgatc ctcgactggg agatggcgac gatcggcgac cccctcgccg
     7321 atgtcggcta catgaccgcc acctgggccg ggcgcgacga cccgcgcaac tcggtcgccg
     7381 acctctcccc cgtcacccgc ctgcccggat tccccagccg cgacgacctg gcccgccgct
     7441 acgcagagcg cagcggccgc acgatcgagg cgctcggctg gtaccaggtg ctggcgatct
     7501 ggaaggcggc gatcttcctc gagggcaact acaaacggtt cgtcgccggg acgacggcgg
     7561 atccgtacta cgcgagcctc gagcagggtg tgccggagct cgcacaggcg gcgctggcgc
     7621 gcgcccaggg cgcctgactt cgttttcacc gttactgaaa cggtcaaagc tcacatacat
     7681 atgagttcct agtccccagc cttgacagta agggtgaggg tgagatacac cgcccatca
//
LOCUS       DASRIG010000105         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1616365, whole genome shotgun sequence.
ACCESSION   DASRIG010000105 DASRIG010000000
VERSION     DASRIG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616365"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1407)
                     /gene="miaB"
                     /locus_tag="VFB52_02865"
     CDS             complement(82..1407)
                     /gene="miaB"
                     /locus_tag="VFB52_02865"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZO05301.1"
                     /translation="MTKSFHVTTFGCQMNVHDSERMRGMLASLGYDEAASREEADVIL
                     FNTCSIRETADSRFIAHLGEAKRLKGENPERVVGVGGCWAQSVKDEVFQRFPFVDVAF
                     GPGQIHKLAEFLNSDSLSAQGYFEFEDFSGHLPSRREREFQGWLQISQGCNCKCSYCI
                     VPSTRGREESRDPGELIAEVHGLAADGVREVTLLGQNVNSYGRDLPQESRIGFAELLH
                     RVDAVDGIERIRYTSPHPKDMKEDVIRAHAELPALCEQIHLPLQSGSSAILKRMRRTY
                     DRQRYMDRVALIREHVPDCAITTDIIVGFPGETEADFEQTMEVVEEVAYDGAFTFVFS
                     PRRETEAATFGEQVPHPVKVERMERLVEAVQRRATERAQRFVGRTMDVLVEGTSRTDE
                     TKLRGRTRHNKAVNFDGVAEPGEFVDVAIDSATSQTLSGVSAKPLSRLL"
     gene            complement(1508..2125)
                     /locus_tag="VFB52_02870"
     CDS             complement(1508..2125)
                     /locus_tag="VFB52_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZO05302.1"
                     /translation="MKETVFYIVGIALAISAVIVSFIGLKVEKFPGKLSVVVILVFAA
                     GAIGAATFAVLYSKEHEEHREHELEHANEVFESEEHGGPFEEGEEEHGGSEGGEETGG
                     ESGGGEASGPGGTLEVIANETNIAYEESSLETEAGEVEIDFKNPSAIEHDVKVEKDGE
                     EIGGTDVVTDGEASGTVELEPGEYTFFCSIPGHREAGMEGPLTVK"
     gene            complement(2157..3257)
                     /gene="recA"
                     /locus_tag="VFB52_02875"
     CDS             complement(2157..3257)
                     /gene="recA"
                     /locus_tag="VFB52_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZO05303.1"
                     /translation="MAEKKVNLKDLSAKEAKAKDAALEAAVTHVRREFGEGSLMRLGD
                     KGTVKVDSIPTGSLALDLALGIGGVPRGRIVEIFGPESSGKTTLVSHIIAQAQQLGGV
                     CAFIDTEHAIDPVYARAIGVDTDELLVSQPDYGEQALQIADRLIASGAIDIVAIDSVA
                     ALTPKAELDGQIGDTTVGLQARLMSQAMRKLAGNLNRTKTVCIFTNQIREKVGVQFGS
                     PETQPGGRALKFYASQRLDIRRIETLKEGTEAVGNRVRVKVVKNKVAAPFRQAEFDIE
                     YGQGISREGSLIDFGIEHDMVQKSGSFFSYGDTRLGQGRNNAKQFLADNPEIALELET
                     KVRTALGIDGEAAAEPEAETTAETAVPEAQAA"
     gene            complement(3279..3869)
                     /gene="thpR"
                     /locus_tag="VFB52_02880"
     CDS             complement(3279..3869)
                     /gene="thpR"
                     /locus_tag="VFB52_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZO05304.1"
                     /translation="MEAEREKSKRLRVFVALDLPDDVRAGIAAWGRGSLADPALRAVA
                     EESLHITLAFLGSRDPEEVESIAEVVRACGAPAPEIVLGQPQQRPLRGRAKVYALPVA
                     SEGTKALQAGLVSRLVEANLYEAEERPFWPHVTVARVRSERGKKRRPMAVERPPETVP
                     QELLQAFRAVRLSFYISELQPEGARYVPLAQVELPR"
     gene            complement(3927..>4834)
                     /locus_tag="VFB52_02885"
     CDS             complement(3927..>4834)
                     /locus_tag="VFB52_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZO05305.1"
                     /translation="KQAMVPEGALALDPVGTAPGLVVPAGERVVIVLPGPPRELQPMW
                     PTALETAPVREILARSTALHGYTMRMFGVPESEIAKSLRELEGDDGIAVGDVEITTCL
                     RRGEIEIDIRFRDEQAAIADQVRDGLAARHPKETFSLDGATIDEQVARLLVGRRLGLA
                     ESCSGGLMAARITDRRGSSAYMAGSVVAYSNEAKAELLGVDPALIEAKGAVSPEVAEA
                     MAIGALERFGADVAVSITGIAGPDGGTEEKPVGYVCFNARLADGTVIARDPVIPGDRL
                     DIRERSALVGMHLLRTLLGGSDPAL"
BASE COUNT          738 a         1660 c         1590 g          846 t
ORIGIN      
        1 cgatctcccg caggttttca gcgagcgcaa tcgccacacc ggtcttgccg actccggttg
       61 gaccgaagat cgcgaggatg gtcaaagaag ccgggagagg ggcttcgcgg acaccccgct
      121 caacgtctgt gaagtcgcgg agtcaatcgc tacgtctacg aactcaccgg gctccgcgac
      181 cccgtcgaag ttgaccgcct tgttgtggcg ggtgcggccg cgcagcttgg tctcgtcggt
      241 gcggctggtc ccttcgacca gcacgtccat cgtgcgaccg acgaagcgct gggcgcgttc
      301 ggtggcgcgc cgctggaccg cctcgaccag ccgttccatg cgctcgacct tgaccgggtg
      361 cgggacctgc tcgccgaagg tcgccgcctc ggtctcgcgg cgcggcgaga agacgaacgt
      421 gaaggcgccg tcgtaggcga cctcttcgac cacctccatc gtctgctcga agtcggcctc
      481 ggtctcaccg ggaaagccga cgatgatgtc ggtggtgatc gcgcagtcgg gaacgtgctc
      541 gcggatcagc gccacccggt ccatgtagcg ctggcggtcg taggtccgcc gcatccgttt
      601 gaggatcgcg ctcgagcccg actgcagcgg caggtggatc tgctcgcaga gcgccggcag
      661 ctcggcgtgg gcgcggatca cgtcctcctt catgtccttc gggtgcgggc tggtgtagcg
      721 gatgcgctcg atcccgtcga ccgcgtcgac ccggtgcagc agctcggcga agccgatccg
      781 gctctcctgc ggcagatcgc gcccgtagct gttcacgttc tgccccagca gcgtcacctc
      841 gcggactccg tccgcggcga ggccgtgaac ctcggcgatc agctcgcccg ggtcgcggct
      901 ctcctcgcgc cctctggttg agggcacgat gcagtaggag catttgcagt tgcagccctg
      961 ggagatctgc agccagccct gaaactcgcg ctcgcgtcgg gacggcaggt gtccggagaa
     1021 gtcctcgaac tcgaagtagc cctgggcgct caacgaatcg gagttgagga actcggccag
     1081 cttgtggatc tggccgggcc cgaaggcgac gtcgacgaag gggaagcgtt ggaagacctc
     1141 gtccttgacc gactgcgccc aacagccccc gacgccgacc acccgctcgg ggttttcgcc
     1201 cttcagccgc ttcgcctcgc ccaggtgggc gatgaagcgg ctgtcagccg tctcgcggat
     1261 cgagcaggtg ttgaagagga tcacgtcggc ctcttcgcga ctcgcagcct cgtcatagcc
     1321 gagcgaggcc agcatgcccc gcatccgctc ggagtcatgc acgttcatct gacacccgaa
     1381 ggtggtgacg tggaagctct tggtcacgtc aagagggtac tgacggtcaa ggagccaagc
     1441 aggtcaagga cgcagggcgc gccgcgtgct ctgcacgcaa gcaaccgagg acgcagagat
     1501 gcgccggcta cttgaccgtc agcgggcctt ccatgccggc ctcgcgatgg ccggggatcg
     1561 agcagaagaa ggtgtactcg cccggttcga gctcgaccgt cccggaggct tcgccgtccg
     1621 tgaccacgtc ggtgccgccg atctcttcgc cgtctttttc gactttgacg tcgtgttcga
     1681 tcgccgaggg gtttttgaag tcgatctcga cttcgcccgc ctcggtttcc aggctcgact
     1741 cttcgtaggc gatgttggtt tcgttggcga tcacttcgag cgtgccaccg ggcccggaag
     1801 cttcgccgcc accgctctca ccgccggttt cctcgccacc ttcagagccg ccgtgctcct
     1861 cttcgccttc ttcgaaggga ccgccgtgct cctcggactc gaacacctcg ttggcgtgct
     1921 cgagctcgtg ctcgcggtgt tcctcgtgtt ccttcgagta gaggaccgcg aaggtggcag
     1981 cgccaatcgc gccggcagca aaaacgagaa tcacgacgac actcagtttg ccggggaatt
     2041 tctcgacttt gaggccgatg aaactgacga tgacggccga aatcgccagc gcgattccga
     2101 ctatgtagaa aacggtctcc ttcatgaggc gggcggaatc ctatccgcca gcaggtctag
     2161 gcggcttgcg cctccggaac ggccgtctca gcggtcgtct ccgcctcggg ctcggccgca
     2221 gcctcgccgt cgatcccgag cgccgtccgc accttggttt cgagctccag cgcgatttcc
     2281 gggttgtcgg cgaggaactg tttggcgttg ttacgcccct gaccgagccg ggtgtcgccg
     2341 taggagaaga acgagcctga tttctggacc atgtcgtgct cgatgccgaa gtcgatcagg
     2401 ctgccctcgc gcgagatgcc ctggccgtac tcgatgtcga actcggcctg gcggaacggc
     2461 gcagcgacct tgttcttgac gactttcacc cggacccggt tgccgaccgc ctcggtccct
     2521 tccttgaggg tctcgatgcg gcggatgtcg agccgctgcg aggcgtagaa cttcagcgcc
     2581 cggccacccg gctgggtctc cggcgagccg aactggacgc cgactttttc gcggatctgg
     2641 ttggtgaaga tgcagaccgt tttcgtccgg ttgaggttgc cggcgagttt gcgcatcgcc
     2701 tgcgacatca gccgcgcctg cagaccgacc gtggtgtcgc cgatctggcc atcgagctcg
     2761 gctttcgggg tcagcgccgc gaccgagtcg atcgcaacga tgtcgatggc gccggaggcg
     2821 atcagccgat cggcgatctg cagcgcctgc tcgccgtagt cgggctgcga gaccagcagc
     2881 tcgtcggtgt caaccccgat cgcccgcgcg tagaccgggt cgatcgcgtg ctcggtgtcg
     2941 atgaaggcgc agacgccgcc caactgctgg gcctgggcga tgatgtggct caccagggtc
     3001 gtcttacccg aggactccgg gccgaagatc tcgacgatcc ggccgcgcgg cacgccgccg
     3061 atcccgagcg ccagatcgag cgccagcgag ccggtcggga tcgagtcgac cttgaccgtg
     3121 cctttgtcgc cgagccgcat cagcgatccc tcgccgaact cccgccgcac gtgggtgacc
     3181 gccgcctcga gtgcggcatc cttcgctttg gcctctttcg ccgagaggtc tttcaagttc
     3241 acttttttct ctgccatcta gatcacctca ctgccgccct atcgaggcag ctcgacttgg
     3301 gccagcggga cgtagcgcgc accctctggc tggagttcag aaatgtagaa gctgagccgg
     3361 acggctcgga acgcctgcaa gagctcctgc gggaccgttt ccggcggtcg ctcgacagcc
     3421 atcgggcgac gcttcttgcc ccgctcgctg cggaccctcg caaccgtcac gtgcggccag
     3481 aacggacgct cctcagcctc atagaggttc gcctcgacca atctggacac aaggcctgcc
     3541 tgcagcgctt tcgtgccctc agaagcgacc ggaagcgcgt agaccttggc cctgccgcgc
     3601 aacgggcgct gctgtggctg accgagcacg atctctggcg ccggcgcgcc gcacgcgcgg
     3661 acgacctcgg cgatcgactc gacctcctcg ggatcacgcg agccgaggaa ggcgagggtg
     3721 atgtgcagtg actcctcagc gaccgcgcga agcgccgggt cggcgaggct ccctcttccc
     3781 cacgccgcaa tcccggctcg cacgtcatcg ggcaggtcga gtgccacaaa cacgcgcagc
     3841 cgcttcgatt tctcccgctc agcctccacg cgacccacgc taggcggcga aagcgcacga
     3901 ctcaacgcgc gttcgcgaat tcttcgtcac aacgcggggt cagaaccgcc caggagggtg
     3961 cgtaggaggt gcatgccgac gagggcggag cgttcgcgga tgtcgaggcg gtcgccggga
     4021 atgaccgggt cgcgggcgat cactgtgccg tcggcgagcc gggcgttgaa gcagacgtag
     4081 ccgaccggct tctcctcggt gccgccgtcg ggtccggcga tgccggtgat cgaaacggcg
     4141 acgtcggcgc cgaagcgctc cagggcgccg atcgccatcg cctcggcgac ctcgggcgag
     4201 accgcgccct tggcctcgat cagcgccggg tcgacgccga gcaactcggc cttggcctcg
     4261 ttcgagtagg cgacgacgct gccggccatg taggccgaag aaccacggcg atcggtgatc
     4321 cgggccgcca tcaggccgcc gctacaggac tcggcgaggc cgaggcgccg cccgacgagc
     4381 agccgggcga cctgctcgtc gatcgtcgcg ccatcgaggc tgaaggtctc tttcgggtgg
     4441 cgggcggcaa gcccgtcgcg gacctggtcg gcgatcgccg cctgctcgtc gcggaagcgg
     4501 atgtcgatct cgatctcgcc gcggcgcagg caggtggtga tctcgacgtc accgaccgcg
     4561 attccgtcgt ccccctccag ctcccgcagg cttttcgcga tctccgactc gggcacgccg
     4621 aacatccgca tcgtgtagcc gtgcagcgcc gtcgagcgcg ccaggatctc gcgcaccggc
     4681 gcggtctcca gcgccgtcgg ccacatcggc tgcagctcgc gcggcgggcc cggcagcacg
     4741 atcactaccc gctcgccggc cggcacgacc aggcccggag ccgtccccac ggggtcgagg
     4801 gccagcgcgc cctccggcac catcgcctgc ttgc
//
LOCUS       DASRIG010000106         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1109806, whole genome shotgun sequence.
ACCESSION   DASRIG010000106 DASRIG010000000
VERSION     DASRIG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109806"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VFB52_02890"
     CDS             <1..1375
                     /locus_tag="VFB52_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZO05306.1"
                     /translation="GASGEVTEIYVEAGEKVNKNEILARVDSSSARASLASAEAQLAE
                     AEETLESAEEAEVSTTYDSSETTVLASYDGGGGEETSTVLLATVEGGVQEEVPADTGK
                     EGSGETGSGEEEDAPKEESGGTGGSGGKEPKSEPKPDKEEEAAPTQQEAPATEAAPES
                     SGGSAPSTGGGETSTPSATVSVATAEANLRQAELSVESAEQEVSETTLRAPFSGTVAS
                     ISGAVGETVSGEASSGSSSPSSGSSEASSGGLALGGEGSSGGSGETSSGFIVLTQLDR
                     LKMEVSFSEADIGKVEVGQPATVSISSMEGTELAGRVTKVDVLPSESSSGGVVEYPAT
                     IVLTQRAKGLRAGMSASAEVVVEQVADAVIVPNEAIASGPSKTVTVSEDGREVERTVE
                     TGLVGDETTEVLSGLEPGETLVLPEASVATGGLEGGGEGGLPDFGGGGLPSFGGGAPP
                     SGFPGAP"
     gene            1375..2118
                     /locus_tag="VFB52_02895"
     CDS             1375..2118
                     /locus_tag="VFB52_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05307.1"
                     /translation="MDTRRHPVIRIDGLTRTYLMGQMRVQALRGVSLRIERGEYVAIM
                     GPSGSGKSTLMNTLGCLDAPTSGRYLLDGVDVRTHSEDELSDLRNRKIGFVFQGFNLV
                     TKISALANVELPLAYAGLSRPERRRRALRALARVGMADRVGHSSNELSGGQQQRVAIA
                     RAIVTNPALILADEPTGNLDSRSTADVLGIFAALNRHGRTVVMITHEDEVAAHAQRLI
                     RLADGRVVSDLAVEEDPVSEHEHAVLRSA"
     gene            2118..3317
                     /locus_tag="VFB52_02900"
     CDS             2118..3317
                     /locus_tag="VFB52_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05308.1"
                     /translation="MAVETLRTAWVGLMSHRMRTALTVLGITIGIASVIVLIAVGKGS
                     SNAVQESIDALGSNTLTVMATPGATTLGSASVSVTLTEEDVEALQDSEQAPAIKSASP
                     VLNASGVELVHESSSYEPSEFVGTTPGYAEARNYQVEAGSFFTGEQVSERARVAVIGP
                     EVATELFPGQSAVGQTIQADGKSFEVIGVTKSKGSSGTQSQDDVVLAPISAVEDSLAG
                     YNELSSITVQAVDKKSIAAAEAQITSVLNERHGISEGITVINQGSIIEASSSSEGVFT
                     TLLAWVAAISLLVGGIGVMNIMLVSVTERTREIGIRKAIGARRGDILAQFLAEALLVS
                     ALGGLLGIAVGLIGSQFRISGVQPSVELYSIVLAAGASLGSGLFFGTYPAMRAARLRP
                     IEALRFE"
     gene            3310..3978
                     /locus_tag="VFB52_02905"
     CDS             3310..3978
                     /locus_tag="VFB52_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05309.1"
                     /translation="MSEHDDKREAEDEMTRTIEPENDEIVEDEEWVESESDGWQDDEE
                     ELPPRPRNRLLAPLPIFLIAVLIAAGGFLAGVLIQKGSEDGGTAGGFPGGGDLPALAA
                     AGEGEAAGGFPAGLGGASDAAVTGTVSSVNGKTIYVKDADGNTVAVKAGDGSTITRDA
                     NTGVKKIRPGDTVVVQGSTNGSTVRASSISATEAGVESGLPEFGGAGAATEGGDSVES
                     LFGE"
     gene            4064..4636
                     /locus_tag="VFB52_02910"
     CDS             4064..4636
                     /locus_tag="VFB52_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05310.1"
                     /translation="MLALLAVLALVAAVVVGGCGGGSGGDSTSAEAGADATASSGGET
                     DAGGMPAGGPGGFEIDDETRECLEEQGVELPDPGEGGGPPEGGAPPSGGEPPEGFDGG
                     EDMQKAFEECGVDAPQGGGPSGGPPTDSPEFEESIEDYVACVRENGFDLPDPNLSGEG
                     PVFDESEVDQSDPKFKAASEECQSLIGPTG"
     gene            4777..>5155
                     /locus_tag="VFB52_02915"
     CDS             4777..>5155
                     /locus_tag="VFB52_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO05311.1"
                     /translation="MRPKVLILTGPPGVGKTTVGRILTERSERGVHLEVDHFFFFIRS
                     GLIDPWEPESAAQNEVAMRAAAAAAARYAEAGYATIFDGIVVPRWTLGVIRETIEAAG
                     LPVAYAVLRAPQSECLARFVEREG"
BASE COUNT          906 a         1749 c         1821 g          679 t
ORIGIN      
        1 cggcgcctcc ggggaagtca ccgagatcta cgtcgaagcg ggcgagaagg tcaacaagaa
       61 cgagatcctg gcgcgcgtcg actccagctc cgcccgggcc tcgctggcct ccgccgaagc
      121 ccagttggca gaagccgaag aaacgctcga atctgccgaa gaagccgaag tctccaccac
      181 ctatgacagc agcgagacga ccgtcctcgc gagctacgac ggtggcggtg gggaggaaac
      241 gagcaccgtg ctgctggcga cggtggaagg cggcgtgcag gaggaagtgc cggcggacac
      301 cggaaaagaa ggctccggtg aaaccgggtc gggcgaagaa gaagacgccc ccaaagaaga
      361 atccggcggc acgggcggct cgggcgggaa ggaacccaaa tccgaaccga agccggacaa
      421 ggaagaagaa gccgcgccca cccagcagga agcgcccgcc accgaagcgg cgccggaatc
      481 ctccggcggc tcggccccgt cgacgggcgg cggagaaacc tcgacgccgt ccgccacggt
      541 cagcgtcgcc accgccgaag cgaacctgcg ccaggcggag ctgagcgtcg agagcgctga
      601 acaggaagtc agcgaaacga ccctgcgggc gccgttctcg ggcaccgtcg cctcgatctc
      661 cggcgcggtc ggtgaaaccg tgagcggcga agcgagcagt ggatcctcca gcccgagcag
      721 cggttcgagc gaagcgtcga gcggcggcct cgcgctcggc ggggaagggt cgagcggcgg
      781 ctccggcgaa acctcctcgg gtttcatcgt gctcacccag ctcgaccgcc tgaagatgga
      841 ggtctccttc agcgaggccg acatcggcaa ggtcgaggtc gggcagccgg cgacggtctc
      901 gatcagctcg atggaaggca ccgaactggc gggccgcgtg accaaggtcg acgtgctgcc
      961 gagcgaaagc tcgagcggcg gagtggtcga atacccggcg acgatcgtgc tcacccagcg
     1021 ggcgaagggc ctgcgggcgg ggatgtcggc gagcgccgaa gtcgtggtcg aacaggtcgc
     1081 cgacgcggtc atcgtcccca acgaagcgat cgcctcggga ccgagcaaaa cggtcacggt
     1141 cagcgaagac ggccgcgaag tcgagaggac ggtggagacc ggcctggtcg gcgacgaaac
     1201 gaccgaggtc ctcagcggct tggagccggg cgaaaccctg gtgctgccgg aagcgagcgt
     1261 cgccaccggg ggccttgaag gcggtggcga aggcggcctc ccggacttcg gcggcggcgg
     1321 cttgccgagc ttcggcggcg gtgccccgcc cagtggcttc ccgggagccc cgtgatggac
     1381 acgcgccgcc acccggtcat ccgcatcgac ggcctcaccc gcacctacct gatggggcag
     1441 atgcgggtgc aggcgctgcg cggcgtctcg ctgcggatcg agcgtggcga gtacgtcgcg
     1501 atcatgggcc cctcgggcag cggcaagtcg acgctgatga acacgctcgg ctgcctcgac
     1561 gcgccgacca gcggccgcta cctgctcgac ggcgtcgatg tccgcaccca cagcgaggac
     1621 gagctctccg acctgcgcaa ccgcaagatc ggcttcgtct tccagggctt caacctggtc
     1681 acgaagatca gcgccctggc gaacgtggaa ctgccgctcg cctacgccgg cctgtcccgg
     1741 cccgagcgcc gccgccgcgc cctgcgggcg cttgcccggg tggggatggc cgaccgcgtc
     1801 ggccacagct ccaacgagct ttccggcggc cagcaacagc gggtcgcgat cgcccgcgcg
     1861 atcgtcacca acccggcgct gatcctcgcc gacgagccga ccggcaacct cgactcgcgc
     1921 tcgaccgccg acgttctcgg aatcttcgcg gcgctgaacc ggcacgggcg cacggtcgtg
     1981 atgatcaccc acgaggacga ggtcgccgcc cacgcacagc ggctgatccg cctcgccgac
     2041 gggcgcgtcg tctccgacct cgcggtcgag gaggatcccg tctccgagca cgagcacgcc
     2101 gtgttgagga gcgcctgatg gcggtcgaga cgctgcgcac cgcctgggtc ggcctgatgt
     2161 cgcaccggat gcgcacggcg ctgaccgtgc tcgggatcac gatcgggatc gcctcggtga
     2221 tcgtcctgat cgccgtcggc aagggctcct cgaacgcggt ccaggaaagc atcgacgcgc
     2281 tcggttccaa caccctgacg gtgatggcga cgccgggcgc gaccacgctc gggtccgcca
     2341 gcgtctcggt caccctgacc gaggaggacg tcgaagcgct gcaggactcc gaacaggcgc
     2401 cggcgatcaa gagcgcctcg ccggtcctca acgcgagcgg ggtcgaactg gtccatgaaa
     2461 gctccagcta cgaaccgagc gaattcgtcg gcaccacccc gggctacgcg gaagcgcgca
     2521 actaccaggt cgaagcgggc tcgttcttca ccggcgagca ggtctcagaa cgcgcccgcg
     2581 tggcggtgat cggccccgaa gtcgcgaccg aactcttccc cggccagagc gcggtcggtc
     2641 agacgatcca ggccgacggc aagagcttcg aagtgatcgg ggtgaccaag tcgaagggct
     2701 cgagcgggac ccagagccag gacgacgtcg tgctggcgcc gatcagcgcg gtcgaagact
     2761 cgctcgcggg ctacaacgaa ctcagctcga tcaccgtgca ggcggtcgac aagaagtcga
     2821 tcgcggctgc ggaagcgcag atcacctcgg tgctgaacga gcggcacggg atcagcgaag
     2881 ggatcaccgt gatcaaccag ggctcgatca tcgaagcctc gagctccagc gaaggcgtct
     2941 tcaccaccct gctcgcctgg gtggcggcga tctcgctgct ggtcggcggg atcggggtga
     3001 tgaacatcat gctcgtcagc gtcaccgagc ggacccgcga gatcgggatc cgcaaggcga
     3061 tcggcgcccg ccgcggcgac atcctcgccc agttcctcgc cgaggcgctg ctggtctcgg
     3121 cgctgggcgg tctgctcggg atcgcggtcg ggctgatcgg cagccagttc cggatctccg
     3181 gcgtgcagcc gtccgtcgag ctgtactcga tcgtcctagc cgccggggcc tcgctcggct
     3241 ccggcctctt cttcggcacc tacccggcaa tgcgggcggc gcgcctgcga ccgatcgagg
     3301 cgctgcgctt tgagtgagca cgacgacaaa cgcgaggcgg aggacgagat gacgcgaacg
     3361 atcgagcccg agaacgacga gatcgtcgag gacgaggagt gggtcgagtc cgagtccgac
     3421 ggctggcagg acgacgagga ggagctgccg ccgcggccgc gcaaccgtct gctggcaccg
     3481 ctgccgatct tcctgatcgc ggtgctgatc gccgcgggtg gctttctcgc cggcgtgctg
     3541 atccagaagg gcagcgaaga cggcggcacg gctggcgggt tccccggcgg cggcgatctt
     3601 cccgcccttg ccgccgcagg tgaaggcgaa gcggccggcg gctttccggc cggcctcggt
     3661 ggcgcctcag acgccgcggt taccgggacc gtttccagcg tcaacggcaa gacgatctac
     3721 gtcaaggacg ccgacggcaa caccgtggcg gtcaaggcgg gcgacggctc gacgatcacc
     3781 cgcgacgcga acaccggcgt gaagaagatc cgacccggag acaccgtcgt ggtccagggc
     3841 tccacgaacg gctcgacggt gagggccagc tcgatcagcg cgaccgaagc cggcgtcgaa
     3901 tccggcctgc ccgaattcgg cggcgcgggc gccgccaccg aaggcggcga ctccgtggaa
     3961 tcgctgttcg gcgaatgagc ccggtcccac aacttccgaa cgactccgaa acgagaggaa
     4021 cacgatgaaa gaacagaagg actccagatc cagtcgaagg ccgatgctcg cgctactcgc
     4081 cgtactcgcc ctggttgccg cggtcgtggt cggcggttgc ggtggcggct ccggcggcga
     4141 ctcgaccagc gcggaagccg gcgccgacgc gaccgcgtcc agcggcggcg aaaccgacgc
     4201 gggcggcatg cccgcgggcg gtcccggcgg cttcgaaatc gacgacgaaa cgcgcgaatg
     4261 cctcgaggaa cagggcgtcg aactgcccga tccgggtgaa ggcggcggtc ctcccgaagg
     4321 tggcgccccg ccctccggcg gcgaaccacc ggagggcttc gacggcggcg aagacatgca
     4381 gaaggccttc gaagagtgcg gcgtcgacgc tccccagggc ggcggcccga gcggcggtcc
     4441 cccgaccgac tcacccgaat tcgaagaatc gatcgaagac tacgtcgcct gtgtgcgcga
     4501 aaacggcttc gacctgcccg acccgaacct gagcggcgaa ggtccggtct tcgacgaatc
     4561 cgaggtcgat cagagcgatc ccaagttcaa agccgcgagc gaagagtgcc agtccctcat
     4621 tggcccgacg gggtgatctc tgtcgggacc ctcgaggacg tcgtcctcgt cctcgtcgtc
     4681 ctgctcttcg tcaccgccgt cgccgtggac ctccgtctgc ggagacggcg ggacggccgc
     4741 tgaacaggtt ggcgcacgac gatagatttg gcgcggatgc gcccgaaggt tctgatcctc
     4801 accggcccgc ccggggtcgg gaagacgacc gtcggcagga tcctcaccga acgctccgag
     4861 cggggcgtcc acctcgaagt cgatcacttc ttctttttca tccgcagcgg cttgatcgat
     4921 ccctgggagc ccgagtcggc ggcgcagaac gaggtggcga tgagggccgc ggccgcggcc
     4981 gccgcacgct acgcggaggc cggctacgcg acgatcttcg acggcatcgt ggtcccgcgc
     5041 tggacgctgg gcgtgatccg ggagacgatc gaggccgccg ggctgccggt cgcctacgcc
     5101 gtgctgcgcg cgcctcagtc ggagtgcctc gcccgtttcg tcgagcgcga aggca
//
LOCUS       DASRIG010000107         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3709029, whole genome shotgun sequence.
ACCESSION   DASRIG010000107 DASRIG010000000
VERSION     DASRIG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709029"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /gene="clpB"
                     /locus_tag="VFB52_02920"
     CDS             complement(<1..2355)
                     /gene="clpB"
                     /locus_tag="VFB52_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZO05312.1"
                     /translation="MQADRFTVKSQEAIQAALELARSHRNTEAAPPHLLVALLDQPEG
                     LVVPILQRVGADTATIAARASEAVAALPKLGGEVDEPRMSSTLAETLRKAEQQMARLG
                     DEYISTDHLLLALTESSLGEFLPDRDSIDRAIGEVRPQKVTSPNPESTAQALEKFGRD
                     LTAEARAGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVD
                     GDVPESLRDRRVISLDIGSLLAGAKYRGEFEERLKAVLSEVQEAEGQIVLFLDELHTI
                     VGAGAAEGAVDAANLLKPMLARGELRCVGATTLDEYRKHIEKDAALERRFQPVLVGEP
                     DINSTIAILRGLKPRYEEHHGVRIADAAIVAAATLSERYIADRFLPDKAIDLIDEAAS
                     RLKIEIDSVPTEIDEVERRIQQLEIEREALKKESDDASKARREALEAELANLGEEATE
                     LRTRWQNEKEPIEAIKEARGQLEEARVEVEQAEREANLERAAELKFGRIPELEEKVKE
                     QAARLEELHAGGEVLLGDEVTEEDVAEVVGKWTGIPVSRLMEGEVEKLIHMEERLHDR
                     VIGQDEAIEAVSNALRRSRAGLSDPNRPIGSFLFLGPTGVGKTELAKALAEFMFDSEQ
                     AIVRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYAVVLLDEIEKAHPD
                     VFNTLLQIMDDGRLTDGQGRTVSFTNTVLIMTSNVGSDRIVGEQVDEAIREQIEEVLA
                     ATFKPEFLNRIDDTVIFHRLSKADIGRIVELQVAQLVGRVRERGI"
     gene            complement(2355..2705)
                     /locus_tag="VFB52_02925"
     CDS             complement(2355..2705)
                     /locus_tag="VFB52_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO05313.1"
                     /translation="MPAYRRRVKASFDEEQGVFMISVAAELAEMHPQTLRMYEQRGLI
                     APKRSPKNTRLYSQRDVERLRRIQEMTAEGLNLAGVETVLALEERVAKLVTELDRARK
                     RIEQLEETTKGKLH"
     gene            complement(2705..>3445)
                     /locus_tag="VFB52_02930"
     CDS             complement(2705..>3445)
                     /locus_tag="VFB52_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZO05314.1"
                     /translation="EIQLSFDQAVNGDQISVTVPKSETCQTCHGSGAKPGTSPVTCPR
                     CEGTGVDAQGQGLFSISRPCPQCGGAGEIIEDPCPTCGGSGLTQQTKRYKVNVPAGVK
                     DGTRIRLAGKGEAGPRGGPHGDLYVTTRVAASPVFKRLDDGNLEVTVPISIPEALNGG
                     TIEVPTLEGTKKIKVAAGTKHGAIQRLRGEGPPKPKGKGRGDIRYRLEIEVPKELTDK
                     QREAAEKLAESFNGDDPRAELLRKAGAH"
BASE COUNT          404 a         1235 c         1167 g          639 t
ORIGIN      
        1 gatcccgcgc tcgcgcacgc ggccgaccag ctgcgccacc tgcagctcga cgatccggcc
       61 gatgtcggcc ttcgagagcc gatggaagat caccgtgtcg tcgatccggt tgaggaactc
      121 gggcttgaag gtcgccgcca gcacctcttc gatctgctcg cggatcgcct cgtcgacctg
      181 ctcaccgacg atccggtcgg agccgacgtt cgaggtcatg atcagcaccg tgttggtgaa
      241 gctgaccgtg cggccctggc cgtcggtgag gcggccgtcg tccatgatct gcagcagggt
      301 gttgaagacg tccgggtgcg ccttctcgat ctcgtcgagc agcacgaccg cgtagggccg
      361 gcggcgcacc gcctcggtca gctggccgcc ctcctcgtag ccgacgtagc cgggcggtgc
      421 cccgatcagg cgggcgaccg tgtgcttctc catgtactcg gacatgtcga ggcggacgat
      481 cgcctgctcg gagtcgaaca tgaactcggc cagcgccttc gccagctcgg tcttgccgac
      541 cccggtcggg ccgaggaaga ggaaggagcc gatcggccgg ttcgggtccg agagcccggc
      601 gcgcgagcgc cgcagcgcgt tggagaccgc ctcgatcgcc tcgtcctggc cgatcacccg
      661 gtcgtgcagg cgctcctcca tgtggatcag cttctcgacc tcgccctcca tcagccggct
      721 gaccgggatc ccggtccatt tgccgaccac ctcggcgacg tcctcctcgg tgacctcgtc
      781 gccgagcagc acctcgccgc cggcgtgcag ctcttcgagc cgcgccgcct gctctttgac
      841 cttctcctcg agctcgggga tgcggccgaa tttgagttcg gcggcgcgct ccaggttggc
      901 ctcgcgttcg gcctgctcga cctcgacccg ggcctcctcg agctgtccgc gcgcctcttt
      961 gatcgcctcg atcggctctt tctcgttctg ccagcgggtg cgcagctcgg ttgcctcctc
     1021 gcccaggttg gcgagctcgg cctcgagcgc ctcccgccgc gcctttgagg cgtcgtcgct
     1081 ctccttcttc agcgcctcgc gctcgatctc gagctgctgg atccgccgct cgacctcgtc
     1141 gatctcggtc ggcaccgagt cgatctcgat cttcagccgc gaggccgcct cgtcgatcag
     1201 gtcgatcgcc ttgtcgggca ggaagcggtc ggcgatgtag cgctccgaga gcgtcgccgc
     1261 ggcgacgatc gccgcgtcgg cgatgcgcac gccgtggtgc tcctcgtagc gcggtttcag
     1321 cccgcgcagg atcgcgatcg tgctgttgat gtcgggctcg ccgaccagca ccggctggaa
     1381 gcgccgctcc agcgctgcgt ctttctcgat gtgcttgcgg tactcgtcga gcgtggttgc
     1441 gccgacgcag cgcagctcgc cgcgcgccag catcggcttc aagaggttgg ccgcgtcgac
     1501 cgcgccttcg gcggcgccgg caccgacgat cgtgtggagc tcgtcgagga agaggacgat
     1561 ctggccctcc gcctcctgca cctcggagag caccgctttc agccgctcct cgaactcgcc
     1621 gcggtattta gcgccggcca ggagcgagcc gatgtcgagc gagatcaccc gccggtcgcg
     1681 caggctctcg ggcacgtcgc cgtcgacgat ccgccgcgcc agcccctcga cgatcgcggt
     1741 cttgccgacc ccgggctcgc cgatcagcac cgggttgttc ttggtccggc gcgagagcac
     1801 ctggaccacc cggcggatct cctcgtcgcg gccgatcacc gggtcgagct tgcccgcgcg
     1861 cgcctcggcg gtcaggtcgc ggccgaattt ctccagcgcc tgcgccgtcg actcgggatt
     1921 gggggaggtc accttctgcg ggcgcacctc gccgatcgcc ctgtcgatcg agtcgcggtc
     1981 ggggaggaac tcgcccagcg aggactcggt cagcgccagc agcaggtggt cggtggagat
     2041 gtactcgtcg ccgagccgcg ccatctgctg ctcggccttg cgcagggtct cggccagggt
     2101 cgaggacatc cgtggctcgt cgacctcgcc gccgagtttc ggcagcgccg cgaccgcctc
     2161 gctggcgcgc gccgcgatcg tcgcggtgtc ggcgccgacc cgctgcagga tcgggacgac
     2221 caggccttcg ggctggtcga gcagggcgac gagcaggtgg gggggagccg cttcggtgtt
     2281 gcggtggctg cgcgccagct ccagcgccgc ctggatggct tcctgcgatt tgaccgtgaa
     2341 cctgtctgcc tgcatcagtg aagttttcct ttcgtcgtct cctccagttg ttcgatccgt
     2401 ttgcgcgccc ggtccagttc ggtgacgagc ttcgcgaccc gctcttccag cgccagcacc
     2461 gtctccacgc ccgcgaggtt cagaccctcg gcggtcattt cctggatccg gcgcagccgt
     2521 tcgacgtcgc gctgggagta gagccgggtg ttcttcggcg agcgcttcgg cgcgatcagc
     2581 ccgcgctgct cgtacatccg cagcgtctgc gggtgcatct cggccagttc ggcggcgacc
     2641 gagatcatga acacgccctg ctcctcgtcg aagctcgctt tcacgcggcg gcggtaggcg
     2701 ggcatcagtg cgcccccgcc ttgcgcagca gctcggcccg cgggtcgtcg ccgttgaacg
     2761 actcggctag cttctcggcc gcctcgcgct gcttgtcggt cagctctttc gggacctcga
     2821 tctcgagccg gtagcggatg tcgccgcgac ccttgccctt cggcttcggc ggaccctcac
     2881 cccgcagccg ctggatcgcc ccgtgcttgg tgccggcggc gaccttgatc ttcttcgttc
     2941 cctccagcgt cggcacctcg atcgtgccgc cgttcagcgc ctccgggatc gagatcggca
     3001 ccgtcacctc gaggttgccg tcgtcgagcc gtttgaacac gggcgaggcg gcgacccggg
     3061 tggtcacgta gaggtcgccg tggggaccgc cgcgcggccc ggcctcgcct ttgccggcga
     3121 gccggatccg ggtgccgtcc ttgaccccgg cggggacgtt gactttgtag cgcttggtct
     3181 gctgggtgag gccggagccg ccgcaggtcg ggcaggggtc ttcgatgatc tcgccggcgc
     3241 cgccgcactg cgggcacggc cggctgatcg agaacaggcc ctggccctgg gcgtcgaccc
     3301 cggtcccctc gcagcgcggg caggtgaccg ggctggtgcc cggtttggcg ccgctgccgt
     3361 gacaggtctg gcaggtctcc gacttcggca cggtgacgct gatctggtcg ccgttgaccg
     3421 cctggtcgaa gctcagctgg atttc
//
LOCUS       DASRIG010000108         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3709151, whole genome shotgun sequence.
ACCESSION   DASRIG010000108 DASRIG010000000
VERSION     DASRIG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709151"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFB52_02935"
     CDS             complement(<1..294)
                     /locus_tag="VFB52_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05315.1"
                     /translation="MTWVVIALLCLAVAVLLAALLREHRRLRPLERLIEDMEKVDLSR
                     PGMALPASIDGVGETEEVERLELAFLRMMRRLEAERRRAGSAALRAQEEERARV"
     gene            443..1303
                     /locus_tag="VFB52_02940"
     CDS             443..1303
                     /locus_tag="VFB52_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZO05316.1"
                     /translation="MKTTKQSSFGATIRTTILLASLSGLLVLIGALIGGPSTAAIFLG
                     IALLINLGSYFFSDKLALKMSGAQPIEESEAPRLYQIVRELTTRAGLPMPRLYMIPQD
                     QPNAFATGRNPQHSAVAVTRGITKLLSEDELRGVLAHELAHIQHRDILIQSVASAIGA
                     AITYLAYMFMWFGSEDESPLSIVASLAMVLLAPIAATIIQLSISRQREYAADAGGAEI
                     CGNPESLASALLRLEQGAEAIPMKVNQAAEPLYIVKPFKGGGIAGLFSTHPPIEERVK
                     RLRQMRPSLG"
     gene            1395..2213
                     /locus_tag="VFB52_02945"
     CDS             1395..2213
                     /locus_tag="VFB52_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05317.1"
                     /translation="MRLGLRTSALAIAATAMLSLALPAGASATKVGFTAIAEPASECF
                     PPKGNKYASTSCQVFLGNSLEIIAVASRDDGSVRLAPQPFTLLRVNPGNGTTVVTSFT
                     LFDENEADDQPIITPRRNTDYKLRFNGNEVIPAETSAPLVVEVGAELSIPEGSSTGSG
                     KGVGIPVGVTVPDPSLRGKLELRRCHRTKAFSAASCARPSSYTVLSRRAVNRSDQLVL
                     SIAVDPRSYERYEIAFRPRSRQFATTRQAFNLSRAGSGPITYRQTVRASPFGNR"
     gene            2304..3473
                     /gene="moeB"
                     /locus_tag="VFB52_02950"
     CDS             2304..3473
                     /gene="moeB"
                     /locus_tag="VFB52_02950"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZO05318.1"
                     /translation="MATDQLQQIEEIAPFEAQAEIEGGDVVLIDTREPHEYKEAHLEG
                     GKLVPPGLLADEIGDAAPDKSARTILYCRSGNRSAIAAAQLQAMGYENVASMAGGILL
                     WQEQGLPVVAASGMTAEQRERYSRHTLLPEVGVDGQVKLLNSKVLLLGAGGLGAPTAL
                     YLAAAGVGTIGLVDDDVVDASNLQRQVIHNTERIGMAKTASARIFIEGLNPDVNVVEH
                     NGRLDASNILPILEQYDIVVDGADNFPTRYLLNDASVRLRIPVVSASILGFDGQISTF
                     VPYEGPCYRCLYPTPPPAELAPSCSANGVLGVMAGTMGTLQANEVIKLILGAGDPLVG
                     RLLLYDALGTTFTELKVRRDPDCPICSDNAPEIPESEMGQFPDYDLFCAGAGGDR"
     gene            3505..3780
                     /locus_tag="VFB52_02955"
     CDS             3505..3780
                     /locus_tag="VFB52_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZO05319.1"
                     /translation="MATVKIPPVLRAKTGGASEVEAGGSNVGEVLRTLTSDHPDTSAQ
                     LFGEDGELNRYVNVYLNDEDVRVLDGLETAVKDGDTVVILPAMAGGR"
     gene            3770..4270
                     /locus_tag="VFB52_02960"
     CDS             3770..4270
                     /locus_tag="VFB52_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO05320.1"
                     /translation="MGASPQPSGKLLATERGRDLVLARTFDAGIEQVWAELTEPERTA
                     RWFGPWSGSGEPGGTISVRMAFEQGEPEIEMVVDECERPRRLALHSEDEHGSWHLEVE
                     LSEVGGTTLQLTHHLDEKAEPGMVGPGWEYYLDMFVAAHAGQALPSFDDYFPSQQAYY
                     VGLVPE"
     gene            4364..4642
                     /locus_tag="VFB52_02965"
     CDS             4364..4642
                     /locus_tag="VFB52_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05321.1"
                     /translation="MAGEGQDLRLGPAAPEGAILAAWVEDLGAKAAMLADDPETYFTT
                     AHFDNYAIVLARLERIDVEELREVVVEAWLARAPKRLAKEYADSALED"
     gene            complement(4625..5389)
                     /locus_tag="VFB52_02970"
     CDS             complement(4625..5389)
                     /locus_tag="VFB52_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZO05322.1"
                     /translation="MAEPMLSLQGIHKRYGDLEVLKGVDLDVAKGEVVCILGPSGSGK
                     STLLRCVNLLEPPEDGEIFLEGKDICKGPGSGTGEQSWNLDFVRQRVGMVFQQFNLFP
                     HKSAMANVTMAPEKVLGRSKSEAREKAKALLERVGLGDKLDQYPERLSGGQQQRVAIA
                     RALAMDPHVMLFDEVTSALDPELVKEVLDVLRELADEGMTMLVVTHELGFAREVGDKV
                     VFMDGGVIVEQGPPAEVLDNPQQERTKKFLGLVLER"
     gene            complement(5393..>5732)
                     /locus_tag="VFB52_02975"
     CDS             complement(5393..>5732)
                     /locus_tag="VFB52_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO05323.1"
                     /translation="IPRGQIEAARSLGMSHSQAMRNVVVPQAVTKIPAPMLNELISLL
                     KDTTLVGVIALGEALLIGREVQAETFNTSAYVLGCAMFLVICLPAARLVDYLIKRNQG
                     KVARGSHIAV"
BASE COUNT          924 a         1990 c         1929 g          889 t
ORIGIN      
        1 gacgcgggcg cgctcctcct cctgggcgcg cagcgccgcc gagccggccc gccgccgctc
       61 cgcttccagg cgccgcatca tccgcaggaa ggcgagctcc agccgctcca cctcctcggt
      121 ctcgccgacc ccgtcgatcg aggcgggcag cgccattccc ggccggctca ggtcgacctt
      181 ctccatgtcc tcgatcagcc gctccagggg ccgcagccgc cggtgctcgc gcagcagcgc
      241 cgccagcagc acggcgacgg cgaggcagag cagggcgatc acgacccagg tcacgggccc
      301 agcttaagag ggatcgccag ggcgcctggg tgctggaccg gcgcgaatgg gggttgcccc
      361 ctatggccaa atcccctgct cagcggaata ctcgttcgac tacaacgccc tttccgggct
      421 tatccaggag caactacacg cgatgaagac caccaagcag tcgagtttcg gcgccacgat
      481 tcgcacgacg atcctgctgg cctcgctttc cggcctgctc gtcctgatcg gcgccctgat
      541 cggcggcccc agcacggccg cgatcttcct cggcatcgcg ctgctgatca acctcggctc
      601 ctacttcttc agcgacaaac tggcgctgaa aatgagcggg gcgcagccga tcgaggagtc
      661 cgaagcgccg cggctctacc agatcgttcg cgaactgacg acccgcgccg ggctgccgat
      721 gccgcggctc tacatgatcc cccaggacca gccgaacgcc ttcgccacgg gtcgtaaccc
      781 gcagcactcg gcggtcgcgg tcacccgcgg gatcaccaaa ctcctctccg aggacgagct
      841 gcgcggcgtg ctcgcacacg agctcgcgca catccagcac cgcgacatcc tgatccagtc
      901 ggtcgcctcg gcgatcggcg cggcgatcac ctatctcgcc tacatgttca tgtggttcgg
      961 cagcgaggac gagtcgccgc tcagcatcgt cgcctcgctg gcgatggtcc tgctcgcgcc
     1021 gattgcggca acgatcatcc agctctcgat ctcgcgccag cgcgagtacg cggccgacgc
     1081 cggcggtgcc gaaatctgcg gcaaccccga gtcgctggcc agcgcgctgc tgcggctcga
     1141 gcagggcgcc gaagcgatcc cgatgaaagt caaccaggcg gccgagccgc tctacatcgt
     1201 caagcccttc aagggcggtg ggatcgccgg tctcttctcg acgcaccccc cgatcgaaga
     1261 gcgcgtcaag cgccttcgcc agatgcgtcc cagcctcggc tagccaggag agggaaagca
     1321 gcaccgagcg ggccgccttc gggcggcccg ctttgcgtta ggcgggggac cggcgcgggt
     1381 agggtgcgcg cacgatgcgg ctcggcctcc gaacgtctgc cctggcgatt gccgcgaccg
     1441 cgatgctgtc cctggcgctg ccggcgggcg cgagcgcgac gaaggtcggg ttcacggcca
     1501 tcgccgagcc ggcgagcgag tgcttcccgc ccaagggcaa caagtatgcg agcacctcgt
     1561 gccaggtgtt cctcggcaac tcgctcgaaa tcatcgcggt ggcgagccgc gacgacggct
     1621 cggtgcggct ggcgccgcag ccgttcacgc tgctgcgcgt gaacccgggc aacggcacca
     1681 ccgtggtcac cagcttcacg ttgttcgacg aaaacgaagc cgatgaccag ccgatcatca
     1741 ccccgcggcg caacaccgac tacaagctgc gcttcaacgg caatgaagtc atcccggcgg
     1801 agacctcggc gccgctggtg gtcgaagtcg gggcggagct gagcatcccc gaagggtcca
     1861 gcaccggctc gggcaaaggg gtcgggatcc cggtcggggt gacggtccct gacccgtcgc
     1921 tgcgcggcaa gctcgagctg cgccgctgcc accggaccaa ggcgttcagc gccgcgagct
     1981 gcgcccgccc gagcagctac accgtcctct cgcgccgcgc ggtcaaccgc agcgaccagc
     2041 tcgtcctctc catcgcggtc gacccgcgca gctacgagcg ctacgagatc gccttccgcc
     2101 cgcgctcccg gcagttcgcg acgacccgcc aggccttcaa cctgagccgc gccggcagcg
     2161 ggccaatcac ctatcggcag acggtccgcg cttcgccgtt cggcaaccgc tgaagctcgc
     2221 ttgcaatgcg ggctgaagcg gcattgacaa gtcgctatac tttttgaagt aaaagccgat
     2281 ccgaattagg ggatttcaac ttcatggcca ctgaccagct tcagcagatc gaggagatcg
     2341 caccgttcga ggcgcaggct gagatcgagg gcggcgacgt cgtcctgatc gatacgcgcg
     2401 agccgcacga gtacaaggag gctcacctcg agggtgggaa gctggtgccg ccggggctgc
     2461 tggccgacga gatcggggac gcggcgccgg acaaatcggc gcgcacgatc ctctactgcc
     2521 gctccggcaa tcgctcggcg atcgccgcgg cgcagctgca ggcgatgggc tatgagaacg
     2581 tcgcctcgat ggcgggcggg attctgctct ggcaggagca ggggctgccg gttgtcgccg
     2641 cctccgggat gaccgcggag cagcgcgagc gctactcgcg ccacacgctg ctgcccgagg
     2701 tcggggtcga cgggcaggtc aagctgctga actcgaaagt gctgctgctc ggcgctggcg
     2761 ggctcggcgc gccgaccgcg ctctatctcg ccgcggccgg ggtcggcacg atcgggctgg
     2821 tcgacgacga cgtcgtcgac gcttccaacc tgcagcgcca ggtgatccac aacaccgaac
     2881 ggatcgggat ggcgaagacc gcttcggcgc ggatcttcat cgaaggcctc aaccccgacg
     2941 tgaacgtggt cgagcacaac ggccgcctcg acgcctcgaa catcctgccg atcctcgagc
     3001 agtacgacat cgtcgtcgac ggcgccgaca acttccccac ccgttacctg ctgaacgacg
     3061 cttcggtgcg gctgcgcatc ccggtcgtct ccgcttcgat cctcggcttc gacgggcaga
     3121 tctcgacctt cgtgccctac gagggcccct gctaccgctg cctctacccg accccgccgc
     3181 cggcggagct ggcgccgagc tgctcggcga acggcgtgct cggcgtgatg gccgggacga
     3241 tgggcacctt gcaggccaac gaggtgatca agctgatcct cggcgccggc gatccgctgg
     3301 tcggtcgcct gctcctctac gacgccctcg gcaccacctt caccgagctc aaggtccgcc
     3361 gcgaccccga ctgcccgatc tgcagcgaca acgcgcccga gatccccgag tcggagatgg
     3421 gtcagttccc ggactacgac ctcttctgcg ccggcgccgg cggggaccgc taaacagctc
     3481 ccgctgcatt aggatcaccg ctcgatggcg accgtgaaga tcccaccagt gctgcgcgcc
     3541 aaaaccggcg gtgcctccga ggtcgaggcc ggggggagca acgtcggcga ggtcctgcgc
     3601 acgctgacca gcgatcaccc cgacacttcg gcgcagctgt tcggcgagga cggggagctg
     3661 aaccgctacg tcaacgtcta cctcaacgac gaggacgtgc gggtgctcga cggcttggag
     3721 accgccgtca aagacggcga caccgtcgtg atcctgccgg cgatggccgg tgggcgctag
     3781 cccgcagccg agcggaaagc tcctggccac cgagcgtggc cgcgatctcg tcctcgcgcg
     3841 caccttcgac gccgggatcg agcaggtctg ggctgagctg accgagcccg agcggaccgc
     3901 gcgctggttc gggccgtggt ccgggagcgg cgagccgggc gggacgatct ctgtgcggat
     3961 ggccttcgag cagggcgagc cggagatcga gatggtggtc gacgagtgcg agcgaccgcg
     4021 tcgcctcgcg ctccacagcg aggatgagca cggcagctgg catctggagg tggagctgag
     4081 cgaggttggt ggcaccacgc ttcagctgac ccaccacctc gacgagaagg ccgagccggg
     4141 gatggttggg ccgggctggg agtactacct cgacatgttc gtcgccgccc acgccgggca
     4201 ggcgctgccg agcttcgacg actacttccc ctcgcaacag gcctactacg tgggcctcgt
     4261 cccggagtag ggtcgggggc cgggtggcga gctgggcgga cgtcgagcgg ctcgcattgg
     4321 ccctgccgga gacggccgag ggcagctcac gcgatctgcg ggtgtggcag gtgaagggca
     4381 agaccttcgt ctgggaccgg ccgctccgga gggggcgatc ctcgctgcct gggtcgagga
     4441 cctcggggcg aaggcggcga tgctcgccga cgatcccgag acctacttca cgaccgccca
     4501 cttcgacaac tacgcgatcg tgctcgcgcg gctggagcgg atcgacgtcg aggagttgcg
     4561 cgaggtggtg gtggaagcgt ggttggcgcg ggcgccgaaa cggctggcga aggaatacgc
     4621 cgactcagcg ctcgaggact agcccgagga acttcttcgt gcgctcctgc tggggattgt
     4681 cgagcacctc ggcgggcggg ccctgctcga cgatcacgcc gccgtccatg aacacgactt
     4741 tgtcgccgac ctcacgggcg aagccgagct cgtgggtgac gaccagcatc gtcatccctt
     4801 cgtcggcgag ctcgcgcagc acgtcgagca cctctttgac cagctcgggg tcgagcgcgc
     4861 tggtcacctc gtcgaacagc atcacgtgcg ggtccatcgc cagggcgcgg gcgatcgcca
     4921 cgcgctgctg ctgaccgccg gagagacgtt cggggtactg atccagtttg tcgccgaggc
     4981 cgacgcgctc gagcagggcc ttggctttct cccgcgcctc ggacttggaa cggccgagga
     5041 ctttttccgg cgccatggtc acgttcgcca tggcgctctt gtgggggaag aggttgaact
     5101 gttggaagac catcccgacc cgctgacgca cgaagtcgag gttccagctc tgctcgccgg
     5161 tcccggagcc ggggcccttg cagatgtcct tgccctcgag gaagatctca ccgtcctccg
     5221 gtggctcgag caggttgacg cagcgcagca gggtgctctt gcccgagcca ctcgggccga
     5281 ggatgcagac gacttcgccc ttggcgacgt cgaggtcgac gcctttgagc acctccaggt
     5341 cgccgtagcg cttgtggatc ccctgcaggc tgagcatcgg ttcggccatc ggtcagaccg
     5401 cgatgtggga gccgcgggcg accttgccct ggttgcgctt gatcaggtag tcgaccaggc
     5461 gggccgcggg caggcagatc accaggaaca tggcgcagcc gagtacgtac gccgatgtgt
     5521 tgaaggtttc cgcctggact tcgcggccga tcagcagggc ctcaccgagc gcgatcacgc
     5581 cgaccagggt cgtgtccttc aacagcgaga tcaattcgtt cagcatcggc gccgggatct
     5641 tcgtcacggc ctggggcacg acgacgtttc tcatcgcctg cgagtggctc atcccgagcg
     5701 agcgcgccgc ctcgatctgg ccgcgcggga tc
//
LOCUS       DASRIG010000109         6418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_314468, whole genome shotgun sequence.
ACCESSION   DASRIG010000109 DASRIG010000000
VERSION     DASRIG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314468"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFB52_02980"
     CDS             <1..665
                     /locus_tag="VFB52_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05324.1"
                     /translation="LAAEGAEVWVGDIDKAGAEQVAGEVNGHPLDLDVTEIESARAAL
                     AETGPLDILINNAGTDEFGFFTYTSPEQWQRVIAINLVGVLNCTHAALPGMQDAKYGR
                     IVSISSEAGRVGSKGSAVYSAAKGGIIAFMKAIARENARYGITANSIAPGPIETPLLM
                     GAKEFGEIGEKVIETMKAATQIGRLGQPEEVAAAVAFLASDDATYVTGETLGVSGGLG
                     MV"
     gene            711..1640
                     /locus_tag="VFB52_02985"
     CDS             711..1640
                     /locus_tag="VFB52_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZO05325.1"
                     /translation="MGGLKQQAARERSAATWSLARRQHGVVARRQLTELGFSRHAIAH
                     RVEIGRLHRVGPAVYAVGLPDLTRRGRWMAAVLSCGAGALLTHNSAAALWGFGKEWGR
                     GIEVATRGSSPRSRPGVRVHRRPSLRPGSVAERDRIPVTTPVQTVIDLATQYGRSSLE
                     RMISEADQLGLFTPPSLRAALPEHAGEPGVAFLRATLDRRTFRLTRSELERAFLPIAA
                     AVRLPVPLTKQMVNGFEVDFYWPDLRFVVETDGLTYHRTPAQQAEDRRRDQAHTAAGL
                     TNLRFTHEQVRYEPDYVRRILAETARRLGVCAS"
     gene            complement(2122..2316)
                     /locus_tag="VFB52_02990"
     CDS             complement(2122..2316)
                     /locus_tag="VFB52_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05326.1"
                     /translation="MGSSPTSGITPGRGQAQRRQRREAPGGADQVGADHVADEVPLEH
                     GDAEADREREAGAGQRGRPL"
     gene            complement(2292..2375)
                     /locus_tag="VFB52_02995"
     tRNA            complement(2292..2375)
                     /locus_tag="VFB52_02995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2339..2341),aa:Leu,seq:caa)
     gene            complement(2424..2948)
                     /locus_tag="VFB52_03000"
     CDS             complement(2424..2948)
                     /locus_tag="VFB52_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05327.1"
                     /translation="MAVGEVRPGYELWRPNREKAGSVTTKFVIVLLMLATAALAAVIT
                     IGGWSLLKGGGGMGIVCAIYAILYAFFAYLVARWSRGILPLAAALSMILAIFCAVGAN
                     SWFERDKAGFDEALLPTALLGTLVVILGLLQIVLIAAAMYGFNQEWHVEEERPIGSGE
                     DYGAGAAGSAPQPA"
     gene            3022..3105
                     /locus_tag="VFB52_03005"
     tRNA            3022..3105
                     /locus_tag="VFB52_03005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3056..3058,aa:Leu,seq:tag)
     gene            3125..4204
                     /locus_tag="VFB52_03010"
     CDS             3125..4204
                     /locus_tag="VFB52_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZO05328.1"
                     /translation="MRRVIRHLKTGEDGVALSSAVIVCLVLVSGVIIGLLLFNAMSGD
                     EKVQSGPLEQEQAAAREARLEEELRELNAAKEKQAAKTDVPAATAKSRDEYEGIASGG
                     GGGSGGNALSGDAEASFSSLEAEAGASVGLAIAPFGGSVQTLGSTEGRHAWSSIKVPI
                     LATLMRQRQLSSSDQANAEAALTASDNNAAAALFSAIEGTNSALESTLNETASEPTVV
                     ATAPPPSGAASTYGQTLWSPAASAEFYRALACGQVLDSGSTSYVLGLMGNVISEQQWG
                     LASASFPGASYVGYKAGWGPEGSASGPYLVRQSGIVEDGNGNGFAVTMVAQASSGSFE
                     GGVAALNTVAGWLSQHLGKGAYGAC"
     gene            4262..4780
                     /locus_tag="VFB52_03015"
     CDS             4262..4780
                     /locus_tag="VFB52_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05329.1"
                     /translation="MGVRMKARFGSRSVWLLAVLPVVLMLSLGLAACGGGGASDEEIS
                     EAEKAGIAKERQKQRLDNLEAKLKQGLKDLKKGQGSKGSAPNPPSTAPAPAPAPSSSE
                     GNCGGSLSVNSVTTCEFAENVRFAYEAEVGSGSGSIEAFSPAKGIYYDMYCSGGSPVE
                     CTGGVGAWVSFP"
     gene            4792..5283
                     /locus_tag="VFB52_03020"
     CDS             4792..5283
                     /locus_tag="VFB52_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05330.1"
                     /translation="MGGPRWLAIALVVLVVGGLAAWGIVEATSSDGDGGAGLVESAQE
                     ERVQQRIERLERMLIAIDRADQQPTARQKARRAAARQAARQKQRATRRAQKAAAGKAA
                     AGGGGNCDPSYEGACLDPNAADYDCSDGSGDGPEYTGTVTVVGDDHYGLDSNNDGIGC
                     EEG"
     gene            5330..>6418
                     /locus_tag="VFB52_03025"
     CDS             5330..>6418
                     /locus_tag="VFB52_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05331.1"
                     /translation="MRSKGRVTKVVLLLAALVALGVVISGCAFIKAGSLAVSQPGGVG
                     TVRVHFALCNQPADEDELQTCAPNESKEETTQYLLGIAVPPGSVPPETVTAVAVGGGA
                     PIVFHRNAEVTAQIGSLSGIEEGETKVPVWPPAGTEGVGYLSDPYVEETGTTRELTVD
                     ADFGLPVPADGGAFGGPFRTALAFGLREVSPAQPANRPVQCVGPASPPSESLAFCIPT
                     GFEAQAGTSDLRIKAPKTTTAFVGGKAPLKFGLSFASTAGALPTFAMKATTSLKGGKA
                     SVANPAFVPGNVDPTTHRSPAANSTANVSIPKKAKPGTYEVVLTATTPQGGTVTQVAK
                     LKVTKPKLKLGATKLDKAKGTATLEVKVP"
BASE COUNT         1054 a         2179 c         2236 g          949 t
ORIGIN      
        1 gtttggcggc cgaaggggcc gaggtctggg ttggcgacat cgacaaggcc ggggccgagc
       61 aggtcgcggg cgaggtcaac ggccacccgc tcgacctcga cgtgaccgag atcgagtcgg
      121 cgcgcgcggc cctggcggag accggcccgc tcgacatcct catcaacaac gccggcaccg
      181 acgagttcgg cttcttcacc tacacctcgc ccgagcagtg gcagcgggtg atcgcgatca
      241 acctggtcgg ggtgctcaac tgcacgcacg cggcgctgcc ggggatgcag gacgcgaagt
      301 acggccgcat cgtcagcatc tcctcggagg cgggccgggt cggctccaag ggctcggccg
      361 tctactcggc cgcgaagggc gggatcatcg ccttcatgaa ggcgatcgcg cgcgagaacg
      421 cgcgctacgg gatcaccgcc aactcgatcg cccccgggcc gatcgagacg ccgctgctga
      481 tgggcgccaa ggagttcggc gagatcggcg agaaagtgat cgagacgatg aaagcggcga
      541 cccagatcgg ccgcctcggc cagcccgagg aggtcgccgc cgcggtcgcc ttcctcgcca
      601 gcgacgacgc cacctacgtg accggcgaga ccctcggcgt aagcggcggc ctaggcatgg
      661 tctgagcccc gacggggaga gtctcgggtg cttgaagcat taggcctgcc atgggcggcc
      721 tcaagcagca agcggcaaga gagcgaagtg cggcgacgtg gagcttggcg cgcaggcagc
      781 atggggtggt ggcacggagg cagctgaccg agctgggatt cagccggcat gcaatcgctc
      841 atcgggtgga gatcggccgg ctgcaccgcg tcgggcccgc cgtctacgcc gtcggtctgc
      901 cggacctcac ccggcggggc cgctggatgg cggctgttct ctcatgcggc gcgggcgcgc
      961 ttctcaccca caacagcgcc gcggcgcttt ggggcttcgg caaggagtgg gggagaggca
     1021 tcgaagtcgc gacccgcggc tcctcgcccc gcagccgtcc cggcgttcgt gtccaccggc
     1081 gcccgtcgct gcgtccagga tcggtcgccg aacgggaccg cataccggtc accacgccgg
     1141 tccagaccgt catcgacctc gcgacccaat acggccgcag ctcgctggag cggatgatca
     1201 gtgaggcaga ccagctcggc ctcttcaccc cacccagcct gcgcgccgcg ctccccgaac
     1261 acgccggcga accgggcgtc gccttcctcc gtgcgaccct cgatcgccgt acgttccgcc
     1321 tcacgcgctc cgagctcgag cgggccttcc tgccgatcgc ggccgcggtt cgtctcccgg
     1381 tcccgctgac caaacagatg gtcaacggct tcgaggtcga cttctactgg ccggacctgc
     1441 gctttgtcgt cgagaccgac ggcctcacct atcaccgcac gccggcccaa caggcggagg
     1501 atcgacgccg cgatcaggct cacaccgcgg ccggtctcac caacctccgc ttcacccacg
     1561 agcaggttcg ctacgaaccg gattacgtgc gccggatcct cgccgagacg gcacgccgcc
     1621 tgggtgtttg cgcatcctga gggtttctta aggatccgct agtaccgttc gcggatgtcc
     1681 gaccgtgtga aagccccgct cgcggcctgc gccgcctgtg cgctgggcgt ggtcgcattg
     1741 ggcgcgctgg tctacggatt cgggccggtg gagcgggtcg acggcaagct cttcctccat
     1801 cttgccgccg acccgttctc acgcacgcac gaagtggcgg aactggtcgc gcggctggcc
     1861 gacccgctgc cgctggtgtt gatgctggcc gcggtctgcc tgctggcgct gagttggggc
     1921 ggccggcgcg aggcggtggc ggcggtcgcc gtcgtcgccg gggccaacat caccacgcag
     1981 atcctcaagg gggtgctcgc gcacccgcgc taccagccct acacgggcct gaaccagccc
     2041 tggtcggatg cctatcccag cggccacacg accgcggccg cctcgatcgg gatcgcgctg
     2101 gcgctggccg cgccgccgcg gctaaagcgg ccggccgcgc tggccggcgc cggcttcgcg
     2161 ctcgcggtcg gcctctgcgt cgccgtgctc cagtggcact tcgtcagcga cgtggtcggc
     2221 gcctacctgg tcagcgccgc ctggggcttc gcggcgctgg cggcgctgcg cctgaccgcg
     2281 cccaggggtg atgccggagg tgggactcga acccacaagc ccgcaagggc agtcgatttt
     2341 gagtcgaccg cgtatgccaa ttccgccact ccggcgcggt cctgagcccg gagaatagtc
     2401 gcggacagcc agatggggcg gcgctaggcc ggctgagggg cgctgcctgc agcgccggcg
     2461 ccgtagtcct cgccggagcc gatcgggcgc tcctcctcga cgtgccactc ctggttgaac
     2521 ccgtacatgg ccgccgcgat caggacgatc tgcagcagtc cgaggatcac caccagggtg
     2581 ccgagcaggg cggtcggtag caaggcctcg tcgaagcccg ccttgtcgcg ttcgaaccag
     2641 ctgttggcgc cgaccgcgca gaagatcgcg aggatcatcg agagcgccgc cgctagcggc
     2701 aggatcccgc ggctccagcg ggcgaccagg taggcgaaga aggcgtagag gatcgcgtag
     2761 atcgcgcaga cgattcccat cccgccgccg cccttgagca gggaccagcc gccgatcgtg
     2821 atcacggccg cgagcgccgc cgtggccagc atcaaaagga cgatcacgaa cttggtcgtg
     2881 accgagccgg ccttctcccg attgggacgc cagagctcgt accccggcct cacctctcct
     2941 accgccatgc gtcgatcata gaatccatct gcgacgctgc ggcattgacc gcgcgttaag
     3001 cccagctacg ggggttgcca cgcgggcgtg gcggaattgg aatacgcgcc aggtttaggt
     3061 cctggtgggc ttcggcccgt gggggttcga gtccctccgc ccgcactgac agggtttgcg
     3121 tttgttgaga cgagtaatca ggcatctgaa gacgggggaa gatggcgttg cgctcagcag
     3181 cgcggtgatc gtctgcctgg ttctggtcag cggcgtgatc atcggcctgc tcctcttcaa
     3241 cgcgatgtcc ggggacgaga aggtgcagag cgggccgctt gaacaggagc aggcggcggc
     3301 tcgggaagcg cggctggagg aagaactgcg ggaactgaac gcggccaagg aaaagcaggc
     3361 ggcgaagacc gacgtgccgg cggcgaccgc aaaatcgcgc gacgaatacg aaggcatcgc
     3421 ctccggcggg ggcggtggca gcggcggcaa cgcgctctcg ggtgacgcgg aagcctcgtt
     3481 ctcctcgctg gaagccgaag ctggggcgag cgtggggttg gcgatcgcgc ccttcggggg
     3541 cagcgtgcag acgctcggct cgaccgaagg ccggcacgcc tggagcagca tcaaggtgcc
     3601 gatcctggcg acgctgatgc gccagcgcca gctcagctcg agcgaccagg cgaacgccga
     3661 agcggcgctg accgcgtccg acaacaacgc cgccgcggcg ctcttctccg cgatcgaagg
     3721 gaccaactcg gcactcgaat cgacgctgaa cgaaaccgcg agcgaaccga ccgtggtggc
     3781 gaccgcgccg ccgccctcgg gcgccgcctc gacctacggg cagacgctct ggagcccggc
     3841 cgcctcggcc gagttctacc gcgcgctcgc ctgcggccag gtgcttgact ccgggtcgac
     3901 cagctacgtg ctcggtctga tgggcaacgt gatctccgaa cagcagtggg gcctggccag
     3961 cgccagcttc ccgggcgcct cctacgtcgg ctacaaggcg ggctggggcc cggagggcag
     4021 cgccagcggc ccgtacctgg tgcggcagtc ggggatcgtc gaggacggca atggcaacgg
     4081 cttcgccgtg accatggtcg cccaggcttc ttctgggtcc ttcgaaggcg gcgtcgcggc
     4141 gctcaacacg gtcgccggct ggctcagcca acacctcggc aagggcgcct acggggcctg
     4201 ctagagcgat ttgacttagt ctttccggtg ctcgtaagtt cgattactgg ggagacgtca
     4261 ggtgggggta cgcatgaagg caaggtttgg ttctcgttcg gtatggctgc tggctgtgct
     4321 gccggtggtg ttgatgctct ctttgggcct ggcggcctgc ggcggcggag gggcgagcga
     4381 tgaggaaatc tccgaagccg aaaaagccgg gatcgcgaag gagcgtcaga aacagcgcct
     4441 cgacaacctc gaagcgaaat tgaaacaggg gttgaaagac ctgaagaagg gtcaggggag
     4501 caagggttcc gcgccgaacc cgccgtccac cgcgccggct ccggcacccg ctccgtcgag
     4561 cagcgaaggc aactgcggcg gcagcctctc ggtcaactcg gtgacgacct gcgaattcgc
     4621 cgaaaacgtg cgcttcgcct acgaagcgga agtcggctcg ggcagcgggt cgatcgaagc
     4681 cttcagcccg gcgaaaggca tctactacga catgtactgc agcggtggct cgccggtcga
     4741 atgcaccggt ggtgtcggtg catgggtctc cttcccctga gggcctgagc cgtgggaggg
     4801 ccgcgttggc tcgcgatcgc gctcgtcgtc ctcgtcgtcg gcgggctcgc cgcctgggga
     4861 attgttgaag cgacctcctc tgacggcgac ggcggtgccg gtctcgtcga gagcgcccag
     4921 gaagaacggg ttcagcagcg gatcgaacgt ctggaacgga tgctgattgc gatcgaccgt
     4981 gccgatcagc aaccgacggc gcgccagaag gcgcgacggg cggcggcgcg gcaggcggcc
     5041 cgccagaaac aacgggcgac ccgccgcgcg cagaaagccg ccgctggcaa ggctgccgcg
     5101 ggcggtggtg gcaactgcga tcccagctac gagggcgcct gtctggaccc gaacgcggct
     5161 gactacgact gcagcgacgg ctccggcgac ggccccgagt acaccggcac ggtgaccgtg
     5221 gtcggcgatg accactacgg cctcgattcc aacaacgacg ggatcggttg tgaggaaggc
     5281 tgagcgccgg gttagactgc gcggtccaaa gctgagggga gagagtcaga tgaggtcgaa
     5341 gggccgtgtc accaaggttg tcctgctgct cgcggcgctg gttgcgcttg gggtggtgat
     5401 cagcggctgc gccttcatca aggccggctc gctcgcggtc agccagccag gtggcgtggg
     5461 cacggtgcgg gtccacttcg cgctctgcaa ccagcccgcg gacgaagacg agctccaaac
     5521 ttgcgcgccg aacgagtcga aagaagaaac gactcagtac ctgctcggga ttgcggtccc
     5581 gcccggatcg gtcccgcccg agaccgtgac cgcggttgcg gtcggcggcg gcgctccgat
     5641 cgtcttccac cgcaatgccg aagtcacggc gcagatcggc tcgctcagcg gcatcgaaga
     5701 aggcgaaacg aaagtcccgg tctggccacc ggcggggacc gaaggcgtcg gctatctctc
     5761 cgacccctac gtcgaggaaa ccgggaccac ccgcgaactc accgtcgacg ccgacttcgg
     5821 gctgcccgtg cccgccgatg gcggcgcctt cggcggtccc ttccgcaccg cgctcgcctt
     5881 cggcctccgc gaggtcagtc ccgcccagcc cgcgaaccgg ccggtccagt gcgtcggccc
     5941 cgcatccccg ccgtcggaaa gcttggcgtt ctgtataccc actggcttcg aagcccaggc
     6001 cggcacctcg gacctgcgga ttaaggcgcc gaagacgacc accgctttcg tcggcggcaa
     6061 ggcgccgctc aagttcggcc tctccttcgc cagcaccgcg ggggctctgc cgacctttgc
     6121 gatgaaggcg acgacctcgc tgaagggcgg caaggcttcg gtcgccaacc ccgccttcgt
     6181 gcccggcaac gtcgacccga ccacccaccg ctcgcccgcg gcgaactcga cggcgaacgt
     6241 ctcgatcccg aagaaagcca agccaggcac ctacgaagtt gtgctcaccg cgaccacgcc
     6301 gcaggggggc accgtgaccc aggtcgcgaa gctgaaggtg acgaagccga agctgaaact
     6361 gggcgccacc aagctcgaca aagccaaagg caccgcgacc ctcgaggtca aggtcccg
//
LOCUS       DASRIG010000110         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1110610, whole genome shotgun sequence.
ACCESSION   DASRIG010000110 DASRIG010000000
VERSION     DASRIG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110610"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..455)
                     /gene="rplX"
                     /locus_tag="VFB52_03030"
     CDS             complement(129..455)
                     /gene="rplX"
                     /locus_tag="VFB52_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZO05332.1"
                     /translation="MRIKTDDQVVVTAGKDAGKSGRVVRTEPKRRRVFVEGLNMVKRA
                     ERPRSQGDLKNAQAGGIIEKEGPIDVSNVMLLDPKDNKPTRIGVRLDAGGKRVRYSKR
                     TGEAVD"
     gene            complement(464..835)
                     /gene="rplN"
                     /locus_tag="VFB52_03035"
     CDS             complement(464..835)
                     /gene="rplN"
                     /locus_tag="VFB52_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZO05333.1"
                     /translation="MIQQESRLKVADNSGARELLCIRVKGGSHRRYASVGDVITATVK
                     SASPQGGVRKGEVVQAVVVRTRKPLSRPDGTYISFDENAAVLIDPASNNPRGTRIFGP
                     VARELRDRNFMKIISLAPEVL"
     gene            complement(841..1074)
                     /gene="rpsQ"
                     /locus_tag="VFB52_03040"
     CDS             complement(841..1074)
                     /gene="rpsQ"
                     /locus_tag="VFB52_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZO05334.1"
                     /translation="MRQGIVVSSKGDKSITVRIDIARRHRTYEKIVRSSAKLHAHDER
                     NEAGEGDVVRVIETRPLSKTKRWRLVDVVEKAR"
     gene            complement(1479..1694)
                     /gene="rpmC"
                     /locus_tag="VFB52_03045"
     CDS             complement(1479..1694)
                     /gene="rpmC"
                     /locus_tag="VFB52_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZO05335.1"
                     /translation="MKASEVHNLKDDELVAKLIDAKQEAFNLRFRHATGELENTSRLG
                     QAKRDIARLLTVARARGIDVERETRKA"
     gene            complement(1694..2113)
                     /gene="rplP"
                     /locus_tag="VFB52_03050"
     CDS             complement(1694..2113)
                     /gene="rplP"
                     /locus_tag="VFB52_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZO05336.1"
                     /translation="MLMPKRVKHRKQFRGRMRGNTKGGSSVAFGEYGIKATERGWITN
                     RQIEAARIAMTRKIKRGGKVWINVFPDKPYTKKPAETRMGSGKGNPEGWVAVVKPGKV
                     MFELAGVPEELAREALRLAGHKLPVKTKFVKREGAEG"
     gene            complement(2113..>2624)
                     /gene="rpsC"
                     /locus_tag="VFB52_03055"
     CDS             complement(2113..>2624)
                     /gene="rpsC"
                     /locus_tag="VFB52_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZO05337.1"
                     /translation="ELDAKLVAQSIAEQLQNRVAFRRAMKRALTSAMRSGAKGVKVQV
                     SGRLGGAEMARTEQYSDGRVPLHTLRADIDYGFFEARTTTGRIGVKCWINKGEVMPEG
                     FRSDLASTDEPPQQDRGRGGRDRGGRGPGGDRGPGGGRGPGGDRGGRGGGGRGPGGGG
                     RGGQRGGGR"
BASE COUNT          310 a          915 c          903 g          496 t
ORIGIN      
        1 cggcgcctcc atcggcgtgg tgtaaccgaa tttgcgcgtc agctcgggca ggacctgctg
       61 ctcgtatttc tcgcgcaggc gtggcggcgg cgctgcctgc gaggtcggtg acacggtgcc
      121 cgcctccatc agtcgaccgc ctccccggtg cgcttcgagt agcgcacgcg cttgccaccg
      181 gcatcgaggc gcacgccgat gcgggtcggt ttgttgtctt tgggatcgag cagcatcacg
      241 ttggagacgt cgatcggacc ctccttttcg atgatgccgc cggcctgggc gtttttcagg
      301 tcgccctgcg agcgcggccg ttcggcgcgc ttgaccatgt tcagcccctc gacgaagacg
      361 cggcgccgtt tcggctccgt gcgcacgacg cggccgctct taccggcgtc cttgccggcg
      421 gtgacgacga cctggtcgtc ggtcttgatc cgcatcgact tcatcagagg acctcgggcg
      481 cgagcgagat gattttcatg aagttgcgat cgcgcagttc gcgcgccacc gggccgaaga
      541 tgcgggtccc gcgcgggttg ttgctggccg ggtcgatcag cacggcggcg ttctcgtcga
      601 aggagatgta ggtgccgtcg gggcggctga gcggcttgcg cgtgcgcacg acgaccgcct
      661 gcacgacctc gcccttgcgg acgccgccct gcgggctggc cgatttgacc gtcgcggtga
      721 tcacgtcgcc gacgctggcg taacggcggt gcgaaccgcc cttgacgcgg atgcagagca
      781 gctcacgggc gccggagttg tcggccacct tgagtcggga ctcttgctga atcatctgct
      841 ctacctcgcc ttctcgacca cgtcgaccag gcgccaacgc ttggtcttgg acagcggacg
      901 ggtctcgatc acgcggacca cgtcgccctc accggcctcg ttgcgctcgt catgcgcgtg
      961 cagcttcgcg gagctgcgaa cgatcttctc gtaggtgcgg tgacgacgag cgatgtcgat
     1021 gcggacggtg atcgatttgt cgcctttgct ggaaacgacg atgccctggc gcatcttggc
     1081 ggcgttggcg tcgcgctcgg ccggcggcgt gccgatgccc gagccgtcgc gcttggcttt
     1141 ctcggcagcc cgttctttgc gccgttcggc ggccttgcgg gcacgccggc cagagcgctc
     1201 ggcggcgagc tcgtcggcgc tgcgctgcgg gcgggtctcg cggggcaggc gcgagcgctg
     1261 caggcggcgg cgctccttcc agggaaggtc gtcctcggga ttcggctcgg cgggctcctc
     1321 ggcggcgggg gcggcagcct cagcggccgg cgcctcctcg gccggagcct cctccgcggc
     1381 aggggcctcc tcgaccgcat cctcgacggc cggggtctcc tcggcagcag cctccggagt
     1441 ctcctcggtc gtctcgtctt ttttcgtctc gtcgtcgctc atgctttgcg ggtctctctc
     1501 tcaacgtcga tgccgcgggc gcgcgccacg gtcagcagcc gggcgatgtc gcgcttggcc
     1561 tgtcccaggc gggaagtgtt ctcgagctcg ccggtggcgt ggcggaagcg gaggttgaag
     1621 gcctcctgct tggcgtcgat cagtttcgcc accagctcgt cgtctttcag attgtgcacc
     1681 tcggaagctt tcatcagccc tcggcaccct ctcgtttgac gaatttcgtt tttaccggaa
     1741 gcttgtggcc ggcgaggcgc agcgcttcgc gcgccagctc ctcgggcacg cccgcgagct
     1801 cgaacatcac cttgccgggc ttgaccacgg cgacccagcc ctccgggttg cccttgccgg
     1861 agcccatccg ggtctcggcc ggctttttcg tgtacggctt gtcggggaag acgttgatcc
     1921 agaccttccc gccacgtttg atcttgcggg tcatcgcgat acgagcggcc tcgatctggc
     1981 ggttggtgat ccagccgcgc tcggtcgcct tgatcccgta ctcgccgaag gcgacggagc
     2041 tgccgccctt ggtgttgccg cgcatgcggc cgcggaactg tttgcggtgc ttgacccgtt
     2101 tcggcatcaa catcagcgac cacctccacg ctggccgccg cggccgccgc cgccggggcc
     2161 gcgtccaccg ccgccacgac cgcctcggtc gccgccgggc ccacgaccgc cgccggggcc
     2221 gcgatcgcca ccgggcccac gcccaccgcg gtcgcgaccg ccgcggccgc ggtcctgctg
     2281 cggcggctcg tcggtcgaag cgagatcgga acggaagccc tcgggcatga cctcgccctt
     2341 gttgatccag catttgacgc cgatccggcc ggtcgtcgtg cgcgcctcga agaagccgta
     2401 gtcgatgtcg gcgcgcagcg tgtgcagcgg cacgcgaccg tcggaatact gttcggtccg
     2461 cgccatctcg gcaccgccga ggcggccgga gacctggacc ttcactccct tggcgccgga
     2521 gcgcatcgcg gaggtgaggg cgcgcttcat cgcgcggcgg aacgccaccc ggttctggag
     2581 ctgctcggcg atcgactggg cgaccagctt cgcgtccagc tcgg
//
LOCUS       DASRIG010000111         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2392566, whole genome shotgun sequence.
ACCESSION   DASRIG010000111 DASRIG010000000
VERSION     DASRIG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392566"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VFB52_03060"
     CDS             <1..1391
                     /locus_tag="VFB52_03060"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZO05338.1"
                     /translation="VFVRHRTYDFEMQKNRPWGDAVVTGHGTIDGRTVFVFSQDFTVF
                     GGSLGEVMAEKMCKVMDLAAQVGAPVIGINDSGGARIQEGVVSLGAYGDVFVRNVQCS
                     GVIPQISLIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPEVIKTVTGEEVEFEELG
                     GAMSHNSKSGVAHFASEDEESCLEDVRYLMSFLPSNNLEAPPRFEPSDDPEREDEELD
                     TLVPDDPSKPYDMRDVIARIVDDEEFFEVHEHFAKNIVCGFSRLDGYPVGIVGNQPAF
                     LAGVLDIEASEKAARFVRTCDCYNVPILTFTDVPGFLPGTDQEWNGIIRHGAKLLYAF
                     TEATVPKLTVVTRKAYGGAYDVMNSKHMLADFNVAWPTAEVAVMGPEGAVNIIYRKDI
                     ANSPTPDERRQKLIDDYKAHFANPYSAAERGYIDDVIEPRQTRPKLIKALRMLQSKRV
                     AQPKRKHGNIPL"
     gene            1766..2431
                     /locus_tag="VFB52_03065"
     CDS             1766..2431
                     /locus_tag="VFB52_03065"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZO05339.1"
                     /translation="MAEQRATAGGGQPRPSSRNKRAEILTVATEYFGHRGYEDTKWAD
                     VATKVGVGSTALYHYFESKLHCLYVIIADGVEASRADFDRITAEAEFVEGLHEVLRAG
                     FVLTELQVQSRRVMVAEQGRVGERRRSPREEEARQVARARTRDYEVVWATHLARGMAQ
                     GHLPEADPQLLSRAVIGLYNSIWHWYRPGGVLDLDQVADFYVPRHLTLIGLDPSQNGA
                     SAS"
     gene            complement(2444..>2623)
                     /locus_tag="VFB52_03070"
     CDS             complement(2444..>2623)
                     /locus_tag="VFB52_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05340.1"
                     /translation="TSAGTPGEKAFGKYLAAANSWGACIDEEGCEAATVEPVLQKQWK
                     GAARFLDEAQAGLAA"
BASE COUNT          450 a          897 c          866 g          410 t
ORIGIN      
        1 acgtcttcgt ccgccaccgc acctacgact tcgagatgca gaagaaccgg ccctggggcg
       61 acgccgtcgt caccggccac gggacgatcg acggacgcac ggtcttcgtc ttctcccagg
      121 acttcaccgt cttcggcggc agcctcggcg aggtgatggc cgagaagatg tgcaaggtga
      181 tggacctcgc cgcccaggtc ggcgcgccgg tgatcgggat caacgactcc ggcggcgccc
      241 gcatccagga gggcgtggtc agcctcggcg cttacggcga cgtgttcgtc cgcaacgtcc
      301 agtgctcggg cgtgatcccg cagatcagcc tgatcatggg cccctgcgcc ggcggcgccg
      361 tctactcgcc ggcgatgacc gacttcatct tcatggtcaa ggagacctcg cacatgttca
      421 tcaccggccc ggaagtgatc aaaacggtga ccggcgagga agtcgagttc gaggagctcg
      481 gcggggcgat gagccacaac tcgaagtcgg gcgtcgccca cttcgcctcc gaggacgagg
      541 agagctgcct cgaggacgtg cgctacctga tgagcttcct gccctcgaac aacctcgagg
      601 cgccgccgcg cttcgagccc tccgacgacc cggagcgcga agacgaggag ctcgacacgc
      661 tcgtccccga cgacccttcg aagccctacg acatgcgcga cgtgatcgcc cgcatcgtcg
      721 acgacgaaga gttcttcgag gtccacgaac acttcgccaa aaacatcgtc tgcgggttct
      781 cgcggctcga cggctatccg gtcgggatcg tcggcaacca gcccgccttc ctcgccggcg
      841 tgctcgacat cgaggcctcc gagaaggcgg cccgcttcgt gcgcacctgc gattgctaca
      901 acgtcccgat cctcaccttc accgacgtgc ccggcttcct gcccggcacc gaccaggagt
      961 ggaacgggat catccgccac ggcgccaagc tgctctacgc gttcaccgag gcgaccgtgc
     1021 cgaagctcac cgtggtcacc cgcaaggcct acggcggcgc ctacgacgtg atgaactcga
     1081 agcacatgct ggccgacttc aacgtcgcct ggccgaccgc ggaggtggcg gtgatggggc
     1141 cggagggcgc cgtcaacatc atctaccgca aagacatcgc caactcgccg accccggacg
     1201 agcgccgcca gaaactgatc gacgactaca aagcccactt cgccaacccg tattcggcgg
     1261 cggagcgcgg ctacatcgac gacgtgatcg agccccggca gacgcggccg aagctgatca
     1321 aagcgctgcg catgctgcag tccaagcgcg tcgcccagcc gaagcgcaag cacggcaaca
     1381 tcccgctctg aggccgtctc ctgcgtgggc cattgagccc gactggcgag tttgcttcaa
     1441 tcgccagacc agagaaagga ggtggtccta attttgagtc acatgcttaa ggggactctg
     1501 gaggtgcagg gccgctaggc gccgcactgg ttcgggcgtc cggcggaatc caccccgggc
     1561 accggcgagc gtcagggagt cgggctttgt cccactcgac gcgaattccc gcgctcgctc
     1621 ccaagcacca gacgagggcc gcctccgggc ggccctcgtt atttcttttt tgagcccaag
     1681 ttcggcgctc ctaattcgcg ggtactgtgg atagagtcac ggttcagctc cagcggggga
     1741 aacgaaggag tgaggagcga tacgagtggc agagcagaga gcgacggccg gcgggggcca
     1801 gccccggcct tcctcgcgta acaagcgggc ggagatcctc acggtggcga ccgagtactt
     1861 cggccaccgc ggttacgagg acaccaaatg ggcggacgtg gcgaccaagg tcggggtcgg
     1921 ctcgaccgcg ctgtaccact acttcgagtc gaagcttcac tgtctgtacg tgatcattgc
     1981 cgacggggtc gaggcctcgc gcgcggattt cgacaggatc accgccgagg ccgagttcgt
     2041 cgagggcctg cacgaggtgc tgcgcgccgg cttcgtgctg accgagctgc aggtgcagag
     2101 ccgccgcgtg atggtcgccg agcaaggccg ggtcggtgag cgccgccgct cgccgcggga
     2161 ggaggaggcg cggcaggtgg cgcgcgcccg cacccgcgac tacgaggtgg tctgggcgac
     2221 gcacctggcg cgcgggatgg cgcagggaca cctgcccgag gccgatccgc agctgctcag
     2281 ccgcgccgtg atcggcctct acaacagcat ctggcactgg taccgccccg gcggcgtgct
     2341 cgacctcgac caggtcgccg acttctacgt gccccgccac ctcaccctga tcgggctcga
     2401 cccctctcag aacggcgcca gcgccagcta gctcggggtt gcctcaggcg gcgaggccgg
     2461 cctgggcttc gtcgaggaag cgggcggcgc ccttccactg cttctgcagg accggttcga
     2521 cggtggccgc ttcgcagcct tcttcgtcga tgcaggcgcc ccaggagttg gccgccgcga
     2581 ggtatttgcc gaaggccttc tcgccgggag tgccggcgct ggt
//
LOCUS       DASRIG010000112         7571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_578524, whole genome shotgun sequence.
ACCESSION   DASRIG010000112 DASRIG010000000
VERSION     DASRIG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578524"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..914
                     /gene="sufC"
                     /locus_tag="VFB52_03075"
     CDS             153..914
                     /gene="sufC"
                     /locus_tag="VFB52_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZO05341.1"
                     /translation="MEIRNLHAKVEEKEILKGLDLDVEKGKVHALMGPNGSGKSTLAN
                     VIMGHPAYEVTEGTISFKGEDITEADPDERSQAGLFMAFQYPVAIPGVSVSKYLRMII
                     NAKREAHGEEPIKIKDFAREAQEAMALANIPKDFSSRYLNDGFSGGEKKRMELLQLAL
                     LRPEIAVLDETDSGLDIDALRTVADGVNQFAGPDMGVLIITHYQRILHMVKPDFVHVM
                     FEGRIVKEGGPELVGVLEEKGYGWIAEEVGAGASA"
     gene            937..2202
                     /locus_tag="VFB52_03080"
     CDS             937..2202
                     /locus_tag="VFB52_03080"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZO05342.1"
                     /translation="MSTESVSPVAAPFPIEKVRAAFPALEREIGGKPVAYLDSGASSQ
                     RVLASIQAVDRYERRHHSNVHRGSHTLSAEATEAYEGARATVADHIGAGDRREVVFVR
                     NATEAINLVARAWGDANVGAGDRIVLTEMEHHSNIVPWQQLAERVGAEIDWAPIDEEG
                     LLDMEAFAALLERGPKLVAVTHVSNVLGTENPLTEIARLSHDAGALVLADGAQSAPKL
                     PLDMAALGVDFYAITGHKLYAPTGIGALWTRLDLLREMPPFLGGGSMIRKVTREGTTY
                     ADVPARFEAGTPAITQAVGMAAALRWLDALGMETVRNHERQIADYALARVTEVPGLKV
                     FGPPRGPERLGPVSFEIEGIHAHDVSEILDRHGVAVRAGHHCAQILMDRLGVAATARA
                     SFGVYTTPEEIDRLVDGLEDARRVFGLDA"
     gene            2256..3530
                     /locus_tag="VFB52_03085"
     CDS             2256..3530
                     /locus_tag="VFB52_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HZO05343.1"
                     /translation="MLVLLRTIAVSALLSLAVLASAASAAPRLIALGAGPNVNVLFSF
                     DADNPGATTAVPVTGLGSSSLVGIDRRPADGLLYGLGVDGNAATLFRLEPTTGTASSV
                     GSVTITSLTGASAYGVDFNPVSDRLRVVNDLASGVGTNVNNFRIRPAANLLAGIDSDL
                     DFSGAPGSGSEVAIAHDRGVGASATTLFGVTAIGDMLVRQGGVDGTPSPNGGAISAIG
                     PLGVDITANAGLDIDPTSGEAFAVFTVAGESSLYRVSLATGAATLVGKVGTGTVSFGG
                     LTIDPPVAGPPPPAEPQLESLSLRPRAFATANIGGAILSKVRIGTTVSYSLSAAATTA
                     FTVERKSTGRRVGKACKKLTRGNRDRKPCPLFKPLKAGFTHAGAAGANAFRFTGRIGG
                     NALRPGAYRLVARAGSSSARTTFTVVPRGRTR"
     gene            complement(3527..5092)
                     /locus_tag="VFB52_03090"
     CDS             complement(3527..5092)
                     /locus_tag="VFB52_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05344.1"
                     /translation="MLAVLVALVCAAPAAAAPPANDDFADAETLTGEDPSAFGNVGEA
                     TTEPGEPVHGPGLTTTVWYSWQAPVSRWVTVLRCGGPVNAVEAVYTGSAVDALTLVSR
                     TSVGAGGGCGYAPEWQASFRAVAGTTYRIAVAAEGGANNFQLSFDESQPRFNDTLANS
                     DWLGGTDDLAEGHNVGATTEPREVVPAGSGGATVWYSWTAPSDGVAELSTCGSSFDAR
                     VAAYVGDSPASLMRVGETSPGFEWDCDFLPHLRVGTTAGVTYRFAVDGAGAPGGQVAA
                     GRIFMRLQVTPNYPQNDSFGTPYELVGVPGRVWNWDLPTDGTTKEFGEPAHAGDAGGS
                     SLWFRFVPRESGVVEIDTCASDFDTLLAAYTGDSVRALTPVAANDDSVGPACPGTNRS
                     ALRFHVSAGVVYRVAADGKGGAEGKLAIANRLIPDSQLTPPNTFITRVKLDPKRRLSR
                     FFFRSDKLGSRFFCQLDRGPRRRCRSPVTFTKLAAGMHVLRVTAEDFAGNVDPSPRVR
                     RFRVQPPPPPGAS"
     gene            5171..5596
                     /locus_tag="VFB52_03095"
     CDS             5171..5596
                     /locus_tag="VFB52_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZO05345.1"
                     /translation="MDELYRDQILEHYKRPHNFGALDSPDLEFEDTNPFCGDEQHVTI
                     KLDGSDRVAEVAFEGKGCAISTAATSLLTDELVGMSREELLKLPKDFVLELLGIEISA
                     TRMKCAMLGLKIVKSASLGQPAEWEDEGETGSPSEAAAI"
     gene            5610..6002
                     /locus_tag="VFB52_03100"
     CDS             5610..6002
                     /locus_tag="VFB52_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HZO05346.1"
                     /translation="MEGTADLERFVAAQDQGGTYERALAELRAGSKRSHWMWFVFPQE
                     KRLGHSPMAQRYAIESLDEAVAYAAHPVLGPRLRECCRALLDLEGSPTAVAVLGEIDA
                     LKLCSSMELFARADPDEPLFRAVRERYC"
     gene            complement(5999..6796)
                     /locus_tag="VFB52_03105"
     CDS             complement(5999..6796)
                     /locus_tag="VFB52_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05347.1"
                     /translation="MSERTSYTPGTPCWVDLATPDIDAAEKFYGPLFGWEIPELPNSA
                     EMGGYRRAKKGGKDVAGVYPMMEEGQHPAWATYVAVTDAAATGALIEQNGGTAIVAPM
                     DIGMGHMAVFADPAGAVFGLWQPVTFAGAELVNEAGCFGWNELGTRDTAGAKQFYPAV
                     FGWGTEEEESERAGTYTVWTVGEAMVGGMIDMNALDMPPEVPPNWLVYFMVENTDAAV
                     EQVNAGGGGVRMGPIDIPVGRFAVVADPAGAVFAIMQPSEATLANMP"
     gene            6989..7315
                     /locus_tag="VFB52_03110"
     CDS             6989..7315
                     /locus_tag="VFB52_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZO05348.1"
                     /translation="MTKTAGTTISVCPAAELPPGAMKLVEHDGMKIGVFNCEGTIYAI
                     EDRCSHDDGPLAEGEFDAATCTVECPRHGSLFDLTTGRPKTLPAFKPVQTFPTAVADD
                     TITLEV"
     gene            7317..>7571
                     /locus_tag="VFB52_03115"
     CDS             7317..>7571
                     /locus_tag="VFB52_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZO05349.1"
                     /translation="MATPEAVAEKQELAGLNANYEEKFGFHDPESGYAYKAPKGLSRE
                     VVESISDYKDEPAWMREFRLKSLEHFYSRPTPNWGGNLAQI"
BASE COUNT         1204 a         2724 c         2559 g         1084 t
ORIGIN      
        1 acccgggtcc agggcggcgt caccaaggcc ttggccggga cgacgctggc agagctggtc
       61 gagttctcgc agcccggcgg gcgcagcgag gccgacgtcg tcaaggccgc ctgagacttc
      121 ccaataccga aacggagtga gcattggctg agttggagat aaggaacctg cacgcgaagg
      181 tcgaggagaa ggagatcctc aagggcctcg acctcgacgt cgagaagggc aaggtccatg
      241 ccctgatggg cccgaacggc tccggcaagt cgacgctcgc caacgtgatc atgggccacc
      301 ccgcctacga ggtgaccgag ggcacgatca gcttcaaggg cgaggacatc accgaggccg
      361 accccgacga gcgctcgcag gccggtctct tcatggcctt ccagtacccc gtcgcgatcc
      421 ccggcgtctc ggtctccaag tacctgcgga tgatcatcaa cgccaagcgc gaggcgcacg
      481 gcgaggagcc gatcaagatc aaagacttcg cccgcgaggc gcaggaggcg atggcgctgg
      541 cgaacatccc caaagacttc tccagccgct acctcaacga cggcttttcg ggcggcgaga
      601 agaagcggat ggagctgctg cagctggcgc tgctgcggcc ggagatcgcg gtgctcgacg
      661 agaccgactc gggcctcgac atcgacgctc tgcggaccgt cgccgacggg gtcaatcagt
      721 tcgccggccc cgacatgggc gtcctgatca tcacccacta ccagcgcatc ctgcacatgg
      781 tgaagccgga cttcgtccac gtcatgttcg agggccggat cgtcaaagaa ggcggcccgg
      841 agctggtcgg cgtgctggag gagaaaggct acggctggat cgccgaggag gtcggggcgg
      901 gcgccagcgc ctagccccgg tcgggagatg gagccggtga gcacggagag cgtctccccc
      961 gtcgcggcgc ccttcccgat cgagaaggtt cgcgccgcct tcccggcgct ggagcgcgag
     1021 atcggcggca agccggtcgc ctacctcgac agcggcgcca gctcgcagcg ggtgctcgcc
     1081 tcgatccagg cggtcgaccg ctacgagcgc cgccaccact cgaacgtcca ccgcggctcc
     1141 cacacgctct cggccgaggc gaccgaagcc tacgagggcg cccgggccac ggtcgccgac
     1201 cacatcggcg ccggcgaccg ccgcgaggtc gtcttcgtcc gcaacgcgac cgaggcgatc
     1261 aacctggtcg cgcgcgcctg gggcgacgcc aacgtcggcg ccggcgaccg gatcgtgctc
     1321 accgagatgg agcaccactc gaacatcgtg ccctggcagc agctggccga gcgggtcggg
     1381 gcggagatcg actgggcgcc gatcgacgag gaggggctgc tcgacatgga ggccttcgcg
     1441 gcgctgctag agcgcggccc gaagctggtc gcggtcaccc acgtctccaa cgtgctcggg
     1501 accgagaacc cgctgaccga gatcgcccgg ctcagccacg acgccggtgc cctggtgctc
     1561 gccgacggcg cccagtcggc gccgaagctg ccactcgaca tggcggcgct cggggtcgac
     1621 ttctacgcga tcaccggcca caagctctac gcgccaaccg ggatcggggc gctctggacc
     1681 cgcctcgacc tgctgcgcga gatgccgcct ttcctcggcg gcggctcgat gatccgcaag
     1741 gtgacccgcg agggcaccac ctatgcggac gtgcccgccc gctttgaggc cggcacgccg
     1801 gcgatcacgc aggcagtcgg gatggcggcg gcgctgcgct ggctcgacgc cctcggaatg
     1861 gagacggtgc gcaaccacga gcgtcagatc gccgactacg ccctcgcccg ggtcacagag
     1921 gtccccggcc tcaaggtctt cggcccgccg cgcggacccg agcggctcgg cccggtctcc
     1981 ttcgagatcg agggcatcca cgcccacgac gtctccgaga tcctcgaccg ccacggcgtc
     2041 gccgtgcgcg ccggccacca ctgcgcccag atcctcatgg accgactcgg cgtcgccgcc
     2101 accgcgcgcg ccagcttcgg cgtctacacg acgcccgagg agatcgaccg cctggtcgac
     2161 ggcctcgaag acgcccgccg cgtcttcggg ctggacgctt aggccggccc ggccttcgca
     2221 aaccggttcg gcgcctacac tgctccgacc gaccgatgct cgtcctgctt cgcaccatcg
     2281 ccgtctcagc tctgctttcg ctcgcggtgc ttgccagcgc cgcgtcggca gcgccgcggc
     2341 tgatcgcgct cggcgccggc cccaacgtga acgtcctctt cagcttcgat gccgacaatc
     2401 ccggcgcgac gaccgctgtc ccggtcacgg ggttgggctc gtcctcgctg gtcggaatcg
     2461 accgccgccc cgccgacggc ctgctgtacg ggctcggcgt cgacggcaac gccgcgacgc
     2521 tgttcaggct cgaaccgacg accggcaccg caagttcggt cggcagcgtc acgatcacct
     2581 cgctcaccgg tgccagcgcc tacggcgtcg acttcaaccc ggtcagcgac cggctgcggg
     2641 tggtcaacga cctcgcttcc ggcgtcggca ccaacgtcaa caacttccgg atccgccccg
     2701 cagcgaacct tctcgccggg attgactcag acctcgactt cagcggcgct ccgggcagcg
     2761 gcagcgaggt cgcgatcgcg cacgatcgcg gtgtcggggc gagcgcgacc acccttttcg
     2821 gagtcaccgc gatcggcgac atgctcgtcc gccaaggcgg cgtcgatggg acgccgagcc
     2881 cgaatggcgg cgcgatctcc gcgatcggcc cgctcggggt cgacatcacc gcgaacgccg
     2941 gcctcgacat cgacccgacc agcggcgagg ccttcgcggt cttcaccgtc gccggcgagt
     3001 cgagcctcta ccgggtcagc ctggcgaccg gtgccgcaac gctggtcggc aaagtcggga
     3061 ccggcaccgt ctcattcggc ggcctcacga tcgacccccc cgttgccggt ccgccgcccc
     3121 cggccgagcc gcagctcgag tcactgagcc tgcgcccgcg agcgtttgcg actgcgaaca
     3181 tcggcggcgc gatcctcagc aaagtccgca tcgggaccac ggtcagctac tccctctcgg
     3241 ccgccgcgac gaccgccttc acggtcgagc gcaagagcac cggccggcgg gtcggcaagg
     3301 cctgcaaaaa gctgacgcgc ggcaatcgag accgcaagcc ctgcccgctc ttcaagccgc
     3361 tcaaagccgg cttcacgcac gctggcgcgg ccggcgccaa cgccttcagg ttcaccggcc
     3421 ggatcggcgg caacgcgctg agaccaggcg cctaccggct ggtcgcccgc gccggttcct
     3481 cgtccgcccg cacgaccttc acggtcgtgc cgcgtggccg aacccgctag ctcgcacccg
     3541 ggggcggcgg cggctgaacc cgaaagcgcc gcacgcgcgg gctcgggtcg acgttgccgg
     3601 cgaagtcttc ggcggtgacc cggagcacgt gcatgccggc cgcgagcttg gtgaaggtga
     3661 ccggtgagcg gcagcgacgc cgcggaccgc gatcgagctg acagaagaag cgcgacccga
     3721 gcttgtcgga gcggaagaag aagcgcgaga ggcgccgctt ggggtcgagc ttgacccggg
     3781 tgatgaaggt gttcggcggc gtcagctggc tgtcggggat cagccggttg gcgatcgcca
     3841 acttgccttc cgccccgccc ttgccgtcgg cggccacgcg atagacgaca ccggcgctga
     3901 cgtggaaccg cagggcgctg cggttcgtcc ccgggcaggc cgggccgacg ctgtcgtcgt
     3961 tggccgccac cggcgtcagc gccctcaccg agtcgccggt gtaggcggcg agcagggtgt
     4021 cgaagtcgct cgcgcaggtg tcgatctcaa cgacgcccga ttcgcgcggc acgaacctga
     4081 accagagcga ggagccgccg gcgtcgccgg cgtgggcggg ctcaccgaac tccttggtcg
     4141 tgccatcggt cggcaggtcc cagttccaga cccgcccggg aacgccgacc agttcgtagg
     4201 gcgtcccgaa gctgtcgttc tgagggtagt tgggggtgac ctggagccgc atgaagatcc
     4261 ggccggcggc gacctgcccg ccgggcgcgc cggcgccgtc gaccgcgaag cggtaggtga
     4321 cgccggccgt ggtgccaacg cgcaggtggg gcaggaagtc gcagtcccac tcgaagccgg
     4381 gagaggtctc tccaacccgc atcagcgagg cgggcgagtc gccgacgtat gccgccacgc
     4441 gagcgtcgaa gctgctgcca caggtggaga gctcggcgac gccgtcgctc ggcgcggtcc
     4501 acgagtacca gacggtggcg ccgccggaac cggccgggac cacctcccgc ggctcggtgg
     4561 tggcgccgac gttgtggcct tcggcgaggt cgtccgtgcc gccgagccag tcgctgttgg
     4621 caagggtgtc gttgaagcgc ggctgacttt cgtcgaagct gagctggaag ttgttggcgc
     4681 cgccttcggc ggcgaccgcg atccggtagg tcgtccctgc caccgcccgg aagctggcct
     4741 gccattccgg ggcgtagccg cagccaccgc cggctccgac gctggtccga gacaccagcg
     4801 tcagcgcatc gacggcgctg cccgtgtaga ccgcctcgac ggcgttgacc ggaccgccgc
     4861 agcggagcac cgtgacccag cgcgagaccg gcgcctgcca ggagtaccag accgtcgtcg
     4921 tcaacccggg cccgtggacg ggctcgccgg gctcggtcgt cgcttcgccg acgttgccga
     4981 aagcgctggg gtcttcgccg gtgagcgtct cggcgtcggc gaagtcgtcg ttggcgggcg
     5041 gcgcggcggc tgcgggcgca gcacagacga gcgccacgag cacggcgagc acggctccga
     5101 tcccccgctg cctgtgcatg agaccgcgca ggatagggac cgtggacccg cttcgctagc
     5161 ctcgatggcg atggacgagc tctaccgcga ccagatcctc gagcactaca agcgcccgca
     5221 caacttcggc gcgctcgaca gccccgacct cgagttcgag gacacgaacc cgttctgcgg
     5281 cgacgagcag cacgtgacga ttaagctcga cggctccgac cgggtggccg aggtcgcctt
     5341 cgagggcaaa ggctgcgcga tctccaccgc cgccacctcg ctgctcactg acgagctcgt
     5401 cgggatgtcg cgcgaggagc tgctgaagct gccgaaagac ttcgtcctcg agctgctcgg
     5461 gatcgagatc tcggcgacgc ggatgaagtg cgcgatgctc ggcttgaaga tcgtgaagag
     5521 cgccagcctc ggccagcccg ccgaatggga ggacgaaggc gagaccggca gcccgagcga
     5581 agccgcggcg atctagcgcc gccgccgccg tggagggcac cgccgacctg gagcgcttcg
     5641 tcgccgccca ggaccagggc gggacgtatg agcgggcgct ggccgagctg cgcgccgggt
     5701 ccaagcgcag ccactggatg tggttcgtct tcccccagga aaagaggctg ggccacagcc
     5761 cgatggcgca gcgctacgcg atcgagtcgc tcgacgaagc ggtcgcctac gccgcccacc
     5821 cggtgctcgg cccgcgcctg cgcgagtgct gccgggcgtt gctcgacctc gaggggagcc
     5881 cgaccgcggt cgcggtgctc ggcgagatcg acgcgctgaa gctctgctcc tcgatggagc
     5941 tgttcgcgcg cgccgacccc gacgagccgc tcttccgagc ggtccgcgag cgctactgct
     6001 agggcatgtt cgccagcgtc gcctcgctcg gctgcatgat cgcgaagacg gcaccggccg
     6061 gatcggcgac gacggcgaag cggccgaccg ggatgtcgat cgggcccatg cgcacgcctc
     6121 ccccacccgc gtttacctgc tcgaccgcgg cgtcggtgtt ctcgaccatg aagtagacga
     6181 gccagttggg cggcacctcg ggcggcatgt cgagcgcgtt catgtcgatc atcccgccga
     6241 ccatcgcctc gccgaccgtc cagacggtgt aggtgccggc gcgctcggac tcttcctcct
     6301 cggtgcccca gccgaagacc gccggataga actgcttggc tccggcggtg tcgcgggtgc
     6361 caagctcgtt ccagccgaaa cagccggcct cgttgaccag ctccgcgccg gcgaaggtga
     6421 ccggctgcca gagcccgaac acggccccgg ccgggtcggc gaagacggcc atgtggccca
     6481 tcccgatatc catcggcgcg acgatcgcgg tgccgccgtt ctgctcgatc agggcgccgg
     6541 ttgcggcggc gtcggtgacc gcgacgtagg tcgcccaggc cgggtgctgg ccctcttcca
     6601 tcatcgggta gaccccggcg acgtccttgc cgcccttttt cgcccgccgg tagccgccca
     6661 tttcggccga gttcggcagc tccgggatct cccagccgaa cagcgggccg tagaacttct
     6721 cggccgcatc gatgtccggc gtggccaaat cgacccagca gggcgtgccg ggggtgtaac
     6781 tggtcctctc gctcatcttc tccctttctt tgtgtggcta ctgcctataa gaccacccga
     6841 ccgcccaaaa ctcatcgttc agttgtgaaa tcgcttcctg gcgtcttcgc caggcacggc
     6901 cggtcacgct cgcccgctaa aatccgagtg cagaagtagg attaagtctc atcccgcact
     6961 agccacccct atggggagcc cggcctctat gactaaaacc gcaggaacga ccatctccgt
     7021 gtgcccagcc gccgagctcc cgcccggcgc aatgaagctg gttgagcacg acgggatgaa
     7081 gatcggcgtc ttcaactgcg agggcacgat ctacgcgatc gaggaccgct gctcccacga
     7141 cgacggaccg ctcgccgagg gcgagttcga cgccgccacc tgcaccgtcg agtgcccccg
     7201 tcacggctcg ctcttcgacc tgaccaccgg caggccgaaa acgctccccg ctttcaagcc
     7261 ggtgcagacg ttcccgaccg ccgtcgcgga cgacaccatc accctggagg tctgaaatgg
     7321 caacgcccga ggcagtcgcc gagaaacaag aacttgctgg cctgaacgcc aactacgagg
     7381 agaagttcgg cttccacgac ccggagtccg gctacgccta caaagccccc aaggggctct
     7441 cgcgcgaggt cgtcgaatcg atctccgatt acaaggacga gccggcctgg atgcgcgagt
     7501 tccgcctcaa gtcgctcgag cacttctact cgcgcccgac cccgaattgg ggcggcaacc
     7561 tggcgcagat c
//
LOCUS       DASRIG010000113         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_827140, whole genome shotgun sequence.
ACCESSION   DASRIG010000113 DASRIG010000000
VERSION     DASRIG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827140"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFB52_03120"
     CDS             <1..814
                     /locus_tag="VFB52_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HZO05350.1"
                     /translation="VLGRRGPVQAAFTNPEIKELGEMEDAEPIVDPSEMKLDPASAAF
                     LESDEADKTTRVNVETLREFSERTPEGKKQRFVLRFLSSPVEIKGDGKVESIVIGRNE
                     LVEEGGTLRAKDTGEREELECGLILRSVGYTGIPIEGVPFDEKRGLILNEGGRVLDSH
                     DAGHKVGHYTAGWIKRGPSGVIGTNKKDALETVQHLFADVESQTLLAPENPAPEAIEE
                     LLAEKGTRFVSFEDWQAIDQAEVGRGEPHGRPRVKFVRVEEMLGTLGEKIGG"
     gene            complement(821..1711)
                     /locus_tag="VFB52_03125"
     CDS             complement(821..1711)
                     /locus_tag="VFB52_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO05351.1"
                     /translation="MQREERRFRSGGEECAAWLFVPDGLDEPAPCVVLGSGFSCVRDQ
                     RLDVLAERFAAAGFVALAFDYRYFGDSGGEPRALVSAARQRQDWRAAIAYARALEQVD
                     TRRVALWGFSLGGGHVQALARADREVAAAICVCPLVDGVRTLLHVGGPAHLARLFAAG
                     ARDGLRALRRAEPHRIRAAGPPGSGAVLARPSALAEFGAVSPPGSTWRNEACARAALA
                     PPYLLLERKARRIRCPILFCIADGDEINPPALGIEASRNAPAGELRRYPGGHFGPLHE
                     PVFEPAVADQLDFLDRHLRG"
     gene            1759..1992
                     /locus_tag="VFB52_03130"
     CDS             1759..1992
                     /locus_tag="VFB52_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HZO05352.1"
                     /translation="MVDLAELKTMIEAALPGASVEVIDEDGGGQHLRAEVSAPQFEGL
                     SRIDQHRLVKAAVKPRFDDGTVHALSVKTSLPS"
     gene            2072..2308
                     /locus_tag="VFB52_03135"
     CDS             2072..2308
                     /locus_tag="VFB52_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05353.1"
                     /translation="MESMRSTWTDSRLDDLKDRVDVRFDEMGGRISELSSRVDNQSDR
                     IDSLQHAIIVGGGGLIAACVAVLAAILGLIATQL"
     gene            2341..2727
                     /locus_tag="VFB52_03140"
     CDS             2341..2727
                     /locus_tag="VFB52_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZO05354.1"
                     /translation="MADPELKSKVEELIAANPILLFMKGTPDAPRCGFSLRVVGVLDQ
                     MDVEYGAIDVLPALQPLREVTTEIADWQTFPQLYVNGELLGGCDIVEEMFESGELAEA
                     LGVEQPEQASAPQAAAPAQSPPLQIE"
     gene            complement(2728..3099)
                     /gene="erpA"
                     /locus_tag="VFB52_03145"
     CDS             complement(2728..3099)
                     /gene="erpA"
                     /locus_tag="VFB52_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZO05355.1"
                     /translation="MSDTPEAAVTLSAKAVEKIGELLGSQAEADQQALRVAVRGGGCS
                     GFQYALAFDKAKDDDHVFEVDGVAVIVDKVSMQFVFGSEVDYVEGLQGAGFQVNNPNV
                     VAACGCGSSFQVKEDAAASAV"
BASE COUNT          559 a         1077 c         1126 g          444 t
ORIGIN      
        1 cgtgctcggc cgccgcggcc cggttcaggc ggccttcacc aaccccgaga tcaaggagct
       61 cggggagatg gaggacgccg agccgatcgt cgacccgagc gagatgaagc tcgacccggc
      121 cagcgccgcc ttcctcgagt ccgacgaggc cgacaagacg acccgagtca acgtcgaaac
      181 cctgcgcgag ttctccgagc gcacgccgga gggcaagaag cagcggttcg tgctgcggtt
      241 cctctcctcg ccggtcgaga tcaagggcga cggcaaggtc gaatcgatcg tgatcggtcg
      301 caacgagctg gtcgaggaag gcgggacgct gcgcgccaag gacaccggcg agcgcgaaga
      361 gctggagtgc ggcctgattc tgcgctcggt cggctacacc ggcatcccga tcgaaggcgt
      421 accgttcgac gagaagcggg gcctgatcct caacgagggt ggccgcgtgc tcgactccca
      481 cgatgccggc cacaaggtcg gccactacac cgccggctgg atcaagcgcg gcccgtccgg
      541 ggtgatcggg acgaacaaga aagacgcgct ggagacggtc cagcacctgt tcgccgacgt
      601 cgagtcgcag acgctgctgg cgccggagaa cccggccccc gaggcgatcg aggagctgct
      661 cgccgagaag ggcactcggt tcgtcagctt cgaggactgg caggcgatcg accaggccga
      721 ggtcggccgc ggtgagccgc acggccgccc gcgggtcaag ttcgtgcgcg tcgaggagat
      781 gctcggcacg ctcggcgaga agatcggcgg ctagaccgcc tcagccacgc aggtggcggt
      841 cgaggaaatc gagctggtcg gcgacggccg gctcgaagac cggctcgtgc agcggcccga
      901 agtgaccgcc cgggtagcgc cgcagctcgc ccgcgggtgc gttgcggctg gcctcgatgc
      961 cgagcgccgg cgggttgatc tcgtcgccgt cggcgatgca gaagaggatc gggcagcgga
     1021 tgcgccgggc tttcctctcc agtaggagat agggcggcgc caaggcggcg cgggcgcagg
     1081 cctcgttgcg ccaggtcgag ccgggcgggc tgaccgcccc gaattcggcc agggcggagg
     1141 gacgggcgag caccgcgccg gatcccggcg ggcccgcagc ccgaatccgg tgaggctcgg
     1201 cccggcgcag cgcgcgcaga ccgtcgcggg cgccggcggc gaagagcctg gccagatggg
     1261 cggggccgcc gacgtggagc agggtccgca ccccgtcgac cagcgggcag acgcagatcg
     1321 ccgccgcgac ctcgcggtct gcgcgcgcca acgcctgcac gtgcccgccg ccgagcgaga
     1381 agccccagag cgcgacccgg cgtgtgtcga cctgctcgag cgcgcgcgca taggcgatcg
     1441 cggcgcgcca gtcctggcgc tggcgcgcgg cgctgaccag tgcgcgcggc tcgccgccgc
     1501 tgtcgccgaa gtaccggtag tcgaaggcga gggcgacgaa accggccgcc gcgaaccgct
     1561 cggctaagac atcgaggcgc tggtcgcgta cgcaagagaa cccggagccg agcaccacgc
     1621 agggcgcggg ctcgtcgaga ccgtcgggga cgaacagcca ggcggcgcac tcctcgcccc
     1681 ccgaccggaa gcgtcgctcc tcccgctgca tctcccgatc ctagtccggc gccgtctccg
     1741 catccataga atcagcccgt ggtcgatcta gctgagctga agacgatgat cgaggccgcc
     1801 ctgccgggag cctcggtcga ggtcatcgac gaagacggcg gcggccagca cctgcgtgcc
     1861 gaggtctcgg ccccccagtt cgagggactc tcccggatcg accagcaccg gctggtcaag
     1921 gccgcggtca aaccgcgctt cgacgatggc accgtgcacg cgctgtcagt gaagaccagc
     1981 cttccgagct gaagtcgcgc ccacgcgggc gtagacaaaa agttacactt ctgtgcgccg
     2041 caggtgcgct ttttctgtct acgctggagg catggagtcg atgcgatcaa cctggactga
     2101 cagccgtctc gacgatctca aggatcgagt ggatgtccgc ttcgacgaga tgggcggccg
     2161 aatctccgaa ctcagtagcc gagtcgacaa ccaaagcgac aggatcgact cgctccagca
     2221 cgcgatcatc gtcggcggcg gcggcctgat tgccgcgtgc gtcgccgtgt tggcggcgat
     2281 cctcgggctc atcgcgacgc agctctaggc cgacgagcgc acctagctag tcttctgccc
     2341 atggccgacc cagagctgaa gtccaaagtc gaagagctga tcgccgccaa cccgatcctg
     2401 ctgttcatga aagggacccc ggacgcaccc cgctgcggat tctcgctgcg ggtcgtcgga
     2461 gtgcttgacc agatggacgt cgagtacggg gcgatcgacg tgctgccggc cctgcagccg
     2521 ctgcgcgaag tgaccaccga gatcgccgac tggcagacct tcccgcagct ctacgtcaac
     2581 ggcgagctgc tcggcggctg cgacatcgtc gaggagatgt tcgagtccgg cgaactcgcc
     2641 gaggccctcg gcgtcgagca gccggagcag gcaagcgcac cgcaggcagc cgcacccgcg
     2701 cagtcacccc cgctgcagat cgaatagcta gacggcggag gctgctgcgt cctctttcac
     2761 ttggaaagag gaaccgcatc cacacgccgc aacgacgttc gggttgttga cctggaagcc
     2821 cgcgccttgc aggccttcca cgtagtcgac ctcggagccg aagacgaact gcatgctgac
     2881 cttgtcgacg atgacggcga cgccgtcgac ttcgaagacg tggtcgtcgt ccttggcctt
     2941 gtcgaaggcg agcgcgtact ggaagcccga gcaaccgccg ccgcggaccg cgacgcgcag
     3001 cgcctgctga tcggcctcgg cttgagagcc gagtagctcg ccgatcttct cgacggcctt
     3061 ggcggaaagg gtgactgctg cctctggggt atcgctcact tcacaaagtg tagctaggtc
     3121 ggaggatccg ctccggattt gcggcgggac ccgggagcgg atcagcgaat agactcggct
     3181 ggttgaccgg gagcgagaag accgca
//
LOCUS       DASRIG010000114         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2929716, whole genome shotgun sequence.
ACCESSION   DASRIG010000114 DASRIG010000000
VERSION     DASRIG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929716"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFB52_03150"
     CDS             <1..453
                     /locus_tag="VFB52_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05356.1"
                     /translation="WDLRSTARHPEGWTSADAAGLPIFPGLVRYDEVAAGQLDHAIRV
                     TFESTRDAYVHPAVHCAGDTANAAAPPMGLRLRLKAGFGLGRFSGGAKTIALAMKRYG
                     LIVADNGSNWYFSGSSDKRWDDENLNQLKGIPGSAFEVVKSAARPHAC"
     gene            513..1346
                     /locus_tag="VFB52_03155"
     CDS             513..1346
                     /locus_tag="VFB52_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05357.1"
                     /translation="MADLSTRSRRGALACALTALALLAAPATSPAANSGGDENPLDVL
                     GKTAVAALGSAGANHALDRITENLAMLAAQERIEQRAKNEAKNIDRTPEEEPEVDYSK
                     MDPSELPPASAGTNGTEHELSAKSKREGIFARMPAGADPESTAFLLTGIALPPPNAPD
                     VVKDVINNANLIVGRPYIWGGGHGSFYSYGYDCSGAVSFALFGGGLIPRPATSGELEG
                     YGEPGPGKWITIYANASHTFAEIAGLRWDTSGTERGTGPRWHVDARSADGYVVRHPPG
                     L"
     gene            1425..1709
                     /locus_tag="VFB52_03160"
     CDS             1425..1709
                     /locus_tag="VFB52_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HZO05358.1"
                     /translation="MKFATIYRPKYPAPPELIPDLLKEMGAWMQRYADRLENVQFFIG
                     GGGFGTVDSDDAALLAKMITEHPFTIYSDVEIKPLVDPATALGILQETYS"
     gene            complement(1711..2088)
                     /locus_tag="VFB52_03165"
     CDS             complement(1711..2088)
                     /locus_tag="VFB52_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05359.1"
                     /translation="METPTEEESESASVTETPEVTEEIAPTPVAEKPKPKPSLGGGAA
                     VLGVSTSSAEEAAKALAPSIPVADLADQGGSGSNLALLVFACLLALALAAGAGRFGLQ
                     RRRRRRRWDREAAEWQAAVRRLH"
     gene            2446..>2630
                     /locus_tag="VFB52_03170"
     CDS             2446..>2630
                     /locus_tag="VFB52_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05360.1"
                     /translation="MKPAVTYRRRGLAGFVAAAILTILGLHATVATAAPTLGLSATDA
                     VIGNTIQATAQLSAIEGS"
BASE COUNT          435 a          983 c          845 g          367 t
ORIGIN      
        1 tgggacctgc gctcgaccgc ccgccacccc gagggctgga cctccgccga cgccgccggg
       61 ctgccgatct tccccggcct ggtccgctac gacgaagtcg ccgccggcca gctcgaccac
      121 gcgatccggg tcaccttcga gagcacccgc gacgcctacg tccaccccgc ggtccactgc
      181 gccggcgaca ccgccaacgc cgccgccccg ccgatgggcc tgcgcctgcg gctgaaggcg
      241 ggcttcgggc tcggtcgctt cagcggcggc gcgaagacga tcgcgctggc gatgaagcgc
      301 tacggcctga tcgtcgccga caacggctcc aactggtact tcagcggcag cagcgacaag
      361 cgctgggacg acgagaacct gaaccagctg aagggaatcc caggcagcgc cttcgaggtc
      421 gtcaagagcg ccgcccgccc gcacgcctgc tgaaacaaaa ccgcaacaat cgcccgcgcg
      481 gggcatctct aaagtttccg agctagggac ctttggcgga tctttcgaca cgcagccgtc
      541 gcggcgcact tgcctgcgcg ctgaccgcgc tcgccctgct cgcggcgccg gccaccagcc
      601 cggccgccaa ctccggcggg gatgagaacc cgctcgacgt gctcggcaag accgcggtcg
      661 cggcgctcgg ctcggccggc gccaaccacg cgctcgaccg gatcaccgag aacctggcga
      721 tgctcgccgc ccaggaacgg atcgagcagc gcgccaagaa cgaagcgaag aacatcgacc
      781 gcacgccgga ggaagagccg gaggtcgact actcgaagat ggacccctcc gaactgccgc
      841 ccgcctcggc cggcaccaat ggcaccgagc acgagctcag cgccaagtcc aagcgcgagg
      901 ggatcttcgc ccgcatgccg gccggcgccg acccggagtc aaccgccttc ctcctcaccg
      961 ggatcgccct gccgccgccg aacgcgccgg atgtcgtcaa ggacgtgatc aacaacgcca
     1021 acctgatcgt cggccgcccc tacatctggg gcggcggcca cggctccttc tactcctacg
     1081 gctacgactg ctcgggcgcg gtcagcttcg ccctcttcgg cggcggcctg atcccccgcc
     1141 ccgcgacctc cggcgaactc gagggctacg gcgagcccgg ccccggcaag tggatcacga
     1201 tctacgccaa cgcctcccac acctttgccg aaatcgccgg cctgcgctgg gacacctccg
     1261 gcaccgaacg gggcaccggc ccccgctggc acgtcgacgc ccgctcggcc gacggctacg
     1321 tcgtccgcca cccgcccggc ctctaaaacg ttcttgaacg tttacgtggt gcgctgcggt
     1381 cccgtttggg caggggataa ctgcaccgct acggaggaga gaccgtgaaa ttcgcgacga
     1441 tttaccggcc caaatatccg gcgccgccgg agctgatccc ggatctgctc aaagagatgg
     1501 gggcatggat gcagcgctat gccgaccggc tcgaaaacgt ccagttcttc atcggcggcg
     1561 gcggcttcgg caccgtcgac agcgacgatg ccgccctgct ggcgaagatg atcaccgagc
     1621 acccgttcac gatctactcg gatgtcgaga tcaagccgct ggtcgacccg gcgaccgcgc
     1681 tcgggatcct ccaggagacc tactcctgaa ctagtgaagc cgccgcacgg cggcctgcca
     1741 ttcggccgct tcccggtccc agcgccggcg gcgccgccgg cgctggaggc cgaaccggcc
     1801 ggccccggcc gcgagcgcga gggccagcaa gcaggcgaac accagcaggg cgaggttgga
     1861 gccggagccc ccctggtcgg cgaggtcggc gacggggatc gagggcgcca gggccttggc
     1921 cgcctcctcc gcggacgagg tcgagacccc gagcacggcc gcgccgccgc caagcgaggg
     1981 cttcggcttc ggcttctcgg cgaccggggt gggagcgatc tcttcggtga cctcgggggt
     2041 ttcggttacc gatgcgctct cgctctcctc ttcggtcggc gtttccacca ctggcgcggg
     2101 tggcggcgcc ggggtggaac cggagcccga aggctcgggc tcggcggccg gagccgaatc
     2161 gccaccgctg ccgagcgagc tgccgctctc gacttccgag tccccttcgg aaccttccga
     2221 acctggattc ggttcgaccc agccgccgtt cggaggcgtt gcaccggatt ccggggcggg
     2281 ttcttcggcc gcgagcgccg tcgccgggag accaaggacc agcaccgcca acgcgaccaa
     2341 aaggaacgct ggaaggaacc gacggtggtt ttttttcatt agcgtccgag ccaagtgatt
     2401 gcgagcgtga tggacggata tcctagtccg gttccccagc ttgcgatgaa acccgccgtt
     2461 acatatcgcc gacgcgggct tgcaggcttc gtcgccgccg cgatcctcac catcctcgga
     2521 ctgcacgcga ccgtcgccac tgcagccccg accctcggcc tgagcgcaac cgacgccgtg
     2581 atcggcaaca cgatccaggc gaccgcccag ctctctgcaa tcgaaggctc
//
LOCUS       DASRIG010000115         4585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1114045, whole genome shotgun sequence.
ACCESSION   DASRIG010000115 DASRIG010000000
VERSION     DASRIG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114045"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1125)
                     /locus_tag="VFB52_03175"
     CDS             complement(142..1125)
                     /locus_tag="VFB52_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZO05361.1"
                     /translation="MARRVSVFAALLVALALATVASLALGAVKVPFSDVIDILLGKGP
                     GGPEQQIVEALRLPRTVEAMVVGAALGIAGAILQGALGNPLASPDVIGVTGGAGFGAI
                     LIIILFPGSIALLPVGALFFGLLAATLVFLFAWSGRNRGSVGKVILAGIAMLSLFTAG
                     TTGLLAAYPERAPSAIFFIAGGLTSSGWEELQTIWPYFAVGFIFALLLIRPLDRLALG
                     DDVAASLGSRPQLIRLAAGIAAALLAAASAAIAGLLGFLGLVVPHVVRMAGGSSSHTF
                     VVPASAIGGAALLAGADTLARTLRAPIEMPVGPFMVILGVPLFLWLLRKAV"
     gene            complement(1131..2006)
                     /locus_tag="VFB52_03180"
     CDS             complement(1131..2006)
                     /locus_tag="VFB52_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO05362.1"
                     /translation="MKRLATLAALAAALIALALPAVTGAAQSGNDVPKRIVALTPFAA
                     NTLAKLGVTPVGVGETLGGQTRLSPKLKNVTVLPLSHPNGPNLEQLASLRPDVVLSSR
                     TWVKGNQAMEDLGIEVRVYEPLKIGRAYRDTYRIGQLVGKQRQARAQLKAMKKSVEAA
                     TKGIKSHPKVMLLLGVGRTPFTMLSNSWGGEIVIKAGGELLTGGVDSDSGFARISDEL
                     VVAENPDVIIAVPHANASDIPSLTEYLKDNPAWQSTSAAQNGRIYVSVDNSLLQAGTD
                     IARTIRKVRSAYLKN"
     gene            complement(2003..2293)
                     /locus_tag="VFB52_03185"
     CDS             complement(2003..2293)
                     /locus_tag="VFB52_03185"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZO05363.1"
                     /translation="MMNVVKVDEDKREAFEQQFLGRESHVHKAPGFAGFELLRRDRDG
                     EYVVLSRWESEEAFQGWLNSELFKLSHQHADGQLAHSSEVRNYDVIDARVPA"
     gene            complement(2399..3208)
                     /locus_tag="VFB52_03190"
     CDS             complement(2399..3208)
                     /locus_tag="VFB52_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZO05364.1"
                     /translation="MLIELQSHSTVSDGQLPPAEVVEKAAEAGVTTLALTDHDGVAGV
                     PAAAEAAERLGIELVPAIEMSCVHPYAEDLHICGYWIDLPTMEPICARAQQERRNRAG
                     EIVENLRREGFDLTLEDAIREAGDADSIGRPHIAKAAGASGDLGPFFEEYLVPGAKAF
                     VPRRWPDAAEAIELIRGAGGVAVIAHPYWDISDPGEVEDLIRSLQPDGIETYYPPHTR
                     EQTAHLLGLCQELGLYPTASSDYHGPTHKTFAEFGVYDTYGLGEPVVPPRP"
     gene            3273..4295
                     /locus_tag="VFB52_03195"
     CDS             3273..4295
                     /locus_tag="VFB52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HZO05365.1"
                     /translation="MLKRSLFLGALLALLLAVPAQAKLLYVGDKGTAPVGKVSCPAGQ
                     GCAVQTPKRVKAKIGTKSFWARVLAPKRVAAGKQGTVRVKFGGGALGDLAGRTTTVKV
                     KAVVRQDGVARTRLLQVRLRRAALPAGGSDNPSSGPLGNEPPLLVRPATAVDVSVAQV
                     SWYPRDSWVRYVSSGGAAGDGILFSGGALGVDSTASECPDRPSGSDAQLPYRVNFTPR
                     ASWYDPVSGTAGIYGGGAVTFRWQGHGIDLTAADPEVEINGAASRAIFRFQGSGSTPF
                     PNQRAALVSLDLTGKPTISGGGKTFTYDLVRGTLTPDGVSVFAGFYTPPDNDEFGCVS
                     VAFTTP"
     gene            4351..>4585
                     /locus_tag="VFB52_03200"
     CDS             4351..>4585
                     /locus_tag="VFB52_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZO05366.1"
                     /translation="MFQKVLIANRGEIAIRVARTLKEMGIISVAVYSEIDREAPHVRA
                     ADEAYLIGPAVPAESYLNIAKIIETAVEAGAEGI"
BASE COUNT          663 a         1578 c         1669 g          675 t
ORIGIN      
        1 cggcgccgtt ggggccgacc accgcgacca gctcgccttg gccgagggcg aggtcggcgc
       61 cggtgaggat ctcggtgtcg ccgatcttga cttcgacgcc cttggcgtcg agcagcagct
      121 ccgtgttcgg ggcggctgcc actagaccgc cttgcgcagc agccagagga agagcggcac
      181 cccgaggatc accatgaacg ggccgaccgg catttcgatc ggcgcccgca gggtccgcgc
      241 cagggtgtcg gcgccggcga gcagagcggc gccgccgatc gccgaggcgg gcacgacgaa
      301 ggtgtggctg gagctgccgc cggccatccg cacgacgtgg ggcacgacca ggccgaggaa
      361 gccgagcagc ccggcgatcg ccgccgaggc cgccgccagc agcgccgccg cgatcccggc
      421 ggcgagccgg atcagctgcg ggcgcgagcc gagcgaggca gcgacgtcgt cgccgagcgc
      481 gagccggtcg agcggccgga tcagcagcag ggcgaagatg aagccgacgg cgaagtaggg
      541 ccagatcgtc tgcagctcct cccagcccga ggaggtgagg ccgccggcga tgaagaagat
      601 cgccgagggg gcgcgctcgg ggtaggcggc gagcagaccg gtcgtgcccg cggtgaacag
      661 cgagagcatc gcgatcccgg cgaggatcac cttgccgacg ctgccgcggt tgcgccccga
      721 ccaggcgaag aggaagacca gggtcgccgc cagcaggccg aagaagaggg cgccgaccgg
      781 cagcagcgcg atcgagccgg ggaagaggat gatgatcagg atcgcgccga agccggcgcc
      841 gccggtcacc ccgatcacgt cgggcgaggc gagcgggttg ccgagcgccc cctggaggat
      901 cgcgccggcg atccccagcg ccgccccgac caccatcgcc tcgaccgtgc gcggcagccg
      961 cagcgcctcg acgatctgct gctcgggccc gcccggcccc ttgccgagca ggatgtcgat
     1021 cacgtcggag aacgggacct tgaccgcgcc cagcgccagc gacgccaccg tcgccagcgc
     1081 gagcgcgacc agcagcgccg cgaagaccga tacccgccgc gccaaggagc tcagttcttc
     1141 aggtaagcgc tgcgcacctt gcggatcgtg cgggcgatgt cggtgccggc ctgcagcagc
     1201 gagttgtcaa ccgagacgta gatccgaccg ttctgcgccg ccgaggtgct ctgccaggcc
     1261 gggttgtcct tcaggtattc ggtcaggctg gggatgtcgg aggcgttggc gtggggcacg
     1321 gcgatgatca cgtccgggtt ttcggcgacc acgagttcgt cggagatccg cgcgaagccg
     1381 gagtcggagt cgacgccgcc ggtcagcagt tcgccgccgg ccttgatcac gatttcgccg
     1441 ccccagctgt tcgacagcat cgtgaacggg gtgcggccga cgccgagcag cagcatcacc
     1501 ttcgggtggc tcttgatccc cttggtcgcc gcttcgacgc ttttcttcat cgctttcagc
     1561 tgcgcgcgcg cctggcgctg cttgccgacc agctgcccga tccggtaggt gtcgcggtag
     1621 gcgcggccga ttttcagcgg ctcgtagacg cggacctcga tcccgaggtc ttccatcgcc
     1681 tggttgcctt tcacccaggt gcgcgaagag agcaccacgt cggggcgcag cgaggcgagc
     1741 tgctcgaggt tcggcccgtt cgggtgcgag agcggcagca cggtgacgtt cttcagcttc
     1801 ggcgagagcc gggtctggcc gcccagcgtt tcgccgaccc cgaccggggt gacgccgagc
     1861 ttggcgaggg tgttggcggc gaacggggtc agcgcgacga tccgcttggg gacgtcgttg
     1921 cccgactggg cggcgccggt gacggcgggc agagcgaggg cgatcagcgc ggccgcgagg
     1981 gccgcaaggg tggcgagtcg cttcacgccg gcacccgcgc gtcgatcacg tcgtagttgc
     2041 ggacctcgct gctgtgggcg agctggccgt cggcgtgctg gtggctgagc ttgaacagct
     2101 cgctgttcag ccaaccctgg aaggcttcct cgctctccca gcggctgagc acgacgtact
     2161 cgccgtcgcg atcgcgccgc agcagctcga agccggcgaa accgggagct ttgtggacgt
     2221 ggctctcgcg ccccaggaac tgttgctcga acgcctcccg tttgtcctcg tccaccttca
     2281 cgacgttcat cacgatcttc aactgcccgg ctcctctcgc gctcgcgaac tctgcaattc
     2341 ctagttaggc gtacctaatt agggtagccg aaggaatttc gcggcgttgt ggcgcggctc
     2401 aggggcgcgg cggaacgacg ggctcgccga gcccgtaggt gtcgtagacg ccgaactcgg
     2461 cgaaggtctt gtgggtcggg ccgtggtagt cggaggaggc ggtggggtag aggccgagct
     2521 cctggcaaag gccgagcagg tgcgcggtct gctcgcgggt gtgcggcggg tagtaggtct
     2581 cgatcccgtc gggctgcagt gagcggatca ggtcctcgac ctcgcccggg tcggagatgt
     2641 cccagtaggg gtgggcgatc acggcgacgc cgccggcgcc gcggatcagc tcgatcgcct
     2701 ccgccgcgtc gggccagcgg cgcggcacga acgccttcgc gcccgggacc aggtactcct
     2761 cgaagaaggg gccgaggtcg ccgctcgcgc cggccgcctt ggcgatgtgg gggcggccga
     2821 tcgagtcggc gtcgccggcc tcgcggatcg cgtcctcgag ggtcaggtcg aagccctcgc
     2881 ggcgcaggtt ctcgacgatc tcgccggccc ggttgcggcg ctcctgctgg gcgcgggcgc
     2941 agatcggttc catcgtcggc aggtcgatcc agtagccgca gatgtgcagg tcttcggcgt
     3001 atgggtggac gcaggacatc tcgatcgccg ggaccagttc gatccccagc cgctccgccg
     3061 cctcggcggc cgcgggcacg ccggcgaccc cgtcgtggtc ggtcagcgcc agcgtggtga
     3121 cgccggcctc ggcggctttc tcgaccacct cggccggcgg cagctgcccg tcggagacgg
     3181 tcgagtgcga ttggagctcg atcagcacgg ccaccaccct aatcgcgcga tgaattaggg
     3241 cgccctaatt cgaccttgct atcgtggccg ccgtgttgaa gcgctccctc tttctcggcg
     3301 ccctgctggc gctgctgttg gcggtcccgg cccaggcgaa gctgctctac gtcggcgaca
     3361 agggcaccgc cccggtcggc aaggtctcct gcccggctgg ccagggctgc gcggtgcaga
     3421 cgccgaagcg ggtgaaggcg aagatcggga cgaagagctt ctgggcgagg gtgctggcgc
     3481 cgaagcgggt cgccgccggc aagcagggga cggtgcgggt caagttcggc ggcggggcgc
     3541 tcggcgacct cgccgggcgg acgacgacgg tcaaggtgaa ggcggtcgtg cgccaggacg
     3601 gcgtcgcccg cacccggctg ctgcaggtcc gcctgcgccg cgccgcgctc cccgccggcg
     3661 gctccgacaa cccgagctcc gggccgctcg gcaacgaacc gccgctgctc gtgcgcccgg
     3721 cgaccgcggt cgacgtcagc gtcgcccagg tcagctggta cccgcgcgac tcctgggttc
     3781 gttacgtctc cagcggcggc gccgccggcg acggcatcct cttttccggc ggcgccctcg
     3841 gcgtcgactc gaccgcctcc gaatgcccgg accggcccag cggtagcgac gcgcagctgc
     3901 cctaccgggt caatttcact ccgcgggcga gctggtatga cccggtcagc ggcaccgccg
     3961 ggatctacgg cggcggcgcg gtcaccttcc gctggcaggg ccacggcatc gacctgaccg
     4021 cggccgaccc ggaggtcgag atcaacggcg ccgcctcgcg cgcgatcttc cgcttccagg
     4081 gcagcggctc gacgcccttc ccgaaccagc gcgccgcgct cgtcagcctc gacctgaccg
     4141 gtaaaccgac gatctcgggc ggcggcaaga ccttcaccta cgacctcgtg cgcgggaccc
     4201 tgaccccgga cggcgtcagc gtcttcgccg gcttctacac gccgccggac aacgacgaat
     4261 tcggttgcgt ctcggtcgcc ttcacgactc cctagggtcg ctgcgagggc tgtcggcatt
     4321 cggaacgccg cataaataga ctgcgccgcc gtgttccaga aggtcttgat tgctaaccgc
     4381 ggcgagatcg cgatccgcgt cgcccgcacg ctgaaggaga tggggatcat ctcggtcgcc
     4441 gtctactcgg agatcgaccg cgaggcgccg cacgtgcgcg ctgccgacga ggcctacctg
     4501 atcggcccgg cggtcccggc cgagagctac ctgaacatcg ccaagatcat cgagaccgcg
     4561 gtggaggcgg gggccgaagg gatcc
//
LOCUS       DASRIG010000116         7205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1114845, whole genome shotgun sequence.
ACCESSION   DASRIG010000116 DASRIG010000000
VERSION     DASRIG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114845"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1269)
                     /gene="ispG"
                     /locus_tag="VFB52_03205"
                     /gene_synonym="gcpE"
     CDS             complement(13..1269)
                     /gene="ispG"
                     /locus_tag="VFB52_03205"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZO05367.1"
                     /translation="MASKRQIWVGKVPIGGGAPVAVQTMTKTETANLAATMEQIRKVA
                     DAGADIVRCAVPREQDAEALKTIVKESPIPVIADIHFNHTLALKAIDAGADCIRLNPG
                     NIGGREKVAEVAVKANAANVPMRIGVNSGSLPKHLHELESEAPVEALVAAAVEFVELM
                     EGLDFTNFKVSIKSTNVPNTIAANRLLSEKIPYPLHLGITEAGTKWSGSLKSAVGLGT
                     LLADGVGDTIRISLSTFHAEEEVKVAWEILKALQLREKGPVLIACPTCGRLQFDMDSV
                     VAEIEQRLESYEEPVEVAVLGCAVNGIGEAKAADFGIAGAKNEGLIFAHGKPLRKVPQ
                     AVLVDTLFEEIDKSLDRGGKVEFDEAESREGAEWVANIERENEGDLTPEKIAQMEHEA
                     AEKGESDKVLLDEAASPTAGRRFTRA"
     gene            complement(1596..2687)
                     /locus_tag="VFB52_03210"
     CDS             complement(1596..2687)
                     /locus_tag="VFB52_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZO05368.1"
                     /translation="MNWLLIFLGFAVLIILHEAGHFFAAKATGMRVEKFFLFFGPKLW
                     SVRRGETEYGVAAIPLGGYVKITGMNPEEEIPPEVEDRAYYRQPVWKRIVVIGAGPAV
                     NIVLAFAILFTVYWANGDQQLAQKVGTVIPDSPAAQTLQPGDRVLAVDGNAYPGVSTE
                     RRLERFGEAVASHECAGKQVDGCTAATPVALRIERDGEVQTILVKPEYDAAAKRSLVG
                     FRYGSNTIEVGPGEAADEAVTAVWKVATGTFEVFGRLFEQEQREKVSGIVGVSDVANQ
                     TLDESATNSLLLLALVSLSLGLINLLPILPLDGGHIFWSLVEKVRGRPVSLRVMEKAS
                     VIGFALVLMLFVLGLSNDLGRIGDGIQNR"
     gene            complement(2684..3829)
                     /gene="dxr"
                     /locus_tag="VFB52_03215"
     CDS             complement(2684..3829)
                     /gene="dxr"
                     /locus_tag="VFB52_03215"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZO05369.1"
                     /translation="MKKLLILGSTGSIGTQALEVVARSEELQVVGLAAGRDWEAVVEQ
                     AREHGVPTIALADPDAAERARGAWSGRVLAGEEGVRELAASSGADLVLNAIVGAAGLA
                     STVVALTEGIDVALANKESLVVGGELVMALAEATDSRLLPVDSEHSALHQLIADEAPG
                     TVEKLVLTASGGPFRGREDLTGVSIEDALAHPTWEMGGRITIDCATLMNKGFEAIEAH
                     HFFAVPYERIDVVVHPQSLIHSLIQLNDGSSLAHLGYPDMRVPISYALHYPERADVDL
                     EQLDLAKVGSLTFEQPDLETFACLRLALEAGEAGGTAPCVLNAADEVAVAAFLAGEIP
                     FTGIAAVIERVLGEMPARPVTHFEDLFETDAEARRRSEDHVRGLTPA"
     gene            complement(3870..5747)
                     /locus_tag="VFB52_03220"
     CDS             complement(3870..5747)
                     /locus_tag="VFB52_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05370.1"
                     /translation="MGERSSIHRGWVALACALAALFCLPAGASAEGWSFKFATPEKVE
                     GTETRVGVDGDGNVTTIWRNDTNITDALGTLTFSSAVLPFGADKYDGPLPISSKGGGS
                     PVIGVLANGNAVVAWERNNGGAPSKDIIEGATRTLGVWTGVTELTDSGDETADYLTMD
                     MLPSGEGLLAWRVGGTEKDKVRLIQGGNFQVGVAKTFNTSPYSSNNEADVAIAPDASR
                     RFVAGHKNDLGNTPLNLFTYEGSSWDSGTFISKPSLVPQVAVAPNGEPVTAWLDSNVL
                     KLKRGGSDIVTVANVGNLSRLDLAVGPNTAEFPNGMVLVTWRQFVQDPEELAGACCYQ
                     ARAAVGNGVTMGAPIELSDELESVVNAPAVQAAIAPDGTAYTAWTRFDGGFWIPEASI
                     RPPGESFPSVADQISGGDAYVQDLVVDAEGRAIVGIDHIDSEGDELYFRAGTSIYTPG
                     PSEPKEEPGPPPAGPPAPPPADNTPPKVKIGLSRNVFAPGGKLNQLAVETGDPSYVWK
                     KVRTPLKFGTRVLVNLNEAANLTIKVNKLGCLTAKPGNPTRNLSGQCVKRDDDVQRLR
                     VKGKAGANKLTYLGDWGGGKVKPGALYQFVVVATDQFGNASKPRAVGFSLDGKQNANG
                     F"
     gene            5848..6858
                     /locus_tag="VFB52_03225"
     CDS             5848..6858
                     /locus_tag="VFB52_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO05371.1"
                     /translation="MPPTSAADLRAALDFVAEAHSFESLEAFRHGILPGLQELVPSDL
                     VGYNEVDPGGEALVLTYPWQVPVQVNSELPRLAHQHPLISVQANGDNATYKISDFLST
                     REFRSLELYAGLYRQIGAEDQIAFGLNGPVVIGIAMNRDRRSFSERDREMLELLRPHL
                     GQAFERVRERERSASLLAALERGLAERGSAIVLVERNGNVAHLTGAALELLGRYFPER
                     RGASLPDPIAAWLAAGAFEPLVVTTDAGTLTLRAQPEGATTVLALDEVAAVTPERLLP
                     LGLTRRQAEVLALLANGAGVERIARDLYVSPATVRKHLEHIYVRLGVHTRAEAIERAR
                     GV"
     gene            complement(6855..>7205)
                     /locus_tag="VFB52_03230"
     CDS             complement(6855..>7205)
                     /locus_tag="VFB52_03230"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZO05372.1"
                     /translation="AAYATGRMFGSHPIAPTISPNKTVEGLIGGFVIGTMGFWFAGLY
                     QDWLSGTDALIIGAAVAAIAPIGDLFESMLKRDLGTKDTGTLFGPHGGILDRLDAVFF
                     TVVVGYYLSVWLVY"
BASE COUNT         1034 a         2525 c         2420 g         1226 t
ORIGIN      
        1 cttgggttcg gctcaggcgc gggtgaagcg gcggccggcg gtcggcgacg cggcctcgtc
       61 gagcaggacc ttgtcggact cgcccttttc ggccgcttcg tgctccatct gggcgatctt
      121 ctcgggggtc aggtcgccct cgttttcgcg ctcgatgttg gccacccact cggcgccctc
      181 gcgcgactcc gcctcgtcga actcgacctt gccaccgcgg tcgagcgact tgtcgatctc
      241 ttcgaagagg gtgtcgacga gcaccgcctg cggcaccttg cgcagcggtt tgccgtgggc
      301 gaagatcagg ccctcgttct ttgctccggc gatcccgaag tcggcggctt tggcctcgcc
      361 gatcccgttg acggcgcagc cgagcaccgc gacctcgacc ggctcctcgt agctttccag
      421 ccgctgctcg atttcggcga cgaccgagtc catgtcgaac tgcagccgcc cgcaggtcgg
      481 gcaggcgatc agcaccggcc ctttctcgcg cagttggagc gccttgagga tctcccaggc
      541 gactttgacc tcctcctcgg cgtggaaggt agagaggctg atccggatcg tgtcccccac
      601 cccgtcggcg agcagggtgc cgaggccgac cgccgatttc agcgagcccg accatttcgt
      661 ccccgcctcg gtgatcccga ggtgcagcgg gtaggggatc ttctcgctca gcagccggtt
      721 ggcggcgatc gtgttcggca cgttggtcga cttgatcgag accttgaagt tggtgaagtc
      781 gagcccctcc atcagttcga cgaactcgac cgcggccgct accagcgcct cgaccggcgc
      841 ttcgctctcc agctcgtgca ggtgtttggg cagcgagccg gagttgaccc cgatccgcat
      901 cggcacgttg gcggcgttcg ccttcaccgc gacctccgcg actttctcgc ggccgccgat
      961 attgcccggg ttgagccgga tgcagtcggc gccggcgtcg atcgctttca gcgccagcgt
     1021 gtggttgaag tggatgtcgg cgatcaccgg gatcggcgat tccttgacga tcgtcttcag
     1081 cgcctccgcg tcctgctcac gcggcaccgc gcagcggacg atgtccgcgc cggcgtcggc
     1141 gaccttgcgg atctgctcca tcgtcgccgc caggttcgcg gtctcggtct tggtcatcgt
     1201 ctgcacggcg accggagcgc cgccgccgat cggcaccttg ccgacccata tctgccgttt
     1261 ggaagccatg cagacagcgt atccagccca cttcccgggc ggtttcgggg tggttaccct
     1321 gggcgcgaca tggcgacccg tactgaggcc ggccgggcct gtcgagtctt gcgcgctata
     1381 ggccgcataa gtcgacaccg ccgcttcgag ccccggtgcg cgccgccagc cagacggaac
     1441 tcccgggatg gcccgtgtgg aggggtcggg tgactctcgg aacgtcttgt actttttccc
     1501 gcccccaatc tcggacggct ctatcccgac ctcgaaaaag ttaagtgcag agagtcaccc
     1561 gacccctccc cgcatgccct cccgaagtac gcccgtcagc ggttctgtat gccgtcgccg
     1621 atccggccca ggtcgttgga taacccgagc acgaacagca tcagcactag ggcgaagccg
     1681 atcacgctgg ctttttccat cacccgcagc gagaccgggc ggccgcggac cttctcgacc
     1741 aggctccaga agatgtggcc gccgtcgagc ggcaggatcg gcagcaggtt gatcaggccg
     1801 agcgagaggc tgaccagagc gagcaggagc agggagttcg tggcgctttc gtcgagcgtc
     1861 tggttggcga cgtcgctgac cccgacgatc cccgagactt tttcgcgctg ctcctgttcg
     1921 aacaggcggc cgaagacttc gaaggtcccg gtggcgactt tccagaccgc ggtgacggct
     1981 tcgtcagcgg cttcgccggg accgacctcg atcgtgttgc tgccgtagcg gaagccgacg
     2041 agcgagcgtt tggccgccgc gtcgtactcg ggcttgacca ggatcgtctg cacctcgccg
     2101 tcgcgttcga tccgcagcgc cacaggggtc gcggccgtgc agccgtcaac ctgcttgccg
     2161 gcgcattcgt gcgaggcgac cgcttcgccg aaccgttcca ggcggcgttc ggtgctgacg
     2221 cccggatagg cgttgccgtc gacggcgagc acacgatcgc ccggctgcag ggtctgggcg
     2281 gccggtgagt cggggatcac ggttccgacc ttctgcgcca gctgctggtc gccgttggcc
     2341 cagtagaccg tgaacagaat cgcgaaggcg agcacgatgt tcaccgccgg gccggcgccg
     2401 atcacgacga tccgcttcca gaccggctgg cggtagtagg cgcggtcctc gacctccgga
     2461 ggaatctcct cctccgggtt catcccggtg attttcacgt agccgccgag cgggatcgcg
     2521 gcgaccccgt actcggtctc cccacgcctg accgaccaca gtttcggccc gaagaagagg
     2581 aagaacttct ccacccgcat cccggtcgcc ttcgcggcga agaagtggcc cgcctcgtgc
     2641 aggatgatca gcacggcgaa gccgaggaag atcagcaacc agttcacgct ggagtcaacc
     2701 cccgaacgtg gtcttcgctg cggcgccgcg cctcggcgtc ggtctcgaag aggtcctcga
     2761 agtgggtgac cgggcgcgcc ggcatctcgc cgagcacccg ctcgatcacc gcggcgatcc
     2821 ccgtgaacgg gatctcaccg gcgaggaagg cggcgaccgc gacctcgtcg gcggcgttca
     2881 gcacacaggg cgccgtcccc cccgcctcgc ccgcctccag ggccaggcgc aggcaggcga
     2941 aggtctccag atcgggctgc tcgaaggtca gtgagccgac cttggcgagg tcgagctgct
     3001 cgaggtcgac gtcggcgcgc tcggggtagt gcagcgcgta ggagatcggc acccgcatgt
     3061 ccgggtagcc gaggtgggcc agcgaggagc cgtcgttgag ctggatcagc gagtggatca
     3121 gcgattgcgg gtgcacgacc acgtcgatgc gctcgtaggg caccgcgaag aagtggtgtg
     3181 cctcgatcgc ctcgaagccc ttgttcatca gcgtcgcgca gtcgatcgtg atccgcccgc
     3241 ccatctccca ggtcgggtgc gcgagcgcgt cctcgatgct gaccccggtg aggtcctcgc
     3301 ggccgcggaa cggcccgccg gaagcggtga gcacgagctt ctcgaccgtg cccggcgcct
     3361 cgtcggcgat cagctggtgc agcgccgagt gctcggaatc gaccggcagc aggcgggagt
     3421 cggtcgcctc ggcaagggcc atcaccagct cgccaccgac caccaggctc tccttgttgg
     3481 cgagcgcgac gtcgatcccc tcggtcagcg cgaccacggt cgaggcgagc ccggccgcac
     3541 cgacgatcgc gttcaggacc aggtcggcgc ccgagctcgc ggccagctcg cgcacgccct
     3601 cctcgccggc gagcacgcgg ccgctccagg cgccgcgggc gcgctcggcg gcgtcggggt
     3661 cggcgagcgc gatcgtcggc acgccgtgct cgcgcgcctg ctcgaccacg gcctcccagt
     3721 cccgcccggc ggcgaggcca acgacctgga gctcttcgga acgcgcgacg acttccagcg
     3781 cctgcgtccc gatcgacccg gtcgacccga ggatcagcag cttcttcatg ccccgcagag
     3841 gatacggagc cgaccttacg ggtccctaat cagaagccgt tggcgttctg cttgccgtcg
     3901 aggctgaagc cgacggcgcg tggtttcgag gcgttcccga actggtcggt cgcgactacg
     3961 acgaactggt agagcgcccc cggtttcacc ttgccgccgc cccagtcgcc gaggtaggtc
     4021 aacttgttgg cgcccgcttt gcctttgacc cgcagccgct gcacgtcgtc gtcgcgcttc
     4081 acgcattgcc cggagaggtt ccgcgtcggg ttccccggtt tggcggtcag gcagccgagc
     4141 ttgttgactt tgatcgtcag gttcgccgcc tcgttgagat tgaccagcac gcgggtgccg
     4201 aacttgagcg gcgtgcgcac tttcttccac acgtaggagg ggtcgccggt ctcgaccgcg
     4261 agctggttca gcttgccgcc gggcgcgaac acgttgcgcg agagtccgat cttcaccttc
     4321 ggcggcgtgt tgtcggccgg cggcggagcc ggcgggcccg cgggcggagg accgggttct
     4381 tctttcggct ccgacggtcc cggcgtgtag atcgaagtcc cggcgcggaa gtagagctcg
     4441 tcgccctcgc tgtcgatgtg gtcgatgccg acgatcgccc gcccttcggc atcgacgacg
     4501 aggtcctgca cgtaggcgtc gccgccggag atctgatcgg cgaccgaggg gaagctctcg
     4561 cccggcggcc ggatcgaggc ttccgggatc cagaagccgc cgtcgaagcg ggtccaggcc
     4621 gtatacgcgg tcccgtccgg ggcgatcgcg gcctgcacgg ccggcgcgtt gacgacgctc
     4681 tcgagctcgt cggagagctc gatcggcgcg cccatcgtca ccccattgcc gaccgcggcc
     4741 cgcgcctggt agcagcaggc ccccgccagc tcttcggggt cctggacgaa ctggcgccag
     4801 gtgaccagga ccatgccgtt cgggaactct gcggtgttcg ggccgaccgc gaggtcgagc
     4861 cgcgagaggt tgccgacgtt ggcgacggtg acgatgtccg agccgccccg tttcagcttc
     4921 agcacgttcg agtccagcca ggcggtgacc ggttccccgt tcggcgccac cgcgacctgc
     4981 ggcaccaggc tcggtttcga gatgaaggtg ccgctgtccc agctcgagcc ttcgtaggtg
     5041 aagaggttga gaggggtgtt gccgaggtcg tttttgtggc cggcgacgaa gcggcggctg
     5101 gcgtccgggg cgatcgccac gtcggcttcg ttgttgctcg agtagggcga ggtgttgaag
     5161 gttttggcga cgccaacctg gaagttgccg ccctggatca gccgcacttt gtccttttcc
     5221 gtgccgccga cccgccaggc aagcagcccc tcgccgcttg gcagcatgtc catcgtcagg
     5281 tagtccgcgg tttcgtcgcc gctgtcggtc agttcggtga cgccggtcca aacgccgagc
     5341 gtgcgggtcg cgccctcgat gatgtctttg ctcggagcgc cgccgttgtt gcgttcccag
     5401 gcgacgaccg cgttgccgtt ggcgaggacc ccgatcaccg ggctgccgcc gcctttcgag
     5461 ctgatcggca gcggcccgtc gtatttgtcg gcgccgaagg gaagcaccgc ggaggagaac
     5521 gtgagggtgc cgagggcgtc agtgatgttg gtgtcgttgc gccagatcgt ggtcacgttg
     5581 ccgtcgccat cgaccccgac ccgcgtttcc gtcccctcga ctttctccgg cgtggcgaac
     5641 ttgaagctcc agccctcggc ggaagcgccg gcgggcaggc agaagagggc cgccagcgcg
     5701 caggccagcg caacccaacc acgatgaatc gacgagcgtt cccccatctg tcgccccact
     5761 ctatttggta cgggcccgtc ccggaatacg tcaaatgccg tatttcgagt atttgcagcg
     5821 ccctttagcc ctcctacact ctgcccgatg ccaccgacgt ccgccgcgga cctgcgcgct
     5881 gccctcgact tcgttgccga ggcgcactcg ttcgagagcc tcgaagcctt ccgccacggc
     5941 atcctccccg gcctgcagga gctcgtcccc tccgacctgg tcggctacaa cgaggtcgac
     6001 ccgggcggcg aggcgctggt tctgacctac ccctggcagg tgcccgtgca ggtcaactcc
     6061 gaactacccc gcctcgccca ccagcacccg ctgatctcgg tccaggcgaa cggcgacaac
     6121 gccacctaca agatctccga cttcctcagc acccgcgagt tccgctccct ggagctctac
     6181 gcgggcctct acaggcagat cggcgccgag gaccagatcg cctttggcct caacgggccg
     6241 gtcgtgatcg ggatcgcgat gaaccgcgac cgccgcagct tctccgagcg cgaccgggag
     6301 atgctcgagt tgctgcgccc gcacctcggg caggccttcg agcgcgtccg cgagcgcgag
     6361 cgctccgcct cgctgctcgc cgcgctggag cgtggcctcg ccgagcgcgg cagcgcgatc
     6421 gtcctggtcg agcgcaacgg caacgtcgcc cacctgaccg gggcggcgct ggagctgctc
     6481 ggtcgctact tccccgagcg ccgcggcgcc tccctgcccg acccgatcgc cgcctggctc
     6541 gcagccggtg ccttcgaacc gctggtcgtc acgacggacg ccggcacgct gaccctcagg
     6601 gcccagccgg agggcgccac caccgtgctc gcgctcgacg aggtggcggc cgtcaccccc
     6661 gaacgcctcc tccccctcgg cctcacccgc cgtcaggccg aggtcctcgc cctgctcgcc
     6721 aacggagccg gcgtcgagcg catcgcccgc gatctctacg tcagccccgc caccgtgcgc
     6781 aagcacctcg agcacatcta cgtgcgcctc ggcgtgcaca cgcgggccga agcgatcgag
     6841 cgcgcccggg gcgtctagta gacgagccag accgagaggt agtagccgac gacgaccgtg
     6901 aagaagacgg cgtcgaggcg gtcgaggatg ccgccgtggg ggccgaagag ggtgccggtg
     6961 tccttggtcc cgaggtcgcg cttcagcatc gactcgaaga ggtcgccgat cggggcgatc
     7021 gcggcgacgg cggcgccgat gatcagggcg tcggttcccg agagccagtc ctggtagagg
     7081 ccggcgaacc agaagcccat ggtgccgatc acgaagccgc cgatcaggcc ctcgacggtc
     7141 ttgttcgggg agatcgtcgg ggcgatcggg tggctgccga acatccggcc ggtggcgtag
     7201 gcggc
//
LOCUS       DASRIG010000117         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_829520, whole genome shotgun sequence.
ACCESSION   DASRIG010000117 DASRIG010000000
VERSION     DASRIG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829520"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2199
                     /gene="ligA"
                     /locus_tag="VFB52_03235"
     CDS             169..2199
                     /gene="ligA"
                     /locus_tag="VFB52_03235"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZO05373.1"
                     /translation="MSTTAEDRAAELRRTLDHHNHRYYVLDDPEVSDSDYDALLNELR
                     DIEAEHPELRTPDSPTQRVGAPPLERFEPAVHHEAMLSLGNARNEEELRAWETRLANY
                     LKRLDITASQFSYTTEPKIDGLAITLTYEDGVLVRGATRGDGQTGEDVTQNLRTIGSV
                     PLSIPDAPELIEVRGEAYLPIAAFKALNERRAENEEPTFANPRNSAAGSIRQLDPALA
                     AERPLSTWIYGIGAVRGLDLATHMDEIEWLDKRGFKVNPETRRHEGVEGVVKRCHWWE
                     EQRDQLDYEIDGVVVKVDERALWRELGVVGREPRWAIAWKFPPTTATTKMVKVVWNVG
                     RTGHLVPFAMLEPVHVGGVTVTTATLHNEEDLERKDVREGDEVVVMRAGDVIPQVVSP
                     KLPRQNKSARKPKPPKKCPECDTPTVKPEGAVFTICPNRGGCPGQASQHVKHFVSKGA
                     MDIEGLGEEVAFNMLRQGVIEDVADIYDLREETLVGSKVGKKSFKEKSAAALVAAIEV
                     SKKQPFKRVLYALGLPGVGYVTAEALADHFGSIDALHQADPEQIEEVEGVGPIMAVQI
                     AESLADEPTWALIEKLRERGLRLEADESEKRATGGPLEGKTVVLTGTLPVLTREEAGA
                     LVKAAGGKVTSSVSKKTDYVVAGDNPGSKLAKAEKFGTEVLDEAGLRELVGA"
     gene            complement(2213..>3425)
                     /gene="dacB"
                     /locus_tag="VFB52_03240"
     CDS             complement(2213..>3425)
                     /gene="dacB"
                     /locus_tag="VFB52_03240"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZO05374.1"
                     /translation="AVCALALLAAASAQAGPACNQMKRWLKSGSGVSGLLVVDAESGK
                     TLCASGAGRPLPLASNMKMFTTATALSKLGPEHRIATKVFRDGKVDSRGVLHGSLYLQ
                     GGGDPALGTPAFDAGYLGGLGTNILLLRPQIRAAGIKSITGRLYADDSIFDRLRGVAD
                     SGYATSYYIGPLSGLSFNAGFAGNSSSSGFSSDPAKSAARKLVTSLRNGGVTMPGAIA
                     LGVTPPNADRVAMVESAPLTELVDLTNIYSNNFFAETLVKLLGAEFGNAGTTAAGTAV
                     VEGFAQSLGSSVHAVDGSGLTRSNRASPRQVINLLLGMRKQEVGDVFIQDLPLTGAEG
                     TVDDRMRGNAANRRCRVKTGTLTGVSNLSGYCFNKSGKVMAFSILMGSVGNLSLAHQR
                     QDQIAGTVASF"
BASE COUNT          581 a         1135 c         1241 g          468 t
ORIGIN      
        1 cggcaacatg ccgagccacg agcaggagga cggcacggac ttcgcggcca tctcggacgg
       61 ctgcatctcg gtcacgcccc tgcacttcga cctcaacgac ctggccggcg tcgagcagct
      121 gaacggcttc gacctggccg ccctgctcga gccggcggcg cgggaagtgt gagtacgacc
      181 gccgaggaca gggcggccga gctgcggcgg acgctcgacc accacaacca ccgctactac
      241 gtcctcgacg accccgaggt gtcggactcc gactacgacg cgctgctgaa cgagctgcgc
      301 gacatcgagg ccgagcaccc ggagctgcgc acgcccgact cgccgaccca gcgggtcggg
      361 gcgccgccgc tggagcgctt cgagccggcc gtgcaccacg aggcgatgct ctcgctcggc
      421 aacgcccgca acgaggagga gctgcgcgcc tgggagaccc ggctcgccaa ctacctgaaa
      481 cggctcgaca tcaccgcttc gcagttcagc tacacgaccg agccgaagat cgacgggctc
      541 gcgatcacgc tcacctacga ggacggggtg ctggtccgcg gcgccacccg cggcgacggc
      601 cagaccggcg aggacgtgac ccagaacctg cgcacgatcg gctcggtgcc gctgtcgatc
      661 ccggacgcgc cggagctgat cgaagtgcgc ggcgaggcct acctgccgat cgccgccttc
      721 aaagcgttga acgagcggcg ggccgaaaac gaggaaccga ccttcgccaa cccgcgcaac
      781 tcggccgccg gctcgatccg ccagctcgac cccgcgctgg ccgccgaacg gccgctctcg
      841 acctggatct acgggatcgg cgcggtccgc gggctcgacc tcgccaccca catggacgag
      901 atcgagtggc tcgacaagcg cggcttcaag gtcaacccgg agacccgccg tcacgagggc
      961 gtcgagggcg tggtgaagcg ctgccactgg tgggaggagc agcgcgatca gctcgactac
     1021 gagatcgacg gcgtcgtcgt caaggtcgac gagcgggcac tgtggcgcga gctcggcgtg
     1081 gtcgggcgcg agccgcgctg ggcgatcgcc tggaagttcc cgccgaccac cgcaaccacc
     1141 aagatggtga aggtcgtctg gaacgtcggc cgcaccggcc acctggttcc gttcgcgatg
     1201 ttggagccgg tccacgtcgg cggcgtcacc gtgaccaccg cgaccctgca caacgaggag
     1261 gacctcgaac gcaaggacgt gcgcgagggc gacgaggtcg tggtgatgcg ggccggcgac
     1321 gtgatcccgc aggtggtctc gccgaaactg ccgcgccaga acaagagcgc gcgcaagccg
     1381 aagccgccga agaagtgccc ggaatgcgac acgccgacgg tgaagccgga gggcgcggtt
     1441 ttcacgatct gcccgaaccg cggggggtgt ccgggtcagg cgtctcagca cgtcaagcac
     1501 ttcgtcagca aaggcgcgat ggacatcgag gggcttggcg aagaggtcgc cttcaacatg
     1561 ctcaggcaag gcgtgatcga agacgtcgcc gacatctacg accttcgcga ggagacgctc
     1621 gtcggatcca aagtcggcaa gaagagcttc aaagagaaat ccgcggctgc cctggtcgcg
     1681 gcgatcgagg tctctaagaa gcagcccttc aagcgtgtgc tctacgcgct ggggctgccc
     1741 ggcgtcggct acgtcacggc agaggccctg gctgaccact tcggctcgat cgacgccctg
     1801 caccaggccg accccgagca gatcgaggag gtcgagggag tggggccgat catggccgtg
     1861 cagatcgccg agtcgctggc cgacgagccg acctgggcgc tgatcgagaa gctgcgcgag
     1921 aggggcctgc ggctggaggc cgacgagtcc gaaaaacgcg ccaccggcgg cccgctcgaa
     1981 ggcaagacgg tggtcctcac cggcaccctg ccggtgctga cccgcgagga ggccggggca
     2041 ctggtgaagg ccgcgggcgg caaagtgacc agctcggtct cgaaaaaaac cgactacgtc
     2101 gtggccggcg acaaccccgg ctcaaagctc gcgaaagcgg agaaattcgg caccgaggtg
     2161 ctcgacgaag ctgggttgcg agagctggtc ggcgcctagc cgacctcgct ggctagaagc
     2221 tggcgacggt gccggcgatc tggtcctggc gctggtgggc gaggctgagg ttgccgacgc
     2281 tgcccatcag gatcgagaag gccatcacct tgccgctctt gttgaagcag tagccggaga
     2341 ggttgctgac cccggtcaag gtgccggtct tgacccggca gcggcggttg gcggcgttgc
     2401 cgcgcatgcg gtcgtcgacc gtgccctcgg cgccggtcag cggcaggtcc tggatgaaga
     2461 cgtcgccgac ctcctgtttg cgcatcccca gcagcaggtt gatcacctgg cgcggcgagg
     2521 cgcggttgga gcgggtcagg ccggagccgt cgacggcgtg cacggatgag ccgagcgact
     2581 gggcgaagcc ctcgaccacc gcggtcccgg cggcggtcgt gccggcgttg ccgaactcgg
     2641 cgccgagcag cttgaccagg gtctcggcga agaagttgtt cgagtagata ttggtcaggt
     2701 cgaccagctc ggtcagcggg gccgattcga ccatcgctac ccggtcggcg ttcggcgggg
     2761 tgaccccgag cgcgatcgcg cccggcatcg tgaccccgcc gttgcgcagc gaggtcacca
     2821 gcttgcgcgc cgccgacttg gccgggtcgg aggagaagcc gctggagctg ctgttgccgg
     2881 cgaagccggc gttgaaagag agcccggaga gcgggccgat gtaatagctg gtcgcgtagc
     2941 ccgagtcggc gacgccgcgc aggcggtcga agatcgagtc gtcggcgtag aggcggccgg
     3001 tgatcgactt gattccagcg gcgcggatct ggggccgcag caagaggatg ttcgtgccga
     3061 gcccgcccag gtagccggcg tcgaaggcgg gcgtgccgag cgccgggtcg ccgccgccct
     3121 gcaggtagag gctgccgtgg aggaccccgc ggctgtcgac cttgccgtcg cggaagacct
     3181 tggtcgcgat tcggtgttcg ggcccgagct tgctcagcgc ggtcgcggtg gtgaacatct
     3241 tcatgttcga ggccaggggg agcggccggc cggcgccgct ggcgcagagg gtcttgccgc
     3301 tttcggcgtc gaccacgagc aggccgctga ccccgctgcc gctcttcagc cagcgcttca
     3361 tctggttgca ggcggggccc gcctgggcgc tcgcggcggc cagcaacgcc agcgcgcaga
     3421 ccgcg
//
LOCUS       DASRIG010000118         5609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_830250, whole genome shotgun sequence.
ACCESSION   DASRIG010000118 DASRIG010000000
VERSION     DASRIG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830250"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VFB52_03245"
     CDS             complement(<1..1493)
                     /locus_tag="VFB52_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05375.1"
                     /translation="MPKLATPLFCALLAALALAASAGTATASPLKLDRSFGKAGLVQP
                     DFGPAYLPISYDAVQAEPSGAVVVARALGGSGPVWRRYDATGVLDPSFDPNSGAYPPI
                     EAVTAEGKTLRPDGGLILRYNADGTWDKTFGVDPEPGHRPFSEAVPFLIRALVSLPSG
                     KIAAAGWDIKHDRRRREIRQILVARFDSQGHLDPGFGGDGVVNLASDAGFAVKRLAGL
                     AAHGDEGVVVAADERLRRSYGITNPFHGNSLVLALGADGRPDPAFGKEGAVRSRSSIE
                     EFQVLPGGNLLVAGDRWGAELHVNSARLSDLYAMRLTPAGQPDPGFDGDGLAVADFGG
                     VDRFGSLQVEADGGLLLGGSSTGIGDRICVRFPGYCTETPVLARLLADGTPDPGFGDG
                     GRVRLKALSEPFASLGAGGGVEALAPLPGGEVAAGGSSGAVAFLAKIDRAGALVGGFG
                     SGGVVTERDPHHNETTAHAVAVDARGRILVAGSTSAGTFYASPENGVV"
     gene            complement(1612..2145)
                     /locus_tag="VFB52_03250"
     CDS             complement(1612..2145)
                     /locus_tag="VFB52_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HZO05376.1"
                     /translation="MFFLVILFIVVPIAELYVIIKVGEAIGILPTLALLFLDAILGSL
                     LLRHQGRGAWRRFNEALAQGRFPGTEVVDGLLIVIGGTLLLAPVFITDIVGLLFLIPP
                     TRAIVRAILKRFTIGRVAIFGTFGGGRGDQGGPGPFGPGGPGGQGPSRDYDYDVTAEE
                     VPTNGSGDGRQLPRTDD"
     gene            complement(2220..3635)
                     /locus_tag="VFB52_03255"
     CDS             complement(2220..3635)
                     /locus_tag="VFB52_03255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZO05377.1"
                     /translation="MSTEGRHLDRLTAVDASFLTNESSASHMHVGAILIFEGPPPKYV
                     DLVEHVRGRLALVPRFRQKLVVPPLEAGRPLWADDVNFNLTYHIRHTALPDPGGEAQL
                     KRLAGRVFSQQLDRSKPLWELWLAQGLERDRFAILTKTHHAMVDGVSGVDIGTVLFDL
                     EPVPDPVEIADEWVPQPEPGTTELVARGVSDFAAVPVKFAERAVSAVKKPERAARKAV
                     EALEGISEIVSAFADPAPDVPLNQKIGPHRRYVWARSELATFKKIKDSLGGTVNDVVL
                     AVVTGAVREWLHGRSIRTEGLELRALVPVSIRGEDERGNLGNRIALMRGPLPVYVEDP
                     VRRLRTITQAMEGLKRSKQALGAEVISRFNDFAPPTLLAQASRINFSTRLFNMIVTNV
                     PGPQLPLYVLGRELEEVFPIAFLPENHTLAVAIMSYNGKVGFGLLADYDSMEDIELLA
                     ESLNASLAELEAAASESAAAA"
     gene            3715..4683
                     /locus_tag="VFB52_03260"
     CDS             3715..4683
                     /locus_tag="VFB52_03260"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZO05378.1"
                     /translation="MPEATIAAPKRVLLAAPRGYCAGVDRAVQTVERALELHGAPVYV
                     RKEIVHNKHVVEELAKRGAIFVEEETEVPEGAIVVFSAHGVAPSVHENAASRGLQTID
                     ATCPLVTKVHVSARRFAAQGYTIVMIGHDGHEEVEGTTGEAPDSIVLIETADEVASLE
                     VPDPEKVAFITQTTLSVDETAEVIAALRAKFPSIVSSKSDDICYATTNRQIAVKQLAR
                     ECDLVLVIGSTNSSNSNRLVEVAREHGAGSHLIDNHTQVQEEWLAGVETVGITSGASA
                     PEELVESLVSFFRERGADDVSELRTVHEDVRFMLPKEIREELAARA"
     gene            4658..5563
                     /locus_tag="VFB52_03265"
     CDS             4658..5563
                     /locus_tag="VFB52_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05379.1"
                     /translation="MRSSRRGPRARRRRWGAPGCLAAALAAAALLCAPALAADAGPLP
                     FCGPDEAEEREARLAAMPGARPAPEGPLPFGPRNFNIHKADRTAVVLEGSRIGYRFGA
                     KNGYSRELVLNWRLGATLRRVDSAGRVKRALGRRHVGVGKVDDLEQMDLLFPAEHPGL
                     YRIDTRIRDLSSGKRVSYREYFEVLKRSVDVSIAVSVPSARPGEAVYGRVVNRGGTNL
                     TGVPAFLDVEREQGSTWVPVEQPPTPASVLDFHWWMHSGEATYCSRFDVPSDATPGRY
                     RFTASIYVVNLQRRETFTGGFEVAP"
BASE COUNT          841 a         2016 c         1955 g          797 t
ORIGIN      
        1 accaccccgt tctcggggct ggcgtagaac gtgcccgcgc tcgtgctgcc cgcgacgagg
       61 atccggccgc gggcgtcgac cgcgaccgcg tgcgccgtgg tttcgttgtg gtgggggtct
      121 cgctccgtca ccaccccgcc gctaccgaag ccgccgacga gcgctcccgc ccggtcgatc
      181 ttcgcgagga aggcgacggc ccccgagctg ccgcccgccg ccacctcgcc tccgggcagg
      241 ggggcgaggg cttcgacgcc gccgccggca cccagcgagg cgaacggttc tgagagcgcc
      301 ttcagccgca cccgcccgcc gtccccgaag cccgggtcgg gcgtcccgtc ggcgagcaga
      361 cgggcgagca ccggcgtttc cgtgcagtac cccgggaagc ggacgcagat gcggtcgccg
      421 atccccgtgc tggagccgcc gagcagcagc cctccgtccg cttccacctg cagcgaaccg
      481 aaccggtcga ccccgccgaa gtcggcgacc gcgaggccgt cgccgtcgaa accggggtcg
      541 ggctggcctg ccggggtcag ccgcatcgcg tagaggtcgc tgagccgggc gctattgacg
      601 tgcagctccg ccccccagcg gtcgcccgcg accagcaggt tgccgcccgg cagcacctgg
      661 aactcctcga tcgaggagcg cgagcggacg gcgccctctt tcccgaaagc cgggtcgggc
      721 cggccgtcgg ctcccagtgc aaggaccaag gagttcccgt ggaacgggtt cgtgatcccg
      781 taggagcgcc gcagtcgctc gtcggccgcg acgaccacgc cttcgtcgcc gtgcgccgcc
      841 aggccggcca ggcgcttgac ggcgaaaccg gcatcggagg ccaggttgac gaccccgtcg
      901 cccccgaatc ccgggtcgag atggccctgt gagtcgaagc gggcgacgag gatctgccgg
      961 atttcgcgtc gcctgcggtc gtgcttgatg tcccagccgg cggcggcgat cttgcccgac
     1021 ggcagcgaaa ccagtgccct gatcaagaac ggcacggcct cgctgaacgg tctgtgtcct
     1081 ggctccggat cgaccccgaa ggtcttgtcc catgtgccgt cggcgttgta gcgcaggatg
     1141 agcccgccgt ccggtcgcag ggtctttccc tccgcggtga cggcttcgat cggcgggtac
     1201 gcgccggagt tgggatcgaa ggacggatcg agcacgccgg tcgcgtcgta gcggcgccag
     1261 acggggccgc tgccacccag cgcgcgcgcg acgaccaccg ctccgctcgg ttcggcctgc
     1321 accgcgtcat agctgatcgg gaggtaggcg gggccgaagt cgggctggac caggccggcc
     1381 ttcccgaacg agcggtcgag ctttagcggg gacgccgtcg ccgtaccggc ggatgcggcc
     1441 agggcgagcg cggcgagcag ggcgcagaaa agcggagtgg cgagcttggg catcggtctc
     1501 acgggttcga gggacaggag gcgcgagcaa tcctcttctc cctcgccgtg cccgcaaagg
     1561 acagcggcgc cccgcatcgt gccaactctg gcccggggcg ggccagggtg ctcagtcgtc
     1621 cgtccgcggc agttgacgac cgtcgccact gccgttcgtc ggcacctctt cggcggtgac
     1681 gtcgtagtcg tagtcgcggc tcggcccttg gccgccggga ccaccgggtc cgaacggccc
     1741 cgggccaccc tggtcgcccc gaccaccccc gaacgtgccg aagatcgcta cccgcccgat
     1801 cgtgaagcgc ttgaggatcg cccgcacgat cgcccgggtc ggcgggatca ggaagagcag
     1861 gccgacgatg tcggtgatga agacgggcgc cagcagcagc gtgccgccga tcacgatcag
     1921 cagcccgtcg acgacctcgg tgcccgggaa gcgaccctgc gccagcgcct cgttgaagcg
     1981 gcgccaggcg ccgcgcccct ggtggcgcag cagcagcgag ccgaggatcg cgtcgaggaa
     2041 gagcagcgcc agggtcggca ggatgccgat cgcttcgccg accttgatga tcacgtacag
     2101 ctccgcgatc gggacgacga tgaacagaat caccaagaag aacatggacc aagcgtaggg
     2161 aactggatcc gccctcccgg gtttgaacgc cctattgccc gtcgaaaccc gggagcgcgt
     2221 caggctgcgg cggcggactc gctggccgcg gcctcgagct cggcgagcga ggcgttgagg
     2281 ctttcggcga ggagttcgat gtcctccatc gagtcgtagt cggcgagcag gccgaagccg
     2341 accttgccgt tgtagctcat gatcgccacc gccagcgtgt ggttctcggg caggaaggca
     2401 atcgggaaga cctcctccag ttcgcgcccg agcacgtaga ggggcagctg cgggcccggc
     2461 acgttggtga cgatcatgtt gaagagccgg gtcgagaagt tgatccgcga ggcctgcgcc
     2521 agcagggtcg gcggcgcgaa gtcgttgaag cgcgagatca cttccgcgcc gagcgcctgc
     2581 ttggagcgct tcagcccctc catcgcctgg gtgatcgtgc gcaggcggcg aaccgggtcc
     2641 tcgacgtaga ccggcaacgg cccccgcatc agcgcgatcc ggttgccgag gttgccgcgc
     2701 tcgtcctcgc cgcggatcga gaccgggacc agcgcccgca gctccaaccc ctcggtgcgg
     2761 atgctgcgac cgtgcagcca ctcgcgcacc gcgccggtga cgaccgcgag caccacgtcg
     2821 ttgacggtcc ccccgagcga gtccttgatc ttcttgaagg tcgccagctc cgagcgcgcc
     2881 cagacgtagc gccggtgcgg cccgatcttc tggttcagcg gcacgtccgg ggcggggtcg
     2941 gcgaaggcgc tgacgatctc gcttatcccc tccagggcct cgaccgcctt gcgcgccgcg
     3001 cgctccggct tcttcaccgc ggagaccgcg cgctcggcga acttgaccgg caccgcggcg
     3061 aagtcgctga ccccacgggc gaccagctcg gtggtgcccg gttcgggctg cggtacccac
     3121 tcgtcggcga tctcgaccgg gtcggggacc ggctcgaggt cgaacagcac ggtgccgatg
     3181 tcgacgccgc tgaccccgtc gaccatcgcg tggtgggtct tggtcaggat cgcgaagcgg
     3241 tcgcgctcca ggccctgcgc cagccacagc tcccagagcg gcttcgagcg atcgagctgc
     3301 tgcgagaaga cccgcccggc cagccgtttg agctgcgctt cgccgcccgg atcgggcagc
     3361 gcggtgtggc ggatgtggta ggtcaggttg aagttgacgt cgtcggccca gagcggccgg
     3421 cccgcctcca gcggcggcac gaccagcttc tggcggaagc gcgggaccag cgccaggcgg
     3481 ccgcgcacgt gctcgaccag gtcgacgtat ttcggcggcg gcccctcgaa gatcaggatc
     3541 gcgcccacgt gcatgtggct ggccgagctc tcgttcgtca ggaacgaggc gtcgacggcg
     3601 gtcaagcgat ccaggtggcg cccctccgtg ctcattcgca caggctaacc tcgcacgcct
     3661 ttcagggcca gccgcgggcg gcggccgcaa gtgcgccacc gctaccctgg gggcatgccc
     3721 gaagccacca tcgcggcccc caagcgcgtg ctcctggccg ctccgcgcgg ctactgcgcc
     3781 ggcgtcgacc gtgccgtgca gaccgtcgag cgggccctgg agctgcacgg ggcgccggtc
     3841 tacgtccgca aggagatcgt ccacaacaag cacgtggtcg aagaactggc caagcgcggc
     3901 gcgatcttcg tcgaggagga gaccgaggtg ccggagggcg cgatcgtcgt cttctccgcc
     3961 cacggggtcg ccccctcggt gcacgagaac gccgcctccc gcgggctgca gacgatcgac
     4021 gcgacctgcc cgctggtcac caaggtccac gtctccgccc gccgcttcgc cgcccagggc
     4081 tacacgatcg tgatgatcgg ccacgacggc cacgaggagg tcgaaggcac gaccggcgag
     4141 gcgccggaca gcatcgtcct gatcgaaacc gccgacgagg tcgcctcgct cgaggtcccc
     4201 gatcccgaga aagtcgcctt catcacccag acgacgctct cggtcgacga gaccgccgag
     4261 gtgatcgccg ccctgcgcgc caagttcccc tcgatcgtca gctccaagtc agacgacatc
     4321 tgttacgcga cgaccaaccg ccagatcgcg gtcaagcagc tcgcccgcga gtgcgacctg
     4381 gtcctggtga tcggctcgac caactcctcc aactccaacc gcctggtcga ggtcgcgcgc
     4441 gaacacggcg ccggctcgca cctgatcgac aaccacaccc aggtccagga ggagtggctg
     4501 gcgggcgtcg agaccgtcgg catcacctcc ggggcgagcg cccccgagga gctggtcgag
     4561 agcctcgtct ccttcttccg cgagcgcggc gccgacgacg tctccgagct gcgcaccgtg
     4621 cacgaggacg tccgcttcat gctccccaag gagatccgtg aggagctcgc ggcgcgggcc
     4681 tagggctcgt cggcggcgct ggggggcgcc gggttgtctc gccgcagcgc tcgccgcggc
     4741 cgcgctgctc tgcgcgccgg cgcttgccgc cgacgccggg ccgcttccgt tctgcgggcc
     4801 cgacgaagcc gaagaacgcg aggcgcggct ggcggcgatg ccgggcgccc ggcccgcgcc
     4861 cgaaggaccg cttcccttcg gtccccgcaa cttcaacatt cacaaggcgg accgtaccgc
     4921 ggtggttctg gagggaagcc gcatcggcta ccgcttcggc gcgaaaaacg gctactcccg
     4981 ggagctggtc ctgaattggc gcctcggcgc gaccctgcgg cgggtcgact ccgccgggcg
     5041 ggtgaagcgg gcgctggggc ggcgccacgt cggcgtcggc aaggtcgacg acctcgagca
     5101 gatggacctc ctctttcccg ccgagcaccc gggcctctac cggatcgaca cgcggatacg
     5161 agacctcagc agcggcaaga gggtcagcta ccgcgagtac ttcgaggtcc tgaagcgcag
     5221 cgtcgacgta agcatcgccg tcagcgtccc gagcgcgcgg cccggcgaag ccgtttacgg
     5281 tcgcgtcgtc aaccgcggtg ggaccaacct gaccggggtt ccggccttcc tcgacgtcga
     5341 gcgcgagcag ggctccacct gggtgccggt agagcagccg ccgaccccgg cctcggtgct
     5401 ggacttccac tggtggatgc acagcggcga agcgacgtac tgcagccgct tcgacgtccc
     5461 gtcggatgcg acccccggcc gttaccggtt caccgcctcg atctacgtcg tcaacctgca
     5521 gcgccgcgag accttcaccg gcggcttcga agtcgctccc tgatcctcag cgcggcttgc
     5581 ctcgcaggat gcggaagcgg taggtcgtg
//
LOCUS       DASRIG010000119         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_582687, whole genome shotgun sequence.
ACCESSION   DASRIG010000119 DASRIG010000000
VERSION     DASRIG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582687"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFB52_03270"
     CDS             complement(<1..424)
                     /locus_tag="VFB52_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZO05380.1"
                     /translation="MAATDRTASEGTHRTIAPLVGPDDDRVFTDSGIEIEQLYGPEDV
                     APGLEERLGDPGEPPFTRGIHEGMYRDRLWTMRQYAGFASPEDTNERYRYLIEHGSTG
                     LSMAFDLPTQLGRDSDDPLCHGEVGRTGVPIDTIEDMRI"
     gene            602..1810
                     /locus_tag="VFB52_03275"
     CDS             602..1810
                     /locus_tag="VFB52_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05381.1"
                     /translation="MIDASQPRLARGKLPAGILATALIALLAFAPLASASPDPVGSGS
                     TTVTLNNGWVNYLKTFGIKVQKEGATKIKGKAATFTNTGGSLDPTTGLGEVTLGGGLK
                     FKAGKKTAKVNGLSLDTNKKALFAKVSGKKVKFAAIAGWSQTRNGFGVNLTINKLKLT
                     NAAATQLNKKTGYAKGKPKPFLKNKLIGKGTSDVQPSTVAVVPTGTASLALSIQAVEK
                     LSKVGPEMAPGVWPFAVKLGIAPPTTVTNPGPPPTVAFPLGPEGTIGPAGNSGVIKTL
                     GGLTLTQDLELAGKGTTTLTMGNIWIDLGTKQASVEVTIANPKTPEANLGNLGRASIA
                     DFVVGPVTIDSANRTVSVTNASATLQAVTAETLNQVFIKPLEGTFGPDDVQFAAGDPL
                     GTFSFTAQGQ"
     gene            complement(1869..2816)
                     /locus_tag="VFB52_03280"
     CDS             complement(1869..2816)
                     /locus_tag="VFB52_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZO05382.1"
                     /translation="MSTCVVTGGAGFLGSHLCEHLLGQGHRVICIDNLETGSLENIAH
                     IRDEAFDFRNIDITSYVDIPEDVDFVYHLASPASPIDYLRLPLHTLKVGSQGTHNMLG
                     VAKFKRARFLTASTSEVYGDPQVHPQKEDYWGHVNPIGPRGVYDEAKRYAEALVMAYH
                     RQQGLDTAIVRIFNTYGPRMRPHDGRAIPTFLRQALQDRPLTVFGDGSQTRSFCYVDD
                     LIRGIVALAESDVHTPVNIGNPDEFTLLELAKAVIEVTESRSEIVYEALPTDDPQQRR
                     PDIGRARDLLGWEPKVALLEGLRRTIDQSGIERLVGATD"
     gene            2987..4300
                     /locus_tag="VFB52_03285"
     CDS             2987..4300
                     /locus_tag="VFB52_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO05383.1"
                     /translation="MSQSCEQGDGAGLAREAARPEQAARKRAAVETYARHEKTLRRQA
                     RRFSLCEDDADDALQRALEILLRKAPTTDQRELIRWTQTVVKHEALAIRRERERILAG
                     PAAASAEDEIDWVALIPAADAGPAERAERSEAIARSREALQALKPQELRALGLLAEGY
                     SYREIGEITGYSQTKINRCLAEGRERFRSLLARSEDGRRCAEMGPQLSAFCDGEANAN
                     DEAELREHLRACSHCRATLRAYRAVPAAAAALAPTLPIHRSLFERAHDAALGLAARFG
                     GGGGDSAISGVAATGGSRGAGMAALAKVLAVCAGTVGGAAACAATGVIPAPLALDSGR
                     DEPRIERRAEQPEKASASTTEAPVEYEPETPVAEPTPSGNADGGGKGDEQRTEPAPEP
                     TPEPVAESGAVEYAPPPPPVATSSESSSAGSSNSGGGDPAGEFSP"
     gene            4297..>4929
                     /locus_tag="VFB52_03290"
     CDS             4297..>4929
                     /locus_tag="VFB52_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05384.1"
                     /translation="MSRLLAATAAALASLTIVVAPFATASGLNPAEDWRVADLTVNDR
                     AAEWYADSAFRLTWSLTPKLLAASDLVGSISYRLRGAGGQVLRVEQLGAEARSVTVNL
                     PGEPGEPAPHNGEYSVEVWVNGAVPTDKPWLGPGEIEQLRFDNARPGAAIPQLATTWV
                     RGDTEPAVRIAHPVGPEPLSGIRGYAVALHRDTAAPPCAGPERCSVAETDL"
BASE COUNT          830 a         1693 c         1713 g          693 t
ORIGIN      
        1 agatccgcat gtcctcgatc gtgtcgatcg ggacaccggt gcggccgacc tcgccgtggc
       61 agagcgggtc gtcggagtcg cggccgagct gggtcggcag gtcgaaggcc atcgagagcc
      121 cggtcgaacc gtgctcgatc aggtagcggt agcgctcgtt ggtgtcctcg ggcgaggcga
      181 agccggcgta ctggcgcatc gtccagagcc ggtcccggta catcccctcg tggatcccgc
      241 gcgtgaacgg cggctcgccc gggtcgccga gtcgctcctc caagccgggg gcgacgtcct
      301 cgggcccgta gagctgttcg atctcgatcc cggagtcggt gaagacccgg tcgtcgtcgg
      361 gccccaccag gggggcgatc gtgcggtggg tcccctcgga cgccgtgcgg tcagtcgcgg
      421 ccatcgtggg caggatattg cggatcgact ccgggccgct tccagagcgg ttaaccggaa
      481 ccttggctgg ccagccgggt tgtcttatac ggcggccagg aataggcttg accgcaatac
      541 aactttgccg cgcgtcgcct aatcgggacc aacagggggc ggcaaacaac ggaggaacta
      601 catgattgat gcatcccagc cacgcctggc tagggggaag ctgcccgccg ggatcctggc
      661 gactgcgctg atcgcgctgc tggcgtttgc ccccctcgct tccgcgtcgc ccgacccggt
      721 cggcagcggc agcaccaccg tgaccctcaa caacgggtgg gtcaactacc tcaaaacctt
      781 cggcatcaag gtgcagaagg aaggggccac caaaatcaaa ggcaaagcgg ccaccttcac
      841 caacaccggc ggctcgctcg acccgaccac cggactgggt gaagtcaccc tcggcggcgg
      901 actcaaattc aaggccggca aaaagaccgc caaggtcaac ggcctcagcc tcgacaccaa
      961 caaaaaagcg ctgttcgcca aagtcagcgg caagaaagtc aagttcgccg cgatcgccgg
     1021 ctggtcgcag acccgcaacg ggttcggcgt caacctgacg atcaacaagc tgaagctgac
     1081 caacgctgcc gccacccagc tgaacaagaa gaccggctac gccaaaggca agccgaagcc
     1141 cttcctgaag aacaagctga tcggcaaagg cacgagcgac gtccagccga gcaccgtcgc
     1201 ggtcgttccg accgggaccg ccagcctcgc gctgagcatc caggcagttg aaaaactgtc
     1261 gaaagtcggc cccgaaatgg cccctggcgt ctggccgttc gcggtcaaac tcgggatcgc
     1321 cccgccgacc acggtgacca acccgggccc gccgccgacc gtggcgttcc cgctcggccc
     1381 cgaaggcacg atcggcccgg ctggtaactc cggtgtgatc aagaccctcg gcggtctcac
     1441 cctgacccag gacctggaac tcgccggtaa aggcaccacc accctgacga tgggcaacat
     1501 ctggatcgac ctgggcacca aacaggcctc ggttgaagtc acgatcgcca acccgaaaac
     1561 cccggaagcc aacctcggca acctgggccg ggcctcgatc gccgacttcg tcgtcggtcc
     1621 ggtgacgatt gattccgcca accgcacggt cagcgtcacc aacgcgtcgg ccaccctgca
     1681 ggcggtcacc gcggagaccc tgaaccaggt gttcatcaaa ccgctcgaag ggaccttcgg
     1741 tcctgacgac gtgcagttcg ctgccggcga tccgctcggc accttctcct tcaccgccca
     1801 gggtcagtaa cccaaggcgc aagggaagct cagaaaaggg cggcctccgg gccgcccttt
     1861 tttatgcctc agtcggtggc gccgacgagg cgctcgatgc cggactggtc gatcgtccgg
     1921 cgcaggccct cgagcagggc gactttcggt tcccagccga gcaggtcgcg ggcgcggccg
     1981 atgtcggggc ggcgctgctg ggggtcgtcg gtgggcagcg cctcgtagac gatctccgag
     2041 cgcgactcgg tcacctcgat cacggctttc gccagttcga gcagggtgaa ctcgtccggg
     2101 ttgccgatgt tgaccggcgt gtgcacgtcg gactcggcga gcgcgacgat gccgcggatc
     2161 aggtcgtcga cgtaacagaa gctgcgcgtc tggctgccgt ctccgaacac ggtcagcggc
     2221 cggtcctgga gagcctggcg caggaaggtc gggatcgcgc ggccgtcgtg cgggcgcatg
     2281 cgcgggccgt aggtgttgaa gatgcggacg atcgcggtgt cgagcccctg ctggcggtgg
     2341 taggccatca ccagggcctc ggcgtagcgc ttcgcctcgt cgtagacacc gcgcgggccg
     2401 atcgggttga cgtgccccca gtagtcctcc ttctgcgggt ggacctgggg gtcgccgtag
     2461 acctcgctgg tcgaagcggt gaggaaacgg gcgcgtttga acttggcgac gccgagcatg
     2521 ttgtgcgtgc cttgcgaacc gaccttgagc gtgtgcagcg gcagccgcag gtagtcgatc
     2581 gggctcgccg gcgaggccag gtggtagacg aagtcgacgt cctcggggat gtcgacgtag
     2641 ctggtgatgt cgatgttgcg gaagtcgaac gcctcgtcgc ggatgtgggc gatgttctcc
     2701 aacgacccgg tctcgaggtt gtcgatgcag atcacccggt gcccctgccc gagcaggtgc
     2761 tcgcagaggt gtgaaccgag gaatccggcg ccgccggtaa ctacgcaggt gctcatcgcg
     2821 ccgatgctac ctgcctgagt ccgatccgtg ctccgcaccg actcgttgca atttgcgtga
     2881 tttcggcgcg gatttgtgcc tgagaggggg gagagaagac gcacgcaggg gctttccgtg
     2941 aaatgtttgg tacagaggag ttcattggcc agggggagcc aaagcggtga gtcagagttg
     3001 tgagcagggg gacggcgccg ggctggcgcg ggaggcggca aggccggagc aggccgcgcg
     3061 caagcgcgcc gcggtcgaga cctacgcgcg ccacgagaag acgctgcggc gccaggcgcg
     3121 gcgcttctca ctctgcgagg acgacgccga cgacgcgctc caacgagcgc tggagatcct
     3181 cctgcgcaaa gcgccgacca ccgaccagcg cgagctgatc cgctggaccc agaccgtggt
     3241 caagcacgag gcactggcga tccggcgcga gcgcgagcgc atcctcgccg gccccgccgc
     3301 cgcctccgcc gaggacgaga tcgactgggt cgcgctgatc ccggccgctg acgccggccc
     3361 cgccgagcgg gccgagcgca gcgaggcgat cgcgcgcagc cgcgaggcgc tgcaagcgct
     3421 gaagccccag gagttgcgcg cgctcgggct gctcgccgag ggctactcct accgcgagat
     3481 cggtgagatc accggctact cgcagaccaa aatcaaccgc tgcctggccg agggccgcga
     3541 gcgcttccgc agcctgctgg cgcgcagcga ggacggtcgc cgctgcgccg agatggggcc
     3601 gcagctctcc gccttctgcg acggcgaggc gaacgccaat gacgaggcgg agctgcgcga
     3661 gcacctgcgc gcctgctcgc actgtcgcgc cacgctgcgc gcttaccggg cggtcccggc
     3721 tgcggcggcg gcgctggccc cgaccctgcc gatccaccgc tcgctgttcg aacgcgccca
     3781 cgacgccgcg ctcgggctcg ccgcccgctt cggcggcgga ggcggcgact cggcgatctc
     3841 cggggtcgcc gccacgggtg gctcgcgcgg cgccggcatg gcggcgctgg cgaaggtgct
     3901 cgcggtctgc gccgggaccg tcggtggtgc cgcggcctgc gccgccaccg gggtgatccc
     3961 ggcgccgctg gcgctcgact ccggtcggga cgagccacgg atcgagcgcc gtgccgagca
     4021 gccggagaag gcgagcgcga gcaccaccga agccccggtc gaatacgagc cggaaacgcc
     4081 ggtagccgaa ccgacgccga gcggaaacgc cgacggtggc ggcaaaggcg acgagcagcg
     4141 cacggaaccg gcgcccgaac cgaccccgga accggtcgcc gaaagcggcg cggtcgaata
     4201 cgcccctccc ccgccgccgg tcgcgaccag ctcggaatcg agctccgccg gcagctccaa
     4261 ctccgggggc ggcgacccgg ccggggagtt cagtccgtga gccgcctgct cgccgcgacc
     4321 gccgcggctc tcgcctcgct caccatcgtc gtggctccat tcgccaccgc gtccggcctg
     4381 aacccggccg aagactggcg cgtcgccgac ctaaccgtga acgacagggc agcggagtgg
     4441 tacgccgaca gtgcattcag gcttacctgg agcctcaccc cgaagctgct cgcggcgtcg
     4501 gacctggtgg gctcgatctc ctatcgcctg cgcggtgccg ggggacaggt gctgagggtc
     4561 gagcaactcg gcgccgaggc gagatcggtg accgtcaacc tcccgggcga gccgggcgag
     4621 ccggccccgc acaacggcga atacagcgtc gaggtttggg tcaacggagc ggtgccgacc
     4681 gacaaaccat ggctgggccc aggcgaaatc gagcagttgc ggttcgacaa cgcgcgcccc
     4741 ggcgcggcga tcccgcagct cgcgacgacc tgggtccggg gggacaccga accggcggtg
     4801 cggatcgcgc atccggtcgg ccccgaaccg ctctcgggta tccgtggcta cgccgtcgcg
     4861 ctgcatcgcg acaccgccgc gccaccatgc gccgggccgg aacggtgcag cgtggcggag
     4921 accgacctc
//
LOCUS       DASRIG010000120         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_58656, whole genome shotgun sequence.
ACCESSION   DASRIG010000120 DASRIG010000000
VERSION     DASRIG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58656"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..855
                     /locus_tag="VFB52_03295"
     CDS             64..855
                     /locus_tag="VFB52_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05385.1"
                     /translation="MSQKARKKLRPPDLAGSSWSARLRNFTFAMFGLTAIVCLSFVAF
                     VAQVSRPVLSVAPLPLPGESLTAPGDSFGLGDGIAGGIGAGFGGLGVPGFGGDSGPAT
                     AVAGLGGDESSSVFAGLGSNGGSPDSGAGSLGNNPGLGSGANSGPVDVTPAHEPTEPP
                     AAVPSATPVSGSDSSSGLYVRTSVAPGKGEDAGPPPDEPETPAEPETPVEPEVPVEPE
                     PPVEPPVEPEVPVEPEFPFEPPVEEEPAPEPAPEEVPAPEVPAGQ"
     gene            879..1847
                     /gene="holA"
                     /locus_tag="VFB52_03300"
     CDS             879..1847
                     /gene="holA"
                     /locus_tag="VFB52_03300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZO05386.1"
                     /translation="MKPLYLIAGTDAAKIDATRARLRARAEREGGAASLEVFELSEGR
                     GMPDHEALLMAIPAMSLMDSRRYLLVDGAERWRDKQLDPVAAAVAELPPDLTLVLIAR
                     AKAPPKLLKAVKAAKGEVHEFEAPKARDMPRVLVADAQGLGFRLDPAAARVLVDRMGA
                     NPLRLRNELERLALWAGEGGAVSADDLDAMIADTSEAAVWSLSDALLEGDAATALRIG
                     EQLISQGENITGLIYGLASRLRQACAAAAKLEEGVPPAQVESSLKMHPYAAKQLVRRL
                     NNANLADLRLATEALAELELWCRGEKDYGDELALTLALRSMATQSA"
     gene            complement(1921..2181)
                     /gene="rpsT"
                     /locus_tag="VFB52_03305"
     CDS             complement(1921..2181)
                     /gene="rpsT"
                     /locus_tag="VFB52_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZO05387.1"
                     /translation="MANIASQKKRILRSERERTENRLLTSTVKTHFRRLESAVEGGDA
                     STIAEEQRVLTSKIDKAVQKGAMHRNTGARKKSKAARIAQSA"
     gene            2248..>3029
                     /locus_tag="VFB52_03310"
     CDS             2248..>3029
                     /locus_tag="VFB52_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZO05388.1"
                     /translation="MEIPDTPAGNERAINADEEQVVSEVALRQAEAFDPGEELSAEPA
                     PGSQAGRIARSTAFFSIATAASRVVGLLREVIAAGFYGIKGPMSAFTIAFQVPNLIRA
                     LFADAALQPAFVPVFTELLEKKSYREAFRLASTLLMIVTMVLGAITALFVLLAPVVMP
                     LFAPGFKGEILDLTVSLSQVLFPILVLLGISGVVVGILNSYDRFGAFAISPLFWNLTI
                     ILVLVLIEPLFHGQDRIYAYAIGILVGTAVQLAIPAWDLRNTP"
BASE COUNT          454 a         1087 c         1006 g          482 t
ORIGIN      
        1 gaaacttgaa caagattggg ctacacctac cgagacgccg ctctggggtc gatccttagt
       61 tcgatgagcc agaaagcgcg aaagaagctg cggccgccgg atctggctgg ctcgagctgg
      121 tcggcaaggc tgcgcaactt caccttcgcg atgtttggcc tcaccgcgat cgtctgcctc
      181 tccttcgttg ccttcgtcgc ccaggtcagc cgtccggtcc tctcggtcgc gccactgccg
      241 ctcccgggcg agtcgcttac cgcgccgggc gacagcttcg gcctcggcga cgggatcgcc
      301 ggggggatcg gcgccggctt cggcggcctc ggagtgccgg ggttcggcgg cgactcgggc
      361 ccggccaccg cggttgccgg cctcggcggc gatgaaagct cgtccgtctt cgccggtctc
      421 ggtagcaacg gcggctcccc cgacagcggt gccggctcgc ttggcaacaa tccggggctt
      481 ggcagcggag cgaactcggg tccggtggac gtgacgccgg cgcacgagcc gaccgaaccg
      541 cccgcggccg ttccgtcggc cactcccgtg tctgggtccg actcctccag cggcctttac
      601 gtgcgcacct cggtggcccc gggcaaaggc gaagacgccg gtccgccgcc ggatgaaccc
      661 gaaaccccgg cagaacccga aacgccggtc gaaccggaag tcccggtcga acccgaaccg
      721 ccggttgaac cgccggtcga acccgaagtg ccggtcgaac cggaattccc gttcgaaccg
      781 ccggtggaag aagaacccgc ccccgaaccg gcgcccgaag aagttccggc ccccgaagta
      841 ccggcgggcc agtaactaga ctcggccgcg tggccgagat gaagccgctg tacctgatcg
      901 ccggcaccga cgcggcgaaa atcgacgcga ccagggcgcg tctgcgggcc cgcgccgagc
      961 gcgagggagg cgccgcgtcc ctcgaggtct ttgagctctc cgagggcagg ggcatgcccg
     1021 accacgaggc gctgctgatg gcgatcccgg cgatgtcgct gatggactcg cgccgctacc
     1081 tgctcgtcga cggggcggag agatggcgtg acaaacagct cgacccggtt gccgccgcgg
     1141 tcgccgagct gccccccgac ctaaccctgg tcctgatcgc gcgcgcgaaa gcgccgccga
     1201 agctgctgaa agcggtgaaa gccgcgaaag gcgaggtcca cgagttcgag gcgccgaagg
     1261 cgcgcgacat gccgcgcgtg ctcgtcgccg acgcccaggg tctcggcttt cgcctcgacc
     1321 cggccgcggc ccgggtcctg gtcgaccgca tgggcgccaa cccgctgcgc ctgcgcaacg
     1381 agctggagcg gctggcgctc tgggcggggg agggaggagc ggtgagcgcc gacgacctcg
     1441 acgcgatgat cgccgacacc tccgaagccg cggtctggtc gctctccgac gccctgctcg
     1501 aaggcgacgc cgccacggcc ctgcggatcg gcgagcagct gatctcccag ggcgagaaca
     1561 tcaccgggct gatctacggc ctcgcttcgc gcctgcgcca ggcctgcgcc gctgccgcca
     1621 agctcgagga gggcgtgccg cccgcccagg tcgagtcctc gctgaaaatg cacccgtacg
     1681 ccgccaagca actggtccga cgcctcaaca acgcgaacct cgcggatctg cgcctggcga
     1741 ccgaggcgct agcggaactg gagctgtggt gccgaggaga gaaggattac ggcgacgagc
     1801 tcgcgctcac gctcgcactc aggtcgatgg cgacccagag cgcgtaacca agcagatcaa
     1861 ggacgcaggg agcgtggcgt gctctgcacg cgagcgaccg aggacgccga tatgcgccgg
     1921 ttaggcgctc tgggcgatgc gtgccgcttt ggacttcttg cgagcgccgg tattgcggtg
     1981 catggcgccc ttctggacgg ccttgtcgat cttcgaggtc agcacccgct gctcctcagc
     2041 gatcgtgctc gcgtcaccgc cctcaaccgc gctctcgagg cgacggaaat gggtcttgac
     2101 cgtgctcgtc aggagccggt tctcggtgcg ctcgcgctcg ctgcgcagta tccgcttctt
     2161 ctgtgaggct atgttggcca tatcgagtgt ccgcgcgggc gccttgccgg ctccgggcgg
     2221 ccgccgacta tagcgactga gaccagcttg gagatcccgg acacccccgc aggcaacgag
     2281 cgcgccataa acgccgacga ggagcaggtc gtctccgagg tcgcgctgcg ccaggcggag
     2341 gccttcgatc cgggcgagga actgtccgcc gagcccgctc ccggcagcca ggccgggcgc
     2401 atcgcccgct ccaccgcctt cttctcgatc gccaccgccg cctcgcgtgt ggtcggcctg
     2461 ctgcgcgagg tgatcgccgc cggcttctat gggatcaaag gcccgatgtc ggccttcacg
     2521 atcgccttcc aggtccccaa cctgatccgc gccctgttcg ccgacgccgc tctgcagccg
     2581 gccttcgtcc cggtcttcac cgaactgctg gagaagaaga gctaccgcga ggcgttccgg
     2641 ctcgcctcga ccctgctgat gatcgtgacg atggtgctgg gcgcgatcac cgcgctcttc
     2701 gtgctgctcg ctccggtcgt gatgccgctg ttcgcgcccg gattcaaggg cgagatcctc
     2761 gacctcaccg tctcgctctc gcaggtcctg ttcccgatcc tcgtcctgct cgggatcagc
     2821 ggcgtcgtcg tcggcatcct caacagctac gaccgcttcg gggccttcgc gatctcgccg
     2881 ctgttctgga acctgacgat catcctcgtg ctcgtcctca tcgagccgct gttccacggc
     2941 caggaccgga tctacgccta cgcgatcggc atcctcgtcg gcaccgccgt ccagttggcg
     3001 atccccgcgt gggacttgcg taacacgcc
//
LOCUS       DASRIG010000121         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1869296, whole genome shotgun sequence.
ACCESSION   DASRIG010000121 DASRIG010000000
VERSION     DASRIG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869296"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFB52_03315"
     CDS             <1..1169
                     /locus_tag="VFB52_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZO05389.1"
                     /translation="QHNKFPLKTRDDLSMAYTPGVARVCMDIHHDRSKAFEYTIKKNT
                     VAVVSDGSAVLGLGNIGPEAAMPVMEGKAVLFKEFADVDAFPICLATQDTEEIIKATE
                     LIAPTFGGINLEDISAPRCFEIEDRLKETLDIPVFHDDQHGTAVVTMAALFNALKIVD
                     KPIEDLRVLMVGLGAAGVAVTKMMLASGVTKIIGCDRQGALSTSREDYASGEMNGMKR
                     WYAEMSNPEQIQGQPADVIDGMDLFIGLSGPGIIEGTDLAKMNDDAIVFAMANPTPEV
                     MPEEAAPYVRIMATGRSDYPNQINNVLAFPGIFRGALDAGASSITEAMKLAAARGIAE
                     TVSDDDLAEDYIIPSVFNREVAPKVAAAVVEEAKRDGIARLNDETGTFATVESP"
     gene            1174..2082
                     /locus_tag="VFB52_03320"
     CDS             1174..2082
                     /locus_tag="VFB52_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZO05390.1"
                     /translation="MKVLVTGASGLIGSALCDALLERDDEVVALSRDPERARGKRPGI
                     VWHRWEPTLERPPAAAFEGVDGVVHLLGEPIDQRWTDAAKERIMETRRTGTRNLVAAI
                     VGLEQRPKVLVSQSAVGYYGDRGDAVVDESTPSGGESFDSEVTQAWERSAREVSASGV
                     RLVIARTGQVLDPSGGMLAEMLTPFKLGVGGPLAGGAQYLSWIHIEDEVGLLLWSLDH
                     EEVTGVVNATAPEPATNKEFSKALGRALGRPAVVPVPGFVLDLKFGSEFGHVLRGGQR
                     VLPKRALELGYQFKHPQLDEALADLV"
     gene            complement(2079..>2565)
                     /locus_tag="VFB52_03325"
     CDS             complement(2079..>2565)
                     /locus_tag="VFB52_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05391.1"
                     /translation="AAVALLALGLAMAGAIGCGEEEGVADGATVHAYVEAPVCSKAAM
                     VAVVGNEDGEGIGVRFICLTAVRGPELSQGVGGGRQIDLAIVGANARRATEDSTTVAY
                     LQADDPAVNRFTEPILEEAGIGTITAPKGKAQSQLLKLVGEADPSSLRADVREALEEE
                     S"
BASE COUNT          424 a          939 c          828 g          374 t
ORIGIN      
        1 agcagcacaa caagttcccg ctgaagacgc gcgacgacct ctcgatggcc tacacgcccg
       61 gcgtcgcccg cgtctgcatg gacatccacc acgaccgcag caaagccttc gagtacacga
      121 tcaagaaaaa caccgtcgcg gtcgtctccg acggcagcgc cgtgctcggg ctcggcaaca
      181 tcggcccgga ggcggcgatg ccggtgatgg agggcaaggc ggtcctgttc aaagagttcg
      241 ccgacgtcga cgccttcccg atctgcctcg ccacccagga caccgaggag atcatcaaag
      301 cgaccgaact gatcgccccc accttcggcg ggatcaacct cgaggacatc tcggcgccgc
      361 gctgcttcga gatcgaggac cggctgaagg aaaccctcga catcccggtc ttccacgacg
      421 accagcacgg caccgcggtg gtgacgatgg cggcgctgtt caacgcgctg aagatcgtcg
      481 acaagccgat cgaggacctg cgggtgctga tggttggcct cggtgcggcg ggggtagcgg
      541 tcaccaagat gatgcttgcc tccggcgtca ccaagatcat cggctgcgac cgccagggcg
      601 cgctctcgac ctcgcgcgag gactacgcct cgggcgagat gaacgggatg aagcgctggt
      661 acgcggagat gtccaacccc gaacagatcc aaggccagcc cgccgacgta atcgacggca
      721 tggacctttt catcggcctc tccggccccg ggatcatcga gggcaccgac ctggcgaaga
      781 tgaacgacga cgcgatcgtc ttcgcgatgg ccaacccgac ccccgaggtg atgcccgagg
      841 aagcggctcc ctacgtgcgg atcatggcca ccggccgctc ggactacccg aaccagatca
      901 acaacgtgct cgccttcccg gggatcttcc gcggcgccct cgacgccggc gcctccagca
      961 tcaccgaggc gatgaagctc gccgccgccc gcgggatcgc cgaaaccgtc tccgacgacg
     1021 acctcgccga ggactacatc atccccagcg tcttcaaccg cgaagtggcg ccgaaggtgg
     1081 cggccgcggt ggtcgaggag gccaagcggg acgggatcgc ccggctcaac gacgagaccg
     1141 ggaccttcgc caccgtggag tcgccctagc tccatgaagg tcctcgtcac cggagccagc
     1201 ggcctgatcg gctcggcgct gtgtgacgca ctgctggagc gagacgacga ggtcgtcgcc
     1261 ctcagccgcg accccgaacg cgcccgcggc aagcgccccg ggatcgtctg gcaccgttgg
     1321 gagccaactc tggaacgacc gccggccgcc gccttcgagg gcgtcgacgg ggtcgtgcac
     1381 ctgctcggcg agccgatcga ccagcgctgg accgacgccg ccaaggagcg gatcatggag
     1441 acccgccgca caggcacccg caacctggtc gcggcgatcg tcgggctcga gcagcgcccc
     1501 aaggtcctgg tcagccagtc ggcggtcggt tactacggcg accgcggcga cgccgtcgtc
     1561 gacgagtcga ccccgtccgg cggcgagagc ttcgactccg aagtgacgca ggcctgggag
     1621 cgatccgcgc gcgaggtctc ggccagcgga gtgcgcctcg tgatcgcccg caccggccag
     1681 gtgctcgacc cctccggcgg catgctggcc gagatgctga ccccgttcaa gctcggcgtc
     1741 ggcggcccgc tcgccggcgg cgcccagtac ctctcctgga tccacatcga ggacgaggtc
     1801 ggactcctgc tctggtcgct cgaccacgag gaggtgaccg gcgtggtcaa cgcgaccgcg
     1861 ccggagccgg cgaccaacaa ggagttctcg aaggcgctcg gccgcgccct cggccgcccc
     1921 gcggtcgtcc ccgtgcccgg cttcgtcctc gacctcaaat tcggctcaga gttcggccac
     1981 gtcctgcgcg gcggccagcg ggtgctgccg aaacgggcgc tggagctggg ctaccagttc
     2041 aagcacccgc agctcgacga ggcgctggcc gacctggtct agctctcttc ttcgagcgcc
     2101 tcgcgcacgt cggcgcgcag tgagctcggg tcggcttccc cgaccagctt cagcagctgg
     2161 gactgtgctt tccctttggg tgcggtgatc gtgccgatcc cggcttcttc gaggatcggc
     2221 tcggtgaagc ggttgaccgc gggatcgtcg gcctgcaggt aggcgaccgt ggtcgagtcc
     2281 tcggtggccc ggcgtgcatt tgcgccgacg atcgccaggt cgatctgtcg gccacccccc
     2341 acgccctggc tcagctcggg accacggacc gcggtaaggc agatgaacct caccccgatc
     2401 ccctcgccgt cctcgtttcc gaccaccgcc accattgccg ctttgctgca cacgggcgct
     2461 tccacgtacg cgtgcacggt cgccccgtcg gcgacgcctt cctcctcccc acaaccgatg
     2521 gcccccgcca tcgccaaccc gagcgccagc agcgccaccg ctgct
//
LOCUS       DASRIG010000122         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1363054, whole genome shotgun sequence.
ACCESSION   DASRIG010000122 DASRIG010000000
VERSION     DASRIG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363054"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFB52_03330"
     CDS             <1..1267
                     /locus_tag="VFB52_03330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZO05392.1"
                     /translation="DRSSQPLTRAAGHRVCGSLAQLRVRPGARIAVELPPCAGDTRWA
                     LPTQTERGKLRLRGNRLVYRAPKKFRGTVSANLMAAPAPGIPATRKTAPAVPVQITIG
                     TPKAPVVRALGDSVTAGFGYYEHGELMGISSLLSCRPGETSYDDACSSNSKVRSNKVA
                     KVEYASDYGLSNNVSWAAQWANTHGVTDFKNFAVSGSEPSDWYGKGQFAATTKQLIGE
                     DPDYVLLTMGANPLLSNMLFGIGDIGCGIWADVTGRFKQCIEEEFAAIKLHENLKSLY
                     TTLVKGTDAEIFVMQYHLSIPTAALYGVEQIAQMGAMMNEEIAKAAKEVSPTRITVVS
                     PPHFNVGLSLGQVYPSKYKCEGELFSSIVDGRSVQSTASQDEMEGLHPVEFCAAEEGE
                     EPWVISGDTGIHPSADGYTQMAAKVPPPK"
     gene            complement(1260..1766)
                     /locus_tag="VFB52_03335"
     CDS             complement(1260..1766)
                     /locus_tag="VFB52_03335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZO05393.1"
                     /translation="MEELSSEKVHAGHTVDVSVKRYRRADGTEVTREVVEHPGSVAIL
                     AYDDEVVYLVRQPREAIEDDALLEIPAGTLEPGEGELECAQRELAEEVGLAAEHWHEL
                     KVVHTSPGVLSERATIFAATGLSPASGEQDADEQIDVVRLPRAEVKAALEGITDATTV
                     VALLMLPT"
     gene            1957..2130
                     /locus_tag="VFB52_03340"
     CDS             1957..2130
                     /locus_tag="VFB52_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05394.1"
                     /translation="MATRREGSTGGIGGISLGGILVIIGIVLMIVWSFWIGLIITLVG
                     LIAFGGFAKGKWY"
     gene            2151..2747
                     /locus_tag="VFB52_03345"
     CDS             2151..2747
                     /locus_tag="VFB52_03345"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05395.1"
                     /translation="MAATRGRQVEEPKLSREAILDEAALLFKRNGYRKTRLEELAQVL
                     GVTRPAIYYHFKAKQDLLVAMYMRSMEALNGIAAGSEEDEGLPPVEQFWRRIEAHAIY
                     IASYPVEAGIFFEEEEELPEDVHAEVRRMLVAYTDRLVSFYSAAQKAGDAPEGDPRVA
                     VSAILGGAIWTYRWYPGSDWDDPRAAAREVVRVLRSAS"
     gene            complement(2748..>2890)
                     /locus_tag="VFB52_03350"
     CDS             complement(2748..>2890)
                     /locus_tag="VFB52_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HZO05396.1"
                     /translation="GHPLGSTGARLTTSLLHELERSDKSLGLVTMCCGGGLGTGTLIE
                     RV"
BASE COUNT          505 a          987 c          963 g          435 t
ORIGIN      
        1 cgaccgctcc tcacagccgt tgacgcgggc ggccggccac cgagtctgcg gctcgctggc
       61 gcagctgcgg gtccggcccg gtgcgcggat cgccgtcgag ctgccgccct gcgccggcga
      121 cacccgctgg gcgctgccga cccaaaccga gcgtggcaag ctgcggctgc gcggcaaccg
      181 gctcgtctac cgagcgccga agaagttccg cggcaccgtc tcggcgaacc tgatggcagc
      241 ccccgcgccc ggcatcccgg cgacgcggaa gaccgcgccg gcggtcccgg tgcagatcac
      301 catcggcact cccaaggcac cggtcgtgcg tgcgctcggc gactcggtca ccgccggctt
      361 cggctactac gaacacggcg aactgatggg catctccagc ctgctctcct gcaggccggg
      421 ggaaacgagc tacgacgacg cctgctcctc gaactcgaaa gtccgcagca acaaggtcgc
      481 caaagtcgaa tacgcgagcg actacggcct ctccaacaac gtctcctggg ccgcgcagtg
      541 ggcgaacacg cacggcgtca ccgacttcaa gaacttcgcg gtcagcggct ccgaaccctc
      601 ggactggtac ggcaaaggcc agttcgccgc gaccacgaag cagctgatcg gcgaagaccc
      661 cgactacgtg ctgctgacga tgggtgccaa cccgctgctc tccaacatgc tgttcgggat
      721 cggcgacatc ggctgcggga tctgggccga cgtcaccggc cgcttcaagc agtgcatcga
      781 agaagaattc gccgcgatca agctgcacga aaacctcaag tcgctctaca ccaccctggt
      841 caaaggcacc gacgccgaga tcttcgtcat gcagtaccac ctctcgatcc cgaccgccgc
      901 cctctacggg gtcgaacaga tcgcgcagat gggcgcgatg atgaatgaag aaatcgccaa
      961 agcggcaaag gaagtaagcc cgacccggat caccgtggtc agcccgccgc acttcaacgt
     1021 cggcctctcg ctcggccagg tctacccctc gaagtacaag tgcgaaggcg agctgttcag
     1081 ctcgatcgtc gacgggcgca gcgtccagtc aacggcctcc caggacgaga tggaggggct
     1141 gcacccggtc gagttctgcg ccgccgaaga aggcgaagaa ccgtgggtga tcagcggcga
     1201 caccggcatc cacccgagcg ccgacggcta cacccagatg gccgccaaag tcccgccgcc
     1261 taagtaggaa gcatcagcag cgcgaccacg gtggtcgcgt cggtgatccc ctcgagcgcc
     1321 gccttcacct cggcgcgggg cagccgcacg acgtcgatct gctcgtcggc gtcctgctcg
     1381 ccgctggccg gcgagagccc ggtggcggcg aagatcgtcg cccgctcgct gagcaccccg
     1441 gggctggtgt ggacgacctt gagctcgtgc caatgctcgg cggccagccc gacctcctcg
     1501 gccagctccc gctgcgcgca ctccagctcc ccctcccccg gctccagcgt gcccgccggg
     1561 atctccagca gcgcgtcgtc ctcgatcgcc tcgcgcggct ggcggaccag gtagacgacc
     1621 tcgtcgtcgt aggcgaggat cgcgaccgag cccggatgct cgaccacctc gcgggtcacc
     1681 tcggtcccgt cggcgcgccg gtagcgcttt accgacacgt cgacggtgtg gcccgcgtgg
     1741 accttctccg agctgagttc ctccatcgtt tctccgcgtt caagatcgtg gctggaatgg
     1801 cgcaacctag ctgacatcgc gagaaaccga gatgttttcg acgggcgtgt ttagcgcctc
     1861 agcgatctgg ggtacggcag ataaatcggg gcgcctcttg ggatctgcga gcgatctcgg
     1921 tggcgccccg atttattcca agggaaggag aatcggatgg caacccggag agaggggagc
     1981 accggcggca tcggagggat ctcgctcggc gggatcctgg tgatcatcgg gatcgtgctg
     2041 atgatcgttt ggagcttctg gatcggcctg atcatcaccc tggtcggcct gatcgcgttc
     2101 ggcggcttcg ccaagggcaa gtggtactga cgcgcgagta agctgcccgg gtggcagcga
     2161 cccgcggtag gcaagtcgaa gaaccgaaac tctcgcgcga ggcgatcctc gacgaggccg
     2221 cgctgctgtt caagcgcaac ggctaccgca agacccggct cgaggaactg gcccaggtgc
     2281 tgggggtgac gcggccggcg atctactacc acttcaaagc caagcaggac ctgcttgtgg
     2341 cgatgtacat gcgctcgatg gaggcgctga acgggatcgc cgccggcagc gaggaggacg
     2401 agggcctgcc gccggtcgag cagttctggc ggcggatcga agcgcacgct atttacatcg
     2461 ccagctaccc ggtcgaggcg gggatcttct tcgaggagga agaggagctg cccgaggacg
     2521 tgcacgcgga agtgcggcgg atgctggtcg cctacaccga tcgcctggtc tccttctaca
     2581 gcgccgccca gaaggccggc gacgcgccgg agggcgatcc gcgggtggcg gtcagcgcga
     2641 tcctcggcgg cgcgatctgg acctaccgct ggtaccccgg gtcggactgg gacgaccccc
     2701 gcgccgcggc gcgggaagtc gtccgtgtcc tccgttcagc gagctagcta gacgcgctcg
     2761 atcagggtgc cggtgccgag cccgccaccg cagcacatcg tgaccaggcc gaggctcttg
     2821 tcagaacgct cgagctcgtg cagcagcgag gtggtcaggc gggcgccggt cgagccgagc
     2881 gggtggccga
//
LOCUS       DASRIG010000123         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_59122, whole genome shotgun sequence.
ACCESSION   DASRIG010000123 DASRIG010000000
VERSION     DASRIG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59122"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VFB52_03355"
     CDS             complement(<1..1092)
                     /locus_tag="VFB52_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05397.1"
                     /translation="MPSTRGFLSVLAVSVCALALVASSAGAATVTNTNDAGPGSLRQA
                     VIDAAPGETITVPAGTYALKSGEILVNKNLTIAGAGQAATIIRAGTDGLRFFNVAKGV
                     TATFTGMTMRDARLTKVSGIAEGGAIYTEEGSATISDVAFVNNVVNASGVATGQEGGI
                     AGGGAIAGENAVVTISASLFSGNQAISRGGSQKGGGIAEGGAAYMEDGQLSITGSTFT
                     GNLVDATAGQGAPTKEQRGGIAGGGAVAAEGDGGLAISASSFSGNRADSRGGPGGPGG
                     IAEGGAVYSEDGLVAIATSAFTNNHVDAAPGQGPANADQDGGIAGGGSVAIDGSLPGS
                     VISAITVTGSSASSAAGPGGSAGIATGGGV"
     gene            complement(1147..2070)
                     /locus_tag="VFB52_03360"
     CDS             complement(1147..2070)
                     /locus_tag="VFB52_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZO05398.1"
                     /translation="MSAAGRPVGLIGVPTSAGAFAPGQEQAPRALRAAGLTERLHRAG
                     AALVDHGDRAVWRWRPDRDEPRAQNLGAVVEIVRDTADRVAAARAAGELTLVLGGDCT
                     VGIGSVAGHSGEGGRPGLLYFDTHADLNVPASVSEGALDWMGMAHMLAEPGTRAELAG
                     AGPRQPLLDPEQVLLFAWGPEQATDHEAAAISRLGLEAFPVERVRASPEEAAAAALAS
                     LEDRCERLLVHFDVDVVDFTDTPLSENWGRNEGLAFEDALRALAVLLRSPALAGLTIT
                     ELNPDHTEEGSDAIARLVGALADGLGGTFAG"
     gene            complement(2067..2435)
                     /locus_tag="VFB52_03365"
     CDS             complement(2067..2435)
                     /locus_tag="VFB52_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO05399.1"
                     /translation="MKVENTDLGKQLGAEKLGARLWRLGPGQASTKHRHFEQEELYVL
                     LEGEGRVRVDEELLTLAPMDTLLVRADSTRQLLNDTEGEQLWLVVGAPPELANTLEMT
                     PEQLARLYPDGPRALPPELQ"
     gene            complement(2508..2918)
                     /locus_tag="VFB52_03370"
     CDS             complement(2508..2918)
                     /locus_tag="VFB52_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO05400.1"
                     /translation="MADSIAQYQSASEAGDIDGIMAALTPDVEVVSPISGRMVFRGSD
                     DVRHLFAAVYGTLKGLRWTRTVGEGPNWFALGEAEVMGVRMTDAMAFELAPDGKIHRI
                     SPHLRPWLALSIFALVLGPKVARKPGVVKRALAG"
     gene            complement(2928..>3746)
                     /locus_tag="VFB52_03375"
     CDS             complement(2928..>3746)
                     /locus_tag="VFB52_03375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05401.1"
                     /translation="IAARRTFWCAALAGIIVFGSLMGAMFIGQQFVQNVLGYTTLGAG
                     AAILPGALGMIVVAPRSAKLVGSHGSRLVLLIGFVFCLLAFTEMLVFWDEDASYLPIG
                     IGYLLVGIGVGFAGTPASRSLTGSVPVHRAGMASGTADLQRDLGGAIMQSIFGALLTA
                     GYTSSFISQINASPEAEKVSEQVQEELTKSFASAATTAEQHPEYAPQIIHAARESFLS
                     GGDETYIAGMAAIALGIAVIFFLFPKKQAETELVERFHAEDTAGAPTATAAATP"
BASE COUNT          519 a         1312 c         1367 g          548 t
ORIGIN      
        1 gacgccgccg ccggtggcga tcccggccga gccgccggga ccggccgccg agctcgcgga
       61 gctgccggtg acggtaatcg ccgagatcac cgacccgggg agcgagccgt cgatcgcaac
      121 gctgccgccc ccggcgatgc cgccgtcctg atcggcgttc gccgggccct gtccgggagc
      181 ggcgtcgacg tggttattgg tgaaggcgga ggtcgcgatc gccaccagtc cgtcttcgga
      241 gtagacggcg ccgccttcgg cgatgccgcc gggcccgccg ggcccgccgc gggaatcggc
      301 gcggttgccg ctgaagctgc tggccgagat cgcgaggccg ccgtcgcctt ccgcggcgac
      361 cgcgccgccg ccggcgatcc cgccgcgctg ttctttggtc ggcgcgccct gccccgcggt
      421 ggcgtcgacc aggttgccgg tgaaggtcga gccggtgatc gagagctgtc cgtcttccat
      481 gtaggcggcc ccgccttcgg cgatcccgcc gcctttttgc gagccgccgc gggagatcgc
      541 ctggttgccg gagaagagcg aggcgctgat cgtcaccacg gcgttctcgc cggcgatcgc
      601 gccgccgccg gcgatcccgc cttcctggcc ggtcgccacg cccgaggcgt tgacgacgtt
      661 gttgacgaag gcgacgtcgc tgatcgtcgc gctgccttcc tcggtgtaga tcgcgccgcc
      721 ttcggcgatt ccagacactt tggtaagacg cgcgtcgcgc atcgtcatgc cggtgaaggt
      781 cgccgtgacg cctttggcga cgttgaagaa gcggaggccg tcggtgccgg cgcggatgat
      841 cgttgccgcc tgaccggcgc cggcgatcgt caggttcttg ttgacgagga tctcgccgct
      901 tttgagcgca taggtcccgg ccgggacggt gatcgtttcg cccggagcgg cgtcgatcac
      961 ggcctggcgc agggagcctg gcccggcgtc gttggtgttg gtgacggtcg cggcgccggc
     1021 gctcgacgcc accagggcga gcgcgcagac ggacacggcc aggacgctga gaaacccccg
     1081 agtgctcggc atgcgaacgg ttctaccagc ccgcgccgac ctcgccaagg ggcttggccg
     1141 agtcctctag cccgcgaacg tcccgccgag gccgtcggcc agtgcaccga ccaagcgcgc
     1201 gatcgcatcc gagccctcct cggtgtgatc ggggttgagc tcggtaatcg tcaggccggc
     1261 gagcgcggga gagcggagca ggacggcgag cgcgcgcagc gcgtcctcga aagcaaggcc
     1321 ctcgttgcgt ccccagttct ccgagagcgg cgtgtcggtg aagtcgacga cgtcgacgtc
     1381 gaagtggaca agcaggcgct cgcagcggtc ttcgagcgag gccagcgccg ccgcggctgc
     1441 ctcctccggg ctcgcgcgca ctcgctcgac cgggaacgcc tcgagcccga gcctggagat
     1501 cgccgcggcc tcgtggtcgg tcgcctgctc gggtccccag gcgaacagca gcacctgctc
     1561 ggggtcgagc agcggttggc gcggtccggc gcccgccagc tccgcccgcg tgccgggctc
     1621 ggcgagcatg tgcgccatcc ccatccagtc gagcgcgccc tcgctgacgc tggccgggac
     1681 gttgaggtcg gcgtgggtgt cgaagtagag gagccccggt cggccaccct caccgctgtg
     1741 gccggcgacg ctgccgatgc cgaccgtgca gtcgccaccc aggaccaggg tcagctcgcc
     1801 cgccgcacgc gcggcggcga cccggtcggc cgtatcgcgg acgatctcga ccacggcacc
     1861 gaggttctgc gctcgcggtt cgtcgcggtc agggcgccag cgccagacgg cacgatcgcc
     1921 gtggtcgacc agcgccgcgc cggcccggtg tagccgctcc gtcagtccgg ccgcgcgcag
     1981 cgcccgcggt gcctgctctt ggccgggcgc gaaggcaccg gcgctggtcg ggacgccgat
     2041 cagaccgacc gggcgtcccg cggcgctcat tgcagctccg gcggcagcgc ccgcggcccg
     2101 tcgggatata gccgcgccag ctgctccggc gtcatctcca gcgtgttcgc cagctccggc
     2161 ggcgccccga ccaccagcca cagctgctcg ccctcggtgt cgttgagcag ctgccgggtc
     2221 gagtcggcgc ggaccagcaa cgtgtccatc ggcgccagcg tcaacagctc ctcgtcaacc
     2281 cgcacccgtc cctcgccctc gagcagcacg tagagctcct cctgctcgaa gtgccggtgc
     2341 ttggtcgaag cctgccccgg ccccagccgc cacaaccgcg cccccagctt ctccgccccc
     2401 aactgcttgc ccagatcggt gttctccacc ttcatctggt tcgaaggccg ccaaaacgcg
     2461 tcaccggctc ggagcaggga gtacgacacg tcccgattat cgcgccgtca gccggcgagg
     2521 gcccgcttca ccacgccggg tttgcgggcg actttggggc cgaggaccag ggcgaagatg
     2581 ctcagggcca gccaggggcg gaggtggggg ctgatgcggt ggatcttgcc gtcgggggcg
     2641 agctcgaagg ccatcgcgtc ggtcatccgg acgcccatca cctcggcctc gccgagggcg
     2701 aaccagttcg ggccctcgcc gacggtgcgg gtccagcgca ggcccttgag ggtgccgtag
     2761 acggcggcga agagatggcg gacgtcgtcg ctgccgcgga agaccatgcg gccggagatc
     2821 ggggagacga cttcgacgtc gggagttagc gccgccatga tcccgtcgat gtcccccgct
     2881 tcagaggcgg actggtactg cgcgatcgag tcggccatgc cgacaggcta cggcgtcgcc
     2941 gcagccgtcg cggtcggcgc gcccgccgtg tcttcggcgt ggaagcgctc gaccagctcg
     3001 gtctcggcct gctttttcgg gaagaggaag aagatcacgg cgatgccgag ggcgatcgcg
     3061 gccatcccgg cgatgtaggt ctcgtcgccg ccgctgagga aggattcgcg ggcggcgtgg
     3121 atgatctggg gcgcgtattc ggggtgttgc tcggccgtgg tcgcggcgct ggcgaaggac
     3181 ttggtcagtt cttcctggac ctgttcggag accttttcgg cttccgggga ggcgttgatc
     3241 tgcgagatga agctggaggt gtagccggcg gtcagcaggg cgccgaagat cgactgcatg
     3301 atcgcgccgc cgaggtcgcg ctggaggtcg gcggtgcccg aggccatgcc ggcgcggtgg
     3361 accgggaccg agccggtcag cgagcgcgag gccggggtgc cggcgaagcc gacgccgatc
     3421 ccgaccagca ggtagccgat cccgatcggc aggtaggagg cgtcctcgtc ccagaagacc
     3481 agcatctcgg tgaaggcgag caggcagaag acgaagccga tcagcaggac caggcgggag
     3541 ccgtgcgagc cgaccagctt ggccgagcgc ggcgcgacca cgatcatccc cagcgccccg
     3601 ggcaagatcg ccgcgccggc cccgagggtg gtgtagccga gcacgttctg gacgaactgc
     3661 tggccgatga acatcgcgcc catcagcgag ccgaagacga tgatcccggc cagcgccgcg
     3721 caccagaagg tgcgacgggc ggcgat
//
LOCUS       DASRIG010000124         8529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_59165, whole genome shotgun sequence.
ACCESSION   DASRIG010000124 DASRIG010000000
VERSION     DASRIG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59165"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1640
                     /locus_tag="VFB52_03380"
     CDS             198..1640
                     /locus_tag="VFB52_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZO05402.1"
                     /translation="MVHRKRGAFALSTFVVAVVMLAAGATAQARSTGAHIFNLTGQPL
                     TIESISTYSSETGAHVWSREDPSVVPPRKGQVLQPGEELHIEIENPVFQTRQVLISFN
                     YYSKAEGWVGFSLALLNGEYPECQGIMGPRQCKIDGGRVEFLEAAGSVHVVDANELQD
                     QARALKTLCTEENECEFEPERWFKAETPRIVYGKTVTNCAEEGGPDVDSTISAKTTVE
                     ISNSVGVDIDVGFKLKVFQAGIKLKYEHERTESEEFKQDVGLHILPERVGWVDITAPV
                     IRDIGTFKLKLGNTEWQIQKVAVDSPDPTRAGNFKADYRKFDAEEFKVECPHERPGEG
                     TGAAPLEGLVTSAALVQTEDKGTNLADLMRGGPESNTLRSRGGDDLVRGGAGGDTLLG
                     GSGRDFIKGGLGEDTIGGGAGPDRIDDVGGPTTVSTGPNSGPGVDRVDVADGRGDDTV
                     RCDTPRTTVFADPRDRVKGPCGQVVRRKGS"
     gene            1732..2121
                     /locus_tag="VFB52_03385"
     CDS             1732..2121
                     /locus_tag="VFB52_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05403.1"
                     /translation="MLTINDRPVFRFGLQRAQAKLISQYMVKEFFQAGNEPRMVEVWD
                     YLVELPGSDGQPKRLTIRVKGFGFDLPEPGNMVPVVVNRRRTKAHFDVDDPTISRSAQ
                     YKRGAQQRKEKAAADKAEFEAKRDWKT"
     gene            2289..2549
                     /locus_tag="VFB52_03390"
     CDS             2289..2549
                     /locus_tag="VFB52_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05404.1"
                     /translation="MAANPKRINVTLDEAHARKLARLAERTHVQEGTLARSLLATALD
                     DADPDASRITEILDAVPGAWERTQEGLAQAGRGEGIPLDELA"
     gene            3213..4349
                     /locus_tag="VFB52_03395"
     CDS             3213..4349
                     /locus_tag="VFB52_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05405.1"
                     /translation="MSGPRWKLAPLLLVLIGLLPLPVSAAAEDSTTTFTDSTSHHHYD
                     VYLGTVDLVRPGAPAPYLRKSAFESEIRFDQEAVEQIGAALPPDSGFSSGISTPAPDG
                     GSWITADPKPGWWEHPGGGAKDQPLFAAADLFAAGMDPAPLLSPGESVSCTDSADQVV
                     LSEVMVGVLSTVAVGPATIWVGEDMSEEFEVVAGSVNINTELSYLNLTVHRLKRTCVV
                     TNAPPPVDRCASGEDKDRDGIADACDEDPSIPDSCALRVARSRVFVYKSKPKVRLVVK
                     YKTRTPAEVKTTYTAKLANGKKLELGSLKQRFKASGTFKLPVSFDEETAAKVRSAKSF
                     AVSFSIPGAPKACARAYAKALTQKQKVGKQTVWFQADAVLAGIF"
     gene            4697..6136
                     /locus_tag="VFB52_03400"
     CDS             4697..6136
                     /locus_tag="VFB52_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZO05406.1"
                     /translation="MLNPPLAYRATLRRFALAASLSLLALAALGAANASAYLYWTESV
                     GSSVNRTTASGAEETRLWVSGAPEMSDVAVDASHIYWSNFYGTIGRANLEGGEVQPEW
                     IKGASYPRGVAVDAGHVYWANFGTNSIGRANLAGGEVEPEWIKGASSPRGLAVDAGHV
                     YWASAGTESIGRANLAGGEVEPEWVPLEIPEGLAVDAGYIYWANVGTNSIGRANLAGG
                     EVEPEFIVGATFPHSVAVDAGHVYWANEGTESIGRANLAGGEVEAEFRKVSVGVSGVF
                     GLAVGNQTADPDPTSLSFGAVVNGSLSAPRSVTFTNDGSQNLIVKGMALGGADPAQFL
                     LEAGGCAATVPPGGSCTVGVRFFPQAAGAFSATLTAQTNAETESVVSLGGTGAPPSLG
                     PPNTSVRRGPAGRSTNRRPRFVFGSNQAGSTFVCRLDKQKFHTCNGKVTFKVKPGHHV
                     VRVKAVGPTGLVDPTAAKRSFVVVAGKKD"
     gene            6264..6599
                     /locus_tag="VFB52_03405"
     CDS             6264..6599
                     /locus_tag="VFB52_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05407.1"
                     /translation="MEPAIGGWKGETAQGFPIFFGVREGRVVTNVRLSYRDAVCGKAS
                     IHKRNVRLSVDESGRFAGIVYPANGGVDLEGSFTGPRRVEGRIVAGEASGLPGCTGGV
                     ISFTAGPKR"
     gene            6797..>8529
                     /locus_tag="VFB52_03410"
     CDS             6797..>8529
                     /locus_tag="VFB52_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO05408.1"
                     /translation="MRSSRGSTLGPRGLSRSILALALLLIALLAPATAQALNFVTSAN
                     GTIWQVNDAAAPAADTGSLWTATGTTASALGGIHVRVSDGTPPLRNGELVRGFGLRYD
                     GGEDFMTTSAVPLGDIAISRQIHVEKGENWTRWIDTFKNMGHQPRLVEVTFGGYVSSG
                     ITAGNQAEVADSSSGDTTIAADDAWVTFATPTAAATTPTSGPSNYGPIAAVFGSPTPF
                     TGGLVGTSQVMLDPFSSPLPASGNGANKVGLREKLVLEPGETKSLVNFLVVGLSETQN
                     LVGAPSIPAAGSEIAAVTATAEELAAEPDFSLLTASQICGISNFDVATLTIPGFAFGE
                     CASVDDPSLAPAPAEPAPKTSSPYDVVGKGVTQLLADMASGETTSQEITRAYLDRIAA
                     YDTGPWGLHSYLHVAGNAMAQARKADEERAAGIAKPLLGVPISLKDLYDTYDQPTTGG
                     FLGLEGYTPPKDGFQVKRLRDAGAVLIGKVNLDELAFGGLGDGSGFGTPWNGLLPSRT
                     AMGSSGGSAASVAASLAAASMGSQTGDSLYGPSTAHSLATIRATTGLTSTQGVIPLFL
                     HGDSAGPIARTV"
BASE COUNT         1508 a         2917 c         2757 g         1347 t
ORIGIN      
        1 gccgaaactt ggtcccgaaa ttggtccccg actccagcga actcagccga actccttgga
       61 caggatcgcg ctcctcagac gggatccgcc tccctggaca ccgtccggct gaacttctaa
      121 tccgacggtc gctggttcga atccagccgg gggcgctata aaatcgccgg cgccaactac
      181 ctaggagctc gttcgggatg gtccatcgca agcgcggcgc attcgccctg tccacgttcg
      241 tcgtcgcggt cgtgatgctg gcggctggcg ctacggccca ggcacgcagc accggggccc
      301 atatcttcaa cctcaccggg caaccgctga cgatcgaaag catcagcacg tactcgtccg
      361 aaacgggcgc ccatgtctgg tcccgggaag accccagcgt cgtcccgcca cgaaagggcc
      421 aagttctgca gccgggcgaa gaacttcata tcgagatcga aaacccggtt ttccagaccc
      481 gccaggtcct gatctcgttc aactactact ccaaagccga aggctgggtc ggcttctcgc
      541 tagctctgtt gaacggcgag tatccggaat gccagggaat catggggccg cggcagtgca
      601 agatcgacgg ggggcgggtc gagttcctcg aagcggcggg gagcgtccac gtcgtcgacg
      661 cgaacgaact ccaggaccag gcgcgggctc taaagactct ctgcacggaa gaaaacgaat
      721 gcgaattcga acccgaacgc tggttcaaag cggaaactcc ccgcatcgtc tacggcaaga
      781 cggtcaccaa ctgcgcggaa gagggcgggc ctgatgtcga ttcgacgatc agcgcgaaga
      841 cgaccgtcga gatctccaac agcgtcggcg tcgacatcga cgtgggcttc aagctcaaag
      901 tcttccaggc gggcatcaag ctcaagtacg agcacgagcg cacggaatcg gaagagttca
      961 aacaagacgt cgggttgcac atcctgcccg aacgggtggg ctgggttgac atcaccgcac
     1021 cggtgattcg cgacatcggc accttcaagc tcaagctcgg aaacaccgaa tggcagatcc
     1081 agaaagtcgc cgtcgacagt cccgatccga ccagggcggg caacttcaag gccgactacc
     1141 ggaaattcga tgccgaagaa ttcaaagtcg aatgcccgca cgaacggccg ggagaaggga
     1201 ccggcgccgc gccgctggaa ggcctggtga cctctgccgc cttggtgcaa accgaagaca
     1261 aaggcaccaa cctcgcggac ctgatgcgcg gcggtcccga gagcaacacc ctccgtagcc
     1321 gaggagggga tgacctggtt cgcggcggtg ccggcggcga cactctgctc ggcggcagcg
     1381 gccgcgactt catcaagggc ggcctcggcg aggacacgat cggcggcggg gccgggcccg
     1441 atcgcatcga tgacgtcggc ggcccgacta cggtcagcac cggacccaac tcgggaccgg
     1501 gtgtcgaccg agtcgacgtc gccgacgggc gcggcgacga cacggtccgc tgcgacacgc
     1561 cgcgaacgac ggtgttcgcc gatccgcgcg atcgagtcaa agggccgtgc ggtcaggtgg
     1621 tccggcggaa aggcagctga gcggcgcggg cgagcgccgc ttcggctcta tcaggccaga
     1681 ttcgccccac tggacgcctt ccggtggagg cccatgtgtt agaaggggat cgtgctgacg
     1741 atcaacgatc gacccgtctt tcgcttcggg ttgcagaggg ctcaggccaa gctcatcagc
     1801 cagtacatgg tcaaggagtt cttccaggcc ggcaacgagc cccgcatggt cgaggtctgg
     1861 gactacctgg tggagttgcc cggatccgac ggtcagccga agcggctgac gatccgagtc
     1921 aaaggcttcg gcttcgacct gcccgagccc ggcaacatgg tcccggtggt ggtgaaccgc
     1981 cggcggacca aggcgcactt cgacgtcgac gacccgacca tctcccgcag cgcgcagtac
     2041 aagcgcggtg cgcagcagcg caaggaaaag gctgccgcgg acaaggcgga gttcgaggcg
     2101 aagcgcgact ggaagacctg agtccggacc ggttgcgcga accgttcgtg gcggaaaact
     2161 tggtcccgaa ttggtccccg actccagcga actcagctga cgatcgctgg ttcgaatcca
     2221 gccgggggcg ttagctggtt gaaggtgctc gaagcacggc ccgtgtatat ctgtgcaatc
     2281 ctggggacgt ggctgccaat ccgaagcgca tcaacgtcac cttggatgaa gcccacgcgc
     2341 gcaagctcgc cagacttgcg gagcgaaccc acgttcaaga gggaacgctc gcgcggtcgc
     2401 tgcttgcgac cgcgcttgac gatgcggacc ccgatgccag ccgcatcacg gagatcctcg
     2461 atgcggttcc cggcgcctgg gagcgcaccc aggaggggct cgcccaggca ggccgcgggg
     2521 aaggcattcc gctcgacgag cttgcctgag tggcgcaggt aatcgtcagc ccgcgagcga
     2581 agcgggacgt ggatgaggcc atctcgagac cgaaccttcc cgctgatacc tggagtcgga
     2641 tcgtccgctc cctgcgggtg cttgaaacgt tcccgctcgc gggtccggag ctgggtggga
     2701 ggtgggcacc gacccgattc gtattgggtc cttggtcgtg gatgctgctg ctctatcgct
     2761 acgacgaggc ttcggatcgt gtcttcgttc tttcggtgaa cgacgctcgt tcagccgcat
     2821 caccgaggtc ttctggtggc tgaaagcttg atcccatgaa gagactggca cggggggtct
     2881 agaccttgcc atcgatcggc gttggtgtgc tgctcttgtt cttcgccgtc atagccggcg
     2941 tagtcgcctg cgtagatgca gtcggcaacg gggagtatct ggcggctctc gcgttcgccg
     3001 tcctcacgct gatcttcgcc gcgggcgtct atctattcct tggggttcgg cgggtgcggc
     3061 aatggtggat gtagcggacc cagcgagccc gagttggccg ttcgccggcc agcgaatcct
     3121 gccgggggca tttcggaatc cgctcccctg gcctgggtga attgcaccca gacctcgtcc
     3181 gtgcctcctc gacccgttgg tagcctgcgc gggtgtctgg cccgcgatgg aagctcgctc
     3241 cgcttctcct cgtccttatc gggctcctcc ctctgcccgt ctcggccgcc gcggaagact
     3301 caacgaccac tttcaccgat tcgacctcgc accaccacta cgacgtctac ctcggcaccg
     3361 tcgacctggt ccggcccggg gcgccggcgc cctatctgcg caagagtgcc ttcgagtcag
     3421 agatccgctt cgaccaggag gcggttgaac agatcggtgc cgcgcttcca cccgattcgg
     3481 gcttctcgtc gggcatttcg acgccggcac ccgatggcgg gtcctggatc accgcggacc
     3541 ccaagccggg ctggtgggaa caccccggtg gcggtgccaa agaccagccg ctgttcgcgg
     3601 cggccgatct gttcgcggcg ggaatggacc cggcgccgct gctctcaccg ggcgagagtg
     3661 tcagctgcac ggactccgct gaccaggtcg tcctcagcga ggtgatggta ggggtcctca
     3721 gcaccgtggc agtcgggccg gcgacgatct gggtcggcga agacatgagc gaagagttcg
     3781 aagtcgttgc gggaagcgtc aatatcaaca ccgagctcag ctacctcaat ctcaccgtcc
     3841 atcgcctgaa gcgcacctgt gtggtcacca acgcgccacc tccggtcgac cgctgcgcct
     3901 cgggcgaaga caaagaccgc gacggcatcg ccgacgcctg tgacgaggac ccctcgattc
     3961 ccgattcatg tgccctgcgt gtcgcccgct cccgcgtctt cgtctacaag tcgaagccga
     4021 aggtccgcct cgtcgtcaag tacaagaccc gcactccggc cgaggtcaag accacctata
     4081 cggccaagct cgccaacggc aagaagctcg agctcggttc cctgaagcag cgcttcaagg
     4141 cgagcggcac cttcaagctg ccggtctcct tcgacgagga gacggcggcc aaggttcgct
     4201 ccgccaaatc cttcgccgtc tccttctcga tccccggcgc acccaaagcc tgcgcccgcg
     4261 cctacgccaa ggcgctgacc caaaagcaga aagtcggcaa gcagaccgtc tggttccagg
     4321 ccgacgcggt gctggccggg atcttctaga ccatcaaatc tatcggcgaa gcgatcgttg
     4381 tcggctgcct acggagccag accgtagagc ccgatcgggt ccccgggcag gagagcctgg
     4441 gtctgcaagc ccgtatcggt cggccacgtg acggtgacgg tcccgggcac gttggtcacc
     4501 tccaccattg gcatgccagt cccttggtgt tcgatcggaa tcgaaacgtt gaagccgaga
     4561 gggctgcccg ggagcagtcc tgaccggcag gcgccgctcc gaaacttggt ccgagcgacg
     4621 gcctttcctc gctatagggt cagaacgctg actactgctg tcacacagcg gccgcccgcc
     4681 gcagagaaga gaaaccttgc tgaacccccc gctcgcctac cgcgccacgt tgcgccgctt
     4741 cgcccttgcc gcgtccctct ccctgctcgc cctggcggcg ctcggcgccg ccaacgcctc
     4801 cgcctacctc tactggacgg aatccgtcgg tagctctgtc aaccggacca cggcaagcgg
     4861 cgccgaagaa acgcgattgt gggtttcggg cgctcccgaa atgagcgacg tagcggtcga
     4921 cgccagtcac atctactggt cgaacttcta cggcacgatc ggccgcgcca acctcgaagg
     4981 cggagaagtt caaccggaat ggatcaaagg cgcgtcttac ccccgcgggg tcgcggtcga
     5041 cgccggccac gtctactggg cgaacttcgg caccaactcg atcggccgcg ccaacctcgc
     5101 cggtggtgaa gtcgaaccgg aatggatcaa aggcgcgtct tccccccgcg gcctcgctgt
     5161 cgacgccggc cacgtctact gggcgagcgc aggaacggaa tcgatcggcc gcgcgaacct
     5221 agccggtggc gaagttgaac cggaatgggt cccgctggaa atcccagaag gcttagccgt
     5281 cgacgccggc tacatctact gggcgaacgt cggcaccaac tcgatcggcc gcgccaacct
     5341 cgccggtggt gaagtcgaac ccgagttcat cgtcggcgcg accttccccc acagcgtcgc
     5401 ggtcgacgcc ggccacgtgt attgggccaa cgagggaacg gaatcgatcg gccgcgccaa
     5461 cctcgccggc ggtgaagtcg aagcggaatt ccgcaaggtc tccgtcggcg tctccggggt
     5521 ctttggactg gcggtcggca accagacagc cgaccccgac cccacttcgc tcagcttcgg
     5581 cgcggtcgtg aacggaagcc tcagcgcacc gcgatcggtc accttcacca acgacggcag
     5641 ccagaacctg atcgtcaaag ggatggcgct gggcggcgcc gaccccgccc agttcctgct
     5701 cgaagccggc ggctgcgccg cgaccgtacc ccccgggggc tcctgcacgg tgggggtccg
     5761 cttcttcccc caggccgcag gtgccttctc ggcgaccctc accgcgcaga ccaatgccga
     5821 aaccgagtcc gtcgtatcgc tcggcggcac cggagcccca ccttcgctcg ggccgcccaa
     5881 cacctcggtc agaagaggcc ccgcgggccg ctccaccaac cgccgtccgc gcttcgtctt
     5941 cggttcgaac caggccggat cgaccttcgt ctgccgcctc gacaagcaga agttccacac
     6001 ctgcaacggc aaggtcacct tcaaagtgaa gcccggccac cacgtagtca gagtcaaagc
     6061 ggtcggcccc accggcctcg tcgatcccac cgccgccaag cgatcattcg tggtcgtggc
     6121 gggaaagaag gactgaggtg ccacggggac cgaaggccgg cattcgtcat cctcggagcg
     6181 gctggtgtct gacggcgatc gcgagcctcc ttctctgcgc gaccgttctg ctcggcgggg
     6241 caccccgggc cggtgcgggt ggggtggagc cggccatcgg cggctggaag ggggaaacgg
     6301 cccagggctt cccgatcttc ttcggggtcc gggaaggcag ggtggtgacc aacgtgcgac
     6361 tgagctatcg ggatgcggtc tgcggcaagg cgagcatcca taaacgcaat gtgaggttga
     6421 gcgtcgacga atcaggcaga tttgccggca tcgtctaccc ggccaacggc ggggtggacc
     6481 tggaaggctc gttcaccgga cctcgacgcg tcgaaggcag gatcgtggcc ggcgaagcat
     6541 ctgggctacc gggctgcacc ggcggggtca tttcgtttac ggcggggcca aagcgctgat
     6601 tgcctggatg acgagcgagc acctcaggct ggagcctcgc ccgtgcgctg gacctccggt
     6661 ctagctgggg aggacacctg gtccttggac ggcgggatag gcgcggtgcc cgaatttcgg
     6721 cccgatggca gttggcggtg gtggggggca ggaattgaat ctctcacccc cacccacaaa
     6781 taggtaagga attcgtgtgc gctcgtcccg cggcagtacg ctcggccctc gcggcctcag
     6841 ccgatccatc ctcgccctcg ccctcctgct gatcgccctg ctggcgcccg ccacggcgca
     6901 ggcgctgaac ttcgtgacca gcgccaacgg aacgatctgg caggtcaacg atgccgccgc
     6961 gccggccgcc gacacgggca gcctctggac ggccaccggc accaccgcga gcgcgctcgg
     7021 cggcattcac gtgcgggtct ccgacggcac gccgccactc cgcaacggcg agctggtccg
     7081 cggcttcggg ctccgctacg acggcgggga ggacttcatg acgacgagtg cggtgccgct
     7141 cggggacatc gcgatctcgc gccagatcca cgtcgagaaa ggtgagaact ggacccgctg
     7201 gatcgacacc ttcaagaaca tggggcacca gccgcgcctg gtcgaagtca cctttggcgg
     7261 ctacgtcagc agcgggatca ccgcgggcaa ccaggcggag gtcgcggaca gctccagcgg
     7321 cgacacgacg atcgccgcgg acgacgcgtg ggtcacgttc gcgacgccca ccgcggcggc
     7381 gacgacgccc acctccggtc cctcgaacta cggcccgatc gcggccgtgt tcggctcccc
     7441 gaccccgttc acgggcgggc tggtcggcac cagccaggtg atgctcgatc ccttctcctc
     7501 gccgctcccg gcgagcggga acggcgccaa caaggtcggc ctgcgcgaga aactcgtgct
     7561 ggagcctggg gagacgaagt cgctggtcaa cttcctcgtg gtcggcctct cggagacgca
     7621 gaacctggtc ggagccccct cgatcccggc cgccggaagc gagatcgccg cggtcacggc
     7681 gacggccgaa gaactggcgg cggaacccga cttctccctg ctgacggcgt cgcagatctg
     7741 cggcatctcc aacttcgacg tcgccaccct gacgattccc ggcttcgcct tcggcgaatg
     7801 cgcgtcggtc gacgacccgt cgctggcccc ggccccggcc gagccggcgc cgaagacctc
     7861 ctcgccgtat gacgtcgtcg gcaagggcgt cacgcagctg ctcgccgaca tggcgtctgg
     7921 agagaccacc tcgcaggaga tcacccgcgc ctatctcgac cggatcgccg cctacgacac
     7981 cgggccgtgg ggcctgcact cctacctcca cgtcgccggc aacgcgatgg cgcaggcccg
     8041 caaagccgac gaggagcggg cggcggggat cgccaagccg ctgctcggcg tgccgatctc
     8101 gctcaaggac ctctacgaca cctacgacca gccgaccacc ggcggcttcc tcggcctcga
     8161 gggctacacg ccgccgaaag acggcttcca ggtcaaacgc ctgcgcgacg ccggcgcggt
     8221 gctgatcggc aaggtcaacc tcgacgagct cgccttcggc ggcctcggcg acggcagcgg
     8281 cttcgggacg ccctggaatg gcctgctgcc ctcgcggacc gcgatgggct ccagcggcgg
     8341 ctcggcggcg agcgtcgccg ccagcctggc ggccgccagc atgggaagcc agaccggcga
     8401 ctcgctctac ggaccctcga ccgcccacag cctggcgacg atccgcgcga ccaccggcct
     8461 gaccagcacc cagggcgtca tcccgctgtt cctccacggc gacagcgccg gtccgatcgc
     8521 ccgcacggt
//
LOCUS       DASRIG010000125         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3458896, whole genome shotgun sequence.
ACCESSION   DASRIG010000125 DASRIG010000000
VERSION     DASRIG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3458896"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFB52_03415"
     CDS             complement(<1..1141)
                     /locus_tag="VFB52_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZO05409.1"
                     /translation="MPDPSIARFRRVITSAYASLEARRQEVNDLNVFPVADGDTGDNM
                     AMTLRAVMEELDRLDGQEVDEIGRTELVQALARAALMGARGNSGVILSQIVRGAAEEL
                     ASRPGELVDPVLVAAAFASAADAAYDSVREPAEGTMLTVFREMAHSLTRQLAHLEADK
                     QRLGRDVGEEQQDAVLAQVLQRAIEDGERAVARTPEQLDVLRESGVVDAGGHGLVLIL
                     AGVVAGLRGEDGELPEVRHHEAPRMSLPHHEDSRYRYCTNFIVAGQNLDSREFLPRLE
                     GLGDSVLVVGDAATLKVHVHTDNPEKAVALFDGIGDVANLDVADMREQMAERSARLEG
                     VTDRTGVVAVAAGEGLERLFGELGANVVPGGETLNPSTYELLAGIH"
     gene            1230..3422
                     /locus_tag="VFB52_03420"
     CDS             1230..3422
                     /locus_tag="VFB52_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05410.1"
                     /translation="MPDFMRSFATWITGHRWTVVISWIVSLVVIGMLASSAGSDFTEE
                     FKLPASDSKEAFDLLETKFPAQSGDTAQIVFKADAGVESSAVQQKMEGIFKQVAKFPH
                     VSEVASPYQEGGAAAVSEDGKIAYATIQYDVASNKIDKEDTREIIAIAEEANGDGLQV
                     ELGGSLITEAEQEEGDPSFAVGLLAAMIILLITFGSVVAMGLPIITALFALGVGISLV
                     GLGTHVFDTANFAPVLAAMIGLGVGVDYALFILTRFRDNLDEGLEPREATINAVNTAG
                     RAVLFAGVTVIIALMGMFLLGLPFLYGVALAAALAVLFTMIAALTLLPALLALVGRRV
                     DRLRIPGLGKRAASTDESGRWYRWSREIQKRPVLAAVLSGAFLLILCFPTLSLRLGTN
                     DAGTEPPDKTTRQAYDLLSEGFGPGFNGPFVMVAALPEKGDAEGLENLSKQLEGEEGV
                     ASVTDINLNEAENTGVFQVYPTTSPQSEATTELLDHIRSDVIPSIEAETGAQLHVGGI
                     NAIFEDFGEAIAEKLPLFIGVVVLLSALLLTIVFRSIWVPLKAVVMNLLSIGAAFGLI
                     VAVFQWGWGASLIGVDGTGPIISFFPIFLFAIIFGLSMDYEVFLMSRIHEEWEHTHDA
                     TESVTRGLALTGRVITAAAAIMITVFASFMLGDDRIIKLFGLGLAAAVLIDAVIIRTI
                     LVPAIMQILGKRAWWFPAWLDWLPKLHVEPEEPPVTGEHPVQPEPARD"
     gene            complement(3565..3756)
                     /gene="rpmB"
                     /locus_tag="VFB52_03425"
     CDS             complement(3565..3756)
                     /gene="rpmB"
                     /locus_tag="VFB52_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZO05411.1"
                     /translation="MAKVCHSCGKGPGFGQSRSHSMVATKRRFEPNLQKVRIIERGSP
                     LRTYVCTRCLKANKVTKAA"
     gene            complement(3809..4303)
                     /gene="ribH"
                     /locus_tag="VFB52_03430"
     CDS             complement(3809..4303)
                     /gene="ribH"
                     /locus_tag="VFB52_03430"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZO05412.1"
                     /translation="MGEGDGQFLETFSDEEKGFLAGQRWAVCVGRFYEDLALRLLAGA
                     DEGFEQLGVPTSSVDRTSVPGAYELPFAAKLFAETGNYAGVVCLGVVIRGETDHFDFV
                     CAESARGIQDVQLQTGVPCAFGVITCDNMDQALARSGGGKRDQGRNGALTVARMALLK
                     RQLS"
     gene            complement(4296..>4496)
                     /locus_tag="VFB52_03435"
     CDS             complement(4296..>4496)
                     /locus_tag="VFB52_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZO05413.1"
                     /translation="KIVGLEGYGLSVSEQVPIESTPNLHNEAYLRTKREKMGHSLHHQ
                     GLPLDEEMIHDEEEHDAETAGG"
BASE COUNT          692 a         1518 c         1522 g          764 t
ORIGIN      
        1 tgtggatccc cgccagcagc tcgtaggtcg aggggttgag cgtctcgccg ccgggcacga
       61 cgttggcgcc gagctcgccg aacaggcgct ccaacccctc acccgccgcg accgcgacga
      121 cgccggtgcg gtcggtcaca ccctcgagac gcgcgctgcg ctcggccatc tgctcgcgca
      181 tgtcggcgac gtcgaggttg gcgacgtcgc cgatcccgtc gaacaaggcc acggccttct
      241 ccgggttgtc ggtgtggacg tggaccttca gcgtggccgc gtcgccgacg accaagaccg
      301 agtcgccgag gccctccaga cggggcagga actcgcggct gtcgaggttc tggccggcga
      361 cgatgaagtt ggtgcagtag cggtagcggc tgtcctcgtg gtggggcagg ctcatccgcg
      421 gcgcctcgtg gtggcggacc tcgggcagct cgccgtcctc gccgcgcagg ccggcgacaa
      481 ccccggcgag gatcagcaca aggccgtggc caccggcgtc gacgaccccg gattcgcgca
      541 gcacgtcgag ctgctcgggg gtgcgtgcca ccgcccgctc gccgtcttcg atcgcccgct
      601 gcaggacctg ggcgaggacc gcgtcctgct gctcttcgcc gacgtcgcgg ccgagccgct
      661 gcttgtcggc ctcgaggtgg gcgagctggc gggtcagcga gtgggccatc tcgcggaaca
      721 cggtcagcat cgtgccctcg gccggctcgc ggaccgagtc gtaggcggcg tcggcggcgc
      781 tggcgaaggc ggcggcgacc aggacggggt cgaccagctc gccggggcga ctggccagct
      841 cctcggcggc gccgcgaacg atctggctga ggatcacacc cgagttgccc cgggcaccca
      901 tcagcgccgc ccgggcgagc gcctggacca gctcggtgcg gccgatctcg tcgacctcct
      961 ggccgtcgag gcggtcgagt tcctccatca ccgcgcgcag ggtcatcgcc atgttgtcgc
     1021 cggtgtcgcc gtcggcgacc gggaagacgt tgaggtcgtt cacctcctgg cgacgcgcct
     1081 ccagggaggc gtaagcagag gtgattaccc gccggaagcg ggcgatcgat ggatcgggca
     1141 tcgaggggaa tctatccggc gaccgcgacg ttcggtaggc gttaactgtc agggaactgt
     1201 caagtagtgg agccgccaac gttgctacgg tgcccgactt catgcgttcc ttcgccacct
     1261 ggatcaccgg ccaccgctgg accgtagtca tcagctggat cgtctccctg gtcgtaatcg
     1321 ggatgctggc cagctccgcc ggttcggact tcaccgaaga attcaagctg cccgcttccg
     1381 actccaagga agccttcgat ctcctggaaa cgaagttccc ggcccagtcg ggcgacaccg
     1441 cgcagatcgt cttcaaggcc gacgccgggg tcgaatcgtc ggcggtccag cagaagatgg
     1501 aagggatctt caagcaggtc gccaagttcc ctcacgtcag cgaagtcgcc agtccctatc
     1561 aggagggcgg cgccgcggcg gtttccgagg acggcaagat cgcctacgcg acgatccagt
     1621 acgacgtcgc cagcaacaag atcgacaaag aggacacgcg cgagataatc gcgatcgccg
     1681 aagaagcgaa cggcgatggc ctccaggtcg agctcggtgg cagtctgatt acggaagccg
     1741 aacaggagga aggcgacccc tccttcgcgg tcggcctgct ggcggcgatg atcatcctcc
     1801 tgatcacctt cggctccgtc gtggcgatgg ggcttcccat catcaccgcc ctgttcgcgc
     1861 tcggggtagg gatcagcctc gtcggcctcg gaacccacgt cttcgacacc gccaacttcg
     1921 cgccggtgct ggcggcgatg atcgggctcg gcgtcggcgt cgactacgcg ctcttcatcc
     1981 tcacccgctt ccgcgacaac ctcgacgagg ggctggagcc acgcgaggcg accatcaacg
     2041 ccgtcaatac cgccggccgc gccgttctat tcgccggggt cacggtgatc atcgccctga
     2101 tggggatgtt cctgctcggt ctccccttct tgtacggagt cgccctggcc gcggccctgg
     2161 cggtcctgtt cacgatgatc gcggcactga cgctgctgcc ggcactgctg gcgctcgtcg
     2221 gccgccgggt agaccggctg cggatcccgg gactcgggaa gcgagcggcc agcaccgacg
     2281 aaagcggccg ctggtaccgc tggagccgtg agatccagaa gcgccctgtc ctagcggctg
     2341 tgctgtccgg cgccttcctc ctgatcctct gcttcccgac tctttcgctg cgcctcggca
     2401 ccaacgacgc cggcaccgaa ccgcccgaca agacgacgcg ccaggcctat gacctgctct
     2461 ccgagggctt cggccccggc ttcaacggtc ccttcgtgat ggtcgcggcg ctgcctgaaa
     2521 aaggcgacgc cgaagggctc gaaaacctga gcaagcagct cgaaggcgaa gaaggcgtcg
     2581 cttcggttac cgacatcaac ctcaacgaag ccgaaaacac gggcgtgttc caggtctacc
     2641 cgacgacctc gccgcagagc gaggcgacga ccgaactgct tgaccacatc cgcagcgacg
     2701 tgattccgtc gatcgaggcc gaaaccgggg cccagctaca cgtcggtggc atcaacgcga
     2761 tcttcgagga cttcggcgag gcgatcgccg aaaagctgcc gctgttcatc ggcgtcgtcg
     2821 tcctgctctc ggccctgctc ctgacgatcg tcttccgctc gatctgggtc ccgctgaagg
     2881 ccgtggtgat gaacctgctc tcgatcggcg ccgccttcgg cctgatcgtc gccgtcttcc
     2941 agtggggctg gggcgccagc ctgatcggcg tcgacggcac cggaccgatc atctccttct
     3001 tcccgatctt cctcttcgcg atcatcttcg ggctctcgat ggactacgag gtcttcctga
     3061 tgtcgcggat ccacgaggag tgggagcaca cgcacgacgc caccgagtcg gtgacccgcg
     3121 gcctcgccct gacggggcgc gtgatcaccg cggcagcggc gatcatgatc accgttttcg
     3181 cctccttcat gctcggcgac gaccggatca tcaaactctt cggcctcggc ctcgcggccg
     3241 ccgtgctgat cgatgccgtg atcatccgca cgatcctcgt cccggcgatc atgcagatcc
     3301 tcggcaaacg cgcctggtgg ttcccagcct ggctcgactg gctacccaag ctgcacgtcg
     3361 agcccgagga accgccggtg accggcgagc atcccgtgca gcccgagcca gcgcgcgact
     3421 agcgccgagc tacggcatct ggagcggcac tcctcgaagg atgggctgag gtcgactgac
     3481 caagccagag tggtgacggc gatgagcgag tagcggagct acttgcgaag aggcggcgcc
     3541 gctatggcgc cgcgtcaggg ggcctcaggc cgccttcgtg accttgttgg cctttaggca
     3601 gcgagtgcac acgtaggtgc gcagggggct gccgcgctcg atgatccgga ccttttgcag
     3661 gttcggctcg aagcggcgct tggtggcgac catcgagtgg cttcgggact ggccgaagcc
     3721 tggtcccttg ccgcagctgt ggcatacctt ggccataagg acggtggatg gtacagggtg
     3781 gtcgggtgcg gcggttttct gcccggggct atgaaagctg gcgcttcagg agggccatgc
     3841 gggcgacggt gagggcgccg ttgcggccct ggtcgcgttt gccgccgccg gaccgggcga
     3901 gggcctggtc catgttgtcg caggtgatca cgccgaaggc gcagggcacg ccggtctgca
     3961 gctggacgtc ctggatgccg cgggccgact cggcgcagac gaagtcgaag tggtcggtct
     4021 ctccgcggat cacgacgccg aggcagacga cgccggcgta gttgccggtc tcggcgaaga
     4081 gcttggcggc gaagggcagc tcgtaggcgc ccgggaccga cgtgcggtcg accgagctgg
     4141 tcggcacgcc gagctgctcg aagccctcgt cggcgccggc cagcaggcgc agggccaggt
     4201 cctcgtagaa gcggccgacg cagaccgccc agcgctgtcc ggcgaggaag cccttctcct
     4261 cgtcgctgaa ggtctccagg aactgcccgt cgccttcacc caccggcggt ctccgcgtcg
     4321 tgttcttcct cgtcgtggat catctcctcg tcgagcggca ggccttggtg gtgcagcgag
     4381 tggcccatct tttcgcgctt ggtccgcagg taggcctcgt tgtggaggtt gggcgtcgat
     4441 tcgatcggca cctgctctga gaccgagagg ccgtagccct cgaggccgac gatctt
//
LOCUS       DASRIG010000126         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1118072, whole genome shotgun sequence.
ACCESSION   DASRIG010000126 DASRIG010000000
VERSION     DASRIG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118072"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..2159
                     /gene="polX"
                     /locus_tag="VFB52_03440"
     CDS             441..2159
                     /gene="polX"
                     /locus_tag="VFB52_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZO05414.1"
                     /translation="MTNAEIAAALDELGVLYELDGAVKYRVLAYGAAAKAIRESPVSV
                     AELAAQGRATELPGVGKTLAEKIDALLETGEIPAAVKLKAKFPPTLIEVTRVPGVGAK
                     TARRLFEELGISTLEELKAAAEAEQIRGVKGLGPKAEQNVLASLERLGEPGEGPGRLL
                     LSVVRPIAEELAAALREHPAANQVEVAGSARRWAETCKDIDLIATAEEPAQLSKALVE
                     HPLIATAGTPGEKGVRVQTHNGISVDLRIVPAAAFGNLLQHFTGSKDHNVQLREDAVG
                     QGFSVSEYGIKDNESGKVTTCESEAAVYKKLGYKYIEPELREGRGELKAARDGKLPKL
                     VELGDLKGDLHSHTTLSDGRNSLEEMAAAAKERGYQYLAITDHSASHGFGDHVTAERL
                     WERIEEVKAFNKGKRGFRLLAGSEINIGLDGSLDYPDDLVAALDWVVASVHTSFSISA
                     AEMTERVVAAIHNPEVNCIGHLTGRLIGRREPYGIDVEAVAEAAAKTGTMIEINGNPN
                     RRDLSDRHARLAADAGVKILLNTDAHGVDTLDNMQYAVATARRAWLTKADIANTLPWA
                     KFKALL"
     gene            complement(2164..2682)
                     /locus_tag="VFB52_03445"
     CDS             complement(2164..2682)
                     /locus_tag="VFB52_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05415.1"
                     /translation="MPFTDLLRVTVFVTGAEATVLGVITALGARREGDDTVVLVAAAW
                     WLIACVIGFWLGRPERAKEDMRDPLARAKTATSLPVETPARIAIGRLWPIVLTAFAAG
                     VLGLFWPGVAVIGAGYALIVSLSWHTREAAVLAIEQRDGVKFYVVPTNALRPVELVRT
                     PGLRSAPRGSAY"
     gene            complement(2686..3501)
                     /gene="otsB"
                     /locus_tag="VFB52_03450"
     CDS             complement(2686..3501)
                     /gene="otsB"
                     /locus_tag="VFB52_03450"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZO05416.1"
                     /translation="MSHEPETGAGPWTTLLEPLRDEPARAAILTDVDGTLAPIAPRAE
                     DAAVLPGTREVLTALSERYGLVGCVSGRQATDARGLVGLDCIAYAGNHGLELLLPGEE
                     TPRLDPSLDGRESQAAEFLAGIDGRLTQSGLRTEDKGPIQALHWRGATDEEAAESRAH
                     EIAIDAGHAGLEPRWGRKVLELRPVGGGGKDAAVASLLAGEQLDRAVYAGDDRTDLDA
                     FARLAELREEGQLSSAVRVGVLSPEGPMELAEEADLTVRGPEEWLAILEWLAE"
     gene            3520..3906
                     /locus_tag="VFB52_03455"
     CDS             3520..3906
                     /locus_tag="VFB52_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZO05417.1"
                     /translation="MTSATGRLSRDELLKLAAIGTLAGLFSGLFGVGGGTVIVPLLIF
                     WFAYGERLATGTSLAAIVFIGLLGAVLQGGIHGNVHVGTGLLLTIPAVLGVVLGTAIQ
                     QRIPQRAVSYLFAVLLVGIAIELILK"
     gene            3903..4271
                     /locus_tag="VFB52_03460"
     CDS             3903..4271
                     /locus_tag="VFB52_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZO05418.1"
                     /translation="MNEVLAVLVCVAGGCAGGLVGVGGGVLFVPALTIFIGLTQVEAE
                     STSLLMIVIVALVGAIRQRGYGNVNLREAAIISVLSPIGVFVGVYLSNELPERALQLS
                     FAALALFMALQLVRRARSAP"
     gene            4304..5173
                     /locus_tag="VFB52_03465"
     CDS             4304..5173
                     /locus_tag="VFB52_03465"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZO05419.1"
                     /translation="MESLKWEGDLPHLRSPVLVCAFRGWNDAAASASTALGAIATALD
                     AEPIAQIDPEEYFDFQTTRPTITLEDGLTRRIEWPENNLLAARVPTADRDLVLLDGTE
                     PNLRWRTFSETVTTAADALDVDLVITLGALIAEVSHTLPVPISGLASSEALVDELELE
                     RSSYEGPTGITGVVHDLCRQAGIDSASLWAAVPHYVAAVPNPKAALALLRRLEGLTGI
                     AVEASELEEETVDYEEQIGRAVAANPEIEELVSRIEAEQVDLLTAEGEELPSADTIAR
                     EFQRFLRQRGKER"
     gene            complement(5180..6283)
                     /locus_tag="VFB52_03470"
     CDS             complement(5180..6283)
                     /locus_tag="VFB52_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZO05420.1"
                     /translation="MRSRLLAIPLAVACLLTLAPTASAAVSWIVHGRGFGHGVGMSAY
                     GAYGYALHGKSYRFILGHYYTGTTVEPLAKTRIVRVLLGTSSGDVEFSGATSACRTKL
                     DPARGYEARRIGNGVRLRTSGGKLVAKCGAKLRAAGAGKITIAGYGTYRGALEASSSG
                     SSLYVVNALALEQYAKGVMPNEVPPSWPTEQLKAQAVAVRSIALTSDNGGSVFDVYPD
                     TRSQVYEGLESEYARTNAAVAATKSQVLLYKGQVATAFYSACSGGHTESVENVFGTAI
                     PYLVGVPDPYDYYCPLHKWTLNFTGPEISERLGAYLDGRLRKVVITKTGASPRIIEAK
                     LVGTGGTTTVSGSELAGALGGHDTWMKFQKVVR"
     gene            6364..>6672
                     /locus_tag="VFB52_03475"
     CDS             6364..>6672
                     /locus_tag="VFB52_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO05421.1"
                     /translation="MASQTVNAVDHKLLAAGEWRQTGAWAEVKSPYDGSLVGRVAQGD
                     AALVNQAIEAADAAFREAEFPQHERAAVLDRAAELVGEQVEDLAQTIAAEAGKPIKTA
                     T"
BASE COUNT         1018 a         2319 c         2344 g          991 t
ORIGIN      
        1 aaagcccgca actcccttga tggacggatc atcgcttaca tggcgttgat agaagccctc
       61 cgacgtaaag ccgtggcggt cttctagctt ctttagggcg cgcgaaagag attgctcgtc
      121 gtagtagttc atcgcgatca ccttcccttt cggtttctta tcgtcgttcc gcagcttcaa
      181 ctaaagcccg gacagatgca cgggcagaga ctctatttac tcgttgaacc tctgtgcacc
      241 tctaaagtta cgcgctttct tgagcagttc caagccctca gagcaaagtt tcaacggggt
      301 ggctcttgat acggcaaacc cagtcgcctc gatcaaacgt ggcgctctga ttcaaactga
      361 cccaccaccc ctgcaaaccg gctgacggtc ttccggaccc taccctggag gccggttctc
      421 agtcaggtta ggttgtcggg atgaccaacg cagagatcgc cgccgcgctc gacgagctgg
      481 gcgtgctcta cgagctcgac ggggcggtca aataccgcgt gctcgcctac ggcgcggccg
      541 ccaaggcgat ccgtgagagc ccggtctcgg tcgccgagct ggccgcgcag gggcgggcga
      601 cggagctgcc gggggtcggc aagacgctgg cggagaagat cgacgcgctg ctggagacgg
      661 gggagatccc ggcggcggtg aagttgaagg cgaagttccc gccgaccctg atcgaggtga
      721 cgcgggtgcc gggggtgggg gcgaaaacgg cgcgccgctt gttcgaggag ctggggatca
      781 gcacgctgga ggagctgaaa gcggcggccg aggccgagca gatccgcggg gtcaaagggc
      841 tcgggccaaa agccgagcag aacgtgctcg cctctctgga gcgcctgggc gagccggggg
      901 aggggccggg acggctgctg ctctccgtgg tccggccgat cgccgaggag ctggcggcgg
      961 cgctgcgcga gcacccggcc gcgaaccagg tcgaggtcgc cggctcggcc cggcgctggg
     1021 ccgagacctg caaggacatc gacctgatcg caaccgccga ggagccggcg cagctctcca
     1081 aggcgctggt cgagcatccg ttgatcgcga ccgccggcac gccgggcgag aaaggcgtgc
     1141 gggtgcagac ccacaacggg atctcggtcg acctgcggat cgtccccgcc gccgccttcg
     1201 gcaacctgct gcagcacttc accggctcca aggaccacaa cgtgcagctg cgcgaagacg
     1261 cggtcggcca gggcttctcg gtctccgagt acgggatcaa ggacaacgag agcggcaagg
     1321 tcacgacctg cgagagcgag gccgcggtct acaagaagct cggctacaaa tacatcgagc
     1381 cggagctgcg tgaggggcgc ggcgagctga aggcggcgcg cgacggcaag ctgccgaagc
     1441 tggtcgagct cggcgacctc aaaggcgacc tgcactcgca cacgaccctc tccgacggcc
     1501 gcaactcgct ggaggagatg gcggcggccg cgaaggagcg cggctaccag tacctggcga
     1561 tcaccgacca ctcggcgagc cacggcttcg gcgaccacgt caccgccgag cggctctggg
     1621 agcggatcga ggaggtcaag gccttcaaca aaggcaaacg cggcttccgc ctccttgccg
     1681 gctcggagat caacatcggt ctcgacggct cgctcgacta ccccgacgac ctggtcgccg
     1741 cgctcgactg ggtggtcgcc agcgtccaca cctcgttctc gatctccgcc gcggagatga
     1801 ccgagcgcgt cgtcgccgcg atccacaacc ccgaggtcaa ctgcatcggc cacctcaccg
     1861 gccgcctgat cggccgccgc gagccttacg ggatcgacgt cgaggcggtc gccgaggcgg
     1921 cggccaagac cggcacgatg atcgagatca acggcaaccc caaccgccgc gacctctccg
     1981 accgccacgc ccgcctcgcc gcggacgccg gcgtgaagat cctgctcaac accgacgccc
     2041 acggcgtcga caccctcgac aacatgcagt acgccgtcgc cacggcgcgg cgcgcctggc
     2101 tgaccaaagc ggacatcgcc aacacgctgc cgtgggcgaa gttcaaagcg ctgctctagc
     2161 gcgtcagtac gcggagccgc ggggggcgct gcgcaggccc ggggtgcgga ccagctcgac
     2221 cggacgcagg gcgttggtcg ggacgacgta gaacttgacg ccgtcgcgct gctcgatcgc
     2281 cagcaccgcc gcctcgcggg tgtgccagga gagcgagacg atcagggcgt agccggcgcc
     2341 gatcacggcg actccgggcc agaacaatcc gagtacgccg gcggcgaagg cggtgaggac
     2401 gatcggccag agccggccga tcgcgatccg ggcgggcgtc tccaccggca gggaggtggc
     2461 ggttttggcg cgggcaaggg ggtcgcgcat gtcctccttg gcccgctccg gccgccccag
     2521 ccagaagccg atcacgcagg cgatcagcca ccaggcggcg gcgacgagga cgacggtgtc
     2581 gtcgccctcg cggcgcgcgc cgagcgcggt gatcaccccg agcacggtcg cctcggcgcc
     2641 ggtgacgaag acggttaccc gcagcaggtc ggtgaacggc accggctatt ccgccagcca
     2701 ctcgaggatc gccagccact cctcgggacc gcgcacggtc aggtcggcct cttcggccag
     2761 ctccatcggc ccctccggcg agagcacccc gacccgcacc gcgctgctca gctggccctc
     2821 ctcgcgcagc tcggcgagcc gggcgaaggc gtcgaggtcg gtgcggtcat cgccggcgta
     2881 gacagcacgg tcgagctgct cgccggccag cagcgaggcg accgccgcgt ccttgccgcc
     2941 accgccgacc gggcgcagct ccaggacctt gcgcccccag cgcggttcga gcccggcgtg
     3001 gccggcgtcg atcgcgatct cgtgggcgcg gctctccgcc gcctcctcgt cggtggcgcc
     3061 gcgccagtgc agcgcctgga tcggtccctt gtcctcggtc cgcagcccgc tctgggtcag
     3121 ccgcccgtcg atcccggcga ggaactcggc cgcctggctc tcgcgcccgt cgagcgaggg
     3181 gtccaggcgc ggcgtctcct cacccggcag cagcagctcg aggccgtggt tgccggcgta
     3241 ggcgatgcag tcgagcccga ccagcccgcg cgcatctgtc gcctggcggc cggagacgca
     3301 gccgaccagg ccgtagcgct cgctcagcgc ggtcagcacc tcgcgcgtcc ccggcagcac
     3361 cgcggcgtcc tcggcgcgcg gcgcgatcgg ggcgagggtg ccgtcgacgt cggtcaggat
     3421 tgccgcccgt gcaggctcgt cccgcagggg ctccagcagc gtggtccaag gacccgctcc
     3481 cgtctcgggt tcgtgagaca cggcaactag tatcgcgaca tgacgagcgc gaccggtcgg
     3541 ctgagccgcg acgaactgct caagctggcc gcgatcggca ccctcgcggg gctcttcagc
     3601 ggtctcttcg gggtcggcgg cgggaccgtg atcgtgcccc tgctgatctt ctggttcgcc
     3661 tacggggagc ggctcgcgac cggcaccagc ctcgccgcga tcgtcttcat cggcctgctc
     3721 ggcgccgtcc tgcagggcgg catccacggc aacgtccacg tcggcaccgg cctgctgctg
     3781 acgatccccg cggtgctcgg cgtcgtgctc ggcacggcga tccagcagcg catcccccag
     3841 cgcgccgtct cctacctctt tgccgtgctc ctggtcggca tcgcgatcga gttgatcctc
     3901 aagtgaacga ggtgctcgcg gtcctcgtct gcgtcgccgg cggctgcgcc ggcggcctcg
     3961 tcggggtcgg cggcggcgtg ctcttcgtgc ccgcgctgac gatcttcatc ggcctcaccc
     4021 aggtcgaggc cgagtcgacc tcgctgctga tgatcgtgat cgtcgccctg gtcggcgcga
     4081 tccgccagcg cggctacggc aacgtcaacc tgcgcgaggc cgcgatcatc tcggtgctct
     4141 cgccgatcgg cgtcttcgtc ggcgtctacc tctccaacga gttgcccgaa cgcgcgctgc
     4201 agctctcctt cgccgcgctc gccctgttca tggcactaca gctggtgcgc cgggcccgct
     4261 ccgccccgta ggggcgcgca tctgcatcgt ctacgctcaa cgggtggaat cactgaagtg
     4321 ggaaggcgac ctgccgcatt tgcggtctcc cgtgttggtc tgcgcttttc gcggctggaa
     4381 cgacgccgcg gcctcggctt cgaccgcgct cggggcgatc gcgaccgcgc tcgacgccga
     4441 gccgatcgcc cagatcgacc ccgaggagta cttcgacttc cagaccacgc ggccgacgat
     4501 cacgctcgaa gacggcctga cgcggcggat cgagtggccg gagaacaacc tgctggcggc
     4561 gcgcgtgccg accgccgacc gcgatctggt gctgctcgac gggaccgagc cgaacctgcg
     4621 ctggcgcacc ttctcggaga cggtgacgac ggccgccgac gcgctcgacg tcgacttggt
     4681 gatcacgctc ggcgcgttga tcgccgaggt ctcgcacacg ctgccggtgc cgatcagcgg
     4741 gctcgcttcg agcgaggcgc tggtcgacga gctggagctg gagcgctcca gctacgaggg
     4801 cccgaccggg atcaccggcg tcgtccacga cctctgccgc caggccggga tcgactccgc
     4861 ctcgctctgg gcggcggtcc cgcactacgt cgccgcggtg ccgaacccga aggcggcact
     4921 ggcgctgctg cgccggctcg agggtctgac cgggatcgcg gtcgaggcct ctgagctgga
     4981 ggaggaaacg gtcgactacg aagagcagat cggccgcgcc gtcgccgcca acccggagat
     5041 cgaggaactg gtctcgcgga tcgaggccga acaggtcgac ctgctcacgg ccgagggcga
     5101 ggagctgccc tccgccgaca cgatcgcgcg cgagttccag cgcttcctgc gccagcgcgg
     5161 caaagagcgc tagcgcccgc tagcggacga ccttctggaa cttcatccag gtgtcgtggc
     5221 cgccgagtgc gccggccagc tccgacccgc tcacggtcgt ggtgccgccg gtcccgacca
     5281 gcttcgcttc gatgatccgc ggtgaggcgc cggtcttggt gatcacgact ttgcgcagcc
     5341 gcccgtcgag gtaggcgccg agccgttcgc tgatctccgg gccggtgaag ttgagcgtcc
     5401 atttgtgcag cgggcagtag tagtcgtagg ggtcggggac gccgaccagg taggggatcg
     5461 cggtgccgaa gacgttctcg acgctctccg tgtggccgcc cgagcaggcc gagtagaagg
     5521 cggtcgcgac ctggcctttg tagaggagga cctggctctt ggtcgcggcg accgcggcgt
     5581 tggtgcgggc gtactcgctt tccagcccct cgtagacctg gctgcgggtg tcggggtaga
     5641 cgtcgaagac gctgccgccg ttgtcgctgg tcagggcgat cgagcggacc gcgaccgcct
     5701 gcgctttcag ctgctcggtc ggccaggagg gcggcacctc gttcggcatc acgcctttcg
     5761 cgtactgctc cagcgcgagc gcgttgacga cgtagagcga gctgcccgag gagcttgcct
     5821 cgagcgcgcc gcggtaggtc ccatagccgg cgatcgtgat tttgccggcg ccggccgcgc
     5881 gcagtttggc cccacatttg gcgacgagtt tgccgccgga ggtgcgcagc cgcaccccgt
     5941 tgccgatccg ccgcgcctcg tagccgcggg ccgggtcgag tttggtccgg caggcgctgg
     6001 tcgcgccgct gaattcgacg tcgccgctgg aggtgccgag cagcacccgc acgatccgcg
     6061 tcttcgccag cggctcgacc gtggtgccgg tgtagtagtg gccgaggatg aagcggtagc
     6121 tcttgccgtg cagcgcgtag ccgtaggcgc cgtaggcgct catcccgacc ccgtggccga
     6181 agccgcggcc gtgcacgatc cagctgaccg ccgcggaggc ggtcggcgcc agcgtcagca
     6241 ggcaggcgac ggcgaggggg atggcgagca ggcgggagcg catctcttga gtccaacaga
     6301 ttcgcgcttg ccaagtcgcg gcctcctcta ggctgggatg aaagcttcga gaggaggtcg
     6361 ggaatggctt cgcaaacggt gaacgcggtt gaccacaagc tgctcgccgc cggcgagtgg
     6421 cgccagaccg gcgcgtgggc agaggtcaag agcccctacg acggcagcct cgtaggccgc
     6481 gtcgcccagg gcgacgcggc cctggtcaac caggcgatcg aggccgccga tgcggcgttc
     6541 cgcgaggccg agttccccca acacgagagg gcggcagtcc tcgaccgcgc ggcggagctg
     6601 gtgggagagc aggtagagga cctggctcag acgatcgcgg ccgaggccgg caagccgatc
     6661 aagaccgcga cg
//
LOCUS       DASRIG010000127         8809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_585328, whole genome shotgun sequence.
ACCESSION   DASRIG010000127 DASRIG010000000
VERSION     DASRIG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585328"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFB52_03480"
     CDS             complement(<1..442)
                     /locus_tag="VFB52_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05422.1"
                     /translation="MEIGIALAVAVALMTNLASLLKHRGCQRTHTISLARPIATIQNL
                     ARSRWFVAGWGLAAVAWLVHIAALSMAPISLVQTVLAGGAVTLAVLSQRLFGDSVERR
                     QWLALILGASGLVLLIATLPHFQGSGSEFSLAAIISFEGGLILLA"
     gene            complement(450..779)
                     /locus_tag="VFB52_03485"
     CDS             complement(450..779)
                     /locus_tag="VFB52_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05423.1"
                     /translation="MALDPVRWDCARCGVSSGQIDGKRIPLPPSWTQAGERSFCLSCS
                     RALAGEAAQDSAPADSSREDLVRIRRRGLIEFEIRRAPEAPNRTIAQACRTSTGAVAA
                     VRDAGED"
     gene            complement(783..1937)
                     /locus_tag="VFB52_03490"
     CDS             complement(783..1937)
                     /locus_tag="VFB52_03490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO05424.1"
                     /translation="MVDALNARSITKPFPIQAMVIRDALDGDDLLVQSPTGSGKTLAF
                     GIPLVDVVEPTKGGPQALVLAPTRELAGQIVDELASLASAQRLRIAAVYGGVGFGPQI
                     AAARRADILVATPGRLEDLIQRGAVTLKEVQVLVLDEADRMLDMGFRPAVDRIVALTP
                     DDRQTLFFSATLAGATGRVAAAYTHDPRSHTHAEPEHAEAKIEHRFLAVDSQSAKLDE
                     LVEQLAGDEAGRSLVFVRTKRGADRLVKRLRSQRIDSVAMHGDKSQGQRERALARFER
                     GDVMTLVATDVAARGIDIPDVARVINYDVPEDREAYVHRIGRTGRAGRTGTGISFVLA
                     DQAEEMHKITSALGLESGLKGSGRPAAGGDKPPQRNRRRRNRRRRTSAAA"
     gene            complement(2145..2351)
                     /locus_tag="VFB52_03495"
     CDS             complement(2145..2351)
                     /locus_tag="VFB52_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05425.1"
                     /translation="MWQVTVVCSACAEEIEVEVENLDDVDREVCECGYGFVVLAVAEF
                     EPVHADQGELIELPRRREIRRRAA"
     gene            complement(2393..2977)
                     /locus_tag="VFB52_03500"
     CDS             complement(2393..2977)
                     /locus_tag="VFB52_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05426.1"
                     /translation="MLSVRGPGNRPPEIPHRGSGVARNLRPHAEGDPWRTPIPDLLPI
                     YLNDHLAGATVGVELARRTAGSNRDDELFGPPLRRICEEIEADRATLEQVMEALGARR
                     DRIKPAAAWIAEKLGRLKPNGQLRGYSPLSRMVELEGLYIGISGKSRLWKALDTTVAA
                     QVPEVDFTALGERADRQRTEVEGLQEEAAKLAFG"
     gene            3094..3831
                     /locus_tag="VFB52_03505"
     CDS             3094..3831
                     /locus_tag="VFB52_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05427.1"
                     /translation="MQSLSKLRQSYIAIPVLVLAAIVLPVAFAGAAGGNSSVSQLKKQ
                     VKTLKRQVATIQEQANSPRPPGGPAGGDLTGVFPNPTIGIDAVATPEIKKDAVTADEI
                     KTGSVGAEEVADNSIAAAEIQDDSIEHTELRQDSVGSEEIVLSGVGSEEIGQRAVGAL
                     EMDAVKTAVSPIGTGISAGQNGSAQVTCPGQTMLIAGGFAWADNEANSIIHSAPKESN
                     PEQSWVVRGFVPSGSNTLYAWATCLAI"
     gene            3844..4659
                     /locus_tag="VFB52_03510"
     CDS             3844..4659
                     /locus_tag="VFB52_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO05428.1"
                     /translation="MTTITDLKQAHRATWAAGDYGAVARHIDDAPPSDLLVRAEVSPG
                     QDVLDVATGTGNVALRAAAAGAQVVGLDLTPELFAEARRRASAAGVTVDWVEGDAEEL
                     PFADASFDRVLSVFGVQFAPRHEVAAAELARVCRSGGRIGLVNWTPSGAIGELFSIMG
                     EYMPAPPDYASPPPLWGSESHVRSLFEGTGVELEFTYGSNPWRFDAAEDWVAFMETRY
                     GPTIKARERLSGEGRWEECRGKVLAMAERRNASSDGSLLLWAEYLVVTGHRAA"
     gene            complement(4759..5100)
                     /locus_tag="VFB52_03515"
     CDS             complement(4759..5100)
                     /locus_tag="VFB52_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO05429.1"
                     /translation="MARASEHKPATRPLPRREAELLAESIAAFSTGSRLRLLWSLLEG
                     ERSVAELAAENELTQAATSQQLRILRDAHLVRVRRDGRSAYYALHDHHVPELLAAMRH
                     HFEHREAGAGS"
     gene            5129..5785
                     /locus_tag="VFB52_03520"
     CDS             5129..5785
                     /locus_tag="VFB52_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZO05430.1"
                     /translation="MHMSPGHPHRHDHSHGGHGHSHGLIDPSIKRSRAGIRAVLLSLL
                     VLGLTALIQVAIFASSGSIALLADLIHNFGDAATAIPLGIAFALRSQRAERGAGYFVV
                     LAIFVSACVAGYEAVDRLLSPEDVSHVGALAAAGVLGFAGNMVAAAIRSRAGRALDSP
                     ALIADGNHARADAYVSLAVVASALAVALGVQIADPLIGLAITAVILKITWDSWWTVRG
                     "
     gene            6186..7037
                     /locus_tag="VFB52_03525"
     CDS             6186..7037
                     /locus_tag="VFB52_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZO05431.1"
                     /translation="MSVSASKPRRPAEMVALEGGIFLMGAEGPECVAADREGPVREVE
                     VEAFSIDRCAVSNRRFAEFAGATGYVTDAERYGWSFVFGGLLPDEFPDTRGAIQAPWW
                     RQVFGACWHRPEGPHSSLDDRADHPVVHVSWRDAEAFCRWSGGRLPTEAEWEFAARGG
                     LVQKRFPWGDDLEPGGEHRMNVWQGTFPAENTLGDGYLGTCPVDAFPANGYGLRNATG
                     NVWEWCADSFDGAEAMVTRGGSYLCHDSYCNRYRVAARSSNTVDTSTGNTGFRCARDP
                     LTNQETR"
     gene            7034..>8809
                     /locus_tag="VFB52_03530"
     CDS             7034..>8809
                     /locus_tag="VFB52_03530"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZO05432.1"
                     /translation="MSSKNDEVKRDVLPIPDRPYEGPITYDAKDPDTSFPPIEPLRPP
                     VEAPNVLVVLLDDVGFAASSAFGGPCATPVAEALAAEGLKYTRFHTTALCSPTRAALL
                     SGRNHHSVGMGGITEIATSAPGYNSVRPNTAAPLAETLKLNGFSTAQFGKCHEVPVWE
                     TSPMGPFDAWPSRGGGFERFYGFIAGETNQYFPALYDDTVAIEPDRSPEEGYHLTEDL
                     ADKAIGWVREQKALMPDKPFFMYFAPGATHAPHHCPEEWSAKYKGRFDEGWDALREVT
                     LARQKELGVVPEDAVLTERPAEIPAWEEMPEELKPVLARQMEIYAGFLEHADHHVGRL
                     IETLRDLEALEDTLIYYVIGDNGASAEGTVNGTFNEAFTFNAANALETPEMLAAKIDE
                     LGTPTANNHFAVGWAHALDTPYKWTKQVGSHWGGTRNGTVVHWPAGIEAKGEIRHQFS
                     HVIDIAATVLDAAGLPEPTFVHGVQQMPLHGKSMLPSFADPDAPEHRETQYFEMFVNR
                     GIYHKGWTAVTRHSVPWMMGTELPPIEDDVWELYAPDDWAQSRDLAAEQPERLAELQR
                     LFLIEAARYNVLPLDDRRVERFNPDL"
BASE COUNT         1330 a         3072 c         3098 g         1309 t
ORIGIN      
        1 cggcgagcag gatcaggcca ccctcgaagc tgatgatcgc cgcgagtgag aactccgagc
       61 cgctgccctg gaagtggggg agggtggcga tgagcaggac aagcccgctg gcgccgagga
      121 tcagcgccag ccactgccgc cgctccaccg agtcgccgaa gaggcgctgg gagagcacgg
      181 cgagcgtcac cgcgccaccg gccagcacgg tctgcacgag cgagatcggc gccatcgaga
      241 gggcggcgat gtggaccagc caggccacgg cggcaaggcc ccagccggcg acgaaccagc
      301 gcgagcgcgc gaggttctgg atcgtcgcga tcggccgggc gagcgagatc gtgtgcgtgc
      361 gctggcagcc gcggtgcttg agcagcgacg ccaggttcgt catcagggcg acggcgacgg
      421 caagcgcgat cccgatctcc acggctgcct cagtcctcgc ccgcgtcgcg gaccgcggcg
      481 acggcaccgg tcgaggtgcg gcaggcctgg gcgatcgtcc ggttcggcgc ttcgggagcg
      541 cgccggatct cgaattcgat caggccgcgg cgccggatcc ggacgaggtc ctcacgcgag
      601 ctgtcggcgg gcgccgagtc ctgcgccgct tcgccggcca gcgcgcggct gcaggagagg
      661 cagaagctcc gctcccccgc ctgagtccag ctcggcggca gggggatgcg cttgccgtcg
      721 atctgtccgg aggagactcc gcatcgcgcg caatcccacc tgaccgggtc gagggccatg
      781 ggctaggcgg cggcgctggt ccggcgccgc cggttacgcc ggcggcgatt gcgctgcgga
      841 ggtttgtcgc ccccggcagc cggcctgcca cttcccttga gcccgctctc cagacccagc
      901 gcggatgtga tcttgtgcat ttcctcggcc tggtcggcga ggacgaagct gatcccggtg
      961 ccggtgcgcc cggcgcggcc ggtgcgcccg atccggtgca cgtaggcctc gcgatcctcg
     1021 gggacgtcgt agttgatcac ccgggccacg tcggggatgt cgatgccgcg ggcggcgacg
     1081 tcggtcgcta ccagggtcat cacgtcgccg cgctcgaagc gcgccagcgc ccgttcgcgc
     1141 tggccctggc tcttgtcgcc gtgcatcgcg accgagtcga ttctctggct gcgcaggcgc
     1201 ttgaccaggc ggtcggcgcc gcgcttggtg cggacgaaga cgagcgagcg cccggcctcg
     1261 tctccggcaa gttgctcgac cagctcgtcg agcttggcgc tctgggagtc gacggcgagg
     1321 aagcggtgct cgatcttcgc ctccgcgtgc tcgggctcgg cgtgggtgtg ggagcgcggg
     1381 tcgtgggtgt aggcggcggc gacgcggccg gtcgcacccg cgagggtggc ggagaagaag
     1441 agggtctggc gatcgtcggg ggtgagggcg acgatccggt cgaccgcggg ccggaacccc
     1501 atgtcgagca tgcggtcggc ctcgtcgagg accaggacct gcacctcctt cagggtgacg
     1561 gctccgcgct ggatcaggtc ctcgagccgc ccgggggtgg cgacgaggat gtcggcgcgg
     1621 cgggctgcgg cgatctgcgg gccgaagccg acgccgccgt agacggccgc gatgcgcagc
     1681 cgctgggcgc tcgccagcga ggcgagctcg tcgacgatct ggccggcgag ctcgcgggtg
     1741 ggggcgagga cgagggcctg tgggccgccc ttggtgggct cgaccacgtc gaccagcggg
     1801 atgccgaaag cgagggtctt gcccgatccg gtcggtgact ggacgagcag gtcgtcgccg
     1861 tcgagggcgt cgcggatgac catcgcctgg atcgggaacg gtttggtgat cgagcgcgcg
     1921 ttgagggcgt cgaccaccgg cttagagatg ccgagatcgg caaaggaagt gatgttgttt
     1981 gctcctgtat tgaaaacgtc tgggggagat cctgggaccc caaggcgcgt cagcacgagg
     2041 aggaaacgga acctctcggg tggcgaattg gccaaacccg acctgaccag cgtagcagtg
     2101 ccttcgcgat cttctttgaa tcgaggggct gcgccgggcc cggctcaggc ggcgcggcgg
     2161 cggatctcgc ggcggcgcgg cagctcgatc agctcgccct ggtcggcgtg aaccggctcg
     2221 aactcggcga cggcgagcac gacgaagccg tagccgcact cgcagacctc gcggtcgacg
     2281 tcatcgaggt tctcgacctc gacctcgatc tcctcggcgc aggccgagca aacgacagtc
     2341 acttgccaca tgacgcaaat ggtcgcgagc cgggcggacg actccgggct cgctacccga
     2401 aagccagctt cgcggcctct tcctgcagcc cctcgacctc ggtccgctgg cggtcggcgc
     2461 gctcgccgag cgcggtgaag tcgacctcgg ggacctgcgc ggcgacggtc gtgtcgagcg
     2521 ccttccagag ccgcgacttg ccgctgatcc cgatgtagag gccttccagc tcgaccatgc
     2581 ggctgagcgg cgagtagccg cgcaactggc cgttcggctt cagtcggccg agcttctcgg
     2641 cgatccaggc ggccgccggt ttgattcggt cgcgcctggc gccgagcgcc tccatcacct
     2701 gctccagcgt cgcccggtcc gcttcgattt cctcgcagat ccgccgtagc ggcggcccga
     2761 acagctcatc gtcccggttg ctgccggcgg ttcgccgcgc cagctcgacg ccgacggtcg
     2821 cgccggcgag gtggtcgttc aggtagatcg ggagcagatc gggaatcgga gtcctccagg
     2881 ggtcgccttc ggcgtgcggt ctgaggttcc gagctacccc gcttccgcgg tggggaatct
     2941 caggtggccg attccccggc ccgcgtacgg acagcaccac cgtcggcggt agaagaccgc
     3001 acccgaagac acgggtcagc ttcataggcg cggggcctcc gcgttgggag attgcgccgg
     3061 tctcattcac tcccagcgaa atggagattc accatgcagt cactcagcaa actcaggcag
     3121 tcctacatcg cgatacccgt tctcgtcctg gccgccatcg tgctgccggt cgcgttcgcg
     3181 ggtgccgcag gaggcaacag ctcggtcagc cagctgaaga agcaggtcaa gaccctcaag
     3241 cggcaggtcg cgacgatcca ggagcaggcc aactccccac ggccgccggg cggcccggcg
     3301 ggcggcgacc tgaccggggt cttcccgaac ccgacgatcg gcatcgatgc cgtcgccacg
     3361 ccggagatca agaaagacgc ggtcaccgcc gacgagatca aaaccggctc cgtcggtgcc
     3421 gaggaggtcg ctgacaactc gatcgccgcg gcggagatcc aggacgacag catcgaacac
     3481 accgagctcc gccaggactc ggtcggctcg gaggagatcg tgctcagtgg ggtcggctcc
     3541 gaagagatcg gccaacgcgc cgttggcgcc ctcgagatgg acgcggtgaa aacggcggtt
     3601 tcgccaatcg ggacggggat cagcgctggt cagaacggca gcgcacaggt cacctgcccg
     3661 gggcagacga tgctgatcgc gggcgggttc gcctgggccg acaacgaggc caactcgatc
     3721 atccactcgg cccccaagga gtccaacccc gaacagtcct gggtcgtccg cggctttgtg
     3781 ccgtcgggca gcaacaccct ctacgcctgg gccacgtgcc tggcgatctg aaaggagacg
     3841 acgatgacca cgatcactga tctcaagcaa gcccaccggg caacctgggc ggcgggcgac
     3901 tatggcgccg tcgcccggca catcgacgac gcgccgccga gcgacctgct cgtccgcgcc
     3961 gaggtgagcc ctggccagga cgtcctcgac gtcgccaccg gcaccggcaa cgtcgccctg
     4021 cgggcggcgg ccgccggcgc ccaggtggtc ggactcgacc tcaccccgga gctgttcgcg
     4081 gaggcgcgcc ggcgggccag cgcggccggc gtcaccgtgg actgggtcga gggcgacgcg
     4141 gaggagctgc cgttcgcgga cgcctccttc gaccgtgtcc tctccgtgtt cggggtgcag
     4201 ttcgcccccc ggcacgaggt cgcggcggcg gagctggctc gcgtttgccg ctccggcggt
     4261 cgcatcggac tcgtcaactg gacgccgtcc ggggcgatcg gcgagctgtt ctcgatcatg
     4321 ggtgaataca tgccggcgcc gccggactac gcctcgccgc cgccgctctg gggcagcgag
     4381 tcccacgtcc gctcgctttt cgaggggacc ggcgtggagc tggaattcac ctacggctca
     4441 aacccctggc ggttcgacgc ggccgaggac tgggtcgcct tcatggagac ccgctacggg
     4501 ccgacgatca aagcccgcga acgtctcagc ggcgaaggtc gctgggagga gtgccgcggc
     4561 aaggtgctcg cgatggccga gcgccgcaac gcgtccagcg acgggtcgct cctcctctgg
     4621 gcggagtacc tggtggtcac cggccacagg gcggcctagc tactcggttc gagcggcccg
     4681 cgtcagtgcg gcgcgggccg ctcgttcatt ttccatccgc aggatggcga ggatgatcat
     4741 cccgatcgtt gctgccggct aggacccggc gcctgcctcg cggtgctcga agtggtggcg
     4801 catcgccgcc agcagctcgg gaacgtggtg gtcgtgcagc gcgtagtagg cgctgcggcc
     4861 gtcgcggcgt acccgcacga ggtgggcgtc gcgcaggatc cgtagctgct gggaggtagc
     4921 ggcctgggtc agctcgttct cggccgccag ttcggcgaca ctgcgctcgc cctccagcaa
     4981 cgaccagagc aggcgcaggc ggctgccggt cgagaaggcg gcgatcgact cggccagcag
     5041 ttccgcctcg cggcggggaa gaggccgggt cgcgggcttg tgctcggagg cgcgtgccac
     5101 cgcggcagga taatagatat gcatatatat gcatatgtct ccaggtcatc cgcatcgcca
     5161 cgaccactcc cacggcggtc acggtcacag ccacggactg atcgacccga gcatcaagcg
     5221 ctcccgtgcc gggatccgcg ccgtcttgct ctcgctcctg gtgctcggcc tcacggcgct
     5281 gatccaggtc gcgatcttcg cctcctcggg cagcatcgcc ctgctcgccg acctgatcca
     5341 caacttcggc gacgccgcca ccgcgatccc gctcggcatc gccttcgccc tgcgctcgca
     5401 gcgggccgag cgcggcgccg gttacttcgt cgtgctggcg atcttcgtct ccgcctgcgt
     5461 cgccggctac gaggcggtgg accggctgct cagccccgaa gacgtgagcc acgtcggtgc
     5521 cctggcggcg gccggcgtgc tcggattcgc cggcaacatg gtcgcggcgg cgatccgctc
     5581 ccgcgccggc cgcgccctcg acagcccggc gctgatcgcc gacggcaacc acgcccgcgc
     5641 cgacgcctac gtcagcctcg ccgtggtcgc cagcgccctc gcggtcgcgc tcggggtgca
     5701 gatcgccgac cctctgatcg gactcgcgat caccgccgtg atcctcaaga tcacctggga
     5761 ctcgtggtgg accgtgcgtg gctagcggcc ggcccgctca gggcgttcgg agcagattga
     5821 gcgacggggg ctggctcaga tcccggcggt ccgatcgtcc tcgccgtaga cgaccaggcc
     5881 gtggatgcag atcgggcagt tcgcgaagat gcgcagcgac cagaacgggt tacccgccgc
     5941 agagctggac gactccgcct tcgtagttgg tgtgaatgca ggctcgcggc cgggcggcgc
     6001 gcgggcatca tctgaaccgg gtgatgtttc ggccgccggg ggctcacccg gttcagatga
     6061 tgcccccgac gcctggcgtg cttagcttcg gtcggatgag cctcgaggac accgggacca
     6121 gttcgtgctg tgccccttcg cgagcggact ccgagtcggg cgccgcccgc aaggcgggcg
     6181 cccacttgtc ggtttcggca tcgaagccgc gccggcccgc cgagatggtg gccctggagg
     6241 gaggcatctt cctgatgggc gccgaggggc cggagtgcgt cgccgccgac cgtgaggggc
     6301 cggtgcgcga ggtcgaggtc gaggccttct cgatcgaccg ctgcgccgtc tcgaaccggc
     6361 gcttcgccga gttcgccggt gcgaccggct acgtgacgga cgccgagcgc tacggatggt
     6421 cgttcgtctt cggcggcctg ctacccgacg agttccccga cacccgcggc gctatccagg
     6481 cgccctggtg gcgccaagtc ttcggtgcct gctggcatcg gcccgaggga ccgcactctt
     6541 cgcttgacga tcgcgccgac caccccgtcg tccacgtgtc ctggcgcgat gccgaggcgt
     6601 tctgtcgctg gagcgggggg aggctgccga ccgaggccga gtgggagttc gcggcccgcg
     6661 gcggcctggt ccagaagcgc ttcccctggg gcgatgacct ggagccgggg ggtgagcacc
     6721 ggatgaacgt ctggcagggc accttcccgg cggagaacac cctcggcgac ggctacctgg
     6781 gcacctgtcc ggtcgatgct ttcccggcca acggctacgg gctccgcaac gcgaccggca
     6841 acgtctggga atggtgcgcg gattccttcg acggcgctga ggcgatggtc acccggggtg
     6901 gctcctatct ctgccacgac tcttattgca accgctaccg ggtcgcggcc cggagctcca
     6961 acacggtgga cacctcaacg ggaaacaccg gattcaggtg cgcccgcgac ccactcacga
     7021 accaggagac gagatgagca gcaagaacga cgaggtcaag cgcgacgtcc tgccgattcc
     7081 cgaccggccc tacgagggtc cgatcaccta cgacgccaag gaccccgaca cgtccttccc
     7141 cccgatcgag cccttgcgcc cgccggtgga ggcgcccaac gtcctcgtcg tcctgctcga
     7201 cgacgtcggc ttcgctgcct ccagcgcctt cggcggaccc tgcgcgaccc cggttgccga
     7261 ggcgttggcc gccgagggcc tgaagtacac ccgcttccac accacggcgc tctgctcgcc
     7321 gacccgcgcc gcgctgctca gcggccgcaa ccaccactcg gtcgggatgg gcgggatcac
     7381 cgagatcgcc acctcggcgc ccggctacaa ctcggtgcgc ccgaacacgg cggcgccgct
     7441 ggccgagacg ctgaagctga acggcttctc gaccgcgcag ttcggcaagt gccacgaggt
     7501 cccggtctgg gagaccagcc cgatgggccc cttcgacgcc tggccctcgc gcggcggcgg
     7561 cttcgagcgc ttctacggtt tcatcgccgg cgagacgaac cagtacttcc cggcgctcta
     7621 tgacgacacc gtcgcgatcg agcccgaccg ctccccggag gagggctacc acctgaccga
     7681 ggacctcgcc gacaaagcga ttggctgggt gcgcgagcag aaggcgctga tgcccgacaa
     7741 gcccttcttc atgtacttcg cccccggtgc cacccacgcg ccgcaccact gccccgagga
     7801 gtggtcggcc aaatacaagg gacgcttcga tgagggctgg gatgcgctgc gcgaggtgac
     7861 gctggcgcgg cagaaggagc tcggcgtcgt ccccgaggac gcggttctga ccgagcgccc
     7921 cgcggagatc cccgcctggg aggagatgcc cgaggagctg aagccggtgc tggcccgcca
     7981 gatggagatc tacgccggct tcctcgagca cgccgaccac cacgtcggcc gcctgatcga
     8041 gaccctgcgt gacctggaag cgctcgagga cacgctgatc tactacgtga tcggcgacaa
     8101 cggggcctcc gcggagggca cggtcaacgg caccttcaac gaggccttca ccttcaacgc
     8161 cgccaacgcg ctggagaccc cggagatgct cgccgccaag atcgacgagc tcggcacccc
     8221 cacggccaac aaccacttcg cggtcggctg ggcgcacgcc ctcgacaccc cctacaaatg
     8281 gaccaagcag gtcggctccc actggggcgg gacgcgcaac ggcacggtcg tccactggcc
     8341 cgccgggatc gaggccaaag gggagatccg ccaccagttc agccatgtga tcgacatcgc
     8401 cgcgacggtt ctcgacgcgg cggggctgcc cgagccgacc ttcgtccacg gcgtccagca
     8461 gatgcccctg cacggcaaga gcatgctgcc gagcttcgcc gaccctgacg cccccgagca
     8521 ccgcgagacg cagtacttcg agatgttcgt caaccgcggt atctaccaca agggctggac
     8581 cgcggtcacc cgccacagcg tcccgtggat gatgggaacc gaactgcccc cgatcgagga
     8641 cgacgtctgg gagctctacg cgccggacga ctgggcgcag tcgcgtgacc tcgccgccga
     8701 gcagccggag aggctggccg agcttcagcg gctgttcctg atcgaggcgg cgcgttacaa
     8761 cgtgctgccg ctcgacgacc ggcgggtcga gcgcttcaac cccgacctc
//
LOCUS       DASRIG010000128         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2397074, whole genome shotgun sequence.
ACCESSION   DASRIG010000128 DASRIG010000000
VERSION     DASRIG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397074"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1196
                     /locus_tag="VFB52_03535"
     CDS             120..1196
                     /locus_tag="VFB52_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05433.1"
                     /translation="MRVARLVLASACLLAAAALVAGCGDDSSSDSTGGAVAVAESRPA
                     PPKADFPAPEGRTLEEVLNLGDKPSLLKIEPAAMVFYEGKNRYPFGIAERDGTEVTDA
                     EVALYFARVPVVKPGARSKSGNKGPVAKAEAGALEQLAIGPFPARIESLAADPEFRAE
                     EAADGRPDPASVVYSTEINFPRNGKWLIAALVKEDDELGGKLLQGAMVGEFKKIPRPG
                     EPAPRIHTPTAQDVGGDPSKISTRIPPDSQNKVDYADALGKEPIVLLFATPQFCQSRV
                     CGPVVDQTEQAKLKYGDQAAFIHMEIYNDNDPADGVRPQVRAFHLPSEPWLFTIDRQG
                     DVSATIEGAFGTELLDEAIEKAIR"
     gene            1193..1558
                     /locus_tag="VFB52_03540"
     CDS             1193..1558
                     /locus_tag="VFB52_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZO05434.1"
                     /translation="MSDKAAPDTELLESFAEEARRRCDVISAGLATATTDFETMRAEA
                     HALRGTAGVVGLRRLAELAGLMESDLAEAKKTGVIRGGRDHQIADAAKALAEGAAAAA
                     KGEEEPPDVGRSLAQLFSA"
     gene            complement(1559..2188)
                     /locus_tag="VFB52_03545"
     CDS             complement(1559..2188)
                     /locus_tag="VFB52_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05435.1"
                     /translation="MFEWARRKGAATALAAAAGGACVLLIAGSGAAGAAAVPGCGAFE
                     AQGDAQDAFMEAGGSPARNAGKMDPDGDGVACEALGAPFKGYATIGYNRKKQFFYGIV
                     KMPAALDGGESPCLYGNKNYPDASRRFNIYKVRTDGDKPLLGRYKAGTQADPAAGTLI
                     WKVERADLVQARYYVAFDERVPLTPYGRNECPGFSSVPTLLPRPQNSGG"
     gene            complement(2250..2323)
                     /locus_tag="VFB52_03550"
     tRNA            complement(2250..2323)
                     /locus_tag="VFB52_03550"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2287..2289),aa:Ile,seq:cat)
     gene            2418..3851
                     /locus_tag="VFB52_03555"
     CDS             2418..3851
                     /locus_tag="VFB52_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05436.1"
                     /translation="MSSKGGSRGGALALVVCALVLLAFAAPASGAIAGSKDRVTTFPQ
                     PRTLPTGGAEELFVDPAGWVWFTQTYEESHGPGTEPSHPPRIVRMNPLGETEIVADHE
                     RAEGFARTADGSVWWTGGFFAINRIAPDGSVHWYPLPETATEYTSPGWQIVAGSDGNV
                     WFGASRMERNPGPEDPQLKAMIVRLTPAGEFTEFALPGRGGYPTRLTLGPDGNVWFTE
                     RNSDRVGKITPSGEVTTFPLPYYARPGDIAVGADGALWFAEEGNETPAIGRITTSGAL
                     SEFPLDVEERSPYSLTAGSDGRIWFALGPATIARISPSGRLSRVQLPHATGPSRLAAG
                     PEGNVWYTAGAGPPCAPGDSGCGDGGYFQAGIIGRVEPAPLSLGIESVKPAKRGTRAK
                     LRVSCFDGMADSVCRGILRLRLGGKEVAKKRFRLGADLSRGLSLKLSRDARERLRSSG
                     RLRLLAQAKLAGGHPVSKRVTLRLPAR"
     gene            complement(3848..>4321)
                     /locus_tag="VFB52_03560"
     CDS             complement(3848..>4321)
                     /locus_tag="VFB52_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO05437.1"
                     /translation="STKATYCSAMRALIEHPDQRQLVLDEPSLVPSTVEEALRMFPAF
                     GHFRRTATRDAELNGTQVKEGDKVVLWYLSSNRDEERYEDPDRFDVTRNPDHQAFGAG
                     GRHFCLGAALARLELKIMLEETLARYPGMELDGRPVFVESAFLNQLKTLPARLAP"
BASE COUNT          694 a         1516 c         1464 g          647 t
ORIGIN      
        1 cgcgcgatcg ctagcccgat gccgccggac cctccggtga tcaacgcagc tctgtccgcc
       61 atcccaactc ctcccgtttc gaagacggag gcagcctacc ggcgaataac atgacgccga
      121 tgcgcgtcgc ccgcctggtc ctcgcttccg cctgcctgct cgcggccgcg gcgctcgtcg
      181 ccggttgcgg cgacgattcg agctccgact cgaccggcgg cgcggtcgcg gttgcggaga
      241 gtcgcccggc gccgccgaaa gcggatttcc ccgcccccga aggccgcacc ctcgaagagg
      301 tgctgaacct cggcgacaag ccgtccctgc tgaagatcga accggcggcg atggtcttct
      361 acgaaggcaa gaaccgctac ccgtttggga tcgccgagcg cgacgggacc gaggtaaccg
      421 acgccgaggt ggcgctctac ttcgcccgcg tgccggtggt caaaccgggc gcgcgttcga
      481 aatcgggcaa caaaggcccg gttgcgaagg cggaagccgg ggctcttgaa cagcttgcga
      541 tcggtccgtt cccggcaagg atcgaaagcc tcgccgccga tcccgagttc cgcgccgagg
      601 aagccgccga cggacggccc gacccggcca gcgtcgtcta ctcgaccgag atcaacttcc
      661 cccgcaacgg caaatggctg atcgcggcgc tggtcaagga agatgacgaa cttggcggca
      721 agctgctgca gggcgccatg gtcggggaat tcaaaaaaat cccgcgtccg ggcgaaccgg
      781 ccccgcggat tcatacgccg accgcgcagg atgtcggggg ggacccctcg aagatctcca
      841 cccggatacc gccggactcc cagaacaagg tggattacgc ggatgcgctc ggaaaagaac
      901 cgatcgtgct cttgtttgcg accccccagt tctgccagag tcgggtctgc ggtccggtgg
      961 tcgaccagac cgagcaggca aagctgaaat acggagatca ggccgctttc atccacatgg
     1021 agatctacaa cgacaacgac ccggccgacg gcgttcgccc acaagtgcga gccttccacc
     1081 tcccgagtga gccgtggctg ttcacgatcg accgccaggg ggacgtcagc gccacgatcg
     1141 agggcgcttt cggtaccgaa cttctcgacg aagcgataga aaaagcgatc agatgagcga
     1201 caaagctgcc cccgacaccg agctgctgga gtccttcgcc gaggaggcgc gtcgccgctg
     1261 cgacgtgatc tcggccgggc tcgccaccgc caccaccgat ttcgagacga tgcgcgcgga
     1321 agcgcacgcc ctgcgcggca ccgccggcgt cgtcggcctg cgccgcctcg ccgagctcgc
     1381 cgggctgatg gagtccgacc tcgccgaagc gaaaaagacc ggcgtgatcc gcggcggccg
     1441 cgaccaccag atcgccgacg ccgccaaggc gctcgccgag ggggccgccg ccgcggcaaa
     1501 gggcgaagaa gagccgcccg acgtcggtcg ctcgctcgcc cagctgttca gcgcctagtt
     1561 acccgcccga gttctgcggc cgcggcagca gcgtcgggac ggagctgaac ccggggcatt
     1621 cgttgcgccc gtagggcgtg agcggcaccc gttcgtcgaa ggcgacgtag tagcgagcct
     1681 ggaccaggtc ggcccgctcg actttccaga tcagcgtgcc cgccgccgga tcggcctgcg
     1741 tgccggcttt gtagcgcccc agcaacggct tgtcgccgtc cgtgcgcacc ttgtagatgt
     1801 tgaaccgccg cgacgcgtcc gggtagttct tgttcccgta caagcagggc gactcgcccc
     1861 cgtccagcgc cgccggcatc ttcacgatcc cgtagaagaa ctgcttcttg cggttgtagc
     1921 cgatcgtcgc gtagcccttg aacggcgccc ccagcgcctc gcaagcaacc ccgtccccgt
     1981 ccggatccat cttgcccgcg ttccgcgccg ggcttccccc ggcctccatg aacgcgtcct
     2041 gcgcgtcccc ctgcgcctcg aacgccccgc acccgggcac cgctgccgca cccgccgcgc
     2101 cgctcccggc gatcagcaat acgcaagcgc cgccggcagc tgccgccagc gccgtcgcgg
     2161 caccctttct cctcgcccac tcaaacatcc gtccaggcat cctagccgcg taaatgcccc
     2221 ggttcccttt gcgctaaacg gcgttgcgat ggggcgggct ggattcgaac cagcgaccaa
     2281 gggattatga gtcccgtgct ctaaccaact gagctaccgc cccaggaccc ggaagatacg
     2341 tggaatcggt gagctgtcgc cgtcgttaca tcagcgccgg cagcaactcc ggcctttctc
     2401 gctggttcaa tgcagcgatg tcgagcaagg gggggagtcg aggcggggcg ctcgcgctgg
     2461 tggtctgcgc gctggtgctg ctcgcgttcg ccgcgccggc gagcggagcg attgccggct
     2521 ccaaggacag ggtcaccacc tttccgcagc cgcgcacctt gccgacggga ggcgcggaag
     2581 agctattcgt cgatcccgcc ggctgggtct ggttcaccca gacctacgaa gaaagccacg
     2641 gccccgggac cgaaccgtcg catccgccgc ggatcgtgcg gatgaacccg ctgggggaga
     2701 ccgagatcgt cgccgaccac gagcgggcgg aagggttcgc acggacggcc gacggcagcg
     2761 tctggtggac gggcggcttc ttcgcgatca accgcattgc tcccgacggg agcgtgcact
     2821 ggtacccgtt gccggaaacg gcgaccgaat acacctcacc cggctggcag atcgtcgccg
     2881 gctccgacgg caacgtctgg ttcggggcgt cccgcatgga acggaacccc gggcccgaag
     2941 acccgcagct gaaagcgatg atcgtgcggc tgacgccggc cggcgagttc accgaattcg
     3001 ccctgcccgg ccgcggcggc tacccgacca ggctcactct cggccctgac ggcaacgtct
     3061 ggttcaccga acgcaacagc gatcgggtcg gcaagatcac tccgagtggc gaggtcacga
     3121 cctttcccct gccgtactac gcccgtcccg gcgacatcgc cgtcggcgcc gatggcgctc
     3181 tctggtttgc cgaggagggc aacgagacgc cggcgatcgg acggatcacg accagcggcg
     3241 cgctgagcga attcccgctc gacgtcgagg aacggtcgcc gtactcgctg accgccggat
     3301 ccgacgggcg gatctggttt gcgctcggcc cggcgaccat cgcccggatc tcgcccagcg
     3361 ggcgcctctc gcgggtgcag ttgccgcacg cgacggggcc gagcaggctc gcggccggtc
     3421 ccgaaggcaa cgtctggtac acggccgggg ccggcccgcc ctgcgcgccc ggggactcgg
     3481 gctgcggcga tggcggctac ttccaggcgg ggatcatcgg ccgggtagaa ccggccccgc
     3541 tctcgctcgg aatcgaaagc gtgaaaccgg ccaagcgcgg cacccgcgcg aagctacggg
     3601 tgagctgctt cgacggcatg gccgactcgg tctgccgcgg catcctgcgg ctgcgcctcg
     3661 gcggcaagga agtcgctaag aagcgcttca ggctgggcgc ggacctctct cgcggcctct
     3721 cgctgaagct gtcccgcgac gcccgcgagc ggctgcggag cagcggtcgt ttgcgcctgc
     3781 tcgcgcaggc gaagctcgcc ggtggccacc ccgtctcgaa gcgtgtgacg ctgcgtctgc
     3841 cggcccgcta gggcgccagc cgcgccggca gcgtcttcag ctggttgagg aaggccgatt
     3901 cgacgaacac cggccggccg tctagctcca tcccggggta gcgggcgagg gtctcctcga
     3961 gcatgatttt cagctcgagt cgggcgaggg cggcgccgag gcagaagtgg cggccgccgg
     4021 cgccgaaggc ctggtggtcg gggttgcggg tgacgtcgaa gcggtcgggg tcctcgtagc
     4081 gctcctcgtc gcggttggag gagaggtacc agaggaccac cttgtcgccc tctttgacct
     4141 gggttccgtt gagctcggcg tcgcgggtgg cggtgcggcg gaagtggccg aaggcgggga
     4201 acatgcgcag ggcctcctcg acggtgctgg ggaccaggga gggttcgtcg agcacgagct
     4261 ggcgctggtc ggggtgctcg atcagcgccc gcatcgcgct gcagtaggtc gccttggtgc
     4321 t
//
LOCUS       DASRIG010000129         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1366535, whole genome shotgun sequence.
ACCESSION   DASRIG010000129 DASRIG010000000
VERSION     DASRIG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366535"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..829)
                     /locus_tag="VFB52_03565"
     CDS             complement(176..829)
                     /locus_tag="VFB52_03565"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZO05438.1"
                     /translation="MTEALLKTPLVGEQPQAQLDRPGRVGFGIGPKGRTLERLVLALE
                     SRAPGTYGHSQRVGQYAGELARALGLSRAESRRVKRAGAIHDVGKTKIPGEILNKPGP
                     LTVQEFVVVSRHAQIGAAMVAGLGDPELTAIVRHHHERFDGSGYPDGLAGEQIPLGSR
                     LIAVCDTFDALTTHRPYREPIGYPEGLAVLEAEAGTQLDPQMVALFCERFAGVWARA"
     gene            990..1640
                     /locus_tag="VFB52_03570"
     CDS             990..1640
                     /locus_tag="VFB52_03570"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZO05439.1"
                     /translation="MRTVILVFDGITALDAIGPYEVLRSVPGWEVQFAGKERGEVRTD
                     SGALGISADHALGEVGEPEIVLVPGGVGNRALMEDDEVLEWLRQVDATSKWTTSVCTG
                     SLVLGAAGLLAGKRSTGHWLFLDPLREFGADPVGGRFVEDGKVITAAGVSAGIDMALH
                     LVGQEVGPEVAQAVQLGIEYDPQPPFDAGHPSKAPVPIVELVTAVSEAEGSSLNLS"
     gene            complement(1650..>3626)
                     /locus_tag="VFB52_03575"
     CDS             complement(1650..>3626)
                     /locus_tag="VFB52_03575"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZO05440.1"
                     /translation="LKAQLQRFCDGINAAAAAAPAPPFEMQLLRLRFEPWEPVDILSL
                     GKLLAFGLSTNWERELVRADMLRELGPELTARLDPTYPADNPVVTQEAWSGDGLAIAG
                     QIDAVRRAMGLAAEASGSNNWAVTGARSETGMPLVAGDPHLPPSMPGIWYEVSLRHGD
                     RFVRGASLPGMPGVYMGQNNDVAWTITNAMGDVQDLFVERVEGDRYQFEDEWLPVESV
                     REEIPVKGREPETLEIRSTHHGPLVNEALGADPEAEPLALRWGTLDEPTAFIGMFELL
                     DIDSGPDLVARLKGHTSPASNMVWADRHGSIGYKLIGRIPRRRGGCPDLPKPGWSGEF
                     EWEGTVPYEELPEVIDPESGYLITANNRIVGDEYPHHITSEYLDGFRAKRIEERLAER
                     ERHSLESFEEIQTDVLSLPGLEAARRLGRLHPPGQRERSAIERLRSWDGQLDSETVAG
                     SIYQAFILRLAREVARSAIGDRDLSERWLDRADNGFTAHSTSPWRWHSHLMTLWEEAD
                     DELVGRPWDGLALEALAGALDDLEERFGPDPEAWRWGRVHEMEFPHPLGEANPLLRKL
                     LNRRLKAGGAQETVSQIAYDPNDPYRAVWAPSWRIVADPCDPDRSRWQMFTGQSGHPA
                     SPHYDDLQADWLEGRTQPMCGEGPWQELELVPPV"
BASE COUNT          486 a         1280 c         1306 g          554 t
ORIGIN      
        1 atccagacct cgccctcgcg gggcacaaac cagagcggca tcgagtgcgg ccagccgcgc
       61 ggcccgaggc tggagacgac ggcgacccgc tcgctctcga gcaactcctc cagctcagcc
      121 tcgctgagca cgatctgatc gcggcggctc acgcgccagc gagcatattg gtccgctagg
      181 cgcgggccca gacgccggcg aagcgctcgc agaacagcgc gaccatctgc gggtcgagct
      241 gtgtgccggc ttcggcctcg agcactgcga ggccctcggg gtagccgatc ggctcccggt
      301 agggccggtg cgtggtcagc gcgtcgaagg tgtcgcagac ggcgatcagc cgggagccga
      361 gcgggatctg ctcgccggcg aggccgtcgg ggtagccgct gccgtcgaag cgctcgtggt
      421 ggtggcggac gatcgcggtc agctccgggt cgccgagccc ggcgaccatc gcggcgccga
      481 tctgggcgtg gcggctgacg acgacgaact cctgcacggt cagcggcccc ggcttgttga
      541 ggatctcgcc cgggatcttg gtcttgccga cgtcgtggat cgccccggcg cgcttgaccc
      601 gccgcgattc cgcccggctc aagccgaggg cgcgggcgag ctcaccggcg tactggccga
      661 cccgctgcga gtggccgtag gtgccgggcg cgcggctctc gagcgccagc accagccgct
      721 ccaaggtgcg ccctttcggc ccgatcccga agccgacccg accggggcgg tcgagctgcg
      781 cttggggctg ttcccccacg agcggcgttt tcagcagtgc ttccgtcatc gaaaccccat
      841 ccagatctcg ggaccggtcg aagtgccttt ccccactaca acagattttg tatcactgct
      901 accaactatt tgtatcggat actcgtttgt ttttgtaagt cttaacctat gccacgcata
      961 cgtctccccg caacgaggag gatggacccg tgcgtaccgt aatcctcgtc ttcgacggaa
     1021 tcaccgcgct cgacgcgatc ggcccttacg aggtgctgcg ctcggtcccc ggctgggagg
     1081 tccagttcgc gggcaaggag cgcggcgagg tccgcaccga cagcggtgcg ctcgggatca
     1141 gcgccgacca cgcgctcggc gaggtcggcg agccggagat cgtgctcgtc cccggcggcg
     1201 tcggcaaccg cgcgctgatg gaggacgacg aagtgctcga gtggctgcgc caggtcgacg
     1261 ccacgagcaa gtggacgacc tcggtctgca ccggctcgct ggtgctcggc gccgccggcc
     1321 tgctcgccgg caagcgctcg accggccact ggctcttcct cgacccgctg cgtgagttcg
     1381 gcgccgaccc ggtcggcggc cgcttcgtcg aggacggcaa ggtgatcacc gccgccggcg
     1441 tctccgccgg gatcgacatg gccctgcacc tggtcggcca ggaggtcggt cccgaggtgg
     1501 cccaggcggt ccagctcggg atcgagtacg acccgcagcc gcccttcgac gccggccacc
     1561 cgagcaaagc cccggtcccg atcgtcgagc tcgtcaccgc ggtctcggaa gccgaaggct
     1621 cctcgctcaa cctcagctga gcccggcgct cagaccggcg ggaccagctc cagctcctgc
     1681 cacggtccct cgccgcacat cggctgcgtg cggccctcca gccagtcggc ctggaggtcg
     1741 tcgtagtggg ggctggcggg gtggccggac tggccggtga acatctgcca gcgtgagcgg
     1801 tcgggatcgc aggggtcggc gacgatccgc cagctcggcg cccagacggc ccggtagggg
     1861 tcgttggggt cgtaggcgat ctggctgacc gtctcctggg cgccgccggc cttgaggcgg
     1921 cggttgagca gcttgcgcag cagcgggttg gcctcgccga gcgggtgcgg gaactccatc
     1981 tcgtgcacgc ggccccagcg ccaggcctcg gggtcggggc cgaagcgctc ctcgaggtcg
     2041 tcgagggcgc cggccagcgc ttccagcgcc agcccgtccc aggggcggcc gaccagctcg
     2101 tcgtcggctt cctcccagag cgtcatcaga tgcgagtgcc agcgccaggg cgaggtcgag
     2161 tgcgcggtga agccgttgtc ggcgcggtcg agccagcgct cggagaggtc gcgatcgccg
     2221 atcgccgatc gcgccacctc gcgcgccagg cgcaggatga aggcttggta gatcgagccg
     2281 gcaacggtct cggagtcgag ctggccgtcc cagctgcgca gccgctcgat cgcgctgcgc
     2341 tcgcgctggc cgggcgggtg caaacggccg agccgccgcg ccgcctccag cccgggcagc
     2401 gagagcacgt cggtctggat ctcctcgaag ctctcgaggc tgtggcgctc acgctcggcc
     2461 aggcgttcct cgatccgctt ggcgcggaag ccgtcgaggt actcgctggt gatgtggtgc
     2521 gggtactcgt cgccgacgat ccggttgttg gcggtgatca ggtagccgct ctcggggtcg
     2581 atcacctccg gcagctcctc gtaggggacc gtgccctccc actcgaactc gccgctccag
     2641 cccggcttcg gcaggtcggg gcagccgccg cggcggcgcg ggatccggcc gatcagcttg
     2701 tagccgatcg agccgtggcg gtcggcccag accatgttcg aggcgggcga ggtgtggccc
     2761 ttcaggcgcg ccaccaggtc gggcccggag tcgatgtcga gcagctcgaa catcccgatg
     2821 aacgcggtcg gctcgtcgag cgtcccccag cgcagggcca acggctcggc ctccgggtcg
     2881 gcgccgagcg cctcgttgac cagcggcccg tggtgggtcg agcggatctc cagcgtctcc
     2941 ggctcgcggc ccttgaccgg gatctcttcg cgcacgctct cgaccggcag ccactcgtct
     3001 tcgaactggt agcggtcgcc ctcgacccgc tcgacgaaga ggtcctggac gtcgcccatc
     3061 gcgttggtga tcgtccaggc gacgtcgttg ttctggccca tgtagacgcc gggcatgccc
     3121 ggcagcgagg cgccgcggac gaagcgatcg ccgtgccgca ggctgacctc gtaccagatg
     3181 cccggcatgc tcgggggcag gtgcgggtca ccggcgacca gcggcatccc ggtctccgag
     3241 cgcgcgccgg tgaccgccca gttgttcgag ccgctggcct cggcggccag ccccatcgcg
     3301 cggcgcacgg cgtcgatctg gccggcgatc gcgagcccgt cgccgctcca cgcttcctgg
     3361 gtgacgaccg ggttgtcggc cggataggtc gggtcgaggc gggcggtcag ctcggggccg
     3421 agctcacgca gcatgtcggc gcggaccagc tcgcgctccc agttggtcga gaggccgaag
     3481 gcgagcagct tgccgaggct gaggatgtcg accggctccc agggctcgaa ccgcagccgc
     3541 agcagctgca tctcgaacgg tggcgccggg gccgccgcgg cggcggcgtt gatcccgtcg
     3601 cagaagcgct gcagctgcgc cttcag
//
LOCUS       DASRIG010000130         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_324616, whole genome shotgun sequence.
ACCESSION   DASRIG010000130 DASRIG010000000
VERSION     DASRIG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324616"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFB52_03580"
     CDS             <1..252
                     /locus_tag="VFB52_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05441.1"
                     /translation="SLINPSLMLIALGRVTPAARGAAMGTYTAFFDAGVGLGAPLAGL
                     AAALSDYEGAFVFAAAVAVASSVLSLLRLRGTHKPVPTP"
     gene            complement(351..1133)
                     /locus_tag="VFB52_03585"
     CDS             complement(351..1133)
                     /locus_tag="VFB52_03585"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZO05442.1"
                     /translation="MPDSFYEPDGERFLATELTRGPWDPGAQHAGPPAALLGRALEQL
                     PGGEGMQIGRVTFEILRSVPIAPVTISAEVVRPGKRVQMLAAELSVEGEVLMRVRGWR
                     LRTSELEIPAEVVSTLPPPHFPDDVKGFEFFPTGHELGYHSAMELHFASGGFMELGPA
                     TAWLRMRYPLVAGEEPSPLQRTLIAADVGNGISAALDFRRFVFVNVDLTVHLERMPTG
                     EWVCVDALTLPQPSGIGTAESVLSDRDGRIGRALQTLLISER"
     gene            complement(1159..1713)
                     /locus_tag="VFB52_03590"
     CDS             complement(1159..1713)
                     /locus_tag="VFB52_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZO05443.1"
                     /translation="MGQKALAGVLVALAAVVVAAPAPAGAATKGLIAPAAACPNQTTR
                     GAPEAVQVKAMLCMVNYARERRGRPPLARLGKLARAARGKAGDILRCDQFDHEACGRE
                     FTYWFKRVDYRGRCTAMGENIAWGTGSLGSVRNIFRAWIGSSGHRANILGNFQEIGIG
                     LRVGTLDGNRGAHVWTQEFGSRTC"
     gene            1792..2187
                     /gene="msrB"
                     /locus_tag="VFB52_03595"
     CDS             1792..2187
                     /gene="msrB"
                     /locus_tag="VFB52_03595"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZO05444.1"
                     /translation="MTQKVQKSEQEWRDQLTPEQYEVTRKAGTERAFTGPHLDEKREG
                     MFRCVGCGTELFSSDTKFDSGTGWPSFTDPANLENVELRFDDSYGMRRTEVVCKTCDA
                     HLGHVFDDGPGASGQRYCINGCALSFDEK"
     gene            complement(2207..2554)
                     /locus_tag="VFB52_03600"
     CDS             complement(2207..2554)
                     /locus_tag="VFB52_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05445.1"
                     /translation="MLHHVGIEVAPEDVGRAVELFELLGFGQVEPPATLSEFTWLERE
                     GTQVHLMPDPSPVAPPRGHLAVVVPEFEATVEALRAAGFEVEAAREHWGAPRARVLAP
                     AGHRVELMAAPPP"
     gene            2635..2886
                     /locus_tag="VFB52_03605"
     CDS             2635..2886
                     /locus_tag="VFB52_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05446.1"
                     /translation="MAVMPRESWTDERLDDFRGEVNRRFDETNRRMDKGFAELREEIH
                     RIHARFDLMDARFDAMQRALMTIGFVLSAALIGMIATQL"
     gene            complement(2883..3806)
                     /locus_tag="VFB52_03610"
     CDS             complement(2883..3806)
                     /locus_tag="VFB52_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZO05447.1"
                     /translation="MGVLLVTAAIAVSTGFGIWAERRWPQGAAEAARKSLVAILYVLL
                     PPVIFFNLAAAEIDLDHGVGLALGLVAQALTATLVWLISSRVLRLPRAQTGAMICTVL
                     VVNTGYLGYPMAAALLGRDELPDAVLWDVLVSGPSLLLGAFAVGAAFGTKAGETPRQR
                     VNAFFTRNPPLYAAIAGLLAPSVLAPDLLVDVSQALVVAILPIGFFAVGATLAEGAEH
                     GELPIPPPLNRQVAVSLVGRLLVAPALLLLLAWPLIELPDAYMLMAVMPCGLNSMVVS
                     HAYGLDNRIVAESIVWSTALVIAAALVSLLI"
BASE COUNT          648 a         1346 c         1431 g          515 t
ORIGIN      
        1 tcgctgatca acccctcgct gatgctgatc gcgctcggcc gcgtcacccc ggcggcgcgc
       61 ggcgcggcga tgggcaccta caccgccttc ttcgacgccg gcgtcggcct cggagccccc
      121 ctcgccggcc tcgccgccgc gctcagcgac tacgagggcg ccttcgtctt cgccgccgcc
      181 gtcgcagtcg cctccagcgt gctcagcctg ctccgcctcc gcggcaccca caagccggtg
      241 ccgacgccct gagagtccgc ccggccgagc agctcaagga cgcagggagc gtggcgtgct
      301 ctgcacgcga gcgaccgagg acgccgagat gcgaagcgcc gggtggactc tcagcgttcg
      361 ctgattagca gcgtctgcaa cgcccggccg atgcggccgt cgcggtcgct cagcaccgac
      421 tcggcggtgc cgatgccgct cggctggggc agggtgaggg cgtcgacgca gacccactcg
      481 ccggtcggca tccgctcgag gtgcacggtg aggtcgacgt tgacgaagac gaagcggcgg
      541 aagtcgagcg cggcgctgat cccgttgccg acgtcggcgg cgatcagggt gcgctgcagc
      601 ggcgagggct cctcgccggc gaccagcggg tagcgcatcc gcagccaggc ggtcgccggg
      661 cccagttcca tgaacccgcc gctggcgaag tgcagctcca tcgccgagtg gtagccgagc
      721 tcgtgcccgg tggggaagaa ctcgaagccc ttgacgtcgt cgggaaagtg cggcgggggc
      781 agcgtcgaga cgacctcggc ggggatctcc agctcggagg tgcgcaggcg ccagccgcgc
      841 acccgcatca gcacctcgcc ctcgacgctg agctcggcgg cgagcatctg gacgcgcttg
      901 cccgggcgaa cgacctcggc gctgatcgtc accggtgcga tcgggaccga gcgcaggatc
      961 tcgaaggtga cgcggccgat ctgcatgccc tcgccgccag gcaactgctc cagggcgcgc
     1021 ccgagcaggg cggcgggcgg accggcgtgc tgggcgccgg ggtcccaggg accgcgggtc
     1081 agctcggtgg cgaggaagcg ctcgccgtcg ggctcgtaga aggagtcggg cacggcggaa
     1141 tcctatggcg ggcggtgctc agcaagtgcg cgagccgaac tcctgcgtcc agacgtgggc
     1201 gccccggttg ccgtcgaggg tgccgacgcg gagcccgatc ccgatctcct ggaaattgcc
     1261 gaggatgttg gcgcggtggc ccgacgagcc gatccaggcc ctgaagatgt tgcgcaccga
     1321 gccgagtgag ccggtgcccc aggcgatgtt ctcgcccatc gccgtgcagc gaccgcggta
     1381 gtcgacccgc ttgaaccagt aggtgaactc gcgcccgcag gcttcgtggt cgaactggtc
     1441 gcagcgcagg atgtcgcccg ccttgccgcg cgcggcgcga gcgagcttcc ccagtcgcgc
     1501 gagcggaggc ctgccgcggc gctcgcgcgc gtagttgacc atgcagagca tcgccttcac
     1561 ctggacggct tccggcgcac cccgggtggt ctggttcggg caggcagcgg ccggtgcgat
     1621 aagacccttc gtcgcagccc cggcgggcgc cggagccgcc acgacgaccg cggccagggc
     1681 gacgagtacg cctgcaagtg ccttctgtcc catctcccaa ggttacggcc cgaaacgggc
     1741 cggccggctt gccgaggggc gtaaaatgga catagagacg aaaggaaaga gatgacccag
     1801 aaggttcaga agtccgagca ggagtggcgc gaccagctga cgccggagca gtacgaggtg
     1861 acccgcaagg cgggcaccga gcgcgccttc accggtccgc acctcgacga gaagcgcgag
     1921 ggcatgttcc gctgcgtcgg ctgtggcacc gagctgttct cctccgacac caagttcgac
     1981 tcgggtaccg gctggcccag cttcacggat ccggccaacc tcgagaacgt cgagctgcgc
     2041 ttcgacgaca gctacgggat gcgccgcacc gaggtcgtct gcaagacctg cgacgcccac
     2101 ctcggccacg tcttcgacga cggtcccggt gcgagcggcc agcgctactg catcaacggc
     2161 tgcgcgctca gcttcgacga gaagtaagcg cagcgggcgc gccgcctcag ggaggcggcg
     2221 ccgccatcag ctccacccgg tgacccgcgg gcgccagcac gcgtgcccgc ggagcgcccc
     2281 agtgctcgcg cgcggcttcg acctcgaagc cggcggcgcg cagtgcctcg accgtcgcct
     2341 cgaactcggg gacgacgacc gccaggtgcc cgcgcggcgg agccaccggg ctcgggtccg
     2401 gcatcaggtg gacctgggtc ccctcgcgct cgagccaggt gaactccgac agcgtcgccg
     2461 gcggctcgac ctgcccgaaa ccgagcagct cgaacagctc gacggcgcgc ccgacatcct
     2521 ccggcgcgac ctcgatcccg acgtggtgga gcatcgcgaa gagcctagag gggcggattg
     2581 agaatttgca aagctgacac ccacgtgtaa cgttttgtct gccagcatgg catcatggcc
     2641 gtgatgccga gagagagttg gaccgacgag cgacttgacg acttccgggg ggaagtgaac
     2701 cggcggttcg acgagacgaa ccgccgaatg gacaaagggt tcgccgaact tcgggaagag
     2761 atccaccgaa tccacgctcg tttcgacctg atggacgcgc ggtttgacgc gatgcagcgc
     2821 gcgctgatga cgatcgggtt tgttctctcc gcggccttga tcggcatgat cgccacccag
     2881 ctctagatca gcagcgagac gagcgcggcg gcgatcacca gggcggtcga ccagacgatc
     2941 gactcggcga cgatccggtt gtcgagaccg taggcgtggg agacgaccat cgagttgagg
     3001 ccgcagggca tcaccgccat cagcatgtag gcgtcgggaa gctcgatcag cggccaggcg
     3061 agcaggagca gcagcgccgg ggcgaccagg aggcggccga cgaggctgac cgccacctgc
     3121 cggttgagcg gcggcgggat cggcagctcg ccgtgctcgg cgccctcggc gagcgtcgcc
     3181 cccaccgcga agaacccgat cggcaggatc gccacgacca gggcctgcga gacgtcgacg
     3241 aggaggtcgg gggcgagcac gctgggggcc agcaggccgg cgatcgccgc gtagagcggc
     3301 gggttgcggg tgaagaacgc gttcacccgc tggcgtgggg tttcgcccgc cttggtgccg
     3361 aacgccgcgc cgaccgcgaa ggcgccgagc agcagcgagg ggccgctgac cagcacgtcc
     3421 cagagcaccg cgtccgggag ctcgtcgcgg ccgagcagtg cggccgccat cgggtagccg
     3481 aggtagccgg tgttgacgac cagcacggtg cagatcatcg ccccggtctg agcccgcggc
     3541 agccgcagga cccggctcga gatcagccag acgagggtcg cggtgagcgc ctgggcgacg
     3601 aggccgagag ccaggccgac gccgtggtcg aggtcgatct cggccgccgc gaggttgaag
     3661 aagatgaccg gcggcaggag cacgtagagg attgccacca gcgatttgcg ggccgcctcg
     3721 gcagcaccct gcggccagcg gcgctcggcc cagatgccga acccggtcga gactgcgatt
     3781 gccgcggtga ccagtaggac acccatctat gcggcgccct tcgccgtgcc cgcgtccatc
     3841 tacagggcga cctagagggc cgagtcccta gaaggggagc gaggagccgg cgcgttcggc
     3901 gaactcgacg agcggctggg ggatcacgcc gaagaagacg
//
LOCUS       DASRIG010000131         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3719289, whole genome shotgun sequence.
ACCESSION   DASRIG010000131 DASRIG010000000
VERSION     DASRIG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719289"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFB52_03615"
     CDS             <1..946
                     /locus_tag="VFB52_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZO05448.1"
                     /translation="LLGHAATGVLTALRRLGRWLRRALDHPGRARRRAADGLAGLWEV
                     TWNLARPAPKTPLNTPIGAGRSFCWTEFELADLKQIKNAFGGTVNDVSLAVVAGALRE
                     WLRERGEPVEGVELKALVPVSIRTEDEHGELGNKLTAMRGPLPVGTADPVARLREISA
                     AMDRLKDSKQPLGAEAIWGLNDWFRDFAPPVLLAPTAAVNFSTRLFNLLVTNFPGPQV
                     PFYVLGRELTGIYPVGFLARKHALAIAILSYNGRVGFGVLADPAALPDAERLVGHLER
                     ALEELRAPAQIASPANGDELAGDSRPRFARDRERLGRG"
     gene            876..1886
                     /gene="ftsY"
                     /locus_tag="VFB52_03620"
     CDS             876..1886
                     /gene="ftsY"
                     /locus_tag="VFB52_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZO05449.1"
                     /translation="MATSWQEIVDLGSRATANDSAAAEPEPEPGKRNRGMFRRLRESL
                     SKSRQALQEEIGASLFDRIDEETWEALEEALILADAGAPTTAAVVERLEHEVEAGTVP
                     ADGVAVRDRLIEILAEVASTGRAPIDLSAEPAVLMMVGVNGTGKTTTIGKIAWHLSKE
                     MGLTVVMAAGDTYRAAAAEQLAEWADRAGAELVRGESGADPGAVAYDAINAARARNAD
                     VVIVDTAGRLHTQDNLMEELAKVRRVIGKQIPEAPHETLISIDATTGQNGLRQAKVFS
                     ESVPVNGVVLTKLDGTAKGGIALAIASELEIPVKLIGVGETLEDLRPFDPTEYAQALL
                     AE"
     gene            1935..>3537
                     /locus_tag="VFB52_03625"
     CDS             1935..>3537
                     /locus_tag="VFB52_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZO05450.1"
                     /translation="MRSALLAFCLALALAAVAVAATPAIKCRAATCKGTPRADRIVGS
                     SKPNRIVAGGGRDTVLARQGDDRLLGGKGADHVEGGVGADLLDGGSGVDELLGGEGAD
                     LLLGGAANDTISGGLGNDSADGGEGNDVVKGVWGEDALDGGAGNDQVLGGEENDTLAG
                     GLGDDVLDGALGDDRLLGGPGADLFKGGMGIDTADYTPVGTPVGVTLDDLANDGGDGD
                     NVKPAVENVIGGSGDDTLIGSAEENWFWGGPGADRIDGGFGIDHLDGDEGPDQLVGGE
                     ESDRLYGGPGDDRFDGGKGADRMFGEGGDDHFEGGNAEDRFVGGAGADSFDGGSGPDT
                     VDYTAETAAVTVTIDELADDAGEGDDVGLSVENLATGPGADDLSGSALPNTFNAGPGT
                     DLLWGEAGADVLFGEAGDDSLHGGADGDTLEGGEGNDTLLGEEGSDWIYGGEGDDLLD
                     GGLGFNVLAGEDGDDTLAGGPGNERLEGGAGNDTIGAGGGADVVGGGPGSDLIDIEDG
                     VGDDVACGDSIGSGGEGVDTILLDPG"
BASE COUNT          545 a         1300 c         1275 g          417 t
ORIGIN      
        1 gctgctcggt cacgccgcca ccggcgtgct gaccgccctg cgccgcctcg gccgctggct
       61 gcgccgcgcc ctcgaccacc ccggtcgcgc ccgccgccgc gccgccgacg gcctcgccgg
      121 tctctgggag gtgacctgga acctcgcccg cccggccccg aaaacgccgc tcaacacgcc
      181 gatcggggcg ggccggagct tctgctggac ggagttcgag ctggccgacc tgaagcagat
      241 caagaacgcc ttcggcggca ccgtcaacga cgtctcgctg gcggtcgtcg ccggcgccct
      301 gcgcgagtgg ctgcgcgaac gcggcgagcc ggtcgaggga gtcgagctga aggcgctggt
      361 cccggtctcg atccgcaccg aggacgagca cggcgagctc ggcaacaaac tgaccgcgat
      421 gcgcgggccg ttgccggtcg gcaccgccga cccggtcgcg cggctgcgcg agatcagcgc
      481 cgcgatggac cggctgaagg actccaagca gccgctcggg gcggaagcga tctggggcct
      541 caacgactgg ttccgggact tcgcgccccc ggtcctgctg gcgccgaccg cggcggtcaa
      601 cttctcgacc cgcctgttca acctcctggt caccaacttc cccggcccgc aggtcccgtt
      661 ctacgtgctc ggccgcgagc tgaccgggat ctacccggtc gggttcctcg cccgcaagca
      721 cgcgctcgcg atcgcgatcc tcagctacaa cggtcgggtc ggcttcggcg tgctcgccga
      781 tcccgccgcg ctgcccgacg ccgagcgcct cgtcggccac ctcgaacgtg ccctggagga
      841 gctccgggcg cccgcgcaga tagcctcccc ggccaatggc gacgagctgg caggagatag
      901 tcgacctcgg ttcgcgcgcg accgcgaacg actcggccgc ggctgagccc gagcccgagc
      961 ccgggaaaag aaatcgcggc atgttccgcc gcctgcgcga gagcctctcg aaaagccgtc
     1021 aggcgctgca ggaggagatc ggcgccagcc tcttcgaccg gatcgacgag gagacctggg
     1081 aggcgctgga ggaggcgctg atcctcgccg acgccggcgc cccgaccacc gccgcggtgg
     1141 tcgagcggct cgagcatgag gtcgaggccg ggaccgtgcc cgccgacggc gtcgccgtgc
     1201 gcgaccgcct gatcgagatc ctcgccgagg tcgcctcgac cggccgcgcc ccgatcgacc
     1261 tctccgccga gcccgcggtg ctgatgatgg tcggcgtcaa cgggaccggc aagacgacga
     1321 cgatcggcaa gatcgcctgg cacctctcca aagaaatggg cctcacggtg gtgatggccg
     1381 ccggcgacac ctaccgcgcc gctgccgccg agcagctcgc cgagtgggcc gaccgcgccg
     1441 gagcggagct ggtccgcggc gaatccggcg ccgaccccgg tgccgtcgcc tacgacgcga
     1501 tcaacgccgc tcgcgcccgc aacgccgacg tcgtaatcgt cgacaccgcc ggccgcctcc
     1561 acacccagga caacctgatg gaggagctgg ccaaggtgcg gcgggtgatc ggcaagcaga
     1621 tccccgaggc tccgcacgag accctgatct cgatcgacgc gaccaccggc cagaacgggc
     1681 tgcgccaggc gaaggtcttc tccgaatcgg tgccggtcaa cggcgtcgtc ctgaccaagc
     1741 tcgacggcac cgcgaagggc gggatcgcgc tcgcgatcgc ctccgagctg gagatcccgg
     1801 tgaagctgat cggggtcggc gagacgctcg aggacctgcg cccgttcgac ccgaccgagt
     1861 acgcccaggc gctgctcgcc gagtaaccac tcgctaacgc cagacacccg gcgcccgcgt
     1921 acggtcgcgg catcgtgcgc tcggccctgc tcgcgttctg cctcgccctg gcgcttgccg
     1981 ccgttgccgt ggcggcgacc ccggcgatca agtgccgcgc cgcgacctgc aaaggcaccc
     2041 cgcgcgccga ccgcatcgtc ggctcctcga agccgaacag gatcgtcgcg gggggcggcc
     2101 gcgacaccgt gctcgcccgc cagggcgacg atcgcctgct cggcggcaag ggcgccgacc
     2161 acgtcgaggg cggggtgggg gcggacctgc tcgacggcgg ttccggcgtc gacgagctgc
     2221 tcggcggcga aggcgccgac ctcctgctcg gcggcgccgc caacgacacg atctccggcg
     2281 gtctcggcaa cgacagcgcc gacggcggcg agggcaacga cgtggtcaag ggcgtctggg
     2341 gtgaggacgc gctcgacggc ggagccggca acgaccaggt gctcggcggc gaagaaaacg
     2401 acaccctggc cggtgggctc ggcgacgacg tgctcgacgg cgcgctcggc gacgatcgcc
     2461 tgctcggcgg ccccggcgcg gacctgttca aaggcgggat ggggatcgac accgccgact
     2521 acacgccggt cggcaccccg gtcggggtca cgctcgacga cctcgcaaac gacggcggcg
     2581 acggcgacaa cgtcaagccc gcggtcgaga acgtgatcgg cgggtcgggt gacgacaccc
     2641 tgatcggctc cgccgaggag aactggttct ggggcggccc cggggccgac cggatcgacg
     2701 ggggcttcgg gatcgaccac ctcgacggcg acgagggccc cgaccagctg gtcggcggcg
     2761 aagaatcgga tcgtctctac ggcggccccg gcgacgaccg cttcgacggg ggcaagggcg
     2821 ccgaccggat gtttggcgaa ggcggcgacg accatttcga aggcggcaac gccgaagacc
     2881 gcttcgtcgg cggtgccggg gccgacagct tcgacggcgg cagcggtccc gacacggtcg
     2941 attacacggc cgagaccgcg gcggtgacgg tgacgatcga cgagctcgcc gacgacgccg
     3001 gggagggcga cgacgtcggc ctctcggtcg agaacctggc caccggcccc ggcgccgacg
     3061 acctctccgg ctccgcgctc cccaacacct tcaacgccgg tccgggcacc gacctgctct
     3121 ggggcgaagc cggtgctgac gtcctcttcg gcgaagccgg cgacgacagc ctgcacggcg
     3181 gcgccgacgg cgacacgctc gaaggcggcg aaggcaacga caccttgctg ggcgaggaag
     3241 gcagcgactg gatctacggc ggcgaaggcg acgacctgct cgacggcggc ctcggcttca
     3301 acgtgctggc cggcgaagac ggcgacgaca cgctcgcagg cggccccggc aacgagcggc
     3361 tcgaaggcgg cgccggcaac gacacgatcg gcgccggcgg cggtgccgac gtggtcggcg
     3421 gcggtcccgg cagcgacctg atcgacatcg aagacggggt cggcgacgac gtcgcctgcg
     3481 gcgacagcat cggcagcggg ggcgaaggcg tcgacacgat cctgctcgac cccggcg
//
LOCUS       DASRIG010000132         4435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2135766, whole genome shotgun sequence.
ACCESSION   DASRIG010000132 DASRIG010000000
VERSION     DASRIG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135766"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFB52_03630"
     CDS             <1..190
                     /locus_tag="VFB52_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05451.1"
                     /translation="LCDRLAIIDAGKIVREGTPAALKADLRERRGLDSDPTLDDVFLD
                     ATGRSRDRVAGDVQEVSA"
     gene            187..942
                     /locus_tag="VFB52_03635"
     CDS             187..942
                     /locus_tag="VFB52_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05452.1"
                     /translation="MTQAWLLGQRALREAWRTPDALLPTLFIPLFFLVVNTGQAAKIF
                     PSDSTDFLQGQSYGAFQLPITLLLAASFGMAALFLVEEIEGGYFEKLRAMPIPRYSLV
                     LGRLCSEAVKGIVISTLIVIVALPFGITINSGPLGFVLLIALSALWGVVYAGFMQLIA
                     LKTRSAAATNSGGLIFFPLLFLTPNFVPREMLAAPMEVAATINPVTYLMEALRSLILD
                     DLDWGTVSLGFAVVGGLGAVMLALTVRMIRSYD"
     gene            complement(860..2089)
                     /locus_tag="VFB52_03640"
     CDS             complement(860..2089)
                     /locus_tag="VFB52_03640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO05453.1"
                     /translation="MSKQSFADLGVSKAVLSALSKRGIEQPFAVQRLVIEDVLAGRDV
                     LAQSPTGSGKTLAFGVPLIDRIAAESKRPAGLVLAPTRELACQIVDELYEIAHARALS
                     VAPVYGGVGIQAQARKAARAHIVVATPGRLEDLLQRGDLTLKHVQVLVLDEADRMLDM
                     GFKPAVDRIVAKVPADRQTLFFSATLDGAPGEIARSYTRDARRHVHAPKAERRADVQH
                     RFVHLAHEDKVGALVRELDETERGRTLVFVRTKRGADRLVKRIGKQGIKATAMHGDKS
                     QSQRRRALADFEAGKIDTLIATDVAARGIDVPEVTHVFNYDAPEDRDTYVHRIGRTGR
                     AGATGAGVSFVIADQVGDVRKIAEGLGLRSEFEAGAPARTGPSKHRSAASRSSGSSAP
                     SGQASRPRARRRRRSRG"
     gene            complement(2219..3505)
                     /locus_tag="VFB52_03645"
     CDS             complement(2219..3505)
                     /locus_tag="VFB52_03645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZO05454.1"
                     /translation="MVGAIASGKRLKDRYRLERTLGRGGMAAVWLGHDERLDRPVAVK
                     VLSDTISSDPGFVARFRREAQTAAGLSHPNLVQVYDFSDEGERPYLVMEFVPGETLAA
                     HLERGDRVDCDQLARELLEALAHIHEVGILHRDVKPSNIVVEPDGTARLIDFGIALPR
                     DATALTRTGLVLGTERYTAPEVMEGQMATERSDLYSCGVVLGACKGRSSRALRELVAA
                     LVSRDPRDRPLSAEHALELLDWQDGVEEQATEVLEQPAASDSGSNRALVAGAICAVLG
                     IAAIVVALVLSSGGSDPATGGNQQAAQVKDGQAEERPASAAPATEQPAEAAAPDPATA
                     DPATGAALNDQGYAMVQAGEYEAAVPVLEEAVAAFPAGTDDINYAYALYNLGNALRLS
                     GRPEEAIPILEQRLGIPDQEAAVRAELDRAIADLQG"
     gene            complement(3550..4215)
                     /locus_tag="VFB52_03650"
     CDS             complement(3550..4215)
                     /locus_tag="VFB52_03650"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05455.1"
                     /translation="MATKAGTEKPLRRDAEENRQRLLAAAREVFAEQGLEATMDDVAE
                     RAGVGVGTAYRRFANKEELIDALFGERIGELAVIMERANAAEDPWQGIVTYLEETVAL
                     SAGDKGLREVMLSSPHGHDFVDAARERLEPQIETLLARAHGAGVLRPGIESTDLVMIV
                     LMLAQISDYGHDRDDPPWRRFLTLALDGIRPTSAGALPGQALSPEEQADLLQGAAVTR
                     RGR"
BASE COUNT          590 a         1537 c         1600 g          708 t
ORIGIN      
        1 cctgtgcgac cggctggcca tcatcgacgc cggcaagatc gtccgcgagg ggacgcccgc
       61 cgccctcaag gccgacctgc gcgagcggcg cggcctcgac agcgatccca ccctcgacga
      121 cgtgttcctc gacgcgaccg gccgctcccg tgaccgcgtc gccggcgacg tgcaggaggt
      181 gtcggcatga cccaggcctg gctgctcgga cagcgcgcgc tgcgcgaggc gtggcggacg
      241 cccgacgccc tcctccccac gctgttcatc ccgctcttct tcctggtcgt caacaccggc
      301 caggcggcga agatcttccc ctcggactcg accgacttcc tccaggggca gagctacggc
      361 gccttccagc tgccgatcac gctgctgctc gcggcctcgt tcgggatggc ggccctgttc
      421 ctggtcgagg aaatcgaggg cggctacttc gagaagctgc gggcgatgcc gatcccccgc
      481 tactcgctgg tcctggggcg gctctgctca gaggcggtca agggcatcgt gatctcgacc
      541 ctgatcgtga tcgtcgcgct gcccttcggg atcacgatca acagcgggcc gctgggcttc
      601 gtcctgctga tcgcgctctc ggccctctgg ggcgtggtct acgccggctt catgcagctg
      661 atcgcgctga agacgcgctc cgccgcggcg acgaactcgg gcgggttgat cttcttcccg
      721 ctgctcttcc tcaccccgaa cttcgtcccc cgcgaaatgc tcgcggcgcc gatggaggtc
      781 gcggcgacga tcaaccctgt cacctacctg atggaggccc tgcgctcgct gatcctcgac
      841 gacctcgact gggggacggt tagcctcggc ttcgccgtcg tcggcgggct cggggccgtg
      901 atgcttgccc tgacggtgcg gatgatccgg agctacgact agcggccgag cgatgcttgc
      961 tggggccggt gcgggcgggc gcaccggcct cgaactcgct gcgcaggccg aggccctcgg
     1021 cgatcttgcg cacgtcgccg acctggtcgg cgatcacgaa gctgaccccg gccccggtgg
     1081 cgccggcgcg gccggtgcgg ccgatccggt gcacgtaggt gtcgcggtcc tcgggcgcgt
     1141 cgtagttgaa gacgtgggtg acctcgggga cgtcgatccc acgggcggcg acgtcggtcg
     1201 cgatcagggt gtcgatcttg ccggcttcga agtcggcgag ggcgcggcgg cgctgggact
     1261 gcgacttgtc gccgtgcatc gccgtcgcct tgatcccctg cttgccgatc cgcttgacca
     1321 ggcggtcggc gccgcgcttg gtgcggacga agacgagggt gcggccgcgc tcggtctcgt
     1381 cgagctcgcg gaccagggcc ccgaccttgt cctcgtgggc gaggtggacg aagcggtgct
     1441 ggacgtcggc gcggcgctcg gccttcggcg cgtgcacgtg gcggcgggcg tcgcgggtgt
     1501 aggaacgggc gatctcgccg ggggcgccgt ccagcgtcgc cgagaagaac agcgtctggc
     1561 ggtcggcggg gaccttggcg acgatccggt cgacggcggg cttgaagccc atgtcgagca
     1621 tccggtcggc ctcgtcgagg accagcacct ggacgtgctt gagggtgagg tcgccgcgct
     1681 ggagcagatc ctcgagccgg ccgggggtgg cgacgacgat gtgggcgcgg gcggccttgc
     1741 gggcctgcgc ctggatcccg acgccgccgt agacgggggc gacggagagc gcccgggcgt
     1801 gggcgatctc gtagagctcg tcgacgatct ggcaggcgag ctcgcgggtc ggcgccagca
     1861 cgaggccggc cggccgcttc gactcggccg cgatgcggtc gatcagcggc acgccgaagg
     1921 cgagcgtctt gcccgacccg gtcggcgact gggcgaggac gtcacggccg gcgaggacgt
     1981 cctcgatgac caggcgctgg acggcaaacg gctgctcgat cccgcgcttg ctgagcgcgg
     2041 acagcacggc tttggacacg ccgagatcgg cgaatgactg ctttgacatg aactgcgact
     2101 cctttttgat gcgacaggga gatccaacga ccctggacgc gtgagacgca gcaaaggacg
     2161 ggttctctcg gctacccggg tggcaaccga cgccggggac cgtagcagca aaggccgatt
     2221 acccctgcaa atcagcgatc gctctgtcca actccgcgcg cacggccgct tcctggtcgg
     2281 ggatcccgag ccgctgttcg aggatcggga tcgcctcctc gggtcggccg ctgaggcgca
     2341 gggcgttgcc gaggttgtag agcgcgtagg cgtagttaat gtcgtcggtg ccggccggga
     2401 aggcggcgac cgcttcttcg agcaccggca ccgcggcctc gtattcgccg gcctggacca
     2461 tcgcgtagcc ctggtcgttg agcgccgcgc cggtggccgg gtcggccgtg gccgggtccg
     2521 gagcggcggc ttcggccggt tgctcggtcg cgggcgcggc agacgccggg cgttcctcgg
     2581 cttggccgtc tttcacctgg gctgcctgct ggttgccacc cgtcgcggga tcgctcccgc
     2641 cgctcgacag caccagggcg acgacgatcg cggcgattcc gagcacggcg cagatcgcac
     2701 ccgcaaccag cgcgcggttg ctcccggaat ccgaggcggc cggctgttcg agcacttcgg
     2761 tcgcctgctc ttcgacgccg tcctgccaat cgagcagttc gagcgcgtgc tcggccgaga
     2821 gcgggcggtc acgcgggtca cggctgacca gcgccgcgac cagctcgcgc agcgcccgcg
     2881 aggagcggcc cttgcaggcg cccagcacga cgccgcagga gtagaggtcc gagcgctcgg
     2941 tcgccatctg gccctccatc acctcgggcg cggtgtagcg ctcggtgccg agcaccaggc
     3001 cggtccgggt cagcgcggtc gcgtcacgcg gcagggcgat cccgaagtcg atcagccgcg
     3061 cggtcccgtc gggttcgacg acgatgttcg acggctttac gtcgcggtgc aggatcccga
     3121 cctcgtggat gtgggcgagc gcttccagca gctcccgcgc gagctggtcg cagtcgaccc
     3181 ggtcgccgcg ctccaggtgg gcggccagcg tctcccccgg cacgaactcc atcaccaggt
     3241 agggccgctc gccctcgtcg gagaagtcgt agacctggac caggttcggg tgggagaggc
     3301 ccgccgccgt ctgcgcctcg cgccggaagc gggcgacgaa accggggtcg gaggagatcg
     3361 tgtcggagag gaccttgacc gcgaccggcc ggtccagccg ttcgtcgtgc ccgagccaga
     3421 ccgccgccat gccgccgcgg ccgagcgtgc gctcgagccg gtagcggtcc ttcagccgct
     3481 ttccgctggc gatcgcgccg accaccgcat tcccctaccc acttggcggg gaatcatcgg
     3541 cggcgcagct cagcgcccgc ggcgggtgac ggcggcgccc tgcagcagat cggcctgttc
     3601 ctctgggctc agcgcctggc cgggcagggc cccggcggaa gtagggcgga tgccgtcgag
     3661 cgccagcgtg aggaagcgac gccagggcgg gtcgtcgcgg tcgtggccgt agtcggagat
     3721 ctgggcgagc atcagcacga tcatcaccag atcggtcgac tcgatcccgg ggcggagcac
     3781 gccggcgccg tgggcgcggg cgagcagggt ttcgatctgc ggctcgagcc gctcgcgggc
     3841 cgcgtcgacg aagtcgtgcc cgtgcgggga gctcagcatc acctcgcgca ggcccttgtc
     3901 gccggcgctc agcgcgaccg tctcttccag gtaggtgacg atcccctgcc agggatcctc
     3961 ggcggcgttg gcccgctcca tgatcacggc gagctcgccg atccgctcgc cgaacagcgc
     4021 gtcgatcagc tcctccttgt tggcgaagcg ccggtaggcg gtgccgaccc cgaccccggc
     4081 gcgctcggcg acgtcgtcca tcgtcgcctc cagcccctgc tcggcgaaga cctcgcgggc
     4141 ggcggcgagc aggcgctgcc ggttctcctc ggcgtcgcgg cggagcggct tctcggtgcc
     4201 tgccttcgtt gccatgcgga caatctatca aagcggaggc atgatctccg tttatgtgct
     4261 accgtgatcg aaacggaggt gctctctccg cttacaccct cttctcaatc gcctatctga
     4321 aagtgagatt cccgatgcaa gcccacgaca acccggatca ccgcaaacgc tgggggatcc
     4381 tcgccatcct ggggctggcg cagctgatgg tcgtgctcga cacgacgatc gtgaa
//
LOCUS       DASRIG010000133         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3181258, whole genome shotgun sequence.
ACCESSION   DASRIG010000133 DASRIG010000000
VERSION     DASRIG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3181258"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFB52_03655"
     CDS             <1..908
                     /locus_tag="VFB52_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZO05456.1"
                     /translation="IAGNPSFDLGERPELALVLGLAVALTILVNVVVLQWRFRPLERL
                     VDEMERTDLSRPRANLRPAVDGRSDPQEVVRLELAFRRMLDRLETERRRISSAALAAQ
                     EEERARVARDLHDEVNQSLTGLLLRLEAAREAAPPELETELEETKALANQAMRELLSL
                     ARQLRPTALDDLGLAAAVAGQVEQLGRGEIEASFAAEGDFSDLADDAQLVVYRVAQEA
                     LSNAARHSGAARVDVRMQRDPDGGVGLEVADDGRGFAFDEAEKGLGIAGMRERALLVG
                     GELTIESRPEHGTTVRLALAGESAP"
     gene            905..1534
                     /locus_tag="VFB52_03660"
     CDS             905..1534
                     /locus_tag="VFB52_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05457.1"
                     /translation="MKVLIADDHGIVRSGLRLLLERQEDIEVIGEAADGAEARDLAVR
                     ERPDLAILDVKMPKLTGLQATREIKKQAPEVSVLILSMHDDERYLFEALKAGASGYVL
                     KAQADTDLLAAIRAVERGEPFLTPEAQRTLIKDVLERGSSGEEELTPREEEIVKLVAE
                     AHTTKQIAELLHLSEKTVENHRANAMRKLGMRDRVELVRYAIRRGLIEP"
     gene            1608..2078
                     /locus_tag="VFB52_03665"
     CDS             1608..2078
                     /locus_tag="VFB52_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZO05458.1"
                     /translation="MLFSTKAEYGVRLMVELGRQPGSQPISLNTVAEAERLPLSYLEH
                     LVAKLRKAGLVTSTRGAHGGYRLALPAEQITMDEVVQALEGQIAPMECFHETPEGKVL
                     CSHEDDGDRACSTKLLWTRVQGGVTKALAGTTLAELVEFSRPGGRTEADIASAA"
     gene            2115..>2792
                     /gene="sufC"
                     /locus_tag="VFB52_03670"
     CDS             2115..>2792
                     /gene="sufC"
                     /locus_tag="VFB52_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZO05459.1"
                     /translation="MAELEIRNLHVEVEDKKILKGLDLDVEKGKVHALMGPNGSGKST
                     LANVIMGHPALEVTEGTIIFKGEDITEASPEDRSQAGLFMAFQYPVAIPGVSVSKYLR
                     MIINAQREARGEDQIKIKDFAKTAKEAMELANIPNDFSSRYLNDGFSGGEKKRMELLQ
                     LALLRPEIAVLDETDSGLDIDALRVVAEGVNKFAGPDMGVLIITHYQRILHLVKPDSV
                     HVMFEGRI"
BASE COUNT          485 a          967 c          959 g          381 t
ORIGIN      
        1 gaattgccgg caatcccagc ttcgacctcg gcgagcggcc cgagctggcg ctggtgctcg
       61 ggctggccgt tgccctgacg atcctcgtca acgtggtggt gctccagtgg cggttccgcc
      121 ccctcgagcg gctggtggac gagatggagc gaaccgacct ctcgcgcccc cgcgccaacc
      181 tgcggccggc ggtcgacgga cgttccgacc cgcaggaggt ggtcaggctc gagctcgcgt
      241 ttcgccgcat gctcgaccgc ttggagaccg agcgccggcg aatctccagc gccgcgctcg
      301 cggcccagga ggaggagcgg gctcgcgtag cccgcgacct ccacgacgag gtcaaccagt
      361 cgctgaccgg gctgctctta cggctggagg cggcacgcga agccgcgccc ccggagctgg
      421 agaccgagct ggaagagacg aaagcgctgg ccaaccaggc gatgcgcgag ctgctctccc
      481 tcgcccgcca gctgcgtccg accgcgctcg acgacctcgg cctcgccgcc gcggtagcgg
      541 gccaggtcga gcagctcggc cgcggcgaga tcgaggcctc cttcgcggcc gagggcgact
      601 tctccgacct cgccgacgac gcccagctgg tcgtctaccg ggtcgcccag gaggcgctct
      661 ccaacgccgc ccgccacagc ggcgccgccc gcgtcgacgt gcggatgcaa cgcgaccccg
      721 acggcggggt cgggctcgag gtcgccgacg acggccgcgg cttcgccttc gacgaggccg
      781 agaagggcct cgggatcgcc ggcatgcgcg agcgcgcgct cctggtcggc ggtgagctga
      841 cgatcgagtc gcgtcccgag cacgggacca cggtgcgact ggcgctggcg ggagaatcgg
      901 cgccgtgaag gtgctgatcg ccgacgacca cggaatcgtc cgctccgggc tgcggctgct
      961 gctcgagcgc caggaggaca tcgaggtgat cggcgaggcg gccgacggcg ccgaggcgcg
     1021 cgacctggcg gtgcgcgagc ggcccgacct ggcgatcctc gacgtgaaaa tgccgaaact
     1081 gaccgggctg caggcgaccc gcgagatcaa gaagcaggcg cccgaggtct cggtcctgat
     1141 cctctcgatg cacgacgacg agcgctacct gttcgaggcg ctgaaagcgg gcgcctcggg
     1201 ctacgtgttg aaggcgcagg cggacaccga cctgctggcg gcgatccgcg cggtcgagcg
     1261 cggcgagccg ttcctcaccc cggaggcgca gcggacgctg atcaaggacg tgctcgaacg
     1321 cggctccagc ggcgaggagg agctgacccc gcgcgaggag gagatcgtca agctggtcgc
     1381 cgaggcgcac acgacgaaac agatcgccga gctcctccac ctctccgaga agacggtcga
     1441 gaaccaccgc gccaacgcga tgcgcaagct cgggatgcgc gaccgggtcg agctcgtccg
     1501 gtacgcgatc cggcgcggtc tgatcgagcc ctgacggggc gcaatccaaa cgactgtcgt
     1561 aagtgccgaa cggcataacc gagtgctccg ataggattta gggccagatg ctgttttcga
     1621 ccaaagccga gtacggagtc cgcctgatgg tggagctggg ccgccagccc ggttcgcagc
     1681 cgatctcgct gaacacggtt gccgaggccg agcgcctgcc gctctcctac ctcgagcacc
     1741 tggtggcgaa actgcgcaag gccggcctgg tcacctcgac ccgcggcgcc cacggcggct
     1801 accgcctcgc gctcccggcc gagcagatca cgatggacga ggtagtgcag gcgctggaag
     1861 ggcagatcgc cccgatggag tgcttccacg agacgccgga aggcaaagtc ctctgttcgc
     1921 acgaggacga cggcgaccgc gcctgttcga ccaagctgct ctggacccgg gtgcagggcg
     1981 gcgtcaccaa ggcgctcgcc ggcaccaccc tggccgagct ggtcgagttc tcgcggccgg
     2041 gcggccgcac cgaggcggac atcgccagcg ccgcctaggt ctttcccctc ttcaaaccct
     2101 agaaacggag tcacatggct gagctggaga ttcgaaacct gcacgtcgag gtcgaggaca
     2161 agaagatcct caagggcctc gacctggacg tcgagaaggg caaggttcac gccctgatgg
     2221 gcccgaacgg ctccggcaag tcgacgctcg ccaacgtgat catgggccac ccggcgctcg
     2281 aggtgaccga gggcacgatc atcttcaagg gcgaggacat caccgaggcc tcgcccgagg
     2341 accgctcgca ggccggcctc ttcatggcct tccagtaccc ggtcgcgatc cccggcgtct
     2401 ccgtctccaa gtacctgcgg atgatcatca acgcccagcg cgaggcgcgc ggcgaagacc
     2461 agatcaagat caaagacttc gccaaaaccg ccaaagaggc gatggagctg gcgaacatcc
     2521 cgaacgactt ctccagccgc tacctcaacg acggtttctc cggcggcgag aagaagcgga
     2581 tggagctgct gcagctggcg ctgttgcggc ccgagatcgc ggtcctcgac gagaccgact
     2641 cgggcctcga catcgacgcc ctgcgggttg tcgccgaagg cgtcaacaag ttcgccggcc
     2701 ccgacatggg cgtgctgatc atcacccact accagcgaat cctccacctg gtgaagccgg
     2761 actccgtcca cgtgatgttc gagggccgga tc
//
LOCUS       DASRIG010000134         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2936738, whole genome shotgun sequence.
ACCESSION   DASRIG010000134 DASRIG010000000
VERSION     DASRIG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936738"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFB52_03675"
     CDS             complement(<1..378)
                     /locus_tag="VFB52_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05460.1"
                     /translation="MKSRLHLALLLAALASLLACASAAAAPWIGVRGNHLVNRDGERV
                     RLLGMNRSGTEYTCQQGYGFFDGPSDAASIEVMKSWKINAVRVPLNETCWLGINGVEE
                     ALGGVAYRRAIHAWVSRLEAAGLY"
     gene            419..2329
                     /locus_tag="VFB52_03680"
     CDS             419..2329
                     /locus_tag="VFB52_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HZO05461.1"
                     /translation="MGEKRKDASEQTRDANRRLLDELPFEDERDFEDARRGFIAPLAN
                     GGAVADAAGLPVWDPGRFEFVRESEQAPPTVNPSLWRQSRLVLEGGLFKVTDRLYQVR
                     NQDLSNLTIVEGETGTIVIDPLISTETARAALELYFDHRPRRPVVAVIHSHSHVDHYG
                     GVKGVVSMEDVAAGRVRVIAPAGFLDAAVSENVFAGNAMSRRALYMYGNLLEADPRGQ
                     VGAGLGMTVSRGEISLLEPTEEVTATGQKLEIDGLTFEFLLAPDSEAPAEMHWYIEEL
                     DALTAAENCTHTLHNTYTLRGAPIRDPRAWSRYLNETIDRWGERAEVMYGMHHWPVWG
                     RERVLEMLRKGRDAYRFINDETLRLANHGHTAPEIAELVELPEGLRRHWAVRDYYGTV
                     NHNVKATYVKYLGPYDANPANLHPLPPVEAAARYVEAVGGAARLLELARESFARGEYR
                     WVAELVNHLVFAEPGIAEARELQADALEQMGYQAESGPWRNAYLTAAKELRYGTLDIP
                     LSGTMTPDTVRAMNLGLVCDYLAMRLDGQRAADLEETYELELSDTGERAVLELSNGTL
                     HHSLGRGSEAAEKTTVRLDRATLDRLVLGKTTAAQAVADGALHCEPGPGRFELLLSLL
                     DQFEFWFNLIEP"
     gene            complement(2279..3778)
                     /locus_tag="VFB52_03685"
     CDS             complement(2279..3778)
                     /locus_tag="VFB52_03685"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO05462.1"
                     /translation="MSEHLDVLIVGGGLSGIGAAHHLQRRCPGKTYAILEARERTGGT
                     WDLFRYPGIRSDSDMHTLGYNFKPWTDRKSIADGDLILDYIRDTAREAGIDRNIRLQH
                     KAVRAAWSSEEARWSVEVERGDTGETLTITCDFLYMCSGYYRYDQGYTPEFPGIEDFG
                     GTVVHPQHWPEDLDYAGKRVVVIGSGATAVTLVPAMAKDAAKVTMLQRSPTYVASMPA
                     ADPVAGFLHRFLPARAVYSIVRWKNVLAQLAIYNLSRNRPGILKKFLRRGVERMLPPG
                     YDVDKHFTPRYNPWDQRLCLVPDADLFEAIKDGSAEVVTDRIAGFDQAGIELESGERL
                     DADVVITATGLNVLFLGGMTVSIDGAEPEMARTMTYKGMMLNGFPNFAFALGYTNASW
                     TLKADLVAEYVCRLLNHMDAGGYDVCVPEVTDPTITEEPLLDFNSGYVLRALPGLPKQ
                     GSKEPWKLRQSYPFDLRQMRRGTLLDGSMQFRRLRLDQVEPELELIEQG"
     gene            3856..4515
                     /locus_tag="VFB52_03690"
     CDS             3856..4515
                     /locus_tag="VFB52_03690"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05463.1"
                     /translation="MATHAPAAPEATPRGRRSSRASGDERERAILATAERLLEERPLS
                     EISVGDLAEGAGISRPTFYFYFPSRDAVLLTLVERMADESASAIASQQDALAELHTDP
                     RAGIRQALEQSYAAFSSRRAVILAAAELRNSNADANGLWSRVMEGWVAQVSGIIEAER
                     ARGAAPPGPPAREMATALVQMNERAQHATLSGEQPHIPDDAVIDTLVEIWLRAIYGVA
                     D"
BASE COUNT          696 a         1604 c         1642 g          652 t
ORIGIN      
        1 gtagaggccg gccgcctcga ggcggctgac ccaggcgtgg atcgcgcgcc ggtaggcaac
       61 gccgccgagc gcctcctcga cgccgttgat gcccagccag caggtctcgt tcagcggcac
      121 ccgcacggcg ttgatcttcc agctcttcat cacctcgatc gaggcggcgt cgctgggccc
      181 gtcgaagaag ccgtagccct gctgacaggt gtactcggtg cccgagcggt tcatccccag
      241 cagccgcacc cgctcgccat cgcggttgac caggtggttg ccgcggacgc cgatccaggg
      301 cgccgcggca gcgctggcgc aggccagtag gctggcgagc gcggcgagga ggagcgcgag
      361 gtgcaggcgc gacttcacgc ccgcattgtt gctgggaacc ggaggcaggg gcagggctgt
      421 gggagagaaa cgcaaggacg cgagcgagca gacgcgggac gccaaccggc gcctgctcga
      481 cgagctgccg ttcgaggacg agcgcgactt cgaggacgcg cggcgcggct tcatcgcccc
      541 gctcgcgaac ggcggcgcgg tcgccgacgc cgccggcctg ccggtctggg acccgggccg
      601 gttcgagttc gtgcgggaga gcgagcaggc gccacctacc gtcaacccga gcctctggcg
      661 gcagtcgcgg ctggtgctcg agggcggcct cttcaaggtc accgaccggc tctaccaggt
      721 gcgcaaccag gacctctcga acctgacgat cgtcgagggc gagaccggga cgatcgtgat
      781 cgacccgctg atctcgaccg agaccgcgcg cgccgcgctc gagctctact tcgaccaccg
      841 cccgcgccgc ccggtcgtcg ccgtgatcca ctcgcacagc cacgtcgacc actacggcgg
      901 agtcaagggc gtggtcagca tggaggacgt cgccgccggg cgcgtgcggg tgatcgcccc
      961 ggccgggttc ctcgacgcgg cggtctccga gaacgtcttt gccggcaacg cgatgagccg
     1021 ccgggcgctc tacatgtacg ggaacctgct cgaggccgac ccgcgcggcc aggtcggcgc
     1081 cgggctgggg atgaccgtct cgcgcgggga gatctcgctg ctggagccga ccgaggaggt
     1141 gaccgcgacc ggccagaagc tcgagatcga cggcctcacc ttcgagttcc tgctcgcccc
     1201 cgacagcgag gcgccggccg agatgcactg gtacatcgag gagctcgacg ctctcaccgc
     1261 ggccgagaac tgcacccaca ccctgcacaa cacctacacc ctgcgcggcg ccccgatccg
     1321 cgacccgcgc gcctggtcgc gctacctgaa cgagacgatc gaccgctggg gcgagcgcgc
     1381 cgaggtgatg tacgggatgc accactggcc ggtctggggg cgcgagcggg tgctggagat
     1441 gctgcgcaag ggccgtgacg cctaccggtt catcaacgac gagacgctgc ggctggccaa
     1501 ccacgggcac accgcgcccg agatcgccga gctggtcgag ctgccggagg ggctgcgccg
     1561 ccactgggcc gtgcgcgact actacggcac cgtcaaccac aacgtcaagg cgacctacgt
     1621 caagtacctc ggcccctacg acgccaaccc ggccaacctg cacccgctgc cgccggtcga
     1681 ggcggcggcg cggtacgtcg aggcggtcgg cggcgccgcg cggctgctgg agctggcgcg
     1741 ggagagcttc gcgcgcggcg agtaccgctg ggtcgctgag ctggtcaacc acctcgtctt
     1801 cgccgagccc ggcatcgccg aggcgcgcga gctgcaggcc gacgcgctcg agcagatggg
     1861 ctaccaggcc gagtcgggcc cgtggcggaa cgcctacctg accgcggcca aggagctccg
     1921 ctacggcacc ctcgacatcc cgctcagcgg cacgatgacc ccggacacgg tgcgggcgat
     1981 gaacctcggc ctggtctgcg actacctggc gatgcggctc gacggccagc gcgccgcgga
     2041 cctcgaggag acctacgagc tggagctgag cgacaccggc gagcgggccg tgctggagct
     2101 gtccaatggc accctccacc actcgctcgg ccgcggctcc gaggcggccg agaagaccac
     2161 ggtgcggctc gaccgcgcga ccctcgaccg gctggtgctc ggcaagacca cggcggcgca
     2221 ggcggtcgcc gacggcgctc tgcactgtga gcccggaccg ggccgcttcg agctgctgct
     2281 atccctgctc gatcagttcg agttctggtt caacctgatc gagccgtagg cggcggaact
     2341 gcatcgagcc gtcgagcagc gtgccgcggc gcatctggcg caggtcgaac gggtagctct
     2401 ggcgcagttt ccacggttcc ttcgagccct gtttgggcag gcccggcagc gcccgcagga
     2461 cgtagccgga gttgaagtcg agcagcggct cctcggtgat cgtcgggtcg gtcacctcgg
     2521 gcacgcagac gtcgtagcca ccggcgtcca tgtggttgag caggcggcag acgtattcgg
     2581 cgaccaggtc ggccttcagc gtccaggaag cgttggtgta gccgagcgcg aaggcgaagt
     2641 tggggaagcc gttcagcatc atgcccttgt aggtcatcgt ccgcgccatc tccggctcgg
     2701 cgccgtcgat cgagaccgtc atcccgccga ggaagagcac gttcagcccg gtcgcggtga
     2761 tcaccacgtc ggcgtcgagg cgctcgcccg actcgagctc gatcccggcc tgatcgaagc
     2821 cggcgatgcg gtcggtgacg acctcggcgc tgccgtcttt gatcgcctcg aagaggtcgg
     2881 cgtcgggcac caggcagagg cgctggtccc aggggttgta gcggggggtg aagtgcttgt
     2941 cgacgtcgta gccgggcggc agcatgcgct cgaccccgcg gcgcaggaac tttttgagga
     3001 tcccgggacg gttgcgcgag aggttgtaga tcgccagctg cgcgagcacg ttcttccagc
     3061 gcacgatcga gtagacggcg cgggcgggca ggaagcggtg gaggaagccg gcgaccgggt
     3121 cggcggccgg catcgaggcg acgtaggtcg gcgagcgctg cagcatcgtc accttcgccg
     3181 cgtccttggc catcgccggg acgagcgtga ctgcggtcgc gccgctgccg atcacgacga
     3241 cgcgcttgcc ggcgtagtcg aggtcctcgg gccagtgctg ggggtggacg acggtgccgc
     3301 cgaagtcctc gatccccggg aactcggggg tgtagccctg gtcgtagcgg tagtagccgc
     3361 tgcacatgta taggaagtcg caggtgatcg tcagcgtctc ccccgtgtcg ccgcgctcga
     3421 cctcgacgct ccagcgcgcc tcctcgctcg accaggcggc gcgcacggcc ttgtgctgga
     3481 ggcggatgtt gcggtcgatc ccggcctcgc gggcggtgtc gcggatgtag tcgaggatca
     3541 ggtcgccgtc ggcgatcgac ttgcgatcgg tccagggctt gaagttgtag cccagcgtgt
     3601 gcatgtcgga gtccgaacgg attcccggat agcggaagag gtcccaggtg ccgccggtgc
     3661 gctcgcgcgc ctcgaggatc gcgtaggtct tgcccgggca gcggcgctgc aggtggtggg
     3721 cggcgccgat cccggagagg ccgccgccga cgatcaggac gtcgaggtgc tcgctcatcg
     3781 atcgcagctt atcaacaacg tgtcgagaga tgtcaacggc ttgtcgaatt attcgacgcg
     3841 gtgtatcctg agtcgatggc aacccatgcc ccagccgcac ccgaggcgac tccgcgcggg
     3901 cgccgcagca gccgcgccag cggcgacgag cgcgagcgcg cgatcctcgc gacggcggag
     3961 cgcctgctcg aagagcgccc gctgagcgag atatccgtcg gcgacctggc cgagggcgcc
     4021 ggcatctcgc ggcccacctt ctacttctac ttcccctccc gcgacgcggt cctgctgacc
     4081 ctggtcgaac gcatggccga cgagtcggcg agtgcgatcg cgagccagca ggacgcccta
     4141 gccgagctgc acaccgaccc ccgcgccggc atccgccagg cgctcgagca gtcctacgcc
     4201 gccttctcct cgcgccgcgc ggtgatcctt gccgccgccg agctgcgcaa ctccaacgcc
     4261 gacgcgaacg gcctctggtc gcgggtgatg gagggctggg tcgcccaggt gagcggaatc
     4321 atcgaggccg agcgcgcccg cggcgccgct ccccccgggc cccccgcgcg cgagatggcg
     4381 acggctctgg tgcagatgaa cgagcgggcc cagcacgcga ccctctccgg cgagcaaccc
     4441 cacatccccg acgacgcggt gatcgacacg ctggtcgaga tctggctgcg ggcgatctac
     4501 ggcgtcgccg actgagcgac gccgttgccg ctcggcgcgc cgtcctcgat cacccggaac
     4561 gagatccccg cccgctgcag ccgctccagc agcg
//
LOCUS       DASRIG010000135         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2679597, whole genome shotgun sequence.
ACCESSION   DASRIG010000135 DASRIG010000000
VERSION     DASRIG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679597"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFB52_03695"
     CDS             complement(<1..655)
                     /locus_tag="VFB52_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05464.1"
                     /translation="MAADPKTPDSATEYPPELSRLPPGRHGLPREFVVHNQRERLIAG
                     LAEAVAENGYASTTIAHITRHAAVSRRTFYEHFNSKDECFVAAYEAVMEQLRERVDAA
                     YQEQDEWPQALRAGLAAMLSFLAAEPNLARLSMVEALVAGPVVVERYDAAIRGLVPYV
                     EAGRDGRPSAVLAQLSPTTEEALVGGMVSLISRRIIAGQTEELEQLLPDLVEFKLTPY
                     "
     gene            complement(707..1336)
                     /locus_tag="VFB52_03700"
     CDS             complement(707..1336)
                     /locus_tag="VFB52_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05465.1"
                     /translation="MRPPPGRQQLSREFITQHQRARIIAALAEETVTRGYRAVTVADI
                     VRRAGIARNTFYENFSSKQDCFLATQDYAVEEALRRVVDAATEVESWQERVHAGLAAF
                     LGYVASDPALARTCIVEALSAGPASLARYEESLQSFVPLFRIGRKVSPRAEQLPETFE
                     ELIIGGIFWVIYQRIIRGETEQIEQLLPELVEFALNPYIGAEAARRATA"
     gene            1514..2191
                     /locus_tag="VFB52_03705"
     CDS             1514..2191
                     /locus_tag="VFB52_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05466.1"
                     /translation="MDSSAVPGPPKGGPANEEMRQRVYAVRSDRERLLEAMVRVSAER
                     GYEATAVADVVARADLEEGAFERHFNDKEDCFLAAYQATSDVLVALTSTAFEGAGERP
                     WAERVAVALRAMVELLAAESEVARMAVVEVTAIPGDARVRSRELLDRFIPFLEQGREV
                     SEQGAELPAETARFAIGGASSLIFDELRAGRGPELEAILPDLVFAVTMPYLGVAAAEA
                     EMRKISI"
     gene            complement(2222..2974)
                     /locus_tag="VFB52_03710"
     CDS             complement(2222..2974)
                     /locus_tag="VFB52_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO05467.1"
                     /translation="MAEEVRYERQGAAAVLTIDRPERRNAVDGATARALRAGFERFEA
                     DDEARVLILTGAGGEAFCAGADLKALDLDVDDPAGPMGFSRLTPSKPAIAAVDGWCLA
                     GGLELALWCDLRLATPASTFGYFERRWGVPLVDGGTQRLPRVIGLGRALDLILTGRPV
                     EAEEAQRIGLVNELVAPGAQLERALELAERIASFPQETMLADRRAAIEGLGMPLGEGL
                     ALEHRLGRETLEVAVRGAARFAGGEGRHGSGV"
     gene            3484..4872
                     /locus_tag="VFB52_03715"
     CDS             3484..4872
                     /locus_tag="VFB52_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HZO05468.1"
                     /translation="MNEGTAASARRARRNVVLTAAIAGLGGLLFGYDTGVIAGALLFI
                     KPEFDLGSFAQGLVVAAVPIGAVAGAAIAGPAADKYGRRVMILISAGVFIVGALGSAA
                     APGVEVLVIARIVIGVAIGVASAAAPVYISEVAPPESRGQLVTFFQLAVTVGILVAYL
                     VGLAFDGIEGWRWMLGLGCVPALALAFGMIRMPQSPRWLVMTGDDYAARATLAKIRVD
                     DPDTIDREIEEIHEGLDEKPGAWSELLQPVVKAALVVGVGLAILQQVTGINTVIYYAP
                     TIVEFTGVDSSAGSILAAVGVGVINVLATIVALRLLDKAGRRTMLMIGVTGMTVSLFT
                     LGLAFTGNDSSTLTSVVAIGSLMAFVASFAISLGPIFWLLNAEIYPLSVRSKAAGIGT
                     MANWTFNFIVSLTFLLLIEALGRSGAFWFYAAVGGLTLIFCWKLVPETKGKHLEDIQA
                     EFQARVDRRHKS"
     gene            4891..>5336
                     /locus_tag="VFB52_03720"
     CDS             4891..>5336
                     /locus_tag="VFB52_03720"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO05469.1"
                     /translation="MRIDEILAAKRPIFSVEFFPPKTPEATEQLYATARELEPLEPDF
                     VSVTYGAGGSTRDGTVEITAALKDEVGLETMAHLSCVGETTEGLAATLDRIEAAGVEN
                     VFALRGDPPRGERDFVQPEGGLGSAAELAAYVSEGWDFSIGGACFP"
BASE COUNT          787 a         1798 c         1937 g          814 t
ORIGIN      
        1 ggtaaggcgt cagtttgaac tcaaccaggt cggggaggag ctgctccagc tcctccgtct
       61 gcccggcgat gatgcggcgg gagatcagcg agaccatgcc gccgaccagc gcttcctcgg
      121 tggtcggcga gagctgcgcg agcaccgcgg acgggcggcc gtcgcgaccc gcctcgacgt
      181 aggggacgag gccgcggatc gcggcgtcgt agcgctcgac cacgaccggg ccggcgacca
      241 gggcctcgac catgctgagg cgagcgaggt tgggctcggc ggcgaggaac gagagcatcg
      301 cggcgagccc ggcgcgcagc gcttgcggcc actcgtcctg ttcctggtag gccgcgtcga
      361 cccgctcgcg gagctgctcc atcaccgctt cataggcggc gacgaagcac tcgtccttgc
      421 tgttgaagtg ctcgtagaag gtgcgccggg agaccgccgc gtggcgggtg atgtgggcga
      481 tcgtggtgct ggcgtagcca ttttcggcga ctgcctcggc gagcccggcg atcagtcgct
      541 cgcgctggtt gtggacgacg aactcgcgcg gcaggccgtg ccgtccaggg ggtagccgcg
      601 agagctcggg cgggtactca gtggccgagt cgggggtctt tggatcagcg gccacgaaag
      661 aaaacgatac tggtagggct gttttccaat acgccgttcg gggggctcag gcggtcgccc
      721 gccgggccgc ttcggcgccg atgtaagggt tcagggcgaa ctcgaccagc tccggcagca
      781 gctgctcgat ctgctcggtc tcgccgcgga tgatccgctg gtagatcacc cagaaaatgc
      841 cgccgatgat cagctcctcg aaggtctccg gcagctgctc ggcgcgcggg gagaccttgc
      901 ggccgatccg gaagagcggg acgaaggact gcagcgactc ctcgtagcgg gccagcgaag
      961 cgggcccggc ggagagcgcc tcgacgatgc aggtgcgggc cagtgccggg tcgctggcga
     1021 cgtagccgag gaaggcggcg aggcccgcgt ggacgcgctc ctgccagctc tcgacctcgg
     1081 tggccgcgtc gaccacccgc cgcagcgcct cctcgaccgc gtagtcctgg gtcgctagaa
     1141 agcagtcctg cttggaggag aagttctcgt agaaagtgtt gcgggcgatc ccggcgcggc
     1201 gcacgatgtc ggcgacggtg accgcgcggt aaccgcgggt gacggtctcc tcggcgaggg
     1261 cggcgatgat ccgcgcgcgc tggtgctggg tgatgaactc gcgggagagc tgctggcgcc
     1321 ccgggggcgg tcgcatggtt gagcttcgct gcgtcacgaa cgccagggta cctgagccgg
     1381 actcgattca gtccggaaaa aaggttgtca agcatgccgc atactcgtcg agttcttcat
     1441 gaagatcaac cccactccac caatggagtt ggttccttcg gtcgatcact acagcctgag
     1501 taggctcgta cgcgtggatt cgtcagcagt accgggtccg ccgaaagggg gaccggcaaa
     1561 cgaggagatg cggcagcgcg tctacgcggt gcgcagcgac cgcgagcgcc tgctcgaggc
     1621 gatggtccgg gtcagcgccg agcgcggcta cgaggccacg gccgtggccg acgtggtcgc
     1681 gcgcgcggac ctcgaggaag gcgccttcga gcgccacttc aacgacaagg aggactgctt
     1741 tctcgctgcc taccaggcga cgagcgatgt cctcgtagcc ctgaccagca ctgcctttga
     1801 gggcgctggg gagcgaccct gggcggagcg ggtggcggtc gcgctgcggg cgatggtcga
     1861 gctgctcgcg gccgagtccg aggtcgcgcg catggcagtg gtcgaggtga cggcgatccc
     1921 cggcgacgcc cgggttcgca gccgcgagct gctcgaccgc ttcatcccct tcctcgagca
     1981 gggacgtgag gtcagcgagc agggagcgga gctgccggcg gagaccgctc gcttcgcgat
     2041 cggcggcgcc tcctcgctga tcttcgacga gctgcgggcc ggtcgcggtc ccgagctgga
     2101 ggcgatcctg cccgacctgg tgttcgcagt gacgatgccc tacctgggcg ttgctgccgc
     2161 ggaagcggag atgcgcaaga tcagtatttg aactgacttt ggttcaacag agtttgggcg
     2221 cctagacgcc gctgccgtgg cggccctcgc cgccggcaaa gcgggcggcg ccgcggaccg
     2281 cgacctcgag cgtctcccgg cccaaccggt gctcgagcgc gagcccctcg ccgagcggca
     2341 ttcccagccc ctcgatcgcg gcgcggcgat cggcgagcat cgtctcctgg gggaaggagg
     2401 cgatccgctc ggccagctcc agcgctcgct ccagttgggc gcccggggcg accaactcgt
     2461 tgaccagacc gatccgctgc gcctcctcgg cctcgaccgg gcggccggtg aggatcaggt
     2521 cgagggcgcg gccgagaccg atgacgcggg gcagccgctg ggtgccgccg tcgacgaggg
     2581 gcacgcccca gcgacgctcg aagtagccga aggtggaggc gggagtggcg agccgcaggt
     2641 cgcaccagag cgccagctcg agcccgccgg ccaggcacca gccgtcgacc gcggcgatcg
     2701 ccggcttcga gggcgtgagg cggctgaagc ccatcggccc ggcggggtcg tcgacgtcga
     2761 gatccagggc cttgaggtcg gcgccggcgc agaaggcctc gccaccggcc ccggtcagga
     2821 tcagcacccg tgcctcgtcg tcggcctcga agcgctcgaa gccagcgcgc agagcgcgcg
     2881 cggtcgcgcc gtcgaccgcg ttgcgacgct ccgggcggtc gatcgtcagc accgccgccg
     2941 ccccctgtcg ctcgtagcga acctcctccg ccatacccgc gccatcctcc cacaggggcg
     3001 ccaaccattt cgaaccactg atggaggtgg gagcgggcgg gctagtcgca ggcgctggct
     3061 cacggggttc gccggcgacg cccaactccc ggcgcggtgc gccccggtcg cgggctggga
     3121 gggcaggtct cccgagccgc cggcaacgct aggcgccagg tcggacgaaa gagccgtgaa
     3181 gcgagtcccc cacccggcac ccgccccacg gccacgaaaa agcgaccgag gggggcgctc
     3241 gtcgcctgcc ttgcgcgtag ccttcgcgct cagaagctcg gttgcgccac cttcgaaacc
     3301 gcttcccgct tggcttccgc ggggcccggt ctctctcggt cgcagggcga acttacgtgg
     3361 ccgcgcgaaa gcgcgcaagg ggtgtgcgcg ggacgattcc gcaaatacgg gggtaatggg
     3421 tatttgctcg gcgccaaacg gggggtggcg cggcgccgag cgccggtata cggttgccgc
     3481 gggatgaacg agggaaccgc cgcctccgct cgcagggcgc gccgcaacgt ggtcttgacg
     3541 gccgcgattg ccggactcgg cggcctcctg ttcggctacg acaccggcgt catcgccggg
     3601 gcgctgctgt tcatcaagcc cgagttcgac ctcggcagct ttgcgcaggg cttggtcgtc
     3661 gccgcggtcc cgatcggcgc cgtcgccggc gccgcgatcg ccgggccggc cgccgacaag
     3721 tacggccgcc gcgtgatgat cctgatctcc gccggcgtgt tcatcgtcgg cgcgctcggc
     3781 agcgccgcgg cgccgggggt cgaggtgctg gtgatcgccc gcatcgtgat cggcgtcgcg
     3841 atcggcgtcg cctcggcggc ggcgcccgtc tacatctccg aggtcgcgcc gccggagagc
     3901 cgcgggcagt tggtcacctt cttccaactc gcggtcaccg tcggcatctt ggtcgcctac
     3961 ctggtcgggc tcgccttcga cggaatcgaa ggctggcgct ggatgctcgg gctcggctgt
     4021 gttccagcgc tggcgctcgc cttcggaatg atccggatgc cgcagagccc gcgctggctg
     4081 gtgatgaccg gcgacgacta cgccgcccgg gcgacgctgg cgaagatccg ggtcgacgac
     4141 ccggacacga tcgaccgcga gatcgaagaa atccacgaag ggctggacga gaagcccgga
     4201 gcctggagcg agctgctgca gccggtggtg aaagcggcgc tggtggtcgg cgtcgggctg
     4261 gcgatcctgc agcaggtgac cgggatcaac acggtcatct actacgcgcc gacgatcgtc
     4321 gaattcaccg gcgtcgactc ctccgccggc tcgatcctgg ccgcggtcgg ggtcggcgtg
     4381 atcaacgtcc tggcgacgat cgtcgccctg cgcctgctcg acaaagccgg gcgccgcacg
     4441 atgctgatga tcggcgtcac cgggatgacg gtctcgctgt tcacgctcgg gctcgccttc
     4501 accggcaacg acagctcgac gctcacctcg gtcgtcgcga tcggcagcct gatggccttc
     4561 gtcgcctcct tcgcgatcag cctcgggccg atcttctggc tgctcaacgc cgagatctac
     4621 ccgctcagcg tgcgcagcaa agcggccggg atcgggacga tggcgaactg gaccttcaac
     4681 ttcatcgtct cgctcacctt cctgctgctg atcgaagcgc tcggccgctc cggagccttc
     4741 tggttctacg ccgcggtcgg cggcctcacc ctgatcttct gctggaagct ggtgccggag
     4801 accaagggca agcacctcga ggacatccag gccgagtttc aggcgcgcgt cgatcgccgc
     4861 cacaagtcgt agcgtcgcta ctctccccgg atgcgcatcg acgagatcct cgccgccaaa
     4921 cggccgatct tctcggtcga gttcttcccg ccgaagacgc cggaggcgac cgagcagctc
     4981 tacgcgaccg cgcgggagct ggagccgctg gagccggact tcgtctcggt cacctatggg
     5041 gccggtggct cgacccgcga cggcacggtg gagatcaccg cggcgctcaa ggacgaggtc
     5101 gggctggaga cgatggccca cctgagctgc gtcggcgaga cgaccgaggg gctggcggcg
     5161 acgctcgacc ggatcgaggc ggccggggtc gagaacgtct tcgccctgcg tggcgacccg
     5221 ccgcgcggcg aacgcgactt cgtccagccc gagggcgggc tgggcagcgc cgccgagcta
     5281 gccgcatacg tttcggaggg ctgggacttc tcgatcggcg gggcctgctt tccaga
//
LOCUS       DASRIG010000136         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1123265, whole genome shotgun sequence.
ACCESSION   DASRIG010000136 DASRIG010000000
VERSION     DASRIG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123265"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1009
                     /locus_tag="VFB52_03725"
     CDS             164..1009
                     /locus_tag="VFB52_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05470.1"
                     /translation="MTSEPSRQDNGAQDNGIPRGALIIVAIGVLACLAAAVLATDKSS
                     GAAANLEWVKTAKIPDSKVVEVPGGGGEMRLEKAQIAATGTNVSGYSLFRVATTLRID
                     GGSPVGDAQILCETKGGPRAEIARSSGGLRALYPRSADNGIYSQSVPEVLLLDFSARG
                     GELVVVEAEDLLIPRFTSEKGVKLEWPEYEEGVERLKYFVAGGKPENDLVLPFYSVWK
                     STAVPSAEISCTVTTSAGKSTASTAGGLKKMPPPIDEEAEDEAEEQAEEEADELEEDE
                     AEGNE"
     gene            complement(1016..1810)
                     /locus_tag="VFB52_03730"
     CDS             complement(1016..1810)
                     /locus_tag="VFB52_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZO05471.1"
                     /translation="MCGRFTVTKKDTKSITDRFQVELEKALERGEENKDGGKEKAPPG
                     LGRYNVAPTQEILVVRSSPEPEEAAAGEREARLMRWGLVPVWAKDLKVGYRMINAKTE
                     NLESSRAYSPLVGKYRHRCLIVADGFYEWMKAEDPKQPRQPWRFTVDGGELFAFAGLC
                     TRKEWADPDDRGVADDGYLYSATIITTTPNKVVAPVHNRMPSILPSREAEAAWLNPEL
                     STEDAIAMLGPLDAARMEGAPANPKLNKVGKGMAEGPDLLVAPDDA"
     gene            complement(1842..2252)
                     /locus_tag="VFB52_03735"
     CDS             complement(1842..2252)
                     /locus_tag="VFB52_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZO05472.1"
                     /translation="MTTATLVDSNVLIDVFGGDGPWTSRSATALEAAANAGPLVINQV
                     IYAEIAVRYEDTDELDGDLPRDVFQRESVPWSAAFLAGKAFEIYRGRGGVRRSPLPDF
                     FIGAHAAVTGLRLLTRDASRYRSYFPTVELIAPE"
     gene            complement(2230..2517)
                     /locus_tag="VFB52_03740"
     CDS             complement(2230..2517)
                     /locus_tag="VFB52_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZO05473.1"
                     /translation="MRITSKGQVTIPKAIRDQFGLLPGTEVEFVEKGGEIQVRKVEGG
                     ERSRGREIVEHMQRPGAGGTNRLTTEEIMRMTRGEDWGTGRLAEDDHGDAR"
     gene            complement(2557..>3025)
                     /locus_tag="VFB52_03745"
     CDS             complement(2557..>3025)
                     /locus_tag="VFB52_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO05474.1"
                     /translation="VAVTRFANIYGGGDANFSRLIPEAIAAVLDGRRPQIRSDGSPER
                     DFLYVADAVAAYFAVESAVGAGGPGAGEAFNAGGEQPHTVREVLETISEVSGSGLDPE
                     YLGTGNPAGEIDRQYVDSTKLRELTDWEPAVGLRDGLIRTLDWYRDHPELRPG"
BASE COUNT          505 a         1039 c          964 g          517 t
ORIGIN      
        1 ggtgcagctc tgcgctttgg ctgagtcgat cgggaccacc ttgcgcctca tacccccacg
       61 ttagctacag ggaacgctgt aaccgtcggg tgagtcggct ctgtgacgga tcgcaagcag
      121 cgccgagcga gcgtctgact gattccaata acgtcgcaac ggcatgacga gcgagccgtc
      181 gcgacaggac aacggagcgc aggacaacgg gatcccgcgc ggggcgctga tcatcgttgc
      241 gatcggcgtt ctcgcctgcc tcgccgctgc ggtgctggcg accgacaaga gctccggcgc
      301 ggcggccaac ctcgaatggg tcaagaccgc gaagatcccc gattccaagg tggtcgaggt
      361 gccgggcggc ggtggtgaga tgcggctcga aaaagcgcag atcgccgcga ccgggaccaa
      421 tgtcagcggc tactcgctgt ttcgcgtcgc caccaccctg cgcatcgatg gcggctcgcc
      481 ggtcggcgac gcccagatcc tctgcgagac caaaggcggc ccccgggccg aaatcgcccg
      541 ctcctccggt ggcctccggg cgctctaccc gcgctcggcc gacaacggga tctacagcca
      601 gagcgtgccg gaagtgctgc tgctcgactt cagcgcccgc ggtggcgaac tcgtcgtggt
      661 cgaagccgaa gacctcctga tcccccgctt cacgagcgaa aaaggcgtca agctcgaatg
      721 gcccgaatac gaagaaggcg tcgagcgcct caaatacttc gtcgccggcg gcaaaccgga
      781 aaacgatctc gtgctgccct tctactcggt ctggaagtcg accgccgtgc cctccgccga
      841 gatctcctgc acggtcacga ccagcgccgg caaatcgacc gcgagcaccg caggcggcct
      901 gaagaagatg ccgccgccga tcgacgaaga agccgaagac gaagccgaag aacaggccga
      961 agaagaagcc gacgaactcg aagaggacga agccgaaggc aacgagtagc ggcactcagg
     1021 cgtcgtcggg ggcgaccagc agatccggcc cctccgccat tcctttgccg actttgttca
     1081 gcttcgggtt cgccggcgcc ccttccatgc gggccgcgtc gagcgggccc agcatcgcga
     1141 tcgcgtcctc ggtgctcagc tccggattga gccaggccgc ctcggcctcc cggctgggca
     1201 ggatcgatgg catccggttg tggaccggcg ccaccacttt gttcggcgtc gtggtgatga
     1261 tcgtggcgct gtagaggtag ccgtcgtcgg cgaccccgcg atcgtcgggg tccgcccact
     1321 ccttgcgcgt acagagcccg gcgaaggcga agagctcacc gccgtcgacg gtgaagcgcc
     1381 agggctggcg cggctgcttg ggatcctcgg ctttcatcca ctcgtagaag ccgtcggcca
     1441 cgatcaggca gcggtggcgg tacttgccga ccagcggcga gtaggcgcgg ctcgattcga
     1501 ggttctcggt tttcgcgttg atcatccggt agccgacttt gaggtccttg gcccagaccg
     1561 gcaccaggcc ccagcgcatc agccgcgcct cgcgctcacc ggcggccgcc tcctcgggct
     1621 cgggcgagga gcgcacgacg aggatctcct gggtcggcgc gacgttgtaa cggccgagcc
     1681 cgggtggcgc tttctccttg cccccgtcct tgttttcctc gccccgttcg agcgctttct
     1741 cgagctcgac ctggaagcga tcggtgatcg acttcgtgtc cttcttggtg accgtgaagc
     1801 gcccgcacat ggggctcaga ctagtaactc gccccggcgc gctactcggg cgcgatcagc
     1861 tccacggttg ggaagtagct tcgatagcgc gaggcatcac gagtgaggag gcgcaggccc
     1921 gtcaccgcag cgtgcgcgcc gataaagaag tcgggcagag gcgatcgacg gactccgccc
     1981 cgccctcggt agatctcgaa cgcctttccg gcaaggaatg cggctgacca cggcaccgac
     2041 tcacgttgaa aaacgtcgcg gggaagatcc ccgtccagtt cgtcggtgtc ctcgtagcgc
     2101 accgcaatct cggcgtagat cacctggttg atcaccagcg gccctgcatt ggccgccgct
     2161 tccagcgccg ttgccgacct actcgtccat ggcccatccc caccgaagac gtcgatcaag
     2221 acattggaat caacgagcgt cgccgtggtc atcctccgct aagcgaccgg tgccccaatc
     2281 ctccccccgg gtcatccgca tgatctcctc ggtagtcagg cggttggtcc ctcctgcccc
     2341 gggtctttgc atgtgttcga cgatttcacg tcctcgactt ctctctcccc cctcgacctt
     2401 gcgcacctga atctcgcccc ctttctcgac gaactccact tccgtcccgg gcagcaatcc
     2461 gaactggtcg cggatcgcct tcgggatcgt cacctggcct ttgctcgtga tcctcatgaa
     2521 agtaagagta gcaccggtat tactcttact ttacgctcaa ccaggccgta gctctgggtg
     2581 gtcccggtac cagtcgagcg tgcggatcag gccgtcgcgc aggcccaccg ctggctccca
     2641 atcggtcagc tcgcgcagct tggtcgagtc gacgtactgg cggtcaatct caccagcggg
     2701 gttgccggtg ccgaggtact cggggtcgag gcccgacccc gagacctcgg agatcgtctc
     2761 cagcacctcg cgcaccgtgt gcggctgctc gccgccggcg ttgaaggcct cccccgcccc
     2821 gggcccgcca gcgccgaccg cgctctcgac cgcgaagtag gcggcaaccg cgtcggcgac
     2881 gtagaggaag tcgcgttcgg ggctgccgtc ggagcggatc tgcgggcgcc ggccgtcgag
     2941 cacggcggcg atcgcttccg ggatcaggcg cgagaagttg gcgtcgccgc cgccgtagat
     3001 attggcgaag cgggtgaccg cgacc
//
LOCUS       DASRIG010000137         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2937403, whole genome shotgun sequence.
ACCESSION   DASRIG010000137 DASRIG010000000
VERSION     DASRIG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937403"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFB52_03750"
     CDS             complement(<1..1310)
                     /locus_tag="VFB52_03750"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HZO05475.1"
                     /translation="MEAAPEYDSPIDDPALYFNRELSWLEFNQRVLELAEDESVPLLE
                     RLRFCGIYASNLDEFFMVRVAGLFDQLDAGIDARGPDGLAPSEQIDRIQARVLELDTR
                     LHACFSGILRPALEQHGIRILTLETASEEERREIDASFHEQVFPALTPLVIGLGRPFP
                     YISNLSLSLGVLLRDPESGAEIIARVKVPKELMGRFIQVGEGGKSFVPLEEVIAANLD
                     ALFPGTEVVDHGYFRVTRDADFTVSDEADDLLQAVQDEIRRRRFGEVVRLEIAAGMNP
                     KLREQLIEALRLEDREVYDVDGLIDLGDVDAIADVPGHAELRYSPWSPVTQPRLQGED
                     EEPVDMFASIRRGDVLVHHPYDSFANSVERFVAQAVADPDVLAIKQTVYRTSDDSPLV
                     PWLIRASERGKQAVCMVELKARFDEAANIHWAKALEEAGVHVVYG"
     gene            complement(1321..>2721)
                     /locus_tag="VFB52_03755"
     CDS             complement(1321..>2721)
                     /locus_tag="VFB52_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO05476.1"
                     /translation="VGLELRGRELIDHLQADNFSHSDLYRRARRTHDRRLRVAVEQGS
                     AAFTVGDFPSAMNGVFEALLPALPYAPATAFLGAEVNRLRGRSDEPRRVAVIADGIGS
                     MHGVTHTIEQIRERGVPGFEVDVVGTDPGVDRRLPAATELEIPFYEGLPLGVPGLPDL
                     VETLAEGNYELIHVTAPGPAGIAATMLSRVTGIPLLASYHTELAAYAGMRSDDGGIEA
                     IARGALGAFYRVPGVVLSPSPAADASLAGLGLDPARVRRWERGVDLDRFDPAKARRDE
                     YPGEIKVLYVGRLTREKGVDLLAESFLRAHRSDPRLHLLLAGGGPEEAELRARLGEHA
                     TFLGWLGGEDLAEAYASADVFLFASSTDTYGQVIIEAAASGLPVVAVAEGGPAALVEN
                     RHTGLLCRPDADHLAGALLQLASSPLLRHHLGTSGAEAARGRSWERSMDQLAAGYRRL
                     LESEAAGAEQAFVQAA"
BASE COUNT          388 a          958 c         1006 g          369 t
ORIGIN      
        1 ccgtagacga cgtggacccc cgcctcctcc agcgctttcg cccagtggat gttcgccgcc
       61 tcgtcgaagc gggctttcag ctcgaccatg cagaccgcct gcttgccgcg ttcggaggcg
      121 cggatcagcc acggcaccag cggcgaatcg tcgctggtcc ggtacaccgt ctgcttgatc
      181 gccagcacgt cggggtcggc caccgcctgg gcgacgaagc gctcgaccga gttggcgaac
      241 gagtcgtagg gatggtggac gaggacgtcg ccgcggcgga tcgacgcgaa catgtcgacc
      301 ggctcctcgt cctccccctg caggcgcggc tgggtgaccg gcgaccaggg cgagtagcgc
      361 agctcggcgt ggcccgggac atcggcgatc gcgtcgacgt cgccgaggtc gatcagcccg
      421 tcgacgtcgt agacctcacg gtcttcgagc cgcagcgcct cgatcagctg ctcgcgcagc
      481 ttcgggttca tcccggcggc gatctccagc cgcaccacct cgccgaagcg gcggcggcgg
      541 atctcgtcct ggaccgcctg caggaggtcg tcggcctcgt cggagacggt gaagtcggcg
      601 tcgcgggtga cccggaagta gccgtgatcg acgacttcgg tgcccgggaa cagcgcatcg
      661 agattggccg cgatcacctc ctccaacggc acgaacgact tgccgccctc gccgacctgg
      721 atgaaacggc ccatcagctc cttcggcacc ttgacccggg cgatgatctc ggccccgctc
      781 tcggggtcgc gcagcagcac gccgaggctg agcgagagat tggagatgta gggaaagggc
      841 cggccgaggc cgatcaccag cggcgtcagc gccgggaaga cctgctcatg gaagctagcg
      901 tcgatctcgc ggcgctcctc ctcgctggcg gtctcgagcg tgaggatgcg gatcccgtgc
      961 tgctcgaggg ctggccgcag gatgccggag aagcaggcgt gcaggcgcgt gtccagctcc
     1021 agcacccgcg cctggatgcg gtcgatctgc tcgctcggcg ccagcccgtc ggggccgcgc
     1081 gcgtcgatcc cggcatcgag ctggtcgaac agaccggcga cccggaccat gaagaactcg
     1141 tcgaggttgc tggcgtagat cccgcagaag cgcagtcgct ccagcagcgg cactgactcg
     1201 tcctcggcca gctccagaac ccgctggttg aactcgagcc aggaaagctc gcgattgaaa
     1261 taaagagcgg gatcgtcgat cggcgaatcg tactccggcg cggcttccac ccttgccctc
     1321 tcaggcggcc tggacgaatg cctgctcggc gccggccgcc tcgctctcca gcaggcgccg
     1381 gtaaccggcg gcgagctggt ccatcgagcg ctcccaggag cggccgcggg ccgcttcggc
     1441 gccgctcgtc cccaggtggt ggcgcagcag cggcgaggag gcgagctgca gcagggcgcc
     1501 ggccaggtgg tcggcgtcgg ggcggcagag cagcccggtg tggcggttct cgaccagcgc
     1561 cgccggcccg ccctcggcga ccgcgacgac cggcagcccg ctggccgccg cctcgatgat
     1621 cacctggccg taggtgtcgg tggagctggc gaagaggaaa acgtcggcgc tggcgtaggc
     1681 ctcggcgagg tcctcgccgc cgagccagcc gaggaaagtc gcgtgctcgc cgagccgggc
     1741 gcgcagctcc gcctcctcgg ggccgccacc ggccagcagc aggtgcaggc gcgggtcgga
     1801 gcggtgggcg cggaggaagc tctcggcgag caggtcgacg cccttctcgc gggtcaggcg
     1861 gccgacgtag aggaccttga tctcgcccgg gtactcgtcg cggcgggcct tggccgggtc
     1921 gaagcggtcg aggtccaccc cgcgctccca gcgccgcacc cgggccgggt cgagcccgag
     1981 gccggcaagc gaggcgtcgg cggccgggct cggcgagagc acgaccccgg gcacccggta
     2041 gaaggcgccg agggcgccgc gcgcgatcgc ctcgatcccg ccgtcgtcgc tgcgcatccc
     2101 cgcgtaggcg gccagctcgg tgtggtagct ggcgagcagc gggatgccgg tgacccggct
     2161 gagcatcgtc gcggcgatcc cggccggccc cggcgcggtc acgtggatca gctcgtagtt
     2221 gccctcggcg agcgtctcga ccaggtcggg caggcccggg accccgagcg gcagcccctc
     2281 gtagaagggg atctcgagct cggtcgcggc cggcaggcgg cggtcgacgc ccgggtcggt
     2341 gccgaccacg tcgacctcga acccgggcac gccgcgctcg cggatctgct cgatcgtgtg
     2401 cgtgaccccg tgcatcgagc cgataccgtc ggcgatcacg gcgacccggc gcggctcgtc
     2461 ggagcggccc cgcaggcggt tcacctcggc gccgaggaag gcggtcgcgg gcgcgtaggg
     2521 cagggccggc agcagcgcct cgaagacccc gttcatcgcg ctcgggaagt cgcccacggt
     2581 gaacgccgcg ctcccctgct cgaccgcgac gcgcaagcgc cggtcgtggg tgcggcgggc
     2641 acggcggtag agatcggagt gggagaagtt gtcggcctgc aggtggtcga tcagctcgcg
     2701 gccgcgcagc tccaggccga c
//
LOCUS       DASRIG010000138         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3722302, whole genome shotgun sequence.
ACCESSION   DASRIG010000138 DASRIG010000000
VERSION     DASRIG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722302"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFB52_03760"
     CDS             complement(<1..223)
                     /locus_tag="VFB52_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO05477.1"
                     /translation="MSTGRILIADDEPSVRDSVGYALTQEGFEVTAAADGDEATELLG
                     NGIPYDLLILDIMMPGPSGLDICRDVRSRS"
     gene            complement(246..1604)
                     /locus_tag="VFB52_03765"
     CDS             complement(246..1604)
                     /locus_tag="VFB52_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO05478.1"
                     /translation="MSPPNETPAPPRPGAPRIGAPRIGMRLWLTGAFGSVSLITAAAV
                     YLFGDNVQALVAAVMIGVLAGFLIAVAISHRVVRLARAAKEMAGGSFDAPLNVSGPDE
                     IGDLARALDSMRASLKESFGVLSSDRNKLAAIFDGLTDAVIVVDFEGPIRFSNKAASR
                     LLDSAGRPPDVVLPQLHRASEVGFAAHPALRIGDHAYAMTARSLPDERAVLAVLRDRT
                     DELRRELAEADFVSNAAHELRNPLAGISGAIEVLQGGAKNDPVDRDHFLNRLADDAER
                     MNRLTQSLLTLARVESRGAAESEVVDVPAAIRDVVAAVEEPKHVALEVDCESDLAATG
                     DPTLLRQVLIGLVTNAFKNTPPPGTVTVRGRHDGEGEVIVEVIDTGTGIPAAEIERVF
                     ERFYRRSESRKQEGFGLGLAIARRMVDVMGGQIGADSTLGEGSTFWVRLPVAQPSPTP
                     VA"
     gene            1686..2414
                     /locus_tag="VFB52_03770"
     CDS             1686..2414
                     /locus_tag="VFB52_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO05479.1"
                     /translation="MRTLVFIPAWNEQASISAVVADVREHLPKADILVVDDGSTDATT
                     RTAREAGVLVATLPFNQGLGAALQTGYLLALREGYDCCAHLDADGQHPAEEVARLLAE
                     VEGDRADLVIGSRYTDPDAEAGVDDYRPTFSRRIGTSVFRFFLTLATRQRFTDTTSGM
                     RAANRKVMALFSEHYSPDFAEIESLQLAVREGLRVQEVPVRMLERVGGSSFLTPLKSA
                     FFIFKGVVVLLVGQFRPRRVEAER"
     gene            2411..2869
                     /locus_tag="VFB52_03775"
     CDS             2411..2869
                     /locus_tag="VFB52_03775"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HZO05480.1"
                     /translation="MSLPLAASDPNTVHLTAQTRILAAILAIIFMFAILELIRRDKLQ
                     ERYSVIWFVAGLGMLAGAAFPGLLELVADAMGVRDTNVALFSIVLFFLLGLALNFSVI
                     MSRQAAQITRLAQERALEGVRDQEAGRRNGNGAEPAVVDPPAVKPPGESP"
     gene            2866..3444
                     /locus_tag="VFB52_03780"
     CDS             2866..3444
                     /locus_tag="VFB52_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05481.1"
                     /translation="MTYLRSKLTYANVVATLALCIAVGGASAFAANQLAKNSVGTKQL
                     KKNAVTTAKIKNRAVTGAKLKTATIGTVPSAQVANTLAAPEPWHEVGKPGEPSFLNGW
                     DHYPGSPLVAFYRDAGGVVHLRGAVTGANEETAAFFLPAGFRPAATQSFASWGGPQAL
                     IVIPASGEVRPYCGTGGVCATGLEGISFRAEG"
     gene            complement(3464..>4553)
                     /locus_tag="VFB52_03785"
     CDS             complement(3464..>4553)
                     /locus_tag="VFB52_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05482.1"
                     /translation="LLEATEHQGRARIALTAALESGPSHAYLFRGPRGAGKRAAARAL
                     AAEILADGAADPEDARRRALLDPSPHPDLVWLVPRGAQHLVDEVRERVIRAAAYRPFE
                     GDKRVFVVEAAEAMRDESQNALLKTLEEPPAYVHLILLSSEPEALLETIASRCQPVDF
                     APLPAQVLEAALAGSGAEAEVAAAARLAGGDLERARLLLSPRGRELRGEAERCLAAAL
                     ADEVGSAPWKQLLDEAEGAGTDVEEETRAALEEEAKSGLKRTAREIGEEAKRAGRRRR
                     TEILDLGLELCAAWLRDLAALASGAEEVVFNRDRLDLLRAQSAGIDAARARSGAELVQ
                     QVRRSLDLNVSEELALEALFFRLERLFA"
BASE COUNT          646 a         1638 c         1610 g          659 t
ORIGIN      
        1 cgctgcgcga gcgcacgtcg cggcagatgt cgagcccgct cggtcccggc atcatgatgt
       61 cgaggatcag caggtcgtag ggaatcccgt tgccgagcag ctccgtggcc tcgtcgccgt
      121 cggcggcggc ggtcacctcg aagccctcct gggtcagcgc gtagccgacc gagtcgcgga
      181 ccgagggctc gtcgtcggca atcaggatgc gtcccgtgct catgctgttc gttctccgct
      241 catgcttatg cgaccggggt cgggctgggt tgtgcgacgg gcaggcggac ccagaaggtg
      301 ctgccctcgc cgagggtgga gtcggcgccg atctggccgc ccatcacgtc gaccatgcgc
      361 cgcgcgatcg ccaggccgag cccgaagccc tcctgcttgc gcgactcgct gcgccggtag
      421 aaacgctcga agacgcgctc gatctccgcc gccgggatcc cggtcccggt gtcgattacc
      481 tccacgatga cctcgccctc tccgtcatgc cggccgcgaa cggtaacggt gccgggcggc
      541 ggcgtgttct tgaacgcgtt ggtgaccagg ccgatcagca cctggcgcag cagggtcggg
      601 tcgccggtcg ccgccaggtc gctctcgcag tcgacctcaa gcgccacgtg cttgggttcc
      661 tcgaccgcgg cgacgacgtc gcggatcgcc gccggcacgt cgaccacctc gctctcggcg
      721 gcgccgcgcg actcgacccg ggcgagggtg agcagcgact gggtcagccg gttcatccgc
      781 tcggcgtcgt cggccagccg gttgaggaag tggtcgcggt cgaccgggtc gtttttggcg
      841 ccgccctgca ggacctcgat cgcgccggag atcccggcca gcgggttgcg cagctcgtgc
      901 gcggcgttgg agacgaagtc ggcttcggcg agctcgcggc gcagctcgtc ggtgcggtcg
      961 cggagcaccg cgagcacggc gcgctcgtcg ggcagcgagc gggcggtcat cgcgtaggcg
     1021 tggtcgccga tccgcagcgc cgggtgcgcg gcgaagccga cctcggaggc gcggtgcaac
     1081 tgcggcagga cgacgtcggg cgggcggccg gcggagtcga gcagccgcga ggcagctttg
     1141 ttggagaagc ggatcgggcc ctcgaagtcg accacgatca ccgcgtcggt cagcccgtcg
     1201 aagatcgccg cgagcttgtt gcggtcggag gagagcacgc cgaagctctc cttcagcgag
     1261 gcccgcatcg agtccagggc gcgcgccagg tcgccgatct catcggggcc ggagacgttc
     1321 aggggcgcgt cgaaggagcc gccggccatc tctttggcgg cgcgggcgag gcgcacgacc
     1381 cggtgcgaga tcgcaaccgc gatcaggaaa ccggcgagca ccccgatcat caccgcggcg
     1441 accagcgcct gcacgttgtc gccgaacagg tagacggcgg cggccgtgat caggctgacc
     1501 gagccgaagg cgccggtgag ccagagacgc atgccgatcc gcggcgcgcc gatccgcggc
     1561 gctcccgggc gcggcggggc cggcgtctcg tttgggggac tcatgaaggt ttactaacac
     1621 aatccttcgc tcgccgcgag gaaccagggc ggtgaagtga cctcttcatg ggagaatcga
     1681 cacaaatgcg cacgctcgtc tttattcccg cctggaatga gcaggcttcg atctccgcgg
     1741 tggtcgcgga tgtccgcgag cacctgccga aggccgacat cctcgtcgtc gacgacggct
     1801 cgaccgacgc gacgacccga acggcgcgcg aggccggcgt tctcgtcgcc accctgccct
     1861 tcaaccaggg cctcggagcg gcgctgcaga ccgggtacct gctggccctg cgcgagggct
     1921 acgactgctg cgcccacctc gacgccgacg gtcagcaccc ggccgaggag gtggcgcgcc
     1981 tgctcgccga ggtcgaaggc gaccgcgccg acctcgtgat cggctcccgc tacaccgacc
     2041 ccgacgccga ggccggggtc gacgactacc ggccgacctt ctcgcgccgg atcgggacca
     2101 gcgtcttccg cttcttcctc accctcgcca cccgccagcg cttcaccgac acgaccagcg
     2161 ggatgcgcgc cgccaaccgc aaggtgatgg cgctgttcag cgagcactac tcgcccgact
     2221 tcgccgagat cgagtcgctg cagctggccg tgcgcgaggg cctgcgcgta caggaggtgc
     2281 cggtgcggat gctcgagcgg gtcggcggca gctccttcct caccccgctg aagtcggcct
     2341 tctttatctt caagggcgtc gtcgtcctgc tcgtcggcca gttccggccg cgccgggtgg
     2401 aggcggagcg atgagcctgc cgcttgccgc cagcgacccc aacacggtcc acctcaccgc
     2461 ccagacccgg atcctcgcgg cgatcctcgc gatcatcttc atgttcgcga tcctcgagct
     2521 gatccgccgc gacaaactgc aggagcgcta cagcgtgatc tggtttgtcg ccggcctcgg
     2581 gatgctggcc ggcgccgcct tccccggcct gctcgagctg gtcgccgacg cgatgggggt
     2641 acgcgacacc aacgtcgcgc tgttctcgat cgtgctcttc ttcctgctcg ggctggcgct
     2701 caacttcagc gtgatcatgt cgcgccaggc ggcccagatc acgcgcctgg cccaggaacg
     2761 cgcgctcgag ggcgtgcgcg accaggaggc ggggagacgc aacggaaacg gcgccgagcc
     2821 ggccgtggtc gatcccccag cggtcaaacc gccgggggag tcgccttgac ctatctccgc
     2881 tcgaagctca cctacgccaa cgtcgtcgcg acgctcgcgc tctgcatcgc cgtcggcggc
     2941 gcctccgcct tcgccgctaa ccagctcgcg aagaacagcg tcggcacgaa gcagctgaag
     3001 aagaacgcgg tgaccacggc gaagatcaag aaccgcgcgg tcaccggcgc caagctgaag
     3061 accgcgacca tcggcaccgt gccgagtgcc caggtcgcga acacgctggc cgcaccggaa
     3121 ccgtggcacg aagtcggcaa accgggagag ccctcgttcc tcaacggctg ggaccactac
     3181 ccggggtccc cgctcgtcgc cttctaccgg gatgcgggcg gagtggtgca cctgcgcggc
     3241 gccgtcaccg gagcgaacga ggaaaccgcc gcgttcttcc tgcccgccgg cttccgcccc
     3301 gcggccaccc agagcttcgc gagctggggc gggccacaag cgttgatcgt gattcctgcc
     3361 tccggcgagg tgcgtcccta ctgcggcacc ggtggcgtct gcgcaaccgg cctcgaaggc
     3421 atcagcttcc gcgccgaagg atgagcgcca gctccaacgg ttgctatgcg aagagccgct
     3481 cgaggcgaaa gaacagggcc tctagggcca gctcctcgct gacgttgagg tcgaggctgc
     3541 gacgaacctg ctggaccagc tcagcgccgc tgcgggcgcg ggcggcgtcg attccggcgg
     3601 actgagcgcg caacaggtcg agccggtcgc ggttgaagac gacctcctcg gcgccgctgg
     3661 cgagcgccgc gaggtcgcgc agccaggccg cgcaaagctc gaggccgagg tcgaggatct
     3721 cggtgcggcg acggcggccg gcgcgtttgg cctcctcccc gatctcccgg gcggtccgct
     3781 tcaagcctga tttcgcttcc tcctcgagcg ccgcgcgggt ctcctcttcg acgtcggtgc
     3841 cggccccttc ggcctcgtcg agcagctgct tccagggcgc cgagccaacc tcgtcggcga
     3901 gcgccgcggc caggcagcgc tcggcctcgc cgcgcagttc gcgcccccgc ggggagagca
     3961 gcagccgcgc ccgctccagg tcgcctccgg cgagccgggc ggcggcggcg acctcggcct
     4021 ccgccccact tccggcgagc gcggcttcca gcacctgcgc ggggagcggc gcgaagtcga
     4081 ccggctggca gcgcgatgcg atcgtctcca gcagcgcctc cggctcggag ctgagcagga
     4141 tcaggtggac gtaggcgggc ggctcctcga gcgtcttcag cagcgcgttc tggctctcgt
     4201 cgcgcatcgc ctcggcggcc tcgacgacaa agacccgctt gtcgccttcg aacggccggt
     4261 aggcggcggc gcggatcacc cgctcgcgca cctcgtcgac caggtgctgg gcgccacgcg
     4321 ggacgagcca gacgaggtcg ggatgtggcg aggggtcgag cagggcgcgg cggcgggcgt
     4381 cctcggggtc ggcggcaccg tcggccagga tctccgccgc cagcgcccgc gccgcggcgc
     4441 gcttgccggc gccgcgcggc ccgcggaaga gataggcgtg cgagggcccg ctctccagcg
     4501 ccgcggtcag cgcgatccgg gcgcgcccct ggtgctcggt cgcctcaagc agg
//
LOCUS       DASRIG010000139         7636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_591534, whole genome shotgun sequence.
ACCESSION   DASRIG010000139 DASRIG010000000
VERSION     DASRIG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591534"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFB52_03790"
     CDS             <1..1065
                     /locus_tag="VFB52_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05483.1"
                     /translation="ISNLIPRLSTCGVDVDATHIYWGDPYNETIGRMELSNTRTGDAD
                     ILSERLGIDQALISGVEGACGVAVEGGRVYWTSSDGTIGRANVDGSGIQRDFISGLVD
                     PCGVDVDATHVYWGDAVADAIGRARIDGSEVEAEFIADAEDSCDIAVNGSHLYWSSFD
                     YAIGRADLDGDNPDPGFLPLPGYPCGIAVDPAHLYWTYPMKPGGAQVGSANLDGSAAA
                     PLVVDPDYGADCGIAVDSRVFHPASLPASLPLRFGKVKRQRGGKLLTMEVVVAEGGEL
                     EVVSPALGWSLDKGPAPAPGVAGAFRWKLSLWPGKGRVGKRIRKQLREKGKAPVTLRF
                     AWTQDRHETLDQLKRLAFTR"
     gene            1076..2284
                     /locus_tag="VFB52_03795"
     CDS             1076..2284
                     /locus_tag="VFB52_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZO05484.1"
                     /translation="MRALVISDTHFGAWTGRDVLREEFALERLAPALEGVDELIFLGD
                     LFDFLFGGVEEAVDASDGLLALIAGQLPGKRLVFLAGNHDHHLVHRDEEDRLEAKLAH
                     GTAPPSNGAGPSFFHAFLERKLPGVEVEIAYPTYRFADVLLTHGHYLDPHARLSGSRA
                     DRLLTRTLWAIAAGGPEEPTCVEDYESVTTLLTEWLYTAAQMPHGTHAQQNVFRAAQK
                     AGRVVNAIGAPVRGAKRLGEGLRSRRTGGAPDLPSEAHFDAVVREEAARQAREQPLSG
                     PSWSVPLYPEPTVISRSDASQHALEAFAQVVANLGWDAEAEKIVFAHTHQPLADVRSA
                     SGSKTRYWNTGSWIYEPELGSREAYARYLRYAWPGTAIVIDEDEPQPRLLELLADLNP
                     RNGGAGLPSP"
     gene            2334..3719
                     /locus_tag="VFB52_03800"
     CDS             2334..3719
                     /locus_tag="VFB52_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO05485.1"
                     /translation="MLAPPDPEKLAALRATLGGSVLADGEPGYEDARAVFNAMIEKRP
                     AAIAQCESADDVRAALAFARESGLEVAVRGGGHSVAGAALSEGGLTIDLRRMNAARVD
                     PGARTITVQGGATWADFDSACQPHGLAGTGGRVSTTGVAGLALGGGSGWLERKFGLAC
                     DSLVAAELITADGRSVHASESENPELFWALHGGGGNFGIATALTFRLHELPEFTAALL
                     VWPAADGAGLVRAYRELLERAPDEVGGFLAYITGPEEDFVPATLHGELVCAVLVTYAG
                     PEGKARDAFAPLAALEPAGELVAEMPYAELQSMLDDPPGYRNYWSAEYLAELPDAAVD
                     VFTIAARGIIVPSPTQHILFPWGGAVTSQSAGSPLATRDAPWVVHPLTMWEDPADDER
                     AIAWTRELCAAIAPWSTGATYLNFVGDEGEERILSGYGAANYTRLAAVKAEYDPDNVF
                     HLHHPVRPLTA"
     gene            complement(3744..5894)
                     /locus_tag="VFB52_03805"
     CDS             complement(3744..5894)
                     /locus_tag="VFB52_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HZO05486.1"
                     /translation="MTRIRQQNVTAAQWSANGESLGALDLTLPENEAFGANVFDTAVQ
                     KERLSSDAFEKLQATLKGGGQLDAGLADEVAKAMMEWALENGATHYTHMFQPLTGSTA
                     EKHDSFFEPKRDGSFIADFSGKELIQGEPDASSFPTGGIRATFEARGYTAWDPTSPAF
                     ILENPNGTLLCIPTAFASWSGEALDGKIPLLRSMDALSTAAIKALRLLGDESAEHVFT
                     TVGPEQEYFLIDEQYFYERPDLIATGRTLFGAKPPKGHELDDHYFGSIPERVLAFMME
                     SELEMKKLGIPVKTRHNEVAPAQYEIAPMFENSNVGSDHQQLLMQTLENVARRYGFVC
                     LLHEKPFAGVNGSGKHNNWSMGTDTGVNLLNPGDTPADNIHFLFFCTAVIQAVNKHQG
                     LLRASVANIGQDHRLGANEAPPAIISIFLGAELEKVFEAIATGEGDPNTPASFLDLGS
                     DVLPKLPLDGGDRNRTSPFAFTGNKFEFRALGSSMSLAFPNTVLNTIAAEAIDDLAGK
                     LEEKLSSGTAMPEAVIEIVKESYLANKVVCFGGDNYSEEWHAEAEQRGLKNLKTTPDA
                     LPEVLAPDTVAAFAKYNVLSERELESRYEVWVEQYALRANIEAETAHTIAKTMILPAA
                     LRYLALIGESGVAEGVALEVRENLEALGKSLKALEDGNAYPEDLEGIELAIHARDKQL
                     APMGEVRAAADKLERIVADDLWPLPKYEEMLFIK"
     gene            complement(6083..6607)
                     /locus_tag="VFB52_03810"
     CDS             complement(6083..6607)
                     /locus_tag="VFB52_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05487.1"
                     /translation="MRFSAKFSLLCCLLAAVGVLAASPAAAAVPQATLTKDGRAVAPE
                     SAPDSVKAMIAAANRIRHHPYRWGGGHRNWNSAGYDCSGSLSYVLHAAGLLDYPLDSR
                     GFMRWGGAGGGSWVRIYANHGHVFAVIAGLRWDTSMTDDGDRSGPGWSEEMRETRGFR
                     LRHPLGLLSAVPGS"
     gene            complement(6632..7237)
                     /locus_tag="VFB52_03815"
     CDS             complement(6632..7237)
                     /locus_tag="VFB52_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05488.1"
                     /translation="MSRPTYRRLGVDERRQQLLERGAELFTSHPYDELSMNKIATEVG
                     ISKALLYHYFPSKQAFFEETLSVWAEQLQARTEPDPGRPPLEQLTSSLEAFLAMVEEN
                     AVAYRNLMDSATSAPEIRDLVDEVRQRTAERILEALYPDGAPPKARTAVRGWLWFMDG
                     ACLDWIEHRDLKREELRDLLLGVLMGSLVAADALPDVTQVS"
     gene            7337..>7636
                     /locus_tag="VFB52_03820"
     CDS             7337..>7636
                     /locus_tag="VFB52_03820"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZO05489.1"
                     /translation="MNDEMRTDYEGEPMAPEDRTEKEAPPPLVDTISRLEFETLLGGR
                     VFAWIGGFATLLGVVFFMRIAIGEGWLNEETRTVLAAFGSLALFFAGIWLHERKGH"
BASE COUNT         1116 a         2655 c         2652 g         1213 t
ORIGIN      
        1 atctccaacc tgatcccgcg cctttcgacc tgcggagtcg acgtcgacgc cacccacatc
       61 tactggggcg acccctacaa cgagacgatc ggccgcatgg aactgagcaa cacgcgtacc
      121 ggcgacgccg acatcctcag cgaacgcctg ggcatcgacc aggccctgat cagcggggtt
      181 gagggcgcct gcggggtagc ggtcgagggc ggccgcgtct actggaccag cagcgacggc
      241 actatcggcc gggccaacgt cgacggcagc ggcatccagc gggacttcat ctccgggctc
      301 gtcgatccct gcggcgtcga cgtcgacgcc acccacgtct actggggtga cgcggtcgcc
      361 gacgcgatcg gccgcgcccg gatcgacggc agtgaagtcg aagcggaatt catcgccgac
      421 gccgaggact cctgcgacat cgccgtcaac ggatcgcatc tctactggag cagcttcgac
      481 tacgcgatcg gccgcgccga cctcgacggt gacaaccccg acccgggctt cctgccgctg
      541 cccggctacc cctgcggcat cgccgtcgac cccgcccacc tctactggac ctacccgatg
      601 aagcccggtg gggcccaggt cggcagtgcc aacctcgatg gcagcgccgc cgccccgctc
      661 gtcgtcgacc ccgactacgg agccgattgc gggatcgcgg tcgactcgcg cgtcttccac
      721 cctgcttccc tgccagcctc cctgccgctg cggttcggca aggtgaagcg gcagcggggc
      781 ggcaagctgc tgacgatgga agtcgtcgtc gccgaaggcg gcgaactgga ggtcgtctct
      841 ccggcgctcg gctggagtct cgacaagggg ccggcgccgg cacccggggt ggccggtgcg
      901 ttccgctgga agctcagcct ctggcccggc aagggccgcg tcggcaagcg gatccgcaag
      961 cagctgcggg agaaggggaa ggcgccggtg acgctgcgct tcgcctggac gcaggatcgc
     1021 cacgaaacgc tggaccagct gaagcgtctc gcctttacgc gatgatcgtc cgctgatgcg
     1081 ggccctcgtc atctccgaca cccacttcgg tgcctggacc ggccgcgacg tgctgcgcga
     1141 ggagttcgcg ctcgaacggc tggcgccggc gctggagggc gtcgacgagc tgatcttcct
     1201 cggcgacctc ttcgacttcc tcttcggcgg ggtcgaggag gcggtcgacg cctccgacgg
     1261 gttgctggcg ctgatcgccg ggcagctgcc cggcaagcgc ctcgtcttcc tcgccggcaa
     1321 ccatgaccac cacctcgtcc accgcgacga ggaggaccgg ctggaggcaa agctcgcgca
     1381 cggcaccgct ccgccgtcca acggcgccgg gcccagcttc ttccacgcct tcctcgagcg
     1441 caagctgccg ggagtcgagg tcgagatcgc ctacccgacc taccgcttcg ccgacgtgct
     1501 gctcacccac ggccactacc tcgacccgca cgcgcggctg agtggctcgc gcgccgaccg
     1561 gctgctgacc cggacgctct gggcgatcgc cgccggagga ccggaggagc cgacctgcgt
     1621 cgaggactac gagtcggtga cgacgctgct gaccgagtgg ctgtacaccg ccgcgcagat
     1681 gccgcacggg acgcacgcac agcagaacgt cttccgcgcg gcccagaaag caggccgcgt
     1741 cgtcaacgcc atcggggctc cggtgcgggg ggcgaagcgg ctgggggagg ggctgcgctc
     1801 gcgccgcacc ggcggcgccc cggatctgcc cagcgaagcg cacttcgacg cggtcgtgcg
     1861 cgaggaggcg gcgcggcagg cgcgcgagca gccgctcagc ggcccctcct ggtcggtgcc
     1921 gctctacccg gagccgaccg tgatctcgcg cagcgacgcc tcccagcacg cgctcgaggc
     1981 gttcgcccag gttgtcgcca acctcggttg ggacgccgag gcggagaaaa tcgtcttcgc
     2041 ccacacccac cagccgctcg ccgacgtgcg ctcggccagc ggctcgaaga cgcgctactg
     2101 gaacaccggc tcctggatct acgagcccga gctcggctcg cgcgaggcct acgcgcgcta
     2161 cctgcgctac gcctggccgg gcacggcgat cgtgatcgac gaggacgagc cgcagccgcg
     2221 gctgctcgag ctgctcgccg acctcaaccc ccgcaacggc ggggccggct tgccctcccc
     2281 ctgagggagg actactctcg ggcgaggttc cgttagatcg gaatccttgt ccaatgctcg
     2341 ccccacccga cccggagaag ctggccgctt tgcgcgccac cctcggcggc agcgtgctcg
     2401 ccgacggcga gcccggctac gaggatgcgc gcgcggtctt caacgcgatg atcgagaagc
     2461 ggccggcggc gatcgcccag tgcgagagcg ccgacgacgt ccgcgccgcg ctcgccttcg
     2521 ctcgtgagag tgggctcgag gttgcggttc gcggcggcgg ccacagcgtc gccggcgcgg
     2581 cgctcagcga gggcgggctg acgatcgacc tgcggcggat gaacgcagcc cgggtcgacc
     2641 cgggcgcccg cacgatcacc gtccagggcg gcgccacctg ggccgacttc gactccgcct
     2701 gtcagccgca cggcctcgcc ggcaccggtg gccgcgtctc caccaccggc gtcgccggcc
     2761 tcgcccttgg cggtggctcc ggctggctgg agcgcaagtt cgggctcgcc tgcgacagcc
     2821 tcgttgcggc cgagctgatc accgccgacg gccgctcggt gcacgccagc gagagcgaga
     2881 acccggagct gttctgggcc ctgcacgggg gcggcggcaa cttcgggatc gccaccgcgc
     2941 tcaccttccg cctgcacgag ctacccgagt tcaccgcggc gctgctggtc tggcccgccg
     3001 ccgacggcgc ggggctggtc cgcgcctacc gcgagctgct cgaacgcgcc cccgacgagg
     3061 tcggcgggtt cctcgcctac atcaccggcc cggaagagga cttcgtcccc gcgaccctgc
     3121 acggggagct ggtctgcgcc gtgctcgtca cctacgccgg gcccgagggc aaggcgcgcg
     3181 acgcgttcgc gccgctggcc gcgctcgaac ccgctggcga gctggtcgcc gaaatgccct
     3241 acgccgagct gcagtcgatg ctcgatgacc cgcccggcta ccgcaactac tggtcggccg
     3301 agtacctggc cgagctgccc gacgccgccg tcgacgtctt cacgatcgcg gcgcggggga
     3361 tcatcgtgcc ctcgccgacc cagcacatcc tcttcccctg gggcggcgcc gtgaccagcc
     3421 agtcggccgg ctctccgctc gccactcgcg atgccccctg ggtggtgcac ccgctgacga
     3481 tgtgggagga cccggccgac gacgagcgcg cgatcgcctg gacccgcgag ctctgcgccg
     3541 cgatcgcgcc ctggtcgacc ggcgccacct acctcaactt cgtcggcgac gagggcgaag
     3601 agcgcatcct ctccggctac ggggccgcca actacacccg cctcgccgcg gtcaaggccg
     3661 agtacgaccc cgacaacgtc ttccacctcc accacccggt ccgcccgctg accgcgtagt
     3721 cagcgggcgg agggtggagg gcgctacttg atgaacagca tctcctcgta cttcggcagc
     3781 ggccagaggt cgtcggcgac gatccgctcg agcttgtcgg cggcggcgcg gacctcgccc
     3841 atcggcgcga gctgcttgtc gcgggcgtgg atcgcgagct cgattccctc gaggtcctcc
     3901 gggtaggcgt tgccgtcctc cagggctttc agcgatttgc ccagcgcctc gaggttttcg
     3961 cggacctcga gggcgacgcc ctcggcgacg ccggactcgc cgatcagcgc caggtagcgc
     4021 agcgccgccg gcaggatcat cgtcttcgcg atggtgtgcg cggtctccgc ctcgatgttg
     4081 gcgcggagcg cgtactgctc gacccagacc tcgtagcggg actcgagctc acgctcgctg
     4141 agcacgttgt acttggcgaa cgcggcgacc gtgtccggcg cgagcacctc gggcagcgcg
     4201 tcgggggtgg ttttgaggtt cttcaacccc cgctgctcgg cctcggcgtg ccactcctcg
     4261 gagtagttgt cgccgccgaa gcagacgacc ttgttcgcca ggtaggactc tttgacgatc
     4321 tcgatcacgg cctcgggcat cgccgtgccg gagctcagct tctcctccag cttgccggcg
     4381 aggtcgtcga tcgcctcggc ggcgatcgtg ttcaggaccg tgttcgggaa ggccagcgac
     4441 atgctcgacc ccagcgcccg gaactcgaat ttgttgccgg tgaaggcgaa cggcgaggtc
     4501 cggttgcggt caccgccgtc aagcggcagt ttcggcagca cgtcggagcc gaggtcgagg
     4561 aacgaggccg gcgtgttcgg gtcgccctcg ccggtcgcga tcgcctcgaa cactttttcg
     4621 agttcggcgc cgaggaagat cgagatgatc gccggtggcg cctcgttggc cccgaggcgg
     4681 tggtcctgac cgatgttggc gaccgaggcc cgcagcaggc cctggtgctt gttcaccgcc
     4741 tggattaccg ccgtgcagaa gaacaggaag tgaatgttgt cggctggagt atcacccggg
     4801 ttcagcaggt tgaccccggt gtcggtgccc atcgaccagt tgttgtgctt gccggacccg
     4861 ttcaccccgg cgaacggctt ctcgtgcagg aggcagacga agccgtagcg gcgggcgacg
     4921 ttttccagcg tctgcatcag cagctgctgg tggtcggagc cgacgttcga gttctcgaac
     4981 atcggcgcga tctcgtactg ggccggcgcg acctcgttgt gacgggtctt gaccgggatg
     5041 cccagcttct tcatctccag ttcggactcc atcatgaagg cgagcacccg ctccgggatc
     5101 gagccgaagt agtggtcgtc gagctcgtgg cccttcggcg gcttggcgcc gaacagggtg
     5161 cgcccggtcg cgatcaggtc gggccgctcg tagaagtact gctcgtcgat caggaagtac
     5221 tcctgctcgg gcccgaccgt ggtgaacacg tgctcggcgc tctcgtcgcc aagcagccgc
     5281 agggctttga tcgccgcggt cgagagtgcg tccatcgagc gcagcagcgg gatcttgccg
     5341 tcgagcgcct ctcccgacca ggacgcgaac gcggtcggga tgcagagcag cgtcccgttc
     5401 gggttctcga ggatgaaggc cgggctggtc gggtcccagg cggtgtagcc gcgcgcctcg
     5461 aaggtggcgc ggataccgcc ggtcgggaac gaggaggcat cgggctcgcc ctggatcagc
     5521 tccttgccgg agaaatcggc gatgaacgag ccatcgcgct tgggctcgaa gaacgagtcg
     5581 tgcttctccg ccgtcgagcc ggtcagcggc tggaacatgt gcgtgtagtg ggtggcgccg
     5641 ttctccagcg cccactccat catcgctttg gcgacctcgt cggcaaggcc ggcgtcgagc
     5701 tggccgccgc ccttcagggt cgcctgcagc ttctcgaagg cgtcgctgga gaggcgttct
     5761 ttctggacgg cggtgtcgaa gacgttggcg ccgaaggctt cgttctcggg caaggtcagg
     5821 tcgagcgcgc cgagggactc cccgttggcg ctccactgcg ctgcggtcac gttctgctgg
     5881 cggatacgag tcatcgtatg gtcagttcct cacttgggcg cgcgctcggt gcccgagcgc
     5941 gatcagttgt gtgtctaact ccctatttaa gagccaaaag agcgtaacgc gacgccccca
     6001 gacgggcatt tatacggccg tacagggttt gccagggggt ccttatccgc cctgtaagga
     6061 gtttccgtgc gttatggagg cgctaggagc cggggaccgc gctgagcagc ccgagcgggt
     6121 ggcgcaggcg gaagccgcgc gtctcgcgca tctcttcgct ccagccgggg ccggagcggt
     6181 caccgtcgtc ggtcatcgag gtgtcccagc gcagcccggc gatcactgcg aagacgtgcc
     6241 cgtggttggc gtaaatccgc acccagctgc cgcctccggc gccgccccag cgcatgaacc
     6301 cgcgcgagtc gagcgggtag tcgagcagcc cggcggcgtg gagcacgtag ctgagtgagc
     6361 cggagcagtc gtagccggcc gagttccagt tgcggtgccc gccgccccag cggtaggggt
     6421 ggtggcggat ccggttggcc gcggcgatca tcgccttgac cgagtccggc gccgactccg
     6481 gggcgaccgc gcgaccgtcc ttggtcaggg tcgcctgcgg gaccgcggcc gccgccggtg
     6541 aggcggcgag gacgccgacc gccgcgagca ggcagcagag cagcgagaac ttggccgaga
     6601 accgcatcaa cgggccgtat cggcagtgcg gctaggagac ttgagtgacg tccggcagtg
     6661 cgtcggcggc gaccagcgag cccatcagca ccccgagcag gaggtcgcgc agctcctcgc
     6721 gtttgaggtc gcggtgctcg atccagtcca ggcaggcgcc gtccatgaac cagagccacc
     6781 cgcgcaccgc cgtacgcgcc ttcggcggcg ctccgtccgg gtagagcgct tcgaggattc
     6841 gctccgcggt gcgctggcgg acctcgtcga ccaggtcgcg gatctcgggg gcgctggtcg
     6901 cgctgtccat caggttgcgg taggcgaccg cgttctcctc gaccatcgcc aggaaggcct
     6961 cgaggctgct ggtcagctgc tccagtgggg ggcggccggg gtcgggctcg gtgcgggcct
     7021 gcagctgctc ggcccagacg ctcagggtct cctcgaaaaa agcctgcttg ctggggaaat
     7081 agtggtagag cagcgccttc gagatcccca cctcggtggc gatcttgttc atcgagagct
     7141 cgtcgtaggg gtggctggtg aacagctcgg cgccgcgctc gagcagctgc tggcggcgct
     7201 cgtcgacccc gaggcggcgg taggtgggtc ggctcatcgt ccacattcta ctcgtcttat
     7261 tgaccgatgg ttgacaagag tgttaccgtt gtcgaccgat ggtcaacaag caaccacgaa
     7321 ctggggagtg gaaccgatga acgacgagat gcgaacggac tacgaagggg agccgatggc
     7381 tccggaggat cgaacggaga aggaggcacc gccgccgctc gtggacacga tctcgaggct
     7441 cgagttcgag accttgctcg gcggccgcgt gttcgcttgg atcggcggtt tcgcgaccct
     7501 gctcggggtc gtcttcttca tgcggatcgc gatcggcgag ggctggctca acgaggaaac
     7561 ccgcaccgtg ctcgccgcct tcggctcgct cgcgctgttc ttcgccggga tctggctgca
     7621 cgaacggaaa gggcac
//
LOCUS       DASRIG010000140         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3462243, whole genome shotgun sequence.
ACCESSION   DASRIG010000140 DASRIG010000000
VERSION     DASRIG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462243"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="steA"
                     /locus_tag="VFB52_03825"
     CDS             <1..1004
                     /gene="steA"
                     /locus_tag="VFB52_03825"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZO05490.1"
                     /translation="LIAAGVAAVINASPSSNGKYPNAGPLMLTRAGIRLIDTPDRAPF
                     ELLNDGDRVTIDGGTVRIGDQEVLRGHVLRDAELELQLEEQRERIDEALAEFAENTVA
                     HVREETDLLTGSIDFPPTKAVFRDRHCLIVVRGDRHRRDLKALRAYIRDMRPLIVAVD
                     GGADGVLEAGLKPDLILGDMDSASDQALACGAELIVHAYPDGRAPGAERLAEAGLDYT
                     VVPAAGTSEDVAMLMAYEKGAALIVSVGAHFNLIEFLDRKRGGMSSTFLTRLRIGERL
                     VDAKGVSRLYNPSSTLAPIAFFGVAFLILLTILVITSPALNDVFELFWLKVRIWLGI"
     gene            1039..1905
                     /locus_tag="VFB52_03830"
     CDS             1039..1905
                     /locus_tag="VFB52_03830"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZO05491.1"
                     /translation="MGYSARYHATSLIAVFLALAIGILIGAEFGGDALTGTRKDLERS
                     LVGNLEDAREQADQLSGELGNANEFGERVYPALTRDELDGKRIAILALGGLPSEVTAA
                     VEEGLAPTGADLVGVGVVREPVDVNGMAEDLSKTRFFDLQTNSDQLTALGKGLGRQLV
                     VGGTLPEVVRSHLFSRTSGSFGALDGVIVVRDQPQDMGPVQRANANQFESALMAGVAG
                     TRTAAVGVETIDEEPSSISFFQGNDLSSVDDIDQTAGQLAMVFVMLGAEGSFGVKGSA
                     DRLLPELLSSEE"
     gene            1910..2746
                     /locus_tag="VFB52_03835"
     CDS             1910..2746
                     /locus_tag="VFB52_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05492.1"
                     /translation="MYWVGFPLAIAVALFLVPAGARGLRDAGLVRENYRGAQLAFPLG
                     AVLATAALVSLAPLAFLNDRADLDLLEPDLRQWLPYLLGIAFLGFLDDALGQGEAAAT
                     PRGWRGHWRALNEGRLSTGAIKAIGALALAAYVVSGRGLEDWRYVADVLLLLLATNLG
                     NLLDLRPGRAEKGLALLGLGLCLGAWTFAPLELLGIFAGPVLVGAWLTLRETAMLGDT
                     GSNLIGAIAGVWLLTTLGGDGRLIALAVVLAITVYGELRSISATIDSVPPLRWLDSLG
                     RR"
     gene            2743..4431
                     /locus_tag="VFB52_03840"
     CDS             2743..4431
                     /locus_tag="VFB52_03840"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZO05493.1"
                     /translation="MSPSHKGDTKFIFVTGGVVSALGKGITAASIGRLLVSRGYRVQL
                     QKFDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDENTSRASNVTAGAVY
                     NSVIRRERRGDYLGATVQVIPHITNEIKQKILIVAESQAVDFVITEIGGTVGDIESLP
                     FLEAIRQLYTDLTPKRAMFIHLTLVPYVGHAGELKTKPTQHSVNELRRIGIQPHALVC
                     RSEGRLDREIREKIALFASLPVEAVISAHDVDNIYKVPLVFRAEGVDDTVLDHFGMEA
                     PSPDLAEWEAMVRRADAASGKVRIAIVGKYVQLADAYKSVIEAIEHGGIHHGVETEIE
                     LVDSENLDPEQLEGMDGILIPGGFGERGIEGKVQAARIARERSIPYFGICLGMQIAVV
                     EFARTVVGMEGANSAEFDPETPYPVVDLLPEQKEVSDMGGTMRLGADPVKLHEGTKAR
                     EIFGEAVIYKRHRHRYEVNNQLRRRLEDEGLVCGGTSPDERLVEMIELPDHPFFVASQ
                     YHPEFNSRPNRPEPLFREFVGAAARHAGERPADGGDLEDVEDTADSDVGVRRSA"
     gene            4454..5599
                     /locus_tag="VFB52_03845"
     CDS             4454..5599
                     /locus_tag="VFB52_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO05494.1"
                     /translation="MEGNQRLFDTFVRLCEIRSPTGEEREIADTLAAELRALGLEVEE
                     DDAAGPAEAGAGNLLARLPGAGEEWVMFCAHIDTVPHKGRVEVVDDEGVFRSRGETIL
                     GADNKAAVAVFMELVARATERKSEVGIELLLTVAEEQGLRGAKVFDTSKLRAPVGFVL
                     DHAGPVGEVIVQTPTQQKIRADFHGVEAHAGIRPEDGNSAIAAAAAAISRMELGRLDE
                     GTTANVGLISGGTSGNVVPGHCWIHAETRSLDPERAAEIAGKMSDACAWGASEHGCDV
                     DVRLEELFRGYELPKDSPALGFAEAGLRGAGLEPARVAIGGGSDVNAFRRAGHDSVLL
                     SNGTDMVHTEHEFVPAANLVKMLEVCEGIVAAAANGASAPDGAASEA"
     gene            5596..>5963
                     /locus_tag="VFB52_03850"
     CDS             5596..>5963
                     /locus_tag="VFB52_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HZO05495.1"
                     /translation="MSGRLDLRRGVVVSVEPLQVEIEGERRPAWADAVLLGEMREGDE
                     VVVNVAALDLGLGSGGFDVVHVNLTRGLEGTDRRGPEHVLKLNYTSLQHPVAPVEETV
                     AEESRAMRGKAQPRPPAPADL"
BASE COUNT          898 a         2128 c         2072 g          865 t
ORIGIN      
        1 agctgatcgc cgccggcgtc gccgccgtga tcaacgcctc gccttcctcc aacggcaagt
       61 acccgaacgc ggggccgctg atgctgaccc gggccggcat ccgcctgatc gacacgcccg
      121 accgcgcccc cttcgagctg ctcaacgacg gcgaccgagt gacgatcgac ggcggcacgg
      181 tgcggatcgg cgaccaggag gtgctgcgcg gccacgtgct gcgcgacgcc gagctggagc
      241 tgcagctcga agagcagcgc gagcggatcg acgaggcgtt ggcggagttt gccgagaaca
      301 cggtcgccca cgtgcgcgaa gagaccgacc tgctgaccgg cagcatcgac ttcccgccga
      361 ccaaggcggt gttccgcgac cgccactgcc tgatcgttgt ccgcggcgac cgccaccggc
      421 gcgacctgaa ggcgctgcgc gcctacatcc gcgacatgcg gccgctgatc gtcgccgtcg
      481 acggtggcgc cgacggggtg ctggaagcgg ggctgaagcc cgacctgatc ctcggcgaca
      541 tggactccgc ctccgaccag gcgctcgcct gcggcgccga gctgatcgtc cacgcctacc
      601 ccgacggccg cgcccccggc gccgagcggc tcgccgaggc cgggctcgac tacaccgtcg
      661 tccccgccgc cgggaccagc gaggacgtgg cgatgctgat ggcctacgag aagggcgccg
      721 ccctgatcgt cagcgtcggc gcccacttca acctgatcga gttcctcgac cgcaagcgcg
      781 gcggcatgtc ctcgaccttc ctcacccggc tgcggatcgg cgagcgcctg gtcgacgcca
      841 agggcgtcag ccgtctctac aacccgagct cgaccctcgc cccgatcgcc ttcttcgggg
      901 tcgccttcct gatcctgctg acgatcctcg tgatcacctc gccggcgctc aacgacgtct
      961 tcgagctgtt ctggctgaag gtgcgcatct ggttggggat ataggtccgg gcacggacct
     1021 tctagccttc ggcggctgat gggctacagc gcgcgctacc acgcaacctc gctgatcgcc
     1081 gtcttcctcg cgctcgcgat cgggatcctg atcggcgccg agttcggcgg cgacgcgttg
     1141 accgggaccc gcaaggacct cgagcgcagc ctggtcggaa acctcgagga cgcgcgcgaa
     1201 caggccgacc agctgagcgg cgaactcggc aacgccaacg agttcggcga gcgggtctac
     1261 cccgcgctga cccgcgacga actggacggc aaacggatcg cgatcctcgc cctcggcggc
     1321 ctgcccagcg aagtgaccgc cgcggtcgag gagggcctgg cgccgaccgg ggcggacctg
     1381 gtcggggtcg gtgtggtgcg cgagccggtc gacgtcaacg ggatggccga ggacctctcg
     1441 aaaacccgct tcttcgacct gcagacgaac tccgaccagc tgaccgcgct cggcaagggc
     1501 ctgggacgcc agctggtggt cggcggcacc ttgccagagg tcgtgcgcag ccacctcttc
     1561 tcgcgcacca gcggcagctt cggtgccctc gacggcgtga tcgtcgtccg tgatcagccg
     1621 caggacatgg gcccggtgca gcgcgccaac gcgaaccagt tcgagtcggc gctgatggcc
     1681 ggcgtcgccg gcacccgcac ggccgcggtc ggggtcgaga cgatcgacga ggaaccgtcc
     1741 tcgatctcct tcttccaggg caacgacctc tccagcgtcg acgacatcga ccagaccgcc
     1801 ggccagctgg cgatggtctt cgtgatgctc ggcgccgagg gtagcttcgg ggtcaaaggc
     1861 agcgccgacc gcctgctgcc cgagctgctg tcctccgagg agtagcggcg tgtactgggt
     1921 cggcttcccc ctggcgatcg cggtcgcgct gttcctggtg ccggcggggg cgcgggggct
     1981 gcgcgacgcc gggctggtgc gggagaacta ccgcggcgcg cagctcgcct tcccgctcgg
     2041 ggcggtgctg gcgactgcgg cgctggtctc gctggcgccg ctcgccttcc tcaacgaccg
     2101 cgccgacctc gacctgctgg agcccgatct gcgccagtgg ctgccctacc tgctcgggat
     2161 cgccttcctc ggcttcctcg acgacgcgct cgggcagggg gaagcggcgg cgaccccgcg
     2221 cggctggcgc gggcactggc gggcgctgaa cgaagggcgg ctctcgaccg gggcgatcaa
     2281 ggcgatcggg gcgctggcgc tggcggcgta cgtggtctcc gggcgcggcc tggaggactg
     2341 gcgctacgtc gccgacgtgc tcctcctgct gctcgcgacc aacctcggca acctgctcga
     2401 cctgcggccg gggcgggcgg agaaggggct ggcgctgctc gggctcgggc tctgcctcgg
     2461 cgcctggacc ttcgccccgc tcgagctgct ggggatcttc gccgggccgg tcctggtcgg
     2521 cgcctggctg accctgcgcg agacggcgat gctcggcgac accggctcga acctgatcgg
     2581 ggcgatcgcc ggggtctggt tgctgaccac tttgggcggc gacggtcgcc tgatcgcgct
     2641 cgcggtggtt ctcgcgatca ccgtttacgg ggagttgcgg tctatttcgg cgacgatcga
     2701 ttcggttccg cccctgcgct ggctagactc gctcggtaga agatgagtcc atcccacaaa
     2761 ggcgacacca agttcatttt cgtcaccggc ggggtggttt cggcacttgg taaggggatc
     2821 accgcggcct cgatcggccg cctgctcgtc tcccgtggct accgggtcca gctgcagaag
     2881 ttcgacccct acatcaacgt cgacccgggg acgatgagcc cgttccagca cggcgaggtg
     2941 ttcgtcaccg aggacggggc cgagaccgac ctcgatctcg gtcactacga gcgcttcacc
     3001 gacgagaaca cctcgcgcgc ctccaacgtc accgccggcg ccgtctacaa ctcggtgatc
     3061 cggcgcgagc gccgcggcga ttacctcggc gccaccgtgc aggtgattcc ccacatcacc
     3121 aacgagatca agcagaaaat cctcatcgtc gccgagtcac aggccgttga cttcgtaatc
     3181 accgagatcg gcggcacggt cggcgacatc gagtcgctgc ccttcctcga ggcgatccgc
     3241 cagctctaca ccgacctgac cccgaaaagg gcgatgttca tccacctgac gctggtgccc
     3301 tacgtcggcc acgcgggcga gctgaagacg aagccgaccc agcactcggt caacgagctg
     3361 cgccggatcg ggatccagcc gcacgcgctg gtctgccgct ccgaaggccg cctcgaccgc
     3421 gagatccgcg agaagatcgc gctcttcgcc agccttcccg tggaagcggt gatctccgcc
     3481 cacgacgtcg acaacatcta caaggtgccg ctcgtcttcc gcgccgaggg agtcgacgac
     3541 accgtcctcg accacttcgg gatggaggcg ccctcgcccg acctcgccga gtgggaggcg
     3601 atggtgcgcc gcgccgacgc cgccagcggc aaggtgcgga tcgcgatcgt cggcaagtac
     3661 gtgcagctcg ccgacgccta caagtcggtg atcgaggcga tcgagcacgg cggcatccac
     3721 cacggggtgg aaaccgagat cgagctggtc gactccgaga acctcgaccc cgagcagctc
     3781 gagggcatgg acgggatcct gatccccggc ggcttcggcg agcgtggcat cgagggcaag
     3841 gtgcaggcgg cgcggatcgc tcgcgagcgc tcgattcctt atttcggcat ctgcctcggg
     3901 atgcagatcg cggtggtcga gttcgcgcgc accgtggtcg ggatggaggg cgccaactcg
     3961 gctgagttcg accccgagac gccctacccg gttgtcgacc tgctgcccga gcagaaggaa
     4021 gtctccgaca tgggcgggac gatgcgactc ggtgccgacc cggtgaaact gcacgagggc
     4081 actaaagccc gcgagatctt cggcgaggcc gtcatctaca agcgccaccg ccaccgttac
     4141 gaggtcaaca accagctgcg ccgccggctc gaagacgagg gcctggtctg cggcggcacc
     4201 tcgcccgacg agcgcctggt cgagatgatc gagctgcccg accacccgtt cttcgtcgcc
     4261 tcgcagtacc accccgagtt caactcgcgc ccgaaccggc ccgagccgct gttccgcgag
     4321 ttcgtcggcg ccgccgctcg ccacgctggc gaacggccgg ctgacggggg cgacctcgag
     4381 gacgtcgagg acaccgccga cagcgacgtc ggcgtccgcc gctcggcctg agcagccggg
     4441 tagccttccg cgcatggagg ggaaccagag gctgttcgac accttcgtcc ggctctgcga
     4501 gatccgcagc ccgaccggcg aggagcgcga gatcgccgac acgctggcgg ccgaactgcg
     4561 cgcgctcggg ctcgaggtcg aggaggacga cgcggccggt ccggccgaag ccggcgccgg
     4621 caacctgctg gcgcggctgc ccggggcagg cgaggagtgg gtgatgttct gcgcccacat
     4681 cgacaccgtc ccgcacaagg gccgggtcga ggtggtcgac gacgagggcg tcttccgcag
     4741 ccgcggcgag acgattctcg gcgccgacaa caaggccgcg gtcgcggtct tcatggagct
     4801 ggtcgcgcgc gccaccgaac gcaaatccga ggttgggatc gagctgctgc tgaccgtcgc
     4861 cgaggagcag gggctgcgcg gcgccaaggt cttcgacacc tccaagctgc gcgccccggt
     4921 tggctttgtg ctcgaccacg ccgggccggt cggcgaggtg atcgtgcaga cgccgaccca
     4981 gcagaagatc cgcgccgact tccacggcgt cgaggcccac gcggggattc gccccgagga
     5041 cggcaacagc gcgatcgccg ccgccgcggc cgcgatctcg cggatggagc tcggccggct
     5101 cgacgagggc acgaccgcca atgtcggcct gatctccggc ggcacctccg gcaacgtcgt
     5161 ccccggccac tgctggatcc acgccgagac ccgcagcctc gaccccgagc gggcggcgga
     5221 gatcgccggc aagatgtccg acgcctgcgc ctggggcgcc agcgagcacg gctgcgacgt
     5281 cgacgtccgg ctcgaagagc tgttccgcgg ctacgaactg ccgaaggact cgccggcgct
     5341 cggcttcgcc gaggcgggcc tgcgcggcgc cggcctcgag ccggcccggg tggcgatcgg
     5401 cggcggcagc gacgtcaacg ccttccggcg ggccggccac gactcggtgc tgctctccaa
     5461 cggcaccgac atggtccaca cggaacacga gttcgtgccc gccgccaacc tggtgaagat
     5521 gctcgaggtc tgcgagggca tcgtcgccgc cgcggcgaac ggggcgagcg cgcccgacgg
     5581 ggccgcgagc gaggcgtgag cggccgcctc gacctgcgcc gcggcgtcgt cgtatcggtc
     5641 gagccgctgc aggtagagat cgagggcgag cgccgtcccg cctgggccga cgcggtcctg
     5701 ctcggcgaga tgcgcgaggg cgacgaggtc gtcgtcaacg tcgccgcgct cgacctcggg
     5761 ctcggctccg gcggcttcga cgtcgtccac gttaacctga cccgcggact ggagggcact
     5821 gaccgccgcg gtcccgagca cgtgctcaag ctcaactaca cctcgctcca gcatccggtc
     5881 gcgcctgtgg aggagaccgt ggctgaggaa agccgggcta tgcgcggcaa agctcagcca
     5941 cgcccgccgg cgccggctga tct
//
LOCUS       DASRIG010000141        13187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2682555, whole genome shotgun sequence.
ACCESSION   DASRIG010000141 DASRIG010000000
VERSION     DASRIG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682555"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /gene="gyrA"
                     /locus_tag="VFB52_03855"
     CDS             complement(<1..2373)
                     /gene="gyrA"
                     /locus_tag="VFB52_03855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZO05496.1"
                     /translation="MALDALTGNIEERGLEQEMRSSYLDYAMSVIVGRALPDVRDGLK
                     PVHRRVLYAMHDIGLQPTRPYRKCAFIVGEVMGKYHPHGDSAIYDTLVRMAQDFSLRY
                     MLVDGQGNFGSIDDDPAAAMRYTEAKLDRLATELLRDIDADTVDFGPNYDESTQEPQV
                     LPARFPNLLVNGTSGIAVGMATNIPPHNLREVAAAVAAYIDDPGIDLAGLMEHIKGPD
                     FPGGGLMSREGIRDAYASGRGSIRVRARAHVEQLKGGREAIVVTQLPFMVKKGGDGGL
                     ITKIADLVRDKKLEGISALRDESDERSGMRLVIELKRGGPPAKVVLNNLYKKTAMQST
                     FGANMVALVDGVPRTLSLLELIEHYVEHQREVVTRRTQFELRLAEARAHILEGLLIAL
                     DNLDAVIKLIRASSDPDAARDGLIEKFELSREQAQAILDMRLQRLTALEADKVRAEHA
                     DLMERIGELREILGDEAKVMALVKDELAEIVESYGDERRTELAHFEGDVGIEDMIADQ
                     QMVISLTASGYVKRLPLATYRQQHRGGVGVMGMNLKEDDYIAHLHICSTHDYLLFFTN
                     RGKVYRLKVYELPEGQRTAKGSSLRNLLPLREGEKVMAVIPTRDFKENKYLVFATADG
                     LVKKTEFLAYNTPIRADGIIAIKVRDGDELVQVRLTSGEDDILMVSHSGHASRFSEEL
                     VRPMGRDTSGIKGMNVKAKDNRVLAMDIARNDTELFVVTENGYGKRTPVSDYPVKGRG
                     TKGVLTAKLTEKKGGLAGALIVAEHQELLFISQNGMVQRTSVSGISQMGRS"
     gene            complement(2401..4815)
                     /gene="gyrB"
                     /locus_tag="VFB52_03860"
     CDS             complement(2401..4815)
                     /gene="gyrB"
                     /locus_tag="VFB52_03860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZO05497.1"
                     /translation="MPDKKAKGATSDSYGAADITVLEGLEAVRKRPGMYIGSTGPRGL
                     HHLVYEIVDNSVDEALAGHCDTVRVVLHPDNSCTVADNGRGIPVETMEKEGRPAAEVV
                     LTVLHAGGKFGEGGGYKVSGGLHGVGSSVVNALSERLHLQIERDGFVWSQDYERGKPQ
                     TKLEKGEKSNAHGTTITFLPDLEIFEEIGFDFETLAERLRETAFLTRGLKIELVDERG
                     ETNSVTFQYDGGIEDFVRHLNANKESLHKKPIYFEGDKDDGEVEIAMQWNNSYQESIF
                     SFANNINTHEGGTHMSGFRAALTRTLNAYARNKGLLKEKDDNLAGEDVREGLTAVISV
                     KLHDPQFEGQTKTKLGNPPIKGLVEETVNRKLAEFLEENPPEARRIISKTIDAARARA
                     AARKARDLTRKSALENSTLPGKLADCTVKDPSLAEIFVVEGDSAGGSAKQARDRNTQA
                     VLPLRGKIINVEKSRIDKVLANNEIQALITAIGTGVHEEFDITEARYHKIVMATDADV
                     DGAHIRTLVLTFLYRQMPELISAGYVYIAKPPLYRVKQGNQETYLERESELEELLLRD
                     KLEEFELLDATGEKRKLSPSRWQAYIRRSKEYEGWGSSLQADFGHEVVRFLEESSLLD
                     EQVATVAAARDVLQRGGEENEPFETEVLQSDDNIVVVKAVERKSGLARTHRLPAELFQ
                     HHDYRKFAEVHAALLKQVGRPPFEVSRGGKRSVALSFEALRGAVLELARQGVQLTRFK
                     GLGEMNAKQLRETTMDPASRTLARVTLEDATAADTIFSMLMGDQVAPRREFIETHAKE
                     VKFLDV"
     gene            complement(4970..5278)
                     /locus_tag="VFB52_03865"
     CDS             complement(4970..5278)
                     /locus_tag="VFB52_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZO05498.1"
                     /translation="MSRRRAPRQASEAFRAARAMVAPQTGLASVQGAWAEAVGPQLAA
                     VASPVSERAGTLTIEVTDSVWAQELDLMQEQLLERLRAVLGESAPKALKFRLASDTVS
                     "
     gene            complement(5275..6372)
                     /gene="recF"
                     /locus_tag="VFB52_03870"
     CDS             complement(5275..6372)
                     /gene="recF"
                     /locus_tag="VFB52_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HZO05499.1"
                     /translation="MLVTAIEARPLRSLDRARVELGVGITSVVGPNGTGKTNLVEALY
                     FALTGRSFRTSDRRDLIPFGGSLARAEATIRDEDGLERRLLASVSRSEGRRHLLDGNA
                     TDPATLARSRPPVAVFAPDRLSLVKGPPAERRAHLDGFVTARWPYRAELRKRLGQALA
                     QRNALLARLAAGYGNPADLDVWDAGLAEAAAPLVAAREEAVAELAEPFAEAASELGLD
                     GGATLSYAPRAAGSAEEIRAGLQERREQDVRMGRSSWGPHLDELKLETGGRSLRKYGS
                     QGQQRAALLALLFAEREALLLARRVTPLLLLDDVMSELDPGRRERLVARLSDGEGQVL
                     ITAADEESLPAPALESVIRMPPPELAEAAAA"
     gene            complement(6385..7494)
                     /gene="dnaN"
                     /locus_tag="VFB52_03875"
     CDS             complement(6385..7494)
                     /gene="dnaN"
                     /locus_tag="VFB52_03875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZO05500.1"
                     /translation="MKLTTNREELVSKLSIVSRAVSTRAATQALSGVLLSASGEKLTL
                     AATDLDLGLQTDLDAVVEGEGTVLLPGRLLAEVARSLADSKVEIESREAQQDVEIRSG
                     SSSFHLRTLPAEDFPKLPSAGEQSPLKIPAKALEASIDLVARAASRDDMRPVLTGVFV
                     SAAGKEMTMVATDSYRLAVKRTELEAELGGEIEANIPARALRELGRILSAEGVEEAEI
                     WLQTNQAVFAAGSITLTTRLIEGQFPNFRQLLPESYEHDVRLPRSDFLDVARRVSQLA
                     QRNAPLRLSFSSGELTVAAETPDVGDATETMPASFEGETLEIGFNPEFLKEGIESVDG
                     DEILVRLISPLRPGLLQPVEGEDFRYLVMPIRLNV"
     gene            complement(7640..8986)
                     /gene="dnaA"
                     /locus_tag="VFB52_03880"
     CDS             complement(7640..8986)
                     /gene="dnaA"
                     /locus_tag="VFB52_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZO05501.1"
                     /translation="MVADRVPNDPSSQDLDALWRATQERLRASVPESTYRIWFEPLQV
                     AGATDDTLYLTAREERVRAWAERRYSSLIAEALVDAGTPLCNVSFSSPRGGSDPTTGP
                     GGVQLNPNYTFERFVIGPGNRLAHGAALAVAEAPSEAYNPLFLHGPPGLGKTHLLAAI
                     ANYLQANAPALSVRYTTAESFTNEFVIALKSTGAEAFKTRYRDIDVLLIDDVQFLEGK
                     PATEEEFFHTFNALYESGSQLVLSADRIPSELSTLASRLRDRFEWGLTVAMEPPNLTT
                     RLTVLRRLVEEAGVEIADSGALSELAHRIDANVRQLHGALTRVLAHASLMARPLSSEL
                     IAEVIPKSTRSAEATSAEEIQQRVAERFGISRAELVGSSRAATPLRARQVAIFLTREM
                     TDLSLPQIGRLYGGRDHSTVLNSLRRIEAGIGDDEELAARVAELRGAIHNPTRDPS"
     gene            9086..9220
                     /gene="rpmH"
                     /locus_tag="VFB52_03885"
     CDS             9086..9220
                     /gene="rpmH"
                     /locus_tag="VFB52_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZO05502.1"
                     /translation="MKRTYQPKKRKRAKTHGFRARMSTRGGRAILKRRRAKGRKRLTP
                     "
     gene            9223..9591
                     /gene="rnpA"
                     /locus_tag="VFB52_03890"
     CDS             9223..9591
                     /gene="rnpA"
                     /locus_tag="VFB52_03890"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZO05503.1"
                     /translation="MASKRRRLSRSGEFDRVYRDGSSHATRYLVLYTFPRRDESGDDV
                     RLGVSVSRKVGGAVERNKAKRALREAFWSLSDRLPEGHDFVVVARGEINELVDRDGTA
                     GVAKSLEEALAAATPGKRST"
     gene            9588..9863
                     /gene="yidD"
                     /locus_tag="VFB52_03895"
     CDS             9588..9863
                     /gene="yidD"
                     /locus_tag="VFB52_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZO05504.1"
                     /translation="MKRVALAPIRAYQRWISPSRPRRCRYEPTCSAYAVEAVERFGVV
                     RGMILAAWRLLRCNPFNHGGFDPVPKRFTLRGLGHVHPAEYHGEAQV"
     gene            9863..10672
                     /locus_tag="VFB52_03900"
     CDS             9863..10672
                     /locus_tag="VFB52_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZO05505.1"
                     /translation="MSLPFGFQLAPQFLVDIADEVLVFFHDSGLSWGMSIVALTVCMR
                     TLLIPLTYKQLKGMRAMQALQPQIKELQEKYKNDKQRMQQEMMRFYKENKVNPFASCI
                     PLLAQLPVFITLFYVLQHDLRFDICGQTAETCGEFAVTQGHSAGFFGESFFFINDLTS
                     KATGSELIVLLVIYVGTQLISGMVMAVTADKSQRMLMFLLPLFFVPFILNFPAGLILY
                     WITTNFWTIGQSFVVKKLIPVPVAATPAGATAIKEAKPPPKPPKKKKRKRR"
     gene            complement(10707..11384)
                     /gene="rsmG"
                     /locus_tag="VFB52_03905"
     CDS             complement(10707..11384)
                     /gene="rsmG"
                     /locus_tag="VFB52_03905"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZO05506.1"
                     /translation="MSDGPPALDEAGRRALETVLELLAEERASVSSVVDKRAWKVHVE
                     DSLTGLEVPELREAKRIADVGAGAGFPGLVLAVALPGARVDLIESVSRKCEFMQRAIG
                     AAGIANASVLNLRSEDWAAGEGNEAYDAVTARAVARLSTLAELASPLLQEGGHLVAWK
                     GKRDAEEEAQLERAAESLAMAPGQILDVGHRAGSEHRHLHILTKSGPTPANLPRRPGM
                     AKKRPKG"
     gene            complement(11381..11863)
                     /gene="jag"
                     /locus_tag="VFB52_03910"
     CDS             complement(11381..11863)
                     /gene="jag"
                     /locus_tag="VFB52_03910"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZO05507.1"
                     /translation="MSETFEWPDAPADRARELVEGVLDELDLDGEVEVEEDDDVISIT
                     VEGDADYGLLIGKRGQTIDALQLLCYQAAFRGLRERKRVIVDAAGYRERRRETLEGRA
                     DRAAEQALSNNRVVEMDPMSAQERRVVHERLKDRAGVETYSEGDEPHRCVVVAPLVSE
                     "
     gene            11920..12678
                     /locus_tag="VFB52_03915"
     CDS             11920..12678
                     /locus_tag="VFB52_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZO05508.1"
                     /translation="MGKVYAVANQKGGVGKTTSAVNLASCAAAEGQQTLLVDLDPQGN
                     ATTALGGEKEMHPSSYDCLTGDTSVAEAARPAGPDNLWLVPASRDLAGAAVELPRIEG
                     SETRLRERLGPVRERFAVTLLDCPPSLGPVSVNALTAADRVIVPVQAEYLALEGLVQF
                     LETLGLIRQQLNPQLEVAGLLITMYDDRTRLAIDVESELRRHFGEMVFETVIPRSVRV
                     AESPSYGLPVTEHAPGSRGAVAYQALSKELISRG"
     gene            12671..>13187
                     /locus_tag="VFB52_03920"
     CDS             12671..>13187
                     /locus_tag="VFB52_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZO05509.1"
                     /translation="MASGTKRGIGRGLAAILPEVETSSAGELRELPVGLIKPNPNQPR
                     TKFDPEALAALAASIEATGVVQPLLVRPLPDGSFELVAGERRWRAAQQANLDKVPAVI
                     RDQEEAERLQAALIENMVREDLNPVEEAKACAALIEDLGLTKEELAKRVGRSRPAVSN
                     LIRLLELPDELL"
BASE COUNT         1941 a         4399 c         4566 g         2281 t
ORIGIN      
        1 ggaacggccc atctgcgaga tgccgctgac cgaggtgcgc tggaccatgc cgttctggga
       61 gatgaagagc agctcctggt gttcggcgac gatcagggcg ccggcgaggc cgcccttctt
      121 ctcggtcagc ttcgcggtca gcacgccttt cgtcccgcgg cccttgactg ggtagtcgga
      181 gaccggggtg cgcttgccgt agccgttctc ggtgacgacg aacagttcgg tgtcgttgcg
      241 ggcgatgtcc atcgccagca cgcggttgtc tttcgctttg acgttcatgc ctttgatccc
      301 gctggtgtcg cgtcccatcg gccgcaccag ctcctcggag aagcgcgacg cgtggccgga
      361 gtgggagacc atcaggatgt cgtcttcacc cgaggtgagc cgcacctgga ccagctcgtc
      421 accgtcgcgg accttgatcg cgatgatccc gtcagcgcgg atcggcgtgt tgtaggcgag
      481 gaactcggtt ttcttgacca ggccgtcggc ggtcgcgaag acgaggtact tgttctcttt
      541 gaagtcgcgg gtcgggatca cggccatcac tttctcgccc tcgcgcagcg gcagcaggtt
      601 gcgcagcgag ctgccttttg cggtgcgctg gccctccggc agctcgtaga ccttgagccg
      661 gtagaccttg ccgcggttgg tgaagaagag caggtagtcg tgggtcgagc agatgtgcag
      721 gtgggcgatg tagtcgtcct ctttgaggtt catccccatc accccgacgc cgccgcgatg
      781 ctgctggcgg taggtcgcca gcggcagtcg cttcacgtag ccggaggcgg tcagcgagat
      841 caccatctgc tggtcggcga tcatgtcctc gatcccgacg tcgccctcga agtgggccag
      901 ctcggtgcgc cgctcgtcgc cgtaggactc gacgatttcg gcgagctcgt ccttgaccag
      961 cgccatcact ttcgcttcgt caccgaggat ctcccgcagc tcgccgatcc gctccatcag
     1021 gtcggcgtgc tcggcccgga ctttgtcggc ctccagcgcg gtgaggcgct gtaggcgcat
     1081 gtcgaggatc gcctgcgcct gctcgcgtga gagctcgaat ttctcgatca ggccgtcgcg
     1141 cgcggcgtcc gggtcgctcg aagcgcggat cagtttgatc accgcgtcga ggttgtcgag
     1201 cgcgatcagc aggccctcga ggatgtgagc gcgggcctcg gcgaggcgca gttcgaactg
     1261 cgtgcggcgg gtgacgacct cgcgctggtg ctcgacgtag tgctcgatca gctcgagcag
     1321 gctgagggtc cgcggcaccc cgtcgaccag cgccaccatg tttgcgccga aggtcgactg
     1381 catcgcggtt tttttgtaga ggttgttgag aacgactttc gccggcgggc cgccgcgttt
     1441 gagctcgatc accaggcgca tcccggagcg ttcgtcgctc tcgtcgcgca gggcggagat
     1501 gccctcgagt tttttgtcgc gcacgaggtc ggcgatcttg gtgatcaggc cgccgtcgcc
     1561 gcccttcttg accatgaagg ggagctgggt gacgacgatc gcctcgcgac ctcccttcag
     1621 ctgctcgacg tgggcgcggg cgcggacccg gatgctgccg cggccagagg cgtacgcgtc
     1681 gcggatcccc tcgcggctca tcaggccgcc accggggaag tcggggccct tgatgtgctc
     1741 catcagcccg gccaggtcga tgcccgggtc gtcgatgtag gcggcgaccg cggcggcaac
     1801 ctcgcgcagg ttgtgcggcg ggatgttggt cgccatcccg accgcgatcc cggaggtgcc
     1861 gttgaccagc aggttcggga agcgcgccgg caacacctgc ggctcctggg tcgattcgtc
     1921 gtagttgggg ccgaagtcga ccgtgtcggc gtcgatgtcg cggagcagct cggtcgcgag
     1981 gcggtcgagt ttcgcctccg tgtaccgcat cgcggccgcc gggtcgtcgt cgatcgagcc
     2041 gaagttgccc tggccgtcga ccagcatgta gcgcagggag aagtcctgcg ccatgcggac
     2101 cagcgtgtcg tagatcgccg agtcgccatg ggggtggtac ttgcccatga cctcgccgac
     2161 gatgaaggcg cacttgcggt acggccgggt cggctgcagg ccgatgtcgt gcatcgcgta
     2221 gagcacgcga cggtgcacgg gcttcaaccc gtcgcggacg tcgggcaggg cgcggccgac
     2281 gatcacgctc atcgcgtaat cgaggtacga cgagcgcatc tcctgctcta gcccgcgctc
     2341 ctcaatgttg ccggtcaggg cgtcaagcgc catcagcggg ccctcccgtc gccagtcgcc
     2401 ttagacatca aggaacttca cctctttggc gtgggtctcg atgaactctc tgcggggggc
     2461 gacctggtcg cccatcagca tcgagaagat cgtgtcggcg gcggtcgcgt cttcgagcgt
     2521 cacccgggcc agcgtgcgcg aggccgggtc catcgtcgtc tcgcgcagct gtttcgcgtt
     2581 catctcgccc aggcccttga accgggtcag ctgaacgccc tggcgggcaa gctccagcac
     2641 cgcgccgcgc aacgcctcga aggagagcgc gacgctgcgt tttccaccgc gactgacctc
     2701 gaagggtggg cggccgacct gtttgagcag cgccgcgtgg acctcagcga acttgcggta
     2761 gtcgtgatgc tggaacagct ccgcgggcaa gcggtgggtc cgggccaggc cgctcttgcg
     2821 ttccacggcc ttgaccacca cgatgttgtc gtcgctctgc agcacctcgg tttcgaacgg
     2881 ctcgttctcc tcgccaccac gctgcagcac gtcccgcgcc gcggcaacgg tcgcaacctg
     2941 ttcgtcgagc aggctcgact cctcgaggaa gcgcaccacc tcatggccga agtccgcctg
     3001 cagtgacgat ccccagccct cgtactcttt cgagcgccgg atgtaggcct gccagcgact
     3061 cggcgacagt ttgcgtttct cgccggtcgc gtcgagcagc tcgaactcct ccagcttgtc
     3121 gcgcagcagc agctcttcca gttcggactc gcgctccagg taggtctcct ggttgccctg
     3181 tttcacgcgg tagagcggtg gcttggcgat gtagacgtag ccggcgctga tcagctccgg
     3241 catctgccgg tagaggaagg tgaggaccag ggtgcggatg tgggcgccgt cgacgtcggc
     3301 gtcggtcgcc atcacgatct tgtggtaccg ggcctcggtg atgtcgaact cttcgtggac
     3361 gccggtcccg atcgccgtga tcagcgcctg gatctcgttg ttggcgagga ccttgtcgat
     3421 ccggctcttc tcgacgttga tgatcttgcc gcgcagcggc agcaccgcct gggtgttgcg
     3481 gtcgcgcgcc tgcttcgccg agccgccggc ggagtcaccc tcgaccacga agatctcggc
     3541 caggctcggg tccttgaccg tgcagtcggc gagcttgccc ggcagggtcg agttctcgag
     3601 cgccgacttg cgggtcaggt cgcgcgcttt gcgggcggcg gcgcgggcgc gtgcggcgtc
     3661 gatcgtcttg ctgatgatcc ggcgcgcctc cggcgggttc tcctcgagga actcggccag
     3721 cttgcggttc acggtctcct cgaccaggcc tttgatcggc gggttgccga gtttggtctt
     3781 ggtctggccc tcgaactggg gatcgtggag cttgaccgag atcaccgcgg tcaggccctc
     3841 gcgcacgtcc tcgccggcga ggttgtcgtc tttctctttc agcagccctt tgttgcgcgc
     3901 gtaggcgttc agggtccggg tcagcgccgc acggaagccg gacatgtggg tgccgccctc
     3961 gtgcgtgttg atgttgttgg cgaagctgaa gatcgactcc tggtaggagt tgttccactg
     4021 catcgcgatc tcgacttcgc cgtcgtcttt gtcgccctcg aagtagatcg gctttttgtg
     4081 cagcgactct ttgttcgcgt tcaggtggcg gacgaagtcc tcgatcccgc cgtcgtactg
     4141 gaaggtgacg ctgttggtct caccgcgctc gtcgaccagc tcgatcttca ggccgcgggt
     4201 gaggaaggcg gtctcgcgca ggcgctcggc cagcgtctcg aagtcgaagc cgatctcttc
     4261 gaagatttcg aggtcgggca ggaaggtgat cgtcgtcccg tgggcgttgc tcttctcgcc
     4321 cttctcgagt ttcgtctgcg gtttgccgcg ctcgtagtcc tgcgaccaga cgaagccgtc
     4381 gcgttcgatc tgcaggtgca ggcgctcgga gagtgcgttg acgaccgagg agccgacgcc
     4441 atgcaggcca ccggagacct tgtagccgcc gccctccccg aacttgccgc cggcgtggag
     4501 gacggtgagc acgacttcgg ccgccgggcg gccctctttc tccatcgtct cgaccgggat
     4561 gccgcgaccg ttgtcggcga ccgtacagct gttgtcgggg tgcaggacta cgcggacggt
     4621 gtcgcagtgc ccggcgaggg cctcgtcgac cgagttgtcg acgatctcgt agaccagatg
     4681 gtgcagccca cgcggcccgg ttgagccgat gtacatgccg gggcgtttgc ggacggcttc
     4741 cagtccctcg aggacggtga tgtccgctgc gccgtaggag tcgctggtcg ctcctttggc
     4801 ttttttgtca ggcaatgagt cccttccgat cgacacagaa aaccccgacg cctggaggct
     4861 tgctccagcc tcgccggggc tttacgcgag ttgccataag aataccagtt cggccctgtg
     4921 gataactgtg ccgaacgtca cgttttccct gcaaatcgta gaaaaagggt tacgagacag
     4981 tgtcgctggc aaggcggaat ttgagcgctt tcggggcgga ctcgcccagg acggcccgca
     5041 gccgctccaa aagctgctcc tgcatcaggt cgagctcctg cgcccagacg ctgtcggtga
     5101 cctcgatcgt cagcgtcccg gcgcgctcgg agaccgggct ggcgaccgcc gccagctgcg
     5161 gtcctacggc ctccgcccaa gcgccctgaa ccgaggcgag gccggtctgg ggggcgacca
     5221 tcgcgcgggc ggcgcggaag gcctcggagg cctggcgggg ggctcggcgg cggctcacgc
     5281 cgcggccgcc tcggcaagct cgggcggggg catccggatc acggactcga gcgctggcgc
     5341 cggcagcgac tcttcatcgg ccgccgtgat cagcacctgg ccttcgccat cactgagtcg
     5401 cgccaccagc cgctcgcggc ggccggggtc gagctcgctc atcacgtcgt cgagcagcag
     5461 caacggcgtc acgcggcgtg ccagcagcag cgcctcgcgc tcggcgaaga gcagggcgag
     5521 cagggcggcg cgttgctggc cctgggagcc gtacttgcgc agcgagcgcc cgccggtctc
     5581 gagcttcagc tcgtcgaggt gggggcccca ggagctgcga cccatgcgga catcctgttc
     5641 gcggcgctcc tgcaatccgg cgcgaatctc ctccgccgag ccggcggcgc ggggcgcgta
     5701 ggagagcgtc gcgccgccgt cgagaccgag ctcactcgcg gcctccgcga agggttcggc
     5761 caactcggcg accgcctcct cgcgggcggc gaccagcggc gcggcggcct cggccagccc
     5821 ggcgtcccag acgtcgaggt cggcggggtt cccgtagccg gccgcgagac gcgcgagcag
     5881 cgcgttgcgc tgcgcgagcg cctgccccag ccgtttgcgc aactcggccc ggtagggcca
     5941 gcgcgcggtg acgaagccgt cgaggtgcgc tcggcgctcg gccggagggc ccttcaccag
     6001 gctgagccgg tcgggagcga agaccgccac cggcggacgg ctgcgcgcca gcgtcgccgg
     6061 gtcggtcgcg ttaccgtcga gcaggtggcg gcggccttcg ctgcggctga ccgaggcgag
     6121 caggcgccgc tcgagcccgt cctcgtcgcg gatcgtcgcc tcggcgcggg ccagcgagcc
     6181 gccgaagggg atcaggtcgc ggcggtcgga ggtgcggaac gaacggccgg tgagggcgaa
     6241 gtagagcgcc tcgaccaggt tcgtcttgcc ggtcccgttc gggccgacga cgctggttat
     6301 gccgacgccg agctcgaccc gcgcgcggtc gaggctgcgc agcgggcgcg cttcgatcgc
     6361 ggtgaccagc accggcccat cacctcagac gttgaggcgg atcggcatca ccaggtagcg
     6421 gaagtcttcg ccctcgaccg gctgcagcag ccccggccgc agcggcgaga tcaaccgcac
     6481 gaggatctcg tcgccgtcga cgctctcgat cccctccttg aggaactccg ggttgaaccc
     6541 gatctccagc gtctcgccct caaaggaggc gggcatcgtc tcggtcgcgt cgccgacgtc
     6601 gggcgtctcc gccgcgaccg tcaactcacc cgagctgaac gagagccgca gcggcgcgtt
     6661 gcgctgggcc agctgactga cgcggcgggc gacgtcgagg aaatccgagc gcggcaggcg
     6721 cacgtcgtgc tcgtaggact cggggagcag ctggcggaag ttcgggaact ggccctcgat
     6781 cagtcgcgtg gtcagcgtga tcgagccggc cgcgaacacc gcctggttcg tctgcaacca
     6841 gatctcggcc tcctccacac cctcggccga gaggatgcga cccagctcgc gcagcgcgcg
     6901 ggccgggatg ttggcctcga tctcccctcc gagctcggcc tcgagctcgg tccgtttgac
     6961 cgccagccgg taggagtcgg tggcgaccat cgtcatctct ttgccggcgg cgctgacgaa
     7021 gacgccggtg agaaccggtc gcatgtcgtc gcgcgaggcg gcgcgggcaa ccaggtcgat
     7081 gctcgcttcg agcgccttcg ccgggatctt cagcggcgac tgctcgccgg cgctcggcag
     7141 cttcgggaag tcctcggcgg ggagcgtgcg caggtggaag ctggagctac cgctgcggat
     7201 ctcgacgtcc tgctgcgcct cgcgcgactc gatttccact ttcgagtcgg caagcgagcg
     7261 cgcgacttcg gccagcaacc ggcccggcaa caggaccgtg ccctcgccct cgaccaccgc
     7321 gtcgaggtcc gtctgcagac cgaggtcgag gtcggtggcg gcgagggtga gcttctcccc
     7381 ggaggcactc agcagaaccc cggacagtgc ctgggtggcg gcgcgggtgg aaacggctcg
     7441 ggagacgatc gagagcttgg agacgagttc ttctcggttg gtcgtgagct tcaagtgagg
     7501 tgaccttctt ctgccttctt aagttggatt aggacttgtg gaaacggcgg agcgggcgag
     7561 ctatttggcc tggcgccgcg gtttccggcc tgtgggagcg gctgtgggaa aagggtgatt
     7621 cttgcactga catcggccgt caggaggggt cgcgggttgg gttgtggatt gcgccgcgta
     7681 actccgcgac cctggcggca agctcctcgt catcgccgat gccggcttcg atccggcgca
     7741 gggagttgag cacggtcgag tgatcgcgcc cgccgtagag ccggcctatc tgcggcagcg
     7801 agagatcggt catctcgcgg gtgaggaaga tggcgacctg gcgagcccgc agcggggtgg
     7861 cggcgcggct ggagccgacc agctcggcgc gcgagatgcc gaagcgctcg gcaacccgct
     7921 gctggatctc ctcggcggag gtcgcctcgg ccgagcgcgt ggacttcggg atcacctcgg
     7981 cgatcagctc ggagctgagc ggccgcgcca tcagcgaagc gtgggcgagc acgcgggtga
     8041 gcgcgccgtg cagctggcgc acgttggcgt cgatccggtg ggcgagctcg gagagggcgc
     8101 cggagtcggc gatctcgacc ccggcctcct cgaccaggcg gcgcagcacc gtgagccggg
     8161 tggtcaggtt gggcggctcc atcgccacgg tcaggcccca ctcgaagcgg tcgcgcaggc
     8221 ggctggcgag ggtcgagagt tcgctcggga tccggtcggc cgagagcacg agctggctgc
     8281 cggactcata gagagcgttg aaggtgtgga agaactcctc ctcggtggcc ggcttgccct
     8341 cgaggaactg gacgtcgtcg atcagcagca cgtcgatgtc gcgatagcgg gtcttgaagg
     8401 cctcggcgcc ggtcgacttg agcgcgatca cgaactcgtt ggtgaaggac tcggcggtcg
     8461 tgtagcggac gctgagcgcc ggcgcgttgg cctgcaggta gttggcgatc gccgccagca
     8521 ggtgggtctt gccgagtccg ggagggccgt ggaggaagag cgggttgtag gcctcggagg
     8581 gggcctcggc gacggccaga gcagcgccgt gggcgagccg gttgccgggc ccgatcacaa
     8641 agcgctcgaa ggtgtagttg gggttgagct gaacgccccc ggggcccgtg gtcgggtcgc
     8701 tgccgccgcg gggcgaggag aagctgacat tgcagagcgg cgtcccggcg tcgaccagcg
     8761 cttcggcgat cagcgaggag tagcggcgct cggcccaggc ccggacccgc tcctcgcgag
     8821 cggtcaggta gagggtgtcg tcagtggccc cggcaacctg gagcggctcg aaccagatcc
     8881 ggtaggtcga ctcgggaacc gacgcccgta gtcgctcttg ggtcgcccgc caaagggcgt
     8941 cgagatcttg gcttgagggg tcattgggca ctcggtcggc gaccatagcg gcgtgcctga
     9001 tcaggcatgg tccatgggtc gcaaatcccg tagaaaagat ttcggggccg cgtcttcgct
     9061 ctccgtccgt atacttctcg acccgatgaa gcggacctat cagccgaaga agcggaagcg
     9121 cgccaaaacc cacggcttcc gggccaggat gagcacgcgc ggaggccgcg cgatccttaa
     9181 gcgccgccgg gccaaagggc ggaagcgcct tactccttag agatggcgtc gaagcgacgg
     9241 cgcctgtcgc gcagcggcga gttcgaccgc gtatatcgcg acggttcatc ccatgcgacc
     9301 cgctacctcg tgctctacac gtttccccgc cgcgacgaaa gtggcgatga cgtgaggctc
     9361 ggagtgtcgg tcagccgcaa ggtcggcggt gcggttgagc gcaacaaggc gaagcgggcg
     9421 ctgcgggaag cgttctggag cctttctgat cgtctgccgg aagggcacga cttcgtcgtc
     9481 gttgcccgcg gtgagatcaa cgaactggtc gaccgcgacg gcaccgcagg cgtggccaag
     9541 agcctggaag aggctctggc ggcggccacg ccgggaaagc gttccacgtg aaacgcgtcg
     9601 cgctcgcccc gatccgggcc taccaacgct ggatctcacc ctcgcgaccc cgtcgctgcc
     9661 gctacgagcc gacctgctcc gcctacgcgg tcgaggccgt cgagcgcttc ggggttgtgc
     9721 gcgggatgat cctcgccgcc tggcgcctgc tgcgctgcaa ccccttcaac cacggtggat
     9781 ttgacccggt gccgaagcgc ttcactctgc gcggcctcgg gcacgtgcac ccggccgagt
     9841 accacggcga ggcgcaggtc tgatgagcct cccgttcgga ttccagctcg cgccgcagtt
     9901 cctggtcgac atcgccgacg aagtcctcgt cttcttccac gactccggcc tctcctgggg
     9961 catgtcgatc gtcgccctga cggtctgcat gcgaaccctg ctgatcccgc tgacctacaa
    10021 acagctcaaa ggcatgcggg cgatgcaggc tctgcagccg cagatcaagg aactgcagga
    10081 gaaatacaag aacgacaaac agcgcatgca gcaggaaatg atgcgcttct acaaagagaa
    10141 caaagtcaat ccctttgcct cttgcatccc cctgctggcg cagctgccgg tcttcatcac
    10201 gctcttctac gtcctccagc acgacctccg cttcgacatc tgcggacaga cggcggaaac
    10261 ctgcggcgag ttcgcggtca cccagggcca ctccgccggc ttcttcggcg aaagcttctt
    10321 cttcatcaac gacctgacct cgaaggcgac gggcagcgaa ctgatcgtgc tgctggtgat
    10381 ctacgtcggg acccagctga tctccggcat ggtgatggcg gtcaccgcgg acaaaagcca
    10441 gcgcatgctg atgttcctgc tgccgctctt cttcgtcccc ttcatcctca acttccccgc
    10501 cggcctgatc ctctactgga tcacgaccaa cttctggacc atcggccagt cgttcgtggt
    10561 caaaaagctg atcccagtgc cggttgcggc gaccccagcg ggcgcaacgg caatcaaaga
    10621 ggcaaaaccg ccccccaaac cgccgaaaaa aaagaaacgc aagcgtcgct aagggcctcc
    10681 cgaaccgggg gccctttttg ttgggactag cccttggggc gcttcttggc cattcctggc
    10741 cgccgaggga gattggcggg ggttgggccg gacttggtga ggatgtggag gtggcggtgc
    10801 tcggagccgg cgcggtggcc gacgtcgagg atctgcccag gcgccatcgc cagggactcc
    10861 gccgcccgct cgagctgcgc ctcctcctcg gcgtcgcgtt tgcccttcca ggcgaccagg
    10921 tggccgcctt cctgaagcag cgggctggcc agctccgcga gggtcgagag gcgggcgacg
    10981 gcgcgggcgg tgacggcgtc gtaggcctcg tttccttccc cagcggccca atcctcggag
    11041 cgcaggttga gcacagaggc gttggcgatc cccgccgccc cgatcgcccg ctgcatgaac
    11101 tcgcacttgc gcgagaccga ctcgatcagg tcgacccggg cgccgggcag ggcgacggca
    11161 agtacgaggc ccggaaaacc cgctcccgcc ccgacatccg cgatccgctt cgcttcgcgc
    11221 agctcaggca cctcgagccc ggtgaggctg tcctcgacat ggaccttcca ggcacgcttg
    11281 tcgactaccg agctgaccga ggctcgctcc tcggcgagca gctccagcac ggtctccagc
    11341 gccctgcggc cagcctcgtc cagcgccggg gggccgtcgc tcactcggag acgagcggcg
    11401 ctacgacgac gcagcggtgg ggctcgtcgc cctcgctgta ggtctcgacc ccggcccggt
    11461 ccttcagccg ctcgtgcacc acacggcgct cctgggcgct catcgggtcc atctcgacga
    11521 cccggttgtt gctcagcgcc tgctcggcgg cgcggtcggc gcgcccctcg agcgtctcgc
    11581 ggcgccgctc ccggtagccg gcggcgtcga cgatcacccg tttgcgctcg cgcagaccgc
    11641 ggaaagccgc ctggtaacag agcagctgca gcgcgtcgat cgtctggccg cgcttgccga
    11701 tcagcaggcc gtagtcggcg tcgccctcga ccgtgatcga gatcacgtcg tcgtcctcct
    11761 cgacctcaac ctcgccgtcg aggtcaagct cgtcgagcac gccctcgacc agctcgcggg
    11821 cgcggtcggc gggtgcatct ggccattcga aggtctcgga cacggcccgc aggctatccg
    11881 accgctcgcc gacgcgtgca gataattagg ctgcctgcga tgggcaaggt ctacgcggtg
    11941 gcgaaccaga agggcggagt cggtaagacg accagcgccg tcaacctcgc ctcctgcgct
    12001 gcggccgagg gtcagcagac cctgctggtc gatctcgacc cccaaggcaa cgcgaccacg
    12061 gcgctcggcg gtgagaagga gatgcacccc tcttcctacg actgcctgac cggcgacacc
    12121 tcggttgccg aggcggcccg tcccgctggc cccgacaacc tctggctggt ccccgccagc
    12181 cgcgacctcg ccggcgccgc ggtcgagctg ccccggatcg agggctcgga gacgcgcctg
    12241 cgcgaacggc tcggcccggt ccgcgagcgc ttcgccgtca ccctgctcga ctgcccgccc
    12301 tcgctcggcc cggtcagcgt caacgcgctg accgccgccg atcgcgtgat cgtgccggtg
    12361 caggccgagt atctggcgct ggagggcctg gttcaattcc tcgagacgct ggggttgatc
    12421 aggcagcagc tcaaccctca gctggaggtt gcgggtttgc tgatcaccat gtacgacgat
    12481 cgcaccaggc tggcaattga cgtcgagtcc gagctccgcc gccacttcgg cgagatggtc
    12541 ttcgagaccg tgatcccgcg cagcgtccgt gtcgccgagt cccccagtta tggtctgccg
    12601 gtgaccgagc acgcccccgg atcgcgcggc gccgtcgcct accaggctct ctcgaaggag
    12661 ttgatcagcc gtggctagcg gtaccaaacg cgggatcggc cgcggcctcg cggcgatttt
    12721 gccggaagtc gagaccagct ccgccggtga gctgcgcgag ctcccggtcg ggctgatcaa
    12781 gccgaacccg aaccagccgc ggacgaagtt cgaccccgag gccctggcgg ctctggccgc
    12841 ttcgatcgag gcgaccggcg tggtccagcc gctgctggtc cgtcccctcc ccgacggcag
    12901 cttcgagctg gtcgccgggg agcgccgctg gcgcgcggcg cagcaggcga acctcgacaa
    12961 agtccccgcg gtgatccgcg accaggagga agcggagcgg ttgcaggcgg cgctgatcga
    13021 gaacatggtc cgggaggacc tcaacccggt cgaggaggcg aaggcctgcg ccgccctgat
    13081 cgaggacctc ggcctgacca aagaggagct ggcgaaacgg gtcggccgct cccgccccgc
    13141 cgtctccaac ctgatccggc tgctcgagct gcccgacgag ttgctcg
//
LOCUS       DASRIG010000142         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3725015, whole genome shotgun sequence.
ACCESSION   DASRIG010000142 DASRIG010000000
VERSION     DASRIG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725015"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFB52_03925"
     CDS             complement(<1..1350)
                     /locus_tag="VFB52_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZO05510.1"
                     /translation="MSGLPVMSEVSLNDKYVADSGTVLISGVQALVRLVLEQRRLDEA
                     RGLDTAAFVSGYQGSPLGGFDRELQRAGKYLDALGVVFQPGLNEELGATAVAGTQLIG
                     ELELRRHEGIVGFWYGKNPGLDRAADAIRHGNVSGTAPLGGAVALVGDDPSCKSSTIP
                     SSCEPICRSLIMPVLAPGTVRELLDFGLHAVALSRASGLWVAVKVVADIADASATVEL
                     DGIADRIPVPETRPFKPPILLAASSLDAERDQLGSRLDRALAYIRAAGLNEIVFEPSR
                     PRLTVVAAGVAHEAVVRALADLGVGDAELERIGLRLAKVAMPWPLDPAFVAELAAGVE
                     EVLVIEDKLPFLESQIKEMLYGGPQTPRVVGGGAAVGEAVLSRGGGVSAEEVAAALAA
                     RLPGGALPGPAAAAPVEFADGSAVELRQLPARTPYFCSGCPHNRSTKAPDDSLVGVGI
                     "
     gene            complement(1347..2480)
                     /locus_tag="VFB52_03930"
     CDS             complement(1347..2480)
                     /locus_tag="VFB52_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05511.1"
                     /translation="MSTYLLSDEQREVRELARRFAEAEVAPGAAERDEAHRFPSDLLD
                     HLGILGLLGVPVAEELGGGGADFLSYCLVLEELSRADASLGVTLAVHTGAVTLPLLAH
                     GSAEQVERLVPPLAAGGRIGAFALTESAGGSDPKAMRSQVDAELRLSGTKQWVTNGSH
                     AGTVLAFAREGDAISAFVADGADPGLAVLREERKMGLHSSSTADLAFERVRVERLGEA
                     GAGLRIALAALDGGRIGIAAQAVGIAQAGLDVAVAYARERYAFGRPIAGFEAIQQKLA
                     EMETEIEAARALTWRAARLKQANRPHGAEGAQAKLFASRVARHWTGEAIQVLGGYGYT
                     KEFPVERYYRDAKVTEIYEGTSEIQRLVIARRLLGDVAIGGRG"
     gene            complement(2477..>2996)
                     /locus_tag="VFB52_03935"
     CDS             complement(2477..>2996)
                     /locus_tag="VFB52_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HZO05512.1"
                     /translation="HVGSELTRLLCDAGATVEVADIDRGRSEAVAAAYGATVVDPAEA
                     AVRDCDVFAPCAMARVIDERTVPRLRCRVVAGAANDTLADDGLVAEVAARGILYVPDF
                     LANAGGVIHIHARREGFSESQLRAAVLEIGDRVAAVIDRATHESTLPLAVARADAREV
                     LEGGLHPIARVG"
BASE COUNT          452 a         1064 c         1101 g          379 t
ORIGIN      
        1 gatcccaacg cccacgaggc tgtcgtcagg cgccttggtc gagcggttgt gaggacagcc
       61 ggaacagaag tagggcgtcc gcgcgggcag ctgccgcagc tccactgcgc tgccgtcggc
      121 gaactcgacc ggggcggccg cggcgggccc gggcagcgcg ccgccgggca gccgcgcggc
      181 gagcgcggcg gcaacctcct cagcactgac tccaccgccg cgcgacagca ccgcctcgcc
      241 gaccgcggcg ccgccgccga ccacccgcgg tgtctgcggg ccgccgtaga gcatctcttt
      301 gatctgactc tcgagaaagg gcagcttgtc ctcgatcacc agcacctcct cgaccccggc
      361 ggcaagctcg gcgacgaagg caggatcgag cggccagggc atcgcgacct tcgccagcct
      421 tagtccgatc cgctcgagct cggcatcgcc gacgccgagg tcagcgaggg cccggacgac
      481 ggcctcgtgg gcgacgcccg cggcgaccac ggtgagccgg ggccggctgg gctcgaagac
      541 gatctcgttg aggcccgcgg cgcggatgta ggcgagggcg cgatcgagcc gcgagccgag
      601 ctgatcgcgc tcggcgtcaa gcgagctggc ggcgagcagg atcggcggct tgaacgggcg
      661 cgtctcaggc acagggatgc ggtcggcgat cccgtcgagc tcgaccgtgg ccgaggcgtc
      721 ggcgatgtca gcgacgacct tgaccgcaac ccagaggccc gaggctcgcg agagcgcgac
      781 cgcatgcagg ccgaagtcga gcagctcgcg cacggtgccc ggcgcgagca ccggcatgat
      841 caggcttcgg cagatcggct cacaggagct cgggatcgtc gaggacttgc aggaggggtc
      901 gtcgccgacc agcgcaacgg caccacccag cggcgcggtg ccgctgacat tgccgtggcg
      961 gatcgcgtcg gctgcgcggt cgaggccggg gttcttgccg taccagaagc cgacgattcc
     1021 ctcgtggcgg cggagctcga gctcgccgat cagctgggtg ccggcgaccg cggtcgcgcc
     1081 cagctcctcg ttgaggcccg gttggaagac gacgccgagc gcgtcgagat acttgcccgc
     1141 gcgctgcagc tcgcggtcga agccgcccag cggcgatccc tggtagccgc tgacgaaggc
     1201 ggcggtgtcg agcccacggg cctcgtcgag gcggcgctgc tcgagaacga gccggaccag
     1261 cgcctgcaca ccgctgatca gcaccgtgcc ggagtcggcg acgtacttgt cgttgagcga
     1321 gacctctgac atgacgggca gcccgctcat ccccgtcccc cgatggcaac atcgccgagc
     1381 aggcggcgcg cgatcacgag ccgctggatc tcgctggtgc cctcgtagat ctcggtcacc
     1441 ttggcatcgc ggtagtagcg ctcgaccgga aactccttcg tatatccgta gccgcccaag
     1501 acctggatcg cctcgccggt ccagtggcgg gcaacacgcg aggcaaagag cttcgcctgc
     1561 gctccctctg cgccgtgagg acgattggcc tgcttgagcc gggcggctct ccaggtcagc
     1621 gcccgggcgg cctcgatctc cgtctccatc tcggcgagct tctgctggat cgcctcgaaa
     1681 ccggcgatcg gacggccgaa ggcgtagcgc tctcgggcgt aggcaacggc gacgtcgagg
     1741 ccggcctggg cgatgccgac cgcctgggcg gcgatcccta tccggccgcc atcgagggcc
     1801 gcgagcgcga tccgaagccc ggccccggcc tcgcccagcc gctcgacgcg gacgcgctcg
     1861 aaagcgaggt cggcggtcga agaggaatgc agacccatct tgcgctcctc gcgcaggacc
     1921 gcgaggccgg ggtcggcgcc gtcggcgacg aaggccgaga tcgcgtcgcc ctcgcgggcg
     1981 aaggcgagca ccgtcccggc gtgcgagccg ttggtgaccc actgcttggt gccgctcaga
     2041 cggagctcgg cgtcgacctg gctgcgcatc gccttcgggt ccgagccgcc ggccgactcg
     2101 gtcagggcga aggcgccgat ccgcccgccg gcggcgagcg gaggcaccag ccgctcgacc
     2161 tgctcggcgc tgccgtgggc gaggagcggc agcgtcacgg cgcccgtatg aaccgcgagc
     2221 gtgacgccaa gcgaggcgtc cgcccgggag agctcctcga ggaccaagca gtaagagagg
     2281 aagtctgcac cgccgccgcc tagctcctcg gccaccggga cgccgagtaa cccgagaatg
     2341 ccgaggtggt cgagcaggtc gctggggaag cggtgggcct cgtcgcgctc ggcggcgcca
     2401 ggcgcgacct cagcctcggc gaaacggcga gcgagctcgc ggacctcgcg ctgctcgtcg
     2461 ctgagcaggt aggtactcat ccgacccgag cgatggggtg aagaccgccc tcgaggacct
     2521 cgcgggcgtc agcccgcgcc accgccagcg gcagcgtcga ctcgtgggtg gcgcggtcga
     2581 tgacggcggc aacgcggtcg ccgatctcta gcacggcggc gcgcaactgc gactccgaga
     2641 acccttcgcg ccgggcgtgg atgtggatca cgcccccggc gttggcgagg aagtcgggca
     2701 cgtagaggat gccgcgggcc gcgacctcgg cgacgagacc gtcgtcggcg agggtgtcgt
     2761 tggcagcgcc ggcgacgacc cggcagcgca gccgcgggac tgtccgctcg tcgatcaccc
     2821 gggccatcgc gcatggcgcg aagacgtcgc agtcgcgcac ggcggcctcg gcgggatcga
     2881 cgacggtcgc gccgtaagcg gcggcgaccg cctcggaccg gccgcggtcg atatcggcga
     2941 cctccaccgt ggcccccgca tcgcacagca gccgggtcag ctcggagccg acgtga
//
LOCUS       DASRIG010000143         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2683566, whole genome shotgun sequence.
ACCESSION   DASRIG010000143 DASRIG010000000
VERSION     DASRIG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683566"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFB52_03940"
     CDS             complement(<1..804)
                     /locus_tag="VFB52_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05513.1"
                     /translation="MGNRSPRGFAVIAVVVCLALLSAGPATAGDPNHRPPDIELTAAG
                     NEGFINARAKWLAYFDTGLALDPKAEYRRDSKVTFTCRIDGRPVKCNAGFQPVDKVEV
                     EYGRPNKWAQTGFFGGAIQAPDDLAPGPHTITVRARDEDGPEAAPKSVEITFDPTPPS
                     APTLTEKPLGRSWDHKPDFVYSSSDDMRLVRPEGEGMFIASLRRLKPTYQRYREDSGE
                     SSMVSWLPECPDLLTCSIESRVVYWSEEDWYSFAEDEWLLPGLYEFRARA"
     gene            complement(829..1182)
                     /locus_tag="VFB52_03945"
     CDS             complement(829..1182)
                     /locus_tag="VFB52_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05514.1"
                     /translation="MITKLDFVGIPSTDADRSRQFYVETLGLKPDPKSDYEFWVGETC
                     FGIWEPAKQGMEFAPQKNAHPALHVDDVAAARTELEAKGVQFNGDTFDTGVCHMALFC
                     DPDGNDLMLHSRYAD"
     gene            complement(1261..>2003)
                     /locus_tag="VFB52_03950"
     CDS             complement(1261..>2003)
                     /locus_tag="VFB52_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05515.1"
                     /translation="LTAAGPAIVHFIDYAQLNSVRTLPYLSEWARRYREQGLSVIGVQ
                     APRFPFGADPEAVTAGLAALGVEFPVVIDTERELWAAYGCEGWPSLFLWSLGGALAWA
                     HFGEGEYWGTEGAIQEELREIDALRELPEPMAPLRPTDGPGARVMAPTPEIFPSESWE
                     RAWTAGEDGEVLELDYQAGGVYVTVEGEGQVEAEIDGDWGEPLAAGPHPSLLTVAEHP
                     CHEAHSLVLRPSPGLRVWSISFAAGVPG"
BASE COUNT          279 a          674 c          709 g          341 t
ORIGIN      
        1 ggcccgggcg cggaattcgt agagacctgg gagcagccac tcgtcttcgg cgaagctgta
       61 ccagtcttct tcggaccagt agaccacgcg tgattcgatc gagcaggtga gcaggtccgg
      121 acattccggc agccacgaca ccatcgagct ttcgccggag tcttcgcggt agcgctggta
      181 ggtcggcttc agccgccgca gcgaggcgat gaacatgcct tcgccttcgg ggcgcaccag
      241 gcgcatgtcg tcgctcgagc tgtagacgaa atccggcttg tggtcccaac tgcggcccag
      301 cggcttctcg gtcagcgtcg gcgccgacgg gggcgttggg tcgaaggtga tctcgacgct
      361 cttcggcgcc gcttcgggcc cgtcctcgtc gcgggcgcgg accgtgatcg tgtgcgggcc
      421 tggggcgagg tcgtcagggg cttggatcgc cccgccgaag aagccggtct gcgcccattt
      481 gttcggccgg ccgtactcca cttctacctt gtcgacgggc tgaaagccgg cgttgcactt
      541 aaccggccgg ccgtctatcc ggcaggtgaa ggtcaccttg ctgtcacgcc ggtattccgc
      601 cttcgggtcg agcgccaggc cggtgtcgaa ataggcgagc cacttggcgc gcgcgttgat
      661 gaacccttcg ttgccggccg ccgtcagttc gatgtcgggc ggccgatggt tggggtcgcc
      721 cgccgtcgcc ggcccggcgc tgagcagcgc cagacagacg acaacggcga ttacggcgaa
      781 tcctcgcggc gagcggttcc ccatcggggg tgaggataga acccggggct agtccgcgta
      841 tctgctgtgc agcatcaggt cgttgccgtc cgggtcgcag aacagcgcca tgtggcagac
      901 gccggtgtcg aaggtgtcgc cgttgaactg gacccccttc gcctccagct ccgtccgcgc
      961 cgcggccacg tcgtcgacgt gcagcgccgg gtgggcgttt ttctggggcg cgaactccat
     1021 cccctgtttg gccggttccc agattccgaa gcaggtctcg ccgacccaga actcgtagtc
     1081 gctcttcggg tccggtttga gcccgagcgt ctcgacgtag aactggcgcg agcggtcggc
     1141 gtcggtggac gggatgccga cgaagtcgag cttggtgatc atcgttcctc ctggtgattc
     1201 cgtggttggg aggggggtcc ccagccaacc tacccgggaa cgcgatgtct atccaggcgc
     1261 ttacccaggg acgccggcgg cgaagctgat cgaccagacg cgcaggccgg gggaggggcg
     1321 caacacgagg ctgtgggctt cgtggcaggg gtgctcggcg accgtcagca gactggggtg
     1381 agggccggcg gcgagcggct cgccccagtc gccgtcgatc tcggcctcga cctgtccctc
     1441 gccctcgacg gtgacgtaga cgccgcccgc ctggtagtcg agctccagca cctcgccgtc
     1501 ctcgccggcg gtccaggcgc gctcccatga ttcgctcggg aagatctccg gggtcggcgc
     1561 catcacccgg gcgccggggc cgtcggtcgg gcgcagcggc gccattggct cgggcagctc
     1621 gcgcagggcg tcgatctcgc gcagctcctc ctggatcgcc ccctccgtgc cccaatattc
     1681 accttcgccg aagtgcgccc aggcgagcgc gccgccgagg ctccagagga agaggctggg
     1741 ccatccttcg cagccgtagg cggcccagag ctcgcgctcg gtgtcgatca cgactgggaa
     1801 ctcgaccccg agcgccgcca gcccggcggt gaccgcctcc gggtcggcgc cgaaggggaa
     1861 gcgcggcgcc tgcacgccga tcacgctgag cccctgctcg cggtagcggc gcgcccactc
     1921 gctcaggtaa ggcagcgtgc gcacgctgtt cagctgcgcg tagtcgatga agtggacgat
     1981 cgccgggccg gccgcggtta ggc
//
LOCUS       DASRIG010000144         8788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3727314, whole genome shotgun sequence.
ACCESSION   DASRIG010000144 DASRIG010000000
VERSION     DASRIG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727314"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFB52_03955"
     CDS             <1..310
                     /locus_tag="VFB52_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HZO05516.1"
                     /translation="PQTPGVAIGVREDHWCCEYCEGELGDSAEDWREQAVSVETSISE
                     RLAEADMYVRQRQGAPPVLLAEHFCPHCAGLLLADVYPEGFEGLRVPQLKKSGAALGV
                     "
     gene            313..1881
                     /locus_tag="VFB52_03960"
     CDS             313..1881
                     /locus_tag="VFB52_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05517.1"
                     /translation="MSAGAAGGPRVGYSVADLIRKGGSGGRPDAEALFFEGRRVSFGE
                     LDSRSDRVAAGLRARGFAKGERAAILAHNSVEFVETYFALAKLGGVVVPVNYLLRAAE
                     VDYALRDSGATWLFADGSGLDVVAEILGDHPELNVVGLEAGASGAIPYEDLLRCADPA
                     PEAGEVQPEDLLLLQYTSGTTGFPKGAMHTHSTVLFNALHQIADFGLSADDVYVLQPA
                     LCWAAGINNIALALWSLGGRVVMRPSRGFDAEELAGAIATEGGTVLIGVASVLRLLVE
                     SGTFTRHDLSRLRLIAVGGEAVAPALLEKAREQLPQTEIGQVYGQTEFPTLMAFLGAE
                     RALEKPASTGRATSLCELKVVDPEGAELPPGEHGEIICRSPASMKGYWNKPEQTAETI
                     VNGWLRTGDRGYTDEEGFLYIAGRSKEMYISGGLNVYPAEAEVVLAEHPEVAEVAVKG
                     IADERFGEVGCAYLVPAGEGLDLAAVEAFCRERLASYKVPKRWVVRDQPLPRTASGKA
                     EKHRLELPLETAGP"
     gene            1878..2894
                     /locus_tag="VFB52_03965"
     CDS             1878..2894
                     /locus_tag="VFB52_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO05518.1"
                     /translation="MKAGVWSGGAEISCREVADPVPGPGELLVEIAYCGICGTDLHIL
                     DGEFDVGPPPQVLGHEASGTVVAAGPGADPALVGEPVGLNPIGPCGICAWCRRGLVTH
                     CRRPNYSASGFAELGLYRSHQVHPLPAGVALRDAALLEPWATALRAVEAARLEPGENV
                     LVIGAGSLGLLVATAARLCAAATVTVSEPRSRNRRLALDRGADAAVDAGRDDLAALAA
                     EVGELGGFDAVFEVAGAGPALAAAPSLLRRAGRLVVLGVHAPAATVPFEPRALTDREL
                     SVLGSFGGGSSFARAAALLGRIDAASLVTAEAPLEEIGALFERVRGGDEVKAMVRASG
                     EGRG"
     gene            complement(3737..4966)
                     /locus_tag="VFB52_03970"
     CDS             complement(3737..4966)
                     /locus_tag="VFB52_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZO05519.1"
                     /translation="MSAATAGGPLAGLRVVEGSAFVAAPLGGMTLAQLGAEVIRFDQI
                     GGGLDHGRWPLAADGQSLFWAGLNKGKRSIALDLRSPEGRELASALITAPGEEAGLFL
                     TNFPARGWLGYEALRARREDLIMVALTGNPDGTSEVDYTVNPATGFPWATGPRNLAEP
                     LNSVLPAWDVAMGTLAAVGLLAAERRRGRGGAGELISLSLSDVAFAMVGNLGRIAEAE
                     LGARDHAKDGNYLYGAFGHDFETGDGRRAMVVALTARQWAALVEATGIEEACRGIEAA
                     TGHDLSTESGRFEARDLIAAILRPWFAGRDLATVREIFHGTGVSWGPYQTFRQLVDED
                     PRVSTENPMFEAVEHPGVGTYLMPGSPLRFRDAGSLPVQRAPQLGEHTEQVLAEVLGL
                     DGTEIARLHDAGTVASP"
     gene            complement(4963..6159)
                     /locus_tag="VFB52_03975"
     CDS             complement(4963..6159)
                     /locus_tag="VFB52_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZO05520.1"
                     /translation="MSNRTFVIGVGMTKFDKPGSKDGDYPDWVSEAGSKALEDAGVSF
                     EQVQRAAAGYCYGDSTAGQRAIYSLGLTGIPIVNVNNNCSTGSSALWMAREAVRGGLA
                     ECALAVGFEKMQRGSLAVGFADRADPIGRHKERMAELRAPEDDPFAPEMFGNAGRDHM
                     QRYGSTPDHFAWIGWKNHKHSVNNPYAQFQDEYTLEEIQASRMIHEPLTKLQCSPTSD
                     GAAAAIVAGERFVEQHGLWDRAVEIAGQAIVTDLSNTFAVDTDCMRLVGADMSREAAR
                     QAYEEAAVAPSEVDVCELHDCFSTNELISYEALGFAAEGEGQKLVEAEATTYGGSGPV
                     VNPSGGLISKGHPLGATGLAQCSELVWQLRGESGERQVDGAEVALQHNIGLGGAAVVT
                     VYRPAS"
     gene            6364..7512
                     /locus_tag="VFB52_03980"
     CDS             6364..7512
                     /locus_tag="VFB52_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO05521.1"
                     /translation="MLALTALLAIGVAACGGGDDSTGGGGDTSGGSAEADASVKDAEA
                     YLAKYGKGVGGPLPTEPAKPQPGKDVWILSCAQAASGCASITAAIKEAAEEVGWKATV
                     VDGAGDVSKWNSAVDQARVAGADALFSVSVDCAPIKQTLERAKAAGMITEGVSAYDCS
                     DPISGGGEPLFTHMFEGPIKGPESWAKLAEAGGGLQAAWTVVHVPGEKEAIVFTSQDV
                     AVTQYINKGFKDQFNCDECSIASEVPFHLTELGSPLTQKAQTAMLKYPDANVLAPPYD
                     PVSESTAQAIVNAGKVGTVYNTGLLATPSNVELIRSGRGQEMTVAWDSRWFGWAAVDD
                     VIRLFNDQEPVYSGWDQGIVDKENLPEGEVYEAPVDYIANYEKLWGLD"
     gene            7570..>8788
                     /locus_tag="VFB52_03985"
     CDS             7570..>8788
                     /locus_tag="VFB52_03985"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO05522.1"
                     /translation="MGDVEKFFGATHALRGVSLELNRFEVHGLVGENGCGKSTLVKVL
                     SGVHHADAGWVEAGEKKISAEDVTPADAREMGVTVVHQEAPIFPSMTIEDNLSLGRRY
                     PTRMGRVDRGAIRKQAEEVIEHYEIPAGPRTPAHELNPAVRAMVAIARALRDSASRDS
                     GTLILDEPTAGLNEVEADDLLEAVRRYAARGQAIMLVSHRLDEIVGACQRVTVMRDGV
                     VVTTLHGAEIDRGNIVRQMVGSNLAKEYEADAARVAEASANPVVLQFDGVSTDQLQDV
                     SLAAHEGEILGLAGQLGAGCSEALQIAYGIAPYKGGKVTLAGRRLPSRRAGRAQSDLV
                     GYVPADRPGEGMFGGRSVRENLGVAENRRYWRGGFFRRGAESRDSEALIEQFRIKPDD
                     PGALMTNLSGGNQQ"
BASE COUNT         1298 a         3024 c         3174 g         1292 t
ORIGIN      
        1 gccgcagacg cccggcgtgg cgatcggcgt ccgcgaggac cactggtgct gcgagtactg
       61 cgagggcgag ctcggtgaca gcgccgagga ctggcgcgag caggcggtca gcgtcgagac
      121 ctcgatctcc gagcgcctcg ccgaggccga catgtacgtg cgccagcgtc agggcgcgcc
      181 gcctgtcctg ctcgccgagc atttctgccc gcactgcgcc ggcctgctgc tcgctgacgt
      241 ttacccggag ggcttcgagg gcctgcgcgt tccgcagctg aagaagtccg gggcggcgct
      301 cggggtctga gcgtgagcgc aggcgctgcg ggtggtccgc gggtcgggta ctcggtcgca
      361 gacctgatcc gcaagggcgg gtcgggcggc cgccccgatg ccgaggccct cttcttcgag
      421 ggccggcggg tcagcttcgg cgaactcgac tcgcgcagcg accgggtcgc cgccggactg
      481 cgcgcgcggg gcttcgcgaa gggcgagcga gcggcgatcc tcgcccacaa ctcggtcgag
      541 ttcgtcgaga cctacttcgc gctcgccaag ctcggcggcg tggttgtgcc ggtcaactat
      601 ctgctgcgcg cggcggaggt cgactacgcc ctgcgcgaca gcggcgccac ctggctcttc
      661 gccgacggga gcgggctcga cgtggtcgcc gagattctcg gcgaccaccc ggagctgaac
      721 gtggtcgggc tcgaggccgg cgcgtccggc gcgatcccct acgaggacct gctgcgctgc
      781 gccgacccgg cccccgaagc cggcgaggtg cagcccgagg acctgctgct gctccagtac
      841 acctcgggga ccaccggctt cccgaagggc gcgatgcata cgcactcgac cgtgctgttc
      901 aacgcgctgc accagatcgc cgacttcggg ctctccgccg acgacgtcta cgtcctgcag
      961 ccggcgctct gctgggcggc cgggatcaac aacatcgcgc tggcgctgtg gtcgctgggc
     1021 ggccgcgtgg tgatgcggcc gagccgcggc ttcgacgccg aggagctggc cggcgcgatc
     1081 gccaccgagg gcggcacggt gctgatcggc gtcgcctcgg tgctgcggtt gctggtcgag
     1141 tccggcacct tcaccaggca cgacctctcg cgcctgcgtc tgatcgccgt cggcggcgag
     1201 gcggtggctc ccgcgctgct ggagaaggcg cgcgagcagc tgccgcagac cgagatcggt
     1261 caggtctacg ggcagaccga gttcccgacc ctaatggcct tcctcggtgc cgagcgggcg
     1321 ctggaaaagc ccgcctcgac cggccgtgca acctcgctct gcgaactcaa ggtggtcgac
     1381 ccggagggtg ccgagctgcc acccggagag cacggtgaga tcatctgccg ctcgcccgcg
     1441 tcgatgaagg ggtactggaa caagcccgag cagaccgccg agacgatcgt caacggctgg
     1501 ctgcggaccg gcgaccgcgg ctacaccgac gaggagggct tcctctacat cgccggccgc
     1561 agcaaggaga tgtacatcag cggcgggctc aacgtctatc ccgccgaggc cgaggtggtg
     1621 ctcgccgagc accccgaagt cgccgaggtc gcggtcaagg gcatcgccga cgagcgcttc
     1681 ggggaggtcg gctgcgccta cctcgtgccc gccggggaag ggctcgatct ggccgcggtg
     1741 gaagcgttct gccgcgagcg cctggccagc tacaaagtcc cgaagcgctg ggtagtgcgc
     1801 gaccagccgc tgccgcgaac ggcctctggc aaggcggaaa agcaccgtct cgagctgccg
     1861 ctggagacag caggaccgtg aaggccggcg tctggtcggg cggcgcggag atctcctgcc
     1921 gcgaggttgc cgacccggtc cccggccccg gcgagctgct tgtcgagatc gcctactgcg
     1981 gcatctgcgg caccgacctg cacatcctcg acggcgagtt cgacgtcgga cctcccccgc
     2041 aggtcctggg gcacgaggcg tcgggcaccg tggtcgcggc cggtccgggc gccgacccgg
     2101 cgctggtcgg cgagcccgtc ggcctcaacc cgatcggccc ctgcgggatc tgtgcctggt
     2161 gccggcgcgg gctcgtcacc cactgccgcc ggcccaacta cagcgccagc ggcttcgccg
     2221 agctcgggct ctaccgttcg caccaggtgc acccgctgcc cgccggcgtg gcgctgcgcg
     2281 acgccgccct gctcgagccc tgggcgaccg cgctgcgcgc ggtcgaggcg gcgcggttgg
     2341 agccggggga gaacgtgctg gtgatcggtg cgggctcgct cgggcttttg gtcgcgaccg
     2401 cggcacgact gtgcgccgcc gccacggtga ccgtctcgga gccgcgctcg cgcaaccggc
     2461 gcctggccct cgaccgcggc gccgacgcgg ccgtcgacgc gggccgcgac gaccttgccg
     2521 cgctggccgc cgaggtcggc gagctgggtg gcttcgatgc ggtcttcgaa gtcgccggtg
     2581 ccggcccggc gctggccgcg gcgccgtccc tgctgcgccg cgccggccgc ctggtcgtgc
     2641 tcggcgtgca cgcgcccgcg gcaaccgtcc cgttcgagcc gcgggcgctg accgaccgcg
     2701 agctgagcgt gctcggctcc ttcggcggcg gcagcagctt cgcccgcgcc gcggccctgc
     2761 tggggcggat cgacgcggcg tcgctggtca ccgcggaagc gccgctggag gagattgggg
     2821 cgttgttcga gcgggtgcgc ggaggcgacg aggtgaaggc gatggtgcgg gcgagtggcg
     2881 agggacgcgg ttgagcggag gatttttttg acgtaggccg gttcgtattt gacctgccag
     2941 tgggagaagc gcaggcgggt ataaccagcg gcggtgtggg cctggtcgcg cttcaggtca
     3001 cgggcctgag atgaaggcgt gcggtggtag cgcaggccgt cggtctcgac gaccaggcga
     3061 aactcgggcc agaagaaatc gacctcgtag ccgttcaccc agactttggt ctcagggagg
     3121 gagagtccga ttcccagggt caaggggagg aaaagccgtt ccagctcctc gtccgagagc
     3181 acgaaggtgt cccggtcgag caaacgccca agcaccttga cgcccggttg accggagtag
     3241 gcgtcgagtg actcccgcaa gtcgtccgcc aggatcactt ccagcttgtc ggcctcgttg
     3301 atcgagcgca gcaggtactt cggtccctgc acggtggcga ggtcgatcag ggtgcggacc
     3361 ggcgaggtga tcggaatgcc tcgcagggtg ccgacgtcct ggccgggtag ggaggggcgc
     3421 tcccggacgc tgatgtcttc gtgtcgggat ctgcgtctgc ctcgcactcc gacttcgatc
     3481 cgacctggtc gctcctcgca gatcccatag agcgctcccg cgctgaggtg ggtgaggaac
     3541 gaaccctcgc cacaggccag aaccgccgcc atccaccgtc cctcgcgcga tagctcggag
     3601 cggcccaccg agtagacgcc gcgcctgatc tggtgcaggc ggcccgtctt gagtcggtgg
     3661 cctgtctgct ttgcgctccc gcctttggtg cccccatagg ggcctcaagg gtgggagggc
     3721 gattgggtct ccccctttag gggctggcta cggtgccggc gtcgtggagg cgggcgatct
     3781 cggtcccgtc caggccgagg acctcggcca ggacctgttc ggtgtgctcg cctagttggg
     3841 gggcgcgctg gacggggagg gagccggcgt cgcggaagcg gaggggggag ccgggcatca
     3901 ggtaggtgcc gactccgggg tgctcgacgg cttcgaacat cgggttctcg gtcgagacgc
     3961 gggggtcctc gtcaaccagc tggcggaagg tctggtacgg gccccaggag acgccggtcc
     4021 cgtggaagat ctcgcgcacg gtggcgaggt cgcggccggc gaaccagggg cgcaggatcg
     4081 cggcgatcag gtcgcgggct tcgaagcgac cgctctcggt gctgaggtcg tggccggtag
     4141 cggcttcgat cccacggcag gcctcctcga tcccggtcgc ctcgaccagc gccgcccact
     4201 ggcgggcggt cagggcgacg accatcgccc ggcgcccgtc gccggtctcg aagtcgtggc
     4261 cgaaggcgcc gtagaggtag ttgccgtcct tggcgtggtc gcgcgcgccc agctccgcct
     4321 cggcgatgcg gccgaggttg ccgaccatcg cgaaggcgac gtcggagagc gagagcgaga
     4381 tcagctcgcc cgcgccgccg cgcccgcggc ggcgctcggc ggcgagcagg ccgaccgccg
     4441 ccagggtgcc catcgccacg tcccaggccg gcagcacgct gttcagcggc tcggccaggt
     4501 tgcgcgggcc ggtcgcccag ggaaagccgg tcgccgggtt gacggtgtag tcgacctcgc
     4561 tggtcccgtc cgggttgccg gtcagcgcga ccatgatcag gtcctcccgg cgtgcccgca
     4621 gcgcctcgta gccgagccag ccgcgggccg ggaagttggt caggaagagg ccggcttcct
     4681 cgcccggcgc ggtgatcagc gcgctcgcca gctcgcggcc ctcggggcta cgcaggtcga
     4741 gcgcgatcga gcgcttgccc ttgttcagcc cggcccagaa gaggctctgg ccgtcggcgg
     4801 ccagcggcca gcggccgtgg tcgaggccgc cgccgatctg gtcgaagcgg atcacctcgg
     4861 ccccgagctg ggccagggtc atcccgccga gcggcgcggc gacgaaggcc gagccctcga
     4921 cgacgcgcag cccggccagc ggcccgccgg ccgtcgcggc gctcagctcg ccggcctgta
     4981 gacggtgacc acggcggcgc cgccgaggcc gatgttgtgc tgaagcgcga cctcggcgcc
     5041 gtcgacctga cgctcgcccg actcgccgcg cagctgccag accagttccg agcactgggc
     5101 gagcccggtc gcgccgagcg ggtggccctt cgagatcaac ccgccggagg ggttgaccac
     5161 cgggccggag ccgccgtagg tcgtcgcttc cgcttcgacc agcttctgac cctcgccctc
     5221 ggcggcgaag ccgagcgctt catagctgat cagctcgttg gtgctgaagc agtcgtgcag
     5281 ctcgcagacg tcgacctcgg agggcgcgac cgcggcctcc tcgtaggcct gccgagcagc
     5341 ctcgcggctc atgtcggcgc cgaccaggcg catgcagtcg gtgtcgaccg cgaaggtgtt
     5401 ggagaggtcg gtgacgatcg cctggccggc gatctccacc gcccggtccc agagcccgtg
     5461 ctgctcgacg aagcgctcgc cggcgacgat cgcggcggcc gccccgtcgg aggtcggcga
     5521 gcactgcagc ttggtcaggg gctcgtggat catccgcgag gcctggatct cctccagcgt
     5581 gtactcgtcc tggaactggg cgtaggggtt gttgaccgag tgcttgtggt tcttccaccc
     5641 gatccaggcg aagtggtcgg gggtcgagcc gtagcgctgc atgtggtcgc ggcccgcgtt
     5701 gccgaacatt tccggcgcga acgggtcgtc ctcgggggcg cgcagctctg ccatccgctc
     5761 cttgtggcga ccgatcgggt cggcgcggtc cgcgaagccg acggcgagcg agccgcgctg
     5821 catcttctcg aagccgaccg cgagcgcgca ctcggcgagg ccgccgcgca ctgcctcgcg
     5881 cgccatccag agggccgagg agccggtcga gcagttgttg ttgacgttga cgatcgggat
     5941 ccccgtgagg ccgagcgagt agatcgcgcg ctggccggcg gtcgagtcgc cgtagcagta
     6001 gccggcggcg gcgcgctgca cctgctcgaa gctgacgccg gcgtcctcga gcgccttcga
     6061 accggcctcg ctgacccagt ccggatagtc gccgtccttg ctccccggct tgtcgaactt
     6121 ggtcattccg accccgatga caaaggtccg attgctcatc tcactccctc gattcctctt
     6181 ggaatgcccc gaaaagggct ctacgactgc gtttgactcc cctaacaata acaagatcca
     6241 gcgccgctgc ttaaatcgtg gtatgttgcc gcgcgcagtt gggaagccgc cgcggcggca
     6301 agttcggagg gaaccatgga aagaggagaa atgacgcgac ttcatgtatt gcgagcggca
     6361 cttgtgctcg ccttgacggc gctgctcgcg atcggcgtgg ccgcttgcgg cggcggtgac
     6421 gacagcaccg gtggaggggg cgacaccagc ggcggcagcg ccgaagctga cgcctcggtc
     6481 aaagacgcgg aagcgtattt ggcgaaatac ggcaaaggcg tcggcggtcc gctgccgacc
     6541 gagccggcca aaccgcagcc cggcaaagac gtctggatcc tttcctgcgc ccaggccgcc
     6601 agcggttgcg cctcgatcac ggcggcgatc aaagaagcgg ccgaagaagt cggctggaaa
     6661 gcgaccgtcg tcgacggcgc cggcgacgtc tcgaaatgga actcggcggt cgaccaggcc
     6721 cgggtggccg gcgccgacgc gctgttctcc gtctcggtcg actgcgcccc gatcaaacag
     6781 accctggaac gcgccaaagc ggccgggatg atcaccgagg gcgtctccgc ctacgactgc
     6841 agcgacccga tcagcggggg cggcgaaccg ctcttcaccc acatgttcga aggcccgatc
     6901 aaaggccccg aaagctgggc gaaactggcc gaagccggcg gcggcttgca ggccgcctgg
     6961 accgtcgtcc acgtccccgg cgagaaggaa gcgatcgtct tcacctcgca ggacgtcgcg
     7021 gtcacccagt acatcaacaa agggttcaaa gaccagttca actgcgacga atgctcgatc
     7081 gcgagcgagg tcccgttcca cctgaccgaa ctcggctcgc cgctgacgca gaaagcgcag
     7141 accgcgatgc tgaagtaccc ggacgccaac gtcctggcgc ccccgtacga cccggtctcc
     7201 gaaagcacgg cgcaggcgat cgtcaacgcc ggcaaagtcg gcacggtcta caacaccggc
     7261 ctgctcgcga ccccgtccaa tgtcgagctg atccgctccg ggcgcggtca ggagatgacg
     7321 gtggcctggg actcgcgctg gttcggttgg gccgcggtcg atgacgtgat ccgcctgttc
     7381 aacgaccagg aaccggtcta ctccggttgg gaccagggca tcgtcgacaa agaaaacctc
     7441 cccgaaggcg aggtctacga agcaccggtc gactacatcg ccaactacga aaagctctgg
     7501 ggactggact gagccttgga gcaggtagcg gacaggaccg gcgcaaccgg acctgaggtc
     7561 tcgctgcgcg tgggtgacgt cgagaagttc ttcggcgcca cccacgccct gcggggcgtc
     7621 agcctcgagc tcaaccgctt cgaggttcat ggtctggtcg gcgagaacgg ctgcggcaag
     7681 tcgaccctgg tgaaggtgct ctcaggggtc caccacgccg acgccggttg ggtcgaagcc
     7741 ggcgagaaga aaatctccgc cgaagacgtg accccggccg acgcccgcga gatgggcgtc
     7801 accgtggtcc accaggaggc accgatcttt ccctcgatga cgatcgagga caacctctcg
     7861 cttgggcgcc gctacccgac ccggatgggc cgggtcgacc ggggcgcgat ccgcaagcaa
     7921 gcggaagagg taatcgagca ctacgaaatc cccgccgggc cgcgcactcc cgcgcacgag
     7981 ctgaacccgg cggtgcgggc gatggtggcg atcgcccggg cgctgcgcga cagcgccagc
     8041 cgcgacagcg gcaccctgat cctcgacgag ccgaccgcgg gcctcaacga ggtcgaagcc
     8101 gacgacctgc tggaagcggt gcggcgctac gccgcccgcg ggcaggcgat catgctggtc
     8161 tcgcaccggc tcgacgagat cgtcggcgcc tgccagcggg tcacggtgat gcgcgacggc
     8221 gtcgtcgtca ccaccctgca tggcgccgag atcgaccgcg gcaacatcgt ccgccagatg
     8281 gtcggctcga acctcgcgaa ggagtacgag gccgacgccg cgcgggtcgc cgaggcttcc
     8341 gccaacccgg tcgtgctgca gttcgacggg gtcagcactg accagctgca ggacgtctcg
     8401 ctggccgcgc acgagggcga gatcctcggc ctcgccggcc agctcggcgc cggctgctcg
     8461 gaggcgctgc agatcgccta cgggatcgct ccttataagg ggggcaaggt gaccctcgcc
     8521 gggcgccgcc tccccagccg ccgtgccggc cgcgctcagt ccgacctggt cggctacgtg
     8581 cccgccgacc ggcccggcga aggcatgttt ggcggtcgct cggtgcgcga gaacctcggg
     8641 gtcgccgaga accgccgcta ctggcgcggc ggcttcttcc gccgcggcgc cgaaagccgt
     8701 gactcggaag cgctgatcga gcagttccgg atcaagccgg acgacccggg cgcgctgatg
     8761 accaacctct ccggcggcaa ccagcaga
//
LOCUS       DASRIG010000145         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_595198, whole genome shotgun sequence.
ACCESSION   DASRIG010000145 DASRIG010000000
VERSION     DASRIG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595198"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFB52_03990"
     CDS             <1..508
                     /locus_tag="VFB52_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZO05523.1"
                     /translation="VYPLGDMPAAVLAVRAEAKRLRQKFGPSQVYAYGSSAGGTLAAL
                     LSGDGLVGGAAAKAPVSNLVSWEWPVEHYGPLYWDRAQLDLKARYRLSPLRRAAKQPL
                     LLVHGIDDQVVPISQSEAYANKFKRVYLWEVLGGHHAERSQPEVLQRTLIWLAEIAEA
                     QAQGLIPR"
     gene            534..2597
                     /locus_tag="VFB52_03995"
     CDS             534..2597
                     /locus_tag="VFB52_03995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZO05524.1"
                     /translation="MNSVNKSRTTKTGTGFRPDIEGMRAIAIIAVLLCHAGIPFAAGG
                     YVGVDVFFVISGFLITRLLLNELGRNDTISIPGFYARRIKRLLPLSAVLLVTVAILTV
                     LILSPVRAVEVAGDLISSAFYVANWHFAAQSVDYFAQDIEPSPVQHLWSLAIEEQFYL
                     VWPSLILGVTWFARNRSGRSLRPILLGTFAFIFAASLAFGIYYTNAEPTAAYFNTFGR
                     AWELALGALLALVGVVAMPRIAATLLSWIGVGAIVYAILAFDETTPFPGSAALIPTLG
                     AAALILAGGALYERAKGTPIGVLSTAPARYVGRISYSWYLWHWPLLIFAAALWGPLSV
                     AAGIAVVAASWVPTAITHVLIEEPFRRSKKLVLMPRRAFALGAACMAIVVGAAFWLES
                     DQPNVDTAPISEVKGAAALAEQRVPQDRAVALRPSPVDARDDRSEAFYDGCLVGVAGT
                     NSNRCLYGDPDGTKTLILFGDSHALQYFAPLEKLALENKWRLIVLTKAECSPGEVRIM
                     SMVEEREYSQCEAWREETLERIEEAGERAMVVMSGDTAYVPLDEGGNELSGKEASEEL
                     ERGYLKTIDRIHKSGLQVTVIRDTPASANDVPSCVSEDLENLDKCAFERVRDWNLEFD
                     VRAAEKSPFARLIDITDEICPSDLCRAVIGDALVYRDKTHLTATFARTLSPWIGEGIK
                     EAELF"
     gene            complement(2598..2771)
                     /locus_tag="VFB52_04000"
     CDS             complement(2598..2771)
                     /locus_tag="VFB52_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZO05525.1"
                     /translation="MPFVEVHWYEGRSDEQKAEIAKRIEAALIEVAGVTPEHCWVKFS
                     DSSKTDFIIGESK"
     gene            complement(2796..3122)
                     /gene="trxA"
                     /locus_tag="VFB52_04005"
     CDS             complement(2796..3122)
                     /gene="trxA"
                     /locus_tag="VFB52_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZO05526.1"
                     /translation="MSGKLSDVTDATFKADVIDADGPVLVDFWAPWCGPCRVVHPVLE
                     EIDAERDDLKIVSLNVDENQQTAMEYEVLSIPTMILFKDGAMAKKVVGAMPKRRLEAE
                     LEPALA"
     gene            3387..>3568
                     /locus_tag="VFB52_04010"
     CDS             3387..>3568
                     /locus_tag="VFB52_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05527.1"
                     /translation="MALTLAVYPHWRTIPELGREIGGSGALTRAILELVTLGLLESHG
                     TAVRPTKPVAYLERLML"
BASE COUNT          563 a         1282 c         1128 g          595 t
ORIGIN      
        1 ggtctacccg ctcggcgaca tgccggcggc ggtgctggcg gtgcgggcgg aagcgaaacg
       61 gctgcgccag aagttcggcc cgtcgcaggt ctacgcctac ggctcctcgg ccggcggcac
      121 gctcgccgcg ctgctctccg gcgacgggct ggtcggcggc gccgccgcca aggcgccggt
      181 ctccaacctg gtcagctggg aatggccggt cgagcactac gggccgctct actgggatcg
      241 ggcccagctc gacctgaagg cgcgttaccg gctctcgccg ctgcgccgag cggccaagca
      301 gccgctgttg ctggtccacg ggatcgacga ccaggttgtg ccaatctccc agagcgaggc
      361 ctacgcgaac aagttcaaac gcgtctatct gtgggaggtc ctgggcggcc accacgccga
      421 gcgctcgcag ccggaagtcc tgcaaaggac gctgatctgg ctcgcggaaa tcgccgaagc
      481 ccaggcccag ggcctgatcc cgcgctagat cgccctaaca tttccccttt cctatgaact
      541 cggtcaacaa aagccggacg acgaagaccg gcaccggttt ccgccccgac atcgaaggga
      601 tgcgggcgat cgcgatcatc gccgtgctgc tctgccacgc cggcatcccg ttcgcggccg
      661 gcggctacgt cggggtcgac gtcttcttcg tcatctccgg cttcctgatc acccgcctgc
      721 tgctcaacga gctcggccgc aacgacacca tctcgatccc cggcttctac gcgcgccgga
      781 tcaaacgcct gctgccgctc tcggcggtgc tgctggtcac ggtcgcgatc ctcaccgtgc
      841 tgatcctctc gccggtgcgc gcggtcgaag tcgccggcga cctgatcagc tccgccttct
      901 acgtcgccaa ctggcacttc gccgcgcagt cggttgacta cttcgcccag gacatcgaac
      961 cgagcccggt ccagcatctc tggtcgctgg cgatcgagga gcagttctac ctcgtctggc
     1021 cgtccctgat cctcggcgtc acctggttcg cccgcaaccg cagcggccgc tcgctgcgcc
     1081 cgatcctcct gggcaccttc gccttcatct tcgccgcctc gctcgccttc ggcatctact
     1141 acacgaacgc cgagccgacc gccgcgtact tcaacacctt cggccgcgcc tgggagctcg
     1201 cgctcggcgc gctgctggcc ctggtcgggg tggtggcgat gccgcggatc gcggcgaccc
     1261 tgctcagctg gatcggggtg ggggcgatcg tctacgcgat cctcgccttc gacgagacga
     1321 ccccgttccc gggctcggcg gcgctgatcc cgacgctcgg ggcggcggcg ctgatcctcg
     1381 ccggcggcgc cctctacgag cgcgccaaag gaaccccgat cggggttctc tcgaccgcgc
     1441 cggcccgcta cgtcggccgc atctcctact cctggtacct ctggcactgg ccgctcctga
     1501 tcttcgccgc ggccctctgg gggccgctct cggtcgccgc cgggatcgcc gtcgtcgccg
     1561 cttcctgggt cccgaccgcg atcacccacg tgctgatcga ggagccgttc cgccgctcga
     1621 agaagctggt cctgatgccg cgccgcgcct tcgccctcgg cgccgcctgc atggcgatcg
     1681 tggtcggcgc cgcgttctgg ctggaaagcg accagccgaa cgtggatacg gcgccgatct
     1741 ccgaggtcaa aggcgccgcc gccctggccg aacagcgagt cccccaggac cgtgccgtcg
     1801 ccctgcggcc gagcccggtg gacgcccgcg acgaccgctc cgaggcgttc tacgacggct
     1861 gtctggtcgg cgtcgccggg accaactcca accgctgcct ctacggcgac ccggacggga
     1921 cgaagacgct gatcctgttc ggcgactcgc atgccctgca gtacttcgcg ccgctggaga
     1981 agctggcgct ggaaaacaaa tggcgcctga tcgtcctgac caaggccgaa tgctcgccgg
     2041 gcgaagtgcg gatcatgagc atggtcgaag agcgcgagta ctcccagtgc gaggcctggc
     2101 gcgaggagac gctggaacgg atcgaagaag ccggggagcg ggcgatggtg gtgatgagcg
     2161 gcgacaccgc ctacgtcccc ctcgatgaag gcggcaacga gctgagcggc aaagaggcct
     2221 cggaagagct cgagcgcggc tacctgaaga cgatcgaccg gatccacaaa tccggcctcc
     2281 aggtgaccgt gatccgcgac acccccgcct ccgccaacga cgtccccagc tgcgtctccg
     2341 aagacctcga gaacctcgac aagtgcgcct tcgaacgggt ccgcgactgg aacctcgaat
     2401 tcgacgtccg cgccgccgaa aaatccccct tcgcccgcct gatcgacatc accgacgaga
     2461 tctgccccag cgacctctgc cgcgccgtga tcggcgacgc gctcgtctac cgcgacaaaa
     2521 cccacctcac cgccaccttc gcccggacgc tgagtccgtg gatcggcgaa gggatcaagg
     2581 aagcggagct gttctagcta cttcgactcg ccgatgatga agtcggtttt cgaggagtcg
     2641 gagaacttca cccagcagtg ctcgggagtc accccggcaa cctcgatcag cgccgcttcg
     2701 atccgcttgg cgatctcggc cttctgctcg tcggaacggc cctcgtacca gtggacctct
     2761 acgaacggca tggcgggtgg ggagctggcg ccgcgctagg cgagcgccgg ctccagctcg
     2821 gcctcgaggc gacgcttggg catcgcgccg acgactttct tcgccatcgc cccgtctttg
     2881 aagaggatca tcgtcgggat cgagagcact tcgtactcca tcgccgtctg ctggttctcg
     2941 tcgacgttga gggagacgat cttgaggtcg tcgcgctcgg cgtcgatctc ctcgaggacc
     3001 gggtggacga cgcggcaggg accgcaccac ggagcccaga agtcgacgag cacagggccg
     3061 tcggcatcga ttacgtccgc tttgaaggtg gcgtcggtta cgtcgctcag ttttccgctc
     3121 actggtcccg tctcccaaat cgaggttcgt agtccaatac ttcagaaagt atagcgcctg
     3181 cctgcaaagg tggtgggtca gtttgaatcg gcgccccatc tgaagctgca tccccgctag
     3241 cttcgccgcc accaagacaa gaaagtggcg ggggcgctgg tggaacagcc ccccgccgtg
     3301 gcaccgaaag gcggaacctt ccgatgcagg cgaatcgtac gcggcggcac tggccagccg
     3361 accgcatccc cctccagcgg gcggtcatgg ccttaacgct cgcggtctac ccgcactggc
     3421 gcacgatccc cgagttgggc cgggagatcg gcggctccgg cgcgctcacc cgagccatct
     3481 tggagctggt cacgctcggc cttctggaaa gccacggcac cgccgtgcgc cccaccaaac
     3541 ccgtcgctta tctggagagg ctgatgct
//
LOCUS       DASRIG010000146         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_66697, whole genome shotgun sequence.
ACCESSION   DASRIG010000146 DASRIG010000000
VERSION     DASRIG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66697"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFB52_04015"
     CDS             complement(<1..654)
                     /locus_tag="VFB52_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HZO05528.1"
                     /translation="MSQQRIRRRQRRRGGIGGKLALAFGALFAVVAIAAIAVTSWVLD
                     VAADAPSLSSCKKVDKRGNTTIYAADGTRLGRVASNETHIPVSLDQIPKTLQHATVAI
                     EDKRFYEHGGYDTEAIVRALRKNFEAGEVVEGASTITQQLVRNLCIRNPEQTVERKII
                     EIELATEYAKRHSRKQILDSYLNVATYGTVEGATAVGVGAAARIYFSKPVAKLNLTES
                     "
     gene            complement(694..1149)
                     /locus_tag="VFB52_04020"
     CDS             complement(694..1149)
                     /locus_tag="VFB52_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZO05529.1"
                     /translation="MSPEVSQVSRSTRTRLRLLRRLIVASVVVVLAMIALAQFTDLER
                     LATGVLASSAVIALIVGFAAQHTLGNLVAGLQIGVTQPFRIGDKISFEENEGRVTDIS
                     LAYTYIDPGDGSTYVIPNQLLVDGIVHNHSTGTGQTSEHPAVPPPEQPS"
     gene            1148..1858
                     /locus_tag="VFB52_04025"
     CDS             1148..1858
                     /locus_tag="VFB52_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZO05530.1"
                     /translation="MEANLAAPEVERPPERDFPYANWGPGAAVLGVVMAIGAGILLGI
                     PAAFFPKEPDGDLGAAANTVAQLGLVAGFLLVPMAIASVRGATDIGEILRRLGVRAFE
                     LSALKWMAATAGAYLLFAGLYSTLILEPEQEDIAESFGPVPVQIVLIVFGAAIAEELC
                     FRGFLFGGLREKLPRYWAALIAGLIFGSLHALTGISAVPPLIAFGFFLCLLYEKTGSI
                     VPGIILHMLNNSVALLAQ"
     gene            1871..2842
                     /locus_tag="VFB52_04030"
     CDS             1871..2842
                     /locus_tag="VFB52_04030"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZO05531.1"
                     /translation="MIRRFALILAALTLLALPASAAAATPPSKEPVKEVVPDATVKIK
                     LGNLKGKRAEIFSTVPVFGTVKPWVPGQTVKVEFFLDGKELVTKKANVTKGGGNFGKF
                     KSSVIVREDGRYTVSAKHEATDEQGGGSTVRKLWRVSFPSLRQGDCGKAVVGFKKAMR
                     EMGYIANSGSCYGGKTARGVLAYRKVNNMSRSMRSGAALVKAVYDGQGEYHVKYPGAG
                     QHVEAPLSKQVLVFADGDKPTAIYPISSGKSSTPTVTGHYEFIRTEPGYNSHGMYYSF
                     YFYGGYAVHGYESVPDYPASHGCLRTFIADQPEIYNRINFGEDIFIW"
     gene            2880..>3323
                     /locus_tag="VFB52_04035"
     CDS             2880..>3323
                     /locus_tag="VFB52_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05532.1"
                     /translation="MSGGTKMRVFVAGASGVLGRPLVRQLLAAGHRVTGITRREEAAE
                     ELRAAGAEAVVCDVYDADALREAMVAAKPEAVVHVLTALPQRFKPREDYLGPTNRVRT
                     EGTRNLVAAARAAGAKRLIAESVAFFYEPVGGWVKDEGAPLMGDPP"
BASE COUNT          496 a         1112 c         1163 g          552 t
ORIGIN      
        1 cgactcggtc aggttgagct tcgcgaccgg tttcgagaag tagatccgcg ctgcggcccc
       61 gactccgacc gcggtcgcgc cctcgaccgt gccgtaggtg gcgacgttga ggtaggagtc
      121 gaggatctgc ttgcgagagt ggcgcttggc gtactcggtt gccagctcga tctcgatgat
      181 cttgcgctcg accgtttgct ctgggttgcg gatgcagagg ttgcggacca gctgctgggt
      241 gatcgtcgag gcaccctcga ccacctcgcc ggcttcgaag ttcttgcgca gggcgcggac
      301 gatcgcctcg gtgtcgtagc cgccgtgctc gtagaagcgc ttgtcctcga tcgccacggt
      361 cgcgtgctgc agcgttttcg gaatctgatc gagcgagacc gggatgtggg tctcgttgga
      421 ggcgacgcgg ccgaggcggg tcccgtcggc cgcgtagatc gtcgtgttgc cgcgcttgtc
      481 gaccttcttg cagctcgaga gagacggtgc gtcggcggcg acgtcgagca cccaggaggt
      541 gaccgcgatc gccgcgattg cgaccaccgc gaagagcgcg ccgaaggcca gcgccagctt
      601 gccgccgatc cccccgcggc gtctctgcct ccgccggatg cgctgttgcg acatcggctc
      661 agagggtact tcgccggccc gcgcggctcc ggctcagctt ggctgctctg gaggcgggac
      721 cgccgggtgc tcggacgtct ggccagtccc ggtcgagtgg ttgtggacga tcccgtcgac
      781 cagcagctgg ttggggatca cgtaggtgct gccgtcgccg gggtcgatgt aggtgtaggc
      841 gagggagatg tcggtgaccc gaccctcgtt ttcttcgaag ctgatcttgt cgccgatccg
      901 gaacggctgg gtgacgccga tctgcagccc cgcgaccagg ttgccgaggg tgtgttgggc
      961 ggcgaaaccg acgatcagcg cgatcaccgc cgaggaggcg agcacgccgg tggcgaggcg
     1021 ctcgaggtcg gtgaactggg ccagcgcgat catcgccagc acgacgacga ccgaggcgac
     1081 gatcagccgg cgcagcaggc gcagccgggt tctggtcgag cgggagacct gtgagacttc
     1141 cggcgacatg gaggccaact tagctgcgcc ggaggtcgag cgcccgcccg aacgggactt
     1201 cccctacgcc aactgggggc cgggggcggc ggtgctcggg gtggtcatgg cgatcggcgc
     1261 ggggatcctg ctcgggattc ccgctgcctt ctttcccaag gagcccgacg gcgacctcgg
     1321 cgctgccgcc aacacggtcg cccagctggg tctcgtcgcg gggttcctgc tggtgccgat
     1381 ggcgattgcc tcggtacgcg gcgcgacgga catcggggag atcctgcgcc ggctgggcgt
     1441 gcgtgccttc gagctgtcgg cgctcaagtg gatggccgcc acggccggcg cctacctgct
     1501 cttcgccggc ctctactcga ccctgatcct cgaacccgag caggaagaca tcgccgagag
     1561 cttcgggccc gtgccggtgc agatcgtcct catcgtcttc ggcgccgcaa tcgccgagga
     1621 gctctgcttt cgcggctttc tcttcggtgg cttgcgcgag aagctgccgc gctactgggc
     1681 ggcgttgatc gccggcctga tcttcggctc cctgcacgcg ctcaccggga tcagcgcggt
     1741 gccgcccctg atcgccttcg gcttcttcct ctgcctgctc tacgagaaga ccggctcgat
     1801 cgtgcccggg atcatcctgc acatgctgaa caactcagtc gccctgctcg cccagtagag
     1861 ttcccggccc gtgattcgcc gcttcgccct gatcctcgcc gcgctgacgc tgctcgcgct
     1921 ccccgcctcg gccgctgccg cgacgccgcc gagcaaggag ccagtcaagg aggtcgtgcc
     1981 ggacgcgacc gtgaagatca agctcgggaa cctgaagggc aagcgcgccg agatcttctc
     2041 caccgtcccc gtcttcggca cggtcaagcc ctgggtgccg ggccagaccg tcaaggtcga
     2101 gttcttcctc gacggcaagg agctggtcac caagaaggcc aacgtgacta agggcggcgg
     2161 caacttcggc aagttcaagt cgagcgtgat cgtccgcgag gacggccgct acacggtcag
     2221 cgccaagcac gaggcgaccg acgagcaggg cggcggcagc accgtgcgca agctctggcg
     2281 ggtcagcttc ccgtccctgc gccagggcga ctgcggcaaa gccgtggtcg gcttcaagaa
     2341 ggcgatgcgg gagatgggct acatcgccaa cagcggctcc tgctacggcg gcaagaccgc
     2401 gcgcggcgtg ctcgcctacc gcaaagtcaa caacatgagc cgctcgatgc gctcgggtgc
     2461 tgccctggtc aaggccgtct acgacggcca gggcgaatac cacgtgaaat acccgggtgc
     2521 cggccagcac gtcgaggcgc cgctctccaa gcaggtcctt gtcttcgccg acggcgacaa
     2581 gccgaccgcg atctacccga tctcctccgg caagtcctcg accccgacgg tgaccggtca
     2641 ctacgagttc atccgcaccg aaccgggcta caactcgcac gggatgtact actcgttcta
     2701 cttctacggc ggctacgcgg tgcacggcta cgagtcggtg cccgactatc ccgccagcca
     2761 cgggtgcctg cgcaccttca tcgccgacca gccggagatc tacaaccgga tcaacttcgg
     2821 cgaagacatc ttcatctggt agcaaccacc gcgcccccgc gccgcgctaa tttgggggca
     2881 tgagcggggg tacgaagatg cgggtcttcg tcgccggcgc cagcggggtg ctgggacggc
     2941 ccttggttcg gcaactgctc gccgccggcc accgggtgac cgggatcacc cggcgcgagg
     3001 aggcggccga ggagctgcgc gcggccgggg ccgaggcggt cgtctgcgac gtctacgacg
     3061 ccgacgcgtt gcgcgaggcg atggtggctg cgaagccgga ggcggtggtg cacgtgctga
     3121 ccgcgctgcc gcagcgcttc aagccgcgcg aggactacct ggggccgacc aaccgggtgc
     3181 gcaccgaggg cacccgcaac ctggtcgcgg cggcccgtgc ggcgggggcg aagcggctga
     3241 tcgccgagag cgtcgccttc ttctacgagc cggtcggagg ctgggtcaag gacgagggtg
     3301 cgccgctgat gggcgacccc ccg
//
LOCUS       DASRIG010000147         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2143129, whole genome shotgun sequence.
ACCESSION   DASRIG010000147 DASRIG010000000
VERSION     DASRIG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143129"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="VFB52_04040"
     CDS             <1..1669
                     /locus_tag="VFB52_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZO05533.1"
                     /translation="RAGAARAARALAELIETEDESERELRSTTARIAIRKTRSDFVSL
                     QHRPSGTGGRTAALARLIEDLAWIRRFGAQTPDPVQADPAIASAREQIEGGVPAVLRQ
                     AAGQLEGTVPLTDGAPGGPGGDVVEELDRAHDDLGSAALARMAELAEQGEDAEQQAAR
                     ELDAVFRLRLLSFITAEVASTTRRALGAPAGARVEAARARLDAAGRLARAHASMRSVW
                     LRNSLRGGLGLGLAVLIGQLADIQNAFWVVLGTLSVLRSSALATGSTIVWSLLGTFAG
                     IVVGGLLVIAVEGDETALWILLPFATGLAAYTPRAVSFAAGQAGFSIVVLVLFNLIHP
                     SGWEVGLLRIEDVAIGAGVSLLVGLLFWPRGAVSVLRRAMGAAYTRAAAYLDATVEAL
                     LAGESPPVEAEREAFGTAQVLDSGIRDYLSDRSGATAPIEELAVLMAGAVRVGEVAGL
                     LYHAGVLIQIEPVDPGLGRVDAARRELEADRDARHRWYTELGKAIAARQPAPEPEPTA
                     TASAPVVLERTPGDAGVPPGVAIAWARRYLDALRELEQPLARAANTP"
     gene            complement(1673..2638)
                     /locus_tag="VFB52_04045"
     CDS             complement(1673..2638)
                     /locus_tag="VFB52_04045"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HZO05534.1"
                     /translation="MPSVPEYVHTPGHHCGSTALRNLLAFHGVELSEEMAFGLGAGAG
                     FYYLAMEDASPSRWFNGRTARLEETFNELTGATLRMRTFGEDDAAAWAAARAEVDAGN
                     PALLLTDLYYLDHYGNSAHFPGHAVVLAGYDEEVAYLSDTGFEELQRTRLENLAKARH
                     SSHPAFALDGHMFTVEGAVDRARLEAAIPAAIERAATEMLEPPFGEFGGLPAVERLAA
                     EAGAWPETVEDWQWCARFAYQVIERRGTGGGCFRPMYSRFLAEAGREEAPLAAEAAVR
                     WTELADAFRAASESDEPVAELWSAVGAAGARVAESERRLWTALGS"
     gene            complement(2643..4070)
                     /locus_tag="VFB52_04050"
     CDS             complement(2643..4070)
                     /locus_tag="VFB52_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HZO05535.1"
                     /translation="MTGSPGEALPFSYEEFSEWFDDYEGRFLHPALTTAITALDQYLD
                     EELQDPQRVRVRITPGRVKSRQRAWQKLNDKYGDKVGSLADVPKVVDDLVGLRIVCTN
                     KSDMGRVVEMLEALDEYSDGEHPVLAVVPESVNDWRSSPKESGYRAYHVNLCTSVSQV
                     TKRHPVICELQVRTLLQDSWGELTHEDTYKPGATDVPPLMDTLSRRMADLMASIDDIA
                     EDLRSEIDSQEADSLGANGKTTDDREPPAKQKASSNPTREAAEAYLAELTVALKRPTP
                     LATLAWELQREFGSDLSKDWAGHGTFRAMLESAVPQARVSGGPPVWVLPADFDVSAYD
                     GGHPDFPRVVSLLKDADPSFPLIPSELWPRVYAVLAEATQAGVWESEQEPLSKLMKHA
                     RDASMDNFGFALSRRQLNYVANALLSKKQLTPGMTAEQIEEAFVAWMLKRSSDIGISR
                     DDADQIAAWLRGQGPEASQVPESKS"
     gene            4096..4590
                     /locus_tag="VFB52_04055"
     CDS             4096..4590
                     /locus_tag="VFB52_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZO05536.1"
                     /translation="MEKLDLRLLRYMRTHGHSPGMEGVAKALGKAGNNGLVWVVLGLV
                     LALIDPDHREEWLICAVLGPVAIVLNYGIKLLVKRPRPVLEGLPPLGGAPSSLSFPSA
                     HALSSFAVATAMFRVDPASAGALVVALAISICRPYLGMHYPSDVLAGAFLGVLLGLIV
                     PLSI"
BASE COUNT          612 a         1671 c         1614 g          740 t
ORIGIN      
        1 ccgcgccggc gccgcccgcg cggcgcgcgc cctcgccgag ctgatcgaga ccgaggacga
       61 gtccgagcgc gagctgcgca gcaccaccgc ccggatcgcg atccgcaaga cccgcagcga
      121 cttcgtctcg ctgcagcacc gccccagcgg aaccggcggg cgcaccgcgg cgctggcgcg
      181 gctgatcgag gacctcgcct ggatccgccg cttcggtgct cagacccccg acccggtgca
      241 agccgacccc gcgatcgcca gcgcgcgcga gcagatcgaa ggcggtgtgc ccgcggtgct
      301 gcgtcaggcc gctgggcagc tcgagggcac cgtgccgctc accgacgggg cgcccggcgg
      361 tcccggcggc gacgtggtcg aggagctcga ccgggcgcac gacgatctcg gcagcgcggc
      421 gctcgcccgg atggcggagc tggccgagca gggcgaggac gccgagcagc aggccgcgcg
      481 tgagctcgac gcggtcttcc gcctgcgcct gctctccttc atcaccgccg aggttgcgag
      541 taccacccgg cgcgcgctcg gcgccccggc cggtgccagg gtggaagcgg cccgtgctcg
      601 gctcgacgcc gccggccggc tcgcccgcgc gcacgccagc atgcgctcgg tctggctgcg
      661 caacagcctg cgcggcgggc tcgggctggg gctggcggtg ctgatcggcc agctcgccga
      721 catccagaac gccttctggg tcgtgctcgg cacgctctcg gtgttgcgct ccagcgccct
      781 cgcgaccggc tcgacgatcg tctggtcgct gctcggcacc ttcgccggga tcgtcgtcgg
      841 cggcctgctc gtgatcgcgg tcgagggcga cgagacggcg ctctggatcc tgctcccgtt
      901 tgccaccggg ctggccgcct acaccccgcg cgcggtctcc ttcgccgccg gccaggccgg
      961 cttctcgatc gtcgtcctcg tgctcttcaa cctgatccac cccagcggct gggaagttgg
     1021 tctgctgcga atcgaggacg tcgcgatcgg cgccggcgtc agcctcctgg tcggcctgct
     1081 gttctggccg cgtggcgcgg tcagcgtgct gcgccgggcg atgggcgccg cctacacgcg
     1141 tgccgccgcc tacctcgacg cgacggtcga ggcgctgctg gctggcgagt caccgccggt
     1201 cgaggccgag cgcgaagcct tcgggaccgc ccaggtgctc gacagcggga tccgcgacta
     1261 cctctccgac cgcagtggcg ccaccgcccc gatcgaggag ctggcggtgc tgatggcggg
     1321 ggctgtgcgg gtgggtgagg tcgccgggct gctctaccac gccggggtcc tgatccagat
     1381 cgagccggtc gacccgggcc tgggacgcgt cgacgccgcc cgccgcgagc tggaagccga
     1441 ccgcgacgct cgccaccgct ggtacaccga gctcggcaag gcgatcgccg cccgtcagcc
     1501 cgccccggag cccgagccca ccgccacggc gagcgccccc gtcgtgctcg aacgcacccc
     1561 cggcgacgcc ggcgtcccgc ctggtgtcgc gatcgcctgg gcccgccgct atctcgacgc
     1621 cctgcgcgag ctcgagcagc cgctcgcccg cgccgcgaac acgccctagt agttacgagc
     1681 cgagcgcggt ccagagacgg cgctcggact cggcgacgcg cgcgcccgcc gcgccgaccg
     1741 cgctccacag ctcggcgacc ggctcgtcgc tctcactggc cgcgcggaag gcgtcggcca
     1801 gctcggtcca gcgcaccgcc gcctcggccg cgagcggggc ctcctcgcga ccggcctcgg
     1861 cgaggaagcg cgagtacatc ggccggaagc agccgccgcc ggtgccgcgg cgctcgatca
     1921 cctggtaggc gaagcgggcg caccactgcc agtcctcgac cgtctccggc caggcgccgg
     1981 cctcggcggc gagccgctcg accgcaggca ggccgccgaa ctcgccgaac ggtggctcga
     2041 gcatctcggt cgcggcgcgc tcgatcgccg ccgggatcgc cgcctccagc cgcgcccggt
     2101 cgaccgcgcc ctcgaccgtg aacatgtggc cgtcgagggc gaaggcggga tggctggagt
     2161 ggcgcgcttt cgccaaattc tccagccgcg tccgctgcag ctcctcgaag ccggtgtcgg
     2221 agaggtaggc gacctcctcg tcatacccgg ccagcacgac cgcgtgcccc gggaagtggg
     2281 ccgagttgcc gtagtggtcg aggtaataga ggtcggtgag caggagggcc gggttgccgg
     2341 cgtcgacttc ggcccgcgcc gccgcccagg cggccgcgtc gtcctcgccg aaggtgcgca
     2401 tccgcagggt cgccccggtc agctcgttga aggtttcctc gagccgcgcg gtacggccgt
     2461 tgaaccagcg gctcggcgag gcatcctcca tcgccaggta gtagaagccg gccccggcgc
     2521 cgagcccgaa ggccatctcc tcgctcagct cgaccccgtg aaaggcgagc agattgcgca
     2581 gcgcggtcga gccgcagtgg tggcccggcg tgtgtacgta ttcgggtacg gacggcacgt
     2641 ggtcagctct tgctctctgg cacttgggat gcctcgggtc cttggccgcg cagccacgcc
     2701 gcgatctggt cggcatcgtc ccgggatatc ccgatgtcgc tggaccgttt gagcatccag
     2761 gcgacgaagg cctcttctat ctgctcggcg gtcatgcccg gggtgagctg ctttttcgac
     2821 agcagggcat tcgccacata gttgagctgc ctgcgcgaga gggcaaagcc gaagttgtcc
     2881 atcgaggcat cccgcgcgtg tttcatcagc ttgctgagcg gctcttgctc cgattcccag
     2941 acacccgctt gggtcgcttc ggcgaggact gcgtacacgc gcggccacag ctcactggga
     3001 atgaggggga acgatggatc ggcgtccttg agcagggaga cgactcgtgg gaagtcgggg
     3061 tgcccgccgt cgtaggcgga cacgtcgaaa tcggcgggga ggacccagac cggtgggccg
     3121 ccgctgactc gagcctgtgg aacggcgctc tcgagcatcg ccctgaaggt tccgtgaccc
     3181 gcccagtcct tggagagatc gctgccgaac tcgcgctgca gctcccaagc gagcgtggcc
     3241 aacggggtcg gtcgtttcag cgccacggtc agctcggcca ggtatgcctc ggcggcctca
     3301 cgggtcgggt ttgagctggc cttttgcttc gctggcggct ctcggtcgtc ggtcgttttt
     3361 ccgtttgcgc caagtgagtc agcctcttgg ctgtcgatct cggatcgaag gtcctcggcg
     3421 atgtcgtcga tcgacgccat caggtcggcc attcgccgac tcagcgtgtc catcagagga
     3481 gggacgtcgg tggcgcccgg cttgtaggtg tcctcgtggg tcagctcgcc ccagctgtcc
     3541 tggagcaggg tgcggacctg cagctcgcag atcaccgggt gtcgcttcgt gacttgggag
     3601 accgacgtgc acaggttgac gtggtacgcg cggtacccgg attccttggg gctgctgcgc
     3661 cagtcgttga ccgattcggg aacgacagcg aggaccgggt gctcgccgtc ggagtactcg
     3721 tccaaggcct cgagcatctc gaccacgcgg cccatgtctg acttgttcgt gcagacgatc
     3781 cgcagcccga ccagatcgtc gacgaccttc gggacatcgg ccagcgagcc gaccttgtcc
     3841 ccgtatttgt cgttcagctt ctgccaggcg cgctgccgcg acttgacccg gcctggagtg
     3901 atgcggaccc tgactcgctg ggggtcctgc aactcctcgt cgaggtactg gtccagcgcc
     3961 gtgatcgcag tggtcagggc cggatgtagg aagcgtccct cgtaatcgtc gaaccactcg
     4021 ctgaattcct cataggagaa aggcagcgcc tcccctgggc tccccgtcat tcgcgccagc
     4081 ttagactctc gcgccgtgga gaagctcgac ctcagactgc tgcgctacat gcgcacccat
     4141 ggccactcgc ccgggatgga gggcgtggcg aaggcgctcg gcaaggccgg caacaacggc
     4201 ctggtctggg tcgtgctcgg cctcgtcctg gcgctgatcg atccggacca ccgcgaggaa
     4261 tggctgatct gcgcggtgct cggcccggtg gcgatcgtcc tcaactacgg gatcaagctg
     4321 ctggtcaaac ggccgcgccc ggtgctggag gggctgccgc cgcttggcgg cgcgcccagc
     4381 tcgctcagct tcccctccgc ccatgcgctc tcctcgttcg cggtcgcgac cgcgatgttc
     4441 cgggtcgacc cggcgagcgc cggcgcgctg gtcgtcgcct tggcgatctc gatctgccgc
     4501 ccctacctcg gcatgcacta cccctccgac gtgctggcgg gcgccttcct cggcgtcctg
     4561 ctcggtctga tcgtgccgct ctctatatga cccccgaacc acaactgaaa cggagccccc
     4621 ggtgaaggtc ggaatcg
//
LOCUS       DASRIG010000148         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1129093, whole genome shotgun sequence.
ACCESSION   DASRIG010000148 DASRIG010000000
VERSION     DASRIG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129093"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFB52_04060"
     CDS             <1..743
                     /locus_tag="VFB52_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05537.1"
                     /translation="GERTRLFHWSYPGLFDEERATFLVWRQGGAVAAVFAAGGDGAAN
                     DATALALAQRQQHHLEEPEPFSPAEFDDTEVPLENPAIEIPIYWLGRDLAARSGLLAL
                     HLQNTSSSSPAETGRPRAGTYYEDRPLRARGEGVYVDVYSAAQWKRFKARGHLPGALE
                     CGATAHPIELPQGHAVAFHGRARLFRRCDRREPEPTEWVLRARVGGVVVTASTIESCA
                     TCAEPGRGPYNSYKGMRAIARGLELRSR"
     gene            744..1988
                     /locus_tag="VFB52_04065"
     CDS             744..1988
                     /locus_tag="VFB52_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05538.1"
                     /translation="MGNSTGFRIGGIAVLCVAAALVVALVVTDTSGDAAYMPPNVDQT
                     EMVLRLSDLPPGYVNGYLGEGFDDGDPRCSAVKDPPGAPARLSEFVEAYHPSGCVAAY
                     AARWTFPGEPSVASTVASGVLALGSAEAVDAAWEAAPTVLLGRVFPRSEPTEVEPQAM
                     VGVRTRLFHTRATLPYSYGGNDKASFLIWQTGNTMATVMAVGRSYATTDRVAAELAPR
                     QTKRIRKPTPFRQSERYDGEVGLDDPQIDVPVYWLGRTFKPGNGMPPNELFESYWRGK
                     PEAEKSGSGRFFSPQAPAGPLTIRYYNFRLDTWTPSTWRVFAGSKSARVLTTWECTEI
                     RTIKLPGGSATIFGGYARDFKDCPSKPPSVFTAWVNLGGVKVVVNPPESPDGGLFSTP
                     YSSFAGMEAIARGLKLREQPLF"
     gene            complement(2001..2876)
                     /gene="sucD"
                     /locus_tag="VFB52_04070"
     CDS             complement(2001..2876)
                     /gene="sucD"
                     /locus_tag="VFB52_04070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZO05539.1"
                     /translation="MSIIVGSETKLAVSGLTGREGSFHGLRNKAYGTDLVAGVTPGKG
                     GQDVEGVPVFDTIKDAVTEAGANTSMIFVPARFAAPAIDEAIESGVETVIAITEGIPV
                     LDMLKTYWKAKEAGVQLIGPNCPGVLSPGKANVGIIPAQFFDPGPIGVVSKSGTLTYQ
                     IGNELKQAGTGNSSIVGIGGDPIVGSDFIDILSLYEADPETKMIVMVGEIGGDAEERA
                     AEFIAAEVSKPVVAYIAGFTAPPGKQMGHAGAIISGSSGTAAAKKEALEAKGVRVGES
                     PTETAAIAVEVLNSL"
     gene            complement(2873..4036)
                     /gene="sucC"
                     /locus_tag="VFB52_04075"
     CDS             complement(2873..4036)
                     /gene="sucC"
                     /locus_tag="VFB52_04075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZO05540.1"
                     /translation="MDLLEYQGKQLFAKHGIAVPSGEVADTVEEAVAAAEKIGYPCAI
                     KAQVLIGGRGKAGGIKIAQNADEAREYADAIIGMDITGPHGEGPFRVDQVWIEGGSDI
                     AEEYYASVILDRGEKKLLAMVSAKGGMDIEAVAAEDPDALVKVHIDPTSEFDAAAARP
                     IVEAAGLAADALDEAADVLAKLAAVAAGEDATLIEVNPLIVTAGRDVVALDAKVTIDG
                     NSLFRHEDLAAVADTVDDPQEQMAKEKGLTYVKLDGNVGILANGAGLCMSTLDVVAQA
                     GGAPANFLDAGGGSKAEAIVNALEVIKSDAKVTAILFNIFGGITRCDEIAKGIVTASQ
                     QIGLELPLVVRLDGTNSEEGLKILAEANLPNLHQEKTMLDSARRVVELAGGAA"
     gene            4186..4869
                     /locus_tag="VFB52_04080"
     CDS             4186..4869
                     /locus_tag="VFB52_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZO05541.1"
                     /translation="MALPSLKNINWVTTDCYGTLIDWEKGIIDACKKEASKDGFSFEE
                     GPFLERFMEVQAEIMAGSYELYAEVLRRTIVKVAGELGWEVEPSRAQFLPNSVAYWLP
                     FREANAAMDRLGKKYEIGIISNIDDKLLGVSRRHLRTELDLVVTAQQVRSYKPDPVHF
                     KECARRIGGKKGWLHIGCDYTTDVAPLLKMNVPVIWVNRHGEKLEGRKAPAATVKNFR
                     DAAKKLGAG"
     gene            4999..6807
                     /locus_tag="VFB52_04085"
     CDS             4999..6807
                     /locus_tag="VFB52_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZO05542.1"
                     /translation="MGLRLLFAASLLALFCALAFAAAQGADAAPKKGKTKNTLLPEGQ
                     KPNFVVIQTDDQTIDSLYATFTPPGGVPIQVMPNTLTLIGAKGVTLTRYYVSYPLCCP
                     SRVSLLTGRYAHNHNVRGNVQPNGGYLGFSFRGANTNNIATWLQGAGYRTIHIGKFLN
                     GYGDEPYDNGSTVPPGWNAWHTVLKADTNHYFYGYELNNNGNVEGPYGDSGSWETREY
                     SEESRDDFGCPLAPLNGRPCLYETDVFNRIASEEMLGTPGGTPFYLQLDYTAPHGDFR
                     RPAGPEPATRHYNTFLGARLPHSRQQGVNEGNVADKPRFIREAPFLSPTELHTYRVYY
                     EKQLESLRSIDEGVKQVVDTLGAMGQLRNTYIIFTSDNGFFFGEHRLVGGKFLAYEPA
                     THLPFLMRGPGIKPATTTGELAMNIDIAPTILELAGVTPTKSVDGRSLVPYVTDTSLR
                     TRRPLLFESFVETNDVEANGAEPSATKSGAATSSNSEATASITAPPKDYEGIRYGAYK
                     YIAWPSGEKELYDITKDPFELNNLARDKNYAPIRNFLHNHLRILEDCSGNTCREPLPK
                     LPLTKREQLKVKRQKEKEQREREQRRKERESKKGSK"
     gene            complement(6826..>7550)
                     /locus_tag="VFB52_04090"
     CDS             complement(6826..>7550)
                     /locus_tag="VFB52_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZO05543.1"
                     /translation="APGPIGSSYREPSGPGVRVDSGVEGGFEVTPMYDPMVAKLIVWD
                     VDREKATRRMLRALSEYDSGGLTTLIPFHEAILATEQWDKGETCRDLMEDNDWLKSTA
                     PADASPPEPSEDGGEIVTRDYKVEVSGKLFDVKVIGEAVAGTAAAAPAPGGKKPPKRE
                     RKAGGGASSSSEALPAPLQGTVFKVVVEEGAEVAEGDLICVIEAMKMENEITAHRAGK
                     VTSLSIKEGDAVNSGDTLATIE"
BASE COUNT         1223 a         2703 c         2455 g         1169 t
ORIGIN      
        1 tgggcgaacg cacgcgcctc tttcactgga gctacccggg gctgttcgac gaggaacggg
       61 cgaccttcct ggtctggcgc cagggcggcg cggtcgccgc ggtcttcgcc gccggcggtg
      121 acggcgccgc caacgacgcc accgcgctcg cgctcgccca gcgccagcag caccacctcg
      181 aagaacccga gcccttctcg ccggctgaat tcgacgacac cgaagtcccg ctcgaaaacc
      241 ctgcaatcga gatcccgatc tactggctgg gccgtgacct cgccgcccgc tccggcctgc
      301 tcgccctgca cctgcagaac accagctcct cttcgccggc ggaaaccggg cgccctcgcg
      361 ccgggaccta ctacgaagac cgcccgctgc gcgctcgcgg ggaaggcgtc tacgtggacg
      421 tctactcggc cgcgcagtgg aagcgcttca aggcgcgcgg gcacctcccg ggcgcgcttg
      481 agtgcggcgc caccgcgcac ccgatcgagc tgccgcaggg gcacgccgtc gccttccacg
      541 gtcgcgcccg gctgttcagg cggtgcgacc ggcgcgaacc cgagccaacc gagtgggtgc
      601 tgcgggctcg cgtcggcggg gtcgtcgtca ccgcctcgac gatcgagagc tgcgccacct
      661 gcgccgaacc gggccgcggc ccctacaact cctacaaggg catgcgcgcg atcgcccgcg
      721 gcctcgagct gaggtcgcgc taggtgggaa actcgaccgg gttccggatc ggcggcatcg
      781 cggtcctctg tgtagccgcg gcgctggtcg tcgccttggt cgtcaccgac acctccggcg
      841 acgccgccta catgccgccg aacgtcgacc agacggagat ggtcctgcgc ctttccgacc
      901 tcccgccggg ctacgtcaac ggctacctcg gcgagggctt cgacgacggc gatccgcgct
      961 gctcggcggt gaaagacccg ccgggcgcgc cggcgcggct ctccgagttc gtcgaggcct
     1021 accacccgag cggctgcgtc gcggcttacg ccgcgcgctg gacctttccc ggcgagccgt
     1081 cggtcgcgtc cacggtcgcc agcggcgtgc tggctctcgg cagcgcggag gcggtcgatg
     1141 cggcctggga ggcggcgccg accgtgctgc tcggccgcgt cttcccgagg agcgagccga
     1201 ccgaggtgga gccgcaggcc atggtcgggg tacggacgcg gctgttccac acccgggcca
     1261 ccctccccta ctcctacggc ggcaacgaca aggcttcctt cctgatctgg cagacgggca
     1321 acacgatggc cacggtgatg gcggtcggca ggtcgtacgc gacgaccgac cgagtggccg
     1381 ccgaactggc gccgcgccag acgaagcgca tccgcaagcc gacccccttc aggcagtccg
     1441 aacgctacga cggcgaagtc ggcctcgacg acccgcagat cgacgtcccc gtctactggc
     1501 tcggccgcac cttcaagcca ggcaatggaa tgccgccgaa cgagctgttc gagtcctact
     1561 ggcggggcaa accggaagcg gaaaagagcg ggtccgggcg cttcttctcg ccgcaggcgc
     1621 ccgccgggcc gctgacgatc aggtactaca acttccgcct cgacacctgg acgccgtcga
     1681 cctggagggt cttcgccggc tcgaagtccg cccgcgtgct caccacctgg gagtgcaccg
     1741 agatccgcac gatcaagctg cccgggggga gcgcgacgat cttcggcggc tacgccaggg
     1801 acttcaagga ctgcccgagc aagccgccga gcgtgttcac cgcctgggtc aacctcggcg
     1861 gcgtcaaggt cgtcgtcaac ccacccgaat cgcccgacgg tggcctcttc agcaccccct
     1921 acagctcctt cgccggcatg gaagcgatcg cccgcggcct gaagctgcgc gagcagccgc
     1981 tgttctaggc gcgcggcgcc ctagagcgag ttgaggactt cgaccgcgat cgccgcggtc
     2041 tcggtcgggc tttcgccgac ccgcacgccc ttcgcctcga gcgcctcttt cttcgccgcg
     2101 gcggtgccgg aggagccgga gatgatcgcg ccggcgtggc ccatctgctt gccgggcggc
     2161 gcggtgaagc cggcgatgta ggcgaccacg ggcttggaga cctcggcggc gatgaactcg
     2221 gcggcgcgct cctcggcgtc gccgccgatc tcgcccacca tcacgatcat cttcgtctcg
     2281 gggtcggcct cgtagaggct gaggatgtcg atgaagtcgg agccgacgat cgggtcgccg
     2341 ccgatcccga cgatcgagga gttgccggtc ccggcctgtt tcagctcgtt gccgatctgg
     2401 taggtcagcg tgccgctctt ggagacgacg ccgatcgggc ccgggtcgaa gaactgggcg
     2461 gggatgatgc cgacgttggc cttgccgggc gagagcacgc cggggcagtt cggcccgatc
     2521 agctggaccc cggcctcctt ggccttccag taggtcttca gcatgtcgag caccgggatg
     2581 ccctcggtga tcgcgatcac ggtctcgacc ccggactcga tcgcctcgtc gatcgccggg
     2641 gcggcgaaac gggcggggac gaagatcatc gaagtgttgg cgccggcctc ggtgaccgcg
     2701 tccttgatcg tgtcgaagac cggcacgccc tcgacgtcct gaccgccctt gccgggggtg
     2761 acgccggcga ccaggtcggt cccgtaggcc ttgttgcgca gaccgtggaa ggagccctcg
     2821 cgcccggtca gcccggagac ggcgagcttc gtctcgctgc cgacgatgat gctcatgcgg
     2881 cacctccggc gagctcgacg acccggcgcg cggagtccag catcgtcttc tcctggtgca
     2941 ggttcggcag gttggcctcg gcgaggatct tcaggccctc ctccgagttg gtgccgtcga
     3001 gccggacgac cagcggcagt tccagcccga tctgctgcga ggcggtgacg atgcccttgg
     3061 cgatctcgtc gcagcgggtg atcccgccga agatgttgaa gaggatcgcg gtcaccttcg
     3121 cgtcggactt gatgacttca agcgcgttga cgatcgcctc ggctttcgag ccgccgccgg
     3181 cgtcgaggaa gttggcgggg gcgccaccgg cctgcgcgac cacgtcgagg gtcgacatgc
     3241 agagcccggc gccgttggcg aggatgccga cgttgccgtc gagtttcacg taggtgaggc
     3301 ctttttcctt cgccatctgc tcctgggggt cgtcgacggt gtcggcgacc gcggcaaggt
     3361 cctcgtggcg gaagagcgag ttgccgtcga tcgtcacctt ggcgtcgagc gcgaccacgt
     3421 cgcggccggc ggtgacgatc agcgggttga cctcgatcag ggtcgcgtcc tcgccggcgg
     3481 cgaccgcggc cagcttggcc agcacgtcgg cggcctcgtc gagcgcgtcg gcggccagcc
     3541 cggcggcctc gacgatcggc cgcgccgcgg cggcgtcgaa ctcggaagtc gggtctatgt
     3601 ggactttgac cagcgcgtcg gggtcctcgg cggcgaccgc ctcgatgtcc atcccgcctt
     3661 tcgcggagac catcgccagc agcttcttct cgccgcggtc gaggatcacc gaggcgtagt
     3721 actcctcggc gatgtccgag ccgccctcga tccagacctg gtcgacgcgg aacgggccct
     3781 caccgtgcgg gccggtgatg tccatgccga tgatcgcgtc ggcgtactcg cgcgcctcgt
     3841 cggcgttctg ggcgatcttg atcccgccgg ccttgccgcg accgccgatc agcacctggg
     3901 ccttgatcgc gcaggggtag ccgattttct cggcggcggc gaccgcctcc tcgaccgtgt
     3961 cggcgacctc accgctgggc acggcgatgc cgtgtttggc gaaaagctgc tttccctggt
     4021 actcgaggag gtccataaat aggtggcctg gtggggcggc gggcacccta tatgaagtca
     4081 gtcgcgggcc gccgcgacgc acgctgagtg gtaggggaaa ccgctaagcg gtgccccgat
     4141 ggcataatgc cgcccccgcc cggaactccg ggcgttctca cgcttatggc gctaccaagt
     4201 ctcaaaaaca tcaactgggt gaccaccgac tgttacggca ccctgatcga ctgggagaaa
     4261 ggcatcatcg acgcctgcaa gaaagaggcg tccaaagatg gcttctcgtt tgaggagggc
     4321 ccgttcctgg aacggttcat ggaggtccag gccgagatca tggccggctc ctacgagctc
     4381 tacgccgagg tcctgcgtcg cacgatcgtc aaagtcgccg gcgagctcgg ctgggaggtg
     4441 gagccctcgc gggcccagtt cctgcccaac agcgtcgcct actggctgcc cttccgcgag
     4501 gccaacgcgg cgatggaccg gctcgggaaa aagtacgaga tcgggatcat ctccaacatc
     4561 gacgacaaac tgctcggggt ctcgcggcgc cacctgcgca ccgaactcga cctcgtcgtc
     4621 accgcccagc aggtgcgcag ctacaagccg gacccggtcc acttcaaaga gtgcgcgcgc
     4681 cgcatcggcg gcaaaaaagg ctggctccac atcggctgcg actacacgac cgacgtcgcc
     4741 ccgctgctga aaatgaacgt cccggtgatc tgggtcaacc gccacggcga gaaactcgag
     4801 gggcgcaaag cccctgctgc gacggttaaa aacttccgcg acgccgcgaa aaaactcggc
     4861 gccggctgag caccccgtcc tcttcgtttt ttaccgttca aggcgcaact ccgggaggag
     4921 gcgccggttc ttcgtcgtag tcggccagaa cgccctatga ataggttcga tctccccagc
     4981 acccaatcgc gtgccctcat gggtctgcgc ctgctgttcg cggccagcct gctggcgctg
     5041 ttctgcgcgc tcgccttcgc cgccgcccag ggtgcggacg ccgccccgaa gaagggcaag
     5101 acgaagaaca cgctgctgcc ggaagggcag aaaccgaact tcgtcgtgat ccagactgac
     5161 gatcagacga tcgacagcct ctacgcgacc ttcacgcccc cgggcggggt gccgatccag
     5221 gtgatgccga acacgctgac gctgatcggc gccaaaggcg tgaccctgac ccggtactac
     5281 gtctcctacc ccctctgctg cccctcccgg gtcagcctgc tgaccggccg ctacgcgcac
     5341 aaccacaacg tgcgcggcaa cgtccagccc aacggcggct acctcggctt ctccttccgc
     5401 ggcgccaaca ccaacaacat cgcgacctgg ctgcaggggg ccggctaccg cacgatccac
     5461 atcggcaagt tcttgaacgg ctacggcgac gagccctacg acaacggctc gaccgtgccg
     5521 ccgggctgga acgcctggca cacggtcctg aaggccgaca ccaaccacta cttctacggc
     5581 tacgagctca acaacaacgg caacgtcgaa ggcccttacg gcgactccgg gagctgggag
     5641 acgcgcgaat acagcgaaga atcgcgcgac gacttcggct gcccgctggc gccgctgaac
     5701 gggcgcccct gcctctatga gaccgacgtc ttcaaccgga tcgcgagcga agagatgctc
     5761 ggcacgccgg ggggcacgcc gttctacctg cagctcgact acacggcccc gcacggcgac
     5821 ttccgccgcc cggccgggcc ggagccggcg acccgccact acaacacctt cctcggcgcc
     5881 cggctcccgc acagccgcca gcagggggtc aacgagggca acgtcgccga caagccgcgc
     5941 ttcatccgcg aagcgccgtt cctctcgccg accgaactgc acacctaccg cgtctactac
     6001 gagaagcagc tggagtcgct gcgctcgatc gacgaaggcg tcaaacaggt ggttgacacg
     6061 ctcggcgcga tgggccagct gcgcaacacc tacatcatct tcacctccga caacggcttc
     6121 ttcttcggcg agcaccgcct ggtcggcggc aagttcctcg cctacgagcc ggccacccac
     6181 ctgcccttcc tgatgcgcgg accggggatc aagccggcga ccacgaccgg cgagctggcg
     6241 atgaacatcg acatcgcccc gacgatcctc gagctggccg gcgtcacccc gaccaagagc
     6301 gtcgacggcc gctccctggt cccctacgtc accgacacca gcctgcgcac ccgccgcccc
     6361 ctgctcttcg agtccttcgt cgagaccaac gacgtcgagg ccaacggcgc cgaaccgtcg
     6421 gcgaccaaat caggcgccgc cacctccagc aactccgagg cgaccgcctc gatcaccgcg
     6481 ccgccgaagg actacgaggg gatccgctac ggggcctaca agtacatcgc ctggccctcc
     6541 ggcgagaaag agctctacga catcaccaaa gacccctttg agctcaacaa tctcgcccgc
     6601 gacaagaact acgccccgat ccgcaacttc ctccacaacc acctgcgcat cctcgaggac
     6661 tgctccggca acacctgccg cgaacccctc ccgaagctgc cgctaaccaa gcgcgagcag
     6721 ctgaaggtca aacggcagaa ggaaaaggaa cagcgcgaac gcgaacagcg ccgcaaagaa
     6781 cgcgaatcga aaaaagggtc gaaatagcgc cgtaggcgcg gcgcgctact cgatcgtggc
     6841 gagggtgtcg ccggagttca ccgcgtcgcc ctctttgatg ctcagtgagg tgaccttgcc
     6901 ggcgcggtgg gcggtgatct cgttctccat cttcatcgcc tcgatcacgc agatcaggtc
     6961 gccctcggca acctcggcgc cctcctcgac gacaactttg aagacggtgc cctgcagcgg
     7021 ggcggggagc gcctcggagg aggagctggc gccaccgcct gccttgcgct cgcgtttcgg
     7081 cggtttcttg ccccccggtg cgggcgccgc ggcggcggtc ccggcgacgg cctcgccgat
     7141 caccttgacg tcgaagagct tgcccgagac ctcgaccttg tagtcgcggg tgacgatctc
     7201 gccgccgtcc tcgctcggct ccggcgggga tgcgtcggcg ggggcggtcg acttgagcca
     7261 gtcgttgtcc tccatcaggt cgcggcaggt ctcgcccttg tcccactgct cggtggcgag
     7321 gatcgcttcg tggaagggga tcagggtggt gaggccgccg gagtcgtatt cgctgagggc
     7381 gcgcaacatg cggcgcgtcg ccttctcgcg gtcgacgtcc cagacgatca gcttggccac
     7441 catcgggtcg tacatcggcg tcacctcgaa gccgccctcg accccggagt cgacgcgcac
     7501 gcccggaccg gagggctcgc ggtaggagct gccgatcggc cccggcgccg
//
LOCUS       DASRIG010000149         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2687699, whole genome shotgun sequence.
ACCESSION   DASRIG010000149 DASRIG010000000
VERSION     DASRIG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687699"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFB52_04095"
     CDS             complement(<1..652)
                     /locus_tag="VFB52_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05544.1"
                     /translation="MSEPPAGAATKASAASAPNRDWGSTAANIAGKYGLLITFFLTIA
                     VFCILRPDTFATWDNAESILTLAAPPMIMAVGLTVVLVMQDFDLSFGAMIGLAGGAVT
                     TFIVSDGMGWGLAILLVLGIGVLVGILNGFMVAYLGGNSFIITLAMGTVLTGVEFALT
                     GQDTVFQGLPLDFLDIASGSSILGFNNQIWIAVGVIIIAWLLMDRTELGRYMYAIGG"
     gene            complement(796..2985)
                     /locus_tag="VFB52_04100"
     CDS             complement(796..2985)
                     /locus_tag="VFB52_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO05545.1"
                     /translation="MSTPDYDVIAAEVHRKAMQNITDEMAITLVRTSGSPIVTESKDF
                     STCLMNTVPEHLGFSSYVLFHVGSSLIGTQVIANEAAALTDLKPGDGWVVNDPHSAGA
                     MHQGDVSIIMPTFYGDEHIGWSFANMHVLDVGGVGISGYAPGAHDVWQEGMLFPPVRI
                     IRDGKIDTEWEKYIAANVRAPGPVLNDIRSMIASNNTASKKLHGVIDEFGLEAHREFC
                     AINMDLSEQVMRERIEKIPDGTYEAVDWNEFDGHEGPDQLLELRLKLEVDGSDLRYFY
                     TGVPQIDAFVNSTEGPMFGQAMTGLMTMMLYGDLPVNGGLWRPISVDIGEPGTIVNAV
                     PPAPVSNAHSEVGMRACKLSKDVLCQAFSLSEDPVLRSRIAGQHQDGFPGNALFGNNQ
                     HGGVSVVFYPDNAIGSGGGAQTINDGQDAYGLTCTTGGGIPDIENHEGADPVLFLWRR
                     LIPNSGGPGQKRGGQSMDQAYAVRYSDLMAGPGFNACAKVPPHGVGGGYPASAGDFYP
                     VRETNIDELLSKSELPVMERFEGKMEEVRSKLTHLKLGRDDVFVALSGGGGGIGDPLL
                     REAELVAQDVVGGYITADHAINVYGVISDGEGGVDAAATEAKREEIRKARIGGDPSKP
                     LSEPPSIGISLASQDGSWSCTSCEQELASNGGNWREGAVLKETLITDRYAEMEMKVRG
                     RTEGAKVVTREHFCPSCAMSLGVDVATEDLETLAAPDVRSAAAAAAS"
     gene            complement(3004..>4872)
                     /locus_tag="VFB52_04105"
     CDS             complement(3004..>4872)
                     /locus_tag="VFB52_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO05546.1"
                     /translation="TTVVTNTITTLRRSRVGVLITSGFKDAFRLAGGPRTTEIDDHLQ
                     LNVPDLMDRRAIAEIDERTDWSGSVLVPVDIEGVKAAAKHLVEDVGIDALAICFLWSH
                     ANAENELAAEAAVKELYPDLFVTTSQSVFPVEGETRRWTTAVLNSFVQDKADVYLTSL
                     NTKLRDAGLKGGLAFFQGLGGGISLEKARQYPLGLLGSGPAGGAIGANELAKQMGFKR
                     VLLGDMGGTSFDTGIISDNEIHIDKNLQLGPFMTGVNIVDVISVGAGGGSIGWVSERG
                     VPQVGPQSAGSTPGPAALGRGGTEPTVTDAMVTMGFIDPDHYLDGRVQLKPELAREAL
                     DSQLGSRFGWSTEEAAAAVHDLVVVNMANAVREVSVGKGHDPREFLFLSYGGTLPLFS
                     SQIAERLGISTIVIPRNSSVFCALGLLSSDFILRTDQGVGWDLSKPEGVGRVNEIAEE
                     MVKTAKQEMLDEGFSEDQIEIQRTADIRFHGQAYELTLPMPARTLTEDDAPALFDQFL
                     EFYERTYGEGTAWKGVPASMVNYSVTVVGRQPRPDVDTAASEPAKPEDIERERRTVFL
                     PAERRHEEIPVYDDLRFTAGTAIEGPAIIDAADTTIYVPPGTKAERDKYMNYVLTR"
BASE COUNT          729 a         1567 c         1749 g          827 t
ORIGIN      
        1 tgccgccgat cgcgtacatg tagcggccga gctcggtgcg gtccatcagc agccaggcga
       61 tgatgatcac cccgaccgcg atccagatct ggttgttgaa gccgaggatc gagctgccgc
      121 tggcgatgtc gaggaagtcg agcgggaggc cctggaagac cgtgtcctgt ccggtcagcg
      181 cgaactcgac gccggtcagg accgtgccca tcgcgagcgt gatgatgaac gagttgccgc
      241 cgaggtaggc gaccatgaag ccgttgagga tcccgaccag gacgccgatc ccgaggacca
      301 ggaggatcgc caacccccag cccatcccgt cggagacgat gaaggtggtg accgcgccgc
      361 cggcgaggcc gatcatcgcc ccgaaggaga ggtcgaagtc ctgcatcacc aagaccacgg
      421 tcagcccgac ggccatgatc atcggcggcg ccgccagggt gaggatcgat tccgcgttgt
      481 cccaggtcgc gaaggtgtcg ggccgcagaa tgcagaagac cgcgatcgtc aagaagaagg
      541 tgatcagcag cccgtacttg ccggcgatgt tggccgccgt cgaaccccag tcccgattcg
      601 gggcactggc cgccgaagcc ttcgtcgcag ccccggcggg gggctcactc acagtctgat
      661 tctcacccac ggatcccatc cctcgttcgc gttgtcagta gtgactgagg ccgaaaaggc
      721 cacccgtagc caggctccca gggggggagc cggggggcta ctagcggatg cgctagaagt
      781 ggctcgggaa gcggctcagc tggccgccgc ggcggccgcg ctgcgcacgt caggagcagc
      841 cagcgtctcg agatcctcgg tggcgacgtc gacgccgagg ctcatcgcgc agctcgggca
      901 gaagtgctcc ctggtgacga ccttggcgcc ttcggtgcgc ccgcggactt tcatttccat
      961 ctccgcgtaa cggtcggtga tcagggtctc cttcaagacc gcgccctcgc gccagttgcc
     1021 accgttgctc gcgagctcct gctcgcagga ggtgcacgac caggagccgt cctgactggc
     1081 gagcgagatg ccgatgctcg gcggctcgct gagcggcttg ctcgggtcgc cgccgatccg
     1141 tgccttgcga atctcctcgc gcttggcctc ggtcgcggcg gcgtcgacgc cgccctcgcc
     1201 gtcggagatc acgccgtaga cgttgatcgc gtggtcggcc gtgatgtatc cgccgaccac
     1261 gtcctgggcg accagctcgg cctcgcgcag gagcggatcg ccgatcccgc cgccgccacc
     1321 ggagagcgcg acgaagacgt cgtcacgacc cagtttgagg tgggtgagct tcgagcggac
     1381 ctcttccatc ttgccctcga agcgctccat caccggcagc tcgctcttcg agagcagctc
     1441 gtcgatgttg gtctcgcgga ccgggtagaa gtcaccggcg ctggccgggt agccgccgcc
     1501 gacaccgtgg ggcgggacct tggcgcaggc gttgaagccc gggccggcca tcaggtcgga
     1561 gtaacggacc gcgtaggcct ggtccatcga ctggccgccg cgcttctggc cggggccgcc
     1621 cgagttcggg atcagccgcc gccagaggaa cagcaccggg tccgcgccct cgtggttctc
     1681 gatgtcgggg atgccgccac cggtggtgca ggtgaggccg taggcgtcct gaccgtcgtt
     1741 gatcgtctgg gcgccgccgc cggagccgat cgcgttgtcc gggtagaaga ccaccgagac
     1801 gccgccgtgc tggttgttgc cgaacagggc gttgccgggg aagccgtcct ggtgctgacc
     1861 ggcgatgcgg ctgcgcagga ccgggtcctc gctcagcgag aaggcctggc agagcacgtc
     1921 cttggagagc ttgcaggcgc gcatcccgac ctcggagtgg gcgttggaga cgggcgccgg
     1981 cgggaccgcg ttgacgatcg tgccgggctc gccgatgtcg accgagatcg gccgccagag
     2041 accgccgttc acgggcaggt cgccgtagag catcatcgtc atcaggccgg tcatcgcctg
     2101 gccgaacatc gggccctcgg tcgagttgac gaaggcgtcg atctgcggca cgccggtgta
     2161 gaagtagcgc aggtcggagc cgtcgacttc gagtttgagc cgcagctcga gcagctggtc
     2221 ggggccctcg tggccgtcga actcgttcca gtcgaccgct tcgtaggtgc cgtccgggat
     2281 cttctcgatc cgttcgcgca tcacctgctc ggagaggtcc atgttgatcg cgcagaactc
     2341 acggtgagcc tcgaggccga attcgtcgat cacgccgtgg agctttttcg aagcggtgtt
     2401 gttcgaggcg atcatcgagc ggatgtcgtt gaggaccggc cccggggcgc gcacgttggc
     2461 ggcgatgtac ttctcccact cggtgtcgat cttgccgtcg cggatgatcc gcaccggcgg
     2521 gaacagcatc ccctcctgcc agacgtcgtg ggcgcccggc gcgtagccgg agatgccgac
     2581 gccgccgacg tcgaggacgt gcatgttggc gaaggaccag ccgatgtgct cgtcgccgta
     2641 gaaggtcggc atgatgatcg agacgtcgcc ctggtgcatc gcgccggccg agtgcgggtc
     2701 gttgacgacc cagccgtcgc cgggcttcag gtcggtcagg gcggccgctt cgttggcgat
     2761 cacctgcgtg ccgatcagcg aggagccgac gtgaaacagc acgtaggagg agaacccgag
     2821 atgctcgggg accgtgttca tcaggcaggt cgagaagtcc ttcgactcgg tgacgatcgg
     2881 cgacccggag gtgcgaacca gggtgatcgc catctcgtcg gtgatgttct gcatcgcctt
     2941 gcgatgcacc tcggccgcga tgacgtcgta atcgggggtg ctcatggctc tggttcctct
     3001 cctctagcgg gtcaggacgt agttcatgta cttgtcgcgc tccgccttgg tccccggcgg
     3061 gacgtagatc gtggtgtcgg cggcgtcgat gatcgccggc ccctcgatcg cggtgccggc
     3121 ggtgaaccgg aggtcgtcgt agacgggaat ctcctcgtga cgacgctcgg cggggaggaa
     3181 gacggtgcgg cgctcgcgct cgatgtcctc gggcttggcc ggctcgctcg ccgcggtgtc
     3241 gacgtcgggc cgcggctgac gcccgaccac ggtgacgctg tagttgacca tcgaggcggg
     3301 aacgcccttc caggcggttc cttcaccgta cgttctctcg tagaactcga ggaactggtc
     3361 gaacagcgcc ggagcgtcgt cctcggtcag cgtccgcgcc ggcatcggca gggtgagctc
     3421 gtaggcctgg ccgtggaagc ggatgtcggc ggtgcgctgg atctcgatct ggtcctcgga
     3481 gaagccctcg tcgagcattt cctgcttcgc cgtcttgacc atctcctcgg cgatctcgtt
     3541 gacccggccg acgccctcgg gcttgctcag gtcccagccg acgccctggt cggtgcgcag
     3601 gatgaagtcc gaggagagca gcccgagcgc gcagaagacg gagctgttgc gcgggatcac
     3661 gatcgtcgag atccccagcc gctcggcgat ctggctggag aacagcggca gggtgccgcc
     3721 gtaggagagg aagaggaact cgcgcgggtc gtggcccttg ccgacgctga cctcgcggac
     3781 cgcgttggcc atgttgacga cgaccaggtc gtggaccgcg gccgcggcct cctcggtgct
     3841 ccacccgaac cgcgagccga gctggctgtc gagcgcctcg cgggcaagct ccggtttcag
     3901 ctgcacgcgg ccgtcgaggt agtggtcggg gtcgatgaag cccatcgtca ccatcgcgtc
     3961 ggtgacggtc ggttcggtgc cgccgcgccc gagcgccgcc gggccggggg tcgagccggc
     4021 gctctgcggg ccgacctggg gcacgccgcg ctcggagacc cagccgatcg agccgccgcc
     4081 ggcgccgacc gagatcacgt cgacgatgtt gacgccggtc atgaacgggc cgagctggag
     4141 gttcttgtcg atgtggatct cgttgtcgga gatgatcccg gtatcgaagc tggtcccgcc
     4201 catgtcgccg agcaggaccc gtttgaagcc catctgcttg gccagctcgt tggcgccgat
     4261 cgcgccgccg gccgggccgg agccgagcag cccgagcggg tactggcggg ctttttcgag
     4321 gctgatgccg cccccgaggc cctggaagaa ggcgaggccg cccttgaggc cggcgtcgcg
     4381 cagtttcgtg ttcagcgagg tcaggtagac gtcggctttg tcctgcacga aggagttgag
     4441 gacggccgta gtccagcgcc gggtctcgcc ctcgaccggg aagacgctct gggaggtggt
     4501 gacgaagagg tccgggtaga gctccttgac cgcggcttcg gcggcgagct cgttctcggc
     4561 gttggcgtgg ctccagagga agcagatcgc gagggcgtcg atgccgacgt cctcgaccag
     4621 gtgcttggcc gcggccttga cgccctcgat gtcgaccggc acgagcacgc tgcccgacca
     4681 gtcggtccgc tcgtcgatct cggcgatcgc gcggcggtcc atcaggtcgg gcacgttgag
     4741 ctgcaggtgg tcgtcgatct cggtcgtgcg cggaccgccg gcgaggcgga aggcgtcctt
     4801 gaagccggag gtgatcagca cgccgacgcg cgagcggcgc agggtggtga tcgtgttggt
     4861 gacgacggtg gt
//
LOCUS       DASRIG010000150         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1881029, whole genome shotgun sequence.
ACCESSION   DASRIG010000150 DASRIG010000000
VERSION     DASRIG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881029"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..560)
                     /locus_tag="VFB52_04110"
     CDS             complement(120..560)
                     /locus_tag="VFB52_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO05547.1"
                     /translation="MAESEELLDEKEAIKRLNVALELQYRSVLQYSVVAASLTGMQVL
                     ALGPQLTEYGDRELSGARTLIEKIVSFGGEPTTEVAALKHETDPEKALAWLVDCEQEA
                     IEALQAAIEPTGREGRSEALEHMLEHQIMRKQEQADFLARALAA"
     gene            724..1539
                     /locus_tag="VFB52_04115"
     CDS             724..1539
                     /locus_tag="VFB52_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05548.1"
                     /translation="MALSVLVVLLAVFAWPQSDDGEGGGPLNAIAAAAERTQSEPGMR
                     GTMRAVVTSPNPDESITMTGRSVFDETKERSRMLMKFPVPALDEVVEMEMVGDGTMMF
                     MRSDMFGDELPDGREWMGMDLSFGGELDLPVPAANDAKGELELLEAATGGVRKLGREK
                     VRGVATTHYSGTVALDEQVERLREEGADEIASFVEDEGGPMEIEVWIDADGLVRRMQV
                     VQTQPAEDGVGSVSVEMEMDFFDFGPVPAIEIPESDEVFDITSLAKEQISGGT"
     gene            complement(1549..>2428)
                     /locus_tag="VFB52_04120"
     CDS             complement(1549..>2428)
                     /locus_tag="VFB52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05549.1"
                     /translation="ELLRGAISLTYGIYQLTQVIGPLIGGLMIAAFGIGVPYAVDAAS
                     CLGMAFAAAAMSPQPVHSEVEHEPVLRAIRSGISFVRRLKALMGSFVIDLAAMTFGMP
                     RALFPVLAVGVFDAGAAGTGVLFAAVAAGSTVAALTTGWLVHARFLGRITLAAVAVWG
                     VCIALAGFMESLWPAAALFALAGAADSVSAVCRTTISQTLTPDRMRGRMSSVFSLVVA
                     GGPRLGDVESGTVAALTSPAFSVVSGGLACLASVGVVAALYPQLAAYDGDKVGAGEVG
                     QDKPARGGPGDDPVGL"
BASE COUNT          389 a          776 c          927 g          336 t
ORIGIN      
        1 cgcggaagga tcgcgcctgc gcggtttgca ccggcgcgcg ggcagcgtcc tggcgacgcc
       61 ggtgatgcgc gccaaggagc ggcgctggct cgccgccgcc tgaccgcgac cgccgcccgc
      121 taagccgcga gcgcccgcgc caggaagtcg gcctgctcct gcttgcgcat gatctgatgc
      181 tcgagcatgt gctcgagggc ctcggaacgg ccctcacggc cggtcggctc gatcgcggcc
      241 tgcagggcct cgatcgcttc ctgctcgcag tcgaccagcc acgccagtgc cttctccgga
      301 tcggtctcgt gcttcagcgc ggcgacctcg gtggtcggct cgccgccgaa ggagacgatc
      361 ttttcgatca gcgtccgggc gccactcagc tcgcggtcgc cgtactcggt cagctgcggg
      421 ccgagagcca gcacctgcat cccggtcagg ctggcggcca cgacggagta ctgcaggacc
      481 gatcgatact ggagctcgag ggcgacgttg aggcgtttga tcgcctcctt ctcgtcaagc
      541 agctcttccg actcggccat ggtcctctcg ccgtaccctc cgccggcggg ttgaatcaga
      601 gggtgaggcg ccaaccgata ccgaggcggt acctttcaaa gcgctctggg ggacgcgatc
      661 acatctgagc tgggccacgg cgggtcaccg aagcgcggcc gccggggtct ctggctgacc
      721 gtggtggcgc tttcggtctt ggtcgtcttg ctcgccgtgt tcgcctggcc gcagagcgac
      781 gacggcgagg gcggcgggcc gctgaacgcg atcgccgcgg cggcggagcg gacgcagagc
      841 gaaccgggca tgcgtggcac gatgcgcgcg gtggtcacct cgcccaatcc ggacgagtcg
      901 atcacgatga ccggccggtc cgtcttcgac gaaacgaagg aacgcagccg gatgttgatg
      961 aagttcccgg ttccggcgct ggacgaagtg gtcgagatgg agatggtcgg cgacggaacg
     1021 atgatgttca tgcgctccga catgtttggc gacgagctgc ccgacggtcg cgaatggatg
     1081 gggatggacc tctccttcgg aggggaactg gacctgcccg tccccgccgc caatgacgcg
     1141 aaaggggaac tcgaactgct cgaagcggcc accggtggcg tccgcaagct gggccgggag
     1201 aaggtgcgcg gcgtggcgac gacgcactac agcggcacgg tcgccctcga cgagcaggtc
     1261 gagcggctgc gcgaggaagg agcggacgag atcgcctcct tcgtcgaaga cgagggcggg
     1321 ccgatggaga tcgaggtgtg gatcgatgcc gacggtctgg ttcgccggat gcaggtagtt
     1381 cagacgcagc cggcggaaga cggcgtggga tccgtgtcgg tggagatgga gatggatttc
     1441 ttcgacttcg gccccgttcc cgcaatcgag atccccgagt ccgatgaagt cttcgacatc
     1501 acctcactcg ccaaggagca gatcagcggc ggcacctagg cgcgttccct atagcccaac
     1561 cgggtcgtcg cccgggccac cgcgcgcggg tttgtcctgg ccgacctcgc cggcgccgac
     1621 cttgtcgccg tcgtaggcgg cgagctgggg gtagagggcg gcgacgacgc cgaccgaggc
     1681 gaggcaggcg aggccgccgg agacgaccga gaaggcgggt gaggtgaggg cggcgacggt
     1741 gccggactcg acgtcgccga ggcgggggcc gccggcgacg acgaggctga agacggagct
     1801 catgcggccg cgcatgcggt cgggggtgag ggtctgggag atcgtggtgc ggcagacggc
     1861 gctgacgctg tcggcggcgc cggcgagggc gaagagcgcg gccgcgggcc agagcgactc
     1921 catgaacccg gcgagcgcga tgcagactcc ccagaccgcg accgcggcga gggtgatgcg
     1981 gccgaggaag cgggcgtgga cgagccagcc ggtggtcagg gcggcaacgg tggagccggc
     2041 ggcgaccgcg gcgaagagca cgccggtgcc ggccgcgccg gcgtcgaaga cgccgaccgc
     2101 gagcaccggg aagagggcgc ggggcatgcc gaaggtcatc gccgcgaggt caatcacgaa
     2161 gctgcccatc agcgccttga ggcggcgcac gaaggagatg ccgctgcgga tcgcgcgcag
     2221 gaccggctcg tgctcgacct ccgagtgcac cggctggggc gacatcgcag cggcggcgaa
     2281 ggccatgccg aggcagctgg cggcgtcgac cgcgtagggc acgccgatcc cgaaggcggc
     2341 gatcatcagg ccgccgatca gcggcccgat cacctgggtg agctggtaga tcccgtaggt
     2401 gagcgagatc gcgccgcgca gcagctcc
//
LOCUS       DASRIG010000151         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3732987, whole genome shotgun sequence.
ACCESSION   DASRIG010000151 DASRIG010000000
VERSION     DASRIG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3732987"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFB52_04125"
     CDS             <1..855
                     /locus_tag="VFB52_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05550.1"
                     /translation="AATDGGDDLTVAGLSGNPAGLPAAAPVGGVITRWTVNSALAIPP
                     EAGYVYGQKLLVLRPLAPTAFQLVGESSFGTILGAVNTFPTRIPVQAGDRLGLGGTFA
                     TLYCETGNPADVMAYDEGTGSPGPVPGGVLTLPDTAAGYQVPAAATIEPDVDGDGYGD
                     ESQDGCPQSAAYQTPCPVVVLDAYSQVGGQAVTVKVATSLASSVTVSGTVKLGKGASA
                     KLSAKAKVVNPGKLGKFTLQFPAALKEKLKEMEPGQKLSLKITAKATNLAGAASTDKL
                     SVKLKGQG"
     gene            893..1828
                     /locus_tag="VFB52_04130"
     CDS             893..1828
                     /locus_tag="VFB52_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05551.1"
                     /translation="MSRRLLTGLVPIAVAASLSFVPAASAATQIGDPCLANYAVDDTT
                     IFQQASPSTGLPAAAPSSGVITKWVVNVSPTSATIPQVLKVVRVLGPTTAQTVAESPQ
                     ENAIAGFNQFAARLPIAAGDRLGLFGAGDAGTLLCDAPTTGADKLGYFPGSPPIGVLS
                     PFAEVAKQRLPVVATIEPDVDADGYGDETQDGCPQSAAVQIPCPAVTVDATSRVGANA
                     ATVLVRTSSDAVVKVIASVKLGKDGKAKLSARAKPVAPGKRVTFALKFPGKLKQKLEE
                     LGPKQKLTLKITASATNVAGQLSTDKLKVKLKGQG"
     gene            2246..2824
                     /locus_tag="VFB52_04135"
     CDS             2246..2824
                     /locus_tag="VFB52_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05552.1"
                     /translation="MSSSKSIPTCFGIGAPGDTLASFNPDVPVGGKAKPFESPDGILL
                     SLAVVIEPDADGDGYGDESQDGCPQSAATQGTCPAPPPVVVTKLVGKPKLEGNVVAVK
                     LTTSAPAKVTVTGSIRGKRAAGPASKTVTPGETGRLYLQLSKATKQRLAKLPRKRKLK
                     MVVEAKVAGAPTVSSEISLPGRKKPQPPRAPR"
     gene            complement(2775..>3623)
                     /locus_tag="VFB52_04140"
     CDS             complement(2775..>3623)
                     /locus_tag="VFB52_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05553.1"
                     /translation="RWLPEPKSLDSRLPDLPGAGLLALGLAALTLAIVEGNDWGWGSP
                     ATLACFAVAALLLLAVVARSRSHPRPIVEPELFAHRSFVVGNVGTILFASAFFSLILG
                     NVLFLTQIWGYTVLQAGAATLPGPAMSTVVAGPAGRLADRFGHRAVIVPGTILFAIGV
                     WVLGTAGAEPDWLGLWLPGALLTGIGIGLAFPTLGAAAVRDVPVDRFATASAVNAAFR
                     QVGAVLGTAILVAIVGDPESLSAALSASDDGYLFAMIAALASGAVALALSAGREAAVV
                     SSAPAS"
BASE COUNT          653 a         1239 c         1262 g          469 t
ORIGIN      
        1 gccgccaccg acggcggcga cgacctcacc gtcgccgggt tgtccggcaa cccggccggc
       61 ctccccgccg cggccccggt cgggggcgtg atcactcgct ggacggtcaa ctcggcgctg
      121 gccatcccgc cggaagcggg ctacgtctac ggtcagaagc tgctcgtgct gcggccgctc
      181 gcccccaccg ccttccagct ggtcggcgaa agcagcttcg gcacgatcct cggcgcggtc
      241 aacaccttcc cgacccggat cccggtccag gccggcgacc gactcgggct cggcgggacc
      301 ttcgcgacgc tgtactgcga aaccggcaac ccagccgacg tgatggcgta cgacgagggg
      361 accggatcac ccggcccggt gccgggcgga gtcctcaccc tccccgacac ggcggccggc
      421 taccaggtac cggccgcggc gacgatcgag cccgacgtcg acggcgacgg ctacggcgac
      481 gagagccagg acggctgccc gcagagcgcc gcgtaccaga ccccctgccc ggtggtcgtg
      541 ctcgacgcgt acagccaggt cggcgggcag gcggtaacgg tcaaagtcgc caccagcctg
      601 gccagctcgg tcacggtgag cggcacggtc aagctcggca aaggtgcgag cgcgaaactc
      661 tcggcgaaag cgaaagtcgt caaccccggc aaactcggca agttcacgct ccagttcccc
      721 gccgccctga aggagaagtt gaaggagatg gagccgggcc agaagctttc gctgaagatc
      781 accgcgaagg cgaccaacct cgccggggcg gcgagcacgg acaagctcag cgtgaagttg
      841 aagggacagg gctagcctcc ggcccagccg cgtggatggg agagtcgaga aaatgagcag
      901 gcgtttgttg acgggcctgg tcccgatcgc agtcgcggcc tcgctctcct tcgtcccagc
      961 cgcctcagcc gcaacccaga tcggtgatcc gtgcctggcc aactacgcgg tcgacgacac
     1021 cacgatcttc cagcaggcat cgccaagcac ggggcttccc gccgccgcgc cgagcagcgg
     1081 cgtaatcacc aagtgggtgg tcaacgtgtc gcccacctcg gcgacgatcc cacaggtgct
     1141 gaaagtcgtt cgcgtgcttg ggccgaccac cgcgcaaacg gtcgcggaaa gcccacagga
     1201 gaacgcgatc gccggcttca accagttcgc cgcccggctg ccgatcgcag ctggcgaccg
     1261 gctcggcctc ttcggcgcag gcgatgcggg caccttgctc tgtgacgcac cgaccactgg
     1321 cgcagacaaa ctggggtact tcccaggctc gccgccgatt ggcgtcctct cccccttcgc
     1381 cgaagtggcc aaacagcgac ttcccgtcgt cgccacgatc gagcccgacg tcgacgccga
     1441 cggctacggc gacgagaccc aggacggctg cccgcagagc gccgccgtcc agatcccctg
     1501 ccccgccgtc accgtggatg cgacctcgcg ggttggcgcg aacgcagcca ccgtcctggt
     1561 caggaccagt tccgacgcgg tggtgaaggt cattgccagc gtgaagctgg gtaaagacgg
     1621 caaagcgaaa ctcagcgcca gagcgaagcc ggtggcgccc ggcaagcgag tgaccttcgc
     1681 cctcaagttc cccggcaagc tcaagcagaa gctggaggag ctggggccaa aacagaagct
     1741 gaccctgaag atcaccgcca gcgccaccaa cgtcgccggt cagctcagca ccgacaaact
     1801 caaggtgaag ttgaagggac agggctagct ctcgaccgtg ggtcgatggg tctatgcttg
     1861 cgccgcaatg gccaggcgtt ttgtatgcgg tttgctcgtc gcgctcttcg ggtgcgcggc
     1921 gtgtggcgcg gcaagcgccg gggccgcgac cgaaatcggc agcgggtgcg atccggactc
     1981 caccggcggc tcggcgaaaa tggtcctgca actgggtcgt gtaagcgctc ctgccgcggt
     2041 caaaaccacg ggtccgggag tcgtcacctc gtggcgcgtt cagtcggctc cgttcctgga
     2101 accagccacc atggctctca aggttctccg tgagaccagc ggggaatttg aggtcctcgc
     2161 cgagagcgcc cccggcttgg ttctcggtca acccgaaaac aagttcccga cgcggatccc
     2221 cgtcagcgcg ggagccctct tcggattgag ttcctcgaaa tcgatcccga cctgcttcgg
     2281 aatcggcgcg ccaggggaca cgcttgcctc cttcaacccg gacgtcccgg tcggtggcaa
     2341 agcaaaacct tttgaaagcc ccgacgggat cctgctctcg ctagccgtcg tgatcgagcc
     2401 cgacgcggac ggcgacggct atggcgacga gagccaggac gggtgccccc agagcgctgc
     2461 gacgcagggc acctgtccgg ctccgcctcc cgtcgtcgtt accaaactgg tggggaagcc
     2521 gaaactggaa ggcaacgtcg tcgccgtgaa gctgacgacg agcgctcccg cgaaggtgac
     2581 cgtgaccggc tcgatccgcg gcaagcgggc cgctggtccc gcttcgaaaa ccgtgactcc
     2641 cggagaaacc gggcggctct acctgcagct ctcgaaagct acgaaacagc ggctggcgaa
     2701 actgccgcgc aaacgcaagc tgaagatggt cgtcgaagcg aaggtggccg gagcgccgac
     2761 cgtcagctcc gaaatcagct tgccggggcg gaagaaacca cagccgcctc gcgccccgcg
     2821 ctgagcgcca gggctacagc cccagaggcc aacgcggcga tcatcgcgaa caggtagccg
     2881 tcgtcggagg cgctgagcgc cgcggagagc gactcggggt cgccgacgat cgcgaccaag
     2941 atcgccgtgc cgagcacggc gccgacctgg cggaaagcgg cgttgacggc gctggcggtg
     3001 gcgaagcggt cgaccgggac gtcgcggacg gcggcggcac cgagggtcgg gaaggcgagt
     3061 ccgatcccga tcccggtgag cagcgcgccc ggcagccaga ggccgagcca gtcgggctcg
     3121 gcgccggcgg tgccgagcac ccagaccccg atcgcgaaga ggatcgtgcc ggggacgatc
     3181 acggcgcggt ggccgaagcg gtcggcgagg cggccggccg ggccggcgac cacggtcgac
     3241 atcgccgggc ccgggagggt ggcggcgccg gcctggagca cggtgtagcc ccagatctgg
     3301 gtgaggaaga ggacgttgcc gaggatcagc gagaagaagg cggaggcgaa gaggatcgtg
     3361 ccgacgttgc cgacgacgaa ggagcggtgg gcgaacagct ccggttcgac gatcggccgc
     3421 ggatggctgc ggctgcgggc gacaaccgcg agcaggagca gggcggcgac ggcgaagcag
     3481 gccagggtcg cgggcgagcc ccagccccag tcgttgccct cgacgatcgc cagggtcagc
     3541 gccgcgaggc cgagcgccag cagcccggcg ccgggcaggt cgggcaggcg actgtcgagg
     3601 ctcttcggct cgggcagcca gcg
//
LOCUS       DASRIG010000152         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_332770, whole genome shotgun sequence.
ACCESSION   DASRIG010000152 DASRIG010000000
VERSION     DASRIG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332770"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFB52_04145"
     CDS             <1..1068
                     /locus_tag="VFB52_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZO05554.1"
                     /translation="SLLVVAATPFIAGVQFMVLFDRALHTNFFQAAFGGDVISYQHIF
                     WFYSHPAVYIMMLPGFGIISEVISTHARKPIFGYRLMALALIGIVVLSYSVWAHHMFV
                     AGMFSWLRVPMMITTLLIAVPTGIKIFSWLGTLYFGKIHTYSTAMLFALAFIVTFTIG
                     GISGVMLGMVPIDIHVSDTYFIVAHIHFVLFGGSVFTIFAGIYHWFPKMTGRMYDERL
                     GRIHFWGTVVGTWATFIPMHWVGMDGMPRRVADYATQFGEWNLLISISAFVLGAVQLV
                     FLYNMIVSWRFGPKASANPWRANSIEWQVSSPPPVFNFDEIPRVVGGPYEYGVPGAKH
                     AIMSSEGAEVPAGEPSVVGAK"
     gene            1065..1961
                     /locus_tag="VFB52_04150"
     CDS             1065..1961
                     /locus_tag="VFB52_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05555.1"
                     /translation="MSEGEKRPLLVFVNEVAGGRKLLQAVRERQDQVSGVVVAAPQNQ
                     PSVGQLIDAGELREAARARVEVTMAVLAEFGIDSVGEVMDPDSSLALDDAVRAHQPGE
                     VLLSCLYDSRFGLTRKDLVEWAREKVEPEAKLTHIPVRIEDDAIRWDLIHTLVVATQT
                     VSTPDLVNRLKERAATSPHRYTIVCPAAEDVSEPEIVRDLASTLAQLYKAEIDATGQP
                     MSPDPFQAVKNAIEHYRIDEVLISTFAGESSRWLEDDLIGRVREITEKSVEHVEVGRP
                     AAAVAAAVAESGAAADAEGGES"
     gene            1961..2590
                     /locus_tag="VFB52_04155"
     CDS             1961..2590
                     /locus_tag="VFB52_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZO05556.1"
                     /translation="MESATVPVGHGHAEDHHHGPPEANQSSRIDKQVLGILLFIVSEV
                     MVFGSFFAAYFFLRVVVDPPSWPPEPYELPVGVAGVNTAILVSSSFTIHYALESIRRG
                     NRTGLKVGLVLTWILGATFLFIQMNEYAHIGFSARDLAFGSIFYSLTGLHGAHVFVGL
                     LLLTFATIRAFRGHFGPKAEDHLGVEVPGIYWHFVDVMWIVVYVTVYIL"
     gene            2634..3446
                     /locus_tag="VFB52_04160"
     CDS             2634..3446
                     /locus_tag="VFB52_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05557.1"
                     /translation="MRGKGAKFVALGTLMVVLVACGAAGADDGPAPPWEQAKLDATVF
                     PKRLPSDGFSPVRLELSSWVRMKDERWPPPLRELSFHLDRDVRFDLAGIPTCGDGGVH
                     SEIATSYSSLERECADALVGRGEAVVASSLVGDTVVRERIPLIVVNAVVPGEVPGEGR
                     TRLYVVGGLSIPVPITFVAPVYRQKRRHGDLGTVLRVVFPKLVSGAGSLNWLRLRINR
                     RTVVDGGREHAVVAARCPDRAFQANLEATYVYPQSGGEYAHSEQRVRRCGRR"
     gene            3480..3668
                     /locus_tag="VFB52_04165"
     CDS             3480..3668
                     /locus_tag="VFB52_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05558.1"
                     /translation="MRLNEDAVFKWVVIIGAAAATVIALTLLTNQLVGALWGLVLVIV
                     GSVYLYRWLARKWPEWRR"
     gene            3721..3915
                     /locus_tag="VFB52_04170"
     CDS             3721..3915
                     /locus_tag="VFB52_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05559.1"
                     /translation="MAGVREKWTDERLDDLNRKVDDGFRRTDERFIHLERTMNQWFIT
                     LVGLMVTGFVGMAGLILTQG"
     gene            complement(3902..4675)
                     /locus_tag="VFB52_04175"
     CDS             complement(3902..4675)
                     /locus_tag="VFB52_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05560.1"
                     /translation="MLPFWLSIGALSLVEAALVALPKPAIAPLLERLQSKWWALALPL
                     PIAIVVGGIALEEASADFLTYLALFAVPPLAALALALIVRGARPPLALLVVPLFAVAW
                     AWKGDLGGHTAALVLSALACTSLGWLLVCVVPARWVKLGIYAMAAIDAYLVGSNLLQE
                     PNATLNAAAPAAGLPKLQLAHFGSALMGFGDLFIAATLGALLAYDRRLQLTATAIAAV
                     IGVSFDFLFFALDTLPATVPIALTLAILELSGRSRTNPG"
     gene            complement(4691..5608)
                     /locus_tag="VFB52_04180"
     CDS             complement(4691..5608)
                     /locus_tag="VFB52_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZO05561.1"
                     /translation="MTAVALVSLALIVLTGAAVRLTGSGLGCPDWPKCHGGTVPPADV
                     NAVIEYGNRLLTGFVGLAVIAACGLAFYRRPYRRHLAWIGGLLPLGVLGQAVLGALVV
                     KFHLAPGLVMCHFILSMMLLDAAFALAWCSRYEPGERRKSTDFWGVWAVRALIPLGQL
                     TILAGTIATASGPHPGDHDGELVQRFDFYGGETLEWVVQRHAVLATIYGLTAIGVWFF
                     LRRPGGDRRAVKPLTVVLLLLALQGVVGGVQWALELPGWLVWIHIALATGNWLAMLWT
                     VAAAGQLEPREKPAPVEPAQPAGATSGVS"
     gene            complement(5713..6405)
                     /locus_tag="VFB52_04185"
     CDS             complement(5713..6405)
                     /locus_tag="VFB52_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZO05562.1"
                     /translation="MNRRPKLINLLLALVALGAIVVVATGCGTTTADPERGRVLFTQK
                     CGTCHALAQAGTSATNGPNLDHAFASARETGMTDDTIEGVVKAQVELPRPNNGDPAVS
                     MPKNIVTGQDLEDVAAYLGEWAGVPGAAPPKVPGGPGAQVFASKGCGSCHILAAAESG
                     GTTGPNLDEVLVAGTTLEQIHEDIVDPDKDIAQGYSKGIMPSNFGESLTEKELEDLVQ
                     YLFENTPAGKGK"
     gene            6545..>6751
                     /locus_tag="VFB52_04190"
     CDS             6545..>6751
                     /locus_tag="VFB52_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZO05563.1"
                     /translation="MTAVSLTVGPSHTLREAARMMVEKRTGAALVSDGESPEPGIVTE
                     RDLLHSIGAGEDPDVELVGEHMSEG"
BASE COUNT         1057 a         2238 c         2324 g         1132 t
ORIGIN      
        1 tcgctgctgg tggtcgccgc caccccgttc atcgctggcg tccagttcat ggtcctcttc
       61 gatcgcgccc tgcacaccaa cttcttccag gcggcgttcg gcggggacgt gatcagctac
      121 cagcacatct tctggttcta ctcgcacccg gccgtctaca tcatgatgct gccgggcttc
      181 gggatcatca gcgaggtcat ctcgacccat gcccgcaagc cgatcttcgg ctaccgcctg
      241 atggccctgg cgctgatcgg catcgtcgtg ctcagttact cggtctgggc ccaccacatg
      301 ttcgtcgccg gcatgttctc gtggctgcgc gtgccgatga tgatcaccac gctgctgatc
      361 gccgtgccga ccgggatcaa gatcttctcc tggctgggga cgctctattt cggcaagatc
      421 cacacctatt cgacggcgat gctgtttgcg ctcgccttca tcgtcacctt cacgatcggc
      481 gggatctccg gcgtgatgct cggcatggtg ccgatcgaca tccacgtctc cgacacctac
      541 ttcatcgtcg cccacatcca cttcgtgctg ttcggcggct ccgtgttcac gatcttcgcc
      601 gggatctacc actggttccc gaagatgacc gggcggatgt acgacgagcg gctcggccgc
      661 atccacttct ggggcaccgt ggtcggcacc tgggccacct tcatcccgat gcactgggtc
      721 ggcatggacg ggatgccgcg acgcgtcgcc gactacgcga cccagttcgg cgagtggaac
      781 ctgctgatca gcatctccgc cttcgtgctc ggtgccgtcc agctcgtctt cctctacaac
      841 atgatcgtca gctggcgctt cggcccgaag gcgagcgcca acccgtggcg cgccaactcg
      901 atcgagtggc aggtctcctc gccgccgccg gtcttcaact tcgacgagat cccgcgcgtg
      961 gtcggcggcc cgtacgagta cggggtgccc ggcgccaagc acgcgatcat gtcgagcgag
     1021 ggcgctgagg ttcccgccgg cgagccgagc gtggtgggag ccaaatgagc gagggcgaga
     1081 agcgcccgct gctggtcttc gtcaacgagg tcgccggcgg tcgcaagctg ctgcaggcgg
     1141 tgcgcgagcg ccaggaccag gtgtccggcg tggtcgtcgc ggccccgcag aaccagcctt
     1201 ctgtcggcca gctgatcgat gccggtgagc tgcgcgaggc cgcgcgggcc cgggtcgagg
     1261 tgacgatggc ggtgctcgcc gagttcggca tcgactcggt cggcgaggtc atggaccccg
     1321 actcctcgct ggcgctcgac gacgcggttc gcgcccacca gcccggcgag gtgctgctct
     1381 cctgccttta cgactcgcgc ttcggcctta cccgcaagga cctggtcgag tgggcgcgcg
     1441 agaaggtcga accggaggcg aagctgaccc acatcccggt gcggatcgaa gacgacgcga
     1501 tcaggtggga cctgatccac acgctcgtcg tcgcgaccca gaccgtctcg acccctgacc
     1561 tggtcaaccg gctgaaggag cgcgccgcga ccagccctca ccggtacacg atcgtctgcc
     1621 cggccgccga ggacgtcagc gagccggaga tcgtccgcga cctcgcctcg accctggccc
     1681 agctctacaa ggccgagatc gacgcgaccg gccagccgat gagccccgac cccttccagg
     1741 cggtcaagaa cgcgatcgag cactaccgga tcgacgaggt gctgatctcg accttcgccg
     1801 gcgagagctc gcgctggctc gaggacgacc tgatcggccg cgtgcgcgag atcaccgaga
     1861 agtcggtcga gcacgtcgag gtcggccgcc cggccgccgc cgtagccgcc gccgtagctg
     1921 agagtggcgc cgccgccgac gccgagggag gggagtcctg atggagagcg cgacggtacc
     1981 ggtcggtcac gggcacgccg aggatcacca ccacggcccg cctgaggcga accagagctc
     2041 gcggatcgac aaacaggtcc tcgggatcct gctcttcatc gtctcggagg tcatggtctt
     2101 cgggtcgttc ttcgcggcct acttcttcct ccgcgtcgtc gtcgacccgc cgagctggcc
     2161 gccggagccc tacgaactcc cggtcggcgt agccggcgtc aacacggcga tcctggtctc
     2221 gtcctcgttc acgatccact acgcgctcga gtcgatccgc cgcggcaacc gcaccgggct
     2281 caaagtcggc ctcgtgctca cctggatcct cggcgcgacc ttcctcttca tccagatgaa
     2341 cgagtacgcg cacatcggct tcagcgcccg cgacctcgcc ttcggctcga tcttctacag
     2401 cctcaccggc ctgcacggcg cccacgtctt cgtcgggctc ttactgctca ccttcgcgac
     2461 gatccgagcc ttccgcggcc acttcggccc aaaggccgag gaccacttgg gcgtcgaggt
     2521 accaggcatc tactggcact tcgtcgacgt catgtggatc gtcgtttacg tgaccgtcta
     2581 catcctctag gcgtctttcg ccaagtccgg gtgtccgcta gcctccggcg cagatgcggg
     2641 ggaagggggc gaagttcgtt gcgcttggca cgctgatggt cgtgttggtc gcctgcgggg
     2701 cggcgggcgc tgacgacggt ccggccccac cttgggaaca agccaagctc gatgcgaccg
     2761 tattcccgaa gcgtctcccg tctgacgggt tttcgccggt tcggctcgaa ctgagttcct
     2821 gggtgcggat gaaagatgaa cggtggccgc cgccgttgcg ggagttgagc tttcacctcg
     2881 accgggatgt gcgcttcgat ctggcaggga ttcccacctg cggtgacggc ggtgtccatt
     2941 cggagatcgc tacgtcctac agctcgctgg aacgcgaatg cgcagatgcg ctcgttggca
     3001 ggggtgaggc cgtagttgcg tcctctctcg tcggcgatac cgtcgttcgt gagcggattc
     3061 ccctgatcgt cgtgaacgct gtggtcccag gcgaagtccc cggcgagggg aggaccaggc
     3121 tttacgtagt tggtggtctc tcgatcccgg ttccgatcac gttcgtcgcc cccgtgtaca
     3181 ggcagaagcg ccgacacggc gatcttggca cggttctgag ggttgtattc cctaagttgg
     3241 tcagcggagc cggctcgttg aattggctga ggcttcggat caaccgccgc accgtggtcg
     3301 atggggggcg cgaacacgcg gtggtggcag cgcgatgccc ggaccgggcc ttccaagcca
     3361 acctcgaagc gacgtacgtc tatccgcaat cgggcgggga atacgcgcac tcggaacagc
     3421 gggtgcgcag gtgcggacgg cgctgagccc cgcagccctt tctgcgtaag gatctacgca
     3481 tgaggctcaa cgaggacgcg gtgttcaaat gggtcgtgat catcggcgcc gctgcggcga
     3541 cggtgatcgc gctgaccctg ctcaccaacc agctcgtggg cgctctgtgg ggcctggtgc
     3601 tggtgatcgt cgggtccgtc tacctctacc gttggctcgc gcggaagtgg cccgagtggc
     3661 gacgctaaga attcactaag cgcagacaat acgtcacatc tctccggcta gcctgctttc
     3721 atggcgggcg tgagagagaa gtggaccgac gagcggctcg atgacctcaa caggaaggtc
     3781 gacgacgggt tcaggcgaac cgacgaacgc ttcatccacc tggagcggac gatgaaccag
     3841 tggttcatca ccctggtcgg gctgatggtc accggcttcg tcggcatggc cgggctgatc
     3901 ctcacccagg gttagttcgg ctccgcccgc tcagctccag gatcgccagc gtcagcgcga
     3961 tcgggacggt tgccggcaag gtgtcgaggg cgaagaagag gaagtcgaag ctgacgccga
     4021 tcacggcggc gatcgcggtg gcggtgagct gcaggcggcg gtcgtaggcg agcagggcgc
     4081 cgagggtggc ggcgatgaag aggtcgccga agcccatcag ggcggagccg aagtgggcga
     4141 gctgcagctt cggcaggccg gccgccgggg cggccgcgtt gagggttgcg ttcggctcct
     4201 ggaggaggtt ggagccgacc aggtaggcgt cgatcgcggc catcgcgtag atcccgagct
     4261 tgacccagcg cgccggcacc acgcagacca gcagccagcc gaggctggtg caggcgagcg
     4321 ccgagagcac cagcgccgcg gtgtggccgc cgaggtcgcc cttccaggcc caggcgaccg
     4381 cgaacagggg gacgacgagc aaagcgaggg gcgggcgggc accgcgcacg atcagggcga
     4441 gcgccagggc cgcgagcggc ggcaccgcga acagggccag gtaggtgagg aagtcggcgc
     4501 tggcttcctc gagcgcgatc cccccgacca cgatcgcgat cggcagcggc agggcgagcg
     4561 cccaccactt gctctgcagg cgctccagca ggggcgcgat cgccggtttc ggcagcgcca
     4621 ccagcgccgc ctcgaccagg ctcagcgccc cgatcgagag ccagaacggg agcatggtcc
     4681 cgcccgcctc ctagctgacg ccggaggtag cgccggcggg ttgcgccggc tcgaccggcg
     4741 ccggtttctc ccggggctcg agctggccgg cggcagccac ggtccagagc atcgccagcc
     4801 agttccccgt cgccagggcg atgtggatcc agaccagcca gccgggcagc tccagagccc
     4861 actggacgcc tcctacgacg ccctggaggg cgaggagcag caggaccacc gtgaggggct
     4921 tcacggccct cctgtcgcct cctgggcggc gcaggaagaa ccagacgccg atcgccgtca
     4981 ggccgtagat cgttgccagc acggcgtgac gctggaccac ccattcgagc gtctcgccgc
     5041 cgtagaagtc gaagcgttgc accagttcgc cgtcgtggtc gccggggtgg gggccggagg
     5101 cggtcgcgat cgtgccggcg aggatcgtca gctggcccag cgggatcagc gcccgcaccg
     5161 cccagacgcc ccagaagtcg gtcgacttgc gccgctcacc cggctcgtag cgcgagcacc
     5221 aggcgagcgc gaaggcggcg tcgagcagca tcatcgagag gatgaagtgg cacatcacca
     5281 ggccgggcgc gaggtggaac ttgaccacca gggcgccgag gaccgcctgg cccaggaccc
     5341 caagcggcag cagccccccg atccaggcga ggtggcggcg gtagggccgg cggtagaagg
     5401 cgagcccgca ggcggcgatc accgcgagcc cgacgaaccc ggtcagcagc cggttgccgt
     5461 actcgatcac cgcgttgaca tcggccggcg gcaccgtgcc gccgtggcat ttgggccagt
     5521 cggggcagcc gagcccggag ccggtcaggc gcacggcggc gccggtgagg acgatcaggg
     5581 cgagggagac gagcgcgacg gcggtcacct gcgcgtagcg ctcaggcgag atctcgagcc
     5641 gatttctcag gcgttctcgc aaccttcggt actccaggcg aagcgacccg gtggccgccc
     5701 gcgtcagacg ccctatttgc ctttgccggc gggcgtgttt tcgaacaggt actggacgag
     5761 atcttcgagt tctttttcgg tcagagattc gccgaagttc gacggcatga tgccttttga
     5821 gtaaccctgg gcgatgtctt tgtccgggtc gacgatgtcc tcgtggatct gctcgagcgt
     5881 ggtgccggcg accaggactt cgtccaggtt cggaccggtg gtgccgccgc tttcggcggc
     5941 ggcgaggatg tggcagctgc cgcagccttt gctggcaaag acctgggcgc cggggccacc
     6001 gggcaccttg ggcggcgcag cgccgggcac gccggcccat tcgccaaggt aggcggcgac
     6061 gtcctcgagg tcctggccgg tgacgatgtt cttcggcatc gagaccgccg ggtcaccatt
     6121 gttgggacgg ggcagctcga cctgcgcctt gaccacgcct tcgatcgtgt cgtcggtcat
     6181 cccggtttcg cgggccgagg cgaacgcgtg gtcgaggttc ggcccgttcg tcgccgaggt
     6241 gccggcctgc gcgagggcgt ggcaggtgcc gcatttctgc gtgaacagga cccgcccgcg
     6301 ctccggatcg gcggtggtcg tgccacagcc ggtcgcgacc acgacgatcg ctcccagggc
     6361 gaccagggcg agcagcaagt tgatgagttt ggggcggcgg ttcatacgtc tcgaagcgag
     6421 gcggagtcta tatgtcgagg ccccttgaaa gcgttcgtgt aggcggcacg gtttcgggta
     6481 ctctcgatcc gatccgcact aactctctgg gagcgaggag gactggatgc aggttcgaga
     6541 cgggatgacc gctgtatcgc tgacggtggg cccctcgcac acgttgcgcg aggctgcgcg
     6601 gatgatggtt gagaagcgca ccggggccgc tctcgtctcc gacggcgagt ctcccgagcc
     6661 cggaatcgtc accgagcgcg acctcctcca ctcgatcggc gccggcgagg atcccgacgt
     6721 cgagctggtc ggcgagcaca tgagcgaggg c
//
LOCUS       DASRIG010000153         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2944098, whole genome shotgun sequence.
ACCESSION   DASRIG010000153 DASRIG010000000
VERSION     DASRIG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944098"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFB52_04195"
     CDS             <1..201
                     /locus_tag="VFB52_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05564.1"
                     /translation="VGDEGAGVSSPSRELAGAAVALIPQVHEPGSLLALWEERDDVAT
                     ELFAWHCVARLRLAATGDLPIA"
     gene            310..1632
                     /locus_tag="VFB52_04200"
     CDS             310..1632
                     /locus_tag="VFB52_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05565.1"
                     /translation="MKRLTAALAAVAIVAAIAVPSAAASSIAYVASDGNVHLVAPDGS
                     RDLPLTTNATPDAKYRSPSQLDDGRVMAVGKSNGTSSFAFFFARDGQMLESRLLPSSG
                     SGGFAPYSEAQASPDGGVIAYDYRHFDCGVFPCQGNQRVGFVAGPGQTNPCLINCHVG
                     YVAPRWVPGTPYAAMVGQSFNAVYVQNGGASPVGWFTYEGEVRVFGLDLRANKIVTSV
                     GEGDTEYMVLEQMTGAPPAVPQSQCSVSMPDGARPRLSPDGSMVAWSAPEGVMVSPTP
                     AQSGGIGTLCQLTPKLIAAGGSEPEWGVADLPAAPGNGSDDKGKGKGGAEGAKLTNVV
                     TPKLAKALGNGFGVGFRCAGPCKVNATATVAKAVARRYGLGNAVTKVGAGKKTLKAAG
                     AASVKIAFTAKAKGKLAGAASVPLKVTLAITDPGGGKRKLSKTVTLKR"
     gene            1658..1975
                     /locus_tag="VFB52_04205"
     CDS             1658..1975
                     /locus_tag="VFB52_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05566.1"
                     /translation="MALAARFSPRLGLALTLVWVALLALTGAEHALLFLAPALLIAIP
                     LLGGHYVGEELIARLVRRRRRPRPARALVARRVAPAPAAPRGAALLALGLAVRPPPRF
                     GCC"
     gene            2006..2605
                     /locus_tag="VFB52_04210"
     CDS             2006..2605
                     /locus_tag="VFB52_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HZO05567.1"
                     /translation="MNRKLTLALVALLALVAVPAGASAHVTLQPEDGAPGEYAVFAVR
                     VPNESENATTTRVAVSFPPGFEWASYEPVAGWRAEVSTYGEEVDGIVWTATGPGVGPG
                     EFVDFPIEIQVPEKPGAALAFRTVQTYDDGEVARWAGGPEAEMPAPQLTVSVGEAAQP
                     DRPDDSDDSGDTLAIVALVVGALGLLAGAAALLRSRRSG"
     gene            2689..>3143
                     /locus_tag="VFB52_04215"
     CDS             2689..>3143
                     /locus_tag="VFB52_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZO05568.1"
                     /translation="MNFVKRWSRQSGRDLTYAFGMLGTSVIAFCVWVTALSVTVSLLV
                     FVVGVFVWWGSVYLFRWTAWLDRQLAGWIREEEIPAVYRRPREPGVLAALRCVTTDPQ
                     TWRDLGWLVLSSVVGFCLATVALTATTIVLAYLTLPIWFSFIPDPEQQYG"
BASE COUNT          430 a         1119 c         1120 g          474 t
ORIGIN      
        1 gtcggggacg agggggccgg tgtttcgtcc ccgtcccggg agctcgcggg cgccgcggtc
       61 gcgctgatcc cgcaggtcca cgagccgggg agcctgctgg cgctatggga agaacgcgac
      121 gacgtcgcga ccgagctttt cgcctggcat tgcgttgccc ggctgcgctt ggcggccacc
      181 ggcgacctgc cgatcgccta gccgggcccc tgctgatctt ggggtaaggg tttaggggtc
      241 accccattgt tggggcgctc ttgcggcgaa aagctgactg ggatgacgca accccagcaa
      301 ggagacccaa tgaaacgcct caccgcagcg ctcgccgcgg tggcgatcgt ggccgcgatc
      361 gcggtcccga gcgccgctgc gagcagcatc gcctacgtcg ccagcgacgg caacgtccac
      421 ctggtcgcgc cggacggctc gcgtgacctg ccgctgacca cgaacgcgac cccggacgcc
      481 aagtaccgct cgccctcgca gctcgacgac ggtcgggtga tggcggtcgg caagtcgaac
      541 gggacctcct cgttcgcctt cttcttcgcc cgtgacgggc agatgctgga aagccggctg
      601 ctgccgagct ccggctcggg cggcttcgct ccgtacagcg aggcccaggc ctcgccggac
      661 ggtggcgtga tcgcctacga ctaccgccac ttcgactgcg gggtctttcc ctgccagggc
      721 aaccagcggg tcgggttcgt cgccgggccc ggccagacca acccctgcct gatcaactgt
      781 cacgtcggct acgtcgcccc gcgctgggtg cccggcacgc cctacgcggc gatggtcggc
      841 cagagcttca acgcggtcta cgtgcagaac ggcggcgcct ccccggtcgg ctggttcacc
      901 tacgaaggcg aagtccgggt cttcgggctt gacctgcggg cgaacaagat cgtcaccagc
      961 gtcggcgaag gcgacaccga gtacatggtg ctcgagcaga tgaccggcgc gccgccggcg
     1021 gtgccgcagt cgcagtgcag cgtcagcatg cccgacggcg cccgcccgcg gctctcgccc
     1081 gacggctcga tggtcgcctg gagcgctccc gagggcgtga tggtctcgcc gaccccggcc
     1141 cagagcggcg gcatcggcac gctctgccag ctcaccccga agctgatcgc ggcgggcggc
     1201 agcgaaccgg agtggggcgt cgccgacctg cccgccgcgc ccggcaacgg ctccgatgac
     1261 aagggcaaag gcaaaggtgg cgccgagggc gcgaagctga ccaacgtggt cacgccgaag
     1321 ctggcgaagg cgctcggcaa cggcttcggc gtcggcttcc gctgcgccgg tccctgcaag
     1381 gtgaacgcga ccgcgaccgt cgccaaggcc gtggccaggc gctacgggct cggcaacgcc
     1441 gtgaccaagg tcggcgccgg caagaagacg ctgaaggcgg ccggtgcggc gagcgtgaag
     1501 atcgccttca ccgcgaaggc gaagggcaag ctggccggcg ccgccagcgt cccgctcaag
     1561 gtgaccctgg cgatcaccga ccccggcggc ggcaagcgca agctgagcaa gacggtgacc
     1621 ctgaagcgct gattccgtcc tcgatagcct gggcgagatg gcgctcgcgg cccgtttctc
     1681 gcccaggctt ggcttggcac tgacgctcgt ctgggtcgcg ttgctggcgc tcaccggggc
     1741 cgagcacgcg ctgctcttcc tcgccccggc gctgctgatc gcgatcccgc tgctcggcgg
     1801 ccactacgtc ggcgaggagc tgatcgcgcg gttggtgcgg cgtcgccgcc gcccgcgccc
     1861 ggcccgcgcc ctggtcgccc gccgggtcgc tccggcgccg gcggcaccgc gtggcgcagc
     1921 gctgctcgcg ctcggcctgg cggtgcgccc gccaccccgt ttcggctgct gctgaaccag
     1981 agctgaaacg aacgaaagga acgcgatgaa tcgcaagctc acccttgcgc tggttgcgct
     2041 actcgccctc gtggcggtgc cggccggcgc cagtgcccat gtcaccctgc agcccgaaga
     2101 cggagcgccc ggcgaatacg ccgtgttcgc cgtgcgggtg ccgaacgaga gcgagaacgc
     2161 gaccacgacc agggtcgcgg tcagcttccc gcccgggttc gagtgggcct cctacgaacc
     2221 ggttgccggc tggcgggcgg aggtctcgac ctacggcgag gaagtcgacg ggatcgtctg
     2281 gacggcgacc ggcccgggcg tcggcccggg cgagttcgtt gacttcccga tcgagatcca
     2341 ggttccggaa aagccggggg ccgcgctcgc cttcaggacg gtgcagacct atgacgatgg
     2401 cgaggtcgcg cgctgggccg gtggcccgga agcggagatg ccggcgccgc agctgaccgt
     2461 gagcgtgggc gaagccgcgc agcccgatcg cccggacgac tccgacgact ccggtgacac
     2521 gctcgcgatc gtggccctgg tcgtcggtgc cctcggcctg ctggccggcg ccgccgccct
     2581 gctgcgctcg cgccgctccg gctgatccgg agggggtggg gccagccgta ccttgagacg
     2641 ctggctggcc ccattccatc gcgcggcgcg ggaggcgatg atctgcgcat gaacttcgtg
     2701 aagagatgga gcaggcagtc cgggcgtgac ctcacctacg ccttcgggat gctcggcacc
     2761 agcgtgatcg ccttctgcgt ctgggtgacc gcgctcagcg tcaccgtctc gctgctggtc
     2821 ttcgtggtcg gcgtcttcgt ctggtggggc agtgtctatc tgttccgctg gaccgcctgg
     2881 ctcgaccgtc agctggccgg ctggatccgg gaggaggaga tcccggcggt ctaccggcgc
     2941 ccgcgcgagc ccggtgtgct ggcggcgctg cgctgcgtca ccaccgaccc gcagacctgg
     3001 cgcgacctcg gctggctggt gctcagctcc gtggtcggct tctgcctcgc gaccgttgcc
     3061 ctgaccgcga ccacgatcgt gctcgcctac ctgaccctgc cgatctggtt cagcttcatc
     3121 cccgatcccg agcagcagta cgg
//
LOCUS       DASRIG010000154         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2944389, whole genome shotgun sequence.
ACCESSION   DASRIG010000154 DASRIG010000000
VERSION     DASRIG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944389"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFB52_04220"
     CDS             complement(<1..490)
                     /locus_tag="VFB52_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05569.1"
                     /translation="MGRPVVVSAKEEEMIRMHVRMTGAIALCLALGVSALALADGAGD
                     NEAFVDGSVKPRKLDKKKHKPIALFTEVRTEGPVTGNQENPEKELIEFDRDGAWKSNA
                     APLCAANIETPGLTVEQARDLCPAGSYIGSGEAEVNLGPARISNITVSVFNGPAKNQI
                     RLH"
     gene            complement(559..1287)
                     /locus_tag="VFB52_04225"
     CDS             complement(559..1287)
                     /locus_tag="VFB52_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05570.1"
                     /translation="MIRKSRMAVALGLVLALGVATVAWADGATDNTPAVDGSVNPSKL
                     DKKKYKPVNLLVGVRTEGPVTGTQENPEAEYISFGKNIKWQSTAAPFCTAAIETPGLT
                     ADQARAMCPGKSLIGTGEAEVNLGASRVSNITVQVFNGPGKNQVRLHTSSPTLGAAAP
                     TVAGSIVKSNAGSKYGNALVVADAPDAGGDAFLITKFNATIKKGSKVATARCKAKKFL
                     WQRVVTYDDGTKETATLQQPCKRK"
     gene            complement(1420..>2240)
                     /locus_tag="VFB52_04230"
     CDS             complement(1420..>2240)
                     /locus_tag="VFB52_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05571.1"
                     /translation="RNEDLATAFEIFPTFLRESRATLTRLETFAADSDPVVQALQPTA
                     RELAPTVTSLARLSDQLDPFFVGLRGTIDAAPKGSAALRRLLDADLPPLLDRFDPFLA
                     ELNPILEVVRIYRHEVTALLANVSASTNGLLASSAGVENYLRTLVMQTPEAVTTYPNR
                     LQINRTNPYLKPKAALDVRTGLASFETRQCAAGTVATLPERAATIADPAFNDRTDGDL
                     DDAGDFYNRIQLYAFNDQPSSATISPPPCNQQSPFDSIGAPSENSNYLHVYPFP"
BASE COUNT          322 a          721 c          780 g          417 t
ORIGIN      
        1 tatgcaggcg gatctggttc ttcgccgggc cgttgaacac cgagacggtg atgttgctga
       61 tgcgcgccgg accgaggttg acctcggcct cgccgctgcc gatgtacgac ccggcggggc
      121 acaggtccct ggcctgctcg acggtcaggc cgggggtctc gatgttcgcg gcgcaaagcg
      181 gggcggcgtt cgacttccac gcgccgtcgc ggtcgaactc gatcagctcc ttctcggggt
      241 tctcttggtt tcccgtcacc ggcccctcgg tgcgaacttc cgtgaacagc gcgatcggct
      301 tgtgcttctt cttgtcgagc ttcctcggct tgaccgagcc gtcgacgaag gcctcgttgt
      361 cgccggctcc gtcggcaagg gcgagtgctg agaccccgag cgccaggcat agcgcgatcg
      421 cgccggtcat ccgaacgtgc atgcgaatca tctcctcctc cttggccgaa acgacgacgg
      481 gccggcccac tgggccggcc cgtctcggac gttcagtcga tacgcaagtg agcgcgggct
      541 agttgccgcc ctttaccgct acttgcgctt gcacggctgt tggagcgtgg cggtctcctt
      601 ggtgccgtcg tcgtaggtga ccacgcgctg ccacaggaac ttcttcgcct tgcagcgagc
      661 tgtggcgacc ttcgagccct tcttgatcgt cgcgttgaac ttcgtgatca agaacgcgtc
      721 accgccggcg tccggggcgt cggcgacgac cagcgcgttg ccgtacttgc tgccggcgtt
      781 ggacttgacg atcgaccccg cgaccgtggg cgcggcggcg ccgagcgtcg ggctcgaggt
      841 gtgcagccgg acctggttct tccccgggcc gttgaagacc tggaccgtga tgttgctgac
      901 ccgcgaggcg ccgaggttga cctcggcctc gcccgtcccg atcagggact tgccggggca
      961 catcgcgcgc gcctgatcgg cggtgagacc cggcgtctcg atcgcggcgg tgcagaacgg
     1021 cgccgcggtg ctctgccact tgatgttctt gccgaacgag atgtactccg cctcggggtt
     1081 ctcctgggtt ccggttaccg gaccctcggt gcgaacgccg acgagcaggt tgaccggctt
     1141 gtacttcttc ttgtcgagct tgcttgggtt gacggagccg tcgaccgccg gcgtgttgtc
     1201 cgtcgctccg tccgcccagg caaccgtcgc aacgccgagc gcgagcacga ggccgagcgc
     1261 gaccgccatg cgtgacttac gaatcactac ttcctcctat cgaaacgcgc ggccgcacgg
     1321 cacgcgcaaa ttacctctct ccagagtcct ccgaggccac ctgaggcggc cagcggcacc
     1381 accttacaac ccctgcgggg gcggattgtt tcgcttcgat cacgggaatg ggtacacgtg
     1441 caggtagttg ctgttctcgc tcggcgcccc gatcgagtcg aacgggctct gctggttgca
     1501 gggcggcggc gagatcgtgg ccgatgacgg ctggtcgttg aacgcataaa gctgaattcg
     1561 gttgtagaag tcgccggcgt cgtcgaggtc gccgtcggtc cggtcgttga acgccgggtc
     1621 ggcgatcgtc gccgcccgct cggggagcgt cgcgaccgtc ccggccgcgc actggcgggt
     1681 ctcgaacgag gcaagccccg tgcgcacgtc aagcgccgcc ttcggtttca aatacgggtt
     1741 cgtgcggttg atctgcagcc ggttggggta cgtcgtcacc gcctcgggcg tctgcatgac
     1801 gagcgttcgc aggtagttct cgaccccggc gctgctggca agcagcccgt ttgtcgaagc
     1861 ggagacgttc gcgagcaggg cagtcacctc atgccggtag atgcgaacca cctcgaggat
     1921 cgggttgagc tcggcgagga acgggtcgaa gcgatcgagc aggggcggaa gatcggcatc
     1981 gagtaggcgc ctgagcgccg ccgagccctt cggggcggca tcgatggtcc cgcgcagacc
     2041 cacgaagaag gggtcgagct ggtcggaaag gcgggcgagc gaggtgacgg tcggggcgag
     2101 ctcgcgcgcg gtcggctgca aggcctggac caccgggtcc gagtcggcgg caaaggtctc
     2161 gagccgggtc agggtcgcgc gcgattccct caggaaggtg gggaagatct cgaatgccgt
     2221 cgccaggtcc tcgttgcgcc
//
LOCUS       DASRIG010000155         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3193267, whole genome shotgun sequence.
ACCESSION   DASRIG010000155 DASRIG010000000
VERSION     DASRIG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193267"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFB52_04235"
     CDS             <1..182
                     /locus_tag="VFB52_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05572.1"
                     /translation="EPTGGDAERHLAALEKAGLGGDANAAQLLAGFQRGSQRAAQLVA
                     ELTDAPILVPAPGSG"
     gene            234..614
                     /locus_tag="VFB52_04240"
     CDS             234..614
                     /locus_tag="VFB52_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05573.1"
                     /translation="MSVSVSGVDFLTVLVKDYPAAQKFYTEVLGLEHSVDYEKIPAGE
                     YETGNLTLQVMDAASIGREFEPRTHPLALHVDDVEAARADLEAKGVNFVAETIDSGVC
                     HMAIFEDPDGNVLMFHNRYAPKAG"
     gene            complement(611..1222)
                     /locus_tag="VFB52_04245"
     CDS             complement(611..1222)
                     /locus_tag="VFB52_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO05574.1"
                     /translation="MRARLLLGGVGAAVLGAALWWRKNPSACPYGQRFWVEAPHPIIT
                     RERLRAVLRPEPGERILEIGVGTGYYSHDIAGWIEPEGTLELFDLQQEFLDHVMRGAA
                     ERDLTNLVPTQGDATALPFPDASVDAVVLTAVLGEIPDSDAALREIRRVLKPDGRLVV
                     GELFGDPHFTTQAALERQGAAAGLRLDERSGNWFGYFGRLAPV"
     gene            complement(1222..1443)
                     /locus_tag="VFB52_04250"
     CDS             complement(1222..1443)
                     /locus_tag="VFB52_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05575.1"
                     /translation="MAEAQRVEIGFEGGQVVSTRLTDDDLKDLRKQLEKGGWHDVHTE
                     DGVLAVYLPKVAFLRIASGSSRVGFGSVE"
     gene            complement(1443..1853)
                     /gene="mce"
                     /locus_tag="VFB52_04255"
     CDS             complement(1443..1853)
                     /gene="mce"
                     /locus_tag="VFB52_04255"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZO05576.1"
                     /translation="MFGRIDHVGVAVEDLDAALELYEAGFQMPLVHRETVTEQGVEAV
                     LLDVGDGHVELLAPLGPDTPVGKFMAKNGAGLHHVAYAVDDIDATLERLAETGIKLID
                     TKPRIGIRESRVAFLHPRSTGGVLTEIVEPAGGH"
     gene            complement(1864..>2146)
                     /locus_tag="VFB52_04260"
     CDS             complement(1864..>2146)
                     /locus_tag="VFB52_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZO05577.1"
                     /translation="VMLERLVDEPGPAVRAGVGLAGASLAVEMFAWSDRHHGDPLAEA
                     FHTPGREIQRHWATKEPTPEQLEVGLAALAEILRVEEPPGEQTEPPPAA"
BASE COUNT          325 a          749 c          773 g          299 t
ORIGIN      
        1 aggagccgac cgggggtgac gccgagcgcc acctggcggc ccttgaaaag gctggcctgg
       61 gtggcgacgc aaacgccgcg cagctcctcg ccgggttcca gcgagggagc cagcgcgccg
      121 cgcagcttgt cgccgagctc accgatgcgc cgatcctagt cccggccccg ggcagcggct
      181 agggtcgcgt caccgacccc caccatcacc gaccaccaat cagggagaga gcaatgagcg
      241 tttccgtcag cggcgtcgac ttcctcaccg tcctcgtcaa ggactatccc gcggcgcaga
      301 agttctacac cgaggtgctc ggcctcgagc actcggtcga ctacgaaaaa atccccgccg
      361 gcgagtacga gaccggcaac ctcaccctgc aggtgatgga cgcggcctcg atcggccgcg
      421 agttcgagcc gcgcacgcac ccgctggcgc tgcacgtcga cgacgtcgaa gccgcccgcg
      481 ccgacctcga ggcgaagggc gtcaacttcg tcgccgagac gatcgacagc ggcgtctgcc
      541 acatggcgat cttcgaagac cccgacggca acgtgctgat gttccacaac cgctacgcgc
      601 cgaaggcggg ctagaccggc gcgaggcggc cgaagtagcc aaaccagttg ccggagcgct
      661 cgtcgaggcg caggccggcg gcggcgccct ggcgctcgag tgcggcctgg gtcgtgaagt
      721 gggggtcccc gaagagctcg ccgacgacaa ggcgcccgtc cggcttcagc acccggcgga
      781 tctcgcgcag ggcggcgtcg gagtcgggga tctcgccgag caccgcggtc agcacgaccg
      841 cgtcgaccga ggcgtcgggg aagggcagcg cggtggcgtc gccctgggtc gggaccaggt
      901 tggtcaggtc gcgctcggcg gcgccgcgca tcacgtggtc gaggaactcc tgctggaggt
      961 cgaacagctc cagcgtcccc tccggctcga tccaacccgc gatgtcgtgg ctgtagtagc
     1021 cggtgccgac gccgatctcg aggatccgtt cgccgggctc ggggcgcagg accgcgcgca
     1081 ggcgctcacg ggtgatgatc ggatgcggcg cttcgaccca gaaacgctgc ccgtaggggc
     1141 aggcggaggg gttcttgcgc caccagagcg ccgcgcccag gaccgccgct ccgacaccgc
     1201 cgagcagcag tcgggcgcgc atcactcgac gctgccgaag ccgacccggc tggagccgga
     1261 ggcgatgcgc aggaaggcga ccttggggag gtagacggcg agcaccccgt cctcggtgtg
     1321 gacgtcgtgc cagccgccct tctcgagctg cttgcgaagg tccttgagat cgtcgtcggt
     1381 gagccgggtg gagaccacct ggccgccctc gaagccgatc tcgacgcgct gtgcctctgc
     1441 catcagtgcc ctcctgcggg ttcgacgatc tcggtcagaa ccccgccggt cgagcggggg
     1501 tgaagaaagg cgacgcggct ttcgcggatc ccgatccgcg gcttcgtgtc gatcagcttg
     1561 atcccggtct cggcgagccg ctccagggtg gcgtcgatgt cgtcgaccgc gtaggcgacg
     1621 tggtgcagac cggcaccgtt cttggccatg aatttgccga ccggcgtgtc ggggccgagc
     1681 ggggccagca gctcgacgtg gccgtcgccg acgtcgagca ggaccgcctc gacgccctgc
     1741 tcggtgaccg tctcgcggtg caccagcggc atctggaagc cggcctcgta gagctcgagc
     1801 gcggcgtcga ggtcctcgac ggcgacgccg acgtgatcaa tccttccgaa cacgaggggc
     1861 agtctatgca gccgggggcg gctcggtctg ctcgccgggc ggctcctcga cccggaggat
     1921 ctccgccagc gccgcgagcc cgacctcgag ctgctcgggg gtcggctcct tggtcgccca
     1981 gtggcgctgg atctcgcggc cgggggtgtg aaaggcctcg gccagcgggt cgccgtggtg
     2041 gcggtcgctc caggcgaaca tctcgaccgc gagcgaggcg ccggcgaggc cgacgccggc
     2101 gcgcaccgcg gggcccggtt cgtcgaccag ccgttcgagc atcacc
//
LOCUS       DASRIG010000156         7900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3194543, whole genome shotgun sequence.
ACCESSION   DASRIG010000156 DASRIG010000000
VERSION     DASRIG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194543"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFB52_04265"
     CDS             <1..673
                     /locus_tag="VFB52_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HZO05578.1"
                     /translation="YPEESGPFRGDYRDCPKGTPRCKPWLPKTKARAAEYALEWTDED
                     GEANTEDYWYFGNNNCTNFVSQAWFAAGQNAMYENLPRKNWPLNWWAERDRETPYEGA
                     NNNYNWTNVQGFRDQQVKSRRAIVLTEAKTRGKNLKVGDVVVIDFGDDEVWNHSYIVT
                     QSNEDGRFVSSQTTTRHNFPWEELATEILPRSAQFGSETGEWEKNAWDWQIVRPLYKG
                     SNVRR"
     gene            660..1184
                     /locus_tag="VFB52_04270"
     CDS             660..1184
                     /locus_tag="VFB52_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05579.1"
                     /translation="MCDAERRGGAVRLWLAIVAVGIGLLVPVVGCGEDGGAEVRPAAD
                     TVTTDAVVAALEGTGYQFRYREVPHVEGYEMVAGEARQGETRMQFAVEVRLAGPYSEA
                     GEERGNPQPQILRYGFGQHATVTGNIRYLTVSQAPRAARGGFELTSSKREQRMEVDVL
                     VALAKLFPPRLIGV"
     gene            1181..1735
                     /locus_tag="VFB52_04275"
     CDS             1181..1735
                     /locus_tag="VFB52_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05580.1"
                     /translation="MSVVRRKPLACLAAVVACLFVVGSVAAAEKVAIEGDPHVVSEQS
                     LKDELMKTGVAIQFHTVESGEVPSVVGVARDGGAVVGFEFQLYPSSDEATAWDLGELP
                     AAAFGWPPKYKDFLLKVEVRGVLGNVAFAEYERGVLESQSTVKEVNERQRSKQRVARA
                     LDDALFGLFPSSDPYVHALATVPG"
     gene            1797..2756
                     /locus_tag="VFB52_04280"
     CDS             1797..2756
                     /locus_tag="VFB52_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05581.1"
                     /translation="MATLLVLVAVAAWLAGPGAGRAAAKPADGTATVADVLTALEGTG
                     YQLRYRQVPQVAGYDLVVGEATHRGTALQFSFEIRHAGPFGTAVEEPVNPQPPIVRYD
                     LGSRGTVVGNVYIRTELQAPKRARGGFEIGHTKAESRIELDFVIAVERLFPAEWQQVP
                     RVAGDAGVTGGDPYLVSAERLRSELAGSGAKIRFAASRGGAIPSVVGVARDGGAVIGF
                     EFQLYPSSDVATVAAAGELRAARFGWPRRYRGFLLKVEVRGVLGNVVYTEYERGAVES
                     HSTVREVNRRQRARQRIMRALDDALFASYPEDDPFAVALLTEG"
     gene            complement(2708..4933)
                     /locus_tag="VFB52_04285"
     CDS             complement(2708..4933)
                     /locus_tag="VFB52_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05582.1"
                     /translation="MSQPRLSAPSGARTGRGLETVTRWVLAHKGLVAIFWIVLTVAGF
                     YGSSKVTDRLDEQFSMPSSPAYAVNQEIAERFGGGGEATPLVAVTTLPAGENVRDPGV
                     RSDLRQAERKLTAALPGARVASYGSTGEAAYVSDDGRTTFVLAYPPIEQGEGPGAEIS
                     PQTVEAARGALAKSEVGGSDVLLTGRAALEGESPEEEGGDSSFLNETLIAGVAALVVL
                     VFVFASALALVPLLMAVIAIPVTLLAVLGLTAVTDVSFIVLFLCSLIGLGLAIDYALI
                     VVMRWREERDRGLGNEEAVLAASRTAGHAVLFSGTTVAIGLLAAVVLPIPFLRSMAYG
                     GLLIPLVSVAVALTLLPVVLAKFGPRADRRRLRRTDRAERHWASWARLVVRHRVAAIV
                     FSLAVLLGLVGVASGMLLGDPEAKSMGGSGEAQAGLVALERSGIGASPLTPIEAVAPS
                     DEAAATARDLARVDGVRAATAPSTPGWSEDGRAVIDVFTSADTASSSGRDTVGAVRDA
                     AAELPDVGVGGSTAMIEDWVEEVYGSFPLMLAFIALVTFVLLVRAFRSVILPLKAVVM
                     NLLSVFATWGVMTLVWQHGFGTELLFGSEPTGSIAFWAPMIVFAFIYGLSMDYEVFIL
                     SRVREEYDRTGSTDEAIVRGIASTGRLVTSAALIVFLAFASMATGEEIDIKVLATGLA
                     AGVLLDALLVRSLLVPATVSLLGRWNWWMPDPVRRFLRVPETIDQPSVKSATANGSSS
                     G"
     gene            5068..6291
                     /locus_tag="VFB52_04290"
     CDS             5068..6291
                     /locus_tag="VFB52_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZO05583.1"
                     /translation="MLGLDRSSQRLRALTEHGPNAVGSSPAPGSSRYRLLDAGVVLAV
                     LAVSLVQLASHVFVDDDVAGELDDPDVLGVVLVLLTSLPLFWRRRHPWAVMAIAGGGG
                     IALVACGYSVNVPAALFVALYTFAASGRGATWPPIAFAGLLFVAQVVVEIAVLSLSIE
                     DYLIPALLLAGAWVFGERRHTAALREAEEHERREREERLSIAEERTRIARELHDSAGH
                     AINTILVQAGAARVLRERDPERSQEAIEAIERLALETIEDIDRIVGSLRAEGPAERAP
                     LPGIERIPDLVAEQRRAGLEVELRELADDWRKPPPAVDRAAYRIAQEALTNAARHGSG
                     RVELTLHQHVDRLELIVVNPIAAAPAPARPGGGGLGLIGMRERAQLLGGFLEAGPVDG
                     RFRVRAVFPYSWIRE"
     gene            6288..6974
                     /locus_tag="VFB52_04295"
     CDS             6288..6974
                     /locus_tag="VFB52_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05584.1"
                     /translation="MRPNPDSGRERGPIRVMIVDDDELMRTGLRAVLSTDDRIEVVEE
                     AGDGEQAVRRAGRTQPEVVLMDVRMPGQDGIAATADLLAVAPEAKVVILTTFEEDEYI
                     HGALRAGASGFLLKRTSPEDLTAAVHRVSAGEALLSPSVTRRVIDQMVARPLPRAGDD
                     PRLAELTAREREVLELIARGLSNREIAAQLVVEETTVKTHVKRVLAKLGVRDRVHAVI
                     VAYETGVVAP"
     gene            7026..>7900
                     /locus_tag="VFB52_04300"
     CDS             7026..>7900
                     /locus_tag="VFB52_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05585.1"
                     /translation="MRAFLLVPVAVLGLMAASPVVSAAETPGFELAKKGKACKGTKVA
                     VKQSGKTICKAFGQVFPKPRANDLRLAYLQQTLRLDPAELLKGKKAKRARTLQSGFGA
                     AGKRAQKKLLGLLPKALAFFDRKRAGARSSTLGAGATASANCGVGAAGDRGSLGGSST
                     VGMLGGNGLFIETNAGGGLRIRVTFFSCGGVDSFRIPECPTGEGNVVAQTATGDFSAT
                     TEVFRGGEFESRNTSRFEDTSKAKGKVGKDAKLKYIEVEHEQEVLIVASGGFVARGGM
                     KREVKIDMPAGKYDPA"
BASE COUNT         1298 a         2654 c         2859 g         1089 t
ORIGIN      
        1 gtacccggaa gagtcagggc ccttccgcgg cgactaccga gactgcccca aggggacgcc
       61 gcgctgcaaa ccctggctcc caaagacgaa agcaagggct gccgagtacg cgttggaatg
      121 gactgacgag gatggcgagg cgaacacgga ggactactgg tacttcggaa acaacaactg
      181 cacgaacttc gtcagccagg cttggtttgc cgccggtcaa aacgcgatgt acgagaacct
      241 cccgcgcaaa aactggcctc tcaactggtg ggccgagagg gacagggaaa ccccgtacga
      301 aggagccaac aacaactaca actggaccaa cgttcagggc ttcagggatc agcaggtgaa
      361 atcgcgccgc gccatcgtgc tgaccgaggc aaagaccagg ggcaagaatc tcaaggttgg
      421 cgacgtggtc gtgatcgatt tcggcgacga cgaagtgtgg aaccactctt acatcgtcac
      481 ccagtccaat gaggacggtc gcttcgtttc cagtcagacc acgaccaggc ataacttccc
      541 ctgggaagag ctcgctaccg agatactccc gcgttctgcc caattcggct ccgaaaccgg
      601 cgaatgggag aaaaatgcct gggactggca aatcgtgagg ccgctttaca agggttcgaa
      661 tgtgcgacgc tgagcgacgg ggcggcgccg ttcggctgtg gcttgcgatc gtcgccgtcg
      721 ggatcggcct gctcgtgccc gtcgtcggct gtggcgagga cggcggagcg gaggtgcgcc
      781 cggctgccga cacggtgacg acggacgcgg tggtcgccgc tctggagggg accggctacc
      841 agttccgcta ccgcgaggtg ccgcacgttg agggctacga gatggtcgcc ggcgaagcgc
      901 ggcagggcga gaccaggatg cagttcgccg tggaagttcg ccttgccggc ccctacagcg
      961 aggcgggtga ggagcgggga aatccgcagc cccagatcct ccgctacggg ttcggccagc
     1021 acgccacggt gaccggaaac attcggtatc tgaccgtctc gcaagcgccc agagccgccc
     1081 gtggtggatt cgaactgacg agctcgaagc gggagcagag gatggaggta gacgttctcg
     1141 tcgccttggc gaagctcttc cccccgaggc tgatcggcgt atgagcgtgg tccggcgcaa
     1201 accgctcgcc tgcctcgccg cggtggtggc ctgcctgttc gtcgtcggct cggtcgcggc
     1261 cgccgagaag gtcgccatcg aaggcgatcc acacgtggtc agcgaacaga gcctgaaaga
     1321 cgagctgatg aaaacggggg tcgcgattca gttccacacc gtggaatccg gggaggttcc
     1381 gagcgtcgtc ggggtcgcgc gcgacggcgg agcggtggtc ggcttcgagt tccagctcta
     1441 cccgagcagc gacgaagcga cggcctggga cctcggcgag ttgcccgcgg cggccttcgg
     1501 ctggccgccc aaatacaagg acttcctgct caaagtcgag gttcgcggcg tgctcggcaa
     1561 cgtcgccttc gccgagtatg agcgcggcgt gctcgaaagc cagagcaccg tcaaggaggt
     1621 caacgaacgg cagcggtcca agcagcgcgt cgcgcgagca ctcgacgacg ctctcttcgg
     1681 gttgttcccg tccagcgatc catacgtcca cgccctcgcg accgtccccg gctgacctcc
     1741 atactcgagc gaggatgttt ttaacccggg acggatctgc ctggcgccgc gcgaccgtgg
     1801 ccaccctgct ggtcctggtc gccgtcgctg cctggctcgc cgggccgggg gccggccggg
     1861 ccgcggcgaa gcccgctgac ggtaccgcca ccgtggcgga cgtcttgacc gccctggagg
     1921 ggaccggcta tcagctgcgg taccgccagg tgccccaggt tgccggctat gacctggtcg
     1981 tcggggaagc cacgcaccgg ggaaccgcgc tgcagttctc gtttgaaatc cggcacgccg
     2041 ggccgttcgg aacggcggtg gaggagccgg tcaatccgca gcccccgatc gttcggtacg
     2101 acctcgggag ccgtggcacg gtggtcggca acgtctacat ccgaaccgag ctgcaggcgc
     2161 cgaagcgggc tcgcgggggg ttcgaaatcg ggcacaccaa ggcggaatcc cggatcgagc
     2221 tggacttcgt gatcgccgtg gagaggctct ttcccgccga gtggcagcaa gtcccgcggg
     2281 tagccgggga cgctggggtc acgggtggag acccgtacct ggtctcggcg gagcgtctgc
     2341 gcagcgagct ggccgggtcc ggcgcgaaga tccgcttcgc ggcttcccgg ggcggggcga
     2401 tccccagcgt cgtcggcgtc gcgcgcgacg gcggggcggt gatcggcttc gagttccagc
     2461 tctaccccag cagcgacgtc gccaccgttg ccgccgccgg ggagttgcgc gccgcccgct
     2521 tcggctggcc gcgccggtac cgggggttcc tcttgaaagt cgaggtccgc ggcgtgctcg
     2581 gcaacgtcgt ctacaccgag tacgaacgcg gcgcggtcga aagccacagc accgtccgcg
     2641 aggtgaaccg gcgccagcgc gcccgccagc ggatcatgcg cgcgctcgac gatgcgctct
     2701 tcgcgtccta ccccgaggac gacccgttcg ccgtcgcgct cttgaccgaa ggttgatcga
     2761 tggtctccgg cacccgcagg aagcggcgca cggggtcggg catccaccag ttccagcggc
     2821 cgagcaggga gacggtcgcc ggcaccagca gcgagcggac caggagggcg tcgaggagga
     2881 cgccggcggc gaggccggtg gccagcacct tgatgtcgat ctcttccccg gtcgccatcg
     2941 aggcgaaggc gaggaagacg atcagggcgg cgctggtgac caggcggccg gtgctggcga
     3001 tgccacggac gattgcctcg tcggtcgagc ccgtgcggtc gtactcctcg cgcacccggg
     3061 agaggatgaa cacctcgtag tccatcgaga gcccgtagat gaaggcgaag acgatcatcg
     3121 gcgcccagaa ggcgatcgag ccggtcggtt cgctgccgaa gagcagctcg gtgccgaagc
     3181 cgtgctgcca gaccagagtc atcacacccc aggtggcgaa gacggagagc aggttcatca
     3241 ccaccgcctt caacggcagg atcacggagc ggaaggcgcg gaccaggagg acgaaggtga
     3301 cgagggcgat gaaggccagc atcagcggaa acgagccata gacttcttcg acccaatcct
     3361 cgatcatcgc ggtcgagccg ccgaccccga cgtcgggcag ctcggcggcc gcgtcgcgaa
     3421 ccgcgccgac ggtgtcgcgg ccgctgctgg aggccgtgtc ggcgctggtg aagacatcga
     3481 tcaccgcgcg gccatcctcg ctccagcccg gagttgacgg ggcggtggcg gcgcggaccc
     3541 cgtcgacccg ggcgaggtca cgggcggtcg ccgccgcctc gtcgctcggg gcgacggcct
     3601 cgatcggggt cagcggcgag gcgccgatgc cggagcgttc gagcgcgacc aatcccgcct
     3661 gggcctcgcc gctgccaccc atcgacttcg cttcgggatc accgaggagc atcccggacg
     3721 cgaccccgac cagaccgagc agcaccgcca ggctgaagac gatcgccgcc acgcggtggc
     3781 ggaccaccag gcgggcccag gaggcccagt ggcgttcggc gcgatcggtc cggcgcaagc
     3841 gccggcggtc ggcgcggggg ccgaacttgg ccagcacgac cggcagcagg gtcagcgcca
     3901 cggcgaccga gacgagcggg atcagcaggc cgccgtaggc catcgagcgc aggaagggga
     3961 tcggcaggac caccgcggcg agcagcccga tcgccacggt cgtgccgctg aagaggacgg
     4021 cgtggccggc ggtccgcgag gcggccagca cggcctcctc gttcccgaga ccgcgatcgc
     4081 gttcctcgcg ccagcgcatc accacgatca gggcgtagtc gatcgccagc ccgagcccga
     4141 tcagcgagca gaggaagagc acgatgaagg agacgtcggt gacggcggtg aggccgagca
     4201 cggcgagcag ggtcaccggg atcgcgatga ccgccatcaa cagcggcacc agcgccaggg
     4261 ccgaagcgaa gacgaagacc agcacgacca gggcggcgac gccggcgatc agcgtctcgt
     4321 tgaggaagga cgagtcgccg ccttcctctt cgggcgactc gccttcgagc gcggcgcgac
     4381 cggtgagcag cacgtcggag ccgccaacct cggacttggc cagcgccccg cgcgcggctt
     4441 cgacggtctg cggcgagatc tcggcgccgg ggccctcgcc ctgttcgatc ggcgggtagg
     4501 caaggacgaa ggtggtgcgg ccgtcgtcgg agacgtaggc ggcttcgccg gtcgagccgt
     4561 aggaggcgac gcgggctccg ggcaacgccg cggtgagctt gcgctcggcc tgacgcaggt
     4621 cagaacggac ccccggatcg cgcacgttct cacccgcggg cagggtggtc accgcgacca
     4681 gcggcgtcgc ttcgccgccg ccaccgaagc gctcggcgat ttcctggttg acggcgtagg
     4741 ccgggctcga gggcatcgag aactgttcgt cgagccgatc ggtcaccttc gaggagccgt
     4801 agaagccggc gacggtgagg acgatccaga agatcgcgac caggccctta tgggcgagga
     4861 cccagcgggt cacggtttcg agcccgcgac cggtgcgggc ccccgacggg gcggaaaggc
     4921 gtggttggct catggcgctg aacctaggcc cgaccggccg gcgcgaaaac gtcccgggga
     4981 aggagattgg cgctcctcct ctgggaggag cggggctcac cccagaggag gtttcgaccc
     5041 ccggcgggcg gccatagtct ctgtcccgtg ctcgggctcg atcgatcctc gcagcggttg
     5101 cgcgccctga ccgagcacgg accgaacgcg gtggggagct cgccggcgcc ggggtcgtcc
     5161 cggtacaggc tcctcgacgc gggcgtcgtc ctcgcggtgc tcgcggtctc gctcgtccag
     5221 ctcgccagcc acgttttcgt cgacgacgac gtggccggcg agctggacga ccccgacgtt
     5281 ctcggcgtcg tcctggtcct cctgacctcg ctgcccttgt tctggcggcg gcggcatccc
     5341 tgggcggtga tggcgatcgc cggcggtggc ggcatcgccc tggtcgcctg cggctactcg
     5401 gtgaatgtgc cggcggcgct tttcgtcgcc ctgtacacgt tcgccgccag cggtcgcggc
     5461 gcgacctggc ccccgatcgc ctttgccggg ttgctcttcg tcgcccaggt cgtggtcgag
     5521 atcgccgtgc tctcgctttc gatcgaggac tacctgattc ccgccctgct gctggccggc
     5581 gcctgggtct tcggcgagcg ccgccacacc gccgcgctgc gcgaggccga ggaacacgaa
     5641 cgccgcgagc gcgaggaacg gctctcgatc gccgaagagc gcacccggat cgcccgcgag
     5701 ctgcacgact ccgccgggca cgcgatcaac acgatcctcg tccaggcggg cgccgcccgg
     5761 gtgctgcgcg agcgcgatcc cgagcgctcg caggaagcga tcgaagcaat cgagcgcctc
     5821 gccctggaga cgatcgagga catcgaccgg atcgtcggtt cgctgcgcgc cgagggtccg
     5881 gccgagcggg cgccgctgcc cggcatcgag cggatcccgg acctcgtcgc cgagcagcgc
     5941 cgggccgggc tcgaggtcga gctgcgcgag ctggccgacg attggcgcaa gcccccaccg
     6001 gcggtcgacc gcgccgcgta ccggatcgcc caggaggcgc tgaccaacgc cgcccgccac
     6061 ggcagcggac gcgtcgagct gaccctccac caacacgtcg accggctcga gctgatcgtc
     6121 gtcaacccga tcgccgcggc gccggcgccg gcgcgaccgg gcggcggtgg cctcggcctg
     6181 atcgggatgc gggagcgggc gcagctgctc ggcggcttcc tcgaagcggg ccccgtggat
     6241 ggccgcttcc gcgtccgcgc ggtctttccc tacagttgga tccgggagtg agaccgaacc
     6301 ccgacagcgg ccgcgagcgt ggcccgatcc gggtgatgat cgtcgacgac gacgagctga
     6361 tgcgaaccgg gctgcgcgcg gtcctctcca ccgacgaccg gatcgaggtc gtcgaggagg
     6421 ccggcgacgg cgaacaagcg gtgcgccggg ccgggcgcac tcagcccgag gtcgtgctga
     6481 tggacgtgcg gatgcccggc caggacggga tcgccgccac cgccgatctg ctcgcggtcg
     6541 cgcccgaggc caaggtcgtg atcctcacca ccttcgagga ggacgagtac atccacggtg
     6601 cgctgcgcgc cggcgccagc ggcttcctgc tgaagcgcac ctcgccggag gacctgaccg
     6661 ccgcggtcca ccgggtcagc gccggcgagg cgctgctctc gccctcggtg acccgccggg
     6721 tgatcgacca gatggtcgcg cgccccctgc cccgggcggg cgacgatccc cgcctggccg
     6781 agctgaccgc acgcgagcga gaggtgctgg agctgatcgc ccgcggcctc tccaaccgcg
     6841 agatcgccgc ccagctcgtg gtcgaggaga cgaccgtgaa gacgcacgtc aagcgggtgc
     6901 tggcgaagct cggcgtgcgc gaccgcgtgc acgccgtgat cgtcgcctac gaaaccggcg
     6961 tcgtcgcgcc gtaggccgat ccggcgaggc ttgcgaatcg ccgcgagggg tcctacggtg
     7021 gggcgatgcg agccttttta ctggtcccgg tagcggtgct gggtctgatg gccgcgagcc
     7081 cggtggtctc ggctgcggag acgccggggt tcgagctggc gaagaagggc aaggcatgta
     7141 agggcaccaa ggtcgcggtc aaacagagcg ggaagacgat ctgcaaggcg ttcggccagg
     7201 tcttcccgaa accgcgggcg aacgacctgc ggctcgccta cctccagcag accctgcggc
     7261 tcgacccggc ggagctgctc aagggcaaga aggccaagcg ggcccgcacc ctgcagagcg
     7321 gctttggcgc ggccggcaag cgggcccaga agaagctgct gggcctgctt ccgaaagcgc
     7381 tggcgttctt cgaccgcaag cgggccggcg cgcgcagctc gacgctcggt gcaggcgcga
     7441 cggcgtcggc gaactgcggc gtcggcgccg cgggggacag gggcagcctg ggcgggtcgt
     7501 cgacggtcgg gatgctcggc ggcaacgggc tcttcatcga aaccaacgcg ggcggcggcc
     7561 tgaggatccg cgtcaccttc ttctcctgcg gaggcgtcga cagcttccgc atccccgagt
     7621 gcccgaccgg cgaggggaac gtggtggccc agaccgcgac cggcgacttc tcggcgacga
     7681 ccgaagtgtt ccggggtggc gagttcgagt cccgcaacac cagcaggttc gaagacacct
     7741 ccaaggcgaa aggcaaggtc ggcaaggacg cgaagctgaa atacatcgaa gtcgagcacg
     7801 agcaggaggt cctgatcgtc gccagcgggg gattcgtggc ccggggcggg atgaaacgcg
     7861 aagtgaaaat cgacatgccc gccggcaaat acgaccccgc
//
LOCUS       DASRIG010000157         6298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_601543, whole genome shotgun sequence.
ACCESSION   DASRIG010000157 DASRIG010000000
VERSION     DASRIG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601543"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..836
                     /locus_tag="VFB52_04305"
     CDS             45..836
                     /locus_tag="VFB52_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO05586.1"
                     /translation="MTDLVLAERRGAVLVLALNRPDRLNAWNDALELEYFAHLDAAEA
                     DPGVRAIVLTGAGRGFCAGADMDVLDGATGEGGVPDEIVNRPRRRDLPLTLRKPLIGA
                     INGAAAGLGFVEALYCDVRFCVPKAKLTTAFVRRGLVAEYGVAWFLPRLVGTSRALDL
                     LLSGRVMLGEEAHRIGLVDHLCEPETLLDDAVAYAEDLTANCSPTSMAIIKAQVQRGL
                     DHSFEDNFAESERLIAESFDRSDATEGVESYLEKRPPAFPPLDAP"
     gene            complement(833..2461)
                     /locus_tag="VFB52_04310"
     CDS             complement(833..2461)
                     /locus_tag="VFB52_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05587.1"
                     /translation="MPRASSLSTWTTEIPANGVELFERGERERPDDAALLYFETALSF
                     RAAGGLAHALAAALREDLGLRAGDRVALMLQNVPQMAIAVHAVWLAGGIVTTLNPMYR
                     RAELEHQIGDAGAEIVFCLESLHEVVAEARATAGVRQVVTVSELDFLDAVPDALAGHA
                     RIECPDAVAIADLLAAHAGERVEPAAPAPGDPALLTYTSGTTGVSKGAINTHRAVVHN
                     AATMTRWYELGPGDVTVAMAPLFHITGLVCHLATARFSLTPLLLGYRFEPGELLRLIE
                     RWRGTYVIGPLTAFIAMLEHPDMATRDLSSLTKVASGGAPVNPATVERWERATGAYVH
                     NTYGLTESAAPSHLVPFGARSPVDPESGALSIGVPIYDTEARVVDIENGAELGPGEIG
                     EIVLRGPTVTPGYWQRPEETAHALREGWLHTGDVGKRDTEGWFYVVDRIKDMIVASGY
                     KVWPRDVEDVLFRHPAVREASVVGVPDDYKGEAVRAYVALRQGEDASEAELVEHCRAA
                     LAAFKAPREVVFVDEIPKTLSGKALRRELRARAD"
     gene            complement(2424..2918)
                     /locus_tag="VFB52_04315"
     CDS             complement(2424..2918)
                     /locus_tag="VFB52_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HZO05588.1"
                     /translation="MGENETAQDQLGLRPLKEITLDERVIVLDEIPWVPYTEGIFVKP
                     LRLNRRTGVWANITKVEGGMQINRHYHVNPVTGFVLEGSWYYAEKDWVAREGTFVWEP
                     PGDIHTLISNEEGTVTLFMLEGALLYVDEDDNVVGFDDVLAHMHKYHEFCRAEGIEPV
                     DLDY"
     gene            complement(3039..4085)
                     /locus_tag="VFB52_04320"
     CDS             complement(3039..4085)
                     /locus_tag="VFB52_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZO05589.1"
                     /translation="MSENGHSNGNGDGGGVATLTKADFQSDQETRWCPGCGDYAILNS
                     VQTLMPELGVPPEKTVFISGIGCAGRFVYYMDTYGMHGIHGRAPALATGIATSRPDLS
                     VWVVSGDGDSLSIGGNHLIHALRRNVPIKLLTFNNQIYGLTKGQYSPTSETGKVTKST
                     PFGSEDHPFNPLALALGADASFVARTIDVEKQHVRDTLRAAAEHPGAAFVEIYQNCNV
                     FNDGAFDAVRGKGGAHNQIRLEHGQPIRFGAENERGVIRSAAGGLEVVDVADVGEDAL
                     VVHDAHVTDPSHATALARLADSPTGPTPIGVFRDVKRDVYGSGRQDALRKAHAGSTLD
                     DVDELLRSGDTWVV"
     gene            complement(4082..5923)
                     /locus_tag="VFB52_04325"
     CDS             complement(4082..5923)
                     /locus_tag="VFB52_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZO05590.1"
                     /translation="MTTTSKKPETVIVRFAGDSGDGMQLVGDRFTAATAHVGNDLSTL
                     PDFPAEIRAPAGTPGGVSAFQIHFASRDILTPGDHPNTLVAMNPAALKTHLGALEAGG
                     TLIVNEDAFTANNLRKAGYGEVNPLEDGTVEDYQVLAVPMTTLTTRATEDVEGLKPAE
                     AQRTKNLFALGLISWMYGRPLEDTVSWLEAKFAKKPAIMAGNIAALKAGYNFADTIEI
                     TVTPTEVAPAPLDAGEYRNVNGANALAMGLVAASVRSGLQLYYAGYPITPASELLHQL
                     ARHDRFGVRTVQAEDELAAANMALGASFGGQLGVTATSGPGMDLKAETIALAAMVELP
                     MVIIDVQRSGPSTGLPTKTEQSDLMLAIHGRHGESPLPVLAAQTPSDCFRAAVEAASI
                     AIRYRTPVILLADTFLMNSSEPWRLPEAADLPEIDPEFTTEPNAGDEFLPYVRNERGA
                     RPWALPGTPGLQHRLGGLEKEHGSGKVSYDAENHALMTKLRAEKVAKVADDIPPLALD
                     ADEGAELLIIGWGSTFGVIGAAARRVRNRGYKVATAHLRHLDPFPHNTGEVVKAYPKV
                     LVPELNTGQLLSLLRDKFLVDAVGHNKVEGLPILAEELEQAILEQIG"
     gene            6049..>6298
                     /locus_tag="VFB52_04330"
     CDS             6049..>6298
                     /locus_tag="VFB52_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO05591.1"
                     /translation="MGSGRRAAVVGAGIGGLASAIALARAGWEVTVYEANPEHRPLGA
                     GLSLWPNGVRAMRWLGLGELVEESLHQAGGVRRADGRML"
BASE COUNT          842 a         2119 c         2341 g          996 t
ORIGIN      
        1 gaggtcggca ccttcttcga aggccgctga gttagcttgg tccgatgacc gatctcgtcc
       61 tcgccgagcg ccgcggcgcc gtcctcgtcc tcgccctcaa ccggcccgac cggctgaacg
      121 cctggaacga cgcgctcgag ctggagtact tcgctcacct cgacgccgcc gaagccgacc
      181 ccggggtgcg ggcgatcgtg ctgaccgggg ccggccgcgg cttctgcgcc ggcgccgaca
      241 tggacgtgct cgacggcgcg accggcgagg gtggcgtgcc cgacgagatc gtcaaccggc
      301 cgcggcggcg cgacctgccg ctgaccctgc gcaagccgct gatcggggcg atcaacggcg
      361 ccgccgccgg gctcggcttc gtcgaggcgc tctactgcga cgtccgcttc tgcgtgccga
      421 aggcgaaact gaccaccgcc ttcgtccgcc gcggcctcgt cgccgagtac ggggtcgcct
      481 ggttcctgcc gcggctggtc ggcaccagcc gtgccctcga cctgctgctc tccgggcgcg
      541 tgatgctcgg cgaggaagcg caccggatcg gcctcgtcga ccacctctgc gagcccgaga
      601 ccctgctcga cgacgcggtc gcctacgccg aggacctgac cgccaactgc tcgccgacct
      661 cgatggcgat catcaaagcc caggtccagc gcggcctcga ccacagcttc gaggacaact
      721 tcgccgaatc cgagcgcctg atcgccgagt ccttcgaccg ctccgacgcc accgagggcg
      781 tcgagagcta cctcgagaag cgccccccgg ccttcccgcc gctcgacgcg ccttagtcgg
      841 cccgcgcccg cagctcgcgg cgcagggcct tgccgctgag ggtcttcggg atctcgtcga
      901 cgaagacgac ctcgcgcggg gccttgaagg cggcgagggc ggcgcggcag tgctcgacca
      961 gctcggcctc gctcgcgtcc tcgccctggc gcagggcgac gtaggcgcgc acggcttcgc
     1021 ccttgtagtc gtcgggcacg ccgaccacgc tcgcctcgcg cacggccggg tggcggaaga
     1081 gcacatcctc gacgtcgcgc ggccagacct tgtagccgga ggcgacgatc atgtccttga
     1141 tccggtcgac gacgtagaac cagccctcgg tgtcgcgctt gccgacgtcg ccggtgtgca
     1201 gccagccctc gcgcagggcg tgcgcggtct cctccggccg ctgccagtag ccgggcgtca
     1261 ccgtgggccc gcgcaggacg atctcgccga tctctcccgg tcccagctcg gcgccgttct
     1321 cgatgtcgac gacccgcgcc tcggtgtcgt agatcggcac gccgatcgag agcgcgccgc
     1381 tctcggggtc gaccgggctg cgcgcgccga acgggaccag gtgactgggc gccgccgact
     1441 cggtcaggcc gtaggtgtta tggacgtagg cgccggtcgc ccgttcccag cgctcgaccg
     1501 tcgccgggtt gaccggggcg ccgccgctgg cgaccttggt cagcgaggag aggtcgcgcg
     1561 tggccatgtc cgggtgctcg agcatcgcga tgaaggcggt cagcgggccg atcacgtagg
     1621 tgccgcgcca gcgctcgatc agccgcagca gctcgcccgg ctcgaagcgg tagccgagca
     1681 gcagcggggt gaggctgaag cgggcggtgg cgaggtggca gacgagcccc gtgatgtgga
     1741 agagcggcgc catcgccacg gtaacgtcgc cgggcccgag ctcgtaccaa cgggtcatcg
     1801 tggcggcgtt gtggacgacc gcgcggtggg tgttgatcgc gcccttcgag accccggtgg
     1861 tgcccgaggt gtaggtgagc agcgccgggt cgccgggggc gggggccgcc ggctcgaccc
     1921 gctcgcccgc gtgggcggcg agcaggtcgg cgatcgcgac cgcatccggg cactcgatcc
     1981 gcgcgtggcc ggcgagcgcg tccgggaccg cgtcgaggaa gtcgagctcg gagaccgtga
     2041 ccacctggcg cacgccggct gttgcccgcg cttccgcaac gacctcgtgc agcgactcga
     2101 ggcagaagac gatctcggcg ccggcgtcgc cgatctggtg ctccagctcg gcccgccggt
     2161 acatcgggtt gagggtggtg acgatcccgc cggcgagcca gaccgcgtgc acggcgatcg
     2221 ccatctgcgg cacgttctgc agcatcagag cgacccggtc gccggcccgc agccccaggt
     2281 cctcgcgcag cgccgccgcc agcgcgtgcg ccaggccgcc cgccgcgcgg aagctcagcg
     2341 cggtctcgaa gtagaggagc gcggcgtcgt cggggcgctc gcgctcgccc cgctcgaaca
     2401 gctcgacgcc gttggccggg atctcagtag tccaggtcga caggctcgat gccctcggca
     2461 cggcagaact cgtggtactt gtgcatgtgg gcgagcacgt cgtcgaaccc gaccacgttg
     2521 tcgtcctcgt cgacgtagag cagcgcgccc tcgagcatga acagcgtgac cgtgccctcc
     2581 tcgttggaga tcagcgtgtg gatgtcgccg ggcggctccc agacgaaggt gccctcgcgc
     2641 gcgacccagt ccttctccgc gtagtaccag gatccctcga gcacgaaccc ggtcaccgga
     2701 ttgacgtggt agtggcggtt gatctgcatc ccgccttcga ctttggtgat gttcgcccag
     2761 acccccgtgc gccggttcag ccgcagcggt ttgacgaaga tcccctcggt gtaggggacc
     2821 cagggaatct cgtcgagcac gatcacccgc tcgtcgaggg tgatctcctt cagcgggcgc
     2881 aggccgagct ggtcctgcgc ggtctcgttt tctcccatgc gacgaccgta agcgaccacg
     2941 accgcgcagc gacgcgcttt ccggcgccgt gctggaggaa ccacaggcgc tcggcacatt
     3001 gaccacaacc tggtgtggtc aatgtcgcga ggggtttctc agacgaccca ggtgtcgccg
     3061 gagcggagca gctcgtcgac gtcgtcgagc gtcgacccgg cgtgggcctt gcgcagcgcg
     3121 tcctggcggc cggagccgta gacgtcgcgc ttgacgtcgc ggaagacgcc gatcggggtc
     3181 ggcccggtcg ggctgtcggc gagacgggcg agcgcggtgg cgtggctggg gtcggtgacg
     3241 tgggcgtcgt ggacgacgag cgcgtcctcg ccgacgtcgg cgacgtcgac cacctcgagc
     3301 ccgccggcgg cggagcggat gacgccgcgc tcgttctcgg cgccgaagcg gatcggctgg
     3361 ccgtgctcga ggcggatctg gttgtgggcg ccgcccttgc cgcggaccgc gtcgaaggcg
     3421 ccgtcgttga agacgttgca gttctggtag atctcgacga aggcggcgcc ggggtgctcg
     3481 gccgcggccc gcagggtgtc gcgcacgtgc tgcttctcga cgtcgatcgt gcgggcgacg
     3541 aaggaggcgt cggcgccgag cgccagcgcc agcgggttga acgggtggtc ctcagagccg
     3601 aacggggtcg acttggtgac cttgccggtc tccgaggtcg gcgagtactg gcccttggtc
     3661 agcccgtaga tctggttgtt gaaggtcagc agcttgatcg ggacgttgcg gcgcagcgcg
     3721 tggatcaggt ggttgccgcc gatcgagagc gagtcgccgt cgccggagac gacccagacc
     3781 gagaggtcgg ggcgcgaggt cgcgatcccg gtcgccagcg ccggcgcgcg gccgtggatg
     3841 ccgtgcatcc cgtaggtgtc catgtagtag acgaagcgcc cggcgcagcc gatcccggag
     3901 atgaagacgg ttttctccgg cggcacgccg agttcgggca tcagcgtctg gaccgagttg
     3961 aggatcgcgt agtcgccgca gcccgggcac cagcgggttt cctggtcgga ctggaagtcc
     4021 gccttggtca gggtggcgac gccgccgccg tccccgttgc cgttgctgtg tccgttctcg
     4081 ctcatccgat ctgctccaag attgcctgct ccagctcctc ggcgaggatc ggcagaccct
     4141 cgactttgtt gtgcccgacc gcgtcgacca ggaacttgtc gcgcagcagg ctgagcagct
     4201 gcccggtgtt cagctccggc accagcacct tcgggtaggc cttgacgacc tcgccggtgt
     4261 tgtgcgggaa ggggtcgagg tggcgcaggt gcgcggtcgc gaccttgtag ccgcggttgc
     4321 ggacgcggcg ggcggcagcg ccgatcacgc cgaaggtcga gccccaaccg atgatcagga
     4381 gctcggcgcc ctcgtcggcg tcgagcgcca gcggcgggat gtcgtcggcg accttggcga
     4441 ccttctcggc gcgcagcttg gtcatcagcg cgtggttctc ggcgtcgtag gagaccttgc
     4501 cggagccgtg ctccttctcg aggccgccga gccggtgctg caggccgggg gtgccgggca
     4561 gcgcccaggg ccgggcgccg cgctcgttgc gcacgtaggg caggaactcg tcgccggcgt
     4621 tcggctcggt ggtgaattcg ggatcgatct ccggcaggtc ggccgcctcc ggcaggcgcc
     4681 agggctcgga ggagttcatc aggaaggtgt cggcgagcag gatcaccggg gtccggtagc
     4741 ggatcgcgat cgaggcggct tcgaccgctg cccggaagca gtccgacggg gtctgcgccg
     4801 ccagcacggg cagcggcgac tcgccgtggc gaccgtggat cgccagcatc aggtcggact
     4861 gttcggtttt ggtcggcagc ccggtcgagg ggccggagcg ctggacgtcg atgatcacca
     4921 tcggcagctc gaccatcgcc gcgagcgcga tcgtctccgc cttcaggtcc atgcccgggc
     4981 cgctggtcgc ggtcaccccg agctggccgc cgaaggaggc gccgagcgcc atgttggcgg
     5041 ccgcgagctc gtcctcggcc tgcaccgtgc gcacgccgaa gcggtcgtgg cgggcgagct
     5101 ggtggagcag ttcggaggcc ggcgtgatcg gatagcccgc gtagtagagc tgcaggccgc
     5161 tgcgcacgga ggcggcgacc aggcccatcg ccagcgcgtt ggcgccgttg acgttgcggt
     5221 actcgccggc gtccagcggg gccggcgcga cttcggtcgg ggtgaccgtg atctcgatcg
     5281 tgtcggcgaa gttgtagccg gctttgaggg cggcgatgtt gccggccatg attgccggct
     5341 tcttggcgaa tttcgcttcc agccaagaga cggtgtcctc cagcggccgc ccgtacatcc
     5401 acgagatcag accgagcgcg aagaggttct tggtccgctg cgcctccgcc ggtttcagcc
     5461 cctcgacgtc ctcggtggcg cgcgtggtca gcgtcgtcat cggcaccgcc agcacctggt
     5521 agtcctcgac cgtgccgtcc tcgagcgggt tgacctcgcc gtagccggcc ttgcgcaggt
     5581 tgttggcggt gaaggcgtcc tcgttgacga tcagcgtgcc gcccgcctcg agcgcaccga
     5641 ggtgggtctt cagcgccgcc gggttcatcg cgaccagcgt gttcgggtgg tcgcccgggg
     5701 tgaggatgtc gcgcgaggcg aagtggatct ggaaggcgga gacgccgccc ggggtaccgg
     5761 cgggggcgcg gatctcggcc gggaagtccg gcagggtgga gaggtcgttg ccgacgtgcg
     5821 cggtggcggc ggtgaagcga tcgccgacca gctgcatgcc atcgccagaa tctccggcga
     5881 accggacgat gacggtctct ggcttcttcg aagtcgtggt cactgtggtg ccgcgcctcc
     5941 ctagggccgg cccaaacgga cggaacaata gggctgcgca cggcgggtcg caagtccttt
     6001 accaatgcga ggttggatga gtcgccagat cgtcattagg ttgggggcgt ggggagcggt
     6061 cgcagagctg cggtggtggg ggccgggatc ggcgggctgg cgagcgcgat cgcgctggcg
     6121 cgggccggct gggaggtcac tgtctatgag gctaaccccg agcaccgccc cctcggggca
     6181 gggctttcgt tatggccgaa cggagtgcgg gcgatgcgct ggctcgggct cggcgagctg
     6241 gtcgaagaat cgctgcacca ggcgggcggc gtgcgccggg ccgacgggcg gatgctgg
//
LOCUS       DASRIG010000158         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3740098, whole genome shotgun sequence.
ACCESSION   DASRIG010000158 DASRIG010000000
VERSION     DASRIG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3740098"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFB52_04335"
     CDS             <1..399
                     /locus_tag="VFB52_04335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO05592.1"
                     /translation="LALMILDLDDFKGINDRYGHPVGDAVLRDAAARAQSMLRSTDTL
                     ARIGGDEFAVIAPGARGENAQRMAESIRTAVSAHDPLSEGPSPTASIGWAVFPEDGED
                     FETLMHCADQRMMQLKSGRQAEVVSLLEAP"
     gene            complement(410..760)
                     /locus_tag="VFB52_04340"
     CDS             complement(410..760)
                     /locus_tag="VFB52_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HZO05593.1"
                     /translation="MGTAQIPDPTESVRHQLRPLFDQVVIKEIDSDRVRRSGLVVPPG
                     THEPPPEQGIVLAVGPGLDWWANAGVEMPVAPGDHVVFPAAAGVWVEVDEERLLVCRV
                     TQLLGVLESAALER"
     gene            890..1747
                     /locus_tag="VFB52_04345"
     CDS             890..1747
                     /locus_tag="VFB52_04345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZO05594.1"
                     /translation="MHQSTRLRLPWALALALVVSLFLVFAAGAARAANYVSLGDSYAA
                     GPLIPNQSLNPLGCLRSSNNYAHLAAPTIGLTHRDPSCSGAKTNHMTETQNVEPGPNP
                     PQFNSLDAETTVVTLTIGGNDIGFSEVAQSCITINPFSTPCKNKYAPGGKDQLQERIN
                     ATAPKVDAVLDGIKARSPLAKIYVVNYPAIFPETGSGCWPQMPIGFGDVPYLRQTEKS
                     LNAMLAAQAAANGAKLVDWYTASIGHDACKSTSNRWVEPLIPGTAAAPIHPNKAGMQG
                     GANALIAAL"
     gene            complement(1754..2581)
                     /locus_tag="VFB52_04350"
     CDS             complement(1754..2581)
                     /locus_tag="VFB52_04350"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05595.1"
                     /translation="MERGESKVWFITGASKGFGRIWAEAALARGDRVAATARDPRALD
                     GLVDRYGELVLPLQLNVDDKAADDAAVARAHEHFGRLDVLVNNAGYGLFGAIEEVSEE
                     QARQQLETNVFGALWVTKAALPIMREQGSGHILQVSSIGGVQAFPGIGLYHASKWALE
                     GFSQSLAGEVGPLGIHVTLIEPIGYETDWAGPSAVVADPLPAYEPIREVRKTSRVRNQ
                     RGTPEATAAAILAVVDSDDPPLRLFLGQWALDMIREEYANRLEVWERWQPVAASAHE"
     gene            2690..3736
                     /locus_tag="VFB52_04355"
     CDS             2690..3736
                     /locus_tag="VFB52_04355"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZO05596.1"
                     /translation="MPSSGHSSVARIAWIAVLALVVFVVWLLVNQTGGGGIGFFYAIP
                     IGLATWWFGLRAGVVTALACLALYVIGALIEPVTNVGVALAIRALAFAGVVGVVSILR
                     ERLLVLEQEVEELEDIRAALTPTSLPDLPDVEAGAAFAPSDHGVSGDFYLLTNGPDGS
                     TVAVVGDVVGHGPRAARVATFIRARLAAFAANTSEPAQILELANGALLERPDREDRIV
                     SAICLRYEPDGSTLSWAVAGHPPPLRLPDLAELRHQGPTLLLGIEPRLELEATTIPLG
                     PAEGVLVYTDGATDVRRGRNLLGREGLARLLTPHAHLPSHRLAREAHRAVLEWTDAPL
                     GDDLCLVVLRPKSS"
     gene            complement(3733..>4173)
                     /locus_tag="VFB52_04360"
     CDS             complement(3733..>4173)
                     /locus_tag="VFB52_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO05597.1"
                     /translation="AEVADAAAAARLLHDFNAEYDEPSPGVAALTETLARLIEAGEVT
                     VLLAGDGPDGISLMRLRPALWSAGLEAYLQELYVVPDLRGQGIGRALLEETMAVARAA
                     GAAGIDLNTGETDTEARGLYESAGFTNREGGPDGPAMLFYEREL"
BASE COUNT          620 a         1584 c         1355 g          614 t
ORIGIN      
        1 ttggcgctga tgatcctcga cctcgacgac ttcaagggaa tcaacgaccg ctacggccac
       61 ccggtcggcg acgcggttct ccgcgatgcc gccgcccgcg cccagtcgat gctgcgctcg
      121 accgacacgc tggcccggat cggcggcgac gagttcgcgg tgatcgcgcc cggcgcccgc
      181 ggcgagaacg cccagcggat ggctgaatcg atccgcaccg cggtcagcgc ccacgatccc
      241 ctctccgagg gaccctcgcc gaccgcctcg atcggctggg cggtcttccc cgaggacggc
      301 gaagacttcg agacgctgat gcactgcgcc gaccagcgga tgatgcagct gaagtccggc
      361 cgccaggccg aggtcgtctc gctgctcgaa gctccctaga gcctcggcgt catcgctcga
      421 gcgcagccga ttcgagcacg ccgagcagct gggtgacgcg gcagaccagc aggcgctcct
      481 cgtcgacctc gacccagacg ccggcggcgg ccgggaagac gacgtggtcg ccgggcgcga
      541 ccggcatctc caccccggcg ttcgcccacc agtcgaggcc cgggccgacg gcgagcacga
      601 tgccctgctc gggcggcggc tcgtgggtgc ccggcgggac gacgaggccc gagcggcgca
      661 cccggtccga atcgatctcc ttgatcacga cctggtcgaa cagcggtcgt agctggtggc
      721 ggaccgactc ggtcgggtcg gggatctgcg cggtgcccac ggacccaatt ctggcgcatc
      781 cgcaggattt gccggctatc cggccaccgg ctttgcgcct ggtttagaac caaagtaaat
      841 tgagcaatag ggaggcaggg gacttcccac tacaaggagg cgtcacaaaa tgcaccagtc
      901 caccaggctg cggctgccct gggcccttgc cctcgcactc gtggtatccc tgttcctcgt
      961 cttcgcggca ggggccgcgc gggcggcgaa ctacgtatcc ctgggcgatt cctacgcggc
     1021 cgggccgctg atcccgaacc agtcgctgaa cccgctcggc tgtctgcgct cgagcaacaa
     1081 ctacgcgcac ctggcggcgc cgacgatcgg gctcacgcac cgtgacccga gctgcagcgg
     1141 cgccaagacc aaccacatga ccgaaaccca gaacgtcgaa ccggggccga acccgcccca
     1201 gttcaactcg ctggacgcgg aaacgacggt ggtcacgctg acgatcggcg gcaacgacat
     1261 cggcttctcc gaagtcgcgc agagctgcat cacgatcaac ccgttcagca ccccctgcaa
     1321 aaacaaatac gcgccagggg gcaaagacca gctgcaggaa cggatcaacg cgaccgcacc
     1381 gaaagtcgac gcggtcctcg acgggatcaa agcccgctca ccgctggcga aaatctacgt
     1441 cgtcaactac ccggcgatct tcccggagac cgggtcgggc tgctggccac agatgccgat
     1501 cgggttcggc gacgtgccct acctgcgcca gaccgagaaa tcgctgaacg cgatgctggc
     1561 cgcgcaggca gcggccaacg gggcgaaact ggtcgactgg tacaccgcca gcatcggcca
     1621 cgacgcctgc aaatcgacct cgaaccgttg ggtcgaaccg ctgatcccgg gcaccgccgc
     1681 ggcgcccatc catcccaaca aagccgggat gcagggcggc gccaacgcgc tgatcgccgc
     1741 gctctaaccc gccctactcg tgagcgctgg cggccaccgg ctgccagcgc tcccatacct
     1801 ccagccggtt tgcgtactcc tcgcggatca tgtccagcgc ccactggccg aggaagaggc
     1861 gcagcggcgg gtcgtcggag tcgaccacgg cgaggatcgc cgcggcggtc gcctccgggg
     1921 tgccgcgctg gttgcgcacg cggctggttt tgcgcacctc gcggatcggc tcgtaggcgg
     1981 gcagcgggtc ggcaactacc gcggaggggc cggcccagtc ggtttcgtag ccgatcggct
     2041 cgatcagggt gacgtggatg ccgagcggcc cgacctcgcc ggcaagcgac tggctgaacc
     2101 cctccagcgc ccacttcgag gcgtggtaga ggccgatccc ggggaacgcc tgcacgccgc
     2161 cgatcgagga gacctggagg atgtggccgg agccctgctc gcgcatgatc ggcagcgccg
     2221 ccttggtcac ccagagcgcg ccgaagacgt tggtctcgag ctgctggcgg gcctgctcct
     2281 cgctgacctc ctcgatcgcc ccgaagaggc cgtagccggc gttgttgacg agcacgtcga
     2341 ggcggccgaa gtgctcgtgg gcgcgcgcga ccgcggcgtc gtccgcagct ttgtcgtcga
     2401 cgttcagctg cagcggcaga accagctcgc cgtagcgatc gaccaggccg tcgagcgccc
     2461 gcgggtcgcg cgccgtcgcc gccacccggt cgccgcgggc cagcgcggct tccgcccaga
     2521 tccgcccgaa gcccttcgac gccccggtga tgaaccagac cttcgactcg ccgcgctcca
     2581 tatctggacc ctcctcgttc gcctgtcgcc tgttgcacgc ccagcgtagt gaacgccttt
     2641 cgcgggccag aactcgtcgc ggcatcgacg ccccgatagg ctgatcttcg tgccgagttc
     2701 aggccactca agcgtcgctc gcatcgcttg gatcgcagtc cttgcgctgg tcgtgttcgt
     2761 cgtctggctg ctcgtcaacc agaccggcgg cggcgggatc ggcttcttct acgcgatccc
     2821 gatcgggctt gcgacctggt ggttcgggct gcgcgcgggt gtcgtcacgg cgctcgcctg
     2881 ccttgctctc tacgtgatcg gcgcgctgat cgaaccggtc accaacgtcg gcgtcgcgtt
     2941 ggcgatccgg gcgctcgcct tcgccggagt ggtcggcgtc gtctcgatcc tgcgcgagcg
     3001 cctgctcgtg ctcgagcagg aggtcgagga gctggaggac atccgcgccg cgctcactcc
     3061 gaccagcctg cccgacctcc ccgacgtcga ggccggggcc gcattcgccc cctccgacca
     3121 cggcgtttcc ggcgacttct acctgctcac caacggtccc gacggctcga ccgtcgcggt
     3181 cgtcggcgac gtcgtcgggc acggcccgcg cgccgcccgc gtcgccacct tcatccgcgc
     3241 ccggctcgcc gcctttgccg ccaacaccag cgagccggcc cagatcctcg agctcgccaa
     3301 cggcgccctg ctcgagcggc ccgaccgcga ggaccggatc gtcagcgcga tttgcttgcg
     3361 ctacgagccc gacggctcga ccctctcctg ggcggtcgcc ggccacccac cgcccttgcg
     3421 cctccccgac ctcgccgagc tgcgccacca gggcccgacc ctgctgctcg ggatcgagcc
     3481 gcgcctggag ttggaggcga ccacgattcc gctcggcccg gccgagggcg tgctcgtcta
     3541 caccgacggc gccaccgacg tgcgccgcgg ccgcaacctg ctcggccgcg agggtctcgc
     3601 ccgcctgctt acaccgcacg cgcacctgcc gtcccaccgc ctcgcccgcg aggcccaccg
     3661 cgccgtgctc gagtggaccg acgccccgct cggtgacgac ctctgcctcg tcgtcctgcg
     3721 cccgaagtcg agctagagct cgcgctcgta gaacagcatc gccgggccgt ccggcccacc
     3781 ttcgcggttg gtgaagccgg cgctctcgta tagcccccgc gcttccgtgt cggtttcgcc
     3841 ggtgttgagg tcgatcccgg ccgcgccggc ggcgcgggcc accgccatcg tctcctccag
     3901 cagcgcccgc ccgatcccct gcccacgcag atccgggacc acgtagagct cctgcaaata
     3961 cgcttccagc ccggcgctcc agagagccgg gcgcagccgc atcagggaga tcccgtccgg
     4021 accgtcgccg gccagcagca ccgtcacctc cccggcctcg atcagccgcg ccagcgtctc
     4081 ggtcagcgcc gcgacccccg gcgacggttc gtcgtactcg gcgttgaagt cgtgcagcag
     4141 ccgcgccgcc gcggccgcgt ccgcgacctc ggc
//
LOCUS       DASRIG010000159         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3471963, whole genome shotgun sequence.
ACCESSION   DASRIG010000159 DASRIG010000000
VERSION     DASRIG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471963"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFB52_04365"
     CDS             complement(<1..434)
                     /locus_tag="VFB52_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05598.1"
                     /translation="MSRDAPTVVVTAVGAAEGARGAAAALACADADPDRATLLVDLDG
                     RPPRPTLLASTAARELEDRLAAHLPRTRVAARGQVCHLAAPADDDGFAAAAAAVTVAR
                     GGLAVIHAPPPALQALLASADAGEGPHATGVLLRADVPVDRAL"
     gene            complement(431..763)
                     /locus_tag="VFB52_04370"
     CDS             complement(431..763)
                     /locus_tag="VFB52_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZO05599.1"
                     /translation="MADSSGQASVELVAALPLLVVAALVTLQLLAAGYALTLADGAAE
                     AGALALAAGESAPEAARAALPGWAKDDVEVRVEGGRVTVRLRPPSPFGALSERLEVSS
                     AASARPAR"
     gene            complement(756..1121)
                     /locus_tag="VFB52_04375"
     CDS             complement(756..1121)
                     /locus_tag="VFB52_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05600.1"
                     /translation="MPPAADSRRHGEDGTASVELIATIPFLLFVLAVAVQATLAGHAL
                     WSAGVAARAGARAALVGGDIDAVARRALPPSLRRGSKVEEGEGDESGAVSVRVRVPSL
                     LPLPTFQVGASSGLEPGDG"
     gene            complement(1208..1561)
                     /locus_tag="VFB52_04380"
     CDS             complement(1208..1561)
                     /locus_tag="VFB52_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05601.1"
                     /translation="MRKVRNVAIIMLLALAAAFAPNGGNVVDAILTAITMAFLVGISW
                     MLYVLSRENQLTLATLTDGRRWIFYGGFALIVLLIAGASKLFDTGGGTVLWIILLVAA
                     VLAIYRVWTDANTYS"
     gene            complement(1579..1821)
                     /locus_tag="VFB52_04385"
     CDS             complement(1579..1821)
                     /locus_tag="VFB52_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05602.1"
                     /translation="MIFATLIEGKEMLETVAASVVAGIGVTTAFSVAIWGVGRFTEMN
                     NSERPVAAAGAAAVTTAALAFVGAALVFGIVVMVGN"
     gene            complement(1818..2921)
                     /locus_tag="VFB52_04390"
                     /pseudo
     CDS             complement(1818..2921)
                     /locus_tag="VFB52_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            complement(2938..3558)
                     /locus_tag="VFB52_04395"
     CDS             complement(2938..3558)
                     /locus_tag="VFB52_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZO05603.1"
                     /translation="MARFSLLPRDRTFFDLFIEAGQNNVHASRLLDQMMNSWPEEEGL
                     LEKIVEAEHEGDRITHEIVKRLNSTFVTPIDREDIYGLATQMDDIADFIEEAADFLGL
                     YQIEAPMEQATALTKVLVASCEQLAQGLEHLPDFKDLDKYWIEIHRLENEGDRISRDA
                     VASLFSNGIDPMVVIRWKDMFAVLEEAIDATETAAQILEGIVIKNS"
     gene            complement(3598..>5029)
                     /gene="ppk1"
                     /locus_tag="VFB52_04400"
     CDS             complement(3598..>5029)
                     /gene="ppk1"
                     /locus_tag="VFB52_04400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZO05604.1"
                     /translation="DHGYFRVTRDADFTVSDEADDLLQAVQDEIRRRRFGEVVRLEIA
                     AGMNPKLRQQLIGALRLEDREVYDVDGLIDLGDVGRIADVSGHAELRYSPWSPVTQPR
                     LQGEDDEPRDMFAEIRRQDVIVHHPYDSFASSVERFVAQAVADPDVLAIKQTVYRTSD
                     DSPLVPGLIRASERGKQAVCMVELKARFDEEANIHWAKSLEEAGVHVVYGIPGLKTHV
                     KAILVVRREGEKVREYVHIGTGNYHPKTARLYTDFGLFTADPDIGADVAEMFNFLTGY
                     GRPAVYRKVLVSPTTMRDRIVEEIERTVAAHEAGEDARIRMKMNSLVDAGCIRALYKA
                     SIAGVKVDLNVRGICCLRPGVPGVSENIRVVSIVGRFLEHSRVYAFQRGSEDRVLMGS
                     ADLMPRNLDSRVELVAPVEDEELKGEMLDVLDRCFADNTNAWELTEDGRWSREVTLGE
                     GRRNVQEELRERHAARAAEHLAASAS"
BASE COUNT          788 a         1788 c         1741 g          712 t
ORIGIN      
        1 agggctcggt cgacgggtac gtcggcgcgc aggaggacgc cggtggcatg cgggccctcc
       61 cccgcgtcag cggaggcgag cagcgcctgc agcgcggggg gaggtgcatg gatcacggcg
      121 agcccgcccc gggcgacggt caccgcggcg gccgcagcgg cgaaaccgtc atcatcggct
      181 ggcgcggcga ggtggcagac ctggccgcgg gcggcgacgc gggtgcgcgg taggtgcgcg
      241 gctagccggt cctccagttc gcgggccgcg gtcgaggcga gcagcgtcgg gcgcggcggg
      301 cggccatcga ggtcgacgag gagcgtggcg cggtcggggt cggcatcggc gcaggcgagc
      361 gcggcggcag cgccgcgggc gccctcggcc gcgccgaccg cggtgacgac gacggtcggg
      421 gcatccctgc tcatcgggcc ggcctcgcgc tcgcggcgct gctcacttcg agccgctcgg
      481 agagcgcgcc gaacggcgac ggcgggcgca gccgcacggt caccctgccg ccctcgaccc
      541 ggacctcgac gtcgtccttc gcccagcccg gcagcgccgc ccgcgcggcc tccggcgccg
      601 actccccggc ggcaagtgcc agcgcgccgg cctcggccgc gccatcggcc agggtgagcg
      661 cgtagccggc ggcgagcagc tgcagggtga cgagcgcagc gacgacgagc agcggcagtg
      721 ccgcgactag ctcaacactc gcctgtcccg aggaatcagc catcgccagg ctccagaccg
      781 gagctggcgc cgacctggaa ggtcggcagc ggcagcaggc tcggtacgcg aacgcgcacc
      841 gagaccgccc cgctctcgtc gccctccccc tcctcaacct tcgacccccg ccgcagcgac
      901 ggcggcagcg cgcggcgagc caccgcatct atatcgcccc cgaccagcgc cgctcgcgcc
      961 ccggcccgag cggcgacgcc cgccgaccag agcgcgtgac cggcgagcgt cgcctggacc
     1021 gccaccgcaa gcacgaacag caggaacggg atcgtcgcga tcaactcgac cgaggcggtc
     1081 ccgtcctccc cgtgacgtct gctgtcggcg gccggtggca ccggccaacc ctcaccgcga
     1141 tcccatcaac cgtggcgcgc agttcgcaaa atctctgcgc cggcttcggc gcggaagtga
     1201 ggctcgccta gctgtaggtg ttcgcgtcag tccagacgcg gtagatcgcc agcaccgcgg
     1261 cgaccaagag gatgatccag agcaccgtgc caccgccggt gtcgaacagt ttcgaggccc
     1321 ccgcgatcag cagcacgatc agcgcgaagc cgccgtagaa gatccagcgc cgcccgtcgg
     1381 tgagcgttgc cagcgtcagc tggttttcgc ggctgagcac gtagagcatc cagctgatcc
     1441 caaccaggaa ggccatcgtg atcgcggtca ggatcgcgtc gacgacattg cccccgttgg
     1501 gggcgaaggc ggccgccaga gccagcagca tgatgatcgc gacgttccga acctttcgca
     1561 cggttccagt ctacgccgct agttaccgac catcaccacg attccgaaga cgagcgcggc
     1621 gccaacgaag gcgagggcgg ccgtggtgac cgccgcggcg cccgccgccg ccactggccg
     1681 ttcgctgttg ttcatctcgg taaaccgccc gaccccccag atcgcaaccg agaacgcggt
     1741 ggtgacgccg atccccgcga ccaccgaggc ggccacggtc tccagcatct ccttgccctc
     1801 gatcagggtg gcgaagatca tgcgcccgaa gccggcaccg gcgcgccgcg ctgcgcccgc
     1861 cgcgcgaacg cagcgatgac cagcgagagc gccatcaggg tcaccaacac ggggccaagc
     1921 gcccccgagc cgaagatcgc cgtgaagccg tagaccgccg cgccgatcgc cgccgccgcc
     1981 ggcagggtca gagcccaggc ggtgaggatg ttgccggcga cgccccagcg caccgccgag
     2041 aggcgtttcg ccgcccccga gcccatcacg ccgccgttga tcacgtgcgt ggtcgagagc
     2101 gggaagccga agtgggtcga ggcgaggatc acggcggcgc cggccccctg ggccgcgaag
     2161 ccctgcgcgg cgtccatctt gatgatccgc gtgccagtcg ttttgatgat ccgccagccg
     2221 ccgctgtaag tgccgagcgc gatcgccgcc gcggcgctga agatcaccca gaacggcggg
     2281 tcggacccgg ccccgagctc gccgttggcg atcagggcga gggtgatgat ccccatcgtc
     2341 ttctgcgcgt cgttggtccc gtgggccaac gccaagagcc cgccggagac aacctggccg
     2401 tgcttgaagc cgcggctgac gatccccggc cgctgcttgc cgacgatccg gtagacgatc
     2461 ccaatcgaga gcccggcgac ggtgaaggca agcaccggcg cgaccaccgc cgggaccagg
     2521 accttgccga ccagcccttc ggcgatcacc gcgtcgaacc cgaccgcggc cacggccgag
     2581 ccgacgacgc cgccgatcag cgcgtgcgag gagctcgacg gcagcccgaa ccaccaggtg
     2641 atcaggttcc aggcgatcgc cccgatcaac ccggcgaaga tgatcgtcgg cgtgatcacg
     2701 tcggcgttga cgacgtcgtt ggcgaccgtc gcggcgacct agatcgagat gaaggcaccg
     2761 acgaagttga gcagcgccgc catcccgatc gccacctgcg gccgcgccgc cccggtcgag
     2821 atcgaagtgg cgacgacgtt ggcggtgtcg tggaacccgt tggtgaagtc gaacgccagc
     2881 gccgtgcaga tgacgatgac gaggatgacg tcggagctca ctgcgtccag gccaccatca
     2941 cgagttcttg atcacgatgc cctcgaggat ctgcgccgcg gtctcagtcg cgtcgatcgc
     3001 ctcctcgagc acggcgaaca tgtccttcca gcggatcacc accatcgggt cgatcccgtt
     3061 ggagaagagc gaggcgaccg catcacggga aatccggtcc ccctcgttct cgagccggtg
     3121 gatctcgatc cagtatttgt cgaggtcctt gaagtcgggc aggtgctcca gcccctgggc
     3181 cagctgctca cacgaggcta cgagcacctt ggtcagcgcc gtcgcctgct ccatcggcgc
     3241 ctcgatctgg tagagcccga ggaagtcggc cgcctcctcg atgaagtcgg cgatgtcgtc
     3301 catctgcgta gccagcccgt agatgtcctc gcggtcgatc ggggtgacga aggtcgagtt
     3361 cagccgtttg acgatctcgt gggtgatccg gtcgccctcg tgctcggcct cgacgatctt
     3421 ctccagcagc ccttcctcct cgggccagct attcatcatc tggtcgagca ggcgggaggc
     3481 gtgcacgttg ttctggcccg cttcgatgaa gaggtcgaag aacgttcgat cgcgaggtag
     3541 aagcgaaaaa cgcgccactg ggcgccatcc tagtgacggc ggaggcgccg gggggcgtca
     3601 gctcgcggaa gctgcgaggt gctcggccgc gcgcgccgcg tggcgctcgc gcagctcctc
     3661 ctgcacgttg cgccgcccct cgcccagcgt gacctcgcgc gaccagcgcc cgtcctcggt
     3721 cagctcccag gcgttggtgt tgtcggcgaa gcagcgatcg agcacgtcga gcatctcccc
     3781 tttcagctcc tcgtcctcga ccggggcgac cagctcgacc cggctgtcga ggttgcgcgg
     3841 catcaggtcg gcggagccca tcagcacacg gtcctcggac ccgcgctgga acgcgtagac
     3901 gcgcgagtgc tcgaggaagc gcccgacgat cgagaccacg cggatgttct cggagacccc
     3961 cggtacaccc ggccgcaggc agcagatccc gcgcacgttc aggtccacct tcacgcccgc
     4021 gatcgaggcc ttgtagagcg cccggatgca gccggcgtcg accagcgagt tcatcttcat
     4081 ccggatccgc gcatcctcgc cggcttcgtg ggcggcgacc gtgcgctcga tctcctcaac
     4141 gatccggtcg cgcatcgtcg tcggcgagac cagcaccttg cggtagaccg ccggccggcc
     4201 gtagccggtg aggaagttga acatctcggc gacgtcggcg ccgatgtcgg ggtcggcggt
     4261 gaagagaccg aagtcggtgt acaggcgggc ggtcttcggg tggtagttgc cggtcccgat
     4321 gtggacgtac tcgcgcacct tctcgccctc gcggcggacc accaggatcg ccttcacgtg
     4381 cgtcttcagc ccggggatgc cgtagacgac gtgcacgccc gcctcctcca gggacttcgc
     4441 ccagtggatg ttggcctcct cgtcgaaacg ggctttcagc tcgaccatgc agaccgcctg
     4501 cttgccgcgt tcggaggcgc ggatcagacc cggcaccagc ggcgagtcgt cgctggtccg
     4561 gtacaccgtc tgcttgatcg cgagcacgtc ggggtcggcg accgcctggg cgacgaagcg
     4621 ctcgaccgag ctggcgaagg agtcgtaggg gtggtggacg atcacgtcct ggcggcggat
     4681 ctcggcgaac atgtcgcgcg gctcgtcgtc ctcaccctgc aggcgcggtt gggtgaccgg
     4741 cgaccagggc gagtagcgca gctcggcgtg acccgagacg tcggcgatgc ggccgacgtc
     4801 accgaggtcg atcagcccgt cgacgtcgta gacctcgcgg tcttcgagcc gcagggcgcc
     4861 gatcagctgc tggcgcagct tcgggttcat cccggcggcg atctcgaggc gcacgacctc
     4921 gccgaagcgg cggcggcgga tctcgtcctg gaccgcctgc aggaggtcgt cggcctcgtc
     4981 ggagacggtg aagtcggcgt cgcgggtgac ccggaagtag ccgtgatcg
//
LOCUS       DASRIG010000160         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2153908, whole genome shotgun sequence.
ACCESSION   DASRIG010000160 DASRIG010000000
VERSION     DASRIG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2153908"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..541)
                     /locus_tag="VFB52_04405"
     CDS             complement(122..541)
                     /locus_tag="VFB52_04405"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO05605.1"
                     /translation="MRPVEQRISIAAPPAAVWAIAGDFGAANWISSIDAARMEGDLRY
                     AELDRGRGTVVERLVFHSDAERRFEYELAEEGASMRAFRGRFEVSGEGDSATVLWRGE
                     LTAVEAADEERLAAGVEDTYRRSLESLRTLVLDGQGA"
     gene            complement(538..1086)
                     /locus_tag="VFB52_04410"
     CDS             complement(538..1086)
                     /locus_tag="VFB52_04410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZO05606.1"
                     /translation="MLAMEERMTRRPPTSVQYREVIGHFASGVTVVTTAVGERSFGTT
                     ANAVTSVSAEPPMLLVCLNRDSETGKAIDQSGYFVVNILTEDQEHLAGHFATKDAGKF
                     DVAPFGHEEPPKLRSHERPLLEDALAHIECQVTERVTSATHIIFIGLVEVAEAGEGSP
                     LAYYRGSFGRLEVIPKERGRHG"
     gene            complement(1196..1801)
                     /locus_tag="VFB52_04415"
     CDS             complement(1196..1801)
                     /locus_tag="VFB52_04415"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05607.1"
                     /translation="MIQAAVETFHDKGYSATSIQDVADRVGVLKGSLYHYIDSKEDLL
                     ARIFEESDRQSFALMEEMEALDVPAVDRLREFARLWSLWYLENVERASLYFNEWKHLT
                     GERLAAVTQTRHEYEARVERMIRACKADGGADPDLDTHYACFFILSAINGLPIWYRRG
                     GPDSAEHISEVYADMIVATVTGSKGHKRPRAKKRKAKAKAA"
     gene            complement(1996..2721)
                     /locus_tag="VFB52_04420"
     CDS             complement(1996..2721)
                     /locus_tag="VFB52_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05608.1"
                     /translation="MSGLAVVTGAGTAGGLGAGIAARLAAAGHELLLVDLDPAVDARA
                     AELERDGASARAVVADLTAAEGAAAVATTAAESGAVALLVNNAGITRDGRATKLSEND
                     FAAVIAVNLVAAIRLAMALEPLYAEGAAVVNMSSRASLGNFGQANYVSSKSGLVGATR
                     ALALQWAPRVRVNAVAPGLIDTPMTAAMPAEVLEKLVGRIPAGRIGSPDDVAELVAFL
                     ASPAAGYVTGQVVLACGGRSLAA"
     gene            complement(2718..3086)
                     /locus_tag="VFB52_04425"
     CDS             complement(2718..3086)
                     /locus_tag="VFB52_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05609.1"
                     /translation="MAEPLILAQPPAADLDAITPLLTAAFATARDAVIAERPVVVLLD
                     DRDLLGQREVADAALATGLLGLVRALALEGAKPGWRVNAVAHRDEEEAARGAAEWLAG
                     SALSGQLIRVGAAHLGKVSP"
     gene            complement(3088..4281)
                     /locus_tag="VFB52_04430"
     CDS             complement(3088..4281)
                     /locus_tag="VFB52_04430"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZO05610.1"
                     /translation="MSAAVVVDALRTPMGRYGGSLAGVRPDDLAAQALAALVERNGVD
                     PAELDDVYLGNGNQAGEENRNVARMSSLLAGFPVGLPGATVNRLCGSGMEAVAIAARA
                     IAAGEGDAFLAGGVESMSRAPYVLAKASRGFARGPATLEDTALGWRFVNPELERMGHT
                     DSLGQTAENVARECGVGREEQDRFALRSHRNAVAAAERGYGEWLLPIELEEGELLSDD
                     GPRADTSLERLARLRPVFAADGSVTAGNSSPLSDGAAALLVVSAEFARARGLRPRARI
                     LGAASAGVAPRVMGLGPVPATAKLCARLGISVSDFDTIQLNEAFASQAIAVLREWGID
                     PEDDLVNPNGGAIALGHPIGCSGARLVGEVVRDLEAVGGKLGLATMCIGVGQGISVAL
                     ERVEG"
     gene            complement(4278..>4465)
                     /locus_tag="VFB52_04435"
     CDS             complement(4278..>4465)
                     /locus_tag="VFB52_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05611.1"
                     /translation="KFLRPVFIGDTVVVVTTVAEARETSKPDQGVVVQRVDVVNQDGG
                     TVQSGELVSLMTRRAAA"
BASE COUNT          647 a         1620 c         1522 g          676 t
ORIGIN      
        1 agccgcgcgc cggcaacgat ctctggggag ggcttccagc acaggggcgg cgcgacggcg
       61 ctctcgattt ccgctacccg gagcatgccg gcgatggcgg gcacggcggc gtcgaccatg
      121 gtcaggcccc ctgcccgtcg aggacgagcg tcctcaggct ctccagactg cgccggtaag
      181 tgtcttctac gcccgcggcc agccgctcct cgtcggcagc ctcgacggca gtgagctcgc
      241 cgcgccagag gacggtcgca gagtctccct caccactcac ctcgaagcgt ccgcggaagg
      301 cgcgcattga ggcgccctcc tccgccagct cgtactcgaa ccggcgctcc gcgtcggagt
      361 ggaagacgag ccgctcgacc accgtgccgc gcccgcggtc gagctcggcg tagcgcagat
      421 ctccctccat ccgcgctgcg tcgatcgagc tgatccagtt ggcagcgccg aaatcgccgg
      481 caatcgccca aacggcagcg ggtggagccg cgatcgagat ccgctgctcg accggcctca
      541 tccgtgcctg ccccgctcct tcggaatcac ctcgagccgg ccaaagctgc cgcggtagta
      601 ggccagcgga ctgccctcgc ccgcctccgc cacctcgacc aggccgatga aaatgatgtg
      661 ggtggcgctg gtcacccgct cggtgacctg gcactcgatg tgggcgagcg cgtcctcgag
      721 cagcggtcgc tcgtgagagc gtagcttcgg cggctcctcg tgaccgaacg gcgcgacgtc
      781 gaacttgccg gcgtccttgg tcgcgaagtg cccggccagg tgctcctggt cctcggtgag
      841 aatgttgacg acgaagtagc ctgattgatc gatcgccttg ccggtctccg agtcgcgatt
      901 gaggcagacc agcaacatcg gcggctcagc cgagaccgag gtgacggcgt ttgccgtggt
      961 tccgaagctg cgctcgccaa ctgcggtggt gaccacggtg acgccgctgg caaagtgacc
     1021 gatcacttcg cggtactgga cgctggtcgg cggacgccgc gtcattcgct cctccatggc
     1081 aagcatctgc tgctcctcct ccgttgccgg gctcccaacc taccggtcat ttggttgata
     1141 gtagcagccg cggaacggca ccgcccctgc ctgcggcggt ccactctgaa gggcgttaag
     1201 cggctttggc tttggctttg cgcttcttgg cgcggggccg tttgtgacct ttggacccgg
     1261 tcacggtggc gacgatcatg tcggcgtaga cctcggagat gtgctcagcg gagtcgggtc
     1321 cgccgcggcg gtaccagatc ggcagcccat tgatcgcgga gagaatgaag aagcaggcgt
     1381 agtgggtgtc gagatcggga tcggccccgc cgtccgcctt gcaggcgcgg atcatgcgct
     1441 ccacccgcgc ctcgtactcg tgccgcgtct gcgtgacggc ggccaggcgc tcgccggtga
     1501 ggtgcttcca ctcgttgaag tagaggctcg cccgctcgac gttttccagg taccagagcg
     1561 accacaatcg cgcgaactcc cgcaggcggt cgaccgcggg aacgtcgagt gcctccatct
     1621 cctccatcag cgcaaaggac tggcggtcgg actcttcgaa gatcctggcc agcaggtctt
     1681 ccttggagtc gatgtagtgg tagaggcttc ccttcaacac gccaacgcgg tcagcgacgt
     1741 cttggatcga agtggccgag taccccttgt cgtggaaggt ctcgacggcg gcctgaatca
     1801 catcgtcggc gcgattgcgc cgttccccgt cctctctgct aaccccccct gccttgctcg
     1861 ccaactttct cctccgcctt taactagagg gcgatccctc tgctggacgt tgccctttcg
     1921 aaacgctacg cgggaaatac ttaggaataa tcctagctag ttgaccaccc gaacgtttgg
     1981 aaggttcatc gaaccttaag cagccagact gcgcccacca caggcgagca cgacctgacc
     2041 ggttacgtag ccggccgcgg gcgacgcgag aaaggcgact agctcggcga cgtcgtcggg
     2101 gctgccaatc ctgcccgccg ggatccgccc taccagcttc tccagcacct cggccggcat
     2161 cgccgcggtc atcggcgtgt cgatcagccc cggagcgaca gcgttgaccc gcacccgggg
     2221 cgcccactgc agggccagcg cccgggtggc gccgaccagg cctgacttcg agctgacgta
     2281 gttggcctgg ccgaagttgc ccagcgaggc acgcgagctc atgttgacga ccgccgcgcc
     2341 ctccgcgtac agcggctcca aggccatcgc gaggcggatc gcggcgacca ggttgacggc
     2401 gatcacggcc gcgaagtcat tctctgaaag cttcgtcgcg cggccgtcgc gggtgatccc
     2461 ggcgttgttg acgaggagcg cgaccgctcc ggactcagcg gcggtggtgg cgaccgcggc
     2521 ggcgccctcg gcggcggtca agtcggcgac cacggcacgg gcgctcgccc catcccgctc
     2581 cagctccgcc gccctcgcat cgaccgcggg gtcgaggtcg accaggagaa gctcgtgccc
     2641 ggccgcggcg agccgcgcgg cgatccccgc cccgagtccg ccggcggtgc cggcgccggt
     2701 gaccacggcg agcccgctca cggtgagact ttcccgaggt gggcggctcc gacccgaatc
     2761 agctggccgc tgagcgcgga gccggccagc cattcggccg cgccgcgcgc cgcctcctcc
     2821 tcgtcgcggt gggcgaccgc gttgacccgc cagcccggtt tcgcgccctc gagggcgagc
     2881 gcccggacaa ggccgagcaa gccggtggcg agggcggcgt cggcaacctc tctctggccg
     2941 agcaggtcgc ggtcgtcgag caggacgacg accgggcgct cggcgatgac cgcgtcgcgg
     3001 gcggtggcga aggccgcggt cagcagcggg gtgattgcgt cgaggtccgc ggcggggggc
     3061 tgagcgagga tcagcggctc cgccatctca gccctcaacc cgctccagcg cgactgagat
     3121 cccctggccg acgccgatgc acatcgtcgc caggccgagc ttgccgccaa ccgcctcgag
     3181 gtcgcgcacg acctcgccga ccagccgcgc ccccgaacat ccgatcgggt gaccgagcgc
     3241 gatcgcgccg ccgttcgggt tgaccagatc gtcctcggga tcgatccccc actcgcgcag
     3301 gacggcgatc gcctgcgagg cgaaggcctc gttgagctgg atcgtgtcga agtccgaaac
     3361 cgagatcccg agccgggcgc agagcttcgc ggtcgccggg accggaccca gccccatcac
     3421 ccgcggcgcc accccggcac tggcggcgcc gaggatgcgg gcccgcgggc gcaagccgcg
     3481 cgcgcgcgca aactccgcgg agacgaccag cagcgccgcg gcgccgtcgc tgagcgggct
     3541 cgagttgccg gcggtgaccg agccgtcggc ggcgaagacg ggacgcaggc gggcgaggcg
     3601 ctccagcgag gtgtcggccc gcggcccgtc gtcgctcagc agctcgccct cctccagttc
     3661 gatcggcagc agccactcgc cgtatccccg ctcggcggcg gcgaccgcgt tgcggtggct
     3721 gcgcagcgcg aagcggtcct gctcctcgcg gccgacgccg cactcgcggg cgacgttctc
     3781 tgcggtctgg ccaagcgagt cggtgtggcc catccgctcc agctcggggt tgacaaagcg
     3841 ccagccgagc gccgtgtcct cgagcgtcgc cggtccgcgc gcgaagccgc ggctcgcctt
     3901 cgccagcacg tagggggcgc ggctcatcga ctcaacgccg ccggcgagga aggcgtcgcc
     3961 ctcgccggcg gcgatcgccc gggcggcgat cgcgaccgcc tccatgccgc tgccgcagag
     4021 ccggttgacg gtcgcccccg gcaggcccac cgggaagccg gcgagcagcg aggacatccg
     4081 cgccacgttg cggttttcct cgccggcctg gttgccgttg ccgaggtaga cgtcgtcgag
     4141 ctcggccggg tcgaccccgt tgcgctcgac cagcgccgcc agtgcctgcg ccgcgaggtc
     4201 gtcgggccgg acgccggcca gggagccgcc gtagcgcccc atcggggtgc gcagcgcgtc
     4261 gaccaccact gctgcgctca cgccgccgcc cgccgggtca tcagcgagac cagctctccc
     4321 gactgcacgg tcccgccgtc ctggttgacg acgtcgaccc gctgcacgac cacgccctgg
     4381 tcgggcttcg aggtctcgcg tgcctcggcc acggtcgtga ccacgactac ggtgtcgccg
     4441 atgaagaccg ggcgcaggaa cttcc
//
LOCUS       DASRIG010000161         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2697684, whole genome shotgun sequence.
ACCESSION   DASRIG010000161 DASRIG010000000
VERSION     DASRIG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697684"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..808)
                     /locus_tag="VFB52_04440"
     CDS             complement(56..808)
                     /locus_tag="VFB52_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05612.1"
                     /translation="MNRRAICLGVVAILVATTVTAGCGGSSDPTVSRTKVPSPDTHQF
                     VELPTRRCSTQLAYEGVSPVPLDARTPTALPASAPHSDLAAYTNTLGTTLIGPKGWEC
                     RSGAGVDGSEFMAIAPSLPEDEFDPSKTPAEAITLRSIPACLGCQAEALCSLFPDAPP
                     VTAYSPRLPCDEKPLRERAEILGNYFATFEDPPRVAGLGEPSGGANPAIGVIAYSHPS
                     GVGKVTCTVSARRRPVCSQIVLAAITSLVTRS"
     gene            complement(805..1791)
                     /locus_tag="VFB52_04445"
     CDS             complement(805..1791)
                     /locus_tag="VFB52_04445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO05613.1"
                     /translation="MSATLVTGATGTVGHAIATQLVATGAEVRALVRDLERAKTLLPA
                     GVEPVAGDVTNPASVAAAVAGCERVFHAAGLPEQWRLDTADFRRVNIEGTANVVDACL
                     AHGVERLVYTSTIDVFAWTPGKPFDESQLDPQPRPTAYERSKQEADRLVVAALERGLD
                     AVFLHPSAVYGPAPVVTVALNDFLIRLAKRKIPMLLPGGMPVVHAEDVAAGHLAAADQ
                     ASSGERFILSGPYLTLREIALAVREQIPRAKVPPVMPMAFARAIAGVGESVAKLTKHP
                     PLIARGEQIFLASHPVPVADRAGNTLGWQSRPVDQGFAETLASFRERGLLAS"
     gene            complement(1827..3068)
                     /locus_tag="VFB52_04450"
     CDS             complement(1827..3068)
                     /locus_tag="VFB52_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZO05614.1"
                     /translation="MSDLSAQQARRLALAAQGFGRPRPNGRVDARHLRRAIDDVGLLQ
                     LDSVNVFCRSHYMPVFSRLGPYPREALDRLAWHENPGGKARRAGQRDLIEYWGHEASL
                     LPVELQPLLRWRMARADKLAWKNVARVAAEQPELVEFVRGMVRERGPIRASELAAKGR
                     PREPGEMWSWSEEKTALEYLFFAGQVCAARRVNFERLYDLPERVLPPAVLDAPTPTQE
                     EAQRRLILIAAKRLGVATEADLGDYFRLPRTESKARVAELAEGGALTSVSVEGWRQPA
                     YLAAERPTGLRRAAAARALLTPFDSLVWARERTERIFGFRYRIEIYVPAPKRVYGYYV
                     LPFLLGDRLVARVDLKSDRQAGVLRVQSAFAEEHADTALVANELADELRQLSEWLGLG
                     GVSVARKGDLAAPLCRALSRP"
     gene            complement(3061..>3667)
                     /locus_tag="VFB52_04455"
     CDS             complement(3061..>3667)
                     /locus_tag="VFB52_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05615.1"
                     /translation="MYDLRIHVIRDDSAIEVSGLALLPIGLAIVAITAACFFLNAVFG
                     FAVAQEGTPRVRPAVEEARRHLPAILAWGGAFGAALALCMTVLARSEDFWFAVALGTV
                     AGLTMVAYVAVPARLAGVRSGQSLRDRLSAGAVGAALGFMVSAPPYLLGRIGLLMIGS
                     PILRIPGVIALAVAVALQAGATGAVRAVKMGAKLGGAPKSE"
BASE COUNT          521 a         1210 c         1444 g          492 t
ORIGIN      
        1 cccggcgaga tacttctaga ttcataacta ttatgaactt agcaggacga tctcctcagg
       61 atcgggtgac caaggaggtg atcgcggcga ggacgatctg cgagcagacc ggtcggcggc
      121 gagcggagac cgtgcaggtg accttgccga cgccgctcgg gtgcgagtag gcgatcacgc
      181 cgatcgccgg gttggcgccg ccgctcggct cgcccaggcc cgccactcgc ggcggatctt
      241 cgaaggtcgc gaagtagttg ccgagaatct cggcccgctc gcgcagcggc ttctcgtcgc
      301 agggcaggcg aggcgagtag gccgtcaccg gcggcgcgtc ggggaagagc gagcagagtg
      361 cttccgcctg gcatcccagg caagccggga tgctgcgcaa ggtgatcgct tccgcgggcg
      421 ttttcgacgg gtcgaattcg tcttccggca ggctcggggc gattgccatg aattcgctcc
      481 cgtcgactcc ggcgcccgag cgacattccc accccttcgg cccgatcagg gtggtgccaa
      541 gcgtgttggt gtaggcggcg aggtcggaat gcggtgcgct ggctggcagg gcggtcggcg
      601 tccgggcgtc gagcgggacc ggggagacgc cttcgtaggc gagctgggtg gagcagcggc
      661 gcgtgggaag ttcgacgaac tggtgggtgt cgggggaggg aaccttggtg cggctgaccg
      721 tcggatccga gctgccgccg cagccggccg tcaccgtggt cgcgaccagg atcgcgacca
      781 cgcccagaca gattgcgcgc cggttcatga ggcgagtagg ccgcgctcgc ggaaggaagc
      841 cagcgtttcg gcgaacccct ggtcgacggg gcggctctgc caccccaggg tgttgccggc
      901 gcgatcggcg accggaacgg ggtgtgaggc aaggaagatc tgctcgccgc gggcgatcag
      961 gggcgggtgc ttggtcagct tggcgaccga ctccccgacg ccggcgatcg ctcgcgcgaa
     1021 ggccatcggc atcaccggcg gcactttggc gcgggggatc tgctcgcgga cggcgagggc
     1081 gatctcgcgc agggtcaggt aggggccgga gaggatgaag cgctcgccgg agctggcctg
     1141 gtcggcggcg gcgaggtggc cggcggcgac gtcctcggcg tggaccacgg gcatgccgcc
     1201 cggcagcagc atcgggatct tgcgtttggc gaggcgaatc aggaagtcgt tgagggcgac
     1261 ggtcacgacc ggcgcggggc cgtagacggc ggaggggtgg aggaagaccg cgtcgaggcc
     1321 gcgctcgagg gcggcgacga cgaggcggtc ggcctcctgc ttggagcgct cgtaggcggt
     1381 ggggcgcggc tgcggatcga gctgggactc gtcaaagggc ttgcccggcg tccaggcgaa
     1441 gacgtcgatc gtgctggtgt agacgagacg ctcgaccccg tgggcgagac aggcgtcgac
     1501 cacgttggcg gtgccctcga tgttgacgcg ccggaagtcc gctgtgtcca ggcgccactg
     1561 ctcggggagc ccggcggcgt ggaagacgcg ctcgcagccg gcgacggccg cggccacgct
     1621 ggcggggttg gtgacgtcgc cggcgaccgg ctccactccg gcggggagca gggtcttggc
     1681 gcgctcgagg tcacgaacca gggcccggac ctcggctccg gtcgcaacca gctgggtggc
     1741 gatcgcgtgg ccgacggtgc cggtcgctcc ggtgacgaga gtcgcgctca tctggggaat
     1801 ctatggccga acgacgcctg agcgcctcag ggtcggctga gtgcgcggca cagcggcgcg
     1861 gcgaggtcgc ctttgcgggc taccgagacg ccgccgaggc cgagccactc gctgagctga
     1921 cgcagttcgt cggccagctc gttggcgacg agggccgtgt cggcgtgctc ctcggcgaag
     1981 gcgctctgca cgcgcaggac gccggcctgc cggtcgctct tcaggtccac ccgggcgacc
     2041 aggcggtcgc cgagcaggaa ggggaggacg tagtagccgt agacccgctt cggcgccggc
     2101 acgtagatct cgatccggta gcggaagccg aagatccgct cggtccgctc gcgcgcccag
     2161 acgagtgagt cgaacggggt gagcagcgcc cgcgccgccg cggcgcggcg caggcccgtg
     2221 ggccgctcgg ccgcgaggta ggccggctgg cgccagccct cgacgctgac ggaggtgagg
     2281 gcgccgccct cggctagctc ggcgacgcgg gccttcgact cggtgcgggg caggcggaag
     2341 tagtcgccga ggtcggcctc ggtcgccacc cccagccgct tggccgcgat caggatcagc
     2401 cggcgctgcg cctcctcctg cgtgggggtc ggcgcgtcga ggaccgcggg tggcaggacc
     2461 cgctcgggca ggtcgtagag ccgctcgaag ttgacccgcc gcgccgcgca cacctgcccg
     2521 gcgaagaaca ggtactcgag cgcggtcttc tcttcgctcc agctccacat ctcgcccggc
     2581 tcccgagggc gccctttcgc cgccagctcc gaggcccgga tcggcccgcg ctcgcgcacc
     2641 atcccgcgca cgaactcgac cagctcgggc tgctcggccg ccacccgcgc cacgtttttc
     2701 caggccagct tgtcggcccg ggccatccgc cagcgcagca ggggctgcag ctcgaccggc
     2761 agcagcgagg cttcgtggcc ccagtactcg atcaggtcgc gctgccccgc ccggcgggct
     2821 tttccgcccg ggttctcgtg ccaggccagc cggtcgaggg cctcgcgcgg gtaggggccg
     2881 aggcgggaga agaccggcat gtagtgggag cggcagaaga cgttgaccga gtcgagctgg
     2941 aggaggccga cgtcgtcgat cgcgcgccgc aggtgccggg cgtccacccg cccgttcggc
     3001 cgcggccggc cgaagccctg cgcggcgagc gccagccgcc gcgcctgctg cgcgctgagg
     3061 tcactcactt ttcggcgctc ctcccaattt cgcacccatc ttcaccgctc gcacggcgcc
     3121 ggtcgcgccg gcctgcaggg cgacggcgac ggcgagggcg atgacgccgg ggatgcggag
     3181 gatcggggag ccgatcatca gcaggccgat ccggccgagc aggtagggcg gggcgctgac
     3241 catgaagccg agggcggcgc cgacggcgcc ggcggagagg cggtcgcgca gggactggcc
     3301 ggagcggacg ccggcgaggc gggccggcac ggcgacgtag gcgaccatcg tcaggccggc
     3361 gacggtgccg agcgcgacgg cgaaccagaa gtcctcgcta cgcgcgagca cggtcatgca
     3421 gagcgcgagc gcggcgccga aggcgccgcc ccaggcgagg atcgcgggca ggtggcggcg
     3481 cgcttcctcg accgccgggc gcacgcgtgg cgtcccctcc tgggcgacgg cgaagccgaa
     3541 gacggcgttg aggaagaagc aggcggcggt gatcgcgacg atcgcgaggc cgatcgggag
     3601 cagggcgagg ccgctgacct cgatcgccga gtcgtcgcgg atcacgtgga ttcgcaggtc
     3661 gtacatc
//
LOCUS       DASRIG010000162         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1384788, whole genome shotgun sequence.
ACCESSION   DASRIG010000162 DASRIG010000000
VERSION     DASRIG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384788"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFB52_04460"
     CDS             <1..488
                     /locus_tag="VFB52_04460"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZO05616.1"
                     /translation="CFPEVHPEAPDLETDLTYLKTKVDSGAGFLITQLFFDNQVYFDF
                     VRAARERGIEVPIIAGVIPVASFAQTKRICELCDATIPGPLEAAFEAAGGDPDREFEL
                     GIAYASQQCAELLIAGAPGIHFYALNRAPATRAVLGALRASRPWERVRGDVGTLTGQA
                     E"
     gene            504..1301
                     /locus_tag="VFB52_04465"
     CDS             504..1301
                     /locus_tag="VFB52_04465"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HZO05617.1"
                     /translation="MGKGDREREREQLRQERLEAEASEKSSGRNRLIIGYAIASTAVI
                     AIAVAIFVIASGGGDDSAGGDAHINLNAAVGSTNGVQPDDRAGIVPVAAKTTNLQAAA
                     KAAGCKLELNLPDEGSSHLELGEPAPEYKTNPPTSGNHIVPPYQQADGAYSEMPEEIN
                     LVHTLEHGRLEIQYAPDLPEKAQLELKGLFDTMYGATLLFPNDKMDYEVAATTWTNLL
                     GCPTYEGQATLDAIRAFGRQTWGKFGGEPVEGFTFSGPTPAEPEEPN"
     gene            complement(1321..2253)
                     /locus_tag="VFB52_04470"
     CDS             complement(1321..2253)
                     /locus_tag="VFB52_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO05618.1"
                     /translation="MTARAELLEDGGAAAVSEDFFRSREFLAAEGATHTLRIETASGE
                     ALLAPLLVREIEGSDARDAISPYGYPGLVPSPAVPLDPAAIDFSATGLVSVFLRHTLD
                     PPSLAGTTKRNVVQIADPALPPKSRPSDRRQVRRNLEAGYELRLVPGAETSAAERAGF
                     LDLYEQTMRRTGASTHYFFSAAYFDRILRAERTWLALATAPGAELAAASIAAVSDGYL
                     HYYLSGSADSRLREAPMKNVVTRLVEHAAELGLPLNLGGGLARGDALEEFKRGFANRE
                     LAWHSSELICDASAYAELSAGREAGGFFPAYRAP"
     gene            complement(2250..2798)
                     /locus_tag="VFB52_04475"
     CDS             complement(2250..2798)
                     /locus_tag="VFB52_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO05619.1"
                     /translation="MSDRPAVSLRRFEPGDAPAVHRWFNNEEATKTLMEQRSAFSLEE
                     AEGWVGRAMNEDGEDRKFAIVVEGYEEPVGFTALYGLFRQTAPELGALIGDDVRGKGV
                     GRRAEALTIAKAFDEYDAHRVYGRIPARNEGAKRAVASLGWVHEGTMRGHLRREGLPA
                     DDCEVWGVLPDEFRAAAADLLE"
     gene            complement(2860..3423)
                     /locus_tag="VFB52_04480"
     CDS             complement(2860..3423)
                     /locus_tag="VFB52_04480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO05620.1"
                     /translation="MPRAFDILIAALALVVLSPFLLAAAVAIKLGSRGPVFYRQRRVG
                     LGDREFEMLKLRTMVQGSDPVGVGTVVTRDDPRVTRAGRFLRRTSLDEVPNLVNVLRG
                     EMAIVGPRPTIPAQVDDYTPFQRRRHEVLPGITGWAQVQGRAGIPWEERIELDVWYVD
                     HRSLALDLRILARSVWLVITAQGLAPD"
     gene            complement(3423..>3683)
                     /locus_tag="VFB52_04485"
     CDS             complement(3423..>3683)
                     /locus_tag="VFB52_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05621.1"
                     /translation="YLSPFLHVLVFLANLVLLGDGWFYYLTFILQLGLLGGALLAPYV
                     PSTPLRFARYYVLTTASIAAGLWDRWRRKRPTGRWEKAEGTR"
BASE COUNT          598 a         1246 c         1354 g          485 t
ORIGIN      
        1 cctgcttccc ggaggtgcac ccggaggcgc ccgacctcga gaccgacctc acctacctga
       61 agaccaaggt cgactccggc gccggcttcc tgatcaccca gctcttcttc gacaaccagg
      121 tctacttcga cttcgtacga gcggcgcgcg aacgcgggat cgaggttccg atcatcgccg
      181 gcgtgatccc ggtcgccagc ttcgcccaga cgaagcggat ctgcgagctt tgcgacgcga
      241 cgatcccggg cccgctggaa gcggccttcg aggcggccgg gggagacccc gaccgcgagt
      301 tcgagctcgg catcgcctac gcgtcccagc agtgcgcgga gctcctgatc gccggcgcac
      361 cggggatcca cttctacgcg ctcaaccgcg ctccggcgac ccgcgcggtg cttggagcgc
      421 tgcgcgcctc gcggccctgg gagcgggtgc gcggcgacgt cgggaccctg accggccagg
      481 ccgaatagtc ttcccgcctc agcatgggta agggcgacag agaacgcgaa cgcgagcaac
      541 tccgtcagga gcggctcgaa gcggaagcgt cggagaagag ctccggccgc aaccggctga
      601 tcatcggcta cgcgatcgcc tcgaccgcgg tgatcgcgat cgccgtcgcg atcttcgtga
      661 tcgccagcgg cgggggcgac gacagcgcgg ggggcgacgc ccacatcaac ctcaacgccg
      721 cagtcggctc gaccaacggc gtgcagccgg acgaccgggc cgggatcgtg cccgtcgcgg
      781 cgaaaacgac caacctgcag gcggcggcga aagcggccgg ctgcaaactc gaactgaact
      841 tgcccgacga gggcagctca catctcgaac tcggcgaacc ggcgccggaa tacaaaacca
      901 acccgccgac ctcgggcaat cacatcgtgc cgccctatca gcaggcggac ggcgcctact
      961 ccgaaatgcc ggaagagatc aaccttgtgc acacgctcga gcacggccgg ctggagatcc
     1021 agtacgcccc cgacctgccc gaaaaagcgc agctcgaact gaagggcctg ttcgacacga
     1081 tgtacggggc gacgctgctc ttcccgaacg acaaaatgga ttacgaggtc gcggcgacca
     1141 cctggaccaa cctgctcggc tgcccgactt acgagggaca ggcgacgctc gacgcgatcc
     1201 gcgccttcgg caggcagacc tggggcaagt tcggcggcga gccggtcgaa gggttcacct
     1261 ttagcggccc gaccccggcc gaacccgaag aaccgaacta gccgtagcgg cgggcccgag
     1321 ctagggcgcc cggtaggcgg ggaagaaacc gccggcctcg cggccggcgc tgagctctgc
     1381 gtaggcgctc gcgtcgcaga tcagctccga gctgtgccag gcgagctcgc ggttggcgaa
     1441 gccgcgcttg aactcctcca gcgcgtcccc gcgggcgagg ccgccgccga ggttgagcgg
     1501 cagaccgagt tcggcggcgt gctcgaccag gcgggtgacg acgttcttca tcggcgcctc
     1561 gcgcaggcgg gaatcggcgc tgccggagag gtagtagtgg aggtagccgt cgctgaccgc
     1621 ggcgatcgag gcggcggcca actcggcgcc gggcgcggtc gcgagtgcca gccaggtgcg
     1681 ctcggccctg aggatgcggt cgaagtaggc ggcgctgaag aagtagtggg tggaggcacc
     1741 ggttcgccgc atcgtctgct cgtagaggtc gaggaagccg gcgcgctcgg cagcgctcgt
     1801 ctccgctccg ggaacgagcc gcagctcgta gcccgcctcg aggttgcggc ggacctggcg
     1861 gcggtcgctg gggcggctct tcggcggcag cgccgggtcg gcgatctgga ccacgttgcg
     1921 cttcgtggtc ccggcgaggg acggcgggtc gagcgtgtgg cggaggaaga cgctgaccag
     1981 cccggtggcg gagaagtcga tcgcggccgg gtcgagcggc accgccgggc tggggacgag
     2041 gccggggtaa ccgtagggcg agatcgcgtc gcgggcgtcg gagccctcga tctcgcggac
     2101 cagcagcggg gcgagcagag cctcgcccga ggcggtctcg attcgaaggg tgtgcgtggc
     2161 gccctcggcg gcgagaaact cgcgcgagcg gaagaagtcc tcagagacgg cggccgcgcc
     2221 gccgtcttcc agcagctcgg cccttgcggt cactccaaca ggtccgcggc ggcggcgcgg
     2281 aactcgtccg ggagcacacc ccagacctcg cagtcgtcgg ccggcaggcc ctcgcgccgc
     2341 aggtgcccgc gcatcgtccc ctcgtgcacc cagccgaggc tggcgacggc gcgcttggcg
     2401 ccctcgttgc gggcggggat gcgaccgtag acgcggtggg cgtcgtactc gtcgaaggcc
     2461 ttggcgatcg tcagtgcctc ggcgcggcgg ccgacgccct tgccgcgcac gtcgtcgccg
     2521 atcagcgccc cgagctccgg cgcggtctgg cggaagagac cgtagagcgc ggtgaacccg
     2581 accggctcct cgtagccctc gaccacgatc gcgaacttgc ggtcctcgcc gtcctcgttc
     2641 atcgcccggc cgacccagcc ttcggcttcc tccaacgaga aggcggaccg ctgctccatc
     2701 agcgtcttcg ttgcctcctc gttgttgaac caacggtgca cggcaggcgc gtcaccaggc
     2761 tcgaagcggc gcaaactgac tgcgggacgg tcgctcacag cggcagccta gccacccctc
     2821 tgcatttgtc gcctcctggg cgacaaatgc agggtgtcgt tagtcggggg cgaggccttg
     2881 ggcggtgatg acgagccaga ccgagcgggc gaggatgcgc aggtcgaggg cgaggctgcg
     2941 gtggtcgaca taccagacgt cgagctcgat ccgctcctcc caggggatgc cggcgcggcc
     3001 ctggacctgg gcccagccgg tgattccggg caggacctcg tggcggcggc gctggaacgg
     3061 ggtgtagtcg tcgacctggg cggggatcgt cggacgcggg ccgacgatcg ccatctcgcc
     3121 gcgcagcacg ttgaccaggt tcggcacctc gtcgagcgag gtgcggcgca gaaagcggcc
     3181 ggcgcgggtg acgcggggat cgtcgcgggt gaccacggtg ccgaccccga ccgggtcgga
     3241 gccctggacc atcgtgcgca gcttcagcat ctcgaactcg cggtcgccga ggccgacgcg
     3301 gcgctggcgg tagaagaccg ggccgcggct gccgagcttg atcgcgaccg ccgcggccag
     3361 caggaagggc gagagcacga ccagcgccag ggcggcgatc aggatgtcga aggcgcgcgg
     3421 catcagcgcg tgccctcggc cttctcccag cggccggtcg gccgcttgcg gcgccagcgg
     3481 tcccagagcc cggcggcgat cgaggccgtg gtcagcacgt agtagcgggc gaagcgcagc
     3541 ggcgtcgagg gcacgtaggg ggcgagcagg gcaccaccga gcaggccgag ctggaggatg
     3601 aaggtgaggt agtagaacca gccgtcgccc agcagcacga ggttggcaag gaagacgagc
     3661 acgtgcagga acgggctgag gta
//
LOCUS       DASRIG010000163         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3472516, whole genome shotgun sequence.
ACCESSION   DASRIG010000163 DASRIG010000000
VERSION     DASRIG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3472516"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFB52_04490"
     CDS             complement(<1..843)
                     /locus_tag="VFB52_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO05622.1"
                     /translation="MRILILGGDGYLGWPTALRFSARGHEVSVVDNFARRRWVEGAGS
                     DSLTPIADLETRIAVWEELSGDRIRSFLGTVEDGEFLDEVVAETKPDAVVHYGEQPSA
                     PYSMISRQTAVETQQANVVGNLNLLFSMRDHVPDAHLVKLGTMGEYGQPNIDIEEGYI
                     EIEHKGRKDTLPFPKLPGSLYHCSKVHDSTNIHFACRTWGLRATDLNQGVVYGVETEE
                     TARDERLITRFDYDELFGTVLNRFCLQAVIGHPLTVYGAGQQTRGFLNIRDTLNCVEL
                     AVTNP"
     gene            870..2243
                     /locus_tag="VFB52_04495"
     CDS             870..2243
                     /locus_tag="VFB52_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO05623.1"
                     /translation="MRERLENVCGGRFAAIAVISLTLLGLGLRLDYAMEAPQKPVDDA
                     RAYARISQALYEGEGYTQGRGYEHLQSASNYQPGLPLFVTGVYELRGEPDQTAARIVL
                     ALLAAFAVPFAFLLGRRLAGPAAGLIAAAAVAVYPALLEYTGMLMTEPVGTALLAGMT
                     LLYLRAREGTRRWPWAVAGVALGALVMLRPEYLLLVPILPLLALVAWDRSPRGSFPDS
                     VSDKEPRGGIDRALVGRAALMAGCALLVLLPWTVRNYVAFDRVVPISTGGGQVIYAGS
                     YLPAGPDPEAITEDVLAEHPWIREELGPAPGKIYRGQALAALAEREHPDESTDTALTR
                     MALKAYVDNLRNEPLDLAGFVAGKVWFGWTEPARGIMRFPLWRALQIAVLALGAVGLV
                     VGLLRRRFDVAAIAAILLVETLVQAIFIASPRRTLTLLPLTAALAGAGAVWLWDQRRK
                     VGRPSYQ"
     gene            complement(2207..2887)
                     /locus_tag="VFB52_04500"
     CDS             complement(2207..2887)
                     /locus_tag="VFB52_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05624.1"
                     /translation="MSRRLPLAFTLALVAALAFATAAGAATVSLKCAGKGAHNRDSAG
                     TILCAAEPGKARTISGTVRDDAGKPVATKVTVTFSDWIPSGGGGYTIKETSTKTISSQ
                     PSGAFSLPVKTSTRVSVEFAVGDEAAGVSGASAQADVARRLAVKLSKLGGGKVKLTVK
                     GAAGKKLKLYILDTSGYEIPGVPPKVTKTGSAVFDLGSRRGQFSYYVDAGIYTDLYWY
                     EGRPTFRL"
     gene            2989..4395
                     /locus_tag="VFB52_04505"
     CDS             2989..4395
                     /locus_tag="VFB52_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO05625.1"
                     /translation="MNAVADWIRGLPERHGRRTLIALAAIAFVGLVARAYVVVNPVDH
                     PADDSRAYFALAKSLYTEGSYGGPEFEDSSDWSPGAPLLYAAAFYATGGAREGTARIV
                     EALMGVAAIFVVYALGRRLGRRICRRESPEGDDGPSAAGSAVGLFAAAAVAVYPPFIH
                     STGELMSEPPAILTLPAAVLAFLWASERERPWYAWLAPGALFGLTAMFRPEYLFVGVV
                     FAIAAAFRPTGTGWERRAATALVMLGALVVVILPWSIRNQVVLDRTVPISTGGGKALY
                     VGTYLPADGEYQRVKAILVKEFEGRELDPHSRELEEVDPTPLFDEVAERYPDLPRDSA
                     LGKIGKEQLSDYLSEDPLEYLAMTVRKVGRMWSSGVGEAMSSTLGRVIQIALVVLGLA
                     GLTLLGLRRRWWELVALATPILLVTAVGAVSLAAPRRNEVLMTLVFPLAGLALFWVVS
                     ALSSGRSWRSPQASSPPN"
     gene            4359..5426
                     /locus_tag="VFB52_04510"
     CDS             4359..5426
                     /locus_tag="VFB52_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO05626.1"
                     /translation="MALTAGIFPAELTALRGVGLAIAAILIVYAVARRKAMRNADVLL
                     LLAAGVALLLVCGTEIVNGLLSAFSFEKGNNGRILGLAVFAIFFLFLLTLRALSVAAR
                     NSRQLSAALEGLAWEEFRQAGLPERFRGKIAILIPAYNEAENVGTVLGQIPREVCGVA
                     TEVLVVDDGSRDGTGDVAAEHGACVARHVTNRGGGAALRTGYRLMVESGAEIVVTLDA
                     DGQHRPDQMERLVKPILDGEVDVAHGSRVLGAADRNHFARELGIVFFNRVVSFITRTH
                     VTDCSNGYRAVRTTVLPQLVLRQEQFHTSEFMIEAIKRGIPAKEIPITVEARLHGHSK
                     KPAVFRYGVGFANAIVRTWLR"
     gene            5501..>5688
                     /locus_tag="VFB52_04515"
     CDS             5501..>5688
                     /locus_tag="VFB52_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05627.1"
                     /translation="MLDEEVEAELPGVELVEQQGAAVAAWSPDGRRIAIPVPGAIGVP
                     AGIGLRRPGGALLSRFEA"
BASE COUNT          732 a         2016 c         2023 g          917 t
ORIGIN      
        1 cgggttggtg accgccagct cgacgcagtt gagcgtgtcg cggatgttga ggaagccgcg
       61 ggtctgctgg ccggcgccgt agaccgtcag agggtggccg atcacggcct gcaggcagaa
      121 gcggttgagc acggtgccga acagctcgtc gtagtcgaag cgggtgatca ggcgctcgtc
      181 gcgggcggtc tcctcggtct cgaccccgta gacgacgccc tggttgaggt cggtcgcgcg
      241 caggccccag gtgcgacagg cgaagtggat gttggtcgag tcgtggacct tcgagcagtg
      301 gtagagcgag ccgggcagct tcgggaacgg cagggtgtcc ttgcggccct tgtgctcgat
      361 ctcgatgtag ccctcctcga tgtcgatatt gggctggccg tactcgccca tcgtcccgag
      421 cttcaccagg tgcgcgtccg gcacgtggtc gcgcatgctg aacagcaggt tgaggttgcc
      481 gacgacgttg gcctgctggg tctcgaccgc ggtctggcgg gagatcatcg agtagggcgc
      541 cgagggctgc tcgccgtagt ggacgacggc gtcgggcttg gtctcggcga ccacctcgtc
      601 gaggaactcg ccgtcctcga ccgtgccgag gaagctgcgg atgcggtcgc cggagagctc
      661 ctcccagacg gcgatccggg tctccagatc ggcgatcggg gtgagcgagt cggagccggc
      721 gccctcgacc cagcggcggc gcgcaaagtt gtcgacgacg gagacttcgt ggccgcgagc
      781 ggagaaacga agggcggtgg gccagccgag gtagccgtcg ccaccgagga tgaggattcg
      841 cattgggcgg cagcgtagct tgtagcgcct tgagggagcg cctggaaaac gtctgcggcg
      901 gccgtttcgc ggcaatcgcc gtgatctctt tgaccctgct tggccttgga ttgcggctcg
      961 actacgcgat ggaggcgccg cagaagccgg tcgacgacgc ccgcgcctac gcccggatct
     1021 cgcaggcgct gtacgagggc gagggctaca cccagggccg cggctacgag cacctgcagt
     1081 cggcttcgaa ctaccagccg ggcctgccgc tgttcgtcac cggggtctac gagctgcggg
     1141 gagagcccga ccagacggcg gcgcggatcg tgctcgcgct gctcgccgcg tttgccgtcc
     1201 cgttcgcctt cctgctcggg cgcaggctgg ccggcccggc cgccgggctg attgccgccg
     1261 ccgcggtcgc ggtctaccca gcgctgctcg agtacaccgg gatgctgatg accgagcccg
     1321 tcggcacggc gctgctggcc gggatgacgc tgctctacct gcgagcccgc gaagggacgc
     1381 ggcgctggcc ctgggccgtg gccggcgtgg cgctcggcgc gctggtgatg ctgcgccccg
     1441 agtacctgct gctggtcccg atcctgcctc tgctggcgct ggtcgcctgg gaccgttcac
     1501 cccgcggttc ctttccggac tctgtgtccg ataaggaacc acgcggcggg atcgaccggg
     1561 cgttggtcgg gcgggcggcg ctgatggccg gctgcgcgct gctcgtcctg ctgccctgga
     1621 ccgtgcgcaa ctacgtcgcc ttcgaccggg tggtgccgat ctcgaccggc ggcggtcagg
     1681 tgatctacgc cggcagctac ctgcccgccg gtcccgaccc ggaagcgatc accgaagacg
     1741 tgctcgccga acacccgtgg atccgcgaag agctggggcc ggccccgggc aagatctacc
     1801 gcggtcaggc gctggcggcg ctggccgaac gcgaacaccc cgacgagagc accgacacgg
     1861 cgctgacccg gatggcgctc aaggcttacg tcgacaacct ccgcaacgaa ccgctcgacc
     1921 tcgccggctt cgtcgccggc aaggtctggt tcggctggac cgagccggcc cgcgggatca
     1981 tgcgcttccc gctctggcgg gcgctgcaga tcgcggtgct cgcgctcggc gcggtcgggc
     2041 tcgtcgtcgg cctgctgcgg cggcgcttcg acgtcgccgc gatcgccgcg atcctgctcg
     2101 tcgagaccct cgtgcaggcg atcttcatcg cctccccccg tcgcaccctg acgctgctgc
     2161 ccctcaccgc ggccctggcc ggcgccggcg ccgtctggct ctgggatcag aggcggaagg
     2221 tcgggcggcc ctcgtaccag tagaggtcgg tgtagatgcc ggcgtcgacg tagtagctga
     2281 actgcccgcg gcgcgatccg aggtcgaaga cggcgctgcc ggttttggtc accttcggcg
     2341 ggacgccggg gatctcgtag ccggaggtgt cgaggatgta gagcttcagc ttcttgccgg
     2401 cggcgccctt gaccgtgagc ttgaccttgc cgccgccgag cttgctcagc ttcaccgcga
     2461 ggcggcgggc gacgtccgcc tgggcgctgg ccccggagac gcccgcggct tcgtcgccga
     2521 ccgcgaactc gacgctgacc cgggtggagg tcttgaccgg cagcgagaac gcgccgctcg
     2581 gctgcgagga gatcgtcttc gtgctggtct ccttgatcgt gtagccaccg ccgccgctgg
     2641 ggatccagtc ggagaaggtg accgtgacct tggtcgcgac cggtttgccg gcgtcgtcgc
     2701 ggacggtgcc ggagatcgtc cgcgccttgc ccggttcggc ggcgcagagg atcgtgccgg
     2761 cgctgtccct gttgtgcgca cccttgccgg cgcacttgag cgagaccgtt gcggcccccg
     2821 cggcggtggc gaaggccagc gcggcgacga gcgccagggt gaaggcgagc ggcaggcggc
     2881 gggacatcgg gcggatccta tgcgggcagt ggtccgccat tggccgccgc cttccccacc
     2941 cgggtaggaa ctacccatcg ggcaccgccc ggatagcctg agccggcctt gaacgccgtc
     3001 gccgactgga tccgcgggtt gcccgagcgc catggacgcc ggacgctgat cgcgctcgcg
     3061 gcgatcgcct tcgtcgggtt ggtcgcccgc gcctacgtcg tggtcaaccc ggtcgaccac
     3121 ccggcggacg actcgcgcgc ctacttcgcc ctcgccaagt cgctctacac ggaaggcagc
     3181 tacggcgggc ccgaattcga ggactccagc gactggtcgc cgggggcgcc actgctttac
     3241 gcggcggcct tctacgcgac cggcggcgcc cgcgaaggga ccgcgcggat cgtcgaggcg
     3301 ctgatgggcg tcgccgcgat cttcgtcgtc tacgcgctgg ggaggcggct ggggcggcga
     3361 atttgccgcc gcgagagccc agagggcgac gacgggccct ccgccgccgg ctcggccgtg
     3421 gggctcttcg ccgccgccgc ggtcgccgtc tacccgccgt tcatccactc gaccggggag
     3481 ctgatgagcg agccgccggc catcctcacc ctgcccgcgg ccgtgctcgc cttcctctgg
     3541 gcgagcgagc gcgagcggcc ctggtacgcc tggctcgccc ccggtgcgct cttcggcctg
     3601 accgcgatgt tccggcccga atacctcttt gtcggcgtcg tcttcgcgat cgcggcggcg
     3661 ttccggccga ccgggaccgg ctgggaacgg cgggccgcga ccgcgctggt gatgctcggc
     3721 gcgctcgtcg tcgtcatcct gccctggtcg atccgcaacc aggtcgtgct cgaccgcacc
     3781 gtgccgatct cgaccggcgg cggcaaggcg ctctacgtcg gcacctacct gcccgccgac
     3841 ggcgaatacc agcgggtcaa ggcgatcctg gtcaaggagt tcgaaggccg cgaactggac
     3901 ccgcactcgc gcgaactgga ggaagtcgac ccgacgccgc tgttcgacga ggtcgccgaa
     3961 cgctaccccg acctgccgcg cgactcggcg ctcggcaaga tcggcaaaga acagctctcc
     4021 gactacctct ccgaagaccc gctcgaatac ctggcgatga cggtgcgcaa ggtcgggcgc
     4081 atgtggtcga gcggggtcgg tgaagcgatg agcagcacgc tcggccgggt gatccagatc
     4141 gcgctcgtcg tgctcgggct ggccggcctg accctgctcg ggctgcgccg gcgctggtgg
     4201 gagctggtcg cgctggccac cccgatcctg ctcgtcaccg cggtcggcgc ggtctcgctg
     4261 gccgccccgc gccgcaacga ggtgctgatg acgctggtct tcccacttgc cggcttggcg
     4321 ctgttctggg tggtctcggc cctatcctct ggccgctcat ggcgctcacc gcaggcatct
     4381 tccccgccga actgaccgcg ctgcgcgggg tcggcctggc gatcgccgcg atcctgatcg
     4441 tctacgcggt cgcccgccgc aaggcgatgc gcaacgccga cgtgctgctg ctgctcgccg
     4501 ccggggttgc cctgctgctc gtctgcggca ccgaaatcgt caacgggctg ctctccgcct
     4561 tctcgttcga gaaaggcaac aacggccgca tcctcggcct cgccgtgttc gcgatcttct
     4621 tcctcttcct gctcaccctg cgggcgctct cggtggcggc gcgcaactcg cgccagctct
     4681 ccgccgcgct cgagggcctg gcctgggagg agttccgcca agcggggctg ccggagcgct
     4741 tccgcggcaa gatcgcgatc ctgatcccgg cctacaacga ggccgaaaac gtcggcaccg
     4801 tgctcgggca gatcccgcgc gaggtctgcg gggtcgcgac cgaggtgcta gtcgtcgacg
     4861 acggctcccg cgacggcacc ggcgacgtcg ccgccgagca cggcgcctgc gtcgcccgcc
     4921 acgtcaccaa ccgcggcggc ggggccgccc tgcgcaccgg ctaccggctg atggtcgaat
     4981 ccggcgccga gatcgtcgtc accctcgacg ccgacggcca gcaccgcccc gaccagatgg
     5041 agcggctggt caaaccgatc ctcgacggcg aagtcgacgt cgcccacggc tcgcgcgtgc
     5101 tcggcgccgc cgatcgcaac cacttcgccc gcgagctcgg gatcgtcttc ttcaaccggg
     5161 tggtctcgtt catcacccgc acccacgtga ccgactgctc gaacggctac cgcgcggtgc
     5221 gcaccacggt gctgccgcag ctggtcctgc gccaggagca gttccacacc tccgagttca
     5281 tgatcgaggc gatcaagcgc gggatccccg ccaaggagat cccgatcacg gtcgaagcgc
     5341 gcctgcacgg ccactcgaag aagccggcgg tgttccggta cggcgtcggc ttcgccaacg
     5401 ctatcgtgcg cacttggctt cgctagctcg cgcccggccc cgcctcctct ttctgctccc
     5461 cgcactgctc tttttggcgc tgctggccag cggctgccgc atgctcgacg aagaggtcga
     5521 ggcggagctg cccggggtcg agctggtcga gcagcagggc gcggccgtcg ccgcctggtc
     5581 gcccgacggc cgccggatcg cgatcccggt cccgggagca atcggggtcc cggcggggat
     5641 cgggctgcgc cgccccggcg gggcgctgct gtcgcggttc gaggctca
//
LOCUS       DASRIG010000164         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_74251, whole genome shotgun sequence.
ACCESSION   DASRIG010000164 DASRIG010000000
VERSION     DASRIG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74251"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFB52_04520"
     CDS             complement(<1..702)
                     /locus_tag="VFB52_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO05628.1"
                     /translation="MTWPFEGLPTLWVVLFSVCLVVVVAMFLWTLLLFVRGQKAIVRP
                     PQPPPEGADAFSWVFLVPAMNEEVTIADSVQRLIELPVSRRRVIVIDDGSEDRTAEIL
                     AGIDHPDLFVLHRVKPNAQKGKAAALNYAYQSLRAHTDRENTIVVIVDADGRLRPSAP
                     HFAAGHFADPNVGGVQSLVRIYNRQNPLTWLQDVEFAVYGHLFQAGRDHWGTAGMGGN
                     GQFNRLAALDAVADEE"
     gene            complement(708..2123)
                     /gene="wecB"
                     /locus_tag="VFB52_04525"
     CDS             complement(708..2123)
                     /gene="wecB"
                     /locus_tag="VFB52_04525"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZO05629.1"
                     /translation="MFESATKPLPEHKLVVPIVVGTRPEAIKLVPIIVALRESDVYEP
                     VVVSTGQHSRLVEYIFELAEIKPDVTLWAGSRRANLNERVASVMQRFEDFCYERFESD
                     FEEAASADDVLSGRHPAAVLVHGDTSSAMAAALSAFHLRIPVMHVEAGLRTGGSNLTP
                     FPEELNRQVISTIAAMHFAPTSANLQNLVRENIPVGQVFVTGNTGIDALHWSSQLEDI
                     RFANPELQALVDGESRIVVITAHRRENWGDGLRGIAEGVARLARDQHDVDFVLPVHPN
                     PRVREVLTERLTGLENVLLTEPLGYATFSRLLGRCHMVITDSGGIQEEAPSLGKPVLV
                     TRETTERTEGLAAGTLRLVGTDPDLIHAEGTRLLDSESAYREMAEAENPYGDGHAAER
                     IVGALEHVLLGGEPPTQFGPGYSRATISVAAGFRPTPGLALEQLKQAFGDSEPAPAPE
                     IVVTEATSGGAEVGTSYLIES"
BASE COUNT          346 a          757 c          862 g          356 t
ORIGIN      
        1 ctcctcgtcg gcgacggcgt cgagggcggc gaggcggttg aactggccgt tgccacccat
       61 ccccgccgtc ccccagtgat cgcggcccgc ctggaagagg tggccgtaga cggcgaactc
      121 gacgtcctgc agccaggtca gcgggttctg gcggttgtag atccggacca gcgactggac
      181 gccgccgacg ttgggatcgg cgaagtggcc ggcggcgaag tgcggtgccg aggggcgcag
      241 gcggccgtcg gcgtcgacga tcacgacgat cgtgttctcg cggtcggtgt gggcgcgcag
      301 cgactggtag gcgtagttga gcgccgccgc cttgcccttc tgcgcgttcg gcttgacccg
      361 gtgcaggacg aagaggtcgg ggtggtcgat cccggcgaga atctcggcgg tgcggtcctc
      421 ggagccatcg tcgatcacga tcacgcgccg gcgcgagacc ggcagctcga tcagccgctg
      481 gaccgagtcg gcgatcgtca cctcctcgtt catcgccggc accaggaaga cccaggagaa
      541 ggcgtcggcc ccctcgggcg gcggctgcgg cgggcgcacg atcgccttct ggccgcgcac
      601 gaacagcagc agcgtccaga ggaacatcgc gacgacgacg accaagcaca cgctgaagag
      661 gacgacccag agggtcggca gcccctcgaa cggccaggtc accacggcta gctctcgatc
      721 aggtagctgg tccccacttc ggcgccgccg gacgtcgcct cggtgacgac gatctccgga
      781 gccggtgccg gctcggaatc gccgaacgcc tgcttcagct gctccagcgc caggccgggc
      841 gtcggccgga agccggcggc gaccgagatc gtcgcgcggc tgtagccggg gccgaactgg
      901 gtcggcggct cgccgccgag caggacgtgc tcgagcgcgc cgacgatccg ctcggcggcg
      961 tgaccgtcgc cgtagggatt ttccgcctcg gccatctcgc ggtaggccga ctcgctgtcg
     1021 agcaggcgcg tgccctcggc gtggatcagg tcggggtcgg tgccgaccag gcgcagggtg
     1081 ccggcggcga ggccctcggt gcgctcggtc gtctcgcggg tgaccagaac cggcttgccg
     1141 agcgagggcg cctcttcctg gatcccgccg gagtcggtga tcaccatgtg acagcggccg
     1201 agcagccggg agaaggtcgc gtagccgagc ggctcggtca gcagcacgtt ctcgagcccg
     1261 gtgaggcgct cggtcagcac ctcgcgcacg cgcgggttcg ggtggaccgg gaggacgaag
     1321 tcgacgtcgt gctggtcgcg ggcgaggcgg gcgacgccct cggcgatccc gcgcaggccg
     1381 tcgccccagt tctcgcgccg gtgcgcggtg atcacgacga ttctcgattc gccgtcgacc
     1441 agcgcctgca gctcggggtt ggcgaacctg atgtcctcga gctgcgagga ccagtggagg
     1501 gcgtcgatcc cggtgttgcc ggtgacgaag acctggccga ccgggatgtt ctcgcggacc
     1561 aggttctgca ggttggccga ggtcggggcg aagtgcatcg cggcgatcgt cgagatcacc
     1621 tggcggttca gctcctccgg gaacggggtg aggttgctgc cgccggtgcg cagaccggcc
     1681 tcgacgtgca tcaccgggat ccgcaggtgg aaggcggaaa gcgccgccgc catcgccgag
     1741 ctggtgtcgc cgtggacgag gaccgcggcc gggtggcggc cgctgagcac gtcgtcggcg
     1801 ctcgccgcct cctcgaagtc ggattcgaag cgctcgtaac agaagtcttc gaagcgctgc
     1861 atcaccgagg cgacccgctc gttcaggttg gcgcgccgcg agccggccca gagggtgacg
     1921 tcgggcttga tctcggcgag ctcgaagatg tactcgacca ggcggctgtg ctgaccggtg
     1981 gagacgacga ccggctcgta gacgtcgctc tcgcgcaggg cgacgatgat cggcaccagc
     2041 ttgatcgcct cggggcgcgt cccgaccacg atcgggacga caagcttgtg ctcgggcagg
     2101 ggtttggtag ccgactcgaa catgcgggtt cccggcaggt atcggtgcgt ggcggcacta
     2161 acttgagact ggcccgcctg cctaaccgaa gatttgttcg agcagatccc cggccggccc
     2221 gaggaagttc tggaaggttt cgaaggcgga accggcgtct tcgaccagtt cgatcgcctg
     2281 gtcgagtaca tcttcgagct cgccgagatc aagcccgacg t
//
LOCUS       DASRIG010000165         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_74370, whole genome shotgun sequence.
ACCESSION   DASRIG010000165 DASRIG010000000
VERSION     DASRIG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74370"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1294
                     /locus_tag="VFB52_04530"
     CDS             143..1294
                     /locus_tag="VFB52_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HZO05630.1"
                     /translation="MALPTQEQITERLRAVIDPELRRSIVELGMVRSILVQESGRVEV
                     VVSLTTAGCPIRSHFEQAVAQQISDLEGVTEVAVGFDVLSDDEKGQLQQTLGRGKLPQ
                     GALAKVKNVICVSSGKGGVGKSTMTANLAAALTAEGHPAGALDCDVYGYSIPRMLGVN
                     AKPEVNGERKILPLDSPSGVKVMSIGFFVEDNSAVVWRGPMLHKAIQQFLEDVAWDEL
                     EYLLLDLPPGTGDVSMTLAQLLPQAKFIVVTTPQPAAQSVARRGAQMASKYDLEILGV
                     IENMSGFTTPDGERFTIFGEGGGQLLADELDVPLLGKVPLSEPLREHADSGSPLVTAE
                     PDAPASQAIRQAARGIIASTPQELPVLQATPAAAEPPPVSGTPLPVVQG"
     regulatory      1366..1508
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1565..2065
                     /locus_tag="VFB52_04535"
     CDS             1565..2065
                     /locus_tag="VFB52_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05631.1"
                     /translation="MLSASPALAAPGGVGTTSTSTQSTETSETGPTGKAALVNGRAIA
                     PIDAPPAVKKVIAAANKIRTKPYIYGGGHGRWWDNGYDCSGAVSFALKGGSMIDSPLP
                     SGSMMSWGAAGLGRWITVFANSGHAYAVIAGLRWDTSGTGGSGPRWQEDMRSRAGFVA
                     RHPTGY"
     gene            complement(2165..2434)
                     /locus_tag="VFB52_04540"
     CDS             complement(2165..2434)
                     /locus_tag="VFB52_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZO05632.1"
                     /translation="MTYVIAEPCIGTKDNSCVEVCPVDCIHPTPDEPDYDSVEMLYID
                     ADECIDCDACVEACPVDACFAEDQLPGEWAKYAEINAEYYKSKAA"
     gene            2581..>2974
                     /locus_tag="VFB52_04545"
     CDS             2581..>2974
                     /locus_tag="VFB52_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZO05633.1"
                     /translation="MLLLTGATGSVGSRLLPLLLESGEHVRCLVREPRRLGEHRVDVQ
                     IALGDLGEMSDPYLVRQALRGVNTVIHLAATIRDQPPKRIEELNGLATVRLLRAAERS
                     GVRRFHFFSALDAGGAQRTRFFRAKWVAE"
BASE COUNT          459 a         1055 c          999 g          461 t
ORIGIN      
        1 gcccgacgcc accgcgcgtc cccgccgccg ctcttctccg ctcgctcgcc atgcccgcgc
       61 caaggccgca gccgccccga tctctccccc tccaccgggc cgcgcccgct gccgcgcttc
      121 gcgctttgcg agaatgcgaa ccatggctct tcccacccag gagcagatca ccgagcgcct
      181 gcgcgcggtg atcgaccccg agctccgccg ctcgatcgtc gagctcggca tggtccgctc
      241 gatcctcgtc caggaaagcg gccgggtcga ggtggtggtc tcgctgacca ccgccggctg
      301 cccgatccgc tctcacttcg aacaggcggt cgcccagcag atcagcgacc tcgagggtgt
      361 gaccgaggtc gcggttggtt tcgacgtcct ctccgacgac gagaaagggc agctgcagca
      421 gacgctcggc cgcggcaagc tgccgcaggg cgcgctcgcc aaggtgaaaa acgtgatctg
      481 cgtctcctcc ggcaagggcg gcgtcggcaa gtcgacgatg accgccaacc tggccgcggc
      541 gctgaccgcc gaaggccacc cggccggcgc gctcgactgc gacgtctacg gctactcgat
      601 cccacgcatg ctcggggtca acgccaaacc ggaagtcaac ggcgagcgca agatcctgcc
      661 gctggactcg cccagcgggg tcaaggtgat gtcgatcggc ttcttcgtcg aggacaactc
      721 cgccgtcgtc tggcgcgggc cgatgctgca caaggcgatc cagcagttcc tcgaggacgt
      781 cgcctgggac gagctggagt acctgctgct cgacctgccg ccggggaccg gcgacgtctc
      841 gatgacgctc gcccagctgc tgccgcaggc gaagttcatc gtcgtcacca cgccgcagcc
      901 ggccgcccag tcggtcgccc gccgcggcgc ccagatggcc tcgaaatacg acctcgagat
      961 cctcggcgtg atcgagaaca tgtccggctt caccaccccc gacggtgagc gcttcacgat
     1021 cttcggcgag ggcggcggcc agctcctcgc cgacgaactc gacgtgccgc tgctcggcaa
     1081 ggtgccgctg tcggagccgc tgcgcgagca cgccgactcc ggctcaccgc tggtcaccgc
     1141 cgagcccgac gcccccgctt cgcaggcgat ccgccaggcg gcgcgcggga tcatcgcctc
     1201 gaccccgcag gagctaccgg tgcttcaggc aacgccggcc gcggccgaac cgccgccggt
     1261 cagcggcacc ccgctgccag tcgtccaggg ctagcgagtg caaagcccgg gttccgggcg
     1321 cccagagggg ccgcgcaact tctgtcgcag ccctgtgtta gcttttccaa acaattgcct
     1381 aatccctggc taagagccag ggagcggggg acccaagttt tcggggcgag cccacctctc
     1441 ttggtggggg cgctgctcct tcagcgctag cccgtcagct aactccgcag gccgaagaag
     1501 gaggaactgg atgacgctcg cccgcaaggc ggcttctctg gccgttttat gcagtgccat
     1561 agcagtgctt tccgcgagtc ccgcgctggc cgctcccggc ggcgtcggca cgacctcgac
     1621 ctcaacccag tcgacggaaa cgtcggaaac cgggccgacc ggcaaggcgg cgctggtcaa
     1681 cggccgcgcg atcgccccga tcgacgcgcc gcccgcggtc aagaaggtga tcgcggccgc
     1741 gaacaagatc cgcaccaagc cgtacatcta tggcggcggc cacggtcgct ggtgggacaa
     1801 cggctacgac tgctccggcg cggtcagctt cgccctcaaa ggcggcagca tgatcgacag
     1861 cccgttgccc tccggctcga tgatgagctg gggagcggcg ggtctggggc gctggatcac
     1921 cgtgttcgcc aactccggcc acgcctacgc ggtgatcgcg gggctgcgct gggacacctc
     1981 cgggaccggc ggcagcggac cgcgctggca agaagacatg cgctcccgcg ctggcttcgt
     2041 cgcccgtcat ccgaccggct actagtcagg gaccgctcgc accaggagct tcaaagctcc
     2101 ctggccaggc aagaaaaagg gccgcccagt gggcggccct ttttcgaagc ttcttgccga
     2161 gtggctaggc ggccttgctc ttgtagtact cggcgttgat ctcggcgtat ttagcccact
     2221 cgccgggcag ctggtcctcg gcgaagcagg cgtccaccgg gcaggcctcg acgcaggcgt
     2281 cgcaatcgat gcactcgtcg gcatcgatgt agagcatctc gaccgagtcg taatcgggct
     2341 catccggagt cggatggatg cagtcgacag ggcaaacctc aacgcaagag ttgtccttgg
     2401 tgccgatgca cggctctgcg atcacgtagg tcattcggaa ggcttctcct aagtcggttt
     2461 ccgggtgctg ccggagggcc ccggccaggt gcgagccacc attttaggga tttgcggggc
     2521 gggagcgccc ctggcggggg gtttgcaggg tgacgggcgg cgactcatta gcatcgccgc
     2581 atgttgcttt tgacgggggc gaccggaagc gtcggctcgc ggctgctgcc gctgctgctg
     2641 gagagcggcg agcacgtgcg ctgccttgtg cgtgagccgc gccggctcgg cgagcaccgg
     2701 gtcgacgtgc agatcgcgct cggcgacctc ggcgagatgt ccgaccccta cctggtgcgc
     2761 caggcgctgc gcggggtcaa caccgtgatc cacctggcgg cgacgatccg cgaccagccg
     2821 ccaaagcgga tcgaggagct gaacgggctg gcgacggtgc ggctgctgcg ggcggccgag
     2881 cgcagcggcg tgcggcgctt ccacttcttc agcgcgctcg acgccggcgg cgcccagcgc
     2941 acccgcttct tccgcgccaa gtgggtggcc gagc
//
LOCUS       DASRIG010000166         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2700247, whole genome shotgun sequence.
ACCESSION   DASRIG010000166 DASRIG010000000
VERSION     DASRIG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700247"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFB52_04550"
     CDS             <1..197
                     /locus_tag="VFB52_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZO05634.1"
                     /translation="HRFVVEADNRRHHGTDVAYERDRWRDRELMRAGYATLRVPWQQA
                     ENEAPAVIDAIRRELSRRRP"
     gene            complement(235..951)
                     /locus_tag="VFB52_04555"
     CDS             complement(235..951)
                     /locus_tag="VFB52_04555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZO05635.1"
                     /translation="MRLVKFLAHGGVASRRKAEEIIAKGIVTVDGEVVTDPARGVDAG
                     NDVRVNGSPVSAEATEVWAVHKPAGVVSTAREPGERPAVVELVDSPARLYPVGRLDAD
                     STGLLLLTNDGELANRLTHPRYEVPKTYRARLRRPIADRDLDRLRGGLELEDGPTAPT
                     QVRRQGDREIEIVLREGRNRQVRRMVEAVGNEVVGLRRVAFGPLSLGRLAEGKARKLS
                     ASEVEELRAAGATEPDSPTH"
     gene            complement(948..1517)
                     /gene="scpB"
                     /locus_tag="VFB52_04560"
     CDS             complement(948..1517)
                     /gene="scpB"
                     /locus_tag="VFB52_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZO05636.1"
                     /translation="MSDLARTVEALLFLSAQPVSAADLAEATQATEGQIEEAIELLRE
                     DLGEGKRGVVLREVSGGFTLASDPAAEEAARRLLAKPRTPPLTQAQAETLAIVAYLQP
                     VSRPEIARIRGVSGESAVQTLAERGLIEESGRSRFGAAIFKTSELFERLFGLSGLDQL
                     PDPAQFDPTPEDERELRERLLKAGEQRAE"
     gene            complement(1514..2257)
                     /locus_tag="VFB52_04565"
     CDS             complement(1514..2257)
                     /locus_tag="VFB52_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZO05637.1"
                     /translation="MAVSDLDLDLELDIFAGPFDLLMAVVLREEVSLLDLQLGEVVVA
                     YVEHLEREGELELDVATEFLVLIAALLELKSRLMLPSADDGIEDLAPEEAVEELLARM
                     LEYRRYSQLAERLSQRFESQRGYLYRSAPLPPELRRVEVDAAIAVYDPDLLGSALGDL
                     LTEPEELNLDHIRPTVSLERRLRALRDALGRRTNFDFDDEFGGEDRLTQAVTIFALLE
                     LYRKGEVTWEQSETLGPILVKKTSPGASA"
     gene            complement(2322..3314)
                     /gene="trpS"
                     /locus_tag="VFB52_04570"
     CDS             complement(2322..3314)
                     /gene="trpS"
                     /locus_tag="VFB52_04570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZO05638.1"
                     /translation="MKIFSGIQPTGAKHLGNYIGAIRQYVEAQDRGEPAIFCIVDLHA
                     ISVPYDPVELRERLYDTTAILLAAGLDPARCILFRQSDVREHTELTWLLSAVTAHGDL
                     DRMHQFKEKSGKARELASAALFYYPVLMAADVLAYRATEVPVGDDQRQHVELMREIAR
                     RFNARFGETLVVPELKIPAVGARIMDLQEPENKMSTTGGTEAGTVLVLDEEKTIRKKV
                     GSAVTDSGREIVAAPDKPGVTNLIDILAVARGVGRPEIEAEFADAGYGDFKKAVADGV
                     VELLAPIRERYAELRPDEAALEATLAAGAEKARAIAAPIVAEVRERMGFGPARA"
BASE COUNT          471 a         1208 c         1200 g          499 t
ORIGIN      
        1 accaccgctt cgtcgtcgag gccgacaacc gccggcacca cggcaccgac gtcgcctacg
       61 aacgcgaccg ctggcgcgac cgggaactga tgcgcgcggg ctacgccacc ctgcgcgtgc
      121 cctggcagca agccgaaaac gaagcacccg cggtgatcga cgcgatccgc cgggagttgt
      181 cccgccgccg accctgatgc ggcgagcccg agccagattc gcccacctac ctgcttagtg
      241 ggtgggcgaa tctggctccg tcgcaccggc cgcccttaac tcctcaacct cagacgccga
      301 gagcttccgc gccttgccct cggccagcct tccgagggag agcggcccga acgcgacgcg
      361 cctcagcccc accacctcgt tgccgaccgc ttcgaccatg cggcgcacct gccggttgcg
      421 tccttcgcgg agcacgatct cgatctctcg atcgccttgc cgccgcacct gggtcggcgc
      481 agtcggaccg tcttcgagct ccaacccgcc ccggaggcga tccaggtcgc ggtcggcgat
      541 cggtcggcgc agccgtgccc ggtaggtctt cggcacctcg tagcggggat gagtgaggcg
      601 gttcgccaac tcgccgtcgt tggtcagcag cagcaggccc gtcgagtcgg cgtcgaggcg
      661 gccgaccggg tacaggcgcg ccggcgagtc gaccagctcg acgaccgccg gccgctcccc
      721 cggctccctc gccgtcgaga ccacgcccgc cggcttgtgc acggcccaga cctcggtcgc
      781 ctcggcgctg accgggctgc cgttgacccg cacgtcgttg cccgcgtcca cgccccgcgc
      841 cggatcggtc accacctccc cgtccacggt gacgatcccc ttcgcgatga tctcctccgc
      901 cttgcgccgc gaagcgacgc cgccatgggc gaggaacttg accagcctca ctcggcgcgc
      961 tgttcgccgg ccttgagcag ccgctcgcgc agctctctct cgtcctcggg cgtggggtcg
     1021 aactgcgccg ggtcgggcag ctggtcgagg ccggagaggc cgaagaggcg ctcgaacagc
     1081 tccgaggtct tgaagatcgc ggcgccgaag cgggagcggc cggactcttc gatcaggccg
     1141 cgctcggcca gggtctgcac cgccgactcg ccgctgaccc cgcggatgcg ggcgatctcc
     1201 ggccgcgaga ccggctgcag gtaggcaacg atcgccaggg tctccgcctg ggcctgggtc
     1261 agaggcgggg tgcgcggctt cgccagcagg cgccgggcag cctcctcggc ggccgggtcg
     1321 ctggcgaggg tgaagccacc ggagacctcg cgcaggacga ccccgcgctt gccctcgccg
     1381 aggtcctcgc gcagcagctc gatcgcctcc tcgatctggc cctcggtcgc ctgcgtcgcc
     1441 tcggccagat cggcggcgga gaccggctga gcggagagga agagcagcgc ctcgacggta
     1501 cgggcgaggt cgctcacgcg ctcgcccccg ggctcgtctt cttgaccagg atcgggccga
     1561 gcgtttcgct ctgctcccag gtcacctcgc ccttgcggta gagctccagc agagcgaaga
     1621 tcgtgaccgc ctgggtcagc cggtcctcgc cgccgaactc gtcgtcgaaa tcgaagttgg
     1681 tgcggcggcc gagcgcgtcg cgcagcgccc gcaggcggcg ctccagggag acggtcgggc
     1741 ggatgtggtc gaggttcagc tcctccggtt cggtcagcag gtcgccgagc gcggagccga
     1801 gcaggtcggg gtcgtagacg gcgatcgcgg cgtcgacctc gacccggcgc agctccggcg
     1861 gcagcggcgc cgagcggtag aggtagccgc gctgggactc gaagcgctgc gagagccgct
     1921 cggcgagctg gctgtagcgg cggtactcga gcatccgcgc gagcagctcc tcgaccgcct
     1981 cctccggcgc cagatcctcg atcccgtcgt cggcgctagg cagcatcaag cgcgacttca
     2041 gctccagcag ggcggcgatc aggacgagga attcggtggc gacgtcgagc tccagttcgc
     2101 cctcgcgttc gaggtgctcg acgtaggcga cgacgacctc gccgagctgc aggtcgagca
     2161 ggctgacctc ctcgcgcagg acgaccgcca tcagcaggtc gaacggcccg gcgaagatgt
     2221 cgagttctag atcgaggtcg aggtcggaga ccgccactac ccgcagacta gagcgacgga
     2281 gggacggcga acgtcccgtt tatgccctat ttgcgggcgc tctaggcgcg cgccgggccg
     2341 aaacccatgc gctcgcgcac ttcggcgacg atcggggcgg cgatcgcccg tgccttctcc
     2401 gccccggcgg ccagggtcgc ctccagcgcc gcctcgtcgg ggcgcagctc ggcgtagcgc
     2461 tcgcggatcg gggcgagcag ttcgaccacg ccgtcggcga cggccttctt gaagtcgccg
     2521 tagccggcgt cggcgaactc ggcctcgatc tcggggcggc cgacgccgcg ggcgacggcg
     2581 aggatgtcga tcaggttggt gaccccgggc ttgtcgggag cggcgacgat ctcgcgaccg
     2641 gaatcggtca ccgccgagcc gaccttcttg cggatcgtct tctcctcgtc gaggacgagc
     2701 acggtgccgg cctcggtacc gccggtcgtc gacatcttgt tctccggctc ctgcaggtcc
     2761 atgatccggg cgccgaccgc ggggatcttc agctcgggca cgaccagggt ctcgccgaag
     2821 cgcgcgttga agcggcgggc gatctcccgc atcagctcga cgtgctggcg ctggtcgtcg
     2881 ccgaccggga cctcggtggc gcggtaggcg agcacgtcgg ccgccatcag caccgggtag
     2941 tagaagagcg ccgccgaggc cagctcacgc gctttgccgg acttttcctt gaactggtgc
     3001 atgcggtcga ggtcgccgtg ggcggtgacc gcggagagca gccaggtcag ctcggtgtgc
     3061 tcgcgcacgt cggactggcg gaagaggatg cagcgggccg ggtcgaggcc ggcggcgagc
     3121 aggatcgcgg tcgtgtcgta gaggcgctcg cgcagctcga ccgggtcgta ggggaccgag
     3181 atcgcgtgca ggtcgacgat gcagaagatc gccggttcgc cgcggtcctg cgcctcgacg
     3241 tactggcgga tcgccccgat gtagttgccg aggtgcttcg cccccgtggg ctggatgccg
     3301 ctgaagatct tcatcgggtc gggagcctaa agagcggcgg gccgactacg ccagctcggc
     3361 ggcgccctcg accccggc
//
LOCUS       DASRIG010000167         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1387622, whole genome shotgun sequence.
ACCESSION   DASRIG010000167 DASRIG010000000
VERSION     DASRIG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387622"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFB52_04575"
     CDS             complement(<1..543)
                     /locus_tag="VFB52_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05639.1"
                     /translation="MTRLPKDRKTHLSAKEIASEALRQFDASKTEPSIRSLADALHVA
                     PSAIYHHYPSRAAIVQAAVERVWWEATAKLLEQIPEPFEADPVEVLVETGLASRRAWL
                     THYRLAPYLAASPEVNEFTRDALGLMSGIFERLGLEGEDAAGAFHVYAAFMIGAVLFT
                     ASRKAANEQLAESGEVRFKVE"
     gene            710..2245
                     /locus_tag="VFB52_04580"
     CDS             710..2245
                     /locus_tag="VFB52_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05640.1"
                     /translation="MRARGTVLAIATALVALVVGVAAVGAASRSGPDWQNPELAANPF
                     SNIHNDSYLSDNYRDLGPVKRGRAEVSQIDKVSFTDPATGQPKTTLLGECAAQTFDAQ
                     GNMVTLCTGVPLPSGGGTWTFHRSIVTIDGAGKILAYATFDSSAPDLEQALSDFGGAG
                     YFYLDDQSRPVVAMPDGSIVVYQREDSVLSDVDSWVAARKIPVGGEAGPLAGHPLYAL
                     MPAPSGEIWFTTADGIVGVVRADDQVRWLDLNARGGGEERIANSHPIGRDGSAYVLTT
                     HRIYRVKIGPGGNPRVVWGASYDRGRKQKSGQVSHGSGTSPTLFEMGGETYVAIADNA
                     RRMNVVVYRTAAKLAPGEQRLFAKVRPFGNRVAVSDENSLVWSPGPGGHGTVLFAENN
                     WGNETVTSTVKTKTTKPGVARMLLAPNGRFTVDARNDRVAVPSVVSKASQRSGLLYTY
                     EKRRSGWYLTALRQDDLKQAWTVRVGDGHARYNNYYAALSLDPDGKTVWIGTTLGLTK
                     VVP"
     gene            2328..>4377
                     /locus_tag="VFB52_04585"
     CDS             2328..>4377
                     /locus_tag="VFB52_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05641.1"
                     /translation="MKLATLLVALSALLAGVVAAPASAAPHAGGLDRAFSGDGIALRG
                     FGQEPVPGGAEKAAQQPDGRVLVFLAGWRAGISIGRFWPDGNLDPSFGDGGYLTADLG
                     AAGSVGTDFAVQPDGRIVVAGFARRRSSESGTLFAVARFLPDGAPDPSFGGDGSVVFP
                     GKPRFLRGYPGSVLIQPDGRILLIGQPGSEGRAELEVVRLEPDGSFDRSYGGDGFVSV
                     FLSKTAYLFANDGGGAIAFRDGKLTVALESESGGSDRAYRFNSDGSLDLSFGGDGRVD
                     QELLVGRKSDLAVDAAGRTVATAEYGRVIRLLPDGSLDPSFGDAGIVRLGDGFEARAV
                     SALADGRLALAGVANEHPEPGPREAFVARLDEAGRPDPGFGGGDGVASTDLVAGSEDE
                     ATAMLMGADGGVLIAGSSTPTEGDGWSSRRIMLARYGPAGDLDLGFGSGGVAIAQARM
                     LAADAVFGVAAGPRGTTVATGRAGGGIAVVRYRRDGEPDPRFGDRGAVRLAVTGSARG
                     EQGESLAVLPGGGLVVGTTSADGGALLRLRPGGALDPGFGRGGVVRTEGIRAVVDLLR
                     LADGKILAVGIEGSERGVLLRYLPGGSLDRDFGDGGRVRFGEVDGKFGGRVDASLAVD
                     RRGRILIAAPDFNGGRVQQFSPNGEPVKSFGRHRDGRRDQGHLGRVIAVAVGRDGKIA
                     V"
BASE COUNT          616 a         1557 c         1560 g          644 t
ORIGIN      
        1 ctcgaccttg aagcggacct caccggactc ggcgagctgc tcgttggcgg ctttgcggga
       61 agcggtgaag agcaccgcgc cgatcatgaa ggcggcgtag acgtggaagg cgccggcggc
      121 gtcctcgccc tcgagcccga gcctctcgaa gatccccgac atcaggccga gcgcgtcgcg
      181 ggtgaactcg ttgacctcgg ggcttgccgc caggtaggga gcgaggcggt agtgggtgag
      241 ccaggcacgg cggctggcga gcccggtctc gaccaggacc tcgaccgggt cggcctcgaa
      301 cggctcgggg atctgctcga gcagtttcgc ggtcgcttcc caccagaccc gctcgaccgc
      361 ggcctggacg atcgccgcgc gcgaggggta gtggtggtag atcgccgagg gggcgacgtg
      421 gagggcgtcg gcgaggctgc ggatgctcgg ctcggtcttg ctggcgtcga actggcgcag
      481 cgcctcgctc gcgatctcct tcgcgctgag atgcgtcttg cgatctttgg gaagcctcgt
      541 caccgcagag aacctgtggc cagcctagag cgcaaggcag gctcggcgcg cccctcccgt
      601 gtatacggcg gttcgcaacg ctaaagaatc tcgctctggt tgccgaacta cattcgtagt
      661 gcgtgtgcgg cacccacccc cgaccagagc ccgcagagga gacgagcaga tgagagcacg
      721 tggaactgtc ttggcgatcg cgaccgccct ggtcgccctg gtggtcggcg tcgccgcggt
      781 cggcgccgcc agccgctccg gcccggactg gcagaacccg gagctggcgg cgaacccgtt
      841 cagcaacatc cacaacgact catacctctc cgacaactac cgcgacctcg ggccggtcaa
      901 gcggggcagg gcggaggtct cccagatcga caaggtcagc ttcaccgatc ccgccaccgg
      961 ccagccgaag acgaccctgc tcggggagtg cgcggcccag accttcgacg cccagggcaa
     1021 catggtcacg ctctgcaccg gtgtgcccct gcccagcggc ggcggtacct ggaccttcca
     1081 ccgcagcatc gtcacgatcg acggcgccgg caagatcctc gcctacgcga cgttcgacag
     1141 cagcgccccg gacctcgaac aggcgctcag cgacttcggc ggcgccggct acttctacct
     1201 cgacgaccaa tcgcgcccgg tggtggcgat gcccgacggc agcatcgtcg tctaccagcg
     1261 cgaggactct gtgctcagcg acgtcgacag ctgggtcgcg gcgcgcaaga tcccggttgg
     1321 cggcgaggcc gggccgctgg ccgggcaccc gctgtacgcg ctgatgccgg cgccgagcgg
     1381 cgagatctgg ttcaccaccg ccgacgggat cgtcggcgtg gtcagggccg atgaccaggt
     1441 gcgctggctc gacctgaacg cccgcggcgg cggcgaagag cggatcgcca actcgcaccc
     1501 gatcggtcgc gacggctccg cctacgtgct caccacccac cggatctacc gggtcaagat
     1561 cggccccggc ggcaacccgc gggtggtctg gggcgcgtcc tacgaccgcg gccgcaagca
     1621 gaagtccggc caggtcagcc acggctccgg cacctcgccg accctgttcg agatgggcgg
     1681 cgaaacctac gtcgcgatcg ccgacaacgc ccgtcgcatg aacgtcgtcg tctaccgcac
     1741 cgcggcgaag ctggcgcccg gagagcagcg cctgttcgcg aaggtgcgcc cgttcggcaa
     1801 ccgggttgcg gtcagcgacg agaactcgct ggtctggtcg cccggccccg gcggccacgg
     1861 caccgtgctc ttcgccgaga acaactgggg caacgagacc gtcacctcga ccgtgaagac
     1921 gaagacgacc aaacccggcg tcgcccgcat gctgctggcg ccgaacggcc gcttcaccgt
     1981 cgacgcccgc aacgaccgcg tcgccgtgcc ctcggtggtc tcgaaggcga gccagcgcag
     2041 cggcctgctc tacacctacg agaagcggcg cagcggctgg tacctgaccg ccctgcgcca
     2101 ggacgacctg aagcaggcct ggacggtccg ggtcggcgac ggccacgccc gctacaacaa
     2161 ctactacgcg gccctctcgc tcgatcccga cggcaagacg gtctggatcg gcacgacgct
     2221 ggggctgacc aaggtcgtgc cgtaggcccg gaccgggcca aacacggctc gccccagggc
     2281 gccactggcc cggtcacgcc ggcgcggtgc tgcgaccatg cccgtcgatg aagctcgcaa
     2341 ccctgctcgt cgcgcttagc gcgttgctcg ccggtgtcgt cgcggccccg gcttcggcgg
     2401 ccccgcacgc cggcggcctc gaccgcgcgt tctccggcga cgggatcgcg ctgcgcgggt
     2461 tcgggcagga acccgtcccc ggcggcgccg aaaaggcggc gcagcagccc gacggcaggg
     2521 tgctggtctt cctcgccggc tggcgggcgg gcatctcgat cggccgcttc tggcccgacg
     2581 ggaatctcga cccgagtttt ggcgacggcg gctacctgac tgctgacctc ggcgcagcgg
     2641 gctcggtcgg caccgatttc gccgttcagc ccgacggcag gatcgtcgtc gccggattcg
     2701 cccggcgccg cagctctgag tcgggcactc tgtttgcggt tgcgcggttc cttcccgacg
     2761 gcgcaccgga ccccagcttc ggcggcgacg gcagcgtcgt tttcccgggc aagccgcgct
     2821 tcctccgcgg ctaccccggt tccgtcctga tccagcccga cggccggatc ctcctgatcg
     2881 ggcagccggg ctcggaaggg cgagcggagc tggaggtcgt gcggctcgag cccgatggca
     2941 gcttcgaccg ctcctatggc ggcgacggct tcgtctccgt cttcctctcc aagaccgcct
     3001 atctctttgc caacgacggc ggtggcgcga tcgcctttcg cgatggcaag ctgaccgtcg
     3061 cgctcgagtc cgaatccggc ggctctgatc gcgcctaccg cttcaattcc gacggctcgc
     3121 tcgatctgag cttcggtggc gatggccggg tcgaccagga actgctcgtc ggccgcaagt
     3181 ctgacctcgc ggtcgacgct gcggggcgca cggtggccac cgccgagtac ggccgcgtga
     3241 tccggctgct gcccgacggc tcgctggacc ccagcttcgg cgacgcgggg atagtgcgct
     3301 tgggcgacgg cttcgaagcg cgggcggttt ccgcgctcgc cgatggccgt ctagcgctcg
     3361 ccggtgtggc caacgaacat ccggagcccg ggccgcgcga agccttcgtg gcgcgcctcg
     3421 acgaggccgg gcggcctgac cccggcttcg gcggtggcga cggggtcgcc tccaccgatc
     3481 tggtcgccgg ctccgaggac gaagcgaccg cgatgctgat gggtgccgac ggcggcgttc
     3541 tgatcgcggg gtcctcgacg ccgaccgagg gcgacggctg gtcctcaaga cggatcatgc
     3601 tggcccgcta cgggccggcc ggcgatctcg acctcggctt cggcagtggc ggcgtcgcaa
     3661 ttgcccaggc gcgcatgctg gctgccgacg cggtcttcgg cgttgccgcc ggcccgcgcg
     3721 ggacgacggt cgccaccggt cgcgccgggg gcggaatcgc cgtggtcagg taccgccggg
     3781 acggggagcc ggatccgcgc ttcggcgatc gcggggcggt ccggctggcg gtcaccggct
     3841 ccgcgcgcgg agagcagggc gagagcctgg ccgtcctacc cggcggtggc ctagtggtcg
     3901 gcaccacgtc ggccgacggc ggggcgctgt tgcgcctgcg ccccggcggt gcactcgatc
     3961 cgggcttcgg ccggggtggc gtggtccgca ccgagggaat ccgcgccgtc gtcgacctgc
     4021 tccgcctcgc ggacggcaag atcctcgccg tcggaatcga aggcagcgag cgcggggttc
     4081 tcctgcgtta cctgcctgga ggctcgctcg atcgcgactt cggcgacggc ggcagggtgc
     4141 ggttcggaga agtcgacgga aagttcggcg ggcgtgtcga cgcctcgctt gcggtcgaca
     4201 ggcgcgggcg cattctcatc gcggcaccgg atttcaacgg aggtcgcgtc cagcagttca
     4261 gccccaacgg cgagccggtc aagagcttcg gcaggcatcg tgacggtcgc cgggaccagg
     4321 ggcacctcgg cagggtcatc gcggtcgcgg tcggccgcga cggcaagatc gcggtcg
//
LOCUS       DASRIG010000168         4931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1388851, whole genome shotgun sequence.
ACCESSION   DASRIG010000168 DASRIG010000000
VERSION     DASRIG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388851"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFB52_04590"
     CDS             <1..318
                     /locus_tag="VFB52_04590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZO05642.1"
                     /translation="FHYFFARKLMFVRYARAFVIFPGGFGTLDEMFESLTLIQTHRIK
                     NFPTILVDSAHWRPLLDWIDTSLEDTNMISPGDKELLIVVDRPEEVCDYVLRAKEAQT
                     ATA"
     gene            507..1436
                     /locus_tag="VFB52_04595"
     CDS             507..1436
                     /locus_tag="VFB52_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05643.1"
                     /translation="MLVEAGDLGELLSMAARARSSYRFRMRRSDGTIARYRGGKNIET
                     LVDGEWISVLLPSGFWDERDWLPVNDDEQLAHHALQCLTPDLDRVGLRWSPFWARFAR
                     SFLGRLAVPPLFGGAPRAPDERDTMRCRAVLLSCFSRVSDEEMEELHDQALRGYELQR
                     ERNATVEQRANFFLGAAGLSSTLVLANASILLGAGDGVLGSPWRELSAAAFAVASVFA
                     IVAGLRALQAVMLAFQRVSPSGVGSILEADSATGSRRARRNHASFLLLAQERELRVHE
                     WKLARLGEARRWFGGAVLGVVVVTAFVLVEVLR"
     gene            complement(1446..3062)
                     /gene="purH"
                     /locus_tag="VFB52_04600"
     CDS             complement(1446..3062)
                     /gene="purH"
                     /locus_tag="VFB52_04600"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZO05644.1"
                     /translation="MSEARADTANETTGDGGIRVRRALVSVSDKTGIADFAKSLAALG
                     VEILSTGGTASALREAGIAVTDVSEFTGQEEILDGRVKTLHPRLHAALLARRDDPDHM
                     ATLEREGIEPIDVVCVNLYPFEQTIAAYDVAPEVAIENIDIGGPTMIRAAAKNHEGVA
                     VIVKPESYDAVCAELEESGGVISASTRHWLANEAFAQTARYDAAISRWFSTSYEDFPE
                     HLAIAYEKVIDLSYGENPHQRAALYAEAGVRSHILSRVSKLHGKALSFNNVLDLDSAR
                     GLVEDFQAPACVIVKHNNPCGVAVGKDALEAYLKALACDPVSAYGGVIAFNRRVSAEL
                     AEELHKNFVEVLYAPGYEAGALPVLQQKESIRILDEEERRQPDPIERDVKKVLGGLLI
                     QDRDGDPEPREIMEVVTDTQPSEQQWNDMLFAWTVSRRVRSNAIVIAKDGATLGIGAG
                     QMSRVDSVRLAVEKCRDARGEEADALLAGSAVASDAFFPFADGPELAIQAGSNSVIQP
                     GGSKRDAEVIAACDEGGIAMVFTKHRHFRH"
     gene            complement(3055..3672)
                     /gene="purN"
                     /locus_tag="VFB52_04605"
     CDS             complement(3055..3672)
                     /gene="purN"
                     /locus_tag="VFB52_04605"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZO05645.1"
                     /translation="MSAFRIVVLASGSGTNLQAILDQLHGREGVEVVGVGTDKPGAKA
                     LERAVAAGVETAAFPREAYADRLGRDAAMADWVQARGADLVVLAGYMQLLSEPFVTRF
                     RGRIVNIHPALLPAFPGLDAIGQALEAGVEKTGVTVHFVDEGVDTGPVIVQRPVPVPA
                     GRERQVLEAAVHSVEHQLYPEAIRMIAGGRVRIDPDDPSVVLIDE"
     gene            3726..4157
                     /locus_tag="VFB52_04610"
     CDS             3726..4157
                     /locus_tag="VFB52_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZO05646.1"
                     /translation="MKRAEALQPLSREHQGALAVAKALSEAEDREAAARTLLEFWRAD
                     GRRHFRIEEEVLLPGWAFVAEVDWAGAERMLREHLEIRRQVLRTEAGEIDLDELRALG
                     RLLHDHVRYEERQLFPEVEAALGKADLDRLAAAIEVAEAAG"
     gene            complement(4175..>4931)
                     /locus_tag="VFB52_04615"
     CDS             complement(4175..>4931)
                     /locus_tag="VFB52_04615"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZO05647.1"
                     /translation="GEMVSLTERGLETRQVVKAERPATCVFEHIYFSRPDSRLEGKVL
                     QQVRGRMGEILWREAPVEADLVISVPDSGNPAAAGFARASGLPRDDGLIKNRYVARTF
                     IQPGQELRKHGLRLKFNPLPEIVAGQRIVVVDDSIVRGNTTRQIVGMLRDAGAAEVHL
                     RISAPPIRNPCHYGVDMSTREEMIAHKRSVQEIADELDADSLAYLSMAGVYEAIGGSP
                     DEHCDACFSGHYPLGDPEDANGKFALETIASGA"
BASE COUNT          728 a         1664 c         1709 g          830 t
ORIGIN      
        1 ttccactact tcttcgcccg caagctgatg ttcgtccgct acgcccgcgc cttcgtgatc
       61 ttccccggcg gcttcggcac cctcgacgag atgttcgagt ccctcaccct gatccagacc
      121 caccggatca agaacttccc gacgatcctc gtcgactccg cccactggcg ccccctgctc
      181 gactggatcg acacctccct cgaggacacc aacatgatct ctcccggcga caaagagctc
      241 ctgatcgtcg tcgaccgccc cgaggaagtc tgcgactacg tgctccgcgc caaagaggcc
      301 caaaccgcca cggcctagaa cgcaaacggc ccgccgaagc gggccgtttg agaagtggag
      361 ctgccgtcga acagacgact ccgtcctcag tgcgcatgtg tgatgcgcac gctgagtcaa
      421 ttgtgtgacc ccttcgtgca gaaaacgcca gcaggagcaa taccccaact gttgactagt
      481 actaaggcag tccttagcat ggcgcggtgc tcgtcgaggc aggggatttg ggtgagctgt
      541 tgtcgatggc ggcgcgagcc cgatcgagct acaggttccg aatgcggcgc agcgatggca
      601 cgatcgcccg ctatagaggt ggcaagaaca tcgagaccct cgtcgatggc gagtggatct
      661 cggtcttgct tccctccggc ttttgggacg aacgggactg gctcccagtc aacgacgacg
      721 aacagctcgc acaccacgcc ctgcagtgcc tgaccccgga tttggaccgg gtggggcttc
      781 ggtggtcgcc cttttgggcg cggttcgctc gctcttttct ggggcgcttg gcagttccgc
      841 cactctttgg tggcgcccct cgggctccgg atgaacgaga cacgatgcgc tgccgcgcag
      901 tccttctcag ttgcttctcg agagtgagcg acgaggagat ggaggaactc cacgaccagg
      961 ccctacgagg ttatgaactt cagcgagagc gcaacgcgac cgtggagcaa cgggccaact
     1021 tcttcctcgg tgccgctggc ctctcgagca cgctggtgct ggccaacgcg agcatcttgc
     1081 tcggggcggg agatggagtc ctcggatctc cttggcgtga gttaagcgcc gccgcgttcg
     1141 cagtggcgag cgtattcgcg atcgttgccg ggctcagggc gcttcaggca gtgatgctgg
     1201 cgttccagcg ggtctctcca agcggcgtcg ggtcgatcct ggaggccgac tccgcaacag
     1261 ggtcccggcg tgcgaggcgg aaccacgcga gcttcctgct gttagcccag gagcgcgaac
     1321 tgcgcgtcca tgaatggaag ctcgcccggc tcggagaagc acgcaggtgg tttggcggcg
     1381 ccgtactcgg agtggtcgtc gtcaccgcgt ttgtgctcgt ggaagtgctc cggtgaggcc
     1441 ggaagctagt gccggaagtg gcggtgcttg gtgaagacca tcgcgatgcc gccctcgtcg
     1501 caggcggcga ttacctcggc gtcgcgtttg gagccgccgg gctggatcac cgagttggag
     1561 ccggcctgga tcgccagctc ggggccgtcg gcgaagggga agaaggcgtc ggaggcgacg
     1621 gcggagccgg ccaggagggc gtcggcttcc tcaccgcggg cgtcgcggca cttctcgacc
     1681 gcgaggcgga ccgagtcgac gcggctcatc tgacccgctc cgatgccgag ggtggcgccg
     1741 tccttggcga tcacgatcgc gttcgagcgg acgcggcggc tgacggtcca ggcgaacagc
     1801 atgtcgttcc actgctgctc gctcggctgg gtgtcggtca ccacctccat gatctcgcgc
     1861 ggctcgggat cgccgtcgcg gtcttggatc aaaaggccgc cgagcacctt cttgacgtcg
     1921 cgttcgatcg ggtcgggctg gcggcgctcc tcctcgtcga ggatccggat cgactccttc
     1981 tgctgcagaa cgggcagcgc gccggcttcg tagccgggcg cgtagaggac ctcgacgaag
     2041 ttcttgtgca gctcctcggc cagctccgcc gaaacccggc ggttgaaggc gatcacgccg
     2101 ccgtaggcgg agaccggatc gcaggcgagt gccttcaggt aggcctcgag cgcgtccttg
     2161 ccgaccgcca ccccgcaggg gttgttgtgc ttgacgatca cgcaggccgg cgcctggaag
     2221 tcttcgacca ggccgcgggc tgagtcgagg tcgagcacgt tgttgaacga gagcgccttg
     2281 ccgtgcagct tcgagacccg cgagaggatg tggctgcgca cgcccgcttc cgcgtagagc
     2341 gccgcccgct ggtgcgggtt ctcaccgtag gagaggtcga tcaccttctc gtaggcgatc
     2401 gccaggtgtt cggggaagtc ctcgtaggag gtcgagaacc agcggctgat cgcggcgtcg
     2461 tagcgcgcgg tctgggcgaa ggcctcgttg gcgagccagt gccgggtcga ggccgagatc
     2521 acgccgccgc tctcctccag ctcggcgcag accgcgtcgt aggactccgg cttgacgatc
     2581 accgcgacgc cctcgtggtt cttcgccgcc gcccggatca tcgtcggccc gccgatgtcg
     2641 atgttctcga tcgcgacctc cggggccacg tcgtaggcgg cgatcgtctg ctcgaacggg
     2701 tagaggttga cgcagaccac gtcgatcggc tcgatcccct cgcgctccag cgtcgccatg
     2761 tgatcagggt cgtcgcgtcg cgccagcagc gccgcgtgca gccgcgggtg cagcgtcttc
     2821 acccggccgt cgaggatctc ttcctggccg gtgaactcgg agacgtcggt gaccgcgatc
     2881 ccggcctcgc gcagggcgct cgccgtcccc ccggtcgaga ggatctcgac cccgagcgcg
     2941 gcgaggcttt tggcgaagtc cgcgatcccg gtcttgtcgg agaccgagac cagggcccgg
     3001 cgcacccgaa tcccgccgtc tccggtggtc tcgttcgcgg tatctgccct tgcctcactc
     3061 atcgatcagg acgacgctgg ggtcgtcggg gtcgatccta acccggcccc ccgcgatcat
     3121 gcggatcgcc tccgggtaga gctggtgctc gacactgtgc acggccgcct ccaacacctg
     3181 ccgctcgcgc cccgccggca ccggcaccgg ccgctgcacg atcaccggcc cggtatcgac
     3241 cccctcgtcg acgaagtgca ccgtgacccc ggttttctcg accccggcct cgagcgcctg
     3301 gccgatcgcg tcgagcccgg ggaaggccgg cagcagggcg gggtggatgt tgacgatgcg
     3361 cccacggaag cgggtcacga acggttcgct gagcagctgc atgtagccgg cgagcacgac
     3421 caggtcggcg ccgcgcgcct gcacccagtc ggccatcgcc gcgtcgcgcc cgagccggtc
     3481 ggcgtaggcc tcgcggggga aagccgcggt ctcgaccccg gccgcgaccg cccgctccag
     3541 cgccttggcg ccgggcttgt cggtcccgac cccgaccacc tcgaccccct cgcgcccgtg
     3601 cagctggtcg aggatcgcct gcaggttggt gccgctgccg gaggcgagga cgacgatgcg
     3661 gaaggcgctc accgccgcat cttcgcgcac ctgccgggat gccggcggcg gcctatcctg
     3721 gagagatgaa acgagccgag gcactgcagc cgctctcgcg tgagcaccag ggcgcgctcg
     3781 ccgtcgccaa ggccctgagc gaggccgagg atcgcgaggc ggccgcgcgc accctgctcg
     3841 agttctggcg tgccgacggc cgtcggcact tccgcatcga ggaggaggtg ctgctgccgg
     3901 gctgggcgtt cgtcgccgag gtcgactggg ccggagccga gcgaatgctg cgcgagcacc
     3961 tcgagatccg tcgccaggtg cttcggaccg aagcggggga gatcgacctt gacgagctgc
     4021 gtgcgctcgg ccgactcctc cacgaccacg tccgctacga ggagcgccag ttgttcccgg
     4081 aggtcgaagc cgcgctcggc aaagcggacc tcgaccggct cgccgccgcg atcgaggtag
     4141 ccgaggcggc cgggtgagcg agcactacct ctgatcaggc gccgctggcg atcgtctcga
     4201 gcgcgaactt gccgttcgcg tcttcggggt cgccgagcgg gtagtggccg ctgaagcagg
     4261 cgtcgcagtg ttcgtcgggg ctgccgccga tcgcctcgta gacgcccgcc atcgagaggt
     4321 aggcgagcga gtcggcgtcg agctcgtcgg cgatctcctg gaccgagcgc ttgtgggcga
     4381 tcatctcctc gcgggtcgac atgtcgacgc cgtagtggca ggggttgcgg atcggtgggg
     4441 ccgagatccg caagtgcacc tcggcggcgc cggcgtcgcg cagcatgccg acgatctggc
     4501 gagtcgtgtt gccgcgcacg atcgagtcgt cgacgacgac gatcctttgg ccggcgacga
     4561 tctccggcag cgggttgaac ttcagccgca ggccgtgctt gcgcagctcc tggccgggct
     4621 ggatgaaggt ccgagccacg tagcggttct tgattaggcc gtcgtcgcgg ggcagccccg
     4681 aggcgcgggc gaagccggcg gcggccgggt tgcctgagtc cggcaccgag atcaccaggt
     4741 cggcttcgac cggcgcctcg cgccagagga tctcgcccat ccggccgcgc acctgctgca
     4801 ggactttgcc ctcgagccgg ctgtcggggc gggagaagta gatgtgctcg aagacgcagg
     4861 tcgcgggccg ctcggccttc accacttggc gagtctcgag gccgcgctcg gtcagcgaga
     4921 ccatctcgcc c
//
LOCUS       DASRIG010000169         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2951658, whole genome shotgun sequence.
ACCESSION   DASRIG010000169 DASRIG010000000
VERSION     DASRIG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951658"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFB52_04620"
     CDS             <1..598
                     /locus_tag="VFB52_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05648.1"
                     /translation="MLPDLGLVVCTSRSTVAQRVKGLRLGVDDWMTKPCHPEEAMARL
                     EAAARRRRRGRPAAERGPIVTGELEIRSDRYQAFVDGDSLDLTRREFELLHLLAEARG
                     QVLEREAIYQRVWGYAMAHGDRSVDVFIRKLRQKLEKRSPSWAYIHTHFGVGYRFDPE
                     PTVDMIPDAPPVEATPAAAPEHPDPAASQVVHTSFTSR"
     gene            640..969
                     /locus_tag="VFB52_04625"
     CDS             640..969
                     /locus_tag="VFB52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05649.1"
                     /translation="MAIGTGNSSRLEAGHLEIRPGEHAALVDGRPLSLTVRELQLLIT
                     LAEHPQRIMTRDELFAAVWHGRPRRDDRSVDVYVSRLRAKLGEALPGMTLIHTHNGIG
                     YRFSPDG"
     gene            1124..2245
                     /gene="pstS"
                     /locus_tag="VFB52_04630"
     CDS             1124..2245
                     /gene="pstS"
                     /locus_tag="VFB52_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZO05650.1"
                     /translation="MSRTRWLAVIVFSAILALGVAACGGSDDDSSGGGGSTSGGGESA
                     EVSGEIAGAGASSQEAAQEAWIAEFENANGGATISYDPVGSGGGREQFIAGGVAYAGS
                     DAAISEEEGELKKAIARCEPGELIEIPAYVSPIAIIYNLPEVEELQLDPETLAKIFNQ
                     EIDNWNDPAIAKTNPDVELPDTRITPVNRSDESGTTENFTEYLSEVVPTVWDHEVSGD
                     WPVKGGEAAQGTSGVVEAVTAGEGAIGYADASQADELGKAKIEVGSEFVEPSPEAAAA
                     ILDESPEDKADAPGKYMFPFTLDRKTEAEGTYPIVLVSYLLACTKYDSAEEAAVVKAF
                     LSYVISTEGQEAAATNAGSAPLSAALTEKITPAVEAIEG"
     gene            2250..>3125
                     /gene="pstC"
                     /locus_tag="VFB52_04635"
     CDS             2250..>3125
                     /gene="pstC"
                     /locus_tag="VFB52_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZO05651.1"
                     /translation="MASVDTTTNAGQLLAAASGGRRRVGDRVFSNVAVIAAVTILALL
                     AGVAIFLVSEGLPALTDPDATPFIDYVAPLAFGTVLAAAFAVVIAVPLAVAAALFITH
                     IAPQRVALTLGYLVDLLAAIPSIIYGLWGIFVLGPAAVPVMQWLEEKLGFIPLFEGPA
                     SATGRTMFVAGLVLAVMILPIVSAVAREVFSQTPRKLQEAALSMGATRWEMIRMTVLP
                     FGKSSVISGAMLGLGRALGETMAVTIILSVSGIVTFNLISSANPSTIAANIALDFPES
                     SGLAVNTLIATGLVLF"
BASE COUNT          531 a         1096 c          992 g          506 t
ORIGIN      
        1 gatgctgccc gacctcggcc tagtcgtctg caccagccgc tccaccgtcg ctcagcgggt
       61 gaaggggctg cggctcgggg tcgacgactg gatgaccaag ccctgccacc cggaggaggc
      121 gatggcccgg ctcgaagccg ccgcccgccg ccgccgtcgc ggccgcccgg ccgccgagcg
      181 cggcccgatc gtcaccggcg agctggaaat ccgctccgac cgctaccagg cgttcgtcga
      241 cggcgacagc ctcgacctca cccggcgcga gttcgagctg ctccacctgc tcgccgaggc
      301 gcgcggccag gtgctcgagc gcgaggcgat ctaccagcgg gtctggggct acgcgatggc
      361 ccacggcgac cgctcggtcg acgtcttcat ccgcaagctg cgccagaagc tcgagaagcg
      421 ctcgccgagc tgggcctaca tccacaccca cttcggcgtc ggctatcgct tcgaccccga
      481 gccgaccgtc gacatgattc ccgacgctcc gccggtcgag gccacccccg cggccgctcc
      541 ggagcatccg gaccccgcgg cttcacaagt cgttcacacg agtttcactt cgagataacc
      601 agcgctcagg catgccaccg gcatgcttcc tgagcgaaca tggcgatcgg aactgggaac
      661 tcctcgcggc ttgaggccgg gcacctcgaa atcaggcccg gggagcacgc ggccctggtt
      721 gacggccgcc cgctcagcct caccgtgcgc gagctgcagc tgctgatcac gctggccgaa
      781 catccgcagc ggatcatgac cagggacgag cttttcgccg ccgtctggca cggccggccg
      841 cgccgggacg accgctccgt ggacgtctac gtgagccgcc tgcgagcgaa gctcggtgag
      901 gcgctgccgg ggatgacgct gatccacacc cacaacggga tcggttaccg gttctcgcct
      961 gacggctgag aactcatcct ctcggccgcc ttttacatct tttttacatc ttcgacacag
     1021 cggagtaaca aattgctgtg agggccacgc gaggctggct gaggcctcga cgccacccct
     1081 gaattgtcga tggccgaaac gcgaaaacaa ctaagggaga ctgatgtcta ggactagatg
     1141 gcttgcggtc attgtcttct ccgcgatctt ggcgctcggt gttgccgcct gcggtggcag
     1201 cgatgacgac agcagcggcg ggggcggttc gacctccggc ggtggcgaat ccgccgaagt
     1261 ttccggcgaa atcgctggtg ccggcgcgag ctcgcaggag gccgcgcagg aagcttggat
     1321 cgccgaattc gagaacgcca acggcggcgc gaccatttct tacgacccgg tcggctccgg
     1381 cggcggtcgt gagcagttca tcgccggcgg tgtcgcctac gccggttctg acgctgcgat
     1441 cagcgaagag gaaggcgaac tgaaaaaagc aatcgcgcgt tgcgagcccg gggaactgat
     1501 tgagatcccg gcctacgtct cgccgatcgc gatcatttac aacctccccg aagtcgaaga
     1561 actccagctc gatcccgaaa ccctggcgaa gatcttcaac caggaaatcg acaactggaa
     1621 cgacccggcg atcgccaaaa ccaaccccga tgtggagctg ccggacaccc gcatcacccc
     1681 ggtgaaccgc tcggacgagt ccgggaccac ggagaacttc accgaatacc tctcggaggt
     1741 cgtgcccacg gtctgggatc acgaagtctc cggtgactgg ccggtcaaag gcggcgaggc
     1801 cgcgcagggc acctccggcg tggtcgaagc ggttaccgcc ggcgaaggcg cgatcggtta
     1861 cgccgatgcc agccaggccg acgaactcgg gaaagccaaa atcgaggtcg gctcggaatt
     1921 cgtcgaaccg tcgccggaag ccgcggccgc gatcctcgac gagtcgcccg aagacaaagc
     1981 ggacgcgccc gggaaataca tgttcccgtt cacgctcgac cgcaaaaccg aagccgaggg
     2041 cacctacccg atcgtgctgg tcagctacct gctcgcctgc acgaaatacg actcggctga
     2101 ggaagccgcc gtcgtcaagg cgttcctctc gtacgtgatc agcaccgaag gccaggaagc
     2161 ggccgccacc aacgctggct ccgctcccct gtcggcggcg ctgacggaaa agatcactcc
     2221 cgctgtggaa gcgatcgaag ggtagttagt tggcatcggt cgacaccacg accaatgcgg
     2281 gtcagctgct cgcggcggcg tccggaggac gccgccgcgt cggcgaccgt gtcttttcca
     2341 acgtcgcggt aatcgcggcg gtaacgatcc tggcgctgct tgccggggtc gcgatcttcc
     2401 tggtcagcga ggggctgccg gcgctcaccg accccgacgc cacgcccttc atcgactacg
     2461 tggcgccgct cgccttcggg accgtgctgg ccgccgcctt cgcggtcgtc atcgcggtcc
     2521 cgctggcggt cgccgcggcg ctcttcatca cccacatcgc gccgcagagg gtggcgctga
     2581 cgctcggcta cctggtcgac ctgctggccg cgatcccgag catcatctac ggcctctggg
     2641 gcatcttcgt gctcggcccg gccgctgtcc cggtgatgca gtggctggaa gaaaagcttg
     2701 gcttcatccc cctcttcgag ggccccgcat cggccaccgg ccggaccatg ttcgtcgccg
     2761 gcctggtgct ggcggtgatg atcctgccga tcgtctccgc ggtcgcccgc gaggtcttct
     2821 cgcagacccc gcgcaaactc caggaagcgg cgctctcgat gggggcgacg cgctgggaga
     2881 tgatccggat gaccgtcctc ccgttcggca aatcgtcggt gatctccggc gcgatgctcg
     2941 gcctcggccg ggcgctgggc gagacgatgg cggtgacgat catcctctca gtctccggca
     3001 tcgtcacctt caacctgatc agctcggcga acccctcgac gatcgccgcc aacatcgccc
     3061 tcgacttccc cgagtcctcc gggctggcgg tgaacacgct gatcgccacc ggcctcgtcc
     3121 tcttc
//
LOCUS       DASRIG010000170         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3475788, whole genome shotgun sequence.
ACCESSION   DASRIG010000170 DASRIG010000000
VERSION     DASRIG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475788"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFB52_04640"
     CDS             <1..421
                     /locus_tag="VFB52_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO05652.1"
                     /translation="LVEFQLDATRGSGGLSGEDLVEAGRRAIERETLITYSDFKKGVG
                     SLATIATTAPFIGLFGTVVGIMFAFQEISGAGNATLQVVGPHIAEALFATAIGLVAAV
                     PAVMAYNYFTRRIRVQRSEMETFEQDYLNIIKRHFLH"
     gene            431..871
                     /locus_tag="VFB52_04645"
     CDS             431..871
                     /locus_tag="VFB52_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZO05653.1"
                     /translation="MGMRTGGGDELNSEINVTPMVDVMLVLLIIFMITAPMMNTGVDL
                     DLPQVNAQNIEDPAGKLVLSIDPARKLYLGGTQVTWKDLEVKLSSNERVKKEQQLYIE
                     ADQSLPYGVVVTAMALAKKAGVAKVMMLTDPTEQMKVEELDVPR"
     gene            887..1603
                     /locus_tag="VFB52_04650"
     CDS             887..1603
                     /locus_tag="VFB52_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HZO05654.1"
                     /translation="MRDPLHLAASLGTAATTVALIGLAVFVDWAPAALAGEGPNLDEM
                     EAIEASIAYKKAEPPKQPQKQKRAPVPEEIEGVSRDENKTPVEPKKDDKRKPDENFED
                     QYKKFQRKDDEDLEAGKPTDENVGSFDGSEFGFAEETKGDPYFQKLVLDLREGWEYPE
                     ILSDVGVPVACIRLAADGKILDTNFRERSNNAELDDSVERALAALKKKRGQEPVPVPT
                     HLLKWTTKWTCFRLKPSAAR"
     gene            1564..>2394
                     /locus_tag="VFB52_04655"
     CDS             1564..>2394
                     /locus_tag="VFB52_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05655.1"
                     /translation="MDLLSTQALRRALAALAVLGLAAVADAPARADDPSGVIIDVSGA
                     RRGLYPLAIPVAVNSDGAAAREVADIASFDMQVAGFFKVLDPASFLADLGAEKLGIEP
                     QKWKDVGAYGVMKARVVTSGDNVELELRLYEVSKGTNPQLTRTYKGKKADLRGFVHAW
                     CNDVVKYYTGEPGFFGSRIAFVAKGRGASRILAMDFDGANPVNVTRNTSINMLPAWSP
                     SGGQIAFTSYMRNNPDLYVVGAGGGRPRKLSNSRGMNTGASWSPDGSQLAVTLSKDGN
                     P"
BASE COUNT          432 a          833 c          789 g          340 t
ORIGIN      
        1 tctggtcgag ttccagctcg acgccacgcg cggctcgggc ggcctctcgg gcgaagacct
       61 ggtcgaggcc ggacgccggg cgatcgagcg cgagacgctg atcacctact ccgacttcaa
      121 gaagggcgtc ggctccctcg ccacgatcgc caccacggcg ccgttcatcg gcctgttcgg
      181 cacggtggtc ggcatcatgt tcgcgttcca ggagatcagc ggcgccggca acgcgaccct
      241 ccaggtcgtc ggcccgcaca tcgccgaggc gctgttcgcc acggcgatcg gcctggtcgc
      301 ggcggtcccg gcggtgatgg cttacaacta cttcacgcgc cggatccgtg tacagcgctc
      361 ggagatggag accttcgagc aggactatct gaacatcatc aagcgccact tcctgcacta
      421 ggaggccgcc atgggcatgc gcacgggcgg aggcgacgag ctcaacagcg agatcaatgt
      481 cacgccgatg gtcgacgtga tgctggtcct gctcatcatc ttcatgatca cggcgccgat
      541 gatgaacacc ggcgtcgacc tcgacctgcc ccaggtgaac gcccagaaca tcgaggatcc
      601 ggctggtaag ctggtcctct cgatcgatcc ggcccgcaag ctgtacctcg gcggcaccca
      661 ggtcacctgg aaggacctcg aggtgaagct ttccagcaac gagcgcgtca agaaggagca
      721 gcagctgtac atcgaggccg accagagcct gccgtacggc gtcgtcgtca ccgcgatggc
      781 gctggccaag aaggccgggg tggccaaggt gatgatgctg accgatccca ccgagcagat
      841 gaaggtcgaa gagctcgatg tgccgcgctg aacgttcgtg acctccatgc gcgaccccct
      901 ccacctcgcc gccagcctcg gcaccgccgc gaccacggtc gccctgatcg ggctggccgt
      961 cttcgtcgac tgggcgccgg ccgcgctcgc cggcgagggg cccaacctgg acgagatgga
     1021 ggcgatcgag gcgtcgatcg cgtacaagaa ggccgagccg ccgaagcagc cgcagaagca
     1081 gaagcgggcg ccggtgccgg aggagatcga aggggtgtcg cgcgacgaga acaagactcc
     1141 cgtcgagccg aaaaaagacg acaagcgtaa gccggacgag aacttcgagg atcagtacaa
     1201 gaagttccag cgcaaggacg acgaggacct cgaggccggc aagccgaccg acgagaacgt
     1261 gggctcgttc gacggctcgg agttcggctt cgccgaggag accaagggtg acccgtactt
     1321 ccagaagctg gtcctcgacc tccgcgaggg ctgggagtac cccgagatcc tctccgacgt
     1381 cggcgtgccg gtcgcgtgca tccggctggc cgccgatggc aagatcctcg acaccaactt
     1441 ccgcgagcgc agcaacaacg ccgagctcga cgactcggtc gagcgcgcgc tggcggcgct
     1501 gaagaagaag cgcggccagg agccggtgcc ggtgccgacc cacctcctca agtggacgac
     1561 caaatggacc tgctttcgac tcaagccctc cgccgcgcgc tagccgcgct cgccgtcctc
     1621 ggcctcgctg ccgtcgccga cgcccccgcg cgcgccgacg acccgagcgg cgtcatcatc
     1681 gacgtctccg gtgcccgccg cggcctctac ccgctggcga tcccggtcgc ggtcaactcc
     1741 gacggcgccg ccgcccgcga ggtcgccgac atcgcgtcgt tcgacatgca ggtggccggc
     1801 ttcttcaagg tgctcgatcc ggcctcgttc ctggccgacc tgggcgccga gaagctcggc
     1861 atcgagcccc agaagtggaa ggacgtcggc gcctacggcg tcatgaaggc gcgcgtcgtc
     1921 acctcgggcg acaacgtcga gctcgagctc cggctgtacg aggtgtcgaa ggggaccaac
     1981 ccgcagctga cccgcaccta caagggcaag aaggccgacc tgcgcggctt cgtccacgcc
     2041 tggtgcaacg acgtcgtgaa gtactacacc ggcgagcccg gcttcttcgg ctcgcgcatc
     2101 gcgttcgtcg ccaagggccg cggcgcgagc cggatcctgg ccatggactt cgatggcgcc
     2161 aacccggtca acgtcacccg caacacgtcg atcaacatgc tgccggcgtg gtcgccgtcg
     2221 ggcgggcaga tcgccttcac ctcgtacatg cgcaacaacc ccgacctgta cgtggtcggc
     2281 gccggcggcg gccggccgcg caagctgagc aactcgcgcg gcatgaacac cggcgcgtcg
     2341 tggtcgcccg acggcagcca gctggcggtg accctgtcga aggacggcaa cccg
//
LOCUS       DASRIG010000171         5434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3744543, whole genome shotgun sequence.
ACCESSION   DASRIG010000171 DASRIG010000000
VERSION     DASRIG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744543"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFB52_04660"
     CDS             complement(<1..268)
                     /locus_tag="VFB52_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO05656.1"
                     /translation="MSEENYELVLYEVADGVATVTLNNPDKRNMLSGQMLAELVDAMK
                     RAKASDEVRAVVLTGAGDKAFCAGADLGGFAADVPLVHKHFSSDL"
     gene            361..1572
                     /locus_tag="VFB52_04665"
     CDS             361..1572
                     /locus_tag="VFB52_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05657.1"
                     /translation="MESATSSNVIKPDFDSGRKHFIFSQDHEDLRESMRSWVEKELWP
                     HRNEWEDTYWPTSAMERAGELGYLGLCFPEEYGGQGGDYFYSLVRAECMSYSGSGGTN
                     MGFAVQSDMVLPPIHLLGTEEQKRKYLEPGLKGEKVGSLGITEPGAGSDVAGIRTTAI
                     RDGDEYVINGSKTFITNGPRADFIVLVCKTDPDERHAGITLFVVDLRDENGEKVPGFT
                     VSSELEKMGMHASDTGELAFEDVRVPADAVLGEVGKGFYHIAWELQGERLVAAAGCHA
                     GAERMIEKTLEYAKEREAFGRPIGKFQAIRHKFAEMATKTEAAKQFTYSTAWRFGNGE
                     YPVREITMAKLYASRIACEVADDCVQILGGYGYMKEYEIERAYRDVRLNRIGAGTDEV
                     MLDVIGRSYGL"
     gene            1614..2792
                     /locus_tag="VFB52_04670"
     CDS             1614..2792
                     /locus_tag="VFB52_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05658.1"
                     /translation="MTVVKDSGARAVLPPFGEQHEELRDSVARFVAKEIAPHVDEWEA
                     AREFPRELYSRCAEIGFLGLKFPEEYGGQGGDHLHDAIWVEELARSGGSGGVAAGLNA
                     HASIAMPPIFNFGTEEQKQRWLVPGIAGAKIGALGITEPGAGSDVAGISTTAKRDGDR
                     YIVNGSKTFITNGVRADFLVCACKTNEEGGHGGISFLVLEREMPGYEVAAKLEKMGWH
                     SSDTGELAFSDVEVPAENLLGEENGGFKLIMANFAWERLLMAIGAVGAMQRLIEVTVS
                     YALDREAFGRPIGRFQAIRHQVAEMATQAEASRALTYNALRLFHTGQDCIQEVSMAKL
                     VTQRAVLEIADQSLQIHGGYGYMREYGIERAVRDARLGPIGGGTDEIMKEIVGKTMGL
                     "
     gene            complement(2844..3233)
                     /locus_tag="VFB52_04675"
     CDS             complement(2844..3233)
                     /locus_tag="VFB52_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO05659.1"
                     /translation="MADDQIIERFLAAFSSADTESMRALLAPDLVAWVTNADGAMDRV
                     EGRDDYLARTDAMDLPAARFRVELTQPPVPVAPDLVLAMVEVHAERSGRTLHNFAAHL
                     LRLGSDQITEWWMADAKPAESDAFWAS"
     gene            complement(3243..3659)
                     /locus_tag="VFB52_04680"
     CDS             complement(3243..3659)
                     /locus_tag="VFB52_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HZO05660.1"
                     /translation="MTSVLKGFRDFAFKGDLITAAIGLVMALATFELIQAIVEGLLTP
                     LIALIVGDENFFELTFTIGDTSFFYGAVISAAITFVGTAAAVYFLVVTPYKAYQDRRG
                     APVETRPCPECTSSISVAAKRCPNCTSSVAPPAPTA"
     gene            complement(3703..3993)
                     /locus_tag="VFB52_04685"
     CDS             complement(3703..3993)
                     /locus_tag="VFB52_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05661.1"
                     /translation="MISTGMCRCTCGPPITLEMDMAEEIDLNTRDLNLGEMADLFRLI
                     TDTEILVPASRMRETFTLTREKTTIGNALEEFGLVATGNKPEGDGGYADAAA"
     gene            complement(4130..4834)
                     /locus_tag="VFB52_04690"
     CDS             complement(4130..4834)
                     /locus_tag="VFB52_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05662.1"
                     /translation="MPTFRGRRFLVGALAVLALLLAPTAATTADHIVVYGAESGSHLT
                     LSTKGNKIIVKGFQSGRHPHGCWLAPNRMKSVCPARGNAIQVVMGPSGDFVEVADRMP
                     FPLTVYLGAGSDKFIGNAERDTCYSQGARRNRCVGGAGNDVCITGMRNSDCVGNAGDD
                     YCRHQFGSDGCWGGPGNDVCVMGPGQDGCHGEGGNDRLYGGRSPDQLYGGPGWDYCDG
                     QQGWGKSHTCDAGPRR"
     gene            4917..5369
                     /locus_tag="VFB52_04695"
     CDS             4917..5369
                     /locus_tag="VFB52_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZO05663.1"
                     /translation="MIYLLRHGDAEPGNGDDDARRLTAKGERQAADAGSALAALGAEI
                     DACLTSPKLRAAETARLACAALGIEPEVAAELRGGGFDSPTLCAGRGDVLLVGHEPDF
                     SNEVARLTGAKVKLRKGGLAIIDGLTLQALLRPADLRRIAADRAPNPY"
BASE COUNT          925 a         1770 c         1902 g          837 t
ORIGIN      
        1 aaagatcact ggaaaaatgt ttatggacta gggggacatc ggcagcaaag cccccaaggt
       61 cggcccccgc gcagaaggct ttgtcccccg ccccggtgag gacgacggcg cggacctcgt
      121 cgcttgcctt ggcccgcttc atcgcgtcga ccagctcggc gagcatctgc ccgctcagca
      181 tgttgcgttt gtcggggttg ttgagggtga ccgtggcgac gccgtcggcg acctcgtaga
      241 gcaccagctc gtagttctcc tcgctcatcg gcgcggaacc ataacgcaac aagttgaaag
      301 tgggaagtgc tactgtctac tccgctttgg gacctgaacc atctagggag gaatagacca
      361 gtggagagcg cgaccagctc gaacgtgatc aagcccgatt tcgactcggg ccgcaagcac
      421 ttcatcttca gccaggacca cgaagacctg cgcgagtcga tgcgctcgtg ggtcgaaaag
      481 gagctctggc cccaccgcaa cgagtgggag gacacctact ggccgacctc cgcgatggaa
      541 cgagccggtg agctcggcta cctcggcctc tgcttccccg aggagtacgg cggccagggc
      601 ggcgactact tctactcgct ggtccgcgcc gagtgcatga gctactcggg ctccggcggc
      661 accaacatgg gcttcgcggt gcagtccgac atggtcctgc ccccgatcca cctgctcggc
      721 accgaggagc agaagcgcaa gtacctcgaa ccgggcctga agggcgaaaa ggtcggcagc
      781 ctcgggatca ccgagccggg cgccggctcc gacgtcgccg ggatccgcac gaccgcgatc
      841 cgcgacggtg acgaatacgt gatcaacggc tcgaagacgt tcatcaccaa cggcccgcgg
      901 gccgacttca tcgtgctggt ctgcaagacc gacccggacg agcgccacgc cggcatcacc
      961 ctgttcgtcg tcgacctgcg cgacgagaac ggcgagaagg tccccggctt caccgtttcc
     1021 agcgagctgg agaagatggg gatgcacgcc tccgacaccg gcgaactcgc cttcgaagac
     1081 gtccgcgtcc cggccgacgc cgtgctcggc gaggtcggca agggcttcta ccacatcgcc
     1141 tgggagcttc agggcgagcg gctggtcgcc gccgccggct gccacgcggg agccgagcgg
     1201 atgatcgaga agaccctcga gtacgcgaag gagcgcgaag ccttcggccg gccgatcggc
     1261 aagttccagg cgatccgcca caagttcgcc gagatggcga ccaagaccga agcggccaag
     1321 cagttcacct actcgaccgc ctggcgcttt ggcaacggcg agtacccggt gcgcgagatc
     1381 acgatggcga agctctacgc atcgcggatc gcctgcgagg tcgccgacga ctgcgtccag
     1441 atcctcggcg gctacggcta catgaaggag tacgagatcg agcgcgccta ccgcgacgtg
     1501 cggctcaacc gaatcggtgc cggcaccgac gaggtgatgc tcgacgtgat cgggcgcagc
     1561 tacgggctct aggtcaattt gttcggagga gccgaacgaa ttgacacggt gaagtgaccg
     1621 tcgttaagga cagtggggcg cgggcggtgc tgccgccgtt tggcgagcag cacgaggagc
     1681 tgcgcgattc ggtcgcgcgg ttcgtcgcca aggagatcgc gccccacgtc gacgagtggg
     1741 aggccgcgcg ggaattcccg cgggagctct actcgcgctg cgctgagatc ggtttcctcg
     1801 ggctgaagtt cccggaggag tacggcggcc agggcggcga ccacctgcac gacgcgatct
     1861 gggtcgagga gctggcccgt tcgggcgggt ccggcggcgt cgccgccggg ctcaatgccc
     1921 acgcctcgat cgcgatgccg ccgatcttca acttcggcac cgaggagcag aagcagcgct
     1981 ggctggtgcc ggggatcgcc ggggcgaaga tcggcgcgct ggggatcacc gagccggggg
     2041 cgggctccga cgtcgccggc atctccacca ccgccaagcg tgacggcgac cgctacatcg
     2101 tcaacggctc gaagaccttc atcaccaacg gcgtgcgggc cgacttcctc gtctgcgcct
     2161 gcaagacgaa cgaggagggc ggccacggcg gcatctcctt cctcgtgctc gagcgcgaga
     2221 tgcccggcta cgaggtggcg gcgaagctgg agaagatggg ctggcactcc tcggacacgg
     2281 gagagctcgc cttcagcgac gtcgaggtcc cggccgagaa cctgctcggc gaggagaacg
     2341 gcggcttcaa actgatcatg gccaacttcg cctgggagcg gctattgatg gcgatcggcg
     2401 ccgtcggcgc gatgcagcgt ctgatcgagg tgaccgtctc ctacgccctc gaccgcgaag
     2461 ccttcggccg cccgatcggc aggttccagg cgatccgcca ccaggtcgcc gagatggcga
     2521 cccaggccga ggcctcccgc gccctcacct acaacgccct acgcctcttc cacaccggcc
     2581 aggactgcat ccaagaggtc agcatggcca agctagtcac ccagcgcgcc gtcctcgaga
     2641 tcgccgacca gtccctccag atccacggcg gctacggcta catgcgcgag tacggcatcg
     2701 agcgcgccgt ccgcgacgcc cgcctcggcc cgatcggcgg cggcaccgac gagatcatga
     2761 aggagatcgt cggcaagacg atggggttgt gaccttgtcg agttagtcac gcacgtgggt
     2821 gactaactcg acagggagcc ggatcagctt gcccagaagg cgtcgctctc cgccggtttg
     2881 gcgtcggcca tccaccactc ggtgatctgg tcgctgccga ggcggaggag gtgggcggcg
     2941 aagttgtgga gggtgcggcc ggagcgttcg gcgtggacct cgaccatggc gaggacgagg
     3001 tcgggggcga cggggacggg gggttgggtg agctcgacgc ggaagcgggc ggcggggagg
     3061 tccattgcgt cggtccgggc gaggtagtcg tcgcggccct cgacgcggtc catggcgccg
     3121 tcggcgttgg tcacccaggc gaccaggtcg ggggcgagga gggcgcgcat cgactcggtg
     3181 tcggcgctcg agaaggcggc gaggaagcgc tcgatgatct ggtcgtcggc catgagagcg
     3241 cgctaggcgg tcggcgccgg cggggcgacc gatgaggtgc agttgggaca gcgcttggcc
     3301 gcgaccgaga tcgaggaggt gcactcgggg caggggcggg tctcgaccgg ggcgccgcgg
     3361 cggtcctggt aggccttgta gggagtgacg acgaggaagt agacggcggc ggccgtgccg
     3421 acgaaggtga tcgcggcgct gatcacggcg ccgtagaaga agctggtgtc gccgatcgtg
     3481 aaggtcagtt cgaagaagtt ctcatcgcca acgatcaggg cgatcagcgg ggtgagcaga
     3541 ccttcgacga tcgcctggat cagctcgaaa gtggccaggg ccatgaccag gccgatcgcg
     3601 gccgtgatca ggtcgccttt gaaggcgaag tccctgaacc ccttgagcac cgaggtcacg
     3661 cgacgttatt cgccgcgcat agcgggcctc ctggcccaag cctcaggcag cggcgtccgc
     3721 gtagccccca tcgccctcgg gcttgttgcc ggtggcgacg aggccgaact cctcgagggc
     3781 gttgccgatc gtcgtcttct cccgggtcag cgtgaaggtc tcgcgcatcc gggaagcggg
     3841 gacgaggatc tctgtgtcgg tgatcagccg gaacaggtcg gccatttcac cgaggttgag
     3901 gtcacgggtg ttgaggtcga tttcctccgc catgtccatt tcgagcgtga tcggcggtcc
     3961 gcatgtacag cggcacatgc cggtgctgat cacatgcaca cagccgcagc tgccagcttc
     4021 cgggcaaacg gagtcgcagt cacaagccat cggggtacct ccctggtcga gaacggtcgt
     4081 tccgcggacc ctagtcccaa tggacgaatg ttgctgaata ttctggtttt tatcttcgcg
     4141 ggccggcgtc gcaggtgtgg gacttgcccc atccctgctg gccgtcgcag tagtcccaac
     4201 cgggaccgcc gtagagctgg tcgggggagc ggccgccgta gaggcggtcg ttgccgcctt
     4261 cgccgtggca gccgtcctga ccggggccca tcacgcagac gtcgttgccg gggccgcccc
     4321 aacagccgtc gctgccgaac tggtggcggc agtaatcgtc gccggcgttg ccgacgcagt
     4381 cgctgttgcg catgccggtg atgcagacgt cgttgccggc accgccgacg cagcgattgc
     4441 gcctggcgcc ctgcgagtaa caggtgtcgc gctcggcgtt gccgatgaac ttgtccgagc
     4501 cggccccgag gtagaccgtg agcgggaagg gcatgcggtc ggcgacctcg acgaagtcgc
     4561 ccgaggggcc catcacgacc tggatcgcgt tgccgcgggc agggcagacc gacttcatcc
     4621 ggttcggcgc cagccagcag ccgtgcgggt gtctgccgga ctggaagccc ttgacgatga
     4681 tcttgttgcc cttcgtggac agggtcaggt gggagccgga ctcggctccg tagaccacga
     4741 tgtggtcagc cgtggtcgcc gcggtcgggg ccaagagaag ggccaggacc gcgagcgcac
     4801 ctacgagaaa acgtcggcct cggaaggtcg gcattccggc caactgtaac caggtcgccg
     4861 gttgggcaaa catccccttg cgtacacggg gggatttcta tgcctacgat caacggatga
     4921 tttacctgct ccggcacggg gacgccgagc ccggaaacgg cgacgacgat gcccgtcggc
     4981 tgaccgccaa gggcgagcgc caggcggccg acgcgggcag cgcgctcgcc gccctgggcg
     5041 ccgagatcga cgcctgcctg accagcccca agctgcgtgc cgccgagacc gcgcggctcg
     5101 cctgcgcggc cctcgggatc gagcccgagg tcgcggccga gctgcgcggc ggcggcttcg
     5161 actcccccac gctctgcgcc gggcgcggcg acgtgctcct ggtcgggcac gagcccgact
     5221 tctccaacga ggtcgcccgc ctcaccggcg ccaaggtgaa gctgcgcaag ggcggcctcg
     5281 cgatcatcga cggcctgacc ctgcaggcgc tgctgcgccc cgccgacctg cgccgcatcg
     5341 ccgctgaccg cgccccgaat ccctactagc cttcccccac caccaacgac cggagggggc
     5401 accaatgctg tcgtggctgg ccaagaagat gatc
//
LOCUS       DASRIG010000172         5575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1138658, whole genome shotgun sequence.
ACCESSION   DASRIG010000172 DASRIG010000000
VERSION     DASRIG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138658"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFB52_04700"
     CDS             complement(<1..650)
                     /locus_tag="VFB52_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZO05664.1"
                     /translation="MAGRAAAEALVARAGGSAVRVWDEAADPPQRARADGLRALGVEC
                     LLGDDGRAALAGARTVVKSPGVPWDAPPVAEALRRGVPVVDELEIGWRLVPAPTVAVT
                     GTNGKTTVAGLLVSVLAAHGLAPALTGNTAFGPPLSALSLGPPPRSLVAEVSSYQAEA
                     SPELAADAAIFTNLSNDHLNRHGTMAAYAYAKRALFVKGEWAVPVAALNVDDELGR"
     gene            822..2102
                     /locus_tag="VFB52_04705"
     CDS             822..2102
                     /locus_tag="VFB52_04705"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZO05665.1"
                     /translation="MADVRPFKALHYDLGKVGGLDAVAAPPYDVIDAPERARLLAQSP
                     YNAVAIDLPKPFDPADPDSNPDYDPYEKAARTIDAWREEGVLVDDSEPAFWALTQDYT
                     APDGSQHSRHGILAQVRVEDYETGTVRPHERTHPGPLLDRLELTRATHLNLSPIFSLS
                     TADPWPLVEPALGEPWGEATDPDGTVNRGWRVADPAVLAAVTELLADAQLLIADGHHR
                     YETARAYRDEVGGEGDHNYTLMSLTGLDDPGLTVFPTHRLLSGFAANPERMAAFGEGM
                     RGLFESEAVERDAIDPGGEDGVGVYGLYDSFHKQAYRLRLKDVEALDRQLEGKPEAYR
                     RLDSAILETLVLKGLAGMSDHDIDERNGLEYAKSVPDALARVDAGDFDIALIQRPVPV
                     EQVKAVAETDENMPPKSTYFFPKVMTGMVFSPVG"
     gene            2138..2701
                     /locus_tag="VFB52_04710"
     CDS             2138..2701
                     /locus_tag="VFB52_04710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZO05666.1"
                     /translation="MVKIYTKKGDDGSTSLWYGGRVPKHHGRTEAYGALDEACSQLGA
                     ARALCDEDQAELAADILQLQNDIFIAGAELATAPEAAERLEDGISRVTEEMVAEHERL
                     IDRYMSEVELPPKFVIPGGNQLSAQLDIARTVIRRAERWISALNEEGEIASETVIHFV
                     NRASDLAYAMARWADVPDPALFEGRGK"
     gene            2705..3097
                     /locus_tag="VFB52_04715"
     CDS             2705..3097
                     /locus_tag="VFB52_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO05667.1"
                     /translation="MKVAARRIEGFAHEIELEGGHELVVDEPEDRGGTDTGPRPTQLL
                     ASSLAGCIAITMEMYADRKGWNLDELEVSVEVTYEGAVPNDYEVSISLPDGLDEDQRR
                     RLLVIATKCPVHKVLAGEASVRVHEELA"
     gene            3097..3909
                     /locus_tag="VFB52_04720"
     CDS             3097..3909
                     /locus_tag="VFB52_04720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05668.1"
                     /translation="MDLGLAGKACAVTGASRGIGRETALTLCAEGAHVLLIARDEHRL
                     REVQEEADGAGRTAGGRAAILALDVTEEAAGERTLATATEYFGQLDVLVNNAGFASWR
                     DLEDVPDEDWRAQYEINVMAPLRAMRAAAPAMAERGWGRIVNVCSTAGKRPSSAMPEY
                     SVAKAAELSLSRLFADRYAKHGVLVNAITPGPTESSMWMAPGGLADQSRELAGAESRE
                     QAISEAGAKRPIGRLATSDEIASAIVFLCSERASYVAGAAWSVDGGTVPVII"
     gene            3906..4481
                     /locus_tag="VFB52_04725"
     CDS             3906..4481
                     /locus_tag="VFB52_04725"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZO05669.1"
                     /translation="MTAELAALLLDPAEVAASAGPGVEAAVLVPIYGWPDEPGMIFTE
                     RRADLRRHAGEISFPGGRRDPGDADLAATALRETAEEIALDPAVVELRGALPAISTFV
                     TNYRVHPFVGLVPDPLELNLQPNPGEVETVLTVSLQRLREGYEMRRLVRRGVPIRTPT
                     YELDGGQLIWGATARILGEFIDRLDAADAPA"
     gene            complement(4478..5326)
                     /locus_tag="VFB52_04730"
     CDS             complement(4478..5326)
                     /locus_tag="VFB52_04730"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZO05670.1"
                     /translation="MSYSKDVLVDTEWVAAHLDDDSVRIVEVDENPALYAEGHIPGAI
                     GLDWKADLQDPVERNFLGPEAFAALIGGRGISNDHTVVLYGDRNNWFAAYTYWYLRYY
                     GHEDVKLVDGPREKWVAEGRPTSTEVPSYEAERFAVTAGDDTIRAYRWDVEAALGNGT
                     NLVDVRSPQEFAGELIAPTGYEQEGAQRGGHIPGAASVPWASAVREDGTFKSAEELRD
                     LYAGKGVLEDEKPVIAYCRIGERSAHTWFVLHELLGTRSVRNYDGSWTEWGNLVGAPI
                     EKGAPV"
     gene            complement(5393..>5575)
                     /locus_tag="VFB52_04735"
     CDS             complement(5393..>5575)
                     /locus_tag="VFB52_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05671.1"
                     /translation="VYEGVGSAPAGLLADGAEIVRTAGRTWRIPVVVAEADPEDPETW
                     AEEMRKAALGAISGAG"
BASE COUNT          829 a         2121 c         1877 g          748 t
ORIGIN      
        1 tcccgaccca actcgtcgtc gacgttgagc gccgccaccg gcaccgccca ctcacccttg
       61 acgaagagcg cccgcttggc gtaggcatag gcggccatcg tcccgtgccg gttcaggtgg
      121 tcgttgctga ggttggtgaa gatcgccgcg tcggcggcca gctcggggct cgcctcggcc
      181 tgatagctgg agacctcggc gaccagcgag cgcggcggcg ggccgagcga aagcgcgctg
      241 agcggcgggc cgaaggcggt gttgccggtc agcgccggcg ccaggccgtg cgcggcgagt
      301 accgagacca gcagcccggc aaccgtcgtc ttgccgttgg tgccggtgac ggcgacggtc
      361 ggtgcgggca ccagccgcca accgatctcg agctcgtcga ccaccggcac gccccgccgc
      421 agcgcctcgg cgaccggcgg cgcgtcccag ggcacgccgg gactcttgac cacggtgcgg
      481 gcgccggcga gcgccgctcg cccgtcgtcg ccgagcaggc actcgacgcc gagcgcgcgc
      541 agcccgtcgg cccgtgcccg ctgcggcgga tcggcggcct cgtcccagac ccggaccgcg
      601 ctgccaccgg cccgagcaac caacgcctcg gccgccgccc gcccggccac gccgagcccg
      661 gcgacgagaa agggcgcggg tggcagcgct gggcgccgtc tcgtggcccc gcggaagaac
      721 gcaggcatct cggctgcgcg tctctcctcg ttctcccgca gcatcgtcgc cactacaggc
      781 cgcctaccca ctcgcggcgt ttctagttag cgttaggccg aatggccgat gtccgaccct
      841 tcaaggcact gcactacgac ctcggcaagg tcggcggcct cgacgccgtc gccgccccgc
      901 cctacgacgt gatcgacgct cccgagcgcg cccgcctgct cgcccagtcc ccctacaacg
      961 cggtcgcgat cgacctgccg aagccgttcg acccggcgga tcccgacagc aacccggact
     1021 acgaccccta cgaaaaagcc gcgcgcacga tcgacgcctg gcgcgaggag ggcgtgctcg
     1081 tcgacgactc cgagccggcc ttctgggcgc tgacccagga ctacaccgcg cccgacgggt
     1141 cgcagcacag ccgccacggg atcctcgccc aggtccgagt cgaggactac gagaccggca
     1201 ccgtgcgccc gcacgagcgc acccaccccg gcccgctgct cgaccgcctc gagctgaccc
     1261 gcgccaccca cttgaacctc tcgccgatct tctcgctctc caccgccgac ccgtggccgc
     1321 tggtcgagcc ggcgctgggc gagccctggg gcgaagcgac cgatcccgat gggaccgtga
     1381 accggggctg gcgggtcgcc gacccggcgg tgctggccgc ggtcaccgag ctgctcgccg
     1441 acgcgcagct cctgatcgcc gacggccacc accgctatga gaccgcgcgc gcctaccgcg
     1501 acgaggtcgg cggcgagggc gaccacaact acacgctgat gtcgctgacc gggctcgacg
     1561 atcccggcct caccgtcttc cccacccacc gcctgctctc cggcttcgcc gccaacccgg
     1621 agcggatggc cgccttcggc gaggggatgc gcgggctgtt cgagagcgaa gcggtcgagc
     1681 gcgacgcgat cgatccgggc ggcgaggacg gcgtcggcgt ttacggcctc tacgactcct
     1741 tccacaaaca ggcctaccgg ctgcgcctca aggacgtcga ggcactcgac cgccagctcg
     1801 agggcaaacc ggaggcctac cgccggctcg actcggcgat cctcgagacc ctcgtgctca
     1861 agggcctggc ggggatgagc gaccacgaca tcgacgagcg caacggcctc gagtacgcca
     1921 agagcgtgcc cgacgcgctc gcccgggtcg acgccggcga cttcgacatc gccctgatcc
     1981 agcgcccggt gccggtcgag caggtcaagg cggtcgccga aaccgacgag aacatgccgc
     2041 ccaagtccac ctacttcttc cccaaggtga tgaccggcat ggtcttcagc ccggtcggct
     2101 gagcggggcc ggcaggcgcg cggtctagga tcaggccgtg gtcaagatct acacgaagaa
     2161 gggtgacgac ggctcgacct cgctctggta cggcggtcgc gtccccaagc accacggccg
     2221 caccgaggcc tacggcgccc tcgacgaggc ctgctcgcag ctcggcgccg cccgcgctct
     2281 ctgcgacgag gaccaggccg agctggccgc cgacatcctg cagctgcaga acgacatctt
     2341 catcgccggc gccgagctgg cgaccgcacc ggaggcggcg gagcgactcg aagacgggat
     2401 cagccgcgtc accgaggaga tggtcgccga gcacgagcgg ctgatcgacc gctacatgtc
     2461 cgaggtcgaa ctgccgccga agttcgtgat ccccggcggc aaccagctct ccgcccagct
     2521 cgacatcgcc cgcaccgtga tccgccgcgc cgagcgctgg atctccgccc tcaacgagga
     2581 aggcgagatc gccagcgaga ccgtgatcca cttcgtcaac cgcgcctccg acctcgccta
     2641 cgcgatggcc cgctgggccg acgtccccga ccccgccctg ttcgagggcc gcggcaagta
     2701 acgcgtgaag gtcgccgccc ggcgcatcga gggcttcgcc cacgagatcg agctcgaggg
     2761 tggccacgag ctggtcgtcg acgagcccga ggaccgcggc ggcaccgaca ccggcccgcg
     2821 cccgacccag ctcctggcct ccagcctcgc cggttgcatc gcgatcacca tggagatgta
     2881 cgccgaccgc aaaggctgga atctggacga gctcgaagtc agcgtcgagg tcacctacga
     2941 gggcgcggtc ccgaacgact acgaggtctc gatctcgctc cccgacgggc tcgacgagga
     3001 ccagcgccgc cgcctgctgg tgatcgcgac caagtgcccg gtgcacaagg tgctggccgg
     3061 cgaggcctcg gtgcgcgtgc acgaggagct cgcctgatgg acctcgggct cgccggcaaa
     3121 gcctgcgcgg tgaccggcgc cagccgcggg atcggccgcg agacggcgct gacgctctgc
     3181 gccgagggcg cccatgtgct gctgatcgcg cgcgacgagc accgcctgcg cgaggtccag
     3241 gaagaggccg acggcgccgg ccgcacggcc ggcgggcgcg ccgcgatcct cgccctcgac
     3301 gtcaccgaag aggcggccgg cgagcgcacc ctcgcgaccg cgaccgagta cttcggccag
     3361 ctcgacgtgc tcgtcaacaa cgccgggttc gccagttggc gcgacctcga ggacgtcccc
     3421 gacgaggact ggcgcgccca gtacgagatc aacgtgatgg cgccgctgcg ggcgatgcgc
     3481 gccgccgcgc cggcgatggc cgagcgcggc tggggccgga tcgtcaacgt ctgctcgacc
     3541 gccggcaagc gcccctcctc ggcgatgccg gagtactcgg tggcgaaggc ggccgagctc
     3601 tcgctcagcc gcctgttcgc cgatcgctac gcaaaacacg gcgtgctggt caacgcgatc
     3661 acgccggggc cgaccgaatc gtcgatgtgg atggcgcccg gcggcctcgc cgaccagtca
     3721 cgcgagctgg ccggcgccga gagccgtgag caggcgatct ccgaagccgg cgccaagcgc
     3781 ccgatcggcc gcctcgccac cagcgacgag atcgcctcgg cgatcgtctt cctctgctcc
     3841 gagcgcgcct cctacgtcgc cggcgccgcc tggtctgtcg acggcggcac ggtgccagtg
     3901 atcatttgac cgccgagctc gcggcgctgc tgctcgaccc cgccgaggtg gcggcgagcg
     3961 cggggccagg ggtcgaagcg gcggtcctgg tgccgatcta cggctggccc gacgagccgg
     4021 gcatgatctt caccgagcgc cgcgccgacc tgcgccgcca cgccggcgag atctccttcc
     4081 ccggcgggcg ccgcgacccc ggcgacgccg acctggcggc gaccgcgctg cgcgagaccg
     4141 cggaggagat cgccctcgac cccgccgtgg tcgagctgcg cggcgccctg ccggcgatct
     4201 ccaccttcgt caccaactac cgcgtccacc ccttcgtcgg cctcgtcccc gacccgctcg
     4261 agctcaacct gcagccgaac ccgggcgagg tcgagaccgt gctcacggtc tcgctccagc
     4321 gcctgcgcga gggctacgag atgcgccgcc tcgtccgccg cggcgtcccg atccgcaccc
     4381 ccacctacga gctcgacggc ggccagctga tctggggcgc caccgcgcgg atcctcggcg
     4441 agttcatcga ccgcctcgac gctgccgacg cgccggccta aaccggcgcg cctttctcaa
     4501 tcggagcacc gacgaggttg ccccactcgg tccaggagcc gtcgtagttg cggacgctcc
     4561 gcgtcccgag cagctcgtgc aggacgaacc aggtatgggc ggaacgctca ccgatccggc
     4621 agtaggcaat caccggcttc tcgtcctcga gtacgccctt cccggcgtag aggtcgcgca
     4681 gctcctcggc cgatttgaag gtgccgtcct ccctgaccgc gctggcccag gggaccgagg
     4741 cggcgccggg gatgtggccg ccgcgctggg cgccctcctg ctcgtagccg gtcggcgcga
     4801 tcagctcgcc ggcgaactcc tgcgggctgc gcacgtcaac cagattcgtg ccgttgccga
     4861 gcgccgcctc gacgtcccag cggtaggcgc ggatcgtgtc gtcgccggcc gtgaccgcga
     4921 accgctcggc ctcgtaggac ggcacctcgg tcgaggtcgg ccgcccctcc gcgacccact
     4981 tttcccgcgg gccgtcgacc agcttcacgt cctcgtggcc gtagtagcgg aggtaccagt
     5041 aggtgtaggc ggcgaaccag ttgttgcgat cgccgtagag gacgacggtg tggtcgttgg
     5101 agatgccgcg cccgccgatc agcgcggcga atgcctccgg gccgaggaag ttgcgctcga
     5161 ccgggtcctg caggtcagcc ttccagtcga gcccgatcgc gccggggatg tggccctcgg
     5221 cgtagagggc cgggttctcg tcgacctcga cgatgcggac cgagtcgtcg tcgaggtggg
     5281 cggcaaccca ctcggtgtcg acgaggacgt ccttcgagta gctcatttga acctccaatg
     5341 ggagaactcc gtatgaaata cctaaattcc catcggattt tagctctttg agttagccgg
     5401 ctccggaaat ggctcccaga gcggctttgc gcatctcctc agcccacgtc tcggggtcct
     5461 cgggatccgc ctcggcgacc acgaccggga tccgccaggt gcgccccgcg gttcgcacga
     5521 tctccgcccc gtcggccagc agcccggcag gggcgctccc caccccctcg tagac
//
LOCUS       DASRIG010000173         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1649167, whole genome shotgun sequence.
ACCESSION   DASRIG010000173 DASRIG010000000
VERSION     DASRIG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649167"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="uvrA"
                     /locus_tag="VFB52_04740"
     CDS             complement(<1..1619)
                     /gene="uvrA"
                     /locus_tag="VFB52_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZO05672.1"
                     /translation="MPASDRIVISGAREHNLKDVDVELPRDALIVITGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGIPHCPECGEQITGQTQEQIVDRLMTLPEGTKFMVMAPVVRG
                     RKGEYGKLMEQMRLEGYSRVKVNGELRRLDEEIVLDKKFKHDISVVVDRLVMKTDLRR
                     RLSESVEAAGGLAAGLIEVEIVEGEEAGETLLFSEQFACLNCGTSIPELEPRIFSFNS
                     PHGACERCHGLGFQRVIDPDLVVPDPTLSLAEGALQPWNRGITAYWRRLIATVAEEYG
                     VDADKPWSKLKKDEKDLFLYGTGEDRHQVSYTNRFGRRRSYKVRFEGIVNNLQRRYEE
                     TDSENSRERIEGYMAEQPCPVCHGARLRAESLAVKVGGFSIAEYSDLSARAAAEWINA
                     LELTDTERAIARLIVREITERLAFLDNVGIGYLSLARSARSLSGGEAQRIRLATQIGS
                     SLVGVMYVLDEPSIGLHQRDNEKLIATLDRLR"
     gene            complement(1676..>2036)
                     /locus_tag="VFB52_04745"
     CDS             complement(1676..>2036)
                     /locus_tag="VFB52_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05673.1"
                     /translation="GLSMDYHVFILTRVREAYDHGMTTEQAVSSAIKKTAGVVTSAAV
                     VMVAVFAIFASLSMIEFKQMGVGLAVAILLDATLVRAVLLPAAMKLLGERNWYLPRAL
                     GWLPKVSHEPAPTPEAA"
BASE COUNT          325 a          652 c          720 g          339 t
ORIGIN      
        1 cggaggcggt cgagggtcgc gatcagcttc tcgttgtcgc gctggtggag gccgatcgag
       61 ggctcgtcga gcacgtacat caccccgacc aggctggagc cgatctgggt ggcgagccgg
      121 atccgctgcg cctcgccgcc ggagagcgag cgggccgagc gggccagcga gaggtagccg
      181 atcccgacgt tgtcgaggaa ggcgagccgt tcggtgatct cgcggacgat cagccgggcg
      241 atcgcgcgct cggtgtcggt cagctcgagc gcgttgatcc actcggcggc ggcgcgggcc
      301 gagaggtcgc tgtactcggc gatgctgaag ccaccgacct tgaccgcgag gctctcggcg
      361 cgcaggcggg cgccgtggca gaccgggcag ggctgctcgg ccatgtagcc ctcgatccgc
      421 tcgcgcgagt tctcggagtc ggtctcttcg tagcggcgct ggaggttgtt gacgatcccc
      481 tcgaagcgca ccttgtagga gcggcggcgg ccgaagcggt tggtgtagga gacctggtgg
      541 cgatcctcgc cggtgccgta gaggaagaga tctttctcgt ccttcttcag cttcgaccag
      601 ggcttgtcgg cgtcgacgcc gtactcctcg gcgacggtcg cgatcaggcg ccgccagtag
      661 gcggtgatcc cgcggttcca gggctgcagg gctccctcgg cgagcgagag ggtcgggtcg
      721 gggacgacca ggtcggggtc gatcacccgc tggaagccga gcccgtggca gcgctcgcag
      781 gcgccgtgcg gcgagttgaa ggagaagatc cgcggctcca gctcggggat cgaggtgccg
      841 cagttgaggc aggcgaactg ctcggagaag agcagcgtct cgccggcttc ctcgccctcg
      901 acgatctcga cttcgatcaa cccggcggca aggccgccgg cggcctcgac cgactcggag
      961 aggcggcggc gcaggtcggt cttcatcacc agccggtcga ccactaccga gatgtcgtgt
     1021 ttgaattttt tgtcgaggac gatctcctcg tcgagccgac gcagctcgcc gttgaccttg
     1081 acccgggagt agccctcgag ccgcatctgc tccatcaatt tcccgtactc gcctttgcgc
     1141 ccgcgcacga ccggggccat gaccatgaat ttggtcccct ccggcaacgt catcagccga
     1201 tcgacgatct gctcctgggt ctggccggtg atctgctcgc cgcactcggg gcagtgcggg
     1261 atcccgatcc gggcccagag cagccgcagg tagtcataaa tctcggttac ggtccccacg
     1321 gtcgagcgcg ggttgcgcga ggtggtcttc tggtcgatcg agatcgccgg cgacagaccc
     1381 tcgatcgagt cgacgtcggg cttctccatc agcccgagga actgccgcgc gtaggcggag
     1441 agcgactcga cataacgccg ctgcccctcc gcgtagatcg tgtcgaaggc aaggctcgac
     1501 ttccccgacc cggagaggcc ggtgatcacg atcagcgcgt cacgggggag ctcaacgtcg
     1561 acatccttga ggttgtgctc acgggcgccg gagatgacga ttcggtctga agcaggcact
     1621 cgcttacgag tctagcgagg cgaacagatg ttcccctccg gggaccccga agccgctaag
     1681 cggcttcggg ggtcggagcg ggctcgtgac tgactttggg gagccagccg agcgcgcggg
     1741 gcaggtacca gttgcgctcg ccgagcagct tcatcgccgc cgggagcagg accgcgcgga
     1801 ccagggtcgc gtccagcagg atcgcgacgg ccaggccgac gcccatctgc ttgaactcga
     1861 tcatcgagag ggaggcgaag atcgcgaaca cggcgaccat cacgaccgcg gcgctcgtga
     1921 cgacgccggc ggtcttcttg atcgccgacg aaacggcctg ctccgtcgtc atgccgtggt
     1981 cataggcctc acgcacgcgc gtgaggatga acacgtggta gtccatcgac aggccg
//
LOCUS       DASRIG010000174         8691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3746222, whole genome shotgun sequence.
ACCESSION   DASRIG010000174 DASRIG010000000
VERSION     DASRIG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746222"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFB52_04750"
     CDS             complement(<1..227)
                     /locus_tag="VFB52_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZO05674.1"
                     /translation="MSTESETTLAPVPFMSLERHHRPIAGELRRAFDDVLADGGFVLG
                     PEVERFEEEFAAYCGVGHCVGVNSGTAALALA"
     gene            459..2432
                     /gene="ftsH"
                     /locus_tag="VFB52_04755"
     CDS             459..2432
                     /gene="ftsH"
                     /locus_tag="VFB52_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZO05675.1"
                     /translation="MSNPRLAKAKKESSFLRGAKERLAPIGRFLVKDKLSTFLLVASI
                     GLLVLFFSLLGSLGPEGEGTKVPLSTISNVGESGRIATAELLDYDHQVIAHTELGLDY
                     YADYPASDAATQQLYDQLSGGGASVSVDPQSGKAERTVVVQFLLPILILVCLFAFFIR
                     QTGDGSGGIGAFSVFGGKGKRRKKGEKASITFDSVAGAGEALAELKEIRDFLDDPTKY
                     LALGAAAPKGVLLVGPPGTGKTLLARATAGEADAAFFSASGAEFVESLVGVGAARIRD
                     LFAKARKMSPAIIFIDELDAAGRKRGAGVGQGNDEREQTLNQLLVEMDGFGGDAGVVV
                     MGATNRPDILDPALLRPGRFDRQVVIDVPDVHGRYEILELHAKGRSFAAGVDLSEIAK
                     LCPGFSGAELANLVNEAALLTVREGRSEIDQPTLEEAIDRVVAGPAKKSHVLSEDERW
                     TIAVHEAGHAVATRSIGQTVSAQKLSIVARGRQLGTAAHMLTDRDAVMHAQSDLERQL
                     VAILSGTAAEWAEFGEGSTGASDDLHAATKLARQMVTSFGMSPRLGSVTIGEAGGEVF
                     LGASLQDMGSVGPHTLDVIDEETERIVEEAKARALFVLRGNWEAVQEIARALLEQETL
                     SGVALEALLSTVAEFELGEIALPGPEVRSDGEA"
     gene            complement(2437..3273)
                     /locus_tag="VFB52_04760"
     CDS             complement(2437..3273)
                     /locus_tag="VFB52_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZO05676.1"
                     /translation="MASPTEIGRRHLGRARRSGEHALRRASAGSRPLPGFLIVGAQKA
                     GTTSLLDLLCQHPGVAAPVRKEVHFFDHEFARGPDWYRAHFPRARAATVSGEATPYYL
                     FHPLAPRRAAELVPDARLIVLLRDPIDRAFSQHNHERVLGFEQLDFEAALAAEAARLG
                     GEEERLIAEPGYRSFAHQHHSYLERGRYAGQLERWLECYPRERVLVLSAEDLFADPVS
                     TAAEAQVFLGLERLAPADTAAKNARSYAPIEPAARARLRAELAADNARLYELVGREFG
                     WD"
     gene            3390..4181
                     /locus_tag="VFB52_04765"
     CDS             3390..4181
                     /locus_tag="VFB52_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05677.1"
                     /translation="MLIAVVIFAGIGATLALVRTPDYTATARLSVGRIDITSPGALSG
                     FAVATQALATGYSRTVTSQAVATEVSEKTGISVKDVQSHVTATPIAESPVFRIEATSP
                     DREQAIALANLSGQALIRYAAKLNRDNPDTARLFAKYRQAAGDATNAEQELEAAEEDL
                     EEREIPPVEKQVDRAEADLEAAELRAEAFGRAYTSSVQSQVATDLVQMISPAIDAESD
                     KRSTFFIYAFIGLVIGLFVGGALAYWREWRLSVGAASRAREAESG"
     gene            complement(4178..5260)
                     /locus_tag="VFB52_04770"
     CDS             complement(4178..5260)
                     /locus_tag="VFB52_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05678.1"
                     /translation="MPRSLAKTPTRKVRAALIAAALACVLLLLLVPPAGADAAGVAAR
                     KGSKAKASADHFRFFSPASFWNAPVPAAAKLDQRSAKLVRALSTEVEAELADASGPWI
                     GTSRYSVPIYTVPASQPAVRVKLTSPIFSPPLQAAFDRVPLPPEATPSGGTDGHLVVW
                     QPSTDRMWEFWRLVPGAEPSASWGGAMENVSANRGVYDTDAWPDAEPWWGASASSLPV
                     AGGLITLEDLREGEIDHALAIALPEIRAGVYAAPAQRTDGKSASPLALPEGARLRLDP
                     KLDLAKLQMPRVTRLIAEAAQRYGIFVRDGAGGVQLFAQDPVTAAKNPYTGPNGYFEG
                     RYPHQLLASFPWERLQVLKLDLHPDG"
     gene            5341..6375
                     /locus_tag="VFB52_04775"
     CDS             5341..6375
                     /locus_tag="VFB52_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05679.1"
                     /translation="MRLLLALSCLAALAGGCGSDALPEGPTGPAPKGEQSTAFRFFSP
                     ASFWNTPVPAGAAIDPGSPRLVRALDTEVAAEQAAARGPWISTTRYSIPIYTVPAEQP
                     TVRVRLTSPVHSAALQAAFEAVPLPPGAYPSGGTDGHLVVWQPKTDRLWEFWRLRRRP
                     DGPSASWGGAIERVSESTGVYDADVWHNAETWWGASASSLSIAGGLITLDDLRHGKID
                     HALALALPQIRAGVYASPAQRTDGKSADPRSLPEGAHLRLDPDLDLDSLPMPPLTRMI
                     AAAAQRYGLFVRDGSAVVQLFAEDPVTAPHNPYTGPTGYFEGRYPNELLASFPWHRLE
                     VLDMDLRRDR"
     gene            complement(6426..>8691)
                     /locus_tag="VFB52_04780"
     CDS             complement(6426..>8691)
                     /locus_tag="VFB52_04780"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HZO05680.1"
                     /translation="AGYVLVTNNGWNLDSVLSAFVAGRALFRVRVNEALGFIVLATVF
                     GLIEKSVWGLVAAAIGSSAIALVHRVIAVRPFLRGRLTRAEYRTGLGYLPDLLSFGLR
                     ATPGQIAQGISNQGGIWAIGIVAPAAAVGAYSRAMVIPKSVQQASMKITEVLFPTLVA
                     RRAEGDGHGFDRALIDSIRYEAIGMGLLAAALGGCAHLVLEIFGPGFDAAATALVLLL
                     FFPVLAAISITQTQALWAVDRPGLTSVISVVRMIVTVALLVALTPGMHYAGPPLALLA
                     GMLVGIAISGWHLRSHLARPARATWPWRERVALVAAYAAGFGAAHGVEAAWHSLVALP
                     LALAAGCLAFALVLVLVGGINERDRERLAPLWARLRLRRVASFASIFLALLLLPASGA
                     GAYVYWSDGAGSGIGRAELTGAAAPGGGWLDAGGESCGVAVDAAHVYWTTRHGTIGRA
                     GLDGSGVDPEFIELPGGSACGVAVDAGHLYWASATSGKLGRAALDGSAVEPAWMSPGR
                     GHGCGIAVDAGRVYWASDSGVYSAPLAGGPTATISTATADNCGVAVNAVHIYWATREG
                     PGGEGVIMRGLLGGGPPTAIAAAPQSPCGVAIDSRYVYWANAGSDAISRAGLDGSGLV
                     KRFATGAAGPCGVAVDGFGAAAAPPSAARPSNLFRLGEVSKNRKRGTARLEVLVPGPG
                     TLVLVGHKVRERRLTRKAPGAGAVEISVRPRHATRELLREFGLAVAPLSVTYTPTGGV
                     PRTRYTRVWLRLSR"
BASE COUNT         1248 a         3108 c         3167 g         1168 t
ORIGIN      
        1 gcgagcgcga gcgccgcggt cccggagttg acgccgacac agtggccgac gccgcagtag
       61 gcggcgaact cctcctcgaa gcgctcgact tcgggaccga gcacgaagcc gccgtcggcg
      121 aggacgtcgt cgaaggcccg ccgcagctcc ccggcgatcg gccggtggtg gcgctccaag
      181 ctcatgaacg ggaccggggc gagggtggtc tcgctctcag tgctcatgcc gggcatcgcg
      241 gcgcaggcta ggtctgggtt ttcacagggg tgtttgaatc ttgtttcttt tccgctcggc
      301 ggcgacgaag cccgaaaaat gtgcgtgagc gtgaacagtt ttgagtgttg gtgtgggtca
      361 gttccggccc gttttcactt cctcttctca acctgttcac atcccaccgc cccccacccg
      421 agatcgtggc ccgatcgcct cccggggcgc ctgtggccat gtccaaccct cgcctcgcca
      481 aagcaaagaa agagagctcc ttcctgcggg gggccaagga gcgcctggcc ccgatcggcc
      541 gcttcctggt caaggacaag ctctcgacct tcctgctggt tgcctcgatc gggctgctgg
      601 tcctcttctt ctctttgctc ggttccctcg gccccgaggg cgaggggacc aaggtgccgc
      661 tctcgacgat ctccaacgtc ggcgagtcgg ggcggatcgc gaccgccgag ctcttggact
      721 acgaccacca ggtgatcgcc cacaccgagc tcggcctcga ctactacgcc gactacccgg
      781 cctctgacgc tgcaacccag cagctctatg accagctgag cggtggcggg gcctcggtct
      841 cggtcgaccc ccagtcgggc aaggccgaac ggaccgtggt cgtccagttc ctcctgccga
      901 tcctgatctt ggtctgcctg tttgccttct tcatccgcca gaccggggat ggctccgggg
      961 ggatcggcgc cttctcggtc ttcgggggca aaggcaagag acgaaagaag ggcgagaagg
     1021 cgtcgatcac cttcgactcg gtcgccggcg ccggggaagc cctggccgag ctgaaggaga
     1081 tccgcgactt cctcgatgac ccgaccaaat acctcgccct cggtgccgcg gcccccaagg
     1141 gggtcctctt ggtcggcccc ccggggaccg gcaagaccct gcttgccagg gccaccgccg
     1201 gggaggcgga cgccgccttc ttctcggcct cgggggcgga gttcgtcgag tccctggtcg
     1261 gggtcggggc ggcgaggatc cgcgacctct tcgccaaggc gcgcaagatg agcccggcga
     1321 tcatcttcat cgacgagctc gacgccgccg ggcgaaagcg cggagcgggc gtcgggcagg
     1381 gcaacgacga gcgcgagcag accctgaatc agctcctggt cgagatggac ggcttcggtg
     1441 gcgatgcggg ggtggtggtg atgggcgcga ccaaccgccc cgacatcctc gatcccgccc
     1501 tgcttcgccc cgggcgcttt gaccgccagg tggtgatcga cgtgcccgac gtgcacgggc
     1561 gctatgaaat cttggagctg cacgccaaag ggcgctcctt tgccgccggg gtggacctct
     1621 cggagatcgc caagctctgc ccgggcttct cgggtgcgga gctggccaac ctggtcaacg
     1681 aggccgccct gctcacggtc agggaaggca ggagcgagat cgaccagccg accctggagg
     1741 aggcgatcga ccgggtggtc gccggcccgg cgaagaagag ccacgtcttg agcgaggacg
     1801 agcgctggac gatcgccgtg cacgaggccg ggcacgcggt cgcgacccgc tcgatcgggc
     1861 agaccgtctc cgcccagaag ctctcgatcg tcgcccgcgg gcgccagctc ggcaccgccg
     1921 cccacatgct caccgaccgc gatgcggtga tgcacgccca gtctgacctg gagcgccagc
     1981 tggtcgcgat cctctcgggg accgcggcgg agtgggcgga gttcggcgag ggctccaccg
     2041 gggcctctga tgacctgcac gccgcgacca agctggcaag gcagatggtc acctccttcg
     2101 ggatgagccc gcgccttggc tcggtgacga tcggggaggc cgggggcgag gtcttcctcg
     2161 gggcctccct gcaggacatg ggctcggtcg ggccccacac cctggacgtg atcgacgaag
     2221 agaccgagcg gatcgtcgag gaggcgaagg ccagggccct gttcgtcttg cggggcaact
     2281 gggaggcggt gcaggagatc gccagggcgc tgctcgagca ggagacgctc tcgggggttg
     2341 cgctggaggc gttgctctcg acggtggcgg agttcgagct gggggagatt gcgttgccgg
     2401 gacctgaggt gcgttccgat ggcgaggcct gaggcctcag tcccagccga actcgcgccc
     2461 gaccagttcg tagaggcgcg cgttgtcggc ggcgagctcg gcccgcaggc gggcgcgggc
     2521 ggccggctcg atcggcgcgt aggagcgcgc gttcttggcc gcggtgtcgg ccggcgccag
     2581 tcgctccagc ccgaggaaga cctgcgcctc ggcagccgtc gagaccgggt cggcgaacag
     2641 gtcctcggcg ctcaggacca ggacccgctc acgcgggtag cactcgagcc agcgttcgag
     2701 ctggccggcg tagcgcccac gctcgaggta ggagtggtgc tggtgggcga acgagcggta
     2761 gcccggctcg gcgatcagcc gctcctcctc cccaccgagc cgcgcggctt ccgccgccag
     2821 cgccgcctcg aagtcgagct gctcgaagcc gagcacccgc tcgtggttgt gctgggagaa
     2881 ggcgcggtcg atcgggtcgc gcaggagcac gatcaggcgg gcgtcgggca cgagctcggc
     2941 ggcgcggcgc ggggccagcg ggtggaagag gtagtagggc gtggcctcgc cgctgaccgt
     3001 cgccgcgcgg gcgcgcggga agtgggcgcg gtaccagtcc gggccgcggg cgaactcgtg
     3061 atcgaagaag tgaacctcct tgcgcaccgg cgccgccaca cccgggtgct ggcagagcag
     3121 gtcgagcaac gaggtcgtcc cggccttctg cgcgccgacg atcaggaagc ccggcagcgg
     3181 ccggctgccg gcactggccc ggcgcagcgc gtgttcgccg gaacggcggg cgcggccgag
     3241 atgtcgtcgt ccgatctccg tggggctcgc catgcgcggg cggaaaggta caggtcgctt
     3301 tgagtcaggg tgctaacgag ccgcgaacgg tcgccccgcc gccccccgcc gtgagcgtga
     3361 tgcgagcgat gctctagaac tggtggatcg tgctgatcgc ggtcgtgatc ttcgccggga
     3421 tcggcgccac cctggcgctg gtccgcaccc ccgactacac ggcgaccgcg cggctctcgg
     3481 taggccgcat cgacatcacc tcgcccggcg cgctctccgg cttcgcggtc gccacccagg
     3541 cgctcgccac cggctacagc cgcaccgtca cctcgcaggc ggtggccacg gaggtctcgg
     3601 agaagaccgg gatctcggtc aaggacgtgc agagccacgt gaccgcgacg ccgatcgccg
     3661 aaagcccggt cttccggatc gaagcgacct cgcccgaccg cgagcaggcg atcgcgctcg
     3721 ccaacctcag cggccaggcg ctgatccgct acgccgcgaa gctgaaccgc gacaaccccg
     3781 acaccgcccg cctgttcgcc aagtaccggc aggcggccgg cgacgcgacg aacgccgagc
     3841 aggagctgga agcggccgaa gaagacctcg aagagcgtga gatcccgccg gtcgaaaagc
     3901 aggtcgaccg cgccgaggcc gacctcgagg cggccgaact gcgcgccgag gccttcggcc
     3961 gggcctacac ctcgagcgtc cagagtcagg tcgcaaccga cctcgtgcag atgatctcgc
     4021 cggcgatcga cgccgagagc gacaagcgct cgaccttctt catctacgcc ttcatcggcc
     4081 tcgtcatcgg cctcttcgtc ggcggcgcgc tcgcctactg gcgcgagtgg cgactcagcg
     4141 tcggcgccgc ctcccgcgcc cgcgaggccg agtccggcta gccgtcggga tgcaggtcca
     4201 gcttcagcac ctgcaaccgc tcccagggga acgaggccaa gagctggtgg gggtagcgcc
     4261 cttcgaagta gccgttcggc ccggtgtagg ggttcttcgc cgcggtcacc gggtcctggg
     4321 cgaagagctg gacgccgccg gcgccatcgc ggacgaagat gccgtagcgc tgcgccgctt
     4381 cggcgatcag acgggtgacc cgcggcatct gcagcttcgc caggtcgagc ttcgggtcga
     4441 ggcgcagccg ggcgccctcg ggcagcgcca gcgggctggc cgatttgccg tcggtgcgct
     4501 gggcgggcgc ggcgtagacg ccggcgcgga tctcgggcag ggcgatcgcg agcgcgtggt
     4561 cgatctcgcc ctcgcgcagg tcctcgaggg tgatcagccc gccggcgacc ggcagggagc
     4621 tggccgaggc gccccaccag ggctcggcgt cgggccaggc gtcggtgtcg tagacgccgc
     4681 ggttggcgga gacgttctcc atcgccccgc cccaggaggc ggagggttcg gcgccgggga
     4741 cgagccgcca gaactcccac atgcggtcgg tcgagggctg ccagacgacc aggtggccgt
     4801 cggtgccgcc ggagggggtc gcctcgggcg gcagcggcac tcggtcaaat gccgcctgca
     4861 gcggcggcga gaagatcggg ctggtcagct ttacccgaac cgcgggctgg ctcgccggga
     4921 cggtgtagat cggcacgctg tagcggctgg tgccgatcca gggtccggag gcgtcagcga
     4981 gctcggcctc gacctcggtg ctcagcgccc gcaccagctt cgccgagcgc tggtccagct
     5041 tcgcggcggc ggggaccggc gcgttccaga agctcgccgg cgagaagaag cggaagtggt
     5101 cggcggaggc cttggccttg ctgcccttgc gcgcggcgac gccggcggcg tccgccccgg
     5161 ccggcgggac cagcagcaag aggagcacgc aggcgagcgc cgccgcgatc agcgcggcgc
     5221 ggactttgcg ggtcggggtc ttggcgagcg aacggggcat gcgtgacttg gtgtcggcca
     5281 gatgcccgct ccgccgtgcg ccccaagcgt cgtttggacg tttgtccatc gcgtctgcaa
     5341 gtgaggctgc tcctggccct gtcctgcctg gcggcgttgg ccggcggctg cggctccgac
     5401 gcgctgccgg aggggccgac cggaccggcc ccgaaaggcg agcaaagcac cgctttccgc
     5461 ttcttctcgc cggctagctt ctggaacacg ccggtgccgg cgggtgccgc gatcgacccc
     5521 ggatcgcccc ggttggtgcg cgcgctcgac actgaggtcg ccgccgagca ggcggcggcg
     5581 cgcgggccct ggatcagcac cacccgctac agcatcccga tctacaccgt gcccgccgag
     5641 cagccgaccg tgcgggtgcg gctgaccagc ccggtccact ccgccgccct gcaggcggcg
     5701 ttcgaggcgg tgccgctgcc gcccggcgcc tacccctcgg gcggaaccga cgggcacctg
     5761 gtcgtctggc agccgaagac cgacaggctc tgggagttct ggcggctgcg ccgccgaccg
     5821 gacggcccct cggcctcttg gggcggggcg atcgaacggg tctccgagag caccggcgtc
     5881 tacgacgccg acgtctggca caacgccgag acctggtggg gagcctcggc cagctcgctc
     5941 tcgatcgccg gcggcctgat cacgctcgac gacctgcgcc acggcaagat cgaccacgca
     6001 ctcgccctgg cgctgccgca gatccgggcc ggtgtctacg cctccccggc gcagcgcacc
     6061 gacggcaagt cggccgaccc ccgatcccta cccgagggcg cccacctgcg cctcgacccg
     6121 gacctcgacc tcgactcgct gccgatgccg ccgctgaccc ggatgatcgc ggcggccgcc
     6181 cagcgctacg gcctcttcgt ccgcgacggc tccgccgtcg tccagctctt cgccgaggac
     6241 ccggtcaccg ccccccacaa cccctacacc ggcccgaccg gctacttcga aggccgctac
     6301 ccgaacgagc tgctcgcctc cttcccctgg caccgcctcg aagtcctcga catggacctc
     6361 cgccgggacc gttagccctc tcgacatttc gggcgctatg acgcccacaa agtcgagagc
     6421 gcgagctaac ggctgaggcg cagccaaacg cgggtgtagc gggtgcgggg aacgccgccg
     6481 gtcggggtgt aggtgacgct gagcggggcg acggcgaggc cgaactcgcg cagcagctcg
     6541 cgggtggcgt ggcgcggcct gaccgagatc tcgaccgcgc cggcgccggg cgccttgcgg
     6601 gtgagccgac gttcgcgcac cttgtggccg accaggacca gcgtgcccgg tcccggcacc
     6661 agcacctcga gccgcgccgt cccgcgcttg cggttcttgc tcacctcgcc gagccggaag
     6721 aggttcgagg gacgggcggc ggacggcggc gcggcggcgg cgccgaagcc gtcgacggcg
     6781 accccgcagg ggccggccgc gcccgtggcg aagcgcttga ccaggccgga gccgtcgagg
     6841 ccggcgcggc tgatcgcgtc ggagccggcg ttggcccagt agacgtagcg cgagtcgatc
     6901 gcgaccccgc agggcgactg cggcgcggca gcgatcgcgg tcggcgggcc gccgccgagc
     6961 aggccgcgca tgatcacccc ctctcccccg ggtccctcgc gcgtcgccca gtagatatgg
     7021 acggcgttga cggcgacgcc acagttgtcg gcggtcgcgg tcgagatcgt cgcggtcggg
     7081 cccccggcca gcggggcgct gtagacgccg ctgtcgctgg cccagtagac gcggccggcg
     7141 tcgaccgcga tcccgcagcc gtggccgcgg ccgggcgaca tccaagccgg ttcgaccgcg
     7201 ctgccgtcga gcgccgccct gcccagcttg ccgctggtcg cgctcgccca gtagaggtgg
     7261 ccggcgtcga ccgcgacccc gcaggccgag ccgcccggca gctcgatgaa ctccggatcg
     7321 accccgctgc cgtcgagccc ggcgcggccg atcgtgccgt ggcgcgtcgt ccagtagacg
     7381 tgcgcggcat cgacggcgac gccgcagctc tcgccgccgg cgtcgagcca gccgccgccc
     7441 ggcgccgccg caccggtcag ctcggcgcgg ccgatcccgc tgccggcgcc gtcggaccag
     7501 tagacgtagg cgccggcgcc gctcgccggc aagagcagga gggcaaggaa gatcgaggcg
     7561 aaggaggcga cccggcgcag ccgcagccgc gcccagagcg gggcgaggcg ttcgcggtcg
     7621 cgctcgttga tcccgccgac caggaccagg acgagcgcga aggcgaggca gccggcggcg
     7681 agcgccagcg gcagggccac gagcgagtgc caggccgctt cgaccccgtg cgcggcgccg
     7741 aacccagcgg cgtaggccgc gaccagcgcc acccgctcgc gccacggcca ggtcgcccgc
     7801 gccggccgcg ccaggtggct gcgcaggtgc cagccggaga tcgcgatccc gaccagcatc
     7861 ccggcgagca gcgccagcgg cggcccggcg tagtgcatgc ccggggtcag cgcgaccagc
     7921 agcgccaccg tgacgatcat gcggacgacc gagatcacgc tggtcagccc cggccggtcg
     7981 accgcccaga gcgcctgcgt ctgggtgatc gagatcgcgg cgaggaccgg gaagaagagc
     8041 agcaggacga gcgcggtcgc ggcggcgtcg aagccggggc cgaagatctc gaggaccagg
     8101 tgcgcgcagc cgccgagcgc cgccgccagc agccccatcc cgatcgcttc gtagcggatc
     8161 gagtcgatca gggcgcggtc gaagccgtgc ccgtcgccct cggcgcggcg cgcgaccagg
     8221 gtcgggaaca gcacctcggt gatcttcatg ctcgcctgct ggacgctttt cgggatcacc
     8281 atcgcgcggc tgtaggcgcc gacggcggca gccggggcga cgatcccgat cgcccagatc
     8341 cccccctggt tgctgatccc ctgggcgatc tgccccggcg tcgcccgcag cccgaagctg
     8401 agcaggtcgg gcaggtagcc gagcccggtg cggtattcgg cccgggtcag ccggccgcgc
     8461 aggaacggcc gcaccgcgat cacccggtgc acgagggcga tcgccgagga cccgatcgcc
     8521 gccgcgacca gcccccagac cgacttctcg atcagcccga agacggtggc gaggacgatg
     8581 aacccgagcg cttcgttgac ccgcacgcgg aacagcgcgc gcccggcgac gaaggcggag
     8641 agcaccgagt cgaggttcca gccgttgttg gtgaccagca cgtagccggc g
//
LOCUS       DASRIG010000175         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3204871, whole genome shotgun sequence.
ACCESSION   DASRIG010000175 DASRIG010000000
VERSION     DASRIG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204871"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFB52_04785"
     CDS             complement(<1..673)
                     /locus_tag="VFB52_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZO05681.1"
                     /translation="MATERAAESNQQGIKSIEIGARVLLALEQGRGPATLSEVAKAAG
                     LHPAKAHRYLTSLVRTGLASQDATTNMYDLGPAARHLGVEALRRVDSVRVAQTRITEL
                     RDATGHTVNVSVWSEYGPTMVSWDTGAHLLPMVIRVGSTLPLLDSAVGYVFLAHLADS
                     MTADVIRSQQEQGATRTISAAEVEELKAQIRSEGYGRTRNQMIFGLAALSAPVFGPDG
                     QLELAI"
     gene            complement(692..2233)
                     /locus_tag="VFB52_04790"
     CDS             complement(692..2233)
                     /locus_tag="VFB52_04790"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZO05682.1"
                     /translation="MSAVTHDLAIVGYGPTGMTLAALAGQQGHSVVVLERYAGLYNLP
                     RAACFDDEIMRTFQKLGVEQEVLPGAVAQHDYDWVNAAGETLVEISYDDPAPGGWAQL
                     YMMYQPHIETVLDRLCRSLPSVEVHQGVTVEAMEADEEGVDVRGVGPDGEEIAVRASW
                     LVGADGGKGFVRPALGIDFSTYGFEENWLVCDFERRKELTWVPTFRQVCDPAQPIAIV
                     RIGPDHQRFSFMLEPDEDPEIALDPERVWARVRDYITPEDADLIRVANYVFRSRITDS
                     WREGRVLLAGDAAHEMPPFLAQGMCSGMRDAHNIAWKLDLILNGVAGEDLLDSYQPER
                     EPHVRFITEKAIELGRVQTLRDPEKARERDERFLAMRRENKAPEKLRYPALDGGALTA
                     LGGAHAGEFFVQGRIRSATVTDGLFDDVVGQGPCVLVRDETLLDELEPEQREGWRRLG
                     GHFAVVDAETDLDGTYAAWFDAHDCAAAVVRPDWYLFGTAADGEELGRLLGAIDGVLS
                     PPVAT"
     gene            complement(2241..>3098)
                     /locus_tag="VFB52_04795"
     CDS             complement(2241..>3098)
                     /locus_tag="VFB52_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO05683.1"
                     /translation="GDCAGTVEEVGEGVDADLVGRRVTASATQTCGQCEQCRIGRHER
                     CAQLSLLGVHREGSYADLAVVDAGVVRPIPDELSMSEAAALAADGAIAFHQLETAGAV
                     AGEHVLVTGATGALGTTLCALGPALGAEVIGLARRPDAIPAELNLVARLDANDPELTE
                     KLMAVSGPGGVAVAIDNVANPEAFSRYFMALAIGARVVFSGAIATPEGLPVLPVPAVP
                     LYVKSISLLGTRTSARATDDRFWHLVADGFRLPPGLIHELPLERAAEAHAMIAAGTQL
                     GHTVLTLGD"
BASE COUNT          408 a         1087 c         1160 g          443 t
ORIGIN      
        1 cgatcgccag ttcgagctgg ccgtcggggc cgaagaccgg ggcgctgagc gcggcgaggc
       61 cgaagatcat ctggttgcgg gtgcggccgt agccctcgct gcgaatctgc gccttcagct
      121 cctcgacctc ggccgccgag atcgtccgcg tcgcgccctg ctcctgctgg gagcggatca
      181 cgtcggcggt catcgagtcc gccaggtggg cgaggaagac gtagccgacc gcggagtcga
      241 gcagcggcag ggtcgagccg acccggatca ccatcggcag caggtgcgcg ccggtgtccc
      301 aggagaccat cgtcggcccg tactcgctcc agaccgagac gttgaccgtg tggccggtgg
      361 cgtcgcgcag ctcggtgatc cgggtctggg cgacccgcac cgagtcgacc cggcgcagcg
      421 cctcgacccc gaggtggcgg gcggccggcc cgaggtcgta catgttcgtg gtcgcgtcct
      481 gggaagcgag cccggtgcgg accaggctgg tcaggtagcg gtgcgctttc gccgggtgca
      541 ggccggcggc tttggcgact tcggagaggg tcgcgggccc gcgaccctgc tcgagcgcga
      601 gcagcacacg ggcgccgatc tcgatcgact tgatcccctg ctggttgctc tcggcggccc
      661 gctcagttgc catcgaccgg gccatcatct ctcaagtggc gaccggaggg ctaagcaccc
      721 cgtcgatcgc gccgagcagc cggcccagct cctcgccgtc ggccgcggtc ccgaacaggt
      781 accagtccgg gcgcacgacc gcggcggcgc aatcgtgggc gtcgaaccag gcggcgtagg
      841 tgccgtcgag gtcggtctcg gcgtcgacca ccgcgaagtg gccgccgagc cggcgccagc
      901 cctcgcgctg ctcgggctcg agctcgtcga gcagcgtttc gtcgcggacg agcacgcagg
      961 ggccctggcc gaccacgtcg tcgaacagcc cgtcggtgac cgtggcgcta cggatccggc
     1021 cctggacgaa gaactcgccc gcgtgggcgc cgccgagcgc ggtcagcgcg ccgccgtcga
     1081 gtgccgggta gcgcagcttc tccggtgcct tgttctcgcg ccgcatggcc aggaagcgct
     1141 cgtcgcgctc gcgcgccttc tcggggtcgc gcagggtctg cacccggccc agctcgatcg
     1201 ctttctcggt gatgaagcgg acgtgcggct cgcgctcggg ctggtagctg tcgagcagat
     1261 cctcgcccgc cacgccgttc aggatcaggt cgagcttcca ggcgatgttg tgggcgtcgc
     1321 gcatccccga gcacatcccc tgggcgagga agggcggcat ctcgtgggcg gcgtcgccgg
     1381 ccagcagcac ccggccctcg cgccagctat cggtgatccg cgagcggaag acgtagttgg
     1441 cgacccggat caggtcggcg tcctcggggg tgatgtagtc gcggacccgc gcccagaccc
     1501 gctcggggtc gagcgcgatc tcggggtctt cgtcgggctc gagcatgaac gagaagcgct
     1561 ggtggtcggg gccgatgcgg acgatcgcga tcggctgggc cgggtcgcag acctggcgga
     1621 aggtcggcac ccaggtcagc tctttgcggc gctcgaagtc gcagaccagc cagttctctt
     1681 cgaacccgta ggtggagaag tcgattccga gcgccggccg cacgaagcct ttaccgccgt
     1741 cggcgccgac cagccagctg gcgcggaccg cgatctcctc gccgtcgggg ccgacgccgc
     1801 gcacgtcgac gccctcctcg tcggcctcca tcgcctcgac cgtgacgccc tggtggacct
     1861 cgacgctcgg cagcgatcgg cagagccggt cgagcacggt ctctatatgg ggctggtaca
     1921 tcatgtagag ctgcgcccag ccgcccgggg cggggtcgtc gtaggagatc tcgaccaggg
     1981 tctcgccggc ggcgttgacc cagtcgtagt cgtgctgggc gaccgcgccg ggcagcacct
     2041 cctgctcgac cccgagcttc tggaacgtgc gcatgatctc gtcgtcgaaa caggcggcgc
     2101 gcggcaggtt gtagaggccg gcgtagcgct cgagcacgac gacgctgtgg ccctgctggc
     2161 cggccaacgc cgccagggtc atcccggtgg gtccgtagcc gacgatcgcg aggtcgtggg
     2221 tcacggcgct catcgcgcgc tcagtccccg agggtgagga ccgtgtggcc gagctgggtg
     2281 ccggcggcga tcatcgcgtg cgcctcggcc gcccgctcca gcggcagctc gtggatcagc
     2341 ccggggggga ggcggaagcc gtcggcgacc aggtgccaga agcggtcgtc ggtggcgcgg
     2401 gccgaggtgc gggtgccgag cagcgagatg cttttgacgt agagcggcac cgccgggacc
     2461 ggcagcaccg gcaggccctc cggggtggcg atcgcgccgg agaagacgac gcgggcgccg
     2521 atcgcgaggg ccatgaagta gcgcgagaag gcctcggggt tggcgacgtt gtcgatcgcg
     2581 accgcgaccc cgcccgggcc actcaccgcc atcagcttct cggtcagctc cgggtcgttg
     2641 gcgtcgagcc gcgcgaccag gttcagctcg gcggggatcg cgtcgggccg gcgggcgagg
     2701 ccgatcacct cggcgccgag cgccgggccg agcgcgcaga gcgtcgtccc cagcgccccg
     2761 gtggcgccgg tgacgagcac gtgctcgccg gcgaccgcgc cggcggtctc gagctggtgg
     2821 aaggcgatcg cgccgtcggc ggccagcgcc gccgcctcgc tcatcgagag ctcgtcgggg
     2881 atcgggcgga cgacgccagc gtcgaccacg gccaggtcgg cgtaggagcc ctcgcggtgc
     2941 acgccgagca ggctgagctg cgcacagcgc tcgtggcgcc cgatccggca ctgttcgcac
     3001 tggccgcagg tctgcgtcgc cgaggcggtc acccgccgcc cgaccaggtc cgcgtcgacg
     3061 ccctcgccga cctcctcgac cgtgccggcg cagtcgcc
//
LOCUS       DASRIG010000176         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2707462, whole genome shotgun sequence.
ACCESSION   DASRIG010000176 DASRIG010000000
VERSION     DASRIG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2707462"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFB52_04800"
     CDS             <1..319
                     /locus_tag="VFB52_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZO05684.1"
                     /translation="NVAAGDLGAPAAKKYDCEAWIPSQERYRELTSCSNTTDYQARRM
                     ACRYRPAEGEAPVAVHTLNGTACAVGRTIIALIENRQERNGGFSLPNTLQKFGSPDTI
                     APL"
     gene            316..969
                     /locus_tag="VFB52_04805"
     CDS             316..969
                     /locus_tag="VFB52_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05685.1"
                     /translation="MNSSVREAKRVVLCDDHEIVREAIKARMQNSSIVEIVGEAGNGR
                     DVVDVVKDLEPDVLIIDVELPKRDGIEATKEVLKVRPRTKVIIFTAHAQPDLLALALR
                     AGASGYVLKSAPAEDIARAVKAVTGGGTFLGGDFAGGKPSEVEKLLDLSPRERQILEL
                     LAEGLRVKEIAERLALSPATVHTHVRNAIAKLEVDTRTEAVALAVRFSYLGAGGGAL"
     gene            complement(970..1686)
                     /locus_tag="VFB52_04810"
     CDS             complement(970..1686)
                     /locus_tag="VFB52_04810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO05686.1"
                     /translation="MSRRPSDIPPSRAPVGSTLLVAGLVAIALGLLLPLAIGSFSDRQ
                     TLVLALVEAACLVVVAVGVVVMARRLRASHDALWSLARRDELTGVGNYRGLHERLSEA
                     IARHRRHGRQFALILLDLDGFKEVNERFGHLEGDRLLTEVGQALSDEVRGEDAVFRQG
                     GDEFAVIVEETDAEEALEVAARIRARVANRGYDSDADRPLTGATGVAIFPADGVDAEQ
                     LLGFADHDLFAAKRGEADAV"
     gene            1796..>3273
                     /locus_tag="VFB52_04815"
     CDS             1796..>3273
                     /locus_tag="VFB52_04815"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZO05687.1"
                     /translation="MRYPVEQFSPEERAVLAPHFTNLDRPVFALVNLPETVKGALFAR
                     YSRYSGTVRRLYLEEFANDAPTGGRPFDAEEGARAAQLYERVFVGYGDDSIAQVGGAH
                     IACEWVSNIVTKLLQRGRLAAYLEQSTRYIPYDVPLPEGGYRYYSDEQLGPEFGAAMD
                     ELFGIYSRSLTAVEEWAAERWPRGDGEPEGPWQRSIRAKALDLLRGLLPAATLSHVGI
                     YASGQAYEQLILRLLASPLPEAREYGGMILAELQRVMPSFVSRVERPDRGGEWISYLR
                     QRRERTESWVARLGLDRRGEEEAAPSVELLHVDGSEEDLLASCLFEAASASETEVLAR
                     IDVLDSEERAQLLAALAGDRANRRHRPGRGFEALRYRFEIVSDYGAFRDLQRHRMLTC
                     QWQRLTPDLGADVPEEVVEAGVGGEFERALEISQGEYERLATAGLTELAPYALSLAFR
                     IRYTLDLNAREAMHLIELRSGREGHPGYRAVAHALHAEIGRV"
BASE COUNT          498 a         1191 c         1126 g          458 t
ORIGIN      
        1 caacgtcgcc gccggcgacc tcggcgcccc ggccgcgaag aagtacgact gcgaggcctg
       61 gatcccgagc caggagcgct accgcgagct gacctcgtgc tcgaacacga ccgactacca
      121 ggcgcgccgc atggcctgcc gctaccgccc cgccgagggc gaggcgccgg tcgccgtgca
      181 cactctgaac ggcaccgcct gcgccgtcgg gcgcacgatc atcgccctga tcgagaaccg
      241 ccaggaacga aacggtggat tctccttacc aaatactttg cagaagttcg ggtcgcctga
      301 cacaatcgcg cccctatgaa ctccagtgtg cgcgaggcca agcgggtggt cctctgcgac
      361 gaccacgaga tcgttcgcga ggcgattaag gcccggatgc agaactcgag catcgtcgag
      421 atcgtcggcg aggccggcaa cgggcgtgac gtggtcgatg tggtcaagga tctcgagccc
      481 gacgttctga tcatcgacgt cgagctgccc aagcgcgacg ggatcgaggc gaccaaggag
      541 gtcctgaaag tccggccgcg gaccaaagtg atcatcttca ccgcgcacgc ccagcccgac
      601 ctgctggcgc tggccctgcg cgcgggcgcc tccggctacg tgctcaagtc ggccccggcc
      661 gaggacatcg cccgcgcggt caaagcggtg accggcggcg gcaccttcct cggcggtgac
      721 ttcgccggcg gcaagccctc cgaggtcgag aaactgcttg acctctcacc gcgcgagcgc
      781 cagatcctcg agctgctggc cgagggactg cgggtcaaag agatcgccga gcggctggcg
      841 ctctcccccg ccaccgtgca cacccacgtg cgcaacgcga tcgccaagct cgaggtcgac
      901 acccgcaccg aggcggtcgc gcttgcggtc cgcttcagct acctcggcgc cggcggcggc
      961 gccctctagc tagaccgcgt cggcttcgcc gcgcttggcg gcgaagaggt cgtggtcggc
     1021 gaagccgagc agctgctcgg cgtcgactcc gtcggcggga aatatcgcga ccccggtggc
     1081 accggtgagc ggccggtcgg cgtcgctgtc gtagccgcgg ttggcgaccc gggcgcggat
     1141 ccgcgccgcc acttcgagcg cctcttcggc gtcggtctcc tcgacgatca cggcgaactc
     1201 gtcgccgccc tggcggaaga ccgcgtcctc gccgcgcacc tcgtcgctga gcgcctgccc
     1261 gacctcggtc agcagccggt cgccctcgag atggccgaag cgctcgttga cctccttgaa
     1321 gccgtcgagg tcgagcagga tcagggcgaa ctggcggccg tggcggcggt ggcgggcgat
     1381 cgcctcggac aaccgctcgt gcaggccccg gtagttgccc accccggtca gctcatcacg
     1441 gcgggcgagg ctccagagcg cgtcgtggct ggcccgcagc cgccgcgcca tcacaaccac
     1501 accgaccgcg acgacgacca ggcaggcggc ctcgaccaag gccaggacga gcgtctggcg
     1561 gtcggagaag gagccgatcg ccagcggcag gagcagcccc agggcgatcg cgacgagccc
     1621 ggcgaccagc agggtcgacc cgaccggcgc gcgggaaggc gggatgtcag aaggccgtct
     1681 gctcatagtc acttttaggt atcgattcca accctacccg cacggtgcgg ggtcgatgcg
     1741 aggaggttca ggggccattg gcgactcgtc ccaacgcctg attacgatgc cccaggtgcg
     1801 ctacccggta gaacagttca gccccgagga gcgagcggtc ctcgctcccc acttcaccaa
     1861 cctcgatcgt ccggtgttcg ccctcgtaaa cctgccggag accgtcaaag gtgcgctttt
     1921 tgctcgctac tcgcgctatt cggggacggt gcggcggctc tacctggagg agttcgccaa
     1981 cgacgcgccg accggcggtc gccccttcga cgccgaggag ggggcgcggg cggcgcagct
     2041 ctacgagcgc gttttcgtcg gctacggcga cgactcgatc gcccaggtcg gcggcgccca
     2101 catcgcctgc gagtgggtct ccaacatcgt caccaagctg ctccagcgcg gccggctcgc
     2161 cgcctacctc gagcagtcga cccgctacat cccttacgac gtaccgctgc ccgaaggcgg
     2221 ctaccgctac tactccgacg agcagctcgg gcccgagttc ggcgcggcga tggacgagct
     2281 gttcgggatc tactcgcgct cgctgaccgc ggtcgaggag tgggcggccg agcgctggcc
     2341 gcgcggcgat ggcgaaccgg agggtccctg gcagcgctcg atccgggcca aggcgctcga
     2401 cctgctgcgc ggcctcctgc cggcagcgac gctcagccac gtcggcatct acgcctccgg
     2461 ccaggcctac gagcagctga tcctgcgcct gctcgcctcg ccgctcccgg aggcgcgcga
     2521 gtacggcggc atgatcctcg ccgagctgca gcgggtgatg ccgagcttcg tctcccgggt
     2581 cgagcgcccc gaccgcggcg gcgagtggat ctcctatctg cgccagcgcc gcgagcggac
     2641 cgagagctgg gtcgcgcggc tcgggctcga ccggcgcggc gaggaggagg cggcgccctc
     2701 ggtcgagctg ctgcacgtcg acggctccga agaggacctg ctcgcctcct gcctgttcga
     2761 ggcggcgagc gcctcggaga ccgaggtcct cgcccgcatc gacgtgctcg acagcgaaga
     2821 gcgggcacag ctgctggcgg cgctggccgg cgatcgcgcc aaccgccgcc accggcccgg
     2881 ccgcggcttc gaggcgctgc gctaccgctt cgagatcgtc tccgactacg gcgccttccg
     2941 cgacctgcag cggcaccgga tgctgacctg ccagtggcag cggctcaccc ccgacctcgg
     3001 cgcggacgtg ccggaggagg tcgtcgaagc cggggtcggc ggcgagttcg aacgggcgct
     3061 ggagatctcg cagggcgagt acgagcggct ggccacggct ggcctgaccg agctggcgcc
     3121 ctacgcgctc agcctcgcct tccgcatccg ctacacgctc gacctcaacg cgcgcgaggc
     3181 gatgcacctg atcgagctgc gctccggccg cgagggacac cccggctacc gcgcggtggc
     3241 ccacgccctg cacgccgaga tcgggcgcgt tca
//
LOCUS       DASRIG010000177         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3477843, whole genome shotgun sequence.
ACCESSION   DASRIG010000177 DASRIG010000000
VERSION     DASRIG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477843"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFB52_04820"
     CDS             <1..272
                     /locus_tag="VFB52_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05688.1"
                     /translation="GDNAAAIQFGLEKTARVITGAAAIMLAVFIVFAGAPIATVSQLG
                     IGLTVAVILDATVVRIVLLPALMLLLGDRVWWLPKWLQGMPKVNA"
     gene            complement(306..1061)
                     /locus_tag="VFB52_04825"
     CDS             complement(306..1061)
                     /locus_tag="VFB52_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05689.1"
                     /translation="MASAIALLAAITASVALAGAPVTGADGNSQAIDVKIGPKTLSKT
                     KLTPATLEVTTKVTTSNPTGVPVPATRVVVDFDKTAKLFTKGLPTCTSAKLQNVSTEI
                     AEQNCKSAIIGRGTASALLPVGSKIFPVAQEVTAFNGVPKGGKPVVYLHSYGTTPIQT
                     TLVLTGVVSTYNKEGYGPRLDVEVPLIAGGAGALTDFNVKIDKKFRYKGEMRSFISAK
                     CPNSKKLKARGAFTFKDGETITALSSQSCKQKK"
     gene            1350..2678
                     /locus_tag="VFB52_04830"
     CDS             1350..2678
                     /locus_tag="VFB52_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO05690.1"
                     /translation="MIAALAAAVIVLAYIFLTGSGGHQYTLLFQNAGQLVPDNQVLIG
                     GSPVGSVESIELTDDNLAAINIEVDQELHEGTTAVIRATSLSGVANHYVSLSPGPNSN
                     PELDDGAELGLASTTTPIDIDQLFNTFPPSVRKGLSDFIKGNATIYAGQGENANEAYE
                     YFGPALNRTTAFTSELNADQRLLEKFIVSSAELTTAVAERGEELSSAIGNANAAFNAI
                     AAQNVAFDETLQELPPTFRQANTTFVNLRAALDDLDPLVETAKPATKNLAPFLAELRP
                     VLSKFIPVVRNLRLTVDRPGFANDAAELLATLPAVESRASKTFPHAEDAIAGFQPNLN
                     FARSYTTDLFNALGKLGAAAGYYDGNGHYVRASPSALNVFENSGGTLTPVERKNALQA
                     FGAEAGVRKPCPGGATQNATDGSSPYVNPPQTGSSVSPSECDPSDVPPGP"
     gene            2675..4195
                     /locus_tag="VFB52_04835"
     CDS             2675..4195
                     /locus_tag="VFB52_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO05691.1"
                     /translation="MKRAIPALLLVAVVVVVVLLLAGGGGSGNSYVVRAIFDNGAYLV
                     NGEQVRVAGANVGEIKAVGVTMPGDVVAYKDGEPVEKPGKAVIELEITDPGFQDFRED
                     ATCQIRPQSLIGEKFVDCRPTLPRAPGSEPPPPLKEIPEGEEGEGQHLLPLGSNGTSV
                     DPDLINNIQTLPYSQRFRLIFNELGAGFAGRGADLEVLVKRANPVLRDVDKLFKILSD
                     QREELAQLASDSNEILGPLARERRHVAGFISNAGAAAEASSERGEQLEEALDKFPTFL
                     REFRTTMTNLEGFSSAATPVMEDLGAAAPSLTDATRTLTPFSEALTVSLKSLGETGEA
                     SGPIFAEADPIIRKTTELSKSGVVPTRELAKMTTSLSQSGGWNGLVELIYNTSAAING
                     FDQYGHYGRTYVTLSTCTNYISGASGYSGGGCTSRFNGPNVGEPGEEPLMALLNMLNE
                     MKVDGSAGGTGVPLDQSGGPATGLGQADETEPEPEIGSSSQAAAGTEPLLDYLLGP"
     gene            4192..>4398
                     /locus_tag="VFB52_04840"
     CDS             4192..>4398
                     /locus_tag="VFB52_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05692.1"
                     /translation="MRNKGGISGLASNPILVGAVTVLVVIVAVFLAYNANNGLPFVST
                     YDLKARVPNADALVKGNEVRVGGVR"
BASE COUNT          758 a         1528 c         1417 g          695 t
ORIGIN      
        1 acggcgacaa cgccgccgcg attcagttcg ggctggagaa gaccgcgcgg gtgatcaccg
       61 gcgcggcggc gatcatgctc gccgtcttca tcgtcttcgc gggcgcgccg atcgcgacgg
      121 tcagccaact cgggatcggc ctgaccgtcg cggtgatact cgacgccacg gtcgtgcgca
      181 tcgtcctgct gccggcgctg atgctgctgc tcggcgaccg tgtctggtgg ctgccgaagt
      241 ggctgcaggg gatgccgaag gtgaacgcgt agcgcccggc gccgttcagc gccgggcgcc
      301 cgcagctatt tcttctgctt gcaggactgc gacgacagcg cggtgatcgt ttcgccgtct
      361 ttgaaggtga acgccccgcg ggctttcagc ttcttgctgt tcgggcattt ggcgctgatg
      421 aagctgcgca tctcgccctt gtagcggaat ttcttgtcga tcttgacgtt gaagtccgtc
      481 agcgcgccgg cgccaccggc gatcagcggc acttccacgt cgaggcgcgg gccgtagcct
      541 tccttgttgt aggtggagac gacgccggtg agcaccaggg tggtctggat cggggtcgtg
      601 ccgtaggagt gcaggtacac gaccggcttg ccgcctttgg gcacgccgtt gaacgcggtc
      661 acctcctgcg cgacggggaa gattttcgac ccgacgggca gcagtgccga ggcggtgccg
      721 cggccgatga tcgcgctctt gcagttctgt tcggcgattt cagtcgagac gttctgcagt
      781 ttggcgctgg tgcaggtggg caggcctttg gtgaacagct tcgccgtttt gtcgaaatcg
      841 acgacgacgc gggtggccgg caccgggacg ccggtcgggt tggaggtcgt gactttggtc
      901 gtcacttcca gcgtcgccgg ggtcagtttg gttttcgaaa gcgttttcgg gccgatcttc
      961 acgtcgatcg cctgcgaatt gccgtcggca ccggtcacgg gcgctcccgc cagtgccacc
     1021 gatgcggtga tcgcggcaag caaagcgatg gccgaagcca cgatcaagat tggacgtctg
     1081 tgcattgagc acctctcctc aagtatcgat tgggtgaggc gcctcccatt cccctaaacc
     1141 cctgccgcat gcagagtagc gcgttgattt ccagttaagt ccccgctcag gcagggcttc
     1201 gcggctggac cctcggatac gattcgcgcg ttcgttagcc ccgggaagga gggccggggg
     1261 gcgaacagcg acgacccgtg gcttccaatt ccttaggcga ggagaaaagc tcggctccga
     1321 gccgtgggac ttcccccgcc cggattgccg tgatcgcggc ccttgccgcg gcggtaatcg
     1381 tgctcgcgta catcttcttg accggcagcg gcggacacca gtacacgctc ctgttccaga
     1441 acgcgggcca gctcgtgccc gacaaccagg tcctgatcgg cggctcgccg gtcggctctg
     1501 tcgaaagcat cgagctgacc gacgacaacc tcgccgcgat caacatcgaa gtcgaccagg
     1561 agctgcacga gggcacgacc gcggtgatcc gcgccacctc gctctccggc gtcgccaacc
     1621 actacgtctc gctcagcccc ggtccgaaca gcaacccgga gctcgacgac ggggccgagc
     1681 tgggcctcgc ctcgaccacg accccgatcg acatcgacca gctcttcaac accttcccgc
     1741 cctcggtgcg caaaggcctc agcgacttca tcaagggcaa cgcgacgatc tacgcgggcc
     1801 agggcgagaa cgccaacgaa gcctacgaat acttcggccc ggcgctgaac cggaccaccg
     1861 ccttcacctc cgaactgaac gccgaccagc gcctgctgga aaagttcatc gtcagctcgg
     1921 ccgagctgac cacggcggtg gccgagcgcg gcgaagagct ctccagcgcg atcggcaacg
     1981 ccaacgccgc cttcaacgcg atcgctgccc agaacgtcgc cttcgacgaa accctgcagg
     2041 agctgccgcc gaccttccgc caggcgaaca cgaccttcgt caacctgcgg gcggcgctcg
     2101 acgacctcga cccgctggtc gaaacggcga agccggcgac caagaacctg gctccgttcc
     2161 tcgccgaact gcgcccggtg ctgtcgaaat tcatcccggt ggtccgcaac ctgcggctga
     2221 ccgtggatcg cccgggcttc gccaacgacg ccgccgaact gctggcgacg ctgccggcgg
     2281 tcgagtcgcg tgcctcgaag accttccccc acgccgaaga cgcgatcgcc ggcttccagc
     2341 cgaacctcaa cttcgcccgc tcctacacga ccgacctgtt caacgcgctg ggcaagctcg
     2401 gcgccgcggc cggctactac gacggcaatg gccactacgt gcgcgcctct ccgtcggcac
     2461 tgaacgtgtt cgaaaactca ggcggcaccc tgactcccgt cgaacggaaa aacgccttgc
     2521 aggcgttcgg cgccgaagcc ggcgtgcgca aaccgtgccc cggcggcgcc acccagaacg
     2581 ccaccgacgg ctccagcccc tacgtaaacc cgccgcagac ggggagcagc gtcagcccgt
     2641 ccgaatgcga cccgagcgac gtgccccccg gcccatgaag cgcgcgatcc cagccctgct
     2701 cctggtcgcg gtggtggtcg tggtcgtgct cctgctcgcc ggcggcgggg gctcgggcaa
     2761 ctcctacgtg gtccgggcga tcttcgacaa cggcgcctac ctggtcaacg gcgagcaggt
     2821 gcgggtggcc ggcgccaacg tcggcgaaat caaggcggtc ggggtgacga tgcccggcga
     2881 cgtcgtcgcc tacaaggacg gcgagccggt cgagaagccg ggcaaagccg tgatcgagct
     2941 ggaaatcacc gacccgggct tccaggattt ccgcgaagac gcgacctgcc agatccggcc
     3001 gcagtcgctg atcggcgaga agttcgtcga ctgccggccg accctgccgc gtgcgccggg
     3061 ctccgagccg ccgccgccgc tgaaagagat ccccgagggc gaggaggggg aaggccagca
     3121 cctgctgccg ctgggcagca acgggaccag cgtcgacccc gacctgatca acaacatcca
     3181 gacgctgccc tactcgcagc gcttccggct gatcttcaac gaactcggcg ccggcttcgc
     3241 cggccgcggc gccgacctcg aagtcctggt caagcgggcc aacccggtgc tgcgcgacgt
     3301 cgacaaactg ttcaagatcc tttccgacca gcgcgaggaa ctggcgcagc tggccagcga
     3361 ctcgaacgag atcctcggcc cgctggcgcg cgagcgccgc cacgtcgccg gcttcatctc
     3421 gaacgccggc gcggccgccg aggcgagctc cgaacggggc gagcagctcg aagaagcgct
     3481 ggacaagttc ccgaccttcc tgcgcgagtt ccgcaccacg atgaccaacc tcgaagggtt
     3541 ctccagcgcg gcgacgccgg tgatggaaga cctcggtgcc gcggccccgt cgctgaccga
     3601 cgcgacccgc accctgaccc cgttctcgga agcgctgacc gtctcgctga agagcctcgg
     3661 cgaaaccggc gaggcgtcgg gcccgatctt cgccgaagcc gacccgatca tccgcaagac
     3721 gaccgaactc tcgaagtcgg gcgtggtgcc gacgcgcgaa ctggcgaaaa tgacgaccag
     3781 cttgagccag tccggcgggt ggaacggcct cgtcgaactg atctacaaca cgtccgcggc
     3841 gatcaacggc ttcgatcagt acggccacta cggccgcacc tacgtaacgc tctcaacctg
     3901 caccaactac atctcgggcg ccagtggtta ctccggaggc ggatgcacct cccgtttcaa
     3961 cgggcccaac gtgggcgaac ctggcgaaga accgctcatg gccctgctga acatgctgaa
     4021 cgagatgaag gtcgacgggt ccgcgggtgg cacgggtgtt ccgctggacc agtccggagg
     4081 ccccgccacc ggcctcggtc aggccgacga aaccgagccc gaaccggaaa tcggtagctc
     4141 ttcgcaggcg gccgccggca ccgaaccgct cctcgactac ctgctggggc catgaggaac
     4201 aaaggcggaa tctcagggct ggcgagcaac ccgatcctgg tcggggcggt gaccgtgctg
     4261 gtcgtgatcg tcgccgtctt cctcgcctac aacgccaaca acggcttgcc gttcgtctcc
     4321 acctacgacc tgaaggcgcg ggtgccgaac gccgacgccc tggtcaaggg caacgaggtg
     4381 cgggtcggcg gcgtgcgg
//
LOCUS       DASRIG010000178         9570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1899290, whole genome shotgun sequence.
ACCESSION   DASRIG010000178 DASRIG010000000
VERSION     DASRIG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899290"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..602
                     /locus_tag="VFB52_04845"
     CDS             186..602
                     /locus_tag="VFB52_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05693.1"
                     /translation="MAGGLGAAAALAIAAGAFAKPDEPKPPPKPKLELRTASQQEALA
                     TRSIKVAVSSKRGRQVRGEATLVVDLFPPHTLKLGPESKQLKRGKAKLLFPLSARKRE
                     VLEFAAQACDQATLAVEARAAGQTGTLTATLHPEGC"
     gene            632..2071
                     /locus_tag="VFB52_04850"
     CDS             632..2071
                     /locus_tag="VFB52_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05694.1"
                     /translation="MNFTEDVLEHFPASAPALLAISRDGGRRVWHFGELVAMSAGLSG
                     ALAARGVAHGDVVMTLVGNRLEWVLAMLACWRMGAVPLPCNAQLRRKDLALRAATAGP
                     ALCVGDEDLLGELPDGIPYMTLAEVGAALDEDRPQEPPAAVADLAPGDPALIAFTSGT
                     TGEPRAALHSQRYLPGQRAQAEQWLGARPGELVWCTTATGWSKSARNVFVAPWLCGAA
                     AMIVDGRFEADERLELIEREGVDVLCQAPTEYRALAKRTALRELPSLRRMVSAGEALN
                     PEVIEAWREATGLAISDGYGQTETGHLTGNLAGDEVRPGSMGRPLPGFDLRIADDELQ
                     VRVATCPTFFDRYLDAEPFDGEWWPTGDVVREDADGYLWYEGRNDDLILSAGYRIGPF
                     EVESALLTHPAVAEAAAVPAPDAERGAVVRAVVVLRDRAPSPELVRELQEHVRSETAP
                     YKYPRIVDFADQLPRTASGKIKRAELRLS"
     gene            2085..3395
                     /locus_tag="VFB52_04855"
     CDS             2085..3395
                     /locus_tag="VFB52_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05695.1"
                     /translation="MPTPRSIQRTYLALMLGNTLAASFIWGINTIFLLDAGLSNAEAF
                     AANAFFTAGMVIFEVPTGVVADTIGRRVSYLLGTLTLTASTALYVLLWQIEAPFWEWA
                     VASMLLGLGFTFFSGAVEAWLVDALAATGFTGDLESVFGKGQIVSGIAMLTGSVAGGL
                     IAQATSLGVPFVVRAGVLAVMFVVAFRLMHDVGFTAEKGRRPVAEMRKITSDSIEYGW
                     RVPAVKWLMLSSLFSGGVGFYAFYALQPYLLELYGDPTAYSVAGLVAAIIAGAQILGG
                     MAAPRIRRLFNRRTSALLGMSTLGVLTLALIGLIEAFAAVIALIVIWGLLFAAEMPIR
                     QAYINGMIPSKQRATILSFDSLMSSAGGVWAQPVLGRSADVWGYAPSYLLSAGIAALA
                     VPFIWLSRREHNPADTLVGAPAEPEAAPDAVEARPALAGAATDC"
     gene            complement(3405..5399)
                     /gene="acs"
                     /locus_tag="VFB52_04860"
     CDS             complement(3405..5399)
                     /gene="acs"
                     /locus_tag="VFB52_04860"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZO05696.1"
                     /translation="MEAATGKAQEALEAELSELLERERFEPPEEFVASALVTDESLHE
                     EAARDPEAWWAKLARDLDWFVEPSEVLDDSNPPFFRWFGDGKLNASHNCLDRHVEAGR
                     GDRVAFHWRGEDGEERDVTYADLLRDTQRLANVLKERGVQAGDVVGIYLPMIPEVAVV
                     MLACARIGAPHNVVFGGFSPDAVKERMQFSEAKALITVDEARRKGKATPIKPAVDEFL
                     ADVPSIETVIVVRNTGADVPMTDGRDVNYDEAIEAADAECPPAELDAEHPLYILYTSG
                     STAKPKGILHTTGGYLTGVAWTHKYVFDLKPDEDVYWCGADVGWVTGHSYIVYGPLLN
                     GATSVMWEGGPDYPDKDIWWEIIERYGVTIFYTAPTAIRACIKWGVEYPARHDLSSLR
                     LLGTVGEPINPKAWLWYWKAIGGERCPIVDTWWQTETGAIMITTLPGAQVAQPGSAGE
                     PLPGIGAAVLTEDGEDAGGEQGLLALTKPWPSMLRTLYKEDERYVETYFSKWGPDTYL
                     VGDAARQDEDGYSWIIGRVDDVINVSGHRLSTAEVESAIVSHPKVAESAVIGQTDEHT
                     GQSICAFVTLEGELEGTEELVGEINEHVAVRIGKFARPKRIVWAHDLPKTRSGKIMRR
                     LLRDIAEGRELGDVTTLRDPDVVEELDEVVKRRASEGEEE"
     gene            complement(5465..7126)
                     /locus_tag="VFB52_04865"
     CDS             complement(5465..7126)
                     /locus_tag="VFB52_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HZO05697.1"
                     /translation="MSFLPPAEINLEAVSIFAVVVAITLGITYWASKRTKDATTFWAA
                     GRGITGVQNGFAIAGDYMSAASFLGIAGLIFLFGFDGFLYSVGFLVAFLIVLYLLAER
                     MRNSGKFTMADVLSFRLNERPARVAAALGTLVVAGFYLIAQMVGAGALIQALVGIDFS
                     LAVLITGGFMLCYVIFGGMLATTWVQIIKAVLLMTATIVLSLFVLGKIGWNPIDIFDR
                     AAEQSDDGGAYLAPGLNFTNPIDTISLGIALLLGTAGLPHILMRFFTVPNAKAARSSV
                     VWAVGLIGAFYVMTTFLGFGSRALLGEAGTTGAVDEAGNLAAPLLAQSVGGGAGSVGG
                     DAFLAIIAAVAFATILAVVAGLVISASGAVAHDVWSNVIRKGRDSEKEEVHVARIAAF
                     AIGAIAIAIAIVGGSDLNVSYMVGLAFAVAASANFPALLLALTWRRFNTTGAVTGVLL
                     GVVSSVGLVLLGPDVRTSDPLIELTQPAIISVPLGFLGCIIGSLLSRDETKAEREFDE
                     LHVRAETGLGAEAPLERPAEAPTPPPRTRRREPVAAMVEHQDRRT"
     gene            complement(7126..7554)
                     /locus_tag="VFB52_04870"
     CDS             complement(7126..7554)
                     /locus_tag="VFB52_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HZO05698.1"
                     /translation="MADTAVGAAVGGSAHHHEIDWGAAARTPEFKELVHRRRSFVVPV
                     LAFVFIWYFGFIALAGYAPDFMGERLIDGFTVGYALALTQFVMTWFLGWLYLRRSNRM
                     FDPLAKRAAERAVELSSTRTAGGNGPRTDGHGNGGREVRH"
     gene            7689..8867
                     /locus_tag="VFB52_04875"
     CDS             7689..8867
                     /locus_tag="VFB52_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05699.1"
                     /translation="MTALATRLTPAHIAFAGIFVVTGLGLLSVGATLPVLPRYVTGPL
                     GGGDLEVGIVTGAFAITGLACRPLAGHLADRRGRRRVVILGSLATVISGLLLFVPAGV
                     PGLIVSRLFLGAGEGAVYTAGAAWIVDLAPPDRRGRIIGLYGLAIWGGLALGPPIGEL
                     ILQAASFEAVWAFATAAPLVGALVALRIPERFRPRTGLDYERSLLAHEALRPGLGLSL
                     AIVGYAAMAAFVVLHLDERAVGHGAAVFAAFAATVVATRVVGGWLPDRYGPIRCAVGA
                     GAVECAGLLTIAASDSLAAALVGAIAMGAGFSLLFPSLALLVINRVPIERRGVAMGTF
                     TAFFDLGMGLGGPLTGAAAAVGGYSAAFVLAAACALGTVTVALSLRRAWAGVPATAPP
                     "
     gene            complement(8824..>9570)
                     /locus_tag="VFB52_04880"
     CDS             complement(8824..>9570)
                     /locus_tag="VFB52_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05700.1"
                     /translation="EDFEREIVVKVNVPEVLRTELRRPSWKRDHVALGTNTDPYQWVE
                     GRYRLTRGVWEAMRDFSNPCSVLTKSPLLLRDLDVFRELVERAEFSANLSIPTLDEKA
                     WRATEPHTPHPRKRIEAVAELVRSGIPTGVLVAPLIPGVNDDPEQVEELLGLLGEAGA
                     ESVSGIGLHLRGEVKEIWFDWLRQYRPDLVPRYEELYRRGAYLPKDEARRLSDLARGP
                     SRARRLRENPAAEPEPATEGQSPAPQPKLF"
BASE COUNT         1335 a         3484 c         3288 g         1463 t
ORIGIN      
        1 cgaggtcgta cgcgagaacc tggacgaatg cgtgctctgc gagctctgcc tcgaggccgc
       61 accccccggg accgtggtcg tcaagaagct ctacgacggc accgagttgc ggcgcggctg
      121 acgccgcggt gccgctcccc gctcatcgct accctggggg gtccatgagg tggggactcg
      181 ggatcttggc cggaggcctg ggtgcggcgg ctgcgctcgc catcgctgcg ggcgctttcg
      241 cgaagccgga tgagccgaag ccgccgccga agccgaagct cgagctgcgg accgcgagcc
      301 agcaggaggc gctcgcgacg cggtcgatca aggtcgcggt gagctccaag cgcggcagac
      361 aggtgcgcgg cgaggcgacg ctggtggtcg acctgttccc ccctcacacg ctcaagctcg
      421 gcccggagtc caagcaactc aagcgcggaa aggcgaagct cctcttcccg ctcagcgccc
      481 gcaagcgcga ggtgctcgag ttcgcggccc aggcctgcga ccaggcgacg ttggccgtcg
      541 aggcccgcgc cgcgggacag acgggcacgc tcaccgcgac cctgcacccg gagggctgct
      601 agccgagcct cgtgcggaac accgccgttc agtgaacttc accgaggacg tcctcgagca
      661 cttcccggcg tcggcgcccg ctctgctcgc gatctcgcgc gacggcgggc ggcgcgtctg
      721 gcacttcggc gagctggtgg cgatgtcggc gggcctgtcg ggtgccctcg cagcccgcgg
      781 cgtcgcccac ggcgacgttg tcatgaccct cgtcggcaac cgcctggagt gggtgctggc
      841 gatgctcgcc tgttggcgga tgggcgcggt accgctgccc tgcaacgccc agctgcgccg
      901 caaggacctc gcgctgcgcg ccgcgaccgc cggcccggca ctttgtgtcg gcgacgagga
      961 cctgctcggc gaactgccgg acgggatccc gtacatgacc ctggccgagg tcggcgccgc
     1021 cttggacgag gatcgcccac aggagccgcc ggccgccgtc gccgacctcg cccccggcga
     1081 cccggcgctg atcgccttca cgtccgggac gacgggcgag ccccgcgcgg ccctgcactc
     1141 ccagcgctat ctgcccggac agcgcgcgca ggccgagcaa tggctcggag cccgtcccgg
     1201 tgagctggtc tggtgcacaa ccgccacggg ttggtcgaag tcggcgcgga acgtcttcgt
     1261 cgcgccgtgg ctgtgcggcg cggcggcgat gatcgtcgac ggccgcttcg aggccgacga
     1321 gcggctggag ctcatcgagc gcgagggcgt ggacgtgctc tgccaggccc cgaccgagta
     1381 ccgggcgctg gccaagcgca cggcactgcg agagctgccc tcgctgcggc ggatggtctc
     1441 agcgggcgag gcgctcaacc cggaggtcat cgaggcgtgg cgcgaggcga ccgggctcgc
     1501 gatcagcgac ggctacggac agacggagac gggccatctg accggcaacc tcgccggcga
     1561 tgaggtccgc ccgggctcga tgggccggcc gctgcccggg ttcgacctgc ggatcgccga
     1621 cgacgagctc caggtccggg tcgcgacctg cccgacgttc ttcgaccggt acctggacgc
     1681 agagccgttc gacggcgagt ggtggccgac cggcgacgtg gtccgcgagg acgccgacgg
     1741 ctacctctgg tacgagggcc gcaacgacga cctgatcctc tcggccggat accggatcgg
     1801 cccgttcgag gtcgagtcgg cgctgctcac ccaccccgcg gtcgccgagg ccgccgccgt
     1861 cccagcgccc gatgccgagc ggggcgcggt cgtgcgcgcg gtcgtcgtcc tgcgcgaccg
     1921 tgcgccgtcg ccggagctgg tccgcgagct gcaggagcac gtcagatccg agaccgcgcc
     1981 ctacaagtac ccgcggatcg tcgacttcgc cgaccagctc ccgaggaccg ccagcgggaa
     2041 gatcaagcgc gccgagctcc gcctctcgta acgtcaaggg cgagatgccg accccgcggt
     2101 cgatccagcg gacctacctg gcgctgatgc tgggcaacac gctggcggcc tcgttcatct
     2161 ggggcatcaa cacgatcttc ctcctcgacg cggggctctc gaacgccgag gcgttcgcgg
     2221 ccaacgcgtt cttcaccgcg ggcatggtga tcttcgaggt cccgaccggg gtggtcgccg
     2281 acacgatcgg ccgccgcgtc tcctacctgc tgggcacgct gacgctgacc gcgtcaaccg
     2341 ccctgtacgt gctcctctgg cagatcgagg cgccgttctg ggagtgggcc gtcgcgtcga
     2401 tgctgctcgg gctcggcttc accttcttct ccggcgcagt cgaggcgtgg ctggtcgacg
     2461 cgctcgcggc gaccggcttc accggggatc tcgagtcggt cttcgggaag ggacagatcg
     2521 tctcggggat cgcaatgctg accgggtcgg tcgccggcgg cctgatcgcc caggcgacca
     2581 gcctcggggt gccgttcgtg gtccgggccg gcgtcctcgc ggtgatgttc gtcgtggcct
     2641 tccggctcat gcacgatgtc ggcttcaccg ccgagaaggg ccggcggccg gtcgccgaga
     2701 tgcgcaagat cacgtccgac tcgatcgagt acggctggcg ggtcccggcg gtcaagtggc
     2761 tgatgttgag ctcgctgttc agcggcggcg tcggcttcta cgcgttctac gccctgcagc
     2821 cgtacctgct ggagctctac ggcgacccga ccgcgtactc ggtcgccggc ttggtggcgg
     2881 cgatcatcgc cggcgcccag atcctcggcg ggatggcggc gccccgcatc cggcggctgt
     2941 ttaaccgccg gacctccgcg ctgctcggga tgtcgacgct cggcgtcctc accctggcgc
     3001 tcatcgggct gatcgaggcc ttcgccgcgg tgatcgcgct gatcgtgatc tgggggctct
     3061 tgttcgccgc cgagatgccg atccgccagg cctacatcaa cgggatgatc ccgtcgaagc
     3121 agcgcgcgac gatcctctcg ttcgactcgc tgatgtcctc cgccggcggc gtctgggctc
     3181 agccggtcct cgggcgctcg gcggacgtct ggggctacgc gccgtcctat ctgctcagcg
     3241 ccgggatcgc ggcgctcgct gtcccgttca tctggctctc gcgtcgcgag cacaacccgg
     3301 ccgacacgct cgtcggcgcg ccggcggagc cggaggccgc gccggacgcg gtcgaggcgc
     3361 gcccggcgct cgcgggcgcg gcgaccgact gctgacgctg ggccctactc ctcctcgccc
     3421 tcgctcgcgc gccgcttgac gacctcgtcg agctcctcga ccacgtcggg gtcgcgcagc
     3481 gtggtcacgt cgccgagctc gcggccctcg gcgatgtcgc gcagcagccg gcgcatgatc
     3541 ttccccgagc gggtcttcgg caggtcgtgg gcccagacga tccgcttcgg gcgcgcgaac
     3601 ttgccgatcc gcacagcgac gtgctcgttg atctccccca ccagctcctc ggtcccctcg
     3661 agctcgccct cgagggtgac gaaggcgcag atcgactggc ccgtgtgctc gtcggtctgt
     3721 ccgatcaccg cggactccgc gaccttcgga tgcgagacga tggccgactc gacctcggcg
     3781 gtcgacagcc ggtgcccgga gacgttgatc acgtcgtcga cccggccgat gatccaggag
     3841 tagccgtcct catcctggcg cgcggcgtcg cccaccaggt acgtgtccgg gccccacttc
     3901 gagaagtagg tctcgacgta gcgctcgtct tccttgtaga gcgtgcgcag catcgacggc
     3961 cacggcttgg tcagcgccag cagtccctgc tccccgccgg cgtcctcgcc gtcctcggtc
     4021 agcacggcgg ccccgatccc cggcaggggc tcccccgccg agcccggctg cgccacttgg
     4081 gcgccgggca gagtcgtgat catgatcgcg ccggtctcgg tctgccacca cgtgtcgacg
     4141 atcggacagc gctcgccgcc gatcgccttc cagtaccaga gccacgcctt cgggttgatc
     4201 ggctccccga ccgtgcccag caggcgcaac gaggacaggt cgtgcctcgc cgggtactcg
     4261 acgccccact tgatgcaggc gcggatcgcg gttggcgccg tgtagaagat cgtcacgccg
     4321 taccgctcga tgatctccca ccagatgtcc ttgtcgggat agtcgggccc gccctcccac
     4381 atcaccgacg tcgcgccgtt gagcaggggg ccgtagacga tgtacgagtg cccggtcacc
     4441 cagcccacgt cggcgccaca ccagtagacg tcctcgtcgg gcttcaggtc gaagacgtac
     4501 ttgtgggtcc acgcgacccc ggtcagataa ccgcccgtgg tgtgcaggat ccccttcggc
     4561 ttcgcggtcg aaccggaggt gtagaggatg tagagcgggt gctcggcgtc gagctccgcc
     4621 ggcgggcact ccgcgtcggc ggcctcgatc gcctcgtcgt agttgacgtc gcgaccatcg
     4681 gtcatcggca catcggcgcc ggtgttgcga acgacgatca cggtctcgat cgatggcacg
     4741 tcggccagga actcgtcgac ggccggcttg atcggtgtcg ccttgccctt gcgccgtgcc
     4801 tcgtcgacgg tgatcagcgc cttcgcctcg gagaactgca tccgctcctt gaccgcgtca
     4861 ggcgagaagc cgccgaagac gacgttgtgc ggagcgccga tgcgcgcgca ggcgagcatg
     4921 accaccgcga cctcggggat catcggcagg tagatgccga ccacgtcgcc ggcctgcacc
     4981 ccacgctcct tgagcacgtt ggcgagccgc tgggtgtcgc ggagaaggtc cgcgtaggtg
     5041 acgtcccgct cctcgccgtc ctcgccgcgc cagtggaacg ccacccgatc gccgcgcccg
     5101 gcctccacgt gacggtcgag gcagttgtgc gaggcgttca gcttgccgtc gccgaaccag
     5161 cgaaagaacg gcgggttgga gtcgtcgagg acctcgctcg gctccacgaa ccagtcgagg
     5221 tcgcgggcca gcttcgccca ccacgcctcg ggatcgcgcg cggcctcctc gtgcaacgac
     5281 tcgtcggtga cgagggcaga ggcgacgaac tcctcgggcg gctcgaagcg ctcgcgctcg
     5341 agcagctcgc tgagctccgc ctcgagcgcc tcctgcgcct tgccggttgc ggcctccatc
     5401 agcctcctcg catcgtcgaa accctgggcc gcgggccaga gcgaaggccc gcggcccgat
     5461 tgtcctacgt ccggcggtct tggtgttcga ccatcgcggc caccggctcg cggcgacgcg
     5521 tccgcggcgg cggcgttggc gcctccgccg gccgctcgag cggcgcctcg gcgccgagtc
     5581 cggtctccgc gcgcacgtgg agctcgtcga actcacgctc ggccttcgtc tcgtcacgcg
     5641 agagcaggct tccgatgatg cagcccagga acccgagcgg cacggagatg atcgccggct
     5701 gggtgagctc gatcagcggg tcggacgtgc gaacgtcggg cccgagcagc acgagcccga
     5761 ccgagctgac cactcccaac agcacgccgg tgaccgcgcc tgtggtgttg aaccgtctcc
     5821 aggtcaacgc cagcaacagc gccgggaagt tggcgcttgc ggcaaccgcg aacgcgaggc
     5881 cgaccatgta ggagacgttg aggtcggagc cgccgacgat cgcgatcgcg atcgcgatcg
     5941 cgccgatcgc aaacgccgcg atacgggcga cgtggacctc ctccttctcc gagtcacggc
     6001 ccttgcggat cacgttcgac cagacgtcgt gagcgaccgc tccggacgcc gagatcacga
     6061 gccccgcgac caccgcgagg atggtcgcga aggcaaccgc ggcgatgatc gccaggaacg
     6121 cgtcgccgcc gaccgagccg gctccaccgc cgacgctctg cgccagcaac ggggcggcga
     6181 gattcccggc ctcgtcgacc gctcccgtcg tgcccgcctc gccgagcagt gcccgagagc
     6241 cgaagcccag gaacgtcgtc atcacgtaga aggcgccgat caggcccacc gcccagacga
     6301 ccgacgagcg cgccgccttg gcgttcggca cggtgaagaa ccgcatcagg atgtgcggca
     6361 gcccggcggt cccgagcagc agcgcgatcc cgagcgagat cgtgtcgatc gggttggtga
     6421 agttgagccc cggggcgaga taggccccgc cgtcgtcgga ctgctccgcc gcccggtcga
     6481 agatgtcgat cgggttccag ccgatcttgc ccagcacgaa cagcgacagc acgatcgtcg
     6541 ccgtcatcag cagcaccgcc ttgatgatct gcacccaggt ggtcgccagc atgccgccga
     6601 agatcacgta gcagagcatg aacccgcccg tgatgagcac agccagactg aagtcgatcc
     6661 cgaccagggc ctggatcaac gcgccggcgc cgaccatctg cgcgatcaaa tagaagcccg
     6721 cgacgaccag ggtcccgagc gcagcggcga cccgggcggg gcgctcgttg agccggaacg
     6781 agaggacgtc ggccatcgtg aacttgcccg agttgcgcat ccgctccgcc agcaggtaga
     6841 gcacgatcag gaacgcgacc agaaagccga ccgagtacag gaatccgtcg aagccgaaca
     6901 ggaagatcag cccggcgatg ccgaggaacg aggccgccga catgtagtcg ccggcgatcg
     6961 cgaacccgtt ctgcaccccg gtgatccccc gaccggcggc ccagaacgtg gtcgcgtcct
     7021 tggtccgctt cgaggcccag taggtgatcc cgagggtgat cgcgacgacc accgcgaaga
     7081 tcgagaccgc ctccaaattg atctctgcgg gtgggaggaa gctcatcagt gcctcacttc
     7141 ccggccgccg ttaccgtggc catcggtgcg agggccattg ccgccggccg tcctggtcga
     7201 cgacagttcc accgcccgct cagcagcccg cttcgcgagc gggtcgaaca tccggttgga
     7261 gcgccgcagg tacagccagc ccaggaacca ggtcatcacg aactgggtca gcgccagcgc
     7321 gtagccgacg gtgaagccgt cgatcaagcg ctcgcccatg aagtcggggg cgtagcccgc
     7381 gagggcgata aagccgaagt accagatgaa tacgaaagcc agcaccggga ccacgaacga
     7441 gcggcgcctg tgcaccagct ccttgaactc aggggtgcgc gcggcggcgc cccagtcgat
     7501 ctcgtgatgg tgcgcggatc cccccacagc cgcccccaca gctgtgtcag ccatctctgc
     7561 tcccttgtcg gggttctcac ccggggagcc ccccggtctc ccgggtcctc tcctgccacc
     7621 caacttccca aaggctcgca gcgcaaacca aagtcgccga aatcgtcgtt ccagctattg
     7681 attcaggcat gacggcgctc gcgacccgcc tcacccccgc ccacatcgcg ttcgctggga
     7741 tcttcgtcgt gaccggcctc ggcctgctct cggtcggcgc aacgctgccc gtgcttcctc
     7801 gctacgtcac cggcccgctc ggcggcggcg acctcgaggt cgggatcgtc acgggcgcgt
     7861 ttgcgatcac cggcctcgcc tgccgcccgc tggccgggca cctcgccgac cggcgcggcc
     7921 ggcgccgcgt ggtcatcctc ggctcgctgg cgaccgtgat ctccgggttg ctgctgttcg
     7981 tcccggcggg ggtccccggc ctgatcgtct cgcggctgtt cctgggcgcc ggcgagggcg
     8041 cggtctacac ggccggcgcg gcctggatcg tcgatctggc gccgcccgac cgccgcggcc
     8101 gcatcatcgg cctctacggg ctcgcgatct ggggcgggct ggccctcggg ccgccgatcg
     8161 gtgagctgat cctgcaggcc gcgagcttcg aggcggtctg ggcgttcgcc accgcggcgc
     8221 cgctcgtcgg ggccctcgtg gcgttgcgaa tccccgagcg cttccgcccc agaacgggcc
     8281 tcgactacga gcgctcgctg ctcgcccacg aggcgctgcg cccgggcctc gggctatcgc
     8341 tcgcgatcgt cggctacgcc gcgatggccg cgttcgtcgt cctgcacctc gacgagcgcg
     8401 cggtgggtca cggtgcggcg gtgttcgcag cgttcgccgc caccgtggtc gcgacgcggg
     8461 tcgtcggcgg ctggctgccc gaccgctacg gcccgatccg gtgcgccgtc ggcgccggtg
     8521 cggtcgaatg cgccgggctc ctcacgatcg ccgcctccga cagcctggcg gcggcgctgg
     8581 tgggcgcgat cgcgatgggg gccggcttct cgctgctctt cccgtcgctg gcgctgctcg
     8641 tcatcaaccg ggtcccgatc gagcgccgcg gcgtcgccat gggcacgttc accgcgttct
     8701 tcgacctcgg catggggctc ggcggcccgc tgaccggggc cgctgccgcc gtcggcggct
     8761 actcggcggc gttcgtgctc gccgcggcat gcgccctcgg gaccgtcacc gtcgcgcttt
     8821 cgctcagaag agcttgggct ggggtgccgg cgactgcccc tccgtagcgg gctctggctc
     8881 ggccgcaggg ttctcgcgca gccggcgcgc ccgcgagggc ccgcgcgcga gatccgagag
     8941 ccgccgggcc tcgtccttgg gcaggtaggc gccgcggcgg tacagctcct cgtagcgagg
     9001 cacgaggtcg ggccggtact ggcgcagcca gtcgaaccag atctccttga cctctccgcg
     9061 cagatgcagg ccgatgccgc tcacgctctc ggcgcccgcc tcacccaaca gcccgagcag
     9121 ctcctcgacc tgctcagggt cgtcgttgac gccggggatc agcggggcca ccagcacccc
     9181 ggtcgggatc ccgctgcgga ccagctccgc caccgcctcg atccgcttgc gcggatgggg
     9241 cgtgtgcggc tcggtcgccc gccacgcttt ctcgtcgagc gtcgggatcg acaggttcgc
     9301 gctgaattcg gcccgttcga ccagctcgcg gaacacgtcg agatcgcgaa gcaggagcgg
     9361 cgacttggtc aacaccgagc acgggttgga gaagtcgcgc atcgcctccc agacgccgcg
     9421 ggtgagccga tagcggccct cgacccactg gtacgggtcc gtgttggtgc cgagcgcgac
     9481 gtgatcgcgc ttccacgacg gccgccgcag ctcggtgcgc agcacctcgg gcacgttgac
     9541 cttgaccacg atctcgcgct cgaagtcctc
//
LOCUS       DASRIG010000179         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1142133, whole genome shotgun sequence.
ACCESSION   DASRIG010000179 DASRIG010000000
VERSION     DASRIG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142133"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFB52_04885"
     CDS             <1..345
                     /locus_tag="VFB52_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZO05701.1"
                     /translation="LKRISIDLYQHAADHARERGIILADTKFEFGKHAGAEIVLGDEV
                     LTPDSSRFWPADSYQPGATPPSFDKQFVRDWASAQGWDKSPPAPELPADVVEQTRGKY
                     VEAFERITETSF"
     gene            342..563
                     /gene="purS"
                     /locus_tag="VFB52_04890"
     CDS             342..563
                     /gene="purS"
                     /locus_tag="VFB52_04890"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZO05702.1"
                     /translation="MKARVLIRPKEGILDPQGKAVERALPALGFEGVEHVRVGRMVEL
                     EADDPARLAELCEKLLANPLIEDYEIQTL"
     gene            560..1228
                     /gene="purQ"
                     /locus_tag="VFB52_04895"
     CDS             560..1228
                     /gene="purQ"
                     /locus_tag="VFB52_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZO05703.1"
                     /translation="MIWGVVQFPGSCDERDALLACTIASGEARMIWHEDDDLGGVDGV
                     VVPGGFSYGDYLRAGAIARFSPAMEAVAEHAAAGKPVLGICNGFQVLCEAGLLPGALL
                     RNESMRFVCRQLELIELPVGAFAVRRDQLTLNRLSIPAKHGEGRYWAPEETLDALEAN
                     DQVAYRYAHGQNPNGSARDIAGITNEAGNVLGLMPHPEHAVDPLTGSTDGLRVFQRLE
                     GVLR"
     gene            1225..3414
                     /gene="purL"
                     /locus_tag="VFB52_04900"
     CDS             1225..3414
                     /gene="purL"
                     /locus_tag="VFB52_04900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZO05704.1"
                     /translation="MSTATPKYRELGLTDHEYGLIVEKLGREPGDVELAMFSLLWSEH
                     CAYKHSRKLLGKLPTEGERVVMGPGENAGAVDVGNGYAVAFKVESHNHPSAVEPFEGA
                     ATGVGGIMRDVFAIGARPIALLNSLRFGELDSARSRHLLDGAVRGIGHYGNSIGVATV
                     GGEVYFEEPYEHNCLVNAMCIGIAPTAEMVRAAAAGVGNAVVLMGASTGRDGIGGASV
                     LASAELGEADEAKRPTVQIGDPFEESKVLECCLELLAKGLLVSLQDLGAAGLTSSAGE
                     MASAGGVGIDIDVAKVPLREADMEPFEIMVSESQERMLAVVVPEKVAEVRAICEKWQT
                     GSAEIGVVTDSGQIRILRGADVVGEMPVEALVDGCPLYDLEPAEPETWIYGNRKTLEP
                     GIAPEDELLALLAAPSIASKRWVFEQYDSIVGSRTVRRPESADAAVLALPEADGAIAV
                     SIDGNGRRVACDPYAGTVEAVLECAQNLACVGAEPLGLTNCLNFGNPEKPTPAWQLDR
                     AVSGLADACTALGVPVVGGNVSLYNEGPDGPIYPTPVVAMVGELPDPHEVGGSGFAGE
                     EDAIGLLGPFAPTLAGSELAKQRGELAMGLPQPDLEAVAAACATVREAVRRGEVSSAH
                     DISDGGLACALAEAAIGGGFGCRVDLQPLRERGCSPEEALFGEGSGGFLLSGNRAALV
                     ELGAIPIGEVGGAKIEIAAGDRSLIVGLADAEGAWRSLGARAEAGGL"
     gene            3500..>4379
                     /locus_tag="VFB52_04905"
     CDS             3500..>4379
                     /locus_tag="VFB52_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05705.1"
                     /translation="MLVGALAGLICVLALPPAGAAAASRVGNECTADKGTTLGGIVQL
                     GQSAGPIPMVAPASGVITAWSATVGAALPDAPHRIHVLRAAGPTSFVLLASSPQVSLR
                     PGPNSFPVRLPIAAGDRIGLEGTEQSSTYFCATPSSFDRIGVLSFGLMPGAPQPLGLT
                     DDQVAVPVVAVIEPDADGDGYGDETQDRCPGSAAHQDCPAAIVDATADAGRATVTVSV
                     TTDRPATVRVSGNVRLGKGAKAKLAARAKTVAPGKLTRFTLKFPRKLKSKLKELESGR
                     RLTLKITASATNATGQV"
BASE COUNT          652 a         1582 c         1543 g          602 t
ORIGIN      
        1 ctgaagcgga tctcgatcga cctctaccag catgccgccg accacgcgcg cgagcgcggg
       61 ataatcctcg ccgacaccaa gttcgagttc ggcaagcacg cgggcgccga gatcgtgctc
      121 ggcgacgagg tgttgacccc ggactcctcg cgcttctggc cggccgacag ctaccagccg
      181 ggcgccacgc ccccctcctt cgacaagcag ttcgtgcgcg actgggcgag cgcccagggc
      241 tgggacaaat cgccgccggc gccggagctg ccggccgacg tggtcgagca gacgcgcggc
      301 aaatacgtcg aggctttcga acggatcacc gagacgagct tttgaaagcc cgcgtcctga
      361 tccggcccaa ggaggggatc ctcgacccac agggcaaggc ggtcgagcgg gcgctgccgg
      421 cgctcggttt cgagggcgtc gagcacgtac gggtcgggcg catggtcgag ctcgaggccg
      481 atgatcccgc gcggctggcg gagctctgcg agaagctcct cgccaacccc ctgatcgagg
      541 actacgagat ccagacgctg tgatctgggg cgtcgtccag ttccccgggt cctgtgacga
      601 gcgcgatgcg ctgctcgcct gcacgatcgc cagcggcgag gcgcggatga tctggcacga
      661 ggacgacgac ctcggtggcg tcgacggcgt cgtcgttccc ggcggcttct cctacggcga
      721 ctacctgcgg gcgggcgcga tcgcccgctt ctcgccagcg atggaagcgg tcgccgagca
      781 cgccgccgcc ggcaagccgg tgctgggaat ctgcaatggc ttccaggtcc tctgcgaggc
      841 cggccttctg cccggtgccc tgctgcgcaa cgagagcatg cgctttgtct gccgtcagct
      901 ggaactgatc gagctgccgg tcggtgcctt cgcggtgcgc cgggaccagc tgaccctgaa
      961 ccgcctctcg attccggcca agcacggcga agggcgctac tgggcgccgg aggagacgct
     1021 cgacgcgctc gaagccaacg accaggtcgc ctaccgttac gcgcacggcc agaatcccaa
     1081 cggctcggcc cgcgacatcg ccggcatcac caacgaggcc ggcaacgtgc tcggcctgat
     1141 gccacacccc gagcacgccg tcgacccgct gacaggttcg accgacgggc tgcgggtctt
     1201 tcagcggctc gagggagtgc tgcggtgagc acggcgaccc cgaaataccg cgagctcggg
     1261 ctgaccgacc acgagtacgg gctgatcgtc gagaagcttg gtcgcgagcc gggcgacgtc
     1321 gagctggcga tgttcagcct gctctggtcc gagcactgcg cctacaagca ctcgcgcaaa
     1381 ctgctcggca agctgccaac cgagggcgag cgggtggtga tgggaccggg cgagaacgcc
     1441 ggggcggtcg acgtcggcaa cggctacgcg gtcgccttca aggtcgagtc ccacaaccac
     1501 ccgagcgcgg tcgaaccgtt cgagggcgcg gccaccgggg tcggcgggat catgcgcgac
     1561 gtcttcgcga tcggcgcccg cccgatcgcg ctgctcaact cgctgcgctt cggcgagctc
     1621 gactcggccc gctcgcgcca cctgctcgac ggcgccgtgc gcgggatcgg ccactacggc
     1681 aactcgatcg gggtggcgac ggtcggcggc gaggtctact tcgaggagcc ctacgagcac
     1741 aactgcctgg tcaacgcgat gtgcatcggg atcgccccga ccgccgagat ggtccgcgcc
     1801 gccgccgccg gggtcggcaa cgcggtcgtg ctgatgggcg cctcgaccgg gcgcgacggg
     1861 atcggcggcg cctccgtgct cgcctcggcc gagctcggcg aggccgacga agcgaagcgg
     1921 ccgacggtcc agatcggcga cccgttcgag gagtcgaagg tgctggagtg ctgcctcgag
     1981 ctgctggcga agggtctgct ggtctcgctg caggacctcg gcgccgccgg gctcacctcc
     2041 tcggccggcg agatggcctc ggccggcggg gtcgggatcg acatcgacgt cgccaaggtg
     2101 ccgctgcgcg aggccgacat ggagccgttc gagatcatgg tctccgagtc gcaggagcgg
     2161 atgctggcgg tcgtcgtccc cgagaaggtc gccgaggtaa gggcgatctg cgagaaatgg
     2221 cagaccggct cggcggagat cggcgtggtc accgactccg ggcagatccg catcctgcgc
     2281 ggcgccgacg tggtcggcga gatgccggtc gaggcgctgg tcgacggctg tccgctctac
     2341 gacctcgagc cggccgaacc ggagacttgg atctacggga accgcaagac cctcgagccc
     2401 gggatcgctc ccgaagacga gctgctcgcc ctgctcgcgg cgccgagcat cgcctccaag
     2461 cgctgggtct tcgagcagta cgactcgatc gtcggctcgc gcacggtgcg ccggccggag
     2521 agtgccgacg ctgccgtgct ggcgctgccg gaggccgacg gcgcgatcgc cgtctcgatc
     2581 gacggcaacg gccgccgggt cgcctgcgac ccctacgccg gcacggtcga ggcggtgctc
     2641 gagtgcgccc agaacctcgc ctgcgtcggc gccgagccgc tcggcctcac caactgcctc
     2701 aacttcggca accccgagaa gccaaccccg gcctggcaac tcgaccgcgc ggtcagcggc
     2761 ctcgccgacg cctgcacggc gctcggcgtg ccggtcgtcg gtggcaacgt ctcgctctac
     2821 aacgagggtc ccgacggccc gatctacccg accccggtgg tcgcgatggt cggcgagctg
     2881 cccgacccgc acgaggtcgg cggcagcggc ttcgccggcg aggaagacgc gatcggcctg
     2941 ctcggcccgt tcgctcccac cctggccggc tcggagctgg ccaagcagcg cggcgagctg
     3001 gcgatgggcc tgccccagcc tgacctcgag gcggtcgccg ccgcctgcgc gaccgtccgc
     3061 gaagcagtcc gccgcggcga ggtctcctcc gcccacgaca tcagcgacgg cggcctcgcc
     3121 tgcgcgctcg ccgaggcggc gatcggcggc ggcttcggct gccgggtcga cctgcaaccg
     3181 ctgcgcgaac gtggctgctc gccggaggag gcgctgttcg gcgagggcag cggcggcttc
     3241 ctgctgagcg gcaaccgcgc cgccctggtc gagctcggcg cgatcccgat cggcgaggtc
     3301 ggcggcgcca agatcgagat cgccgccggc gaccgcagcc tgatcgtcgg cctcgccgac
     3361 gccgagggcg cctggcgctc gctcggcgct cgcgcggagg cgggcgggct ctagacagat
     3421 cttcgtccag ctgtcgcagg ccacgactag gctgtgctgc gccgtgtcac agccgtccac
     3481 tgggagaaac gtttcgcgct tgctggtcgg ggcgctcgcc gggctgatct gcgtgctcgc
     3541 cctgccgccg gccggcgccg ctgcggcctc ccgggtcggc aacgaatgca cggccgacaa
     3601 aggcacgacc cttggcggga ttgtccagct gggtcagagc gccggcccga tcccgatggt
     3661 cgcaccggcc tcgggcgtca tcaccgcctg gagcgccacg gtcggagccg ccctccccga
     3721 cgcgccgcac cggatccatg tcctccgtgc tgccgggccg acgagcttcg tcttgctcgc
     3781 gtccagcccc caggtctcgc tccgccccgg tcccaactcg ttcccggtca ggctcccgat
     3841 cgcggccggc gaccggatcg ggctggaagg caccgaacag tccagcacct acttctgcgc
     3901 cacgccaagc agcttcgacc ggatcggcgt gctgtcgttc ggcctcatgc ccggagcccc
     3961 ccagccgctc ggcctcaccg acgaccaggt cgcggtgccc gtcgtcgccg tgatcgagcc
     4021 cgacgcggat ggcgatggct acggcgacga gacccaggac cgctgcccag gcagcgccgc
     4081 ccaccaagac tgcccggccg cgatcgtcga cgcgaccgcg gacgcaggcc gcgccacggt
     4141 gacggtcagc gtcaccaccg accgccctgc gacggtgcgg gtgagcggaa atgtaaggct
     4201 cggcaaaggc gccaaagcga aacttgccgc ccgcgcgaag acggtcgcgc cgggcaagct
     4261 gacgcgcttc acgctcaaat ttccccgcaa gctcaagagc aagctgaagg agctggagtc
     4321 gggcaggagg ctgacgctga agatcaccgc cagcgcgacc aacgccaccg gccaggtca
//
LOCUS       DASRIG010000180         5760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1899884, whole genome shotgun sequence.
ACCESSION   DASRIG010000180 DASRIG010000000
VERSION     DASRIG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899884"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..676
                     /locus_tag="VFB52_04910"
     CDS             134..676
                     /locus_tag="VFB52_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HZO05706.1"
                     /translation="MAAALVAPTWASALARRRELAEGDAWIAALALPILVAHQTEEWV
                     RPGGFLPFCNERLLGSRAPDWPLTERLGFHVNVTLGWGSAVAATALWRRSAAPAAFVL
                     WIEAGNALMHGGMALRERRYNPGLATALGLMAPHAALGTVRLRRSGRLSRRAAVLAAA
                     AGIGFSAALPVAMKARLRRG"
     gene            complement(627..986)
                     /locus_tag="VFB52_04915"
     CDS             complement(627..986)
                     /locus_tag="VFB52_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05707.1"
                     /translation="MSGIDTGILWRAALVQLVAVAVLSIALALALPKSFFEDYGWISG
                     PLAWLLCAVLTARVLRLPPAPTLLGAVLAGLPSLLFVVIGLHWAGAVVAVGFFAVWCA
                     RLSPAAGAPSSPPAGQR"
     gene            complement(999..1319)
                     /locus_tag="VFB52_04920"
     CDS             complement(999..1319)
                     /locus_tag="VFB52_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05708.1"
                     /translation="MPELSHHHRATVEKILAHPASGNVEWRAVLSLLEAVGEVSEEHD
                     GHVKVTVGPETETLHRPREKDVDEQMIVNLRRLLVQAGYGGDGAVEDSRDRNYGDNRW
                     GKPE"
     gene            complement(1353..1679)
                     /locus_tag="VFB52_04925"
     CDS             complement(1353..1679)
                     /locus_tag="VFB52_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZO05709.1"
                     /translation="MKKIALLTLVLVSLLSIQAAGVAQGDGSKKVGIVNFAFKPGTLT
                     VGKGTKVEFENESNTAHTATRNGGGFNSGRIRPGNQFSVRFNRTGTFRYHCTIHPEMR
                     GKIVVQ"
     gene            complement(1712..2062)
                     /locus_tag="VFB52_04930"
     CDS             complement(1712..2062)
                     /locus_tag="VFB52_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05710.1"
                     /translation="MIPPRMISLQRNHFIALIVGVCILSAGIGTAVALIAQTGPEGPA
                     GKQGKAGPQGPKGPRGPSGEAAVEGVQAEVEELRGQVEGSAELEERVQQLEDEVSNIG
                     GFEEELCEEDDFFC"
     gene            2085..2157
                     /locus_tag="VFB52_04935"
     tRNA            2085..2157
                     /locus_tag="VFB52_04935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2118..2120,aa:Val,seq:tac)
     gene            complement(2158..3786)
                     /locus_tag="VFB52_04940"
     CDS             complement(2158..3786)
                     /locus_tag="VFB52_04940"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HZO05711.1"
                     /translation="MADPRLIAAALATLALLVFAPAASAALSAETLYEPDTVVVIDLK
                     LSEAAVAALEAEPDEYVEGEFRIAETDGTPEGIGPYTEPLTVGVRLKGGAGSFKGLDK
                     KAAFKVKFDEFVDDQTFLGLEKLTLNNMAQDPSMVHEALVYPAFRAAGVPAPHTGYAY
                     VYLNGIDYGLHLNLEVWDKIALEKRFGAFQNPPQHLYEGGAGQDLEPGMAAEYEVDEG
                     KSKTRTDLEALITAANATEPSFTPRLAPVADLEEMVAMWAAERYLGQWDGYSGGLPNN
                     YYLYSDPAGVFQMLPSGTDQTLSDWWYSFTSKGGLLFNQCLAEEACYAAYVKALETVE
                     ETSVALDLPDQAKALAALLAPWQEQEVEESERAPFDLEFIEEEFEKTLKFLAKRPQVL
                     ADWLGTGELPEEPNEEEPEEEEEPKEDPGKPNPFEEIPKVPQPFTIDSRLELDRSRLG
                     RGLVIVRATVSGPGALSLIGSTGPADDRRRACTVAAKQVQAGPAILHCRLSEATRRRL
                     AKAPLTLRLNVNFQPGDGNPLLTVSRLARLPRQA"
     gene            complement(3768..5375)
                     /locus_tag="VFB52_04945"
     CDS             complement(3768..5375)
                     /locus_tag="VFB52_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05712.1"
                     /translation="MIDLENRDLGLLGVARIGISFANWSFAIALGVYGFEAHGVVGVG
                     LVALIRLLPGAIVSPFCGLLADRYSRRNLLVRASAVMAVVLAGAAFAASQDASTDVVF
                     IFPALYAIALATYMPAEAALFPVYARTPQELSASNVNHVAMENAGFIVAAIATGFLLT
                     ATSPGTVFAVAAAVTVVVALLLLAVKRDSRPEYEDEEDVAGILREASRGARALRDNPG
                     LRLASLTLVVLLAFEGLADVILVTLALHLLHLEEGSVGFLNAAWGIGALVGGASLALL
                     LDRGRLVVAIAGGSLVIGVATLIPGIWPEQVTSYIGWFGIGIGFTYVEVAAKTLMHRL
                     GDDETMGRLTGALESGRLGAQALGSILAIALVELLHPDGALIVVGLLLPVFVFICWTR
                     LRSYEIGAPVAEEQYQLLRGNSIFAPLPIATVERLSHDLVAVDAPAGQDVIVQGDIGD
                     RFYLIEAGQVEVFEHGEFRRNEGPGESFGEIALLHDVPRTATVRTTVDTRLLALDREQ
                     FLLAVTGHRRSQQRAQGVSEERWLIRD"
     gene            5382..>5760
                     /locus_tag="VFB52_04950"
     CDS             5382..>5760
                     /locus_tag="VFB52_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05713.1"
                     /translation="MEADLQLASGALHEAGTIETFLGLDGCSVKVPDPLKTLASAFAV
                     LVCFLALPGFASADEFFINTLEDETNGPFACAEISGDCSLRDAIERGNESTTEDDVVN
                     FEAVLGGPIALGSQLPPITDTLLI"
BASE COUNT          857 a         1928 c         2049 g          926 t
ORIGIN      
        1 gtcaggagcc gaaggatgcg acggggaatc agagagccat tgcgcataga aactggacgg
       61 atgtatgagt aatacccgga attgggaaaa aacgcagaaa aaattggtta acacgcacgg
      121 gcgctggccc tgggtggccg cggcgctcgt cgctcccacc tgggcctcgg cgctggcccg
      181 gcgtcgcgag ctggccgagg gcgacgcctg gatcgccgcc ctggcgctgc cgatcctggt
      241 cgcccaccag accgaggagt gggtgcggcc cggcggcttc ctgcccttct gcaacgaacg
      301 cctgctcggc agtcgcgccc ccgactggcc gctgaccgag cgcctcggct tccacgtcaa
      361 cgtcaccctc ggctggggca gcgccgtcgc cgccaccgcg ctctggcgcc gcagcgccgc
      421 gcccgccgcc ttcgtgctct ggatcgaggc cggcaacgcg ctgatgcacg gcggcatggc
      481 gctgcgcgag cgccgctaca accccggcct cgccaccgcg ctcgggctga tggcgccgca
      541 tgcggcgctc ggcacggtgc ggctgcgccg ctctggccgg ctctcgcgcc gcgcggcggt
      601 gctcgccgcc gcggccggga tcgggttcag cgctgccctg ccggtggcga tgaaggcgcg
      661 cctgcggcgg ggctgagccg cgcgcaccag accgcgaaga agccgaccgc gacgaccgcg
      721 ccggcccagt gcaggccgat cacgacgaag agcaggctgg gcagcccggc gagcacggcg
      781 ccgagcaggg tcggcgccgg cggcagccgc agcacccgcg cggtgagcac ggcgcagagc
      841 agccaggcga gcgggccgga gatccagccg tagtcctcga agaagctctt cggcagggcc
      901 agcgccagcg cgatcgagag caccgcgacc gcgaccagct gcaccagcgc ggcgcgccag
      961 agaatcccgg tgtcgatgcc gctcaccgcc gggccgccct actcgggctt gccccagcgg
     1021 ttgtcgccgt agttgcggtc gcggctgtcc tcgaccgcgc cgtccccgcc gtagccggcc
     1081 tggaccagga gccggcgcag gttgacgatc atctgctcgt cgacgtcctt ctcgcgcggg
     1141 cgatgcaggg tctcggtctc cgggccgacg gtcaccttca cgtggccgtc gtgctcctcg
     1201 ctgacctcgc cgaccgcctc cagcagcgag agaaccgctc gccactcgac gttgccgctc
     1261 gccgggtggg cgaggatctt ctcgaccgtc gcgcggtggt ggtggctcag ctcaggcatc
     1321 ggcgccgaac cctattgggt cggccgccgc gactactgga cgacgatctt gccccgcatt
     1381 tccgggtgga tcgtgcagtg gtagcggaag gtcccggtgc ggttgaagcg gaccgagaac
     1441 tggttgcccg gccggatccg gccgctgttg aagccgccgc cgttgcgggt tgcggtgtgg
     1501 gcggtgttgc tctcgttctc gaactcgacc ttggtcccct tgccgaccgt cagggtcccg
     1561 ggcttgaagg cgaagttgac gatgccgacc ttcttgctgc cgtcgccctg ggcgacgccg
     1621 gcggcctgga tcgagaggag ggagacgagg acgagggtga gcagcgcgat cttcttcatg
     1681 gccccttata cggagcgggc cacggccagg ttcagcagaa gaagtcgtct tcttcgcaga
     1741 gttcttcttc gaagccgccg atgttcgaga cttcgtcttc gagctgctgg acccgttctt
     1801 cgagttcggc cgagccttcg acctggccgc gcagttcttc gacttccgcc tgcacccctt
     1861 caaccgcggc ttcccccgag ggcccgcgcg gacccttcgg tccctgtggc ccggccttgc
     1921 cctgcttgcc cgcaggacct tccggcccgg tctgggcgat caaggccacg gcggtgccga
     1981 tcccggcgct gaggatgcag acgccgacga tcagcgcgat gaagtgattg cgctgcaggg
     2041 agatcatgcg cggcggaatc atcgcagcta ccctgaacga gcccgggcgg ttagctcagt
     2101 tgggagagca cttcccttac aaggaagggg tcactggttc gagcccagta ccgcccacta
     2161 cgcctggcgc ggcagccgtg ccaggcggga gacggtgagc agcgggttgc cgtcgccggg
     2221 ctggaagttg acgttgaggc ggagggtgag cggggccttg gcgaggcggc ggcgggtcgc
     2281 ctcggagagg cggcagtgca ggatggcggg accggcctgg acttgcttcg ccgcgaccgt
     2341 gcaggcgcgg cggcgatcgt cggccgggcc ggtgctgccg atcagcgaga gcgcgccagg
     2401 gccgctgacg gtggcgcgca cgatcaccag gccgcgaccg aggcgggagc ggtcgagctc
     2461 caggcggctg tcgatcgtga acggctgtgg gaccttcgga atctcttcga acggattcgg
     2521 tttgccgggg tcctctttcg gctcttcttc ttcttcaggt tcttcttcgt tcggctcttc
     2581 gggcagctcg ccggtgccga gccagtcggc cagcacctgc ggccgtttgg cgaggaattt
     2641 cagcgtcttc tcgaattcct cttcgatgaa ctcgaggtcg aagggggcgc gctctgactc
     2701 ctcgacttct tgctcctgcc agggggcgag cagggcggcg agcgctttcg cctggtccgg
     2761 caggtcgagc gccacggacg tttcctcgac ggtttccagc gctttgacgt aggccgcgta
     2821 acaggcttcc tcggccaggc actggttgaa cagcagcccg cctttgctgg tgaacgagta
     2881 ccaccagtcg ctcagcgtct ggtcggtccc ggagggcagc atctggaaga caccggccgg
     2941 atcgctgtag aggtagtagt tgttcgggag gccgccgctg tagccgtccc actggccgag
     3001 gtagcgctcg gccgcccaca tcgcgaccat ctcctcgaga tcggcgaccg gcgccagccg
     3061 cggggtgaag ctcggttcgg tcgcgttggc cgcggtgatc agcgcttcca ggtcggtgcg
     3121 ggttttgctt ttgccctcgt cgacctcgta ctcggccgcc atcccgggtt cgaggtcctg
     3181 cccggcgccg ccctcgtaga gatgctgcgg cgggttctgg aacgcgccga agcgcttttc
     3241 cagcgcgatt ttgtcccaga cctcgaggtt caggtgcagc ccatagtcga tcccgttcaa
     3301 gtagacgtag gcgtagccgg tgtggggggc ggggacgccg gcggcgcgga aggcgggata
     3361 gacgagcgcc tcgtgaacca tcgaggggtc ctgggccatg ttgttgaggg tcagcttctc
     3421 cagcccgagg aaggtctgat cgtcgacgaa ctcgtcgaac ttgactttga aggcggcttt
     3481 tttgtcgagc cctttgaacg atccggcgcc ccccttcagc cgcaccccga ccgtcagcgg
     3541 ctcggtgtac gggccgatgc cctccggcgt gccgtcggtt tcggcgatcc ggaactcgcc
     3601 ttcgacgtac tcgtcgggtt cggcttccag cgccgcgacc gccgcttcgc tgagtttgag
     3661 gtcgatgacg acgaccgtgt ccggttcgta cagcgtctcc gcgctcagcg cggcgctcgc
     3721 cgcgggggcg aagacgagga gggcgagcgt tgcgagcgcg gccgcgatca gtcgcggatc
     3781 agccatcgtt cctcgctaac accctgggct cgctgttggc tgcggcggtg cccggtgacc
     3841 gcgagcagga actgctcacg gtcgagtgcc agcagccggg tgtcgacggt cgtccgcacg
     3901 gtcgccgtgc ggggaacgtc gtgcagaagg gcgatctcgc cgaacgattc gcccgggccc
     3961 tcgttgcggc ggaactcgcc gtgttcgaag acctcgactt gtcctgcctc gatcaggtag
     4021 aagcggtcgc cgatgtcgcc ctggacgatc acgtcctgac cagctggcgc atcgaccgcg
     4081 accagatcgt ggctgagccg ctcgacggtg gcgatcggca gcggcgcgaa gatcgagttg
     4141 ccgcgcagca gctggtactg ctcctcggcg accggggcgc cgatctcgta ggagcgcagc
     4201 cgggtccagc agatgaagac gaagaccggc agcagcagcc cgaccacgat cagggcgccg
     4261 tcggggtgga gcagttcgac cagggcgatc gcgaggatcg agccgagcgc ctgcgccccc
     4321 agccgccccg attcgagcgc gccggtcagc cggcccatcg tctcgtcgtc gccgagccgg
     4381 tgcatcagcg tcttcgccgc gacctcgacg tacgtgaacc cgatgccgat cccgaaccag
     4441 ccgatgtagg aggtcacctg ctccggccag atccccggga tcagagtggc gacgccgatc
     4501 acgaggctgc cgccggcgat cgcgacgacc agccggccgc ggtcgagcag cagcgccagc
     4561 gaagcgccgc cgaccagcgc gccgattccc caggccgcgt tgaggaagcc gaccgagccc
     4621 tcctcgaggt gcagcaggtg cagcgccagg gtgacgagga tgacgtcggc gaggccctcg
     4681 aacgccagca gcacgaccag ggtcagcgag gcgagccgca gccccgggtt atcgcgcagg
     4741 gcgcgggcgc cgcgcgaggc ttcgcgcagg atcccggcga cgtcctcctc gtcctcgtat
     4801 tcggggcggc tgtcgcgctt gaccgcgagc agcagcaggg cgacgacgac ggtgaccgcg
     4861 gcggcgaccg cgaacacggt cccgggcgag gtcgccgtga gcaggaagcc ggtggcgatc
     4921 gcggcgacga tgaagccggc gttttccatc gccacgtggt tgacattgct ggccgagagc
     4981 tcctgcgggg tgcgggcgta gaccgggaac agcgccgcct cggccggcat gtaggtggcg
     5041 agcgcgatcg cgtagagcgc cgggaagatg aagacgacgt cggtcgaggc gtcctggctg
     5101 gcggcgaagg cggccccggc gagcaccacc gccatcacgg cgctggcgcg cacgagcagg
     5161 ttgcggcgcg agtagcggtc ggcgagcagc ccgcagaagg gcgagacgat cgcgcccggc
     5221 agcagccgga tcagggcgac gagaccgacg ccgaccaccc cgtgcgcctc gaacccgtag
     5281 acgccgaggg cgatcgcgaa gctccagttg gcgaaggaga tcccgatccg cgcgactccg
     5341 agcagaccga ggtcgcggtt ctcgaggtcg atcatcagag cgtggaggct gatcttcagc
     5401 ttgcgagcgg cgctctgcat gaggcgggga cgatagagac ttttttgggt ctggacggtt
     5461 gttcggttaa ggtgcccgat cctttgaaga cgctcgcgtc agccttcgcc gtgttggtct
     5521 gcttcctggc tctgcctgga ttcgcgtcgg cggacgaatt cttcatcaac accctcgagg
     5581 acgaaacgaa cggcccgttc gcctgtgccg aaatctccgg cgactgctcg ctgcgcgacg
     5641 cgatcgaacg cggcaacgag agcacgaccg aagacgacgt cgtcaacttc gaagcggtcc
     5701 tcggtggtcc gatcgcactc ggcagccagc tgccgccgat caccgacacg ctcctgatcg
//
LOCUS       DASRIG010000181         8044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3209031, whole genome shotgun sequence.
ACCESSION   DASRIG010000181 DASRIG010000000
VERSION     DASRIG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3209031"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFB52_04955"
     CDS             <1..578
                     /locus_tag="VFB52_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05714.1"
                     /translation="AHPPRIYAFGDSVMIGARDQLAARLGPGFSMNAEVGRQADEFVA
                     LVQQLQREGKTPNAVIIQMGNNGPLYGEDMEAIQKATANVGELFLINDHAPVSWIDES
                     NSAIEEAGEDWPHTTVIDWAAAADANEDLLWDGIHLKPAAAALYARLVNEAVREKVAF
                     PPPPKPDPKAKAKKQGQASKQSAAKSSQAPL"
     gene            complement(797..1489)
                     /locus_tag="VFB52_04960"
     CDS             complement(797..1489)
                     /locus_tag="VFB52_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05715.1"
                     /translation="MRKRIAATSFAVGLIGLVAVTSAFAAMVGVYRNEMGSTAARAQM
                     IKISGASCARGGSTDTLKVTIGKKTDACSFRTPVIGRDLEIGATERLLSGTPKAVQRK
                     AYLGLELRAGGGAKYQLLVYPTQRKAQLIKYTAAGPEYFDIAKDLRSVMGVNKANKLR
                     LRALQIRTGPEKGRTSVVGFIGSERVVEGIDAVPGDLAGEASTVAVGAAKNGNGVVAS
                     IDDVVVRTPVHF"
     gene            1584..3578
                     /locus_tag="VFB52_04965"
     CDS             1584..3578
                     /locus_tag="VFB52_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HZO05716.1"
                     /translation="MPDHRLPERDRPWVMRTYAGHSDARRSNELYRGNLAKGQTGLSI
                     AFDLPTQTGYDPDHVLARGEVGKVGVSVAHKGDMHQLLDQIPLGEMNTSMTINATAAW
                     LLGLYVTVAEETGVERSELTGTTQNDIIKEYLSRGTYAFPPEPSMRLIADMVAFSVNE
                     VPQWNPTNICSYHLQEAGATPVQEIAYAMSTAIAVLDEVKARGQVADEDFPKVFGRIS
                     FFVNAGIRLVEEIAKLRTMGALWHELGRERYGVTDEKMLRFRYGVQVNSLGLTEAQPE
                     NNIQRIVLEALAVTMSRDARARALQLPAWNEAMGLPRPWDQQWSLRIQQVLANETDLL
                     EYPDIFEGSVVMDGLVAELTAGAKEEMAVVEERGGAVQAVPYMKARLVESHRERVGRI
                     ERGDQKVIGQNSYTETEESPLTAGADGGILTPDPEVERERIEALEQWKAERDQAAVDA
                     ALEELGRVAADESQNIMPATIAAAKAGATTGEWAAVLREAFGAYRAPTGVDGAAPPAD
                     REQLAEIRQQVDEVSEQIGHRIKILVGKPGLDGHSNGAEQVALRARDSGMEVIYQGIR
                     LTPEEIAESALQEDVDVVGLSILSGSHLELIPEVVRLLQSEGVDVPVVVGGIIPAADA
                     ERLREAGVAGVYTPKDFDVNRIMGEIVALVGRRAAAAAAA"
     gene            3655..4221
                     /locus_tag="VFB52_04970"
     CDS             3655..4221
                     /locus_tag="VFB52_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05717.1"
                     /translation="MSELNGDRSAWLRCYRCWSQNLEIQIHYDAIRRVDPNTGVLADG
                     VDEVQESVVQCLACMHDQPHLTFEDDRVVPIEDRWERMVAGTPWVASCTVTVDQDRVE
                     TCSGPEAIEFLTYGAFGDAGVREFFTHVRFHKHDDEQIVVHLLVELYARSAQEATEVL
                     EEAAHGPLEITSLAEESRPPAHASDEAH"
     gene            4523..6004
                     /locus_tag="VFB52_04975"
     CDS             4523..6004
                     /locus_tag="VFB52_04975"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO05718.1"
                     /translation="MESSTVNQPEREARHAEQAERADFGPQVEKGGPRRRGMVLGRML
                     FLGDVIAAICAGGVALVVFGAASLADAVIFLGAIGLLWPFAAFSIGLYRSDQLASWAS
                     AVSEVPRGFVGVLLITWPTFGIASLAGLNPPGGVAFVTVGSIAALGALSRSVVRASLH
                     RDPNLRQRTLILGSGLVAGQVVEKIRNNPQFGLIPVGIVDDDVHDVGTPDLPWLGRFT
                     DLGAIIEAQNIDRVIIAFSRVSHEDLLEAIRASRDAGVAVDVVPRLFEFLDGVRSLDQ
                     IGGLPLLSIGVPQLASSSVAAKRVLDVVGSSALILLFSPLMIATAIAIKIESRGPVFF
                     RQPRAGRGNKSFKLIKFRSMYTDAEQKKKELKELNEAEDGVMFKIREDPRVTRVGRTI
                     RRFSIDELPQLFNVLKGEMSLVGPRPLIFPETAALEKHWHLRRLELRPGITGPWQVYG
                     RSQSPFQEMVRFDYQYVAGWSLARDIEILLATLPAVMSGRGAY"
     gene            5991..7133
                     /locus_tag="VFB52_04980"
     CDS             5991..7133
                     /locus_tag="VFB52_04980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO05719.1"
                     /translation="MAPTNGAEPVLVHDYLLVMRGAERTFAEMAKCWPQAPISTLLYD
                     PPAVADGFAGREVRTSYLQRLGAKQSRFRYLLPLFPRAAEHLPVSGHQVVVTSSSAFA
                     QGVRPDPGALHVCYCHSPFRYAWFEQERAVAETPAPARPLMKRTLKRFRAWDLEASER
                     VTHYVANSGITRERIAEIYGREADVLHPPVAVERFSVGTPQDYLLFVGQLVRHKRVEV
                     AIEAARQAGRPIKIVGAGPDLPRLQELHADAPVDFLGSVSDAELAELYAGCAALVVPN
                     VEEFGIAAVEAQAAGRPVVAIDRGGVRETVVDGETGILVPGEDAGALAEALRETDFER
                     FDPAAIRAHAERFSAANFRRSLLELVARYSGEEVPSRESATSGGRP"
     gene            complement(7076..>8044)
                     /locus_tag="VFB52_04985"
     CDS             complement(7076..>8044)
                     /locus_tag="VFB52_04985"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZO05720.1"
                     /translation="EKFAGRVRVVERAVPDDTPVSPKLTRNVIFGGLIGLVLGLALAL
                     LLEQLDRRVKRQEELPQVTGLPLLATIPKRQAFDREHLGDGSLSPAEAEIFLMLRANL
                     RYFNVRKDIRSVLVTSATPGEGKSLISLGLALGAAMGGERALLIEADMRDPSLTGAVH
                     KSSAKGLSTVLADPKGDLSDAVVSVEARGIAALAGDAKLDFLPAGPIPPNPTELVASA
                     RMKGLLARAESEYDFVIIDTPPILVVADAIPLVSMVSGVLAVSGLGVSTQTSANDLAE
                     QLERMDAPTLGLVANFATDTGRSYEGYGYGRPPEVALSREGTSSPE"
BASE COUNT         1263 a         2859 c         2727 g         1195 t
ORIGIN      
        1 ccgcccaccc gccgcggatc tacgccttcg gcgactcggt gatgatcggc gcccgcgacc
       61 agctcgccgc caggctcggc cccggcttct cgatgaacgc cgaagtcggc cgccaggccg
      121 acgagttcgt cgccctggtc cagcagctgc aacgcgaagg caagacgccg aacgcggtga
      181 tcatccagat gggcaacaac gggccgctct acggcgaaga catggaagcg atccagaagg
      241 cgaccgcgaa tgttggagag ctgttcctga tcaacgacca cgccccggtc agctggatcg
      301 acgagtccaa ctcggcgatc gaggaagccg gcgaggactg gccccacacg accgtgatcg
      361 actgggccgc ggccgccgac gcgaacgaag acctgctctg ggacggcatc cacctgaagc
      421 cggcggcggc cgcgctctac gcgcgcctcg tcaacgaagc cgtgcgcgaa aaggtcgcct
      481 tcccaccccc gccgaagccc gatccgaagg cgaaagcaaa gaaacagggg caggcgagca
      541 aacaaagcgc cgcgaagtcc agtcaggccc cgctctagga gcacggcgcc atccgcaccc
      601 cagccccgcc tcgtgagcca gccgggatcc ttcggggcgg cctgggtgga ggggtcgggg
      661 tgtccctgga acgccttgta cttttttgct tccgcgaatc gaggcgtaaa gccgcatccg
      721 ccccacaaaa aagttaagtg gaagggacac cccgacccct ccccgctaac caccccgaag
      781 aaccccgcct gactgactag aagtggaccg gggtgcggac gacgacgtca tcgatgctgg
      841 cgaccacgcc gttgccgttt ttggcggcgc cgacggcgac ggtcgaggct tcgccggcga
      901 ggtcgccggg gacggcgtcg atgccttcga cgacgcgctc ggagccgatg aatccgacca
      961 cggaggtgcg gcctttttcg gggccggtgc ggatctgcag ggcgcgcagg cgcagtttgt
     1021 tggctttgtt gacgcccatc acggagcgca ggtccttggc gatgtcgaaa tattcggggc
     1081 ctgcggccgt gtacttgatc agctgggcct tgcgctgggt cgggtagacg agcagctggt
     1141 acttggcgcc gccgccggcc cgcagctcga ggccgaggta ggccttgcgc tggaccgctt
     1201 tcggggtgcc gctgagcagg cgctcggtcg cgccgatttc gaggtcacgg ccgatcaccg
     1261 gggtgcggaa cgagcaggcg tcggtttttt tgccgatcgt cactttcagg gtgtcggtcg
     1321 agccgccgcg cgcgcagctg gcgccagaga tcttgatcat ctgcgcccgc gccgcggtcg
     1381 agcccatctc gttgcggtag acgccgacca tcgccgcgaa ggcgctggtg acggccacga
     1441 gcccgatcag gcccacggcg aagctggtgg cggcgattcg cttacgcatg gggcgtcagc
     1501 agcctaaaca caggctcccc ggaaagatgc gggtccgcag cccctgaacg gcggatttcg
     1561 gccggccaaa tatcattcgg aacatgcctg accaccgact gccggagcgc gaccgtccct
     1621 gggtgatgcg cacttacgcc ggccactccg atgcccgccg ttccaacgag ctctaccggg
     1681 gcaacctggc gaagggccag accggtctct cgatcgcctt cgatctgccc acccagaccg
     1741 gctacgaccc ggaccacgtg ctcgcccgcg gcgaggtcgg caaggtcggc gtctcggtcg
     1801 cccacaaggg cgacatgcac cagcttctcg accagatccc gctcggcgag atgaacacgt
     1861 cgatgacgat caacgcgacc gccgcctggc tgctcggcct ctacgtcacc gtcgccgaag
     1921 agactggggt cgagcgctcc gagctgaccg ggacgacgca gaacgacatc atcaaggagt
     1981 acctctcgcg cggcacctac gccttcccgc ccgagccctc gatgcggctg atcgccgaca
     2041 tggtcgcctt cagcgtcaac gaggtcccgc agtggaaccc gaccaacatc tgcagctacc
     2101 acctgcagga ggccggcgcg accccggtgc aggagatcgc ctacgcgatg tcgaccgcga
     2161 tcgccgtgct cgacgaggtg aaagcgcgcg gccaggtcgc cgacgaggac ttccccaagg
     2221 tcttcggccg gatcagcttc ttcgtcaacg ccggcatccg cctggtcgag gagatcgcga
     2281 agctgcgcac gatgggcgcg ctctggcacg agctcggccg cgagcgctac ggcgtcaccg
     2341 acgagaagat gctgcgcttc cgctacggcg tgcaggtcaa cagcctcggc ctgaccgagg
     2401 cccagccgga gaacaacatc cagcggatcg tgctcgaggc gctggcggtg acgatgagcc
     2461 gcgacgcgcg cgcgcgggcg ctgcagctcc ccgcctggaa cgaggcgatg ggcctgcccc
     2521 gcccctggga ccagcagtgg agcctgcgga tccagcaggt gctggccaac gagaccgacc
     2581 tgctcgagta ccccgacatc ttcgagggct cggtggtcat ggacggcctg gtcgccgagc
     2641 tgaccgcagg cgccaaggag gagatggccg tggtcgaaga gcgcggcggc gcggtccagg
     2701 cggtccccta catgaaggcc cgcctggtcg agtcccaccg cgagcgggtc ggccggatcg
     2761 agcgcggcga ccagaaagtg atcggccaga acagctacac ggagaccgag gaatcgccgc
     2821 tgaccgcggg tgccgacggc ggcatcctca cccccgaccc cgaggtcgag cgcgaacgga
     2881 tcgaggcgct ggagcagtgg aaggccgagc gcgaccaggc ggcggtcgac gccgcgctcg
     2941 aggagctcgg ccgggtcgct gcggacgaga gccagaacat catgccggcg acgatcgccg
     3001 ccgccaaggc cggcgcgacg accggcgagt gggccgcggt cctgcgcgag gccttcggcg
     3061 cctaccgggc gccgaccggc gtcgacggcg ccgccccacc ggccgaccgc gagcagctcg
     3121 ccgagatccg ccagcaggtc gacgaggtct ccgagcagat cggccaccgg atcaagatcc
     3181 tggtcggcaa gcccggcctc gacggccact ccaacggggc cgagcaggtc gccctgcgcg
     3241 cccgcgactc cggcatggag gtgatctacc aggggatccg cctcaccccc gaggagatcg
     3301 ccgagtcggc gctgcaggag gacgtcgacg tggtcggcct ctcgatcctc tccggctccc
     3361 acctggagct gatccccgag gtcgtgcggc tgctgcagtc cgagggcgtc gacgtgccgg
     3421 tcgtggtcgg ggggatcatc cccgccgccg acgccgagcg cctgcgcgaa gccggggtcg
     3481 ccggcgtcta cacgccgaag gatttcgacg tcaaccggat catgggcgag atcgtcgccc
     3541 tggtcggtcg gcgcgccgcc gccgccgcgg ctgcctgatc cttgtaaagg gcttcacaaa
     3601 gcggatcgcc gggtcgcctg gcgccgagcg cacgcgctcg ccacaatggg aggcgtgagc
     3661 gagttgaacg gggaccggtc tgcctggctg cgctgctatc gctgctggtc gcagaacctc
     3721 gagatccaga tccattacga cgcgatacgc cgcgtcgacc ccaacaccgg ggtgctcgcc
     3781 gacggggtcg acgaggtcca ggaatcggtc gtccagtgcc tcgcctgcat gcacgaccag
     3841 ccgcacctga ccttcgaaga cgaccgcgtg gtgccgatcg aagaccgctg ggagcggatg
     3901 gtcgccggca ccccctgggt cgcctcctgc acggtcaccg tcgatcagga ccgggtcgag
     3961 acctgctcgg gcccggaggc gatcgagttc ctgacctacg gcgccttcgg ggacgcgggc
     4021 gtgcgcgagt tcttcaccca cgtccgtttc cacaagcacg acgacgagca gatcgtcgtc
     4081 cacctcctgg tcgagctcta cgcgcgctcg gcccaggagg cgaccgaggt gctcgaagag
     4141 gcggcccacg ggccgctcga gatcacctca ctcgcggagg agtcgcggcc tcccgcgcat
     4201 gcctccgacg aggcccacta actccgctct ccaatgctga attacgcagt ttccgcagat
     4261 cgcaacgcct tgggggtctc gaccggtcaa atatgtgtcc gcaccgggcg ggcgggtccg
     4321 ggactgcaag gtcgattgcc gatcatcagc gcagcacgat ctctgccgag gtgttgatgg
     4381 ctgtggagaa tttcccaaga cccgactatt ttctggaggt acgcagaaac ggtttcgccc
     4441 ccgaaaccgc tgcggtcccc ccaaccttcc gtttcatccc ctcttttcga tccaatgaaa
     4501 gcacggcagc gtagggacca tcgtggagtc ttcgaccgtc aatcagccag aacgcgaagc
     4561 gcggcacgcc gaacaggctg agcgcgccga tttcgggccc caggtggaga agggcggccc
     4621 ccgccgtcgc gggatggtgc tcggccgcat gctcttcctc ggcgacgtga tcgcggcgat
     4681 ctgcgccggc ggcgtagcgc tcgtcgtctt cggcgccgcc agcctcgccg acgcggtgat
     4741 cttcctcggc gcgatcggcc tgctctggcc cttcgcggca ttcagcatcg ggctctaccg
     4801 ctccgaccag ctcgcttcct gggcctcggc cgtctccgag gtcccgcgcg gcttcgtcgg
     4861 cgtgctgctg atcacctggc cgaccttcgg catcgcctcg ctggccgggc tcaacccgcc
     4921 cggcggcgtc gctttcgtca ccgtcggctc gatcgccgcg ctcggggcgc tctcgcgctc
     4981 cgtcgtccgc gccagcctgc accgcgaccc caacctgcgc cagcgcaccc tgatcctcgg
     5041 ctccggcctg gtcgccggcc aggtggtcga gaagatccgc aacaaccccc agttcgggtt
     5101 gatcccggtc gggatcgtcg acgacgacgt gcatgacgtc ggcacccccg acctgccctg
     5161 gctgggccgc ttcaccgacc tcggcgcgat catcgaggcg cagaacatcg accgcgtgat
     5221 catcgccttc tcgcgggtca gccacgagga cctgctggag gcgatccgcg cctcgcgcga
     5281 cgccggcgtc gccgtcgacg tcgtcccccg cctcttcgag ttcctcgacg gcgtccgctc
     5341 gctcgaccag atcggcggcc tgccgctgct ctcgatcggc gtcccacagc tcgcttcctc
     5401 ctcggtggcg gccaagcggg tgctcgacgt ggtcggctcc agcgccctga tcctgctctt
     5461 ctcgccgctg atgatcgcca ccgcgatcgc gatcaagatc gagtcgcgcg ggcccgtctt
     5521 cttccgccag ccgcgcgccg gccgcgggaa caagagcttc aagctgatca agttccgctc
     5581 gatgtacacc gacgcggagc agaagaagaa agagctgaag gagctgaacg aggccgaaga
     5641 cggcgtcatg ttcaagatcc gcgaggaccc acgcgtgacc cgcgtcggcc gcacgatccg
     5701 ccgcttctcg atcgacgagc tcccgcagct gttcaacgtg ctgaagggcg agatgagcct
     5761 ggtcggcccg cggccgctga tcttcccgga gaccgcggcg ctggaaaagc actggcacct
     5821 gcgccggctc gagctgcgcc ccggcatcac cggcccctgg caggtctacg gccgctccca
     5881 gagcccgttt caggagatgg tccggttcga ctaccagtac gtcgccggct ggtcgctggc
     5941 ccgtgacatc gagatcctgc tggcgacgct gccggccgtg atgtcgggcc gtggcgccta
     6001 ctaacggcgc cgagccggtc ctcgtccacg actacctcct ggtcatgcgg ggggcggagc
     6061 gcactttcgc ggagatggcg aagtgctggc cgcaggcacc aatctccacc ctgctctacg
     6121 acccaccggc ggtcgccgac ggttttgcgg gacgcgaggt gcgcacttcc tacctgcagc
     6181 gcctgggagc caagcagagc cgcttccgct acctgctgcc gctcttcccc cgcgccgccg
     6241 agcatctgcc cgtcagcggc caccaggtcg tcgtcaccag cagcagcgcc ttcgcccaag
     6301 gggtccgccc cgaccccggc gcgctgcacg tctgttactg ccatagcccc ttccgctatg
     6361 cctggttcga gcaggagcgg gcggtggcgg agacgccggc gccggcgcgg ccgctgatga
     6421 agcgcacgct gaagcgcttc cgcgcctggg acctggaggc atccgagcgg gtcacccact
     6481 acgtcgccaa ctccgggatc acccgcgagc ggatcgccga gatctacggc cgcgaggccg
     6541 acgtcctgca cccgccggtc gcggtcgagc ggttctcggt gggaacgccg caggactacc
     6601 tgctgttcgt cggccagctg gtccgccaca agcgggtcga ggtcgcgatc gaggcggccc
     6661 gacaggccgg gcggccgatc aagatcgtcg gcgccgggcc cgacctaccg cgcctgcagg
     6721 agctgcacgc ggacgccccc gtcgacttcc tcggcagcgt ctccgacgcc gagctggccg
     6781 agctctacgc cggctgtgcg gcccttgtcg tgcccaacgt cgaggagttc gggatcgccg
     6841 cggtcgaggc gcaggcggcg ggccggccgg tggtcgcgat cgaccgcggc ggcgtgcgcg
     6901 agaccgtggt cgacggcgag accgggatct tggtgcccgg cgaggacgcg ggcgcgctcg
     6961 ccgaggcgct gcgcgagacc gacttcgagc gcttcgaccc ggccgcgatc cgcgcccacg
     7021 cggagcgctt cagcgccgcc aacttccgcc gctcgctgct cgagctggtc gcccgttact
     7081 cgggcgagga agtgccctcg cgcgagagcg cgacctcggg cggacggccg tagccgtagc
     7141 cctcgtagga gcggccggtg tcggtcgcga agttggcgac caggccgagg gtcggcgcgt
     7201 ccatccgttc gagctgctcg gcgaggtcgt tggccgaggt ctgggtgctg accccgaggc
     7261 cgctgaccgc gagcacgccg ctgaccatcg agaccagcgg gatcgcgtcg gcgaccacca
     7321 ggatcggcgg ggtgtcgatg atcacgaagt cgtattcgct ttcggcccgc gccaagaggc
     7381 ccttcatccg cgccgaggcg accagctcgg tcgggttcgg cggaatcggg ccggcgggca
     7441 ggaagtcgag cttggcgtcg ccggccagcg ctgcgatccc ccgcgcctcg acgctgacga
     7501 cggcgtcgga gaggtcgcct ttgggatcgg cgagcacggt gctgaggcct ttcgcggagc
     7561 tcttgtggac ggcgcccgtg agcgatgggt cgcgcatgtc ggcctcgatc agcagcgccc
     7621 gttcgccgcc catcgcggcg cccagcgcga gaccgagcga gatcagcgac ttgccctccc
     7681 ccggcgtcgc cgaggtgacc agcaccgaac ggatgtcttt acgcacgttg aagtagcgca
     7741 ggttggcgcg cagcatcaag aagatctccg cctcggccgg cgataacgag ccgtcaccga
     7801 ggtgctcgcg gtcgaacgcc tggcgcttgg ggatagtcgc cagcagcggc aggccggtta
     7861 cctgtggcag ctcctcctgg cgcttcacgc ggcggtcgag ctgctccagc agcagcgcca
     7921 gggcgagacc cagcaccagg ccgatcagcc cgccgaagat cacgttgcgg gtcagtttcg
     7981 gcgagaccgg cgtgtcgtcg ggaaccgcgc gctcgaccac gcgcacccgg ccggcgaatt
     8041 tttc
//
LOCUS       DASRIG010000182         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_85767, whole genome shotgun sequence.
ACCESSION   DASRIG010000182 DASRIG010000000
VERSION     DASRIG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85767"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFB52_04990"
     CDS             <1..1057
                     /locus_tag="VFB52_04990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO05721.1"
                     /translation="ANGKTRMALERAFGYDIDSDPDRSPYLREARDTGKPVTTPVLEL
                     ALGGPGVNVFQAVYRDGAPRKTVAQRREALIGFVTGSFRAADLLATATGNLPGEVETQ
                     LVFDGETVFGEELDEAAATPIEIAGRTLVLVVRDPDESDLSLPLLLAVVGLAIAVLLG
                     ALIFVWSHNEKLQQLQREASQDPLTGLKNRRRFDEDLRTAMARARRERSTGAMLMLDL
                     DHFKQVNDDHGHPAGDRLIVEIANVLRRRTRESDSLGRLGGDEFAVVLPRCSREEAIL
                     TAEAIATAIREHEPEGEGVEPVTACVGVAMFGTDPRQGAASVASQADAAMYAAKDGGR
                     DGVRIFDPESVAEDHPH"
     gene            complement(1062..2183)
                     /locus_tag="VFB52_04995"
     CDS             complement(1062..2183)
                     /locus_tag="VFB52_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZO05722.1"
                     /translation="MDRTKARRRRVAFGVLLGVALGAFALGAALGDSGTPQAEVEVAT
                     TLPLQQLAGQRVVAGLQGTGISPRLRTAVREGRIAGVVLFEASFPSRAAGRRLIAGLQ
                     AIKRPPKLRDPLLIMVDQEGGLVKRFDGAPTLSARQMGNRGAAVSREQGQRTAANLRS
                     LGVNVDLAPVLDVARPGGVIAETERGFGATAAKVEATAIPFAQALQAGGVAATAKHFP
                     GFGAAGENTDFAVETIDRSKARLRAVDEAPFGAFVEAGGDLVMLSTAIYPAFSDQPAA
                     FSRPIATGELRGRLGFEGVSVTDALGTVAVEDFGGPAKAGLAAVRAGVDLLLFNDLNG
                     AESGWKALTAKLRARKLKRAEFVASAQRVLDLRAELRRR"
     gene            2202..3323
                     /gene="bioB"
                     /locus_tag="VFB52_05000"
     CDS             2202..3323
                     /gene="bioB"
                     /locus_tag="VFB52_05000"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZO05723.1"
                     /translation="MPSLITAEEALAYGALTEHEEIEALVERAWEVRRENFGNSTDMC
                     SLVNAKSGGCAEDCGFCAQSRYAEADTPMHAMMEPEQILEHAEAAEAAGAHRFCMVTQ
                     GQGLSKKDFTKILAGAELVAEKTNLKRCASIGHMSAERAKALKDAGIQRVHHNVESAR
                     SYYPEVSTTVRYEGRLRTIQAVKEAGLETCVGGILNLGETPEQRVEMAFELSEINPTS
                     VPINLLIPKDGTKFGDRTVMDPWEVVQWIAIFRLVIPGALFRLCGGRVENLGSMHKLA
                     VKAGLNGVMMGNFLTTLGAEPAEDRAMFEELGLNVARQPDNGKNPRPDNRSGWLEGET
                     PATPMDELIDHQDEAPMWDPSTQLRVVKKGKAPSYPTAA"
     gene            3320..>3713
                     /locus_tag="VFB52_05005"
     CDS             3320..>3713
                     /locus_tag="VFB52_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO05724.1"
                     /translation="MTDVAQRLEELRERGLHRRLSLIEGPQGPRVTLDGREVLLLCSN
                     NYLGLADDERVREAAAEAARRWGAGSGASRLISGNMDVHRQLEARLAEFLGSETALLF
                     GSGYLANTGAIAALAGRDEVIFSDELNHA"
BASE COUNT          588 a         1346 c         1307 g          472 t
ORIGIN      
        1 ggcgaacggc aagacgcgga tggcgctgga acgcgccttc ggctacgaca tcgactccga
       61 ccccgaccgc agcccctacc tccgcgaggc ccgcgatacc ggcaaaccgg tgacgacccc
      121 ggtgctcgag ctggcgcttg gcggtccggg cgtcaatgtc tttcaggcgg tgtaccgcga
      181 cggggcaccg cgcaagaccg tggcccagcg gcgcgaggcg ctgatcgggt tcgtgaccgg
      241 tagcttccgc gccgccgacc tgctggcaac cgcgaccggc aacctccccg gcgaggtcga
      301 aacccagctc gtcttcgacg gcgagacggt attcggcgag gagctcgacg aagccgccgc
      361 gacgccgatc gaaatcgccg gccgcaccct cgtcctggtc gtccgcgacc ccgacgaatc
      421 tgacctcagc ctgccgctgc tgctggccgt ggtcgggctc gcgatcgccg tcctgctcgg
      481 cgccctgatc ttcgtctgga gccacaacga aaagctccag cagctgcagc gcgaggcgag
      541 ccaggatccg ctgaccgggc tgaagaaccg gcgccgcttc gacgaagacc tgcgcaccgc
      601 gatggcgcgg gcgcgccgcg agcgcagcac cggggcgatg ctgatgctcg acctcgacca
      661 cttcaagcag gtcaacgacg accacgggca tccggccggc gaccggctga tcgtcgagat
      721 cgccaacgtg ctgcgccgcc gcacccgcga gagcgactcc ctcggccgcc tcggcggcga
      781 cgagttcgcc gtcgtcctgc cccgctgcag ccgcgaggag gcgatcctga ccgcggaagc
      841 gatcgccacc gcgatccgcg agcacgagcc cgaaggcgag ggcgtcgagc cggtcaccgc
      901 ctgcgtcggc gtcgcgatgt tcggcaccga cccgcgccag ggcgccgcct cggtcgcctc
      961 acaggcggac gcggcgatgt acgcggccaa ggacggcggc cgcgacggcg tccgcatctt
     1021 cgacccggaa tcggtcgcgg aggaccaccc ccactagccg ctcagcgtcg ccgcagctcg
     1081 gcgcgcaggt cgaggacgcg ctgggccgag gcgacgaact cggcccgttt gagcttgcgg
     1141 gcgcggagct tggcggtcag cgccttccag ccggattcgg cgccgttcag gtcgttgaag
     1201 aggagcaaat cgacacctgc ccggaccgcg gcgaggccgg ccttggcggg gccaccgaag
     1261 tcctcgaccg cgacggtgcc gagagcgtcg gtgaccgaga cgccctcgaa gccgagccgt
     1321 ccgcgcagct cgccggtcgc gatcgggcgg gagaaggcgg cgggctggtc ggagaaggcc
     1381 gggtagatcg ccgtgctcag catcaccagg tcgccaccgg cctcgacgaa ggcgccgaag
     1441 ggggcctcgt cgacggcgcg cagccgcgcc ttcgagcggt cgatcgtttc gaccgcgaag
     1501 tcggtgtttt caccggcggc gccgaagccg gggaagtgct tggcggtggc cgcgacgccg
     1561 cccgcctgca gcgcttgcgc aaacgggatc gcggtcgcct cgaccttggc cgcggtggcg
     1621 ccgaagccgc gttcggtctc ggcgatcacg ccgccgggcc gggcgacgtc gagcaccggc
     1681 gcgaggtcga cgttgacccc gagcgagcgc aggttggcgg cggtccgctg gccctgttcg
     1741 cggctgaccg cggcgccgcg attgcccatc tgccgggccg agagggtcgg tgctccgtcg
     1801 aaccgcttca ccagcccgcc ctcctggtcg accatgatca gcagcgggtc gcgcagcttc
     1861 ggcggccgtt tgatcgcctg cagtccggcg atcagccggc ggccggcggc gcggctggga
     1921 aagctcgctt cgaagaggac gacgccggcg atccggcctt cgcggaccgc cgtgcgcagg
     1981 cgcggcgaga tacccgtccc ctgcagcccg gcgacgaccc gctggcccgc cagctgctgc
     2041 agcggcaggg tcgtggcgac ctcgacctcg gcctgcggcg tcccgctgtc gccgagcgcc
     2101 gcgcccagag caaaggcccc aagcgcgacc ccaagcaaaa ctccaaaggc cacccggcgg
     2161 cggcgcgcct tcgtccgatc catagtgcga tgctatggcc aatgccatcc ctgatcaccg
     2221 ccgaggaggc cctcgcctac ggcgcgctca ccgagcacga ggagatcgaa gccctggtcg
     2281 aacgggcctg ggaggtccgc cgcgagaact tcggcaactc gaccgacatg tgctcgctgg
     2341 tcaacgccaa gtccggcggc tgcgccgagg actgcggctt ctgcgcgcag tcgcgctacg
     2401 cggaggccga cacgccgatg cacgcgatga tggagcccga gcagatcctc gaacacgccg
     2461 aagccgccga ggccgccggc gcccaccgct tctgcatggt cacccagggc caggggctct
     2521 cgaaaaaaga cttcacgaaa atcctcgccg gcgccgaact ggtcgccgag aagaccaacc
     2581 tgaagcgctg cgcctcgatc ggccacatgt cggccgagcg cgcgaaagcg ctcaaagacg
     2641 ccgggatcca gcgcgtccac cacaacgtcg agtcggcgcg ctcctactac cccgaggtct
     2701 cgaccacggt ccgctacgag ggccggctgc gcacgatcca ggcggtcaag gaggccggcc
     2761 tggagacctg cgtcggcggc atcctcaacc tcggcgagac ccccgagcag cgggtcgaga
     2821 tggccttcga gctctccgag atcaacccga cctcggtgcc gatcaacctg ctgatcccca
     2881 aggacgggac caaattcggc gaccgcacgg tgatggaccc gtgggaggtc gtgcagtgga
     2941 tcgcgatctt ccgcctggtc atccccggcg cgctcttccg cctctgcggc ggccgggtcg
     3001 agaacctcgg ctcgatgcac aagctggcgg tcaaggcggg cctcaacggg gtgatgatgg
     3061 gcaacttcct gaccacgctc ggggccgagc cggccgagga ccgggcgatg ttcgaggagc
     3121 tcgggctgaa cgtcgcgcgc cagcccgaca acggcaagaa cccgcgcccc gacaaccgct
     3181 ccggctggct cgagggcgag acgccggcga ccccgatgga cgagctgatc gaccaccagg
     3241 acgaggcccc gatgtgggac ccctcgaccc agctgcgcgt agtgaagaag ggcaaggcgc
     3301 cctcctaccc gaccgctgcg tgaccgacgt cgcccagcgg ctggaggagc tccgcgagcg
     3361 ggggctccac cggcgtttga gcctgatcga ggggccgcag gggccgcggg tgacgctcga
     3421 cgggcgcgag gtgctgctgc tctgctcgaa caactacctc ggcctcgccg acgacgagcg
     3481 cgtgcgcgag gcggcggcgg aggcagcccg gcgctggggt gccggctcag gagcctcgcg
     3541 actgatctcg ggcaacatgg acgtgcaccg gcagttggag gcgcggctgg ccgagttcct
     3601 cggcagcgag acggcgctgc tgttcggctc cggctacctc gccaacaccg gcgcgatcgc
     3661 ggcactggcc ggccgtgacg aggtgatctt ctccgacgag ctgaaccacg cca
//
LOCUS       DASRIG010000183         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1393498, whole genome shotgun sequence.
ACCESSION   DASRIG010000183 DASRIG010000000
VERSION     DASRIG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393498"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFB52_05010"
     CDS             <1..531
                     /locus_tag="VFB52_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05725.1"
                     /translation="VHVADEATEQVKTFSAAGAFVSGFSAEGAAGLTLDCRGAFFVSD
                     YDGGADSSKVHRYGEPGTAAAPCLLPVVVTVLPSNRFKFGKLKLNRKAGTATLTVSVP
                     APGKLVLKGNGLVKATKSAAKAGIVKLAVKPKGKLKRKLGELGKAVAKAKLTFTPSGG
                     TALTKAKSLTLKQKLG"
     gene            544..1767
                     /locus_tag="VFB52_05015"
     CDS             544..1767
                     /locus_tag="VFB52_05015"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05726.1"
                     /translation="MTRRFLILLASALALLGAFAASASAAPGAYRVLLAEVTPIGAKQ
                     LGAQLSAFPDVAAVDYVDTGDEEGVTPTAAQLAPYDVVVSIGDSDYLDVEGWGNSLAA
                     YVGQGGVVVQSAYDTWEDPQAFPQGAFSAGGYAPFIPGKNVNKYTILGTFDPANPLMQ
                     GVTSLVTNGYNTENLLSPGASLVASWANGLPAVAVKGRVVATSAYIGDGYGTEAWSGD
                     FGRLVLNSVRTLGRQLLSVGVFNPAGGKVTSSVGGLDCGTVCTATLAPGTAVSLKATP
                     AKKFAFAGFGGACTGKSCALTMAGGTKAVTATFVAFKPNKKVQLNKQQGKGTLTLYLG
                     GPGKLVLSGKKVKKQTKSPKKVGKVKLPIVAKGKALTQLQNTGKVKVKVSLAYTPAGG
                     AKATTTKSVVLKLAG"
     gene            complement(1777..1974)
                     /locus_tag="VFB52_05020"
     CDS             complement(1777..1974)
                     /locus_tag="VFB52_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZO05727.1"
                     /translation="MSLRLPEDQAAELAAVARADDMPVSEVVREAIDNHIAARRADKD
                     FQKRLKRRLEEDREVLERLAR"
     gene            2107..2502
                     /locus_tag="VFB52_05025"
     CDS             2107..2502
                     /locus_tag="VFB52_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZO05728.1"
                     /translation="MSNKLNLADLLVIAEAVLDERAEDLLRSILVGSAESALAAPFAS
                     FDGVDFYDDPAERAAICCSRIIRNHPFPDGNKRVAYECMREMLERDGLPWPRPSEDAQ
                     EIGDMVEGLAARDVSESEFVAWVKKRTAR"
     gene            complement(2499..2828)
                     /locus_tag="VFB52_05030"
     CDS             complement(2499..2828)
                     /locus_tag="VFB52_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HZO05729.1"
                     /translation="MKVTARARSRDDRAGEPLDGLVNLFDLGIVLSVAFLLAALSSLN
                     LTEAITGKEAQNKNQAANTVTADKNDKVKQVKIKPGEKVVGRGEAIGTVYELSDGRTV
                     IVREPQR"
     gene            complement(2825..3487)
                     /locus_tag="VFB52_05035"
     CDS             complement(2825..3487)
                     /locus_tag="VFB52_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZO05730.1"
                     /translation="MIEDLLFDVSEALKWPVLIAVLLALLVAIIEVGALLVEIARRRH
                     RNVWALERAVEACRAFLGAGQRTEALGALLAVSHSRQMEEAIAGIFDQLGRPDAEARI
                     SKRLAEYDYLSLRRLERTRILVRFGPALGLMGTLIPLSPALAGLADGDVQTLTDNLRV
                     AFGVTVAGLLVGAIAFTVSLLRDRLYAQDYSDVEYIAASLLSGQNPQLPVGQHSHWAV
                     AP"
     gene            complement(3484..>3696)
                     /locus_tag="VFB52_05040"
     CDS             complement(3484..>3696)
                     /locus_tag="VFB52_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05731.1"
                     /translation="GTTTTPPTQEQQLAEARSGNLDDSNGGGGGGVPAAAWGGLATLG
                     LLGLGGLLEARGLGGLMPGRRHGGIA"
BASE COUNT          604 a         1226 c         1280 g          586 t
ORIGIN      
        1 gtccacgtcg ccgacgaagc gactgagcag gtgaagacct tcagcgccgc cggcgcgttc
       61 gtctccggat tcagtgccga aggcgccgcc gggctgacgc tcgactgccg cggcgccttc
      121 ttcgtcagtg actacgacgg cggcgccgac tcctcgaaag tccaccgcta cggcgagccg
      181 ggaacggcag cggcgccctg cctgctgccg gtggtcgtca ccgtgctgcc ctcgaaccgg
      241 ttcaagttcg gcaagttgaa gctgaatcgc aaggcgggca cggcgacgct gaccgtcagc
      301 gtccctgccc cggggaagct ggtgctgaag ggcaacggcc tggtcaaggc aacgaagagc
      361 gcggccaagg ccggcatcgt gaagctcgcg gtgaagccga agggcaagct gaagcggaag
      421 ctcggtgagc tgggcaaggc ggtcgcgaag gcgaagctga ctttcacccc gagcggcggc
      481 acggcactga ccaaggcgaa gtcgctgacc ttgaagcaga agctcggcta ggattctgca
      541 ccgatgacgc gccgttttct catcctgctt gcgagcgcgc tcgcgctgct cggcgccttt
      601 gccgcatcgg cgtcggccgc ccccggcgca taccgggtgc tgctcgccga agtcaccccc
      661 atcggggcga aacagctggg cgcgcagctc agcgcgttcc ccgacgtggc ggcggtcgac
      721 tacgtcgaca ccggcgacga ggaaggagtc acccccaccg cggcgcagct cgcgccctac
      781 gacgtcgtcg tcagcatcgg cgacagcgac tacctcgacg tcgaaggctg gggcaactcg
      841 cttgccgcct acgtcggtca gggcggcgtc gtcgtgcagt ccgcctacga cacctgggag
      901 gacccgcagg ccttcccgca gggggcgttc tcggccggcg gctacgcacc cttcatcccc
      961 ggcaaaaacg tcaacaaata caccatcctc ggcacgttcg acccggcgaa cccgctgatg
     1021 cagggcgtca ccagcttggt caccaacggg tacaacacgg aaaacctgct cagtcccggt
     1081 gccagcctgg tcgccagctg ggcgaacggg ctccccgcgg tcgcggtcaa gggccgcgtc
     1141 gtcgccacca gcgcctacat cggtgacggc tacggaacgg aagcctggtc gggcgacttc
     1201 ggccggctcg tgctcaactc cgtgcgcacg ctcggccgcc agctgctcag cgtcggcgtc
     1261 ttcaacccgg ccggcggcaa agtgaccagc agcgtcggcg gcctcgactg cggcacggtc
     1321 tgcaccgcca cccttgctcc cggcacggcg gtcagcctca aagccacccc cgcgaagaag
     1381 ttcgccttcg ccggctttgg cggcgcctgc accggcaaat cctgcgcgtt gacgatggcg
     1441 ggcggcacca aagcggtcac ggccaccttt gtcgccttca agccgaacaa gaaagtccag
     1501 ctcaacaagc agcagggcaa gggcacgctg accctctacc tcggcggccc cggcaagctc
     1561 gtgctcagcg gcaaaaaggt gaagaagcag accaagtcgc cgaaaaaggt cggcaaggtc
     1621 aaactgccga tcgtcgccaa gggcaaagcc ctgacccagc tgcagaacac cggcaaggtc
     1681 aaggtgaagg tgagtctcgc ctacaccccg gccggcggcg ccaaggcgac gacgaccaag
     1741 tcggtcgtcc tcaagctcgc cggctagcgc ctcgcgctat cgcgcgaggc gttcgaggac
     1801 ctcgcggtcc tcttcgaggc gccgcttcag gcgcttctgg aagtccttgt cggcgcgccg
     1861 cgccgcgatg tggttgtcga tcgcttcgcg cacgacctcc gagacgggca tgtcgtcggc
     1921 gcgggcgacg gccgcgagct cagccgcttg atcctcgggc agtcggagac tcatggcctt
     1981 tccagacatt ggttcagggc tcctggtttt tcatcacggt tgattggtat ggggattcca
     2041 ccagtagatt tgattctacc atctttggtg tattgatacc gaacttttct atatattggt
     2101 atctaaatgt ctaacaaact gaatctcgcc gaccttctag taattgctga ggcagtgctg
     2161 gacgagcgcg ccgaggatct gctgcgctcg atcctcgttg gcagcgctga gtctgcgcta
     2221 gcggcgccct tcgccagctt cgacggcgtc gatttctacg acgaccctgc cgaacgcgcc
     2281 gcgatctgct gctcgcggat catccgcaac caccctttcc ctgacgggaa caagcgcgtc
     2341 gcctacgagt gcatgcggga gatgctcgaa cgggatgggt tgccttggcc gcgcccgagc
     2401 gaggacgcgc aggagatcgg tgacatggtc gaaggtcttg ccgcccgcga cgtctccgag
     2461 agcgagttcg tcgcttgggt gaagaagcgg acggcgcgct agcgctgcgg ttcgcggacg
     2521 atcacggtgc ggccgtcgct gagttcgtag acggtgccga tcgcctcacc gcggccgacg
     2581 accttttcgc cgggtttgat cttcacctgt ttgaccttgt cgttcttgtc ggcggtgacg
     2641 gtgttggcgg cctggttttt gttctgcgct tccttgccgg tgatcgcctc ggtcaggttg
     2701 agcgaggaga gggcggcgag caggaaggcg acggagagca cgatgccgag gtcgaagagg
     2761 ttgacgaggc cgtcgagcgg ttctccggcg cggtcgtcgc ggctgcgggc gcgggccgtg
     2821 actttcacgg cgccaccgcc caatggctgt gttggccgac cgggagctgg gggttctggc
     2881 cgctgagcag ggaggccgcg atgtactcga cgtcggagta gtcctgggcg tagagccggt
     2941 cgcgcagcag cgagacggtg aaggcgatcg cgccgaccag gaggccggcg acggtgacgc
     3001 cgaaggccac gcggaggttg tcggtgaggg tctgcacgtc gccgtcggcg agcccggcca
     3061 gggccgggga gagcgggatc agcgtgccca tcaggccgag cgccgggccg aagcggacga
     3121 ggatgcgggt gcgctcgagg cggcgcaggg agaggtagtc gtactcggcc aggcgcttgg
     3181 agatccgggc ctcggcgtcg ggccggccga gctggtcgaa gatcccggcg atcgcttcct
     3241 ccatctggcg gctgtgggag acggcgagca gcgccccgag cgcctcggtg cgctggccgg
     3301 cgccgaggaa ggcacggcag gcttcgaccg cacgctccag cgcccagacg ttgcggtggc
     3361 ggcggcgcgc gatctcgacc agcagcgccc cgacctcgat gatcgcgacc agcagggcga
     3421 gcaggacggc gatcaggacc ggccacttga gcgcttcgga gacgtcgaaa agcaggtcct
     3481 cgatcacgcg atccccccgt ggcggcggcc gggcatcagg ccgccgagcc cgcgcgcctc
     3541 gagcaggcca ccgagcccga gcaggccgag cgttgccagg ccgccccagg ccgcggccgg
     3601 gacgccgccc ccgccaccgc cgttggagtc gtcgaggttg ccgctgcggg cttcggccag
     3661 ctgctgctcc tgcgtgggcg gcgtggtcgt ggtccc
//
LOCUS       DASRIG010000184         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1902653, whole genome shotgun sequence.
ACCESSION   DASRIG010000184 DASRIG010000000
VERSION     DASRIG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902653"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFB52_05045"
     CDS             <1..2083
                     /locus_tag="VFB52_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZO05732.1"
                     /translation="TNFSRGRDPNADILGGSGPEAKRSRSHPVEPPTLRVPGVGVLRL
                     DPAGFAAWLVPAVAIVYLALNNGGYGVVERSELGIAVWWLLLVGTLVGALPIAGGTNA
                     GRALLIVLAAFAAWTALSLTWTESQERTSIELARTITYLAVFAVALAVQREGRWRQLL
                     NGVTTGVAVVCGIAVLSRLEPTWFEERPSGAFIVGIQIESRLAYPLNYASGLGAFAGI
                     ALPLCLAAASSARTTIGQGLAAAALPIVALTIWLTASGLSVGLVAVTLAIFFFLAPDR
                     LPKLATLLIGAAGSTLLFLAVEQRDALDRGLTNQAAQQEGDELALLILLVCAGVALAQ
                     VGIGLAARHAKRPSWLRVSRQVLTGAAIAIVLVGAPVAVAAGLPAKLSDRWEEFKGED
                     NSSEDNSGGASNARDSRVNQLLNLSGSGRYQFWEAAVDANEAEPLTGIGPGTFEFWWA
                     RNGSYAGFIRDAHSLYMETLGELGIVGFVLIVGFSLAVLIMGAVRCWRAPPDLRLGLA
                     AAVAGCAAFVGGAMVDWTWEIAVFGLVFMVLAAIAVAGGRPDAARRRGGEPPWQRWAG
                     RAAVAVVSLVAIFAIARPLAGTRAIEDSQQDAASGDLQAALAKADDATEIEPYAATPY
                     LQKALVLERLGRPEPAVNAARRATRKESTNWKTWLVLSRLEAEAGNAQAAVDAYLEGR
                     RMNPRSGIFRQ"
     gene            2101..>2702
                     /locus_tag="VFB52_05050"
     CDS             2101..>2702
                     /locus_tag="VFB52_05050"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZO05733.1"
                     /translation="MPRLLAAAVVLVCAGVVLVLEILAVRLAAPYVGLTLETYSAAIG
                     IALLGIAAGAWVGGHLADVVDPRTTLGPTMIVAGVLVLLARPIVDGLGPRLEDGGSLA
                     TLAVVGAGSGPAILALSMAHPAVVKLRLAALEHTGATVGGLSAVGTLGALGGTLLTGF
                     VLLGELSTRDIALGAGLLVVGLGVVVSVALRRSGPHSIVL"
BASE COUNT          325 a          931 c         1013 g          433 t
ORIGIN      
        1 caccaatttc tctcgaggcc gcgacccgaa cgctgacatc ttagggggga gcgggccaga
       61 ggccaagcgg tcgaggtcgc accccgtcga gccgccgacc ctgcgcgttc ccggcgtcgg
      121 cgttctgcgg ctcgacccgg cggggttcgc cgcctggctc gtcccggcgg tcgcgatcgt
      181 ctacctcgcc ctcaacaacg gcggctacgg cgtggtggag cgcagcgagc tcggcatcgc
      241 ggtctggtgg ctgctgctcg tcggcaccct cgtcggcgcg cttccgatcg ccggagggac
      301 gaacgcgggt cgcgctttgc tgatcgtcct cgccgcgttc gccgcgtgga ccgcgctctc
      361 gctgacttgg accgagagcc aggagcgcac ctcgatcgag ctggcgcgca cgatcaccta
      421 cctcgcggtc ttcgccgtgg cgctcgccgt ccaacgcgag gggcgctggc gacagctgct
      481 caacggggtc actaccggtg tcgccgtcgt ctgcgggatc gcggtcctct cgcggctcga
      541 accgacctgg ttcgaggagc ggcccagcgg cgccttcata gtcggaatcc agatcgagag
      601 tcggctcgcc tacccgctca actacgcgag cggactaggc gcgtttgccg ggatcgcctt
      661 gccgctctgc ctggctgctg cctcgtcggc gcgcacgacg atcggccagg gtctggcggc
      721 agccgcgctc ccgatcgtgg cgctgacgat ctggctgacg gcgtccgggc tatcggtcgg
      781 actggtcgcc gtcacgctcg cgatcttctt cttcctcgcg cccgaccgcc tgccgaagct
      841 cgccacgctg ctgatcggcg ccgcgggctc gacgcttctc tttttggcgg tcgagcagcg
      901 cgacgcgctc gaccgggggc tgaccaacca ggcggcccag caagagggcg atgagctcgc
      961 gttgctcatc ctcctggtct gcgccggggt ggcgctcgcc caggtcggaa tcgggctcgc
     1021 ggcccgacac gcgaaacgcc ccagctggct gcgggtctcg cggcaggtgc tgacgggtgc
     1081 cgcgatcgcg atcgtcctgg tcggcgcccc cgtcgccgtc gcggcgggat tgccggcaaa
     1141 gctctccgac cgctgggagg agttcaaggg cgaggacaac agcagcgagg acaacagcgg
     1201 cggcgcgagc aatgcaaggg actcccgcgt caaccagctc ctgaacctga gcgggtcggg
     1261 gcgctaccag ttctgggagg cggccgtcga cgccaacgag gccgagccgc tgaccgggat
     1321 cggccctggg acgttcgagt tctggtgggc tcgcaacggc agctacgcgg gcttcatccg
     1381 cgacgcgcac agcctctaca tggagacgct gggcgaactg ggaattgtgg gcttcgtcct
     1441 catcgtcggc ttctcgctcg ccgtgctgat catgggcgcg gtgcgctgct ggcgcgcgcc
     1501 gccggatctg cgcctcggcc tcgcggcggc ggttgccggc tgcgcagcgt tcgtgggcgg
     1561 ggcgatggtc gactggacct gggagatcgc cgtcttcggg ctcgtgttca tggtgttggc
     1621 ggcgatcgcc gtcgcgggcg ggcggccgga cgcggcacgg cggcgcggcg gtgagccacc
     1681 ctggcagcgc tgggcgggtc gcgccgcggt cgccgtcgtc tcgctggtcg cgatcttcgc
     1741 gatcgctcgt ccgctcgccg gcacgcgcgc gatcgaggac agccagcagg atgctgcgtc
     1801 aggcgatctg caagcggcgc tcgcaaaggc cgacgacgcg accgagatcg agccctacgc
     1861 ggcgaccccc tacctgcaga aggcgctggt gctcgagcgc ctcggccggc cggagccggc
     1921 ggtgaatgcc gcgaggcggg cgaccaggaa ggagtcgacc aactggaaga cctggctcgt
     1981 gctctcgcgg ctcgaggcgg aggccggaaa cgcccaggcg gcggtcgatg cgtatctcga
     2041 ggggcggcga atgaatccgc gatccgggat cttccgccag tgagctcgca gactgcggca
     2101 atgccacgcc tgctcgccgc cgccgtggtg ctcgtctgcg cgggggtcgt cctggtgctg
     2161 gagatcctgg ctgtccggct cgccgccccc tacgtcgggc tcacgctcga gacctactcg
     2221 gcggccatcg ggatcgcgct cctggggatc gccgccggcg cgtgggtagg cgggcacttg
     2281 gccgacgtgg ttgacccccg gacgacgctc gggccgacga tgatcgtcgc cggcgtgctc
     2341 gtgctgctgg cgcggccgat cgtcgacgga ctggggccgc gtctggagga cggtgggtcg
     2401 ctggcgacgc tcgcggtcgt aggggcgggg agcggcccgg cgatcctcgc gctctcgatg
     2461 gcgcatccgg cggtggtgaa gctgcggctc gccgcgctcg agcacaccgg cgcgacggtg
     2521 ggcgggctct cggcggtcgg gacgctgggg gcgctcggcg gcacgctcct gaccggcttc
     2581 gtcctgctcg gagagctctc gacgcgcgac atcgcgcttg gtgccggcct gctggtggtc
     2641 gggctcgggg tggtggtgag cgtggcgctg cgccgcagcg gcccccacag catcgtcctg
     2701 at
//
LOCUS       DASRIG010000185         5291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_858092, whole genome shotgun sequence.
ACCESSION   DASRIG010000185 DASRIG010000000
VERSION     DASRIG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858092"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFB52_05055"
     CDS             <1..509
                     /locus_tag="VFB52_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO05734.1"
                     /translation="ALISAQRRPELVERLVIMDAVPLLPGYRWHWVAQLWRRKPIGEW
                     LNKTTTRSSVALLMRQARGDRSPMPEAFIDMVWEFWDAGQQAAVLSLYRDADPDVLAI
                     AGRDLGRLDCPALVLWGERDAYLPLSFGAAYAHALPQGTFEVARGCGHWPWIDDPGVV
                     DRVADFLA"
     gene            583..1356
                     /locus_tag="VFB52_05060"
     CDS             583..1356
                     /locus_tag="VFB52_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZO05735.1"
                     /translation="MFQRIGSRLPKGWGDLGRQALILVGVDLAYTAVRGIADGERAVA
                     ITHGQQVIDFEQSLHLLFEPDLQAFFLPARWVIDVANQLYLNAQFSVALGFLIWLYLF
                     RNESYYFVRNMFVVSMGLALIGYTLYPTAPPRMYPEYGFVDTINLYSGVNHDSALAKS
                     FINPYAAIPSMHCAFAVMIGGTGAMVCRHWWSRAFWASWPVLILWVVIVTGNHYWIDA
                     VLGWMVAITAFLVARQLLARARPEVWAFRPEGQPREAEI"
     gene            1357..2046
                     /locus_tag="VFB52_05065"
     CDS             1357..2046
                     /locus_tag="VFB52_05065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZO05736.1"
                     /translation="MASSAPPRAPRGGRNGRAQARNGQELRAYARERLIESRLTPNAI
                     SMVGLVGNLVAAVLVTQELFFLAGIAFILGSVMDTLDGRYSRMSGKGTLFGAFLDSTL
                     DRIEEGIVLAAVAGYFAAQGDDIAAAMCVVAVLGSLMVSYTRARAEALGVECKVGLAT
                     RPVRVVLLSIGLIFAKGAGIGDFELLAPAVYVLAALTIFTTFQRVWHVRNELSGAEEA
                     GVASHSDEDDL"
     gene            2107..3285
                     /locus_tag="VFB52_05070"
     CDS             2107..3285
                     /locus_tag="VFB52_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZO05737.1"
                     /translation="MSETNGANGSTNGVGRTDDKVRVAIVGVGNCANSFIQGVQYYKD
                     ADPSEEVPGLMHVDLGGYHVRDVEFVAAFDIDSEKVGKDLGEAIWSGQNDTIKFADVP
                     NLGITVQRGMTHDGLGKYLKEKITKAPGETADVVGILKETKADVLVCYLPVGSEEATK
                     WYVEQALKAGVGFVNCLPVFIAREDYWDKRFEEAGLPIIGDDIKSQVGATIVHRQLAR
                     LFHDRGVRLERTSQLNVGGNMDFYNMLERERLDSKKISKTNAVTSIMGHELDADNVHV
                     GPSDYVPWLTDRKWAHIRLEGKAFGDVPLTAELKLEVWDSPNSAGVVIDAVRLIKLAL
                     NNGISGQLDGPSSYLMKSPHSQRPDDEAREQTEEFIAANARGKSAVPKPADAPSTAQA
                     "
     gene            3375..4631
                     /locus_tag="VFB52_05075"
     CDS             3375..4631
                     /locus_tag="VFB52_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZO05738.1"
                     /translation="MAPRRRAATSSLNDYVDRVSAELVARGHEVLRIETGDPVRRLLG
                     KKSARTIDVVHVHEPFAPSISSAALRHSYALNVATFHAPQERVLSTQVARPLVEIFFG
                     RIDGRTVTSEATAQLLDKYFPANYELIPPAGEGRDWGAVAAEFEALYRGLVARRHPEG
                     GNPELRERISRRPLIEVDLHMHTDHSGDCATPVDVLLQTARDRGLGAIAITDHNEVSG
                     ALEARRIAADFDDLKVIVAEEVKTKEQGEVIGLFLEEKIPKGLTMAETIAAIREQGGL
                     VYVPHPFDRFHSVPDYEHLLDMVEEIDILEVFNPRVALTSFNEEAVRFASKYRIVSGA
                     GSDSHVAQGLGSVRTRIHDFEGPAEFLEAMRDADITRKHKNLVYVQTLKFLQTTGRPK
                     APKRRVPDAKPARGGKPRKRRSTSKS"
     gene            4688..>5291
                     /locus_tag="VFB52_05080"
     CDS             4688..>5291
                     /locus_tag="VFB52_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZO05739.1"
                     /translation="MRGTDDEIREKYLERAIRELNQMSREVQECQHCPRGNLMPVLGS
                     GHPQADVFMLKHSPMSAEIEEGVAFYGRAGNALMKSFKRLGIDPLVVYGTLCVKCPIA
                     EPDLAPQECVARLAEELAIVQPKIVVVMGEPSLRILNGLGIPLSRQIEQREGEIQAFT
                     PTVDALYVPDIDDSLDEEGAKQRFWRAFRMLGTWYEDLPPY"
BASE COUNT          866 a         1870 c         1735 g          820 t
ORIGIN      
        1 tggcgctgat cagcgcgcag cggcgccccg agctggtcga gcggctggtg atcatggacg
       61 cggtgccgct cctgcccggc taccgctggc actgggtcgc gcagctctgg cggcgcaagc
      121 cgatcggcga atggctgaac aagaccacga cccgctcctc ggtcgcgctg ctgatgcggc
      181 aggcgcgcgg cgaccgctcg ccgatgccgg aagcgttcat cgacatggtc tgggagttct
      241 gggacgccgg ccagcaggcg gcggtgctct ccctctaccg cgacgccgac cccgacgtgc
      301 tcgcgatcgc cggccgcgac ctcggccgcc tcgactgccc cgcccttgtc ctctggggcg
      361 agcgcgatgc ctatctgccg ctgagcttcg gcgccgccta cgcccacgcg ctgccgcagg
      421 gaaccttcga ggtcgcccgc ggctgcggcc actggccctg gatcgacgac cccggtgtcg
      481 tcgaccgcgt ggccgacttc ctcgcttagc gcggaatcac gccttttgcg ccacttcgcc
      541 acgagtaacg cgcggattac aatggtcggt tccgtgtcac gcgtgttcca acgcattgga
      601 tcccggctgc ccaagggctg gggcgacctc ggccgccagg cgctgatcct ggtcggagtc
      661 gaccttgcct acaccgcggt gcggggaata gccgacggcg agcgcgccgt cgcgatcacc
      721 catggccagc aggtgatcga cttcgagcag tcgctgcacc tgctcttcga gcccgacctg
      781 caggccttct tcctgcccgc ccggtgggtg atcgacgtcg ccaaccagct ctacctgaac
      841 gcgcagttct cggtcgcgct cggcttcctg atctggctct acctcttccg caacgagtcc
      901 tactacttcg tgcgcaacat gttcgtcgtc tcgatgggcc tcgccctgat cggctacacg
      961 ctctacccga ccgcgccgcc gcggatgtat cccgagtacg gcttcgtcga cacgatcaac
     1021 ctctattcgg gcgtcaacca cgactcggcg ctggccaagt cgttcatcaa cccctacgcg
     1081 gcgatcccga gcatgcactg cgcttttgcg gtgatgatcg gcggtaccgg ggcgatggtt
     1141 tgccgccact ggtggtcgcg cgccttttgg gcgtcctggc cagtgctgat cctctgggtc
     1201 gtgatcgtca ccggcaacca ctactggatc gacgccgtgc tcggctggat ggtcgcgatc
     1261 accgccttcc tcgtcgcccg tcagctgctc gccagagcgc ggcccgaggt ctgggccttc
     1321 cgccccgagg gccagccgcg agaggccgaa atctaagtgg cgtcctccgc ccctccacgg
     1381 gccccgcgcg gcggccgcaa cggccgcgcc caggcccgca acggccagga gctgcgcgcc
     1441 tacgcgcgcg agcggctgat cgagtcgcgg ctgaccccga acgcgatctc gatggtcggc
     1501 ctggtcggca acctggtcgc cgccgtcctg gtcacccagg agctcttctt cctcgccggc
     1561 atcgccttca tcctcggctc ggtcatggac acgctcgacg gccgctactc gcggatgtcg
     1621 ggcaagggga cgctgttcgg cgcgtttctc gactcgaccc tcgaccggat cgaggagggg
     1681 atcgtgctcg ccgccgtcgc cggctacttc gccgcgcagg gcgacgacat cgccgcggcg
     1741 atgtgcgtgg tcgccgtgct cggctcgctg atggtctcct acacgcgcgc ccgcgccgag
     1801 gcgctcgggg tcgagtgcaa ggtcggcttg gcgacgcggc cggtcagggt ggtgctactt
     1861 tcgattgggt tgatcttcgc caagggcgct ggaatcggcg atttcgagct cttggcgccg
     1921 gcggtctacg tgctcgccgc actgacgatc ttcaccacgt tccagcgtgt atggcatgtc
     1981 cgaaacgagc tttccggcgc tgaagaagcc ggtgtcgcca gccacagcga cgaggacgac
     2041 ctgtaaccag cgaggccgct gggtgtgttc acccactcag cgcgatccag gaggaaacca
     2101 gacttcatga gtgagaccaa cggagcaaac ggcagcacga acggcgtcgg ccgcacggac
     2161 gacaaggtcc gcgttgcgat cgtcggcgtc ggcaactgcg ccaactcctt catccagggc
     2221 gtccagtact acaaggacgc cgaccccagc gaggaagtcc ccggtctgat gcacgtcgac
     2281 ctcggcggct accacgtgcg cgacgtcgag ttcgtcgccg ccttcgacat cgactccgag
     2341 aaggtcggca aggacctcgg cgaggcgatc tggtcggggc agaacgacac gatcaagttc
     2401 gccgacgtgc ccaacctcgg catcaccgtg cagcgcggga tgacccacga cgggctcggc
     2461 aagtacctga aagagaagat caccaaagcc ccgggcgaaa ccgcggacgt ggtcggcatt
     2521 ctcaaggaga ccaaggccga cgtcttggtc tgttacctgc cggtcggctc cgaggaggcg
     2581 accaagtggt acgtcgagca ggcgctgaag gcgggcgtcg ggttcgtcaa ctgcctgccg
     2641 gtcttcatcg cccgcgagga ctactgggac aagcgcttcg aagaggccgg gctgccgatc
     2701 atcggcgacg acatcaagag ccaggtcggg gcgacgatcg tccaccgtca gctcgcccgc
     2761 ctcttccatg accgcggcgt gcgcctggag cggaccagcc agctcaacgt cggcggcaac
     2821 atggacttct acaacatgct cgagcgcgag cggctcgact cgaagaagat ctcgaagacc
     2881 aacgcggtca cctcgatcat gggtcacgag ctcgacgcgg acaacgtcca tgtcggcccc
     2941 tccgactacg tgccgtggct gaccgaccgc aagtgggcgc acatccggct cgagggcaaa
     3001 gccttcggcg acgtgccgct gaccgccgag ctgaagctcg aggtctggga ctcgccgaac
     3061 tcggccggcg tcgtgatcga cgcggtacgg ctgatcaagc tggcgctcaa caacgggatc
     3121 tccggccagc tcgacggtcc ctccagctac ctgatgaagt ccccgcacag ccagcggccc
     3181 gatgacgagg cgcgcgagca gaccgaggag ttcatcgccg ccaacgcgcg gggcaagtcg
     3241 gctgtgccga agccggccga cgccccgagc accgctcagg cctaagcgtc cagtcctctc
     3301 gcataggagc ccgctccggc gggctcctat gcttgggtcc taacgtggcc gacgccccgc
     3361 tctccatcgc tcaggtggcg ccccgccggc gcgccgccac ctccagtctc aacgactatg
     3421 tcgatcgcgt ttccgcggag ctggtggcgc gcggccacga ggtgctgcgg atcgagaccg
     3481 gcgatccggt gcggcgtctg ctggggaaaa aaagcgcccg cacgatcgac gtcgtccacg
     3541 tgcacgagcc gttcgcgccg agcatctcct cggcggcgct gcgccactcc tacgcgctca
     3601 acgtcgccac cttccacgcg ccgcaggagc gggtcctctc gacccaggtg gcgcggccgc
     3661 tggtcgagat cttcttcggc cggatcgacg gccgcacggt gaccagcgag gcgaccgcgc
     3721 agctgctcga caaatacttc ccggccaact acgagctgat cccgccggcc ggggaggggc
     3781 gcgactgggg cgcggtcgcc gcggagttcg aagcgctcta ccgcgggctc gtcgcccgcc
     3841 gtcatcccga gggcggcaac ccggagctgc gcgagcggat ctcgaggcgg ccgctgatcg
     3901 aggtcgacct gcacatgcac accgaccact cgggcgactg cgcgaccccg gtcgacgtgc
     3961 tcctgcagac ggcccgcgac cgcgggctcg gggcgatcgc gatcaccgac cacaacgagg
     4021 tctccggggc gctggaagcg cgccggatcg ccgcggactt cgacgacctc aaggtgatcg
     4081 tcgccgagga ggtgaagacg aaggagcagg gtgaggtgat cggcctcttc ctcgaggaga
     4141 agatcccgaa ggggctgacg atggcggaaa cgatcgccgc gatccgcgag cagggcggcc
     4201 tcgtttacgt cccgcacccg ttcgaccgct tccactcggt ccccgactac gagcacctgc
     4261 tcgacatggt cgaggagatc gacatcctcg aggtcttcaa cccgcgggtg gcgctgacct
     4321 cgttcaacga ggaggcggtg cgcttcgcca gcaagtaccg gatcgtctcc ggggcgggct
     4381 ccgacagcca cgtcgcccag gggctcggca gcgtgcgcac ccggatccac gacttcgagg
     4441 ggccggcgga gttcctcgag gcgatgcgcg acgccgacat cacccgcaag cacaagaacc
     4501 tggtctacgt gcagaccctc aagttcctgc agacgacggg ccgcccgaag gcgccgaaac
     4561 ggcgcgtccc cgacgcgaaa ccggcgcgcg gcggcaaacc ccgcaaacgc cgctcgacga
     4621 gcaagagctg atgcagcggt cgggcggcaa aggagcggcg cccgagccgt caaagatgtc
     4681 tccccagatg cgaggcaccg acgatgagat ccgtgagaag tacctagagc gcgcgatccg
     4741 cgagctcaac cagatgagcc gggaggtgca ggagtgccag cactgcccgc gcgggaacct
     4801 gatgccggtg ctcggctccg gccacccgca ggccgacgtg ttcatgctca agcactcgcc
     4861 gatgagcgcg gagatcgagg agggcgtcgc cttctatggc cgggcgggca acgcgctgat
     4921 gaaaagcttc aaacgcctcg gcatcgatcc gctcgtcgtc tacggcacgc tctgcgtcaa
     4981 atgcccgatc gccgagcccg acctggcgcc gcaggagtgc gtcgcccgcc tggccgagga
     5041 gctggcgatc gtgcagccga agatcgtcgt cgtcatgggc gagccctcgc tgcgcatcct
     5101 caacggcctc ggcatcccgc tctcccgcca gatcgagcag cgcgagggcg agatccaagc
     5161 cttcacccca accgtcgacg cgctctacgt ccccgacatc gacgactcgc tcgacgagga
     5221 gggcgccaag caacgcttct ggcgcgcctt ccgcatgctc ggcacctggt acgaggacct
     5281 gccgccgtac t
//
LOCUS       DASRIG010000186         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_87838, whole genome shotgun sequence.
ACCESSION   DASRIG010000186 DASRIG010000000
VERSION     DASRIG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87838"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..478
                     /locus_tag="VFB52_05085"
     CDS             68..478
                     /locus_tag="VFB52_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05740.1"
                     /translation="MIRRIAVVPALVAAVALAACGSSSEEPEASKVTPAAAKASVERA
                     AHVKLASEPVPADAKEEGMAASFSNTSTVVKDKQAVALFVMKDADVANEVSKRVRSSA
                     PKSARLIAHGPVLVVYAAAGKDRGAAVERAVEAL"
     gene            478..1167
                     /locus_tag="VFB52_05090"
     CDS             478..1167
                     /locus_tag="VFB52_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05741.1"
                     /translation="MRSFIRRLLRGHATAVAYVALFAALGGSAYAAATITGANIKDGT
                     VGGRDVKDRSIGANELTQQARDSLASQPGPQGAPGAPGAKGDKGEPGAPGPAGPKGDA
                     GPKGDNGQAGPAGPQGPQGQQGPQGPRGFTGDPGPSGVSGWSYHTAGVTINPDTYEDV
                     SVDCPAGKKALGGGAAASGPYQGNYMNGAIVRSAPSGASATGWTVRYTNGYSMGKMTV
                     YVWAICANVTF"
     gene            complement(1201..>2370)
                     /locus_tag="VFB52_05095"
     CDS             complement(1201..>2370)
                     /locus_tag="VFB52_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05742.1"
                     /translation="DAEFAELLSKALYRRKGFVAWRDEGDEIGDPSLDELIEGGDPVT
                     PLPPDREGRQTILTSGTTGTPKGASRGSPGIGAAVAILSAIPLRGREKVLIAPPLFHQ
                     WGFAHFGLAMLTASTMVLRRKFDPKNVLETIERERIDSVPMVPVMLQRILDLDDEDRR
                     GHDTSSLRTVPVSGSALSGDLAKRFMDEFGDVVYNLYGSTEVAWVAIAGPKDLRDAPG
                     TAGRPPRNTELAILGDDDRPLRTGETGRIFVKNSMLFEGYTGGGSKDMVDGMMATGDV
                     GRLDEEGRLFVEGRDDDMIVSGGENVFPQEVEETLAKHPKVAEAAVIGVEDEKWGQAL
                     KAYVVKKGSVSESALKKHVKENLAGYKVPQQIEFIDELPRNPAGKVLKRELEEDD"
BASE COUNT          328 a          890 c          825 g          327 t
ORIGIN      
        1 gggttctcgc agccgccggg gcggacactc gcgccgaccc ctcctcccct ccccgcaagg
       61 actccccatg atccgccgta tcgccgtcgt ccccgctctc gtcgccgccg tcgcactcgc
      121 cgcgtgcggc tcctcctccg aggagcccga ggcgtcgaag gtcacgcccg cggccgccaa
      181 ggccagcgtc gagcgcgccg cccacgtcaa gctcgcctcc gagccggtgc ccgccgacgc
      241 gaaggaggag ggcatggccg cctccttcag caacacgtcc acggtcgtca aggacaagca
      301 ggccgtcgcg ctgttcgtga tgaaggacgc cgacgtcgca aacgaggtct ccaagcgcgt
      361 ccgctcgtcg gcgcccaagt ccgcgcggct gatcgcccac ggcccggtcc tggtcgtcta
      421 tgcggcggcc ggcaaggacc gcggcgccgc cgtcgagcgc gcggtggagg cgctctgatg
      481 cgctccttca tccgccggct gctccgcggc cacgccaccg ccgtcgccta cgtcgctttg
      541 ttcgccgccc tgggtggcag cgcgtatgcg gcggcgacga tcacgggcgc gaacatcaag
      601 gacggcacgg tcggtggccg cgacgtcaag gaccgctcga tcggcgcgaa cgagctgacc
      661 cagcaggccc gcgactcgct ggcgtcccag cccggcccgc agggcgcgcc cggggcgccc
      721 ggggcgaagg gcgacaaggg cgagccggga gcaccggggc ccgccggccc gaagggcgac
      781 gccggcccga agggcgacaa cggccaggcg ggtcccgccg ggccgcaggg tccacaaggg
      841 cagcagggcc cgcaggggcc gcgtggcttc accggagacc cgggcccgag cggggtcagc
      901 ggctggtcgt accacaccgc cggcgtcacg atcaatcccg acacctacga ggacgtgagc
      961 gtcgactgcc cggccggcaa gaaggcgctc ggcggcggcg ccgcggcgag cggcccctac
     1021 cagggcaact acatgaacgg ggccatcgtc cgcagcgcgc cctccggcgc ctcggcgacg
     1081 ggctggaccg tccgctacac caacggctac tcgatgggca agatgaccgt ctacgtctgg
     1141 gcgatctgcg cgaacgtgac gttctgagcg acccacggcc gccccgggaa cggggcggcc
     1201 tcagtcgtcc tcttccagct cccgcttgag caccttgccg gcggggttgc gcggcagctc
     1261 gtcgatgaac tcgatctgct gagggacctt gtagcccgcc aggttctcct tgacgtgctt
     1321 cttgagggcc gactcggaga cggagccctt cttgacgacg tacgccttca gggcctggcc
     1381 ccacttctcg tcctcgacgc cgatcacggc cgcctcggcc accttcgggt gcttggcgag
     1441 cgtctcctcg acctcctggg ggaagacgtt ctcgccgccc gagacgatca tgtcgtcgtc
     1501 gcggccctcg acgaacaggc ggccctcctc gtcgagccgg ccgacatcgc ccgtggccat
     1561 catcccgtcg accatgtcct tcgagccgcc gccggtgtag ccctcgaaga gcatcgagtt
     1621 cttgacgaag atccggccgg tctcacccgt ccgcagcggg cggtcgtcgt cgccgaggat
     1681 cgcgagctcc gtgttgcgcg gcgggcggcc ggcggtgccc ggggcgtcgc gcaggtcctt
     1741 ggggccggcg atcgcgaccc acgccacctc ggtggagccg tagaggttgt agaccacgtc
     1801 gccgaactcg tccatgaagc gcttggcgag gtcgcccgac agcgccgagc ccgacacggg
     1861 gacggtccgc agcgagctcg tgtcgtgccc gcgacggtcc tcgtcgtcga ggtcgaggat
     1921 gcgctggagc atcaccggca ccatcgggac ggagtcgatc cgctcgcgct cgatcgtctc
     1981 gagcacgttc ttcgggtcga acttgcggcg cagcaccatc gtcgaggcgg tcagcatcgc
     2041 cagcccgaag tgcgcgaagc cccactggtg gaacagcggc ggcgcgatca gcaccttctc
     2101 gcgcccgcgc agcgggatcg cggagaggat cgcgaccgcg gcgccgatcc ccggcgagcc
     2161 gcgcgaggcg cccttgggcg tccccgtcgt gcccgaggtc aggatcgtct ggcggccctc
     2221 gcggtctggc gggagcggcg tgaccgggtc cccgccctcg attagctcgt ccagcgacgg
     2281 gtcgccgatc tcgtcgccct cgtcgcgcca cgcgacgaag cccttgcgcc ggtacagcgc
     2341 cttcgagagc agctcggcga actcggcgtc
//
LOCUS       DASRIG010000187         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_88111, whole genome shotgun sequence.
ACCESSION   DASRIG010000187 DASRIG010000000
VERSION     DASRIG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88111"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFB52_05100"
     CDS             <1..537
                     /locus_tag="VFB52_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05743.1"
                     /translation="MTLDAVCRTFVPRTLGNYFSQQLRWRRSNLVDYVGGMSHVWKLN
                     PLIAIHFFSLFGLLIAYPALIMRAIVTERFFPLMLTHVALATLFGVVYGWRVRKLPAS
                     DKVGLLAFVPIALVLPVTYALLTPLALFTLDSGSWETRGHAPEDSADAEDERCEVPTA
                     VAEMAEPSPVSRLELPAA"
     gene            553..1356
                     /locus_tag="VFB52_05105"
     CDS             553..1356
                     /locus_tag="VFB52_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05744.1"
                     /translation="MPPSDKPTPWNRIAPFLQPTIIRLYKVAGLVALGAILLGLVSYV
                     VLNVFYFFDRTWVRPVILSPQHEKVIETTAALSDARMRHSQIEGEKAAAATELAGLHR
                     LVATNEKFEHDAAAIAAGRTAEAVLVRREVEKSSLERLTALERLTALEKRIADLDVRL
                     REQDAVIATLVASPYIEAVEQRKVLAFVPYKNLEEATPGTVLYGCEWGLVGCSRVGRI
                     LKILDGETQDRNPHDGSGERGQLAEIEMTDRKAAEQNVLFAGSRPFWIF"
     gene            complement(1353..>2059)
                     /locus_tag="VFB52_05110"
     CDS             complement(1353..>2059)
                     /locus_tag="VFB52_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05745.1"
                     /translation="AWSGLPQRAAKEAQSRIDAVAVAVEGLDDEALVLQSDLEALTAA
                     PAGDAISLLSFEDNFLVLHGGPAVVTDPAQHGREVEVWGSSKATTLGEAAHLAHRTVV
                     VGGVVQGFWEVDLDAGTGVWASFGDVDAATRARVDEQVAEAARFLLEDVGHARSFSLD
                     TDEDIRRRAAHVKALGAAKKAAPKKRAVAAAPKTKKTKAPAKKPAAAPKTKSKKPAAK
                     KPAAKKPAAKKPRKKG"
BASE COUNT          265 a          790 c          672 g          332 t
ORIGIN      
        1 atgaccctcg acgccgtgtg ccggaccttc gtgccgcgca ccctcggcaa ctacttctcg
       61 cagcagctgc gctggcggcg ctccaacctg gtcgactacg tcggcgggat gtcgcacgtc
      121 tggaagctga acccgctgat cgcgatccac ttcttctcgc tgttcggcct cctgatcgcg
      181 tacccggcgc tgatcatgcg cgccatcgtg accgagaggt tcttcccgct catgctgacc
      241 cacgtcgcgc tggccacgct gttcggcgtc gtctacggct ggcgcgtgcg caagctgccg
      301 gcgagcgaca aggtcggact gctcgccttc gtgccgatcg cgctggtcct gcccgtgacc
      361 tacgcgctct tgacgccgct cgccctgttc accctcgact ccggcagctg ggagacccgc
      421 ggccacgccc ccgaggattc cgccgacgcc gaggacgagc gctgcgaggt gccgaccgcg
      481 gtcgccgaga tggcggaacc gtcgccggtg tcgcggctcg agctccccgc tgcctagttt
      541 cctggaagcc gtatgccccc ctccgacaag ccgacgccct ggaaccggat cgccccgttc
      601 ctccagccca cgatcatccg cctctacaag gtggccggcc tggtggccct gggagcgatc
      661 ctgctcggcc ttgtcagcta cgtcgtgctg aacgtcttct acttcttcga ccggacctgg
      721 gtccgccccg tcatcctgtc gccgcagcac gagaaggtca tcgagaccac ggccgcgctg
      781 tccgacgccc ggatgcggca cagccagatc gagggcgaga aggcggcggc ggcgaccgag
      841 ctcgccggcc tgcaccggct ggtcgccacc aacgagaagt tcgagcacga cgccgccgcc
      901 atcgccgccg gccgcaccgc cgaggcggtg ctggtccgcc gcgaggtcga gaagagcagc
      961 ctcgagcggc tgaccgccct cgagcggctg accgccctcg agaagcggat cgccgacctc
     1021 gacgtccgcc tgcgcgagca ggacgcggtg atcgcgaccc tggtggcgtc gccgtacatc
     1081 gaggcggtcg agcagcgcaa ggtgctggcc ttcgtgccgt acaagaacct cgaggaggcg
     1141 acgcccggga cggtcctcta cggctgcgag tggggcctcg tcggctgcag ccgggtcggg
     1201 cggatcctca agatcctcga cggcgagacc caggatcgca acccgcacga cggctcgggc
     1261 gagcgcggcc agctggccga gatcgagatg acggatcgga aggcggccga gcagaacgtg
     1321 ctgttcgccg gctcgcgtcc gttctggatc ttctagccct tctttcgcgg cttcttcgcg
     1381 gccggcttct tcgcggccgg cttcttcgcg gctggcttct tgctcttcgt cttcggcgcg
     1441 gccgcgggct tcttcgccgg cgccttggtc ttcttcgttt tcggggcggc cgcgaccgcc
     1501 cgttttttcg gcgccgcctt cttcgccgcg cccagggcct tcacgtgcgc cgcccgccgc
     1561 cggatgtcct cgtcggtgtc gaggctgaag ctgcgcgcgt gcccgacgtc ctcgagcagg
     1621 aagcgcgcgg cctccgccac ctgctcgtcg acccgcgccc gggtcgcggc gtcgacgtcg
     1681 ccgaagctcg cccacacccc ggtcccggcg tcgagatcga cctcccagaa gccctgcacg
     1741 acgccgccga ccacgaccgt ccggtgcgcc aggtgcgcgg cctcgcccag cgtcgtcgcc
     1801 ttcgacgacc cccagacctc gacctcgcgg ccgtgctggg ccggatcggt caccaccgcc
     1861 ggcccgccgt gcaggaccag gaagttgtcc tcgaacgaca gcagtgagat cgcgtccccc
     1921 gccggcgccg cggtgagcgc ctcgagatcg gattgcagga ccagcgcctc gtcgtcgagg
     1981 ccctcgacgg cgaccgcgac cgcgtcgatc cgcgactgcg cctccttcgc cgcccgctgc
     2041 ggcagccccg accacgccg
//
LOCUS       DASRIG010000188         9549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2165058, whole genome shotgun sequence.
ACCESSION   DASRIG010000188 DASRIG010000000
VERSION     DASRIG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165058"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..390)
                     /locus_tag="VFB52_05115"
     CDS             complement(10..390)
                     /locus_tag="VFB52_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05746.1"
                     /translation="MDEFNHAPDDLINFNESVYASGWEASTKMGGWMRLGNRVNEGYA
                     ELSVCLYLPDGRVACQFKRPEIEANDRFEAGGLRYAVKEPFESLEMEYEGELLVLDDP
                     EDLRDPAAMFKTAPRVDGADHLGG"
     gene            553..924
                     /locus_tag="VFB52_05120"
     CDS             553..924
                     /locus_tag="VFB52_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05747.1"
                     /translation="MSSPEVIRAVGEVLKGAAAAGAGDDYQRSQVLSAYSMTRHLAAE
                     ESGRAELTAAFGAELETILGDRDGGGWAAETDPAALGERLSRVLAELRAGDEDSRRTA
                     TALRAALRRLCEREVENLAAA"
     gene            929..1963
                     /locus_tag="VFB52_05125"
     CDS             929..1963
                     /locus_tag="VFB52_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZO05748.1"
                     /translation="MSVEAIEPEGRERAAAALELLRLAGVLGDDADATSLIQLEGGWS
                     RQAYVLGARDEGVEREFVVRVKPPGPLLDTDLRQEYETTRLLTRHGVAAPVAHAFEEG
                     ENPFGGPYYVMDRAPGSAPNVWRARDRAELEANWEAGGSLAEDLVDGLVGIHSIPAAE
                     ATAVLPLRDFEAAVSHWETVQGEMALLRDPVVEDAYAWLREREPEPVEPTLVHGDYRI
                     GNCLNEHGRITAILDWELSYVGDPRYDLGYISLEYEAGSFTKPGSKLLGSVAEREWFM
                     REYERRSGRPVDPEVVRTYAVLADLMLVAILTTGIRMYVDGKSEDVRMAWTRFAIPGL
                     RRELTALMSY"
     gene            complement(1967..2347)
                     /locus_tag="VFB52_05130"
     CDS             complement(1967..2347)
                     /locus_tag="VFB52_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZO05749.1"
                     /translation="MSAEFPSVSEAVSQETIDRYADISGDYNPIHVDLEAAAASEFGG
                     TIAHGPIALQTFFRSLTESTGGGALPPGTKVKVTFRAPVRPGDTVRSELREREDGAGA
                     TELSAECVNQDGTTVIAAQASVPS"
     gene            complement(2344..2781)
                     /locus_tag="VFB52_05135"
     CDS             complement(2344..2781)
                     /locus_tag="VFB52_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05750.1"
                     /translation="MSNEEAPALSNVTYDELEVGATLGTVTEPVSAELAGSLVGAIGE
                     PGEAAVAPPAVFPVLFLKALRRTLGGIPNGGVLAKYELDFGAPLKTGGEAEIKVSIAD
                     KYVRRERPYVVVQFDISDGDGVQVLSGRKIIMWPTGPGEEAPK"
     gene            complement(2794..3114)
                     /locus_tag="VFB52_05140"
     CDS             complement(2794..3114)
                     /locus_tag="VFB52_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZO05751.1"
                     /translation="MYTVIYPLYRKPELSQEEFVSYWLETHKPIAMQIPNVRSYRICP
                     VIEAEGVEGEEVAGFVIFEFDSKADFDEAHDSPEFAATAADASGNFVRHFTRYAVDAH
                     KAIG"
     gene            3250..4854
                     /locus_tag="VFB52_05145"
     CDS             3250..4854
                     /locus_tag="VFB52_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent acyl-CoA ligase"
                     /protein_id="HZO05752.1"
                     /translation="MADSRLQYVAEPGSASTVSALLTARAELTPDAPFVSFEDTTLTY
                     AEIVARADRTAAGLAAMGVGEGTKVAVLLPNSTEILETWFACSLMGAVLVPVNTGLVG
                     EGLKYILEHSEAELLVVDSSLLEVLEAAYSVTDGLGKRFVRGSDESGLPNGYEPYAAL
                     TEAGDSGPRASVGPETLASILYTSGTTGLPKGVMNCHNSYVTAALEFSRNYVRMREDD
                     VLYTSLPLFHVNAQSLTTLGSVVSGRPMVLSPRFSASRFFADLAAHKATVFNYIGAML
                     TMLYKQPEGPHDAENNVRLTVGGAAPAELWRDFEKRFGLEVLEIYGLTESATFCCGSP
                     PDAIKPGRIGKEVGWSEVKVVGPDDEELPADEPGEIVVRSKVPDTMFQGYYKNEEATA
                     ESMKGGWFHTGDRGLRDADGYFAFVDRLKDSIRRRGENISSYEVERVVNAHPEVAESA
                     AYGVPSDLGESDVMVTVVPRGDLDPAELVAFCAERMAKFMVPRYVRVQEALPKTATQR
                     VQKFELRKLGTEGAWDSLASAEQIKR"
     gene            4871..5965
                     /locus_tag="VFB52_05150"
     CDS             4871..5965
                     /locus_tag="VFB52_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05753.1"
                     /translation="MSTVKVAGIGWAQPGEDEQLSHAELLYRATRQALGDAGLKRDAI
                     TSAVTTSYDYVEGRNLSNQFTLDSIGGTMKSCDLRLSDDGIHSIAAGSMEALSEPGGV
                     VVVGAVQMAGTELVDATNRAVEEVLFEPLYQRPILAGAAHPEALVFGLAARAYLARHG
                     VDEEKLAELVAARSAGGAEARTAEEILDSPVIAGPLREGHRARRTDAAATLILTTEAP
                     NGARATIRGTGWAAEDGQFANRDLSEQRPTALAAEQAFAAAGVSDPSAELARVEAYNA
                     YAIDEAMTAEALGLAAPGEGFDALLGGAQVNPTGGVQATGWARGAASLVQAVRAIEEM
                     VDQPGALLASQGWSGPGASSSAVAVFEIDG"
     gene            5999..7171
                     /locus_tag="VFB52_05155"
     CDS             5999..7171
                     /locus_tag="VFB52_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05754.1"
                     /translation="MSKKQRVGVASVGMMPFNRVLEDTAEEMAYKVAKDALDKAGLRR
                     DDIDAWVVGSAVDAFSGVNESDKFLVAGSGGNGKPWFRNATSGSTAVSTPDLAYSMVA
                     SGQVDVVMCICYEKMSDNENSQKVFNYVYDPAWVRPVGLNVPLQCGIEARAYLHRYGI
                     EEEEMAMLSVQSLGNALDNPYAQRGKEITVEDVLESPYISWPVKVLDTSPVSDGARAA
                     IFISEDKLKEIDDDPVYVKGIGRGADSYWFMGRRNNDLGRLDYAYLGADQAYEMAGIK
                     NPSTEVHVAEVYDPFTYKAMQHMEAAGLCPEGGAGKHIREGHGARDGKLPITPSGGLL
                     GEGNPIGAGMSRMTWTWLQVKGQAGDCQVERDVDTAISTGWGGMYQYNATMVIGRD"
     gene            7200..7646
                     /locus_tag="VFB52_05160"
     CDS             7200..7646
                     /locus_tag="VFB52_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZO05755.1"
                     /translation="MSNTEPGVTIDGRVDVEYHWSAGIAGSRFFTELRDNGKIMGTKC
                     PECKRVLVPPRIFCEECFVDTDEWVEVSTSGEILTFAESYFGLQGQRLEEPWYIGIIK
                     LDGADGGLFHRIVKGDSEIEIGGRVEAVLADNREGHIMDIVNFKTV"
     gene            7643..8461
                     /locus_tag="VFB52_05165"
     CDS             7643..8461
                     /locus_tag="VFB52_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO05756.1"
                     /translation="MSDLVHLQRVGDVAEFVFDAPPVNQLSPAFVDELEAAIDGVGDA
                     RAVAITSAVPKIFMAGGDIKLMEGSPVAEQGAYIRRLQATFHALEMLELPVVVGIDGA
                     ALGGGTEIALACDIRIAAADATMGQPESTLGIFPGGGGTHRLVRSVGNTVAMDLMMSG
                     RRISGEEAGGYNLVSRVVGSGEATAAAKELAAELANGATEALRAIKRLGLASYDTPPV
                     QGFAAEAAEWTRVRESANAQEGLSAFLERRTPNFKAPLPPDVEAGRRQPTRRDA"
     gene            8458..>9549
                     /locus_tag="VFB52_05170"
     CDS             8458..>9549
                     /locus_tag="VFB52_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05757.1"
                     /translation="MNFEPSDRAKELRERVRVFMDEHIYPNEREIEEALDAEVDAQTP
                     FPKILLEIRAKAKAEGLWNLALPDEVDGPGLSNLDYGWVSEEIGRCTIPAAYAFNVQP
                     PDSGNMEILVEHARGVQRERWLTPLLEGEIRTCFAMTEPDTAGSDPTGLACRAELDGE
                     DWVINGRKWWTTNAHGAQMAIVMAVTDPDAAPHKRATMLLVPTDTPGYNPVRPLYNMG
                     HADGPGHWEIEFEDCRVPADESTLNERGSGFKVAQDRLGPGRIHHCMRLIGVAERALE
                     MMCERANSREMFGSKLAEKQLVQDFIATSRAEIDQARLLTLHAAWRLDEVGNRAARKE
                     LSITKLVVPTMGLNVVDRAIQVFGGAGVSE"
BASE COUNT         1490 a         3397 c         3298 g         1364 t
ORIGIN      
        1 aacccgatgt catcccccga ggtgatccgc gccgtcgacc cggggcgcgg tcttgaacat
       61 cgcggccggg tcgcgcaggt cctcggggtc gtcgaggacg agcagctcgc cctcgtactc
      121 catctccagc gactcgaacg gctccttgac cgcgtagcgc aggccgccgg cctcgaagcg
      181 gtcgttggcc tcgatctcgg ggcgcttgaa ctggcaggcg acgcggccgt cgggcaggta
      241 gaggcagacc gagagctccg cgtagccctc gttgacgcgg ttgccgagcc gcatccagcc
      301 gcccatcttc gtgctggcct cccagccgct ggcgtagacg ctctcgttga agttgatcag
      361 gtcgtcgggg gcgtggttga actcgtccac cgcctccaga cgcaggccgt tgctggaaat
      421 cgcgatccct ccgttcgtct cgccaatcct ccgggccagc gtaagttcgg cccccgcgcg
      481 ctggaactgg cactagcgcc agtgcctcgc tcccggcgcc gctgctggaa tcgcagggcg
      541 gtgttcaacc cgatgtcatc ccccgaggtg atccgcgccg tcggcgaggt gctgaagggc
      601 gctgccgcgg cgggcgccgg tgacgactac cagcgctcgc aggtgctctc cgcgtactcg
      661 atgacccgcc acctcgccgc cgaggagagc ggccgggccg agctgacggc cgccttcggg
      721 gccgagctgg agacgatcct cggcgaccgc gacggcggcg gttgggccgc ggagaccgac
      781 cccgcggcgc tcggggagcg gctcagccgc gtgctcgccg agctgcgcgc cggcgacgag
      841 gactcgcgcc gcaccgccac cgcgctgcgc gccgccctgc gccgactctg cgagcgcgag
      901 gtcgagaacc tcgccgccgc ctagggccat gtcggtcgag gcgatcgagc cggagggccg
      961 cgagcgcgct gccgccgcgc tggagctgct gcgcctcgcc ggggtgctcg gtgacgacgc
     1021 ggacgccacc tcgctgatcc agctcgaggg cggctggtcg cgccaggcct acgtgctcgg
     1081 cgcccgcgac gagggtgtcg agcgcgagtt cgtcgtccgc gtgaagccgc ccggcccgct
     1141 gctcgacacc gacctgcgcc aggagtacga gaccaccagg ctgctgacca ggcacggcgt
     1201 cgccgccccg gtcgcccacg ccttcgagga gggcgagaac ccgttcggcg gcccgtacta
     1261 cgtgatggac cgggcgcccg gctcggcccc gaacgtgtgg cgcgcccgcg accgcgccga
     1321 gctggaggca aactgggagg ccggcggctc gctcgccgag gatctggtcg acggcctcgt
     1381 cggcatccac tcgatcccgg cggcggaggc gaccgcggtg ctgccgctgc gcgacttcga
     1441 ggccgcggtc tcccactggg agacggtcca gggcgagatg gcgctgctgc gcgacccggt
     1501 ggtcgaggac gcctacgcct ggctgcgcga gcgcgagccc gagccggtcg agccgaccct
     1561 cgtgcacggc gactaccgga tcggcaactg cctcaacgag cacggccgga tcaccgcgat
     1621 cctcgactgg gagctctcct acgtcggcga cccccgctac gacctcggct acatctcgct
     1681 cgagtacgag gccggctcct tcaccaagcc cggctcgaag ctgctcggct cggtcgccga
     1741 gcgcgagtgg ttcatgcgcg agtacgaacg ccgctccggc cgcccggtcg accccgaggt
     1801 cgtgcgcacc tacgcggtgc tggcggacct gatgctggtc gcgatcctca ccaccggcat
     1861 ccgcatgtac gtcgacggca aaagcgagga cgtgcgcatg gcctggaccc gcttcgcgat
     1921 ccccggcctg cgccgcgagc tgaccgcgct gatgagctac tgagcgctag ctcgggaccg
     1981 aggcctgggc ggcgatcacg gtggtgccgt cctggttgac gcactcggcg ctcagctcgg
     2041 tggcgccggc gccgtcctcg cgctcgcgca gctcgctgcg cacggtgtcg ccggggcgga
     2101 ccggggcgcg gaaggtgacc ttgaccttgg tcccgggggg cagggcgccg ccgccggtcg
     2161 actcggtcag ggagcggaag aaggtctgca gggcgatcgg gccgtgggcg atcgtgccgc
     2221 cgaactcgga ggcggcggcc gcctccaggt cgacgtggat cgggttgtag tcgccggaga
     2281 tatcggcgta gcggtcgatc gtctcttggc tgacggcttc ggacacggat gggaactcgg
     2341 cgctcacttg ggggcctcct ctccgggccc ggtgggccac atgatgatct tgcggccgct
     2401 gaggacctgg acgccgtcgc cgtcgctgat gtcgaactgg acgaccacgt aggggcgctc
     2461 gcggcgcacg tatttgtcgg cgatcgagac cttgatctca gcctcgccgc cggtcttcag
     2521 gggggcgccg aagtcgagct cgtatttggc cagcacgccg ccgttcggga tcccgccgag
     2581 ggtgcggcgc agcgccttga ggaagagcac cgggaagacg gccggcggtg ccacagcggc
     2641 ctcgccgggc tcgccgatcg cgcccaccag cgacccagcc agctcggcgc tgaccggctc
     2701 ggtgaccgtg cccagcgtcg cgccgacctc gagctcgtcg taggtgacgt tggagagtgc
     2761 gggtgcttcc tcgttgctca tcgcgttcct tgctcagccg atcgccttgt gggcgtcgac
     2821 cgcgtagcgg gtgaagtgac ggacgaaatt gcccgaggcg tcggccgcgg tggcggcgaa
     2881 ctcggggctg tcgtgggcct cgtcgaagtc ggctttcgag tcgaactcga agatcacaaa
     2941 accggcgacc tcctcgccct cgaccccctc cgcttcgatc accggacaga tccggtagct
     3001 gcgcacgttc gggatctgca tcgcgatcgg cttgtgggtc tccagccagt aggagacgaa
     3061 ctcctcctgg ctcagctccg gcttgcggta gagggggtag atgacggtgt acacgcggct
     3121 cctccgtggg gttggctttg ggccaattcc aaggagtcga cccgggccaa gcctgagcca
     3181 ggggcggcgg tgcgccaact gtcaattgcg ccagcttccg gcgcaggggt tcggtgattc
     3241 gatgctcgcg tggccgattc ccgccttcag tacgtcgccg agcccggctc tgcgagcacc
     3301 gtcagcgccc tgctgaccgc ccgcgccgag ctcactcccg acgccccctt cgtcagcttc
     3361 gaagacacga cgctgaccta cgcggagatc gtcgcccgcg ccgaccgcac cgccgccggg
     3421 ctcgccgcga tgggggttgg agagggcacc aaggtcgccg tgctgctgcc gaactcgacc
     3481 gagatcctcg agacctggtt cgcctgctcg ctgatgggcg cggtcctggt cccggtcaac
     3541 accggcctgg tcggcgaagg cctcaagtac atcctcgagc actccgaggc cgagctgctg
     3601 gtcgtcgact cctcgctgct ggaggtgctg gaggcggcct actcggtcac cgacgggctc
     3661 ggcaagcgct tcgtccgcgg cagcgacgag agcggattgc cgaacggcta cgagccctac
     3721 gcggcgctga ccgaagccgg cgactcgggc ccgcgcgcca gcgtcggccc cgagaccctc
     3781 gcctcgatcc tctacacgtc gggcaccacc gggctgccca agggcgtgat gaactgccac
     3841 aactcctacg tcaccgcggc gctcgaattc agccgcaact acgtgcggat gcgcgaggac
     3901 gacgtgctct acacgagcct gccgctcttc cacgtcaacg cgcagtcgct gaccacgctt
     3961 ggctcggtcg tctccggccg gccgatggtg ctctcgccgc gcttctccgc ctcgcgcttc
     4021 ttcgccgacc tcgccgccca caaggcgacc gtcttcaact acatcggcgc gatgctgacg
     4081 atgctctaca agcagcccga gggcccgcac gacgccgaga acaacgtccg cctcacggtc
     4141 ggcggcgccg ccccggccga gctctggcgc gacttcgaga agcgcttcgg cctcgaggtt
     4201 ctggagatct acggcctcac cgagtcggcg accttctgct gcggcagccc gcccgacgcg
     4261 atcaagccgg gccggatcgg caaggaggtc ggctggtcgg aagtgaaagt cgtcggcccc
     4321 gacgacgagg agctgcccgc cgacgagccg ggcgagatcg tcgtccgctc gaaggtcccg
     4381 gacacgatgt tccagggcta ctacaagaac gaggaggcga ccgcggagtc gatgaaaggc
     4441 ggctggttcc acaccggcga ccgtggcctg cgcgacgccg acggctactt cgcgttcgtc
     4501 gatcggctca aggactcgat ccgccgccgc ggcgagaaca tctcgtctta cgaggtcgag
     4561 cgggtcgtca atgcccaccc cgaggttgcc gagagcgccg cttacggtgt tccctccgac
     4621 ctcggtgagt ccgacgtgat ggtgaccgtg gtcccgcgcg gcgatctcga ccccgcggag
     4681 ctggtcgcct tctgcgcgga gcggatggcg aagttcatgg tcccccgcta cgtgcgcgtc
     4741 caggaggcgc tgccgaagac ggcgacgcag cgggtgcaga agttcgagct gcgcaagctt
     4801 gggaccgagg gggcttggga cagccttgcc tcggccgaac agatcaaacg atgacgagga
     4861 ggacttggtg atgagcactg tcaaggtggc cggaatcggc tgggcgcaac cgggcgagga
     4921 cgagcagctc tcgcacgccg agcttctcta ccgcgccacg cgtcaggcgc tcggcgacgc
     4981 cggcctgaag cgcgacgcga tcaccagcgc ggtcaccacc tcctacgact acgtcgaggg
     5041 ccgcaacctc tccaaccagt tcacgctcga ctcgatcggc gggacgatga agtcctgcga
     5101 cctgcgcctc tccgacgacg ggatccactc gatcgccgcc ggctcgatgg aggcgctctc
     5161 cgagcccggc ggcgtggttg tcgtcggcgc cgtgcagatg gccggaaccg agctggtcga
     5221 cgcgaccaac cgggccgtcg aagaggtcct cttcgagccg ctctaccagc gcccgatcct
     5281 ggccggcgcc gcccaccccg aggcgctcgt cttcggcctc gccgcccgcg cctacctcgc
     5341 ccgccatggg gtcgacgagg agaaactggc cgagctggtc gccgcccgct cggccggcgg
     5401 cgccgaggcg cgcacggcgg aggagatcct cgactcgccg gtgatcgccg gcccgctgcg
     5461 cgagggccac cgcgcccgcc gcaccgacgc cgccgccacc ctgatcctca ccaccgaggc
     5521 cccgaacggc gcccgcgcga cgatccgcgg caccggctgg gccgccgagg acggccagtt
     5581 cgccaaccgc gacctctccg agcagcggcc gaccgcgctc gccgccgagc aggccttcgc
     5641 ggccgccggc gtctccgacc cgagcgccga gctggcccgg gtcgaggcct acaacgccta
     5701 cgcgatcgac gaggcgatga ccgccgaggc gctcggcctc gcggctcccg gcgagggctt
     5761 cgacgccctg ctcggcggcg cccaggtcaa cccgaccggc ggcgtgcagg cgaccggctg
     5821 ggcgcgcggc gccgccagcc tggtgcaggc ggttcgagct atcgaagaga tggtggacca
     5881 gccgggggcg ctgctcgcct cgcagggctg gagcgggccg ggcgcctcgt cgtccgcagt
     5941 cgccgtcttc gagatcgacg gctgagccgc gagacctacg agagagaaag gcaagcaagt
     6001 gagcaagaaa cagcgcgtgg gtgtcgcctc ggtcgggatg atgccgttca accgcgtcct
     6061 cgaggacacc gccgaggaga tggcctacaa ggtcgccaag gacgccctcg acaaagccgg
     6121 cctgcgccgc gacgacatcg atgcctgggt cgtcggctcc gcggtcgacg ccttctccgg
     6181 cgtcaacgag agcgacaagt tcctggtcgc cggctccggc ggcaacggca aaccctggtt
     6241 ccgcaacgcg accagcggct cgaccgcggt ctcgaccccg gacctcgcct actcgatggt
     6301 cgcctccggc caggtcgacg tggtgatgtg catttgctac gagaagatgt ccgacaacga
     6361 gaactcccag aaggtcttca actacgtcta cgaccccgcc tgggttcgcc ccgtcggcct
     6421 caacgtcccg ctgcagtgcg ggatcgaggc gcgcgcctac ctccaccgtt acgggatcga
     6481 ggaggaggag atggcgatgc tctcggtcca gagcctcggc aacgcgctcg acaaccccta
     6541 cgcccagcgc ggcaaggaga tcacggtcga ggacgtgctc gaatcgccct acatctcctg
     6601 gccggtcaag gtgctggaca ccagcccggt ctccgacggc gcccgggcgg cgatcttcat
     6661 cagcgaggac aagctgaaag aaatcgacga cgacccggtc tacgtcaagg gcatcggccg
     6721 cggcgccgac tcctactggt tcatgggccg ccgcaacaac gacctcggcc gcctcgacta
     6781 cgcctacctc ggcgccgacc aggcctacga gatggccggg atcaagaacc cctccaccga
     6841 agtccacgtc gccgaggtct acgacccctt cacctacaag gcgatgcagc acatggaggc
     6901 cgccgggctc tgtcccgaag gcggtgccgg gaagcacatc cgggaagggc acggggcgcg
     6961 cgacggcaaa ctgccgatca cgccgtccgg cggcctgctc ggcgagggca acccgatcgg
     7021 cgccgggatg tcacgcatga cttggacctg gctgcaggtc aagggccagg cgggcgactg
     7081 ccaggtagag cgcgacgtcg acaccgcgat cagcaccggc tggggcggca tgtaccagta
     7141 caacgcgacc atggtcatcg gccgcgacta gcggccgggc aagagaagga gctattgaga
     7201 tgagcaacac tgagcccggc gtcacgatcg acggccgcgt cgacgtcgaa taccactggt
     7261 ctgcgggcat cgccggcagc cggttcttca ccgagctacg cgacaacggc aagatcatgg
     7321 gcaccaagtg ccccgagtgc aagcgggtcc tggtgccgcc gcggatcttc tgcgaggagt
     7381 gcttcgtcga caccgacgag tgggtcgagg tctcgacctc gggtgagatc ctgaccttcg
     7441 ccgagtccta cttcggcctc caggggcagc gcctcgagga gccttggtac atcgggatca
     7501 tcaagctcga cggtgccgac ggcggcctct tccaccggat cgtcaagggc gacagcgaga
     7561 tcgagatcgg cggtcgggtc gaggcggtgc tggccgacaa ccgcgagggt cacatcatgg
     7621 acatcgtcaa tttcaagacc gtatgagcga cctggttcac cttcagcgcg tcggcgacgt
     7681 cgccgagttc gtctttgacg cgccgccggt gaaccagctc tcgccggcct tcgtcgacga
     7741 gctcgaagcg gcgatcgacg gggtcggcga cgcccgggcg gtggcgatca ccagcgccgt
     7801 gccgaagatc ttcatggccg gcggtgacat caagctgatg gagggctccc cggtcgccga
     7861 gcagggcgcc tacatccgcc gcctgcaggc gaccttccac gcgctggaaa tgctcgagct
     7921 ccccgtggtc gtcggcatcg acggcgccgc gctgggcggc ggcaccgaga tcgcgctcgc
     7981 ctgcgacatc cggatcgccg cggccgacgc gaccatgggc cagcccgagt cgacgctggg
     8041 gatcttcccc ggcggcggcg gcactcatcg gcttgtgagg tccgtcggga acaccgtagc
     8101 catggacctg atgatgagcg gccgccggat cagcggcgag gaggccggcg gctacaacct
     8161 cgtcagccgc gtggtcggct ccggcgaggc gaccgccgcc gccaaagagc tcgccgccga
     8221 gctcgccaac ggggcgaccg aggcgctgcg cgcgatcaag cggctcgggc tcgcctccta
     8281 cgacaccccg ccggtccagg gcttcgccgc cgaggccgcc gaatggaccc gggtccgtga
     8341 atccgccaac gcccaggagg gcctcagcgc cttcctcgag cggcggacgc ctaacttcaa
     8401 agcaccgctg ccgcccgacg tcgaggccgg gcgccggcaa ccaaccagga gggacgcatg
     8461 aacttcgagc ccagcgaccg cgccaaggag ctgcgcgagc gcgtccgtgt gttcatggac
     8521 gagcacattt acccgaacga gcgcgagatc gaagaggcgc tcgacgccga ggtcgacgcg
     8581 cagaccccgt tcccgaagat cctgctcgag atccgcgcga aggccaaggc cgagggcctc
     8641 tggaacctcg ccctgcccga cgaggtcgac ggccccgggc tctccaacct cgactacggc
     8701 tgggtcagcg aggagatcgg ccgctgcacg atccccgcgg cctatgcctt caacgtccag
     8761 ccgccggact cgggcaacat ggagatcttg gtcgagcacg caaggggggt ccagcgcgag
     8821 cggtggctga cgccgctgct ggagggtgag atccgcacct gcttcgcgat gaccgagccc
     8881 gacaccgccg gctcggaccc gaccgggctc gcctgccggg ccgagctcga cggcgaggac
     8941 tgggtgatca acgggcgcaa gtggtggacg accaacgcgc acggtgccca gatggcgatc
     9001 gtcatggccg taacggaccc cgacgccgcc ccccacaagc gcgcgacgat gctgctggtc
     9061 ccgaccgaca cccccggcta caacccggtc cgcccgctct acaacatggg ccacgccgac
     9121 gggcccggcc actgggaaat cgagttcgag gactgccggg tccccgccga tgagagcacg
     9181 ctgaacgaac gcggcagcgg cttcaaggtc gcccaggacc ggctcggtcc gggccgcatc
     9241 caccactgca tgcggctgat cggagttgcc gagcgggcgc tggagatgat gtgcgagcgc
     9301 gccaactcgc gcgaaatgtt cggcagcaag ctggccgaga agcagctggt ccaggacttc
     9361 atcgcgacct cgcgggcgga gatcgaccag gcccggctgc tcaccctgca cgccgcctgg
     9421 cggctcgacg aggtcggcaa ccgcgccgcc cgcaaggagc tctcgatcac caagctcgtg
     9481 gttccgacga tgggactgaa cgtggtcgac cgggcgatcc aggtgttcgg cggcgccggc
     9541 gtctccgaa
//
LOCUS       DASRIG010000189         9146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3481669, whole genome shotgun sequence.
ACCESSION   DASRIG010000189 DASRIG010000000
VERSION     DASRIG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481669"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFB52_05175"
     CDS             <1..313
                     /locus_tag="VFB52_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZO05758.1"
                     /translation="PLWGSEDHLRGLFGERVGFESLERDVLEVSAFAQPGEFGDHFRQ
                     RYGPTIVTRANAEREGKLEQLDQGIDRFCSDWNLGSDENARFELEYLLAVGTRAGGEE
                     R"
     gene            316..1728
                     /locus_tag="VFB52_05180"
     CDS             316..1728
                     /locus_tag="VFB52_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO05759.1"
                     /translation="MGAAAEPSAEELGFRLRGGLYEPGDAEYEDACTLFNSMIERRPR
                     LVARCSAPEDVVAALAFGRERGLPIAVRGGGHSVTGRSLCDDGLVLDLRGMRDVEVDP
                     LRRVARVGGGATWADVDGACGEHGLATTGGRVSTTGVAGLTMGGGSGWLERKHGLACD
                     NLLAAQLVTAGGEIVRAAEDENPELLWALRGGGANFGVVTALELALHPIESQVLGGLV
                     LHPAERGPELLRLFRDLIANAPEELGLAFAFITAPDEADVPAELRGQPAVIVAGMYAG
                     PVAEGEAALRELRAFGPPAADYFEPTTYAEFNRSLDDPPGYRNYWTAEHLAELTDEAI
                     EAIARRSAEIPVSPSQLFIVPWGGALTRVDPTSSPLAGRQAAFVVHPLLLWEDEADDA
                     RMIALGRAYRDDLAPFSTGAAYLNFLGDEGEARVRAGFGEENYRRLAAVKERWDPDGV
                     FHGNQSLADGGTCPSPGASV"
     gene            complement(1706..2674)
                     /locus_tag="VFB52_05185"
     CDS             complement(1706..2674)
                     /locus_tag="VFB52_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05760.1"
                     /translation="MATGGGADTDVARGEELFSAGDLDGAQAAFERADAAGDPRGTLK
                     LAALLEDDRHDLAAAEHVWRRADEAGDLNGSGNLGRLLREKGDLRGAEDAFFRCVERG
                     SARALGDWGALLLNREDASAEEIAAMTDRLCGAHDLFIWNKDTSVMGAVMMLDAAAER
                     ADPAAFEAGTRRADERGSASGAWQLAWVLRGRGDLAGAVVAFRRSAERGHGEAWSFGA
                     GTCFELGDPATAEAMAREGDRAGVAKASSMLGSILDERGQPEEAIAAYRRGDEGGDGH
                     GSFNLGISLAQGGDLGGAEAALSRAVERDVDNAAEALAQIRQMRPG"
     gene            complement(2732..2804)
                     /locus_tag="VFB52_05190"
     tRNA            complement(2732..2804)
                     /locus_tag="VFB52_05190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2769..2771),aa:Ala,seq:tgc)
     gene            3006..4814
                     /locus_tag="VFB52_05195"
     CDS             3006..4814
                     /locus_tag="VFB52_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05761.1"
                     /translation="MVLVLVLLVPAASASAWVKEAAPALGAFGQDNSSLSVLPDGSTR
                     WIGSIGSKNPYSNQLIVRPPAGPVGLASPFPTAFGQDDSADILFLSPQDASGNQLVIR
                     EESPYASGFLAANGDPAAVAVSPIEQVIAIDLAPSGEAAALISTGSQGAVRFRPAGPS
                     GAWDSPRPLDNAGNMFSYGYAITVDPDGGIFIIYKTAQEPVLLQAYAPPGKPFGAPQQ
                     IDIDDLALNLSAYRYGASSNGHGVFAWDESTGGDTNSEELWAMTRAPGGLLGDKKLVA
                     TARAGGLVTVSSAAATDDGSGYVNFLDAGPISCLNNNYRFGGSVLAAKPGGGEWSKLN
                     TPSTGNDRMTIEAIKTEGNAVGVLASRLAYPSNICTDKDPSSSIEIQLGQGASLGPAQ
                     TIASETITGGGSSTIERPRGFAVNAGGAAAALISEPIDTANNQVHNLYYQAGVPVGPP
                     TPPDKPLPAPGKILISGKKLVVRTGKTSFKASCQRLPGEGSKLFCSVSALLAIEEKLG
                     GGKKRVARVAAAKKGKLKLKTIATAKPVKIPVGKTKEITLTLNKLGKKKLAAAKKAGL
                     SVRLKVTFKRQGYATNTLEKKLKLVAGKTKGKGGGK"
     gene            complement(4830..5594)
                     /locus_tag="VFB52_05200"
     CDS             complement(4830..5594)
                     /locus_tag="VFB52_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05762.1"
                     /translation="MSTTVADTLVLARRAVMRIVRAPDLLLAFTVQPIMFVLLFVYVL
                     GGAIETPGFDYVDFLIPGILVQTLSFGGFATALGLAEDLKKGLVDRFRSLPMSRSAVL
                     TGRTLADVATNSLSIVVMLIVGVIAGFTFDAPITNILLGIVLLLCFGYAFSWFFAFIG
                     LASSSPEAAQSLGFILLFPLSFISSAFVPIDSMPAVLQTFAEVNPFTIVVDAMRALFL
                     GTPAGNNVWGAFAWSIGIAVVFAFLSIARYKRAVVR"
     gene            complement(5591..6577)
                     /locus_tag="VFB52_05205"
     CDS             complement(5591..6577)
                     /locus_tag="VFB52_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO05763.1"
                     /translation="MSRVLAADLAIEAEGLRKSYKDVEALCGIDLSAPAGTVLGLLGP
                     NGAGKTTAVRILTTLLPPDGGSARVAGLDVVSEATELRSQIGLAGQYAAVDEHMTGAE
                     NLEMVGQLYHLPKGEPKQRAAELLERFDLAAAADRLVKTYSGGMRRRLDLAAALVARP
                     PVLFLDEPTTGLDPRSRISLWETIEGRVADGTTVLLTTQYLDEADRLADRISVIDHGQ
                     VIAEGSSDELKDQVGGERLDITLESKDEAPAAIAALEPIAAEPPAMSDGLLRMPVRER
                     KGAIAEAVRRLNDAGVGIDDIAMSRPTLDDVFLTLTGHAAEEADGEQSGEER"
     gene            complement(6624..7655)
                     /locus_tag="VFB52_05210"
     CDS             complement(6624..7655)
                     /locus_tag="VFB52_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05764.1"
                     /translation="MSILDDAIREHLELKRAHGADESELKRLEDEAFGPPQRPDEVDP
                     LAEAPTEFLTAPSEVVEEAAAEVEGEGRRVPNIADLQEAPPEPQEAPAPTESADEAEV
                     AAEASPPEPAIEEQPAAEHQLPSVEEDQQPVAEEPPTPDGVHTTEEREAIADQPTEMF
                     DVQAEIDAGAGAEAESPSDEQLLDAQTDEPRLTPVEPDLEDEDDDEFFSEQRLSEELD
                     NALEAPVTEEEPLPPLPDAPPEPEPPSEDPVPLAPVEETDEHELPPVSEAPTGHTEEA
                     TAIYDVEGEASEGEDEYEEDESGEQSVYEPETGSHDDVLEDTPEFLEDTEDDVWFEQR
                     PPKDFDFDD"
     gene            complement(7778..8932)
                     /locus_tag="VFB52_05215"
     CDS             complement(7778..8932)
                     /locus_tag="VFB52_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05765.1"
                     /translation="MVASPAAAATPDETLDQVTFIGDSVTAGFGYCGTAENAKNISCK
                     ANQEINDRWYFGDNSLDDCAPPDPPKKPTNACSNNNFNGQPWRQDPWTAGPNAPKIAY
                     PFQIAASQGKGGAAVSDWAVTGSTPADWDPNGGDFGFLLDRLKNQYVGMTLGANPLLS
                     YYTNIEFTFKTVKGRCVSSTGNWERKSFITSGSWYAGQIDDAVACLKREWARLDQTDH
                     LVDIYTSLLSRDNRVVVLGYYRSCSWSFGNWQPHANASGPAAGNDCKTQRRQVSRNNS
                     NVVTQWDQAVAVGRALNDLIQDAVGKAQDRAAQRWPGTGRDQNLVFTVPDADRWEQHQ
                     ATSPNGSWVLLNDTWIHPNRDGAGNLAETVTKAMCKRFDRWCGSPIRWKG"
BASE COUNT         1372 a         3186 c         3209 g         1379 t
ORIGIN      
        1 gccgctgtgg ggcagtgagg atcacctgcg cgggctgttc ggcgagcggg ttgggttcga
       61 gagcctggag cgcgacgtgc tcgaggtgtc cgccttcgcc cagccgggcg agttcggcga
      121 ccacttccgg cagcgctacg ggccgacgat cgtgacccgg gccaacgccg agcgcgaggg
      181 caagctggag cagctcgacc agggcatcga ccgcttctgc tcggactgga acctgggcag
      241 cgacgagaac gcgcgcttcg agctcgagta cctgctggca gtcggcaccc gcgccggcgg
      301 cgaggagcgc tagtcatggg cgcggccgcc gaaccctccg ccgaggagct gggcttccgc
      361 ctgcgcggcg ggctctacga gcccggcgac gccgagtacg aggacgcctg cacgctgttc
      421 aactcgatga tcgagcggcg gccccgcctg gtcgcccgct gcagcgcccc ggaggacgtc
      481 gtcgccgcac tcgccttcgg gcgcgagcgg gggctgccga tcgcggtgcg cggcggtggc
      541 cactcggtca ccggccgctc cctctgcgac gacggcctgg tgctcgacct gcgcgggatg
      601 cgcgacgtcg aggtcgaccc gctgcggcgc gtcgcccggg tcggcggcgg tgccacctgg
      661 gccgacgtcg acggtgcctg cggagagcac gggctggcca cgaccggcgg gcgcgtctcc
      721 accaccgggg tcgccggcct gaccatgggc ggcggctccg gctggttgga gcgcaagcac
      781 ggactcgcct gcgacaacct gctcgccgcg caactggtca ccgccggggg cgagatcgtg
      841 cgcgcggcag aggacgagaa cccggagctg ctctgggcgc tgcgcggcgg cggcgccaac
      901 ttcggcgtcg tcacggcgct ggagctggcg ctgcacccga tcgagtcgca ggtgctcggt
      961 ggcctcgtgc tgcacccggc tgagcgcggc ccggagctgc tgcgcctctt ccgcgacctg
     1021 atcgcgaacg cgcccgagga gctcgggctc gccttcgcct tcatcaccgc gcccgacgaa
     1081 gccgacgtgc cggcagagct gcgcgggcag ccggcggtga tcgtcgcggg gatgtacgcg
     1141 gggccggtgg cggagggcga agcggcgctg cgcgagctgc gcgccttcgg gcccccggcg
     1201 gccgactact tcgagccgac cacctacgcc gagttcaacc gctcgctcga cgacccgccc
     1261 ggctatcgca actactggac cgccgagcac ctcgccgagc tcaccgacga ggcgatcgag
     1321 gcgatcgccc gccgctcggc cgagatcccg gtcagcccct cgcagctctt catcgtcccc
     1381 tggggcggcg ccctgaccag ggtcgacccg accagctcac cgctcgccgg ccgccaggcc
     1441 gccttcgtcg tccaccccct gctgctctgg gaggacgagg ccgacgacgc gcgcatgatc
     1501 gccctcggcc gcgcctaccg cgacgacctc gcccccttct ccaccggcgc cgcgtacctc
     1561 aacttcttgg gcgacgaggg cgaggcccgc gtccgcgccg gcttcggcga ggagaactac
     1621 cggcggctgg ccgcggtgaa ggagcgctgg gaccccgacg gggtgttcca cggcaaccag
     1681 agcttggcgg atggaggaac gtgccctagc ccgggcgcat ctgtctgatc tgggcgaggg
     1741 cctcggcggc gttgtcgacg tcgcgctcga ccgcgcggct cagcgcggct tcggcgccac
     1801 cgagatcacc gccttgcgcc agtgagatcc cgaggttgaa ggagccgtgg ccgtcgccgc
     1861 cctcgtcgcc gcggcggtag gcggcgatcg cctcctcggg ctggcctcgt tcgtcgagga
     1921 tcgagcccag catcgaggac gccttcgcca cgccggcccg gtcaccctca cgtgccatgg
     1981 cctcggcggt ggccgggtcg ccgagctcga agcaggtgcc cgcgccgaac gaccaggctt
     2041 cgccgtgccc ccgctccgcc gatcgccgga acgcgaccac cgctcccgcc aggtcgccgc
     2101 ggccacgcag gacccaggcg agttgccagg cccctgaggc gctgccgcgc tcgtcggccc
     2161 gccgggtgcc cgcctcgaag gccgccggat cagcgcgttc ggccgccgca tcgagcatca
     2221 tgaccgcccc catcaccgac gtgtctttgt tccaaatgaa gaggtcgtgg gcgccgcaga
     2281 gccggtcggt catcgcggcg atctcctcgg cactggcgtc ctcgcggttg agcagcagcg
     2341 ccccccagtc gccgagagca cgggcactgc cgcgctcgac gcagcggaag aatgcatcct
     2401 cggcaccccg caggtccccc ttttcgcgca gcaacctgcc gaggttgccg gagccgttga
     2461 gatcgccggc ctcgtcggcc cgtcgccaga cgtgctcggc cgccgccaga tcgtggcggt
     2521 cgtcctccag cagcgccgcc agtttcagcg tgccgcgggg gtcgccggcc gcgtcggcac
     2581 gttcgaaggc cgcctgcgcc ccgtcgaggt cgcccgccga gaacagctcc tccccgcgcg
     2641 cgacatccgt gtcggctccc ccaccagttg ccattgcctg accataggag agttctcgag
     2701 gcctgcgccc tacgtgaggg tttccaagta gtggagccag ccgggatcga accggcgacc
     2761 tccgccttgc aaaggcggcg ctctgccagc tgagctatgg cccctcaccc gaaagtgtaa
     2821 gcgggaccac gacgcacggg tcaccacccc aaacaccgta cgtttccgta cgtagtagtg
     2881 acctagttcg ccgggaagtc ccaaaatcca gtagggcgga agtccagggg ttgattagcg
     2941 tcagggatat gccgaacctg agctcgctcc cgcgtccgtc caagctgcga tctgttctct
     3001 ggggggtggt cttggtactg gtgctgctgg tgccggcggc gagcgcgtcg gcttgggtga
     3061 aggaagcggc gccggcgctc ggcgctttcg gccaggacaa cagctcgctc tccgtgctgc
     3121 ccgacggcag cacccgctgg atcggctcga tcggctccaa aaacccgtat agcaaccagc
     3181 tgatcgtgcg cccgccggct gggccggtcg gtctcgcgtc cccattcccg accgccttcg
     3241 gccaagacga ctcggcggac atcctcttcc tctcccccca ggacgccagc ggcaaccagc
     3301 tggtgattcg cgaagagagc ccctacgcct ccggcttcct cgccgccaac ggcgaccccg
     3361 cggcggtcgc ggtctccccg atcgagcagg tgatcgcgat cgacctggcg ccgagcggcg
     3421 aggcggcggc gctgatcagc accggcagcc aaggggccgt gcgctttcgt cccgccggcc
     3481 cgagtggggc atgggactcg ccacggccac tcgacaacgc cggcaacatg ttctcctacg
     3541 ggtacgcgat cacggtcgac cccgacggcg gcatcttcat catctacaag accgcccagg
     3601 aaccggtctt gctgcaggcc tacgcgcccc ccgggaaacc gtttggcgcg ccccagcaga
     3661 tcgacatcga cgacctcgcc ctcaatctgt ccgcgtaccg ctacggggcg agcagcaacg
     3721 gccacggggt cttcgcctgg gacgaaagca ccggcggcga caccaactcg gaggagctct
     3781 gggcgatgac ccgcgccccc ggcggcctgc tcggcgacaa aaaactggtc gcgacggcgc
     3841 gggcgggcgg gctggtcacg gtcagctcgg cggccgcgac cgacgacggc agcggctacg
     3901 tcaacttcct cgacgccggc ccgatctcgt gcctcaacaa caactaccgc ttcggcggca
     3961 gcgtgctcgc cgcaaagccc ggaggaggcg agtggagcaa gctcaacacg ccttcgaccg
     4021 gcaacgaccg gatgacgatc gaagcgataa agaccgaggg caacgcagtt ggcgtgctcg
     4081 cctcccgcct tgcctacccg agcaacatct gcaccgacaa agacccctcg tcgtcgatcg
     4141 agatccagct cggccagggc gcctcgctcg gcccagcgca gacgatcgcc agcgagacca
     4201 tcaccggcgg cggcagctcg acgatcgagc gcccgcgcgg cttcgccgtc aacgccggcg
     4261 gtgccgcggc cgcgctgatc agcgagccga tcgacaccgc caacaaccag gtgcacaacc
     4321 tctactacca ggccggcgtg ccggtcggcc cgccgacgcc gccggacaaa ccgttgccgg
     4381 ccccgggcaa gatcctgatc agcggcaaga agctggtcgt gcgaaccggc aaaacctcct
     4441 tcaaagccag ctgccagcgg ctgcccggcg aggggtcgaa actgttctgc tcggtcagcg
     4501 cgctgctcgc gatcgaagaa aaactgggcg gcggcaaaaa acgcgtcgcc cgggtcgccg
     4561 ctgccaagaa gggcaagctg aagctgaaga cgatcgccac cgccaagccg gtgaagatcc
     4621 ccgtcggcaa gacgaaagag atcacgctga ccctgaacaa actcggcaag aaaaagctcg
     4681 cggcggcgaa aaaagcgggg ctctcggtgc ggctcaaggt gacgttcaaa cgccagggct
     4741 acgcgaccaa cacgctcgaa aagaagctga agctggtcgc cggaaaaacg aaaggcaaag
     4801 gcggcggcaa atagccgccg tcccgtccct tacctgacca cggcgcgctt gtagcgggcg
     4861 atcgagagga aggcgaagac gaccgcgatc ccgatcgacc aggcgaaggc gccccagacg
     4921 ttgttgccgg cgggggtgcc gaggaagagg gcgcgcatcg cgtcgacgac gatcgtgaat
     4981 gggttgactt cggcgaacgt ctgcagcacc gccggcatcg agtcgatcgg gacgaaggcc
     5041 gaggagatga agctgagcgg gaagagcagg atgaacccga gcgactgggc cgcctccggc
     5101 gaggacgagg cgaggccgat gaaggcgaag aaccaggaga aggcgtagcc gaagcagagc
     5161 agcaggacga tgccgagcag gatgttcgtg atcggggcgt caaaggtgaa gccggcgatc
     5221 accccgacga tcagcatcac cacgatcgag agcgagttgg tggcgacgtc ggcgagcgtg
     5281 cgcccggtga gtacggcgct gcgcgacatc ggcagcgagc ggaagcggtc gaccaggcct
     5341 ttcttcaggt cctcggcgag cccgagggcg gtcgcgaagc cgccgaagga gagggtctgg
     5401 acgaggatcc cggggatcag gaagtcgacg tagtcgaagc cgggggtctc gatcgcgccg
     5461 ccgagcacgt agacgaagag cagcacgaac atgatcggct ggacggtgaa ggcgagcagc
     5521 aggtcggggg cgcggacgat ccgcatcacc gcgcgccggg ccagcaccag ggtgtcggcg
     5581 accgtggtgc tcatcgctcc tccccgctct gctcgccgtc ggcctcctcg gcggcgtggc
     5641 cggtgagggt gaggaagacg tcgtcgaggg tcgggcggct catcgcgatg tcgtcgatcc
     5701 cgaccccggc gtcgttgagg cggcggaccg cctcggcgat cgcgcccttg cgctcccgca
     5761 ccggcatccg cagcaggccg tcgctcatcg ccggcggctc ggcggcgatc ggttcgagcg
     5821 ccgcgatcgc ggccggcgct tcgtccttgg actcgagcgt gatgtcgagg cgctcgccgc
     5881 cgacctggtc cttgagctcg tcggagctgc cttcggcgat cacctggccg tggtcgatca
     5941 ccgagatgcg gtcggcgagc cggtcggcct cgtcgaggta ctgggtggtg agcagcaccg
     6001 tggtgccgtc ggcgacccgc ccctcgatcg tctcccagag gctgatccgg ctgcgcgggt
     6061 cgaggccggt ggtcggctcg tcgaggaaga ggacgggcgg gcgggcgacc agggcggcgg
     6121 cgaggtcgag ccggcggcgc atcccgcccg aataggtttt gaccaggcgg tcggcggcgg
     6181 ccgcgaggtc gaagcgctcg agcagctcgg cggcgcgctg cttcggctcg cccttcggca
     6241 ggtggtagag ctggccgacc atctccaggt tctcggcccc ggtcatgtgt tcgtcgaccg
     6301 ccgcgtactg cccggcgagc ccgatctggc tgcgcagctc ggtcgcttcg ctgaccacgt
     6361 cgagcccggc gacccgggcg ctgccgccgt cgggcgggag cagggtggtg aggatgcgaa
     6421 ccgcggtcgt cttgccggcg ccgttggggc cgagcaggcc gagcacggtt ccggcggggg
     6481 cgctgaggtc gattccgcag agggcctcga cgtccttgta ggacttgcgc aggccttcgg
     6541 cttcgattgc gaggtcggcg gccaagacgc ggctcaacct accgagtatt caggagcggc
     6601 gcaatcacgc gccgcggctc cggtcaatcg tcgaagtcga agtccttcgg cgggcgctgc
     6661 tcgaaccaga cgtcgtcctc ggtgtcctcg aggaactcgg gggtgtcttc gagcacgtcg
     6721 tcgtggcttc ccgtctcggg ttcgtagacg ctctgctcgc cggactcgtc ctcctcgtac
     6781 tcgtcctcgc cctcgctcgc ctcgccctcg acgtcgtaga tcgcggtcgc ttcttcggtg
     6841 tgaccggtgg gggcctcgct caccggtggg agctcgtgct cgtcggtctc ctcgaccggg
     6901 gcgagcggga ccgggtcctc gctgggcggc tcgggctcag gcggagcgtc gggcagcggc
     6961 ggaagcggct cctcttcggt gacgggggcc tcgagcgcgt tgtccagctc ctcggagagg
     7021 cgctgttcgg agaagaattc gtcgtcgtcc tcgtcctcga ggtcgggctc gaccggggtg
     7081 aggcgcggct cgtcggtctg cgcgtcgagc agctgctcgt cggaggggct ctccgcctcg
     7141 gcgccggcgc cggcgtcgat ctcggcctgc acgtcgaaca tttcggtcgg ctggtcggcg
     7201 atcgcctcgc gctcttcggt ggtgtggacg ccgtcgggcg tgggcggctc ctcagccacc
     7261 ggctgctggt cctcctccac ggagggaagc tggtgctcgg cggcgggctg ctcctcgatc
     7321 gcgggctcgg gcggcgacgc ctcggcggcg acctcagcct cgtcagccga ctcagtcggt
     7381 gcgggagcct cctgcggctc cggcggcgcc tcctgcaggt cggcgatgtt gggcacgcgc
     7441 cgtccctcgc cctcgacctc ggcagcggcc tcctcgacca cctcggaagg agccgtcaag
     7501 aactcggtcg gcgcctcggc cagcggatcg acctcgtcgg gtcgctgcgg cggcccaaac
     7561 gcctcgtcct cgagtcgttt cagctcggac tcatcagcgc catgcgcgcg cttcagttcc
     7621 agatgctctc gaattgcgtc gtcaagaatg ctcatgtggc ccaccgccgg aacaggctgc
     7681 caaagactat gcggttattg ggcgctctgg gagtgaatgg gagcaacttc ccacccgccg
     7741 cgggccggcg acgcgcacac gccgccggcc cgcggaccta gcccttccag cgaatcggcg
     7801 agccgcacca gcggtcgaag cgcttgcaca tcgccttggt gacggtttcg gccaggttcc
     7861 cggccccgtc gcggttgggg tggatccagg tgtcgttgag cagcacccag gaaccgttgg
     7921 ggctggtcgc ctggtgctgt tcccagcggt cggcgtccgg aaccgtgaag accaggttct
     7981 ggtcgcggcc ggtccccggc catctctgcg ccgcgcggtc ctgggccttg ccgacggcgt
     8041 cctggatcag gtcgttgagc gcccggccga cggccaccgc ctggtcccac tgggtgacca
     8101 cgttcgagtt gttgcggctg acctggcggc gctgggtttt gcagtcgttg ccggcggccg
     8161 gccccgaggc gttggcgtgg ggctgccagt tgccgaacga ccaggagcag ctgcggtagt
     8221 agccgagcac gaccacccgg ttgtcgcgcg agagcagcga ggtgtagatg tcgaccaggt
     8281 gatcggtctg gtcgagccgc gcccattcgc gcttcaggca ggcgaccgcg tcgtcgatct
     8341 ggccggcgta ccagctgccg gaggtgatga acgacttccg ttcccagttg ccggtcgagg
     8401 agacgcagcg gcccttcacc gttttgaagg tgaactcgat gttcgtgtag taggagagca
     8461 acgggttggc gccgagcgtc atccccacgt actggttctt cagccggtcg agcaggaagc
     8521 cgaagtcacc gccgttcggg tcccagtcgg ccggcgtcga accggtcacc gcccaatcgg
     8581 aaacggccgc gccgcccttg ccctggctgg ccgcgatctg gaacggatac gcgatcttcg
     8641 gcgcgttcgg gcccgcggtc caggggtcct ggcgccacgg ctggccgttg aagttgttgt
     8701 tgctgcacgc gttggtcggc ttcttgggag gatcaggcgg cgcgcagtca tcgaggctgt
     8761 tgtccccgaa gtaccagcga tcgttgattt cctggttcgc cttgcagctg atgttcttgg
     8821 cgttctcagc ggttccgcag tagccgaagc cggcggtcac cgagtcgccg ataaaggtga
     8881 cctggtcgag cgtttcgtca ggcgtcgccg cggccgccgg agatgcgacc aaagcaagcg
     8941 ctgcgagtag agatccaaag acgactgcga tccgcaagcc gcccaggggc cgagactggg
     9001 aatgcatcgg agaatccttc cggtgtcagg ttggggggat ttccctgccg aaccgaatcg
     9061 acaaagaacc aacgtgttcg gcacccccac cccccgactt aagtccccac cccagatctc
     9121 agcgcttctc cgtcgccccg cccccc
//
LOCUS       DASRIG010000190         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1146738, whole genome shotgun sequence.
ACCESSION   DASRIG010000190 DASRIG010000000
VERSION     DASRIG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146738"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFB52_05220"
     CDS             <1..336
                     /locus_tag="VFB52_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05766.1"
                     /translation="HWPGNIRELEHLVERAVLTAESEVLKITLPPPTRGAVVPLESAT
                     HQTPPASENGTQLVTLAAHDRAYLERVLRHTHGRVSGPRGAAAILGLKPSTLRSRMKK
                     LGLSLPPEE"
     gene            461..3817
                     /locus_tag="VFB52_05225"
     CDS             461..3817
                     /locus_tag="VFB52_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZO05767.1"
                     /translation="MPKTMFPSFSMLCLVLLACLGGRAPAQTHGEITGVITDASGATV
                     PDVKVTVTNLATGAARLAQTNDAGVYSFPALFPGSYSLRVEKSGFRAIKQDGVELQVQ
                     QTARHDFTLEVGDVEQTVDVSGGATLLTTESTTVGTVIENKRIVDLPLNGRNFLQLVA
                     TAPNVSFGFQQAGQSGSRQGGTRANQQISVAGQRSYFNRFTIDGIENTDVNFNTPIIL
                     PSIDALQEFKVQTGVFPAEFGRASTQINVSTKSGTREYHGTVFEFLRNDVFDARIYDF
                     TGKRAPDSPKEPLRWNQYGFTLGGPVWLPKEVFGPIGYDNRERVFFLTNFEGFRDRRT
                     SRGRYQLPPAAWRTGDFSSLSNPIFDPLTGRQFPGNIIPQSRIHPTSLQLLEFYPSPN
                     LAPASLGINHEQPQGRKIDKDQFIGRGDFIESTSSSWAGRYSWGDEVQLDPGLQLNGT
                     KLLTTVKQVMASNTRTFSANKVNEVRFGYNSFFNSLGRELAGVRDVVSELDIPGVNPG
                     PPISWGIPSIAVQGFSGFGDDTEGPYVNTNSTYQLVDNFSWTVGSHSFRFGGELRWDH
                     YNQVGNQFARGGFLFEATATSNLGAAATGNGFADFLLGYCKRCEVSVSLADAQFRAFS
                     QYYYFDDTWKLTPKLTLNLGLRYEYTPPWFDKTGRLVNIYVPFADRTPNVADLSRHPV
                     FVRMGSGDFYEGLSLRFNPAIQVARDGRLGERLVDFDRNDFAPRFGLAWSPTSRWTVR
                     LGGGVFYSQDTGNPRFDMARNLAGRRRDESTPTQLDLTWDAPFRNLGGTVQVNNPYVL
                     GNIFARRTPYVIQYMLNVQRELGGNLLVEIGYLGSVARKLESLRAFNESIPGATGSVL
                     SRAPYPEFGRIQEVDGSGKSSYNGLSFKVEKRFSAGLSFLAGYTWSRSIDNASAIRSH
                     NGDTLFPQNSYNLAAEKALSSFHTSHRFVTSGTYELPFGDGKRWLDRGGFFNLLLGGW
                     QVSPIVTVQTGFPLTVVSGRDQSNTGAGFDRPNVVSGQETHLPRGERSLDRWFNTAAY
                     VLQPFGTHGTAGRNTLISPGFVQVDAALLKNFRIKEAVNVQFRFEAFNAPNHPNWGNP
                     DTSVTSANYGRITGMRGLMRELQFGLKLLF"
     gene            3921..4682
                     /locus_tag="VFB52_05230"
     CDS             3921..4682
                     /locus_tag="VFB52_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZO05768.1"
                     /translation="MKKLIARRRVISGPVLAALLVPAVVAYQAARPRSSPADPLIAGF
                     QKTTVASVSDAVDQVVGRRGYMSHEMRPMIAGQVVGRAATAVVRPAPPEQSTPALAVK
                     HSVEMIDSAKEGEVGVIVVEQGLDVAAIGGLMGTAAKARGMAGMILDGGVRDLAELRA
                     LGLPTYARSVVPSTAVGRFASAAKQVPVECAGVTVKPGDIIVAGEDGVVCVPQERAAE
                     VLKRAQEIDEREIKMVPFIKQQKSLQKVVEMFNRI"
BASE COUNT          799 a         1755 c         1545 g          748 t
ORIGIN      
        1 cactggccgg gtaacatccg cgagctggag cacctggtcg agcgcgccgt gctcacggcc
       61 gagagcgagg tccttaagat caccctcccc ccgccgaccc gcggcgcggt cgtcccgctc
      121 gagtcggcca cccaccagac tccgccggcc tccgagaacg gcacgcagtt ggtcactctc
      181 gccgcccacg accgcgccta cctcgagcgc gtcctgcgcc acacccacgg ccgcgtctcc
      241 ggcccgcgcg gggccgccgc gatcctcggc ctcaaaccct cgaccctccg cagccggatg
      301 aagaagctcg gcctcagtct gccgcccgag gagtaagatc gcccccgctt ggcattcagg
      361 ccttcttcag atatatttac tgacctcgcg cagcaccgta tcacccaatc agcgcgggga
      421 gtttttcatc ccaaccagac cccagatcaa ggagccgcag atgccaaaga ccatgttccc
      481 cagcttcagc atgctttgtc tcgtcctcct cgcctgcctc ggcgggcgcg ccccggcaca
      541 gacccacggc gagatcacgg gcgtgatcac cgacgcgagc ggcgcgacgg tgcccgacgt
      601 caaggtgacg gtcaccaatc tcgccacggg cgccgcgcgc ctggcgcaga ccaacgacgc
      661 gggtgtttac agtttcccgg ccctcttccc cgggagttac agcctccgcg tcgagaagtc
      721 gggctttcgg gcgatcaagc aggacggggt cgagctccag gtccagcaga cggcccgcca
      781 cgacttcacg ctcgaggtcg gcgacgtcga acagacggtc gacgtctcgg gcggggcgac
      841 gctgctcacg accgagagca cgacggtcgg gacggtcatc gagaacaagc ggatcgtcga
      901 cctgccgctc aacgggcgca acttcctcca gctcgtggcc accgccccga acgtgagctt
      961 cggcttccag caggccgggc agtccggctc gcgccagggc gggacgcggg ccaaccagca
     1021 gatctcggtc gccgggcagc ggagctactt caaccgcttc accatcgacg ggatcgagaa
     1081 caccgacgtc aacttcaaca ccccgatcat cctcccctcg atcgacgccc tccaggagtt
     1141 caaggtccag accggggtct tcccggccga gttcggccgc gcctcgaccc agatcaacgt
     1201 ctcgaccaag tcggggacgc gcgagtacca cgggacggtc ttcgagtttc tgcgcaacga
     1261 cgtcttcgac gcgcgcatct acgacttcac gggcaagcgc gccccggaca gcccgaagga
     1321 accgctcagg tggaaccagt acggcttcac cctcggcggc cccgtctggc tgccgaagga
     1381 ggtcttcggc cccatcggct acgacaaccg cgagcgggtc ttcttcctga ctaacttcga
     1441 ggggttccgc gaccgccgga cgtctcgcgg gcgctaccag ctgccgccgg cggcctggcg
     1501 cacgggcgac ttctcctcgc tctcgaaccc gatcttcgac ccgctcacgg ggcggcagtt
     1561 ccccggcaac atcatcccgc agagccggat tcacccgacc tcgctccagc tcctcgagtt
     1621 ctacccgtcg cccaacctgg cgcccgcctc gctcggcatc aaccacgagc agccgcaggg
     1681 gcggaagatc gacaaggatc agttcatcgg ccgcggcgac ttcatcgaga gcacctcgtc
     1741 gagctgggcc gggcgctaca gctggggcga cgaggtccag ctcgaccccg ggctgcagct
     1801 caacgggacg aagctgctga cgacggtcaa gcaggtcatg gcctctaaca cgcggacctt
     1861 cagcgcgaac aaagtcaacg aggtgcgctt cggttacaac tcattcttca acagcctcgg
     1921 gcgcgaactg gccggggtgc gcgacgtcgt cagcgagctg gacatccccg gcgtcaatcc
     1981 cgggccgccg atctcgtggg gcatcccgag catcgccgtc cagggcttct cgggcttcgg
     2041 cgacgacacg gaggggccct acgtcaacac caacagcacc taccagctcg tcgacaactt
     2101 cagctggacg gtcggcagcc acagcttccg cttcggcggc gagctgcgct gggaccacta
     2161 caaccaggtc ggcaaccagt tcgcgcgcgg gggcttcctc ttcgaggcga cggcgacctc
     2221 caacctcggg gccgcggcga ccggcaacgg cttcgccgac ttcctgctcg gctactgcaa
     2281 gcgctgcgag gtctcggtct cgctcgccga cgcgcagttc cgcgccttca gccaatacta
     2341 ctacttcgac gacacctgga agctgacgcc gaagctgacg ctcaacctcg ggctgcgcta
     2401 cgagtacacc ccgccctggt tcgacaagac cgggcggctg gtcaacatct acgtgccatt
     2461 cgccgaccgg acgccgaacg tcgccgacct gagccgccac ccggtcttcg tccgcatggg
     2521 cagcggcgac ttctacgagg ggctgtcgct gcgcttcaac ccggcgatcc aggtggcgcg
     2581 cgacgggcgg ttgggggagc ggctggtcga ttttgaccgc aacgacttcg cgccgcgctt
     2641 cggcctcgcc tggtcaccga cgagccggtg gacggtgcgc cttggcggcg gcgtcttcta
     2701 ctcgcaggac acgggcaacc cgcgcttcga catggcgcgc aacctggccg ggcggcggcg
     2761 cgacgagtcg accccgaccc agctcgacct cacctgggac gcgccgttcc gcaacctcgg
     2821 cggcacggtc caggtcaaca acccctatgt cctcggcaac atcttcgccc gccgcacgcc
     2881 gtacgtcatt cagtacatgc tcaacgtgca gcgcgagctc ggcggcaacc tcctggtcga
     2941 gattggctac ctcgggtcgg tcgcgcgcaa gctcgagtcg ctgcgcgctt tcaacgagtc
     3001 gatcccgggg gcgaccggct cggtcctctc ccgcgcgccc taccccgagt tcgggcgcat
     3061 ccaggaggtg gacggcagcg gcaagtcgtc ctacaacggc ctgagcttca aggtggagaa
     3121 gcgcttcagc gccgggctga gcttcctcgc cggctacacc tggtcgcggt cgatcgacaa
     3181 cgcgagcgcg atccggagcc acaacggcga cacgcttttc ccgcagaaca gctataacct
     3241 cgcggccgag aaggcgctct cgagcttcca cacgtcgcac cgcttcgtta cctcggggac
     3301 ctacgagctg cccttcggcg acgggaagcg ctggctcgac cggggcggct tcttcaacct
     3361 cctgctcggc ggctggcagg tcagcccgat cgtgaccgtc cagacgggct tcccgctaac
     3421 ggtcgtctcc gggcgggacc agtcgaatac cggcgccggg ttcgaccgac cgaacgtcgt
     3481 ctcggggcag gagacccacc tgccgcgtgg cgagcgatcg ctcgaccggt ggttcaacac
     3541 ggccgcctac gtgctccagc cgttcggcac tcatggcacc gccggccgga acacgctcat
     3601 cagccccggc ttcgtccagg tggacgcggc gctgctcaag aacttccgga tcaaggaggc
     3661 cgtcaacgtg cagttccgct tcgaggcctt taacgcgccg aaccacccca actgggggaa
     3721 cccggacacc tcggtcacaa gcgcgaatta cggccgcatc accggcatgc gcggcctcat
     3781 gcgcgagctg cagttcgggc tcaagctgct cttttagcaa acctgtaggg gcagggctcg
     3841 tccctgcccg cggtccgcta ccgcgaggct gggcaggacg agtgctgccc ctacacgtct
     3901 ccgatcaagg aacgaagttt atgaagaagc tcatcgctcg ccgtcgtgtc atctcgggcc
     3961 cggtgctcgc ggcgctgctc gtgccggcgg tggtcgccta ccaggccgcc agaccgcggt
     4021 cgtccccggc cgacccgctg atcgccggtt ttcagaagac gacggtcgcc tcggtctccg
     4081 acgcggtcga ccaggtggtc ggccggcgcg gctatatgtc gcacgagatg cgccccatga
     4141 tcgcgggcca ggtggtcggc cgcgccgcca cggcggtcgt ccggcccgcg ccgcccgagc
     4201 aatcgacgcc cgcgctcgcc gtcaaacact cggtcgagat gatcgacagc gccaaggaag
     4261 gcgaggtcgg cgtgatcgtc gtcgagcagg ggctcgacgt ggcggcgatc ggcggcctca
     4321 tgggcacggc ggccaaggcg cgtgggatgg ccgggatgat cctcgacggc ggcgtccgcg
     4381 acctggccga gctgcgcgcc ctgggcctcc cgacctacgc gcgctcggtc gttccctcga
     4441 ctgccgtcgg gaggttcgcg agcgccgcca agcaggtccc ggtcgagtgc gctggcgtca
     4501 cggtcaagcc cggcgacatc atcgtcgcgg gcgaggacgg cgtcgtctgc gtcccgcagg
     4561 agcgggcggc cgaggtgctc aagcgggcgc aggagatcga cgagcgcgag atcaagatgg
     4621 tccccttcat caagcagcag aagtcgctgc aaaaagtcgt cgagatgttc aaccggattt
     4681 gagccgggcg cgtctactcg ctgatgacgg ccggcgggat cggtttgccg acgacgcccc
     4741 ggtactcccg gtaatcgatc ccgagcaggc tgaggatcgt cggcgcgatg tcgcgctgga
     4801 agaaggtggg tgtgttcagc gcctcgcccg tcgccggcgt gtcgggc
//
LOCUS       DASRIG010000191         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_91617, whole genome shotgun sequence.
ACCESSION   DASRIG010000191 DASRIG010000000
VERSION     DASRIG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91617"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFB52_05235"
     CDS             <1..1182
                     /locus_tag="VFB52_05235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZO05769.1"
                     /translation="FLGGPPAAAIGVLTIAAGWLRWRDNAHDLLVNVLTYATFPLVAG
                     LAFHEAVVRGDVGNTDPMFFVLVFATFLLALAINFSMVAADASIVFGASFTEKVRTAL
                     IPLLPSELAAAMMTVGVAFIYVQIGIAGVALFGIVLVTFQYLLGALLLSQQRAEELEL
                     RSKQLASFQVGMLSAMLRTLDLRDQMTARHSAAVARYSRAIAQRAGYSRREEELVHIA
                     ALLHDIGKFILPDRILKANVPLTDEDWMLIKRHPQQGARVVSSLDGYGPVAEIILAHH
                     ERIDGKGYPRGLAGDEIPELSRIISVADTYDVMTARDSYRTPMSSQDAIEELRRVAGK
                     QLDAKFVEVFIELLEGKDVSFQHSEAADFEKELALESRIAETASPDGPQSRFQVAGVS
                     V"
     gene            1216..2247
                     /locus_tag="VFB52_05240"
     CDS             1216..2247
                     /locus_tag="VFB52_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO05770.1"
                     /translation="MRAVTFQGPEQVRVEEKPEPELVAADDAVVRIEATGICGSDLHI
                     YHGRFPVEPGFTIGHEFVGTVIAAGEEVERVAVGDRVLGCFHTACATCSPCLRGDYHK
                     CRKQRTFGHGTALGDLQGAQAEQLLVPRANLTLRRVPEGMSSDVALFAGDVMGTGFHS
                     IVNAGMRSGDTVAVFGLGPVGLCAVQSALAGGAVEVFAVDSVEERLQMAASFGATPVH
                     LTEQEPKKLIRAATDGGVDLAVDAVGDPGPLAMAIGLTRDAGTVSGIGAYQGRAEIPL
                     GLAWVKSLNLCLGLANVVAHVDRVLALMAAGRLDPAPLVSSHMSLDEASEAYAIYDNR
                     EALKIVLTP"
     gene            complement(2268..2624)
                     /locus_tag="VFB52_05245"
     CDS             complement(2268..2624)
                     /locus_tag="VFB52_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05771.1"
                     /translation="MELMSWTDGKLEERLDRIDERFEQVNGRFEQVNERFEQVNERFA
                     QVDRRLGEVVQESNRRFDAVDRRFDEASEESKQIRQDVREISQVLRDLQMLLTRAALG
                     VVFIFIAAVVAMIFNG"
     gene            complement(2718..3521)
                     /locus_tag="VFB52_05250"
     CDS             complement(2718..3521)
                     /locus_tag="VFB52_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZO05772.1"
                     /translation="MGALSGQSRPVLWQIDISHYSEKVRWALDYKEVDHVRRSPFPGM
                     HMAIALTLTGGKAKTFPILQLDGRTIPDSTAAIAALEEYEPDPALYPSDPEERRRALA
                     LEDYFDEQLGPHARLLPFHELLSDPDLFAELAAQSVPAPMAKAKGLVGGYARAYTRLR
                     WGADNAQAALEARAGIVAAMDRLEAELLNNGGEFLVGESFSVADLTAASLFYPVVGPE
                     GGPIPPGQPMPETFERFRENLSERPGFTWVEETFRHHRHRSGRFARAGR"
     gene            3547..3825
                     /locus_tag="VFB52_05255"
     CDS             3547..3825
                     /locus_tag="VFB52_05255"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZO05773.1"
                     /translation="MALLADSEIDARLAGRPGWRRAGETIEKVFKCGDFVGSVAFVDK
                     LVAPAEGMNHHPDLEISWDKVKVSLSTHSEGGLTAADFELAAQIDALA"
     gene            3848..4198
                     /locus_tag="VFB52_05260"
     CDS             3848..4198
                     /locus_tag="VFB52_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05774.1"
                     /translation="MAKRKKQRRPREKAATVDYTDPEGNVLSLRESLSAGTIRKLGEP
                     PSSAAASLDDAWQRRSELLFERLAVNWEIAGLPLDDQKMLLGRYRMADAETQRWVRQT
                     IADHLQRYIPELAG"
     gene            4225..4641
                     /gene="arfB"
                     /locus_tag="VFB52_05265"
     CDS             4225..4641
                     /gene="arfB"
                     /locus_tag="VFB52_05265"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZO05775.1"
                     /translation="MPDELRIDDRLAIPLTEIELRTSRSSGPGGQHANVTASRVEAVF
                     EVEASAALDEAQRARLRERLGPTVTAVAQDARGQARNRELALARLAEKIAAALRVPRR
                     RRPTRPTKASRKRRLEQKRRAGERKRGRRRFGYGDE"
     gene            4677..5057
                     /locus_tag="VFB52_05270"
     CDS             4677..5057
                     /locus_tag="VFB52_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05776.1"
                     /translation="MLGKSKAFSGYAVDDIDAAREFYAQTLGLETNEPMEGMLLSLEL
                     DGGQRPTLIYAKPDFEPATYTVLNFPVDDVDAAVDGLAARGVKFERYEGMDQDEKGIS
                     RGAGPQIAWFKDPAGNILSVLKEQ"
     gene            complement(5069..5488)
                     /locus_tag="VFB52_05275"
     CDS             complement(5069..5488)
                     /locus_tag="VFB52_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO05777.1"
                     /translation="MTEPSLSFTSIDPDAGYFHLLRRELGVTSFGINQLILQPHQRLR
                     VHRHERQEEVYLVLEGELTLIVEGKPHVLAVGDLARVGPELKRQLTNPGATKTIVLAL
                     GGDGDHQSRDGIAWSGWDDEGEGRPPKDVPLPDDLPR"
     gene            complement(5485..>5943)
                     /locus_tag="VFB52_05280"
     CDS             complement(5485..>5943)
                     /locus_tag="VFB52_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO05778.1"
                     /translation="EPARFAPFAEMHREAAREAGHQPPALSINSHGFVATDSQVAADT
                     AYPPFALMMDRIGRERGWPPMARQTFEASRTLRGANFVGNPEEVAAKIVHQHAIFGHQ
                     RFLIQFSVGTLPHADVMRSIELFGTEVAPLVRHELGDAEDGAVGENVAAR"
BASE COUNT          895 a         2044 c         2084 g          920 t
ORIGIN      
        1 ttcctcggcg gacccccggc cgcggcaatc ggcgtgctga cgatcgccgc gggctggttg
       61 cgctggcgcg acaacgccca cgacctactg gtcaacgtgc tgacctacgc gaccttcccg
      121 ctcgtcgccg gcctcgcctt ccacgaagcg gtcgtccggg gtgacgtcgg caacaccgac
      181 ccgatgttct tcgtgctcgt cttcgcgacc ttcctgctgg cgctcgcgat caacttttcg
      241 atggtcgccg ctgacgccag catcgtcttc ggcgcctcgt tcaccgaaaa agttcgcacc
      301 gccctgatcc cgctgctgcc ctcggagctc gccgcagcga tgatgacggt cggcgtcgcc
      361 tttatctacg tccagatcgg gattgccggt gtggcgctgt tcggcatcgt cctggtcacc
      421 ttccagtacc tgctcggcgc tttgctgctc tcccagcagc gggccgagga gctggagctg
      481 cgctccaagc agctggccag cttccaggtc gggatgttga gcgcgatgct gcgcaccctc
      541 gatctgcgcg atcagatgac cgctcgccac tcggccgcgg tcgcccgcta ctcgcgcgcg
      601 atcgcccagc gggccggcta ctcgcgccgc gaggaggagc tggtccacat cgccgcgctg
      661 ctccacgaca tcggcaagtt catccttccc gaccgcatcc tcaaggccaa cgtgccgctc
      721 accgacgagg actggatgct gatcaagcgc cacccgcagc agggcgcgcg cgtggtctcc
      781 tcgctcgacg gttacgggcc ggtggcggag atcatcctcg cccaccacga gcggatcgac
      841 ggcaagggct acccgcgcgg gctggccggc gacgaaatcc ccgagctctc gcggatcatc
      901 tcggttgccg acacctacga cgtgatgacc gcacgcgact cctaccgcac gccgatgtcg
      961 agccaggacg cgatcgagga gctgcgccgg gtcgccggca aacagctcga cgccaaattc
     1021 gtcgaggtct tcatcgagct gctcgagggc aaggacgtct ccttccagca cagcgaggcg
     1081 gccgacttcg agaaagagct ggcgctggaa tcgcggattg ccgagaccgc cagccccgac
     1141 ggcccgcaga gccgcttcca agtcgccggc gtcagcgtct agccagaccc ctaaccgccc
     1201 gctacggttg cggcgatgcg tgccgtcacc tttcagggtc ccgagcaggt ccgggtcgag
     1261 gagaagcccg agcctgagct cgtcgccgcc gacgacgcgg tcgtccgcat cgaggcgacc
     1321 gggatctgcg gctcggacct ccacatctac cacgggcgct tcccggtcga gccgggcttc
     1381 acgatcggcc acgagttcgt cggcacggtg atcgcggccg gtgaggaggt cgagcgggtc
     1441 gcggtcggcg accgcgtgct cggctgcttc cacaccgcct gcgcgacctg ctcgccctgc
     1501 ctgcgcggcg attaccacaa gtgccgcaag cagcggacct tcgggcacgg caccgccctc
     1561 ggcgacctgc agggcgccca ggcggagcag ctgctggtcc cgcgcgcgaa cctgaccctg
     1621 cgccgggtgc cggaggggat gagctccgac gtcgccctgt tcgccggcga cgtgatgggt
     1681 accggcttcc actcgatcgt caacgcgggg atgcgctccg gcgacacggt cgccgtgttc
     1741 ggactcgggc cggtcggcct ctgcgcggtg cagtcggcgc tggccggcgg cgcggtcgag
     1801 gtcttcgcgg tcgacagcgt cgaggagcgg ctgcagatgg ccgcctcgtt cggggcgacc
     1861 ccggtgcacc tgaccgagca ggagccgaag aagctgatcc gggcggcgac cgacggcggc
     1921 gtcgacctcg cggtcgacgc ggtcggcgat cccggtccgc tggcgatggc gatcggcctg
     1981 acccgcgacg ccggcaccgt ctccgggatc ggcgcctacc agggccgggc ggagatcccg
     2041 ctcggcctcg cctgggtgaa gagcctcaac ctctgcctcg gcctcgccaa cgtggtcgcc
     2101 cacgtcgacc gcgtgctcgc gctgatggca gccggccgcc tcgaccccgc cccgctggtc
     2161 agcagccaca tgtcgctcga cgaggcgagt gaggcctacg cgatctacga caaccgcgaa
     2221 gccctgaaga tcgtcctcac tccgtagcgc cgggccggcc cgcgaggtca gccgttgaag
     2281 atcatcgcga ccacggccgc gatgaagata aagacaaccc cgagcgcggc ccgcgtcagc
     2341 agcatctgga gatcgcggag cacttggctg atttctcgca cgtcctgcct gatctgcttg
     2401 ctttcttcgc tggcttcgtc gaaccgccgg tcgacggcgt cgaaccgccg attgctttct
     2461 tggacgactt cacctagacg ccgatccacc tgcgcgaacc gttcgttgac ttgctcgaac
     2521 cgttcgttga cctgctcgaa ccgcccgttg acctgctcga acctctcgtc gattcggtcg
     2581 aggcgctctt caagttttcc gtcggtccag ctcatcagct ccatgatggg accttaggcg
     2641 gaatcatgca acgtgttgtc tgcagatcgc aatttctccg taacaaaaga ccggaggctt
     2701 gagagccagc gggcgaccta gcggccggcg cgggcgaagc ggccgctgcg gtgccggtgg
     2761 tggcggaagg tctcctcgac ccaggtgaag ccggggcgct cggagaggtt ttcgcggaag
     2821 cgctcgaagg tctccggcat cggctggccg ggcgggatcg ggccgccctc ggggccgacc
     2881 acggggtaga agagcgaggc cgcggtgagg tcggcgacgc tgaagctttc tccgaccagg
     2941 aactcgccgc cgttgttcag cagctccgcc tccagccggt ccatcgcggc gacgatcccg
     3001 gcgcgggctt cgagcgcggc ctgggcgttg tcggcccccc agcgcaggcg ggtgtaggcg
     3061 cgggcgtaac cgccgaccag ccccttcgct ttggccatcg gtgctggcac cgactgcgca
     3121 gcgagctcgg cgaagaggtc gggatcgctg agcagttcgt ggaaggggag caggcgggcg
     3181 tgcgggccga gctgctcgtc gaagtagtcc tcgagcgcca gcgcgcgacg gcgctcctcc
     3241 ggatcgctcg ggtagagcgc cgggtctggt tcgtactcct cgagcgctgc gatcgccgcg
     3301 gtcgagtcgg ggatcgtgcg gccgtcgagc tgcaggatcg ggaaggtttt cgccttgccg
     3361 ccggtcaggg tcagcgcgat cgccatgtgc atgccgggaa acggcgagcg gcgcacgtgg
     3421 tcgacttcct tgtagtcgag ggcccagcgg accttctcgc tgtagtgcga gatgtcgatc
     3481 tgccaaagga ccggccgtga ctgtccggat agcgccccca tcgcctggtt gagagaatat
     3541 gcgctaatgg cccttctcgc tgactcggag atcgatgcgc gcctcgccgg gcgtcccggc
     3601 tggcgccgcg ccggcgagac gatcgagaag gtcttcaagt gcggtgactt cgtcggctcg
     3661 gtcgctttcg tcgacaagct ggtcgcgccg gccgagggga tgaaccacca tccggacctc
     3721 gagatctcgt gggacaaggt caaggtctcg ctctcgaccc actccgaagg cggcctcacc
     3781 gccgccgact tcgagctcgc cgcgcagatc gacgcgctgg cttaggccgc tgtcaaactc
     3841 ggcggcgatg gcaaagcgca agaagcagcg gcgcccgcgc gagaaagcgg cgacggtcga
     3901 ctacaccgat ccggagggca acgtgctgag cctgcgtgaa tcgctcagcg ccggcacgat
     3961 ccgcaagctc ggcgagccgc cgagcagcgc cgccgcctcg ctcgacgacg cctggcagcg
     4021 gcgctcggag ctgttgttcg agcggctggc ggtcaactgg gagatcgctg ggctgccgct
     4081 cgatgaccag aagatgctgc tcggccgcta ccggatggct gacgccgaga cgcagcgctg
     4141 ggtgcggcag acgatcgccg accatctcca gcgctacatc cccgagctgg cggggtaggc
     4201 ggccgcgcgg ggtaccttcc acccgtgccc gacgagttgc gcatcgacga tcgcctcgcg
     4261 atcccgctga ccgagatcga gttaaggacc agccgctcct ccggccccgg cggccagcac
     4321 gccaacgtga ccgcttcgcg cgtggaagcg gtcttcgagg tcgaggcctc ggcggcgctc
     4381 gacgaggcgc agcgggcgcg gctgcgggag cggctcggcc cgacggtgac cgcggtcgcc
     4441 caggacgcgc gcggccaggc gcgcaaccgc gagctggcgc tggcgcggct ggcggagaag
     4501 atcgcagcgg cgctgcgggt gccgcgtcgc cgccgcccga cccggccgac caaggcctcg
     4561 cgcaagcgcc ggctcgagca gaagcgccgc gccggcgagc gcaagcgtgg acgccgccgc
     4621 ttcgggtacg gggacgagtg acgcgaacag cgatcaacca gggaagggag aacgagatgc
     4681 tgggcaagag caaggccttc agcggctacg ccgtcgacga catcgacgcg gcgcgcgagt
     4741 tctacgcgca gacgctgggc ctggagacga acgaaccgat ggagggcatg ctgctctcgc
     4801 tcgaactcga cggcggacag cggccgacgc tgatctacgc caaacccgac ttcgagcccg
     4861 ccacctacac cgtgctcaac ttcccggtcg acgacgtcga cgcggcggtc gacgggctcg
     4921 ctgcccgcgg cgtcaagttc gagcgctacg agggcatgga ccaggacgag aaaggcatct
     4981 cccgcggcgc cggcccccag atcgcctggt tcaaggaccc ggccggcaac atcctctcgg
     5041 tgctgaagga gcagtaaaag gcgcgcggct agcgcggcag gtcgtcggga agcgggacgt
     5101 ccttgggcgg gcgtccctcg ccctcgtcgt cccatcctga ccaggcgatg ccgtcgcggc
     5161 tctggtgatc gccgtcgccg ccgagggcga ggacgatcgt cttggtggcg cccgggttgg
     5221 tcagctggcg tttgagctcc gggccgacgc gggcgaggtc gccgaccgcg agcacgtgcg
     5281 gcttgccttc gacgatcagg gtcagctcgc cctcgagcac caggtagacc tcctcctgcc
     5341 gctcgtggcg gtgcacgcgc aggcgctggt gcggctgcag gatgagctgg ttgattccga
     5401 agctggtgac gccgagctca cgacgcagca ggtggaagta acccgcgtcg gggtcgatcg
     5461 aggtgaagga gaggctgggc tcagtcatcg ggcggcgaca ttctccccga cggccccgtc
     5521 ttctgcgtcg ccgagctcgt ggcggaccag cggcgcgacc tcggtcccga acagctcgat
     5581 cgagcgcatc acgtcggcgt gggggagggt gccgacgctg aactggatca ggaagcgctg
     5641 atggccgaag atcgcgtgct ggtggacgat cttcgccgcc acctcctcgg ggttgccgac
     5701 gaagttggcg ccgcgcaggg tgcggctcgc ctcgaaggtc tggcgggcca tcggcggcca
     5761 gccgcgctcg cggccgatcc ggtccatcat cagcgcgaag ggcgggtagg cggtgtcggc
     5821 ggcgacctgg gagtcggtgg caacgaagcc gtgcgagttg atgctgagcg ccggcggctg
     5881 gtggccggct tcgcgcgcgg cctcgcggtg catctccgcg aacggggcga agcgggccgg
     5941 ttc
//
LOCUS       DASRIG010000192         6602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_93448, whole genome shotgun sequence.
ACCESSION   DASRIG010000192 DASRIG010000000
VERSION     DASRIG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93448"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFB52_05285"
     CDS             complement(<1..784)
                     /locus_tag="VFB52_05285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO05779.1"
                     /translation="MPSAAAIETLAASYEQAPIATELVGIDGDFLAVNAALCELTGYE
                     RARLLGSASAGISHPDQALRESGLRDALLAGEIPRFKIEKRLVHASGQRLEVGAHVSL
                     VRGEDGAPICFLGQMLDITEHKRLERDLQHLADHDPLTGLLSRRRFEAELDRHVDHIA
                     RYGSEGAVLVLDIDHFKQVNDALGHGRGDELIIDVAGILQAQLRRSDRTARLGGDEFA
                     ILLPEANREQAAKVAAKLVEAVRREAVVLPGRSGRRVTVSVGV"
     gene            complement(834..1376)
                     /locus_tag="VFB52_05290"
     CDS             complement(834..1376)
                     /locus_tag="VFB52_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05780.1"
                     /translation="MKCLLPFALAAAVLVAGCGSDSGSSSAANVTAVAATGAAADRDA
                     MKHEDAMKGDQAMKSSGTKLKVVDSEYGSVVANGRGEALYLFDREQRGKSECYGACAK
                     AWPPVLTKGKPRAAGGADASLLGTTKRRNGKLQVTYAGQPLYYYVGDSPGTILCHDVD
                     EFGGTWLVVQPDGNPAGQSG"
     gene            1504..1935
                     /locus_tag="VFB52_05295"
     CDS             1504..1935
                     /locus_tag="VFB52_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZO05781.1"
                     /translation="MAIGLETAFGTSERPAARWAPPVRCVSGAIFVLFGIGKFADHGH
                     EVESFESYGLPSPDAFVYLIGLLEVGGGLLLLAGLATRLVALALAGNMVGAIVVSGIG
                     EGEVVPSLTLAPALLVAMLFLVWAGAGTRSLDTRLRSSWSR"
     gene            1923..3683
                     /locus_tag="VFB52_05300"
     CDS             1923..3683
                     /locus_tag="VFB52_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HZO05782.1"
                     /translation="MVALIGFAFLAGAATALSPCVLPVLPVALSAGVTGGRRRPLGVV
                     TGLTLSFAFATVALVYLIDALGLPGDLLRNLAIAVLLGFGITLLVPALSARVEVWISR
                     LMPAPQAERRGDGFGSGVLLGLSLGFVYAPCAGPILAGVITVSASQSFTAGRLGVALA
                     YALGSAVVLYFLMLGGRRLVSRLAPAAGKLQIATGAVMVIVALAMAGDYDARFQTAIA
                     NDLPDFVVNPTGELEENGAVREQLAGLRDGHGGIGARAAAAESDGASASKGEAAKLPV
                     LGVAPEFTDNERWFNTPGDRPLTLKELRGRVVLIDFWTYTCINCIRTLPYIKAWDERY
                     RDRGLTIVGVHTPEFPFEREAGNVEDAIEQNGLRYPVAQDNEYGTWNAYGNQYWPAKY
                     LIDAQGRVRYVHFGEGEYDETETAIRDLLAEAGEQPGKRARARIETASPRVTTPESYL
                     GSLRADRFVNGLIRPGTQRFQLPAGNLPDEGLAYGGRWRISPDAATAVAGSKLELNFG
                     ARRVFLVLGSAGGAPRPVRVLLDGRPIPSRYAGTDVHGGTATISSQRLYRLVELPQAE
                     RHLLTLEFAPGVSGYAFTFG"
     gene            3680..4411
                     /locus_tag="VFB52_05305"
     CDS             3680..4411
                     /locus_tag="VFB52_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05783.1"
                     /translation="MRLATETIADMATQGGRRTVLVVDDEPTISEVVSRYLERAGYEA
                     RTAADGLEAIRLVEETGPDLVVLDVMLPQVDGIRVLRHLREAEGGGVPVILLTAKGEQ
                     DDKLAGLRSGADDYVVKPFSPRELVARVDAVLRRAHPPEETTEPLRFDQLEIDARARR
                     VASDGEELALSQREFELLHFLASHPGQAFSRDELMDRVWETATPGDTSTVTVHVRRLR
                     TKIEPDPEQPRFIQTVWGVGYRFQP"
     gene            4408..5286
                     /locus_tag="VFB52_05310"
     CDS             4408..5286
                     /locus_tag="VFB52_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO05784.1"
                     /translation="MSGSLKRQAVLTAVAVALAIVALVLAARLMFLTERDALVAAILV
                     LGAGLVALRIATLAARERARAAAEATRRQLVAAVSHDLRTPIASLQLLVEAIDDEIVD
                     EPTRRRYIATMGTHVRSLSAMIDDLFELSRIEAGDIDWSMRQVELAVLVDETVQAMAP
                     EAGAKGVVIHSEIGAEPLAAQADPERIQRVLFNLIRNAIRHTPADGSVTIKAETGRDE
                     VEIEVADTGDGIAEEERERVFDAFFRGGEDAARGSDGAGLGLAVSRAIVETHGGRIWL
                     EPAASGTRVRFSLPRR"
     gene            5400..5732
                     /locus_tag="VFB52_05315"
     CDS             5400..5732
                     /locus_tag="VFB52_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO05785.1"
                     /translation="MPTDQLSATFAALADPTRRSLLSRLAEGEATVGELAEPLPISLP
                     AVSRHLKVLEGAGLVVRGRQAQWRPTRMQAEPLDEAADWIASRKRTWEARMDRLDAHL
                     ARNGDGDD"
     gene            5719..6189
                     /locus_tag="VFB52_05320"
     CDS             5719..6189
                     /locus_tag="VFB52_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZO05786.1"
                     /translation="MEMTDLEPPPGDDRTVLITRVFEAPREQVFRAWTDPDEVAGWYG
                     PAHMEAPRERITIDLRVGGRWELTMVAREGGGEFSIGYEIVELVEPELLVLKSDPMPE
                     MGMEEGTIVRIELHDHGEKTRLTLSDGPLPPPAREGAEAGWGSAMGKLGEHLAT"
     gene            complement(6423..6496)
                     /locus_tag="VFB52_05325"
     tRNA            complement(6423..6496)
                     /locus_tag="VFB52_05325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6460..6462),aa:Lys,seq:ttt)
BASE COUNT          936 a         2375 c         2299 g          992 t
ORIGIN      
        1 tcacgccgac gctcaccgtc acccggcgcc cgctgcgccc cggcagcacc accgcctccc
       61 ggcgcaccgc ctcgaccagc ttcgcggcga ccttcgccgc ctgctcgcgg ttggcctcgg
      121 gcagcaggat cgcgaactcg tcaccgccga gccgcgcggt tcgatccgag cgccgcagct
      181 gcgcctgcag gatgccggcg acgtcgatga tcagctcgtc gccgcggccg tggccgagcg
      241 cgtcgttgac ctgcttgaag tggtcgatgt cgaggaccag cacggcgccc tcgctgccgt
      301 agcgggcgat gtggtcgacg tggcggtcga gttcggcctc gaagcggcgg cggctcagca
      361 gcccggtcag cgggtcgtgg tcggcgaggt gctggaggtc gcgctccagg cgcttgtgct
      421 cggtgatgtc gagcatctgg ccgaggaagc agatcggcgc cccgtcctcg ccgcggacca
      481 gcgagacgtg ggcgccgact tcgagccgct gcccgcttgc gtggacgagc cgcttctcga
      541 tcttgaagcg cgggatctca ccggcgagta gggcgtcacg cagacccgac tcgcgcagcg
      601 cttggtcggg gtgggagatt ccagcgctgg cgctgccgag aaggcgggcg cgctcgtaac
      661 cggtcagctc gcagagcgca gcgttgaccg cgaggaagtc accgtcgatt ccgaccagct
      721 cggtcgcaat cggcgcctgc tcgtagctgg cggcgagggt ctcgatggca gccgcggatg
      781 gcatctccag ctggatcgga cgaccaaacg ggaatcctta accgcggttg cggctacccg
      841 gattgccctg ccgggttccc gtccggttgg acgacgagcc aggtgccgcc gaactcgtcg
      901 acgtcgtggc agaggatcgt gccgggtgag tcgccgacgt agtaatagag cggctgcccg
      961 gcataggtga cctggagctt gccgttgcgg cgcttggtgg taccgagcaa cgaggcgtcg
     1021 gcgccgccgg cggcgcgcgg cttgcccttg gtgaggaccg gcggccaggc cttggcgcag
     1081 gcgccgtagc actcgctctt gccgcgctgc tcgcggtcga agaggtagag cgcctcgccc
     1141 cggccgttgg ccacgacgga gccgtactcg gaatcgacca ccttcagctt ggtccccgag
     1201 ctcttcatcg cttggtcgcc cttcatcgcg tcctcgtgct tcatcgcatc gcggtcggcc
     1261 gcggcgccgg tggccgcaac ggcagtcacg ttggcggcgc tgctggaacc ggaatcgctt
     1321 ccgcagccgg cgaccaggac ggcggcggca agggcaaacg gcagtaggca cttcatggct
     1381 ctcctccttg gtctgggtct acgcccgagt attcgcgcga ccccttacga gtccattacg
     1441 ggactgacca gccagcgccg cagttcacac ttcggcaaga agtccttcct aggatcggcg
     1501 ccgatggcga tcgggttgga gacggcattc ggcaccagcg aacgcccggc cgcgcgctgg
     1561 gcgccgccgg tgcgctgcgt cagcggcgcg atcttcgtcc tcttcgggat cggcaagttc
     1621 gccgaccacg ggcacgaggt cgagtccttc gagtcctacg gcctgccctc ccccgacgcc
     1681 ttcgtctacc tgatcggcct gctcgaggtc ggcggcggcc tcttgctgtt ggccgggctg
     1741 gcaacgcggc tcgtcgccct cgccctggcc ggcaacatgg tcggcgcgat cgtcgtctcc
     1801 gggatcggcg agggcgaggt cgtgcccagc ctgacccttg ccccggcgct gttggtcgcg
     1861 atgctgttcc tcgtctgggc cggcgccggc acccgctcgc tcgacaccag gctgcgctcc
     1921 tcatggtcgc gctgatcggc ttcgccttcc tcgccggtgc cgccacggcg ctctctccct
     1981 gtgtgctgcc ggtgctgccg gtggcgctct cggccggggt gaccggggga cgtcggcggc
     2041 cgctcggcgt ggtcaccggc ctcaccctct ccttcgcctt cgcgacggtt gcgctggtct
     2101 acctgatcga cgcactcgga ctgcccggcg atctcctgcg caacctcgcg atcgcggtct
     2161 tgctcgggtt cgggatcacc ctcctggtgc cggcgctctc ggcccgggtc gaggtctgga
     2221 tctcacggct gatgccagca ccgcaggccg agcgccgcgg cgacggcttc ggctccggcg
     2281 tcctgctcgg cctcagcctc ggtttcgtct acgcgccctg cgccgggccg atcctcgccg
     2341 gggtgatcac ggtctccgcc tcgcagtcgt tcaccgccgg gcggctcgga gtcgccctcg
     2401 cctacgcgct cggcagcgcc gtcgtcctct acttcctgat gcttggcggg cgccgcctgg
     2461 tctcgcgcct ggccccggcg gcgggcaagc tgcagatcgc cactggcgcc gtgatggtga
     2521 tcgtcgcgct ggcgatggcg ggcgactacg acgcgcgctt tcagacagcg atcgccaacg
     2581 acctgcccga cttcgtcgtc aacccgaccg gcgagctgga ggaaaacggc gcggtccgcg
     2641 agcagctcgc cgggctgcgc gacggccacg gcgggatcgg cgcccgcgcc gccgccgcgg
     2701 aatccgacgg cgccagcgcc tccaagggcg aggcggcgaa gctgccggtg ctcggggtgg
     2761 cgccggagtt caccgacaac gagcgctggt tcaacacccc cggcgaccgc cccctgaccc
     2821 tgaaggagct gcgcggccgc gtcgtcctga tcgacttctg gacctacacc tgcatcaact
     2881 gcatccgcac cctcccctac atcaaggcct gggacgagcg ctaccgcgac cgcggcctga
     2941 cgatcgtcgg cgtgcacacg cccgagttcc cgttcgagcg cgaggccggc aacgtcgaag
     3001 acgcaatcga gcagaacggc ctccgttatc cggtcgccca ggacaacgaa tacgggacct
     3061 ggaacgccta cggcaaccag tactggccgg ccaagtacct gatcgacgcc cagggccggg
     3121 tccgctacgt ccactttggc gagggcgaat acgacgagac cgagaccgcg atccgcgacc
     3181 tgcttgccga agccggagag cagccgggga agcgggcccg cgccaggatc gagaccgcct
     3241 ccccgcgcgt cacgaccccc gagagctacc tcgggtcgct tcgcgccgac cgcttcgtca
     3301 acggcctgat ccgccccggc acccagcgct tccagctccc ggccggaaat ctgccggacg
     3361 agggccttgc ctacggcgga cgctggcgga tcagccctga tgccgccacc gcggtcgccg
     3421 gctccaagct cgagctgaac ttcggggcgc ggcgggtctt cctcgtgctc ggctccgccg
     3481 ggggagcgcc gcgccccgtc cgtgtcctgc tcgatggacg cccgatcccc agccgctacg
     3541 ccgggaccga cgtccacggc ggcaccgcga cgatctcctc ccagcgtctc taccggctgg
     3601 tcgaactgcc ccaggcagag cgccacctgc tgaccctcga attcgccccg ggcgtctccg
     3661 gctacgcctt caccttcgga tgagactcgc gactgagaca atcgccgaca tggcaaccca
     3721 gggcggtcgc cgcaccgtgc tcgtggtcga cgacgagccg acgatctccg aggtcgtctc
     3781 ccggtacctg gagcgggccg gctacgaggc gcgcaccgcc gccgacggat tggaggcgat
     3841 ccgcctggtt gaggagaccg gccccgacct cgtcgtcctc gacgtgatgc tgccccaggt
     3901 cgacggcatc cgggtgctcc gccacctgcg tgaggccgag ggtggcgggg tgccggtgat
     3961 cctgctgacc gcgaagggcg agcaggacga caagctggcc gggctgcgat ccggcgccga
     4021 cgactacgtg gtcaagccgt tctcgccgcg cgagctggtc gcccgggtcg acgcggtcct
     4081 gcgccgcgcc cacccgcccg aggagaccac cgaaccactg cgcttcgacc agctcgagat
     4141 cgacgcccgc gctcggcgcg tcgccagcga cggcgaggag ctggcgctga gccagcgcga
     4201 gttcgagctg ctgcacttcc tcgcctccca ccccgggcag gccttctccc gcgacgagct
     4261 gatggaccgc gtctgggaaa ccgcgacgcc gggcgacacc agcaccgtca ccgtccacgt
     4321 ccgccgcctg cggacgaaga tcgagcccga ccccgagcag ccgcgcttca tccagacggt
     4381 ctggggcgtt ggctacaggt tccagccgtg agcggctcgc tgaagcgcca ggcggtcctg
     4441 accgcggtcg ccgtggcgct ggcgatcgtc gccctcgtcc tcgccgcgcg gctgatgttc
     4501 ctcaccgagc gcgacgcgct ggtcgccgcg atcctcgtcc tcggcgccgg cctggtggcg
     4561 ctgcggatcg cgacgctggc cgcccgcgag cgcgcccgcg cggccgccga ggcgacccgc
     4621 cgccagctcg tcgccgcggt ctcccacgat ctgcgcaccc cgatcgcctc gctgcagctc
     4681 ttggtcgagg cgatcgatga cgagatcgtc gacgagccca cccgtcgccg ttacatcgcc
     4741 acgatgggca cccacgtgcg ctcgctgagc gcgatgatcg acgacctctt cgagctctcg
     4801 cggatcgagg ccggcgacat cgactggtcg atgcgccagg tcgagctcgc ggtcctggtc
     4861 gacgagaccg tgcaggcgat ggcgccggag gcgggcgcca aaggggtcgt gatccactcc
     4921 gagatcggcg ccgagccgct cgccgcccag gccgatcctg agcggatcca gcgcgtcctc
     4981 ttcaacttga tccgcaacgc gatccgccac accccggccg acggcagcgt cacgatcaag
     5041 gccgagacgg ggcgggacga ggtcgaaatc gaggtcgccg acacgggcga cgggatcgcc
     5101 gaggaggagc gcgagcgggt cttcgacgcc ttcttccgcg gcggcgagga cgctgcgcgc
     5161 gggtccgacg gggccgggct gggcctcgcc gtctcgcgcg cgatcgtcga gacccacggc
     5221 ggccgaatct ggctcgagcc cgccgccagc ggcacccgag tccgcttcag ccttccgcgc
     5281 agatagctcg catacgcact acataagggg agagagatga ccgactactc ggatcgcagt
     5341 gcgctgttcg tcaacaaggg cacggcctgt atttaacgat gtggttaaat acaggccaga
     5401 tgccgacgga ccagctcagc gccaccttcg ccgcacttgc cgacccgacc cggcgctcgc
     5461 tgctgagccg gctggccgag ggcgaggcga cggtgggcga gctggccgag ccgctgccga
     5521 tcagcctgcc cgccgtctcc cgccacctca aggtgctgga gggcgcgggc ctggtcgtgc
     5581 gcgggaggca ggcgcagtgg cggccgactc ggatgcaggc ggagccgctt gacgaggccg
     5641 ccgactggat cgcctcgcgc aagcggacct gggaagcccg aatggaccga ctcgacgcgc
     5701 acctggcgcg gaatggagat ggagatgact gacctcgagc cgccccccgg cgacgaccgc
     5761 accgtcctga tcacgcgcgt cttcgaggcg ccccgcgagc aggtgtttcg cgcctggacc
     5821 gatcccgacg aggtggccgg ctggtacggc cccgcccaca tggaagcacc gcgcgagcgg
     5881 atcacgatcg acctccgggt cggcgggcgc tgggagctga cgatggtggc ccgcgagggc
     5941 ggcggcgagt tctcgatcgg ctacgagatc gtcgagctgg tcgagcccga gctgctggtg
     6001 ctgaagtccg atccgatgcc ggagatgggc atggaggagg gcacgatcgt gcggatcgag
     6061 ctgcacgacc atggcgagaa gaccaggctg accctcagcg acggccccct ccctcccccc
     6121 gcccgcgagg gcgccgaagc cggctggggc tcggcgatgg ggaagctggg cgaacacctc
     6181 gcgacctaga ctttcgacgt ggcgtactcc gaggagcttg ccgagcggat ccgcgaaatt
     6241 atcgacgatc ggccgggcgt ggccgagcga aagatgttcg gtgggatcgc ctggatggtg
     6301 cttcatcacc gtcgaggccg caggcatcga ggcggataca gagctggggc gctgggtcga
     6361 cgccggagcg gacttcgccg agtcattgcc ggcaaagtga ccgccagcta gcggcttgag
     6421 aatgcccccg gcgggactcg aacccgcgac gcgcggctta aaaggccacc gctctaaccg
     6481 gctgagctac aggggcgcgc ggggtcaatg gtagacggcg cccgaccact cgtgtcggcg
     6541 gcctggaacc ggtgtcatga gaggtcgggg atgccctcgt aggcggtcga ggcctcggcg
     6601 tg
//
LOCUS       DASRIG010000193         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3759579, whole genome shotgun sequence.
ACCESSION   DASRIG010000193 DASRIG010000000
VERSION     DASRIG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3759579"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFB52_05330"
     CDS             complement(<1..314)
                     /locus_tag="VFB52_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZO05787.1"
                     /translation="MARILLGVSGGIAAYKALEFARLATLAGHGVRVVMTESAGRFVG
                     AASFEGIVGAPVLVSEFERDPMRGGFPGEEPPGHDPIGHLELAANCDALLIAPASANT
                     LA"
     gene            complement(357..590)
                     /gene="rpoZ"
                     /locus_tag="VFB52_05335"
     CDS             complement(357..590)
                     /gene="rpoZ"
                     /locus_tag="VFB52_05335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZO05788.1"
                     /translation="MIKPRVDKLLEHTDSHYAAVVVSAKRARQINSYFHNLGEGGFGE
                     YPPPMVETNAERNYLSMALEELAEGKLKYEYRT"
     gene            complement(583..1164)
                     /gene="gmk"
                     /locus_tag="VFB52_05340"
     CDS             complement(583..1164)
                     /gene="gmk"
                     /locus_tag="VFB52_05340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZO05789.1"
                     /translation="MSKVFVITGPSGVGKGTLIAKLLERVPDLELSVSATTRQPREGE
                     VNGRDYHFLTPEQFDQRVEEGDFLEFATYSGNLYGTLRTEVEKRLREGHSVVLEIEVQ
                     GARQVRAAMRESIQVFIAPPAPAVLRQRLDARGTDAPHAIDARLEVAAQEMAAQDEFA
                     YRVVNDDLGSAAAELERIVRAELGLSVDSPADD"
     gene            complement(1220..1558)
                     /gene="mihF"
                     /locus_tag="VFB52_05345"
     CDS             complement(1220..1558)
                     /gene="mihF"
                     /locus_tag="VFB52_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HZO05790.1"
                     /translation="MQAPPVKTSSLAAPERSLDQRMEALKRANDIRTARAKLKKDLKA
                     GRAGIHGILLDPPEYVMTAKVFDMLLAVPKYGRVKTNRILNQCRISPSKTIGGLSERQ
                     RAELVGLLRR"
     gene            complement(1668..>2296)
                     /locus_tag="VFB52_05350"
     CDS             complement(1668..>2296)
                     /locus_tag="VFB52_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HZO05791.1"
                     /translation="EDLPQLAELPVPLIVSVMATGRPEFSRLVEAVGGREEVAAIELN
                     VSCPNVHSGLIVGEQPSETEALLQTLRPLTPKPLIVKLTPNVADPAAVAVAAEEGGAD
                     AVSLINTLKGTAIDPKSGEPALAAGHGGLSGPAVRPVALHQLRTVRAAVAIPAIGMGG
                     ISTGADAAEFIAAGASLVAVGTENFRDPRAGARVAAELESARPLGAAA"
BASE COUNT          363 a          773 c          778 g          382 t
ORIGIN      
        1 ttggcgagcg tgttggcgct cgccggtgcg atcagcagcg cgtcgcagtt ggcggccagc
       61 tcgaggtggc cgatcgggtc gtgacccggg ggctcctccc ccgggaagcc accgcgcatc
      121 gggtcgcgct cgaactcgga gacgaggacc ggggcaccga cgatgccctc aaacgaggcg
      181 gcgccgacga agcggcccgc cgactcggtc atcacaaccc gcacaccgtg gccagccagc
      241 gtcgccaggc gcgcgaactc gagcgccttg taggcggcta tcccaccgct gacgccaagc
      301 aggattcggg ccatctcggt cggctccctc ggaaccgcgc cgctagtcca gcagcgctag
      361 gtgcggtact cgtatttgag cttgccctcg gcgagctctt cgagagccat cgagagatag
      421 ttcctctccg cgttggtctc gaccatcggc ggcgggtact caccgaagcc gccctctccg
      481 aggttgtgga agtagctgtt gatctgacgt gcgcgcttgg ccgatacgac gaccgcggcg
      541 tagtgagaat cagtgtgctc cagaagcttg tctactcgtg gcttaatcat cggccggcga
      601 gtctacagac agcccgagct cggcgcgcac gatccgctcc agctcagcgg ccgcgctccc
      661 gagatcgtcg ttgacgacgc ggtaggcgaa ctcgtcctgg gcggccatct cctgcgccgc
      721 cacctcgagc cgggcgtcga tcgcgtgggg tgcgtcggtg ccgcgggcgt cgagccgctg
      781 gcgcagcacc gccggggccg gcggggcgat gaagacctgg atcgactcgc gcatcgcggc
      841 gcgtacctgg cgggccccct ggacctcgat ctcgagcacc accgagtggc cctcgcgcag
      901 gcgcttctcg acctcggtgc gcagcgtccc gtagaggttg ccgctgtagg tggcgaactc
      961 gaggaagtcg ccttcctcga cccgctggtc gaactgctcg ggtgtgagga agtgatagtc
     1021 gcgcccgttt acctctccct cgcgcggctg ccgcgtggtc gccgagaccg agagctcgag
     1081 atcgggcaca cgttcgagca gcttggcgat caaggtgccc ttgccgaccc ccgagggtcc
     1141 ggtgattacg aagactttcg acatggttca gcgccgcccc gcggccgcgc aactcaaaca
     1201 ggtggaccgg acagcgaggc tatcgacgca ggagcccgac gagctccgca cgctgtcttt
     1261 cggacaggcc accgatggtc ttcgacggcg aaatccggca ctgattgagt attcgattgg
     1321 tcttcacccg tccgtacttc ggtacggcta gaagcatgtc gaagaccttg gcggtcatca
     1381 catactcggg cggatcgagc aggattccgt ggatcccggc gcggccggcc ttcaggtcct
     1441 tcttcagttt ggcgcgcgcg gtgcgaatgt cgttcgctcg cttcagcgcc tccatcctct
     1501 ggtcgaggga cctttcggga gccgcgagcg aggacgtttt gactggaggg gcctgcatgt
     1561 gggcggcgag tatagccgcg ggtgagggcc atgcaagcga agtacggcga tttgcccaca
     1621 aattcatcta gacctctagt cgaggtctag cactgaggag gcgacgacta ggcggctgcg
     1681 cccaatggac gtgcggattc gagctccgcg gcaacccgcg cgccggcccg cggatcgcgg
     1741 aaattctcgg tcccaaccgc aacgaggctt gcgccggcgg cgatgaactc ggccgcgtcg
     1801 gcgccggtcg agatcccgcc catgccgatc gccgggatcg cgacggcggc gcgaaccgtg
     1861 cgtagttgat gcagagcgac cggccgcacc gccgggcccg agaggccacc gtggccggca
     1921 gccaatgccg gctcgccgct tttcggatcg atcgcggtgc ccttcagggt gttgatcagc
     1981 gagaccgcgt cggcgccccc ctcctcggcg gcaaccgcga ccgccgccgg gtcggcgacg
     2041 ttgggggtca gcttcacgat cagcggcttc ggggtcagcg ggcgcagcgt ctgcagcaaa
     2101 gcctcggtct cgctgggctg ctcaccgacg atcagaccgg agtggacgtt gggacaggag
     2161 acgttcagct cgatcgccgc aacctcctct ctccccccca ccgcttcgac caatcgagag
     2221 aactcgggac ggccggtcgc catcaccgaa acgatcagcg gcaccggcag ctcggcgagc
     2281 tgcggcaggt cctcgg
//
LOCUS       DASRIG010000194         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2169065, whole genome shotgun sequence.
ACCESSION   DASRIG010000194 DASRIG010000000
VERSION     DASRIG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169065"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFB52_05355"
     CDS             <1..418
                     /locus_tag="VFB52_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05792.1"
                     /translation="ALLAIVAAPFAVLALLALVDLVSGANAHLTRSVLDAGGLGDLSD
                     VAQRRLQLSANSFGRPVLLVFLPVVAILAGLAILRRDRLREWVRDVPAVRAGLLGALA
                     ATAVGTLANDSGALLLEVGTAYLLVFTGFAWAESRD"
     gene            497..2770
                     /locus_tag="VFB52_05360"
     CDS             497..2770
                     /locus_tag="VFB52_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO05793.1"
                     /translation="MNRHVEALAEEFLGRGHDVRVLAPVDPPGRVSRVLHRAAAEQRE
                     LPEYLVPMGRSAAFGANGAVSNLAPFPSGVVKPRRHVRKEGFDVIHVHEPLVPLVGWN
                     ATLGARTPVVGTFHTYSTKPLPNYIANAVGARRMFNRLSARIAVSEAAAWTGRRWYGG
                     EYTIIPNGVDVDAAPSVPKPAGEELRILFVGRPEERKGLPILLTAFGALVEHVPSRLT
                     VIGAEAEDVQRYLTDPELMDSIDVRGRVSGERLWAELHEADVLCAPSLSGESFGMVLT
                     EAFAAGTPVIASAIAGYSDVVTDGVDGLLVPPADPQRLAEELQRVYHEPERLRAMGEA
                     ARRSAQRYAWPRIADEVTEVYERAVAVPRPETSGEKVDHWLGIRPADGLPKRPAEKLP
                     SLDPAPAQAGKGKRKVARRLGLGIAGLLGVGLTVLAAQKIGVDKVVESIVRSNLTLVL
                     VACALMATSLFFRAASWYWIARAALPNRPIRRRDVTSATMIGVLMSATLPARLGEPAR
                     ALALARRTGRMRETFPVLLGTLVSQTMLNLVALAMLGVIIVSTTPLFHSGTQKIFAFS
                     MVPLLLLVVVIIAPTLMRRNGNGRLARIGATLHRALIQVRAGLAVFREPRRGAMAAAA
                     QLGAWAIQLAACWALLYALNLDGKAGIGAAAAVLFAVNVTAVVPATPSNIGVFQLAVI
                     SVLHTGFGISTPDALAYGVILQAVEIATAVALGLPALVREGLTWSDLRVQALSTAPVH
                     LEPKPAPRDSRRGGVRI"
     gene            2893..>3293
                     /locus_tag="VFB52_05365"
     CDS             2893..>3293
                     /locus_tag="VFB52_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZO05794.1"
                     /translation="MPDAVQEEEVRAGSRALALFANPLNARVLRAHMEGPRRLSDVQE
                     VIGWSAESTIRAAISNLCEVGALEKQRVGDSARAVATDLSEVGKELLFVADELETWLA
                     RNPAGPIAPDSEGAKRAVKALAGGWNSTLVH"
BASE COUNT          447 a         1213 c         1099 g          534 t
ORIGIN      
        1 cgccctgctc gcgatcgtcg ccgccccgtt cgcggtcctg gcgctgctgg cgctcgtcga
       61 tctcgtctcc ggggccaacg cccacctgac ccgctcggtc ctcgacgccg gcggtctcgg
      121 tgacctctcc gacgtcgccc agcgccgcct gcagctctcc gcgaacagct tcggccgccc
      181 ggtgctgctg gtcttcctgc cggtcgtcgc gatcctcgcc gggctggcga tcttgcgccg
      241 cgatcggctt cgggagtggg ttcgcgacgt tccggcggtg cgcgcgggcc tgctcggggc
      301 gctcgccgcc accgcggtcg gcacgctcgc caacgactct ggtgccctgc tgctcgaggt
      361 tggcaccgcc tatctgctcg ttttcaccgg atttgcctgg gccgagagcc gggactaggc
      421 ccggccaatg ccctaccctt agagacctgt gcgcatcggt ctcgtctcgc catattcctg
      481 gtcttaccag gggggcgtga accggcacgt cgaagccctc gccgaggagt tcctcggtcg
      541 cgggcacgat gtccgcgtcc tcgctcccgt cgaccctcct gggagggtca gccgtgtcct
      601 gcaccgcgcc gcggcggaac agcgcgagct gcccgagtac ctggtcccga tggggcggag
      661 cgccgccttc ggtgccaacg gtgcggtctc gaacctcgcg ccgttccctt cgggagtcgt
      721 caaaccccgc cggcacgtgc gcaaggaagg cttcgacgtg atccacgtgc atgagccgct
      781 ggtgccgctg gtcggctgga acgcgacgct cggcgcgcgc acgcctgtcg tcggcacctt
      841 ccacacttac tcgaccaagc cgctccccaa ctacatcgcc aacgcggtcg gcgcgcggcg
      901 gatgttcaac cggctctcgg ctcggatcgc cgtctccgag gcggccgcct ggaccggacg
      961 ccgctggtac ggcggcgagt acacgatcat ccccaacggg gtcgacgtcg acgccgcgcc
     1021 gagcgtgccg aagccggccg gagaggagct gcgaatcctc ttcgtcgggc gccccgagga
     1081 gcgcaagggc ctgccgatcc tgctgaccgc gttcggcgcg ctggtcgagc acgttccctc
     1141 ccggctgacc gtgatcggtg ccgaggcgga ggatgttcag cgctacctca ccgaccccga
     1201 gctgatggac tcgatcgacg tgcgcggccg ggtctccggc gagcggctct gggccgagct
     1261 gcacgaggcc gacgtgctct gcgcgccctc gctctcaggc gagagcttcg gcatggtcct
     1321 gaccgaggcc ttcgccgccg gcacgccggt gattgcttcc gcgatcgccg gctacagcga
     1381 cgtcgtcact gacggggtcg acggcctgct ggttccgccc gccgatccgc agcgcctggc
     1441 ggaggagctg cagcgcgtct accacgagcc cgagcggctg cgggcgatgg gggaggccgc
     1501 gcggcgcagc gcccagcgct acgcctggcc gcggatcgcc gacgaggtga ccgaggtcta
     1561 cgagcgcgcg gttgcggtcc cgcgaccgga gacgagcggc gagaaggttg atcactggct
     1621 gggtatccgg cctgccgacg gtttgccgaa gcggccggcg gagaagctgc cctcgctcga
     1681 cccggcgccc gcccaggccg gcaaaggcaa acgcaaggtg gcgcggcgcc tcggtctcgg
     1741 catcgccggg ttgctcggcg tcggcctcac ggttctcgcc gcgcagaaaa tcggcgtcga
     1801 caaggtcgtc gaaagcatcg tccgctctaa cctgaccctg gtcctcgtcg cctgcgctct
     1861 gatggcgacc tcactcttct tccgcgccgc ctcctggtac tggatcgccc gcgcggcgct
     1921 gccgaatcgc ccgatccgcc gccgcgacgt cacctcggcg acgatgatcg gcgtgctgat
     1981 gtcggcgacc ttgccggccc gcctcggcga gccggcgcgc gctcttgcgc tggcgcgccg
     2041 caccggccgc atgcgcgaaa ccttcccggt tctgttggga acgctggtct cgcagacgat
     2101 gctgaacctg gtcgcgctgg cgatgctcgg cgtgatcatc gtctcgacca cgccgctctt
     2161 ccactccggc acccagaaga tcttcgcctt cagcatggtt ccgctgctcc tgctcgtggt
     2221 cgtgatcatc gcgccgaccc tgatgcgccg caacggcaac ggccggctcg cccggatcgg
     2281 cgcgactctg caccgggctc tgatccaggt gcgcgcgggt ctcgccgtct tccgtgagcc
     2341 gcgccgcggc gcgatggcgg cggccgccca gctcggcgcc tgggcgatcc agctggccgc
     2401 ctgctgggcg ctgctctacg cgctgaacct cgacggcaaa gccgggatcg gggccgccgc
     2461 cgcggtgctc tttgcggtca acgtgaccgc ggtagtgccg gcgaccccgt ccaacatcgg
     2521 cgtcttccag ctcgccgtga tcagtgtgct ccacaccggt ttcggcatca gcacgccaga
     2581 cgcactcgcg tacggcgtaa tcctgcaggc agtcgagatc gcgaccgcgg tcgccctagg
     2641 cctgcccgcc ctggttcgcg agggactcac ctggagcgat ctccgggtcc aggccctctc
     2701 gaccgcaccg gtccacctgg agccgaagcc ggcgccacgc gactcccggc gtggcggcgt
     2761 gcggatctaa aagccggaac cacctggctc ttaccatcag ttaaagactg cttagtaata
     2821 aggaacacac gagtgttcta ggattgcttc ggtaacgcca aagtctcgac cagtgccaca
     2881 gggagcactg cagtgcccga cgccgtccaa gaagaagagg tccgcgcggg aagccgcgcg
     2941 ctcgcactct tcgccaaccc gcttaacgct cgcgtcctcc gtgctcacat ggaggggccg
     3001 cgccgcctgt ccgacgtgca ggaggtgatc ggctggtcgg cggagtcgac gatccgcgcg
     3061 gcgatctcga acctctgcga agttggcgcc cttgagaagc aacgcgtcgg cgactccgcc
     3121 cgggccgtgg cgaccgacct cagcgaggtc ggcaaggagc tcctcttcgt cgccgacgag
     3181 ctggagacct ggctggcccg aaaccccgcc ggcccaatcg cacccgacag cgagggtgcc
     3241 aagcgcgccg tcaaagccct cgcaggcggc tggaactcaa ccctggtcca cga
//
LOCUS       DASRIG010000195        11001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2965962, whole genome shotgun sequence.
ACCESSION   DASRIG010000195 DASRIG010000000
VERSION     DASRIG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965962"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..612
                     /locus_tag="VFB52_05370"
     CDS             88..612
                     /locus_tag="VFB52_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO05795.1"
                     /translation="MIDGVMSLPREGERIVGGGLDATVKVTTEYPSLLSSFEIVVPPG
                     YDVGAHLHEIGEENFFVVEGELEMFCFDPVDRSIPDWHEWRSADGRGPMRGGPGTFMF
                     VPEGTPHAFGNPTDEPARMFFQSSVPGGHENYFHELADLLNATGGKPDPAASEELRKR
                     WHIEQLTPIGVART"
     gene            complement(711..1184)
                     /locus_tag="VFB52_05375"
     CDS             complement(711..1184)
                     /locus_tag="VFB52_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO05796.1"
                     /translation="MISVSYKIPVNPEGTDVTLTRADVWKGLEAKAHNALPYVPSMTY
                     CEVQDEGENWIQREIEFRGERFGERITLEPQNTVTFERTAGSVMGTILNEILETEDGE
                     LELRFSFNLELEGVEDGSREETDYEATMKGDYEKAVEATLAAIRRWVRDGEPASA"
     gene            complement(1181..1690)
                     /locus_tag="VFB52_05380"
     CDS             complement(1181..1690)
                     /locus_tag="VFB52_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO05797.1"
                     /translation="MSAAGDSLEARVQRIEDRIAISECVIRYAMSLDAADWDLFKDTI
                     ADPIHIDFSDWSGMEPRSWGREEWAGFARDVLSGFDARQHISPNHVITFQTEDEATCT
                     SYMFAQHLLRGAPGGDTLIMRGSYTNVLQRKPGGWEIKSMTQHFSWGEGNEEIFETSE
                     ERFKEGSAA"
     gene            complement(1687..2190)
                     /locus_tag="VFB52_05385"
     CDS             complement(1687..2190)
                     /locus_tag="VFB52_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO05798.1"
                     /translation="MSDSLEQRVQRIEDREAISECVIRYAASLDKADWELMKETIAEP
                     IFVDFTSWAELEARDYSREEWAGFARDVLSGFDARQHISPNHVITFESDDKATCTSYM
                     FAQHLIRDVPEGEVFIMRGSYTNVLQRNADGGWEIRSLTMHFTWGEGNENLHQLSQER
                     FKEGSAA"
     gene            complement(2209..2535)
                     /locus_tag="VFB52_05390"
     CDS             complement(2209..2535)
                     /locus_tag="VFB52_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05799.1"
                     /translation="MSEPQYHVAHLAQPSAVLASELERVFAASWTEVAHLRLAPGERL
                     QRPLTDSEAMVFVLGGAGTVRLGELTEPLREGISLTLFQGEPLDLEAAADEGLDLFLA
                     EMTVDS"
     gene            complement(2532..2936)
                     /locus_tag="VFB52_05395"
     CDS             complement(2532..2936)
                     /locus_tag="VFB52_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO05800.1"
                     /translation="MAIISDAHEGLAKLVGVHGGAGVMGWKRLGTGNMMASAVDSFEI
                     AELPPGSRAGKHVHSRTEELFYVLSGRALIGLGDEQVEVQAGDAILTGFRGIQSVDAI
                     GEEPYRMLVIEALPPEIVAGLPEHRPSEEVEA"
     gene            complement(3047..3472)
                     /locus_tag="VFB52_05400"
     CDS             complement(3047..3472)
                     /locus_tag="VFB52_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZO05801.1"
                     /translation="MTFFVDANVFVYLVAESPASESCLRVLEAVASGEADGRTSPAVL
                     QEVWHVTERLLGKRQDGLIESALTIFSPLVPVTAEALSKALAVEGPSLDAQDRLHVGT
                     CAAEGIETILSGDRGFDEVAGIRRVDPFDAAAVEQLLAA"
     gene            complement(3469..3753)
                     /locus_tag="VFB52_05405"
     CDS             complement(3469..3753)
                     /locus_tag="VFB52_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05802.1"
                     /translation="MSMFSERLQILVSKDQRRRLEREAKRRNASVASVIRDAVDAELG
                     GGSRNEKLAAVEAIRELPRVPFLPVEELNRIYDESYTESVLRGIPGLSPE"
     gene            complement(3887..4666)
                     /gene="fabI"
                     /locus_tag="VFB52_05410"
     CDS             complement(3887..4666)
                     /gene="fabI"
                     /locus_tag="VFB52_05410"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZO05803.1"
                     /translation="MILEGKRVLVTGVVNRRSIAFAIAERAQAEGAEVLLTSFGRMMR
                     MTERAANRLDPVPDVLELDVNDDDHLAALRSELESRWGRVDGAVHAIAFAPADALGGD
                     FLATPRASAATAFETSAFSYKALADAVAPLMSDGGGLVGLDFDATVAWPSYDWMGVAK
                     AALESVNRYLARDLGPHGIRANLVAAGPIGTAAASGISGFSDLVKVWDEGAPLGWEVE
                     DALPVADAALFLLSDLSRAITGEIVHVDGGVHAIGAATPAA"
     gene            complement(4663..5157)
                     /locus_tag="VFB52_05415"
     CDS             complement(4663..5157)
                     /locus_tag="VFB52_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO05804.1"
                     /translation="MDSTLSRDFDALTVGDRFVSRGRTITEADLVGFSTLTGDFHPLH
                     TDAEWAAESEFGGRIAHGMLLLSYCVGLVPLDPEHVLALRGFESVAFKRPVRIGDTIH
                     VEGKLDSKKELDEASGLAVFAWKIVNQRGESVALVKVRVLWRRAPQVPDAAPATEQRE
                     EVYL"
     gene            complement(5157..5843)
                     /locus_tag="VFB52_05420"
     CDS             complement(5157..5843)
                     /locus_tag="VFB52_05420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZO05805.1"
                     /translation="MPVAEPPTDPDLSTAHPVGRGKVVSSYVALGDSFTAGRESIEAL
                     RWADRLANWLRAVNPELTYANLAVDGATSAEVLVQVPQGLELNPDLITVICGANDILL
                     TSRPDVVGYEERFSTILERLREGAPEAAILTTTAPETWHFLELRPRTKARLVKALADL
                     NAVTRSVAARHHVPCLNVAGHPGLTDPANFSADGLHPSSQGHERAVEEIALSLQAHFG
                     IQGDFQGGHV"
     gene            complement(5843..7267)
                     /locus_tag="VFB52_05425"
     CDS             complement(5843..7267)
                     /locus_tag="VFB52_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZO05806.1"
                     /translation="MATATAKPAVVPGLPSDAPLPLLERLCDPGTFRPLRSGAVSIRL
                     GDRATPGDGVLGGAGEVNGRPVFCYSQDPKYLGGSLGEVHADTIVRLLRMAGDAGAPV
                     VGFVRSGGARLQEGHAGLAGYGRIFRASVELSRKVPQISILSGVSAGGGAYSPALTDF
                     VFMTEDARMFLTGPKVVQEAMGEEISMEALGGPKVHGRNGVAHLVRPDADGAIEAARH
                     LLGLLPQSIARPPALVEAIEADGSDPGSHVPAEQRKVYDVRDVATAILDQGSFLELSP
                     KWAPNIVTGIARLEGRSVGLIANQPRALGGVIDAAASEKAALFVESCNRFGLPLLVFV
                     DTPGFMPGKAQEEAGVIRHGASLLRAFAGASVPKVTLVLRKAFGGAAITMNSKELGAD
                     VVYAWPGAQVGVMAARQAVGIVHHRALKEEGGPSRDELAEAYEAEHLTAPAAAASGWV
                     DEVIEPAHSRDRLAWALRSMGGSF"
     gene            complement(7275..8471)
                     /locus_tag="VFB52_05430"
     CDS             complement(7275..8471)
                     /locus_tag="VFB52_05430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZO05807.1"
                     /translation="MTRQVVITGVGAITPLGIGAETLIERWIAGECGIEDGEGRCRDF
                     VATDFLSKKEARRSDRFAQMAVVAAREATAQAGIAEELPYEAHRIGCVVGTGIGGLPS
                     LEENQRKLIERGEKAVSPLAVPLMMGNAAAAAVAMQYGLHGHCYGVVSACAAGAHALG
                     DASRIIRAGDADMVVSGGTEAAISGVATAAFGAMEATSPTGISRPFDARRDGFVIGEG
                     AGILVLEEKAAAEARGATILGELLGYGATCDAFHLTAPQPEGTEAARAISLAIADAGL
                     TAPDVDYVNAHGTSTPLNDRSETMALKAALGERAGDIPVSSTKSAIGHLLGAAGAVEA
                     IATVEALRRRTAPPTVGWEEPEEGLDLDYVPGQSKPIENGDGPLVAISDSFGFGGHNA
                     VLCLAA"
     gene            complement(8468..8716)
                     /locus_tag="VFB52_05435"
     CDS             complement(8468..8716)
                     /locus_tag="VFB52_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZO05808.1"
                     /translation="MPTDVTTENVEKVIYDGLVELGTERDELSREATLEDLDIDSLDL
                     VELAQIVEDEFGVELKGDDVKDVKTVGEVIDLVVSKSS"
     gene            complement(8747..9475)
                     /gene="fabG"
                     /locus_tag="VFB52_05440"
     CDS             complement(8747..9475)
                     /gene="fabG"
                     /locus_tag="VFB52_05440"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZO05809.1"
                     /translation="MARPEGCALVTGSSRGIGAATAIALAADGWPVRVNYRSDEQGAR
                     EVAEKIEAAGGSATVVQGDVADPAAVKALLEPGEDGPVLCLVNNAGLRLDGLSPQIED
                     DQWQRVIDVNLTATFRATREALKPMLRARYGRIVNVSSVVGLHANAGQANYAASKAGV
                     IGFTKTVAVEVARRGVTVNAVAPGLIETALTEEIVGGELEKAIPARRVGTPEEAAACI
                     RFLASEEASYVTGTTLTVDGGLSA"
     gene            complement(9475..10473)
                     /locus_tag="VFB52_05445"
     CDS             complement(9475..10473)
                     /locus_tag="VFB52_05445"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HZO05810.1"
                     /translation="MPESAALAREQAAEPRTTVGAEIAGLGAAVPETVVTNAPIAERF
                     GVDERWIVKRTGIESRHYAAPGDLLFELAATAGQRALDDAGIAATELDLIVVATTSND
                     ELMPATASRVGAALGAGTAGAFDVNAACTGFVAGIDVVTGLIESGRARNALLIGADFM
                     SRLTDPDDRATVTVFADGAGAVVMRGTSGAGRIGPVVLGSDGDNADLIFIKRDKGVIE
                     MQGHETFRHAVDRLCSATAEALDAAGVGQDEIDLFVYHQANARILRAVGERIELPLER
                     VVDCIAELGNTSAATVPLALAEARREGRLEPGARVLLGAFGAGLNWGATVIEWGTD"
     gene            complement(10466..>11001)
                     /locus_tag="VFB52_05450"
     CDS             complement(10466..>11001)
                     /locus_tag="VFB52_05450"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZO05811.1"
                     /translation="MEEAAQEAPGGMLASLGGEEPTVREIAGRFGLTVANENAPGQLV
                     LSGPSEGLGEARKELRASGAKAIRLPVAGAFHSPLMSGAAANFRAALDQVDFQAPSLP
                     VYSSTAAAPFEDIPARLAAALTEPVIWCQTLGRMQEAGAGTFLESGPGDVLTGLVRRT
                     LPEAEARSLAETEAVGA"
BASE COUNT         1527 a         3819 c         3981 g         1674 t
ORIGIN      
        1 gctgatcacg tattacgtag tccattgact atttcgtaat caacgtgaga taatggctcc
       61 gggttttcaa ccacggagga ggtaggtgtg atcgatggag tgatgtccct gccgcgcgag
      121 ggcgagcgga tcgtcggcgg cggtctcgac gcgaccgtga aggtgacgac cgagtacccg
      181 tcgctcttgt ccagcttcga aatcgtcgtc ccgcccggct acgacgtcgg cgcccacctg
      241 cacgaaatcg gcgaggagaa cttcttcgtc gtcgagggcg aactggagat gttctgcttc
      301 gacccggtcg accgctcgat ccccgactgg cacgagtggc gctcggccga cgggcgcggg
      361 ccgatgcgcg gcggccccgg caccttcatg ttcgtgcccg agggcacccc gcacgcgttc
      421 ggcaacccaa ccgacgagcc cgcccggatg ttcttccagt cctcggtccc cggcggccac
      481 gagaactact tccacgaact agccgacctg ctcaacgcga ccggcggcaa accggacccg
      541 gccgccagcg aagagctgcg caagcgctgg cacatcgagc agctgacgcc gatcggcgtc
      601 gcccgcacct aggcgctcga aaacgctggg gcggcgctct gcggcgtccc tgaccgaacg
      661 ccgcagagcg ccgccccggg cgctcgcgga accttggtga ggcgggccgg ctaggcgctg
      721 gcgggctcgc cgtcgcggac ccagcggcgg atcgcggcga gggtcgcttc gaccgctttc
      781 tcgtagtcgc ccttcatcgt cgcttcgtag tcggtctcct cccgggagcc gtcctcgacg
      841 ccttcgagct cgaggttgaa ggagaagcgc agctccagct cgccgtcctc ggtctcgagg
      901 atctcgttga ggatcgtgcc catcacgctg ccggcggtgc gctcgaaggt gacggtgttc
      961 tgcggctcca gcgtgatccg ctcgccgaag cgctcgccgc ggaactcgat ttcgcgctgg
     1021 atccagttct cgccctcgtc ctggacctcg cagtaggtca tcgagggtac gtacggcagg
     1081 gcgttgtggg ccttcgcctc cagccctttc cagacgtcgg cgcgggtcag ggtgacgtcg
     1141 gtgccctcgg ggttgacggg gatcttgtag ctgacggaga tcatgcggcg cttccttcct
     1201 tgaatcgttc ctctgaggtc tcaaaaatct cttcgttgcc ttcgccccag ctgaagtgct
     1261 gggtcatgct cttgatctcc cagccgcccg gcttgcgctg cagcacgttg gtgtagctgc
     1321 cgcgcatgat cagcgtgtcg ccgccggggg cgccacgcag caggtgctgg gcgaacatgt
     1381 aggacgtgca ggtcgcctcg tcctcggtct ggaaagtgat cacgtggttg gggctgatgt
     1441 gctggcgcgc gtcgaagccg ctgagcacgt cgcgggcgaa ccccgcccac tcctcgcgtc
     1501 cccaggagcg cggctccatc cccgaccagt cgctgaagtc gatgtggatc gggtcggcga
     1561 tcgtgtcctt gaacaggtcc cagtcggcgg cgtcgagcga catcgcgtag cggatcacgc
     1621 actcgctgat cgcgatgcgg tcctcgatcc gctggacccg cgcctcgagc gaatcgccgg
     1681 ccgcgctcat gccgcgctgc cctctttgaa ccgttcctgg gagagctggt ggaggttctc
     1741 gttgccctcg ccccaggtga agtgcatggt caggctgcgg atctcccagc cgccgtccgc
     1801 gttgcgctgc agcacgttgg tgtagctgcc gcgcatgatg aagacctcgc cctcggggac
     1861 gtcccggatc aggtgctggg cgaacatgta agaggtgcag gtcgccttgt cgtcggactc
     1921 gaaagtgatc acgtggttgg ggctgatgtg ctggcgggcg tcgaagccgc tgagcacgtc
     1981 gcgggcgaac ccggcccatt cctcgcggct gtagtcgcgt gcctcgagct ccgcccagct
     2041 ggtgaagtcg acgaagatcg gctcggcgat cgtctctttc atcagctccc agtcggcctt
     2101 gtcgagcgag gccgcatagc ggatcacgca ctcgctgatc gcttcgcggt cttcgatgcg
     2161 ctgtacgcgc tgctccagtg aatcgctcac gttcctctcc tccttggact acgaatcgac
     2221 ggtcatttca gccaggaaca ggtccaggcc ctcgtcggcg gccgcctcga ggtcgagcgg
     2281 ctcgccctgg aagagggtca gcgagatgcc ctcgcgcagc ggctcggtca gctcgccgag
     2341 ccgcacggtg ccggcgccgc cgagcacgaa gaccatcgcc tcggagtcgg tcagcggacg
     2401 ctgcaggcgc tcgccggggg cgaggcgcag gtgggcgacc tcggtccagg aggcggcgaa
     2461 gacgcgctcc agctcggaag cgaggacggc gctcggctgg gccaggtggg cgacgtggta
     2521 ctgcggctcg ctcacgcctc gacctcctcg ctcgggcggt gctcgggcag gccggcgacg
     2581 atctccggag gcagcgcctc gatcaccagc atccggtagg gctcctcgcc gatcgcgtcg
     2641 accgactgga tcccacggaa gccggtgagg atcgcgtcgc cggcctgcac ttccacctgc
     2701 tcgtcgccga gcccgatcag ggcgcggccc gagagcacgt agaagagctc ctcggtgcgc
     2761 gagtgcacgt gcttgccggc ccggctcccg ggcggcagct cggcgatctc gaaggagtcg
     2821 acggcgctgg ccatcatgtt gccggtcccg aggcgcttcc aacccatcac cccggcaccg
     2881 ccgtgcaccc cgacgagctt cgccagcccc tcgtgggcgt cgctgatgat cgccaccgca
     2941 gctcctccta gtcgcgtgaa atcgaactca ggataaaacc tataatgatt cgtggtgata
     3001 tgcaaccgca ccgcgaggcg gcatgcgcgg cgagcccggt ggggcttcag gccgcgagca
     3061 gctgctcgac cgcggcggcg tcgaacggat cgacgcggcg gatgccggca acctcgtcga
     3121 agccgcgatc gccgctgagg atcgtctcga tcccctccgc ggcgcaggtg ccgacgtgca
     3181 acctgtcctg ggcatccagc gacgggccct ccacagccag cgccttggac aaggcctccg
     3241 cagtgaccgg caccagcggc gagaagatcg tcaacgcgct ttcgatcaag ccgtcctgtc
     3301 gcttcccgag cagccgttcg gtgacgtgcc agacctcctg gagcacggcg ggagaggtgc
     3361 gcccgtccgc ctcgccgctc gccaccgcct ccagaacccg caggcaactc tccgacgcgg
     3421 ggctctcggc gaccaagtac acgaagacgt tggcgtcgac gaagaaggtc attctggcga
     3481 cagtccgggg atcccccgca gaaccgactc ggtgtacgac tcgtcgtaga tgcggttcag
     3541 ctcctcgacc ggcaggaacg gaacccgcgg cagctcccgg atcgcttcga cggcggcgag
     3601 cttctcgttg cgggaaccgc cgcccagctc ggcgtcgacc gcgtcgcgga tgacgctggc
     3661 caccgaggcg ttgcgccgct tggcctcgcg ttccaagcgg cggcgctggt ccttgctgac
     3721 gaggatctgg agccgctccg aaaacatgct cataagatac acatcagtat ggacgggcgg
     3781 aggggctggc tgggcgagcg cgggcatggc cagcaccgtg acaggcgaaa gcgcacgcgt
     3841 tctgcgcacg tcgaatcaat tccggaactt ctcgtgcgcg ggagcgctac gcggcggggg
     3901 tagcggcgcc gatcgcgtgc acgccgccgt cgacgtggac gatctcgccg gtgatcgcac
     3961 gcgagaggtc ggagagcagg aagagggcgg cgtcggcgac cgggagcgcg tcttcgacct
     4021 cccagccgag cggcgctccc tcgtcccaga ctttgacgag gtcggagaag ccggagatgc
     4081 cggaggcggc ggcggtgccg atcggtccgg cggcgacgag gttggcgcgg atgccgtgcg
     4141 ggccgaggtc gcgggcgagg tagcggttga ccgactccag cgccgccttc gcgaccccca
     4201 tccagtcgta ggagggccag gcgacggtgg cgtcgaagtc gagcccgacg aggccgccgc
     4261 cgtcgctcat cagcggggcg accgcatcgg cgagcgcctt gtaggagaag gcgctggtct
     4321 cgaaggcggt cgcggcgctc gcccgcgggg tggcgaggaa gtcgccgccg agcgcgtcgg
     4381 cgggcgcgaa ggcgatcgcg tgcacggcgc cgtcgacccg cccccagcgg ctctcgagct
     4441 cactgcgcag cgccgccagg tgatcgtcgt cgttgacgtc gagctcgagc acgtcgggca
     4501 ccgggtccag acggttggcg gcgcgctcgg tcatccgcat catccgcccg aagctggtca
     4561 gcagcacctc ggccccctcg gcctgggcgc gctcggcgat ggcgaaggcg atgctgcggc
     4621 ggttgacgac tccggtgacg aggacccgct tgccctcgag gatcacaggt agacctcttc
     4681 gcgctgctcg gtcgccggcg ccgcgtcggg cacctgcggg gcgcggcgcc agagcacccg
     4741 cacctttacc agggcgaccg actcgccgcg ctggttgacg atcttccagg cgaagacagc
     4801 tagcccgctg gcctcgtcga gctccttctt cgagtcgagc ttgccctcga cgtggatcgt
     4861 gtcgccgatc cgcaccggtc gcttgaaggc gacgctctcg aagccgcgca gggccagcac
     4921 gtgctcgggg tcgagcggga ccaggccgac gcagtaggag agcagcagca tcccgtgcgc
     4981 gatccggccg ccgaactcgc tctccgccgc ccactcggcg tcggtgtgca gcgggtggaa
     5041 gtcgccggtc agggtcgaga agccgaccag gtcggcctcg gtgatcgtgc gcccgcggga
     5101 gacgaagcgg tcgccgacgg tgagcgcgtc gaagtcgcgg gaaagcgtcg agtccatcag
     5161 acgtgtcctc cttggaagtc gccctggatt ccgaagtggg cctgcagcga gagggcgatc
     5221 tcctctacgg ctcgctcgtg gccctgggag gacgggtgca gcccgtcggc ggagaagttg
     5281 gcggggtcag tcagccctgg atgaccggcg acgttgagac aaggcacgtg gtggcgggcg
     5341 gcgaccgagc gggtgaccgc gttgaggtcg gccagagcct tgaccagacg cgccttggtc
     5401 cgcggccgca gttcgagaaa gtgccaggtc tccggcgcgg tcgtggtcag gatcgccgcc
     5461 tcgggggcgc cttcgcgcag ccgctcgagg atcgtcgaga agcgttcttc gtagccgacg
     5521 acgtcgggcc gcgaggtcag caggatgtcg ttggcgccgc agatcaccgt gatcagatcc
     5581 gggttcagct ccagcccctg cggcacctgc acgagcactt cggcgctggt cgcgccgtcg
     5641 accgcgaggt tggcgtaggt gagctccggg ttgaccgcgc gcagccagtt ggcgaggcgg
     5701 tcggcccagc gcagcgcctc gatcgattcg cgaccggcgg tgaagctgtc cccgagcgcg
     5761 acgtagctgc tgacgacctt cccccgcccc accggatggg ccgtgctcag gtcggggtcg
     5821 gtcggaggct cggcgaccgg catcagaagc tgccgcccat cgagcgcagc gcccaggcca
     5881 gccggtcgcg gctgtgggcg ggctcgatta cctcgtcgac ccagccgctg gcggcggccg
     5941 cgggcgcggt caggtgctcg gcctcgtagg cctcggcgag ctcgtcgcgc gagggcccgc
     6001 cctcctcttt cagcgcccgg tggtggacga tcccgaccgc ctggcgcgcc gccatcacgc
     6061 cgacctgcgc gcccggccag gcgtagacga cgtcggcgcc gagctctttc gagttcatcg
     6121 tgatcgccgc gccgccgaac gccttgcgca ggaccagcgt cactttcggc accgaggcgc
     6181 cggcgaaggc gcgcagcagc gaggcgccgt ggcggatcac gccggcctcc tcctgggcct
     6241 tgccgggcat gaacccgggg gtgtcgacga aaaccagcag cggcagcccg aagcggttgc
     6301 aggactcgac gaagagcgcc gccttctccg aggcggcggc gtcgatcacg ccgccgagcg
     6361 cccgcggctg gttcgcgatc agcccgaccg agcggccctc gaggcgggcg atcccggtga
     6421 cgatgttcgg cgcccacttc ggcgagagct cgaggaagct gccctggtcg aggatcgcgg
     6481 tggccacgtc gcgcacgtcg tagaccttgc gctgctcggc ggggacgtgc gagccggggt
     6541 cgctgccgtc ggcctcgatc gcctcgacca gcgccggcgg ccgcgcgatc gactgcggca
     6601 acaggccgag caggtggcgg gcggcctcga tcgcgccgtc ggcgtcgggc cggaccaggt
     6661 gggcgacgcc gttgcggccg tgcaccttgg gaccgccgag cgcctccatg ctgatctctt
     6721 cgcccatcgc ctcctggacc actttcggcc cggtgaggaa catccgcgcg tcctcggtca
     6781 taaacacgaa gtcggtcagc gccggggagt aggcgccgcc gcccgcggac acgccgctga
     6841 ggatcgagat ctgcgggacc ttgcgcgaga gctcgacgct ggcccggaaa atgcggccgt
     6901 agccggcgag gccggcgtgg ccctcctgca ggcgggcgcc gccggagcgg acgaagccga
     6961 ccaccggggc accggcgtcg ccggccatcc gcagcaggcg gacgatcgtg tcggcgtgga
     7021 cttcgccgag gctgccgccg aggtacttgg ggtcctgcga gtagcagaag accgggcggc
     7081 cgttgacctc gccggccccg ccgagcacgc cgtcgccggg ggtcgcccgg tcgccgagtc
     7141 ggatcgagac cgcgccgctg cgcagcggcc ggaaggtgcc cgggtcgcag agccgctcga
     7201 gcagcggcag cggcgcgtcg gacggcaggc ccgggacgac ggcgggtttg gcggtagcgg
     7261 tggccatctc tacctcacgc cgccagacag aggacggcgt tgtgaccgcc gaagccgaag
     7321 gagtcggaga tcgcgaccag ggggccgtct ccgttctcga tcggtttcga ctgtccgggc
     7381 acgtagtcga ggtcgagccc ctcttcgggc tcttcccagc cgaccgtcgg cggggcggtg
     7441 cggcggcgca gcgcctccac cgtggcgatc gcctcgaccg ccccggcggc gccgagcagg
     7501 tggccgatcg ccgacttggt cgagctcacc gggatgtcgc cggcgcgctc gccgagcgcc
     7561 gccttcagcg ccatcgtctc gctgcggtcg ttgagcgggg tcgaggtgcc gtgcgcgttg
     7621 acgtagtcga cgtcgggcgc ggtcaggccc gcgtcggcga tcgccaggct gatcgcccgc
     7681 gccgcctcgg tcccttccgg ctgcggcgcg gtcaggtgga aggcgtcgca ggtagcgccg
     7741 tagccgagca gctcgccgag gatcgtcgcc ccgcgcgctt ccgccgcagc tttctcctcg
     7801 agcacgagga tgccggcgcc ctcgccgatc acgaagccgt cgcggcgagc gtcgaagggg
     7861 cgggagatgc cggtcggcga ggtcgcttcc atcgccccga aggcggcggt cgcgacgccc
     7921 gagatcgccg cctcggtgcc gccgctgacg accatgtcgg cgtcgccggc gcggatgatc
     7981 cgcgaggcgt cgccgagcgc gtgggcgccg gccgcgcagg cggagacgac gccgtagcag
     8041 tgaccgtgca ggccgtactg catcgccacc gcggcggccg cggcgttgcc catcatcagc
     8101 ggcacggcca gcggcgagac cgctttctcg ccgcgctcga tcagcttgcg ctggttttcc
     8161 tccagcgagg gcaggccgcc gatcccggtc ccgaccacgc agccgatccg gtgcgcctcg
     8221 tagggcagct cctcggcgat cccggcctgc gcggtcgcct cgcgggcggc gacgaccgcc
     8281 atctgggcaa agcggtccga gcgccgcgcc tccttcttcg agaggaagtc cgtggcgacg
     8341 aagtcgcggc agcggccctc gccgtcctcg atcccgcact caccggcgat ccagcgctcg
     8401 atcagggtct ccgcgccgat ccccagcggg gtgatcgcgc cgactcccgt gatgactacc
     8461 tggcgagtca cgaggacttg ctcaccacca ggtcgatgac ctcgccgacg gtcttcacgt
     8521 ccttgacgtc gtcgcctttg agctcgacgc cgaactcgtc ctcgacgatc tgcgcgagct
     8581 cgaccaggtc gagggagtcg atgtcgaggt cctcgagggt tgcctcgcgg gagagctcgt
     8641 cgcgctcggt gccgagctcg accaggccgt cgtagatcac cttctcgacg ttctccgtgg
     8701 tcacatcagt gggcattggt tctccttgtt ctcgttggga agttggttag gcgctgagcc
     8761 cgccgtcgac ggtcagcgtg gttccggtca cgtaggaggc ctcttcggag gccaggaaac
     8821 ggatgcaggc ggcggcctcc tccggggtgc cgacgcggcg cgcggggatc gccttctcca
     8881 gctcgccgcc gacgatctcc tcggtcagcg cggtctcgat caggcccggg gcgaccgcgt
     8941 tgacggtgac gccgcggcgc gcgacctcga ccgccacggt cttggtgaag ccgatcacgc
     9001 cagccttcga ggcggcgtag ttggcctggc cggcgttcgc gtgcaggccg acgacggagc
     9061 tgacgttgac gatccggccg tagcgggcgc gcagcatcgg cttcagcgct tcgcgggtgg
     9121 cgcggaaggt ggcggtgagg ttgacgtcga tcacccgctg ccactggtcg tcctcgatct
     9181 ggggcgagag cccgtcgagg cgcaggccgg cgttgttgac caggcagagc accgggccgt
     9241 cctcgccggg ctcgagcagc gccttcaccg cggccgggtc ggcgacgtcg ccctggacca
     9301 cggtggcgct gccgcccgcg gcctcgatct tctcggcgac ctcgcgggcg ccctgctcgt
     9361 cggagcggta gttgacccgc accggccagc cgtcggcggc gagcgcgatc gcggtcgcgg
     9421 cgccgatccc ccgcgaggag ccggtaacca gcgcgcatcc ctccgggcgg gccatcagtc
     9481 ggtcccccac tcgatcacgg tcgcgcccca gttgaggccg gcgccgaagg caccgagcag
     9541 gacccgggcg ccgggctcca ggcgtccctc gcggcgcgcc tccgcgagcg ccagcggcac
     9601 cgtcgccgcc gaggtgttgc cgagctcggc gatgcagtcg acgacgcgct ccagcggcag
     9661 ctcgatccgt tcgccgaccg cgcgcaggat gcgggcgttg gcctggtgat agacgaagag
     9721 gtcgatctcg tcctgcccga cgccggccgc gtcgagcgct tcggcggtgg ccgagcagag
     9781 ccggtcgacc gcgtggcgga aggtctcgtg gccctgcatc tcgatcacgc ctttgtcgcg
     9841 cttgatgaag atcaggtcgg cgttgtcgcc gtcggagccg agcacgaccg ggccgatccg
     9901 gccggcgccg ctggtcccgc gcattaccac cgcgccagcg ccgtcggcga agacggtgac
     9961 cgtggcgcgg tcgtcggggt cggtcaggcg cgacatgaag tcggcgccga tcagcagcgc
    10021 gttgcgggcg cggccggact cgatcagccc ggtgacgacg tcgatcccgg cgacgaaacc
    10081 ggtgcaggcg gcgttgacgt cgaaggcgcc ggcggtgcca gcgccgagcg cggcgccgac
    10141 ccgcgaagcg gtcgccggca tcagctcgtc gttggaggtg gtggcgacga cgatcaggtc
    10201 gagctcggtc gcggcgatcc cggcgtcgtc gagcgcccgc tgcccggccg tggcggccag
    10261 ttcgaacagc aggtcgccgg gcgccgcgta gtggcgcgac tcgatcccgg tccgtttgac
    10321 gatccagcgc tcgtcgacgc caaagcgctc ggcgatcggg gcgttggtga cgacggtctc
    10381 cgggacagcg gcgccgaggc cggcgatctc ggcgccgacg gttgttcgcg gctcggcggc
    10441 ctgctcgcgg gcgagggcgg cggactcagg cacccacggc ctccgtctcg gccagcgagc
    10501 gcgcctcggc ctcgggcagg gtgcggcgga cgaggccggt gagcacgtcg ccggggccgg
    10561 actcgaggaa ggtgccggcg ccggcctcct gcatgcggcc gagggtctgg caccagatca
    10621 ccggctcggt cagggcggcg gcgaggcggg ccggaatgtc ttcgaagggt gcggccgcgg
    10681 tcgaggagta gacgggaagt gacggcgcct ggaaatcgac ctggtcgagc gcggcgcgga
    10741 agttggcggc ggcgccgctc atcagcggcg agtggaaggc gcccgcgacc ggcagccgga
    10801 tcgccttggc gccgctcgcg cgcagctctt tacgggcctc ccccagcccc tcgctcggcc
    10861 cagagagcac gagctggccg ggcgcgtttt cgttggcgac ggtgagccca aagcggccgg
    10921 cgatctcgcg cacggtcggc tcctcgccgc cgagcgaggc gagcatcccg cccggcgcct
    10981 cctgcgcggc ttcctccatc a
//
LOCUS       DASRIG010000196         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3761036, whole genome shotgun sequence.
ACCESSION   DASRIG010000196 DASRIG010000000
VERSION     DASRIG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761036"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1311)
                     /gene="cax"
                     /locus_tag="VFB52_05455"
     CDS             complement(175..1311)
                     /gene="cax"
                     /locus_tag="VFB52_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZO05812.1"
                     /translation="MSAQGQRRDGPAGFLLSAQGWPYLLTPFIVAAVALELAHASATL
                     VFACAALGIIPTAALMGLATEELAARSGPGIGGFLNVTFGNAAELIIAVFLLAEGLQE
                     VVKASIVGSILGNLLLVLGASMVVGGIGRARQTFNRTAANAQATMLFLAVAALIMPAI
                     YALVAGGGLPAVDSELVDFGSDLEELSVGVAVILGISYLAGLLFSLRTHRDVFNPPYA
                     EQEHEGFGWSVRRSILMLAIAGAAVALVSEVLVGSISEASESIGLSEFFVGIIVVAVV
                     GNAAEHWVAVLVARKNKMDLAVNIAVGSAAQIALFVAPILVLLSFVIGPEPMALVFNG
                     FELGAVLVAVLIAAYVTQEGESNWFEGVQLLAVYVVFGLAFYFA"
     gene            complement(1388..2533)
                     /locus_tag="VFB52_05460"
     CDS             complement(1388..2533)
                     /locus_tag="VFB52_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05813.1"
                     /translation="MTRIRTYFALAALALFAPVLVAGCGGDDSSDVDPQTVIDETFNN
                     DETVSSGDLSLTLSGSAEGDQGASFEANLSGPFQGDPDDPAAIPQLDWTGSLSAEGAG
                     QSFDFEGGITVTEDNAYVEYGGSAYEVGAETFGRFKKLAERASAQQAETEGLSFTDAF
                     QQGCEQSLQAQGGDTSACDIDFQSWLGDLSTEGDEEIEGTETTHISGSLNVDTMLQDL
                     IGLGAALPEASSAGVPSEEQVQQIADAISEASFDLYSGVDDRILRGVDFNLAVDPSAI
                     PDAEAAGVESVDASFSLRLASVNEEQEISAPSDAQPLEELLGQFGVDPSALGGLGGLG
                     ALGGAGGGLPGGGGGGGGAAGGNADAYLDCIAEAQTPDEINACASQL"
     gene            2905..>3259
                     /locus_tag="VFB52_05465"
     CDS             2905..>3259
                     /locus_tag="VFB52_05465"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZO05814.1"
                     /translation="MLFADLSGYTAVSERLDPERMKTLIDRALRRLGEEVERHGGTID
                     KYIGDNVMGVFGAPIAHEDDPERAVRAGLAMQAAMEEVNERIAADVGANFALRVGINS
                     GEVLAGRIGDGYTVIG"
BASE COUNT          536 a         1157 c         1122 g          444 t
ORIGIN      
        1 tcagcgtctc cggcgtcgcg gtgccgactg cagcgaggat gctgccgacc gcgccttcgc
       61 cgatctgcag cttgtgcccg atccactcga ccgcgcccgt gaagagcagc gcaccggcca
      121 gcacgatcgc gaagctgacg acgaggagca ggtattccat ctaggcgccc accgtcatgc
      181 gaagtagaag gcgaggccga agacgacgta gacggcgagc agctggacgc cctcgaacca
      241 gttcgactcg ccctcctggg tcacgtaggc ggcgatcagc acggcgacga gcaccgcgcc
      301 cagctcgaac ccgttgaaca ccagggccat cggctcgggg ccgatcacga acgacagcag
      361 gaccaggatc ggggcgacga agagcgcgat ctgcgccgcc gacccgaccg cgatgttgac
      421 cgcgaggtcc atcttgttct tgcgggcgac gagcaccgca acccagtgct cggcggcgtt
      481 gccgacaacg gcgaccacga tgatcccgac gaagaactcg ctcagcccga tcgactccga
      541 cgcctcggag atcgagccga ccaggacctc ggagacgagc gcgaccgccg caccggcgat
      601 cgcgagcatc aggatcgagc gtcggacgct ccagccgaag ccctcgtgct cctgctccgc
      661 gtagggcggg ttgaagacgt cgcggtgggt gcgcagcgag aagagcaggc ccgcgagata
      721 ggaaatgccg aggattaccg cgaccccgac cgagagctcc tcgaggtcac tgccgaagtc
      781 gacgagctcg gagtcgacgg cggggagccc gccgcccgcg accagggcgt agatcgccgg
      841 catgatgagc gccgcgaccg cgagaaacag catcgtcgcc tgcgcgttgg ccgccgtgcg
      901 gttgaaggtc tgacgcgccc gcccgattcc gccgacgacc atcgaggccc cgagcaccag
      961 cagcaggttg ccgaggatcg agccgacgat cgacgccttg accacctcct ggaggccctc
     1021 ggcgagcagg aacacggcga tgatcagctc ggccgcgttg ccgaaggtga cgttgagaaa
     1081 cccgccgatc cccgggcccg agcgcgccgc cagctcctcc gtcgccaggc ccatcagcgc
     1141 cgcggtcggg atgatcccca gcgcggcgca ggcgaagacg agggtcgccg acgcatgcgc
     1201 cagctccagc gccaccgcgg cgacgatgaa cggggtgagc aggtagggcc agccctgggc
     1261 gctgagcagg aatcccgccg gcccatcccg cctctgtccc tgcgcgctca tccgggcagc
     1321 ctagtaacgc gctcgcgaaa ggggccccgg cgggcgccgg agcccttgct tcgctcgtcg
     1381 atcgagccta gagctggctc gcgcaggcgt tgatctcgtc gggcgtctgc gcctcggcga
     1441 tgcagtcgag atacgcgtcg gcgttgccgc cggccgcgcc gccaccgcca ccgccgcctc
     1501 ccggcaagcc gccaccggct ccgccgaggg cgccgagccc gccgaggccg ccgagcgcgc
     1561 tcgggtcgac cccgaactgc ccgagcagct cctcgagcgg ctgggcgtcg ctgggcgccg
     1621 agatctcctg ctcctcgttg acgctcgcca ggcgcagcga gaagctggcg tcgacgctct
     1681 ccacgccggc cgcctcggca tccggaatcg cgctcgggtc gaccgccagg ttgaagtcga
     1741 cgccgcgcag gatgcggtcg tcgacaccgg agtagagatc gaagctcgcc tccgagatcg
     1801 cgtcggcgat ttgctggacc tgctcctcgg acggcacgcc cgcggacgag gcctcgggga
     1861 gcgccgcgcc gagcccgatc aggtcctgga gcatcgtgtc gacgttcagg ctgccgctga
     1921 tgtgcgtggt ttcggtgccc tcgatctcct cgtcgccctc ggtcgagagg tccccgagcc
     1981 agctctggaa gtcgatgtcg caggccgagg tgtcaccgcc ctgcgcctgc aacgactgct
     2041 cgcagccctg ctggaatgcg tccgtgaacg agaggccctc ggtctcggcc tgctgcgcgg
     2101 aggcccgctc ggcgagcttc ttgaaccggc cgaaggtctc ggcgcccacc tcgtaggcgc
     2161 tgccgccgta ctcgacgtag gcattgtcct cggtgaccgt gatcccacct tcgaagtcga
     2221 agctctgccc ggcgccctcc gccgaaagcg agccggtcca gtcgagctgc gggatcgccg
     2281 ccgggtcgtc gggatcgccc tggaacggac cgctgaggtt tgcctcgaag ctggcaccct
     2341 gatccccctc ggccgacccg ctcagggtga gcgagagatc gccgctggag acggtttcgt
     2401 cgttgttgaa ggtctcgtcg atgaccgtct gcgggtcgac gtcgctcgag tcgtcgccgc
     2461 cgcaaccggc gaccaggacc ggtgcgaaca gcgcgagcgc cgcgagcgcg aagtaggtgc
     2521 gaatgcgggt caagttcttg gctccctcgt aatcgttgtg gaaggcggcc agactagcga
     2581 acccgaatac ggccggaacg ccgattcgtc acacatccac gccgcggagc gcctgacgca
     2641 agcgcccgag cggaacacca ccaccgcgcg gcgtatcctg accacgtgac ctgtccgagt
     2701 tgcggcacgg agaacccgga tcgagcacgg ttctgcatgt cgtgcggtgc cgggctgccc
     2761 gtcagctgcc cgtcgtgcgg agccgagaac ccggatcagg cgaagttctg catcgagtgc
     2821 ggaagtccgc tcgcagccgc ggaggcgcgg cccgcgcgcc cgcgggccga cgagcctccc
     2881 cccgaggaac ggcgcagggc gaccgtgctg ttcgccgacc tctccgggta caccgccgta
     2941 tccgagcgcc tcgacccgga gcgtatgaag acgctcatcg accgcgccct gcggcggctc
     3001 ggcgaggagg tcgagcgaca cggcggcacg atcgacaagt acatcggcga caacgtcatg
     3061 ggtgtcttcg gcgccccgat cgctcacgag gacgaccccg agcgagcggt gcgcgccggg
     3121 ctcgccatgc aggccgcgat ggaggaggtc aacgagcgga tcgccgccga cgtcggggcc
     3181 aacttcgctc tgcgagtggg gatcaactcc ggcgaggtcc tagccggcag gatcggcgac
     3241 ggctacacgg tgatcggcg
//
LOCUS       DASRIG010000197         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2171601, whole genome shotgun sequence.
ACCESSION   DASRIG010000197 DASRIG010000000
VERSION     DASRIG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171601"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..698)
                     /locus_tag="VFB52_05470"
     CDS             complement(36..698)
                     /locus_tag="VFB52_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO05815.1"
                     /translation="MNTSARKPSAMQRVVSDPVSRRRFLALTGGTTAAGALLAACGNH
                     DDEGDATAATTEDDMASQFGEGDLGIVNYALTLEYLEAAFYADVAKSGLFKGDDLALI
                     ERIGRAEQEHVDALTAAAKQLGGQPAAEPKAEFPLDDAKSVLELAATVENLGAAAYLG
                     QAAAISDPEILAAALSIHSVEGRHAAVLNTLVGMPATPDGAFAQGADMQTVLEEVQPF
                     IT"
     gene            complement(989..1915)
                     /locus_tag="VFB52_05475"
     CDS             complement(989..1915)
                     /locus_tag="VFB52_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HZO05816.1"
                     /translation="MPEGDLYEQIIEAVHANFGSHPGKRALHAKGSWAQGEFTATPAA
                     ARLSHAPHLQGETVTALVRFSNASGDPEAHDAARDGRGMAIKLRWDGGETDILTTTSP
                     TFVSRTPEEFLELMLLRRPDPATGQPDMAKLGAYFGDHPESVPAVQAVLMKEPIASFA
                     TAEYFSPHSFGLVDADGNRTWVRYHLRPEAGEQRLADDEAKALGRDYLHEEIAERLGA
                     GNVAFDLRLQLAGPDDPLDDPTAVWSQDGELVDAGRLELTAIADDPERDGHIDVFDPT
                     RVVAGIDLPDDPILHARPKAYSVSAYRRWDRD"
     gene            1959..2711
                     /locus_tag="VFB52_05480"
     CDS             1959..2711
                     /locus_tag="VFB52_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05817.1"
                     /translation="MDLGIDGRVALVLGASKGIGRGIAEGLAREGARVALASRSRERL
                     ETAVAEIDGETAAFEADTDDLERIAALPNEVAESLGPVEILVTNTGGPPTGEALDAGL
                     DAWRKAYESLVLAPRVLAEAVVPGMRERGWGRIVNVGSSSTEEPIPGLTLSNAHRPAL
                     IGFYKTLAREVAADGVTVNDIATGRFATDRLAANWGGREEMERGAPADVPAGRLGTPA
                     EYGDLVAFLASERAGYITGVSIPIDGGLLRSI"
     gene            complement(2708..>2956)
                     /locus_tag="VFB52_05485"
     CDS             complement(2708..>2956)
                     /locus_tag="VFB52_05485"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZO05818.1"
                     /translation="VTYVLWRLRFAFRPGLLFALYLVLAGAERFLIEFIRRNDDVAVG
                     LTEAQLISVAMILAGGVWLAVKANRGELRTGPAPAAGT"
BASE COUNT          400 a         1018 c         1091 g          447 t
ORIGIN      
        1 ctggcttctg catcactgct tcctttctcc ttgcgtcagg tgatgaacgg ctggacctct
       61 tcgagcacgg tctgcatgtc ggccccctgc gcgaacgcgc cgtccggtgt ggcgggcatg
      121 ccgacgaggg tgttgagcac cgccgcgtgg cgtccctcca ccgagtggat cgagagcgcc
      181 gcggcgagga tctccgggtc ggagatcgca gcggcctggc ccaggtacgc ggccgcgccg
      241 aggttctcca cggtcgccgc gagctcgagc accgacttgg cgtcatcgag cgggaactcg
      301 gccttcggct ccgccgccgg ctggccgccg agctgcttgg cggccgcggt gagcgcgtcg
      361 acgtgctcct gctcggcccg gccgatcctt tcgatcagcg ccaggtcatc gcccttgaac
      421 aatccgctct tggcgacgtc ggcgtagaag gccgcctcga ggtactcgag ggtgagcgcg
      481 tagttgacga tcccgaggtc gccctcgccg aactgcgagg ccatgtcgtc ttcggtggtc
      541 gccgcggtgg cgtcgccctc gtcgtcgtgg ttgccgcagg ccgcgagcag cgcgcccgcc
      601 gcggtcgtgc ctccggtcag cgcgaggaag cggcgccgcg agacggggtc ggagacgacg
      661 cgctgcatgg cgctcggctt gcgtgcggaa gtgttcatct gtggtctccc gtggtcgggt
      721 cccggggagt ggcctccccg cttgccctcc ctctacggga gctccggcgg cgcggatcac
      781 agggaccggg gggagctgca caaatggaca acggccgccg ggcgccgaag ccgtgtccat
      841 ctagccgcct atttcacggt caaatggaca cggccgcgct tgggcgccgg cagtgtccat
      901 ctatgcgtgc tcccgccgaa gcgcatcccc acttgacccg tcctcccgcg cgcaccccgg
      961 agccgatgcc caccgtgccg ccgaaccgct agtcgcggtc ccagcgccgg taggccgaga
     1021 ccgagtacgc cttcggccgg gcgtggagga tcggatcgtc cggaagatcg atcccggcga
     1081 cgaccctggt cgggtcgaag acgtcgatat gtccgtcacg ctcggggtcg tcggcgatcg
     1141 ccgtcagctc cagccgcccg gcgtcgacca gctcgccgtc ctgggaccag actgccgtcg
     1201 ggtcgtcgag ggggtcgtcg ggcccggcga gctggagccg aaggtcgaat gcgacgttgc
     1261 cggctccgag gcgctcggcg atctcctcgt ggagatagtc ccgccccagc gccttcgcct
     1321 cgtcgtcggc gaggcgctgc tcgcctgcct cgggtcgaag gtggtagcgc acccaggtgc
     1381 ggttcccgtc ggcgtcgacc agcccgaacg aatgcggcga gaagtactcg gcggtcgcga
     1441 agctcgcgat cggctccttc atcaggaccg cctgcaccgc cgggacggac tcgggatggt
     1501 cgccgaagta ggcgccgagc ttcgccatgt cgggctgtcc ggtcgccggg tcgggacggc
     1561 gcaacagcat cagctcgagg aactcctcgg gcgtgcggct gacgaaggtg ggcgacgtcg
     1621 tcgtgaggat gtcggtctcg ccgccgtccc agcgcagctt gatcgccatc ccgcgcccgt
     1681 cccgggcggc atcgtgggcc tccgggtcgc cgctcgcatt cgagaagcgg accagcgcgg
     1741 tgacggtctc gccctgtagg tgaggagcgt ggctgaggcg cgcggcggcg ggcgtcgcgg
     1801 tgaactcgcc ctgcgcccac gagccctttg cgtgcagcgc ccgcttgccg ggatgcgagc
     1861 cgaagtttgc gtgaaccgcc tcgatgatct gctcgtagag gtcgccctcc ggcatcgcct
     1921 cgccacccta tcccttcgac tgggtagcgt catgacccat ggacctcggg atcgacggcc
     1981 gggtggcgct ggtgctcggc gccagcaagg gcatcggccg cgggatcgcg gaggggctcg
     2041 cgcgcgaggg cgcgcgcgtg gccctcgcga gccgatcgcg ggaacggctc gaaacggcgg
     2101 tggcggagat cgacggcgag accgccgcct tcgaggcgga caccgacgac ctggagcgga
     2161 tcgcggcgct tccgaacgag gtcgccgaat cgctcgggcc ggtcgagatc ctcgtcacga
     2221 acaccggcgg accgccgacg ggcgaggcgc tcgacgccgg actcgacgcc tggcgaaagg
     2281 cgtatgagtc gctggtcctg gcgccgcggg tcctggccga ggcggtggtg cccgggatgc
     2341 gggagcgcgg ctgggggcgg atcgtcaacg tcggatcgag ctcgaccgag gagccgatcc
     2401 ccggcctgac cctctcgaat gcacaccggc ccgccctgat cgggttctac aagacgctgg
     2461 cgcgcgaggt ggctgccgac ggtgtgaccg tcaacgacat cgcgaccggt cgctttgcga
     2521 cggatcggct ggcggcgaac tggggtggcc gggaggagat ggaacgcggc gcgccggccg
     2581 acgtgccggc cggtcggctg ggcacgccgg cggagtacgg cgacctcgtc gccttcctcg
     2641 cctccgagcg cgccggctac atcaccgggg tctcgatccc gatcgacggc gggctgttgc
     2701 gctccatcta ggtcccggcg gccggggccg gccccgtgcg cagctcgccg cggttggcct
     2761 tcaccgcgag ccagacgccg ccggcgagga tcatcgcgac gctgatcagc tgggcctcgg
     2821 tgagcccgac ggcgacgtcg tcattgcggc ggatgaactc gatcaggaag cgctcggcgc
     2881 cggccagcac cagatagagg gcgaagagca gaccggggcg aaacgcgaag cgcagccgcc
     2941 agaggacgta ggtgac
//
LOCUS       DASRIG010000198         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_96869, whole genome shotgun sequence.
ACCESSION   DASRIG010000198 DASRIG010000000
VERSION     DASRIG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96869"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..899)
                     /locus_tag="VFB52_05490"
     CDS             complement(24..899)
                     /locus_tag="VFB52_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05819.1"
                     /translation="MFVFKAAVVGAGTMGGEIAQAIASSDIPVVLKDIDQEFVDHGLE
                     KAREVTKGQLDRLVEKEKLTEEQASARLEEVIGLIEGTTTYDSFGDVDLVIEAAPEKM
                     EVKQAVFAELDEVTPGHAILASNTSSLSISEMGEATLRPEKVVGFHFFYPASVMPLVE
                     IVEGDETDPEAVQAAYNFAQAIRKQPITCAEVPGFVVNRILNSAVGEIWRAQAEQGLS
                     IKKIDEAITAANVAPMGPFFLGDLLGLDTILHVAEHLQASYGDRFYVHPMMRELVEAG
                     KLGQKTGQGYYAHGG"
     gene            complement(975..2345)
                     /locus_tag="VFB52_05495"
     CDS             complement(975..2345)
                     /locus_tag="VFB52_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO05820.1"
                     /translation="MEAGGLRDRFDGELIGPDEAGYDEARSIFNSMIDRRPALIAQCE
                     SDADVAAALAHAREHGLEVAIRSGGHSVAGAGSVEGGLVIDMRRMNAVEIDPDARIAR
                     VGGGATWGDFDRAGQPHGLMTTGGRVSTTGVAGLTLGGGSTWFERKFGTACDHLESVT
                     LVTADGRRVTASESENPELFWALHGGGGNFGVATELVFRLSELPTVTLALLLWPADAG
                     PEVIRRYRDLFEAGAPEELGGAGAYITGPPEEFVPKQLQGQLVAGAVAFYAGGEAEAR
                     EALAPFLELAPEAEMIAEMPYSEAQSAIDDPPGYRNYWSAQYLDRFPDEAIDAFCRRA
                     HDMVVPSPSQHILFPLGGALARNAGEWPMPYRHAPWAVHPLGLWEDPGDDEQAKAWAR
                     GIVEDMRPYAIENVYLNFIPNEGEDRLIAGYGRENYERLAAVKAEYDPDNVFHLHHNI
                     KPLQPA"
     gene            complement(2434..>2768)
                     /locus_tag="VFB52_05500"
     CDS             complement(2434..>2768)
                     /locus_tag="VFB52_05500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO05821.1"
                     /translation="TLVWRSNPAGRGTVTERVLEHQPRRRHRIEFSDPESKGELLTRF
                     EIEGERTRVSLSYDYGLRRGGPFAWLTERLFVRGQVRASLQRSLLRFKYEAEEVATLG
                     DPTRRAPS"
BASE COUNT          405 a          975 c          937 g          451 t
ORIGIN      
        1 tctctgcagt cgcggtctcc acctcagcct ccgtgggcgt agtagccctg gccggtcttc
       61 tggccgagct ttccggcctc gaccagctcg cgcatcatcg ggtggacgta gaagcgatcg
      121 ccatagctcg cctgtaggtg ctcggcgacg tggaggatcg tgtcgaggcc gagcaggtcg
      181 cccaggaaga agggacccat cggcgcaacg ttggcggccg tgatggcctc gtcgatcttc
      241 ttgatcgaga ggccctgctc cgcctgggcg cgccagatct cgccgacggc gctgttgagg
      301 atccggttga cgacgaagcc cggtacctcg gcgcacgtga tcggctgctt gcggatcgcc
      361 tgcgcgaagt tgtaggcggc ctggacggcc tcggggtcgg tctcgtcgcc ctcgacgatc
      421 tcgaccagcg gcatcaccga ggcgggatag aagaagtgga agccgacgac cttctcgggc
      481 cgcagggtcg cctcgcccat ctccgagatc gaaagcgatg aggtgttcga ggcgaggatc
      541 gcgtggccgg gcgtgacctc gtcgagctca gcgaacaccg cctgcttgac ctccatcttc
      601 tccggggccg cctcgatgac gagatcgaca tcgccgaagc tgtcgtaggt ggtcgtcccc
      661 tcgatcaggc cgatcacctc ctcgagcctc gctgaggcct gctcctcggt cagcttctcc
      721 ttctcgacca gccggtcgag ctggcccttg gtgacctcgc gcgccttctc gaggccgtgg
      781 tcgacgaact cctggtcgat gtccttgagg acgactggga tgtcggacga cgcgatcgcc
      841 tgcgcgatct caccgcccat cgtgccggcg ccgacgacgg cggccttgaa gacgaacatt
      901 cggctgggac cctccggtgt cgctgaaaga gcgcgcggga ggctagccga tcgacgacgc
      961 ccggcgcgcg gcgttcaggc aggctggagc ggcttgatgt tgtggtgcag gtgaaagacg
     1021 ttgtcggggt cgtactcggc cttgactgcc gcgaggcgct catagttctc gcgcccgtag
     1081 ccggcgatca gccggtcctc gccctcgttg gggatgaagt tgagatagac gttctcgatc
     1141 gcgtacgggc gcatgtcctc gacgatgccg cgggcccacg ccttggcctg ctcgtcatca
     1201 cccgggtcct cccagagccc gagcgggtgc accgcccagg gcgcatgccg gtagggcatc
     1261 ggccactcgc ccgcgttgcg ggccagcgcg cccccgagcg ggaagaggat gtgctgcgat
     1321 ggcgacggca cgaccatgtc gtgcgcccgc cggcagaagg cgtcgatcgc ctcgtctgga
     1381 aagcggtcga ggtactgcgc ggaccagtag ttccggtagc cgggcggatc gtcgatcgcg
     1441 gactgggctt ccgagtaggg catctcggcg atcatctccg cctccggcgc gagctcgagg
     1501 aacggcgcca gcgcctccct cgcctcggcc tcaccacccg cgtagaacgc gacggccccg
     1561 gcgacgagct gaccctggag ctgcttgggg acgaactctt cgggcggccc cgttatgtag
     1621 gcgcccgcgc cgccgagctc ctccggggcg ccggcctcga acaggtcacg gtagcgacga
     1681 atcacttcgg ggccggcgtc cgccggccac agaagcaacg ccagggtgac cgtgggcaac
     1741 tccgatagcc ggaacacgag ctcggtcgcg acgccgaaat tgccgccgcc accgtgcagg
     1801 gcccagaaca gctccgggtt ctcggactcg ctcgccgtga cccggcggcc atccgcggtc
     1861 acgagcgtca ccgactccag gtggtcgcat gcggttccga acttgcgctc gaaccaggtc
     1921 gagccgccgc ccagcgtcaa cccggcaacc ccggtcgtcg agacccgccc gccggtcgtc
     1981 atcagcccgt gcggctgacc ggcgcggtcg aagtcgcccc acgtggcccc gccgccgacc
     2041 cgcgcgatac gcgcatcggg gtcgatctcg accgcgttca tccgccgcat gtcgatcacc
     2101 aggccgccct cgaccgaccc cgcgcctgcg acgctgtggc cgccactgcg aatggccacc
     2161 tccaggccgt gctcccgggc gtgcgctaag gcggcggcca cgtcggcatc cgactcgcac
     2221 tgggcgatca gcgccgggcg tcggtcgatc atcgagttga agatcgagcg tgcctcgtcg
     2281 taccctgcct cgtcgggccc gatcagctcg ccgtcgaatc gatcgcgcag tcctcctgcc
     2341 tccatgctcc agctcctctg tcggtggtgg tctgaccgta tccggtcggc cgccgaccgt
     2401 aaaggacatc cgcaggcggg tcagtgagcg tggtcacgaa ggcgcgcggc gggtcggatc
     2461 accgagcgtg gcgacctctt ccgcctcgta cttgaagcgc agcagggaac gctgaaggga
     2521 ggcgcgcacc tggccgcgga cgaacagccg ctccgtgagc caggcgaacg ggccgccccg
     2581 gcgcaggccg tagtcgtagc tcaggctgac ccgtgttcgc tcgccctcga tctcgaaccg
     2641 ggtcagcagc tcgcccttcg actccgggtc ggagaactcg atccgatgtc ggcggcgcgg
     2701 ctggtgctcg agtacccgct cggtcaccgt gccccgcccg gccgggttcg atcgccagac
     2761 cagcgtgc
//
LOCUS       DASRIG010000199         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3216906, whole genome shotgun sequence.
ACCESSION   DASRIG010000199 DASRIG010000000
VERSION     DASRIG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216906"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFB52_05505"
     CDS             complement(<1..802)
                     /locus_tag="VFB52_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZO05822.1"
                     /translation="MNWDSALADYDRDLRARGSAERTRRAYGVDLGGFVEWVGPQGLG
                     PTDVRHRDVRRYAAGLSTAGAAPATVARKLAAIRGLYGFLVRTERAGSNPAELVSSPK
                     RAEKLPSVLSTEQMRSLLERIPAGTPLELRDRAMLELAYSCGLRCEEIVNLDVGAVDF
                     ESEQVRVLGKGSKERILPIGEPAQRALERYLQKGRRALATEPREQALFLSKSGRRLSN
                     SDVTRRLGLWTREAALAAGVSPHSLRHSFATHLLEGGADLRTIQELLGH"
     gene            complement(843..1880)
                     /locus_tag="VFB52_05510"
     CDS             complement(843..1880)
                     /locus_tag="VFB52_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZO05823.1"
                     /translation="MGRKTKITLGVIVGLIVVLAAGAYVYDSTQKDKIAEGVTIAGVD
                     VGGMDADEAKAEVQRELLAPLKDTLKVELDDESWTLPGDKLKIRANIDEAVEEAVEES
                     QDGGLPTRLVRYVSGSELDKPVTAELDYSKSAINRFVRHVAEEVNRDPVDASVEPSSD
                     SLNVVSGKAGLKLRDNELTKDLEDAVLNASAPRTVVAHVHKTKPDVTTKEVAKEYPAY
                     LTLDRSTFTLRLWQDLKNTKTYTVAVGQEGLETPEGQYNIEAKEENPTWHVPESDWAG
                     SLAGQSIPPGPSNPIKARWMAIYEGAGIHGTEETSSLGTAASHGCIRMAIPDVEELYD
                     LVEVGTPIYIG"
     gene            2029..>2475
                     /locus_tag="VFB52_05515"
     CDS             2029..>2475
                     /locus_tag="VFB52_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZO05824.1"
                     /translation="MRSKDEVRKEVWKAMDREKVSRFPGAEGRIPNFAGAKLAAEKLA
                     AHRLWKRAQVIKANPDSPQTHARRIALDEGKTVIMAVPRLRDPHPFRVLDPKVLSKAA
                     LKEAATIKGALKHGRVIALDDLPEVDFVLCGSVAVNLSGARVGKGGG"
BASE COUNT          373 a          828 c          834 g          440 t
ORIGIN      
        1 catgacctaa tagctcttgg atcgtacgca gatcggcgcc accctccaag agatgcgtcg
       61 caaaagaatg tctaagtgaa tgcggcgaga ccccggccgc gagcgccgct tcgcgggtcc
      121 agaggccgag gcggcgggtg acgtcggagt tggagaggcg acggccgctc ttcgagagga
      181 acagcgcctg ctcgcgcggc tcggtggcca gcgcgcgtcg gcccttctgc aggtagcgct
      241 ccagtgcccg ctgggccggc tcgccgatcg gcaggatccg ctctttggat cccttgccga
      301 gcacccgcac ctgctcgctc tcgaagtcga ccgcgccgac gtcgaggttg acgatctcct
      361 cgcagcgcag gccacaggag taggcaagct ccagcatcgc ccggtcgcgc aactccagcg
      421 gcgtaccggc cgggatccgc tccagcagcg agcgcatctg ctcggtgctg agcacgctcg
      481 gcagcttttc cgcccgcttc gggctggaga ccagctcggc ggggttggag ccggcgcgct
      541 cggtgcgcac gaggaagccg tagaggccgc ggatcgccgc cagcttgcgg gccaccgtgg
      601 ccggcgcggc gccggcggtc gagagcccgg cggcgtagcg gcgcacgtcg cggtgacgca
      661 cgtcggtcgg ccccagcccc tggggcccca cccactcgac aaagccgccg aggtcgacgc
      721 cgtaggcgcg ccgggtccgc tcggcggagc cgcgggcccg cagatcgcgg tcgtaatcgg
      781 cgagggctga atcccagttc acgaagggga gttcgcccaa acccccctac gtcctgcgcg
      841 agctaaccga tgtaaatggg cgttccgact tcgacgaggt cgtacagttc ttcgacatcc
      901 gggatcgcca tgcggatgca gccgtgcgaa gcggcggtcc caagcgagga agtttcttcg
      961 gtgccgtgga tcccggcgcc ttcgtagatc gccatccagc gcgccttgat cgggttcgag
     1021 gggccgggcg ggatcgactg cccggccagc gaaccagccc agtcggattc cggcacgtgc
     1081 caggtcgggt tttcttcctt ggcctcgatg ttgtactggc cttcgggcgt ttccaggcct
     1141 tcctggccga ccgcgacggt gtaggtcttg gtgttcttga ggtcctgcca gaggcgcagg
     1201 gtgaaggtcg agcggtcgag ggtcaggtag gccgggtact ccttcgcgac ctccttggtg
     1261 gtcacgtcgg gcttcgtctt gtgcacgtgg gcgacaaccg tgcgcggcgc gctggcgttc
     1321 agcaccgcgt cttcgaggtc cttggtcagc tcgttgtcgc gcagcttcag accggccttg
     1381 ccggagacga cgttgagcga atcggagctc ggttcgacgc tggcgtcgac cgggtcgcgg
     1441 ttgacttctt cggcgacgtg gcggacgaag cggttgatcg ccgacttcga gtagtcgagt
     1501 tccgcggtca ccggcttgtc gagctcactg ccgctgacgt agcgaaccag gcgggtcggc
     1561 aggccaccgt cctggctctc ttcgaccgcc tcttcgaccg cctcgtcgat gttggcgcgg
     1621 atcttcagct tgtcgccggg cagcgtccag ctctcgtcgt ctagctcgac cttgagcgtg
     1681 tccttgagcg gcgccagcag ctcgcgctgc acctcggcct tcgcttcgtc ggcgtccatc
     1741 ccgccgacgt cgacgccggc gatcgtcaca ccctcggcga tcttgtcctt ctgggtgctg
     1801 tcgtagacgt aggcacccgc cgccaggacc acgatcagtc cgacgatcac tcccagcgtg
     1861 attttggtct tacgtcccat aacaggtgtc gatcccggat tcggtcgttc ccaaacccgc
     1921 ttcgaagagt ctaggcatct ggatcattcg gccccggaac ttgtcttccc tgcaatttcg
     1981 catctgcgtc ggtcttatcg gcagacgaga cggatatctt tcgccctaat gcgctcgaag
     2041 gacgaagtcc gcaaagaggt ctggaaggcg atggatcgcg agaaggtttc gcgattcccc
     2101 ggcgcagagg ggcggattcc caatttcgcc ggcgccaagc tggccgccga gaagctcgcc
     2161 gcgcaccggc tctggaagcg cgcccaggtg atcaaggcca acccggactc gccgcagacg
     2221 catgcgcgcc ggatcgccct cgacgaaggc aagaccgtga tcatggcggt gccgcgcctg
     2281 cgcgacccgc acccgttccg ggtgctcgac ccgaaagtgc tctcgaaagc ggcgctcaag
     2341 gaggccgcga cgatcaaggg cgcgctcaag cacggccgcg tgatcgcgct cgacgatctg
     2401 cccgaggtcg acttcgtgct ctgcggctcg gtcgcggtca acctcagcgg agcccgggtc
     2461 ggcaaaggcg gcggc
//
LOCUS       DASRIG010000200         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2724656, whole genome shotgun sequence.
ACCESSION   DASRIG010000200 DASRIG010000000
VERSION     DASRIG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724656"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFB52_05520"
     CDS             <1..426
                     /locus_tag="VFB52_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05825.1"
                     /translation="VSGQVGEIEIEGAGAGGSAWQTLELISLILMLTIAVTVGAAILR
                     LSGSEWKPAIAPGAAVAVLGGLSTLLIVFRIVVPPDLGELGGIEVKATLGLGAFLALC
                     AAVGIAYGGYRAMREEGSSFEAVADSLQPRRERPASRKR"
     gene            complement(341..2197)
                     /locus_tag="VFB52_05525"
     CDS             complement(341..2197)
                     /locus_tag="VFB52_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZO05826.1"
                     /translation="MAPGIDGLPAGERDVDVSGPVARFLATTSPRVLTRLRLGLRAFE
                     WLPFPWRFSRLDPVAAEDFLHKLEGSRLSFHHDLLLMAKLFSTLGYAVTPQVQERIGF
                     KIGCGLDDGTLPEPAGSLGDTEPQGEGEECDVVIVGSGAGGAVAATSLAEAGLDVLVL
                     EAGGHYNRENYPSDHLEAISTLYRDAGLTIAEGRPPIPVPVAKVVGGTTVINSGTCFR
                     APEPILDDWRTRFGIEWAHDLEADFAEAEEMLHVKQLDPNRMGRNGQLAMEGAQALGV
                     SAAPIFRNAGNCVQCSSCPFGCEIDAKRGMHVSYLPRAVAAGARVRAGVDVRRVLTEG
                     GRAVGVEASANGQPYTVRARRAVIAAGGALGTPELLQRSGLGGHHVGRNLHIHPACWV
                     GARYEEEVRGWDGVMQSFYVDQWEHEKILLEATFTPLAFGGAWLLGAGAEHQRRMLDF
                     AHVGSIGVHLCDSSAGRVGLGAEGSLRASYKLTNDDARRIAFGIARAAEVHFAAGASE
                     VYPNIARAQVLKPGDLANFEATRFKPSELRLEAFHPMGTARIAADPNDGACDLRGAVH
                     GVRDLYVADGSLFPTSVGVNPMMTIIAFAKQVARGVAGESPQQPQTSSLLHA"
     gene            complement(2258..2701)
                     /locus_tag="VFB52_05530"
     CDS             complement(2258..2701)
                     /locus_tag="VFB52_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05827.1"
                     /translation="MQGPAAERFVVLNLRLLAATFAVVGILFIAVPSGVLDVISDVGE
                     WFGNDTRAPHTQEYLWLALAFAYMVVITGICLLAQTDVVRYRALILVLAAGKTASSLG
                     SLAFFIVQDQVFIYLLNFLVDGFLAVFALVLWSIAGRIGRPLDPG"
     gene            2777..3388
                     /locus_tag="VFB52_05535"
     CDS             2777..3388
                     /locus_tag="VFB52_05535"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HZO05828.1"
                     /translation="MGFTRAELEEYRDHEVDDLVGPGLKLLFVGINPGLWTAAVNTHF
                     AHPVNRFYPALALAGISAYEIDRAAGMSDADRADLIARGVGITNLVRRATARADELSR
                     EELRAGGERLLRFVAEHRPKVVAIAGITAYRDAFGERGAKAGRQPETLESAARWSGAA
                     LWVVPNPSGLNAHETVATLAAAYAAPAAEAGVISPARSIESSP"
     gene            3385..3672
                     /gene="gatC"
                     /locus_tag="VFB52_05540"
     CDS             3385..3672
                     /gene="gatC"
                     /locus_tag="VFB52_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZO05829.1"
                     /translation="MIDREQVLHVAKLARLKLSDEEVERMTGELSHILDHVDRISKMD
                     LEGVEPTSHVVALENVLRADVPRPSLPREAALANAPEPVDGAFRVPSPQAE"
     gene            3672..>4355
                     /locus_tag="VFB52_05545"
     CDS             3672..>4355
                     /locus_tag="VFB52_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO05830.1"
                     /translation="MAGLLELNVAEAAAKIAAGEVSSEEYFAAWMAAAAGDELNAYLW
                     SVENADDYRVTEGPLSGIPVAVKDIFATEGSPTTAGSKILEGYTPPYTAHAVRQLDAA
                     GARVLGKTNMDEFAMGSSNENSAYGPVLNPWDRGRVPGGSSGGSAAVVAGGLAPCAIG
                     TDTGGSIRQPAALCGIVGLKPTYGSISRYGMIAFASSLDQCGPLTRDVTDAALMLAAL
                     EGRDPCDSTS"
BASE COUNT          627 a         1558 c         1577 g          593 t
ORIGIN      
        1 gtctccggcc aggtcggcga gatcgagatc gaaggggcag gagccggcgg cagcgcctgg
       61 cagacgctgg agctgatctc gctgattctg atgctgacga tcgcggtcac ggtcggggcg
      121 gcgatcctgc gcctcagcgg ctccgagtgg aagcccgcga tcgcccctgg cgccgcggtc
      181 gcggtgctcg gcggcctctc gacgttgctg atcgtcttcc gcatcgtcgt cccacccgac
      241 ctcggtgagc tcggcgggat cgaggtcaag gcaacgctcg gcctcggcgc cttcctcgcc
      301 ctctgcgccg cggtcgggat cgcctacggc ggctaccggg ctatgcgtga agaagggagc
      361 tcgtttgagg ctgttgcgga ctctctccag ccacgccgcg agcgacctgc ttcgcgaaag
      421 cgatgatcgt catcatcggg ttgaccccga ccgaggtggg gaagagcgag ccgtcggcga
      481 cgtagaggtc gcggaccccg tgcacggcgc cgcgcaggtc acaggcgccg tcgttcgggt
      541 cggctgcgat ccgcgccgtg cccatcgggt ggaaggcctc caggcgcagc tctgagggct
      601 tgaaccgggt cgcctcgaag ttcgccaggt cgcccggctt cagcacctgc gcccgggcga
      661 tgttcgggta gacctcgctc gccccggcgg cgaagtggac ttcggcggcg cgggcgatcc
      721 cgaaggcgat ccggcgggcg tcgtcgttgg tcagcttgta gctggcgcgc agcgagcctt
      781 cggcgccgag cccgacccgg cccgcggagc tgtcgcagag gtggacgccg atcgagccga
      841 cgtgggcgaa gtcgagcatc cgccgctggt gctcggcgcc ggccccgagc agccaggcgc
      901 cgccgaaggc gagcggcgtg aaggtcgcct cgagcaggat cttctcgtgc tcccactggt
      961 cgacgtagaa gctctgcatc accccgtccc agccgcgcac ctcctcctcg tagcgggcgc
     1021 cgacccagca ggcggggtgg atgtggaggt tgcggccgac gtggtggccg ccgagcccgg
     1081 agcgctgcag cagctccggc gtgcccagcg ctccgccggc ggcgatcacc gcgcgccgcg
     1141 cgcgcacggt gtagggctgg ccgttggcgc ttgcttcgac cccgaccgcg cggccgccct
     1201 cggtcagcac ccggcgcacg tcgacgccgg cgcgcacccg cgccccggcg gcgaccgccc
     1261 gcggcaggta gctgacgtgc atcccgcgct tggcgtcgat ctcgcacccg aacgggcagg
     1321 agctgcactg cacgcagttg ccggcgttgc ggaagatcgg cgcggcggaa acacccagcg
     1381 cctgcgcgcc ttccatcgcc agctggccgt tccgccccat ccggttcggg tcgagctgtt
     1441 tgacgtgcag catctcctcg gcctcggcga agtcggcttc gaggtcgtgc gcccactcga
     1501 tcccgaagcg ggtgcgccag tcatcgagga tcggctccgg cgcccggaag caggtgcccg
     1561 agttgatcac cgtggtgccg ccgaccactt tcgccaccgg caccgggatc ggcggccgac
     1621 cttcggcgat cgtcagcccg gcgtcgcggt agagggtgga gatcgcttcc aggtggtcgc
     1681 tcgggtagtt ctcgcggttg tagtggccgc cggcctcgag cacgagcacg tcgagcccgg
     1741 cctcggccag gctggtcgcc gcgaccgcgc cgccggcgcc cgagccgacg atcaccacgt
     1801 cgcattcctc gccctcgccc tgcggctcgg tgtcgccgag cgagccggcc ggctcgggca
     1861 gggtgccgtc gtcgagcccg cagccgatct tgaagccgat ccgctcctgc acctgcgggg
     1921 tgaccgcgta gccgagggtc gagaacagct ttgccatcag cagtagatcg tggtggaagg
     1981 agaggcgcga gccctcgagc ttgtgcagga agtcctcggc ggccacgggg tcgaggcggc
     2041 tgaaacgcca ggggaagggc agccactcga aggcgcgcag cccgagccgc agccgggtca
     2101 gcacgcgcgg ggaggtggtg gcgaggaagc gggcgaccgg gccggagacg tcgacgtcgc
     2161 gctctcccgc gggcaggccg tcgattcccg gcgccatcgc cgcgcagatc gcgctcaggg
     2221 tgcgttgctc agcggccgag agccctggcg ggcgccttca cccgggatcg agcgggcggc
     2281 cgatccgccc ggcgatcgac cagaggacca gcgcgaagac ggcgaggaag ccgtcgacga
     2341 ggaagttgag caggtagatg aagacctggt cctggacgat gaagaaggcg agcgagccga
     2401 gcgaggaggc cgtcttgccg gcggcgagga cgaggatcag cgcccggtag cggaccacgt
     2461 cggtctgcgc gagcaggcag atcccggtga tcacgaccat gtaggcgaag gcgagcgcca
     2521 gccagaggta ctcctgcgtg tgcggcgccc gggtgtcgtt gccgaaccat tcgccgacgt
     2581 cgctgatcac gtcgagcacg ccgctgggca ccgcgatgaa gaggatcccg acgaccgcga
     2641 aggtcgccgc gagcaggcgc aggttcagca ccacgaatct ctcggcggcc ggtccctgca
     2701 tcctgccggg acaatactgg ctgggcagcc agtacgccag cggacggcaa ccgcgccgtt
     2761 ccgaaaggat cggggcgtgg ggttcacgcg cgccgagctg gaggagtacc gcgaccacga
     2821 ggtcgacgac ctggtcgggc cggggctgaa gctgctgttc gtcgggatca accccgggct
     2881 gtggacggcc gcggtcaaca cccacttcgc ccacccggtc aaccgcttct acccggcgct
     2941 ggcgctggcc gggatctccg cctacgagat cgaccgcgcc gccgggatga gcgacgccga
     3001 ccgcgccgac ctgatcgccc gcggggtcgg gatcaccaac ctggtgcggc gggcgaccgc
     3061 gcgcgccgac gagctctcgc gcgaggagct gcgcgcgggc ggcgagcggc tgctccggtt
     3121 tgtcgccgag caccggccca aggtggtcgc gatcgccggc atcaccgcct accgcgacgc
     3181 cttcggcgag cgcggcgcca aggcggggcg ccagccggag acactcgaaa gcgccgcccg
     3241 ctggtccgga gcggcgctct gggtggttcc caacccgagc ggtctgaacg cccacgagac
     3301 ggtcgcgacg ctcgccgccg cctacgccgc gcccgccgcc gaggcaggcg tgatctcccc
     3361 agcccgatcc atagaatctt cgccgtgatc gaccgcgagc aggtactcca cgtcgccaag
     3421 ctggcgcgcc tgaagctctc cgacgaggaa gtcgagcgga tgaccggcga gctttcgcac
     3481 atcctcgacc acgtcgaccg gatctcgaag atggacctcg agggggtcga gccgacctcg
     3541 cacgtggtcg cactggagaa cgtgttgcgc gccgacgtgc cgcgtcccag cctgccccgc
     3601 gaggcggcgc tcgccaacgc tcccgaaccg gtcgacggcg ccttccgcgt cccctcgccg
     3661 caggccgaat gatggccggc ctgctcgagc tcaacgtcgc cgaggcggcg gcgaagatcg
     3721 ccgccggtga ggtctcgagc gaggagtact tcgccgcctg gatggcggct gcggccggcg
     3781 acgagctgaa cgcctacctg tggtcggtcg agaacgccga cgactaccgg gtcaccgagg
     3841 gcccactgag cgggatcccg gtcgcggtca aggacatctt cgccaccgag ggatcgccga
     3901 ccaccgccgg ctcgaagatc ctcgagggct acacgccgcc ctacaccgcg cacgcggtgc
     3961 gccagctcga cgccgccggc gcccgcgtgc tcggcaagac caacatggac gagttcgcga
     4021 tggggtcctc gaacgagaac tccgcctacg ggccggtgct gaacccctgg gaccgcggcc
     4081 gcgtccccgg cggctcctcg ggcggctcgg ccgccgtcgt cgccggtggg ctcgccccct
     4141 gcgcaatcgg caccgacacc ggcggctcga tccgccagcc cgcggcgctc tgcgggatcg
     4201 tcggcctgaa gccgacctac ggctcgatct cccgctacgg gatgatcgcc ttcgcctcct
     4261 cgctcgacca gtgcgggccg ctgacccgcg acgtcaccga cgcggcgctg atgctggcgg
     4321 cgctggaggg gcgcgacccc tgcgactcga cctcg
//
LOCUS       DASRIG010000201         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1916081, whole genome shotgun sequence.
ACCESSION   DASRIG010000201 DASRIG010000000
VERSION     DASRIG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916081"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFB52_05550"
     CDS             complement(<1..966)
                     /locus_tag="VFB52_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO05831.1"
                     /translation="MSANTTYDVAIVGGGHNGLVAAYYLAREGLRVVVCERREIVGGC
                     CVTEEFAPGYRASTGAYVLSMLREPIWKDMRLVERGIVVDPAGPSLHLFADGTRLLLS
                     EETAAAQAAVRELSAADAAALAKFEDDLAEIAQLVTPLIDETPPDVRPRHPGELGSLL
                     KLGGLAARKRALINEALFLFSTSVTQFLDERFESDHVKAALGWHAINDSISGPSTPGT
                     AYILLHDHASEDAGGGARSWGFVRGGIGRVTEAMADAAREAGAEIRTEAEVERVLVAG
                     DRAVGLELADGSELRAARVLSNADPKKTFLGMLDAGELPAGFRASV"
     gene            complement(963..1868)
                     /locus_tag="VFB52_05555"
     CDS             complement(963..1868)
                     /locus_tag="VFB52_05555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05832.1"
                     /translation="MEASTATPAALTVAGERPSRFSRLLRPLAFMSLPSAALIVLFLI
                     PMGILAIYSFEYGDLTGSSGFTTTNFTDILGDSLYGEIALTTLEIATIAMLAQLIVGL
                     PLAYVLAYKSGKFELPLLLALVLADELNPMVKIYAWRMLLGREGLINEALTSIGLISQ
                     PIDALLFNKFAVIVVLSTSYLTYTVIPIYASMKAVDAGLFEAARDLGAGWFTTFRRVL
                     LPLIAPGIFIALLLVYVPLFTDFAAPTLVGGTDGYMLGNVVSDLVLENGNLNAGAAMS
                     FLMLIGAGLFSLVAYQLTRIKSGQK"
     gene            complement(1869..2726)
                     /locus_tag="VFB52_05560"
     CDS             complement(1869..2726)
                     /locus_tag="VFB52_05560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05833.1"
                     /translation="MPLSRRLSRSLPNALSIALVVFVLAILFGPVLLLALFSFNDSTV
                     ISLPWEGFTTKWYSEAFNEEAATTAILNSVKVATIVMAVSLVLGTLAAWGLTRIPFRG
                     RALVAGVHGAVLVVPWLIIGVAGLIFFSQIEWELSLTTIVLMHIVVTFPLVVAIVSAG
                     LVRFNRSIEEAAIDLGASQWQMLRYVVLPQIAPSLAASAIFAFAWSFNNFEISFFTGG
                     FEQTFPVWVFGVLRHSSNLPIVNAASTVIAAVQVVVVFALWYGMKLMTRRRGGTDQDM
                     AEMMTGAVR"
     gene            complement(2747..>2950)
                     /locus_tag="VFB52_05565"
     CDS             complement(2747..>2950)
                     /locus_tag="VFB52_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05834.1"
                     /translation="LNYYASPEAQAISATNGFVVTNPDAIPLVPKQYRETADPASIKG
                     AIPLTEPEDVDAYTRAWQEVRSG"
BASE COUNT          500 a          958 c         1078 g          414 t
ORIGIN      
        1 caccgaggcg cggaacccgg ccggcagctc gcccgcgtcg agcatgccaa ggaaggtttt
       61 cttcgggtcg gcgttggaga gcacccgcgc agcgcgcagc tcgctgccgt cggcgagctc
      121 gaggccgacc gcgcgatcgc ccgcgaccag gacccgctcg acctcggcct cggtgcggat
      181 ctccgccccg gcctcgcgcg ccgcgtcggc catcgcctcg gtcacgcgcc cgatcccgcc
      241 gcgcacgaag ccccaggagc gggcgccgcc gccggcgtcc tcggaggcgt ggtcgtggag
      301 caggatgtag gcggtgccgg gggtcgaggg gccggagatc gagtcgttga tcgcatgcca
      361 gccgagcgcc gccttcacat ggtcgctttc gaaccgctcg tcgaggaact gggtgaccga
      421 ggtgctgaag aggaagagcg cctcgttgat caaggcgcgc ttgcgcgccg ccaggccgcc
      481 gagcttgagc agcgagccga gctcgcccgg gtggcgcggg cgcacgtcgg gcggggtctc
      541 gtcgatcagc ggcgtgacca gctgcgcgat ctccgccagg tcgtcctcga acttggccag
      601 tgccgcggcg tcggcggcgg agagctcgcg gaccgccgcc tgcgccgccg cggtctcctc
      661 ggagagcagc agccgggtgc cgtcggcgaa aaggtgcagg gaggggccgg cgggatcgac
      721 gacgatcccg cgctcgacca ggcgcatgtc cttccagatc ggctcgcgca gcatgctgag
      781 cacgtaggcg ccggtcgagg cgcggtagcc gggcgcgaac tcctcggtca cgcagcagcc
      841 gccgacgatc tcgcgacgct cgcagaccac cacccgcagg ccctcgcgag ccaggtagta
      901 ggcggcgacg aggccgttgt ggccgccgcc gacgatcgcg acgtcgtagg tcgtgttggc
      961 gctcacttct gccccgactt gatcctggtg agttggtagg cgaccaacga gaacaggccc
     1021 gcgccgatca gcatcaggaa gctcatcgcc gcgcccgcgt tgaggttgcc gttctccagg
     1081 accagatcgc tgaccacgtt gccgagcatg tagccgtcgg tgccgccgac cagggtcggg
     1141 gcggcgaagt cggtgaacag cggcacgtag accagcagca gggcgatgaa gatccccggc
     1201 gcgatcagcg gcagcagtac ccggcggaag gtcgtgaacc agccggcgcc gaggtcgcgg
     1261 gcggcctcga agaggcccgc gtcgacggct ttcatcgagg cgtagatcgg gatcacggtg
     1321 taggtcaggt agctggtcga gagcaccacg atcaccgcga acttgttgaa cagcagggcg
     1381 tcgatcggct ggctgatcag cccgatcgag gtcagcgcct cgttgatcag cccctcgcgg
     1441 ccgagcagca tccgccaggc gtagatcttc accatcgggt tgagctcgtc ggccaggacc
     1501 agggccagca gcagcggcag ctcgaacttg cccgacttgt aggcgagcac gtaggcgagc
     1561 ggcaggccga cgatcagctg cgcgagcatc gcgatcgtcg cgatctccag cgtggtcagg
     1621 gcgatctcgc catagagcga gtcgccgagg atgtcggtga agttggtggt ggtgaagccg
     1681 gagctgccgg tcaggtcgcc gtattcgaag ctgtagatcg cgaggatccc catcgggatc
     1741 aggaagagca cgatcagcgc cgcgctcggc agcgacatga aggcgagcgg gcgcagcagg
     1801 cggctgaacc gcgagggacg ctcgccggcg acggtcaacg ccgcgggggt tgcggtcgag
     1861 gcctccacct agcgcaccgc cccggtcatc atctcggcca tgtcctggtc ggtgccgccg
     1921 cggcgccggg tcatcagctt catcccgtac cagagcgcga acacgacgac gacctggacc
     1981 gcggcgatca cggtcgaggc ggcgttgacg atcggcaggt tcgaggagtg gcggagcacg
     2041 ccgaaaaccc agaccgggaa ggtctgttcg aagccgccgg tgaagaagct gatctcgaag
     2101 ttgttgaacg accaggcgaa ggcgaagatc gccgaggcgg ccagcgaggg cgcgatctgc
     2161 ggcaagacga cgtagcgcag catctgccac tgcgaggcgc cgaggtcgat cgccgcctct
     2221 tcgatcgagc ggttgaagcg gaccaggccg gcggagacga tcgcgacgac cagcgggaag
     2281 gtgacgacga tgtgcatcag cacgatcgtg gtcagcgaga gttcccattc gatctggctg
     2341 aagaagatca gcccggcgac gccgatgatc agccagggca cgaccagcac cgccccgtgg
     2401 accccggcga ccagggcgcg gccgcggaac gggatccggg tcagccccca ggcggccagc
     2461 gtgcccagca ccagcgagac cgccatcacg atcgtcgcca ccttgaccga gttgaggatc
     2521 gccgtggtcg ccgcttcttc gttgaacgcc tccgagtacc acttggtcgt gaagccctcc
     2581 cagggcagcg agatcacggt cgagtcgttg aacgagaaca gcgccagcag cagcaccggg
     2641 ccgaacagga tcgccagcac gaacacgacc agcgcgatgc tgagcgcgtt cggcaggctg
     2701 cggctgaggc ggcgggagag tggcatggtg gcgacctgct ccgggatcag ccggagcgga
     2761 cttcctgcca ggcgcgggtg taggcgtcca cgtcttcggg ctcggtcagc gggatcgcgc
     2821 ctttgatgct ggccgggtcg gcggtttcgc ggtactgctt cgggaccagc gggatcgcat
     2881 cggggttggt gacgacgaag ccgttggtcg cggagatcgc ctgcgcctcc gggctcgcgt
     2941 agtagttgag
//
LOCUS       DASRIG010000202         9931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1403215, whole genome shotgun sequence.
ACCESSION   DASRIG010000202 DASRIG010000000
VERSION     DASRIG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403215"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFB52_05570"
     CDS             complement(<1..593)
                     /locus_tag="VFB52_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZO05835.1"
                     /translation="MIRAGIVVTGTEVITGRISDSNGPWVSERLAELGVEVAHILVVA
                     DRPDDLEAALRFLAEEGMDLIVTTGGLGPTADDLTAEVVGKFAGRELVLDEEMLEKIA
                     EILRAFARRRAFNIDEEAMLEANRKQAMVPEGAEAIDPVGTAPGLVVPAGDRVVIVLP
                     GPPRELQPMWPIALEAPAVREILDRSTPLHGYTMRMFG"
     gene            complement(636..2876)
                     /locus_tag="VFB52_05575"
     CDS             complement(636..2876)
                     /locus_tag="VFB52_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZO05836.1"
                     /translation="MFVRQKRSAPAKKSKAKGRKKAKAAKPARAGLLDRFEQRHLDLI
                     GVGMLAAGLFLLFVLFFGWEGGKVGYGLKTGLEYLGGEVGAKIFTLLLLLVGGMLLTG
                     TSVSMLATGIGRGLRAVFRAIFFSGTEAAQTVARHHDDWRERREDRASEKETDVMASY
                     PDEDDDDFEPTVALADDDDRFAPGLFDIDSDAEAETVVEAAEEDPEPEEEPQQELRNY
                     DPADAVAVVDEGDRAHAGLTPLGNKRGVTMSEEIDYRPPPAKALARGKGDKGPDPRDQ
                     EAVGRKLIETLAHFRVDAKIVGVVSGPHVSLYELRLAPGIKVKKVTELANDLAYALAS
                     TDIRILAPIPGKQAVGVEVPNSKRRMVTLGDIYAGRPAKTSPLVAWLGKGIDGNAVWT
                     DLAKMPHVLVAGTTGSGKSGCVNAILSSILMQASPNDVRLVLVDPKQVELNHYENVPH
                     LLTPVVTSPRLAANVLSNLIGEMESRYGIMSQARTRNLDELNRVRIKSGEAPLPHILC
                     VIDELADLMMVAPAEVEDSIIRLAQKSRATGIHLVLATQRPSTDIITGTIKVNIPSRI
                     AFAVSSQTDSRVILDQGGAESLLGQGDMLFRGPGSSKLQRIQGAFITEDEIAKITNHW
                     AKQGEPEFEDELLETPVEAEEEGRGDDDLDPDSDDLLDEAIRLVVQTETASVSMIQRR
                     LRVGYTRAGRLIDMLERRGVISGYEGSKPRQVLISQADLGRVLSPAGPASDSGPVATA
                     EPDNDA"
     gene            3003..3695
                     /locus_tag="VFB52_05580"
     CDS             3003..3695
                     /locus_tag="VFB52_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO05837.1"
                     /translation="MLSEAEQTGGRVLVVEDDQDIADVLRRSLRNEGYEVRTSGDGIE
                     ALDVAAGFMPDLVVLDLGLPGLDGVEVCRRLRSDGDVPILMLTARSETEDRVEGLDSG
                     ADDYLVKPFERQELLARIRALLRRRPPRGAASLEVGDLQLNPDTREVRRGEREIELTN
                     REFELLEFLMRNERLVVSRERLLDEVWGYDPMAATNTIDVFVSNLRRKLEAGEEPRLL
                     HTKRGAGYVLKE"
     gene            3699..5168
                     /locus_tag="VFB52_05585"
     CDS             3699..5168
                     /locus_tag="VFB52_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO05838.1"
                     /translation="MATAKKRTGAPLRRLVHPRSWPVRWRLSAVSAALTFLILLMFGG
                     AIGQIATQRIRDDFNNEVRSAVQILAGQVRIVYPVLGEPRAEIGPQLNAFVLPDDASA
                     RIFDIRGNLIEENRGAEPLGPPQEGLSDYQGKRVATEQVINEEGQPTGFVQYGRSLEH
                     VDSTVGRVWFLIVTGIIAGTVLAILAGVAIASRAMRPVSSLTATAREIAMTRDPSQRM
                     PEPPVDDEVGELADTLDQMLRSLDEARGERESAMQRQREFVADASHELRTPLTSVLAN
                     LELLQASMQQAGQAEDREIVDSALRSSKRMNRLVGDLLLLARADAGRLGTHRRCDLAE
                     IAGEAAAEAAPLLGERELVIDNDRPLRVEGNADELHRLVLNLLDNAAHHTPEDARIEL
                     TLREDGNDAVVEVADNGAGIPAEVRDQIFGRFVRGVGPADTNAGTGTGLGLAIVSAVA
                     ASHGGSVIVSDSSSGGALFRARIPMGAPQPAKSNLLEPL"
     gene            complement(5741..7405)
                     /locus_tag="VFB52_05590"
     CDS             complement(5741..7405)
                     /locus_tag="VFB52_05590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZO05839.1"
                     /translation="MSNKLRVLPLGGLGEIGKNMTVIEYDGKIVVVDTGLMFPTAEMH
                     GIDLVLPDFSYLRDRADDIEAIVLTHGHEDHVGALPYVLREIGAPPVIYGGLLTIGMV
                     RSKLDEHKLGEAAPLEELPPGQKVQRGPFELELVHLAHSIPDMRGVLIKTKAGSVLMT
                     GDYKFDQTPVDGKPADVSRLAEIGREGLTLLCGDSTNADRPGVAPSESSVGPALLQEF
                     SRCEGRIIVTSFASNIHRVQQVIDAAVELDRKVALVGRSMRKNFNIASNLGIAQAPQG
                     IFIQPREIEDFPDDKVVVISTGSQGEPLSALRRMANNDHRDVQLHSGDTVVFSATPVP
                     GNERAVNETIDRIYEIGATVITAKDAPIHASGHGWQEELKLMLNLTKPQFVMPVHGDH
                     KRLRLHSELAQAVGVPAEHVFQGRNGLPLEVDADGASFGEDIHAGVIYVDGVNIGEPD
                     DAALRDRREIAADGVFIVVATISADDGSIVADPEVIFRGVAFLEEAGDLIEDLSDLVE
                     DSLEDAAKAGNRKIDLLQEDLHDEIGKFVFQRLRRRPMVLPVVVEV"
     gene            complement(7451..8326)
                     /gene="dapA"
                     /locus_tag="VFB52_05595"
     CDS             complement(7451..8326)
                     /gene="dapA"
                     /locus_tag="VFB52_05595"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZO05840.1"
                     /translation="MASIEGAITAMATPFREDGTVDLDGARRLASHLLEHGSHGLVIA
                     GTTGECPTLTDGETIEIFRAVRAEVGDEVLLICGTGTNDTQHSRELTKMAADAGADGS
                     LVVVPYYNKPNRAGIRAHFEAVAEAVPELPMVMYNIPARVIVNADPELLGELGRDVDN
                     IVAVKQANDEDLGPIDGLKVLAGNDNTFLRVLEFGGAGGILVAAHVVGDQMREMWDAA
                     QANDLERARQIDAELTPVYEGLSVTTNPIPLKAALEMLGLAPDRMRLPMVPADSEQRA
                     AVRAALEGYGLPVAR"
     gene            complement(8376..9053)
                     /locus_tag="VFB52_05600"
     CDS             complement(8376..9053)
                     /locus_tag="VFB52_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZO05841.1"
                     /translation="MSPVPIKVGVSGAAGKMGQAVCEAVEGAADTELAGKADPTLGTS
                     LEDILGDCDVVVDFTIPDTALANVKACLAADVHVVVGTTGFDLDAAKAAAEASEANCF
                     VAPNFAIGAVLMMMVAQTIAPHMPECEVIELHHDKKLDAPSGTAKRTAELIDAAGGNV
                     HQPIHSVRLQGFVAHQEVIFGGEGQTLSIRHDSIDRRSFMPGVLLAVRKVGELPEPFT
                     VGLERLL"
     gene            complement(9050..>9931)
                     /locus_tag="VFB52_05605"
     CDS             complement(9050..>9931)
                     /locus_tag="VFB52_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZO05842.1"
                     /translation="EDEPQDRVHDVLAEAVFGQHPLGRRVLGEAEVTASIPIPDIDAY
                     HRERYAGREIVVGAAGHLDHDRIVALAEKLVNPTGAGGDQPDMELDGGAQLCFHQKET
                     EQYHICFGAPGLRRDDERRYALAVLDSIFGGSTSSRLFREVREKRGLAYSVGSYNEQF
                     TDQGLVATYVGTREDNVEEACSIIGTELARLRSEPVSAEELTRAKESVKGRLVLSSES
                     TAARMTRISRATLFGLPIESLDEMLARVDAIAVDDLTELAAELYAEDRLSAACVGADE
                     DRFRAALAPVSETLVAA"
BASE COUNT         1483 a         3363 c         3457 g         1628 t
ORIGIN      
        1 ccgaacatcc gcatcgtgta gccgtgcagg ggcgtcgagc ggtcgaggat ctcgcgcacc
       61 gccggcgcct ccagcgcaat cggccacatc ggctgcagct cgcgcggtgg gcccggcagc
      121 acgatcacga cgcggtctcc cgccggcacg accaggccgg gcgcggtccc gaccgggtcg
      181 atcgcctcag cgccctcggg gaccatcgcc tgcttgcgat tggcctccag catcgcctcc
      241 tcgtctatgt tgaaggcgcg ccggcgggcg aacgcgcgca ggatctcggc gatcttctcc
      301 agcatctcct cgtcgaggac cagctcgcgg cctgcgaact tgccgaccac ctccgcggtg
      361 agatcgtccg cggtcggccc caggccgccg gtggtgacga tcaggtccat gccctcctcg
      421 gcgaggaagc gcagcgccgc ttcgaggtcg tccggccggt cggcgacgac gaggatgtgc
      481 gcgacctcga cccccagctc ggccaggcgt tcggagaccc acggaccgtt ggagtcggag
      541 atccgccccg tgatcacctc ggtccccgtg acgactatcc cggcgcgaat cacgcgctga
      601 agctacatga cccgcggaaa aggaaccagg cggccctagg cgtcgttgtc gggctctgcc
      661 gtggcgaccg ggccggagtc gcttgccggc cccgcggggc tcagcacccg gccgaggtcg
      721 gcttgcgaga tcagcacctg gcgcggtttt gagccctcgt agccggagat cacgccgcgc
      781 cgctcgagca tgtcgatcag gcggcccgcg cgggtgtagc cgacccgcag gcggcgctgg
      841 atcatcgaga cggaagcggt ttcggtctgc acgaccaggc ggatcgcctc gtcgagcagg
      901 tcgtcgctgt ctgggtcgag gtcgtcgtcg ccgcgcccct cctcctcggc ctcgaccggg
      961 gtctccagca gctcgtcctc gaattccggt tcgccctgct tggcccagtg gttggtgatc
     1021 ttcgcgatct cgtcctcggt gatgaaggca ccctggatcc gctgcagctt tgagctgccg
     1081 ggaccgcgga agagcatgtc gccctgaccg agcagcgact cggcaccgcc ctgatcgagg
     1141 atcacccgcg agtcggtctg cgaggaaacc gcgaaggcga tccgcgaggg gatgttgacc
     1201 ttgatcgttc cggtgatgat gtcggtcgag ggtcgctgcg tcgccagaac gaggtggatg
     1261 ccggtggcgc gcgacttctg cgcgaggcgg atgatcgagt cctcgacttc ggccggggcg
     1321 accatcatca ggtccgcgag ctcgtcgatc acgcagagga tgtgcggcag cggcgcctcg
     1381 ccgcttttga tccgcacccg gttcagctcg tcgaggttgc gggtgcgggc ctggctcatg
     1441 atcccgtagc ggctctccat ctcgccgatt aggttggaca gcacgttggc cgccaggcgc
     1501 ggcgaggtga ccaccggcgt cagcaggtgc gggacgttct cgtagtggtt gagctcgacc
     1561 tgcttggggt cgacgaggac caggcgcacg tcgttgggcg aggcctgcat caggatcgag
     1621 gagaggatcg cgttcacgca gccgctcttg cccgagccgg tggtgccggc gacgagcacg
     1681 tggggcatct tcgcgaggtc ggtccagacc gcgttgccgt cgatgccctt gccgagccag
     1741 gcgaccagcg gcgaagtctt ggcgggacgg ccggcgtaga tgtcgccgag cgtgaccatg
     1801 cggcgcttgc tgttcgggac ctcgacgccg accgcctgtt tgccggggat cggggcgagg
     1861 atgcggatgt cggtcgaggc gagcgcgtag gcgaggtcgt tggccagctc ggtgactttt
     1921 ttgactttga tcccgggcgc gaggcgcagt tcgtagagcg agacgtgcgg accgctgacc
     1981 acgccgacga tcttcgcgtc gacccggaaa tgggccagcg tttcgatcag cttgcggccg
     2041 accgcttcct ggtcgcgtgg gtcggggccc ttgtcgcctt tgccgcgcgc cagcgctttt
     2101 gccggcggcg gccggtagtc gatctcttcg gacatcgtca cgccgcgctt gttgccgagc
     2161 ggggtgaggc cggcgtgggc gcgatcgccc tcgtcgacca cggcgacggc gtccgcgggg
     2221 tcgtagttgc gcagttcctg ctgcggctcc tcttccggct cggggtcttc ctcggcggcc
     2281 tcaaccaccg tctcggcctc ggcgtcggag tcgatgtcga agagccccgg ggcaaagcgg
     2341 tcgtcgtcgt ccgccagggc gaccgtcggc tcgaagtcgt cgtcgtcctc gtccgggtag
     2401 gaggccatca cgtcggtctc cttttcggag gcgcggtcct cgcggcgctc gcgccaatcg
     2461 tcgtggtggc gcgcgacggt ctgagccgcc tcggtcccgc tgaagaagat cgcgcggaag
     2521 acggcgcgca gcccgcgtcc gatcccagtc gccagcatcg agaccgaggt ccccgtcagc
     2581 agcatgccgc cgaccagcag caggagcagg gtgaagatct tggcgccgac ctcgccgccg
     2641 agatactcga ggccggtctt caggccatag ccgaccttgc cgccctccca gccgaagaag
     2701 aggacgaaga gcaggaagag ccccgcggcc agcatcccga ccccgatcag gtcgaggtgg
     2761 cgctgctcga agcggtcgag caggccggca cgagccggct tcgcggcttt ggcttttttc
     2821 cgccctttcg ctttcgattt tttggcaggg gcgcttcgct tctggcggac gaacatgtcc
     2881 aaccgggttc gacgccgccc ccctatcccc tgcgcgctcg acgcaaggcc gatcccgtcg
     2941 ccctaaattc gctctaaatc cccggtcgta ggcgagtttt ccggtctaac atcggtgcac
     3001 gcatgctgag cgaagccgaa cagaccggtg ggcgcgtcct cgtcgtcgag gacgaccagg
     3061 acatcgccga cgtgctgcgc cgctcactgc gcaacgaggg ctacgaggtc cgaacatcag
     3121 gtgacgggat cgaggcgctc gacgtcgccg ccggtttcat gcccgacctg gtcgtgctcg
     3181 acctcggcct acccggactc gacggggtcg aggtctgccg ccgcctgcgc agcgacggcg
     3241 acgtgccgat cctgatgttg accgcgcgct cggagaccga ggaccgggtc gaggggctcg
     3301 acagcggcgc cgacgactac ctggtcaagc cgttcgagcg ccaggagctg ctggcgcgga
     3361 tccgcgccct gctgcgccgc cgtccgcccc gcggcgccgc ctcgctcgag gtcggcgacc
     3421 tgcagctcaa ccccgatacc cgcgaggtgc gccgcggcga gcgcgagatc gagctgacca
     3481 accgcgagtt cgagctgctc gagttcctga tgcgcaacga gcgcctcgtc gtctcgcgcg
     3541 agcgcctgct cgacgaagtc tggggctacg acccgatggc ggcgacgaac acgatcgacg
     3601 tcttcgtctc caacctgagg cgcaagctcg aggccggtga ggagccgcgg ctgctgcaca
     3661 ccaaacgcgg cgccggctac gtgctcaagg agtaacgcgt ggcgaccgcg aagaagcgca
     3721 ccggggcgcc gctccgccgt ctcgtccatc cccgctcctg gcctgtgcgc tggcgcctct
     3781 ctgccgtctc ggcggcgctg accttcctga tcctgctcat gttcggcggc gcgatcggcc
     3841 agatcgcaac ccagcggatc cgcgacgact tcaacaacga ggtgcgcagc gcggtgcaga
     3901 tcctcgctgg gcaagtgagg atcgtctacc cggtgctcgg ggaaccgcga gcggaaatcg
     3961 ggccccagct gaacgccttc gtcctgccgg acgacgcctc cgcccggatc ttcgacatcc
     4021 gcggcaacct gatcgaagag aacagaggcg ccgaaccgct cggcccgccc caggaggggc
     4081 tttccgacta ccaaggaaag cgcgtcgcga ccgagcaggt catcaacgaa gaaggccagc
     4141 cgaccggctt cgtccagtac gggcgcagcc tcgagcacgt cgactcgacc gtcgggcggg
     4201 tctggttcct gatcgtgacc gggatcatcg ccggcaccgt gctcgcgatc ctggccggag
     4261 tcgcgatcgc cagccgcgcg atgcgtcccg tctcctcgct gaccgcaacc gcccgtgaga
     4321 tcgcgatgac ccgcgatccc tcgcagcgca tgcccgagcc gccggtcgac gacgaggtcg
     4381 gagaactggc agacacgctc gaccagatgc tgcgctcgct cgacgaggcg cggggagagc
     4441 gcgagagcgc gatgcagcgc cagcgcgagt tcgtcgccga cgcctcgcac gagctgcgca
     4501 ccccgctgac cagcgtgctt gccaacctgg agctgctgca ggcttcgatg cagcaggccg
     4561 gccaggccga agaccgcgag atcgtcgact cggcgctgcg ctcctcgaaa cggatgaacc
     4621 gcctggtcgg ggacctgctg ctgctcgccc gagccgacgc gggccgcctc ggcacccatc
     4681 gccgctgcga cctcgccgag atcgccggcg aagccgccgc cgaggcggca ccgttgctcg
     4741 gcgagcgcga gctggtgatc gacaacgacc ggccgctgcg ggtggaaggc aacgccgacg
     4801 agctgcaccg actcgtcctc aacctgctcg acaatgccgc ccaccacacc ccggaggacg
     4861 cgcggatcga gctgaccctg cgcgaggacg gcaatgacgc cgtggtcgaa gtcgccgaca
     4921 acggcgccgg gattccggcc gaggtgcgcg accagatctt cggccgcttc gtccgcgggg
     4981 tcgggccggc cgacaccaat gccggcaccg ggaccggcct cggcctcgcg atcgtcagtg
     5041 ccgtcgccgc ctcgcacggc ggcagcgtga tcgtcagcga ctccagttcg ggcggcgccc
     5101 tcttccgcgc ccggatcccg atgggggcac cccagccggc caaaagcaat cttttagagc
     5161 cgctttagac ccgctccgta cagtccttat tggacgagac acctgcccgc cggagcttct
     5221 ccccagacac tagctccggc gggctttttc tttggtgggt tgcagaggga cggcggggga
     5281 cggacggctg tcggtcccct ctccgggaat tcagtcgcat ccctatcggg atagctcccc
     5341 tagaaggaac ccgcctatgg cgggtcccga atcagattgg catttgggcc ttcggcacat
     5401 tcccggagag gggaccgaca cccgtcccac gctcgcagtc cctcgtaccc agaagtaaaa
     5461 ggcccgcctc ctacactagc tcccgtccat ctgacgctcg agatggaaac ggctcgggcg
     5521 gctaccgatt gacgccggtc ctgagcgctt cctggatttc ggggggctgt gattgggaag
     5581 ccgggtggtc tctgggggaa tgtggcccga agggccccga tggtatctgt ctctgggccc
     5641 gccataggcg ggctcctcat tcgggagcta cgacctttag gtcgtgcgac tcaattcccc
     5701 cagagaccac ccggcttccc ccaccgcgac ccccgaagga tcagacctcg acgacgaccg
     5761 gcagaaccat cggccgccgg cgcaggcgct ggaagacgaa cttgccgatt tcgtcgtgga
     5821 ggtcttcctg cagcaggtcg atcttgcggt tgccggcctt ggcggcgtcc tcgagggagt
     5881 cttcgaccag gtcgctgagg tcttcgatca ggtcacccgc ctcctcgagg aaggcgacgc
     5941 cgcggaagat cacctcgggg tcggcgacga tgctgccgtc gtcggcggag atcgtggcga
     6001 cgacgatgaa gacgccgtcg gcggcgattt cgcggcggtc gcggagggcg gcgtcgtcgg
     6061 gctcgccgat gttgacgccg tcgacgtaga tcacgccggc gtggatgtcc tcgccgaagg
     6121 aggcgccgtc ggcgtcgact tcgagcggta ggccgttgcg gccctggaag acgtgctcgg
     6181 ccggcacccc gaccgcctgg gccagctccg agtgcaggcg caggcgcttg tggtcgccgt
     6241 ggaccggcat cacaaactga ggtttggtca ggttcagcat cagcttcagc tcctcctgcc
     6301 agccgtggcc ggaggcgtgg atcggcgcgt ctttagcggt gatcacggtg gcgccgatct
     6361 cgtagatacg gtcgatcgtc tcgttcacgg cgcgctcgtt gccgggcacc ggggtggcgg
     6421 agaagacgac cgtgtcgccg gagtgcagct gcacgtcgcg gtggtcgttg ttggccatcc
     6481 ggcgcagtgc cgagagcggc tcgccctggc tgccggtcga gatcacgacg actttgtcgt
     6541 cggggaagtc ctcgatctcg cgcggctgga tgaagatgcc ctgcggcgcc tgggcgatgc
     6601 ccaggttcga agcgatgttg aagttcttgc gcatcgagcg cccgaccagg gcgaccttgc
     6661 ggtcgagctc gaccgcggca tcgatcacct gctgtacgcg gtggatgttg gaggcgaacg
     6721 aggtgacgat gatccggccc tcgcagcggg agaactcctg cagtagcgcc gggccgacgc
     6781 tcgactccga aggcgccacc ccaggccggt cggcgttggt cgagtcgccg cagagcaggg
     6841 tcagcccttc gcgaccgatc tcggccaggc gggagacgtc ggcgggcttg ccgtcgaccg
     6901 gggtctggtc gaacttgtaa tcgccggtca tcagcaccga gccggctttg gtcttgatca
     6961 ggaccccgcg catgtcagga atcgagtgcg cgaggtggac gagctcgagc tcgaacgggc
     7021 cgcgctggac tttctggccg ggcggcagct cctcgagcgg cgccgcctcg ccgagcttgt
     7081 gctcgtcgag cttcgagcgg accatgccga tcgtcagcag gccgccgtag atgaccggcg
     7141 gcgcgccgat ctcgcggagc acgtagggca atgccccgac gtggtcctcg tggccgtggg
     7201 tcaggacgat cgcctcgatg tcgtcggcgc gatcgcggag gtaggagaaa tcgggaagga
     7261 cgaggtcgat cccgtgcatc tcggcggtcg ggaacatcag gccggtgtcg acgacgacga
     7321 tcttgccgtc gtactcgatc acggtcatgt tcttgccgat ctcgccgagg ccgcccaagg
     7381 gcagcacgcg aagcttgtta ctcactgaat gttgctcact ctctgtaagg gaagtctgta
     7441 aaggaaaggg ctaccgggcg acgggaaggc cgtagccctc cagcgcagct ctcacagcag
     7501 cccgttgttc gctgtcggcg ggcaccatcg gcagccgcat ccgatccggg gcaagcccca
     7561 gcatctccaa cgcggccttc agaggtatcg ggttggtggt caccgaaagt ccctcgtaga
     7621 ccggcgtcag ctcggcgtcg atctgccgtg ctcgctccaa gtcgtttgcc tgtgcggcgt
     7681 cccacatttc gcgcatctgg tcgccgacga cgtgcgcggc gacgagaatg ccgccggcgc
     7741 cgccgaactc gagcacgcgc aggaaggtgt tgtcgttgcc ggcgaggacc ttgaggccgt
     7801 cgatcgggcc caggtcctcg tcgttggcct gcttgacggc gacgatgttg tcgacgtcac
     7861 ggccgagttc gccgagcagc tcgggatcgg cgttgacgat cacccgggcg gggatgttgt
     7921 acatcaccat cggcagctcg gggaccgctt cggcaacggc ttcgaagtgc gccctgatcc
     7981 cggcccggtt gggcttgttg tagtacggga cgacgaccag cgagccgtcg gcgccggcat
     8041 cggcggccat cttggtcagc tcgcgcgagt gctgggtgtc gttggtgccg gtgccgcaga
     8101 tcagcagcac ctcgtcgccg acctcagcgc gcacggcgcg gaagatctcg atcgtctcgc
     8161 cgtcggtcag ggtcgggcac tcgcccgtgg tgccggcgat caccagacca tgcgagccgt
     8221 gctcaagcag gtgggaagcg agccggcggg caccgtcgag atcgaccgtc ccatcctcac
     8281 ggaacggcgt cgccatcgcg gtgatcgcgc cttctataga agccattacg aagcagccta
     8341 gcgccacagc gcgacgactg gcgccatacg tccgcctaaa gcagccgctc gagcccgacg
     8401 gtgaagggct cgggcagctc gccgaccttg cgcacggcga gcagcacgcc gggcatgaac
     8461 gagcggcggt cgatcgagtc gtggcggatc gagagcgtct ggccctcgcc gccgaagatc
     8521 acttcctggt gggcgacgaa gccctgcagg cggaccgagt ggatcggctg gtggacgttg
     8581 ccgccggccg cgtcgatcag ctcggcggtc cgtttcgcgg tccccgaggg cgcgtcgagc
     8641 tttttgtcgt gatggagctc gatcacctcg cactcgggca tgtgcggggc gatcgtctgc
     8701 gcgaccatca tcatcagcac cgcgccgatc gcgaagttgg gggcgacgaa gcagttggcc
     8761 tccgacgcct cggccgccgc tttcgccgcg tcgaggtcga agccggtggt gccgacgacg
     8821 acgtggacgt cggcggccag acatgccttg acgttggcca gcgccgtgtc ggggatcgta
     8881 aagtcgacga ccacgtcgca gtcgccgagg atgtcctcga gcgaggtgcc gagcgtcgga
     8941 tccgccttgc cggccaactc cgtgtcggcg gccccttcga cggcttcgca gaccgcctgc
     9001 cccattttcc cggcggcgcc ggagaccccg accttgatcg ggaccgggct cacgctgcta
     9061 ccagggtctc gctgaccggc gccagggcgg cgcggaagcg gtcctcgtcg gcaccgacac
     9121 aggcggcgga gaggcggtcc tcggcgtaga gctcggcggc cagctcggtc aagtcgtcga
     9181 ccgcgatcgc gtcgacccgg gcgagcatct cgtcgaggct ttcgatcggc agcccgaaca
     9241 gggtggcgcg ggagatccgc gtcatccgtg ccgccgtcga ctccgaagag agcacgagcc
     9301 ggcccttgac gctctccttg gcccgggtca gctcctcggc cgagaccggc tcggagcgca
     9361 gccgtgcgag ctcggtgccg atgatcgagc aggcctcctc gacgttgtcc tcgcgggtgc
     9421 caacgtaggt cgcgaccagg ccctggtcgg tgaactgctc gttgtaggag ccgaccgagt
     9481 aggccagccc gcgcttctcg cgcacctcgc ggaacagccg cgaggaggtc gagccgccga
     9541 agatcgagtc gaggacggcg agcgcgtagc ggcgctcgtc gtcgcggcgc agccccggcg
     9601 ccccgaagca gatgtggtac tgctcggtct ccttctggtg gaagcagagt tgggcgccgc
     9661 cgtcgagctc catgtcgggc tgatcgccgc cggcgccggt cgggttgacc agcttctcgg
     9721 cgagggcgac gatccggtcg tggtcgaggt gcccggcggc accgacgacg atctcgcggc
     9781 cggcgtagcg ctcgcggtgg taggcgtcga tgtcggggat cgggatcgag gcggtcacct
     9841 cggcctcccc cagcacccgg cgcccgagcg gatgctggcc gaataccgct tcggcgagca
     9901 cgtcgtggac gcggtcctgc ggctcgtcct c
//
LOCUS       DASRIG010000203         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1404540, whole genome shotgun sequence.
ACCESSION   DASRIG010000203 DASRIG010000000
VERSION     DASRIG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404540"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFB52_05610"
     CDS             complement(<1..971)
                     /locus_tag="VFB52_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO05843.1"
                     /translation="MEQATLSHPPLAGTPEFPPGPRSPALVQALSYGRNPLGFLAVRQ
                     RRYGDLFTVRFPYYGRVVYVAQPELVKAVFTGDPAIFHAGEANATVLEPALGPNSVLT
                     LDDEPHMRQRKLLLPPFHGERVRRYGELIVEMTRREMRSWPTGEPFAIRPHTERITLA
                     VIMRAVFGVHDEARLARFERLIGEFAHRVDRITRFPFLRRSYGRFSPWDKFVRSREAL
                     DEFIYEEIGLRRAEPGHEERDDVLSLLLGARDEDGEPMNDQELRDEMVTVLGAGHETT
                     ATGLAWAVERLVRNPEVLAKLRTSLAAGEEDYLAATVKEVLRSRPVI"
     gene            complement(1025..1885)
                     /gene="purU"
                     /locus_tag="VFB52_05615"
     CDS             complement(1025..1885)
                     /gene="purU"
                     /locus_tag="VFB52_05615"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZO05844.1"
                     /translation="MMRAVTAPATSRLLLACPDRPGLIATVSGFLADAGLNIVDVDQH
                     STAEGRFFMRMVFDSVPASRRAELEQRFAEEIATPYEMDHRWAQSERRKKVAVMVSRE
                     DHCLSDLLWRWRNGELGGDLVTVISNHPDHASQVTAVGLPFHHVPVDKENRPAAEQRA
                     LELIGEVDLLVLARYMQILSDNFLTKVDAPAINIHHSFLPAFVGAEPYRRAHERGVKL
                     IGATAHYVTAELDAGPIIDQDVTRVSHSDEVPDLVRIGRDIERLVLARAVMAHLDDRV
                     LLDGERTVVF"
     gene            1983..2480
                     /locus_tag="VFB52_05620"
     CDS             1983..2480
                     /locus_tag="VFB52_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZO05845.1"
                     /translation="MLTKRSHIALLAILALVFPAVALASGGGSESKTGLSDAEVAPAP
                     ALEHSSHKFRQLVEDVADEIRTKEAERVKRKQREQREAFEDLPGGVSMETLEAIAECE
                     SGGDPTIVSSDGSYRGKYQFSFSTWETVGGSGDPAAAPEHEQDYRAALLYAQSGSSPW
                     PVCGL"
     gene            2530..>2904
                     /locus_tag="VFB52_05625"
     CDS             2530..>2904
                     /locus_tag="VFB52_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HZO05846.1"
                     /translation="MAEEKVPAPDPAGPTRKPPKEAVAEAKVRVPTRGNRKLESFLDA
                     VNSDDQVRTWWYMAQVNSERLGMSDHSWVHVQIVLNISLRLLRLLVKAGVEPNVVSDH
                     GLRDRDAEVVVAGGALLHDVGMS"
BASE COUNT          456 a          961 c         1042 g          445 t
ORIGIN      
        1 atgatcaccg ggcgcgagcg cagcacctct ttgaccgtcg ccgccaggta gtcctcctcg
       61 ccggcggcca gcgaggtgcg cagcttcgcc agcacctcgg ggttgcggac caggcgctcg
      121 accgcccagg cgagcccggt cgcggtcgtc tcgtgaccgg cgccgagcac cgtgaccatc
      181 tcgtcgcgaa gttcctggtc gttcatcggc tcgccgtcct cgtcgcgggc gccgagcagc
      241 agcgaaagca cgtcgtcgcg ctcctcgtga cccggttcgg cgcggcgcag cccgatctcc
      301 tcgtagatga actcgtcgag cgcctcgcgc gagcggacga atttgtccca ggggctgaag
      361 cgcccgtagc tgcgccgcag gaacgggaag cgggtgatcc ggtcgacccg gtgcgcgaac
      421 tcgccgatca gccgctcgaa gcgggccagc cgggcctcgt cgtgcacgcc gaagacggcg
      481 cgcatgatca ccgcgagggt gatgcgctcg gtgtggggac ggatcgcaaa cggctccccg
      541 gttggccagc tccgcatctc ccggcgggtc atctcgacga tcagctctcc gtagcggcgt
      601 acccgctcgc cgtggaacgg cggcagcaac agcttgcgct ggcgcatgtg gggctcgtcg
      661 tcgagggtga ggactgagtt cgggcccagc gcgggctcga gcacggtcgc gttcgcctcg
      721 ccggcgtgga agatcgcggg gtcgccggtg aagaccgcct tcaccagctc gggctgggct
      781 acgtagacga cccggccgta ataggggaag cggaccgtga agaggtctcc gtagcgtcgc
      841 tgccgcacgg cgaggaatcc gagcgggttc cggccgtagg agagggcctg gaccagcgcc
      901 gggctgcgcg gcccgggtgg gaactccggg gtacccgcga gtggcgggtg ggagagagtg
      961 gcttgctcca ttcacccaca gcctagccaa aactggccag tcagccagtt ttcctctcga
     1021 atggctagaa gacgacggtg cgctcgccgt cgagcagcac gcggtcgtcg aggtgcgcca
     1081 tcaccgcgcg ggcgagcacc aggcgctcga tgtcgcggcc gatccggacc agatcgggga
     1141 cctcatcgga gtggctgacc cgggtcacgt cctggtcgat gatcgggccg gcgtcgagct
     1201 cggcggtgac gtagtgggcg gtggcgccga tcagcttcac gccgcgctcg tgggcgcggc
     1261 ggtagggctc ggcgccgacg aaggcgggga ggaagctgtg gtggatgttg atcgccgggg
     1321 catccacctt ggtgaggaag ttgtcggaga ggatctgcat gtagcgggcg aggacgagga
     1381 ggtcgacctc gccgatcagc tccagcgcgc gctgctccgc cgccgggcgg ttctccttgt
     1441 cgacgggcac gtggtggaag ggcagcccga ccgcggtcac ctgggaggcg tggtcggggt
     1501 ggttggagat caccgtgacc aggtcgccgc cgagctcgcc gttgcgccag cgccagagca
     1561 ggtcggagag gcagtggtct tcgcgcgaga ccatcaccgc caccttcttg cgccgctccg
     1621 actgcgccca gcggtggtcc atctcatagg gcgtggcgat ctcctcggcg aaccgctgtt
     1681 ccagctccgc tcggcgcgag gccggcaccg agtcgaagac catgcgcatg aagaaccggc
     1741 cctccgcggt cgagtgctga tcgacgtcga cgatgtttag cccggcgtcg gcgaggaagc
     1801 cgctgacggt cgcgatcagc cccggccggt cggggcaggc gagcagcagg cgcgaggttg
     1861 ccggtgcggt cacggctcgc atcatgcctg ctcagccctc agttcgggct cattcgccca
     1921 aaaagaaatt gtgtgatcgc cgacacacac agctcggggt tttccctgcg accgttcaac
     1981 gcttgctcac gaagcgctca catattgccc tgctcgcaat cctcgccttg gtcttcccgg
     2041 ccgtcgccct cgccagtggt ggcggctcgg aatcgaagac cgggctctcc gacgccgagg
     2101 tcgctcccgc tccggcgctt gagcactcca gccataagtt ccggcaactg gtcgaggacg
     2161 tcgccgacga aatccgcacg aaggaagcgg aacgggtcaa gcgcaagcag cgcgaacagc
     2221 gggaagcctt cgaagacctg cccggcggcg tctcgatgga gaccctggaa gcgatcgccg
     2281 agtgcgagtc cggcggcgat ccgacgatcg tctcctccga cggctcctac cgcggcaaat
     2341 accagttctc gttctcgacc tgggaaacgg ttggcggcag cggcgatccg gccgcggcgc
     2401 ccgagcacga gcaggactac cgcgcggcgc tgctttacgc gcagtcgggc tcgagtccct
     2461 ggccggtctg cggtctctag tcgcggaatc gacacagagg gggccggtgg cggccctacg
     2521 atccccggga tggccgagga gaaagtccca gcccccgatc ccgccgggcc gacgcgcaaa
     2581 ccgccgaaag aggcggtcgc cgaggcgaaa gtgcgggtgc cgacccgcgg caaccgcaaa
     2641 ctcgagagct tcctcgatgc ggtcaactcc gacgaccagg tgcggacctg gtggtacatg
     2701 gcgcaggtca actcggagcg gctggggatg tcggaccact cctgggtcca cgtccagatc
     2761 gtgctcaaca tctcgctgcg gctgctgcgc ctgctggtca aagcgggggt cgagccgaac
     2821 gtggtcagcg accacgggtt gcgcgaccgc gacgccgagg tggtggtcgc cggcggcgcc
     2881 ctgctgcacg acgtcggcat gtcg
//
LOCUS       DASRIG010000204         4155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_621720, whole genome shotgun sequence.
ACCESSION   DASRIG010000204 DASRIG010000000
VERSION     DASRIG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621720"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFB52_05630"
     CDS             complement(<1..248)
                     /locus_tag="VFB52_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05847.1"
                     /translation="MPILFASVTDFFLDPIVNAGTDFISTTGLPAVFVLMALESACLP
                     VPSEAIMLFAGSSVAAGELTLFGVVAAGVLGNLVGSWVA"
     gene            314..2191
                     /locus_tag="VFB52_05635"
     CDS             314..2191
                     /locus_tag="VFB52_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZO05848.1"
                     /translation="MNHPVYRNRVLQVLADGVLVALAYYLAFQLRFLDEGGWPQRYQE
                     LFAQSVGFVVAGKLIIFAVSGMYQKWWRYVSGRDFMLILRAVAISSAILVVAFVVLKP
                     FSPALPRSVVVTDFFLTLFLIAGARLAVRLIVERPSRADRLPKHEVLVIGAGSGGQMV
                     VRELQLNPNLGATAIGFLDDDPRKRGMRMLGLKVLGSTDEIDAILDEAMPDEVVIAIP
                     SAPGTLRARVVAACREREIRVRTLPTVFELLRGGVQLNRQLREVQVEDVLGREPIVRE
                     LDRVGAYLRDRIVLVTGAGGSIGSELCRQIAQVKPKLLVLLDHAEDNLFEIEREMIEV
                     RHFTNVEAVLADCKEAHRMLEVMQRFKPEIVFHAAAYKHVPLMEANPLEAVRNNAIAT
                     RITAETAAASNVERFVLISTDKAVNPATVMGASKAMAEWIVEQAGENHPGTRFASVRF
                     GNVLASSGSVVPIFRSQIARGGPVTVTHPEMTRFFMTIPEAVQMVIRAGDIGAGKGEV
                     FVLDMGEAVKIVDLAHNMIRLAGYEPETDIAIEFTGPRPGEKLHEELYDSSEDPQPTA
                     ARRIYRAVRSTPLDSEWVESTLEALEGLVLAGDEANLAERVVDLIAARGGDSAGVALD
                     E"
     gene            2207..2989
                     /locus_tag="VFB52_05640"
     CDS             2207..2989
                     /locus_tag="VFB52_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZO05849.1"
                     /translation="MADAIEQIGSYAGLAAFLGLAVLSLLYFTQARDLRRLRDWAGSA
                     PERDAERKEATSTIAAQRAEELKALEEARTAEHAAISQREQRRKRREEGLPEETRGER
                     LRGFFSRLGERLAEPRWLVVIFIVLVAIVGGVAYTVLSGSDDDAKGKQGKQAAAASKQ
                     VKPSEVEVAVLNGTATPGLAATFGDKIEGKGYQLGEVTNSDEPAETSVVMFTTGHKPE
                     AKKVANQLDISEVRMMTTDIKEDSGEASVAVIIGEDDAAETG"
     gene            2970..3482
                     /locus_tag="VFB52_05645"
     CDS             2970..3482
                     /locus_tag="VFB52_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05850.1"
                     /translation="MPRKPASRYAAATVFALLVLATVAAFAWSQKQKREPLVLDKVTI
                     GIDGKNSFTPNGDCRFDHIRIRFRTTISDDGTVQIIRPSGAKMITLARDEFLKRYRFH
                     TFFWDGRRAGGGIAPPGRYKLRVKLLGEDRVLVPAGTIKLHKGTTDKGSACDAGGPGG
                     VAPNAAEPAP"
     gene            3479..>4155
                     /locus_tag="VFB52_05650"
     CDS             3479..>4155
                     /locus_tag="VFB52_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05851.1"
                     /translation="MSEVLAGAGVLLAAGAGAAAILLPPGRLRALAMLAALILFPVLI
                     LGDQWHTSQITDLRHDGGRIVALLVAGTAVVAALTFVFRRWPILIPLAIVFALPFRVP
                     LEAGGDTANLLVPLYLVVAGGVLATLWRDWNRSEQPTPASGPTLWLPRILAAVLALYA
                     LQVLYSEDFSKGLQNVCFFYVPFAVVYALISDVKWDRRTLTLVLAVVGVEAAIFVGIG
                     TVEYLSR"
BASE COUNT          667 a         1453 c         1398 g          637 t
ORIGIN      
        1 gcgacccagg agccgaccag gttgccgagc acgcccgccg cgaccacccc gaacagggtc
       61 agttccccgg cggcgacgct ggagccggcg aacagcatga tcgcctcgct cgggaccggc
      121 aagcaggcgg actcgagcgc catcagcacg aacacggcgg gcaaacccgt ggtgctgatg
      181 aagtcggtcc ccgcgttgac gatcgggtcg aggaaaaagt cggtaacgct ggcgaagagg
      241 atcggcatca aatgaagcgg ggattgtatg gatgtggctt tacagcgact aaactcgccg
      301 gttcatgggc cggttgaacc accctgtcta tcgcaaccgg gttctgcagg ttctggcgga
      361 cggcgtgctc gtcgccctcg cctactacct cgcgttccag ctgcgcttcc tcgatgaggg
      421 cggctggccc cagcgctatc aagagctctt cgctcaatcc gtcggcttcg tcgtcgccgg
      481 caagctgatc atcttcgccg tctccgggat gtaccagaag tggtggcgct acgtgagcgg
      541 ccgcgatttc atgctgatcc tgcgcgcggt cgcgatctcg agcgcgatcc tggtcgtcgc
      601 cttcgtcgtc ctgaagccgt tctcgccggc gctgccgcgc tcggtcgtgg tcaccgactt
      661 cttcctcacc ctgttcctga tcgccggcgc ccgcctcgcc gtgcgcctga tcgtcgagcg
      721 cccctcgcgc gccgaccgcc tgcccaagca cgaggtgctg gtgatcggcg ccggctccgg
      781 cgggcagatg gtggtccgcg agctgcagct caacccgaac ctcggcgcga ccgcgatcgg
      841 cttcctcgac gacgacccgc gcaagcgcgg catgcggatg ctggggctga aggtgctcgg
      901 ctcgaccgac gagatcgacg cgatcctcga cgaggcgatg cccgacgagg tggtgatcgc
      961 gatcccctcg gcgccgggca ccctgcgcgc ccgggtggtc gccgcctgcc gcgagcgcga
     1021 gatccgcgtg cgcaccctgc cgaccgtgtt cgagctgctg cgcggcggcg tgcagctgaa
     1081 ccgccagctg cgcgaggtcc aggtcgagga cgtgctcggg cgcgagccga tcgtgcggga
     1141 gctcgaccgc gtcggcgcct acctgcgcga ccgcatcgtg ctcgtcaccg gggccggcgg
     1201 ctcgatcggc tcggagctct gtcgccagat cgcccaggtc aaaccgaagc tgctggtcct
     1261 gctcgaccac gccgaggaca acctcttcga gatcgagcgc gagatgatcg aggtgcgcca
     1321 cttcaccaac gtcgaggcgg tgctcgccga ctgcaaggag gcgcaccgga tgctcgaggt
     1381 gatgcagcgc ttcaagcccg agatcgtctt ccacgccgcc gcctacaagc acgtgccgct
     1441 gatggaggcc aacccgctgg aggcggttcg caacaacgcg atcgcgaccc ggatcacggc
     1501 cgagaccgcg gccgcctcga acgtcgagcg cttcgtcctg atctcgaccg acaaggcggt
     1561 caaccccgcg accgtgatgg gcgcctcgaa ggcgatggcc gagtggatcg tcgagcaggc
     1621 cggcgagaac caccccggca cccgcttcgc ctcggtccgc ttcggcaacg tgctcgcctc
     1681 ctcgggcagc gtggtgccga tcttccgcag ccagatcgcc cgcggcggcc cggtcacggt
     1741 cacccacccg gagatgaccc gcttcttcat gacgatcccg gaggcggtgc agatggtgat
     1801 ccgggccggc gacatcggcg ccggcaaagg cgaggtgttc gtgctcgaca tgggtgaagc
     1861 ggtgaagatc gtcgacctcg cccacaacat gatccggctg gccggctacg agcccgaaac
     1921 cgacatcgcg atcgagttca ccggcccgcg cccgggcgag aagctgcacg aggagctcta
     1981 cgacagctcc gaggacccgc aaccgacggc ggcgcggcgg atctaccggg ccgtgcgctc
     2041 gacaccgctc gactccgagt gggtagagtc gaccctggag gcgctggaag gattggtgct
     2101 ggccggagac gaggctaacc tggccgagcg agtggtggat ctgatcgccg ccaggggtgg
     2161 cgactccgct ggtgtcgcgc ttgacgagta gcctctcgca gccactttgg ccgacgcgat
     2221 tgaacaaatt ggctcctacg ccgggctggc ggccttcctc ggattggccg ttctctccct
     2281 gctttatttc acccaggcgc gtgacctgcg gcgcctgcgc gactgggccg ggtcggcgcc
     2341 cgagcgcgac gccgagcgca aggaggcgac ctcgacgatc gccgcccagc gcgccgaaga
     2401 gctgaaggcg ctcgaagaag cgcgcacggc tgagcacgcg gcgatcagcc agcgcgagca
     2461 gcggcgcaaa cggcgcgagg aggggttgcc cgaggaaacc cgcggcgaac gcttgcgcgg
     2521 ctttttctcg cgcctcggcg aacggctggc cgaaccgcgc tggctggtcg tgatcttcat
     2581 cgtcctggtg gcgatcgtcg gcggcgtcgc ctacaccgtg ctaagcggct ccgacgacga
     2641 cgccaagggc aaacagggca aacaggccgc cgcggcgagc aagcaggtga agccgagcga
     2701 agtcgaagtc gcggtcctca acggcaccgc gacgcccggc ctggcggcga ccttcggcga
     2761 caaaatcgag ggcaagggct accagctggg cgaagtcacc aactccgacg aaccggccga
     2821 aaccagcgtc gtgatgttca ccaccggcca caaaccggag gcgaagaagg tcgccaacca
     2881 gctcgacatc agcgaagtcc ggatgatgac gaccgacatc aaagaagact cgggggaagc
     2941 cagcgtcgcg gtgatcatcg gcgaggacga tgccgcggaa accggctagc cgctacgcgg
     3001 cagccaccgt cttcgcgctg ctggtcctcg caaccgtcgc cgccttcgcc tggtcccaga
     3061 agcagaagcg cgagccgctc gtgctcgaca aggtgacgat cgggatcgac ggcaagaaca
     3121 gcttcacgcc gaacggcgac tgccgcttcg accacatccg gatccgcttc cgcaccacga
     3181 tctccgacga cggcaccgtc cagatcatcc gcccgagcgg cgcgaagatg atcaccctcg
     3241 cccgcgacga gttcctcaag cgctatcggt tccacacctt cttctgggac ggccggcgag
     3301 ccggcggcgg gatcgcgccg cccgggcgct acaagctgcg cgtgaagctg ctcggtgaag
     3361 accgggtgct ggtcccggcg ggcacgatca agctgcacaa ggggaccacg gacaaaggca
     3421 gcgcctgcga cgccggcggc cccggcggcg tcgccccgaa cgccgcggag ccggcgcctt
     3481 gagcgaggtc ctcgccgggg ccggcgtcct gctcgcggcg ggcgccggcg ctgcggcgat
     3541 cctgctgccg cctgggcgtc tgcgggcgct ggcgatgctc gcggcgctga tcctcttccc
     3601 ggtcctgatc ctcggcgacc agtggcacac ctcccagatc accgatctgc gccacgacgg
     3661 cggccggatc gtcgccctgc tggtggccgg cacggcggtc gtcgccgccc tcaccttcgt
     3721 cttccgccgc tggccgatcc tgatcccgct cgcgatcgtc ttcgcgctcc cgttccgcgt
     3781 gcccttggag gccggtggag acaccgcgaa cctcctcgtg ccgctttacc tggtcgtcgc
     3841 cggaggagtg ctcgcaaccc tgtggaggga ctggaaccgc agtgagcagc ccacaccggc
     3901 cagcgggccg acgctttggc tgccgcgcat ccttgccgct gtcctcgccc tctacgcgct
     3961 tcaggtcctc tactcggagg acttctcgaa agggctgcag aacgtctgct tcttctacgt
     4021 gcctttcgcc gtggtctacg ccctgatcag cgacgtcaag tgggatcggc ggacgctgac
     4081 cctggttcta gcggtggtcg gcgtcgaggc ggcgatcttc gtcgggatcg gcaccgtcga
     4141 gtacctcagc cggga
//
LOCUS       DASRIG010000205         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3765178, whole genome shotgun sequence.
ACCESSION   DASRIG010000205 DASRIG010000000
VERSION     DASRIG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3765178"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFB52_05655"
     CDS             <1..507
                     /locus_tag="VFB52_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZO05852.1"
                     /translation="EDDCGCAYVTEHDRELGQRSVVWTLEHTDQRSKDYMRELPFDLR
                     FELGAKRVRLVHGSPRKVNEYLFEDKPAKTFERIAAGADCDILVFGHTHKPWIAEYGG
                     VQFVNCGSVGKPKDGDRRGAFAVLEAADGGVEARIERFAYDAELASREVAAAGLPAEF
                     AEKLLVAA"
     gene            520..1137
                     /locus_tag="VFB52_05660"
     CDS             520..1137
                     /locus_tag="VFB52_05660"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZO05853.1"
                     /translation="MKHVLFVCNHNAGRSQMAQAFFERDAPADMRAESAGSEPAARLW
                     PGVVEAMAEVGIDIAGRKPRKLLREMQLHADWAVTMGCGDACPYVPTTVESWDVPDPA
                     GLPPEGVRRIRDEVEARVQDLLANRIEEIRTDPTAHRLRLAAMLPGLIEEFEGRRPDA
                     VIRACADAVLSRYDDVPIRGHVQAIAHRRTRECLAADTCQELIAG"
     gene            1134..1847
                     /locus_tag="VFB52_05665"
     CDS             1134..1847
                     /locus_tag="VFB52_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZO05854.1"
                     /translation="MIPLPRRLFAEFLGSAFLAATVIGSGIAAAELSPGDTGLQLLEN
                     AAATATGLFTFILMFGPISGAHFNPVVSLVDAAFGGLRWRHAFAYIPAQILGCITGAV
                     AANVMFDLSAISISTTHRASPPHLFAEVIATLGLLLIIFALARSRRGSLAPAAVGAYI
                     GAAYFFTSSTSFANPAIDIGRMFSDTFAGIAPASVPSYIAVQLVGGVVAFFVIRILYP
                     DLTPDEAAEVMQPHQEGLA"
     gene            1947..2828
                     /locus_tag="VFB52_05670"
     CDS             1947..2828
                     /locus_tag="VFB52_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05855.1"
                     /translation="MPPSKRLYRSAGSRHRLVFVALLAVGAAAIPTITSAAAPSGSSD
                     LRITKSDSPDPVRVGSQLTYTIGVENRGPDPATGVIVTDNLPKTVNLVSASGPGGTCV
                     QQGQNLRCPIGALAPVGVNYGGTQTAVTIVVSPRSSGTIRNTATVKGDQKDPATGDNK
                     ATATTRVLGPARCRGLDANVAGTSGDDVLSGTPGPDVIAAGGGRDRIVSLAGQDLICA
                     GSGADYVASGSAADRVFSGAGADRLLGRGGPDLLRGSAGNDVLKGGRGSDRLRGGQDF
                     DRCSGGPGADSERGCER"
BASE COUNT          455 a         1109 c          977 g          428 t
ORIGIN      
        1 gaagacgact gcggttgcgc ctacgtgacc gagcacgacc gcgagctcgg ccagcgctcg
       61 gtcgtctgga ccctcgagca caccgaccag cgctccaagg actacatgcg cgagctgccc
      121 ttcgacctgc gcttcgagct gggcgccaaa cgcgttcgcc tcgtccacgg ctcgccacgc
      181 aaggtcaacg agtacctgtt cgaggacaag ccagcgaaga cgttcgagcg gatcgccgcg
      241 ggcgccgact gcgacatcct cgtcttcggc cacacccaca agccctggat cgccgagtac
      301 ggcggggtgc agttcgtcaa ctgcggctcg gtcggcaagc ccaaggacgg cgaccggcgc
      361 ggcgctttcg cggtgctgga ggccgccgat gggggagtcg aggcgaggat cgagcgcttc
      421 gcttacgacg ccgagctggc ctcacgcgaa gtggcggcag cggggctgcc cgccgagttc
      481 gccgagaagc tgctggtcgc tgcctagaaa ggggaggcaa tgaaacacgt tctgttcgtc
      541 tgcaaccaca acgccgggcg ctcgcagatg gcgcaagcgt tcttcgagcg cgatgcgccc
      601 gccgacatgc gcgccgagtc ggcaggatcc gaaccggccg cgcgtctgtg gcccggagtt
      661 gtcgaggcga tggccgaggt gggaatcgac atcgccgggc gcaagccgcg gaagctgctg
      721 cgggagatgc agctacacgc cgattgggcg gtgacgatgg gctgcggcga cgcctgcccc
      781 tacgtcccca ccacggtcga gagctgggac gtacccgacc cggccgggct cccgccggag
      841 ggagtgcggc ggatccgcga cgaggtcgag gctcgggtgc aggatctgct cgcaaaccgg
      901 atcgaggaga tccgcaccga cccgaccgcg caccggctgc gcctggcggc gatgctgccg
      961 gggctgatcg aggagttcga aggccggcgc cccgacgccg taatccgcgc ctgcgccgac
     1021 gcggtcctct cccgctacga cgatgtcccg atccgcggcc acgttcaggc catcgcccac
     1081 cgacgcacgc gcgagtgcct ggccgccgac acctgccaag agctgatcgc cggatgatcc
     1141 ccctaccccg ccgcctcttc gccgagttcc tcggctcggc tttcctcgcc gcgaccgtga
     1201 tcggatcggg tatcgccgcc gccgaactct cgccgggcga caccggcctg cagctcctcg
     1261 agaacgccgc cgccacggcg acgggcctct tcaccttcat cctcatgttc ggcccgatct
     1321 ccggcgccca cttcaatccg gtcgtctccc tggtcgacgc cgccttcggc ggcctgcgct
     1381 ggcgccacgc cttcgcctac atccccgccc agatcctcgg atgcatcacc ggggccgtgg
     1441 cggcaaacgt gatgttcgac ctctcggcga tctcgatctc gaccacgcac cgcgcctctc
     1501 ccccgcacct gttcgccgag gtgatcgcga ccctcggcct gctcctgatc atcttcgccc
     1561 tggcccgcag caggcgcggc agcctggcgc cggcagcggt cggcgcctac atcggcgccg
     1621 cctacttctt caccagctcg accagcttcg ccaaccccgc gatcgacatc gggcggatgt
     1681 tctccgacac cttcgccggc atcgccccgg cctcggtgcc ctcctacatc gccgtgcagc
     1741 tcgtcggcgg cgtcgtcgcc ttcttcgtga tccggatcct ctatccggat ctgacgccgg
     1801 acgaggctgc ggaggtcatg cagccgcacc aggaaggttt ggcctgacgg agagcctccc
     1861 aacacgttga agcagggaag agacgcaagc gctcgcgtgg ccgccccacc gacggcggcc
     1921 tcagcaacgg gaagagaggg actccgatgc caccgagcaa acgcctttac cgatccgccg
     1981 ggtcgcgcca cagactcgtt ttcgtcgcgc tcctggccgt tggcgccgcc gcgattccga
     2041 cgatcacctc agccgcggct ccgtcgggct cgtccgacct gcggatcacc aagagcgaca
     2101 gcccggaccc ggtgcgcgtc ggctcgcagc tgacctacac gatcggggtc gaaaaccgcg
     2161 gcccggaccc ggcgaccggc gtgatcgtca ccgacaacct tccgaaaacg gtcaatctgg
     2221 tctcggcctc aggtccgggc ggaacctgcg tccagcaggg ccagaatctc cgctgcccga
     2281 ttggcgccct ggctccggtc ggcgtcaatt acggcggcac gcagaccgcc gtgacgatcg
     2341 tcgtctcgcc gcgcagctcc gggacgatcc gcaacacggc cacggtcaag ggcgaccaga
     2401 aagaccccgc caccggagac aacaaagcga ccgcgacgac gcgcgtgctc gggcccgccc
     2461 gctgccgtgg gctcgacgcc aacgtcgccg ggaccagcgg tgacgacgtc ctctccggca
     2521 cccccggtcc cgacgtgatc gcagccggag gcggccggga ccggatcgtc tcgcttgccg
     2581 ggcaggacct gatctgcgcc ggcagcggcg ccgactacgt cgcctcaggc tccgccgcag
     2641 accgcgtctt cagcggcgcc ggagctgacc gactgctcgg ccgcggcggc cctgacctgc
     2701 tgcgcggctc cgccgggaac gacgtcctca agggcgggcg cggctccgac cggctccgcg
     2761 gtggccaaga cttcgatcgc tgctcgggcg gccccggtgc ggactccgag cggggctgcg
     2821 agcgctgaag ccggcggcgc ccgggcccag cgggcgttga cgcgggctcc tggtttcctc
     2881 aagatgcgct ctcgatgggc gcgccgacga tcgagaccga agggctgacc aagctcttcg
     2941 gcaaccatcg cggcatcgag gacgtcagc
//
LOCUS       DASRIG010000206        12484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2727462, whole genome shotgun sequence.
ACCESSION   DASRIG010000206 DASRIG010000000
VERSION     DASRIG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727462"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFB52_05675"
     CDS             complement(<1..403)
                     /locus_tag="VFB52_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05856.1"
                     /translation="MLSAPRLALAAIVCLALLAAGAGSALGAVRYAAPGASGAEPCNP
                     GPCSLVNAVNAAKDGDRVVVAPGQYLVADDLEVDSAIDVGGEPGAARPLIGLVQKTLY
                     VEHPAAVVHDLSLFLTEEAMAYTLMMESGTVE"
     gene            434..880
                     /locus_tag="VFB52_05680"
     CDS             434..880
                     /locus_tag="VFB52_05680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO05857.1"
                     /translation="MGKIGGESSVAIDAPIEAVYAVAADAEGAPRWQPEIEVAECLER
                     DGAGDQLRVRIETETPIKRLTSVLVYSYESPTRISWRQEDGDLKSVEGSWELRDLGDE
                     RTEATYKLEVDPGRILGMALRGPVVGILRGKLVDSMPGKLKAFLER"
     gene            932..2185
                     /locus_tag="VFB52_05685"
     CDS             932..2185
                     /locus_tag="VFB52_05685"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO05858.1"
                     /translation="MSTVTAGSLEEWQDRVRRQTAVELRPNLDALVGECVDYVQDSLP
                     QLDVDEELADGLYESARDNLDRIFGMLAQGIPVSDTVAPPGAIRYAEIMVRRNTPLAT
                     LLRAYRLGTMWFWTLWRREIVKRIDEPELLMQAVDHSMSFVFDYVDLVSEQVTEEYAL
                     QRSRWARSAAALRHETVEGLLEGSLTDVDVAGSRLGYDLRPTHLALVLSASPKSDTED
                     VVARLEHCAQELAARVTPRRPLLVPDGRTTMWAWLSFEHAPGGDVLAEVEQDRKLLAE
                     VCVTCGTPQAGLAGFVASHQEALHTRELMRLSNRGRGRLVRYDEVALVSLLAADLDRA
                     RNFVARELGELGNSDDATTRIRATVQVLLEESGRTAVAARRLGVHQNTVSYRVRQAEE
                     ILGHSLKQRRFEVETALRLRDLLVE"
     gene            2285..4114
                     /locus_tag="VFB52_05690"
     CDS             2285..4114
                     /locus_tag="VFB52_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO05859.1"
                     /translation="MQAITIDIDTGGTFTDAFVVRDGHPHRVKVLTTPHDFALCFSQV
                     IERAAEVLDLDTAELLRETACIRYATTVGTNTVIERTGPRIGLIVDPESAAGGVPEGA
                     FVEPEMVTAVAPGDGDGAVVEVAKELMARSARGLVCAMPSDADGAEAERAVLRGFHQH
                     YPRHCLDAVPLTLSHEIAPDPDDARRTATALFNAYVHEDVAHYLYRAEDWLRDHGYTR
                     PLLVVHNDGGCARVAKTVAGRTYNSGPTAGLLGAEAIAALCGIDHLLTFDVGGTSLDV
                     GFLGEGRAHFKEHGRVTGVEVSFSMPDIQVLGAGGGSIAHVDDGVLAVGPQSAGANPG
                     PACFGLGGSEATVTDADVVLGIIDAKDFLGGRMELHADAATAAVGRIAERLGISTVEA
                     AAQIRSTLHDRMAERIGAELTERGLDPARTTMLAYGGGGPTHAAPVAARIGIGTVVTL
                     PFGAVFSAFGASSADVRHVYFAVPGSDAEELLRRTALLDMGGEGFAPEQVEIELDRVH
                     RHGAERLRLTAVAKLDHPALEAGAAEGHEPEPSGSREIHWPGEGTVPTPVHDAAELRP
                     GAVVSGPAVLEAEDTTYVVPRGWTHRVDGHGIGWMTRTAKEES"
     gene            4114..4449
                     /locus_tag="VFB52_05695"
     CDS             4114..4449
                     /locus_tag="VFB52_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HZO05860.1"
                     /translation="MTAPTGAREAITEYLEIDLETETWRCRRCDHEIGSAREDYKRGL
                     LLYDRDPREIHPAKIDAPYSYAPDPEWCRMVECYCPGCGTQIEVEYLPPGHPITHDIE
                     IDVDALKVR"
     gene            4449..6581
                     /locus_tag="VFB52_05700"
     CDS             4449..6581
                     /locus_tag="VFB52_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO05861.1"
                     /translation="MEINVDIGGTFTDCFASDGDKVASAKALTTHHDLSHGFMTAVED
                     LAASFALAPDELLGSTEAIRYATTLGTNALIERTGPRLGLLTTTGFEDVVPIGRCRAY
                     GEGLSHVDNRNLARAQRPEPLIPRELIAGLRERVDQAGQVVMPLDPDEVREKVRELVD
                     RGVRGFVVSFLWSFKNPEHELLVREIIEEEYPSSYLGRFPVILAHEVSSRSGEYVRSM
                     TSILDAYLHRFTADRMSDLRFKLRERGYGGPLLLVHNSGGMGGLARTTPLQTIHAGPV
                     AGLYGSRHMASRFELGKVVTTDMGGTSFDIGVVVEGSVRFYEFNPVIDRWLVQMPMMD
                     IKAVGAGGGSIASYDGFRGVAVGPRSAGSTPGPACYGQGGTEPTVTDANLVLGYLNPD
                     YYHGGSMALDTRRAERAIERRVARPMEASVAEAARSIRAVVDNNMGDAIFKEIVLKGY
                     DPRDFTVFSFGGGGPLHACSYSEVLQIPRVVVPPHSPVFSASGAAGLDLLHIYERSEH
                     IVLLNPLFKTVLEDYGQINDVVDQFMAEAREDFADEGHGEDAIEYALELDVRSGGQLY
                     LTTIKSPTLKIESREDLAAVLEAYFTEYADRFGEFAVTREVGASVETVRLRAWVKRQR
                     WEPELLPAPGHPVEETRKGERDCWWERAGGFEPTPIYDYDRLGPGHEVAGPAVLEGRD
                     TTVVVNPGWSAAMDDHGFLTLTNEEERA"
     gene            6578..8719
                     /locus_tag="VFB52_05705"
     CDS             6578..8719
                     /locus_tag="VFB52_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO05862.1"
                     /translation="MKPPETLTPAAPARPEDAEAFFAELLNDKPVMYGPSPGIIDDNQ
                     LSPRSADEEEALLVATDEVELTIAQNRLEVILESAKEMLEQTGAAPGAKWGDLTCGLY
                     SAAGDLALASSSGVVVFAACASAPVKHIVKDWIEEPTVGVRPGDVFYHNDARYGGVHN
                     ADQSLFIPIFAGERLIAWAGATIHEGENGATEPGGLSPSALSVYDEGLKISPIKVAEN
                     YVFRRDIVNLMQNNVRDPLMQLIDMRAKLAAAMRIEERVMEVVADRSAEVLLATLRNA
                     LERTRAEVKRRFAEFPDGVFRAVAFCDTTLLEDRMLKLELRLEKRGDKVVCNTHGSGP
                     AIPDRALNMDPTFARSLFSNNLMNFFWSDLPRNAGYVSAIEFEFEEGSIAKSGAECPN
                     ALSMVAACFYQTLMHVCMSKLAFNRPGDVQIIAPWYGMTQAMFYGGLNQWGMPVANLM
                     IDINSMGGGAHPDRDGEHSCAPFFASMADYGEIEDRETELPILALGRSLTVDNHGFGR
                     HRGGSSVECMYMVWGVPEFAFGSLATGSKFPTIPGLFGGYGGPSSPLTAFKAESPEAV
                     KAAMREAPHAVPTTADELHAHGGLPGEAETRRATQTANFLGEGDLWILQVGGGGGYGD
                     PLERDPELVMADLRQKRISGPVAENVYRVVYDAERLVVDAEATAGARDAERRARLTRG
                     LPYDDFVRSWRREEPPPEIPFLGRWDWEAQR"
     gene            8716..9495
                     /locus_tag="VFB52_05710"
     CDS             8716..9495
                     /locus_tag="VFB52_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZO05863.1"
                     /translation="MSAVGEAPPRHDPAARHGVAIGEGAAALDLMSTMRAMRKLRPDP
                     VPRETLERLVEAATWAPSGGNTQEYSFTIVDDRETMAKVAKIWRRTCAYYTAAQRPLP
                     PPYVKPEAWDKVMDSLEVLADDFEETPALIVACYDMSGILKRMIKGWKHTLRGARAIG
                     VWQTLRFLPRIPRFMSTGEAASIYPGVQNMLIAARALGLGATLTTWHTLYEGDYRRAL
                     GLSRRTKIYAIVPVGYPRGHFGPVSRRPAAEAIRYTGEATG"
     gene            9499..11064
                     /locus_tag="VFB52_05715"
     CDS             9499..11064
                     /locus_tag="VFB52_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05864.1"
                     /translation="MLLIDTFDRGVARNPEGTCFLTADESVRMSYTEVAALSHRVAAA
                     LAAHGVEAETRVGVLSPNAPIGFCCVLGALRLNASWVPLNAKATAADLVPLLELTDCE
                     VLFYHPALLPLAEAVLEGCDTVRLAIAIEEGGRPGDPRLGEWMAPEGTTVPQPPHEPD
                     RTAIMFGTGGTTGRSKAVPITHRMAEAMNLAFAGHMPEPQPPTMAMAAPMTHAAGPIC
                     FPVFAEGGTVVVHDGVVPDLLLGSIERHRVSRLFLPPTAIYALLSVPDVRSYDFSSLR
                     HFIYAAAPMAVDKLEEALDVFGPVMTQTFGQAEAPMICTVMTPAEHVAAAADPALRNR
                     LASAGRSSLVAEVAIMDDDGGLLGPGEVGEIVVRGSLVMAGYHKDPEQSQATRRPGGW
                     HGTSDVGRIDEHGYVFIVDRKRDMIITGGFNVWPSEIEQVIHTVEGVRDCAVIGLPDE
                     KWGEAVTAVVELREDAPGIEAEAVTALCREQLGPVKTPKQVIFRELPRSPNGKVLKRA
                     LRDEYWTDRARAV"
     gene            complement(11061..12242)
                     /locus_tag="VFB52_05720"
     CDS             complement(11061..12242)
                     /locus_tag="VFB52_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZO05865.1"
                     /translation="MNKAFVVGVGMTKFEKPGRGERDYPEMAREAGSRALADAGLAYG
                     EVEQACVGYVYGDSTAGQTAVYTLGTTGIPVVNVNNNCSTGSTALFYARQLVAGGLAE
                     CVLALGFEKMARGSLPESAFPDRVSPFDRFVERSAELSPGCGGPMALELFGGAGREHM
                     RRYGTTEEQFARIAVKNHLHSGNNPYAQFRDPCTLEEVLGSTSYGEPLTKLQCSPTSD
                     GAAAALIASERFVAERGLEARAVEIVAQAMVTDLPSTFAGDEIALVGAEMSRAAADSV
                     YDRTGLGPEDVDVIELHDCFSVNELITYEALGLCGEGEGGDLIDDGATTYGGNWVVNP
                     SGGLISKGHPLGATGLAQCSELTWQLRGEAEARQVAGAKVALQHNIGLGGAAVVTMYA
                     R"
BASE COUNT         1844 a         4567 c         4378 g         1695 t
ORIGIN      
        1 tttccacggt tccggactcc atcatcagcg tgtaggccat cgcctcttcg gtcaggaaca
       61 gcgagaggtc gtggacgacc gctgccgggt gttcgacgta gagcgttttt tgcaccaggc
      121 cgatcagcgg tcgcgccgcc ccgggttcac cgccgacgtc gatcgccgag tcgacttcga
      181 ggtcgtcggc gaccaggtac tgaccgggcg cgacgaccac ccggtcgccg tctttggcgg
      241 cattgaccgc gttgaccagc gagcaggggc cgggattgca gggctccgcc ccgctcgccc
      301 ccggcgccgc gtagcgcacg gccccgagcg ccgacccggc gccggccgcg agcagggcaa
      361 ggcagacgat ggccgcgagg gccaggcggg gggcggacaa cacgcggcga tcctagaggt
      421 agcctgcgga catatgggaa agatcggagg agagtccagc gtcgcgatcg acgccccgat
      481 cgaggcggtc tacgcggtcg ccgccgacgc cgagggcgcg ccccgctggc agccggagat
      541 cgaggtcgcc gagtgcctcg agcgcgacgg cgcgggcgac cagctgcggg tccggatcga
      601 gaccgaaacg ccgatcaagc gcctcacctc ggtcctcgtc tacagctacg agtccccgac
      661 ccggatcagc tggcgccagg aggacggaga cctcaagtcg gtcgagggca gctgggagtt
      721 gcgcgacctc ggcgacgagc gcaccgaggc cacctacaag ctcgaggtcg accccggccg
      781 gatcctcggc atggccctcc gcggccccgt cgtcggcatc ctccgcggca agctggtcga
      841 ctcgatgccc ggcaagctga aggcgttcct ggagcgttag ctcacgaact caggtgttgc
      901 gctgcggcgc cgacgctgca aggatcggcc gatgagcacg gtcaccgcgg gctcattgga
      961 ggagtggcag gaccgggtcc gtcgccagac ggcggtcgag ctgcgcccga acctggacgc
     1021 gctcgtgggc gagtgcgtcg actacgtaca ggactcgctg ccccagctcg acgtcgacga
     1081 ggagctggcc gacgggctct atgagagcgc ccgcgacaac ctcgaccgga tcttcgggat
     1141 gctggcccag gggatcccgg tcagcgacac cgtggcaccg ccgggcgcga tccgctacgc
     1201 cgagatcatg gtccggcgca acacgccgct ggccaccctg ctgcgcgcct accggctcgg
     1261 gacgatgtgg ttctggaccc tctggcggcg cgagatcgtc aagcggatcg acgagccgga
     1321 gctgctgatg caggcggtcg accactcgat gtcgttcgtc ttcgactacg tcgacctggt
     1381 ctccgagcag gtcaccgagg agtacgcgct gcagcgctcg cgctgggcgc gctcggcggc
     1441 ggcgctgcgc cacgagacgg tcgagggcct gctcgaaggc tcgctgaccg acgtcgacgt
     1501 cgccggctcg cggctcggct acgacctccg ccccacccac ctggccctgg tcctctccgc
     1561 cagcccgaaa agcgacaccg aggacgtcgt cgcccggctc gagcactgcg cgcaggagct
     1621 ggcggcgcgg gtgacgccgc ggcgaccgct gctggtcccc gacgggcgca cgacgatgtg
     1681 ggcctggctc tccttcgagc acgcgccggg cggcgacgtg ctggccgagg tcgagcagga
     1741 ccgcaagctg ctcgccgaag tctgcgtcac ctgcggtacc ccgcaggccg ggctcgccgg
     1801 cttcgtcgcc tcccaccagg aagcgctgca cacccgcgag ctgatgcgcc tctcaaaccg
     1861 cggccgtggc cgcctcgtcc gctacgacga agtcgcgctg gtctcgctgc tcgccgccga
     1921 cctcgaccgc gcccgcaact tcgtcgcccg cgagctcggc gagctgggca actccgacga
     1981 tgcgacgacg cggatccgcg ccaccgtgca ggtgctgctg gaggagagcg gccgcaccgc
     2041 ggtcgccgcc cgccgcctcg gcgtccacca gaacaccgtc tcctaccggg tgcgccaggc
     2101 cgaggagatc ctcggccact cgctgaaaca gcgccgcttc gaggtggaga cggcactgcg
     2161 cctgcgcgac ctcctggtcg agtagccgcc gcgcggcgat tgtcggagtc ccacaaagga
     2221 gcggcggcga gactggtgag ttgtcacgag cttcgctgcg aggggtcggc ctagtgtcgc
     2281 ccccatgcag gcaatcacga tcgacatcga taccggcggc accttcaccg acgccttcgt
     2341 cgtccgcgac gggcacccgc atcgggtcaa ggtgctgacc accccccacg acttcgccct
     2401 ctgcttctcg caggtgatcg aacgggccgc cgaggtgctc gacctcgaca ccgccgagct
     2461 gctgcgcgag accgcgtgca tccgctacgc gaccacggtc ggcaccaaca ccgtgatcga
     2521 gcggaccggg ccgcggatcg gcctgatcgt cgaccccgag agcgcggccg gcggcgtccc
     2581 cgagggggcc tttgtcgagc cggagatggt caccgcggtt gcgcccgggg acggcgacgg
     2641 cgcggtggtc gaggtcgcca aggagctgat ggcgcgctcc gcccgcgggc tcgtctgcgc
     2701 gatgccgagc gacgccgacg gcgccgaggc cgagcgcgcg gtcctgcgcg gcttccatca
     2761 gcactaccca cggcactgcc tcgacgcggt gccgctgacc ctctcgcacg agatcgcccc
     2821 cgaccccgac gacgcccgcc gcacggcgac ggcgttgttc aacgcctacg tgcacgagga
     2881 cgtcgcccac tacctctacc gggccgagga ctggctgcgc gaccacggtt acacgcggcc
     2941 gctcctggtc gtccacaacg acggcggctg cgcccgcgtc gccaagacgg tcgccggccg
     3001 cacctacaac tccgggccga ccgccgggct gctcggcgcc gaggcgatcg cggcgctctg
     3061 tgggatcgac cacctgctga ccttcgacgt cggcggcacc agcctcgacg tcggctttct
     3121 cggcgagggc cgcgcccact tcaaggagca cggccgggtg accggcgtgg aggtctcctt
     3181 ctcgatgccc gacatccagg tcctgggagc cggcggcggc tcgatcgccc acgtcgacga
     3241 cggcgtgctc gcggtcggtc cgcagagcgc cggcgccaac ccgggcccgg cctgcttcgg
     3301 gctcggcggc agcgaggcga cggtcaccga cgccgacgtc gtgctcggga tcatcgacgc
     3361 gaaggacttc ctcggcggcc ggatggagct ccacgccgac gccgccaccg cggcggtcgg
     3421 gcggatcgcc gagcggctcg gcatctccac ggtcgaggcc gcggcccaga tccgctcgac
     3481 cctgcacgac cggatggcgg agcggatcgg cgccgagctg accgagcgcg ggctcgaccc
     3541 ggcccgcacg acgatgctcg cctacggcgg cggcggaccg acccacgcgg cgccagtcgc
     3601 cgcccggatc gggatcggga ccgtcgtcac cctgcccttc ggcgccgtct tcagcgcctt
     3661 cggcgcctcc tcggccgacg tgcgccacgt ctatttcgcg gttcccggca gcgacgccga
     3721 ggagctgctg cgccgtaccg cgctgctcga catgggcggc gagggcttcg ccccggagca
     3781 ggtcgagatc gagctcgacc gggtccatcg acacggcgcc gagcgcctgc ggctgaccgc
     3841 ggtcgccaag ctcgaccacc ccgcgctgga ggcgggcgcg gccgaggggc acgagcccga
     3901 gccgagcggc agccgcgaga tccactggcc gggcgagggc acggtgccca cgcccgtcca
     3961 cgacgcggcg gagctgcgcc ccggcgccgt ggtcagcggc ccggccgtgc tcgaggcgga
     4021 ggacacgacc tacgtcgtgc cgcgcggctg gacccaccgg gtcgacgggc acgggatcgg
     4081 ctggatgacc agaaccgcaa aggaggagtc ctgatgaccg cgccaaccgg agcccgcgag
     4141 gcgatcaccg agtacctcga gatcgatctc gagaccgaga cctggcgctg ccgccgctgc
     4201 gaccacgaga tcggctccgc ccgcgaggac tacaagcgcg gcctgctgct ctacgaccgc
     4261 gacccgcgtg agatccaccc ggcgaagatc gacgctccct actcctatgc cccggacccg
     4321 gagtggtgcc ggatggtcga gtgctactgc cccggctgcg ggacccagat cgaggtcgag
     4381 tacctgcccc ccggccaccc gatcacgcac gacatcgaga tcgacgtcga cgccctgaag
     4441 gtgcgctgat ggagatcaac gtcgacatcg gcggcacctt caccgactgc ttcgccagcg
     4501 acggcgacaa ggtcgcctcg gccaaggcgc tgaccaccca ccacgacctc tcgcacgggt
     4561 tcatgaccgc ggtcgaggac ctcgcggcca gcttcgcgct cgcccccgac gagctgctcg
     4621 gcagcaccga ggcgattcgc tacgcgacca cgctcggcac caacgcgctg atcgagcgca
     4681 ccggcccgcg gctcggcctg ctgaccacga ccgggttcga ggacgtcgtc ccgatcggcc
     4741 gctgccgcgc ctacggcgag ggcctctcgc acgtcgacaa ccgcaacctg gcccgcgctc
     4801 agcggcccga gccgctgatc ccgcgcgagc tgatcgccgg cctgcgcgag cgggtcgacc
     4861 aggccgggca ggtggtgatg ccgctcgacc ccgacgaggt gcgcgaaaag gtgcgcgagc
     4921 tggtcgaccg cggtgtccgc ggcttcgtcg tctccttcct ctggtcgttc aagaaccccg
     4981 agcacgagct gctcgtgcgc gagatcatcg aggaggagta cccgtcctcc tacctcggcc
     5041 gcttcccggt gatcctcgcc cacgaggtct ccagccgctc cggcgagtac gtgcgctcga
     5101 tgacctcgat cctcgacgcc tacctgcacc gcttcaccgc cgaccggatg tcggacctgc
     5161 gcttcaagct gcgcgagcgc ggctacggcg gcccgctgct gctcgtccac aactccggcg
     5221 ggatgggcgg cctggcgcgg accacgccgt tgcagacgat ccacgccggt ccggtcgccg
     5281 gcctctacgg ctcccgccac atggccagcc gcttcgagct cggcaaagtc gtcaccaccg
     5341 acatgggcgg cacctccttc gacatcggcg tcgtcgtcga gggctcggtg cgcttttacg
     5401 agttcaaccc ggtgatcgac cgctggctcg tgcagatgcc gatgatggac atcaaggcgg
     5461 tcggcgccgg cggcggctcg atcgcctcct acgacggctt ccgcggcgtc gcggtcggcc
     5521 cgcgcagcgc cggctccacc cccggccccg cctgttacgg ccagggcggg accgagccga
     5581 ccgtgaccga cgccaacctg gtgctcggct acctcaaccc cgactactac cacggtggtt
     5641 cgatggcgct cgacacgcgc cgggccgagc gcgcgatcga gcgccgggtg gcgcggccga
     5701 tggaggcctc ggtcgccgag gcggcgcgct cgatccgcgc cgtcgtcgac aacaacatgg
     5761 gcgacgcgat cttcaaggag atcgtgctga agggctatga cccgcgcgac ttcaccgtct
     5821 tctccttcgg gggcggcggg ccgctgcacg cctgcagcta ctcggaagtg ctgcagatcc
     5881 cgcgcgtcgt cgtcccgccc cactcgccgg tcttctcggc ctcgggagcg gccgggctcg
     5941 acctcctgca catctacgag cgctccgagc acatcgtcct gctcaacccg ctgttcaaga
     6001 ccgtgctcga ggactacggc cagatcaacg acgtcgtcga ccagttcatg gccgaggccc
     6061 gcgaggactt cgccgacgag ggccacggcg aggacgcgat cgagtacgcg ctggagctcg
     6121 acgtgcgctc cggcggccag ctctacctga ccacgatcaa gagcccgact ttgaagatcg
     6181 agtcgcggga ggacctcgcc gcggtcctgg aggcctactt caccgagtac gccgaccgct
     6241 tcggcgagtt cgcggtgacc cgcgaggtcg gcgcctcggt cgagacggtg cggctgcgcg
     6301 cttgggtcaa gcgccagcgc tgggagccgg agctgctgcc ggcgcccggg cacccggtcg
     6361 aggagacccg caagggcgag cgcgactgct ggtgggagcg ggccggcggc ttcgaaccga
     6421 ccccgatcta cgactacgac cgcctcgggc ccggccacga ggtcgccggg ccggcggtgc
     6481 tggagggccg cgacaccacc gtcgtcgtca accccggctg gagcgcggcg atggacgacc
     6541 acgggttcct gacgctgacc aacgaggagg agagagcatg aagccccccg agacgctgac
     6601 gccggcggcg ccggcgcggc ccgaggacgc ggaggcgttc ttcgccgagc tgctcaacga
     6661 caagccggtg atgtacgggc cctcgcccgg gatcatcgac gacaaccagc tctccccgcg
     6721 cagcgccgac gaggaggagg cgctgctggt cgccaccgac gaggtcgagc tgacgatcgc
     6781 ccagaaccgt ctggaggtga tcctcgagtc ggcgaaggag atgctcgagc agaccggggc
     6841 ggcgccggga gccaagtggg gcgacctcac ctgcggcctc tacagcgccg ccggcgacct
     6901 cgcgctcgcc tcctccagcg gcgtcgtcgt cttcgccgcc tgcgcctcgg cgccggtcaa
     6961 gcacatcgtc aaggactgga tcgaggagcc caccgtcggg gtccggccgg gcgacgtctt
     7021 ctaccacaac gatgcccgct acggaggggt ccacaacgcc gaccaatccc tgttcatccc
     7081 gatcttcgcg ggcgagcgcc tgatcgcctg ggcgggggcg acgatccacg agggcgagaa
     7141 cggcgcgacc gagcccggcg gcctctcccc cagcgccctc tcggtctacg acgaggggct
     7201 gaagatctcg ccgatcaagg tcgccgagaa ctacgtcttc cgccgcgaca tcgtcaacct
     7261 gatgcagaac aacgtccgcg acccgctgat gcagctgatc gacatgcggg cgaagctggc
     7321 ggcggcgatg cggatcgagg agcgggtgat ggaggtcgtc gccgatcgct ccgccgaggt
     7381 cctgctggcg acgctgcgca acgcgctgga gcggacccgg gccgaggtca agcggcgctt
     7441 cgccgagttc cccgacgggg tcttccgcgc ggtcgccttc tgcgacacga ccctgctcga
     7501 ggaccggatg ctgaagctgg agctgcggct ggagaagcgc ggcgacaagg tcgtctgcaa
     7561 cacccacggc agcggcccgg cgatccccga ccgggcgctg aacatggacc cgaccttcgc
     7621 ccgctcgctg ttctccaaca acctgatgaa cttcttctgg tcggacctgc cgcgcaacgc
     7681 cggctacgtc tcggcgatcg agttcgagtt cgaggagggc tcgatcgcca aatcgggcgc
     7741 cgagtgcccg aacgcgctct cgatggtcgc cgcctgcttc taccagacgc tgatgcacgt
     7801 ctgcatgtcg aaactggcct tcaaccggcc cggcgacgtg cagatcatcg ccccctggta
     7861 cgggatgacg caggcgatgt tctacggcgg gctgaaccag tgggggatgc cggtcgccaa
     7921 cctgatgatc gacatcaact cgatgggcgg cggcgctcac cccgaccgcg acggcgagca
     7981 ctcctgcgcg cccttcttcg cctcgatggc cgactacggg gagatcgagg accgtgaaac
     8041 cgagctgccg atcctcgcgc tcgggcgcag cctgaccgtc gacaaccacg gcttcggccg
     8101 ccaccgcggc ggctcctcgg tcgagtgcat gtacatggtc tggggagtgc cggagttcgc
     8161 cttcggctcg ctcgcgaccg ggagcaaatt cccgaccatc ccgggcctgt tcggcggcta
     8221 cggcggcccc agctcgccgc tgaccgcgtt caaagcagag agcccggagg cggtgaaggc
     8281 ggcgatgcgc gaggcccccc acgcggtccc gaccaccgcc gacgagctgc acgcgcacgg
     8341 cgggctgccg ggcgaggcgg agacgcggcg cgccacccag accgccaact tcctcggcga
     8401 gggcgacctc tggatcctcc aggtcggcgg cggcggcggt tacggagacc cgctcgagcg
     8461 cgaccccgag ctggtgatgg ccgacctgcg ccagaagcgg atctccggcc cggtggccga
     8521 gaacgtctac cgggtcgtct acgacgccga gcggctggtg gtcgacgccg aggcaacggc
     8581 gggggcacgg gacgccgagc gccgggcgcg gctgacccgt ggcctgccct acgacgactt
     8641 cgtgcgcagc tggcggcgcg aggagccgcc gccggagatc cccttcctcg gccgctggga
     8701 ctgggaggcg cagcggtgag cgcggtcggc gaggccccgc cccggcacga cccggccgcc
     8761 cgccacggcg tcgcgatcgg cgagggcgcc gcggcgctcg atctgatgtc gacgatgcgg
     8821 gcgatgcgca agctgcgccc cgacccggtc ccgcgcgaga ccctcgagcg gctggtcgag
     8881 gcggcgacct gggcgccgag cggcggcaac acccaggagt actcgttcac gatcgtcgac
     8941 gatcgcgaga cgatggccaa ggtggcgaag atctggcgcc gcacctgcgc gtactacacc
     9001 gccgcccagc gccccctccc tcccccctac gtcaagcccg aggcctggga caaggtcatg
     9061 gactcgctcg aagtgctcgc cgacgacttc gaggagacgc cggccctgat cgtcgcctgt
     9121 tacgacatga gcgggatcct caagcggatg atcaaaggct ggaagcacac cctgcgcggc
     9181 gcccgcgcga tcggggtctg gcagacgctg cgcttcctcc cccgcattcc ccgcttcatg
     9241 agcaccggcg aggcggcctc gatctacccc ggggtgcaga acatgctgat cgccgcccgg
     9301 gcgctcgggc tcggggcgac gctgaccacc tggcacacgc tctacgaagg cgattaccgg
     9361 cgcgcgctcg ggctctcgcg gcgcacgaag atctacgcga tcgtcccggt cggctacccg
     9421 cgcgggcact tcggcccggt ctcgcgccgg cccgccgccg aggcgatccg ctacaccggc
     9481 gaggcgacgg gctgagcggt gctgctgatc gacaccttcg accgcggggt cgcgcgcaac
     9541 cccgagggca cctgcttcct gaccgccgac gagtcggtgc ggatgagcta caccgaggtg
     9601 gcggcgctct cccaccgggt cgccgcggcg ctggccgcgc acggcgtcga ggccgagacc
     9661 cgggtcgggg tgctctcccc gaacgcgccg atcgggttct gctgcgtgct cggggcgctg
     9721 cggctgaacg ccagctgggt gccgctgaac gcgaaggcga cggcggccga cctggtgccg
     9781 ctgctcgagc tgaccgactg cgaggtgctc ttctaccacc cggcgctgct gccgctggcc
     9841 gaagcggtgc tcgagggctg cgacacggtg cgtctggcga tcgcgatcga ggagggcggg
     9901 cggcccggcg acccccggct cggcgagtgg atggcgcctg agggcaccac ggtcccgcag
     9961 ccgccgcacg agcccgaccg caccgcgatc atgttcggga ccggcggcac caccgggcgc
    10021 tcgaaggcgg tcccgatcac ccaccggatg gcggaagcga tgaacctcgc cttcgccggc
    10081 cacatgcccg agccgcagcc gccgacgatg gcgatggcgg cgccgatgac ccacgccgcc
    10141 gggccgatct gcttcccggt cttcgccgag ggcggcaccg tcgtcgtcca cgacggcgtc
    10201 gtgcccgacc tgctgctcgg ctcgatcgag cgccaccggg tcagccgcct gttcctgccg
    10261 ccgaccgcga tctacgccct gctctcggtg ccggacgtgc gctcctacga cttctcctcg
    10321 ctgcgccact tcatctacgc ggcggcgccg atggcggtcg acaagctcga ggaagccctc
    10381 gacgtcttcg gcccggtgat gacccagact ttcggccagg ccgaggcgcc gatgatctgc
    10441 acggtgatga cgccggcgga gcacgtcgcc gccgccgccg acccggcgct gcgcaaccgt
    10501 ctcgccagcg ccggccggtc ctcgctggtc gccgaggtgg cgatcatgga cgacgacggc
    10561 gggctgctgg ggccgggaga ggtcggcgag atcgtcgtcc gcggctccct ggtgatggcc
    10621 gggtaccaca aggaccccga gcagagccag gccacccgcc ggcccggcgg ttggcacggg
    10681 accagcgacg tcggcaggat cgacgagcac ggctacgtct tcatcgtcga ccgcaaacgg
    10741 gacatgatca tcaccggcgg cttcaacgtc tggccgagcg agatcgagca ggtgatccac
    10801 accgtcgagg gcgtccgcga ctgcgccgtg atcggcctcc ccgacgagaa gtggggcgag
    10861 gccgtgaccg ccgtcgtcga gctgcgcgag gacgcccccg ggatcgaggc cgaggccgtc
    10921 accgccctct gccgcgagca gctcggcccg gtcaagaccc cgaagcaggt gatcttccgc
    10981 gagctccccc gcagccccaa cggcaaggtc ctcaagcgcg ccctgcgcga cgagtactgg
    11041 accgaccggg cccgcgcggt ttagcgggcg tacatcgtca cgacggcggc gccgccgagg
    11101 ccgatgttgt gctggagggc gaccttggcg ccggcgacct ggcgggcctc ggcctcgccg
    11161 cgcagttgcc aggtcagctc gctgcactgg gcgaggccgg tggctcccag cgggtggccc
    11221 ttggagatca ggccgccgga ggggttgacg acccagttgc cgccgtaggt ggtggcgccg
    11281 tcgtcgatca ggtctccccc ctcgccctcg ccgcagaggc cgagggcctc gtaggtgatc
    11341 agctcgttga ccgagaagca gtcgtgcagc tcgatcacgt cgacgtcctc gggaccgagg
    11401 ccggtgcggt cgtagacgga gtcggcggcg gcgcgggaca tctcggcgcc gaccagggcg
    11461 atctcgtcgc ccgcgaaggt gctgggcagg tcggtgacca tcgcctgggc gacgatctcg
    11521 acggcgcggg cctcgaggcc acgctcggcg acgaaccgct cggaggcgat cagcgccgcg
    11581 gcggcgccgt cggaggttgg cgagcactgc agcttggtca gcggctcgcc gtaggaggtc
    11641 gaccccagca cctcctccag cgtgcaggga tcgcggaact gggcgtaggg gttgtttccc
    11701 gagtgcagat ggttcttgac cgcgatccgg gcgaactgct cttcggtcgt cccgtagcgg
    11761 cgcatgtgct cacggccggc gccgccgaac agctccaggg ccatcgggcc gccgcacccg
    11821 ggcgagagct ccgccgagcg ctcgacgaac cggtcgaagg gcgagacgcg atcggggaag
    11881 gccgactccg gcagcgagcc gcgggccatc ttctcgaagc cgagcgcgag cacgcactcg
    11941 gcgagcccac cggcaaccag ctggcgcgcg tagaagagcg cggtcgagcc ggtcgagcag
    12001 ttgttgttga cgttgacgac cgggatcccg gtcgtcccca gcgtgtagac ggccgtctgg
    12061 ccggcggtgg agtcgccgta gacgtagccg acgcaggcct gctcgacctc gccgtaggcg
    12121 agaccggcat cggcgagcgc ccgcgagccg gcttcgcgcg ccatctccgg gtagtcgcgc
    12181 tcgccccttc cgggcttctc gaacttggtc atgccgacgc cgacgacgaa tgctttgttc
    12241 atgccgcgaa gcctcgcgag cgcagccccc cggcgtttgg agcatccgcc caatccggcc
    12301 gggcaccatt tggcgattcg agccaggcgc gaacagggcg ctgcgcctag cgttgctggc
    12361 actgtgagcc gccgcgcgcc gatactccag gaggtcgcgg cacggatggc cgaacggccg
    12421 gcctcggacc tgcgcgagga tccggtcggc tgggcctacg cgctgcgcga cgcggcggcg
    12481 ctca
//
LOCUS       DASRIG010000207         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1408822, whole genome shotgun sequence.
ACCESSION   DASRIG010000207 DASRIG010000000
VERSION     DASRIG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408822"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VFB52_05725"
     CDS             complement(<1..2020)
                     /locus_tag="VFB52_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05866.1"
                     /translation="MRRDWQQQRTGKQGTSDSDPGRLGPIGRLGYAAATHRRAVALVW
                     VVVALVLGFLAPRVEHALSGAGWQAEGSDSVAAREQIDAEFGGASAYALQVAVHSPKL
                     TAADPEFRRSVAAVEAVLADSPDVSAVLPPQPGISLSRDGHTAIVTGSAAANPNRMVA
                     VAGELKAEISAAAAPGVETNLTGAAAMWSDFNEANKEAMLKSELISWPVTLAILVLAF
                     GSLVAAGLPLMLTITGLVASAGVLYLGTLVSPISIWALNFALMFALALGIDYALFIVM
                     RFRNALFGERLEPELAVARTMDTAGKAVLFSAITVLISLTAVMLVPSPAFRSLALGIM
                     IAVLFVLAATLTLLPAVLAKLGPRVDRLALPWAHGGDHRSERFARWGERLWRRPVLFG
                     GLALVALVALALPVLGLKTGMPSIKVVPEGDSSRVGYEQISAGFGPGAPGTLQVVAPG
                     AEASRTSAVLAADPGIAGVAPAAPGSGEAVLIAATAAAGPSTDAFGVTVERLRDKLPD
                     GVLVGGPAAENHDLDEALARTTPLAIGIVLALGFVLLLVAFQAPLLALIGVLANLLST
                     AAAFGVAKLVFQDGHLSGLLGFESQGYLDAWGPVFFFAMIFAIAMDYTVFLLSSAKEH
                     FDRSGDPQQAMMGGVAHSGRVIFAAAGVMIAVFFTFALSGPLPPKEMGV"
     gene            2095..2427
                     /locus_tag="VFB52_05730"
     CDS             2095..2427
                     /locus_tag="VFB52_05730"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO05867.1"
                     /translation="MQPTDLNPEAAELIARRFRALADPTRLRILNLLRNQDEASVGEI
                     ATKVGGSQQNVSKHLAALHAEGFVARRKQGTSSIYRIGDPGVHQICDGISAGIEAQLA
                     ELEAVLAR"
     gene            complement(2424..3182)
                     /locus_tag="VFB52_05735"
     CDS             complement(2424..3182)
                     /locus_tag="VFB52_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HZO05868.1"
                     /translation="MDRAASRHRREERWRLRAERARDAFGLVLVLVLLNYVLTSLLDN
                     RGWTAVLISLSVSATSIVALASSHSQPQAVRAAIWVSALVTLFTVIAATTETHLWLNF
                     ASGIQIVLLAVAMAAVLRRVVTTAEVGSRTILGALSVYAVLGLIFTYLYGTIDRLQST
                     PFFEGHQTPDGSDFIFFSYTTLTTTGYGNLVPDGQPGEMISGLEMMIGQIFLVTLVAG
                     LVSLWRPGEGLKRRLEKKRGGEIPGQPPASDSAS"
     gene            complement(3398..3985)
                     /locus_tag="VFB52_05740"
     CDS             complement(3398..3985)
                     /locus_tag="VFB52_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05869.1"
                     /translation="MGWFNRKSEERQRRLEATEREDEALAKAKAADPNSQRKTEFHNA
                     SVRGDLDRASSLLDDYLTEYEVDPGPTEYDRGRPSLLERAAQARRATKLGDLDEAASI
                     LAAWEIKPPPTSTTGPERFAQLPNLNTLGMLLIDFFSLPGAESNSQYDLLLDTKLRQV
                     AQWTRLSSPQPRPGGFFDQAYGQFKSKRELSRARS"
     gene            complement(4063..4149)
                     /locus_tag="VFB52_05745"
     tRNA            complement(4063..4149)
                     /locus_tag="VFB52_05745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4113..4115),aa:Ser,seq:tga)
     gene            4213..>5357
                     /locus_tag="VFB52_05750"
     CDS             4213..>5357
                     /locus_tag="VFB52_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05870.1"
                     /translation="MRRLLILASTMIFLDVVFFSAIAPLLPEYADDLGLSDAQAGILS
                     AAYAAGTLLAALPGGWVASQVGPRKTTIVGLGLLGVSSLAFGFAEQIVLLDVARFCQG
                     VAGALVWSGALTWLITTAPEEKRGSVIGTALGTAVAGALLGPALGALAGSIGTEAVFA
                     SVLVISLILAGFAARMPEAGTPERQSLRIVAATMVTRPIVLGVVFVAVPSVMFGAVEV
                     LVPLRIDDLGGSHGVIAAGFIAGAALEATLAPLAGRISDRIGRRTPYVAGLVVCAAAM
                     VGVAAAQVLGGVLAALIVTSLGAGLCFAPALTLLSDAAEHTDLHQGFAAGLSNMAWAS
                     GQVIGGVGGGVVATLTGDAVPCIAIALLLVGTALFSFRTLPAAPTVSV"
BASE COUNT          784 a         1814 c         1943 g          816 t
ORIGIN      
        1 tcactcccat ctccttgggc ggaagcggac ccgagagcgc gaaggtgaag aagaccgcga
       61 tcatcacgcc tgcggcggcg aagatcacgc ggccggagtg cgcgacgccg cccatcatcg
      121 cctgctgggg gtcgcccgag cggtcgaagt gctccttcgc agaggagagc aggaacacgg
      181 tgtagtccat cgcgatcgcg aagatcatcg cgaagaagaa gaccggcccc caggcgtcga
      241 ggtagccctg cgattcgaag ccgagcagcc cgctcaggtg cccgtcctgg aagaccagct
      301 tggcgacgcc gaacgcggcg gcggtcgaga gcaggttggc gagcactccg atcagggcca
      361 gcagcggggc ctggaaggcg acgagcagga ggacgaagcc gagcgccagc acgatcccga
      421 tcgccagcgg ggtggtgcgc gccagcgctt cgtcgaggtc gtggttctcg gcggcggggc
      481 cgccgacgag cacgccgtcg gggagcttgt cgcgcagccg ctcgacggtc acgccgaagg
      541 cgtcggtcga cggcccggcg gcagcggtcg cggcgatcag caccgcctct ccggatccgg
      601 gcgcggcggg ggcgacgccg gcgatgccgg ggtcggcggc gagcacggcg gaggttcggg
      661 acgcctcggc accgggtgcg accacctgca gcgtgccggg ggcgccgggg ccgaagccgg
      721 cgctgatctg ctcgtagccg actcgcgagg agtcgccctc cggcaccacc ttgatcgagg
      781 gcatcccggt cttcagcccg agcacgggca gcgccagggc caccagcgcg accagggcga
      841 ggccgccgaa cagcaccggc cgccgccaca accgctcgcc ccagcgggcg aagcgctccg
      901 agcggtgatc gccaccgtgc gcccagggca gcgccagccg gtcgacccgg ggaccgagct
      961 tggccaggac cgcgggcaac agggtgaggg tcgcggccag gacgaagagg accgcgatca
     1021 tgattcccaa ggccagcgag cggaaggccg ggctcggcac cagcatcacc gcggtcagcg
     1081 agatcagcac ggtgatcgcc gagaagagga ccgccttgcc ggcggtgtcc atggttcgcg
     1141 ccaccgccag ctcgggctcg agccgctcgc cgaagagcgc gttgcggaac cgcatcacga
     1201 tgaacagggc gtagtcgatc cccagcgcca gcgcgaacat cagcgcgaag ttcagcgccc
     1261 agatcgagat cggcgagacc agggtgccga ggtagagcac gccggcggag gcgaccaggc
     1321 cggtgatcgt cagcatcagc ggcagcccgg cggcgaccag cgagccgaag gcgaggacga
     1381 ggatcgccag ggtgaccggc caggagatca gctccgactt caacatcgcc tctttgttgg
     1441 cttcgttgaa gtccgaccac atcgcggcgg cgccggtcag gttcgtctcc acgcccggcg
     1501 ccgcggcggc cgagatctca gctttcagct cgccggcgac cgcgaccatc cggttcgggt
     1561 tggcggcggc gctgccggtg acgatcgcgg tgtggccgtc gcgggagagg ctgatgccag
     1621 gctgcggcgg tagcaccgcg ctgacgtcgg ggctgtcggc gagcaccgct tcgaccgcgg
     1681 cgacgctgcg gcggaactcc gggtcggcgg cggtcagctt gggggagtgg acggcgacct
     1741 gcagcgcgta ggcgctggcg ccgccgaact ccgcgtcgat ctgttcgcgg gcggcgaccg
     1801 agtcggagcc ttcggcctgc cagccggcgc cggagagggc gtgctcgacc cggggagcga
     1861 ggaagccgag gacgagggcg acgacgaccc agacgagcgc gaccgcgcgt cggtgggtcg
     1921 cggccgcgta gccgagccgc ccgatcgggc cgagtcgacc cgggtcactg tcgctcgtgc
     1981 cctgtttccc ggtccgctgc tgttgccaat ctctgcgcat cgcgtgcccc cagttctcaa
     2041 ctttacaagt tcacaactgc acaacttaac agttgtagac ggtacaataa cacgatgcag
     2101 ccaaccgatc tgaatcccga ggcagccgag ctgatcgcgc ggcgctttcg cgctctcgcc
     2161 gacccgaccc ggctgcgcat cctcaacctg ctgcgcaacc aggacgaggc ctcggtgggg
     2221 gagatcgcga ccaaggtcgg cggctcccag cagaacgtct ccaagcacct cgccgccctc
     2281 cacgccgagg gcttcgtcgc ccgccgcaag caggggacga gctcgatcta ccggatcggc
     2341 gaccccggcg tgcaccagat ctgcgacggg atctcggccg ggatcgaggc ccagctggcc
     2401 gagctcgaag cggtgctggc gcgttaggaa gcggagtcgg acgccggcgg ctggccggga
     2461 atttcgccgc cgcgtttttt ctccagccgc cgcttcagcc cttcgccggg acgccagagg
     2521 ctgaccaggc cggcgaccag ggtgacgagg aagatctggc cgatcatcat ctcgagaccg
     2581 gagatcatct cgcccggttg cccgtccgga accaggttcc cgtagccggt cgtggtcagg
     2641 gtcgtgtagc tgaagaagat gaagtcgctg ccgtcggggg tctggtgtcc ttcgaagaag
     2701 ggcgtgctct gcagccggtc gatcgtcccg tagaggtagg tgaagatcag gccgagcacg
     2761 gcgtagacgc tgagcgcgcc gaggatcgtg cgcgagccga cttcggccgt ggtcaccacc
     2821 cggcgcaaga ccgccgccat tgccaccgcc agcagcacga tctggatccc tgaggcgaag
     2881 ttgagccaga ggtgggtctc ggtggtggcg gcgatcacgg tgaaaagcgt gaccagtgcc
     2941 gagacccaga tcgccgcccg caccgcctgc ggctgcgagt gcgagctggc cagcgccacg
     3001 atcgacgtcg cgctgaccga gaggctgatc agcaccgcgg tccagccgcg gttgtcgagc
     3061 agtgaggtga gcacgtagtt gagcagcacc aggaccagaa ccagcccgaa ggcgtcccgc
     3121 gcccgttccg cccgcagccg ccagcgctcc tcgcggcggt ggcggcttgc cgctctgtcc
     3181 atgcacggac gttcggctct gccaagcggt ttcctgccca tcatcgggcg tgggcgcgat
     3241 gttggtgtga gcgaaatcgc tgaacttggc tccacgcgcc cggggggcag gccgagatcg
     3301 aggcctggct cgccgccgct ggcttcaagt cactgcggtt cgagcgctcg ggtccgatgc
     3361 tgttcttcga ggaccgcctg cggagtggag tgcccgttta gctcctggct cgggacagct
     3421 cgcgcttgga cttgaactgg ccgtaggcct gatcgaagaa tccgccaggt cttggctggg
     3481 gggaggaaag ccgggtccac tgcgcgacct gccgcagctt cgtgtcgagc agcaagtcgt
     3541 actgtgagtt gctttctgct ccagggagcg agaagaagtc gatcagcagc atgccaagcg
     3601 tgttgaggtt cggcagctgc gcgaagcgct ccgggccggt cgtcgaggtc ggcggcggct
     3661 tgatctccca ggcggcgagg atgctcgccg cctcgtccag atctccgagt ttggtcgccc
     3721 gccgcgcctg cgctgctcgt tccaataagg acgggcggcc tctgtcgtat tcggtcgggc
     3781 ccgggtcgac ctcgtactca gtcaggtaat cgtcgagcaa gctcgaggcc cggtccagat
     3841 cgcctcggac cgaggcattg tggaactcgg tcttccgctg ggagttcgga tcggcagcct
     3901 tcgctttcgc cagcgcttcg tcttcgcgct ctgtcgcttc cagacgcctc tgtcgctctt
     3961 ccgactttct gttgaaccaa cccatgatcg cccccgtctc gtcggtggat gccgacgcta
     4021 tccgagcggc tgggcagggg aaccttcatc cgagttctct aacggagggg gtgggattcg
     4081 aacccacgag cgccttgcgg cgccactggt tttcaagacc agcgcattca accgctctgc
     4141 cacccctccg gaggagctgc gcggcgaatg ctagaggccg gccgggggct gggcgcctga
     4201 ttggatggag cgatgcgccg cttgctgatc ctcgcctcga cgatgatctt cctcgacgtc
     4261 gtcttcttct cggcgatcgc tccgctcttg cccgagtacg ccgacgacct cgggctgagc
     4321 gacgcgcagg cggggatcct ctcggccgcc tacgcggcgg ggacgctgct ggcggcgctg
     4381 ccgggcggct gggtcgcgag ccaggtcggg ccgcgcaaga cgacgatcgt cgggctcgga
     4441 ctgctcgggg tctccagcct cgccttcggc ttcgccgagc agatcgtgct gctcgacgtg
     4501 gcccgcttct gccagggggt cgcgggggcg ctggtctggt cgggcgcgct gacctggctg
     4561 atcacgaccg cgccggagga gaaacgcggc tcggtgatcg ggaccgcgct cggcaccgcc
     4621 gtcgccggcg ccctgctcgg gccggcgctg ggcgcgctcg ccggctcgat cgggaccgag
     4681 gcggtcttcg cctcggtgct ggtgatctcg ttgatcctgg cggggttcgc cgcacggatg
     4741 cccgaggcgg ggacgccgga gcgccagagc ctgcgcatcg tcgcggcgac gatggtcacc
     4801 cggccgatcg tcctcggggt cgtcttcgtc gccgtgccct cggtgatgtt cggcgcggtc
     4861 gaggtgttgg tgccgctgcg gatcgacgac ctcggcggca gccacggcgt gatcgccgcc
     4921 ggcttcatcg ccggcgccgc gctggaggcg accctggcgc cgctggccgg gcggatctcc
     4981 gaccggatcg ggcgccgcac gccctacgtc gccgggctcg tggtctgcgc cgcggcgatg
     5041 gtcggggtgg cggcggcgca ggtgctcggc ggcgtgctcg cggcgctgat cgtcacctcg
     5101 ctcggcgccg gcctctgctt cgcgccggca ctcaccctgc tctccgacgc ggccgagcac
     5161 accgacctgc accagggctt cgccgccggg ctctcgaaca tggcctgggc ctcgggccag
     5221 gtgatcggcg gcgtcggcgg cggggtggtg gcgacgctga ccggcgacgc ggtgccctgc
     5281 atcgcgatcg cgctgctgct ggtcgggacc gcgctgttct ccttccgcac cctcccggcg
     5341 gctcccacgg ttagcgt
//
LOCUS       DASRIG010000208         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3491357, whole genome shotgun sequence.
ACCESSION   DASRIG010000208 DASRIG010000000
VERSION     DASRIG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3491357"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..889
                     /locus_tag="VFB52_05755"
     CDS             119..889
                     /locus_tag="VFB52_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO05871.1"
                     /translation="MSNAEYLQENQPPGVPPLPLTGERTLPDVPEENYWYRRHLVVYE
                     WIAERTAGRRVVDMACGEGYGSDVLARRAAGVVGVDANPEAHEHARLRYTRPNLRFER
                     DLVESFAEPCDAVVFLQTIEHVQDAGAILEHFKSMLDPGGVAYVSTPNLLTLAPPGAE
                     KSDNPWHVHEYRAEEFRELCAAHFPEVELLGLFHARKLRAHELAIKLGWDTVHKRLGL
                     TKRFYDRFTPAIAASDFSLRAGDLDKALDFLAVCRLPA"
     gene            886..2298
                     /locus_tag="VFB52_05760"
     CDS             886..2298
                     /locus_tag="VFB52_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HZO05872.1"
                     /translation="MSRSDSAGDLALVLHSHMPYVEGFGTYPFGEEWLFDAVIRSYLP
                     VLEVARDLTLTVTPVLADQLEDAGARERLRDFLVEWRIGAAEADLREVPAECRPACEG
                     ELARYSRALELLDAEGGDPLRPFQRAHAEGRVALATSAATHAVLPLLATRAGISLQLD
                     AGIRSHRRRFGWEGGVWLPECAYEPGLEWRLAENGAEWFCVDQSAHQEPFEALAPVRT
                     PAGPVALPIDWEAISWLWSLDGYPSDPSYAEFEGKSLRGIRIWKIGGGAYDPAAAAAA
                     ARRQAAEFLTAVAARLRAYESERGRRGLLVFAIDTELLGHWWSEGPLWLGEVLSGAEQ
                     AGVRLVDVPTALAEHEPVERPLAASTWGEDKDLHTWDSPPVADLAWAARRMELRLLRA
                     LGNGLRGEAAQRAARELLSVQASDWAFLDQRGQAGDYAYQRATDHARAMLEAIDSPFD
                     TDSRMRSIAPDMSLAPLLEP"
     gene            2309..3502
                     /locus_tag="VFB52_05765"
     CDS             2309..3502
                     /locus_tag="VFB52_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO05873.1"
                     /translation="MNRILILSWEYPPLIEGGLARHVRKLSEALVELGIDVHVLTRGG
                     EESPAEQRSNGVVVHRIREPKRPTDLGEFVAWVERMNSDMLAAGVELGDRYRYDVVHG
                     HDWLVANACDHLAKRFGAPLVTTIHATEYGRHQGWVDKHPQTYIHGVERWIVNRSQRT
                     IACSHYMREQIVDIFGAQEDLVSVIPNGIDPEDLPAQHPAELARLRAEFAAPDEKLVL
                     LIGRLVYEKGFQLALEAMPRVIEEAPGTRFLVAGSGTHEAELHRQAEELGLMEHGTFL
                     GWIGDDVLHSLYRISDLTVVPSIYEPFGLVALEAMASGCPCIVADTGGLREVVPHEGA
                     GLRFAAHDPEALGEVAVRVLTDEELSRRLVAEAHEHVLRFDWADVAARTAEVYADLVG
                     ETSAA"
     gene            complement(3505..3801)
                     /locus_tag="VFB52_05770"
     CDS             complement(3505..3801)
                     /locus_tag="VFB52_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO05874.1"
                     /translation="MEIAVRFGNNLVRARKRAGLSQEEVGFRASLHRTEIGMLERGTR
                     LPRIDTLVKVAAAVGVEPSELLEGLDWTAPAMPTTGSFSVRDDPEPDEPDPNLN"
     gene            complement(3963..4610)
                     /locus_tag="VFB52_05775"
     CDS             complement(3963..4610)
                     /locus_tag="VFB52_05775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZO05875.1"
                     /translation="MIGFLAKNKMLNHRYGLLLWRYSWRRWLTRAGRRWQTDGFVFLG
                     RRLELQIGKQGTIRFGRFVWIGDGSKIRCHEGEVEIGRKTVMGQECTISAYQRVKIGE
                     QCVIADRAMFIDFDHGVVEVERPIRLQGIYKRDVEVGSNVWIGYGACVLRGVRVGDNA
                     VIGTNAVVTKDVPANAVVGGIPARIIRMREAPTQLRWPDPVEPDPDAHTTLQPPG"
     gene            4638..5600
                     /gene="meaB"
                     /locus_tag="VFB52_05780"
     CDS             4638..5600
                     /gene="meaB"
                     /locus_tag="VFB52_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZO05876.1"
                     /translation="MTDTAKTAPLAERLVAGDKRALARAITLIESDDPAGWELVREVY
                     PRTGNARIVGFTGPPGVGKSTLIGALTGELRKTDRAVAVLSIDPSSPFTRGALLGDRI
                     RLTDHFLDPGVFIRSMASRGALGGLSEAALQVALVMDAAGKDDVLIETVGVGQAEIDI
                     VDHADTIVLALMPGSGDSIQALKAGVMEIPDVIVVNKADHPMTDTMVREVRGVLALSH
                     DPDGWQVPILRTEAARGEGVEELAEKIAEHRAFIEDAGTLSERRARNLRSEVIGIATS
                     RMRRKLEETIAEDTGTAELLDQVVRRELDPASAASELLERHTDV"
     gene            5593..5997
                     /locus_tag="VFB52_05785"
     CDS             5593..5997
                     /locus_tag="VFB52_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZO05877.1"
                     /translation="MSEPAALPTLPEAMGWLGAAISAVDGDAVGQVQGFFVDTASGEP
                     AWLIARLGRRRNTRVVAVPMSNCAGAPFGVWVAQRGEALRGAPVVDPTRPLRREHELT
                     ICSHFEIGNRVGRAAEVTARPEGEITATPPGA"
BASE COUNT          888 a         2297 c         2142 g          893 t
ORIGIN      
        1 tgggcacgct gaacgcgatc gtcaagctca cctacacgcc gccgggcggc tcgccagcga
       61 ccctgcgcaa gcggctgacc ctcaagcagg actagcgcca gcgcaatacg gtgccggtgt
      121 gagcaacgcc gagtacctgc aggaaaacca accgcccggg gtgccaccgc tgccgttgac
      181 cggcgagcgg acgctgcccg acgtgcccga ggagaactac tggtaccggc gccacctggt
      241 cgtctacgag tggatcgcgg agcgcaccgc gggccggcgc gtggtcgaca tggcctgcgg
      301 cgagggctac ggctccgacg tgctcgcccg ccgcgcggcc ggcgtcgtcg gcgtcgatgc
      361 caacccggag gcgcacgagc acgcgcgcct acgctacacc cggcccaacc tgcgcttcga
      421 gcgcgacctg gtcgaaagct tcgccgagcc ctgcgacgcg gtcgtcttcc tgcagacgat
      481 cgagcacgtg caggacgcgg gcgcgatcct cgagcacttc aagtcgatgc tcgacccggg
      541 aggggtggcc tacgtgtcga ctccgaacct gctgaccctg gcgccgcccg gggcggagaa
      601 gtccgacaac ccctggcacg tgcacgagta ccgggccgag gagttccgcg agctctgcgc
      661 cgcgcacttc cccgaggtcg agctgctcgg cctcttccac gcccgcaagc tgcgcgccca
      721 tgagctcgcg atcaagctcg gctgggacac ggtccacaaa cgactcggcc tgaccaagcg
      781 cttctacgac cgcttcaccc cggcgatcgc cgcctccgat ttcagcctgc gcgccggcga
      841 cctcgacaag gcgctcgact tcctcgcagt ctgccgcctg cccgcatgag ccggagcgac
      901 agcgccggcg acttggcgct ggtcctgcac tcgcacatgc cctacgtcga gggcttcggc
      961 acctacccgt tcggcgagga gtggttgttc gacgcggtga tccgctccta cctgccggtc
     1021 ctggaggtgg cgcgcgacct gacgctcacc gtgaccccgg tgctggccga tcagctcgag
     1081 gatgccggcg cgcgcgagcg cctgcgcgac ttcctggtcg agtggcggat cggcgccgcc
     1141 gaggccgacc tgcgcgaagt gccggccgag tgccgccctg cctgcgaagg cgagctggcc
     1201 cgctacagcc gcgcgctgga gctgctcgac gccgagggcg gcgacccgct gcgcccgttc
     1261 cagcgcgccc acgcggaagg ccgggtggcg ctggcgacct cggcggccac ccacgcggtg
     1321 ctgccgctgc tggcgacccg ggcggggatc tcactgcagc tcgacgccgg gatccgctcg
     1381 caccggcggc gcttcggctg ggagggcgga gtctggttgc ccgagtgcgc ctacgagccc
     1441 gggctggagt ggcggcttgc cgagaacggg gccgagtggt tctgcgtcga ccagagcgcc
     1501 caccaggagc ccttcgaggc gctcgccccc gttcgcaccc ccgccggccc ggtggcgctg
     1561 ccgatcgact gggaggcgat cagctggctc tggtcgctcg atggctaccc ctccgacccc
     1621 agctacgccg agttcgaagg caagtcgctg cgcgggatcc ggatctggaa gatcggcggg
     1681 ggcgcctacg accccgcggc cgccgccgcg gccgcgcgcc gtcaagccgc cgagttcctc
     1741 accgccgtcg ccgcgcgcct gcgcgcctac gagtccgagc gcggccgccg cggcctgctc
     1801 gtcttcgcga tcgacaccga gctgctcggc cactggtggt cggagggacc gctctggttg
     1861 ggcgaggtgc tgagcggggc ggagcaggcc ggtgttcgcc tggtcgacgt gccgaccgcg
     1921 ctggccgagc acgagccggt cgagcggccg ctcgccgcct ctacctgggg cgaggacaag
     1981 gatctgcaca cctgggactc gccgccggtc gccgacctcg cctgggcggc gcggcggatg
     2041 gagctgcggc tgctgcgggc gctcggcaac ggcctgcgcg gggaggcggc tcagcgcgcc
     2101 gcccgcgagc tgctctcggt ccaggccagc gactgggcct tcctcgacca gcgtggccag
     2161 gcgggcgact acgcctacca gcgcgcgacc gaccacgcgc gggcgatgct ggaggccata
     2221 gactccccat tcgacaccga ttcgcgtatg cgttcgattg ccccggacat gagtctcgcg
     2281 cccctcttgg agccctgaga ctcacacgat gaaccgaatc ctgatcctct cctgggagta
     2341 tccgcccctg atcgagggcg gcctcgcgcg ccacgtgcgc aagctctccg aggcgctggt
     2401 cgagctcggg atcgacgtcc acgtgctgac ccgtggcggg gaggagtcgc cggccgaaca
     2461 gcgcagcaac ggcgtcgtcg tccaccgcat ccgcgagccc aagcgcccga ccgacctcgg
     2521 tgagttcgtc gcctgggtcg agcggatgaa ctcggacatg ctggccgccg gggtcgagct
     2581 cggcgaccgc taccgctacg acgtcgtcca cggccacgac tggctggtcg ccaacgcctg
     2641 cgaccatctc gccaaacgct tcggcgcgcc gctggtgacg acgatccacg cgaccgagta
     2701 cggccgccac cagggctggg tcgacaagca cccgcagacc tacatccacg gggtcgagcg
     2761 ctggatcgtc aaccgctcgc agcgcacgat cgcctgctcc cactacatgc gcgagcagat
     2821 cgtcgacatc ttcggcgcgc aggaggacct ggtcagcgtg atcccgaacg ggatcgaccc
     2881 cgaggacctg cccgcccagc acccggccga gctggcccgg ctgcgcgccg agttcgccgc
     2941 ccccgacgag aagctggtcc tgctgatcgg ccgcctcgtc tacgagaagg gcttccagct
     3001 ggcgctggag gcgatgccgc gggtgatcga ggaggcgccg gggacccgct tcctggtcgc
     3061 cggctcgggc acccacgagg ccgagctgca ccgccaggcg gaggagctgg ggctgatgga
     3121 gcacggcacc ttcctcggct ggatcggcga cgacgtcctg cactcgctct accggatctc
     3181 cgacctcacc gtggtcccct cgatctacga gcccttcggc ctggtcgcgc tcgaggcgat
     3241 ggcctccggc tgcccctgca tcgtcgccga caccggcggc ctgcgcgagg tcgtcccgca
     3301 cgagggagcg gggctgcgtt tcgccgccca cgatcccgag gcgctcggcg aggtcgccgt
     3361 gcgcgtgctc accgacgaag agctgagccg ccgcctggtc gccgaggcgc acgagcacgt
     3421 gctgcgcttc gattgggccg acgtcgccgc ccgcaccgcc gaggtctacg ccgacctggt
     3481 cggcgagacc agcgcggctt agcgctagtt caggttcgga tccggctcgt cgggctccgg
     3541 gtcgtcccgc accgagaagc tgccggtcgt cggcatcgcg ggcgcggtcc agtcgagccc
     3601 ctcgagcagc tcgctcggct cgactccgac tgcggccgcg actttgacca gcgtgtcgat
     3661 cctgggcaag cgtgtaccgc gctcgagcat cccgatctcg gtccggtgca gcgaggcccg
     3721 aaagcccact tcttcctggg agaggccggc tcgcttacgg gcgcgaacga gattgttccc
     3781 gaaacggacg gcgatctcca ccggccgcat tgttggttca ttttcccgtc gcggccacgc
     3841 tggatcggct ggtgagccca ctatctccat ccttactgtc tgctggcgag ccacgggaga
     3901 gatcgaggat tgcagacaaa aactctcgcg ttgttgagcg gaggctaacc cgcgctcgcg
     3961 actcagccag gcggctgcag cgtcgtgtgc gcgtcgggat cgggctcgac cgggtcgggc
     4021 cagcgcagct gcgtcggcgc ctcgcgcatg cggatgatcc gggccgggat gccgccgacg
     4081 accgcgttcg cgggcacgtc cttggtcacc accgcgttgg tcccgatcac cgcgttgtcg
     4141 ccgaccctga ccccgcgcag gacgcaggcg ccgtagccga tccagacgtt ggagccgacc
     4201 tcgacgtcgc gcttgtagat gccctgcagt cggatcggcc gctcgacttc gaccacgccg
     4261 tgatcgaagt cgatgaacat cgcgcggtcg gcgatcacgc actgctcgcc gatcttcacc
     4321 cgctggtagg cggagatcgt gcactcctgg cccatcaccg tcttgcgccc gatctcgact
     4381 tcgccctcgt ggcagcggat cttcgagccg tcgccgatcc agacgaagcg gccgaacctg
     4441 atcgtcccct gcttgccgat ctgcagttcg agccgccgcc cgaggaaaac gaacccgtcg
     4501 gtctgccagc ggcgccccgc gcgggtcagc cagcggcgcc acgagtagcg ccagagcagc
     4561 agcccatagc ggtggttgag cattttgttc ttggcgagga agccgatcat cgcgggccaa
     4621 ctctatgctg gacgcccttg acggataccg ccaagaccgc acctctggcc gagcgccttg
     4681 tcgccggcga caagcgcgcg ctggcgcgcg cgatcaccct gatcgagtcc gacgacccgg
     4741 ccggctggga gctggtccgc gaggtctacc cgcgcaccgg caacgcccgc atcgtcggct
     4801 tcaccgggcc gcccggggtg ggcaagagca ccctgatcgg cgccctcacc ggggagctgc
     4861 gcaagaccga tcgcgccgtc gcggtgctct cgatcgatcc ctcgagcccg ttcacccgcg
     4921 gcgccctgct cggcgaccgg atccggctca ccgaccactt cctcgacccc ggcgtgttca
     4981 tccgctcgat ggcctcgcgc ggcgcgctcg gcggcctctc cgaggcggcg ctgcaggtgg
     5041 cgctggtgat ggacgcggcc ggcaaagacg acgtcctgat cgagaccgtc ggcgtcggcc
     5101 aggcggagat cgacatcgtc gaccacgccg acacgatcgt gctggcgctg atgcccggct
     5161 ccggcgactc gatccaggcg ctcaaggccg gggtgatgga gatccccgac gtgatcgtcg
     5221 tcaacaaggc cgatcacccg atgaccgaca cgatggtgcg cgaggtgcgc ggcgtgctgg
     5281 cgctctcgca cgaccccgac ggctggcagg tgccgatcct gcgcaccgag gcggcccgcg
     5341 gcgagggagt cgaggagctg gcggagaaga tcgccgagca ccgcgccttc atcgaagacg
     5401 cgggcaccct gagcgagcgc cgcgcccgca acctgcgcag cgaggtgatc gggatcgcga
     5461 cctcgcggat gcgccgcaag ctcgaagaga cgatcgccga ggacaccggc accgccgagc
     5521 tgctcgacca ggtcgtgcgg cgtgagctcg accccgcctc ggccgcgagc gagctgctgg
     5581 agcgccacac cgatgtctga gccggccgcg ctgccgaccc tgccggaggc gatgggctgg
     5641 ctcggcgccg cgatctcggc ggtcgacggc gacgcggtcg gccaggtcca gggcttcttc
     5701 gtcgacaccg ccagcggcga gcccgcctgg ctgatcgcgc ggctcggccg ccgccgcaac
     5761 acccgcgtgg tcgcggtgcc gatgagcaac tgcgccggcg ccccgttcgg ggtctgggtc
     5821 gcccagcgcg gggaggccct gcgcggggcc ccggtcgtcg acccaactcg gccgctgcga
     5881 cgcgagcacg agctgacgat ctgctcgcac ttcgagatcg gcaaccgggt tggccgcgcc
     5941 gccgaggtca ccgctcgtcc cgagggcgag atcacggcaa ccccgcccgg cgcttagccg
     6001 ctcagcagct tttcgctaag agagccccga gcggtcaggt agcctgcgcg cggatgctcg
     6061 ctcggccaaa gctccgccgc accgtcgtcc cggtgctcgt ggtcgcgttt ctctgcctcc
     6121 cggcgaccgc cgcggcgctg accgtgacca agaccgtcga caccaacgac ggcacctgcg
     6181 acgccgactg ctcgctgcgc gaggcgatcg atgacacccc
//
LOCUS       DASRIG010000209         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3222771, whole genome shotgun sequence.
ACCESSION   DASRIG010000209 DASRIG010000000
VERSION     DASRIG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222771"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VFB52_05790"
     CDS             <1..1009
                     /locus_tag="VFB52_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05878.1"
                     /translation="KYDPARAKATPKGDPIGANLGAEGFARSAAAAISGFKAAETRWS
                     SFDRRPFCAKAVFAPAGETLKLKKGQTAQLGVQAKAQDGSTAKEAKWSLLKQVGADFT
                     PTASEAASANFSYTVKQAPGPFVEVTAKFTSTAGVGEDSWKQPTEALLPTRFTITFSG
                     SASYDASEVGEGGGQAQWSGNADLREMPSPYPPGTFPYELAMYKLNAGSITYSYSGSF
                     GKCSATASGSIDLAEQPDLVGGVVLTIFDKDPREYSVFLGMPLTAKATVPGLLGPCED
                     PEDEGAIDFFPGIGVPMLVNAPFPGGPVSPDWSFSGSGSSDFPGPDQSWQWSLIPSFG
                     T"
     gene            complement(1032..1565)
                     /locus_tag="VFB52_05795"
     CDS             complement(1032..1565)
                     /locus_tag="VFB52_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZO05879.1"
                     /translation="MGRLIATTQATVDGVIDPVGEWVQADGDHGEYSFERQARSAGLV
                     LGRKTYEGLAAYWPSQQGRWADMVNSMPKHVGSTTLSGDLEWNATLLDGDLGDSIPRL
                     KDEVDGDLFMHGSGEFAYALAERGLVDVYEVYLNPLVWGKGNVHVLGDRGTVRMELDD
                     VQRFDSGVVLLTYLPAS"
     gene            complement(1651..2415)
                     /locus_tag="VFB52_05800"
     CDS             complement(1651..2415)
                     /locus_tag="VFB52_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZO05880.1"
                     /translation="MATEKKIAVAGATGRLGRPTVEILRERGHEVVPISRSEGVDVIT
                     GEGLGAALEGVDLIVDAATGPSPDQAEATEFFTTAARNLQEAGAASGVEEILVVSIIG
                     TDHFNTGYNAAKVAQEQAHFNGPLPARVLRAAQFHEFVAELVEWGTEGDTARLPQMRT
                     QLVAAEVVAEALADLVDGADSAPIGNPIAEVAGPREENLLEAAKLLAAQRGDDLRIEA
                     GDFFDDPEGLYPAGAVLPGPIAKLAGPTYEEWLNQK"
     gene            2502..3389
                     /gene="sigJ"
                     /locus_tag="VFB52_05805"
     CDS             2502..3389
                     /gene="sigJ"
                     /locus_tag="VFB52_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HZO05881.1"
                     /translation="MTESGQKDLAARFEQNRDRLRAVAFRMLGASGEADEAVQEAWLR
                     LDRADPEVIEDLPSWLTTVVARICLDMLRARQARHEEPLGPHVPDPIVSPAAGGDPEH
                     EALVADSVGLAMLGVLETLSPEERVAFVLHDTFAVPFEQVADLLDRSPAAARKAASRA
                     RQRVEAEPTEPDVDLERQREVVDAFFAAAREGDFDALVAVLHPEIVLRSDGGEARPTA
                     NHVIRGAAEVAGRALTFAHLSPHVRPVLVNGVAGVLVAPEGKPFSVMAFTVAGGRVVA
                     IDALADPERLARLEFGLGT"
     gene            3423..4067
                     /locus_tag="VFB52_05810"
     CDS             3423..4067
                     /locus_tag="VFB52_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05882.1"
                     /translation="MAVKTALRLRFNRPSWALIGATLALAIAVLVPARADAFGTFANG
                     VLTVSGGEGKIVPRCASDGELTVSGVSVDDGPVYCRDLRRIEASSSISTLFDFSQLPD
                     DLGGGQGPIEIHATSTTSGKLEYASDKFIGAPGHINVFDGGLDFDSIVGGNLNDRLSG
                     GADSDKIEGGRGNDVLLGGSGADKLLGGPGRDTLKGGAGSDKLLGGPGKDTEKQ"
     gene            4099..4539
                     /locus_tag="VFB52_05815"
     CDS             4099..4539
                     /locus_tag="VFB52_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO05883.1"
                     /translation="MTPEDLENLAHLRRARDLIDREYARPLDVDEIARAALMSTAHFS
                     RRFRAAYGETPYSYLMTRRIEKAKALLRGGELSVTEVCMEVGCTSLGSFSARFTELVG
                     ETPTTYRARDHGRLAAIPSCFAKDITRPARKPSRNGETRAVPAP"
     gene            4550..4957
                     /locus_tag="VFB52_05820"
     CDS             4550..4957
                     /locus_tag="VFB52_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05884.1"
                     /translation="MNLTLSTCFVQVQDPDSALAFYRDQLGLEVRNDVGKGDYRWITV
                     GGADQPDVAIVLTNYVNGGPDDSDAVAALLAKGAMNGVHFHSDDLDGVFAKLQAAGVE
                     VVEEPKEQPWGTRDCAVRDPSGNLVRIDQPPAS"
     gene            4954..>5328
                     /locus_tag="VFB52_05825"
     CDS             4954..>5328
                     /locus_tag="VFB52_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZO05885.1"
                     /translation="MSKAKKGKSAAAPAADSHDLIRVHGARVNNLKDVSVEIPKRRLT
                     AFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPTLARPEVDVLEGLTTAITV
                     DQERMGSDTRSTVGTVTDANAML"
BASE COUNT          841 a         1868 c         1808 g          811 t
ORIGIN      
        1 caaatacgac cccgcgcggg ccaaagccac tcccaaaggg gatccgatcg gcgccaacct
       61 cggggccgag ggcttcgccc gcagcgccgc ggcggcgatc agcggcttca aggcggcgga
      121 gactcgctgg agcagcttcg accgccgtcc cttctgcgcc aaggccgtct tcgcgcccgc
      181 cggcgagacg ctgaaactga aaaaagggca aaccgcccag ctcggcgtcc aggcgaaagc
      241 ccaggacggc agcacggcga aagaagcgaa atggagcctg ctgaaacagg ttggcgccga
      301 cttcacgcca accgcttcgg aggcggcgag cgccaacttc tcctacacgg tgaaacaggc
      361 cccgggcccc ttcgtcgaag tgaccgccaa attcacctcg accgcgggcg tcggcgaaga
      421 cagctggaaa cagcccacgg aagccctgct gccgacccgg ttcacgatca ccttctcggg
      481 ctcggcctcc tacgacgcat cggaagtcgg cgaagggggc ggacaggcgc agtggagcgg
      541 caacgcggat ctgcgcgaaa tgccgagccc ctacccgccg gggacgttcc cctacgaact
      601 ggcgatgtac aaactcaacg cgggctcgat cacctactcc tactcagggt ccttcggtaa
      661 atgcagcgcg accgcgtccg gttcgatcga cctcgccgaa cagcccgacc tcgtcggtgg
      721 ggttgtgctg acgatcttcg acaaggaccc acgcgaatac tcggtcttcc tcggcatgcc
      781 gctgaccgcg aaagccacgg tccccgggct gctcggcccc tgcgaggacc ccgaagacga
      841 aggcgcgatc gacttcttcc cggggattgg cgtgccgatg ctcgtcaacg ctcccttccc
      901 gggtgggccg gtgagcccgg actggagctt ctccggctcg ggctcgagcg atttcccggg
      961 tccggatcag agctggcagt ggagcctgat cccgagcttc ggcacctgag caggaagccc
     1021 ggcatcactg gtcaggatgc cggcaggtag gtgagcagga ccacgccgga gtcgaagcgc
     1081 tgtacgtcgt cgagctccat ccgcaccgtt ccgcggtcgc cgagcacgtg cacgttgccc
     1141 ttgccccaga cgagcgggtt caggtagacc tcgtacacgt cgaccaggcc ccgctcggcg
     1201 agggcgtagg cgaactcacc gctgccgtgc atgaacaggt cgccgtcgac ttcgtccttc
     1261 agcctcggga tcgagtcgcc gaggtcgccg tcgagaagcg tcgcgttcca ctcgagatcg
     1321 ccggacaggg tggtggagcc gacgtgcttc ggcatcgagt tgaccatgtc cgcccatctg
     1381 ccctgctggc tcggccagta ggccgccagg ccctcgtagg tcttccggcc cagcaccagg
     1441 ccggcggatc tcgcctggcg ctcgaacgag tactcgccgt ggtccccgtc cgcctgcacc
     1501 cactcgccga ccgggtcgat gacaccgtcg acggtggcct gggtcgttgc gatcagcctg
     1561 cccatcgtgt tcctccttcg gtcaggagct ccctttgagc ccttctaccc aaacgacgaa
     1621 cggggacgcg cgatttcgac atcttccgag ctatttctgg ttcagccact cctcgtaggt
     1681 ggggccggcg agcttggcga tcgggccggg gaggacggcg ccggcggggt agaggccttc
     1741 ggggtcgtcg aagaagtccc cggcctcgat ccgcaggtcg tccccgcgct gggcggcgag
     1801 cagttttgcc gcctcgagca ggttctcctc gcgggggccg gcgacttcgg cgatcgggtt
     1861 gccgatcggc gccgagtccg ccccgtcgac gaggtcggcg agcgcctcgg cgacgacttc
     1921 cgcggcgacc agctgggtgc gcatctgggg caggcgggcg gtgtcgccct cagttcccca
     1981 ctcgaccagt tcggcgacga actcgtggaa ctgggcggcg cgcaggacgc gggcggggag
     2041 gggaccgttg aagtgggcct gctcctgggc caccttggcg gcgttgtagc cggtgttgaa
     2101 atggtcggtg ccgatgatcg agacgacgag gatctcctcg accccgctcg cagcacccgc
     2161 ctcctgcagg ttgcgggccg cggtcgtgaa gaactcggtc gcttccgcct ggtcgggcga
     2221 gggcccggtg gcggcgtcga cgatcaggtc gactccctcc agcgcggctc ccaggccctc
     2281 gccggtgatc acgtcgacac cctcggagcg ggagatcggc accacctcgt gcccgcgctc
     2341 ccgcaggatc tcgacggtgg ggcggccgag gcggccggtc gcaccggcga cggcgatctt
     2401 cttctcagtt gccatggttg ctttcctttc agtccgaggg ccgcttctgg gtcggccgtt
     2461 tttccttcgt ctatgaggta gacggagcgg agggcagaga tgtgaccgag tcgggacaga
     2521 aagatctggc agcgcggttc gagcagaacc gcgatcgcct ccgggcggtc gccttccgca
     2581 tgctcggcgc cagcggcgaa gccgacgagg cggtgcagga ggcctggttg cggctcgacc
     2641 gcgccgaccc ggaggtgatc gaggacctgc cgagctggct gacgaccgtg gtcgcgcgga
     2701 tctgcctcga catgctgcgg gcccgacagg cccgccacga ggagccgctc ggcccgcacg
     2761 tgcccgatcc gatcgtcagc ccggccgcgg gcggcgaccc cgagcacgag gcgctggtcg
     2821 ccgactcggt cggcctggcg atgctcggcg tgctggagac cctgagcccg gaggaacgcg
     2881 tcgcctttgt cctccacgac accttcgcgg tccccttcga gcaggtcgcc gacctgctcg
     2941 accgctcgcc ggccgccgcg cgcaaggccg ccagccgcgc tcgccagcgg gtcgaggcag
     3001 aaccgaccga gcccgacgtc gacctcgagc gccagcgcga ggtcgtcgac gccttcttcg
     3061 ccgccgcccg cgagggcgac ttcgacgccc tggtcgccgt cctccacccc gaaatcgtgc
     3121 tgcgctccga cggtggtgag gcccgcccga cggccaacca cgtgatccgc ggcgccgccg
     3181 aggtcgccgg ccgcgcgctg accttcgcgc acctctcgcc ccacgtccgg ccggtgctcg
     3241 tcaacggcgt cgccggcgtc ctcgtcgccc ccgaaggcaa gccgttctcg gtgatggcct
     3301 tcaccgtcgc cggcggcagg gtggtcgcga tcgatgccct cgccgatccg gagcggctcg
     3361 cgcggctcga gttcggcctt gggacttagt gcgattatgc actacccctt agactccccg
     3421 ttgtggcggt gaagacagct cttcggcttc ggttcaacag accatcctgg gcgctgatcg
     3481 gcgcgacgct cgcgctggca atcgccgtgc tggtgccggc gagggcggac gcctttggca
     3541 ccttcgccaa cggtgtgctc acggtcagtg gcggcgaggg gaaaatcgtt cctcgttgcg
     3601 caagtgacgg cgagctcacc gtcagcggcg tttccgtcga cgacgggccc gtctactgcc
     3661 gcgacctgag gcgaatcgag gcttcctcgt ccatctccac tctcttcgac ttctcgcagc
     3721 tgcccgacga cctcggtggc ggccaggggc cgatcgagat ccacgccacc tccacgacga
     3781 gcggcaagct cgaatacgcc agcgacaagt tcatcggggc cccgggtcac atcaacgtct
     3841 tcgatggagg gctcgacttc gactccatcg tcggcggcaa cctcaacgac aggctgagtg
     3901 ggggcgcgga cagcgacaag attgaaggcg ggcgcggaaa cgatgttctc ctcggcggtt
     3961 ctggggcgga caagctgctc ggtgggcccg gccgcgacac cttgaaaggc ggcgccggga
     4021 gcgacaagct gctcggcggc cccggcaagg acaccgaaaa gcagtagcca gccgagaggt
     4081 ctcgctacgc tcggatcgtt gacgccggag gacctcgaga acctcgcgca cctgcgccgc
     4141 gcccgcgacc tgatcgaccg cgagtacgcg cggccgctcg acgttgacga gatcgcgcgg
     4201 gcggcgctga tgtcgacggc gcacttctcg cggcgcttcc gggcggccta cggggagacg
     4261 ccgtacagct acctgatgac ccgccggatc gagaaggcaa aggcgctgct gcgcggcggc
     4321 gagctgtcgg tgaccgaggt ctgcatggag gtcgggtgta cctcgctggg ctcgttcagc
     4381 gcccgcttca ccgagctggt cggggaaacg ccgacgacct accgggcccg cgaccacgga
     4441 cggctggcgg cgatcccgag ctgctttgcc aaggacatca cccgtccggc gcgcaagccg
     4501 agcaggaatg gagaaacgcg ggccgtgccg gcgccttagc cttggcgcca tgaacctcac
     4561 tctctccacc tgcttcgtcc aggtccaaga tcccgacagc gcgctggcct tctaccgcga
     4621 ccagctcggc ctcgaagttc gcaacgacgt cggcaaaggc gattaccgct ggatcaccgt
     4681 cggcggcgcc gaccagcccg acgtggcgat cgtcctcacc aactacgtga acggcggccc
     4741 cgacgacagc gacgcggtcg ccgcgctgct cgccaagggc gcgatgaacg gcgtccactt
     4801 ccactccgac gacctcgacg gcgtcttcgc caagctgcag gcggccgggg tcgaggtggt
     4861 cgaagagccg aaggagcagc cctggggcac gcgggactgc gcggtgcgcg acccgtctgg
     4921 caatctggtc cggatcgacc agccgccggc ctcgtgagca aagccaaaaa gggaaagagc
     4981 gcggcggcgc ccgccgccga cagccacgac ctgatccgcg tgcacggcgc gcgggtcaac
     5041 aacctcaagg acgtcagcgt cgagatcccg aagcgccggc tgaccgcgtt caccggcgtc
     5101 tccggctcgg gcaagagctc gctcgtcttc ggcacgatcg cggccgagtc ccagcggctg
     5161 atcaacgaga cctacagcgc cttcgtgcag ggcttcatgc cgaccctggc gcggccggag
     5221 gtcgacgtgc tcgaggggct gacgaccgcg atcacggtcg accaggagcg gatgggatcc
     5281 gacacccgct ccacggtcgg caccgtcacc gatgcgaacg cgatgctg
//
LOCUS       DASRIG010000210         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_106798, whole genome shotgun sequence.
ACCESSION   DASRIG010000210 DASRIG010000000
VERSION     DASRIG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106798"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFB52_05830"
     CDS             <1..262
                     /locus_tag="VFB52_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05886.1"
                     /translation="ENHELVVTVQDPDGGELGEGLTANFETGRPPGMPQGQEQRLVMS
                     IGATASFATPGPHAAVVIVNGEELGRARFYLMEGGGPEEELA"
     gene            complement(300..1268)
                     /locus_tag="VFB52_05835"
     CDS             complement(300..1268)
                     /locus_tag="VFB52_05835"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HZO05887.1"
                     /translation="METITSEQPTVPAEPESPHGNVDEHGNVVPRRSPLRAAIKTGRP
                     KEWIKNVFVFAGLLFSGAFDQSHEVLLSVLTFVSFCLISSAGYFVNDLIDVELDRKHP
                     KKRFRPIAAGELSEGTAKLIAPVLAIVAIALAFATVNWEVGLMVVGYGVAQMAYSLGL
                     KQIVIIDVMTLAGLFILRVAAGASAVDAHASEWLILCTGMLAAFLGFTKRRQEAVSEL
                     HEGTSTRPVLEHYSLPFLDQMVSLVTTGTVISYAIYTVNSPLIGSEMMFTIPPVIYGI
                     FRYLYLIYDRSDDRTMAQIVAGDRGIQAAGASYAIIAFLLLYVFNG"
     gene            complement(1347..1595)
                     /locus_tag="VFB52_05840"
     CDS             complement(1347..1595)
                     /locus_tag="VFB52_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05888.1"
                     /translation="MQTMRDKRTDERLDDLNAKVDRGFERVEAEIRALRAEMRAELRT
                     EMQSLRTEMNARFDAMNRTMLWFAGALVIAIVASNLAS"
     gene            complement(1680..3290)
                     /locus_tag="VFB52_05845"
     CDS             complement(1680..3290)
                     /locus_tag="VFB52_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO05889.1"
                     /translation="MAERRLKHWGWGYEDQTPTATQMREAAEGIRERFGFGGEVEKPV
                     PLEAVELRPARLKRPASLGDLFSDDRYERVSRALGKAYRDIVRGFRGQFENPPDLVAF
                     PREESEIETVLSWAEAEGAAVIPFGGGTSVCGGVEARLGERPVVTMDLRRLDRVLDVD
                     PASLAARIQAGATGPRLEEQLREHGLTLRHFPQSFEYATLGGWIATRAGGHFATLETH
                     IDDLVESVRAITPRGNWESRRLPGSGAGPSPDRLLIGSEGILGAISEAWVRVRPRPEL
                     KSSCGVEFTDFYAAARAVREIAQSGLHPANCRLLDASEAELTGAGSGEADLLILGFES
                     AQVPVGADMEAALEIARAHGGAPGEVRSSAAQAGSEDAVGAWRGAFLLAPYLRDTFVA
                     CGVLSETFETAITWDRLEEFHAEVMEATRAAVAEVSGVAAEGPGAPRVSCRFTHVYPD
                     GAAPYFTVLAPAIRGGEVEQWDEIKAAVSDALIDAGGTITHHHAVGRDHRPWYDRQRP
                     APFAAALRAAKAELDPAATLNPGVLIDP"
     gene            complement(3311..5299)
                     /locus_tag="VFB52_05850"
     CDS             complement(3311..5299)
                     /locus_tag="VFB52_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO05890.1"
                     /translation="MSYFVTGATGFIGRNLIERLLQREGTIYALVRAGSRGRLEELRT
                     SWGADGARIVPVSGDLTQAGLAVSEEDLVAMRGEIDHFFHLAAIYDITANAEAQEIAN
                     VEGTRHALELAGAVEAGCFHQVSSIAAAGLYRGEWREDMFEEAEKLDNNPYFRTKHES
                     ERLVRESARPWRVYRPGIVVGDSRTGEIDKIDGPYYFFKSLQRARKVLPSWLPIAGIE
                     GGEINIVPVDFVASAIDHIAHEPDLDGRAFHLTDPDPRTAGEVINIFAKAADAPQMAF
                     RAGTEITEPATGAVRGVLKYLPPAKRVVKVGFGQLGLPAETLSYIDYPTHFDSSETQR
                     VLEGTGIDVPPLESYASRVWDYWERNLDPDLFKDRSLSGAVRGKIVMITGASSGIGKA
                     TAVKVADAGATVLLIARSVEKLEETKAEIVAAGGTAHIHRCDMADIEDIERMAEEVLA
                     YHGHVDILVNNAGRSIRRSVALSYDRFHDYERTIQLNYLGALRLILALLPTMRARKSG
                     QIINISSIGVQTNPPRFSAYVASKAALDAFSRVIASEVKEDKVHLTTINMPLVRTPMI
                     APTRMYDMFPAITPEEAAEMIARAMVKKPKKVATKLGNFGELLYAVAPKASDSILSIA
                     YGLFPDSQAAKGKESEARDKAPSTEAVAFAHLMKGVHW"
     gene            5361..>6834
                     /locus_tag="VFB52_05855"
     CDS             5361..>6834
                     /locus_tag="VFB52_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZO05891.1"
                     /translation="MPETATVQIAMPEMGESVTEGIVLEWHVAEGDTVSVNDPVVTVS
                     TDKVDAEVPAPADGVITKLLAAVDDEVKVGAILAEMDATGEVDPDSPALAGNGAASAD
                     TALAPESSGEAPDAPGDTESSTEEPGGGETLTIAMPEMGESVTEGVVLEWHVAEGDAV
                     SEGDTVIEVSTDKVDAEVPAPATGVITKLLVNVDDVVHVGAPLVEMETGAGGGGAKAA
                     SPASSDGATAAAPAGNGSSDVRATPVARRVAAANGVDIGSVTGTGDGGKVMKGDVLAA
                     GDRGGNGAAPTAGEPKLLRGPAAMLAKAMDESRAVPTATSFRTIAVDTLDAKRKAINV
                     VLKERGQKVSFTHLVAWAIVQAVKDFPVMVRSFEERDGKPHAIEKAPINLGIAVDVER
                     KDGSHSLMVPAIKNSQGLDFSGFHSYYEELITKTRENKLTADDFLGTNISLTNPGGIG
                     TVASVPRLLAGQSAIIATGSIAYPPEWAHATPERLRQLGVS"
BASE COUNT         1054 a         2342 c         2433 g         1005 t
ORIGIN      
        1 cgagaaccac gagctcgtcg tcaccgtcca ggaccccgac ggcggcgagc tcggcgaggg
       61 cctgaccgcc aacttcgaga ccggccgccc ccccggcatg ccccagggcc aggagcagcg
      121 cctggtgatg tccatcggcg ccaccgcctc cttcgccacc cccggccccc acgccgccgt
      181 cgtcatcgtc aacggcgagg agctgggccg cgcccgcttc tacctgatgg agggcggcgg
      241 ccccgaagag gaactggcct gagggatgtc agtttgttcg ggcatcccga acaaactgac
      301 tagccgttga agacgtagag cagcaggaag gcgatgatcg cgtaggaggc gccggcggct
      361 tggatgccgc ggtcaccggc gacgatctgg gccatcgtgc gatcgtcgga gcggtcgtag
      421 atcaggtaga ggtagcggaa gatgccgtag atcacgggcg ggatcgtgaa catcatctcg
      481 ctgccgatca ggggcgagtt gacggtgtag atcgcgtagc tgatcacggt gccggtggtg
      541 accagggaga ccatctggtc gaggaagggc agtgagtagt gctcgaggac cggccgggtg
      601 gaggtgccct cgtgcagctc ggagaccgcc tcctggcggc gcttggtgaa gccgaggaag
      661 gcggcgagca tgccggtgca gaggatcagc cactcggagg cgtgggcgtc gacggcgctg
      721 gcgccggcgg cgacgcggag gatgaacaga cccgcgaggg tcatcacgtc gatgatcacg
      781 atctgcttga ggccgaggct gtacgccatc tgggcgacgc cgtagccgac caccatcagg
      841 ccgacctccc agttgaccgt ggcgaaggcg agggcgatcg cgacgatcgc gagcacgggc
      901 gcgatcagtt tcgcggtccc ctccgagagc tcgcccgcgg cgatcggccg gaagcgcttc
      961 ttcgggtgct tgcggtcgag ctcgacgtcg atcaggtcgt tgacgaagta gccggcgctg
     1021 gagatcaggc agaaggagac gaaggtgagg accgagagca ggacctcgtg cgactggtcg
     1081 aacgcgcccg agaagagcaa gccggcgaag acgaagacgt ttttgatcca ctccttcggc
     1141 cgcccggtct tgatcgccgc gcgcagcggc gagcgccgcg gcacgacgtt gccgtgctcg
     1201 tcgacgttgc cgtgcgggga ctcgggctca gccggcacgg tcggttgttc ggaggtgatc
     1261 gtttccatcg atcgagacgc gacagcgtac cgccggcacc cgtcaaccca ccctctgagg
     1321 ggatgcggcg aaacgcgccg ccccgctcag gaggcgaggt tcgaggcgac gatcgcgatc
     1381 acgagggcgc cggcaaacca gagcatcgtg cggttcatcg catcgaaacg cgcgttcatt
     1441 tccgttctca gggactgcat ctcggtacgc aactccgccc gcatttccgc ccgcagggcg
     1501 cgaatctcgg cctcgacccg ctcgaagccg cggtcgacct tcgcgttgag gtcgtcgagg
     1561 cgctcgtcgg tccgcttgtc ccgcatcgtc tgcatgcttg caccgtaagg aattgtctgc
     1621 aacggaacaa ggcgcaaacg tgccgattcg gcgcctgctt tgcagcaatt cacgcacgct
     1681 caggggtcga tcaggacgcc ggggttcagc gtcgccgccg ggtcgagctc ggccttggcg
     1741 gcgcgcagag cggcggcgaa gggtgccggg cgctggcggt cgtaccaggg cctgtggtcg
     1801 cggccgaccg cgtggtggtg ggtgatcgtg ccgccggcgt cgatcagggc gtcggagacg
     1861 gcggccttga tctcgtccca ctgctcgacc tcgccgccgc ggatcgccgg ggcgagcacc
     1921 gtgaagtagg gagcggcacc gtcgggatag acgtgggtga agcggcagct gacccgcggc
     1981 gcaccgggtc cctcggcggc gacgccggag acctcggcga cggcggcgcg ggtcgcctcc
     2041 atcacctcgg cgtggaactc ctcgaggcgg tcccaggtga tcgcggtctc gaaggtctcc
     2101 gagaggaccc cgcaagcgac gaaggtgtcg cgcaggtagg gcgccagcag gaaggcgccg
     2161 cgccaggcgc cgaccgcgtc ctcggacccc gcctgagccg cggacgagcg cacctcgccg
     2221 ggcgcgccgc cgtgggcgcg ggcgatctcc agcgccgcct ccatgtcggc cccgaccggg
     2281 acctgggcgg actcgaagcc gaggatcagc aggtcggcct cgccgctgcc ggcgccggtc
     2341 agctccgcct cggaggcgtc gagcaggcgg cagttggccg ggtgcaggcc cgactgggcg
     2401 atctcccgga ccgcgcgcgc ggcggcgtag aaatcggtga actcgacccc gcaggaggac
     2461 ttgagctcgg gccgcgggcg cacccgcacc caggcctcgg agatcgctcc gaggatgccc
     2521 tcgctgccga tcagcaggcg atcgggcgag ggcccggcgc cggagccggg cagccggcgg
     2581 ctctcccagt tgccgcgcgg ggtgatcgcc cgcaccgact cgaccaggtc gtcgatgtgg
     2641 gtctccaggg tcgcgaagtg gccgccggcc cgggtagcga tccagccgcc gagggtcgcg
     2701 tactcgaacg actgcgggaa gtggcgcagg gtgaggccgt gctcgcgcaa ctgctcctcc
     2761 agccgcgggc cggtggcgcc ggcctggatc cgcgccgcca gcgaggccgg gtcgacatcg
     2821 agcacgcggt cgaggcggcg caggtccatc gtcaccaccg ggcgctcgcc cagccgcgcc
     2881 tcgaccccgc cgcagacgct ggtgccgccg ccgaacggga tcaccgcagc gccctcggcc
     2941 tcggcccagc tcagcaccgt ctcgatctcc gactcctcgc gcgggaaggc gaccagatcc
     3001 ggcgggttct cgaactggcc gcggaagccg cggacgatgt cgcggtaggc cttgccaagc
     3061 gcccgcgaga cgcgctcgta gcggtcgtcg ctgaacagat cgccgagcga agccggccgc
     3121 ttcagccgcg ccggccgcag ctcgaccgcc tccagcggca ccggcttctc gacctcgccg
     3181 ccgaacccga agcgctcgcg gatcccttcc gccgcctcgc gcatctgcgt cgccgtcggc
     3241 gtctggtcct cgtaccccca cccccagtgc ttgagcctcc gctcagccat gcgggcaacc
     3301 tacacgcgaa ctaccagtgc acgcctttca tcaggtgcgc gaaggcgacg gcctcggtgg
     3361 agggggcctt gtcgcgggcc tcgctctctt tgcctttcgc cgcctggctg tcggggaaga
     3421 ggccgtaggc gatgctgagg atcgagtcgc tggctttcgg cgcgaccgcg tagagcagct
     3481 cgccgaagtt gccgagtttg gtcgccacct tcttcggctt cttgaccatc gccctggcga
     3541 tcatctcggc ggcctcctcg ggggtgattg ccgggaacat gtcgtacatc cgggtcggcg
     3601 cgatcatcgg cgtccgcacc agcggcatgt tgatcgtggt caggtggacc ttgtcttctt
     3661 tgacctcgga ggcgatcacg cggctgaagg catcgagcgc ggccttcgag gcgacgtagg
     3721 ccgagaagcg gggcgggttg gtctgcaccc cgatcgagct gatgttgatg atctgacccg
     3781 acttgcgcgc ccgcatcgtc ggcaacagcg ccaggatcag gcgcagggcg ccgaggtagt
     3841 tgagctggat cgtgcgctcg tagtcgtgga agcggtcgta ggagagcgct accgagcggc
     3901 ggatcgagcg gccggcgttg ttgacgagga tgtcgacgtg gccgtggtag gcgagcacct
     3961 cttcggccat ccgctcgatg tcctcgatgt cggccatgtc gcagcggtgg atgtgggcgg
     4021 tgccgccggc ggcgacgatc tccgctttcg tctcctccag cttctcgacg ctgcgggcga
     4081 tcagcagcac ggttgcgccg gcgtcggcga ccttgaccgc ggtcgctttg ccgatccccg
     4141 acgaggcccc ggtgatcatc acgatcttgc cccgcaccgc gcccgagagc gagcggtcct
     4201 tgaacaggtc ggggtcgagg ttgcgctccc agtagtccca gacccggctc gcgtagctct
     4261 ccagcggcgg cacgtcgatc ccggtcccct ccagcacccg ctgggtctcg ctggagtcga
     4321 agtgggtcgg gtagtcgatg taggagaggg tctcggccgg cagcccgagc tggccgaacc
     4381 cgaccttgac gacccgcttg gccggcggca ggtacttgag cacgccgcgc acggcgccgg
     4441 tcgccggctc ggtgatctcg gtgcctgccc ggaacgccat ctgcggcgcg tcggcggcct
     4501 tggcgaagat gttgatcacc tcgcccgcgg tgcgcgggtc gggatcggtg agatggaagg
     4561 cgcggccgtc gaggtcgggc tcgtgggcga tgtggtcgat cgccgaggcg acgaagtcga
     4621 ccgggacgat gttgatctcg ccgccttcga tccccgcgat cggcagccag gacggcagca
     4681 ctttgcgcgc ccgctgcagc gacttgaaga agtaataggg cccgtcgatc ttgtcgatct
     4741 cgccggtgcg cgagtcgccg accacgatcc ccggccggta gacccgccag ggccgggccg
     4801 attcacggac cagccgctcg gactcgtgct tggtccggaa gtaggggttg ttgtcgagct
     4861 tttcggcctc ctcgaacatg tcctcgcgcc attcgccgcg gtagaggccg gcggcggcga
     4921 tcgagctgac ctggtggaag cagcccgcct cgaccgcgcc cgcgagctcg agcgcgtgcc
     4981 gggtgccctc gacgttggcg atctcctgcg cttccgcgtt ggcggtgatg tcgtagatcg
     5041 cggcgaggtg gaagaagtgg tcgatctcgc cccgcatcgc gaccaggtcc tcctcggaga
     5101 ccgccaggcc ggcctgcgtg aggtcgccgg agacgggcac gatgcgggct ccgtcggcgc
     5161 cccagctggt gcgcagctcc tccaggcggc cccgcgaccc ggcgcgtacc aaagcgtaga
     5221 tcgtgccctc gcgctgcagc agtcgctcga tcaggttgcg gccgatgaat ccggtggcgc
     5281 cggtgacgaa gtagctcatc gggtaccagg gtatctcgac ttcgtgccga cacccccttt
     5341 ttcataaact cagtcgagaa atgccagaga cggccaccgt gcaaatcgca atgcccgaga
     5401 tgggcgagtc ggtgaccgag ggcatcgtcc tggagtggca cgtcgccgag ggggacaccg
     5461 tctccgtcaa cgaccccgtg gtcaccgtct ccaccgacaa ggtcgacgcc gaagtcccgg
     5521 cgccggcgga cggcgtgatc accaagctgc tggccgcggt cgacgacgaa gtcaaggtcg
     5581 gcgcgatcct ggcggagatg gacgcgaccg gcgaggtcga ccccgactcc cccgccctgg
     5641 ccggcaacgg cgccgcctcg gcggacacgg cactggcacc cgagtcctcc ggcgaagccc
     5701 ccgacgcccc cggcgacacc gagagctcca ccgaggagcc cggcggcggc gagacgctga
     5761 cgatcgcgat gccggagatg ggcgagtcgg tgaccgaggg cgtggtgctc gagtggcacg
     5821 tggccgaggg cgacgcggtc tccgagggcg acacggtgat cgaggtctcg accgacaagg
     5881 tcgacgccga ggttcccgcc cccgccaccg gcgtgatcac caagctgctg gtcaacgtcg
     5941 acgacgtcgt ccacgtcggc gcccctctcg tggagatgga aacgggcgcc ggcggaggtg
     6001 gcgcgaaggc cgcgagcccg gcgtcttctg acggcgccac ggccgccgct cccgccggga
     6061 acggcagctc cgacgtgcgc gccaccccgg tggcccgccg cgtcgccgcc gccaacggcg
     6121 tcgacatcgg atcggtcacc gggaccggcg acggcggcaa ggtgatgaag ggcgacgtgc
     6181 tcgccgccgg cgaccgtggc ggcaacggcg ccgcgcccac cgcgggtgag ccgaaactgc
     6241 tgcgcggccc cgccgcgatg ctggccaaag cgatggacga gagccgcgcg gtcccgaccg
     6301 cgacctcgtt ccggacgatc gccgtcgaca ccctcgacgc caaacgcaag gcgatcaacg
     6361 tggtgctcaa agagcgcggc cagaaggtct ccttcaccca cctggtcgcc tgggcgatcg
     6421 tgcaggcggt caaggacttc ccggtcatgg tccgctcctt cgaagagcgc gacggcaaac
     6481 cgcacgcgat cgaaaaggcg ccgatcaacc tcgggatcgc ggtcgacgtc gagcgcaaag
     6541 acggctccca cagcctgatg gtgccggcga tcaaaaactc gcagggactc gacttctccg
     6601 gcttccactc ctactacgag gagctgatca ccaagacccg cgagaacaag ctgaccgcgg
     6661 acgacttcct cggcacgaac atctcgctga ccaaccccgg cgggatcggc acggtcgcct
     6721 cggtgccgcg cctgctcgcc ggccagagcg cgatcatcgc caccggatcg atcgcctacc
     6781 cgcccgagtg ggcccacgcg accccggagc ggctgcgcca gctcggcgtc tcga
//
LOCUS       DASRIG010000211         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1158903, whole genome shotgun sequence.
ACCESSION   DASRIG010000211 DASRIG010000000
VERSION     DASRIG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158903"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFB52_05860"
     CDS             complement(<1..531)
                     /locus_tag="VFB52_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO05892.1"
                     /translation="MSPALLSAQELTKSYGELDVLGRVTLEVAPGEICCLVGPTGCGK
                     STLLRLLLALEQPDAGTLTVGASGAEVGVVFQEDALMPWRSIADNVALPLELAGVAAA
                     ERRERALASLGHVGLDGRADQLPGELSGGMRQYVSLARAMVGEPRLLVLDEPFGHFDP
                     ESRVAVESLLLEWLAER"
     gene            complement(528..1268)
                     /locus_tag="VFB52_05865"
     CDS             complement(528..1268)
                     /locus_tag="VFB52_05865"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05893.1"
                     /translation="MERLEAAGVGFGYGEEPVVDSFGLVVEPGEMLCLVGRTGVGKTT
                     VAKLLAGILAPDEGEVSFGGRAVAGLRDVVSFVFQQGSLLPWRSVADNVALALEIDRV
                     PKAERRRQVEELLAKVGLSGYEDFLPAQISGGMRQRAAVARAFASPAPILIMDEPFGA
                     LDAQTRIRLEEELLRLWEEDRRTIVFLTNDHDEALVLGDRIVGIGGPPARIGGTLRVP
                     LSRPRSNLDDEVVALRSDVRELLTAVPA"
     gene            complement(1268..>2065)
                     /locus_tag="VFB52_05870"
     CDS             complement(1268..>2065)
                     /locus_tag="VFB52_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO05894.1"
                     /translation="APAAPGRRRRNGQRSRGSLVVKVGAVALTFAAFLLFLQYGLVHL
                     GVETYIVPKPSTVVSSLNDFWATDLRDALWFTAREVVFGLLLGVLVGILLAILIDALP
                     LLEYVILPYVIALVMTPMIALVPVVFLQFGDTLLVRICVVAFAAAPMMMLNTLTGLRR
                     TPEIRLDVMRALRASRVQTLWKVKLPSALPMMFTGLLIGSIFAVIAGVGTEFVSSNQG
                     LGAKIVYYSSVAQTDVVYAAILLLVVMGLVLFGTIKLLERRFAGWRD"
BASE COUNT          336 a          749 c          732 g          248 t
ORIGIN      
        1 gcgctcggcg agccactcga gcagcaggga ctcgaccgcc acgcgggact cggggtcgaa
       61 gtggccgaac ggctcgtcga gcacgagcag gcgcggctcg ccgaccatcg cccgcgccag
      121 cgagacgtac tggcgcatgc cgccggagag ctcgccgggc agctggtcgg cgcggccgtc
      181 gaggccgacg tggccgagcg aggcgagcgc ccgctcgcgg cgctcggcgg cggcgacccc
      241 ggccagctcg agcggcaggg cgacgttgtc ggcgatcgag cgccagggca tcagcgcgtc
      301 ttcctggaag acgacgccga cctcggcgcc gctggccccg acggtgagcg tcccggcatc
      361 cggctgctcg agcgcgagca gcagccgcaa cagggtcgac ttgccgcacc cggtcggccc
      421 gaccaggcag cagatctcgc cgggcgccac ctcgagggtg accctcccga gcacgtcgag
      481 ctcgccgtag gacttggtca gctcctgggc gctgagcagg gccgggctca cgcgggcacc
      541 gccgtcagca gctcacggac atctgagcgc agggccacca cctcgtcgtc gaggttggag
      601 cgcggccgcg agagcggcac ccgcagggtg cccccgatcc gggccggcgg tccgccgatc
      661 ccgacgatcc ggtcgccgag aaccagcgcc tcgtcgtggt cgttggtgag gaagacgatc
      721 gtgcgccggt cctcttccca gaggcgcagc agctcctcct cgagccggat ccgggtctgc
      781 gcgtcgagcg ccccgaacgg ctcgtccatg atcaggatcg gcgccgggct ggcgaaggcg
      841 cgggcgaccg cggcccgctg gcgcatcccg ccggagatct gggccgggag gaagtcctcg
      901 tacccgctca gcccgacctt ggccagcagc tcctcgacct gccgccggcg ctcggccttc
      961 ggcacgcggt cgatctccag cgccagggcg acgttgtcgg cgaccgagcg ccagggcagc
     1021 agggagccct gctggaagac gaaggagacg acgtcgcgca gccccgccac ggcgcggccg
     1081 ccgaagctga cctcgccttc gtcgggggcg aggatcccgg ccagcagctt cgcgaccgtc
     1141 gtcttgccga ccccggtgcg gccgaccagg cagagcatct cgccgggctc gaccaccagc
     1201 ccgaagctgt cgaccaccgg ctcctcgccg tagccgaagc cgacgcctgc cgcctccagc
     1261 cgctccatca gtccctccag ccggcgaagc gccgttcgag tagtttgatc gtcccgaaca
     1321 ggaccaggcc catgaccacg agcagcagga tcgccgcgta gacgacgtcg gtctgggcga
     1381 ccgaggagta gtagacgatt ttcgccccga gcccctggtt gctggagacg aactcggtgc
     1441 cgaccccggc gatcacagcg aagatcgagc cgatcagcag cccggtgaac atcatcggca
     1501 gggcgctcgg cagcttcacc ttccagagcg tctgcacgcg gctcgcccgc agcgcccgca
     1561 tcacgtcgag gcggatttcc ggcgttcgcc gcagcccggt gagcgtgttc agcatcatca
     1621 tcggcgccgc cgcgaaggcg accacgcaga tccgcaccag cagggtgtcg ccgaactgca
     1681 agaagaccac cggcaccagg gcgatcatcg gcgtcatcac cagggcgatc acgtagggca
     1741 ggatcacgta ctcgagcagc ggcagagcgt cgatcaggat cgccagcagg atcccgacga
     1801 ggacgccgag cagcagcccg aagaccacct cgcgggcggt gaaccagagc gcgtcgcgca
     1861 ggtcggtcgc ccagaagtcg ttcagcgagg agaccacggt gctcggcttc ggcacgatgt
     1921 aggtctcgac cccgaggtgg acgagcccgt actggaggaa gagcaggaag gcggcgaagg
     1981 tgagggcgac ggcccccacc ttgacgacca gggagcctcg gctccgctgg ccgtttcgcc
     2041 gccgccgacc gggggcggcg ggcgc
//
LOCUS       DASRIG010000212         9849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3771127, whole genome shotgun sequence.
ACCESSION   DASRIG010000212 DASRIG010000000
VERSION     DASRIG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771127"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFB52_05875"
     CDS             <1..465
                     /locus_tag="VFB52_05875"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZO05895.1"
                     /translation="KPLTTDAMVSHVDFLPTLASLAGTPKAARSNWQGKDYSQLVLQP
                     KKSKTVQDYVVFTYDDVQSGQKSGPYPKAPNHIVSIREERWKLAKYYAVEPKKPTPPQ
                     WEMYDLKEDPEETENLAYKHYKRSPLQEREYQRLKKKLAAVEKTRLKSPKTS"
     gene            complement(469..1845)
                     /locus_tag="VFB52_05880"
     CDS             complement(469..1845)
                     /locus_tag="VFB52_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO05896.1"
                     /translation="MPDLPPTADRRTLNRRDLLAGALAGGAAAALAGSPAAALAASPG
                     DASKTQPLPAVTFFKEPGLNFQMLFALGGAGYGVSEVGEVLATFDRIHGKGDTYLAAF
                     EEFQRLGRQLRQRADRDRDAGRLESARSSYLRAAMYLDQALFFTLASKNPSRKHEGAM
                     YREMEGAWASAGALFKPRFEPVSIRWGGGKLPGWFLSPGGKKRRPTVIINTGSDAQNI
                     DMYVYGGAAALERGWNALIFEGPGQGANLFLRNQAFIPEWERVITPVVDFLRAHPKVD
                     KRRICLVGQSFGGYLVARAAAFEHRLAAVVPDPGVVDVFSDWEKSLPPVMIELLNAGR
                     RNEFNGYWDAIPKEVSVDEAAGLAKRAEIYGNGSGYEKMKLAQKFVLGKKVARRITAP
                     VGILSPELEQFFPGQPETLAKWLKAPHKLIKFTVAEGGEYHCEPMAPQLRNERVFDWL
                     ETNLRPVR"
     gene            complement(1889..2893)
                     /locus_tag="VFB52_05885"
     CDS             complement(1889..2893)
                     /locus_tag="VFB52_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05897.1"
                     /translation="MLARSSSRILGLALVALIVPLFAAAPARAASASCEVTDAFPQGD
                     PAGDILQVNDSSNSVTHIYRDGDEIVVSSNADRNPAVCTGAVPTVFNIDRIIYKTRRS
                     VPFINYHGDGPLAPGVSPEPGAAEIEITIQEDYDPKVVNIAGTNESERIVAGQIHREL
                     SGVNMNADADKDADVIFDAPERAFLRIIARDGDDELSALGGPGFAGPFEPAERLTFAG
                     GLGDDVLSGGPFRDVLSGNEGDDELFGGRGRDKLTIGPGRDLAEGGKGNDDIYNQSDV
                     GGIEPDLFPDRVFGGAGDDSISVGQQLRGDRVECGAGRDDVFKDAGDITLDCEETGFR
                     "
     gene            2960..3616
                     /locus_tag="VFB52_05890"
     CDS             2960..3616
                     /locus_tag="VFB52_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO05898.1"
                     /translation="MGVTVDEITVGDAPAAWTALGFALDGDTLALGPVRLRAAGPEAG
                     KGLVGWSLRGLGESELDEIDGLPTAYSERPLPDEPPAHPNGVTGLDHVVAITPRLERT
                     IAALEAVGLDLRRIREEPTPAGAPRQAFFRLGPTILEVVQEPADVAAQNGPDRPAFFW
                     GIALLAPDLEATVAALGEDRVSEIRTAIQPGRQIATLRRAAGLSLPVALMTPDAGRAP
                     "
     gene            complement(3585..4070)
                     /locus_tag="VFB52_05895"
     CDS             complement(3585..4070)
                     /locus_tag="VFB52_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZO05899.1"
                     /translation="MSRIATIGVYGFDSESFLAALAAAEIELLLDVRQRRGVRGSEYA
                     WANAGRLQEALAGAGIAYTHLKELAPTTELREAQYREDDRRGEGKRSRTELAPEYARR
                     YSEEILDPLDLGPIVNWIGASQAALLCVERDPEACHRSLIAERLRQDWGFRVLDLRPE
                     S"
     gene            4101..5423
                     /locus_tag="VFB52_05900"
     CDS             4101..5423
                     /locus_tag="VFB52_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZO05900.1"
                     /translation="MDTPTKYAVSEDGVNIAYQAHGEGPLDLVFVPGFVSHVELAWED
                     PALARFLRRLASFSRLIFFDKRGQGLSDRLGRPPTLEESMDDLRAVMDAADCKRPALF
                     GISEGGPMSILFAATYPERVAALVLYGTYARMLRSEDFPAGVNPERYEGWAELVRDEW
                     GGPVAVDLWAPEEVGNPEFESWWARLLRQGTSPAGALELMELYREIDVRPVLPAIDVP
                     TLVLHRLGDRLIPPRQAEYLAANIPGARYVELPGESHLPTGGDQEQLLDEVEEFLVGS
                     RVSEPERALATVLFTDIVGSTETAARIGDRRWRDLIERHDQTVRRELAVHRGTEVKTM
                     GDGFLATFDGPARAIRCAVEIRDELARIGVEVRAGIHTGEVDLIGTDVAGMAVNIGAR
                     VGALAGSGEVLVSSTVRELVVGSGIEFEERGSHELKGAPGDWRLFAVR"
     gene            5431..5793
                     /locus_tag="VFB52_05905"
     CDS             5431..5793
                     /locus_tag="VFB52_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05901.1"
                     /translation="MPDRPPPPPPPPPGAGPPPPPPPPPPPPPAPPAPPPAPPAPPPP
                     DQRVGDEKLTIFGWTRKQVLEGSKRWLQTCVIVVLVGLLLGNKSRLAGAILIGIAVLG
                     VIFVYVLRDFVWSKDDDA"
     gene            5786..5935
                     /locus_tag="VFB52_05910"
     CDS             5786..5935
                     /locus_tag="VFB52_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05902.1"
                     /translation="MPNTLAAWTSMALLISLIVLAVISTHWLPLVLVAVIAFGYLAYE
                     YLSKH"
     gene            5939..6406
                     /locus_tag="VFB52_05915"
     CDS             5939..6406
                     /locus_tag="VFB52_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05903.1"
                     /translation="MRLRWQIPLVVLAAAGVLVAAVLIADGCSQRLVESDTSAESQAP
                     LIERSERERPEPETVEPSHCPPDVEGCAVTEGRIAYIEAVDPDGDGDAHFVVVDGASI
                     TLPGLTAIDVEKSLRPHPLPGVGDLISAAGPVQTGSYGQEQIHALEFHVAEGK"
     gene            6495..8009
                     /locus_tag="VFB52_05920"
     CDS             6495..8009
                     /locus_tag="VFB52_05920"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZO05904.1"
                     /translation="MLAASALGREAWVANYEGQGVSVIDVATNTVSATIPTGPAAGPY
                     NLAISPDGKTAYVLNYDGASLQPIDVATKALLGPPIQVGEAPIGIAITPDGKRAVVAS
                     TQEKDIFFVDLQARQLIGSPLEVGSSPQGVAITPDGSRAYVANNDGESVAVVDLATNQ
                     LLGLPIPVGDGPYGLAVTPDGKYVVVASADDKVVTTISTATNQVVGVPVPADDPQTVA
                     ISPDGTRAFVTNYEGSSITVLGVPALNLLGSIPIAATEYIALTPDGKRGFASQYYGPS
                     VAAFDPFAMQPFGAPVEVGKGAGQVAVVPDQSPSAAYTAPARVRPGVPATLNAAGSSD
                     SDGTVAGFAWNFGDGKTASATARKQKHTFAKPGKFKVALTVTDNEGCSAPLVFTGQTA
                     YCSGSPGATVTKTVKVAYPTTKLKCPASAKPGGCKFSLQAVAWKGKGKKRTLKAQSAV
                     AVAKVKAGKSKDLALKPKKAFAKKLAGAKKILVREKRTIAGETRTRVTKLKIVG"
     gene            complement(8014..8772)
                     /locus_tag="VFB52_05925"
     CDS             complement(8014..8772)
                     /locus_tag="VFB52_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05905.1"
                     /translation="MTLLGRRLNPCLLVAAVIGLAFAVVLIGSGGAGASAPAKPTGYW
                     KLFVKLEVTSTGTATNPRCYPDPEQVAPSPVSGTATQKIVWKTTRPTTVEFHETPGGI
                     PVAGETNYTPAFRGKLTETRSGDLDLGGEPRGCYGSSQPPKTDCGSRSALGGIYVNPL
                     GGIRSWKGFRIEPEQTPGERWQSCGLAGAQFKLDPLDLEITASRGKLTGKAPKLVFSK
                     TKQLKAADTNEGVKSSATGTLKYTVTLIRASSSS"
     gene            complement(8802..9485)
                     /locus_tag="VFB52_05930"
     CDS             complement(8802..9485)
                     /locus_tag="VFB52_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZO05906.1"
                     /translation="MPTVLLVRHGQASFGAEDYDVLSPTGVRQSEVLAAALKRRGIAP
                     SHLVCGSLRRQSETALPFAEYGEAVIDPRWDEYDANEILTHHAETNVRLDATGGHAAD
                     GPPELTSKGFQAVIEPALAGWIEAGHESPSQNTWPLFAAAGSAALDDLKGALGRGETA
                     LVFTSGGTIAALCAALLEAPGAFLALNRVLANTGVSKLTIGASGTSLVSINDHSHLEE
                     VDRALITYR"
     gene            9580..>9849
                     /locus_tag="VFB52_05935"
     CDS             9580..>9849
                     /locus_tag="VFB52_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05907.1"
                     /translation="MRRTHFTDEHRLFRESVAEFCRREVTPNIERWREQHRIDREVWL
                     EAGRQDFIGLSMPPELGGTGLADFRFNQVLGEELAATSVAVNSALM"
BASE COUNT         1431 a         3450 c         3481 g         1487 t
ORIGIN      
        1 aaaccgctga ccaccgacgc gatggtctcc cacgtcgact tcctgccgac cctggccagc
       61 ctcgccggga cgccgaaggc ggcgcgctcg aactggcagg gcaaggacta ctcgcagctg
      121 gtcctgcagc cgaagaagag caaaacggtg caggactacg tcgtcttcac ctacgacgac
      181 gtccagtccg ggcagaagag tgggccctac ccgaaagccc cgaaccacat cgtctcgatc
      241 cgcgaagagc gctggaagct ggcgaagtac tacgcggtcg aaccgaaaaa accgacgccg
      301 ccgcagtggg agatgtacga cctcaaggaa gatccggaag agacggaaaa ccttgcgtac
      361 aagcactaca aacgctcgcc cctgcaggag cgcgaatacc agcggctgaa aaagaagctg
      421 gccgcggtcg agaagacccg gctgaagtcg ccgaaaacgt cctgagcttc agcgcacggg
      481 gcgcaggttc gtctcgagcc agtcgaagac gcgttcgttg cgcagctgcg gggccatcgg
      541 ctcgcagtgg tactcgccgc cctcggcgac cgtgaacttg atcagtttgt gcggggcctt
      601 cagccatttg gcgagcgtct ccggctggcc ggggaagaac tgctcgagct ccggcgagag
      661 gatccccacc ggtgcggtga tccgccgggc gaccttcttg ccgagcacga acttctgcgc
      721 cagtttcatc ttctcgtagc cgctgccgtt gccgtagatc tcggcgcgct tggccaggcc
      781 ggcggcctcg tcgacgctca cctctttggg gatcgcgtcc cagtagccgt tgaactcgtt
      841 gcggcggccg gcgttcagca gttcgatcat caccggcggc aggctctttt cccagtcgga
      901 gaagacatcg acgacgccgg ggtcggggac gaccgcggcg agccggtgct cgaaggcggc
      961 ggcgcgggcg accaggtagc cgccgaagct ctggccgacc aggcagatgc ggcgtttgtc
     1021 gaccttcggg tgggcgcgga ggaagtcgac cacgggcgtg atcacccgct cccactcggg
     1081 aatgaaggcc tggttgcgga ggaagaggtt ggcgccctgg ccgggccctt cgaagatcag
     1141 cgcgttccag ccgcgttcga gcgccgccgc gccgccgtag acgtacatgt cgatgttctg
     1201 cgcgtcggag ccggtgttga tgatcaccgt tggccggcgt ttttttccgc ccgggctgag
     1261 gaaccagccg ggcagcttgc cgccgcccca gcggatcgag accggctcga agcgcggctt
     1321 gaacagcgcc ccggcgctcg cccaggcgcc ctccatctcg cggtacatcg cgccctcatg
     1381 tttgcggctc gggttcttcg aagccaaggt gaagaacagc gcctggtcga ggtacatcgc
     1441 cgcgcgcagg tagctgctgc gggcgctctc caggcggccg gcgtcgcggt cgcggtcggc
     1501 gcgctggcgc agctggcggc cgagccgctg gaactcctcg aaggcggcga ggtaggtgtc
     1561 gcccttgccg tggatgcggt cgaaggtcgc cagcacctcg ccgacctcgg agacgccgta
     1621 gccggcgccg ccgagcgcga acagcatctg gaagttgagc cccggttctt tgaagaaggt
     1681 gaccgcgggc agcggctgcg tcttcgaagc gtcgccgggc gaggccgcca gcgccgcggc
     1741 gggcgagccc gccagcgccg cggccgctcc gccggcgagc gcgccggcga gcaggtcgcg
     1801 gcggttcagc gttcgccggt cagcggtggg gggcaggtcg ggcatgacga gcccggaatc
     1861 ctgtcgccgc cgtcggacga cagccggctc agcggaagcc ggtctcctcg cagtcgaggg
     1921 tgatgtcgcc ggcgtccttg aagacgtcgt cgcggccggc gccgcactcg actcggtcgc
     1981 cgcgcagctg ctggccgacg ctgatgctgt cgtcgccggc gccgccgaag acccggtcgg
     2041 ggaagaggtc cggttcgatc ccgccgacgt cgctctggtt gtagatgtcg tcgttgccct
     2101 tgccgccctc ggcgaggtcg cggccggggc cgatcgtgag tttgtcgcgg ccgcggccac
     2161 cgaacagctc gtcgtcgccc tcgttgccgc tgagcacgtc gcggaagggg ccgccgctga
     2221 gcacgtcgtc gccgagcccg ccggcgaaag tcagccgctc ggccggctcg aacggcccgg
     2281 caaagccggg gccgccgagg gcgctcagct cgtcgtcgcc gtccctggcg atgatccgca
     2341 ggaaggcccg ctcgggggcg tcgaagatca cgtcggcgtc tttgtcggcg tcggcgttca
     2401 tgttcacccc ggagagctcg cggtggatct gcccggccac gatccgctcg ctctcgttgg
     2461 tgccggcgat gttgaccacc ttcgggtcgt agtcctcctg gatcgtgatc tcgatctccg
     2521 cggcgcccgg ctcggggctg acgccgggcg ccagcggccc gtcgccgtgg tagttgatga
     2581 agggcacgct gcgcctggtc ttgtagatga tccggtcgat attgaagacg gtggggaccg
     2641 cgccggtgca gaccgcgggg ttgcggtcgg cgttgctgga aacgacgatc tcgtcgccgt
     2701 cgcggtagat gtgggtgacg ctgttggagc tgtcgttgac ctggaggatg tcgcccgccg
     2761 ggtcgccctg cgggaaggcg tcggtcacct cgcagctggc gctggcggcg cgggccggcg
     2821 cggcggcgaa cagcggcacg atcaaggcga cgagcgccag gccgaggatc cggctgctgg
     2881 agcgagcgag cacgcgattc agggtacctg cgtgccgcga cgccatctct gcggtggtcc
     2941 tgccggttac ggtgtgcggg tgggggtgac ggtcgacgag atcacggtgg gggacgcgcc
     3001 ggcggcctgg acggcgctcg gcttcgcgct cgacggcgac accctggcgc tcggcccggt
     3061 gcggctgcgc gccgccggcc cggaggccgg caagggcctg gtcgggtggt cgctgcgcgg
     3121 gctgggggag agcgagctgg acgagatcga cggcctgccg acggcgtact ccgagcgtcc
     3181 gctcccggac gagccaccgg cccaccccaa cggcgtcacc ggcctcgacc acgtggtcgc
     3241 gatcacgccc cggctggagc gcacgatcgc cgcactggag gcggtcggcc tcgacctgcg
     3301 ccggatccgc gaggagccga ccccggccgg ggcgccgcgc caggccttct tccgcctcgg
     3361 cccgacgatc ctcgaggtcg tccaagagcc cgccgacgtc gccgcccaaa acggccccga
     3421 ccgtcccgcc ttcttctggg gcatcgccct gctcgccccc gacctcgagg cgaccgtcgc
     3481 cgctctcggc gaggacaggg tcagcgagat ccggaccgcg atccagcccg gccgccagat
     3541 cgccaccctg cgccgcgccg ccggcctctc gctccccgtc gcgcttatga ctccggacgc
     3601 aggtcgagca ccctgaagcc ccagtcctgg cgcaggcgct cggcgatcaa ggagcggtgg
     3661 caggcctcgg ggtcgcgctc gacgcagagc agggccgcct ggctggcgcc gatccagttg
     3721 acgatcgggc cgaggtcgag cgggtcgagg atctcctcgc tgtagcggcg cgcgtactcg
     3781 ggcgccagct cggtgcggga gcgtttgccc tcgccgcggc ggtcgtcctc gcggtactgg
     3841 gcctcgcgca gctcggtggt cggcgccagc tccttcaggt gcgtgtaggc gatcccggcc
     3901 ccggccagcg cctcctgcag ccgcccggcg ttcgcccagg cgtactcgct gccgcgcacg
     3961 ccgcgccgct ggcgcacgtc gagcagcagc tcgatctcgg ccgcggccag cgcggcgagg
     4021 aacgactcgc tgtcgaaccc atagaccccg atcgtcgcga tcctgctcac caagccagtc
     4081 tgacgctaca ttcgcgggag atggacaccc ccaccaagta cgcggtctcc gaggacggcg
     4141 tcaacatcgc ctaccaggcg cacggcgaag gaccgctcga cctggtcttc gtgcccggct
     4201 tcgtctcgca cgtcgagctc gcctgggagg acccggcgct ggctcgcttc ctgcgccggc
     4261 tggcctcgtt ctcgcggctg atcttcttcg acaagcgcgg ccaggggctc tcggatcggc
     4321 tcgggcggcc gccgacgctg gaggagtcga tggacgacct gcgcgcggtg atggacgccg
     4381 ccgattgcaa gcggccggcc ctcttcggca tctccgaggg cgggccgatg tcgatcctct
     4441 tcgccgccac ctacccggag cgggtcgcgg cgctggtcct ctacgggacc tacgcgcgca
     4501 tgctgcgcag cgaggacttc ccggcgggcg tcaacccgga gcgctacgag ggctgggccg
     4561 agctggtccg cgacgaatgg ggtgggccgg tcgcggtcga cctctgggcg ccggaggagg
     4621 tcggcaaccc cgagtttgaa agctggtggg cgcggctgct gcgccagggg accagcccgg
     4681 ccggggcgct ggagctgatg gagctatacc gcgagatcga cgtgcgcccg gtgctgccgg
     4741 cgatcgacgt cccgaccctg gtcctgcacc gcctcggcga ccggctgatc ccgccgcgcc
     4801 aggccgagta cctcgccgcc aacatccccg gcgcccgcta cgtcgagctg cccggcgaaa
     4861 gccacctgcc gaccggcggc gaccaggagc agttgctcga cgaggtcgag gagttcctcg
     4921 tcggcagccg cgtctccgaa cccgaacggg cgctggcgac cgtgctcttc accgacatcg
     4981 tcggctcgac cgagaccgcg gcgcggatcg gcgaccgccg ctggcgcgac ctgatcgagc
     5041 gccacgacca gaccgtgcgc cgcgagctcg ccgtccaccg cggcaccgag gtgaagacga
     5101 tgggcgacgg cttcctcgcc accttcgacg gcccggcgcg ggcgatccgc tgcgcggtcg
     5161 agatccgcga cgagctggcc cggatcgggg tcgaggtgcg ggccggcatc cacaccggcg
     5221 aggtcgacct gatcggcacc gacgtggcgg gcatggcggt caacatcggc gcccgggtcg
     5281 gcgccctcgc cggcagcggc gaggtgctgg tctccagcac cgtgcgcgag ctggtcgtcg
     5341 gctcgggaat cgagttcgag gagcgcggca gccacgagct gaagggcgcc ccgggcgact
     5401 ggcgtctgtt tgctgttcga taattcaacg atgcccgacc ggccgccacc cccgccgccg
     5461 cccccgccgg gggccgggcc acccccgccc ccgcctcctc ctcctcctcc tcctccggcg
     5521 cctcccgctc cgccgccagc gcctcccgca ccgcccccgc cggatcagcg cgtcggcgac
     5581 gagaagctga cgatcttcgg ctggacccgc aaacaggtgc tggaggggtc gaagcgctgg
     5641 ctccagacct gcgtgatcgt ggtcctggtc gggctgctgc tcgggaacaa atcacggctg
     5701 gcgggtgcga tcctgatcgg catcgccgtg ctcggcgtga tcttcgtcta cgttctcagg
     5761 gacttcgtct ggtcgaagga cgacgatgcc taacacgctc gccgcctgga cctcgatggc
     5821 gctgctgatc agcctgatcg tgctcgcggt gatcagcacg cactggctgc cgctggtgct
     5881 ggtcgccgtg atcgcgttcg gctacctcgc ctacgaatac ctgtcgaagc actagacggt
     5941 gaggttgcgc tggcagatac ccctcgtcgt gctggcggct gccggcgtcc tggttgccgc
     6001 tgtcctgatc gcggacggct gctcccagcg gctggtcgaa tccgatacga gcgccgaaag
     6061 ccaggcgccg ctgatcgaac gcagcgagcg cgagcgaccc gagccggaaa cggtggagcc
     6121 ctcgcactgc ccgcctgacg tcgaaggctg cgcggtgacg gaaggccgga tcgcctacat
     6181 cgaggcggta gatcccgacg gcgacggcga cgcccacttc gtcgtcgtcg acggcgccag
     6241 catcaccctg cccggcctga ccgcgatcga cgtcgaaaag tccctgcgcc cgcacccgct
     6301 gccgggggtc ggcgacctga tcagcgccgc cggcccggtc cagaccggtt cctacggcca
     6361 ggaacagatc cacgcgctcg agttccacgt cgccgagggg aagtagagtg cccactgtga
     6421 atcgggggag cgaatcacga tcgtcgttgg tacgggcgct tgcgttggcc ggcctggcgc
     6481 tgctcgcttc gctggtgcta gccgcgtccg ctcttgggcg cgaagcctgg gtcgcgaact
     6541 acgaaggcca gggcgtctcg gtgatcgacg tcgcgaccaa cacggtgagc gcgacgatcc
     6601 cgaccggacc cgccgccggt ccttacaacc tggcgatctc acccgacggc aagaccgcct
     6661 acgtcctcaa ctacgacggg gcttccctgc agccgatcga cgtcgccacc aaggctctgc
     6721 tcgggccgcc gatccaggtc ggcgaagcgc cgatcgggat cgcgatcacc cccgacggca
     6781 aacgggcggt cgtcgccagc acccaggaaa aagacatctt cttcgtcgac ctgcaggccc
     6841 gccagctgat tgggagcccg ctcgaagtcg gctcctcgcc gcagggcgtt gcgatcacgc
     6901 ccgacggaag ccgcgcctac gtcgccaaca acgacggcga atcggtcgcc gtggtcgacc
     6961 tggcgacgaa ccagctgctt ggcctgccga tcccggtcgg tgacggtccc tacggtctcg
     7021 ccgtaacccc tgacggcaaa tacgtcgtgg tcgccagcgc cgacgacaag gtggtcacga
     7081 cgatttccac cgcgaccaac caggtcgtgg gtgtgccggt gccggcggac gacccgcaga
     7141 cggtcgcgat cagccccgac ggcacgcgtg ccttcgtcac caactacgaa ggcagctcga
     7201 tcacggtgct cggcgtcccc gcgctcaacc tgctcggttc gattccgatc gcagccaccg
     7261 aatacatcgc cctcaccccg gatggcaagc gcggcttcgc gagccagtac tacggacctt
     7321 ccgtcgccgc tttcgatccc ttcgcgatgc agccgttcgg cgccccggtc gaagtcggca
     7381 aaggtgccgg ccaggtcgcc gtcgtccccg accagtcgcc gtccgccgcc tacaccgcgc
     7441 cggcgcgggt gcggccaggg gtcccggcca ctctcaacgc cgccggctcg agcgactccg
     7501 acggcaccgt cgccggcttc gcctggaact tcggcgacgg caagaccgcg agcgccaccg
     7561 cgcgcaagca gaagcacacc ttcgccaagc cgggcaaatt caaagtcgcg ctcaccgtca
     7621 ccgacaacga aggctgctcg gccccgctcg tcttcaccgg ccagaccgcc tactgctcgg
     7681 gctcgccggg ggcgaccgtg accaagaccg tcaaggtcgc ctaccccacg acgaagctga
     7741 agtgcccggc cagcgccaaa cccggcggct gcaagttcag cctccaggcc gttgcctgga
     7801 agggcaaggg caaaaaacgg acgctgaagg cacagagcgc cgtcgccgtg gccaaggtca
     7861 aggccggcaa gtcgaaggac ctcgcgctga agccgaagaa ggcgttcgcg aagaagctgg
     7921 ccggggcgaa gaagatcctc gtgcgcgaaa agcgcacgat cgccggcgag acccgcaccc
     7981 gggtcaccaa gctcaagatc gtcggctagc cgcctagctg gagctgctgg cgcggatcag
     8041 cgtcacggtg tacttgagcg tgccggtcgc gctcgacttg acgccttcgt tggtgtccgc
     8101 cgccttcagc tgcttggtct tggagaagac gagcttcggc gccttgccgg tcagcttgcc
     8161 gcggctggcg gtgatctcga ggtcgagcgg gtcgagcttg aactgggcgc cggcgaggcc
     8221 gcagctttgc cagcgttcgc ccggggtctg ttcgggctcg atccggaagc ccttccagct
     8281 gcggatgccg cccagcgggt tgacgtagat gccgccgagg gccgagcgcg agccgcagtc
     8341 ggtcttgggc ggctggctgc tgccgtaaca gccgcgcggt tcgccgccga ggtcgaggtc
     8401 gccgctgcgc gtttcggtca gcttgccgcg gaaggcgggc gtgtagttgg tttccccggc
     8461 gaccgggatg ccgcccggcg tttcgtggaa ttcgaccgtg gtcggacggg tcgtcttcca
     8521 gacgattttc tgggtggcgg tgccgctgac cggcgagggc gcgacctgtt cggggtcggg
     8581 gtagcagcgc gggttggtcg ccgttcccgt gctcgtcact tcgagcttga cgaagagctt
     8641 ccagtacccg gtcggcttgg cgggagcgct cgcgcccgcg cccccggagc cgatcaagac
     8701 caccgcgaag gcgaggccga tgacggcggc gacgagcagg caggggttga ggcggcggcc
     8761 gagcagagtc acggtccggg aaaggtagcg accgccgcgc gctaccggta ggtgatcagc
     8821 gcccggtcga cctcttcgag gtgcgagtgg tcgttgatcg agaccagcga cgtgccggag
     8881 gcgccgatcg tcagcttgct gacgccggtg ttagcgagca cgcggttgag cgcgaggaag
     8941 gcgccgggcg cctcgagcaa cgccgcgcag agcgccgcga tcgtgccgcc gctggtgaag
     9001 acgagcgccg tctcgccgcg cccgagcgcg cccttcaggt cgtcgagggc ggcgctgccg
     9061 gcggcggcga agagcggcca ggtgttctgg ctcgggctct cgtggccggc ctcgatccag
     9121 cccgccagcg ccggctcgat taccgcctgg aagcccttgc tggtcagctc cggcgggccg
     9181 tccgcggcat gcccgcctgt cgcgtccagc cgcacgttgg tctcggcgtg gtgggtgagg
     9241 atctcgttgg cgtcgtactc gtcccagcgc gggtcgatga cggcctcgcc gtactccgcg
     9301 aacggcagcg ccgtttcgct ctggcgccgc agcgagccgc agaccaggtg gctgggggcg
     9361 atcccgcgcc gcttcagcgc cgccgccagg acctcggact ggcgcacacc ggtgggggag
     9421 agcacgtcgt agtcctcggc gccgaaggat gcttgtccgt ggcggacgag gaggacggtg
     9481 ggcacgcctt tgattatcga caatcgatcc gcgatcggca ctcgtcgctc ccgctagccc
     9541 ggagtaggtt ggcggcaatg gccacccggc gatcgacgca tgcgcagaac ccacttcact
     9601 gacgagcacc ggctctttcg cgagtcggtc gccgagttct gccgccgcga ggtgacgccg
     9661 aacatcgagc gctggcgcga gcagcaccgg atcgaccgcg aggtctggct cgaggccggg
     9721 cgccaggact tcatcgggct ctcgatgccg ccggagctgg ggggtaccgg gctcgccgac
     9781 ttccgcttca accaggtgct cggcgaggag ctggccgcca cctcggtcgc ggtcaactcg
     9841 gcgctgatg
//
LOCUS       DASRIG010000213         6731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2432976, whole genome shotgun sequence.
ACCESSION   DASRIG010000213 DASRIG010000000
VERSION     DASRIG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432976"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFB52_05940"
     CDS             <1..386
                     /locus_tag="VFB52_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO05908.1"
                     /translation="VSTDAEAMAALASSLDLIVDTVAAPHDLDPYLATLGREGALVLV
                     GIPPESEPHPSPSALGLISQRRTLTGSSIGGIAETQEMLDFCAEHGIVSEIEPIAINQ
                     INDAYERMLAGDVRFRFVIDMSTLS"
     gene            396..1766
                     /locus_tag="VFB52_05945"
     CDS             396..1766
                     /locus_tag="VFB52_05945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO05909.1"
                     /translation="MSRLRFNAFAMNCVSHIHHGQWTRPDTRQTEYASLDPWVELAQL
                     LERGKFDALFLADVIGTYDTYRGGRDTSVLEGLQIPVNDPSLIIPVMAHETENLGFAF
                     TQSVLQEQPFNFARRLSTLDHVTGGRIAWNVVTSYLENAGRNLGYGGLPPHEERYDRA
                     DEYLEVIYKLLEGSWDADAVVVDRERGVYADPAKIHNIDHAGRFYDVVGPHLSEPSAQ
                     RTPVIFQAGSSDRGREFAAHNAEAVFIIARNPEGARAQTEDMRARVVRHGREPGDIRF
                     FQALSVIVGGTEEEALRKQAEIDERASDEGYAAHMAGNIGVDLADIDLEAPLTELPER
                     YAGQSMMNVLIESAPDKSWAFGDLLRWIASVRVVGTPEQIADELGVYADAGVDGINLM
                     YYTTPGSFVDFIDGVAPVLQERGLMQSEYRPGTLREKLSDGADGPLISERHPAAGYRR
                     RQAVAA"
     gene            1763..2848
                     /locus_tag="VFB52_05950"
     CDS             1763..2848
                     /locus_tag="VFB52_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO05910.1"
                     /translation="MSRQRLIDTDVHERVELEQLLPHLPAIWHKYVTDYGWTPERFNP
                     YSQPTAGGLARADAVPEGGGLAGSSLPLLREQLLDEYGIEQAILTGWLSASALQEGWS
                     EFKTALMSAYNDHQVAHWLDREERLYGSVQVNAWDPEGAAREIERMAAHPKIVQVMLY
                     VGDQAYGSPRYHPIFAAAERHGLVVGIHHSENSTPALGRHRYYAEWHTLISQVFMSQA
                     VSLIFNGVFEKFPALKVILIEGGFSYVPHLMWRSDQQWRELRHEVPWVKRAPSRIIRE
                     QVRFASQPIEELTTPQLLSLIDQMGSEELICLSSDYPHWDFDSPFEALPPGFPEDLKQ
                     KIFWENALATYARLPAVVGEAGTRVPA"
     gene            2845..3216
                     /locus_tag="VFB52_05955"
     CDS             2845..3216
                     /locus_tag="VFB52_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZO05911.1"
                     /translation="MSQATATRARASLPAAELGPGEMTGLVVGEVAVVVVRTTEGELR
                     AYRDRCVHQGAPLSRGRLRAGTEGEEVGEYRLVEGQGVIKCPWHGYEYDAASGCALFD
                     SRRRLRRVTVEELDGEIVVSV"
     gene            complement(3221..4144)
                     /locus_tag="VFB52_05960"
     CDS             complement(3221..4144)
                     /locus_tag="VFB52_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO05912.1"
                     /translation="MRFEQLEYVTAVARLGSFRRAAEELHLSQPALSETVRKLEGELG
                     VEILDRGRSGVTISGRGRELLPHILEVIEAVDRLRGAADDQHHNSRVVRVGTVTAATA
                     PLLTPTIREFSEAHPSTQIEIVTSQQHWIHRDLLDGALDLGLVNYLGGDDMPPDLETT
                     ELLRGRPVVCVRADSPLASKALIGPEDLMGEPLVAMRTGYLMHRYVNRLLDGHTPKFS
                     YSADGAEMGKMMVAEGLGVAVLPSYSVVGDPLEQSGRITFREIDDDTDVMLTIQRRRS
                     KAAGGAAADLHALFVAHARELMEPVAPPAAA"
     gene            complement(4228..5175)
                     /locus_tag="VFB52_05965"
     CDS             complement(4228..5175)
                     /locus_tag="VFB52_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO05913.1"
                     /translation="MRIEQLEYLISVARHGSFRRAAEELHISQPALSETVRRLEQELG
                     VDILDRRRSGATISESGRVLLPYMQSAVEAIDQLRRVADDQHRNSRVVRLGTVTAATV
                     PLMTPTIHQFRATHEETRVEILTAQQDQIHQALLEGGMDLGLVNYLAGDDMPPEFEAT
                     ELLRGRPVVCIPAGHPLATETRVRPEQLASQPLVAMRSGYVMHRYLHRLLGEEERSFS
                     YAADGAEMGKLMVAAGLGVAVLPDYSVIGDPLTERGDISYRAIEGDETEVLLILHRRR
                     SGSTPKPVRDLHELFVKRARAFAHAGLATGEHEAAKQAA"
     gene            5362..6411
                     /locus_tag="VFB52_05970"
     CDS             5362..6411
                     /locus_tag="VFB52_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO05914.1"
                     /translation="MIELTELTKVFERGETRHAALNGVSLTVERGEIAAVIGPSGAGK
                     STLARCINMLERPTSGRVVLDGEDLTALRERHLREARRSIGTVFQASSLLSRKTAAQN
                     VALPLDFLEVDRRQRDERVHELLDRVGLGDRGDAYPHELSGGQRQRVGIARALALRPS
                     VLLSDEATSGLDPATTKSVLAMLSQMRDDLSLTIILITHEMDVVREVADRAALLEEGR
                     IVEEGRLQQLMRDPASALGGSLLPHPTPAAADGEREVWRVHYVSDRVDPDWVGNLSRQ
                     LDAPIALLAASIEAVGADPVGRATIALPPRDPQLVRDQLAAAGLHGEPVAGGPAAAAE
                     QPPSATPAPALSEAA"
     gene            6411..>6731
                     /locus_tag="VFB52_05975"
     CDS             6411..>6731
                     /locus_tag="VFB52_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZO05915.1"
                     /translation="MLVLATNTPLGELPDLLLPAIGQTLQMVGVTMAIVVALGTPLGV
                     ALHNSSRGGLFPRPKLNAVLSWITNLGRSLPFLVLMASIIPFTRLLVGSTIGIWAAVV
                     PMTVA"
BASE COUNT          988 a         2410 c         2344 g          989 t
ORIGIN      
        1 tcgtctcgac cgacgcggag gcgatggcgg cgctcgcctc cagtctcgac ctgatcgtcg
       61 acacggtcgc cgcgccgcac gacctcgatc cctacctggc gaccctgggg cgcgagggcg
      121 ccctcgtcct ggtcggaatc cctcccgagt ccgaacccca tccctcgccc agcgcgctcg
      181 gtctgatcag ccagcgccgc accctgaccg gctcctcgat cggcgggatc gccgagaccc
      241 aggagatgct cgatttctgc gccgagcacg ggatcgtcag cgagatcgag ccgattgcga
      301 tcaaccagat caacgacgcc tacgagcgca tgctcgccgg cgacgtccgc ttccgcttcg
      361 tcatcgacat gtccaccctg agctaggagc ccccgatgtc ccgactccgc ttcaacgcgt
      421 tcgcgatgaa ctgcgtctcc cacatccacc acgggcagtg gacgcgcccg gacacgcgcc
      481 agaccgagta cgcgagcctc gacccctggg tcgagctggc gcagctcctc gagcggggga
      541 aatttgacgc gctgttcctc gccgacgtga tcggcaccta cgacacctac cggggcggcc
      601 gcgacacctc ggtcctcgag gggctgcaga tccccgtcaa cgacccctcg ctgatcatcc
      661 cggtgatggc gcacgagacc gagaacctcg gcttcgcctt cacccagtcg gtgctgcagg
      721 agcagccgtt caacttcgcc cgccgcctat ctactctcga ccacgtcact ggcggccgga
      781 tcgcctggaa cgtggtcacc tcctacctcg agaacgccgg gcgcaacctc ggctacggcg
      841 ggctgccgcc gcacgaggag cgctacgacc gcgccgacga gtacctcgag gtgatctaca
      901 agctgctgga gggaagctgg gatgccgacg ccgtcgtcgt ggaccgcgag cgcggggtct
      961 acgccgaccc ggcgaagatc cacaacatcg accacgccgg tcgcttctac gacgtggtcg
     1021 ggccgcacct ctccgagcct tcggcgcagc gcaccccggt catcttccag gccggctcct
     1081 ccgaccgcgg gcgcgagttc gcggcccaca acgccgaggc ggtcttcatc atcgcccgca
     1141 accccgaggg ggcgcgggcg cagaccgagg acatgagggc ccgtgtggtg cgccacgggc
     1201 gcgagcccgg cgacatccgc ttcttccagg cgctgagcgt gatcgtcggc gggaccgagg
     1261 aggaggcgtt gcgcaagcag gccgagatcg acgagcgggc cagcgacgag ggatacgccg
     1321 cccacatggc cggcaacatc ggcgtcgacc tcgccgacat cgacctcgag gcgccgctga
     1381 ccgagctgcc cgagcgctac gccggccaga gcatgatgaa cgtgctgatc gagtcggcgc
     1441 ccgacaagag ctgggccttc ggcgacctct tgcgctggat cgccagcgtg cgcgtcgtcg
     1501 gcacccctga gcagatcgcc gacgagctcg gtgtctacgc cgacgccggg gtcgacggga
     1561 tcaacctcat gtactacacg accccgggat cgttcgtcga cttcatcgac ggggtcgccc
     1621 cggtgctgca ggagcgcggc ctgatgcaga gcgagtaccg gccggggacg ctgcgcgaga
     1681 agctcagcga cggcgccgac ggcccgctga tctccgagcg ccacccggcc gccgggtacc
     1741 ggcgccgcca ggcggtcgcc gcatgagcag gcagcggctg atcgacaccg acgtccacga
     1801 gcgcgtcgag ctggagcagc tgctgccgca cctgccggcg atctggcaca agtacgtgac
     1861 cgactacggc tggacgccgg agcgcttcaa cccttacagc cagccgaccg ccggcgggct
     1921 cgcccgggct gacgcggtgc ccgagggcgg cggcctggcc gggtccagcc tgccgctgct
     1981 gcgcgagcag ctgctcgacg agtacgggat cgagcaggcg atcctcaccg gctggctcag
     2041 cgcctcggcc ctgcaggagg gctggagcga gttcaagacg gcgctgatgt ccgcctacaa
     2101 cgaccaccag gtggcgcact ggctggaccg cgaggagcgg ctctacggct cggtccaggt
     2161 caacgcctgg gaccccgagg gcgccgcccg cgagatcgag cggatggccg cgcacccgaa
     2221 gatcgtgcag gtgatgctct acgtcggcga ccaggcctac ggctcgccgc gctaccaccc
     2281 gatcttcgcc gcggccgagc gccacggcct cgtcgtcggc atccaccaca gcgaaaactc
     2341 gaccccggcg ctcggccgcc accgctacta cgccgagtgg cacacgctga tctcccaggt
     2401 cttcatgtcg caggcggtca gcctgatctt caacggcgtc ttcgaaaagt tcccggcgct
     2461 gaaggtgatc ctgatcgagg gcggcttctc ctacgtcccg cacctgatgt ggcgctccga
     2521 ccagcagtgg cgcgagctgc gccacgaggt gccctgggtg aagcgggcgc cgagccggat
     2581 catccgcgag caggtgcgct tcgccagcca gccgatcgag gagctgacca cgccgcagct
     2641 gctctcgctg atcgaccaga tgggctccga ggagctgatc tgcctctcca gcgactaccc
     2701 gcactgggac ttcgactcgc ccttcgaagc gctgccgccg ggcttccccg aggacctcaa
     2761 gcagaagatc ttctgggaga acgcgctcgc cacctacgcg cggctgccgg cggtggtcgg
     2821 cgaggccggc acccgggtcc cggcgtgagc caggcgaccg cgacgcgggc gcgcgccagc
     2881 ctgcccgccg ccgagctcgg gccgggggag atgaccggcc tggtggtcgg cgaggtagcg
     2941 gtggtcgtcg tgcgcacgac cgagggcgag ctgcgcgcct accgcgaccg ctgcgtgcac
     3001 cagggcgcgc cgctctcgcg cggccggctg cgggccggga ccgagggcga ggaggtcggc
     3061 gagtaccggc tggtcgaggg ccagggcgtg atcaagtgcc cctggcacgg ctacgagtac
     3121 gacgccgcca gcggctgtgc cctgttcgac tcgcgccgcc ggctgcgccg cgtgaccgtc
     3181 gaggaactcg acggcgagat cgtcgtctcg gtctgaggct tcaggccgcg gccggcgggg
     3241 ccactggctc catcagctcg cgggcgtggg cgacgaagag ggcgtgcagg tcggcggcgg
     3301 cgccgcccgc ggccttcgag cgccggcgct ggatcgtcag catcacgtcg gtgtcgtcgt
     3361 cgatctcgcg gaaggtgatc cggccgctct gttcgagcgg gtcaccgacc acgctgtagc
     3421 tggggagcac ggcgacgccg agcccttcgg cgaccatcat cttgcccatc tcggcgccgt
     3481 cggcggaata ggagaacttc ggtgtgtggc cgtcgagcag ccggttcacg tagcggtgca
     3541 tcaggtagcc ggtgcgcatc gcgaccagcg gctcgcccat caggtcctcg ggcccgatca
     3601 gcgccttcga ggcgagcggg ctgtcggcgc gcacgcagac gaccgggcgc ccgcgcagca
     3661 gctcggtggt ctccaggtcg ggcggcatgt cgtcgccgcc gaggtagttg acgaggccga
     3721 ggtcgagcgc gccgtcgagc aggtcgcggt ggatccagtg ctgctgggag gtgacgatct
     3781 cgatctgtgt cgaggggtgg gcctcggaga actcgcggat cgtcggggtc aggagcggcg
     3841 cggtcgccgc ggtcaccgtg ccgacccgga ccacgcggct gttgtggtgc tggtcgtcgg
     3901 cggcgccgcg cagccggtcg accgcctcga tcacctcgag gatgtggggg agcagctcgc
     3961 ggccgcggcc gctgatcgtg acccccgagc gcccgcggtc gaggatctcg acgccgagtt
     4021 caccctcgag cttgcgcacg gtctcgctga gtgccggctg ggagaggtgg agctcctcgg
     4081 cggcgcgacg aaatgagccc aaccgggcga cggcggtgac gtactcgagc tgctcaaatc
     4141 gcataggcgt caaacctcat acatagtatg ttgatcaact aagtcaatta ttgcgccgaa
     4201 agactacagg atgcgccggg gccggagcta ggccgcctgt ttcgcggcct cgtgttcgcc
     4261 ggtcgcgagc ccggcgtggg cgaaggcccg ggcccgtttc acgaacagct cgtgcaggtc
     4321 gcgcaccggc ttcggtgtcg agcccgagcg gcgccggtgc aggatcagga ggacctcggt
     4381 ctcgtcgccc tcgatcgcgc gataggagat gtcgccgcgc tcggtcagtg ggtcgccgat
     4441 cacgctgtag tcggggagga cggcgacgcc gagcccggcg gcaaccatca gcttccccat
     4501 ctcggccccg tcggcggcgt aggagaagga gcgctcctcc tcgccgagca gccggtgcag
     4561 gtagcggtgc atcacgtagc cggagcgcat cgccaccagc ggctggcttg ccagttgctc
     4621 ggggcggacc cgcgtctcgg tcgcgagcgg atggccggcg gggatgcaga cgacgggccg
     4681 gccgcgcagc agctcggtcg cttcgaactc gggcggcatg tcgtcgccgg ccaggtagtt
     4741 gaccaggccg aggtccatgc cgccctcgag cagggcctgg tggatctggt cctgctgggc
     4801 ggtgaggatc tcgacccggg tctcctcgtg ggtcgcgcgg aactggtgga tcgtcggcgt
     4861 catcagcggc acggtcgccg ccgtcaccgt gccgagccgg accacgcggc tgttgcggtg
     4921 ctggtcgtcg gcgacccggc gcagctggtc gatcgcctcg accgcgctct gcatgtaggg
     4981 gaggagaacg cggccgctct cgctgatcgt cgcccccgag cgcctgcggt cgaggatgtc
     5041 gacgccgagt tcctgctcca agcggcgcac ggtctcgctc agcgccggct gggagatgtg
     5101 cagctcttcg gcggcgcgcc ggaaggagcc gtgtcgggcc accgagatca ggtattcgag
     5161 ctgctcgatc cgcatggggg agcgaaagta tagctttttg atcgattaag tagggaaagg
     5221 ctatcggggg ccagggaatc cctatttgac ggcttgcctg agcgccgcca tcatccgccg
     5281 ccaccgttga ttgatgacta agtcagtcaa cttaatggtg aacctttcca gtccagatcc
     5341 cagtccgaaa ggggcggctc gatgatcgag ctgaccgagc tgaccaaagt gttcgaacgc
     5401 ggggagaccc gccacgcggc cctgaacggc gtcagcctca cggtcgagcg cggggagatc
     5461 gccgccgtga tcggtcccag cggggccggc aagagcaccc ttgcccgctg catcaacatg
     5521 ctcgagcgcc ccacctccgg gcgcgtcgtc ctcgacggcg aggacctgac cgcgctgcgc
     5581 gagcgccacc tgcgtgaggc ccgccgctcg atcggcaccg tcttccaggc ctccagtttg
     5641 cttagccgca agaccgcggc ccagaacgtc gcgctgccac tcgacttcct cgaggtcgac
     5701 cgccgccagc gcgacgagcg cgtgcacgag ctgctcgacc gggtcgggct cggcgatcgc
     5761 ggcgacgcct acccgcacga gctctccggc ggccagcgcc agcgggtcgg gatcgcccgc
     5821 gcgctcgccc tgcgcccctc ggtcctgctc tccgacgagg cgacctccgg cctcgacccg
     5881 gcgacgacca agtcggtgct ggcgatgctg agccagatgc gcgacgacct cagcctgacg
     5941 atcatcctga tcacccacga gatggacgtg gtccgcgagg tcgccgaccg cgcggcgctg
     6001 ctcgaggaag ggcggatcgt cgaggagggc cggctgcagc agctgatgcg cgacccggcc
     6061 tcggcgctcg gcggctcgct gcttccccac ccgaccccgg ccgcggccga cggcgagcgc
     6121 gaggtctggc gggtccacta cgtctccgac cgggtcgacc ccgactgggt cggcaatctc
     6181 tcccgccaac tcgacgcgcc gatcgcgctg ctcgccgcct cgatcgaggc ggtcggcgcc
     6241 gatccggtcg ggcgggcgac gatcgcgctg ccgccgcgcg acccgcagct cgtccgcgac
     6301 cagctcgccg ccgccggcct gcacggcgag ccggtcgccg gcgggcccgc cgcggccgct
     6361 gagcagcccc cgtccgccac cccggctccc gcgctcagcg aggccgcctg atgctggtcc
     6421 tcgccaccaa caccccgctc ggcgagctgc cggacctgct gctgccggcg atcgggcaga
     6481 cgctgcagat ggtcggcgtg acgatggcga tcgtcgtcgc gctcggcact ccgctcggcg
     6541 tcgcccttca caactcctcc cgcggcggcc tcttcccgcg cccgaagctg aacgcggtgc
     6601 tgagctggat caccaacctc ggccgctcgc tgcccttcct ggtcctgatg gcctcgatca
     6661 tcccgttcac gcggctgctg gtcggctcga cgatcgggat ctgggccgcg gtcgtgccga
     6721 tgaccgtcgc c
//
LOCUS       DASRIG010000214         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1924920, whole genome shotgun sequence.
ACCESSION   DASRIG010000214 DASRIG010000000
VERSION     DASRIG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924920"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFB52_05980"
     CDS             complement(<1..581)
                     /locus_tag="VFB52_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO05916.1"
                     /translation="MSRPDEVAILSADRALSWRELEEASDRLAGGYRGLGLEPGDRIA
                     SLMPNRVDLVVHYLAAFKAGLVVTPLNYRYTHREIDHALTVSGAKLLVAHEERAEDVA
                     GSELASGLELGTIVFADGEEPELGKGSANPWHHSFAALLASDPIASESAVDPGEPAAI
                     FFTSGSTGPAKGVTHTRETLRWMIASAAAAFEL"
     gene            684..1370
                     /locus_tag="VFB52_05985"
     CDS             684..1370
                     /locus_tag="VFB52_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZO05917.1"
                     /translation="MDNGPIEAVVFDWDGTLVDSKQVLVEAFQASTEEVLGSPYPSTH
                     EETEMIVQLRGEDAFALLAEGDEALAARLAEAFHPHYVSRTENAVPFPGTLDTLQRLR
                     AAGLKLGVATSKARIRLDLEAERTGIGAYLDEEITGDEVKAAKPDPECVVEVIRRLGA
                     DPARTLFVGDGPNDVLAGQGAGAITVAVTFGFHPDEARAAGPDHVIEAMPELLGLAGA
                     GPRDSLEAQT"
     gene            1455..3200
                     /locus_tag="VFB52_05990"
     CDS             1455..3200
                     /locus_tag="VFB52_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZO05918.1"
                     /translation="MKPMRSLARSRTEGMGFGATLAARAESEPELNLFTCLSDDLTEA
                     EEVSAAEAHRRAQTIAAGLRERQEVGSRTLLLFPPGLDYVCTVYGCFQAGVVGVSAPP
                     PQPNRLQRTLPRLTAIAADAQIDSVLTTAFIRDAAKPLFADGPLAATEWIAIDELPEP
                     DTELLGERPLTELAFLQYTSGSTRSPRGVMLSQENLMVNSDLIAEAFGHSPGEARGVI
                     WLPPYHDMGLIGGILQPVHAGGNCVLLSPLAVVKRPARWLQAISRYGATTSGGPNFAY
                     DLCVQRVDEEIVETLDLSSWRVAFNGAEPIRAQTIEQFARKFSSAGFRREAFYPCYGL
                     AEATLIVTGPDREDEPSLLEVDSAAVERGRIEPSSGESGTRTLVGCGTVFADHELAIV
                     DPESGEPCREGEIGEVWTAGPSVALGYWNQEDETAEQFGATLDGESDGRWLRSGDLGA
                     LVDGELFVVGRIKELIIANGRNIHPHDVEWSAEEAHPALRRHCSAAYDRADDGPAEVA
                     LLLEVDGAEELDPAAVIAAVRERVAADLDLPLAWVGLCGRGEIPKTTSGKIQRNLCRR
                     MVAAGEFGLIAEAER"
     gene            3197..3847
                     /locus_tag="VFB52_05995"
     CDS             3197..3847
                     /locus_tag="VFB52_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO05919.1"
                     /translation="MSGVCAPVVAAGMRDYYERRAEEYDEWWLGNGGFLERERPGWPE
                     EVVGLITLLSSLPSARTLDVACGTGFLTQHLAGEVTGLDQSEAMIARASERCPEREFV
                     CSDAVPLPFEDDTFELVFASHFYGHLLAEERRRFLAEAARVGRRLVVVDAALRADVRP
                     RQWQRRELNDGSVHRIYKRYFTPAELAAEVGGATVLHAGDWFVAVERELGSGDALA"
     gene            3834..4247
                     /locus_tag="VFB52_06000"
     CDS             3834..4247
                     /locus_tag="VFB52_06000"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZO05920.1"
                     /translation="MPSPSQPLLGSCLCGGVRLQITGPFLRASYCHCHRCQKRTGGAA
                     SAQGRVAQRDFELLAGHDLMKSYTVSEGTRPKVFCLRCGSALFSGDPLTDDEVSVRLG
                     LLDGDPGITPQVHSFASSAPAWAPVPDDGLPRVPE"
     gene            4244..5053
                     /locus_tag="VFB52_06005"
     CDS             4244..5053
                     /locus_tag="VFB52_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05921.1"
                     /translation="MKLLVVKVGGSLFSDKRRARTLDETALDAYAAAIAELARLAPGR
                     VVFVSGGGAFGHDAVRVLDPGDPDAALPLTEAVFALKWRWTEALRAHGCRALPLQATS
                     LAYRHDGRLQIHAGAVAGLLADGILPVLSGDAIGGDGRLEVVGSDRVAGLLLGLAPGA
                     VRVAMLTDVPGVLTDGRGGSRVLREVDPDHPERALAAVWETPAWDTSRSMDGKLAAAI
                     ELARGGAECLIMAGEPRPQLLRLLLEPCERWPRSVRYTRISRAAAGAAAAG"
     gene            complement(5016..5498)
                     /locus_tag="VFB52_06010"
     CDS             complement(5016..5498)
                     /locus_tag="VFB52_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZO05922.1"
                     /translation="MTPEQAARGYIESFSEHDVGRALAHWARGGRQHVRGQIDTVAPD
                     GVREQIEALIAAAPDFRFEVVGTVSEGERCALHWRLRGHFNGSARLYGVRPTGDRLEL
                     EGVDIFTVRDGLIVANDAFSDSMEFTRQIGMMPPRGSRRERFFTALFNLRPRRRRRRG
                     "
BASE COUNT          788 a         2123 c         1997 g          761 t
ORIGIN      
        1 ctcagctcga aggccgcggc ggcgctggcg atcatccagc gcagggtctc gcgcgtgtgg
       61 gtgacgccct tggccgggcc ggtgctgccc gaggtgaaga agatcgctgc gggctcgccg
      121 gggtcgacgg cggactcgga cgcgatcggg tcgctggcca gcagagcggc gaagctgtgg
      181 tgccagggat tcgccgaacc cttcccgagc tccggctcct cgccgtcggc gaagacgatc
      241 gtgcccagct ccagaccgct cgccagctcg ctgccggcga cgtcctcggc ccgctcctcg
      301 tgcgcgacca gcagcttggc gccgctgacc gtgagcgcgt ggtcgatctc gcggtgggtg
      361 tagcggtagt tgagcggcgt caccaccagg ccggccttga acgcggcgag gtaatggacc
      421 acgaggtcga cccggttcgg catcagcgag gcgatccgat cccccggttc cagcccgaga
      481 ccccggtagc cgcccgccag ccgatcgctc gcctcctcca gctcgcgcca gctcagcgcc
      541 cggtcggcgg agaggatcgc gacctcgtcg ggccggctca aaccggcggc aagaagctcg
      601 tttagaggtc cacacggaga ggcgagcgga ttcccgtcga tcagcatcgc cggaaacgta
      661 cactcgccgg catcggagcg aaagtggaca acgggccaat cgaagcggtg gtcttcgact
      721 gggacgggac cctggtcgac tccaagcagg tcctggtcga ggccttccag gcgagcaccg
      781 aggaggtgct cggcagcccc tacccgagca cgcacgagga gaccgagatg atcgtgcaac
      841 tgcgcggcga agacgccttc gcgctgctcg ccgagggtga cgaggcgctc gccgcccgcc
      901 tcgccgaggc cttccacccc cactacgtct cgcgcaccga gaacgcggtc ccgttcccgg
      961 gcaccctcga caccctgcag cgcctgcgcg ccgccggcct caagctcggc gtcgccacct
     1021 cgaaggcgcg gatccgcctc gacctcgaag ccgagcgcac cggaatcggc gcctacctcg
     1081 acgaggagat caccggcgac gaggtgaagg cggccaagcc cgaccccgag tgcgtcgtcg
     1141 aggtgatccg ccgcctcggc gccgaccccg cccgcaccct cttcgtcggc gacggcccca
     1201 acgacgtcct cgccggccaa ggagccggcg ccatcaccgt cgccgtcacc ttcggcttcc
     1261 accccgacga ggctcgcgcc gccggccccg accacgtgat cgaggcgatg ccggagctgc
     1321 tcggtctggc cggcgccggg ccccgagact cgctcgaagc tcaaacctga gctgaccaaa
     1381 ctggggagaa agggggtcgg ggggtgcgca gcttggtgtc gcgagcctgc ctttcgcact
     1441 ggtacttctg aacgatgaaa ccaatgcgat cgctggcgcg ttcgcgcacc gagggcatgg
     1501 gcttcggcgc caccctcgcc gcgcgcgccg agagcgaacc cgagctgaac ctgttcacct
     1561 gcctctccga cgacctcacc gaagccgagg aggtgagcgc ggcggaagcc caccggcgtg
     1621 cccagacgat cgccgccggc ctgcgcgagc gccaggaggt gggcagccgc acgctgctcc
     1681 tcttcccccc cggcctcgac tacgtgtgca cggtgtacgg ctgcttccag gcaggcgtcg
     1741 tcggcgtctc ggcgccccca ccgcagccca accggctgca gcgcacgctg ccgcggctga
     1801 ccgcgatcgc cgccgacgcg cagatcgact cggtcctcac caccgccttc atccgcgacg
     1861 ccgcgaagcc cctgttcgcg gacggccccc tcgcggccac cgagtggatc gcgatcgacg
     1921 agctgccgga gcccgacact gagctgctcg gcgagcggcc cctcacggag ctcgcttttc
     1981 tccagtacac ctcgggctcg acccgcagcc cccgcggggt gatgctgagc caggaaaacc
     2041 tgatggtcaa ctcggacctg atcgcggagg ccttcggcca ctcgccgggt gaggcgcggg
     2101 gggtgatctg gctgccgccg taccacgaca tggggctgat cggagggatc ctgcagccgg
     2161 tccacgccgg cggcaactgc gtgctgctct cgccgctggc cgtggtcaag cggccggcgc
     2221 gctggctgca ggcgatctcg cgctacggcg cgaccaccag cggcgggccc aacttcgcct
     2281 acgacctctg cgtgcagcgg gtcgacgagg agatcgtcga gacgctggac ctctccagct
     2341 ggcgggtcgc cttcaacggc gccgagccga tccgcgcgca gacgatcgag cagttcgccc
     2401 gcaagttctc ctcggccgga ttccggcgcg aggccttcta cccctgctac ggcctcgccg
     2461 aggcaacgct gatcgtgacc ggccccgacc gcgaggacga gccctcgctg ctcgaggtcg
     2521 actcggcggc ggtcgagcgc ggccggatcg agccttcgag cggcgagagc ggtacccgca
     2581 cgctggtcgg ctgcgggacc gtcttcgccg accacgagct ggcgatcgtc gaccccgaga
     2641 gcggcgagcc ctgccgcgag ggagagatcg gcgaggtctg gaccgccggc cccagcgtcg
     2701 cgctcggcta ctggaaccag gaggacgaaa ccgccgagca gttcggcgcc accctcgacg
     2761 gtgagagcga cggccgctgg ctgcgcagcg gcgatctcgg cgccctggtc gacggcgagc
     2821 tttttgtggt cggccggatc aaggagctga tcatcgccaa cggccgcaac atccacccgc
     2881 acgacgtcga gtggagcgcc gaggaggcgc acccggcgct gcggcgccac tgcagcgccg
     2941 cctacgaccg cgccgacgac ggcccggccg aggtcgcgct cctgctcgag gtcgacggcg
     3001 ccgaggagct cgaccccgcc gcggtgatcg cggcggtgcg cgagcgagtc gccgccgacc
     3061 tcgacctgcc actcgcctgg gtcgggctct gcggccgcgg tgagatcccg aagacgacca
     3121 gcggcaagat ccagcgcaac ctctgccgcc gcatggttgc cgccggcgag ttcgggctga
     3181 tcgcggaggc ggagcgatga gcggggtctg cgccccggtc gtcgccgcgg ggatgcgcga
     3241 ttactacgag cgccgcgccg aggagtacga cgagtggtgg ctcggcaacg gcggcttcct
     3301 cgagcgcgag cggccgggct ggcccgagga ggtggtcggg ttgatcaccc tgctcagctc
     3361 cctgccctcc gcgcgcacgc tcgacgtcgc ctgcggcacc ggcttcctca cccagcacct
     3421 cgccggcgag gtaaccgggc tcgaccagag cgaggcgatg atcgcccgcg cctccgagcg
     3481 ctgtcccgag cgcgagttcg tatgctccga cgccgtgccg ctgccctttg aggacgacac
     3541 cttcgagctc gtcttcgcca gccacttcta cggccacctg ctggccgagg agcggcgccg
     3601 cttcctcgcg gaagcggcgc gcgtcggccg tcgcctggtc gtcgtcgacg cggcgctgcg
     3661 tgccgacgtc cggccccggc agtggcagcg gcgtgagctc aacgacggct ccgtgcaccg
     3721 gatctacaag cgctacttca ccccggcgga gctggcggcc gaggtcggcg gcgccaccgt
     3781 cctgcacgcc ggcgactggt tcgtcgcggt cgaacgcgag ctcgggagcg gcgatgccct
     3841 cgcctagcca gccgctcctc ggctcctgcc tctgcggcgg cgtgcgcctg cagatcaccg
     3901 ggcccttcct gcgcgccagc tactgccact gccaccgctg ccagaagcgc acgggcggcg
     3961 ccgcctcggc ccaggggcgg gtcgcgcaac gggacttcga gctgctcgcc ggccacgacc
     4021 tgatgaagtc ctacacggtc agcgagggga cccggccgaa ggtcttctgc ctgcgctgcg
     4081 gctcggccct gttcagcggc gacccgctga ccgacgacga ggtgagcgtg cgtctcggtc
     4141 tgctcgacgg cgacccgggg atcaccccgc aggttcactc cttcgccagc tcggcgccag
     4201 cctgggcgcc ggtccccgac gacgggctgc cgcgcgtccc ggaatgaagc tgctcgtcgt
     4261 caaggtcggc ggcagcctgt tctccgacaa gcgccgggcc cgcaccctcg acgagaccgc
     4321 gctcgacgcc tacgcggcgg cgatcgccga gctggcgcga ttggccccgg gcagggtcgt
     4381 cttcgtcagc ggcggcggcg ccttcggcca cgacgccgtg cgcgtcctcg accccggcga
     4441 ccccgacgcg gcgctgccgc tgaccgaggc cgtcttcgcg ctcaagtggc gctggaccga
     4501 ggccttgcgc gcacacggat gccgggcgtt gccgctgcag gcaaccagcc tcgcctaccg
     4561 ccacgacggc cggctccaga tccacgccgg agcggtcgcg gggttgctcg ccgacggcat
     4621 cctgccggtg ctctccggcg acgcgatcgg cggcgacggg cgcttggagg tggtcggcag
     4681 cgaccgcgtc gccggcctgc tcctgggact cgcgccgggc gccgtgcggg tggcgatgct
     4741 gaccgacgtg ccaggagtgc tcaccgacgg ccgcggcggc tcgcgggttc tgcgcgaggt
     4801 tgaccccgac caccccgagc gtgcgctcgc cgccgtctgg gagacgcccg cctgggacac
     4861 cagccgctcg atggacggca agctggccgc cgcgatcgag ctcgcccgag gcggcgccga
     4921 gtgcctgatc atggccggcg agccacggcc ccagctgctg cgcctgctgc tcgagccgtg
     4981 cgagcgctgg ccgcgctcgg tccgctacac gcggatcagc cgcgccgccg ccggcgccgc
     5041 ggccgcaggt tgaagagcgc ggtgaagaaa cgctcgcggc gcgatccgcg cggcggcatc
     5101 atcccgatct ggcgggtgaa ctccatcgag tccgagaagg cgtcgttggc gacgatcagg
     5161 ccgtcgcgga cggtgaatat gtcgaccccc tccagctcca gccgatcacc ggtcggccgt
     5221 acgccgtaga gccgcgccga gccgttgaaa tggccgcgca ggcgccagtg cagggcacag
     5281 cgctcgccct cgctgacagt cccgacgacc tcgaagcgaa agtcgggcgc ggcggcgatc
     5341 agggcctcga tctgctcgcg caccccgtcg ggggcgaccg tgtcgatctg gccgcgcacg
     5401 tgctgtcgcc caccccgcgc ccagtgggcg agcgctcgcc cgacgtcgtg ttcggagaag
     5461 gactcgatgt agccgcgagc ggcctgctcg ggggtcacgc tgggctccgc tcgcgcgcgc
     5521 ggcgccagcg cgccggcggt gcgtcaccga gcgtctgccg ctcgacgccg ggctcgtcga
     5581 actcgatctc gccgacagca gcggcgacct cttcccagag ccccggagcg ccggcgcggg
     5641 cggcatcgag cagcggccgc tgagcggcg
//
LOCUS       DASRIG010000215         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2181821, whole genome shotgun sequence.
ACCESSION   DASRIG010000215 DASRIG010000000
VERSION     DASRIG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181821"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1042)
                     /gene="corA"
                     /locus_tag="VFB52_06015"
     CDS             complement(35..1042)
                     /gene="corA"
                     /locus_tag="VFB52_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZO05923.1"
                     /translation="MPNLPKPRLRRPGRRGELVVPAPAAEPQAPQVEQIEAAGLRWVN
                     IERPGGLERTWLEEHFEFHALDLEDVLSRNQRPKIDVYDDYLFIVLHFPIFDRAAGRL
                     GTGELDLFVGPDYMVTIPNQPLQPVEYLFERCRSDEDLREKLFSRGSGYLLYRLVDDS
                     FDYCFPMLRKIGNKLDALEDDIFSGRSEEVVRDISNVKQEIINFRKVIRPQRPVLRDL
                     ERVKQRYLATDLDLEIYFDDIVDAHERIWDMLENYKEVAEALEETNESVISHRVNGIL
                     KVLTSISVIVLPLTLIASIWGMNVGVPGEGDSDDFYAIVGAMVVLMVGMIAFFRRRGW
                     L"
     regulatory      1152..1275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1345..2577
                     /gene="thrC"
                     /locus_tag="VFB52_06020"
     CDS             1345..2577
                     /gene="thrC"
                     /locus_tag="VFB52_06020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZO05924.1"
                     /translation="MSIENLKCKECGSTYELGASYFCEQCFGPLEVSYDFSELDPAEV
                     KRKIQTGSQGIWRYSDFLPFAGRPGDPLEPGLTPLLRADRLAEQIGTDAEIWIKNDAA
                     NPTHSFKDRVVAVAVAKARELGFDTVACASTGNLANAVAAHAAAAGLDSYVFVPANLE
                     EQKLLATGVYGTNLVGVRGNYDDVNRLCTQLAETRPWAFVNVNLRPYYAEGSKTLAYE
                     VVEQLGWELPDRVVCPIASGSLFTKLGRGFQEWLDLGLVEGEQPTFNGAQASGCSPVA
                     TAFSEGWDVCKPQKPDTIAKSLAIGDPADGPYAVEHARRTGGGIDSVSDQEIREAIKL
                     LAETTGIFTETAGGVTVGVLKRLAERGELAAGERVVVYLTGEGLKTLDATRDTFQMHE
                     IDPALESFEAEFRQEVTV"
     gene            2589..2849
                     /locus_tag="VFB52_06025"
     CDS             2589..2849
                     /locus_tag="VFB52_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZO05925.1"
                     /translation="MKIPTQLRAATGGEAELEVSGGTVGEVLDAVFDAHSELRERITQ
                     DGDLRRFVNVYVSGEDIRFQQGLDTAINDGDEVTILPAVAGG"
     gene            2880..3611
                     /locus_tag="VFB52_06030"
     CDS             2880..3611
                     /locus_tag="VFB52_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZO05926.1"
                     /translation="MRPPVVILCGGRGTRLRERTESVPKALVEIGGRPILWHVVQIYA
                     AQGFERFLLATGYLGEAVEEFAAAQRWPDGVEVECIDTGLDTPTGGRIARLGERLDGA
                     PFCATYADGVADVDLGAQLDFHRERGALATMTVVRPDLQWGVAELDGDSVAGFVEKPR
                     SERWINGGFFCFEPGVLGYLDEESVLEREPLARLAAEGELRAFRHEGFWDCMDTYKDA
                     VVLNDLWASGEAPWRSWDAAAVEQG"
BASE COUNT          592 a         1175 c         1282 g          579 t
ORIGIN      
        1 gatccggtgg ttggccacgt ccccgatgct agggctagag ccagccccta cgccggaaga
       61 aggcgatcat cccgaccatc aggacgacca tcgcgccgac gatcgcgtag aagtcgtcgg
      121 agtccccctc gcccgggacg cccacgttca tcccccagat gctcgcgatc agggtgagcg
      181 gcaggacgat gacgctgatc gaggtcagca ccttgaggat gccgttgacg cggtgggaga
      241 tcaccgactc gttggtctcc tcgagcgcct cggcgacctc cttgtaattc tccagcatgt
      301 cccagatccg ctcgtgggcg tcgacgatgt cgtcgaagta gatctcgagg tcgaggtcgg
      361 tcgccaggta gcgctgtttg acccgctcga ggtcgcgcag gaccggccgc tgcggacgga
      421 tcactttgcg gaagttgatg atctcctgct tgacgttgga gatgtcgcgc acgacctcct
      481 cggagcggcc gctgaagatg tcgtcctcga gcgcgtcgag cttgttgccg atcttgcgca
      541 gcatcgggaa gcagtagtcg aagctgtcgt cgaccaggcg gtagagcagg tagccggagc
      601 cgcgcgagaa gagtttctcc cgcaggtcct cgtcgctgcg gcagcgctcg aacaggtact
      661 cgaccggctg cagcggctgg ttcgggatcg tgaccatgta gtcgggtccg acgaagaggt
      721 cgagctcgcc ggtgccgagg cggccggcgg cacggtcgaa gatcgggaag tggaggacga
      781 tgaacaggta gtcgtcgtag acgtcgatct tcgggcgctg gttgcgcgag agcacgtcct
      841 cgaggtcgag ggcgtggaac tcgaagtgct cctccagcca ggttcgctcc aggcccccgg
      901 ggcgctcgat gttgacccag cgcagcccgg cggcttcgat ttgctcgacc tgaggagcct
      961 ggggctcagc tgcaggggcg gggacgacca gctcgccgcg ccggccaggc cgccgcagcc
     1021 gaggtttcgg aaggttcggc atcgggctgg ctcgcggtct cgtgcagcaa ggtcatcgtt
     1081 cacctaggtt agccgcaagc cgtgcctgca aacgtccgtg gggcggctgg taagcttctt
     1141 gccacataag cgccacatct agaggggtgg agggactggc ccagtgaagc cccggcaacc
     1201 acctcggatt tcgatccgag gggaggtgcc aactccagca ggtcgagcag tttcagttgg
     1261 cctggaagat gtgggggacg tccttttttc gggacctctg ccccacaagc ggcggaggtc
     1321 ttttttgatg gaaaagagga acggatgtca atcgagaact tgaagtgcaa ggagtgcggc
     1381 tcgacctacg agctcggcgc cagctacttc tgcgaacagt gtttcggccc gctggaagtc
     1441 agctacgact tctccgagct cgacccggcc gaggtcaaaa ggaagatcca gaccggctcg
     1501 caggggatct ggcgatacag cgactttctc ccgttcgccg gtcgtcccgg cgacccgctg
     1561 gagccgggtc tgacgccgct gctgcgcgcc gaccgcctgg ccgagcagat cgggaccgac
     1621 gcggagatct ggatcaagaa cgacgccgcc aacccgaccc actcgttcaa ggaccgggtc
     1681 gtcgccgtcg ccgtcgcgaa agcgcgtgag ctcggcttcg acaccgtcgc ctgcgcctcg
     1741 accggcaacc tcgccaacgc ggtcgctgcc cacgcggccg ccgccggcct cgactcctac
     1801 gtcttcgtcc cggcgaacct ggaggagcag aagctgctcg cgaccggcgt ctacgggacc
     1861 aacctggtcg gcgtgcgcgg caactacgac gacgtcaacc ggctctgcac ccagctcgcc
     1921 gagacgcggc cgtgggcctt cgtcaacgtc aacctgcgcc cctactacgc ggagggctcg
     1981 aagacgctcg cctacgaggt cgtcgagcaa cttggctggg agctgcccga ccgggtcgtc
     2041 tgcccgatcg cctcgggttc gctgttcacg aaactgggcc gcggtttcca ggagtggctc
     2101 gacctcggcc tggtcgaggg cgagcagccg accttcaacg gcgcccaggc gagcggctgc
     2161 agcccggtgg cgaccgcctt ctcggaaggt tgggacgtct gcaagccgca gaagccggac
     2221 acgattgcca aaagcctcgc gatcggcgat cccgccgacg gcccctacgc ggtcgagcac
     2281 gcgcgccgca ccggcggcgg catcgactcg gtctccgacc aggagattcg cgaggcgatc
     2341 aagctgctgg ccgaaacgac cggcatcttc accgagaccg ccggcggcgt caccgtcggg
     2401 gtcctgaaga ggctggccga acgcggcgag ctggcggccg gcgagcgggt cgtcgtctac
     2461 ctgaccggcg agggcctgaa gacgctcgac gccacccgcg atacgttcca gatgcacgag
     2521 atcgatcccg cgctcgagag cttcgaggcg gagttccgcc aggaggtaac cgtctagtgg
     2581 caatcacggt gaagattccg acccagctgc gcgccgcgac cggcggcgaa gccgagctcg
     2641 aggtgagcgg ggggaccgtc ggcgaggtgc tcgacgccgt cttcgacgct cactccgaac
     2701 tgcgcgagcg gatcacccag gacggcgacc tgcgccgctt cgtcaacgtc tatgtctccg
     2761 gcgaggacat ccgcttccag cagggcctcg acaccgcgat caacgacggc gacgaagtaa
     2821 cgatcctgcc ggccgtggct ggcggctagg cgcacgcctc ctcagataca actcccgctg
     2881 tgagaccacc ggtggtgatc ttgtgcgggg ggcgggggac gcggctgcgt gagcggaccg
     2941 agtccgtgcc gaaggcgctg gtcgagatcg gcggccggcc gatcctctgg cacgtcgtgc
     3001 agatctatgc agcgcagggg ttcgagcgct tcctgctcgc gaccggctac ctcggcgagg
     3061 cggtcgagga gttcgccgcg gcgcagcgct ggcccgacgg ggtggaggtc gagtgcatcg
     3121 acaccggcct cgacacgccg accggcgggc ggatcgcgcg gctcggcgag cggctcgacg
     3181 gcgcgccgtt ctgcgcgacc tacgccgacg gggtcgccga tgtcgacctg ggggcgcagc
     3241 tcgatttcca ccgcgagcgc ggcgcgctgg cgacgatgac ggtggtgcgg ccggacctgc
     3301 agtggggagt ggccgagctc gacggcgact cggttgccgg cttcgtcgag aagccgcgca
     3361 gcgagcgctg gatcaacggc ggcttcttct gcttcgagcc gggggtgctc ggctatctcg
     3421 acgaggagag cgttctggag cgcgagccgc tggcgcggtt ggccgccgag ggcgagcttc
     3481 gggccttccg ccacgagggc ttctgggact gcatggacac ctacaaggac gcggtcgtgc
     3541 tcaacgatct ctgggcctcc ggcgaggcgc cctggcggag ctgggacgcg gccgcggtgg
     3601 agcagggttg aagcgggcgc tgctgacc
//
LOCUS       DASRIG010000216         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1411767, whole genome shotgun sequence.
ACCESSION   DASRIG010000216 DASRIG010000000
VERSION     DASRIG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411767"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..305)
                     /gene="tatA"
                     /locus_tag="VFB52_06035"
     CDS             complement(90..305)
                     /gene="tatA"
                     /locus_tag="VFB52_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZO05927.1"
                     /translation="MPNIGPLEIGIVLVIVLLIFGPKKLPELGKSLGRGIREFKGGIG
                     GGDDDEKDNKQVEDSKPVEEKTTEKSA"
     gene            complement(350..1117)
                     /gene="tatC"
                     /locus_tag="VFB52_06040"
     CDS             complement(350..1117)
                     /gene="tatC"
                     /locus_tag="VFB52_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZO05928.1"
                     /translation="MRRVKAIAPEDQLSLVEHLDELRARIVVSVAVFGVALALCFWQN
                     SLLLELASGPLPSDHDQLITFGVTEPFTTTMTVAAYGAIILSLPIILFQMYAYLLPAF
                     SREQQRAILPLLLLIPALFIAGVAFGYFVVLPAAASFLLNFNDAQFNIQVRAKDYYGF
                     FAQTVLATGIVFQIPVGILAVTRLGIVSVDQLKKNRRYAYLGCAVIAAALPGIDPVSM
                     LLETAPLLALYELSIVLARVFGRPAAAPLPEAPGGRA"
     gene            complement(1128..1328)
                     /locus_tag="VFB52_06045"
     CDS             complement(1128..1328)
                     /locus_tag="VFB52_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZO05929.1"
                     /translation="MPNIGPMEIALVLIIALIVFGPKKLPELGKSLGRGIQEFKGTIS
                     GGDDEPAAKPQVEAETAAKPQH"
     gene            complement(1397..2119)
                     /locus_tag="VFB52_06050"
     CDS             complement(1397..2119)
                     /locus_tag="VFB52_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO05930.1"
                     /translation="MSTDNELAQIDSGGAVAEAAHEAVEGLGADDSRRGFLKKAGLAG
                     GALVGGGALLTALTPAGAMAAGKGRPPASFGKGDIGILNYALTLEYLEAAFYNEATAN
                     NNKKSFLKTAQEKAFLKQTTNDENAHVKFLKKALGKKAVAAPKVDFGATTGSRDAFIK
                     TAVALENTGVGAYSGQALNITSPAYIAAALSIWSVEARHASVAGLLLKPTPANISPDG
                     AFDQPKTADEVLKAVGKTGFLV"
     gene            2282..>2545
                     /locus_tag="VFB52_06055"
     CDS             2282..>2545
                     /locus_tag="VFB52_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZO05931.1"
                     /translation="MNSNLTTRKAPEQDAALRVCRAKAELRDALAPPRRAGRTIGLVP
                     TMGYLHDGHVSLLRAAHAECDLVVMSLFVNPTQFGPGEDLDRYP"
BASE COUNT          378 a          803 c          899 g          465 t
ORIGIN      
        1 tccccccgtt caccgtcgcc gccggcgctc cggccaaaca gatccgcaag atcgagctca
       61 gcgcctccga cggagacgct tagccgccgt taggcgctct tctcggtcgt cttctcctcg
      121 accggcttcg agtcctcgac ctgcttgttg tccttctcgt cgtcgtcgcc tccgcctatt
      181 ccgcctttga actcgcggat cccgcggccc agcgacttgc cgagttcggg cagctttttc
      241 ggcccgaaga tgagcaggac gatgaccaag acgatgccga tctcgagtgg tccgatgttt
      301 ggcataggga catgttcgca gggtcgacgg acccgcgcgc ggaagcccgc taggcgcggc
      361 cccccggtgc ctcgggcagc ggcgcagccg ccggtcgccc gaagaccctg gcgaggacga
      421 tgctgagctc gtagagcgcc agtagcgggg ccgtctccag cagcatcgag accgggtcga
      481 tccccggcaa cgcggcggcg atcacggcgc agccgaggta ggcgtagcgg cggttcttct
      541 tcagctggtc gacgctgacg atgccgagcc gggtgaccgc gaggatcccg acggggatct
      601 ggaagacgat cccggttgcg agcaccgtct gggcgaagaa gccgtagtag tccttggccc
      661 gcacctggat gttgaactgg gcgtcgttga agttgagcag gaagctggcc gcggcgggca
      721 ggacgacgaa gtagccgaac gcgaccccgg cgatgaacag ggccgggatc aacagcagca
      781 gaggcaggat cgcgcgctgc tgttcgcggc tgaaggccgg cagcaggtag gcgtacatct
      841 ggaacaggat gatcggcagc gagaggatga tcgccccgta ggcggcgacg gtcatcgtcg
      901 tcgtgaacgg ctcggtcacc ccgaaggtga tcaactggtc gtggtcggag gggaggggcc
      961 cgctcgccag ctccaggagg agcgagttct gccagaagca gagggcgagc gcgactccga
     1021 agacggcgac cgagacgacg atgcgcgcgc gcagctcgtc gaggtgctcg acgaggctga
     1081 gctggtcctc aggggcgatt gcctttacgc ggcgcatcgt gctgccgtca gtgctgcggt
     1141 ttggcagcgg tctcggcctc gacctgcggc ttcgcggcgg gctcgtcgtc gccaccgctg
     1201 atcgtgcctt tgaactcctg gattccccgg ccgagcgact tgccgagttc gggcagcttt
     1261 ttcggcccga agacgatcag ggcgatgatc aggactaggg ctatctccat cggtcctatg
     1321 ttgggcatgg cgatttctcc gatcgttgag cttgggggcg gccggattgc cgcccccggg
     1381 tgcggcgaat gtcgggctag accaggaagc cggttttgcc gactgccttg aggacttcgt
     1441 ccgccgtctt cggctggtcg aaggccccgt cgggcgagat gttggccggg gtcggcttca
     1501 gcagcagacc ggcgaccgag gcgtggcgcg cctccaccga ccagatcgaa agcgccgccg
     1561 cgatgtaggc ggggctggtg atgttcagcg cctggccgga gtaggcgccg acgccggtgt
     1621 tctcgagcgc gaccgccgtc ttgatgaagg cgtcgcgact gccggtggtc gcgccgaagt
     1681 cgactttcgg ggcggcgacc gcctttttgc ccagcgcctt cttgaggaac ttgacgtggg
     1741 cgttctcgtc gttcgtcgtc tgcttcagga aggctttctc ctgcgccgtc ttgaggaacg
     1801 attttttgtt gttgttggcg gtcgcctcgt tgtagaaagc ggcctccagg tactccagcg
     1861 tgagcgcgta gttgaggatg ccgatgtcgc ccttgccgaa gctggcgggc gggcgtcctt
     1921 tgcccgcggc catcgcgccg gcgggggtga gggcggtgag cagggcgccg ccgccgacca
     1981 gggcgccgcc ggccagtccg gccttcttga ggaagccgcg gcggctgtcg tcggcaccga
     2041 gcccctcgac cgcttcgtgt gctgcttcgg cgactgcgcc gccgctgtcg atctgtgcga
     2101 gctcgttgtc ggtactcatg gatcctcctg gcaatggggg gtggtggggg tgttgtcccg
     2161 attgcgagaa ggaggccggg gtagatcact ttcggccgat ttaaaagttc gctgcggggc
     2221 gtcgctgtcg cctgcctata attcgccact gatggccgtg accccgtagg gcatggacca
     2281 aatgaattcc aacctcacca ccaggaaagc ccccgagcag gacgctgccc tgcgcgtgtg
     2341 tcgcgccaag gccgagctgc gcgatgccct cgcgccgccc cgccgtgctg gacgcacgat
     2401 cggccttgtg ccgacgatgg gctacctgca cgacggccac gtctcgctgt tgcgcgcggc
     2461 ccacgccgag tgcgacctgg tcgtgatgag cctgttcgtc aacccgacgc agttcggacc
     2521 gggggaggac ctcgaccgct acccg
//
LOCUS       DASRIG010000217         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3773578, whole genome shotgun sequence.
ACCESSION   DASRIG010000217 DASRIG010000000
VERSION     DASRIG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773578"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFB52_06060"
     CDS             complement(<1..188)
                     /locus_tag="VFB52_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO05932.1"
                     /translation="MSEVELTWEGEEIAVLTLNRPESLNSMSRGLVSRLGELLTELKA
                     EGRARAVILTGAGRAFCAG"
     gene            complement(185..1234)
                     /locus_tag="VFB52_06065"
     CDS             complement(185..1234)
                     /locus_tag="VFB52_06065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZO05933.1"
                     /translation="MKVDFTGSFARLEDAGDSAAEAERRGYDGWWVTETKVDPPLACA
                     VAAQRSERIAIGTAVTIAFARNPMTVALQANDLQALSGGRFSLGLGSQIKAHVTKRFS
                     MPWGKPGSRMREFVRAIRAIWESWENGTPLAFEGEFYEHTLMTPFFDPGPNPYGNPDV
                     YLAAVGSRMTEIAGEVGDGLICHAFSTERYVREVTVPLLEDGRRAAGRAGESFELVAP
                     GFVVAADDPAELATGIASVKEQIGFYGSTPAYRPVLDLHGWGDLGDRLNRMSKRGEWE
                     QMAEAIDDEVVDAFAVVGSPEEVVAELARRYGDVATRLTLQLPERPDMDRWAPLLAQL
                     RGAGEQGKPTEEEQR"
     gene            complement(1231..>2399)
                     /locus_tag="VFB52_06070"
     CDS             complement(1231..>2399)
                     /locus_tag="VFB52_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO05934.1"
                     /translation="WVTMRRLLFEPEHEDFRASVRGFLSAEVVPEYGEWERRGIIPRE
                     AFLAAGAQGLLCPQVPEELGGPGVADLRFNAVLAEEIYLLGLSGYGSAITVQNDVCLP
                     YFLHFADAEQQRRWLPGLVSGETIAAIAMTEPGTGSDLAAIATSAVRDGEEYVVDGSK
                     TFITNGINADLVIVAARTERGEPRRGLTLLVVERGMEGFERGRNLEKIGMHSQDTAEL
                     YFDGVRVPAANRLGVEGDGFGQLTGNLAQERISIAVSAVAAARAALAAALDYTRERRA
                     FGKPIADFQSARFALAEAWAEIAVTQSFVDGCVLALAAGQLDAVDAAVAKLRATELQG
                     RVVDSCLQLHGGYGYMLEYPIARAYADARVSRIYGGANEIMKEIIGRSLVGGAR"
BASE COUNT          335 a          893 c          823 g          348 t
ORIGIN      
        1 cctgcgcaga aggcgcggcc ggccccggtg aggatcaccg cccgcgccct tccctccgcc
       61 ttcagctccg tcagcagctc acccagccgg gagactaggc cgcggctcat ggagttcagc
      121 gactcggggc ggttcagggt caggacggcg atctcctcgc cctcccaggt cagctcaacc
      181 tcgctcatcg ttgctcctcc tccgttggtt tcccttgttc tcccgcgccg cgcagctgcg
      241 ccagcagcgg tgcccagcgg tccatgtcgg gacgctcggg cagctgcagg gtgagccggg
      301 tggcgacgtc gccgtagcgg cgcgcgagct cggcgaccac ctcctccggg ctgccgacca
      361 cggcgaaggc gtcaaccacc tcatcgtcga tcgcctccgc catctgctcc cattcgcccc
      421 gcttcgacat ccggttgaga cggtcgccca ggtcccccca gccgtgcaga tcgagcaccg
      481 ggcggtaggc cggggtcgag ccgtagaagc cgatctgctc cttgaccgag gcgatcccgg
      541 tggccagctc ggccggatcg tcggcggcga cgacgaagcc cggcgcgacc agctcgaagc
      601 tctcgcccgc gcgcccggcg gcgcggcggc cgtcctccag gagcggcacc gtcacctcgc
      661 gcacgtagcg ctcggtcgag aaggcgtggc agatcagccc gtcgccgact tcgccggcga
      721 tttcggtcat ccgcgacccg accgcggcca ggtagacgtc cgggttgccg taggggttcg
      781 gccccgggtc gaagaacggg gtcatcagcg tgtgctcgta gaactcgccc tcgaaggcga
      841 gcggcgtgcc gttctcccag ctctcccaga tcgcccggat cgctcgcacg aactcgcgca
      901 tccgcgagcc cggtttgccc cacggcatcg agaaccgctt ggtcacgtgg gccttgatct
      961 gcgagccgag cccgagcgag aagcgacccc cggagagcgc ctggaggtcg ttggcctgca
     1021 gggcaaccgt catcgggttg cgggcgaagg cgatcgtcac cgcggtgccg atcgcgatcc
     1081 gctcgctgcg ctgcgccgcc accgcgcagg ccagcggcgg gtcgaccttg gtctcggtca
     1141 cccaccagcc gtcgtagccg cggcgctcgg cctcggcggc cgagtcgccg gcgtcctcga
     1201 gccgcgcgaa agagccggtg aagtcgacct tcaccgcgct cccccgacga gcgagcggcc
     1261 gatgatctcc ttcatgatct cgttcgcgcc gccgtagatc cggctcaccc gggcgtcggc
     1321 gtaggcgcgg gcgatcgggt actcgagcat gtagccgtaa ccgccgtgga gctgcaggca
     1381 gctgtcgacc acgcggccct gcagctcggt cgcccgcagc ttcgcgaccg ccgcgtcgac
     1441 cgcgtcgagc tgaccggcgg cgagcgccag cacgcaaccg tcgacgaagc tctgggtgac
     1501 cgcgatctcc gcccaggcct cggcgagcgc gaagcgagcg ctctggaagt cggcgatcgg
     1561 cttgccgaag gcgcggcgct cacgcgtgta gtcaagcgcc gcggccagcg ccgcgcgcgc
     1621 cgcggcgacc gccgagacgg cgatcgagat ccgctcctgc gccaggttcc cggtcagctg
     1681 gccgaaaccg tcgccctcca cgccgagccg gttggccgcc gggacccgca cgccgtcgaa
     1741 gtagagctcg gccgtgtcct gcgagtgcat cccgatcttc tccaggttgc gtccgcgctc
     1801 gaagccctcc atcccgcgtt cgacgacgag cagggtgagg ccgcgccgcg gctcgccgcg
     1861 ctcggtgcgc gcggcgacga tcaccaggtc ggcgttgatg ccgttggtga tgaaggtctt
     1921 ggagccgtcg acgacgtact cctcgccgtc gcggaccgcg ctggtcgcga tcgccgccag
     1981 gtcggagccg gtgccgggct cggtcatcgc gatcgccgcg atcgtctcgc cgctgaccag
     2041 gccggggagc cagcggcgct gctgctcggc gtcggcgaag tggaggaaat aaggcaggca
     2101 gacgtcgttc tggacggtga tcgccgagcc gtagccggag aggccgagca ggtagatctc
     2161 ttcggccagc accgcgttga agcgcaggtc cgcgacccca ggcccgccca gctcctcggg
     2221 cacctgcggg cagagcaggc cctgggcccc ggcggcgaga aaggcctcgc gcgggatgat
     2281 cccgcgccgc tcccactccc cgtactcggg gactacctcg gcactgagaa acccgcgcac
     2341 gctggcgcgg aagtcttcgt gctcgggctc gaagagaagc ctcctcatcg tcacccacc
//
LOCUS       DASRIG010000218         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_360837, whole genome shotgun sequence.
ACCESSION   DASRIG010000218 DASRIG010000000
VERSION     DASRIG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360837"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="VFB52_06075"
     CDS             <1..1864
                     /locus_tag="VFB52_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZO05935.1"
                     /translation="LRGAGGQVLRVEQLGAEARSVTVNLPGEPAPHNGEYSVEFWVNG
                     AVPTDKPLLGPGEIERLRFDNTRPGAAIPQLATTWVRGDTEPTVRIAHPVGPEPLARI
                     RGYAVSLHRDTAAPPCAGPDRCSVAETDLDGGVDDDTLALGLLPEGRYVVSAVAVSNS
                     GMRSALAETALVGIDATMPDLALEGAGGGWSDGPVRVIARASDTLSGMRATGPTGART
                     TIAVDGGVPTVAPGGEVATTVSGSGVHTVLAGARDAPGNVRGGQDGATPVAGIVRIDE
                     APPAVSFARRGDPADPELIEATVADALSGPGARGSIAVRPLGSGQPFEPLPTKAEGDR
                     LAARWDSDSYPEGAYEFRATGYDLAGNATSSTVRSSGAAMVLLNPVKVRTQLRFGFGG
                     RRLVWQRCVRKGESRRCRREVLESFARRPASRLVPYGRGLQVGGRLLTASGAPLAGAT
                     VELVEAFDPGAGIQSRVTSLRTDAEGGFLARLSPGPSRRIEARFGGDRLLTRATSRDL
                     RLGVQTLVRLRASTAGAEIGGAPVVFSGRVGASEATIPVYGRPVQLQFRLPGSPWTEF
                     RTVQTDAHGRFRFPYAFTDDDSRGVRFLFRAYAPAQPGWPYEPAASRPVAVTGR"
     gene            complement(1972..2400)
                     /locus_tag="VFB52_06080"
     CDS             complement(1972..2400)
                     /locus_tag="VFB52_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05936.1"
                     /translation="MKATKRAAVAEKNQVLTAAETAVDIPVGIVLSVSDRVSDLVEPW
                     TGRSSAEKQLKSYQSQLRKSLKRTERRGTSARRKATTEARKTRNRVEREARKRQRTVE
                     TTLKRNRAEVESRVRKAIDEQTSRASDLVGSVTDQLAALR"
     gene            complement(2672..>3234)
                     /locus_tag="VFB52_06085"
     CDS             complement(2672..>3234)
                     /locus_tag="VFB52_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZO05937.1"
                     /translation="DGSLNARRTIPSHSLSIAVASGSSMADVEFGFVHDFGADEEFVA
                     RRGEGASVRRGHVDGSVAESAAQVAAEGERLEVVGLESAEPQWSQPALAALDGKAYRL
                     RVVGSIAITAAYVGSGRFDAMFSLRPCRSVDAAAAQLFVREAGGEVAFGELALGEAPL
                     DLDARYPMAAASGAAGLATVRTAQSA"
BASE COUNT          435 a         1172 c         1152 g          475 t
ORIGIN      
        1 cctgcgcggt gccgggggac aggtgctgag ggtcgagcaa ctcggcgccg aggcgagatc
       61 ggtgaccgtc aacctcccgg gcgagccggc cccgcacaac ggcgaataca gcgtcgagtt
      121 ttgggtcaac ggagcggtgc cgaccgacaa accattgctg ggcccaggcg aaatcgagcg
      181 gttgcggttc gacaacacgc gccccggcgc ggcgatcccg cagctcgcga cgacctgggt
      241 ccggggcgac accgaaccga cggtgcggat cgcgcatccg gtcggccccg aaccgctcgc
      301 gaggatccgc ggctacgcgg tctcgctgca tcgcgacacc gccgcgccgc catgcgccgg
      361 gccggatcgg tgcagcgtgg cggagaccga cctcgacggc ggcgtcgacg acgacacgct
      421 cgccctcggg ctgctgcccg agggccgtta cgtggtcagc gccgttgccg tctccaactc
      481 cgggatgcgc tcggcgctcg ccgagacggc gctcgtggga atcgacgcga cgatgcccga
      541 cctcgctctg gagggagcag ggggcggctg gtcagatgga ccggtccggg tgatcgcgcg
      601 ggcgagcgac accctctcgg ggatgcgggc caccgggccg accggggcgc gcacgacgat
      661 cgctgtcgac ggcggcgtcc ccaccgtcgc gcccggcggg gaggtcgcga ccacggtcag
      721 cggcagcggc gtgcacaccg tgctcgccgg cgctcgcgac gcccccggca acgtgcgcgg
      781 cgggcaggac ggggccacgc cggtcgccgg gatcgtccgc atcgacgaag cgccgcccgc
      841 cgtctccttc gcccgccgcg gcgaccccgc cgacccggag ctgatcgaag ccaccgtcgc
      901 cgacgcgctc tccggaccgg gggcgcgagg ctcgatcgcg gtgcgcccgc tcggctccgg
      961 acaacccttc gagccgctgc cgaccaaggc cgagggcgac cggctggccg cccgctggga
     1021 ctcggactcc tatccggaag gggcatacga gttccgcgcg accggctacg acctggccgg
     1081 caacgccacc agctcgaccg tgcgcagcag cggcgcggcg atggtcctgc tcaacccggt
     1141 caaggtgcgg acccaactgc ggttcggctt cggcggccgg cgcctggtct ggcagcgctg
     1201 cgtgcgcaag ggcgagagcc ggcgctgccg gcgcgaggtg ctcgagtcgt tcgcgcgccg
     1261 gcccgcgagc cgcctcgtcc cctacgggcg cggcctgcag gtcggcggcc ggttgctgac
     1321 cgcgtccggc gctcccctgg ccggcgccac cgtcgagctg gtcgaggcgt tcgaccccgg
     1381 cgccggtatt cagagccgcg tgaccagcct gcgcaccgac gccgaaggcg gcttcctcgc
     1441 ccgcctctcc cccgggccga gtcggcggat cgaagcgcgc ttcggcggcg accgcctgct
     1501 cacccgggcc accagccgcg atctgcggct cggcgtccaa accctggtcc gtctgcgagc
     1561 ctcgaccgcc ggagccgaga tcggcggcgc cccggtcgtg ttcagcggcc gggtcggggc
     1621 cagcgaagca acgatccccg tctacggccg cccggtccag ctgcagttcc gcctccccgg
     1681 ctcgccgtgg accgagttcc gcacggtcca gaccgacgcc cacggccgct tccgcttccc
     1741 ctacgcgttc accgacgacg acagccgcgg ggtccgcttc ctcttccgcg cctacgcccc
     1801 agcccagccc ggctggccct acgaaccggc ggcctcccgc ccggtcgccg tcaccggccg
     1861 ctaggcccct agagcgttca gaaccaagcg gggcgacgac gaaaaaaggc ccgcttgctg
     1921 gttgcaagcg ggcctttttg agggggcgca tccgcgccgg ttatggacgc tctagcgcag
     1981 cgcggcgagc tggtcggtga cggaaccgac caggtccgag gcgcggctgg tctgctcgtc
     2041 gatcgccttg cggacgcgcg actcgacctc ggcgcggttg cgcttcagcg tggtctcaac
     2101 cgtgcgctgc cgtttgcggg cttcgcgctc gacccggttg cgggttttgc gggcttcggt
     2161 ggtcgctttg cggcgggcgc tggtgccgcg gcgctcagtc cgtttgagcg atttgcgcag
     2221 ctgggactgg tagctcttga gctgtttctc ggcgctgctg cggccggtcc acggctcgac
     2281 cagatcggag acgcggtcgc tgacgctgag cacgatgccg accgggatgt cgacggcggt
     2341 ctcggcggcg gtcaggacct ggttcttctc ggcgacggcg gcgcgtttgg ttgctttcac
     2401 gctcgtttta gccgattttt tcgcttcagt ggtggcgcgt ttggcctgcg ttttggcttt
     2461 gggactgctt ttctctgcca tgaaagtgag ggaatccctt cgagtaatcg gttggctgcc
     2521 tcggatttcc gagtgtagcg aacatatgtt ccacgaacag acgttcctaa acacttttag
     2581 gcgtggagtt acacgagtgc cgcaaaaagc gccctctttt tgggaagtca gtgcctgaag
     2641 cgggtcaggc aatccgtgcc tggagggaac ttcaggcgga ctgggcggtc cgcaccgtcg
     2701 ccagcccggc ggcaccgctg gcggcggcca tcggatagcg cgcatccagg tcgagcggcg
     2761 cctcgcccag cgccagctca ccaaaggcga cctccccacc ggcctcgcgc acgaacagct
     2821 gcgcggcggc ggcgtcgacc gaacgacagg ggcgcaggct gaacatcgcg tcgaagcgac
     2881 cgctgccgac gtaggcggcg gtgatcgcga tcgagccgac cacgcgcagc cggtaggcct
     2941 tgccgtcgag cgccgccagc gccggctgcg accactgcgg ctccgccgac tccagcccga
     3001 ccacctcgag ccgctcgccc tcggcggcga cctgggcggc ggactcggcg acgctgccgt
     3061 cgacatggcc gcgccgcacg ctcgccccct ccccgcggcg cgcgacgaac tcctcgtcgg
     3121 cgccgaaatc gtggacgaag ccgaactcga cgtcggccat cgaggaaccg gatgcaacgg
     3181 cgatgctgag gctgtgtgac gggatcgtgc ggcgggcgtt gagcgagccg tcga
//
LOCUS       DASRIG010000219         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2183795, whole genome shotgun sequence.
ACCESSION   DASRIG010000219 DASRIG010000000
VERSION     DASRIG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183795"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1375
                     /locus_tag="VFB52_06090"
     CDS             257..1375
                     /locus_tag="VFB52_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO05938.1"
                     /translation="MPGVATGQDSPDPKTRRDELSNAIEGISAEEVTALNELQGITAH
                     RQDLEAKVAALDRQIAEASQRVAAAEAEIADIQSQVETVQRDIDRIAAEIEASKGKFN
                     ASALALYKGGGNGTTNFTLLSTSGGAHEVIAGSKYLGENSRRFERELQRQGSLKVQLD
                     DAQTDLRKEQAKAEDAQRVAAEERDHVTQLRSQADGERDQVAAAEQQEQQAIEGLRAR
                     KADFEAQYDAVQAQISASVSRGNPTPGNHRFSWPVNGPITSPFGYRNDPVLGGNRLHA
                     GVDIGASSGTPIKAAGDGVVIMAGWNGGYGNFTLIDHGGGLATGYGHQSSIGVRVGQH
                     VSTGEVIGNVGSTGASTGPHLHWEVRVNGTPVDPMGWV"
     gene            complement(1439..2074)
                     /locus_tag="VFB52_06095"
     CDS             complement(1439..2074)
                     /locus_tag="VFB52_06095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO05939.1"
                     /translation="MSLRLGDTAPDFTAETTEGTLSFYDYLGDSWGMLFSHPKDFTPV
                     CTTELGAFARRRSEFDKRNVKLIGISVDPLDSHKGWSKDIEETQGAAPNYPLIADPDR
                     KVADLYDMIHPNASDTVTVRSVFIIGPDKKIKLIITYPQSAGRNVDELLRVIDSLQLT
                     AGYSVSTPVDWKEGEDVIIVPAVSDEDAKQKFPKGFKTIKPYLRVTPQPNK"
     gene            2208..>2394
                     /locus_tag="VFB52_06100"
     CDS             2208..>2394
                     /locus_tag="VFB52_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HZO05940.1"
                     /translation="MAGYSGTPLPKKLGIKEGARVALIGAPNGFDRTLGNLPPGVEVR
                     RRARGSFDVIVVFVTRRA"
BASE COUNT          378 a          786 c          847 g          383 t
ORIGIN      
        1 tcggggccgg ggtcggcctg cgccgcttcc tgaacgtctg accgccggcc acgcgcccgg
       61 gatccgccgg gaggggagtg ggccgtactt ccgagaggcc tgccggcggt gtgtcgcgcc
      121 cgttgctctc ggggcaagtg cgcatttcgg gccggatttg ttgcttgtgt gactcatgtt
      181 gcgtacgctc agaaccctga aatcccatcg ttttctgctc tgtctcgtcg cggccgcgct
      241 cctgctcggt ctcgtcatgc ccggcgtcgc caccggtcag gactccccgg atcccaagac
      301 ccgtcgcgac gagctctcga acgcgatcga gggcatctcg gccgaggagg tcacggcact
      361 caacgagctg caggggatca ccgcgcaccg ccaggacctc gaggcgaagg tggcggcgct
      421 cgaccgacag atcgccgagg ccagccagcg agtcgcggcc gcagaagccg agatcgccga
      481 catccagtcc caggtcgaga cggtgcagcg cgacatcgac cggatcgcgg ccgagatcga
      541 agcctcgaag ggcaagttca acgcgtcggc gctcgcgttg tacaagggcg gcggaaacgg
      601 cacgaccaac ttcacgctcc tctcgacgag cggcggcgcc cacgaggtga tcgccgggag
      661 caagtacctc ggcgagaact cgcgtcgctt cgagcgggag ctgcagcgcc agggcagtct
      721 caaagtccag ctcgacgacg cccagaccga cctccgcaag gagcaggcga aggcggagga
      781 cgcccaacgc gtcgcggccg aggagcggga ccacgtcacc caactccgga gtcaggccga
      841 cggcgagcgg gaccaggtgg cggcggccga gcagcaggag cagcaggcca tcgagggcct
      901 ccgtgcccgc aaggccgact tcgaggcgca gtacgacgcg gtccaggccc agatctcggc
      961 cagcgtgagc cgcggcaacc cgacgccggg gaaccaccgt ttcagctggc ccgtgaacgg
     1021 tcccatcacc agcccgttcg gctatcgaaa cgatccggtg ctgggcggga accgccttca
     1081 cgccggtgtc gacatcgggg cctcctcggg cacgccgatc aaggcggccg gggacggcgt
     1141 cgtcatcatg gccggctgga acggcggata cggcaacttc acgctgatcg accacggcgg
     1201 cgggctcgcc acggggtacg gccaccagtc gagcatcggc gtccgcgtcg gccagcacgt
     1261 gagcaccggc gaggtcatcg gcaacgtcgg ctccaccggc gcgtcgaccg gcccgcacct
     1321 ccactgggag gtgcgcgtca acggcactcc cgtcgacccg atgggctggg tctagccgcc
     1381 gcacccgccg ttgggtcgaa accccgctcg gcacggggtt tccgtcccga cgtgggagtt
     1441 acttgttcgg ctggggggtg acccgcaggt agggcttgat ggtcttgaag cccttgggga
     1501 acttctgctt cgcgtcctcg tccgagacgg ccgggacgat gatcacgtcc tcgccttcct
     1561 tccagtcgac cggcgtcgag acgctgtagc cggcggtcag ctggagcgag tcgatcacgc
     1621 gcaggagctc gtcgacgttg cgcccggcac tctgcgggta cgtgatgatc agcttgatct
     1681 tcttgtccgg gccgatgatg aacaccgagc gtacggtcac cgtgtcgctg gcgttggggt
     1741 ggatcatgtc gtagaggtcg gccaccttgc ggtccgggtc ggcgatcagc ggatagttcg
     1801 gggcggcgcc ctgcgtctcc tcgatgtcct tcgaccagcc cttgtgcgag tcgagggggt
     1861 cgacgctgat cccgatcagc ttgacgttgc gcttgtcgaa ctcagacctc cgccgtgcga
     1921 aggcacccag ctcggtggtg cacacgggcg tgaagtcctt ggggtgcgag aagagcatcc
     1981 cccagctgtc gccgaggtag tcgtagaagc tcagcgtccc ttcggtggtc tcggcggtga
     2041 agtcgggagc ggtgtctccc aggcgaagtg acatggctgc ctctcctgtg gctcgtgctc
     2101 tggctcgttt ctctcaaggt atcagcggcc ggcgaaaagt cgagcaaaaa ggtcggaata
     2161 tttggggccc ctccgtcgcg gagcggcggc ggtgggaagc tgccccgatg gcggggtact
     2221 ccggcacgcc gttgccgaag aagctgggga tcaaggaggg cgcgcgggtc gccctgatcg
     2281 gcgcgccgaa cggcttcgat cgcacgctcg gaaacctccc gccgggggtc gaggtgcgta
     2341 ggcgggcgcg gggctcgttc gacgtgatcg tcgtgttcgt cacccgacgg gccg
//
LOCUS       DASRIG010000220         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1161258, whole genome shotgun sequence.
ACCESSION   DASRIG010000220 DASRIG010000000
VERSION     DASRIG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161258"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFB52_06105"
     CDS             complement(<1..449)
                     /locus_tag="VFB52_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO05941.1"
                     /translation="MSATPKKSKLIADLITAFRASGNQDSAFENLAAERLGINRTDLH
                     CLNAIENAGGLSAGELATEAGLTSGAVTGVVDRLERAGFARRVPDPADRRRVKLEVTP
                     EFYARAAEIWGPMAADWESTLAERFTAAELARITEFLRLTNEVGKRHL"
     gene            538..906
                     /locus_tag="VFB52_06110"
     CDS             538..906
                     /locus_tag="VFB52_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HZO05942.1"
                     /translation="MDWKLELVQVPVSDVDRAKAFYADQLGFVAEHDHRVSDELRFVQ
                     LTPPGSACSISLTTGAHEMAPGSIEGLQLVVADADAARAELLERGVEASEVQEFPWGR
                     FVFFADPDGNRWAVQQITQP"
     gene            965..2275
                     /locus_tag="VFB52_06115"
     CDS             965..2275
                     /locus_tag="VFB52_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZO05943.1"
                     /translation="MLRRGALAVFSGLVLLALPAAASAATQVSVIHPEGLDVVFASDD
                     GGDAEINATRSLESGTMQPYALVQSEGGAVAGSGCEQIASTLVACAGSFDAVLVEGGG
                     GNDEISLNLIYESLPPLRGEALGGPGNDTLEATFDNRPIPQPETALVGEAGNDTLVGG
                     YGLDELFGGEGDDFLSAGGESAVPNAADLLDGGAGVDTVSDADYNRGLDDDVAVTLDG
                     IANDGEPGEGDNVTAVEKLRVVGNRVTLIGSEAAEQLSVEANASTIDGKAGLDTLTGG
                     AGSDAIEARDGISEPVDCGAGSDSAQLDAGDRASGCESINGALVGPLGFGEKTLVTLK
                     LASKRIPAAGPVKVRVANANPFRVSGSLSGRVGKAKLKAKAFSVGAGAKKTVALKLPA
                     TLREALAETGKLAVKLSARVRDPAGNTRTVKRQVTPKLKQPQGR"
     gene            complement(2282..3127)
                     /locus_tag="VFB52_06120"
     CDS             complement(2282..3127)
                     /locus_tag="VFB52_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05944.1"
                     /translation="MKSRSPLVLLFVLVALLPAAPASAAFGEGDGMFDDAAGTRTLVW
                     HNGELREEIHVVATARRESEPWYPCGGYVYPGGTLDIRMVPLLGPVGGFFDANGKLLY
                     NCGPAPSFVPVLPQWLEVEYTSCKAKDLGGSKADTDYTNTTPMVVGGRTVEEQRVDDT
                     DLGYTFLGDDAPEKFCITEFVTGSRPAKWCSPGGVENEFCPREYGSGNIPLQEGTLRY
                     LPTPTPGCAMAAKSERKIRKELKQLAARLRTRTRGRWWLESRFDSRREFYDYVAKPRA
                     VANCR"
     gene            complement(3157..>3286)
                     /locus_tag="VFB52_06125"
     CDS             complement(3157..>3286)
                     /locus_tag="VFB52_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO05945.1"
                     /translation="ATNREVATALFLSPRTVEHHLRSAYRKLGVRSRTELAARRGG"
BASE COUNT          511 a         1101 c         1189 g          485 t
ORIGIN      
        1 aggtgccgct tcccgacctc gttcgtcagc cgcaggaact ccgtgatcct tgccagctcc
       61 gcggccgtga accgctccgc cagcgtcgac tcccagtcgg ccgccatcgg cccccagatc
      121 tccgccgctc gggcgtagaa ctccggcgtc acctcgagct tcacccgccg gcggtccgcc
      181 gggtcgggga cgcggcgggc gaagccggcg cgctcgaggc ggtcgacgac gccggtcacg
      241 gcgccgctgg tcaggcccgc ctcggtggcg agctcgccgg cgctgaggcc gccggcgttc
      301 tcgatcgcgt tcaggcagtg caggtcggtg cggttgatcc cgagtcgctc ggcggcgagg
      361 ttttcaaagg cgctgtcctg gttgccgctg gcgcggaagg cggtgatcag gtcggcgatc
      421 agtttgctct ttttcggggt tgcagacata tctcttagtt gctaagatac ttggggacta
      481 aggaacttac cgggtgagat aataataggc gcccgggcaa ccgaaaggga gtcgcaaatg
      541 gactggaagc tggagttggt gcaggtgccg gtatccgacg tcgaccgggc aaaggccttc
      601 tacgccgacc agctcggctt cgtcgccgaa cacgaccacc gggtcagcga cgagctgcgt
      661 ttcgtgcagc tcaccccgcc cggctcggcc tgctcgatct cgctgaccac cggcgcgcac
      721 gagatggcgc cgggcagcat cgagggcctg cagctggtcg tcgccgacgc tgacgcggcg
      781 cgggcggagc tgctcgagcg cggggtcgag gcaagcgagg tgcaggagtt cccctggggc
      841 cgcttcgtct tcttcgccga cccggacggc aaccgctggg cggtgcagca gatcactcag
      901 ccctgagcag ggaaccggcg ggcgcggttt gacgccgcgc ccgcggtcga ggatgatcgg
      961 cgcgatgctg cgcaggggcg cactcgcggt cttctcgggg ttggtgctgc tggcgctgcc
     1021 cgcggcggca agcgcggcga cccaggtgag cgtgatccac cccgaagggc tcgacgtcgt
     1081 gttcgccagc gacgacggcg gcgacgctga gatcaacgcc acgcgctcac tcgaatcggg
     1141 cacgatgcag ccctacgccc tagtgcagag cgagggtgga gcggtggcgg gcagtggctg
     1201 cgaacagatc gcgagcacgc tggtcgcctg cgccggcagc ttcgacgccg tgctcgtgga
     1261 aggcggcggc ggcaacgacg aaatctcgct gaacctgatc tacgaatcgc tgcccccgct
     1321 gcgcggtgag gcgctcggcg gtcccggcaa cgacacgctc gaagcaacct tcgacaatcg
     1381 cccgatccct cagcccgaaa ccgccctcgt cggcgaggcc ggcaacgaca cgctggtcgg
     1441 cggttacggg ctcgacgaac ttttcggcgg ggaaggggat gacttcctca gcgcgggtgg
     1501 ggagtcggcc gttcccaacg cggccgacct gctcgacggc ggcgccggcg tcgacacggt
     1561 ctccgacgcc gactacaacc gcggcctcga cgacgacgtc gcggtcacgc tcgacgggat
     1621 cgccaacgac ggcgagcccg gcgagggcga caacgtgacc gcggtcgaga aactgcgggt
     1681 agtcggcaac cgggtgacct tgatcggcag cgaggcggcc gagcagctct cagtggaagc
     1741 gaacgcgagc acgatcgacg gcaaggccgg gctcgacacg cttaccggcg gcgccggcag
     1801 cgacgcgatc gaggcccgcg acgggatctc cgagccggtc gactgcggcg ctggcagcga
     1861 cagcgcgcaa ctcgacgccg gcgaccgggc cagcggctgc gagtcgatca acggggccct
     1921 ggtcggcccg ctcggtttcg gtgagaagac gctggtgacg ctgaagctgg cgagcaagcg
     1981 gatccccgcg gcggggccgg tcaaggtccg ggtggcgaac gccaacccgt tccgggtcag
     2041 cggctcgctt tccggtcgag tcggcaaagc gaagctgaaa gcgaaggcat tcagcgtcgg
     2101 cgccggcgcg aagaagaccg tcgcgctgaa gctgccggcg actctgcgcg aggcgctggc
     2161 ggagaccggc aagctcgcgg tgaagctgag cgcgcgggtc cgcgacccgg ccggcaacac
     2221 gcgcacggtc aaacgccagg tcaccccgaa gctgaagcag ccgcagggcc gctgaccgcg
     2281 ctcagcggca gttggccacg gcccgcggct tcgccacgta gtcgtagaac tcgcgccgcg
     2341 agtcgaagcg gctctccagc caccagcggc cgcgggtccg ggtgcgcagc cgcgcggcga
     2401 gctgcttgag ttccttcctt atcttccgct ccgacttcgc ggccatcgcg cagccggggg
     2461 tcggcgtcgg caggtagcgc agcgtccctt cctgcagggg gatgttgccc gagccgtatt
     2521 cgcgtgggca gaactcgttc tcgaccccac cgggggagca ccacttcgcc ggtcgcgagc
     2581 cggtgacgaa ttcggtgatg cagaactttt ccggcgcgtc gtcgccaagg aaggtgtagc
     2641 ccaggtcggt gtcgtctaca cgctgttctt cgacggtccg cccgccgacc accatcgggg
     2701 tcgtgttcgt gtagtcggtg tcggctttgg atccccctag gtccttcgcc ttgcacgagg
     2761 tgtattcgac ctccagccac tgcggcagca ccggaacgaa gctcggcgcc gggccgcagt
     2821 tgtagagcag tttgccgttg gcgtcgaaga agccgcccac cgggccgagc agaggcacca
     2881 tgcggatgtc cagcgtcccg cctgggtaga cgtagccccc gcaggggtac cagggctccg
     2941 attcccgcct cgccgtggcg accacgtgga tctcttccct cagttcgccg ttgtgccaga
     3001 ccagcgtcct ggtgcccgcg gcgtcgtcga acatgccgtc gccttcgccg aacgccgcgc
     3061 tcgccggggc ggccgggagc agggcaacga ggacgaacag cagcacgagc ggggaccgag
     3121 acttcaccgc gacgattcta gagcgatccg ggggtctcaa ccgccgcggc gggcggcgag
     3181 ctcggtgcgc gagcgcacgc cgagcttgcg gtaggcggaa cgcaggtggt gctcgaccgt
     3241 gcgcggggag agaaacagcg cggtggcgac ctcgcggttg gtggcg
//
LOCUS       DASRIG010000221         7078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1675006, whole genome shotgun sequence.
ACCESSION   DASRIG010000221 DASRIG010000000
VERSION     DASRIG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675006"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFB52_06130"
     CDS             <1..1063
                     /locus_tag="VFB52_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZO05946.1"
                     /translation="QVSAQAREELLQLNSEARKLDAQTAEAALDKRQAEIKALAERIA
                     RGQEKIEAEVRQRGETDLQTRTLIQQLGQTVGNLNSETAGLKKALRQPQTRGQWGELQ
                     LRRCIEIAGMTEHVDFELQETLRTDDGRLRPDARFLLPEGRSFVADSKVPLDAFLDAQ
                     EAEVESERRVHMERHARQAREHVRALSSKDYQGQFKAGEMPDLVICFIPNEPALHAAF
                     TADPELFDYALERNVLLVSPTSLIGLLRTMELGWRQERMVAEAAEIANAASTLHSRFS
                     KFISDFDTVGQRLGSATKAYNSAVGSMEGRLLPQLRKVEGLGIATGKEIEEPQPVELQ
                     VRAISAPELREAPDQADAA"
     gene            1166..2392
                     /gene="ccrA"
                     /locus_tag="VFB52_06135"
     CDS             1166..2392
                     /gene="ccrA"
                     /locus_tag="VFB52_06135"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HZO05947.1"
                     /translation="MEPGTLPESMAAWVIRQEREGEPKDAFQLEQIEVPEPGAFEVIV
                     RVMAAGVNFNNVWASLGKPVSVFGYGDHPEYGHHIGGSDASGVVWKVGEGVTRWQPGD
                     EVVIHCNQASYEDVEVHGLDPLAAPSQRIWGYETTWGSFAQFTKVQAQQLLPKPKNLS
                     WVDSSAYGLTYFTAYRMLMDQCKLQAGHNVLIWGAAGGLGVFAVQLCKAAGANAVGVV
                     SSDEKGELVKQLGAVGYINRNEFSEMMRTKDNLTDPAADKARFKASRAFGNKVSEILG
                     DAPDIVFEHVGQATFPTSVLVVKPFGKVVICGATSGFQLDFDVRYLWMRQKQIIGSHF
                     ANAYECMRANQLMAEGKVRPVLSETMGFEGVPTAHQLMHENKHLGKIAILVGAESEDE
                     GKTEDGPGAIRAEVGA"
     gene            2428..2721
                     /locus_tag="VFB52_06140"
     CDS             2428..2721
                     /locus_tag="VFB52_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05948.1"
                     /translation="MAEVNRLDWHIAPFRADRWLDLWEPAAAKMPAYGATSWSLTRAK
                     DDPLAFQQTSIWEQRSDFERYWFSEEIEKVREGIIGWYDLPLLPTWHTLVLAE"
     gene            complement(2723..5347)
                     /locus_tag="VFB52_06145"
     CDS             complement(2723..5347)
                     /locus_tag="VFB52_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZO05949.1"
                     /translation="MTKLLLGALALLTFVLAAAASAGAATLPSGFRDDLYLTTLGQPT
                     SFAFAPDGTVFVAEKTGRIMVYDDIDEDEKKLFADLRKPVYDNGDRGLLSIALDPDFP
                     TKPFVYALYTFDHVLGVDPPGKFPHWGEGPGYVGDPCPLPEGADADSCPVSGRLVRLT
                     AESTPGGYKATQSGGEADQKPLLEDWCQQFSSHSIGDLQFAADGSLYVSGGEGANYNV
                     PDYGQFGWPQKNMCADPPGGTIGSVLTPPSAEGGALRSQDLLTPATLGDPTGLSGTVA
                     RVDPETGAGLPGNPLFDSFDANERRIVAFGFRNPFRIALDAQNGDLYIANVGWGTYEE
                     IDRVPELPDGEPPDSGWPCIEGPRWHSSYENVSLNLCNQLYADPDSTIEPFFFYRHGT
                     DVLPGDGCKTENGAALAAMDVYRGGKFPARYEGALFFADTVRGCIWVMYPDEHDGRPD
                     PETTELFLGEGTNFPGIDIHTGPEGNLYYLTFDANDGEGSLHRVTYDPASPIARIEAS
                     PEWGPVDPDPLEVDFDASGSEDPAGGTLEYEWDLNGNGSFETDTGTSPVASKTYSDSE
                     NVKVSVRVDNGTKESVARITVYPGDTPPVPEIDTPSAAHTWRVGEPIYFEGDAADEEE
                     GGGEIDDSNLRWKTRLLHCPAACHAHPLQTFPGTDSGEFIAPDHDYPAKIELSLTATD
                     SRGLSATTSIQLLPDTVDLRIVSDPAGLPLGAGPSSEVAPYTLRAIIGSNVVLSAPPK
                     ALIDGVEQPWLGWSDGGARVHTIVASQSGTYTATYQHAEPPPPPPVNRPNTKLAKKPE
                     KQTFSRSARFVFSSSLAGSSFRCKLDQNPYKACRSPRVYRNLALGKHVLRVQATAADG
                     TIEAQPALYRWRVLLPRR"
     gene            complement(5392..5613)
                     /locus_tag="VFB52_06150"
     CDS             complement(5392..5613)
                     /locus_tag="VFB52_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HZO05950.1"
                     /translation="MIVRNDNHNTFEHVAQTLARVLPGVTLEQGHRIADTIHNKGQAI
                     VWSGPRELAEHYWAQLDGAGLTMAPLERS"
     gene            5694..>7078
                     /locus_tag="VFB52_06155"
     CDS             5694..>7078
                     /locus_tag="VFB52_06155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZO05951.1"
                     /translation="MHPISRPADSRFPYLPGIDALRALAVLAVFFYHAGVNWMPGGFL
                     GVDLFFVISGYLITSLLLREFRNGGHVKLGRFWLRRARRLLPAVGVMIAVTMVVGAIV
                     EPDRVDQMRGDAIASLAYFANWHFVYTDVSYFDQFQRPSLFTHLWSLSVEEQFYLFWP
                     LVFAAGMKLFGRGKLLLGVAAGALASVALAWILFDPGADASRIYYGTDTHAVGLLAGV
                     ALALVWSPIELRRRKTGPLVGPILDVVGVFALAFIVLSFVNVNDYDLGLWHGGYLWLA
                     LVTTLLIAVIVHPAARLGGLLGQKPVLWLGLRSYSFYLWHWPVLVLTRPGVDVDLPTE
                     VLVPLQLALVLLLSDLSYRYVELPFRGQGEVKLSAGWLRVARPALVAGVLAVVLFVGW
                     SGLFSSNEDRLDAASAAQRQTKAVVEAQPERVKPEGEQSEPGPDHPPGAYEPPQTYQE
                     PRVPTATNAMPP"
BASE COUNT         1070 a         2413 c         2452 g         1143 t
ORIGIN      
        1 ccaggtcagc gcccaggcgc gcgaagagct gctgcagctg aacagcgagg cgcgcaaact
       61 cgacgcgcag accgccgagg cggcgctcga caagcgccag gcggagatca aagcgctggc
      121 cgagcggatc gcccgcggcc aggagaagat cgaggcggag gtccgccagc gcggcgaaac
      181 cgacctgcag acccgcaccc tgatccagca gctcggccag accgtcggca acctcaactc
      241 ggagaccgcc ggcctgaaga aggcgctgcg ccagccgcag acccgcggcc agtggggcga
      301 gctgcagctg cgccgctgca tcgagatcgc cgggatgacc gagcacgtcg acttcgagct
      361 gcaggagacg ctgcgcaccg acgacggccg cctgcgcccg gacgcgcgct tcctgctgcc
      421 cgagggccgc agcttcgtcg ccgactcgaa ggtgccgctc gatgccttcc tcgacgccca
      481 ggaggccgag gtcgagtccg agcgccgcgt gcacatggaa cggcacgcgc gacaggcgcg
      541 cgagcacgtg cgggcactca gctcgaagga ctaccagggc cagttcaaag ccggcgagat
      601 gcccgacctg gtgatctgct tcatccccaa cgagcccgcc ctgcacgccg ccttcaccgc
      661 cgacccggag ctgttcgact acgcgctgga gcgcaacgtg ctgctggtca gcccgacctc
      721 gttgatcggc ctgctgcgga cgatggagct gggctggcgc caggagcgga tggtcgccga
      781 ggcggccgag atcgccaacg cggcgagcac cctgcactcg cgcttctcga agttcatcag
      841 cgacttcgac acggtcggcc agcgcctcgg ctcggcgacc aaggcctaca actcggcggt
      901 cggctcgatg gagggccggc tgctgcctca gctgcgcaag gtcgagggcc tcggcatcgc
      961 caccggcaag gagatcgagg agccgcaacc ggtcgagctc caggtccgcg cgatctcggc
     1021 gccggagctg cgcgaggctc ctgaccaggc cgacgccgcc tagcggcggc ctcgcttccc
     1081 cccagctagg attgccgcga aagcgggaga ttcaaggacc ggaggaattt tggcggtaga
     1141 tacgagcgca gtcgcaaagc tggacgtgga gccgggcacg ctgcccgaat cgatggcggc
     1201 ctgggtgatc cggcaggagc gcgagggcga gcccaaagac gccttccagc tcgagcagat
     1261 cgaagtcccc gagcccggcg ccttcgaggt gatcgtccgg gtgatggcgg ccggcgtcaa
     1321 cttcaacaac gtctgggctt cgctcggcaa accggtctcg gtcttcggct acggcgacca
     1381 tcccgagtac ggccaccaca tcggcggctc cgacgcctct ggggtcgtct ggaaagtcgg
     1441 cgagggcgtg acccgctggc agcccggcga cgaggtcgtg atccactgca accaggcctc
     1501 ctacgaggac gtcgaggtgc acgggctcga cccgctcgcg gctccgagcc agcggatctg
     1561 gggctacgag accacctggg gctcgttcgc ccagttcacc aaggtgcagg cgcagcagct
     1621 gctgccgaag ccgaaaaacc tctcctgggt cgactcctcc gcttacgggc tcacctactt
     1681 caccgcctac cggatgctga tggaccagtg caagctgcag gccggccaca acgtgctgat
     1741 ctggggcgcc gccggcggcc tcggcgtctt cgccgtgcag ctctgcaaag cggccggggc
     1801 gaacgcggtc ggcgtcgtct cctccgacga gaagggcgaa ctggtcaagc agctcggcgc
     1861 ggtcggctac atcaaccgca acgagttcag cgagatgatg cggaccaagg acaacctcac
     1921 cgacccggcc gccgacaagg cccgcttcaa ggcctcgcgc gccttcggca acaaggtctc
     1981 ggagatcctc ggcgacgccc ccgacatcgt cttcgagcac gtcggccagg cgaccttccc
     2041 gacctcggtc ctggtcgtca agccgttcgg caaagtcgtg atctgcggcg ccacctcggg
     2101 cttccagctc gacttcgacg tgcgctacct gtggatgcgc cagaagcaga tcatcggctc
     2161 ccacttcgcc aacgcctacg agtgcatgcg ggcgaaccag ctgatggcgg agggcaaggt
     2221 gcggcccgtg ctctcggaaa cgatgggctt cgagggcgtc cccaccgccc accagctgat
     2281 gcacgaaaac aagcacctcg gcaagatcgc gatcctggtc ggcgccgagt ccgaggacga
     2341 gggcaagacc gaggacggcc cgggcgcgat ccgcgccgag gtcggggcct aaccaaccac
     2401 tgcaagcgcg acgaaagagg taaccgaatg gctgaggtca acaggctcga ctggcacatc
     2461 gcccccttcc gggcggaccg ctggctcgac ctctgggaac cggcggcggc gaagatgccc
     2521 gcctacgggg ccaccagctg gtcgctgacc cgcgccaagg acgacccgct ggccttccag
     2581 cagacctcga tctgggagca gcgctcggac ttcgaacggt attggttctc cgaggagatc
     2641 gagaaggtcc gcgaggggat catcggctgg tacgacctgc cgctgctgcc gacctggcac
     2701 acgctcgttc tcgccgagta gctcagcgcc gcggcagcag gacccgccag cggtagagcg
     2761 ccggctgcgc ctcgatcgtc ccgtcggcgg cggtcgcctg cacccgcagt acgtgcttgc
     2821 caagcgcgag gttgcggtag acgcgcggtg agcggcaggc cttgtagggg ttctggtcga
     2881 gcttgcagcg gaagctggag ccggccaggc tggagctgaa cacgaagcgc gcgctgcggc
     2941 tgaaggtctg cttctccggc ttcttggcaa gtttcgtgtt cggccggttg accggcggcg
     3001 gaggcggcgg ctcggcgtgc tggtaggtcg ccgtgtacgt gccggactgg ctggcgacga
     3061 tcgtgtgcac ccgggcgccg ccgtccgacc agccgagcca gggctgctcg accccgtcga
     3121 tcagcgcctt cggcggcgcc gagaggacga cgttcgagcc gatgatcgcg cgcagcgtgt
     3181 acggcgccac ctcgctgctc ggccccgcgc ccaggggcag gccggccggg tcggagacga
     3241 tccgcaggtc gacggtgtcc ggcagcagct ggatcgaggt ggtcgccgag agcccgcgtg
     3301 agtcggtcgc ggtcaggctc agttcgattt tcgccgggta gtcatggtcg ggagcgatga
     3361 actcgcccga atcggtcccg gggaaggtct gcagcgggtg cgcatggcag gccgccgggc
     3421 agtgcagcag ccgcgtcttc cagcgcaggt tcgagtcgtc gatctcccca cccccctcct
     3481 cctcgtcggc ggcgtcgccc tcgaagtaga tcggttcgcc tacccgccag gtgtgggccg
     3541 cgctcggggt gtcgatctcc ggcaccggcg gcgtgtcgcc ggggtagacg gtgatccggg
     3601 cgacgctttc tttggtcccg ttgtcgaccc gcaccgagac cttcacgttt tcgctgtccg
     3661 agtacgtttt gctcgccacc ggcgaggtgc ccgtgtcggt ctcgaagctg ccgttgccgt
     3721 tcaggtccca ttcgtattcg agcgtgccgc ccgcggggtc ttcggagccg ctggcgtcga
     3781 agtcgacctc gagcgggtcg gggtctaccg ggccccattc cgggctcgcc tcgatccgcg
     3841 caatcggcga tgcggggtcg taggtgaccc ggtgcagcga gccttcgccg tcgttggcgt
     3901 cgaaggtcag gtagtagaga ttgccttccg gcccggtgtg gatgtcgatg cccggaaagt
     3961 tcgtgccttc gccgaggaac agctcggtcg tctccgggtc cggccgcccg tcgtgctcat
     4021 ccgggtacat cacccagatg cagccgcgca cggtgtcggc aaagaacaac gcgccttcgt
     4081 agcgggccgg gaatttgccg ccgcggtaga cgtccatcgc cgccagcgcg gcgccgtttt
     4141 cggtcttaca gccgtcgccg ggcagcacgt cggtcccgtg gcggtagaag aagaacggtt
     4201 cgatggtcga atcgggatcg gcgtagagct gattgcagag gttgagactg acgttctcgt
     4261 aggagctgtg ccagcgcggc ccttcgatgc agggccagcc cgagtcgggc ggctcgccgt
     4321 ccggcagctc cggaacccgg tcgatctcct cgtaggtgcc ccagccgacg ttggcgatgt
     4381 agagatcgcc gttctgggcg tcgagcgcaa tccggaacgg gttgcgaaag ccgaaggcaa
     4441 cgattcgccg ctcgttggcg tcgaagctgt cgaacagcgg gttgccgggg agcccggcgc
     4501 cggtttccgg gtcgactcgg gcgaccgtgc cggagagtcc ggtcgggtcc ccgagggtgg
     4561 ccggggtgag caggtcctgg gagcgcagag cgccgccttc ggcgctcggg ggcgtcagca
     4621 ccgacccgat ggttcccccc ggcgggtccg cgcacatgtt tttctgcggc cagccgaact
     4681 ggccgtagtc gggcacgttg tagttggcgc cctcgccacc gctgacgtag agcgagccgt
     4741 cggcggcgaa ctggaggtcg ccgatcgagt gcgaggaaaa ctgctggcac cagtcttcga
     4801 gcaacggttt ctggtcggct tcgccacccg actgggtcgc tttgtagccg cctggggtcg
     4861 attcggccgt cagccggacc aggcggccgg agaccggaca ggaatcggcg tccgcacctt
     4921 cgggcagcgg gcaggggtcg ccgacgtagc cggggccttc gccccaatgg gggaatttgc
     4981 ccggcggatc gaccccgagc acgtggtcga aggtgtagag cgcgtagacg aacggtttgg
     5041 tcgggaagtc ggggtcgagc gcgatcgaga gcaggccgcg gtcgccgttg tcgtagaccg
     5101 gtttgcgcag gtcggcgaag agtttttttt cgtcctcgtc gatgtcgtcg tagaccatga
     5161 tccggccggt tttctcggcc acgaagacgg tcccgtcggg ggcgaaggcg aacgaggtcg
     5221 gctgcccgag cgtggtcagg tagaggtcgt cgcggaagcc cgagggcagg gtcgcggcgc
     5281 cggcgctcgc ggccgcggcg aggacgaacg tgagcagggc gagcgctccc agcaacagct
     5341 tggtcacggg atgagaaacc gcgacgtatc gcctgagttg cggttgcgcg ttcaagagcg
     5401 ctcgagcggc gccatcgtca acccggcgcc gtccagctgc gcccagtagt gctcagccag
     5461 ctcccggggc ccggaccaga cgatcgcctg gcccttgttg tggatcgtgt cggcgatccg
     5521 gtgaccctgc tcgagggtga ctccgggaag gacgcgcgcg agcgtctgag cgacgtgctc
     5581 gaaggtgttg tggttgtcgt ttcggacgat cacgcgccag tccccgccga gtcctgaatc
     5641 aggtcctgca ctgcgtggcc gctcgaccgt ggaagcggac atcgcccgcc attatgcatc
     5701 ccatcagccg accagctgac agtcgcttcc cctatctacc cggcatcgac gccctgcggg
     5761 cgctcgcagt gctggcagtt ttcttctacc acgccggtgt caattggatg ccaggcggtt
     5821 tcctcggcgt cgatctcttc ttcgtgatca gcggctacct gatcacctcg ctgctcctgc
     5881 gtgagttccg caacggcggc cacgtcaagc tcggccgctt ctggctgcgg cgtgcacggc
     5941 ggctactgcc ggcggtcggg gtgatgatcg cggtgacgat ggtggtcggc gcgatcgtcg
     6001 agcccgaccg ggtcgaccag atgcgcggcg acgcgatcgc ctcgctcgcc tacttcgcca
     6061 actggcactt cgtctacacc gacgtctcct acttcgacca gttccagcgg ccctcgctgt
     6121 tcacccacct ctggtcgctc tcggtcgagg agcagttcta cctcttctgg ccgctcgtct
     6181 tcgccgcggg gatgaagctg ttcgggcgcg gcaagctgct gctcggcgtc gccgccgggg
     6241 cgctcgcctc ggtcgcgctc gcctggatcc tcttcgaccc cggcgccgac gcctcacgga
     6301 tctactacgg caccgacacc cacgcggtcg gcctgctcgc cggcgtcgcg cttgcgttgg
     6361 tctggagccc gatcgagttg cgcaggcgga agaccggtcc gctggtcggc ccgatcctcg
     6421 acgtggtcgg cgtcttcgcg ctcgccttca tcgtgctcag cttcgtcaac gtcaacgact
     6481 acgacctcgg gctctggcac ggcggctacc tctggctggc cctggtcacg acgctcctga
     6541 tcgcggtgat cgtccacccc gccgcccggc tcggcggcct gctcgggcag aagccggtgc
     6601 tctggctcgg gctgcgcagc tacagcttct atctctggca ctggccggtc ctggtcctga
     6661 cccgccccgg cgtcgacgtc gacctgccga ccgaggtcct ggtgccgctg cagctggccc
     6721 tggtcctgct gctctccgac ctctcctacc gctacgtcga gctgcccttc cgcggccagg
     6781 gcgaggtcaa gctctcggcc ggctggctcc gggtggcgcg tccggccctg gtcgccggcg
     6841 tgctcgcggt cgtgctcttc gtcggctgga gcgggctctt ctccagcaac gaggaccgcc
     6901 tcgacgccgc ctccgcggct cagaggcaga ccaaggcggt ggtcgaagcg caacccgaac
     6961 gggtcaagcc cgagggcgag caatccgaac ccggacccga tcacccgccg ggcgcctacg
     7021 agccaccgca gacctaccag gagccgcggg tcccgaccgc gaccaacgcg atgccgcc
//
LOCUS       DASRIG010000222         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1161714, whole genome shotgun sequence.
ACCESSION   DASRIG010000222 DASRIG010000000
VERSION     DASRIG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161714"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFB52_06160"
     CDS             <1..586
                     /locus_tag="VFB52_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZO05952.1"
                     /translation="RSAVESLVLGPLFDGQAVRPDRQTRIIVNAEGSSGVAGPGRNAG
                     HTGRKQAADTYGGAARHGDGALSGKDPSRLDRCAAYAARHAAANVVAAGLATECELML
                     SYAIGEAEPVTVFARTFGRGRLPNERLSAIVREQFDFRPAVIARRFDLWTLPRQRGGR
                     FYRLLAAGGQVGRPDLDLPWERPDMVEALQRAAD"
     gene            complement(702..923)
                     /locus_tag="VFB52_06165"
     CDS             complement(702..923)
                     /locus_tag="VFB52_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05953.1"
                     /translation="MTQSTATLERTNEAAYIPFESPVVLRAADLAPYDEDDAVMDSWL
                     ASLEPREFELILSRLAEADARGPKLEFSA"
     gene            complement(1153..>2145)
                     /locus_tag="VFB52_06170"
     CDS             complement(1153..>2145)
                     /locus_tag="VFB52_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO05954.1"
                     /translation="LSPRWHEYHDQYAKWGYDGSNYPKDGLNGGSRGDSVPPEGGPPG
                     SSLPFLREQLLDEWGIDLAINNPLGHSQKNRHPEYLAALCSATNDWQLAEWTDPEPRI
                     KASITVPSDYAELAAREIDRLGPNPNYVQVLLSIRTAEPLGRPKYWPIYEAAERHGLP
                     VGIHFGGHGGHPITGTGWPSYYVEYHSGMSQSFQSQVISLIYEGVFERFPNLKMVMIE
                     GGFAWLPPLAWRLDAHWKRLRRDLPHLTRLPSEVIHEHIWLTTQPMEEPDRPEHFVEL
                     LEQGNLYDRLMFATDYPHWDFDAPSDALPKIKLPDGFEQKLFFENALGFYRLPS"
BASE COUNT          310 a          742 c          749 g          344 t
ORIGIN      
        1 ccgctcggcc gtcgagagcc tggtgctcgg cccgttgttc gacgggcagg cggtccgccc
       61 ggaccgccag actcggatca tcgtcaacgc cgagggctcg tcgggcgtgg ccggtccggg
      121 ccgcaacgcc ggccacaccg gccgcaagca agccgccgat acctacggcg gcgccgctcg
      181 gcacggcgac ggcgcgcttt ccggcaaaga cccgagccgg ctcgaccgct gcgccgcgta
      241 cgccgcccgc cacgccgccg ccaacgtcgt cgcggcaggc ctcgccaccg agtgcgagct
      301 gatgctctcc tacgccatcg gcgaggccga gccggtcacg gtcttcgcgc ggacgttcgg
      361 gcggggccgc ctgccgaacg aacggctctc ggcgatcgtg cgcgagcagt tcgacttccg
      421 accggccgtg atcgccaggc ggttcgacct ctggaccctg ccgcgccagc gtggcggccg
      481 gttctaccgc ctgcttgcgg ccggcggcca ggtcggccgg ccggacctcg acctgccctg
      541 ggagcgcccc gacatggtcg aggcgctgca gcgcgccgcc gactgacgct tcctgacaat
      601 cccgggcgcg tcggtccgcc gtcgacgggc aatccgctgg gggcaagcaa aaggcccgga
      661 ccgtctggcc cgggccttct ccgctcttgc cgaaggggtt gttacgccga gaactccagc
      721 ttcgggcccc gagcgtcggc ctcggcgagg cggctcagga tcagctcgaa ctcgcgcggc
      781 tcgagcgacg cgagccagct atccatcacc gcgtcgtcct cgtcgtacgg cgcgagatcg
      841 gcggcgcgca gcacgaccgg gctctcgaag gggatgtagg cggcttcgtt ggtccgctcg
      901 agggtcgccg tgctctgcgt catggtcgtt gcacctgtct tcctcgggtc gtgaccgcga
      961 gggcttcgat tactgtctgc accaatcttc gaccaccggc gggggtcggc gcagtacaac
     1021 agcgtacaga tcaggtgtca actccggcac actggacagg ccgccttcgg gtggaagggc
     1081 ctcgtaccag gaatggcgag agccgggccc gacgtcctcg gggctcggct ctcggctagg
     1141 accgcgcgcc ggtcaggacg gcagacggta gaaccccagc gcgttctcga agaacagctt
     1201 ctgctcgaag ccgtccggca gcttgatctt cggcagggcg tcgctcggcg cgtcgaagtc
     1261 ccagtgcggg tagtcggtcg cgaacatcag ccggtcgtac aggttgccct gctccagcag
     1321 ctccacgaag tgctccggcc ggtcgggctc ctccatcggc tgggtcgtga gccagatgtg
     1381 ctcgtggatc acctcggacg ggagccgggt caggtgcggc aggtcgcggc gcagccgctt
     1441 ccagtgtgcg tcgagccgcc aggccagcgg cggcagccag gcgaagccgc cctcgatcat
     1501 caccatcttg aggttcggga atcgctcgaa gacgccctcg tagatcaggc tgatgacctg
     1561 ggactggaag ctctgggaca tcccggagtg gtactcgacg tagtaggacg gccagcccgt
     1621 gccggtgatc gggtgaccgc cgtgcccgcc gaagtggatc ccgaccggca ggccgtggcg
     1681 ctcggccgcc tcgtagatcg gccagtactt cgggcgaccc aacggctcgg cggtccggat
     1741 cgacagcagc acctggacgt agtttggatt cgggccgagg cggtcgatct cacgggccgc
     1801 caactcggcg tagtctgatg ggaccgtgat cgacgccttg atccgcggct cgggatcggt
     1861 ccactccgcg agttgccagt cgttggtggc gctgcagagg gcggcgagat actcgggatg
     1921 gcgattcttc tggctgtgcc cgagcgggtt gttgatcgcc agatcgatgc cccactcgtc
     1981 gagcagctgc tcgcgcagga acggcagact cgatcccggc gggccgccct ccggcggcac
     2041 cgaatcgccc cgagatccgc cgttcaaacc atccttcggg tagttggatc cgtcatagcc
     2101 ccacttggcg tactggtcgt gatactcgtg ccagcgcggc gagag
//
LOCUS       DASRIG010000223         6111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3495611, whole genome shotgun sequence.
ACCESSION   DASRIG010000223 DASRIG010000000
VERSION     DASRIG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495611"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFB52_06175"
     CDS             complement(<1..253)
                     /locus_tag="VFB52_06175"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HZO05955.1"
                     /translation="MLDERWIKSLHVESMRRADLHADREHHVLFQASTIGALLDGAYE
                     GDLSFAELAEHGDLGLGTLNRLDGEMIALDGEFLRADVEG"
     gene            complement(270..1124)
                     /locus_tag="VFB52_06180"
     CDS             complement(270..1124)
                     /locus_tag="VFB52_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05956.1"
                     /translation="MYVPLVRSRLVLTAVAAALVVPGLAACGWGGSGGEGESDDQKVE
                     AAIESALTSTGAAACSESRTAAFMEETTSETGAKAERECEASVQRGEGVPDAVAVSKI
                     KVDGEGASAEVAFKGGEGDGLVLDVALVEEDGAWKIDEFLGIARLDRDRFVNRVIKGV
                     EREGAATTPQLRCLSGALHELSDAQFERAVLGGGNSLTDEIIEECESAGGPEPEPEVL
                     PEYPRAVQQAFLGSCLEQSGSNFAGCECTLEALELEYPVEELLAAEENLASGRAREML
                     EYASLACA"
     gene            1305..3077
                     /locus_tag="VFB52_06185"
     CDS             1305..3077
                     /locus_tag="VFB52_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZO05957.1"
                     /translation="MKRSLSAALLGLAIMLLAPVAAQAAYSNVAPMEVERVRHTATVL
                     PNGKVLVVGGQGVGNQALASAEIFDPATRTWTPTEPMPEPRVNATATLIPGGRVMLVG
                     GTNNGVDSAGVGIYDPATGEWGDGPPLPEARSDHTAIRLSGGRILVAGGSAGTAAQDE
                     AWVLNLKTGNWTATANTLSVPHRWAESALLPDGRVLVYGGSQGGFEATASADIYDPAT
                     NSFTPTGEMAVSRNGAATTTLSDGRILVAGGNGGQWNTPRDSAEIFDPEIEVWLPTAA
                     PLMERTEASAITLQGGKVLLSEWEDSGEIFDPATETWSSAGTTVGARIGMANVLLPDG
                     RVLLTGGCDCEDPFATAELYTPETEAFATPLDFGDQFVGYESPVLHVIIENVGDEALW
                     ADEAELLQTGEAFEVVSNSCGGVRIAPGRTCLLGVHFEPSATGFRSSTLKLEANVDGG
                     FVTTELVAMGVAPPQGEPGDDGGPGTPGTPGLPGTPGTPGADGKAGAQGPKGDAGPQG
                     PQGPAGANAKVTCTSKAKGKGKKVVTTCKVTFAQPLARTARVKLSVGGKTFATGTARK
                     GSRKLTLTASGRHAKGPYVVTVGS"
     gene            3088..3972
                     /locus_tag="VFB52_06190"
     CDS             3088..3972
                     /locus_tag="VFB52_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05958.1"
                     /translation="MSGGFKRSGTFGVVGVIVVAVAVAAIALTMGQGDPNPKLALGLI
                     FGLIAIFMVGLFALQRSDLGRAAGADAAQSSRGAAEAGHAIVDPTVLPEPELWAAMAI
                     APIDTDALRAREEMWGVARGSQNLGIVVTILIFLTVPAIYLTESLVPLLIGGPLIVLT
                     AIYGAFRAIGPGGAIPGSYLLTDRAMEPLGLQVTDKPDGGFEMRFGSPGFDYRLRGWT
                     VLSGRRHGRPVVVRIGGHEDAGTTEVIVGEPSAEWEAKGKRLAVTSGPEGIVVARRKG
                     DGRDWLCDLWLAERLASA"
     gene            4024..4917
                     /locus_tag="VFB52_06195"
     CDS             4024..4917
                     /locus_tag="VFB52_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05959.1"
                     /translation="MGAIELSGVVKRFGPITAVNGLDLDVPEGICLGLLGPNGAGKST
                     TMRLLTGQAIADEGSLRVLNHELPRQSKEARALMGVVPQLDNLDVDVTVEDNLAVYAR
                     LYRVKDVGSAVDKALAIARLQDRRKDEVDKLSGGMRRRLLLARGLVHEPRLVLLDEPT
                     VGLDPQIRTELWTLIGDLKARGTTILMSTHYIEEAERLADEVAVMHQGRIISRGRPAD
                     LIAEHAGRQTAEIYAPPDRLAELRTEAESTGLRVRPAGPAIAIVGSERAENGFAVPDD
                     AVVRAATLEDVFVLLTGEEAE"
     gene            4918..5718
                     /locus_tag="VFB52_06200"
     CDS             4918..5718
                     /locus_tag="VFB52_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05960.1"
                     /translation="MEAQAPPVPRTMRRLEPAAIAGVMSREVANFRTYWKGTTFSSVL
                     EPLVYLLAFGLGLGATLVDEVEGIPYIQFVGTGMVATAVIFSSALPSMFGTFVKHRFQ
                     NTYEAILAAPVDIDELVSAEMLWIAIRSAVYGCVPLIVTFFWGLDPAPGMLLVPIFAF
                     VTALGFAGFGITMAAVVAKIDQFNYVTALFVTPLFLVAGTFFPISGLPEGVQLIANFN
                     PLHQLVELVRGGAFGFEAEDIARFAFLVAFATVTWRVAINRMRARLID"
     gene            complement(5777..6041)
                     /gene="ffs"
                     /locus_tag="VFB52_06205"
     ncRNA           complement(5777..6041)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFB52_06205"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          941 a         2236 c         2028 g          906 t
ORIGIN      
        1 ggccctcgac gtcggcgcgc aggaactccc cgtcgagcgc gatcatctcg ccgtcgagcc
       61 ggttcagcgt gcccagcccg aggtcgccgt gctcggccag ctcggcgaag ctgaggtcgc
      121 cctcgtaggc gccgtcgagc agcgccccga tcgtcgaagc ctggaacagc acgtggtgct
      181 cgcggtcagc gtgcaggtcg gcccgccgca tcgactccac gtgcagcgac ttgatccagc
      241 gctcgtccaa catcaacgcg cagtttcgac tacgcgcagg cgagggacgc gtattcgagc
      301 atttcgcgcg ccctgccgct cgccaagttt tcttcggctg ccagcagttc ctcgaccgga
      361 tactccagtt cgagcgcttc cagggtgcat tcgcagccgg cgaagttcga gccgctctgt
      421 tccaggcacg agccgaggaa ggcctgctgg accgctcgcg gatattcggg cagcacttcg
      481 ggttctggtt ccggaccgcc ggcgctttcg cattcttcga tgatttcgtc ggtcagggag
      541 ttgccgccgc cgagcacggc gcgttcgaac tgcgcatccg acagttcgtg cagcgcaccg
      601 ctgagacagc ggagctgcgg agtggttgcc gcaccttcgc gttcgactcc cttgatgacc
      661 cggttgacga agcggtcccg gtcgaggcgt gcaatgccga ggaactcgtc gatcttccag
      721 gccccgtcct cctcgaccag cgccacgtcg agcacgaggc cgtcgccttc gccgcccttg
      781 aaggcaacct cggcgctcgc cccctcgccg tcgaccttga tcttcgagac cgcgaccgca
      841 tcgggcacgc cttcgccgcg ctgcacgctg gcttcgcact cgcgttcggc cttcgcgccg
      901 gtctcggagg tcgtttcctc catgaaggcc gcggtccgcg actcgctgca ggcagccgcg
      961 ccggtcgagg tcagagcgct ctcgatcgcg gcttcgacct tctggtcgtc gctttcaccc
     1021 tcacccccgc tcccgcccca gccgcaggcc gcgaggcccg gcacgaccaa ggcggccgcg
     1081 acggcagtga ggacgagacg ggatcgcacg agcgggacgt acaccgcggg cgatcctaat
     1141 taattcaggg caccttcgcg cgcgggcact ctcgaccaac agggcggtcc gggctggacc
     1201 gatgatggat ggagttcctt tagtggtcgg cctgcgctgc cgagaactgc tgtgtaggca
     1261 agcgccggcg gggcgcagcc acaacgaact gagggaagaa cccaatgaaa cgctcgctta
     1321 gcgccgccct gctggggctc gcgatcatgc tgcttgcacc ggtcgcggcc caggccgctt
     1381 actccaacgt cgcgccgatg gaagtggagc gcgtccggca cacggccacg gttctgccca
     1441 acggcaaggt gctggtcgtc ggtggccagg gcgtcggcaa ccaggccctc gccagcgccg
     1501 agatcttcga cccggcgacg cggacctgga ccccgaccga accgatgccc gaaccgcgcg
     1561 tgaacgcaac cgcgacgctg atcccgggcg gccgcgtgat gctggtcggc ggcacgaaca
     1621 acggcgtcga ctcggcggga gtggggatct acgaccccgc gacgggggaa tggggcgacg
     1681 gtccgccgct gcccgaagcg cgctcggacc acacggcgat tcgcctctcg ggcggccgga
     1741 tcctcgtcgc cggcggctcc gccggcaccg cggcgcagga cgaggcctgg gtcctcaacc
     1801 tgaaaaccgg caactggacc gcaaccgcga acacgctgag cgtcccgcac cgctgggccg
     1861 agtcggcgct gctcccggac gggcgggtgc tcgtctacgg cggctcccag ggaggcttcg
     1921 aagcgaccgc cagcgccgac atctacgacc cggccaccaa ctccttcacc cccaccggcg
     1981 aaatggcggt ctcccgcaac ggcgccgcga cgacgacgct gtccgacggc aggatcctcg
     2041 tcgccggcgg caatggcggc cagtggaaca cgccgagaga cagcgccgaa atcttcgacc
     2101 ccgaaatcga agtgtggctg ccgaccgcgg caccgctgat ggaacgcacg gaagcatcgg
     2161 cgatcaccct gcagggcggc aaggtgctgc tcagcgaatg ggaagactcc ggtgaaatct
     2221 tcgacccggc caccgagacc tggtcgagcg cgggcaccac ggtcggcgcc cggatcggga
     2281 tggcgaacgt gctcctgccc gacggcaggg tgctgctgac cggcggctgc gactgcgaag
     2341 acccgttcgc gaccgcggaa ctctacacgc cggagacgga agcgttcgcg acccccctcg
     2401 acttcggcga ccagttcgtc ggctacgaga gcccggtcct ccacgtgatc atcgagaacg
     2461 tcggcgacga ggccctctgg gccgacgaag ccgaactgct ccagaccggg gaagccttcg
     2521 aggtggtcag caacagctgc ggcggggtca ggatcgcgcc gggccggacc tgcctactcg
     2581 gcgtccactt cgagccgtcc gcgaccggct tccgctcctc aaccctgaaa ctggaggcaa
     2641 acgtggacgg cggcttcgtg accaccgaac tcgtcgccat gggcgtggcg ccgccgcagg
     2701 gcgaaccggg tgacgacggc ggtcccggaa cgcccggcac ccccggcctc ccgggaactc
     2761 ccggcacgcc tggcgccgac ggcaaagcgg gcgcccaggg cccgaagggc gatgccggcc
     2821 cgcagggtcc ccagggcccg gccggcgcca acgccaaagt cacctgcacc tcaaaagcga
     2881 aaggcaaagg caagaaagtg gtcaccacct gcaaggtgac cttcgcccag ccgctggcgc
     2941 ggaccgcgcg ggtcaagctc agcgtcgggg gcaagacgtt cgccaccggc accgcgcgca
     3001 agggcagccg caagctcacc ctgaccgcct ccgggcgcca cgccaaaggt ccctacgtag
     3061 tcaccgtcgg cagctagtct gccggcggtg tccggcggct tcaaacgatc ggggacgttc
     3121 ggcgtcgtcg gcgtgatcgt cgtcgccgtg gcggtcgcgg cgatcgccct gaccatgggt
     3181 cagggcgatc ccaacccgaa gctggcactg ggcctgatct tcggcctgat cgcgatcttc
     3241 atggtcggcc tcttcgccct gcagcgctcc gacctcggcc gcgccgccgg agccgacgcc
     3301 gcgcagtcga gccgcggcgc cgccgaagcc gggcacgcga tcgtggaccc gacggtgctg
     3361 cccgagccgg agctgtgggc ggcgatggcg atcgccccga tcgacaccga cgcgctccgc
     3421 gcccgcgagg aaatgtgggg ggtcgcccgc ggcagccaga acctcggcat cgtcgtgacg
     3481 atcctgatct tcctcaccgt gccggcgatc tacctgaccg aatcgctcgt ccccctgctg
     3541 atcggcgggc cgttgatcgt gctcaccgcg atctacggcg ccttccgcgc gatcggtccc
     3601 ggtggcgcga tccccggcag ctacctgctg accgaccgcg cgatggagcc gctggggcta
     3661 caggtgaccg acaagcccga cggcggcttc gagatgcggt tcgggagccc cggcttcgac
     3721 taccggctgc gcggctggac cgtgctcagc ggccggcgcc acggccgccc cgtcgtcgtc
     3781 cgcatcggcg gccacgaaga cgccggcacg accgaggtga tcgtcggcga gccgagcgcc
     3841 gagtgggagg cgaagggcaa gcgcctggcc gtgaccagcg gtcccgaagg gatcgtcgtc
     3901 gcccggcgca agggcgacgg gcgcgactgg ctctgcgacc tctggctggc cgagcggctc
     3961 gcctccgctt gagccaacgc tgcgtcaacg agccgggcgc cccaactcgg tagcgtcagc
     4021 tccgtgggag caatcgaact cagtggcgtg gtcaagcgct tcggcccgat cacggccgtc
     4081 aacggactcg acctcgacgt gccggaaggg atctgcctcg gcctgctcgg ccccaacggg
     4141 gccggcaagt cgacgacgat gcggctgctg accgggcagg cgatcgccga cgagggctcg
     4201 ctgcgggtgc tcaaccacga gctgccgcgc cagtcgaagg aagcgcgggc gctgatgggc
     4261 gtcgtaccgc agctcgacaa cctcgacgtc gacgtcacgg tcgaggacaa cctcgcggtc
     4321 tacgcgcgcc tgtaccgggt caaggacgtc ggctccgcgg tcgacaaagc gctcgccatc
     4381 gcccggctcc aggaccgccg caaggacgag gtcgacaaac tctccggcgg gatgcgccgc
     4441 cgcctgctgc tcgcgcgcgg gctcgtccac gagccgcgcc tggtcctgct tgacgagccg
     4501 accgtcggcc tcgacccgca gatccgcacc gagctgtgga ccctgatcgg cgacctcaaa
     4561 gcgcgcggga cgacgatcct gatgtcgacc cactacatcg aggaggccga gcggctcgcc
     4621 gacgaggtcg cggtgatgca ccagggccgg atcatcagcc gcggccgccc cgccgacctg
     4681 atcgccgagc acgccgggcg acagaccgcc gagatctacg ccccaccgga ccggctcgcc
     4741 gagctgcgca ccgaagccga gagcaccggc ctgcgcgtgc ggccggccgg cccggcgatc
     4801 gcgatcgtcg gctccgagcg ggccgagaac gggttcgccg tccccgacga cgcggtcgtg
     4861 cgcgcggcca ccctcgagga cgtcttcgtg ctgctgaccg gcgaggaggc cgagtagatg
     4921 gaggcgcagg cacccccggt cccgcgcacg atgcgccgcc tcgaaccggc tgcgatcgcc
     4981 ggggtgatga gccgcgaggt cgccaacttc cgcacctact ggaagggcac gaccttctcc
     5041 agcgtgctcg aaccgctggt ctacctgctc gccttcgggc tcgggctcgg cgccaccctg
     5101 gtcgacgaag tcgaagggat cccctacatc cagttcgtcg gcaccggcat ggtcgcgacc
     5161 gcggtgatct tctcctccgc cctgccttcg atgttcggaa ccttcgtcaa gcaccgcttc
     5221 cagaacacct acgaagcgat cctcgcggcg ccggtcgaca tcgacgagct ggtcagcgcc
     5281 gagatgctct ggattgcgat tcgctccgcc gtttacggct gcgtgccgct gatcgtcacc
     5341 ttcttctggg gcctcgaccc ggcgcccggc atgctgctgg tcccgatctt cgccttcgtc
     5401 accgcgctcg gcttcgccgg cttcgggatc acgatggcgg cggtggtcgc gaagatcgac
     5461 cagttcaact acgtgaccgc gctgttcgtg acgccgctgt tcctcgtcgc cggcaccttc
     5521 ttcccgatct ccggcctgcc cgagggcgtc cagctcatcg ccaacttcaa ccccctgcac
     5581 cagctggtcg aactggtccg tggcggcgcc ttcggcttcg aggccgagga catcgcgcgc
     5641 ttcgccttcc tcgtcgcctt cgccaccgtc acctggcgcg tcgcgatcaa ccgcatgcgc
     5701 gcccgcctga tcgactgacg ccaacgcaga agttcccgca taccgggaac ttctgcgttc
     5761 gagctgaagt tatggaccgt gcacccgcct tcgagacgag gtcaccgggc gtgttcgtcg
     5821 tttgtggctc gggccgggct tccctgcggc acccgcggca tccgcttaag gctgcttcct
     5881 tccggacctg acctgattcg cgtgcccacg tcgcgcagga cccggccgtc aacgccgccg
     5941 cgacgcactg accccgacgg ccagtccctc ggaacgggaa ttcggccccg ctagagcgga
     6001 ttgcgggtta caaggcaccg ctgattcccc gcctagcacg gtccgttgcc gatcctaccg
     6061 ggccgccata ggatgcgcac ttgctcgcgt tgccgctgcc catcgccgat a
//
LOCUS       DASRIG010000224         8978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2982590, whole genome shotgun sequence.
ACCESSION   DASRIG010000224 DASRIG010000000
VERSION     DASRIG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982590"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /locus_tag="VFB52_06210"
     CDS             complement(<1..2204)
                     /locus_tag="VFB52_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO05961.1"
                     /translation="MNRLAAVLGRRRRWVVGLWILIVLAALPFAAKQTEHLTGGGFDV
                     PGSQSQAVSQSLQDEFGEKVDGISVALQAAPDATAAERAAAVTRVREQVAEVEEVTLT
                     PAAALAGRRQLQETDFALLPLVSQQSSDELIDSAADLRADMEPGSAVDGVTPYLVGQP
                     TIWAGMQEISKEDLQKAETSGFPIVLLILLVVFGSLAAALLPLTLGFVSVLVTGAIIY
                     FISLQMTTSVFVTNMASMIGIGVAIDYSLFILARYREERQTGLSKRESRARALSTSGL
                     AVTFSGIAVIISLAGLWMVDNQALRSMALGAMTVVAVSILTATTLLPALIAMLGDRVM
                     PGGIVGRVAGFFKRRFYRRRTAEQIAASNSGRSEFWEKWTARVMARPWTAVIAVSAVL
                     LALASPLLVMETGTEALAQFPKDSDVRVGNELASEELGGGADPIQVVATFDEPPSAAD
                     KALVAGYARGLSADPGVSSVAPPIYAGDSALVQLTSSAGSESDAAIAVVERLRAEAPA
                     TALAETASVNVGGETARGFDVREQISGSMWKIILFVLALSFVVLMVMLRSLVLPLKAV
                     LMNLLSIGAAYGVLVAIFQWGWFDSLLGFESQGALDTINVPLIFAVVFGLSMDYEVFL
                     MSRIRERYMLHGDNERAVAEGLSSSARTISSAALIMTAVFGVFVLTGVPSIKELGLGC
                     AVAIAIDATLVRLILVPAAMKLMGDWNWWMPVWLDRALPDLSFESGEPEPSKA"
     gene            complement(2301..2930)
                     /locus_tag="VFB52_06215"
     CDS             complement(2301..2930)
                     /locus_tag="VFB52_06215"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO05962.1"
                     /translation="MEAATKAKESAPKERELSGDKAQRIVDAMRNSVAERGAAGSTFE
                     HVAREAGVSRGLLHYYFGTKERLLVEVVRRDAELRITRLDAMLEKAETVDAVLDGLVA
                     SLNDMIENDPGFFLLLYELFSAGRRNPDIRREVGQLFERTRSHVAEVLVAKEAEGVLK
                     LRFDAESVVSYLFALGDGFALQTLSDPERDNSGAMEAGAASARYLLISE"
     gene            complement(2983..3405)
                     /locus_tag="VFB52_06220"
     CDS             complement(2983..3405)
                     /locus_tag="VFB52_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05963.1"
                     /translation="MSKTKAARATDIYESARENPYVQRLIEDEDLRDNLREAFESAKN
                     AYSRATGNGKGPVKAVTSDKKVQKEMRKTAELLQEANERLQKPPRKSRLGKLLLIAVV
                     GAVIALVVSEGARKTVLDALFGAEEEFEYSSTTSINGS"
     gene            complement(3469..5019)
                     /locus_tag="VFB52_06225"
     CDS             complement(3469..5019)
                     /locus_tag="VFB52_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO05964.1"
                     /translation="MEAATLRRGGPAAYLQSARGVLAERSRTFWVVVGLTLLGAVLRF
                     ATLGIQSYHHDEVVTAERVLQANFFDAMDVVRYSESAPPLYYALAWVWTLFTGTGEVG
                     LRSFSALAGVATIPVAYLIGAELRSRRTGLLAAALVAVNPMMLWYSQEGRAYALLTLF
                     SAVSLLFCLRALRNGQRRDFVWWGVFSGLALATHYFAVFPILVEAVLLARRRRRASYA
                     GLAITGGFGLLLLPLAVHQMSYGHAEWIGNMTLGHRLWETAATFVTGETADIIGQPER
                     PELAILPLALCLAAFALLAWRGEREQRRAALVPLLIGAAAIGVPVLLAVLPGGVDFVL
                     ARNMMPALVPLLVVVAIGLTLPGARRLGNAIAAVLLAYSLGFCILASFSPDLQRPDWG
                     AVASRLGEPQGPRATVTWTLAEAPLRYELEAAGSSPAFMVRPSEKLGWMVHEVTFVSG
                     GDVPPPAPKMLGPGFREVDRAQVGGLYMRRYKLQRPGLVPLRLRQLRNARLDYRTNGV
                     LLDGIGPG"
     gene            complement(5035..7419)
                     /gene="lon"
                     /locus_tag="VFB52_06230"
     CDS             complement(5035..7419)
                     /gene="lon"
                     /locus_tag="VFB52_06230"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO05965.1"
                     /translation="MEGTPVLEVVESPLDAEDLIRAGAPLPNALPVLPLRDSVTFPDS
                     LTPLAVGQERSIELVNDVLGANRTLVMVASRDPDNEEPGPDDLYDVGVAGVVARMVKV
                     PDGSLRILVQATQRVRLGEYLTERPYLLAKITELPDVSKESPELQALTRNVQRTFSEI
                     VEQIPYLPEELQLAVANIDDPAALAHLIAGSLRIGVEEKQALLEEVDVTKRLRHLSRI
                     LARELEVVQLGTQIQTQVQSEVDKGQREFFLRQQMKAIQDELGEGDEQQAEATELREK
                     IAAAGLPEHALKAAERELARLEKLPPAAAEHGVIRTYLEWLTELPWSVKTEDDLDIKH
                     ARKVLDADHYDLEKVKDRILEYLAVRKLNPDSPGPILCFVGPPGVGKTSLGKSIAKAL
                     GRKFERISVGGVRDEAEIRGHRRTYIGALPGTIVRALRDSGSKNPVFMIDEIDKMGAD
                     FRGDPASAMLEVLDPAQNDSFRDHYLDLEFDLSDVAFIATANVLDTIPGPLLDRMETI
                     ELAGYTLEEKVHIARQYLVGRQIDANGLKPSQIEFSDAALKAIVEEYTREAGVRNLER
                     QIGTVCRKVAREVAEGKTKRKVKVSAKRARELLGRRRVFAEQRRRTKDPGVATGLAWT
                     PTGGDVLFIEATAMPGSGKLTITGQLGDVMKESAQAALSYVRGHWVEIAPKLGEQWFA
                     EHDIHVHVPAGAVPKDGPSAGVAMTVALSSLVSGRPVRNDVAMTGEVTLTGQVLPIGG
                     LKEKSLAAQRAGIKRVIVPERNQGDVSEIPEHELGSLEFVFVDEVSKALDVALS"
     gene            complement(7419..7862)
                     /locus_tag="VFB52_06235"
     CDS             complement(7419..7862)
                     /locus_tag="VFB52_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZO05966.1"
                     /translation="MPQRDLFADFARMRREMDQMLGDAWGRGRYVSRRTTGFSPNVDV
                     YYCGEPQRAVVKVDLAGVSLSEVGIEVSGRHLAVVGERPVQETEGRVYQQVEIPSGPF
                     RRVVEFQVEVDAQRASATYEDGVLRIELPLRDPEESTHRVPIDRG"
     gene            complement(7880..8065)
                     /locus_tag="VFB52_06240"
     CDS             complement(7880..8065)
                     /locus_tag="VFB52_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05967.1"
                     /translation="MPRKPKAPTCEDCYFHKNMLCALELNETCATFRPNRPEGLVPPR
                     QPVLLMRAPRWATQKAV"
     gene            8204..8842
                     /locus_tag="VFB52_06245"
     CDS             8204..8842
                     /locus_tag="VFB52_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZO05968.1"
                     /translation="MEAYSKRSEKLAASPPLFKSEFLNFFSRVHPAIPALIFVPVIVG
                     MEWLGVNADFGAWALIGLTLAGIAFWTLTEYWLHRLVFHWEPDNAFGRRMHFIIHGIH
                     HDHPNDKLRLVMPPSVSIPLSALFFLAFWLILGDAAFPVFGGFILGYLVYDYTHYYVH
                     HAVPRTKFGKRLREHHMRHHFQDHRYGYGVSSPIWDVAFRTQPRSRKQSDDE"
BASE COUNT         1375 a         3101 c         3090 g         1412 t
ORIGIN      
        1 gctttgctgg gctcgggctc gccgctctcg aagctgaggt ccggaagggc gcggtcgagc
       61 cagaccggca tccaccagtt ccagtcaccc atcagcttca tcgccgccgg caccaggatc
      121 aggcggacca gggtcgcgtc gatcgcgatc gcaaccgcgc agccgaggcc gagctccttg
      181 atcgagggca cgccggtgag cacgaagacg ccgaacaccg cggtcatgat cagcgccgcc
      241 gaggagatcg tgcgagcgct cgaggagagg ccctcggcga ccgcccgttc gttgtcgccg
      301 tggagcatgt agcgctcgcg gatccgcgac atcaggaaca cctcgtagtc catcgagagc
      361 ccgaagacga cggcgaagat cagcggcacg ttgatcgtgt ccagcgcccc ctggctttcg
      421 aagccgagca gactgtcgaa ccagccccac tggaagatcg cgaccaggac gccgtaggcg
      481 gcgccgatcg agagcaggtt catcagcacc gccttcagcg gcaggaccag cgagcgcagc
      541 atcaccatca ggacgacgaa gctgagcgcg aggacgaaga ggatgatttt ccacatcgag
      601 ccactgatct gttcgcgcac gtcgaagccg cgcgcggtct cgcccccgac gttgacgctc
      661 gcggtctcgg ccagcgcggt cgccggcgct tcggcgcgga gtctctcgac cacggcgatc
      721 gccgcgtcgg actcgctgcc ggcgctcgag gtgagctgaa ccagggcgct gtcgccggcg
      781 taaatcggcg gcgccaccga gctgacgccc gggtccgcgg agagtccgcg tgcgtaaccg
      841 gcgaccagcg ctttgtcagc ggcgctgggc ggctcatcga aggtggcgac gacctgaatc
      901 ggatcggcgc cgccgccgag ctcctcggag gcaagttcgt tgccgacccg cacgtcggag
      961 tccttgggga actgggccag cgcttcggtc ccggtctcca tcaccagcag cgggctggcc
     1021 agcgccagca gcaccgccga gacggcgatc accgcggtcc aggggcgcgc cattacccgc
     1081 gccgtccact tctcccagaa ctcgctgcgc ccggagttcg aggcggcgat ctgctcagcg
     1141 gtgcggcggc ggtagaagcg gcgtttgaag aagcccgcga cccggccgac gatccccccc
     1201 ggcatcaccc ggtcgccgag catcgcgatc agtgccggca gcagggtggt ggcggtgagg
     1261 atcgagaccg cgacgacggt catcgccccg agcgccatcg agcgcagcgc ctggttgtcg
     1321 accatccata ggccggccag cgagatgatc acggcgatcc cggagaaggt gaccgcaagg
     1381 cccgaggtgg agagcgcgcg ggcgcgcgac tcgcgcttgc tgaggcctgt ctggcgctcc
     1441 tcccggtagc gggcgaggat gaacagggag tagtcgatcg cgaccccgat cccgatcatc
     1501 gaggccatgt tggtgacgaa gaccgaggtc gtcatctgca gcgagatgaa gtagatgatc
     1561 gcgccggtga ccaggacgct gacgaagccg agggtcagcg gcaggagcgc cgccgccagc
     1621 gagccgaaga cgacgagcag gatcagcagg acgatcggga agccgctggt ctcggctttc
     1681 tgcaggtctt ccttggagat ctcctgcata cccgcccaga tcgtcggctg cccgaccagg
     1741 tagggcgtga cgccatcgac cgcgctgccc ggctccatgt cggcgcgcag gtcggcggcc
     1801 gagtcgatca gctcgtcgga ggactgctgg ctgaccagcg gcagcagcgc gaagtcggtc
     1861 tcctgcagct gccggcgccc ggccagcgcc gcggccggcg tcagcgtcac ttcttcgacc
     1921 tcggctacct gctcgcggac ccgcgttacc gcggcggcac gctcggccgc ggtcgcgtcg
     1981 ggggccgcct gcaacgccac cgagatcccg tcgaccttct cgccgaattc gtcctgcagc
     2041 gactggctga ccgcctgcga ctggctaccg gggacgtcga agccgccgcc ggtcaggtgc
     2101 tcggtctgtt tggcggcgaa cggcagggcc gcgagcacga tcaggatcca gagaccgacg
     2161 acccaccgcc ggcgccggcc gagcactgcc gcaagtcggt tcatcgcgtc tcgcatgagt
     2221 cgagcgcgag cctcccccac cgccaggggg ccggtctgtg agcgccgtca ctgcgtgagc
     2281 agcgtcacgt gtacggggcg ttactcagag atcagcaggt agcgggcgct ggcggcgccg
     2341 gcttccatcg cgccgctgtt gtcgcgctcg gggtccgaga gcgtctgcag ggcgaaccca
     2401 tcgccgagcg cgaacagata agagacaacg gactcggcgt cgaagcgcag cttcagcacg
     2461 ccctcggcct ccttcgcgac cagcacctcg gccacgtgcg agcgggtgcg ctcgaacagc
     2521 tgaccgacct cacgccggat gtcggggttg cgacggccgg cgctgaacag ctcgtagagg
     2581 agcaggaaga acccgggatc gttctcgatc atgtcgttga ggctggcgac cagcccgtcg
     2641 agcacggcgt cgaccgtctc cgccttctcc agcatcgcgt cgagccgggt gatgcgcagc
     2701 tcggcgtcgc ggcgcacgac ctcgactagg agccgctcct tggtcccgaa gtagtagtgc
     2761 aggaggccgc ggctgacccc ggcctcgcgg gcgacgtgct cgaaggtcga gccggcggcg
     2821 ccgcgctcgg cgaccgagtt gcgcatcgcg tcgacgatgc gctgggcctt gtcgccggag
     2881 agttcccgct ctttgggagc ggactctttc gctttcgttg cggcctccat ctatatgcag
     2941 tctaggagag tgggcggccg gcgccgcccc tcccccattc cgctaggacc cgttgatgct
     3001 ggtggtcgag gagtactcga actcctcttc ggcgccaaac agggcgtcga gaaccgtctt
     3061 gcgggcgccc tcgctgacca cgagcgcgat caccgcgccg accacggcga tcagcagcag
     3121 cttgccgagg cggctcttgc gcggcggctt ctgcagccgc tcgttcgcct cctgcagcag
     3181 ctcggccgtc ttccgcattt ccttctggac cttcttgtcc gaagtcacgg ccttgaccgg
     3241 gcccttgccg ttgccggtcg cgcgcgagta ggcgtttttc gccgactcga aggcctcgcg
     3301 gaggttgtcg cgcaggtctt cgtcctcgat caggcgctgc acgtaggggt tttcgcgggc
     3361 actctcataa atgtcggtgg cgcgagccgc cttggtcttg ctcattccct ctcctcgctg
     3421 gattccggaa gcctctcggc agaatagctc caaattgtct ttctacacct accccggacc
     3481 gatcccgtca agcagtacgc cattggtccg gtagtcgagc cgggcgttgc gcagctggcg
     3541 caggcgcagc ggcaccagcc ctggccgctg cagcttgtag cggcgcatgt agagcccacc
     3601 gacctgggcg cggtcgactt cacggaaccc ggggccgagc atcttcggcg ccggcggggg
     3661 cacgtcgcca ccggagacga aggtcacttc gtggaccatc cagcccagct tttcggacgg
     3721 ccggaccatg aacgccgggg acgagcctgc ggcctcgagc tcgtagcgaa gcggcgcctc
     3781 cgccagggtc caggtgaccg tcgcccgcgg cccctgcggt tcgcccagac ggctggcgac
     3841 cgcgccccag tcgggccgct gcaggtccgg cgagaagctc gccaagatgc agaagccgag
     3901 cgagtaggcg agcaggacgg cggcgatcgc gttgccgagc cgccgcgcgc cgggcagcgt
     3961 cagcccgatc gcgaccacga ccagcagcgg caccagggcc ggcatcatgt tgcgggcgag
     4021 gacgaagtcg acgccgccgg ggaggacggc gagcaggacc gggacgccga tcgcggcggc
     4081 gccgatcagc agcggcacca gggcggcgcg ccgctgctcg cgctccccgc gccaggcgag
     4141 cagcgcgaag gcggcaaggc agagcgccag cggcaggatc gccagctccg gccgctcagg
     4201 ctggccgatg atgtcggcgg tctcaccggt gacgaaggtc gccgccgttt cccagagccg
     4261 gtggccgagc gtcatgttgc cgatccattc ggcgtgcccg taggacatct ggtggaccgc
     4321 cagcggcaac agcagcagcc cgaagccgcc ggtgatcgcc aggccggcgt agctggcccg
     4381 ccggcggcgc cgcgccagca gcaccgcctc gaccaggatc gggaagacgg cgaagtagtg
     4441 ggtcgccagc gccaacccgg agaagacacc ccaccagacg aagtcccgcc gctgcccgtt
     4501 gcgcagcgcc cgcaggcaga agagcagaga gaccgcgctg aacagcgtca gcaacgcgta
     4561 ggcgcgcccc tcctgcgagt accagagcat catcgggttg accgcgacca gcgccgccgc
     4621 cagcagcccg gtgcgacggc tgcgcagctc ggccccgatc aggtaggcga ccgggatcgt
     4681 cgccactccg gcgagcgcgg agaacgaacg cagcccgacc tcgccggtgc cggtgaacag
     4741 agtccagacc caggccagcg cgtagtagag cggcggcgcc gactcgctgt agcggaccac
     4801 gtccatcgcg tcgaagaagt tcgcctgcag gacccgttcg gcggtcacca cctcgtcgtg
     4861 gtggtagctc tggatcccca gcgtggcgaa gcgcaggacg gcgccgagca gggtcagccc
     4921 gacgacgacc cagaaggtcc gcgagcgctc cgccaacacc ccccgggcgc tctgcagata
     4981 tgccgccggt cccccccggc gcagggttgc cgcctccact cctaagccat ctccttacga
     5041 gagggcgacg tcgagcgcct tggagacttc gtcgacgaag acgaactcca ggctgccgag
     5101 ctcatgctcg ggaatctcgc tgacgtctcc ctggttgcgc tccggcacga tcacccgctt
     5161 gatcccggcc ctctgcgccg caagcgactt ctccttcagg ccgccgatcg gcagcacctg
     5221 ccccgtaagc gttacctcac cggtcatcgc gacatcgttg cggacgggtc ggccgctgac
     5281 caaagaggac agggcgaccg tcatcgcgac ccctgccgag ggtccgtcct tcggcacggc
     5341 ccctgccggg acgtgcacgt ggatgtcgtg ctccgcgaac cactgctcgc caagtttggg
     5401 ggcgatctcc acccagtggc cgcgcacgta ggagagcgcc gcctgcgccg actccttcat
     5461 cacgtcgccg agctggccgg tgatcgtcag cttgccgctg ccgggcatcg cggtcgcctc
     5521 gatgaagagg acgtcgccgc cggtcggggt ccaggcgagg ccggtggcga cgcccgggtc
     5581 cttggtccgg cgccgttgct cggcgaacac gcggcggcgg ccgagcagct cgcgcgcccg
     5641 tttggccgag accttgacct tgcgtttcgt cttgccctcg gcgacctcgc gggcgacttt
     5701 gcggcagacc gtgccgatct ggcgctcgag gttgcggacg ccggcctcac gcgtgtactc
     5761 ctcgacgatc gctttcagcg ccgcgtcgga gaactcgatc tgggacggct tcagcccgtt
     5821 cgcgtcgatc tggcgcccga caaggtactg gcgcgcgatg tggaccttct cttcgagcgt
     5881 gtagccggcc agttcgatcg tctccatgcg gtcgagcagc ggccccggga tcgtgtcgag
     5941 cacgttcgcg gtggcgatga aggcgacgtc ggagaggtcg aactcgaggt cgaggtagtg
     6001 gtcgcggaag gagtcgttct gggccgggtc gagcacctcg agcatcgccg aggcggggtc
     6061 gccgcggaag tcggcgccca tcttgtcgat ctcgtcgatc atgaagacgg ggttttttga
     6121 gccggagtcg cgcagcgccc gcacgatcgt ccccggcagg gcaccgatgt aggtgcggcg
     6181 gtggccgcgg atctcggcct cgtcgcggac gccgccgacc gagattcgct cgaacttgcg
     6241 ccccagcgcc ttcgcgatcg atttcccgag gctggtcttg ccgactccgg gcggaccgac
     6301 gaagcagagg atcggccccg gcgagtccgg gttgagcttg cgcaccgcga ggtactcgag
     6361 gatccggtcc ttgacctttt cgaggtcgta gtggtcggcg tcgaggactt tgcgcgcgtg
     6421 tttgatgtcg aggtcgtcct cggtcttcac agaccacgga agctcggtca gccactccag
     6481 gtaggtgcgg atgacgccgt gctcggccgc cgccggcggc agtttttcca gccgcgcaag
     6541 ctcgcgctcg gccgctttca gggcgtgctc gggcagcccg gcggcggcga ttttctcgcg
     6601 cagttcggtt gcttcggcct gctgctcgtc gccctccccg agctcgtcct ggatcgcttt
     6661 catctgctgg cgcaggaaga actcgcgctg gcctttgtcg acttcggact ggacctgggt
     6721 ctggatctgg gtgccgagct ggaccacctc gagctcgcgg gcgaggatcc gcgagaggtg
     6781 gcgcaggcgc ttggtcacgt cgacctcctc cagcagcgcc tgcttctcct cgaccccgat
     6841 ccgcagcgag ccggcgatca agtgggcgag cgccgccggg tcgtcgatgt tggcgaccgc
     6901 cagctgcagc tcctcgggca ggtaggggat ctgctcgacg atctccgaga aggtgcgctg
     6961 gacgttgcgg gtcagcgcct gcagctccgg gctctctttg ctgacgtcgg gcagctcggt
     7021 gattttcgcc agcaggtagg gccgctcggt caggtactcg ccgagccgga cccgctgggt
     7081 cgcctggacg aggatccgca gggagccgtc ggggaccttg accatgcgag cgacgactcc
     7141 ggcgacgccg acgtcgtaga ggtcgtcggg cccgggctcc tcgttgtcgg ggtcgcgcga
     7201 ggcgaccatg accagggtgc ggttggcgcc gagcacgtcg ttgacgagct cgatcgagcg
     7261 ttcctggcca accgcgagcg gggtcagcga gtcagggaag gtgaccgagt cgcgcagcgg
     7321 cagcaccggc agggcattgg gcagcggcgc tccggcgcgg atcagatcct ccgcgtcgag
     7381 cggcgattcg accacctcga ggacaggcgt gccctccatc aaccgcggtc gatcggcacg
     7441 cggtgggtgc tctcttcggg gtcgcggagc ggcagctcga tccgcagcac cccgtcctcg
     7501 taggtggcgc tggcgcgctg ggcgtcgacc tcgacctgga actcgaccac gcgccggaag
     7561 gggccggacg ggatctcgac ctgctggtag acgcggccct cggtctcctg cacggggcgt
     7621 tcgccgacca ccgcgaggtg gcggccggag acctcgatcc cgacctcgct cagcgagaca
     7681 ccggcgaggt cgaccttgac cacggcccgc tgcggctcgc cgcagtagta gacgtcgacg
     7741 ttgggcgaga agccggtggt gcggcggctg acgtagcggc cgcgacccca ggcgtcgccc
     7801 agcatctggt ccatctcacg gcgcatgcga gcgaagtcgg cgaatagatc gcgctggggc
     7861 atcgcccaga aaaatagctc tagacggctt tctgggttgc ccagcgcggc gcccgcatca
     7921 gcagcaccgg ctggcggggc ggcaccaggc cctcgggccg gttgggccgg aaggtcgcgc
     7981 aggtctcgtt cagctcgagc gcgcaaagca tgttcttgtg gaagtagcag tcctcgcagg
     8041 tgggggcttt gggcttgcgc ggcatcgact cgggcatcgt gccactcagg cgtgcctggc
     8101 gtgaggggct accccgcaaa tcgccaactc tttgcaacag cttcgtgaca tcttccccgc
     8161 gtcttgcgaa cgcacgggtt cgcggcgcta agatgccgcc cggatggagg cttactcaaa
     8221 gcgctccgag aagctcgcgg cctcgccgcc gcttttcaag agcgaatttc tcaacttctt
     8281 ctcccgcgtc cacccggcga tcccggcgct gatcttcgtg cccgtgatcg tgggcatgga
     8341 gtggctgggg gtaaacgccg acttcggcgc ctgggcgctg atcgggctga cgctggccgg
     8401 gatcgcgttc tggacgctga ccgagtactg gctgcaccgc ctggtcttcc actgggagcc
     8461 cgacaacgcc ttcggccgcc gcatgcactt catcatccac ggcatccacc acgaccatcc
     8521 caacgacaag ctgcgtctgg tgatgccgcc ctcggtctcg atcccgctgt ccgcgctctt
     8581 cttcctcgcc ttctggctga tcctcggcga cgcggccttc ccggtcttcg gcggcttcat
     8641 cctcggctac ctggtctacg actacacgca ctactacgtg caccacgccg tcccgaggac
     8701 gaagttcggc aagcggctgc gcgagcacca catgcgccac cacttccagg accaccgcta
     8761 cggctacggc gtctcctcgc cgatctggga cgtcgccttc cgcacgcagc cgcgcagccg
     8821 caagcagagc gacgacgagt agctaagccg cgcgcagcgc cagctccaac tcgaaccgcg
     8881 cctcggggtc gtcgagctgc tcgccaagca gctctcggag ccgggcgatc cggtagcggg
     8941 cggtctgggg gtggatgtgg agctcgtttg ccatcgcg
//
LOCUS       DASRIG010000225         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_362817, whole genome shotgun sequence.
ACCESSION   DASRIG010000225 DASRIG010000000
VERSION     DASRIG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362817"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFB52_06250"
     CDS             <1..307
                     /locus_tag="VFB52_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05969.1"
                     /translation="GPALEASLEMTTRGGRIVVAGIPKKPVPVDAGRMLLYERSLLTA
                     LGYHNDLPRVAAMIANGSLKPDSMITRRVPLAETPAEIARLAGDPGDDVKVLVEVGG"
     gene            337..534
                     /locus_tag="VFB52_06255"
                     /pseudo
     CDS             337..534
                     /locus_tag="VFB52_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
     gene            1120..1815
                     /locus_tag="VFB52_06260"
     CDS             1120..1815
                     /locus_tag="VFB52_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZO05970.1"
                     /translation="MIPTPRVRKVIAEHMVRSRQTAAHMTTEVDVDLSATVDARAELN
                     AARLAAGEPKLSYLPFIARAACAALAEYPELNATFLGEEMVRWQQVNLGVAVDTPEGL
                     LVPVIKGCGQLTAPQIGAAIADLAERARTRKLTPDDLAGGTFTLSNPGSVGAVSAPAI
                     INQPQVAILGVPTIQRRPWVVTGPDGTETVAIRPILRLAVTFDHRALDGADATRFAVA
                     VKNRLEAWTTESE"
     gene            1826..2629
                     /locus_tag="VFB52_06265"
     CDS             1826..2629
                     /locus_tag="VFB52_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05971.1"
                     /translation="MTHPRVLVLQSFRTEAVPRWIERCIESVRDWATANGWEHRLLGD
                     ELFDTVPEWFASKLGGNLVPMSDLARIETMLRCFATEPWDRIVWVDADVLVFAPERLR
                     IPDLRRPQLVRELWIGHLPDGYVVEEKVNNSVIACVRGDSELTALRDETIEAIRQSQK
                     PDQLDAGTRMLTARQAVRPLPLLDAVGCFGPLVIRDLEAGGGEALDLLLEACEVPPAA
                     ANLCRSLGENEGELGMVTEAGFERSVDVLLGTGNPIAAAFERGGPSLAV"
     gene            complement(2626..>2886)
                     /locus_tag="VFB52_06270"
     CDS             complement(2626..>2886)
                     /locus_tag="VFB52_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO05972.1"
                     /translation="PLDMETIVASVEKTGRALVVHEAVRTAGPGAEIAARIQESAFFA
                     LDAPVWRLGALDTPVPQSPDLEQLVIPSGEQIVAAARELVAI"
BASE COUNT          415 a         1026 c         1041 g          404 t
ORIGIN      
        1 tggcccggcg ctggaagcct cgctggagat gaccacgcgc ggtggccgca tcgtcgtcgc
       61 cgggatcccg aagaagccgg tcccggttga cgccggccgc atgctgctct acgagcgctc
      121 gctgctgact gcgctcgggt accacaacga cctgccgcgg gttgcggcga tgatcgccaa
      181 cggctcgctc aagccggact cgatgatcac ccgccgcgtc ccgctcgccg agaccccggc
      241 cgagatcgcg cgcctcgccg gggaccccgg cgacgacgtc aaggtcctgg tcgaggtcgg
      301 cgggtgagcg agacggcgac gactgtcgtt gtgccgatgc cccacatggg ggtttcggtc
      361 gaggagggca cggtggtcga gtggctggtc tccgtcgggg acgaggtgac ggcggaccaa
      421 cccatctgcg caatcgcgac cgacaaggtc gacaccgagg tcgtcgcgcc ggccgatggc
      481 gtgctggctc ggatcgtggc ggccgtcggt gatgacgttc cggtcggcgg agcgctcgct
      541 gagatggcgg ttggggacga cgcggcggca gtccttgagg cggtgggggt cgaggggggt
      601 gactcggcgg acgcgactcg gggtggagag gttgctgctg acgcctcagg cgtggccggc
      661 ccctcgcgca gagtgtccgg cgaaccaccc ccctcgaccc tctccagctc aggggctgac
      721 cgcgtcgaca cggacgccgg cttggcggag cgggcggcgg cggggcgcga tgagtttgga
      781 cggttcgatc cgcggaaagc ggcggaagcg gtgctcagct tcgtggccgc tccagcaggc
      841 gcgcaagtgg aggcgaaggt gctcgcgtct cccgtggcca agcggatggc agcgagccac
      901 aacgtcgatc tcgcgagcgt gcgagggacg ggccgcgccg ggagagttcg aaagcgcgac
      961 gtggagcaag cgatcgccgg gggcggtcaa tttgttcggg atggccgaac aaatcgccaa
     1021 cctccgtcca ccccctcccc tacccagcct gtttcgacgg cggccggcgc ccgagcgagc
     1081 gccgaattgc cccccggcta cgccgatatc ccgcacgagg tgatccccac cccccgtgta
     1141 cgcaaggtga tcgccgagca catggtgcgc tcgcgccaaa ccgcggccca catgacgacg
     1201 gaggtcgatg tcgatctcag cgccaccgtc gacgctcgcg ccgagctgaa cgccgcgcgc
     1261 ctcgccgccg gcgaaccgaa gctctcctac ctgccgttca tcgctcgcgc cgcttgcgct
     1321 gcgctggcgg agtaccccga actgaacgcg accttcctcg gcgaggagat ggtgcgctgg
     1381 cagcaggtca acctcggcgt cgccgtcgac acgcccgagg gcctgctcgt cccggtgatc
     1441 aaaggctgcg gccagctcac ggccccccag atcggcgccg cgatcgccga cctcgccgag
     1501 cgcgcccgca cccgcaagct cacccccgac gacctcgccg gcggcacctt caccctctcc
     1561 aacccaggct cggtcggcgc cgtctccgct ccggcgatca tcaaccagcc ccaggtcgcg
     1621 atcctcggcg tgcccacgat ccagcgccgc ccctgggtcg tcaccggccc cgacggcacc
     1681 gagaccgtcg cgatccgccc gatcctccgc ctcgccgtca ccttcgacca ccgcgccctc
     1741 gacggcgccg acgccacccg cttcgcggtc gcggtcaaga accgcctcga agcctggacg
     1801 acggagtccg aataactttc cgccggtgac gcacccgcgt gtactggtcc tgcagtcctt
     1861 ccgcaccgag gcggttccgc gctggatcga gcgctgcatc gagagcgtgc gcgattgggc
     1921 cacggccaac ggatgggagc accggctgct cggcgacgag ctgttcgaca ccgtcccgga
     1981 gtggttcgcg agcaagctcg gcggcaacct cgtgccgatg tcggacctgg cgcggatcga
     2041 gacgatgctc cgctgcttcg cgaccgagcc ctgggaccgg atcgtctggg tcgacgccga
     2101 cgtgctcgtg ttcgcgccgg agcgcctgcg catccccgat ctgcgccggc cgcagctggt
     2161 ccgggagctg tggatcggcc acctgcccga cggctacgtg gtcgaagaga aggtcaacaa
     2221 cagcgtgatc gcgtgtgtgc gcggcgactc cgagctgacc gcgctcaggg acgagacgat
     2281 cgaggcgatc cggcagtccc agaagcccga ccagctcgac gccggcacca ggatgctgac
     2341 cgcgcgccag gccgtccggc cactgccgct gctcgacgcg gtcggctgct tcggcccgct
     2401 cgtgatcagg gacctggagg cgggcggcgg cgaggcgctc gacctgctgc tggaggcctg
     2461 tgaggtgccg cccgcggcgg ccaacctctg ccgctcgctg ggcgagaacg agggcgagct
     2521 ggggatggtc accgaggcgg gcttcgaacg cagcgtcgac gtcctgctcg ggaccggcaa
     2581 cccgatcgcc gccgcgttcg agcgcggcgg cccctcgctc gcggtctaga tcgcgaccag
     2641 ctcgcgggcc gcggcgacga tctgctcgcc gctgggaatg accagctgct cgaggtcggg
     2701 gctctgcggc accggggtgt cgagggcgcc gaggcgccag acgggggcgt cgagggcgaa
     2761 gaaggcggac tcctggatgc gggcggcgat ctcggcgccg gggccggcgg tgcggacggc
     2821 ctcgtggacg acgagggcgc ggccggtctt ctcgaccgag gcgacgatcg tttccatgtc
     2881 gagggg
//
LOCUS       DASRIG010000226         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2983400, whole genome shotgun sequence.
ACCESSION   DASRIG010000226 DASRIG010000000
VERSION     DASRIG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983400"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFB52_06275"
     CDS             complement(<1..504)
                     /locus_tag="VFB52_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO05973.1"
                     /translation="MTSLGLKSLWARKVRALTTTFAVVLGVAFMAGSYVLTDTIFAAF
                     DQIFGESVKGTSVVVSSKNLVKQQGGETPTIEESLLAPIKRTPGVRLASGAIFTQGGF
                     FDDKGDEVGAKLDFKAISSVLPGSLESLDYEQGHPPRGPTEVSLGESAAREAGLGIGD
                     TLEIVGRI"
     gene            complement(497..1228)
                     /locus_tag="VFB52_06280"
     CDS             complement(497..1228)
                     /locus_tag="VFB52_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO05974.1"
                     /translation="MSVNGKVVVATDLTRRYGEGDTAVDALAGVSTAFDRGRFTAIMG
                     PSGSGKSTLMHILAGLDKPTNGTVELDGVEITALDDGELTKLRRDKLGFVFQFFNLLP
                     VLTAEENMTLPLSIAGRKPDPEWLERLVETVGLGDRRTHLPAELSGGQQQRVAVARAL
                     LSKPAVVFADEPTGNLDSKSSGDVLALLRQAVDEFDQTVVMVTHDPGAAAHADRLITL
                     RDGKVVHDAAPGSADDVIELMKTVD"
     gene            1268..2050
                     /locus_tag="VFB52_06285"
     CDS             1268..2050
                     /locus_tag="VFB52_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZO05975.1"
                     /translation="MPIADQFDGLLIDLDGVVWIGREPIPGSAEALAALLAAGKQLVF
                     VTNNPTKAPASYAKRLQGMGVDVGPERIVTAGMVTARLAGEAADGGGAFVIGAEPLKE
                     MVAAEGAELLTGEPAYAAKVVVVSGHRGFDFEELLTAKRALDAGAALVATSHDPTMPM
                     PGGEWPGTGSILAAVETASGRRAEIGGKPEHHLFELARKQLGGAERVAMVGDRVSSDI
                     AGGKAAGLATILVLSGTTSESEAANADPAPDHVVADLAALLR"
     gene            2047..>3086
                     /locus_tag="VFB52_06290"
     CDS             2047..>3086
                     /locus_tag="VFB52_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO05976.1"
                     /translation="MTPAEADAAPYAGRSFAALFAVTFSGLVAIGAVLPVLPRYVHGP
                     LGYGDVAVGIVIGAYAITGLLLRPFAGRLADRRGRKPAVLLGIVGVSLGGLLYLPSLG
                     LAGVILARLVTGAGEGALYTAGSAWIVDMAPEARRGRVLGLYGLAVWGGLSVGPLAGE
                     LLLDLGGYNAVWIFAAALPLLGALIASRVPDPFVPHTHAEPHPLIAPEAIGPGIAVAL
                     AAVGYAAIASFVVLHLEARGIDDGVVVFGAFAAMIVITRLVAGGLPDRVGPVRVAIVA
                     GFAEAGGLLTIALAQDLTVAIIGGAAMGAAFSLINPSLMLIALGRVTPAARGAAMGTY
                     TAFFDAGVGLGAP"
BASE COUNT          401 a         1101 c         1108 g          476 t
ORIGIN      
        1 aatgcggccg acgatctcca gcgtgtcgcc gatcccgagc cccgcttccc tggcggcgct
       61 ttcacccagc gatacttcgg tcggcccgcg tgggggatgt ccctgctcgt agtcgaggga
      121 ctcgaggctg ccgggcagca ccgaggagat cgccttgaag tcgagtttgg cgccgacttc
      181 gtcgcccttg tcgtcgaaga atcccccctg cgtgaagatc gcgcccgagg ccagtcgcac
      241 ccccggcgtc cgcttgatcg gggccagcag agattcttcg atcgtcggcg tttctccccc
      301 ctgctgtttg accaggtttt tcgagctgac gacgacgctc gtgcccttca ccgattcgcc
      361 gaagatctgg tcaaaggcgg cgaagatcgt gtcggtgagg acgtaggagc cggccatgaa
      421 ggcgaccccg agcacgaccg cgaaggtggt ggtgagcgcg cgaaccttgc gagcccagag
      481 gctcttgagg ccgaggctag tcaaccgtct tcatcagctc gatcacgtcg tcggccgagc
      541 cgggcgcggc gtcgtggacg accttgccgt cgcgcagcgt gatcaggcgg tcggcgtggg
      601 cggcggcccc agggtcatgg gtaaccatga ccacggtttg atcgaactcg tcgactgcct
      661 ggcgcagcag ggcgagcacg tcgccgctgg atttcgagtc gaggttgccg gtcggctcgt
      721 cggcgaagac cacggccggc ttggagagca gcgcccgggc gaccgcaacc cgttgctgct
      781 gaccgcccga gagctccgcc ggcaggtggg tgcggcggtc gccgaggccg acggtctcga
      841 ccagccgctc gagccactcg gggtcgggct tgcgacccgc gatcgagagc ggcagggtca
      901 tgttctcctc ggcggtcagc accggcagca ggttgaagaa ctgaaagacg aagccgagct
      961 tgtcacggcg cagcttggtc agctcaccgt cgtcgagtgc ggtgatctcg accccgtcga
     1021 gctcgacggt gccgttggtg ggcttgtcga ggccggcgag gatgtgcatc agggtcgact
     1081 tgcccgagcc cgaggggccc atgatcgcgg tgaaacgacc acgatcgaaa gcggtgctga
     1141 ccccggcaag ggcgtcgacg gcggtgtcgc cctcgccgta gcggcgggtc aggtcggtgg
     1201 ccacgacgac ctttccgttg acgctcacga cgacaccgta tcgagacgag tgaatagcct
     1261 ctctccggtg ccgatcgccg accagttcga tggacttctg atcgacctcg acggggtcgt
     1321 ctggatcggc cgtgagccga tccccggctc ggccgaggcg ctggcagcgc tgctggcggc
     1381 cggcaaacag ctcgtcttcg tcaccaacaa cccgaccaag gcgccggctt cctacgccaa
     1441 gcgcctgcag ggaatgggcg tcgacgtcgg ccccgagcgg atcgtcaccg cgggcatggt
     1501 gaccgcgcgc ctcgccggcg aagcggcgga cggcggcggc gccttcgtga tcggggccga
     1561 gccgctgaag gagatggtcg cggcggaagg agccgagctg ctgaccgggg agccggccta
     1621 cgcagccaag gtcgtcgtcg tctccggaca ccgcggcttc gacttcgagg agctcttgac
     1681 cgcgaagcgg gcgctcgacg ccggagcggc gctggtcgcg accagccacg acccgacgat
     1741 gccgatgccg ggcggcgagt ggccgggcac cggctcgatc ctggccgcgg tcgaaaccgc
     1801 gagtgggcgg cgcgccgaga tcggcggcaa gcccgagcac cacctgttcg agctggcgcg
     1861 caagcagctc ggcggcgccg agcgggtggc gatggtcggc gaccgcgtct cctccgacat
     1921 cgccggcggc aaggccgccg ggctggcgac gatcctggtg ctcagcggca ccaccagcga
     1981 gtccgaagcc gccaacgcgg acccggcgcc cgaccacgtg gtcgccgacc tcgctgcgct
     2041 gctgcggtga ccccggccga ggcggacgcc gccccctacg ccggccgcag cttcgcggcg
     2101 ctcttcgccg tcaccttctc cggcctggtc gcaatcggcg cggtgctccc ggtcctgccc
     2161 cgctacgtcc acggcccgct cggctacggc gacgtcgcgg tcgggatcgt gatcggcgcc
     2221 tacgcgatca ccggcctgct gctgcggccg ttcgccggcc ggctcgccga ccgccgcgga
     2281 cgcaagccgg ccgtcctgct gggcatcgtc ggggtctcgc tcggcgggct gctctacctg
     2341 ccgagcctcg gcctcgccgg cgtgatcctc gcccgcttgg tgaccggcgc cggcgagggc
     2401 gcgctctaca ccgccggctc ggcctggatc gtcgacatgg cgccggaggc gcggcgcggg
     2461 cgcgtgctgg gcctctacgg gctcgctgtc tggggcgggc tcagcgtcgg cccgctggcg
     2521 ggcgagctgc tgctcgacct cggcggctac aacgccgtct ggatcttcgc cgcggcgctg
     2581 ccgctgctcg gcgcgctgat cgcctcgcgg gtccccgacc cgttcgtgcc ccacacccac
     2641 gccgagccgc acccgctgat cgcgccggag gcgatcgggc ctggcatcgc cgttgccctc
     2701 gccgcggtcg gctacgcggc gatcgcctcg ttcgtcgtcc tccacctgga agcgcgcggg
     2761 atcgacgacg gggtggtcgt gttcggcgcc ttcgcggcga tgatcgtgat cacccggctg
     2821 gtcgccggcg gtctccccga tcgggtcggc ccggtgcgcg tggcgatcgt cgccggcttc
     2881 gccgaggccg gaggcctgct gacgatcgcc ctggcccagg acctcacggt cgcgatcatc
     2941 ggcggcgcgg cgatgggcgc cgccttctcg ctgatcaacc cctcgctgat gctgatcgcg
     3001 ctcggccgcg tcaccccggc ggcgcgcggc gcggcgatgg gcacctacac cgccttcttc
     3061 gacgccggcg tcggcctcgg agcccc
//
LOCUS       DASRIG010000227         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_885103, whole genome shotgun sequence.
ACCESSION   DASRIG010000227 DASRIG010000000
VERSION     DASRIG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885103"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFB52_06295"
     CDS             <1..1007
                     /locus_tag="VFB52_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZO05977.1"
                     /translation="DHLKHELTILACAFADSEGGIDAAYERAAETIEAMRERLRGAVP
                     APEAPSVTEPPEFVSNMTREEFEAAVARIVEYVHAGDAFQVVPSQRFSAPAPVEAFSV
                     YRGLRAVNPSPYMYFLEFGDFQIAGASPEPLVKVNGNRVETRPIAGTSPRGKSVEEDR
                     RLAERLIADPKERAEHVMLVDLGRNDLGRVCEYGTVKVDEMMEIETYSHVLHIVSQVS
                     GTLREGVSSLDVLRSALPAGTLSGAPKVRAMQIIDELEKVKRCGYGGAIGYLSWDGDL
                     DTAIHIRTVVCKDGQLHVQAGGGTVADAKPNYEWHESVNKAKAVFRAVEVACEQPDWA
                     "
     gene            1013..1585
                     /locus_tag="VFB52_06300"
     CDS             1013..1585
                     /locus_tag="VFB52_06300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZO05978.1"
                     /translation="MAKTTVLVIDNYDSFTYNLVQYLGELGAELEVVRNDKATVAELL
                     ERGADRLVVSPGPCAPAEAGISGEAIRAFAEAGTPVLGVCLGHQAMVEVFGGKVVQGE
                     PVHGKEAEVEHDGKSIYAGLPTPMVAGRYHSLIADPDLPDELELTASLDGIVMGARHR
                     ELPAEGVQFHPESVLTPQGKSLLANFLGEG"
     gene            1586..2614
                     /gene="trpD"
                     /locus_tag="VFB52_06305"
     CDS             1586..2614
                     /gene="trpD"
                     /locus_tag="VFB52_06305"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZO05979.1"
                     /translation="MPNDVVSRAIDAVCAGDHLTADHASAVLAEIMEGRTDPVQTGAF
                     LIALRAKGETVPELVGLARTMRGLATPVSSRRPDLVDTAGTGGGPTTFNVSTTAALVA
                     AGAGCAVAKHGNRSATTKSGSADLLEALGVNIDLEPEQVGRCIDEVGFGFMFAPKHHS
                     AMAHVIPARKALGVRTIFNFLGPLTNPAGANRQLIGIADRHYQETIAEALVSLGSVRA
                     LVVAAEDGLDEFSLAARTRVIEVADGGTSEWFVEPGQFGLAPADLDSVAGGDPADNAA
                     VTRAVLAGEPGPKRDLVLLNAAAAIYVGGLVPDLEAGVAKAAEAVDSGAAADVLERLV
                     EATRPGGV"
     gene            2634..3434
                     /gene="trpC"
                     /locus_tag="VFB52_06310"
     CDS             2634..3434
                     /gene="trpC"
                     /locus_tag="VFB52_06310"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZO05980.1"
                     /translation="MIEQLIDGARRGVDARRQAVPQAELESRLSARTEDRPFNEALVR
                     PGLSLIAEFKRRSPSRGDISADADVASQVAAYERGGAAALSVLTDEPHFGGSLEDLRA
                     ARGACRLPIIRKDFIVDPYQLYEAAVHGADAVLLIVRALDDDRLRELYEEARAIDLDC
                     LVEVHDAEELERALEADAEVIGINNRDLDEQRVDIATTFELMPDVPAGKTVVAESGIS
                     GREELEELDRVGVDAVLIGGALMDAADPEAKARELTGLDEGTREHRLP"
     gene            3533..>4608
                     /locus_tag="VFB52_06315"
     CDS             3533..>4608
                     /locus_tag="VFB52_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZO05981.1"
                     /translation="MNETNAPQSPRKSSFRSSFAAALIGGALVAAFGWIAIASGLIKA
                     DSDSTTTVAAPMTAPVSSSGSDDSEGNTVNQIYKADGDGVAFISAEMAAQEPETFSPF
                     GTPESEGGGTATGSGFVIDDEGHVLTNNHVVEGASKITVKLGDSETAHEAEVVGTDPA
                     TDVALLKVDAPADELHPLTLGDSSQMEVGDPVVAIGNPYGLDRTVTSGIVSALQREIQ
                     APNGFSITNVIQTDAAINPGNSGGPLINGAGEVIGINSQIETGGGSSGNVGIGFAVPI
                     DTVKAELQQLKETGEVEHAFLGISGATITPELAQAINLPVKEGVLVQEAVKDGPADKA
                     GIEGGDTTGTIEGVEVSLGGDIIT"
BASE COUNT          746 a         1659 c         1569 g          634 t
ORIGIN      
        1 tcgaccacct caagcacgag ctgacgatcc tcgcctgcgc cttcgccgac tccgagggcg
       61 ggatcgacgc cgcctacgag cgcgccgccg agacaatcga ggcgatgcgg gagcggttgc
      121 gcggcgccgt gccggcgccg gaggcgccca gcgtcaccga gccgcccgag tttgtctcca
      181 acatgacccg ggaggagttc gaagcggcgg tcgcgcggat cgtcgagtac gtgcacgccg
      241 gcgacgcctt ccaggttgtc ccctcgcagc gcttcagcgc gcccgccccg gtcgaagcgt
      301 tctcggtcta ccgcggcctg cgcgcggtca acccctcgcc ctacatgtac ttcctcgagt
      361 tcggcgattt ccagatcgcc ggcgcttcgc ccgagccgct ggtcaaggtc aacggcaacc
      421 gggtggagac gcggccgatc gccggcacct ccccccgggg caagagcgtg gaggaggacc
      481 ggcgcctcgc cgagcggttg atcgccgacc ccaaggagcg ggccgagcac gtgatgctgg
      541 tcgacctcgg ccgcaacgac ctcggccggg tctgcgagta cggcacggtc aaggtcgacg
      601 agatgatgga gatcgagacc tactcgcacg tcctccacat cgtcagccag gtctccggca
      661 ccctgcgcga gggggtcagc tctctcgacg tgctgcgttc ggcgctcccg gccggcaccc
      721 tctccggggc gcccaaggtg cgcgcgatgc agatcatcga cgagctcgag aaggtgaagc
      781 gctgcggcta cggcggcgcg atcggctacc tctcctggga cggcgacctc gacaccgcga
      841 tccacatccg caccgtcgtc tgcaaggacg ggcagctgca cgtacaggcc ggcggcggca
      901 cggtcgccga cgcgaagccc aactacgagt ggcacgagtc ggtgaacaag gcaaaggcgg
      961 tcttccgcgc cgtcgaggtc gcctgcgaac agccggactg ggcgtgagcg cgatggcgaa
     1021 gaccacggtc ctggtgatcg acaactacga ctccttcacc tacaacctcg tccagtacct
     1081 gggcgagctg ggggcggagc tggaggtcgt gcgcaacgac aaggcgacgg tcgccgagct
     1141 gctcgagcgc ggcgccgacc ggctggtcgt ctcgcctggc ccctgcgcgc ccgccgaggc
     1201 ggggatctcc ggtgaggcga tccgcgcctt cgccgaggct ggcacgccgg tgctcggcgt
     1261 ctgcctgggc caccaggcga tggtcgaggt gttcggcggc aaggtggtcc agggcgagcc
     1321 ggtgcacggc aaggaggccg aagtcgagca cgacggcaag tcgatctacg ccggcttgcc
     1381 gacaccgatg gtcgccggcc gctaccactc gctgatcgcc gaccccgatc tgcccgacga
     1441 gctcgagctg accgccagcc tcgacgggat cgtgatgggt gcccgccacc gggagctacc
     1501 cgccgagggc gtccagttcc accccgagag cgtgctcacg ccgcagggga aatcgctgct
     1561 cgcaaacttc ctcggggagg ggtagatgcc aaacgacgtc gtctcgcgcg cgatcgacgc
     1621 ggtctgcgcc ggtgaccacc tgaccgccga ccacgcctcg gcggtgctcg ccgagatcat
     1681 ggaaggccgc accgatccag tccagaccgg cgctttcctg atcgccctgc gggcaaaggg
     1741 cgagacggtg ccggagctgg tcggcctggc gcggacgatg cgcggcctcg cgacgccggt
     1801 ctccagccgc cgacccgacc tggtcgacac ggccgggacc ggcggcggtc cgacgacctt
     1861 caacgtctcg accacggcgg cgctggtcgc cgccggcgcc ggttgcgcgg tcgccaagca
     1921 cggcaaccgc tcggccacga cgaaaagcgg ttccgccgac ctgctggaag cgctcggcgt
     1981 caacatcgac ctcgagcctg agcaggtcgg tcgctgcatc gacgaggtcg gcttcgggtt
     2041 catgttcgcg cccaagcacc actcggcgat ggcgcacgtg atcccggccc gcaaagcgct
     2101 cggcgtgcgc acgatcttca acttcctcgg gccgctgacc aacccggccg gcgcgaaccg
     2161 gcagctgatc gggatcgccg accgccacta ccaggagacg atcgccgagg ctctggtcag
     2221 cctcggcagc gtgcgggccc tcgttgtcgc cgccgaggac gggctcgacg agttctcgct
     2281 cgcggcccgc acgcgggtga tcgaggttgc cgacggtggc acctccgagt ggtttgtcga
     2341 gcccggccag ttcggcctcg cgccggccga cctcgactcg gttgccggcg gcgatcccgc
     2401 cgacaacgcc gccgtgaccc gggccgtgct ggccggcgaa ccggggccca agcgcgacct
     2461 cgtcctgctc aacgcggccg ccgcgatcta cgtcggcggc ctcgtccccg acctcgaggc
     2521 cggggtcgcg aaggcggccg aggcggtcga ctcgggcgcg gcagcggacg tgctggagcg
     2581 actggtcgag gcaacccgcc ccggcggcgt ctaggctccc gtaggcagtg ggaatgatcg
     2641 agcagctgat cgacggcgcc cggaggggtg tcgacgcacg tcgccaggcg gtgccgcagg
     2701 ccgagctgga atcgcgcctg tccgcgcgca ccgaagaccg gcccttcaac gaggccctgg
     2761 ttcggcccgg cctttcgctg atcgccgagt tcaagcgccg ctcgccgagc cgcggcgaca
     2821 tctccgccga cgccgacgtc gcctcccagg tcgccgccta cgagcgcggt ggcgccgctg
     2881 cgctctcggt gctgaccgac gaaccgcatt tcggcggctc gctcgaggac ctgcgcgcgg
     2941 cgcgcggcgc ctgccggctg ccgatcatcc gcaaggactt catcgtcgat ccctaccagc
     3001 tctacgaggc cgcggtccac ggtgccgacg cggtgctgct gatcgtccgc gcgctcgacg
     3061 acgaccggct gcgggagctg tatgaggagg cgcgggcgat cgacctcgac tgcctggtcg
     3121 aggttcacga cgccgaggag ctggagcgcg cgctcgaggc cgacgccgag gtgatcggga
     3181 tcaacaaccg cgacctcgac gagcagcggg tggacatcgc caccaccttc gagctgatgc
     3241 ccgacgtgcc ggccggcaag accgtggtcg ccgagagcgg catctccggt cgcgaggagc
     3301 tggaagagct cgaccgggtc ggcgtcgacg cggtcctgat cggcggcgcc ctgatggacg
     3361 ccgccgatcc cgaggcgaag gcgcgcgagc tgaccggcct cgacgagggc acccgcgagc
     3421 accgcctccc ttagacgcgc cgatcggcgc gcgcgtaggg agcttcggct ctttagaaaa
     3481 cgttcattga ggatttaagg acgtctcagc gcgggcggcc attctggtcg tcatgaacga
     3541 gaccaacgcg ccccaatccc cccgtaagtc ttccttccga tcctcctttg ccgccgccct
     3601 gatcggcggc gcgctggttg ccgcgttcgg ctggatcgcg atcgcctccg gtctgatcaa
     3661 ggccgacagc gactcgacca cgaccgtagc cgcaccgatg accgccccgg tctcgtcctc
     3721 gggctccgac gactcggagg gcaacaccgt caaccagatc tacaaagccg acggcgatgg
     3781 cgtcgccttc atctcggccg aaatggccgc gcaggaaccc gaaaccttct cgcccttcgg
     3841 cacccccgaa tccgaaggcg gtgggaccgc caccggctcc ggcttcgtga tcgacgacga
     3901 aggccatgtg ctgaccaaca accacgtggt cgaaggcgcg agcaagatca cggtcaaact
     3961 cggcgactcc gagaccgcgc acgaggccga agtcgtcggc accgacccgg ccaccgacgt
     4021 tgccctgctc aaggtcgacg cgccggccga tgaactgcac ccgctgaccc tcggcgactc
     4081 ctcgcagatg gaagtcggcg acccggtggt cgcgatcggc aacccctacg ggctcgaccg
     4141 caccgtcacc agcggcatcg tctccgccct gcagcgcgag atccaggccc cgaacgggtt
     4201 ctcgatcacc aacgtgatcc agaccgacgc ggcgatcaat cccggcaact ccggcggtcc
     4261 gctgatcaac ggcgccggcg aggtgatcgg gatcaactcc cagatcgaga ccggcggtgg
     4321 cagcagcggc aacgtcggca tcggcttcgc cgtcccgatc gacaccgtca aggccgagct
     4381 ccagcagctc aaggagaccg gcgaagtcga acacgctttc ctcggcatca gcggcgccac
     4441 gatcacgccc gaactggccc aggcgatcaa cctgccggtc aaagaagggg tcctggttca
     4501 ggaagccgtc aaagacgggc ccgccgacaa ggccgggatc gagggcggcg acacgaccgg
     4561 gacgatcgaa ggggtcgaag tcagccttgg cggcgacatc atcaccga
//
LOCUS       DASRIG010000228         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_363079, whole genome shotgun sequence.
ACCESSION   DASRIG010000228 DASRIG010000000
VERSION     DASRIG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363079"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFB52_06320"
     CDS             <1..722
                     /locus_tag="VFB52_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05982.1"
                     /translation="TAFAGAAETPKAGTSAAKVSATLQKLKTQLASLRQQVKGLEGQS
                     GTPRTPTGPAGGDLAGAYPNPLVGPNTIGAQELLPNSVGNSELQDNAVGSDELQNESV
                     GGEKVKLNTLTGFNIIDATIGAGDIANNAIFSSQIADGSVGAADLGVNSVGSSELAPL
                     IATLSAGKAINAGTPQNVSVTCPGNRTLIAGGYAWTQDEGNSIIASAPSEADPTHTWV
                     VRGMVDEGSNTLFAWATCITG"
     gene            954..4541
                     /locus_tag="VFB52_06325"
     CDS             954..4541
                     /locus_tag="VFB52_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO05983.1"
                     /translation="MSFPVPPFARRFLAGAALVLSLLLLSSASASAAVLDLENLDGPT
                     AVEMMENGELTSVELTQAYIDRINALNQTGPGLNAVSQLNKAALAEAQKTDELRAEGK
                     ILSPAMGLPILLKDLIDVQGMYTSAGNWSLRDSYPETDSGVATKLKEHGVVILGKLGL
                     SEYANYFGRQASGFGNLTGQVLNGLDTDQTPSGSSSGSGTAGAAALSMLTIGTETSGS
                     ITSPSRTQGLVGIRPTIGLVPGYGIAPIVASQDTAGPMDRTVANAALTLTSIAGSNSV
                     NDAYYEDYWGELELEPEDIAPPVPNPLPDYLSALDPQFVKGKRIGYTGSNPEVLAAKQ
                     ALEDAGAILVERPAISTPAMPTIAGDWYIEMHMAIDHYYERLGAAAPINSLAEEVADN
                     EANAHEALKYGNFRHKESSEWEYGPGTANSKEVSEKLPVRKEIAQNGIELMLENGTPG
                     TPADDFIAIIGSNNSSPIANGPLAGYPVITVPMGYSATQRRAIGVSIGGGAYSERDLI
                     GVAYVLEQQTMERKPASEVNPSMYRCADTVPAPAFAERGDCNPDHDTALAAAGGAVPD
                     IDFSLETTSATELQEMMEAGELTAAELTKAYLARIAVSNAAGPAIQAVRNINANAVAE
                     AEALDAEREKSGPRGPLHGIPVLLNDGIDAKGLASEGGSIALQDAMPEDNSRIVSKLK
                     GAGAIVLGTANVTELGAMVSDDMTDGYSSLGGQVLMPSDTDDAPAGSSAGAAAAVSSG
                     MAAMAVGVETSQASAELIGPAGVNGVVGLKPTVGRVSRAGVMPVAKSQDSPGPIAQTV
                     TDVAAQLQVIAGKDVNDAATTSAPAVPDYLAGLDEGALSGLGIATITHSTSNPETVKP
                     VYDAAIATVTGKGATTTNVATPNPTLPDITTDEFGRDLDAYLAGLSGGADSLDEIVAY
                     NKANPVEGLKFQQGDLLEAAAVDLTDPTQKTAYEGELASSKEGAKTALDAILASNDLI
                     MVPSESSLVDLADKAGYPVLSVFGGYGLESMGRNPVGVAFIAAPFEEAELLAAGYAFE
                     AASSERLAPSWTNPSMYRCVELSEFYSPHHCRPGLVEPLVVVPPTDGPPSPPPAAGTP
                     KLTVKAVPKSRIVGAARKTVTFRLVARNGGDAATGTVSLCAAAPKARVTVLGKKCTTV
                     EIPAGASKTRSVQLKIKPKAVGKSTKVTLTASGITVGKQSVKATLTVKD"
BASE COUNT          861 a         1742 c         1501 g          599 t
ORIGIN      
        1 cgacggcgtt tgccggcgcc gccgagaccc ccaaggccgg cacctcggcc gccaaggtca
       61 gcgccaccct gcagaagctg aaaacccagc tggcctccct gcgccagcag gtcaagggcc
      121 tcgaaggcca gagcggcacc ccgcgcaccc ccaccggccc cgccggcggt gacctcgccg
      181 gtgcttaccc gaaccccctg gtcggcccga acacgatcgg cgcccaggag ctgctgccga
      241 actcggtcgg caactccgag ctgcaggaca acgcggtcgg cagcgacgag ctgcagaacg
      301 aatcggtggg cggcgaaaag gtgaagctca acaccctcac cgggttcaac atcatcgatg
      361 ccacgatcgg cgccggcgac atcgccaaca acgccatctt cagcagccag atcgccgacg
      421 gctccgtcgg cgcggctgac ctgggagtga acagcgtcgg ctcctccgag ctggcgccgc
      481 tgatcgccac cctctcggcc gggaaagcga tcaacgccgg cacgccgcag aacgtctcgg
      541 tcacctgccc cggcaaccgc accctgatcg ccggcggcta cgcctggacc caggacgagg
      601 gcaactcgat catcgcctcc gccccgagcg aagccgaccc gacccacacc tgggtcgtgc
      661 gcggcatggt cgatgagggc agcaacaccc tgttcgcctg ggccacctgc atcaccggct
      721 aggcaccggg cggtcggccc ccaacccgaa tcgacccggc aataagactc cacatagggg
      781 ttcgattgcc gggtcgattc ttgatgtaca ggcagtccag gtggctgtgc cgaactcgct
      841 acccgcggtc cattgttcgg gggccaaccc gtaaatagct tgggagcggt ctgggaaccc
      901 ggcatcccta ccgcccagac ggccccccac ccccgagcac tggagtccgt cacttgagct
      961 tccccgtccc cccgttcgcc cggcgctttc tggccggcgc ggccctcgtc ctctccctcc
     1021 tccttctcag cagtgcttcc gcctcggcgg ccgtgctcga cctcgagaac ctcgacggcc
     1081 cgaccgccgt ggagatgatg gaaaacggcg aactgacctc ggtcgaactg acccaggcct
     1141 acatcgaccg gatcaacgcg ctgaatcaga cggggccggg gctgaatgcg gtctcgcagc
     1201 tcaacaaggc agcgcttgcg gaagcgcaga aaaccgacga actgcgggcg gagggcaaaa
     1261 tcctcagccc ggcgatgggg ctgccgatcc tgctcaagga tctgatcgac gtgcagggga
     1321 tgtacacgtc ggcgggcaac tggtcgctgc gcgactccta ccctgaaacc gactccggcg
     1381 tcgccacgaa gctgaaagaa cacggcgtcg tgatcctcgg caagctcggc ctctccgagt
     1441 acgccaacta cttcggccgc caggcctccg gcttcggcaa cctgaccggc caggtcctca
     1501 acggcctcga caccgaccaa actccgagcg gctcctcctc gggctccggc accgccggcg
     1561 ccgcggcgct ctcgatgctg acgatcggaa ccgagacctc gggctcgatc acctcgccct
     1621 cgcgcaccca gggcctggtc gggatccggc cgacgatcgg cctcgtcccc ggatacggga
     1681 tcgccccgat cgtcgcctcg caggacaccg ccggcccgat ggaccgcacg gtcgccaacg
     1741 cggccctgac cctgacctcg atcgccggct cgaactcggt caacgacgcc tactacgagg
     1801 actactgggg cgagctcgaa ctcgagcccg aagacatcgc gccgccggtg ccgaacccgc
     1861 tgccggacta cctctcggcg ctcgacccgc agttcgtcaa aggcaagcgg atcggctaca
     1921 ccggctccaa ccccgaagtc ctcgccgcca aacaggcgct tgaagacgcc ggcgcgatcc
     1981 tggtcgaacg accggcgatc agcaccccgg cgatgccgac gatcgccggt gactggtaca
     2041 tcgagatgca catggcgatc gatcactact acgagcggct cggcgccgct gcgccgatca
     2101 actcgctcgc cgaggaggtc gccgacaacg aagcgaacgc ccacgaggcg ctcaagtacg
     2161 gcaacttccg ccacaaagaa tcgtccgaat gggaatacgg cccgggcacg gccaactcca
     2221 aagaagtttc cgaaaaactc ccggtccgca aggaaattgc ccagaacggg atcgagctga
     2281 tgctcgaaaa cgggaccccg ggcaccccgg ccgacgactt catcgcgatc atcggctcca
     2341 acaactcgag cccgatcgcc aacggcccgc tcgccggcta cccggtgatc acggtgccga
     2401 tgggctacag cgccacgcag cgccgggcga tcggcgtctc gatcggcggt ggcgcctaca
     2461 gcgagcgcga cctgatcggc gtcgcctacg tgctcgaaca gcaaacgatg gagcgcaagc
     2521 cggccagcga ggtcaacccg agcatgtacc gctgcgccga cacggtcccg gcgccggcct
     2581 tcgccgagcg cggcgactgc aaccccgacc acgacaccgc cctcgcagcg gccggcggcg
     2641 ccgttcccga catcgacttc tcgctggaga cgacgtcggc cacggaactg caggagatga
     2701 tggaagcggg cgaactgacc gctgccgagc tgaccaaggc ctacctggcc cggatcgcgg
     2761 tctccaacgc cgccggtccg gcgatccagg cggtccgcaa catcaacgcc aacgcggtcg
     2821 ccgaggcgga agcgctcgac gccgagcgcg agaaatccgg cccacgcggt ccgctgcacg
     2881 gcatcccggt cctgctcaac gacgggatcg acgccaaagg cctggccagc gagggcggct
     2941 cgatcgccct ccaggacgcg atgcccgagg acaactcgcg gatcgtctcc aagctcaagg
     3001 gcgccggggc gatcgtcctc ggcaccgcca acgtcaccga gctgggggcg atggtcagcg
     3061 acgacatgac cgacggctac agctcgctcg gcggtcaggt gctgatgccc tccgacaccg
     3121 acgacgcgcc cgccggctcc tcggccggtg ccgccgccgc ggtctcctcc gggatggcgg
     3181 cgatggcggt cggcgtggag acctcgcagg cctcggccga gctgatcggg cccgccggcg
     3241 tcaacggcgt cgtcggcctg aagccgaccg tcggccgggt cagccgcgcc ggcgtgatgc
     3301 cggtcgccaa atcccaggac tcgcccggcc cgatcgccca gacggtcacc gatgtggcgg
     3361 cccagctgca ggtgatcgcc ggcaaagacg tcaacgacgc ggccacgacc agcgccccgg
     3421 cggtgccgga ctacctggcc gggctcgacg agggggcgct cagcgggctg ggcatcgcca
     3481 cgatcaccca ctccaccagc aacccggaaa cggtcaaacc ggtctatgac gcggcgatcg
     3541 ccacggtgac cgggaaaggc gcgaccacca cgaatgtggc gacgccgaac ccgacgctcc
     3601 ccgacatcac caccgacgag tttgggcgtg acctcgacgc ctacctcgcc gggctctccg
     3661 gcggtgccga cagcctcgac gagatcgtcg cctacaacaa agcgaatccg gtcgaggggc
     3721 tgaagttcca gcagggcgac ctgctggaag cggccgcggt cgatctcacc gatccgaccc
     3781 agaaaaccgc ctacgaaggt gagctcgcat ccagcaagga aggcgcgaaa acggcgctcg
     3841 acgcgatcct ggccagcaac gacctgatca tggtcccgtc cgaaagcagc ctggtcgacc
     3901 tggccgacaa ggccgggtac ccggtgctga gcgtgttcgg cggctacggg ctggagtcga
     3961 tgggtcgcaa cccggtcggc gtcgccttca tcgccgcgcc gttcgaagag gcggagctgc
     4021 tcgccgccgg ctacgcgttc gaagcggcca gcagcgagcg cctcgcgccc agctggacca
     4081 acccgagcat gtaccgctgc gtcgagctca gcgagttcta ctcgccgcac cactgccgcc
     4141 cgggcctggt cgaaccgctg gtggttgtgc cgccgaccga cgggccgccg agcccgccgc
     4201 cggcagccgg cacgccgaag ctgaccgtga aagcggtgcc gaagtcccgg atcgtcggcg
     4261 ccgctcgcaa gacggtcacg ttccggctgg tcgcccgcaa cggcggcgac gccgccaccg
     4321 gcacggtcag cctctgcgcc gccgcgccga aagcgcgggt cacggtgctg ggcaagaagt
     4381 gcaccacggt cgagatcccg gccggggcga gcaagacgcg ctcggtccag ctgaagatca
     4441 agccgaaggc agtcggcaag tcgaccaagg tcaccctgac cgccagcggg atcaccgttg
     4501 gcaagcagag cgtcaaggcg acgctcacgg tcaaggactg aggaggaaga ggcgccccgg
     4561 acaggggccg gggcgcctcc tctcagccgg ttcgaccgac ggtccaggcg cctcgaccga
     4621 actcgctact cgcggtccat tgttcggggg ccggaccgta aatagcttgg gagcggtctg
     4681 ggaacccggc atccctaccg ccc
//
LOCUS       DASRIG010000229         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3498504, whole genome shotgun sequence.
ACCESSION   DASRIG010000229 DASRIG010000000
VERSION     DASRIG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3498504"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFB52_06330"
     CDS             <1..735
                     /locus_tag="VFB52_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO05984.1"
                     /translation="WVAIFNDVSEERRTRDKAERLEAAKAEFLGSVSHELRTPLTSIL
                     GYAALLQDDADEQTRKHLDVIERNASRQLRLVEDLLSIARIQAGEFEVVRIPVDFAEI
                     VELGAEAARPDAERAGLGLKVDVLGPVSLQGDADRLGQVVANLVSNAIKFTPAGGRVE
                     VTLRSDGRRARLIVADTGPGVRSSERTQLFDRLFRGEDARETEVSGAGLGLSIARSIV
                     EAHEGRIDLAEGTGSGATFEVALPLG"
     gene            745..1620
                     /locus_tag="VFB52_06335"
     CDS             745..1620
                     /locus_tag="VFB52_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO05985.1"
                     /translation="MPEAQSPLSVVIPAREGIVEIAPVLAGLEEEAREAGVEVIVVGP
                     ASGGGAPEWVRLIEMDDPSMLALRRRAVLAARGEIVAIGEDHAVPRPGWCRDVIRAHA
                     EHPEAAVVIGCLVNATDDTVAGRANFLAFAAAWQPPMPELPSRRPGPSSTMTFKRSAY
                     EGLEEQPVGWFEATLIPALFLAGRMVADDRIVVDHYQDHGAVWSVINAYDSARSSYGS
                     DRPRLSPAERRRVARWAAAEIPKGLWREARAGAGTKGMSAPVAALVATIVSAAGLGAV
                     LGCLVGPGRSAERVA"
     gene            1679..3451
                     /locus_tag="VFB52_06340"
     CDS             1679..3451
                     /locus_tag="VFB52_06340"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZO05986.1"
                     /translation="METLAPKARRARSIVFAAVLATLALLGLALGAGRAEASQVGPVG
                     LEFCIPIGGCTLATSARVDQRPAVIPSFFVTNPIGSGGSLFVELPAGATFNGVPEVQL
                     NYGVGGFSVQGESLANENRRLQVPVPAFLIQKEGSLNSSKEVELLIGVGAEAVILPAT
                     PGNFSVQVWTTSDVEARTSSSLATTLGLPATIAPVAGRSATVGTEFAEPPAVYLKDSR
                     GNPIPDAELGFEVPNGPGPSGNFEGPQVDSAGETNSEGVATPNLPLTANTVAGDWLLK
                     VEGPNDTEGSVPMTNLAGEADEIELEIDPTVLPADGGTEAGATITVTDQFGNHVTTDE
                     IALDSPVDGPAIGAPVLQEDGTFTADLLSSTVPGEYAVTATDSSVDPELTDSVTVTQS
                     VLPATGISLALSPPSILADGSSTTTALIEVENEIGGPVAKEDIAVASSSGNSIGSVVD
                     RGDGTYTAVITSTAAVGSVTITATDASTDPDLKATASLSQTALPPPPPPPPPPATTPS
                     VKITAGPKGKTKAKRATFKFAVTSGSAAYFECKLDKAKWTRCKSPKGFAVKPGPHTFS
                     VRGVGADGSAGPVAKRSFKRLPPK"
     gene            3489..>4859
                     /locus_tag="VFB52_06345"
     CDS             3489..>4859
                     /locus_tag="VFB52_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZO05987.1"
                     /translation="MSTLAPQEPSTQGLTQAEAERRLREHGPPEKEATSRSYVSIAVA
                     NTFTVFNGILAAFGAATIAFGNPKDALFLGILVANTFIGTFQEVRAKRALDKLAALVA
                     PAATVIRDGEPREVPVGEVVVGDLVRLRSGDQVVADGNLLRAEGLAIDESNLTGESEA
                     VVRNPGDEVLSGSFAVEGEGDFEATAVGADSRAAKLAATAKAFRHPRSPLEKAMDRLL
                     IILVGVMLPLGIALGISLAIRDVSQEDAVETLTAAIVNIVPEGLILLVSLTAAVSAAK
                     MARRGILAQQLNAIESLASVSVMCTDKTGTLTEASLRVVSLVPATGVGEDELARSLAR
                     YAASSETRNATLEAVHAAQLGGGETAAEEASAIVPFSSRRRWSGLELGGERLVFGAPE
                     RLLADAASETALRERAAADAGDGRRVLALASADGALPEATPTAELPGGLRPLGIVVLA
                     EELRANA"
BASE COUNT          715 a         1790 c         1736 g          618 t
ORIGIN      
        1 tgggtggcga tcttcaacga cgtcagcgag gagcgccgca cgcgcgacaa ggctgagcgg
       61 ttggaggcgg cgaaggccga gttcctcggc tcggtctcgc acgagctgcg cacgccgctg
      121 acctcgatcc tcggctacgc cgcgctgctc caggacgacg ccgacgagca gacacgcaag
      181 cacctcgacg tgatcgagcg caacgccagc cgccagctgc ggctggtcga ggacctgctc
      241 agcatcgccc gcatccaggc gggcgagttc gaggtcgtgc gcatccccgt cgacttcgcc
      301 gagatcgtcg agctcggggc cgaggcggcg cgcccggacg ccgagcgggc cgggctgggg
      361 ctgaaggtcg acgtgctcgg cccggtgtcg ctgcagggcg acgccgaccg gctcggccag
      421 gtcgtcgcca acctcgtctc caacgcgatc aagttcaccc ccgccggggg gcgggtcgaa
      481 gtgaccctgc gcagcgacgg ccggcgcgcc cggctgatcg tcgccgacac cgggcccggg
      541 gtgcgctcga gcgagcgcac ccagctcttc gaccgcctct tccgcggcga ggacgcgcgc
      601 gagaccgagg tctccggggc cggcctcggc ctctcgatcg cgcgctcgat cgtcgaggcg
      661 cacgaaggcc ggatcgacct cgccgagggc accgggtcgg gcgcgacctt cgaagtcgcg
      721 ctgccgctgg gctagattgc cgcggtgccc gaggcccagt cgccgctctc cgtcgtgatc
      781 cccgcccgcg agggcatagt cgagatcgcc ccggtcctgg ccggcctcga agaagaagcg
      841 cgcgaggccg gggtcgaagt gatcgtcgtc ggcccggcca gcggcggcgg cgcgcccgag
      901 tgggtgcggc tgatcgagat ggacgacccg agcatgctgg cgctgcggcg ccgggcggtg
      961 ctggcggcgc gcggcgagat cgtcgcgatc ggcgaggacc acgcggtgcc gcggccgggc
     1021 tggtgccggg acgtgatccg cgcccacgcc gagcaccccg aggccgcggt cgtgatcggc
     1081 tgcctggtca acgcgaccga cgacacggtc gccgggcgcg ccaacttcct cgccttcgcc
     1141 gccgcctggc agccgccgat gccggagctg ccgagccgcc gccccggccc ctcctcgacg
     1201 atgaccttca agcgctccgc ctacgagggg ctggaggagc agccggtggg ctggttcgag
     1261 gcgaccctga tcccggcgtt gttccttgcc ggccggatgg tcgccgacga ccgcatcgtc
     1321 gtcgaccact accaggacca cggcgcggtc tggtcggtga tcaacgccta cgactcggcc
     1381 cgctcctcat atggctccga ccgtccccgc ctctcccccg ccgaacgccg gcgggtcgcc
     1441 cgctgggcgg ccgccgagat tcccaaagga ctgtggcggg aggcgcgggc gggcgccggg
     1501 acgaagggga tgagcgcgcc ggtggcggcg ctagtggcaa cgatcgtctc ggccgccggg
     1561 ctcggcgcgg tgctcggctg cctggtcgga cctggacgct cggccgagcg cgtcgcctga
     1621 cgctcgtcca ggcgtggccg gcgggtccgg acacgctggt actttgcggc gctcaatggt
     1681 ggaaacgctc gcaccaaagg cgcgccgcgc gcgctcgatt gtgttcgccg cggtcctcgc
     1741 gacgctcgcc ctgctcggac tggcgctagg cgccggccgc gcggaggcat cgcaggtcgg
     1801 gccggtgggc ctcgaattct gcatcccgat cggaggctgc acgctcgcga cgagcgcccg
     1861 ggttgaccag cggcccgcgg tgatcccctc cttcttcgtc accaacccga tcggctccgg
     1921 cggctcgctg ttcgtcgaac tgccggccgg ggcgaccttc aacggcgtcc cggaagtgca
     1981 gctcaactac ggggtcggcg gcttctcggt ccagggggag agcctggcga acgaaaaccg
     2041 gcgcctgcag gtcccggtgc cggccttcct gatccagaaa gaagggtcgc tcaactcctc
     2101 gaaagaggtc gaactgctga tcggcgtcgg cgccgaagcg gtgatcctgc cggcgacgcc
     2161 gggcaacttc agcgtccagg tctggaccac ctccgacgtc gaagcgcgga cttcctcgtc
     2221 gctcgcgacc acgctcggcc tgccggccac gatcgcgccg gtcgccggca ggtcggcgac
     2281 ggtcgggacc gagttcgccg agccgcccgc cgtctacctc aaagactcgc gcggcaaccc
     2341 gatcccggac gccgagctgg gcttcgaagt gcccaacggc cccggtccga gtgggaattt
     2401 cgaagggccg caggtggact cggccggcga aaccaactcc gagggggtcg cgacgccgaa
     2461 tctgccgctg accgcgaaca ccgtcgccgg cgactggctg ctgaaggtcg agggaccgaa
     2521 cgacaccgaa ggctcggtcc cgatgaccaa cctcgccggc gaggccgacg agatcgagct
     2581 tgaaatcgac ccgaccgtgc tgccggccga cggggggacc gaagccggcg cgacgatcac
     2641 cgtgaccgac cagttcggca accacgtcac cactgacgag atcgccctcg acagcccggt
     2701 cgacgggccg gcgatcggcg cgccggtgct gcaggaagac ggcaccttca cggccgacct
     2761 gctctcctca accgtgcccg gcgaatacgc ggtcaccgcc accgacagct cggtcgaccc
     2821 ggagctcacc gactcggtca ccgtcaccca atcggtgctg ccggcaaccg ggatctcgct
     2881 cgcgctctcg ccgccgagca tcctcgccga cggcagctcg accacgaccg cgctgatcga
     2941 ggtcgagaac gagatcggcg gcccggtcgc caaagaggac atcgcggtgg ccagctcgag
     3001 cggcaactcg atcggctcgg tcgtcgaccg cggcgacggc acctacaccg ccgtgatcac
     3061 cagcacggcc gcagtcggct cggtgacgat cacggcgacc gacgccagca ccgatcccga
     3121 cctaaaagcg acggcgagcc tgtcccagac ggcgctgccg ccccctcccc cgccgccgcc
     3181 accaccggcc accaccccga gcgtgaaaat caccgccggg ccgaaaggca aaacgaaagc
     3241 caagcgcgcg accttcaagt tcgccgtcac cagcggcagc gccgcctact tcgagtgcaa
     3301 gctcgacaaa gccaaatgga cgcgctgcaa gtcgccgaag ggcttcgcgg tgaaacccgg
     3361 cccgcacacc ttcagcgtcc gcggcgtcgg ggccgacggc agcgccggcc cggtcgccaa
     3421 gcgctccttc aagcgcctgc cgccgaagta gtccgggccg ccgggcggct cgaatacgct
     3481 ccccgcggat gagcacgctc gccccccagg agccctcgac ccaggggctg acccaggccg
     3541 aggccgagcg gcgcctgcgc gagcacgggc cgccggagaa ggaggcgacc agccgctcct
     3601 acgtctcgat cgcggtcgcc aacacgttca cggtcttcaa cgggattctg gctgccttcg
     3661 gcgccgcgac gatcgccttc ggcaacccca aggacgcgct cttcctcggc atcctcgtcg
     3721 ccaacacctt catcggcacc ttccaggagg tacgcgccaa gcgggcactc gacaagctgg
     3781 cggcgctggt cgcgccggcg gcgacggtga tccgcgacgg cgagccgcgc gaggtgccgg
     3841 tcggcgaggt cgtggtcggc gacctggtgc ggctgcgcag cggcgaccag gtggtcgccg
     3901 acggcaacct gctgcgcgcc gaggggctgg cgatcgacga gtcgaacctg accggcgagt
     3961 cggaggcggt cgtccgcaac cccggcgacg aagtcctctc cggatcgttc gcggtcgagg
     4021 gcgagggcga cttcgaggcg accgcggtcg gcgccgacag ccgcgcggcg aaactggcgg
     4081 ccacggcgaa ggccttccgc cacccgcgct cgccgctgga gaaagcgatg gaccggctgc
     4141 tgatcatcct cgtcggggtg atgctgccgc tcgggatcgc cctggggatc tcgctggcga
     4201 tccgcgacgt cagccaggaa gacgcggtcg agacgctcac cgcggcgatc gtcaacatcg
     4261 tccccgaggg gctgatcctg ctggtctcac tgaccgcggc ggtctcggcg gcgaaaatgg
     4321 cgcggcgcgg gatcctcgcc cagcagctca acgcgatcga gtcgctggcc tcggtctcgg
     4381 tgatgtgcac ggacaagacc gggacgctga ccgaggcctc gctgcgggtc gtctcgctgg
     4441 tccccgcaac gggggtcggc gaggacgagc tggcgcgcag cctcgcccgc tacgcggcca
     4501 gctcggagac gcgcaacgcg accctggagg cggtgcacgc ggcgcagctc ggcggtggcg
     4561 agaccgcggc cgaggaggcg agcgcgatcg tccccttctc ctcgcggcgg cgctggagcg
     4621 gcctggagct gggcggcgaa cggctcgtct tcggcgcgcc ggagcggctg ctcgcggacg
     4681 ccgcgagcga gaccgcgctg cgcgagcgcg ccgccgccga cgccggcgac gggcgccgcg
     4741 tactggcgct cgccagcgcc gacggagccc ttcccgaggc cacgccgacg gccgagctgc
     4801 cgggcgggct gcggccactc gggatcgtcg tgctcgccga ggagctgcgc gccaacgcg
//
LOCUS       DASRIG010000230         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_640263, whole genome shotgun sequence.
ACCESSION   DASRIG010000230 DASRIG010000000
VERSION     DASRIG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640263"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFB52_06350"
     CDS             complement(<1..402)
                     /locus_tag="VFB52_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZO05988.1"
                     /translation="MGERLTAGFYDRSVHEVARDLIGCRLFFDGVGGTIVETESYERD
                     DPACHAYVGLTERTRVLFGPPGYAYVYLSYGIHSLLNAVAEPEGEAAAVLIRALEPTH
                     GVEEMRTRRGGKADADLCSGPGKLTEALGIDL"
     gene            complement(413..1813)
                     /gene="argH"
                     /locus_tag="VFB52_06355"
     CDS             complement(413..1813)
                     /gene="argH"
                     /locus_tag="VFB52_06355"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZO05989.1"
                     /translation="MSRQRLRKEPDPRFWRLNRSLEFDKWLAPYDLDQSRAHARGLQR
                     IGVLDADELAQMEAGLDRVGERMAAHGFAFEDSDEDIHMAIERLLGEEIGPLAGKLHT
                     GRSRNDQVATDVAMVVQNHSMEAIDLCAAAMERLLTLAESHQDWALPGYTHLQRAQPV
                     YLGHHLLAYFWMLARDVLRFQFALDSSSVMPLGSGALAGVNWEIDREAVAEDLGFDHV
                     THNSIDGASNRDFVLDYLSAASTCSMHLSRLGSEIVIWSSSEFGFCELDESFSSGSSI
                     MPQKKNPDSAELLRAKSPRVAASFQTLLGVMHALPLTYGKDMQEDKEPLFDAIDTIEG
                     LLDATAGMLEGIAFDRERMEAASGDEMLAATEVADLLVRRGMPFREAHGVVGGLVRDA
                     VEGGKSLSELTPVELKQRSELLDETYYEVLQRGSWLESKRIAGGTGSAPLAAQIQAAR
                     ETLAAVKARVAEERAE"
     gene            complement(1826..2965)
                     /locus_tag="VFB52_06360"
     CDS             complement(1826..2965)
                     /locus_tag="VFB52_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZO05990.1"
                     /translation="MSADLPPVPLAQPEIGEREETLVLEVLRSGQLSLGEVGVRFERE
                     LAAWLGVADAVSTSSGTTALHLGVRQLGWGPGDEVLTSPFSFVASANCLLYEGARPVF
                     CDVDPVTLNLDPAAAAAAAGERTKGILPVHIFGFPAAMPELEALAAKHGLGILEDACE
                     ALGALDSEGRLVGTRGNFATFAYYANKQMTTGEGGTIVPTDAAAAARLRSERNQGRAV
                     DMGWLDHDRLGYNYRLSDVAAAIGIAQVEKLDSLLERRAAVAALYEEALAGVEGVATP
                     IAGRGQERRSWFVYTVRLADGVDRDATIARLAERGVASKAYLPCIHLFPHLRELGYRE
                     GQFPVAEAAAAHSLALPFFPAMREDQVARVCQELAAALGQGAGTR"
     gene            complement(2975..>3269)
                     /locus_tag="VFB52_06365"
     CDS             complement(2975..>3269)
                     /locus_tag="VFB52_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZO05991.1"
                     /translation="TAEGNGPVNALDRALRDAIADRYPHLADIELANYKVRILDEDHG
                     TGAVTRVLLDSADAEHEWGTTGVSENIIEASWQALVDSLEYAFQPRAAAAGRE"
BASE COUNT          453 a         1134 c         1210 g          472 t
ORIGIN      
        1 gaggtcgatc cccagggcct cggtcagctt gcccgggccc gagcagaggt cggcgtcggc
       61 cttgccgccc cgtcgcgtcc gcatctcctc caccccgtgg gtcggctcca gggcccgaat
      121 cagcaccgcc gccgcctccc cctcgggttc ggccaccgcg ttgaggaggc tgtggatccc
      181 ataggagagg tagacgtagg cgtagccagg cggcccgaag agcacccgag tccgctcggt
      241 caggccgacg taggcgtgac aggcggggtc gtcgcgctcg tagctctcgg tctcgacgat
      301 cgtgccgccg accccgtcga agaagagccg gcagccgatc aggtcgcggg cgacctcgtg
      361 cacggaccgg tcgtagaagc ccgccgtcag ccgctcgccc atcggggcga cgttactcgg
      421 cccgctcctc ggcaacccgc gccttgaccg cggccagggt ctcccgcgct gcctggatct
      481 gcgccgccag cggggccgag ccggtaccgc cggcgatccg cttcgactcc agccagctgc
      541 cccgctgcag cacctcgtag taggtctcgt cgagcagctc cgagcgctgc ttcagctcaa
      601 ccggggtcag ctccgagagc gacttgccgc cttcgaccgc gtcgcggacg agcccgccga
      661 cgaccccatg cgcctcacgg aagggcatcc cgcggcggac cagcaggtcg gcgacctcgg
      721 tcgccgccag catctcgtcg ccggaggcag cttccatccg ttcacggtcg aaggcgatcc
      781 cctcgagcat cccggcggtc gcgtcgagca ggccctcgat cgtgtcgatc gcgtcgaaca
      841 gcggctcctt gtcctcctgc atgtccttgc cgtaggtgag cggcagcgcg tgcatcaccc
      901 cgagcagggt ctggaaggag gcggcgacgc gcgggctctt cgcccgcagc agctcggccg
      961 agtccgggtt cttcttctgc ggcatgatcg aggagcccga ggagaaggac tcgtcgagct
     1021 cgcagaagcc gaactcactg gagctccaga tgacgatttc cgagcccagc cgcgagaggt
     1081 gcatggagca ggtcgaggcg gcgctgaggt agtcgaggac gaagtcgcgg ttggaggcgc
     1141 cgtcgatcga gttgtgggtg acgtggtcga agccgaggtc ctcggcgacc gcctcgcggt
     1201 cgatctccca gttgaccccg gcgagcgcgc cggagccgag cggcatcacg ctggagctgt
     1261 cgagcgcgaa ctggaagcgc agcacgtcgc gggcgagcat ccagaagtag gcgagcaggt
     1321 ggtgcccgag gtagaccggc tgggcgcgct gcaggtgcgt gtagccgggc agcgcccagt
     1381 cctgatggga ctcggccagg gtcaggaggc gctccatcgc ggcggcgcag aggtcgattg
     1441 cctccatcga gtggttctgg acgaccatcg cgacgtcggt cgcgacctgg tcgttgcgcg
     1501 agcggccggt gtggagcttg ccggccaacg ggccgatctc ctcgccgagc agccgctcga
     1561 tcgccatgtg gatgtcctcg tcggagtcct cgaaggcgaa gccgtgcgcg gccatgcgct
     1621 cgccgacccg gtcgaggccg gcttccatct gtgccagctc gtcggcgtcg aggacgccga
     1681 tccgctgcag gccacgggcg tgggcgcgcg actggtcgag gtcgtacggc gccagccact
     1741 tgtcgaactc gagcgagcgg ttgaggcgcc agaagcgcgg gtcgggctct ttgcggagtc
     1801 tctgccggga catcggagcg ggcgattatc gcgtgccggc gccctgaccg agggccgccg
     1861 ccagctcctg gcagacgcgt gccacctggt cctcgcgcat cgcggggaag aacggcagcg
     1921 ccaacgagtg cgcggcggcc gcttcggcga ccgggaactg accctcgcgg tagcccaact
     1981 cgcgcaggtg gggaaagagg tggatacagg gcaggtaggc cttgctggcg accccgcgct
     2041 cggcgagccg ggcgatcgtc gcgtcgcggt cgacgccgtc ggcgagccgc acggtgtaga
     2101 cgaaccagct gcgccgctcc tggccgcggc cggcaatcgg ggtcgcgacc ccctcgaccc
     2161 cggccagcgc ttcctcgtag agggccgcaa ccgcggcacg gcgctccagc agcgagtcga
     2221 gcttctcgac ctgggcgatg ccgatcgcgg cggcgacgtc ggagaggcgg tagttgtagc
     2281 cgagccggtc gtggtcgagc cagcccatgt cgaccgcgcg gccctggttg cgctcgctgc
     2341 gcaggcgggc ggcggccgcg gcgtcggtgg ggacgatcgt gcccccctcc cccgtcgtca
     2401 tctgcttgtt cgcgtagtag gcgaaggtgg cgaagttgcc gcgggtgccg accagcctgc
     2461 cctcggagtc gagcgcgccg agcgcctcgc aggcgtcctc gaggatcccc aggccgtgct
     2521 tggcggcgag cgcctccagc tcgggcatcg ccgccgggaa gccgaagatg tggaccggca
     2581 ggattccctt ggtccgctcc cccgccgcgg ccgcggccgc cgccgggtcg aggttgaggg
     2641 tgaccgggtc gacgtcgcag aagaccggcc gcgccccctc gtagagcagg cagttggcgg
     2701 aggcgacgaa gctgaacggg ctggtcagga cctcgtcgcc ggggccccag ccgagctggc
     2761 gcacgccgag gtgcagcgcc gtggtgccac tggaggtcga gaccgcgtct gcgaccccga
     2821 gccaggcagc gagctcgcgc tcgaagcgca cgccgacctc gccgagcgag agctggcccg
     2881 agcgcagcac ctcgagcacc agggtctcct cgcgttcgcc gatctccggc tgggcgagcg
     2941 gaaccggggg cagatcggcg ctcatcgccg cagcctattc gcgcccggcg gcggccgcgc
     3001 gcggctggaa ggcgtactcg agcgagtcga cgagggcctg ccaggaggcc tcgatgatgt
     3061 tctccgagac cccggtcgtg ccccattcgt gctccgcgtc ggcggagtcg agcagcaccc
     3121 gggtgaccgc gccggtgccg tggtcttcgt cgaggatgcg gaccttgtag ttggccagct
     3181 cgatgtcggc gaggtgcggg tagcggtcgg cgatcgcgtc gcgcagggcg cggtcgagcg
     3241 cgttgaccgg gccgttgccc tcggcggtg
//
LOCUS       DASRIG010000231         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_886826, whole genome shotgun sequence.
ACCESSION   DASRIG010000231 DASRIG010000000
VERSION     DASRIG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886826"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..740
                     /gene="aroH"
                     /locus_tag="VFB52_06370"
     CDS             159..740
                     /gene="aroH"
                     /locus_tag="VFB52_06370"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZO05992.1"
                     /translation="MEAPRFDCAADPCLTQPNAAQLAARYDAVLPVGELRQTRANSGV
                     SSARYAGISHTGTEFAPGFGIMGRMETEQRLFAVRGATQVEANEADAILAATEELMKE
                     LISRNGLTAEAMVSCLFTTTEDLDAEFPAVAARRIGLDQVPLLCCREIPVPGSMPRVI
                     RVMAHYYAPVGHVPAHVYMGGAEDLRRDLQSAQ"
     gene            778..1872
                     /gene="hisC"
                     /locus_tag="VFB52_06375"
     CDS             778..1872
                     /gene="hisC"
                     /locus_tag="VFB52_06375"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZO05993.1"
                     /translation="MTVTFAEKLARMPGYQAGVPTGAAPESIAEAGIAQLASNESSFP
                     PHPAVVEAIQRAAGAMNRYPDPDATLLRRRLAERYETEPGRIAVANGSCEILLAAAEA
                     LAEPGAEIVYAWPSFSMYPYLPALTGAREIRVPLAPGDVHDLEAMANEVTAATQLVIV
                     CNPNNPTATHIPAAEIAEFCERIPTHVTVILDEAYIEFQTNDDPDATLELLPNFPNLV
                     LLRTFSKCYGLAGLRVGYSIGSAGFRAAVDAVRQPFSVNALAQAAGAEAILHQDDVAH
                     RVDATVAERLRVEEALAELGLETSESQANFSWIDLGIEDESGLVAGLGERLIAVRAGT
                     PLGDPGHIRVTYGTPEENDRFLTALDELLD"
     gene            2155..3258
                     /locus_tag="VFB52_06380"
     CDS             2155..3258
                     /locus_tag="VFB52_06380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZO05994.1"
                     /translation="MEKGSGKMHIGMQEGAKRDRVRDQRIAKVVELSPPERLLEELPL
                     GSERAKAVVRGREDVVEVLHGADPRLMVVVGPCSVHDPKAAMEYATKLAALSKQLQDD
                     LLIVMRVYFEKPRTTTGWKGLINDPHLDGSGDVNHGLRIARELMLKVVGTGLPVGCEF
                     LDPIIPQYISDTVAWGAIGARTTESQTHRQLGSGLSMPIGFKNRTDGNVQVAVDACRA
                     AAASHAFAGIDDAGMPAILHTTGNPDCHIILRGGHGQPNYFVPDVEDALVTLGVAGLP
                     ERLVIDASHDNSAKDPEKQFFAAGEIADQIADGNEAIVGVMLESFLVEGRQDLVPGEE
                     LVYGQSVTDPCIDWEDTVSVLERFASAVQARRG"
     gene            3255..4355
                     /locus_tag="VFB52_06385"
     CDS             3255..4355
                     /locus_tag="VFB52_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZO05995.1"
                     /translation="MSEEAPRVAVLGVGLIGGSIGLAARRRLGAEVVGYGRTPATLER
                     AVELGALDRAAGSLAEACADADLVFCCGPVQALPAQGGEALVACGPDAVVTDVGSTKG
                     ELVAALGEDERFIGGHPLAGAETAGVENAREDLFDGARWYLTPTERSSGLLYDRLQRT
                     VSALGARPQAVDPRTHDRLMATVSHVPHVLANALASEGAEALTRDSERLPEVGPSFRD
                     ATRVAGSNPAIWADIFATNREAVADSVESVARRLTAAAELIRAGEPDAIGAWHAAAGE
                     DRRRLHESESDAGPLRELRIVVANRPGTIAELALALGEAGVNIEDMALYPAPDMTSGA
                     VSLWVGGEEQATKAAELVRGLGHTVSVLADGG"
     gene            4368..5657
                     /gene="aroA"
                     /locus_tag="VFB52_06390"
     CDS             4368..5657
                     /gene="aroA"
                     /locus_tag="VFB52_06390"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZO05996.1"
                     /translation="MATRFDPAGPLRGNLRPPADKSISHRAALIAAMGEGTTRIDGYL
                     DAADTRSTLSAVEALGARVETADGLASLLYVEGVGLRGAGPATIDVGNAGTLLRLLPG
                     WLAGQAGESWTLDGDDSIRRRPVDRIAAPLRQMGARLAAREDRLPPLQVTGADLHGFT
                     YELPIASAQVKSCLLFAGLLADSETTIVEPDPSRDHTERMLAAAGAELSRAGNAVTIR
                     PAARLEPGAIVVPADISSAAFFVAAASLVAGSEVVLEAVGVNPTRTGILTILERMGAA
                     IEVEPGGESGGEPIAALRVRPAPLRATEIGGAEIPLAIDELPLVALAACFAEGTTTIR
                     DAEELRRKESDRVETVTAALSALGGRVEATPDGMVIEGSGGLRGGTIASHGDHRIAML
                     GAVAGLASAEGVEVEGMDAAAVSYPGFERDLASLLRG"
     gene            5675..6313
                     /gene="cmk"
                     /locus_tag="VFB52_06395"
     CDS             5675..6313
                     /gene="cmk"
                     /locus_tag="VFB52_06395"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZO05997.1"
                     /translation="MVIAIDGPAGAGKSSVARAVASELGITYLDSGAMYRCVALAALD
                     TGADLDDGAALGTLAAGLEIEFDGRRVLLGGDDVSAAIRAPRVSAAASRVSVHPGVRE
                     AMVARQQALIATADFVAEGRDIGTVVSPDSPLKVFLTASDEERARRRAAETGEDFESV
                     LDAQRRRDARDTDREHGALRPADDAVELDTTGLGLEEVVGRVVSLARERGLP"
     gene            6310..7635
                     /gene="der"
                     /locus_tag="VFB52_06400"
     CDS             6310..7635
                     /gene="der"
                     /locus_tag="VFB52_06400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZO05998.1"
                     /translation="MSAVTPKVAIVGFPNVGKSTLINRLVGGTEAVTAAEPGVTRDRK
                     RLATEWNGVAFELIDTGGIDLEDEAELARDIQAQARLAIAEADAILMVVDGRAGLRAG
                     DAELAKTLRGGKVPVLVVVNKVDHPHDYSATAEFHALGLGEPLAVSATHGLGTGDLLD
                     AIVTALGEREPREEPEDAVRVAVIGRPNVGKSSLVNSFLGADRVIVSDIAGTTRDAID
                     TELEVDGRRVILVDTAGLRRRAKVAGTVDYYAQLRSERAVERADVALVVCDASEGVTS
                     EDLRVAEMSMKAGCATMLVLNKWDVGETDIDDARARVERKSRLRPAVITASALRGRNV
                     PALLPKALELADRAGTRVPTAELNRFVSAVVAKTPPPARRGRRLKLLYAAQVGERPPR
                     IAIQVNDRKLITRDWAYHLENRMRETYELEGVPLIIDFVPRTGYRGRQD"
     gene            7674..>8426
                     /locus_tag="VFB52_06405"
     CDS             7674..>8426
                     /locus_tag="VFB52_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZO05999.1"
                     /translation="MPTKPLAENEHLFTSESVTEGHPDKVADAISDGVLDAIMAEDPT
                     GRVACETLVNTGMAVVSGEITTDVHLDIPEIVRETVREIGYTGSGYGYDCDHISVLVS
                     LDKQSPDIAQGVDVAYETRVEDADDELLIAGAGDQGMMFGYASDETEALMPMPIHIAH
                     RLAERLAAVRKDGTLGYLGPDGKTQVTVRYREGKPVSIDKLLISTQHDAGVDSLTQIK
                     PDLFEHVVLPVLAAEVPGRYDEAELLDGFLVNP"
BASE COUNT         1231 a         3104 c         2944 g         1147 t
ORIGIN      
        1 tcgaccaccg ccggtgcgcg atccagtagg ccgtggagcg cggcagcgct gcggtgagac
       61 accacggtcc cgtgcccaca ggccaacacc gccgccatca gtcgccctcg atcgcttatt
      121 cgcggacgcc cgaccgcgta gatgccgcgg aacacgcgat ggaggcgccc cgattcgatt
      181 gcgcggccga tccctgcctc actcagccca atgcggcgca gctggcggcg agatacgacg
      241 ccgtgctgcc tgtcggcgag ctgcgccaga cgcgcgccaa ttccggtgtg agttctgctc
      301 gctatgcggg cataagtcac accggaaccg aattcgcccc cggctttggg attatggggc
      361 gcatggagac ggagcagagg ctttttgcgg tccggggggc gacacaggtc gaggccaatg
      421 aggcggacgc gatcctggcg gcgaccgagg agttgatgaa ggagctgatc tcgcgcaacg
      481 gcctcacggc ggaggcgatg gtcagctgcc tgttcacgac caccgaggat ctcgatgccg
      541 agttcccggc ggtggcggcg cggcggatcg ggctcgacca ggtgccgttg ctctgctgtc
      601 gcgagatccc ggttccgggc tcaatgccgc gggtgatccg ggtgatggcg cactactacg
      661 cgccggttgg gcatgtgccc gcgcacgtct acatgggcgg tgccgaggac ctgcgacgcg
      721 acctgcagtc ggcgcagtaa caccgtcgcc gctgccgctg cgtcagaatt gccggcgatg
      781 accgtcacct tcgctgagaa gctcgcccgc atgcccggct accaggccgg cgtgccgact
      841 ggcgctgccc cggagtcaat cgccgaggcc gggatcgccc agctcgcctc caacgagtcc
      901 tcgttcccgc cgcacccggc ggtggtcgag gcgatccagc gcgccgccgg ggcgatgaac
      961 cgctacccgg accccgacgc gaccctgctg cgccgccgtc tcgccgagcg ctacgagacc
     1021 gagccgggcc ggatcgccgt cgccaacggc tcctgcgaga tcctgctcgc ggccgccgag
     1081 gcgctggccg agcccggcgc cgagatcgtc tacgcctggc cctccttctc gatgtatccg
     1141 tacctgccgg ccctgaccgg cgcccgcgag atccgcgtgc cgctcgcccc cggcgacgtc
     1201 cacgacctcg aggcgatggc caacgaggtg accgcggcga cccagctggt gatcgtctgc
     1261 aacccgaaca acccgaccgc gacccacatc cccgcggccg agatcgccga gttctgcgag
     1321 cggatcccga cgcacgtgac ggtgatcctc gacgaggcct atatcgagtt ccagaccaac
     1381 gacgaccccg acgcgaccct cgaactgctg ccgaacttcc ccaacctggt cctcctgcgc
     1441 accttcagca agtgctacgg cctcgccggg ctgcgggtcg gctactcgat cggctccgcc
     1501 ggcttccgcg ccgcggttga cgcggtgcgc cagccgttca gcgtcaacgc gctcgcccag
     1561 gcggccgggg cggaggcgat cctgcaccag gacgacgtcg cccaccgggt cgatgccacc
     1621 gtcgccgagc gcctgcgggt cgaggaggcc ctcgccgagc ttggcctcga gacctccgag
     1681 tcgcaggcca acttctcctg gatcgacctc gggatcgagg acgagagcgg cctcgtcgcc
     1741 ggcctcggcg agcgcctgat cgcggtccgt gccggtaccc cgcttggtga tcccggccac
     1801 atccgcgtca cctacggcac ccccgaggag aacgaccgct tcctcaccgc gctcgacgag
     1861 ctgctcgact gagccgaccc cccggattgc tgcaccgcgc tcgactaaat ccgggcgccg
     1921 tgctacaaac aaagccaagg tgaatcggaa gcgcacatct gcaaacggcc ccagcgccct
     1981 cggcgccgcc gttgcccctg cgttcttcac ctattggcga tttagctagg cgcccggcct
     2041 ggcggcatct cgccgcgaca aaaaggagtt tctcggcccg ggcgccgaac ccaagtttcc
     2101 gcgtcggccg cggttttttg gcggttggcg accagtaagt acgatttcag cggtatggag
     2161 aaaggctcag gcaaaatgca catcgggatg caggagggcg cgaaacggga tcgagtcagg
     2221 gatcagcgaa tcgccaaagt ggtggagctg tctccaccgg aacgattgct ggaggaactt
     2281 ccgcttggct cggagcgggc aaaagcggtg gtgcgcggcc gcgaagacgt ggtcgaggtg
     2341 ctgcacgggg cggacccgcg gctgatggtc gtggtcgggc cctgcagcgt gcacgacccg
     2401 aaagcggcga tggagtacgc gacgaagttg gcggcgctct ccaaacagct tcaggacgac
     2461 ctgctgatcg tgatgcgggt ctacttcgag aagccgcgga cgacgaccgg ctggaagggg
     2521 ctgatcaacg atccgcacct ggacggctcc ggcgacgtca accacggcct gcggatcgcc
     2581 cgcgagctga tgctgaaggt ggtcgggacc gggctgccgg tcggctgcga gttcctcgac
     2641 ccgatcatcc cgcagtacat ctcggacacg gtcgcctggg gcgcgatcgg cgcgcgcacg
     2701 accgagagcc agacgcaccg ccagctcggc tccggcctct cgatgccgat cgggttcaag
     2761 aaccgcaccg acggcaacgt ccaggtcgcg gtcgacgcct gtcgtgctgc cgcggcgtcc
     2821 cacgccttcg ccgggatcga cgacgcgggc atgccggcga tcctgcacac gaccggcaac
     2881 cccgactgcc acatcatcct gcgcggcggt cacggtcagc cgaactactt cgtgccggac
     2941 gtcgaggacg ccttggtcac gctcggcgtc gccgggctgc cggagcggtt ggtgatcgac
     3001 gcctcccacg acaacagcgc caaggacccc gaaaagcagt tcttcgcggc cggtgaaatc
     3061 gccgaccaga tcgccgacgg caacgaggcg atcgtcgggg tgatgctgga gtccttcctg
     3121 gtcgagggtc gccaggacct ggtcccgggc gaggagctgg tctacggcca gtcggtcacc
     3181 gacccctgca tcgactggga ggacacggtc tccgtgctcg agcggttcgc ctccgcggtc
     3241 caggcccggc gcggttgagc gaggaggcgc ccagagtcgc cgtcctcggc gtcggcctga
     3301 tcggtggctc aatcggtttg gcggcgcggc ggcggctcgg ggccgaggtc gtcggctacg
     3361 ggcgcacgcc ggcgacgctg gagcgcgcgg ttgagctcgg ggcgctggac cgcgccgccg
     3421 gctcgctggc cgaggcctgc gctgacgccg acctcgtctt ctgctgcgga cccgtgcagg
     3481 cgctgccggc gcagggcggc gaagcgctgg tcgcctgcgg ccccgacgcg gtcgtcaccg
     3541 acgtcggctc gaccaagggc gagctggtcg cggccctcgg cgaggacgag cgcttcatcg
     3601 gcggtcaccc gctggccggg gccgagaccg ccggggtcga gaacgcccgc gaggacctct
     3661 tcgacggcgc ccgctggtac ctgaccccga cagagcgctc cagcggcttg ctctacgacc
     3721 gcctgcagcg cacggtcagc gccctcggcg cccgcccgca ggcggtcgac ccacgcaccc
     3781 acgaccgtct gatggcgacg gtcagccacg tcccgcacgt gctcgccaac gcgctcgcct
     3841 ccgagggcgc cgaggcgctg acccgcgact ccgagcgcct gcccgaggtc gggccgagct
     3901 tccgcgacgc gacccgtgtc gccggctcca acccggcgat ctgggcagac atcttcgcca
     3961 ccaaccgcga ggcggtcgcc gactcggtcg agtcggttgc ccgccggctc accgcggccg
     4021 ccgagctgat ccgcgccggc gagccggacg cgatcggcgc ttggcacgcc gccgccggcg
     4081 aggaccgccg ccgcctgcac gagtccgagt ccgacgccgg cccgctgcgc gagctgcgga
     4141 tcgtcgtcgc caaccggccg ggcacgatcg ccgagctggc cctggcgctg ggcgaggccg
     4201 gggtcaacat cgaggacatg gcgctctacc cggcgccgga tatgacctcg ggcgcggtct
     4261 cgctctgggt cggcggcgag gagcaggcga cgaaggcggc ggagctggtc cgcggcctcg
     4321 gccacacggt gagcgtgctc gccgacggcg ggtagtctcg cgggcgaatg gcgacccgct
     4381 tcgaccctgc cggacccctg cgcggcaacc tccgcccgcc cgccgacaag tcgatctccc
     4441 accgcgccgc gctgatcgcg gcgatgggcg aggggacgac gcggatcgac ggctacctcg
     4501 atgccgctga cacgcgctcg accctgagcg cggtcgaggc gctgggggcg cgggtcgaga
     4561 cagccgacgg gctcgcctcg ctgctgtacg tcgagggcgt cggcctgcgc ggcgccggcc
     4621 cggcgacgat cgacgtcggc aacgccggca cgctgctgcg cctgctgccg ggctggctgg
     4681 cggggcaggc gggggagagc tggacgctcg atggcgacga ctcgattcgc cgccgcccgg
     4741 tcgaccggat cgccgcgccg ctgcgccaga tgggggcccg gctcgccgcc cgcgaggacc
     4801 ggctgccgcc gctgcaggtg accggcgccg acctgcacgg gttcacctac gagctgccaa
     4861 tcgccagcgc ccaggtcaag tcttgtctgc tatttgcagg gcttttggcc gactcggaga
     4921 cgaccatcgt cgagccggac ccgagccgcg accacaccga gcggatgctc gcggcggccg
     4981 gcgccgagct ctcgcgcgcg ggcaacgcgg tgacgatccg gcccgccgcg cggctcgaac
     5041 cgggcgcgat cgtcgtcccc gccgacatct cctcggcggc cttcttcgtc gccgccgcga
     5101 gcctggtcgc cggcagcgag gtcgtgctcg aggcggtcgg ggtgaacccg acccggaccg
     5161 ggatcctcac gatcctcgag cggatgggcg ccgcgatcga ggtcgagccg ggaggcgaga
     5221 gcggcggcga gccgatcgcc gcgctgcgcg tgcgcccggc gccgctgcgg gcgaccgaga
     5281 tcggcggcgc cgagatcccc ctggcgatcg acgagctgcc gctggtcgcg ctcgccgcct
     5341 gcttcgccga gggcacgacg acgatccgcg acgccgagga gctgcgccgc aaggagtccg
     5401 accgggtcga gaccgtgacc gcggcgctga gcgcgctcgg cggcagggtc gaggcgacgc
     5461 ccgacgggat ggtgatcgag ggcagcggcg ggctgcgcgg cggcacgatc gcctcgcacg
     5521 gcgaccaccg gatcgcgatg ctcggcgccg tcgccggcct cgccagcgcc gagggggtcg
     5581 aggtcgaggg catggacgcc gccgcggtca gctatcccgg cttcgagcgg gacctggctt
     5641 cgctgctgcg cggctgagcc atacgctgcg ccccatggtc atcgccatcg acggtcccgc
     5701 cggggccggc aagtcatcgg tcgcgcgcgc ggtcgcctct gagctcggga tcacctacct
     5761 cgactccggg gcgatgtatc gctgcgtggc gctggcggcg ctcgacaccg gggccgacct
     5821 cgacgacggc gccgcgctcg gcacgctggc ggccggcctc gagatcgagt tcgacggccg
     5881 ccgcgtgctg ctcggcggcg acgacgtcag cgccgcgatc cgcgccccgc gggtcagcgc
     5941 cgcggcctcg cgggtctcgg tccacccggg tgtacgcgag gcgatggtcg cccgccagca
     6001 ggcgctgatc gcgaccgccg acttcgtcgc cgagggtcgc gacatcggca ccgtggtcag
     6061 ccccgactcg ccgctgaagg tcttcctcac cgcctccgac gaggagcggg cgcgccgccg
     6121 cgccgccgag accggcgagg acttcgaatc cgtgctcgac gcccagcgcc gccgcgacgc
     6181 ccgcgacacc gatcgcgagc acggcgccct gcgtcccgcc gacgacgccg tcgagctgga
     6241 cacgacaggg ctcggcctcg aagaggtcgt cggccgcgtc gtctccctcg cccgcgagcg
     6301 gggcctgccg tgagcgcggt gacgcccaag gttgcgatcg tcggcttccc caacgtcggc
     6361 aagagcaccc tgatcaaccg cctggtcggc ggcaccgagg cggtgaccgc ggccgagccg
     6421 ggggtgaccc gcgaccgcaa gcggctcgcc accgagtgga acggggtcgc cttcgagctg
     6481 atcgacaccg gcggcatcga cctcgaggac gaagccgagc tggcccgcga catccaggcg
     6541 caggcgcgcc tcgcgatcgc tgaggccgac gcgatcctga tggtcgtcga cggccgtgcc
     6601 gggttgcgcg ccggcgacgc cgagctggcg aagacgctgc gcggcggcaa ggtgccggtg
     6661 ctggtcgtcg tcaacaaagt cgaccacccc cacgactact cggcgaccgc cgagttccac
     6721 gcgctcgggc tcggtgagcc gcttgccgtc tccgccaccc acggcctcgg taccggcgac
     6781 ctgctcgacg cgatcgtcac cgcgctcggt gagcgcgagc cccgcgagga gcccgaggac
     6841 gccgtccgcg tcgccgtgat cggccgcccg aacgtcggca agtcctcgct cgtcaactcc
     6901 ttcctcggcg ccgaccgggt gatcgtctcc gacatcgccg gcaccacccg cgacgcgatc
     6961 gacaccgagc tcgaggtcga cggccgccgc gtgatcctgg tcgacaccgc cggcctgcgc
     7021 cgccgcgcca aggttgcggg cacggtcgac tactacgccc agctgcgctc cgaacgggcg
     7081 gtcgagcggg ccgacgtcgc cttggtcgtc tgcgacgcct ccgagggggt cacctcggag
     7141 gacctgcggg tcgccgagat gtcgatgaaa gccggctgcg cgacgatgct ggtgctcaac
     7201 aagtgggacg tgggggagac cgacatcgat gacgcccgcg cccgggtcga gcgcaagtcg
     7261 cgcctgcgcc cggcggtgat caccgcctcg gccctgcgcg gccgcaacgt ccccgccctg
     7321 ctgccgaagg cgctggagct cgccgaccgg gccggcaccc gcgtcccgac tgccgagctg
     7381 aaccgcttcg tcagcgccgt ggtcgcgaaa acgccgcccc cggcgcgtcg cggccggcgc
     7441 ctcaaactgc tctacgccgc ccaggtcggt gagcgcccgc cgcgcatcgc gatccaggtc
     7501 aacgaccgca agctgatcac ccgcgactgg gcctaccacc tggagaaccg gatgcgcgag
     7561 acctacgagc tggagggggt cccgctgatc atcgacttcg tccccagaac cggctatcgc
     7621 ggccgtcagg actgaccctc ggggacttgc cgctttggtc taggcttccg cgaatgccaa
     7681 ccaagccgct cgccgagaac gagcacctct tcacgtccga gtccgtcacc gagggtcatc
     7741 ccgacaaggt tgcggatgcg atttccgacg gcgtcctcga cgcgatcatg gccgaggatc
     7801 cgaccggccg cgtcgcctgc gagacgctcg tcaacaccgg catggcggtg gtctcgggcg
     7861 agatcaccac cgacgtccac ctggacatcc cggagatcgt ccgcgagacg gttcgcgaga
     7921 tcggctacac cggtagcggc tatggctacg actgcgacca catctcggtc ctggtctccc
     7981 tcgacaagca gtcgcccgac atcgcccagg gcgtcgacgt cgcctacgag acccgcgttg
     8041 aggacgccga cgacgagctg ctgatcgccg gagccggcga ccagggcatg atgttcggct
     8101 acgccagcga cgagaccgag gcgctgatgc cgatgccgat ccacatcgcc caccggctcg
     8161 ccgagcggct cgcggcggtg cgcaaggacg gcaccctcgg ctacctcggc cccgacggca
     8221 agacccaggt gacggtccgc taccgcgaag gcaagccggt ctcgatcgac aaacttctga
     8281 tctcgaccca gcacgacgcg ggcgtcgact cgctcaccca gatcaaaccc gacctcttcg
     8341 agcacgtcgt cctcccggtc ctcgcggccg aggtccccgg ccgctacgac gaggccgaac
     8401 tgctggacgg cttcctggtc aacccg
//
LOCUS       DASRIG010000232         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3499665, whole genome shotgun sequence.
ACCESSION   DASRIG010000232 DASRIG010000000
VERSION     DASRIG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499665"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /locus_tag="VFB52_06410"
     CDS             complement(<1..3026)
                     /locus_tag="VFB52_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZO06000.1"
                     /translation="MRRTLLIAIVVALALLACASAALASELKVDTLEDRTGGSCSEVS
                     SCSLREAIAAVNAGQVTGDVKIAIDVEGTIGIGDKGALKIDAPLAVSSVWIAGPTAEA
                     SDLTISGNEKSRILELEAGEVQLTDMTLKEGHVTSFAVDGDGGGAIYQEGGVLVLKDV
                     RLTENKVASGRDGGAIAIEGPGVLTLIDSLVDHNTAEKPTNAQGNGGGIYQDAALSLL
                     TLEDTEVFANNAEEGQGGGINQFEGNLSITGGRIAGNNATGGGGGISTDADGTHTTTI
                     VESSIVENTAVDEGGGIWTDNGSGGVTIEKTAIEKNSTEVRGAGVYVDGPTTIVDSTV
                     AHNEFKPGGEDDHGVGIEVWDDKLTLENSTVAMNAGVGLYVESGAEASVHASTIAANT
                     TNDEAAGGLAGDGDFELASTIVAYNVADGQTADCDAEVTSLGHNVLGKAAGCEWTAGS
                     GDQTNVLPGLGSLQDNGGPTETMAPVSFGSVAINHGFEPASTDQRGKTRPIPDSPAFT
                     DVGAVEVQAPVNTELPWIDPSGAITPGTELLCEMGTWDPDTVENPQVRYDWFVEGEVV
                     AEDTDTYETTAADAGKDITCEVAMNNGATVTEAISEPVHFGPATASLSPAVLDFGERE
                     VDSGEPPAEQTLTLKNKGGEDLTVSSIEGNSSNLFPLDLSDCNGGSIVLASGESCEIG
                     VGFEPNADGPRSSTITVQSNGGNPTAEAKGTGLAPVLTITPNFHDFGEVAVGTGPGAW
                     FPFSVKNVGGADGPVFISEFEGDEFALDDDGCDGVELEPGQECTIEVAFRAEAPGAFS
                     GELGVSARNEGSEAIATLSGTATQAVLSASPAELDFKPQLVGTMTTQTVTISNSGTGT
                     AKVGSVALEGPGTSSFDLPAGGEECTGAELAGSETCTVEVAFKPIAAGLVSVNLQLEG
                     DATTPTTVLIGGTGTEPPPPAEASAALTAPGPLTGNAAGELPIGVACAATGPDCQVTL
                     ALQSGGKALGSWSGTLAPGASQTAEVPLSPAARKQLG"
     gene            complement(3029..>3532)
                     /locus_tag="VFB52_06415"
     CDS             complement(3029..>3532)
                     /locus_tag="VFB52_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06001.1"
                     /translation="SGGKALGGWSGTLAPGAAATAKVPLSSAARKQLGAKGKLAAEAV
                     LNVAGGKGSTTAIALLAPPAPKLKLKSAKRAGNAIVFKLTCQGYAAKCQGKLALSAAK
                     GGAKVAAGKVSLAKGTHEVRVPLTGAGKRLLDDEPRAQLKAEVSAKDPVYQRTTVAKS
                     RLRLGGK"
BASE COUNT          410 a         1159 c         1288 g          675 t
ORIGIN      
        1 ccgagctgct tgcgggcagc ggggctgagc ggcacttcag ccgtctggct cgcgcccggg
       61 gcgagtgtgc cgctccagct gccgagggct ttgccgcccg actgcagggc cagcgtcacc
      121 tggcagtccg gcccggtcgc agcgcaggcg accccgatcg gcagctcacc ggcggcgttg
      181 ccggtgagcg ggccgggagc ggtgagcgcg gcgctcgcct cggccggcgg gggcggctcc
      241 gtcccggtgc ccccgatcag caccgtggtg ggcgtggtgg cgtcgccttc gagctggaga
      301 ttgacggaga ccaggccggc ggcgatcggc ttgaacgcaa cttccaccgt gcaggtctcg
      361 ctcccggcga gttcggctcc ggtgcactcc tcgcccccgg cgggcaggtc gaaggaggag
      421 gtaccgggcc cttcgagcgc gaccgatccg actttggcgg tgccggtgcc ggagttggag
      481 atcgtcaccg tctgcgtcgt catcgtgccg accagctgcg gtttgaagtc gagttcggcg
      541 gggctggcgg agaggacggc ctgggtggcg gttccggaga gggtcgcgat cgcttcgctg
      601 ccctcgttcc gggcggagac gccgagttcg ccgctgaacg cgccgggcgc ttccgcccgg
      661 aaggcgactt cgatcgtgca ttcctggccg ggttcgagct cgacgccgtc gcaaccgtcg
      721 tcgtcgagcg cgaattcgtc gccttcgaac tcggagatga agacgggacc gtcggcgccg
      781 ccgacgttct tcacgctgaa cgggaaccag gcgccggggc cagtaccgac cgcgacttcg
      841 ccgaagtcgt ggaagttggg ggtgatcgtg agcaccggcg ccaggccggt gcctttggct
      901 tcggcagtgg ggttgccgcc gttgctctgg acggtgatcg tcgacgaccg gggaccgtcg
      961 gcgttcggtt cgaagccgac gccgatctcg cacgactcgc cggaggcgag gacgatgctg
     1021 ccgccgttgc agtcgctgag atcgagcggg aacaggttgg agctgttgcc ttcgatcgaa
     1081 gacacggtga ggtcttcgcc gcctttgttc ttcagggtga gggtctgttc ggccggcggt
     1141 tcgccgctgt cgacttcgcg ctcgccgaag tcgagcacgg ccgggctgag cgaggcggtc
     1201 gcggggccga agtggaccgg ctcggagatc gcttcggtga ccgtggcgcc gttgttcatc
     1261 gccacctcgc aggtgatgtc cttgccggcg tctgccgcgg tcgtctcgta ggtatccgtg
     1321 tcctcggcca ccacctcgcc ctcgacgaac cagtcgtagc ggacctgcgg gttttcgacc
     1381 gtgtcgggat cccaggtgcc catctcgcag agcagctcgg ttcccggggt gatcgcgccg
     1441 ctgggatcga tccagggcag ctccgtgttg accggcgcct gcacctcgac cgcgccgacg
     1501 tcggtgaagg cggggctgtc ggggatcggg cgggtctttc cgcgctggtc ggtgctggcg
     1561 ggttcgaagc cgtggttgat cgcgacgctg ccgaagctga ccggcgccat cgtttcggtc
     1621 ggcccgccgt tgtcctgcag cgatccgaga ccggggagga cgttggtctg gtcgccgctg
     1681 cctgcggtcc attcgcagcc ggctgctttg ccgagcacgt tgtggccgag gctggtcact
     1741 tcggcgtcgc agtcggcggt ctggccgtcg gcgacgttgt aggcgacgat cgtgctcgcc
     1801 agctcgaagt cgccgtcgcc ggccagtccg ccggctgctt cgtcgttggt cgtgttggcg
     1861 gcgatcgtcg aggcgtggac cgaggcttcg gcgccgctct cgacgtagag cccgaccccc
     1921 gcgttcatcg cgaccgtcga gttttcgagc gtcagcttgt cgtcccagac ctcgatcccg
     1981 accccgtggt catcttcgcc gccgggtttg aattcgttgt gcgcgaccgt gctgtcgacg
     2041 atcgtggtcg ggccgtcgac gtagacgccg gcgccgcgca cctcggtgct gtttttttcg
     2101 atcgccgtct tttcgatcgt cacaccgccg gagccgttgt cggtccagat gccgccgccc
     2161 tcgtcgaccg cggtgttttc gacgatcgac gattcgacga tggtggtcgt gtgagtgccg
     2221 tccgcgtcgg tggagatccc gccgccgcca cccgtcgcgt tgttgccggc gatccggcca
     2281 ccggtgatcg agaggttgcc ttcgaactgg ttgatgccgc cgccctgccc ctcttcggcg
     2341 ttgttggcga agacttcggt gtcctccagg gtgagaaggc tgagcgcggc gtcctggtag
     2401 ataccgccgc cgttgccctg ggcgttggtc ggtttttcgg ccgtgttgtg gtcgaccagc
     2461 gagtcgatca gggtcaggac gccggggcct tcgatcgcga tcgcgccgcc gtcgcggccg
     2521 ctggcgacct tgttctcggt caggcgcacg tccttgagca cgagcacgcc gccttcctgg
     2581 tagatcgcgc cgccgccgtc gccgtcgacc gcgaagctgg tcacgtggcc ttccttcagg
     2641 gtcatgtcgg tgagctggac ttcgcccgct tcgagttcga ggatccgcga tttttcgttg
     2701 ccgctgatcg tcagatcgga agcttcggcg gtcgggccgg cgatccagac ggagctgacg
     2761 gcgagcggcg cgtcgatctt cagcgcgcct ttgtcgccga tcccgatcgt gccttcgacg
     2821 tcgatcgcga tcttcacgtc gccggtcacc tgaccggcgt tgaccgccgc gatcgcttca
     2881 cgcagcgagc aggagctgac ttcgctgcag ctgccgccgg tgcggtcttc gagcgtgtcg
     2941 actttcagtt cggaggcgag agcggcggag gcgcaggcga gcagggccag cgcgacgacg
     3001 atcgcgatca gcagggtgcg gcgcatcgct atttaccccc gaggcggagt cgggacttgg
     3061 cgacggtggt ccgctggtaa accgggtcct tggcactgac ctcggccttc agctgggcgc
     3121 gcggttcgtc gtcgagcagg cgcttgccgg cgccggtcag cggaacgcgg acctcgtgcg
     3181 tgcctttggc cagcgagacc ttgccggcgg cgaccttggc gccgcccttg gccgcgctca
     3241 gcgccagctt gccctggcat ttcgccgcgt agccctggca ggtcagcttg aagacgatcg
     3301 cgttgccggc ccgtttggcc gacttcagct tcagcttcgg ggccgggggc gcgagcaggg
     3361 cgatcgcggt ggtcgagcct ttgccgccgg cgacgttcag cacggcttcg gcggcgagct
     3421 tgcctttggc gccgagctgc ttgcgggcgg cgctgctcag cggcactttg gccgtcgcgg
     3481 cggcgcccgg ggcgagcgtg ccgctccagc cgccgagcgc tttgccgccc ga
//
LOCUS       DASRIG010000233         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3780442, whole genome shotgun sequence.
ACCESSION   DASRIG010000233 DASRIG010000000
VERSION     DASRIG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3780442"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFB52_06420"
     CDS             <1..562
                     /locus_tag="VFB52_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO06002.1"
                     /translation="ELVAQGKVAHLGLSEAAPETIRRAHATHPIAALQTEYSLWSREP
                     EDEILPTVRELGIGFVPYSPLGRGFLTGRFQSLDDLPEGDFRRVNPRFEGENFDRNLR
                     LVERVTEIAAEKGATPGQLALAWVLAQGEDVVPIPGTKRLSYLEQNVAAAEIELSEED
                     LRRIDEAAPADAAAGDRYADMSSVNG"
     gene            complement(552..1502)
                     /locus_tag="VFB52_06425"
     CDS             complement(552..1502)
                     /locus_tag="VFB52_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZO06003.1"
                     /translation="MPAPRRPKMPRDLFETRSHTWQALGLGDELTPRFSKKALGGLLA
                     ALVVIAATLVVYHQRAHIAPGYGEWIRIGTVVVLVIVGSAATHWLSKGLAPRMNRRLD
                     PATAGTLGFAIRLLAMSAVVILALRIAGVTASTLAVGGAFTAVVLGLAAQQSLGGIFA
                     GIVLQSTRPFRVGERVRLVGGALAGSLEGTVSSLGLFYTTLSQGADRLLVPNNVLLQL
                     TVVPLREPEKVDVRARFPTDASPQRVERRLLREITVPTRYRPSVSLEELDHDSVVLRV
                     NATPLHPEDGSQLAEEVLEALRSQERAETGEHPVANGASR"
     gene            complement(1574..1930)
                     /locus_tag="VFB52_06430"
     CDS             complement(1574..1930)
                     /locus_tag="VFB52_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZO06004.1"
                     /translation="MHPVPFETSSAEVGGVRVIAVRGELDLSTAPDLEGPLDAAVNGG
                     DAGVLIDLGECEFIDSTGIALIVRAWQQLSGNGGKGLAICSPSDQVKRVLDVSGLDQS
                     IPVHGSRDEGVAALRG"
     gene            1938..2912
                     /locus_tag="VFB52_06435"
     CDS             1938..2912
                     /locus_tag="VFB52_06435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO06005.1"
                     /translation="MLPQQTGEVIVCTRFAAAWPGVLGGPGSGASYARRRKPDLPRRP
                     GIGNLNTQTDDRPGLRLSLPARAENVAVVRHALAGLGERLGMDDTSLADLKTVVTEAA
                     MNVVVHAYPETEEGLLAVEAHGEHDGLTVVVRDFGSGIRPRPDPERPSLRIGLSLIAA
                     LSSSFEIKGGIDRGTEIRMHLPLRAASGDGRPELTQQWETPEATEVRVGPPELVGPVL
                     SRVLSALAARHEITIDRLSDTMLLSDAISAAAPQGFTDGHVELSIADRPEGVELRVGP
                     MTEGGANRLRKGLDLPQVGSLESLADDLRVEQGEEGEFLVVAIDSLAA"
     gene            complement(2927..3751)
                     /locus_tag="VFB52_06440"
     CDS             complement(2927..3751)
                     /locus_tag="VFB52_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZO06006.1"
                     /translation="MAANSENTPATSSPSIDEHATDGLASEQALWKRFSERRDPATRE
                     ELVKRYLPFAKSLALRYRGASESFDDLLQVASLGLVNAIDRFDPSRGTPFTAFASPTI
                     LGELKRHFRDRVWTVRVPRGLHDRMAEVEKAITELTVTLQRSPSVPEIAAKMEVDPSE
                     VLEVLEANHNRRPLSLDRPVGGEEDESPASEWVGDEDAGYELIEDKLALEGVLPQLDE
                     RERLVLQLRFVEDMTQSQIAEKIGHSQMHVSRILRRTLERIRAEVAEQNREAGREE"
     gene            4047..4724
                     /locus_tag="VFB52_06445"
     CDS             4047..4724
                     /locus_tag="VFB52_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HZO06007.1"
                     /translation="MQGKLDLSVEASVYDIDGFESSRADVRALQRRGRKVVCYLDVGS
                     WETYRPDAARFPRSVIGRKYDGFPDERWLDVSRFQLFAAPLRERIAMCARKGFDAVEP
                     DNLAGWEPENKTGFAITRADQLRFNRWIARQVHRHGMAVALKNDGRQAKQLVGDFDFA
                     IVEECFQYDECGFYKPFVERGKAVFVAEYELEPSEFCAAADDLEFSAIRKSYDLFARP
                     WRPCGSG"
     gene            4762..5157
                     /locus_tag="VFB52_06450"
     CDS             4762..5157
                     /locus_tag="VFB52_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06008.1"
                     /translation="MGFDQYHEPPEELSAETRTFARICASLEEESQAIGWYQQRISVE
                     ADPDARAIMEDAQGEEFKHFAMNLEFLLRRTPRWRAICEGVLFKDGDIVEHGEAAEAA
                     SEDGGDGGEDEAGGDDASLGIGGMKGAGQ"
     gene            5154..5957
                     /locus_tag="VFB52_06455"
     CDS             5154..5957
                     /locus_tag="VFB52_06455"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HZO06009.1"
                     /translation="MNHLLRGLAPVSEAGWQLLDDEARERLQGPLAARRMVDFSGPHG
                     WEHSAINLGRTEKLPPPNGKGVTALRRRVLPLAELRAGFTISRAELADNDRGAVDADL
                     AALDEAARQIAVAENVAVFHGWKKAGITGITEASPFKPEKLGKTAETYPRCVARAVES
                     IRQAGVEGPYGLALGPDEYTRVIETAEHGGYPLFDHLAKILGGPIVWAPGVKGAVVLS
                     LRGGDFLFESGQDLAIGYASHDAANVELYLEESFSFVVATPEAAAPLSS"
     gene            complement(5972..>6401)
                     /locus_tag="VFB52_06460"
     CDS             complement(5972..>6401)
                     /locus_tag="VFB52_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06010.1"
                     /translation="KYDCIELVDTKQRRGATEHFYRATRRQFLTDSEWARMPESLRPG
                     LSGAMLKSVFDDIDEAVVGETFDELDDRHLSRTPMVVDEQGWKDVSELLAGALDRILV
                     IQAEANERLANSGENGMLSKVEILHFKSPPPKEDSDDSSE"
BASE COUNT          971 a         2212 c         2218 g         1000 t
ORIGIN      
        1 cgagctggtc gcgcagggca aggtcgccca cctcggcctc tccgaggcgg cgccggagac
       61 gatccgccgc gcccacgcga cccacccgat cgccgccctg cagaccgagt actcgctctg
      121 gagtcgcgag cccgaggacg agatcctccc gaccgtgcgt gagcttggga tcggatttgt
      181 tccctacagc ccgctcggcc gcggcttcct caccggtcgc ttccagagtc tcgacgacct
      241 gcccgagggc gacttccgcc gcgtcaaccc gcgcttcgaa ggcgagaact tcgatcgcaa
      301 cctacggctg gtcgagcggg tcacggagat cgccgcggag aagggggcaa cccccggcca
      361 gctggcgctc gcctgggtgc tcgcccaggg cgaggacgtc gtcccgatcc ccggcaccaa
      421 gcggctctcc tatctcgagc agaacgtcgc cgcggccgag atcgagctga gcgaggagga
      481 cctgcgccgg atcgacgagg ccgcccccgc cgacgcggcc gccggcgacc gctacgccga
      541 catgtcctcg gtcaacggct agcgccgttg gcgaccgggt gctcgccggt ctcggcccgc
      601 tcctggctgc gcagcgcctc gaggacctcc tcggccagct gggagccgtc ctcgggatgc
      661 agcggggtcg cgttgacgcg caggacgacg ctgtcgtggt cgagttcctc gagcgagacg
      721 ctggggcggt agcgggtcgg gacggtgatc tcgcgcagca ggcgccgctc gacccgctgc
      781 gggctggcgt cggtcgggaa gcgcgcccgc acgtcgactt tctcgggctc gcgcaggggg
      841 acgacggtga gctggaggag cacgttgttg gggaccagca gccgatccgc tccctggctg
      901 agggtggtgt agaagaggcc gagcgaggag accgtgccct cgagcgagcc ggcgagggcg
      961 ccgccgacca gccgcacccg ttcgccgacc cggaagggcc gcgtgctctg cagcacgatc
     1021 ccggcgaaga tgccgccgag actctgctgc gccgcgaggc cgagcacgac cgcggtgaag
     1081 gcaccgccga ccgccagggt gctggcggtg acgccggcaa tccgcagcgc caggatcacc
     1141 actgcgctca tcgccagcag ccggatcgcg aagccgagcg tccccgcggt cgccgggtcc
     1201 aggcgccggt tcatccgcgg cgccagccct ttcgagagcc agtgggtagc ggcggagccg
     1261 acgatcacca gcacgaccac ggtgccgatc cgaatccact cgccgtagcc gggggcgatg
     1321 tgggcgcgct ggtggtagac gaccagcgtg gccgcgatca cgaccagggc cgccagcagc
     1381 ccgccgagcg ccttcttcga gaagcgcggg gtcagctcgt cgccgagccc gagcgcctgc
     1441 caggtgtggc tgcgcgtctc gaacaggtcg cggggcattt tcgggcggcg cggagcgggc
     1501 atcaattccg gctacccgcc gagcggcggc ttactcgctg tgtgacgcct gtccggaggt
     1561 ggggtggaag ggctcagccg cgcagggcgg cgacgccctc gtcacggctg ccgtgcaccg
     1621 ggatcgactg gtcgagcccg ctgacgtcga gcacccgctt cacctggtcg ctcggcgagc
     1681 agatcgcgag tcctttgccg ccgttgccgc tgagctgctg ccaggcgcgc acgatcagcg
     1741 caatgccggt cgagtcgatg aactcgcact cgcccaagtc gatcaggacc ccggcgtcgc
     1801 caccgttgac ggcggcgtcc agtgggcctt ccagatcagg ggcggtgctg aggtcgagct
     1861 caccgcgaac ggcgataacc cgcaccccgc cgacctctgc cgaagacgtc tcgaagggga
     1921 ctggatgcac gtcgccgatg ctaccgcagc agacaggaga ggtcatcgtc tgcacacggt
     1981 ttgccgccgc ttggccaggg gtactcggtg gcccaggcag tggtgcaagc tatgctcggc
     2041 gccgcaaacc ggacctgccg cggcgccctg ggataggaaa tttgaacacg cagactgacg
     2101 atcgacccgg gcttcggctg tcccttccag cccgtgctga gaacgtcgct gtcgtacgcc
     2161 acgctctcgc cggtctcggc gagcgcctgg ggatggacga cacctcgctc gccgatctga
     2221 agacggttgt caccgaggcg gcgatgaacg tcgtcgtcca cgcctatccc gagaccgagg
     2281 agggactgct cgcggtcgag gcccacggcg agcacgacgg gctgaccgtg gtcgtgcgcg
     2341 acttcggcag cgggatcagg ccgcgtccgg atcccgaacg tccgagcctg cggatcggcc
     2401 tcagcctgat cgccgcgctt tcctccagct tcgagatcaa aggcgggatc gaccgcggga
     2461 ccgagatccg catgcatttg ccgctgcgcg ctgccagcgg cgatggccgc ccggagctga
     2521 cccagcagtg ggagacgccg gaggcgaccg aggtcagggt ggggccgccg gagctggtcg
     2581 gcccggtgct aagccgcgtg ctcagcgccc tcgcggcacg ccacgaaatc accatcgacc
     2641 gcctctccga cacgatgctg ctcagcgacg cgatctccgc cgctgccccg cagggcttca
     2701 ccgacggcca cgttgagctc agcatcgccg atcgacccga gggcgttgag ctgcgggtcg
     2761 gaccgatgac cgaaggcggc gccaaccgcc tccgcaaggg cctcgacctt ccccaggtcg
     2821 gatcgctgga gagcttggcc gacgacctcc gcgtcgagca aggcgaggaa ggcgagttcc
     2881 tcgtcgtcgc aatcgactcg ctcgcggcct agccggccgc ggcggcttac tcctcccggc
     2941 ccgcttcgcg gttctgttct gcaacctcgg cccggattcg ctccagggtg cggcgcagga
     3001 ttcgcgagac gtgcatctgg gagtggccga tcttttcggc gatctggctc tgggtcatgt
     3061 cctcgacgaa gcgcagctgt aggaccaggc gctcgcgctc gtcgagctgc gggaggactc
     3121 cctccagggc cagcttgtcc tcgatcagct cgtagccggc atcctcgtcg ccgacccact
     3181 cggaggccgg cgattcgtcc tcctcgccgc ccaccgggcg gtcaagcgag agcgggcggc
     3241 ggttgtggtt ggcctccagc acctcgagga cttcgctcgg gtcgacctcc atcttggcgg
     3301 cgatctccgg caccgagggc gagcgctgca gggtgacggt cagctcggtg atcgctttct
     3361 cgacttcggc catgcggtcg tgcaggccgc gcggcacccg cacggtccag acccggtcgc
     3421 ggaagtggcg tttgagttcg ccgaggatgg tcggcgaggc aaaggcggtg aagggcgtgc
     3481 cgcgcgaggg gtcgaagcga tcgatcgcgt tgaccaggcc gaggctcgcc acctgcagga
     3541 gatcgtcgaa ggactcgctg gcgccgcggt agcgcagcgc caggcttttc gcgaacggca
     3601 ggtagcgctt gaccagctct tcacgggtgg ccgggtcgcg ccgctcgctg aaccgtttcc
     3661 agagcgcctg ctcgctggcc aggccatcgg tcgcgtgctc gtcgattgag ggcgaggagg
     3721 tagctggggt gttttcgctg tttgctgcca tcggtttctt tgcgcgccgg gttcagtacc
     3781 ggcgctgccc agagggcttg tcgggaagcc gtgcagcgcc cgggggaccg ccacggccgc
     3841 ccgtgttcac accatacctt gagcgatact catttctaag ccccgtcaat aaccagaaag
     3901 gcgcttcctc aatcatcctt atccggacaa cttggctcgc gatttcgctt ttcctcgccg
     3961 tcctcttcct cgtcatcacc gcgagctcct cacccgccgc ttcccgctgg cagccgcttc
     4021 cgaccacggc ggcctggcag tggcagttgc agggcaagct cgacctcagc gtcgaggcct
     4081 ccgtctacga catcgacggc ttcgaatcga gccgcgccga cgtgcgcgcc ctgcagcgcc
     4141 gcggccgcaa agtcgtctgt tacctcgacg tcggcagctg ggagacctac cggcccgacg
     4201 ccgcccgctt cccgcgctcg gtgatcggcc gcaagtacga cggcttcccg gacgagcgct
     4261 ggctcgacgt cagccgcttc cagctcttcg ccgcgccgct gcgcgagcgg atcgcgatgt
     4321 gcgcgcgcaa gggcttcgac gcggtcgagc ccgacaacct cgccggctgg gaaccggaaa
     4381 acaagaccgg cttcgcgatc acccgcgccg accagctccg cttcaaccgc tggatcgccc
     4441 gccaggtcca ccgtcacggg atggcggtcg cgctcaagaa cgacgggcgc caggccaagc
     4501 agttggtcgg cgacttcgac ttcgcgatcg tcgaggagtg cttccagtac gacgaatgcg
     4561 gcttctacaa gcccttcgtc gaacgcggca aggcggtctt cgtcgccgag tacgagctcg
     4621 aaccgagcga gttctgcgcc gccgccgacg atctggagtt cagcgcgatc cgcaagtcct
     4681 acgacctctt cgcccgtccc tggcgcccgt gtggcagcgg ataatctcgg gtagaggcgt
     4741 ccgaccgaac taggaggcga gatgggattc gaccagtacc acgagccgcc ggaggagctt
     4801 tcggcggaga cccgaacctt cgcgcggatc tgcgcgtcgc tggaagagga gtcgcaggcg
     4861 atcggctggt accaacagcg aatctcggtc gaggccgacc ccgacgcgcg ggcgatcatg
     4921 gaagacgctc agggcgagga gttcaagcac ttcgcgatga acctcgagtt cctgctgcgg
     4981 cggacgccgc gctggcgcgc gatctgcgaa ggcgtgctgt tcaaggacgg cgacatcgtc
     5041 gagcacggcg aggccgccga ggccgcctcc gaggacggcg gagacggggg agaggacgag
     5101 gcgggtggcg acgacgcctc ccttggaatc ggcgggatga aaggagcggg gcaatgaacc
     5161 accttctgcg cgggctggcg cccgtttccg aggccggctg gcagctgctc gacgacgagg
     5221 cgcgggagcg cctgcagggt ccgttggcgg cgcgccggat ggtcgacttc tccgggccgc
     5281 acggctggga gcactcggcg atcaatctcg gccgcaccga gaaactgccg ccgcccaacg
     5341 gcaaaggcgt cacggcgctg cgccggcggg tgctgcccct ggccgagttg cgcgctggct
     5401 tcacgatctc gcgggccgag ctcgccgaca acgaccgcgg cgcggtcgac gccgacctcg
     5461 cggcgctcga cgaggcggcc cggcagattg ccgtggccga gaacgtcgcc gtctttcacg
     5521 gttggaaaaa agcggggatc accggcatca ccgaggcctc gcccttcaaa cccgagaagc
     5581 tcggcaaaac cgcggagacc tacccgcgct gtgttgcccg cgcggtcgag tcgatccgcc
     5641 aggctggtgt cgaggggccc tacggcctgg cgcttggccc cgatgagtac acgcgcgtga
     5701 tcgagactgc cgagcacggc ggctacccgc tgttcgacca cctggcgaag atcctcggcg
     5761 gcccgatcgt ctgggcgccg ggagtcaagg gagcggtggt cctcagcctg cgcggcggcg
     5821 acttcctctt cgagtctggg caggacctgg cgatcggtta cgccagccac gatgccgcaa
     5881 acgtcgagct ctacctcgag gagagcttct ccttcgtcgt cgccacgccg gaggcggcgg
     5941 cgccgctcag ttcctgagcg gcgccggccg tctactcgga tgagtcgtcg ctgtcctctt
     6001 tcgggggcgg cgacttgaag tgaaggatct ccactttcga gagcatcccg ttctcgccgc
     6061 tgttcgccag tcgttcgttg gcttcggcct ggatcacgag gatccggtcg agcgcaccgg
     6121 cgagtagctc ggagacgtcc ttccagccct gctcgtcgac gaccatcggc gtccggctca
     6181 ggtgccggtc gtcgagctcg tcaaacgtct ctccgacaac ggcctcgtcg atgtcgtcga
     6241 acaccgattt cagcatcgct ccggagaggc cggggcggag gctctcgggc atccgcgccc
     6301 actcgctgtc ggtcaggaac tggcgtcggg tcgcccgata gaaatgctcg gttgcgccgc
     6361 ggcgctgctt ggtgtcgacc agctcgatgc agtcgtattt c
//
LOCUS       DASRIG010000234         4557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2188663, whole genome shotgun sequence.
ACCESSION   DASRIG010000234 DASRIG010000000
VERSION     DASRIG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188663"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFB52_06465"
     CDS             complement(<1..466)
                     /locus_tag="VFB52_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06011.1"
                     /translation="MRGIRGYAAIALFAGLALLAPAAASAAPGSISGTVTAADGGAAL
                     AGISVCPYLLADEEFEEVCTETEGAGKYTLAGLEPGDYKVEFWDESLGHLGEYYDNQE
                     FWSKAEVLTVGDGAAVTGIDAALDKPAQVSGVVKAAGSGLPIEEVEVCIWGTE"
     gene            complement(453..989)
                     /locus_tag="VFB52_06470"
     CDS             complement(453..989)
                     /locus_tag="VFB52_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZO06012.1"
                     /translation="MDERLSPLLEDLDHVRGERGAELELVMFGDFQCPFCLGAQSVIR
                     RVRERLGDRLVFAFRHLPIPERHPLAPLAAQASEAAAAQGQFWEYHDALFQAQPRLSP
                     ETMLEVAKDLRLDVDRVEAELDSGVHEPRVARDLASAEASGATGTPSFFVNGTRLYGA
                     YDAGSLVDALENGPDEGD"
     gene            1011..2549
                     /locus_tag="VFB52_06475"
     CDS             1011..2549
                     /locus_tag="VFB52_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZO06013.1"
                     /translation="MQDIESILLLLLAAALLVRIAEFGKIPAPIVLVLGGLAIALVPG
                     LPEVELEPDTIFLIFLPPLVYAAGWRTAPGELRALMRPLGLLSVGLVFLTAAVVAVVA
                     HALVPELGWAEAAILGAVLAPTDAVSATSIFRRMNAPERVRLLVEGESMINDGTALVL
                     YRIAVGVATGTAFSLGDAALEFVGVSLGGIAVGLAIGFVSDRIIRKQTDGGLVVLLTV
                     LTAYAAYIGAEEAHVSGILAAVVAGVYAGYRSPQTLDADTRLGAVSFWGVLVFGLEIL
                     LFVLLGLQLPGVVDTLNEGSSQVSELLLPVAAIAAASILVRLLFVLAMRGDAGDTFGQ
                     RFAVGWSGMRGAVSLAAALAVPLSVEGRPQIIFLTFALILVTLVGQGLSLPFIVKRLK
                     LEEPRRWSDEEAVARMEAAQSALDRIDEMDAEDRADEKQLVRLRDLYRSRFRMCVAVL
                     AGEDPERAARAERISDYGELRRELIGIERDELIELRKGGRLKNQTMRQIERDLDLEEA
                     RIRS"
     gene            complement(2568..3575)
                     /locus_tag="VFB52_06480"
     CDS             complement(2568..3575)
                     /locus_tag="VFB52_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06014.1"
                     /translation="MSTAVGSRVGRWLLAVTVALFCLPAAASAAGIVNGDFETGNLEG
                     WTLSNSTFEGGQGTWLAYAGTVLPPPFESTEFEVPAPPQGKYAAITAQSGPGTRIIYQ
                     DFTVATGGAQALSLIAYYRSSAPMVTGPGLDATSENNQQYRIDVIKPSAPITSVDPGD
                     ILLNVFATETGDPATLAPETISANLTPFAGQTVRLRIAEVDNGGFFNAGVDAVTYAGG
                     PTPPAPLGEVWLGKPVAKNRKTGVSTIAVGLPAAGTLKVQDLRSVTGNGKPRIKPVTM
                     SISKSETVQVQLRPTADALKTLKAKGKVSLRAAFTLTLADGRTISLDRLLALRLAKPK
                     S"
     gene            complement(3647..4489)
                     /locus_tag="VFB52_06485"
     CDS             complement(3647..4489)
                     /locus_tag="VFB52_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO06015.1"
                     /translation="MIRHRRVRARGIEFHVAEAGEGDDVVLCLHGWPQHWYEWRHLMP
                     ALADRHRVIAIDQRGCGESEATRSGYEKEELASDVLAVMDALGLERVKLVGHDWGGWI
                     GFLLCLREPQRFERYLALNILPPWVNMRNLVGHAWRFYYQQLILAPGLGYQLHRRGDF
                     VRRVLVGASTDKSVWTEEDLAAFADNLTDPERARAAVQMYRTFNLREALPIARGRYAQ
                     ARLTVPTLLLFGEDDFVMRPSMLGGYQKHADEMRVELVPGCGHFIVDERPDLVSARAR
                     EFFA"
BASE COUNT          652 a         1588 c         1577 g          740 t
ORIGIN      
        1 attcggtgcc ccagatgcag acttcgactt cttcgatcgg caggccgctg ccggccgctt
       61 tcaccacacc ggagacctgg gccggtttgt cgagcgcggc gtcgatcccg gtaaccgcag
      121 cgccgtcgcc caccgtcagg acctccgctt tgctccagaa ttcctggttg tcgtagtact
      181 cgccgaggtg gccgaggctt tcgtcccaga attcgacctt gtagtcgccc ggttcgagcc
      241 cggcgagggt gtatttcccg gcgccttcgg tttccgtgca gacctcttcg aattcttcgt
      301 cggccagtag gtagggacag acgctgatcc ccgccagggc cgcgcccccg tcggcggcgg
      361 tcacggtgcc ggagatagat cccggcgcgg cgctggcggc agccggcgcg agcagcgcca
      421 ggccggcgaa cagcgcgatt gccgcgtacc ccctaatccc cctcatcagg gccattttct
      481 agcgcatcga ccagcgaacc ggcgtcgtat gcgccgtaca ggcgggttcc gttgacgaag
      541 aagctcggcg tgccggtcgc gccgctggcc tccgccgagg cgaggtcgcg ggcgacgcgc
      601 ggctcgtgca cgccggagtc gagctcggcc tcgacccggt cgacgtcgag ccgcaggtcc
      661 ttggccactt ccagcatcgt ctccggcgag agccgcggct gggcctggaa gagcgcgtcg
      721 tggtactccc agaactggcc ctgggccgcc gccgcctcgc tcgcctgcgc ggccagcggc
      781 gccagggggt gacgctcggg gatcggcagg tggcggaagg cgaagacgag ccgatcgccg
      841 agccgctcac gcacccgccg gatcaccgac tgggcgccga ggcaaaacgg gcactggaag
      901 tcgccgaaca tcaccaactc cagctccgcg ccccgctcac cgcgcacgtg atcgaggtct
      961 tcgagtaggg gtgaaagtcg ttcgtccacg gaacgcgatg ttatgtggtg gtgcaagaca
     1021 tcgagtcgat cctcctcctg ctcctcgccg ctgccctgct ggtgcggatc gccgagttcg
     1081 ggaagatccc ggcgccgatc gtgctggtgc tcggcgggct ggcgatagcg ctggtcccgg
     1141 gcctgcccga ggtcgaactc gagccggaca cgatcttcct gatcttcctg ccgccgctcg
     1201 tctacgctgc cggctggcga accgcgccgg gcgagctgcg ggcgctgatg cggccgctgg
     1261 ggctgctttc ggtcgggctc gtcttcctca ccgccgcggt cgtcgccgtc gtcgcccacg
     1321 cgctggtgcc ggagctgggc tgggcggaag cggcgatcct cggcgccgtg ctcgccccca
     1381 ccgacgcggt ctcggcgacc tcgatcttcc gccgcatgaa cgcccccgag cgggtgcggc
     1441 tgctggtcga gggcgagtcg atgatcaacg acggcaccgc cctggtcctc taccggatcg
     1501 cggtcggggt cgcgaccggc accgccttca gcctcggcga cgcggcgctc gagttcgtcg
     1561 gcgtctcgct cggcggcatc gctgtcgggc tcgcgatcgg gttcgtctcc gaccgcatca
     1621 tccgcaagca gaccgacggc ggcctggtcg tcctgctgac cgtgctgacc gcctacgcgg
     1681 cctacatcgg cgccgaggag gcgcacgtct ccggcatcct cgccgccgtc gtcgccgggg
     1741 tctacgccgg ctaccgctcg ccgcaaacgc tcgacgccga cacccggctc ggcgccgtct
     1801 ccttctgggg cgtgctcgtc ttcgggctcg agatcctgct cttcgtgctg ctcgggctgc
     1861 agctcccggg ggtcgtcgac acgctcaacg aaggctcctc ccaggtcagc gagctgctgt
     1921 tgccggtggc ggcgatcgcc gctgcctcga tcctcgtccg cctgctcttc gtgctggcga
     1981 tgcgcggcga cgccggcgac accttcggcc agcgcttcgc ggtcggctgg agcgggatgc
     2041 gcggcgccgt ctccctggcc gccgccctgg cggtgccgct cagcgtcgag ggccgcccgc
     2101 agatcatctt cctcaccttc gccctgatcc tcgtcaccct ggtcggccag gggctctcgc
     2161 tgccgttcat cgtcaagcgg ctgaagctgg aggagccacg gcgctggagc gacgaagagg
     2221 cggtggcgcg gatggaggcg gcgcagtcgg cgctcgaccg gatcgacgaa atggatgccg
     2281 aggaccgcgc cgacgagaaa cagctggtgc ggctgcgcga cctctaccgc tcgcgcttcc
     2341 gcatgtgcgt ggcggtgctg gccggtgagg accccgagcg cgccgcccgc gccgagcgca
     2401 tctccgacta cggcgagctg cgccgcgagc tgatcgggat cgagcgcgac gagctgatcg
     2461 agctgcgcaa aggcggccgg ctgaaaaacc agacgatgcg ccagatcgaa cgcgacctcg
     2521 acctcgagga ggcgcggatc aggtcgtgat ccgcgcctcc ctccggccta cgacttcggc
     2581 ttcgccaggc gcagcgccag caggcggtcg agcgagatcg tgcgaccgtc ggcgagcgtc
     2641 agcgtgaagg cggcgcgcag cgagactttg cccttcgcct tcagcgtctt cagcgcgtcg
     2701 gcggtcgggc gcaactggac ctggacggtc tccgacttcg agatcgacat ggtcaccggc
     2761 ttgatccgcg gcttgccgtt gccggtcacc gagcgcagat cctgcacttt cagcgtaccc
     2821 gcggcgggca ggccgacagc gatcgtcgag acgccggtct tgcggttctt cgcgaccggc
     2881 ttgccgagcc agacttcgcc gagcggggcc ggcggggtcg gacccccggc gtaggtgacc
     2941 gcgtcgacgc cggcgttgaa gaagcccccg ttgtcgacct cggcgatgcg caggcgcacg
     3001 gtctgaccgg cgaagggagt gaggttggcg gagatcgttt ccggtgccag ggtcgccgga
     3061 tcgccggtct ccgtcgcgaa cacgttgagc aggatgtcac cggggtcgac cgaggtgatc
     3121 ggagcactcg gtttgatcac gtcgatccgg tattgctggt tgttttcgct ggtcgcgtcg
     3181 aggcccgggc cggtgaccat cggggccgag ctcctgtagt aggcgatcag cgagagcgcc
     3241 tgagcgccgc cggtagcgac cgtgaagtcc tggtagatga tccgggtccc ggggccgctc
     3301 tgcgcggtga tcgccgcgta tttgccctgc gggggagccg ggacttcgaa ttccgtcgat
     3361 tcgaagggag gcgggaggac cgtgccggcg taggcgagcc aggtgccttg gccgccttcg
     3421 aaggtcgagt tcgaaagcgt ccaaccttcc aggttgccgg tctcgaagtc tccgttgacg
     3481 atcccggcgg cgctggccgc ggccggaagg cagaacaagg cgacggtgac ggcgagcagc
     3541 cagcgaccga cgcgcgatcc aacagcagta gacatcagga ttcccctttg cagcgaacgt
     3601 tttacgaacg gatagacaac ccgcatcggc ggacgaaccg gttcgcttaa gcgaagaact
     3661 cgcgcgcccg cgcggaaacc aggtcgggac gctcgtcgac gatgaagtga ccgcaccctg
     3721 gaaccagctc gacccgcatc tcgtcggcgt gcttctggta gccgccgagc atcgaggggc
     3781 gcatcacgaa gtcgtcttcg ccgaacagca gcagggtcgg cacggtcagg cgcgcctgcg
     3841 cgtagcggcc gcgggcgatc ggcagcgcct cgcgcaggtt gaacgtcctg tacatctgca
     3901 ccgccgcccg cgctcgctcc ggatcggtga ggttatccgc gaaggcggcg agatcctcct
     3961 cggtccagac gctcttgtcc gtcgaggctc caaccaggac gcggcgcacg aagtcgccgc
     4021 ggcggtgcag ctggtagccg agccccggcg ccaggatcag ctgctggtag tagaaccgcc
     4081 aggcgtggcc gaccaggttc cgcatgttga cccagggcgg caggatgttc agcgccaggt
     4141 agcgctcgaa gcgctgcggc tcgcgcaggc agagcaggaa cccgatccag ccgccccagt
     4201 catggccgac cagcttcacc cgctcgagcc cgagcgcgtc catcaccgcg agcacgtcgc
     4261 tggccagctc ctccttctcg taacccgagc gggtcgcctc ggattcgccg cagccgcgct
     4321 ggtcgatcgc gatcacccgg tggcggtcgg ccagcgccgg catcaggtga cgccactcgt
     4381 accagtgctg cggccagccg tgcaggcaga ggacgacgtc gtcgccctcc ccggcttcag
     4441 cgacatggaa ctcgattccg cgcgcgcgga cccgccggtg gcggatcaac gagcgttgct
     4501 tgtgacgccg ccctcgcgga actcgacgac aacttcctcc atcgtctccg cgttgca
//
LOCUS       DASRIG010000235         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_644293, whole genome shotgun sequence.
ACCESSION   DASRIG010000235 DASRIG010000000
VERSION     DASRIG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644293"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFB52_06490"
     CDS             <1..609
                     /locus_tag="VFB52_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06016.1"
                     /translation="GVNSIITTLGTASVLSGLAIWISKGNTLATGISPELLEFGSGKT
                     AGIPHVFFVLIAVVIVAGYLLRQTPFGRSLYALGDNERAARLVGMPVGRMTVVAFVIG
                     GALAGCGGVLQVAAAGSADPQFGPNLLLPALSAVFLGATAIRPGEYNVTGTLIAVAFN
                     AVSISGLLLAGVADWVQPVYIGVTLVVAVALSTVLRKRRTGQ"
     gene            606..1349
                     /locus_tag="VFB52_06495"
     CDS             606..1349
                     /locus_tag="VFB52_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06017.1"
                     /translation="MIAAGKVALVAGGASGLGEAAAQALSEAGATVVVLDLNEPVEAV
                     DGIEYRTGDVRDPEQVGAAVDYAAGLGELRIAACCAGVAAAGRITRKGAAHDLAEFQR
                     VIDINLVGTFNVLRLAATAMTALEPVEEERGVLISTASIAAYDGQMGQVAYAASKGGI
                     VGMTLPVARDLADSAVRCVAIAPGLFETPLLGQLPQEAREALAKTIPHPSRLGRPAEF
                     GRLVVDVVGNPMLNGETLRLDGALRMAPR"
     gene            1349..2941
                     /locus_tag="VFB52_06500"
     CDS             1349..2941
                     /locus_tag="VFB52_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06018.1"
                     /translation="MLAPQTNLTTILSRQAAVPGGRDRPAVSCAGEERSFAELDERAR
                     RVAAGLVAAGVRPGDRIAVLMQNRIEWVELLFGIAAAGAVCVPISILLRAGEIAYICE
                     DSGAAWVVADERSLPLLEELPAGVETVVVVGEGEPPVARSTFGFDQLLAADPHAGPGP
                     GRDDLAIIYYSSGTTGKPKGAAHSHAGVLWNAIGQVVDIGLTADHTYLLVPSLSWAAG
                     FNNLFHSVLWVGGRSVILPSTETSIEDTVANLETSAATHSMLVPTLVRQLLDRPDLVE
                     RVRGTELRWVLCGGEPIPRSLIDKWDEGLPGVQLAQAYGMSEFPTIATILRPEHASSH
                     AGTAGRAMSHTEIAVRTDDGRIERTGEGEVLLRSLAVMRGYHGNPEETERVFADGWFA
                     SGDRGLIDADGFLSITGRVKDMIISGGLNVYPREIEEVIYRLDEVTEACVVGVPDEQW
                     GEVPAAVVVSEQEGWDGAAVIPHCREHLASFKCPKHVIVRAEPLPRNPSGKVLKREVA
                     PWAQSQLETSTQPKPAEVPNAH"
     gene            2931..>3560
                     /locus_tag="VFB52_06505"
     CDS             2931..>3560
                     /locus_tag="VFB52_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06019.1"
                     /translation="MPIDFSVEPEFQEKLDWIAELLKDEIYPLEVLGLNHAQLLEMVA
                     PLRERVVERQLWAPHLDRELGGQGFGQVKMGLIHELLGRSRIAPFVFGCQAPDSGNSE
                     ILALNANDEQKEKWLYPLLDGEIYSSYAMTEPGAGADPTLLSAEAVLDGDEWVINGDK
                     WFITNASIADFFIIMLVTEPEAAPHARASQIIVPVDTPGVNVAREIGTIE"
BASE COUNT          504 a         1273 c         1255 g          528 t
ORIGIN      
        1 ggcgtcaact cgatcatcac cacgctcggc accgcctcgg tgctctccgg tctggcgatc
       61 tggatcagca agggcaacac gctggccacc gggatctccc cagagctgct cgaattcggc
      121 tccggaaaaa cggccgggat cccgcacgtc ttcttcgtcc tgatcgcggt cgtgatcgtc
      181 gccggctacc tgctcaggca gaccccgttc gggcgctcgc tctacgcgct gggcgacaac
      241 gagcgggcgg cgcgcctggt cgggatgccg gtcggccgga tgaccgtcgt cgccttcgtg
      301 atcggcggcg ccctggccgg ctgcggcggc gtccttcagg tcgccgcggc gggctcggcc
      361 gacccccagt tcgggcccaa cctgctgctg ccggcgctga gcgccgtctt cctcggcgcc
      421 accgcgatcc gtcccggcga gtacaacgtc accggcacgc tgatcgcggt cgccttcaac
      481 gcggtctcga tcagcggcct gctgctggcc ggcgtcgccg actgggtcca gccggtctac
      541 atcggcgtca ccctggtggt cgcggtcgcc ctctccaccg tgctgcgcaa gcggaggacc
      601 ggccagtgat cgccgcaggc aaggtcgcgc tggtcgccgg cggggcctcc gggctcggcg
      661 aagcggcggc ccaggcgctg agcgaagccg gggcgacggt tgtcgtgctc gacctcaacg
      721 aaccggtcga ggccgtcgac gggatcgagt accgcacggg cgacgtgcgc gaccccgagc
      781 aggtcggcgc ggcggtcgac tacgcggccg ggctcggtga gctgcggatc gccgcctgct
      841 gtgcgggcgt ggccgccgcc gggcggatca cccgcaaggg cgccgctcac gacctcgccg
      901 agttccagcg ggtgatcgac atcaacctgg tcggcacctt caacgtgctg cgcctcgcgg
      961 cgaccgcgat gaccgcgctg gagccggtcg aagaagagcg cggggtgctg atcagcaccg
     1021 cctcgatcgc cgcctacgat ggccagatgg gccaggttgc ctacgccgcc tcgaagggcg
     1081 gcatcgtcgg tatgaccctg ccggtcgccc gcgacctcgc ggactcggcg gtccgctgcg
     1141 tggcgatcgc ccctggcctg ttcgagaccc cgctgctggg acagctcccg caggaggcgc
     1201 gcgaggcgct ggcgaagacg attccccacc cctcgcggct cgggcggccc gctgagttcg
     1261 gccggctcgt cgtcgacgtg gtcgggaacc cgatgctgaa cggagagacg ctccgcctcg
     1321 acggggcgct gcggatggcg ccgcgctgat gctcgcgccg cagacgaacc tgaccacgat
     1381 cctgtcccgg caggccgcgg tgccgggcgg gcgcgaccgg cccgccgtct cctgcgccgg
     1441 tgaggagcgc agcttcgccg agctcgacga gcgggcccgt cgggtcgccg cagggctggt
     1501 cgcggccggg gtccggcccg gcgaccggat cgcggtgttg atgcagaacc ggatcgagtg
     1561 ggtcgagctg ctgttcggga tcgccgccgc cggcgcggtc tgcgtgccga tcagcatcct
     1621 cctgcgggcc ggcgagatcg cctacatctg cgaagactcc ggcgccgcct gggtggtcgc
     1681 cgacgagcgc agcctgccgc tgctcgagga gctgcccgcg ggcgtggaga cggtcgtcgt
     1741 ggtcggcgag ggcgagccgc cggtggcgcg ctcgacgttt ggtttcgacc agctgctcgc
     1801 agccgatccc cacgccggcc cgggtcccgg ccgcgacgac ctcgcgatca tctactactc
     1861 ctcgggaacc accggcaagc cgaagggcgc cgcccacagt cacgccgggg tgctctggaa
     1921 cgcgatcggc caggtggtcg acatcggcct taccgccgac cacacctacc ttctggtccc
     1981 ctcgctctcc tgggccgcgg gtttcaacaa cctcttccac tcggtgctct gggtcggcgg
     2041 ccgctcggtg atcctgccca gcaccgaaac ctcgatcgag gacacggtcg ccaacctcga
     2101 gacctcggcg gcgacgcact cgatgctggt cccgaccttg gtccgccagc tgctcgaccg
     2161 ccccgacctg gtcgagcggg tgcgcggcac cgagctgcgc tgggtgctct gcggcggcga
     2221 gccgatcccg cgcagcctga tcgacaagtg ggacgaggga ctgcccggcg tccagctcgc
     2281 ccaggcctac gggatgtcgg agttcccgac gatcgcgacg atcctgcggc ccgagcacgc
     2341 gagctcccac gccggcaccg ccgggcgggc gatgagccac accgagatcg cggtgcgcac
     2401 cgacgacggc cggatcgagc ggaccggcga gggcgaggtg ctgctgcgct cgctggcggt
     2461 gatgcgcggc taccacggca acccggagga gaccgaacgg gtcttcgccg acggctggtt
     2521 cgccagcggc gaccgcggcc tgatcgacgc ggatgggttc ctctcgatca ccggccgggt
     2581 caaggacatg atcatctccg gcgggctcaa cgtctacccg cgcgagatcg aggaggtgat
     2641 ctaccggctc gacgaggtca ccgaggcctg cgtggtcggc gtacccgacg agcagtgggg
     2701 cgaggtcccg gcggcggtgg tcgtctccga gcaggagggc tgggacggcg ccgcggtgat
     2761 cccccactgt cgtgagcatc tcgcctcctt caaatgcccc aagcacgtga tcgtccgcgc
     2821 cgagccgttg ccgcggaacc cgtcggggaa ggtgctcaag cgcgaggtcg cgccctgggc
     2881 ccagagccag ctcgaaacgt ccacgcaacc gaaaccagcg gaggttccca atgcccattg
     2941 acttctcggt cgagccggag ttccaggaga agctcgactg gatcgccgag ctgctgaagg
     3001 atgagatcta tcccctcgag gtgctcggcc tcaatcacgc gcagctgctg gagatggtcg
     3061 cgccgctgcg cgagcgggtg gtcgagcggc agctctgggc gccgcacctc gaccgcgagc
     3121 tcggcggcca gggtttcggc caggtgaaga tgggcctgat ccacgaactg ctcggtcgct
     3181 cgcgcatcgc cccctttgta ttcggctgcc aggccccgga ctcgggcaat agcgagatcc
     3241 tcgccctcaa cgccaacgac gagcagaagg agaaatggct ctacccgttg ctcgacggcg
     3301 agatctactc ctcctacgcg atgaccgagc cgggggctgg cgccgacccg accctgctct
     3361 cggccgaggc ggtgctcgac ggcgacgagt gggtgatcaa cggcgacaag tggtttatca
     3421 ccaacgcctc gatcgccgac ttcttcatca tcatgctggt caccgagccc gaggcggcgc
     3481 cgcacgcccg cgcctcgcag atcatcgtcc cggtcgacac cccgggggtg aacgtggccc
     3541 gggagatcgg cacgatcgag
//
LOCUS       DASRIG010000236         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_645879, whole genome shotgun sequence.
ACCESSION   DASRIG010000236 DASRIG010000000
VERSION     DASRIG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645879"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFB52_06510"
     CDS             complement(<1..186)
                     /locus_tag="VFB52_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO06020.1"
                     /translation="MSATAEITGRYVSIDGTRTFYDECGEGPPVVCVHTAGADSREYQ
                     YLLPELAARGYRGLALDL"
     gene            complement(183..641)
                     /locus_tag="VFB52_06515"
     CDS             complement(183..641)
                     /locus_tag="VFB52_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06021.1"
                     /translation="MKIQDPEFRERLIAALADEPEFEQEARWFDGSILLQVGAEPLWL
                     KVYRGKVIDTLEFTPVFGYTFKVSGSPQAWAMLGSGERTFTDLASAGSRYLGSIEEIE
                     AAGGGYKPPELAIEGNGVEAGRMHLATMYLCRAFAATAVESGAVEAGVAA"
     gene            complement(705..1829)
                     /locus_tag="VFB52_06520"
     CDS             complement(705..1829)
                     /locus_tag="VFB52_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO06022.1"
                     /translation="MTKAAASRAVVQTGVGSFEVHEFGIPEIGPDEGLLRMELCGICG
                     SDVEQYDGRFDELGWTVGPTIPGHEPLGIIERVGERAAKRWGVEPGDRVAVEPLIPCG
                     ECEACREGQRTRCSGWGRMYSYGILGTDIEPQILGGYSEYMYLHPNTVMHKMPREMPA
                     SVAVLFNPLAAGVRWATTTPDLSLGETIVVLGAGQRGLSCVIAAKAAGAGKIIVTDLA
                     RAAAKLEFARELGADATIVADEEDVVARVMDLTGGRGADVAVDVTPVATQPLIDAVDF
                     VRNGGTIVLSGVKGGPKLELDPDKVMHKSITLRGVFTVDSPAYRRAIALLEGGGRPFE
                     RIHTASYPLERAEEAIHHLAGRLDGPPAVHVAIAPGAAPS"
     gene            complement(1852..>2537)
                     /locus_tag="VFB52_06525"
     CDS             complement(1852..>2537)
                     /locus_tag="VFB52_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06023.1"
                     /translation="IGLAERVEFLILFASTDRSKGARGVTAFLIDSGTPGYNIVRLIQ
                     TMGDAWVPTEIEFDDCRIPATQRLGEEGEAFALADEQLSHGRLKIASYQLGIAQRCLD
                     EAVAYAQERTTWGQPIASRQGIQFPLADSAVELDVARLLVYKTAWRADRGEDVRIDAF
                     KSKLYCTEMAQRVTDRCLQVFGGRGYAKENPIQSYFRQARLWRIGHGTAEIHRWMIAR
                     DLLGPAAAA"
BASE COUNT          339 a          898 c          908 g          392 t
ORIGIN      
        1 caggtcgagg gcgagcccgc ggtatccgcg cgccgccagc tccggcagca ggtactggta
       61 ctcgcgcgag tcggcgccgg cggtgtggac gcagacgacc ggggggccct cgccgcactc
      121 gtcgtagaag gtccgggtgc cgtcgatgct gacgtagcgg ccggtgatct cagcggtggc
      181 gctcatgcgg ccacccccgc ctcgaccgcg ccgctctcca cggcggtcgc cgcgaaggcg
      241 cggcagaggt acatggtcgc gaggtgcatg cggccggctt cgaccccgtt gccctcgatc
      301 gccagctccg gcggcttgta gccgccaccg gccgcctcga tctcctcgat cgagccgagg
      361 tagcgcgagc cggccgaggc cagatcggtg aaggtgcgct cgccgctgcc gagcatcgcc
      421 cacgcctgcg gcgagccaga gaccttgaac gtgtaaccga agaccggggt gaattcgagc
      481 gtgtcgatca ccttgccgcg atagaccttc agccagagcg gttcggcgcc gacctgcagc
      541 aggatcgagc cgtcgaacca gcgcgcctcc tgctcgaact cgggttcgtc cgcgagcgct
      601 gcgatcaagc gctcccggaa ctcggggtcc tggattttca ctgctctcct ccttctcgtt
      661 tcccaaatcg gtgctgcgaa agcggtgctg cgaactgctg cgggtcagga cggcgccgcc
      721 cccggcgcga tcgcgacgtg cactgccggc ggaccgtcga gccggccggc caggtggtgg
      781 atcgcctcct cggcgcgctc cagcgggtag ctggcggtgt ggatccgctc gaacgggcgc
      841 ccgccgcctt cgagcagcgc gatcgcccgc cggtaggcgg gtgagtcgac ggtgaagacc
      901 ccgcgcaggg tgatcgactt gtgcatcacc ttgtcggggt cgagctccag tttcggcccg
      961 cccttgaccc cgctgagcac gatcgtgccg ccgttgcgga cgaagtcgac cgcgtcgatc
     1021 agcggctgcg tcgccaccgg ggtgacgtcg acggcgacgt cggcgccgcg ccccccggtc
     1081 aggtccatga cccgggcgac cacgtcctcc tcgtcggcga cgatcgtcgc gtcggcgccg
     1141 agctcccgcg cgaactcgag tttggccgcg gcccgggcga ggtcggtgac gatgatcttg
     1201 ccggccccgg cggccttggc ggcgatcacg caggagagcc cgcgctggcc ggccccgagc
     1261 acgacgatcg tctcgccgag cgagaggtcg ggcgtcgtcg tcgcccagcg caccccggcg
     1321 gccagcgggt tgaagaggac cgcgaccgag gccggcatct cgcgcggcat cttgtgcatc
     1381 accgtgttcg ggtgcaggta catgtactcg gagtagccgc cgaggatctg gggctcgatg
     1441 tcggtgccga ggatcccgta gctgtacatc cgaccccagc cgctgcatcg cgtgcgctga
     1501 ccttcgcggc aggcctcgca ctcgccgcag gggatcagcg gctcgaccgc gacccggtcg
     1561 ccgggctcga cgccccagcg cttcgccgcc cgctcgccga cccgctcgat gatcccgagc
     1621 ggctcgtggc cgggaatcgt cgggccgacc gtccagccga gctcgtcgaa gcggccgtcg
     1681 tactgctcga cgtcggagcc gcagatcccg cacagctcca tccgcaggag cccctcgtcg
     1741 gggccgatct cggggatccc gaactcgtgg acctcgaagc tgccgacgcc ggtctggacg
     1801 accgcccggc tcgctgctgc cttggtcatc cgttcctctc cgcggccgcg cctacgcggc
     1861 cgccgccggt ccgagcaggt cgcgggcgat catccagcgg tggatctcgg cggtgccgtg
     1921 gccgatccgc cagagccgcg cttggcggaa gtagctctgg atcgggttct ctttcgcgta
     1981 gccgcggccg ccgaagacct gcaggcagcg gtcggtgacc cgctgcgcca tctcggtgca
     2041 gtagagcttc gacttgaagg cgtcgatgcg cacgtcttcg ccgcggtcgg cgcgccaggc
     2101 ggtcttgtag acgagcaggc gggcgacgtc gagctcgacc gccgagtcgg cgagcgggaa
     2161 ctggatcccc tgacggctgg cgatcggctg gccccaggtg gtgcgctcct gcgcgtaggc
     2221 gaccgcctcg tcgaggcagc gctgggcgat cccgagctgg tagctcgcga tcttcaggcg
     2281 gccgtggctg agctgctcgt cggcgagcgc gaacgcctcg ccctcctcgc cgagccgctg
     2341 ggtcgccggg atccggcagt cgtcgaactc gatttcggtc ggcacccagg cgtcgcccat
     2401 ggtctggatc aagcgcacga tgttgtagcc gggggtgccg gagtcgatca ggaatgcagt
     2461 gacgccgcgg gcccccttgg agcggtcggt ggaggcgaag aggatcagga actcgacccg
     2521 ctcggcgagg ccgatga
//
LOCUS       DASRIG010000237         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3784853, whole genome shotgun sequence.
ACCESSION   DASRIG010000237 DASRIG010000000
VERSION     DASRIG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784853"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFB52_06530"
     CDS             <1..830
                     /locus_tag="VFB52_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06024.1"
                     /translation="RHDNFLASNLSQFLAGMVELGLGFLTPFFLLLVVGVSPATAGIC
                     LIPATIPIILAGPLSGRAFDRFGGRWPLVVGFLCLAASGLVLAVSVSEQTAVALIPGL
                     LLQGLGLGIVLTVNDPTGLTAVPPEDSGEAAGMINTSEQLGGAIGIAALTAVELGINF
                     AKIEDRLAERGIHPTPEQNEHARELIVQGEQAGLKNLPHSHLLEIVQPVVADAHVTAF
                     QVTFAASAGIALTGALVCLLLVRKTDRVASGPIFSRRSRWVYVSHGRSSAISKRPPP"
     gene            complement(845..2746)
                     /locus_tag="VFB52_06535"
     CDS             complement(845..2746)
                     /locus_tag="VFB52_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06025.1"
                     /translation="MQIPAAERLRPWLTTVSRSPRAAALLGPVVALLVWRVAMSPAGV
                     GLDSSWNAGLTMATKHGLQFGQEVVFSYGPLGFLQSPYIFYSDLGLIAFLFTATVYVG
                     FCIALVWGLGRVLPPLPSAAVAFALLAVTTVQEQALLIAMLAALTLLERPPSQRALWV
                     FALAGASFAAVEGLIKLSTGPLLAAVFLLALIGARARREQVAAYVVVLAAELALLWAL
                     TGQSFGAIPDFATNTWEIASGYSAAMLREVDVAPWKVTFATISAALITVALVVACARA
                     EFADRRARWAGALAMALVGFMVFKEGVVRTDAGHLNLYFATATLLWLAIPWARSRWPL
                     ALAGAAAIIAIGIPVHPSVSSANFDVVTSLRSAGDQVRNVFSSGRRDDLVETGREATA
                     AVYALEPEALAALRGRTVAVEPWEAAAAWSYELDWSPLPVYQNYSAYTPELDRLNAAA
                     AESPDGPERILRENPLLVFPEFPTAGLDNRYPGWDPPEQQRAVLCNFIPVQSSERWQV
                     LARIPDRCGEPRPLGTVEAAAGDTVPVPQPRPGEFVFVRIEGAGVGGLERLTTMLLHA
                     ETRRLVLDDGRSYRLLPETAGDGLLMAGRGAFVEAGPFATIPQARSIALTGIDAELEY
                     AFFAVRVRG"
BASE COUNT          402 a         1062 c          990 g          372 t
ORIGIN      
        1 tccgccacga caacttcctc gcctccaacc tcagccagtt cctcgccggg atggtcgagc
       61 tcgggctcgg cttcctcacc ccgttcttcc tcctgctcgt ggtcggcgtc tcgccggcga
      121 ccgccggcat ctgcctgatc ccggcgacga tcccgatcat cctcgccggc ccgctctcgg
      181 gccgcgcctt cgaccgcttc ggcggccgct ggccgctggt cgtcggcttc ctctgcctcg
      241 ctgcctccgg gctggtgctc gccgtctcgg tctccgagca gaccgcggtg gcgctgatcc
      301 ccggcctgct tctgcagggc ctcggcctcg gcatcgtcct caccgtcaac gacccgaccg
      361 ggctgaccgc ggtcccgccc gaggacagcg gcgaggcagc gggcatgatc aacacctccg
      421 agcagctcgg cggcgcgatc gggatcgcgg cgctgactgc ggtggagctc gggatcaact
      481 tcgccaagat cgaagaccgg ctggccgaac gcggcatcca cccgaccccc gaacagaacg
      541 aacacgcccg cgaactgatc gtccagggcg agcaggccgg gctgaaaaac ctgccccaca
      601 gccacctgct ggaaatcgtc cagccggtcg tcgccgacgc gcacgtgacc gccttccagg
      661 tcaccttcgc cgcctccgcc gggatcgccc tgaccggcgc gctcgtctgc ctcctgctcg
      721 tgcgcaagac ggaccgggtc gcctccggcc cgatcttcag ccgccgctcc cgctgggtct
      781 acgtctccca cggccgctcc tcggcgatca gcaaacgccc cccgccgtag cggccgggcc
      841 cggctcagcc gcgcacgcgg acggcgaaga acgcgtactc gagctcggcg tcgatcccgg
      901 tcagcgcgat cgagcgcgcc tgcgggatcg tcgcgaacgg cccggcctcg acgaaggcgc
      961 cgcggccggc catcagcagc ccgtcgcccg cggtttccgg cagcagccgg tagctgcggc
     1021 cgtcgtcgag cacgagccgg cgggtctcgg cgtgcagcag catggtggta agtcgctcga
     1081 gcccgccgac gccggccccc tcgatgcgga cgaagacgaa ctcgccgggc cgcggctgcg
     1141 gtaccggaac tgtgtcgccc gccgccgcct cgaccgttcc gagtggccgc ggctccccgc
     1201 agcggtccgg gatccgggcg agcacctgcc agcgttcgct gctctggacc gggatgaagt
     1261 tgcagaggac cgcgcgctgc tgttcgggtg ggtcccagcc gggatagcgg ttgtcgagcc
     1321 ccgcggtcgg gaactcgggg aagacgagca gcgggttctc gcgcaggatc cgctcgggcc
     1381 cgtccgggct ctcggcggcg gccgcgttca gccggtcgag ctcgggcgtg taggcggagt
     1441 agttctggta gaccggcagc ggcgaccagt ccagctcgta gctccaggcg gccgccgcct
     1501 cccacggttc gaccgcgacc gtgcgcccgc gcagcgccgc cagcgcctcc ggctcgagcg
     1561 cgtagaccgc cgcagtcgct tcccgtccgg tctcgaccag gtcatcgcgc cgcccgctgc
     1621 tgaacacgtt gcgcacctgg tccccggcgc tgcgcaggct ggtgacgacg tcgaagttgg
     1681 cgctgctgac cgacgggtgg accgggatgc cgatcgcgat gatcgccgcc gcgccggcca
     1741 gcgccagcgg ccagcgtgag cgcgcccagg ggatcgccag ccagagcagg gtggcggtcg
     1801 cgaagtagag gttgaggtgg ccggcgtcgg tcctgaccac gccctccttg aagaccatga
     1861 acccgaccag cgccatcgcc agcgccccgg cccagcgggc ccggcggtcg gcgaactcgg
     1921 cgcgcgcgca ggcgacgacc agggcgaccg tgatcagcgc cgcgctgatc gtcgcgaagg
     1981 tcaccttcca gggggcgacg tcgacttcgc gcagcatcgc ggcgctgtag ccggaggcga
     2041 tttcccaggt gttggtggcg aagtcgggga tcgcgccgaa gctctgcccg gttagcgccc
     2101 agagcagcgc cagctcggcg gcgagcacga cgacgtaggc ggcgacctgc tcgcgccgcg
     2161 cccgggcgcc gatcagggcg agcaggaaga ccgcggccag cagcggcccg gtggagagct
     2221 tgatcagccc ctcgaccgcg gcgaagctcg cgcccgccag cgcgaacacc cacagcgccc
     2281 gctgcgaggg cgggcgctcg agcagggtca gtgcggccag catcgcgatc agcagcgcct
     2341 gctcctgcac ggtcgtgact gcgagcaggg cgaaggcgac ggcggcgctc ggcagcggcg
     2401 gcagcacccg tcccagaccc cagaccaggg cgatgcagaa gccgacgtag acggtggcgg
     2461 tgaagaggaa ggcgatcagc ccgaggtccg agtagaagat gtaggggctc tgcaggaagc
     2521 cgagcggccc gtaggagaag acgacctcct ggccgaactg caggccgtgc ttggtcgcca
     2581 tcgtcaggcc ggcgttccag ctcgagtcga gccccacccc ggccgggctc atcgcgaccc
     2641 tccagaccag cagggcgacc accggcccga gcagtgccgc ggcccgcggc gagcgcgaga
     2701 ccgtggtcag ccacgggcgc aggcgctcag ctgccgggat ctgcatctcg cccgcgctcc
     2761 aacgccgtga tcgccaggct gagcccgagt ttggccccga tcccggcgta gaccgcggcg
     2821 ttcagc
//
LOCUS       DASRIG010000238         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1681116, whole genome shotgun sequence.
ACCESSION   DASRIG010000238 DASRIG010000000
VERSION     DASRIG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681116"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFB52_06540"
     CDS             <1..535
                     /locus_tag="VFB52_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZO06026.1"
                     /translation="TECYGFSLEDIYSFGLKLIRQRWRTIGFPTEEMPPGTFPFDPDA
                     SEQDIAARQVKMMEAGILGPPDGPPPSTDPEMQRIYFDLITRVADPSAANGTPLVYQW
                     RFTDADPWHLIIDNGSTRAEPGEAPNPTVVIESTWADWIAQGKPDANQLKMLLQRRIR
                     PKGSLRELARLRRVFNG"
     gene            complement(536..1075)
                     /locus_tag="VFB52_06545"
     CDS             complement(536..1075)
                     /locus_tag="VFB52_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO06027.1"
                     /translation="MASVTQDVQAVTNLPFKSDPSDVKTFNIGEGMMTDTKAIFHAEG
                     HQTGMALGMVEIIWQQGDATAPHVHKLEDEGFYILEGELTLHLPGRLSVVAQKGEFVW
                     APRDMPHYYQVTGEDGARVLVLEIPGGSLMTFFRGVAEGMGSDISTDEKLNEFAEWSD
                     DNFGIKFFEPDHDFGTPEW"
     gene            complement(1163..1918)
                     /locus_tag="VFB52_06550"
     CDS             complement(1163..1918)
                     /locus_tag="VFB52_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZO06028.1"
                     /translation="MSRPVLVWIADALSPWRAPDAEARLARELAARSGWSLESVADEE
                     GEGSGTFPQPLSHAYRRLGELQARVGEAAPVAIGGCGAGAGIAAAVALLTQRYDEEPP
                     AATVLLAPLLDARLASPSWPRFADAAERWQMEAALAAYAPGLDRSDPLLSPLLAERLE
                     GLPTTAIVTAAEDPMRDDGERYAERLRAAGVTVVGHRYPGTDHTQACGGGTSAEGAAV
                     VSGLAAALRLILSRSTQFGGASSSRSADATHPA"
     gene            complement(1931..2272)
                     /locus_tag="VFB52_06555"
     CDS             complement(1931..2272)
                     /locus_tag="VFB52_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06029.1"
                     /translation="MKNVLMVLSNPAEGEDEAYNDWYTNTHLDEVVSIDGFVSARRYK
                     LTDAQIAGFPGSEHSYLAIYEIEGDPADAFAQLVDELQSGRMELPDSIEQATISPWSF
                     EAISERVAASA"
     gene            complement(2276..>2917)
                     /locus_tag="VFB52_06560"
     CDS             complement(2276..>2917)
                     /locus_tag="VFB52_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO06030.1"
                     /translation="SFYEGSEPPTVNQQLLRDAPSAGPTPLRLRPGTGLPEGGLVPYQ
                     PVSNKPFKSDPAKSEIMQIGRGMMTDVRMIFHAFGFQTGNTFGLIEIDWGPGDVAGPH
                     VHHLEDEGFFVLEGELTMHVAGVGEVPARAGEFVWAPRGIPHYYSVTGERGARVLVFE
                     VPGGTLMDFFYRAATEQRGADIESDEDLQRFVEWSSHNFGIDFMDPEMFPEEG"
BASE COUNT          442 a         1061 c          968 g          446 t
ORIGIN      
        1 caccgagtgc tacggcttct cgctcgagga catctactcc ttcgggctca aactgatccg
       61 tcagcgctgg cgcacgatcg gcttcccgac cgaggagatg ccgcccggca ccttcccctt
      121 cgaccccgac gcgagcgagc aggacatcgc cgcccgccag gtgaagatga tggaagccgg
      181 gatcctcggc ccgcccgacg gcccgccgcc ctcgaccgac ccggagatgc agcggatcta
      241 cttcgacctg atcacccgcg tcgccgatcc ctccgccgcc aacggcacgc cgctcgtcta
      301 ccagtggcgc ttcaccgacg ccgacccgtg gcacctgatc atcgacaacg gctcgacccg
      361 cgccgaaccc ggcgaggccc cgaacccgac cgtggtgatc gagagcacct gggccgactg
      421 gatcgcgcag ggcaaaccgg atgccaacca gctgaagatg ctgctgcagc gccgcatccg
      481 ccccaagggc tcgctgcggg agctcgcccg cctgcgcagg gtgttcaacg gctgactacc
      541 actccggcgt gccgaaatcg tggtcgggct cgaagaactt gatcccgaag ttgtcgtcgg
      601 accactcggc gaactcgttt agcttctcat cggtgctgat gtccgagccc atcccttcgg
      661 cgaccccgcg gaagaaggtc atcagcgaac ccccggggat ctcgaggacg agcacacggg
      721 cgccgtcctc tccggtcacc tggtagtagt gcggcatgtc gcggggggcc cagacgaact
      781 cgcccttctg ggcgacgacg ctgagccggc cgggcaggtg cagggtcagc tctccctcga
      841 gaatgtagaa gccctcgtcc tcgagcttgt ggacgtgcgg cgccgtcgcg tcgccctgct
      901 gccagatgat ctccaccatc ccgagcgcca tcccggtctg gtgtccctcg gcgtggaaga
      961 tggccttggt gtcggtcatc atcccctcgc cgatgttgaa ggtcttgacg tcgctcgggt
     1021 ctgatttgaa cggcaggttg gtgactgcct gtacgtcctg cgttacgctc gccacgaact
     1081 gctcctttcc tctgcgccgc ctgcatccct ctggacgaca agcgacgcgc ggcttctcct
     1141 cgatcctcta gcgagcgtag attcaggccg gatgcgttgc atccgccgag cgggacgaac
     1201 tcgccccccc gaactgggtc gagcgcgaca gaatcaggcg cagggcggcc gccaagccgc
     1261 tgaccacagc ggccccctcg gcactcgtcc cgccgccgca agcctgggtg tggtccgtgc
     1321 cgggatagcg gtggccgacc acggtgaccc cggcggcgcg cagccgctcg gcgtagcgct
     1381 cgccgtcgtc ccgcatcggg tcctccgcgg cggtgacgat cgccgtcgtc ggcagccctt
     1441 ccagccgctc ggccaagagc ggcgagagca gcgggtcgga ccggtccagg ccgggcgcgt
     1501 aggccgccag cgccgcctcc atctgccacc gctcggcggc gtcggcgaag cgtggccagg
     1561 agggcgaggc gaggcgggcg tcgagcagcg gcgccagcag gacggtggcg gccggtggct
     1621 cctcgtcata gcgctgcgtg agcagcgcta ccgcagccgc aatcccggcc ccggccccgc
     1681 agcccccgat cgccaccggt gccgcctcgc cgacccgcgc ctggagctcg ccgaggcgac
     1741 ggtaggcgtg gctcagcggc tggggaaagg tcccggatcc ctccccctcc tcgtcggcga
     1801 ccgactccag cgaccaaccc gagcgcgccg ccagctcgcg cgccaggcgc gcttcggcgt
     1861 cgggcgcccg ccacggcgag agcgcgtcgg cgatccagac gaggacggga cggctcacac
     1921 cccccgccgc tcaggccgag gccgcgacgc gctcgctgat cgcctcgaag gaccagggcg
     1981 agatcgtcgc ctgctcgatc gagtcgggca gctccatgcg cccgctctgc agctcgtcga
     2041 ccagctgggc gaaagcatcg gccgggtcgc cctcgatctc gtagatcgcc aggtagctgt
     2101 gctcgctccc ggggaatccg gcgatctggg cgtcggtcag cttgtagcgc cgggccgaga
     2161 cgaagccgtc gatcgagacc acctcgtcga ggtgggtgtt cgtgtaccag tcgttgtagg
     2221 cctcgtcttc cccctcggcg gggttcgaga gcaccatcag cacgttcttc atcggctatc
     2281 cctcctcggg gaacatctcg gggtccatga agtcgatccc gaagttgtgg ctcgaccatt
     2341 cgacgaaccg ctgcagatcc tcgtcggact cgatgtccgc ccctcgctgc tcggtcgcgg
     2401 cccggtagaa gaagtccatc agcgtcccgc cgggcacctc gaagacgagg acgcgggcgc
     2461 ctcgctcgcc ggtcaccgag tagtagtggg gaatcccgcg cggcgcccag acgaactcgc
     2521 cggcccgggc cgggacctcg ccgactccgg ccacgtgcat ggtcagctcg ccctcgagca
     2581 cgaagaagcc ctcgtcctcg agatggtgga cgtgcgggcc ggcaacgtcg ccgggccccc
     2641 agtcgatctc gatcagcccg aaggtgttgc cggtctggaa cccgaaggca tggaagatca
     2701 tccggacgtc ggtcatcatc ccgcgcccga tctgcatgat ctccgacttc gccgggtcgg
     2761 acttgaaggg cttgttggag accggctggt aggggaccag cccaccctcg ggaagcccgg
     2821 tgcccggccg caaccgcagc ggcgtcgggc cggcggaagg tgcgtcgcgg agcagctgct
     2881 ggttgacggt cggcggctcg gagccctcgt agaagga
//
LOCUS       DASRIG010000239         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_370016, whole genome shotgun sequence.
ACCESSION   DASRIG010000239 DASRIG010000000
VERSION     DASRIG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370016"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFB52_06565"
     CDS             <1..298
                     /locus_tag="VFB52_06565"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HZO06031.1"
                     /translation="HDVTATTDDPGLRGLSDSDLFEVAQQQGRALVTYNRVDFEPIIR
                     EYAQLNREHRGLVIVHPTRFPSWEFARLAEAIEGLLEGPELGIGFLVWLAPPRA"
     gene            complement(309..719)
                     /locus_tag="VFB52_06570"
     CDS             complement(309..719)
                     /locus_tag="VFB52_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06032.1"
                     /translation="MSATTKWITLALLGLAIAAAVAFLASRLVSEQIGIASESVSAGY
                     DLAPAVRSATAPNTKTGNKDPTTPSDTTQTTPPESTTDSPSTTGDDAVDPDQLADELE
                     DREKEREDELKDLEKEREDREDEIRDEEEDRRDD"
     gene            complement(716..2044)
                     /locus_tag="VFB52_06575"
     CDS             complement(716..2044)
                     /locus_tag="VFB52_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO06033.1"
                     /translation="MLLLALIALGVPLALSFRDRVDTEVRSQAESQAALVATSASEVI
                     APPRPGVLERLARVSAETVRGRVIVVDSRGRLLADSAGVPLGESYADRPEIAGALDGH
                     DEQISRESATLDEEILATAVPVVQRGQTAGAVRITQSVEAVDGAVRNSILNVLGLGAV
                     VLALALIAGALIAQQIARPIRRLDRTAQQVAAGDLDASAPVEGSKEQRSLARSFNEMT
                     QRLKRLLSVQQDFVADASHQLRTPLTGLRLRIENLGASHEGDIALEGEVDASLREVDR
                     LSLIVEELLILSRAGESELPGEQLDLADAVRRAGSRWEAAARERRIALAVETSEPAPG
                     WCAGPDLDRTIDVLIENALLYSPPSSTVTITGAPGRVAILDEGPGLAEGEEQAVFERF
                     ARGTAGRRVPSGTGLGLPIARELARSWGGDVNLSNRAQGGLCAVIELEDK"
     gene            complement(2080..>2761)
                     /locus_tag="VFB52_06580"
     CDS             complement(2080..>2761)
                     /locus_tag="VFB52_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06034.1"
                     /translation="VEDEESISQPFAEALRRAGFEPLVTATAAGALELAASEQPDLVM
                     LDLALPDGDGRDVCRELRRDSDVPIMMLTARGTELDKIVGLELGADDYVVKPFSAAEV
                     ISRIRAVLRRSAPRPPREEQPLQVGDLDLDPAARIATLAGEQLDLSRKEFDLLAALMR
                     NAGRVVKREELMSEVWDSNWFGSTKTLDVHIGWLRRKLGDEATRPGYIETVRGVGFRF
                     AAPEAEAP"
BASE COUNT          339 a          919 c         1046 g          457 t
ORIGIN      
        1 ccacgacgtc accgccacca ccgacgatcc aggtctccgg ggcctgagcg actccgacct
       61 gttcgaggtg gcccagcagc aaggccgggc cttggtcacc tacaaccgcg ttgacttcga
      121 accgatcatc cgcgaatacg cccagttgaa tcgcgagcat cgcggcctcg tcatcgtcca
      181 cccgacgcgt ttcccgagct gggagtttgc tcggctggcc gaggcaattg agggcctgct
      241 ggagggtcct gagttgggaa ttggctttct ggtctggcta gcgcccccca gggcgtagct
      301 cgcctcgctc agtcgtcccg gcggtcctct tcttcgtccc tgatttcgtc ttctcggtcc
      361 tcgcgttcct tttctaggtc cttgagttcg tcctcgcgtt ccttttcgcg gtcctcgagt
      421 tcgtcggcga gctggtcggg gtcgacggcg tcgtcgccgg tggtggaggg ggagtcggtg
      481 gttgattcgg gaggggtggt ttgggtggtg tcgctggggg tggtggggtc tttgttgccg
      541 gttttggtgt tgggggcggt ggcggagcgg acggcggggg ccaggtcgta gccggcggag
      601 acggactcgg aggcgatgcc gatttgctcg ctcacgaggc ggctggcgag gaaggcgacg
      661 gccgcggcga tggctaggcc gaggagggcg agggtgatcc atttggtggt ggcgctcatt
      721 tgtcctccag ttcgatcaca gcgcagaggc cgccttgggc ccggttgctg aggttgacgt
      781 cgccgcccca tgagcgggcc agctcgcggg cgattggcag ccctagaccg gtgccgctgg
      841 ggacgcggcg gcctgcggtg ccgcgggcga agcgttcgaa gacggcctgc tcctcgccct
      901 cggccaggcc ggggccctcg tcgaggatcg cgacccggcc gggggcgccg gtgatcgtga
      961 ccgtcgagct gggcggtgag tagagcaggg cgttctcgat taggacatcg atggtgcggt
     1021 cgaggtcggg gccggcgcac cagccggggg ccggctcgct cgtctctacc gcgagggcga
     1081 tgcggcgctc gcgggcggcg gcttcccagc gggatcccgc tcggcggacg gcgtcggcga
     1141 ggtcgagctg ctcgccgggg agttcgctct ctcccgcgcg gctcaagatc aacagctcct
     1201 cgacgatcag ggacagccgg tcgacctcgc gcagggacgc gtcgacctcg ccttcgaggg
     1261 cgatgtcgcc ctcgtggctt gcccccaggt tctcgatccg caggcgcagc ccggtcagcg
     1321 gcgtgcgcag ctggtgggag gcatcggcga cgaagtcctg ttggacgctc agcagtcgct
     1381 tcagcctctg cgtcatctcg ttgaagctgc gcgccagcga gcgctgctcc ttgctgcctt
     1441 cgaccggcgc cgacgcatcg aggtcgccgg cggcgacctg ctgcgcggtc cggtcgaggc
     1501 ggcggatcgg gcgcgcgatc tgctgggcga tcagggcacc cgcgatcagg gccagggcga
     1561 gcacgaccgc gcccaggccg agcacgttga ggatcgagtt gcgcacggcc ccgtcgaccg
     1621 cctcgacgct ctgggtgatc cgcacggccc cggcggtttg cccgcgctgg accaccggca
     1681 ccgcggtcgc caggatctcc tcgtccagcg tcgcgctctc gcggctgatc tgttcgtcgt
     1741 ggccgtcgag cgcgccggcg atctcgggcc ggtcggcgta gctctcgccg agcgggacgc
     1801 cggcggagtc ggcgagcagg cggccgcggc tgtcgacgac gatcacgcgg cctcgcacgg
     1861 tctcggccga gacgcgggcc agtcgttcca gtacccccgg ccgcggcggc gcgatcactt
     1921 ccgaggcgga ggtggcgacg agcgcggcct ggctctccgc ctggctgcgg acctcggtgt
     1981 cgacgcggtc gcggaagctc aatgccagcg gcacgccgag cgcgatcagc gccagcagca
     2041 gcacgtaggc gagggccagc aggagggtcg accggaggct tacggtgcct cagcctccgg
     2101 ggcggcgaag cggaagccga cgccgcgcac ggtctctata tacccgggcc gcgtggcctc
     2161 gtcgccgagc ttgcggcgca gccagccgat gtggacgtcg agcgtcttgg tcgagccgaa
     2221 ccagttgctg tcccagacct ccgacatcag ctcctcgcgt ttgaccacgc ggccggcgtt
     2281 gcgcatcagc gcggccagca ggtcgaactc cttgcgggag aggtcgagct gctcgcccgc
     2341 gagcgtggcg atccgggcgg ccgggtcgag gtcgaggtcg ccgacctgca gcggctgctc
     2401 ctcgcgcggc gggcgcgggg cgctgcggcg caggacggcg cggatccgcg agatcacctc
     2461 ggcggcgctg aacggcttga ccacgtagtc gtcggcgccg agctcgaggc cgacgatttt
     2521 gtccagctcg gtgccgcgcg cagtcagcat catgatcggc acgtcggagt cgcggcgcag
     2581 ctcgcggcag acgtcgcgcc cgtcgccgtc gggcagcgcg aggtcgagca tcactaggtc
     2641 cggctgctcg ctggcggcga gctccagcgc cccggccgcg gtggccgtga ccaggggttc
     2701 gaagccggcg cggcgcagcg cctcggcgaa cggctgggag atcgactcct cgtcctcgac
     2761 g
//
LOCUS       DASRIG010000240         6686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1173133, whole genome shotgun sequence.
ACCESSION   DASRIG010000240 DASRIG010000000
VERSION     DASRIG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173133"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..169
                     /locus_tag="VFB52_06585"
     CDS             5..169
                     /locus_tag="VFB52_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO06035.1"
                     /translation="MAEGRTNAAIAAELVVTPRAVEKHVSNIFWKLDLPASPEDHRRV
                     LAVLAWTEAR"
     gene            complement(166..1233)
                     /locus_tag="VFB52_06590"
     CDS             complement(166..1233)
                     /locus_tag="VFB52_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZO06036.1"
                     /translation="MIYAAPPITDSLASRLDDLGTLRDALGDRIATRSRWLGSLRREI
                     RASAFSGSTAIEGFTVSLDEARELTRGGGAPSRGDEHREALACYTRAMDHVGTMAIDP
                     SFRWLDRVILDLHFEACYFQPESEPGLWRKVGVAIFADDGSTAFSAPASERVAGLMAE
                     LIEWLADGDLDADPFVRAAMAHLNLISVHPFRDGNGRISRIIQSLVLAQAGFGAPEFF
                     SIEEYLGSHTPEYYAALREAQGGSFQPERDATGWVELCLDAHLVQARQRLDQIKQAGE
                     RWQRLEDLVDSRGWPDRLVIALEQSLVGGTDRGRYGEEAGISEATASADFRRLLDAGL
                     VRQLGRGRNTRYVASDELRAR"
     gene            complement(1289..1555)
                     /locus_tag="VFB52_06595"
     CDS             complement(1289..1555)
                     /locus_tag="VFB52_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06037.1"
                     /translation="MPQPRRGKKQEQGDYGWVISFGCKNTGTLKRLKVDTDPSSDTSP
                     IEVPCECGETHRVTVSPYRRRRAADETFDLEVKSLDELRGKSAD"
     gene            complement(1568..2452)
                     /locus_tag="VFB52_06600"
     CDS             complement(1568..2452)
                     /locus_tag="VFB52_06600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZO06038.1"
                     /translation="MLVRPQGNRTRQLTAATVAALLALALAFVVQASQAAAASLRYVA
                     LGDSYSAASGVLPLDLTAPLQCLRSTRNYPHVIASDIDAQLTDVTCGAAETSHYFEPQ
                     YPGMAPQLDAVKANTELVTMTIGGNDSGVFINAILSCGTAGLLTLGQGSPCKDRYGTS
                     FEDTIRDVTYPSLVKALGAVQAKAPSAEVAILGYPWIMPASGSCFTKMPVAVGDVPYV
                     RGIQATLNDAVRRAALATGARYVNMNKVSNGHDACQAIGTRWVEPVLQGTNPVIVHPN
                     ALGEREMAAQAIKVLGLD"
     gene            2528..3244
                     /locus_tag="VFB52_06605"
     CDS             2528..3244
                     /locus_tag="VFB52_06605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO06039.1"
                     /translation="MAGMIEAVTFDFWNTIARVRGQMVAARVRTVGAVLAQGEREVEP
                     EELTAALERVAAHWERSWEAGTHLHPRDGAAMLVRELGVEGAAEELVAEAFLNAGSEA
                     ELEIAPGLGPVLAELDGRGVRLGIVCDVGFAGGEQLRALLAREGLLTHFSGWGFSDEV
                     GHYKPSPQIFEAALAALGAPPERALHVGDLRRTDVAGAAALGMGTVRYRGLHDDLDSA
                     PVEAEHVLDDHRGLLEVVGG"
     gene            3237..4595
                     /locus_tag="VFB52_06610"
     CDS             3237..4595
                     /locus_tag="VFB52_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZO06040.1"
                     /translation="MAEGAPAAGALAEWSAGLAYADLPAPVAEAAKLHLLDAVGAGLA
                     GLALDQLPAARAVALEMGGEEEASGLGLPRRVGTAAAALANGSLMHALDFDDTHEVAL
                     VHSSAVVAAAALAVGEATGASGEELIAASVAGYEISSRVGLANPGALHLKGFHPTSVC
                     GVFAAAAIAAKLRGLSAEQTTNALGIAGSQASGLMEFLADGSQTKPLHAGWAAHAGIV
                     AAALAAHGATGPATVLEGRFGLLASHLGEFDESGLAGELGERWETTAIAFKPYPTCHC
                     THAVLDAVAATGIGAEGIEEIVAVVPGEVAVGLVLEPSERKARPRTPYDAKFSLPYCI
                     GVLLTRGEVGVESFTADALDDEAALAIAAKVSYVVEEFEGGNDLSGGVRARVGGEEFE
                     ASVLRPRGGPANPIAADELRAKFLRNARLALGEQRAEELAEVMLGFERYPADAVAKFL
                     AP"
     gene            4658..5197
                     /locus_tag="VFB52_06615"
     CDS             4658..5197
                     /locus_tag="VFB52_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06041.1"
                     /translation="MAAAVIGVGVLGPAIAVIGLIWLVTYTLRRVGVNPPEPPRPRLS
                     IAWLGEFFDYLGRLIVWAAPIVPFVAPAITFVAVTWPLPKESDGLLFQEQASQILLVL
                     LIGFVIEAGALRWRRQAVDWLLSLVTVLILLVGEIYTLVALASNDPNHADIIAGAMAA
                     GIVAILTAAITGAAKPAEE"
     gene            5263..5511
                     /locus_tag="VFB52_06620"
     CDS             5263..5511
                     /locus_tag="VFB52_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06042.1"
                     /translation="MAKDVSIRELRNSTAAVISELEAGERLTLTVNRRPVADILPHVE
                     QRDPWVASGELRRIVREAPADLGLLDDLADIRGDELDD"
     gene            complement(5898..>6686)
                     /locus_tag="VFB52_06625"
     CDS             complement(5898..>6686)
                     /locus_tag="VFB52_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06043.1"
                     /translation="DHNYIVQRSPVLADRLGARMQNGIENVAAEIARVLNGDQASDEK
                     GRDPAAEGEPGALLSIPSSLAAQEELARQRPDWWEYRLFAGVLVEGLLRLEGKWQDHQ
                     LRLPGPERRYVDPNHPTQFFSQELSWVRRQLVVERIFGSEVLNQAFGEPGEPGDPERI
                     SHVVRRLIQMYEGLMDWAAGLRNANVPDQFEELVELYAQMVDLPLVQIRDFVQHAADQ
                     IAQLSILAQDGTEEEPVIITLDLTLSADEALMSEVDSALHRLSH"
BASE COUNT          948 a         2264 c         2358 g         1116 t
ORIGIN      
        1 cctcatggcc gagggccgca ccaacgccgc catcgccgcc gagctggtcg tcaccccgcg
       61 cgcggtcgag aagcacgtct ccaacatctt ctggaagctc gacctcccgg ccagccccga
      121 ggaccaccgc cgcgtcctcg ccgtgctcgc ctggaccgaa gcgcgttagc gcgcgcgcag
      181 ctcgtcgctg gcgacgtagc gcgtgttgcg cccgcggccg agctggcgca ccagcccagc
      241 gtcgagcagg cggcggaagt cggcgctcgc ggtggcctcg gagatcccgg cctcctcgcc
      301 gtacctgccg cggtcggttc ctccgaccag gctttgctcc agggcgatca cgagtcgatc
      361 gggccagccc cgggagtcga ccaggtcttc gagccgctgc cagcgctcgc cggcctgctt
      421 gatctggtcg aggcgctgcc gcgcctgcac gaggtgggcg tcgaggcata gctcgaccca
      481 gccggtggcg tcgcgctctg gttggaagct gccgccctgc gcctcgcgga gggcggcgta
      541 gtactccggc gtgtggctgc cgaggtactc ctcgatcgag aagaactcgg gggcaccgaa
      601 gccggcttgc gcgaggacca gcgactggat gatccgcgag atccggccgt tgccgtcgcg
      661 aaaagggtga acggagatca ggttgaggtg cgccatcgcc gcgcgcacaa acgggtccgc
      721 gtcgagatcg ccgtcggcga gccactcgat cagctcggcc atcagtccgg ctacccgctc
      781 ggaagccggt gcgctgaacg ccgtcgagcc atcgtcggcg aagattgcga caccgacctt
      841 gcgccagagc ccgggttcgg attcaggctg gaagtagcag gcctcgaagt ggaggtcgag
      901 gatcacccgg tcgagccagc ggaacgaggg gtcgatcgcc atcgtcccca cgtggtccat
      961 tgcgcgcgtg taacaggcca gcgcttcgcg gtgttcgtcg ccgcggctgg gcgctccgcc
     1021 tccacgggtc agctcgcggg cttcgtcgag gctgaccgtg aatccctcga tcgcggtcga
     1081 gccggagaac gcgctcgcgc ggatctcgcg acgcagcgat ccgagccagc gcgacctggt
     1141 tgcgatccgg tcgccgaggg cgtcgcggag cgtgccgaga tcgtcaagtc ggctggcgag
     1201 agagtcggtg atagggggag cggcatagat catgctcccg attgtagacg attaaacgtt
     1261 taatcgtcta aacgcaattg cgtatgagtc aatccgcgga tttcccgcgc agctcgtcga
     1321 gcgacttgac ctcgaggtcg aaggtctcgt cggcggcgcg gcggcggcgg tagggagaaa
     1381 cggtgacgcg gtgggtttca ccgcactcgc aggggacctc gattgggctg gtgtccgagg
     1441 acgggtcggt gtcgactttc agccgcttca gcgtgccggt gtttttgcag ccgaaggaga
     1501 tcacccagcc gtagtcgccc tgctcctgtt tttttccgcg ccggggttgg ggcatcagcc
     1561 ccgcacccta atcgagcccg aggactttga tcgcctgggc ggccatttcg cgctcgccga
     1621 gcgcgttcgg gtgcacgatc accgggttgg tgccctgcag gaccggctcg acccagcggg
     1681 tgccgatcgc ctggcaggcg tcgtggccgt tggagacttt gttcatgttc acgtacctcg
     1741 ccccggtcgc cagcgcggcg cggcggacgg cgtcgttgag ggtcgcctgg atcccgcgca
     1801 cgtagggcac gtcgccgacc gcgaccggca ttttcgtgaa gcagctgccg ctcgcgggca
     1861 tgatccacgg gtagccgagg attgcgacct cggcgctcgg cgccttcgcc tgcacggcgc
     1921 ccagcgcttt caccagcgag gggtaggtga cgtcgcggat cgtgtcctcg aaggaggtgc
     1981 cgtagcggtc tttgcagggg ctgccctggc cgagcgtgag caggccggcg gtgccgcagg
     2041 agaggatcgc gttgatgaag acgccggagt cgttgccgcc gatcgtcatc gtcaccagct
     2101 cggtgttcgc tttcacggcg tcgagctggg gcgccatccc cgggtactgc ggttcgaagt
     2161 agtggctggt ctcggccgcg ccgcaggtca cgtccgtcag ctgggcatcg atgtcgctgg
     2221 cgatcacgtg cgggtagttg cgcgttgagc gcaggcactg cagtggggcg gtgaggtcga
     2281 gcggcagcac ccccgaggcg gcgctgtagg agtcgccgag ggcgacgtag cgcagcgacg
     2341 cggcggcggc ctgtgaggcc tggacgacga acgccagggc cagcgcgagc agcgcggcga
     2401 cggtggcggc ggtcagctga cgggtgcgat tgccctgcgg gcgcacgagc atcttgctcc
     2461 tcctcggttc cctgtccctg cccgggactt cggcagaaaa tctgccctgc tcggccgatc
     2521 cgcaaacatg gcggggatga tcgaagcggt caccttcgac ttctggaaca cgatcgcccg
     2581 cgtgcgcggg cagatggtcg cggcccgggt gcggacggtc ggcgcggtcc tggcgcaggg
     2641 cgagcgggag gtcgagccgg aggagctgac cgcggcactt gaacgggtcg ctgcccactg
     2701 ggagcgctct tgggaggccg gcacccatct gcacccgcgc gacggcgccg cgatgctggt
     2761 ccgcgagctg ggggtggaag gggcggcgga agagctggtc gccgaggcgt tcctcaacgc
     2821 cggcagcgaa gccgagctgg aaatcgcgcc ggggctgggg ccggtgctgg ccgagctcga
     2881 cgggcgcggg gtgcggctcg ggatcgtctg cgacgtcggc tttgccggcg gcgagcagct
     2941 gcgcgccctg ctcgcgcgcg aagggctctt gacccacttc tccggctggg gcttctccga
     3001 cgaggtcggc cactacaagc cctcgccgca gatcttcgaa gcggcgctgg cggcgctcgg
     3061 ggcgccgccg gagcgggcgc tgcacgtcgg cgacctgcgc cgcaccgacg tcgcaggggc
     3121 cgccgcgctg gggatgggca cggtgcgcta ccgcggcctg cacgacgacc tcgactcggc
     3181 gccggtcgag gccgagcacg tgctcgacga tcaccgcggg ctgctcgagg tcgtcggtgg
     3241 ctgagggcgc gcccgccgct ggggcgctgg ccgagtggtc cgcgggcctc gcctacgccg
     3301 acctgcccgc cccggttgcc gaggccgcaa agctgcacct gctcgacgcg gtcggcgccg
     3361 gcctcgccgg gctggcgctc gaccaactgc cggcggcgcg cgcggttgcc ctggagatgg
     3421 gcggggagga ggaggcgagt gggcttggcc tgccacgccg ggtgggcacc gccgccgccg
     3481 cgctcgccaa cggctcgctc atgcacgcgc tcgacttcga cgacacccac gaggtcgcgc
     3541 tcgtccactc cagcgcggtc gtcgccgcgg cggcgctcgc ggtcggcgag gcgaccgggg
     3601 cgagcggcga ggagctgatc gcggccagcg tcgccggcta cgagatctcc tcgcgggtcg
     3661 ggctcgccaa ccccggcgcg ctgcacctca agggctttca cccgacctcg gtctgcggcg
     3721 tcttcgccgc tgccgcgatt gcggcgaagc tgcgtgggct gagcgccgag cagacgacta
     3781 acgcgctcgg gatcgccggt agccaggcct cggggctgat ggagtttctc gccgacggct
     3841 cgcagaccaa gccgctgcac gccggctggg cggcgcacgc ggggatcgtc gccgcggcgc
     3901 tcgccgccca cggcgcgacc gggccggcga ccgttctgga gggccgcttc ggcctgctcg
     3961 cctcgcacct cggcgagttc gacgagtccg gcctcgccgg cgagctcggc gagcgttggg
     4021 agacgaccgc gatcgccttc aagccctacc cgacctgcca ctgcacccac gcggtgctcg
     4081 acgcggtcgc cgcgaccggg attggcgccg aggggatcga agagattgtc gcggtcgtcc
     4141 ccggcgaggt cgccgtcggc ttggtcctcg agccgagcga gcgcaaggcg cgcccgcgca
     4201 ccccctacga cgccaagttc agcctcccgt actgcatcgg ggtgctcttg acccgcggcg
     4261 aggttggcgt ggagtccttc accgccgacg cgctcgacga cgaggcggcg ctggcgatcg
     4321 ccgccaaggt ctcttatgtg gtggaggagt tcgagggcgg caacgacctc tccggcggcg
     4381 ttcgcgcccg ggtcgggggc gaggagttcg aggcgagcgt tctgcgcccg cgcggcggcc
     4441 cggccaaccc gatcgcggcg gacgagctgc gcgccaagtt cctccgcaac gcccgcctcg
     4501 cgttgggcga gcagcgcgct gaggagctgg ccgaggtgat gctcggattc gagcgctacc
     4561 cggccgacgc cgtcgctaag ttcctggcgc cctgaacgag cgccagccaa gcaagcagcg
     4621 caacgcggtc ggctctgtcc tcctggccgt cgtctccgtg gccgccgcgg tgatcggggt
     4681 gggggtgctg ggacccgcga tcgccgtgat cggcctcatc tggctggtca cctacacgct
     4741 gcgccgggtc ggcgtgaacc cacccgagcc gccgcgcccg cgcctctcga tcgcctggct
     4801 cggagagttc ttcgactacc tcggccggct gatcgtctgg gccgccccga tcgttccgtt
     4861 cgtcgccccc gcgatcacct tcgtcgccgt cacctggccg ctgccgaagg aatccgacgg
     4921 cctgctgttc caggaacagg cctcccagat cctgctcgtg ctgctgatcg gcttcgtgat
     4981 cgaggccggc gcgctgcgct ggcgccgcca ggcggtcgac tggttgctca gcctcgtcac
     5041 cgtcctgatc ctcctggtcg gcgagatcta caccctcgtc gccctcgcca gcaacgaccc
     5101 caaccacgcc gacatcatcg ccggcgcgat ggccgccggc atcgtcgcga tcctcaccgc
     5161 cgcgatcacc ggcgccgcca agcccgccga ggagtagggc cgtgccaggc ccgcgggtcg
     5221 acccacgggg cggggtgtag cacgcagtgc tacagtcgtg aaatggcaaa ggacgtatcc
     5281 attcgtgagc tgcgaaattc caccgcggca gtgatctccg aactggaagc gggtgaacgt
     5341 ctgactctga ctgtgaaccg caggccagtc gcagatatcc ttccccatgt cgagcagcgc
     5401 gatccctggg tggcttcagg cgagctgcgt cgaatcgtgc gtgaggcgcc cgccgacctc
     5461 ggcctgctcg acgacctcgc cgatattcgc ggggacgagc tcgacgattg acacgggcgg
     5521 tggccgacac ctccgtgttc gtggcacagg agaccggccg agagcttggg gatctccctg
     5581 agcggattgc ggtctcggtc atcaccgccg ctgagctcga gctgggagtt ctccgcgcgg
     5641 ccgagcccga gtcgagagcc ctccgcctcg caaccctgtc gagggttcaa gcgacgtatc
     5701 cgctgctgcc cgttgatgct gaggttgcga gctgttttgc gcgactcgcc gcagcggagc
     5761 gtgctcgtgg ccgccgcctt cgccgtcatg acacttggat agccgctacc gccttgcgcc
     5821 acgatgctgt cgtggttact caagactccg acttctcttc gtttgaggac atcgaggtcc
     5881 taagggtctg aacagcgtca gtgagagagt cgatgcagcg ctgagtcaac ttcactcatt
     5941 agtgcttcgt ccgccgaaag cgtcagatct aaggtgatta tcactggctc ctcttcggtg
     6001 ccgtcttggg cgagaatcga cagctgggcg atttggtcgg ctgcgtgttg gacgaaatcg
     6061 cggatttgca ccaagggcag gtcgaccatt tgtgcgtaca actccaccag ttcctcgaac
     6121 tgatcgggga cgttggcatt ccgcagtccc gccgcccagt ccatcaagcc ctcgtacatc
     6181 tgaatcaagc gtctgacgac atgtgagatc cgctccggat cccctggctc gcctggttcg
     6241 ccgaatgcct gatttaggac ttcggagccg aagatccgct ctacgacgag ttgccttctt
     6301 acccaagaga gctcctggct gaagaactgc gtgggatggt tggggtcgac atagcggcgc
     6361 tctggacccg gaagtctgag ttggtggtct tgccacttgc cttccaacct caataggccc
     6421 tcgaccagaa cgccagcgaa caggcgatac tcccaccagt cggggcgctg cctagcgagt
     6481 tcttcctgag ccgcgaggga ggacggaatc gacagcaatg cgcctggctc gccctcggcg
     6541 gcaggatcgc gtcccttctc gtcgctcgct tggtcaccgt tcagtacccg cgcgatttca
     6601 gccgccacgt tctcgattcc attctgcatt ctcgctccca ggcggtctgc gagaaccggg
     6661 gaccgctgca cgatgtagtt gtggtc
//
LOCUS       DASRIG010000241         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_370977, whole genome shotgun sequence.
ACCESSION   DASRIG010000241 DASRIG010000000
VERSION     DASRIG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370977"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFB52_06630"
     CDS             complement(<1..930)
                     /locus_tag="VFB52_06630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZO06044.1"
                     /translation="MSTIEDRPQVDTGMEHDYRKRMMVFGGRSSMDLAAKVAGKLELD
                     LGQVTLKTFADGEVYCRYEESIRGADVFLVQSTCANEQTGMTPNDSLVELLTMIDAAQ
                     GASAHRIIAVMPWYGYARQDKKSAPREPITARIVAKTLEAVGVDRVLTMDLHAGQVQG
                     FFHVPVDHMTAYPMLTQWFIDQEMKDELVIVSPDAGRVKTARNFARRVGTQWAVMEKE
                     RPAQQVAEIGYVVGDVKGKTAVLVDDMIDTAGTLCAAAQTVLDEGAARVIACATHGVF
                     SGPAYERLAYESSGIEQIVVTDTLPLRPGAPDNI"
     gene            complement(927..>2183)
                     /gene="glmU"
                     /locus_tag="VFB52_06635"
     CDS             complement(927..>2183)
                     /gene="glmU"
                     /locus_tag="VFB52_06635"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZO06045.1"
                     /translation="GRVAAIVSPGRDISAGLPEETETVEQPEADGTGGAIRAALPLIE
                     EAEHVLVLSGDVPLISTETIRQLLEAHLASPAAATMLTIELDDPGSYGRVVRGESGEV
                     ERVVEAKAAGDADPEQLAIREINAGTYAFDAAPLKEALEGLSNDNAQGEYYLPDVFPA
                     LREAGYSVAAHLADDPAVTMGVNNRVDLAAVETEARRRLLEGHMLAGVTVVDPGSTWI
                     DADVEIAADARLEPGTSLRGATTVGANSVIGPLTTLIDTGVGVDCAIPHSYLVECEVR
                     DACSVGPFAYLRPGALLDAGAKVGTFVEVKNSHIGKGAKVPHLAYVGDADVGGGSNLG
                     AGTITANYDGFRKHRTVIGRDVRIGVDTMLIAPVEVGDSAYTGAGAVVKSDVPEGSLA
                     VSENAQRNIDGYAADKAAKMREEESP"
BASE COUNT          290 a          739 c          793 g          361 t
ORIGIN      
        1 gatgttgtcg ggcgcgccgg ggcgcagcgg aagcgtgtcg gtgacgacga tctgctcgat
       61 cccggagctc tcgtaggcga ggcgctcgta ggccggcccg gagaagacgc cgtgggtggc
      121 gcaggcgatg acccgcgcgg cgccctcgtc gagcacggtc tgggcggcag cgcagagggt
      181 gcctgcggtg tcgatcatgt cgtcgaccag gaccgcggtc ttgcccttga cgtcgccgac
      241 cacgtagccg atctcggcga cctgctgggc gggccgctcc ttctccatca ccgcccactg
      301 ggtgccgacc cggcgggcga agttgcgcgc cgtcttcacc cggccggcgt ccggggagac
      361 gatcaccagc tcgtccttca tttcctggtc gatgaaccac tgggtcagca tcggatacgc
      421 cgtcatgtgg tcgaccggga cgtggaagaa gccctggacc tgaccggcgt gcaggtccat
      481 ggtgagcacg cggtcgaccc ccaccgcctc cagcgttttg gcgacgatgc gagcggtgat
      541 cggctcacgg ggagccgact tcttgtcctg gcgcgcgtac ccgtaccagg gcatcacggc
      601 gatgatccgg tgggcggagg cgccctgggc ggcgtcgatc atcgtcagca gctcgaccag
      661 cgagtcgttc ggggtcatcc cggtctgctc gttggcgcag gtcgactgca cgaggaagac
      721 gtcggcgccg cggatcgact cctcgtagcg gcagtagacc tcgccgtcgg cgaaggtctt
      781 cagcgtgact tgaccgaggt cgagttcgag cttgccggcg accttggcgg caaggtccat
      841 cgagctgcgg ccgccgaaga ccatcatccg tttgcggtag tcgtgctcca tgccggtatc
      901 cacctgcggg cgatcctcga tcgtgctcac gggctttctt cctctcgcat cttggccgcc
      961 ttgtcggcgg cgtatccatc gatgtttcgc tgcgcgttct cgctcacggc aagtgagccc
     1021 tcggggacgt cggacttgac caccgcgcca gcgccagtgt aggcggagtc gccgacttct
     1081 accggcgcga tcagcatcgt gtcaacgccg atccggacgt cgcgcccgat caccgtccgg
     1141 tgcttgcgga aaccgtcgta gttggcggtg atcgtgccgg cgccgaggtt gctgccgccg
     1201 cctacgtcgg cgtcgccgac gtaggccagg tgcggcacct tggcgccctt gccgatgtgc
     1261 gagttcttga cctcgacgaa ggtcccgacc ttggcgccgg cgtcgagcag ggcgccgggg
     1321 cgcaggtaag cgaagggacc gacgctgcag gcgtcgcgga cctcgcactc gaccaggtag
     1381 gagtgcggga tcgcgcagtc gaccccgacc ccggtgtcga tcagcgtggt cagcgggccg
     1441 atcaccgagt tggcgccgac cgtggtcgcg ccgcgcaggc tggtgccggg ctcgaggcgg
     1501 gcgtcggcgg cgatctcgac gtcggcgtcg atccaggtcg agccggggtc gacgacggtg
     1561 acgccggcca gcatgtggcc ctcgagcagg cggcggcgcg cctcggtctc gaccgcggcg
     1621 aggtcgacgc ggttgttgac ccccatcgtc accgcggggt cgtcggcgag gtgggcggca
     1681 accgagtagc cggcctcgcg cagggccggg aagacgtcgg gcaggtagta ctcgccctgg
     1741 gcgttgtcgt tggagagccc ctccagggcc tccttcagcg gagcggcgtc gaaggcgtag
     1801 gtgccggcgt tgatctcgcg gatcgccagc tgctcgggat cggcgtcgcc ggccgctttc
     1861 gcctcgacca cgcgctcgac ctcgccggac tcgccgcgaa ccacgcggcc gtaggagccg
     1921 gggtcgtcga gctcgatcgt cagcatcgtc gccgccgcgg gcgaggcgag gtgcgcctcg
     1981 agcagttggc ggatcgtctc ggtcgagatc agcggcacgt cgccggagag gaccagcacg
     2041 tgctcggctt cctcgatcag cggcagtgcc gcccggatcg cgccgccggt gccgtcggcc
     2101 tccggctgct cgaccgtctc ggtctcctcg ggcagcccgg cggagatgtc gcgcccaggc
     2161 gagacgatcg ccgcaacccg ccc
//
LOCUS       DASRIG010000242         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3787295, whole genome shotgun sequence.
ACCESSION   DASRIG010000242 DASRIG010000000
VERSION     DASRIG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3787295"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFB52_06640"
     CDS             <1..294
                     /locus_tag="VFB52_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06046.1"
                     /translation="AANPIWAPKALGRPRLAGAPIVGRTVTCVVRWLVRPTRQLAYSF
                     VADGLQVQSGARRTYRVRAADLGKRLACDAQGATAGGRGGTASLSSPRLVRSG"
     gene            522..836
                     /locus_tag="VFB52_06645"
     CDS             522..836
                     /locus_tag="VFB52_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06047.1"
                     /translation="MGLALVVLVGSFVLRPSPVLGVDGTALGSSVPNRGGFLLGSLPP
                     CEQRAGNHWICSVYDSGFSGTIEYDVRVGSLGCWRAVPAERRPGAESFSGCVSVLNYF
                     LP"
     gene            complement(817..1287)
                     /locus_tag="VFB52_06650"
     CDS             complement(817..1287)
                     /locus_tag="VFB52_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO06048.1"
                     /translation="MSRRAPDTAARQQPENLGVLLREPFLAASEDLHRRFAERGHDQV
                     RPPHGNVFQFLDGAGTRVSVLAERAQVTKQSMAELVAHLERHGYVERTPDPSDRRAKL
                     VRATPRGEELYALAREFVAETEAEWGRKLGKRKLAQLRELLEELNDAVVRARSS"
     gene            1366..1725
                     /locus_tag="VFB52_06655"
     CDS             1366..1725
                     /locus_tag="VFB52_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO06049.1"
                     /translation="MRDAVKVGSDVAEFRVTSEQSAGALVAYEVTIPPGGGPPHMHSH
                     EAFELFRVESGELAFYLEDDGETRRSLAAPGEVVAIPSGREHTVRNESAEEARAFAVV
                     SPGAEMERFMRSLGEPA"
     gene            1725..2141
                     /locus_tag="VFB52_06660"
     CDS             1725..2141
                     /locus_tag="VFB52_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZO06050.1"
                     /translation="MATLTATHAPAELLAPLQSSWAMLLKTRKRDGNWVATPVNVAVD
                     GDRAYFGTPADSGKVKRLRNFDAVEVAPCTVRGKPTGPPLAMRARRLEGAESEAARTR
                     LLAKYPLVHRFLVPLELRLKRVSEVRYELEVDDLQV"
     gene            complement(2115..>2669)
                     /locus_tag="VFB52_06665"
     CDS             complement(2115..>2669)
                     /locus_tag="VFB52_06665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZO06051.1"
                     /translation="AERFAIDFVQLTPANRLVDGPIDMLTSYPYFGATIYSASKGKVV
                     GVVDGIPETPAGTFPKNIGAAEAGGNHIVVDMGGGRYAFYAHLQPGSAMVKVGQRVQA
                     GQPIGRLGNSGNSDGPHLHFHVMDGPSPLASDGVPYRFTRFSVRGTVTNLVPLTEGAV
                     AAIDPQPTGTRARMLPLDLQIVDF"
BASE COUNT          339 a          949 c          975 g          406 t
ORIGIN      
        1 gccgccaacc cgatctgggc accgaaagcg ctcggccgcc cgcggctagc cggcgccccg
       61 atcgtcggcc gcaccgtcac ctgcgtggtg cgctggctgg tccgcccgac ccgccagctc
      121 gcctactcgt tcgtcgccga cggcctgcag gtgcagagcg gcgcccgccg cacctaccgc
      181 gtccgcgccg ccgacctcgg caagcgcctc gcctgcgacg cccagggcgc caccgccggc
      241 ggtcgcggcg gcaccgcctc gctctcgtcg ccgcgcctgg tccgcagcgg ctgacaccct
      301 cgcggcgctt gcaagttcct gcgcgttctg cacaattccc gggatgtcgt gggggctcgc
      361 actctggatc ccgctcgtcg gcgcgatcgc gctggccgcg ctgacgctcg ccccggccgc
      421 ggtgctacgc ccagcgcctc tactcgcccg agcagagcga gcgcgcgaag ctgcccttcc
      481 tgcgccagcg ctcgcgccga accttcgccg cctgggtcgc ggtggggctg gcgctcgtcg
      541 tcctcgtcgg ctccttcgtc ctccgccctt caccggtgct tggcgtcgac ggcaccgcgc
      601 tcggcagctc ggttcccaac cgcggcggct tcctgctcgg ctccctgccg ccctgcgagc
      661 aacgggccgg caaccactgg atctgcagcg tctacgactc cgggttcagc ggcacgatcg
      721 agtacgacgt tcgcgtcggc tcgctcggct gctggcgggc ggtgcccgcc gaacgccggc
      781 cgggagcgga gagcttctcc ggctgcgtct cggtcctcaa ctacttcttg ccctgaccac
      841 cgcgtcgttc agctcttcga gcagctcgcg cagctgcgcg agcttgcgtt tgccgagctt
      901 gcgcccccac tcggcttcgg tctcggcgac gaactcgcgg gcgagcgcgt agagctcctc
      961 cccgcgcggg gtcgcgcgga ccagcttggc gcggcggtcg ctcgggtcgg gggtgcgctc
     1021 gacgtagccg tggcgttcga ggtgggcgac cagctcggcc atcgactgct tggtcacctg
     1081 ggcgcgctcg gcgagcacgc tgaccctggt ccccgcaccg tcgaggaact ggaagacgtt
     1141 gccgtgcggc gggcggacct ggtcgtggcc gcgctcggcg aagcggcggt gcaggtcctc
     1201 gctggcggcg aggaacggtt cgcgcaacag cacgccgagg ttctcgggct gctggcgggc
     1261 ggcggtgtcg ggggcgcgtc ttgacattgg acaggtgtcc tgtctatcct accgagcgcg
     1321 acagacaggg tccctgtcta ttgaccgaga cgaggagatg aacaaatgag agacgcagtg
     1381 aaggtcggca gcgacgtcgc cgagttccgg gtgaccagcg agcagagcgc gggcgccctg
     1441 gtcgcctacg aggtgacgat cccgcccggc ggcggaccgc cgcacatgca cagccacgag
     1501 gcgttcgagc tcttccgggt cgagtccggc gaactcgcct tctacctcga ggacgacggc
     1561 gagacgcgcc gcagcctggc cgcccccggt gaggtggtcg cgattcccag cggccgcgag
     1621 cacacggtcc gcaacgagtc cgcagaggag gcgcgcgcct tcgcggtcgt ctcccccggt
     1681 gccgagatgg agcgcttcat gcgcagcctc ggggagccgg cctgatggcg acgctgaccg
     1741 ccacccacgc gcccgccgag ctgctcgccc cgctgcagag ctcgtgggcg atgctgctga
     1801 agacccgcaa gcgcgacggc aactgggttg ccacgccggt gaacgtggcg gtcgacggcg
     1861 atcgtgccta cttcgggacg cccgccgaca gcggcaaggt gaagcggctg cgcaacttcg
     1921 acgcggtcga ggtcgcgccc tgcacggtgc gcggcaagcc gaccgggccg ccgctggcga
     1981 tgcgggcgcg gcgactcgag ggagccgagt cggaggcggc gcgcacccgg ctgctcgcca
     2041 agtacccgct cgtgcaccgc ttcctggtcc cgctcgagct gcggctgaaa cgcgtgagcg
     2101 aggtgcgcta cgagctagaa gtcgacgatc tgcaggtcta gcggcagcat gcgggcgcgg
     2161 gtcccggtgg gctgggggtc gatcgcggcg acggcgcctt cggtcagggg caccaggttg
     2221 gtgaccgtgc cgcggacgct gaagcgggtg aagcggtagg gcacgccgtc ggaggcgagc
     2281 ggcgaggggc cgtccatcac gtggaagtgg aggtgggggc cgtcggagtt gccgctgttg
     2341 ccgagccggc cgatcggctg gccggcctgg acgcgctggc cgactttgac catcgcgctg
     2401 cccggttgca ggtgggcgta gaaggcgtag cggccaccgc ccatgtcgac gacgatgtgg
     2461 ttgccgcccg cttcggcggc gccgatgttt ttcgggaagg tgccggccgg cgtctccggg
     2521 atcccgtcga caaccccgac caccttgcct ttgctggccg agtagatcgt cgcgccgaag
     2581 tacgggtagc tggtcagcat gtcgatcggc ccgtcgacca accggttggc cggggtgagc
     2641 tggacgaagt cgatcgcgaa gcgctcggc
//
LOCUS       DASRIG010000243         5742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1940565, whole genome shotgun sequence.
ACCESSION   DASRIG010000243 DASRIG010000000
VERSION     DASRIG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940565"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VFB52_06670"
     CDS             complement(<1..1605)
                     /locus_tag="VFB52_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO06052.1"
                     /translation="MPGFKSVDPKQSFPALEERILARWRETEVFERSLTNRADAEVWS
                     FFEGPPTANGRPGSHHVLSRVFKDVYPRYRTMCGYRVPRKAGWDCHGLPVELEVEKQL
                     GISSKQEIEEFGIAEFNQRCRESVFEYVEEWNRLTERIGFWLDLDDPYVTLEDDYIES
                     VWWSLKRLFDEDRLYEGYKVVPYCPRDGTPLSSHEVAQGYKDVEDPSIYVRFPLLDED
                     AALLVWTTTPWTLPGNVAVAVAPEVAYVRARVDGEVLILAEPLVEKVLGEGTEIVGER
                     FPGSELVGRTYRGPVFELADGGPTGLEGSAAFRVLPGDFVTTEDGTGLVHIAPAFGED
                     DYAVAAANGIFDPTRQETLYNPVRPDGHFDDRVEGFEGRFVKDPEVTRELIADLERRG
                     LLFREQVYEHSYPHCWRCGTPLLYYATSSWYIGTSAVKDQLLANNETIGWHPEHVKHG
                     RFGKWLENNIDWALSRDRYWGTPLPIWRCEDHECGELTAIGSVAELRERATTEVPEGL
                     DLHRPYIDEVAIECGKCGGEMRRVLSV"
     gene            complement(1682..2125)
                     /locus_tag="VFB52_06675"
     CDS             complement(1682..2125)
                     /locus_tag="VFB52_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06053.1"
                     /translation="MQINRTGVFLIGFFGIAGFALCVVPVLLGVPAETALIIASTGFI
                     WFLVAMGLVWYAIRSNKKAERNEWIFKNGIRGTATVLDAGSSATVNEMPLMKLKLELD
                     IPGVAKWKVTKREVMPVFAANRMQEGLVLPAYSNPEDPNEFILVW"
     gene            complement(2126..2722)
                     /locus_tag="VFB52_06680"
     CDS             complement(2126..2722)
                     /locus_tag="VFB52_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06054.1"
                     /translation="MADLETSYQSFAADRQLQMLPAVDVGLLTPLLVESTAGGQLAPA
                     VRGLIADDVEGIVGHLAYKRNKTFRFTVALTEVPSSTAFVPRLFCVRKGRSTRDDEFY
                     GFGARYSKLWTESTKLNERYEVTTGPYQDPNWMRQLFSPALVDYLAHVEPADFSFELA
                     YGYLLGSVEQDDPGADGLAALCEATAKIAKRIAQECEE"
     gene            2808..3683
                     /locus_tag="VFB52_06685"
     CDS             2808..3683
                     /locus_tag="VFB52_06685"
                     /inference="COORDINATES: protein motif:HMM:NF004526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HZO06055.1"
                     /translation="MPAFDLNGKVALVTGGARGIGFETARQLHLRGASVAIVDLDPDQ
                     AREAAERIGERTLGIGADVTDHGAMQAAVAQVVERFGGLDVAMANAGIAQSQVATVRG
                     IGTEEWERVFEVDLLGVWRTVRAALPQIVERQGHIVVVGSVYSFANGVFNSPYAVAKA
                     GVEALGRSLRAELVPLGASAGVAYFGWVDTKLVQDGFDRPHSEKVLSNSPDWLMKRIQ
                     PDEAAAAVVRGIEERAPRIFAPKWWRYVSAFRGLINPLLDRRFDRDKQMIETVRAIEA
                     EADEQGAAAVAAVKR"
     gene            complement(3725..4870)
                     /locus_tag="VFB52_06690"
     CDS             complement(3725..4870)
                     /locus_tag="VFB52_06690"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZO06056.1"
                     /translation="MLRARMGLSGRVRAFTAALVVALVGLTAIAASASALPVRFWGVV
                     PQAIPSEEQFTRLQVGGVKTMRVPFDWGGLQHGGKTTPINFEGIDAIVERAAKHNVEV
                     LPYLTGAPNWAVPSVFVPGDNKAKAPAHLPAAGAAATSWSKFVKEAVARYGSTGSFWA
                     THPAVPKKPIKVWQIWNEPNFKYFVAKPNPAEYGRLVKLSSTAIKSADPSGQLLLAGM
                     FAFPKGCLKEKKPKSPCADDFLDQMYKKTPGVKTKFQWVALHPYTDKYQELTTSIEAF
                     RNTLIKHKDAGKGLWITELGWSSKPVNRATNIFAKGEAGQATQLKGAFTLLRNRATKW
                     KLQRVYWFSVDDSIGTCNFCDGSGLFGDGFVPKKSWYEYVKFAGGTP"
     gene            complement(4875..5396)
                     /locus_tag="VFB52_06695"
     CDS             complement(4875..5396)
                     /locus_tag="VFB52_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06057.1"
                     /translation="MLALLAFACFPVVAQADSVGSVYDPETPEVPKEDKPPKKNTGGS
                     EAEAQKSGGGGTGVTNTPETGDEEESSEVAGGSQGSGNGGSGDGAKTGNGGDTGQSSP
                     GNGSKDVAVGEGSQVPMKHASSTTSDDGSSSPLVPILIAIAALAAISIGAVVVRQRRQ
                     RRGPGGQVSPKAS"
     gene            5378..>5742
                     /locus_tag="VFB52_06700"
     CDS             5378..>5742
                     /locus_tag="VFB52_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06058.1"
                     /translation="MPAEQALTEARRRVGPGYGGGGGAEAFRARLEESAAFLAPLCLI
                     LGIALAGGGFDLGERHVAGLAIWAVVVVLLVLGAAGRAVLERPFFWTSGLIGSVALFS
                     AISSLWSGSIELSVTEADRV"
BASE COUNT          866 a         1913 c         1977 g          986 t
ORIGIN      
        1 caccgagagg acgcggcgca tctcgccgcc gcacttgccg cactcgatcg cgacctcgtc
       61 gatgtagggg cggtgcaggt cgagtccctc ggggacctcg gtcgtcgccc gctcgcgcag
      121 ctcggcgacc gagccgatcg cggtcagctc gccgcactca tggtcctcgc agcgccagat
      181 cggcagcggc gtgccccagt agcggtcgcg gctgagcgcc cagtcgatgt tgttctccag
      241 ccatttgccg aagcgcccgt gcttgacgtg ctcggggtgc cagccgatcg tctcgttgtt
      301 ggcgagcagc tggtccttga ccgcggacgt gccgatgtac cagctcgagg tcgcgtagta
      361 gagcagcggc gtgccacagc gccagcagtg cgggtaggag tgctcgtaga cctgctcgcg
      421 gaagagcagg ccgcggcgct cgaggtcggc gatcagctca cgcgtaacct cggggtcctt
      481 gacgaagcgc ccctcgaatc cctcgacgcg gtcgtcgaaa tgcccgtcgg gccggaccgg
      541 gttgtagagg gtctcctggc gggtcgggtc gaagatgccg ttggcggcgg ccacggcgta
      601 gtcgtcctcg ccgaaggcgg gcgcgatgtg gacgaggccg gtgccgtcct cggtggtgac
      661 gaagtcgccg ggcagcaccc ggaaggcggc tgagccctcg agcccggtcg ggccgccgtc
      721 ggccagctcg aagaccgggc cgcggtaggt gcggccgacc agctcggagc cggggaagcg
      781 ctcgccgacg atctcggtcc cctcccccag caccttctcg accagcggct cagcgaggat
      841 caggacctcg ccgtcgaccc tggcgcgcac gtaggcgacc tcgggcgcga ccgcgaccgc
      901 gacgttgccc ggcagggtcc acggggtcgt cgtccagacg agcagcgcgg cgtcctcgtc
      961 gagcagcggg aagcgcacat atatagaggg gtcctcgacg tctttgtatc cctgcgcgac
     1021 ctcgtgcgag gagagcggcg tgccgtcgcg cgggcagtag ggcacgacct tgtagccctc
     1081 gtagagccgg tcctcgtcga acagccgttt cagcgaccac cagaccgact cgatgtagtc
     1141 gtcctcgagc gtcacgtagg ggtcgtcgag gtcgagccag aagccgatcc gctcggtcag
     1201 ccggttccac tcctcgacgt actcgaagac cgactcgcgg cagcgctggt tgaactcggc
     1261 gatcccgaac tcctcgatct cctgcttgga ggagatcccg agctgctttt cgacctcgag
     1321 ctcgaccggc aggccgtggc agtcccagcc ggccttgcgc ggcacccggt acccgcacat
     1381 cgtgcggtag cgcgggtaga cgtccttgaa cacgcgcgag agcacgtggt gggagccggg
     1441 gcggccattg gcggtcggcg ggccctcgaa aaagctccag acctcggcgt ccgcacggtt
     1501 cgtgagcgag cgctcgaaga cctcggtctc gcgccagcgc gcgaggatcc gctcctcgag
     1561 ggcggggaag gactgtttgg ggtcgacgga cttgaagccg ggcacgaccg ccgattctag
     1621 gaaggagcgg cttgcgggcg ccgccggggc ggctcagcgg gaaacgacgg tcaccgcacg
     1681 atcaccagac gaggatgaac tcgttcgggt cctcggggtt ggaataggcg ggcagaacca
     1741 ggccctcctg catccggttg gcggcgaaga ccggcatcac ttcgcgcttc gtcaccttcc
     1801 acttcgccac cccggggatg tcgagctcga gcttcagctt catcagcggc atctcgttga
     1861 cggtcgcgct gctaccggcg tcgagcacgg tcgcggtgcc cctgatcccg ttcttgaaga
     1921 tccactcgtt gcgctcggcc ttcttgttcg agcggatcgc gtaccagacc agtcccatcg
     1981 cgaccaggaa ccagatgaac ccggtcgagg cgatgatcag cgcggtctcg gcgggaacgc
     2041 cgagcagcac cgggacgacg cagagggcga agccggcgat cccgaagaag ccgatcagga
     2101 agacgccggt gcggttgatc tgcatctact cctcacactc ctgcgcgatc cgcttggcga
     2161 ttttggcggt ggcctcgcag agcgccgcca ggccgtcggc gccggggtcg tcctgctcga
     2221 cgctgccgag caggtagccg taggcgagct cgaaggagaa gtcggccggc tcgacgtggg
     2281 cgaggtagtc gaccagcgcg ggcgagaaca gctgccgcat ccagttcggg tcctggtagg
     2341 gaccggtggt gacctcgtag cgctcgttca gcttcgtgct ctcggtccag agcttcgagt
     2401 agcgggcgcc gaagccgtag aactcgtcgt cgcgggtgct gcgccccttg cgcacgcaga
     2461 acagccgcgg cacgaacgcg gtcgagctcg gcacctcggt gagcgcgacg gtgaagcgga
     2521 aggtcttgtt gcgcttgtag gcgaggtggc cgacgatccc ttcgacgtcg tcggcgatca
     2581 ggccgcgcac ggcgggcgct agctggcccc cggccgtcga ctcgacgagc aggggcgtca
     2641 gcaggccgac gtccacggcg ggcagcatct ggagctggcg atcggcggcg aaggactggt
     2701 aggaggtctc gaggtcggcc aagaccccgg cagcctaccc acgatccagg cgctaacagg
     2761 gaccgcgaca cgtttccgtg acgcggtctc gggtacgctc cccggcgatg ccggccttcg
     2821 acctcaacgg caaagtcgcg cttgtcaccg gcggcgcccg cgggatcggc ttcgagaccg
     2881 cgcgccagct gcacctgcgc ggtgcctcgg tcgcgatcgt cgacctcgac cccgaccagg
     2941 cccgcgaggc ggccgagcgg atcggcgagc gcacgctcgg gatcggcgcc gacgtcaccg
     3001 accacggcgc gatgcaggcc gccgtcgccc aggtggtcga gcgcttcggc ggcctcgacg
     3061 tggcgatggc caacgccggc atcgcccagt cccaggtcgc gaccgtgcgc gggatcggca
     3121 ccgaggagtg ggagcgcgtc ttcgaggtcg acctgctcgg cgtctggcgc acggtgcgcg
     3181 cagcgctgcc gcagatcgtc gagcgccagg gccacatcgt cgtcgtcggc tccgtctact
     3241 ccttcgccaa cggcgtcttc aacagcccct acgcggtcgc caaggccggg gtcgaggcgc
     3301 tcgggcgctc gctgcgcgcg gagctggtcc cgctcggcgc cagcgccggc gtcgcctact
     3361 tcggctgggt cgacaccaag ctcgtccagg acggcttcga ccgcccccac tccgagaagg
     3421 tgctgtccaa ctcgccggac tggctgatga agcgaatcca gcccgacgaa gccgcggccg
     3481 ccgtggtccg cgggatcgag gaacgggcgc cgcgcatctt cgcccccaag tggtggcgct
     3541 acgtctccgc cttccgcggc ctgatcaacc cgctgctcga ccgccgcttc gaccgcgaca
     3601 agcagatgat cgaaacggtt cgcgcgatcg aggccgaggc cgacgagcag ggcgccgccg
     3661 ccgtcgcggc ggtcaagcgc taggagtcct tcctaactgc tcgtcgaagc cccgtccggc
     3721 ggcgctacgg ggtgccgccg gcgaacttca cgtactcgta ccaggacttc ttcggcacga
     3781 agccgtcacc gaagaggccg gagccgtcgc agaagttgca ggtcccgatc gagtcgtcga
     3841 ccgagaacca gtagacgcgc tgcagcttcc atttcgtcgc tctgttgcgc agcagggtga
     3901 aggcgccctt gagctgggtc gcctgccccg cttcgccttt tgcgaagatg ttggtcgccc
     3961 ggttgaccgg cttcgaggac cagccgagct cggtgatcca gagccccttg ccggcgtctt
     4021 tgtgtttgat caaggtgtta cggaacgctt cgatcgaggt ggtcagttcc tgatatttgt
     4081 ccgtgtaggg gtgcagtgca acccactgga acttggtttt gacccccggc gtctttttgt
     4141 acatctggtc gaggaagtcg tccgcgcagg ggcttttcgg cttcttttct ttcaggcagc
     4201 ccttggggaa cgcgaacata cccgcgagca gcagctggcc gctcgggtcg gccgatttga
     4261 tcgccgtgct cgagagcttg accaggcggc cgtactcggc tgggttgggc ttcgcgacga
     4321 agtacttgaa gttcggctcg ttccagatct gccagacctt gatcggcttc ttcggcacgg
     4381 ccgggtgggt cgcccagaag ctgccggtgc tgccgtagcg ggcaaccgct tctttgacga
     4441 acttcgacca cgaggtcgcc gccgcgcccg cggccggcag gtgggccggg gctttcgctt
     4501 tgttatcgcc gggaacgaag acgctgggca cggcccagtt cggcgctccg gtcaggtacg
     4561 gcaacacctc gacgttgtgc ttggccgccc gttcgacgat cgcgtcgatg ccctcgaagt
     4621 tgatcggggt ggttttgccg ccgtgctgca ggccgcccca gtcgaacggg acccgcatcg
     4681 tcttcacgcc gcccacctgc aggcgggtga actgttcttc gctcgggatc gcctggggca
     4741 cgacgcccca gaagcgcacc ggcagcgccg aggcgctcgc cgcgatcgcg gtcaagccga
     4801 ccagggcgac caccagcgcc gccgtaaagg cacgcacacg accgctgagt cccatcctcg
     4861 ctctcaacat cgggctagct cgccttgggc gagacctggc caccaggacc gcggcgctgg
     4921 cgccgctgcc ggaccacgac cgcgccgatc gagatcgcgg caagcgcggc gatcgcgatc
     4981 aggatcggga ccaggggcga agaggagccg tcgtcggagg tggtgctgga cgcgtgcttc
     5041 attgggacct gactcccctc acccacggcg acgtccttgg agccgttgcc tggactactc
     5101 tggccggtgt ctccaccgtt tccagtcttc gcgccatcgc cgctgccgcc gttgccggaa
     5161 ccttgactgc caccagctac ttcagaactt tcttcttcat ccccggtttc aggggtattg
     5221 gtgacgccgg ttccaccgcc gccggacttt tgggcttcgg cttcggagcc gccggtgttc
     5281 ttcttcggcg gcttgtcttc cttcggaact tcgggagttt cgggatcgta gacggagcca
     5341 accgaatcag cttgagcgac caccgggaaa caggcaaatg ccagcagagc aagcactgac
     5401 agaagcaaga aggcgggtcg gacctggcta cggtggcggt ggcggggccg aggcattccg
     5461 cgcaaggtta gaggaatcgg cggcgttcct cgctccgctc tgcctgattc tcggcattgc
     5521 ccttgctggt gggggattcg acctgggcga acgtcatgtc gccggcctgg cgatctgggc
     5581 agtcgtagtt gtcctgctgg tccttggggc agctggccga gcggtgctcg agcgcccctt
     5641 cttctggacg agtgggctga tcggttctgt ggcgcttttt tctgcaatct catctctctg
     5701 gtccggttcg atcgagttga gcgtcaccga ggcagatcgc gt
//
LOCUS       DASRIG010000244         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1682454, whole genome shotgun sequence.
ACCESSION   DASRIG010000244 DASRIG010000000
VERSION     DASRIG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682454"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFB52_06705"
     CDS             <1..848
                     /locus_tag="VFB52_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZO06059.1"
                     /translation="YNSPLSEIACLGFEYGYSAATPSALVLWEAQFGDFANAAQVIID
                     SFIVSGEAKWGQTSRLTLLLPHGYEGSGPEHSSARIERFLALGAEGNIRIANPTTAAQ
                     YFHLLRRQALIRKPRPLIVFTPKGLLRLDRATSKVEDLTGGEFLYTLDDPTAEGRREK
                     VERLVLCTGKIYYDIDASERRAEAENVAIVRVEMLYPFAKEQLQRIIGEYPNLKEIAW
                     VQEEPRNMGAWKVMSRRFPEVLPEGVELKYIGRPGRASPGEGYSGAHSREQERIVLTA
                     LTPGT"
     gene            855..1187
                     /locus_tag="VFB52_06710"
     CDS             855..1187
                     /locus_tag="VFB52_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06060.1"
                     /translation="MAGRPLQKPVPGKLFVWVKRLSWIELVVFAGLLFFWLAPGFETE
                     TMLFGWAHGLGFIALCIVIWVACVRRESPYTLLAATLTPVGPVGSVIGIELIERKGWG
                     IAAPTHGG"
     gene            complement(1428..4241)
                     /locus_tag="VFB52_06715"
     CDS             complement(1428..4241)
                     /locus_tag="VFB52_06715"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZO06061.1"
                     /translation="MSSDSFGARSTLEVSGREYEVFRLDALQERFDVARLPYSIKVLL
                     ENVLRLEDGVSVSAADVEAIASWDAAAEPSVEIPFQPARVLMQDFTGVPAVVDLAAMR
                     DAMEEIGGDPSAINPLVDVDLVIDHSVQVDAFGNERAFAVNAARDFERNLERYSFLKW
                     GQQSFDNFRVVPPATGICHQVNLEYLAQVVYSRENGGVLQAYPDTLVGTDSHTTMVNG
                     LAVLGWGVGGIEAEAAMLGQPISMLLPQVVGFKLDGTLPEGATATDLVLTVTEMLRER
                     GVVSKFVEFFGPGLAKLPLADRATLGNMSPEFGSTCAIFPVDAETLRYMDLTGRPTRT
                     IELVDAYAREQGMFVDESSPDPVFSDLLELDLGAVVPSLAGPKRPQDRVPLSEAKESF
                     LEAMGEFEPEAAEQMGNGRDEAIEESFPASDPPAEDHDGEAGKPRPAVTGASVAAAEQ
                     RMTDDAIGVTLIEDGSEFELDHGRVVIAAITSCTNTSNPSVMIGAGLLARNAVEAGLT
                     RKPWVKTSLAPGSTVVTDYLDRAGLTEYLNALQFNLVGYGCTTCIGNSGPLAEEISAA
                     VAEKDLAVVSVLSGNRNFEGRINQDVKANYLASPPLVVAYALAGRMDIDLATEPLGEG
                     SDGQPVFLKDVWPDASEIKEVVGNSVASDMFSDNYASVFSGDDEWNAVEVPAGDRYTW
                     PDSTYVRRPSFFEAMPADPPGVSPIAGARVLAKLGDSITTDHISPAGAIKRDSPAGKW
                     LMDQGVEPGDFNSYGARRGNHEVMIRGTFANIRLRNQLVEKAGGHTRHFPDGEEAFIY
                     DAAVKYAEEGVPLIVLAGKEYGSGSSRDWAAKGTKLLGVRAVIAESFERIHRSNLIGM
                     GVLPLQFADGESIESLGLTGAESFDFGDLEDGHAKSVAVTATSDDGASTEFTATVRLD
                     TPNEITYFQNGGILQTVLRKLNA"
     gene            complement(4271..5074)
                     /locus_tag="VFB52_06720"
     CDS             complement(4271..5074)
                     /locus_tag="VFB52_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06062.1"
                     /translation="MDLSGRTALLTGATGGLGRAIARSLSGRGAKLLLSARKQDALEA
                     LAAELPGEGHRALPADLAEEGAAEQLAAAAAGTEILVANAGLPAAGRMEDFTPEQVKR
                     ALRVNLESPMLLAQALYPAMLERRSGHLVFISSLAGKAPAPRSSIYNATKFGLRGFAL
                     GLRTDIRPQGVGVSLVSPGFIREAGMFAESGASSPAGLGTATPEQVAEATVKAIVSDK
                     VEIAVAPPQQRFLAHFGLANPGIAVRVSSGSAGQKSAAEIADGHSKDKR"
     regulatory      5199..5349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5352..5909
                     /locus_tag="VFB52_06725"
     CDS             5352..5909
                     /locus_tag="VFB52_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06063.1"
                     /translation="MRHNLKLKLGIAAIAILSVLAIAGPTSASAKCTGGGVSPAEIDV
                     CTPTAKAKLLDNGTLVPPKSAPPRVKAVIAAANKISRKPYIYGGGHARWWDKGYDCSG
                     AVSYALRGGKFLTSPLPSTGLEEWGIAGEGRWITVYSNPGHAYAVIAGYRWDTSGNSG
                     DETGPRWHEDLRESTGFVARHPAGY"
BASE COUNT          941 a         2082 c         2231 g          964 t
ORIGIN      
        1 tctacaacag tccgctctcg gagattgcct gcctcgggtt cgagtacggc tactcggcgg
       61 cgaccccaag cgccctggtc ctctgggagg cccagttcgg cgacttcgcc aacgcggccc
      121 aggtgatcat cgacagcttc atcgtctccg gcgaggcgaa gtggggccag accagccgcc
      181 tgacgctgct gctgccgcac ggctacgagg gctccggccc cgagcactcc agcgcccgga
      241 tcgagcgctt cctcgcgctc ggcgccgagg gcaacatccg gatcgccaac ccgaccacgg
      301 cggcccagta cttccacctg ctgcgccgcc aggcgctgat ccgcaagccg cggccgctga
      361 tcgtgttcac gccgaagggg ctgctgcgcc tcgaccgcgc aacctcgaag gtcgaggacc
      421 tgaccggcgg cgagttcctc tacacgctcg acgacccgac cgccgagggg cggcgcgaga
      481 aggtcgagcg gctggtcctc tgcaccggca agatctacta cgacatcgac gcctccgagc
      541 gccgcgccga ggccgagaac gtggcgatcg tgcgggtcga gatgctctac ccgttcgcca
      601 aggaacagct gcagcggatc atcggcgaat acccgaacct gaaggagatc gcctgggtcc
      661 aggaggagcc gcgcaacatg ggcgcctgga aggtgatgtc gcggcgcttc cccgaggtgc
      721 tgcccgaggg cgtcgagctc aagtacatcg gccgtcccgg ccgcgccagc ccgggcgagg
      781 gctactccgg ggcgcactcg cgcgagcagg aacggatcgt gctcaccgcg ctcacccccg
      841 gaacctaggc cgcgatggcg gggcgcccgt tgcagaagcc cgtgccgggc aagctgttcg
      901 tctgggtcaa gcggctctcc tggatcgagc tggtggtctt cgccgggctg ctgttcttct
      961 ggctggcccc ggggttcgaa accgagacga tgctcttcgg ctgggcccac gggctcggtt
     1021 tcatcgcact ttgcatcgtg atctgggtcg cctgcgtgcg ccgcgagtcc ccctacaccc
     1081 tgctcgccgc gaccctcacc ccggtcggcc cggtcggcag cgtgatcggg atcgagctga
     1141 tcgaacgcaa gggctggggc atcgccgccc cgacgcacgg ggggtaaggc ccccaaccgg
     1201 cgtccgcacc gtgcacccga aggttcctaa agcgctcggg tgtgcttgac cggcggcatt
     1261 tcaggcgcta tagctacttg acgactgttc ctcagggcag tcccgtccgg gggaacggcg
     1321 aggcgtcggc atccctccct ggctaccgac gcactggtgc agtgagggtc gcgcggaccc
     1381 ccctgcctcc cgcggcccac ctgcaccctg cccgaaaccg gagggtgtca ggcgttgagc
     1441 ttgcgcagaa cggtctgcag gatgccgccg ttctggaagt aggtgatctc gttgggggtg
     1501 tcgaggcgga cggtggcggt gaactcggtg ctggcgccgt cgtcgctggt ggcggtcacg
     1561 gcgacggatt tggcgtggcc gtcctcgagg tcgccgaagt cgaaggactc ggcgccggtc
     1621 aggccgaggg actcgatgct ctcgccgtcg gcgaactgaa gggggagcac gcccatcccg
     1681 atcaggttcg agcggtggat gcgctcgaag gactcggcga tcacggcgcg gacgccgagc
     1741 agcttggtcc ccttcgccgc ccagtcgcgg gaggagccgg agccgtactc cttgccggcc
     1801 agcacgatca gcggcacgcc ttcctcggcg tacttgactg cggcgtcgta gatgaaggct
     1861 tcctcgccgt cggggaagtg gcgggtgtgg ccgccggcct tctcgaccag ctggttgcgc
     1921 aggcggatgt tggcgaaggt gccgcggatc atcacctcgt ggttgccgcg gcgggcgccg
     1981 taggagttga agtcgccggg ttcgacgccc tggtccatca gccacttgcc ggccgggctg
     2041 tcgcgtttga tcgcgccggc gggcgagatg tggtcggtgg tgatcgagtc gccgagcttg
     2101 gcgagcacgc gggcgccggc gatcggcgag accccgggcg ggtcggcggg catcgcctcg
     2161 aagaagctcg gccggcgcac gtaggtcgag tcgggccagg tgtagcggtc gcccgcgggc
     2221 acctcgaccg cgttccattc gtcgtcgccg ctgaacacgc tggcgtagtt gtcggagaac
     2281 atgtcggagg cgaccgagtt gccgacgacc tccttgatct cgctggcgtc gggccagacg
     2341 tccttgagga agaccggctg gccatcggag ccctcgccga gcggttcggt ggcgaggtcg
     2401 atgtccatcc ggccggcgag cgcgtaggcg accaccagcg gcgggctggc caggtagttg
     2461 gccttcacgt cctggttgat ccggccctcg aagttgcggt tgccggagag caccgagacg
     2521 accgcgaggt ctttctcggc gaccgcggcg gagatctcct cggccagcgg gccggagttg
     2581 ccgatgcagg tggtgcagcc gtagccgacc aggttgaact ggagcgcgtt caggtactcg
     2641 gtcagcccgg cccggtcgag gtagtcggtg accacggtcg agcccggcgc cagcgaggtc
     2701 ttcacccagg gcttgcgggt gagcccggct tcgaccgcgt tgcgggcgag cagcccggcg
     2761 ccgatcatca cgctcgggtt cgaggtgttg gtgcagctgg tgatcgcggc gatcaccacg
     2821 cggccgtggt cgagttcgaa ttcggagccg tcctcgatca gggtgacccc gatcgcgtcg
     2881 tcggtcatcc gctgctcggc cgcggcaacc gaggcaccgg tgaccgcggg gcgcggtttg
     2941 ccggcctcgc cgtcgtggtc ctcggcgggc gggtcggagg ccgggaagga ctcttcgatc
     3001 gcctcgtcgc ggccgttgcc catctgctcg gcggcctcgg gttcgaactc gcccatcgcc
     3061 tcgaggaagg actccttggc ctcggacagc ggcacccggt cctgtggccg tttcgggccg
     3121 gcgaggctgg ggacgacggc gccgaggtcg agctccagca ggtccgagaa gaccgggtcg
     3181 gggctcgact cgtcgacgaa catgccctgc tcgcgcgcgt aggcgtcgac cagctcgatc
     3241 gtccgggtcg gacggccggt caggtccatg tacctgaggg tctcggcgtc gaccgggaag
     3301 atcgcgcagg tcgagccaaa ctccggcgac atgttgccga gcgtggcgcg gtcggccagc
     3361 ggcagcttcg ccaggccggg gccgaagaac tcgacgaact tggagacgac gccgcgctcg
     3421 cgcagcatct cggtcacggt gaggacgagg tcggtcgcgg tcgcgccctc gggcagcgtc
     3481 ccgtcgagct tgaagccgac cacctgcggc agcagcatcg agatcggctg gcccagcatc
     3541 gccgcctcgg cctcgatccc gccgaccccc cagccgagca cggcgaggcc gttgaccatc
     3601 gtcgtgtgcg agtcggtgcc gaccagggtg tccggatagg cctgcagcac gccgccgttc
     3661 tcgcggctgt agacgacctg ggcgaggtac tcgaggttga cctggtggca gatcccggtc
     3721 gccggcggga ccacgcggaa gttgtcgaag gactgctggc cccatttgag gaacgagtag
     3781 cgctcgaggt tgcgctcgaa gtcgcgggcg gcgttgacgg cgaaggcgcg ctcgttgccg
     3841 aaggcgtcga cctggaccga gtggtcgatc accaggtcga cgtcgaccag cgggttgatc
     3901 gcgctcgggt cgccgccgat ctcctccatc gcgtcgcgca tcgcggcgag gtcgacgacg
     3961 gcgggaacgc cggtgaagtc ctgcatcagc acgcgggcgg gctggaacgg aatctcgacg
     4021 ctcggttcgg cggccgcgtc ccagctggcg atcgcctcga cgtcggcggc gctgacggac
     4081 accccgtcct cgagccgcag cacgttctcg agcaggacct tgatcgagta gggcaggcgg
     4141 gcgacgtcga agcgctcttg gagggcgtcg agccggaata cctcgtactc gcgcccgctc
     4201 acctcaaggg tcgaacgggc gccaaaagaa tcgctgctca tattggggga gttccttcca
     4261 actggggggt ttacctcttg tccttcgagt gtccgtcggc gatctcggcg gccgacttct
     4321 ggccggccga gccgctcgag acgcgcaccg cgatgccggg gttggcaagt ccgaagtggg
     4381 cgaggaagcg ctgctggggc ggcgccaccg cgatctcgac cttgtccgag acgatggcct
     4441 tgaccgtcgc ctcggcgacc tgctcggggg tggcggtgcc gaggcccgcc ggtgagctcg
     4501 cccccgactc ggcgaacatg cccgcctcgc ggatgaatcc gggcgagacg agcgagacgc
     4561 cgacgccctg cgggcggatg tcggtgcgca gcccgagcgc gaagccgcgc aggccgaact
     4621 tggtcgcgtt gtagatcgaa gagcgggggg ccggcgcctt gccggccagc gaggagatga
     4681 agacgaggtg gccggagcgg cgctcgagca tcgccgggta gagcgcctgc gcgagcagca
     4741 tcggcgactc gagattgacc cgcagggcgc gcttgacctg ctcgggcgtg aagtcctcca
     4801 tccgcccggc ggccggcagg ccggcgttgg cgacgaggat ctcggtcccc gcggcggctg
     4861 cggcgagctg ctcggcggca ccctcctcgg cgaggtcggc gggcagcgcc cggtggccct
     4921 cgcccggcag ttcggcggcc agcgcttcca gcgcgtcctg cttgcgcgcg ctgagcagca
     4981 gcttggcgcc gcgcccggag agcgagcgcg cgatcgcgcg gccgagcccg ccagtggcgc
     5041 cggtgaggag tgcagtgcga ccggaaagat ccatcccacc gggcaagcta ccagccgtgc
     5101 ggccgacagg aatctgggat gcaccctcga aattccaggg aatgtttgcg ttttgtgagc
     5161 agagcgccgg acgatggtgt agattgccgc gagtccgaaa gaccggaacg ccgaatcccc
     5221 aatgccaaaa gcgcggcggg gagcggggga accacgtttt cggggctaac tcaccgctat
     5281 aggtgggggc gcgagcttcc ttcagcgcca gcccgacagc taaccccgta ggccgacgaa
     5341 ggagaatcgg tttgcgccac aacttgaagc tgaagctcgg gatcgcggct atcgcgattc
     5401 tctcggtgct cgccatcgcg ggcccgacga gcgcctcggc caagtgcacc ggcggcggtg
     5461 tctcgccggc cgaaatcgac gtctgcaccc cgacggcgaa agcgaagctg ctcgacaacg
     5521 gcaccctggt accgccgaag agcgccccgc cccgggtcaa ggccgtgatc gccgcggcca
     5581 acaagatcag ccgcaagccc tacatctacg gcggcggcca cgcccgctgg tgggacaaag
     5641 gctacgactg ctccggcgct gtctcctacg ccctccgcgg cggcaaattc ctcacctccc
     5701 cgctgccctc cacgggcctc gaagagtggg gcattgccgg cgaaggccgc tggatcaccg
     5761 tctactcgaa ccccggccac gcctacgccg tgatcgccgg ctaccgctgg gacacctcgg
     5821 gtaactccgg cgacgaaacg ggtccgcggt ggcatgaaga cctgcgcgag agcaccggat
     5881 tcgtcgcgcg gcaccccgct ggctactaag gctggctagg aggggtcggg tacccctctc
     5941 cacttaactt ttttgggaat cgagcggcgg ctacacgcct cgattcgctg aagcaaaaaa
     6001 gtacaagacg ttccgagggg tacccgaccc ctccccacca accgcagcaa tcttccgggt
     6061 gccgcgggac gaggcagcgg cgaagtcgcg cgggagcatg ccgcaacgga cttagctgcc
     6121 gccaggcaat gctctcaagg tgctgtcgcc gcggcgctag acgcgtcgtg gtctcgcgga
     6181 tttccgagac ggcttgagcg gagagggtgg ggtgcctc
//
LOCUS       DASRIG010000245         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2991482, whole genome shotgun sequence.
ACCESSION   DASRIG010000245 DASRIG010000000
VERSION     DASRIG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991482"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFB52_06730"
     CDS             <1..1011
                     /locus_tag="VFB52_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZO06064.1"
                     /translation="TMATVIASQAVISGAFSVAHQAVRLGYLPRLRIVHTSAETEGQI
                     YVPWINWLLLVSVLTLVFAFQTSAALAFAFGMAVTATINITALLFLYVARHRWGWPLW
                     WVVGLGVLMLSVDLLFFAANLTKIFHGAWLPLLIGVVAFTVMTTWKRGRELVTERRER
                     KEGSLREFVDALHSREPPLPRTPGTAVFLNRNVETTPLAMRANVEHNRILHEHVVVLS
                     IETLPVPEVPDSARIVIDDLGYADDGISHATARFGYIEQPDVPAALLLAPAAGLECPL
                     EIDEATYFLSTIDLQTTDAPGMNRYRKRLFVAASHITADAAEYFQLPRERTVIMGSVI
                     EV"
     gene            1036..2196
                     /locus_tag="VFB52_06735"
     CDS             1036..2196
                     /locus_tag="VFB52_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO06065.1"
                     /translation="MSGISSSFRLGGEPPPFGWGLLALATVVAMATGLVYALADVAPV
                     VSLGVVYLPGVLLISTFWGWRLGLATAVASALAFNFFHIPPTGRFTIAADRDGVALVV
                     FAIVAVASSALAELARARAAEAERRREEADRLLTELRAAEDERDRMEAEAIEAAALRR
                     SDELKTALLRSVSHDLRTPLTSIIAAGAALDSPSATAEERHELSEAVVAEGQRLSRLV
                     ENLIDVSRLEAGDTEPRREPVDLAGLIEAARASVGAGPEDVRLALDAELPALEGDAAQ
                     LERAFANLLENAVRHGGGRPVLVRSRTSGGRLVVRVVDQGPGIPEPERARLFEPFYRR
                     EGGPGGGFGLGLAIAKGFVEANGGEIAVESMPGQGSTFVVSFPERVGEGGAA"
     gene            2193..>2442
                     /locus_tag="VFB52_06740"
     CDS             2193..>2442
                     /locus_tag="VFB52_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO06066.1"
                     /translation="MSELPRILVCDDEPQILRALRVILRDAGYEAVTASTGEEALDRA
                     AVKPPQAAILDLMLPDIDGVEVTRRLREWSEMPILVLSA"
BASE COUNT          311 a          872 c          902 g          357 t
ORIGIN      
        1 acgatggcga ccgtgatcgc ctcgcaggcg gtgatcagcg gcgccttctc ggtcgcccac
       61 caggcggtcc ggctcggcta cctgccgcgg ctgcggatcg tccacacctc ggccgagacc
      121 gagggccaga tctacgtgcc ctggatcaac tggctgctgc tggtctcggt gctgaccctg
      181 gtcttcgcct tccagacctc ggccgcgctc gccttcgcct tcgggatggc ggtgacggcg
      241 acgatcaaca tcaccgcgct gctcttcctc tacgtcgccc gccaccgctg gggctggccg
      301 ctgtggtggg tggtcggtct cggcgtgctg atgctgagcg tcgacctctt gttcttcgcg
      361 gccaacctga ccaagatctt ccacggcgcc tggctgccgc tcttgatcgg ggtggtcgcc
      421 ttcaccgtga tgacgacctg gaaacgcggc cgcgaactgg tcaccgagcg gcgcgaacgc
      481 aaggagggct cgctgcgcga attcgtcgac gccctgcaca gccgcgagcc gccgctgccg
      541 cgcacccccg gcaccgccgt cttcctcaac cgcaacgtgg agacgacgcc gctggcgatg
      601 cgcgccaacg tcgagcacaa ccggatcctg cacgagcacg tcgtcgtgct ctcgatcgag
      661 accctgccgg tgcccgaggt gccggactcc gcgcggatcg tgatcgacga cctcggctac
      721 gccgacgacg ggatcagcca cgcgaccgcg cgcttcggct acatcgaaca gcccgacgtg
      781 ccggcggccc tgctgctcgc cccggcggcc gggctcgagt gcccgctgga gatcgacgag
      841 gcgacctact tcctctcgac catcgacctc cagaccaccg acgcgccggg aatgaaccgc
      901 taccgcaagc gcctcttcgt cgccgcctcc cacatcaccg ccgacgccgc cgagtatttc
      961 cagctgccgc gcgagcgcac cgtgatcatg ggctcggtga tcgaggtcta agcccccggg
     1021 tctaggctgc ggtcgatgag cgggatcagc tccagctttc gcctcggcgg cgagccgccg
     1081 ccgttcggct ggggtctatt ggcgctcgcc acggtggtcg cgatggcgac ggggctggtc
     1141 tacgcgctcg ccgacgtcgc gccggtggtc tcgctcgggg tcgtctatct gcccggcgtg
     1201 ctgctgatct cgaccttctg gggctggcgg ctggggctgg cgaccgcggt ggcgagcgcg
     1261 ctcgccttca acttcttcca catcccgccg accgggcgct tcacgatcgc cgccgaccgc
     1321 gacggggtgg cgctggtcgt gttcgcgatc gtcgccgtcg ccagcagcgc cctggccgag
     1381 ctggcgcggg cgcgggcggc ggaggccgag cggcgccggg aggaggcgga ccggctgctt
     1441 accgagctgc gcgccgccga ggacgagcgt gaccggatgg aggcggaggc gatcgaggcg
     1501 gcggcgctgc gccgcagcga cgagctgaag acggcgctgc tgcgctcggt ctcgcacgac
     1561 ctgcgcacgc cgctgacctc gatcatcgcc gccggcgccg cgctcgactc gccgagtgcg
     1621 accgcggagg agcgccacga gctgagcgag gccgtggtcg ccgaagggca gcggctctcg
     1681 cgcctggtcg agaacctgat cgacgtctcg cggctggagg cgggcgacac cgagccgcgc
     1741 cgcgaaccgg tcgacctcgc cggcctgatc gaggcggcgc gggcctcggt cggtgcgggg
     1801 ccggaggacg tgcggctggc gctcgacgcg gagctgccgg cgctcgaagg cgacgccgcc
     1861 cagctcgagc gcgccttcgc caacctgctc gagaacgcgg tccgccacgg cggcggccgg
     1921 ccggtgctgg tgcgctcgcg gaccagcggc gggcggctgg tcgtgcgcgt ggtcgaccaa
     1981 gggccgggaa tcccggagcc cgagcgggcg cgcctgttcg agccctttta caggcgcgag
     2041 ggcggccccg gcggcggctt cgggctcggc ctggcgatcg ccaaagggtt cgtcgaggcc
     2101 aacggcggcg agatcgcggt cgagtcgatg cccggccagg gcagcacctt cgtggtcagc
     2161 ttcccggagc gggtggggga ggggggcgcc gcgtgagcga gctgccgcgg atcctcgtct
     2221 gcgacgacga gccgcagatc ctgcgggcgc tgcgggtgat cctgcgcgac gccggctacg
     2281 aggcggtgac cgcgagcacc ggggaggagg cgctcgaccg cgccgcggtc aaaccgccgc
     2341 aggcggcgat cctcgacctg atgctgccgg acatcgacgg ggtggaggtg acccggcggc
     2401 tgcgcgagtg gagcgagatg ccgatcctgg tgctctcggc gg
//
LOCUS       DASRIG010000246         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_899512, whole genome shotgun sequence.
ACCESSION   DASRIG010000246 DASRIG010000000
VERSION     DASRIG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899512"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFB52_06745"
     CDS             complement(<1..807)
                     /locus_tag="VFB52_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HZO06067.1"
                     /translation="MRSPFAYTPRPGPLQAASPGAAVVYLGALVAVAFLYSNPLVLLA
                     AGAGATIAGLLAGARGAVWVALKMGFALAVLIVVVNALVVDRGATVLARLGDWPWLGE
                     VDVTFEAIVAGLSIGLRAAVAMVAFAVYSACVDPDRVLRALRPIAGRSALTATLVSRL
                     VPVAAADAARLRDARRLRGPGAAEVGKAALARRLLAGSLDRAVDVAATLELRGYSLDA
                     AHRRSRRNALPGLWGPERPKSRKGSRYDLRFYLAGALVLAAAIAGKVMGAD"
     gene            complement(804..1742)
                     /locus_tag="VFB52_06750"
     CDS             complement(804..1742)
                     /locus_tag="VFB52_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HZO06068.1"
                     /translation="MIRSRGTAVAAALLLAALAAAGCGLGPGEEVGSVELTVTREFGS
                     QPMVRSSVEVTESDTVMRVLEGEAEIETRYGGGFVKSIEGYTEGRRDGHPYDWFFFVN
                     GVESPIGAADVDLQGGERIWWDYRDWEATNRVPAVVGSWPAPFTTGYEGRAHPVAVVC
                     AGGGEACTDVEAALEDEGVKLASGTPDGAIRVLVGPWSQVRTDPAAALIEDGPEESGV
                     YADFERQAGGVGSAGAFSLVALDVNGDPASDLGPDAGLVAATRRYEAPPVWVVTGGTP
                     AAVQAAAELLEPKLLRDYFAVAIAGQLIYPLPPEHP"
     gene            complement(1742..2437)
                     /locus_tag="VFB52_06755"
     CDS             complement(1742..2437)
                     /locus_tag="VFB52_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06069.1"
                     /translation="MSVSVAAAKGFSADLRVVGKGGKILAEKTVTTGAISVKTSPKAT
                     CFGPGSGGSGDSVALKENNALALLARGAKSTPALNPLLISDSFDFGLALCGVGSGVAK
                     GGLSWYLKVNHKGTMVSADTTKIKAGDEVLFALAESDPKTFAYPEELALVVPAKAQAG
                     KAFTVTAYSYDEKGKRKPAAGVKVTGASGLTSATGKATVTLSGPKRLLATKGDLIPDR
                     AAVCVGGKCPAGS"
BASE COUNT          366 a          936 c          938 g          359 t
ORIGIN      
        1 gtcggctccc atcaccttgc ccgcgatcgc cgccgcgagc acgagcgctc ccgccaagta
       61 gaagcgcagg tcgtaacgcg agcccttccg gcttttcggg cgctcgggcc cccacaaacc
      121 cggaagcgcg ttccgccgcg agcgccggtg cgctgcgtcg aggctgtagc cgcgcagctc
      181 cagggtcgcg gccacgtcga cggcgcggtc gagggagccg gcgagcaggc gacgggcgag
      241 agcggccttg ccgacctcgg cggcgccggg gccgcgcagg cggcgagcgt cgcgcaggcg
      301 ggcggcgtcg gcggcggcaa ctgggaccag acgggagacc agggtcgcgg tcagagccga
      361 gcggccggcg atcgggcgca gggcgcgcag cacccggtcg gggtcgacgc aggccgagta
      421 gacggcgaag gcgaccatcg cgaccgccgc ccgcagcccg atcgagaggc cggcgacgat
      481 cgcctcgaag gtgacgtcga cctcgccgag ccagggccag tcgccgagcc gcgccagcac
      541 ggtcgcgccg cggtcgacga cgagggcgtt gacgacgacg atcagcaccg ccagcgcaaa
      601 gcccatcttc agcgccaccc aaaccgcgcc gcgggcgccg gcgagcaggc cggcgatcgt
      661 cgcccccgca ccggccgcga gcaggaccag cgggttcgag tagaggaagg cgacggcgac
      721 gagcgcgccg aggtagacga ccgcagctcc cggcgatgcc gcctgcagcg gccccggcct
      781 cggcgtgtag gcgaacggcg acctcatgga tgttctggcg gcagcgggta gatgagctgc
      841 cccgcgatcg ccaccgcgaa atagtcgcgc agcagcttcg gttcgagcaa ctcggcggcc
      901 gcctgcaccg cggctggcgt gcccccggtg acgacccaga ccggcggcgc ttcgtagcgc
      961 cgggtggcgg cgacgaggcc ggcgtcgggg ccaaggtcgc tggcgggatc gccgttgacg
     1021 tcgagcgcga ccaggctgaa ggcgccggcg ctccccaccc caccggcctg ccgttcgaag
     1081 tcggcgtaga cgccgctttc ttccggcccg tcctcgatca gcgccgccgc cgggtcggtc
     1141 cgcacctgcg accaggggcc gacgaggacg cggatcgcac cgtcgggggt gccggaggcg
     1201 agcttgacgc cttcgtcctc gagcgccgct tccacgtcgg tgcacgcctc tcccccgccc
     1261 gcgcagacga cggcgaccgg gtgggccctg ccttcgtagc cggtggtgaa cggggccggc
     1321 caggagccga ccacggcggg gacgcggttg gtcgcctccc agtcgcggta gtcccaccag
     1381 atccgctcgc cgccctgcag gtcgacgtcg gcggcgccga tcggcgactc gaccccgttg
     1441 acgaagaaga accagtcgta ggggtggccg tcgcgacggc cttcggtgta gccctcgatc
     1501 gacttgacga agccgccgcc gtagcgggtc tcgatctcgg cctcgccttc gagcacccgc
     1561 atcaccgtgt ccgactcggt cacctcgacc gaactccgga ccatcggttg cgagccgaat
     1621 tcccgggtca cggtcagctc gaccgagccg acctcctcgc cggggccaag gccgcagccg
     1681 gcggccgcga gcgctgccag gagcagcgcc gccgcgaccg ccgtgccacg gctgcggatc
     1741 actagctacc ggcggggcat ttgccgccga cgcagaccgc ggcgcgatcg gggatcaggt
     1801 cccctttcgt cgccagcagc cgcttcgggc cgctcagcgt caccgtcgcc ttgccggtgg
     1861 cgctggtcag gccgctggcg ccggtcacct tgaccccggc ggccggcttg cgcttgcctt
     1921 tttcgtcgta gctgtaggcg gtcaccgtga acgccttgcc cgcctgggct ttcgccggca
     1981 cgaccagggc cagctcttcc gggtaggcga acgtcttcgg gtccgattcg gcgagcgcga
     2041 agaggacctc gtcgccggcc ttgatcttgg tcgtgtcggc actgaccatc gttcccttgt
     2101 ggttcacctt caggtaccag gagagaccgc ccttggcgac gccgctgccg acaccgcaga
     2161 gcgccaggcc gaagtcgaag ctgtcgctga tcagcagcgg gttcagcgcc ggggtcgact
     2221 tggcgccgcg ggcgagcagc gccagcgcgt tgttttcctt cagcgcgacc gagtcgccgc
     2281 tgccgccgct gccggggccg aagcaggtcg ccttggggct ggtcttgacc gagatggcgc
     2341 cggtcgtgac cgtcttttcg gcgaggatct tgccgccctt gccgaccaca cgcaggtcgg
     2401 ccgaaaagcc tttggcggcg gcaacggaga cagacatcag ggacagggcc actaccagcg
     2461 cgcacgcggc agccagccgt agggaaatgg ttcttggcat taagccaccc cttcctcgag
     2521 ggttgaattg gctggccggg gacaggtctc ctggcttccg gggttggcga gtcgcacctt
     2581 cccggggttt tgtgtcgtc
//
LOCUS       DASRIG010000247         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2454853, whole genome shotgun sequence.
ACCESSION   DASRIG010000247 DASRIG010000000
VERSION     DASRIG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454853"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFB52_06760"
     CDS             <1..871
                     /locus_tag="VFB52_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZO06070.1"
                     /translation="FEMFVNRGIYHKGWTAVTRHSTPWAFAAELPAFDDDVWELYAPD
                     DWTQSNDLAAEQPEKLAELQRLFLIEATRYGVLPLDDRRVERFVPEIAGRPSLIKGNS
                     QILFGGMKRISENSMITIKNRSHSVTAQLVVPEGGAEGVVIAQGGAFGGWSIYLDEGR
                     PTYCYNLLGIARFKVAGAEPLGAGEHQVRIEFVYDGGGLGKGGDAALYLDGTKVGEGR
                     VEGTMPLIFSGDETTDLGSDLGTPVSDDYAAADSAFTGKVEWVQIDVDEAAEDPDHLI
                     SPEERFRVAMARQ"
     gene            complement(917..1708)
                     /locus_tag="VFB52_06765"
     CDS             complement(917..1708)
                     /locus_tag="VFB52_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO06071.1"
                     /translation="MASQLAALVGTHLRDRRRSLLAWGVPLGLMSAFIVAIFPSVEDA
                     LSQAISDYPPALKEAFGIGQLSNVEQYLYAEMLSLIVPLALGYLAVRAVASGLAGAAE
                     SGRLDVLLSAPVSRHRLCAAGFLASAVELAAVLAITGLLTGVGSLLAGAGLSASSALA
                     GFANVWPLALFFAGLGIVLCAFSLRTTVVTGAVAGVLVAMYVIDLVGRLDPGLDWVRY
                     ASVFKYYGNAVEDGIDPLAFIGVTLAAVALAALGAALFDRRDVGA"
     gene            complement(1708..>2429)
                     /locus_tag="VFB52_06770"
     CDS             complement(1708..>2429)
                     /locus_tag="VFB52_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06072.1"
                     /translation="RGSARLFGLDSRRESVAIRARLGNLPGDFGYGREATGREALTLL
                     ARLRGLDGLGRAEALAGRFHADLDRPLGQLSRGNRQKVGLIQATFHEPELLILDEPTG
                     GLDPLMQEEFLDLVGEERERGCAVFLSSHDLGEVERICDRVGIVRSGRLVAVERIADL
                     LGKARRQVTVELAEPAELPELRALPGVSEVEAAGSRITFKVAGDLDPVVKALANHHVV
                     DLEAVHPSLEEVFLGYYGEQE"
BASE COUNT          385 a          858 c          883 g          303 t
ORIGIN      
        1 cttcgagatg ttcgtcaacc gcggcatcta ccacaagggc tggaccgcgg tgacccgcca
       61 cagcaccccg tgggcattcg ccgccgagct gcccgcgttc gacgacgacg tctgggagct
      121 ctacgccccc gacgactgga cccagtccaa cgacctcgcc gccgagcagc ccgagaagct
      181 ggccgagctc cagcgcctgt tcctgatcga ggcgaccagg tacggggtgc tgccgctcga
      241 cgaccgccgg gtcgagcgct tcgtgcccga gatcgccggg cggccgagcc tgatcaaggg
      301 caactcgcag atcctcttcg gcggcatgaa gcggatctcg gagaactcga tgatcacgat
      361 caagaaccgc tcgcactcgg tcacggcgca gctggttgtg cccgagggcg gggcggaggg
      421 cgtggtcatc gcccagggcg gtgccttcgg cggctggagc atctacctcg acgagggccg
      481 gccgacctac tgctacaacc tgctcgggat cgcgcgcttc aaggtcgcgg gcgccgagcc
      541 actgggcgcg ggcgagcacc aggtgcggat cgagttcgtc tacgacggcg gcgggctcgg
      601 caaaggcggc gacgccgcgc tctacctcga cggcaccaag gtcggcgagg gccgggtcga
      661 gggcacgatg ccgctgatct tctccggcga tgagacgacc gacctcggct ccgacctcgg
      721 cacgccggtc agcgacgact acgccgcggc cgacagtgcc ttcaccggca aggtcgagtg
      781 ggtgcagatc gacgtcgacg aggccgccga ggaccccgac cacctgatct ccccggagga
      841 gcgcttccgg gtcgcgatgg ctcgccagta gggcagggcc gctcgggatc tgccggctgc
      901 gagcgggttt ctttcttcag gcgccgacgt cgcggcgatc gaacagcgcc gcaccaagtg
      961 cggcgagagc cacggccgcc agcgtcacgc cgatgaaggc gagcgggtcg atcccgtctt
     1021 cgaccgcgtt gccgtagtac ttgaagacgg aggcgtagcg gacccagtcg aggcccgggt
     1081 cgagccggcc gaccaggtcg atcacgtaca tcgccacgag cacaccggcg accgcgccgg
     1141 tgaccacggt cgtgcgcagc gagaaggcgc agagcacgat cccgaggccg gcgaagaaca
     1201 gcgccagcgg ccagacgttg gcgaacccgg cgagcgccga ggaagccgag agccccgccc
     1261 cggcgagcag gctgcccacg ccggtcagca ggccggtgat cgccagcacc gcggccagct
     1321 cgacggcgct ggcgaggaag ccggcggcgc agaggcggtg acgcgagacg ggggccgaga
     1381 gcagcacgtc gaggcggccg ctttcggcgg caccggcgag gccgctggcg accgcgcgca
     1441 ccgccaggta gccgagcgcc agcggcacga tcaggctcag catctcggcg tagaggtact
     1501 gctcgacgtt ggagagctgg ccgatcccga acgcttcctt cagcgccggc gggtagtcgc
     1561 tgatcgcctg gctcagcgcg tcttcgaccg aggggaagat cgcgacgatg aaggccgaca
     1621 tcaggcccag cggcactccc caggcgagca gcgagcgacg ccggtcgcgc aggtgggtgc
     1681 cgaccagcgc ggcgagttga gacgccatca ctcctgctcc ccgtagtagc cgaggaagac
     1741 ctcctcgagg ctcgggtgga ccgcctcgag gtcgacgacg tggtggttgg cgagcgcctt
     1801 cacaaccggg tcgagatcgc cggcgacctt gaacgtgatc cggctgcccg ccgcttcgac
     1861 ctcgctcacc cccggcagcg cgcgcagctc gggcagctcg gcgggctccg ccagctcgac
     1921 cgtgacctgg cgccgtgcct tgccgagcag gtcggcgatc cgctcgaccg cgaccagccg
     1981 gcccgagcgc acgattccca cccggtcgca gatccgctcg acctcgccga ggtcgtgcga
     2041 ggagaggaag acggcgcagc cgcgctcgcg ctcctcgccg accaggtcga ggaactcctc
     2101 ctgcatcaac gggtcgaggc cgccggtcgg ctcgtcgagg atcagcagct ccggttcgtg
     2161 gaaggtcgcc tggatcagcc cgaccttctg gcggttgccg cgcgagagct ggcccagcgg
     2221 ccggtcgagg tcggcgtgga agcggccagc cagcgcctcg gcgcgcccga gaccgtcgag
     2281 gccgcgcagg cgggccagca gggtcagcgc ctcgcgcccg gtcgcctcgc ggccgtagcc
     2341 gaagtccccc ggcaggttgc cgaggcgggc gcggatcgcg acgctctcgc ggcggctgtc
     2401 gagcccgaag aggcgggcgc tgccgcggg
//
LOCUS       DASRIG010000248         5680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_122449, whole genome shotgun sequence.
ACCESSION   DASRIG010000248 DASRIG010000000
VERSION     DASRIG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122449"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFB52_06775"
     CDS             complement(<1..346)
                     /locus_tag="VFB52_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06073.1"
                     /translation="MPDEGNLRLRFRQDEVVAELRAAFLSVPYPSGLRRLLAGRPGIE
                     AVLVEHIPSGLDAAAREAGVSYLDPRGNGRVVAPGLVYVVPPRGPLMHRMIGGDVPDD
                     PEAAPPKASRRVS"
     gene            575..2065
                     /locus_tag="VFB52_06780"
     CDS             575..2065
                     /locus_tag="VFB52_06780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO06074.1"
                     /translation="MADPGSHTTTLLIGGESVAGAGAPIAVENPYTTSTIVEVASAST
                     EQVDAAVATAREAWPGWARMPAGERCELLHEVARKLREGAEELARTMTAEGGKPLIEN
                     RDELEWTAAAFDYYAEIGRDHAGRVIPPIESTQLAMVVKDPLGVVGCIVPWNYPLLLL
                     AWKVAPALAAGNCVVAKPSELTPLSTLMLAPVFDHLPPGVVGLLAGAGDVGAQLAAHP
                     GIDCVAFTGSVATGKKVAEACIPRMARMNLEMGGKDAFIVCADVAPQLEVAARGGAWA
                     AFLNAGQVCTSAERFYVMEDVFDDYVDALVEHTRSLVLGDPFAVETDLGPMVSDPQRQ
                     KVIAQLEAATAAGAEVLIGGDDGGQAQGHFLTPAVVTGAAPDSALLTEETFGPIAPIV
                     PVSSLDEAIALANGIEFGLGANVYTADLENAVRCMKELRAGTVWINDPLTDNDAGPFG
                     GFKQSGLGRELGREGLEAFQETKHVHMESRIERKEWWYPYKEYGAQ"
     gene            2119..2802
                     /locus_tag="VFB52_06785"
     CDS             2119..2802
                     /locus_tag="VFB52_06785"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZO06075.1"
                     /translation="MRVARFERDRFAVRVVAFEPCSTLLDWCQANDAAHAIVGGFYMR
                     PGGPPLGELWIDGEARASEPIDPPWGELRACLHVDGGPVRLAPRHQLPPEPEGDLLQA
                     GPMLLAAGQTLIQRGQDEEGFSAGSRQFDSDITAGRYPRAALGLNDRELIAIVCEGRA
                     DEEAGLTMAELAAAMAGLGAEDAINLDGGGSASLVVDGDLVNTPREEHGLELVGGREV
                     ATALRFVPR"
     gene            2852..4237
                     /locus_tag="VFB52_06790"
     CDS             2852..4237
                     /locus_tag="VFB52_06790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO06076.1"
                     /translation="MPSTPNKLLMGIYFYPRGGSAHVCRALAREFERNETEVTVLSGS
                     RSDVGEHALAASFYAGLDLHTADFTPALKTSEPVRFPHATGAVPMHGSYEDRPGAEDP
                     VLASLDDEAYENQVEAWIAELDSAGAGEADLLYLHHLTPLNEAAARAFPELPVLGHVH
                     GTELLMLEQIAAGAPPGWSHAEAWAARIRGWADECERIVVANPAGLERAALALGLDPD
                     RFTCAPNGYDPGFAPRKVDRCAHWRRHLVERPQGWRPGAPAGSVAYRDEEIEALEGTV
                     LIYVGRFTEIKRLPLLIEAFAAARARCAGPVSLVLLGGHPEEWEGEHPIETIERLDCP
                     DVFLAGWHSHADLPGFLQAADLLVHASAHEQFGQVLVEAMACELPVVAVNRGGPAAIV
                     DDPETGWLVPPDDVDALTQAMVAAVNDKEDRRLRGQRARGEVVGIYAWEQIGRTLAAV
                     AGDIPSAATQA"
     gene            4299..>5680
                     /locus_tag="VFB52_06795"
     CDS             4299..>5680
                     /locus_tag="VFB52_06795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO06077.1"
                     /translation="MTRLTPRLQRWIFVCGALLLAAFAAFTLHAATDLGGGALDDLFG
                     IWVYDALMLGAAASCLARAALIRRERLAWALLGAGLAAWTGGEIYFSAVFSGSESVPI
                     PSLADAGYLAFYPLAYVALIALMRERIGSFPATRWLDGLIVGSAVAALAAALALQPIV
                     NESTNGDTAAVITNLAYPIADLTLLTLVVTAAAFTGWRPGLSWLTLGAGLVVLAASDG
                     LYLLQSAQGTYVEGGILDAAWPLGALLLAAAAWLSPGPRRRTVQPRGLRVAIVPTAAA
                     LVAIAVQSAERYALIPSIAAALSLFTLIVVVLRLALSFRDTQASLETSEREALTDPLT
                     GLGNRRRLMDDLARSTEEPSPNGQLRLLVLFDLDGFKAYNDSFGHPAGDALLARLGGR
                     LNEFATPYGEAYRLGGDEFCLLAECTAGAVDSLLAGGSAALSERGDGFRVTTSQGSVI
                     LPSEARTREEA"
BASE COUNT          793 a         2157 c         1925 g          805 t
ORIGIN      
        1 gcgagacccg gcggctcgcc ttcggcggcg ctgcctcggg gtcgtcggga acgtcgccac
       61 cgatcatccg gtgcatgagc ggtccccgcg ggggaacgac gtagacgagg ccgggtgcga
      121 cgaccctgcc gttgccgcgc gggtccaggt aagagacccc ggcctcgcgg gcggccgcgt
      181 ccaggcccga cggaatgtgc tcgacgagga ctgcctcaat ccctggtcta cctgccagga
      241 gccgccgcag acccgatggg tacggcaccg agaggaatgc ggcccgaagc tcagcgacga
      301 cctcatcctg cctgaagcgc aggcgcaagt tgccctcatc gggcatgacc tcgagcccta
      361 ccccaccctc cagctctgga ggctgaaata ggaaacgatc tctccagtca ccactgtttg
      421 caggcatttt gtcctcaatt ggctacttgt cttgcaagac aaacatatca tcgtcgacaa
      481 agtacttgtc tgtaaataca gaattgtctt gcaagacaac ggccacccag cgcacaaacg
      541 aacgccccgc cggatagata gcctgagggc ctcgatggcg gaccccggct cgcacacgac
      601 gacgctcctg atcggcggtg agtccgtggc cggggccggc gcgccgatcg cggtcgagaa
      661 cccgtacacg acctcgacga tcgtcgaggt cgcgagcgct tcgaccgagc aggtcgacgc
      721 cgccgtcgcc accgcccgcg aggcctggcc cggctgggcg cggatgcccg ccggcgagcg
      781 ctgcgagctg ctgcacgagg tcgcgcgcaa gctgcgcgag ggcgccgagg agctggcgcg
      841 gacgatgacc gccgagggcg gcaagccgct gatcgagaac cgcgacgagc tcgagtggac
      901 cgccgccgcg ttcgactact acgcagagat cggccgcgac cacgccggcc gcgtgatccc
      961 gccgatcgag tcgacccagc tggcgatggt ggtcaaggac ccgctcggcg tggtcggctg
     1021 catcgtgccc tggaactacc cgctgctgct gctcgcctgg aaggtggcgc cggcgctcgc
     1081 cgccggcaac tgcgtcgtcg ccaagccctc ggagctgacc ccgctctcga ccctgatgct
     1141 ggcccccgtc ttcgaccacc tgccgcccgg cgtggtcggc ctgctcgccg gcgccggcga
     1201 cgtcggcgcc cagctcgccg cccaccccgg catcgactgt gtcgccttca ccggctcggt
     1261 cgcgaccggc aagaaggtcg ccgaagcctg catcccgcgg atggcgcgga tgaacctgga
     1321 gatgggcggc aaggacgcct tcatcgtctg cgccgacgtc gccccgcagc tcgaggtcgc
     1381 cgcccgcggc ggcgcctggg ccgccttcct caacgccggc caggtctgca cctcggccga
     1441 gcgcttctac gtgatggagg acgtcttcga cgactacgtg gacgccttgg tcgagcacac
     1501 ccgcagcctc gtgctcggcg accccttcgc ggtcgagacc gacctcggcc cgatggtctc
     1561 cgacccccag cgccagaagg tcatcgccca actggaagca gcgaccgcgg ccggcgccga
     1621 ggtcttgatc ggaggcgatg acggcggcca ggcgcagggc cacttcctca cccccgccgt
     1681 ggtcaccggc gccgcccccg acagcgccct gctgaccgag gagaccttcg gtccgatcgc
     1741 cccgatcgtc ccggtctcct cgctcgacga ggcgatcgcg ctcgccaacg gaatcgagtt
     1801 cggcctcggc gccaacgtct acaccgccga cctcgagaac gcggtccgct gcatgaagga
     1861 gctgcgcgcc ggcaccgtct ggatcaacga cccgctgacc gacaacgacg ccggcccgtt
     1921 cggcggcttc aagcagtccg ggctgggacg cgagctcggc cgcgagggcc tcgaggcctt
     1981 ccaggagacc aagcacgtgc acatggagtc gcggatcgag cgcaaggaat ggtggtaccc
     2041 gtacaaggag tacggggcgc aatagctccc aggctcgaga gcgtcaggct cgacctggcc
     2101 gacggcgccc ggaccaccgt gcgcgtcgcc cgcttcgagc gcgaccgctt cgcggtccgc
     2161 gtggtcgcct tcgagccctg ctcgaccctg ctggattggt gccaagcgaa cgacgccgcg
     2221 cacgcgatcg tcggcggctt ctacatgcgg ccgggagggc cgccgctcgg cgagctctgg
     2281 atcgacggcg aggcccgagc gagcgagccg atcgacccac cctgggggga gctccgagcc
     2341 tgcctgcacg tcgacggcgg gccggttcgg ctggcgcccc gccaccagct cccgcccgag
     2401 cccgagggag acctcctgca ggcaggaccg atgctcctcg ccgccggcca gacgttgatc
     2461 cagcgcggcc aggacgagga gggattctcg gcgggctcgc ggcagttcga ctccgacatc
     2521 accgcgggcc gctacccccg cgccgccctc ggcctcaacg accgggagct gatcgcgatc
     2581 gtctgcgagg gccgcgccga cgaagaggcg gggctgacga tggcggagct ggcggcggcg
     2641 atggccgggc tcggcgccga ggacgcgatc aacctcgacg gtggcggctc cgcctcgctg
     2701 gtcgtcgacg gcgacctcgt caacacgccg cgggaggagc acggcctcga gctggtcggc
     2761 ggccgcgagg tcgccaccgc cctgcgtttc gtccctcgct aggccccctc ccgccgggcg
     2821 cgattaggtg ccgggagtag cctcggtgta gatgccctct actccgaaca agctgctgat
     2881 gggcatctac ttctatcccc gcggcggctc agcccacgtc tgcagggccc tcgcacgcga
     2941 gttcgagcgc aacgagaccg aggtgacggt gctctccgga tcgcggtcgg acgtcggcga
     3001 gcacgcgctg gccgccagct tctacgccgg gctcgacctg cacaccgcgg acttcacccc
     3061 ggccctgaag acctccgagc cggtccgctt cccgcacgcg accggcgcgg tgccgatgca
     3121 cgggtcctac gaggaccggc ccggggccga ggacccggtg ctcgccagcc tcgacgacga
     3181 ggcctacgag aaccaggtcg aggcctggat cgccgagctc gacagtgccg gcgccggcga
     3241 agccgacctg ctctacctcc atcacctgac cccgctgaac gaggcggccg ctcgcgcctt
     3301 ccccgagctg ccggtgctcg gccacgtgca cggcaccgag ctgctgatgc tcgagcagat
     3361 cgccgccgga gcgccgccgg gctggagcca cgccgaggcc tgggcggcgc ggattcgcgg
     3421 ttgggcggac gagtgcgagc gcatcgtcgt cgccaacccg gccgggttgg agcgcgccgc
     3481 gctggctctg ggactcgacc ccgaccgctt cacctgcgcg ccgaacggct acgacccagg
     3541 cttcgccccg cgcaaggtcg atcgctgcgc ccactggcgc cggcacctgg tcgagcgccc
     3601 gcagggctgg cggccgggcg cgccggccgg cagcgtcgcc taccgcgacg aggagatcga
     3661 ggcgctggag gggaccgtcc tcatctacgt cggccgcttt accgagatca agcgcctgcc
     3721 actgctgatc gaggccttcg ccgcggcccg cgcacgctgc gccggccctg tctccctggt
     3781 cctgctcggc ggccatccgg aagagtggga gggcgagcat ccgatcgaga cgatcgagcg
     3841 gctggactgc ccggatgtct tcctcgccgg ctggcattcc cacgcggacc tgccgggctt
     3901 cctgcaggcc gccgacctgc tcgtccatgc ctcggcgcac gagcagttcg ggcaggttct
     3961 ggtcgaggcg atggcctgcg agctgccggt ggtcgccgtc aaccgcgggg gcccggcggc
     4021 gatcgtcgac gaccccgaaa ccggctggct ggtcccgccc gacgacgtcg atgcgctgac
     4081 gcaggcgatg gtcgcggcgg tcaacgacaa ggaggaccgc cgcctgcggg gacagcgtgc
     4141 ccgcggcgag gtggtcggca tctacgcctg ggagcagatc gggcgcacgc ttgcagcggt
     4201 cgccggcgac atccccagcg ccgccacaca ggcctgaatt cgccgaaatt cggccgcatg
     4261 ggatcaagtt cccgatccgg tcgctcgata cgagacgggt gaccaggctc acccctcggc
     4321 tacagcgctg gatcttcgtc tgcggggcgc tgctgctcgc cgccttcgct gccttcacgc
     4381 tgcacgcggc gaccgacctc ggcggcggcg cgctcgacga cctcttcggg atctgggtct
     4441 acgacgccct gatgctcggt gccgccgcct cctgcctggc gcgggcggcc ctgatccggc
     4501 gcgaacggct cgcctgggcg ctgctcggcg ccggcctggc cgcctggacc ggcggcgaaa
     4561 tctacttctc ggctgtgttc tcaggatccg aatcggtgcc gatcccctcg ctcgccgacg
     4621 ccggctacct cgccttctac ccgctcgcct acgtcgccct gatcgcgctg atgcgggagc
     4681 ggatcggctc cttcccggcg acccgctggc tcgacggcct gatcgtcggc tccgccgtcg
     4741 ccgcgctcgc cgcggcgctc gccctgcagc cgatcgtcaa cgaaagcacc aacggcgaca
     4801 ccgccgccgt gatcaccaac ctcgcctacc cgatcgccga cctcaccctg ctgaccctgg
     4861 tcgtcaccgc cgcggcgttc accggctggc gccccgggct gagctggctc accctcggcg
     4921 ccggcctcgt cgtgctcgcc gcctccgacg gcctctatct gttgcaatcg gcgcagggga
     4981 cctatgtcga aggcggcatc ctcgatgccg cctggccgct cggcgcgctg ctgctcgcgg
     5041 ccgccgcctg gctgtcgccc ggcccgcgcc ggcgcaccgt gcagccgcgc ggcctgcggg
     5101 tcgcgatcgt gcccacggcg gcggcgctgg tcgcgatcgc cgttcagtcc gccgagcgct
     5161 atgccttgat cccgagcatc gccgccgcgc tctcgctgtt cacgctgatc gtcgtcgtcc
     5221 tgcgcctggc gctctccttc cgcgacaccc aggcgagcct ggagacgagc gaacgcgagg
     5281 ccctgaccga cccgctgacc gggctcggca accgccgccg cctgatggac gacctcgcgc
     5341 gctcgaccga ggagccgtcg ccgaacggcc agctgcgcct gctcgtgctc ttcgacctcg
     5401 acggcttcaa ggcctacaac gacagcttcg gccaccccgc cggcgacgcc ctgctggccc
     5461 ggctcggcgg ccggctgaac gagttcgcca cgccctacgg cgaggcctac cggctcggcg
     5521 gcgatgagtt ctgcctgctc gccgagtgca ccgccggcgc cgtcgacagc ctgctcgccg
     5581 gcggctcggc ggcgctctcc gagcgcggcg acggcttccg cgtcaccacc tcccagggca
     5641 gcgtgatcct gcccagcgag gcgcgcaccc gcgaggaggc
//
LOCUS       DASRIG010000249         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_900051, whole genome shotgun sequence.
ACCESSION   DASRIG010000249 DASRIG010000000
VERSION     DASRIG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900051"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFB52_06800"
     CDS             complement(<1..370)
                     /locus_tag="VFB52_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO06078.1"
                     /translation="MSVGAAPPPVEPQPLRDGDEMAPVVTEPFTRTDFVRYAGSGGDY
                     HPIHHDEEFARAAGMPTVFGMGMLHAGMLGLALARWVGPENIRSFGTRFTGQVWPGDV
                     LTFRARVTGVEDGLASIELSA"
     gene            complement(367..807)
                     /locus_tag="VFB52_06805"
     CDS             complement(367..807)
                     /locus_tag="VFB52_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZO06079.1"
                     /translation="MSGALDSVRFPVERSKLMELARTFKDPDPLWHDPEAAAEAGFDS
                     VPVPPTATVLADHWRERGATFHAQAAGLDLPRVLHGEVSWEFLRPVQLGDELTAVSSL
                     AGDETRQGKRGGEMRLVTVETSYTDAAGELVARRRDTLIETGPR"
     gene            888..2030
                     /locus_tag="VFB52_06810"
     CDS             888..2030
                     /locus_tag="VFB52_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06080.1"
                     /translation="MPAERMLPTPEAEEIVRLARDLAQDTLAPRVAAAEAEEAFPRDV
                     FATLGQLGFLGMPYPEELGGAGQPYEVYLQALEEIAAVWASVGVGISVHIMSCYPLAT
                     WGTEEQKGRWLEEMLAGELLGGYCLSEADAGSDPAAMRARAVREGEGYRVSGAKAWVT
                     HGGEADFYTLFARTSEDRSRGVSCFLVPGDAPGLSAQSPEQKMGLTGSTTATLDYDGV
                     EVEAERRVGEEGEGLAIALAALDSGRLGIAAVATGLAQGALDHAVAYAKERETFGKPI
                     IDHQGLGFLLADMAAAVSSARAVTLEAARRRDRGLAFSRQASIAKLVATDAAMKVSTD
                     AVQVLGGFGYTKEFPVERMMREAKVMQIFEGTNQIQRLVIARHLSR"
     gene            complement(2027..2644)
                     /locus_tag="VFB52_06815"
     CDS             complement(2027..2644)
                     /locus_tag="VFB52_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06081.1"
                     /translation="MLEPKQARSHATRRRLLDAAVQVLVEEGYAGISASTVARRAGVS
                     RGAHQHHFANRQTLVVESVRHMSARELEALQERIAALPHGESRVAGALDFIFEMYSGT
                     FFAAILELSLAARREPELRTAIADEERSMSLTVQQLGTEIFGKDAFLDPDLGHRWTTA
                     IATIRGLAVLRLLRHSKETIERQWRYARGEILATLMRAKAEAPPS"
BASE COUNT          413 a          997 c         1047 g          421 t
ORIGIN      
        1 cggcgctcag ctcgatgctc gcgaggccgt cttcgacgcc ggtcacgcgg gcgcggaagg
       61 tgaggacgtc gccgggccag acctggccgg tgaagcgggt cccgaaggag cggatgttct
      121 ccgggccgac ccagcgggcg agcgccagtc cgagcatccc ggcgtgcagc atgcccatcc
      181 cgaagacggt cggcatcccc gccgcgcggg cgaactcctc gtcgtggtgg atcgggtggt
      241 agtcgccgcc ggagcctgcg tagcggacga agtcggtgcg ggtgaagggc tcggtgacga
      301 ccggcgccat ctcgtcgccg tcgcgcagcg gctgcggctc gaccggcggc ggggcggccc
      361 cgacgctcac cgcggccccg tctcgatcag ggtgtcgcgg cggcgcgcga cgagctcgcc
      421 ggcggcgtcg gtgtaggagg tctcgaccgt gaccaggcgc atctcgccgc cgcgcttgcc
      481 ctggcgggtc tcgtcgccgg ccaggctgga gacggcggtc agctcgtcgc cgagctggac
      541 gggccgcagg aactcccagc tgacctcgcc gtgcaggacg cggggcaggt cgaggccggc
      601 cgcttgcgcg tggaaggtgg cgccgcgctc gcgccagtgg tcggcgagca cggtcgcggt
      661 cggcggcacc gggaccgagt cgaagccggc ctcggcggct gcctcggggt cgtgccagag
      721 cgggtccggg tctttgaagg tccgcgccag ctccatcagc ttcgagcgct cgaccgggaa
      781 gcgcacgctg tccagcgcgc cgctcatggc ctcggactcc gctcccatgg ccgcatcata
      841 caaaaaaagc attcctgatg gatctttttg gttagtatcg cgccccgatg cccgccgaac
      901 gcatgctccc cacccccgag gccgaggaga tcgtccgcct cgcccgcgac ctcgcccagg
      961 acaccctcgc ccccagggtc gccgcagccg aggccgagga ggccttcccc cgcgacgtct
     1021 tcgccacgct cggccagctc ggcttcctcg gcatgcccta ccccgaggag ctcggcggcg
     1081 ccggtcagcc ctacgaggtc tacctgcagg cgctcgagga gatcgccgcg gtctgggcct
     1141 cggtcggggt cggcatcagc gtccacatca tgtcctgcta cccgctcgcg acctggggca
     1201 cggaggagca gaaaggccgc tggctggagg agatgctggc cggcgagctg ctcggcggct
     1261 actgcctctc ggaggccgac gccggctcag accccgccgc gatgcgcgcc cgcgccgtgc
     1321 gcgagggcga gggctaccgc gtctccggcg ccaaggcgtg ggtcacccac ggtggcgagg
     1381 ccgacttcta cacgctcttt gcccgcacct cggaggaccg ctcgcgcggg gtctcctgct
     1441 tcctcgtccc cggcgacgct cccgggctga gcgcccagag ccccgagcag aagatgggcc
     1501 tgaccggctc gaccacggcg acgctcgact acgacggggt cgaggtcgag gcagagcggc
     1561 gggtcggcga ggagggcgag ggcctggcga tcgcgctggc ggcgctcgac agcggccggc
     1621 tcgggatcgc cgccgtcgcc accggcctcg cccagggcgc gcttgaccac gcggtcgcct
     1681 acgcgaagga acgcgaaacc ttcggcaagc cgatcatcga ccaccagggg ctcggcttcc
     1741 tgctcgccga catggccgcc gcggtctcct cggcccgcgc ggtgacgctg gaagcggcgc
     1801 gccggcgcga ccgcgggctg gcgttctcgc gccaggcctc gatcgccaag ctggtggcca
     1861 ccgacgcggc gatgaaagtg agcaccgacg cggtccaggt gctcggcggc ttcggctaca
     1921 ccaaggagtt cccggtcgag cggatgatgc gcgaggccaa ggtgatgcag atcttcgagg
     1981 gaaccaacca gatccagcgc ctggtgatcg ccaggcacct gagccgctag ctcggcggcg
     2041 cctcggcctt ggcccgcatc agggtggcga ggatttcgcc gcgggcgtag cgccactggc
     2101 gctcgatcgt ctctttggag tggcgcagca gacgcagcac tgcgagcccg cgaatcgtcg
     2161 cgatcgccgt cgtccagcgg tgcccgaggt cggggtcgag gaaggcgtcc ttgccgaaga
     2221 tttccgtccc gagttgctgc acggtcagcg acatcgagcg ctcctcgtcg gcgatcgcgg
     2281 tgcgcagctc gggctcgcgg cgggcggcga gggagagctc gagaatcgcg gcgaagaagg
     2341 tgccgctgta catctcgaag atgaagtcga gcgcgccggc gacccgcgac tcgccgtggg
     2401 gcagggcggc gatccgctcc tgcagggcct cgagctcacg cgccgacatg tggcggaccg
     2461 actcgacgac cagggtctgt cggttggcga agtggtgctg gtgggcgccg cggctgaccc
     2521 cggcgcggcg ggccacggtc gaggccgaga tcccggcgta gccttcttcg accaggacct
     2581 ggacggcggc gtcgagcaac cgccgccggg tggcgtggct gcgcgcctgt tttggttcga
     2641 gcatgttcat gggctgggaa aaaaggcagc cgcgaggaga tttcggcgca tcaattcagc
     2701 ggctccgccg catatcgtag tcccaccttc cccggacgtg attcccaagc ggcatcgtag
     2761 caaaaatcca gcgacgatgc ttgttttttt ggtggaattc gactcagcga tacccccttg
     2821 acagagccga atggcatcgc ttaggcttct gtaccgatcg ctcagtacag ggagcgga
//
LOCUS       DASRIG010000250         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2196003, whole genome shotgun sequence.
ACCESSION   DASRIG010000250 DASRIG010000000
VERSION     DASRIG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196003"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..869)
                     /locus_tag="VFB52_06820"
     CDS             complement(246..869)
                     /locus_tag="VFB52_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06082.1"
                     /translation="MIRKAIYRIHPASVLAMLALFVALGGGAYAAVGKIGSADIKAGA
                     ITKPKIKKDAVTGAKVKESTLGIVPNAAKLAGKEPSAFESKGFGGSGDAAFVNLTSDS
                     VTEVASQALPAGTYLVLARGGLNNNSGEDIPLGQGCTLSAGGTSQSIDFTGLAANGAP
                     GDREEFTLFVVATLPSPGSAVLSCETTSKWKSGNVIEPNIAAVSLQP"
     gene            1033..2556
                     /locus_tag="VFB52_06825"
     CDS             1033..2556
                     /locus_tag="VFB52_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO06083.1"
                     /translation="MADVVEGSQSGLEAEEARLARAAAAGDGEAFATLYERYSQRAYN
                     LSLRLCGSDEDAADAVQEAFVSVIRRLKEMEAERELAFGSYLFTATRNATYDLLRRAQ
                     RTRPSDAIAESATPLGAGAGGLGLDPGAPEEDPDRNVLLASQQEEVRAANARLPERQR
                     EALALRELEEMSYDEIAAVMEMNRNSVAQLISRARISLRDELRGTALASVTAASPECE
                     RALPLIAARDDGQLDPDSEDASWLAAHLASCETCLVATAAMEEAGVSYRAWIPVAVAP
                     WIFKETMARAAELSGSDWSGIAECRLADPTDPSRIPGLPRAYRTAAAVERGPRRRRAA
                     LVGVLAVLLLLGGSVVALSAGGGKDPAPQPAGDVTEVPVTEPVDEPEPQPAPVEKEKE
                     DEKLEPRKGQAPPDSPEPLPLTEAPPTNTGPTPAESKTKPGPGPDANGNPNQGKGGVG
                     QPSPGRDPQPDPGTEPVPPPPPPPPAGEEPVPPPPPPPPVPGGPGDLKNPPGPPKPG"
     gene            2666..>3638
                     /locus_tag="VFB52_06830"
     CDS             2666..>3638
                     /locus_tag="VFB52_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZO06084.1"
                     /translation="MPQHHLTAPRGAELAPKSEFVEGRFGRLFRNLPSFVPGDEALTA
                     LAGAMVEPDGAGDLDSPIPAGYTYLGQFIDHDITFDPASSLQRLQDPDGLHDFRTPRL
                     DLDSLYGDGPNNDPYLYDTRIDKGATSFLIGSNGIGELDLPRNVLPPDANNVPQGRAL
                     IGDPRNDENVIVGQLHLLFLRFHNKVVELLREEIGNPEQLFAEAQRLVRWHYQWIVVN
                     DFVRRICGDGVVDDILRPDTYVTAGGVGEILRAHLQFYKWKREPFMPVEFSVAAYRFG
                     HSMIRGGYKLNGDIPAVPLFTEGPLQNEHQDLRGFRTLATQWTIDWDF"
BASE COUNT          553 a         1313 c         1244 g          528 t
ORIGIN      
        1 cgttcaccca ggtctacgcg ccgagcgaca ccgatgcggt cgcgatcgag ccgatgacgg
       61 cgccgaccga cgccctgcgc agcggcgccg acctgccctg ggtcgagccc ggcgagagct
      121 tcgccgccag gttctcgatc cgcgtgggct gagcggtttt gccccgcatg tggcgcgccc
      181 tgaacagcga cggggccggg caatcgcccg gcccctcgtg ctgatcggaa agatcgccgc
      241 gaaccctagg gctgcaggga gacggcagcg atgttcggct cgatcacgtt gccgcttttc
      301 catttcgagg tggtctcgca gctcagcacc gcgctgccgg ggctcggcag ggtggcgacg
      361 acgaagaggg tgaattcttc gcggtcgccg ggggcgccgt tggcggcgag gccggtgaaa
      421 tcgatcgact gcgaggtccc gcccgcgctc agcgtgcacc cctgccccag cgggatgtct
      481 tcgccggagt tgttgttgag accgccgcgg gcgagcacca ggtaggtgcc ggccggcagc
      541 gcctggctgg cgacctcggt gacgctgtcg gaggtgaggt tgacgaaggc ggcgtcgccg
      601 ctgccgccga agcccttcga ctcgaacgcg ctcggctcct tgccggccag cttggcggcg
      661 ttggggacga tgccgagcgt cgactccttc accttggcgc cggtgaccgc gtccttcttg
      721 atcttcggct tggtgatcgc gccggctttg atgtcggcgc tgccgatctt gccgaccgcc
      781 gcgtaggcgc cgccgccgag ggcgacgaac agggccagca tcgccagcac tgaggcgggg
      841 tggatgcggt agatggcttt gcggatcatg gttgacctcc tggggtcgtt gtggtgcgct
      901 ttcgtgcccc tccaacgggc caacccgggc cgaattacgc ggccgccgaa atctttttca
      961 gggcggcgta atttccgctt gccagagcgg ttggacgggt atgacgagcg aggactcagt
     1021 accttggagg cgatggccga cgtggtggag ggcagccaga gcgggctcga ggccgaagag
     1081 gcgcggctgg cgcgcgccgc ggcggctggc gacggcgagg ccttcgcgac gctctacgag
     1141 cgctactccc agcgcgccta caacctctcg ctgcgcctct gcggctccga cgaggacgcc
     1201 gccgacgcgg tccaggaggc gttcgtgagc gtgatccgcc gcctcaagga gatggaggcc
     1261 gagcgcgagc tcgccttcgg ctcctacctg ttcaccgcga cccgcaacgc gacctacgac
     1321 ctgctgcgcc gggcgcagcg gacgcggccg agcgacgcga tcgccgagtc ggcgacgccg
     1381 ctcggggcgg gcgccggcgg cctcggcctc gaccccggcg cccccgagga ggaccccgac
     1441 cgcaacgtgt tgctcgcctc ccagcaagag gaggtgcggg cggcgaacgc gcgcctgccc
     1501 gagcgccagc gtgaggcgct ggccctgcgt gagctcgagg agatgtccta cgacgagatc
     1561 gccgcggtga tggagatgaa ccgcaactcc gttgcccagc tgatctcacg ggcgcggatc
     1621 agcctgcgcg acgagctgcg cggcaccgcc ctcgcctcgg tcaccgccgc cagccccgag
     1681 tgcgagcggg cgctgcccct gatcgccgcc cgcgacgacg gccagctcga ccccgactcc
     1741 gaggacgcga gctggctggc cgcgcacctg gcgagctgcg agacctgcct ggtcgcgacc
     1801 gcggcgatgg aagaggcggg cgtctcctac cgcgcctgga tcccggtcgc ggtcgccccc
     1861 tggatcttca aggagacgat ggcgcgggcc gccgagctga gcggcagcga ctggagcggg
     1921 atcgccgagt gccggctcgc cgacccaacc gacccgtcgc gcatccccgg cctgccgcgc
     1981 gcctaccgca ccgccgccgc ggtcgagcgt ggcccgcgcc ggcgccgcgc ggcgctggtc
     2041 ggcgtgctcg cggttctact cctgctcggc ggctcggtcg tcgcgctctc ggccggcggg
     2101 ggcaaggacc cagcaccgca gccggccggt gacgtcaccg aggttccggt caccgagccg
     2161 gtcgacgagc ccgagccgca gccggccccg gtcgagaagg aaaaagagga cgagaagctg
     2221 gagccgagga aggggcaggc tccaccggat tcgcccgagc cactgccact gaccgaagcc
     2281 ccgcccacga acaccggccc gaccccggcc gagtcgaaga cgaagccggg ccccgggccg
     2341 gacgcgaacg ggaacccgaa tcagggcaaa ggcggcgtcg gacagccctc gccgggccgc
     2401 gacccccagc ccgaccccgg caccgaaccg gtcccgccgc cgcctccgcc accgcccgcc
     2461 ggcgaagaac cggtcccgcc gccgccccca ccgccaccgg tccccggcgg cccgggcgac
     2521 ttgaaaaacc cgccgggccc gccgaaaccc ggctaagacg tcgggtcccc cagaaaacta
     2581 agctgttctt gactggtctg gtggttccgg cgagtctcgc gtctccctga ccgcgtcgtg
     2641 ctcgccgcct cgcaaaggag acgcaatgcc ccagcaccac ctgaccgcgc cgcgtggcgc
     2701 ggagctcgcc cccaagtccg agttcgtcga ggggcgcttt ggccggctct tccgcaacct
     2761 tccctcgttc gtccccggcg acgaagcgct gaccgcgctc gcgggagcga tggtcgagcc
     2821 ggacggcgcg ggagacctcg acagcccgat tcccgcggga tacacctatc tcggccagtt
     2881 catcgaccat gacatcacct tcgacccggc gtcgagcttg cagcgactgc aggatcccga
     2941 cggtctgcac gacttccgga cgccgcgcct ggacctcgac tcgctctacg gcgacggccc
     3001 caacaacgat ccctacctct acgacacacg gatcgacaag ggtgcgacga gtttcctgat
     3061 cggcagcaac ggcatcggtg agttggacct gccccgcaac gtcctaccgc cggacgccaa
     3121 caacgtcccc cagggtcgtg cgctgatcgg cgacccgcgc aacgacgaga atgtgatcgt
     3181 cggccagctt cacctgctgt ttttgcgctt tcacaacaag gtcgtcgagc tactgcggga
     3241 agagatcggc aaccccgaac agctcttcgc ggaggcccag cgcctggttc gctggcacta
     3301 ccagtggatt gtcgtcaacg acttcgtgcg ccggatctgc ggtgacggtg tggtcgacga
     3361 catcctgcgg ccggacacct atgtcacggc tggcggcgtg ggcgagatcc tccgtgccca
     3421 tctgcagttc tacaagtgga agcgcgaacc gttcatgccg gtggagttct ccgtcgccgc
     3481 ttaccgcttt gggcactcga tgatccgcgg gggctacaaa ctgaatggcg acatccccgc
     3541 cgtgccgctg ttcaccgaag ggcctttgca gaacgaacac caggacctgc gcggcttccg
     3601 cacccttgcg acccaatgga cgatcgactg ggacttct
//
LOCUS       DASRIG010000251         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2455439, whole genome shotgun sequence.
ACCESSION   DASRIG010000251 DASRIG010000000
VERSION     DASRIG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455439"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFB52_06835"
     CDS             complement(<1..312)
                     /locus_tag="VFB52_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06085.1"
                     /translation="MPPALKLRHRFRSIVAGLESGHASTRGPEAAKGGKRRSLPGGWL
                     NWALGLLCLVVLAIAITSIGSSSSGATAEQRTAEVGRGVVQSTVSGNGSLEAAEQVEL
                     NF"
     gene            complement(388..1752)
                     /locus_tag="VFB52_06840"
     CDS             complement(388..1752)
                     /locus_tag="VFB52_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO06086.1"
                     /translation="MPIRRRLAVAAAVAVAIAIATASLAAYLAVRSQLRGEVDSALRE
                     RAEGVQLIVETEGPLQAPPDRQDLPEPPQARFGGAAGFVQFLEPDGSVKAQAPPGAER
                     LPVSPQARAVATGEESGDAYEDETVDGDHLRVLTAPLEGGGAVQVARPLDEVDSVLDR
                     LILILVIVTIGGIGLAAVLGTLVSRASLAPVRRFTDETESILEGSDLSRRLPDGGEDE
                     LGRLARTYNATLDALQSSADAQRQMVSDASHELRTPLASLKANVELLLRGEQRLGGAD
                     REELERELVEQIDELTLLVDDVVELARRGEPEPMLLDGVELSEIVAGEVERARRHAPG
                     LTFDVQLEPCAIRGVPERLARAVHNLLDNAAKWSPDGSTVEVTLAAGVLRVRDHGPGF
                     APEDLPFVFDRFYRARAARSQRGSGLGLAIVRQTAEAHGAAIEAANAPGGGAVLILRF
                     PLDE"
     gene            complement(1752..2447)
                     /locus_tag="VFB52_06845"
     CDS             complement(1752..2447)
                     /locus_tag="VFB52_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06087.1"
                     /translation="MLGSVMEVLIVDDEYPLRAALRRALALEGYGVTDAASGEEAIEL
                     LKERSWDAMVLDVLMPGLSGVDVCKRLRAAGERIPILMLTARETVADRVAGLEAGADD
                     YLVKPFALEELLARLKALLRRTEASGDDEQPLRYEGILLDPVRYEVKVGTRPVDLTRT
                     EFSLLHLLMRHQGRVLSRTTINEQVWGYDFGAASNSLGVYVGYLRRKLDEAGAPSLIQ
                     TVRGVGYVLRRPR"
     gene            complement(2936..3829)
                     /locus_tag="VFB52_06850"
     CDS             complement(2936..3829)
                     /locus_tag="VFB52_06850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO06088.1"
                     /translation="MRTDSRLEEQGPIKQLGSSDLLAALSLVQSGTVYDLEVERFRGM
                     PLHPAHPQMEIIAFRTPQGIQNQGDQEWLNSKNGENMAFISDLVVGTVHSGTHIDALA
                     HVTVGEDNHWFGGNKAAEKLGDFGPLVCDAAAIPTIITRGVLLDVAGHRGVPVLPKAE
                     GVSAEELQEVAAAQGTELRRGDTVLIRTGYVGLYPDAERMAEHAGPGINKGAAIWLVE
                     QGAVAVAGDTEALEQGPCADPENPHAVHTVLLVENGIYIMEMLNMEQLAADKVREFLF
                     VALPCKIRGATGSMIRPVALV"
     gene            3994..4764
                     /locus_tag="VFB52_06855"
     CDS             3994..4764
                     /locus_tag="VFB52_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO06089.1"
                     /translation="MSQPESVVSAREGKLLIITINRPQARNAVDLSVAEGLEAAVAEL
                     DADSDLAVGIVTGAGGTFCAGMDLKAFARGERPYTENGGFAGIVERPPRKILIAAIEG
                     YALAGGLEIALSCDLLVAARDAKLGIPEVGVGLIAAGGALLRLPSRIPYHLAMEMAVT
                     GEPITAEQAHTRGLLNHLSDPGQALPAARELGERIAANAPLALAASKQTLVSVRDWTS
                     SEEWQRQAELADPIFGSEDALEGARAFAEKRSPHWQGC"
BASE COUNT          730 a         1711 c         1730 g          705 t
ORIGIN      
        1 gaagttgagt tccacctgct cggccgcttc gaggctgccg ttaccggaga cggtcgactg
       61 cacgaccccg cgcccgacct cggcggtccg ctgctcggcg gtcgcgccgc tggaggagga
      121 gccgatcgag gtgatcgcga tcgcgaggac gaccaggcag aggaggccca gcgcccagtt
      181 gagccagccg ccgggcagcg accgccgctt gcctcctttg gcggcttcgg gaccgcgggt
      241 gctcgcgtgc ccggactcga ggccggcgac gatgctgcga aaacggtggc ggagcttcag
      301 ggctggtggc atgacgaagg atcaaacccg gcgctcctta ggccactcaa agaggaagcg
      361 aagaactgga taagaaaccg atcccgccta ttcgtcgagc gggaagcgca ggatgagcac
      421 ggcgccgcct ccgggcgcgt tcgccgcctc gatcgcggcc ccgtgggcct cggcggtctg
      481 acgcacgatc gccaggccca ggcccgagcc gcgctggctg cgcgccgcgc gggcacggta
      541 gaagcggtcg aagacgaacg gcaggtcctc gggggcgaag ccgggcccgt gatcgcgcac
      601 cctcagcacc ccggccgcga gggtgacctc cacggtcgaa ccgtccgggc tccacttggc
      661 ggcgttgtcg agcaggttgt ggacggcccg cgccagccgc tcgggaacgc cgcgaatcgc
      721 acacggttcg agctgcacgt cgaaggtcag cccgggggcg tggcggcgcg ctcgctcgac
      781 ctcgccggcg acgatctcag acagctccac accgtcgagc agcatcggct cgggctcacc
      841 ccttcgcgcc agctcgacca cgtcgtcgac gagcagcgtc agttcgtcga tctgctcgac
      901 cagttcgcgc tccagctctt cccggtcggc tcctcccagc cgctgctcgc cccggagcag
      961 cagctcgacg ttcgctttca agctcgccag gggcgtcctc agctcgtgcg aggcgtcgga
     1021 gaccatctgc cgctgcgcgt cggccgagct ctgcaaggca tcgagggttg cgttgtaggt
     1081 gcgggcgagc cggcccagct cgtcctcgcc cccgtccggc aggcggcggc tgaggtccga
     1141 gccctcgagg atcgactcgg tctcgtcggt gaacctgcgg accggcgcca gcgaggcgcg
     1201 tgagacgagc gtgccgagca cggccgcaag gccgatgccc ccgatcgtga cgatcaccag
     1261 gatcaggatc agccggtcga gaacgctgtc gacttcgtcg agcggccggg cgacctggac
     1321 cgccccgccg ccctcgagcg gcgcggtcag cacccgcagg tgatcgccgt cgacggtctc
     1381 gtcctcgtac gcgtcccccg actcctcccc ggtcgcaacg gcacgcgcct gcggcgacac
     1441 cggcaggcgc tcggcccccg gcggcgcctg cgctttcacg cttccatccg gttccaggaa
     1501 ctggacgaat ccggcagcgc ctccgaagcg ggcctggggc ggctcgggca ggtcctgccg
     1561 gtcgggaggc gcctgcagcg gaccctccgt ttcgacgatc agctgaacgc cctcagcccg
     1621 ttcgcgcagg gcggaatcga cctcgccgcg cagctgcgag cggaccgcca ggtacgcggc
     1681 cagcgaggcc gtggcgatcg cgatcgccac ggcgaccgcg gcggcgacgg cgaggcggcg
     1741 gcggatcggc atcagcgcgg gcgccgcagg acgtagccga ccccgcgcac ggtctggatc
     1801 agcgacggcg cccctgcttc gtcgagtttg cgacgcaggt agccgacgta gacgccgagc
     1861 gagttcgaag cggcaccgaa gtcatagccc cagacctgct cgttgatcgt cgtccgcgag
     1921 agcacccggc cctggtggcg catcagcagg tgcaggaggg agaactcggt gcgggtcagg
     1981 tcgaccggcc gggttccgac cttgacctcg tagcggacgg gatcgagcag gattccctcg
     2041 tagcgcagcg gctgctcgtc gtcgccggag gcttcggtcc ggcgcagcag cgccttcagc
     2101 cgcgccagca gctcctccag ggcgaagggc ttgacgagat agtcgtcggc tcccgcttcg
     2161 aggccggcga cccggtcggc gaccgtctcg cgcgcggtca gcatcaggat cgggatccgc
     2221 tccccggcag cgcgcagccg cttgcacacg tcgaccccgc tgaggcccgg catcagcacg
     2281 tcgagcacca tcgcgtccca gctgcgctct ttgagcagct cgatcgcctc ctcgccgctg
     2341 gcggcgtcgg tgactccgta tccctcaagc gccagtgccc tgcgcagggc ggctcgcagc
     2401 gggtactcgt cgtcgacgat cagcacctcc atgacagagc cgagcatcgc agccggaatc
     2461 gaagagaaac gtaagaatcg gcggggccgt cgcgcggtcg acccacggtc ggcagggccg
     2521 gagtgtcagt ccggcacgtc ggcgctcgca cggtcgcgtg cgagaccagc gccagtcgaa
     2581 gccagcgccg cagccgggtc gggatcgctg cgacgagggg accgagaatg cggattcgcg
     2641 ccaagcggat tcgctgcggg ggtaccaccg gcggcgacgg aggcccgccg gggccgtgaa
     2701 cgcccagcag cgcaaccgca gcgaccacgg cgacgacccg gttgcgccaa cgggctgccg
     2761 tcgggacctg caccgccatc tcggttctgc gctctgccat gggtcgattc tgcgcccgca
     2821 ggctgagcgc cgcctggcct ctggctgaga ggaacgtgag agaacgccgg aacggaccga
     2881 gcggaggaga gtgcggtgtt cggcgcccgc ccagggaacg tcgtggggag cgaggtcaga
     2941 ctagtgcgac gggacggatc atcgatccgg tcgccccacg gatcttgcag ggaagcgcca
     3001 cgaagaggaa ctcgcgcacc ttgtcggcgg cgagctgctc catgttcagc atctccatga
     3061 tgtagatgcc gttttcgacc agcaggacgg tgtggacggc gtgcgggttc tccgggtcgg
     3121 cgcaggggcc ctgctcgagc gcctcggtgt cgccggcgac ggcaacggcg ccctgctcga
     3181 ccagccagat cgcggcgccc ttgttgatcc ccggcccggc gtgttcggcc atccgctcgg
     3241 catcggggta gagaccgacg tagccggtcc ggatcaggac cgtgtcgccc cggcgcagct
     3301 cggtgccctg cgcggcggcc acctcctgca gctcctcggc ggagactccc tcggctttcg
     3361 gcagcaccgg gacaccgcgg tggccggcga cgtcgagcag cacgccgcgg gtgatgatcg
     3421 tggggatcgc ggcggcgtcg cagaccaggg ggccgaagtc gccgagcttc tcggcggcct
     3481 tgttgccgcc gaaccagtgg ttgtcctcgc cgacggtgac gtgcgcgagc gcgtcgatgt
     3541 gggttccgga gtgaacggtc ccgacgacga ggtcgctgat gaaggccatg ttctcgccgt
     3601 tctttgagtt cagccactcc tgatcgccct ggttttggat gccctgcggc gtgcggaagg
     3661 cgatgatctc catctgcgga tgggcggggt gcagcggcat cccgcggaag cgttcgacct
     3721 cgaggtcgta gaccgttccg ctctggacga gcgacaacgc cgccaagagg tcggagctgc
     3781 cgagctgctt gatcgggcct tgctcctcga ggcgcgaatc ggttctcatc ccactctcct
     3841 tacctggtgt tcttcccatt cagcgaccga gcgctcgctc gggtcgagac tatcgccttt
     3901 gccagttgcg ggtcaaggac aggcaagcta gtcccgagca tccgctcggt aatctgccga
     3961 caccaggcca gccccgaaac ccgaggtgga aagatgagcc agccagaaag cgtggtcagt
     4021 gcccgtgagg gcaagctgct gatcatcacg atcaaccgcc cccaggcacg gaacgccgtc
     4081 gacctctcgg tcgccgaggg cctcgaggcc gcggtcgccg agctcgacgc cgactcggat
     4141 ctggcggtcg gcatagtgac cggcgccggt gggacctttt gcgccggcat ggacctgaag
     4201 gcgttcgcgc gcggcgagcg gccctacacc gagaatggcg gcttcgccgg catcgtcgag
     4261 cgacctcctc gcaagatcct gatcgcggca atcgagggat acgcactcgc cggcggcctg
     4321 gagatcgcgc tctcctgtga cctgctcgtc gccgctcggg acgcgaagct gggcatcccc
     4381 gaggtcggcg tggggctgat cgcggccggc ggggcgctgc tgcggctgcc gagccggatt
     4441 ccctaccacc tggcgatgga gatggcggtg accggcgagc cgatcaccgc cgagcaagct
     4501 cacactcgcg gcctcctcaa ccacctcagc gaccccggcc aggccctccc cgcggcgcgc
     4561 gagctgggcg agcggatcgc ggccaacgcg ccgctcgcgc tcgcggccag caagcagacc
     4621 ctggtctcgg ttcgcgactg gacgagctcg gaagagtggc agcgccaagc ggagctggcc
     4681 gacccgatct tcggctccga ggacgcgctc gagggagccc gcgccttcgc cgagaagcgc
     4741 tcgccgcact ggcagggctg ctaggcctcg atcgcgccga ggccgaggag ctccgcgaag
     4801 cgctcgcggg cctgctcgcg ccggcgcggc aggtgttcgg tcgggactcc gtcggccggc
     4861 gcctcgtagt cgcgca
//
LOCUS       DASRIG010000252         6794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_123627, whole genome shotgun sequence.
ACCESSION   DASRIG010000252 DASRIG010000000
VERSION     DASRIG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123627"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFB52_06860"
     CDS             <1..353
                     /locus_tag="VFB52_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06090.1"
                     /translation="GYPSVVNYVAAKHGVVGLTRALAIELGPHRIRVNNIAPTQVSTD
                     MIHNEAIYKLFVPEVEDPSREQFAEVSQAMHLLPTPWVESIDVSNALLFLVSEEARFI
                     TAASIPVDAGVTQH"
     gene            386..1267
                     /locus_tag="VFB52_06865"
     CDS             386..1267
                     /locus_tag="VFB52_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO06091.1"
                     /translation="MVEFLTTLRLAPGEDAEALFAAQGEAMLARPWPEVGALHKRLAI
                     PGGGEGLDADLVLPAGGEGWPLLLYIHGGGWEAGSPGTHRRVAHEFAARGFATLVPRY
                     RLGPADRHPAQLDDLDAAIAWAQDQLGDYGGDATRLVLAGDSAGAHLAAALAVRRRLA
                     GRDDVGAAVLLTGIFEYHDGLHLVGPHGHDEASQPLLDPAEFEALREDPVVNPLLGAD
                     HLPPAFLGAGSADPFADHSRRLAESLAAAAVPATLDWGEGLPHLWYLLPGLPQSAAGL
                     DRAAAFAHDSLEERDAN"
     gene            1257..1775
                     /locus_tag="VFB52_06870"
     CDS             1257..1775
                     /locus_tag="VFB52_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06092.1"
                     /translation="MPTEMPAGLVSHLIHTCIVCSDFERSLDFYTRVLGAQVGGRVSS
                     GEAGPLSEAMGAPGRRRWRGAMLYWGDPSRTSLVDLIAYDEGSDGEASQPDRSATDLG
                     FARLCLRVTDVEAAARHLRDNDVTLVSDPLEITIEGRLHRVLFFRDPDGTLLEFSERA
                     GPSHAERDYSGE"
     gene            complement(1776..2414)
                     /locus_tag="VFB52_06875"
     CDS             complement(1776..2414)
                     /locus_tag="VFB52_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06093.1"
                     /translation="MAHSAQAPKKPRKRLSAEERKRSILEAAREQFAETGDIGGTTTK
                     MIAARAGVSEAIIYRYFESKEDLYIEAVVEPLRDIVEKTAAAVEDYPEQVTEDQRTAR
                     ARNFYAETIGSMTESLPLFGLVLFGEPATARKFYERCWQPSLDRLSNAWRTYYEENGV
                     KDYPDTDVAARITFGACLMYALDARYGDPLNRREIAGLLAEAGFDGMWLRGT"
     gene            2478..4007
                     /locus_tag="VFB52_06880"
     CDS             2478..4007
                     /locus_tag="VFB52_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06094.1"
                     /translation="MPAAGIASVIRKRASSAVPPGKVALIAGERRVTFGELDEASDRI
                     ASGLKARGFKRGERLAVLGDNTPDWLEVFFAAAKLGGVVVPISHLLKPPEILFQIEDS
                     GATWAFVEAKLLADKFLPIRERAGTELFAINGSAPDVAPGLDELRATAQVTIIAADGP
                     ALADLIVLQYTSGTTGSPKAAMHSHQALLTQALHQIVDSGLSEDDVYLVVPAFSWASG
                     LHYMTLSSFYLGATVALHPLGGFDPDQLCAAIERHRVTRVVMVPTVLRRLVPSGAARR
                     HDLSSLKTVLTGSEPVPVALLEEFAEQLPGVGILQGYGMSEFPISITMLKPEHAIAKV
                     GSAGKATIATELRVVDEAGADRPPGEEGEIVLHSPATMSGYWQRPEETAAALVDGWLH
                     TGDLGYLDEDGFLYIVGRKKDMIISGGLNVYPAEIEKVVAAHPSVRECAVVGTADENF
                     GEVGLAHVVAEEGAPLDLDQLRAYAAEQLSTYKVPKHWVLREQPLPRNPAGKVQKHLL
                     S"
     gene            4042..5118
                     /locus_tag="VFB52_06885"
     CDS             4042..5118
                     /locus_tag="VFB52_06885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO06095.1"
                     /translation="MKVGLNSLMQNIGAPETDGEVVAAELDLALRAEGLGFDSVWAVE
                     HHFSGYSIQPNPLLWLTYLAARTETIELGSAVIVLPWHNPARVAGEISMLDHLCGGRF
                     VAGIGRGLGRLEFEGLGAPMDSSRAHFYEGTEMIAAAMESGVLAYEGEAIEQPTRSLR
                     PHPRASLHGRLYCSAVSPESAAAAAKLGAGILINPQKPWPKIAEDMAAYREEFRRHHD
                     GEAPPPIASCQVYCHEDAAYAEEVARREMGRYYASVMAHYEMTGDHFDGTKGYEYYAR
                     LSEQLQNLEQSDIVDRYLKTQVFGDPEQCIDQIRTIVEVTGSDHFVGLFRYSEIDGDA
                     ALASAELFAREVIPAVKALEPAQV"
     gene            5138..5809
                     /gene="ribB"
                     /locus_tag="VFB52_06890"
     CDS             5138..5809
                     /gene="ribB"
                     /locus_tag="VFB52_06890"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZO06096.1"
                     /translation="MTASKSFNEEVRLDSIEEAVEQLGRGGMAVVVDDPSRENEGDLV
                     MAASFVTPQAVNFMATEARGLICVAMRRQRLEELGLPPMVARSDDPFGTAFHVAVDIA
                     DRSSTGISAADRARTIAALGDPHSRAEDFVCPGHVFPLAARDGGVLARRGHTEASVDL
                     CALAGLPEAGVICEIANDDGEMARLPELRRFADRHGLPLVAIAELRAELRARTKPARP
                     HEVLL"
     gene            5806..>6794
                     /locus_tag="VFB52_06895"
     CDS             5806..>6794
                     /locus_tag="VFB52_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06097.1"
                     /translation="MSLNLALVLADSARAVPDEPALVHEGETITFAELDRASARLAGW
                     LWARGLGPGDRVALMLPNVPEFAVAYYGVLRAGMVVVPMNPQLRQREVDYFLQDSGAS
                     LLIADQGPFAEAAGAEPFEEVVRRDPSEPAVFLYTSGTTGKPKAAVLSHSNLLWNAEV
                     TSETFGIQAGDRLLGTLPLFHSFGQSCVLNGAVRRRATVALLPRFAPHDALALIERES
                     ITHFYGVPTMFVALLQALEEAPADVSSLRLCLSGGASIARETLDAFQRRFDVRVLEGY
                     GLSETAPACCFSRVDTPAKPGTIGRPLWGVEMRVAGEDGAALPSGEVGEIHVR"
BASE COUNT          958 a         2521 c         2316 g          999 t
ORIGIN      
        1 agggctaccc gagcgtggtc aactacgtgg ccgcaaagca cggggtggtc gggctgaccc
       61 gcgctctggc gatcgagctg ggaccgcacc ggatccgcgt caacaacatc gccccgaccc
      121 aggtctcgac cgacatgatc cacaacgagg cgatctacaa gctgttcgtg cccgaggtcg
      181 aggacccgag ccgcgagcag ttcgccgagg tctcgcaggc gatgcacctg ctgccgacgc
      241 cctgggtcga gtcgatcgac gtctccaacg cgctgctctt cctcgtctcc gaggaggccc
      301 gcttcatcac cgcggcgagc atcccggtcg acgccggcgt cacccagcat tagtggagca
      361 actcccgcca cagccgccgc gggaggtggt cgagttcctg accacgctgc ggctggcgcc
      421 tggcgaggac gcggaagcgc tgttcgcggc gcagggcgag gcgatgctgg cgcggccctg
      481 gcccgaggtc ggagccctgc acaagcgcct cgcgattccc ggcggcgggg aggggctcga
      541 cgccgacctg gtgctgccgg cgggcggcga gggctggccg ctgctgctct acatacatgg
      601 gggaggctgg gaagccggca gccccgggac gcaccggcgg gtcgcccacg agttcgccgc
      661 ccgtggcttc gcgaccctgg tcccgcgcta ccggctcggc cccgccgatc gtcaccccgc
      721 ccagctcgac gacctcgacg cggcgatcgc ctgggcccag gatcagctcg gcgactacgg
      781 cggcgacgcc acgcggctgg tcctcgccgg cgactcggcc ggcgcccatc tggccgctgc
      841 gctggcggtc cgccgccggc tcgccgggcg cgacgacgtc ggcgccgccg tcctgctgac
      901 cgggatcttc gagtaccacg acgggctgca cctggtcggt ccccacggcc acgacgaggc
      961 gagccagccg ctgctcgacc cggccgagtt cgaggcgctg cgcgaggacc cggtcgtcaa
     1021 ccccctgctc ggcgccgacc acctgccgcc ggccttcctc ggcgccggct ccgcggaccc
     1081 gttcgccgac cactcgcgcc ggctcgccga gtcgctggcc gccgccgccg tccccgccac
     1141 gctcgactgg ggtgagggcc tgccgcacct ctggtacctg ctgcccggcc tgcctcagag
     1201 cgccgccggc ctcgaccgcg ccgccgcctt cgcccacgac agcctggagg aacgagatgc
     1261 caactgagat gcccgcgggc ctggtcagcc acctgatcca cacctgcatc gtctgctcgg
     1321 acttcgagcg ctcgctcgac ttctacaccc gcgtgctcgg cgcccaggtc ggcggccgcg
     1381 tctcctctgg tgaggcggga cccctgtcgg aggcgatggg cgcaccgggc cgccgccgct
     1441 ggcgcggcgc gatgctctac tggggcgacc ccagccgcac ctccctggtc gacctgatcg
     1501 cctacgacga aggcagcgac ggcgaggcca gccagcccga ccgcagcgcc accgacctcg
     1561 gcttcgcccg cctctgcctg cgcgtcaccg acgtcgaggc cgccgcccgg cacctgcgcg
     1621 acaacgacgt caccctcgtc tccgaccccc tggagatcac gatcgagggc cgcctgcacc
     1681 gcgtcctctt cttccgcgac cccgacggca ccctgctcga gttctccgag cgcgccggcc
     1741 catcccacgc cgagcgcgac tactcgggcg agtagctagg tcccgcgcag ccacatgccg
     1801 tcgaagccgg cctcggcgag gaggccggcg atctcgcggc ggttcagcgg gtcgccgtag
     1861 cgggcgtcga gcgcgtacat caggcaggcg ccgaaggtga tgcgggcggc gacgtcggtg
     1921 tcggggtagt ccttgacccc gttctcctcg tagtaggtgc gccaggcgtt gctgaggcgg
     1981 tcgagcgagg gctgccagca gcgctcgtag aacttgcgcg cggtcgccgg ctcgccgaac
     2041 agaacgaggc cgaagagggg gagggactcg gtcatcgagc cgatcgtctc cgcgtagaag
     2101 ttgcgggcgc gggcggtgcg ctggtcctcg gtgacctgct cgggatagtc ctcgaccgcg
     2161 gcggcggtct tctcgacgat gtcgcgcagg ggctctacca ctgcctcgat gtagaggtcc
     2221 tccttgctct cgaagtagcg gtagatgatc gcctcgctga ccccggcccg ggcggcgatc
     2281 attttggtcg tggtgccgcc gatgtcgccg gtctcggcga actgctcgcg ggcggcctcg
     2341 aggatcgagc gcttgcgctc ctccgcgctc aggcgtttgc ggggtttttt cggcgcctga
     2401 gctgaatgag ccatcactta caaccctaga cgaccatctt gaaagtgtgc acttacttag
     2461 gatagattcg acccaccatg cccgccgccg gtatcgccag cgtgatccgc aagcgcgcgt
     2521 cctcagctgt gccgccgggc aaagtcgcgc tgatcgcggg cgagcgccgg gtcaccttcg
     2581 gcgagctcga cgaggccagc gaccggatcg cctccgggct gaaggcgcgc ggcttcaagc
     2641 gcggcgaacg gctcgcggtg ctgggcgaca acacccccga ctggctcgag gtcttcttcg
     2701 ccgccgccaa gctgggcggg gtcgtcgtcc cgatcagcca cctgctgaag ccgccggaga
     2761 tcctcttcca gatcgaggac tcgggcgcca cctgggcttt cgtcgaagca aagctgctgg
     2821 ctgacaagtt cctcccgatc cgggagcggg ccggcaccga gctgttcgcg atcaacggct
     2881 ccgcgccgga cgtcgccccc ggcctcgacg agctgcgggc gaccgcgcag gtgacgatca
     2941 tcgccgccga cggcccggcg ctcgccgacc tgatcgtgct ccagtacacc tcgggcacca
     3001 ccggcagccc gaaggcggcg atgcactccc accaggcgct gctgacccag gcgctgcacc
     3061 agatcgtcga cagcggcctc agcgaggacg acgtctacct ggtcgtgccc gccttctcct
     3121 gggcctcggg cctgcactac atgacccttt ccagcttcta cctcggggcg accgtcgccc
     3181 tgcacccgct cggagggttc gaccccgacc agctctgcgc ggcgatcgag cgccaccggg
     3241 tcacccgggt ggtgatggtg ccgaccgtct tgcgccgcct cgtcccctcc ggtgccgccc
     3301 ggcgccacga cctctccagc ctgaaaacgg tgctgaccgg atccgagccg gtgccggtgg
     3361 cgctgctcga ggagttcgcc gagcagctgc cgggggtcgg catcctgcag ggctacggga
     3421 tgtcggagtt cccgatctcg atcacgatgc tcaagcccga gcacgcgatc gccaaggtcg
     3481 gctcggccgg caaggcgacg attgccaccg agctgcgcgt ggtcgacgag gccggtgcgg
     3541 atcgcccgcc cggggaggag ggcgagatcg tcctgcactc gccggcgacg atgagcggct
     3601 actggcagcg ccccgaggag accgcggcgg cgctggtcga cggctggctg cacaccggcg
     3661 acctcggcta cctcgacgag gacggcttcc tctacatcgt cggccgcaag aaggacatga
     3721 tcatctccgg cggtctcaac gtctacccgg ccgagatcga gaaggtcgtg gccgcccacc
     3781 cgagcgtgcg cgaatgcgcc gtagtcggca ccgccgacga gaacttcggc gaggtcgggc
     3841 tcgcccacgt ggtcgccgag gaaggggccc cgctcgacct cgaccagctc cgtgcctacg
     3901 cggccgaaca gctctccacc tacaaggtcc ccaagcactg ggttctgcgc gagcagccgc
     3961 tgccgcgcaa ccccgctggc aaggtgcaga agcacctgct aagttagtga ccattcacta
     4021 acttacgtcg gaggtttcgg gatgaaggtc gggctcaact cgctcatgca gaacatcggt
     4081 gcgccggaga ccgacggcga ggtggtcgcc gccgagctcg acctggcgct gcgggcggag
     4141 gggttggggt tcgactcggt ctgggcggtg gagcaccact tcagcggcta ctcgatccag
     4201 cccaacccgc tgctctggct cacctacctg gcagcgcgga ccgagacgat cgagctcggc
     4261 tcggcggtga tcgtgctgcc ctggcacaac ccggcccggg tggcggggga aatcagcatg
     4321 ctcgaccacc tctgcggcgg ccgtttcgtc gccggcatcg ggcgcgggct cggccggctg
     4381 gagttcgaag gtctgggcgc gccgatggac agctcgcggg cgcacttcta cgagggcacc
     4441 gagatgatcg cggcggcgat ggagagcggc gtgctcgcct acgagggcga ggcgatcgag
     4501 cagccgaccc gctcgctgcg cccccacccg cgggcctcgc tgcacggccg cctctactgc
     4561 tcggcggtct cgcccgagtc ggccgccgcc gccgccaagc tcggcgccgg gatcctgatc
     4621 aacccgcaga agccgtggcc gaagatcgcc gaggacatgg ccgcctaccg cgaggagttc
     4681 cgccgccacc acgacggcga ggcgccgccg ccgatcgcct cctgtcaggt ctactgccac
     4741 gaggacgccg cgtatgccga ggaggtggcg cgccgcgaga tgggccgcta ctacgcctcg
     4801 gtgatggcgc actacgagat gaccggcgac cacttcgacg ggaccaaggg ctacgagtac
     4861 tacgcgcggc tctccgagca gctgcagaac ttggagcagt ccgacatcgt cgaccgctac
     4921 ctgaagaccc aggtcttcgg cgatcccgag cagtgcatcg accagatccg cacgatcgtc
     4981 gaggtcaccg gcagcgacca cttcgtcggc ctctttcgct actcggagat cgacggcgac
     5041 gcggcgctgg ccagcgccga gctgttcgcc cgcgaggtga tcccggcggt gaaagcgctc
     5101 gagcccgcgc aggtctgagg gggtttaagc tatgcagatg acagcctcta aatcatttaa
     5161 cgaagaggtt aggctggact cgatcgagga ggcggtcgag cagctcggcc gcggcggcat
     5221 ggccgtggtc gtcgacgacc ccagccgcga gaacgagggc gacctggtga tggcggccag
     5281 cttcgtcacc ccgcaggcgg tcaacttcat ggcgaccgag gcccgcggcc tgatctgcgt
     5341 ggcgatgcgc cgccagcggc tcgaagagct cggcctgccg ccgatggttg cccgcagcga
     5401 cgaccccttc ggcaccgcct tccatgttgc ggtcgacatc gcagaccgct cgagcaccgg
     5461 gatctcggcc gccgaccggg cgcgcactat cgccgccctc ggcgacccgc acagccgggc
     5521 cgaggacttc gtctgcccgg gccacgtctt cccgctcgcg gcccgcgacg gcggcgtgct
     5581 cgcccgccgc ggccacaccg aggcctcggt cgacctctgc gcgctagccg gcctccccga
     5641 agccggggtg atctgcgaga tcgccaacga cgatggcgag atggcgcgcc tgccggaact
     5701 gcgccgcttc gccgaccgcc acggcctgcc cctggtcgcg atcgccgagc tgcgggccga
     5761 gctgcgcgcc cggaccaagc ccgcccgacc ccacgaggtc ctcttgtgag cctcaacctc
     5821 gccctggtgc tggccgacag cgcccgcgcc gtgccggatg agccggcgct ggtgcacgag
     5881 ggggagacga tcaccttcgc cgagctggac cgggcctcgg cccggctcgc cggctggctc
     5941 tgggcgcgcg ggctcggccc cggcgaccgg gtcgccctga tgctccccaa cgtgcccgag
     6001 ttcgcggtcg cctactacgg ggtcctgcgc gccggcatgg tcgtcgtgcc gatgaacccg
     6061 cagctgcgcc agcgcgaggt cgattacttc ctccaggact ccggcgcctc cctgctgatc
     6121 gccgaccagg gccccttcgc cgaagccgcc ggcgccgagc cgttcgagga ggtcgtccgc
     6181 cgcgacccct ccgagcccgc cgtcttcctc tacacctcgg gcaccaccgg caagccgaag
     6241 gcggcggtgc tctcccactc gaacctgctc tggaacgccg aggtcaccag tgagaccttc
     6301 ggcattcagg ccggcgaccg tctgctcgga actctccccc tgtttcacag cttcgggcag
     6361 agctgcgtcc tcaacggcgc ggtccgtcgc cgggcgaccg tggccctcct cccgcggttc
     6421 gcaccccacg atgctctcgc gctgatcgag cgcgagagca tcacccactt ctacggcgtg
     6481 ccgacgatgt tcgtggcgct gctgcaggcg ctcgaagagg cgccggccga tgtctcctcg
     6541 ctgcgcctct gcctctccgg cggcgcctcg atcgcgcgcg agacgctcga tgccttccag
     6601 cgccgcttcg acgtgcgcgt gctcgagggc tacgggctct cggagacggc gcccgcctgc
     6661 tgcttctcgc gggtcgacac gccggccaag cccggcacga tcggccgccc gctctggggc
     6721 gtggagatgc gggtcgccgg cgaggacggc gcggccctgc cgagcggcga ggtcggcgag
     6781 atccacgtgc gcag
//
LOCUS       DASRIG010000253         6985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2196791, whole genome shotgun sequence.
ACCESSION   DASRIG010000253 DASRIG010000000
VERSION     DASRIG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196791"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..793
                     /locus_tag="VFB52_06900"
     CDS             155..793
                     /locus_tag="VFB52_06900"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZO06098.1"
                     /translation="MSEANPFGRGRLRRRFGLDRSAPPPKVTRKGEPLNPWTLPNLVG
                     LVRFLAIPVFLLMAYNTEDGTSFAAAMVFWAIAAGDYLDGFLARATGQYSRLGTLLDP
                     FVDRLTVLAGAVVCWSFELLPRWLLLLLLAREVAMLVLAQYGLRHGVDIEVNWPGRIA
                     VFPTMGGIWLALFAPGWVATALVIWGTAMAILATVLYARTGMRQARAARASG"
     gene            933..1199
                     /locus_tag="VFB52_06905"
     CDS             933..1199
                     /locus_tag="VFB52_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06099.1"
                     /translation="MEDTFPDLGSLSDKELKDLIASLTEEENEISYKRRVLHGRIDIL
                     RAELVNRLRQKREHGDSLISGDDVEQLTEILAGKVPPPEGDSAA"
     gene            1215..1691
                     /locus_tag="VFB52_06910"
     CDS             1215..1691
                     /locus_tag="VFB52_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO06100.1"
                     /translation="MSAVHCPECGFANPEGANYCQKCGAYLAGPKEEGEDPTTMTYRV
                     DETGDMQPVAIDDVVEESGAALVIRAGGGRAGESFSISEDRVSIGRSPDAGVFLDDVT
                     VSRNHALLVKRRDGLYIDDLGSLNGTYVNRRRIESHRLADGDEIQVGKYKLSYLER"
     gene            1696..2442
                     /locus_tag="VFB52_06915"
     CDS             1696..2442
                     /locus_tag="VFB52_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO06101.1"
                     /translation="MAVSESTARTPDGPSRPRKALTIGAVCKILQSEFDDVSISKIRY
                     LEDQKLLSPRRTSGGYRLYSQSDVERLRTILRLQRDEFLPLRVIRQELASGGDLVLGG
                     DGDKKPTSGAVRRAILVDTSRTYLSIEEIVEETGAREELIRELENFGIVQPEKREGKT
                     VYDETDREIVRAANELSRVGVGARNLRVFRSSADREAQLLEALLGPSLRSRNPDRRKE
                     ALQSLESLAATVSHLKHLLLVRDLRRLAGD"
     gene            2450..2920
                     /locus_tag="VFB52_06920"
     CDS             2450..2920
                     /locus_tag="VFB52_06920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO06102.1"
                     /translation="MPRVSRRRTIEAPVDEVWKLVSDPYNLPRWWPRTSRVENVDRRD
                     GGRRSQWTQVLETAEGRGVRADYRCLSSATNERYVWEQELEGTPFAKHLKRAEFAVEL
                     RGDGESTEVELSSSQSTRGLSKLGSPMLRNAQTKILDAALDGIEEALSTKSASG"
     gene            2938..4563
                     /locus_tag="VFB52_06925"
     CDS             2938..4563
                     /locus_tag="VFB52_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO06103.1"
                     /translation="MEPRRDTKWWGWGDPGIAPELDDAALGVLRERIGALEPWPLNAA
                     DLGEFVMLNPQPLPPKLTEVVGPERIFTSKEDRLRHASGRGYADLARMRIGKLEDAPD
                     AVVLPASAEQLRALLQVCSEEGIAVVPFGGGTSVVGGVEPLRGKHQALISLDLADLRE
                     VSVDQRSLTARLGAGLRGPEAEAALGEQGVTLGHFPQSFQYATIGGFAATRSAGQASS
                     GYGRFDALVSSVRLVAPAGEMETLETPHTAAGPALRELIVGSEGVLGVIPDVTVRVRP
                     APAKKRYEAWMAESFEAGAEIVRGLAQGSGLPEIIRVSDEEETEGSLAVSGPRGFAAK
                     AFDGYLGVRKRRGGALMIVGYEGDEESTSRRRALTVRGLRAGGAAYLGQSAGGSWEHG
                     RYNGPYLRDTLMEMGAMVETLETSHTWTRLGELHDAVAAAIHDTLAGGGTPGLVWCHL
                     SHAYADGASLYFTFISRRRAGAELEQWREVKEAACEAIVATGGTITHHHAVGRDHAPY
                     MKAEVGETGIDVLRAVKDQLDPAWIMNPGKLLP"
     gene            complement(4579..5556)
                     /locus_tag="VFB52_06930"
     CDS             complement(4579..5556)
                     /locus_tag="VFB52_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO06104.1"
                     /translation="MPLSDDQKAMLRLLARDQSYEDIAALMGLSVDEVVARAQGAVAQ
                     LEAEGIPAPTLPEASGGAAVTPPDPVEKAPEPPKAEEKPAAKEPEPAPAPPAKKPPPA
                     AATPPPSKPSAPKKPRDRGMLLAIGGGVFAVVVIIVLLVLLISNGDGDDSGNSTANGG
                     DDTAIAENGGQTQKPTTAQLVAVDGSSASGTATFGRVEESLGLGIRASGLEQLAKGSI
                     YMVWLAESPQKMLPLTAVVANKAGRINASYQVPTEAVIYLATGEFDDLVITRANNKEL
                     RASLEAANKEKNFPGYTGDAVLEGQVIGPIIGAAKRLEERQKERKEEAE"
     gene            5644..6051
                     /locus_tag="VFB52_06935"
     CDS             5644..6051
                     /locus_tag="VFB52_06935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZO06105.1"
                     /translation="MDRDDTGEIRSPFDLLVGSEVLSDDPDDARLRVQVRDELRQPVG
                     LLHGGVLSTLVESICSRGTAMVVWPEGNAAMGQSISVNFIRPVTEGHAEVRARARHRG
                     RTTWVWDCEVLDAQERLCATAMMTIAVRPLPKS"
     gene            complement(6048..>6985)
                     /gene="nadE"
                     /locus_tag="VFB52_06940"
     CDS             complement(6048..>6985)
                     /gene="nadE"
                     /locus_tag="VFB52_06940"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZO06106.1"
                     /translation="SEPGPLAEPLADVDEVYEALTLGLRDYVEKNGFRHVGVALSGGI
                     DSALVALLAADAVGAERLSCVVMPSPHSSTETQQDARNIAANLGCELIEIEIQPMMDG
                     YERALTGLLNPGEAPEPGEENQPPHDPARPSEPDLAAENIQARIRGNMMMALSNRHGW
                     LVLTTGNKSEMSVGYATLYGDMAGGFAVIKDVPKELVYALTRRRNERAGRELVPASVL
                     ERAPSAELRPDQLDSDSLPPYDLLDRILEAYVERDQGREEMVAAGMPDDVVDEVIRMV
                     DRSEYKRRQAAPGIRITPRAFGKDRRLPITNRFGG"
BASE COUNT         1002 a         2443 c         2472 g         1068 t
ORIGIN      
        1 gagctgctcg agaagctcaa ggtcacggtc cgctgcatcc ccaccgacca gcggctcgag
       61 cccggcagca tctgcgtgct gaccggcaaa ccggccgtcg tcgaggcggt cttcgccaag
      121 gcctactgag ggccgtccgc gccgggggag cgggatgagc gaggccaacc cattcggacg
      181 cggccgcctg cgccgccgct tcgggctcga ccgcagcgcg ccgccgccga aagtgacccg
      241 caagggcgaa ccgctgaacc cgtggacgct gcccaacctg gtcgggctgg tgcgattcct
      301 ggcgatcccg gttttcctct tgatggccta caacaccgag gacgggacct cgttcgccgc
      361 cgcgatggtc ttctgggcga tcgcggccgg cgactacctc gatggcttcc tcgcccgcgc
      421 gaccggccag tacagccgcc tcgggacgct cctcgacccg ttcgtcgatc gcctcaccgt
      481 gctcgccggc gcggtcgtct gctggagctt cgagctgctg ccgcgctggc tcctgctcct
      541 gctcctggcg cgcgaggtcg cgatgctcgt cctcgcccag tacgggctgc gccacggggt
      601 cgacatcgag gtcaactggc ccggccggat cgccgtcttc ccgaccatgg gcgggatctg
      661 gctggcgctg ttcgcgcccg gctgggtggc gacggccctg gtgatctggg gcacggcgat
      721 ggcgatcctc gccacggtgc tctacgcccg cacggggatg cggcaggccc gcgctgcgcg
      781 agcgtcgggc tgaccccggc ggcgcctgca agctcgccgc caaatctgcc tgaaccttca
      841 acctgcggtt gaaccttgcc gcggtctcga cctataatcg ctgctcgcgc aaacggaggc
      901 gtcagcgccc cctctcgagc ttaggagcat tgatggagga cacctttccc gatctggggt
      961 cgctcagcga caaggaactg aaagacctga tcgccagcct gaccgaagag gagaacgaga
     1021 tctcctacaa acggcgcgtc ctgcacggcc gcatcgacat cctgcgggcc gagctggtca
     1081 accgcctgcg ccagaaacgc gagcacggcg actcgctgat ctccggcgac gacgtcgagc
     1141 agctgaccga gatcctcgcc ggcaaggtgc cgcccccgga gggcgactcc gccgcctgag
     1201 cgccggggat tccgatgagc gccgtccact gtcccgagtg cgggttcgcc aaccccgagg
     1261 gtgcgaacta ctgccagaag tgcggtgcct acctggcggg cccgaaggag gagggcgagg
     1321 acccgacgac gatgacctac cgggtcgacg agaccggcga catgcagccg gtcgcgatcg
     1381 acgacgtggt cgaggagtcg ggcgcggcgc tggtgatccg cgctggcggc ggccgcgcgg
     1441 gggagagctt cagcatctcc gaggaccggg tctcgatcgg gcgcagcccc gacgccggcg
     1501 tcttcctcga cgacgtcacc gtctcccgca accacgcgct gctggtcaag cgccgcgacg
     1561 gcctctacat cgacgacctc ggctcgctca acgggaccta cgtgaaccgc cgccggatcg
     1621 agtcgcaccg gctggcggac ggcgacgaga tccaggtcgg caagtacaag ctgagctacc
     1681 tggagcgctg aggcgatggc cgtctccgaa tccacggcgc gcacgccgga cgggccgagc
     1741 cgtccgcgca aggccctgac gatcggcgcc gtctgcaaga tcctgcagag cgagttcgac
     1801 gacgtctcga tctcgaagat ccgctacctc gaggaccaga agctgctcag cccgcgacgc
     1861 acctcgggcg ggtatcgcct ctacagccag tccgacgtcg aacggctgcg cacgatcctg
     1921 cgcctgcaac gcgacgagtt cctgccgctg cgggtgatcc gccaggagct ggcctccggc
     1981 ggcgacctcg tgctcggcgg cgacggcgac aagaagccga ccagcggtgc ggtgcgccgc
     2041 gcgatcctgg tcgacacctc gcgcacgtac ctgtcgatcg aggaaatcgt cgaggagacc
     2101 ggcgcccgcg aggagctgat ccgcgagctg gagaacttcg gaatcgtgca gccggagaaa
     2161 cgcgagggca aaacggtcta cgacgagacc gatcgcgaga tcgtccgcgc cgccaacgaa
     2221 ctctcccggg tcggggtcgg cgcccgcaac ctgcgcgtct tccgctcctc cgcggatcgc
     2281 gaggcgcagc tgctggaagc gctgctggga ccctcgctgc gctcgcgcaa ccccgaccgg
     2341 cgcaaagagg ctctacagag cctcgaaagc ctcgccgcga cggtctccca cctcaagcac
     2401 ctgctgctgg tccgggacct gcgccgcctg gccggcgact gaggccgcga tgccgcgggt
     2461 gagccggcgc cgcacgatcg aggcgcccgt cgacgaggtc tggaagctcg tctccgaccc
     2521 ctacaacctg ccgcgctggt ggccgcgcac ctcgcgggtc gagaacgtcg accgcaggga
     2581 cggcggccgc cgcagccagt ggacgcaggt gctggagacc gccgagggcc gcggcgtgcg
     2641 cgccgactac cgctgcctca gctcggcgac gaacgagcgc tacgtctggg agcaggagct
     2701 cgagggcacg ccgttcgcca agcacctgaa gcgggctgag ttcgcggtcg agctgcgggg
     2761 ggacggcgag tcgaccgagg tcgagctgag ctcctcgcaa agtacccgcg gactttcgaa
     2821 gctcggctcg ccgatgctga gaaacgctca aaccaagatc ctcgacgccg ccctcgacgg
     2881 gatcgaggag gccctttcga ccaagtcagc ctctggttga caaactcgat agccgggatg
     2941 gagccgcgac gcgacacgaa gtggtgggga tggggggatc cggggatcgc ccccgagctc
     3001 gacgacgccg cgctcggcgt cctgcgcgag cgaatcggcg cgctggagcc gtggccgctg
     3061 aacgccgctg atctgggcga gttcgtgatg ctgaacccgc agccgctgcc gcccaagctg
     3121 accgaggtcg tcgggcccga gcggatcttc acttcgaagg aggaccggct gcgccacgcc
     3181 agcggccgcg gctatgccga cctcgcacgg atgcggatcg gcaagctgga ggatgcgccc
     3241 gacgcggtcg tgctgccggc gagcgccgag cagctgcgcg cgctgctgca ggtctgcagc
     3301 gaggagggga tcgcggttgt gcccttcggt ggcggtacca gcgtcgtcgg tggggtcgag
     3361 ccgctgcgcg gcaagcacca agcgctgatc agcctcgacc tggccgacct gcgcgaggtc
     3421 agcgtcgacc agcgctcgct gaccgcgcgg ctcggcgccg ggttgcgcgg tcccgaggcc
     3481 gaggcggccc tgggcgagca gggcgtcacg ctcggccact tcccgcagtc cttccagtac
     3541 gcgacgatcg gcggcttcgc cgcgacccgc tcggccggac aggcctcgag cggctacggg
     3601 cgctttgacg ccctggtcag ctctgtgcgg ctggttgcgc cggccgggga gatggagacc
     3661 ctggaaaccc cgcacacggc cgctgggccg gcgctgcgcg agctgatcgt cggctccgag
     3721 ggcgtgctcg gcgtgatccc cgacgtgacc gtgcgcgtgc ggccggcgcc ggcgaagaag
     3781 cgctacgagg cctggatggc ggagagcttc gaggccgggg cggagatcgt ccgcggcctc
     3841 gcccaggggt cgggcctgcc ggagatcatc cgcgtctccg acgaggagga gaccgaaggc
     3901 tcgctcgccg tctccggccc gcgcggcttc gccgccaagg ctttcgacgg ctacctcggg
     3961 gtgcgcaaac ggcgcggcgg agcgctgatg atcgtcggct acgagggcga cgaggagtcg
     4021 acctcgcggc ggcgggcgct gaccgtgcgc ggcctgcgcg ccggcggtgc cgcctacctc
     4081 ggccagtcgg ccggcggctc ctgggagcac ggccgctaca acggccccta cctgcgcgac
     4141 acgctgatgg agatgggcgc gatggtcgag accctggaga cctcgcacac ctggacccgg
     4201 ctcggcgagc tccacgacgc ggttgccgcc gcgatccacg acacgctcgc cggcgggggc
     4261 acgccggggt tggtctggtg ccatctctcg cacgcctacg ccgacggcgc ctcgctctac
     4321 ttcaccttca tctcccgccg ccgcgccggc gccgagctcg agcagtggcg cgaggtcaag
     4381 gaggccgcct gcgaggcgat cgtcgcgacc ggcggcacga tcacccacca ccacgccgtc
     4441 ggccgcgacc acgcgcccta tatgaaggcc gaggtggggg agaccgggat cgacgtgctg
     4501 cgcgcggtca aggaccagtt ggatccggcc tggatcatga acccgggcaa gctgctgccc
     4561 tgagcgggcg gcgctcgcct attcggcttc ttctttgcgt tctttctggc gttcttcgag
     4621 gcgtttcgcg gccccgatga tcgggccgat gacctggcct tcgagcactg cgtcgccggt
     4681 gtacccgggg aagttttttt ctttgttcgc ggcttcgagc gaggcgcgca gttctttgtt
     4741 gttcgcccgg gtgatcacga gatcgtcgaa ttccccggtc gcgaggtaga tcaccgcttc
     4801 ggttggcacc tggtaggagg cgttgatccg cccggctttg ttggcgacga ccgcggtcag
     4861 cggcagcatt ttctgcggcg actcggccag ccagaccatg tagatgctgc ctttggcgag
     4921 ctgttcgagg cccgaggccc tgatccccag cccgagcgat tcttcgaccc ggccgaaggt
     4981 cgccgtgccg ctggcgctgc tgccgtcaac cgcgaccagc tgcgcggtgg tcggtttctg
     5041 ggtctgtccg ccattttcgg cgattgccgt gtcatcgcca ccgttggcgg tgctgttgcc
     5101 ggagtcgtcg ccatcgccgt tggagatcag caggacgaga aggacgatga tcacgaccac
     5161 ggcgaagact ccgccgccga tcgcgagcag catgcctctg tcgcgtggct tcttgggcgc
     5221 ggaaggtttg gacgggggag gagtagctgc ggctgggggc ggcttcttgg ccggcggcgc
     5281 cggggccggc tcgggctctt tggcggcagg cttctcctcg gcctttggcg gctcgggggc
     5341 cttctcgacc gggtcgggtg gggtcacagc ggcgccaccg gacgcctctg gcagggtcgg
     5401 cgccgggatc ccttccgctt cgagctgggc gactgcgccc tgtgcccgcg ccacgacctc
     5461 gtccacgctc agccccatca gggcggcgat gtcctcgtag ctctggtcgc gcgccagcag
     5521 gcgcagcatc gctttctggt cgtcgctgag cggcatcccc agcggaaccc tacgacgccg
     5581 gggcggcgga ttgccgcccg cctggagagg gtcgcgaaag cttaatttcg tagggtgcgg
     5641 ggaatggacc gcgacgacac gggtgagatc cgctcgccct tcgacctcct ggtcggcagc
     5701 gaagtgctct ccgacgaccc cgacgacgcg cgcctgcggg tgcaggtccg cgacgagctg
     5761 cgccaaccgg tcggccttct gcacggcggc gtcctctcca ccttggtcga gagcatctgc
     5821 tcacggggca ccgcgatggt cgtctggccc gagggcaacg cggcgatggg ccagtcgatc
     5881 agcgtcaact tcatccgccc ggtgaccgag ggccacgccg aagtccgcgc ccgcgcccgc
     5941 caccgcggcc gcaccacctg ggtctgggac tgcgaggtcc tcgacgcgca ggagcgcctc
     6001 tgcgcgacgg cgatgatgac gatcgccgtg cggccgctgc cgaagtccta gccgccgaag
     6061 cggttcgtaa tcgggaggcg gcggtccttg ccgaaggccc gcggggtgat gcggatcccg
     6121 ggcgccgcct ggcggcgctt gtactcggaa cggtcgacca tccggatcac ctcgtcgacg
     6181 acgtcgtcgg gcatcccggc ggcgaccatc tcctcgcggc cctggtcgcg ctcgacgtag
     6241 gcctcgagga tgcggtcgag caggtcgtaa ggcggcagcg agtcactgtc cagctggtcg
     6301 ggccgcagct ccgccgaggg cgcccgctcg agcacgctgg ccgggaccag ctcgcgcccg
     6361 gcgcgctcgt tgcgccgccg cgtcagcgcg tagaccagtt ccttcggcac gtccttgatc
     6421 accgcgaagc cccccgccat gtcgccgtag agggtcgcgt agccgaccga catctcgctc
     6481 ttgttgccgg tggtcaggac cagccagccg tggcggttgg agagcgccat catcatgttg
     6541 ccgcggatcc gcgcctggat gttctcggcg gcgagatccg gctctgaggg ccgcgccgga
     6601 tcgtgcggag gctggttctc ctcgccgggc tcgggcgcct cgcccgggtt gagcagcccg
     6661 gtcagggcgc gctcgtagcc gtccatcatc ggctggatct cgatctcgat cagctcgcat
     6721 cccaggtttg cagcgatatt tctggcgtcc tgctgggttt cggtgctgga atgtggagac
     6781 ggcatcacca cgcagctgag ccgctcggcc ccgaccgcgt cggcggcgag cagcgcgacc
     6841 agcgccgagt cgatcccgcc cgagagggcg acgccgacgt ggcggaaacc gttcttctcg
     6901 acgtagtcgc gcagcccgag ggtgagcgcc tcgtagacct cgtcgacgtc ggccaagggc
     6961 tcggccagcg gccccggctc gctcg
//
LOCUS       DASRIG010000254         9398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_124500, whole genome shotgun sequence.
ACCESSION   DASRIG010000254 DASRIG010000000
VERSION     DASRIG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124500"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFB52_06945"
     CDS             complement(<1..563)
                     /locus_tag="VFB52_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06107.1"
                     /translation="MARTVAKTQAEYEANRRFALALILVLAGVGGVLLIAAAVIFASA
                     ADADESQMVFSGVIPILGTWVGTVLAFYFARDNLKAATDSAVALAKSETAAETDVSAV
                     MIGLAEIVAEDVQDDAAARALSVKALKKKMLDATPPRGRLPIRDAKGAVLYVLHESTA
                     EKFITSPTRPPGDSPTFGELLDLPEFRG"
     gene            complement(653..2302)
                     /locus_tag="VFB52_06950"
     CDS             complement(653..2302)
                     /locus_tag="VFB52_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06108.1"
                     /translation="MESRPLRPTPRFSPRLALLLAALVVVLALPASASAGTIVADNGF
                     RPNPNGFSFQNYGGEEGYANLDASEFERLFGPGVCMPGKGKCVLTPIASTYMKAFNEG
                     SNGGHCFGFATLSELIYKGYLPRFGYSSISGLGRGATTTFSLGIKGNKLLQRSIARAF
                     NFQTLSSINENTLIGTPVQILNTLLQGALNPQEKEIWTFEIFQYGFKAGHAITPYAVE
                     NMGNGIYDVHVYDNNWPDDDTRRLQIDTKKNTWSYYAAINPGFPQAQYEGNAKSKSLR
                     LAPVTPGLGVQPCPFCVGRQGGGSKFNEIRLDGTADQHARLLVVDDKGRKTGSVGNRI
                     VNQIPGAKVLPRSSAGILPLANGGAEYGDSPAPVIKVPKHIDFEIKIDGRHLKVRDRE
                     TLSLVGPTYDASVENIIMGPGQTAEVALSPQGNALGYSPSAATVSPAISLGEESKKTG
                     YTVTVAAQQAPRGTDLIFVKVPKDKLMWFGDTSTKRRTYKLSIEALTTKSENTYTRSV
                     TIQGHEQAFLYYGPLAKDGTAKIVIYRPGKKKEKVRVIPIK"
     gene            complement(2338..3498)
                     /locus_tag="VFB52_06955"
     CDS             complement(2338..3498)
                     /locus_tag="VFB52_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO06109.1"
                     /translation="METSSLLQLIREGDGSTRADLAKRTGLARSTITQRLEQLQGLGL
                     LKEVAGAESTGGRPPMRLVFNEDAALVLAADLGATHSRLAVTNLGGEVLASDRGDIEI
                     ADGPEVVLEWVEQRFNALLEQAGAGREAVAGIGIGVPGPVEHSTGLPRNPPIMPGWDG
                     FPIPERFADSFGAPVLVDNDVNIMALGEHWSHWRETEHLLFVKVGTGIGCGIIVDGQV
                     HRGADGAAGDLGHVRIPVDEDHADVICSCGNVNCLEALAGGGAMARKLSAEGVEVRDS
                     RALVELTRAGLPAAVQLVRHSGRLIGEVLAAAVNFYNPSVIVIGGDVADAQEQLLASI
                     REVVYQRSLPLATRHLRIVRSPLEDRAGVIGAAAMVTERVLTLDPEFARFSR"
     gene            3585..4757
                     /locus_tag="VFB52_06960"
     CDS             3585..4757
                     /locus_tag="VFB52_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO06110.1"
                     /translation="MEESAVQAGPRSPLASAESLRAGIVGTGFVGRVHARSARLAGAQ
                     IAGVAASTPERSAAARAALGAERDFASAAELIASDAVDVVHVCTPNNLHVPLALAAIE
                     AGKHVICEKPVALDPAEARVLSASAEEAGVLVTVPFVYRYYPTVRELRARLGAAATPA
                     RLLSGGYLQDWLLDAGDYNWRVDPELGGASRAFADIGSHWCDLVEFVSGQRITALVAQ
                     TAIAHETRRGGEAASFERAGGGEERPVSTEDVAALLFETDAGATGSLLVSQVSAGRKN
                     RLWVEITAGAETFAFDQERPDELSVLRRDGATAVPRDFDTLDPSAARYVTLPGGHPQG
                     YQDCFDAFVAESYAAFAGAAPAEGLPVLADGARAVRLTEAVLESAKRGEWIEVKPR"
     gene            4754..5677
                     /locus_tag="VFB52_06965"
     CDS             4754..5677
                     /locus_tag="VFB52_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZO06111.1"
                     /translation="MKLGLLTACLPEWSLERIAVWAGENGYEALELAAWPSLGDRPFT
                     ASHLAADRFDEAEADRVRGTLQMNGLELSALAYYDNNLHPDPAEREAIHAHVRACIDA
                     AAALGGVPVGTFVGRDPGRSVAENLRAAEQVFPPLVDYAGERGVKLMIENCVMEGWTP
                     DGAPGNLAYSPELWEWMFGLGLYLNFDPSHLLWLGIDPVAALRPYVDRVAHAHAKDAE
                     VFPAERNRYGFFGRLAERERDPWDMGWWRYRLPGSGEVDFRRYVDTLYEGGFDGVLSV
                     EHEDPIWGGTPEKVIKGLEIAERNLRPLVVG"
     gene            5710..7215
                     /locus_tag="VFB52_06970"
     CDS             5710..7215
                     /locus_tag="VFB52_06970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO06112.1"
                     /translation="MLEVRGLVKTYPGVEALRGVDFDVRAGEVHCLLGPNGAGKSTLI
                     KCVSGAVEPTAGEILVDGEPLPERDPAAALGRGVATIYQELDLVEDLSVAQNVFLGHE
                     PRRGPLLDLGRMRADAAALLARLGHEKIDPRLPVRALRPAAQQIVSIARALSYDARVL
                     IMDEPSAILDAGEVETLFEVVRRLTAEGVGVVYISHRLDEIRRIGDRVTVLADGATAA
                     GGIAADTPPDELVRLMVGRKVERLYPERPTGGGEVLLEVREARALPAVRGVSFEVRAG
                     EVLGIGGLVGSGRSELLRLICGLDPRETGEVLVAGKRLAPNRPDRAIAAGLGLAPEDR
                     KSQGLLLDWDLTKNITLADLGRFSHGLLDVRGEREAARAQLRALNTVPDDPERRAREL
                     SGGNQQKVVLSRWLLRECRVLLLDEPTRGVDIPSKAEIYRIVTELAGRGLAVVLVSSE
                     LEELAGICTRVLVMREGELVAEVDGAQATERELLAHAVVPTAGPDLIEEVA"
     gene            7212..8204
                     /locus_tag="VFB52_06975"
     CDS             7212..8204
                     /locus_tag="VFB52_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06113.1"
                     /translation="MNQVAALRDRFGALQFQEYALGLVVIALFVTGAILRPETFPTWD
                     NVRNMLTQASIIGVLAVGMTFVIATSGIDLSVGSMVAAAGVFGGILVDPSGGTLLFIA
                     GAIGFAVLLGTINATSITYGRVVPFIATLAMLSIARGLALLLNDKLPVGLLDLNGGSY
                     GDPAPFSLLWFGTGKILGIPVSVWVFLGIVVGGWIALNRTRFGRYVVAVGGNREAARI
                     AGVPVRKIIFSVYVLSGLMAGIATVLLCARLGSASPVSGNLYELDAIGAVVIGGTSLA
                     GGRATMVGTFLGVLTFALIFSLMTQLDLSTEIQQVTKGAIVLGAVLIQRPEAKY"
     gene            8303..9295
                     /locus_tag="VFB52_06980"
     CDS             8303..9295
                     /locus_tag="VFB52_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO06114.1"
                     /translation="MFGVSACGGDTETVTVESESPGGSAEGGGGDSGKTVSIVASVPP
                     TDHGWLGAISKDAQAQADTYDDVDFKLLEAADADSQAQQIEQVISEKPDVLVVLPQDG
                     EALTPVAQKAEAAGIPVVNIDRLFTQPDAATATILGDNYQIGVLAADYIGEELNCKGK
                     VVEIQGLAGISVTDERTKGFEDELKKVCPDGGVEVVAQQPGDFNPDKGLTVMENILQS
                     ENEIDAVYTHDDDMAQGVVQAIRNADRDDEMFLTGVGGSQAAMEQIKEGGLYRATFLY
                     NPNMAASAVNMARLIGLEEGFEELVPPEVPRQVIVPAAVVTKDNVAEYEEYAFD"
BASE COUNT         1377 a         3199 c         3330 g         1492 t
ORIGIN      
        1 cctcggaatt ccggcaggtc gagaagctcg ccgaaggtgg gtgagtcgcc gggtgggcgt
       61 gtcggtgagg tgatgaactt ttcggcggtc gactcgtgca ggacgtacag caccgcgcct
      121 ttggcgtcgc gaatcgggag tcgcccgcgc ggaggggtgg cgtccagcat cttcttcttc
      181 agggctttga ccgacagcgc gcgcgctgcc gcgtcgtcct gcacgtcttc ggcgacgatc
      241 tcggcgagcc cgatcatcac tgcgctgacg tcggtctcgg ccgccgtctc cgatttggcc
      301 agggcgacgg cgctgtcggt ggccgctttc aggttgtcgc gggcgaagta gaaggcgagc
      361 accgtcccga cccaggtccc gaggatcggg atcacgcctg agaacaccat ctgggactcg
      421 tctgcgtctg cggcagaggc gaagatcacg gccgcggcga tcagcaggac gccacccacg
      481 ccggcgagga cgaggatcaa agcgagtgcg aagcggcggt tcgcctcgta ttcggcctgg
      541 gtcttggcta cggtcctcgc catcgccgcg acactcaacc aacctcaatt aaaaagtcaa
      601 acttctactg ttcggttttt aattaggtac ccagcagtgg ttcgcgggac tactacttaa
      661 tcggaatgac ccggactttc tctttctttt tgcccgggcg gtagatcacg atcttcgcgg
      721 tgccgtcttt ggccagcggg ccgtagtaga ggaacgcctg ctcgtggccc tggatcgtca
      781 ccgagcgggt gtaggtgttc tcgctcttcg tggttagggc ctcgatcgag agtttgtagg
      841 tgcggcgttt cgtgctggtg tcgccgaacc acatcagctt gtccttcggc accttgacga
      901 agattaggtc ggtgccgcgc ggggcctgct gggcggcgac ggtgaccgtg taaccggtct
      961 tcttcgattc ctcgccgagc gagatcgcgg gcgagacggt ggcggcgctg gggctgtaac
     1021 cgagcgcgtt gccctgcggc gagagcgcca cttcggcggt ctggcccggg cccatgatga
     1081 tgttttcgac gctggcgtcg taggtcgggc cgaccaggct gagcgtctcg cggtcgcgca
     1141 ctttcaggtg ccgcccgtcg atcttgattt cgaagtcgat gtgcttcggc accttgatca
     1201 ccggcgccgg cgagtcgccg tactcggccc cgccgttggc cagcggcagg atcccggctg
     1261 agctgcgtgg cagcaccttg gcgccgggaa tctggttgac gatccggttg ccgaccgagc
     1321 cggtcttgcg gcccttgtcg tcaacgacga gcaggcgggc gtgctggtcg gcggtgccgt
     1381 cgaggcggat ctcgttgaac ttcgagccgc cgccctggcg gccgacgcag aacgggcagg
     1441 gctggacgcc gagcccggga gtgaccggcg cgagccgcag cgacttcgac ttcgcgttgc
     1501 cttcgtactg ggcctgcggg aagccggggt tgatcgccgc gtagtagctc caggtgttct
     1561 tcttggtgtc gatctgcagg cggcgggtgt cgtcgtcggg ccagttgttg tcgtagacgt
     1621 ggacgtcgta gatgccgttg cccatgttct cgaccgcgta gggggtgatc gcgtggccgg
     1681 ctttgaaccc gtactggaag atctcgaagg tccagatctc cttctcctgc ggattgagtg
     1741 cgccctgcag gagcgtgttg aggatctgca ccggcgtccc gatcagggtg ttttcgttga
     1801 tcgaggagag ggtctggaag ttgaaggcgc gggcgatcga gcgctgcagc agcttgttgc
     1861 ctttgatccc gaggctgaac gtggtggtcg ccccgcggcc gagcccgctg atgctggagt
     1921 agccgaagcg cggcaggtag cccttgtaga tcagctccga gagggttgcg aagccgaagc
     1981 agtgaccgcc gttcgaccct tcgttgaacg ccttcatgta ggtgctggcg atcggcgtca
     2041 acacacactt gcctttgccc ggcatgcaga cgccgggacc gaacagtcgc tcgaactcac
     2101 tcgcgtcgag gttcgcgtag ccctcttcgc caccgtagtt ctggaacgag aacccgttcg
     2161 ggttcgggcg gaagccgttg tcggcgacga tcgtcccggc cgaggcgctc gccggcaggg
     2221 cgagcacgac gacgagcgcc gccagcagca atgccaggcg gggcgagaag cggggggttg
     2281 gacgaagtgg ccgagactcc atcgacccga cttctatcgg cgcgcccggc aattccttta
     2341 acgcgagaag cgggcgaact ccgggtccag ggtgagcacc ctctcggtga ccatcgccgc
     2401 cgcgccgatc acgccggcgc ggtcctcgag cgggctgcgg acgatgcgca ggtggcgggt
     2461 cgcgaggggg agggagcgct ggtagacgac ctcgcggatg ctggcgagga gctgctcctg
     2521 ggcgtcggcg acgtcgccgc cgatcacgat caccgagggg ttgtagaagt tgaccgccgc
     2581 cgccagcacc tcgccgatca ggcggcccga gtggcggacc agctggaccg cggcgggcag
     2641 gccggcgcgg gtcagttcga ccagggcgcg cgagtcccgc acctcgaccc cttcggcgct
     2701 gagcttgcgg gccatcgcgc cgccgccggc cagcgcctcg aggcagttga cgttgccgca
     2761 gctgcagatc acgtccgcgt ggtcctcgtc gaccgggatg cgcacgtggc cgaggtcgcc
     2821 ggcggcgccg tcggccccgc ggtggacctg gccgtcgacg atgatcccgc agccgatccc
     2881 ggtcccgacc ttgacgaaca gcaggtgctc ggtctcgcgc cagtggctcc agtgctcgcc
     2941 cagggccatg atgttgacgt cgttgtcgac caggaccggg gcgccgaagc tgtcggcaaa
     3001 acgctcaggg atcgggaagc cgtcccagcc gggcatgatc ggcgggttgc gcggcagccc
     3061 ggtcgagtgc tcgaccgggc cgggcacgcc gatcccgatc ccggcgaccg cctcacgccc
     3121 ggcgccggcc tgctcgagca gcgcgttgaa gcgctgttcg acccactcga gcacaacttc
     3181 ggggccgtcg gcgatctcga tgtcgccgcg gtcggaggcc agcacctcgc cgccgaggtt
     3241 ggtgaccgcg agccgcgagt gggtggcgcc gaggtcggcg gcgaggacca gcgctgcgtc
     3301 ctcgttgaag accagccgca tcggcggtcg gccgccggtc gactcggcgc cggcgacctc
     3361 cttcagcaac cccagcccct gcagctgctc cagccgctgg gtgatcgtcg agcgagcgag
     3421 cccggtgcgc ttggcgaggt cggcccgggt ggacccgtcg ccttcgcgga tcagctgcag
     3481 cagggagctg gtttccatgg ccgcggaaga gtatggatca cttatgcgtg cctgacaagg
     3541 gacttttgct tgacagtcat gcaaaaggcg gttacgttcg gccaatggag gagagcgccg
     3601 tccaagcggg gcctcgcagc cccttggcaa gcgccgaaag cctgcgcgcc ggcatcgtcg
     3661 gcaccggctt cgtcggccgc gtgcacgccc gctcggcccg ccttgccggc gcccagatcg
     3721 ctggtgtcgc cgcttccact ccggagcgct ccgccgccgc ccgcgccgcg ctcggcgccg
     3781 agcgcgactt cgccagcgcc gccgagctga tcgcctccga cgcggtcgac gtggtccacg
     3841 tctgcacccc gaacaacctg cacgtgccgc tggcgctggc cgcgatcgaa gccggcaagc
     3901 acgtgatctg cgagaagccg gtcgcgctcg acccggccga agcgcgggtg ctgtccgcct
     3961 ccgccgagga ggcgggcgtg ctcgtcaccg tccccttcgt ctaccgctac tacccgaccg
     4021 tgcgcgagct gcgcgcccgg ctcggcgccg ccgccacacc ggcgcggctg ctcagcggcg
     4081 gctacctgca ggactggctg ctcgacgccg gcgactacaa ctggcgggtc gaccccgagc
     4141 tgggcggcgc ctcgcgcgcc ttcgccgaca tcggctcgca ctggtgcgac ctggtcgagt
     4201 tcgtcagcgg ccagcggatc acggcgctgg tcgcgcagac ggcgatcgcc cacgagaccc
     4261 gtcgcggcgg cgaggccgcc tcgttcgagc gtgccggcgg cggcgaggag cggccggtca
     4321 gcaccgagga cgtggcggcg ctgctgttcg agaccgacgc cggggcgacc ggctcgctgc
     4381 tcgtctcgca ggtctcggcc gggcgcaaga accggctctg ggtcgaaatc acggccggtg
     4441 ccgaaacctt cgccttcgac caagaacgac cggacgagct gtcggtgctg cggcgcgacg
     4501 gcgcgaccgc ggtcccgcgc gacttcgaca cgctcgaccc gagcgcggcc cgctacgtga
     4561 ccctgcccgg gggccacccg cagggctacc aggactgctt cgacgccttc gtcgccgaga
     4621 gctatgccgc cttcgccggc gccgctccgg ccgagggcct gcccgtgctc gcggatggcg
     4681 cccgcgccgt tcgcctcacc gaggcggtgc tggagtcggc aaagcgcggc gagtggatcg
     4741 aggtgaagcc gcgatgaagc tcggcctgct gaccgcctgc ctgcccgagt ggtcgctcga
     4801 gcggatcgcc gtgtgggcgg gggagaacgg ctacgaagcg ctcgagctgg ccgcctggcc
     4861 ctcgctcggc gaccggccct tcaccgcctc ccatctcgcc gccgaccggt tcgacgaggc
     4921 cgaggccgac cgtgtccgcg gcacattgca gatgaacggg ctggagctct cggcgctcgc
     4981 ctactacgac aacaacctgc accccgaccc ggccgagcgc gaggcgatcc acgcccacgt
     5041 gcgtgcttgt atcgacgccg ccgcggcgct cggcggcgtc ccggtcggca ccttcgtcgg
     5101 tcgcgatcct ggccgcagcg tcgccgagaa cctgcgcgcg gccgagcagg tcttcccgcc
     5161 gctggtcgac tacgccggcg agcgcggggt caagctgatg atcgagaact gcgtgatgga
     5221 gggctggacg cccgacggcg cccccggcaa cctcgcctac tcgccggagc tctgggagtg
     5281 gatgttcggg ctcgggctct acctcaactt cgacccctcg cacctgctct ggctcgggat
     5341 tgacccggtg gcggcgctgc ggccgtatgt ggaccgggtc gcccacgcgc acgccaagga
     5401 cgccgaggtc ttcccggccg agcgcaaccg ctacggcttc ttcggccggc tcgccgagcg
     5461 tgagcgcgac ccttgggaca tgggctggtg gcgctaccgg ctgccgggct cgggcgaagt
     5521 cgacttccgc cgctacgtcg acacgctgta cgagggcgga ttcgacgggg tgctctcggt
     5581 cgagcacgag gacccgatct ggggcggcac tccggagaaa gtgatcaagg gcttggagat
     5641 cgccgagcgc aatctgcggc cgctggtggt cggatgaacg gagcggctga caagcaaggt
     5701 gccccgacgg tgctcgaggt ccgcggcctg gtcaagacct accccggggt cgaggcgctg
     5761 cgcggggtcg acttcgacgt gcgcgccggc gaggtccact gcctgctcgg tcccaacggt
     5821 gccggcaagt cgacgctgat caagtgcgtc tcgggcgcgg tcgaaccgac cgccggcgag
     5881 atcctggtcg acggcgagcc gctgcccgaa cgcgacccgg cggcggcgct cggccgcggc
     5941 gtggcgacga tctaccagga gctcgacctg gttgaggacc tcagcgtcgc ccagaacgtc
     6001 ttcctcggcc acgagccgcg gcgcggcccg ctgctcgacc tcggccggat gcgcgccgac
     6061 gccgccgcgc tgctcgcccg gctcggccac gagaagatcg acccgcggct gccggtgcgg
     6121 gcgctgcgcc cggccgccca gcagatcgtc tcgatcgccc gcgcgctctc ctacgacgcc
     6181 cgcgtgctga tcatggacga gccctcggcg atcctcgacg cgggcgaggt cgagaccctg
     6241 ttcgaggtgg tgcgccggct caccgccgaa ggcgtcggcg tcgtctacat ctcccaccgc
     6301 ctcgacgaga tccgccggat cggcgaccgg gtcaccgtgc tcgccgacgg cgccaccgcc
     6361 gctggcggga tcgccgccga caccccgccc gacgagctgg tccggctgat ggtcggccgc
     6421 aaggtcgagc gcctctaccc ggagcgcccg accggcggcg gcgaggtcct gctcgaggtt
     6481 cgcgaggcgc gcgccctgcc tgccgtgcgc ggggtcagct tcgaggtgcg ggcgggggag
     6541 gtgctcggga tcggcggcct ggtcggctcc gggcgcagcg agctgctgcg gctgatctgc
     6601 gggctcgatc cgcgcgaaac cggcgaggtg ctggtcgccg ggaagcgcct ggcgccgaac
     6661 cggcccgacc gcgcgatcgc cgccgggctc gggctcgccc ccgaggaccg caagtcgcag
     6721 ggcctcctgc tcgactggga cctgaccaag aacatcacgc tcgccgacct cggccgtttc
     6781 agccacggcc tgctcgacgt ccgcggcgag cgcgaggcgg cgcgggcgca gctgcgcgcc
     6841 ctcaacaccg tgcccgacga ccccgagcgc cgcgcccgcg agctctccgg cggcaaccag
     6901 cagaaggtcg tgctctcccg ctggcttctg cgcgaatgcc gcgtgctcct gctcgacgag
     6961 ccgacccgcg gggtcgacat cccgagcaag gccgagatct accggatcgt caccgagctg
     7021 gcagggcgcg ggctcgcggt cgtgctcgtc tcctccgagc tggaggagct ggcggggatc
     7081 tgcacgcgcg tgctggtgat gcgcgagggc gagttggtcg ccgaggtcga cggtgcccag
     7141 gcgaccgagc gcgagctgct cgcacacgcg gtcgtcccga ccgccggacc ggacctgatc
     7201 gaggaggtcg catgaaccaa gtcgccgcac tgcgagaccg cttcggggcg ctgcagttcc
     7261 aggagtacgc gctcgggctg gtcgtgatcg cgctgtttgt gaccggcgcg atcctccggc
     7321 cggagacttt cccgacctgg gacaacgtcc gcaacatgct cacccaggcg agcatcatcg
     7381 gggtgctcgc ggtcgggatg accttcgtga tcgcgacctc cgggatcgac ctctcggtcg
     7441 gctcgatggt cgcggcggcc ggggtcttcg gcggcatcct cgtcgacccg agcggcggca
     7501 ccctgctctt catcgccggc gcgatcggct tcgcggtgct gctcgggacg atcaacgcga
     7561 cctcgatcac ctacggccgc gtcgtcccct tcatcgccac cctggcgatg ctctcgatcg
     7621 cccgaggctt ggcgctgctg ttgaacgaca agctcccggt cggcctgctc gacctcaacg
     7681 gcggctccta cggcgacccg gccccgttct cgctgctctg gttcgggacc gggaaaatcc
     7741 tcgggatccc ggtctcggtc tgggtcttcc tcgggatcgt cgtcggcggc tggatcgcgc
     7801 tcaaccgcac ccgcttcggc cgctacgtgg tcgccgtcgg cggcaaccgc gaggcggcgc
     7861 ggatcgccgg cgtgccggtt cgcaaaatca tcttcagcgt ctacgtgctc tccggcctga
     7921 tggccgggat cgcgaccgtg ctgctctgcg cgcgcctcgg cagcgcctcg ccggtcagcg
     7981 gcaacctcta cgagctcgac gcgatcggcg ccgtcgtgat cggcggcacc agcctggccg
     8041 gcggccgggc gacgatggtc gggacgttcc tcggcgtcct caccttcgcc ctgatcttct
     8101 ccctgatgac ccagctcgat ctctcgactg agatccagca ggtgaccaaa ggggcaatcg
     8161 tcctcggcgc ggttctgatc cagcgacccg aggcgaagta ctgaggggct atggacttct
     8221 catgcgggaa atgtcaacaa aggaggaggg aatcatgtcg ctgaagcgac tgatgctcgc
     8281 gattggggtc gcgggcctac tggtgtttgg ggtatcagcc tgcggcggcg acaccgagac
     8341 ggtgaccgtc gaaagcgaga gcccgggggg gtcggccgaa ggtggtggcg gcgactccgg
     8401 caaaacggtg agcatcgtcg ccagcgtgcc gccaaccgac cacggctggc tgggggcgat
     8461 ctccaaggac gcccaggcgc aggccgacac ctacgacgac gtcgatttca aactgctcga
     8521 ggcggccgac gcggattcgc aggcgcagca gatcgagcag gtgatctcgg aaaaaccgga
     8581 cgtgttggtg gtgctgccgc aggacggcga agcgctgacg ccggtcgctc agaaagccga
     8641 ggcggcgggg atcccggtgg tcaacatcga ccgcctgttc acccagccgg acgcggcgac
     8701 ggcgacgatt ctcggcgaca actaccaaat cggggttctg gcggccgact acatcggcga
     8761 agaactcaac tgcaaaggca aggtcgtcga aatccagggt ctggccggga tctcggtcac
     8821 cgacgaacgg accaaagggt tcgaagacga gttgaaaaag gtctgcccgg acggcggggt
     8881 cgaagtcgtc gcccagcagc cgggtgactt caacccggac aaaggcctga ccgtgatgga
     8941 gaacatcctc cagtcggaga acgaaatcga cgcggtctac acccatgacg acgacatggc
     9001 ccagggcgtc gtgcaggcga tccgcaatgc cgaccgggac gacgaaatgt tcctcaccgg
     9061 cgtcggcggc tcgcaggcgg cgatggaaca gatcaaagaa ggcggcctct accgggcgac
     9121 cttcctctac aacccgaaca tggccgcctc ggccgtgaac atggcccgcc tgatcgggct
     9181 cgaagaaggg ttcgaggagc tggtcccgcc ggaggttccg cgccaggtga tcgtgcccgc
     9241 cgcggtggtg accaaagaca acgtcgccga atacgaagaa tacgcgttcg actgagactc
     9301 tcggccaagg agggggcgcg ccgcggggcc accgcggcgc gcccgttcaa cccatcgggg
     9361 cgaggctcat cgacaacttg gccgcttgac cgatgccg
//
LOCUS       DASRIG010000255         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1436055, whole genome shotgun sequence.
ACCESSION   DASRIG010000255 DASRIG010000000
VERSION     DASRIG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436055"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFB52_06985"
     CDS             complement(<1..673)
                     /locus_tag="VFB52_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06115.1"
                     /translation="MSRRAGSARTLEIFAVLLAAGTCFATAQTLVVPALPEITEKFDT
                     SAVAASWLVTGFLLSAAIATPIAGKLGDVYGKGRVLIWVLLIFAAGSVICALAEGIGP
                     MIGGRVLQGVSGGVYPLAYATVKETFPPEKVPNGLSMVSVLVGFGGAVGLLLAGPIID
                     NLGISWLFWFGLIALPTAVATWVLIPSTRAAAGTRVDWRGAALLSASLACVLLAITRA
                     NDWGWG"
     gene            complement(670..1833)
                     /locus_tag="VFB52_06990"
     CDS             complement(670..1833)
                     /locus_tag="VFB52_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06116.1"
                     /translation="MSVDVGEGLYSIPQEMVDFRETIRQIAVEKIAPRAGEIDRTAEY
                     PWDIRKLLAEQDILGLPFDEEYGGTGTGTLMLQIGVEEIAKACASCALILMINELGTL
                     PIQLFGSDEQKERFLPKCASGEWSPAFALSEPEAGSDPAAMRTTAVKDGDEWVINGQK
                     AWITNAGVADFYVVFCSTDRENRRTSAFIVEKDREGFDPGQLEHKLGIKGSPTGSPSF
                     TDVRVPHENLVGEEGKGLHVALGTLERTRLGAAAQAVGIAQGATDFANHYASERIAFG
                     KPINQLQAIQFKLADMEAGTAAARELLYKACALADMPQRPALLPKYTSMAKLIASDNA
                     MKVTVEAIQVLGGYGYVNEYPVERMMRDAKITQIYEGTNEIQRVVIARSMSPQ"
     gene            complement(1830..2705)
                     /locus_tag="VFB52_06995"
     CDS             complement(1830..2705)
                     /locus_tag="VFB52_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZO06117.1"
                     /translation="MAIERVAVLGGGLMGSGIAESVARAGLPVVVRDVDETACGAVRE
                     RVEASLYQATSRGKVDAAGAEAIRERIILTTELAEIAEADLVIEAVPENAELKAGILT
                     QVAGIVSADALIASNTSSIPIAQLAANVHRPERVLGLHFFSPVPVMPLVEIVVALDTS
                     EETVDAASEFADLIAKKPIVTKDRSGFIVNMLLVPYLMAAVRMYEEGFADREAIDEGM
                     KLGCGHPMGPLTLSDFIGLDVLYAIGDSLYEEFKRAEYAPPPLLKRMVASGRIGRKAK
                     RGFYDYGPVREGATG"
     gene            complement(3314..3820)
                     /locus_tag="VFB52_07000"
     CDS             complement(3314..3820)
                     /locus_tag="VFB52_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO06118.1"
                     /translation="MSSPRLPFDPIEEARRHWLARWPEQAATEMVAVTSIMRVHQILM
                     ARLNELLEPFDLTFPRYEALMLLHFSSRGSLPLGKIGERLQVHRTSVTNTIDGLERSG
                     LVRRVPHESDRRAVLAEILPAGREVAESATAALNGADFGTLPLDDEDLEALFGTLRKL
                     RDGAGDFD"
     gene            complement(3871..4536)
                     /locus_tag="VFB52_07005"
     CDS             complement(3871..4536)
                     /locus_tag="VFB52_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06119.1"
                     /translation="MGDSLEESTGPNIPKYLPGVEVTVNAVGGSNTYEIRELFEESWD
                     PSYSVVVFDGGTNDNPNYPEILAENLAKVAATVGNRCMVVPTIHGYTVEGVNNRGKNR
                     VVHEFAASRPGTQTPDWATVANTQPEMMQPDNLHPSEEGADYRAQLIADGIAGCLAYV
                     ETSALPAPVEETAAASTVETEEFEPVAKMARREQQLLREMGGEMLRQVATVTAFKVLF
                     PGG"
     gene            4665..>4956
                     /locus_tag="VFB52_07010"
     CDS             4665..>4956
                     /locus_tag="VFB52_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06120.1"
                     /translation="MILASRAASRATLAACALALAALVLALSGCGGDGSASAGDATVE
                     VTAKPTQTTKLATKRSPVGDRCRKQVGGFLDSMGALRQLLVDGLSYEQYVEEI"
BASE COUNT          745 a         1718 c         1703 g          790 t
ORIGIN      
        1 acccccagcc ccaatcgttg gcgcgggtga tcgccaacag cacgcaggcc agcgaagccg
       61 agagcagcgc cgcgccgcgc cagtcgaccc gcgtccccgc ggcggcccgg gtcgagggga
      121 tcaggaccca cgtcgccacc gcggtcggca gcgcgatcag gccgaaccag aagagccagg
      181 agatccccag gttgtcgatg atcggcccgg cgagcagcag cccgacggcg ccgccgaacc
      241 cgaccaggac gctgaccatc gagagcccgt tggggacctt ctcgggcggg aaggtctctt
      301 tcaccgtcgc gtaggcgagc gggtagacgc cgcccgagac cccctgcagg acgcgcccgc
      361 cgatcatcgg cccgatcccc tcggcgagcg cgcagatcac gctgccggcg gcgaagatca
      421 agagcaccca gatcagcacc cggcccttgc cgtagacgtc gccgagcttg ccggcgatcg
      481 gggtcgcgat cgccgccgag agcaggaacc cggtgaccag ccaggacgcc gcgaccgccg
      541 aggtgtcgaa cttctcggtg atctccggca gcgccgggac gaccagcgtc tgcgcggtgg
      601 cgaagcaggt gcccgccgcc agcaggacag cgaagatctc gagcgtgcgc gccgatccgg
      661 cgcgcctgct cactgcggcg acatcgagcg ggcgatcacg acccgctgga tctcgttcgt
      721 gccctcgtag atctgggtga tcttggcgtc gcgcatcatc cgctcgaccg gatactcgtt
      781 gacgtagccg tagccgccga gcacctggat cgcctcgacc gtgactttca tcgcgttgtc
      841 ggaggcgatc agcttcgcca tcgaggtgta cttcggaagc agggcgggac gctgcggcat
      901 gtcggccagc gcgcaggctt tgtagagcag ctcgcgcgcg gcggcggtgc cggcctccat
      961 gtcggcgagc ttgaactgga tcgcctgcag ctggttgatc ggcttgccga aggcgatccg
     1021 ctcggacgcg tagtggttgg cgaagtcggt cgcgccctgg gcgatcccga ccgcctgcgc
     1081 ggcggcgccg aggcgggtgc gctcgagcgt gccgagcgcg acgtgcaggc ccttgccttc
     1141 ctcgccgacc aggttctcgt gcggcacgcg cacgtcggtg aagctcggcg agccggtcgg
     1201 cgagcccttg atcccgagct tgtgctcgag ctggccgggg tcgaagccct cgcggtcttt
     1261 ctcgacgatg aaggcgctgg tgcggcggtt ctcgcgatcg gtcgaacaga agacgacgta
     1321 gaaatcagcg acgccagcgt tggtgatcca ggccttctgc ccgttgatca cccattcgtc
     1381 gccgtctttg actgcggtgg tccgcatcgc cgccggatcc gaacccgcct ccggctccga
     1441 gagcgcgaag gccggcgacc actcgccgga cgcgcacttc ggcaggaagc gttctttctg
     1501 ctcgtcgctg ccgaacagct ggatcggcag cgtcccaagc tcgttgatca tcaggatcag
     1561 ggcacaggag gcgcaggcct tggcgatctc ctcgaccccg atctgcagca tcagcgtgcc
     1621 ggtcccggtg ccgccgtact cctcgtcgaa gggcagcccg aggatgtcct gctcggcgag
     1681 cagcttgcgg atgtcccacg ggtactcggc ggtgcggtcg atctcgccgg cccgcggagc
     1741 gatcttctcg accgcgatct ggcggatcgt ttcgcggaag tcgaccatct cctgggggat
     1801 cgagtagagc ccctctccaa cgtcgacgct catcccgttg ccccctctcg taccggtccg
     1861 tagtcgtaga aaccgcgctt tgccttgcgc ccgatccgcc ccgaggcgac catccgcttc
     1921 agcagcggcg gcggcgcgta ctccgcgcgc ttgaactcct cgtagagcga gtccccgatc
     1981 gcgtagagca cgtcgaggcc gatgaagtcg ctcagcgtca gcggtcccat cgggtgcccg
     2041 cagcctagct tcatcccctc gtcgatcgcc tcgcggtcgg cgaacccttc ttcgtacatc
     2101 cgcaccgccg ccatcaggta gggcacgagc agcatgttga cgatgaagcc cgagcggtcc
     2161 ttggtcacga tcggcttctt cgcgatcaga tcggcgaact cgctggccgc gtcgacggtc
     2221 tcctcggagg tgtcgagggc gacgacgatc tcgaccagcg gcatcaccgg caccgggctg
     2281 aagaagtgca gcccgagcac gcgctcgggg cggtggacgt tggcggcgag ctgggcgatc
     2341 gggatcgagg aagtgttgga ggcgatcagc gcgtctgcgg agacgatccc ggcgacctgg
     2401 gtgaggatgc ccgccttcag ctcggcgttc tccggcaccg cctcgatcac caggtcggcc
     2461 tcggcgatct cggccagctc ggtggtgagg atgatccgtt cgcggatcgc ctcggcgccg
     2521 gcggcgtcga ccttgccgcg cgaggtcgcc tggtagagcg aagcctcgac ccgctcgcgg
     2581 accgccccgc aggcggtctc gtcgacgtcg cggacgacaa ccgggagccc ggcgcgcgcg
     2641 accgactcgg cgatgcccga gcccatcaga ccgccgccga gcaccgcgac gcgctcgatc
     2701 gccatcagcg gcccgctgtc tccgtgcaga tcgagagtcg ccccctgtac agcgaggcgc
     2761 ggggccctct ggtgaaggga tgggggccca agcggccggt gcagctgggg gaagcagcgg
     2821 ccgtcgttgc ctcgatgcac aggggatgta tccgacggcc ggaagccttg gagagcttca
     2881 ccgcgccagg ggttgcgcgg ttgaggacgt cagatacgcg actcacagat agtaggaata
     2941 ccacgtatct cagaccgccg caacgccgcc cccctcccca ttccgccagt cggggggtgg
     3001 gcagtggcca tcgacgccgg gctccccatc gcgcggttcc tttcccgaca aatggacccg
     3061 aaaggaacca cgggattcgt cagcttccgc gaccccgtcc ctgaccccgc tatccggggc
     3121 taggaacggg ctcgcgaaag ccagacggaa tccccgggat ggcccgtgtg gaggggtcgg
     3181 gtgactcccg gaacgccttg tacttttttg cttcagcgaa tcgaggcgta aagccgccct
     3241 cgatccacaa aaaagttaag tggagggagt cacccgaccc ctccccgcat gcgatcccgg
     3301 agtacgcccg tcctcagtcg aaatcgcccg cgccgtcgcg cagcttgcgc aacgtgccga
     3361 acagcgcttc gaggtcctcg tcgtcgagcg gcagcgtgcc gaagtcggcc ccgttgaggg
     3421 cggcggtggc ggactcggcg acctcgcggc cggccgggag gatctcggcg agcacggccc
     3481 ggcggtcgct ctcgtggggg acgcggcgga ccaggccgga gcgctcgagg ccgtcgatcg
     3541 tgttggtgac gctggtccgg tggacctgca ggcgctcgcc gatcttgccc agcggcagcg
     3601 agccgcggct gctgaagtgg agcagcatca gcgcctcgta gcggggaaag gtgaggtcga
     3661 acggctccag cagctcgttc agccgcgcca tcaggatctg gtgcacgcgc atgatcgagg
     3721 tgaccgcgac catctccgtc gccgcctgtt cgggccagcg ggccagccag tggcggcggg
     3781 cctcctcgat cgggtcgaag gggagtcgcg gggacgacat ggccgggcag tgtgacagcg
     3841 ccgcccgtca gcggggcgcc gcgggggcga tcagccgccc gggaacagca ccttgaacgc
     3901 ggtcaccgtg gccacctgac gcagcatctc gccgcccatc tcacgcagca gctgctgttc
     3961 gcgccgggcc atcttcgcga ccggctcgaa ctcctcggtc tcgaccgtcg aagcggccgc
     4021 ggtttcttcg accggcgccg gcagggcgct ggtttcgacg taggcgaggc agccggcgat
     4081 cccgtcggcg atcagctggg cgcggtagtc ggccccctct tcgctcgggt gcaggttgtc
     4141 gggctgcatc atctccggct gcgtgttcgc aaccgtcgcc cagtcgggcg tctgcgtgcc
     4201 cgggcgcgag gcggcgaatt cgtgcacgac ccggttcttg ccgcggttgt tgacgccttc
     4261 gaccgtgtag ccgtggatcg tcggcacgac catgcagcgg ttgccgaccg tggccgccac
     4321 cttggccagg ttttcggcga ggatctcggg atagttcggg ttgtcgttgg tgccgccgtc
     4381 gaagacgacc accgagtacg acgggtccca gctttcttcg aagagttcgc ggatttcgta
     4441 ggtgttggag ccgccgaccg cgttgaccgt gacttccacc ccgggcaggt acttggggat
     4501 gttcgggccg gtcgattcct ccaggctgtc gcccacgacc agcacttttg gcgcagcgct
     4561 cgcggccgcc gggaccgtca gtcccagcgc caccaatgcg atcgcgaccc ctgccgcggc
     4621 gtgcctgcgc gctttccgca tcagccaact tctacattac gcgcatgatc ctggcttccc
     4681 gagcggcatc gcgggcgacc ttggccgcct gcgcgctggc cctcgcggcg ctcgttctcg
     4741 ccctctccgg ctgcggcggc gacggctccg cgagcgcggg cgacgcgacc gtcgaggtaa
     4801 ccgcgaagcc gacgcagacg acgaagctgg cgaccaagcg ctcgccggtc ggcgaccgct
     4861 gccgcaagca ggtcggcggc ttcctcgact cgatgggcgc cctccgccag ctgctcgtcg
     4921 acggcctcag ctacgaacag tacgtcgaag aaatcg
//
LOCUS       DASRIG010000256         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_377886, whole genome shotgun sequence.
ACCESSION   DASRIG010000256 DASRIG010000000
VERSION     DASRIG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377886"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFB52_07015"
     CDS             <1..658
                     /locus_tag="VFB52_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06121.1"
                     /translation="LARDGAKVTGLDVPQAGEDLGRLMTELSGDSLTLDITAADAPER
                     IAERFADGLDVLVHNAGVTKDRTIAKMPEERWTQLMEINLSSEERINDALIDGGMIGS
                     GGRIVCVSSMSGIAGNSGQTNYAASKAGVIGMVEALAPELAARGATINAVAPGFIETK
                     MTAAMPIGPREAGRRLSSLSQGGLPVDVAETIAWFASPASTGVTGNVVRVCGQSLLGA
                     "
     gene            660..1592
                     /locus_tag="VFB52_07020"
     CDS             660..1592
                     /locus_tag="VFB52_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO06122.1"
                     /translation="MGTLSLDSAPSIPLLYARAAAPLIPGASLLPFIPGGGGEIPELD
                     LELAGVKADPADVAAYAKVCGFTLRDALPPTYPHILAFPLQMAVMADGSFPFGAVGLV
                     HLENRIVQRRPLAISEELTLRVRPTKLLPHPKGRTFTLVTEASADGEVAWESVSTFLR
                     RGKGEEATATGERFATVPEETPASAEWRLDGGLGRRYAAVSGDRNPIHMHSLTAKPLG
                     FPAAIAHGMWTKARCLAALESRLPDAFAVDVRFRKPILLPTKVEFASAEISDGMQHDE
                     IAFTVRDAKRGSPHLDGLIRPNHPNTNAKSGRKK"
     gene            1589..>2838
                     /locus_tag="VFB52_07025"
     CDS             1589..>2838
                     /locus_tag="VFB52_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06123.1"
                     /translation="MSTTTTDTDQGMAERSMAFGLRALNKLAGSDLLDRIRIRKGVEK
                     ALFQGTKNGFRSATVAGRTFKAAQQLGKPARQKKSKSKGLFDVTPDDEQQMFQEAGRA
                     FAEEKIRPAALAADDACATPAELLEATAELGVNMLGIPEELDGVMHEQSAVTSVLIGE
                     ALAHGDMGIAYAALAPGAVATAIGLWGSAEQEATYLPAFTGENVPAAALAILEPRPLF
                     DPLVLQTTARKDGDEWVLDGAKSLLARAADCELFVIAAEAQGIGPTLFVAESGTKGLA
                     VEPEPAMGLRPAATGRLVIEGARLPAGALLGEGRPEDYVECINRARIAWCGLAVGTAQ
                     AVLDYVIPYVNDRQAFGEPISNRQGVAFAVSDVGIETGGMRLATYRAASRADRGEEFA
                     REAAIARKLCSAKGMQIGSDGVQLL"
BASE COUNT          439 a         1086 c          958 g          355 t
ORIGIN      
        1 gctcgcccgc gacggcgcca aggtcaccgg cctcgacgtg ccccaggccg gcgaagacct
       61 cggccgcctg atgaccgagc tgagcggcga ctcgctgacc ctcgacatca ccgccgccga
      121 cgctcccgag cgaatcgccg agcgtttcgc cgacgggctc gacgtgctcg tccacaacgc
      181 cggagtgacc aaagaccgga cgatcgcgaa gatgccggag gagcgctgga cgcagctgat
      241 ggagatcaac ctctccagcg aggagcggat caacgacgcc ctgatcgacg gcggcatgat
      301 cggctccggc ggccggatcg tctgcgtctc ctcgatgtcg gggatcgccg gcaactcggg
      361 ccagaccaat tacgccgcct cgaaggcggg cgtgatcggg atggtcgagg cgctggcccc
      421 cgaactcgcc gcgcgcggcg ccacgatcaa cgcggtcgcc cccggcttca tcgagacgaa
      481 gatgaccgcg gcgatgccga tcggcccgcg cgaagccggc cgccgcctct cctcgctctc
      541 acagggcggc ctgcccgtcg acgtcgccga gacgatcgcc tggttcgcca gccccgcctc
      601 taccggggtg accggaaacg tcgtccgcgt ctgcggacag agcttgctgg gggcctgaga
      661 tgggaactct ctccctcgac agcgccccca gcattcccct cctctacgcc cgcgccgcgg
      721 cgccgctgat ccccggtgcc tcgctgctgc ccttcatccc gggcggcggt ggcgagatcc
      781 cggagctgga cctcgagctg gccggagtca aggccgaccc cgccgacgtc gccgcctacg
      841 ccaaggtctg cggcttcacc ctgcgcgacg cgctgccgcc gacctacccc cacatcctcg
      901 ccttcccgct gcagatggcg gtgatggccg acggcagctt ccccttcggc gccgtcggcc
      961 tcgtccacct cgagaaccgg atcgtccagc gccgcccgct cgcgatcagc gaggagctga
     1021 ccctgcgcgt gcgcccgacc aagctgctcc cccaccccaa gggccgcacc ttcaccctgg
     1081 tcaccgaggc gagcgccgac ggcgaggtcg cttgggaaag cgtcagcacc ttcctgcgcc
     1141 gcggcaaggg cgaggaggcg accgcgaccg gcgagcgctt cgcgaccgtg ccggaggaga
     1201 ccccggcgag cgccgagtgg cgcctcgacg gcggcctcgg gcgccgctac gccgccgtct
     1261 ccggcgaccg caacccgatc cacatgcact cgctgacggc gaagccactc ggcttcccgg
     1321 ccgcgatcgc gcacgggatg tggaccaagg cgcgctgcct ggcggcgctg gagagccgcc
     1381 tccccgacgc cttcgccgtc gacgtccgct tccgcaagcc gatcctgctg ccgaccaagg
     1441 tcgagttcgc cagcgcggaa atcagcgacg gcatgcaaca cgacgagatc gccttcaccg
     1501 tccgcgacgc caagcgcggc agcccccacc tcgacgggct gatccgcccc aaccacccca
     1561 acacgaacgc caaatccggg aggaagaagt gagcaccacg accaccgaca ccgatcaggg
     1621 gatggccgag cgcagcatgg ccttcggcct gcgggcgctg aacaagctcg ccggctcgga
     1681 cctgctcgac cgcatccgca tccgcaaggg ggtcgagaag gcgctcttcc agggcaccaa
     1741 gaacggcttc cgctcagcca ccgttgccgg gcgcaccttc aaggccgcgc agcagctggg
     1801 caaaccggcg cgccagaaga agtcgaaatc caagggcctg ttcgacgtca ctcccgacga
     1861 cgagcagcag atgttccagg aagccggccg cgccttcgcc gaggagaaga tccgaccggc
     1921 ggcgctcgcc gccgacgacg cctgcgcgac cccggctgag ctgctcgagg cgaccgcgga
     1981 gctcggcgtc aacatgctcg gcatccccga ggagctcgac ggcgtgatgc acgagcagtc
     2041 ggcggtgacc agcgtgctga tcggcgaggc gctggcccac ggcgacatgg ggatcgccta
     2101 cgcggcgctc gcccccggcg ccgtcgccac cgcgatcggg ctctggggca gcgccgagca
     2161 ggaggccacc tacctgccgg cgttcaccgg cgagaacgta cccgcggcgg cgctcgcgat
     2221 cctcgagccg cgtcccctgt tcgacccgct ggtgctgcag acgaccgccc gcaaagacgg
     2281 cgacgagtgg gtcctcgacg gggccaagtc gctgctcgcc cgggctgcgg actgcgagct
     2341 gttcgtgatc gcggccgagg cccagggcat cggcccgacc ctgttcgtcg ccgagtccgg
     2401 gaccaagggg ctcgcggtcg agccggagcc ggcgatgggg ctgcgtcccg ccgccaccgg
     2461 ccggctggtg atcgagggtg cccggctgcc ggccggcgcg ctgctcggcg agggccgccc
     2521 cgaggactac gtcgagtgca tcaaccgggc gcggatcgcc tggtgcgggc tcgcggtcgg
     2581 caccgcgcag gcggtgctcg actacgtgat cccctacgtc aacgaccgcc aggccttcgg
     2641 cgaaccgatc tccaaccgcc agggcgtcgc cttcgcggtc tccgacgtcg gcatcgagac
     2701 cggcggcatg cggctcgcca cctaccgggc ggcctcgcgc gccgaccgcg gcgaggagtt
     2761 cgcccgcgag gcggcgatcg cacgcaagct ctgcagcgcc aaggggatgc agatcggctc
     2821 cgacggcgtg cagctgct
//
LOCUS       DASRIG010000257        10732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3515604, whole genome shotgun sequence.
ACCESSION   DASRIG010000257 DASRIG010000000
VERSION     DASRIG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515604"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1520)
                     /locus_tag="VFB52_07030"
     CDS             complement(84..1520)
                     /locus_tag="VFB52_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO06124.1"
                     /translation="MATIDTAAPLERPKEMSANPVRRVAEDVAAEWRGRGQFPPGPTD
                     FSFARTHQAAHDPLPLLLGAYEDFGPIFSMRLLHTRVIFMLGPEANHFVTVAHPENFH
                     WRESSFGDLIPLLGDGLLTIDDAFHDRARAIMMPAFHREQVAIATAAMTAEAEVAIAG
                     LQVGGVVDVYDWMRNLAMRIAMRALLGLDPDEAGKGAAAAEHFERALGFYGIDFALRL
                     LRGPGSPWRKMNASREVLDEIVFGEIARRRAKPDPERRDILSLLVNVRGEDGDSFSDR
                     EVRDQVMTLMFAGHDTSTSTVTFMLHELARHPDVLEKLCAEQDEVLGGRPPTIDQLER
                     EMPYLDMVLDEVLRLYPPAWIGPRRAVRDFEFGGYAVPRGAYANYCSWASHRIPSVFP
                     DPEAFIPERFTRERKAALPRGAYVPFGGGQRVCIGKRFGQIEVKLVASMLLQQLRLDA
                     LPGRTMTIRQMPTLSPKGGLRMRVLPRA"
     gene            1656..2429
                     /locus_tag="VFB52_07035"
     CDS             1656..2429
                     /locus_tag="VFB52_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06125.1"
                     /translation="MHGKLRLSLLAALCLLGVSVIVASATAETATVGNIKFTADGGFS
                     PKALPKKTFAPIALTAKGKIETKDGTHPPALKEVLLETDKNGAIDVKGYPACTSGKLQ
                     SQTTANAEKACKGTLIGTGKTTVEIELADQPPIDAKSKLLVVNGGVKGGVTTLFIHAY
                     LTQPVPAAVVTTVKIKKINKGRYGTLSVASIPVIASGAGSVTDFELTVDKKFTYKGKQ
                     KSVLTAKCPDGKLQANATAIFRDGTRVSQDFVRPCTGKG"
     gene            complement(2478..3461)
                     /gene="rlmN"
                     /locus_tag="VFB52_07040"
     CDS             complement(2478..3461)
                     /gene="rlmN"
                     /locus_tag="VFB52_07040"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZO06126.1"
                     /translation="MDRDLLESTLAETGEPGYRASQIWEWVARGARSYAEMTNLPAAL
                     RERLEAAVPLSTLAVEAEAHSDDGTVKTLFQTADGRPIEAVLMRYRDGRRSVCVSSQS
                     GCPLTCTFCATGQMKFGRNLGADEILDQALHFRRAEAIDHVVFMGMGEPTMNIDNVLA
                     ACERLPDLGVTHRRTAISTVGWVPGIDRLAECEMPIALALSLHAPNDGLRSQLMPVND
                     RYPLADVLAACDRYRGARRRKVFVEYVMLDGVNDLPEHARELAALLDPRVYKVNLIPY
                     NPTGAYDGSSPERIERFRSILAEQRLPATVRLTRGRDIDAACGQLAAKAAA"
     gene            complement(3513..4088)
                     /locus_tag="VFB52_07045"
     CDS             complement(3513..4088)
                     /locus_tag="VFB52_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZO06127.1"
                     /translation="MADQLPTLLFASADEFEAWLEDHHAEPGGIWLQIAKKGAPEPSV
                     TYAEAVELALCFGWIDGQRRALDATHFLQRFTPRRPRGRWSKINREKAEALIADERVR
                     PAGMAEIEAARADGRWEAAYEGQRTAKVPDDLQRELDANPAAAAFFAELDSANRYAIV
                     YRLGEAKKPETRERRLRKFVAMLERGEKIHG"
     gene            complement(4081..4359)
                     /locus_tag="VFB52_07050"
     CDS             complement(4081..4359)
                     /locus_tag="VFB52_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZO06128.1"
                     /translation="MAGARIAVRLRPRGHADELLGLEDGVLQARVAAPPVDGKANKAL
                     CRLIAKRVGVAPSRVGVARGAKSREKLIEVEGVGQDELLAALSGGDDG"
     gene            complement(4370..5215)
                     /locus_tag="VFB52_07055"
     CDS             complement(4370..5215)
                     /locus_tag="VFB52_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO06129.1"
                     /translation="MDVSIGLPNAVPGTTGEQLTEWAKRAEARGFKSLGTIDRIAYDN
                     YEPLVALSAAAAVTERIGLTTSVLLAPLRESAAAVAKQTISLQALSNGRLTLGIGIGG
                     REDDYATAEVEMGERAETIDQMLARIIELWDADHVGPSTVGPPRLLVGGGVDASYARA
                     AKYGDGWIAGGLPPEAFAEAAAKVKEEWEKAGREDSPRLEGLAYFSLGDEAEANANAY
                     LKDYYAWLGEETATFIANSAAKDAETVQGYLAAFEGVGCDELILFPSSSDPAQVDLLA
                     DAAGL"
     gene            complement(5257..5712)
                     /locus_tag="VFB52_07060"
     CDS             complement(5257..5712)
                     /locus_tag="VFB52_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO06130.1"
                     /translation="MSDTAVAGERVELAADFVARFADFWSAPAPGRLDALLAPEVRLV
                     APLTPTTRTLADGKRAFASLLRLSPDLTAEVHSWGATPAGVMIDFTLNGSVSGVGLRW
                     RAVDRIALRDDGLASERVSHFDSLPLLLTALRHPRTWPRFLYSQAKRLG"
     gene            complement(5709..6314)
                     /locus_tag="VFB52_07065"
     CDS             complement(5709..6314)
                     /locus_tag="VFB52_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06131.1"
                     /translation="MSATAERNPASPQEGSKGEQTRAAILERAVDLASAEGLEGLTIG
                     RLAAELQMSKSGLFAHFGSKQELQLATVEAAAARFSAAVIEPSLAAPDGAPRLRALNE
                     RYLDHLGLYSGGCFWGATSAEYDDRPGPVRDAIAAALEAWIAELERQAAVAGVEDPAR
                     FAFELYALIMGANARFRLSGDPQVFEHARAALDRLMEELPK"
     gene            6378..6773
                     /locus_tag="VFB52_07070"
     CDS             6378..6773
                     /locus_tag="VFB52_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06132.1"
                     /translation="MSNYAVKEIDSMEAVYLGAFKRARAELGVESFGMAVIDLPPNFD
                     QYPEHDHSHDQQEEVFLAMRGSGEIEIEGERFPLDDNHIARVAWQAKRKLLSGPEGIR
                     VLALSGCPGKLYEAPEVSKLGEPDPMATS"
     gene            6807..7745
                     /locus_tag="VFB52_07075"
     CDS             6807..7745
                     /locus_tag="VFB52_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZO06133.1"
                     /translation="MLRKLPLVLVSLALLALTVAAPAGATVAPGDPDDPVVVVVGDVV
                     VPRGEAVEGVYLMSGDARIAGRVDGDVIVLSGDVVVSGQVSGDLFTASGTARLLPGAE
                     VEGDVTYSDHHPTVSFDAIVGGDVKEESLPDLGGALPLLGGFLIWLAFGVSQVLLGLF
                     LILIAPRAADAVHARSRERVGPTIGIGVAMAIALPVVAVVAAITVLGLPLAFILLLAL
                     LPLWSVAYLASAWALGRRLVGAPRQRPLSFLAGLGIFWAIGLVPIVGALFTLVATVFG
                     LGLIGAAIGAARDPKPKPDPDQPPPPPPGYAQIPGS"
     gene            complement(7899..8270)
                     /locus_tag="VFB52_07080"
     CDS             complement(7899..8270)
                     /locus_tag="VFB52_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06134.1"
                     /translation="MDAAAARLPHIDEHATVIAADPTATWAAVLQTAEGLPSRGFRVA
                     SKDPEKELALTGHHPFSRYALVFRLDDLGEGRTRLRAETRAVFPKLRGRAYRALVIGT
                     RMHVLVTRRLLGATKRRAERP"
     gene            8377..10521
                     /locus_tag="VFB52_07085"
     CDS             8377..10521
                     /locus_tag="VFB52_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZO06135.1"
                     /translation="MTPTATLFDADAGSYTGTIEVLGVIFRADDDGYAVLEVQEAGSG
                     EGFALVGPVAHLGEGDRAEVNGEWQTHSRYGRQLRAQGALPLDPADREGQIAYLTTLR
                     HIGKRRAERLVDEHGEEVLAKIAANPHAIFSGLKGVSVDQAAAATESWHASRAVRDLH
                     VQLAPHGLAHLAAPIHARYAERSMTILHEDPYRLTEVPGVGFARADKIALAADVPPES
                     SRRAQAAAVFALAEAEQQGNSFLPLPELTRQTARLIGLAPDPDVLVEAPGLVVDEERV
                     YREPTLASEIAVAETLRSRAAQPPHFDHDPGEEPGELADGSRLTDEQWAAVRGAFGAR
                     VSVLTGGPGVGKTLCTKGIVAEAEAAHATLALCAPTGRAARRMEETTGHEAMTIHRLL
                     EWMPGREPAYRPGHPLPVDLVIVDEASMLNLRLVEVLLNGLAESTHVVFVGDADQLPP
                     IGAGKPFEDLIDSGAVPVVRLTQIFRQAARSMITTAAHEINQGRPPHREPGPDQDHDF
                     FFIERATPERALETVVEVVAERAPAGFGVDPIRDVQVLAPMYRGPVGIDALNERLQAR
                     LNPEGKRALSDRFRIGDRLIQTRNSHELGLMNGSIVFLREDNPDEETIRVDTDEGGSL
                     LIPYGETATLKLAYAISVHKAQGCEVPVVVGVCHRSHSRMLTKPLLYTAITRARNSCV
                     LVGDPGALEAAVRRDDAGARHSGLAARLDEYS"
BASE COUNT         1599 a         3730 c         3854 g         1549 t
ORIGIN      
        1 cgagcgagcc gagtaggcgg acgagcacgg cgagcatgtt agatcgggga accccgagaa
       61 atcaccgcag ttgcggtcgc cgctcaggcg cgcggcaaaa cgcgcatccg caggccgcct
      121 ttcggcgaga gcgtcggcat ctggcggatc gtcatcgtgc ggccgggcag ggcgtcgagg
      181 cgcagctgct ggagcagcat cgaggcgacc agcttgacct cgatctggcc gaagcgtttg
      241 ccgatgcaga cacgctggcc gccgccgaag gggacgtagg cgccgcgtgg cagcgccgcc
      301 ttgcgctcgc gggtgaaacg ctcggggatg aacgcctcgg gatcggggaa gaccgacggg
      361 atccggtggc tggcccagga gcagtagttg gcgtaggcgc ctcgaggcac ggcgtagccg
      421 ccgaactcga aatcgcggac tgcgcggcgg gggccgatcc aggccggcgg gtagaggcgc
      481 aggacctcgt cgaggaccat gtcgaggtag ggcatctcgc gctccagctg gtcgatcgtc
      541 ggcgggcggc cgccgagcac ctcgtcctgc tcggcgcaga gcttctcgag cacgtcgggg
      601 tggcgggcga gctcgtgcag catgaaggtg acggtcgagg tcgaggtgtc gtgcccggcg
      661 aacatcaggg tcatcacctg gtcgcggacc tcgcgatcgc tgaacgagtc gccgtcctcg
      721 ccgcgcacgt tgaccagcag gctgaggatg tcgcggcgct cggggtcggg cttggcgcgg
      781 cggcgagcga tctcgccgaa gacgatctcg tcgagcacct cgcgcgaggc attcatcttg
      841 cgccagggcg agccgggtcc acgcagcagc cgcagggcga agtcgatccc gtagaagccg
      901 agcgcgcgct cgaagtgctc ggccgcggcg gcacccttgc cggcctcgtc gggatcgagc
      961 ccgagcagcg cccgcatcgc gatccgcatc gccaggttgc gcatccagtc gtagacgtcg
     1021 accacgccgc cgacctgcaa cccggcgatc gcgacttccg cctcggcggt catcgccgcc
     1081 gtcgcgatcg cgacctgctc gcggtggaag gccggcatca tgatcgcccg ggcccgatcg
     1141 tggaaggcat cgtcgatcgt cagcaggccg tcgccgagca gcgggatcag gtcgccgaag
     1201 ctcgactcac gccagtggaa gttctccggg tgggcgacgg tgacgaagtg gttggcctcg
     1261 gggccgagca tgaagatgac ccgcgtgtgc agcaggcgca tgctgaagat cggcccgaaa
     1321 tcttcgtagg cgccgagcag cagcggcagc gggtcgtggg cggcctggtg ggtgcgggcg
     1381 aagctgaagt cggtcgggcc gggtgggaac tgccccctcc cccgccactc ggcagcgacg
     1441 tcttcggcga cccggcgaac cgggttcgcc gacatctcct tcggccgctc gagcggggcg
     1501 gcggtgtcga tcgtggccac gtccgaatcc taactggctg ggcagccagt tttcttcgga
     1561 ccgactggtt tgccctatcg ttgcggttcg aattgggaaa ttctgtgttc tcaattcagg
     1621 tagggaatgt ctttgagaaa gggagagaat tgagaatgca tgggaaactg cggctgagcc
     1681 tgctcgcggc gctctgtctg ctgggagtct cggtcatcgt cgccagcgcc accgccgaga
     1741 cggcgaccgt tggcaacatc aagttcaccg ccgacggcgg cttcagtccg aaggcgctgc
     1801 cgaagaaaac cttcgcgccg atcgcgctga ccgcgaaggg gaagatcgag acgaaagacg
     1861 gcacccaccc gccggcgctg aaagaagtgc tgctggaaac cgacaaaaac ggcgcgatcg
     1921 acgtcaaagg ctatcccgcc tgcacgtcgg gcaaactgca gtcgcagacg accgcgaacg
     1981 ctgaaaaagc ctgcaaaggc acgcttatcg gcacgggcaa gaccacggtc gagatcgaac
     2041 tggccgacca gccgccgatc gacgcgaaga gcaaattgct ggtcgtcaac ggcggcgtca
     2101 agggtggcgt gaccaccctg ttcatccacg cctacctcac ccaaccggtc ccggccgcgg
     2161 tcgtgaccac ggtgaaaatc aagaagatca acaaaggccg ctacggaacc ctctcggtcg
     2221 cctcgatccc ggtgatcgcc agcggcgccg gctcggtgac cgacttcgaa ctcaccgtgg
     2281 acaaaaagtt cacttacaaa ggcaagcaga agagcgtcct cacggcgaaa tgccctgacg
     2341 gcaagctcca ggccaacgca acggccatct tcagggacgg cacccgcgtc tcgcaggact
     2401 tcgtccgccc ctgcaccggc aagggctgat ccgaagctga ggttggacgg cccgggccac
     2461 gcgcccgggc cgttcgccta ggccgctgcc ttggcggcga gctgcccgca ggcggcgtcg
     2521 atgtcgcggc cgcgggtgag acggacggtg gccggcaggc gctgctcggc gaggatcgag
     2581 cggaagcgct cgatccgctc cggtgaggag ccgtcgtagg caccggtcgg gttgtagggg
     2641 atcaggttga ccttgtagac ccgcgggtcg agcagggcag cgagttcgcg cgcgtgctcg
     2701 ggcaggtcgt tgaccccgtc gagcatcacg tactcgacga agaccttgcg gcggcgggcg
     2761 ccgcggtagc gatcgcaggc ggcgagcacg tcggccagcg ggtagcggtc gttgaccggc
     2821 atcagttgcg agcgcagccc gtcgttggga gcgtgcaggg agagcgccag ggcgatcggc
     2881 atctcgcact cggcgaggcg gtcgatcccc ggcacccagc cgaccgtcga gatcgccgtg
     2941 cgccggtggg tgacgccgag gtcgggcagc cgttcgcagg cggcgagcac gttgtcgatg
     3001 ttcatcgtcg gctcccccat gcccatgaag acgacgtggt cgatcgcttc ggcgcggcgg
     3061 aagtgcagcg cctggtcgag gatctcgtcg gcgccgaggt tgcggccgaa cttcatctgg
     3121 ccggtggcgc agaaggtgca ggtcagcggg cagcccgact gcgaggagac gcagaccgag
     3181 cggcggccgt cgcggtagcg catcagcacc gcctcgatcg ggcgcccgtc ggcggtctgg
     3241 aacagcgtct tcacggtgcc gtcgtcggag tgggcctcgg cctcgaccgc gagggtggag
     3301 agcggcacgg ccgcctcgag gcgctcgcgc aaagctgccg gcaggttggt catctccgcg
     3361 taggagcgcg ccccgcgcgc gacccactcc cagatctggg aggcgcggta gccgggctcg
     3421 ccggtctcgg cgagggtgct ctcgagaaga tcgcggtcca cgtgtcgagt gtacggacgc
     3481 agaagttccc ggtatgcggg aacttctgcg cctcagccgt ggatcttctc gccgcgctcg
     3541 agcatcgcga cgaatttgcg cagccgtcgc tcgcgcgtct ccggtttctt cgcctcgccg
     3601 agccgataga cgatcgcgta gcggttggcg ctgtcgagct cggcgaagaa cgcggccgcc
     3661 gccgggttgg cgtcgagctc gcgctgcagg tcgtccggca ccttggccgt gcgctgaccc
     3721 tcgtaggcgg cctcccaacg accatcggcg cgggcggcct cgatctccgc catccccgcc
     3781 ggccgcaccc gctcgtccgc gatcagcgcc tcggctttct cgcggttgat cttcgaccag
     3841 cgcccgcgcg ggcggcgcgg cgtgaagcgc tgcaggaagt gggtcgcatc gagggcacgg
     3901 cgctggccgt cgatccaacc gaagcagagc gccagctcga ccgcctccgc gtaggtcacg
     3961 ctcggctccg gcgcgccctt cttggcgatc tgcagccaga tcccgccggg ctcggcgtgg
     4021 tggtcctcca gccaggcctc gaactcgtcc gcgctcgcga acagcagcgt cggcagctgg
     4081 tcagccatcg tcgccccccg acagggcggc gagcagctcg tcctggccga cgccctcgac
     4141 ctcgatcagc ttctcccgtg acttggcgcc gcgggcgacg ccgacccggc tcggcgccac
     4201 gccgacccgc ttggcgatca gccggcagag cgccttgttc gccttgccgt cgaccggtgg
     4261 cgccgcgacg cgggcctgga gcacgccgtc ctccaggccg agcagctcgt cggcgtgtcc
     4321 gcgggggcgc agccggaccg cgatccgcgc ccccgccacc accgtgcctc tagagcccgg
     4381 cggcgtcggc cagcaggtcg acctgggccg ggtcgctgga gctcgggaag aggatcagct
     4441 cgtcgcagcc gacgccctcg aaggcggcca ggtagccctg caccgtctcc gcgtccttgg
     4501 cggcgctgtt ggcgatgaag gtcgcggtct cctcgccgag ccaggcgtag tagtccttga
     4561 ggtaggcgtt ggcattcgcc tcggcctcgt cgccgagcga gaagtaagcg aggccctcga
     4621 ggcgcgggga atcctcgcga ccggccttct cccactcctc cttgaccttc gcggcggcct
     4681 cggcgaacgc ttccggcggc aggccgccgg cgatccagcc gtcgccgtac ttggcggccc
     4741 gggcgtagga ggcgtcgacg ccaccgccga ccagcaaacg cgggggaccg accgtcgagg
     4801 gaccgacgtg atcggcgtcc cagagctcga tgatccgggc cagcatctgg tcgatcgttt
     4861 cggcgcgctc gcccatctcg acctcggcgg tcgcgtagtc gtcctcgcgg ccgccgatcc
     4921 cgatccccag cgtgagcctt ccgttcgaga gcgcctgcag gctgatcgtc tgcttggcga
     4981 ccgcggcggc actctcgcgc agcggcgcca gcagcaccga ggtggtgagc ccgatccgct
     5041 cggtcaccgc agcggcggcc gagagtgcga ccagcggctc gtagttgtcg taggcgatcc
     5101 ggtcgatcgt gccgaggctc ttgaagccgc gcgcctcggc gcgcttggcc cactcggtca
     5161 gctgctcacc ggtggtaccg gggactgcgt tgggaagtcc gattgaaacg tccatataga
     5221 agctccttgg ggggttgcga ggccgacgag cgaaccctag cccaaccgtt tcgcctggct
     5281 gtacagaaat cgtggccagg tgcgcgggtg gcggagcgcg gttagcaaca gcgggaggct
     5341 gtcgaagtgg gagacgcgct cgctggcgag gccgtcgtcg cggagcgcga tgcggtcgac
     5401 cgcacgccag cgcaacccaa cgccgctgac gctgccgttg agcgtgaagt cgatcatcac
     5461 accggccggg gtcgcacccc aggagtggac ctcggcggtc aggtcgggtg agagccggag
     5521 caagctggcg aaggcgcgct tgccgtcggc gagcgtgcga gtggtcgggg tgagcggcgc
     5581 gaccaggcgc acctcgggcg ccagcagggc atcgaggcgg ccgggggccg gagcgctcca
     5641 gaagtcggcg aagcgagcga cgaagtcggc cgcgagctca acccgctcgc ccgcgacggc
     5701 ggtgtcgctc acttcggcag ttcctccatc aggcgatcga gcgcggcgcg ggcgtgctcg
     5761 aagacctgcg ggtcgccgct cagccggaag cgcgcgttgg cgcccatgat cagggcgtag
     5821 agctcgaagg cgaagcgggc tgggtcctcg acgccggcga ccgcggcctg gcgctcgagc
     5881 tcggcgatcc aggcttcgag cgccgcggcg atcgcgtcgc ggacggggcc gggacggtcg
     5941 tcgtactcgg ccgacgtggc accccagaag cagccgcccg agtagaggcc gaggtggtcg
     6001 aggtagcgct cgttcagcgc ccgcaggcgc ggcgccccgt cgggggccgc cagcgagggc
     6061 tcgatcaccg cggcgctgaa gcgggcggcg gcggcttcga cggtggccag ctgcagctcc
     6121 tgcttggagc cgaagtgggc gaagaggccg ctcttgctca tctgcagctc ggcggcgaga
     6181 cggccgatcg tcagcccctc gagcccctcg gcggaggcga ggtcgacggc gcgctcgagg
     6241 atcgcggcgc gcgtctgctc ccccttgctg ccctcctgcg gggaggccgg gttgcgctcg
     6301 gcggttgcgc tcacggccga aagcatatag aaaagcacga acgttcgttt tattttctaa
     6361 ccggaggggg aacagcggtg agcaactacg cggtgaaaga gatcgactcg atggaggccg
     6421 tctacctggg cgccttcaag cgggcgcggg ccgagctggg cgtcgagtcg ttcgggatgg
     6481 cggtgattga cctgcccccc aacttcgacc agtaccccga gcacgaccac agccacgacc
     6541 aacaggagga ggtcttcctg gcgatgcgcg gcagcggcga gatcgaaatc gagggcgagc
     6601 gctttccgct cgacgacaac cacatcgccc gggtcgcctg gcaggcgaag cgcaagctcc
     6661 tctccgggcc cgagggaatc cgcgtgctcg ccctcagcgg ctgccccggc aagctctacg
     6721 aggcgcctga ggtgagcaag ctgggcgagc cggacccgat ggcgaccagc taggacggca
     6781 gccccggcct tgaataggct ggccgggtgc ttcggaagct cccgctcgtg ctggtctcgt
     6841 tggcgctcct ggcgctgacg gtcgcggcac cggcgggggc aacggtcgcg ccgggagatc
     6901 cggacgaccc agtggtggtc gtcgtcggcg acgtcgtcgt ccctcgcggc gaggcggtcg
     6961 agggcgtgta cctgatgagc ggcgacgccc gcatcgccgg ccgggtcgac ggcgacgtga
     7021 tcgtgctcag cggcgatgtc gtcgtctccg gccaggtcag cggcgacctc ttcaccgcca
     7081 gcggcaccgc ccggctcctg ccgggcgcgg aagtcgaagg agacgtcacc tactccgacc
     7141 accaccccac cgtctccttc gacgcgatcg tcggcggcga cgtcaaggaa gagagcctgc
     7201 ccgacctcgg tggcgccctg ccgctgctcg gcggcttcct gatctggctc gcgttcggcg
     7261 tctcgcaggt gctgctcggc ctgttcttga tcctgatcgc cccgcgggcc gccgacgcgg
     7321 tccacgcgcg ctcgcgcgaa cgggtcgggc cgacgatcgg gatcggcgtg gcgatggcga
     7381 tcgcgttgcc ggtggtcgcg gtggtcgccg cgatcaccgt gctcggcctg ccgctggcct
     7441 tcatcttgct gctggccctg ctgccgctct ggtcggtcgc ctacctggcc agcgcctggg
     7501 ccctgggacg acggctggtc ggagcgccgc gccaacgccc gctctccttc ctcgccggcc
     7561 tcgggatctt ctgggcgatc ggcctggtcc cgatcgtcgg ggccctgttc accctcgtcg
     7621 cgacggtctt cggcctcggc ctgatcggcg cggctatcgg ggcggctcgg gatccgaagc
     7681 cgaagcctga cccggaccag cctccgcccc ctcctccggg ctacgctcaa atcccgggca
     7741 gctgagcacc gggatccgcg ggtaacgcgg gtactcgggg tcctcgcgcg agcgccgaca
     7801 gagcgagaag accgagccgc gccggttcgg gacgagctgt tgatggcggc actggtcgca
     7861 gagcccgacc ggcgggcggg acgggcgcgg cggcgcgctc acgggcgctc ggcgcgtcgc
     7921 ttggtcgcgc cgagcaggcg gcgggtgacg agcacgtgca tccgggttcc gatcacgagc
     7981 gcacgatacg cgcggccgcg cagcttcggg aagacggccc gggtctcggc ccgcagccgc
     8041 gtccgcccct cccccagatc gtcgagccgg aagaccagcg cgtagcgcga aaaggggtga
     8101 tggccggtca gcgccagctc cttctccggg tccttgctcg ccacccggaa gccgcgggaa
     8161 gggagcccct ccgcggtctg cagcaccgcc gcccaggtcg cggtcgggtc cgcggcgatc
     8221 acggtcgcgt gctcgtcgat gtgaggcaag cgcgcggcgg cggcgtccat atggtgatgc
     8281 tacgcgcatc gacccgatcc atacggtgtc gtatgtcacg gaaaatgcgg gtctggaggc
     8341 gatcggtccc gcctccccgc tggctagctt tggtgggtga ccccgaccgc caccctgttc
     8401 gacgcagacg ccggctccta caccggcacg atcgaggtgc tcggggtgat cttccgcgcc
     8461 gacgacgacg gctacgcggt gctcgaggtg caggaggcgg gcagcggcga aggcttcgcc
     8521 ctggtcggcc cggtcgccca cctcggcgag ggcgatcgtg ccgaagtcaa cggcgagtgg
     8581 cagacccact cgcgctacgg ccgccagctg cgcgcccagg gggcgctacc gctcgacccc
     8641 gccgaccgcg agggccagat cgcctacttg accacgctgc gccacatcgg caagcgccgc
     8701 gccgagcggc tggtcgacga gcacggcgag gaggtgctcg cgaagatcgc cgccaacccg
     8761 cacgcgatct tctccgggct gaagggcgtc agcgtcgacc aggccgcggc ggcgaccgaa
     8821 tcgtggcacg ccagccgtgc ggtccgcgac ctgcacgtgc agctcgcccc gcacgggctc
     8881 gcccacctcg cggcgccgat ccacgcccgc tacgccgagc gctcgatgac gatcctgcac
     8941 gaggacccct accggctgac cgaggtgccc ggggtcggct tcgcccgcgc cgacaagatt
     9001 gcgctcgccg ccgacgtgcc gccggagtcc agccgccgcg cccaggccgc cgctgtcttc
     9061 gccctggccg aggccgagca gcagggcaac agcttcctgc cccttcccga gctgacccgc
     9121 cagaccgccc gcctgatcgg cctcgccccc gaccccgacg tgctggtcga ggcgccgggg
     9181 cttgtcgtcg acgaggagcg cgtctaccgc gagccgaccc tggccagcga gatcgccgtc
     9241 gccgaaaccc tgcgctcgcg ggccgcgcag ccgccgcact tcgaccacga tcccggcgaa
     9301 gagccgggcg agctcgccga cggcagccgc ctcaccgacg agcagtgggc ggccgtgcgc
     9361 ggcgccttcg gcgcccgcgt ctcggtcctc accggcggtc ccggcgtcgg caagaccctc
     9421 tgcacgaagg ggatcgtcgc cgaggccgaa gcggcgcacg cgacgctcgc gctctgcgcg
     9481 ccgaccggcc gcgcggcgcg gcggatggag gagacgaccg ggcacgaggc gatgacgatc
     9541 caccgtctgc tcgagtggat gcccgggcgc gagccggcct accggcccgg ccacccgctg
     9601 ccggtcgacc tggtgatcgt cgacgaggcc tcgatgctga acctgcgcct ggtcgaggtg
     9661 ctgctgaacg ggctcgccga gtcgacccac gtcgtcttcg tcggcgacgc cgaccagctg
     9721 ccgccgatcg gcgccggcaa gccgttcgag gacctgatcg actcgggggc ggtaccggtc
     9781 gtgcggctga cccagatctt ccgccaggcg gcgcgctcga tgatcaccac cgccgcccac
     9841 gagatcaacc agggacgccc gccccaccgc gagcccggcc ccgatcagga tcacgacttc
     9901 ttcttcatcg agcgggcgac gccggagcgg gcgctggaga cggtggtcga ggtggtcgcc
     9961 gagcgagccc cggccgggtt cggggtggac ccgatccgcg acgtgcaggt gctggcgccg
    10021 atgtaccgcg ggccggtcgg gatcgacgcg ctcaacgagc gtttgcaggc gcgcctcaac
    10081 cccgaaggca agcgagcgct ctcggaccgc ttccggatcg gcgaccggct gatccagacc
    10141 cgcaactcgc acgagctggg gttgatgaac ggctcgatcg tcttcctgcg cgaggacaac
    10201 cccgacgagg aaacgatccg ggtcgacacc gacgagggcg gctccctgct gatcccttac
    10261 ggcgagacgg cgacgctgaa gctcgcctac gcgatctccg tccacaaggc gcagggctgc
    10321 gaggtgccgg tcgtggtcgg ggtctgccac cgctcgcact cgcggatgtt gaccaagccc
    10381 ctgctgtaca ccgcgatcac ccgcgcccgc aacagctgcg tgctggtcgg cgacccgggc
    10441 gcgctggagg cggcggtgcg acgcgacgac gccggcgctc gccactcggg cctggcggcc
    10501 cggttggacg agtactcctg atcgccgagg gaagcctgcc caacgttcgt ccgtgcctgg
    10561 gaatagtgtc ctgggcgtgc gcatatactc aaaattgacg atgaataaac gcatcgttac
    10621 ccctctcccg ctagtcctct tggccctcct ggcggcggtc gctttccccc aagcggccgc
    10681 cgccggggcc aagagcccgg gagtcgccct gcccaaaacg aaggtccagg cg
//
LOCUS       DASRIG010000258         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3244224, whole genome shotgun sequence.
ACCESSION   DASRIG010000258 DASRIG010000000
VERSION     DASRIG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244224"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFB52_07090"
     CDS             <1..1154
                     /locus_tag="VFB52_07090"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HZO06136.1"
                     /translation="IGLLTFLFLLLRTFVQAEPPSAERRRRAEEIVRRWGDDTLDYFA
                     LRRDKSYFFSSDGHSLIAYLYVRGTAMVAADPIGPPADTARTIDEFLAFCAERGWRVA
                     FFAVRESDADLYKERGMHPIYLGDEAIVHPDRFTLEGGEMKAARAAVKHVSHSHDFEL
                     IAETEASQELIDELNEISAEWRDGSPERGFTMELGEEVEGTNPDFVLAIAKERGGRVA
                     GFLRFVPVYGSEPGYSLDLMRRRPDSANGLTEYLISEAALALGSRGFKRLSLNFAAWG
                     RLLDSAEGAGLSGKLQRLMAKGLNPFFQIQSLRDFNQKFDPEWVPRSIVIDDVADLPR
                     VAMLYASVEGFLDVPVLSRVLEAPLRQRVEAEAAEPVPEPVGPPGDPPA"
     gene            complement(1054..2178)
                     /locus_tag="VFB52_07095"
     CDS             complement(1054..2178)
                     /locus_tag="VFB52_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06137.1"
                     /translation="MRFHPLQRAARNANVGSLHLAFFVTAIVTELCIRTQLWLTNYPQ
                     LGGHGLHIAHLLWGGLFMVIALAILLSFLGRGARQAAAIVAGVGFGFFIDELGKFITA
                     DNNYFFKPAAGIIYLVFIALYLSSRAFQNYRGLTEAEKVRNAVELIGEATQGPFKAEY
                     RERALALLAEVPAANPLRAPLVELAEGIDTTPNPNPPWYRRFADGVAARYLRWSEQSW
                     FHGAVIGVFAVWAAASILAIVGLIFSAGFAGDAARVGFEEDSVGHLNFVNWATLVSTA
                     VSTSFVVVGLSRLVRGDHDDAYLWLTRALLVSIFITRVFVFVESQFGGVFGIGVDVLL
                     LISVRLMAKQEGRREARQAPAQALPPRPRPAGAAAPPAPG"
     gene            complement(2175..>2537)
                     /locus_tag="VFB52_07100"
     CDS             complement(2175..>2537)
                     /locus_tag="VFB52_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HZO06138.1"
                     /translation="AIGGGVVAGLVPLSILGELISIGTLLAFIIVSFGVLRLRRTHPD
                     LERPFRVPALPLVAGTAIAASVALMATLPGDTWIRLAVWTVAGLTIYFFYARDHSKEK
                     LDAVLAQATAPAAEEGKA"
BASE COUNT          414 a          849 c          930 g          344 t
ORIGIN      
        1 tgatcgggct gctcaccttc ctgttcctgc tgctgcggac cttcgtccag gccgagccgc
       61 cgagcgccga gcgccgccgc agggccgagg agatcgtgcg ccgctggggc gacgacacgc
      121 tcgactactt cgccttgcgc cgcgacaaga gctacttctt ctccagcgac gggcactcgc
      181 tgatcgccta cctctacgta cgcgggaccg cgatggtcgc cgccgacccg atcgggccgc
      241 ccgcggacac ggcgcgcacg atcgacgagt tcctcgcctt ctgcgccgag cgcggctggc
      301 gggtcgcttt cttcgccgtg cgcgagtccg acgccgacct ctacaaggag cgcggcatgc
      361 acccgatcta cctgggcgac gaggcgatcg tgcatcccga ccggttcacg ctcgagggcg
      421 gcgagatgaa ggcggcgcgg gccgcggtca agcacgtctc gcacagccac gacttcgagc
      481 tgatcgccga gaccgaggcg agccaggagc tgatcgacga gctcaacgag atcagcgccg
      541 agtggcgcga cggctcgcca gagcgcggct tcacgatgga gctcggcgag gaagtcgagg
      601 ggaccaaccc cgacttcgtg ctcgcgatcg ccaaagagcg cggcggccgg gtcgccgggt
      661 tcctgcgctt cgtccccgtc tacggctccg aacccggcta ctcgctcgac ctgatgcggc
      721 gccggcccga ctccgccaac gggctgaccg agtacctgat ctccgaggcg gccctggcgc
      781 tcggctcgcg cgggttcaag cggctctcgc tcaacttcgc cgcctggggc cggctgctcg
      841 acagcgccga aggcgcgggc ctcagcggca aactgcagcg gctgatggcg aaggggctga
      901 acccgttctt ccagatccag agcctgcgcg acttcaacca gaagttcgac cccgagtggg
      961 tgccgcgttc gatcgtgatc gacgacgtcg ccgacctgcc gcgggtggcg atgctctacg
     1021 ccagcgtcga gggcttcctc gacgtgccgg tgctcagccg ggtgctggag gcgccgctgc
     1081 gccagcgggt cgaggccgag gcggcagagc ctgtgccgga gcctgtcggg cctcccggcg
     1141 accctcctgc ttagccatca gccgcaccga gatcagcagc agcacgtcga cgccgatccc
     1201 gaagacgccg ccgaactgcg actcgacgaa gacgaagacg cgggtgatga agatcgagac
     1261 gagcagcgcc cgggtcagcc agagataggc gtcgtcgtgg tcgccgcgga ccaggcggct
     1321 gaggccgacg acgacgaagc tggtcgagac cgcggtcgag acgagggtcg cccagttgac
     1381 gaagttgagg tggccgaccg agtcctcctc gaagccgacg cgggcggcat cgccggcgaa
     1441 cccggcggag aagatcaggc cgacgatcgc caggatcgag gccgccgccc agaccgcgaa
     1501 gacgccgatc acggcgccgt ggaaccagct ctgctccgac cagcgcaggt agcgggcggc
     1561 gacgccgtcg gcgaagcggc ggtaccaggg cgggttcggg ttcggggtcg tgtcgatccc
     1621 ctcggcgagc tcgaccagcg gcgcacgcag cgggttggcg gccggcacct cggcgagcag
     1681 ggcgagcgcg cgctcgcggt attcggcctt gaacggcccc tgcgtcgcct cgccgatcag
     1741 ctcgaccgcg ttgcgcacct tctcggcctc ggtcagcccg cggtagttct ggaaggcgcg
     1801 cgagctgagg tagagcgcga tgaagaccag gtagatgatc cccgcggccg gcttgaagaa
     1861 gtagttgttg tcggcggtga tgaacttgcc gagctcgtcg atgaagaagc cgaagccgac
     1921 gccggcgacg atcgcggccg cctggcgggc gccgcggccg aggaagctga gcaggatcgc
     1981 cagcgcgatc accatgaaca ggccgcccca gagcaggtgg gcgatgtgca ggccgtggcc
     2041 gccgagctgc gggtagttgg tcagccagag ctgggtgcgg atgcagagct cggtgacgat
     2101 cgcggtgacg aagaaggcga ggtggagcga accgacgttg gcgttgcggg cggcacgctg
     2161 cagcgggtgg aacctcacgc cttgccctcc tcggcggcgg gcgcggtggc ttgcgccagc
     2221 acggcgtcca gtttctcctt gctgtgatcg cgggcgtaga agaagtagat agtcaggccg
     2281 gcgacggtcc agaccgcgag ccggatccag gtgtcgccgg gcagggtcgc catcagcgct
     2341 accgaggcgg cgatcgcggt cccggcgacc agcggcagcg ccggcacccg gaagggacgc
     2401 tccaggtccg ggtgggtgcg gcgcagcctc aggaccccga aggagacgat gatgaaggcg
     2461 agcagggtgc cgatcgagat cagttcgccg aggatcgaga gcgggaccag gccggcgacg
     2521 acgccgccgc cgatcgc
//
LOCUS       DASRIG010000259         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2994737, whole genome shotgun sequence.
ACCESSION   DASRIG010000259 DASRIG010000000
VERSION     DASRIG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2994737"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFB52_07105"
     CDS             complement(<1..368)
                     /locus_tag="VFB52_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06139.1"
                     /translation="MASATEFPLDLDRMAVDPYPVYAQMRREAPVAFVPQLNLFMVTR
                     WSEVEAVASGRETWGSPAGLDRLNRTFGEPNVLTANGEEHAMQRVGIDAVLRPRAVNT
                     YVEELIRPIATELIEGLRPAG"
     gene            complement(371..1357)
                     /locus_tag="VFB52_07110"
     CDS             complement(371..1357)
                     /locus_tag="VFB52_07110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZO06140.1"
                     /translation="MAGLPSWMVDPAPAPGGETIEMRVAAKRQLSEGVVALELVPPEG
                     EDAPVWEPGAHVDLHLPKGMARQYSLCGDPADRDALLVAVLREEEGRGGSRHLHDVTA
                     VGDLLPVGGPRNNFALEPSPRYLFIGGGIGITPLVPMIAAARASGSEWSLLYGGRTRG
                     SMAFLDELSADPRVTVVPEDERGMLDLAGALAEVLPETLIYCCGPEPLLHAVEETSAH
                     WGAEALRLERFKARDDLPTDPGTAFEVVLDRSGLSLTVQPGQSILDAVAAAGVAVPSS
                     CQEGTCGTCETLVLEGEPEHRDSVLTPADRARNDCMMICISRARSERLVLDL"
     gene            complement(1360..2550)
                     /locus_tag="VFB52_07115"
     CDS             complement(1360..2550)
                     /locus_tag="VFB52_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO06141.1"
                     /translation="MDEELRHLSCYTTPPARTLELFEAARAGGCPVAHSEEHDGFHLL
                     LAYEDVRKGMADHELFSSKPQVLRPMLPRKPIPALEMDPPQHQHWRALFNQAVTARTS
                     GVLEPQVRADINRHIDAFIGRGSAELVNDLCEPVPAEAICHLVGVDEELVPVVREKAI
                     AMFAAMGEPEEFGRLQAEFGEVTVGEVHKRKAEPRDDYLTYLATVEVEGRELDDDDYV
                     VLLAAFLGAGHHSTTSAMSSLIYEVFSDEELKAKLIADPEAIPTAVEETLRLHPPFYG
                     FFRRTTEDTEVEGVAIPEGCDVYMAWAAANRDPDQFPEPDEFKLDRGRNRHMSFGFGV
                     HTCPGAPLARMELRVAIEELLRRLPDLKIEIDEPQYEFGGGDYAFLVELPVSFAPQTA
                     VASG"
     gene            complement(2741..>4043)
                     /locus_tag="VFB52_07120"
     CDS             complement(2741..>4043)
                     /locus_tag="VFB52_07120"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HZO06142.1"
                     /translation="LRLPSASVAPAIRTTIAGLTALAVLALAGGVASASERAHTSIGF
                     QPVPLSVATDAGGHVYLSNPNFSNTVEQYSSDGVLLAQLGNFPRSGSPLQPRAIATDA
                     AGNLYVADASSDLLAVLAPNGSPLRSWNADGRDLAVGGEGSVYVLGASEVLRFSPEGT
                     LLSKWGSSGEGDGQLGSPYGLDTSPSGLVYVADTYRNRIQVFTADGAFVTKWGSYGSD
                     DGEFIYPYGIATDAAGNVYVADTANARIQKFTSNGVFLGSFGGPGRGPGRFHTPTSVA
                     TDPAGFVYVADAGEPWPETGGARIQKFTADGQFVAEWGDIPDRRPARPRLSGSVGKRT
                     KKRFATFRFSSPRAGVHFQCRLSGERVPAKLRDWRACSSPQRYARLLPGRKLFHVRAL
                     SGSAKSGEARRSWSIVASRSSQSASALSSTVTASARTSTQT"
BASE COUNT          566 a         1379 c         1507 g          591 t
ORIGIN      
        1 ccggccggcc gcagcccctc gatcagctcc gtggcgatcg gccggatcag ctcctcgacg
       61 taggtgttga ccgcgcgcgg gcgcagcacg gcgtcgatcc cgacccgctg catcgcgtgc
      121 tcctcaccgt tggcggtcag cacgttgggc tcgccgaagg tgcggttcag ccggtcgagg
      181 ccggcggggc tgccccaggt ctcgcgcccg ctcgcgaccg cctcgacctc gctccagcgg
      241 gtgaccatga agaggttgag ctgcgggacg aaggcaaccg gcgcctcgcg cctcatctgc
      301 gcgtagaccg ggtagggatc gaccgccatc cggtcgaggt cgagcgggaa ctcggtcgcg
      361 ctcgccacgc tcagaggtcg agcacgaggc gctcgctgcg ggcccgcgag atgcagatca
      421 tcatgcagtc gttgcgggcc cggtcggccg gggtcaggac cgagtcgcgg tgctcgggct
      481 cgccctccag gacgagcgtc tcgcaggtcc cgcaggttcc ttcctggcag gaggagggca
      541 cggcgacgcc ggccgcggcg accgcgtcga ggatcgactg gcccggttgc accgtgagac
      601 tgagcccgga gcggtcgagc acaacctcga aggcggtgcc tgggtcggtg ggcaggtcgt
      661 cgcgggcctt gaagcgctcc aggcgcagag cctcggcacc ccagtgggcg ctggtctctt
      721 cgaccgcgtg cagcagcggc tccggcccgc agcagtagat cagcgtctcc gggagcacct
      781 cggcgagggc gccggcgagg tcgagcatgc cccgctcgtc ctcgggcacg accgtcaccc
      841 gcgggtccgc ggagagctcg tcgaggaagg ccatcgagcc gcgcgtgcgc ccgccgtaga
      901 gcagcgacca ctcgctgccg ctcgcccgcg ccgcggcgat catcgggacc agcggcgtga
      961 tcccgatccc gccgccgatg aacaggtatc ggggcgaggg ctccagggcg aagttgttgc
     1021 gcgggccgcc gaccgggagc aggtcgccga ccgcggtcac gtcgtgcagg tggcgggagc
     1081 cgccgcggcc ctcttcctcg cgcagcaccg caaccagcag cgcgtcgcgg tcggccgggt
     1141 cgccgcagag cgagtactgg cgggccatcc ccttgggcag gtgcaggtcg acgtgggcac
     1201 ccggctccca gaccggcgcg tcctcgccct ccggcgggac cagctcgagc gcgacgacgc
     1261 cctccgagag ctgccgcttg gcggcgaccc gcatctcgat cgtctcgccg cccggcgccg
     1321 gcgccgggtc gaccatccag ctcggcagac cggccatcgt cagccgctgg cgaccgcggt
     1381 ctgcggcgcg aagctgaccg gcagctcgac caggaaggcg tagtcgccgc cgccgaattc
     1441 gtactgcggc tcgtcgatct cgatcttcag gtcggggagg cggcgcagca gctcctcgat
     1501 agccacccgc agctccatcc gcgccagcgg cgcgcccggg caggtgtgga caccgaagcc
     1561 gaagctcatg tggcggttgc ggccacggtc gagcttgaac tcgtccggct ccgggaactg
     1621 gtcggggtcg cggttcgccg ccgcccaggc catgtagacg tcgcagccct cggggatcgc
     1681 gacgccctcg acctcggtgt cctcggtggt gcggcggaag aaaccgtaga agggtgggtg
     1741 cagacgcagc gtctcctcga ccgcggtcgg gatcgcctcg gggtccgcga tcagcttcgc
     1801 cttcagctcc tcgtcgctga acacctcgta gatcaggctc gacatcgccg aggtcgtgct
     1861 gtggtggccg gcgccgagga aggcggcgag caggaccacg tagtcgtcgt cgtcgagctc
     1921 gcggccctcg acttcgaccg tggccaggta ggtgaggtag tcgtcgcgcg gttcggcctt
     1981 gcgtttgtgg acctcgccga ccgtgacctc gccgaactcg gcctgcaggc ggccgaactc
     2041 ctccggctcg cccatcgccg cgaacatcgc gatcgccttc tcgcgcacga ccgggaccag
     2101 ctcctcgtcg accccgacca ggtggcagat cgcctccgcc ggcaccggct cacagaggtc
     2161 gttgaccagt tcggcgctgc cgcggccgat gaacgcgtcg atgtggcggt tgatgtcggc
     2221 ccggacctgc ggctccagca cgcccgaggt gcgtgcggtc accgcctggt tgaagagcgc
     2281 ccgccagtgc tggtgttggg gcgggtccat ctccagcgcc gggatcggtt tgcgcggcag
     2341 catcggccgc agcacctggg gtttcgagga gaacagctcg tggtccgcca tccctttcct
     2401 gacgtcctcg taggcgagca acaggtggaa gccgtcgtgc tcctcgctgt gcgcgaccgg
     2461 gcagccaccc gcccgcgccg cctcgaacag ctccagcgtc cgcgccggcg gggtcgtgta
     2521 acagctcagg tgccgcagct cctcgtccat gcgtctctcc tcttcgtggg cttacccaga
     2581 gaaactcata atgaatcgag ccgatgcctg tcaaccgtcc gcccagggtt gcaggcggtt
     2641 gcaagccggg gctaggaagg gttgagctgg gcgaagaaga cctgggcgcc gatccgggcg
     2701 aaggtctcgt gcgcggcgcg cagctgctcg cgcgcgtcgc tcaggtctgg gtgctcgtcc
     2761 tcgcgctcgc cgtcacggtg ctgctcagcg cgctcgcgct ctgggagctg cgcgaggcga
     2821 cgatcgacca ggaacgacgc gcctcaccgc tcttggcgga gcccgagagc gcacgcacgt
     2881 gaaagagctt gcggccgggg agcaagcggg cgtagcgctg cggcgaggag caggcgcgcc
     2941 agtcgcgcag cttggccggg acgcgctcgc cgctgagccg gcactggaag tggacgccgg
     3001 cgcgggggga gctgaagcgg aaggtcgcga accgcttctt ggtccgcttc ccgactgagc
     3061 cggagaggcg cgggcgggcc ggccgccgat ccgggatgtc gccccactcg gcgacgaact
     3121 ggccgtcggc ggtgaacttc tggatccggg cgccgccggt ctcgggccac ggctcgccgg
     3181 cgtcggcgac gtagacgaac ccggcggggt cggtggcgac ggaggtcggg gtgtggaagc
     3241 gacccggtcc gcggccgggg ccgccgaagg agccgaggaa gacgccgttt gaggtgaact
     3301 tctggatccg ggcgttggcc gtgtcggcga cgtagacgtt gccggcggcg tcggtggcga
     3361 tcccgtaggg gtagatgaac tcgccgtcgt cgctgccgta gctcccccac ttggtcacga
     3421 aggcaccgtc ggcggtgaag acctggatcc ggttgcggta ggtgtcggcg acgtagacca
     3481 ggcccgaggg gctggtgtcg aggccgtagg gagagccgag ctgcccgtcg ccttcgccgc
     3541 tcgatcccca tttggagagc agggtgcctt ccggggagaa acgcaagacc tcgctcgccc
     3601 cgagcacgta gacgctgccc tcgccgccaa ccgcgaggtc ccggccgtcc gcgttccatg
     3661 agcgcagcgg gctgccgttg ggcgcgagca cggcgagcag gtcgctgctg gcatcggcga
     3721 cgtagaggtt gccggcggcg tcggtggcga ttgcgcgcgg ctgcagcggg ctgccggacc
     3781 tggggaagtt gccgagctgg gcgagcagca caccgtcgct ggagtactgc tcgaccgtgt
     3841 tgctgaagtt cgggttggag aggtagacgt ggccgccggc gtcggtcgcg accgagagcg
     3901 ggaccggctg gaagccgatc gaggtgtggg ctcgctcgga ggcactcgcc accccgcccg
     3961 cgagggcgag aacggcgaga gccgtgagcc cggcgatcgt ggtccgaata gcgggagcca
     4021 cggaagcaga tggtagacgt aac
//
LOCUS       DASRIG010000260         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_905001, whole genome shotgun sequence.
ACCESSION   DASRIG010000260 DASRIG010000000
VERSION     DASRIG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905001"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="cysK"
                     /locus_tag="VFB52_07125"
     CDS             <1..753
                     /gene="cysK"
                     /locus_tag="VFB52_07125"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZO06143.1"
                     /translation="QGLIRPGRSVVVEPTSGNTGIALAMVCAARGYELILTLPEGMSR
                     ERAKLLRAYGAEVRETPSLGGMNEAVALAEEIREQRKAFMPMQFSNPANPEIHRRTTA
                     EEIWSACDGQIGAFVTGVGTGGTITGVGQLLKERSPETLVVAVEPASSPVLSGGVPGP
                     HKIQGIGAGFVPEVLDRAVIDEVIGVGDEDALETARLVAREEGVLAGISAGANINAAL
                     QVAARPELAGKRVVTIVCDSGERYMSLPFFSP"
     gene            750..1481
                     /gene="cysE"
                     /locus_tag="VFB52_07130"
     CDS             750..1481
                     /gene="cysE"
                     /locus_tag="VFB52_07130"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZO06144.1"
                     /translation="MSSWKRVVAEVRADVAAARQRDPAAQGVSSFEILTSWAGVQALL
                     AHRIAHALMEAGVPVLPRFIAYATRAVTGVEIHPAAQIGREFFIDHGAGVVIGETAVI
                     GNCVTLYQGVTLGGTGFQRGKRHPTLGDNVTVGSGAKLLGPIAVGDNAKVGANTVVVE
                     DVPPGATVVGNPGHPVRVEGKKVEGPDADWIHLPDPIAEAIKGLSGRLAEIERRLAEL
                     DGGNTPESSEPTEIRPRTGRSSAGG"
     gene            1564..2145
                     /locus_tag="VFB52_07135"
     CDS             1564..2145
                     /locus_tag="VFB52_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06145.1"
                     /translation="MLKYLLTVSAVALAALSLAACGGSDSDSDEDQITTAIETSVTTT
                     DPADCTVYSTQAFLEQSEGEKGEAAVTSCEEDVEDTSDDPDSVEVSAVEVDGDAATAE
                     VAFSGGGFDGQAVAVSLVKEDGDWKLDSIDGFAEFDRDAFVGSLEEGFATTELTDEQT
                     SCVNEAFDAATDEELEAVILEGEEALIEIVADC"
     gene            2167..2772
                     /locus_tag="VFB52_07140"
     CDS             2167..2772
                     /locus_tag="VFB52_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06146.1"
                     /translation="MRKRLSRLPLVCALVACAALVLAACGGGESDDEKIEATIVTAVK
                     GNDPASCTELLSQRVVEQLTKAEGREAIAKCEESAAEDTAVRSLRVADVRVADETATA
                     EVSFAGGTFDGQTVTMDLVEEDGQWKVDELTGFAGFDSARLLSTLKEIVFTPGSQSAG
                     EEAQAECILEELQAEPDAELEGLLLDPGSEVLENVFSVCVG"
     gene            2808..3386
                     /locus_tag="VFB52_07145"
     CDS             2808..3386
                     /locus_tag="VFB52_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06147.1"
                     /translation="MVAALVLSACGDGEEGDAAQIEATITASATSRDPADCRRYRTQR
                     MLEQATKVRGEVAIEACEETMLEDWERPTSVEVTRIEVDGDEATAQVATVGGVYSEQE
                     VLIALVEEDGTWMEDEFLGFAVFDREAFLLEFGREIMERARTRAHANANACVVGLMEQ
                     LSDAEIEAVLVDPSPQVFEDFVRPCEPRSARA"
     gene            3418..5688
                     /locus_tag="VFB52_07150"
     CDS             3418..5688
                     /locus_tag="VFB52_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZO06148.1"
                     /translation="MSLQGEFDVPEVPLEAAGGAPTEAGADRTERLLAGLNPPQREAV
                     EHTEGALLVLAGAGSGKTRVLTHRIAYLLATGAARPGEILAITFTNKAAAEMRERVGQ
                     LIGRSVRAMWVTTFHSSCARMLRADAEKLGYSKSFTIYDQADSVRMLKRAMAELGIDP
                     KRYPPRAIHSKISGAKNQLIDSDMYAETVGSLFEEAVAEAFPLYEKRMLEANAMDFDD
                     LLVRTVNALELYEEVRERWRRTFRHVLVDEYQDTNHAQYRLLQLLASEHGNLMVVGDE
                     DQAIYGFRGADIRNVLDFEDDFADASVIKLEQNYRSTQTILSAANAVVERNRERRPKR
                     LWSEEEGGDKVELAELGDEHEEARWVAGEIERLAEEEGLKRSDVAIFYRTNAMSRVLE
                     DTLNRYDVSYQVIGGTKFYERAEIKDAVAYLSLLVNPADLVSFQRIVNSPRRGIGNTS
                     QARLASYANTTGLTIWEVMERAEEVPGLGAAAIKAIGRFHETMDLLRGRVDAGEPVAE
                     VLQAVLSETGYLEALEAERTVEAEGRVENLEALREGAAEFDLEREQEGESETPPVEEY
                     LQQISLYSEQDGLEDAEELVTLMTVHNAKGLEYDTVFVVGCEDGSFPHMRALEEGGEE
                     EERRLCYVAITRARKRLYMTWARERRLFGRAERNLPSRFIDELPVELTERQASASTTL
                     GAMSWDQPAPNTDGEQRVDPGPALELQSGDDVIHASFGDGVVTAVEPGGVVVVRFAED
                     GTERKLMADYAPIRKR"
     gene            5691..6539
                     /gene="folD"
                     /locus_tag="VFB52_07155"
     CDS             5691..6539
                     /gene="folD"
                     /locus_tag="VFB52_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZO06149.1"
                     /translation="MAARVIDGKAVSAAVRERVRVDVAAYVEEAGRTPTLATVIVGED
                     PASQIYVANKHKACEQVGMNSVHHGLEAATSEEDLLALVRALGADDEVDGILVQLPVP
                     EHIDPDAVVAAIDPAKDVDGLTPTNAGLLAHGTPGLVPCTPAGVMELLAHEGVELEGA
                     EAVVVGRSKLVGVPVARLLLSANATVTICHSRTRDLDATCARADVLVAAVGVPRLLGA
                     AAVKPGAVVIDVGMNRLEDGLCGDVDYEAAAEVAAAITPVPGGVGPTTIAMLLVNTLQ
                     AARGRA"
     gene            6617..7189
                     /locus_tag="VFB52_07160"
     CDS             6617..7189
                     /locus_tag="VFB52_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06150.1"
                     /translation="MSERPGTGDVIAGVAGFILLLSLFVFSWFGIEDFGLDAFEALDD
                     WVGIILIFAAFSGLALALFGNDVPRADAVPLATVVLALGALSVLVILIQIIAPPSVTF
                     GPASADLDPEFGTFLGMVCAIALAIGGYLTLQEEDALGGSYGAPAAAPSAPVAPPAAQ
                     PPQTQVPPQQPAPPQQQAPPPPPPQPPAAG"
BASE COUNT         1201 a         2472 c         2629 g         1014 t
ORIGIN      
        1 caggggctga tccggccggg gcgctcggtt gtggtcgagc cgaccagcgg caacaccggg
       61 atcgcgctgg cgatggtctg cgcagcccgc ggctacgagc tgatcctcac cctgcccgag
      121 gggatgagcc gcgagcgggc gaagctgctg cgcgcctacg gggccgaggt gcgggagacg
      181 ccgtcgctgg gcgggatgaa cgaggcggtg gcgctggccg aggagatccg cgagcagcgc
      241 aaggcgttca tgccgatgca gttctccaac ccggcaaacc cggagatcca ccggcgcacg
      301 accgccgagg agatctggag cgcctgcgac ggccagatcg gtgccttcgt caccggggtc
      361 ggcaccggcg gcacgatcac cggggtcgga cagctgctga aggaacgttc gccggagacg
      421 ctggtcgtcg cggtcgagcc ggccagctcg ccggtgctct ccggcggcgt gcccggcccg
      481 cacaaaatcc aggggatcgg cgccggcttc gtgcccgagg tgctcgaccg cgcggtgatc
      541 gacgaggtga tcggggttgg cgacgaggac gcgctcgaga ccgcccgcct ggtcgcccgc
      601 gaggagggcg tactggcggg gatctcggcc ggcgccaaca tcaacgccgc gctgcaggtg
      661 gcggcccgcc cggagctcgc cggcaagcgc gtggtcacga tcgtctgcga ctcgggcgag
      721 cgctacatgt ccctaccctt tttctcacca tgagcagctg gaaacgagtg gtcgccgagg
      781 tccgcgccga tgtcgccgcg gcgcgccagc gcgatcctgc cgcgcagggg gtctccagct
      841 tcgagatcct gaccagctgg gccggggtgc aggcgctgct cgcccaccgg atcgcccacg
      901 cgctgatgga ggccggggtg ccggtgctgc cgcgcttcat cgcctacgcg acccgcgccg
      961 tgaccggggt cgagatccac ccggcggcgc agatcgggcg cgagttcttc atcgaccacg
     1021 gggccggtgt ggtgatcggc gagaccgcgg tgatcggcaa ctgcgtcacc ctctaccagg
     1081 gggtgaccct gggcgggacc gggttccagc gcggcaagcg ccacccgaca ctcggggaca
     1141 acgtcaccgt cggctccggc gccaagctgc tggggccgat cgcggttggc gacaacgcca
     1201 aggtcggcgc caacacggtg gtcgtcgagg acgtgccccc gggcgcgacg gtggttggca
     1261 acccgggcca cccggtgcgg gtcgaaggca agaaggtcga ggggcccgac gccgactgga
     1321 tccacctgcc ggacccgatc gccgaggcga tcaaggggct ctccggccgg ctcgcggaga
     1381 tcgagcggcg gctcgccgag ctcgacggtg gcaacacacc cgagagctcc gagccgaccg
     1441 agatccggcc gcgcaccggt cgttcgtccg ctggcggctg agcgctccga aggctcggtt
     1501 acgctggccg cgctcttgtc cgtccaccct ggcgtgttga tgcgcacgcc tgaaaggagc
     1561 cctgtgctca agtacctgtt gaccgtgtcc gccgtcgcgc tcgcggccct gtccctcgcc
     1621 gcctgcgggg gcagcgactc cgacagcgac gaagatcaga tcacgaccgc gatcgagacc
     1681 tcggtgacga cgaccgaccc ggccgactgc acggtctact cgacccaggc gttcctcgaa
     1741 cagtcggagg gcgagaaagg cgaggccgcg gtcaccagct gcgaagaaga cgtcgaggac
     1801 acctcggacg acccggactc ggtcgaggtc tccgcggttg aggtggacgg tgacgcggcc
     1861 accgccgagg tcgccttcag cggtggcggc ttcgacgggc aggcggtcgc ggtctcgctg
     1921 gtcaaggaag acggcgactg gaagctcgac agcatcgacg gcttcgccga attcgaccgc
     1981 gacgccttcg tcggcagcct cgaagaaggc ttcgcgacga ccgaactgac cgacgaacag
     2041 acgagttgcg tgaacgaagc gttcgacgcg gcgaccgacg aggaactgga agcggtgatc
     2101 ctcgagggcg aagaagcgtt gatcgaaatc gtcgccgact gctgatcggg cggagcggga
     2161 gtcgagttgc gcaagcgtct ctctcgactc ccgctcgtct gcgcgctcgt cgcgtgcgcc
     2221 gcgctcgtgc tcgccgcctg cgggggcggc gaaagtgacg acgaaaagat cgaagcgacg
     2281 atcgtcaccg cggtcaaagg caacgacccg gcctcctgca ccgagctgct gagccagcgg
     2341 gtcgtcgaac agctgaccaa agcggagggc agggaggcga tcgcgaaatg cgaggaaagc
     2401 gccgcggaag acaccgcggt gcgctccctg cgggttgccg acgtccgggt cgcggacgaa
     2461 accgccaccg ccgaggtcag cttcgccggc ggcaccttcg acgggcagac cgtcaccatg
     2521 gatctggtcg aggaagacgg gcaatggaaa gtcgacgagc tgaccggctt cgccgggttc
     2581 gactccgccc ggctgctctc gaccctcaaa gagatcgtct tcacgcctgg gagccagagc
     2641 gcaggagaag aagcccaagc cgaatgcatc ctcgaagaac tgcaagcgga gccggacgcc
     2701 gagctggaag ggctgttgct cgacccggga tcagaagtgc tcgaaaacgt gttctcggtt
     2761 tgcgtcggct aggaaacaag cgcggatcgt ggctggtcct ctgcggattg gtcgcggcac
     2821 tcgtcttgtc cgcctgcggt gacggggagg agggggacgc ggcccagatc gaagcgacga
     2881 tcaccgcctc ggcgaccagc agggatccag ccgactgccg ccgctaccgg actcagagga
     2941 tgcttgaaca ggccaccaag gtgcgtggcg aggtggcgat cgaggcttgc gaagaaacga
     3001 tgctcgaaga ctgggagcgg ccgacatcgg tcgaggtgac cagaatcgag gtcgacggcg
     3061 acgaagccac cgcccaggtg gccacggtcg gtggcgtcta cagcgagcag gaggtgctga
     3121 tcgcgctagt cgaggaagac ggcacctgga tggaagacga gttcctcgga ttcgccgtgt
     3181 tcgatcgcga ggcgttcctg ctcgagttcg gccgcgagat catggaacgg gctcgcacgc
     3241 gcgcgcacgc caacgccaac gcctgcgtgg tcggcctgat ggaacagctc agcgatgcgg
     3301 agatcgaggc ggtgctcgtc gacccctcgc cgcaggtgtt cgaagacttc gtccgcccct
     3361 gcgaaccccg gtccgcgagg gcctgaggcc cgccgcgcgc ctggctatgc tcgccgggtg
     3421 agcctgcaag gcgagttcga cgtccccgag gtgcccctgg aagccgctgg cggcgccccg
     3481 accgaggccg gtgccgaccg caccgagcgg ctgctcgccg gcctcaatcc gccccagcgg
     3541 gaggcggtcg aacacaccga gggggcgctg ctggtcctgg ccggggcggg ctccggcaag
     3601 acccgcgtgc tcacccaccg gatcgcctac ctgctcgcca ccggcgccgc ccggcccggc
     3661 gagatcctcg cgatcacctt caccaacaag gcggcggcgg agatgcgcga gcgggtcggc
     3721 cagctgatcg gccgctcggt gcgggcgatg tgggtgacga ccttccactc ttcctgcgcg
     3781 cggatgctgc gcgcggacgc cgaaaaactc ggctactcga agagcttcac gatctacgac
     3841 caggccgatt cggtccggat gctcaaacgt gccatggcgg agcttgggat cgacccgaaa
     3901 cgctacccgc cgcgcgcgat ccactcgaag atctccggcg ccaagaacca gctgatcgac
     3961 tccgacatgt acgcggaaac ggtcggcagc ctgttcgagg aagccgtggc ggaggccttc
     4021 ccgctctacg agaagcggat gctcgaagcc aacgcgatgg acttcgacga cctgctcgtc
     4081 cgcacggtca acgccctgga gctctacgag gaggtccgcg aacgctggcg gcgcaccttc
     4141 cgccacgtgc tcgtcgacga gtaccaggac accaaccacg cccagtaccg cctcctgcaa
     4201 cttttggctt cagagcacgg aaatttgatg gtagtgggcg acgaagacca ggcaatctat
     4261 ggcttccgtg gagccgatat caggaacgtt ctcgacttcg aggatgactt cgcagacgcc
     4321 tcggtgatca agctcgagca gaactaccgc tcgacgcaga cgatcctctc ggcggcgaac
     4381 gcggtggtcg agcgcaaccg ggagcggcgg ccgaagcggc tgtggagcga agaggagggc
     4441 ggcgacaaag tcgagctggc cgagctcggc gacgagcacg aggaggcgcg ctgggtcgcg
     4501 ggcgagatcg aacggctggc cgaggaggag gggctgaagc gctccgacgt cgcgatcttc
     4561 taccggacca acgcgatgag ccgagtgctc gaggacacgc tcaaccgcta cgacgtctcc
     4621 taccaggtga tcggcggcac caagttctac gagcgggccg agatcaagga cgcggtcgcc
     4681 tacctcagcc tgctggtcaa ccccgccgac ctcgtctcct tccagcggat cgtcaactcg
     4741 ccgcggcgcg ggatcggcaa caccagccag gcgcggctcg cgagctacgc caacaccacc
     4801 gggctgacga tctgggaggt gatggaacgg gcagaggagg tgccggggct gggcgccgcc
     4861 gcgatcaagg cgatcgggcg cttccacgag acgatggacc tgctgcgcgg ccgggtcgac
     4921 gcgggcgagc cggtggccga ggtgctgcag gcggtgctca gcgagaccgg ctacctggaa
     4981 gcgctggaag ccgaacgcac ggtcgaggcg gagggccggg tcgagaacct cgaggcgctg
     5041 cgcgagggcg ccgccgagtt cgacctcgag cgcgagcagg agggggagag cgagaccccg
     5101 ccggtcgagg agtacctcca gcagatctcc ctctacagcg agcaggacgg gctcgaggac
     5161 gccgaggagc tggtcacgct gatgaccgtg cacaacgcca aggggctgga gtacgacacg
     5221 gtcttcgtgg tcggctgcga ggacggctcg ttcccgcaca tgcgggcgct ggaggagggc
     5281 ggcgaagaag aggagcggcg gctctgttac gtcgcgatca cccgcgcccg caaacgcctc
     5341 tacatgacct gggcgcgcga acggcgcctg ttcgggcggg ccgagcgcaa cctgccctcg
     5401 cgcttcatcg acgagctgcc ggtcgagctg accgaacgcc aggcgagcgc ctcgaccacg
     5461 ctcggggcga tgagctggga ccagccggcg ccgaacaccg atggcgaaca acgggtcgat
     5521 ccggggccgg cgctggagct gcagtccggc gacgacgtca tccacgccag cttcggcgac
     5581 ggcgtggtca ccgcggtcga gcccggcgga gtggtcgtcg tccgcttcgc cgaggacggg
     5641 accgagcgca agctgatggc cgactacgcc ccgatcagga agcggtaacg atggcggccc
     5701 gggtgatcga cggcaaagcg gtctcggcgg cggtgcgcga gcgcgtccgc gtcgacgtcg
     5761 ccgcctacgt cgaggaggcg gggcggacgc cgaccctggc gacggtgatc gtcggcgagg
     5821 acccggcctc gcagatctac gtcgccaaca agcacaaggc ctgcgagcaa gtcgggatga
     5881 actcggtcca ccacgggctc gaggcggcga cctcggagga ggacctgctg gcgcttgtgc
     5941 gggcgctcgg tgccgacgac gaggtcgacg gcatcctcgt gcagctgccg gtccccgagc
     6001 acatcgaccc cgacgcggtc gtcgccgcga tcgacccggc caaggacgtc gacgggctga
     6061 ccccgaccaa cgccgggctg ctcgcccatg gcacgcccgg tctcgttccc tgtacgccgg
     6121 ccggggtgat ggagctgctc gcccacgagg gggtcgagct ggaaggggcg gaggcggtcg
     6181 tcgtcggccg ctcgaagctg gtcggcgtcc cggtcgcgcg cctgttgctc agcgccaacg
     6241 cgacggtgac gatctgccac tcgcgcaccc gggacctcga cgccacctgc gcccgcgccg
     6301 acgtcctcgt cgccgccgtc ggcgtgccgc gcctgctcgg cgccgccgcg gtcaagcccg
     6361 gcgcggtggt gatcgacgtc ggcatgaacc ggctcgagga cggcctttgc ggcgacgtcg
     6421 actacgaggc cgcggccgag gtcgccgccg cgatcacccc ggtgcccggc ggcgtcggcc
     6481 cgacgacgat cgcgatgctg ctcgtcaaca ccctgcaggc cgcccgcggc cgggcctagt
     6541 cgtccaaccc gcgaattgct gcccgaccgc gggtgtaggg tcgcggcatc gggttccacg
     6601 cagttaagga ggcaccatga gtgagcgtcc ggggaccggc gacgtgatcg ccggagttgc
     6661 tggtttcatc ctgctcctga gcctgtttgt cttctcctgg ttcgggatcg aagacttcgg
     6721 cctcgacgcc ttcgaagcgc tcgacgactg ggtcgggatc atcctcatct tcgccgcctt
     6781 ttccgggctg gcgctggcct tgttcggcaa cgacgtgccg cgcgccgatg ccgtgccgct
     6841 ggccacggtc gtgctcgcgc tcggcgcgct ctcggtgctg gtcatcctga tccagatcat
     6901 cgctccgccc agcgtcacct tcggcccggc gagcgccgac ctcgatcccg aattcgggac
     6961 cttcctcggc atggtctgcg cgatcgcgct ggcgatcggc ggctacctga cgctgcagga
     7021 agaggacgcg ctcggcggct cctatggcgc tccagcggcg gcgcccagcg ctcccgtcgc
     7081 tcccccggcg gcgcagcctc cgcagacgca ggttccgccc cagcagcccg caccgcccca
     7141 gcagcaggcg ccgcctccgc caccaccgca gccgccagca gcgggctgag gtggccgagg
     7201 gcaagcgcag ggggcgccgc cgtcggatcc ggctgcggcg tggcgaggcg atcgcggcga
     7261 tctccgcgat cgcgctgttc gtcctcatgt tcaccacctg gtacggctcg gaagtc
//
LOCUS       DASRIG010000261         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2995549, whole genome shotgun sequence.
ACCESSION   DASRIG010000261 DASRIG010000000
VERSION     DASRIG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995549"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFB52_07165"
     CDS             complement(<1..416)
                     /locus_tag="VFB52_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO06151.1"
                     /translation="MSEFYGSADEGEAIEVIHRALDLGVTFLDTADMYGPFTNEKLVG
                     KAIAGRREEVVVATKFGIVRGENGERLGIRGDAEYVRKSCEDSLQRLGVERIDLYYQH
                     RVDPEVSIEETVGAMADLVAAGKVRYLGLSEPGPQTV"
     gene            complement(522..1331)
                     /locus_tag="VFB52_07170"
     CDS             complement(522..1331)
                     /locus_tag="VFB52_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06152.1"
                     /translation="MAHRVLAIFTDGLHGEEPIEEIRRAGNDTGVDLRVICPAVEANA
                     FRHTLGDIDEPKVEAEERLQQVLAALRASGIEAAGEVGDPDPVQAAQDALLEAPADEV
                     LLFEHEEAQARWFEGGLFERAQEGLEPPLRMIALHSDGAGTPDHVVEVEEAGPGLYNP
                     DEGHEIGGSYLPGLSRGDFAGMVFGIFGTIIVAILAAAVAAKGGQESGPEAAAILIAI
                     GVALVNLAHVVGLTLMESVRYRGGFATFFRLLALIGTPLAILANLLLLVLA"
     gene            complement(1360..1665)
                     /locus_tag="VFB52_07175"
     CDS             complement(1360..1665)
                     /locus_tag="VFB52_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06153.1"
                     /translation="MSFGVEENEEEELAVEEAEAAAEEAGAIGGKVQHDAEDPAEQPL
                     AEAGEGEAEGFELAERDLEDIASHGDEHRFPDNVPSDPEEPSEAEYGEADEAIPADE"
     gene            complement(1703..1894)
                     /locus_tag="VFB52_07180"
     CDS             complement(1703..1894)
                     /locus_tag="VFB52_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06154.1"
                     /translation="MSIKQKIARKAVKKTAKHTTRGAASKLKRTPMRSTTLLGLGAAF
                     GLLAGWMLGRSGGEPEPAA"
     gene            1946..2620
                     /locus_tag="VFB52_07185"
     CDS             1946..2620
                     /locus_tag="VFB52_07185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO06155.1"
                     /translation="MAPIAILDIDGTLVDTNYHHAIAWHRALRGHGHPIALWRIHRHI
                     GMGGDQIVAALAGDEVEERDGDSIREAEGAAYAELIGEVEPMAEARELIEDLREGGWR
                     VVLASSAKAEEVDHYLNLLDARDLVDAWTTAADVEQTKPAPDLVQTALAKVGGEGRAL
                     MVGDSAWDVKAASAAEVPTLAVLTGGFSAAELRDAGAAEVVESIAELRSAGAGDSPLQ
                     LFLAHR"
     gene            complement(2570..2908)
                     /locus_tag="VFB52_07190"
     CDS             complement(2570..2908)
                     /locus_tag="VFB52_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06156.1"
                     /translation="MPPRGVKKGSKRDRQYKKIKKAQKKEGRSEKRAEEIAARTVNKE
                     KARSGESKTKSKSSTRGRSASSRGGKQSGKGPAGRTKEQLYNDAKKLGIEGRSTMSKK
                     QLQRAVAGAS"
     gene            complement(2908..3450)
                     /locus_tag="VFB52_07195"
     CDS             complement(2908..3450)
                     /locus_tag="VFB52_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZO06157.1"
                     /translation="MNLQKLEGKKVAFLMANEGVEQVELTEPLEAVRGSGAEVELLAL
                     EAGEVQAFNHLDKAQTFPVDRVVGEADASEYDGLVLPGGVANPDQLRTKEGALEFVRA
                     FFESGKPVGAICHAPWTLIEAGVLGDRTVTSWPSLQTDLRNAGATWVDEEVHVDNGLV
                     TSRKPDDLPAFTSTIIEELV"
BASE COUNT          514 a         1331 c         1277 g          655 t
ORIGIN      
        1 acggtctgcg gcccgggctc cgagagcccc aggtagcgga ccttgcccgc cgccaccaga
       61 tcggccatgg cgcccaccgt ctcctcgatg gagacctccg ggtcgacccg gtgctggtag
      121 tagaggtcga tccgctcgac cccgagccgc tgcagcgagt cctcgcagga cttgcgcacg
      181 tactcggcgt cgccgcggat accgagccgc tcgccgttct cgccgcgcac gatcccgaac
      241 ttcgtcgcca ccacgacctc ctcgcggcgc ccggcgatcg ccttgccgac cagcttctcg
      301 ttcgtgaacg gcccgtacat gtcggcggtg tcgaggaagg tgacaccgag gtcgagcgcg
      361 cggtggatga cttcgatggc ctcgccttcg tcggcgctgc cgtagaactc ggacatgccc
      421 atgcagccga gaccaagagc cgatacctcg gttcccgagg aaccgagctt ccgcttttcc
      481 atattcagcc tttctgtgtg gggggatctg gtccgcgcgg gctaggcgag aaccagcaac
      541 aagaggttcg caaggatcgc cagcggggtc ccgatcaggg ccaggaggcg gaagaaggtg
      601 gcgaagccgc cgcggtagcg gacgctctcc atcagggtga ggccgacgac gtgcgccagg
      661 ttgaccaggg cgacgccgat cgcgatcagg attgccgcgg cctcggggcc gctctcctgg
      721 ccgcccttgg cggcaaccgc ggcggcgagg atcgcgacga tgatcgtccc gaagatcccg
      781 aagaccatgc cggcgaagtc accgcgcgag aggccgggca ggtaggagcc gccgatttcg
      841 tggccctcgt cggggttgta gaggccgggc ccggcctcct cgacctcgac cacgtggtcg
      901 ggggtaccgg cgccgtcgct gtgcagggcg atcatccgca gcggcggctc cagcccttcc
      961 tgggcgcgct cgaagagacc accctcgaac cagcgcgcct gcgcctcctc gtgctcgaag
     1021 agcaggactt cgtcggccgg ggcctccagc agggcgtcct gcgcggcctg gaccgggtcg
     1081 ggatcgccga cctcgccagc cgcctcgatg ccgctcgcgc gcagcgccgc cagtacctgc
     1141 tggaggcgct cctcggcctc gactttcggc tcgtcgatgt cgccgagcgt gtgacggaag
     1201 gcgttcgcct cgaccgccgg acagatcacc cgcaggtcca cccccgtgtc gttaccggcg
     1261 cgcctgatct cctcgatcgg ctcctctccg tgcaacccgt cggtgaagat cgccagaacc
     1321 cggtgcgcca tctctctacc ccctcagttc atcgtgtggt cactcgtcgg ccgggatcgc
     1381 ctcgtcggcc tccccgtact cggcctcgct cggctcctcc ggatcggagg ggacgttgtc
     1441 ggggaagcgg tgttcatcgc cgtgcgaggc gatgtcttcc aggtcccgct cggccagctc
     1501 gaagccctcg gcctcgccct cgccggcctc ggccagcggc tgctccgcgg ggtcctcggc
     1561 gtcgtgctga actttgcctc cgatcgctcc ggcttcctcg gcggcggcct cggcctcttc
     1621 gaccgcgagc tcttcttcct cgttctcttc cactccaaag ctcatgccca ttgagtaccc
     1681 ctgcctggaa cggagaaaca ggctaggccg ccggctccgg ctcgccgccc gagcggccca
     1741 gcatccaacc ggccagaagg ccgaaggcgg ctccgaggcc gagcagggtg gtcgagcgca
     1801 tcggcgtccg cttcagcttc gacgcggccc cgcgggtggt gtgcttcgcg gtcttcttga
     1861 ccgccttgcg ggcgatcttc tgtttgattg acatggagct cggctacccg tttgcgtccg
     1921 gcgacacacc gggtagccgc cggccgtggc accgatcgcg atcctcgaca tcgacggcac
     1981 cctggtcgac accaactacc accacgcgat cgcctggcac cgggcgctgc gcggccacgg
     2041 gcacccgatc gcgctctggc ggatccaccg acacatcggc atgggcggcg accagatcgt
     2101 cgccgcgctg gccggggacg aggtcgagga gcgcgacggc gactcgatcc gcgaggccga
     2161 gggcgctgcc tacgccgagc tgatcggcga agtcgagccg atggcggaag cgcgcgagtt
     2221 gatcgaggac ctgcgtgagg ggggctggcg ggtcgtgctc gccagctcgg cgaaggccga
     2281 ggaggtcgac cactacctca acctgctcga cgcccgcgac ctggtcgacg cctggaccac
     2341 cgccgccgac gtcgagcaga ccaagccggc ccccgacctg gtgcagaccg cgctggcgaa
     2401 ggtcggcggc gagggcaggg ccctgatggt cggcgactcg gcctgggacg tgaaggcggc
     2461 gagcgcggcc gaggtgccga cgctggccgt gctcaccggc gggttctcgg cggcggagct
     2521 gcgcgatgca ggcgccgccg aggtggtcga gtcgatcgcc gagctgcgct cagctggcgc
     2581 cggcgacagc ccgctgcagc tgtttcttgc tcatcgttga gcggccttcg atgccgagct
     2641 tcttggcgtc gttgtagagc tgttccttgg ttcttccggc ggggcctttg ccggactgtt
     2701 tgccaccgcg actgctggcg gagcgcccac gagtcgagga tttcgatttc gttttcgact
     2761 cgcctgagcg cgccttttcc ttgttcacgg tgcgggcggc gatctcctcg gcgcgtttct
     2821 ccgagcgccc ctctttcttc tgcgcttttt tgattttttt gtactggcgg tcgcgtttgc
     2881 ttcctttttt cactccgcgt ggtggcatca gacgagctcc tcgatgatcg ttgacgtgaa
     2941 cgcaggcagg tcgtcgggtt tgcggctggt gacgaggccg ttgtcgacgt ggacctcctc
     3001 gtcgacccac gtggccccgg cgttgcgcag atcggtctgc aggctcggcc aggaggtgac
     3061 cgtgcggtcg ccgaggacgc cggcctcgat cagcgtccag ggcgcgtggc agatcgcccc
     3121 gaccggcttg ccggactcga agaaggcgcg cacgaactcc agcgccccct ctttggtccg
     3181 cagctgatcc gggttggcga ccccgcccgg cagcaccagc ccgtcgtatt cgctcgcgtc
     3241 ggcctcgccg acgacgcggt cgaccgggaa tgtctgcgcc ttgtcgaggt ggttgaacgc
     3301 ctggacctcg ccggcctcca gggccagcag ctcgacctcg gcgccgctgc cgcgcaccgc
     3361 ttccaggggc tcggtcagct cgacctgctc gaccccctcg ttggccatca ggaaggcgac
     3421 tttcttccct tccagcttct gcaggttcat gccgttcgct cctttcctgc ctgatcggca
     3481 tcttcaacct atgggtgccg gacccggtcg ccgcacccac acaagcgggt tggtcttctc
     3541 acccgagcgg gaaccgggac cagccggtaa aagccgctgg gaaccgggcc ggcacgctcg
     3601 ccctgcgatt acccctgcac gtgcggcgca aacgaagccg gcgggtcagc cgaccaggtc
     3661 gagcacggcg cgctcggtct tggccagtga gcgcgagggc tcgatcgtgg cgccgtcgcg
     3721 gaagcagtcg aagacgcgcg ggtcgatgta ggagcggcga cagaccgcgg gggtgtt
//
LOCUS       DASRIG010000262         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_130004, whole genome shotgun sequence.
ACCESSION   DASRIG010000262 DASRIG010000000
VERSION     DASRIG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130004"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFB52_07200"
     CDS             <1..726
                     /locus_tag="VFB52_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZO06158.1"
                     /translation="YADMTRRYMAASGASAEDFARVVVKNQHNGGLNPRAQYGGELTV
                     EQVLESREVVSPLTLLMCSPISDGAAAVVLISEAAAKRRGARGPRIRASVVRSGNRHD
                     RDDPTAGSTGLAAAAAFEQAGVGPEDLSCAEVHDASAPAELMVYEQLGLAPPGGGPEL
                     IESGRTKLDGALPVNTSGGLLSKGHPIGATGIAQICESTWQLRGDAGPRQVDGAKLAL
                     TQNGGGWLEGDSAAMSVHILSAD"
     gene            complement(790..1530)
                     /locus_tag="VFB52_07205"
     CDS             complement(790..1530)
                     /locus_tag="VFB52_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06159.1"
                     /translation="MTENGGTAAGPVISVRDLSTGYGGNPVVHDLSFDVKAGEVLCLL
                     GPNGAGKTTTMLALAGELPLLTGEVEFAGVGGKAPLFKRARNGLSYVTEERSVFKAMT
                     LRDNLRVGGVDPQEVIELFPELEQRMGTRGGLLSGGEQQMLTLGRALARKPRLLLADE
                     LSLGLAPLVVDRLLQAVRRSADEDGTAVIMVEQHAHKALRYTDHAIVIRRGRVGLDLT
                     GEDARKRISEVEHAYLTSVDGNGASTPS"
     gene            complement(1530..>2543)
                     /locus_tag="VFB52_07210"
     CDS             complement(1530..>2543)
                     /locus_tag="VFB52_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06160.1"
                     /translation="KYIPLIGGVSLIVLLLVNQNGIVKEQIAQLAFVRSKLAGAMPFL
                     AIKEKPKRELPKPSADHEMKVSPKALEVQNLTVKYGGTVAVDDVSLTVRPGQVLGLIG
                     PNGAGKTSLIDAVTGFTKAQPEAVLRLDGHDISRWSATKRARAGMGRSFQSLELFEDS
                     TVLENLRAASDPRDFSSYVRDLVWPTDVPLPDRVLSTINEFELEDDLDATVEDLSYGR
                     RRLLAMARAVATESSVLLLDEPAAGLGDAETAELAHLVGRLAKEWGIAVLLVEHDMNF
                     VMSVCDELVVLDFGRKIAEGPPSAVRNDPAVIAAYLGTEESDEEVAKQGSLPASITGQ
                     EGV"
BASE COUNT          362 a          896 c          911 g          374 t
ORIGIN      
        1 tacgccgaca tgacgcgccg ctacatggcc gcgtcggggg cgagcgccga ggacttcgcc
       61 cgggtggtgg tcaagaacca gcacaacggc ggcctcaacc cgcgcgccca gtacggcggc
      121 gagctgactg tcgagcaggt actggagagc cgcgaggtgg tctcccccct gaccctgctg
      181 atgtgctcgc cgatctccga cggcgccgcc gcggtggtgc tgatcagcga ggcggcggcg
      241 aagcgccgcg gcgcccgcgg cccgcgcatc cgcgcctcgg tcgtgcgctc cggcaaccgc
      301 cacgaccgcg acgacccgac cgccggctcg accggcctgg ccgcggccgc ggccttcgag
      361 caggccggcg tcggccccga ggacctgagc tgcgccgagg tccacgacgc cagcgccccc
      421 gccgagctga tggtctacga gcagctcggc ctcgcgcccc ccggcggcgg ccccgagctg
      481 atcgagtccg gccgcaccaa gctcgacggc gccctgcccg tcaacaccag cggcggcctg
      541 ctctccaagg gccacccgat cggcgccacc ggcatcgccc agatctgcga gtccacctgg
      601 cagctacgcg gcgacgcggg cccgcggcag gtcgacggag cgaagctggc gctgacgcaa
      661 aacggcggcg gctggctcga aggcgactcc gccgcaatga gcgtccacat cctctcggcc
      721 gactagccga ttccgcgagt ctgcaattga ccccggtata cggggccaaa tgcagactca
      781 ggaggagctt taggaagggg tgctggcgcc gttgccgtca acgctggtca ggtaggcgtg
      841 ctcgacctcg gagatccgct tgcgggcgtc ttcgccggtc aggtcgaggc cgacgcggcc
      901 gcggcggatg acgatcgcgt ggtcggtgta gcggagcgcc ttgtgggcgt gctgctcgac
      961 catgatcacg gcggtgccgt cctcatcggc ggagcggcgg accgcctgca ggaggcggtc
     1021 gacgaccagc ggggcgaggc cgagcgagag ctcgtcggcc agcagtaggc ggggcttgcg
     1081 ggcgagggcg cggccgaggg tgagcatctg ctgctcgccg ccggagagca ggccgccgcg
     1141 ggtgcccatc cgttgctcca gctcggggaa gagttcgatc acctcctgtg ggtcgacgcc
     1201 gccgacccgc aggttgtcgc gcagggtcat ggccttgaac acggagcgct cctcggtcac
     1261 gtaggagagg ccgttgcggg cgcgcttgaa cagcggtgcc ttgccgccga ccccggcgaa
     1321 ctcgacctcg ccggtgagca gcggcagttc gccggccagg gccagcatcg tcgtcgtctt
     1381 gcccgccccg ttggggccga gcaggcagag gacttcgccg gccttgacgt cgaagctgag
     1441 gtcgtggacg accgggttgc cgccgtagcc ggtcgagagg tcgcggaccg agatcaccgg
     1501 ccccgccgcg gttccgccgt tctccgtcat cacacaccct cctgaccggt gatgctcgcc
     1561 ggcagcgagc cctgtttggc gacctcctcg tcgctctcct cggtaccgag gtaagcggcg
     1621 atcaccgccg ggtcgttgcg gaccgccgag ggcgggccct cggcgatctt gcggccgaag
     1681 tcgagcacga ccagctcgtc gcagaccgac atcacgaagt tcatgtcgtg ctcgaccagg
     1741 agcacggcga tcccccactc tttggccagg cggccgacca ggtgggcgag ctcggcggtc
     1801 tcggcgtcgc cgaggccggc ggcgggctcg tcgagcagca gcacgctcga ctcggtcgcg
     1861 acggcgcgcg ccatcgccag caggcgccgg cgcccgtagg agaggtcctc gaccgtcgcg
     1921 tcgaggtcgt cctcgagctc gaactcgttg atcgtgctca gcacccggtc gggcagcggc
     1981 acgtcggtcg gccagaccag gtcgcgcacg taggagctga agtcacgcgg gtcgcttgcc
     2041 gcgcgcaggt tttccagcac ggtcgagtcc tcgaacagct ccagcgactg gaacgagcgg
     2101 cccatcccgg cgcgggcccg tttggtcgcc gaccagcggc tgatgtcgtg cccgtcgagc
     2161 ctcaggaccg cctccggctg ggctttggtg aagccggtga cggcgtcgat cagcgaggtc
     2221 ttgccggcgc cgttggggcc gatcaggccc agcacctggc ccggcctgac agtcagcgaa
     2281 acgtcgtcga cggcgaccgt gccgccgtat ttgacggtca ggttctggac ctcgagcgcc
     2341 ttcgggctga ccttcatctc gtggtcggcg ctcggcttcg gcagctcgcg cttcggcttc
     2401 tccttgatcg ccaggaaggg catcgccccg gccagcttcg agcgaacgaa ggcgagctgg
     2461 gcgatctgct ccttgacgat cccgttctgg ttgaccagca gcaggacgat cagggagacg
     2521 ccgccgatca gcgggatgta ctt
//
LOCUS       DASRIG010000263         5156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2458522, whole genome shotgun sequence.
ACCESSION   DASRIG010000263 DASRIG010000000
VERSION     DASRIG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458522"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFB52_07215"
     CDS             complement(<1..947)
                     /locus_tag="VFB52_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO06161.1"
                     /translation="MSQVPFVLYAARAFPLLKQGYSLIRKWNALPDTERAAVQDQGRR
                     AVAAIIAVKTAATAARSAEDPPSSWEEALEAVRNPDPAAEMAVAIVEYLQGVPEATRG
                     TIAAAVGAHGKDDANFERALAGAQRDGYIHRSGVDFRGIKWDTTEWADRQVLDTPRVR
                     ELEERIVGFLTDFGLASLDHISGRLGLEDDAPELRAALERAIVDESLHWYGNGIYGLP
                     LGRLLDFRPKLDIWAETKPPASEKDLGDAIDELDTAIRSLAAAMKATGIHRRTPAGEV
                     HPTDESGDEPYEDLRRLGELRDSGVLTEGEYAAKKSELLA"
     gene            complement(1005..1775)
                     /locus_tag="VFB52_07220"
     CDS             complement(1005..1775)
                     /locus_tag="VFB52_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06162.1"
                     /translation="MKVPKQVTAGTKSAKRGESGTLRVWFALIAPATFGAIVYAAAED
                     SWSILALSAIVSACAFSGGAFLGFLFGIPVYFAKAGSGDGADKPSYQPNTNLTQVSDW
                     LTKIIIGVGLVQFTQLAGTIGRLGDSLSSGFGGEPTGKPYGIALVVGYALIGFLTSYL
                     YTRLRLQWAFAQADRGAFEEILEDRDKADDRALSLTDQQLDPTKPQPLPDELAAAIAQ
                     ASPVAKGQILAKAQEKSAAAGNRADAIVGAIEERHAEE"
     gene            1922..2323
                     /locus_tag="VFB52_07225"
     CDS             1922..2323
                     /locus_tag="VFB52_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06163.1"
                     /translation="MSSSLVALAVGLALTAAVVVVGLRRNLSETTREGLPEFGGDPEG
                     TTPKLDLYEGQERPRGPLSRRQQIWLSTIYLLLALLQLVDVVRSADDRLLHLGFALFF
                     VLGFVLVVLRKHPAEAGFFRSGSRKSGAEPN"
     gene            2382..3218
                     /locus_tag="VFB52_07230"
     CDS             2382..3218
                     /locus_tag="VFB52_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06164.1"
                     /translation="MNSFRKLRALVPAAVVALLAVALLAPSANAANIPPFTSTAQYKA
                     LAQFVEKLNGLSNTPATAAQKASYEDQLENKHGAAVNKSTALFNRAKKVAKAEATRAF
                     KKGSKTIRNTEAGELAALRREYDAQMNSAAAKHERDLGRIEDEFDRRTDSLKKQVKRL
                     RKQKAEAESAVRKEQIQEAIERRTERVEGDRKLLQEEITDLKSGYRREKEAIRAAKAS
                     ATKSVQQNDDEAIETLRNRNNRIYNTKVRTLQNRRANQLGNLEAKDNAGRAAIARMPA
                     AS"
     gene            complement(3265..3753)
                     /locus_tag="VFB52_07235"
     CDS             complement(3265..3753)
                     /locus_tag="VFB52_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06165.1"
                     /translation="MAPTKTKKASGSKAKPAAKKAAAKRKRTTPKRATPRRKASSAKP
                     AATSNGAAHKPSANGHSSKAAIPLLAGGAVLAGTIGGVLIGASNSGKKVLGVSLPQPK
                     RMQVKLKSSDLASAAKQVGRFGENVGELTAELKRAREGLVGDGKHDSPIEVLLRGLSA
                     RR"
     gene            4009..4923
                     /locus_tag="VFB52_07240"
     CDS             4009..4923
                     /locus_tag="VFB52_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZO06166.1"
                     /translation="MNVLIAADMEGVAGIEGYGDCLPSHPAAYAAGRRLMTDEVLVAV
                     EALRAGGAGRISVGDWHMVGTNVERERMPAGVEVRPIAELALHEAEPSMAKAHGGPLD
                     AVVFLGHHASTRNPRGFSSHTFVWEMEVLLDGEPLNEVQVYAQALAAEGVPVLAASGD
                     RWMLEELGATELGGATLVPTKEGRGRARADSRPPAEVHAELSAAIAAALASPPEPPRA
                     RAYPAELVIRAEGKELAATTVATPEDLLTAIATTFRTSQVSREYRQLAKLLPAAEGSR
                     LRGLHRRAGSVLATPVMRAKERRWLAAT"
     gene            complement(4940..>5156)
                     /locus_tag="VFB52_07245"
     CDS             complement(4940..>5156)
                     /locus_tag="VFB52_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06167.1"
                     /translation="TTEVAALKHETDPEKALAWLVECEQEAIEALQAAIEPTGREGRS
                     EALEHMLEHQIMRKQEQADFLARALAA"
BASE COUNT          790 a         1752 c         1759 g          855 t
ORIGIN      
        1 gccagcagct cggacttctt ggcggcgtac tcgccctcgg tcagcacccc cgagtcgcgc
       61 agctcgccca gccgtcgcag gtcctcgtag ggttcgtcgc cgctctcgtc cgtcgggtgc
      121 acctcgcccg cgggggtgcg gcgatggatg ccggtcgcct tcatcgcggc ggcgaggctt
      181 ctgatcgccg tgtcgagctc gtcgatcgcg tcgcccaggt ccttttcgct ggccggcggc
      241 ttcgtttccg cccagatgtc cagtttcggc cggaagtcga ggaggcggcc cagcggcagg
      301 ccgtagatcc cgtttccgta ccagtggagg ctttcgtcga cgatcgcccg ctccagcgcc
      361 gcccgcagct cgggtgcgtc gtcttcgagc ccgagacgcc cggagatgtg gtccaggctg
      421 gcgaggccga agtcagtcag gaagccgacg atccgctcct ccagctctct gacccggggc
      481 gtgtccagca cctgcctgtc ggcccattcg gtggtatccc acttgatccc acggaagtcg
      541 accccgctgc ggtggatata gccgtccctc tgagcgccgg cgagcgcgcg ctcgaagttc
      601 gcgtcgtcct tcccgtgtgc gccgaccgcg gccgcgatcg tgccgcgggt ggcctcgggc
      661 accccctgga ggtactcgac gatcgcgacc gccatctcgg ctgccgggtc cgggttgcgg
      721 accgcctcga gcgcctcctc ccagctggaa ggggggtcct cggcgctgcg cgccgccgtc
      781 gccgcggtct tcacggcgat gatcgccgcg accgcgcggc ggccctgatc ctggacggcc
      841 gcacgttcgg tatccggcag cgcgttccat ttcctgatca gcgagtaccc ctgtttgagc
      901 aaggggaagg cgcgggccgc gtagagcacg aatggcacct gactcaccgc tcccgcagtg
      961 tcaggtaaca aaaatgttgc cgggacgtga caccggcggc gggctcactc ttccgcatgt
     1021 cgttcctcga tcgcgccgac gattgcgtcg gcgcgattgc ccgccgccgc gcttttctcc
     1081 tgtgctttgg cgaggatctg gcctttcgcc accggtgaag cctgcgcgat cgccgccgca
     1141 agttcgtccg ggagcggctg cggtttggtc gggtcgagct gctggtcggt cagcgacaag
     1201 gcgcggtcgt ccgccttgtc gcggtcttcg aggatttcct cgaaggcgcc gcggtcggcc
     1261 tgggcgaatg cccactgcag gcgcaggcgc gtgtagaggt agctggtcag gaagccgatc
     1321 agcgcgtagc cgacgaccag ggcgattccg tacggttttc ccgtcggttc accgccgaac
     1381 cccgaggaga gtgagtcgcc cagcctgccg atcgtcccgg ccagctgggt gaactggacg
     1441 agtccgaccc cgatgatgat cttggtcagc cagtcggaga cctgggtcag gttggtgttc
     1501 ggttgatagc tcggtttgtc ggcaccgtcc ccgctgcccg ctttggcgaa gtacaccggg
     1561 atcccgaaca ggaagccgag gaaggcgccg ccggagaagg cacaggcgga gacgatcgcc
     1621 gagagggcga ggatcgacca cgaatcctcc gccgccgcgt agacgatcgc accgaaggtg
     1681 gccggtgcga tcagcgcgaa ccacacacgc agggtccccg actccccccg tttcgctgac
     1741 ttggtccccg ccgttacctg tttcggtact ttcaccgcgg aggagacttc cacagtaatc
     1801 cgagtgtgtc aacacaccct gactaattag gcgacgtgaa caagtgagtt cgcactcccg
     1861 cggtgccggc gtcgctcttc ctcccgccgg gtctttcgtc tgctcgcggt atcttgagta
     1921 gatgagcagc agccttgtgg cgctggcggt tggacttgcg cttaccgccg cggtcgtcgt
     1981 cgtcgggtta cggcgcaatc tctccgagac cacgcgggag gggctgccgg agtttggggg
     2041 agaccctgag gggacgaccc cgaaactgga cctgtacgag ggccaggaaa ggccacgagg
     2101 cccgctctcc cggcggcagc agatttggct ctcgacgatc tacctgctgc ttgcgctctt
     2161 gcagctggtc gatgtggttc ggtcggccga cgatcggctg cttcatctgg gctttgccct
     2221 gttctttgtg ctggggttcg tgctggtcgt gctgagaaag caccccgcgg aagcggggtt
     2281 tttccgttca gggtcgagga aatcgggcgc ggagccgaat taagggggag aaccccaaag
     2341 cgcccggctc gggcgcccgt ccaaggagtg tcccgattcc gatgaactcg ttccgaaagc
     2401 tgcgtgcgct cgtccccgcg gcggtggtcg cactgcttgc ggtcgcactg ctcgcgccca
     2461 gcgccaatgc ggcaaacatc ccgcccttca cctcgaccgc gcagtacaag gccctggcgc
     2521 agttcgtcga gaagctcaac gggctctcga acacgccggc aaccgccgcc cagaaagcca
     2581 gctacgagga tcagctggag aacaagcacg gggccgcggt gaacaaatcg accgcgctct
     2641 tcaaccgcgc caaaaaggtg gcaaaagccg aagcgacgcg cgccttcaaa aagggctcga
     2701 agacgatccg caacaccgag gccggggaac tcgcggccct gcgccgtgaa tacgacgcgc
     2761 agatgaactc ggccgcggcc aagcacgaga gggatctggg ccggatcgaa gacgaattcg
     2821 accgccgcac cgactcgctg aaaaaacagg tcaaacgcct gcgcaaacag aaggccgaag
     2881 ccgaaagcgc cgtccgcaaa gaacagatcc aggaagcgat cgaacggcgg accgaacggg
     2941 tcgaaggcga ccgcaagctc ctccaggaag aaatcaccga cctgaagtcc ggctaccgcc
     3001 gcgaaaagga agcgatccgc gccgccaagg cgtcggcgac caaatcggtg cagcagaacg
     3061 acgacgaagc gatcgaaacg ctgcgcaacc gcaacaaccg gatctacaac accaaggtgc
     3121 gcacgctgca gaaccgtcgc gccaaccagc tgggcaacct cgaagccaag gacaacgccg
     3181 gtcgcgccgc gatcgcgcgc atgccggccg ccagctagtc agcagctact cgccgggggc
     3241 cccgtaacgg ggctcccggc ttctttaccg ccgtgccgaa aggccgcgga gcagcacctc
     3301 gatcggggag tcgtgcttgc cgtcgccgac caggccctcg cgggctcgtt tcagctccgc
     3361 ggtcagctcg ccgacgttct cgccgaagcg gccgacctgc ttcgccgcgc tggcgaggtc
     3421 gctcgacttc agcttgacct gcatccgctt cggttgcggc aggctcacac cgaggacctt
     3481 cttgcccgag ttgctggcgc cgatcagcac gccgccgatc gtgccggcaa gcacagcgcc
     3541 accggccagc agggggatcg ccgccttgct ggagtggccg ttcgcggacg gtttgtgagc
     3601 ggctccgttg ctcgtcgcgg ctggtttagc cgagctcgct ttccgcctcg gagtcgcccg
     3661 tttcggggtc gtccgtttcc gtttcgcggc agcttttttc gcggcgggtt tggctttcga
     3721 gccgcttgct tttttcgttt tggtcggtgc catctgaatc tctcctcgcg tcggtggccg
     3781 gactcccgcc tcccgcccgg gggcacttca tcgcgggtac ccctcgttag ggaagttgaa
     3841 ccaatcggcg ggatgccccg atcagcggac caaccggtac ttgagggtct ttgagcccgg
     3901 gtcgtagccg acgctgcgaa cggcggtgga ggtgactcgg gtgcgcctca caccagcatt
     3961 gtccagatag cggacgcttg cggaatccgg gcgggatatg ttgggaacgt gaacgtcttg
     4021 atcgccgccg atatggaggg cgtcgcgggc atcgagggat acggcgactg cctgccctcg
     4081 caccccgccg cctacgcggc gggccggcgg ctgatgaccg acgaagtgct ggtcgcggtc
     4141 gaggcgctgc gggccggtgg ggcggggcgg atctcggtcg gggactggca catggtcggt
     4201 accaacgtcg aacgtgagcg gatgccggcg ggggtcgagg tgcggccgat cgccgagctg
     4261 gctctgcacg aggccgagcc ctcgatggcg aaggcgcacg gcggcccgct cgacgcggtc
     4321 gtcttcctcg gtcaccacgc cagcacgcgc aacccgcgcg ggttctcctc gcacaccttc
     4381 gtctgggaga tggaggtgct gctcgacggc gagccgctga acgaggtcca ggtctacgcg
     4441 caggcgctgg ccgccgaggg cgtgccggtg ctcgcagcga gcggtgaccg ctggatgctc
     4501 gaggagctcg gggcgaccga gctgggcggg gccacgctcg tcccgaccaa ggaggggagg
     4561 ggccgcgctc gcgcggactc gcgcccgccg gccgaggtcc acgccgagtt gtccgctgcg
     4621 atcgccgccg cgctggcgtc cccgccggag ccgccgcgcg cccgcgccta cccggccgag
     4681 ctggtgatcc gcgccgaggg caaggagctg gcggcgacca cggtcgcaac gcccgaggac
     4741 ctgctgacgg cgatcgcgac caccttccgc actagccagg tctcgcgcga gtaccgccag
     4801 ctggcgaagc tgctgccggc cgcggaagga tcgcgcctgc gcggtttgca ccggcgcgcg
     4861 ggcagcgtcc tggcgacgcc ggtgatgcgc gccaaggagc ggcgctggct cgccgccacc
     4921 tgaccgcgac cggcgccggc taggccgcga gcgcccgcgc caggaagtcg gcctgttcct
     4981 gcttgcgcat gatctggtgc tcgagcatgt gctcgagggc ctcggaacgg ccctcgcggc
     5041 cggtcggctc gatcgcggcc tgcagggcct cgatcgcttc ctgctcgcac tcgaccagcc
     5101 acgccagcgc cttctccggg tccgtctcgt gcttcagcgc ggcgacctcg gtggtc
//
LOCUS       DASRIG010000264         4127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1695638, whole genome shotgun sequence.
ACCESSION   DASRIG010000264 DASRIG010000000
VERSION     DASRIG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695638"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="VFB52_07250"
     CDS             <1..1837
                     /locus_tag="VFB52_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06168.1"
                     /translation="ADGRYLAFRGVIGGFDGIFLKDLTTGDTLRVVGVSAYDPNVSQL
                     RATAPSISADGRYVSFTTQARLDPANDLAASSADVYVADLAGPVPSYELASARDGCDP
                     ATASPCGLTYAGVGSGSDASARVALSADGRKVVFVTTAESDLAGPGTPRLQVAVRDLD
                     ADTTTLVSVERDPETGAMTGKPVAGGALLVANAEPFLLRGAAISADGTTVAWLGTNLA
                     AQVPLLPAEAKRIAEAPRPYDEPLWRRVAAGSSAPTRRIVAGDGAADPFPDMTTKNTD
                     MNGFQGWLGVTKGINGVPQLSADGRIVALIGNPTEAANAFLVDMTPGLSRAQALRQIT
                     RETVVDPADPVGTVNALGRISLTGHVFDVALSGSGRQVAFATARQRFPLAPPNLITAP
                     PASMGMVELYFVDLQTEAMQRLTHGLGGQDEPSFSALPNPETSAQEGLGATAPTLGGN
                     RIAFASTASNLVAEDSNEASDVFLLADQLESGPPGGSVVPPGPAPWQKKTPWKLTLSA
                     FSLPDGGVRIVAGVPAAGKLRTKVAAEPGSKPLAAKQLVQARASAKRGGRVAIELELP
                     RRLRGLARTREGVYATASVSFHHAGRKVLRGRVQIRFHVHAKRAK"
     gene            1834..3510
                     /locus_tag="VFB52_07255"
     CDS             1834..3510
                     /locus_tag="VFB52_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06169.1"
                     /translation="MRRVALSMLLALLAVGALAGPAGAAETWRSQQPPGANGSYPLLG
                     EVGDVECWAANRCVLIAGGNGGVAAGIFAYDGDRWYRYSTVCGGEQGRVAWAGPTEFW
                     TVSTQQAGQATGEEPPPAISLCHFKDGQVVASYGEPVGVASSYMQMYGAACNGPSDCW
                     FGGARLPGTPNVGAFHLHWDGATVTPTPSLTTVSELSDPGRRVADIAYHDGSFYEAVD
                     VEEGDQPNPEEEVARDEFGLGPSLVHRLSPGTSTPFKPLIGAEPLDLGEGSPTDLGGT
                     LLAGAEGEDLWAVAGSTGEPTPVVLRLGKSGPAQLALSDPGAVLAQSVEVRAAAADPG
                     ADRVWVALRRFGDGNETLLSPARLTSVAADGTVAPEVTLPLPGDESGGEPVGNKGVAS
                     ALACPGPEQCWLATRKGWLFHLGPNPAPNADPVMHTLITSRPADNSLPSLPPLELPQD
                     DSGLEEGGNEQGPEIGEEPLPPKVIPPLVRKIKFRMVTDTLLEMSFTLREKARVQLLG
                     YRAGAKVAKTPKRVLAKGRRSLRLELDPKAWPTRFDLEAKATKKKKKGSK"
     gene            3507..>4127
                     /locus_tag="VFB52_07260"
     CDS             3507..>4127
                     /locus_tag="VFB52_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06170.1"
                     /translation="MSPRVRARRRAVLAVFAALVATVTIVLAVPGAGAENGELVVQPS
                     LGAPTSSFLGATPQEAAGEVWATSNSSLTLARYTDAGGWALLPAPTAKPGSTLTLAFG
                     ASAGRTTPRGGVIVAAREEDEFGGIPDVLLVRDPGGNLRQTADATPLLESGEGLFGFA
                     NEASGSGRLLTATDEAGGRTGAFVVPQLRNRVLAYDGEEWSAEEICL"
BASE COUNT          625 a         1527 c         1425 g          550 t
ORIGIN      
        1 cgccgacggc cgctacctcg ccttccgggg cgtgatcggt ggcttcgacg gcattttcct
       61 caaggacttg acgaccggag acacgcttcg ggttgttggt gtcagcgcct acgacccgaa
      121 cgtgagccag ctgcgggcga ccgcgccttc gatctccgcc gacggccgct atgtcagctt
      181 caccacccag gcgcggctgg accccgccaa tgatctggcg gcgagctccg ccgacgtcta
      241 tgtcgccgat ctcgccgggc cggtcccgag ctacgagctc gcctcggcgc gcgacggctg
      301 cgacccggcg accgcgagcc cctgcggcct cacctacgcc ggtgtcggct cgggctccga
      361 cgccagcgcc cgggtagcgc tcagcgccga cgggcgcaag gtcgtcttcg tcaccaccgc
      421 cgagtcggac ctcgccgggc cgggaacgcc gcgcctgcag gttgccgtgc gcgacctcga
      481 cgccgatacg accaccctgg tcagcgtcga acgcgacccc gaaaccgggg cgatgacggg
      541 taaaccggtg gccggcgggg cgctgctggt ggccaatgcc gagccgttcc tgttgcgggg
      601 tgccgcaatc agcgccgacg gcaccacggt tgcctggctg gggacgaatc tggccgcgca
      661 ggtgccgctc ctcccggccg aggcgaaacg gatcgccgaa gcgccgcgcc cctacgacga
      721 gccgctctgg cgtcgcgtcg ccgccgggtc gagcgcgccg acccggcgga tcgtcgccgg
      781 cgacggtgcc gccgacccgt tccccgacat gaccacgaaa aacacggaca tgaatggttt
      841 ccagggatgg ctcggagtga cgaaagggat caacggggtg ccgcaactga gcgcggacgg
      901 gcgcatcgtc gccttgatcg gcaacccgac cgaagccgcc aacgcctttc tcgtcgacat
      961 gactcctggg ctgagccgag cgcaggccct gcgacagatc actcgcgaga cggtcgtcga
     1021 ccccgccgac ccggtcggca cggtgaacgc gctgggacgt atctcgctca ccgggcacgt
     1081 tttcgacgtc gcgctttcag gtagcggccg ccaggtcgcc ttcgccaccg cacgccagcg
     1141 cttccccctg gcgccaccga atctgatcac cgcgcccccg gcgagcatgg gcatggtcga
     1201 gctctacttc gtcgatctcc agaccgaggc gatgcagcgc ctcacccatg gcctcggcgg
     1261 gcaggacgaa ccttcgttca gcgcgctccc gaatcccgaa acgtccgccc aggaaggcct
     1321 cggcgcaacc gctccgaccc tcggcggcaa ccgcatcgcc ttcgcctcca ccgcctccaa
     1381 cctggtcgcc gaagacagca acgaagcaag cgacgtcttc ctgctcgcag accagctcga
     1441 atcgggtcct cccggcggct cggtggtccc gcccggcccg gcgccctggc agaagaagac
     1501 gccctggaag ttgaccctga gcgccttctc gctgcccgac ggcggggtca ggatcgtcgc
     1561 cggggtgccg gccgccggca agctgcggac gaaggtcgcc gccgaacccg gcagtaaacc
     1621 gctggcggca aagcagctgg tccaggcgcg ggcgagcgcc aagcgcggcg gccgcgtcgc
     1681 gatcgagctc gagttgcccc gccgcctgcg cggcctcgcc cgcacccgcg aaggcgtcta
     1741 cgcgacggcg agcgtcagct tccaccacgc gggccgcaag gtgctgcggg gcagggtcca
     1801 gatccgcttc cacgtgcacg cgaagagggc caagtgaggc gcgtcgccct ctcgatgctc
     1861 ctggcgctgc tcgcggtcgg cgcccttgcc ggccccgccg gcgcggccga gacctggcgc
     1921 tcccagcagc cccccggtgc caacggcagc tacccgctgc tcggggaagt gggggacgtg
     1981 gagtgctggg cggcgaaccg ctgcgtgctg atcgccggcg gcaacggcgg cgtcgccgcc
     2041 gggatcttcg cctacgacgg cgaccgctgg taccgctact caaccgtctg cggcggcgag
     2101 caagggcgcg ttgcctgggc cggcccgacc gagttctgga ccgtctccac ccagcaggcc
     2161 gggcaggcga ccggcgaaga gccgccgccg gcgatctcgc tctgccactt caaagacggc
     2221 caggttgtcg cttcctacgg tgagccggtc ggcgtcgcca gctcctacat gcagatgtac
     2281 ggggcagcct gcaacgggcc cagcgactgc tggttcggcg gcgcccgcct ccccgggacc
     2341 cccaacgtcg gtgccttcca tctgcactgg gacggcgcga cggtcacccc gaccccctcg
     2401 ctgacgactg tgtccgaact ctccgatccg ggtcgtcgcg ttgccgacat cgcgtaccac
     2461 gacggcagct tctacgaggc cgtcgacgtc gaggaaggcg accagccgaa ccccgaagaa
     2521 gaggtcgcgc gggacgaatt cggtctcggc ccctcgctcg tgcaccggct ctccccgggc
     2581 acctcgaccc cgttcaaacc gctgatcggg gccgaaccgc tggacctcgg cgaaggctcg
     2641 cccactgacc tcgggggcac cctgctggcc ggcgccgagg gcgaagacct gtgggcggtc
     2701 gccggctcga ccggcgaacc gacgccggtc gtgctgcggc tcggcaaatc cggcccggcc
     2761 cagctggcgc tgagcgatcc gggtgcggtg ctggcccaga gcgtagaagt gagagccgcc
     2821 gcagccgatc ccggggcgga ccgggtctgg gtcgccctgc ggcgtttcgg cgacgggaac
     2881 gaaacgctcc tgtctccggc ccggctgacc tctgtggcgg ccgacggcac ggtcgcaccc
     2941 gaggtgacgc tgccgctgcc cggcgacgaa agcggtggtg agccggtcgg caacaaaggc
     3001 gtcgcctccg cgctcgcctg ccccgggccc gaacagtgct ggctggcgac ccgcaaaggc
     3061 tggctcttcc acctcggccc caacccggcg ccgaacgccg acccggtgat gcacacgctg
     3121 atcacctcgc gaccggctga caacagcctg ccgagcctgc caccgctgga acttccccag
     3181 gacgactccg ggctggaaga agggggcaac gaacagggcc ccgagatcgg cgaagagccg
     3241 ctgccgccga aggtgatccc gccgctggtc cgcaagatca agttccggat ggtcaccgac
     3301 acgctgctcg agatgagttt cacgctgcgt gagaaggcgc gggtgcagct actcggctac
     3361 cgcgccggcg ccaaggtggc gaagacgccg aagcgggtgc tggcgaaggg ccggcgcagc
     3421 ctgcgcctcg agctcgatcc caaggcctgg ccgacgcgct tcgacctcga ggccaaagcg
     3481 acgaagaaaa agaagaaggg ctcgaagtga gcccccgggt ccgggcgcgg cgacgcgccg
     3541 tgctggcggt tttcgctgcc ctggtcgcga ccgtgacgat cgtgctcgcg gtccccgggg
     3601 cgggggcgga gaacggcgaa ctggtcgtcc agccctcgct cggagcgccg accagcagct
     3661 tcctcggtgc gacgccgcag gaggccgcgg gggaggtctg ggcgacgtcg aacagcagcc
     3721 tcacgctagc ccggtacacc gacgccggcg gctgggcgct gttgccggcg ccgaccgcca
     3781 aaccgggttc cacgctgacg ctcgccttcg gcgcctccgc cgggcgcacg actccccgcg
     3841 gcggtgtgat cgtcgccgcc cgcgaagaag acgaattcgg cgggataccc gacgttctcc
     3901 tggtccggga cccggggggg aacctgcgcc agaccgccga cgcgacgccg ctgctcgaat
     3961 ccggcgaggg cctgttcggc ttcgccaacg aagccagcgg cagcggccgg ctgctgaccg
     4021 cgaccgacga ggccggcggc aggaccggcg ccttcgtcgt cccgcagttg cgcaaccgcg
     4081 tgctcgccta cgacggcgaa gaatggtcgg ctgaggaaat ctgcctg
//
LOCUS       DASRIG010000265         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1445629, whole genome shotgun sequence.
ACCESSION   DASRIG010000265 DASRIG010000000
VERSION     DASRIG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445629"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFB52_07265"
     CDS             complement(<1..805)
                     /locus_tag="VFB52_07265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZO06171.1"
                     /translation="MPAPGVPWAGRLYTVPAMAAADSEPQTDVSMEEIVALCKRRGFV
                     FPSSEIYGGLGSTYDYGHYGVLLKNNVKAEWWRAMLQERDDIVALDSAILQHPRVWEA
                     SGHLEGFTDPLVQCLGKCKKRWREDHLREAVEAEGGDPNAELVCPECGGELSEPRQFN
                     LMFETHVGPVAGEGSEVYLRPETAQGIFINFKNVLQFARRKPPFGIAQIGKSFRNEIT
                     PGNFIFRTREFEQMEMEFFCKPGTDDEWHQHWLQARFDWFKRLGVREENL"
     gene            839..1153
                     /locus_tag="VFB52_07270"
     CDS             839..1153
                     /locus_tag="VFB52_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZO06172.1"
                     /translation="MPIYEYKCENGHVFDVIQKMSDEALTECQECGAPAVRVLHPVAV
                     HFKGSGFYNTDYGKKKKGAAATSSESSSDSKSSDSGSGDSGSSSGSGDSKSESKAAAS
                     AD"
     gene            complement(1155..3086)
                     /locus_tag="VFB52_07275"
     CDS             complement(1155..3086)
                     /locus_tag="VFB52_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZO06173.1"
                     /translation="MSLRRGVGLVAVLILVAAVARLGGSEPAAAAWLPAVAATPDGTE
                     TGVSGAQVATDAAGNVTAVWTVGAVGSRAVRSAFRPAGGDWQAAVNRIGLTATFDCHD
                     PRLAVDANGDAAVVAECEKPAAAIRAAYRPGASWSSGAEISGSGSGATPRVGIADSGE
                     AIVVWAGPGATVQSSSHTLAGSAWSAAVQVSAGGETAEAPNVGVNAATGYAHAAWLKP
                     RKETVSDPVVEVRGARRAPAGTWAAPFNITQPAFSTTPVVTEEPQIYVSTSGERLYGY
                     QLLSTTLSLSSRRSFSDFAGYSEPPGSVTDPPGGVEAPRFALGGTNVGTAVWRTFPEG
                     GGVLVRAAVTSGNSWTGVTTIGGPAGTSIGTEPVLAADAAGDATIAWYAGGGIESARR
                     LAAGGFEPKQTISNAANPGFGSPAVAMTDAGDGLAVWSAEADHLAFAVDDRTPPTLTP
                     SVAASAQAGAPATMSAAVSDLWSDVSVSWDFGDGSSARGLAVSHAYATAGPKTVTVTA
                     TDAAGNTTSATRSVAVSPAPGSGGGGAGARKVTLKLKIPKQSRAAIQRAGAVKVKCKL
                     DTAGKCAGKVKLNGKAAGRGSVKAIAKRFAVLKLKLKPKTLKAIAEATAPVRLKLQLS
                     ATAPGRLAAKATRTLTIRP"
     gene            complement(3142..4407)
                     /locus_tag="VFB52_07280"
     CDS             complement(3142..4407)
                     /locus_tag="VFB52_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZO06174.1"
                     /translation="MPDDGEKRPSGPPDYKVYRSRKGFLSRLRSPDLSRLRGGSKPPG
                     GRPPREPGDGGERGPGRERRERRKRGGGSGVNGRKIAKWVGIAVLGWILLSFIAFGVS
                     AQIQSFKLSGEAKEALHGNPLLLPSAQTILVLGTDARPPGVNDGAEASEECFEQQSKG
                     EKPHDPCFEGEFRADTLMLIRAGGGAFRKLSIPRDTFAEIPGHEAGKINGAFAFGGAA
                     LQIKTIEQFLGIEIDHIAIVDFTGFEKLIDAVGGVEVDVPVKLCADISGGAGGGQGGQ
                     TIRLHKGTNTLNGEKALAYSRVRKPSECPGTGASAFTLGYDDLDRAKAQQAVINGIKD
                     RLTDPLRIPYNFVKGPIIGWEAPKAFVSDMGFFTMPQLVLAAGIGGTGDNEVLCGDLA
                     ESGCGFGPGGSIEVPEEVRRQAVKRLIDG"
     gene            4493..5452
                     /locus_tag="VFB52_07285"
     CDS             4493..5452
                     /locus_tag="VFB52_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO06175.1"
                     /translation="MSEAKTIGVDLGGTKMLVGALSGTEVLHESREASTGQTEDALVE
                     LLVREVNEAHEACPDAVAVGLGIPATINQQTGIAVGAVNLPIADLPIRELISERVRLP
                     VFIDNDANVAALAEFVYGAAQGKPNVVMLTIGTGIGGGLILGGEVYRGATGAGAELGH
                     TVIQADGPPCQGNCPNHGCVEALASGTALGREGKAAAESAPDSAIGKLLAAGETVDGT
                     AVTEAAIAGDETSVAVFDLIGSRLGVACASFANIFEPDAIVIGGGVIAAGDLLLDPVR
                     REIAERALRPMNETPVLAATLGNDAGMIGAAALARTELERGGG"
BASE COUNT          770 a         1911 c         2033 g          838 t
ORIGIN      
        1 gcaggttttc ttccctgacg ccaaggcgtt tgaaccagtc gaagcgcgct tgaagccagt
       61 gctgatgcca ttcgtcgtcg gtgccgggct tgcagaagaa ctccatctcc atctgctcga
      121 actcgcgcgt gcggaagatg aagttgcccg gcgtgatctc gttgcggaag gacttgccga
      181 tctgggcgat cccgaagggc ggcttgcggc gcgcgaactg gagcacgttc ttgaagttga
      241 tgaagatgcc ctgcgccgtc tcgggccgca ggtagacctc cgagccctcg ccggccaccg
      301 gcccgacgtg ggtctcgaac atgaggttga actggcgcgg ctcggagagc tcgccaccgc
      361 actcggggca gaccagctcg gcgttggggt cgccgccctc ggcctccacc gcctcgcgca
      421 ggtggtcctc gcgccagcgc ttcttgcact tgccgaggca ttggacgagc gggtcggtga
      481 agccctcgag gtggccagag gcctcccaga cgcgggggtg ctggaggatc gccgagtcga
      541 gggcgacgat gtcgtcgcgc tcctgcagca tcgcccgcca ccactcggcc ttgacgttgt
      601 tcttgagcag caccccgtag tggccgtagt cgtaggtcga gccgaggccg ccgtagatct
      661 ccgaggaggg gaagacgaag ccgcgccgct tgcagagcgc gacgatctcc tccatcgaga
      721 cgtcggtctg cggctctgag tcggcggcgg ccatcgccgg aaccgtatag agacggccgg
      781 cccagggcac gcctggcgcc ggcatcgcgg cagccggctg cgataatcgc cgaccgcgat
      841 gccgatctat gagtacaaat gcgagaacgg gcacgtcttc gacgtgatcc agaagatgag
      901 cgacgaggcg ctcaccgagt gccaggagtg cggcgccccc gcggtgcgcg tcctgcaccc
      961 ggtcgccgtc cacttcaagg gctccggctt ctacaacacc gactacggca agaagaagaa
     1021 gggcgcggcc gcaacctcca gcgagagctc ctcggactcg aagtcctcgg actccggctc
     1081 gggcgactcc ggttccagct ccggctccgg cgactccaag agcgagtcga aggccgccgc
     1141 cagcgccgac tgagctacgg gcggatcgtc agcgtccggg ttgccttggc ggcgaggcgg
     1201 ccgggtgcgg tggcgctgag ctgcagcttg aggcggaccg gcgcggtcgc ctccgcgatc
     1261 gccttcagcg tcttcggctt gagcttcagc ttcagcacgg cgaagcgctt cgcgatcgct
     1321 ttgaccgatc cgcggccggc ggctttgccg ttcagcttca ccttgccggc gcacttgccg
     1381 gcggtgtcga gcttgcactt gaccttcacc gctccggccc gctgaattgc cgcccggctc
     1441 tgtttcggga tcttcagctt cagcgtgacc ttgcgcgcgc cggctccgcc gccgccactg
     1501 ccgggggcgg gggagaccgc gaccgaccga gtcgcgctgg tcgtgttgcc ggcggcgtcg
     1561 gtggcggtga ccgtgaccgt cttcggcccg gccgtcgcgt aggcgtggga gaccgcgagg
     1621 cccctggcgc tgctcccgtc gccgaagtcc cagctgacgg agacgtcgga ccagaggtcg
     1681 ctcacggccg ccgacatcgt cgcgggcgct cccgcctgcg cgctcgccgc gacgctcggc
     1741 gtcagcgtcg gcggcgtgcg gtcgtcgaca gcgaaggcga ggtggtcggc ttcggccgac
     1801 cagaccgcga gcccgtcgcc ggcgtcggtc atcgccaccg cggggctgcc gaagcccggg
     1861 ttcgccgcgt tggagatcgt ctgcttcggc tcgaagccgc cggctgccag gcgccgggcc
     1921 gactcgatcc ccccgcccgc gtaccaggcg atcgtcgcgt cgccggcggc gtcggcggcg
     1981 agcaccggtt cggtcccgat gctggtgccg gccggcccgc cgatcgtcgt cacgccggtc
     2041 cagctgttgc cggaggtgac cgcggcgcgg acgagcacgc cgccaccttc ggggaaggtg
     2101 cgccagaccg cggttccgac gttggttccg ccgagcgcga agcgcggcgc ttcgacgccg
     2161 cccggcgggt cggtaacgga gccgggcggt tcggagtagc cggcgaagtc gctgaaggag
     2221 cggcgtgagc tgagactcag ggtcgtcgac agcagctggt agccgtagag ccgttcgccg
     2281 ctcgtgctga cgtagatctg cggttcttcg gtgaccaccg gcgtggtcga gaaggccggc
     2341 tgggtgatgt tgaagggggc ggcccaggtg ccggccggcg cgcgccgtgc tccccgcacc
     2401 tcgaccaccg ggtccgagac ggtttctttg cgcggcttca gccaggccgc gtgggcgtag
     2461 ccggttgcgg cgttgacgcc gacgttcggc gcttccgcgg tttcgccgcc ggcgctgacc
     2521 tggaccgccg cgctccaggc gctgccggcg agggtgtggg aggagctctg cacggtcgcc
     2581 ccgggcccgg cccagacgac gatcgcttcg ccgctgtcgg cgatcccgac ccgtggggtc
     2641 gcccccgagc ccgacccgga gatttcggcg ccactgctcc agctggcgcc ggggcgatag
     2701 gcggcgcgga tcgcggccgc gggtttctcg cactcggcga ccaccgccgc gtcgccgttc
     2761 gcgtcgaccg cgaggcgcgg gtcgtggcag tcgaaggtcg cggtcagacc gatccggttg
     2821 accgccgcct gccagtcgcc gcccgcgggt cggaaggcgg agcggacggc ccgcgagccg
     2881 accgcgccga cggtccagac cgcggtcacg ttgccggccg cgtcggtcgc gacctgggcc
     2941 ccgctgacgc ccgtttcggt tccgtccgga gtggccgcga ccgccggcag ccaggccgcc
     3001 gcggcgggct cggagccgcc gagccgggcg accgcggcga cgaggatcag caccgcgacc
     3061 aaaccgaccc cccgacgcaa gctcatcgac cgagcttagg ggaggtctcc gccgccgccg
     3121 cgaagctgga cctttctacg gctaaccgtc gatcagccgc ttgaccgcct ggcggcggac
     3181 ttcctcgggc acttcgatcg agccgccggg gccgaagccg cagccggact cggcgaggtc
     3241 gccgcagagg acttcgttgt cgccggtgcc gccgatcccg gcggcgagca cgagctgggg
     3301 catggtgaag aagcccatgt cggagacgaa cgctttcggc gcctcccagc cgatgatcgg
     3361 gcccttgacg aagttgtagg ggatccgcag ggggtcggtg aggcggtcct tgatcccgtt
     3421 gatcacagcc tgctgggcct tggcgcggtc gaggtcgtcg tagccgaggg tgaaggcgct
     3481 ggcgcccgtg cccgggcatt cgctcggctt gcgcacgcgc gagtaggcga gggccttttc
     3541 cccgttcagg gtgttggtgc ctttgtgcag gcggatcgtc tggccgccct ggccgccgcc
     3601 ggcgccgccg gagatgtcgg cgcagagctt caccggcacg tcgacctcga cgccgccgac
     3661 cgcgtcgatc agcttttcga agccggtgaa gtcgacgatc gcgatgtggt cgatctcgat
     3721 cccgaggaac tgttcgatcg tcttgatctg cagggcggcg ccgccgaagg cgaaggcgcc
     3781 gttgatcttg cccgcttcgt ggccggggat ctcggcgaag gtgtcgcgcg ggatcgagag
     3841 tttgcggaag gcaccgccgc cggcccggat cagcatcagg gtgtcggcgc ggaattcgcc
     3901 ttcgaagcag gggtcgtgcg gcttctcgcc cttcgactgc tgttcgaagc actcctcgga
     3961 agcttcggcg ccgtcgttga cgccgggcgg tcgcgcgtcg gttccgagca ccaggatcgt
     4021 ctgcgcgctc ggcagcagca gggggttgcc gtgcagagcc tccttcgctt cgccggagag
     4081 cttgaagctc tggatctggg cggagacgcc gaaggcgatg aagctcagca agatccatcc
     4141 caggacggcg atcccgaccc atttggcgat cttcctgccg ttgaccccgg agccgccgcc
     4201 gcgcttgcgc cgttcgcgcc gttcgcggcc aggcccgcgc tcgccgccgt cgccgggctc
     4261 gcgcggaggg cgccccccag gcggcttgga accgccccgc aagcgggata ggtcgggcga
     4321 cctcagtcgc gacaggaagc ctttgcggga ccggtagacc ttgtagtcgg gcggccccga
     4381 cggccgtttc tcgccatcgt cgggcatcga ggacgaaaca acatagacgg tcgaagttcc
     4441 gcaaaaagct gcgaagcgtc gtaagccgcg aaggacggct agcctagcgc cggtgagtga
     4501 ggcgaagacg atcggggtcg acctgggggg cacgaagatg ctcgtcgggg ccctctcggg
     4561 taccgaagtc ctgcacgaga gccgcgaggc ctcgaccggc cagacggagg acgcgctggt
     4621 cgagctgctg gtccgcgagg tcaacgaggc gcacgaagcc tgccccgacg cggtcgcggt
     4681 cgggctcggg atcccggcga cgatcaacca gcagaccggg atcgcggtcg gcgcggtcaa
     4741 cctgccgatc gccgacctgc cgatccgcga gctgatcagc gaacgggtcc ggctgccggt
     4801 cttcatcgac aacgacgcca acgtcgcggc gctggccgag ttcgtctacg gggccgccca
     4861 gggcaagccg aacgtagtga tgctgacgat cgggacgggg atcggaggcg ggctgatcct
     4921 cggcggcgag gtctaccgcg gcgccaccgg cgccggtgcc gagctcggcc acacggtgat
     4981 ccaggcggac ggcccaccct gccagggcaa ctgcccgaac cacggctgcg tcgaggcgct
     5041 cgcctccggg accgcgctcg ggcgcgaggg caaagcggcg gccgaatcgg cgccggactc
     5101 ggcgatcggc aagctgctgg cggcggggga gacggtcgac ggcacggcgg tgaccgaagc
     5161 ggcgatcgcc ggcgacgaga cctcggtcgc ggtcttcgac ctgatcggca gccggctcgg
     5221 ggtcgcctgc gccagcttcg ccaacatctt cgagcccgac gcgatcgtga tcggcggcgg
     5281 cgtgatcgcc gccggcgacc tgctgctcga cccggtccgg cgcgagatcg ccgaacgggc
     5341 gctgcggccg atgaacgaga ccccggtgct ggcggcgacg ctcggcaacg acgccgggat
     5401 gatcggcgcc gcggcgctgg cccgaaccga gctggagcgg ggtggcggct gatgcccggc
     5461 cgcctgatcg tctgtcccac tccgatcggc aacctcgacg acgtcagccc gcggatgcgc
     5521 gaggcgctgg tcggcgccga ctacatcgcc tg
//
LOCUS       DASRIG010000266         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3251895, whole genome shotgun sequence.
ACCESSION   DASRIG010000266 DASRIG010000000
VERSION     DASRIG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251895"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VFB52_07290"
     CDS             complement(<1..1998)
                     /locus_tag="VFB52_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZO06176.1"
                     /translation="MLAVALALLGLAAFAPAAFGNAELRVNTLQDRVDGNCGPNTSPI
                     FGCSLREALELTAEGENVLNEHGQPLISGEILVTFDVTPGTIEIGDRGTLDLTPGPGI
                     TGLKIVGPGNANLTVNAEKKDRAFEVHGGNVTITGLTIAKGFEDDFAINGSGGGALYQ
                     SGGDVTLEGLRLTENGVESGRDGGAIDVEGGDLTVVGSVLEKNSTEASLSEASGGAIN
                     VDASGSTVTLKETEVTNNVAGEGEGGGIYQDEGNLVIEDSVISDNKAANGGGGIAAIP
                     DGSHTTTIVGSTISNNSANDDRGGGILADTGSGGLNLIESTVSGNKASEGGGIAVEGP
                     ATVTASTISGNEASTSDSGGGVLVATDTLVLDTTTLAQNKGGALFADDGETQIRASTI
                     SANTNSNGEVGGIYGREEEATIKVRSSIVAGNTGMNGGSADCAGEITTEGHNIVGTIE
                     SNCEWSQGEGDQLGATGFQLGTLADNGGPTETMAPISATSIVINHGGNPKPQDQRNLP
                     RPVPTGAENTDVGAVEVQAPVNKVAPVAEAPEGMLEGESIVCKPGTWDNDTITSGVTY
                     SYAWFADNSPIGTESTYTLKAADANKDIACRVAANNGVTTSTEVESNSLKLDPGALSL
                     TPSSHDFGNRKAGSGPSAPQSFTLKNEGGTAITVTGATLTPE"
     gene            2168..2641
                     /locus_tag="VFB52_07295"
     CDS             2168..2641
                     /locus_tag="VFB52_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO06177.1"
                     /translation="MNLGPSKNLTDSAAQLRMAIVRTARRLRQEAAAEASGLTPTSTS
                     ALAAIDVHGPLTPSELAVIERVKRPTMTRTLGCLEREGLIERTPDPADGRSSLIAINA
                     AGRERLRRLRGRKNAYLARRMRDLSVEEVDTLERAAEILDRMREGEPRSGRESAS"
     gene            2638..3885
                     /locus_tag="VFB52_07300"
     CDS             2638..3885
                     /locus_tag="VFB52_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06178.1"
                     /translation="MTYHLRRSFSSLEVPNYRRYFVGQLVSLSGTWMQNVAAIWVILT
                     LTDNGFAVGLATALQFLPMLLFGAWGGLLADRFSKRRLLLTTQVLMMVPAVGLLVVTA
                     TGVVTPWMVYLGVFGFGLLNAIDNPTRQSFVIEMVGADRVVNAVSLNSVIVQAARIVG
                     PAIAGILIATAGVVPCFAINALTSVAMILALLRMDTRRLHEPPTATREAGAIRAGLRY
                     VRRTPELAVPLALMALVGTFGFNFQVVLPLLAKFSFDGGAATYGALVAAMGVGSIAGA
                     LITGHQGKTGPRLIAASALAFGLSAVGAAAMPALALEIPLLVLLGGAAVTFAATINSS
                     LQLAVAPEMRGRVMALYSVVFLGSTPIGAPLVGWLSEAYDPRVALLLAGIAGLSAAWA
                     AHVSFARLRERAGTDSRPLTAAN"
BASE COUNT          495 a         1326 c         1368 g          754 t
ORIGIN      
        1 ttcgggggtg agcgtcgcgc cggtcacggt gatcgcggtg ccgccttcgt ttttgagcgt
       61 gaacgactgt ggggcgctcg gcccggagcc ggctttgcgg ttgccgaagt cgtgcgagct
      121 cggggtcagc gagagcgccc cggggtccag cttcagcgag ttcgattcga cctcggtcga
      181 ggtcgtcacg ccgttgttcg ctgcgacccg gcaggcgatg tccttgttgg cgtcggccgc
      241 tttcagcgtg taggtcgatt cggtgccgat cggggagttg tcggcgaacc aggcgtagga
      301 gtaggtgacg ccgctggtga tcgtgtcgtt gtcccaggtg cccggtttgc agacgatcga
      361 ctcgccttcg agcatgccct cgggcgcttc ggccaccggc gcgactttgt tgaccggggc
      421 ctggacctcg accgcgccga cgtcggtgtt ttcggcgccg gtcggcaccg gccgcggcaa
      481 gttgcgctgg tcctggggtt tcgggttgcc gccgtggttg atcacgatgc tggtggcgct
      541 gatcggcgcc atcgtctcgg tcgggccgcc gttgtcggcg agcgtcccga gctggaagcc
      601 ggtggcgccg agctggtcgc cttcgccctg cgaccactcg cagttcgact cgatcgtgcc
      661 gacgatgttg tggccttcgg tggtgatttc accggcgcag tcggcggagc cgccgttcat
      721 cccggtgttg ccggcgacga tcgagctgcg gaccttgatc gtcgcctctt cttcgcggcc
      781 gtagatcccg ccgacctcgc cgttggagtt ggtgttggcg gagatcgtcg aggcgcggat
      841 ctgggtttcg ccgtcgtcgg cgaacagggc cccgcctttg ttctgagcca gcgtcgtcgt
      901 gtcgaggacg agcgtgtcgg tggcgacgag cacgccgccg ccggagtcgg aggtgctggc
      961 ttcgttgccg ctgatcgtgc tcgcggtgac cgtcgccggg ccttcgaccg cgattccgcc
     1021 gccttcggag gctttgttgc cggagaccgt cgattcgatc aggttgagcc cgccggaacc
     1081 ggtgtcggcg aggatgccgc cgccgcgatc gtcgttggcg ctgttgttgg agatcgtgga
     1141 gccgacgatc gtggtcgtgt gcgagccgtc cgggatcgct gcgattccgc cgccgccgtt
     1201 cgcggctttg ttgtcgctga tcacgctgtc ttcgatgacg aggttgcctt cgtcctggta
     1261 gatcccgccg ccttcgcctt cgcccgccac gttgttggtg acttcggtct cttttagcgt
     1321 caccgtgctg ccggaggcgt cgacgttgat cgccccgccg gaggcttcgg agagactggc
     1381 ttcggtgctg ttcttttcga gcaccgagcc gacaaccgtg aggtcgccgc cctcgacgtc
     1441 gatcgcgccg ccgtcgcggc cgctttcgac cccgttttcg gtcaggcgca gaccttcgag
     1501 ggtgacgtcg ccacccgact ggtagagagc gccgccgccg ctgccgttga tcgcgaagtc
     1561 gtcttcgaag ccttttgcga tcgtcaggcc ggtgatcgtc acgttgccac catggacctc
     1621 gaaggcgcgg tcttttttct ccgcgttgac ggtcaggttg gcattgccgg ggccaacgat
     1681 tttcagcccg gtgatgcccg gtccgggggt cagatcgagc gtgccccggt cgccgatttc
     1741 gatcgtgccg ggggtgacgt cgaaggtgac gagaatctcg ccgctgatta gcggctggcc
     1801 gtgttcgttg agcacgtttt cgccttcggc agtgagttcg agcgcttccc gcagcgaaca
     1861 gccgaagatc gggctggtat tggggccgca gttgccgtcg acgcggtcct gcagggtgtt
     1921 gacgcgaagt tcggcgttgc cgaaggcggc cggcgcaaag gcggccaggc cgagcagggc
     1981 gagtgcaact gcaagcacga agcgaaggcg ggaccggggc gagcggggca tgtctgtttt
     2041 ctggctcctg tagtgggaaa cggcgtgttt ctgacgccga tatcggcatt cggcacgcct
     2101 agctcaaggc ggaaatcgcg cttgcatccg ggaaagtgat tcgctaaact aattatcaac
     2161 gctaactatg aatctcggac ccagcaagaa cctcaccgat tccgccgccc agctgcgcat
     2221 ggcgatcgtg cgcacggccc gccgcctgcg ccaggaggcc gccgccgagg ccagcggtct
     2281 gacgccgacc tcgacctccg cactcgccgc gatcgacgtg cacggcccgc tgacccccag
     2341 cgagctggcc gtgatcgagc gggtcaagcg gccgacgatg acccgcacgc tcggctgtct
     2401 ggagcgggag gggctgatcg aacgcacccc cgaccccgcc gacggccgca gctcgctgat
     2461 cgcgatcaac gccgccggcc gcgagcggct gcgccgcctg cgtgggcgca agaacgccta
     2521 cctggcgcgg cgcatgcgcg acctctcggt agaggaagtg gacacgctgg agcgggcggc
     2581 cgagatcctc gaccggatga gggagggtga gccgcggtcc gggcgcgaga gcgcctcatg
     2641 acgtaccacc tgcgccgctc cttcagctcg ctcgaggttc ccaactaccg gcgctacttc
     2701 gtcggccagc tggtctccct ctcggggacc tggatgcaga acgtcgccgc gatctgggtg
     2761 atcctcaccc tcaccgacaa cggcttcgcg gtcggcctgg cgacggcgct gcagttcctg
     2821 ccgatgctcc tgttcggcgc ctggggcggc ctgctcgccg accggttctc caagcgccgc
     2881 ctgctgctga ccacgcaggt gctgatgatg gtccccgcgg tcggcctcct ggtcgtgacc
     2941 gcgaccggcg tcgtcacccc ctggatggtc tacctcggcg tcttcggctt cggtctgctc
     3001 aacgcgatcg acaacccgac ccggcagagc ttcgtgatcg agatggtcgg cgccgaccgg
     3061 gtcgtcaacg cggtcagcct caacagcgtg atcgtgcagg cggcgcggat cgtcggcccg
     3121 gcgatcgccg ggatcctgat cgccaccgcc ggcgtcgtcc cctgtttcgc gatcaacgcg
     3181 ctgacctcgg tggcgatgat cctggcgctg ctgcggatgg acacgcggcg gctgcacgaa
     3241 ccgccgacgg cgacgcgcga agcgggtgcg atccgcgccg gcctgcgcta cgtccggcgc
     3301 accccggagc tggcggtgcc gctggcgctg atggccctgg tcgggacctt cggcttcaac
     3361 ttccaggtgg tgctgccact gctcgcaaag ttcagcttcg acggcggcgc cgccacctac
     3421 ggggcgctgg tcgcggcgat gggcgtcggc tcgatcgccg gcgccctgat caccggccac
     3481 cagggcaaga cgggcccgcg cctgattgcc gcctccgcgc tcgccttcgg cctctccgcc
     3541 gtgggcgcgg ccgcgatgcc cgccctcgcc ctggagatcc ccctgctggt cctgctcggc
     3601 ggcgccgcgg tcacctttgc ggcgacgatc aactcgagcc tgcagctcgc cgtcgccccc
     3661 gagatgcgcg gccgcgtaat ggccctctac tccgtcgtct tcctcggctc aaccccgatc
     3721 ggagccccgt tggtcggctg gctctcggag gcctacgacc caagagtcgc cctgctcctg
     3781 gcaggaatcg cggggctatc cgcagcgtgg gcggcacacg tttcgtttgc gcgcctgcgt
     3841 gagcgggcgg ggaccgattc ccgcccgctc accgcggcga actagccggt gtaccccgcc
     3901 gattcgaggt attccgccgc gactttcgcc gccggttctt ttt
//
LOCUS       DASRIG010000267         8422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_910338, whole genome shotgun sequence.
ACCESSION   DASRIG010000267 DASRIG010000000
VERSION     DASRIG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910338"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFB52_07305"
     CDS             complement(<1..474)
                     /locus_tag="VFB52_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZO06179.1"
                     /translation="MTLVLLVVLLLLVAVNGFFVAAEFAIVRSRRARLEQMRDEGVAG
                     VSRALDQIEHVDVYVATCQVGITLASLGIGFLGEPALAELFEPLFGNFFSHAVAIVIA
                     IALAFTIVTFLHVILGEQAPKMVAIARAERTLLKIGGPLAFFRRALHPLIVATNAP"
     gene            585..1139
                     /locus_tag="VFB52_07310"
     CDS             585..1139
                     /locus_tag="VFB52_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06180.1"
                     /translation="MRVATLTEMLEEIVRLGEATGMEDHAHELRGELEGRLATVRAAV
                     AGSSSPRVIALERFDPPRAAGYWIPEMISIAGGIDVAGDPGLDPPEVGWGELESLGAD
                     VVIAMPDGSLPDARAQAMEHWERIAALGAARAFAVDADAAFTDAGPRLVDGVELLCHL
                     LHPELIDPPGNAGFEALRAPVTRQ"
     gene            complement(1141..1974)
                     /locus_tag="VFB52_07315"
     CDS             complement(1141..1974)
                     /locus_tag="VFB52_07315"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZO06181.1"
                     /translation="MAEHCYRHPDRETRVSCSSCGRPICPDCMTPTPVGMRCPECSAQ
                     KTRVVRNPSGAPQSGFMGSPATYVLIAINVVAYLIEIASGGGGFTTGSANSVTVDFGL
                     FGPLVAEGEWYRIITAGFLHVNLIHIGFNMFLLFMLGRLIEPSLGTPRFLLVYFVSLI
                     AGSLGALILDPNALTIGASGAVFGLFGASFLLARERGMNALAGELGLLIVINLVFTFA
                     SSRNISVGGHLGGLAGGAICAALIIAGARGMLGPNHRVVEYVGLVAVGVAAFVGCIAV
                     A"
     gene            2004..2384
                     /locus_tag="VFB52_07320"
     CDS             2004..2384
                     /locus_tag="VFB52_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06182.1"
                     /translation="MEDASPARLYASALGALLLVIGIAGFFGAVEVDAWRNLLYAATG
                     ALGLLAASYGARPFALIAGLLYTVLAIRGFTLPNGEEVLDLFPSDQLEDCLRLAVGLL
                     GLAAAAGTPRARLKARAHAAREGA"
     gene            complement(2346..2696)
                     /locus_tag="VFB52_07325"
     CDS             complement(2346..2696)
                     /locus_tag="VFB52_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06183.1"
                     /translation="MSEPSESLSGNWDVEQLLSDALRPIEPPERLSGRIEDTFAAITQ
                     TAAEELSAWAEELSESELRALRDPRNWVRPVVAVSAGGIATGALVVLELRRRSKQKSE
                     SGLRALSSSVRSRL"
     gene            complement(2680..3249)
                     /locus_tag="VFB52_07330"
     CDS             complement(2680..3249)
                     /locus_tag="VFB52_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO06184.1"
                     /translation="MSGPEENVEPAAMAPAAKAELDRAFEEFYRSHLRDVYSYAYYRV
                     GNHHDAEDLTEQAFLQAYRHFDRARRESDGRPLRPWLIRIAHNLASNYYRDRSRRPQT
                     PLENADPIAAKHDTEAIAEGREELRQVMANLEHLSEDRREALIMRFALGMDNREIARA
                     LGRSDGATKVLIHRAIKQLQEEMDDERAE"
     gene            complement(3268..4932)
                     /locus_tag="VFB52_07335"
     CDS             complement(3268..4932)
                     /locus_tag="VFB52_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZO06185.1"
                     /translation="MTAADLAKYHEYTRKHSVNWPLYILARIFLTPFFLVYFRYARTG
                     RRYARLKGGMIVAANHRSFLDPFVVGGCLPWGKPMNYVAKQELFGNPLQAWFLSRLGA
                     FPIRRGESDEASMETARRVVERGGAVCIFPEGTRHRRGTLGNPKRGVGRLALQTGAPV
                     VPTAVIGTEHVRRGWRIRPRKVKVRLGKAMTFPRAEEPSPALAETVTARIWPNVQLQW
                     EDLGGLPPMRRAAVIGAGSWGTAVAVLLARGGLEVQLGARTPESVAELIEAGENARYL
                     PGVPLPESIDVRQASEIELDGLDLICLAIPSKSLPQAVGAIADRVGGRSSILLLTKGL
                     VGPQGQLPASYVGERVRARAIACLGGPAHARESVSGSAALVLGSADEDLRTQLGEVFD
                     KAGLVCERTADVTGVEMAGAAKNAAALAAAAAESHGLNAAGIAVAEIWSECIEYALAR
                     GAALETFSGLAGVGDLTATMMAPTGRNRRAGELLGSGTPAAEIPAIIGQASEGLDTTP
                     LLAETVAASGVSAEALSGLAELIRGEIDADAWVAGLRRVERARRAA"
     gene            complement(4965..6002)
                     /locus_tag="VFB52_07340"
     CDS             complement(4965..6002)
                     /locus_tag="VFB52_07340"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZO06186.1"
                     /translation="MGRGFVWRDAGRTVVFGEGGVERAVEVFAEHGVADFELLSTERA
                     LAGAPKLAEAASAVHEVPSGQVPDLAATLLQPEVALLAGMRQEVRPQMVALGGGRVVD
                     VVKAVASVTGADVIAIPTTMSGAEMTAIHRLPAGAESRAQGMVRPRLVIADPAAMTSQ
                     PEAALRASSMNALAHGADSLYTPFTNPLSEHAAVQGAGLIATALDQDPAKRDRAGLAL
                     GSILCGYAIDSAGLGLHHVICQTLVRLTGTPHAETNAAILPPALAFLASRAPQAFESL
                     AAALGTDLDGLAPRITALGQPPALAPLSTQPENLEPALEAMLKRSELSHVPCPAVTHE
                     DLAGIVAAAWK"
     gene            complement(6002..6913)
                     /locus_tag="VFB52_07345"
     CDS             complement(6002..6913)
                     /locus_tag="VFB52_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06187.1"
                     /translation="MKLEGIHHITAITENAQRNVDFYAGVLGLRLVKKTVNQDNPTVY
                     HLFFADEDGDAGSDLTFFEYPGAAQGRPGAGMIHRIVWRVASTDSLDFWETRLATNDV
                     SSRREGGSLHFSDPEGLDHELAVVTVEDAPLIADNPEIPGELALQGFHAARAYATAPE
                     ASSELLEALEFERVEGGAWEARGDQRGGLYFYDEPPAERGVQGAGSVHHIAWASTIDE
                     HMQWRQRAIDGGASPTPEIDRFYFKSIYFREPSGVLFEIATLGPGFTVDEPLESLGEK
                     LSLPPDFEHLRDEVEPNLRPVVNPRAR"
     gene            6939..8348
                     /locus_tag="VFB52_07350"
     CDS             6939..8348
                     /locus_tag="VFB52_07350"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO06188.1"
                     /translation="MSRARRRTAKGLIILASVFAFLSVFGIWVERQALNTDDWVDTSG
                     RLIENDKIRTAVADYIVDELYENVDIKKELEDIFPGETKDLAGPAAGALRQVAGDGAE
                     EVLKTSTAQSLWRDANRTAHEQLMAVIEDKKDAVSTENGEVSLNLGSLVTNLANQVGI
                     GASLAEKLPADAGQIKILESDELKTAQNIVVAIKGLALVLSILTFACFALAIYLYKES
                     RWIAVLFAGVGLIAAGFAVIVARQIAGGIVIDQLVTDETVKPAAEAAWSIATSLMVSI
                     ATTVIVVGVLFAVAGWLASPHATSRATRKVIAPALRDYSPWVYTGLAIIVSIYFLSSS
                     VQSLRSFVTTLIVALMAAAWIHELRKQCIEEFPDAKFSDTFGGPRDRVVSAVKGANLG
                     ERAAKLGESAKKVRLPEVPRSDGGETPTSEAPTTEAPTAVLGEDQRLARLERLGELRE
                     KGILSDEEFAAEKARVLGD"
BASE COUNT         1289 a         2916 c         3008 g         1209 t
ORIGIN      
        1 cggcgcgttg gtggcgacga tcagcgggtg cagggcgcgg cggaagaagg cgagtggccc
       61 gccgattttc agcagcgttc tctcggcccg cgcgatcgca accatcttcg gcgcctgctc
      121 gccgaggatc acgtgcagga aggtgacgat cgtgaaggcg agggcaatcg cgatcacgat
      181 cgccacggcg tgtgagaaga agttgccgaa cagcggctcg aacagctcgg ccagggccgg
      241 ctcgccgagg aagccgatgc cgagcgaggc gagcgtgatc ccgacctgac aggtggcgac
      301 gtagacgtcg acgtgctcga tctggtcgag cgctcgcgat accccggcga cgccttcgtc
      361 gcgcatctgc tcgagccggg cccggcgcga gcgcacgatc gcgaactcgg ccgcgacaaa
      421 gaagccattg accgcgacca acagcaggag gacgaccagg aggacgaggg tcatcgctta
      481 tgcggccctg gtacttcggg ctgggacgtc atcggccact tgagcgaacg gaatatagct
      541 ggccgccccg cggcaccggc gagagatggg atattgggca aacgatgcgg gtcgccacgc
      601 tgaccgagat gctcgaagag atcgtccggc tgggcgaggc gacgggcatg gaggaccacg
      661 cccacgagct gcgcggcgaa ctggagggac ggctggcgac ggtgcgggcg gcagtcgcgg
      721 ggtcctcgag cccacgggtg atcgcgctcg agcgcttcga ccctccgcgc gcggccggct
      781 actggatccc ggagatgatc tcgatcgccg gcggcatcga cgtcgccgga gaccctggcc
      841 tcgacccccc cgaggtcggc tggggcgagc tcgagagcct tggcgcggac gtggtgatcg
      901 cgatgcccga cggctcgctc cccgacgctc gcgcccaggc gatggagcac tgggagcgga
      961 tcgccgcgct cggcgccgca cgcgccttcg cggtcgacgc cgacgccgcc ttcaccgacg
     1021 ccggcccgcg cctggtcgac ggcgtcgagc tgctctgcca tctgctccac cccgagctga
     1081 tcgacccgcc cggcaacgcc gggttcgaag ccctgcgcgc accggttacg aggcagtagc
     1141 tcaggcgacg gcgatacagc cgacgaaggc ggcgacgccg acggcaacca gacccacata
     1201 ctcgacgacc cggtggttgg gtccgagcat gccgcgcgca ccggcgatga tcagcgcggc
     1261 acagatcgct cccccggcca agccaccgag gtggccgccg acgctgatgt tccgcgagct
     1321 ggcaaaggtg aagacgaggt tgatcacgat cagcagcccg agctctcccg cgagcgcgtt
     1381 catcccgcgc tcccgtgcga gcaggaagct cgccccgaag agcccgaaga ccgcccccga
     1441 tgccccgatc gtgagcgcgt tcgggtccag gatcagcgcg ccgagcgagc cggcgatcag
     1501 cgagacgaag tagacgagca ggaagcgcgg cgtgcccaag gagggctcga tcagacgccc
     1561 gagcatgaac aacaggaaca tgttgaaccc gatgtgaatg aggttcacgt gaaggaagcc
     1621 ggcggtgatg atccggtacc actcgccttc cgcgaccagc gggccgaaga gaccgaagtc
     1681 gacggtgacg ctgttggcac tgccggtggt gaacccaccg ccccccgagg cgatctcgat
     1741 caggtatgcg accacgttga tcgcgatcag cacgtaggtc gcgggcgacc ccataaagcc
     1801 agactgcggc gccccgctcg ggttgcgcac tacccgggtt ttctgcgcgc tgcactccgg
     1861 gcagcgcatc ccgaccgggg tcggcgtcat gcagtcgggg cagatcgggc gaccgcagga
     1921 agagcaggaa accctggtct cgcggtcggg gtggcggtaa cagtgttcgg ccattcggtc
     1981 gagggagcct acaatcggcg ccgatggagg acgccagccc cgcccgtctc tacgcgtcgg
     2041 cgctcggcgc gctgctgctt gtgatcggga tcgcgggctt cttcggcgcg gtcgaggtgg
     2101 acgcctggcg caacctcctc tacgcggcga ccggcgccct cggcttgctc gcggccagct
     2161 acggcgcgcg cccctttgcc ctgatcgccg gcctgctcta cacggtgctg gcgatccgag
     2221 gattcacgct ccccaacggc gaggaggtcc tcgacctgtt ccccagcgac caactcgagg
     2281 actgcttgcg gctcgcggtc ggcctcctcg ggctcgccgc cgcggccggc acgccgcggg
     2341 cccgactaaa ggcgcgagcg cacgctgctc gagagggcgc gtaggccgct ctcgctcttc
     2401 tgcttgctgc gccgccgcag ctccagcacg accagcgcgc cggtggcgat cccgccggcg
     2461 ctgaccgcaa ccaccggccg cacccagttg cgcgggtcgc gcagcgcccg cagctcgctc
     2521 tcggagagct cctcggccca ggccgagagc tcctcggccg cggtctgggt gatcgccgcg
     2581 aaggtgtcct cgatccggcc cgagagccgc tcgggcggct cgatcgggcg cagcgcgtcg
     2641 ctgagcagct gctcgacgtc ccagttgccg ctcaggctct cactcggctc gctcatcgtc
     2701 catctcctcc tgcaactgtt tgatcgcgcg gtggatcagc accttggtcg ccccgtccga
     2761 gcgccccagc gcccgggcga tctcgcgatt gtccatgccc agggcgaagc gcataatcag
     2821 cgcctcgcgc cggtcctcgg agaggtgctc cagattggcc atcacctggc gcagctcctc
     2881 gcgcccctcg gcgatcgcct cggtgtcgtg cttggccgcg atcgggtcgg cgttttccag
     2941 cggcgtctgc gggcgccgcg agcggtcgcg gtagtagttc gaggcgaggt tgtgggcgat
     3001 ccggatcagc cacggccgca gcggccgccc gtcggactcg cgccgcgccc ggtcgaagtg
     3061 gcggtaggcc tgcaggaagg cctgctcggt gaggtcctcg gcgtcgtggt ggttgcccac
     3121 ccggtagtag gcgtaggagt acacgtcgcg cagatgcgag cggtagaact cctcgaaggc
     3181 gcggtccagc tccgccttcg ccgcgggcgc catcgccgcc ggctcgacgt tctcctcggg
     3241 gccgctcacc gtccaagcct attggcatca cgccgcgcgc cttgcccgct cgacccggcg
     3301 caagccggcc acccaggcgt cggcgtcgat ctcgccgcgg atcagctcgg ccagtccgct
     3361 gagcgcctcc gccgagacgc ccgaagcggc gacggtctcg gccagcagcg gcgtcgtgtc
     3421 gaggccctcc gatgcctggc cgatgatcgc cgggatctcg gccgccgggg tgcccgaacc
     3481 gagcagctcg ccggcccgcc ggttgcgccc ggtcggcgcc atcatcgtcg cggtcaggtc
     3541 gccgaccccg gcgaggccgg agaaggtctc cagcgcggcg ccgcgggcga gcgcgtactc
     3601 gatgcactcg ctccagatct cggcgaccgc gatcccggcg gcgttgaggc catggctctc
     3661 ggccgcggcg gcggcgagcg cggcggcgtt cttcgccgcc ccggccatct ccaccccggt
     3721 tacgtcggcg gtgcgctcgc agaccaggcc ggccttgtcg aagacctcgc cgagctgggt
     3781 gcgcaggtcc tcgtcggccg agcccaggac cagcgccgcc gagccggaga ccgactcacg
     3841 tgcgtgggcg gggccgccga ggcaggcgat cgcgcgggcg cgcacccgtt cgccgacgta
     3901 agaagccggc agctggccct gcgggccgac caggcccttg gtcaagagca ggatcgagga
     3961 gcgaccgccg acccggtcgg cgatcgcgcc gaccgcctgc ggcaacgatt tcgaggggat
     4021 cgcgaggcag atcaggtcga ggccgtcgag ctcgatctcg ctcgcctggc gcacgtcgat
     4081 cgactcgggc agcggcacgc cgggcaggta gcgggcgttc tccccggcct cgatcagctc
     4141 ggcgacgctc tcgggcgtgc gcgcgccgag ctggacctcg agcccaccgc gggcgagcag
     4201 gaccgcgacc gcggtccccc agctgccggc gccgatcacg gcggcgcggc gcatcggcgg
     4261 cagcccaccg aggtcttccc actgcagctg cacgttcggc cagatccgcg cggtcacggt
     4321 ttcggcgagc gcgggcgagg gctcctcggc gcgcgggaag gtcatcgctt tgccgagccg
     4381 gaccttgacc ttgcgggggc ggatccgcca accgcggcgc acgtgttcgg tcccgatcac
     4441 cgcggtcggg acgaccgggg cgccggtctg cagggcgagc cggccgaccc cgcgcttggg
     4501 gttgccgagc gtgccgcggc ggtggcgggt gccctcgggg aagatgcaga cggcgccgcc
     4561 gcgttcgacc acccggcgcg cggtctccat cgacgcctcg tcggactcgc cgcggcggat
     4621 cgggaaggca ccgagccgcg agaggaacca ggcctgcagc gggttgccga acagctcctg
     4681 cttggcgacg tagttcatcg gcttgcccca gggcagacag ccgccgacca cgaacgggtc
     4741 gaggaagctg cggtggttgg cggcgacgat catcccgccc ttgaggcgtg cgtagcggcg
     4801 gccggtccgc gcgtagcgga agtagacgag gaaaaacggc gtcaggaaga tcctggccag
     4861 gatgtagagc ggccagttga cgctgtgctt gcgggtgtac tcgtggtact tggcgaggtc
     4921 ggcggcggtc acgctctgcg tgctacacgg ctggggggat ccggttactt ccaggcggcg
     4981 gcgacgatac cggcgagatc ctcatgggtg acggcggggc aggggacgtg gctcagctcg
     5041 gagcgcttca gcattgcttc gagggcgggc tccaggttct ctggctgggt agagagcggc
     5101 gcgagagcgg gcggctggcc aagggcggtg atccggggcg cgaggccgtc gaggtcggtg
     5161 ccgagggcag cggcgagcga ctcgaaggct tgcggcgcgc gggaggcgag aaaggcgagc
     5221 gctggcggga ggatcgccgc gttggtctcc gcgtgtgggg tgccggtgag gcggaccagg
     5281 gtctggcaga tcacgtggtg caggccgaga ccggccgagt cgatcgcgta gccgcagagg
     5341 atcgagccga gcgcgaggcc ggcccgatcg cgcttcgccg ggtcctggtc gagcgctgtg
     5401 gcgatcagac cagctccttg gacagcggcg tgctcggaga gcgggttggt gaacggcgtg
     5461 tagagcgagt cggcgccgtg cgcgagcgcg ttcatcgagc tcgcccgcag cgccgcctcc
     5521 ggctggctcg tcatcgccgc cgggtcggcg atcaccaggc gtgggcggac catgccctgc
     5581 gcccgcgact cggccccggc cggcagccgg tggatcgcgg tcatctccgc ccccgacatc
     5641 gtcgtcggga tcgcgatgac gtcggcgccg gtcaccgagg cgacggcctt gaccacgtca
     5701 acgaccctcc ctcccccgag cgccaccatt tgcggtcgca cttcttgccg cataccggca
     5761 agaagtgcga cctcaggctg aaggagggtg gcggcgaggt cggggacctg accggacggg
     5821 acctcgtgaa cggcactcgc agcttccgcg agcttcgggg cgccggcaag cgcccgctcg
     5881 gtgctcagca gctcgaaatc ggcaacgccg tgctcggcga agacctcgac cgcccgctcg
     5941 accccgccct cgccgaagac gacggtccgc ccggcgtcgc gccagacgaa cccgcggccc
     6001 actaccgggc gcgcgggttg acgaccgggc gcaggttcgg ttcgacctcg tcccgcaggt
     6061 gctcgaaatc gggcggcagc gagagctttt cgccaaggct ctcgagcggc tcgtcgacgg
     6121 tgaagcccgg cccgagcgtg gcgatctcga agagcacgcc ggagggctcg cggaagtaga
     6181 tcgatttgaa gtagaagcgg tcgatctccg gggtcggcga ggcgccgccg tcgatcgccc
     6241 gctgacgcca ctgcatgtgc tcgtcgatcg tcgacgccca ggcgatgtgg tggacgctgc
     6301 cggcgccctg cacgccgcgc tcggccggcg gctcgtcgta gaagtagagg ccgccgcgct
     6361 ggtcgccgcg cgcctcccag gcgccgccct cgactcgctc gaactcgagc gcctcgagca
     6421 gctcgctgct cgcctccggc gcggtcgcgt aggcgcgggc ggcgtggaag ccctgcaggg
     6481 cgagctcgcc ggggatctcc gggttgtcgg cgatcagcgg cgcgtcctcg accgtgacca
     6541 cggcaagctc atggtcgagc ccctcgggat cggagaagtg caggctcccg ccctcccgcc
     6601 gtgaggagac gtcgttggtg gcgagccgcg tctcccagaa gtcgagcgag tcggttgagg
     6661 cgacccgcca gacgatccgg tggatcatcc cggcaccggg ccggccctgg gccgcgccgg
     6721 gatactcgaa gaaggtcagg tcagagccag cgtcgccgtc ctcgtccgcg aagaacaggt
     6781 ggtagacggt cgggttgtcc tggttgacgg ttttcttgac cagccgcagt cccagcacgc
     6841 ccgcgtagaa gtcgacgttg cgctgggcgt tctcggtgat cgccgtgatg tggtgaattc
     6901 cctctagctt catgcgccac gtatagcatc acttcgcaat gagccgcgca cgccgacgta
     6961 ctgccaaggg gctcatcatt ctggcgtcgg tgttcgcctt cctcagcgtc ttcgggatct
     7021 gggtcgagcg ccaggcgctc aacaccgacg actgggtcga caccagcggg cggctgatcg
     7081 agaacgacaa gatcaggacc gccgtcgccg actacatcgt cgacgaactc tacgaaaacg
     7141 tcgacatcaa gaaagaactc gaggacatct tcccgggcga aaccaaggac ctcgccggac
     7201 cggccgccgg ggcgctgcgc caggtcgccg gcgacggcgc cgaagaagtg ctgaagacgt
     7261 cgaccgcgca gtcgctgtgg agggacgcca accgcaccgc ccacgaacag ctcatggcgg
     7321 tgatcgaaga caagaaagac gcggtctcga ccgaaaacgg cgaagtcagc ctcaacctcg
     7381 gcagcctcgt caccaacctc gccaaccagg tcgggatcgg cgcctcgctg gcggaaaaac
     7441 tgccggccga cgcgggccag atcaagatcc tcgaatcgga cgagctgaag accgcgcaga
     7501 acatcgtggt cgcgatcaag ggcctggcgc tggtcctctc gatcctcacc ttcgcctgct
     7561 tcgcactggc gatctacctc tacaaggaga gccgctggat cgcggtcctg ttcgccgggg
     7621 tcggcctgat cgccgccggg tttgcggtga tcgtcgcccg ccagatcgcc ggcgggatcg
     7681 tgatcgacca actggtcacc gacgaaaccg tgaagccggc ggcggaagcc gcctggtcga
     7741 tcgcgacctc gctgatggtc agcatcgcga ccacggtgat cgtggtcggc gtgctgttcg
     7801 cggtcgccgg ctggctcgcc tccccgcacg cgacctcgcg cgccacccgc aaggtgatcg
     7861 ccccggcgct gcgcgactac tcgccctggg tctacaccgg cctcgcgatc atcgtcagca
     7921 tctacttcct ctcctcctcg gtgcagagcc tgcgctcctt cgtcaccacc ctgatcgtcg
     7981 ccctgatggc cgcagcctgg atccacgagc tgcgcaaaca gtgcatcgag gagttccccg
     8041 acgccaagtt cagcgacacc ttcggcggac cgcgcgatcg ggtcgtgagc gcggtcaaag
     8101 gagccaacct cggcgagcgg gcggcgaagc tgggcgagag cgcgaaaaaa gtgcgcctgc
     8161 ccgaggtgcc gcgctccgac ggtggtgaga cgccgacgag cgaggccccc accaccgagg
     8221 cgccgacggc tgtgctcggc gaggaccagc gcttggcccg gctggagcgg ctcggtgagc
     8281 tgcgcgagaa ggggatcctc agcgacgagg agttcgccgc cgagaaggcc cgcgtcctcg
     8341 gcgattagcc ccgatcggct agcctcgtca gcatggactt cgatttccgc ttccgcaatt
     8401 cgcacggcaa atgggagtcg ag
//
LOCUS       DASRIG010000268         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1953484, whole genome shotgun sequence.
ACCESSION   DASRIG010000268 DASRIG010000000
VERSION     DASRIG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953484"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VFB52_07355"
     CDS             complement(<1..169)
                     /locus_tag="VFB52_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZO06189.1"
                     /translation="MFKKVLIANRGEIALRVMWACRELGIKTVAVYSESDRDSLHVRF
                     ADQAVCIGPPRS"
     gene            complement(174..650)
                     /gene="accB"
                     /locus_tag="VFB52_07360"
     CDS             complement(174..650)
                     /gene="accB"
                     /locus_tag="VFB52_07360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZO06190.1"
                     /translation="MDLKELKQLLQILEEKQIAEFEIEEAGIKLRIKKAGAATVITGP
                     AAVPIPAPVAPPVPSAEPTTEPGPTAVSKPVEAGLTIVKSPIVGTFYRSPDPNAAPFV
                     SVGDSVKVGQKLCIIEAMKLMNEIESELAGEVVEVYPENGQPVQYGEPLLSIRPSR"
     gene            complement(705..1082)
                     /locus_tag="VFB52_07365"
     CDS             complement(705..1082)
                     /locus_tag="VFB52_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06191.1"
                     /translation="MYAVVTLYVSISDASQVRFIDIDPLPNGSFVAVMPSGSFTGVNL
                     GTACGVGPALTFDPNATRQLSAGVSPYSGTFQAFLGSPDLRNIGSSASLTGPWVLEVG
                     SGAGPLPVSTTINCWQLDVYTTP"
     gene            1114..1464
                     /locus_tag="VFB52_07370"
     CDS             1114..1464
                     /locus_tag="VFB52_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06192.1"
                     /translation="MGAESTVFEPVVTTTGVEFVNVCLVGGGVGGDDGVPDGEPDGPG
                     VGVPDGPGVGGPFGAGDVRTLPGDDRMSLGDGRMPLGDDRMSLGDAMIVACPSELMPA
                     VEGLVTSSFAVHAR"
     gene            complement(1535..1894)
                     /locus_tag="VFB52_07375"
     CDS             complement(1535..1894)
                     /locus_tag="VFB52_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZO06193.1"
                     /translation="MAFGDALQRISSRVPEAHVLMIMGVDGIPIEKLVVRQTANLEAV
                     AAEYTTLLRASVSAATDTGLGELLELAVVTEKMTTLLIGITAEYFLFAALAPGALVGR
                     ARFVLRMAGLDLQKEFD"
     gene            complement(1899..>2920)
                     /locus_tag="VFB52_07380"
     CDS             complement(1899..>2920)
                     /locus_tag="VFB52_07380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO06194.1"
                     /translation="EGLQKHPQYPSARMTLGRALFDTGDLAAARTEFQAVLKGAPDNI
                     LAARLLGESLEGLEDWLAAAAQYKRALMMAPGDKQLQAHLEAVENRIREPEPEAARHD
                     EAEPAPIKVLTVDEPMELERPFESRATQVGEAPEPPEFTGTMAIPVAQVEEDFEIERP
                     VEAPSVSLKPAPPPPPPVRIAPPPPEEVVEFDEAPTMPGTLPATPDIEYEPTTLPPQA
                     VVDAAPREVAPEAPFVASEPSIAEPAPLIDVEPEAPSEEIASVTLADLYSRQGFNDKA
                     AQVLEQLLLREPGNERAQARLREIRSSAAGAMPPPADATDAKRVVIQRTIDRLEAMLA
                     AIRKG"
BASE COUNT          441 a          947 c          998 g          534 t
ORIGIN      
        1 tgctgcgcgg cggcccgatg cagaccgcct ggtcggcgaa ccggacgtgc aggctgtcgc
       61 ggtcgctctc ggagtagacg gccaccgtct tgatgccgag ctcgcggcac gcccacatca
      121 cgcgcagggc gatctcaccg cgattcgcga tcaggacctt cttgaacaac ggcttagcgg
      181 ctgggccgga tcgacaggag cggctcgccg tactgaacgg gctgcccgtt ctcggggtag
      241 acctcgacga cttcgccggc cagctccgac tcgatctcgt tcatcagctt catggcttcg
      301 atgatgcaga gcttctggcc gaccttgacg ctgtcgccga cgctgacgaa aggcgcggcg
      361 ttcgggtcgg gcgagcggta gaaggtcccc acgatcgggc tcttgacgat cgtcagaccg
      421 gcctcgaccg gcttgctgac ggccgtgggg ccgggttccg tggtgggctc ggcgctcggc
      481 accggcgggg caaccggcgc cggtattggt accgccgccg gtccggtgat gacggtggcc
      541 gcccccgcct ttttgatccg gagcttgatc ccggcttctt cgatctcgaa ttcggctatc
      601 tgcttctctt cgaggatttg gagaagctgc ttcagttcct tgaggtccac gtaaggctag
      661 attcttcttg cctgacgcgg acttgtcaaa cggacattgc ttgcctacgg ggtcgtgtag
      721 acgtctagct gccagcagtt gatggtcgtg gagacgggca gcggcccggc tcccgagcca
      781 acctcgagaa cccaggggcc cgtcaacgaa gccgaggacc ctatgtttct gaggtcaggc
      841 gagccgagaa acgcctggaa cgttcccgag taggggctga ctcctgcgct gagctggcgg
      901 gtcgcattcg ggtcgaacgt cagagccggc ccaaccccgc aggcggtgcc gaggttgact
      961 cccgtgaaac tcccgctggg catgacagcc acaaagctac cgttgggcag ggggtcgatg
     1021 tcgatgaatc taacctggct ggcgtcgctg atcgatacgt aaagggtcac gaccgcatac
     1081 acgacgcttc ctgtaccaag accggtcaca ttgatgggcg cggaatcgac agtgttcgaa
     1141 ccggtagtga ccaccaccgg cgttgagttc gtgaatgtgt gcttggttgg gggcggagtc
     1201 gggggcgacg acggcgttcc tgacggcgag ccggatggcc ccggcgtcgg cgttcccgac
     1261 ggaccgggtg tagggggtcc gttcggcgca ggcgacgtgc gaacgttgcc cggtgacgac
     1321 cggatgtcgc tcggcgacgg ccggatgccg ctgggagatg accgaatgtc gttgggcgac
     1381 gccatgatcg tggcctgccc ctcggagctg atgccggccg tcgaggggct cgtgacctcg
     1441 tcttttgccg tgcatgcgag gtagaagccc acgagcaggc agagcgcggt gattcgcgtc
     1501 agcgtgctcg ccggtccgag atttgttcgt gccatcagtc gaactccttt tgcagatcca
     1561 acccagccat cctcaagacg aatcgcgctc gacctacgag tgcgcccggc gcgagggccg
     1621 cgaagagaaa gtattcggcg gtgatcccga tgaggagggt ggtcatcttc tccgtcacaa
     1681 ccgccaattc gagcagctcg cccaggccgg tgtctgtggc cgcggacaca ctggccctga
     1741 gcagcgtcgt gtactcggcc gcaaccgctt ccaggttagc ggtttgccgg acgaccagct
     1801 tttcgatggg aatgccgtcc acgcccatga tcatcagcac atgggcttcg ggcactcggc
     1861 tcgagatccg ttgcagcgcg tcaccaaaag ccaaggaact atccctttct gatcgcggcc
     1921 agcatcgcct cgagccggtc gatcgtgcgc tggatcacca cgcgcttcgc gtcggtcgcg
     1981 tcggccgggg gcggcatggc gcccgcagcc gagcttcgga tctcgcgaag ccgcgcctgg
     2041 gcgcgctcgt tccccggctc ccgtagcaag agctgctcca ggacttgggc ggccttgtcg
     2101 ttgaagcctt ggcgcgagta gaggtctgcc agggtgaccg atgcaatctc ttctgagggc
     2161 gcctcgggtt ctacgtcgat gagcggcgcc ggctcggcga tcgacggctc gctcgccacg
     2221 aagggcgcct ccggcgccac ctcgcgagga gcggcgtcga ccaccgcttg gggaggcagt
     2281 gtcgtcggct cgtactcgat gtcgggcgtc gccggcagcg tgccgggcat cgtcggtgct
     2341 tcgtcgaact cgacgacctc ttcgggaggc gggggagcga ttcgcaccgg gggcggcggc
     2401 ggcggagccg gcttcagcga gacgctcgga gcctcgaccg gccgttcgat ttcgaagtcc
     2461 tcttcgacct gggccaccgg gatcgccatc gtgcccgtga actcgggcgg ctccggggcc
     2521 tcgccgacct gggtcgctcg ggactcgaac ggacgctcca gctccatcgg ctcgtccacc
     2581 gtcaggacct tgatcggcgc cggctccgcc tcgtcgtgcc gcgcggcctc gggctccggc
     2641 tcgcgaatcc ggttctccac ggcctccagg tgcgcctgga gctgcttgtc gccgggagcc
     2701 atcatcaggg cccgcttgta ctgcgccgcg gcagcgagcc aatcctccag gccttcgagg
     2761 gactctccca agagccgcgc agcgaggatg ttgtccggcg cgcccttcaa gaccgcctgg
     2821 aattcggtcc gcgccgccgc caggtcgccg gtgtcgaaga gcgcccggcc cagggtcatc
     2881 cgcgcggacg ggtactgcgg gtgcttctgc aggccttcac
//
LOCUS       DASRIG010000269         7803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_383037, whole genome shotgun sequence.
ACCESSION   DASRIG010000269 DASRIG010000000
VERSION     DASRIG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383037"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1252)
                     /locus_tag="VFB52_07385"
     CDS             complement(11..1252)
                     /locus_tag="VFB52_07385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06195.1"
                     /translation="MLQEVALGQKSLTDYTHIVGKELTEQIRELAEPLKGKRVLHVSA
                     TAFGGGVSEILYTIVPLMRDVGLDAHWHVIFGKEEFFNATKLLHNSLQGAEETLSDEQ
                     WEIFDEINQINAEGLKGEWDTVIVHDPQPIGLHKGAKEKGNNWIWRCHIDLSTPNPAP
                     IERLLPMIEEYDASVWHLPTYVPAAMDGARAGVNIIPPAIDPLSPKNMAFSPEDAAFV
                     CRQFGIDVERPLITQVSRFDPWKDPIGVIDAYREITEQVPEAQLAMVGSMATDDPEGR
                     EYFQKTFEYADADPDIKILSNLNNVGAIEVNAFQSQSDVCLQKSIREGFGLTVTEALW
                     KGRPTVAGNVGGIPLQIEDGISGYLVNSPDECADRCLEILGNPSLGKSLGREGKEHAR
                     RQFLSPRLLRDWLDLFTRLES"
     gene            complement(1303..2442)
                     /locus_tag="VFB52_07390"
     CDS             complement(1303..2442)
                     /locus_tag="VFB52_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZO06196.1"
                     /translation="MRARAALAALLLLGLGLLAGCGSGSDETSAGTETAEEVPTRVIV
                     ETATSGFDAAAVYRKASPGVVTIRSLFDSGAAEGSGFVLNADGEIVTNAHVVTEEGTG
                     SRRPAEEVFVEFPDRNVVAAEIVGFDPFVDVALLRVEPGDVPLHPLALGDDAELQVGQ
                     PVAAIGSPFGEQQSLSVGVISATDRSVRSLTEFQIEGAIQTDASINPGNSGGPLLDAA
                     ARVIGINQQIETESGANDGVGFAVPISSIRRSVAQLEADGSAEYAYIGVSSQALYPQL
                     AKKLGLDTNFGGLLATVVPGGPAEKAGLRGGDENLQFQAGEYSTGGDVILEVDGHKVV
                     GPEDLSRLISTFQPGDKVKLTVLRDGDKDEVEVTLGRRPDAVPAG"
     gene            complement(2439..3422)
                     /locus_tag="VFB52_07395"
     CDS             complement(2439..3422)
                     /locus_tag="VFB52_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO06197.1"
                     /translation="MTIAGGASGLHSVKLQIVVGGLIAAALATILGVYYAGVAVSNVR
                     RLQDAARKVADGNFETTIPVASVGQLAPLARTFNEMQKRLYELDSARKQFIANASHEL
                     RTPIFSLGGFVELLDEENPSPEERAEFVREMRQQIERLTKLTADLLDLSQLDAGAMVM
                     QTRSVDLSGLAREVTKEFGARAELRGSRLELRAPERPVIARGDPDRVRQIIRILLDNA
                     LTHTPEGTKVTVTTYSENRRAELTVADEGEGIPQRVQNRIFERFYTADSAGGSGLGLA
                     IASELAQRMRGRITISSSRSRNSFTAFTLDLPPARQEGAAQVPRAATKAAT"
     gene            complement(3522..4220)
                     /locus_tag="VFB52_07400"
     CDS             complement(3522..4220)
                     /locus_tag="VFB52_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06198.1"
                     /translation="MANGSARILLVDDEQSIQTLLSYPLRKDGYHVTSALDGSEALQR
                     FEDGRFDLVILDLMLPRLDGVEVCRQLRSRSQVPIIMLTAKGSETDKVAGLEVGADDY
                     ITKPFSMREFRSRVKAALRRSRMGSPEQEDEGAIESGALKIDFDRRMVTLGEGEIGVT
                     YVEFEILGALARSPGRVLTRETLLEHVWGDSEYRDPRTVDVHIRHLREKLEADPKQPE
                     YLFTVRGVGYRFKE"
     gene            complement(4477..5460)
                     /gene="thiL"
                     /locus_tag="VFB52_07405"
     CDS             complement(4477..5460)
                     /gene="thiL"
                     /locus_tag="VFB52_07405"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZO06199.1"
                     /translation="MGEFELLAKLRERLPAPGPRVRLGSGDDAAVTVPGGATATSVDA
                     IVDGVHFQREQSDPEAIGHKALATALSDLAAMGAEPGEAYVYLGVPAEVDEDECLRLL
                     DGMAALARATGTTLAGGDVTRAPALTLAVTVVGHAEAPEQLVARAGAAPGDVLVLTGE
                     LGGAAAGLLLLKRPELAAAVPAETAARLRRRQQRPQPRLRAGRGLAAAGASAMIDLSD
                     GLGADAGHLAAASGVALRIDAAGLPLAKGVAEVALAAGQDPLELAASGGEDYELFAAL
                     PPARLGDASSAIGEAEEAVLTPVGEVAAGSGVEIRLPGGGDLKPDGFDQLR"
     gene            5534..6622
                     /locus_tag="VFB52_07410"
     CDS             5534..6622
                     /locus_tag="VFB52_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZO06200.1"
                     /translation="MDLEDREPLLHRIGEAGARVGSRPPRSRRVRLALQIGLAVLIFG
                     FLVLTVIDQWSEIKEEGVHFHVGWLAPALVILPFFYVFSAYGWDLILRFMGVRIGFGR
                     AQVAWGQPLLARYVPGSVLYVLGRVVLSERAGIPRRMTIASIVYEQAISATSAVVVAS
                     YFIINHPDLEGEPWRWAVLLMIPLAIVILQPRVFGPLANKALRAFGREPLPAVVPLHG
                     VLLLVLYYSLNWIVVAFGIYCVARSVTYIPFDDILLVGSAQAIGYFAALVTLVAPAGL
                     GVRDAAFAWGLKAALPSRSFALGSLIAIAVRGVMTVGELLYVGIVTMLGRREGWSIHT
                     GILHASQEEQEAEEQEDPERPRHPPEIL"
     gene            complement(6619..7662)
                     /locus_tag="VFB52_07415"
     CDS             complement(6619..7662)
                     /locus_tag="VFB52_07415"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZO06201.1"
                     /translation="MLALAPRLGATVVWSLIAVFLVAFAAAPVLLSHDVYSYVDYARL
                     GVVHGLDPYVYPPEAVPSDPAFADVTWPETTSAYGPLFTLATYPLAWLGVGAAVAVLK
                     AAAAAAVGLLAILVARVAAWRGVDPLRAAAFVALNPLVLVHVVGGAHNDGLTMLLAIA
                     AVAAALSARWASAGAALVAATAVKTSAAVIAPFALVASLRRGGPAAARLLAGALVAAL
                     TIVLGSWLAFGWDWTHAFTLAGENQDRTSYMSIPILLSRLTGLDPDPVRAGALALYGL
                     AVVFLLVRTWRGHDWISAAAWASLGLLLATAWLLPWYLIWALPLAAVSRNRPVRFLTL
                     ALTAYQLGARIPL"
BASE COUNT         1127 a         2655 c         2819 g         1202 t
ORIGIN      
        1 agccccgcga tcaggattcg agtcgggtga agagatcgag ccagtcgcgc agcaaccgcg
       61 gcgagaggaa ctggcgccgg gcgtgctcct tgccctcgcg gccgaggctc ttgccgaggc
      121 tcgggttgcc gaggatctcg aggcagcggt cggcgcactc gtcgggcgag ttgacgaggt
      181 agccggagat cccgtcctcg atctgcagcg ggatgccgcc gacgttgccg gcaaccgtcg
      241 gccgcccctt ccagagcgcc tcggtcacgg tcagcccgaa gccctcgcgg atcgacttct
      301 gcaggcagac gtcggactgc gactggaagg cgttgacctc gatcgcgccg acgttgttga
      361 ggttgctgag gatcttgatg tcgggatccg cgtcggcgta ttcgaaggtc ttctggaagt
      421 actcgcggcc ctcgggatcg tcggtcgcca tcgagccgac catcgccagc tgcgcctccg
      481 gcacctgctc ggtgatttcc cggtaggcgt cgatcacgcc gatcgggtcc ttccacgggt
      541 cgaagcgcga gacctgggtg atcagcggcc gctcgacgtc gatcccgaac tggcggcaga
      601 cgaaggcggc gtcctcgggc gagaaggcca tgttcttcgg cgagagcggg tcgatcgccg
      661 gcgggatgat gttgaccccg gcgcgggcgc cgtccatcgc ggcgggcacg taggtcggca
      721 ggtgccagac cgacgcgtca tattcttcga tcatcggcag cagccgctcg atcggcgccg
      781 ggttcggggt cgagaggtcg atgtgacagc gccagatcca gttgttgccc ttctccttgg
      841 cgcctttgtg gaggccgatc ggctgcgggt cgtggacgat cacggtgtcc cattcgcctt
      901 tcagcccctc ggcgttgatc tggttgatct cgtcgaagat ctcccactgc tcgtcgctga
      961 gcgtctcctc ggctccctgc agcgagttgt ggaggagctt ggtcgcgttg aagaactcct
     1021 ccttgccgaa gatcacgtgc cagtgggcgt cgaggccgac gtcccgcatc agcggcacga
     1081 tcgtgtagag gatctcagag acgccgccgc cgaaagcggt ggcgctgacg tggaggacgc
     1141 gcttgccttt gagcggctcg gcaagctcgc gaatctgctc cgtcagctct ttcccgacta
     1201 tgtgggtgta gtcggtgagc gacttctgtc cgagggcgac ttcctgaagc aagtccggag
     1261 ttctacaccc ccgggcggcg acgcgtcgtt tcgaacttgt gtctagcccg ccgggacggc
     1321 gtccggacgg cgtcccaggg tgacctcgac ctcgtctttg tcgccgtcgc gcaggactgt
     1381 cagcttgacc ttgtcgcccg gctggaaggt ggagatcagg cgcgagagat cctccggacc
     1441 gaccaccttg tggccgtcca cttcgaggat cacgtcgccg ccggtgctgt attcgccggc
     1501 ctggaactgg aggttctcgt caccgccgcg caggcccgcc ttctcggccg ggccgccggg
     1561 gacgacggtg gcgagcaggc cgccgaagtt ggtgtcgagg ccgagtttct tcgccagctg
     1621 cgggtagagc gcctggctgg agacgccgat gtaggcgtac tcggcgctgc cgtcggcctc
     1681 gagctgggcg accgagcgtc tgatcgagga gatcggcacg gcgaagccga cgccgtcgtt
     1741 ggcgccggat tcggtctcga tctgctggtt gatcccgatc acgcgggcag cggcgtcgag
     1801 cagggggccg ccggagttgc cggggttgat cgaggcgtcg gtctggatcg cgccctcgat
     1861 ctggaattcg gtcagcgagc gcaccgagcg gtcggtcgcc gagatcacgc cgaccgagag
     1921 cgactgctgc tcgccgaacg ggctgccgat cgccgccacg ggctggccga cctgcagctc
     1981 ggcgtcgtcg cccagtgcca gcgggtgcag cgggacgtcg ccgggctcga cccgcagcag
     2041 ggcgacgtcg acaaagggat cgaagccgac gatctcggcc gcgaccacgt tgcggtcggg
     2101 gaactcgacg aagacttcct cggcggggcg gcggctgccg gtcccttctt cggtgaccac
     2161 gtgggcgttg gtgacgatct cgccgtcggc gttgagcacg aagccggagc cctcggcggc
     2221 gccgctgtcg aagagcgagc ggatcgtgac cacgccgggc gaggccttgc ggtataccgc
     2281 ggccgcgtcg aagccggagg tcgcggtctc gacgatcacc cgggtcggca cctcctcggc
     2341 cgtctccgtg cccgcgctgg tctcgtcgct gcccgaaccg cagccggcga gcaggccgag
     2401 accgagcagc agcagggcgg caagcgccgc gcgcgccctc acgtcgcggc cttggtcgcg
     2461 gcccgcggga cctgggcggc gccttcctgg cgcgccggcg gcaggtccaa cgtgaacgcg
     2521 gtgaagctgt tgcgggagcg gctggagctg atcgtgatcc ggccccgcat ccgctgcgcc
     2581 agctcgctgg cgatcgccag cccgaggccg gagccaccgg ccgagtcggc ggtgtagaaa
     2641 cgctcgaaga tccgattttg gacgcgttgc gggatgccct cgccctcgtc ggcgacggtc
     2701 agctcggcgc ggcggttttc ggaataggtc gtcacggtca ccttcgtgcc ctcaggcgtg
     2761 tgcgtgaggg cgttgtcaag caggatacgg atgatctgtc gcacgcgatc agggtcgccc
     2821 cgggcgatca ccgggcgctc gggtgcgcgc agttccaggc gtgacccgcg cagttcggcg
     2881 cgcgcgccga actctttggt cacctctctc gcgagtccgc tgaggtcgac cgagcgggtc
     2941 tgcatcacca tcgccccggc gtcgagctgg gagaggtcga ggaggtcggc ggtgagcttg
     3001 gtcagccgct cgatctgctg gcgcatctcg cgcacgaact cggcccgctc ctcggggctc
     3061 gggttctcct cgtcgagcag ctcgacgaag ccgccgaggc tgaagatcgg cgtgcgcagc
     3121 tcgtgcgaag cgttggcgat gaactgtttg cgggcgctgt cgagctcgta gagccgtttc
     3181 tgcatctcgt tgaaggtgcg ggccagcggc gccagctggc cgaccgaggc gaccgggatg
     3241 gtcgtttcga agttgccgtc ggcgactttg cgggcggcgt cctggagccg gcgcacgttg
     3301 ctgaccgcga cgcccgcgta atagacgccg aggatcgtcg ccagcgctgc cgcgatcaga
     3361 ccgccgacaa cgatctgcag cttcaccgag tgcaggccgc tggcccctcc ggcgatcgtc
     3421 accgtgtaga ggtagatgac gaggacggtg ccgaggcaga tcccgaccgc cgtgatcgtg
     3481 atccggctgc cgagggtcga gaagcgcagc cgccgcaggg cctactcctt gaagcggtag
     3541 ccgaccccgc gcacggtgaa caggtactcg ggctgcttgg gatcggcctc gagcttctcg
     3601 cgcaggtgcc ggatgtggac gtcgaccgtg cgcgggtcgc ggtactcgga gtcgccccag
     3661 acgtgctcga gcagggtctc gcgggtgagc actcggccgg gcgagcgggc cagggcgccg
     3721 aggatctcga actcgacgta ggtgaccccg atctccccct cgcccagggt gaccatgcgg
     3781 cgatcgaagt cgatcttcag cgccccgctc tcgatcgcgc cctcgtcctc ctgttcgggg
     3841 ctgcccatcc gcgagcgccg cagcgccgct ttcacccggc tgcggaactc gcgcatcgag
     3901 aacggcttgg tgatgtagtc gtcggcgccg acctcgaggc cggcgacctt gtcggtctcg
     3961 ctgcctttcg cggtcagcat gatgatcggc acctggctgc gagagcgcag ctggcggcag
     4021 acttcgaccc cgtcgagacg cggcagcatc aggtcgagga tgaccaggtc gaagcggccg
     4081 tcctcgaagc gctgcagcgc ttcggagccg tcgagcgccg aggtgacgtg atagccgtcc
     4141 ttgcgcagcg gatagctcaa caacgtctga atcgactgct cgtcgtcgac gagaaggatg
     4201 cgggcagacc catttgccac cactcaaggt taatcgcccc gcagacagag cgaaaacgcc
     4261 gctcgttgga tcaagatgtc gatcaggttg gacacatgtg cgattaccca gaactaaggt
     4321 atgcggggaa ggggccgata aaaaaatcaa tgcaagcgca ggagctgaaa tcgctggtcg
     4381 agtcaggcca ctacaaaccg gacccctcgc aggtcgcaat ggcgatgctg cagcgccgtg
     4441 gcgtccggga gctgctcgcg ttctcggtcc cgacggctaa cggagctgat cgaagccgtc
     4501 cggcttcagg tctcccccgc cgggcagcct gatctccact cccgagccgg cggccacttc
     4561 gccgaccggc gtcagcacgg cttcctccgc ttccccgatc gcactcgacg cgtccccgag
     4621 gcgcgcgggt ggcagcgccg cgaacagctc gtagtcctcg ccgccagatg cggcgagctc
     4681 caggggatcc tggccggcgg ccagcgcgac ctcggcgacc cccttggcca gcggcagccc
     4741 cgcggcatcg attcgcagag cgaccccgct ggccgcggcg aggtggccag cgtcggcgcc
     4801 gagcccgtcg ctgaggtcga tcatcgcgct cgccccggcc gcggccaagc cgcggccggc
     4861 gcgcaggcgc ggctgcgggc gctgctggcg ccggcgcagc cgcgcggcgg tctccgcggg
     4921 cacggccgcg gcgagctccg ggcgtttgag cagcagcagg ccggcggccg cgccgccgag
     4981 ctcgccggtc agcaccagca cgtctccggg ggcggccccg gcccgggcaa cgagctgctc
     5041 cggcgcttcg gcgtggccaa cgacggtgac cgcgagggtc agcgccggcg cccgggtgac
     5101 gtcgccgccg gccagcgtgg tgccggtcgc gcgcgccagc gccgccatcc cgtcaagcag
     5161 ccgcaggcac tcgtcctcgt ctacctcggc aggcacaccg aggtagacgt aggcctcgcc
     5221 gggctcggcg cccatcgccg cgaggtcgga gagcgccgtg gccagggcct tgtggccgat
     5281 tgcctccgga tcgctctgct cgcgctgaaa atggacgccg tcgacgatcg cgtcgaccga
     5341 ggttgcggtg gcgccgccgg ggacggtgac cgcggcatcg tcgccgctgc ccaggcgcac
     5401 gcgtgggccc ggcgccggca ggcgctcgcg cagcttcgcc agcagctcga actcgcccat
     5461 ccgttgacta tcccaaaccc gcctcggacg ccgaccgcaa ccggacggct cttaccgcgt
     5521 cattaaggtg ctgatggact tggaggaccg cgaaccactt ctacaccgga tcggcgaggc
     5581 cggcgcgcgg gtcggcagcc gcccaccgcg cagccgccgg gttcgccttg ccctgcagat
     5641 cggcctcgcc gtcctgatct tcggcttcct ggtcctgacc gtgatcgacc agtggtcgga
     5701 gatcaaagag gagggcgtgc acttccacgt cggctggctg gcgccggcgc tggtgatcct
     5761 gcccttcttc tacgtcttca gcgcctacgg ttgggacctg atcctgcgct tcatgggcgt
     5821 gcggatcggg ttcggccgcg cccaggtcgc ctggggccag ccgctgctgg cgcgctacgt
     5881 gcccggcagc gtgctctacg tgctcggccg cgtggtgctc tccgaacggg ccgggatccc
     5941 gcgccggatg acgatcgcca gcatcgtcta cgaacaggcg atctcggcga cctcggcggt
     6001 cgtcgtcgcc tcctacttca tcatcaacca ccccgacctc gaaggcgagc cgtggcgctg
     6061 ggcggtgctg ttgatgatcc ccctggcgat cgtgatcctg cagccgcggg tgttcgggcc
     6121 gctggccaac aaggcgctgc gcgccttcgg ccgcgagccg ctgccggcgg tggtgccgct
     6181 gcacggcgtg ctcctgctcg tcctctatta ctcgctgaac tggatcgtcg tcgccttcgg
     6241 gatctactgc gtggcccgct cggtcaccta catccccttc gacgacatcc tcctggtcgg
     6301 ctccgcccag gcgatcggct acttcgcggc gctggtcacc cttgtcgcac ccgccgggct
     6361 cggcgtccgc gacgccgctt tcgcctgggg cctgaaggcg gcgctgccga gccgcagctt
     6421 cgcgctcggc tcgctgatcg cgatcgcggt tcgcggggtg atgacggtcg gcgaactcct
     6481 ctacgtcggg atcgtcacca tgctcggtcg ccgcgagggc tggagcatcc acaccggcat
     6541 cctgcacgcc tcccaggagg agcaggaagc cgaagagcag gaagaccccg agcgcccccg
     6601 ccacccgccg gaaatcctct aaagcgggat ccgagcccct agctggtagg cggtcagggc
     6661 caaggtgagg aaccggaccg ggcggttgcg ggagaccgcg gcgaggggga gggcccagat
     6721 caggtaccag gggagcagcc aggcggtcgc gagcagcagg ccgagcgagg cccaggcggc
     6781 ggcgctgatc cagtcgtggc cgcgccaggt ccggaccagc aggaagacga cggcgaggcc
     6841 gtagagggcg agcgcgccgg cgcgcaccgg gtcggggtcg agcccagtca gccgggagag
     6901 gaggatcggg atgctcatgt agctggtccg gtcctggttc tcgcccgcca gggtgaaggc
     6961 gtgcgtccag tcccagccga aggcgagcca ggagccgagc acgatcgtga gggcggcgac
     7021 cagggccccg gcgaggaggc gagcggcagc tggtccgccg cggcgcagcg aggcgacgag
     7081 ggcaaacgga gcgatcaccg cggccgaggt cttgaccgcg gtcgcggcga ccagcgcggc
     7141 gccggcgctc gcccagcgcg cgctcagcgc ggccgccacc gcggcgatcg ccagcagcat
     7201 cgtcaggccg tcgttgtggg cgccgccgac gacgtggacg aggaccagcg ggttgagcgc
     7261 gacgaaggcg gcggcccgca gcgggtcaac gccgcgccag gcggcaacgc gggcgacgag
     7321 gatcgccagc agcccgaccg cggccgccgc ggccgccttc agcaccgcca ctgcggcgcc
     7381 gaccccgagc caggcgagcg ggtaggtggc gagggtgaac agcggcccgt aggcactcgt
     7441 cgtttccggc caggtgacgt cggcgaaggc ggggtcggaa ggcaccgctt ccggcgggta
     7501 cacgtatggg tcgaggccgt ggacgacccc gagccgggcg tagtcgacgt agctgtagac
     7561 gtcgtgggag agcagcaccg gcgccgccgc gaaggcgacg aggaagaccg cgatcaggct
     7621 ccagacgacg gttgcgccga gccgcggcgc cagcgccagc accccgaggt aggcgaggaa
     7681 gccggcccag actaggccga aggcgagccc ggggtcgatc gtggcctacc cgttcgagcc
     7741 cgccgattcg cccccgccga cgccgctctc gggcggttcg taagggggcg ggatcggcgc
     7801 cgg
//
LOCUS       DASRIG010000270         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2754205, whole genome shotgun sequence.
ACCESSION   DASRIG010000270 DASRIG010000000
VERSION     DASRIG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754205"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2514
                     /locus_tag="VFB52_07420"
     CDS             <1..2514
                     /locus_tag="VFB52_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06202.1"
                     /translation="ESTDRIALRLTEVRKSFGAVKALKGVSLEVRAGEVHALLGENGA
                     GKSTLMAVAAGSLQPDEGAVEISGERLDAASPSAAQALGLGVVYQHPALADDLTVAEN
                     MVLAMPRKLRPSHGRSGDWVRERLAALGAESIDPSSRVGELTAAEGQLVEIAKALALD
                     PTVLILDEPTAALDAEEVTHLFEQVRAIRARGTAIVYISHRIPEVEEIADRLTVLRDG
                     EARGTFGVEEVDEAAILKLIAGRALTAVFPDKLEAGQAGETVLKVEDLSGEGFDDVSL
                     EVHEGEIVGVAGVAGNGQRELVRALAGLGHASSGTVTVNGAEVNLANPRRAQGEGVFY
                     IPSDRQREGIFPALSVRENAVASALGQFTSFGIVAPGPERAALATEEKNLAIKTPSPE
                     ADIANLSGGNQQKVAFARAMLGRPRVLICDEPTQGVDVGARVEIYRLLRELAADGCAI
                     LVCSSDALELEGLCDRVMIMSRGQTVAALATDAVSEEQITAAAVTATHSRKVKQRETR
                     GSVGWLGRFLRGDSVAAPVLALVIVALAAITGGQNSDFLTSFNIENLLLLSTALILVG
                     LGQLVVVLTAGVDLSVGPLMGLGLVIITAFATDTAGTGGFFVGLLLAVAAGAAVGLVN
                     GGLIQFARINPVIATLATFIGVQGVALLINPVPTGLFSGEAAKTLSTAVGGVPVAFIV
                     VVVLGLGAELALRRSRLGLSLRAVGSDEAAAHRMGIRVGTTKMIAYVICAVFASLAAL
                     MLAVQIGTGDATAGQNYTLQSIAAVVLGGASIYGGRGSFVGTVLGALLLTEMINAITF
                     LELSDAWQYWFPGAIILIAAAIYARASRIRLAAAAADPA"
     gene            2511..>3539
                     /locus_tag="VFB52_07425"
     CDS             2511..>3539
                     /locus_tag="VFB52_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO06203.1"
                     /translation="MSATRVLIKDASVVTLDPDLGDFPRADILVEDGDIVAVGPDLGA
                     SDAETIDASGQIAIPGFANTHLHTWQHALRGVCCDWTVKDYVRCWRIHLGPMYRPEDM
                     YAGNYAGALDALNNGVTLVVDYCHNVVTRDHAREALRGLRDAGVRALFGLAYTPVLDH
                     GFAAEVTQVENEDLPDHDARVRFAHQLAEEEFSSKDGLVTLAIVPQEIEIADWDDVAA
                     EFRSARELDAWISIHANQVLAEQRWEDVRKLHRADLLGPDILFVHCSFSADDELQLIA
                     DSGASVSVQAETEMQMGMGFPITAKLIEHGVAPSLGVDITSNGSGDMFAQMRMALQVG
                     RMLGDQPHY"
BASE COUNT          514 a         1337 c         1182 g          506 t
ORIGIN      
        1 gagtcgactg accgcatagc cctgcgactg actgaggtcc gcaagtcctt cggtgccgtg
       61 aaggcgctga agggtgtgag cctcgaggtt cgcgcggggg aggtgcacgc cctcctcgga
      121 gagaacgggg ctgggaagtc gaccctgatg gccgtcgccg ccggatcgtt gcagcccgac
      181 gaaggggctg tggaaatcag tggcgagcgg ctcgacgccg ccagccccag cgccgcccag
      241 gcgctggggc tgggcgtcgt ctaccagcac ccggccctgg ctgacgacct cacggtcgcc
      301 gagaacatgg tgctggcgat gccgcgcaag ctgcgcccct cgcacggccg cagcggcgac
      361 tgggtccgcg agcgcctcgc cgcgctcggg gccgagagca tcgacccctc cagccgggtc
      421 ggcgaactta ccgccgccga gggtcagctg gtcgagatcg ccaaggcgct ggcgctcgac
      481 ccgaccgtgc tgatcctcga cgagccgacc gcggcgctcg acgccgagga ggtcacccat
      541 cttttcgagc aggtgcgcgc gatccgcgcc cgcggcaccg cgatcgtcta catctcccac
      601 cgcatccccg aggtcgagga aatcgccgac cgcctgaccg tgctgcgcga cggcgaggcg
      661 cgcgggacct tcggcgtcga ggaggtcgac gaggccgcga tcctcaaact gatcgccggc
      721 cgcgccctga ccgcggtctt ccccgacaag ctcgaggccg ggcaggcggg ggagacggtg
      781 ctgaaggtcg aggacctctc cggcgagggc ttcgacgacg tctcgctcga agtccacgag
      841 ggcgagatcg tcggcgtcgc cggggtcgcc ggcaacggcc agcgcgagct ggtccgggcg
      901 ctcgccgggc tcggccacgc gagcagcggc acggtcaccg tcaacggggc cgaggtcaac
      961 ctcgccaacc cgcgcagggc gcagggcgag ggcgtcttct acatcccctc cgatcgccag
     1021 cgcgagggca tcttcccggc cctgagcgtg cgcgagaacg cggtcgccag cgcgctcggc
     1081 cagttcacca gcttcggcat cgtcgccccc gggcccgagc gtgccgcgct cgccaccgag
     1141 gaaaagaacc tggcgatcaa gaccccctcg ccggaggccg acatcgccaa cctctccggc
     1201 ggcaaccagc agaaggtcgc cttcgcccgg gcgatgctgg gccggccgcg ggtgctgatc
     1261 tgcgacgagc cgacccaggg cgtcgacgtc ggcgcccggg tcgagatcta ccgcctgctg
     1321 cgcgagctcg ccgccgacgg ctgcgcgatc ctcgtctgct cctccgacgc gctcgagctc
     1381 gagggcctct gcgaccgggt gatgatcatg tcgcgcgggc agacggtcgc cgcgctcgcc
     1441 accgacgcgg tctccgagga acagatcacc gccgccgcgg tgaccgctac ccacagccgc
     1501 aaagtgaagc agcgcgagac ccgcggctcg gtcggctggc tcggccgctt cctgcgtggc
     1561 gacagcgtcg ccgccccggt gctggcgttg gtgatcgtcg ccctcgccgc gatcaccggc
     1621 ggccagaact ccgacttcct gaccagcttc aacatcgaaa acctgctgct gctctcgacc
     1681 gcgctgatcc tggtcgggct cggccagctg gtcgtcgtcc tgaccgccgg cgtcgacctc
     1741 tcggtcggcc cgctgatggg gttggggctg gtgataatca ccgccttcgc aaccgacacc
     1801 gccggcaccg gcggcttctt cgtcggcctg ctgctcgcgg tcgcggcggg cgccgccgtc
     1861 ggcctcgtca acggcggcct gatccagttc gcccggatca acccggtgat cgcgaccctc
     1921 gccaccttca tcggcgtgca gggagtcgcg ctcttgatca acccggtgcc gaccggcctg
     1981 ttcagcggcg aagcggcgaa gacgctctcg accgcggtcg gcggcgtccc ggtcgccttc
     2041 atcgtcgtcg tcgtgctcgg cctcggcgcc gagctggccc tgcgccgctc gcggctcggc
     2101 ctctccctgc gcgcggtcgg gtccgacgag gccgccgccc accggatggg gatccgggtc
     2161 gggaccacga agatgatcgc ctacgtgatc tgcgccgtct tcgcctcgct cgccgcgctc
     2221 atgctcgcgg tccagatcgg caccggcgat gccaccgcgg gtcagaacta caccctgcag
     2281 agcatcgccg ccgtcgtcct cggcggggcc agcatctacg gcggccgcgg ctccttcgtc
     2341 ggcaccgtgc tcggcgccct gctgctgacc gagatgatca acgcgatcac cttcctcgag
     2401 ctcagcgacg cctggcaata ctggttcccc ggcgcgatca tcctgatcgc cgccgcgatc
     2461 tacgcccgcg ccagccgcat ccgcctcgcc gcggccgccg ccgatccggc atgagcgcca
     2521 cccgggtact gatcaaagac gcctccgtcg tcaccctcga cccggatctc ggcgacttcc
     2581 cgcgcgccga catcctggtc gaggacggcg acatcgtcgc cgtcggtccc gacctcggcg
     2641 ccagcgacgc ggagacgatc gacgccagcg gccagatcgc gatccccggc ttcgccaaca
     2701 cccacctgca cacctggcag cacgcgctgc gcggggtctg ctgcgactgg acggtgaagg
     2761 actacgtgcg ctgctggcgc atccacctcg gcccgatgta ccggcccgag gacatgtacg
     2821 ccggcaacta cgccggcgcg ctcgacgccc tgaacaacgg cgtcaccctg gtggtcgact
     2881 actgccacaa cgtggtcacc cgcgaccacg cccgcgaggc gctgcgcggg ctgcgcgacg
     2941 ccggtgtgcg cgccctcttc gggctcgcct acacgcccgt gctcgaccac ggcttcgccg
     3001 ccgaggtgac ccaggtcgag aacgaagacc tgcccgacca cgacgcccgc gtgcgcttcg
     3061 cccaccaact cgccgaagag gagttcagct ccaaagacgg cctggtgacg ctggcgatcg
     3121 tgccccagga gatcgaaatc gccgactggg acgacgttgc cgccgagttc cgctcggccc
     3181 gcgagctcga cgcctggatc tcgatccacg ccaaccaggt gctcgccgag cagcgctggg
     3241 aagacgtgcg caaactgcac cgcgccgacc tgctcgggcc ggacatcctc ttcgtccact
     3301 gctccttctc ggccgacgac gagctccagc tgatcgccga ctccggcgcc tcggtctcgg
     3361 tccaggcgga gaccgagatg cagatgggca tgggcttccc gatcaccgcc aagctgatcg
     3421 agcatggggt ggcgccgagc ctcggcgtcg acatcacctc gaacggcagc ggcgacatgt
     3481 tcgcccagat gcggatggcg ctccaggtcg ggcggatgct cggcgaccag ccgcactac
//
LOCUS       DASRIG010000271         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1447943, whole genome shotgun sequence.
ACCESSION   DASRIG010000271 DASRIG010000000
VERSION     DASRIG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447943"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFB52_07430"
     CDS             <1..934
                     /locus_tag="VFB52_07430"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HZO06204.1"
                     /translation="ASVGIAMSTAGCDETELLRRADLAMYRAKAHGRDACEFFDPTMD
                     DQAAERLEMVNALRRAVERDELVVHYQPIMDLETGDVRAAEALLRWRRPGRGLVSPLE
                     FIPLAEQTGAIVALGEWVLNAACAEAQRWEREGLSDVPVTVNVSARQLLDPRFEEIVA
                     GALIATGLHPSRLILEVTESSVMQNADVTIAKLDRISSTGVRLALDDFGEGYSSLGQL
                     RQLPIDILKIARPFVRELTDDDHDPALVRGIIELARSLGLRLVAEGIESPEQEAILRA
                     FDCPLGQGFLFAPPLEVGALRALLRERAGAYPNA"
     gene            complement(964..1659)
                     /locus_tag="VFB52_07435"
     CDS             complement(964..1659)
                     /locus_tag="VFB52_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO06205.1"
                     /translation="MAHLNLTERDGVSVLSADRPPANAMDVTLLDELVGAVEGLAAEP
                     PAALVLAGRPGFFSAGLDLKAVPGYGPPEQRRLVDGINRMAIGVYGLPCPVVCAITGH
                     AIAGGFVLAVCGDHRVASTEGRYGLTEIKVGVPYPQGAIGVVRAELTAPAARLLVLGS
                     RLVDAAECVRLGAFDEALPPDQVVDRSLEVARELAELPAEVYARTKAELRGAILTDLR
                     AAAEADPLLASWV"
     gene            complement(1666..1739)
                     /locus_tag="VFB52_07440"
     tRNA            complement(1666..1739)
                     /locus_tag="VFB52_07440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1703..1705),aa:Arg,seq:cct)
     gene            2007..>2233
                     /locus_tag="VFB52_07445"
     CDS             2007..>2233
                     /locus_tag="VFB52_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06206.1"
                     /translation="MSTGAGASDLLHAEDTGLDQMVRGEQAVAARSPLQLFWRRFRRD
                     KVAMTALAFIVVLILVAIFAGPITKLIGAAPP"
BASE COUNT          327 a          775 c          822 g          309 t
ORIGIN      
        1 cgcgagcgtc gggatcgcga tgtcgaccgc gggatgcgac gaaaccgagc tgctgcgccg
       61 ggccgacctg gcgatgtacc gcgccaaggc ccacggccgc gacgcttgcg agttcttcga
      121 cccgacgatg gacgaccagg ccgccgagcg gctcgagatg gtgaacgcat tgcgccgggc
      181 ggtcgagcgc gacgagctgg tcgtgcacta ccagccgatc atggacctcg agaccggcga
      241 cgtgcgcgcg gcggaggcgc tgctgcgctg gcgccggcct ggccgcgggc tcgtgtcgcc
      301 gctcgagttc atccccctgg cggagcagac cggcgcgatc gtcgcgctcg gcgaatgggt
      361 gctgaacgcg gcctgtgccg aggcccagcg ctgggagcgc gagggcctgt ccgacgtgcc
      421 ggtgaccgtg aacgtgtcgg cccgccagct gctcgatccg cgcttcgagg agatcgtcgc
      481 cggcgcgctc atcgcgaccg ggctccaccc cagcaggctg atcctcgagg tgaccgagtc
      541 gtcggtgatg cagaacgcgg acgtcacgat cgccaagctg gaccggatct cgtcgaccgg
      601 cgtgcgcctc gcgctcgacg acttcgggga ggggtactcc tcgctcggcc agctgcgcca
      661 gctcccgatc gacatcctga agatcgcgcg tccgttcgtc cgcgagctga ccgacgacga
      721 ccacgacccg gcgctggtgc ggggcatcat cgagctggcg cgcagcctcg ggctgcggct
      781 ggtggccgag ggcatcgagt cgccggagca ggaggcgatc ctgcgggcgt tcgactgccc
      841 gctcgggcag ggcttcctgt tcgccccgcc gctcgaggtg ggggcgctgc gagcgctgct
      901 gcgcgagcgc gccggcgcct accccaacgc ctgacctcag gcgtggtcgg gtgaagccgg
      961 cgcctagacc cagctggcga gcagcggatc ggcctcggcc gcagcgcgca ggtcggtgag
     1021 gatcgcgccg cgcagctcgg ccttcgtgcg ggcgtagacc tcggcgggca gctcggccag
     1081 ctcccgggcg acctccaggg agcggtcgac tacctgatcg ggcgggagcg cctcgtcgaa
     1141 agcgcccaac cgcacgcact cggcggcgtc gaccaggcgg ctgccgagga cgagcaggcg
     1201 ggccgccggg gcggtgagct cggcgcgcac cacgccgatc gcgccctgcg gatagggcac
     1261 gccgaccttg atctcggtca ggccgtagcg gccctcggtc gaggcgacgc ggtgatcgcc
     1321 gcacacggcc agcacgaacc cgccggcgat cgcatgaccg gtgatggcgc agacgaccgg
     1381 gcagggcaac ccgtacacgc cgatcgccat ccggttgatc ccatcgacga ggcgacgctg
     1441 ctccggcggg ccgtagccgg gcaccgcctt gaggtcgagg ccggcggaga agaagccggg
     1501 gcgaccggcg agcaccagcg ccgccggcgg ctccgcggcg agtccctcga cggcgccgac
     1561 cagctcgtcg agcagggtca cgtccatggc gttggccggc ggccggtcgg cgctgaggac
     1621 cgacaccccg tcgcgctcgg tgaggttcag gtgagccacg gcgagcgccc cgagctggat
     1681 tcgaaccagc ggcctactcc ttaggaggga gtcgctctat ccggctgagc tatcggggct
     1741 cggcgatcac gcatcttaat ccgcaaccaa ggggatgccg cgaccgcgga gctatattgg
     1801 ccgccagttg gacccgccga cctcgggcag gcagggaaca cgcatgtgac ctgcgtaccc
     1861 gccggttggc gtgtcggatg ggttgggccg aagactttgt aggttgacgg ccttaaaggg
     1921 gaatagtgcc gatgcgcccg cccctgcgcg cgcatcgggg agaggcatca actgaacatc
     1981 gcagtatcca gccgagaggc gctcgtatga gcacgggcgc cggcgcgagc gacctcctgc
     2041 acgcggagga cactggcctc gatcagatgg tccggggtga gcaggcggta gccgcccgct
     2101 cgccgctcca gctgttctgg cgtcgcttcc gccgtgacaa ggtcgcgatg acggcgctcg
     2161 ccttcatcgt ggtcctgatc ctggtcgcga tcttcgccgg cccgatcacc aagctcatag
     2221 gcgctgcgcc gcc
//
LOCUS       DASRIG010000272         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1189434, whole genome shotgun sequence.
ACCESSION   DASRIG010000272 DASRIG010000000
VERSION     DASRIG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189434"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VFB52_07450"
     CDS             <1..1756
                     /locus_tag="VFB52_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO06207.1"
                     /translation="AEDAFWDPPSFPPFKIVENNQIVPDMEALYRRCSRTPENLSLDL
                     RATIAGNNAARSRILGLVEKYGKAVVKGVMRRALDASQRAMEQALDTIPDGVYSERLI
                     QEVSRNGDRGTYPVQTTVRKQGRELIWDNVGTHDQVGAINVGFAAWRGAILGSINVLM
                     LPGQMGCIGGAARLCKFDPEPGTITAPDWGAAVSPAGVYATETAISLGNALISKMMLA
                     SSDEQMRANAVTPDGAQWPCHFVAGDNQRGDYYVGGMGDNMLGGGSASFGRDGEFANG
                     HFWIPEGRGPNVEAYEADWPILYLFRREHPDSGGAGRRRAGNGGEIAYVPHKGESFLG
                     LYCNEGIPKTPGLLGGDPGSIMVTRVVQGSDLRKRFAAGELPQDLDALEGTELAIGGK
                     GDGFPVTDDDVLYWNWLGPGGYADPLTRDPDEVAADLEAGAVSPAGVDHFYGVRFDDE
                     RKVDRERTESYRERKLLERLHECGAEREQLAPIKAVPAGAETIGDVYLVDREGGVLRC
                     HRCATDLGPLEANAKERMAVRERPLVEIKPGHPDPARFVDDEVVWRDYCCPGCGVRLA
                     TETAYPGEAPFQELSLDP"
     gene            1767..3428
                     /locus_tag="VFB52_07455"
     CDS             1767..3428
                     /locus_tag="VFB52_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06208.1"
                     /translation="MPLADPGLSRWVPTAGDEPPLRELTVGGLLDQAVQKWPDHEAVV
                     FSSYADMGIEARWSFAELRERARRAGRALIASGIEPGERFGIWATNIPEWLELQFGAA
                     YTGAVVVPMNPLYRQSEVEFVLAKATASACFVIPENRGASLWEMTVAASAGLDHVRTL
                     VPIGAAQDGAPSWEEWLASGDSVDEALLERRAAAVRPADTSQIQFTSGTTGFPKGAEL
                     SHFGIVNDARLFAHRANLRQHGRHSNPMPYFHCGGCVMSTLGSVATGSAQMPAVTFDA
                     ARIARTIEEEGCTSVSLVPTMMIGLEEQVAKDGRDLGSLEIVVTGGSPVPPEVMRRWI
                     DRYGVGINNTYGMTEASPVICATLPDDSVERQTTTVGPPLPHLEVDVVEPGGTAPVPV
                     GVEGELRTRSAMVMAGYWDNPEATAAAIGADGFLRSGDLAKMDAEGYVSITGRAKEMI
                     IRGGENIYPAEVENAIRELEAVADVAVIGVPDERYGEVCAAYVRLVDGATLSYAELKE
                     QLTGRVARYKCPAHLRVLDEFPLTPSGKVQKFKLKEHFEASLDGG"
     gene            3418..>3941
                     /locus_tag="VFB52_07460"
     CDS             3418..>3941
                     /locus_tag="VFB52_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06209.1"
                     /translation="MAAERWFSERELAAMSRPTMDRAIEAIKAGDTELALELCEGMKY
                     EWRFLHDVMAESMLGLVDYIQQKLGDDGVGECWEVALRRGWKRDTGKVLDRDRREIVE
                     ALAASWRAHSGSATGPSPGAFTISEDEEKFTFAMNPCGSGQRLWRNGAYEGEGAYGVT
                     EEAHDWSYGREGFPL"
BASE COUNT          601 a         1405 c         1412 g          523 t
ORIGIN      
        1 cgccgaggac gccttctggg acccgcccag cttcccgccc ttcaagatcg tcgagaacaa
       61 ccagatcgtc cccgacatgg aggcgctcta ccggcgctgc tcgcgcaccc cggagaacct
      121 ctcgctcgac ctgcgggcga cgatcgccgg caacaacgcc gcccgctcgc gcatcctcgg
      181 cctggtcgag aagtacggca aggcggtggt caagggcgtg atgcggcggg cgctcgacgc
      241 cagccagcgg gcgatggaac aggccctcga cacgatcccc gacggggtct actccgagcg
      301 cctgatccag gaggtctcgc gcaacggcga ccgcggcacc tacccggtcc agaccacggt
      361 ccgcaagcag ggccgcgagc tgatctggga caacgtcggc acccacgacc aggtcggcgc
      421 gatcaacgtc ggcttcgccg cctggcgcgg cgcgatcctc ggctcgatca acgtgctgat
      481 gctgccgggg cagatgggct gcatcggcgg cgccgcccgg ctctgcaagt tcgaccccga
      541 accggggacg atcaccgcgc ccgactgggg cgccgcggtc agcccggccg gcgtctacgc
      601 gaccgagacc gcgatctcgc tcggcaacgc gctgatctcg aagatgatgc tcgcctccag
      661 cgacgagcag atgcgcgcca acgcggtcac ccccgacggt gcccagtggc cctgccactt
      721 cgtcgccggc gacaaccagc gcggcgacta ctacgtcggc gggatgggcg acaacatgct
      781 cggcggcggc tcggccagct tcgggcgcga cggcgaattc gccaacggcc acttctggat
      841 cccggagggg cgaggcccga acgtcgaggc ctacgaggcc gactggccga tcctctacct
      901 gttccgccgc gagcaccccg actccggcgg cgccggccgg cgccgcgccg gcaacggcgg
      961 tgagatcgcc tacgtcccgc acaagggcga gtccttcctc ggcctctact gcaacgaggg
     1021 cattccgaag acgccgggcc tgctcggcgg cgatcccggc tcgatcatgg tcacccgggt
     1081 ggtgcagggc tccgatctgc gcaagcgctt cgccgccggt gagctgcccc aggacctcga
     1141 cgcgctcgag gggaccgagc tggcgatcgg cggcaagggc gacggcttcc cggtcaccga
     1201 cgacgacgtg ctctactgga actggcttgg cccgggcggc tacgccgacc cgctgacccg
     1261 cgacccggac gaggtcgccg ccgacctcga ggccggcgcg gtcagccccg cgggggtcga
     1321 ccacttctac ggcgtccgct tcgacgacga gcgcaaggtc gaccgcgagc ggaccgagag
     1381 ctaccgcgag cgcaagctgc tcgaacggct gcacgagtgc ggcgccgagc gcgagcagct
     1441 ggcgccgatc aaagcggtgc cggcgggtgc cgagacgatc ggcgacgtct acctggtcga
     1501 ccgcgagggc ggcgtgctgc gctgccaccg ctgcgccacc gacctcggcc cgctcgaggc
     1561 caacgcgaag gagcggatgg cggtgcgcga gcggccgttg gtcgagatca agcccggcca
     1621 ccccgacccg gcccgcttcg tcgacgacga ggtcgtctgg cgcgactact gctgccccgg
     1681 ctgcggcgtg cgcctggcaa ccgaaaccgc ttaccccggc gaggctccct tccaggagct
     1741 gagcctcgat ccgtgagggt cggctgatgc cgctcgccga tcccggcctc tcgcgctggg
     1801 tcccgaccgc cggcgacgag ccgccgctgc gcgagctgac cgtcggcggc ctgctcgacc
     1861 aggcggtcca aaaatggccc gaccacgaag ccgtggtctt ctcctcctac gcggacatgg
     1921 gaatcgaggc gcgctggagc ttcgccgagt tgcgcgagcg ggcccgccgg gccggccggg
     1981 cgctgatcgc ctccgggatc gagccgggtg agcgcttcgg gatctgggcg accaacatcc
     2041 ccgagtggct ggagctgcag ttcggcgccg cctataccgg cgccgtggtc gtgccgatga
     2101 acccgctcta ccgccagagc gaggtcgagt ttgtgctcgc caaggcgacc gcctcggcct
     2161 gcttcgtgat ccccgagaac cgcggcgcct cgctctggga gatgaccgtt gccgcgagcg
     2221 ccggcctcga ccacgtgcgc accctggtcc cgatcggcgc ggcccaggat ggcgccccct
     2281 cctgggagga gtggctggcc agcggcgatt cggttgacga ggctctgctg gagcgccgcg
     2341 ccgccgccgt gcgcccggcc gacacctcgc agatccagtt cacctccggg accaccggct
     2401 tcccgaaggg ggccgagctc agccacttcg ggatcgtcaa cgacgcccgg ctcttcgccc
     2461 accgcgcgaa cctgcgccag cacgggcgcc actccaaccc gatgccctac ttccactgcg
     2521 gcggctgcgt gatgtcgacg ctcggcagcg tcgccaccgg ctcggcgcag atgcccgcgg
     2581 ttacgttcga cgctgcgcgg atcgcgcgca cgatcgagga ggagggctgc acatcggtct
     2641 cgctcgtccc gacgatgatg atcgggctcg aagagcaggt cgccaaggac ggccgcgacc
     2701 tcggctccct ggagatcgtc gtcaccggcg gctcgccggt gccgccggaa gtgatgcggc
     2761 gctggatcga ccgctacggg gtcgggatca acaacaccta cgggatgacc gaggcctcgc
     2821 cggtgatctg cgcgaccctg cccgacgact cggtcgagcg gcagacgacc acggtcgggc
     2881 cgccgctgcc gcacctcgag gtcgacgtgg tcgagccggg cggaaccgcg ccggtcccgg
     2941 tcggggtcga gggggagctg cgcacgcgca gcgcgatggt gatggccggc tactgggaca
     3001 accccgaggc gaccgcggcg gcgatcggcg cggatggttt cctgcgctcc ggcgacctgg
     3061 cgaagatgga cgccgagggc tacgtctcga tcaccgggcg ggccaaggag atgatcatcc
     3121 gcggcggcga gaacatctac ccggccgagg tcgagaacgc gatccgcgag ctcgaggcgg
     3181 tcgccgacgt ggcggtgatc ggggttcccg acgagcgcta cggcgaggtc tgcgccgcct
     3241 acgtgcggct cgtcgacggc gcgacgctga gctacgcgga gctgaaagag cagctgaccg
     3301 gccgggtcgc ccgctacaag tgccccgccc acctgcgggt gctcgacgag ttcccgctca
     3361 ctccctcggg caaggtgcag aagttcaagc tgaaagagca cttcgaggcg agcctcgatg
     3421 gcggctgagc gctggttcag cgagcgcgag ctggccgcga tgtcgcggcc gacgatggac
     3481 cgcgcgatcg aggcgatcaa ggccggcgac accgagctgg cgctggagct gtgcgagggg
     3541 atgaagtacg agtggcgctt cctgcacgac gtgatggctg agtcgatgct gggcctggtc
     3601 gactacatcc agcagaagct cggcgacgat ggcgtgggcg agtgctggga ggtggcgctg
     3661 cggcgcggct ggaagcgcga caccggaaag gtgctcgacc gcgaccgccg cgagatcgtc
     3721 gaggcgctgg cggcgagctg gcgggcgcac tcgggctcgg ccaccggtcc cagcccgggc
     3781 gccttcacga tcagcgagga cgaggagaag ttcaccttcg cgatgaaccc ctgcggctcc
     3841 ggccagcgac tttggcgcaa cggggcgtac gagggcgagg gcgcctacgg cgtcaccgaa
     3901 gaggcgcacg actggtccta cggccgcgag ggcttcccgc t
//
LOCUS       DASRIG010000273        17732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3527346, whole genome shotgun sequence.
ACCESSION   DASRIG010000273 DASRIG010000000
VERSION     DASRIG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527346"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFB52_07465"
     CDS             <1..840
                     /locus_tag="VFB52_07465"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO06210.1"
                     /translation="GLLECAAGVQEVADSLMEDKIRSMTVARGHDPRDFVLYAFGGGG
                     AVHAGLFSRGLGISKVVVPVGDLASVWSAYGIGLEGYGRTYELPAYMRAPFELARIAA
                     GFEQLDKLAREDAARSGVEWESLLSARSADLKYALQVYDVEAEVPDGRLGEGAPQQIV
                     DNFERNYARRFGEGVGYPEGGVDMTALRLHVEPANSLRAGAASDAGAGGSAEPAGSRD
                     VYWPENGKVEATPVYTGAELPEGTRLTGPALLDFPDTTVVVRPGLDLVVEPGGNLTIE
                     MEA"
     gene            843..3155
                     /locus_tag="VFB52_07470"
     CDS             843..3155
                     /locus_tag="VFB52_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO06211.1"
                     /translation="METVARTLENERRRRAESFVDGYLPPDELVIDPSLQFHNEVDTD
                     LDPVSYEVLRSKLWNVNWDHQETIRRVSGSQVVVYGYDFNTTIQTEDGAGVCFGPGNL
                     FFGGCADVVVKWTLEHRSMNVGINEGDIFLQDDPWIGTNHQMDAAVYAPVFWEGKLFA
                     WVYNVIHQRELGGVEPGGFTQQAHDVYAEPTFFPPMKLVDAGKVREDVVDAWVRRSRL
                     AGLMTLELRSQIAGVEVARARVLDLVERYGAARVKGTMRKMIDTTASVVGDRLASLPD
                     GEWTDTRYVSGAVVGDLTPYKYSLTVRKEGDRLTYSNKGTDPSVGSFNITAGVLRACI
                     TNSLLTFLCYDQYLCAAGLLEQVDFEFERGTITAAHHPAAVSTSLGLVVALVQAQYLN
                     AKMLSTNEETAHHTFGVSGCATLIYNHTFGVDQYGNPTANFPLDGIVGGLGAFPWRDG
                     LDHGGTMSSTMNPVGSVESMEREVPMLYLYRREATDSGGHGRWRGGACIVSAMVGHRS
                     PEHYISSGGLAQSVTQGMGLLGGWPATGGTMWRAENTEIRKWLDGGRVPGTPDELREM
                     APDGDLAVPKVFNNKLFEDDVFEVIPNPGAGYGDPMLREAETVAAEVVEGRLTAAEAR
                     AIYGVDVNEAGELAAGHEEFRRERRQARLAEAKPPLEATEGKIAEAEGLALASVAFGK
                     GDGGEQLLGCRECGHALAERQGNYRLGAARLELAMTELGATFIDPQEQVGHELIFRSY
                     LCPGCGTALDGEVCKPDDPPVWDVRLAPGG"
     gene            complement(3391..3735)
                     /locus_tag="VFB52_07475"
     CDS             complement(3391..3735)
                     /locus_tag="VFB52_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06212.1"
                     /translation="MSVDLFAGMPVSDFATAKAWYETLLGGEPAFMPHETEAVWELAE
                     HRYLFIVEDEKRAGTAIPTVFVAEFDALLAAIAERGLEPAEVETYSNGVRKAIFRDPD
                     GNELGYGGGPAD"
     gene            3863..4249
                     /locus_tag="VFB52_07480"
     CDS             3863..4249
                     /locus_tag="VFB52_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06213.1"
                     /translation="MTRKTKFRRSITALAVTALALAGLGGSAAGAAAATPTKVAIFAE
                     SGGFYGFVKSASPECKVERTVAVYKQLGAKQRPATDERVAMDTTGEDGEWNIGNPGLR
                     SGRYYARATVKPGCKAANSITLRAQQ"
     gene            4474..7647
                     /locus_tag="VFB52_07485"
     CDS             4474..7647
                     /locus_tag="VFB52_07485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZO06214.1"
                     /translation="MLGVAPASALAGSISGSLTDAETGAPVTDSLVCAYGVNGGGYCV
                     GVPANGEYAIGSLPADEYRVSFRAYPGNNYAPQYWQEADIWDDATLIALGADEDRTGI
                     DAALEPGAMLSGTVVGEGEIGPLEGVQVCTTDLVRPGGLIECVETDADGEYQLTGLRS
                     GSYRVTFSTPWSSVEYATRYYDEKAFEDEADELQLTAGESATADATMSPAGAIEGTVT
                     GEGEPLEWAWVCAYTLAGDKLGCAPTDEDGHYRYGGLPAGSYVLEVEGYGFMPEFSGD
                     ASTFEEATPVTVLAGETTPFDADLSAPPGIEGTVTAAASGEPLESVRVCAVPDFESTT
                     SCVYTWEDGSYKIENLGTDSYTVSFEADGYAIQYYDGVLAESEATKVPVSDGMVSGID
                     AELVPGGLIKGHVTLASSGANLGSVKACALRADGSEAGCDSTATSSGIFEIEDIPVGE
                     YKVRFTRSSYVTQYFNGKATFEAADPVTVAAGETTEGVDAAMVKLDKPKNTALPALTG
                     VGKVGETLACSDGTWSGNPSKYEYYWYRNGFEIQGALLSTYKLVSGDAGKTISCAVET
                     WNSAGWSDQALAPTSIQVAAIRQLTVSKAGTGSGSVTSSPSGIDCGSTCSTSGNQGET
                     ITLTAAPAQHSQLSGWAGCTTSSGTECTVTLGGANASVTATFAPITHTVSVAVTGGGS
                     VSAASGAITGCTPSGGACSGSYDETSEVVLTATPNAHQSFTGWTGCTTAAGTECKVTV
                     DGAENVTAGFAPILHELEVAKTGGGSGSVTSNPAGIDCGSTCADEFEEGSTITLSATP
                     AQHSEFTGWSGGGCSGTATCAVTLGADIEVSAGFAPITHGVSVAVTGGGSVSAASGAI
                     AGCTASAGTCNGSYDEGAEVVLTATPAAHQSFSGWSGCTTATGAECKVTIDGAESVTA
                     TFAQITHELSVSKAGTGSGSVTSTPVGIDCGSLCATSFDEGEEVGLTATPAADSEFTG
                     WSGGGCTGTGTCTVSVEADTAVTATFAKKATEGGTVIPPPTPPTPGPAPKPKPKPLKC
                     KKGFKKKKVKGKQRCVKVKKKQSGKRA"
     gene            7693..9321
                     /locus_tag="VFB52_07490"
     CDS             7693..9321
                     /locus_tag="VFB52_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO06215.1"
                     /translation="MDYGHELEFGVFITPANERPEGVVRLAELAEELGLDLVSFQDHP
                     YQSRFLDTWTLLSYLAARTNRVRLAPNVLNLPLRQPAVIARAAASLDLLSDGRVELGL
                     GAGAFWDAIEAMGGRRLSGGEAVDALEEAIAVIRGIWDTGERGGVRVEGEHYKVRGAA
                     RGPAPAHEIGLWIGAYKPRMLRLTGRLGDGWLPSVPYMEPADVAAGNAAIDAAAEQAG
                     RDPRAVRRVLNVSGAYSDGTPGPLSGSAEQQAEELARLALEEGVSVFVATGDDPAEVD
                     RFATEVAPATRELVAAERERRVEEGPSATVTLAGSGADAPSANGASATGAPPQSDEAG
                     APTSEYERLGVTPTPDDGNRLAAPQWDESTRPHRTPSGPEVSYTDAGRHAGQHLIDVH
                     DHLRNELTQLREVVAQVRDGALGAGEARDALHAMALRQNDWTLGAFCSRYCRVVTQHH
                     TIEDQSVFPHLRGSDSELEPVIDRLTEEHLTIHDAIDAVDRALVHHLEHPDDFDRLQT
                     AIDVLTDTLLSHLSYEEHELVEPLARLGFQPSRL"
     gene            9565..9972
                     /locus_tag="VFB52_07495"
     CDS             9565..9972
                     /locus_tag="VFB52_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06216.1"
                     /translation="MSFPETPSHVYVPVPVDRVDDVFRLLLGLKQRGGEDVPEHLLQR
                     AYSESDTQFQALLRYLAERPDEAIGSVQIANELGLANGTASLAGMLGAFARRAKNRYD
                     GFWPFERIYSPGDEGSRLIMTAEIATAIKRLAA"
     gene            complement(9953..10354)
                     /locus_tag="VFB52_07500"
     CDS             complement(9953..10354)
                     /locus_tag="VFB52_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HZO06217.1"
                     /translation="MKGNRKRDTRPELALRSALHRLGLRYRVDTRPLRELRCKADVVF
                     RRQRVAVFVDGCFWHSCPEHGTSPKTNSSYWSAKIARNVERDRRNDAELNAAGWTVIR
                     VWEHEDPAARAREIAAMLAATDADRATRRAA"
     gene            10418..11548
                     /locus_tag="VFB52_07505"
     CDS             10418..11548
                     /locus_tag="VFB52_07505"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZO06218.1"
                     /translation="MDAISLFSGAGGLDLGCEAAGFETVGVVEVEEVARQTMLANRED
                     FFPRLREEAVLTDILEANPGDLLDRAGLQPGEVSLLHGGPPCTPFSKSGYWLAYKRAG
                     EDPRASLLDEYVRMLRGIKPKAFLMENVYGLAYRNQNRAVFERFLSAARRAGYAVDHR
                     ILLAADLGVPQLRQRLFCVGIRRDLLSGSSRGWEMEWPASTHSGPHETRTGWDEGLLP
                     HKTTGEALESLPERLNPEEPEEIVEGTFADSLRAVPPGDNYLFWTAERGHKEPRFKWR
                     SRYWSFLLKLHPDKPSPTIQGQPGPWVGPFHWEGRRLRVAEVKRLMTFPDEYTVLGAR
                     REQQLQLGNAVPPDLGRAVARAIAEELSRLDSASAPGLALAA"
     gene            11579..12655
                     /locus_tag="VFB52_07510"
     CDS             11579..12655
                     /locus_tag="VFB52_07510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease, SacI family"
                     /protein_id="HZO06219.1"
                     /translation="MGIRLEDEALQLTLEQALSLARSGKPLPTIWLDRVERLGELGVK
                     TYIAALGGALLAKATNPEVDSLLQDENAGNGGYSLRRAAEFLALHNEGRYHLGAQGRW
                     PLNNRPFLGGPSRIDEFKKISGRARPSYELFLDTLRDLRRRDEPEALEALAAFLRVRM
                     QVQEEARTAAREARGVESDLAIADLVRVIDLFVHRDSEGGKRAQALTAAVLDCAFDEV
                     DMGPINGPRAGDVRVLSNDRVIVAAEAKQLEAEDADATKLSSDAAELGADLALLVVLF
                     KRHRSLDREALRQAALREDGTLLVICESVIELVTSVAVYSATPAAHIETELPGAYALR
                     MREIEVSPAALEEWEHQIKARTGR"
     gene            complement(12652..13659)
                     /locus_tag="VFB52_07515"
     CDS             complement(12652..13659)
                     /locus_tag="VFB52_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO06220.1"
                     /translation="MAQAAITDKPPADTVKELAALSARLDEKVPRRTRNNLLVATWNI
                     RKFGGLTDKWHSVKADRPKRDLHALACITEIVSRFDVVAIQEVQTEIRALRHMLKLLG
                     SDFTVILTDAVEDHKGNNERLAFVFNKKRLEVSGLACELVVPLNWTKKRKPKAGIDRV
                     ALKRQFARTPYAVAFRRGNSTFILVTLHVFWGTGKQGIRARTAELTAIATWLRSWAED
                     TNSWDQNLICLGDFNIDKKDGKLWKAFTSTGLTTPSPIDQPRIVEDKPLKGFYDQISW
                     FVDENDGRKSLISLDYTGKANVFDWSTTVLAKSGMSKTDKSHRISDHYPLWVEFALPK
                     S"
     gene            complement(13732..15426)
                     /locus_tag="VFB52_07520"
     CDS             complement(13732..15426)
                     /locus_tag="VFB52_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO06221.1"
                     /translation="MPEAQAEIKVVRSENFPDLSDTQIYPRLSEAKFAWLRERGAVEK
                     RFEPGEVLYEHAVRDAPFFVIESGLVEFVDRKPGKNVHIAQADAGTFIGDIAIFTGEP
                     TISACVAVEPTVTLAFDRPGLRDLVARWPEFGEHIFGTLLARREWHEAGGHGVMRLIA
                     PAGSRRAFEVRDLLERNLLPVRWYDVDNDPESAQMLDWLGIPAEETPVLVHARTVLRN
                     PSPAQAARQLGLRAEVDGERFDLVVLGAGPAGLAASVYGGSEGLRTFVAESWAPGGQA
                     GTSTRIENYLGFPSGIGGRELTTKATLQARRFDAVISSFHRAVELADGPEGLIRVDLD
                     DGQHVLARALVMATGARWRELEAEGMSRFRGAGVYHAAMSTDAERSRDKDVIVVGGGN
                     SAGQAAVHLANRARSVRVVVRGKALKSTMSHYLIDRIKTHPRIEVMTETEITALQGKS
                     TVESVTLRDNRDGTTSEVDTTAVYVMIGADPCTEACDGILAVDEAGYLLCGEGAEACP
                     QHLCWPLSDREPHLLETVRPGVFAAGDVRAGAAKRVAGAVGDGALTVRFAYDVLAG"
     gene            complement(15457..>17732)
                     /locus_tag="VFB52_07525"
     CDS             complement(15457..>17732)
                     /locus_tag="VFB52_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06222.1"
                     /translation="LLLVLDDLHWADRATLHLLRHTIRSSQEGSLLIVGAYRDAEIGP
                     EHPLADLLADLRRDRLFDRVSLDGLDEGGVNELIALHAGHAAPAALVGTVHEHTEGNP
                     FFVEEVMRHLIETGVLFERGGRWTSALTADEIGVPEGVRDVLGGRLGRLSENCRAALA
                     AAAVLGREFRFEALLAMEVADEEALIAALEEAVEAQLVVEAEGAAATHGFTHALVREA
                     LYSELSGPRRQRMHARAAAALEATAGEPPIAALAVQYRLAGAAGDPEKAVAYSVRAGG
                     EAAEVSAWEEAAAHWEGALAVMAAVAGRERERAELDLLLGDLLGVAGDVGGQIARLED
                     ALALFEGLGDVERTAQAHSRLGSAYSLIDSIYADHMDLNVAFRHFDAARAVFEQGPPR
                     RALGHLEGGVAAALTYGVQTERGIEAGARSMEIGERTGDEVLWAKGAESGGWHLIAAG
                     QLAEGFAQMERALEVVDRERRALLAWMALNIRGQMSWGLCSPDEAQGFFERHIALPYV
                     GETSFRQETADGVGRCHFSRGELGPARALLSDARPAWMTHALKPLLDLWDGRWDEVAA
                     LATRTLETSRRNGNRWDEWSSQHLAAHVHHLRGELAPAAEYLERAAAIVGPGGARYFE
                     LWVRIEQARVSADAGDLAVARTHLDRCLEIVGNGEDWRGRGGQVALAEAVVLAGEDRP
                     DQAEAAFARALETFGRYRLPAEQADALHQWGRALAGAGERGAATEKLDRAAAIYREHG
                     AGERWLALVEADREPLL"
BASE COUNT         2748 a         6207 c         6179 g         2598 t
ORIGIN      
        1 ggcctgctcg agtgcgcggc cggcgtccag gaggtcgccg actcgctgat ggaggacaag
       61 atccgcagca tgaccgtggc ccgcggccac gacccgcgcg acttcgtcct ctacgccttc
      121 ggcggcggcg gcgccgtcca cgccggcctt ttctcgcgcg ggctggggat ctccaaggtg
      181 gtggtcccgg tcggcgacct cgcctcggtc tggtcggcct acgggatcgg gctggagggc
      241 tacggccgca cctacgagct gcccgcctac atgcgcgccc cgttcgagct ggcgaggatc
      301 gccgcgggct tcgagcagct cgacaagctg gcccgcgaag acgcggcccg ctccggggtc
      361 gagtgggaat cgctgctcag cgcgcgcagc gccgacctca agtacgcgct gcaggtctac
      421 gacgtcgagg ccgaggtccc cgacgggcgg ctcggcgagg gcgccccgca gcagatcgtc
      481 gacaacttcg aacgcaacta cgcgcgccgc ttcggcgagg gcgtcggcta cccggagggc
      541 ggcgtcgaca tgaccgcgct gcgcctgcac gtcgagccgg ccaactcgct gcgcgcgggc
      601 gccgcctccg acgccggcgc gggcgggagc gccgaacccg ccggcagccg cgacgtctac
      661 tggcccgaga acggcaaggt cgaggcgacg ccggtctaca ccggcgccga gctgcccgag
      721 ggcacgcgcc tgaccgggcc ggcgctgctc gacttccccg acaccaccgt cgtcgtgcgg
      781 ccgggccttg acctggtcgt cgagccgggc ggaaacctga cgatcgagat ggaggcctga
      841 acatggagac cgtcgcccgc accctcgaga acgagcgtcg ccgccgcgcc gaaagcttcg
      901 tcgacggcta cctgccgccc gacgagctgg tcatcgaccc ctcgctgcag ttccacaacg
      961 aggtcgacac cgacctcgac ccggtctcct acgaggtcct gcgctcgaag ctctggaacg
     1021 tcaactggga ccaccaggaa acgatccgcc gcgtctccgg cagccaggtc gtcgtctacg
     1081 gctacgactt caacacgacg atccagaccg aggacggggc cggcgtctgc ttcggccccg
     1141 gcaacctctt cttcggcggc tgcgccgacg tggtcgtcaa atggacgctc gagcaccgct
     1201 cgatgaacgt cgggatcaac gagggcgaca tcttcctcca ggacgacccc tggatcggca
     1261 ccaaccacca gatggacgcc gccgtctacg cgccggtctt ctgggaaggc aagctgttcg
     1321 cctgggtcta caacgtgatc caccagcgcg agctgggcgg ggtcgagccc ggcggcttca
     1381 cccagcaggc ccacgacgtc tacgccgagc cgaccttctt cccgccgatg aagctggtcg
     1441 acgccggcaa ggtgcgcgag gacgtggtcg acgcctgggt gcggcgctcg cgccttgccg
     1501 ggctgatgac gctggagctg cgctcgcaga tcgccggggt cgaggtggca cgagcgcgcg
     1561 tactggacct ggtcgagcgc tacggcgcgg cgcgggtcaa gggcacgatg cggaagatga
     1621 tcgacacgac cgcgagcgtg gtcggcgatc ggctggccag cctgcccgac ggcgagtgga
     1681 ccgacacccg ctacgtctcc ggcgccgtcg tcggcgacct cactccttac aagtacagcc
     1741 tcacggtgcg caaggagggc gaccggctca cctactcgaa caagggcacc gatcccagcg
     1801 tcggctcctt caacatcacc gccggggtgc tgcgcgcctg catcaccaac tcgctgctca
     1861 ccttcctctg ttacgaccag tacctctgcg ccgccgggct gctggagcag gtcgacttcg
     1921 agttcgagcg cggcacgatc accgccgcgc accacccggc cgcggtcagc acctcgctgg
     1981 ggctggtcgt cgcattggtc caggcccagt acctgaacgc gaagatgctc tcgaccaacg
     2041 aggagaccgc ccaccacacc ttcggcgtct ccggctgcgc gacgctgatc tacaaccaca
     2101 ccttcggggt cgaccagtac ggcaacccga ccgcgaactt cccgctcgac gggatcgtcg
     2161 gcggcctcgg cgccttcccc tggcgcgacg ggctcgacca cggcggcacg atgagctcga
     2221 ccatgaaccc ggtcggcagc gtcgagtcga tggaacgcga ggtgccgatg ctctacctct
     2281 accggcgcga ggcaaccgac tccggcggcc acggccgctg gcgcggcggc gcctgcatcg
     2341 tctcggcgat ggtcggccac cgctcgcccg agcactacat ctcctccggc ggcctcgccc
     2401 agagcgtcac ccagggcatg ggcctgctcg gcggctggcc ggcgaccggg gggacgatgt
     2461 ggcgggccga gaacaccgag atccgcaagt ggctggacgg cggccgcgtc ccgggcacgc
     2521 ccgacgagct gcgcgagatg gcccccgacg gcgacctcgc ggtgcccaag gtcttcaaca
     2581 acaagctgtt cgaagacgac gtcttcgagg tgatcccgaa ccccggcgcc ggctacggcg
     2641 acccgatgct gcgcgaggcc gagacggtcg ccgccgaagt cgtcgagggc cggctcacgg
     2701 ccgcggaagc gcgggcgatc tacggcgtcg acgtcaacga ggccggcgag ctggccgcag
     2761 gtcacgagga gttccgccgc gaacgccgcc aggcgcggct cgccgaggcg aagccgccgc
     2821 tcgaggcgac cgagggcaag atcgccgagg cggagggtct ggccctggcc agcgtcgcct
     2881 tcggcaaggg cgatggggga gagcagctgc tcggctgccg cgagtgcggc cacgctctgg
     2941 ccgagcgcca gggcaactac cgcctcggcg ctgcccgcct ggagctggcg atgaccgagc
     3001 tcggcgccac cttcatcgac ccgcaggagc aggtcggcca cgagctgatc ttccgcagct
     3061 acctctgccc gggttgcggc accgccctcg acggcgaggt ctgcaagccg gacgatcccc
     3121 cggtctggga cgtgcggttg gcgccgggcg gctgagcgga cgggcgtacc tcggaatcgc
     3181 gtgctcctca cgggccatcc cgggggttcc gtccggctca cgagtctgct ttagaccccg
     3241 catagcgggg tcagctgcag actcgcggaa gttcagggat cgcgtggttc cttttcggac
     3301 atagggaccg gaaaggaacc gcgcgacgga agccgggccg cacgcacacc tacctgccct
     3361 tggaggtgtg cgggtggccc ggcggcttgc ctaatcggcc ggtccgccgc cgtagccgag
     3421 ctcgttgccg tcggggtcgc ggaagatcgc cttgcggacg ccgttcgaat aggtctcgac
     3481 ctcggccggc tcgagcccgc gctcggcgat cgccgcgagc agcgcgtcga actcggcgac
     3541 gaacacggtc gggatcgcgg tcccggcccg cttctcgtcc tcgacgatga acaggtagcg
     3601 gtgctcggcc agctcccaga ccgcctcggt ctcgtgcggc atgaacgccg gctcgccacc
     3661 cagcagcgtt tcgtaccagg ccttcgccgt tgcgaagtcg ctgaccggca tccctgcgaa
     3721 cagatcgacg gacacgctcc aaggataggg aggggtggtc aggttcaccc ctgggggtcc
     3781 tggttggggc ggcgcttctc gccgagcatg gtcattgcaa cgaagcactc cgcttcgcaa
     3841 ccaacttcaa aggagaaacg ttatgacccg caagaccaag ttccgccgtt cgatcaccgc
     3901 cctcgccgtc accgccctgg ccctcgccgg actcggtggg agcgccgccg gcgccgccgc
     3961 tgcgaccccg accaaagtcg cgatcttcgc cgagtcgggt ggcttctacg gcttcgtcaa
     4021 gagcgccagc cccgaatgca aagttgaacg gaccgtcgcc gtctacaagc agctcggcgc
     4081 caagcagcgc ccggccaccg acgagcgcgt cgccatggac acgaccggcg aagacgggga
     4141 atggaacatc ggcaaccccg gcctgcgcag cggtcgctac tacgcccgcg ccacggtcaa
     4201 gcccggctgc aaagcggcca acagcatcac cctgcgggcc cagcagtagt ccccgcagca
     4261 acccacgctt cctcccaaac ccaccgcccg gcggctcccc ttggccgccg ggcggttccc
     4321 ccatttctag ggcaggaatc ttcgtccgaa cgtccaatta gcgtctatat cgaacggtgt
     4381 tcagtatgtt ccgcgcgctt tggcagcgga tcacgacggg cggaggaacc cttgaaagcg
     4441 aagtcttggc tggcagttct cgtattcacc tgtgtgctcg gggtagctcc cgcgagcgcc
     4501 ctggcggggt cgatcagcgg ctccctgacc gacgcggaga ccggcgcacc ggtgaccgac
     4561 tccctggtct gcgcctacgg cgtgaacggc ggtggctatt gcgtcggcgt gccggccaac
     4621 ggcgagtacg cgatcggcag cctccccgct gacgaatacc gggtcagctt ccgtgcctat
     4681 ccgggcaaca actacgcccc ccagtactgg caggaagccg acatatggga cgacgcgacg
     4741 ctgatcgccc tgggcgccga cgaggatcgg accgggatcg atgccgcgct cgaacccggc
     4801 gccatgctca gcggcacggt cgtcggcgaa ggcgagatcg gcccgctgga gggagttcag
     4861 gtctgcacga cggatctggt ccggcccggc gggctgatcg aatgcgtaga aaccgacgcc
     4921 gacggcgagt accagctcac cggcctgcgt tccggcagct accgggtgac cttttcgacg
     4981 ccctggtcgt cggtcgaata cgcgacccgc tactacgacg aaaaggcgtt cgaagacgaa
     5041 gccgacgagc tgcagctgac cgcgggcgag tcggccaccg ccgacgcgac gatgagcccg
     5101 gcgggcgcga tcgaaggcac ggtcaccggc gaaggcgaac cgctcgaatg ggcgtgggtc
     5161 tgcgcttaca ccctcgccgg ggacaagctc ggctgcgcgc cgaccgacga agacggccac
     5221 taccgctacg gcggcctgcc cgccggctcc tacgtgctcg aagtcgaagg ctatggcttc
     5281 atgcctgagt tcagcggtga cgcgagcacg ttcgaggagg cgacgccggt caccgtcctg
     5341 gccggggaga cgacgccgtt tgacgccgac ctctccgccc cgcccgggat cgaagggacc
     5401 gtgaccgccg ccgccagcgg cgaaccgctc gaaagcgtgc gggtctgcgc ggttcccgac
     5461 ttcgaaagca cgaccagctg cgtgtacacc tgggaagacg gcagctacaa gatcgaaaac
     5521 ctcgggaccg acagctacac ggtcagcttc gaggccgacg gctacgcgat ccagtactac
     5581 gacggcgtgc tcgccgagtc cgaagcgacc aaagtcccgg tcagcgatgg gatggtcagc
     5641 gggatcgacg ccgagctcgt tcccggcggc ttgatcaaag gccacgtgac cctcgcctcc
     5701 tccggcgcca acctcggcag cgtcaaagcc tgcgcgctgc gcgccgacgg cagcgaagcc
     5761 ggctgcgaca gcacggcgac cagcagcggg atcttcgaaa tcgaggacat cccggtcggc
     5821 gagtacaagg tgcggttcac gcgcagctcc tacgtcaccc agtacttcaa cggcaaggcg
     5881 accttcgaag ccgccgatcc ggtgaccgtt gccgccggcg agacgaccga aggcgtcgat
     5941 gccgcgatgg tgaaactcga caaaccgaaa aacaccgcgc tgccggcgct gaccggggtc
     6001 gggaaagtcg gcgaaacgct tgcctgctcg gacgggacct ggtcgggaaa ccccagcaaa
     6061 tacgaatatt attggtaccg caacgggttc gaaatccagg gcgcgttgct cagcacctac
     6121 aaactcgtca gcggcgacgc gggcaaaacg atcagctgtg cggtcgaaac ctggaacagc
     6181 gcgggctggt cggaccaggc tctcgcaccg acctcgatcc aggtcgcggc gatccgccaa
     6241 ctcaccgtct ccaaggctgg caccggctcg ggttcggtga ccagcagccc gtccgggatc
     6301 gattgcggtt cgacctgcag cacgagcggc aaccagggcg agacgatcac cctgaccgcg
     6361 gctccggcgc agcactccca gctcagcggc tgggcgggtt gcacgacttc cagcgggacc
     6421 gaatgcacgg tcacgctcgg cggcgccaac gccagcgtca ccgcgacctt cgccccgatc
     6481 acccacaccg tctcggtcgc ggtcaccggc ggcggctcgg tcagtgcggc cagcggcgcg
     6541 atcaccggct gcaccccgag cggcggcgcc tgctcgggca gctacgacga gacctccgaa
     6601 gtcgtgctca ccgcgacccc gaatgcgcac cagtccttca ccggctggac gggttgcacg
     6661 accgcagccg gcaccgaatg caaggtcacg gttgacgggg ccgagaacgt gaccgccggc
     6721 ttcgcgccga tcctgcacga gctcgaggtc gcgaagaccg gcggcggctc gggcagcgtg
     6781 accagcaacc cggccgggat cgactgcggc tcgacctgcg cggacgagtt cgaagagggc
     6841 agcacgatca cgctgtccgc gaccccggcg cagcactccg agttcaccgg ctggtcgggc
     6901 ggcggctgca gtggcaccgc gacctgcgcg gtcacgttgg gagccgacat cgaggtcagc
     6961 gccggcttcg ccccgatcac ccatggcgtc tcggtcgcgg tgacgggcgg ggggtcagtc
     7021 agcgccgcca gcggcgcgat tgccggctgc accgccagcg ctggcacctg caacggcagc
     7081 tatgacgagg gcgccgaagt cgtgttgacc gcgacgcccg ccgcccacca gtcgttcagc
     7141 ggctggtccg ggtgcaccac cgcgaccggg gccgaatgca aggtgaccat cgacggcgcc
     7201 gagagcgtca ccgcgacgtt cgcgcagatc acccatgagc tgagcgtctc gaaggccggc
     7261 accgggtcgg gcagcgtgac cagcaccccg gtggggatcg actgcggttc gctctgcgcg
     7321 accagcttcg acgagggcga ggaggtcggg ctgaccgcta cgcccgccgc cgactccgaa
     7381 ttcaccggct ggtccggcgg cggctgcacc ggcacgggca cctgcacggt gagcgtggaa
     7441 gccgacaccg cggtgaccgc gaccttcgcc aagaaggcga ccgaaggcgg cacggtcatc
     7501 cccccgccga cgccgcccac cccgggcccg gccccgaagc ccaagccgaa gccgctgaag
     7561 tgcaagaagg gcttcaagaa gaagaaggtg aagggcaagc agcgctgcgt gaaggtgaag
     7621 aagaaacaga gcggcaagcg cgcctagcgc ggcccggggg gccggggaac cggcctccct
     7681 agagttgccg gggtggatta cggacacgag ctcgagttcg gcgtcttcat caccccggca
     7741 aacgagcggc ccgagggggt cgtgcggctg gccgaactgg ccgaggagct ggggctcgac
     7801 ctggtcagct tccaggacca cccctaccag tcgcgcttcc tcgacacctg gacgctgctc
     7861 tcctacctgg cggcgcgcac caaccgggtg cggctggcgc cgaacgtgct caacctgccg
     7921 ctgcgccagc cggcggtgat cgcgcgggcg gcggccagcc tcgacctgct cagcgacggc
     7981 cgggtcgagc tggggctcgg cgccggggct ttctgggacg cgatcgaggc gatgggcggg
     8041 cggcggctct cgggcggcga agcggtcgac gcgctggagg aggcgatcgc ggtgatccgc
     8101 ggcatctggg acaccggcga gcgcggcggt gtgcgcgtcg agggcgagca ctacaaagtt
     8161 cgcggcgccg cccgcgggcc ggccccggct cacgagatcg gcctctggat cggcgcctac
     8221 aaaccgcgga tgctgcgcct gaccgggcgg ctcggcgacg gctggctgcc gagcgtccct
     8281 tatatggagc ccgccgacgt cgcggccggc aacgcggcga tcgacgccgc ggccgagcag
     8341 gccgggcgcg acccgcgcgc ggtgcgccgc gtgctcaacg tctccggcgc ctactccgac
     8401 ggcactcccg ggccgctctc cggctccgcc gagcagcagg ccgaggagct ggcgcggctg
     8461 gccctggagg agggggtctc ggtcttcgtc gccaccggcg acgacccggc cgaggtcgac
     8521 cggttcgcaa ccgaggtggc gccggcgacc cgcgagctgg tcgcggccga gcgcgagcgg
     8581 cgtgtggagg aaggcccgag cgcgacggtg acgctggccg gttccggcgc cgatgccccc
     8641 agcgccaacg gcgcctcggc gaccggcgcg ccaccgcaga gcgacgaagc cggcgccccg
     8701 accagcgagt acgagcggct cggcgtcacc ccgacccccg acgacggcaa ccggctggcc
     8761 gcgccgcagt gggacgagtc gacccggccc caccgcaccc cctccggccc cgaggtcagc
     8821 tacaccgatg ccggccgcca cgccggccag cacctgatcg acgtgcacga ccacctgcgc
     8881 aacgagctca cccagctgcg cgaagtcgtc gcccaggtcc gcgacggcgc gctcggcgcc
     8941 ggcgaggcgc gcgacgccct gcacgcgatg gcgctgcgcc agaacgactg gacgctcggc
     9001 gccttctgca gccgctactg ccgcgtcgtc acccagcacc acacgatcga ggaccagtcg
     9061 gtcttccccc acctgcgcgg ctccgactcc gagctggagc cggtgatcga ccgcctgacc
     9121 gaggagcacc tgacgatcca cgacgcgatc gacgcggtcg accgcgccct cgtccaccac
     9181 ctcgagcacc ccgacgactt cgaccgcctg cagaccgcga tcgacgtgct caccgacacc
     9241 ctgctctcgc acctctccta cgaggagcac gagctggtcg agccgctcgc ccggctcggc
     9301 ttccagccga gccggctcta gggtcgcctg ttggcgcacg agcttgaatg gggggcccgg
     9361 gcgctacccg agcccccctg gagaccgccc ctcggctcag ggggccccga ctttgagaga
     9421 cggccgaacc tgagggcacg gtagcaaatt ttccggactc atgaccccgt ttctctagac
     9481 tctcaaaaac tcgcactttt gcttgagcag cctaatgagg caaagcacct tgtaagaagg
     9541 gcaaaagaac tctatattta ggtcatgagc tttccggaga ctccaagcca tgtctatgtt
     9601 cctgttcccg tagatcgggt cgacgacgtt tttcgccttc tgctcggcct caagcagcgc
     9661 gggggcgagg atgtccccga gcatctgctg caacgcgcct actcggaaag cgacacccag
     9721 tttcaggcgt tgcttcgcta cctcgccgag cgccctgacg aggcgatcgg cagcgtccag
     9781 attgccaacg agctgggctt ggcgaacggg accgcttcgc tggccggcat gctcggcgcc
     9841 tttgctcgcc gtgcgaaaaa ccgctacgac ggcttctggc cattcgagcg gatctacagc
     9901 cccggcgacg aagggtcgcg gctgattatg accgcggaga tcgcgacggc gatcaagcgg
     9961 ctcgccgcgt agcgcgatcg gcgtcggtcg cggcgagcat cgcagcgatc tcccgcgccc
    10021 gggccgccgg gtcctcgtgc tcccagaccc ggatcacggt ccagcccgcg gcgttcagct
    10081 ccgcgtcgtt gcgacggtcc cgctcgacgt tgcgggcgat cttcgcgctc cagtaggagg
    10141 agttcgtctt cgggctcgtg ccgtgctcgg ggcagctgtg ccagaagcag ccgtcgacga
    10201 agaccgcgac tcgctgccgg cggaagacga cgtcggcttt gcagcgcagc tcccgcagtg
    10261 gccgggtgtc gacgcggtaa cgcaggccga gccggtggag agccgagcgc agcgccagct
    10321 ctggccgggt gtcgcgcttg cggttccctt tcatcgcggc ttcgaccgcg ggcgacgaag
    10381 ccgggggtgg cgccgggacg gtcgttaggt tggcggggtg gatgcgatct ctcttttctc
    10441 gggggccgga gggcttgatc tcggctgcga ggcggctgga ttcgagactg tcggagtcgt
    10501 cgaggtggag gaggtagcgc gccagacgat gcttgcaaat cgcgaggatt tcttcccgcg
    10561 tctgcgcgag gaagcggtgc tcaccgacat cctcgaggcg aaccccggcg atctgctcga
    10621 tcgagccggg ttgcagccag gcgaagtgtc gctgctgcat ggcggaccgc cctgcacgcc
    10681 cttctcgaag tcgggctact ggctcgccta caagcgcgct ggcgaagacc cgcgggcgag
    10741 cttgctcgac gagtacgtac ggatgctgcg tggcatcaag ccgaaggcct tcctgatgga
    10801 gaacgtctac ggcctcgcct atcgaaacca gaaccgggcg gtcttcgagc gcttcctgag
    10861 cgcggcgcgt cgtgccggct acgcggtcga tcaccggatc ttgctggcgg ccgacctcgg
    10921 cgtcccgcag cttcgtcagc gcctcttctg tgtcgggatc cgccgcgacc tgctctcggg
    10981 ttcttcgcga ggctgggaga tggagtggcc ggcgagcacc cactctggcc cgcacgagac
    11041 tcgaactggc tgggatgagg ggttgcttcc ccacaagacg actggagaag ccctcgaatc
    11101 gcttcccgag aggcttaacc cagaagagcc cgaggagatc gtcgagggaa ccttcgccga
    11161 ttccctgcga gccgtgccac ccggtgacaa ctatctcttc tggaccgccg agcggggcca
    11221 taaggagcct cgattcaaat ggcgctcgcg ctactggagc ttcctgctga agctccaccc
    11281 ggacaagccg tcgccgacga tccaaggaca gccaggtccg tgggttgggc cgttccactg
    11341 ggagggccgc cgcctccgag ttgcggaggt gaagcggctg atgaccttcc ccgacgagta
    11401 cacggttctg ggcgcccgca gagagcagca gctccagctc ggcaacgcgg tgccgccgga
    11461 cctgggccgc gccgtcgctc gcgcgattgc cgaggaattg tctcggctgg actccgcctc
    11521 cgctccaggc cttgcgttgg cggcataggc gatccgcgat cccggttagc tttcgggaat
    11581 ggggatacgg cttgaagacg aagcgctgca gctcaccctt gagcaagcgc tgagcctggc
    11641 cagatcaggc aagccgcttc ccacgatctg gctggaccgg gttgagcggc tcggtgagct
    11701 gggggtcaag acctatatcg ccgccctggg aggcgcactt ctggcgaagg cgactaaccc
    11761 tgaagtcgac agcctgctcc aggacgagaa tgcggggaat ggcggttaca gcctacgtag
    11821 ggccgcggaa ttcctcgcgc ttcacaacga aggtcgctac cacctaggcg cgcaaggacg
    11881 gtggcctctt aacaaccgcc cgtttctcgg tggcccctcc cggatcgatg agttcaaaaa
    11941 gatctcgggg cgcgctcgcc catcctatga gttgtttctg gacacgcttc gcgatctgcg
    12001 aagacgtgac gagcctgaag ccctcgaagc gctagctgca ttcttgcggg tgcggatgca
    12061 ggtgcaggag gaagcccgaa ccgccgcgcg tgaagcgcgc ggtgtcgaaa gcgatcttgc
    12121 gatcgccgat ttggtcaggg tcatcgacct gtttgtccat cgggactctg aaggtggaaa
    12181 acgagcccag gctctaaccg cggcggtcct ggattgtgcg ttcgatgagg tcgacatggg
    12241 gccaatcaac ggcccccgcg cgggtgacgt gcgcgttcta tccaacgatc gggtgatcgt
    12301 ggccgcggag gccaaacagc tggaagcaga ggatgccgac gcgaccaagc tgtcgagcga
    12361 cgcggccgag ctcggggccg acctggcatt gctcgtagtg ctcttcaagc gtcaccggtc
    12421 cctcgatcgc gaggcccttc gacaggcggc gttgcgcgag gacggcactc tgttggtcat
    12481 ctgcgagagc gtgattgagc tggtcacgtc ggtcgccgtc tactcggcga ctccggctgc
    12541 tcacatcgag acggagttgc ctggcgccta tgcgctgcga atgcgcgaga tcgaggtctc
    12601 cccagcggcg ctggaggagt gggagcacca gatcaaagcg cgtaccggtc gctagctttt
    12661 cggcagcgcg aactcgaccc agagcgggta gtggtcggag atccggtggg acttgtcggt
    12721 tttcgacatc cccgatttgg cgagtacggt cgtgctccag tcgaacacgt tcgctttccc
    12781 cgtgtagtcg agggagatca gcgatttcct cccgtcgttt tcgtcgacga accaggagat
    12841 ctggtcgtag aagcccttca gcggtttgtc ttcgacgatc cgcggctggt cgatcggcga
    12901 cggggtggtc agcccggtcg aggtgaacgc cttccacagc ttcccgtctt ttttgtcgat
    12961 gttgaagtcg ccgaggcaga tcaggttctg gtcccagctg ttggtgtcct cggcccagga
    13021 gcgcagccag gtcgcgatcg cggtcagctc cgcggttcgc gcccggatcc cctgtttccc
    13081 ggtgccccag aacacgtgca gagtgaccag gatgaaggtg gagttgccgc gccggaacgc
    13141 gaccgcgtag ggggtgcggg cgaactggcg tttcagcgcc acccggtcga tcccggcttt
    13201 cggtttccgt tttttcgtcc agttgagcgg gacgaccagc tcgcaggcga ggccggagac
    13261 ctcgagccgc ttcttgttga agacgaaggc gagccgctcg ttgttgcctt tgtggtcttc
    13321 gaccgcgtcc gtgaggatca cggtgaaatc gctgccgagc agcttcagca tgtggcgcag
    13381 ggcccggatc tccgtctgca cctcctggat cgcgaccacg tcgaagcggg agacgatctc
    13441 ggtgatgcag gcgagggcgt gcaggtcgcg cttcggcctg tcggctttca ccgagtgcca
    13501 tttgtcggtc aggccgccaa acttgcggat gttccaggtc gcgaccagga ggttgttccg
    13561 cgtgcgccgc ggcactttct cgtcgagccg ggcggacagc gcggcgagct ctttgacggt
    13621 gtcggccggc ggtttgtcgg tgatcgcggc ctgcgccatc gggcgaccgt atagaactct
    13681 cccgtactta ggcagaactt ttggagtact taactgtcag cgagcgttcg ctcagccggc
    13741 gagcacgtcg taggcgaacc gcaccgtgag ggcgccgtcg ccgaccgcgc cggcgacccg
    13801 cttggcggcc ccggcacgca cgtcgccagc ggcgaagacg ccggggcgga cggtctcgag
    13861 caggtgcggc tcgcggtcgg agagcggcca gcagaggtgc tgggggcagg cctctgcgcc
    13921 ctcgccgcag agcaggtagc cggcctcgtc gaccgcgagg atgccgtcgc aggcttcggt
    13981 gcaggggtcg gcgccgatca tcacgtagac ggcggtggtg tcgacctcgc tggtcgtgcc
    14041 gtcgcggttg tcgcgcaggg tcaccgactc gaccgtggat ttgccctgca gggcggtgat
    14101 ctcggtctcg gtcatcacct cgatccgcgg gtgggtcttg atccggtcga tcaggtagtg
    14161 ggacatcgtc gacttcagcg ccttgccgcg gacgacgacg cgcacgctgc gcgctcggtt
    14221 ggcgaggtgg acggcggcct ggccggcgga gttgccgccg ccgaccacga tcacgtcttt
    14281 gtcgcgcgag cgctcggcgt cggtcgacat cgccgcgtgg tagacgccgg cgccgcggaa
    14341 gcggctcatg ccctcggcct cgagctcgcg ccagcgcgcc ccggtggcca tcaccagcgc
    14401 ccgcgcgagc acgtgctggc cgtcgtcgag gtcgacccgg atcaggccct cgggcccgtc
    14461 ggcgagctcg accgcgcggt ggaagctgga gatcacggcg tcgaagcggc gcgcctgcag
    14521 ggtcgccttg gtggtcagct cgcggccgcc gatcccgctc gggaagccga ggtagttctc
    14581 gatccgggtg ctcgtcccgg cctggccgcc gggcgcccag gactcggcga cgaaggtgcg
    14641 cagcccctcc gagccgccgt agacggaggc cgccagcccg gccggcccgg cgccgagcac
    14701 gaccaggtcg aagcgctcgc cgtcgacctc ggcgcgcagc ccgagctgcc gcgccgcctg
    14761 cgcgggggag gggttcctga gcacggtccg cgcgtgcacg agcaccggcg tttcctcggc
    14821 tgggatcccg agccagtcga gcatctgggc gctctcgggg tcgttgtcga cgtcgtacca
    14881 gcggaccggc aggaggttgc gctcgagcag gtcgcggact tcgaaggcgc gccgcgagcc
    14941 ggccggcgcg atcagccgca tcaccccgtg cccgccggct tcgtgccact cgcgccgcgc
    15001 cagcagggtg ccgaagatgt gctcgccgaa ctccggccag cgggcgacca ggtcgcgcag
    15061 gccggggcgg tcgaaggcga gggtcacggt cggctcgacc gcgacgcagg cgctgatcgt
    15121 cggctcgccg gtgaagatcg cgatgtcgcc gataaaggtg ccggcgtcgg cctgggcgat
    15181 gtggacgttc ttgcccggct tgcgatcgac gaactcgacc aggccgctct cgatcacgaa
    15241 gaaaggcgcg tcgcggaccg cgtgctcgta gagcacctcg ccgggctcga agcgcttctc
    15301 gaccgcgccg cgctcgcgca accaggcgaa cttcgcctcc gagaggcgcg ggtagatctg
    15361 ggtatcggag aggtccggga agttctcgct gcggactact ttgatctcgg cttgggcctc
    15421 aggcacagag cgaggctacc gggccgcgcg cctcgctcaa agcagtggct cgcgatcggc
    15481 ctcgaccagc gccagccagc gctcgccggc gccgtgctcg cggtagatcg ccgcggcccg
    15541 gtcgagcttc tcggtcgccg cgccgcgctc gcccgcaccg gccagcgctc gcccccactg
    15601 gtgcagggcg tcggcctgct cggcgggcag tcggtagcgg ccgaaggtct ccagggcgcg
    15661 agcgaaggcg gcctcggcct gatcgggccg gtcctcgccg gcgagcacca ccgcctcggc
    15721 cagcgccacc tgcccgcccc ggccgcgcca gtcctcgccg ttgccgacga tctccaggca
    15781 gcggtcgagg tgggtgcgcg cgaccgcgag gtcgccggcg tcggcgctga cccgggcctg
    15841 ctcgatccgc acccagagct cgaagtagcg ggcgccgccg gggccgacga tcgccgccgc
    15901 gcgctccagg tactcggccg cgggcgccag ctccccacgc aggtggtgga cgtgggcggc
    15961 gaggtgctgg gaggaccact cgtcccagcg gttgccgttg cggcgactgg tctcgagcgt
    16021 gcgggtggcc agcgccgcga cctcgtccca gcgcccgtcc cagaggtcga gtaagggttt
    16081 caatgcgtgc gtcatccagg ccgggcgggc gtcggagagc agggcgcgcg ccgggccgag
    16141 ctcgccgcgc gagaaatggc agcgaccgac cccgtcggcg gtctcctggc ggaacgaggt
    16201 ctcgcccacg taggggaggg cgatgtggcg ctcgaagaag ccctgcgcct cgtcggggga
    16261 gcagaggccc cagctcatct ggccgcggat gttgagcgcc atccaggcga gcagggcgcg
    16321 gcgctcgcgg tcgacgacct ccagggcccg ctccatctgc gcgaagccct cggcgagctg
    16381 accggcggca atcaggtgcc agccgcccga ctcggcgcct ttcgcccaga gcacctcgtc
    16441 gccggtccgt tcgccgatct ccatcgagcg ggcgcccgcc tcgatcccgc gctcggtctg
    16501 gaccccgtag gtgagcgcgg ccgcgacgcc gccttcgagg tggccgagcg cccggcgcgg
    16561 cgggccctgc tcgaacacgg cgcgggccgc gtcgaagtgg cggaaggcga cgttgaggtc
    16621 catgtggtcg gcgtagatcg agtcaatcag cgagtaggcg ctgcccagcc gcgagtgcgc
    16681 ctgcgcggtg cgctcgacgt cgccgagccc ttcgaacagc gccagggcgt cctcgaggcg
    16741 agcgatctgg ccgccgacgt cgccggccac cccgagcagg tcgccgagca gcaggtcgag
    16801 ctcggcccgc tcgcgctcgc gccccgcgac cgcggccatc accgccagcg cgccttccca
    16861 gtgcgccgcc gcctcctccc aggcggagac ctcggccgct tcgccgccgg cgcggaccga
    16921 gtaggcgacc gctttctcgg gatctcccgc cgccccggcg agccggtact ggacggcgag
    16981 cgcggcgatc ggcggctcgc ccgcggtcgc ctccagcgcc gccgcggcgc gcgcgtgcat
    17041 gcgctggcgc cgcgggccgc tgagttcgct gtagagggcc tcgcggacga gggcgtgggt
    17101 gaacccgtgg gtggcggcgg cgccttcggc ctcgaccacc agctgcgcct cgaccgcctc
    17161 ctccagcgcc gcgatcagcg cctcctcgtc ggcgacctcc atcgccagca gggcctcgaa
    17221 gcggaactcg cggccgagga ccgcggccgc ggcgagcgcc gcccggcagt tctccgagag
    17281 ccgcccgagc cggccgccga gcacgtcgcg gacgccctcc ggcacgccga tctcgtccgc
    17341 ggtcagggcc gaggtccagc ggccgccgcg ctcgaagagc acgccggtct cgatcaggtg
    17401 gcgcatcacc tcctcgacga agaacgggtt gccctcggtg tgctcgtgca ccgtcccgac
    17461 cagcgccgcc ggcgccgcgt gcccggcgtg cagggcgatc agctcgttga ccccgccctc
    17521 gtcgagcccg tcgagcgaga cccggtcgaa gaggcggtcg cggcgcaggt ccgcgagcag
    17581 gtccgcgagc gggtgctcgg gcccgatctc ggcgtcgcgg taggcgccga cgatcagcag
    17641 cgagccctcc tgcgaggagc ggatcgtgtg ccgcagcagg tgcagggtgg cgcggtcggc
    17701 ccagtgcagg tcgtcgagca cgagcagcag cg
//
LOCUS       DASRIG010000274         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_662363, whole genome shotgun sequence.
ACCESSION   DASRIG010000274 DASRIG010000000
VERSION     DASRIG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662363"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="gnd"
                     /locus_tag="VFB52_07530"
     CDS             <1..972
                     /gene="gnd"
                     /locus_tag="VFB52_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZO06223.1"
                     /translation="ARRLMKDGHEIVAFDVNPDAVAELAGEGATGAESLADLAAKLSA
                     PRSVWVMVPAGGITEQTIKDVAAVLEPGDAIIDGGNSYYRDDIRHAQMLEQDGIDFID
                     CGTSGGVFGLERGYCLMIGGPDGAVERLDSIFASLAPGVESAERTPGRSGEVTPAENG
                     YLHCGPNGAGHFVKMVHNGIEYGIMAAYAEGLNVLKNANAGKVQREADAETAPLDHPE
                     YYQYDLDLPEVAEVWRRGSVVGSWLLDLTAAALAESPELSEFSGRVSDSGEGRWTAIA
                     AIDEGVPTPVLTTALQERFFSRELGDFGDKVQSAMRKQFGGHDEKPS"
     gene            983..1663
                     /gene="pgl"
                     /locus_tag="VFB52_07535"
     CDS             983..1663
                     /gene="pgl"
                     /locus_tag="VFB52_07535"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZO06224.1"
                     /translation="MAPKLEVAGDERAAARRAAELIAEAGAAAVADRGFFGFAMSGGR
                     SPWAMLAILGELEQMPWAETELFQVDERVASPGSEDRNLTHMVLGLSMDHQASLRPMP
                     VTQRDLDAAAREYESSLPEPFDLVHLGLGPDGHTASLVPGDPVLEVSDRRVALTGTEY
                     QGHRRMTLTYPALDAARRILFLITGAEKREPLQQLLAGDHSIPAGRVSNDEILVVADE
                     AAAEGLSS"
     gene            complement(1680..2153)
                     /locus_tag="VFB52_07540"
     CDS             complement(1680..2153)
                     /locus_tag="VFB52_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06225.1"
                     /translation="MDRLVELHRALGRAGIGHAFGGALALGHYATPRATADVDLNVFL
                     APSEWPRVREALAALGADVEAARFDGSDQVRVPWDGAELHLFFSVDELHEAMAGATRT
                     VALDGAAIPLVAPEHLVVRKLLLDREKDHADVAAIRAATPLDEAEIERWLEHLST"
     gene            2229..3350
                     /locus_tag="VFB52_07545"
     CDS             2229..3350
                     /locus_tag="VFB52_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO06226.1"
                     /translation="MAKTATVATVPSPRAGTVEVLVGLLAELDEATESGVFYDRVCRA
                     VCTLTSMKRAALLLNDETYRAVIPMGSYGVDPELLKRIEGTLDETPIARLALEEDRVV
                     EVTGSLHGHVPARYAELADTPTITCTPISAARRWLGVIFADRGGEDFTLDGGERETML
                     TLGRLAALTASVEHSTEQRESERRLAERVELTRELHENAIQRLFGVALALGSERGLSA
                     EERRRCQEELRSVVADLRHALTRPVAGPPRGLQMSLRQLLERLLERRPELHLEWEPGV
                     EVPPDLEPLAQSVLNEALRNADRHAEPSQIEVRVASDGETFSLAVENDGSLDDRATKG
                     GGLGLRLAALEALRYEGLVEFGSPSPGRWRVRLVVPVVS"
     gene            3350..3955
                     /locus_tag="VFB52_07550"
     CDS             3350..3955
                     /locus_tag="VFB52_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06227.1"
                     /translation="MASEEGKLRVLVVDDHDVVQWGFRLLLERQSWVERCLAASSGEE
                     AVEVCRKLRPEVALVDMLLGSESGAEVCEEIRSVSPNTRVLLISGAGVISPNVARSAG
                     ASGFISKDWSAVDVVKAVRMVSLGTEVFADQAPLDSPLSEREQEVLSLIATGSTNKEI
                     ASHLHLSPHTVKEHTSAIYRKLGVRNRAEATRQAQRLKILV"
     gene            4231..4635
                     /locus_tag="VFB52_07555"
     CDS             4231..4635
                     /locus_tag="VFB52_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZO06228.1"
                     /translation="MILADDLRAELEAYAGQSGVKVLRRLLDRDTFVLSDEELERLFL
                     PLAREVGLSLPETKIWLNGFEVDFFWPELRLVVETDGLRYHRTPSAQARDLKRDQAHA
                     AAGYTRLRFSHWQVKYKPRHVKAILRATAARL"
     gene            4775..>5391
                     /locus_tag="VFB52_07560"
     CDS             4775..>5391
                     /locus_tag="VFB52_07560"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZO06229.1"
                     /translation="MASDTSQIAILFPGQGSQTSGMAAVTAAQRPDLLEQAREELGAD
                     PFEQIGEGTHFAQPALYCASLAHWKQAGSPTGAMLAGHSLGELAALVAGGALGAEQGL
                     RLAVVRGRLMEEAAQEAPGGMLASLGGEEPTVREIAGRFGLTVANENAPGQLVLSGPS
                     EGLGEARKELRASGAKAIRLPVAGAFHSPLMSGAAANFRAALDQV"
BASE COUNT          808 a         1980 c         1868 g          735 t
ORIGIN      
        1 gcccgccgcc tgatgaaaga cggtcacgag atcgtcgcct tcgacgtcaa ccccgacgcg
       61 gtcgccgagc tcgcggggga gggggcgacc ggagctgagt cgctggccga cctcgccgcg
      121 aagctgagcg cgccgcgctc ggtctgggtg atggtcccgg ccggcgggat caccgagcag
      181 acgatcaaag acgtggccgc ggtgctggag ccgggcgacg cgatcatcga cggcggcaac
      241 tcctactacc gcgacgacat ccgccacgcg cagatgctcg aacaggacgg gatcgacttc
      301 atcgactgcg ggaccagcgg cggcgtgttc gggctggagc gcggctactg cctgatgatc
      361 ggcgggcctg acggggcggt cgagcggctc gactcgatct tcgcctcgct ggcgccgggg
      421 gtcgagtcgg cggagcgcac gccgggccgc agcggcgagg tgacgcccgc ggagaacggc
      481 tacctgcact gcgggccgaa cggcgcgggc cacttcgtga agatggtcca caacgggatc
      541 gagtacggga tcatggccgc ctacgccgag ggcctcaacg tcctcaagaa cgcgaacgcc
      601 ggcaaggtcc agcgcgaggc ggacgcggag acggcgccgc tcgaccaccc cgagtactac
      661 cagtacgacc tcgacctgcc ggaggtcgcg gaggtctggc ggcgcggcag cgtggtcggc
      721 tcctggctgc tcgacctgac cgcggcggcc ctggcggagt cgcccgaact gagcgagttc
      781 tccggtcgcg tctccgactc cggtgagggt cgctggaccg cgatcgcggc gatcgacgag
      841 ggcgtgccga ccccggtcct gaccaccgcc ctgcaggagc gcttcttctc gcgcgaactc
      901 ggcgacttcg gcgacaaagt gcagtcggcg atgcgcaaac agttcggcgg acacgacgag
      961 aagccgagct gaggccgcgg gcatggcgcc gaagctggag gtcgccggcg acgaacgcgc
     1021 cgccgcgcgc cgcgccgccg agctgatcgc cgaggccggg gcggcggcgg tcgccgaccg
     1081 cggcttcttc ggcttcgcga tgagcggcgg ccgctcgccc tgggcgatgc tggcgatcct
     1141 cggcgaactc gagcagatgc cctgggcgga gaccgagctg ttccaggtcg acgagcgggt
     1201 cgcctcgccc ggcagcgagg accgcaacct cacccacatg gtgctcggcc tctcgatgga
     1261 ccaccaggcc tcgctgcgcc cgatgccggt cacccagcgc gacctcgacg ccgccgcgcg
     1321 cgaatacgaa agctcgctgc ccgagccctt cgacctcgtc catctcggcc tcggccccga
     1381 cggccacacc gcctcgctcg tccccggcga cccggtcctc gaggtcagcg accgccgcgt
     1441 cgccctcacc ggcaccgagt accagggaca ccgacggatg accctgacct acccggccct
     1501 cgacgccgcc cgccgcatcc tcttcctcat caccggcgcc gaaaagcgcg agcccctgca
     1561 gcagctcctc gccggcgacc actcgatccc cgccggccgc gtcagcaacg acgagattct
     1621 ggtcgtcgcc gacgaggccg ccgccgaggg cctgtcgagt tagtccccgt atagagcgac
     1681 taagtcgaca ggtgctccag ccagcgctcg atctcggctt cgtcgagtgg ggtcgcggcg
     1741 cggatcgcgg cgacgtcggc atggtccttc tcgcggtcga gcagcagctt gcgcacgacc
     1801 aggtgctcgg gcgcgaccag cgggatcgcc gcgccgtcga gcgcgaccgt tcgcgtcgcc
     1861 cccgccatcg cctcgtgcag ctcgtcgacg gagaagaaga ggtgcagctc ggcgccgtcc
     1921 cagggcacgc gaacctggtc tgagccgtcg aagcgggccg cctcgacatc ggcgccgaga
     1981 gccgccagcg cctcccgcac ccgcggccac tcgctcggtg ccaagaagac gttgaggtcg
     2041 acgtcggcgg tcgcccgtgg cgtcgcgtag tggccgagcg ccagcgcgcc accgaacgcg
     2101 tgcccgattc cggcccggcc cagcgcgcgg tgcagctcga cgagtctgtc catcacttag
     2161 aggtctatac gacacttggg cgtttctgac agctgtgtcg ggggcgcggg cgtagtctcc
     2221 tattggtcat ggcgaaaacc gccactgtcg ccaccgttcc ttccccccgt gccggcaccg
     2281 tcgaggtgct cgtcggcctg ctcgcagaac ttgacgaggc gaccgaatcg ggggtcttct
     2341 acgaccgcgt ctgccgggcg gtctgcacgc tcacctcgat gaagcgggcg gcgctgctgc
     2401 tcaacgacga gacctaccgg gcggtgatcc cgatgggctc ctacggggtc gacccggaac
     2461 tgctgaaacg gatcgagggg acgctggacg agacgccgat cgcgcggctg gcgctggaag
     2521 aggaccgggt ggtcgaggtg accggcagcc tgcacggaca cgtccccgcc cgctacgccg
     2581 agctcgccga cacgccgacg atcacctgca cgccgatctc cgccgcccgc cgctggctcg
     2641 gggtgatctt cgccgaccgc ggcggcgaag acttcacgct cgacggcggt gagcgcgaga
     2701 cgatgctgac cctgggccgg ctggcggcgc tgaccgccag cgtcgagcac tcgaccgagc
     2761 agcgcgaaag cgagcggcgc ctggccgaac gggtcgagct gacccgcgag ctgcacgaga
     2821 acgcgatcca gcggctgttc ggcgtagccc tcgcgctcgg ctccgagcgc ggcctctccg
     2881 cggaggagcg gcggcgctgc caggaggagc tgcgctcggt cgtcgccgac ctgcgccacg
     2941 cgctcacccg gccggtggcg ggaccgccgc gcggcctgca gatgagcctc cggcagctgc
     3001 tcgagcggct cctggagcgc cggccggagc tgcacctgga gtgggagccc ggcgtcgagg
     3061 tgccgcccga cctcgagccg ctcgcccagt cggtcctcaa cgaggcactg cgcaacgccg
     3121 accgccacgc cgagccgagc cagatcgagg tccgggtcgc ctccgacggc gagaccttca
     3181 gcctcgcggt cgaaaacgac ggctcgctcg acgaccgcgc gaccaaaggc ggcggcctcg
     3241 ggctgcggct ggcggcgctg gaggcgctcc gctacgaggg cctggtcgag ttcggatccc
     3301 cgagccccgg tcgctggcgc gtccgcctcg tcgtcccggt ggtcagctga tggcgagcga
     3361 ggagggcaag ctgcgcgtcc tcgtcgtcga cgaccacgac gtcgtccagt gggggttccg
     3421 gctgctgctc gagcgccaga gctgggtcga gcgctgcctc gccgcctcca gcggggagga
     3481 agcggtcgag gtctgccgca aactgcgccc cgaggtggcg ctggtcgaca tgctgctcgg
     3541 cagcgagtcc ggcgccgagg tctgcgagga gatccgctcg gtcagcccga acacgcgcgt
     3601 gctgctgatc tccggcgccg gcgtgatctc ccccaacgtc gcccgctcgg ccggcgcctc
     3661 cggcttcatc tccaaggact ggtccgcggt cgacgtggtc aaggcggtgc gcatggtctc
     3721 gctcggcacc gaggtcttcg ccgaccaggc cccgctcgac tcgccgctct ccgagcgcga
     3781 gcaggaggtg ctttcgctga tcgccaccgg ctcgaccaac aaagagatcg ccagccacct
     3841 gcacctctcc ccgcacacgg tcaaggagca caccagcgcg atctaccgca agctcggcgt
     3901 ccgcaaccgc gccgaggcca cccgccaagc ccaacgcctc aagatcctcg tctagcgggg
     3961 agacaccacc gcccctgtcc tcttatgcac gctatggcgt gcacgaaagg acaagcccgc
     4021 aaccggcaaa cctggcggct cgcgcgggcg cagcacggag tggtggcgcg gcggctgcgg
     4081 ccagggtgcc tgcctcagcc acagaagcgc cggagccctc ttcggcatct gcgaagagcg
     4141 caacggggtg gtcgaagtca gcgtgcgccg caccggcaac gtccaccgcg aaggcatctc
     4201 cgctcgatca acgaggccga caagcgcgag gtgatcctgg ccgacgatct gcgggctgag
     4261 ctcgaggcct acgccggcca atcgggcgtc aaggtccttc ggcgcctcct tgaccgggac
     4321 accttcgtgc tctcggacga ggagctggaa cggcttttcc tccctctggc gagggaggtc
     4381 ggactctccc tccctgaaac caagatctgg ttgaacggct tcgaggtgga cttcttctgg
     4441 cccgagcttc gcctggtcgt cgagaccgac gggctgcgct atcaccgcac cccttcagcc
     4501 caggcccgag acctgaagcg cgaccaagcc cacgccgcgg ccggctatac ccgcctgcgc
     4561 ttctcccact ggcaggtcaa gtacaagccc cggcacgtca aggcgatcct gcgtgcaacg
     4621 gcggctcgcc tctgacggcg tctgccgcat accgcccccg taccccgaac caggcacgca
     4681 ccgacccccc ggccgtcccc tctcatcccc ggattggggg ggaccgatgg ctcattaacg
     4741 tcaccttcgc cgacctccgg accactatgg ctaagtggcc tcggatacct cacaaattgc
     4801 gatcctcttc cccggccagg ggagccagac ctcgggcatg gccgcggtca ccgcggcgca
     4861 gcggccggat ttgttggagc aggcgcggga ggaacttggc gccgatccct tcgagcagat
     4921 cggcgagggc acgcacttcg cccagccggc gctctactgc gccagcctcg cccactggaa
     4981 gcaggccggc agcccgaccg gcgcgatgct tgccggccac tcgctcggtg agctggccgc
     5041 tctggtcgcc ggcggcgcgc tcggcgccga gcaggggctg cggctcgccg tcgtgcgcgg
     5101 ccgcctgatg gaggaagccg cgcaggaggc gccgggcggg atgctcgcct cgctcggcgg
     5161 cgaggagccg accgtgcgcg agatcgccgg ccgtttcggg ctcaccgtcg ccaacgaaaa
     5221 cgcgcccggc cagctcgtcc tctcggggcc cagcgagggg ctgggggagg ctcgtaaaga
     5281 gctgcgcgcg agcggcgcca aggcgatccg gctgccggtc gcgggcgcct tccactcccc
     5341 gctgatgagc ggcgccgctg ccaacttccg cgccgcgctc gaccaggtcg a
//
LOCUS       DASRIG010000275         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3801704, whole genome shotgun sequence.
ACCESSION   DASRIG010000275 DASRIG010000000
VERSION     DASRIG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801704"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFB52_07565"
     CDS             <1..457
                     /locus_tag="VFB52_07565"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZO06230.1"
                     /translation="VERLRNFFRENSVDESVAMGESGGLPPSQISGGSSGSPPDSRIE
                     AFYTALADDFNTPRAMAEVFELVSEANRGEVPGAAEAVYGMLEVLGLGNLAEDEDGAD
                     AEAQGLMLEREQARGEKDFARSDEIRDRLAALGWEVRDSAEGPRLVSRS"
     gene            458..1111
                     /gene="rlmB"
                     /locus_tag="VFB52_07570"
     CDS             458..1111
                     /gene="rlmB"
                     /locus_tag="VFB52_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZO06231.1"
                     /translation="MANQFIYGRQPVAEAERGRRRVHRVWRAPETSGVELERLCGSPD
                     HQGVVAEVDPYPYGDPVGLLRGENALLVALDEVQDPRNLGAVCRSAEVAGAAGVIVPE
                     RRAAAVTPVACKTSAGAVEHLQVAHVRNLADWLAQAKEAGFWIWGADADAESAPWEVD
                     LKGPTVLVLGGEGKGLRPRVAATCDGLIALPQRGQIDSLNVSAAATALLFEALRQRS"
     gene            1306..1971
                     /gene="sigH"
                     /locus_tag="VFB52_07575"
     CDS             1306..1971
                     /gene="sigH"
                     /locus_tag="VFB52_07575"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HZO06232.1"
                     /translation="MALSAPTQYQANAQAELDDHYLVALAKDGRPDAYDMIVKRYRGF
                     VRLKASSYFLLGGESDDLIQEGLLGLYKAIRDYRTDRESSFRNFAELCITRQIITAVK
                     TATRNKHTPLNQYVSFSQTPAAAGDSDTTLDEILPGPSVHDPVNQVIATEELESLVSC
                     LSGVSGVLSDLESRVLSLYLDGHSYEAIAERLECDTKTVDNALQRVKRKVGSHLASRE
                     VAL"
     gene            complement(1968..2951)
                     /locus_tag="VFB52_07580"
     CDS             complement(1968..2951)
                     /locus_tag="VFB52_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06233.1"
                     /translation="MSADEGHVLTGAIEAAYGRTYDVRWVYDPNEVAARLGDGRYVSC
                     VAETPDGELLCHEGMSLAAAGDAVAHSGQAVTMPLARGQHMFTRTKRHLMDWAQGEGL
                     AGMFSEATAAHPYSQKANVELGAHETGFLLGWIPATVANDAAAPAPRRRQSAALFYSK
                     LNDGHERQVYAPARHREIVGQTLALCELRGELADPPAGFDLPERTRFQVDVDDDHNLA
                     LITVTVPGADLAEVLRAERHHLFHARKLDAVYFDLPLETPATALVAEDLERLGISYAG
                     VFPNHRTDGDVLRMQSLHRVRVKVDDVAVASDHGRELLEYVLADLPAEKVA"
     gene            2993..3066
                     /locus_tag="VFB52_07585"
     tRNA            2993..3066
                     /locus_tag="VFB52_07585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3027..3029,aa:Thr,seq:tgt)
     gene            complement(3141..3770)
                     /locus_tag="VFB52_07590"
     CDS             complement(3141..3770)
                     /locus_tag="VFB52_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06234.1"
                     /translation="MKKLFKGRLTYANVVATLALCIAVGGASAFAATQLPKNSVTAKQ
                     IKNNAVTTAKLKKEAVTTAKIKNDAVNGAKVAAGSIDTTDLKAGTIPAPVNAYTKGES
                     DARYLHQATVTVVETIGTNIAPPGGFESGIVECPAGYQAIAGGVDNDGILNGKVSASA
                     PTIDGARTEAVADGEHGPPTGWYGAMTTQGGAANVTKAKIVVVCSRIGG"
     gene            complement(3855..>4214)
                     /locus_tag="VFB52_07595"
     CDS             complement(3855..>4214)
                     /locus_tag="VFB52_07595"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO06235.1"
                     /translation="AAWIHELRKQCIEEFPDAKFSDTFGGPRDRVVSAVKGANLGERA
                     AKLGESAKKVRLPEVPRSDGGETPTSEAPTTEAPTAVLGEDQRLARLERLGELREKGI
                     LTDEEFAAEKARVLGGE"
BASE COUNT          587 a         1383 c         1530 g          714 t
ORIGIN      
        1 ggtggagcgg ttgcggaact tcttccggga gaactccgtg gatgagagcg tcgcgatggg
       61 ggagtcaggg gggctccctc cgtcacaaat tagtggaggt tcctccggga gcccccctga
      121 ctcccgaatc gaggcgttct acacggcgct tgctgacgac ttcaatacgc ctagagctat
      181 ggcagaggtg tttgagctcg tgtccgaagc gaatcgcggg gaggtcccgg gggccgctga
      241 ggcggtctac gggatgctcg aggtgctcgg gctcgggaac ttggcggaag atgaagacgg
      301 ggcggatgcg gaggcgcagg ggctgatgct cgagcgggag caggcgcgcg gggagaagga
      361 ctttgctcgg tcggatgaga ttcgggatcg gttggctgcg ctcggctggg aagttcggga
      421 ttcggccgag ggtccgcgtt tggtctcgcg gtcttagatg gccaatcagt tcatctacgg
      481 gcggcagccg gtggcggagg ccgagcgggg gcgccggcgg gtgcaccggg tttggcgggc
      541 gccggagacg agtggggtgg agctggagcg gctctgtggg tcgccggatc atcagggcgt
      601 ggttgccgag gtggatccgt atccgtacgg cgatccggtg gggttgctgc gaggggagaa
      661 cgcgcttctg gtcgcgctgg acgaggtgca ggatccgcgc aacctggggg ctgtctgccg
      721 ctcggctgag gtcgccggcg ccgccggcgt aatcgtgccc gagcggcggg cggccgcggt
      781 cacgccggtt gcctgcaaga cctcggcggg cgcggtcgag catttgcagg tcgcccacgt
      841 gcgcaacctg gccgactggt tggcgcaggc gaaggaggcc ggcttctgga tttggggggc
      901 ggacgccgac gccgagagcg cgccctggga ggtggacctg aagggcccga ccgtgctcgt
      961 cctcggcggc gagggcaagg gcctgcgccc ccgggtggcc gccacgtgcg acgggctgat
     1021 cgcgctgccg cagcgcggcc agatcgactc gctgaacgtc tccgcggcgg ccaccgcgct
     1081 gctcttcgag gccctccgcc agcgctcctg agcctcctgc tcggcggtcg ccaggcgcgg
     1141 ggcaaatgca acggcgtttg catttctcgc aatttggggt tgacagaact ccgctgaacg
     1201 gtaaacatgc cgcgctaaca agggtgaagc tcagcttgac cctcatatag acggcgcccc
     1261 tggggaggta agcggcgtca cgacccttca gcaaggagaa ggaccgtggc tctatctgcc
     1321 cctacccagt atcaggctaa cgctcaagct gagcttgatg atcactacct ggtcgccctc
     1381 gccaaggacg gccgccccga cgcttacgac atgatcgtca agcgttaccg cgggttcgtc
     1441 cgcctcaagg cctcttcgta cttcctgctc ggcggcgagt ccgacgacct gatccaggag
     1501 ggcctgctcg gcctctacaa ggcgatccgc gactaccgga ccgaccgcga gtcgagcttc
     1561 cgcaacttcg ccgagctctg catcacccgc cagatcatca ccgcggtgaa gaccgcgacc
     1621 cgcaacaagc acacgccgct gaaccagtac gtctccttca gccagacccc ggccgcggcc
     1681 ggcgactccg acacgacgct cgacgagatc ctgcccgggc cgtccgtcca cgacccggtc
     1741 aaccaggtga tcgccaccga agagctggag agcctcgtct cctgtctctc cggcgtctcc
     1801 ggggtgctct cggacctcga gagccgcgtg ctctcgctct acctcgacgg tcactcctac
     1861 gaggcgatcg ccgagcggct ggagtgcgac acgaagacgg tcgacaacgc cctgcagcgg
     1921 gtcaagcgca aggtgggttc gcacctcgcc tcgcgcgagg tagcgctcta ggcgactttc
     1981 tccgccggta gatcggcgag gacgtactcg agtagctccc gcccgtggtc cgaggcgacg
     2041 gcaacgtcgt cgaccttgac tcggacccgg tgcagggact gcatgcgcag cacgtcgccg
     2101 tcggtgcggt ggttggggaa gaccccggcg taggagatcc ccagccgctc caggtcctcg
     2161 gcgaccagcg ccgtcgccgg cgtctccagc ggcaggtcga agtagaccgc gtcgagcttg
     2221 cgggcgtgga agaggtggtg gcgctcggcc cgcaggacct cggcgaggtc ggcgccgggc
     2281 acggtgacgg tgatcagcgc caggttgtgg tcgtcgtcga cgtcgacctg gaaccgggtg
     2341 cgctccggca gatcgaaccc ggccggaggg tcggccagct cgccgcgcag ctcgcagagg
     2401 gcgagggtct ggccgacgat ctcgcggtgg cgggcgggcg cgtagacctg gcgttcgtgg
     2461 ccgtcgttca gcttcgagta gaagagagcc gccgactggc gccggcgcgg cgccggggcg
     2521 gcggcgtcgt tggcgaccgt ggccgggatc cagccgagca ggaagccggt ctcgtgggcg
     2581 ccgagctcga cgttcgcctt ctggctgtag gggtgggcgg cggtcgcctc gctgaacatc
     2641 cccgccagcc cctcgccctg cgcccagtcc atcaggtgcc gcttggtgcg ggtgaacatg
     2701 tgctggccac gggcgagcgg catcgtcacc gcctggccgg agtgggcgac cgcgtcgccg
     2761 gccgccgcca ggctcatgcc ctcatggcag agcagctcgc cgtcgggggt ctcggcgacg
     2821 caggagacgt agcgcccgtc gcccagccgc gccgccacct cgttcgggtc gtagacccag
     2881 cgcacgtcgt aggtccgccc gtaggccgcc tcgatcgcgc cggtcagcac atgcccttca
     2941 tcggcgctca agaacctgaa aaccgcatcc atctgtctat attcggccaa gcgcccccct
     3001 agctcaacct gggagagcac ccgatttgta atcgggaggt ttccggttcg aacccggagg
     3061 ggggctctca cgccccgcgg cgtgggaagc ccttcggggc gcggcggccg gttgccgccg
     3121 cgcctccgta gccgggtcgg ctagccgccg atccgcgagc agaccacgac gatcttggct
     3181 ttggtgacgt tcgccgcgcc accctgggtg gtcatcgccc cgtaccagcc ggtcggcggg
     3241 ccgtgctcgc cgtcggcgac cgcctcggtg cgggcgccat cgatggtcgg ggcggaggca
     3301 gagaccttgc cgttgaggat gccgtcattg tcgacgccgc cggcgatcgc ttggtagccg
     3361 gccgggcact cgacgatgcc gctctcgaag ccgccggggg gagcgatgtt ggtgccgatg
     3421 gtctcgacca cggtcaccgt cgcctgatgc agataccgcg cgtcgctctc gcctttggtg
     3481 taggcgttga cgggagccgg gatcgtcccg gccttgaggt cggtggtgtc gatcgagccg
     3541 gctgccacct tggcgccgtt gactgcgtcg ttcttgatct tcgcggtggt cacggcttcc
     3601 ttcttgagct tcgccgtggt caccgcgttg ttcttgatct gtttggcggt gacgctgttt
     3661 ttcggcagct gggtggccgc gaaggcgctg gcgccgccga cggcgatgca gagggcgagc
     3721 gtggcgacca cgttggcgta ggtcagccgc cccttgaaga gcttcttcat ccgagtcctc
     3781 ctggagattg gcagtgcgag acgcgcgcac tctaggcacc ggggcggcga gaaactgcgc
     3841 cgtaagcggg tgtcctactc gccgccgagg acgcgcgcct tctcggcggc gaactcctcg
     3901 tcggtgagga tgcccttctc gcgcagctcg ccgagccgct cgagccgggc gaggcgctgg
     3961 tcctcgccga gcacggccgt cggcgcctcg gtggtggggg cctcgctcgt cggcgtctca
     4021 ccgccgtcgg agcgcggcac ctcgggcagg cgcacttttt tcgcgctctc gcccagtttc
     4081 gccgcccgct cgccgaggtt ggcgcctttg accgcgctca cgacccggtc gcgcggcccg
     4141 ccgaaggtgt cgctgaactt ggcgtcgggg aactcctcga tgcactgttt gcgcagctcg
     4201 tggatccagg ctgc
//
LOCUS       DASRIG010000276         7589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3801976, whole genome shotgun sequence.
ACCESSION   DASRIG010000276 DASRIG010000000
VERSION     DASRIG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801976"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VFB52_07600"
     CDS             <1..1577
                     /locus_tag="VFB52_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO06236.1"
                     /translation="RYREELRLHDDRHEAMAEALRRAGPAILASGMTVIAALLCLLIA
                     KVNGTSGLGPIAAIGIACAALSMLTLLPALLTIFGRRAFWPFVPHTPATAPAPTAIPE
                     WSWRNIVEGSGPKAIGLVVLGCLLVVFPPFLVVILLNWLLRCLNPLLRRVIGKEIPSL
                     FAFTLDRAFFKPFELRRFQREHAADETHGFWSRLGKRIAVSPKRIMSGTIVVLLILCA
                     GLATFSTDLTSEDTYREEVESVEGQDLLNLSFPSGTTAPTDIVVVDPNKIPAVAEAVE
                     GVAGVEAVGPPIANGPPGTLIQATLEPNPYSTEAFDLIDPIRDAAHGAEPEALVGGPT
                     AVEFDVRDAAGWDSIVIPPIILLVVFLILMGLLRAVIAALVLIGTVILSFLAALGVGY
                     FFFDFVFDFPGSDPSLPLFAFVFLVALGVDYNIFLIARAREETEKHGAEQGILRALAV
                     TGGVITSAGIVLAGTFAVLGVLPLTFLTELGFVVAFGVLLDTFVVRSILVPAIALTLG
                     DKFWWPSALSHGRSSD"
     gene            complement(1612..2289)
                     /locus_tag="VFB52_07605"
     CDS             complement(1612..2289)
                     /locus_tag="VFB52_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZO06237.1"
                     /translation="MATLHVLRVFTGEDGAHGNPLGVFLDGAEVPAERRQAVAHELGF
                     SETVFVDDRSRGECRIFTPGLELPFAGHPTVGTAWLLAHEGDAGEALNPPAGEVAVHR
                     EGELTYVSARADWSPPWELIEYGSPAEIDALDGSPDGREDEIYLWAWEDEAAGTVRSR
                     CFSLADGVGEDEATGSAAIMMAAEQNRPLTIHQGEGSLLHAAPLGDGWAEVGGRVVLD
                     ERRDFAG"
     gene            complement(2305..3657)
                     /locus_tag="VFB52_07610"
     CDS             complement(2305..3657)
                     /locus_tag="VFB52_07610"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZO06238.1"
                     /translation="MAEQPRSAADVIELVKQNDVRFIRLWFTDVLGQLKSFSVNASEL
                     EDAFEGGMGFDGSSITGFNPIEESDMLAVPDPATFELIPWRPDDEDLVARMFCDIRVP
                     GGGPYEGDPRWIMRRALKRAEEMGFEAYNIGPELEFFYFRSAKPENGVPEPLDEGGYF
                     DLTTLDAGSDLRRETVLALEKMGIHVEYTHHEVGPSQHEVDMRYKNALEMADDCMTYR
                     IVVKEYALKYGYHATFMPKPLFGENGSGMHVHQSLSKDGKNAFYDEDDPYYLSPVAKS
                     FIAGQLKHAREISPLFAQWVNSYKRLVPGYEAPVYLAWSRRNRSALIRVPLYHPGKEA
                     ATRAELRCPDPAANPYLCFAGMLQAGLEGVEKGYELPEPMEQNLYHLSQEELAHQGIE
                     QLPATLGEAIEIAADSELVLRTLGEHTFRRFVEIKRQEWDEYRVQVTPFEIENFLPIL
                     "
     gene            3920..4486
                     /locus_tag="VFB52_07615"
     CDS             3920..4486
                     /locus_tag="VFB52_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO06239.1"
                     /translation="MTVKTAVSRFQIHDELTAPDESAPLIKTIQATGGMVTKFVGVLA
                     GSPAVLRAYTRMRSELRGGVLPQATRERIALAVAEHREDNYSIAQHAKSARRAGLGLD
                     EVSRARSFTSADEKEAALLLFLKEALDADGPPPLHLHEEAREAGWTDEEILEAVGHLA
                     LNEFQSLIASAAALPKDQATPRVLPSAA"
     gene            complement(4549..5268)
                     /locus_tag="VFB52_07620"
     CDS             complement(4549..5268)
                     /locus_tag="VFB52_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06240.1"
                     /translation="MSRSHQGQSTKRRRGRWAVAAVVGCSLALVAAFAGSTSAAKVEK
                     TTVILGQTSTTPDPACPGEPCQAIGSVTGFQVNNGQTVNPFLVKTNGTIKSWTLTLAQ
                     PTNSQRNFFNGFFGTPPEARLSIIRRVPATNPPRYTLRRQGAIKVLSPYLGQTVKFGS
                     NLKVEKGDIVAISVPTWAPAFSQDLPKNNVWRASRAPGTCSNSTDIRQGEPQSVVGKR
                     ATYGCKYTTARLLYTATLVEG"
     gene            complement(5377..5826)
                     /locus_tag="VFB52_07625"
     CDS             complement(5377..5826)
                     /locus_tag="VFB52_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZO06241.1"
                     /translation="MLRWALVLAALVIAALGPAAAQAGTGGASSYTAPKPSAASTSAG
                     QYTFSAWKSAGASWYGPGLYGNGVACGGTLRPSTVGVAHKTLPCGTMVKFKHRGHVVM
                     AKVIDRGPYIAGRTWDLTEAAAEALDFEDVGVGRVQYAVATSYAKTK"
     regulatory      complement(5846..5993)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(6101..6436)
                     /gene="rnpB"
                     /locus_tag="VFB52_07630"
     ncRNA           complement(6101..6436)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB52_07630"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6494..7327
                     /locus_tag="VFB52_07635"
     CDS             6494..7327
                     /locus_tag="VFB52_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06242.1"
                     /translation="MRNAVVTGAGSGLGREIARELAGRGYRVHATDLDPGSAAATAAE
                     LGGEAGSSALDVRDENACRVLADQIAADGGLELWVNNAGVFFSGEPWEQGAELRRLML
                     EVNTNGLINGTLAALVPMKATGRGHVINVVSLAGIVAAPGEVNYSASKHAAMAFTIGT
                     QFDLRLAGIGGVRVSAVCPDGIWTPMLEDKLDDPHAAGSFSGQLQTAEQVAHAVGKLS
                     AKPRPVTIVPRWRGPQLRLLDAFPGLALRMLPLIMWDARRRQKRYKKLIAAGKWPPRG
                     R"
     gene            complement(7329..>7589)
                     /locus_tag="VFB52_07640"
     CDS             complement(7329..>7589)
                     /locus_tag="VFB52_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HZO06243.1"
                     /translation="DYILQVRGESMKNAGIIEADYVIVRPADDAADGEIVVALIGEEA
                     TVKRFFREADHIRLQPENDEMEPILTTEARVLGKVVGVFRKV"
BASE COUNT         1124 a         2620 c         2559 g         1286 t
ORIGIN      
        1 ctcgataccg ggaggagctg cgccttcacg acgacagaca cgaggcgatg gccgaagcgc
       61 tccgccgggc cgggccggcg atcctcgcct cggggatgac cgtgatcgcg gcgctgctct
      121 gcctgctgat cgccaaggtc aacggcacct ctggcctcgg cccgatcgcc gcgatcggga
      181 tcgcctgcgc ggcgctctcg atgctgaccc tgctgccggc gttgctgacg atcttcggcc
      241 gccgcgcctt ctggcccttc gtcccgcaca cgccggcgac cgcaccggcg ccgaccgcga
      301 tcccggagtg gtcgtggcgc aacatcgtcg agggcagcgg gccgaaggcg attgggctcg
      361 tcgtgctcgg ctgcctgctc gtcgtcttcc cccccttcct cgtcgtgatc ctgctcaact
      421 ggctgctgcg ctgtctcaac ccgctgctgc ggcgggtcat cggcaaggag atcccgtcgc
      481 tgttcgcctt caccctcgat cgcgcgttct tcaagccgtt cgagctgcgc cgcttccagc
      541 gcgagcacgc cgccgacgag acgcacggct tctggtcgcg gctcggcaaa cgcatcgcgg
      601 tctcgccgaa gcggatcatg tcgggcacga tcgtcgtcct cctcatcctc tgcgccgggc
      661 tcgccacctt ctccaccgac ctgaccagcg aagacaccta ccgcgaagag gtcgaatcgg
      721 tcgaaggcca ggacctgctc aacctcagct tcccctcggg aaccacggcg ccaaccgaca
      781 tcgtcgtcgt cgacccgaac aagatcccgg cggtggcgga agcggtcgaa ggcgtggccg
      841 gcgtcgaggc ggtcggcccg ccgatcgcca acggcccgcc cggcaccctg atccaggcga
      901 cgctggaacc gaacccgtac tcgaccgagg ccttcgacct gatcgatccg atccgcgacg
      961 ccgcgcacgg cgccgaaccc gaggccctgg tcggcggccc gaccgcggtc gaattcgacg
     1021 tccgcgacgc cgccggctgg gactcgatcg tgatcccgcc gatcatcctg ctcgtcgtct
     1081 tcctgatcct gatggggctg ctgcgcgcgg tgatcgcggc gctggtcctg atcgggaccg
     1141 tgatcctcag cttcctggcc gcgctcggcg tcggctactt cttcttcgac ttcgtcttcg
     1201 acttccccgg ctcggacccg tcgttacccc tgttcgcctt cgtcttcctg gtcgcgctcg
     1261 gcgtcgacta caacatcttc ctgatcgccc gtgcccgcga ggagaccgaa aagcacggcg
     1321 ccgaacaggg catcctgcgg gcgctcgcgg tcaccggcgg cgtgatcacc agcgccggga
     1381 tcgtgcttgc cggcaccttc gcggtgctcg gcgtgctgcc gctcaccttc ctcaccgagc
     1441 tcggcttcgt cgtcgccttc ggcgtcctgc tcgacacctt cgtcgtccgc agcatcctgg
     1501 tgccggcgat cgccctgacc ctgggcgaca agttctggtg gccgtcggcg ctgagccacg
     1561 gccgctcgtc agattgaccc gtccctggtc gggtcaatac gtcgagcgcg cttagcccgc
     1621 gaaatcgcgg cgctcgtcga ggacgacgcg gccgccgacc tccgcccaac cgtcaccgag
     1681 gggcgcggcg tgcagcagcg agccctcgcc ctggtggatc gtcagcggcc gattctgctc
     1741 ggcggccatc atgatcgccg cggagcccgt cgcctcgtcc tcccccacgc cgtcggcaag
     1801 cgagaagcag cgcgagcgga ccgtgccggc cgcctcgtcc tcccaggccc ataaatagat
     1861 ttcgtcttcg cggccgtccg gcgacccgtc gagcgcgtcg atctcggccg gcgagccgta
     1921 ctcgatcagc tcccacggcg gcgaccagtc ggcgcgggcg gagacgtagg tcagctcgcc
     1981 ctcacggtgc accgccacct cgccggcggg cgggttcagc gcctcgcccg catcgccctc
     2041 gtgagcgagc agccaggccg tgccgacggt cgggtgaccg gcgaagggca gctccagccc
     2101 cggggtgaag atccggcact cgccgcgcga gcggtcgtcg acgaagaccg tctccgagaa
     2161 gccgagctcg tgcgcgaccg cctgccggcg ctcggccggc acctcggcgc cgtcgaggaa
     2221 gaccccgagc gggttgccgt gggcgccgtc ctcgccggtg aagacgcgca ggacgtgcag
     2281 cgtcgccaca gccgtcctcg ctccctagag gatcgggagg aagttttcga tctcgaacgg
     2341 ggtgacctgg acccggtact cgtcccattc ctgccgcttg atctcgacga agcggcggaa
     2401 ggtgtgctcg cccagggtgc gcaggaccag ctccgagtcg gcggcgatct cgatcgcctc
     2461 gccgagcgtc gccggcagct gctcgatccc ctggtgggcc agctcctcct gagagaggtg
     2521 gtagaggttc tgctccatcg gctcgggcag ctcgtagccc ttctcgaccc cttccaggcc
     2581 cgcctgcagc atcccggcga agcacaggta cgggttggcg gccgggtccg ggcaacgcag
     2641 ctcggcgcgg gtcgcggcct ccttgccggg gtggtagagc ggcacccgga tcagcgccga
     2701 gcggttgcgg cgcgaccagg cgaggtagac cggcgcctcg tagccgggca ccaggcgctt
     2761 gtaggagttg acccactggg cgaacagcgg cgagatctcg cgcgcgtgct tgagctggcc
     2821 ggcgatgaac gacttcgcca ccggcgagag gtagtacggg tcgtcctcgt cgtagaaggc
     2881 gttcttgccg tctttggaga gcgactggtg gacgtgcatc ccggatccgt tctcgccgaa
     2941 cagcggcttc ggcatgaagg tcgcgtggta gccgtatttg agcgcgtact ccttgaccac
     3001 gatccggtac gtcatgcagt cgtcggccat ctcgagcgcg ttcttgtaac gcatgtcgac
     3061 ctcgtgctgg gagggcccga cctcgtggtg ggtgtactcg acgtggatgc ccatcttctc
     3121 cagcgccagc acggtctcgc ggcgcaggtc ggagccggcg tcgagcgtgg tcaggtcgaa
     3181 gtagccgccc tcgtcgagcg gctcggggac gccgttctcg ggcttcgccg agcggaagta
     3241 gaagaactcg agctcggggc caatgttgta ggcctcgaag cccatctcct cggcccgctt
     3301 cagcgcccgt cgcatgatcc agcgcgggtc gccctcgtag gggccgccgc cgggcacacg
     3361 gatgtcgcag aacatgcgcg cgaccaggtc ctcgtcgtcg gggcgccagg ggatcagctc
     3421 gaaggtcgcg gggtccggca ccgccagcat gtcggactcc tcgatcgggt tgaagccggt
     3481 gatcgaggat ccgtcgaagc ccatcccgcc ttcgaaggcg tcctcgagct cggaggcgtt
     3541 gaccgagaag ctcttcagct gcccgagcac gtcggtgaac cagaggcgga tgaagcgcac
     3601 gtcgttctgc ttgaccagct cgatgacgtc agcggcactg cggggttgct cagccattca
     3661 ctgcctcctt ggctctgggc gcgagaaggt cgcaacgata ggcgaatgaa ccggatgagt
     3721 gcataacgcc gtattccggg tatggaaagg cagtttgtct cgacagagaa aggcaaagtg
     3781 acccggcttg cacgagccgc cgaaagcagg tataacttac ttcacgtaag taagaggctc
     3841 aaagtaaagt aaaggccacg taaaggctct gtaaagcgaa attgtcgctc ccagaaagag
     3901 ccagaagcga gacgcaacag tgacggtaaa aaccgcagtc agcagattcc agatccacga
     3961 cgagctcacg gcgcccgacg agagcgcgcc gctgatcaaa accattcagg cgaccggcgg
     4021 catggtcacc aagttcgtcg gcgtgctcgc cggttcaccg gccgtcctgc gcgcctacac
     4081 ccggatgcgc tccgagcttc gcggcggcgt ccttccccag gcgacccgtg agcggatcgc
     4141 cctggcggtc gccgagcacc gcgaagacaa ctacagcatc gcccagcacg ccaaatcggc
     4201 ccgccgggcg ggtctcggcc tcgacgaggt ctcccgcgcc cgcagcttca cctcggcgga
     4261 cgagaaggag gcagcgctgc tgctcttcct caaagaggcg ctcgacgccg atggccctcc
     4321 cccgctccac ctccatgagg aggcgcgtga agcgggctgg accgacgagg agatcctcga
     4381 ggcggtcggc cacctggcgc tcaacgagtt ccagagcctg atcgccagcg ccgcggcgct
     4441 gcccaaagac caggcgaccc cgcgggttct cccctcggcc gcctagtcca cagacgtaac
     4501 tcctgagtat ccggggaggg gcgcaagccc ctccccttct tcgtggccct agccctcgac
     4561 cagggtcgcg gtgtagagca ggcgggccgt cgtgtacttg cagccgtagg tggcgcgttt
     4621 gccgaccacg ctctgcggct caccctggcg gatgtcggtc gagttggagc aggtgccggg
     4681 ggcgcggctg gcgcgccaga cgttgttttt cggcaggtcc tgcgagaagg ccggggccca
     4741 ggtcggcacg gagatcgcga cgatgtcgcc cttttcgact ttcaggttgg agccgaactt
     4801 gaccgtctgg ccgaggtagg ggctgagcac tttgatcgcg ccctggcgcc gcagggtgta
     4861 gcgcggcggg ttggtggcgg gaacgcggcg gatgatcgag aggcgggcct ccggcggggt
     4921 gccgaagaag ccgttgaaga aatttcgctg gctgttggtc ggctgcgcca gggtcagcgt
     4981 ccacgacttg atcgtgccgt tggttttgac caggaacggg ttgaccgtct ggccgttgtt
     5041 gacctggaag ccggtgacgc tgccgatcgc ctggcacggt tcgcccgggc aggcggggtc
     5101 cggcgtggtc gacgtctgac cgaggatcac cgtcgtcttc tccaccttgg cggcgctggt
     5161 gctgccggcg aaagcggcga cgagcgcgag cgagcagccg accacggctg caacggccca
     5221 gcggccgcgg cggcgcttgg tggactgacc ttggtgggac ctcgacataa ctgctgctcc
     5281 tctcggtatt tggccctgcc taaacacggc agattagcct tcggagcggc agttttacgg
     5341 tatttgcggg tcccggcgcc tcgccggtac gtggcgctac ttcgttttcg cgtagctggt
     5401 cgcgaccgcg tactggacgc gcccgacacc gacgtcctcg aaatcgagcg cttcggcagc
     5461 ggcctcggtc agatcccagg tgcggccggc gatgtagggg ccgcggtcga tcaccttggc
     5521 catcacgacg tggccgcggt gtttgaactt gaccatggtc ccgcagggca gcgtcttgtg
     5581 ggcgacgccg acggtcgagg gccgcagggt gccgccacag gcgacgccgt tgccgtagag
     5641 gcccggcccg taccagctcg cgccggcgct cttccaggcg ctgaaggtgt actggccagc
     5701 cgaagtgctg gcggcgctcg gcttcggcgc ggtgtagctg gaggcgccac cggtgccggc
     5761 ctgcgcggcg gcagggccga gagcggcgat cacgagggcg gccaggacga gagcccagcg
     5821 cagcatgggt tttctgcggt ttctcaattt ctctttccag gcgcttacgg ggttagctgt
     5881 cgggctcgcg ctgaaagagc tgcgctacgc cggaccaggc cggcgactcg ccccaactaa
     5941 ttgggtcccc cgctccccgc ggtatgtagg cggagattca gcggaagttg gcggcagatt
     6001 agcaaacgct aagacggaaa gttggatttc gttagcgaac cttcatagaa atctcgcaat
     6061 ttggccctgg aatgggaaag gcccccatcg ctgggggcct tcctttagcg ggcctgtaag
     6121 ccggatcctg tcttgagcgg ccatctatct ttgcggccta cctggacctc gccgagccgg
     6181 gtcgacggtc ctgcttggcc ttgcaccggg cggggtttgc ctggccgccg cgtcaccgcg
     6241 acgccggtgc gctcttaccg caccttttca cctttgcctg tacgcgactc agctgaggcc
     6301 gcgccatcgg ccgtgtgttt ctgtggcact ttcccgcggg tttccccggg tgggcgttac
     6361 ccaccgccct gccctgtggt gtccggactt tcctcgaggg gttttctctc cccccgcggt
     6421 ccgctcggcc cgcccaccga tggtagcccc ggtccgagcc cggggcaagc tcgcgcgggt
     6481 tatggtgggc gcgatgcgaa acgcggtggt cacgggggcg ggaagcgggc tggggcgcga
     6541 gatcgcccgt gagctcgccg ggcgcggcta ccgcgtccac gccaccgacc tcgaccccgg
     6601 gagcgccgcc gcaaccgcgg ccgagctcgg cggcgaagcc ggctcctccg ccctcgacgt
     6661 gcgcgatgaa aacgcctgcc gggtgctcgc cgaccagatc gccgccgacg gcgggctcga
     6721 gctctgggtc aacaacgccg gcgtcttctt cagcggcgag ccctgggagc agggcgccga
     6781 gctgcgtcgg ctgatgctgg aggtgaacac gaacgggctg atcaacggca ccctggcggc
     6841 gctggtaccg atgaaggcga ccggccgcgg ccacgtgatc aacgtcgtct cgctggccgg
     6901 gatcgtcgcc gcccccggcg aggtcaacta ctcggcgagc aagcacgcgg cgatggcgtt
     6961 cacgatcggc acccagttcg acctgcgcct cgccgggatc ggcggcgtcc gcgtctccgc
     7021 cgtctgcccg gacggaatct ggacgccgat gctggaagac aagctcgacg acccccacgc
     7081 tgccggctcc ttctccggcc agctgcagac cgccgagcag gtcgcccacg cggtcggcaa
     7141 gctcagcgcc aagccccgcc cggtcacgat cgtgccccgc tggcgcggcc cgcagctccg
     7201 cttactcgac gccttccccg gcctcgccct gcgcatgctg ccactgatca tgtgggacgc
     7261 ccgcaggcgc cagaagcgct acaagaagct gatcgcggca ggcaagtggc cgccgcgcgg
     7321 ccgctagctc agactttgcg gaagaccccg accactttgc cgaggacacg cgcctcggtg
     7381 gtcaggatcg gctccatttc gtcgttctcg ggctggagcc ggatgtggtc ggcctcgcgg
     7441 aagaaacgct tcacggttgc ctcttcgccg atcagggcga cgacgatttc gccgtcggcc
     7501 gcgtcatcgg ctgggcgcac gatcacgtag tcggcttcga tgatgccggc gttcttcatg
     7561 ctttcgccgc gcacctggag gatgtagtc
//
LOCUS       DASRIG010000277         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2465285, whole genome shotgun sequence.
ACCESSION   DASRIG010000277 DASRIG010000000
VERSION     DASRIG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465285"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFB52_07645"
     CDS             complement(<1..674)
                     /locus_tag="VFB52_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06244.1"
                     /translation="MGLLDGKAAIVTGSARGIGRATAELFAGEGAKVLINDLDGDVAE
                     QAASEISGETAVFSGDLTKPGACDEMVAAGVDAFGAVDIVVNNAGYTWDGVAHKMSDE
                     QFQAMLDIHTIVPFRVARALAPHWREAAKAERGEDKEVFRKIINISSTSGTMGNPGQV
                     NYSAAKMGVVGVTKTLAKEWGQFKINVNAVAFGFVDTRLTAAKEEGGEITSPTGEKIE
                     LGIPEQ"
     gene            925..1635
                     /locus_tag="VFB52_07650"
     CDS             925..1635
                     /locus_tag="VFB52_07650"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HZO06245.1"
                     /translation="MDWGSFLLLALLAMFTPTLLAAVTIMMLLPNPRRLMIGYLLGAY
                     MTSITLGLVIVFSLHNSEIVGTGQRTLGPGEDIVLGLLLLVIAYVLHSDRDARLRERR
                     QRHKEAKEAKKAAGDAKPSWPERMLGKGDPRVTFAVGAALTLPGVSYLTALDRIADYD
                     PGPFPTVLLVVGFCLVQLTFIELPLLGYAFAPETTQDRVTRFRAWLSRNGRRVAIRVA
                     AVLGGLLILRGILEFIFA"
     gene            complement(1643..1945)
                     /locus_tag="VFB52_07655"
     CDS             complement(1643..1945)
                     /locus_tag="VFB52_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06246.1"
                     /translation="MLCALTVRKLKPGTIEDFKAAFVPSPEMEPPPGWKRFYAIRGVT
                     DENEIVTFGFFDGTLEELRSNQADNDYDERRAAAEQYVESVGTDGVFEVVDERVIG"
     gene            2090..4384
                     /locus_tag="VFB52_07660"
     CDS             2090..4384
                     /locus_tag="VFB52_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO06247.1"
                     /translation="MREPKDRNWPEHEVDEFLDQISARGDDYRHLVERLPVIVYAAEL
                     GEHGKWRYVSPQVEEILGYSAEEFKRDNGLWARLLHPDDREAALDLEKEHWLGNRNTS
                     PTVYRMRTRAGETVWMLDEAVLEKDDEGIPVWHGVLYDITESKTAEAELQRALAQQAV
                     VAKLGERALKDGDPEKLMQAAVSLISEVDGIHSACIWEVGRDGRRLHLSAGLEDEVVG
                     ASRRVSAARDSHAGTALDTGKPAIVRDWPSEERFTMPPILRVFGANSSLAVIIDGKDR
                     PYGVLDVHATVPGRFTAKDVPFVQGAANVLADAFERHAADQALRHRVLHDSLTGLPNR
                     LSFVDALGEALRRAEVSGSPVGILFLDLDHFKLINDSLGHHAGDALLRAVAPRLRGHL
                     RPGDIVARFGGDEFGILIDRLADEGEAVAIADRVSAAFTQPFAIDGVEHFVSSSIGVA
                     VARSSERQVNAELLIRDADAAMYRAKESGRARCVLFDAEMRAGAMRRLEVERELRTAL
                     DRDELALHYQPVVSLGTGEITGLEALVRWQHPERGLLDPAEFVSIAEDSGLIEPLGRW
                     VQERACQQAVEWQNERPDARPFDVAVNLSARQVAHRDLPATVAEIIARTGLDPVHLRL
                     EITESVLVEESASAISSLEALNEIGVRLVLDDFGTGYSSLAYLNRYPFHALKIDRSFV
                     DALGIEQERTAIVEAIIGMARALSLEVIAEGVENEVQLSELRRLGCDYAQGHLFHAAM
                     PDTQVSRMLEEGALGYSAGISPGK"
     gene            complement(4354..5379)
                     /locus_tag="VFB52_07665"
     CDS             complement(4354..5379)
                     /locus_tag="VFB52_07665"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO06248.1"
                     /translation="MKTPRLVAIVTALAILLALTLAACGEKSEDSSGADSQSLSLALD
                     FYPNPDHAGIYMAEKLGYFEEAGLEVGIEAPTDPSLPIKQVAAGRTDLAISYEPEVIL
                     AHERGLDVVAIGALVNEPLTSMIWLQKSGIKAVPGLKGKTIAYAGIPYQEAFLETILT
                     RAGLTTSDVKAVNVGFNLLPALVGGSADAMLGGFRNVEGVDLRERGLKPVVTPVDQLG
                     VPTYDELVLVAKRETVEAEPEKFRLFMAALERGTNAAVAQPAAATEAILAANDALEPK
                     LTRAEVEATLPLLGARTEGQPYGYMDPTAWDEFAGWMRDNELIESLPSAAELLSNDYL
                     PGEIPAE"
     gene            complement(5390..6142)
                     /locus_tag="VFB52_07670"
     CDS             complement(5390..6142)
                     /locus_tag="VFB52_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06249.1"
                     /translation="MKRWLYPLALLAALIGLWQLAASTGFLAEVMNLEDFLVPSPAEI
                     ASQLWEERTLLLENGWVTLQEIVLGFLLAVVVGVAFAVAMHFSTTVRLAANPLVIVSQ
                     TIPVIVIAPIFVIWFGFGITPKLIIIALICFFPVTVATLDGLRSADPAAIKLMRTLYA
                     SRMQTFRRVESRGALPSFFSGARIAAAIAPIGAVFAEWAGANEGLGRLILISNANFQV
                     ALTFAALVVLSAIAIALYALVALAERRIITWR"
     gene            complement(6139..>6533)
                     /locus_tag="VFB52_07675"
     CDS             complement(6139..>6533)
                     /locus_tag="VFB52_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06250.1"
                     /translation="AGFEAMKPGELSGGMRQRVAFLRTLVSGKPVLALDEPFAALDAI
                     TRAEMQEWLASALRTDPRTVVLVTHDVEEALYLADRVAVLSARPARIVAELDAPDPRA
                     ADRDAAVTDPAFVAARERALRALHEGSR"
BASE COUNT          990 a         2271 c         2247 g         1025 t
ORIGIN      
        1 atctgctcgg ggatgccgag ctcgatcttc tcgccggtcg gcgaggtgat ctcgccaccc
       61 tcctctttgg cggcggtgag gcgggtgtcg acgaagccga aggcgaccgc gttgacgttg
      121 atcttgaact ggccccactc cttggccagg gttttggtca ccccgacgac gcccatcttg
      181 gccgccgagt agttgacctg gcccgggttg cccatcgtcc ccgaggtcga ggagatgttg
      241 atgatcttgc ggaagacctc tttgtcttcg ccgcgctcgg ccttcgccgc ctcacgccag
      301 tgcggggcga gcgcccgggc gacccggaac ggcacgatcg tgtggatgtc gagcatcgcc
      361 tggaactgct cgtcgctcat cttgtgggcg acgccgtccc aggtgtagcc ggcgttgttg
      421 acgacgatgt cgaccgcgcc gaaggcatcg accccggcgg cgaccatctc gtcgcaggcg
      481 ccgggcttgg tcaggtcgcc ggagaagacc gcggtctcgc cggagatctc gcttgccgcc
      541 tgctcggcga cgtccccgtc gaggtcgttg atcagcacct tggcgccctc gcccgcaaac
      601 agctcggcgg tcgcccggcc gattccgcgc gccgatccgg tgacgatcgc cgctttaccg
      661 tccagaagtc ccatcgctcg ttcccattcc tttcgtgcgt gaaagttgcc cgcgcaggct
      721 acacggatca gcgcagcata gtcatgtttg gctgactagc cagtcagccg cttctacagg
      781 cttacggagc cgtcgtcatt gacctgtcag tgggcgttgt gggcgatctc ccagggggtg
      841 gccgtcgggg tcgatggaga cgccgagggt gatcaggctt gactcgttgc ttcacgacgc
      901 cgatccttcc aggagaatgg tcatgtggac tgggggagct tcctactgct cgcgctgctg
      961 gccatgttca cgccgaccct tttggcggcg gtgacgatca tgatgctgct gccgaacccg
     1021 agacggctga tgatcggcta cctgctcggg gcctacatga ccagcatcac cctgggcctg
     1081 gtgatcgtct tctcgctgca caacagcgaa attgtcggca ccggccagcg caccctcggg
     1141 cccggcgagg acatcgtcct cggcctgctc ctgctggtga tcgcctacgt cctccacagc
     1201 gaccgcgacg cgcgcctgcg ggaacggcga cagcggcaca aagaagcgaa ggaggccaaa
     1261 aaggccgccg gcgacgcgaa accgtcctgg ccggagcgga tgctgggcaa gggcgacccg
     1321 cgggtcacct tcgcggtcgg cgccgccctc accctgcccg gcgtctccta tctgaccgcg
     1381 ctcgatcgga tcgccgacta cgaccccggc ccgttcccaa ccgtgctcct cgtcgtcggc
     1441 ttctgcctcg tccagctcac cttcatcgag ctgccgctgc tcggctacgc cttcgcgccg
     1501 gagacgaccc aggatcgggt cacccgcttc cgcgcctggc tctcccgcaa cggccgccgg
     1561 gtcgcgatcc gcgtcgccgc cgtgctcggc gggctgctga tcctgcgcgg gatcctcgag
     1621 ttcatcttcg cctagccgcc gctcagccga tcacccgctc gtcgaccacc tcgaagacgc
     1681 cgtcggtgcc gaccgactcg acgtactgct cggccgccgc gcggcgctcg tcgtagtcgt
     1741 tgtcggcctg gttggagcgc agttcctcga gcgtgccgtc gaagaacccg aaggtgacga
     1801 tctcgttctc gtcggtgacg ccgcggatcg cgtagaagcg cttccagccg ggcggcggct
     1861 ccatctccgg cgagggcacg aaggcggctt tgaagtcctc gatcgtgccc ggtttgagct
     1921 tgcggacggt cagtgcgcag agcattggat ctcctctcct catgcacctg gccgagctgc
     1981 ccaaatgggc ctcagtcgat cctaacccga cctcagccag gcctgcacga actggtgcct
     2041 ggacaggcag gggtcaagcc tgagccgtgc ctgcccgata gagacgcaga tgagggagcc
     2101 caaagacagg aattggcccg agcacgaggt cgacgagttc ctcgatcaga tttcggcgcg
     2161 gggcgacgat taccgccatc tggtcgagcg tctgcccgtg atcgtctacg ccgccgagct
     2221 cggcgagcac ggcaagtggc gctacgtcag ccctcaggtc gaggagatcc tcggctactc
     2281 cgcggaggag ttcaagcgcg acaacggcct ctgggcgcgc ctgctgcatc ccgacgaccg
     2341 tgaagcggcg ctcgacctcg agaaagagca ctggctcggc aaccgcaaca ccagcccgac
     2401 cgtctaccgg atgcgcaccc gcgccggcga gaccgtgtgg atgctcgacg aggcggtgct
     2461 ggagaaagac gacgagggga tcccggtctg gcacggggtg ctctacgaca tcaccgagag
     2521 caaaaccgcc gaggcggagc tgcagcgggc gctcgcccag caggcagttg tcgccaagct
     2581 cggcgagcgg gcgctgaagg acggcgaccc ggagaagctg atgcaggccg cggtcagcct
     2641 gatctccgaa gtcgacggca tccacagcgc ctgcatctgg gaggtcggcc gcgacggccg
     2701 ccgcctgcat ctgagcgccg ggctcgagga tgaggtcgtc ggcgccagtc gccgcgtctc
     2761 cgccgcccgc gactcgcacg ccggcaccgc gctcgacacc ggcaagccgg cgatcgtgcg
     2821 ggactggccc agcgaggagc gcttcacgat gccgccgatc ctgcgcgtct tcggcgccaa
     2881 cagcagcctc gcggtgatca tcgacggcaa ggaccggccc tacggcgtgc tcgacgtgca
     2941 cgcgaccgtg cccggccgct tcaccgccaa ggacgtgccc ttcgtccagg gcgccgccaa
     3001 cgtgctggcg gatgccttcg agcgccacgc cgccgaccag gcgctgcgcc accgcgtcct
     3061 ccacgactcg ctcaccggcc tgcccaaccg actctccttc gtcgacgcgc tcggcgaggc
     3121 gctgcgccgc gccgaggtct ccggctcccc cgtcgggatc ctcttcctcg acctcgacca
     3181 cttcaagctg atcaacgact ccctcggcca ccacgccggc gacgccctgc tgcgggcggt
     3241 ggcgccgcgc ctgcgcggcc acctccgtcc tggcgacatc gtcgcccgct tcggcggcga
     3301 cgagttcggg atcctgatcg accgcctggc cgacgagggc gaggcggtgg cgatcgccga
     3361 ccgcgtctcc gccgccttca cccagccctt cgcgatcgac ggggtcgagc acttcgtcag
     3421 ctccagcatc ggggttgccg tcgcccgcag ctccgagcgc caggtcaacg ccgagctgct
     3481 catccgcgat gccgacgccg ccatgtatcg cgcgaaggag agcggccgcg cccgctgcgt
     3541 gctcttcgac gccgagatgc gggccggggc gatgcgccgg ctcgaggtcg aacgcgagct
     3601 gcgcacggcg ctcgaccgcg acgagcttgc cctccactat cagccggtgg tcagcctggg
     3661 caccggcgag atcaccggcc tcgaggcgct ggtccgctgg cagcaccccg agcgcggcct
     3721 gctcgacccg gccgagttcg tctcgatcgc cgaggacagc ggcctgatcg agccgctcgg
     3781 gcgctgggtc caggagcgcg cctgccagca ggcggtcgaa tggcagaacg agcgccccga
     3841 cgcccgtccc ttcgacgtcg ccgtcaacct ctccgcccgc caggtcgccc accgcgacct
     3901 gcccgcgacc gtcgccgaga tcatcgcccg caccgggctc gacccggtcc acctgcggct
     3961 ggagatcacc gagagcgtgc tggtcgagga gtcggcctcg gcgatctcat cgctggaagc
     4021 gctgaacgag atcggcgtcc gcctcgtcct cgacgacttc ggcaccggct actcctcgct
     4081 cgcctacctc aaccgctacc ccttccacgc gctgaagatc gaccgctcct tcgtcgacgc
     4141 gctcgggatc gaacaggagc gcacggcgat cgtcgaggcg atcatcggca tggcccgggc
     4201 gctctcgctc gaggtgatcg ccgagggcgt cgagaacgag gtccagctat cggagctgcg
     4261 tcgcctcggc tgcgactacg cgcagggcca cctcttccac gcggcgatgc ccgacaccca
     4321 ggtcagccgc atgctcgagg aaggcgcgct cggctattcg gcggggatct cgcccggcaa
     4381 gtagtcgttg ctgagcagtt cggcggcgct cggcagcgac tcgatcagtt cgttgtcgcg
     4441 catccagccg gcgaactcgt cccaggcggt cgggtccatg tagccgtagg gctggccctc
     4501 ggtgcgggcg ccgagcagcg gcagcgtcgc ttccacctcg gcccgggtga gtttcggttc
     4561 gagcgcgtcg ttggcggcga ggatcgcctc ggtggcggcg gccggctgtg cgaccgcggc
     4621 gttggtgccg cgctccagcg cggccatgaa aaggcggaac ttctccggtt cggcttccac
     4681 ggtctcccgt ttggcgacca gcacgagctc gtcgtaggtc ggtacgccga gctggtcgac
     4741 cggggtgacg accggtttga gcccgcgttc gcgcaggtcg acgccctcga cgttgcggaa
     4801 gccgccgagc atcgcgtcgg cgctgccgcc gaccagcgcc ggcagcaggt tgaagccgac
     4861 gttgaccgct ttcacgtcag aggtggtcag cccggcgcgg gtgaggatcg tctccaggaa
     4921 cgcctcctgg taggggatgc cggcgtaggc gatcgttttc cctttcagcc cgggcacggc
     4981 tttgatcccc gatttctgca gccagatcat cgaggtcagc ggctcgttga ccagcgcccc
     5041 gatcgcgacc acgtcgagcc cccgctcgtg ggccaggatc acctccggct cgtaggagat
     5101 cgccaggtcg gtgcggcccg cggcgacctg cttgatcggc agcgaggggt cggtcggcgc
     5161 ttcgatcccg acctcgagcc cggcttcctc gaagtagccg agcttctcgg ccatgtagat
     5221 cccggcgtgg tcggggttgg ggtagaagtc gagcgccagc gagagcgact gcgagtcggc
     5281 gccgctggaa tcctcggact tctcgccgca ggcggcgagc gtgagggcga gcaggatggc
     5341 gagggcggtg acgatcgcca cgaggcgggg ggtcttcatt ccctgtgttc tatcgccaag
     5401 tgatgatgcg acgctctgcg agggcgacga gggcgtaaag agcgatcgcg atcgcggaga
     5461 gaacgacgag cgcagcgaag gtcagcgcga cctgaaagtt ggcgttggaa atgaggatca
     5521 ggcggccgag gccctcattg gcgccggccc actcggcgaa gacggcgccg atcggggcga
     5581 tcgccgcggc gatccgggcg ccgctgaaga acgagggcag cgccccacgc gactcgaccc
     5641 ggcggaaggt ttgcatgcgc gaggcgtaga gcgtgcgcat cagcttgatc gcggccgggt
     5701 cggccgagcg cagcccgtcg agggtggcga cggtcaccgg gaagaagcag atcaaagcga
     5761 tgatgatcag cttcggggtg atcccgaagc cgaaccagat cacgaagatc ggcgcgatca
     5821 cgatgaccgg gatcgtctgc gagacgatca ccagcgggtt ggcagcgagg cggaccgtgg
     5881 tcgagaagtg catcgccact gcgaacgcga ctccgacgac gaccgcgagc aggaacccga
     5941 ggacgatctc ctgcagcgtc acccagccgt tttcgagcag cagcgtgcgc tcctcccaga
     6001 gctggctggc gatttcggcg ggcgagggga cgaggaagtc ttcgaggttc atcacctcgg
     6061 cgaggaagcc ggtgctggcg gcgagctgcc agagcccgat caacgccgcc agcagcgcca
     6121 gcgggtagag ccagcgcttc accgcgagcc ctcgtgcagc gcccgcagcg cccgttcgcg
     6181 ggcggcgacg aaggcggggt cggtgacggc ggcgtcgcgg tcggcggcgc gcgggtcggg
     6241 cgcgtcgagc tcggcgacga tccgcgccgg gcgggcggag agcacggcga cgcggtcggc
     6301 gaggtagagc gcctcctcga cgtcgtgggt gacgaggacg acggtgcggg gatcggtgcg
     6361 cagcgcgctc gccagccact cctgcatctc ggcgcgggtg atcgcgtcga gcgccgcgaa
     6421 cggctcgtcg agcgccagca ccggcttgcc ggagacgagg gtgcgcagga aggcgacccg
     6481 ctggcgcatc ccaccggaga gctcgccggg cttcatcgcc tcgaagccgg cga
//
LOCUS       DASRIG010000278         5352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_916507, whole genome shotgun sequence.
ACCESSION   DASRIG010000278 DASRIG010000000
VERSION     DASRIG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916507"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFB52_07680"
     CDS             complement(<1..241)
                     /locus_tag="VFB52_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06251.1"
                     /translation="MAEREQVGSVASGDPIEGSAVYPVGSRVNERGHLEVAGCDVVEL
                     AAEHGTPAYLYAEDDIRSRAREYRQAFERRGAEFEV"
     gene            complement(288..1004)
                     /locus_tag="VFB52_07685"
     CDS             complement(288..1004)
                     /locus_tag="VFB52_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06252.1"
                     /translation="MVRQAHTYLVGAMSGATLIAIAIASFVLLVSAQVFTDWPIPGLS
                     GAKETAVHENEAVGDGNAGAATGAAAGGAATSAATAAADTNAGAGGNAGATGADQLRG
                     GATTDGEGTNEAVSGGGGGGDQGGSGTTPSSETPAAGGGGNSSPSTGGGSGGSSGGGS
                     SSGGGAETASTPSQTVTDTVNDTVTKVDETALGGALGDTGVTGVTEGVVDGVAGPESA
                     VGKVVDGVAGTVGGLLGSGK"
     gene            complement(1091..2041)
                     /locus_tag="VFB52_07690"
     CDS             complement(1091..2041)
                     /locus_tag="VFB52_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZO06253.1"
                     /translation="MHSHDHDHAGHSHGYGAHALEARRADSRKRLWVALAINVGLLVA
                     EVVGGLITGSLAVLADAGHVLSDAGAIALALFAARLAALPAAGRRTFGYQRSEVLAAL
                     VNGITLVVVSVVIAVAAIGRFSDPPEIEGWGVLALGIFGLAGNLAATLVLARGQREDI
                     NLEGALRHSAADALGSIGVVVAGAWVLLGGSPIVDPIVGLLIAVLVLASSWRLIKEPL
                     DVLMEAAPSNLDVDRVGAAICEEDGVRSVHDLHVWTVTAGFGAIAAHVVVAEGSDRDL
                     IRRRLELTLGEQFGIEHTTLQMEEEAGQGLLSVENAPEPR"
     gene            complement(2062..3333)
                     /locus_tag="VFB52_07695"
     CDS             complement(2062..3333)
                     /locus_tag="VFB52_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06254.1"
                     /translation="MNFELNEEQRAFKEKCRTFAREVIRPAARKHDEEESTPWEVVKE
                     ARKQGLQGIEYLQMTAADGDGQMGAIAAEEMHWGCAGTALAINGSGLAAAGIAASGTP
                     EQIGYWVPECFGTGDEIKLGAYAVTEPQAGSDVKSLKTTAKRDGDEWVLNGTKVFITN
                     GGIADVHVVVATIDPELGTRGQASFVIGKDTPGLSQGKKETKLGIRASHTAEVVLEDC
                     RIPVENLLGGMEKLEKKLERARSGQSTRSGADALATFEITRPIVGASALGIAQAAYEW
                     TLEYLDGQSEGGVPMLKIQRVQQTLADVATEIEAARLLVQRASWMGRNGVPMTGGQGS
                     MSKLKAGDVTMWATTTLMDLVGPDAWNTEYPLEKFFRDAKIYQLFEGTAEIQRMVISR
                     MQAREYAERLGFGLEVAAEAVKTEQKEPVPA"
     gene            3412..4704
                     /locus_tag="VFB52_07700"
     CDS             3412..4704
                     /locus_tag="VFB52_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06255.1"
                     /translation="MSADRPINTEEAPTDEQLVPKEEPKPAPSSAPSPEPEPAPAPPR
                     PPRKPKPLAPKLLALLVVLAVGFIVAAIVFGSGGGKDETSAEQATPAPSVEPSGDGEG
                     AATAESLGFPGFATNNTTRVGGADPAANAAAVALAAFPATTESQRPKAVTLIDEGDWA
                     GAIAAAVLMAPPVRAPVLFSSEGAVPQATEEALAALQPKNTHEVAGSGAAAAGEIAIL
                     RDRLFPKAPKHIVIAPADAPEFAMPAAAWAARSGDPVLFASGDDLPPATVSYLERHNQ
                     LPIYVLGPSAAISSEILREAEKIGGDARRVSGEDPVANAIAFARYDDGSFGWNINDPG
                     HGFVLVRDDAPLDAAAAAPLSGSGTWGPLLLTDSADTLPAELRDYFLDVKPGYTDNPT
                     RAFYNHVWVIGDQEAIDVNQQAEVNELAELAKIGSGGE"
     gene            4701..4946
                     /locus_tag="VFB52_07705"
     CDS             4701..4946
                     /locus_tag="VFB52_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06256.1"
                     /translation="MSEAENPQLLGRGEVTPEDIRALAGPSTPHFALQVRNRIRRLIE
                     PLPADHPARVEGERQIARLEDLSKHSGDPRGTLGISH"
     gene            4948..>5352
                     /locus_tag="VFB52_07710"
     CDS             4948..>5352
                     /locus_tag="VFB52_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO06257.1"
                     /translation="MKYLAPGVWRLKEFPAPTINVYLAEDVLIDAGRRWDRGRIFAEL
                     EGREISLLALTHAHPDHQGVAKDVCEARGVPLACHADDVEAMEGTRSMQEAAENNPIN
                     RVIRAIWQGPPYKVDRPLKEGDEVAGFRVVHAP"
BASE COUNT          774 a         2011 c         1799 g          768 t
ORIGIN      
        1 ggacctcgaa ctcggccccg cggcgctcga acgcctggcg gtattcgcgg gcgcgggagc
       61 ggatgtcgtc ctcggcgtag aggtaggccg gggtaccgtg ctcagcggcc agctcgacca
      121 cgtcgcagcc ggcaacctcg aggtggccgc gctcgttgac ccggctcccc accgggtaga
      181 ccgccgaacc ctcgatcgga tcaccactgg cgaccgaccc cacctgttct cgctccgcca
      241 tcggcggcat tctgacgatt cccagctcag ctaggaccca gcggtcccta tttaccgctg
      301 ccgagcaggc cgccgaccgt gccggcgact ccgtcgacga ctttgccgac cgccgattcg
      361 gggccggcga cgccgtcgac gacgccttcg gtgaccccgg taacgccggt gtcgccgagc
      421 gctccgccga gcgccgtttc gtcgactttg gtgaccgtgt cgttgacggt gtcggtgacc
      481 gtctgcgaag gcgtcgaggc ggtttcggca ccgccacccg aggaggagcc gccgccggag
      541 ctaccgccac tgccgccgcc ggtcgatggc gaggagttac ccccgccccc agccgccggc
      601 gtctcgctgg agggcgtggt gccggagcca ccctggtccc cgcccccacc gccgccgctg
      661 acagcttcgt tggtgccttc gccgtcggtg gtcgcaccgc cgcgcagctg gtccgccccg
      721 gttgcgccgg cgttgccacc cgcacccgca ttcgtgtccg ctgcggccgt cgcggcggac
      781 gtcgccgccc cgcccgcagc cgccccggtg gctgcaccgg cgttgccatc gccgaccgcc
      841 tcgttctcgt gcacagccgt ctccttggcg ccgctcaggc cgggaatcgg ccagtcggtg
      901 aagacctgcg cggagacgag caggacaaag cttgcgatgg cgatggcgat cagcgtcgcc
      961 cccgacatcg caccgaccag ataagtgtgc gcctgacgaa ccatctccga ttgctaaagg
     1021 taacccatcg aatcgccccg gtggccatat atccggctct ggaaagcgca aacctgcgga
     1081 acaggcaggc tcaacggggc tcaggcgcgt tctcgacgct gagcaggccc tgtccggcct
     1141 cctcctccat ctgcagcgtc gtgtgctcga tcccgaactg ctcgcccagg gtgagctcga
     1201 ggcggcggcg gatcaggtcg cggtcggagc cctcggcgac gaccacgtgg gcggcgatcg
     1261 ccccgaagcc cgcggtcacg gtccagacgt gcaggtcgtg gaccgagcgc accccgtcct
     1321 cctcgcagat cgcggccccg accctgtcga cgtcgaggtt ggagggcgcc gcctccatca
     1381 gcacgtcgag cggctccttg atcaggcgcc aggaggaggc gaggacgagc acggcgatca
     1441 gcaggcccac gatcgggtcg acgatcggtg agccgccgag caacacccac gcgcccgcga
     1501 ccacaacccc gatcgagccg agcgcgtcgg cggcggagtg gcgcagcgcc ccctccaggt
     1561 tgatgtcctc gcgctggccg cgggcgagga ccagggtcgc ggcgaggttg ccggcgagcc
     1621 cgaagattcc gagcgccagc acgccccagc cctcgatctc gggcgggtcg gagaaacggc
     1681 cgatcgcggc gaccgcgatc acgacgctga ccacgaccag ggtgatgccg ttgaccagcg
     1741 ccgccagcac ctcggagcgc tggtagccga aggtgcgccg gccggccgcc ggcagcgccg
     1801 ccagccgcgc cgcgaacagc gccagcgcga tcgcgccggc gtcggagagc acgtggccgg
     1861 cgtcggcgag caccgccagc gagccggtga tcagcccgcc gacgacctcg gcgaccagca
     1921 ggccgacgtt gatcgccagc gcgacccaga gccgcttccg gctgtccgcg cgccgcgcct
     1981 cgagcgcgtg cgcgccgtag ccgtggctgt gcccggcgtg atcgtggtcg tgcgagtgca
     2041 cgccccgagg ataggcccga gctaggccgg gaccggctcc ttctgctcgg tcttcacggc
     2101 ctcggcggca acctcgaggc cgaagccgag gcgctcggcg tactcgcgcg cctgcatccg
     2161 cgagatcacc atgcgctgaa tctccgcagt gccctcgaac agctggtaga tcttcgcgtc
     2221 gcggaagaac ttctccagcg ggtactcggt gttccaggcg tcggggccga ccaggtccat
     2281 cagcgtggtc gtcgcccaca tcgttacgtc gccggccttg agcttcgaca tcgagccctg
     2341 gccgccggtc atcgggacgc cgttgcggcc catccaggag gcgcgctgga ccagcaggcg
     2401 cgccgcctcg atctcggtcg cgacgtcggc cagcgtctgc tgcacgcgct ggattttcag
     2461 catcggcacg ccgccttcgg actggccgtc gaggtattcg agcgtccact cgtaggccgc
     2521 ctgggcgatc ccgagcgccg aggcgccgac gatcggccgg gtgatctcga aggtggcgag
     2581 agcgtccgcc ccggagcgcg tgctctgccc cgagcgcgcc cgctccagct tcttttccag
     2641 cttctccatg ccgcccagca ggttctcgac cgggatccgg cagtcctcga gcacgacctc
     2701 ggcggtgtgc gaggcgcgga tcccgagctt ggtctccttc ttgccctggg agaggcccgg
     2761 cgtgtccttg ccgatcacga acgaagcctg cccgcgggtg ccgagctccg gatcgatggt
     2821 cgcgacgacc acgtgcacgt cggcgatccc gccgttggtg atgaagacct tggtcccgtt
     2881 caggacccac tcgtcgccgt cgcgcttcgc ggtcgttttc aggctcttca cgtcggagcc
     2941 ggcctgcggc tcggtgaccg cgtaggcgcc gagtttgatt tcgtcgccgg tcccgaagca
     3001 ctccggcacc cagtagccga tctgctcggg cgtcccggag gcggcgatcc cggcggccgc
     3061 gaggccggag ccgttgatcg cgagcgcggt gccggcgcag ccccagtgca tctcttcggc
     3121 agcgatcgcg cccatctggc cgtcgccgtc ggcggcggtc atctgcaggt actcgatccc
     3181 ctgcaagccc tgtttgcgcg cctccttgac tacctcccag ggggtgctct cctcttcgtc
     3241 gtgcttgcgc gcggcggggc ggatcacctc gcgggcgaag gtccggcact tctctttaaa
     3301 cgcgcgctgc tcctcattca gttcgaaatt cattcctctg ccttttccgg ggcctgggcg
     3361 gttgattgac tggtcagtca gccagtccga ataagtgtag cgttcgggcc ggtgagcgcc
     3421 gaccgcccaa tcaacaccga ggaggcgccg acggacgagc agctggtgcc gaaggaggag
     3481 ccgaaaccgg caccctcgtc ggccccatcg cccgagcccg agccggcccc ggctccaccc
     3541 cgcccgcccc gcaagccgaa gccgctggcg ccgaagctgc tggccctgct ggtcgtgctc
     3601 gcggtcggct tcatcgtcgc cgcgatcgtc ttcggcagcg gcggcggcaa ggacgagacc
     3661 agtgccgagc aggcaacgcc cgcgccgagc gttgagccca gtggggacgg cgagggcgct
     3721 gcgacggccg aatcgctcgg cttcccgggc ttcgcgacca acaacacgac ccgggtcggc
     3781 ggcgccgacc cggcggcgaa cgcggccgcc gtcgccctcg cggccttccc cgcgaccacc
     3841 gagtcgcagc ggccgaaggc ggtcacgctg atcgacgaag gcgactgggc cggcgcgatc
     3901 gccgcggcgg tgctgatggc gccgccggtc cgcgctcccg tgctcttctc cagcgagggc
     3961 gccgtcccgc aagcgaccga agaagccctt gccgccctgc agccgaagaa cacccacgag
     4021 gtcgccggct ccggcgccgc cgcggccggc gaaatcgcga tcctccgcga ccgcctcttt
     4081 cccaaggccc cgaagcacat cgtgatcgcc cccgctgacg cgcccgagtt cgcgatgccg
     4141 gccgccgcct gggccgcccg ctccggcgac cccgtgctct tcgccagcgg cgacgacctg
     4201 ccgccggcaa ccgtctccta cctcgaacgc cacaaccagc tgccgatcta cgtgctcggg
     4261 ccgtccgcgg cgatctcctc ggagatcctc cgtgaagccg agaaaatcgg cggcgacgcc
     4321 cgccgcgtct ccggggagga cccggtcgcc aacgcgatcg ccttcgcccg ctacgacgac
     4381 ggcagcttcg gctggaacat caacgacccc ggccacggct tcgtcctcgt ccgcgacgac
     4441 gcgccgctcg acgccgccgc cgcggcgccg ctctcgggct cggggacctg ggggccgttg
     4501 ctgctcaccg acagcgccga tacgctcccg gccgagctac gcgactactt tctcgatgtg
     4561 aagcccggct ataccgacaa cccgacccgc gccttctaca accacgtgtg ggtgatcggc
     4621 gaccaggagg cgatcgacgt gaatcagcag gcggaagtga acgagctggc cgaactggcc
     4681 aagatcggca gcggcggcga atgagcgagg ccgagaaccc ccagctgctg ggccgcggcg
     4741 aggtgacgcc ggaggacatc cgcgcgctcg ccggcccctc caccccccac ttcgcgctgc
     4801 aggtgcgcaa ccggatccgc cgtctgatcg agccgctgcc cgccgaccac ccggcgcggg
     4861 tcgaaggcga gcgccagatc gcccgcctcg aagacctctc gaagcacagc ggcgacccgc
     4921 gcggcaccct cggcatcagc cactgagatg aagtacctgg ccccaggcgt ctggcggctg
     4981 aaggagttcc cggcgccgac gatcaacgtc tacctggccg aggacgtgct gatcgacgcc
     5041 ggccggcgct gggaccgcgg gcgcatcttc gccgagctcg agggccgcga gatctcgttg
     5101 ctggcgctga cccacgccca cccggaccac cagggcgtcg ccaaggacgt ctgcgaagcg
     5161 cgcggcgtgc cgctcgcctg ccatgccgac gacgtcgaag cgatggaagg gacgcgctcg
     5221 atgcaggagg cggcggagaa caacccgatc aatcgcgtga ttcgggcgat ctggcaggga
     5281 ccgccctaca aagtcgatcg ccccctcaaa gagggtgacg aggtcgccgg cttccgggtc
     5341 gtccacgcgc cc
//
LOCUS       DASRIG010000279         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1451249, whole genome shotgun sequence.
ACCESSION   DASRIG010000279 DASRIG010000000
VERSION     DASRIG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451249"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>2035
                     /locus_tag="VFB52_07715"
     CDS             45..>2035
                     /locus_tag="VFB52_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HZO06258.1"
                     /translation="MSSLANPPVRRRRLRALLALAGAALASTALATSAAAAPATGDVV
                     LTLKKGAKSSLLRDGVKVSPRSGKGATQKVTLKVTDADAASKLVKTGATLTFRANGKS
                     VKVSGVELKAGAQSTAISGVQGGKRKVFFRLQGESLAVGNALSAKGPLALTGAGAKAL
                     GTALGIDGIKSGKIGSAGFSAVITTTPAPEPPKKEEKEPPKNPYPYEAQCPVAAVEGN
                     PGFGEAPGKIESTLPLPVFNPDFSREVTGGGSDWGFKDSFRSYVLLVPPSGSLQALEG
                     ATANPAGPTMGIPGSFWSFPTSEGDYEPGVEGDLSDDKLVIDGEGTILFCKSGHGFNV
                     VLKNPTVVIDGANSRIVADVGANFNGTWYPLQRADIATLDLDAVEPELTDGNTLAIWE
                     DLPVELTADGEKALGGIYEAGEELDPITVEASLGPEPPEPYPYGTECPVGAVEGGMGD
                     DPGVVDGIAPAPAFNAGTSQKLTGTANNWGFKSGFRGYVLGAPPAGTLQTLDGATASA
                     AGMGMAVPTAYFGFPTGGGTYEPGTQPDHSDDKLVSNSTGTVLFCKPGHGFNVVLKNP
                     TVTIDGDKSRVTADVGVNLSGKWFPFQRVDIATLDLDGVEPEFTESGNKVAWKEVPAT
                     LTEDGETALGGIYPAGEALDKITVETALERPLLTECTIAS"
BASE COUNT          379 a          752 c          653 g          251 t
ORIGIN      
        1 agggcgccct tcctcacttc gaccgctagg agaccacgcc tcagatgagc tcgcttgcca
       61 atccccccgt ccgtaggcgg cgcctgcgcg ccctgctcgc gctggccggc gccgcgctcg
      121 cctcgaccgc gctcgccacc agcgccgccg ccgcccccgc caccggcgac gtcgtcctga
      181 ccctgaagaa gggcgcgaag agctcgctcc tgcgcgacgg cgtcaaggtc agcccgcgca
      241 gcggcaaagg cgcgacccag aaggtcacgc taaaagtcac cgacgccgac gccgccagca
      301 agctggtcaa gaccggcgcg acgctgacct tccgcgccaa cggcaagagc gtgaaagtgt
      361 ccggcgtcga gctcaaggcg ggcgcgcaga gcaccgcgat cagcggcgtg cagggcggca
      421 agcgcaaggt cttcttccgc ctccagggcg agtccctggc ggtcggcaac gcgctcagcg
      481 ccaaaggccc gctggccctg accggcgccg gcgcgaaggc gctgggtacg gcgctcggca
      541 tcgacgggat caagagcggc aagatcggct cggccggctt ctcggccgtg atcacgacca
      601 cgccggctcc ggaaccgccg aaaaaagaag aaaaagaacc gccgaaaaac ccgtacccct
      661 acgaggccca gtgcccggtg gccgcggtcg aaggcaaccc cggcttcggt gaagcccccg
      721 gcaaaatcga aagcaccctg ccgctgccgg tgttcaatcc ggacttcagc cgggaagtga
      781 ccggcggtgg gtccgactgg ggcttcaagg acagcttccg cagctacgtc ctcctggtcc
      841 cgccgagcgg ctccctgcag gcactcgaag gcgcgaccgc aaacccggcc ggcccgacga
      901 tgggcattcc cggctccttc tggtccttcc cgaccagcga gggcgactac gaacccgggg
      961 tcgaaggcga cctctccgac gacaagctcg tgatcgacgg cgaaggcacc atcctcttct
     1021 gcaagtccgg ccacggcttc aacgtcgtgc tgaagaaccc gaccgtcgtg atcgacggtg
     1081 ccaactcgcg gatcgtcgcc gacgtcggcg ccaacttcaa cgggacctgg tacccgctgc
     1141 agcgcgccga catcgccacg ctcgacctcg acgcggtcga gccggaactc accgacggca
     1201 acaccctggc gatctgggag gacctccctg tcgagctcac ggccgacggc gagaaagccc
     1261 tcggcggcat ctacgaagcg ggcgaagagc tcgacccgat cacggtcgaa gcgtcgctcg
     1321 gccccgaacc gccggaaccg tacccgtacg ggaccgagtg cccggtgggc gcggtcgaag
     1381 gcggcatggg cgatgacccc ggcgtcgtcg acggcatcgc cccggcgccg gccttcaacg
     1441 ccgggaccag ccagaaactc accggcaccg cgaacaactg gggcttcaag tccgggttcc
     1501 gcggctacgt gctcggcgcg ccccctgcgg gcacgctgca gacgctcgac ggggcgaccg
     1561 cgagcgcggc cggcatgggc atggccgttc cgaccgccta cttcggcttc cccaccggcg
     1621 gcggcaccta cgagccagga acccagcccg accactccga cgacaagctc gtctcgaaca
     1681 gcaccggcac cgtgctcttc tgcaagccgg gccacggctt caacgtcgtg ctgaagaacc
     1741 cgacggtgac gatcgacggc gacaaatcgc gggtcaccgc ggacgtcggt gtcaacctca
     1801 gcggcaaatg gttcccgttc cagcgggtcg acatcgccac gctcgacctc gacggggtcg
     1861 agccggaatt caccgaaagc ggcaacaaag tcgcctggaa agaagttccg gcgaccctga
     1921 ccgaagacgg tgagaccgcg ctcggcggga tctacccggc gggcgaagcg ctcgacaaaa
     1981 tcacggtcga aaccgcgctc gaacggccgc tgctgaccga gtgcacgatc gcctc
//
LOCUS       DASRIG010000280         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_919194, whole genome shotgun sequence.
ACCESSION   DASRIG010000280 DASRIG010000000
VERSION     DASRIG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919194"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFB52_07720"
     CDS             complement(<1..272)
                     /locus_tag="VFB52_07720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HZO06259.1"
                     /translation="MALAAAPAQAIVAGTPAKRADYPFFAVIGSGCGGALVAPDRVLT
                     AAHCTEALNSRDDVRVGPRNARRTVRRLAINPLHVRELAKMEREFPP"
     gene            complement(302..1456)
                     /locus_tag="VFB52_07725"
     CDS             complement(302..1456)
                     /locus_tag="VFB52_07725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZO06260.1"
                     /translation="MLRVSALVTLALLLLLAPATAQAAPEEQWTPLVASVVAEPEPVE
                     AADGRVHLAYELLLINRSFLPPAKVTVKGVQVLAGGKVVASLSAKKLREVMVRFSDPT
                     KPGTTLDKGEAGFVLMDVTFERGEKVPRRLTHRLRIATKPLNTTAAHNYVAAPTPVSK
                     RTALVVAPPLRGDGWIVGNGCCEALTSHRGALLPVNGSLVNGERYAIDFIQIQPNGML
                     ATGPLTELSSYPFFGDEVISATAGKVVRVVDRYPETPPGALPPSTAAGAGGNYVSVEV
                     GGGRYAFYAHLQPGSIKVKVGDRVQPGQLLALLGSSGNSNAPHLHFQLMDGPTPVASS
                     GIPYRFSRFSVAGTLTNFGGLFDDQPAQIAPKLRGVHRKQLPLALQVIGF"
     gene            complement(1505..2236)
                     /locus_tag="VFB52_07730"
     CDS             complement(1505..2236)
                     /locus_tag="VFB52_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZO06261.1"
                     /translation="MRVEMFNVGWFTSAAGIWRQGDEMERQVRFPVPAYLIETESERI
                     LVDTGLAPAAIPDPVAHYGFEALSAFGFEQERSVADQVDLDSLTKVVMTHLHFDHAGG
                     LSLLPPSLPVYIQQREWAAAHDLTASRKNFLLAPDYATIADQVIVVDGDLDLLGDGSV
                     ELLSTPGHTAGHQSVRVGEELIIGGDVSHYASGLDDHRFPVFADDFEAQKASAERLRG
                     LRDAGATVRPGHDPELLVPGPIVQA"
     gene            2312..>2680
                     /locus_tag="VFB52_07735"
     CDS             2312..>2680
                     /locus_tag="VFB52_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06262.1"
                     /translation="MRTRLLVSLALSALAATALALPVATGAITIPKGVKSAKVTRYPV
                     TVDAVGYLDYTWTWDSTQPCVPGYAKTIEESLTFELGKPRAGSAQVVNGQAIIFPVKG
                     GDATVQTELSGWRTTNYCPPT"
BASE COUNT          392 a          903 c          979 g          406 t
ORIGIN      
        1 ggcgggaact cacgttccat cttcgccagc tcgcgcacgt gcagcgggtt gatcgcgagc
       61 cgccgcacgg tgcggcgcgc gttgcgtggc ccgacccgga cgtcgtcgcg ggagttcagc
      121 gcttccgtgc agtgggcggc ggtgagcacg cggtcggggg caacgagggc cccgccgcag
      181 ccgctcccga tcaccgcgaa gaaggggtag tcggcgcgtt tggccggggt gccggcgacg
      241 atcgcctggg ccggtgcggc cgccaacgcc aacaggcagg cggcgaggag gagcgcccgc
      301 ttcagaaccc gatcacctgc agcgcgagcg gcagctgttt gcggtggacg ccccgcagtt
      361 ttggcgcgat ctgcgccggc tggtcgtcga acaggccgcc gaagttggtc agcgtgcccg
      421 ccaccgagaa gcggctgaag cggtagggga tcccgctcga ggcaaccggg gtcggcccgt
      481 ccatcagctg aaagtgcagg tggggggcgt tcgagttgcc gctcgagccg agcagggcca
      541 gcagctgccc cggctgcaca cggtcgccga ccttaacttt gatgctgccc ggctgcaggt
      601 gggcgtagaa cgcgtagcgg ccgccgccga cttcgaccga gacgtagttg ccgcccgccc
      661 ccgccgcggt gctcggcggc agggcgccgg gtggcgtctc cgggtacctg tcgacgacgc
      721 ggacgacctt gccggcggtc gccgagatca cttcgtcgcc gaagaagggg tagctgctca
      781 gctcggtcag cggcccggtg gcaagcatcc cgttcggttg gatctggatg aagtcgatcg
      841 cgtagcgctc gccgttgacg aggctgccgt tgaccggcag cagcgcgccg cggtgcgagg
      901 tgagcgcttc gcagcagccg ttgccgacga tccagccgtc gccgcgcagc ggcggcgcaa
      961 ccaccagggc ggtgcgcttg gagaccggcg tcggcgccgc gacgtagttg tgcgccgcgg
     1021 tcgtgttcag cggcttggtt gcgatccgca ggcggtgggt gaggcggcgt gggaccttct
     1081 cgccgcgctc gaaggtcacg tccatcagca cgaagccggc ttcgcctttg tccagcgtgg
     1141 ttcccggttt ggtcgggtcg ctgaagcgga ccatcacttc gcgcagcttc ttggcggaga
     1201 gcgaggcgac gaccttgccc ccggccagca cctgcacgcc cttcaccgtc accttcgccg
     1261 ggggcaggaa ggagcggttg atcagcagca gttcgtaggc gaggtggacg cggccgtcgg
     1321 cggcctcgac cggttcgggt tcggcgacga cgctggcgac cagcggcgtc cactgctcct
     1381 ccggcgccgc ctgggcggtg gcgggcgcga gcaggaggag cagcgcgagc gtgacgaggg
     1441 ccgagactcg aagcatcgtt ggccagattc tcggccgaac gaatcgaatc ctttatccgg
     1501 tcatctaggc ctggacgatt ggtccaggca cgagcaactc ggggtcgtgc cccggccgca
     1561 ccgtggcgcc ggcgtcgcgc agcccgcgca gccgttcggc cgaggccttc tgcgcctcga
     1621 agtcgtcggc gaagaccggg aagcggtggt cgtcgagccc ggacgcgtag tggctgacgt
     1681 cgccgccgat gatcagctct tcgccgaccc gcaccgactg gtgcccggcc gtgtggcccg
     1741 gggtggagag cagctcgacc gagccgtcgc cgagcaggtc gaggtcaccg tcgacgacga
     1801 tcacctggtc ggcgatcgtc gcgtagtcgg gggcgagcag gaagttcttc cgcgaggcgg
     1861 tgaggtcgtg cgcggccgcc cactcgcgct gctggatgta gaccgggagc gagggtggca
     1921 gcagcgagag cccgccggcg tggtcgaagt gcaggtgggt catcaccacc ttggtcaggc
     1981 tgtcgaggtc gacctggtcg gcgacgctgc gctcctgctc gaagccgaag gccgagagcg
     2041 cctcgaaccc gtagtgggcg accggatcgg ggatcgcggc cggggcgagg ccggtgtcga
     2101 cgaggatccg ctcgctctcg gtctcgatca ggtaggcggg caccggaaag cgcacctgcc
     2161 gctccatctc gtcgccctgt cgccagatcc cggcggcgga cgtgaaccag cccacgttga
     2221 acatctcgac gcgcatcccg cacccttcct atcaggcaag acccgtgctt tccctgatgc
     2281 gcaggtattc ctgcggagcg atgctggcgg tatgcgcacc cgtctgctcg tctccctggc
     2341 cctgtccgcc ctcgccgcca ccgcgctcgc gctccccgtc gcgaccggcg cgatcacgat
     2401 cccgaaaggg gtgaaatcgg cgaaagtgac ccgctatccg gtcacggtcg acgcggtcgg
     2461 ctacctcgac tacacctgga cctgggacag cacccagccc tgcgtccccg ggtacgcgaa
     2521 gacgatcgag gaaagcctca ccttcgagct cggcaagccg cgggcggggt ccgcccaggt
     2581 cgtcaacggc caggcgatca tcttcccggt gaaaggtggc gatgccaccg tgcagaccga
     2641 gctgagcggg tggcgcacga ccaactactg cccgccgacc
//
LOCUS       DASRIG010000281         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_668835, whole genome shotgun sequence.
ACCESSION   DASRIG010000281 DASRIG010000000
VERSION     DASRIG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668835"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFB52_07740"
     CDS             <1..204
                     /locus_tag="VFB52_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO06263.1"
                     /translation="AELRKRRIAANLTMRELAYHLDWSPSTVSRVESGYIGLPTTEVL
                     PPPGSSRAIRFSGSTANTSVKAG"
     gene            complement(396..1160)
                     /gene="istB"
                     /locus_tag="VFB52_07745"
     CDS             complement(396..1160)
                     /gene="istB"
                     /locus_tag="VFB52_07745"
                     /inference="COORDINATES: protein motif:HMM:NF005098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZO06264.1"
                     /translation="MSKTSELTHLFRTLKAPAAARAAPKLADRAREEEWSHERFLEAV
                     LSAEVASRESHGGENRIKAARFPARKTLEEFDFTFQRSVRKTVIEHLGQLDFCHAKEN
                     VVMLGPPGTGKTHLAIALGIRACLAGQRVQFATATEWVAKLGEAKRQGGLEAELRRLS
                     FVPLIVVDEVGYIPFDPEAANLMFSLVSARYERASMIVTSNKPFSAWGEIFGDEVTAT
                     AMIDRLVHHAEILSLKGDSYRLRDKDLGGPPPAERA"
     gene            complement(1157..2359)
                     /gene="istA"
                     /locus_tag="VFB52_07750"
     CDS             complement(1157..2359)
                     /gene="istA"
                     /locus_tag="VFB52_07750"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZO06265.1"
                     /translation="MEQWAEIRRMAKVERRSQREIHRLTGIHRDTIRRALAADEPPSY
                     GPRPKRPSKLGPFVGEIERLLDQEPTLSGVRIREEIEELGYCGGKTILDDLLRELRPR
                     YLPPPRTHQRTVYRPGELAQFDLTEPRREIPVGWDQLRKGYLVTCKLPYSRAFAGALV
                     FSKEFPDIAWGMSRCLERLGALPAKLVWDREGAIAPRGQPTEPFLAFCGQLALGWIVL
                     DAGDCQAKGALERDHRFVHGNFEAGRCFANPADFQDQLDRWCDRINERKHRSTRAIVC
                     ERLASEHERMRPLPEAMPSTDRRWVLRVPSQPYLRFDRNDYSLDPRLVGRRVEVRASQ
                     AEVIAVALDSGELAARHRRVFAGGLTFTDPAHQAALEELRGERKRRRREPEVEVRPLA
                     RYDALIPA"
BASE COUNT          390 a          891 c          942 g          410 t
ORIGIN      
        1 gcggaactcc gaaagcggcg gatcgccgcg aacttgacca tgcgcgaact cgcgtaccat
       61 ctcgactggt caccgtccac ggtctcccgg gtcgagtctg gctacatcgg gctgcccacc
      121 acggaagtct tgccaccgcc aggctcttcc cgcgcgatcc ggttctcggg ctcaaccgcg
      181 aacacgtctg tcaaggccgg ttgaaaactg gtcccccaga ttgggtaggg cgcccgcgga
      241 gcgaagcccg gagggcgagc ggagcgggcg ccggcgagcc gactgggacc cgccggccga
      301 aaattgaccc ccgaccccga tctgccccgg accggggccc tgagagccga tctgcggccc
      361 gcgacggcgc cggcgcgtgt gatccgtcgt ccgcttcagg cgcgctcggc cggcggtggg
      421 ccgcccaggt ccttgtcgcg aagccggtag ctgtcgcctt tgagcgagag gatctcggcg
      481 tggtggacga gccggtcgat catcgccgtc gcggtcacct catcgccgaa gatctcgccc
      541 caggccgaga acggcttgtt cgaggtgacg atcatcgagg cgcgctcgta gcgggccgag
      601 accaggctga acatcaggtt cgccgcctcg ggatcgaacg gtatgtagcc gacctcgtcg
      661 acgacgatca gcgggacgaa gctcaagcga cggagctcgg cctcgaggcc tccctggcgc
      721 ttggcctcac cgagctttgc cacccactcg gtcgcggtcg cgaactggac ccgctggccg
      781 gcgaggcagg cgcggatccc gagcgcgatc gccagatgcg tcttgccggt ccccggcggg
      841 cccagcatga ccacgttctc cttcgcatgg cagaagtcga gctggccgag gtgctcgatc
      901 acggtcttgc gcaccgagcg ctggaaggtg aagtcgaact cctccagcgt ctttcgggcc
      961 gggaagcgag ccgccttgat ccggttctcg ccgccgtggg actcccttga tgcgacctcg
     1021 gccgagagca cggcttccaa gaagcgctcg tgcgaccact cctcctcgcg cgctctgtcg
     1081 gcgagcttcg gagcggccct ggccgccgcc ggcgccttca gggtccgaaa caggtgggtg
     1141 agctcggagg tcttgctcat gccgggatca gggcgtcgta gcgggccagg ggccggacct
     1201 cgacctcggg ctctcgccgg cgtcgcttgc gctcaccgcg cagctcctcg agcgcggcct
     1261 ggtgggcggg atcggtgaag gtgagcccgc cggcgaagac ccggcggtgg cgagcggcga
     1321 gctcgccgct gtcgagggcg acggcgatga cctcggcttg ggaggcgcgg acctcgaccc
     1381 tccggccgac caagcgcgga tcgagcgagt agtcgttgcg gtcgaagcgc aggtagggct
     1441 gggacgggac ccgcaggacc cagcgccggt cggttgaggg catcgcctcg ggcaggggcc
     1501 gcattcgctc gtgctctgag gcgaggcgct cgcagacgat cgcccgcgtg ctgcggtgct
     1561 tgcgctcgtt gatccgatcg caccagcggt cgagctggtc ttggaagtcg gccgggttgg
     1621 cgaagcaccg gccggcctcg aagttgccgt gaacgaagcg gtggtcgcgc tcgagggccc
     1681 ccttggcctg gcagtcgccg gcgtcgagga cgatccagcc cagcgcgagc tggccgcaga
     1741 aggcgaggaa cggctcggtc ggctgaccgc gcggcgcgat cgcaccctcg cggtcccaaa
     1801 cgagctttgc cggcagcgca ccgagcctct caaggcagcg gctcatgccc caggcaatgt
     1861 cgggaaactc cttcgagaag accagggcgc cggcaaaggc gcgcgagtag ggcagcttgc
     1921 aggtgacgag atagcccttg cgcagctgat cccagccgac cgggatctcg cgccggggct
     1981 cggtgaggtc gaactgggcc agctcgccgg gtcggtagac ggtgcgctgg tgggtgcgcg
     2041 gcggtggcag gtagcggggg cggagctcgc gcaagagatc gtcgaggatc gtcttgccgc
     2101 cgcagtagcc gagctcttcg atctcctcgc ggatgcggac gcccgagagc gtcggctcct
     2161 ggtcgagcag tcgctcgatc tcaccaacga agggaccgag cttcgagggg cgcttcggcc
     2221 gcggcccgta gctcggcggc tcgtctgcgg cgagcgcgcg acggatcgtg tcgcggtgga
     2281 tcccggtcag gcggtgaatc tcccgctgcg atcgccgctc gaccttcgcc atccgcctga
     2341 tctccgccca ctgctccacg tcaagcaact gccttctccc ggtctcgacc tcttggtcga
     2401 gccagggtca gagcccggcc ggatggctcc ggccctgcgc cgagtggacc agttttagac
     2461 cggcgcaggt ggcccagtat tcaggcggcg cctacacgag gttgaccagc tgaaaggcgc
     2521 cgtcgatcgg cggccgggcc tcaacggggg agctacacca gtttcgctga gcgcatgctc
     2581 ctcaggattt ctcgatgagc gatcacccgc ggcagcggcc tcccttcttg gga
//
LOCUS       DASRIG010000282         6214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2759322, whole genome shotgun sequence.
ACCESSION   DASRIG010000282 DASRIG010000000
VERSION     DASRIG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759322"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFB52_07755"
     CDS             <1..1139
                     /locus_tag="VFB52_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZO06266.1"
                     /translation="GSETLPSWATWAEPHELGPYTVGMEEEVMLLDAHSWALAHRIDS
                     VLPRLPHGRTDSFTAETHGSALEIQTGVHARIGDAIEELAALRGELDETLRPLSMRAA
                     SAGTHPFAVWQEIVVSAGERYQFVYGSMRELARREPTFGLHVHVGVPDAEAAIFAANR
                     LRTHIPLLLALSVNSPFWQGRDTGLASARTPLFQAFPRVGIPRDFANYAEYVESVDVL
                     IRCDAVPEPTFLWWDVRPQPRFGTVEVRIMDAQTKLGESAAIAALVQSIVRAEVEGEE
                     HMERSMPQEVLSENRFLAARDGMEAEMIEPELGRRVPARELLADLVAVCRPHAQALGC
                     EAELDGVLKLGDRTGAERQIEQARALGSLPRLVGLLAADFLTRS"
     gene            complement(1153..2724)
                     /locus_tag="VFB52_07760"
     CDS             complement(1153..2724)
                     /locus_tag="VFB52_07760"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZO06267.1"
                     /translation="MKRISLVATVATLALLATATTGFAAREVYVPDYEADAVFVLDAT
                     TGAQIGAPIATGVDSAPYTLAISPDGNFVYTTNEDNGTVGVIDTRTKAVVGSITVGKS
                     PFGIAITPDGSRAFVSNPGDKTVSVIDLATRQTIGAPIPVGENAFAVAISPDGSRVFV
                     PNSSNDNVTVIDAKTLQVIGLPIPVGDNPYGLAVTPDGARVLVANNDDASLTAIDAKT
                     LQAVGAPIPVGNDPSGVTVSANGRTAYVGNFGSDTVSVVDLGTNQVAATIPGVPNAEF
                     VALTPTGQAGFVSENVGVAGLNLATNQLTGAHLKAEGAGQLAIVPDQSPTAAFQLQGR
                     ARPGVPAAFNASGSSDSDGTVGLYTWAFPTGASGSAGPKVRHSFPKPGKYSVTLTVTD
                     NEGCSVATVFTGQTAYCNGNPAATITKTVTVAYPGLKAKCPKRAKFGCAVAIQAVKWQ
                     GKGKKRRLVAQSAMAKATLKAGGTKILSVKPKKSARTRLAKAKRILVAQAVVERKSKS
                     EGVLRVRVAKLKVVR"
     gene            complement(2779..3231)
                     /locus_tag="VFB52_07765"
     CDS             complement(2779..3231)
                     /locus_tag="VFB52_07765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZO06268.1"
                     /translation="MNEPRGIDFSQISPEHIRGFIGGSFPGDLGIEPLEIGDDRTVAR
                     IVVDRRHLHPGGYVHGGVWTALGDTAAAWATFRAMPPYHDFTTIELKLNVFGSGLDGD
                     VLTAVAEPLHSGRNTVVLDVKVERERAGETKLAANLVVTQFVIPPRAG"
     gene            complement(3242..4399)
                     /locus_tag="VFB52_07770"
     CDS             complement(3242..4399)
                     /locus_tag="VFB52_07770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZO06269.1"
                     /translation="MPEAVIVDTLRTPIGRAFKGSLAQLRPDEMGAYVVDQLLERNPG
                     VDPAEIEDLFCGVGMPQGLQAFNVARIIALLSEKLPETVNGVTVSRYCASSLDSIRHA
                     ANAVKAGEGDVYIAAGVEWVSRYNERTEAAGVDDRNPNLIGENGQPNAYIDMGITAVN
                     VANKYEVSRADMDKYAQRSQELAVKSQEDGFFDREIVPVTLPDGTEVAKDDGPRASST
                     LEKLSELPEAFGGGGVTAGNSCPLNDGAAAVLIMSDTKAKELGLSPRARIVTAATHGN
                     EPELMGVAPIGAIKKVLDRAGMTIDDVGAVELNEAFAAQVIPIMDECDIPLEKMNTHG
                     GAIALGHPFGMTGARIMGTLLNVMETDDHQVGLETMCVAGGQGEAMIVERI"
     gene            4591..4740
                     /locus_tag="VFB52_07775"
     CDS             4591..4740
                     /locus_tag="VFB52_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HZO06270.1"
                     /translation="MPEGYCVKERKKVEIKDAKQVTMKNGRPAIQGFCPDCGTKIFKI
                     GKLEG"
     gene            4824..5330
                     /locus_tag="VFB52_07780"
     CDS             4824..5330
                     /locus_tag="VFB52_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO06271.1"
                     /translation="MVELSSERDVREAYAAHSGELYGFALRSLGDAGLAEEAVQETFV
                     RAWRAGERFDPEIASLRTWLFAILRNVVIDFGRARAARPKLTSDAGVEPSVEPLEQAL
                     LSWQVEEAMRRIGEDHRRVLVETHYRGRPYAEVAEELGVPEGTVKSRVYYGLRALRVV
                     LEEMGYEG"
     gene            5320..6033
                     /locus_tag="VFB52_07785"
     CDS             5320..6033
                     /locus_tag="VFB52_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZO06272.1"
                     /translation="MRAEEHRELRLSLGAYALGDLSPGERAAVEGHLRECAECRAEAE
                     ALVSVGRLLPLADPARFSEPAPRPPAELGERVAATIGAERRAERSQRRRRRGFGFALA
                     GATAAAALVLALVVFNGDGGGEDPATHVEFSSLPGKVEISAKLVPHAYGTEIHMYVKG
                     ARSGTLCQVFLRDQQGRRFSAGSFRYRWGDDSNAVLSSALDLSRTKSIAVNAGGHTYV
                     APVGQGPSAAIWNPTEEDA"
     gene            6033..>6214
                     /locus_tag="VFB52_07790"
     CDS             6033..>6214
                     /locus_tag="VFB52_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06273.1"
                     /translation="MKTSLKTNRVALLLAALALIAALAIAGCGGSDDGPEARGYGGGG
                     GGGADTTGGEITAAPNP"
BASE COUNT          856 a         2119 c         2238 g         1001 t
ORIGIN      
        1 tgggatcgga gacgctgccg tcctgggcaa cttgggcgga gccgcacgag ctcggtccct
       61 acaccgtcgg gatggaggag gaggtgatgc tgctggacgc ccacagctgg gcgctggcgc
      121 accgcatcga cagcgtcctg ccgcgtctcc cccacggccg caccgacagc ttcaccgccg
      181 agacccacgg ctcggcgctg gagatccaga ccggcgtcca cgcccggatc ggcgacgcga
      241 tcgaggagct ggcggcgctg cggggggagc tcgacgaaac cctgcggccg ctctcgatgc
      301 gagccgccag cgccggcacc caccccttcg cggtctggca ggagatcgtc gtctcggccg
      361 gcgagcgcta ccagttcgtc tacggctcga tgcgcgagct ggcccggcgc gagccgacct
      421 tcggcctcca cgtccacgtc ggcgtgcccg acgccgaggc ggcgatcttc gccgccaacc
      481 ggctgcgcac ccacatcccg ctgctgctcg cgctctcggt caactcgccc ttctggcagg
      541 ggcgcgacac cggcctcgcc tccgcccgca ccccgctctt ccaggccttc ccccgggtcg
      601 gcattccgcg cgacttcgcc aactacgccg agtacgtcga gtcggtcgac gtgctgatcc
      661 gctgcgacgc ggtgccggag ccgaccttcc tctggtggga cgtgcgcccg cagccgcgct
      721 tcggcacggt cgaggtgcgg atcatggacg cgcagacgaa gctcggcgag tcagcggcga
      781 tcgcggcgct ggtccagtcg atcgtccgcg ccgaggtcga gggcgaggag cacatggaac
      841 gctcgatgcc ccaggaggtg ctctccgaga accgcttcct ggccgcccgc gacggcatgg
      901 aggcggagat gatcgagccc gagctgggcc gccgcgtgcc cgcccgcgaa ctgctcgccg
      961 acctggtcgc ggtctgccgc ccgcacgccc aggcgctcgg ctgcgaggcc gagctcgacg
     1021 gggtcctcaa gctcggcgac cgcaccggcg ccgaacgcca gatcgagcag gcccgcgcgc
     1081 tcggttcact cccccgcctg gtcggcctgc tcgcggccga cttcctgacg cgcagctagc
     1141 ccggcacgcc gctcagcgca cgaccttgag cttcgcgacg cggacccgca acactccttc
     1201 ggatttcgac ttcctctcga ccaccgcctg ggccacgagg atccgctttg ccttcgccag
     1261 tctcgtgcgg gcgctcttct tcggcttcac ggagaggatc ttcgtgccgc cggccttcag
     1321 cgtcgctttc gccatcgcgc tttgcgcgac gagcctgcgt ttcttgccct tgccctgcca
     1381 cttgacggcc tggatcgcca ccgcgcagcc gaatttggcg cgcttcgggc acttggcctt
     1441 gaggcccggg taggcgacgg tcacggtctt ggtgatcgtc gccgccgggt tgccgttgca
     1501 gtaggccgtc tgtccggtga agacggtcgc gaccgagcag ccctcgttgt cggtgacggt
     1561 cagcgtcacg ctgtacttcc ctggtttggg gaacgagtgc cggaccttcg ggcccgcact
     1621 gccggaggct cccgtgggga aggcccacgt gtagagaccg acggtgccgt cggagtcgct
     1681 cgacccgctc gcgttgaacg ccgccgggac gccgggccgc gcccggccct gcagctggaa
     1741 cgcggcggtc ggcgactgat cggggacgat cgcgagctgc cccgcgcctt cggctttcag
     1801 gtgggctccg gtgagctggt tggtcgcgag attgaggccg gcgacaccca cgttttccga
     1861 gacgaagccg gcctggccgg tcggcgtcag ggcgacgaat tccgcgttgg ggaccccggg
     1921 gatcgtcgcc gcgacctggt tcgtccccaa gtcgacgacc gaaaccgtgt cgctgccgaa
     1981 gttgccgacg taggcggtgc gcccgttggc gctgaccgtg acgccgctcg ggtcgttgcc
     2041 gaccgggatc ggcgcgccga ccgcctgcag cgttttcgcg tcgatcgccg tcagcgatgc
     2101 gtcgtcgttg ttggcgacca gaacgcgcgc gccgtccggg gtgacggcga gaccgtaggg
     2161 attgtcgccg acggggatcg gcagcccgat cacctgcagc gttttcgcgt cgatcacagt
     2221 gacgttgtcg ttggagctgt tgggcacgaa gacacgtgag ccgtcgggtg agatcgcgac
     2281 cgcgaaggcg ttttccccga ccgggatcgg cgcgccgatc gtctgccggg tcgccaggtc
     2341 gatcaccgag acggttttgt cgcccgggtt ggagacgaag gcgcggctcc cgtccggggt
     2401 gatcgcgatc ccgaagggcg atttcccgac cgtgatcgag ccgaccaccg ccttggtccg
     2461 ggtgtcgatc acgccgaccg tgccgttgtc ttcgttggtc gtgtagacga aattgccatc
     2521 gggggagatc gccagcgtgt agggggcgct gtcgacgccg gtcgcgatcg gcgcaccgat
     2581 ctgcgccccg gtcgtcgcgt cgagcacgaa gacggcgtcc gcttcgtagt cgggcacgta
     2641 gacctcgcgg gccgcgaacc cggtcgtggc ggtggcgagc agggcgagcg tcgcgacggt
     2701 ggcgacgagc gagatgcgct tcaaggcgat cctcctggct ggacgggctg acgagcggcc
     2761 gagccgcgcc agtgtacgtc agcccgcgcg gggcggaatc acgaactggg tgacgaccag
     2821 gttggcggcg agcttggtct cgccggcccg ctcacgctcg accttgacgt cgaggacgac
     2881 ggtgttgcgg ccgctgtgca gtggttcggc aaccgcggtg agcacgtcgc cgtcgaggcc
     2941 ggagccgaac acgttcagct tcagctcgat cgtcgtgaag tcgtggtagg gcggcatcgc
     3001 ccggaaggtc gcccaggcgg cggcggtgtc gccgagcgcc gtccagacgc cgccgtggac
     3061 gtagcccccc gggtgcagat ggcggcgatc gacgacgatc cgcgcaacgg tgcggtcgtc
     3121 gccgatctcc agcggttcga tccccagatc gcccggaaag ctgccgccga tgaagccgcg
     3181 gatgtgctcc gggctgatct gggagaaatc gattccgcgc ggctcgttca ctcgctgacg
     3241 ctcagatgcg ctcgacgatc atcgcctcgc cctgaccgcc ggcgacgcac atcgtctcga
     3301 ggccgacctg gtggtcgtcg gtctccatca cgttgagcag ggtgcccatg atccgggcgc
     3361 cggtcatccc gaaggggtgg ccgagggcga tcgcgccacc gtgcgtgttc atcttctcca
     3421 gcgggatgtc gcattcgtcc atgatcggga tcacctgggc ggcgaacgcc tcgttcagct
     3481 cgaccgcgcc gacgtcgtcg atcgtcatcc cggcccggtc cagcaccttc ttgatcgcgc
     3541 cgatcggggc gacgcccatc agctccggct cgttgccgtg cgtggcggcg gtgacgatcc
     3601 gggcgcgcgg gctgaggccg agctccttgg ccttggtgtc gctcatgatc aggaccgcgg
     3661 cggcgccgtc gttgagcggg caggagttgc cggcggtgac cccgccgccg ccgaaggcct
     3721 ccggcagttc ggagagcttc tccagggtcg agctggcgcg cgggccgtcg tccttggcga
     3781 cctcggtgcc gtcgggcagg gtcacgggga cgatctcgcg gtcgaagaac ccgtcctcct
     3841 gcgatttcac cgccagttcc tgcgagcgct gcgcgtactt gtccatgtcg gcgcggctga
     3901 cctcgtactt gttcgccacg ttgacggcgg tgatgcccat gtcgatgtag gcgttgggct
     3961 ggccgttctc gccgatcagg ttcgggttgc ggtcgtcgac cccggcggcc tcggtgcgct
     4021 cgttgtagcg gctcacccac tcgaccccgg cggcgatgta gacgtcgccc tcgccggcct
     4081 tgaccgcgtt ggcggcgtgg cggatcgagt cgaggctgga ggcgcagtag cgcgagacgg
     4141 tgacgccgtt gacggtctcc ggcagcttct cggagagcag cgcgatgatc cgggcgacgt
     4201 tgaacgcctg caggccctgc ggcatgccga ctccgcagaa gagatcttcg atctcagccg
     4261 ggtcgacgcc ggggttgcgc tcgagcagct ggtcgacgac gtaggcgccc atctcatcgg
     4321 ggcggagctg ggctagcgac cccttgaagg cacggccgat tggcgtgcgg agggtgtcga
     4381 cgatcactgc ttcaggcatt ggcgttctcc cttaaaagct gttccggttc cgaaatgcgc
     4441 gggagagcct agttgattgg ccagtcagtc aatagtgaca atacgcacat aatctccggg
     4501 tttcgtcccc aggacgcgtt acattcgcct caatccagct cgctgagtgc gagacaacgc
     4561 cgttccggcg gctgcgaaag gagacccttc atgcccgagg gctactgcgt caaagagcgc
     4621 aagaaggttg agatcaaaga cgccaaacag gtgacgatga agaacggtcg tccggcgatt
     4681 caggggtttt gccccgattg cgggacgaaa atcttcaaga tcggaaaact cgaggggtaa
     4741 ggccgccagc gaaccgcggc ccggcgagcg ccgggtcgct cgcggtctga acctgggtgc
     4801 gtccgctgcc gtatccgggg tcgatggtcg aactgagcag cgagcgcgat gtgcgggagg
     4861 cttacgccgc ccactccggt gagctgtacg gctttgcgct gcgctcactc ggcgacgcgg
     4921 ggctggccga agaagcggtg caggagacct tcgtgcgcgc ctggcgcgcg ggcgagcgct
     4981 tcgaccccga gatcgcctcg ctgcgcacct ggctgttcgc gatcctgcgc aacgtggtga
     5041 tcgacttcgg ccgggcgcgg gcggcgcgac cgaagctgac cagcgacgcc ggggtcgagc
     5101 cctcggtcga gccgctcgag caggcgttgc tctcctggca ggtcgaggag gcgatgcggc
     5161 ggatcggcga ggaccaccgg cgcgtgctgg tcgagaccca ctaccggggg cggccctacg
     5221 ccgaggtggc ggaggaactg ggcgtgccgg agggcacggt gaagagccgc gtctactacg
     5281 ggctgcgggc gctgcgggtc gtgctcgagg agatgggcta tgagggctga ggagcaccgc
     5341 gagctgcggc tctcgctcgg cgcctacgcg ctcggcgacc tctcgcccgg cgagcgcgcc
     5401 gcggtcgagg ggcacctgcg tgagtgcgcc gagtgccggg ccgaggccga ggcgctcgtc
     5461 tcggtcgggc ggctcctgcc gctggccgac ccggcccgct tcagcgagcc ggcgccgcgg
     5521 ccgccggccg agttgggcga gcgggtggcg gcgacgatcg gggccgagcg gcgggcggag
     5581 cgctcgcagc ggcgccggcg gcggggtttc ggcttcgcgc tcgccggagc gacggcggcc
     5641 gcggcgctgg tcctggcgct ggtcgtcttc aacggcgacg gcggcggcga ggatccggcc
     5701 acgcacgtcg agttctcctc gctgccgggc aaggtcgaga tctcggcgaa gctggtcccg
     5761 cacgcctacg gcaccgagat ccacatgtac gtgaaggggg cgcgctcggg cacgctctgc
     5821 caggtcttcc tgcgcgacca gcagggccgc cggttctccg ccggcagctt ccgctaccgc
     5881 tggggcgatg actccaacgc ggtgctcagc tccgcgctcg atctgtcccg aaccaagtcg
     5941 atcgccgtca acgccggcgg ccacacctac gtcgcgccgg tcgggcaagg acccagcgcg
     6001 gcgatctgga atcccaccga ggaggacgcc tgatgaagac gagcttgaaa accaaccgcg
     6061 tcgcgctgct gctcgccgcc ctcgccctga tcgcggcgct ggcgatcgcc ggctgcggcg
     6121 gcagcgacga cggcccggaa gcccgcggct acggcggtgg tggcggtggc ggcgccgaca
     6181 ccacgggcgg cgagatcacc gccgccccca accc
//
LOCUS       DASRIG010000283         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1960873, whole genome shotgun sequence.
ACCESSION   DASRIG010000283 DASRIG010000000
VERSION     DASRIG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960873"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFB52_07795"
     CDS             complement(<1..770)
                     /locus_tag="VFB52_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO06274.1"
                     /translation="MDKRKVLVIGGGISGASAAIELARRGFEARVVEREPVWHALGSG
                     ITLMGPALRALKRIGILDRCMETAVGVDEFRICNAASEVVQVIPLPRAEPEAAGMLGM
                     MRPNLHRILAEYAEEQGVDVRLGLSPLELRNGAEGVSVVLSDGAEEAYDLVVAADGFH
                     SSTRAAVFGEVEPVFRSQGVFRAVLPRPAEADAAFYFHGPGAQSGLTLTGADSLYMYC
                     DAAVETPARPPQEQLPELMRGHLGHLSGMAGELRDLIQ"
     gene            complement(763..1275)
                     /locus_tag="VFB52_07800"
     CDS             complement(763..1275)
                     /locus_tag="VFB52_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO06275.1"
                     /translation="MDRDARIDRLLDKQEITECILRFARGVDRHDAELARSAYHEGAR
                     DDHAMFVGSGAEVVEWADGLHDQHFRSHQHYVANVLIELDGDEAHTESYVIRVGGAKD
                     SHGHTLGGGRYLDRLERRDGEWRIVDRVLIIEWDDDFGPVADASLASSQDREDPSYTR
                     PLRVERQSDG"
     gene            complement(1352..>2077)
                     /locus_tag="VFB52_07805"
     CDS             complement(1352..>2077)
                     /locus_tag="VFB52_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZO06276.1"
                     /translation="PASPTSLNAQIKQASDKGITVTANLIPPESEDLDFAIFDSLTAE
                     AEISTAVLAEEIGEEGEVFGLFGGEGSAPNIFYVKGVLNALKKFPKIEMVYRKDFPAF
                     NPAEAQTAMEDALAAHPDVNAVITNATSLNPGVQRALQSAGLESVPTIGIGPNTKPEI
                     EGIRSGQIVSATLPAFYKSGELIIEWTAAIIDGTKPKAPLMEIPFLELSPANIEDAIS
                     TGAIYYGLTPAQLGCGPGESEEC"
BASE COUNT          292 a          709 c          724 g          352 t
ORIGIN      
        1 tcctggatca ggtcgcgcag ctcccccgcc atccccgaga ggtgcccgag gtggccgcgc
       61 atcagctccg gcagctgctc ctgcggcggg cgggccggcg tctcgaccgc ggcgtcgcag
      121 tacatgtaga gcgagtcggc cccggtcagc gtcagtcccg actgggcgcc gggaccgtgg
      181 aagtagaacg ccgcgtcggc ctccgccggg cgcggcagca ccgcccggaa gacgccctgg
      241 ctgcggaaga ccggctcgac ctcgccgaac acggcggccc gggtcgagga gtggaagccg
      301 tcggcggcga cgaccaggtc gtaggcctcc tcggcgccgt cgctgagcac gacgctgacg
      361 ccctccgccc cgttgcgcag ctccagcggg gacaagccga gccgcacgtc gaccccctgc
      421 tcctcggcgt actcggcgag gatccggtgc aggttcgggc gcatcatccc cagcatcccc
      481 gcggcctcgg gctcagcccg cggcagcggg atcacctgca cgacctcgga ggcggcgttg
      541 cagatcctga attcgtcgac cccgaccgcc gtctccatgc agcggtcgag gatcccgatc
      601 cgcttcagcg cccgcagcgc cgggcccatc agcgtgatcc cgctgccgag cgcgtgccag
      661 accggctcgc gctcgaccac ccgggcctcg aagccgcgcc gggcaagctc gatcgcggcc
      721 gaggcgccgg agatgccgcc gccgattaca agcactttcc gcttatccat ctgactgcct
      781 ttcgacacgt aggggacggg tgtaagaggg gtcctcgcgg tcctgggaag acgcgaggga
      841 agcgtcggcg accggcccga agtcgtcgtc ccattcgatg atcagaacac ggtcgacgat
      901 ccgccactcg ccgtcgcggc gctccagccg gtcgaggtag cggccgccgc cgagggtgtg
      961 gccgtgggag tccttcgctc ccccgaccct gatcacgtag ctctcggtgt gcgcctcgtc
     1021 gccgtcgagc tcgatcagga cgttggcgac gtagtgctgg tgggaacgga agtgctggtc
     1081 gtgcaggccg tcggcccact cgaccacctc cgccccggag ccgacgaaca tcgcgtggtc
     1141 gtcgcgggcg ccctcgtggt acgcggagcg cgccagctcg gcgtcgtggc ggtcgacgcc
     1201 gcgggcgaag cgcaggatgc actcggtgat ctcctgcttg tcgagcagcc gatcgatacg
     1261 ggcgtcgcgg tccatcagtt cctcctccgg gaggctcgac cgcccccctt ccccgcgggg
     1321 aaggggggcc tggtcagcgc gggatgcggg ctcagcattc ctcagactcg cccggcccac
     1381 aaccaagctg ggcgggggtc aggccgtagt agatggcgcc ggtcgagatc gcatcctcga
     1441 tgttggcggg gctgagctcg aggaacggga tttccatcaa cggtgctttc ggtttcgtgc
     1501 cgtcgatgat cgccgccgtc cattcgatga tcagctcgcc ggatttgtag aacgccggca
     1561 acgtcgccga gacgatctgg cccgaccgga tcccttcgat ttccggcttg gtgttggggc
     1621 cgatgccgat cgtcgggacg ctttcgagcc cggccgactg cagggcgcgc tggacgccgg
     1681 ggttcagcga ggtcgcgttc gtgatcaccg cgttgacatc cgggtgggcg gcgagcgcgt
     1741 cctccatcgc cgtctgcgct tcggccgggt tgaaggccgg gaagtccttg cggtagacca
     1801 tctcgatctt cgggaatttc ttcagcgcgt tgagcacgcc tttgacgtag aagatgttcg
     1861 gagcgctgcc ttcgcctccg aacaggccga agacttcgcc ttcttcgccg atttcttcgg
     1921 ccaggaccgc ggtgctgatt tcggcttccg cggtcagcga gtcgaagatc gcgaagtcga
     1981 ggtcttccga ttcaggcggg atcaggttgg cggtgacggt gatgcctttg tcgctcgcct
     2041 gcttgatctg cgcgttgagc gaggtcggcg aggcggg
//
LOCUS       DASRIG010000284         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2214619, whole genome shotgun sequence.
ACCESSION   DASRIG010000284 DASRIG010000000
VERSION     DASRIG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214619"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFB52_07810"
     CDS             complement(<1..754)
                     /locus_tag="VFB52_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO06277.1"
                     /translation="MQPTFVRKWLVCGLFALALMAVVAGCGSDDSSSDSTAETATTQE
                     SPDTTTDEVVGELEDELPQGPNPVGKAAGKTIGFVSACQNCEPMARMSAAYEEAAAAA
                     GMDVRIFDTKGNPADAAKGVETLLQAGADAIVLAALPPQVVGAAAEAAKAKDVPIYAT
                     FPGVLYKDSEGITAFNIEENTPEAQQALAERLVADVGPEAKVLYMFDEILPVGALYDA
                     GIREGLGKAEILDEAQVDLTKITEAGFDTTRTW"
     gene            complement(954..1733)
                     /locus_tag="VFB52_07815"
     CDS             complement(954..1733)
                     /locus_tag="VFB52_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZO06278.1"
                     /translation="MADEGPSPKRVRRPAKMGEMIADAIADEILEQNLQPGTRLPNEA
                     QMVERYEAGRGTVREAIRLLEADGMIDVRPGLGGGPVVREPDVERVARRLSILLRLSG
                     STFGAVVDARKALEPSLAQHAATAATDEQLEELRGSVERLASAEDAGGQVFIEENARF
                     HSLVAEASHNPVLQTFWLAIRAIVDGQEVGVRYGEGARHAVVLAHQRVLAAIEARDPE
                     LAAVEMGKHVAAIDLHLAEHHPDLIEDQIRLSGSQRSGASS"
     gene            complement(1726..2949)
                     /locus_tag="VFB52_07820"
     CDS             complement(1726..2949)
                     /locus_tag="VFB52_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06279.1"
                     /translation="MDFTLTDEQAAIREVARDFAKRQIAPNVIEWERTGHFPREVAEQ
                     MGELGFLGCCFPEEYGGSEAGFVSLVLVIEEIARVWQPAAGFFNPNGMTVPFTILNWG
                     TEEQRQRWVEPLIKGKLIGSFGLTEPEGGSDALGSMRSRATKIDGGWKLDGTKIFNSL
                     AHVADLDLVFARTSDDGHRGISAFLVPLDTPGITANPIEHSILGECIPTSEVSFADVE
                     LPEEALLGEVGQGFTIAMNALDYGRLTVPARCVGIAQGCVDVSLEYARERVVFGNPIG
                     TYQLIQGLITDMVVETDAARMLTYHSAWLKDCGKTATRESARSKYFAAEVAARVATRA
                     FELFGGYAVTDEYPVARMFTWAHLYRTGEGSANIQRMLVANDALGFKLADRHAIERTF
                     GLASDIEPVGQWLNG"
     gene            complement(2951..>3424)
                     /locus_tag="VFB52_07825"
     CDS             complement(2951..>3424)
                     /locus_tag="VFB52_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO06280.1"
                     /translation="IGGYALGGGCELALACDFRILSESAIVGLPEARIGAFPGAGGGS
                     LLTRIAGPAVAKDLIFTGRKLEAIEAERLGIASRLVPDDALDEETAALAACIAANGPL
                     GVRFAKQAIDRGGDGPIATSFAFEVAAGQVVLGSEDYLEGLASFAERRPAVFRGR"
BASE COUNT          496 a         1156 c         1222 g          550 t
ORIGIN      
        1 gccaggtccg ggtggtgtcg aagccggctt cggtgatttt ggtcaggtcg acctgggctt
       61 cgtcgaggat ctcggctttg ccgaggccct cgcggatgcc ggcgtcatac agcgccccga
      121 ccgggaggat ctcgtcgaac atgtagagga ctttcgcttc cgggccgacg tcggcaacca
      181 gccgttcggc cagggcctgc tgcgcttccg gggtgttctc ctcgatgttg aaggcggtga
      241 tgccttccga gtctttgtag aggacgcccg ggaaggtggc gtagatcggc acgtctttgg
      301 ctttcgcggc ttcggccgcg gcgccgacta cctgcggcgg cagcgcggcg agcacgatcg
      361 cgtcggcgcc ggcctgcagc agcgtttcca cgcctttggc cgcgtcagcc gggttgccct
      421 tggtgtcgaa gatccgcacg tccatgccag cggcggccgc ggcctcttcg taggcggcac
      481 tcatgcgcgc catcggctca cagttctggc aggcgctgac gaaaccgatc gttttgccgg
      541 cggctttgcc gactgggttg ggaccctgcg gcagttcgtc ttcgagctcc ccgacgacct
      601 cgtcggtggt ggtgtccggt gactcctgcg tcgtcgccgt ttccgcggtc gaatcgctcg
      661 acgagtcgtc gctaccgcac ccggccacga cagccatcag cgcaagcgca aacaggccgc
      721 agaccagcca cttcctcaca aacgtgggct gcacgtcttc tcctccttag tagtgacgcg
      781 cccggaatgg acgcgtcgaa ctccataggt acatactata cgcatgttta ggcgggaagt
      841 caatgcaaac cgcatcgaca agccattggg gcttggaaaa cgccgcgtgg ttccttaccg
      901 gcccataggt ccggaaagga accgcgcgat cggctggcgg tagccgatcg cggctaggag
      961 gaggcgccgg agcgctggga accgctgagg cggatctgat cctcgatcag gtccgggtga
     1021 tgctcggcga ggtgaaggtc gatcgcggcg acgtgcttgc ccatctcgac ggcggcgagc
     1081 tcggggtcgc gggcctcgat cgcggccagc acgcgctggt gggcgaggac gaccgcgtgg
     1141 cgggcgccct cgccgtagcg gactcccacc tcctggccgt cgacgatcgc gcggatcgcc
     1201 agccagaagg tctgcaggac cgggttgtgg ctggcttcgg cgacgaggct gtggaagcgg
     1261 gcgttctcct cgatgaagac ctgcccgccg gcgtcctcgg cggacgcgag ccgctcgacc
     1321 gagccgcgca gctcctcgag ctgctcgtcg gtggcggcgg tcgccgcgtg ctgggcgagc
     1381 gagggctcga gcgctttgcg ggcgtcgacg acggcgccga aggtgctgcc ggagaggcgc
     1441 aggaggatcg agagccgccg cgccacccgt tcgacatcgg gctcgcgcac gaccggtccg
     1501 ccgccgaggc cgggacggac gtcgatcatc ccgtcggcct cgagcaggcg gatcgcctcg
     1561 cgcacggtgc cgcggccggc ttcgtagcgc tcgaccatct gcgcctcgtt ggggagacgc
     1621 gtgcccggct gcaggttctg ctcgaggatc tcgtcggcga tcgcgtcggc gatcatctcg
     1681 cccatcttgg cgggacgacg gacccgtttg gggctggggc cctcgtcagc cattcagcca
     1741 ctgaccgacg ggctcgatgt cgctggccag cccgaaggtc cgctcgatcg cgtgccggtc
     1801 ggcgagcttg aagccgagtg cgtcgttggc gaccagcatc cgctggatat tcgccgaccc
     1861 ctcccccgtc cggtacagat gcgcccaggt gaacatacgg gccacagggt actcatcggt
     1921 cacggcgtag ccaccgaaga gctcgaaggc gcgagtcgcc acccgggccg caacctcggc
     1981 ggcgaagtac ttcgagcggg cgctctcgcg ggtcgcggtt ttgccgcagt ccttcagcca
     2041 ggcgctgtgg taggtcagca tccgggccgc gtcggtctcg accaccatgt cggtgatcag
     2101 gccctggatc agctggtagg tgccgatcgg gttgccgaag acgacgcgct cgcgggcgta
     2161 ctcgagcgag acgtcgacgc agccctgagc gatcccgacg cagcgggcgg gcaccgtgag
     2221 gcggccgtag tcgagcgcgt tcatcgcgat cgtgaacccc tgcccgacct caccgagcag
     2281 cgcctcctcg ggcagctcga cgtccgcgaa ggagacctcg gaggtcggga tgcactcgcc
     2341 gaggatcgag tgctcgatcg ggttggcggt gatccccggc gtgtcgagcg ggaccaggaa
     2401 ggcggagatc ccgcggtggc cgtcgtcgga ggtgcgggcg aagacgaggt cgaggtcggc
     2461 gacgtgcgcg agcgagttga agatcttcgt gccgtcgagt ttccagcccc cgtcgatctt
     2521 ggtcgcgcgg ctgcgcatcg agccgagcgc gtcggagccg ccctcgggct cggtcagccc
     2581 gaaggagccg atcagcttgc ctttgatcag cggttcgacc cagcgctggc gctgctcctc
     2641 ggtcccccag ttgaggatcg tgaagggcac ggtcatccca ttcgggttga agaagccggc
     2701 cgccggctgc cagacgcggg cgatctcctc gatcaccagc acgaggctga cgaagccggc
     2761 ctcgctgccg ccgtactcct cggggaagca gcagccgagg aagcccagct cccccatctg
     2821 ctcggcgacc tcgcgcggga agtggccggt ccgctcccac tcgatcacgt tcggggcgat
     2881 ctggcgcttg gcgaagtcgc gggcgacttc gcggatcgcg gcctgctcgt cggtcagcgt
     2941 gaagtccatc tcagcgcccc cggaagacgg cgggacggcg ctcggcgaac gaggccagcc
     3001 cctccaggta gtcctcggag ccgagcacga cctgcccggc ggcaacctcg aaggcgaagc
     3061 tggtcgcgat cgggccatcg ccgccgcggt cgatcgcctg cttggcgaag cgcaccccga
     3121 gcgggccgtt ggcggcgatg caggccgcca gcgccgcggt ctcctcgtcg agcgcgtcgt
     3181 ccgggaccag ccgcgaggcg atccccagcc gctcggcctc gatcgcctcg agtttgcggc
     3241 cggtgaagat caggtccttg gcgaccgccg gcccggcgat ccgggtcaac agcgagccgc
     3301 cgccggcccc ggggaaggcg ccgatccgcg cctcggggag gccgacgatc gcgctctcgg
     3361 agaggatccg gaagtcgcag gcgagcgcca gctcgcaacc gccgccgagc gcgtagccgc
     3421 cgat
//
LOCUS       DASRIG010000285         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2214681, whole genome shotgun sequence.
ACCESSION   DASRIG010000285 DASRIG010000000
VERSION     DASRIG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214681"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFB52_07830"
     CDS             complement(<1..1280)
                     /locus_tag="VFB52_07830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZO06281.1"
                     /translation="MSEQLWGGETTKAVDNFPVSGERVPVPVVRWLGRIKGAAAEANG
                     ALGKLDADVAGQIAKAGKAVAEGEYDDQFPVDVFQTGSGTSSNMNANEVIANLAGDGA
                     HPNDHVNMGQSSNDVFPSAVHLAALDEVTHDLLPAMGKLQSALEAKATEFADVVKAGR
                     THLMDAVPVTLGQEFGGYAAQVRLGVQRVEATLRRVGQIPLGGTATGTGLNTHPEFAA
                     KVRETLAKDTGLAISEPLDPFEAQGNRDALVELSGALKVYAVSLNKIANDLALMGSGP
                     RAGLAELRLPELQKGSSIMPGKVNPVIPEVVIQVAGQVIGNDAAITFAGSQGQFELNV
                     RVPLIARNLLDSVKLLASASRLLNERCVAGLEANEEMTERHAEATLATATALNPHIGY
                     DRAADIVKAAAESGRSLREVALEQGVDPAVLEEAL"
     gene            1440..2072
                     /gene="rpsD"
                     /locus_tag="VFB52_07835"
     CDS             1440..2072
                     /gene="rpsD"
                     /locus_tag="VFB52_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZO06282.1"
                     /translation="MGRYIGPVEKLERREGVSLDLKGKRQLRGKTSLERRGAQPPGQH
                     GAARRRQPSVYGQQLREAQKLKTAYGIREKQMRRLVDKARRNRETTSGEALLEMLERR
                     LDNIVFRLGLAATRRQARQFVTHGHVQVDEKKLDIPSAQISDGSWIRIAADSPVRDVA
                     REATEDVGLTPAWLVADHDALAGKVLRAPERAEIAMPVAEQLVIERYARR"
     gene            2154..2465
                     /locus_tag="VFB52_07840"
     CDS             2154..2465
                     /locus_tag="VFB52_07840"
                     /inference="COORDINATES: protein motif:HMM:NF022083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZO06283.1"
                     /translation="MKPDDPVSAGAERAIEHLNEDHPDALLEMAQALGGQPEATAARC
                     VDADRTGLDLVATTPAGETPVRVSYEEPIEEPAGLRKATVKLAQKARAELGREARPVS
                     P"
     gene            2485..3099
                     /locus_tag="VFB52_07845"
     CDS             2485..3099
                     /locus_tag="VFB52_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06284.1"
                     /translation="MSAKLRESTAVRLLASPGLQHDQAQAALAASFSVTAISSPGELL
                     DRASLPLFGAGEAEVHEQAHALADQLTNGLCLNPDRQHHRALLIDHALTHRSAHPWQI
                     AVIGHELARRAGIASFVGLCEGEPWTVVRGEGQMALVGPCSISGRPDASQVRPRCAHQ
                     VARAVLTEIRAAAPTESADRAARLLRALPCGGRGCRRRRGGEAD"
BASE COUNT          452 a         1120 c         1118 g          420 t
ORIGIN      
        1 agcgcctctt cgaggaccgc ggggtctacg ccctgctcca gcgctacctc gcgcagggag
       61 cggcccgatt cggccgccgc cttgactatg tccgccgcgc ggtcgtagcc gatgtgggga
      121 ttgagggcgg tcgccgtcgc cagcgtcgcc tcggcgtggc gctcggtcat ctcctcgttc
      181 gcctcgagac cggcgacgca cctctcgttc agcaggcgcg aggccgaggc gagcagcttg
      241 accgagtcga gcaggttgcg ggcgatcagc ggcacccgca cgttgagctc gaactggccc
      301 tgggaaccgg cgaaggtgat cgcggcgtcg ttgccgatca cctggccggc gacctggatc
      361 acgacctccg ggatcaccgg gttgaccttg ccgggcatga tcgaggagcc cttctgcagc
      421 tccggcaggc gcagctcggc gaggccggca cgcggccccg agcccatcag cgccaggtcg
      481 ttggcgatct tgttgagcga gaccgcgtag accttgagcg cgccagagag ctcgaccagc
      541 gcgtcgcggt tgccctgcgc ctcgaacggg tcgagcggct cggagatcgc caggccggtg
      601 tccttggcga gcgtctcgcg caccttggcg gcgaactcgg ggtgggtgtt gagcccggtg
      661 ccggtggcgg tgccgccgag cgggatctgg ccgacccggc gcagcgtcgc ctcgacccgc
      721 tggaccccga gccggacctg cgcggcgtag ccgccgaact cctggccgag cgtgaccggg
      781 acggcgtcca tcaggtgcgt gcggccggcc ttgacgacgt cggcgaactc ggtcgccttc
      841 gcctccagcg ccgactgcag cttgcccatc gccggcagca ggtcgtgcgt gacctcgtcc
      901 agcgccgcca ggtgcacggc ggaggggaag acgtcgttcg aggactggcc catgttcacg
      961 tggtcgttgg gatgggcgcc gtcgccggcg aggttggcga tcacctcgtt ggcgttcatg
     1021 ttcgacgagg tgcccgagcc ggtctggaag acgtcgaccg ggaactggtc gtcgtactcg
     1081 ccttcagcga ccgccttgcc ggccttggcg atctgcccgg cgacgtcggc gtcgagcttg
     1141 ccgagcgcac cgttggcctc ggccgcggcg cccttgatcc gccccagcca gcgcacgacc
     1201 ggaaccggca cgcgctcgcc cgagacgggg aagttgtcga ctgccttggt ggtctcgcca
     1261 ccccacagct gctcgctcaa agtgatgcct ccagaagttt gtttgggggt ttgggagggt
     1321 attcctccgg cgccgtccgc ggctctacgc cgcggtccgg aacggaatcc aggtggctct
     1381 ccgccgtccc tttacctact tattaggcgc ccctaagcga gatgtattag ggttacctaa
     1441 tgggacggta catcggacct gttgagaagc tggagcgacg cgagggcgtc tcgctcgacc
     1501 tgaagggcaa acgccagctg cgcggcaaga cctcgctgga gcggcgcggc gcgcaaccgc
     1561 ccggtcagca cggggcagcg cggcggcggc agccgagcgt ctacggccag cagctgcgcg
     1621 aggcgcagaa actgaagacc gcctacggga tccgcgagaa acagatgcgg cggctggtcg
     1681 acaaagcgcg gcgcaaccgc gagaccacct ccggcgaggc actgctggag atgctcgagc
     1741 ggcggctcga caacatcgtc ttccggctcg ggctggcggc gacccggcgc caggcgcgcc
     1801 agttcgtcac ccacggccac gtccaggtcg atgaaaagaa gctcgacatc ccctcggcgc
     1861 agatctccga cggctcctgg atccggatcg ccgccgactc gccggtccgc gacgtcgccc
     1921 gcgaagcgac cgaggacgtc ggcctgaccc cggcctggct ggtcgccgac cacgacgccc
     1981 tcgcgggcaa agtgctgcgg gcgcccgagc gcgcggagat cgcgatgccg gttgccgagc
     2041 agctcgtgat cgaacgctac gcgcgccgct gagccgcccg atcccgaccg acccacccac
     2101 gaacttacgg ccaggcaccc agagatgaac gcaaccgaca cgaaggcagg gagatgaagc
     2161 cagacgatcc ggtcagcgcc ggcgccgagc gcgcgatcga gcacctcaac gaggaccacc
     2221 ccgacgcatt gctggagatg gcgcaggcgc tcggcggcca gccggaggcg acggcggcgc
     2281 gctgcgtcga cgccgaccgc accgggctcg acctggtggc tacgacgccg gcgggggaga
     2341 ccccggtccg ggtctcctac gaagagccga tcgaggagcc ggcggggctg cgcaaagcga
     2401 ccgtcaagct cgcccagaag gcccgcgccg agctcgggcg cgaggcgcga ccggtctctc
     2461 cgtagggcag ggggcgcgag cgggatgagc gccaaactga gggagagcac ggcggtgcgg
     2521 ctgctcgcct cgccgggcct ccagcacgac caggcccagg cggcgctggc ggcgagcttc
     2581 tcggtgaccg cgatctcctc gcccggggag ctgctcgacc gcgcctcgct gccgctgttc
     2641 ggggccggcg aagccgaggt ccacgagcag gcgcacgcgc tcgccgacca gctgaccaac
     2701 gggctctgcc tcaaccccga ccgccagcac caccgggcgc tgctgatcga ccacgcgctc
     2761 acccaccgca gcgcccaccc gtggcagatc gccgtgatcg gccacgagct ggcgcgccgc
     2821 gccggcatcg ccagcttcgt cggcctctgc gagggcgagc cctggaccgt ggtccgcggg
     2881 gaggggcaga tggcgctggt cgggccctgc tcgatctccg gccgccccga cgccagccag
     2941 gtgcgccccc gctgcgcgca ccaggtcgcg cgcgcggtgc tgaccgagat ccgcgcggcc
     3001 gccccgaccg agtcggccga ccgcgccgcc cgcctcttgc gggcgctgcc ctgcggcggt
     3061 cgcggctgcc ggcggcgccg cggcggcgag gcggactagc gtcccgcgct
//
LOCUS       DASRIG010000286         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1707257, whole genome shotgun sequence.
ACCESSION   DASRIG010000286 DASRIG010000000
VERSION     DASRIG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707257"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFB52_07850"
     CDS             complement(<1..203)
                     /locus_tag="VFB52_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06285.1"
                     /translation="MATWTTSIESTKVDGTHTARGYAAAAPDAALAPHSFERRALGPH
                     DVRIVILHCGVCHSDLHMARDEWG"
     gene            complement(215..1444)
                     /locus_tag="VFB52_07855"
     CDS             complement(215..1444)
                     /locus_tag="VFB52_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06286.1"
                     /translation="MSVQLTQQAISPEDLLEPGSPRLAALLEAVAAGAAERDRDRVPP
                     YDQIQLIKDSRLGALRVPPEYGGAGGSLRDLFRVVVALGAADSNVAHILRQHFAFVEQ
                     SLRSTNEEQRERWLREAAAGTIFGGAATELGNTRVGAESVSTHLTRDAAGELRINGTK
                     YYSTGSLYSDWLMLTAADEQGEIAAVIVPADREGVTLEDDWDGIGQSLTGTGTTRLEN
                     VLVEPDEVLELRMRADGSFQRCGVLQLFITAVIAGVIRNAADDAAALVRSREGRPFAH
                     APAERPADDPLLQEVVGEMASAAYAAEATVLIAAEAQDAEAQSVLDGQPDEDLAHQAS
                     LEAAKAKVVVDEIAFRATTRIFDVGGASATKKAKNLDRHWRNARTLSTHNPLLYKAQA
                     IGDLEINGAPLPSTWFF"
     gene            complement(1459..2853)
                     /locus_tag="VFB52_07860"
     CDS             complement(1459..2853)
                     /locus_tag="VFB52_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO06287.1"
                     /translation="MSPQPILVNAFDMNCPTHLVAGTWRDPESDAAGYKDIAYWTRLA
                     QICERGLFDGIFIADVLGIYDVYGGGNDGALRGGAQVPVNDPLLVVPAMAAVTDHLGF
                     GITCSTSFEHPYTFARRMSTLDHLTKGRVGWNIVTSYLESGARNIGLDSQVRHDNRYD
                     IADEYMEVLYKLWEGSWEDDAVVEDVARNLYVDPEKVHPIEHQGEYFKVPGIHLCEPS
                     PQRTPLLYQAGQSNRGKTFAAEHAEAVFMGAPTAEIMRGNVDDLRGRAVGAGRDGHDI
                     KVFSSYSVVTAPSEAEAEEKHAYYRSLIDPEGTLVLWSGWLGFDLSPYELDEPLRMVP
                     NEAMQSTNETFGDGKWTVRDLVEGLGLGADGPTAVGSPEQVADQIEAWIDETGADGLN
                     LNHVVTPGTYADFADLVVPELQRRGRYRTSYEEGTLREKLFGRGARLPETHQAARRRH
                     DHAAAPATPAAAAA"
     gene            complement(2850..4559)
                     /locus_tag="VFB52_07865"
     CDS             complement(2850..4559)
                     /locus_tag="VFB52_07865"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06288.1"
                     /translation="MSANPPNGADANGNDPLLRVEKLSVGFGPRDKVNSVVHGISFGV
                     DAGQCLAIVGESGSGKSVTARTLVGLTGARSHVDAARIDLGGKSLLGLSDRRWRALRG
                     AEVGFVLQDALVSLDPLRKVGKEIAEPLRVHDWGDRNKREDRVIELLREVGVPEPELR
                     ARQLPGELSGGLRQRALIASAIALDPSLLIADEPTTALDVTVQAQILALLEATKTRGR
                     GLILISHDLAVVSRLADRVAVMRLGEIVEQGPVEEVLRAPKHPYTKALLDAVPAAHSK
                     GTRLAPRADGAYLAAGEPQVPALVGDREPIITAEDLVKTYRGPDGVDRAAVDHVSLEI
                     RPGETLGIVGESGSGKTTTARIVLALIDPDKGNVTLRGKPWTTLKEKDRRQRRRRISV
                     VYQDPLSSFDPRWTVAKIIDDAIATEDYKGKQRRSKRVRELLGHVGIGEELLGRRPLQ
                     LSGGQRQRVAIARALAPEPDLIVCDEPVSALDVSIQAQVLDLLSDLQAELGIAYLFIS
                     HDLGVIYHVSDRVLVMKDGRVVEQGSAEEIFRNPREAYTQQLLAALPRLDAPRPAESE
                     VAV"
     gene            complement(4559..5449)
                     /locus_tag="VFB52_07870"
     CDS             complement(4559..5449)
                     /locus_tag="VFB52_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06289.1"
                     /translation="MATTADPTLIQGPFPGGAGAGSRISSRVRALRPAVILAALYLIV
                     IAIAAIAPSLLTSRDPLEVNLGASLQSPSIEFPFGTDQSGRSVYTRVVYGARESVLIG
                     LGATAVGLLIATALGVAAALGGKVVDGGINRLLEVLFSFPGLLLALLLVAVFGSGAST
                     QILAVGIASAPGYARMIRGQVLQVKNADYVIASRALGHPPGRTLFRTILPNAFRPLVV
                     LATLGVGQAIVWASALSFLGLGVAPPAPEWGAMLDAGRDYIQTAWWLDFFPGAAIVAF
                     TLAATCLGRYFQRRIEGETD"
     gene            complement(5449..>6062)
                     /locus_tag="VFB52_07875"
     CDS             complement(5449..>6062)
                     /locus_tag="VFB52_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06290.1"
                     /translation="LFAWIIAVASTLISAGHGRLRSSVGSGLETFFAGLPQYWLGLVL
                     LVVFAFELGWFPVTGGTSLDALILPALTLALPLAGFIGQVTRDEFERGLRQPFALSAR
                     ARGVSEKEVRWRHVLRHALLPGITLSGWAVGATISGAVIVEVIFARPGLGRILVDAVS
                     AQDLPVVVGITLLLAAVYVVVNLLVDLLYVIVDPRMRSSMAVA"
BASE COUNT          849 a         2067 c         2282 g          864 t
ORIGIN      
        1 ccccactcgt cgcgggccat gtgcaggtcg gagtggcaga cgccgcagtg gaggatcacg
       61 atgcggacgt cgtgcgggcc gagcgcgcgg cgctcgaacg agtgcggggc gagcgccgcg
      121 tcgggcgccg cggcggcata gccgcgcgcg gtgtgagtgc cgtccacctt ggtcgactcg
      181 atcgacgtgg tccaggttgc catcggcccg ctccctagaa gaaccaggtc gagggcagcg
      241 gcgccccgtt gatctcgagg tcgccgatcg cctgcgcctt gtagaggagg gggttgtggg
      301 tcgagagcgt gcgcgcgttg cgccagtggc ggtcgaggtt cttcgccttc ttggtcgccg
      361 aggcgccgcc gacgtcgaag atccgcgtgg tcgcgcggaa ggcgatctcg tcgaccacca
      421 ccttcgcctt cgccgcctcc agcgaggcct ggtgggcgag gtcctcgtcg ggctgcccgt
      481 cgaggaccga ctgcgcctcg gcgtcctgcg cctcggcggc gatcagcacc gtcgcctcgg
      541 cggcgtaggc ggcgctcgcc atctcgccga ccacctcctg caacagcgga tcgtcggccg
      601 gacgctcggc cggggcgtgc gcgaagggtc ggccctcgcg gctgcggacc agggccgcgg
      661 cgtcgtcggc ggcgttgcgg atcaccccgg cgatcaccgc ggtgatgaac agctgcagga
      721 cgccgcagcg ctggaaggat ccgtcggcgc gcatgcgcag ctccagcacc tcgtcgggct
      781 cgaccaggac gttctccagg cgggtcgtcc cggtgccggt caggctctgg ccgatcccgt
      841 cccagtcgtc ctccagggtc acgccctcgc ggtcggcggg cacgatcacg gcggcgatct
      901 cgccctgctc gtcggcggcg gtcagcatca gccagtccga gtagaggctg ccggtcgagt
      961 agtacttggt gccgttgatc cgcagctcgc ccgcggcgtc gcgggtcagg tgggtggaga
     1021 ccgattcggc cccgaccctg gtgttgccga gctcggtggc ggcgccgccg aagatcgtgc
     1081 ccgccgccgc ctcgcgcaac cagcgctcgc gctgctcctc gttcgtggaa cgcagagact
     1141 gctcgacgaa ggcgaagtgc tggcgcagga tgtgggcgac attggagtcg gcggccccga
     1201 gcgcgacgac gacgcggaag aggtcgcgca aggagccgcc ggccccgccg tactcgggcg
     1261 ggacgcgcag ggcgccgagc cgcgagtcct tgatcaactg gatctggtcg tagggcggaa
     1321 cccggtcgcg gtcgcgctcg gcggcgccgg cggcaaccgc ctcgagcagc gccgccaggc
     1381 gcggcgagcc cggctcgagc aggtcctcgg gcgagatcgc ttgctgggtc agctggacgc
     1441 tcatctactg cctcgctttc aggccgcggc cgcggcgggc gtggccggcg cggcggcgtg
     1501 gtcgtggcgg cgacgcgccg cctggtgggt ctccggcagc cgcgccccgc ggccgaacag
     1561 cttctcccgc agggtgccct cctcataaga ggtgcggtag cggccgcgcc gctgcagctc
     1621 gggcacgacc aggtcggcga agtcggcgta ggtgcccggg gtgacgacgt ggttgaggtt
     1681 gaggccgtcg gccccggtct cgtcgatcca ggcttcgatc tggtcggcga cctgctcggg
     1741 gctgccgacc gcggtcgggc cgtcggcgcc gaggccgaga ccctcgacca ggtcgcggac
     1801 cgtccacttg ccgtcgccga aggtctcgtt ggtcgactgc atcgcctcgt tgggcaccat
     1861 gcgcagcggc tcgtcgagct cgtacggcga gaggtcgaag ccgagccagc ccgaccagag
     1921 caccagcgtc ccttccgggt cgatcaggct gcggtagtag gcgtgcttct cctcggcctc
     1981 ggcctcgctc ggcgcggtca ccacggagta gctgctgaag accttgatgt cgtgcccgtc
     2041 gcggcccgcc ccgaccgcgc gcccgcgcag gtcgtcgacg ttgccgcgca tgatctccgc
     2101 ggtcggcgcg cccatgaaca cggcctcggc gtgctcggcg gcgaaggtct tgccgcggtt
     2161 ggactgaccc gcctggtaga gcagcggcgt gcgctgcggc gagggctcgc agaggtggat
     2221 gccgggcacc ttgaagtact cgccctggtg ctcgatcggg tgcaccttct cggggtcgac
     2281 gtagaggttg cgggccacgt cctcgaccac ggcgtcgtcc tcccaggagc cctcccagag
     2341 cttgtagagc acctccatgt actcgtcggc gatgtcgtag cggttgtcgt ggcggacctg
     2401 ggagtcgagg ccgatgttgc gggcgccgct ctccaggtag gaggtgacga tgttccagcc
     2461 gacccgaccc ttggtcaggt ggtcgagcgt cgacatccgg cgggcgaagg tgtaggggtg
     2521 ctcgaacgag gtcgagcagg tgatcccgaa gccgagatgg tcggtgaccg cggccatcgc
     2581 cggcacgacc agcagcgggt cgttgaccgg gacctgggcg ccgccgcgca gggcgccgtc
     2641 gttgccgccc ccgtagacgt cgtagatccc gagcacgtcg gcgatgaaga tcccgtcgaa
     2701 gaggccgcgc tcgcagatct gcgccaggcg cgtccagtag gcgatgtcct tgtagccggc
     2761 ggcgtcgctc tcggggtcgc gccaggtgcc cgcgaccagg tgggtcgggc agttcatgtc
     2821 gaacgcgttg acgaggatcg gctgggggct catacggcaa cctcactctc cgccggacgc
     2881 ggcgcgtcca gccgtggcag cgcggccaag agctgttggg tgtaggcctc gcgggggttt
     2941 ctgaagatct cctcggcgct gccctgctcg accacgcggc cgtctttcat caccagcacg
     3001 cggtcggaga cgtggtagat cacgccgagg tcgtgggaga tgaacaggta ggcgatgccg
     3061 agctccgcct gcaggtcgga gagcaggtcg agcacctgcg cctggatcga gacgtcgagc
     3121 gccgagaccg gctcgtcgca gacgatcagg tcgggctcag gggcgagcgc gcgggcgatc
     3181 gcgacgcgct ggcgctggcc gccggagagc tgcagcggcc gacggccgag cagctcctcg
     3241 ccgatcccga cgtggccgag cagctcgcgc acgcgtttgg agcgccgctg cttgcccttg
     3301 tagtcctcgg tcgcgatcgc gtcgtcgatg atcttggcga cggtccagcg cgggtcgaag
     3361 gagctcagtg ggtcctggta gacgaccgag atccggcgcc ggcgctggcg ccggtccttc
     3421 tccttcagcg tcgtccaggg cttgccgcgc agggtgacgt tgcctttgtc ggggtcgatc
     3481 agggcgagca cgatgcgcgc cgtcgtcgtc ttgcccgagc ccgactcgcc gacgatcccg
     3541 agcgtctcgc ccgggcggat ctcgagcgag acgtggtcga ctgcggcgcg gtcgacgccg
     3601 tcggggccgc ggtaggtctt gaccaggtcc tcggcggtga tgatcggctc gcggtcgccg
     3661 accagggccg gcacctgcgg ctcgccggcg gcgaggtagg cgccgtccgc ccgcggggcc
     3721 agccgcgtgc ccttcgagtg ggcggcgggg accgcgtcga gcagggcctt ggtgtacgga
     3781 tgcttcggag cccggagcac ctcctcgacc ggaccctgct cgacgatctc gccgagccgc
     3841 atgaccgcga cccggtcggc cagccgcgag acgaccgcga ggtcatggct gatcaggatc
     3901 aggccgcggc cgcgggtctt ggtcgcctcg agcagggcga ggatctgcgc ctgcacggtg
     3961 acgtcgagcg cggtggtcgg ctcgtcggcg atcagcaggc tggggtcgag ggcgatcgcc
     4021 gaggcgatca gcgcccgctg gcgcagcccg ccggagagct ccccgggcag ctggcgcgcc
     4081 cgcagctcgg gctcgggcac gccgacctcg cgcagcagct cgatcaccct gtcttcgcgc
     4141 ttatttcggt cgccccagtc gtgcacgcgc agcggctcgg cgatctcctt gccgactttg
     4201 cgcagcgggt cgagcgagac cagtgcgtcc tggagcacga acccgacctc ggccccgcgc
     4261 agcgcccgcc agcggcgatc gctgagcccg agcagcgatt tgccccccag gtcgatccgg
     4321 gcagcgtcga cgtgcgagcg ggccccggtc agcccgacca gggtgcgcgc ggtgacgctc
     4381 ttgcccgagc cggactcgcc gacgatcgcc aggcactggc cggcgtcgac gccgaaggag
     4441 atcccgtgga ccacgctgtt gaccttgtcg cgcgggccga agccgaccga cagcttctcg
     4501 acccgcagca gggggtcgtt tccgttggcg tcggcaccgt tggggggatt ggcgctcatc
     4561 agtcggtctc tccttcgatc cgtcgttgga agtagcggcc gaggcaggtg gccgcgaggg
     4621 tgaacgcgac gatcgccgcg ccggggaaga agtcgagcca ccaggccgtc tggatgtagt
     4681 cgcggccggc gtcgagcatc gccccccact ccggcgccgg cggggcgacg ccgaggccga
     4741 ggaagctgag cgccgaggcc cagacgatcg cctggccgac cccgagcgtc gccagcacga
     4801 ccagcggccg gaaggcgttc ggcaggatcg tccggaacag ggttcgcccc ggtgggtggc
     4861 cgagcgcccg cgaggcgatc acgtagtcgg cgttcttcac ctgcaggacc tggccgcgga
     4921 tcatccgcgc gtagcccggc gccgaggcga tcccgacggc gaggatctgg gtcgacgcgc
     4981 cgctgccgaa gaccgccacc aggagcagcg ccaagagcag gccggggaag gagaagagga
     5041 cctcgagcag gcggttgatg ccgccgtcga cgaccttgcc gccgagcgcg gcggcaaccc
     5101 cgagcgcggt cgcgatcagc aggccgaccg cggtcgcgcc gagcccgatc aggaccgact
     5161 cacgggcgcc gtagacgacc cgcgtgtaga cgctgcggcc cgactggtcg gtgccgaagg
     5221 ggaattcgat cgagggcgac tgcaggctgg cgccgaggtt gacctcgagc gggtcgcggc
     5281 tggtcagcag cgagggcgcg atcgcggcga tcgcgatcac gatcaggtag agcgccgcga
     5341 ggatcaccgc cgggcgcagc gcccgtacgc ggctggagat ccgggacccc gccccggcac
     5401 cgccggggaa ggggccctgg atcagggtgg gatcagcggt ggtcgccatt acgccaccgc
     5461 catcgagctg cgcatgcgcg ggtcgacgat cacgtagagc agatcgacca gcaggttgac
     5521 gacgacgtag acggcggcga gcaggagcgt gatcccgacc acgaccggca ggtcctgggc
     5581 gctgaccgcg tcgaccagga ttcggccgag cccgggccgg gcgaagatca cctcgacgat
     5641 caccgcgccg ctgatcgtcg ccccgaccgc ccagccggag agcgtaatcc ccggcagcag
     5701 ggcgtggcgg aggacgtgtc gccagcgcac ttccttctcg ctgacgccgc gggcgcgggc
     5761 cgagagcgcg aagggctggc gcaggccgcg ctcgaactcg tcgcgggtga cctggccgat
     5821 gaaaccggcg agcggcagcg ccaaggtcag cgccggcaag atcagggcgt cgagcgaggt
     5881 gccgccggtg accgggaacc agcccagttc gaaggcgaag acgactagta agacgagccc
     5941 gagccagtac tgcggcaggc cggcgaagaa agtctcaagg cccgagccga ccgatgatcg
     6001 caagcgcccg tgccccgcgc tgatcagggt gctggcgacg gcgatgatcc aggcgaacag
     6061 ga
//
LOCUS       DASRIG010000287         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2762030, whole genome shotgun sequence.
ACCESSION   DASRIG010000287 DASRIG010000000
VERSION     DASRIG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762030"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFB52_07880"
     CDS             complement(<1..537)
                     /locus_tag="VFB52_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO06291.1"
                     /translation="MDWSYPLYCSCRRNADRVALVGRDGEVTYGELERRVAAVTAGFE
                     ELGLAGATVATLSANEPETVELYMALARAGATAVPVNTRLTTAEKRFIFEDSGVSAVV
                     ADAEFLAEARGLRDEVAAVRTVIPVRDSDDGPGLTDLRDSGPGRPAGAEDGEERAPAT
                     IIYTSGTTGFPKGVVRSHR"
     gene            634..1422
                     /locus_tag="VFB52_07885"
     CDS             634..1422
                     /locus_tag="VFB52_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO06292.1"
                     /translation="MPDADWIGLEHDDGVTVMAMARPPVNQFDTPFLQAVLDGIGGLG
                     EETRALVITSSVPGLFAAGGDIPWMASATVEEQLPFVRLCQDTYSAFERADFPTISAI
                     EGHCLGGGLELSLACDIRVASRTANLGLPETTVGLIAGAGGTQRLVRAIGQGIARDML
                     LTGRRISGEEAAAFGIVSRLVEEGEALAAATAIARQLADGPTESIQATKRLAVAASEL
                     ELEEGLARERAEWEQVRRSALTQEGLEAFAAKRKPDYDAVRRRD"
     gene            complement(1464..1622)
                     /locus_tag="VFB52_07890"
     CDS             complement(1464..1622)
                     /locus_tag="VFB52_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06293.1"
                     /translation="MSDIESDLPYWGEAASVDEVIDLEGDIADVDGEVMVLDRKISEL
                     CTVLELIC"
     gene            complement(2000..3589)
                     /locus_tag="VFB52_07895"
     CDS             complement(2000..3589)
                     /locus_tag="VFB52_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06294.1"
                     /translation="MSASEVPATLPAVLDAAAARDAAHPLLRCDGRDTTVAEMQEASH
                     GVAGALRSWGIERGDRAAIMMSNVPEFVPIWFGILRAGAVEVPVHSAHKGPLLEHILG
                     ESGARVLFCDAEFVPRLEGLDLSELERIVVRGELPGEGVPAGLAVHPLEEALAAAPAA
                     GLPQLAVGDVSCILYTSGTTGRSKGVVLTHGANLRLARANIDLMEYTAADVLYTAFPL
                     FHVNAKFTSLTSALISGARLVLDDRFSASGFWERMREERVTSFNYMGGLLAILAKQPE
                     REGDSDHVVERAYGGACPPNLWQPFEDRFGVRLHEHYGMTEIGIAIQNTRTDRRVGAI
                     GRPAPYFQVRLADDSDREVAVGEVGEIQVRPNEPDAMLLEYWRRPDATTAAFRNLWFH
                     TGDRARADEDGFIYYHDRLTDSIRRRGENVSSFELEAVVASFDGVVECAAYGVPSELG
                     EDEVMVAVVPEPGTDLDLDALLAHCEDRVARFAVPRYVRVMTELPKNSSQRIQKFKLR
                     DEGVTPDTVDRGLPKPRSAGG"
BASE COUNT          528 a         1292 c         1289 g          530 t
ORIGIN      
        1 gcggtggctg cggacgacgc ccttggggaa gccggtggtg ccggaggtgt agatgatcgt
       61 cgccggcgcg cgctcctcgc catcctcggc cccggcgggg cgtccggggc cggagtcgcg
      121 caggtcggtc aggccggggc cgtcgtcgga gtcgcgcacc gggatcaccg tgcgcaccgc
      181 cgcgacctcg tcgcgcaggc cccgcgcctc ggcaaggaac tccgcgtcgg cgacgacggc
      241 gctcaccccg gagtcctcga agatgaaccg cttctcggca gtcgtcagtc gcgtgttgac
      301 cgggaccgcg gtcgcgccgg cgcgggccag cgccatgtag agctcgaccg tctccggctc
      361 gttggcgctc agcgtggcga cggtggcgcc ggccaggccg agctcctcga agccggcggt
      421 gaccgcggcg acccgccgct ccaactcgcc gtaagtgacc tccccgtcgc gaccgaccag
      481 ggcgacccgg tcggcgttgc ggcggcatga gcagtacagg gggtaagacc agtccatttt
      541 cattctcccc gccccgccac gctacctcgg cccaggcacc cttccggcag ctttgtcgga
      601 agcgataggt ctgctcacgg cgatacttcc aagatgcccg acgcggactg gatcggcctc
      661 gagcacgacg acggcgtcac cgtgatggcg atggcgcggc cgcccgtgaa ccagttcgac
      721 acccccttcc tgcaggcggt gctcgacggc atcggcgggc tcggcgagga gacccgggcg
      781 ctggtgatca ccagctcggt cccgggcctg ttcgcggccg gcggcgacat cccctggatg
      841 gcgagcgcca cggtcgagga gcagctcccc ttcgtgcgcc tctgccagga cacctactcg
      901 gccttcgagc gcgccgactt cccgacgatc tcggcgatcg aaggccactg cctcggcggt
      961 gggctggagc tctcactcgc ctgcgacatc cgggtcgcca gccggaccgc gaacctcggc
     1021 ctaccggaga cgacggtcgg cctgatcgcc ggcgccgggg gcacccagcg cctggtccgc
     1081 gcgatcggcc agggcatcgc ccgcgacatg ctgctgaccg gccgccggat cagcggtgag
     1141 gaggcggccg cgttcggcat cgtcagccgc ctggtcgagg agggcgaggc gctcgccgca
     1201 gcgaccgcga tcgcccgcca gctcgccgac ggcccgaccg agtcgatcca ggcgaccaag
     1261 cgcctcgccg tcgccgcctc cgagctggag ctcgaagagg gcctcgcccg cgagcgcgcc
     1321 gagtgggagc aagtccgccg ctccgcgctg acgcaagagg gcctcgaagc cttcgccgcc
     1381 aagcgcaagc cggactacga cgccgtccgc cgccgcgact gatgggttga gcgcgctata
     1441 gcacccctaa gacatcgagc gcgtcaacag attagttcca agacggtgca gagttctgag
     1501 atcttccgat cgagaaccat cacttccccg tcaacgtccg cgatgtcgcc ttccaggtcg
     1561 atcacttcgt caaccgacgc ggcttcgccc caatacggta ggtccgactc gatatcgctc
     1621 atctcgctct cgagatcgtc aatctgtgcg agggcttctt cggctgcata gcgcgcgctg
     1681 gatgccgaat ctcccggtgg gccctctggc ccgactggcc ccctagctcc cgctggacct
     1741 tcctgcgcaa gcagcgtgat cccagagcca ataccggcgc tgagtacgca cacaccaatg
     1801 agcgccagaa cgaggttccg ttgcctcccc atggggggca tcctcgcctg tctcactgtg
     1861 gagggcaacc caactccatc gggacgaagc cgctccggtg tgagttatgc ccgcataccg
     1921 agcaaaactc acaccggaac gcgccaccgg ggcaacccgc acacctacct gccctgtggg
     1981 tgtgtgggtt gcccctgggt cagccgccag cagaccgcgg cttgggcagg ccgcggtcga
     2041 cggtgtccgg cgtcacgccc tcgtcccgca gcttgaactt ctggatccgc tggctggagt
     2101 tcttcgggag ctcggtcatc acgcgcacgt agcgcggcac ggcgaatctc gcgacccggt
     2161 cttcgcagtg ggcgagcagg gcgtcgaggt cgaggtcggt gccgggctcg gggacgacgg
     2221 cgaccatcac ttcgtcctcg cccagttccg agggcacgcc gtaggcggcg cactcgacga
     2281 cgccgtcgaa ggaggcgacc accgcctcca gctcgaagct ggagacgttc tcgccgcggc
     2341 ggcggatcga gtcggtgagg cggtcgtggt agtagatgaa gccgtcctcg tcggcgcggg
     2401 cgcggtcgcc ggtgtggaac cagaggttgc ggaaggcggc ggtggtcgcg tcggggcggc
     2461 gccagtactc gagcagcatc gcgtcgggct cgttcgggcg cacctggatc tccccgacct
     2521 cgccgaccgc gacctcgcgg tcggagtcgt cggcgagacg gacctggaag tagggcgccg
     2581 gccggccgat cgcgccgacg cggcggtcgg tgcgggtgtt ctggatcgcg atcccgatct
     2641 cggtcatccc gtagtgctcg tgcaggcgca cgccgaagcg gtcttcgaac ggctgccaga
     2701 ggttcggcgg gcaggcgccg ccgtaggcgc gctcgaccac gtggtcgctg tcgccctcgc
     2761 gctcgggctg cttggcgagg atcgcgagca ggccgcccat gtagttgaac gaggtcaccc
     2821 gctcctcgcg catccgctcc caaaagccgc tggcgctgaa gcggtcgtcg aggacgaggc
     2881 gggcgccgct gatcaacgcc gaggtcagcg aggtgaactt ggcgttgacg tggaagagcg
     2941 ggaaggccgt gtagagcacg tccgcggccg tgtactccat caggtcgatg ttggcgcggg
     3001 cgaggcgcag gttggcgccg tgggtgagca cgaccccctt cgagcgcccg gtcgtgcccg
     3061 aggtgtagag gatgcagctg acgtcgccga ccgcgagctg cggcagcccg gccgcgggcg
     3121 cggcagcgag cgcctcctcc agcgggtgca cggcgagccc ggccggcacc ccctcccccg
     3181 gcagctcgcc gcggaccacg attcgctcca gctccgacag gtcgaggccc tccagacggg
     3241 gcacgaactc ggcgtcgcag aagagcaccc gggcaccgga ctcgccgagg atgtgttcga
     3301 gcagcggccc tttgtgggcg gagtggaccg ggacctcgac cgcgccggcg cggaggatgc
     3361 cgaaccagat cggcacgaac tccggcacgt tgctcatcat gatcgcggcc cggtcgccgc
     3421 gctcgatgcc ccaggagcgc agcgccccgg cgacgccgtg gcttgcctcc tgcatctcgg
     3481 cgacggtggt gtcgcgcccg tcgcagcgca gcagcgggtg ggctgcgtcg cgggcggcgg
     3541 cggcgtcgag caccgcgggc agcgtcgccg ggacctcgct cgcgctcatc gctgcttgcg
     3601 cggccgcagg aaggcgacgg tgacgcgccg gctggagaa
//
LOCUS       DASRIG010000288         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3264231, whole genome shotgun sequence.
ACCESSION   DASRIG010000288 DASRIG010000000
VERSION     DASRIG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264231"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1849)
                     /locus_tag="VFB52_07900"
     CDS             complement(125..1849)
                     /locus_tag="VFB52_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06295.1"
                     /translation="MVARLSLVWLLLLAAAPPQDARDRSPSDLALSPDGRWALVANAT
                     SDTVALVDLQEGRVAAEVAVGRRPTSLAWTGTTAVVTNLLSDDATVLSVAPPKLEVAA
                     TVAVGDEPRGVAISGSRALVVVSGEDALVEIDLKTRAVTRRAEAGDEPWHLAVAGDRI
                     AVSCALSQEVRVLKADTLELLFRAPMRGHNLRRVAPTPDGLWAYVPNIAERGLGTSRE
                     NIDRGWVVANRLSRVPLREEGPREAISLDPRTKAVADVEGVAVAPDGLIALTAGGTHE
                     LLLLRLPLPFVAFGGPGDHIDAPLLKDAQRFRRIALGGRPVAVAFAPGGRAVVANYLS
                     NSLQVVDAEKGELVRTVALGGPAEPSLARKGEGVFYDGQRSLHQWYSCHTCHVDGHTN
                     GSTFDTMNDGRYGNLKKTLSLRGAARTGPWTWHGWQTDFKAALVHSMKTTMQGPEPAA
                     ADVDALDAFLKTIEFVPPPGKKDDAAKRGEAVFKGRACDACHVPPDYTTDDVFVVGLE
                     DKGDRYKGFNPPSLRGVSTRGPWLHDGRARTLEDVLQKHHRPKVINDKPDLTPEELKD
                     LLAFLRSL"
BASE COUNT          305 a          739 c          802 g          329 t
ORIGIN      
        1 taatggctgg agcacgagat gccgaagaga gcgagagcga ttgagagcgt gcgcatgaag
       61 ttcctccccc tggatggcgt gatcccgctg gtccccttct taatacgccc gagccccgcg
      121 cagtttacag cgagcgcagg aaggcgagaa ggtctttgag ctcctcgggc gtgaggtcgg
      181 gcttgtcgtt gatcaccttc gggcggtgat gcttctggag cacgtcctcc agggtgcgcg
      241 cgcggccgtc gtggagccac gggccgcggg tcgagacgcc gcgcagcgag ggcggattga
      301 agcccttgta ccggtcgcct ttgtcctcca ggcccacgac gaagacgtcg tcggtcgtgt
      361 agtcgggcgg cacgtggcat gcgtcgcagg cgcggccctt gaacaccgcc tcgccgcgct
      421 tcgccgcgtc gtccttcttc cccggcggcg ggacgaactc gatcgtcttg aggaacgcgt
      481 cgagcgcgtc gacgtcggcg gccgccggct cggggccctg catcgtggtc ttcatggagt
      541 ggaccagcgc cgccttgaag tcggtctgcc acccgtgcca ggtccagggg cccgtccggg
      601 cggcgccgcg gaggctcagc gtcttcttca ggttgccgta gcggccgtcg ttcatcgtgt
      661 cgaacgtcga gccgttggtg tggccgtcga cgtggcacgt gtggcacgag taccactggt
      721 ggagcgagcg ctggccgtcg tagaacaccc cctcgccctt gcgcgcgagc gacggctccg
      781 cggggccgcc gagcgcgacg gtcctgacga gttcgccttt ctccgcgtcc acgacctgca
      841 gggagttcga gagatagttg gcgaccacgg cgcgcccgcc cggggcgaag gccacggcga
      901 ccgggcgccc gccgagcgcg atccgccgga accgctgggc gtccttgagg agcggggcgt
      961 cgatgtggtc tcccggtccg ccgaaggcga cgaacggcag gggaagccgg agcagcagga
     1021 gctcgtgggt gccgcccgcc gtgagggcga tcaggccgtc gggcgcgacc gccacccctt
     1081 ccacgtcggc cacggccttg gtgcgcgggt cgagcgagat cgcctcgcgc ggcccctcct
     1141 cgcggagcgg cacgcggctc agccggttcg ccacgaccca gccgcggtcg atgttctccc
     1201 tcgaggtccc gagcccgcgc tcggcgatgt tcggcacgta ggcccagagt ccgtccgggg
     1261 tcggcgccac gcgccggaga ttgtggccgc gcatgggcgc ccggaagaga agctccaggg
     1321 tgtcggcctt gaggacgcgg acctcctggg agagcgcgca cgacacggcg atgcggtcgc
     1381 cggccacggc caggtgccac ggctcgtcgc ccgcctccgc gcggcgggtc accgcccggg
     1441 tcttgagatc gatctcgacg agggcgtcct ctcccgaaac cacgaccagc gcgcggctcc
     1501 cggagatcgc gaccccccgc ggctcgtcgc ccaccgccac ggtggcggcg acttcgagct
     1561 tgggcggggc gacggacagc accgtcgcgt cgtccgacag caggttggtg acgacggccg
     1621 tggttccggt ccaggcgagc gaggtggggc ggcggcccac ggcgacctcg gcggcgacgc
     1681 ggccttcctg gaggtcgacg agcgccaccg tgtcggacgt cgcgttcgcc acgagggccc
     1741 accggccgtc gggcgaaagg gcgaggtcgc tcggggagcg gtccctcgcg tcctgcggcg
     1801 gggccgcggc gaggaggagg agccagacga gggagaggcg cgccaccata atcttattta
     1861 cccttgacgc cggtccttcg tcggatccat cgcgatggat ccgacgaagc gccgcgcgag
     1921 gagcgcggcg gctcgaggct ccgcctcgag cgaccggtgt caagggcaag aagaaaaggg
     1981 ggcgggcggt tccaaaccgc ccgcccccga agagctttaa gggtcgagag ggctaactag
     2041 cccgcttggg gaaccctcaa ccgaacccct ttttctcggt gaccgggact tccttgcctt
     2101 cgaggatcgc cttgagggcg ccatggacgt agcgttcctt ggcggtggac tcgtcgtccg
     2161 aatagccctt gcccg
//
LOCUS       DASRIG010000289         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3810112, whole genome shotgun sequence.
ACCESSION   DASRIG010000289 DASRIG010000000
VERSION     DASRIG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3810112"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFB52_07905"
     CDS             <1..505
                     /locus_tag="VFB52_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06296.1"
                     /translation="VADSGARYRAIFGNEAGEIATVPAIVSAQVEPPSDPGTGEPGGD
                     PLPVPPATGTTVKPKIGGVAATRKVGNSRQVTVAQLACPSGGASCKVKAPRKVTIRID
                     GQAFKVTVRVPKSIAPGASASLRVRLPKAAAERLAGQQTTLRLKVTVGTPPTQTTRTI
                     KVTLKKP"
     gene            complement(539..1705)
                     /locus_tag="VFB52_07910"
     CDS             complement(539..1705)
                     /locus_tag="VFB52_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZO06297.1"
                     /translation="MVNREALRRIVQGDLSSVRVVLGLVVIAVIFQVQEDRFLSAQNL
                     TNLMLQITTIGLISVGIVYVLLLGEIDLSVGAVSGLSAAVMAVLSVNHGWNAYLAIAA
                     AVATGMAIGLLQGSLFSRFVVPSFVVTLAGLLAWEGALLQVLGQTGSLNINDPKITGL
                     ANTFYSDTVGWIFAAVIIAAYGGVLALGFRRRVAAGLGETNPLGLIVRFVALAAITIA
                     AVAILNSDRGVPLAVMILLAFVVGMEYVAKRTSFGRHVFAVGGNSEAARRAGIHVNRV
                     RIAVFMISGSMAAIGGVMAASRLFAVNQNSGGNELLLLAIAGPVIAGTSLFGGRGSVW
                     TALLGALVIGSISNGMDLLSLSSAVKLMVTGGVLLLAVLVDAVARQQRQSSGRV"
     gene            complement(1698..2501)
                     /locus_tag="VFB52_07915"
     CDS             complement(1698..2501)
                     /locus_tag="VFB52_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06298.1"
                     /translation="MAVGTPLLELEGVSKRFGPVHALDRVDFAVRSGEVVGLVGDNGA
                     GKSTLVKAIAGIHTADAGTIRFAGKEVSISKPHDATALGIATVYQDLALCDNLDVVAN
                     LFLGREEVSPGPGKVSRQLDEVRMEHETADLLSNLAVTIPSLRSEVGTLSGGQRQQVA
                     VARSLLGDPKVVLLDEPTAALGVPQTKQVLALINRLRERDLGVVVISHNLVDVFEVAD
                     RIFVLRLGAAAGDFSAKETTEEHVIGAITGIGERNNSEQDKKAEEGSDG"
     gene            complement(2503..>2705)
                     /locus_tag="VFB52_07920"
     CDS             complement(2503..>2705)
                     /locus_tag="VFB52_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO06299.1"
                     /translation="GEEVPKDKVTEEVNNGKTDVPAVLLEPVAVVKDNIKDTVIKEGF
                     VDAGELCTGPYAAACKEVGISG"
BASE COUNT          445 a          913 c          958 g          389 t
ORIGIN      
        1 cgtggccgac tccggcgccc gctatcgggc gatcttcggc aacgaggccg gcgagatcgc
       61 cacggtcccg gcgatcgtca gcgcgcaggt cgagccgccg agcgatccgg gcaccggcga
      121 gcccggtggc gacccgctgc cggtgccgcc ggccacgggc acgaccgtga agccgaagat
      181 cggcggcgtc gccgcgaccc gcaaggtcgg caactcgcga caggtgaccg tggcgcagct
      241 cgcctgcccg agcggcggcg ccagttgcaa ggtcaaggcc ccgcgcaagg tgacgatcag
      301 gatcgacggc caggcgttca aggtcacggt ccgggtgccg aagagcatcg cgccgggcgc
      361 cagcgcgagc ctgcgggtgc ggctgccgaa ggccgccgcc gagcggctgg ccggtcagca
      421 gacgaccctg cggctgaagg tcaccgtcgg caccccgccg acccagacca cacgcacgat
      481 caaggtgacg ctgaagaaac cgtgaagtag cgaggggccg gccgatcgcc ggcccctcct
      541 acacccgccc ggagctttgg cgttgctggc gcgcgacggc gtcgacgagg acggccagca
      601 gcaggacccc gccggtgacc atcagtttca ccgcggagga gagcgagagc aggtccatcc
      661 cgttcgagat cgagccgatc accagggcgc cgagcagcgc ggtccagacc gagccgcgac
      721 cgccgaagag gctggtgccg gcgatcaccg ggccggcgat cgcgagcagc aggagttcgt
      781 tgccaccgga gttctggttg accgcgaaga gacgggacgc cgccatcacg ccgccgatcg
      841 ccgccatcga gccggagatc atgaagaccg cgatgcggac ccggttgacg tggatgccgg
      901 cgcggcgggc cgcctcggag ttgccgccga ccgcgaacac gtggcggccg aagctggtcc
      961 gcttggcgac gtactccatg ccgaccacga aggcgagcag gatcatcacc gccagcggca
     1021 cgccgcggtc ggagttgagg atcgcgacgg cggcgatcgt gatcgcggcg agagcgacga
     1081 agcggacgat cagccccagc gggttggtct cgccgaggcc ggcggcgacc cggcgccgga
     1141 agccgagcgc cagcacgccc ccgtaggcgg cgatgatcac cgcggcgaag atccagccga
     1201 ccgtgtccga gtagaaggtg ttggcgaggc cggtgatctt cgggtcgttg atgttgagcg
     1261 agccggtctg gcccagcacc tgtaagagcg cgccttccca ggccaagagc ccggctaagg
     1321 tcacgacgaa ggagggcacg acgaagcggc tgaagagcga tccctgcagg aggccgatcg
     1381 ccatcccggt cgcgaccgcg gcggcgatcg ccaggtaggc gttccagccg tggttgacgc
     1441 tgagcaccgc catcaccgcg gccgagaggc cgctgaccgc gccgaccgag aggtcgatct
     1501 cgccgagcag gaggacgtag acgatcccga ccgagatcag cccgatcgtg gtgatctgca
     1561 gcatcaggtt ggtcaggttc tgggccgaga ggaagcggtc ttcctggacc tggaagatca
     1621 ccgcgatcac gaccaggccg agcacgaccc ggaccgagga gaggtcgccc tggacgatcc
     1681 ggcgcagcgc ctcgcggtta accatcgctc ccctcctccg ctttcttgtc ctgctcggag
     1741 ttgttgcgct cgccgatgcc ggtgatcgcg ccgatcacgt gctcctcggt cgtttccttg
     1801 gcggagaagt caccggcggc ggcgccgagc cgcagcacga agatgcggtc ggcgacctcg
     1861 aagacgtcga ccaggttgtg gctgatcacg accacgccga ggtcgcgctc acgcagccgg
     1921 ttgatcaggg ccagcacctg tttggtctgc ggcacgccga gcgccgcggt cggctcgtcg
     1981 agcagcacga ctttcgggtc gccgagcagc gaacgggcga ccgcaacctg ctggcgctgg
     2041 ccgccggaga gcgtgccgac ctcgctgcgc aggctgggga tcgtcaccgc caggttcgag
     2101 agcaggtcgg cggtctcgtg ctccatccgc acctcgtcga gctgcctcga gaccttgccc
     2161 gggcccggcg agacctcctc gcggccgagg aagaggttgg cgacgacgtc gaggttgtcg
     2221 cagagcgcca ggtcctggta gacggtcgcg atcccaagcg cggtggcgtc gtgcggtttg
     2281 gagatcgaaa cctctttgcc ggcgaaccgg atcgtgccgg cgtcggcggt gtggatgccg
     2341 gcgatcgcct tcaccagcgt cgacttgccg gcgccgttgt cgccgaccag gccgacgact
     2401 tcgccggagc gaacggcgaa gtcgacccgg tccaacgcgt ggaccgggcc gaatcgcttg
     2461 ctgactccct ccaactcgag aagaggagtc ccgactgcca tgtcagccgg agatccctac
     2521 ttctttgcag gcggccgcgt atgggccggt gcagagttcg ccagcgtcca cgaacccttc
     2581 tttgatcacg gtgtctttga tgttgtcctt gacgaccgcg accggttcca gcagcaccgc
     2641 cgggacgtcg gtcttgccgt tgttcacttc ttcggtgact ttgtctttcg ggacttcttc
     2701 gccgt
//
LOCUS       DASRIG010000290         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3008964, whole genome shotgun sequence.
ACCESSION   DASRIG010000290 DASRIG010000000
VERSION     DASRIG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008964"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..883
                     /locus_tag="VFB52_07925"
     CDS             161..883
                     /locus_tag="VFB52_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZO06300.1"
                     /translation="MSSAGQEGKTIAYPGLVVPEERVAPVPRRLRGMLDDELVFDTTR
                     ALYFWDKPYYPQYNVPLEDVRGDFSEREFARVYRDGPAAGTVNFEWKGLDSWFEEDEQ
                     VFVHARNPYARVDALRSSRHLRVERDGVVLAEADAVVIVFETGLPPRHYFERTAVRFE
                     HLEPSELVTECPYKGRTSEWWSVRTGETLHADLAWSYDFPTRQLLPIAGLVAFFDERV
                     DTYVDGELLARPQTPFSERPDA"
     gene            complement(891..1163)
                     /locus_tag="VFB52_07930"
     CDS             complement(891..1163)
                     /locus_tag="VFB52_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZO06301.1"
                     /translation="MCRNIRTLHNFDPPASEEEVHASALQYVRKISGATKPSQANAEA
                     FEQAVEEIADVTARLLDRLVTTAPPKDREVEAAKAKARSEKRFAAA"
     gene            1272..2711
                     /locus_tag="VFB52_07935"
     CDS             1272..2711
                     /locus_tag="VFB52_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06302.1"
                     /translation="MTTVAEARDKRWIGLILLCSAQFVVVLDASIVNVALPTIGSALD
                     FTESSLPWVVNAYVLTFGGFLLLGGRLADLLGRRRIFMAGLILFALASLAGGLATTSG
                     QLIAARAVQGLGGALLSPAALSIVATTFKDGAERNKALGVWGAVAGSGGAAGVLLGGV
                     LTEYLGWEWVLWVNVPIGIAAAAIAPSLLAETRDENELRTFDVAGAVTITAGLSALVF
                     ALVDANEVGWGSTQTILTLAGAALLLAAFVVIELRSRAPLVPFSIFRIRTITGANVVG
                     LLVGASLFSMFFFISLYMQQVLGYSAIKAGLSYLPLAVSIILSAGIASQLVTKVGFKP
                     ILATGMVLIAAGLVWFSQISADGSFLSDILGPSLLAAVGLGFSFVPVTIAAVSGIPDH
                     EQGLASGLINTSQQVGGALGLAILAAVSSSVTGNSIDPEVLTEGFQDAFLVGAAFAVL
                     GLIATLFLIRNRDSKAHVALSQEVAVEPE"
     gene            2803..>3851
                     /locus_tag="VFB52_07940"
     CDS             2803..>3851
                     /locus_tag="VFB52_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06303.1"
                     /translation="MSFAHPSLARALAGLLAIAALGCAVLLFSDRGDAAVTGVTAVDS
                     SACGPVQYGGSGAADALIVSDLPLQGDSRRRSLQMNEAIQIVLEDAGWKAGGRNVAFQ
                     ACDDSLADTGLWSKVQCKANAKAYAANPSVLAVIGTYNSGCAENMIPILGQAPGGGLA
                     MVSPGNTLICLTQSSPTCGNGTPKSLYPARRNYARVVPNDAYQGAGLAKFAQGKGIRS
                     AFILYAAGDVTSFGQAKTFNGAAKKLGIKITGFRQWNPKAYEYKRLMNVVGATAPDAV
                     LLAGLTEQNGAQLIKDKVAALGPNDGHVKLLAPDGFAQQATIDDAGSASAGMFVSLPG
                     RVPDSLTGEGAAFVG"
BASE COUNT          546 a         1416 c         1264 g          625 t
ORIGIN      
        1 tccgctgacc ggcgccggca agcgcctgct cgacgacgaa ccgcgcgccc agctgaaggc
       61 cgaggtcagt gccaaggacc cggtttacca gcggacctcg accacgacga agaagctgcg
      121 cctgcccggc ccgtagctcc cgatgcctaa catccgtggc atgagctcgg ccggccagga
      181 ggggaagacg atcgcctacc cgggcctggt cgtgcctgag gagcgcgtcg ccccggtacc
      241 gcggcggctg cgcgggatgc tcgacgacga gttggtcttc gacacgaccc gggcgctcta
      301 cttctgggac aagccctact acccgcagta caacgtgccg ctcgaggacg tgcgcggcga
      361 cttctccgag cgcgagttcg cccgcgtcta ccgcgacggg ccggcggcgg gcacggtcaa
      421 cttcgagtgg aaggggctcg attcctggtt cgaggaggac gagcaggtct tcgtccacgc
      481 ccgcaacccc tacgcgcggg tcgacgcgct gcgctcctcc cggcacctgc gggtcgagcg
      541 cgacggcgtt gtcctcgccg aggcggacgc ggtggtgatc gtgttcgaga ccgggctgcc
      601 gccgcgccac tacttcgagc gcacggctgt gcggttcgag cacctggagc cgagcgagct
      661 ggtcaccgag tgcccttaca agggacggac cagcgagtgg tggtcggtgc ggaccggcga
      721 gacgctgcac gccgacctcg cctggagcta cgacttcccg acccggcagc tgctgccgat
      781 cgccggcctc gtcgccttct tcgacgagcg cgtcgacacc tacgtcgacg gcgagctgct
      841 ggctcgcccg cagacgccgt tctccgagcg gcccgacgcc tagctaagag ctaagcggcg
      901 gcgaagcgct tttccgagcg cgccttggcc tttgccgcct cgacctcgcg gtccttcggc
      961 ggcgccgtgg tgaccagccg gtcgagcagc cgcgcggtca cgtcggcgat ttcctcgacc
     1021 gcctgctcga acgcctcggc gttcgcctgc gagggtttgg tcgcgccgct gatcttgcgc
     1081 acgtactgca gcgcggaggc gtggacctcc tcctcgctcg cgggtgggtc gaaattgtgg
     1141 agggttctga tgtttctgca catgcgccga tcttagccgc ggaccaggcc ccggtcgacc
     1201 aggaccgcac gtttcgagaa gagccgagga agccgttgtg aaatcctccg cgagatggca
     1261 ggtacccccc gatgaccacc gtcgccgaag cccgggacaa gcgctggatc ggcctcatcc
     1321 tcctctgctc ggcccagttc gtggtcgtgc tcgacgcctc gatcgtcaac gtcgccctgc
     1381 cgacgatcgg cagcgcgctc gacttcaccg agagcagcct gccctgggtg gtcaacgcct
     1441 acgtgctcac cttcggcggt ttcctgctgc tcggcggccg gctcgccgac ctgctgggac
     1501 ggcggaggat cttcatggcc ggcctgatcc tcttcgcgct cgcctcgctg gccggcgggc
     1561 tcgccaccac ctccgggcag ctgatcgccg cccgcgcggt ccagggcctc ggcggcgccc
     1621 tcctctcccc cgccgcgctc tcgatcgtcg ccacgacctt caaagacggc gccgagcgca
     1681 acaaggcgct gggggtctgg ggagccgtcg ccggctccgg cggcgccgcc ggcgtcctcc
     1741 tcggcggcgt cctcaccgaa tacctcggct gggagtgggt gctctgggtc aatgtcccga
     1801 tcgggatcgc cgccgccgcg atcgccccga gcctgctggc cgaaacccgc gacgagaacg
     1861 agctccgcac cttcgacgtc gccggcgcgg tgacgatcac cgccggcctc tcggcgctgg
     1921 tcttcgccct cgtcgacgcc aacgaggtcg gctggggctc gacccagacg atcctcaccc
     1981 tcgccggcgc ggcgctgctg ctcgccgcct tcgtcgtgat cgagctgcgc tcgcgggcgc
     2041 cgctggtccc cttctcgatc ttccgcatcc gcacgatcac cggcgccaac gtggtcgggc
     2101 tgctggtcgg cgcctcgctg ttctcgatgt tcttcttcat ctcgctgtac atgcagcagg
     2161 tgctcggcta cagcgcgatc aaggccggac tctcctacct gccgctggcg gtctcgatca
     2221 tcctctcggc cgggatcgcc tcgcagctgg tgaccaaagt cggcttcaag ccgatcctcg
     2281 cgaccggcat ggtgctgatc gccgccggcc tcgtctggtt ctcgcagatc tccgccgacg
     2341 gcagcttcct cagcgacatc ctcgggcctt cgctgctggc agccgtcggg ctcggcttct
     2401 ccttcgtgcc ggtgacgatc gccgccgtct ccggcatccc cgaccacgag cagggcctcg
     2461 ccagcggcct gatcaacacc tcccagcagg tcggcggcgc gctcggcctg gcgatcctcg
     2521 ccgccgtctc cagctcggtg accggcaact cgatcgaccc cgaggtgctc accgaaggct
     2581 tccaggacgc cttcctggtc ggcgccgcct tcgccgtcct cggcctgatc gccaccctct
     2641 tcctgatccg caaccgcgac agcaaagctc acgtggcgct gagccaagag gtcgctgtcg
     2701 agccggagta gcgcgtcttc ctcgcgcctt tcggggctgg aggactcggc ttcaaccgaa
     2761 catcgtttgg taagccgccg ggcgggcggt tagggtagtc gcgtgtcgtt cgcccacccc
     2821 tcgctagctc gtgctctcgc tggactgctc gcaattgcgg cgctcggctg cgcggtgctg
     2881 ctgttcagcg accgcggcga tgccgcggtg accggggtca cggcggtcga ctcttccgcc
     2941 tgcggtccgg tccagtacgg cggcagcggc gcggctgacg cgctgatcgt ctccgacctg
     3001 ccgctgcagg gggactcgcg acggcgctcg ctgcagatga acgaagcgat ccagatcgtg
     3061 ctcgaagacg cgggctggaa agccggcggg cgcaacgtcg cctttcaggc ctgcgacgac
     3121 tcgctcgccg acaccgggct ctggagcaag gtgcagtgca aggcgaacgc caaggcctac
     3181 gccgccaacc cgagcgtgct cgcggtgatc gggacctaca actcgggctg cgccgaaaac
     3241 atgatcccga tcctcgggca ggcacccggt ggagggctcg cgatggtctc ccccggaaac
     3301 accctgatct gcctcaccca gagcagcccg acctgcggca acggcacgcc gaagagcctc
     3361 taccccgctc gccgcaacta cgcccgcgtc gtccccaacg acgcctacca gggcgccggc
     3421 ctcgccaagt tcgcgcaggg caagggcatc cgcagcgcct tcatcctcta cgcggccggc
     3481 gacgtcacca gcttcggcca ggcgaagacc ttcaacggcg ccgccaagaa gctcgggatc
     3541 aagatcaccg gcttccgcca gtggaacccg aaggcctacg aatacaagcg gctgatgaac
     3601 gtggtcgggg cgacggcacc cgacgcggtc ctgctcgccg gcctgaccga acagaacggc
     3661 gcccagctga tcaaggacaa ggtcgcggcg ctcgggccga acgacggtca cgtcaagctg
     3721 ctcgcgccgg atggtttcgc gcagcaggcc acgatcgacg acgccgggtc ggcgtccgcc
     3781 ggcatgttcg tgagcctgcc gggcagagtg cccgacagcc ttaccggcga gggtgccgcg
     3841 ttcgtcggcg a
//
LOCUS       DASRIG010000291         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2476473, whole genome shotgun sequence.
ACCESSION   DASRIG010000291 DASRIG010000000
VERSION     DASRIG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2476473"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFB52_07945"
     CDS             complement(<1..1146)
                     /locus_tag="VFB52_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06304.1"
                     /translation="MSSLIANSNSRKWWTLGAVCVATFMLLLDITVVNTALPDIQKDL
                     GGSFSDLQWVVDAYALSLAALVLTAGSLADRLGRRRIFAVGLIVFSIASLLCALATDP
                     TFLNLARGLQGVGGAIMFAVSLALVAQEFPSGAERGMAMGIYGATIGIAVAIGPLLGG
                     LLTDGFGWESVFLINVPIGLVTVAVTYWKLVESRDPNATRIDWGGLVTFSAALFALVL
                     ALVRGNAEGWGSTPIVSLFAAAAVLFVAFIAIETRVKQPMLPLGLFKRPAFTGVQLGA
                     FAVSGSMFAIFLYLTLYLQGFLGHSPVEAGLRYLPITLASFFVAPFAGMALAKVQARY
                     LLSGGLALVGAGLLMMGSLGVNSEWTALLVGFIVGGGGIGLVNPVIAD"
     gene            1234..1737
                     /locus_tag="VFB52_07950"
     CDS             1234..1737
                     /locus_tag="VFB52_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZO06305.1"
                     /translation="MGAPPGTPLNQIPLTGLFEIAIDAMLAEFRVDLAEAGYDDLRPT
                     HGCVFRFVHDDGLRLTELAALAGLTKQSVGEIVDELVALGYVERVPDPADKRAKLICL
                     TPKGEQAQLTAFGLFARIQDRWAKRYGADRIATLRSLLEEIALTELPDAVPELSRPAP
                     SELAAAT"
     gene            1795..1868
                     /locus_tag="VFB52_07955"
     tRNA            1795..1868
                     /locus_tag="VFB52_07955"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1829..1831,aa:Asp,seq:gtc)
     gene            2088..>2637
                     /locus_tag="VFB52_07960"
     CDS             2088..>2637
                     /locus_tag="VFB52_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06306.1"
                     /translation="MDMGVEMKQREKTLQNIARLRRAEKETPSADISAVREDLESQLG
                     GTVSRSLSASLLGVSHTALNNWIAAGDVPVVISERGRKEVPIPTLLDLQERVDLERKS
                     GRRKLHTLEPVMTEARQRAERMRPRVSVSGSHVRTDPHRAPELRSLAYHRAIAPRLRR
                     PTIDAARRKLRRWKEDGRIDPRY"
BASE COUNT          508 a          881 c          872 g          376 t
ORIGIN      
        1 gtcggcgatc accgggttga ccaggccgat cccgcccccg ccgacgatga agccgaccag
       61 cagcgcggtc cactccgagt tgaccccgag gctgcccatc atcagcagcc cggcgccgac
      121 cagcgccagc ccgccgctga gcaggtagcg agcctgcact ttcgccagcg ccatcccggc
      181 gaagggggcg acgaagaagg aggcgagcgt gatcggcagg tagcgcagcc cggcttcgac
      241 cggcgagtga ccgaggaagc cctgcaggta gagcgtcagg tagaggaaga tcgcgaacat
      301 cgagcccgag accgcgaagg cgccgagctg gacgccggtg aaggcgggcc gcttgaacag
      361 gccgagcggc agcatcggct gcttgacccg ggtctcgatc gcgatgaagg cgacgaagag
      421 cacggcggcg gcggcgaaca gcgagacgat cggggtgctg ccccagccct cggcgttgcc
      481 gcgcaccagc gccagcacca gggcgaacag cgcggccgag aaggtgacca ggccgcccca
      541 gtcgatccgg gtcgcgttcg ggtcgcgcga ctcgaccagc ttccagtagg tgaccgcgac
      601 cgtgaccagc ccgatcggca cgttgatcag gaagaccgac tcccagccga agccgtcggt
      661 gagcaggccg ccgagcagcg gcccgatcgc gaccgcgatc ccgatcgtcg ccccgtagat
      721 gcccatcgcc atgccgcgct cggcgccgct ggggaactcc tgcgcgacca gcgccaggga
      781 gacggcgaac atgatcgcgc cgccgacgcc ctgcaggccg cgggcgaggt tgaggaaggt
      841 cgggtcggtg gcgagcgcgc agagcagcga ggcgatcgag aagacgatca ggccgacggc
      901 gaagatgcgg cggcggccga gccggtcggc gagcgagccg gcggtcagga ccagcgccgc
      961 gagcgagagc gcgtaggcat cgaccaccca ctgcaggtcg gagaagctgc caccgaggtc
     1021 cttctggatg tccggcagcg ccgtgttgac gacggtgatg tcgagcagga gcatgaaggt
     1081 ggcgacgcag acggcgccga gcgtccacca cttgcggctg ttcgagttgg caatcaggga
     1141 agacataaag gggctcctcg gcatcgagta caattcgtaa ggaagccttg tcaggctaac
     1201 tgatcaatcg accttatcag gtaaactgac gatatgggcg cgcccccagg aactcctctc
     1261 aaccagattc ctctcaccgg cctcttcgag atcgcgatcg acgcgatgct ggctgagttc
     1321 cgcgtcgacc tcgccgaggc tggctacgac gacctccgcc cgacccacgg ctgcgtcttc
     1381 cgcttcgtcc acgacgacgg cctccggctc accgagctcg ccgctctggc gggcctgacc
     1441 aagcagtcgg tcggcgagat cgtcgacgag ctcgtcgccc tcggctacgt cgaacgcgtc
     1501 cccgaccccg ccgacaaacg cgccaagctg atctgcctca cccccaaggg cgaacaggcc
     1561 cagctcaccg ccttcggtct cttcgcccgg atccaggacc gctgggccaa gcgctacggc
     1621 gccgaccgca tcgccaccct ccgctccctg ctcgaagaga tcgctctcac cgagctcccc
     1681 gacgccgtgc ccgagctttc ccggcccgcg ccctccgagc tagccgccgc gacctagtac
     1741 ccgctagatt tcccctccgg gccgcaccca gctggcccat ccgaggcacc cctcgggggc
     1801 gtagctcagt tggttagagc gccggcctgt cacgccggag gtcgcgggtt cgagtcccgt
     1861 cgctcccgct tcggcgtgcc gcatttgatc tcagatcggt ttccgcggga cgccgatccg
     1921 gtgtccagcg acaagacgac gggtgagttg tgcgtgcggt aatcacaaac cgccggatca
     1981 tggaaacgca gccatgaaag ttgcaggaat acagaggtag ttgcaagttt aaggatctag
     2041 gtaaactttc aataactgga tgaaatacgc aactttagag ataagatgtg gatatgggcg
     2101 tggaaatgaa acagcgcgag aaaacgctgc agaacattgc gcggttgcga cgggctgaga
     2161 aagaaactcc cagcgcggac atttcagcgg ttcgtgaaga cctcgaatcg caactgggcg
     2221 gaactgtctc gcgcagcttg tccgcaagtc tgctgggagt gagccacacc gcactcaaca
     2281 actggatcgc ggccggtgac gttccggtgg tcatctcgga gcggggacgg aaggaggttc
     2341 caatcccaac cctgctggat ctccaggaga gggtcgactt ggagcgcaag tctgggcggc
     2401 ggaagctcca cacgctggag ccggtcatga ccgaggcgcg ccagcgcgct gagcggatgc
     2461 gcccgcgggt ctcggtctcc ggatcccatg ttcgaaccga cccgcatcgc gcccctgagc
     2521 tgcgcagcct tgcctatcac cgagcgatcg ctccgcgact gcgccgcccg acgatcgacg
     2581 ccgcacggcg caagctgagg cggtggaagg aagacggaag gatcgatcct cgctacg
//
LOCUS       DASRIG010000292         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1199980, whole genome shotgun sequence.
ACCESSION   DASRIG010000292 DASRIG010000000
VERSION     DASRIG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199980"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFB52_07965"
     CDS             complement(<1..787)
                     /locus_tag="VFB52_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZO06307.1"
                     /translation="MASRGERPLIGVTTSEVRPAERVTPLAEGEPRGREMALGMPYMK
                     SVEAAGGLPVVIPPLPEEAIEPLLDRLDGICLSGGPDIDPATYDAEPHPELGPIEPDL
                     DRFELDVARRADAREMPILAICRGTQAINIVRGGILHQHIPELSAEIPHRQKVASDQP
                     SHAVEIRPESHLAAVIEGQEIEVNSFHHQAIDRLGEGLEVSACAPDGTIEAIEDPGRR
                     FLIGVQWHAETLTHRPYEAALFERFVDACREASAEFARRSGREV"
     gene            complement(799..2247)
                     /locus_tag="VFB52_07970"
     CDS             complement(799..2247)
                     /locus_tag="VFB52_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZO06308.1"
                     /translation="MATTLNPARPFPGAGKYPLDTGHFDEAFVPTGAPRPPYAELIDA
                     LARQDLVVLRERVRSTVASLGLNFGPGKPMVVDPVPRILAGAEWTALQPGLLQRARAL
                     NAFVADVYGGQRIFDAGLVPRGLLETSSGYEPKMHGLLDPSVPAATVAGLDLIRDEEG
                     NLLVLEDNLRMPSGATYAIAVREAVAPEIETATKPLPLDDYVATLGEAIRAAAPAGRE
                     EANAALLSDGPESGAWYEHKRLSRELGIAIVQPRQLETERGRLYARSSARKRTQVDVL
                     YRRTDEDRLSTPEGEPTALGAVLLPALESGRLRCVNAFGTGVADDKLAHAYAEKMIRF
                     YLDEEPLLRSVPSFDLSAEEDREAAMARLDELVLKPRDGFGGQGVTIMPRATAAQRRR
                     AIGLVRRRPEGFVAQETIQLSSHPTVCGGRLRPRRVDFRPYVVSGPRGASTMVGGLTR
                     YARGAGEMVVNSSRGGGCKDTWVVASPTGGSR"
     gene            complement(2345..>3508)
                     /locus_tag="VFB52_07975"
     CDS             complement(2345..>3508)
                     /locus_tag="VFB52_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZO06309.1"
                     /translation="ASTPPPRLTPMALIVLEETLRPDAAETIEFMRHEEVDLKLISGD
                     ARATVTAVAYGVGVPREAGVIEGPDLPEDPEGLERAALENTVFCRIKPEQKKALVAAL
                     VGAGRYVAMIGDGVNDVPALKQARMAVAMGSGTQVTKGVADVVLLKDQFSRLPEAVGE
                     GRRIARNIHRLGRLYLTKTVYAATLILLVAVPGFAFPFLPRHLTVAALLTIGIPSFVL
                     ALMPSDGPLYRGRLLRALAAFAVPAGVAIALASIISFFLVDTIAGASLEAGRTAATTT
                     LIVLGLAFLLLLERGPGREDIAIQSNMLAMVAGLGALYAGILAIAPARDFFGLELLNA
                     TQWFLSMLAAAIGLIIASVLWRLPQVVALEAPVSSEDEPPSPMEEAEAPTRVL"
BASE COUNT          498 a         1229 c         1294 g          487 t
ORIGIN      
        1 cgacctcgcg tccgcttcgt cgggcgaact cggcgctggc ctcgcggcag gcatcgacga
       61 agcgctcgaa cagggccgcc tcgtaggggc ggtgggtgag cgtctcggcg tgccactgga
      121 ccccgatcag gaagcggcgg ccggggtcct cgatcgcctc gatcgtgccg tcgggagcgc
      181 acgcgctgac ctcgaggccc tcgccgagcc ggtcgatcgc ctggtggtgg aaggagttca
      241 cctcaatctc ctgaccctcg atcaccgccg caaggtgact ctcgggtctg atctcaaccg
      301 cgtggctggg ctggtcgctg gcgaccttct ggcggtgcgg gatctccgcg gagagctccg
      361 ggatgtgctg gtggaggatc ccgccgcgga cgatgttgat cgcctgggtg ccgcggcaga
      421 tcgccaggat cggcatctcg cgggcgtcgg cgcgccgcgc cacgtccagc tcgaagcggt
      481 cgaggtcagg ctcgatcggg ccgagctcgg ggtgcggctc ggcgtcgtag gtcgccgggt
      541 cgatgtcggg accgccggag aggcagatcc cgtcgagccg gtcgagcagc ggctcgatcg
      601 cctcctcggg cagcggcggg atcaccaccg gcagcccccc cgccgcctcg acgctcttca
      661 tgtagggcat gccgagcgcc atctcgcgcc cgcgcggctc accctcggcc aacggcgtga
      721 cccgctcagc cgggcgcacc tcggaggtcg tgacgccgat cagcggtcgc tcgccgcggg
      781 aagccaatga acgccgcctc atcgggagcc gccggttggg cttgcaacca cccaggtgtc
      841 cttgcaacca cccccgcgcg agctgttgac gaccatctct ccggccccgc gggcgtagcg
      901 ggtcagcccg ccgaccatcg tcgacgcccc ccgcggcccg ctgaccacgt agggacggaa
      961 gtcgacccgg cgtgggcgca ggcggccgcc gcagacggtc gggtggctgg agagctggat
     1021 cgtctcctgg gcgacaaagc cctccggacg ccgccgcacc agcccgatcg cgcgtcggcg
     1081 ctgcgccgcg gtggcccgcg gcatgatcgt taccccctgg ccgccgaaac cgtcgcgcgg
     1141 cttcagcacc agctcgtcga gccgcgccat cgccgcctcg cggtcctcct cggcggagag
     1201 gtcgaagctc ggcaccgagc gcagcagcgg ctcctcgtcg aggtagaagc ggatcatctt
     1261 ctcagcgtaa gcgtgggcga gcttgtcgtc ggcgacgccg gtgccgaagg cgttgacgca
     1321 gcgcagccgg ccggattcga gcgccggcag caggaccgcg ccgagcgcgg tcggctcgcc
     1381 ctcgggcgtg ctcagccggt cctcgtcggt gcgccggtag agcacgtcga cctgggtccg
     1441 tttgcgggcc gagctgcggg cgtagaggcg gccgcgctcg gtttcgagct ggcgcggctg
     1501 cacgatcgcg atcccgagct cgcggctgag ccgcttgtgc tcgtaccagg caccgctctc
     1561 ggggccgtcg gagagcagcg ccgcgttcgc ttcctcgcgc cccgcggggg cggcggcgcg
     1621 gatcgcctcg ccgagcgtcg cgacgtagtc gtcgagcggc agcggcttgg tcgcggtctc
     1681 gatctcgggg gcgaccgctt cgcgcacggc gatcgcgtag gtcgcacccg agggcatgcg
     1741 cagattgtcc tccagaacca gcaagtttcc ctcctcgtcg cggatcagat ccagccccgc
     1801 gacggtagcc gcagggacgg agggatcgag caggccgtgc atcttcggct cgtaccccga
     1861 ggaggtctcg agcaggccgc gcgggaccag cccggcgtcg aagatccgct ggccgccgta
     1921 gacgtcggcg acgaacgcgt tgagcgcccg ggcccgttgc agcaggccgg gttgcagcgc
     1981 cgtccactcg gcgccggcca ggatccgcgg caccgggtcg acgaccatcg gcttgccggg
     2041 cccgaagttg agccccagcg atgccacagt tgaccggacg cgctcgcgca gcaccaccag
     2101 gtcctgccgc gcgagcgcgt caatcagctc cgcgtaggga gggcgcggag ccccggtcgg
     2161 gacgaacgcc tcgtcgaagt gccccgtgtc gagcgggtac tttccggcac cgggaaaggg
     2221 acgggccgga ttcaacgtcg tcgccatggc agcacgctac ggctggtacc cgcgattcga
     2281 catggcccaa cgtcgaaaaa cgcgctgccg cgactagaca gaggtctaag agcggtggag
     2341 gccgtcagag caccctggtc ggcgcctcgg cctcctccat cggcgacggc ggctcgtctt
     2401 cgctgctgac cggcgcttcc agggccacga cctggggcag gcgccagagc acgctggcga
     2461 tgatcaggcc gatcgcggcg gcgagcatcg agaggaacca ctgggtcgcg ttcagcagct
     2521 ccagcccgaa gaagtcgcgg gcgggggcga tcgcgaggat cccggcatag agggcgccga
     2581 ggccggcgac catcgccagc atgttgctct ggatcgcgat gtcttcgcgg ccggggccgc
     2641 gctcgagcag gagcaggaag gcgaggccaa ggacgatcag cgtcgtcgtc gccgcggtgc
     2701 ggccggcttc gaggctggcg ccggcgatcg tgtcgacgag gaagaaggag atgatcgagg
     2761 caagcgcgat cgccaccccg gcggggaccg cgaaggcggc gagggcgcgc agcaggcggc
     2821 cgcggtagag cggcccgtcg gagggcatca gggcgagcac gaaggagggg atgccgatcg
     2881 tcagcaacgc cgccacggtc aggtggcggg ggaggaaggg gaaggcgaag ccgggcacgg
     2941 cgacgagcag gatcagggtc gcggcgtaga cggtcttggt caggtagaga cggccgagcc
     3001 ggtggatgtt gcgggcgatc cggcggccct cgccgaccgc ctcgggcagg cgcgagaact
     3061 ggtctttgag caggaccacg tcggcgacgc ctttggtcac ctgggtgccc gagcccatcg
     3121 ctaccgccat ccgcgcctgc ttcagcgccg gcacgtcgtt gaccccgtcg ccgatcatcg
     3181 ccacgtagcg gccggcgccg accagcgccg cgaccagcgc tttcttctgc tccggcttga
     3241 tccggcagaa gacggtgttc tccagcgccg cccgttccag cccctccggg tcctcgggca
     3301 ggtcgggacc ctcgatcacg ccggcctcgc gcggcacccc gaccccgtag gcgaccgcgg
     3361 tcacggtcgc ccgcgcgtcg ccggagatca gcttcaggtc gacttcctcg tggcgcatga
     3421 actcgatcgt ctcggccgcg tccgggcgca gcgtctcctc gagcacgatc agcgccatcg
     3481 gggtcagtcg cggcggcggg gtgctggc
//
LOCUS       DASRIG010000293         4788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_395327, whole genome shotgun sequence.
ACCESSION   DASRIG010000293 DASRIG010000000
VERSION     DASRIG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395327"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFB52_07980"
     CDS             <1..740
                     /locus_tag="VFB52_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO06310.1"
                     /translation="DAATYQRISVPHEEWAEAVLERLPLRGEETVLDAGCGTGRVTRM
                     LVERLPDGRVIAVDGSAAMVEKVREVLRPQDEAFVADLSALELEQPVDAIVSSAVFHW
                     IKDHDELFRRMRACLKPGGRLAAQCGGAGNIDGFRRAGKTVAEREPYAPHFEDFGDLW
                     FYAGAEETEARLRAAGFEQIRCWLEPWQVSPPEPVDFTRTIMLSGHIDRLPAELHDRF
                     VADVIAAAGEPLVLDYVRLNIEAVAAA"
     gene            761..1831
                     /gene="leuB"
                     /locus_tag="VFB52_07985"
     CDS             761..1831
                     /gene="leuB"
                     /locus_tag="VFB52_07985"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZO06311.1"
                     /translation="MSDAPRIVVLAGDGIGPEIVDATTKILDSLGEFTYEPQLLGGCS
                     IDEHGTALTDEVLEACKSADAVLLGAVGGPKWDTTNPDDPRPEQGLLGLRKGMGLYAN
                     LRPVRPSPALVDASPLRQDVIAGTDLLVVRELTGGIYFGASGREGDRAYDTCEYTAAE
                     IERIARTAFEAARRRAEGSGRKPKVTSVDKANVLESSRLWREVVIRVAPDYDDVTLDH
                     LLVDNAAMQLVSRPADFDVIVAENLFGDILSDEAAMLTGSLGMLPSASLGADGDPGLF
                     EPVHGSAPDIAGQGVANPLATFLSAALMLRHGLERPDEAARIEAAVDAVLARGLRTAD
                     LVPSGTSSVGTAEITSAVLDEL"
     gene            complement(1832..2617)
                     /locus_tag="VFB52_07990"
     CDS             complement(1832..2617)
                     /locus_tag="VFB52_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06312.1"
                     /translation="MKAKLRLPKLTYSNVIASLALFVALSGVAVAAGLPKNSVGPNQI
                     KRGAVNTVKLKNRAVTSAKLANKAVTAGKLGPNSVTPGNIGNGAITSAKIGNSAVIAA
                     SIKNGVVTTNKLANEAVNSAKLGKGSVTLAKLASDVAPLLGTLKSGQTLRGIFYIVGN
                     TSENELLGDSISYQFPISGNPPAVNVLKKGATNANCSGIGSGSAPAATAGNLCVYLTE
                     EENLADTEGLTAQNNQRLGFGLAAKAKANGKVIAAGQWAVTAP"
     gene            2707..3657
                     /locus_tag="VFB52_07995"
     CDS             2707..3657
                     /locus_tag="VFB52_07995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZO06313.1"
                     /translation="MEPQDLIWKNGEFVAWEDAQVHVLTHGLHYGTGVFEGIRCYETE
                     RGPAVFRHADHLRRLEQSAELYYLQMPYSAEEIRQATHGLLSRCGLGSAYIRPIAFRG
                     YGSLGLFAKDAPVEVVIAAFPWGAYLGEESAGRGIRAKVSSWRRISPASTIPHAKASG
                     SYLNSILAKTESHNSGYDEAILLDERGFVCEGSGENIFLVRDGEIVTPGHVASILDGI
                     NRKSVIQIARDLGYTVVERDVARSELYLAEEVFMTGTAAEMVPVREIDDHEIGEPGEI
                     TRHIQAKFHDALYGRAEEYLEWLDFVEAPAQVDAPDKVGS"
     gene            3777..>4788
                     /locus_tag="VFB52_08000"
     CDS             3777..>4788
                     /locus_tag="VFB52_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZO06314.1"
                     /translation="MEQVNLYDTTLRDGMQGEGMSLSAAEKARVVLALDKLGVQMIEA
                     GFPSSNPKEVELFEQLAELPLEQATVCAFGMTRRRDTAVEDDPALATLVGAFTPVVTM
                     VGKTWSLHLEKVTKVSREENLAMIAESVAYCVAQGKRVVYDAEHFFDAYRDDTGYALS
                     CLEAAVGAGAENVTLCDTNGSSLPDQIAAATAVVRQRLGEGTEIGIHVHDDAGVGVAN
                     SLAAVREGARQVQGTINGYGERCGNANLTSILPALQLKMGFDVVSAEQLASLTETAHF
                     IDELCNVTPNPDAPYVGRNAFAHKGGMHVAGIEADARTFEHLDPETVGNQRAVLPSEL
                     SGK"
BASE COUNT          719 a         1687 c         1607 g          775 t
ORIGIN      
        1 gggacgccgc cacctaccag cggatctcgg tcccgcacga ggagtgggcc gaggcggtcc
       61 tggagcggct gccgctgcgc ggtgaggaga ccgtgctcga cgccggctgc ggcaccggcc
      121 gggtcacccg gatgctggtc gagcgcctgc cggacggccg cgtgatcgcc gtcgatggct
      181 ccgccgcgat ggtcgagaag gtgcgcgagg ttctgcgccc gcaggacgag gccttcgtcg
      241 ccgacctctc cgctctggag ctcgagcagc cggtcgacgc gatcgtctcc agcgccgtct
      301 ttcactggat caaggaccac gacgagctct tccgccgcat gcgcgcctgc ctcaagccag
      361 gcggtcgcct cgccgcccag tgcggcggcg ccgggaacat cgacggcttc cgccgcgccg
      421 gcaagacggt cgccgagcgc gagccctacg cgcctcactt cgaggacttc ggcgacctct
      481 ggttctacgc cggcgccgag gagaccgaag cgcgcctgcg cgccgccggc ttcgagcaga
      541 tccgctgctg gctggagccc tggcaggtgt cgccgcccga gccggtcgac ttcacccgca
      601 cgatcatgct ttccggccac atcgaccgcc tgcccgccga gctgcacgac cgcttcgtcg
      661 ccgacgtgat cgccgcggcc ggcgagccgc tggtgctcga ctacgtgcgg cttaacatcg
      721 aggccgtcgc ggccgcctaa cgcgtagagt cccgcgccga atgagcgacg ccccccgcat
      781 agtcgtcctt gccggcgacg ggatcggtcc cgagatcgtc gacgcgacca ccaagatcct
      841 cgattcgctc ggcgagttca cctacgagcc gcagctgttg ggcggctgct cgatcgacga
      901 gcacggcacc gcgctcaccg acgaggtcct ggaggcctgc aagtccgccg acgcggtcct
      961 gctcggcgcc gtcggcggtc ccaaatggga caccaccaac cccgacgacc cgcgccccga
     1021 gcaggggctg cttggcctgc gcaagggaat gggcctctac gccaacctgc gcccggtgcg
     1081 cccgagcccg gccctggtcg acgccagccc gctgcgtcag gacgtgatcg ccgggaccga
     1141 cctgctggtc gtccgcgagc tcaccggcgg catctacttc ggcgcctccg gccgcgaggg
     1201 cgaccgcgcc tacgacacct gcgagtacac cgctgcggaa atcgagcgga tcgcccgcac
     1261 cgccttcgag gcggcgcgcc ggcgcgccga gggctccggc cgcaaaccga aggtcacctc
     1321 ggtcgacaag gccaacgtgc tcgagagctc gcggctctgg cgcgaggtgg tcatccgcgt
     1381 cgcccctgac tacgacgacg tcacgctcga tcatctgctg gtcgacaacg cggcgatgca
     1441 gctcgtctcc cggccggccg acttcgacgt gattgtcgcc gagaacctgt tcggcgacat
     1501 cctcagcgac gaggcggcga tgctgaccgg ctcgctgggc atgctgccct ccgccagcct
     1561 cggtgccgac ggcgacccgg gcctgttcga gccggtccac ggctcggccc ccgacatcgc
     1621 cggccagggc gtcgccaacc cgctcgcgac cttcctctcg gcggccctga tgctgcgcca
     1681 cggcctcgag cgccccgacg aggcggcgcg gatcgaggcc gccgtcgacg cggtcctcgc
     1741 gcgaggattg cgtacggccg acctcgttcc gagcggaacg tcgtccgtgg ggacggcgga
     1801 gatcacctcc gccgtcctgg acgagctcta gttacggggc ggttaccgcc cactggccag
     1861 cggcgatgac tttgccgttg gctttcgctt ttgccgccag gccgaagccg agccgctggt
     1921 tgttctgcgc ggtcaggcct tcggtgtcgg cgaggttttc ttcctcggtc aggtagacgc
     1981 agaggttgcc ggcggtcgcc gccggggccg agccgctgcc gatcccggag cagttcgcgt
     2041 tggtcgcgcc ttttttcagc acgttgaccg cgggcgggtt gccgctgatc gggaactggt
     2101 agctgatcga gtcgccgagc agctcgtttt cggacgtgtt gccgacgatg tagaagatcc
     2161 cgcgcagcgt ctggccggac ttcagcgtgc ccagcagcgg cgccacgtcg gaggccagct
     2221 tcgccagcgt caccgagcct ttgccgagct tggcgctgtt cacggcttcg ttggccagct
     2281 tgttggtggt gaccacgccg tttttgatcg aggcggcgat cacggcgctg ttgccgatct
     2341 tggcgctggt gatcgcgccg ttgccgatgt tccccggcgt caccgagttg ggcccgagct
     2401 tgcccgcggt gaccgctttg ttggccagct tggcgctggt cacggcccgg ttcttgagtt
     2461 tgaccgtgtt gacggcgccg cgcttgatct ggttggggcc gacgctgttc ttcggtagcc
     2521 ccgcggctac tgcaacgccg ctgagcgcga cgaacagggc caagctcgcg atcacgttcg
     2581 agtaggtgag cttcggcagt cgaagcttcg ctttcatctg gaatcgctcc ttttgtggtg
     2641 gttctgagcg gtcattcgag cccgtggggg tgaagacctt cgatccatcg ggcatacttc
     2701 tccaacatgg agccgcagga tttgatttgg aagaacggcg agtttgtcgc ctgggaggac
     2761 gcccaggtgc acgtgctcac gcacggtttg cactacggaa ccggtgtatt cgagggaatt
     2821 cgctgctacg aaaccgagcg cggcccggcc gtcttccgcc acgccgacca cctgcgccgc
     2881 ctcgagcagt cggccgagct ctattacctg caaatgccgt actcggcgga ggaaatccgc
     2941 caggccacgc acgggctgct gagccgctgc gggctcggtt ccgcctacat ccggccgatc
     3001 gccttccgcg gttacggcag cctcgggctg ttcgccaagg acgccccggt cgaagttgtg
     3061 atcgctgctt tcccctgggg cgcctacctt ggcgaggaga gcgccgggcg cgggattcgc
     3121 gccaaggtct cgagctggcg ccggatcagc ccggcgagca cgatcccgca cgccaaggct
     3181 tcgggctcct acctcaactc gatcctcgcc aagaccgagt cgcacaactc tggctacgac
     3241 gaggcgatcc tgctcgacga gcgcggcttc gtctgcgagg gctcgggcga gaacatcttc
     3301 ctggtccgcg acggcgagat cgtcaccccg gggcacgtcg cctcgatcct cgacgggatc
     3361 aaccgcaaat cggtgatcca gatcgcccgt gacctcggct acaccgtcgt cgagcgcgat
     3421 gtcgcccgct ccgagctcta cctcgccgag gaggtcttca tgaccgggac cgcggccgag
     3481 atggtgccgg tgcgcgagat cgacgaccac gagatcggcg aacccggcga gatcacccgc
     3541 cacatccagg cgaaattcca cgatgcgctc tacgggcgcg ccgaggagta cctcgagtgg
     3601 ctcgacttcg tcgaggcgcc ggcccaggta gacgcacccg ataaggtcgg gtcatgagtt
     3661 ccctgctttt catcgagatc gggcgaatta ccgcagccgc ctagcggctg gcgcggcgta
     3721 ttcgttgcgc accgggcttc ggcccgcccc gatcttcaga ctgaaaggca gtaacgatgg
     3781 aacaggtgaa cctttacgac acgactctgc gtgacggcat gcagggtgag ggaatgtcgc
     3841 tctcggcggc cgagaaggcg cgcgtggtgc tggcgctaga caagctcggc gtgcagatga
     3901 tcgaggccgg cttcccgagc tcgaacccga aggaagtcga gctgttcgaa cagctcgccg
     3961 agctgccgct cgagcaggcg accgtctgcg ccttcgggat gacccggcgc cgcgacaccg
     4021 cggtcgagga cgacccggcg ctggcgaccc tggtcggcgc cttcaccccg gtggtgacga
     4081 tggtcggcaa gacctggagc ctgcacctgg agaaggtgac caaggtctca cgcgaggaga
     4141 acctggcgat gatcgccgag tcggtcgcct actgcgtcgc ccagggcaag cgggtcgtct
     4201 acgacgccga gcacttcttc gacgcctacc gcgacgacac cggctacgcg ctcagctgcc
     4261 tggaggcggc ggtcggggcc ggtgccgaga acgtcaccct ctgcgacacc aacggctcca
     4321 gcctgcccga ccagatcgcc gccgcgaccg cggtggtgcg acagcggctg ggggagggga
     4381 ccgaaatcgg catccacgtc cacgacgacg ccggcgtcgg cgtcgccaac tcgctcgccg
     4441 cggtgcgcga gggcgcccgc caggtgcagg gcacgatcaa cggctacggc gagcgctgcg
     4501 gcaacgccaa cctgacctcg atcctgccgg cgctgcagct gaagatgggc ttcgacgtgg
     4561 tctccgcgga gcagctcgcc tcgctgaccg agaccgcgca cttcatcgac gaactctgca
     4621 acgtcacccc gaaccccgac gcgccctacg tcggccgcaa cgccttcgcc cacaagggcg
     4681 gcatgcacgt cgccgggatc gaggccgacg cgcgcacctt cgagcacctc gatccggaga
     4741 cggtcggcaa ccagcgcgcc gtgctgccct ccgagctctc gggcaaag
//
LOCUS       DASRIG010000294         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_149162, whole genome shotgun sequence.
ACCESSION   DASRIG010000294 DASRIG010000000
VERSION     DASRIG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149162"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VFB52_08005"
     CDS             complement(<1..1445)
                     /locus_tag="VFB52_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZO06315.1"
                     /translation="MSERDYLAAVRERVVVFDGGMGATLEMFDLTQEDYGGLLGKCHE
                     ALVLNRPDVIEGVHTSMLEAGAEVVETDTFQGSRLKLEEWGLGEQTMEINTKAAEIAR
                     KAAGPDRFVAGSIGPTGFLPASTDPTLGDISFAKLAEVFAEQATGLVEGGADLIIIET
                     AQDILEVKAAIFGAREAFTQTGRTLPLQISVSLLPNGGKMLLGTDIQSVLTTLTALDV
                     DVVGLNCSTGPEDMRDAIRFLGETSPLPVHCIPNAGLPLQGPDGETIFPEEPEPLAAT
                     LGEFVERYGVGIVGGCCGTTPAHIAAIRARVDGKVPAERPAPGQIEVSSMMTSTPLVQ
                     EPRPTLVGERVNSQGSKKAKELLLADDYDGLVQVAEDQVEGGAHVLDVCVALTERQDE
                     DEQMSAVVKRVSLTQPAPIQVDSTEPEVIQAALETIPGRAIVNSINLEAGRDKADTVI
                     PLAKAHGAALIALTIDEVGMAKTAERKLEIA"
     gene            complement(1459..2193)
                     /locus_tag="VFB52_08010"
     CDS             complement(1459..2193)
                     /locus_tag="VFB52_08010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06316.1"
                     /translation="MECGIAEAWFGPVQVTTFEALPDDPGMNTILGAAEPGAVEEGHL
                     ANAIAWADEFDVGYRVSVARDRPGTAAAENYLNHQGFEQGRGAWKYVRDVSWPDLPGN
                     PAITVWEINGEDAAGETMVFDAAPALGFPSKAASLLFGLPNQERWRTYTAELEERIVS
                     FGSMLIDGDVAQVGLAATLPEARGFGCNLVLLRRRILDAIEAGCNTIFAELGESEAEG
                     IAVAGRNLLRAGFVPAYRSMNWLRPR"
     gene            complement(2451..4025)
                     /locus_tag="VFB52_08015"
     CDS             complement(2451..4025)
                     /locus_tag="VFB52_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO06317.1"
                     /translation="MESGTAEKIIGTNGSASTGSGAEPKQIEVLNPANGQLIQTVPVT
                     SPEEVAATVARVRANQADWEAMGIEGRYHWLGKLRDWILDNQERILDTMQRETGKVRA
                     DASNEPIYLADIINFYGSRAAKFIGEEAVRPHSALLSSKKLRVQYRPHPVVGIISPWN
                     FPLILSIGDAVPALQAGAAVVIKPSEFTPLGLAEIVDAWKHEIGGPDVFDVVQGVGET
                     GGALVDNVDFIQFTGSDRTGRKVMARAAETLTPVSLELGGKDPMIVLADADLDRAANA
                     AAWGGMMNSGQICMSVERIYVEEPAYDEFVAKLTAEVGKLRQGPDASGHDADVGAMTS
                     PNQIGIVEDHVDDALAAGARALTGGKRVESPGDYFEPTVLVDVDHSMKVMRDETFGPV
                     VGVMKVRDAEEALKLANDSRYGLSGSVFGEKQRAEQVARRVECGAVNVNDVLVNYFAS
                     DVPMGGWKESGIGYRHGAPGIKKFCRTESVVITRFAGKREPTWYPYTRARRGLVGRIA
                     VAFNARDLKRRLGRKG"
     gene            complement(4147..>5240)
                     /locus_tag="VFB52_08020"
     CDS             complement(4147..>5240)
                     /locus_tag="VFB52_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06318.1"
                     /translation="EKQGGSDVRANVTRAVEIGDGEYEIHGHKWFCSAPMCDAFLVLA
                     QAEDGLSCFVLPRFRPDGTRNAFHLQRLKDKLGNRSNASSEIEFDNASAWLVGDEGAG
                     VRTIIEMVNHTRLDCVLGGTTGMRVGVAQAIHHTSHRSVFGTVLSEQPLMQNVLADLA
                     IESEAATISAVRLARAYDEAIAGDEDAAQLKRVANAVLKYWICKRAPMHAGESLECLG
                     GNGYVEESGMPRLFRESPLNSIWEGSGNVQCLDVLRAMIKEPASLEAFFAEVEEGAVA
                     EPRLAAFAQELRGDLPGEVETVQVRARALVERMAVALQGSLLVRFGDEAVAEAFVASR
                     LAGEWGQAFGTLPAGTDFARIIERHRPAA"
BASE COUNT          755 a         1804 c         1805 g          876 t
ORIGIN      
        1 tgggcgatct cgagcttgcg ctcggccgtc ttcgccatcc cgacctcgtc gatcgtcagc
       61 gcgatcagcg ccgcgccgtg cgccttcgcc agcgggatca ccgtgtcggc cttgtcccgc
      121 ccggcctcca aattgatcga gttgacgatc gcgcgcccgg ggatcgtctc gagcgccgcc
      181 tggatcacct cgggctcggt cgagtcgacc tggatcggtg ccggctgggt cagggagacg
      241 cgcttgacca cagcgctcat ttgctcgtcc tcgtcctggc gctcggtcag cgcgacgcag
      301 acgtcgagca cgtgggcgcc gccttcgacc tggtcttcgg cgacctggac caggccgtcg
      361 tagtcgtcgg cgagcagcag ttccttggct tttttcgagc cctgcgagtt gacccgctca
      421 ccgaccaggg tcgggcgggg ctcctgcacg agtggggtgg aggtcatcat cgaggagacc
      481 tcgatctgcc ctggcgcggg gcgctcggcc ggcaccttgc cgtcgacccg ggcgcggatc
      541 gcggcgatgt gcgctggcgt cgtcccacag cagccgccga cgatcccgac cccgtagcgc
      601 tcgacgaact cacccagcgt cgccgccagc ggctcgggct cttccgggaa gatcgtctcg
      661 ccgtcgggcc cctgcagggg caggccggcg ttggggatgc agtggaccgg cagcgggctg
      721 gtctcgccga ggaaacggat cgcgtcgcgc atgtcctcgg gcccggtcga gcagttgagc
      781 ccgaccacgt cgacgtcgag cgcggtcagc gtcgtcagca ccgactggat gtcggtgccg
      841 agcagcatct tgccgccgtt cggcagcagc gagacgctga tctgcagggg cagcgtgcgc
      901 ccggtctggg tgaacgcctc gcgggcgccg aagatcgccg ccttgacctc gaggatgtcc
      961 tgggcggtct cgatgatgat caggtcggcg ccgccttcga ccaggccggt cgcctgctcg
     1021 gcgaagactt cggccagctt cgcgaacgag atgtcgccga gggtcgggtc ggtggacgcg
     1081 ggcaggaacc ccgtcgggcc gatcgagccg gcgacgaagc ggtccgggcc ggccgccttg
     1141 cgggcgattt ccgcagcctt ggtgttgatc tccatggtct gctcgccgag gccccactcc
     1201 tcgagcttca gccgcgagcc ctggaaggtg tcggtctcga ccacctccgc gccagcttcg
     1261 agcatcgagg tgtggacccc ctcgatcacg tcggggcgat tcagaaccag cgcctcgtgg
     1321 catttcccga gcaggccgcc gtagtcctcc tgggtcaggt cgaacatctc cagggtcgcg
     1381 cccatgccgc cgtcgaagac gacgacgcgc tcccgcactg cggctaggta gtcgcgctca
     1441 ctcatgaacc taaagaggct accgagggcg cagccagttc atgcttctgt aggccgggac
     1501 gaaacctgcg cgcaacaaat tgcgcccggc aacggcgatc ccctcggcct cgctttcgcc
     1561 caactcggcg aagatcgtgt tgcagcccgc ctcgatcgcg tcgaggatcc gccgccgcag
     1621 cagcaccaga ttgcagccga acccgcgcgc ttcgggcagg gtcgccgcga ggccgacctg
     1681 ggcgacgtcg ccgtcgatca gcatcgagcc gaaggagacg atccgctcct cgagctcggc
     1741 cgtgtaggtg cgccagcgct cctggttcgg cagcccgaag agcaggctcg cagccttcga
     1801 agggaagccg agcgccggcg cggcgtcaaa gaccatcgtt tcgccggcgg cgtcttcgcc
     1861 gttgatctcc cagaccgtga tcgccgggtt gccgggcagg tccggccagg agacgtcgcg
     1921 cacgtacttc caggcgcccc ggccctgctc gaatccctgg tggttcaggt agttctccgc
     1981 ggccgcggtc ccggggcgat cgcgggcgac gctgacccgg tagccgacgt cgaactcgtc
     2041 ggcccaggcg atcgcgttcg ccagatggcc ttcctcgacc gcgccaggct cggcggcgcc
     2101 gagaatcgtg ttcatgcccg ggtcgtcggg cagcgcttcg aaggtcgtca cctgcaccgg
     2161 gccgaaccag gcttctgcga ttccgcactc cataacggcg tcttcgcagg ccgttcccca
     2221 catgtcggac cggaatgttt tcgccgtacg ctccagcgtc gcagcgtcgt actccatgcc
     2281 gttcccctct ccgatcgggg cctagccaac cccgaggtgg tcactctatg aggatgccct
     2341 ggatttgtaa aggaacttgt ttatctctgg aaaaaatccc accaaaagtt gctgggagcg
     2401 aacatcggag aacgcaagga ccctaaaact aagtagccag gtgctctaaa tcagcccttg
     2461 cggccgaggc ggcgcttcag gtcgcgggcg ttgaaggcca cggcgatccg gccgacgagg
     2521 ccgcggcggg cgcgggtgta ggggtaccag gtcggttcac gcttgccggc gaagcgggtg
     2581 atcacgaccg actcggtgcg gcagaacttc ttgattccgg gagcgccgtg gcgatagccg
     2641 attccggact ccttccagcc gcccatcggc acgtcggagg cgaagtagtt gaccagcacg
     2701 tcgttgacgt tgacggcgcc gcactcgacc cggcgggcga cctgctcggc ccgctgcttc
     2761 tcgccgaaga ccgagccgga gaggccgtag cggctgtcgt tggccagttt cagcgcttcc
     2821 tcggcgtcgc gtaccttcat caccccgacc acggggccga aggtctcgtc gcgcatcact
     2881 ttcatcgagt ggtcgacgtc gacgagcacg gtcggctcga agtagtcgcc ggggctctcg
     2941 acccgcttgc cgccggtcag cgcgcgggcg ccggccgcga gcgcgtcgtc gacgtggtcc
     3001 tcgacgatcc cgatctggtt gggcgaggtc atcgcgccga cgtcggcgtc gtggccgctc
     3061 gcgtcggggc cctggcgcag cttcccgact tcggcggtca gcttggcgac gaactcgtca
     3121 taggcgggct cctcgacgta gatccgctcg accgacatgc agatctggcc ggagttcatc
     3181 atcccgcccc aggccgcggc gttggcggcg cggtcgaggt cagcgtcggc gagcacgatc
     3241 atcgggtcct tgccgccgag ctcgaggctg accggggtca gcgtctccgc ggcgcgggcc
     3301 atcaccttgc gaccggtccg gtcggagccg gtgaactgga tgaagtcgac gttgtcgacc
     3361 agcgcgccgc cggtctcgcc gaccccctgg acgacgtcga agacgtcggg gccgccgatc
     3421 tcgtgcttcc aggcgtcgac gatctccgcc agcccgagcg gggtgaactc ggagggcttg
     3481 atcacgaccg cggccccggc ctgcagggcc ggcacggcat cgccgatcga gaggatcagc
     3541 gggaagttcc aggggctgat gatcccgacc accgggtgcg ggcggtactg gacgcgcagc
     3601 ttcttcgaag agaggagggc ggagtggggg cggaccgcct cctcgccgat gaacttggcc
     3661 gcgcggctcc cgtagaagtt gatgatgtcg gccaggtaga tcggctcgtt ggaggcgtcg
     3721 gcgcggacct tgccggtctc gcgctgcatc gtgtcgagga tccgctcctg gttgtcgagg
     3781 atccagtcgc gcagcttgcc gagccagtgg tagcggccct cgatccccat cgcctcccag
     3841 tcggcctggt tggcgcgcac gcgggcgacg gtcgccgcga cttcctcggg tgaagtgacc
     3901 gggacggtct gaatcagctg gccgttggcc ggattcagga cctcgatctg cttcggctcg
     3961 gcgccgctgc cggtgctggc gctgccgttc gtgccgatga tcttctccgc ggtgccgctc
     4021 tccactggaa tgccccctct ggttgaccca tgaccgtttg gctgctcagc caactttaga
     4081 ctgtgagccg ggatcgcgca cgaagacgca gtagttcccg cataccggga aaaactgcgt
     4141 tcgtgctcag gccgcggggc ggtggcgctc gatgatccgc gcgaagtcgg tgccggccgg
     4201 cagggtgccg aaggcctggc cccactcacc ggcgaggcgg gaggcgacga aggcctcggc
     4261 cacggcttcg tcgccgaagc ggaccagcag cgagccctgc agggcgaccg ccatccgctc
     4321 gaccagcgcc cgggcgcgga cctggaccgt ctcgacctcg ccgggcaggt ccccgcgcag
     4381 ctcctgggcg aaggcggcca gacgcggctc cgcgacggcg ccctcctcga cctcggcgaa
     4441 gaacgcctcc agcgaggccg gctctttgat catcgcccgc agcacgtcga ggcactggac
     4501 gttgccggag ccctcccaga tcgagttcag cgggctctcg cggaagaggc gcggcattcc
     4561 cgactcctcg acgtagccgt tgccgcccag gcactcgagc gactcgcccg cgtgcatcgg
     4621 cgcccgcttg cagatccagt acttgagcac cgcgttggcg actcgcttca gctgcgcggc
     4681 gtcctcgtcg ccggcgatcg cttcgtcgta ggcgcgggcc aggcgcactg ccgagatcgt
     4741 cgccgcctcc gactcgatcg cgaggtcggc gagcacgttc tgcatcagcg gctgctcgct
     4801 cagcacggtg ccgaagaccg agcggtgtga ggtgtggtgg atcgcctgtg cgaccccgac
     4861 ccgcatcccg gtggtaccgc cgagcacgca gtccaggcgc gtgtggttga ccatctcgat
     4921 gatcgtgcgc acgccggcgc cctcgtcgcc gaccagccag gccgaggcgt tgtcgaactc
     4981 gatctcgctg gaggcgttgg agcggttgcc gagcttgtcc ttcagccgct ggaggtggaa
     5041 ggcgttgcgg gtgccgtcgg ggcggaagcg ggggaggacg aagcacgaca gcccgtcctc
     5101 ggcctgcgcg agcaccagga aggcgtcgca catgggcgcc gagcagaacc acttgtggcc
     5161 gtggatctcg tactcgccgt cgccgatctc gaccgcccgc gtgacgttgg cgcgcacgtc
     5221 cgagccaccc tgcttctcgg
//
LOCUS       DASRIG010000295        11398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3009788, whole genome shotgun sequence.
ACCESSION   DASRIG010000295 DASRIG010000000
VERSION     DASRIG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009788"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFB52_08025"
     CDS             <1..872
                     /locus_tag="VFB52_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO06319.1"
                     /translation="ADLAMYDAKEQGRDTFAFFEPDAGHGPRIRARASWFERIEAAID
                     EDRFTLQAQPIVELGSGAVVQHELLIRMLDEQGDLIPPVTFLAVAERYGQIARIDAWV
                     ARRAVELLTELEAAGAARPLAINLSAHALGDRELLASLERQVSESGVDAANLVVEISE
                     GAAIADIGRARALAARLKALGCQVALDDFGAGVGSIYHLKHLPLDYIKIDGELVEDCA
                     RDERDRLAISAVVTVAQGLGVATVAEAVAEQDVADALREIGVDLAQGSCFGMPRDLAQ
                     ELAPATEDPEQVF"
     gene            980..2065
                     /locus_tag="VFB52_08030"
     CDS             980..2065
                     /locus_tag="VFB52_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06320.1"
                     /translation="MSKIVIAGASGVVGSAALERFLERDGWEAVALSRRRPDIDAARD
                     FDHVPVDLRDAGASRQVLGELEGVTHLLYTAVFEKPGLIAGWSEEDQMQTNLEMLENC
                     LAPLLEKGTLEQVTILQGTKAYGIHLHPMPVPARERYPRDDHPNFYWLQEDYLKEQAE
                     KHGFRWSILRPQLIIGSTIGAVMNLAPVIGAYAAIRKERSEPFSFPGGISYVWELVDS
                     RVCAAAAEFCATDPKADGQHYNVTNGEVFEWRNLWPALADQLGVETGPDEPIAMAEFF
                     PANEETWEKVVQSNGLRPNSLSDILGESHYYADFCFAYGAEEPPPPAFVSAIKLRKDG
                     VCETYDSEETFRHWIGWLQDRKILPAA"
     gene            2081..2713
                     /locus_tag="VFB52_08035"
     CDS             2081..2713
                     /locus_tag="VFB52_08035"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06321.1"
                     /translation="MSPHEAGGLVGRSTAALSVREQILLAALQCFLEKGYHGTTVRDI
                     ATKADLSVPGLYHHFKSKMELLEELMNDTMDDLRADVAGALSRARPDPVRRFEAVVEA
                     HVRFHCERQEESFLGNSELRSLPPKPLAQLVKKRDDVQEFFQVVIDEGIEAGIFRVGR
                     PHETARALASMCVAVASWYHRGGELSPDDVVAIYRDLGLQLVGFTDSRSA"
     gene            2922..3884
                     /locus_tag="VFB52_08040"
     CDS             2922..3884
                     /locus_tag="VFB52_08040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO06322.1"
                     /translation="MAANQQTLDELLSDLPKNWGRWGAEDEIGCLNFLTSEAVLRGLQ
                     EVQDGKVFTLGLPMGSPSGDPVWPGRAQATRLMTQDRSHYVNGRKQAMPGGLEYADDY
                     ITAFLQGSSQYDALGHTWYGDQIYNGFSADTTVGGMERCGVDKLAKKGVVGRGVLVDM
                     ARHRGKKALESGETFGLEDLLAAAESQGVTIEPHDNLVIRTGWLTIYYDEGPEAFFGE
                     SFVEPGLTFSPELVRWFHETEIVSLSTDTIANETTVDKESGIALPLHAALMRNLGVLF
                     SEILWLEDLAEDCAEDGRWSFLYAAAPIHIVEGTGAPVNPVVLK"
     gene            3895..5565
                     /locus_tag="VFB52_08045"
     CDS             3895..5565
                     /locus_tag="VFB52_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZO06323.1"
                     /translation="MSVYDEKPWLAHYPEGAPETIEIPFETGLEMFQATLERIPDAPA
                     IHYFDRAISFREIDEVSSALAVALQDLGFQRGDRLASYLQNVPQFPIAMLATWKAGGT
                     MVSVNPMLKGREAATILNDSGARALITLESLWRDVARDIVDDTPVSIPITTSELDFLG
                     DGETPALLAGCERDRDEATHDLVDLVSGNRGRAPEPSGLKADDIAFLTYTSGTTGPPK
                     GAMNSHANVVFNARSYQTWGDLGEQDVILAVAPLFHITGLIAHIGLSFLSGAPQVLFF
                     RFDPEAALELIEIHRATFTVASITVYISLMNAPGAGSRDVSSFTKILSGGAPIAPATV
                     EAYEGAMGAYIHNVYGLTETTSPSHFVPLGVKAPVDEESGALSVGLPIFNTMVRIRDD
                     EDNDLPAGEVGEIVTSGPQVVSGYWNKPEETEHAIPGGELHTGDVGVMDPDGWFYVVD
                     RKKDQINAAGYKVWPREVEDVVYGHEAVQEVAVIGVPDEYRGETVKAFVSLKPGAELS
                     DQELIAFCKERLAAYKYPRQIEFVDELPKTASGKVLRRELRDREASKA"
     gene            5647..7017
                     /locus_tag="VFB52_08050"
     CDS             5647..7017
                     /locus_tag="VFB52_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO06324.1"
                     /translation="MKSTTGGTSPRRSRRLLMLAVFAAFIAGIVLAACGGSDDSTGGS
                     ETAATEETTGGGGGGSSSAASKEFLEFTGISEKAKEALEGQEFKLGAILPLSGPGAAY
                     ATEEQNGILLAAEEMETALGLDVNYEVIDHKSGDPQAGAAAARQLGINGFGTAVNSYY
                     GVFGSTLPEIQKYKMLSLDPGGGTGNGLKGQEYFWGARANTPDDGMTALQYFKENEPE
                     RKKVAMVIWDAGAEYIDPIEEHLKEEVSRNGMTYTGTIKQKIGETDYSSVISDLRSMD
                     PDIIWLTSYGADPAYFMKQYVTSGLEAQVIGAEFTPAAAEIAGSAYDEFDYANDFFNF
                     DEPSSAFAEYFMKQYEEKFGETPNIFYEPNYYEVGVVYAVLAARVAEKGGDINSGEEL
                     NKALEENPEFPSVYGGSGSEVGKLVFNKETHDPEVRPVALVRATEPPEVLAEWNIGGT
                     EFKTVK"
     gene            7099..8076
                     /locus_tag="VFB52_08055"
     CDS             7099..8076
                     /locus_tag="VFB52_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZO06325.1"
                     /translation="MSSQIFAAILGIDSFRELAQAMVTGIITGAAYGLLGAGLALILG
                     VTGRFHFAVGSTYMVTAYLAAVATGIWGLPLIPAVIVGLAVATVFALAIEGVVYNPLA
                     KRAGDGGLLPVFVASLGIVIITENLVRVVWGNNTRNLEGFPSHTYNLGNVTFTLLDLT
                     LVIVTLVLLGGLSLLLARTVLGEQIRAVRGNPLMAQAVGVSVRRIFYLVFAIGTLVVG
                     VAAIFSAMKFAVIPSMGNEPIFYAFVVAFVAGSHRPPIVVGLVGIAIGLAESVSTIWV
                     SDNISALVVFGLLFAILAARSVPQGIRQLSGALSKANRRTLERSAQTRA"
     gene            8080..9819
                     /locus_tag="VFB52_08060"
     CDS             8080..9819
                     /locus_tag="VFB52_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZO06326.1"
                     /translation="MYWIDVCNQILIFATLAMSLNLLLGYAGQVSVAHAAFAAIGGYA
                     SGYLALKSGVSVPLAFAIGILGAGLLGIVVSLPALLLSGEYMILLTLSVQTIVLIVIT
                     SIGALGGLYGLTGLPTVSFFGKELLRPEDWLVPLIIVNVVILGLLTWIGESPFGRILR
                     GIRDDQLATQSLGKDVWVKKIIVFGLTSAIAGLGGALLAYYNGVTAPGLFEFNQSILI
                     VTMVVVGGRGNLVGSILGAAVVIGAGPFFEKVINLSPETSALWRLIAFGVLLIVVLML
                     RPQGLLPEGLSIQALIRKLRSRSAVEEPVVAPAPVPASTPGNGTAPAGDGGEPILKVS
                     DVRKSFGGIHAVNGLSFELPRGRVTGLIGPNGAGKTTVFNLLTGAITLDEGTVELRGE
                     DITGWPIDRVARHGMVRSFQDVRIFPSLTVLDNVRMAVQQQRGESLRDLFLMPWSVVR
                     DQRRTIRRAREKLGFVGLADKENQLAGSLPFGEQKLLALARILAADAEIFLLDEPASG
                     IDAEWVDQMVEIIRRLADSGMTVCIVEHNLHVVERVADNVYFMDGGQIVAEGTMEDLV
                     KAENLVEAYFGQP"
     gene            9806..10531
                     /locus_tag="VFB52_08065"
     CDS             9806..10531
                     /locus_tag="VFB52_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06327.1"
                     /translation="MASPEKSPTLLEIESLRAGYGRTAVVFDVNLTVNRGEIIAILGH
                     NGAGKTTTLKTIFGLLKPLGGSMRYAGENLAAQSYTKRAEGGLSFTPAERFVFPDLSV
                     AANLRLGAIGVKSKDTREHQRKLVHDLFPLLDERASQLAGTLSGGQQRILSIGIALMS
                     DPKLLLLDEPSLGVSPKITQEILTAMRRMANEEGRSVILVEQNVAYALREAERVYVMR
                     SGRTILEASASEMRKREHWWDLF"
     gene            10560..>11398
                     /locus_tag="VFB52_08070"
     CDS             10560..>11398
                     /locus_tag="VFB52_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZO06328.1"
                     /translation="MSSLDQDARTDDVVRTWAEAERAELPPLIVLEGLEPFVPGKGEI
                     TVKRLGTGHSNETFMVTRDGASMVLRRPPRPPIPPTAHDVVRESNILTALAEHSVRAP
                     VPLVTHAEDDAIGAPFYLMELVPGVVIRESMPPLLDSPEGRRGAVREFVAAVAEVHAV
                     PWQGTALEKIGRPSGYLERQLRRWNSQWEHNQTRVVPEIVEVGEWLAANRPTAEETTV
                     VHGDAKLDNAIYREGMPVRLEALVDWEMATLGDPLADLGLLCGTYVEPGEEPDPVLGF
                     SPAT"
BASE COUNT         1989 a         3874 c         3760 g         1775 t
ORIGIN      
        1 acgccgacct ggcgatgtac gacgccaagg aacaggggcg cgacaccttc gccttctttg
       61 agcccgacgc cggccacggt ccacggatcc gtgcccgggc gagctggttc gagcggatcg
      121 aagcggcgat cgacgaggat cgcttcaccc tgcaggcaca gccgatcgtc gagctcggca
      181 gcggcgccgt cgtccagcac gagctgctga tccggatgct cgacgagcag ggggacctga
      241 tcccgcccgt gaccttcctc gcggtcgccg agcgctacgg ccagatcgcc aggatcgacg
      301 cctgggtggc gcggcgggcg gtcgagctgc tgaccgagct cgaggcggcc ggcgcggcgc
      361 ggccgctggc gatcaacctc tcggcgcacg cgctcggcga ccgcgagctg ctcgccagcc
      421 tcgaacgcca ggtctccgag tcgggggtcg acgcagccaa cctcgtggtc gagatcagcg
      481 agggtgccgc gatcgccgac atcggccgcg ctcgcgcgct cgctgcgcgg ctgaaggcac
      541 tcggctgcca ggtcgcgctc gacgatttcg gcgccggcgt cggctcgatc taccacctca
      601 agcacctgcc actcgactac atcaagatcg acggcgagct agtcgaggac tgcgcccgcg
      661 acgagcgcga tcgcctcgcg atcagcgcgg tggtcacggt ggcccagggc ctcggcgtcg
      721 ccaccgtcgc cgaggcggtc gccgagcagg acgtcgcaga cgccctgcgg gagatcggcg
      781 tcgacctcgc ccagggcagc tgtttcggga tgccgcgcga cctcgcgcag gagctggcgc
      841 ccgccaccga ggaccctgag caggtgttct gaccggtcgc ggggccggat gccgatcgtt
      901 gacttgggcg caccccttca gctagtgtcc gagcgagcgc tcggtcggag ccgggcatca
      961 acttgggaag gagcgaggta tgagcaagat cgtgatcgcg ggtgcgagtg gggtcgtcgg
     1021 ttcggcggca ctggaacgct tcctcgaaag ggacggatgg gaagcagtcg cactgtcgcg
     1081 ccggcgcccc gacatcgatg cggcacgcga cttcgaccac gtgccggtcg acttgcgcga
     1141 cgcaggcgct tcgcgccagg tcctcggaga gctcgagggc gtcacgcacc tgctctacac
     1201 cgcggtcttc gagaaaccgg gactgatcgc tggttggtca gaagaggacc agatgcagac
     1261 gaacctggag atgctcgaaa actgcctggc cccgctgctc gagaagggca ccctcgagca
     1321 ggtgacgatc ctgcagggga ccaaggccta cggcatccac ctgcacccga tgccggtacc
     1381 agcacgcgag cgctacccgc gggacgatca tccgaacttc tactggctgc aggaggacta
     1441 tctgaaggag caggccgaga agcacggatt ccgctggtcg atcctgcgcc cgcagctgat
     1501 catcggcagc acgatcggcg cggtgatgaa cctggcgccg gtaatcgggg cctacgcggc
     1561 gatccgcaag gagcggagcg agccgttctc cttccccggc ggcatctcct atgtctggga
     1621 gctggtcgac tcgagggtct gcgccgccgc ggccgagttc tgcgccaccg atccgaaggc
     1681 cgacggccag cactacaacg tcaccaacgg cgaggtgttc gagtggcgca acctctggcc
     1741 ggcgctcgcg gatcagctcg gggtcgagac cgggcccgac gagccgatcg cgatggccga
     1801 gtttttcccc gcaaatgagg agacctggga gaaggtcgtg cagagcaacg gcctgcggcc
     1861 caacagcctc agcgacatac tcggtgagtc gcactactat gccgatttct gtttcgcata
     1921 cggagccgaa gagccgccgc cgccggcctt cgtgagcgcc atcaagctgc gaaaggacgg
     1981 cgtctgtgag acctacgaca gcgaggaaac cttccggcac tggatcggtt ggctccagga
     2041 tcgaaagatc ctccccgctg cctgagcggg gaggatccag atgagtcccc acgaggcggg
     2101 aggtctcgtc ggtcgttcca cggcggcgct gtcggtgcgg gagcagatcc tgctggcggc
     2161 gctgcagtgc ttcctggaaa agggctacca cgggacgacc gtgcgggaca tcgccaccaa
     2221 ggcggacctc agcgtgccgg gtctctacca ccacttcaaa tcgaagatgg agctcctgga
     2281 agagctgatg aacgacacga tggacgatct gcgcgcggat gtcgccggcg cgctctcccg
     2341 ggcgcggccg gacccggtgc ggcgcttcga agcggtggtc gaggcgcacg tccgcttcca
     2401 ctgtgagcgc caggaggaga gcttcctcgg caactccgag ctgcgcagcc tccccccgaa
     2461 gccgttggcc cagctcgtca agaagcgcga cgacgtccag gagttcttcc aggtggtgat
     2521 cgacgagggc atcgaggccg ggatcttccg ggtcggtcga cctcacgaga ctgcccgagc
     2581 gctcgcctcg atgtgcgtcg cggtcgcctc ctggtaccac cgaggcggcg agctctcccc
     2641 cgacgacgtg gtcgcgatct accgcgatct cggcctccag ttggtcgggt tcaccgactc
     2701 gcgcagcgcc tagaagccac ctcgttgcag cgctgagtcg ccgtgcctcc ctcgcgggga
     2761 gcgccaggcg acgcgcgcct gaaagttcgg aaatcagctc ttgactttcc gaaaacgccg
     2821 tgcataggat ggagcgaccg agcgctcgct cggtagggac ctacgacttc aacgcaagcg
     2881 accggtcggt gacgacggtc gatgggaacg gagagattga gatggctgca aaccagcaaa
     2941 cgctcgacga gctcctgtcg gacctgccga agaactgggg ccggtggggg gcggaggacg
     3001 agatcggctg tctgaacttc ctcacctcag aggcggtcct tcgcggactg caggaggtcc
     3061 aggacggcaa ggtcttcacg ctcggcctgc cgatgggcag cccgagcggc gacccggtgt
     3121 ggccgggccg ggcccaggcg actcgcctga tgacccagga ccgcagccac tacgtcaacg
     3181 gccgcaaaca ggcgatgccg ggcgggctcg agtacgccga cgactacatc accgccttcc
     3241 tgcagggctc gagccagtac gacgcgctgg gccacacctg gtacggcgat cagatctaca
     3301 acggcttctc ggcggacacg acggtcggcg gaatggagcg ctgcggggtc gacaaactcg
     3361 ccaagaaggg cgtcgtcggc cgcggcgtgc tggtcgatat ggcgcgtcac cgcggcaaga
     3421 aggccctgga aagcggcgag accttcgggc tggaggacct cctcgccgcc gctgagtccc
     3481 agggcgtcac gatcgagccc cacgacaacc tggtgatccg caccggctgg ctgacgatct
     3541 actacgacga gggccccgag gccttcttcg gcgagagctt cgtcgagccc gggctgacct
     3601 tcagccccga gctcgtgcgc tggttccacg agaccgagat cgtctcgctc tcgaccgaca
     3661 cgatcgccaa cgagacgacg gtggacaagg agagcgggat cgcgctgccg ctgcacgccg
     3721 ctctgatgcg caacctcggc gtcctcttct cggagatcct ctggctcgag gacctcgccg
     3781 aggactgcgc cgaggacggc cgctggtcct tcctctacgc cgccgccccg atccacatcg
     3841 tcgagggcac cggcgccccg gtcaaccccg tcgtcctcaa gtaggggagt cacgatgagc
     3901 gtctacgacg aaaagccctg gctggcgcac tacccggagg gagcccccga gacgatcgag
     3961 atcccgttcg agacggggct cgagatgttc caggccactc tcgagcggat cccggacgcg
     4021 ccggcgatcc actacttcga ccgcgcgatc agcttccgcg agatcgatga ggtctcgagc
     4081 gctctggcgg tcgccctgca ggacctcggc ttccagcgcg gcgatcggct cgcctcctac
     4141 ctgcagaacg tgccgcagtt cccgattgcg atgctcgcga cctggaaggc gggggggacg
     4201 atggtctcgg tcaacccgat gctgaaaggc cgcgaggccg cgaccatcct caacgactcc
     4261 ggcgcccgtg ccctgatcac cctggagtcg ctgtggaggg acgtcgcccg ggacatcgtc
     4321 gacgacacgc cggtctcgat cccgatcacg acctccgagc tcgacttcct gggagatggc
     4381 gagactcccg cgctgctcgc cgggtgcgag cgcgaccgcg acgaggcgac ccacgacctc
     4441 gtcgacctcg tctcgggcaa ccgcgggcgg gctccggagc cgtccggcct caaggccgac
     4501 gacatcgcct ttctcaccta cacctccggc accaccggcc cgccgaaggg ggcgatgaac
     4561 agccacgcca acgtcgtctt caacgctcgc agctaccaga cctgggggga cctcggcgag
     4621 caggacgtga tcctcgcggt cgcgccactg ttccacatca ccgggttgat cgcccacatc
     4681 gggctcagct tcctctcggg cgcaccgcag gtgctcttct tcaggttcga ccccgaggcc
     4741 gcgctagagc tgatcgagat tcaccgtgcg accttcacgg tcgcctcgat caccgtctac
     4801 atctcgctga tgaacgcccc cggcgccggg tcccgggacg tctcctcctt cacgaagatc
     4861 ctcagcggag gggcgccgat cgcacccgcg acggtcgagg cgtacgaagg tgcgatgggc
     4921 gcctacatcc acaacgtcta cggcctcacc gagaccacct cgccctcgca tttcgtcccg
     4981 ctcggcgtca aggccccagt cgacgaggag tccggggcgc tctcggtcgg gttgccgatc
     5041 ttcaacacga tggtccggat ccgcgacgac gaggacaacg acctgcccgc gggcgaggtc
     5101 ggggaaatcg tcacctcggg cccccaggtc gtctccggct actggaacaa accggaggag
     5161 accgagcacg cgatcccggg cggcgaactg cacaccggcg acgtcggcgt gatggaccct
     5221 gacggttggt tctacgtcgt cgaccgcaag aaggaccaga tcaacgcggc cggctacaag
     5281 gtctggccgc gcgaggtcga ggacgtcgtc tacggacacg aagcggtcca ggaggtcgcc
     5341 gtgatcggcg tgcccgacga gtaccgcggc gaaaccgtga aggcgttcgt cagcctcaag
     5401 cccggcgcag agctgagcga ccaggagctg atcgccttct gcaaggagcg cctggccgcc
     5461 tacaagtacc cgcgccagat cgagtttgtc gacgagctgc cgaaaacggc gagcggcaag
     5521 gtcctgcggc gagagctgcg cgaccgtgag gcgagcaagg cctaggcaat gggccgaatt
     5581 cgtcctgatg ttgggaacga accgtctcgc gatcgcgagg cacaaatggg aatggagaga
     5641 aacagcatga aatcgaccac gggagggact tccccccgtc gcagccgacg cctgctgatg
     5701 ctcgcggtgt tcgccgcttt catcgccgga atcgtcctcg ctgcgtgtgg ggggagcgac
     5761 gacagcaccg gcgggagtga aaccgcagcc accgaagaaa ccaccggcgg cggaggcggc
     5821 ggttcctcga gcgccgcgtc caaagaattc ctcgaattca ccggcatctc cgagaaagcc
     5881 aaggaagcgc tggaaggcca ggaattcaag ctgggggcga ttctgccgct gagcggaccc
     5941 ggcgccgcgt acgcgaccga agagcagaac ggcatcctgc tcgccgccga ggagatggaa
     6001 acggcgctcg gcctcgacgt caactacgaa gtgatcgacc acaagtcggg cgatccccag
     6061 gccggcgcgg cagccgcacg ccagctcggg atcaacggct tcggaaccgc ggtcaactcc
     6121 tactacgggg tcttcggctc caccctgccg gaaatccaga agtacaaaat gctctcgctc
     6181 gatccgggcg gcggaaccgg caacggtctc aagggccagg aatatttctg gggcgcgcgg
     6241 gcaaacacgc ctgacgacgg catgaccgcc ctgcagtact tcaaagaaaa cgaacccgag
     6301 cgcaaaaagg tggcgatggt catctgggac gccggcgccg aatacatcga cccgatcgaa
     6361 gaacacctca aggaagaggt gtcgcgaaac gggatgacct acaccggcac gatcaaacag
     6421 aaaatcggcg agaccgacta ctcgtcggtg atctccgatc tgcgctccat ggacccggac
     6481 atcatctggc tcacctcata cggggccgat ccggcgtact tcatgaaaca gtacgtgacc
     6541 tccggcctcg aggcacaggt gatcggtgcc gagttcacgc ccgcggctgc tgaaatcgcg
     6601 gggtccgcgt acgacgagtt cgactacgcg aacgacttct tcaacttcga cgaaccgagc
     6661 agcgcgttcg ccgaatactt catgaagcag tacgaagaga agtttggtga gacgccgaac
     6721 atcttctacg agcccaacta ctacgaagtt ggcgtcgtgt acgcggttct cgcagcccgg
     6781 gtcgccgaaa aaggcggcga catcaacagc ggcgaagaac tgaacaaagc actcgaagaa
     6841 aacccggaat tcccgagtgt gtacggcggc agtggatctg aggtcgggaa actggtcttc
     6901 aacaaagaga cccacgatcc cgaagtacgc cccgttgcgc tcgtccgggc gaccgaaccc
     6961 cctgaagtgc tggccgaatg gaacatcggc ggcaccgaat tcaagacggt gaaatagccg
     7021 ccgcttgaag ccggctgcgg tcgggcgggt acgtctcctc gcccgtccgg ccgctgcctc
     7081 cccacggacc tccttgacat gagctcacaa atcttcgcag cgatactcgg gatcgattcc
     7141 ttccgggaac tcgcccaggc gatggtcacc gggatcatca ccggtgccgc ctacgggctg
     7201 ctcggcgcgg gcctggcgtt gatcctcggc gtcaccgggc gctttcactt cgccgtcggc
     7261 tcgacctaca tggtcaccgc ctacctggcg gccgtcgcca ccgggatctg gggcctgccg
     7321 ctgattcccg ccgtgatcgt cggcctcgcg gtggcgacgg tcttcgccct cgcgatcgag
     7381 ggcgtcgtct acaaccccct cgcaaagcgc gccggcgacg gcgggctgct gccggtcttc
     7441 gtcgcctcgc tcgggatcgt gatcatcacc gaaaacctgg tgcgcgtcgt ctggggcaac
     7501 aacacgcgga acctcgaagg cttccccagt cacacctaca acctcggcaa cgtcaccttc
     7561 acgctgctcg acctgaccct ggtgatcgtc accttggtgc tgctcggcgg cctctcgctg
     7621 ctgctcgcgc gcacggtcct gggcgagcag atccgcgcgg tgcgcggcaa cccgctgatg
     7681 gcgcaggcgg tcggcgtcag cgtgcgccgg atcttctacc tggtgttcgc gatcgggacc
     7741 ctggtcgtcg gcgtcgcggc gatcttctcg gcaatgaaat tcgccgtgat cccgagcatg
     7801 ggcaacgaac cgatcttcta cgccttcgtc gtcgccttcg tcgcgggctc gcaccgacca
     7861 ccgatcgtcg tcgggctggt cgggatcgcg atcgggctcg cggagagcgt gagcacgatc
     7921 tgggtcagcg acaacatcag cgccctcgtc gtcttcggtc tgctgttcgc gattctcgcc
     7981 gctcgctcgg tcccgcaggg gatcaggcag ctctcaggcg ctctgtccaa agccaaccgc
     8041 cgcacgctcg aacgttcggc ccagacccgc gcctagccca tgtactggat cgacgtctgc
     8101 aaccagatcc tgatcttcgc caccctggcg atgtcgctga acctgctgct cggctacgcc
     8161 ggccaggtct cggtcgccca cgcagccttc gcggcgatcg gcggctatgc ctccggctac
     8221 ctggcgctga aaagcggcgt ctcggtcccc ctcgccttcg ccatcgggat cctcggcgcc
     8281 ggcctgctcg ggatcgtggt tagcctcccg gccctgctcc tgagcggcga gtacatgatc
     8341 ctgctgaccc tctcggtgca gacgatcgtg ctgatcgtga tcaccagcat cggcgcgctc
     8401 gggggcctct acgggctcac cggtctgccg acggtcagct tcttcggcaa ggaactgctg
     8461 cgccccgaag actggctcgt acctctgatc atcgtcaacg tggtcatcct cggactgctg
     8521 acctggatcg gggagtcgcc gttcgggcgc atcctgcgcg gcatccgtga cgatcagctg
     8581 gcgacgcagt cgctgggcaa ggacgtctgg gtgaagaaga tcatcgtctt cggcctgact
     8641 tcggcgatcg ccgggctggg cggcgccctg ctcgcctact acaacggcgt caccgctccc
     8701 gggctgttcg aattcaacca atcgatcctg atcgtgacga tggtcgtggt cggcggccgc
     8761 ggaaacctgg tcgggtcgat cctcggtgcc gccgtcgtga tcggcgccgg gcccttcttc
     8821 gaaaaggtga tcaacctctc gccggaaacc tcggccctct ggcggctgat cgctttcggc
     8881 gtcctgctga tcgtggttct gatgctgcgc ccgcagggcc tgctgcccga agggctcagc
     8941 atccaggcgc tgattcgcaa gctgcgcagc cgcagtgctg tggaggagcc ggtcgtggcg
     9001 ccggccccgg tgccggcgtc gaccccgggg aacggaacgg cccccgccgg cgacggcggg
     9061 gagccgatcc tcaaggtctc cgacgtgcgc aaatccttcg gcggcatcca cgcggtcaac
     9121 gggctcagct tcgagctgcc caggggtcgg gtcaccgggc tgatcggtcc caacggcgcg
     9181 ggcaagacga cggtcttcaa cctgctcacc ggcgcgatca cgctcgacga gggtacggtc
     9241 gagctgcgcg gcgaggacat caccggctgg ccgatcgacc gggtcgcccg ccacggcatg
     9301 gtgcgctcct tccaggacgt tcgcatcttc cccagcctca cggtgctcga caacgtccgc
     9361 atggcggtgc agcagcagcg cggcgagagc ctgcgcgacc tcttcctgat gccatggtct
     9421 gtggtccgag accagcgccg gacgatcagg cgggcacggg agaaactcgg cttcgtcggg
     9481 ctcgccgaca aggagaacca gctggccggc tcgcttccct tcggtgagca gaaactgctg
     9541 gcgctggcgc ggatcctcgc tgccgacgcg gagatcttcc tgctcgacga gccggcgtcg
     9601 gggatcgacg cggagtgggt cgaccagatg gtcgagatca tccgccgcct cgccgacagc
     9661 gggatgacgg tctgcatcgt cgagcacaac ctgcacgtgg tcgaacgggt cgccgacaac
     9721 gtgtatttca tggacggggg ccagatcgtc gccgagggca cgatggagga cctggtcaag
     9781 gcggagaacc tcgtggaggc ttactttggc cagccctgag aaaagcccaa ccctgctcga
     9841 gatcgagtcc ctgcgggccg gctatggccg caccgcggtg gtcttcgacg tcaacctcac
     9901 cgtcaaccgc ggcgagatca tcgcgatcct cggccacaac ggcgccggca agacgacgac
     9961 gctgaagacg atcttcgggc tgctgaagcc gctcggcggg agcatgcgct acgcgggaga
    10021 gaacctcgcg gcgcagtcct acaccaagcg cgccgaagga ggactctcct ttacgccggc
    10081 cgagcgcttc gtcttccccg acctctccgt cgcggccaat ctccgcctcg gtgcaatcgg
    10141 ggtcaagagc aaggacacgc gcgagcacca gcggaagctc gtccacgacc tcttccctct
    10201 gctcgacgag cgcgcctccc agctcgccgg aaccttgagc ggcggccagc agcggatcct
    10261 cagcatcggg atcgcgctga tgtcggaccc gaagctgctc ctgctcgacg agccctcgct
    10321 cggggtctcg ccgaagatca cccaggaaat cctcacggcg atgcggcgga tggccaacga
    10381 ggaaggccgc tcggtgatcc tcgtcgagca gaacgtggcc tacgcgctgc gcgaggccga
    10441 gcgggtctat gtgatgcgct cgggccgaac catcttggaa gcctccgcga gcgagatgcg
    10501 caagcgcgag cactggtggg atctcttctg atggcgccaa agacgattcg gagggagcca
    10561 tgagctccct cgatcaggat gcgcgcaccg acgacgtggt gcggacgtgg gccgaggccg
    10621 agcgggccga gctgccgccg ttgatcgtcc tcgaggggct cgagccgttc gttccgggca
    10681 agggggagat caccgtcaag cgcctcggca ccggccactc caacgagacc ttcatggtca
    10741 cccgggacgg tgccagcatg gtcctccggc gcccgccccg gccgccgatt cccccgacgg
    10801 cccacgacgt ggtgcgcgaa agcaacatcc tcaccgcgct cgccgagcac agcgtccgcg
    10861 cgccggtgcc gctcgtcacc cacgccgagg acgacgcgat cggcgcgccc ttctacctga
    10921 tggagctggt ccccggcgtc gtgatccgcg agagcatgcc gccgctgctc gacagcccgg
    10981 agggccgtcg cggcgcggtg cgggagttcg tcgccgcggt cgccgaggtg cacgccgtgc
    11041 cctggcaggg cacggccctg gagaagatcg gccgaccgag cggatatctc gaacgccagc
    11101 tgcgccgctg gaacagccag tgggagcaca accagacccg ggtcgtgccc gagatcgtcg
    11161 aggtcgggga gtggcttgcc gccaaccgcc cgaccgcgga agagaccacg gtcgtgcacg
    11221 gagacgccaa gctcgacaac gcgatctacc gcgagggaat gccggtgcgc ttggaggcgc
    11281 tggtcgactg ggagatggcg accctgggag atccgctcgc cgacctcggc ctgctctgcg
    11341 gcacctacgt cgagcccgga gaagaacccg accccgtcct cggcttctcc ccggcgac
//
LOCUS       DASRIG010000296         6983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_677544, whole genome shotgun sequence.
ACCESSION   DASRIG010000296 DASRIG010000000
VERSION     DASRIG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677544"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1314)
                     /locus_tag="VFB52_08075"
     CDS             complement(136..1314)
                     /locus_tag="VFB52_08075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06329.1"
                     /translation="MSELATSASLPLVETDEHKLIRESVSNIANSFGHRYFAEVVAKD
                     ESPHELWQALSEHGFMGPAIATEYGGGGTGLLELAIVEEELSAAGLPLLKMLVSPAVV
                     GAIIERHGSEEQKQRWLPGMAAGTERFSLAVTEPDAGSNTHKISTRARRRGEGYVLTG
                     TKTYSSGLEDSDWVLVLANSGEDEDGRAQLSLFVVEADHPQMERFRIPVVLRAPEHTF
                     TVMFDEVEVPADRLIGVEGEAIKTMFDGLNPERIMSSALCCGVGRYALEKASAYANER
                     SVWGQPIGAHQGVSHPLAEAKINLEAARLMMMKAAVLHDARGPAGEAANMAKLLGADA
                     GVRALDQSIQAHGGNGLAEEYGLADLYFIVRLQRIAPVSREMILNFVAQHSLGLPRSY
                     "
     gene            1402..2157
                     /locus_tag="VFB52_08080"
     CDS             1402..2157
                     /locus_tag="VFB52_08080"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06330.1"
                     /translation="MSAELAGKSGIVTGGARGIGAAIAAAFAEAGARVAVFDQDGDGA
                     DATAAEIGGGAISREVDVTDEDVVAAAVNDVFEAFGSIDFLVNNAGVRHVAPVAEHPV
                     DVWRRTIEINVVGTFICSQAVIRVAREHGGCRILNLGSMAGELALGERSAYNASKGAV
                     EALTKSIAVDHGEEGIYCNSIAPGVIETPLSAPYFEDEAMVKVLRENSPIGRWGQVAE
                     VAAPAVFLCSDAASFIQGETLFVDGGWTAGKGY"
     gene            2157..2909
                     /locus_tag="VFB52_08085"
     CDS             2157..2909
                     /locus_tag="VFB52_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06331.1"
                     /translation="MLDSGLEGRVCLVTGAASGIGLASARILAGEGAKLALWDLDEGA
                     LEGADLDDGGSATVSRSAVDIADPDAVRAGVEAIVAAHGTVDALIHCAGIYTTAPVPE
                     ITSEDWNRVLDVNLRGAFNVSQAVAEPMREHGDGRIVLFGSFAARTGGLRASAPYAAS
                     KGGIAGLTKHLAQWGGPLGIRVNCIHPGFIQTPMNAILSDEAKQQAVDNTPLRRLADP
                     PECAAMAVVLASDLASFVHGVELDVNGGMYMA"
     gene            2906..4459
                     /locus_tag="VFB52_08090"
     CDS             2906..4459
                     /locus_tag="VFB52_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06332.1"
                     /translation="MSIDWMGNRSLTDLLQERLALSPDDTFLVFENRAGEVSELTYAE
                     FETRVEDAARGLNALGVEAGGRVVVHLRNSPEFLISWFAIVRLGATMVPSNIANTAGE
                     LEHVVGFTEASHAITEPDFCDAVQRGIELAGTDTTTIVARGKLAGFFAFDDLATLSSE
                     PAPRPPVSGDDVAELLFTSGTTSKPKAVMLTHANCLRAGLDTVHCLWLDRGERMLTAL
                     PLFHVNGQGMTILAAMTVGGTAIVIEEFSASKFWKQVKRHKATQTAVVAMQLRVLIAQ
                     PDDPSDREHDVRKLFYAINVSDQEKEAFEERFGIEVINGYGLSEAMTLCTVSPVAAPR
                     RWPSIGLPSPGRTLLLLDEDGNEVPTGEVGEICVKGIPGRSIMLGYYKDEQSTAQAIR
                     GDLLHTGDNAYADEQGYLYFFDRKKDMIKRAGENVSAIEVESVLNDHPQIAEAAIVGM
                     PDPIRDEAVAAVIVVAEGAELSEEAVVAYCSERLSRFKVPTIIAFEAELPKTSIGKVR
                     KDELRKKLA"
     gene            complement(4540..5145)
                     /locus_tag="VFB52_08095"
     CDS             complement(4540..5145)
                     /locus_tag="VFB52_08095"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06333.1"
                     /translation="MQETKTDPGSAPTVTVRADIIEAATRIFSERGYHAASMAEIADA
                     VGIRKASLYHHVRKKEDLLFAIHETLIDELISETIAVSSSSESPAEKVRGLLHVTMRF
                     IARNRESVTVFLSERHAVTGERWQALVIKRDFYERMVSQIISDGRSSGAFADLPPEIV
                     TKALLGMANWGYTWFQQDGPLSADEVAEVFTAIALRGLEAP"
     gene            5304..6275
                     /locus_tag="VFB52_08100"
     CDS             5304..6275
                     /locus_tag="VFB52_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZO06334.1"
                     /translation="MGMTTRDETTDAERLARYRLMRLSRRFEETVHEQFHDGNVPGPL
                     HLSIGQEAVAIGGCWPLRETDVIVSTHRGHHHCLAKGARADRMMAELLGRADGYSRGR
                     GGSMHIAIPEIGLLGTNGIVGGGLPIGTGAALGLQIDGRDDVAVSFFGEGAAANGVFG
                     EALNTASLWNLPIVFICENNRYVELTPQHVHVAGEIWKRAEGYDMPGVKVDGNDVDAV
                     VETVAAAVDRARAGDGPTLIEAETYRWFGHYAGDKAAYRDEAEVEAGRAGDPLVRTRA
                     LLDPVEADLAEAEVEKEIAAALEFALASPIAGEEVLTPGHYAEEVSA"
     gene            6272..>6983
                     /locus_tag="VFB52_08105"
     CDS             6272..>6983
                     /locus_tag="VFB52_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO06335.1"
                     /translation="MSTAAVETRSMKFWQGVNQALVEEMDRDERTFLVGEDVARVGGP
                     YGITRGLLDRYGPSRVRDTPISESLLAGLGTGAAAVGMRPIVEIMFFDFAMLAMDQIV
                     NQAAKYRYFTGHSLPLTIRTMCGAGGPNGAQHSQNFEAWFCAVPGLKVVMPSNAADAK
                     GMLKSSIRDEDPTLFIETQGLLTARAEVPLEEDYLVPLGQAAIPREGSDVTVVAIGRM
                     VDRALEAAEQLAADGISVE"
BASE COUNT         1078 a         2434 c         2440 g         1031 t
ORIGIN      
        1 ccagcgcctc cttgtcgggg tagctccagg tgatccgctc ccacatgtcg ccgacgctga
       61 cgcggtccca gcggtcgctc gctcggcggt tggtgaggtt ggtgacgtcg atcgccacgg
      121 gcactccctt ctcggttagt aggacctcgg cagcccgagc gagtgctggg cgacgaagtt
      181 gaggatcatc tctcgcgaaa ccggggcgat ccgctgcagg cggacgatga agtagaggtc
      241 ggcgaggccg tactcctcgg ccagcccgtt gccgccgtgg gcctggatcg actggtcgag
      301 ggcgcgcacg ccagcgtcgg cgccgagcag cttcgccatg ttggcggcct ccccggccgg
      361 gccgcgggcg tcgtgcagga cggccgcctt catcatcatc agccgcgccg cctcgaggtt
      421 gatctttgct tccgcaagtg ggtgggagac gccctggtgg gcgccgatcg gctggcccca
      481 gacgctgcgc tcgttggcgt aggccgaggc cttctcgagc gcgtagcggc cgaccccgca
      541 gcagagcgcc gaggacatga tccgctccgg gttgaggccg tcgaacatcg tcttgatcgc
      601 ctcgccctcg accccgatca ggcggtcggc ggggacctcg acctcgtcga acatcaccgt
      661 gaaggtgtgc tcgggggcgc gcagcacgac cgggatccgg aagcgctcca tctgcgggtg
      721 gtcggcctcg accacgaaca gcgagagctg ggcgcgaccg tcctcgtcct caccggagtt
      781 ggcgagcacg aggacccagt cgctgtcctc cagccccgag ctgtaggtct tggtcccggt
      841 gagcacgtag ccctcgccgc ggcgccgggc ccgggtggag atcttgtggg tgttggagcc
      901 ggcgtcgggc tcggtcacgg cgagcgagaa gcgctcggtc ccggccgcca tgcccggcag
      961 ccagcgctgc ttctgctcct cgctgccgtg gcgttcgatg atcgcgccga cgaccgcggg
     1021 ggagaccagc atcttcagca gcggcagccc ggccgcgctc agctcttcct cgacgatcgc
     1081 cagctcgagc aggccagtgc cgccgccgcc gtactcggtg gcgatcgcgg ggcccatgaa
     1141 gccgtgctcg gagagcgcct gccagagctc gtgtggggac tcgtccttgg cgacgacctc
     1201 ggcgaagtaa cggtgaccga agctattggc gatgttgctg accgactcgc ggatcagctt
     1261 gtgctcgtcg gtctcgacca agggcaggga cgcgctggtg gcaagctcgc tcacctggcc
     1321 aaagtacttc ctggttggcg gctaaatagc tgtcgtgagt gctagtaggg cggccgcctg
     1381 agcggactta tgctggcgcc gatgagcgca gagctggccg gcaagagcgg catagtcacg
     1441 ggcggcgcgc gcgggatcgg cgcggcgatc gcagctgcct tcgccgaggc cggcgcgcgg
     1501 gtcgcggtct tcgaccagga cggcgacggc gccgatgcga ccgccgccga gatcggcggc
     1561 ggcgcgattt cccgcgaggt cgacgtcacc gacgaggacg tggtcgccgc cgccgtcaac
     1621 gacgtcttcg aggcctttgg cagcatcgac ttcctcgtca acaacgccgg cgtgcgccac
     1681 gtggcgccgg tcgccgagca cccggtggac gtctggcggc ggacgatcga gatcaacgtc
     1741 gtcggcacct tcatctgcag ccaggcggtg atccgggtcg cgcgcgagca cggcggctgc
     1801 cggatcctca acctcggctc gatggccggc gagctggcgc tcggcgaacg ctccgcctac
     1861 aacgccagca agggcgcggt cgaggcgctg accaaatcga tcgcggtcga tcacggcgaa
     1921 gagggcattt actgcaactc gatcgccccc ggcgtgatcg agaccccgct cagcgctccc
     1981 tacttcgaag acgaggcgat ggtcaaggtg ctgcgcgaga actcgccgat cggccgctgg
     2041 ggtcaggtcg ccgaggtcgc cgcgccggcc gtcttcctct gcagcgacgc cgccagcttc
     2101 atccagggcg agaccctgtt cgtcgacggc ggctggaccg cggggaaggg ctactgatgc
     2161 tcgactccgg gctcgagggc cgcgtctgcc tggtcaccgg tgccgccagc ggcatcggcc
     2221 tggcgagcgc ccgcatcctc gccggcgagg gcgccaagct ggcgctctgg gacctcgacg
     2281 agggcgcgct cgagggcgcc gacctcgacg acggcggctc cgccacggtc agccgcagcg
     2341 ccgtcgatat cgccgatccg gacgcggtca gggccggggt cgaggcgatc gtcgccgccc
     2401 acggcaccgt cgacgcgctg atccactgcg ccggcatcta caccaccgcg ccggtcccgg
     2461 agatcaccag cgaggactgg aaccgggtgc tcgacgtcaa cctgcgcggc gccttcaacg
     2521 tcagccaggc ggtcgccgag ccgatgcgcg agcacggcga cggccgcatc gtcctgttcg
     2581 gctccttcgc agcccgcacc ggcgggctgc gcgcgagcgc cccctacgcc gcctcgaaag
     2641 gtgggatcgc cggtttgacc aagcacctcg cgcagtgggg cggcccgctc gggatccgcg
     2701 tcaactgcat ccacccgggc ttcatccaga ccccgatgaa cgcgatcctc agcgacgagg
     2761 ccaagcagca ggcggtcgac aacacgccgc tgcgccggct cgccgacccg cccgagtgcg
     2821 cggcgatggc ggtcgtgctc gcctccgacc tggcgagctt cgtccacggc gtcgagctcg
     2881 acgtcaacgg cgggatgtac atggcatgag catcgactgg atgggaaacc gcagcctcac
     2941 cgacctcctg caggagcggc tggcgctgag ccccgacgac accttcctgg tcttcgagaa
     3001 ccgcgcgggc gaggtcagcg agctgaccta cgccgagttc gagacccggg tcgaggacgc
     3061 cgcgcgcggc ctcaacgcgc tcggggtcga ggccggcggc cgcgtcgtcg tccacctgcg
     3121 caacagcccg gagttcctga tctcgtggtt cgcgatcgtc cgcctcgggg cgacgatggt
     3181 gccctcgaac atcgccaaca cggcgggtga gctcgaacac gtcgtcggct tcaccgaggc
     3241 cagccacgcg atcaccgagc ccgacttctg cgacgcggtc cagcgcggga tcgagctggc
     3301 cgggaccgac acgacgacga tcgtcgcccg cggcaagctc gccggcttct tcgccttcga
     3361 cgacctcgcg acactgtcca gcgagcccgc cccgcggccg ccggtcagcg gcgacgacgt
     3421 cgccgagctg ctcttcacct ctggcaccac ctcgaagccg aaggcggtga tgctgaccca
     3481 cgccaactgc ctgcgcgccg ggctcgacac cgtgcactgc ctctggctcg accgcggcga
     3541 gcggatgctg accgcgctgc ccctgttcca cgtcaacggc caggggatga cgatcctggc
     3601 ggcgatgacc gtgggcggga ccgcgatcgt gatcgaggag ttcagcgcct cgaagttctg
     3661 gaagcaggtg aagcgccaca aggcgaccca gaccgcggtc gtggcgatgc agctgcgcgt
     3721 gctgatcgcc cagcccgacg accccagcga ccgcgaacac gacgtgcgca agctcttcta
     3781 cgcgatcaac gtctccgacc aggagaaaga agcgttcgag gagcggttcg ggatcgaggt
     3841 gatcaacggc tacgggctga gcgaggcgat gaccctctgc acggtctcgc cggtggcggc
     3901 gccgcggcgc tggccctcga tcggcctgcc ctcacccggc cgcaccctgc tgctgctcga
     3961 cgaagacggc aacgaggtgc cgaccggcga ggtcggggag atctgcgtca agggcatccc
     4021 cggccgctcg atcatgctcg gctactacaa ggacgagcag tcgaccgccc aggcgatccg
     4081 cggcgacctc ctgcacaccg gcgacaacgc ctacgccgac gagcagggct acctctactt
     4141 cttcgaccgc aagaaggaca tgatcaagcg cgccggcgag aacgtctccg cgatcgaggt
     4201 cgagtcggtg ctcaacgacc acccgcagat cgccgaggcc gcgatcgtcg gcatgcccga
     4261 cccgatccgc gacgaagccg tcgccgccgt gatcgtcgtc gccgagggcg ccgagctgag
     4321 cgaagaggcc gtcgtcgcct actgctccga gcgcctctcc cgcttcaagg tcccgacgat
     4381 catcgccttc gaggccgaac tgccgaaaac ctcgatcggc aaggtccgca aggacgagct
     4441 gcgcaagaag ctggcttagt tcggccggag aatccgcgct cgcgacattg aggcccctat
     4501 agggcacctg atgtagcgag ggcggtggag gctcccccct tagggggctt cgaggccgcg
     4561 gagggcgatg gcggtgaaga cctcggcgac ttcgtcggcg ctgagggggc cgtcttgctg
     4621 gaaccaggtg tagccccagt tggccatgcc gagcagggcc ttggtgacga tctcgggggg
     4681 gaggtcggcg aaggcgccgg aggagcggcc gtcgctgatg atctggctga ccatgcgctc
     4741 gtagaagtcg cgctttatga ccagggcctg ccagcgctcg ccggtcacgg cgtggcgctc
     4801 ggagaggaag acggtgacgc tctcgcggtt gcgggcgatg aagcgcatcg tcacgtggag
     4861 caggccgcgg accttctcgg cgggcgactc agaggaagag gagacggcga tcgtctcgga
     4921 gatcagctcg tcgatcaggg tctcgtggat cgcaaagagg aggtcctcct ttttgcgcac
     4981 gtggtggtag aggctggcct tgcgaatccc gaccgcgtcg gcgatttcgg ccatcgaggc
     5041 ggcgtggtag ccacgctcgc tgaagatgcg ggtcgccgct tcgatgatgt cggcgcgaac
     5101 cgtcacggtc ggcgccgatc ctggatcggt tttggtctct tgcatgctcg gcacagtctg
     5161 ctgtaacggt gcggactcta aagcacggtt atcgaccgtg cggttgcccg tccaggcgca
     5221 actcgcaccg tggaggggtt gcgcgtcccc caatgtttgt gtaaggtgcc taccggccgg
     5281 taggtagaga gccttccagc cgcatgggaa tgaccaccag ggacgagaca accgacgctg
     5341 agcgcctggc gcgataccgc ctgatgcggc tcagccgccg cttcgaggag acggtccacg
     5401 agcagttcca cgacggcaac gtccccggcc ccctgcacct ctcgatcggc caggaggcgg
     5461 tcgcgatcgg cggctgctgg ccgctgcgcg agaccgacgt gatcgtctcc acccaccgcg
     5521 gtcaccacca ctgcctcgcc aagggcgcca gagccgaccg gatgatggcc gagctgctcg
     5581 gccgcgccga cggctactcg cgcggtcgcg gcggctcgat gcacatcgcg atcccggaga
     5641 tcggcctgct cggcaccaac ggcatcgtcg gcggcgggct cccgatcggg accggcgccg
     5701 cgctcgggct gcagatcgac ggccgcgacg acgtcgccgt ctccttcttc ggcgagggcg
     5761 ccgccgccaa cggggtcttc ggcgaggcgc tcaacaccgc cagcctctgg aacctcccga
     5821 tcgtcttcat ctgcgagaac aaccgctacg tcgagctgac cccgcagcac gttcacgtcg
     5881 ccggcgagat ctggaagcgg gccgagggct acgacatgcc cggcgtcaag gtcgacggca
     5941 acgacgtcga cgcggtcgtc gagaccgtcg cggcggcggt cgaccgcgct cgggccggcg
     6001 atggaccgac tttgatcgag gccgagacgt accgctggtt cggccactac gccggcgaca
     6061 aagccgccta ccgcgacgag gccgaggtcg aggccggccg cgccggggac ccgctggtgc
     6121 ggacccgcgc cctgctcgac ccggtcgagg ccgacctggc cgaggccgag gtcgagaaag
     6181 agatcgcggc cgcgctcgag ttcgcgctgg ccagcccgat cgccggcgag gaggtcctca
     6241 cgccgggcca ctacgcagag gaggtcagcg cgtgagcacc gccgccgtcg agacccgctc
     6301 gatgaagttc tggcagggcg tcaaccaggc cctggtcgag gagatggacc gcgacgagcg
     6361 caccttcctg gtcggcgagg acgtcgcccg ggtcggcggc ccctacggga tcacccgcgg
     6421 cctgctcgac cgctacggcc cctcgcgggt ccgcgacacc ccgatctccg agtcgctgct
     6481 ggccggcctc ggcaccggcg ccgccgcggt cgggatgcgc ccgatcgtcg agatcatgtt
     6541 cttcgacttc gcgatgctgg cgatggacca gatcgtcaac caggccgcca agtaccgcta
     6601 cttcaccggc cactcgctgc cgctgacgat ccgcacgatg tgcggcgccg gcgggccgaa
     6661 cggcgcccag cactcgcaga acttcgaggc ctggttctgc gccgtccccg ggctcaaggt
     6721 cgtgatgccc tcgaacgctg ccgacgccaa ggggatgctg aagtcctcga tccgcgacga
     6781 agacccgacc ctcttcatcg agacccaggg cctgttgacc gcgcgcgccg aggtgccgct
     6841 ggaggaggac tacctggtcc cgctcggcca ggccgcgatc ccccgcgagg gcagcgacgt
     6901 caccgtggtc gcgatcggcc gcatggttga ccgggcgctg gaggcggccg agcagctggc
     6961 cgccgacggg atctcggtcg agg
//
LOCUS       DASRIG010000297         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_678426, whole genome shotgun sequence.
ACCESSION   DASRIG010000297 DASRIG010000000
VERSION     DASRIG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678426"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFB52_08110"
     CDS             complement(<1..389)
                     /locus_tag="VFB52_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06336.1"
                     /translation="MKLHRRVARHPVSRHTLNLGLRVFAILLVLAALAVPAYAYWEST
                     RPPGVELGEQIDVGLPEQPYTLPGGMPSYSDGVIALCYHDISRTADDAYAITPRAFAD
                     QMAALKAAGFETVTLDEFNAYMKGEEV"
     gene            complement(398..532)
                     /locus_tag="VFB52_08115"
     CDS             complement(398..532)
                     /locus_tag="VFB52_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06337.1"
                     /translation="MKVEPVRPQRSRRRRVADLVVSIAMLVVLVALYLVVVSRGNLPF
                     "
     gene            complement(532..1956)
                     /locus_tag="VFB52_08120"
     CDS             complement(532..1956)
                     /locus_tag="VFB52_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06338.1"
                     /translation="MSRAATALKRAPAAPAPEQEREHQPLVSASTKHWLKIGIVLACL
                     APVTVLIGLRVSKVSDEAVLTFYGLIVLLITTKVMFVAFTFYRDPAHRGGEVVSIKAP
                     LVSCLVACKDDREVIARCVLSLLDQTYGNKELIVIDDGSTDGSYEILQELEREHDDAF
                     TLLRNEEAQGKKKALVRGLEGAKGEYLVFTDSDCVIEEDAVEKMVQAFQRDPGLGAVS
                     GDARALNADHNVLTRIQDTWYDGQFSIWKATESVFGSVSCVSGPLAGFRREAIWNYFP
                     AWAADTFFGREFRFATDRQLTGYVLGQEWKGEKLKAEHADSPFVKEHYPEQRWRIGYV
                     KSAKVWTNVPPTMRSLMRQQTRWKKSFIRNLAFSAGFYWRRGLAAAVLFYAHALLVIA
                     TPFMAFRHLVMLPLEGAWVLTALFVGGVALKGGIWAVAYKIQNPDCGRWIYRPLMSLM
                     STFLFSGLLVYSLVTIRRQIWVRG"
     gene            complement(1953..>2970)
                     /locus_tag="VFB52_08125"
     CDS             complement(1953..>2970)
                     /locus_tag="VFB52_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZO06339.1"
                     /translation="AILICVPTPVDEHRNPDLTALRAACEAVCRHARRGQTIVLTSTS
                     YVGTTRDLLAQPLSEAGFELGTDICVAFSPERIDPANTAHPQETVPRVLGANSEVCAE
                     RAKAVIGAIAPTVHVVSSPEAAEMTKLYENVFRAVNISLANEMAEAAGALGLSPAEIV
                     DAAATKPYGFMPFQPGPGVGGHCIPCDPHYLLWQMRPRGQAMPLVSEAMDGIIQRPQR
                     VIERAAELLSTEAGRGLRGSRVLLLGVAYKPGVADVRESPALTLIDLLGERGAEVEYH
                     DPMVPVLDCGGETLVTVPDPKVRAYDLVIAHTLHPRADYGFLDGSTPVLDCTYRLDGR
                     KSDL"
BASE COUNT          464 a          986 c         1071 g          449 t
ORIGIN      
        1 tcgacctctt cgcctttcat gtaggcgttg aactcgtcga gcgtgaccgt ctcgaacccg
       61 gccgccttca gcgccgccat ctggtcggca aaggctcgcg gggtgatcgc gtaggcgtcg
      121 tcggccgtac gcgagatgtc gtggtaacag agcgcgatca caccgtcgct gtagctcggc
      181 atcccgccgg gcagggtgta cggctgctcg ggcagaccca cgtcgatctg ttcgccgagc
      241 tcgacgcctg gcggccgggt cgactcccag taggcgtagg cgggcacggc gagagccgcc
      301 aggaccagca ggatcgcgaa gacgcgcagg ccgaggttga gcgtgtggcg gctgacgggg
      361 tggcgggcga cgcggcggtg cagcttcatc gcttccccta gaacggcagg ttgccgcgcg
      421 agacgaccac caggtagagg gcgacgagca cgaccagcat cgcaatcgag acgacgaggt
      481 cggcgacgcg ccggcggcgg ctgcgctgag gacggaccgg ctcgaccttc atcagccacg
      541 cacccagatc tgacggcgga tcgtgaccag cgagtagacc agcagccccg agaacaagaa
      601 ggtcgacatc aggctcatca gcggccggta gatccagcgc ccgcagtcgg ggttctggat
      661 tttgtaggcg accgcccaga tcccgccctt cagcgccacc ccgccgacga acagcgcggt
      721 cagcacccag gcgccctcca acggcagcat caccaggtgg cggaaggcca tgaacggggt
      781 cgcgatcacc agcagcgcgt gcgcgtagaa gagcaccgcg gcggcgagcc cgcggcgcca
      841 gtagaagccg gcggagaagg cgaggttgcg gatgaagctc tttttccagc gcgtctgctg
      901 gcgcatcagc gagcgcatcg tcggcggcac gttggtccag accttggccg acttcacgta
      961 gccgatccgc cagcgctgct ccgggtagtg ctccttgacg aagggcgagt cggcgtgttc
     1021 ggccttcagc ttctccccct tccactcctg gccgagcacg tagccggtca gctggcggtc
     1081 ggtggcgaag cggaattcgc ggccgaagaa cgtgtcggcg gcccaggccg ggaagtagtt
     1141 ccagatcgct tcgcggcgga agccggcaag cggcccggag acgcaggaga ccgatccgaa
     1201 caccgactcg gtcgccttcc agatcgagaa ctgcccgtcg taccaggtgt cctggatccg
     1261 ggtgagcacg ttgtggtcgg cgttgagcgc gcgggcgtcg ccggaaacgg cgccgagccc
     1321 ggggtcgcgc tgaaaggcct ggaccatttt ctcgaccgcg tcctcttcga tcacgcagtc
     1381 ggagtcggtg aagaccaggt attcgccctt cgccccttcg agcccgcgca ccagcgcttt
     1441 cttcttgccc tgcgcctcct cgttgcgcag cagcgtgaag gcgtcgtcgt gctcccgctc
     1501 gagttcctgg aggatctcgt aggagccgtc ggtggagccg tcgtcgatca cgatcagctc
     1561 cttgttgccg taggtctggt cgagcagcga gagcacgcag cgggcgatca cctcgcggtc
     1621 gtctttgcag gcgaccaggc aggagaccag cggcgccttg atcgagacca cctcgccgcc
     1681 acggtgggcg gggtcgcggt agaaggtgaa ggcgacgaac atcaccttgg tcgtgatcag
     1741 caagacgatc agcccgtaga aggtgagcac cgcctcgtcg gagaccttcg agacgcgcag
     1801 cccgatcagc accgtcaccg gcgccaggca ggcgagcacg atcccgatct tcagccagtg
     1861 cttggtcgag gcggagacga gcggctggtg ctcgcgctcc tgctcgggcg cgggcgccgc
     1921 gggggcgcgc ttcagtgcgg tcgccgcgcg gctcacagat cgctcttgcg gccgtcgagg
     1981 cggtaggtgc agtcgaggac cggggtcgag ccgtcgagga agccgtagtc ggctcgcggg
     2041 tgcagcgtgt gcgcgatcac caggtcgtag gcgcgcacct tcgggtcggg aacggtgacc
     2101 agcgtctcgc cgccgcaatc gagcaccggc accatcgggt cgtggtactc gacctcggcg
     2161 ccgcgctcgc cgagcaggtc gatcagggtc agcgccggcg actcgcgcac gtcggcgacc
     2221 ccgggcttgt aggcgacccc gagcagcagc acccgtgacc cgcgcaggcc gcgaccagcc
     2281 tcggtggaga gcagctcggc ggcccgctcg atcacccgct gggggcgctg gatgatcccg
     2341 tccatcgcct cgctgaccag cggcattgcc tggccgcgcg ggcgcatctg ccagagcagg
     2401 tagtgcgggt cgcaggggat gcagtggccg ccgaccccgg ggccgggctg gaagggcatg
     2461 aagccgtagg gcttggtggc cgccgcgtcg acgatctcgg ccggcgagag gcccagcgcc
     2521 ccggccgcct cggccatctc gttggcgagc gagatgttga cggcgcggaa gacgttctcg
     2581 tagagcttgg tcatctccgc cgcctcgggc gaggagacaa cgtgcacggt cggcgcgatc
     2641 gccccgatca cggccttcgc ccgttcggcg cacacctcgc tgttggcccc gagcacccgc
     2701 ggcaccgtct cctgcgggtg ggcggtgttg gccggatcga ttcgctccgg cgagaaggcg
     2761 acgcagatgt cggtgccgag ctcgaacccg gcctcgctca gcggctgggc cagcaggtcg
     2821 cgggtcgtgc cgacgtagct ggtcgaggtg agcacgatcg tctggccgcg gcgggcgtga
     2881 cggcagaccg cctcgcaggc cgccctcagt gccgtcaggt cggggttgcg gtgctcgtcg
     2941 accggggtcg ggacgcagat caggatcgcg
//
LOCUS       DASRIG010000298         7236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1458669, whole genome shotgun sequence.
ACCESSION   DASRIG010000298 DASRIG010000000
VERSION     DASRIG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458669"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFB52_08130"
     CDS             <1..655
                     /locus_tag="VFB52_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06340.1"
                     /translation="DPDKVHVIPHGAFDYLTRLPEEKPLPEELQGAKGPVILCFGLIR
                     PYKGTEVLLEAFRRLGETAAGGDAELWIVGNPRMDTGPLEELAAAAPGRVRFVTRFVE
                     ESEIPAIFRRADVVALPYLDAEHSGVLYTGLAFGKPLVLSAVGGFPEVAATGAARLVP
                     PGDAEALAATLAELAGDEAARAELGAAATRAAAGPYSWDEAARLTLDLYRELQSRSA"
     gene            669..1667
                     /gene="purM"
                     /locus_tag="VFB52_08135"
     CDS             669..1667
                     /gene="purM"
                     /locus_tag="VFB52_08135"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZO06341.1"
                     /translation="MSDAYAKAGVDQGAADSAVAGLVRALGAIKLDRPSAQVPLAGHY
                     ASVIKIDERTGIALSTDGVGTKLVIAEEMNRFDTVGIDCVAMNVNDVICVGAEPLAML
                     DYIAIERAEPAVCEEIGVGLARGAELAGIEIPGGELAQLGEMVNGVDVSGACFGTVAL
                     DAIIDGSAVEPGDAILGLPSSGLHSNGYTLARTALAGLALEDDQLGRPLGEVLLEPTE
                     IYVKPVVELLRSPIEVRGLAHITSGGTGNLLRLAAEVGYEIDAPLPVPPVFGLIQERG
                     EVSDAEMREVFNMGCGFCVVVPAADEDAALELLRAHYPAAQRIGRAVAGAGVTQAG"
     gene            1775..3262
                     /locus_tag="VFB52_08140"
     CDS             1775..3262
                     /locus_tag="VFB52_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO06342.1"
                     /translation="MIGTVLSGRYRLEAKLGSGGMSTVYLARDQTLDRDVAVKVMHRE
                     MSEQADQLERFRQEARAVAKLSHPNVVAVIDAGEDGGHPYIVFEYVEGETLKARINRV
                     GALDPQEALAYAIEIARGLTVAHARNMVHRDIKPQNVLIDAEGRAKLTDFGISRQLEQ
                     DGMTATGRVLGTTDYVAPEQAMGMAVDPRSDIYSLGVVLYEMLIGQVPFHADSQVGVA
                     MKHVNEELPDVQQRRPEVSAAAALVVERSTAKDPDERYQEVGEMIDDLSTALEVEAAR
                     AGSSTGEATSVLQAVPPAKRKLNRARWSWAAILLIVVIGGGAVAAVTLISSGDSPGGG
                     GGALKDKGQPIELESATDFDPQGDGEEDPEVVSLAVDGSLKTAWTSEHYDDESFAGTK
                     TGPNEGVGLYVTADAPVKPSEIVIHGAPGYDAQIYAIAGEAPEDLAGWGEPVGEVTDA
                     PDVAEVPIDVTAPAKNFLIWFNRAAPSRDQDGRYQLEISDVKLIE"
     gene            3364..5799
                     /locus_tag="VFB52_08145"
     CDS             3364..5799
                     /locus_tag="VFB52_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO06343.1"
                     /translation="MRTFHQARRAPPDVLLAWGLLVGAIALYAIGLALLYRAEQPQDS
                     LSDPANLLRLAIYPALVGAIWVFLRRADGIPRTGAIVLDAAIFSLALGAVVFEGLFNW
                     FAPIGSVPEARASLLAFPLLDLVALVLLSLIAGPARSRSAVAYGILAASLLLLGLADV
                     SLAVAPDAQADTGAAGAVAWTLGVLGIGIALVAGISERGPKALEGLWLYAITTVAVTT
                     SLGLLLVESLRDREPAILVITSLVLCLALIRFALLARENGRLANDSERIIEAAGEGIY
                     RLDLEGRLTHVNPAALRLLGYDAEDDVLGKQAHSLIHHTRPNGTPYPAAECPVFRSLR
                     RGSTQRSRGEVYWRADGSSFPVEYTSAPIREGGRTVGAVVVFDDVSKQRQLEERVRLQ
                     AIHDSLTGLHNRSHLEREVLAQSRFARRYASTGAVLLIDLDAFELVNDALGHAAGDRV
                     LSDVATALVEAVGPGVLVARVDGDEFGVLLRESSLEVAVETGKRLIAAIRDRVEPAIG
                     ASVGLAVLDGSGSDGDVLVDAGVALRKAKESGRGSVVVGLGGRSPQVTLVESLREAIA
                     AGRLVVYSQPIFDLRKDEVAYEELLVRMVDASGRVVPPDSFLPTAERFGLIQQVDYFV
                     VCEAIELAREGRAVAVNLSGRSLGDARIVARVEAAVRDGLDGRRLAFEITETSAVANM
                     ADARQFAARVRELGCTVALDDFGTGFSSFAYLKNIPAQLLKIDMDFIRELPRSEPDQH
                     LVSAIVAVAESLGLRTVAEGVEDAETLAMVRELGIDYAQGHHLGRAQPVLTGMISYST
                     AARAARILADQNS"
     gene            complement(5787..6752)
                     /locus_tag="VFB52_08150"
     CDS             complement(5787..6752)
                     /locus_tag="VFB52_08150"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZO06344.1"
                     /translation="MRVAVLAGGRSSEHDVSLRSGAAVAAGLREGGHEVIEVTIARNG
                     HWGHGAGNVELVPGAGLLGAEVVFPVLHGPFGEDGSVQGLLEWLDVPYVGSDVLSSAI
                     CMDKLTLKRLFAAHGVPQVDFTAVEGNDWWGRCEQLGLPLWVKPSRLGSSVGITRVEK
                     LEELDEAVELALRHDPRVIVEASARGREVECSVLGNEQPLTSQPGEIVAHAEWYDFEA
                     KYTEGGMELRVPAPLEDETLARVRELAAEIFALAGCSGLARCDFFVEPDGEVLVNEIN
                     TMPGFTETSVYAKLLEASGIAYPELCDRLVELAVERHRHARSHEF"
     gene            complement(6777..>7236)
                     /locus_tag="VFB52_08155"
     CDS             complement(6777..>7236)
                     /locus_tag="VFB52_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZO06345.1"
                     /translation="TVTINVSRGPKLVKVPVLVGAQRDVAVERIRGRGLEPSVSEEES
                     AQPAGKVLSQSPDAGEKVDPGSTVSIVVSSGEAESKVPNVIGKERREAVEAIRAAGLV
                     PVVEEEETEVPSKVGRVIDQFPAPGQELPEGSEVTLTVGKLTSFEAETEE"
BASE COUNT         1030 a         2627 c         2522 g         1057 t
ORIGIN      
        1 cgacccggac aaggtccacg tgatcccgca cggcgccttc gactacctga cccggctgcc
       61 ggaggagaag ccgctgccgg aggagctgca aggggccaag gggccggtga tcctctgctt
      121 cggcctgatc cgcccctaca agggcaccga ggtcctgctc gaggcgttcc gccggctcgg
      181 cgagactgca gccggtgggg acgccgagct ctggatcgtc ggcaacccgc gcatggacac
      241 cgggccgctg gaggagctgg cggcagccgc gccgggtcgt gtccgctttg ttacccgctt
      301 cgtcgaggag agcgagatcc cggcgatctt ccgccgcgcc gacgtcgtcg ccctgcccta
      361 tctggacgcc gagcactccg gcgtgctcta caccgggctc gccttcggca agccgctggt
      421 gctgagcgcg gtcggcggct tccccgaggt cgcggcaacc ggggcggccc gcctcgtccc
      481 ccccggcgac gccgaggcgc tcgccgcgac cctcgccgag ctggccggcg acgaggccgc
      541 tcgcgccgag ctgggcgccg ccgccacgcg cgccgccgcc ggtccctact cgtgggacga
      601 ggccgcccgc ctcaccctcg acctctaccg cgagctgcaa agccggagcg cctgaaagaa
      661 tcggcagcgt gagcgacgct tacgcgaaag ccggggtcga ccagggggcc gccgactcgg
      721 cggttgccgg gctggtccgc gccctcggtg cgatcaagct ggatcggccc tcggcccagg
      781 tgccgctggc cgggcactac gcgagcgtga tcaagatcga cgagcgcacc gggatcgcgc
      841 tctcgaccga cggggtcggg accaagctgg tgatcgccga ggagatgaac cgcttcgaca
      901 cggtcgggat cgactgcgtg gcgatgaacg tcaacgacgt gatctgcgtc ggcgccgagc
      961 cgctggccat gctcgactac atcgcgatcg agcgcgccga accggcggtc tgcgaggaaa
     1021 tcggggtcgg cctggcgcgc ggcgccgagc tggccgggat cgagatcccc ggcggcgagc
     1081 tcgcccagct cggcgagatg gtcaacggcg tcgacgtctc cggcgcctgc ttcggcacgg
     1141 tcgcgctcga cgcgatcatc gacggctccg cggtcgagcc cggcgacgca atcctcggcc
     1201 tgccctcctc cggcctgcac tcgaacggct acacgctcgc ccgcaccgcg ctcgccgggc
     1261 tggcactcga agacgaccag ctcgggcggc cgctcgggga ggtcctgctc gagccgaccg
     1321 agatctacgt gaagccggtg gtcgagctgc tgcgctcgcc gatcgaggtg cgcggcctcg
     1381 cccacatcac ctcgggcggc accggcaacc tgctgcggct ggcagccgag gtcggctacg
     1441 agatcgacgc gccgctgccg gtgcccccgg tcttcggcct gatccaggag cgcggcgaag
     1501 tcagcgacgc cgagatgcgc gaggtcttca acatgggctg cggcttctgc gtcgtcgtcc
     1561 ccgccgccga cgaggacgcc gccctggagc tgctgcgcgc ccactatccg gcggctcagc
     1621 ggatcggccg cgcggtcgcg ggcgcaggcg tcacgcaggc cggctgaggc gcagttctgc
     1681 tgccaggcgt ccgctaaatc ttcacaacgc ctgccgcgcc tcataacgtt gggctggacc
     1741 ccccggaatg gaccgcgcct cgacaagctc acagatgatc ggcaccgtgc tatcggggcg
     1801 ctaccgcctg gaggcgaagc tcggcagcgg cggcatgtcg acggtctatc tggcccgcga
     1861 ccagacgctt gaccgcgacg tcgcggtcaa ggtcatgcac cgcgagatgt ccgagcaggc
     1921 cgaccagctg gagcgcttcc gccaggaggc gcgggcggtg gcgaagctct cccacccgaa
     1981 cgtggtcgcg gtgatcgacg ccggcgagga cggcggccac ccctacatcg tcttcgagta
     2041 cgtcgaaggg gagacgctga aggcgcggat caaccgggtc ggggcgctcg acccgcagga
     2101 ggcgctcgcc tacgcgatcg agatcgcccg cgggctgacc gtcgcccacg cgcgcaacat
     2161 ggtccaccgc gacatcaagc cgcagaacgt gctgatcgac gccgagggac gggcgaagct
     2221 gaccgatttc gggatctcgc gccagctcga gcaggacggg atgaccgcga ccgggcgggt
     2281 gctcggcacc accgactacg tcgcccccga gcaggcgatg gggatggcgg tcgacccgcg
     2341 ctcggacatc tactcgctcg gcgtcgtgct ctacgagatg ctgatcgggc aggtcccctt
     2401 ccacgccgac agccaggtcg gcgtggcgat gaaacacgtc aacgaggaac tgcccgacgt
     2461 gcagcagcgc cgcccggagg tctcggcggc ggcggcgctg gtggtcgagc gctcgaccgc
     2521 gaaggacccc gacgagcgct accaggaggt cggcgagatg atcgacgacc tctcgaccgc
     2581 gctcgaagtc gaggccgcgc gagcgggctc cagcaccggc gaggcgacca gcgtgctgca
     2641 agcggtgccg ccggccaagc gcaaactcaa ccgcgcccgc tggtcctggg cggcgatcct
     2701 gctgatcgtc gtgatcggcg gcggcgcggt ggcggcggtg acgctgatct ccagcggtga
     2761 cagccccggc ggtggcggtg gggcgctgaa agacaagggc cagccgatcg agcttgaatc
     2821 ggcgaccgac ttcgaccccc agggcgacgg cgaagaggat ccggaagtcg tcagcctcgc
     2881 ggtcgacggc agcctcaaga ccgcctggac cagtgagcac tacgacgacg aaagcttcgc
     2941 cgggacgaag accggcccga acgaaggcgt cggcctctac gtgaccgccg acgcgccggt
     3001 gaaaccgagc gagatcgtga tccacggcgc cccgggctac gacgcccaga tctacgcgat
     3061 cgccggcgaa gcgccggagg acctggcggg ctggggcgaa ccggtgggcg aggtgaccga
     3121 cgcgcccgac gtcgccgagg tcccgatcga cgtcaccgcg ccggcgaaga acttcttgat
     3181 ctggttcaac cgcgccgccc cctcgcgcga ccaggacggc cgctatcagc ttgagatcag
     3241 cgacgtgaag ctgatcgagt gaagttgccc ggtcgcctct agggacgcgc acggtatacc
     3301 gcgtcccgcg gaggtggatt gccctctgac aaatggggaa cttgagcgtt ctgacactaa
     3361 atggtgcgca cgttccatca ggctcgtcgt gctccaccgg atgtcctcct cgcctggggg
     3421 ctgctggtcg gagcgatcgc tctttacgcg atcggcctgg cgctgctcta ccgggccgag
     3481 cagccccagg actcgctcag tgaccccgcc aacctgctgc gactggcgat ctaccccgcc
     3541 ctggtcggcg ccatctgggt gttcctccgc cgtgccgacg ggatcccgcg gaccggcgcg
     3601 atcgtgctcg acgcggcgat cttctcgctg gcgctcggcg ccgtcgtctt cgagggcctg
     3661 ttcaactggt tcgcgccgat cggctcggtg cccgaggcac gcgcctcact gctcgccttc
     3721 cccctgctcg acctcgtcgc gctggttctg ctcagtctga tcgccggccc cgcgcgcagc
     3781 cgctcggccg ttgcctacgg catcctcgcc gcgtccctgc tgctgctcgg cctcgccgac
     3841 gtctcgctgg ccgtggcacc ggacgcgcag gccgataccg gtgcggccgg cgcggtcgcc
     3901 tggacgctcg gcgtgctcgg catcggcatc gccctggtcg ccggcatctc cgagcgtggg
     3961 ccgaaggccc tcgaaggcct ttggctgtac gcgatcacca ccgtcgcggt caccacctct
     4021 ttgggactgc tgctggtcga gtccctgcgc gaccgcgaac ccgcaattct cgtgatcacc
     4081 agccttgtgc tctgcctggc gctgatccgc ttcgccctgc tggcgcgtga gaacgggcgg
     4141 ctcgcaaacg acagcgagcg gatcatcgag gcggccggcg aggggatcta ccggctcgac
     4201 ctcgaggggc ggctgaccca cgtcaacccg gcggcgctgc gcttgctcgg ctacgacgcg
     4261 gaggacgacg tgctcggcaa gcaggcgcac tcgctgatcc accacacccg ccccaacggc
     4321 accccgtacc ccgccgccga gtgcccggtc ttccgctcgt tgcgccgcgg ctcgacccag
     4381 cgcagccgcg gcgaggtcta ctggcgcgcc gacggcagca gcttcccggt cgagtacacc
     4441 agcgcgccga tccgcgaggg cgggcgcacg gtcggcgccg tggtcgtctt cgacgacgtc
     4501 tccaagcagc gccagctcga ggagagggtg cggctgcagg cgatccacga ctcgctcacc
     4561 ggcctgcaca accgcagcca cctggagcgc gaggtgctgg cgcaatctcg cttcgcgcgc
     4621 cgctacgcca gcaccggtgc ggtcctgctg atcgacctcg acgccttcga gctggtcaac
     4681 gacgcgctcg gccatgcggc cggggaccgc gtgctctccg acgtcgccac ggcgctggtc
     4741 gaggcggtcg gtccgggagt gctggtcgcc cgcgtcgacg gcgacgagtt cggggtgctg
     4801 ctgcgcgagt cgagcctcga ggtcgcggtt gagacaggca aacgcctgat cgcggcgatc
     4861 cgcgaccggg tcgagccggc gatcggggcc agcgtcgggc tcgcggtgct cgacggcagc
     4921 ggttccgacg gcgacgtcct ggtcgacgcc ggcgtcgccc tgcgcaaagc gaaggagtcc
     4981 gggcgcggct ccgtcgtcgt cggcctcggc gggcgctcgc cccaggtgac gctggtcgag
     5041 agcctgcgcg aagcgatcgc ggcggggcgc ctggtcgtct actcgcagcc gatcttcgac
     5101 ctgcgcaagg acgaggtcgc ctacgaggag ctgctggtgc ggatggtcga cgcgagcggg
     5161 cgcgtggtcc caccggactc tttcctgccg acggccgagc gcttcgggct gatccagcag
     5221 gtcgactact tcgtcgtctg cgaggcgatc gaactggccc gcgaggggcg cgcagtcgcg
     5281 gtcaacctct ccggccgctc gctcggcgac gcccgcatcg tcgcccgggt cgaggccgcg
     5341 gtccgcgacg gcctcgacgg caggcgcctc gccttcgaga tcaccgagac ctcggcggtc
     5401 gccaacatgg ccgacgcccg ccagttcgcg gcccgcgtgc gggagctcgg ctgcacggtc
     5461 gcgctcgacg acttcggcac cggcttcagc tccttcgcct acctcaaaaa catccccgcc
     5521 cagctgctca agatcgacat ggacttcatc cgcgagctgc cccgctccga gcccgatcag
     5581 cacctggtct cggcgatcgt cgccgtcgcc gagagcctcg gcctgcggac ggtcgccgag
     5641 ggggtcgagg acgccgagac cctggcgatg gtccgcgagc tcggcatcga ctacgcccag
     5701 ggacaccatc tcggccgcgc ccagccggtc ctgaccggga tgatcagcta ctcgaccgcc
     5761 gcccgcgcgg cgcgcatcct cgccgatcag aactcgtgag agcgggcgtg gcggtggcgt
     5821 tcgaccgcca gctcgaccag gcggtcgcag agctcggggt aggcgatccc gctcgcctcg
     5881 agcagcttcg cgtagacgct ggtctcggtg aagccgggca tcgtgttgat ctcgttgacc
     5941 agcacctcgc cgtcgggctc gacgaagaag tcgcagcggg cgaggccgga gcagccggcc
     6001 agggcgaaga tctccgccgc cagctcgcgc acgcgcgcca gggtctcgtc ctccagcggg
     6061 gcgggcacgc gcagctccat cccgccctcg gtgtacttgg cctcgaagtc gtaccactcg
     6121 gcgtgggcga cgatctcgcc cggctgggag gtgagcggct gctcgttgcc gagcaccgag
     6181 cactcgacct cgcggccgcg cgccgaggcc tcgacgatca cccgtgggtc gtggcgcagg
     6241 gccagctcga ccgcctcgtc gagctcctcc agcttctcga ccctggtgat gccgacgctg
     6301 gagccgagcc gcgagggctt cacccagagc ggcagcccga gctgctcgca gcgaccccac
     6361 caatcgttgc cctcgaccgc ggtgaagtcg acctggggca cgccgtgggc ggcaaacagg
     6421 cgcttcaggg tcagcttgtc catgcagatc gccgaggaga gcacgtcaga gccgacgtag
     6481 ggcacgtcga gccactcgag cagcccttgc acgctgccgt cctcgccgaa gggcccgtgc
     6541 aggaccggga agacgacctc ggcgccgagc agcccggcgc cggggacgag ctcgacgttg
     6601 ccggcgccgt ggccccagtg gccgttgcgg gcgatcgtca cctcgatgac ctcgtggccg
     6661 ccctcgcgca ggccggcggc gacggcggcg cctgagcgca gcgagacgtc gtgctccgag
     6721 gagcggccgc ccgcgagcac cgccaccctc atccgcgcgt cctttcgctc acgcgctcat
     6781 tcttccgttt ctgcttcgaa ggaggtcagc ttgccgaccg tgagcgtcac ttccgagccc
     6841 tccgggagtt cctggccggg cgccgggaac tggtcgatca cccgcccgac tttgctcggg
     6901 acttcggttt cttcttcttc gaccaccggc accaggcccg cggcgcggat cgcttccacc
     6961 gcttcgcggc gttctttgcc gatcacgttc gggactttgc tttccgcttc gccgctggag
     7021 acgacgatcg agacggtcga gccgggatcg accttttcgc cggcgtcggg cgactgcgag
     7081 agcaccttgc ccgcgggctg cgcgctttct tcttcggaga ccgacggttc gagcccgcgg
     7141 ccgcggatcc gttcgaccgc gacgtcgcgc tgggcgccga ccaggaccgg caccttgacc
     7201 agcttcggcc cgcgcgagac gttgatcgtc acggtc
//
LOCUS       DASRIG010000299         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_930405, whole genome shotgun sequence.
ACCESSION   DASRIG010000299 DASRIG010000000
VERSION     DASRIG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930405"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VFB52_08160"
     CDS             <1..1509
                     /locus_tag="VFB52_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO06346.1"
                     /translation="DEGLDRSTWIVAGVVLLGAVMSILDTTVINVAIDRLAIDFNSSL
                     TTIQWVVTGYTLALAAVIPVTGWAADRFGTKRIYIWSLILFIAGSILCAAAWSAGSLI
                     AFRVLQGIGGGMIMPAVMTIMTRKAGPHRMGRVMGILGVPMLVAPLVGPILGGWLVDD
                     VSWRWIFLINVPIGIVAVALAMIVLERDEPQPAHKLDWLGMALLSPGLTALIFGLAES
                     SGDGFGATKSWLPIAAGVALIAGFFWHSWRAEAPLIDIKTFTHTRAGASAVVFMAFSI
                     AFFGALLLIPLYYQTVRGASALEAGLLLAPQGLGAMITMPLAGRITDRHGPSLLPACG
                     VPLLVIGLAPFAFVTGTTSYVLLCGFGFVLGLGMGLSMMPTMTAAMQAVPASAIARTS
                     TAMNIIRQSGASIGTALLSVLLATAIANKLPSGTSPEGGSGFEALHGISDAKREVVAG
                     PLAEAFASTFVWALALLALAFIPALWMALASRKKPADQSAAPSPQEPHPVLE"
     gene            1543..>2625
                     /locus_tag="VFB52_08165"
     CDS             1543..>2625
                     /locus_tag="VFB52_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06347.1"
                     /translation="MAKWGVLAVLGAAQFLMVLDQAVMNVAISQLVEDFDTTVTTIQA
                     VIALYALVMAGLMLTGGKLGDLFGRRRAFAIGLVIYGAGSALTAASWSVPSLTFGWSI
                     LEGIGAALVLPAMFALVAGNFQGSDRALAYGVLGGVAGAGIAVGPILGGYATTELTWR
                     VVFVGEVVVAIGILIGLRLIKEPERPATRPSMDWVGAVLSALGLGLFVFGVIQASNWG
                     WLQPKDSPIEPFGFSLTPFVVAAGALVLGAFRAWERRRERVGAEPLVHLDLLSIPRLR
                     GCVSMLLAQNLVLMGIFFTVPLFLQIVQGLDALETGVRMLPASVGLFVAALGGSALAK
                     RFPAKALVLPAMFALVAGNFQGSDRAL"
BASE COUNT          318 a          954 c          876 g          477 t
ORIGIN      
        1 gacgaaggtc tcgaccgctc gacctggatc gtcgccggcg tcgtcctcct cggtgcggtg
       61 atgtcgatcc tcgacaccac cgtgatcaac gtcgcgatcg accgcctggc gatcgacttc
      121 aacagctcgc tgaccacgat ccagtgggtg gtcaccggct acacgctggc gctcgccgcg
      181 gtgatcccgg tcaccggctg ggccgccgac cgcttcggca ccaagcggat ctacatctgg
      241 tcgctgatcc tcttcatcgc cggctcgatc ctctgcgccg ccgcctggag cgccggctcg
      301 ctgatcgcct tccgcgtcct ccaggggatc ggcggcggca tgatcatgcc cgcggtgatg
      361 acgatcatga cccgcaaagc ggggccccat cggatgggac gcgtgatggg catcctcggc
      421 gtgccgatgc tggtcgcgcc gctggtcggc ccgatcctcg gcggttggct ggtcgacgac
      481 gtctcctggc gctggatctt cttgatcaac gtgccgatcg ggatcgtcgc ggtcgcgctg
      541 gcgatgatcg tgctcgagcg cgacgagccg caacccgccc acaagctcga ttggcttggg
      601 atggcgctgc tctcaccggg cctgacggcg ctgatcttcg gcctcgccga gtcctccggc
      661 gacggcttcg gggcgaccaa atcgtggctg ccgatcgccg ccggcgttgc tctcatcgcc
      721 gggttcttct ggcacagctg gcgggcggag gcgccgctga tcgacatcaa gaccttcacc
      781 cacacccggg cgggagcctc ggccgtggtc ttcatggcct tctcgatcgc cttcttcggc
      841 gccctcctgc tgatcccgct ctactaccag acggtgcgcg gcgcatcggc tctggaagcg
      901 gggttgctgc tggctccgca gggtctcggc gcgatgatca cgatgccgct ggcggggcgg
      961 atcaccgacc gccacgggcc cagcttgctg cccgcctgcg gcgtcccgct cctggtgatc
     1021 gggctcgctc ccttcgcctt cgtcaccggc acgacctcct acgttttgct ctgcggcttc
     1081 ggcttcgtgc ttggcctcgg catgggcctt tcgatgatgc cgacgatgac cgcggcgatg
     1141 caggcggtgc cggcaagcgc gatcgcccgc accagcacgg cgatgaacat catccgccag
     1201 tcgggcgcct cgatcggcac cgcgctcctc tcggtcctgc tcgcgaccgc gatcgcgaac
     1261 aagctgccga gcgggaccag tcccgaaggc ggcagcggct tcgaggccct gcacggcatc
     1321 tccgacgcca aacgcgaagt cgtcgccggc ccgctcgccg aagcctttgc ctcaaccttc
     1381 gtctgggcgc tggcactgtt ggcgctggcc ttcatcccgg cgctctggat ggcgctcgcc
     1441 agccgcaaaa agccggcgga ccagagcgcg gccccctccc cccaggagcc gcaccccgta
     1501 ctcgagtagg accgccctgt cgccctcgaa tagggtgcgc gtgtggcgaa atggggagtg
     1561 ctcgcagtcc taggtgcagc gcagttcctg atggtcctgg accaggcggt gatgaacgtc
     1621 gcgatctcgc agctggtcga ggacttcgac accaccgtga ccacgatcca ggcggtgatc
     1681 gcgctctacg cgctggtcat ggccgggctg atgctgaccg gcggcaagct cggagacctc
     1741 ttcggccgcc gccgcgcgtt cgcgatcggg ctggtcatct acggcgccgg ctcggccctg
     1801 accgccgcct cctggagcgt gcccagcctc accttcggct ggtcgatcct cgagggaatc
     1861 ggcgcggccc tggtcctgcc ggcgatgttc gcgctggttg ccggcaactt ccagggctca
     1921 gaccgggccc tcgcctacgg ggtgctcggc ggcgtcgccg gcgccgggat cgcggtcggc
     1981 ccgatcctcg gcggctacgc gaccaccgag ctgacctggc gggtcgtctt cgtcggcgag
     2041 gtcgtggtcg cgatcggcat cctgatcggc ctgcggctga tcaaggagcc cgagcgcccg
     2101 gctacccggc cgagcatgga ctgggtcggc gcggtgctct cggcgctcgg cctcggcctc
     2161 ttcgtcttcg gcgtgatcca ggccagcaac tggggctggt tgcagccaaa ggactccccg
     2221 atcgagccgt tcggcttctc gctgaccccg ttcgtggtcg ccgccggggc gctcgtgctc
     2281 ggcgccttcc gcgcctggga gcgacggcgc gagcgggttg gcgccgaacc gctcgtccac
     2341 ctcgacctgc tctcgatccc gcgcctgcgc ggctgcgtct cgatgctgct cgcccagaac
     2401 ctggtgctga tggggatctt cttcaccgtg ccgctcttcc tccagatcgt ccagggcctc
     2461 gacgcactgg agaccggggt gcggatgctg cccgcctcgg ttggcctctt cgtcgccgcg
     2521 ctgggcggct cggcgctggc caagcgcttc ccggccaagg ccctggtcct gccggcgatg
     2581 ttcgcgctgg ttgccggcaa cttccagggc tcagaccggg ccctc
//
LOCUS       DASRIG010000300         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1204037, whole genome shotgun sequence.
ACCESSION   DASRIG010000300 DASRIG010000000
VERSION     DASRIG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204037"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFB52_08170"
     CDS             <1..1342
                     /locus_tag="VFB52_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06348.1"
                     /translation="YALLWGRELAHGLSPDVGSALPPTPHPLTELLGLVTTPLGDGAI
                     AVTMVIAYAVLGLIGFFVYRLGAIWFDRWIGAVAAAIVLTRAPFLSNGLRAYIDLPYI
                     ALCLGALLWEAKRPRAGWPVLALLALAGLLRPEAWLFAVAYWAWLAFDLRERKLVWRR
                     ERGTEELFLLAILAIAGPVLWALFDAITAGDPLYSWTGTKETVETLERKTGPVDLVLY
                     GPRALGEVLQWPGIVGAAGGIVLGLAFLRRRCGLGVAAAFLALLAFAILACGGLAILP
                     RYTMLAAAILAIFTALGLLGWRLLPKEHPWRRWWMAFAGVVALMFVVWAPNQWDLDSQ
                     VDTDLTNQARIEDDLNDLVDDGAFEPLCGPIAVPNHRAVPRLAFGLDIRPSEVVSYSE
                     DVPPERGYFVVPADEFVIENFILDPGDPTEINVEPPAGYELVAENESWLVYRRC"
     gene            complement(1344..1889)
                     /locus_tag="VFB52_08175"
     CDS             complement(1344..1889)
                     /locus_tag="VFB52_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06349.1"
                     /translation="MRLVPASYKRELLAVGFVVVVGLVACLLASSNDAEARPGNTTSV
                     IPNGSYGAYKAARGEYVYFTVRNRKVRDLEFQIRIICQASDEPNSEERFFTAGAAAPE
                     GRTVPANGKLTMNWQEDGGGRYGRVHTELKFGARDVANFAVIVPEQRSGGPEDTYESC
                     DGVSALRFHRGFELPPGATMP"
BASE COUNT          252 a          726 c          695 g          337 t
ORIGIN      
        1 ctacgcgctc ctctggggac gcgaactggc gcacgggctg agcccggacg tcggctcggc
       61 gctgcccccc accccgcacc cgctgaccga gctgctcggc ctggtgacga cgccgctcgg
      121 cgacggcgcg atcgcggtga cgatggtgat cgcctacgcg gtcctcgggc tgatcgggtt
      181 cttcgtctac cggctcgggg cgatctggtt cgaccgttgg atcggcgccg tcgccgccgc
      241 gatcgtgctc acccgcgcgc ccttcctctc caacggcctg cgcgcctaca tcgacctccc
      301 ctacatcgcc ctctgcctcg gtgccctgct ctgggaggca aagcgcccgc gcgccggctg
      361 gccggtcctg gcgctgctgg cgctcgccgg gctgctgcgc ccggaggcct ggctgttcgc
      421 ggtcgcctac tgggcctggc tggcgttcga cctgcgagag cgcaagctgg tctggcgccg
      481 cgagcgcggc accgaagagc tcttcctgct cgccatcctc gcgatcgccg gtcccgttct
      541 ctgggcgctc ttcgacgcga tcacggccgg cgacccgctc tattcctgga ccgggaccaa
      601 agaaaccgtc gagaccctgg aacgcaagac cggcccggtc gacctcgtcc tctacgggcc
      661 gcgcgcgctc ggcgaggtcc tgcagtggcc gggcatcgtc ggggcggccg gcgggatcgt
      721 gctcgggctc gccttcctgc gccgccgctg cggcctcggc gtcgccgccg ccttcctcgc
      781 cctgctcgcc ttcgcgatcc tcgcctgcgg cggcctcgcg atcctgcccc gctacacgat
      841 gctggcggcg gcgatcctgg cgatcttcac cgcgctcggc ctgctcggct ggcgcctgct
      901 gccgaaggag cacccctggc ggcgctggtg gatggcgttc gccggagtgg tcgcgctgat
      961 gttcgtggtc tgggcgccga accagtggga cctcgactcg caggtcgaca ccgacctgac
     1021 caaccaggcg cgaatcgagg atgacctcaa cgacctcgtc gacgacggcg ccttcgagcc
     1081 gctctgcgga ccgattgcgg tgcccaacca tcgcgccgtg ccccggctcg ccttcggcct
     1141 cgacatccgg cccagcgagg tcgtcagcta cagcgaggac gtacccccgg agcgcggcta
     1201 cttcgtcgtc ccggcagacg aattcgtgat cgaaaacttc atcctcgacc cgggcgatcc
     1261 gaccgaaatc aacgtcgagc cgcccgcggg ctacgagctg gtcgccgaga acgagtcctg
     1321 gctggtctat cgccgctgct gagctagggc atcgtcgcgc cgggcggcag ctcgaagccg
     1381 cggtggaagc gcagcgcgct gaccccgtca caggactcat aggtgtcttc gggcccgccg
     1441 ctgcgctgtt cgggcacgat cacggcgaag ttggcgacat cgcgggcgcc gaatttgagt
     1501 tcggtgtgga cgcgtccgta gcggccgccc ccgtcctcct gccagttcat ggtcagtttc
     1561 ccgttggcgg ggaccgtgcg gccctctggc gccgcggcgc cggcggtgaa gaagcgctct
     1621 tcggagttcg gttcgtcgga ggcctggcag atgatccgga tctggaactc gaggtcgcgg
     1681 accttgcggt tgcggaccgt gaagtagacg tattcgccgc gcgccgcttt gtaggcgccg
     1741 tagctgccgt tcgggatcac cgaggtggtg ttccccggcc gcgcctcggc gtcgttggag
     1801 gaggcgagca ggcaggcaac caggccgacc acgacgacaa aaccgacggc gagcagttcg
     1861 cgcttgtagg aagcagggac gaggcgcatc cctccccttt tcgggtcgcg cgagcgccga
     1921 cttgagcggc gaatgccgcg gccctaagcg cgctcgaccg cgtcgatcgc cgcgtgggtc
     1981 tcggccgcga ggtcggggtc ggcgaggtcg
//
LOCUS       DASRIG010000301         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3541176, whole genome shotgun sequence.
ACCESSION   DASRIG010000301 DASRIG010000000
VERSION     DASRIG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541176"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFB52_08180"
     CDS             <1..354
                     /locus_tag="VFB52_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZO06350.1"
                     /translation="LDEQEWEFMRKHTLIGERIIASAPALVPVARLVRSSHERWDGSG
                     YPDGLHGEQIPIGSRIIGVCDAYQAMVAERPYSVAMRPGRALEELSNCAGAQFDPQIV
                     DALRRVVSAPTRASA"
     gene            complement(335..1339)
                     /locus_tag="VFB52_08185"
     CDS             complement(335..1339)
                     /locus_tag="VFB52_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZO06351.1"
                     /translation="MKGVLVRRQFVLPAIILASVWALFAGTAQANTVTVGSVLPPGAV
                     SEPVGEVQTFLNTALPEKGASLASPVSGTVVRWRLVGAKGGPFFLRILRPNGLGAYTA
                     VGTSNPVIPNTTGLETFNTNLPIKSGDLLAVDPTNKTDELGFVAAAGGAFTTIFPSPF
                     EGATVPGREPVSGKEIELSAEVQPTPEVDSITPTSGSVTGGTEVKITGTDFTGASAVK
                     FGALTAASFKVESDTEIVAKAPKSKKVGTFDVTVTTLAGTSAAVRGDRFTYNGCVVPK
                     LHNLKLGKAKQAIQKGGCKLGKVTLRRGVTKNSGKVVQQSPKPKAVLAKGTKVKLTLG
                     "
     gene            complement(1461..2315)
                     /locus_tag="VFB52_08190"
     CDS             complement(1461..2315)
                     /locus_tag="VFB52_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZO06352.1"
                     /translation="MRRHGNREELGGAAMILASASSIQLGAALATTTFDTISPVSAVG
                     VSYAFAALFLLAVQSPRVREWTREKWRDAIVLGLVAAVASSALFLALDHLPLGSAVTV
                     QFLGPLGVAIWTGRRRRGVLIAAALAMLGVALVSSATPSGDLVGIAFALVAACGWALY
                     ILAAPRVGRHSGAGEGLAVAMCVTAIVVLPFSAGAAPHLGSLEILGALAVVGIFGRAI
                     PFRLELAALTRLRPSTAGVLFSVEPAIAALVGAVTLGESMAATQYLGILAVVAAGTLV
                     LRDVPEGG"
     gene            complement(2318..3346)
                     /locus_tag="VFB52_08195"
     CDS             complement(2318..3346)
                     /locus_tag="VFB52_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06353.1"
                     /translation="MALALAWGSHPAVAVVVVIDLFLAGAVLAAVFHAEVIAERVGEP
                     YGSLVLAVAVTVIEVGLIVTLMVAGGNETETLARDTVFAAVMITCNGVIGLAILTAAM
                     KERLARFNAEGSATAFATVITLATLSLVLPTFTTSKTGPEFSASQLAFAAVASLALYV
                     LFLVVQTIRHRSHFLPEGEEDEPAHDGPPPTRAAALRSFGLLAVSLVAVVGLAKVSSG
                     AIEDGVKSIGAPQSAVGVVIALLVLMPETLAATRNAARGRTQISFNLGYGSAMASIGL
                     TIPVIALASIWLDGPLVLGLGSTQIVLLVLTALVSSLTLLPGRATLQEGGVHLILFAA
                     FLFLAFNP"
     gene            complement(3385..4653)
                     /locus_tag="VFB52_08200"
     CDS             complement(3385..4653)
                     /locus_tag="VFB52_08200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06354.1"
                     /translation="MQGPLAYSNFRRTFLARIVSGAGSWMQTVAAGWLVYEITNSAAA
                     VGVLTVVSRGPGILLSGLGGQLADRYDGRRLIILLSAIEAVAATLLAVLGWSAGTGAS
                     VLLIYLAVLVIGAAGALASAAQQTVVTASVPPELAKRATGLASVAFNISRLVGPALGG
                     VLVVWLGVGWCFALNAVSYLFVAFAVWGLSLPPRKRSQPRRLREAARIVRRDLLLRDL
                     MIGAALFSLIVAPIQELAPSIAAAHGEGAHLLGFLLAAMALGGIIGNRIRTHLEGRGA
                     GNTLLVSGALFAGALALAILGLTSAAGIGIFGSDTYDYALAIVAMVFGGIAWDLIYVV
                     GMTGIQLRSEEIAGVMIGLFLTVTITGLTVGALAMGLLFDYVGLGATLLVGGGLMAAI
                     ALRFGLGGVGERAARGAAERAIDSTRSSSA"
     gene            complement(4750..5406)
                     /locus_tag="VFB52_08205"
     CDS             complement(4750..5406)
                     /locus_tag="VFB52_08205"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO06355.1"
                     /translation="MALTIGSDAPNFTAETTEGTIDFHEWIGDDWAVLFSHPRAFTPV
                     CTTELGYMASIKGDFDKRGVKIIGLSTDPLENTEQWSDDIAETQGTAPNYPLIADTDH
                     NVSKLYEMLPADVEGDPTERTPAQNQTLRNVFVIGPDKKIKLILVYPMTTGRNFDEVL
                     RVIDSLQLTASNQVATPAQWQPGEDVIIAGSVNDEQAKERYPDGWESPKPYIRIVPAP
                     "
     gene            complement(5445..>5943)
                     /locus_tag="VFB52_08210"
     CDS             complement(5445..>5943)
                     /locus_tag="VFB52_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZO06356.1"
                     /translation="ACRSRWRWATIRTPSVARSPKFATSADPVPLLYADSSALVKLVR
                     REPETNALRADVEGTDLASSELVLTEVPRALQRIAAADPTFLLAPALDRAGELIDELA
                     LYPIDRALLLGAGALAEPSLRTLDAIHVASAVELGSVDAFVTYDERQAAAARLAGLRT
                     MSPGV"
BASE COUNT          937 a         2056 c         2112 g          838 t
ORIGIN      
        1 ctcgacgagc aggagtggga gttcatgcgc aaacacaccc tgatcggcga gcggatcatc
       61 gcctcagccc cggccctggt cccggtcgcc cgcctggtcc gctccagcca cgagcgctgg
      121 gacggcagcg gctacccgga cggcctgcac ggcgagcaga tcccgatcgg ctcgcggatc
      181 atcggcgtct gcgacgccta tcaggcgatg gtcgccgagc gcccctacag cgtcgcgatg
      241 cgccccggac gggcgctcga agagctgtcg aactgcgccg gagcgcagtt cgacccacag
      301 atagtggacg cccttcgtcg cgtcgtctcg gcccctaccc gagcgtcagc ttgaccttgg
      361 tgcccttggc gagcaccgcc ttcggcttcg gcgactgctg caccaccttg ccactgtttt
      421 tcgtcacgcc tctgcgcaag gtgaccttgc cgagcttgca gccacccttc tgaatcgcct
      481 gcttggcctt gccgagcttc aggttgtgca gcttcggcac cacgcagccg ttgtaggtga
      541 agcggtcgcc gcggacggcg gcgctggtcc cggccagggt ggtgacggtg acgtcaaacg
      601 tcccgacctt cttgctcttc ggcgctttgg cgacgatttc ggtgtcggat tcgactttga
      661 agctcgccgc cgtcagggcg ccgaatttga ccgcgctggc gccggtgaag tcggtgccgg
      721 tgatcttcac ctcggtgccg ccggtgaccg agccgctggt cggggtgatc gagtcgacct
      781 ccggggtcgg ctggacttcc gcgctgagct cgatctcttt gccggagacc ggttcccggc
      841 cgggcacggt cgcgccttcg aacggggacg ggaagatcgt cgtgaacgct cctcccgccg
      901 cggcgacgaa gccgagttcg tcggttttgt tggtcgggtc gacggcgagc aggtcgccgg
      961 acttgatcgg caggttggtg ttgaacgtct ccagcccggt cgtgttcggg atcaccgggt
     1021 tgctggtgcc gaccgcggtg taggcgccga gtccgttcgg gcgcaggatc cgcaggaaga
     1081 acggaccgcc tttggcgccg accaggcgcc agcgcacgac cgtgcccgag accggcgagg
     1141 cgaggctcgc gcctttctcc ggcagcgcgg tgttgaggaa cgtctgcact tcaccgaccg
     1201 gttcggaaac cgcgccgggc ggcagcacgc tgccgaccgt caccgtgttg gcctgtgcgg
     1261 taccggcgaa gagcgcccag acgctcgcca agatgatcgc gggcagcacg aactgccggc
     1321 gtacgagtac acctttcatg atttcccctt gctccgtgtg atcccgcacc agacggcggg
     1381 acttgtcttt gggtcccgca cagggcggcg gaaccgtttg ttctgatcgg ggaaaaccgc
     1441 gaggggcggg caaagttgcg ctagccgccc tcgggcacgt ctcgcagcac cagggtgccg
     1501 gcggcgacga cggcgaggat gccgaggtac tgggtcgcgg ccatgctctc gccgagggtg
     1561 acggcgccga ccagggcggc gatcgcgggc tcgacgctga acagcacgcc ggcggtcgac
     1621 gggcgcagcc gggtcagcgc cgccagctcg agccgaaacg ggatcgcgcg gccgaagatg
     1681 ccgaccacgg cgagcgcgcc gaggatctcg aggctgccga gatgaggagc cgccccggcg
     1741 ctgaacggca ggacgacgat cgcggtcacg cacatcgcca ccgccagccc ttcgccggcg
     1801 ccggagtggc gcccgacccg gggcgcggcg aggatgtaga gcgcccagcc gcaggcggcg
     1861 acgagcgcga aagcgatccc gaccaggtcg ccgctcgggg tggcgctgct gaccagggcg
     1921 accccgagca tcgccagcgc cgccgcgatc agcaccccgc gccgccgccg cccggtccag
     1981 atcgccaccc ccagcgggcc gaggaactgg acggtgaccg cgctgccgag cggcaggtgg
     2041 tcgagggcga ggaagagtgc ggaggaggcg accgccgcga ccaggccgag cacgatcgcg
     2101 tcgcgccact tctcgcgcgt ccactccctc acccgaggcg actgcacggc gagcaggaag
     2161 agcgccgcga aggcgtagct gacgccgacc gcggagaccg gcgagatcgt gtcgaaggtg
     2221 gtcgtcgcca gcgccgcccc gagctggatc gaggacgcgc tggcgaggat catcgccgcc
     2281 ccgccgagct cctcccggtt cccgtgccgg cgcacgctca ggggttgaag gcgaggaaga
     2341 ggaaggcggc gaacaggatc aggtgcacgc ccccctcctg cagggtcgcg cggcccggca
     2401 gcagggtcag cgagctgacc agcgcggtca gcacgagcag cacgatctgg gttgagccga
     2461 gccccagtac cagcgggccg tcgagccaga tcgaggcgag cgcgatcacc gggatcgtca
     2521 gcccgatgct ggccatcgcc gagccgtagc cgaggttgaa gctgatctgg gtgcgcccgc
     2581 gggccgcgtt gcgcgtcgcc gccagcgtct ccggcatcag caccagcagc gcgatcacca
     2641 cgccgaccgc cgactgcggc gcgccgatcg atttcacccc gtcctcgatc gccccggagc
     2701 tgaccttggc aaggccgacc acggcgacca gcgagacagc cagcaggccg aagctgcgca
     2761 gcgctgccgc gcgggtcggc ggcgggccgt cgtgcgcggg ctcgtcctcc tcgccctccg
     2821 gcaggaagtg gctgcggtgg cggatcgtct gcacgaccag gaagaggacg tagagggcga
     2881 gcgaggcgac cgcggcgaag gcgagctgcg aggccgagaa ctccggcccg gtcttgctcg
     2941 tcgtgaaggt cggcaggacc aggctcaggg tcgccagcgt gatcacggtc gcaaacgcgg
     3001 tcgccgagcc ctcggcgttg aagcgggcga gcctttcctt catcgccgcg gtcaggatcg
     3061 ccagcccgat cacgccgttg caggtgatca tcaccgctgc gaacacggtg tcgcgtgcca
     3121 gcgtctcggt ctcgttgccg ccggcgacca tcagggtgac gatcaacccg acctcgatca
     3181 cggtcaccgc gaccgccagc accagcgagc cgtagggctc ccccacccgc tcggcgatca
     3241 cctcggcgtg gaagaccgcc gccagcaccg cgccggcgag gaagaggtcg atcacgacga
     3301 ccacggcgac cgccgggtgc gacccccagg caagggccag cgccaccagg gcgagcaacg
     3361 ggaccgcggt ccgccagctg ttcgtcaggc gctcgaagat cgagtcgagt ctattgcccg
     3421 ctccgcggcg ccccgggcgg cccgctcgcc gaccccgccg aggccgaagc ggagcgcgat
     3481 cgccgccatc aggccgcccc cgaccagcag ggtcgcgccg agcccgacgt agtcgaagag
     3541 cagccccatc gccagcgcgc cgacggtcag cccggtgatc gtcacggtta ggaagagccc
     3601 gatcatcacc cccgcgatct cttcgctgcg cagctggatg ccggtcatcc cgaccacgta
     3661 gatcaggtcc caggcgatcc cgccgaagac catcgcgacg atcgccagcg cgtaatcgta
     3721 ggtgtcgctg ccgaagatcc cgatcccggc cgccgaggtg agcccaagga tcgccagcgc
     3781 cagcgccccg gcgaagaggg cgccggagac cagcagggtg ttgcccgccc cgcgcccttc
     3841 caggtgggtg cgaatgcggt tgccgatgat gcccccgagc gccatcgcgg cgagcaggaa
     3901 gccgagcaga tgggcgcctt cgccgtgcgc ggcggcgatc gagggcgcca gctcctgaat
     3961 cggggcgacg atcagcgaga acagcgcggc gccgatcatc agatcgcgga gcaggaggtc
     4021 gcggcggacg atccgggccg cctcgcgcag gcgccggggc tgcgagcgct tgcgcggcgg
     4081 cagcgacagc ccccagacgg cgaaggcgac gaacaggtag ctgaccgcgt tcagcgcgaa
     4141 gcaccaaccg accccgagcc agacgacgag gacgccgccg agcgccggcc cgaccaggcg
     4201 cgagatgttg aaggcgaccg aggccaggcc ggtcgcccgc ttggccagct caggcgggac
     4261 cgaagcggtg accacggttt gttgcgcggc cgaggcgagc gcgccggcgg cgccgatcac
     4321 cagcacggcg aggtagatca gaagcacgct ggcgccggtc cccgcgctcc agccgaggac
     4381 cgcgagcagc gtggccgcga ccgcctcaat cgccgagagc aggatgatca gccgccgccc
     4441 gtcgtagcga tccgcgagct gcccaccgag gccgctgagc agaatccccg gtccccgcga
     4501 gacgaccgtc agcaccccca ccgcagcggc ggagttggtg atttcgtaga ccagccagcc
     4561 ggcggcgacc gtctgcatcc aggagccggc cccggagacg atgcgagcga gaaacgtgcg
     4621 gcgaaagttg gagtaggcga gcgggccctg caccccattt ttgtcggccg ccgccgggga
     4681 atcccttagc gccgaaagcg cgaacgcagt tgttcccgca tatcgggaac aactgcgttc
     4741 ggagtggcgc tacggagccg ggacgatgcg gatgtagggc ttcggcgact cccagccgtc
     4801 ggggtagcgc tctttcgcct gctcgtcgtt gaccgagccg gcgatgatca cgtcctcgcc
     4861 cggctgccac tgcgccggcg tcgccacctg gttgctggcg gtcagctgca gcgagtcgat
     4921 cacccgcagg acctcgtcga agttgcgccc ggtggtcatc gggtagacga ggatgagttt
     4981 gattttcttg tcgggcccga tcacgaagac gttgcgcagg gtctggttct gggccggcgt
     5041 gcgctcggtc gggtcgccct cgacgtcggc cggcagcatc tcgtagagct tcgagacgtt
     5101 gtggtcggtg tcggcgatca gcgggtagtt cggcgccgtc ccctgggtct cggcgatgtc
     5161 atcgctccac tgctcggtgt tctcgagcgg gtcggtggag agcccgatga ttttcacgcc
     5221 gcgcttgtcg aagtcgcctt tgatgctcgc catgtagccg agctcggtcg tgcagaccgg
     5281 ggtgaaagcg cgcgggtgcg agaacagaac cgcccagtcg tcgccgatcc actcgtggaa
     5341 gtcgatcgtc ccctccgtgg tctcggccgt gaagttcggc gcgtccgagc caatcgtcaa
     5401 tgccattgtc gagtctcctt gtctggattc cgttgcgtcc tagcttagac gccgggagac
     5461 atcgtcctca ggcccgccag ccgagccgcc gcggcctgcc gctcgtcgta ggtgacgaac
     5521 gcatcgacgg agccgagttc gaccgcggag gcgacgtgga tcgcatcgag cgtgcgcagt
     5581 gagggctcgg ccagcgcccc ggcgccgagc agaagcgcac ggtcgatcgg gtagagagcc
     5641 agctcgtcga tcagctcgcc ggcacgatcc agcgcgggtg cgagcaggaa cgtcggatcg
     5701 gccgcggcga tgcgctgcag ggcgcgcggc acctcggtca gcaccaactc gctgctggcc
     5761 agatcggttc cctcaacgtc ggcccgcaga gcgttggtct cgggctcccg gcgcaccagc
     5821 ttgaccaggg cggaggagtc cgcgtagagg agcggcactg ggtcagcgct cgtcgcgaac
     5881 ttcggcgagc gcgcgactga gggcgtacgg atcgtcgccc atctccagcg gctccggcag
     5941 gcc
//
LOCUS       DASRIG010000302         9639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3817497, whole genome shotgun sequence.
ACCESSION   DASRIG010000302 DASRIG010000000
VERSION     DASRIG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3817497"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /locus_tag="VFB52_08215"
     CDS             <1..2229
                     /locus_tag="VFB52_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZO06357.1"
                     /translation="VDRPGELELRRYLRSQRQLAARLLESETLDEVAADFLFTVANLL
                     RWDAGALWEAGEGEPILRFVSGWSVADLDAEPLWKHSRELVVESGKGLPGRAWESGEI
                     AWAPDFTRSPLYPRGATAAELGLSAALAIPIPVGRPEGVLAVAEFHARSFSPPSEELL
                     ALLGGFGDQLGTFIARRRERGHTELARNHLTQVVAGTQDAVLSKDLNGVITTWNPAAE
                     RLYGYSPEEAIGEHVSFLMPDELKHEEKEILDRVINRGESLQTYETQRVRKDGVQIDV
                     SLTVSRIEDPARGVVGASVVARDITSEIRRRRAQTFMAGASRDFDSSLDPAETARNIV
                     HAAIPDLATVCVIDFLRRDGWLGDSVVAATKPRVAAATEELRRAQPLDPDSDHPVPTA
                     LREGRPMIWRDIQKPEVLERVVQSEEQRRLIEETGYSSAATVPLVARGRTLGVLSFLH
                     AGSELRYDETDLEFLADLGDRAAMALDNARLFRERDSIARDLQRGLRPPRPAEVPGLE
                     FSVFYEAAGDGIEIGGDLYDVLPTEDGCWLLIGDVAGKGSAAAGVSVAVRHAVRGLVR
                     EVQEPEEVLNRVNEMLLEGDSLNDFATALLIRLREREDGWSAEVASAGHPPAVHVSGG
                     EAVQLGGGAVLGAWADAPPESHETTIRAGDTLVLSTDGWHEAGPVESHRDSGDLAELA
                     AELAEAELDELTAALREDALARGGGSLRDDIIVLAVRPTGEPQSSSTRSRTVVPASEE
                     RI"
     gene            complement(2172..3107)
                     /locus_tag="VFB52_08220"
     CDS             complement(2172..3107)
                     /locus_tag="VFB52_08220"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HZO06358.1"
                     /translation="MSAPTFRHEVAFYDDDDAYLATTIPFLREGLEAGEAIMVALGPE
                     KTELLQGELGAEAGEVCFEEMEEFGRNPARLIPAWREFADERRAEGAGCRGLGEPVWP
                     GRDAAEIDECLRHEALLNLAFGSDRGLALLCPYDSIALPDAVLQQALEHHPRVAGRGG
                     ACMSSPAWDDADATPDPFAGNGLVRPPGTEEFRYGVEDLHDLRAFAARAAAEAGLSDE
                     RAEDLILAASEIAANSIVHGGGRGSASAWSVPGAVLFETRDRGHIEEPLAGRVRPTPV
                     QTRGRGLWMANQLCDLVQIRSSEAGTTVRLRVELD"
     gene            complement(3204..3671)
                     /gene="soxR"
                     /locus_tag="VFB52_08225"
     CDS             complement(3204..3671)
                     /gene="soxR"
                     /locus_tag="VFB52_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HZO06359.1"
                     /translation="MISSAAMAESLTIGEVARRSGVAASALRFYEERGLIVSERAGSG
                     HRRYPRPVLRRIAFVVFAQRVGLTLEEIGEELAKLPPNRAPNRRDWSRLSAGWSDRID
                     ERIAELERLKGGLTECIGCGCLSLDRCQLANPGDRAGRAGSGPRYWVGDRPNI"
     gene            3748..4101
                     /locus_tag="VFB52_08230"
     CDS             3748..4101
                     /locus_tag="VFB52_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HZO06360.1"
                     /translation="MSTSAAPPRQRPSERITEEVTSWPGVEAGPGKRGEFAFKLNGRE
                     IGHLHGDHAAHFFFPKETWRVLHAAGRIDHHPVFPERQGPAARRIADEADVADVIALL
                     RLNYEEAQRRTRARS"
     gene            4133..5077
                     /locus_tag="VFB52_08235"
     CDS             4133..5077
                     /locus_tag="VFB52_08235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZO06361.1"
                     /translation="MKAVVVHAFGGPEVMVIDDVPTPEPIPTEVQVRVHAAGVNPVDC
                     KTRKGRGMAMVLGEPPLRIGWDVAGTVTAVGAGVTRFQVGDEVFGMPWFPRQAGAYAE
                     YVTAPSRHFSAKPAVLSIEQAAALPLAGLTAWQLVVDAIALQQGDDLLIHGAAGGVGH
                     LAVQIARARGANVFATARAEQAPMLTKLGVERTFDYRSERFEEHLADLDAVVDTTGRS
                     GTASLAALRPGGILVTVPSATEGIDELHRLAAERSQRVTGILVEPDPVGLAGLCHLVE
                     DGHLQVHVDETFDLEQVADAHRLAEGSHGGGKIVLRVP"
     gene            complement(5085..5585)
                     /locus_tag="VFB52_08240"
     CDS             complement(5085..5585)
                     /locus_tag="VFB52_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06362.1"
                     /translation="MASALVASACAAAQAQAIHYEATNPPGTLSGSANAGEELITEAG
                     SVYCEGPSLSEACKNGPFHSLSLHPPFTHCDIECDSRLESIKVTASTCSMEIRDAAAN
                     SSFTQATILHGGESLLFSPKSPTSPPTTASSARSAAKAKTRAANTNRAERLRSSPRPA
                     RTTSSR"
     gene            complement(5621..6277)
                     /locus_tag="VFB52_08245"
     CDS             complement(5621..6277)
                     /locus_tag="VFB52_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06363.1"
                     /translation="MNIRRLGRAALPVSAALLVLLGTLAPASQAAPLFEATESSAVIA
                     TPGGDTEIALEGETISIVCVSDVQTGSYSSTSFPTLMLHPSLGTCELVGFSAATVNTE
                     SCDFAYSPTKRIGADLYNASFGIVCPSGDAIRITAATCALSINGGGSNEGLTNVQLAN
                     SGPGVEITPLVGGISYKVTNDGFFCPFDGKGDRTDGSYTAVSGLGAIPMSGGLGVAGT
                     "
     gene            complement(6274..6924)
                     /locus_tag="VFB52_08250"
     CDS             complement(6274..6924)
                     /locus_tag="VFB52_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06364.1"
                     /translation="MISKRLKTAVLTLLVTCAVGALAPTAQADPVFEASNSSGTVSGS
                     GNGFEQFVTEAGTITCSVGNYTGSYSSKTFSTQRLHPSYSSCSAFGFLSATVNTEDCD
                     FILDPYEKVVTWGYHLRMAIDCPAGDSIKVTASTCSMRIEDTAANQNLTKIVSLDASG
                     GIILEPEVIWIQYNVTNDGFLCPFFGTGIKNDGRFDPTAVIPITAAGGATITVVGE"
     gene            complement(7126..>9639)
                     /locus_tag="VFB52_08255"
     CDS             complement(7126..>9639)
                     /locus_tag="VFB52_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZO06365.1"
                     /translation="ADHELAGLCRQRFGDRYDGNRARAEAEARLDTELGTIRHLGLSG
                     FFLLHYELLELAREVAVEVRGPDSARSLLPPGRGRGSSVSSIVCYLTGLSHIDPVENK
                     LFAGRFLNDEAASLPDIDLDFPRDIRAVLIPRIHEVYGADRSALVAAFPTYRPRGVVR
                     DLGKALGLPPAEIEKVAGVVGFHDSAGELARDVVAAIGTERAAAPRWRALLWLSEDAL
                     GLPRHASQHSGGMVISTEPLVDVCPVVPAAMTGRQIVQWDKDSCADAGFLKIDLLGLG
                     MLSAVERCVEELGRTRGERLDLSRIDYDDAGTFDSIRKAETTGVFQIESRAQMQMLPR
                     TRPRDLDDLTVQVALIRPGPIQGGAVHPYIERRKLARENPGYEVPYEHESLRPVLAET
                     LGTIIYQEQVLEVAIVFAGFTSGEAESLRRAMSRKRSLQAIERHHRRFIEGAVALHGV
                     EPQLAERIWDKIQGFSGFGFPKAHSAAFGLLAYQSAWLRFHRGPEFLCALLNEQPMGF
                     YPPDSLAHEAQRRGLRVAPPDVNRSRVLCHVESPRENPGLRGGMVVRIGLGYVKGVRE
                     EEMEALVAARESGSGGYYGGVADLASRSGAGLASLERLAWAGALDGIPVGAEGERREA
                     LWRVGVVANSGGRDGAQLGLPIEPPAPPPLEPLGEWAQLIADYRSTGMVLGKHPMELL
                     RPGLTPAIRRSVELAKVEDGAEVEVAGMVVARQRPETAKGIVFMLFEDESGVVNLVVP
                     SKVYERFRALVRGAPLLAARGCLERREGVVNVVVAELWELEGSAEATEPPPTSARPER
                     REPPPGSGRDPVPRRQRQHAVAELRAVAPAGHAFGRRGR"
BASE COUNT         1441 a         3283 c         3473 g         1442 t
ORIGIN      
        1 gtggatcgcc ccggggaact cgagctgcgg cgctacctgc gcagtcagcg gcagctggcg
       61 gcccgcctgc tcgagagcga gaccctcgac gaagtcgccg ccgacttcct cttcaccgtc
      121 gccaacctgc tgcgctggga cgcgggcgcg ctttgggaag cgggcgaggg cgagccgatt
      181 ctgcgcttcg tctccggctg gagcgtcgcc gacctcgacg ccgagccgct ctggaagcac
      241 agccgcgagc tggtcgtgga gagcggcaag ggcctgccgg gacgcgcctg ggagtccggc
      301 gagatcgcct gggcgcccga cttcacccgc agcccgctct acccgcgggg ggcgacggcg
      361 gccgagctcg gcctcagtgc cgcgctcgcg atcccgatcc cggtcggtcg cccggagggg
      421 gtgctggcgg tggccgagtt ccacgcgcgc tcgttcagcc ctccctccga ggaactgctg
      481 gcgctgctcg gcggcttcgg cgaccagctc ggcaccttca tcgcccggcg ccgcgagcgg
      541 ggccacaccg agctcgcccg caaccacctc acgcaggtgg ttgccgggac ccaggacgcg
      601 gtcctcagca aggacctcaa cggcgtgatc acgacctgga acccggccgc cgaacgcctc
      661 tacggctaca gccccgagga ggcgatcggc gaacacgtct ccttcctgat gcccgacgag
      721 ctcaagcacg aggagaagga gatcctcgac cgggtgatca accgaggcga atctctgcag
      781 acctatgaga cgcagcgggt gcgcaaggac ggggtgcaga tcgacgtctc gctgacggtc
      841 tcgcggatcg aagacccggc ccgcggcgtg gtcggcgcct cggtcgtcgc ccgcgacatc
      901 accagcgaga tccggcggcg gcgcgcgcag acgttcatgg ccggcgccag ccgcgatttc
      961 gactcctcgc tcgacccggc ggaaacggcg cgcaacatcg tccacgcggc gatccccgac
     1021 ctcgccacgg tctgcgtgat cgactttctg cggcgcgacg gctggctcgg cgacagcgtt
     1081 gtcgccgcga ccaagccgcg ggtggcggcg gcgaccgagg aactgcgccg cgcccagccc
     1141 ctggatcccg acagcgacca cccggtgccg accgcgctgc gcgaggggcg gccgatgatc
     1201 tggcgcgaca tccagaagcc ggaggtgctg gagcgggtgg tgcagagtga ggagcagcgc
     1261 cggctgatcg aggaaaccgg ctacagctcc gccgctacgg tgccgctggt cgcgcgcggc
     1321 cgcacgcttg gggtgctctc attcctgcac gcggggagcg agctgcgcta cgacgagacc
     1381 gacctcgagt tcctcgccga cctcggcgac cgggcggcga tggcgctcga caacgcgcgc
     1441 ctgttccgcg agcgcgactc gatcgcccgt gacctgcagc gcgggctgcg cccgccgcgg
     1501 ccggccgagg tgccggggtt ggagttctcg gtcttctacg aggcggccgg agacgggatc
     1561 gagatcggcg gcgacctcta cgacgtgctg ccgaccgagg acggctgctg gctgctgatc
     1621 ggcgacgtcg ccggcaaggg cagcgccgcg gccggcgtct cggtcgccgt tcgccacgcg
     1681 gtgcgcggcc tggtccgcga ggtgcaggag cccgaggagg tgctgaaccg ggtcaacgag
     1741 atgctgctcg agggggacag cctcaacgac ttcgcgaccg cgctgctgat ccggctgcgc
     1801 gagcgcgagg atggctggag cgcggaggtc gccagcgccg gccacccgcc ggccgtgcac
     1861 gtgagcggcg gcgaggcggt ccagcttggt ggcggcgcgg tcctgggcgc ctgggcggat
     1921 gcgccgccgg aaagccacga aacgacgatc agggcgggcg acacgctggt gctcagcacc
     1981 gacggctggc acgaggccgg gccggtggag agccaccgcg attcgggcga cctggccgag
     2041 ctggccgccg agctggccga ggccgagctg gacgagctga ccgcggcgct gcgcgaggat
     2101 gcgctggcgc gcggtggcgg ttcgctgcgc gacgacatca tcgtgctcgc ggtgcgcccg
     2161 accggcgagc ctcagtcgag ctcgacccgc agccgcacgg tcgtgcccgc ctccgaagag
     2221 cggatctgaa ccaggtcaca gagctggttg gccatccaga ggccgcgccc tcttgtctgg
     2281 accggcgtcg gccgcacccg cccggccagc ggctcttcga tgtggccgcg gtcgcgcgtt
     2341 tcgaacagca ccgcgccggg caccgaccag gcgctggcac tgccccggcc accgccgtgg
     2401 acgatgctgt tggcggcgat ctcgctggcg gcgaggatca ggtcttcggc gcgctcgtcg
     2461 gagagccccg cctccgcggc tgcgcgggcg gcgaaggcgc gcaggtcgtg caggtcctcg
     2521 acgccgtagc ggaactcttc ggtgccgggg ggacgaacga gcccgttgcc ggcaaacggg
     2581 tcaggggtgg cgtcggcgtc atcccaggcg gggctgctca tgcaggcgcc gccgcgtccg
     2641 gcgacccgcg ggtggtgctc cagggcttgc tgcagaaccg cgtccgggag cgcgatgctg
     2701 tcgtagggac atagcagcgc gaggccgcgg tcggagccga aggcgaggtt gagcagcgcc
     2761 tcgtggcgca ggcactcgtc aatctcggcg gcgtcgcggc cgggccacac cggttcgccg
     2821 agcccgcggc agccggcgcc ctccgcccgg cgttcgtcgg cgaactcgcg ccaggccgga
     2881 atcagccgcg ccgggttgcg gccgaactcc tccatctcct cgaagcagac ctccccggct
     2941 tcggcgccga gctcgccctg cagcagctcc gtcttctccg gtccgagcgc gaccatgatc
     3001 gcctcgccgg cctcgagccc ctcgcgcagg aacgggatcg tcgtcgccag gtatgcgtcg
     3061 tcgtcgtcgt agaaggcgac ttcgtgacgg aaagtgggcg cagacacggc caaggcatac
     3121 ccattgaagc gagaacttga accaatccca acccagactt ttggccattg ggagcggaat
     3181 ttcagccgaa tactgaacag tgatcagata ttcggacgat cgcctaccca gtatcgagga
     3241 ccggatccgg cacggccggc acggtcgccc ggattagcca gctggcagcg atcgagtgag
     3301 aggcatccgc acccgatgca ctcggtgagc ccgcccttga gccgttcgag ctcggcgatc
     3361 cgctcgtcga tgcggtcgct ccacccggcc gagagccgcg accagtcgcg gcggttgggc
     3421 gcccggttcg ggggcagctt ggcgagctct tcgccgatct cctccagggt cagcccgacc
     3481 cgctgggcga agacgacgaa ggcgatccgc cgcagcaccg ggcgcgggta gcgccggtgg
     3541 ccggagccgg cgcgctcgga gacgatcaag cctcgctcct cgtagaagcg cagcgccgag
     3601 gcggcgacgc cactgcgccg cgccacctcg ccgatcgtta gcgattcggc catcgccgct
     3661 gaggaaatca cgcttcgccc ttgacttcaa gttaacttga acttgtagcg tcggtgccga
     3721 tcgccgaacc gagacgagga ggaaccgatg agcaccagcg cagcaccgcc acgccagcgg
     3781 ccgagcgagc ggatcaccga ggaggtgacc tcctggcccg gggtcgaggc cggcccgggc
     3841 aagcgcggcg agttcgcctt caagctgaac gggcgcgaga tcggccacct gcacggcgac
     3901 cacgcggcgc acttcttctt cccgaaggag acctggcgcg tgctgcacgc ggccgggcgg
     3961 atcgaccacc acccggtctt ccccgagcgc cagggaccgg ccgcgcggcg gatcgccgac
     4021 gaggccgacg tcgccgacgt gatcgcgctg ctgcggctga actatgagga ggcacaacgg
     4081 cgcacccgcg cgcgctccta gggccgcgcg aaaccctcta ggttgggggt ggatgaaggc
     4141 agtggtcgtg cacgcgttcg gcggtcccga ggtgatggtg atcgacgacg tgccgacgcc
     4201 cgagccgatc ccgaccgagg tccaggtccg cgtccacgcc gccggcgtca acccggtcga
     4261 ctgcaagacc cgcaagggcc gcgggatggc gatggtgctc ggcgagccgc cgctgcggat
     4321 cggctgggac gtcgccggga cggtcaccgc ggtcggcgcc ggggtgaccc gcttccaggt
     4381 cggcgacgag gtctttggga tgccctggtt cccgcgccag gccggcgcct acgccgagta
     4441 cgtgaccgcg ccctcgcgcc acttctcggc caagccggcg gtgctctcga tcgagcaggc
     4501 ggcggcgctg ccgctggccg gactgaccgc ctggcagctc gtcgtcgacg cgatcgccct
     4561 gcagcagggg gacgacctgt tgattcacgg tgccgccggc ggtgtcggcc acctcgccgt
     4621 gcagatcgcc cgggcgcgcg gcgccaacgt cttcgccacc gcccgcgccg agcaggcgcc
     4681 gatgctgacc aagctcggcg tcgagcgcac cttcgactac cgcagcgagc gcttcgagga
     4741 gcacctcgcc gacctcgacg cggtggtcga caccaccggc cgcagtggca ccgcctcgct
     4801 cgccgcgctg cgccccggcg ggatcctcgt caccgtgccc agcgccaccg agggcatcga
     4861 cgagctgcac cggctcgccg ccgagcgcag ccagcgcgtc accggcatcc tggtcgagcc
     4921 cgacccggtc ggcctcgccg gcctctgcca cctggtcgag gacggccacc tgcaggtcca
     4981 cgtcgacgag accttcgatc tcgagcaagt tgccgacgcc caccggctcg ccgaaggcag
     5041 ccacggtggc ggcaagatcg tgctgcgcgt gccatagccg gaggtcatcg cgacgatgtc
     5101 gttcgcgccg ggcgaggtga agacctcaac cgttctgctc gatttgtgtt tgccgcccgt
     5161 gtttttgcct ttgccgctga acgggcagag gaagccgtcg ttggtggcga cgtaggcgat
     5221 ttcgggctga acaggagcga ttcgccaccg tgcaggatcg tcgcttgcgt gaagctggag
     5281 ttcgccgccg catccctgat ttccatcgag caggtcgagg ccgtgacctt gatcgattcg
     5341 agtctcgagt cgcactcgat gtcgcagtgt gtgaagggcg gatgcaggct cagagaatgg
     5401 aatggcccgt ttttacaggc ctcgctgagc gagggtcctt cgcagtagac cgaccccgct
     5461 tcggtgatga gctcctcgcc ggcgttcgcg gacccggaaa gggtgccggg cgggttggtg
     5521 gcttcgtagt gaatcgcctg ggcctgcgcg gcggcgcatg ccgaggccac cagcgccgat
     5581 gccaacgtga acgcccgtca atgggcaggg catcccagtt tcatgtgccc gccactccta
     5641 gtccgccaga catagggatt gcgcccaggc cagataccgc tgtgtagctg ccgtcggttc
     5701 tgtctccttt tccgtcgaac gggcagaaga acccatcatt agtcactttg tatgagatcc
     5761 cgccgaccaa gggcgtgatc tcgaccccag gtccactgtt cgccaactgt acgttggtca
     5821 gcccctcatt cgatcctccg ccgttgatgc tcagagcgca ggtagcggcg gtgatcctga
     5881 tggcgtcgcc agatgggcag acgatgccga agctcgcgtt gtagaggtcg gcgccaattc
     5941 gtttggtagg cgagtacgcg aagtcgcagg attccgtatt gaccgtggca gcactgaatc
     6001 cgaccagttc acaggtccca agacttgggt gcagcatcag agtcgggaac gaggtggatg
     6061 aatagcttcc ggtttgaaca tcggatacac acacgatgct gatcgtttcg ccttcgaggg
     6121 ctatttccgt atccccaccg ggagttgcta tcacggcact cgattcagtc gcttcaaaga
     6181 gaggtgctgc ttggcttgcc ggcgcaagcg tgccaagcag cactagaagt gccgcgctga
     6241 ccgggagggc tgcacgccca agtcgacgaa tgttcattcg ccgaccactg tgatcgtcgc
     6301 accgcctgcg gctgtgatcg ggatcacggc ggtggggtcg aatctcccat cgttcttgat
     6361 ccccgtgccg aagaaggggc agaggaagcc gtcgttggtg acgttgtact ggatccagat
     6421 gacttcaggt tcgagaatga tgcccccgct tgcgtccagg ctgacgattt tcgtgaggtt
     6481 ttggtttgcc gccgtgtctt cgattcgcat cgagcaggtg cttgcggtga ctttgatcga
     6541 atctccagcg gggcagtcga tcgccatgcg caggtggtag ccccaggtga cgactttttc
     6601 gtatgggtcc aggatgaagt cgcagtcttc ggtgttgacg gttgcgctca ggaatccaaa
     6661 cgcgctgcat gacgagtagg aggggtggag ccgctgcgtt gagaaggttt tggagctgta
     6721 gctgccggtg tagttgccaa ccgagcaggt gatcgtgccg gcttcggtga cgaactgttc
     6781 aaaaccattg cccgagccgg agacggttcc cgaggagttc gaggcttcga aaacggggtc
     6841 ggcttgggcg gtaggcgcca gagcccctac ggcacaggtc acgagcaagg tcagaactgc
     6901 ggttttcagc ctcttcgaga tcatcggttc ctctcctttg ggtacgcctt gatctataga
     6961 gcggccgccg gggctcgggc aaggaggatg gccccgcgaa ggccaaggct gggatcgaac
     7021 ggtgtgatcc gcctgcaaat ccgctgcggc gtattcccgc gtgcgccgat ccgtgcagcg
     7081 cagcatgtcg cgcttcgcac ggaatagaca ggactcccga tccggtcagc ggccccgcct
     7141 gccgaaggcg tggccggcgg gagcgactgc gcggagctcg gcgacggcat gttgccgctg
     7201 gcggcggggg acggggtcgc ggccgctgcc ggggggcggt tcgcggcgct cggggcgggc
     7261 cgaggtcggc ggcggttcgg tcgcctcggc gctgccctcc agctcccaga gctcggcgac
     7321 gacgacgttg acgacccctt cgcggcgctc gaggcagccg cgggcagcga gcaggggggc
     7381 gccgcggacg agggcgcgga agcgctcgta gaccttggac ggcacgacca ggttgacgac
     7441 tccggactcg tcctcgaaga gcatgaagac gatccccttt gcggtctcgg ggcgctggcg
     7501 ggcgacgacc atgccggcga cctcgacctc ggcgccgtcc tcgaccttgg ccagctcgac
     7561 gctgcggcga atcgctgggg tgagtcccgg gcgcagtagc tccatggggt gcttgccgag
     7621 caccatcccg gtcgagcggt agtcggcgat cagctgcgcc cactcgccga gcggctccag
     7681 cggcggcggc gcgggtggct cgatcggcag gccgagctgg gcgccgtcgc ggccgccgct
     7741 gttggcgacc acgccgaccc gccagagcgc ctcgcggcgc tcgccctcgg cgccgaccgg
     7801 gatcccgtcg agcgcccccg cccaggccag ccgttcgagg ctcgccagcc ccgcccctga
     7861 gcgggaggcg aggtcggcga cgcccccgta gtaaccgccg ctgccgctct cgcgcgccgc
     7921 gaccagcgcc tccatctcct cctcgcgcac ccccttcacg tagccgagcc cgatccgcac
     7981 caccatgccg ccgcgcaggc ccgggttctc gcgcggcgat tcgacgtggc agaggacgcg
     8041 gctgcggttg acgtcgggtg gggcgacgcg gaggccacgc cgctgcgcct cgtgggccag
     8101 cgagtcgggc gggtagaagc ccatcggctg ctcgttcagc agcgcgcaga ggaactcggg
     8161 cccgcggtgg aagcgcagcc aggccgactg gtaggcgagc aggccgaagg cggccgagtg
     8221 cgccttcggg aagccgaagc cggagaagcc ctggatcttg tcccagatcc gctcggccaa
     8281 ctgcggctcg accccgtgca gcgcgaccgc cccctcgatg aagcggcggt ggtggcgctc
     8341 gatcgcctgc agcgagcgct tgcggctcat cgcccggcgc aggctttcgg cctcgccgga
     8401 ggtgaagccg gcgaagacga tcgccacctc cagcacctgc tcctggtaga tgatcgtgcc
     8461 gagcgtctcc gccagcaccg ggcgcagcga ctcgtgctcg tagggaacct cgtagccggg
     8521 gttctcgcgg gccagcttgc gcctctctat atatgggtgg accgcgccgc cctggatcgg
     8581 gccgggccgg atcagcgcca cctgcacggt caggtcgtcg aggtcgcgcg ggcgcgtgcg
     8641 cggcagcatc tgcatctgcg cgcggctctc gatctggaag acgccggtgg tctcggcctt
     8701 gcggatcgag tcgaaggtgc ccgcgtcgtc gtagtcgatc cgcgagaggt cgagccgctc
     8761 gccgcgggtg cgcccgagtt cctcgacgca gcgctcgacc gcggagagca tgccgagccc
     8821 gagcaggtcg atcttgagga agccggcgtc ggcgcaggag tccttgtccc actggacgat
     8881 ctggcgtccc gtcatcgccg ccggcacgac cgggcagacg tcgaccaggg gctcggtcga
     8941 gatcaccatc ccgccggagt gctgggaagc gtggcggggc agcccgagcg cgtcctcgct
     9001 gagccagagc agcgcccgcc agcgcggcgc ggcggcacgc tcggtgccga tcgcggcgac
     9061 cacgtcgcgg gccagctcgc cggccgagtc gtggaagccg acgaccccgg cgaccttctc
     9121 gatctccgcg ggcggcagcc ccagcgcctt gccgaggtcg cggacgacgc cgcgcgggcg
     9181 gtaggtcggg aaggcggcga ccagggccga gcggtcggcc ccgtagacct cgtggatgcg
     9241 cgggatcagg accgcgcgga tgtcgcgcgg gaagtcgagg tcgatgtccg gcagcgaggc
     9301 cgcttcgtcg ttgaggaagc gaccggcgaa gagcttgttc tcgaccgggt cgatgtggga
     9361 gaggccggtc aggtagcaga cgatcgagct gacgctggag ccgcgcccgc gcccgggcgg
     9421 caacagcgag cgcgctgagt cggggccgcg cacctcgacc gcgacctcgc gggcgagctc
     9481 gagcagctcg tagtggagga ggaagaagcc ggagaggccg aggtggcgga tcgtccccag
     9541 ctcggtgtcg agccgggcct cggcctcggc ccgcgcgcgg ttgccgtcgt agcggtcgcc
     9601 gaagcgctgg cggcagagcc cggcgagctc gtggtcggc
//
LOCUS       DASRIG010000303         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_680682, whole genome shotgun sequence.
ACCESSION   DASRIG010000303 DASRIG010000000
VERSION     DASRIG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680682"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFB52_08260"
     CDS             complement(<1..212)
                     /locus_tag="VFB52_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06366.1"
                     /translation="MALDRGGALSGIPAKFRSLGNKINGGEMISSSPPKRSWLRLLAV
                     GLGVVLIAALLAACGGSDSDSTSSGS"
     gene            322..1620
                     /locus_tag="VFB52_08265"
     CDS             322..1620
                     /locus_tag="VFB52_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZO06367.1"
                     /translation="MNDHQLTPTGKNLDLSFLQKPHEHDPRRIYSTTGTDWQSRVDFE
                     RMRKDRLARARLKMEENDLGAVVCFVGENVRYITGVFQGNWKNNIFIRYCVLPRGGEP
                     VLFETAGSDLECAKIDAPWLKGNIRPAITWKWAETAEQMMADKMVQSIKDVLSENGVA
                     EEKIGIDIYDPSAAAAFDRAGIQTTSAWPVMSKARVVKTPDELECLKISSAIGDTSFW
                     KIENEWLKPGVKESFITAKVNEYLYEHGFDFVYDIIVASGGNTSPYRRWHTDKVIREG
                     DLVIVDQNAVGPGGYFVDFVRTFKCGGGKWTEKEKDLYKECYETMYAAIEEMKAGNTT
                     ADVAAKLPTYADDEFGTVTLQQFAHSIGLSLYEGMWISRAYSLDYPVELEENMYFAAE
                     TFAGHPGLEQTVRLEENLLVTKDGPVLFTRHPFYDDILAS"
     gene            1625..2581
                     /locus_tag="VFB52_08270"
     CDS             1625..2581
                     /locus_tag="VFB52_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HZO06368.1"
                     /translation="MAKRVVITDHVFGGLDIERETLEPLGAEVVLAASSDEDTLVDLA
                     STADAMLVCFAAVGAPVVRAAGENGCAVIARYGIGVDNIDIPTATELGIRVTNVPDYC
                     LDEVADHTLALLLCAARQLVPANLGVREGDWTIPHGNVHRLRGRRLALLGVGRIGSRV
                     VERALPLGLEVVGYDPFLTEPIPGLIQVGSAAEAVREADFVSLHAPLTDETHHLIGPE
                     LIAEMERAPVVINTSRGPLVDPDAVVAALAAGTLSGVALDVTEVEPLPDEHPLRTHPQ
                     AVVTPHMAFYSVEAERELQERAADEVARALRGEPARSPVNDV"
     gene            2578..>2764
                     /locus_tag="VFB52_08275"
     CDS             2578..>2764
                     /locus_tag="VFB52_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06369.1"
                     /translation="MSAPAAQFPDLAGKVAVVTGGSAGIGAATCELLAANGAKVAVVG
                     RRREPIDALLERLGGEAI"
BASE COUNT          503 a          933 c          889 g          439 t
ORIGIN      
        1 tcggaaccgc ttgaggtgct gtccgaatcg gatccgccgc aggcggccag cagggccgcg
       61 atcagaacga ctccgaggcc gactgcaagc aggcgcagcc acgacctttt aggtggacta
      121 ctgctgatca tctctcctcc atttattttg ttacccaggc ttctgaattt ggccggaatg
      181 cccgaaaggg cccctcctct gtccagtgcc attactacgg gtatcagata tgaagcaaga
      241 ctgcaagatt tgcaatctga aaacaggtgt gcttacactc tgacttcaaa ttgctgtcga
      301 cttagggacc ggagagaact agtgaacgac caccagctga cgcccactgg aaagaacctg
      361 gacctttctt tcctgcaaaa gccgcacgag cacgatccgc ggcggatcta cagcacgact
      421 gggaccgact ggcagtcgcg cgtcgatttc gaacggatgc gcaaggaccg gctggcccgc
      481 gcccgcctga agatggagga gaacgacctc ggcgccgtgg tctgcttcgt cggcgagaac
      541 gtccgctaca tcaccggcgt cttccagggc aactggaaga acaacatctt catccgctac
      601 tgcgtgctgc cccgcggcgg cgagccggtg ctgttcgaga ccgccggctc cgacctcgag
      661 tgcgcgaaga tcgacgcccc ctggctgaag ggcaacatcc gcccggcgat cacctggaag
      721 tgggccgaga ccgccgaaca gatgatggcc gacaagatgg tccagtcgat caaggacgtg
      781 ctgagcgaga acggcgtcgc cgaggagaaa atcggcatcg acatctacga cccgagcgcc
      841 gcggccgcct tcgatcgagc cgggatccag accacgagcg cctggccggt gatgtcgaaa
      901 gcccgcgtcg tcaagacccc ggacgagctc gagtgcctga agatctcctc cgcgatcggc
      961 gatacctcgt tctggaaaat cgaaaacgaa tggctgaaac cgggggtcaa ggagtccttc
     1021 atcaccgcga aagtcaacga gtacctctac gagcacggct tcgacttcgt ctacgacatc
     1081 atcgtcgcct cgggcggcaa caccagcccc tatcgccgct ggcacaccga caaggtgatc
     1141 cgcgaaggcg acctcgtgat cgtcgaccag aacgcggtcg gcccgggcgg ctacttcgtc
     1201 gacttcgtgc gcaccttcaa gtgcggtggc ggcaagtgga cggagaaaga aaaggacctc
     1261 tacaaggagt gctacgagac gatgtacgcg gcgatcgaag aaatgaaggc gggcaacacg
     1321 accgccgacg tcgccgcgaa gctgccgacc tacgccgacg acgagttcgg cacggtcacc
     1381 ctgcagcagt tcgcgcactc gatcggcctc tcgctctacg agggcatgtg gatctcgcgc
     1441 gcctactcac tggactatcc ggtcgagctg gaggagaaca tgtacttcgc cgccgagacg
     1501 ttcgccggcc accccggcct cgagcagacc gtgcggctgg aggagaacct gctggtgacg
     1561 aaagacgggc cggtgctgtt cacccgccac ccgttctacg acgacatcct cgcctcctag
     1621 ccagatggcg aagcgagtcg taatcaccga tcacgtcttc ggcggcctcg atatcgagcg
     1681 cgagacgctc gagcccctgg gcgccgaagt ggtcctcgct gcctcctctg acgaggacac
     1741 cctggtcgat ctggcgtcca ccgccgacgc gatgctcgtc tgcttcgctg cggtgggggc
     1801 gccggtcgtc agggccgcgg gcgagaacgg gtgcgcggtg atcgcccgtt acggcatcgg
     1861 cgtcgacaac atcgacatcc cgaccgcgac cgagctcggg atccgggtca ccaacgtgcc
     1921 cgactactgc ctcgacgagg tcgccgacca caccctggcg ttgctgctct gcgcggcgcg
     1981 ccagctggtg ccggccaacc tcggcgtgcg cgagggcgac tggacgattc cgcacggcaa
     2041 cgtgcaccgg ctgcgtggcc gccggctcgc cctgctcggg gtcggccgga tcggcagccg
     2101 ggtggtcgag cgggcgctgc cgctcgggct cgaggtggtc ggatacgatc ccttcctgac
     2161 cgagccgatc cccggcctga tccaggtcgg gtcggcggct gaggcggtca gggaggcgga
     2221 ttttgtgtcc ttgcatgcgc ccctcaccga cgagacccac cacctgatcg gacccgagtt
     2281 gatcgccgag atggagcggg caccggttgt gatcaacacc tcgcgggggc ccctggtcga
     2341 ccccgacgcc gtagtcgccg cgctggccgc cggcacgctc tccggggttg ccctcgacgt
     2401 caccgaggtt gagcccctgc ccgacgagca cccgttgcgc acccatccgc aggcggtcgt
     2461 cactccgcac atggccttct actcggtcga ggccgagcgg gagctgcagg aacgcgccgc
     2521 cgacgaggtg gcgcgagcgc tgcgcggcga accggctcgc tctcccgtca acgacgtatg
     2581 agcgcgccgg ccgcacagtt ccccgacctc gccggcaagg tcgccgttgt caccggcggc
     2641 tcggccggga tcggtgcggc cacctgcgag ctgctcgccg cgaacggcgc caaggtcgcg
     2701 gtcgtcggcc gccgccgcga gccgatcgac gccctgctcg agcggctcgg cggcgaggcg
     2761 atcg
//
LOCUS       DASRIG010000304         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_680947, whole genome shotgun sequence.
ACCESSION   DASRIG010000304 DASRIG010000000
VERSION     DASRIG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680947"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFB52_08280"
     CDS             <1..697
                     /locus_tag="VFB52_08280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06370.1"
                     /translation="GGIGLAIARVLGEEGYGLTLSGRRAEKLEQEVQGLRDDGFEVLT
                     VPANMADEDDLLSLFAAHKERYGRLDALVNNAGVGIGAPMAEIQTKFLDMQLGVNLRA
                     LIIGTREGLPMLREAGAEHGKALIINTASLAGKAGPAWLSIYGATKAAVISFSESTQK
                     EVARDGIQCTALAPGFVDTAMAEFIKDQIGEELIQPSDIGEAVRFLLRTSRSCHVPEI
                     VFVRPGESIVNPA"
     gene            804..1910
                     /locus_tag="VFB52_08285"
     CDS             804..1910
                     /locus_tag="VFB52_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZO06371.1"
                     /translation="MVETWEERRAAVEGAGLGFAAAEEYRMFPPPEPDSADGQHAAEA
                     ARALLPLLEEMRPHVAVSDILTLAPALAAEAAGVPLATLIPHIYPVVEPGLPFFAIGL
                     RKPRTPLGRAIWRTGQRALDVGLEHGRRDLNSQRERLGLPPTERFHGGISPDLALVAT
                     YPQLEYPRRWPAGVRVTGPMIFEQPFAEIELPPGGDPLVLVAPSTAHDSDNQLVRTAL
                     AALAEEPVRVVATTNRVKPQQPIEVPANAKLVDWLSYSQLMPAAALVISHGGHGTVAR
                     ALGAGTPVLISPITGDMSETAMRVDWAGAGLSLPWRLCRPTPLRWAALRLLHEPGFTA
                     RAEAIAAWGRENDGAVRGAELVEQLAAAKASALT"
     gene            complement(1929..2001)
                     /locus_tag="VFB52_08290"
     tRNA            complement(1929..2001)
                     /locus_tag="VFB52_08290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1966..1968),aa:Asn,seq:gtt)
     gene            complement(2056..3855)
                     /gene="dnaG"
                     /locus_tag="VFB52_08295"
     CDS             complement(2056..3855)
                     /gene="dnaG"
                     /locus_tag="VFB52_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZO06372.1"
                     /translation="MALISPESLDRLKQTVDIVEVISAHTDLRRQGTRMVGLCPFHEE
                     RTPSFSVDPQEKLYHCFGCGVGGDAIKFVEEKEGLGFAEAVELLADRYGVELERERED
                     PRAEARRQQRRRLEALLDRTASYYANYLWDSKEAGKAREYLAERGLGEQVLRDFGVGY
                     APSAWDKILVAGQRAGFSVEELRGVGLAQRGRQGGEYDRFRSRIMFPIRDRRGRTLGF
                     GGRAMRSDQGAKYVNTAETDFFHKSQMLYGVDRAKAAIAKAGRAVVVEGYTDVLALHQ
                     AGVEEAVGVMGTAITDDQVKELSGMVDEVVLALDADSAGQEAMLRAQRVAAGRRMRLR
                     VAAMPDGIDPAEMMAEPDGPERFRALADGAEELTSFQVALVLARTDVSSPVERDRALE
                     EVAPVLAGMQEGAMRDDLIRRVAERLDFEPATVMGRVVAAEPASGGEQAAGQAYESFS
                     AEAQSQPARQPRRAAELTSRERRERALLAMCIALPDDGREFLARLTDAHLSASGIRAA
                     AWLRENPGDPASNLPQDDEMASLISELVMLSHDQPASRESMELNFLLLEQRRLEDEIA
                     AAGERGDYEHRAALSRERAALVEKIAHTQRVES"
     gene            complement(3855..>4427)
                     /locus_tag="VFB52_08300"
     CDS             complement(3855..>4427)
                     /locus_tag="VFB52_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZO06373.1"
                     /translation="VDVLERDGEGLNLTEQVRDGILNHTGPNKPATLEGRIVKLVDRV
                     AYINHDIDDALRAGILRPEDLPAAEIELLGPTGSKRIDTLVIDIVENSREAGDIVQSE
                     EIGMAMLRLRKFMFERVYLGPDARREHDRVQRAMRGLFEHYLEHPEELPEWDAGASPC
                     QRVADYIAGMTDRYCIAKFTELTVPEESRF"
BASE COUNT          595 a         1629 c         1565 g          638 t
ORIGIN      
        1 cggcggcatc gggctggcga tcgcgcgggt gcttggcgag gagggctacg gcctcaccct
       61 ttccgggcgc cgcgcggaga aactcgagca ggaggtccag gggctgcgcg acgacggctt
      121 cgaggtcctc accgtccccg ccaacatggc cgacgaggac gacctgctgt cgctgttcgc
      181 cgcccacaag gagcgctacg gccgcctcga cgcgctggtc aacaacgccg gggtcgggat
      241 cggcgcgccg atggccgaga tccagaccaa attcctcgac atgcagctcg gggtcaacct
      301 gcgggcgctg atcatcggca cccgcgaggg cctgccgatg ctgcgcgagg ccggcgccga
      361 gcacggcaag gcgctgatca tcaacaccgc ttcgctggcc ggcaaagccg gcccggcctg
      421 gctctcgatc tacggcgcga ccaaggcggc ggtgatcagc ttctccgaat cgacccagaa
      481 agaggttgcc cgcgacggca tccagtgcac ggcgctcgcc cccgggttcg tcgacacggc
      541 gatggccgag ttcatcaaag accagatcgg cgaggagctg atccagccct ccgacatcgg
      601 cgaggcggtc cgcttcctgc tgcgcacctc gcgcagctgc cacgtccccg agatcgtctt
      661 cgtccgcccc ggcgagagca tcgtcaaccc tgcctgaacc gggcgcaccg ctgcgggtcc
      721 tggtcgccgc cttcggcgac gccggtcacg ccttcccggc gatcgccctg ggacgggcgc
      781 tggcggcgcg cgggcacgag gtcgtggtcg agacctggga ggagcgccgc gccgccgtcg
      841 agggggccgg gctcggcttc gccgcggccg aggaataccg gatgttcccg ccgcccgagc
      901 ccgactcggc cgacggccag cacgccgccg aggcggcgcg ggcgctgctg ccgctactgg
      961 aggagatgcg cccgcacgtc gccgtcagcg acatcctcac gctcgcgccg gcgctggcgg
     1021 cggaggccgc cggggtgccg ctggcgaccc tgatcccgca catctacccg gtcgtcgagc
     1081 cgggcctgcc gttcttcgcg atcgggctac gcaagccgcg cacgccgctc gggcgagcga
     1141 tctggcgcac cggacagcgg gccctcgacg tcggcctcga gcatggccgc cgcgatctca
     1201 acagccagcg tgagcgcctc ggcctccccc ccacagagcg ctttcacggt gggatcagcc
     1261 ccgacctggc gctggtcgcc acctacccgc agctcgagta cccgcgccgc tggccggcgg
     1321 gcgtgcgggt gaccgggccg atgatcttcg agcaaccctt tgcggagatc gagctgcccc
     1381 cgggagggga tcccctggtc ctcgtcgccc ccagcaccgc ccacgactcc gacaatcagc
     1441 tcgtgcgcac ggcgctggct gcgctggccg aggagccggt gcgggtggtg gcgacgacca
     1501 accgggtcaa gccccagcag ccgatcgagg tcccggccaa cgccaagctg gtcgattggc
     1561 tcagctacag ccagctgatg ccggccgccg cgctggtgat ctcgcacggc ggccacggca
     1621 ccgtcgcccg cgcgctcggc gccggcaccc cggtcctgat cagcccgatc accggcgaca
     1681 tgagcgagac cgcgatgcgg gtcgactggg ccggcgccgg gctctcgctg ccctggcgcc
     1741 tctgccggcc gacgccgctg cgctgggcgg cgctgcgcct gctgcacgag ccgggcttca
     1801 ccgcgcgggc cgaggcgatc gccgcctggg gccgggagaa cgacggcgcc gtgcgcggcg
     1861 cggagctggt cgagcaactt gccgccgcga aggcgagcgc cctcacctga cgccgccgcg
     1921 ttacgaagct ccgggggtgg gactcgaacc cacaaccctt cgattaacag tcgaatgctc
     1981 tgccgattga gctaccccgg accgctcgcc cggcatgctc cggacgcgtt gaggggaatt
     2041 taccgggtta ccgggtcagg actcgactcg ctgggtgtgg gcgatcttct cgaccagggc
     2101 ggcccgttca cggctcagcg ccgcgcggtg ctcgtagtcg ccgcgctcgc cggcggcggc
     2161 gatctcgtcc tccaagcgcc gctgctccaa gagcaggaag ttcagctcca tcgactcgcg
     2221 cgaggcgggc tggtcgtgcg agagcatcac cagttcggag atcagcgagg ccatctcgtc
     2281 gtcctggggc aggttcgagg cggggtcgcc cgggttctcg cgcagccagg cggcggcgcg
     2341 gatgccgctc gccgagaggt gggcgtcggt gaggcgggcg aggaactcgc ggccatcgtc
     2401 gggcagcgcg atgcacatcg ccagcagcgc ccgctcacgg cgctcgcgcg aggtcagctc
     2461 ggcggcgcgg cgcggctgcc gggccggctg ggactgcgcc tcggcgctga acgactcgta
     2521 ggcctggccc gccgcctgct ctcccccgct cgccggctca gccgcgacca ctcggcccat
     2581 caccgtcgcg ggctcgaagt cgagccgctc ggcgacccgc cggatcaggt cgtcgcgcat
     2641 cgctccctcc tgcattccgg cgaggaccgg cgcgacctct tcgagggcgc ggtcgcgctc
     2701 gaccggcgag gagacgtcgg tgcgggcgag gaccagggca acctggaagg aggtcagctc
     2761 ctcggcccca tcggcgagcg cgcggaagcg ctcggggccg tcgggctcgg ccatcatctc
     2821 ggccgggtcg atcccgtccg gcatcgccgc cacccgcagc cgcatccgcc gcccggcggc
     2881 gacccgctgg gcgcgcagca tcgcctcctg gccggccgag tcggcgtcga gcgccagcac
     2941 cacctcgtcg accatgcccg agagttcctt cacctggtcg tcggtgatcg ccgttcccat
     3001 caccccgacg gcctcctcaa ccccggcctg gtgcagggcg agcacgtcgg tgtagccctc
     3061 gaccacgacc gcgcggcccg ctttcgcgat cgccgctttc gcccggtcga ccccgtagag
     3121 catctggctc ttgtggaaga agtcagtctc ggcggtgttc acgtacttcg ctccctggtc
     3181 ggagcgcatc gcccggccgc cgaagccgag ggtgcggccg cggcggtcgc ggatcgggaa
     3241 catgatccgc gagcggaagc ggtcgtactc gccgccctgg cgcccacgct gggcgaggcc
     3301 gacgccgcgc agctcctcga cgctgaaccc ggcccgctgg ccggcgacga ggatcttgtc
     3361 ccaggcgctg ggcgcgtagc cgaccccgaa gtcgcgcagg acctgctcgc cgagcccgcg
     3421 ctcggccagg tactcgcgcg ccttgccggc ctctttggag tcccagaggt agttggcgta
     3481 gtaggaggcg gtgcggtcga gcagcgcctc gagccggcgc cgctgctggc ggcgcgcctc
     3541 cgcccgcggg tcctctcgct ctcgctccag ctcgaccccg tagcggtcgg cgagcagctc
     3601 gaccgcctcg gcgaagccga gcccctcctt ctcttcgacg aacttgatcg cgtcaccgcc
     3661 gaccccacag ccgaagcagt ggtacagctt ttcctgcgga tcgaccgaga acgagggcgt
     3721 gcgctcctcg tggaacgggc agaggccgac catgcgggtg ccctggcggc gcaggtcggt
     3781 gtgggcggag atcacctcga cgatgtcgac ggtctgcttc agccgatcga ggctctcggg
     3841 cgagatcagg gccatcagaa gcgcgactcc tcgggcacgg tcagctcggt gaacttggcg
     3901 atgcagtagc gatcggtcat cccggcgatg tagtcggcga cccgctggca ggggctggcg
     3961 ccggcatccc actcgggcag ctcctcgggg tgctcgaggt agtgctcgaa gaggccgcgc
     4021 atcgcgcgct ggacgcggtc gtgctcgcgg cgggcgtcgg ggccgagata gacgcgctcg
     4081 aacatgaact tgcgcaggcg cagcatcgcc atcccgatct cctcgctctg gacgatgtcg
     4141 ccggcctcgc gcgagttctc gacgatgtcg atcaccagcg tgtcgatccg cttcgagccg
     4201 gtcgggccga gcagctcgat ctccgccgcc ggcaggtcct cgggacgcag gatcccggct
     4261 cgcagggcgt cgtcgatgtc gtggttgatg taggcgacgc ggtcgaccag cttgacgatc
     4321 cgcccctcca gagtcgccgg cttgttcggc cccgtgtggt tgaggatgcc gtcgcggacc
     4381 tgctcggtga ggttcagccc ttcgccgtcg cgctcgagca cgtcgac
//
LOCUS       DASRIG010000305         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_681342, whole genome shotgun sequence.
ACCESSION   DASRIG010000305 DASRIG010000000
VERSION     DASRIG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681342"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFB52_08305"
     CDS             <1..326
                     /locus_tag="VFB52_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZO06374.1"
                     /translation="MVEKRTGAALVSDGESPEPGIVTERDLLHSIGAGEDPDVELVGE
                     HMSEGVIAATPDWSLERAAAEMSRRNVRHLVVFEGPDAVGVLSMRDIVRVWTSDGATS
                     GMTPD"
     gene            complement(346..639)
                     /locus_tag="VFB52_08310"
     CDS             complement(346..639)
                     /locus_tag="VFB52_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06375.1"
                     /translation="MSEHRHIPEPGETIYVPRPSWAPVFFAIGAVGLVGGIYAAGFIF
                     APYIYAIIGAIFALAAFRSLVRGAIRDYFRLPRKQSVRGAALPVETISAPPKP"
     gene            complement(877..1791)
                     /locus_tag="VFB52_08315"
     CDS             complement(877..1791)
                     /locus_tag="VFB52_08315"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZO06376.1"
                     /translation="MNSGARTAPSPAGAPVPRSAFATALRDYLTLTKPKVQSLLLFTT
                     VTTMYVAGDPSLGLILLTCLGGAMSAGGAGAVNHALDIDIDRMMARTADRPVASGRVS
                     QRAAIGFGVALAAGSFLLMTLTINPLAASLSLSGFLGYVFLYTLWLKRRTWQNIVWGG
                     AAGAVPPLVAWAAVTGELSGMAFYLFAIVFFWTPPHFWALSLLMKDEYAKAGIPMLPV
                     VRGEAETRRQIMLYTVLLYAVTQLPFCAGGLGVAYLVPSMLLGAGFIYFSYRLLRAGD
                     RKWALRTYLFSLAYLALLFLAIAIAANI"
     gene            complement(1797..>3160)
                     /locus_tag="VFB52_08320"
     CDS             complement(1797..>3160)
                     /locus_tag="VFB52_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZO06377.1"
                     /translation="GTALPRLGQIGLGLWAAGGTVLYATLLFTPSEAGVNPLAPLTEL
                     AFLSNDGVDAWASATGLATLGFVLISIDLLTTLRKMRAPGMAWRRVPLFAWAAAVGSW
                     LMLFIGPVLLAALTMLMIDRNYDGVFFADGSGGAPLLWQHLSWIFYSGSYMLILIFAF
                     GAIAEIVTTFSGKPLFNRGVVMGSLAAIAVIGTLAWMQNMLTAPIGIGWMYFAMLMSL
                     ALIVPFGLVMFNLIATMIGGALKMRAPLLFAVGAISMISIGLAAEIGHSMVGAAWNVK
                     NTVDSTAATHFALVGGAVFGGFAALHFWFPKMTGRTMGESLARMSFWTMVVGILLAFV
                     PLFFAGGLEGQVIDAYKFFDGEGVDAYNLIASIGTFVLAIGIVLTLANAIASINGGPE
                     AGHDPWGGDTLEWFTLSPPDPHNFDVLPDVRSDRPMRDIRLAIAQRSARSEDAARESQ
                     PVA"
BASE COUNT          544 a         1009 c         1193 g          414 t
ORIGIN      
        1 tgatggttga gaagcgcacc ggggccgccc tcgtctccga cggcgagtct cctgagcccg
       61 gaatcgtcac cgagcgcgac ctgctccact cgatcggcgc gggcgaggat cccgacgtcg
      121 agctggtcgg cgagcacatg agcgagggcg taatcgccgc gacccccgac tggagcctgg
      181 agcgcgccgc cgccgagatg tcgcggcgaa acgtgcgcca cctggtcgtc ttcgaaggtc
      241 ccgacgcggt tggagtgctc tcgatgcgcg acatcgtccg cgtctggacc tctgacggcg
      301 ctacctcggg gatgacgccg gactgagggc tgctcgggcg aagcgctacg gcttcggggg
      361 cgcgctgatc gtttcgaccg gcagcgccgc tccgcgcacg ctctgcttgc gcgggaggcg
      421 gaagtagtcg cggatcgcgc cgcggaccag ggagcggaag gcggcgaggg cgaagatcgc
      481 gccgatgatc gcgtagatgt agggcgcgaa gatgaagccg gccgcgtaga tgccgccgac
      541 caggccgacc gcgccgatcg cgaagaagac cggggcccag gaggggcggg ggacgtagat
      601 cgtctcgccg ggctcgggga tgtggcggtg ctcgctcatc cctgggcgat gtagaggaag
      661 gcggcgacga aggcgagggc gaagaccgcg gccgcaaagc cgccggcgac caggccggcg
      721 ctcatgttct tgcgtgcagt ctcgcgatcc acagccggat ttctatcagg ttcagagggc
      781 ggcggacctg cgcgctacgg tgtcctcagt cggccaaata gctctgctat tcggcctccc
      841 tgcgtccttg tatcgcttgg tccggcgccc tctgaactaa atgttcgcgg cgatcgcgat
      901 cgccaggaac agcagggcca ggtaggcgag gctgaagagg taggtgcgca gcgcccattt
      961 gcggtcgccg gcgcgcagca ggcggtagga gaagtagatg aagcccgcgc cgagcagcat
     1021 cgagggcacc aggtaggcga cgccgaggcc gccggcgcag aagggcagct gggtcaccgc
     1081 gtagagcagg accgtgtaga gcatgatctg gcgccgcgtc tccgcctcgc cgcggaccac
     1141 cggcagcatc gggatcccgg ccttcgcgta ctcgtccttc atcagcagcg agagcgccca
     1201 gaagtggggc ggggtccaga agaagacgat cgcgaagagg tagaaggcca tgccgctgag
     1261 ctcgccggtc acggcggccc aggcgaccag cggcggcact gcgccggcgg cgccgcccca
     1321 gacgatgttc tgccaggtgc ggcgcttcag ccagagcgtg tagaggaaga cgtagccgag
     1381 gaagccggag agcgagaggc tggccgccag cgggttgatc gtcagcgtca tcagcaggaa
     1441 ggagccggcg gcgagggcga cgccgaagcc gatcgcggcg cgctgggaga cccgccccga
     1501 ggccaccggg cggtcggcgg tgcgggccat catccggtcg atgtcgatgt cgagcgcgtg
     1561 gttgaccgcg ccggcgccgc ccgcggacat cgcgccgccg aggcaggtga gcaggatcag
     1621 ccccagcgag gggtcgccgg ccacgtacat cgtcgtcacc gtggtgaaca gcagcagcga
     1681 ctgcaccttc ggcttggtca gcgtcaggta atcgcggagg gcggtggcga aggccgagcg
     1741 ggggaccggc gcgccagccg ggctgggcgc ggtgcgcgcg ccggaattca tgccggtcag
     1801 gctaccggct gcgactcgcg agccgcgtcc tcgctgcggg cgctgcgctg cgcgatcgcg
     1861 aggcggatgt cacgcatcgg ccggtcgctg cggacgtccg ggaggacgtc gaagttgtgc
     1921 ggatcgggcg gcgagagggt gaaccactcg agcgtgtcgc cgccccaggg gtcgtgcccg
     1981 gcctcggggc cgccgttgat gctggcgatc gcgttggcga gcgtcagcac gatcccgatc
     2041 gccaggacga aggtcccgat cgaggcgatc aggttgtagg cgtcgacgcc ttcgccgtcg
     2101 aagaacttgt aggcgtcgat cacctggccc tcgaggccgc cggcgaagaa cagcggcacg
     2161 aaggcgagca ggatgccgac gaccatcgtc cagaaggaca tccgcgcgag gctctcgccc
     2221 atcgtccggc cggtcatctt cgggaaccag aagtgcagcg ccgcgaagcc gccgaagacg
     2281 gcgccgccga ccagggcgaa gtgggtggcg gcggtcgagt cgaccgtgtt cttcacgttc
     2341 caggcggcgc cgaccatcga gtggccgatc tccgccgcga gcccgatcga gatcatcgag
     2401 atcgccccga ccgcgaacag cagcggcgcc cgcatcttca gcgcgccgcc gatcatggtc
     2461 gcgatcaggt tgaacatgac caggccgaag ggcacgatca gcgccagcga catcagcatc
     2521 gcgaagtaca tccagccgat cccgatcggc gcggtcagca tgttctgcat ccaggcgagc
     2581 gtgccgatca ccgcgatcgc ggccagcgag cccatcacca cgccgcgatt gaacagcggt
     2641 ttgccggaga aggtggtgac gatctcggcg atcgcgccga aggcgaagat caggatcagc
     2701 atgtagctgc cgctgtagaa gatccagctc aggtgctgcc agagcagcgg cgcgccaccg
     2761 gagccgtcgg cgaagaagac gccgtcgtag ttgcggtcga tcatcagcat cgtcagcgcc
     2821 gccagcagga ccgggccgat gaacagcatc agccaggagc cgaccgcggc ggcccaggcg
     2881 aagagcggga cgcggcgcca ggccatgccc ggggcgcgca tcttgcgcag cgtggtcagc
     2941 aggtcgatcg agatcaggac gaagccgagg gtggcgaggc cggtggcgct ggcccaggcg
     3001 tcgacgccgt cgttggagag gaaggcgagc tcggtcagcg gcgccagcgg gttgacgccg
     3061 gcctccgagg gggtgaagag cagggtcgcg tagagcacgg tgccgccggc ggcccagagg
     3121 ccgagaccga tctggccgag ccgcggcagg gcggttccgc
//
LOCUS       DASRIG010000306         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_932881, whole genome shotgun sequence.
ACCESSION   DASRIG010000306 DASRIG010000000
VERSION     DASRIG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932881"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFB52_08325"
     CDS             <1..771
                     /locus_tag="VFB52_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06378.1"
                     /translation="ALLQALEEAPADVSSLRLCLSGGASIARETLDAFQRRFDVRVLE
                     GYGLSETAPACCFSRVDTPAKPGTIGRPLWGVEMRVAGEDGAALPSGEVGEIHVRSHG
                     LMNGYHGRPEATAAAVDPAGWLRTGDLAYVDADGDYFIVDRKKDLILRGGYNVYPREV
                     EEVLYEHPGVLEAAVVGLPDERLGEEVGAALVARPGSELGVDELSAFLDQRVAAYKRP
                     RRFVFLAELPKGPTGKILKRELGESLFAATPADAPASR"
     gene            768..1271
                     /locus_tag="VFB52_08330"
     CDS             768..1271
                     /locus_tag="VFB52_08330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZO06379.1"
                     /translation="MSEHADLARDSGQHFRHVLSHVPTGVVVVAGEDSGGPAGLAVGS
                     FASISLDPPLVGFFCDRGSTSWPLVRRTGGFCVNVLAHNQGEVAARFARKGGAKFAGL
                     PYSSSPHTGSPVLEGVLAWIDCEIEREVELGDHTLVVGATLELAVGDPGHPLVFFRGG
                     YPGLSPD"
     gene            complement(1291..2136)
                     /locus_tag="VFB52_08335"
     CDS             complement(1291..2136)
                     /locus_tag="VFB52_08335"
                     /inference="COORDINATES: protein motif:HMM:NF009467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZO06380.1"
                     /translation="MGALEGKVALISGAARGQGRSHAVRLAEEGADVIALDLCADVGS
                     VAYPLATEADLAETVAAVEATGRRIVSSVTDVRDGAATAAAVAAGVAELGRLDVVVAN
                     AGIVSYFPADELPPQAWKDMIDTNLNGVWNLTSAALRPLIDGGEGGAIVFVSSSAAHV
                     GLAHLAHYSAAKAGLVGLMQSLAIELGPQRIRVNTVHPTAVDTPMVQNEATYALFADG
                     GPVEVTPENPPPAIAEGFGNQHALPTLWVDSADVSNAVVFLTGETGRFVTGTQLRVDA
                     GSAAK"
     gene            complement(2136..2537)
                     /locus_tag="VFB52_08340"
     CDS             complement(2136..2537)
                     /locus_tag="VFB52_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZO06381.1"
                     /translation="MAAAPLKERVLERINAVQDPCSLAQRIPVGLSDMGLVTDVKVSE
                     ADAEGRHDVALVLRVTAPGCMYVPFMDRSIKAAVGDLDEVGEITTEWDPEADWSPGEI
                     AEPARKRIAESRARRLREVRERAKQRSRSRS"
     gene            complement(2547..>3659)
                     /locus_tag="VFB52_08345"
     CDS             complement(2547..>3659)
                     /locus_tag="VFB52_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO06382.1"
                     /translation="VVDGVTHAYEWTPDNAADGLGAEDYHGVLDFFYRHTHLRTEKND
                     PAWMLSFEEFTKKFSAETVAQIYIEETDVDLIVYHEVQIAGFFKNGTSPLQTGEELKR
                     MYPDRVMLYAFADPFNGEAELERMEEKASTGIVDGFKFYPTNGVFDMDTGRMNTMLYD
                     DPELAFPFLQKALDLGVKHVGVHKAFPVAPGPLVKDRPDDICAAAEAFPDLTFEIVHS
                     GWAFLEDCALQLMLNPNVYANLELTANFAVRRPRECAEAIGTMLRVGDVSDRLIFATG
                     IPNGHPQPVLEGLSNMEMPRDLVEGHDMPELTDELKAKILGGNFLAMHGIDPEEKKAQ
                     IAGDEWEQQRNAARERGDDGVHWRPQREAAGTLAAA"
BASE COUNT          465 a         1302 c         1323 g          569 t
ORIGIN      
        1 gcgctgctgc aggcgctcga agaggcgccg gccgacgtct cctcgctgcg cctctgcctc
       61 tccggcggcg cctcgatcgc ccgcgagacg ctcgacgcct tccagcgccg cttcgacgtc
      121 cgcgtgctcg agggctacgg gctctcggag acggcgcccg cctgctgctt ctcgcgcgtc
      181 gacacgccgg ccaagcccgg cacgatcggg cgcccgctct ggggcgtgga gatgcgggtc
      241 gccggcgagg acggcgcggc cctgccgagc ggcgaggtcg gcgagatcca cgtgcgcagc
      301 cacgggctga tgaacggcta ccacgggcgc ccggaggcga ccgccgcggc ggtcgacccg
      361 gcgggctggc tgcgcaccgg cgacctcgcc tacgtcgatg ccgacggcga ctacttcatc
      421 gtcgatcgca agaaggacct gatcctgcgc ggcggctaca acgtctatcc gcgcgaggtc
      481 gaggaggtgc tctacgagca cccgggcgtg ctcgaagccg cggtcgtcgg cctgcccgac
      541 gagcggctcg gcgaagaggt cggcgccgcc ctggtcgccc gccccggcag cgagctcggc
      601 gtcgacgagc tgagcgcctt cctcgaccag cgcgtcgccg cctacaaacg cccgcgccgc
      661 ttcgtcttcc tcgccgagct gcccaagggc ccgaccggaa agatcctcaa gcgcgagctg
      721 ggggagagcc tgttcgcggc gaccccggcc gacgcccccg cgagccggtg agcgagcacg
      781 ccgacctcgc ccgcgactcg ggccagcact tccgccacgt gctctcgcac gtgcccaccg
      841 gggtcgtggt cgtcgccggc gaggactccg gcggaccggc cgggctcgcc gtcggctcct
      901 tcgcttcgat ctcgctcgac ccgccgctgg tcggcttctt ctgcgaccgc ggctcgacca
      961 gctggccgct ggtgcgcagg accggcggct tctgcgtcaa cgtgctcgcc cacaaccagg
     1021 gcgaggtcgc cgcccgcttc gcccgcaagg gcggcgccaa gttcgcgggc ctcccgtact
     1081 cgtcctcacc gcacaccggc tcgccggttc tcgaaggcgt gctcgcctgg atcgactgcg
     1141 agatcgagcg cgaagtcgag ctcggcgacc acaccctcgt ggtcggcgcc acgctcgagc
     1201 tcgccgtcgg cgaccccggc cacccgttgg tcttcttccg cggcggttac ccgggcctga
     1261 gcccggactg agccgcccgc gcagggcgcg ctatttcgcc gcggagccgg cgtcgacgcg
     1321 cagctgcgtg ccggtgacga agcggccggt ctcgccggtc aggaagacga ccgcgttgct
     1381 gacgtcggcc gagtcgaccc agagggtcgg cagcgcgtgc tggttgccga acccttcggc
     1441 gatcgccggg ggtgggttct cgggggtcac ttcgaccggc ccgccgtcgg cgaacagcgc
     1501 gtaggtcgcc tcgttctgga ccatcggcgt gtcgaccgcg gtcgggtgca cggtgttgac
     1561 ccggatccgc tgcggcccga gctcgatcgc gaggctctgc atcagcccga ccaggccggc
     1621 cttggcggcg gagtagtggg cgaggtgggc gaggccgacg tgggcggcgc tggagctgac
     1681 gaagacgatc gcgccgccct cgccgccgtc gatcagcggc cgcagcgcgg ccgaggtgag
     1741 gttccagacg ccgttcaggt tggtgtcgat catgtccttc caggcctgcg gcggcagctc
     1801 gtcggcgggg aagtaggaga cgatcccggc gttggcgacg accacgtcga gccggccgag
     1861 ctcggcgact cccgccgcga ccgccgccgc ggtggcggcg ccgtcgcgga cgtcggtgac
     1921 cgagctgacg atccgccggc cggtcgcctc gaccgccgcg accgtctcgg ccaggtcggc
     1981 ctcggtggcg agggggtagg cgaccgagcc gacgtccgcg cagaggtcga gggcgatgac
     2041 gtcggcgccc tcctcggcga gccgcacggc gtgcgagcgg ccctggccgc gggcggcgcc
     2101 gctgatcagc gcgaccttgc cctcgagcgc gcccatcagc tgcggctccg gctgcgctgc
     2161 ttggcccgct cgcgcacctc gcgcaggcgc cgggcgcgcg actcggcgat ccgcttgcgg
     2221 gccggctcgg cgatctcgcc ggggctccag tccgcctccg ggtcccactc ggtggtgatc
     2281 tcgccgacct cgtcgaggtc gccaaccgcg gccttgatcg agcggtccat gaacggcacg
     2341 tacatgcagc cgggggcggt cacgcgcagg accagggcga cgtcgtggcg gccctcggcg
     2401 tcagcctcgg agaccttcac gtcggtgacc aggcccatgt cggagagccc gaccgggatc
     2461 cgctgggcga ggctgcaggg atcctggacc gcgttgatcc gctccaggac ccgctctttg
     2521 aggggcgcgg cggccatcgt cccgacctaa gccgcggcga gcgtcccggc ggcttcgcgc
     2581 tggggacgcc agtggacgcc gtcgtcgccg cgctcgcggg ccgcgttgcg ctgctgctcc
     2641 cactcgtcgc cggcgatctg cgccttcttc tcctcggggt cgatgccgtg catcgcgagg
     2701 aagttgccgc cgaggatctt cgccttcagc tcgtcggtca gctccggcat gtcgtgtccc
     2761 tcgaccagat cccgcggcat ctccatattg gacaggccct cgagcaccgg ctgcgggtgg
     2821 ccgttgggga tcccggtggc gaagatcaac cggtcggaga cgtcgccgac ccgcagcatc
     2881 gtgccgatcg cttccgcgca ctcgcgcggg cggcggacgg cgaagttggc ggtgagctcc
     2941 aggtttgcgt agacgttcgg gttcagcatc agctgcaggg cgcagtcctc gaggaaggcc
     3001 cagccgctgt gcacgatctc gaaggtgagg tcgggaaagg cctcggcggc ggcgcagatg
     3061 tcgtcgggcc gatccttgac cagcgggccg ggggcgaccg ggaaggcctt gtgcacgccg
     3121 acgtgtttga ccccgaggtc gagcgccttc tgcaggaagg gaaaggccag ctccgggtcg
     3181 tcgtagagca tcgtgttcat ccggcccgtg tccatgtcga agacgccgtt ggtcgggtag
     3241 aacttgaagc cgtcgacgat gccggtcgac gccttctcct ccatccgctc cagctcggcc
     3301 tcgccgttga aggggtcggc gaaggcgtag agcatcaccc ggtccgggta catccgcttc
     3361 agttcttcgc cggtctgcag cggtgaggtg ccgttcttga agaagccggc gatctggacc
     3421 tcgtggtaga cgatcaggtc gacgtcggtc tcctcgatgt agatctgggc gaccgtctca
     3481 gcgctgaact tcttcgtgaa ctcctcgaag ctgagcatcc acgcggggtc gttcttttcc
     3541 gtgcgcaggt gggtgtggcg gtagaagaag tcgaggacgc cgtggtaatc ctcggcgccg
     3601 agaccgtcgg cggcgttatc cggggtccat tcgtaggcgt gcgtgactcc gtcgacgac
//
LOCUS       DASRIG010000307         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3544769, whole genome shotgun sequence.
ACCESSION   DASRIG010000307 DASRIG010000000
VERSION     DASRIG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544769"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFB52_08350"
     CDS             <1..873
                     /locus_tag="VFB52_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZO06383.1"
                     /translation="RRALHPLIVATNAPANWVVRVFFRVDADSVAEETSPEELRLLIS
                     RGAQRGALDTGEAGHLAGVFHLHEQEAREVMTPIPAVVTVDASETVEAALRRCVTSGH
                     TRLVVIEDDNPDRVKGIVHNNSLARLYMSDGPDASIEPLVREALIVPETRPLDDLLQD
                     LQVQRTSLAVVVDEYGRTVGIVTVEDILEEVVGEIEDETDPRAAALRQLTNGDWFVRG
                     HVSLGDLADAGIELPADTDAYNSVGGYVFGELGRLPKRGDSIAANGYTIRVEAVRENR
                     IEAVRISRSVRPRA"
     gene            919..1164
                     /locus_tag="VFB52_08355"
     CDS             919..1164
                     /locus_tag="VFB52_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06384.1"
                     /translation="MWVVRLTDKQLEVLRALLRSEEHLGPALKGSIEALDLARWDELP
                     EAELPWEEIESAARRQGISEADVIWDISGKRKAASAE"
     gene            complement(1166..1987)
                     /locus_tag="VFB52_08360"
     CDS             complement(1166..1987)
                     /locus_tag="VFB52_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06385.1"
                     /translation="MSSNQGYESPGIAGSGAIATGLAAVSTTTADTILLARSEASAWR
                     AEEKIVAACAKIEGAEPSRLRVTTDPADLAECDVVVEAIVEEVAPKGELLARVAAACP
                     NADLATTTSSLSIGEIAAAAGTDKLYGLHVFNPVPRMALVEVCVPSGDAGVRERALAW
                     CAALGKTAVEVPDQAGFVVNRLLFPYLFEAARLMERTGMEAGEVDTCMKLGAGHPMGP
                     LALLDFVGLDVSKAIGDALYADNGELASQVPGLIIEKVGKGELGRKSGEGFYTYD"
     gene            2146..2808
                     /locus_tag="VFB52_08365"
     CDS             2146..2808
                     /locus_tag="VFB52_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06386.1"
                     /translation="MERLKITLVGLVLVLALVVAYGYGCGDSGDDSGDGGDSVATEAD
                     STAGDGTGASTDGEGGDSSEATDSTESTDGGNSTEGGDSSSGDSDSGSTSSSGKPLTK
                     AAYIEQGDLICQGVPQKYGELVQELEQEAKKQKKPKPNTAEINLAAAVPPLPEAAKEL
                     EELVPPKGDEAEAEAIVAALEAAAKGLEEKPNSELSGPKSPFDEFQKLTGKYGFRACS
                     QL"
     gene            2864..>3911
                     /locus_tag="VFB52_08370"
     CDS             2864..>3911
                     /locus_tag="VFB52_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06387.1"
                     /translation="MDLTLSPSEEEFRDEVRTWIQESNPGPEPEGSLDEVMAFRGEWQ
                     RKLHAAGWAGISWPKEYGGRGATMIEQAIFVGEAARQEAPSPANVLGLAMGGPVVIAH
                     GTEAQKERYLEPILTGEEIWCQGFSEPESGSDLASLKTRAVKDGDEWVVTGQKVWTTF
                     AQYAKWCMLVARTDPDAPKHQGLTYFLMDMEQEGVEAKPLVQITGEGEFNEVFINEAR
                     VPEENIVGSVGEGWGVAITTLMNERAGLAFGAIAQIQNSLGRLARLAREVRVEGGGSA
                     ADDTYYRQRIAQLFIEAETMRLNAYRGLTKTMQSGIPGPEGSLGKWQWADINQDLTEL
                     ALEIEGAYAALGRGS"
BASE COUNT          694 a         1291 c         1352 g          574 t
ORIGIN      
        1 cgccgcgccc tgcacccgct gatcgtcgcc accaacgcgc cggccaactg ggtggtccgc
       61 gtcttcttcc gggtcgatgc cgactcggtc gccgaggaga cctcgccgga ggagctgcgc
      121 ctgctgatct cgcgcggcgc ccagcgcggc gccctcgaca ccggcgaggc cgggcacctg
      181 gccggcgtct tccacctgca cgagcaggag gcccgtgagg tgatgacccc gatcccggcc
      241 gtggtcacgg ttgacgcctc ggagacggtt gaggcggcgc tgcgccgctg cgtcacctcc
      301 ggccacacgc gcctggtcgt gatcgaggac gacaaccccg accgggtcaa ggggatcgtc
      361 cacaacaaca gcctcgcccg gctctacatg agcgacgggc ccgacgcctc gatcgagccg
      421 ctggtccgcg aagcgctgat cgtccccgag acgcggccgc tcgacgacct gctgcaggac
      481 ctccaggtcc agcgcacctc gctcgcggtc gtcgtcgacg agtacgggcg cacggtcggg
      541 atcgtcacgg tcgaggacat cctcgaggag gtggtgggcg agatcgagga cgagaccgat
      601 ccccgcgccg ccgccctgcg ccagctcacc aacggcgact ggttcgtgcg cggccacgtc
      661 tcgctcggcg acctcgccga cgccgggatc gagctgccgg ccgacaccga tgcctacaac
      721 tcggtcggcg gttacgtctt cggtgagctc gggcggctgc ccaagcgcgg cgactcgatc
      781 gccgccaacg gctacacgat ccgggtcgag gcggttcgcg agaaccggat cgaggcggtg
      841 cggatcagcc gttccgtccg tccgcgcgcc tagtttctcc gagcaacccg cgacccgccg
      901 aacagttgga ggtaccgcat gtgggttgta cgcctgaccg acaagcagct ggaggtcctg
      961 agggcgctac tgcgctccga ggagcatctc ggcccggcgc tcaaaggctc gatcgaggcc
     1021 ctcgacctcg cccgctggga cgagctgcct gaagccgagc tcccttggga ggagatcgag
     1081 tccgccgccc gccgccaagg catttccgag gccgatgtga tctgggacat cagcggaaaa
     1141 cgcaaggcgg cgagcgccga gtaactcagt cgtacgtgta aaagccctcg ccgctcttgc
     1201 ggcccagttc gcccttgccg accttctcga tgatcaggcc gggcacctgg ctggcgagct
     1261 cgccgttgtc ggcgtagagg gcgtcgccga tcgctttcga gacgtcgagc ccgacgaagt
     1321 cgagcagcgc cagcggcccc atcgggtgcc cggcgccaag cttcatgcag gtgtcgacct
     1381 cgcccgcctc catgccggtg cgctccatca gccgcgcggc ttcgaacagg taggggaaga
     1441 gcagccggtt gacgacgaaa cccgcttgat cgggaacctc gaccgcggtc ttgccgagcg
     1501 ccgcgcacca ggcgagcgcc cgctcgcgga ccccggcgtc gccgctgggg acgcagacct
     1561 cgaccagtgc catccgcggc accgggttga agacgtgcag gccgtagagc ttgtcggttc
     1621 ccgccgccgc ggcgatctcg ccgatgctga gcgaggaggt ggtcgttgcc agatcggcgt
     1681 tcgggcaggc cgcggcgact cgcgccagca gctcaccctt cggcgcgacc tcctcgacga
     1741 tcgcctcgac gacgacgtcg cactcggcga ggtcggcggg atcggtcgtg acccgcagcc
     1801 gcgagggctc cgccccctcg atcttcgcgc aggcagcgac gatcttctcc tcggcgcgcc
     1861 aggccgaggc ctcgctgcgg gcaagcagaa tcgtgtctgc ggtggtggtc gagacggcgg
     1921 cgaggccggt ggcaatagcc ccgctccccg cgatccccgg actttcgtac ccctggttcg
     1981 agctcattgg cggggtttct aacacgctgt catcagatcg tgtcgcgtga cagcggccaa
     2041 cttaaagatt ctccgaaaac cagccgcccg ctcttgactt gaacttcttc gcctgtaggt
     2101 tctccgacta atcaagtttg tcgtgctatt gcaggggaag gggacatgga gcggctgaag
     2161 atcactctcg tcgggctggt gttggtgctc gcgctcgtgg ttgcgtacgg ctacggctgc
     2221 ggggacagcg gtgacgacag cggcgacggt ggtgattcag tcgcgaccga agcggacagc
     2281 acggcaggcg acggcacggg cgcgtcgacc gacggagaag gtggcgacag ctctgaagcg
     2341 acggacagca ccgaatcaac ggatggaggg aactcgaccg aaggcggcga ctcgtcgtcg
     2401 ggtgactcgg actccggcag cacctcttcc agcggcaagc cactgaccaa agcggcctac
     2461 atcgaacaag gtgacctgat ctgccaggga gtcccgcaga aatacgggga actcgtccag
     2521 gaactggaac aggaagcgaa aaaacagaag aagccaaaac caaataccgc cgaaatcaat
     2581 ctggcggccg cggttccgcc actaccggaa gcggcgaagg aactggaaga actggtgccc
     2641 ccgaaaggtg acgaggcaga ggccgaagcg atcgtcgcag ccctcgaagc ggcggcgaaa
     2701 gggctcgaag aaaagccaaa ctcggaactt tccggtccca aatcgccatt cgacgaattt
     2761 caaaaactga cgggcaaata cggcttccgg gcttgtagcc agctctagcc gccagttcgg
     2821 atccgactgg ccgttcggcc aagcgctata tgctcagccc tcgatggacc tgacgctgtc
     2881 gccatcggag gaggagttcc gcgacgaggt tcgcacctgg attcaggaga gcaaccccgg
     2941 tccggaaccg gaaggcagcc tcgacgaggt gatggccttc cggggcgagt ggcagcgcaa
     3001 gctgcacgcc gccgggtggg ccgggatctc ctggcccaag gagtacggcg gccgcggcgc
     3061 gacgatgatc gagcaggcga tcttcgtcgg cgaggcggcg cgccaggaag cgcccagccc
     3121 ggccaacgtg ctcgggctcg cgatgggtgg gccggtggtg atcgcgcacg ggaccgaggc
     3181 gcagaaggag cgctacctgg agccgatttt gaccggcgag gagatctggt gtcagggctt
     3241 ctccgagccc gagtccggat ctgacctggc ctcgctgaag acccgtgcgg tcaaggacgg
     3301 cgacgaatgg gtggtgaccg ggcagaaggt ctggacgacc ttcgcccagt acgcgaagtg
     3361 gtgcatgctg gttgcccgca ccgaccccga cgcgcccaag caccagggcc tcacctactt
     3421 cctgatggac atggagcagg agggcgtgga agcgaaaccg ctggtccaga tcaccggcga
     3481 gggcgagttc aacgaggtct tcatcaacga ggcgcgggtc cccgaggaga acatcgtcgg
     3541 cagcgttggc gagggctggg gggtggcgat aacgacgctg atgaacgagc gcgccgggct
     3601 tgccttcggc gcgatcgcgc agatccagaa cagcctcggc cggctcgcca ggctggcccg
     3661 cgaggtgcgc gtggaggggg gcggatcggc cgccgacgac acctactacc gccagcggat
     3721 cgcgcagctc ttcatcgagg ccgagacgat gcggctgaac gcctaccgcg gcctgaccaa
     3781 gacgatgcag tcgggcatcc ccgggccgga gggctcgctg ggcaagtggc agtgggccga
     3841 catcaaccag gatctgaccg agctggcgct ggagatcgag ggcgcctacg cggcgctcgg
     3901 ccgcggttcc g
//
LOCUS       DASRIG010000308         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1208300, whole genome shotgun sequence.
ACCESSION   DASRIG010000308 DASRIG010000000
VERSION     DASRIG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208300"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VFB52_08375"
     CDS             complement(<1..1659)
                     /locus_tag="VFB52_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06388.1"
                     /translation="MRSAILGFTIALAALLAAAPGAGAKGQESVTLVWLPEGTTVAQI
                     ADAGLSPGLLSAGLGSVPAEQTYLDVSQGNRVFNSLYDEPLPVRAGNPDWWAEVVERA
                     ESAPAEIVPGLLERTLTEAGRREALQTRSVRVGELPALVEELEGDDLLIAIAKPPPAE
                     NRQLAVGVAGRGFDGNLTSDSTRADGYVLSTDVAPTVLDRLGIAIPGEMSGQPIRSED
                     ELDVGEVEALGDRMAVISHRRGPVIGFSLLAWLLTLGLVAAASRGALARPAVRVTALS
                     VVYMPLAMLAGAAIEPGETAEVLLVMLGAPLLAALTLVFLRGFRAIAIAAALTVGAYA
                     LDLVLGSPLTSLSLLGPNPGLGVRFYGIGNELEALLAVLVVAGTGTALAGFWPQLRRN
                     RAAIAFLLVGLLAAFVFAAGRFGADVGAAIVFPLGAAVAAVAVAGLGRRRALLAIIAA
                     PFAVLALLALVDLVSGANAHLTRSVLDAGGLGDLSDVAQRRLQLSANSFGRPVLLVFL
                     PVVAILAGLAIWRRDRLREWVRDVPAVRAGLLGALAATAVGTLAN"
     gene            1646..2341
                     /locus_tag="VFB52_08380"
     CDS             1646..2341
                     /locus_tag="VFB52_08380"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZO06389.1"
                     /translation="MADRTFAKFTFFKMDPAWRRRDPELRAADKQEFLAACEDFGADR
                     SLRAYSTIGTRGDVDLVLLSQSANLQNIHDFHVVLGQSGLAQWAQTPYSFLSMTKPSP
                     YSEEEARPEICTSERKYLFLYPMVKQRRWYGLPPQERGRIMKSHIEVGRRYPEITINT
                     TYSYGIDDQEFVVCFEGDDPGMFLDLVNELRPTESSEYTELETPIFTCVAMSVRKALD
                     ALDGTPSAIAAGA"
BASE COUNT          403 a          848 c          809 g          290 t
ORIGIN      
        1 gttggcgagc gtgccgaccg cggtggcggc gagcgccccg agcaggcccg cgcgcactgc
       61 cgggacgtcg cgaacccact cccgaagccg atcgcggcgc cagatcgcca ggccggcgag
      121 gatcgcgacg accggcagga agaccagcag caccgggcgg ccgaagctgt tggcggagag
      181 ctgcagacgg cgctgggcga cgtcggagag gtcgccgaga ccgccggcat cgagcaccga
      241 gcgggtcagg tgggcgttgg ccccggagac gagatcaacg agcgccagca gcgccaggac
      301 cgcgaacggc gcggcgatga tcgcgagcag ggcgcggcga cggccgaggc cggcgacggc
      361 gaccgcggcg acggcggcgc cgagtgggaa gacgatcgcc gcgccgacgt cggcgccaaa
      421 gcgtccggcc gcgaagacga aggccgcaag gaggccgacg agaaggaagg cgatcgcggc
      481 ccggttgcgc cggagctgtg gccagaaccc cgccaacgcc gtgccggtgc cggcgacgac
      541 cagcaccgcg agcagcgcct cgagctcgtt gccgatcccg tagaagcgca cgcctagccc
      601 tgggttgggg ccgagcagcg agagcgaggt gagcggggaa ccgagcacca ggtcgagcgc
      661 gtaggcgccg acggtcaggg cggcagcgat ggcgatcgcg cgaaacccgc gcaggaacac
      721 cagggtcaat gccgccagca ggggcgcgcc cagcatcacc agcagcactt ccgccgtctc
      781 ccccggctcg atcgcggcgc cggcgagcat ggccagcggc atgtagacga cggagagcgc
      841 ggtgacgcgg accgccggcc gggccagcgc cccgcggctg gccgcggcga cgagcccgag
      901 ggtgagcagc caagccagca agctgaagcc gatcacgggc ccgcgccggt gcgagatcac
      961 cgccatccgg tcgccgagcg cttctacctc acctacgtcg agttcgtcct cgctgcggat
     1021 cggctgtccc gacatctccc ccgggatcgc aatccctagc cggtcgagca cggtcggcgc
     1081 gacgtcggtg ctgagcacgt aaccgtcggc gcgggtgctg tcggaggtga ggttgccgtc
     1141 gaaccctcgg ccggcgacgc caacggccag ctgccgattc tccgccggag gcggcttcgc
     1201 gatcgcgatc aggagatcgt ctccctcaag ctcctctacg agcgcgggca actcccccac
     1261 cctcaccgag cgcgtctgga gcgcctcacg gcgcccagct tccgtcagcg tccgctccag
     1321 caacccgggc acgatctccg ccggcgccga ttcggcccgt tcgacgactt ccgcccacca
     1381 gtccgggttg cccgcgcgca ccggcagtgg ctcgtcatat agagagttga agacccggtt
     1441 tccctgcgag acgtcgagat aggtctgctc tgccggcacg gacccgagcc ctgccgacag
     1501 cagtcctggc gagagccccg cgtcggcgat ctgcgcgacc gtggtccctt cggggagcca
     1561 gacgagcgtg acgctctcct ggcccttcgc ccccgccccc ggagctgcgg ccagcagcgc
     1621 ggcgagcgct atcgtgaatc ccagaatggc cgaccgcacg ttcgcgaagt tcacgttttt
     1681 caagatggac ccagcctggc gccggcgcga tcccgagctg cgggccgccg acaagcagga
     1741 attcctcgcc gcctgcgagg acttcggagc agaccgctcg ttgcgcgctt actcaaccat
     1801 aggcacgcgc ggcgacgtcg acctggtcct gctcagccag agcgccaacc tgcagaacat
     1861 ccacgacttc cacgtcgtgc tcgggcaaag cgggctcgcg cagtgggcgc agacgcccta
     1921 ctcgttcctc tcgatgacca aaccctcgcc ctactccgag gaggaggcgc ggccggagat
     1981 ctgcacctcc gagcgcaagt acctctttct ctacccgatg gtcaagcagc gccgctggta
     2041 cggcctgccg ccgcaggagc gcggccggat catgaaaagc cacatcgagg tcggccgccg
     2101 ctaccccgag atcacgatca acacgaccta ctcctacggg atcgacgacc aggagttcgt
     2161 cgtctgcttc gagggcgacg atccgggcat gttcctcgac ctggtcaacg agctgcgccc
     2221 gaccgagtcg agcgagtaca ccgagctgga gacgccgatc ttcacctgcg tggcaatgtc
     2281 ggtgcgcaag gcgctcgatg cgctcgacgg cacgccgagc gctatcgccg ccggcgcatg
     2341 agcaccaccg
//
LOCUS       DASRIG010000309         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_934646, whole genome shotgun sequence.
ACCESSION   DASRIG010000309 DASRIG010000000
VERSION     DASRIG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934646"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFB52_08385"
     CDS             <1..367
                     /locus_tag="VFB52_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06390.1"
                     /translation="DEEGERGVCVNTASIAAYEGQVGQVAYAASKGGVVGLTLPVARE
                     LAPKAVRVVTIAPGLFETPMMAGLPEAARESLGGTVPYPPRLGDPAEYADLVEHLVTN
                     AMINGETIRLDGALRMAPR"
     gene            364..1335
                     /locus_tag="VFB52_08390"
     CDS             364..1335
                     /locus_tag="VFB52_08390"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZO06391.1"
                     /translation="MIATRFTETFGIEHPVMQGGMQWVGRAELVAAVANAGALGTITA
                     LTQPTPEALSDEIARCRELTDKPFGVNLTILPSINPPPYAEYRQAIIDAGVKIVETAG
                     SNPQEHLEHFHPAGVKVIHKATSVRHALKAQSLGVDAVSIDGFECAGHPGEDDVPGLV
                     LIPAAASVIEIPMIASGGIADGRGLIAALALGADGVNMGTRFMCTAESPIHQRIKEAI
                     VANDERSTNVIFRPFRNTARVVRNSVSDEVLDIQDDGGQFEDVRHLVSGKRGATVYET
                     GDPEAGIWWAGQVQGLIGDVPTCAELLERIVGEAEEIAGRRLPAMLG"
     gene            complement(1350..2123)
                     /locus_tag="VFB52_08395"
     CDS             complement(1350..2123)
                     /locus_tag="VFB52_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZO06392.1"
                     /translation="MCFQFDASPPELPADRLLPIAGGAAAEQLELRSADGTAFAAALA
                     ESPQPSGTAIAILPDIRGLYRFYVELAERFAAAGHHAIAIDYFGRTAGVAERAEDFDH
                     MSHVAAVVPENVQADLAPAIARVLECPGAERVVSVGFCFGGAQSFLAATNADLGLDGA
                     VGFYGTLDPSKFGIENMPFPLRHGTETKVRVLGLFGGADDFIPPADIETFDGQLAEAG
                     VEHELITYDGCPHSFFDRAQEQFADQSADAWRRVLDFVA"
     gene            2355..3497
                     /locus_tag="VFB52_08400"
     CDS             2355..3497
                     /locus_tag="VFB52_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO06393.1"
                     /translation="MLVALAALCLAVGLAACGDSDDDSTGSSGAETTAESTGETGGSD
                     IQAEAAALVAEDEEPPAEVPEALPLKDTNIPKGKTVAFLGCGAAQCETYFPPIQKGAE
                     ALGWTAKMYQGSVEPNKQVGFLEEIVKSDPDVFIACCIAPSLGKKYFNQMKENGTITF
                     MCCTAEPGTEGIDKLVSLPSDKLGSGEALANFALAEKGEELSTLYVTTPDFEVSAEYF
                     EGYENQIKKLCSECSVDSIEVATEDIGKPALTTAVTGYLQSHPDVNFVGALFSPLLIG
                     VSAAMDAAGIEEVSLGATANDELALNAITEGKEHWVAVQNFGQEFGLWGINFAVRTIQ
                     GEEIGDETLTPEVLVTNKNAEKVLAHPEQWVIPDALEQYEALWGLK"
BASE COUNT          646 a         1126 c         1225 g          504 t
ORIGIN      
        1 cgacgaggag ggcgagcggg gcgtctgcgt caacacggcc tcgatcgccg cctacgaggg
       61 ccaggtcggc caggtcgcct acgccgcctc gaagggcggg gtcgtcggcc tcaccctgcc
      121 ggtcgcccgc gagctggcgc cgaaggcggt gcgcgtggtg acgatcgcgc cgggcctgtt
      181 cgagacgccg atgatggccg ggctgccgga ggcggcgcgc gagtcgctcg gcggcaccgt
      241 gccctacccg ccgcggctcg gcgacccggc cgagtacgcc gacctggtcg agcacctggt
      301 caccaacgcg atgatcaacg gcgagacgat ccggctcgac ggcgcgctgc ggatggcgcc
      361 gcgatgatcg cgacgcgctt caccgagacc ttcggcatcg agcacccggt gatgcagggg
      421 gggatgcagt gggtggggcg ggcggagctg gttgccgcgg tcgccaatgc cggcgcgctc
      481 ggcacgatca ccgcgctgac ccagccgacg ccggaggcgc tgagcgacga gatcgcccgc
      541 tgccgcgagc tcaccgacaa gccgttcggg gtcaacctga cgatcctgcc gtcgatcaac
      601 ccgccgccct acgccgagta tcgccaggcg atcatcgacg ccggggtgaa gatcgtcgag
      661 accgcgggct cgaacccgca ggagcacctc gagcacttcc accccgccgg ggtcaaggtg
      721 atccacaagg cgacctcggt ccggcacgcg ctgaaagcgc agtcgctggg cgtcgacgcg
      781 gtctcgatcg acggcttcga gtgcgccggt caccccggcg aggacgacgt ccccgggctg
      841 gtcctgatcc cggcggcggc gagcgtgatc gagatcccga tgatcgcctc cggcgggatc
      901 gccgacggcc gcggtctaat cgcggcgctg gcgctcggcg ccgacggggt caacatgggc
      961 acccgcttca tgtgcacggc ggagtcgccg atccaccagc ggatcaagga ggcgatcgtc
     1021 gccaacgacg agcgctcgac caacgtgatc ttccgcccct tccgcaacac cgcgcgggtg
     1081 gtccggaact cggtctccga cgaggtcctg gacatccagg acgacggcgg ccagttcgag
     1141 gacgtccgcc atctcgtctc gggcaaacgc ggcgccaccg tctacgagac cggcgacccc
     1201 gaggcgggga tctggtgggc gggacaggtc cagggcctga tcggcgacgt cccgacctgc
     1261 gccgagctgc tcgagcggat cgtcggcgaa gccgaggaga tcgccggcag gcgcctcccc
     1321 gcgatgctgg gctgaggaac ggcctgtccc taggcgacga agtcgagcac tcggcgccag
     1381 gcgtcggcgg actggtcggc gaactgctcc tgggcgcggt cgaagaagga gtgggggcag
     1441 ccgtcgtagg tgatcagctc gtgctcgacg ccggcctccg ccagctggcc gtcgaaggtc
     1501 tcgatgtcgg ccggcgggat gaagtcgtcg gcgccgccga agaggccgag cacgcggacc
     1561 ttggtctcgg tgccgtgacg gagcgggaag ggcatgttct cgatcccgaa cttggagggg
     1621 tcgagggtgc cgtagaagcc gaccgcgccg tcgagcccga ggtcggcgtt ggtggcggcg
     1681 aggaacgact gggcgccgcc gaagcagaag ccgacgctga cgacgcgctc cgcgcctggg
     1741 cactcgagca cgcgggcgat cgccggggcg aggtcggcct gcacgttctc ggggacgacc
     1801 gcggcgacgt gggacatgtg gtcgaagtcc tcagcccgct ccgcgacccc ggcggtgcgg
     1861 ccgaagtagt cgatcgcgat cgcgtggtgg ccggcggcgg cgaagcgctc ggccagctcg
     1921 acgtagaagc ggtagaggcc gcggatgtcg ggcaggatcg cgatcgcggt gccgctcggc
     1981 tgcggcgatt cggcgagcgc ggcggcgaag gcggtgccgt cggcggagcg cagctccagc
     2041 tgctcggccg cggctccccc tgcaattggc agcaaacggt cggccggcag ctccggcggg
     2101 ctggcatcga actggaagca catctgtggt ccctcacttt cctggcgtaa ccccggaggc
     2161 ctgcttcgcg gtctgcgttg caggttaatc atcaagatga tataaatttt ctgcctcgca
     2221 tcggtatggg cgagctggag gagaaagcga caggagcctg ctgggagcgg ctctgaggac
     2281 ggaaagtcgc gatcccagtg gagaaacact cagttggagg agagaaacga atgaagaatc
     2341 agaggttccg atggttgctc gtcgcgctcg ccgcgctctg ccttgccgtt gggctcgcag
     2401 cctgcggcga tagcgacgac gacagcacgg gctcgagcgg agccgaaacg accgccgaat
     2461 cgaccggcga aaccggcggc tcggacatcc aggcggaagc ggccgcgctt gtcgcggaag
     2521 acgaagaacc gcccgctgag gtgccggaag cgctgccgct gaaagacacc aacatcccga
     2581 agggcaaaac cgttgccttc ctcggctgtg gtgccgctca gtgcgaaacg tacttcccgc
     2641 cgatccagaa aggcgcggag gcgctcggat ggacggcaaa gatgtaccag ggcagcgtcg
     2701 aaccgaacaa acaggtcggg ttcctcgaag aaatcgtcaa atccgacccc gacgtgttca
     2761 tcgcctgctg catcgcgccg tctctgggga aaaaatattt caaccagatg aaagaaaacg
     2821 gcacgatcac cttcatgtgc tgcacggctg agccgggcac cgaagggatc gacaagctgg
     2881 tctcgctccc ctcggacaag cttggctctg gtgaagctct ggccaacttc gcgctcgccg
     2941 aaaaaggcga agaactgagc acgctgtatg tcaccactcc ggacttcgaa gtcagtgcgg
     3001 agtacttcga aggctacgag aaccaaataa agaaactctg ttcggagtgc tcggtcgact
     3061 cgatcgaagt cgcgaccgaa gacatcggca aaccggcgct cacgaccgca gtgacgggct
     3121 acctgcagtc tcatcccgac gtcaacttcg tcggggcgct gttctcgccg ctgctgatcg
     3181 gagtttcggc ggcgatggat gccgcgggaa tcgaagaagt cagtctcggt gcgacggcca
     3241 acgacgaact cgcgctcaac gccatcaccg aaggcaaaga acactgggtt gccgtccaga
     3301 acttcggtca ggaattcggg ctgtggggca tcaacttcgc cgtccgcacg attcagggcg
     3361 aagaaatcgg tgacgaaacc ctcacccccg aagtcctcgt cacgaacaaa aacgccgaaa
     3421 aagtgctcgc ccacccggaa cagtgggtga tcccggacgc actcgagcag tacgaagccc
     3481 tctggggcct caaatagcaa g
//
LOCUS       DASRIG010000310         7567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_684494, whole genome shotgun sequence.
ACCESSION   DASRIG010000310 DASRIG010000000
VERSION     DASRIG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684494"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFB52_08405"
     CDS             <1..457
                     /locus_tag="VFB52_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06394.1"
                     /translation="LVLADLAELGLRRAVRVGTATGLDPELEAGELVVVSEAHAWGGP
                     GGGEGAAVLPDAELAEALARQLGIAARPGSVASLDTLHGQLDEVVAASLGSDVADMQT
                     AALLTRGRELELAVAAVLVVSETGAGEKLDDEPLELAAKRAGTAAAAVL"
     gene            complement(473..889)
                     /locus_tag="VFB52_08410"
     CDS             complement(473..889)
                     /locus_tag="VFB52_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06395.1"
                     /translation="MNEMQKGTGGVMDEFHDQDGQQYSQDSLRARSEQALGELAQALI
                     DNPTLHNAVTAALGAREKAIEAQRAAMSALDIPSASDLERVERRLRSFSQRLEEVEEQ
                     IDDLSREVGALRRQLAKQDDEQSEKKEKKAPKKAKA"
     gene            1098..3569
                     /locus_tag="VFB52_08415"
     CDS             1098..3569
                     /locus_tag="VFB52_08415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZO06396.1"
                     /translation="MRTKIALIVALASFLAATALSAAVAPADSGTGSVIGGTPLAPIA
                     PAASPTAPACSNQIDDDGDGLVDLDDSACTEPAWTSEEAAPAETTAPVPTPAAPPAEG
                     DDKDKGEKDSSKDGVKQGDSIGGDAAKHKRGGATVNEPLGDTSGGRKSDGAIEAPSAT
                     GNGDQPLDSDGNGGAQFNDGGSPTLGNPTTTIAPFGPAPIGVPNFIIDSFEIPPFLLP
                     IYQACGTEYGVPWEVLASINKIETGFGTNLNVSTAGAIGWMQFLPSSWEAFGVDANGD
                     RKKDPYNPVDAICAAANYLEIAGAKENLYDAIFAYNHADWYVQEVLLYARAYGRLPSD
                     LVGSLTGLTEGAHFPIAADASYADDVAARASLKSGDYTAAGSAAEVISNSPTERGINI
                     FADEGAPVVAVNDGVVRKTGHSEKLGNFVVLEDTYGNRYTYAALGELVRDERLVVMPS
                     GEEKRLPVESENLRPRLRALPGRIEDDGLGHAEGKPSGGNSAGELQPDDVIGSGASEG
                     GGDAKAKDAPKADTSDEAEGKAGQDEAEPAEQEEIHNDGRIRVGSKVIAGTVLARVAD
                     GKDGVDPHINFSIRPAGKGAPRIDPKPILDGWKLLEATAIYRAKGKNPFTSRLSGAGV
                     LLLSKEALIKRVLADDGLSIYECGRDDIATGKIDRRILAMLEYLRAKGFELTITSLNC
                     GHGFLTTSGNVSEHSTGDAVDIAAINGTPVLGHQGPGSVTSQLIREVLTLQGTMEPHQ
                     VISLEDWPGPVSFPLSDHADHVHVGYHAIGDNPLESRFSPLLKPDQWERLINRLGEIE
                     NPEVAIRPSSSSLPADEPDGGTPAD"
     gene            3615..4493
                     /locus_tag="VFB52_08420"
     CDS             3615..4493
                     /locus_tag="VFB52_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06397.1"
                     /translation="MSKLFGFAQFEFAGRLALADGRYLARDPLAGGAESVLVLNTLGA
                     PRPTSRRRRRPRAAEVEAEQGALPLTRATAIRAFAPFDSVEAAELWLDQASEAEDTTD
                     VLVSEGIALLNRALHAQAVAAADPQAQELTPERAVAVRIGYGTGEETADGLYSAAREV
                     DVWASGASRRRRREEDLRPQERVAAILGGRERLDVCETLLLRARADLDAERGREAALQ
                     LRVGVEALLAELADAVDDLGHQKDVGALRERRTEVGEAANIALSADLDPAHAAAVREL
                     LELSERILRRRRVLRG"
     gene            complement(4515..5165)
                     /locus_tag="VFB52_08425"
     CDS             complement(4515..5165)
                     /locus_tag="VFB52_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZO06398.1"
                     /translation="MNLPEAVVFDNDGLLLDTESVWTRAEEDLFERRGTAFTPADKLK
                     LVGTSAEIAGGILEERLGEPGRAAELIEELNELVVAELEHGVEAMIGARDLLHALKER
                     GTPIGLVSNSPLIFVQRSLEIVGFVDHFDVVLSAHEVAAPKPAPDPYLEACRRLGVEA
                     GPNVVALEDSPTGVAAAVAAGLTVIGVPSVAGVALEEAHHIAESLQDAIVADRLRL"
     gene            complement(5162..5929)
                     /locus_tag="VFB52_08430"
     CDS             complement(5162..5929)
                     /locus_tag="VFB52_08430"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZO06399.1"
                     /translation="MERQVAVSTQVERLAELLRQSRCAVALTGAGISVPSGIPDFRSP
                     GTGLWENVDPMEVAHIDVFERDPARFWTYYRPRFQTLGDKRPNRAHEALAELERRGLI
                     GGVITQNIDRLHRAGGSQNVVEVHGSIETSSCRSCARSFGLEEVEGIFDAAGVAICTE
                     CGGAVKPDVVLFGELLPEAAMAAAQELAEGADLMICVGSSLAVYPVAGLPQLTLQRGG
                     KLVIVTKGPTEYDADAELKLEGEVDEELSALLEVLGE"
     gene            complement(5914..>7567)
                     /locus_tag="VFB52_08435"
     CDS             complement(5914..>7567)
                     /locus_tag="VFB52_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO06400.1"
                     /translation="PGHAILASNTSALSITEIGESTLRADKVVGFHFFYPASVMPLVE
                     VIVGEDTSQETATAAYNFAQAIKKQPIVCGEVPGFVVNKILMATIGEIWRAQEEHGLS
                     LAAIDQAIAEAKLAPMGPFFLTDLLGLDTVLHVAEHLNESYGEHFYVHKGLQGLVADG
                     KLGAKSGGEGFFKDGQQTIPGEAEPDAEELIAMFTSRALIEACLLVEEGICSVRDVDL
                     GMMTGAGLDPRRGLLPPFWKADVEGLGKALETIERLEEKYGERFTPPVTLKRLVAQGR
                     LGLASGQGFYPYPQPDEGEQPDTVKLETRGDVAIAWLANLPMNAISPDVIRDLQKVWD
                     RVKADGDVRAMVVASSVPIVFSAGADIKAFTQMDEAAGKALIEEGHALLREFGTESVA
                     TVAAVNSLAFGGGCELAMACDFRIAAEAAVFGQPEVKLGIIPGFGGTQRLPRLVGESK
                     ALEMNLIGDAVLSEEALELGLVNRVVPDHELFETALMWARKLAGQAPIAVGQIKQVSH
                     KGDLDEGIEAEKQGFATAFFSADAKEGISAFLGKRTPKWSGK"
BASE COUNT         1186 a         2741 c         2551 g         1089 t
ORIGIN      
        1 gctcgtgctc gccgacctcg ccgagctcgg cctgcgccgg gcggtccggg tcgggaccgc
       61 gaccgggctc gaccccgagc ttgaagcggg cgagttggtc gtggtcagcg aggcgcacgc
      121 ctggggcgga cccggcggcg gtgaaggcgc ggcggttttg cccgacgccg agctggccga
      181 ggcgctcgcg cgccaactcg ggatcgcggc gcgaccgggc tcggtcgcca gcctcgacac
      241 cctgcacggc cagctcgatg aggttgtcgc cgcgagcctc gggtccgacg tcgccgacat
      301 gcaaaccgcg gcgctgctca cccgcggtcg cgagctcgag ctggcggtcg cggcggtcct
      361 cgtcgtcagc gagaccggtg ccggcgagaa actcgacgac gagccgttgg agttggccgc
      421 gaaacgggcc ggaaccgccg cggcggccgt actctaaccc tcaagtcgaa ggttaggctt
      481 tcgccttttt cggcgctttc ttctctttct tctcgctctg ctcgtcgtcc tgcttggcca
      541 gctggcgccg cagcgcccca acctcgcggc tgaggtcgtc gatctgctcc tcgacctcct
      601 cgagccgctg cgagaacgag cgcaggcgcc gctcgacccg ttccaggtcg ctcgccgatg
      661 ggatgtcgag cgcgctcatc gccgcccgct gcgcctcgat cgccttctcg cgggcaccga
      721 gcgccgcggt gaccgcgttg tgcagcgtcg ggttgtcgat caaagcctgc gccagctccc
      781 ccagggcctg ctcggagcgc gcacgcaggc tgtcctggct gtattgttga ccatcctgat
      841 catgaaactc gtccattacg cctccggtcc ccttttgcat ctcattcacg tcagcttaac
      901 tagcgcatcc ggcacggctg aaacgctgcc gttgcaggta tgacgccgtt ggagattaag
      961 ggcaggttgg ggggcggcga gagccgttgc acccccctgg gcgagtgggt accttggtca
     1021 cgcctggcgc tctgggaggg gacggtcgcg gtagcggcgt tccgcaacgg cgagcgcggg
     1081 taacgaggcg cactgggatg agaaccaaga tcgcactgat cgtcgcgctt gccagcttcc
     1141 tggcggcgac ggcattgagc gcagcagtcg cacctgctga ttcgggcacc ggctcggtca
     1201 tcggtggcac cccgctggcg ccgatcgcgc ctgccgcgag cccgaccgcg ccggcctgct
     1261 cgaaccagat cgacgacgac ggcgacggcc tcgtcgacct cgacgacagc gcttgcaccg
     1321 aacccgcgtg gaccagcgag gaagcggctc ccgcggaaac gaccgctcct gttccaaccc
     1381 ctgcggctcc gcccgcagaa ggcgatgaca aggacaaggg cgagaaggat tcctcgaagg
     1441 acggcgtcaa gcagggtgac tcgatcggcg gcgacgcggc caagcacaag cgcggcggcg
     1501 ccaccgtcaa cgagccgctc ggcgacacca gcggcggccg caagtccgac ggcgcgatcg
     1561 aggcgccgag cgcaaccggc aacggcgacc agccgctcga cagcgacggc aacgggggcg
     1621 cccagttcaa cgacggcggc agccccaccc tcggcaaccc gacgacgacg atcgcgccct
     1681 tcggcccggc tccgatcggc gtccccaact tcatcatcga ctcgttcgaa atcccgccct
     1741 tcctgctgcc gatctaccag gcctgcggca ccgaatacgg ggtcccctgg gaggtgctcg
     1801 cctcgatcaa caaaatcgag accgggttcg gcaccaacct caacgtctcc accgccggcg
     1861 cgatcgggtg gatgcagttc ctgccttcga gctgggaggc cttcggggtc gacgccaacg
     1921 gcgaccgcaa gaaagatccc tacaacccgg ttgacgcgat ctgcgccgcg gccaactacc
     1981 tggagatcgc cggcgccaaa gagaacctct acgacgcgat cttcgcctac aaccacgccg
     2041 actggtacgt gcaggaggtg ctgctctacg cgcgcgccta cggccgcctg ccctccgacc
     2101 tggtcggctc gctgaccggc ctcaccgagg gcgcccactt cccgatcgcc gccgatgcca
     2161 gctacgccga cgacgtcgcc gcgcgggcgt cactgaaaag cggggactac acggcggcgg
     2221 gcagcgccgc cgaagtgatc tccaactccc ccaccgagcg cgggatcaac atcttcgccg
     2281 acgagggtgc gccggttgtc gccgtcaacg acggggtggt tcgcaagacg ggccactcgg
     2341 agaagctcgg caacttcgtc gtgctcgaag acacctacgg caaccgctac acctacgccg
     2401 ccctcggcga gctggtccgc gacgaacggc tggtggtgat gccgagcggt gaggagaaac
     2461 gactgccggt cgaaagcgag aacctgcgac cgcgactgcg ggcgctgccc ggccggatcg
     2521 aggacgacgg cctcggccat gccgagggca aaccgtcggg cggcaacagc gccggcgaac
     2581 tgcagcccga cgacgtgatc ggcagcggcg ccagcgaagg tggcggcgac gccaaggcca
     2641 aggacgcgcc caaagccgac accagcgatg aggccgaggg caaagccggg caggacgagg
     2701 cagagccggc cgagcaggaa gaaatccaca acgacggccg catccgggtc ggctcgaaag
     2761 tgatcgccgg gaccgtgttg gcgcgggtcg ccgacggcaa ggacggcgtc gacccgcaca
     2821 tcaacttctc gatccgcccg gccggcaaag gcgcgccgcg gatcgacccg aagccgatcc
     2881 tcgacggctg gaagctgctc gaggcgaccg cgatctaccg cgccaaaggc aagaacccct
     2941 tcacctcccg tctcagcggc gccggcgtgc tgctgctctc caaggaggcg ctgatcaaac
     3001 gggtgctcgc cgacgacggc ctctcgatct acgaatgcgg ccgcgacgac atcgccaccg
     3061 gcaagatcga ccgccgcatc ctggcgatgc tcgagtacct gcgcgccaag ggcttcgagc
     3121 tgacgatcac ctcgctgaac tgcggccacg gcttcctcac cacctcgggc aacgtctccg
     3181 agcactcgac cggcgatgcg gtcgacatcg ccgcaatcaa cggcaccccg gtgctcggcc
     3241 accagggccc gggctcggtg accagccagc tgatccgcga ggtcctgacc ctgcagggca
     3301 cgatggaacc gcaccaggtg atctcgctcg aagactggcc cggcccggtc agcttcccgc
     3361 tttccgacca cgccgaccac gtccacgtcg gctaccacgc gatcggcgac aacccgctcg
     3421 aatcgcgctt ctcgccgctg ctcaaaccgg accagtggga gcgcctgatc aaccgcctcg
     3481 gcgagatcga gaaccccgaa gtggcgatcc ggccctcgtc ctcctcgctg ccggccgacg
     3541 agcccgacgg cggcaccccc gctgactgac agcgccgcta accggccgct gcgctgaccg
     3601 ggagaatccc ggtcgtgtcc aaactattcg ggttcgcgca gttcgagttc gccggccgcc
     3661 tggcgctcgc cgacggccgc tacctggccc gcgacccgct cgccggcggg gccgagagcg
     3721 tgctggtcct gaacaccctc ggcgccccgc ggccgacctc gcgccgtcgc cggcgcccgc
     3781 gggcagccga ggtcgaggcc gagcagggtg cgctgccgct gacccgggcg accgcgatcc
     3841 gcgccttcgc gcctttcgac tcggtcgagg cggcagagct gtggctcgac caggcgagcg
     3901 aggcggagga caccacggac gtgctggtca gcgaggggat cgcgctgctc aaccgcgccc
     3961 tgcacgcgca ggcggtcgcg gccgccgacc cgcaggcgca ggagctgacg cccgagcggg
     4021 cggtggcggt gcgaatcggc tacgggaccg gcgaagagac cgccgacggc ctctacagcg
     4081 ccgcccgtga ggtcgacgtc tgggccagcg gcgcttcacg gcgccggcgc cgtgaagaag
     4141 acctgcgccc ccaggaacgg gtcgcggcga tccttggcgg ccgcgagcgg cttgacgtct
     4201 gcgagaccct gctgctgcgc gcccgcgccg acctcgacgc cgagcgcggc cgcgaggcgg
     4261 ccctgcagct gcgggtcggg gtcgaggcgc tgctggccga gctcgccgat gcggtcgacg
     4321 acctcggcca ccagaaagac gtcggcgccc tgcgcgagcg ccgcaccgag gtcggtgagg
     4381 cggcgaacat cgcgctgagc gcggacctcg accccgctca cgccgccgcg gtccgcgaac
     4441 tactcgagct cagcgagcgc atcctgcgcc gcaggcgcgt gcttcgcggc tagacctaag
     4501 cctaaagctg agggttagag tcgaagccga tcggcgacga tcgcgtcctg cagcgactcg
     4561 gcgatgtggt gcgcttcctc cagcgcgacg ccagcgaccg aggggacgcc gatcacagtc
     4621 aaaccggcgg caaccgcggc cgcgaccccg gtcggcgagt cctccagcgc gaccacgttc
     4681 gggcccgcct cgacgcccag gcggcgacag gcctccaggt aggggtcggg ggccggcttc
     4741 ggcgccgcaa cctcgtgggc gctgagcacg acgtcgaagt ggtcgacgaa gccgacgatc
     4801 tcgagcgagc gctggacgaa gatcagcggc gagttggaga ccaggccgat cggcgtcccc
     4861 cgctctttca gcgcatggag tagatctcgc gccccgatca tcgcctccac cccgtgctcg
     4921 agctcggcga cgaccagctc gttcagctcc tcgatcagct ccgccgcgcg gccgggctca
     4981 cccagccgtt cctcgaggat gccgccggcg atctcagccg aagtgccgac cagcttcagc
     5041 ttgtcggctg gtgtgaacgc ggtgccgcgg cgctcgaaca ggtcttcctc ggccctggtc
     5101 cagaccgact cggtgtcgag cagcagcccg tcgttgtcga agacgaccgc ctccggaagg
     5161 ttcattcgcc cagcacctcc agcagcgccg acagctcctc gtcgacctcg ccctcaagct
     5221 tcagctcggc gtcggcgtcg tactcggtcg ggcccttggt cacgatcacc agcttgccac
     5281 ctcgctgcag ggtcagctgt ggcaatccgg caaccgggta gacggccagc gaggagccga
     5341 cgcagatcat caggtcggcg ccttcagcca gctcctgcgc cgccgccatc gcggcttccg
     5401 ggagaagctc gccgaagagg acgacgtcgg gtttgaccgc accaccgcac tccgtgcaga
     5461 tcgccacccc cgcggcgtcg aagatccctt cgacctcctc gaggccgaag ctccgcgcgc
     5521 agctgcggca gctcgaggtc tcgatcgagc cgtgcacctc gaccacgttc tgcgagccgc
     5581 cggcccggtg caggcgatcg atgttctggg tgatcactcc cccgatcagc ccgcgccgct
     5641 cgagctcggc gagcgcctcg tgggcgcggt tcggccgttt gtccccgagc gtctgaaagc
     5701 gcggccggta gtaggtccag aagcgggccg ggtcgcgttc gaagacgtcg atgtgggcga
     5761 cctccatcgg gtcgacgttc tcccagagcc cggtaccggg gctgcggaag tcggggatcc
     5821 cggacggcac cgagatcccc gccccggtca gggcgacggc gcagcgcgac tggcggagca
     5881 actccgccag tcgctcgacc tgtgtcgaga ccgctacttg ccgctccact ttggcgtccg
     5941 cttgccgagg aaggcgctga tgccctcttt cgcgtccgcg gagaagaagg cggtcgcgaa
     6001 accctgcttc tcggcctcga tgccctcgtc gaggtcgccc ttgtgggaga cctgcttgat
     6061 ctggccgacc gcgatcggtg cctgcccggc gagcttgcgg gcccacatca gcgccgtctc
     6121 gaacagctcg tggtcgggga cgacgcggtt gacgaggccg agttcgagcg cctcctcgga
     6181 gagcaccgcg tcaccgatca ggttcatctc cagcgccttg ctctcgccga ccaggcgcgg
     6241 cagccgctgg gtgccgccga acccggggat gatccccagc ttcacctcgg gctggccgaa
     6301 gacggcggcc tcggcggcga tccggaagtc gcaggccatc gccagctcgc aaccgccgcc
     6361 gaaggcgagc gagttgaccg cggcgaccgt ggccaccgac tcggtgccga actcgcgcaa
     6421 cagcgcatgg ccctcctcga tcagcgcttt gcccgccgcc tcgtccatct gcgtgaacgc
     6481 cttgatgtcg gcgccggccg agaacacgat cggcaccgag gaggcgacga ccatcgcccg
     6541 cacgtccccg tccgccttga cccggtccca gaccttctgc aggtcgcgga tcacgtcggg
     6601 ggagattgcg ttcatcggca ggttggcgag ccaggcgatc gcgacgtcgc cgcgggtctc
     6661 cagcttcacc gtgtcgggct gctcgccctc gtcgggctgc gggtaggggt agaagccctg
     6721 gccgctggcc aggccgagcc ggccctgggc gaccagccgc ttcagggtca ccggcggggt
     6781 gaagcgctcg ccgtatttct cctccaggcg ctcgatcgtc tccagcgcct tgccgagccc
     6841 ttcgacgtcg gctttccaga acgggggcag cagcccccgg cgcgggtcga ggccggcccc
     6901 ggtcatcatc ccgaggtcga cgtcgcggac cgagcagatc ccctcctcga ccagcaggca
     6961 ggcctcgatc agcgcgcggc tggtgaacat cgcgatcagc tcctcggcgt cgggctcagc
     7021 ctcgccgggg atcgtctgct gaccgtcctt gaagaagccc tcgccgccgc tcttggcgcc
     7081 gagcttgccg tccgcgacca gcccctgcag gcccttgtgg acgtagaagt gctcgccgta
     7141 ggactcgttc aggtgctcgg cgacgtgcag cacggtgtcg aggccgagca ggtcggtgag
     7201 gaagaacggc cccatcgggg ccagtttcgc ctcggcgatc gcctggtcga tcgcggcgag
     7261 cgagagcccg tgctcctcct gcgcccgcca gatctcgcca atcgtcgcca tcaggatttt
     7321 gttgacgacg aagccgggga cctcgccgca gacgatcggc tgcttcttga tcgcctgggc
     7381 gaagttgtag gcggcggtcg cggtctcctg cgaggtgtcc tcgccgacga ttacctcgac
     7441 cagcggcatc accgacgccg ggtagaagaa gtgaaagccg acgaccttgt ccgcgcgcag
     7501 ggtcgattcg ccgatctcgg tgatcgagag cgccgaggtg ttcgaggcga ggatcgcgtg
     7561 gccgggc
//
LOCUS       DASRIG010000311         7390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2771364, whole genome shotgun sequence.
ACCESSION   DASRIG010000311 DASRIG010000000
VERSION     DASRIG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771364"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFB52_08440"
     CDS             complement(<1..1268)
                     /locus_tag="VFB52_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HZO06401.1"
                     /translation="MGLVAAGGEESCQTMTYQQPDALWLLGLIPVVVILHLLRAAANR
                     QLVPSAFLWRELGRDPEVARRWRPPRPTLVLFLQILAIAAAAFALSMPRLTTPPGRHV
                     VVVLDASGSMLATDERPTRFDEAVRRVRLLLATLSPTDVATIVRAGPRPRPVATAVDS
                     IAAQGALGEVRPGGGTTAMREALFVASDLARRQQDRAAEIVVYSDGAFTDPGDLSTLG
                     VTTRFEIVGRETANRAVTGLSVARQPGGPGTQSAVARLANYGDQPARVGLSLFVDDVL
                     SDRRDVDLAAKGQTVAAFEVPAGVRRVAVKLAGGDALPADDFAEVSVDIGFVRRVLLV
                     TRRPEALERALKAIPDLRVDTVSPERYDGSGAEIVVLDGVVPPTLPSGQLIVVNPPPD
                     QTLLPIRSEIQGVQFSDFDPRHPLLQAIDLS"
     gene            complement(1373..5152)
                     /locus_tag="VFB52_08445"
     CDS             complement(1373..5152)
                     /locus_tag="VFB52_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO06402.1"
                     /translation="MHLKRLDLVGFKSFANRTTFLFEPGISVMCGPNGSGKSNVADAV
                     RWALGEQSARAIRGRRGEDVIFVGSQGRQPLGLAEVSLTLDNSEGRIPLDYQEVRVTR
                     RLYRSGEAEYLVNGSKVRLKDIHEWLLHAALDAESYVVVGQGSVDQLILQRPEERRVV
                     IDNAADIRRHQTRLHETRNRLASTEENLLRCRAVIAELEPHVTRLRAQAERAERAQVL
                     RAELGTLAGRWLRHALAGARAELARARAEAAAARAQAERQEAELGDLEALARAADRAA
                     TAAEATVAEIEPRLAAARDEVARIGRDLARAEERLAGADESEARLLAEVERQREREAA
                     LTAEHAALQQQLAAARAAVTDAEHAAQAAAEAGRAEEEEARRVEGELAAARRRLAALD
                     GELRSAASAAEEARGRIERLERRQVRLAEDLERLIRDLERARTEAAARAAAALSAASA
                     LTDARRAREALLERREEGRQRVDRLRAEQHRLSGDRQRLEIARQAAEESGGSQKGSGR
                     DVLLKSDAVAARGLLGGELTVPERYRAAIGAALGERAQIVVLAECSGPDAAVHLIRQR
                     DLDRTGLVESPTPQPAARREYRLRRHYPPCAGEGGKISPSFAHAVGVGGPMGGVGVGD
                     TLRSLLDGLEILGFADELVEAPPELESLVQRVLGSTVVVATLDLAREAASRLASAPAG
                     WQVATLDGVLLRWTGEWWAGRDRQAERLVRRRAELAEIDGRMASVAARLQEIEQQLAE
                     ADAGLRQLAADERSVRDALAAAESADRKAALEAQAAAAQASRLERELRDAQAAGPTLE
                     TDLQRARAQRERADTHLEETDRRRFAGLEALREAEGRQVGLSERLAEGRAARANLQAD
                     LARRRAEIQGVEALLSRLGADLESALRAVTESERRAESERGRRGDLEGVSEGLRGTLQ
                     RAESAVPPLVESLAAAREARQQARAKREAIEQRASELRAVARSGRTVLEATAIAESRA
                     ADRLERVRREAQEYVDDLAGADGEVQLRLDLGDEPTAEDQTVDDEPGPPFDPEAARRR
                     MGVLRRELRAIGDVGEATLAEFRELSERHRFLVEQVADLELAGGELLRVMEELTGLMR
                     EAFDTAFANVNAAFGEYFARLFAGGHAELVLSRPEEVLESGVDIVARPPGKRLQPLVS
                     LSGGERALTMVALIFALLKTNPAPFCVLDEVDAALDESNVRRFTSVLAELSDRTQFVV
                     VSHNRATMESAQALYGISMDTMGVSTVVSLKLPNESVTNGSKNGAAVARNGMIST"
     gene            complement(5152..5973)
                     /gene="rnc"
                     /locus_tag="VFB52_08450"
     CDS             complement(5152..5973)
                     /gene="rnc"
                     /locus_tag="VFB52_08450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZO06403.1"
                     /translation="MSDLEAVRPPEPDLDELERRLGVTFKDRTLLRQAVIHKSLTNDL
                     GESGLGSNERLEFLGDSVLGMLVSEQLYRAYPDRDEGQLTLLRSALVRASSLAGWARQ
                     LDLGAFVMVGRGEIRGDGRNRDALLSSAFEAVLGAVFVDRGFHAAKQLLMRFLRPDLA
                     QVGQRQVIDAKSRLQQVSQARFSVTPTYEVLDVSGAGHSPIFTIRVTAGGGVTAVAEG
                     RSKQTAQQTAAAEALATIEALPVELPPPTEALADSTDGAGDPAEAVADPVEAPSS"
     gene            complement(5966..7312)
                     /locus_tag="VFB52_08455"
     CDS             complement(5966..7312)
                     /locus_tag="VFB52_08455"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZO06404.1"
                     /translation="MDELLGRARFESLADRALDASRADQTEVVVIGVDSALTRFANSG
                     IHQNVAERNVQVRVRAIVGRRAGVATTNDLSDAALARVVERAVEAAKRQPEDPGLPDL
                     PGPLPAEPVPSFSQATADCTPEQRARMVRPICALAVEHGLKASGACSTETTEIGIANS
                     RGVRRHELRSASSLLTVILDDEGSGYAERTARDIDSIDAEALGREAVDKAVRSRGAER
                     FEPGEYPVVLEAYAVGEMLMYLAYMGFGALALQEGTSFLRGRLGQRIVDPRISVSDDG
                     RNPLGLPASFDYEGVPKQRVALIEDGVAAGVVYDTKSAAREGRPSTGHGLPAPSTFGP
                     FPSHLSMAAGDTPPAELAKGIERGLWVTRFNYVNVVKSDRAVLTGLTKDGTFLIERGE
                     VTRPVKNLRFTQGVLEAWSGLRALGAERRLIEGWGGAVLAPAMRLDAFRFTGVSDV"
BASE COUNT          967 a         2643 c         2792 g          988 t
ORIGIN      
        1 gacaggtcga tcgcctggag cagcgggtgg cgcgggtcga agtcgctgaa ctggacgccc
       61 tggatctcgg accggatcgg caggagcgtc tggtcgggcg gcgggttgac gacgatcaac
      121 tggccgctcg ggagggtcgg cgggacgacc ccgtccagca ccacgatctc ggcgccagag
      181 ccgtcgtacc gctcgggcga gacagtgtcc accctgaggt cggggatcgc cttgagggcg
      241 cgctccaacg cctcgggacg gcgggtcacc agcaggacgc gccgcacgaa cccgatgtcg
      301 acgctgacct cggcgaagtc gtcggccggg agggcgtcgc cgccggccag cttgacggcc
      361 acgcgccgga caccggccgg cacctcgaaa gcggcgacgg tctggccctt cgccgcgagg
      421 tcgacgtcgc ggcggtcgct caggacgtcg tcgacgaaca ggctcaggcc gaccctggcc
      481 ggctggtccc cgtagttggc cagccgcgcg acggcgctct gggtaccggg accgcccggc
      541 tgccgtgcga cgctgaggcc ggtcaccgct cggttcgcgg tctcgcggcc gacgatctcg
      601 aagcgggtgg tgacgccgag cgtcgagaga tcgcccggat cggtgaaggc gccgtccgag
      661 tagaccacga tctcggcggc gcggtcctgc tggcggcggg ccaggtcgga cgccacgaac
      721 agcgcctcgc gcatggcggt cgtgccgccg ccgggccgca cctcgccgag ggcaccttgc
      781 gcggcgatcg agtcgacggc cgtggcgacg gggcgcggcc gagggccggc ccggacgatc
      841 gtcgcgacgt cggtcggcga gagggtcgcc aggagcaggc ggacacggcg caccgcctcg
      901 tcgaagcggg tcgggcgctc gtcggtcgcc agcatgctgc ccgaggcgtc cagaacgacc
      961 acgacatgcc ggcccggcgg ggtcgtcagg cgcggcatcg agagcgcgaa cgcggccgcg
     1021 gcgatcgcca ggatctggag gaacagcacc agcgtcgggc ggggcgggcg ccagcgccgg
     1081 gcgacctcgg gatcgcggcc aagctcgcgc cagaggaagg cgctgggtac gagctgccgg
     1141 ttcgccgccg cgcgcagcag gtgcaggatg acgacgaccg ggatcaagcc gaggagccag
     1201 agcgcgtctg gctgctggta ggtcatcgtc tggcaggact cctcgccacc agcagcgacc
     1261 agtcccacga gccgggagcg attggagcgg agccggcgat ggtcggcaag gcctgagggt
     1321 ccttcggaac gtgtctcaga ctgtaccaga cagtccggcc cggacggtcg ctttacgttg
     1381 agatcatccc atttctcgcg acggccgcgc cgttcttcga gccgttcgtg acgctttcgt
     1441 tcggcagctt caaggagacg accgtgctca cgcccatcgt gtccatcgag atgccgtaga
     1501 gcgcctgcgc cgactccatc gtggcccggt tgtgcgagac gacaacgaac tgggtccggt
     1561 cgctcagctc cgcgagcacc gaggtgaagc ggcgcacgtt cgactcgtcc agcgcggcgt
     1621 ctacttcgtc aaggacgcag aacggggccg ggttggtctt cagcagcgcg aagatcaggg
     1681 cgaccatcgt cagcgctcgc tcgccgcccg agaggctcac cagcggctgg aggcgcttac
     1741 cgggcggacg ggccacgatg tcgacgccac tctccagcac ctcctcgggg cggctcagga
     1801 ccagctcggc gtggccgccg gcgaacaggc gcgcgaagta ctcgccgaag gcggcgttga
     1861 cgttggcgaa cgccgtgtcg aacgcctcgc gcatcagtcc ggtcagctct tccatgacgc
     1921 gcagcagctc gccaccggcc agttcgagat cggcgacctg ctcgaccagg aagcgatggc
     1981 gctcactcag ctcgcggaac tcggccaggg tcgcctcgcc gacgtcgccg atggcgcgca
     2041 gctcgcgccg gaggacgccc atccgacgcc gtgcggcctc cggatcgaac ggcgggccgg
     2101 gctcgtcgtc gaccgtctga tcctcggcgg tcggctcatc gccaaggtcg agacgcagct
     2161 ggacttcgcc gtcggcgccc gccaggtcgt cgacgtactc ctgggcctcg cgccggacac
     2221 gctccagccg gtcggctgcg cgactctcgg caatggccgt cgcctccagc acggtgcggc
     2281 cggaccgcgc gacggcccgc agctcggacg cccgctgctc gatcgcctcg cgcttcgcgc
     2341 gggcctgctg gcgggcctcg cgggcggcgg cgagagattc gaccagcggc gggacggcgg
     2401 actcggcccg ctggagggtg ccgcgcagcc cctcggaaac gccttcgaga tcgccgcgtc
     2461 ggccgcgctc ggactcggcg cggcgctcgc tctcggttac agcacggagc gcagattcga
     2521 ggtcggcgcc gagccgggag agcagcgcct cgacaccctg gatctcggcg cggcggcgcg
     2581 ccaggtcggc ctgaaggttc gcgcgggccg cccggccctc ggcgaggcgc tccgacaggc
     2641 cgacctgccg gccttcggcc tcgcgcagcg cctccaggcc agcgaatcgg cggcggtcgg
     2701 tctcctccag gtgcgtgtcg gcgcgttcgc gctgggcgcg ggcccgctgg aggtctgtct
     2761 ccagggtcgg gccggccgcc tgggcatccc tgagctcccg ttccagccgc gaggcctggg
     2821 cggcagccgc ctgggcctcc agggcggcct tacggtcggc cgactcggcg gcggcgaggg
     2881 cgtcgcggac ggagcgctcg tcggcggcga gctgtctgag accggcatcg gcctcggcga
     2941 gctgctgctc gatctcctgc agcctcgccg cgaccgacgc catccttccg tcgatctcgg
     3001 cgagctcggc gcgtcggcgg accagccgct cggcctggcg gtcgcggccg gcccaccact
     3061 cgccggtcca gcggaggagg acgccgtcga gggtggcgac ctgccagccg gcgggggccg
     3121 aggcgagacg actcgcggcc tcgcgggcga ggtcgagtgt ggcgacgacg acggtggagc
     3181 cgagaacgcg ctggacaagc gactccagct ccgggggcgc ctcgacgagc tcgtcggcga
     3241 acccgaggat ttcgaggccg tcgagcagcg aacgaagggt atcccccacc ccaacccctc
     3301 ccatggggcc ccctaccccc acggcgtgcg cgaaggaggg gcttatcttc cccccctccc
     3361 ccgcgcacgg gggataatgt cggcgaagcc gatattcgcg ccgcgcagcg ggctgggggg
     3421 taggggactc cacgagccca gttcggtcga ggtcgcgctg gcggatcagg tggacggcgg
     3481 cgtcgggacc ggagcactcc gccaggacga cgatctgcgc acgctcgccg agcgctgccc
     3541 cgatggccgc ccggtagcgc tcgggaacgg tcagctcacc accgagcagg ccgcgcgcgg
     3601 cgacggcatc cgacttgagg agcacgtcgc ggcccgagcc cttctggctg ccgccgctct
     3661 cctcggcggc ctgccgcgcg atctccaggc gctggcggtc ccccgacagc cggtgctgct
     3721 cggcgcgcag ccggtcgacg cgctggcggc cctcctcgcg gcgctccagc aacgcctcgc
     3781 gggcgcggcg ggcgtcggtc agggcggagg cggcgctgag cgcggcggca gcgcgagcgg
     3841 cggcctcggt gcgcgcccgt tcaaggtcgc ggatcagcct ttcgagatcc tcggcgaggc
     3901 ggacctggcg gcgctccagc cgctcgatcc gaccgcgggc ctcttcggcg gccgacgcgg
     3961 cgctccgaag ctcgccgtcc agggcggcaa gccgacggcg ggcggccgct agctcgccct
     4021 cgacgcggcg cgcctcctcc tcctcggcgc ggccggcctc ggcggcggcc tgggcggcat
     4081 gctcggcgtc ggtcacggcg gcgcgggccg ctgcgagctg ctgctggagc gccgcgtgct
     4141 cggcggtcag cgccgcttcg cgctcgcgct ggcgctcgac ctcagccagc aggcgcgctt
     4201 cggactcgtc ggcgccggcc agccgctcct cggctcgggc caggtcgcgt ccgatcctgg
     4261 cgacctcgtc gcgggcggcg gccaatcgcg gctcgatctc ggcgacggtc gcctcggcgg
     4321 cggtcgcggc ccggtcggca gcgcgggcga gcgcttcgag gtcgccgagc tcggcctcct
     4381 ggcgctcggc ctgggcgcgg gcggcggcgg cctccgcgcg ggcgcgggcg agttcggcgc
     4441 gggcgcctgc gagtgcgtgg cgcagccagc ggccggccag ggtgccaagc tcggcgcgga
     4501 ggacctgggc acgctcggcg cgctcggcct gggcgcgcag gcgggtgacg tgcggctcca
     4561 attcggcgat tacggcccgg cagcgcagca ggttctcctc ggtggaagcc agccggttgc
     4621 gggtctcgtg aaggcgggtc tggtggcggc ggatgtcggc ggcgttgtcg atgacgaccc
     4681 gacgttcctc cggccgctga aggatcagct ggtcgaccga gccttgcccg accaccacgt
     4741 acgactcggc gtcgagggcg gcgtgcagca gccattcgtg gatgtccttc agccggacct
     4801 tcgagccgtt gaccaggtac tcggcctcgc cggagcggta gagccggcgg gtgaccctga
     4861 cctcctggta atcgagcggg atccgtccct cggagttgtc gagggtcagc gagacctcgg
     4921 ccaggcccag cggctgccgc ccctgcgagc cgacgaagat gacgtcctcg ccccgccggc
     4981 cccggatcgc ccgcgcgctt tgctcgccga gcgcccagcg gacggcgtcg gcgacgttgc
     5041 tcttgccgga gccgttcggg ccgcacatca ccgagatgcc gggctcgaac aggaaggtcg
     5101 tgcggttagc gaagctcttg aatccgacga ggtcgaggcg cttgagatgc atcaggatga
     5161 cggcgcctcg acaggatcgg cgacggcctc ggccgggtcg ccagcgccat cagtcgagtc
     5221 ggccagggcc tcggtgggcg gcggcagctc gaccggaagc gcctcgatcg tcgccagcgc
     5281 ctcggcggcg gccgtctgct gggccgtctg cttgctcctc ccctcggcga cggccgtgac
     5341 gccgccgccg gccgtgaccc tgatggtgaa gatcgggctg tggccggcgc ccgagacgtc
     5401 gagcacctcg taggtcggcg tcaccgagaa gcgggcctgg ctgacctgct ggagccggga
     5461 cttggcgtcg atcacctggc gctggccgac ctgcgcgagg tccggccgga ggaaccgcat
     5521 caggagctgc ttcgccgcgt ggaagccccg gtccacgaag acggcgccca ggaccgcctc
     5581 gaaggcgctg ctcagcaggg cgtcgcggtt gcggccgtcg cctcgaatct cgccgcgccc
     5641 gaccatcacg aacgcgccga ggtcgagctg gcgcgcccag ccggccaggg acgaggcgcg
     5701 gaccagcgcg gaccgcagca gcgttaactg gccctcgtcg cgatccgggt aggcgcggta
     5761 gagctgctcg gagaccagca tcccgagaac cgagtcgccg aggaactcca gccgctcgtt
     5821 cgagccgagg ccgctctcgc cgagatcgtt ggtgagcgac ttgtggatca cggcctggcg
     5881 cagcagcgtc cggtctttga acgtcacgcc cagccggcgc tccagctcgt ccaggtcggg
     5941 ctcgggcggg cggacggcct ccaggtcaga catcgctgac cccggtgaag cggaaggcgt
     6001 cgagtcgcat ggccggagcg agcaccgcgc cgccccaacc ttcgatcaga cggcgctcgg
     6061 cgccgagggc ccggaggccg ctccacgctt cgaggacgcc ctgggtgaag cggaggttct
     6121 tgaccgggcg ggtgacctcg ccgcgctcga tcaggaacgt gccgtccttg gtgagcccgg
     6181 tcagcaccgc ccggtccgac ttgacgacgt tgacgtagtt gaaccgagtg acccagaggc
     6241 cgcgctcgat ccccttcgcc agctcggcgg gcggcgtatc gccggcggcc atcgacaggt
     6301 gcgaggggaa cggcccgaac gtgctcgggg cggggaggcc gtggccggtc gacgggcggc
     6361 cctcgcgggc ggccgacttc gtgtcgtaga cgacgccggc cgcgacgccg tcctcgatca
     6421 gggcgacgcg ctgcttcggg acgccctcgt agtcgaagct ggccggcagg cccagggggt
     6481 tgcgcccatc gtccgagacc gagatgcgcg ggtcgacgat ccgctggccg agccggccgc
     6541 gcaggaagct ggtgccctcc tggagggcga gcgccccgaa ccccatgtag gcgaggtaca
     6601 tcagcatctc gccgacggcg tacgcttcca ggacgaccgg gtattcgccg ggctcgaagc
     6661 gctcggcgcc gcggctgcgg accgccttgt cgaccgcctc gcgaccgagc gcctcggcgt
     6721 cgatggagtc aatgtcgcgg gcggtccgct cggcgtagcc cgagccctcg tcgtcaagga
     6781 tcacggtcag gaggctggag gccgatcgca gctcgtggcg acggacgccg cgcgagttgg
     6841 cgatgccgat ctcggtcgtc tcggtcgagc aggcgccgga cgccttcagg ccgtgctcga
     6901 cggcgagggc gcagatcggc cggaccatcc gggcgcgctg ctcgggcgtg cagtcggcgg
     6961 tcgcctggct gaagctcggg accggttcgg cggggagcgg cccgggcagg tccggcagcc
     7021 ccgggtcctc cggctggcgc ttcgcggcct cgacggcgcg ctcgacgacc cgagccagcg
     7081 ccgcgtccga gaggtcgttg gtggtggcga cgcccgcgcg cctgccgacg atggcccgga
     7141 ctcggacctg gacgttccgc tcggcgacgt tctggtggat gccagagttg gcgaagcggg
     7201 tcagcgccga gtcgacgccg atgacgacca cctcggtctg atcggccctc gacgcgtcga
     7261 gcgcccgatc ggccaggctt tcgaagcgcg cacgcccgag tagctcgtcc atcggggccg
     7321 gctaccgcat cacgccgatc tggacgccgc gcacccggat cgcggcggcg ccgtgcccga
     7381 cgtgcatcgc
//
LOCUS       DASRIG010000312         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2771466, whole genome shotgun sequence.
ACCESSION   DASRIG010000312 DASRIG010000000
VERSION     DASRIG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771466"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFB52_08460"
     CDS             <1..931
                     /locus_tag="VFB52_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06405.1"
                     /translation="KLALLLAALALAAGLAACGSSDDSSDTTGTAETTSTGGEEAVTG
                     TFEPIAGVPQPYGDVSGEAELTRADGKTITAISLEGLVADTKYIAHLHTGGCGETDPG
                     GPHFKFNPQGGDEPPNEIHLSFTSNADGSGKAKVTADQEVPVGEAGSIVVHTDGDAMT
                     ASTATQQFASLFIHEGEEHEGDDHGDHDSGGEPGHDGGGIQPLSAKVACAELEGSSAE
                     TAEGPVPTIEVKGGEPVDGIQDLEFDAGEQIRFKVSGDEAEEIHVHGYDLSEEIPQGG
                     GTVEFDFPAEIEGIFEIELEEQAVQIAELQVNP"
     gene            928..>2071
                     /locus_tag="VFB52_08465"
     CDS             928..>2071
                     /locus_tag="VFB52_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HZO06406.1"
                     /translation="MITLPLAHALAARQDLPVPAWLFAWGASIVLIVSFFALAAAWQK
                     PRFERQNWRALGSGFSRWIVSLPVQVVCGLVGVFLLGVAIYAGLRGTEAPDRNFALTF
                     LFVTCWLGFPFFSALLGDVFRPFNPWRAVGRVAGAGFRLIAGQPPAHARYPEWLGRWP
                     AVVGLLAVVWLEVVYGSSGGVAVGLDPHAAAVAACVYTGYTLAGMAIFGVEEWCRKGE
                     IFSVYFGMFARLGAFGVEDGRLGRRRMLSASTSWATIPGSAALVIASIATTSYDGASE
                     GAFKGGIETVFGWLSDIGFGANTAIRVTDTIFMLLCFAGVALVYMVGVYGMRTVKGAP
                     SLEKLRTGFAHTLIPIALAYLIAHYFSLVVFQEQAQFTYLLSDPLGT"
BASE COUNT          320 a          741 c          675 g          335 t
ORIGIN      
        1 gaaactcgcg ctgctgcttg cggcgctggc gctggccgct ggcctcgcgg cctgcgggag
       61 cagcgacgac agcagcgaca ccaccggtac ggccgaaacg accagcacgg gcggcgaaga
      121 ggctgttacc ggcacgttcg agccgatcgc cggcgtcccg cagccctacg gcgacgtctc
      181 gggtgaagcc gagctgaccc gcgccgacgg caaaacgatc accgcgatca gcctcgaagg
      241 cctcgtcgct gacaccaagt acatagccca cctgcacacc ggtggctgcg gcgagaccga
      301 ccccggcggc ccgcacttca agttcaaccc gcagggcggc gacgagccgc cgaacgagat
      361 ccatctctcg ttcacctcga acgccgacgg tagcggcaaa gccaaggtca ctgccgacca
      421 ggaagtgccg gtgggcgaag cgggctcgat cgtcgtccac accgacggtg acgcgatgac
      481 cgccagcacc gccacccagc agttcgcttc gctcttcatc cacgaaggcg aggaacacga
      541 aggcgacgat cacggcgacc acgactccgg cggggagccc ggtcacgacg gtggcgggat
      601 tcagccgttg tccgccaagg tcgcctgtgc cgaactcgaa ggcagctccg ccgaaaccgc
      661 cgaaggcccg gttccgacga tcgaagtcaa aggcggcgaa ccggtcgacg gcatccagga
      721 cctcgaattc gacgccggcg aacagatccg cttcaaggtc agcggcgacg aagcggaaga
      781 gatccacgtc cacggttacg acctctccga ggagatcccg cagggcggcg gcacggtcga
      841 attcgacttc cccgccgaaa tcgaagggat cttcgagatc gagctcgagg agcaggcggt
      901 gcagatcgcc gagctgcagg tgaacccgtg atcacgctgc cgctggcgca cgcgctggca
      961 gccaggcagg acctcccggt ccccgcctgg ctgttcgcct ggggggcctc gatcgtcctg
     1021 atcgtctcct tcttcgcgct ggcggcagcc tggcaaaaac cccgcttcga gcgccagaac
     1081 tggcgcgcgc tcgggtcggg gttctcacgc tggatcgtct cgctgccagt ccaggtcgtc
     1141 tgcggccttg tcggcgtctt cctgctcggg gtcgcgatct acgccggcct gcgcggcacc
     1201 gaggcgccag accgcaactt cgccctcacc ttcctcttcg tcacctgctg gctcgggttc
     1261 ccgttcttct cggccctgct cggagacgtc ttccggccct tcaacccctg gcgcgcggtc
     1321 ggccgcgtcg ccggcgccgg tttccggctg atcgccggcc agcctcccgc gcatgcccgc
     1381 taccccgagt ggctgggccg ctggccggcg gtcgtcgggc tgctcgcggt cgtctggttg
     1441 gaggtcgtct acggctccag cggcggggtc gccgtcggtc tcgacccgca cgcggccgcc
     1501 gtcgccgcct gcgtctacac cggctacacg ctggccggca tggcgatctt cggggtcgag
     1561 gaatggtgca ggaagggcga gatcttctcg gtctacttcg ggatgttcgc ccggctcggt
     1621 gccttcgggg tcgaggacgg ccggctcggc cgccgtcgca tgctttccgc ctcgaccagc
     1681 tgggcaacga tccccggctc ggccgcgctg gtgatcgcct cgatcgcgac gacgagctac
     1741 gacggcgcct cagagggggc gttcaagggt gggatcgaga ccgtcttcgg ctggctcagt
     1801 gacatcggct tcggcgccaa caccgcgatc cgggtcaccg acacgatctt catgctgctc
     1861 tgtttcgccg gggtcgcgct cgtctacatg gtcggcgtgt acgggatgcg cacggtcaaa
     1921 ggcgccccct cgctagagaa actgcgcacc gggttcgccc acaccctgat cccgatcgcc
     1981 ctcgcctatc tgatcgccca ttacttcagc ctggtcgtct tccaggagca ggcccagttc
     2041 acctacctgc tctccgatcc gctggggacg g
//
LOCUS       DASRIG010000313         7517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1209888, whole genome shotgun sequence.
ACCESSION   DASRIG010000313 DASRIG010000000
VERSION     DASRIG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209888"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFB52_08470"
     CDS             complement(<1..635)
                     /locus_tag="VFB52_08470"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZO06407.1"
                     /translation="MTVELFNDEDFEPERPQKPKAPPPREPRKRGLRGETAKRILVAL
                     PWIVFAIAITVAGGIVFGLAMIAIGVVCLREYLGMTEDARPLAIPAYLAVAGLIVAAH
                     FGTAFNVLMVVAAIFPLLFLFAINTKQRDGITVSFSITLLGILWIGVPLAHAVFLRDL
                     PDHGAALLVDVLVGTFVADTAAYATGRMFGSHKIAPSISPNKTIEGLIGGFV"
     gene            complement(632..1300)
                     /gene="uppS"
                     /locus_tag="VFB52_08475"
     CDS             complement(632..1300)
                     /gene="uppS"
                     /locus_tag="VFB52_08475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZO06408.1"
                     /translation="MTAPRYVAIITDGNGRWAQQRGLPVIEGHRAGADVVKARLRDAA
                     ELGIEELTVFSFSTENWSRSEEEVGGLMAMFGERIAGETPELDEEGVRMRFIGRREGV
                     APELIEQMEWAEAKTAGNERITLFVAFNYGGRAEIVDAARSFQGDSEEEFRRHLYAPE
                     MHDPDLLIRTSGEQRISNYLLWQCAYSELVFSEALWPDFDRAAFEGALAEYETRQRRF
                     GART"
     gene            complement(1297..1860)
                     /gene="frr"
                     /locus_tag="VFB52_08480"
     CDS             complement(1297..1860)
                     /gene="frr"
                     /locus_tag="VFB52_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZO06409.1"
                     /translation="MELIDELLEDAKGRMAKSVESSRGELATVRTGRASPHLLDRIVV
                     DYYGTATPLKQLANVAASDARLLTVTPFDKSALGSIEKSIQESDVGLTPNNDGNVIRL
                     QIPEMTEERRREMVKVVHGVAEEGRVAIRNVRRDVMSDLRDLKKEGEAGEDDERRAEA
                     ALQKQTDEAIAEIDSLLKGKEEEILEV"
     gene            complement(1877..2617)
                     /gene="pyrH"
                     /locus_tag="VFB52_08485"
     CDS             complement(1877..2617)
                     /gene="pyrH"
                     /locus_tag="VFB52_08485"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZO06410.1"
                     /translation="MSDSAEPRFGRILLKLSGEALMGELEYGTDGAEVARIVRQVAAV
                     RERGVEVAIVVGAGNIYRGIDGASKGMDRATGDYMGMLATVLNALTLQDALESLGQYT
                     RVMSAIDVKEVAEPYIRRRAMRHLEKGRVVIFAAGTGNPFFTTDTAAALRALEIHAEA
                     ILMAKNGVEGVYDSDPATNPDAAFIPAITHREAIERGLKVMDSTALSLCMDNDLPIYV
                     FNMADEGNIDRIVCGEKVGTLVSSGPAA"
     gene            complement(2630..3235)
                     /gene="tsf"
                     /locus_tag="VFB52_08490"
     CDS             complement(2630..3235)
                     /gene="tsf"
                     /locus_tag="VFB52_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZO06411.1"
                     /translation="MSVSAADVKALRDRTGAAMMDCKAALSEADGDVDRAIELLRVKG
                     QASAAKREGRGTNEGVVASYIHANNRVGAMVEVQCETDFVARNEDFQVFAYQVALHVA
                     ATAPKYVTTDEIPEAEREAEKKVFEEKAREEGKPDNVVEKIVEGQLKKWGSEVALLDQ
                     THVNAEKHDSKTIEELRQELAAKTGENVRIARFSYFRVGEE"
     gene            complement(3232..4281)
                     /gene="rpsB"
                     /locus_tag="VFB52_08495"
     CDS             complement(3232..4281)
                     /gene="rpsB"
                     /locus_tag="VFB52_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZO06412.1"
                     /translation="MPEAGLKELLEAGVHFGHQTRRWNPNMRRFIFTELDGIHIIDLI
                     QTEALLENARRFAGELASGGGTVLFVGTKKQARGPVQEWAERCKMPYVNKRWLGGLLT
                     NFNTMSQRIARLHELNGWKEEGKLDLLPTKERMNMEAELAKLEFNLGGVRDMDRLPDA
                     VFVTDLKTEEIAVHEAARLRIPIIGLVDTNCDPTPVDYVIPGNDDAIRSCQLVIGTIG
                     GAVEEGSSAWRVQEEKRIAEEMERRKRDEEERKKREEEEKVRVEALEAKKAEEEAAAK
                     AAEAAKAAEAAKAAAEAPKASEPPKAPDVPAPSAPAAEQKAPAAQAAPAAAEPPKAEE
                     APATEAKDEKAEESK"
     gene            complement(4414..5094)
                     /locus_tag="VFB52_08500"
     CDS             complement(4414..5094)
                     /locus_tag="VFB52_08500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZO06413.1"
                     /translation="MPELELRIVHLDEALAVVDKPAGLVVHPAPSHSGPTLVDELAEI
                     LGGGGDPERPGIVHRLDKGTSGLLVVARDDAAHAALQEQVREREVERVYLALAGGRLG
                     SRTGTIDAPIGRASRQRHRMAVSGAASRAARTHFEVIELLPRETYLQAKLETGRTHQI
                     RAHFGAIGHPLTGDPTYGGAERYGLRRQFLHAHRLAFRHPVSGERLSFESPLPPDLAA
                     ALEAAREA"
     gene            complement(5087..5599)
                     /gene="lspA"
                     /locus_tag="VFB52_08505"
     CDS             complement(5087..5599)
                     /gene="lspA"
                     /locus_tag="VFB52_08505"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZO06414.1"
                     /translation="MSPAVKRAWLCAGLLAALVLVLDQVAKAIVEDQIVIGEQIEVLG
                     PLEFTLAHNRGVAFGIAGGAGAPLVLVTLFALGVVLFLFSRTPTRPWMWVAVGLLVGG
                     ALGNLVDRVRLGHVTDYIDLPPWPPFNLADVSITFGVILLVILYLFDTGAQARDATEA
                     KEGGAEGERA"
     gene            complement(5596..5898)
                     /locus_tag="VFB52_08510"
     CDS             complement(5596..5898)
                     /locus_tag="VFB52_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZO06415.1"
                     /translation="MIPLAISRGDVADYVSALFIVYTVLIFLNILISFVPRMPYRPWL
                     RAVLDFIKETTDPYLNFFRRFLPPLGGGGFALDLSPMVGVIVLLVARALIVGAIEG"
     gene            complement(5895..6635)
                     /locus_tag="VFB52_08515"
     CDS             complement(5895..6635)
                     /locus_tag="VFB52_08515"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HZO06416.1"
                     /translation="MVVGFCGSGNMAAAMARGWAGELGGMLFSDAGSGRARALAAELG
                     GEAVDNATLAERADLLVLAVKPAKLEEVAPQLGAAKKVVSILAGVPLERSRAAFPNAE
                     LLRVMPNVGVEVRKGVLCIAGEADAEVRDALAILGHVVEIADAEFDEATAIMSCAPAY
                     LALAVEALADAGAAAGLDRELAGDLVVETTAGTAELLRLRHPADVRKAVTSPGGSTEA
                     GLEALDREGARAAFEAAVAASLERMKGK"
     gene            complement(6686..>7517)
                     /locus_tag="VFB52_08520"
     CDS             complement(6686..>7517)
                     /locus_tag="VFB52_08520"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZO06417.1"
                     /translation="TETKAAGGLGSIAEQMVDHKVDVLLGGGRGRFTQTLTGGPDAGK
                     TVVESAQAQGYQYVTDATGLAAVTSKKKPLLGLFTNGNMSLEWSGPAASLGKGNAPVP
                     CDENKRPAGEPSLAAMTDKAIALLKGDPEGFFLQVEGASIDKQDHATNACGQLGETVA
                     FDEAIGIALDYQETHQNTLVIVSADHSHTSQIVAEDSSGTGIPTGYSTNLVTKEGSTL
                     SLTYGTAGYGGEGKVPVSPSSASQQHTGAVVPVWAVGPGALNVLGTNDHTDLFTTLGG
                     "
BASE COUNT         1019 a         2614 c         2579 g         1305 t
ORIGIN      
        1 acgaagccgc cgatcaggcc ctcgatcgtc ttgttgggcg agatgctggg ggcgattttg
       61 tggctgccga acatgcggcc ggtcgcgtag gcggcggtgt cggcgacaaa ggtgccgacc
      121 aggacgtcga ccagcagcgc ggcgccgtgg tcgggcaggt cgcggaggaa gacggcatgg
      181 gcgagcggaa cgccgatcca gaggatccca agcagggtga tgctgaacga gaccgtgatc
      241 ccgtcgcgct gcttggtgtt gatcgcgaac aggaagagca gcgggaagat cgcggcgacg
      301 accatcagca cgttgaaggc ggtgccgaag tgggcggcga cgatcagccc ggcaaccgcg
      361 aggtaggcgg ggatcgcgag cgggcgcgcg tcctcggtca tgcccaggta ctcgcgcagg
      421 cagacgacgc cgatcgcgat catcgccagg ccgaagacga tcccgccggc aaccgtgatc
      481 gcgatcgcga agacgatcca gggcagcgcc accaggatcc gcttcgcggt ctccccgcgc
      541 agcccgcgtt tgcgcggctc gcgcggcggc ggcgctttcg gtttctgcgg gcgctccggc
      601 tcgaagtcct cgtcgttgaa cagttcgacg gtcacgtgcg cgctccaaag cggcgctggc
      661 gggtctcgta ctcggccagg gcgccctcga aggcggcgcg gtcgaagtcg ggccagagcg
      721 cctcgctgaa gaccagctcc gagtaggcgc actgccagag caggtagttg gagatccgtt
      781 gctcgccgct ggttcttatg agcaggtctg ggtcgtgcat ctcaggcgcg tacaggtggc
      841 ggcggaactc ctcctccgag tctccctgga agcttcgcgc cgcgtcgacg atctccgccc
      901 tgccgccgta gttgaaggcg acaaacagcg tgatccgctc gttgccggcg gttttcgctt
      961 ccgcccactc catctgctcg atcagctccg gcgcgacccc ctcgcggcgg ccgatgaagc
     1021 gcatccgcac gccttcttcg tcgagctcgg gggtttcgcc ggcgatccgc tcgccgaaca
     1081 tcgccatcag gccgccgacc tcctcctcgg agcggctcca gttctcggtc gagaaggaga
     1141 acaccgtgag ctcctcgatc cccagctcgg cggcgtcgcg caggcgtgcc ttgaccacgt
     1201 cggcgcctgc ccggtgcccc tcgatgaccg gaaggccccg ctgctgcgcc cagcggccgt
     1261 tcccgtcggt gatgatcgcg acgtaccgcg gggcggtcaa acctcgagga tttcctcttc
     1321 cttgcccttg agcagcgagt cgatctcggc gatcgcctcg tcggtctgct tctgcagcgc
     1381 ggcctcggcg cggcgctcgt cgtcctcgcc ggcttcgccc tccttcttca ggtcgcgcag
     1441 gtccgacatc acgtcgcggc gcacgttgcg gatcgcgacg cggccctcct cggcgacccc
     1501 gtgcacgact ttgaccatct cgcgacggcg ctcctcggtc atctccggga tctgcagccg
     1561 gatcacgttg ccgtcgttgt tcggcgtcaa gccgacgtcg gactcctgga tcgacttctc
     1621 gatcgacccc agggcgctct tgtcgaaggg cgtcacggtg agcaggcggg cgtcgctggc
     1681 ggcgacgttg gcaagctgct tgagcggcgt cgccgtgccg tagtagtcga ccacgatgcg
     1741 gtcgagcagg tgcggcgagg cgcggccggt gcggaccgtg gccagctcgc cacggctcga
     1801 ctcgaccgac ttggccatgc gccccttcgc gtcctcgaga agttcgtcga tcagctccac
     1861 ggatccgttc ctcgcttcat gcggccggcc cgctggagac cagcgttccc accttctcgc
     1921 cgcagacgat cctatctatg ttgccctcgt cggccatgtt gaagacgtag atgggcagat
     1981 cgttgtccat gcagagcgag agcgcggtcg aatccatcac tttcagccca cgctcgatcg
     2041 cttcgcggtg ggtgatcgcc gggatgaagg cggcgtcggg attggtcgcc gggtcggagt
     2101 cgtagacccc ctccaccccg ttcttcgcca tcaggatggc ctccgcatgg atctccagcg
     2161 cgcgcagggc cgccgcggtg tcggtggtga agaacgggtt gccggtgccg gcggcgaaga
     2221 tcaccacgcg gcccttctcc aggtggcgca tcgcgcgccg gcggatgtag ggctcggcga
     2281 cctccttgac gtcgatcgcc gacatcaccc gcgtgtactg gccgaggctc tccagcgcgt
     2341 cctgcagggt gagcgcgttc agcaccgtcg ccaacatccc catgtagtcg ccggtcgccc
     2401 ggtccatgcc tttcgaggcg ccgtcgatcc cgcggtagat gttgccggca ccgaccacga
     2461 tcgcgacctc gacgccgcgc tcgcggaccg ccgccacctg gcgcacgatc cgcgccacct
     2521 cggcgccatc ggtgccgtac tccagctcgc ccatcagggc ctcgccggaa agcttcagca
     2581 ggattcgccc gaatcgtggt tcggcagagt cgctcactta cccgtgaccc tattcctcgc
     2641 cgacgcggaa ataggagaac cgggcgatcc ggacgttctc gccggttttc gcggcgagct
     2701 cctggcgcag ctcctcgatc gtcttcgagt cgtgtttctc ggcgttgacg tgcgtctgat
     2761 cgagcagcgc gacctcgctg ccccatttct tcagctggcc ctcgacgatt ttctcgacca
     2821 cgttgtcggg cttgccttct tcgcgcgcct tctcctcgaa gaccttcttt tcggcctcgc
     2881 gctcggcctc ggggatctcg tccgtggtga cgtacttcgg cgcggtcgcc gccacgtgca
     2941 gggcgacctg gtaggcgaag acctggaagt cctcgttgcg ggcgacgaag tcggtctcac
     3001 actggacctc gaccatcgcg ccaaccctgt tgttggcgtg gatgtaggag gcgacgacgc
     3061 cctcgttggt gccgcgacct tcgcgcttgg cggccgaggc ctgccccttc acgcgcagca
     3121 gctcgatcgc gcggtcgacg tcgccgtcgg cctcgctcaa ggctgctttg cagtccatca
     3181 tcgccgcccc ggtccgatcc cggagcgcct ttacgtcggc agcgctaacg ctcatttcga
     3241 ctcctcagct ttctcgtctt tcgcctcggt ggccggagcc tcctcagctt taggcggctc
     3301 ggcggcagcc ggcgctgcct gggccgccgg ggctttctgc tcagccgcgg gcgcggacgg
     3361 tgccggaaca tctggcgctt tgggaggctc cgaggctttc ggcgcctcgg cggcggcttt
     3421 tgcggcctcc gccgcttttg ctgcctcggc agctttcgcc gcggcctctt cttcggcttt
     3481 tttcgcctcc agcgcttcga cgcggacttt ctcttcttcc tcgcgtttct tgcgctcttc
     3541 ctcgtcgcgt ttgcgccgct ccatttcctc ggcgatccgt ttctcctcct gcacgcgcca
     3601 ggcggaggag ccctcctcga cggcgccgcc gatcgtgccg atcaccagct ggcaggagcg
     3661 gatcgcgtcg tcgttgccgg ggatcacgta gtcgaccggg gtcgggtcgc agttggtgtc
     3721 gaccaggccg atgatcggga tccgcaggcg tgccgcctcg tggaccgcga tctcctcggt
     3781 tttcaggtcg gtgacgaaga ccgcgtcggg cagccggtcc atgtcgcgga cgccgccgag
     3841 gttgaactcg agcttcgcga gttcggcctc catgttcatc cgctccttgg tcggcaggag
     3901 gtcgagtttg ccctcctctt tccagccgtt gagctcgtgc aggcgcgcga tccgctgcga
     3961 catcgtgttg aagttggtca gcagcccgcc cagccagcgt ttgttgacgt agggcatctt
     4021 gcagcgttcg gcccactcct ggaccgggcc gcgggcctgc ttcttggtgc cgacgaagag
     4081 caccgtgccg ccgccgctgg cgagctcgcc ggcgaagcgg cgcgcgttct ccagcagcgc
     4141 ctcggtctgg atcaggtcga tgatgtggat cccgtccagc tcggtgaaga tgaaccggcg
     4201 catgttcggg ttccagcggc gcgtctggtg gccgaagtga actccggcct cgagcagctc
     4261 tttcaggcct gcctctggca tttgaatctc cttggtttgg gtactacggc cccgctccgc
     4321 ttgccgggac ccggcgcgtc tttgcgccga gcaggatccg gttcgcttgc gaacgggggt
     4381 tggtttctga acagctgatt gtagaggggc gcgctaggcc tcgcgggccg cctccagcgc
     4441 ggccgcgagg tccggcggca gcggcgactc gaagctcagc cgctcgccgc tgaccgggtg
     4501 acggaaggcg agccggtggg cgtgcaggaa ctggcgccgc aggccgtagc gctcggcgcc
     4561 cccgtaggtg gggtcgccgg tcagcgggtg cccgatcgca ccgaagtggg cgcggatctg
     4621 gtgcgtgcgg ccggtctcca gcttcgcctg caggtaggtc tcgcgcggca acagctcgat
     4681 cacctcgaag tgggtgcggg ccgcgcgcga ggcggcgccg gagaccgcca tccggtggcg
     4741 ctggcgcgag gcgcggccga tcggcgcgtc gatcgtgccg gtgcgggagc cgagcctccc
     4801 cccggcgagc gccaggtaga cccgttcgac ctcgcgctcg cgcacctgct cctgcagcgc
     4861 cgcgtgagca gcatcgtcgc gggcgacgac gagcagcccg ctggtcccct tgtcgagccg
     4921 gtgcacgatc cccggccgct ccgggtcacc gccgccgccg aggatctcgg cgagttcgtc
     4981 gaccagggtc gggccgctgt gcgagggagc cgggtgcacg accagcccgg ccggcttgtc
     5041 gacgacggca agcgcctcgt cgaggtggac gatccgcagc tccagctcag gcacgctctc
     5101 cctccgcccc accctccttc gcctccgtcg cgtcccttgc ctgcgccccc gtgtcgaaca
     5161 ggtagaggat cacgagcagg atcacgccga aggtgatcga gacgtcggcg aggttgaacg
     5221 gtggccaggg tggcaggtcg atgtagtcgg tgacgtggcc gagccgcacc cggtcgacca
     5281 ggttgccgag cgcgccgccg accaggagac cgaccgcgac ccacatccag ggcctggtcg
     5341 gtgtgcgcga gaagaggaag aggacgacgc cgagcgcgaa cagggtgacc aggaccagcg
     5401 gcgcccccgc cccgccggcg atcccgaagg cgacgccgcg gttgtgggcg agcgtgaatt
     5461 cgagtgggcc gagcacctcg atctgctcac cgatcacgat ctggtcctcg acgatcgcct
     5521 tcgctacctg gtcgagcacg agcaccagcg cggcgagcag gcccgcgcag agccaggcgc
     5581 gcttgaccgc ggggctcacc cctcgatcgc gccgacgatc agggcacgcg cgaccaagag
     5641 cacgatcacg ccgaccatcg ggctgaggtc gagggcgaag ccgccaccgc cgagcggcgg
     5701 caggaagcgg cggaagaagt tcaggtaggg gtcggtcgtt tctttgatga agtcgagcac
     5761 cgcgcgcagc cagggccggt agggcatccg cggcacgaac gagatcagga tgttgaggaa
     5821 gatcaggacc gtgtagacga tgaacagggc gctgacgtag tcggcgacgt cgccacggct
     5881 gatcgcgagt gggatcactt gcccttcatc ctctccaatg aggcggctac cgcggcctcg
     5941 aacgcggccc gagcgccctc gcggtcgagc gcttccaggc ccgcttcggt gctgccgccc
     6001 ggcgaggtga ccgccttgcg gacgtcggcc gggtggcgca gccgcagcag ctcggcggtg
     6061 ccggcggtgg tctcgaccac caggtcgccg gccagctcgc ggtcgagccc ggcggcggcg
     6121 cccgcgtcgg ccaaggcctc gaccgcgagc gccaggtaag cgggcgcgca gctcatgatc
     6181 gcggtggcct cgtcgaactc ggcgtcggcg atctcgacca cgtggccgag gatggcgagc
     6241 gcatcgcgca cctcagcgtc agcctcgccg gcgatgcaga gcacgccctt gcgcacctcg
     6301 accccgacgt tcggcatcac ccgcagcagt tcggcgttcg ggaaggccgc ccgcgagcgc
     6361 tccagcggca ccccggcgag gatcgagacg accttcttgg cggccccgag ctgcggcgca
     6421 acctcctcca gcttcgccgg cttcaccgcc agcaccagca ggtcggcgcg ctcggccagg
     6481 gtcgcgttgt cgaccgcctc gccgccgagc tctgcggcca gcgcccgcgc ccggcccgag
     6541 ccggcgtcgg agaacagcat gccgcccagc tcgccggccc agccgcgggc catcgccgcg
     6601 gccatgttcc cggatccaca aaaaccaacg accatggccc gggagactat ccccgggcca
     6661 tggtcgcgga ccgtccgccg gggctttagc cgcccagcgt cgtgaacagg tccgtgtggt
     6721 cgttggtccc gagcacgttc agcgcgccgg ggccgacagc ccagaccggc accacagcgc
     6781 cggtgtgctg ctggctggcc gaggacggcg agaccggcac tttgccttcg ccgccgtagc
     6841 cggcggtgcc gtaggtgagg ctgagggtgc tgccttcctt ggtcaccagg ttggtcgagt
     6901 agccggtcgg gatcccggtc ccgctgctgt cctcggccac gatctggctc gtgtgcgagt
     6961 gatcggcgct gacgatcacc agggtgttct ggtgggtctc ctggtaatcg agggcgatgc
     7021 cgatcgcttc gtcgaaggcg accgtctcgc cgagctgacc acaggcgttc gtggcgtggt
     7081 cctgcttgtc gatcgaggcg ccctcgacct ggaggaagaa gccctccggg tcgcctttca
     7141 gcagcgcgat cgccttgtcg gtcatcgccg ccagactcgg ctcgccggcc ggccgtttgt
     7201 tctcgtcgca ggggacgggc gcgttgccct tgcccagcga agcggccgga ccgctccact
     7261 ccagcgacat gttgccgttg gtgaagaggc cgaggagcgg tttctttttg ctggtcacgg
     7321 ccgccagccc ggtggcgtcg gtgacgtact ggtagccctg cgcctgggcg gattcgacca
     7381 ccgttttgcc cgcgtcgggg ccgccggtga gcgtctgggt gaagcggccg cggccgccac
     7441 cgaggagcac gtcgactttg tggtcgacca tctgctcggc gatcgagccg agaccgccgg
     7501 cggccttggt ctcggtc
//
LOCUS       DASRIG010000314         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1465096, whole genome shotgun sequence.
ACCESSION   DASRIG010000314 DASRIG010000000
VERSION     DASRIG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465096"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1083)
                     /locus_tag="VFB52_08525"
     CDS             complement(55..1083)
                     /locus_tag="VFB52_08525"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZO06418.1"
                     /translation="MAGDGYRVGVLGATGLVGTTILELLASRGFPASEVRPLASARSA
                     GKEIEWGGKMLTVREIGDESIQGLDLVLSSAGGSVSAEWAPKIVEAGAVVVDNTSHFR
                     MRDDVPLVVSEVNPEALAAHNGIVANPNCSTMQMVVALKPLYDAAGIERLVVSTYQAV
                     SGTGKAAIDELLEQSHAILHGQEASASIYPHQIAFNPLAQAGSFADGDDHTDEERKLI
                     NETRKILGDAEIKVSATCIRVPVVTGHSEAVNVQTREPLSADRARELLAAAPGITVVD
                     DPANSRFPMAIEAAGKDDVFVGRIREDASQASTLDLWVVADNLRKGAATNAVQLAELL
                     VERELLSS"
     gene            complement(1101..>2303)
                     /locus_tag="VFB52_08530"
     CDS             complement(1101..>2303)
                     /locus_tag="VFB52_08530"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZO06419.1"
                     /translation="RIVKAREQGHHVVAVLSARGKTTDELIASAEEVSTSPDPRELDM
                     LLSTGERISCALCAMAIHDMGHSAISLTGSQAGIVTDSTHTKARIIDVRADRISDALG
                     EDRIVLVAGFQGVSTDSRDVTTLGRGGSDTTAVALAAALRADVCEIYTDVAGVFSADP
                     RIVPSARKLTQVSFEEMLEMSASGAGVLQLRSVEYARNHGVKIHCRSSFEDGPGTLVV
                     AEDQTMENPFVTAVTHSNAEARVTLTGVRDEPGVAGRIFGALAEANVNVDVIIQNEPV
                     GGDQLADLSFTVDRADLGTAKDTIAALGDVSLKVRTDEQIGKVSIIGAGMRSHPGVAA
                     KVFEVLGEERINIEMISTSPIKISCVIPADRVSDAVNALHKTFELGDDAIRPEDPTGP
                     SHRPTVAG"
BASE COUNT          312 a          763 c          851 g          377 t
ORIGIN      
        1 ttcgtcttgc gccgcctcag cggcggccaa tcgacttcgt caaccagagg ctgactaact
       61 cgacagcagc tcgcgctcga ccagcagctc ggcgagctgg accgcgttcg tagcggcgcc
      121 cttgcgcagg ttgtcggcga ccacccagag gtcgagggtg ctcgcctgcg aggcgtcctc
      181 gcggatccgg ccgacgaaga cgtcgtcctt gccggccgcc tcgatcgcca tcgggaagcg
      241 cgagttggcc gggtcgtcga cgacggtgat gccgggcgcg gcggcgagca gctcgcgggc
      301 gcggtcggcg ctcagcggct cgcgggtctg cacgttgacc gcctcggagt ggccggtgac
      361 gaccgggacc cggatgcagg tggcgctgac cttgatctcg gcgtcgccga ggatcttgcg
      421 ggtctcgttg atcagcttgc gctcctcgtc ggtgtgatcg tcaccgtcgg cgaagctgcc
      481 cgcctgggcg agcgggttga aggcgatctg gtgcgggtag atcgaggcgc tcgcctcctg
      541 gccgtggagg atcgcgtgcg actgttcgag cagctcgtcg atcgccgcct tgccggtccc
      601 ggagaccgcc tggtaggtgg agacgacgag gcgctcgatc ccggccgcgt cgtagagcgg
      661 cttcagcgcg acgaccatct gcatggtcga gcagttcggg ttggcgacga ttccgttgtg
      721 ggcggccagc gcctccgggt tgacctcgga gacgaccagc ggcacgtcgt cgcgcatgcg
      781 gaagtggctg gtgttgtcga cgaccacggc accggcctcg acgatcttcg gcgcccactc
      841 ggcgctgacc gagccgccgg ccgaggagag caccaggtcg agcccttgga tcgactcgtc
      901 gccgatctcc cgcacggtca gcatcttgcc gccccactcg atctctttgc cggccgagcg
      961 agcggacgcc agcgggcgca cctcgctggc ggggaagccg cgcgaagcga gcaactcaag
     1021 gatcgtcgtc ccgaccagcc cggtcgcgcc cagtactccg actctgtatc cgtcgcccgc
     1081 catcgtcgtg tcgggcgggc tcagccggcg accgtcggcc ggtgactggg gccggtcggg
     1141 tcctcgggcc ggatcgcgtc gtcgccgagc tcgaacgtct tgtgcagggc gttgaccgcg
     1201 tcggagacgc ggtcggccgg gatgacgcac gagatcttga tcggcgaggt ggagatcatc
     1261 tcgatgttga tccgttcctc gccgagcacc tcgaagactt tggcggcgac gccggggtgc
     1321 gagcgcatcc ccgcgccgat gatcgagacc ttgccgatct gctcgtcggt gcggactttc
     1381 agcgagacgt cgccgagcgc ggcgatcgtg tccttggcgg tgccgaggtc ggcgcggtcg
     1441 acggtgaacg agaggtcggc gagctgatcg cccccgaccg gctcgttctg gatgatcacg
     1501 tcgacgttga cgttcgcctc ggcgagcgcg ccgaagatgc ggccggcgac gccgggctcg
     1561 tcgcggacac cggtcagcgt gacgcgggcc tccgcgttgg agtgggtgac ggcggtgacg
     1621 aaggggttct ccatggtctg atcctcggct acgacgaggg taccgggacc gtcctcgaag
     1681 ctgctgcggc agtggatctt caccccgtgg ttgcgcgcgt actcgaccga gcgcagctgc
     1741 agcacaccgg cccccgaggc cgacatctcg agcatctcct cgaaggagac ctgggtcagc
     1801 ttgcgggcgc tggggacgat ccgcggatcg gcgctgaaga cgccggcgac gtcggtgtag
     1861 atctcgcaga cgtcggcgcg cagcgccgcg gccagcgcca ccgcggtggt gtcggagccg
     1921 ccgcggccga gcgtggtcac gtcgcggctg tcggtggaga cgccctggaa gccggcgacc
     1981 agcacgatcc ggtcctcgcc gagcgcgtcg gagatgcggt cggcgcgcac gtcgatgatc
     2041 cgcgccttgg tgtgggtcga gtcggtgacg attcccgcct gcgagccggt cagcgagatc
     2101 gccgagtggc ccatgtcgtg gatcgccatc gcgcagagcg cgcaggagat ccgctccccg
     2161 gtggagagca gcatgtccag ctcgcgcggg tcggggctgg tcgagacctc ctcggccgag
     2221 gcgatcagct cgtcggtcgt cttgccgcgc gcggagagca cggcgacgac gtgatggccc
     2281 tgctcgcgcg ccttcacgat gcg
//
LOCUS       DASRIG010000315         9262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2773066, whole genome shotgun sequence.
ACCESSION   DASRIG010000315 DASRIG010000000
VERSION     DASRIG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773066"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFB52_08535"
     CDS             complement(<1..830)
                     /locus_tag="VFB52_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06420.1"
                     /translation="MLIEILVSAMVLTIASAGVILLLQTTTKTQAEQRNSSEAYALAQ
                     EDQARLTSMRLATLNRLDQTRTVTLNKTQFQVRSRGVFVNDSTSIPSCGEGTSSADYV
                     EITSSVTWNGMDPGEKAKIVSVLSPSNGSLDPNHGTIAFSVVTQTQTPLPNVYVSGGN
                     GAFAGYTDALGCAVFTDLPAGNYTASVIGGPGLVGHDGESTQSEVVPVAGGDTKTVTF
                     EFDSPGAIKVPFKFRVGSSGSYLPSSQPAIVAYNQGMSAAKVFWTSSGSPEATVTASN
                     L"
     gene            1029..1382
                     /locus_tag="VFB52_08540"
     CDS             1029..1382
                     /locus_tag="VFB52_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06421.1"
                     /translation="MSAESNSTCPVCRTAEVVCGKWTLLLIRDLAAGNSRFCELERSL
                     EGISPRTLSLRLRALEEEGIVERHTYPEVPPRVEYALTAKGEALVPLIDDMRSYGTRW
                     LLGEDADVAEPAAAI"
     gene            1458..3155
                     /locus_tag="VFB52_08545"
     CDS             1458..3155
                     /locus_tag="VFB52_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06422.1"
                     /translation="MRDFLLNEALKRLATEASTRLATLVASGDEIPFDVGADGGEETP
                     FYSYVPMTGRFVEERADELRALPGFAGAREAAVEAGVAAPYLEARGEQVAADPGERAE
                     RMLIVFFAELWEGSNAFALDRERLEDALSALDAESRDADDADVLIVPIVGLRMSVQRL
                     QLPHGVRIVRADSIEAPIDAMRSEGMGRAAWEPQFLAVAEQDADGGTDDALAQLRELI
                     SVMRLFKGGGVGLGPYAFAPTGADNWRRVVTGVPQTRPGGYRLGEEEAAELSEFASTL
                     ESRPDPDGALTWAVGRFEMGCERESALEGLSDHLLALRAVLEGHGPVGASLPMRAAAL
                     ISDEQLDRIEAREKVEEVLHLEREMMNGRSLPNAIELAGWVEEGVRRLLRQAALGELG
                     NDLIGTADETLIANGLAEGDAEITISVAEDFPEPVIPDEPLNESEPVIEQLAIPEPPA
                     AFEEPAEFRIEHVPPAPEATEQEDDEGMEQETRIMEPIPAEGEIKVTAMPWLDEVSVA
                     RPGDSLEWPAPSNEIERDLQHRERIDTPRVRHLFPVPDSDWEVSHLEFEYSRRGGMS"
     gene            complement(3172..3810)
                     /locus_tag="VFB52_08550"
     CDS             complement(3172..3810)
                     /locus_tag="VFB52_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZO06423.1"
                     /translation="MAAVLACGTGAALSHQSAAYLWGFLKPEGAPVHVTSPSMSGKVS
                     QRGIVLHRSPSLKREGEVIRRERIPVTTPRRTIVDLEQTIEPWKARRAKKQAEFMGFE
                     LALPTDRTRSDLEIDFLGFLSKHGFPAPEVNADVAGYEVDFLWRSERVVVETDFFDYH
                     RGHQAFEDDHKRELHLRRAGFAVRRYTGAQLDNYPAEIVVDLGEVLGRRNRP"
     gene            complement(4062..5213)
                     /locus_tag="VFB52_08555"
     CDS             complement(4062..5213)
                     /locus_tag="VFB52_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HZO06424.1"
                     /translation="MPTLGEVLEGKEICICAGSGGVGKTTTSAAIATGMAAQGLKVCV
                     LTIDPAKRLADSLGLKELGNEARRVDPALFEKQGVEMKGELWAMMLDPKQTFDELIAR
                     QAPDEESRDRVLENRIYQQISSALAGSQEYMAMEKLFELHTEGDFDLLVLDTPPTRNA
                     LDFLDAPKRLTQFIEGKSLRMFMKPTGLAAKVAGRGASVALGIFKRIVGFDLLADLAE
                     FFNAFSGMVDGFQARAKRVNKLLADPSTCFLVVCGPQGEPIDEAVYFHRKLVEAKLPF
                     GGVIVNKVHYPAEKLRGEVDDLPTSLTEKLGDEDLAQRVAANFSDYQALAERDARNIK
                     HLASELRTKGVIRVPYLDEDVHDLAGLAEINRYLFATGEERVAMAAGLV"
     gene            complement(5213..6106)
                     /locus_tag="VFB52_08560"
     CDS             complement(5213..6106)
                     /locus_tag="VFB52_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HZO06425.1"
                     /translation="MIVTGKGGVGKTTVAVALGLRAAAEGKRTIVCEVSSQENTSRMF
                     EHTAVGFHEVEMEENLWSISIDPDESMREYVLLQLKVRAMRDMLFRSRIFNYLAAATP
                     GLKELVTIGKIWELAQLDRKVKKGRKYDLVIVDAPATGHGVGFLQTPRTFASIARVGP
                     IHSQAQTLDRFITDHEHTGTAIVALPEEMPVNESAALEKDLRDEVGVAVDRVYLNGLY
                     PERFSKAEAEKLAKVAEAEDGAARAAARAALSEHGRARSQRAQLARLRRRVEAPVKTL
                     PFLFEPELGVEAARRLSKRLG"
     gene            6199..7374
                     /locus_tag="VFB52_08565"
     CDS             6199..7374
                     /locus_tag="VFB52_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO06426.1"
                     /translation="MGFLSRKKKTAPVSVEEQHPIVEELQEMEEVVPEYAHEEPKSAP
                     VAVEDDYSDEYEAEEEDDYAEEPVEAEPAPEPEPVAEPAPVVRVAEPVAEVREEPVAE
                     VAEEEEDDGRLKAADLLAPMRQDFVEERRAAEQAPVKSAEVIAFANQKGGVAKTTTTL
                     NLAVAFAESGYRVLCIDLDPQGNLTMSQGIDPDKVEKSLYDVLVNDMPISEIIMRREI
                     DIAVASIDLAGAEIAMSTKIGRERSLEKALKEVEGDYDFVCIDTPPSLGLLTINALTA
                     ANKVIVPVQCEYLSMRGLVQLQNTLKMIQENLNPDVRIEGILPTMLDSRTVHAKEAVE
                     ILEENFGDLVFQSRIKKAIKFAEAPVKGASVLKYDSGSNAANYYRELAKEVLANGSA"
     gene            7388..8869
                     /gene="ftsZ"
                     /locus_tag="VFB52_08570"
     CDS             7388..8869
                     /gene="ftsZ"
                     /locus_tag="VFB52_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZO06427.1"
                     /translation="MREGPLADLFRSTADDAPQDPPEAPRYGRQDPAEAKKAEEAAAT
                     EQPAAAAPAPPPEPPAPEPTPEPEPPAREPEPAPPQPVADAPYDHASEESSAASAPER
                     LKRVFAEEPRPRDREDFLPGSPRYGREEPAYEPPGSGEPHQPVIKVVGVGGAGVNAVN
                     RMVDARIPGVEFMAVNTDLQSLQLCVADVTVHIGEEQTRGLGAGADPEVGHRSAFEEQ
                     DKIKRLLKGSDMVFVAAGAGGGTGSGAAPVVARLAREVGALTVGIVTKPFSFEGSRRG
                     TQAEKGIEELAAEVDTLIVVPNDRLLQVLDQQTSMVEAFQVADDVLRQGVQGISDLVT
                     LPAVINLDFADVRTIMSDAGRALLGIGMAYGDDRAILAAEKAISSPLLETSMEGARSI
                     LLSITGGSDLSLVEVSEAAKVVGEAAHPEANIIFGANVDEELTDQIWVTVVATRFDGR
                     PSTPRRSSEPTIRRTKDAPAERRSGRGERGGDLGIDVPEFLPS"
     gene            8882..>9262
                     /locus_tag="VFB52_08575"
     CDS             8882..>9262
                     /locus_tag="VFB52_08575"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZO06428.1"
                     /translation="MGGVVAAGHPLSAEAGAEVLREGGNAVDAAIGAVLASFAVESPL
                     TGFGAGGYMLVHREAPGGVPGSGRKESDSDAPSAGVTEQTLIDFFVAAPGLDGIERRA
                     ELVPVPVHFDAETSQTFFVGPASCG"
BASE COUNT         1466 a         3195 c         3149 g         1452 t
ORIGIN      
        1 aggttgctcg cggtgaccgt ggcttccggg ctcccacttg aggtccagaa gaccttcgcc
       61 gccgacatcc cctggttgta ggcgacgatt gcgggctggc tgctcggcag atagctgccg
      121 ctgctgccga cccggaattt gaacgggact ttgatcgcgc ccgggctgtc gaactcgaag
      181 gtgaccgtct tggtgtcgcc gccggcgacg ggaacgactt cggactgggt ggattcgccg
      241 tcgtgtccga ccaggcccgg gcctccgatg accgacgccg tgtagttgcc ggccgggagg
      301 tcggtgaaga ccgcgcaccc gagtgcgtcg gtgtatccgg cgaaggcgcc gttgccgccg
      361 ctgacgtaga cgttcgggag cggcgtttgg gtctgggtga cgaccgagaa ggcgatcgtc
      421 ccgtggttcg ggtcgagcga gccattcgag ggcgagagga cgctgacgat cttcgccttt
      481 tcgccggggt ccatcccgtt ccaggtgacg ctcgaggtga tctcgacgta gtcggccgat
      541 gaggtcccct cgccacacga ggggatggag gtggagtcat tgacgaagac gccgcgggag
      601 cggacctgga actgcgtttt gttcagcgtc accgtcctcg tctggtcgag tcggttgaga
      661 gtggcgagcc gcatcgaggt gaggcgggcc tggtcttcct gggcgagcgc gtaggcctcg
      721 ctgctgttcc gctgctcggc ctgcgtcttc gtcgtcgtct gcaggagcag gatcacgccg
      781 gccgaggcaa tggtcaggac catcgcgctg accaggatct cgatcagcac gaagccctct
      841 tcgcgagcgt ggttctgagc agggcggagc ctcactgggt tcccatcgga tcggctcccc
      901 cggcccttta accgccgcca cccacttttc aggaccccag ctgggatccg ccattgcttc
      961 agttagtgaa gccatgtggc tacacttgtt gaagcaagcg gttcacccca ccgcagagga
     1021 atgtcgcaat gagcgccgag agcaactcga cctgtcctgt ctgtcgcacc gccgaagtcg
     1081 tctgcggcaa gtggaccctt ttgctgatcc gcgacctcgc cgccgggaac agccgctttt
     1141 gcgagctgga gcgcagtttg gaggggatca gcccgcgcac cctctcgttg cgcctgcggg
     1201 cgctggagga ggagggcatc gtcgagcgcc acacctaccc cgaggtgcca ccgcgggtcg
     1261 agtacgcgct caccgccaaa ggcgaggccc tggtcccgct gatcgacgac atgcgcagct
     1321 atgggacccg ctggctactc ggcgaggacg ccgacgtggc tgagcccgcc gccgcgatct
     1381 aggtcaaacg tccagtcggt gcctgcaagc ctgcgtgcag gcgcaggagg gagggccggg
     1441 gggcggaagc ctcaggcgtg cgtgacttcc tcctcaacga agccctgaag cgcctcgcga
     1501 ccgaggcctc gacccggctc gcgaccctgg tcgccagtgg cgacgagatc cccttcgacg
     1561 tcggagccga cggcggcgag gaaaccccct tctacagcta cgtgccgatg accggccgtt
     1621 ttgtcgagga gcgcgccgac gagctgcgcg ccttgcccgg cttcgccggc gcccgcgagg
     1681 cggcggtcga ggccggcgtc gccgcccctt atctggaggc gcgcggcgag caggtcgccg
     1741 ccgaccccgg cgagcgcgcc gagcgcatgc tgatcgtctt cttcgccgag ctctgggagg
     1801 gctcgaacgc gtttgcgctc gaccgcgagc ggctcgagga cgcgctctcc gcgctcgacg
     1861 ccgagagccg tgacgccgac gacgccgatg tcctgatcgt gccgatcgtc ggcctgcgga
     1921 tgtcggtgca gcggctgcaa ctgccgcacg gcgtccgcat cgtgcgcgcc gactcgatcg
     1981 aggccccgat cgacgcgatg cgcagcgagg gaatgggaag ggcggcctgg gagccgcagt
     2041 tcctcgctgt ggccgagcag gacgccgacg gcggtaccga cgacgcgctc gcgcagctgc
     2101 gcgagctgat cagcgtgatg cgcctgttca aaggcggcgg cgtcggcctc gggccctatg
     2161 ccttcgcccc gaccggcgcc gacaactggc gccgcgttgt caccggcgtg ccccagaccc
     2221 gcccgggcgg ctaccgcctc ggcgaggagg aggccgcgga gctttctgag ttcgcgagca
     2281 ccctggagtc gcgccccgat cccgacggcg ccctcacctg ggcggtcggc cgcttcgaga
     2341 tgggctgcga gcgcgaaagc gcgctggagg ggctgagcga ccatcttttg gctctgcgag
     2401 cggtgctcga gggccatggc ccggttggcg catcgctgcc gatgcgcgcc gcggcgctga
     2461 tcagcgacga gcagctcgac cggatcgagg cgcgcgagaa ggtcgaggag gtcctgcact
     2521 tagagcggga gatgatgaac ggccgctcgc tgccgaacgc gatcgagctg gccggctggg
     2581 tcgaggaagg cgtccgccgc ctgctccgcc aggccgcgct cggtgagctc ggcaacgacc
     2641 tgatcggcac cgccgacgag accctgatcg ccaacggcct cgccgagggc gacgccgaga
     2701 tcacgatctc ggtcgccgaa gacttccccg agccggtgat cccggacgag ccgctgaacg
     2761 agagcgagcc ggtgatcgag cagctggcga tcccggagcc gcccgcggcg ttcgaagagc
     2821 ccgccgagtt ccggatcgaa cacgtgcccc ccgcacccga ggcaaccgag caggaggacg
     2881 acgaaggcat ggagcaagag acccgcatca tggagccgat cccggccgaa ggcgagatca
     2941 aggtcacggc catgccctgg ctcgacgagg tctccgtcgc ccgtcccggc gattcgctcg
     3001 aatggcccgc gccctcgaac gagatcgagc gcgacctcca gcaccgcgag cggatcgaca
     3061 ccccccgcgt gcgtcacctc ttcccggtcc cggactccga ctgggaggtc tcccacctgg
     3121 agttcgagta cagccgccgc ggcggcatgt cctagcgctg cgcgctacgg attagggacg
     3181 gtttcgccgt cccaggactt cgcccagatc gacaactatc tcggctgggt agttgtcgag
     3241 ctgggctccg gtgtagcggc ggacggcgaa gcccgcacgg cgcaggtgaa gctcgcgttt
     3301 gtggtcatct tcgaatgcct ggtgacctcg gtggtagtcg aagaagtcgg tttcgaccac
     3361 cactcgctcg gaacgccaga ggaaatcgac ttcgtagccg gcgacatcgg cgttcacctc
     3421 aggagccggg aacccgtgtt tggagaggaa cccgaggaag tcgatctcca ggtccgagcg
     3481 ggtgcgatcc gtagggagcg ccagctcgaa ccccatgaac tccgcctgct tctttgcccg
     3541 ccgcgccttc cagggctcga tcgtctgctc caagtccacg atcgtcctcc ggggcgtcgt
     3601 caccgggatc cgctcgcgcc gaattacttc cccttctctc ttcagagagg gagatcgatg
     3661 aagcacgatc ccacgctgag agaccttgcc actcatgctg ggcgaggtga cgtgcacggg
     3721 tgctccctcc ggtttcaaga acccccagag ataggccgcg ctctgatgcg aaagcgccgc
     3781 tccagtccca caggcgagca ccgccgccat gtagcgctgc cactcgttcg gggagcggtg
     3841 ccccaccgcg tagacgccac ggtggaggcg gtgcaggcgc ccgcttctct cccgttcgga
     3901 gatcgctgcc cgcgagatcc cgagggctga gagctgcgct gccgtcacca ccccgtgttg
     3961 tctccccgca gttctcgcga gcgctgcgtg ggggctatcg actttgttcg gcatacccga
     4021 acaaagtcga taggggtcga aattctcccc cggggtttgg gttaaaccag acccgcggcc
     4081 atcgctacgc gctcctcgcc ggttgcgaag aggtagcggt tgatttcggc gaggccggcg
     4141 aggtcgtgga cgtcctcgtc gaggtagggg acgcggatca cgcctttggt gcgcagctcg
     4201 ctggcgaggt gcttgatgtt gcgggcatcg cgctcggcca gggcctggta gtcggagaag
     4261 ttggcggcga cgcgctgggc gaggtcctcg tcgccgagct tctcggtcag ggacgtgggc
     4321 aggtcgtcga cctcgccgcg cagcttctcg gccgggtagt ggaccttgtt gacgatcacg
     4381 ccgccgaagg gcagcttcgc ctcgaccagt ttgcggtgga agtagacggc ctcgtcgatc
     4441 ggctcgccct gggggccgca gacgacgagg aagcaggttg agggatcggc gagcagtttg
     4501 ttgacccgtt tggcgcgcgc ctggaagccg tcgaccatgc cactgaaggc gttgaagaac
     4561 tcggccaggt cggcgagcag gtcgaagccg acgatccgtt tgaagatccc gagcgcgacc
     4621 gaggcgccgc ggccggcgac cttcgcggcg aggccggtcg gcttcatgaa catgcgcagc
     4681 gatttgcctt cgatgaactg ggtcagccgt ttcggcgcgt cgaggaagtc gagggcattg
     4741 cgggtgggag gggtgtcgag gacgagcagg tcgaagtcgc cttcggtgtg cagctcgaac
     4801 agcttctcca tcgccatgta ctcctgggag ccggcgaggg cgctggagat ctgctggtag
     4861 atccggtttt cgagcacccg gtcgcgcgac tcctcgtcgg gcgcctggcg cgcgatcagc
     4921 tcgtcgaagg tctgcttcgg gtcgagcatc atcgcccaca gctcgccctt catctcgacc
     4981 ccctgtttct cgaacagcgc ggggtcgacc cggcgtgcct cattgcccag ctccttcagc
     5041 ccgagcgagt cggcgagccg cttcgccggg tcaatcgtca gcacgcagac cttcaggccc
     5101 tgcgccgcca tcccggtcgc gatcgccgcc gaggtggtcg tcttgccgac cccgccggag
     5161 ccggcgcaga tgcagatctc cttgccctcg agcacctccc ccagcgtcgg catcagccca
     5221 gcctcttcga gagccgtcgc gccgcctcga ctccgagctc gggctcgaac aggaagggga
     5281 gggttttgac cggcgcctcg acccggcgcc gcagccgcgc cagctgggcc cgctgcgagc
     5341 gcgcccgccc gtgctcggag agcgccgccc gcgccgccgc ccgcgccgcg ccgtcctcgg
     5401 cctcagccac cttcgccagc ttctccgcct cggctttcga gaaccgctcc gggtagagcc
     5461 cgttgaggta gacgcggtcg accgcgaccc cgacctcgtc gcgcaggtcc ttctccagcg
     5521 ccgccgactc gttgaccggc atctcctccg gcagggcgac gattgccgtc ccggtgtgct
     5581 cgtggtcggt gatgaagcgg tcgagcgtct gcgcctgcga gtggatcggg ccgacccggg
     5641 cgatcgaggc gaaggtgcgc ggcgtctgca ggaagccgac cccgtggccg gtggccggtg
     5701 cgtcgacgat cacgaggtcg tacttgcgcc ccttcttgac cttgcggtcg agctgcgcca
     5761 gctcccagat tttcccgatc gtgaccagct ccttgaggcc cggcgtcgcc gcggcgaggt
     5821 agttgaagat gcggctgcgg aagagcatgt cgcgcatcgc ccgcaccttc agctgcagca
     5881 atacgtactc gcgcatcgac tcgtcggggt cgatcgagat cgaccagagg ttctcctcca
     5941 tctcgacctc gtggaagcca accgcggtgt gctcgaacat ccgcgaggtg ttctcctgcg
     6001 aggagacctc gcagacgatc gtgcgcttgc cttcggccgc cgcccgcagc cccagagcca
     6061 ccgcgacggt cgtcttgccc acgccaccct tgccggtaac gatcaccaac cgcttgtcga
     6121 ggagttcggg tatcgggcaa ttcctagcaa tttcgcgggc cggcaggaaa agccagcccc
     6181 tatccgaata tccgcgcaat gggatttcta agccgcaaga agaagaccgc tccggtttcc
     6241 gtcgaggagc agcacccgat tgtcgaggag ctgcaggaga tggaagaagt cgtgcccgag
     6301 tacgcgcatg aggagcccaa gtcggcgccc gtcgccgtcg aggacgacta ctccgacgag
     6361 tatgaggccg aggaggagga cgactacgcc gaggagccgg tcgaggccga gcctgccccg
     6421 gagcccgagc ctgtggctga gcccgctccc gtcgttcggg tggccgagcc ggtcgccgag
     6481 gtgcgcgagg agccggtcgc cgaagtggcc gaggaagagg aggacgacgg ccgcctcaag
     6541 gccgccgacc tgctcgcgcc gatgcgccag gacttcgtcg aggagcgccg cgccgccgag
     6601 caggcgccgg tgaagagcgc ggaagtgatc gccttcgcca accagaaggg cggcgtcgcc
     6661 aagaccacga ccacgctcaa cctcgccgtc gccttcgccg agtccggcta ccgcgtgctc
     6721 tgcatcgacc tcgaccctca gggcaacctg acgatgagcc aggggatcga cccggacaag
     6781 gtcgagaaaa gcctctacga cgtgctcgtc aacgacatgc cgatctcgga gatcatcatg
     6841 cgccgggaga tcgacatcgc cgtcgcctcg atcgacctgg ccggggcgga gatcgcgatg
     6901 agcaccaaga tcggtcgcga gcgctccctg gagaaggctc tgaaagaggt cgagggcgac
     6961 tacgacttcg tctgcatcga caccccgccg agcctcggcc tgctcacgat caacgcgctg
     7021 actgcggcaa acaaggtaat cgtccccgtg caatgcgagt atctctcgat gcgggggctg
     7081 gtacagctgc agaacacgct gaagatgatt caggagaatc tcaacccaga cgttcgcatc
     7141 gagggcatcc tgccgacgat gctggactcg cggaccgttc acgccaagga ggcggtggag
     7201 atcctcgagg agaacttcgg tgacctcgtt ttccagtccc ggatcaaaaa ggcgatcaaa
     7261 ttcgccgagg cgccggtgaa aggcgccagc gtgctcaagt acgattccgg cagcaacgcc
     7321 gcgaactact accgggagct ggctaaggag gtcctggcaa atggctcggc gtaacaagcg
     7381 ggcaagcatg cgcgagggcc ccctcgccga tctgttccgc tctaccgcag acgacgcacc
     7441 acaggatccg cccgaggcac cgcgctacgg ccgccaggat cccgccgagg ccaagaaagc
     7501 cgaggaagcc gccgcgaccg agcagcccgc cgccgcagcg cccgctccgc ctccggagcc
     7561 gccggcgccc gagcccaccc cggagcccga gccgcccgct cgggagcccg agcccgctcc
     7621 gccgcagccc gtcgccgacg cgccttacga ccacgcatcc gaggaaagct cggccgccag
     7681 cgcgcccgag cgcctcaaac gggttttcgc cgaggagccg cgtccgcgcg atcgcgagga
     7741 cttcctgccc ggctcgccgc gttacgggcg cgaggagccg gcctacgagc cgcccggcag
     7801 cggtgagccc caccagccgg tgatcaaagt cgtcggcgtc ggcggggccg gcgtcaacgc
     7861 ggtcaaccgg atggtcgacg cccgcatccc cggggtcgag ttcatggccg tcaacaccga
     7921 tcttcagtcg ctgcagctct gcgtcgccga cgtcaccgtg cacatcggcg aagagcagac
     7981 gcgcggcctc ggcgccggtg ccgaccccga ggtcggccac cgctccgcct tcgaggagca
     8041 ggacaagatc aagcgcttgc tcaagggctc cgacatggtc ttcgtcgccg ccggtgccgg
     8101 cggtggcacc ggttcgggcg ccgccccggt cgtcgcccga ctcgcccgcg aggtcggcgc
     8161 gctcaccgtc ggcatcgtga ccaagccgtt cagcttcgag ggcagccgcc gcggcaccca
     8221 ggccgagaag gggatcgagg aactggccgc cgaggtcgac accctgatcg tcgtccccaa
     8281 cgaccgcctg ctgcaggtgc tcgaccagca gacctcgatg gtcgaggcct tccaggtcgc
     8341 cgacgacgtc ctgcgccagg gcgtccaggg gatctccgac ctggtcaccc tgcccgccgt
     8401 gatcaacctc gacttcgccg acgtgcgcac gatcatgtcc gacgctggcc gcgccctgct
     8461 cgggatcggg atggcctacg gcgacgaccg cgcgatcctc gccgccgaaa aagcgatctc
     8521 ctcgccgctg ctggagacct cgatggaggg cgcccgctcg atcctgctct cgatcaccgg
     8581 cggctccgac ctctcgctgg tcgaggtctc cgaggccgcc aaggtggtcg gcgaggccgc
     8641 ccacccggag gcgaacatca tcttcggcgc caacgtcgac gaggagctga ccgaccagat
     8701 ctgggtcacc gtcgtcgcca cccgcttcga cggccgcccg agcacgccgc gccggtcctc
     8761 cgagccgacg atccgtcgca ccaaagacgc gccggccgag cgccgctccg gccgcggtga
     8821 gcgcggcggc gacctcggca tcgacgtccc cgagtttctg ccgagctagc gagctgaccc
     8881 catgggaggg gtcgttgcgg ccggtcaccc gctcagcgcc gaggccggcg ccgaggtgct
     8941 gcgcgagggc ggcaacgcgg tcgacgcggc gatcggcgcc gtgctcgcct cgttcgcggt
     9001 cgagagcccg ctgaccgggt tcggcgccgg cggctacatg ctcgtccacc gggaggcacc
     9061 tggaggtgtc cccggctccg gtcgcaaaga gtcagatagc gatgctccct ccgccggggt
     9121 caccgaacag accttgatcg atttctttgt ggccgcgccc ggcctcgacg ggatcgagcg
     9181 gcgcgccgag ctggtgccgg tcccagtcca cttcgacgcc gagacctcgc agacgttctt
     9241 cgtcggcccg gcctcctgcg gc
//
LOCUS       DASRIG010000316         8249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2228957, whole genome shotgun sequence.
ACCESSION   DASRIG010000316 DASRIG010000000
VERSION     DASRIG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228957"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VFB52_08580"
     CDS             <1..1604
                     /locus_tag="VFB52_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZO06429.1"
                     /translation="KVPVVPNIGTIGLGIAKAGADIITLSGFEGGTGAARQHALRHVG
                     LPSDIGTRAVHRALMEAGLRNRVEIWADGGYRTGHDVVKLHCLGANRVGFGTLAMVSL
                     GCTICRGCQLDTCHVGIATQIETTEEAQEHGLKKFTPQEVDRAAESCARFFRSMGEEV
                     KQVVASLGYERAQDLVGRYDLLEQIGMKDKIDLAPLITPLEEFLDLEPLDLPVEEKEL
                     VEARAEAGLVVARPIRMEAKQASSQIAALAPEVCAGKTLRSEFPRATDANDRVLGTEL
                     AGAIARSRIFEDGPEPSEEVLAELNFNGGSVAGQGLGAFNAYGVSIRVEGGAQDGVGK
                     AMLGGTIAILKGKGARGKRLNGSVGKSFAYGAQRGQLYVQGSADSRFCIRLSGADVIL
                     GGEPEQDIDDSRGCIVDRANAKGFAFEYMTSGRAVVLGDLGPWACAGMTGGRVYVRHN
                     AFGIDRDAIQRRLGEGAKVELKDLDSEGLLDLDDLLNGYAKELRATGQDEEADRILDL
                     SADATSNFLMVIPKKVQADPSISTE"
     gene            complement(1678..2163)
                     /locus_tag="VFB52_08585"
     CDS             complement(1678..2163)
                     /locus_tag="VFB52_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06430.1"
                     /translation="MNAAPSPVDPPTGPQGPRGPQEVPPSISAAADRARAELHAEIER
                     VRLGVEEVLAEQGGPPDEELQRELANLREDTRLYVKKRLRKTEKKLHRSVEKVEDSVE
                     RLDERTQRLEARIDEVEADRAAAEVRIHSDTERMLDGLLQEVRSIADMLTKDSPQPPS
                     A"
     gene            complement(2203..2835)
                     /locus_tag="VFB52_08590"
     CDS             complement(2203..2835)
                     /locus_tag="VFB52_08590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZO06431.1"
                     /translation="MTGDQSGTRIGEDSGRRVTIFDSVSASPLDDPYAAGFIPGCGTY
                     FCLSCGSQLALRETDELPQCSDCGGSRFRRDSIFSAHQDHEATTTVEHALPTEHELPE
                     WLDAARALLTGRGEHLVYRDDEGGIETFTIERGWTRIGRSEAADVCLDDPTVSRRHAL
                     IVAEPGKGLKVLDDRSLNGVFVNGEPAELKTLRHGDEITVGRFRLYLLRT"
     gene            2890..3405
                     /locus_tag="VFB52_08595"
     CDS             2890..3405
                     /locus_tag="VFB52_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO06432.1"
                     /translation="MGPVAAEIEIDVPREEAFELLIDLSARPSFTDHFLSDFHLTRID
                     SRGIGAGARFRVNAPLRSPWEDTTIVEVEAPHKIVEEGFGGRQNRIPNRTVWELTSGS
                     GGLTTVRVAHWTESRFIDRGLEVLSGGSFWQERGWKRALHRLRDNLEGENAAVERISV
                     AGGNRYATGIP"
     gene            3458..3991
                     /locus_tag="VFB52_08600"
     CDS             3458..3991
                     /locus_tag="VFB52_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06433.1"
                     /translation="MLSLTAALALGALAVGVSACGYESHELEVSEGHVFELGELEYVV
                     TFSRYLNPDDNEDASYLVGQENPPPNGETYFGIFFEVQNDTDESHTLPEEMWIIDADK
                     QRYDVVPSESLYAFPFGGEVEEHEQIPVLDSTPDLGPIEGSLVVFLLPEEANENRPLI
                     LHVPGPDGEEAEVTLDL"
     gene            complement(4017..5681)
                     /locus_tag="VFB52_08605"
     CDS             complement(4017..5681)
                     /locus_tag="VFB52_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZO06434.1"
                     /translation="MAFVAFIIGAALGAPGSPEKEAANRFGEAWSARDYKAMYQELND
                     DSRAQFSLAEFSDAYQSAAETSTLRGIEPGSPHDPQKIDGTTAVPLPLTLTMVAFGRV
                     EDELLLPWESGGIAWNPSLIFPGLEEGEGLSSEIELAPRAPILAADGTALAEGPAEER
                     EHPLGSAAVDVTGEVGEPSEEDVARLAKHGFTPETPVGITGLERAFNIRLAGKPGGKL
                     LAVDESGGSARVLAESEPEEGAPVKTTIDPELQEAAVASLAGRFGGVAVLDSRNGDVR
                     AVAGQAFSAPQPPGSTFKIVTTTAALQKNVVQLDDEFEYNEGINVGGRFLYNANGEIC
                     GGTFVQAFAHSCNAVFAPLGPKVGNDALVSTAERFGFNEQPSLYAPKYLAEAGLPEST
                     IPTEIPTEIELGVSAIGQGEVQATALEMASVAQTVANGGVRMPTSIVNNKKLRPDAEP
                     VRVMSKKIADELTELMVGVVVEGTGTAGAISEAQVAGKTGTAELGPKEGQENEENPIQ
                     IKDAWFAAFAPADKPRLAVGVMLIEAEASGGEVAAPIAAEVLSAGL"
     gene            5797..6939
                     /locus_tag="VFB52_08610"
     CDS             5797..6939
                     /locus_tag="VFB52_08610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HZO06435.1"
                     /translation="MTVLDWAIVAFTIALALWGYRQGLIVGALTLAGFGVGAFAGSRL
                     APLVLSDGAESPYAPLCAALGALLVGALAAVAVESFALGLRDKLIRHHTLHLADGAGG
                     AALIAAVALGLVWVFGAVALHAPATERLRGDVQDSVILRSLNEVLPPSGPVLNALDRV
                     DPAPAVSGPATPTEPPDPAIAHDPDVLAAGESVVRVLSTACGLGIEGSGWSVAPGLVV
                     TNAHVIAGSDDTTVTTQDGVELDATAVYYSPANDLALLRVGAEIPVLEISLRDEEGQR
                     GAVLGYPENGPYSLEAARIGETKETISEDSYGRGPIERDIIAVGGRVRSGNSGGPVVD
                     AQGRVVGTIFAATTSGLPGGFAIPAGLVRKAVTQASEWTSTGPCTL"
     gene            7052..7354
                     /locus_tag="VFB52_08615"
     CDS             7052..7354
                     /locus_tag="VFB52_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06436.1"
                     /translation="MCPDFHAAIELIGKRWTGAIVSALTERPMRFGELRKAVPGLSDR
                     LLSQRLRELEEEGLVEREVEAGTPVRVTYSLTEAGEGLSPALSELKQWAKRWRCTG"
     gene            7399..>8249
                     /gene="xseA"
                     /locus_tag="VFB52_08620"
     CDS             7399..>8249
                     /gene="xseA"
                     /locus_tag="VFB52_08620"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZO06437.1"
                     /translation="MDLPGPFPVGRYARKLQEEMRKRARVQLMGEVTGVGRSKVQVFF
                     ELRDAEGAVPCSMWLNDLEKADLPEGAMRDGAELVIGGGPDYYPGGGQASPSFSFRAT
                     QVRLAGEGDLLARLAALRKQLQAEGLFELQKQLRRPLLPKTIGVVTAESGAARRDLLA
                     GLERRGWEGTVVWAFAPVQDRRAAPAITAAIQDLAARPEIEAIVVTRGGGSLADLWAF
                     CDETLCRTVAMLRVPVISAVGHERDSTLIDDVAAVSCSTPTHAAEAAVPLDCLAARGE
                     LRRGAGR"
BASE COUNT         1180 a         2822 c         2939 g         1308 t
ORIGIN      
        1 tgaaggtccc ggtggtcccg aatatcggga cgatcgggct cgggatcgca aaggcgggcg
       61 ccgacatcat caccctgtcg ggcttcgagg gcggaaccgg cgccgcgcgc cagcacgcgc
      121 tgcgccacgt cggcctgccg agcgacatcg gcacccgcgc cgtccaccgg gcgctgatgg
      181 aggcggggct gcgcaaccgg gtcgagatct gggccgacgg cggctaccgg accggccacg
      241 acgtcgtcaa gctgcactgc ctgggggcga accgggtcgg cttcggcacc ctggcgatgg
      301 tcagcctcgg ctgcacgatc tgccgcggct gccagctcga cacctgtcac gtcgggatcg
      361 cgacccagat cgagaccacc gaggaagcgc aggagcacgg cctcaagaag ttcaccccgc
      421 aggaggtcga ccgtgcggcc gagagctgtg cccgcttctt ccgctcgatg ggcgaggagg
      481 tcaagcaggt ggtcgcctcg ctcggctacg aacgcgccca ggacctggtc ggccgctacg
      541 acctgctcga acagatcggg atgaaggaca agatcgacct ggcgccgctg atcacgccgc
      601 tggaggagtt cctcgacctc gagccgctcg acctgccggt cgaggaaaaa gagctggtcg
      661 aggcgcgggc cgaagccggc ctcgtcgtcg cccggccgat ccggatggag gccaagcagg
      721 cctcctcgca gatcgctgcg ctggcgccgg aagtctgcgc cggaaagacc ttgcgcagcg
      781 agttcccgcg ggcgactgac gccaacgacc gggtgctcgg gaccgagctg gcgggcgcga
      841 tcgcccgctc gcggatcttc gaggacgggc cggagccgag cgaggaggtg ctcgccgaac
      901 tcaacttcaa cggcggctcc gtggccggcc agggcctcgg cgccttcaac gcctacgggg
      961 tctcgatccg ggtcgagggc ggtgcccagg acggcgtcgg caaggcgatg ctgggcggga
     1021 cgatcgcgat cctcaagggc aagggcgcgc gcggcaagcg gctgaacggc tcggtcggca
     1081 agtcctttgc ctacggcgcc cagcgcggcc agctctacgt ccagggctcg gccgactcgc
     1141 gcttctgcat ccgcctctcc ggcgccgacg tgatcctcgg cggcgaaccc gaacaggaca
     1201 tcgacgacag ccgcggctgc atcgtcgacc gcgccaacgc caagggcttc gccttcgagt
     1261 acatgaccag cggccgcgcc gtcgtgctcg gcgacctcgg tccctgggcc tgcgccggga
     1321 tgaccggcgg ccgcgtctac gtccgccaca acgccttcgg catcgaccgc gacgcgatcc
     1381 agcgccgcct cggcgagggc gccaaagtcg aactcaagga cctcgactcc gagggcctac
     1441 tcgacctcga cgatctgctc aacggctacg ccaaggagct gcgcgccacc ggccaggacg
     1501 aggaggccga ccgcatcctc gacctctccg ccgacgccac cagcaacttc ctgatggtga
     1561 tcccgaagaa ggtccaggcc gacccaagta tttcaaccga gtgatgatgt gttaggcggg
     1621 aggagtcggg gggctccttc cgcttaactt tttcttcggc gccgcgcgcc ggccagctca
     1681 ggcgctcggt ggctggggcg agtccttggt cagcatgtcg gcgatcgagc ggacctcttg
     1741 caggaggccg tcgagcatgc gctcggtgtc ggagtggatg cggacctcgg cggcggcgcg
     1801 gtcggcctcg acttcgtcga tccgggcttc gaggcgctgg gtgcgctcat cgaggcgctc
     1861 gaccgagtcc tcgaccttct cgaccgagcg gtggagcttc ttctcggttt tgcgcaggcg
     1921 cttcttgacg tagaggcggg tgtcctcgcg caggttcgcc agttcgcgct ggagttcctc
     1981 gtcgggcggg ccgccctgtt cggccaggac ctcttcgact ccgaggcgga cgcgctcgat
     2041 ctcggcgtgc agctcggcac gggcgcggtc ggcggcggcg ctgatcgagg gcggaacctc
     2101 ctggggcccg cgcggtcctt gtgggcccgt gggggggtct accgggctcg gggcggcatt
     2161 cacgggcccg atcctaggga cggggctgga cgcgcttgga gcctaggtgc ggagcaagta
     2221 gagccggaac cggccaaccg tgatctcgtc gccgtggcgc agcgtcttca gctcggccgg
     2281 ctcgccgttc acgaacacgc cattcaggct gcggtcgtcg agcaccttca gacctttgcc
     2341 cggttcggcg acgatcagcg cgtggcggcg ggagacggtc gggtcgtcga ggcagacgtc
     2401 ggcggcctcg ctgcggccga tccgggtcca accgcgctcg atcgtgaagg tctcgatccc
     2461 gccctcgtcg tcgcggtaga cgaggtgctc gccgcgcccg gtcagcagcg ctcgggcggc
     2521 gtccagccac tcgggcagct cgtgctcggt gggcagggcg tgctcgacgg tcgtggtcgc
     2581 ttcgtggtct tggtgggcgc tgaagatcga gtcgcgcctg aaccgcgagc cgccgcagtc
     2641 agagcactgc ggcagctcgt cggtctcgcg tagggccaac tgcgagccac atgaaaggca
     2701 gaaataggtg ccgcaaccgg ggatgaagcc ggctgcatag ggatcgtcca gcgggctggc
     2761 gctaacgctg tcgaagatgg tgaccctcct tcccgagtcc tctccgatcc tagtacccga
     2821 ctggtctccc gtcactcaaa catcctcgtc cggtggcgcc gcggggcggg tgaatagaat
     2881 cgcgcggcga tgggccccgt agccgcagag atcgagatcg acgtcccccg cgaggaggcg
     2941 ttcgagctgc tgatcgacct ctccgcccgg ccctcgttca ccgaccactt cctctccgac
     3001 ttccacctca cccggatcga ttcgcgcggg atcggcgccg gagcccgttt ccgggtcaac
     3061 gcgccgctgc gctcgccgtg ggaggacacg acgatcgtcg aggtcgaggc gccgcacaag
     3121 atcgtcgagg agggtttcgg gggccgccag aaccggatcc cgaaccgaac cgtctgggag
     3181 ctgaccagcg gctccggcgg gctgaccacg gtgcgcgtcg cccactggac cgagtcccgc
     3241 tttatcgacc gcgggctcga ggtcctctcc ggtggatcgt tctggcaaga gcgcggctgg
     3301 aagcgggcgc tgcaccggct gcgcgacaac ctcgagggcg aaaacgccgc tgtagagcgg
     3361 atttcggtcg ccggtggcaa tcgctacgcg accggcatcc cttgatctgc ccctcttaat
     3421 agactcccgc ccgctgatgt cgttgcctcg cagactgttg ctgtcgctca ccgccgccct
     3481 ggccctcggg gcgctcgccg ttggcgtctc cgcctgcggc tacgagagcc acgagctgga
     3541 ggtctccgag ggtcacgtgt tcgagctcgg cgagctggaa tacgtcgtca ccttctcgcg
     3601 ctacctcaac cccgacgaca acgaggacgc gtcctacctg gtcggccagg agaacccgcc
     3661 gccgaacggc gaaacctact tcgggatctt cttcgaagtc cagaacgaca ccgacgaatc
     3721 gcacacgctc cccgaggaga tgtggatcat cgatgccgac aaacagcgct acgacgtggt
     3781 gccgagcgaa agcctctatg ccttcccctt cggcggcgaa gtcgaagaac acgaacagat
     3841 cccggtgctc gactcgaccc cggacctggg tccgatcgag ggctcgctgg tggtcttcct
     3901 gctgcccgaa gaggcaaacg agaaccggcc gctgatcctc cacgtccccg gtcctgacgg
     3961 cgaagaggcc gaggtcaccc tcgacctcta ggccttaggg gccttgcggc tgggcctcag
     4021 agcccggccg agagcacttc cgccgcgatc ggcgcggcga cttccccgcc cgaggcttcc
     4081 gcttcgatca gcatcacccc gaccgcgagg cgcggcttgt cggcgggggc gaaggcggcg
     4141 aaccaggcgt ccttgatctg gatcgggttc tcctcgttct cctgaccctc tttcgggccg
     4201 agctcggcgg tgccggtctt gccggcgacc tgggcctcgc tgatggcgcc cgcggtcccg
     4261 gtcccttcga ccacgacgcc gaccatcagt tcggtcagct cgtcggcgat cttcttcgac
     4321 atcacccgca ccggctcggc gtcggggcgc agttttttgt tgttgacgat cgaggttggc
     4381 atccgcacgc cgccgttggc gacggtctgg gcgacgctcg ccatctccag cgccgtcgcc
     4441 tgcacttcgc cctggccgat cgccgagacc ccgagttcga tttcggtcgg gatttcggtc
     4501 gggatcgtcg attcgggcag gccggcttcg gccaggtact tgggcgcgta gaggctcggt
     4561 tgttcgttga acccgaagcg ttcggcggtc gagaccaggg cgtcgttgcc gactttcggc
     4621 ccgagcgggg caaagaccgc gttacacgag tgggcaaagg cctggacgaa ggtgccgccg
     4681 cagatctcgc cgttggcgtt gtagaggaag cggccaccga cgttgatccc ttcgttgtat
     4741 tcgaattcgt cgtcgagctg gacgacgttc ttctgcagcg ccgccgtcgt ggtcacgatc
     4801 ttgaaagtcg agcccggggg ctggggggcg gagaaggcct ggccggcgac cgcgcgcacg
     4861 tcgccgttgc gggagtcgag cacggcgacc ccgccgaagc gtccggccag cgaggcgacc
     4921 gcggcttcct gcagttcggg gtcgatcgtc gtcttcaccg gcgccccttc ttcgggttcg
     4981 gattcggcca gcacccgcgc tgagccgccg ctttcgtcga ccgccagcag tttgccgccg
     5041 ggcttgccgg cgaggcggat gttgaaggcg cgctcgaggc cggtgatccc gaccggcgtt
     5101 tccggcgtga acccgtgctt ggcgagccgt gcgacatcct cctcggaggg ttcgccgact
     5161 tcgccggtga cgtcgaccgc ggcgctgccc agcgggtgtt cgcgttcctc ggccgggccc
     5221 tcggccagcg cggtgccgtc ggcggcgagg atcggcgctc gcggcgccag ctcgatttcg
     5281 ctgctcaacc cttcgccttc ttcgagcccg gggaagatga gactcgggtt ccaggcgatc
     5341 ccgccacttt cccagggaag cagcaactcg tcttcgaccc ggccgaaggc gaccatcgtc
     5401 agcgtcagcg gcagcggcac cgcggtcgtg ccatcgatct tctgggggtc gtgcggggag
     5461 cccggttcga tcccgcgcag ggtcgaggtc tcggccgcgc tctggtaggc gtcgctgaac
     5521 tcggcgaggc tgaactgggc ccgcgagtcg tcgttcagct cctggtacat cgctttgtag
     5581 tcgcgcgccg accaggcctc gccgaagcgg ttcgcggcct ccttctcggg cgatccgggc
     5641 gcgccgaggg cggccccgat gatgaacgcg acgaatgcca ccagggcgag ggggagcgtg
     5701 cgggtaaaga gacggcgccg acgctccgcc tcgggtgaca tacgactcac cggcagaaag
     5761 gtaatagagc ggtctcccgc gtgcttctat tcatatgtga ctgttctcga ttgggcgatc
     5821 gtcgccttca ccatcgcgct cgccctttgg ggctacaggc agggattgat cgtcggcgcg
     5881 ctcactttgg cgggcttcgg ggtcggcgcg tttgccggca gccggctggc gccgctggtc
     5941 ctctccgacg gcgccgagtc gccctacgcg ccgctctgcg ccgctctggg agcgcttttg
     6001 gtcggcgcgc tggcagcggt cgcggtggag agcttcgcgc tcggcctgcg cgacaagctg
     6061 atccgccacc acaccctcca cctcgccgac ggggcgggtg gggcggcgtt gatcgccgcg
     6121 gtcgcgctcg ggctggtctg ggttttcggc gccgtcgccc tgcacgcgcc ggcgaccgag
     6181 cggctgcgcg gcgacgtcca ggactcggtg atcctgcgca gcctgaacga agtcttgccg
     6241 ccttcggggc ccgttctcaa cgcccttgat cgcgtcgatc cggcgccggc ggtctccggt
     6301 ccggcaaccc cgaccgagcc gcccgacccc gcgatcgccc acgaccccga cgtgctcgcc
     6361 gccggcgaat cggtcgtgcg cgtgctcagc accgcctgcg ggctcgggat cgagggctcg
     6421 ggctggtcgg ttgcgcccgg gctggtcgtc accaacgcgc acgtgatcgc cggctccgac
     6481 gacaccacgg ttacgaccca ggacggggtc gagctcgacg cgacggcggt ctactactcg
     6541 ccggccaacg acctggcgtt gctgcgggtc ggggccgaga tcccggtgct ggaaatctcc
     6601 ctccgcgacg aggaggggca gcgcggggcg gtgctcggct acccggagaa cggtccctac
     6661 tcgctggagg ccgcccggat cggcgagacc aaggagacga tcagcgagga ctcctacggt
     6721 cgcggcccga tcgagcgcga catcatcgcg gtcggcgggc gggtgcgcag cggcaactcg
     6781 ggcgggccgg tggtcgacgc ccaaggccgc gtggtcggca cgatcttcgc cgccaccacc
     6841 agcggcctgc cgggcgggtt cgcgatcccc gccgggttgg tccgcaaagc ggtcacgcag
     6901 gccagcgagt ggaccagcac cgggccgtgc acgctgtgaa agtgggaaag cccgcaactt
     6961 acgttcagta agctgggaag cgatggaacc ggtcgcaacg accccggccc aggcgggccc
     7021 ggccccggtc ccaagcgggc aggctgactg tgtgtgtccc gacttccacg cggcgatcga
     7081 gctgatcggc aagcgttgga ccggggcgat cgtcagcgcg ctgaccgagc ggccgatgcg
     7141 cttcggcgag ctgcgcaaag ccgtgcccgg cctctccgac cgcctgctct cgcagcgcct
     7201 gcgcgagctc gaggaggagg ggctggtcga acgcgaggtc gaagcgggga cgccggtacg
     7261 ggtcacctac tcgctgaccg aggcgggaga aggtctcagc ccggccctca gcgagttgaa
     7321 gcagtgggcc aagcgctggc gctgtacggg ctgagacgct tcgtcaggcc cgtcagggcc
     7381 gtggaatagc ctcaaacaat ggatctcccc ggaccgtttc ccgttgggcg gtatgcccgc
     7441 aaactccagg aggagatgcg caagcgggcg cgggtgcagc tgatggggga ggtgacgggc
     7501 gtcgggcgca gcaaggtgca ggtcttcttc gagctgcgcg acgccgaggg cgcggtgccc
     7561 tgctcgatgt ggctgaacga cctggagaag gcggatctgc ccgagggggc gatgcgcgac
     7621 ggcgctgaac tggtgatcgg cggcgggccc gactactacc cgggcggcgg ccaggcttcg
     7681 ccgagcttca gcttccgcgc gacccaggtg cggctggccg gtgagggcga tctgctggca
     7741 cggctggcgg cgctgcgcaa acagctgcag gcggaggggc tgttcgagct gcagaagcag
     7801 ttgcgccggc cgctcctgcc gaagacgatc ggggtggtga cggcggagag cggcgcggcg
     7861 cggcgcgacc tcctcgccgg gctcgagcgg cgcggctggg aggggacggt ggtctgggcc
     7921 ttcgcgccgg tccaggaccg gcgcgcggcg ccggcgatca ccgccgcgat ccaggacctg
     7981 gccgcgcgcc cggagatcga agcgatcgtc gtcacccgcg gcggcggcag cctcgccgac
     8041 ctctgggcct tctgcgacga gacgctctgc cggaccgtgg cgatgctgcg cgtgccggtt
     8101 atcagcgccg tcggccacga gcgcgactcg accttgatcg acgacgtcgc cgcggtctcc
     8161 tgctcgaccc cgacccacgc ggccgaggcg gccgtgccgc tcgactgcct cgccgcccgc
     8221 ggcgagctgc ggcggggcgc cggacgcat
//
LOCUS       DASRIG010000317         6139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2228974, whole genome shotgun sequence.
ACCESSION   DASRIG010000317 DASRIG010000000
VERSION     DASRIG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228974"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..903
                     /gene="whiG"
                     /locus_tag="VFB52_08625"
     CDS             130..903
                     /gene="whiG"
                     /locus_tag="VFB52_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HZO06438.1"
                     /translation="METGVKEVELRDLWRRYKDDGDDGARERLVVAYSPMVKFVAGRL
                     GAGLPSHVEDADLISYGLVGLIGAIERFDPDRGIKFETFAMTRIRGSIIDELRSLDWV
                     PRSVRSRAREIEACQAKLEHELQRAPSEAELAAKLKMTEEELQTALLEIANSSVYALD
                     ELWTVSDSSGDQVSLLDTIADDGAADPQEALASTEVKDRLTEAISSLPEREQLVVALY
                     YYENLTLREIGEVLGVTESRVSQLHTKAVMRLKSHLQQA"
     gene            905..1288
                     /locus_tag="VFB52_08630"
     CDS             905..1288
                     /locus_tag="VFB52_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06439.1"
                     /translation="MRLRLQRRQRRYDTHGLTPFERAVLRFNTSDARHTVEGLSRTLG
                     EATVSVGAAAGAADEIRITVAWELTWYQWGVDLGDESREVYERAQGKLIGQLDAAARQ
                     WNASLVEGGRIVLVAPRRRPGGRRR"
     gene            1288..1689
                     /locus_tag="VFB52_08635"
     CDS             1288..1689
                     /locus_tag="VFB52_08635"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HZO06440.1"
                     /translation="MAPFTVNVDGGARGNPGPAAIGVVLRDPDGAVVEELGETIGKAT
                     NNVAEYRALLRGIELAAAHGATELELIGDSELVVRQIEGRYKVKDATLRELYEEAKRE
                     LTGFESWSIRHVRREANADADRLVNTALDGG"
     gene            1689..2177
                     /locus_tag="VFB52_08640"
     CDS             1689..2177
                     /locus_tag="VFB52_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06441.1"
                     /translation="MSEPIDPGVDIGHVHLKVADVDRALDFYCGVLGFELMQRYGPDA
                     AFISAGGYHHHIGLNSWESKGGPPPARGTTGLFHTAIRYPTRRSLADALQRLIDARIP
                     LTGASDHGVSEALYLDDPDGNGVELYWDRPKAEWPEPQPGEKVQMFTRPLDLEGLMAE
                     LS"
     gene            complement(2174..3076)
                     /locus_tag="VFB52_08645"
     CDS             complement(2174..3076)
                     /locus_tag="VFB52_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZO06442.1"
                     /translation="MGSIDPGKPPGGGHLNQEDSMRRGTYGLVLGLIATVMAVAAVLI
                     LPVAASAWAPAKTAAIHPGVQTRTEGGQCTANFIFKEGDTVLIGQAAHCSGTGGQTET
                     DGCTSGSLPLGTPVEVTGAKQPGTLVYNSWLAMQAAGETNPDACAYNDLALVRLAASD
                     VANVNPSVPGFGGPTGVGTVGDLGDTVYSYGNSELRGGITKLSPKLGIVIQNEGNGWS
                     HNVVTLTPGIPGDSGSGFLNGEGEAFGVLSTLQLAPLPATNGVGDLAKELAYARAHSQ
                     FGAVQLVPGTEPFKGNLLGAILGS"
     gene            3135..4484
                     /locus_tag="VFB52_08650"
     CDS             3135..4484
                     /locus_tag="VFB52_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06443.1"
                     /translation="MAVFTTDVTRPVYVFRRLVALVAALVAVSAPAAAVAAPGDVTTY
                     PLAPGADPRGIVSGPGGALWFTQGGAGGLGRITTAGEIAGFPAAPTGSALQLGPIAVG
                     PDGNLWYGGLGGLGRMTPAGVAESFPFSDFPKQLAAGPDGALWFSGVENGRLGRITTA
                     GELTYVELPNSIGPAPADQTEVTGITTGPDGNVWFGSVNGRCYCGKGLIPGEGRIGTI
                     SAGGVPSEIAIPGGGQPLSIAPGPDGAIWFTFGLGAVGRYAGGRFSIVVTRSTIAFRS
                     LVAGADGNLWFAVGRGIGRLTPSGHLTVFRLAERARWLTSGSDGNLWFTTAGAVGRLE
                     PGKPGVELIPPKRRPRNGRLAVDLFCSGGGAAACAGRAKLIKKTFLRREHGEATYRTR
                     LLGRGRYSVPGESGGRMVVRLNSLGRKLVREGPLSATLVANAGAGVGARSQVRLRRP"
     gene            4492..5133
                     /locus_tag="VFB52_08655"
     CDS             4492..5133
                     /locus_tag="VFB52_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06444.1"
                     /translation="MEVAIAGGHGKIAMLLGKALAERGDIVWGLIRNPDHKDDLQAAG
                     IEPVLCDLEAGDDVAAGVRGAEAVVFAAGAGPGSGAGRKQTMDYEGAVKLIEAAKAEG
                     IRRYLMVSSIGAGRAPAEGGDVFGEYLRAKAAADQALAESGLEYTIVRPGPLTDDPPR
                     GLVQVGVDLDRVEIPRADVAAVLAACLDSRHTIDRTFEVVGGDIAIDAALASI"
     gene            complement(5134..>6139)
                     /locus_tag="VFB52_08660"
     CDS             complement(5134..>6139)
                     /locus_tag="VFB52_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZO06445.1"
                     /translation="TPWALLSGDSLFIGDVARPDLAIEPRDGAAQMFHTLHERLLTLD
                     DEVEVWPGHLGGSLCGGSGLDLKNSSTIGFERRHNKALAILDEAEFVEDAVGSIGDKP
                     PNVAEIVALNRGPLVDDFGTPAPMSPHEVEECVAAGAVVIDGRTNEQFDEAHIAGALS
                     ASSYDTGFATKVTRVAPPGADVIVVAASSGDERAAAELLAAVGMRVRGFLEGGMTAWR
                     VEERPVQRIELIDPEELASRVEGPQPPLILDVRNAAEYAGEHIPGSLHIPYGDLAGRF
                     DEIPRERQIAAICRGGKRSGLAASILQREGFDVVHVGRGVGAWRDGGHPVQSGAAATV
                     "
BASE COUNT          889 a         2169 c         2182 g          899 t
ORIGIN      
        1 catgcgtcga tctcgactac ccaggtctac accagggtag atgctgcacg tttacgtgac
       61 acctacgctg caagccaccc gcgagcatga gttgattcgt ctattcggcc ataaaatccg
      121 cgcactctga tggagacggg cgtaaaagag gtagagctcc gcgatctctg gcgtcgctac
      181 aaagacgacg gggacgacgg tgcccgcgag cggctggtcg tcgcctactc gccgatggtc
      241 aagttcgtcg ccggccggct cggcgccggg ctgccctcgc acgtcgagga cgccgacctg
      301 atctcctacg gcctggtcgg gctgatcggg gcgatcgagc gcttcgaccc cgatcgcggg
      361 atcaagttcg agaccttcgc gatgacccgg atccgcggct cgatcatcga cgaactgcgc
      421 tcgctcgact gggtgccgcg ctcggtgcgc tcgcgcgccc gtgagatcga agcttgccag
      481 gcgaaactcg agcacgaact gcagcgggcg ccgagcgagg ccgagctggc cgcgaaactg
      541 aagatgaccg aggaggaact gcagaccgcg ctgctggaga tcgccaactc ctccgtctac
      601 gcgctcgacg agctctggac cgtctccgac tcctctggcg accaggtcag cctgctcgac
      661 acgatcgccg acgacggcgc cgccgacccg caggaggcgc tcgcctcgac cgaggtcaag
      721 gaccgcctga ccgaggcgat cagctcgctg cccgagcgtg agcagctcgt cgtcgccctc
      781 tactactacg agaacctcac cctgcgcgag atcggcgagg tcctcggggt gaccgagtcg
      841 cgcgtctcgc agctgcacac caaggccgtg atgcggctga agtcccacct ccagcaggcc
      901 tgacatgcgc ctgcgcctgc aacggcggca gcgacgctac gacacccacg ggctgacccc
      961 gttcgagcgc gccgtcctcc gcttcaacac cagcgacgcc cgccacacgg tcgagggact
     1021 gagtcgcacg ctcggcgagg cgacggtctc ggtcggggcc gccgccggcg ccgccgacga
     1081 gatccggatc acggttgcct gggagctgac ctggtaccag tggggcgtcg acctcggcga
     1141 cgagtcgcgc gaggtctacg agcgcgccca gggcaagctg atcggccagc tcgacgccgc
     1201 cgcgcgccag tggaacgcct cgctggtcga gggcggccgg atcgttctgg tcgcgccgcg
     1261 gcgtcggccc ggcgggcggc gccgctgatg gcgccgttca ccgtcaacgt cgacggcggc
     1321 gcccgcggca acccgggccc ggcggcgatc ggcgtcgtcc tccgcgaccc cgacggggcg
     1381 gtggtcgagg agctggggga gacgatcggc aaggcgacca acaacgtcgc cgagtaccgg
     1441 gcgctgctgc gcgggatcga gcttgcggcc gcccacgggg cgaccgagct ggagctgatc
     1501 ggcgactcgg agctggtcgt gcgccagatc gaaggccgct acaaagtcaa ggacgcgacc
     1561 ctgcgcgagc tctacgaaga ggccaagcgc gagctgaccg ggttcgagag ctggtcgatc
     1621 cgccacgtcc gccgcgaggc caacgccgac gccgaccggc tcgtcaacac ggccctggac
     1681 gggggctgat gagcgagccg atcgaccccg gcgtcgacat cggccacgtc cacctgaagg
     1741 tcgccgacgt cgatcgcgcg ctcgacttct actgcggcgt gctcggcttc gagctgatgc
     1801 agcgctacgg ccccgacgcg gccttcatct ccgccggtgg ctaccaccac cacatcgggc
     1861 tgaactcgtg ggaatcgaaa ggcggcccgc cgcccgcgcg cgggaccacc gggctcttcc
     1921 acacggcgat ccgctacccc acccgccgct ccctcgccga tgccctgcag cgcctgatcg
     1981 acgcccgcat cccgctcacc ggcgcctccg accacggggt cagcgaggcg ctctaccttg
     2041 atgaccccga cggcaacggg gtcgagctct actgggaccg accgaaagcg gagtggccgg
     2101 agccgcagcc aggcgagaag gtccagatgt tcacccggcc actcgacctc gaagggctga
     2161 tggcggaact ctcctaggag ccgaggattg ctcctaggag attgcctttg aacggctcgg
     2221 tgccggggac gagctggacg gcgccgaact ggctgtgggc gcgggcgtag gcgagctctt
     2281 tggcgaggtc gccgaccccg ttggtcgccg gcagcggggc cagctgcagg gtgctgagca
     2341 cgccgaaggc ctcgccctcg ccgtttagga agccgctgcc ggagtcgccg gggatccccg
     2401 gggtgagggt gaccacgttg tggctccagc cgttgccttc gttctggatc acgatcccca
     2461 gcttcgggct cagtttggtg atcccgccgc gcagttcgga gttgccgtag gagtagacgg
     2521 tgtcgccgag gtcgccgacc gtgccgaccc cggtcgggcc gccgaagccg ggcaccgagg
     2581 ggttgacgtt ggcgacgtcg gaggccgcca gccggaccag cgcgaggtcg ttgtaggcgc
     2641 aggcgtcggg gttggtctca ccagcggcct gcatcgccag ccaggagttg tagacgagcg
     2701 tgccgggctg tttggcaccg gtcacctcga ccggggtgcc gagcggcagc gagccgctgg
     2761 tgcagccgtc ggtttcggtc tggccgccgg tgcccgagca gtgggcggcc tggccgatca
     2821 agaccgtgtc gccttctttg aagatgaagt ttgcggtgca ctggccgcct tcggtgcggg
     2881 tctgcacgcc ggggtggatt gccgccgttt ttgccggcgc ccaggcactg gccgccacgg
     2941 gcaaaatcag caccgccgcc accgccatca ccgtcgcgat caggccaagc acaagcccgt
     3001 aggtccctcg ccgcatcgaa tcctcctggt tcaggtgccc gcctcccggc ggcttgccgg
     3061 gatcgatgct acccataatt aagtagtcga aacaatgggc cccgcggagc cgagcgtttc
     3121 ggaactctcg cggaatggcc gtgttcacta ccgatgtgac tcgtcccgtc tacgtctttc
     3181 gccgcctcgt cgccctggtc gcggccctgg ttgcagtcag cgcgccagct gccgcggtcg
     3241 ccgcgcctgg cgacgtgacc acctacccgc tcgcgcccgg cgccgatccg cgcgggatcg
     3301 tcagcggccc gggcggcgcg ctctggttca cccagggcgg cgccggcggg ctcggccgga
     3361 ttaccaccgc gggagagatc gccggctttc ccgccgctcc gacgggtagc gctctgcagc
     3421 ttggcccgat cgccgtcggc cctgacggca acctctggta cggcggcctg ggcgggctcg
     3481 ggaggatgac ccccgccggt gtcgccgagt cgttcccgtt ttcggacttc cccaagcagc
     3541 tcgccgcggg ccccgatggc gccctctggt tcagcggcgt cgagaacggt cggctcggac
     3601 ggatcaccac cgcgggcgag ctcacctatg tcgagctgcc caacagcatc ggcccggccc
     3661 cggctgacca gaccgaagtg accggcatca cgaccggccc cgacggcaac gtctggttcg
     3721 gcagcgttaa cggccgctgt tattgcggta aaggcttgat tccaggggaa ggcaggatcg
     3781 gcacgatctc ggcgggcggc gtgccgagcg aaatcgcaat cccgggtggc ggccaaccgt
     3841 tgtcgatcgc ccccggccct gacggcgcga tctggttcac cttcggcctc ggcgcggtcg
     3901 gccgctacgc cggcggtcgc ttctcgatcg tggtcacgcg gtcgaccatc gccttccgca
     3961 gcctcgtcgc cggtgccgac ggcaacctct ggttcgcggt gggacggggg atcgggcgct
     4021 tgacgccgag cgggcacctg actgtcttca ggctcgccga gcgggcccgc tggttgacct
     4081 caggctccga cggcaacctc tggttcacca ccgccggggc ggtcggcaga ctcgagccgg
     4141 gcaagccagg ggtcgaactg atcccgccca agcgccgccc ccggaacggc aggctggcgg
     4201 tcgacctctt ctgcagcggc ggcggtgccg cggcttgcgc gggcagggcg aagctaatca
     4261 agaagacctt cctgcgccgc gagcacggcg aagcgaccta ccggacgcga ctgctcggcc
     4321 gcggccgcta ctccgtcccc ggcgaaagcg gcgggcggat ggtcgtacgg ctcaactcgc
     4381 tgggccgcaa gctggtccgc gagggcccgc tgagcgcgac gctggtcgcc aacgccggtg
     4441 ccggcgtcgg tgcccgcagc caggttcgcc tgcgtcggcc ataatcggcg catggaggtg
     4501 gcgatcgcag gcggtcacgg caagatcgcg atgctgctgg gcaaggcgct cgccgagcgc
     4561 ggcgacatcg tctggggcct gatccgcaac cctgaccaca aggacgacct gcaggcggcc
     4621 gggatcgagc cggtgctctg cgacctcgag gccggcgacg atgtcgccgc cggggtgcgc
     4681 ggtgccgaag cggtcgtctt tgctgcgggc gccggcccgg gcagtggcgc cgggcgcaag
     4741 cagacgatgg actacgaggg cgccgtgaag ctgatcgagg cggccaaggc cgagggcatc
     4801 cgccgctacc tgatggtcag ctcgatcggc gccggccgcg ccccggccga gggcggcgac
     4861 gtcttcggcg agtacctgcg ggcgaaggcg gcggccgacc aggcgctggc cgagagcggc
     4921 ctcgagtaca cgatcgtgcg cccgggcccg ctcaccgacg atccgcccag gggcttggtc
     4981 caggtcggcg tcgacctcga ccgcgtcgag ataccccgtg ctgacgtcgc tgctgtgctc
     5041 gccgcctgcc ttgactcccg ccacacgatc gaccgcacct tcgaagtggt cggcggcgac
     5101 atcgcgatcg acgccgcgct cgcctcgatc tagtcagacg gtagctgcgg cgccgctctg
     5161 caccgggtgg ccgccgtcgc gccaggcgcc gaccccgcgg ccgacgtgga cgacgtcgaa
     5221 gccctcgcgc tgcaggatcg aggcagcgag gccgctgcgt ttgccgcccc ggcagatcgc
     5281 cgcgatctgg cgctcgcggg ggatctcgtc gaagcgcccg gcgaggtcgc cgtaggggat
     5341 gtggagggag ccggggatgt gctcgcccgc gtactcggcg gcgttgcgca cgtcgaggat
     5401 cagcggcggc tgcgggccct cgaccctgct cgccagctcc tcggggtcga tcagctcgat
     5461 ccgctgcacc ggccgctcct cgacccgcca ggcggtcatc ccgccctcga ggaagccgcg
     5521 cacgcgcatc ccgaccgccg ccaacagctc ggcggcggcg cgctcgtcgc cgctggaggc
     5581 ggcgaccacg atcacgtcgg cgcccggcgg cgccacccgc gtgaccttgg tcgcgaagcc
     5641 ggtgtcgtag gaggaggcac tgagcgcgcc agcgatgtgg gcttcgtcga actgctcgtt
     5701 ggtgcgaccg tcgatcacca ccgcgccggc ggccacgcac tcctcgacct cgtgcgggct
     5761 catcggcgcc ggggtgccga agtcgtcgac cagagggccg cggttgagcg cgacgatctc
     5821 ggccacgttc ggcggtttgt cgccgatcga gccgaccgcg tcctcgacga actcggcctc
     5881 gtcgaggatc gcgagcgcct tgttgtggcg ccgctcgaag ccgatcgtcg aggagttctt
     5941 caggtcgagg ccggagccgc cgcagagcga gccgccgagg tggccgggcc agacctcgac
     6001 ctcgtcgtcg agggtgagca gccgctcgtg cagggtgtgg aacatctgcg cggcgccgtc
     6061 gcggggctcg atcgcgaggt cggggcgggc gacgtcgccg atgaacagcg agtcgccgct
     6121 gagcagcgcc cagggggtg
//
LOCUS       DASRIG010000318         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_687404, whole genome shotgun sequence.
ACCESSION   DASRIG010000318 DASRIG010000000
VERSION     DASRIG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687404"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFB52_08665"
     CDS             complement(<1..804)
                     /locus_tag="VFB52_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZO06446.1"
                     /translation="MNTAGGVVDCEQWQGHVIVCGLEGVGLRTVEQLRGAGVAVVVVD
                     DDPDEARAELVKGWGSAYMRATGSLDDELQLAGIAGARAVVCTQTNDLRNVETALLVR
                     DRREDIAVVAHLDNPTVGRAVEEATGEGSALDVAGLFAPAVVDACMLRTSHEVVLGGE
                     GFVAAEVTVKAPGSLRLLFGDLVPVGVVTGEGELIVCPGRDQQVASGDLVTVLGRPDE
                     LAAAEVGRPLDQEEPPRQRGGSVRQELRRISRTLAQGTDRAVGITLVLSL"
     gene            872..2377
                     /gene="zwf"
                     /locus_tag="VFB52_08670"
     CDS             872..2377
                     /gene="zwf"
                     /locus_tag="VFB52_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZO06447.1"
                     /translation="MADTDHLSSVPPASASHADPIEAVGNVAPPLPPHVILLFGATGD
                     LARRKLLPGLLRLYQAGLMPEFRIVGISLEDLSDEEFRFLAGSACGEFARHAYDEKLW
                     DDFSSRLSYVPLATARSSLAEVVVKAEHGLGEDVRLLHYLSVPPAAAADVVGLLGECG
                     LTERSRVIMEKPFGTDLASAQELNETVHEVLREDQIFRIDHFLGKEAALNILALRFAN
                     GLFEPIWNRDHIDHIQIDVPETLGVGSRAGFYEKTGAYRDMVVTHLMQILAFVAMEPP
                     TSLDSEPITEEKNKVFRSLRPLDPDWVVRGQYDGYTSEHGVEPESSTETFIALRNEID
                     NWRWSGVKFYLRTGKRMGDSGRIISVAFREPPQSMFPAHSRVGRYGPDHLTFDLDESS
                     RVSLSFYGKKPGMGMVLDKASMQFSLDETAYRGDTLEAYERLIRDAMAGDHTLFTTAR
                     DIERLWEISEPLLRDPPPVKPYAPGSWGPTQIGRLIAPNEWRLPFARRWRD"
     gene            complement(2388..3461)
                     /locus_tag="VFB52_08675"
     CDS             complement(2388..3461)
                     /locus_tag="VFB52_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06448.1"
                     /translation="MRRATCAITALLLAVLVSAPAAWGAGIPLSGGEAKLVFDRGFDK
                     ELRQEGVTVKALGPAKLKGRNLTLPVRLGTFEPTGNVVMSQSGGFRLTAGGKGVALRQ
                     LNLNSEKNRLSATLAGKRIALAELGGAKFTRNGFDARLEVKRLSLTRPGANALNRLLG
                     LPGLLKKGRSLGSIDALGIATSVPISFGQIAIGGPETAFSKLESIKVQMGIWGSTGSW
                     RAPGENYFLFDVAPTTLAPDASTGILEGGVNDGITMQIHEAPPREMLLRHPRIDLATR
                     ELSATLSPISTANPVTATIATLDYGAANFQIRPKVGAIELIGIRAISNQLIADQLNER
                     FATPGLFVAGETLARITVNLHAG"
     gene            complement(3458..4090)
                     /locus_tag="VFB52_08680"
     CDS             complement(3458..4090)
                     /locus_tag="VFB52_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZO06449.1"
                     /translation="MAEIRAVVSDFGGVLTTPLMTAFMSLQDEIGVSPEHFGTALRGL
                     TEEQGENPLFKMERGELSEADFLEQLGRGLEPLLDHEPHLHRFRELFIGGLDVNPPMI
                     GLMEELKATGLKMALLTNNVVEWEPVWRPMLPVDEIFEVVVDSGFVGMRKPEAGIYEL
                     TLERIGMTAPECVFVDDLKPNIDAAQELGFATVHFQDNEQAIAEIRAAVA"
     gene            complement(4083..4475)
                     /locus_tag="VFB52_08685"
     CDS             complement(4083..4475)
                     /locus_tag="VFB52_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06450.1"
                     /translation="MGGSGEIQRRSIGIVFSDGGLLALTSKLPAGGHEHGEEQVVAVL
                     CDPDAAPVEFEEALLSTEYGEDGVQRRATLELWPQIDDGHPLRGAGTLINSVTVKRDD
                     LDAEIAFFRWSVEGREGLGHYEVARADG"
     gene            4503..>4954
                     /locus_tag="VFB52_08690"
     CDS             4503..>4954
                     /locus_tag="VFB52_08690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06451.1"
                     /translation="MKESPATIRPADPERDAAACAAIYAPYVEDGPISFEERAPDAAA
                     MATRIAAHGSTHGWLVAERGGEVVGYAYACPHRARTAYRWSVDVSVYVAPGHTGEGVG
                     RAVYTELFERLRAQRFRMAFAGITLPNEASVALHESLGFAQVGTMREVG"
BASE COUNT          734 a         1769 c         1672 g          779 t
ORIGIN      
        1 cagcgagagg acaagggtga tgccgacggc gcgatccgtg ccctgggcga gggtgcggct
       61 gatccggcgc agctcctgcc gcaccgaccc cccgcgctgt cgaggcggct cctcctggtc
      121 gagtgggcgt cccacctcgg cggcggcgag ttcgtcgggc ctgcccagca cggtgaccag
      181 atcgccgctg gcgacctgct ggtcgcggcc ggggcagacg atcagctctc cctcgccggt
      241 gacgacgccg accgggacga ggtcgccgaa cagcaggcgc aacgagcccg gcgccttgac
      301 ggtgacctcg gcggcgacga agccctcgcc gccgaggacg acctcgtggc tggtccgcag
      361 catgcaggcg tcgaccacgg cgggggcgaa gaggccggcg acgtcgagcg cgctgccctc
      421 gccggtcgcc tcttcgaccg cgcgcccgac ggtcgggttg tcgaggtggg cgacgacggc
      481 gatgtcctcg cggcggtcgc gcaccagcag cgccgtctcg acgttgcgca ggtcgttggt
      541 ctgggtgcag acgacggcgc gggcgccggc gatcccggcc agctgcagct cgtcgtcgag
      601 gctgccggtc gcccgcatgt aggcgctgcc ccaacccttg accagctcgg cgcgggcctc
      661 gtcgggatcg tcgtcgacga cgaccacggc gacgccggcg ccgcgcagct gctcaaccgt
      721 gcgcaggccc accccctcga ggccgcagac gatcacgtgt ccctgccact gctcgcaatc
      781 gacgacgccg cccgctgtgt tcaagcgcgg caccctattc gcgtcacgcc gcgatcacaa
      841 cacctcttga cgcggcatct aagctcgctg cttggcggac actgatcacc tctcctccgt
      901 acctccggcc agcgccagcc acgctgaccc aatcgaggcg gtcgggaatg tggcgccgcc
      961 gttgccgccc cacgtaatcc tgctcttcgg ggcgaccggg gacctggcgc ggcgcaagct
     1021 gctgccgggg ctgttgcggc tctaccaggc ggggctgatg ccggagttcc ggatcgtcgg
     1081 gatctcgctg gaggatctga gcgacgagga attccgcttt ctcgccggca gcgcctgcgg
     1141 tgagttcgcc cgtcacgcct acgacgaaaa gctctgggac gacttctcca gccgactcag
     1201 ctacgtgccg ctcgcgaccg cccgcagcag cctcgccgag gtggtggtca aggccgagca
     1261 cgggctcggt gaggacgtgc ggttgctcca ctacctgagc gtgccgcccg cggccgccgc
     1321 cgacgtggtc gggctgctcg gcgagtgcgg gctgaccgag cgctcgcggg tgatcatgga
     1381 gaagccgttc gggaccgacc tggcgagcgc ccaggagctg aacgagacgg ttcacgaggt
     1441 cctgcgcgag gaccagatct tccgcatcga ccacttcctc ggcaaggagg cggcgctcaa
     1501 catcctcgcc ctgcgttttg ccaacgggct gttcgagccg atctggaacc gcgaccacat
     1561 cgatcacatc cagatcgacg tgccggagac gctcggggtc ggttcgcgtg ccggtttcta
     1621 cgagaagacg ggcgcctacc gcgacatggt cgtcacccac ctgatgcaga tcctcgcctt
     1681 cgtcgcgatg gagccgccga cctcgctgga ttcggagccg atcaccgagg agaagaacaa
     1741 ggtcttccgc agcctgcgcc cgctcgaccc ggactgggtc gttcgcggcc agtacgacgg
     1801 ctacacctcc gagcacgggg tggagcccga atcgagcacc gagaccttca tcgccctgcg
     1861 caacgagatc gacaactggc gctggagcgg ggtgaagttc tacctgcgca ccggcaagcg
     1921 gatgggggac agcggccgga tcatctcggt cgccttccgt gagccgccgc agagcatgtt
     1981 cccagcccac tcgcgggtcg gccgctacgg acccgaccac ctcaccttcg atctcgacga
     2041 gtcctcgcgc gtctcgctct ccttctacgg caagaagccc ggcatgggga tggtgctcga
     2101 caaggcgagc atgcagttct cgctcgacga aaccgcctac cgcggcgaca ccctcgaggc
     2161 ctacgagcgg ctgatccgcg acgcgatggc cggtgaccac accctcttca ccaccgcccg
     2221 cgacatcgag cgtctctggg agatctccga gccgctgctg cgcgatccgc cgccggtcaa
     2281 accgtatgca cccggctcct ggggtccgac ccagatcggc cgcctgatcg cgccgaacga
     2341 atggcgcctc ccgttcgccc gccgctggcg cgactaggcg tcgccgccta tccggcgtgc
     2401 aggttcaccg tgatccgggc aagcgtttcg ccggcgacga agagacccgg ggtggcgaac
     2461 cgttcgttca gctggtcggc gatgagttgg ttggagatcg cgcggatgcc aatcagttcg
     2521 atcgctccca ccttcgggcg gatctggaag ttggcggcgc cgtagtcgag cgtcgcgatc
     2581 gtcgccgtca ccgggtttgc ggtcgagatc gggctgagcg tggccgacag ttcgcgcgtg
     2641 gcgaggtcga tccgcgggtg gcgcagcagc atttcgcgcg gcggcgcttc gtggatctgc
     2701 atcgtgatcc cgtcgttcac accgccttcg aggatcccgg tcgacgcgtc cggcgcgagc
     2761 gtggtcggtg ccacgtcgaa caggaagtag ttttcgcccg gcgcccgcca cgaccccgtc
     2821 gagccccaga tccccatctg gactttgatc gattcgagct tcgagaacgc cgtttcgggt
     2881 ccgccgatcg cgatctggcc gaaggagatc ggaaccgagg tcgcgatgcc gagggcgtcg
     2941 atcgagccca gcgaccggcc tttcttcagc aggccgggga gcccgagtag gcggttgagc
     3001 gcgttcgccc caggtcgagt gaggctgagg cgtttgacct cgagtcgggc gtcgaagccg
     3061 ttgcgggtga atttcgcgcc gccgagctcg gcgagcgcga tccgcttgcc ggcaagggtc
     3121 gcgctgaggc gattcttttc gctgttgagg ttgagctgcc gcagtgcgac gcccttgccg
     3181 ccggcggtca gcctgaaccc gcccgattgc gacatgacca cgttgccggt cggttcgaac
     3241 gtacccaggc gcaccggcag agtcaggttt cggcccttca gcttcgccgg cccgagcgcc
     3301 ttgacggtga ccccttcctg gcgcagctcc ttgtcgaacc cgcggtcgaa cacgagcttc
     3361 gcctcgccac cgctgagcgg gatgccggct ccccaggccg ccggggcaga gacgagcact
     3421 gcgagcagca gcgccgtgat ggcgcaggtc gcgcgtctca tgcgaccgcg gcgcggatct
     3481 cggcgatcgc ctgctcgttg tcctggaagt ggacggtggc gaagccgagc tcctgggccg
     3541 cgtcgatgtt cggtttcaga tcgtcaacga agacgcactc gggcgcggtc atcccgatcc
     3601 gctccagggt cagctcgtag attcccgctt ccggcttgcg catcccgacg aagccggagt
     3661 cgacgacaac ttcgaagatc tcgtcgaccg gcagcatcgg ccgccagacc ggctcccact
     3721 cgaccacgtt gttggtcagc agcgccatct tcaggccggt cgccttcagc tcctccatca
     3781 acccgatcat cggcgggttg acgtcgagcc cgccgatgaa cagctcgcgg aagcggtgca
     3841 ggtggggttc gtggtcgagc agcggttcga gcccgcggcc gagctgctcg agaaagtcgg
     3901 cttcgctgag ctcgccgcgc tccatcttga acagcgggtt ctcgccctgt tcctcggtca
     3961 gcccgcgcag ggcggtgccg aagtgctcgg gggaaacgcc gatctcgtcc tgcagcgaca
     4021 tgaaggcggt catcagcggc gtcgtcagga caccgccgaa gtcggagacg acggcgcgga
     4081 tctcagccat cggcgcgcgc gacctcgtag tggccgagcc cctcgcggcc ctcgaccgac
     4141 cagcggaaga aggcgatctc ggcatcgagg tcgtcgcgct tcaccgtcac cgagttgatc
     4201 agggtgccgg cgccgcgcag cgggtgcccg tcgtcgatct gcggccagag ctccagcgtc
     4261 gcccgccgct gcaccccgtc ctcgccgtac tcggttgaga gcagggcctc ctcgaactcg
     4321 accggggccg cgtcgggatc gcagagcacc gcgaccacct gctcctcgcc gtgctcgtgg
     4381 ccgcccgccg gcagcttcga ggtcagcgcc aggagaccgc cgtcggagaa gacgatgccg
     4441 atcgagcgcc gctggatctc gccggagcca cccatgccct cggcagccta ttgtcctggc
     4501 ccttgaaaga gagcccggcc accatccgcc cagccgaccc cgagcgggac gccgccgcct
     4561 gcgccgcgat ctacgccccc tacgtcgagg acggcccgat ctccttcgag gagcgtgccc
     4621 ccgacgccgc cgcaatggcg acccggattg ccgcccacgg ctccacccac ggctggctgg
     4681 tcgccgagcg cgggggagag gtggttggct acgcctacgc ctgcccgcac cgcgcccgca
     4741 ccgcctaccg ctggtcggtc gacgtctccg tctacgtcgc ccccggccac accggcgagg
     4801 gagtcggccg cgccgtctac acggagctgt tcgagcgcct gcgcgcccag cgcttccgca
     4861 tggccttcgc cgggatcacc ctgcccaacg aggcgagcgt cgccctgcac gagagcctcg
     4921 gcttcgcaca ggtcgggacg atgcgcgagg tcgg
//
LOCUS       DASRIG010000319         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3549913, whole genome shotgun sequence.
ACCESSION   DASRIG010000319 DASRIG010000000
VERSION     DASRIG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549913"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFB52_08695"
     CDS             complement(<1..535)
                     /locus_tag="VFB52_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06452.1"
                     /translation="MDVSKLGFHVITSPDPAAIANHYETALQLSRTGSEGDTIFLTPH
                     GDHHSVAIRKGEPNGRAGLGFEIHGTLEEAASGLDSVGIPHERRSDSEPGIGDALMIE
                     EPFSGTSIALYKAQESSGEGSVVGPRPSKLGHIASYVSDLSAGHEFYLGVLGFRWGDT
                     IGDFFSFLRCNADHHAVN"
     gene            737..1726
                     /locus_tag="VFB52_08700"
     CDS             737..1726
                     /locus_tag="VFB52_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO06453.1"
                     /translation="MQGKIAVEEHFVTPQLKDTIFGSIGWEEAEWEAMAGALQETDEV
                     RLADMDASGIEVAAVSLAAPCIQDIVDPAQATEAAAGANDALAAVVAAHPDRYVGLAA
                     LGLQDPEAAADELERCVTELGFRGALANGYSSVGDLDTAAYYDEPQYEVFWERVARLG
                     VPFYLHPRNPLPNSRYYQGREMLLGPTWAFTVETATHSLRLMVSGLFDRYPELNVIIG
                     HMGELLPFAVARTEQRLSHIPTLKLDRGPQDLLRENFFITTSGNFHTQSLIGAILQVG
                     ADRILFAADYPFEKTAEAAEWFDNVAISENDRQKIGRDNAAALLGIDSLAGVS"
     gene            1731..2642
                     /locus_tag="VFB52_08705"
     CDS             1731..2642
                     /locus_tag="VFB52_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZO06454.1"
                     /translation="MSAEVERPVSPEEITERVLARFESTPDPRLREIMRALVTHLHGF
                     VTDTGLTEAEWAEAIAVLTATGDITDDKRQEFILWSDTLGVSMLVDALAHPAPNGATE
                     STVLGPFYVPGSPLREHGADISEQEAGTPAWVHGRVLDLDGNPIAGAELDVWQNGDNQ
                     LYAVQDAEAPDDHLRGRFMTDAAGRFAFRAVRPVPYPIPDDGPVGRMLNATDRHPWRP
                     AHIHVVVRAEGFASVTTHIFDATSTYLDDDTVFAVKPSLLREFVDRAADDPERPAGVE
                     EPWVSLELDLVLAPGESEIPADLGRTH"
     gene            2650..3732
                     /locus_tag="VFB52_08710"
     CDS             2650..3732
                     /locus_tag="VFB52_08710"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HZO06455.1"
                     /translation="MPTSLATRVSYEALPGRVVFGAGAVGELAAEVELLGGRRVILFD
                     GLFDAMLVERLERELGERHAGTIGEVAQHVPAELAASALARARELEADCLVALGGGSA
                     TGVAKAVAKETGLPILAVPTTYAGSEMTPIWGITADARKTTGRDLAVLPRTVIYDPEL
                     TLSLPPLASAASGMNAVAHCVEAMWTAAANPVTDAVAAEGITLLAAGLRGSVHEPRDL
                     RARALALQGAWLGGTALAVGGTGLHHKICHVLGGTFDLPHAEVHAIVLPWVVEHYRAA
                     APAALARVARALDAEDAVTGLRELGRDLGLAAGLADFGLRPADIELAAELAAAVAPDV
                     PAPTNKEEIVAILESAMTMGTTGREQ"
     gene            3733..4506
                     /locus_tag="VFB52_08715"
     CDS             3733..4506
                     /locus_tag="VFB52_08715"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZO06456.1"
                     /translation="MADLDGRVAVVTGGAKGIGLGICECLSEQGATVVVADIDGAAAE
                     QSAASLPGASLAVATDVSDSGSVAALADKVKEEYGRLDILVNNAGVGPKPTPIVELSE
                     EEWDRVMNINARGVFLVNRALVPLLVASEAGRLINISSIVGQSGFALVSQYVASKFAV
                     TGMTQCLAHELAPNGVTVNSIHPGILETELHSNVVKLFSAIQGQSEEETWDWFKERIP
                     LSKFQTPRDIGEMAAFLASDRASNITGAAFNVDGGWEMH"
BASE COUNT          688 a         1634 c         1594 g          637 t
ORIGIN      
        1 tgttgaccgc gtggtggtcg gcgttgcagc gcaggaagct gaagaagtcg ccgatcgtgt
       61 cgccccaacg gaagccgagc acgccgaggt agaactcgtg gccggccgag aggtcgctca
      121 cataggaggc gatgtggcct agcttcgacg gccgcggccc gacgaccgaa ccctcgccgc
      181 tcgactcctg cgccttgtag agggcgatcg aggtgccgct gaatggctcc tcgatcatga
      241 gcgcgtcgcc gatgccgggt tcggagtcgc tgcggcgctc gtgggggatc ccgaccgagt
      301 cgagcccgga ggcagcttcc tcgagggtgc cgtggatctc gaagccgagg ccggcgcgac
      361 cgttcggctc gcccttgcgg atcgcgaccg agtggtggtc accgtgcggg gtgaggaaga
      421 tcgtgtcgcc ctcgctgccg gtccgcgaga gctgcagcgc ggtctcgtag tggttggcga
      481 tcgccgccgg gtcgggcgag gtgatcacgt gaaagccgag cttcgagacg tccaccgggg
      541 gcttctccgg cgtctccgtt cgcagcgcgg gggtacctga ctccattgcc tctcgacctc
      601 ctcagtcgtc cctcgttcgt tcgaattccc accgcgtacg ttacacgatg cgtagtcagt
      661 tgactaatgc gtaacggtgt gcgaggatcg ccgaacgggc gtgggaggcc gcgaaagcac
      721 gacttcgagg agagagatgc aaggcaagat cgccgtcgag gagcacttcg taacgccgca
      781 gttgaaggac acgatcttcg gcagcatcgg ctgggaggag gccgagtggg aggcgatggc
      841 cggtgccctg caggagaccg acgaggtccg gctcgccgac atggacgcct ccgggatcga
      901 ggtcgccgcg gtctcgctcg ccgccccctg catccaggac atcgtcgacc ccgcgcaggc
      961 gaccgaggcc gccgccggcg ccaacgacgc gctcgccgcc gtggtcgccg ctcaccccga
     1021 ccgctacgtc ggcctcgccg cgctcggcct gcaggacccc gaggccgccg ccgacgagct
     1081 cgagcgctgc gtcaccgagc tcggcttccg cggcgccctc gccaacggct actcgagcgt
     1141 cggcgacctc gacaccgccg cctactacga cgaaccgcag tacgaggtct tctgggagcg
     1201 ggtcgcccgg ctcggcgtcc ccttctacct gcacccgcgc aacccgctgc cgaacagccg
     1261 ctactaccag gggcgcgaga tgctgctcgg gccgacctgg gcgttcacgg tcgagaccgc
     1321 gacccactcg ctgcggctga tggtcagcgg cctcttcgac cgctacccgg agctgaacgt
     1381 gatcatcggg cacatgggcg agctgctccc gttcgcggtc gcgcgcaccg aacagcgcct
     1441 ctcccacatc ccgacgctga agctcgaccg cggcccgcag gacctgctgc gcgaaaactt
     1501 cttcatcacg accagcggaa acttccacac gcagtcgctg atcggcgcga tccttcaggt
     1561 cggcgccgac cgcatcctgt tcgccgccga ctaccccttc gagaagaccg ccgaggccgc
     1621 cgagtggttc gacaacgtcg cgatcagcga gaacgaccgc cagaagatcg gccgcgacaa
     1681 cgcggcggcg ctgctcggga tcgacagcct cgccggggtg tcctgagccg atgagcgccg
     1741 aggtcgagcg gccggtctcc cccgaggaga tcaccgagcg ggtcctggcc cgcttcgagt
     1801 cgaccccgga cccgcgcctg cgcgagatca tgcgggcgct ggtcacccac ctgcacgggt
     1861 tcgtcaccga caccggcctg accgaggcgg agtgggcgga ggcgatcgcg gtgctgaccg
     1921 ctacgggaga catcaccgac gacaaacgcc aggagttcat cctctggtcg gacacgctcg
     1981 gggtctcgat gctggtcgac gcactcgccc acccggcgcc gaacggggcg accgagtcga
     2041 ccgtgctcgg ccccttctac gtgcccggct cgccgctgcg cgagcacggc gccgacatct
     2101 ccgagcagga ggcggggacg ccggcctggg tccacggccg cgtgctcgac ctcgacggta
     2161 acccgatcgc cggggccgaa ctcgacgtct ggcagaacgg cgacaaccag ctctacgcgg
     2221 tccaggacgc cgaggccccc gacgaccacc tgcgcgggcg cttcatgacc gacgccgcgg
     2281 ggcgcttcgc cttccgggcg gtgcgcccgg tcccctaccc gatccccgac gacggcccgg
     2341 tcgggcgcat gctgaacgcg accgatcgcc acccctggcg gcccgcccac atccacgtcg
     2401 tcgtccgcgc cgagggcttc gccagcgtca ccacccacat cttcgacgcg accagcacct
     2461 acctcgacga cgacaccgtg ttcgcggtca agccctcgct gctgcgcgag ttcgtcgacc
     2521 gcgccgccga cgacccggag cggccggccg gggtcgagga gccctgggtc tcgctggagc
     2581 tggacctggt gctggcgccg ggcgagagcg agatccccgc cgacctcggg cgcactcatt
     2641 aggacttcga tgccgacctc gcttgccacc cgggtctctt acgaagcgct cccgggtcgc
     2701 gtcgtcttcg gcgccggcgc ggtcggcgag ctggcggccg aggtcgagct gctcggcggc
     2761 cgccgcgtga tcctcttcga tggtctcttc gacgcgatgc tggtcgagcg cttggagcgc
     2821 gagctggggg agcgccacgc gggcacgatc ggggaggtcg cccagcacgt gccggcggag
     2881 ctggcggcga gcgcgctcgc ccgcgcccgc gagctcgagg ccgactgcct ggtcgcgctc
     2941 ggcggcggct ccgcgaccgg ggtggcgaag gcggtggcga aagagaccgg gctgccgatc
     3001 ctcgccgtcc ccaccaccta cgccggctcg gagatgacgc cgatctgggg gatcaccgcc
     3061 gacgcgcgca aaaccaccgg ccgcgacctc gcggtgctgc cgcgcaccgt gatctacgac
     3121 cccgagctga ccctcagcct gccgccgctg gctagcgccg cgagcgggat gaacgcggtc
     3181 gcgcactgcg tcgaggcgat gtggacagcg gccgccaacc cggtcaccga cgcggtcgcg
     3241 gcggaaggga tcacgctgct ggcggcgggc ctgcgcggct ccgtgcacga accgcgtgac
     3301 ctgcgggcgc gcgccctggc cctgcagggc gcctggctcg gcggcaccgc gctcgcggtc
     3361 ggcggcaccg ggctgcacca caagatttgc cacgtgctcg gcggcacctt cgacctcccg
     3421 cacgcggagg tccacgcgat cgtcctgccc tgggtggtcg agcactaccg cgcggcggcg
     3481 ccggcggcgc tcgcccgggt ggcgcgggcg ctcgacgccg aggacgcggt caccgggctg
     3541 cgcgagctgg gccgcgacct cggcctcgcc gccggcctcg ccgacttcgg gctgcggccc
     3601 gccgacatcg agcttgccgc ggaactggcg gcggcggtgg caccggacgt gccggcgccg
     3661 accaacaagg aagagatcgt cgcgatcctc gagagcgcga tgaccatggg aactacggga
     3721 agggagcagt aaatggctga cctcgacgga agggttgccg tcgtcaccgg cggtgcaaaa
     3781 ggaatcgggc tcgggatctg cgaatgcctg agcgagcagg gggcgacggt cgtcgtcgcc
     3841 gacatcgacg gcgccgcggc cgagcagagc gccgcgagcc tgccgggggc ctcactggcg
     3901 gtcgccaccg acgtcagcga ctccggctcg gtcgccgcgc tcgccgacaa ggtgaaggag
     3961 gagtacggcc ggctcgacat cctcgtcaac aacgccggcg tcggtcccaa accgaccccg
     4021 atcgtcgaac tgagcgagga ggagtgggac cgggtgatga acatcaacgc ccgcggcgtc
     4081 ttcctcgtca accgggcgct ggtgccgctg ctggtcgcct ccgaggcggg ccgcctgatc
     4141 aacatctcct cgatcgtcgg ccagtcgggc ttcgccctgg tctcccagta cgtcgcctcg
     4201 aagttcgcgg tcaccgggat gacccagtgc ctcgcgcacg agctggcgcc gaacggcgtc
     4261 accgtcaact cgatccaccc cggcatcctc gagaccgagc tgcactcgaa cgtggtcaag
     4321 ctgttcagcg cgatccaggg ccagtcggag gaggagacct gggactggtt caaagagcgg
     4381 atcccgctga gcaagttcca aaccccgcgc gacatcggcg agatggcggc cttcctggcc
     4441 tccgaccggg cgagcaacat cacgggagcg gccttcaacg tcgacggcgg ctgggagatg
     4501 cactgacggg cgtacttcgg gatcgcatgc ggggaggggt cgggtgactc cct
//
LOCUS       DASRIG010000320         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3826873, whole genome shotgun sequence.
ACCESSION   DASRIG010000320 DASRIG010000000
VERSION     DASRIG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826873"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1268
                     /gene="tuf"
                     /locus_tag="VFB52_08720"
     CDS             84..1268
                     /gene="tuf"
                     /locus_tag="VFB52_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZO06457.1"
                     /translation="MSKEKFERDKPHVNVGTIGHIDHGKTTLTAAITKTLSTVGGAEA
                     HSFEEIDNAPEEKERGITIATSHVEYQTENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGVMPQTREHILLARQVNVPHVVVFLNKIDQVDDDELVELVEEEVKDLLNE
                     YDFPGEETPIVKGSALKALEGDEEAQKAVFELAAALDSYIPEPVRELDKPFLMPIEDI
                     FSITGRGTVATGRVEQGIVNTGDEVEIVGIKDTTKTTVTGVEMFRKILDEGQAGDNIG
                     ALLRGTKREEIERGQVLCKPGSITPHTKFKAEVYCLKKEEGGRHTPFFSGYRPQFYFR
                     TTDVTGIANLPDGTEMVMPGDNVEMTIELIQPIAMDPGLRFAIREGGRTVGSGVVSEV
                     IE"
     gene            1439..1750
                     /gene="rpsJ"
                     /locus_tag="VFB52_08725"
     CDS             1439..1750
                     /gene="rpsJ"
                     /locus_tag="VFB52_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZO06458.1"
                     /translation="MAAQKIRIRLKAYDHDAIDRAAREIVETAERTGASVSGPVPLPT
                     EKNVYCVIRSPFKDKDSREHFEIRTHKRLIDILQPSPKTVDSLQRLDSLPAGVDIEIK
                     L"
     gene            1755..2390
                     /gene="rplC"
                     /locus_tag="VFB52_08730"
     CDS             1755..2390
                     /gene="rplC"
                     /locus_tag="VFB52_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZO06459.1"
                     /translation="MAAILGKKLGMTQVFQEDGTVVPVTVIEAGPCKVTAVREADRDG
                     YQAVQLAFGEVKPGKLTQAEEGHLKKAGSAPMRHLVEFRDEDFGGEDGEGPKIGDDVT
                     VTTFEAGQLVKVAGTSIGKGFQGGIKRHNFSRGPVSHGSHNVRAPGSIGASAFPARVF
                     KGMRMPGQMGNKRVTQKGLEIADIDADRNLLLIKGSVPGSKNGIVEVRTDG"
     gene            2383..2994
                     /gene="rplD"
                     /locus_tag="VFB52_08735"
     CDS             2383..2994
                     /gene="rplD"
                     /locus_tag="VFB52_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZO06460.1"
                     /translation="MAKATVLGKTTKADLPKEIFGEPFHQSLVHEAARADMAARRRGT
                     ASTLTRGEVAFTTAKAWRQKGTGRARVGALGVSSRYGGGVTFGPKPRHYTVKVNRKAR
                     RRALRSALSVHAERDSVAVLDASSFETPSTKDAAAALAKWAAKSPTLVVLTAEEVGAM
                     KSFRNIERVHVLEVGATGVADVIAAASLVVSETALKALEERLG"
     gene            2994..3287
                     /gene="rplW"
                     /locus_tag="VFB52_08740"
     CDS             2994..3287
                     /gene="rplW"
                     /locus_tag="VFB52_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZO06461.1"
                     /translation="MDARTILIRPVISEKSYALIAEGKYTFRVDDRAHKTQIAQAVEE
                     VFGVSVAEVRTAKVRSKPKRRGLIKGKTRAWKKAIVQLSPGERIELFEGGQTA"
     gene            3299..>3725
                     /gene="rplB"
                     /locus_tag="VFB52_08745"
     CDS             3299..>3725
                     /gene="rplB"
                     /locus_tag="VFB52_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZO06462.1"
                     /translation="MPNRKTKPTSPGRRFATYPLREQLTASEPERKLTEGLKKSGGRN
                     VNGRVTSRHRGGGAKRRYRKIDFKRLKDGVPAKVATIEYDPNRSCYIALLHYADGHKD
                     YILAPATLAVGDSVKSGPEADIKPGHAMALGQMPTGTIVH"
BASE COUNT          754 a         1283 c         1190 g          498 t
ORIGIN      
        1 aaacggcctg ccgggcgacc gcccacagac tcaaccactt agatcagaca gaacacaact
       61 ttcgaaaggc gaaacggaga gagatgtcca aggagaaatt cgagcgcgat aagccgcatg
      121 tgaacgtcgg caccatcggt cacatcgacc acgggaagac gacgctgacg gccgcgatca
      181 ccaaaaccct ctccaccgtg ggcggtgccg aggcgcatag cttcgaggag atcgacaacg
      241 ctccggagga gaaggagcgc gggatcacga tcgcgacctc gcacgtcgag taccagaccg
      301 agaaccgcca ttacgcccac gtcgactgcc cgggccacgc cgactacgtg aagaacatga
      361 tcaccggcgc cgcgcagatg gacggcgcga tcctggtcgt ctcggccgcc gacggcgtga
      421 tgccgcagac ccgcgagcac atcctgctcg cccgtcaggt caacgtcccg cacgtggtcg
      481 tcttcctcaa caagatcgat caggtcgacg acgacgagct ggtcgagctc gtcgaagagg
      541 aggtcaagga cctgctcaac gaatacgact tcccgggcga agagaccccg atcgtcaaag
      601 gctcggcgct gaaggcgctg gaaggcgacg aggaggccca gaaagccgtc ttcgagctgg
      661 ccgcggcgct cgactcctac atcccggagc cggtccgcga gcttgacaag cccttcctga
      721 tgccgatcga ggacatcttc tcgatcaccg gccgcggcac cgtcgccacc ggccgcgtcg
      781 agcagggcat cgtcaacacc ggcgacgagg ttgagatcgt cggtatcaag gacacgacca
      841 aaaccaccgt caccggcgtc gagatgttcc gcaagatcct cgacgagggt caggccggcg
      901 acaacatcgg cgccctcctg cgcggtacca agcgcgagga gatcgagcgc ggccaggttc
      961 tctgcaagcc cggctcgatc acgccgcaca ccaagttcaa agccgaggtc tactgcctga
     1021 agaaggagga gggtggtcgc cacactccgt tcttctccgg ctaccggcct cagttctact
     1081 tccggaccac cgacgtgacc gggatcgcca acctccccga cgggacggag atggtgatgc
     1141 cgggcgacaa cgtcgagatg acgatcgagc tgatccagcc gatcgccatg gatccgggcc
     1201 tgcggttcgc gatccgcgag ggtggccgca ccgtcggctc gggggttgtc tccgaggtca
     1261 tcgagtaggc gcaccaaacg ggacgggcag ggtgcccgtc cctccgtcgc ctctggcggc
     1321 gggacaactg caaagcacat acgaccgggg cggcgcccca aatcaaaggg gccccgccct
     1381 ctttgcgcgg taaagcgaag aagcccaaga cgagagaaac ggcagcaaag tggcagcgat
     1441 agcagcacaa aaaattcgaa tcaggctcaa ggcgtacgac catgacgcca tcgatcgcgc
     1501 tgcccgcgag atcgtcgaga ccgctgagcg caccggcgcc agcgtttccg gtccggtgcc
     1561 gttgccgacc gagaaaaacg tctactgcgt gatccgctcc ccgttcaagg acaaggactc
     1621 gcgcgagcac ttcgagatcc gcacccacaa gcggctgatc gacatcctcc agccgtcgcc
     1681 gaaaaccgtc gactcgctgc agcgtctcga ctcgctgccg gccggcgtcg acatcgagat
     1741 caagctctag gacgatggct gcgattctcg gcaagaagct cggcatgacc caggtcttcc
     1801 aggaggacgg aaccgtcgtg ccggtgaccg tgatcgaagc cggtccctgc aaggtgaccg
     1861 cggtgcgcga ggccgaccgc gatggctacc aggctgtgca gctcgccttc ggcgaggtca
     1921 agccgggcaa gctcacgcag gccgaggagg gtcacctgaa gaaggccggc tccgcgccga
     1981 tgcgtcacct ggtcgagttc cgcgacgagg acttcggtgg cgaggacggc gagggtccga
     2041 agatcggcga cgacgtcacc gtcaccacct tcgaggcggg ccagctggtg aaggtcgccg
     2101 ggacctcgat cggcaagggc ttccagggcg ggatcaagcg ccacaacttc agccgcgggc
     2161 cggtcagcca cggctcccac aacgtccgcg cgcccggctc gatcggcgcc agcgccttcc
     2221 ccgcccgcgt cttcaaaggg atgcggatgc ccggccagat gggcaacaag cgggtcaccc
     2281 agaagggcct cgaaatcgcc gacatcgacg ccgaccgcaa cctgctgctg atcaagggct
     2341 cggtgcccgg ctccaagaac ggaatcgttg aggtgaggac cgatggctaa agccaccgtg
     2401 ctcggcaaaa ccaccaaagc cgatctgccc aaagagatct tcggcgagcc cttccaccag
     2461 tcgctggtgc acgaggccgc gcgcgctgac atggctgccc gccgccgcgg taccgcttcg
     2521 accctgaccc gcggtgaggt tgctttcacc accgccaagg cctggcgcca gaagggcacc
     2581 ggccgcgccc gcgtcggcgc gctcggagtc tccagccgct acggcggtgg cgtcaccttc
     2641 ggcccgaagc cgcgccacta cacggtcaag gtcaaccgca aagcccgccg gcgcgctctg
     2701 cgctcggccc tctcggtgca cgccgagcgc gactcggtcg ccgtcctcga cgcttccagc
     2761 ttcgagacgc cttcgaccaa agacgccgcc gcggcgctcg ccaaatgggc ggccaagagc
     2821 ccgaccctgg tcgtgctcac cgctgaggag gtcggggcga tgaagagctt ccgcaacatc
     2881 gagcgggttc acgtgctcga ggtcggcgcc accggcgtcg ccgacgtgat cgccgccgcc
     2941 tcgctggtcg tttccgagac cgcgctgaag gcgctcgaag agaggctcgg ctgatggacg
     3001 cgcgcacgat tttgatccgc ccggtgatct cggagaagag ctacgccctg atcgccgagg
     3061 gcaagtacac cttccgcgtc gacgaccgcg cccacaagac ccagatcgcg caggcggtcg
     3121 aagaggtctt cggcgtctcg gtcgccgagg tgcggaccgc caaggtgcgc tcgaagccga
     3181 agcgccgcgg cctgatcaaa ggcaagaccc gcgcctggaa gaaggcgatc gtccagctct
     3241 cgccgggcga acggatcgag ctgttcgagg gcggacagac cgcctaggag actgaggaat
     3301 gccgaaccgc aaaaccaaac cgaccagccc gggccgccgc ttcgcgacct acccgctgcg
     3361 cgagcagctg accgcgtccg agcccgagcg caagctgacc gaggggctga aaaagtccgg
     3421 tggccgcaac gtcaacggcc gcgtcacctc ccgccaccgc ggcggcggcg ccaagcggcg
     3481 ctaccggaaa atcgacttca agcgactcaa ggacggcgtg cccgcgaagg tcgcgacgat
     3541 cgagtacgac ccgaatcgca gctgctacat cgccctgctc cactacgccg acgggcacaa
     3601 ggactacatc ctcgccccgg cgacgctggc ggtcggcgac tcggtcaaat cgggccccga
     3661 ggccgacatc aaaccgggcc acgcgatggc gctgggccag atgccgaccg gcacgatcgt
     3721 ccaca
//
LOCUS       DASRIG010000321         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2232256, whole genome shotgun sequence.
ACCESSION   DASRIG010000321 DASRIG010000000
VERSION     DASRIG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232256"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..480)
                     /locus_tag="VFB52_08750"
     CDS             complement(61..480)
                     /locus_tag="VFB52_08750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZO06463.1"
                     /translation="MSAPFVHEFRVRWGECDPQGIVFNAHYVAYFDEAIVALWREAFD
                     SPYGEMVDEEGIDMVVAEVNVQYRASARPEEMIRVELTVEALGETSMTSRLDVMRGEE
                     LLVKGRMRHVFVDTASWTKTPIRDRIRAGLEPYHAYA"
     gene            518..1606
                     /gene="proB"
                     /locus_tag="VFB52_08755"
     CDS             518..1606
                     /gene="proB"
                     /locus_tag="VFB52_08755"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZO06464.1"
                     /translation="MPLVVKLGSSIVAADDGELRTDVLDSVCAQVSALERGGERVVMV
                     TSGAIARGMRLMGLPVRPRAMDELQGASAVGQGDLFRAYESRLAKGGTRAAQVLLTAA
                     DIAARTNYLNARQTLQRLLQWEVVPVINENDTTATDEITFGDNDFLAAQVALLLDARL
                     LVLLTNVDGLHTGDPSLDAEAKLIEQVGGAAELAELQIGDRTSAFGSGGMRSKVAAAE
                     MASEAGIPAVICNGTVDGTLATAAAGGQVGTGFAAQAGRDSSFKLWLKYAKPAKGRLL
                     VDAGAARVLRERGSSLLPVGIAGVEGGFEAGDAVDVASDGELIGKGICEYSSRELAQV
                     IGLQSEAVRELLPHAADEVIHRDRFVLV"
     gene            1630..2895
                     /locus_tag="VFB52_08760"
     CDS             1630..2895
                     /locus_tag="VFB52_08760"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZO06465.1"
                     /translation="MATTATTVAQSCAAAKRAARPLASASTEAKNAALEATARLLEER
                     TAEILEANAGDLADESAAGLNAALKDRLTLTPERVAAMAEGVRVVAALPDPVGEELEH
                     KTLENGIDLRKVRVPLGVVAVVYEARPNVTIDCAALTIKSGNAIVLRGSHYAERSNGA
                     LAGIVREALAEAGLPEDAVVLHAGGGHGELAELATQEGLVDLLIPRGGEGLKNAIKDV
                     ATVPVMYAAAGNCHVYVHAEADPEMARQIALNAKVQRPGVCNAAETLLLDAAIADELG
                     PAILGELAAAGVELVGDERARGLGGEAEVGVATDEDWDREYLELKLAVAVVDSVEEAI
                     EHVNAHGTGHSEAIVTGSAQAADEFVAGVDAAAVYVNASTRFTDGFEYGMGAEIGNST
                     QKLHARGPIGMRELTTFKYVARGDGQIRG"
     gene            2888..>3301
                     /locus_tag="VFB52_08765"
     CDS             2888..>3301
                     /locus_tag="VFB52_08765"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZO06466.1"
                     /translation="MADGRNGGIGVLGSAFNPPHLGHLALAQVALWQLGLDEVILVPT
                     GTAPHKRIADDPGREERMAMTRLAAADDSRFSVSALEVEREGPSYTYETLGLLAEERP
                     ETELVFVMGADAAVGLESWREPERVVGLARLAVARR"
BASE COUNT          493 a         1121 c         1224 g          463 t
ORIGIN      
        1 aacccccgct tgcgtcaatc gcgacccccc cagattcgag ggggtcgcga ttggcacggg
       61 ctaggcatag gcgtggtagg gctcgagacc cgcgcggatc cgatcccgga tcggcgtttt
      121 cgtccagctc gcggtgtcga cgaagacgtg gcgcatgcgc cccttgacca gcagctcctc
      181 gccgcgcatc acgtcgaggc ggctggtcat gctggtttcg ccgagcgcct cgaccgtcag
      241 ctcgacccgg atcatctctt ccggccgggc ggaggcgcgg tactggacgt tgacctcggc
      301 gacgaccatg tcgatcccct cttcgtcgac catctcgccg tagggggagt cgaaggcctc
      361 gcgccagagg gcgacgatcg cctcgtcgaa gtaggcgacg tagtgggcgt tgaagacgat
      421 cccctggggg tcgcactcgc cccagcggac gcggaattcg tgcacgaagg gcgcgctcat
      481 gggccgagcc tattgcccct cgtacacttg ccccgccatg cccctggtcg tcaagttggg
      541 atcttcgata gtcgccgccg acgatggcga gctgcgcacc gacgtgctcg actccgtctg
      601 cgcgcaggtg agcgcgctgg agcgcggggg ggagcgggtc gtgatggtca cctcgggcgc
      661 gatcgcgcgc gggatgcggc tgatgggcct gccggtgcgc ccgcgggcca tggacgagct
      721 gcagggcgcc tccgcggtcg gtcagggcga tctcttccgc gcttacgaga gccgcctcgc
      781 caagggcggc acgcgcgccg cccaggtgct gctgaccgcc gccgacatcg ccgcccgcac
      841 caactacctg aacgcgcgcc agaccctgca gcggctgctg cagtgggaag tggtgccggt
      901 gatcaacgag aacgacacca ccgccaccga cgagatcacc ttcggcgaca acgacttcct
      961 cgccgcccag gtggcgctgc tgctcgacgc gcgcctgctc gtgctgctga ccaacgtcga
     1021 tgggctgcac accggcgacc cctccctgga cgccgaggcg aagctgatcg agcaggtcgg
     1081 cggggccgcc gagctggccg agctgcagat cggcgaccgc acctcggcct tcggctcggg
     1141 cgggatgcgc agcaaggtcg ccgccgccga gatggcgagc gaggccggga tcccggcggt
     1201 gatctgcaac ggcacggttg acggcacgct ggcgaccgcg gccgccggcg ggcaggtcgg
     1261 caccggcttc gccgcccagg ccggtcgcga ctccagcttc aagctctggc tcaaatacgc
     1321 gaagccggcg aagggacggc tgctcgtcga cgccggcgcc gcccgcgtcc tgcgcgagcg
     1381 cggctctagt ctgctgccgg tcgggatcgc cggggtcgaa ggtggcttcg aagccggcga
     1441 cgcggttgac gtggccagcg acggcgagct gatcggcaaa ggcatctgcg agtattcctc
     1501 gcgcgagctg gcccaggtga tcggcctgca gagcgaggcg gtgcgcgagc tgctcccgca
     1561 cgccgccgac gaggtgatcc accgcgatcg cttcgtactt gtttagccac ctagctaccc
     1621 ttgaccgtta tggctacgac cgcgaccacc gtcgcccaga gctgtgcggc cgcgaagcgc
     1681 gccgcgcggc cgctggcgag cgcctcgacc gaggctaaga acgccgccct cgaggcgacc
     1741 gcgcggctgc tcgaagagcg cacagcggag atcctcgagg cgaacgccgg cgacctcgcc
     1801 gacgagagcg cggctggcct gaacgcggcg ctgaaggatc ggctgacgct gaccccggag
     1861 cgggtcgcgg cgatggccga gggcgtgcgc gtggttgccg cgctgcccga tccggtcggc
     1921 gaggagctcg aacacaaaac tttggagaac gggatcgacc tgcgcaaggt gcgggtgccg
     1981 ctgggcgtgg tcgcggtcgt ctacgaggcg cgcccgaacg tgacgatcga ttgcgcggcg
     2041 ctgacgatca agagcggcaa cgcgatcgtc ctgcgcggct cccactacgc cgagcgctcc
     2101 aacggggcgc tggccgggat cgtccgcgag gcgctcgccg aggccgggct gccggaggac
     2161 gcggtcgttt tgcacgccgg cggcggccac ggggagctag ccgagctggc gacccaggag
     2221 ggcctggtcg acctgctgat ccctcgcggg ggagagggcc tgaagaacgc gatcaaggac
     2281 gtggcgaccg tcccggtgat gtacgcggcg gcgggcaact gccacgtcta tgtgcacgcc
     2341 gaggccgacc cggagatggc gcggcagatc gcgctcaacg cgaaggtgca gcgccccggt
     2401 gtctgcaacg cggcggagac gctgctgctc gatgcggcga tcgccgacga gctcggtccg
     2461 gcgatcctcg gtgagctggc tgcggccggg gtcgagctgg tcggcgacga gcgcgcccgc
     2521 gggcttggcg gcgaggccga ggtcggcgtc gcgaccgacg aggactggga ccgcgagtac
     2581 ctggagctga agctggcggt cgcggtcgtc gactcggtcg aggaggcgat cgagcacgtg
     2641 aacgcgcacg gcaccgggca ctcggaggcg atcgtgaccg gatcggcgca ggcggcggac
     2701 gagtttgtcg ccggagtcga cgcggcggcg gtctacgtga acgcctcgac ccgcttcacc
     2761 gacggcttcg agtacgggat gggcgccgag atcggcaact cgacccagaa gctgcacgcc
     2821 cgcggcccga tcgggatgcg ggagctgacg actttcaagt acgtcgcccg cggcgacggc
     2881 cagattcgtg gctgacggcc gcaacggcgg catcggcgtc ctcggttcgg ccttcaaccc
     2941 gccgcacctg ggacacctgg cgctggcgca agtggcgctc tggcagctcg ggctcgacga
     3001 ggtcatcctc gtgccgaccg gcaccgcccc gcacaagcgg atcgccgacg accccggtcg
     3061 cgaggagcgg atggcgatga cccggctcgc cgccgccgat gactcgcgct tctcggtttc
     3121 cgccctggag gtcgaacgcg agggaccgtc ctacacgtat gagaccttgg ggctacttgc
     3181 cgaggaacga cccgaaaccg agctggtgtt cgtgatgggg gcggacgccg ccgtgggtct
     3241 ggagagctgg cgggaacccg agcgggtggt cggcctggcg cgcctggcag tcgcacgccg
     3301 c
//
LOCUS       DASRIG010000322         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2232653, whole genome shotgun sequence.
ACCESSION   DASRIG010000322 DASRIG010000000
VERSION     DASRIG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232653"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VFB52_08770"
     CDS             <1..1915
                     /locus_tag="VFB52_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06467.1"
                     /translation="KVLIKISNTAPAISGDGTRVVFLSNAALTTPNDDGNQEVYVALL
                     PRAAAAATFVRVTDTGKNFPDEFITEIFTNYQPAIGGDGTLVSFVSTRRVFNALENGT
                     AAFAAAAENAGSPSDPVTDGNAEIFLYSLGARAYTQVTLTRDQDATVNFVVKGFNSIP
                     QLSGNGRVLVFLSGFNYPGANANKNTDFNGEIFLYEVGAPTNTLTQVTDTTGIALVPV
                     PVLDQLGQPTGQFTVSLDAPMNLLTGFTRPLSRDGSLLTFESGGDFGGLNPGRTREVW
                     VYNTASKAFTRVTNQTASTIPTQLELQRIDYGFVPSLNAAGTYVTFVSTLNLTSGNAD
                     GSKEIYLYDVAGSTSESPKLRQLTTTQPSILFTDQRSNQYFSYPDNTGSRTTFATVSG
                     TIGANLSIANEIFQAVIRPVTETNAQAPSLANAASFDGAQVARGSIAAAFGTNLARGT
                     AQAASANLPFELGGVTVTVGGFAARLIFVAPGQINFVMPQGIADGDMIDFSVNNNGVQ
                     SAAKAKVVGASPGVFTATSDGTGAAAAQCGVVMNLSFVITAPPCAASTESEQRFLILY
                     GTGWRLSGAANVTLRVGDQALTVNYAGPQGDFPGLDQINAVIPAALMGKGEQDTVVTA
                     SSQASKTVKVTIQ"
     gene            complement(1944..2213)
                     /locus_tag="VFB52_08775"
     CDS             complement(1944..2213)
                     /locus_tag="VFB52_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06468.1"
                     /translation="MDQAEKVRAELGRYEHPLFDFRVEAAAGGVAVTVLLKPEVGAVA
                     HDYRFLVADRELASPHFAWDFQRQLFNYLHDYLVEMFTRTPQTLP"
BASE COUNT          430 a          842 c          782 g          368 t
ORIGIN      
        1 caaggtgctc atcaagatct cgaacacggc gccggcgatc agcggcgacg ggacgcgcgt
       61 cgtcttcctc tcaaacgccg cgctgacgac gccgaacgac gacggcaacc aggaggtcta
      121 cgtcgcgctc ctgccgcgtg ccgccgcggc ggcgaccttc gtccgcgtga ccgacaccgg
      181 gaagaacttc cccgacgaat tcatcacgga gatcttcacc aactaccagc cggcgatcgg
      241 cggcgacggg acgctggtca gcttcgtctc gacgcgccgg gtcttcaacg cgctcgagaa
      301 cggcacggcc gccttcgccg ccgccgccga gaacgccggc agcccgagcg acccggtgac
      361 cgacggcaac gcggagatct tcctctacag cctcggcgcc cgcgcctata cacaggtgac
      421 gctcacgcgc gaccaagacg cgacggtcaa cttcgtcgtc aagggtttca acagcatccc
      481 gcagctgagc ggcaacgggc gggtgctcgt tttcctctcg ggctttaact accccggggc
      541 gaacgcgaac aagaacacgg atttcaacgg cgagatcttc ctctacgagg tgggggcgcc
      601 gaccaacacc ctgacgcagg tgaccgacac gaccggcatc gccctcgtcc cggtcccggt
      661 cctggaccag ctcggccagc cgaccggcca attcactgtc tccctggacg cgccgatgaa
      721 tctcttgacc gggttcaccc gcccgctgag ccgcgacggc agcctgctga ccttcgagtc
      781 ggggggcgac ttcggcggcc tcaacccggg acggacgcgg gaggtctggg tttacaacac
      841 cgcctcgaaa gcctttaccc gcgtcactaa ccagaccgcc tcgaccatcc cgacgcagtt
      901 ggagctgcag cggatcgatt acgggttcgt cccgagcctt aacgccgccg ggacctacgt
      961 cactttcgtc tcgacgctca acctgacgag cggcaacgcg gacgggagca aggagatcta
     1021 tttatacgac gtcgccggct cgacctcgga gagcccgaag ctgcgccagc tgacgacgac
     1081 gcagccctcg atcctcttca ccgaccagcg ctcgaaccag tacttttcct acccggacaa
     1141 caccggcagc cggacgacct tcgccaccgt ctcggggacc atcggggcga atctgagcat
     1201 cgccaacgag attttccagg ccgtcatccg gccggtgacc gagacgaacg cgcaggcccc
     1261 gtcgctggcc aacgcggcga gcttcgacgg ggcgcaggtc gcgcgcggct cgatcgccgc
     1321 ggccttcggc accaacctcg cccgcgggac cgcccaggcg gcgagcgcca acctgccctt
     1381 cgagctcggc ggcgtgaccg tgaccgtcgg cggcttcgcc gcgcgcctca tcttcgtcgc
     1441 gcccgggcag atcaacttcg tcatgcccca gggcatcgcc gacggcgaca tgatcgactt
     1501 ctcggtcaac aacaacggcg tccagtcggc ggccaaggcc aaggtcgtcg gcgcctcgcc
     1561 gggggtcttc accgcgacga gcgacgggac gggcgcggcg gcggcgcagt gcggcgtggt
     1621 catgaacctg agcttcgtca tcaccgcccc gccgtgcgcg gcgagcaccg agtcggagca
     1681 gaggttcctc atcctttacg ggacgggctg gcggctctcg ggggcggcga acgtgacctt
     1741 gagggtcgga gatcaggcgc tcacggtgaa ttacgccggc ccccagggcg acttcccggg
     1801 cctcgaccag atcaacgccg tcatcccggc ggccctcatg ggcaagggcg agcaggacac
     1861 agtcgtcacc gccagctctc aggcgagcaa gaccgtcaag gtgacgatcc agtagtctca
     1921 aacctcaggg ttcagattcg aaattacgga agcgtctgcg gcgtgcgcgt gaacatctcg
     1981 acgagatagt cgtggaggta gttgaagagc tggcgctgga agtcccaggc gaagtggggg
     2041 ctcgcaagct cgcggtcggc aaccaggaag cggtagtcgt gggcgaccgc cccgacctcg
     2101 ggtttgagta agacggtaac cgctacgccc cccgcggcgg cctcgacccg gaagtcgaag
     2161 agcgggtgct cgtagcgccc gagctcggcg cgaactttct cggcttggtc catgattgta
     2221 gggggatcgt aggggcaggg ctggtctctg cccgcggtcg cgatggcgaa ccgcgggcag
     2281 ggaccagccc tgcccctaca tctctattta gagcagctta actcgtttga gccggagcgc
     2341 gatccggcgc tggccgcgct ggagcgtgag gtcgacctcg gaattttcgg ggccgcggag
     2401 tgcgtcgacc agcccgccga ac
//
LOCUS       DASRIG010000323         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_690632, whole genome shotgun sequence.
ACCESSION   DASRIG010000323 DASRIG010000000
VERSION     DASRIG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690632"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFB52_08780"
     CDS             <1..206
                     /locus_tag="VFB52_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06469.1"
                     /translation="IEKGLERLLQGRTAIVIAHRLSTIRRAGKIVVLADGGIAEQGTH
                     DELIAAGGPYSKLYGAWADSSAA"
     gene            complement(443..1129)
                     /locus_tag="VFB52_08785"
     CDS             complement(443..1129)
                     /locus_tag="VFB52_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06470.1"
                     /translation="MVALIALVAAGCGGGGDDTTAPTAETTVEEPTAISKAELISRGD
                     GICGEVNAAIGAIGASEEGEVDEQTTEVADLYIGLVESIKDLGTPTETEGYEEFIGAA
                     DDLAAIEGEIKLAAERSDVEALGEAGTEATAALEEFQAQAAIYGFEECSEPPSAPTIT
                     PGTGGAEGGEIEEGGVEVTPEIEEEVVPEEIVPEEEVTPEGGGVGGEEEVAPETGGEE
                     GSSGGGIGPG"
     gene            complement(1201..1271)
                     /locus_tag="VFB52_08790"
     tRNA            complement(1201..1271)
                     /locus_tag="VFB52_08790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1237..1239),aa:Gln,seq:ctg)
     gene            complement(1292..2485)
                     /locus_tag="VFB52_08795"
     CDS             complement(1292..2485)
                     /locus_tag="VFB52_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO06471.1"
                     /translation="MPDREVDFLLIGGGMASASCASELRKRGSEGSVLLVGREPEPPY
                     ERPPVSKEYLRGEAERSDAYVNQPAWYEENGVELLTGKNVMGLDTEARVAKIQGGEEV
                     AFGKALIATGAMVNILRVEGAENEGIHYLRAFGNSDAIRADAEKAEHVVLIGGSYIGT
                     EVAASLTAHGTKCSIIAMEDVALSRTFGTEAGRWFQDLIEAKGVEFHGGEELEAFEGD
                     GRISAVVTKSGLSIECDAVVVGAGVRPDAMLAQRAGIEVDGAVVCDSKLQTSVPGIYA
                     AGDCCSYDSVVHGRRIHVEHWDVSMHQGMHAARNMLGEDADYDVVPYFFSDLADWASL
                     EYVGPAEDWDEEVWRGDREAGEFSVWYLKDGKVAGCLSVERSEELARAREMIAEGTEF
                     SEAAL"
     gene            complement(2478..3158)
                     /locus_tag="VFB52_08800"
     CDS             complement(2478..3158)
                     /locus_tag="VFB52_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZO06472.1"
                     /translation="MYIIVVGAGKVGWNLARELLEKEHEVTLIEADRRRYLTVEQELE
                     HNISYGDASELWVLERAGIQRADMVIAVTGDDEDNMLICQVAKEKYLVEQIIARVNNP
                     RNREHFDLLGVKPSVSATDLILRLLEHEVPEYGLVHLLDLQEEQLEIIEMLLGKDSTV
                     TGRRVGDLQMPDGSLLISVLRAGKGFVPTADTVLEEGDEILAVLDPGKEEDLKALFGP
                     ESNSGNGA"
     gene            complement(3174..3620)
                     /locus_tag="VFB52_08805"
     CDS             complement(3174..3620)
                     /locus_tag="VFB52_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZO06473.1"
                     /translation="MFILIVGCGRVGSSVARAMLHEGHEVSCLDEDPESHARLEVGLE
                     KSWEDLGGQFTVGAGLEIEALVAAGIEQADIFISSTDGDNTNIIIAQIAQRRYEVPNV
                     IARVLDPLRAEWYQRQGLNTVCPTRVAIDMLEETVHQASGRAAGRA"
     gene            complement(3631..5643)
                     /locus_tag="VFB52_08810"
     CDS             complement(3631..5643)
                     /locus_tag="VFB52_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO06474.1"
                     /translation="MSGPPENDAPAGKPGRRQQLAQRSRRRIERGTGVPWLFAAAYSA
                     VGFSIYFALGVVADRGLGLTPLIFLAAGLLFGLTTLTYVEGGAMFRERGGSSTFARHA
                     FNELIAFIAGWAILIDYLIVLALAAISVPHYLAPISADFGKPGLEIPIAALVIAAVCA
                     LNILNVTGRARQRSLALLALADVLLQLAVIVVGVLVVMHPERLTAELDLFTSPSLRDT
                     VYAFVVAMLAYAGIEAASDLAPDIEVSRRDLKRVASLGAVAVPLVYAGMAAIALMAVP
                     VVAGPGGPETALGNQFVEEPVLGVVSAFEPGWVADSMRWMVMLVAVPVLFWAANTSML
                     GVSRHIYTLAINRQIPSWLGKLEKRKATPYVAIVICGVIALGLVIPTNVKLLAGIYAF
                     GATLAITIAHLSIIRLRIGKPDKQRPFKIPWNVNWRGAELPLPALFAAVVSGLAFLSV
                     LAYHSTARWVGLGWMAFGLLFYVVYRKVFEGTTLTKRVSVSERALTKQMPAVEYGNIL
                     VPVFGTKFDDDIVGTAGRLASAEQKQEDGSDPRLDVIYVIEVPLTLPLDADLPEEMEA
                     EAQKALERAREVGEEYEDVEVSTQVIRARKVGAGIVEAARESGAEAIVIGGEEPTKIR
                     GGATIGGIGAAKPAEIGAATEYVLKKAPCRVLLTAPPETSASRDSA"
     gene            complement(5640..5843)
                     /locus_tag="VFB52_08815"
     CDS             complement(5640..5843)
                     /locus_tag="VFB52_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06475.1"
                     /translation="MSGPGGGERLYRGAVRGFSVVFILIGLVVLVTTLANGGSPASVG
                     FFMGLAFVAVGALRLWVATRTGS"
     gene            complement(5840..7738)
                     /locus_tag="VFB52_08820"
     CDS             complement(5840..7738)
                     /locus_tag="VFB52_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZO06476.1"
                     /translation="MAQRIPRDESLSRVHGVGALFSAAYGNVGSSIYYALGVTAAFAL
                     GLTPVAFVISGLIFAATAATYAEATVMYPEAGGSSSFARHAFNEFVSFVAAWGQMLNY
                     TITVAISAFFIPHYLAVFWPWLGSSPGDIIGGAAVIVALALINIRGTEESAKLNLVLA
                     VADLATQVVLVLIGLFLVFNPEVLIDNVHLGVAPTWSDFALGIAVGMIAYTGIETISN
                     MAEEARDAARTIPRSVGLTVVAVLGLYLLIPIVALSAMPVTQNAAGEYSTTLGTTFAD
                     DPILGIVENLGLAHGMTEVLRYYVGVLAAVILLIATNAGLIGVSRLTYSMGQHRQLPE
                     GLRQVHPKYRTPYIAIMIFAAVAIVTLLPGQTDFLATLYSFGAMLSFTIAHVAVIQLR
                     RKHGDADRSWKPPLNFRAFGFEVPLTAVLGGLGTFAAWLVVMALNPRTLFVGLGWMAL
                     GIVIYVLYRRNQRLPLTETVKVVLPEPLGVEEVEYKSVLVGFEDDEPFNGEMVATAVK
                     LASKRRRGIHIHSMMTVPTHLPLNAELPEQEAEAQAKVEEAKLIGGTRVTGHVARVRP
                     GQAGYSISEEAEMIQAAAIVIGLRYRNGVPLYDKTLQTVLGERPCRVIVVSDPTEQLT
                     TVAAGALS"
     gene            complement(7774..>8128)
                     /locus_tag="VFB52_08825"
     CDS             complement(7774..>8128)
                     /locus_tag="VFB52_08825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZO06477.1"
                     /translation="VVSNHGGRQLDRSPATGDVLTEIVDAVDGRVPVVVDGGIRRGID
                     MAIALALGADAVQVGSPALWGLAGGGEAGVARVLAMLRAEFELALSLCGCRTPGELTR
                     AHVQRAPATGVYSRQ"
BASE COUNT         1275 a         2804 c         2796 g         1253 t
ORIGIN      
        1 cgatcgagaa aggcctcgag cgcctgctcc agggccgcac cgcgatcgtc atcgcccacc
       61 gcctctcgac catccgccgc gccggcaaga tcgtcgtcct cgcggacggg ggcattgccg
      121 agcagggtac ccacgatgaa ctgatcgcgg caggcggtcc ctactcaaag ctctacggcg
      181 cctgggccga ctcgtcagcc gcctgagcgc cagggcggaa gctgcgacac tgccgattcg
      241 ccgggcacgg tgtcggcccg atccttcggg gagctgtgat ggagggctgt caatcccctc
      301 cccgggaatg tgccgaaggc cccaatcttc gatctgatca agggcccgcc ataggcgggc
      361 tcaatctggg ggagccaccc caaagggggg cgactgaatt cacggggagg ggattgacag
      421 ccctcccccg caacgccccg aatcagcccg ggccgatgcc gccgccggag gagccttctt
      481 cccctccggt ttcgggagcc acttcttctt cgcccccaac acccccacct tcaggcgtca
      541 cttcttcttc cgggacgatt tcttccggca cgacttcttc ttcgatttcg ggggtgactt
      601 cgacgccgcc ttcttcgatt tcgccacctt cggcaccgcc ggtgccgggc gtgatggtcg
      661 gggcgctcgg gggttcggag cactcttcga agccgtagat cgcggcctgg gcctggaatt
      721 cttcgagggc cgcggtcgct tcggtgccgg cttcgccgag cgcttcgacg tcgctgcgtt
      781 cggcggcgag tttgatttcg ccctcgatcg cggcaaggtc gtcggcggcg ccgatgaatt
      841 cttcgtagcc ctcagtttcg gtcggggtac cgaggtcttt gatgctttcc accaagccga
      901 tgtagaggtc ggcgacttcg gtggtctgtt cgtcgacttc gccttcttcg gaggcgccga
      961 tcgcgccgat cgcggcgttc acctcgccgc agatcccgtc gcccctggag atcagttcgg
     1021 ctttcgagat cgccgtcggt tcttcgaccg tcgtttcggc ggtcggcgcg gtcgtgtcgt
     1081 caccgccacc gccgcagccg gcggcgacca gcgcaatcaa cgcgaccaac gcgaacagcg
     1141 agataggtac tcgcaagagc ctcattccga ggcgaagcta gcaaaggaga tagacaccta
     1201 ctgcgcccca gggactcgaa ccccgactac ctcgtccaga gcgaggcgtg ctgccattac
     1261 accagggcgc aacgaagctt ccgcaggata cctagagagc ggcctcggag aactcagtcc
     1321 cctcggcgat catctctctt gcacgcgcca gttcttcgga gcgctcgacg ctgaggcagc
     1381 cggcgacctt gccgtccttc aggtaccaga ccgagaactc gccggcctca cggtcgccgc
     1441 gccagacctc ctcgtcccag tcctcggccg ggccgacgta ctcgaggctg gcccagtcgg
     1501 cgagatcgct gaagaagtag gggacgacgt cgtagtcggc gtcctcaccg agcatgttgc
     1561 gggccgcgtg catgccctgg tgcatcgaga cgtcccagtg ctcgacgtgg atgcggcggc
     1621 cgtgcacgac gctgtcgtag gagcagcaat cgcccgccgc gtagatgccc ggcaccgagg
     1681 tctgcagctt cgagtcgcag acgaccgcgc cgtcgacctc gatcccggcc cgctgggcca
     1741 gcatcgcgtc gggccgcacg ccggcgccga cgacgactgc gtcgcactcg atcgagagcc
     1801 cgctcttggt caccaccgcg gagatccgcc cgtcgccctc gaaagcctcc agctcctcgc
     1861 cgccgtggaa ctcgacgccc ttcgcctcga tcaggtcctg gaaccaacgc ccggcctcgg
     1921 tgccgaaagt gcgcgacaag gccacgtcct ccatggcgat gatcgagcac ttggtgccgt
     1981 gcgcggtcag cgaggcggcg acctcggtgc cgatgtagct gccgccgatc aggaccacgt
     2041 gctcggcctt ctcggcgtcg gcccggatcg cgtccgagtt gccgaacgcg cgcaggtagt
     2101 ggatgccctc gttctcggcg ccctcgacgc ggaggatgtt gaccatcgcc ccggtggcga
     2161 tcagcgcctt gccgaaggcg acctcttccc cgccctggat cttcgccacc cgcgcctcgg
     2221 tgtcgagccc catcacgttc ttgccggtca gcagctcgac cccgttctcc tcgtaccagg
     2281 cgggctggtt gacgtaggcg tcgctgcgct cggcctcgcc gcgcaggtac tccttggaga
     2341 ccggcgggcg ttcgtagggc ggctcgggct cgcgaccgac caacagcacc gagccctcgc
     2401 tgccacgctt gcgcagctcg gaggcgcagc tggccgaggc catgccgccg ccgatcagca
     2461 ggaagtcgac ctcgcggtca ggcaccgttg ccgctgttgc tttccggtcc gaagagggcc
     2521 ttcaagtcct cctctttacc cggatccagc accgccaaaa tctcgtcgcc ttcctcgagc
     2581 acggtgtcgg cggtgggcac gaagcccttg ccggcgcgca gcaccgagat cagcaggctg
     2641 ccgtcgggca tctgcaggtc gccgacccgg cggccggtca cggtcgagtc cttgccgagc
     2701 agcatctcga tgatctccag ctgctcctcc tgcaggtcga gcaggtggac gagcccgtac
     2761 tcgggcacct cgtgctcgag caggcgcagg atcaggtcgg tggcggaaac gctcggtttg
     2821 accccgagca ggtcgaagtg ctcgcggttg cgggggttgt tgacccgggc gatgatctgc
     2881 tcgaccaggt acttctcttt cgccacctgg cagatcagca tgttgtcctc gtcatcgccg
     2941 gtcaccgcga tcaccatgtc ggcgcgctgg atcccggccc gctccagcac ccagagctcg
     3001 gaggcgtcgc cgtaggagat gttgtgctca agctcctgct cgacggttag gtagcggcgc
     3061 cggtcggcct cgatcaaggt cacctcatgt tccttctcga gcagctcgcg agccaggttc
     3121 catcccacct tgcccgcgcc gacgacgatg atgtacatgc gccccaagtc ctcctacgcg
     3181 cgccccgcgg cgcggccgga cgcctggtgc accgtctcct cgagcatgtc gatcgcgacc
     3241 cgggtcgggc agaccgtgtt caagccctgg cgctggtacc actcggctcg cagcgggtcg
     3301 agcacccgcg cgatcacgtt cggcacctca taccggcgct gggcgatctg ggcgatgatg
     3361 atgttcgtgt tgtcgccgtc ggtcgaggag atgaagatgt ccgcctgctc gatcccggcc
     3421 gcgaccagcg cctcgatctc gagcccggcg ccgaccgtga actgaccgcc gaggtcctcc
     3481 cagctcttct ccaggccgac ctcgagccgg gcgtgggact cggggtcctc gtcgaggcag
     3541 gagacttcgt ggccctcgtg cagcatcgcg cgcgccaccg aagagccgac ccgcccgcat
     3601 ccgacgatca agatgaacac aggccggata ctaggcgcta tcccggctgg cggaggtctc
     3661 cggcggcgcg gttagtaaca cgcggcacgg agccttcttg agcacgtact cggtggcggc
     3721 accgatctcg gccggcttgg ccgcgccgat cccgccgatc gtcgccccgc cgcggatctt
     3781 ggtcggctcc tcgccgccga tcacgatcgc ctcggcgccc gactcgcggg ccgcttcgac
     3841 gatcccggcg ccgaccttgc gggcgcggat cacctgcgtc gagacctcga cgtcctcgta
     3901 ctcctcgccg acctcgcgcg cccgctccag cgccttctgc gcctctgcct ccatctcctc
     3961 cggcaggtcg gcgtcgagcg gcagcgtgag cggcacttcg atcacgtaga tcacgtcgag
     4021 gcgcgggtcc gagccgtcct cctgcttctg ctcggctgac gccagccggc ccgccgtgcc
     4081 gacgatgtcg tcgtcgaact tggtcccgaa gacgggcacg aggatgttgc cgtattcgac
     4141 cgcgggcatc tgcttggtca gcgcccgctc ggagaccgag acgcgcttgg tcagcgtcgt
     4201 gccctcgaag accttgcggt agacgacgta gaagagcagc ccgaaggcca tccagccgag
     4261 cccgacccag cgcgcggtcg agtggtaggc gagcacgctg aggaaggcga ggccgctgac
     4321 cacggcggcg aacagggccg gcaggggcag ctcggcaccg cgccaattga cgttccaggg
     4381 gatcttgaac gggcgctgct tgtccggctt gccgatccgc aggcggatga tcgagagatg
     4441 ggcgatcgtg atcgccaggg tcgcgccgaa ggcgtagatc ccagccaaga gcttgacgtt
     4501 ggtcgggatc accagcccga gcgcgatcac gccgcagatc acgatcgcca cgtagggcgt
     4561 cgccttgcgc ttctcgagct tgccgagcca gctggggatc tggcggttga tcgcgagcgt
     4621 gtagatgtgg cgggagaccc cgagcatcga ggtgttggcg gcccagaaga ggaccgggac
     4681 cgcgaccagc atcaccatcc agcgcatcga gtcggcgacc cacccgggtt cgaaggccga
     4741 gaccactccc agcaccggct cctcgacgaa ctggttgccg agcgcggtct ccggcccgcc
     4801 cggcccggcg acgaccggca ccgccatcag cgcgatcgcc gccatcccgg cgtagacgag
     4861 cggcacggcg accgccccca acgaggcgac ccgcttgagg tcgcggcggc tgacttcgat
     4921 gtcgggcgcc agatccgagg cggcctcgat ccccgcgtag gcgagcatcg cgaccacgaa
     4981 ggcgtagacg gtgtcgcgca ggctcgggct ggtgaacagg tcgagctcgg cggtcagccg
     5041 ctcggggtgc atgacgacga gcaccccgac gacgatcacc gccagctgca ggagcacgtc
     5101 cgcgagcgcc agcagggcca gcgagcgctg ccgcgcgcgg ccggtgacgt tgaggatgtt
     5161 gagcgcgcag accgcggcga tcaccagcgc cgcgatcggg atctccagcc ccggcttgcc
     5221 gaagtcggcg gagatcgggg ccaggtagtg aggcaccgag atcgccgcca gggcgagcac
     5281 gatcaggtag tcgatcagga tcgcccagcc ggcgatgaag gcgatcagct cgttgaaggc
     5341 gtggcgggcg aaggtcgagg agccgccgcg ctcgcggaac atcgcgccgc cctcgacgta
     5401 ggtgagcgtg gtcaggccga agagcaggcc ggcggcgagg aagatcagcg gggtgaggcc
     5461 gaggccgcgg tcggcgacga cgccgagcgc gaagtagatc gagaagccga ccgccgagta
     5521 ggcggccgcg aacagccagg gcacgccggt accgcgctcg atccgccgcc ggctgcgctg
     5581 ggcgagctgc tggcggcgac cgggcttgcc ggcgggcgcg tcgttctccg gagggccgct
     5641 cacgatcccg tcctggtcgc gacccagagg cgcagggcgc cgaccgcgac gaaggcgagg
     5701 cccatgaaga agccgaccga ggcggggctg ccgccgttgg ccagggtggt cacgagcaca
     5761 accaggccga tcaggatgaa gacgaccgag aagccgcgca cggcgccgcg gtagagccgc
     5821 tctcccccac ccggcccgct cacgagagcg cccccgccgc gaccgtggtc agctgttcgg
     5881 tcggatcgga gacgacgatc acccggcagg gccgctcgcc gagcaccgtc tgcagggtct
     5941 tgtcgtagag cggcaccccg ttgcggtagc gcaggccgat cacgatcgcc gccgcctgga
     6001 tcatctccgc ctcctcggaa atcgagtagc cggcctggcc cgggcgcacg cgggcgacgt
     6061 gaccggtcac ccgggtgccg ccgatcagct tcgcctcctc gaccttggcc tgggcttcgg
     6121 cttcctgttc gggcagctcg gcgttcagcg gcaggtgcgt cggcacggtc atcatcgagt
     6181 ggatgtggat gccgcggcgg cgtttggagg cgagcttgac cgcggtcgcg accatctcgc
     6241 cgttgaacgg ctcgtcgtcc tcgaagccga ccaggaccga tttgtactcg acctcttcga
     6301 ccccgagcgg ctccggcagc accaccttca ccgtttcggt cagcggcagc cgctggttgc
     6361 ggcggtagag cacgtagatg acgatcccga gcgccatcca gccgagcccg acaaacagcg
     6421 tgcgtgggtt gagcgccatg accacgagcc aggcggcgaa ggtgccgagg ccgccgagca
     6481 ccgcggtcag cggcacctca aaaccgaagg cgcggaagtt gagcggcggc ttccaggaac
     6541 gatcggcgtc gccgtgtttt cgccgcagct ggatcaccgc gacgtgggcg atcgtgaacg
     6601 agagcatcgc gccgaacgag tacagcgtcg ccaggaagtc ggtctggccc ggcagcaggg
     6661 tgacgatcgc gaccgcggcg aagatcatga tcgcgatgta gggggtgcgg tacttcgggt
     6721 ggacctggcg cagtccctcg ggcagttggc ggtgctggcc catcgagtag gtcagccggg
     6781 agacgccgat cagccccgcg ttggtcgcga tcagcaggat caccgccgcc agcacgccga
     6841 cgtagtagcg gaggacttcg gtcatcccgt gggcgagtcc caggttctcg acgatgccga
     6901 ggatcgggtc gtcggcgaag gtcgtgccca gcgtggtcga gtattcgccg gccgcgttct
     6961 gggtcaccgg catcgccgag agcgcgacga tcgggatcag caggtagagg ccgagcacgg
     7021 cgacgacggt gaggccgacg ctgcgcggaa tcgtgcgcgc cgcgtcgcgc gcctcctcgg
     7081 ccatgttcga gatcgtctcg atcccggtgt aggcgatcat cccgaccgcg atcccgagcg
     7141 cgaagtcact ccaggtcggg gcgaccccga ggtggacgtt gtcgatcagg acctccgggt
     7201 tgaagacgag gaagagcccg atcaggacca gcacgacctg ggtcgcgagg tcggcgaccg
     7261 cgagaactag attgagtttc gcggactcct cggtgccgcg gatgttgatc agcgcgagcg
     7321 cgacgatcac cgccgcgccg ccgatgatgt ccccggggga gctccccagc cagggccaga
     7381 agaccgccag gtagtggggg atgaagaagg ccgagatcgc gaccgtgatc gtgtagttga
     7441 gcatctgccc ccaggccgcg acgaagctca cgaactcgtt gaaggcgtgg cgggcgaagc
     7501 tggaggagcc gccggcctcc gggtacatca cggtcgcctc ggcgtaggtg gcggcggtgg
     7561 cggcgaagat cagcccggag atcacgaagg cgaccggggt gaggccgagg gcgaaggcgg
     7621 cggtgacgcc gagcgcgtag tagatcgagg agccgacgtt gccgtaggcg gccgagaaca
     7681 gcgcgccgac gccgtggacg cggctcagcg actcatctct ggggatgcgc tgggccatct
     7741 atgtgccgcg agggaaggtc ctgccggggc gagtcactgg cgcgaatata cgccggtcgc
     7801 cggtgcgcgc tgcacgtggg cgcgtgtgag ctcgcccggc gtcctgcagc cgcagaggga
     7861 gagcgccagc tcgaactcgg cgcggagcat cgcgagcacc cgggcgacac cggcctcgcc
     7921 gccgccggcg agcccccaga gtgccgggct gccgacctgc acggcgtcgg cgccgagcgc
     7981 cagcgcgatc gccatgtcga tcccgcggcg gatgccgccg tcgaccacca ccgggacgcg
     8041 gccgtcgacc gcgtcgacga tctcggtgag cacgtcgccg gtcgccggcg agcggtcgag
     8101 ctggcgcccg ccatggttgg agacgacg
//
LOCUS       DASRIG010000324         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_691750, whole genome shotgun sequence.
ACCESSION   DASRIG010000324 DASRIG010000000
VERSION     DASRIG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691750"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="truA"
                     /locus_tag="VFB52_08830"
     CDS             <1..721
                     /gene="truA"
                     /locus_tag="VFB52_08830"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZO06478.1"
                     /translation="LDIEYDGSGFRGWARQPGLRTVQGELETALATVLREAIDLTVAG
                     RTDTGVHARGQVASFDTPAEPPADLARSLNGLGPDDIGVTAAAVVADGFDARRDARSR
                     TYRYRLLARRAPSPFEQGRALWWPHRVDRDALDACAAALPGTHDFTAFTPTQTDHVRF
                     DRDILAVAWSQTGDVLEFEITADAFMRNMVRALVGTQLEVASGRRAVENFSALLEGAP
                     RSAGGDTAPPHGLYLESVSYD"
     gene            complement(818..1171)
                     /gene="rplQ"
                     /locus_tag="VFB52_08835"
     CDS             complement(818..1171)
                     /gene="rplQ"
                     /locus_tag="VFB52_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZO06479.1"
                     /translation="MRHKKKRNKLSRSSAHREALLANLSKQLIEHERIETSQAKAKAV
                     KPEVEQLITLAKRGDLHARRQALSALHNDKFIVHKLFDEVAPRYETRPGGYTRIVKLG
                     PRRSDSTEMVYLELV"
     gene            complement(1197..2177)
                     /locus_tag="VFB52_08840"
     CDS             complement(1197..2177)
                     /locus_tag="VFB52_08840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZO06480.1"
                     /translation="MTTDFQVPRIISEKVDDRRGTFTIEPLDKGFGYTFGNSLRRVLL
                     SSLGGAAITSVRIEGVAHEFSTVPGVKEDVTDIVLNLKDLVVRMHTDADEVEAPLVAT
                     GPGEVKAKDIDLPSGVEILNPDAPIATLEKKTKLEVYLTIGRGRGYSPAEENKSDEQP
                     IGVIPIDSIFSPIRRASYAVSAARVGQRTDFDKLELDLETDGSIEPAAALREASEILI
                     KSLAIFTDAERVEELTAREPLPTPLSDPLSGGSGDGVAANNGLDEILIEELELGVRSY
                     NCLKRAGVQTVGDLVRKSRAELNAIPNFGQKSIEEVIETLHARGLDLQQD"
     gene            complement(2217..>2651)
                     /gene="rpsD"
                     /locus_tag="VFB52_08845"
     CDS             complement(2217..>2651)
                     /gene="rpsD"
                     /locus_tag="VFB52_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZO06481.1"
                     /translation="TRRYYQVLEKQFRAYYDKASRQDGVTGENLLRLLESRFDNVLVR
                     LGFAASRRQARQLISHGHWMINGRRVDIPSYQVRPEDIISIKAGSSATDTIQRATELV
                     STVPAWLQADHDALTAKVLRYPERSEISAPVQEQLIVELYSK"
BASE COUNT          389 a          905 c          898 g          459 t
ORIGIN      
        1 gctcgacatc gaatacgacg gatccggctt ccgcggctgg gcccgccagc ccggcctacg
       61 caccgtgcaa ggcgagctgg agaccgccct ggcaaccgtt ctgcgcgaag cgatcgacct
      121 gaccgttgcc ggccgcaccg acaccggcgt gcacgcgcgc ggccaggtcg cgagcttcga
      181 cacgcccgcc gagccccccg ccgacctcgc ccgcagcctc aacggcctcg gccccgacga
      241 catcggcgtg accgccgccg cggtcgtggc cgacggcttt gacgcccgcc gcgacgcccg
      301 ctcgcgcacc taccgctacc gcctgctcgc ccgccgcgcc cccagcccct tcgagcaggg
      361 ccgcgcgctc tggtggcccc accgggtcga ccgcgacgcg ctcgacgcct gcgccgccgc
      421 cctccccggc acccacgact tcaccgcctt caccccgacc cagaccgacc acgtccgctt
      481 cgaccgcgac atcctcgccg tcgcctggag ccagacgggc gacgtgctcg aattcgagat
      541 caccgccgac gccttcatgc gcaacatggt ccgcgccctg gtcggcacgc agctagaagt
      601 cgcctccggc cgccgcgcgg tcgagaactt ctcggcgcta ctcgagggcg ccccccggtc
      661 ggcaggaggc gatacggccc caccccacgg cctttacctc gagtcggtct cctacgattg
      721 atgggtgggc cggggaggcc ctcccaggac actcaacatc gagccatagg cgagatgtgt
      781 ccggggaggg cctccccggc ccaccccttc gccctagcta gaccagctcg aggtagacca
      841 tctcggtgga gtcgctccgc cgagggccca gcttgacgat gcgggtgtag ccgccgggcc
      901 gcgtctcgta ccgcggcgcc acctcgtcga acagcttgtg gacgatgaac ttgtcgttgt
      961 gaagagccga gagcgcctgg cggcgagcgt gcaggtcgcc ccgtttggcg agcgtgatca
     1021 gctgctcgac ctccggcttg accgccttcg ccttcgcctg gctggtttcg atccgctcgt
     1081 gctcgatcaa ctgcttggag aggttggcga gcagcgcctc acggtgggcg gagctacggc
     1141 tcagcttgtt ccgcttcttt ttgtggcgca tcggtctctc ccctagtctc tcgcgcttag
     1201 tcctgctgca ggtcgaggcc gcgcgcgtgc agggtctcga tcacttcctc gatcgacttc
     1261 tggccgaagt tcgggatcgc gttgagttcc gcgcgcgact tgcgcacgag gtcgccgacg
     1321 gtctgcacgc cggcgcgctt caggcagttg tacgaacgga cgccgagttc gagctcctcg
     1381 atcaggatct cgtcgaggcc gttgttggcc gcaaccccgt cgccggagcc gcccgagagc
     1441 ggatcgctga gcggcgtcgg cagcggttca cgagcggtca gttcctcgac ccgctcggcg
     1501 tcggtgaaga tcgccaggct cttgatcagg atctcggagg cctcgcgcag cgccgccgcg
     1561 ggctcgatcg agccgtcggt ctcgaggtcg agctcgagtt tgtcgaagtc ggtgcgctgg
     1621 ccgacgcggg cggcggagac cgcgtaggaa gcgcggcgga tcggcgagaa gatcgagtcg
     1681 atcgggatca cgccgatcgg ctgctcgtcg gacttgttct cttccgccgg cgagtagccg
     1741 cggccgcggc cgatcgtcag gtagacctcg agtttggttt ttttctcgag cgtcgcgatc
     1801 ggcgcgtcgg ggttaaggat ctcgacgccg gaaggcaggt cgatgtcctt cgctttcacc
     1861 tcgccggggc cggtcgcgac cagcggggcc tcgacctcgt cggcgtcggt gtgcatgcgg
     1921 acgaccaggt ccttgaggtt gaggacgatg tcggtgacgt cctctttgac gcccgggacg
     1981 gtcgagaact cgtgcgcgac gccctcgata cggacgctgg tgatcgcggc gccacccagc
     2041 gaggagagca gcacgcggcg caggctgttg ccgaaggtgt agccgaagcc cttgtcgagc
     2101 ggctcgatcg tgaacgtgcc gcgacggtcg tcgactttct cggagatgat gcgggggacc
     2161 tggaaatcag tggtcatgag ttccttcgta tgcgggttgc gaaatcggtt tccggactac
     2221 ttggagtaca gctcgacgat cagctgctcc tgaaccggcg ccgagatctc ggagcgctcg
     2281 gggtagcgca ggaccttggc ggtgagggca tcgtggtcgg cctgcagcca ggcgggaacc
     2341 gtcgaaacga gttcggtggc acgctggatc gtgtccgtgg ctgaggagcc ggccttgatc
     2401 gagatgatgt cctcgggacg cacctgatag gacgggatgt cgacgcggcg gccgttgatc
     2461 atccagtggc cgtgactgat caactggcgg gcctgacgcc gcgaggcggc gaagccgagc
     2521 cgcacgagca cgttgtcaaa acgcgactcg agcagacgca gcaggttctc accggtgacg
     2581 ccgtcctggc gcgaagcttt gtcgtagtag gcgcggaact gtttctccag cacctggtaa
     2641 tagcggcgcg t
//
LOCUS       DASRIG010000325         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3273324, whole genome shotgun sequence.
ACCESSION   DASRIG010000325 DASRIG010000000
VERSION     DASRIG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273324"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFB52_08850"
     CDS             complement(<1..300)
                     /locus_tag="VFB52_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZO06482.1"
                     /translation="MDEPLAIAVDALIVTDDQGRVIEWNESAVQVFGFKREEVLGRHL
                     SRLFASPPGEARPRSEAESQSWRKRIRAALSEERLLLAAQPVVDLRSGNVDRHELL"
     gene            455..1732
                     /locus_tag="VFB52_08855"
     CDS             455..1732
                     /locus_tag="VFB52_08855"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZO06483.1"
                     /translation="MTDEQTVQKLQSFSRDQGSERVVHAKGSAAHGFFEATEDVSQFT
                     KAGLFRKGARTPLFLRFSTEAGEHGSADTARDPRGFAIKFYTEEGNYDLVGSSTPVFF
                     IRDAAKFGDLVHAQKRQPDTGMRSNDMLWDFWTLSPESAHQVTMLMSGRGTPRTLRHM
                     NGYGSHTFSWVNAAGEKFWVKYHFKTAQGIETYSEAEALRMAAEDPDAHRRDLRRAIE
                     AGEGPEWRLQMQVMPFEDAAGYRFNPFDITKVWPHGDYPPIEVGRLVLDRNPEHYVDE
                     VERSGFSPANLVPGIGLSPDEMLMGRIFSYHDTHLNRIGADYEQLPINSARGPAGSSG
                     GATAEAIWSVDPAEIRSYAEARHTDDDDFGQAGTLYRDVMDEAQRAELVENVVRHASA
                     EVSDEVQMRVVDYWTQVDSVLGARVDAGLAATP"
     gene            1766..2779
                     /locus_tag="VFB52_08860"
     CDS             1766..2779
                     /locus_tag="VFB52_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO06484.1"
                     /translation="MNGMDAEQQIAIPTPPEPRVALAELEDGQRVRGVYAVRERELRR
                     KRNGEPWLRLSVGDASGAAEAVSWEGAEELYAICEKGCAVFVTGTFENSERWGAKIKL
                     IELRAAEPHEYESADLAPASEVSAEVLEAGLRELLATIEDTHLRELLDYFFGELSPSW
                     HRFREAPAAKVYHQAYQHGLLEHSLSVAQAVNAASGFFPGIDREVAVAGALLHDVGKL
                     VAYNDDPLAIDLTDAGRLEGEIPLGYYAVRRQIEEIDGFDPGLARCLLHIILSHHGSL
                     EHGSPVVPATREAVLVHMIDNLGGRLGSFDRIERQLPAGESWSGFDRALSGSAYFGNR
                     EAA"
     gene            complement(2776..4260)
                     /locus_tag="VFB52_08865"
     CDS             complement(2776..4260)
                     /locus_tag="VFB52_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZO06485.1"
                     /translation="MEPEEELPKALPQPLAAALVFVAAGAVLVLEILAVRLLAPYVGL
                     TLETTTSIIGAALAGIAMGAALGGYLADRTDTRLLVVLLLIGGGALAMLTLPIVRLLG
                     DALQGEGDAAALFITAVALVPAAAVLSAVSPAVAHLQLHDLRVSGTVVGRLSAWATAG
                     ALIGTFGTGFVLVPLIPVSTAILLTGIVVALFGLGLAAWGGILPGRAQLGAVVGLAAV
                     AAISLVPDSPCDTESDYHCVALEKVANETSGYELLLDNVHNSYVDLEDPTRLGYSYTR
                     WIAESIRGSFPGREPLDAVFVGGGGFTLPRWLLATRPGSTAHVLEVDGRVVEFVEDKL
                     GLETSEDLQVSVGDARVTALNLPDGSADLVVGDAFSGYTVPWHLTTVEWLDEVQRVLR
                     PNGIYTLNLIDEQPMAFVEAELATLLDRFRFVAMIAVLDELGELAGGNVVVLASDRPI
                     ALPVHKGPPEAAFLRRPQIEEVAANAPVLRDDHAPVDQLLTVTY"
     gene            complement(4319..4672)
                     /locus_tag="VFB52_08870"
     CDS             complement(4319..4672)
                     /locus_tag="VFB52_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06486.1"
                     /translation="MDFLRNLFGNLTSGIIRLLVAVGIIAAVGYFLVRPALDTTNKAI
                     DSYNKSFRESFGSNGVDFNDIGKSIDDVNRQVQREIRKSLKAAKQEGNPKKLVRCIQH
                     AKGDVQKIQKCTVKF"
     gene            4703..>4908
                     /locus_tag="VFB52_08875"
     CDS             4703..>4908
                     /locus_tag="VFB52_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06487.1"
                     /translation="MAKDTEKLIRQLSLISFLMAQGRPVSALEIKREVEGYSDMNDDA
                     FARRFYADRAELESLGIQLGVEKPG"
BASE COUNT          806 a         1708 c         1680 g          714 t
ORIGIN      
        1 gagcagctcg tgacggtcga cgttgccgct gcgcaggtcg acgaccggct gggcggcgag
       61 cagcagccgc tcctcggaga gcgccgcacg gatccgcttg cgccagctct gggactcggc
      121 ttcgctgcgc gggcgcgcct cgccgggagg agaggcgaag aggcggctca ggtggcgccc
      181 aagcacctcc tcgcgtttga agccgaaaac ctgaacggcg gactcgttcc actcgatcac
      241 gcgaccctga tcgtcggtga cgatcagggc atcgaccgcg attgcgagcg gttcgtccat
      301 gtccaagcct cggtttatac gggagttttc ttgaggactt agccctttcc gtccacgtcg
      361 aataccacga cggttcctca aagcgccagg cgtggagccg ctcagaggcc accacgccgc
      421 gcgccgaact ccgcgaagca gtaaaaaagg atgtgtgacc gacgagcaga ccgtgcagaa
      481 gctgcagagc ttcagccgcg accagggcag cgagcgggtc gtccatgcca agggcagcgc
      541 cgcccacggc ttcttcgaag cgaccgagga cgtctcccag ttcaccaagg cgggcctttt
      601 ccgcaagggc gcgcgcacgc cgctcttcct gcgcttctcg accgaagccg gtgagcacgg
      661 ctccgccgac accgcccgcg acccgcgcgg cttcgcgatc aagttctaca ccgaggaagg
      721 caactacgac ctggtcggca gcagcacccc ggtgttcttc atccgcgacg ccgccaagtt
      781 cggcgacctc gtgcacgcgc agaagcgcca gccggacacg ggcatgcgct ccaacgacat
      841 gctctgggac ttctggacgc tctcgccgga gagcgcccac caggtgacga tgctgatgtc
      901 cggccgcggc acgccgcgca ccctgcgcca catgaacggc tacggcagcc acaccttctc
      961 ctgggtcaac gcggccggcg agaagttctg ggtcaagtac cacttcaaga ccgcacaggg
     1021 gatcgagacc tacagcgaag ccgaggccct gcgaatggcg gccgaggacc ccgacgccca
     1081 ccgccgcgac ctgcgccgcg cgatcgaggc gggcgaaggc cccgagtggc gcctgcagat
     1141 gcaggtgatg ccgttcgagg acgccgccgg ctaccgcttc aacccgttcg acatcaccaa
     1201 ggtctggccg cacggggact acccgccgat cgaggtcggc cgcctcgtcc tcgaccgcaa
     1261 ccccgagcac tacgtcgacg aggtcgagcg ctccggcttc tcgcccgcga acctggtgcc
     1321 ggggatcggg ctcagcccgg acgagatgct gatgggccgg atcttcagct accacgacac
     1381 ccacctgaac cggatcggag ccgactacga gcagctgccg atcaactcgg cgcgcggccc
     1441 ggccggctcc tccggcggag cgaccgccga ggcgatctgg tcggtcgacc cggccgagat
     1501 ccgcagctac gccgaggccc gccacaccga tgacgacgac ttcggccagg ccggcaccct
     1561 ctaccgcgac gtgatggacg aggcccagcg cgccgagctc gtcgagaacg tcgtccgcca
     1621 cgccagcgcc gaagtcagcg acgaggtgca gatgcgggtg gtcgactact ggacccaggt
     1681 cgactccgtg ctcggcgccc gcgtcgacgc gggtctcgcg gcgacccctt agcgctgtcc
     1741 gcagccgtct ctaatttgag acgggatgaa cgggatggac gccgagcagc agatcgcgat
     1801 cccgacgccg ccggaaccgc gggtggcgct ggccgagctg gaggatgggc agcgggtgcg
     1861 gggcgtctac gcggtgcgcg agcgcgagct gcgccgcaag cgcaacggcg agccctggct
     1921 gcggctcagc gtcggcgacg ccagcggcgc ggccgaggcg gtctcctggg agggcgccga
     1981 ggagctctac gcaatctgcg agaagggctg cgcggtcttc gtcaccggca cgttcgagaa
     2041 cagcgaacgc tggggcgcga agatcaagct gatcgagctg cgcgcggccg agccccacga
     2101 gtacgagtcg gccgacctgg cgcccgcctc cgaagtctcc gccgaggtgc tcgaggcggg
     2161 cctgcgcgag ctgctggcga cgatcgagga cacccacctg cgcgaactgc tcgactactt
     2221 cttcggcgag ctctcgccga gctggcaccg cttccgcgag gcgccggcgg cgaaggtcta
     2281 ccaccaggcc taccagcacg gcctgctcga gcactcgctc tcggtcgcgc aggcggtcaa
     2341 cgccgcctcc ggcttcttcc ccgggatcga ccgcgaagtc gcggtcgccg gcgcgctcct
     2401 gcacgacgtc ggcaagctcg tcgcctacaa cgacgacccg ctggcgatcg acctcaccga
     2461 cgccggccgg ctcgaaggcg agatcccgct cggctactac gccgtgcgcc ggcagatcga
     2521 ggagatcgac ggcttcgacc ccggcctcgc ccgctgcctt ctccacatca tcctcagcca
     2581 ccacggctcg ctcgaacatg gctctccggt cgtccccgcc acccgcgagg ccgtcctcgt
     2641 ccacatgatc gacaacctcg gcggccgcct cggcagcttc gaccggatcg agcgccagct
     2701 ccccgccggc gagtcctggt cgggcttcga ccgcgcgctc tccggctccg cctacttcgg
     2761 caaccgcgaa gcggcctagt acgtaaccgt cagcagctgg tcgaccggag cgtggtcgtc
     2821 gcgcaggacc ggcgcgttgg cggcgacttc ttcgatctgc gggcggcgca ggaaggcggc
     2881 ttccgggggt cctttgtgga ccgggagagc gatcgggcgg tcggaggcga ggacgacgac
     2941 gttgccgccg gccagttcgc cgagttcgtc caggaccgcg atcatcgcga cgaagcgaaa
     3001 gcggtcgagc agggtcgcca gctcggcctc gacgaacgcc atcggctgtt cgtcgatcag
     3061 gttgagcgtg tagatcccgt tggggcggag cacccgctgg acttcgtcga gccactcgac
     3121 cgtggtcagg tgccagggaa ccgtgtagcc gctgaaggcg tcgccgacga ccaggtcggc
     3181 gcttccgtcg ggcaggttga gcgcggtcac ccgcgcgtcg ccgacgctca cctgcagatc
     3241 ctccgaggtc tccagcccga gcttgtcttc gacgaactcg accacgcggc cgtcgacctc
     3301 gagcacgtgc gcggtcgagc ccgggcgggt ggcgagcagc cagcgaggca gcgtgaagcc
     3361 gccgccgccg acgaagaccg cgtcgagcgg ctcacggccg gggaagctgc cgcggatcga
     3421 ctcggcgatc cagcgcgtgt agctgtagcc gagccgggtc gggtcttcga ggtcgacgta
     3481 ggagttgtgg acgttgtcga gcagcagctc gtagccgctg gtctcgtttg cgaccttttc
     3541 gagcgcgacg cagtggtagt cgctctcggt gtcgcagggc gagtcgggga cgagcgagat
     3601 cgccgcgacc gcggccagcc cgacgaccgc gccgagctgc gccctgccgg gcaggatccc
     3661 gccccaggcg gcgagcccga ggccgaagag ggcgacgacg atcccggtca gcaggatcgc
     3721 ggtgctgacc gggatcaacg ggaccagcac gaagccggtg ccgaaggttc cgatcagcgc
     3781 gcccgccgtc gcccaggcag agagccgccc gaccacggtg ccgctgaccc gcaggtcgtg
     3841 cagctgcagg tgggcgaccg cgggtgagac cgcgctcagc accgccgcgg ccgggaccag
     3901 ggcgaccgcg gtgatgaaca gggcggcggc gtcaccctcg ccttgcagcg cgtcgccgag
     3961 caggcgcacg atcggcagcg tcagcatcgc cagggcgccg ccgccgatca gcagcagcac
     4021 gaccagcagg cgggtgtcgg tgcggtcggc gaggtagccg ccgagcgctg cgcccatcgc
     4081 gatcccggcc agggccgcgc cgatgatcga ggtggtcgtc tccagggtga ggccgacgta
     4141 aggcgccagc agccgcaccg cgaggatctc caggaccagg accgcgccgg cggcgacgaa
     4201 gaccagcgcc gcggcaagcg gctgcggcaa cgccttcggc agctcctcct caggctccat
     4261 cggcgctgac aatacctcct acgtacctaa ttggtacgaa attccttcgc ggtgcgcctc
     4321 agaatttgac cgtgcacttc tggattttct gcacgtcgcc tttggcgtgc tggatgcagc
     4381 gcaccagttt cttcgggttg ccctcctgct tggccgcttt cagcgatttg cggatctcgc
     4441 gctggacctg gcggttgacg tcgtcgatcg atttaccgat gtcgttgaag tcgacgccgt
     4501 tgctgccgaa cgattcgcgg aagctcttgt tgtagctgtc gatcgccttg ttggtggtgt
     4561 cgagagcggg ccggacgagg aaatagccga ccgcggcgat gatcccgacc gccaccaata
     4621 gccggatgat gccggaagta aggttcccga agaggttgcg caggaagtcc atgccgtgcg
     4681 ccgcagacta tcctgcgcag cagtggcgaa ggacaccgag aagctgatcc gccagctctc
     4741 tctgatctcg ttcctgatgg cccagggccg cccggtctcg gccctggaga tcaagcgcga
     4801 ggtcgagggc tacagcgaca tgaacgacga cgcgttcgcg cgtcgttttt acgctgaccg
     4861 cgcggagctg gagagcctcg ggattcagct cggggtcgag aagcccgg
//
LOCUS       DASRIG010000326         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1977246, whole genome shotgun sequence.
ACCESSION   DASRIG010000326 DASRIG010000000
VERSION     DASRIG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977246"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFB52_08880"
     CDS             <1..387
                     /locus_tag="VFB52_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06488.1"
                     /translation="ILMLVAGLGMIVGGILAPVDAARPGLIGGGLGVGLAGLLLAYLD
                     APSRREKPASGMAPAKARILDGAVLPGSVAGYQMVELTLEVRPKNGIPFQATRKFSAG
                     RLGRIEAGREIDVVYDLTNPDRVELP"
     gene            complement(392..1438)
                     /locus_tag="VFB52_08885"
     CDS             complement(392..1438)
                     /locus_tag="VFB52_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06489.1"
                     /translation="MRTLKRRPTILTLSFLAALLLVPVSANAATNFGSRLNHDPANSG
                     ECEPLGTCTLVSFIHPSDPEGDPYAGGAPVDGVITKFRIRAYVLNEPTQVTFRLADIN
                     RPDPNDESTALATAAGTGPTVTLQVNEDLIEAPIQEFPGRLPVKKGQHLAIDGGKYLL
                     ATINDSGQEFSYVFSPPLVDGSGARGSLESTGELLVAATIEPDADGDGFGDETQDACP
                     TQGTTQGACDLTKPAVSGLKVKDGKVAYKLSEAATVTLKLEKKAPGRKVGKKCVKPTA
                     KNKAKKACNRWRPVGKAFGGTGKAGANSVTLPKGKNLAPGTYKLTLTAVDAAGNKTVQ
                     ATKFTVAPKLKKGK"
     gene            complement(1467..>2387)
                     /locus_tag="VFB52_08890"
     CDS             complement(1467..>2387)
                     /locus_tag="VFB52_08890"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZO06490.1"
                     /translation="ASSSSGNINCVWSPDGTEIIYTLGVFGEGDLARRDLSGTLLSTP
                     SSWGVAEHFDGNADWATNFSPSCEAKGAAIPVNGFTSVQLGCTDPDFGFGIAPPGPAP
                     VGDSSLEIVKPPANGVLGGINDDGQVIYTPNKDFKGTDTFTYTGDDQVSQAPPATVTI
                     QVGQTATSDKSPPKITGLKLSAKRWRLGGKLASISKDPVGTTISFRLNEAARATLGFQ
                     RGKPGRQVGKKCVKQTAANAGRKACTRYVGAGNLGFQAKAAVNKVKFQGRVSASKTLA
                     PGTYRVVVSARDAAGNKSSKTGPTFTIVAN"
BASE COUNT          286 a          786 c          842 g          473 t
ORIGIN      
        1 attctgatgc tggtcgccgg gctggggatg atcgtcggcg ggatcctcgc cccggtcgac
       61 gccgcgcgcc ccggcctgat cggcggtgga cttggcgtcg gcctcgcggg actcctcctc
      121 gcctacctcg acgcgccctc acgccgtgag aagccggcct ccgggatggc gcccgcgaag
      181 gcccgcatcc tcgacggcgc cgtcctgccc ggctcggtcg ccggctacca gatggtcgag
      241 ctgaccctcg aagtccgccc caagaacggc atccccttcc aggccacccg caagttctcc
      301 gccggccgcc tcggccggat cgaggccggc cgcgagatcg acgtcgtcta cgacctcacc
      361 aaccccgacc gggtcgaact gccctagcgg gctatttccc ttttttcagt ttcggcgcga
      421 ccgtgaactt ggtcgcctgg accgttttgt tgccggccgc gtcgaccgcg gtcagggtca
      481 gcttgtaggt gccgggggcg aggtttttgc ccttcggcag ggtgaccgag ttggcgccgg
      541 ctttaccggt gccgccgaag gctttgccga ccgggcgcca acggttgcag gcttttttgg
      601 ctttgttttt cgccgtcggc ttgacgcact ttttgccgac cttgcgaccc ggggctttct
      661 tctcgagctt cagggtgacc gtcgccgcct cgctcaattt gtaggcgacc ttgccgtctt
      721 tgactttcag tccgctgacg gcgggtttgg tcaggtcgca ggcaccctgc gtggttccct
      781 gggtcgggca ggcgtcctgg gtctcgtcgc cgaagccgtc gccgtcggcg tcgggctcga
      841 tcgtcgccgc gaccagcagc tcgccggtcg attcgagtga gccgcgggcg ccgctgccgt
      901 cgaccagcgg cggcgagaac acgtagctga actcctggcc gctgtcgttg atcgtcgcca
      961 gcaggtattt gccgccgtcg atcgccaggt gctgtccttt tttgaccggc aggcggccgg
     1021 ggaactcctg gatcggcgct tcgatcaggt cttcgttcac ctgcagggtc acggtcggcc
     1081 cggtgccggc cgcggtcgcc agtgcggtcg actcgtcgtt cgggtcgggg cggttgatgt
     1141 ccgcgagccg gaaggtcacc tgggtcggtt cgttgagcac gtaggcgcgg atgcggaatt
     1201 tggtgatcac gccgtcgacc ggggcgccgc ccgcgtaggg gtcgccttcc gggtcgctcg
     1261 ggtggatgaa ggagaccagc gtgcaggttc cgagcggctc gcactcgccg gagttggccg
     1321 ggtcgtggtt gaggcggcta ccgaagttgg tcgccgcatt ggcgctgacc gggaccagca
     1381 gcagcgccgc gaggaagctc agggtgagaa tggtcgggcg gcgcttcagg gttcgcatcg
     1441 gtgttccttt cgggttctgc tgctgctcag ttggcgacga tcgtgaacgt cgggccggtc
     1501 ttcgaggact tgttgccggc cgcgtcgcga gcggagacga cgacccggta ggtgccgggc
     1561 gcgagcgtct tgctggcgct gacccggccc tggaacttga ccttgttgac ggcggccttc
     1621 gcctggaagc caaggttgcc ggcgccgacg tagcgggtgc acgctttgcg ccccgcgttg
     1681 gctgccgtct gcttcacgca cttcttgccg acctggcgcc cgggcttgcc gcgctggaag
     1741 ccgagcgtcg cgcgcgccgc ctcgttcagc cggaaggaga tcgtcgtccc caccgggtct
     1801 ttggagatgc tcgccagctt gccgcccagg cgccagcgct tcgccgagag cttcagcccg
     1861 gtgatcttcg gtggcgactt gtcgctcgtc gccgtctggc cgacctggat cgtcaccgtc
     1921 gccggcggcg cctgcgagac ctggtcgtcg ccggtgtagg tgaaggtgtc ggtccctttg
     1981 aagtctttgt tcggggtgta gatcacctgg ccgtcgtcgt tgatcccacc gagtacgccg
     2041 ttcgccggcg gtttgacgat ttccagcgag ctgtcgccga ccggcgccgg ccccggtggg
     2101 gcgatcccga agccgaagtc ggggtcggtg cagccgagct gcacgctcgt gaacccgttg
     2161 accggaatcg ccgcgccctt cgcttcgcag ctgggagaaa agttggtcgc ccagtcggcg
     2221 ttgccgtcga agtgttcggc gacgccccag ctcgacgggg tcgagagcag cgtcccgctc
     2281 aggtcccggc gggcgaggtc gccttcgccg aagacgccga gcgtgtagat gatctcggtc
     2341 ccgtccggcg accagacgca gttgatgttg cccgacgaag aggaggc
//
LOCUS       DASRIG010000327         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2234966, whole genome shotgun sequence.
ACCESSION   DASRIG010000327 DASRIG010000000
VERSION     DASRIG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234966"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFB52_08895"
     CDS             complement(<1..706)
                     /locus_tag="VFB52_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO06491.1"
                     /translation="MKRAACAALAVLLAALIAGCGGGSKNTDVATVAAAEVDEPATGT
                     LRVFAYEDSVTPEMIAPFEAQNPDLNVETATFDSNSEAAAKLGGGFKADVVEVCLDEA
                     EPLLKRELLRPLDTAAIPEWDKIAFTESEGVRQGGNPIMVPLSAGPEGVIVNTDEVPA
                     AQIDSYADLYDPAYAGRTALEGDYALPPIAITALSMGIEDPMNMDAAQLEEVGDYMDE
                     HRDQFRALWGSDSDLVN"
     gene            complement(703..1800)
                     /locus_tag="VFB52_08900"
     CDS             complement(703..1800)
                     /locus_tag="VFB52_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06492.1"
                     /translation="MIELRALRKDFGQATAVNSIDLEIGAGEFFSLLGPSGCGKTTTL
                     RMIAGFEEPSSGQILLAGEDVTWTSPRKRKVNMVFQDYALFPHMTVRENVAFGLKIKK
                     VGAAEAKQKIAAMLAAVRLEGYEERLPAQLSGGQRQRVALARALVNEPEALLLDEPLG
                     ALDLKLRKEMQLELKAIQGRTGTTFVYVTHDQEEALTMSDRIAVMNGGNVEQVADPRS
                     LYEQPRTTFVAGFIGTSNLLEIAVDDRAGGIASANLGEGMRLTCPDRGGAGQVTVTVR
                     PEMIKLVEEGAAPDGSLVRGKVLENVYLGSMTQLIVEIQTGERLIVHQLNDESGREPI
                     APGAPVLLAWQTEHSFVIEGETAAPAETVEA"
     gene            complement(1810..3204)
                     /locus_tag="VFB52_08905"
     CDS             complement(1810..3204)
                     /locus_tag="VFB52_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZO06493.1"
                     /translation="MNPATRENQGRVRNAEQLCSHGITGLRSAALEIAAAGLDACDPY
                     EATVAAVAPSADGIMVGGIEHKLVPGAKLIVLGAGKATLPIAVALEDTLGDRLDGGVI
                     IVRRGEEAGLQRLEVMAADHPLPTADSEAAARRLLDFADQLGPDDLVLACFTGGSSAL
                     ASLGPVGVPLEHKRELHERLIGSGMPIGQVNAVRKHVSEVKGGRLAARIAPARIVNLT
                     VSDVAGDHLDVITDLTVPDTSTTEDAIAILQRHGLWDEIPGTVVQHLSGAGERSPHSP
                     ELNTSRIWTELLVTGEIACAAMVEAAVGCGLRPVILTTTMEGEAREMGRFVADLCRSS
                     AARGYPFPPGTVLVGCGGENVVRLAPGTGFGGGGPGQEAAVAAALELRDAPVAALFMD
                     TDGSDGGTDAAGAIVDGLTAARSAELSLDLVSALHAHGVRPPLVDLDDLIVTGPTGTN
                     VNDLFVAVIGEPED"
     gene            complement(3201..>3494)
                     /locus_tag="VFB52_08910"
     CDS             complement(3201..>3494)
                     /locus_tag="VFB52_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZO06494.1"
                     /translation="WPDQRFHRAIIEATESARLDRAYADLQSEIVLCLVQLRPAYDQP
                     AEVAAEHEELLAPIIAGDAAKAEALMRAHLEEATENLVRLHGAKADASEDGQA"
BASE COUNT          444 a         1216 c         1285 g          549 t
ORIGIN      
        1 ggttgaccag gtcggagtcc gagccccaga gcgcccggaa ctggtcgcgg tgctcgtcca
       61 tgtaatcgcc gacttcttcg agctgggcgg cgtccatgtt catcgggtcc tcgatcccca
      121 tcgagagcgc cgtgatcgcg atcggcggca gcgcgtagtc gccctccagc gccgtgcgcc
      181 cggcgtaggc ggggtcgtag aggtcggcgt aggagtcgat ctgggcggcc gggacctcgt
      241 cggtgttgac gatcacgccc tcgggcccgg cggagagcgg caccatgatc gggttgccgc
      301 cctggcgcac gccctccgat tcggtgaagg cgatcttgtc ccattccggg atcgcggcgg
      361 tgtcgagcgg gcgcagcagc tcgcgtttga gcagcggctc ggcctcgtcg aggcagacct
      421 cgaccacgtc ggccttgaag ccgccgccga gcttcgccgc ggcctccgag ttcgagtcga
      481 aggtcgcggt ttcgacgttc aggtccgggt tctgggcttc gaacggcgcg atcatctccg
      541 gcgtcaccga gtcctcgtag gcgaacaccc ggagcgtgcc cgtggccggc tcgtcgactt
      601 cggccgcggc gaccgtcgcc acgtcggtgt tcttgctgcc cccgccacag ccggcgatca
      661 gcgccgcgag caaaaccgcc agcgctgcgc aggccgcgcg cttcatgctt cgaccgtctc
      721 ggcgggcgcc gcggtctcgc cctcgatcac gaagctgtgc tcggtctgcc aggcgagcag
      781 gaccggagcg ccgggggcga tcggctcgcg gccgctctcg tcgttcagct ggtggacgat
      841 cagccgctcg ccggtctgga tctcgacgat cagctgggtc atcgagccga ggtagacgtt
      901 ctccagcacc ttgcctcgga ccagcgagcc gtcgggcgcg gcgccctcct cgaccagctt
      961 gatcatctcc gggcgcacgg tcacggtcac ctggccggcg ccgccgcggt cggggcaggt
     1021 cagccgcatg ccctcgccga ggttggcgct ggcgatgcca ccggcgcggt cgtcgaccgc
     1081 gatctcgagc aggttcgagg tgccgatgaa gccggcgacg aaggtcgtgc gcggctgctc
     1141 gtagaggctg cgcgggtcgg cgacctgctc gacgttgccg ccgttcatca ccgcgatccg
     1201 gtcgctcatc gtcagcgcct cttcctgatc atgggtgacg tatacgaagg tggtcccggt
     1261 gcggccctgg atcgctttca gttcgagctg catctccttg cgcagcttca ggtcgagcgc
     1321 gcccagcggc tcgtcgagca gcagcgcctc gggctcgttg accagcgccc gggcgagcgc
     1381 gacgcgctgg cgctggccgc cggagagctg cgccggcagc cgctcctcgt agccctcgag
     1441 ccgcaccgcg gcgagcatcg cggcgatctt ctgtttcgcc tccgccgccc cgaccttctt
     1501 gatcttgagg ccgaaggcga cgttctcgcg cacggtcatg tgcgggaaca gggcgtagtc
     1561 ctggaagacc atgttgacct tgcgcttgcg cggcgaggtc caggtcacgt cctcaccggc
     1621 gagcaggatc tggccgctgc tcggctcctc gaagccggcg atcatccgca gcgtcgtcgt
     1681 cttgccgcaa ccgctcggcc cgagcaagga gaagaactcg ccggcgccga tctcgaggtc
     1741 gatcgagttg accgccgtgg cttgaccgaa gtctttcctc agggcgcgga gctcgatcat
     1801 cgccatcggc tagtcctccg gctccccgat gaccgcgacg aagaggtcgt tgacgttggt
     1861 cccggtcggc ccggtgacga tcaggtcgtc gaggtcgacc agcggcgggc gcaccccgtg
     1921 cgcgtgcagg gcggagacga ggtcgaggct gagctcggcg gagcgcgcgg cggtcagccc
     1981 gtcgacgatc gcgccggcgg cgtcggtgcc gccgtcggag ccatcggtgt ccatgaagag
     2041 ggcggcgacc ggcgcgtcgc gcagctccag ggcggctgca accgcggcct cctggccggg
     2101 accgccgccg ccgaagccgg tgccgggggc gagccggacg acgttctccc caccgcagcc
     2161 gaccagcacc gtgccgggcg ggaacggata gccgcgggcg gcgctggagc ggcagaggtc
     2221 ggcgacgaag cggcccatct cgcgcgcctc accctccatc gtcgtggtca ggatcaccgg
     2281 ccgcaggccg cacccgacag cggcctcgac catcgccgcg caggcgatct cgccggtgac
     2341 caggagctcg gtccagatcc ggctggtgtt gagctcgggc gagtgcgggg agcgctcgcc
     2401 ggcgccgctg aggtgctgga cgacggtgcc ggggatctcg tcccagagcc cgtgccgctg
     2461 caggatcgcg atcgcgtcct cggtggtcga ggtgtccggc acggtgaggt cggtgatcac
     2521 gtcgaggtgg tcgccggcga cgtcggagac ggtcaggttg acgatccggg ccggggcgat
     2581 ccgcgcggcc agccggccgc ccttgacttc ggagacgtgc ttgcgaaccg cgttgacctg
     2641 gccgatcggc atgcccgagc caatcaggcg ctcgtgcagc tcgcgcttgt gttcgagcgg
     2701 gacgccgacc gggccgaggc tggcgagcgc cgagctgccg ccggtgaaac aggcgagcac
     2761 gaggtcgtcg gggccgagct ggtcggcgaa gtcgagcagg cggcgggcgg cggcctcgct
     2821 gtcggcggtc ggcagcgggt ggtcggcggc catcacctcg agccgctgga gccccgcctc
     2881 ttcgccgcgg cggacgatga tcacgccgcc gtcgaggcgg tcaccgaggg tgtcttcgag
     2941 cgcgaccgcg atcggcagcg tcgccttgcc ggcgccgagc acgatcagct tcgcgccggg
     3001 cacgagcttg tgctcgatcc caccgaccat gatcccgtcg gcgctcggcg cgaccgcggc
     3061 gaccgtcgcc tcgtacggat cgcaggcgtc gaggccggcg gcggcaatct ccagcgcggc
     3121 gctgcgcagc ccggtgatgc cgtgggagca aagctgctcg gcgttgcgga cccgcccttg
     3181 gttctcgcgg gtcgccgggt tcacgcctgc ccgtcttcgg acgcgtcggc tttggcgccg
     3241 tgcagccgca ccaggttctc ggtcgcctcc tcgaggtggg cgcgcatcag cgcctcggcc
     3301 ttggcggcgt cgccggcgat gatcggagcc agcagctcct cgtgctcggc ggcgacctcg
     3361 gccggctggt cgtaggccgg gcgcagctgc acgaggcaga gcacgatctc cgactgcagg
     3421 tcggcgtagg cgcggtcgag gcgagcgctc tcggtcgcct cgatgatcgc ccggtggaag
     3481 cgctggtcgg gcca
//
LOCUS       DASRIG010000328         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3273833, whole genome shotgun sequence.
ACCESSION   DASRIG010000328 DASRIG010000000
VERSION     DASRIG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273833"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(665..1885)
                     /locus_tag="VFB52_08915"
     CDS             complement(665..1885)
                     /locus_tag="VFB52_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06495.1"
                     /translation="MNLLVIFRVAFRALARNKMRSALTMLGIIIGVAAVIAMVSVGQG
                     AQASIQEQISSMGTNMLFVFPGSMNQGGVRGGTGTTTNTLTVEDIEAIRRECPSVAMA
                     SPIVRAGAQIVFGNQNWNTSVQGVNEEFPQIRKWAVQSGEFFTEADIRTAARVAVLGQ
                     TVVDNLFPGADPLGQMVRVRELPFRVIGVMAKRGQGARGEDQDDMIFAPYTAVQKKLL
                     SITFVHFANVSAISPNATYAAEQQITDLLRQRHKLGPNQESDFSVRNQTDWAESAAAT
                     NQIMTLLLGSIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARSTAVRTQFLIE
                     SIVLSLLGGLFGILLGVSLSLLIPQMLGWPTLVSVTAILGSVFFSAAVGIFFGYYPAR
                     KAAGLDPIDALRYE"
     gene            complement(2035..>2362)
                     /locus_tag="VFB52_08920"
     CDS             complement(2035..>2362)
                     /locus_tag="VFB52_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06496.1"
                     /translation="SGGQQQRVAIARALVNRPALILADEPTGNLDSRTSVEVMEIFQR
                     LNRDQGITLVLVTHEPDIAQYSKRVIVFKDGKINRDYPVADSRNAAEEIKLLPETIDD
                     DDDDDE"
BASE COUNT          427 a          664 c          839 g          432 t
ORIGIN      
        1 cctctggggt tgaatttctc gttggggcct tgcaaggccg ccttgcccgg ggcgatgtta
       61 gtggagagcg tggtgagcgc gaaggccacc gcgaaaatcg cgacaatcat cggtttcaag
      121 gctttcattc tgtctccttc aacggactga tgaacagtct caggggagtg gtccgggaga
      181 ccacgtaatt gagccgttgc tcacggagcg aaatcgccgt gcgaaagggc acgcggccgg
      241 gctgatcgtt cgattcgtcc cctaattctc aagcgacgtc ccgcgggaaa cacaaaaact
      301 cctccctgcc cccggccatg aggctctggg gggcgtgatc tcttgcctga agggcaaggc
      361 tcgaccgggc cgtcagcgat gacggcttgc tatctggtcg gcgcgaatag agagatagct
      421 ttactgcgga ggcaacgtca gtgtgcggtg gcgaagctca gctcacgaca aatcgcggct
      481 gaatgctggc cgtacgagtt cgattcggaa cgcggggcgc gtcacgagaa cgggtaaaga
      541 actccggttg tcagtgatgc gaggaagtcg tcggtggcaa cgcgggcgtg aaggtactat
      601 cggcggcgcg gcttgtcaat ccatttaata ttagtcgcgc tactcgcagt caagacgagt
      661 aacgctactc atagcggagc gcgtcgatcg ggtcgaggcc ggcggccttg cgcgccgggt
      721 agtagccgaa gaagatgccg acggcggcgg agaagaagac cgagccgagg atcgcggtca
      781 ccgagacgag cgtcggccac ccgagcatct gcgggatgag gagcgagagc gagacgccga
      841 ggaggatgcc gaagaggccg ccgaggagac tgaggacgat cgactcgatg aggaactggg
      901 tgcggacggc cgtcgagcgc gcgccgatcg ccatgcggat gccgatctcg cgcgtccgct
      961 cggtgaccga gacgagcatg atattcatga tgccgatccc gccaacgagc agcgagacgc
     1021 tggcgatgct cccgaggagg agcgtcatga tctggttggt ggcggcggcc gactcggccc
     1081 agtcggtctg gttgcggacc gagaaatcgc tctcctggtt cggcccgagc ttgtggcgct
     1141 ggcggagcag gtcggtgatc tgctgctcgg cggcgtaggt cgcgttgggg gagatggccg
     1201 agacgttggc gaagtggacg aaggtgatcg agaggagctt cttctggacg gcggtgtagg
     1261 gggcgaagat catgtcgtcc tgatcttcgc cgcgggcgcc ctggccgcgc ttggccatga
     1321 caccgatgac gcggaagggc aattcgcgga cgcggaccat ctggccgagc gggtcggcgc
     1381 cggggaagag gttatcgacc acggtctggc cgaggacggc gacgcgggcg gcggtgcgga
     1441 tgtcggcctc ggtgaagaac tcgccgctct ggacggccca cttgcggatc tgggggaact
     1501 cctcgttgac gccctggacc gaggtgttcc aattctggtt gccgaagacg atctgcgcgc
     1561 cggcgcggac gatggggctg gccatcgcga cgctcgggca ctcgcggcgg atcgcctcga
     1621 tgtcttcgac cgtgagcgtg ttggtcgtcg tcccggtgcc gccacgcacc ccgccctggt
     1681 tcatcgagcc ggggaagacg aagagcatgt tggtgcccat gctcgagatc tgctcctgga
     1741 tcgaggcctg cgccccctgg ccgacgctga ccatggcgat gaccgcggcg acgccgatga
     1801 tgatcccgag catggtcagc gccgagcgca tcttgttacg cgccagcgcc cggaaggcga
     1861 ctcgaaaaat gactagcaag ttcatcgtcc gctgactcca ccttcaccca tcattctaaa
     1921 acctgcgtct gataacgtaa gtgggtttga tattgtgtca ccaaaaggga gacagggtca
     1981 aggccctcga ccgagcctcg ccgggcgatc tggcgatctg tttcgagttg gtgatcactc
     2041 gtcgtcgtca tcgtcgtcgt caatcgtctc gggaaggagt ttgatctctt cggcggcgtt
     2101 gcgcgaatcg gcgacggggt agtcgcggtt gatcttgccg tccttgaaga cgatgacgcg
     2161 cttggaatac tgagcaatgt cgggctcgtg ggtgacgagc acgagcgtga tgccttggtc
     2221 acggttgagg cgctggaaaa tctccatgac ctcgaccgag gtgcgcgagt cgaggttgcc
     2281 cgtcggctcg tcggccagga tcagcgccgg gcggttgacc agggcgcggg cgatggcgac
     2341 gcgctgctgc tgcccgcccg ag
//
LOCUS       DASRIG010000329         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3273899, whole genome shotgun sequence.
ACCESSION   DASRIG010000329 DASRIG010000000
VERSION     DASRIG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273899"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2000)
                     /locus_tag="VFB52_08925"
     CDS             complement(<1..>2000)
                     /locus_tag="VFB52_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZO06497.1"
                     /translation="SGGMRCGVTKDSYSTVRALTFVLPSGTLIDTGAPRAAEQFATTE
                     PELVAGLEAIRDEIRADAELSERIRRKFAIKNTTGYRLCAFLDADEPLEIFRRLLVGS
                     EGTLAFLAEATFETVPLPTVTTTAWIHFPSIDAAIAPVNDLVNSGATAVELMVAPALI
                     TAAWNMVGAPQEWKELPPESAVLLVEFGGAEEADLAEQVAGAEAILAGHETIRPIGFT
                     RELEEIELAWRVREGLHGLIGKLRLPGTSLIVEDVCVPPERIAEGARDLQALLGEHGF
                     LPGVAGHASAGNLHFMLTPDFAKQEDLDRYEAFMGKLVELIVGKYDGSLKAEHGTGVN
                     MAPYVEREWGEKATALMWRVKELADPDGVLSPGVVLNRDPGAHLRNLKTTPAIEESAT
                     SCVECGFCEPVCPSRDLTTTPRQRIVLRREMARQPVGSPVWQALEKEYEYEALETCAA
                     DGSCQIACPVGIDTGALVKELRREGHPPGAERLEMAKARRWNLVEGASRLGLRVGGPL
                     ARATKRGAALPKAGTAKLPATIHTGAAAVYVPSCTNRIFAGSPAAALVEVSSRAGLPV
                     WIPPDVEGSCCGLPWSSKGFGEAHRYKANEMTAKLWEWSGEGALPIVIDAASCTNATL
                     EPGEGVLSEENAERLGQLEILDSVAWAHDRLLPWLEVGEKVG"
BASE COUNT          265 a          729 c          711 g          295 t
ORIGIN      
        1 agccgacctt ctccccgacc tccagccagg gcagcaggcg gtcgtgggcc caggcgaccg
       61 agtcgaggat ctcgagctgg ccgagccgct cggcgttctc ctcgctgagc acaccctcgc
      121 ccggttcgag cgtcgcgttg gtgcaggagg cggcgtcgat cacgatcggc agcgcgccct
      181 cgccgctcca ctcccagagc tttgcggtca tctcgttggc cttgtagcgg tgcgcctcgc
      241 cgaacccctt cgagctccag ggcagcccgc agcagctgcc ctcgacgtcg ggcgggatcc
      301 agaccggcag gccagcgcga ctggagacct cgaccagggc tgcggccggg gagccggcaa
      361 aaatccggtt cgtgcaggag gggacgtaga ccgcggcggc gccggtatgg atcgtcgccg
      421 gcagcttcgc cgtgcccgcc ttcggcagcg ccgcgccgcg cttggtcgcc cgcgccagcg
      481 ggccgccgac ccgcaggccg agccgcgagg cgccttcgac caggttccag cgccgcgctt
      541 tcgccatctc cagccgttcg gcccctggcg ggtggccctc gcggcgcagc tccttgacca
      601 gcgccccggt gtcgatcccg accgggcagg cgatctggca ggagccgtcg gcggcgcagg
      661 tctccagcgc ctcgtactcg tattccttct ccagcgcctg ccagaccggc gagccgaccg
      721 gctggcgcgc catctcgcgg cgcagcacga tccgctggcg cggcgtcgtg gtcaggtcgc
      781 ggctcgggca aaccggctcg cagaagccgc actcgacaca ggaggtcgcc gactcctcga
      841 tcgccggagt cgtcttcagg ttgcgcaggt gggcgccggg gtcgcggttg aggacgacgc
      901 ccggggagag cacgccgtcg gggtcggcca gctccttcac ccgccacatc agcgcggtcg
      961 ccttctcccc ccactcgcgc tctacataag gggccatgtt gacgccggtc ccgtgctcgg
     1021 ccttcagcga gccgtcgtac ttgccgacga tcagctcgac cagcttgccc atgaacgcct
     1081 cgtagcggtc gaggtcctcc tgcttggcga agtccggggt cagcatgaag tgcaggttgc
     1141 cggcggaggc gtggccggcg acgccgggca ggaagccgtg ttcgccgagc agcgcctgca
     1201 ggtcgcgggc gccctcggcg atccgctccg gcggcacgca gacgtcctcg acgatcagcg
     1261 aggtgcccgg cagccgcagc ttgccgatca gcccgtgcag gccctcgcgc acgcgccagg
     1321 cgagctcgat ctcctccagc tcacgggtga acccgatcgg ccggatcgtc tcgtggccgg
     1381 cgaggatcgc ttcggcgccg gcgacctgct cggccaggtc ggcctcctcg gcgccgccga
     1441 actcgaccag cagcacggcc gactccggcg gcagctcttt ccactcctgc ggcgccccga
     1501 ccatgttcca ggcggcggtg atcagcgccg gcgcgaccat cagctcgacc gcggtcgcgc
     1561 ctgagttgac caggtcgttg accggggcga tcgccgcgtc gatgctcggg aagtggatcc
     1621 aggcggtggt cgtcaccgtt gggagcggca cggtctcgaa ggtcgcctcg gcgaggaagg
     1681 cgagcgtgcc ctccgagccg accagcaggc gccggaagat ctccagcggc tcgtcagcgt
     1741 cgaggaaggc gcagagccgg tacccggtcg tgttcttgat cgcgaacttg cggcggatcc
     1801 gctcgctcag ctcggcgtcg gcgcggatct cgtcgcggat cgcttccagg ccggcgacca
     1861 gctccggctc ggtggtcgcg aactgctcgg cggcgcgcgg ggcgccggtg tcgatcagcg
     1921 tgcccgaggg aaggacgaag gtgagcgcgc gcacggtcga gtaggagtcc ttggtgacgc
     1981 cgcagcgcat cccgcccgag
//
LOCUS       DASRIG010000330         5099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2235490, whole genome shotgun sequence.
ACCESSION   DASRIG010000330 DASRIG010000000
VERSION     DASRIG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235490"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFB52_08930"
     CDS             <1..194
                     /locus_tag="VFB52_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06498.1"
                     /translation="KKDKARVARLIELIPGLRAVNAGPLEMARIVEQLTPMLISVNSR
                     YKVHAGIRLTGLPDTDHWA"
     gene            197..1120
                     /gene="cofD"
                     /locus_tag="VFB52_08935"
     CDS             197..1120
                     /gene="cofD"
                     /locus_tag="VFB52_08935"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZO06499.1"
                     /translation="MRVALLAGGTGGAKLGAGMQEEVGEELAVIANTGDDVETLGVHV
                     SPDPDLVTYWLSGEINEEQGWGIRDDSFTVFERLKQLGAPSWFGLSDRDLAACLYRRQ
                     FIEEGGTPTHAQAQIARGLGVKAEVLPMCDTPVRTKVVTEAGRRDLQEYLILDHGDPA
                     VLGVELEGITEAEPTREVLDALRKADAIVIGPSNPVISIGPILAVPGLREAIAASPAP
                     VVAVSPYVAGQVVKGPTDRFMEAIGRPTTAAGVASLYAGLIDGIVVDEGDPDPPPTDV
                     PHLATATLMEGSAGRARLARIVLDYAEGLAG"
     gene            1146..1784
                     /gene="cofC"
                     /locus_tag="VFB52_08940"
     CDS             1146..1784
                     /gene="cofC"
                     /locus_tag="VFB52_08940"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZO06500.1"
                     /translation="MATAVLPVKRFDEAKQRLTAGYEPERRRALVGAMVGDVLEAIES
                     ARTVERTIVVTGDPIAQELASEFDAEVVPDPGDLGHVQAVLAGIARAEVEGAESVVLL
                     PGDCPLLDPQELDRLLTGVPERYVTIVPDRHGTGTNALVLSPPSAIVPAFGEGSCARH
                     VEAARDAGIPYGVEELASLGLDLDTPADVVALTTQLMSRPGKARRTAKALGI"
     gene            1787..2533
                     /gene="cofE"
                     /locus_tag="VFB52_08945"
     CDS             1787..2533
                     /gene="cofE"
                     /locus_tag="VFB52_08945"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZO06501.1"
                     /translation="MGELHVRSVDGLPEFSEGMAVGTEIAARTELADGDLVVVSQKVV
                     SKAEGRLRRLSSVLPGSEARKLAMVLGKEPALVQLVLDESVEVIRAERTVLIVETVHG
                     LVCANAGIDSSNLPDSDTVCLLPEDPDASARRIRAELSAASGATLGVVIADSFGRAWR
                     LGQAEVAIGCAGLAPLDDWRGRHDSSGRELEATLIAIADEAAGAADLVRDKASGTPAA
                     VVRGLGQYIGAEDGPGAAALRRPREEDLFR"
     gene            complement(2588..2767)
                     /locus_tag="VFB52_08950"
     CDS             complement(2588..2767)
                     /locus_tag="VFB52_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZO06502.1"
                     /translation="MSDEIRNKDDAKGRVKEAAGNLTGDKDLQREGKVDQGAGKAKDG
                     IEKLTDKVKDVLRRD"
     gene            complement(2823..5084)
                     /gene="cofH"
                     /locus_tag="VFB52_08955"
     CDS             complement(2823..5084)
                     /gene="cofH"
                     /locus_tag="VFB52_08955"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZO06503.1"
                     /translation="MRRVTFSRNFTLSLSRTCQCYCKYCAFATHQAHVHAPADVERIL
                     DEALKRNTKELLVLTGEKPEVNPVVAERLAAWGHEDFTSYVAWACERALERGMLPHTN
                     IGVCNHEELAKLREVTASQGLMLESVDERLMETVHAGSPTKHPQARLATIEAAGQLKI
                     PFTTGILVGIGESEESRVESLEALAELHDRYGHIQEVILQNFVPHPRYYGAEVADIAD
                     EASKARWRGEGDDPSVTNLEVPPGSSPSPHEPPSIPGWANPVGIEEIKRLIAETKRLM
                     PDVGVQVPPNLSDFWPELVEAGATDLGGLSANGDHIIPEEPFPSPHQVRKELAPKGYA
                     LTERLCVYPQYMDHEWMEQGVLDVIKLKYWSFIPRKGSGRRSEEVIAPDLAPKAIAKG
                     REGLELSEDELTALFAETRPEVIEEMRVAADELRAELAGDTVTFVVNRNINFTNVCIV
                     GCAFCGFGQGKRSPDAYEVSEPEFAARIEEAVAFGATELCMQGGIHPELTLEEYGRWL
                     ALAKQTAPQLHLHAYSPMEINHACEVSGKGPDEVFEYLLAHGLGSTPGTAAEVLDDGV
                     RQRISPNKLPVARWVEIIEASHRAGLRSTSTVMFGHIEEPRELARHMRVIRGVQERTG
                     GITEFVPLSFIPFNTLLGRTHGIEEISMQENLKHTAAFRLALGRTITNLQASWVKMGL
                     EGATEALRWGVNDLGGTLMEENISRMAGSQHGVRLEPEQLIEAARAAGREPAQRSTLY
                     EVLERYPAPAVAA"
BASE COUNT          726 a         1856 c         1744 g          773 t
ORIGIN      
        1 agaagaagga caaggcgcgg gtcgcgcgcc tgatcgagct gatcccgggc ctgcgcgcgg
       61 tcaacgccgg cccgctggag atggcgcgga tcgtcgagca gctgaccccg atgctgatct
      121 cggtcaacag ccgctacaag gtccacgccg ggatccggct caccgggctc ccggacaccg
      181 accactgggc ctaggcgtgc gcgtcgcgct ccttgccggc ggcaccggcg gcgccaagct
      241 cggcgccggg atgcaggagg aggtcggcga ggaactggcg gtgatcgcca acaccggcga
      301 cgacgtcgag acgctcggcg tccacgtctc ccccgacccc gacctggtca cctactggct
      361 ctccggcgaa atcaacgagg agcagggctg gggcatccgc gacgacagct tcaccgtctt
      421 cgagcggctc aagcagctcg gtgccccgag ctggttcggg ctctccgacc gcgacctcgc
      481 cgcctgcctc taccgccgcc agttcatcga ggagggcggc acgccgaccc acgcccaggc
      541 gcagatcgcg cgcgggctcg gggtcaaggc ggaagtgctg ccgatgtgcg acacgccggt
      601 gcggaccaag gtcgtcaccg aggccgggcg acgcgacctc caggagtacc tgatcctcga
      661 ccacggcgat ccggccgtgc tcggggtcga gctcgagggc atcaccgagg cggagccgac
      721 ccgcgaggtg ctcgacgcgc tgcgcaaggc cgacgcgatc gtgatcggcc cctccaaccc
      781 ggtgatcagc atcggcccga ttctcgccgt gcccgggctg cgcgaggcga tcgccgcctc
      841 ccccgccccg gtcgtcgcgg tcagccccta cgtcgccggg caggtcgtca agggcccgac
      901 cgaccgcttc atggaagcga tcggccgccc gaccacggcc gcgggggtcg cctccctcta
      961 cgccgggctg atcgacggga tcgtggtcga cgagggcgac cccgacccgc cccccaccga
     1021 cgtcccccac ctggcgacgg cgacgctgat ggagggctcc gccggccgcg cccggctcgc
     1081 ccggatcgtg ctcgactacg ccgagggcct cgccggctga gcgggctggc tacgctcggc
     1141 agctgatggc gaccgccgtc ctccccgtca agcgcttcga cgaagccaag cagcgcctca
     1201 ccgccggcta cgagcccgag cggcggcgcg ccctggtcgg cgcgatggtc ggcgacgtgc
     1261 tggaagcgat cgagagcgcc cgcacggtcg agcgcacgat cgtcgtcacc ggcgacccga
     1321 tcgcccagga actggcctcc gagttcgacg ccgaggtcgt ccccgacccc ggcgacctcg
     1381 gccacgtgca ggcggtgctg gcggggatcg cgcgggccga agtcgagggc gccgagagcg
     1441 tcgtgctgct gcccggcgac tgcccgctgc tcgacccgca ggagctcgac cgcctgctga
     1501 ccggcgtgcc cgagcgctac gtcacgatcg tccccgaccg ccacgggacc ggcaccaacg
     1561 cgctggtcct gagcccgccg agcgcgatcg tgcccgcctt cggcgagggc agctgcgccc
     1621 gtcacgtcga ggccgcccgc gacgccggca tcccctacgg ggtcgaggag ctggcctcgc
     1681 tcgggctcga cctcgacacc ccggccgacg tcgtcgccct gaccacgcag ctgatgtccc
     1741 gcccgggcaa ggcccgccgc accgccaagg cgctggggat ctagacgtgg gcgagctgca
     1801 cgtccgcagc gtcgacggcc tgcccgagtt cagcgagggc atggcggtcg ggaccgagat
     1861 cgcggcgcgc accgagctcg ccgacggcga cctcgtcgtc gtctcgcaga aggtcgtctc
     1921 caaggccgag ggccgcctgc gccggctctc ctcggtcctg cccggcagcg aagcgcgcaa
     1981 gctggcgatg gtgctcggca aggagccggc gctggtccag ctggtgctcg acgagagcgt
     2041 cgaggtgatc cgggccgagc gcaccgtgct gatcgtcgaa acggtgcacg gcctggtctg
     2101 cgccaacgcc ggcatcgact cctccaacct gcccgacagc gacaccgtct gcctgctgcc
     2161 cgaggacccc gacgcctccg cccgccgcat ccgcgccgag ctgtccgcgg ccagcggtgc
     2221 cacgctcggg gttgtgatcg ccgacagctt cggccgcgcc tggcggctcg ggcaggccga
     2281 ggtggcgatc ggctgcgccg gcctcgcccc gctcgacgac tggcgcggac gccacgactc
     2341 cagcggccgc gagctcgagg cgaccctgat cgcgatcgcc gacgaggccg caggagcggc
     2401 cgacctggtc cgcgacaagg cgagtggaac gccggcggcg gtcgtgcgcg gcctcggcca
     2461 gtacatcggt gccgaggacg gacccggggc cgccgccctg cgccgtccgc gcgaagagga
     2521 tctcttccgc taagccagcg ccggcgtact gacgtctgtc gccctctggg ctacaaacgt
     2581 cgtgggatca gtcccgtctg aggacgtcct tgaccttgtc ggtcagcttc tcgatcccgt
     2641 ccttggcctt gccggcaccc tggtcgacct tgccctcgcg ctggaggtcc ttgtcgccgg
     2701 tgaggttgcc tgcggcctcc ttgacgcgcc ccttggcgtc gtccttgttg cgtatctcgt
     2761 cgctcacgcg actccttcca gttgttttct gtcaggagac ggctacccgg atggcgccag
     2821 agtcaagcgg cgacggcggg cgccggatac cgctcgagca cctcatagag cgtcgagcgc
     2881 tgggccggtt cgcggccggc ggcacgggcc gcctcgatca gctgctcggg ctccagccgc
     2941 accccgtgtt gggagccggc catccggctg atgttctcct ccatcagcgt gccgccgagg
     3001 tcgttgacgc cccagcgcag cgcctcggtc gccccctcga ggcccatctt cacccagctc
     3061 gcctgcaggt tggtgatcgt cctgcccagc gccaggcgga aggcggcggt gtgcttgagg
     3121 ttctcctgca tcgagatctc ctcgatcccg tgcgtgcggc cgagcagcgt gttgaagggg
     3181 atgaagctga gcgggacgaa ctcggtgatc cccccggtcc gctcctgcac cccgcggatc
     3241 acccgcatgt gacgggccag ctcgcgcggc tcctcgatgt ggccgaacat cacggtcgag
     3301 gtcgagcgca gcccggcccg gtgggaggct tcgatgatct cgacccagcg cgcgaccggc
     3361 agcttgttcg gcgagatccg ctggcgcacg ccgtcgtcga gcacctcggc cgcggtcccc
     3421 ggggtcgagc cgagcccgtg cgcgagcagg tactcgaaga cctcgtcggg gcccttgccg
     3481 gagacctcgc aggcgtggtt gatctccatc ggcgagtagg cgtgcaggtg cagctgcggc
     3541 gccgtctgct tggccagcgc cagccagcgc ccgtactcct ccagggtcag ctccgggtgg
     3601 atacccccct gcatgcatag ctcggtggcc ccgaaggcga ccgcctcctc gatccgcgcg
     3661 gcgaactcgg gctcgctgac ttcgtaggcg tcaggcgagc gcttgccctg gccgaagccg
     3721 cagaaggcgc agccgacgat gcagacgttg gtgaagttga tgttgcggtt gacgacgaag
     3781 gtgaccgtgt cccccgccag ctccgccctc aactcatcag cagcaacccg catctcctcg
     3841 atcacctcag gccgcgtctc cgcgaacagg gcggtcagct cgtcctcaga gagctccaat
     3901 ccctcgcgtc ccttcgcgat cgccttcgga gccaggtcgg gcgcgatgac ctcttcgctg
     3961 cgccgccccg accccttgcg cggaatgaag ctccagtact tcagcttgat cacgtccagg
     4021 accccctgct ccatccactc gtgatccatg tactgcgggt agacgcaaag ccgctccgtc
     4081 agcgcgtagc ccttgggggc cagctccttg cgcacctggt gcggcgacgg gaacggctcc
     4141 tcggggatga tgtgatcgcc attggctgaa agcccgccga ggtccgtggc gccagcctca
     4201 accagctcgg gccagaagtc agagagattc ggcggcacct gcaccccgac gtccggcatc
     4261 agccgtttcg tctccgcaat cagccgcttg atctcctcaa tgcccacggg gttcgcccag
     4321 ccgggaatcg agggcggctc gtggggcgag ggggacgacc ccggaggaac ctccagattt
     4381 gtgacggagg ggtcgtcccc ctcgcccctc cagcgcgctt tcgaagcctc atccgcgatg
     4441 tccgcgacct cggcgccgta gtagcggggg tgcggaacga agttctggag gatcacctct
     4501 tggatgtggc cgtagcggtc gtgcagctcg gcgagcgctt cgagcgattc gacccgcgac
     4561 tcctcggact cgccaatccc gaccaggatc ccggtcgtga aggggatctt cagctggccg
     4621 gcggcctcga tggtggccag gcgcgcttgc ggatgcttgg tgggagagcc ggcgtgaacc
     4681 gtctccatca ggcgctcgtc gaccgactcg agcatcagcc cctgtgacgc cgtcacttca
     4741 cgcagcttgg ccagttcctc gtggttgcag acgccgatgt tggtgtgggg caacatgccg
     4801 cgctccagcg cccgctcgca ggcccaggcg acatagctgg tgaagtcctc gtggccccag
     4861 gcggcgaggc gctcggcgac gaccggattg acctcgggct tctcgccggt caggaccagc
     4921 agctctttcg tgttccgctt cagcgcctcg tcgaggatcc gctcgacgtc tgcaggcgcg
     4981 tggacgtgcg cctggtgggt ggcgaaggcg cagtacttgc agtagcactg acaggtacgc
     5041 gagagcgaga gggtgaagtt tcgggagaaa gtaacccggc gcaccctggc gaggctatc
//
LOCUS       DASRIG010000331         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3830207, whole genome shotgun sequence.
ACCESSION   DASRIG010000331 DASRIG010000000
VERSION     DASRIG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3830207"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFB52_08960"
     CDS             <1..285
                     /locus_tag="VFB52_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZO06504.1"
                     /translation="SAVDLVFTTPEPLDVDQINAALKAAAEGPMKGILAYTEDPIVST
                     DIVGDPHSSIVDAGSTMVIGDGRMAKVIAWYDNEWGYSNRCVELAGKVLA"
     gene            305..1507
                     /locus_tag="VFB52_08965"
     CDS             305..1507
                     /locus_tag="VFB52_08965"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZO06505.1"
                     /translation="MAFAKASVRDAEVEGRRVLVRVDFNVPLADGPDGSRVVADDMRI
                     RAALPTIELLRERGAALVLCSHLGRPKGKVDPALSLKPVGEKLGELLGVEVKQAPEVV
                     GADAETMAAGLGPGEVLLLENTRFEPGETENDTKLAEGLAGLGDLFVNDAFGAAHRAH
                     ASTEGVAQFLPGYAGLLLEREVTELSAVVEAPKRPLVVVLGGAKVSDKVAVIDRFLDV
                     ADRILIGGAMAFSFFRAEGVPTGDSLVEEEGVELAAKAVEKARDSECELVLPVDLVLG
                     REFSAETERRESHGIEVPDGWMGLDVGPRTGKLYGDAVMEAGTVLWNGPMGAFELEPF
                     ATGTKTVAEAVAAAPGTTVIGGGDSVAAIRKFGLDDEVDWLSTGGGASLELLEGKKLP
                     GVEALRDA"
     gene            1500..2249
                     /gene="tpiA"
                     /locus_tag="VFB52_08970"
     CDS             1500..2249
                     /gene="tpiA"
                     /locus_tag="VFB52_08970"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZO06506.1"
                     /translation="MPRTPYIAANWKMNKTVAEAAEFVDALLPRIAATQHDVVICPPF
                     TALSEVVQRRYGTAVKVAAQNMHEEDSGAFTGEVSAPMLVELDVEAVILGHSERRQFF
                     GETDEALARKVPAALAHDLEPILCVGESEAARDGGETEEVLERQLQADLAHVEGPDLA
                     KVVIAYEPIWAIGTGKVATPEQAQEACAYIRDVVSMRGGVADDLRVLYGGSVKPGNAA
                     ELLALPDVDGALVGGAALDAEDFAAIVEAAG"
     gene            2251..>2781
                     /locus_tag="VFB52_08975"
     CDS             2251..>2781
                     /locus_tag="VFB52_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZO06507.1"
                     /translation="MTPLVPSLALVILDGWGLAEPGPGNAISLAQTPVFDRLWGTYPH
                     TQLSAQGPDVGLPVGQMGNSEVGHLNLGAGAIVKQDLARIDDAIADGSFFDNPALLAA
                     CERARESPRGRLHLLGLVSDGGVHSGWEHLEACVELASQEGVPDVVFHAFTDGRDTLP
                     HGGKAYLEELELWLRAA"
BASE COUNT          406 a          976 c         1013 g          386 t
ORIGIN      
        1 tcggccgtcg acctcgtctt caccacgccc gagccgctcg atgtcgacca gatcaacgcc
       61 gctctgaagg ccgccgcgga gggcccgatg aagggcatcc tcgcctacac cgaggacccg
      121 atcgtctcca ccgacatcgt cggcgacccg cactcctcga tcgtcgacgc gggctcgacg
      181 atggtgatcg gcgacggccg catggccaag gtgatcgcct ggtacgacaa cgagtggggc
      241 tactcgaacc gctgcgtgga gctggccggc aaggtcctcg cctagccgga gcggggacgg
      301 cgtcgtggct ttcgcgaagg caagcgtccg cgacgccgag gtcgagggcc ggcgcgtcct
      361 cgtccgcgtt gacttcaacg tgccgctggc cgacggtccc gacgggagtc gggtcgtcgc
      421 cgacgacatg cggatccggg cggcgctgcc gacgatcgaa ctgctgcgcg aacgcggcgc
      481 cgctctcgtc ctctgctccc acctggggcg gccgaagggc aaggtcgacc cggcgctctc
      541 gctgaagccg gtcggcgaga agctcggcga gctgctcggg gtcgaggtca agcaggcgcc
      601 cgaggtggtt ggcgccgacg ccgagacgat ggctgccggg ctcggccccg gcgaggtgct
      661 gctgctcgag aacacccgct tcgagccggg cgagaccgaa aacgacacga agctggccga
      721 gggccttgcc gggcttggcg acctcttcgt caacgacgcc ttcggcgccg cccaccgcgc
      781 tcacgccagc accgagggcg tcgcccagtt cctgccgggc tacgccgggc tgctgctcga
      841 gcgcgaggtg accgagctga gcgccgtggt cgaggcgccc aagcggccgc tggtcgtcgt
      901 gctcggcggc gccaaggtct ccgacaaggt cgcggtgatc gaccgcttcc tcgacgtcgc
      961 cgaccgcatc ctgatcggcg gcgcgatggc cttcagcttc ttccgcgccg agggcgtacc
     1021 gaccggcgac tcgctggtcg aggaggaggg cgtcgagctg gccgcgaagg cggtcgagaa
     1081 ggcgcgcgac tccgagtgcg agctggtcct gccggtcgac ctcgtgctcg ggcgcgagtt
     1141 cagcgccgag accgagcgtc gcgagagcca cgggatcgaa gtcccggacg gctggatggg
     1201 gctcgacgtc ggcccccgca ccggcaagct ctacggcgac gcggtgatgg aggcgggcac
     1261 ggtgctctgg aacggcccga tgggcgcctt cgagctggaa ccctttgcaa ccggtacgaa
     1321 gaccgtcgcc gaggcggtcg cggcggcgcc gggcacgacc gtgatcgggg gcggcgactc
     1381 ggttgccgcg atccgcaagt tcggcctcga cgacgaggtc gattggcttt cgaccggagg
     1441 aggagcatcc ttggagctgc tggagggaaa gaagctgccc ggagtggagg ccttgcgcga
     1501 tgcctaggac cccatacata gccgccaact ggaagatgaa caagaccgtc gccgaggcgg
     1561 ccgagttcgt cgacgcgctg ctgccgcgga tcgccgcgac ccagcacgac gtcgtcatct
     1621 gcccgccatt cacggcgctc tccgaggtcg tgcagcgccg ctatggcacc gcggtcaagg
     1681 ttgctgccca gaacatgcac gaggaggact cgggtgcgtt caccggcgag gtctcagcgc
     1741 cgatgctggt cgagctcgac gtcgaggcgg tgatcctcgg gcactccgag cgccgtcagt
     1801 tcttcggcga gaccgacgag gcgctggccc gcaaggtgcc ggccgcgctc gcccacgacc
     1861 tcgagccgat cctctgcgtc ggcgagagcg aggctgcgcg cgacggcggc gagaccgagg
     1921 aagtgctgga gcgccagctg caggccgacc tcgcccacgt cgagggcccg gacctggcca
     1981 aggtcgtgat cgcctacgag ccgatctggg cgatcggcac cggcaaggtc gcgacccccg
     2041 agcaggccca ggaggcgtgc gcctacatcc gcgacgtggt cagcatgcgc ggcggagtcg
     2101 ccgacgacct gcgggtgctc tacggcggct cggtcaagcc cggcaacgcc gccgagctgc
     2161 tcgcgctgcc agacgtcgat ggtgccctgg tcggtggcgc ggcgctcgac gccgaggatt
     2221 ttgcggcgat cgtggaggcg gcgggctgag gtgacgcccc tggtcccgag cctggcgctg
     2281 gtcatcctcg acggctgggg cctcgccgag ccgggccccg gcaacgcgat ctcgctcgcc
     2341 cagacgccgg tcttcgaccg cctctggggg acctacccgc acacccagct ctctgcccag
     2401 ggccccgacg tcgggctccc ggtcggccag atgggcaact ccgaggtcgg ccacctcaac
     2461 ctcggcgccg gcgcgatcgt caaacaggac ctcgcccgga tcgacgacgc gatcgccgac
     2521 ggcagcttct tcgacaaccc ggcgctgctc gccgcctgcg agcgggcgcg ggaaagcccg
     2581 cgcgggcgcc tgcacctgct cgggctggtc tccgacggcg gcgtgcactc cggctgggag
     2641 cacctcgagg cgtgcgtcga gctggcctcg caggagggcg tgcccgacgt cgtcttccac
     2701 gccttcaccg acggccgcga cacgctcccg cacgggggca aggcctacct cgaggagctg
     2761 gagctgtggc tgcgtgcagc g
//
LOCUS       DASRIG010000332         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3274216, whole genome shotgun sequence.
ACCESSION   DASRIG010000332 DASRIG010000000
VERSION     DASRIG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274216"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFB52_08980"
     CDS             complement(<1..435)
                     /locus_tag="VFB52_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06508.1"
                     /translation="MSDDFRIRPATDADAELLFELILELATYEKLADQVVGDAATLRR
                     SLFEQRAAEALIVERGGEAVGYAIFFTTFSTFETRSGVWLEDVYVRPEHRRGGIGAAV
                     MEHLAELALERGHVRLEWCALTWNEPALRFYDQLGARRLDEWR"
     gene            complement(377..1984)
                     /locus_tag="VFB52_08985"
     CDS             complement(377..1984)
                     /locus_tag="VFB52_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06509.1"
                     /translation="MRQFVEKLQQRPHLVLSACCLALVLVMASVTSVNLALEDIAVDL
                     KASGADLTWIADAYTVALAALVLTFGAFGDDFGRRRALVIGTLVFGVAATLAALTTSV
                     SMLIFWRAVMGVGAAMIMPSTLSTITAVFPADQRPRAVAIWAGFASAGAVIGLLMSGA
                     LLEFFSWESTFFAIAALAAVSLLATLLCVPNTHSTEEVRPDLFGSLLTAVGIGALVYG
                     IIEGADKGWGETLPVVSYVVAVVALTLWAIRDSRIRLPILDPRLFKLRGLSSGTLTLV
                     LLFLGAFGFFYVGLQYVQLVLGFGPLTAALAFLPIAAVVMPLSALTPWLGERLGDKAI
                     MAGGLFLMAVSLLLVTRLDAESGYVSFLIPMLVFAAGLAISSTPSTNVVVASLPATKQ
                     GVASALNDVTRETGAALGIALLGSLFTSGYSDAVAPATTQLPAGAAAAVEDSAAGGLH
                     VAASPKLGEAGAVLEQQVRESFMTGMHDAFLVGAIVLLVGIAYVLLRAPGKGAVPEGE
                     AEDALGLAGERRLPHSPGDGRRRRAAV"
     gene            2044..2622
                     /locus_tag="VFB52_08990"
     CDS             2044..2622
                     /locus_tag="VFB52_08990"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06510.1"
                     /translation="MIDSEAASRRELAAGAALAVLAANGSRGLTHRAVDSEAGLPPGS
                     TSNYFRTRSSLLQAALRRHIELDLPPASLVDQIGDAKLSKKEARDLFAAGLGRVIDPE
                     ARPMIAARYELVLEATRRDELRREYARARERVLGLTELVLRASGCKSPSEHAEQMAIL
                     FDGIALDQLLESERALDSKGVEKALDRWLKSC"
     gene            complement(2623..2946)
                     /locus_tag="VFB52_08995"
     CDS             complement(2623..2946)
                     /locus_tag="VFB52_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZO06511.1"
                     /translation="MSSLALTLGALRGAVVSVLRDPRTRALPFIAFGIVLAGTVFYWI
                     AEDWTLIQALYFSVVTLTTVGFGDLAPTTDLARLVTVFYILIGVGVLLALITAVIQAY
                     VKDQA"
     gene            3013..3513
                     /locus_tag="VFB52_09000"
     CDS             3013..3513
                     /locus_tag="VFB52_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZO06512.1"
                     /translation="METIDRLLSNNESFAAGLPERHLSVEPSLHLAIVTCMDSRLDVF
                     GALGLGEGEAHVLRNAGGVITDDVIRSLALSQRKLGTREVMLIHHTDCGLEKVSDDGF
                     RAELQDATGVAPAFAIESFSDVDADVRQSVLRVRRSAFVPHRDVVRGFVYEVDTHRLR
                     EIEVDD"
BASE COUNT          610 a         1200 c         1276 g          476 t
ORIGIN      
        1 ccgccactca tccagccgcc gggcgccgag ctggtcgtag aagcgcagcg ccggctcgtt
       61 ccaggtcagc gcgcaccatt cgagccgcac gtgcccacgc tccagggcca gctccgccag
      121 gtgctccatc accgcggcgc cgatcccgcc acggcggtgc tccgggcgca cgtagacatc
      181 ctcgagccag accccggagc gggtctcgaa ggtcgagaag gtggtgaaaa agatcgcgta
      241 gccaaccgcc tctcccccgc gctcgacgat cagcgcctcc gctgcccgct gctcgaacaa
      301 cgagcggcgc agcgtcgcgg cgtcgccgac gacctgatcg gcgagtttct cgtaggtggc
      361 gagctcgagg atcagctcaa acagcagctc ggcgtcggcg tccgtcgccg ggcgaatgcg
      421 gaagtcgtcg ctcacccgcc aagcctaggg cgtcctcggc ttcgccctcc ggcacggcgc
      481 ctttgccggg ggcgcgcagc aggacgtaag cgatcccaac caggaggacg atcgctccga
      541 ccaggaaggc gtcgtgcatg ccggtcatga acgattcgcg gacctgctgt tcgagcaccg
      601 cgccggcttc gccgagcttc gggctggcgg cgacgtgcag cccgcccgcg gccgagtcct
      661 cgaccgcggc ggcggctcct gccggaagct gggtggtcgc cggggcgacg gcgtcgctgt
      721 agccgctggt gaagagcgag ccgagcaggg cgatgccaag cgcggcgccg gtctcccggg
      781 tgacgtcgtt gagcgccgag gcgaccccct gcttggtcgc cggcagcgag gcgaccacga
      841 cgttggtcga gggcgtgctg gagatcgcca ggccggcggc gaagaccagc atcgggatca
      901 agaaggagac gtagccggac tcggcgtcga gccgggtcac cagcagcagc gagaccgcca
      961 ttaggaagag gccgccggcc atgatcgcct tgtcgccgag cctctccccc aaccacggcg
     1021 tcagcgccga gagcggcatc actacggcgg cgatcgggag aaaggcgagc gccgcggtca
     1081 gtggcccgaa cccgagcacc agctggacgt actggaggcc gacgtagaag aagccgaagg
     1141 cgccgaggaa gagcaggacc agggtcaggg tgccgctgct gaggccgcgc agcttgaaca
     1201 ggcgcgggtc gaggatcggc aagcggatcc ggctgtcgcg gatcgcccag agggtgagcg
     1261 cgacgacggc gacgacgtag gagaccaccg gcagcgtctc cccccagccc ttgtcggcac
     1321 cctcgatgat cccgtagacg agcgcgccga tcccgaccgc ggtaagcagc gagccgaaca
     1381 aatcgggacg gacctcctcg gtgctgtggg tgttcggcac gcagagcagc gtcgccagca
     1441 gcgagaccgc ggcgagggcg gcgatcgcga agaaggtcga ctcccaggag aagaactcga
     1501 gcagggcgcc ggacatcagc aggccgatca cggcgccggc gctggcgaag ccggcccaga
     1561 tcgcgaccgc gcgcggacgc tgatcggccg ggaagacggc ggtgatcgtc gagagcgtgc
     1621 tcggcatgat catcgccgcg ccgacaccca tcaccgcgcg ccagaagatc agcatgctga
     1681 ccgaggtggt gagcgcagcg agggtcgccg cgactccgaa gacgagggtg ccgatgacca
     1741 gcgcgcggcg gcggccgaag tcgtcgccga aggcgccgaa ggtgagcacc agtgcggcca
     1801 gcgcgaccgt gtaggcgtcg gcgatccagg tgaggtcggc gcctgacgct ttcaggtcga
     1861 cggcgatgtc ctcgagcgcc aggttgacgc tggtcaccga ggccatcacc aggaccaggg
     1921 cgaggcagca ggcggagaga accaggtgcg ggcgctgctg aagcttttct acgaactgcc
     1981 gcatttgcgg ggaatgtaca gcagattcac tacatctgta gtggatacgg tacaaatata
     2041 cgagtgatcg attcagaagc tgccagtcgg cgggagctgg cggcaggtgc ggccttggcc
     2101 gtgctcgccg ccaatggttc gcgcgggctc acccaccgcg cggtcgactc cgaggcgggg
     2161 ctgccgccgg gctcgacctc gaactacttc cgcacccgct cctcactgct gcaggcagcg
     2221 ctgcgccgcc acatcgaact cgacctgcct cctgcctccc tggtcgatca gatcggcgac
     2281 gccaagctgt cgaaaaagga ggcgcgcgac ctcttcgccg ccgggctcgg ccgcgtgatc
     2341 gacccggagg cgcggccgat gatcgccgcc cgctacgagc tggtgctgga ggcgacccgc
     2401 cgcgacgagc tgcgccgcga gtacgcccgc gcccgcgaac gcgtcctcgg cctgaccgaa
     2461 ctggtcctcc gcgccagcgg ctgcaagagc cccagcgagc acgccgagca gatggcgatc
     2521 ctgttcgacg gaatcgccct cgaccagttg ctcgaaagcg agcgggcgct cgattcgaag
     2581 ggcgtcgaaa aggcgctcga ccgctggctg aagagctgct gactaggcct ggtctttgac
     2641 gtaggcctgg ataacggcgg tgatcagggc gaggaggacg ccgactccga tcaggatgta
     2701 gaagacggtg acgaggcggg cgaggtcggt ggtcggggcg aggtctccga agccgacggt
     2761 ggtgagggtg acgacggaga agtagagcgc ctggatcagg gtccagtcct cggcgatcca
     2821 gtagaagacc gtgccggcga ggacgatgcc gaaggcgatg aacgggagcg cccgggtgcg
     2881 cgggtcgcgc agcaccgaga cgaccgcgcc gcgcagggcc ccgagcgtga gtgcgagcga
     2941 gctcacgggc tggagattcg ctcccggagc gggcttccct ccaccgggtg gccgaccatc
     3001 taggctgccc gcgtggagac gatcgaccgc ctgctgtcca acaacgagtc cttcgccgca
     3061 gggctgcccg agcgccacct ctcggttgaa ccgagcctgc acctggcgat cgtcacctgc
     3121 atggactccc gcctcgacgt cttcggcgcg ctcgggctcg gcgagggtga ggcgcacgtg
     3181 ctgcgtaacg ccggcggcgt gatcaccgac gacgtgatcc gttcgctggc gctctcccag
     3241 cgcaagctcg gcacccgcga ggtgatgctg atccaccaca ccgactgcgg cctggaaaag
     3301 gtcagcgacg acggcttccg ggccgagctg caggacgcga ccggggtcgc ccccgccttc
     3361 gcgatcgagt cgttcagcga cgtcgacgcc gacgtgcgcc agtcggtcct gcgcgtgcgg
     3421 cgctcggcct tcgtgcccca ccgcgacgtc gtgcgcggct tcgtctacga agtcgacacc
     3481 caccgcctgc gcgagatcga ggttgacgac tagctctccc tacgggtcgt gggattcgcc
     3541 ggtctcggcc gagtcggtcg cg
//
LOCUS       DASRIG010000333         8789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3831745, whole genome shotgun sequence.
ACCESSION   DASRIG010000333 DASRIG010000000
VERSION     DASRIG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3831745"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFB52_09005"
     CDS             complement(<1..188)
                     /locus_tag="VFB52_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06513.1"
                     /translation="MSTIGIFLVLAGGTAFAAAQLGKNSVGAKQLKKNAVTTAKLKKN
                     AVTQAKIKKGAVTGGKIA"
     gene            complement(303..1346)
                     /locus_tag="VFB52_09010"
     CDS             complement(303..1346)
                     /locus_tag="VFB52_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZO06514.1"
                     /translation="MGDEATGRGDDGIDAPALESWFAENLPDAALPLRFERIAGGHSN
                     LTYRVTDDAGRSWALRRPPLGKRLGSAHDMAREHRVVSALGPTEVPVAPVVGLCEDDA
                     VNGAPFYVMEFVEGPVLRGLAEAEAFPEESDRRAIGERVADTLVAIHEVDPDAVGLGD
                     LGRKQDYVARQLRRWQGQWEKSKTRELPVIDRLHERLAARVPEQGPATLVHGDYRLDN
                     MILTESGEVAAVLDWELCTLGDPLADVGQLVCYWAARGDDPIGLGQPANLAPGFPDRE
                     ELVARYGERSGRDLSDLDFFVALGYWKLAIIIEGVYARYTAGGYGKVDDGVEYFARLT
                     EQLAAEGERVEAR"
     gene            1435..2625
                     /locus_tag="VFB52_09015"
     CDS             1435..2625
                     /locus_tag="VFB52_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO06515.1"
                     /translation="MLTAMAVIGVGRFIQQRQDFEDQIARSYQVEMAARVQIAEGEDS
                     AATRAVVSDQVERREQLQDDISSGTRDTAILVAAGLIAGLTGALLLFSGLIASLRRPL
                     EDLVDASARLAAGDLSTRVEVGGLSETAALGRAFNEMADQLEKDAAEREQLDRLKDEF
                     VLTASHELRSPLTSVQGFAELLMLERERLSPKQVETVEIILDNCRHLGRLLNDLLDLA
                     RSDAGRLALRREPTELAPLIDDVVRTMRAQTDAANQALTEQIEPNLPPVYVEPGRIRQ
                     ILVNLITNAHEYSPERASVQVSARVVGTDVEISVSDDGPGIPEPQLEHIFDRFVRGDA
                     GLTQRVGGTGLGLAISKSLVELHGGTIRAESAVGVGSTFSIRLPVSPSEAAVAPSPAT
                     AEPS"
     gene            2625..2984
                     /locus_tag="VFB52_09020"
     CDS             2625..2984
                     /locus_tag="VFB52_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO06516.1"
                     /translation="MPRVLVADDSETILLLMRTRLELAGYEVETAADGQEVTELVAGT
                     GPPDLLLLDAMMPRKSGIDALRELRAAGVETPALIVSAHQNPDDADAANDVEIDGYVT
                     KPIDFDALLEQVAALTR"
     gene            complement(2997..4499)
                     /locus_tag="VFB52_09025"
     CDS             complement(2997..4499)
                     /locus_tag="VFB52_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZO06517.1"
                     /translation="MTAFKDLGLSPDIQQAIDELGFDEPTPIQEQAIPELLGGHDVIG
                     QAQTGTGKTAAFGLPLLQYLDPDNDEVQAVVLTPTRELCIQVTQALRTFAEHLDIEIV
                     AVFGGAPIRSQQSQLRSGAHVVVATVGRMKDLMSRRSLVLTAARYVVLDEADEMLDLG
                     FIEDVEKILRMCPSGRQTALFSATMPPPIQKLAEGYMYDPVTIKITPKQLTVDKISQA
                     FVEVPAKEKAARLVELLKTEEPEQAIIFTRTKIGASKLEKHLKDKGLDVKALHGDLSQ
                     GVRDGVMISFKDHRVKLLVATDIAARGLDVEHVTHVINYDVPNSSETYVHRIGRTGRV
                     GRTGRAITFVTPDQRDEIGRIERDVKTKIGEWETPEERLEHAPPPRRRERSERRREVE
                     KVEASTPVEATGNGDAPVVENGDAAEEPKRDTVKLFVNRGERSGIEEEDLRWALREGA
                     VLPDEDVHDVRVLHRFSFVEVAPDQAERTVEFLDGTKLKGKEIRLEIAKS"
     gene            complement(4522..5469)
                     /locus_tag="VFB52_09030"
     CDS             complement(4522..5469)
                     /locus_tag="VFB52_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO06518.1"
                     /translation="MQAIRIDEFGGPEVLQCVEVEDPTPGEGEVVIEVARSGVNFADT
                     HASRNDYLAPQELPLIPGAEVSGRTPDGRRVAALVGNGGYAQKVAVREAQLIPIPDQV
                     DDDVAAGALLQGLTAMALVQRCARIEPGETIVVEAAAGGTGSLSVQLAKRAGARVIGL
                     ASSAEKRAAVEALGADATVDSGAAELGAAIRAANDGKRVDAVLHMSGGEAFDQELSVL
                     APLGRMVVFGNASRDQRDVATAALLHGSKAVIGFWLVHLLSRPDLLLPMIGELLGQLA
                     AGDLEIRIGGVYPLSEAARAHEDLISRRSTGKLLLDPSS"
     gene            5500..6420
                     /locus_tag="VFB52_09035"
     CDS             5500..6420
                     /locus_tag="VFB52_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZO06519.1"
                     /translation="MALTVVGSIAFDAVKTPFGERERMLGGAAVHFSLAASFFTEVRP
                     VGPVGDDFGPEQFAVLENRGVLTDDVERVAGGQSFFWRGRYEYDLNVAHTEDTQLGVF
                     GEFEPKLSEAAKCADTLFLANIQPDLQRQVRAQCGDASFSGLDSMNLWIETAKDSLLA
                     AIGEVDCLVVNDAEIRMLTGESNLARAARAVMDLGPRAVVAKQGEYGAALFTADGFFA
                     LPGFPLEDVRDPTGAGDSFAGGFFGYLDGLDGPADEGELRRAMGYGTVLASFNVEEFG
                     TERVARLTREEIDERFEALRRMTQFDALPA"
     gene            6541..7935
                     /locus_tag="VFB52_09040"
     CDS             6541..7935
                     /locus_tag="VFB52_09040"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZO06520.1"
                     /translation="MATEQQIQDANAPGATGAESLTVTDNRTGKTYELPIEDGTIRGM
                     DLRQIKVDEDEFGLMSFDPAFTNTASCRSSVTFIDGDKGILEHRGYSIEQLCEKSTFL
                     EVAYLLIFGELPTGPQLDRWTFDITHHTYVHEDLKEFFKGFRYDAHPMGMLLAGVGAL
                     STFYPDAKQIDDPEERYMSAVRLIAKVPTLAAFSYRHNMGFPYVYPDNELNYAENFLS
                     MMFKKTEQKHIPKKALSRALDVLFILHADHEQNCSTNAVRGVGSSDVDPYSAVAAGVA
                     ALYGPLHGGANEAVLKMLREIGSVDNIPGFLEKVKAREAKLMGFGHRVYKNYDPRARI
                     IQQHMDEVFAATEYNPLVEIARELEKRALDDEYFTSRKLYPNVDFYSGIIYEAMEIPT
                     NMFTVLFSIPRTAGWVAQWMEMHEDPATKIARPRQIYTGARELDYVGIDDRDGPEKIL
                     GPPAKRPKRPRRGA"
     gene            7961..8320
                     /locus_tag="VFB52_09045"
     CDS             7961..8320
                     /locus_tag="VFB52_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06521.1"
                     /translation="MSRPAWKLTIRHGSEVSHVGFDDLGEAVASMRERALAIRAEGPR
                     KTASMIREFKPEDQVHARLELSGRGLLRKPVAGVDVRGDGAFMPYRGGLGREELDPSG
                     HDTPFDLVRETLEAEAR"
     gene            8317..8649
                     /locus_tag="VFB52_09050"
     CDS             8317..8649
                     /locus_tag="VFB52_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06522.1"
                     /translation="MSKTTDIPKGSYAAGERVQIPAGAEAPFTVFINGVEQQPGVDYE
                     LEGNEVVFTRPIIKEQMNTGRWLAMYLGLFGTYRKDEKVDLQFTREGKVELVPDLPLI
                     PYPDAKSK"
BASE COUNT         1376 a         3085 c         2977 g         1351 t
ORIGIN      
        1 tcggcgatct tcccaccggt caccgcgccc ttcttgatct tcgcctgggt cacggcgttc
       61 ttcttcagtt tcgccgtggt caccgcgttc ttcttcagct gcttggcgcc gacgctgttc
      121 ttgccgagct gggcggcggc aaatgcggtg ccgccggcaa ggacgagaaa gatgccgatc
      181 gtcgacatca cgtttgcgta cgtgagccgc gggcgaatct gcttcaagac gagcctccta
      241 gaggtaatcg gacgagtcgg gggcgagcgt agctcccggc tcggaggctg aactcagcgc
      301 ggctagcgcg cctcgacccg ctccccctcc gcggccagct gctcggtcag gcgggcgaag
      361 tactcgaccc cgtcgtcgac cttgccgtag ccgccggcgg tgtagcgggc gtagaccccc
      421 tcgatgatga tcgccagctt ccagtagcca agcgcgacga agaagtcgag gtcggagagg
      481 tcgcggccgg agcgctcgcc gtagcgggcg accagctcct cccggtcggg gaagccgggc
      541 gccaggttgg cgggctggcc gagcccgatc gggtcgtcgc cgcgggctgc ccagtaacag
      601 acgagctggc cgacgtcggc gagcgggtcg ccgagcgtgc agagctccca gtcgagcacc
      661 gccgcgacct cgccgctctc ggtgaggatc atgttgtcga gccggtagtc gccgtggacc
      721 agcgtcgccg ggccctgctc gggcacgcgt gccgcgagcc gttcgtgcag ccggtcgatc
      781 accggcagct cgcgcgtctt cgacttctcc cactgcccct gccagcggcg caactgacgg
      841 gcgacgtagt cctgcttgcg gccgaggtcg ccaagcccga cggcgtccgg gtccacctca
      901 tggatcgcaa cgagagtgtc ggcgacgcgc tcgccgatcg cccgccggtc ggactcctcg
      961 gggaacgcct cggcctcggc gaggccgcgc aggaccgggc cctcgacgaa ctccattaca
     1021 tagaagggcg ccccgttgac ggcgtcgtcc tcgcagagcc cgaccaccgg cgcaaccgga
     1081 acctcggtcg gccccaaggc cgagacgacg cggtgctcgc gggccatgtc gtgggcggag
     1141 ccgagccgct tgccgagcgg cggtcggcgc agcgcccagc tgcggccggc gtcgtcggtg
     1201 acccggtagg tcaggttcga gtggccgccg gcgatccgct cgaagcgcag cggcagagcg
     1261 gcgtccggca ggttctcggc aaaccaagat tcgagcgcgg gcgcatcgat cccgtcgtcc
     1321 ccccggcctg tagcctcgtc ccccatcgcc gctcaagcat attcacgacg ggggcgacgg
     1381 ctgagcccat cgctcgccaa ccggctgcgg atcggctttg cgatcggctt tgccgtgctc
     1441 accgcgatgg cggtgatcgg ggtcggccgc ttcatccagc agcgccagga ttttgaggac
     1501 cagatcgccc gctcctacca ggtggagatg gcggctcggg tgcagatcgc cgaaggcgaa
     1561 gactcggcgg cgacgcgagc ggtggtcagc gaccaggtag agcggcgcga gcagctccag
     1621 gacgacatct ccagcggcac ccgcgacacc gcgatccttg tcgccgccgg gttgatcgcg
     1681 gggctgaccg gggcgctgct cctgttcagc ggtctgatcg cctcgctgcg gcgcccgctc
     1741 gaggacctcg tcgacgcctc cgcccggctc gccgccggcg acctcagcac ccgggtcgag
     1801 gtcggcgggc tctcggagac cgcggcgctc gggcgcgcct tcaacgagat ggccgaccag
     1861 ctggagaaag acgccgccga acgcgaacag ctcgaccgcc tcaaagacga gttcgtgctc
     1921 accgcctcgc acgagctgcg cagcccgctg acctcggtcc agggcttcgc cgagctgctg
     1981 atgctggaaa gagagcgcct cagcccgaag caggtggaga cggtcgagat catcctcgac
     2041 aactgccgcc acctcggccg cctgctcaac gacctgctcg acctcgcccg ctccgatgcc
     2101 ggccgcctcg cgctgcgccg cgagccgacc gagctggcgc cgctgatcga cgacgtcgtg
     2161 cgcacgatgc gggcgcagac cgacgccgcc aaccaggcgc tgaccgagca gatcgagccg
     2221 aacctgcctc cggtctacgt cgagcccggc cggatccgcc aaatattggt gaatctgatt
     2281 accaatgccc atgagtactc gcccgaacgg gcgtctgtgc aggtatcggc gcgcgtagtt
     2341 ggaaccgatg tggagatctc tgtgagcgac gacggacccg gcatccccga accccagctt
     2401 gaacacatct tcgaccgctt cgtgcgcggc gacgccggcc tgacccagcg ggtcggcggc
     2461 accgggctcg gcctggcgat ctcaaaatcg ctggtcgagc tgcacggcgg cacgatccgc
     2521 gccgaatccg ccgtcggcgt cggctccacc ttcagcatcc ggctaccggt ctcccccagc
     2581 gaggcggccg tcgctccgag cccggcgacg gcggagccaa gctgatgccg cgcgtcctgg
     2641 tcgccgacga ctcggagacg atcctgctgc tgatgcggac gcggctggag ctcgccggct
     2701 acgaggtcga gaccgccgcc gacggtcagg aggtgaccga gctggtcgcc ggcacaggcc
     2761 cgccggacct gctgctgctc gacgcgatga tgccgcgcaa gtcggggatc gacgccctgc
     2821 gcgagctgcg cgccgccggg gtcgagaccc cggccctgat cgtcagcgcc caccagaacc
     2881 ccgacgacgc cgacgccgcc aacgatgtcg agatcgacgg ctacgtgacc aagccgatcg
     2941 acttcgacgc gctgctcgaa caggtcgccg cgctgacgcg ctagcccggc ttcgggctag
     3001 ctcttggcga tctcgagccg gatctctttg cccttgagct tggtgccgtc gaggaactcg
     3061 acggtgcgct cggcctgatc cggggcgacc tcgacaaacg agaagcgatg caggacgcgg
     3121 acgtcgtgga cgtcctcgtc gggcaggacg gcgccctcgc gcagcgccca gcgcaggtcc
     3181 tcctcctcga tcccgctgcg ctcgccgcgg ttgacgaaga gcttgaccgt gtcgcgcttg
     3241 ggctcctcgg cggcgtcacc gttctcgacc acgggcgcgt cgccgttccc ggtcgcctcg
     3301 accggagtgc tcgcctcgac cttctcgacc tcgcggcggc gctcggagcg ctcgcgccgg
     3361 cgcggagggg gcgcgtgctc gaggcgctcc tccggggttt cccactcgcc gatcttggtc
     3421 tttacgtcgc gctcgatgcg gccgatctcg tcgcgctggt cgggcgtcac gaaggtgatc
     3481 gcgcggccgg tgcgcccgac ccggccggtg cggccgatcc ggtgcacgta ggtctccgag
     3541 gagttcggca cgtcgtagtt gatcacgtgg gtgacgtgct cgacgtcgag cccgcgggcg
     3601 gcgatgtcag tcgccaccag cagcttcacg cggtggtcct tgaaggagat catcacgccg
     3661 tcgcggacgc cctggctgag gtcgccgtgc agcgccttca cgtcgaggcc tttgtccttg
     3721 aggtgcttct ccagcttcga ggcgccgatt ttggtgcggg tgaagatgat cgcctgctcg
     3781 ggttcttcgg tcttcagcag ctcgaccagg cgcgccgcct tctcctttgc gggaacctcg
     3841 acgaaggcct gggagatctt gtccaccgtc agctgtttcg gcgtgatttt gatcgtcacc
     3901 gggtcgtaca tgtagccctc ggcgagcttc tggatcggcg gcggcatggt cgccgagaag
     3961 agtgcggtct ggcgcccgct cgggcacatc cggaggattt tctcgacgtc ctcgatgaaa
     4021 ccgaggtcga gcatctcgtc ggcctcgtcg aggaccacgt agcgggccgc ggtgaggacc
     4081 agcgagcgcc gcgacatcag gtccttcatg cggccgaccg tggcgacgac gacgtgggcg
     4141 ccggagcgca gctgcgactg ctgcgagcgg atcggggcgc cgccgaagac ggcgacgatc
     4201 tcgatgtcga ggtgctcggc gaaggtgcgc agcgcctgcg tcacctgaat gcagagctcg
     4261 cgggtcgggg tgaggacgac cgcctgcacc tcgtcgttgt cgggatcgag gtactgcagc
     4321 agcggcagcc caaaggcagc ggtcttgccg gtcccggtct gcgcctggcc gatcacgtcg
     4381 tgaccgccga gcagctccgg gatcgcctgc tcctgaattg gagtcggctc gtcgaagccg
     4441 agctcgtcga tcgcttgctg gatgtcgggc gagaggccca ggtctttgaa ggcggtcatc
     4501 aggcttccgt gagggggtgg atcaggacga gggatcgagg aggagcttgc cggtgctgcg
     4561 gcgggaaatc aggtcttcgt gggcccgcgc cgcttcggaa agcgggtaga caccgccgat
     4621 cctaatttcg aggtcaccgg ccgcgagctg gccgagcaac tccccgatca tagggagaag
     4681 caggtccgga cggctgagca ggtgcacgag ccagaacccg atcacggcct tcgagccgtg
     4741 caggagggcc gccgtggcga cgtcgcgctg gtcgcgcgag gcgttgccga agacaaccat
     4801 gcggccgagc ggggcgagca ccgagagctc ctggtcgaag gcctcgccgc cgctcatgtg
     4861 gagcacggcg tcgacccgct tgccgtcgtt ggccgcgcgg atcgccgcgc cgagctccgc
     4921 ggcgccggag tcgacggtgg cgtcggcgcc gagcgcctcg accgcggccc gcttctcggc
     4981 gctggaggca agcccgatca cgcgcgcgcc agcccgcttg gcgagctgga ccgagagcga
     5041 gccggtgccg ccggcggcgg cctcgaccac gatcgtctcg cccggctcga tccgcgcaca
     5101 gcgctggacc agcgccatcg cggtcagacc ctgcaggagg gcccccgcgg cgacgtcgtc
     5161 gtcgacctgg tcggggatcg ggatcagctg cgcctcgcgc accgcgacct tctgcgcgta
     5221 gccgccgttg ccgaccaggg cggcgacccg gcggccgtcg ggggtgcggc cgctgacctc
     5281 ggcgcccgga atcagcggca gctcctgcgg cgccaggtag tcgttgcggc tggcgtgggt
     5341 gtcggcgaag ttgacgccgc tgcgggcgac ctcgatcacg acctcgcctt cgccgggcgt
     5401 cgggtcctct acctcgacgc actgcagtac ctcggggccg ccgaactcgt ctatgcgaat
     5461 tgcctgcatt gggtaccgat cctctagggt gcctgcccga tggcactgac tgtagtcgga
     5521 tcgatcgcct ttgacgcggt aaagactcct ttcggggagc gcgagcgcat gctcggcggc
     5581 gctgccgtgc acttctcgct cgccgccagc ttcttcaccg aggtgcggcc ggtcggtccg
     5641 gtcggcgacg atttcgggcc cgagcagttc gccgtgctcg agaaccgcgg cgtgctcacc
     5701 gacgacgtcg agcgggtcgc gggcggccag tccttcttct ggcgcggccg ctacgagtac
     5761 gacttgaacg tcgcccacac cgaggacacc cagctcgggg tcttcggcga gttcgagccg
     5821 aagctctccg aggcggccaa gtgcgccgac accctcttcc tcgccaacat ccagcccgac
     5881 ctgcagcggc aggtccgcgc ccagtgcggc gacgccagct tcagcggcct cgactcgatg
     5941 aacctctgga tcgaaaccgc caaggactcg ctgctggcgg cgatcggcga ggtcgactgc
     6001 ctggtcgtca acgacgccga gatccgcatg ctgaccggcg agtccaacct cgcccgcgcc
     6061 gcccgcgcgg tgatggatct gggaccccgc gcggtcgtcg ccaagcaggg cgagtacggg
     6121 gcggcgctgt tcaccgccga cggcttcttc gccctgcccg ggttcccgct cgaggacgtg
     6181 cgcgacccga ccggggccgg cgacagcttc gccggcggtt tcttcggcta cctcgacggg
     6241 ctcgacgggc ccgccgacga gggcgagctg cgccgggcga tgggctacgg cactgtgctc
     6301 gcctccttca acgtcgagga gttcggcacc gagcgggtcg cacgactgac ccgcgaggag
     6361 atcgacgagc gcttcgaggc gctgcgccgg atgacgcagt tcgacgcgct gcccgcctga
     6421 acggcccgcg cccagactgt cggaaatgcc tagcgcgata gcggctcggc aacagcccgg
     6481 agctatttag ctgcgatact ggcccctccc ccaagagacc gcgcaccccc gaggagggcg
     6541 atggccaccg agcagcagat ccaggatgcg aacgcccccg gcgcaacggg cgcggagtcg
     6601 ctgaccgtca ccgacaaccg caccggaaag acttacgagc ttccgatcga ggacgggacc
     6661 atccgcggga tggacctccg tcagatcaaa gtcgacgagg acgaattcgg gttgatgtcg
     6721 ttcgacccgg ccttcaccaa cacggcttcc tgccgctcct cggtcacctt catcgacggc
     6781 gacaaaggca tcctcgaaca ccgcggctac tcgatcgagc agctgtgtga gaagtcgacc
     6841 ttcctcgagg tcgcctacct gctgatcttc ggcgagctgc cgaccgggcc gcagctggac
     6901 cgctggacct tcgacatcac ccaccacacc tacgtccacg aggacctcaa ggagttcttc
     6961 aagggcttcc gctacgacgc ccacccgatg ggcatgctgc tcgccggcgt cggcgccctc
     7021 tcgaccttct acccggacgc gaagcagatc gacgatccgg aagagcgcta catgtcggcg
     7081 gtgcggctga tcgccaaggt cccgaccctg gccgccttct cctaccggca caacatgggc
     7141 ttcccgtacg tctacccgga caacgaactg aactacgccg agaacttcct ctcgatgatg
     7201 ttcaagaaaa ccgaacagaa gcacatcccc aagaaggcgc tctcgcgtgc cctcgacgtg
     7261 ctcttcatcc tccacgccga ccacgaacag aactgctcga ccaacgcggt ccgcggggtc
     7321 ggctcctccg acgtcgaccc ctactcggcg gtcgcggcgg gcgtggcggc gctctacggc
     7381 ccgctgcacg gcggcgccaa cgaggcggtg ctgaagatgc tgcgggagat cggctcggtc
     7441 gacaacatcc ccggcttcct cgagaaggtg aaagcgcgcg aagccaaact gatgggcttc
     7501 ggccaccggg tgtacaaaaa ctacgacccg cgggcgcgga tcatccagca gcacatggac
     7561 gaggtgttcg ccgcgaccga gtacaacccg ctggtcgaga tcgcccgcga gctcgagaag
     7621 cgggcgctcg acgacgagta cttcacctcg cgcaagctct acccgaacgt cgacttctac
     7681 tcggggatca tctacgaggc gatggagatc cccaccaaca tgttcacggt cctcttctcg
     7741 atcccccgca ccgccggctg ggtggcgcag tggatggaga tgcacgaaga cccggcgacc
     7801 aaaatcgccc ggccgcgcca gatctacacc ggcgcccgtg agctcgacta cgtcgggatc
     7861 gacgaccgcg acggcccgga gaaaatcctc ggcccgccgg cgaaacgccc gaaacgcccg
     7921 cgccgcgggg cctgacgcgc tgaacgcata ggctgggcgg gtgtcccgtc cggcctggaa
     7981 gttgacgatc cgccacggct ccgaggtctc ccacgtgggg ttcgatgacc tcggcgaggc
     8041 ggtggcgtcg atgcgcgagc gggcgctggc gatccgcgcc gagggcccgc gcaagacggc
     8101 cagcatgatc cgcgaattca aacccgagga tcaggtgcac gcgcgcctgg agctcagcgg
     8161 gcgcggtctg ctgcgcaagc cggtcgccgg cgtcgacgtg cgcggcgacg gggccttcat
     8221 gccctaccgc ggcgggctcg ggcgcgagga gctcgatccg agcggccacg acacgccgtt
     8281 cgacctggtg cgcgagactt tagaagcgga ggcgagatga gcaagacgac cgacatcccc
     8341 aaaggcagct acgcggccgg cgagcgggtg cagatcccgg ccggtgccga agcgccgttc
     8401 acggtcttca tcaacggcgt cgagcagcaa ccaggcgtcg actacgaact cgagggcaac
     8461 gaggtcgtct tcacccggcc gatcatcaag gagcagatga acaccggccg ctggctggcg
     8521 atgtacctgg gcctgttcgg cacttaccgc aaggacgaga aggtcgacct ccagttcacc
     8581 cgcgagggca aggtcgaact cgtccccgac ctgcccctca tcccctaccc ggacgccaag
     8641 tcgaagtgaa cctcggcttc gtcctcctct ccgactacag cgaggccgtc aacggcaagc
     8701 tgtacctgac cggcggcgga tggaacgtcc tccgcctgcc cgagctgccc cacgagtggt
     8761 ccttccacat cggcctcggc atcgacgtc
//
LOCUS       DASRIG010000334         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2780959, whole genome shotgun sequence.
ACCESSION   DASRIG010000334 DASRIG010000000
VERSION     DASRIG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780959"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="VFB52_09055"
     CDS             complement(<1..2014)
                     /locus_tag="VFB52_09055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZO06523.1"
                     /translation="MTSFLTDYGVVVALVCAGAAVAYGLIVTQRLLAKSPGNDRMQEI
                     SGAVQEGAQAYLNRQYTIIAAVAVPLAIVLWLLQDWETALGFVIGGVLSGATGFIGMN
                     VSVRANSRVAEAARGGVPPALDVAFKGGSVTGLLVVGLALLGIAGYFGFLVITDHTDK
                     EAVDALIGLGFGGSLISVFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTSVAVMLLGVLTFPDFAREVAIYPLVIGGVAIIASI
                     IGALAVRTKSDRVEGALYRGTIISGVISIAAFYPITDWMMSDPLQFGFAEAGAAAVSV
                     SDLWLCAVIGIAVTALLFVITEFYTSTRFRPVKTIAAASETGHATNIIQGLAQGFQST
                     AAPALVIALAILGANELAGIYGIGIAVMAQLSLCGLIVALDAFGPITDNAGGIAEMAE
                     LPEDVRNVTDPLDAVGNTTKAVTKGYAIGSAALAALVLFAAFKSELAEEAPANFDLNG
                     LFNLTSPDVLIGLIIGGMMVYLFSALAIEAVGRAGGQVVEQVRKQFKEHPGIMDGTEK
                     PEYGETVSIVTLAAQKEMIAPALIPIVVPVIVGLLSVDALGGLLIGVIVVGLFAAISM
                     TAGGGAWDNAKKLIEDGAYGGKGSEAHAAAVTGDTVGDPYKDTAGPA"
     gene            complement(2121..3287)
                     /locus_tag="VFB52_09060"
     CDS             complement(2121..3287)
                     /locus_tag="VFB52_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZO06524.1"
                     /translation="MSLDLDLARERFESSTDFTIGLEEEFAILDPATLELAHRFEDVY
                     AASQKDEVLAESAAGELIASEVEIRSGRAESFAEAVALQTERRSRLFALVAELGLALG
                     ATGTHPWANYLDQRIIDTPHYNRLREELGWVAQRNNTWSLHLHMGIRGADRAIAVCDW
                     LRELLPLLLAVSANSPFLDRRDSGLHTVRTEIFTRTFPRCGVPSPFVDWASYADFIAT
                     LQRTNTVVESTQLWWSVRPHHSFGTVEVRICDAQTRGDEGFALAGLIAACIAQTALDY
                     DELGYDGRGGPLQDREIEENLWRAIRYGADGKMIDFRKGEEIETRALLDSILAWTAPA
                     RSQLGIDPQLPERNGAQRAREALAGGSSIEEIYREAVAETQRTYAGVGQPARPA"
     gene            complement(3310..3978)
                     /locus_tag="VFB52_09065"
     CDS             complement(3310..3978)
                     /locus_tag="VFB52_09065"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZO06525.1"
                     /translation="MVEVIEEFGATESSPVSGERLSLGVAARLSRYLQVLTQAKKMGR
                     ETISSQELSEYTHINPTQIRRDLSGFGKFGKRGVGYNIESLLGEIRKILQTSGQHNIA
                     LFGAGNLGQAIANSPIFADHGFQVVAVFDTDPEVVGTKVGEIEVRELGALDEVVAEEE
                     IVVGVLAVPAEAAQDVADRLVKAGVKIIFNYSEQLLQVPPEVTVHTSSPAVDLLFALY
                     FYLA"
     gene            complement(3966..4238)
                     /locus_tag="VFB52_09070"
     CDS             complement(3966..4238)
                     /locus_tag="VFB52_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZO06526.1"
                     /translation="MNAADQPTVVVYSRPECHLCAEAIERLIALHGEGYRFSLHEIDI
                     ESEDLLLRRYLERIPVVEIDGMVASELILDETAVRERLDTVGAWSR"
     gene            complement(4235..5281)
                     /gene="tsaD"
                     /locus_tag="VFB52_09075"
     CDS             complement(4235..5281)
                     /gene="tsaD"
                     /locus_tag="VFB52_09075"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZO06527.1"
                     /translation="MLILAIETSCDDTCAAVLDGGHVLANTISSQAAAHADFGGVVPE
                     VASRHHLELTAPVVTAALAEAGTSLDQVEAVAVTAGPGLIGALLVGVSTAKAIAAPRR
                     LPLIPVDHLQGHVAANFLEPEPLEPPFLCLVASGGHTLLAAVRDHQGYELLGQTLDDA
                     AGEAFDKSARLLGLGYPGGPAIQKEAEGGDPEAFEMPVAMSRDPGLDFSFSGLKTALV
                     YLCRDLGPEGVEERRADLAASFQAAVVGQLVAKLRRALKGGEWEAVALGGGVAANGPL
                     RAAVGELCEQHGVRLKLVPIPLCTDNAAMIGSAARYATPISYPDYLGYDAFSTGEGAV
                     SVARAAKAAERPAA"
     gene            complement(5304..>5523)
                     /locus_tag="VFB52_09080"
     CDS             complement(5304..>5523)
                     /locus_tag="VFB52_09080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06528.1"
                     /translation="AVAPERRRAGVARSLIARLLEEGGGKLPFTLEVRVSNRPAITMY
                     ERLGFRSAGVRPRYYQDNGEDALIMWLD"
BASE COUNT          879 a         1872 c         1930 g          842 t
ORIGIN      
        1 tcgccggccc cgcggtgtcc ttgtacggat cgcccacggt gtcgcccgtc accgccgccg
       61 catgcgcctc cgagcccttg ccgccgtagg cgccgtcctc gatcagcttc ttcgcgttgt
      121 cccaagcacc gccgccggcg gtcatcgaga tcgcggcgaa gaggccgacg acgatcacgc
      181 cgatcagcag cccgccgagc gcgtcgacgc tgagcaggcc gacgatcacc gggacgacga
      241 tcgggatcag agccggcgcg atcatctctt tctgcgccgc cagggtgacg atgctgaccg
      301 tttccccgta ctcgggtttt tcggtcccgt ccatgatccc cgggtgctct ttgaactgtt
      361 tgcggacctg ctcgaccacc tggccgccgg cacgcccgac cgcctcgatc gcgagcgccg
      421 agaacaggta gaccatcatg ccgccgatga tcagcccgat cagcacgtca gggctggtca
      481 ggttgaacag cccgttgagg tcgaagttgg ccggagcctc ctccgcgagc tcgctcttga
      541 acgccgcgaa cagcaccagc gccgctagcg ccgcggagcc gatcgcgtaa cccttggtca
      601 ccgccttggt cgtgttgccg accgcgtcga gcgggtcggt gacgttgcgg acgtcctccg
      661 gcagctccgc catctcggcg atgccaccgg cgttgtcggt gatcggcccg aaggcgtcga
      721 gggcgacgat cagaccgcag agcgagagct gtgccatcac ggcgatgccg atgccgtaga
      781 tgccggcgag ctcgttggca ccgaggatcg cgagcgcgat caccagcgcc ggagcggcgg
      841 tcgactggaa gccctgagcc aggccctgga tgatgttcgt cgcgtgtccg gtctcggacg
      901 cggcggcgat cgtcttcacc gggcggaagc gggtcgacgt ataaaattcg gtaatcacga
      961 acagcagcgc ggtgaccgcg atgccgatca cggcacagag ccagaggtcg ctgacgctga
     1021 ccgcggcggc accggcttca gcgaagccga actgcagcgg gtcgctcatc atccagtccg
     1081 tgatcgggta gaaggccgcg atcgagatca ccccggagat gatcgtgccg cggtagaggg
     1141 caccctcgac gcggtccgat ttggtccgca ccgcgagcgc cccgatgatc gaggcgatga
     1201 tcgcgacgcc gccgatcacc agcgggtaga tcgcgacctc gcgagcgaag tccgggaagg
     1261 tcagaacgcc gagcagcatc acggcgaccg aggtgaccgc gtaggtctcg aacaggtcgg
     1321 ccgccatacc ggcgcagtcg ccgacgttgt caccgacgtt gtcggcgatc acggccgggt
     1381 tgcggggatc gtcctcgggg atccccgcct cgaccttgcc gaccaggtcg gcgccgacgt
     1441 cggcggcctt ggtgaagatg ccgccgccga gacgggcgaa gaccgagatc agcgaaccac
     1501 cgaagccgag gccgatcagc gcgtcgacgg cctccttgtc ggtgtggtcg gtgatcacca
     1561 ggaagccgaa gtagccggcg atgccgagca gggcgaggcc gacgacgagc agcccggtta
     1621 ccgagccgcc cttgaaggcg acgtcgagcg ccggcggcac gccgccacgg gcggcctcgg
     1681 cgacgcgcga gttggcccgc accgagacgt tcatcccgat gaagccggta gcgccggaaa
     1741 ggacgccgcc gatcacgaag ccgagtgcgg tctcccagtc ctgcaggagc cacagcacga
     1801 tcgccaacgg caccgccact gcggcaatga tcgtgtactg gcggttcagg taagcctgtg
     1861 caccctcctg cacggcgcct gaaatttcct gcattcggtc gtttccgggc gacttcgcga
     1921 gcagtcgctg ggtgacaatg agtccgtatg ccacggcggc gcccgcgcag acaagcgcga
     1981 cgaccacccc gtagtcggtc agaaagctgg tcaagttcga ttcctccggt ggatttagcg
     2041 gcgcgggagt ctataggcga agacggcgtc cgcgcccgct ccctgcgcac taaagcggcg
     2101 ttttgtaaaa agcgacaggt ctaggccggt ctggctggct ggccgactcc ggcgtaggtg
     2161 cgctgcgtct ccgccaccgc ctcgcggtag atctcctcga tcgaggaccc tccggcgagc
     2221 gcctcacggg cccgctgagc gccgtttcgc tccggcagct gcgggtcgat tccaagctgt
     2281 gagcgggctg gagcggtcca ggcaaggatc gagtcgagca gcgcccgcgt ctcgatctcc
     2341 tcgcctttac ggaagtcgat catcttgccg tcggcgccgt aacggatcgc gcgccagagg
     2401 ttctcctcga tctcgcggtc ctggagcggt cccccccgtc cgtcgtagcc gagctcgtcg
     2461 tagtcgagcg ccgtctgcgc gatgcaggcg gcgatcagcc cggccagggc gaaaccctcg
     2521 tccccccggg tctgcgcgtc gcagatccgc acctcaaccg tgccgaaact gtgatgcggg
     2581 cgcacgctcc accacagctg cgtcgactcg accacggtgt tggtccgctg cagtgtggcg
     2641 atgaagtccg cgtagctggc ccagtcgacg aacggcgagg gcacgccgca gcgcgggaac
     2701 gtgcgcgtga agatctccgt gcgcacggtg tgcaggccgc tgtcgcggcg gtcgaggaag
     2761 ggcgagttgg cggagaccgc gagcagcagc ggcagcagct cgcgcagcca gtcgcagacc
     2821 gcgatcgcgc ggtcggcgcc gcggatgccc atgtgcaggt gcaggctcca ggtgttgttg
     2881 cgctgcgcga cccagccgag ttcttcgcgc aggcggttgt agtggggcgt gtcgatgatc
     2941 cgctgatcga ggtagttggc ccaggggtgg gtcccggtcg cgcccagcgc caaacccaac
     3001 tcggcgacca gcgcgaacag ccgcgagcgc cgctcggtct gcagcgcaac agcctcggcg
     3061 aagctctcgg cccgcccaga gcggatctcg acctcggagg cgatcagctc gccggccgcc
     3121 gactcggcca ggacctcgtc cttctgagag gccgcgtaga cgtcctcgaa gcggtgggcc
     3181 agctccagcg tcgccgggtc gaggatcgcg aactcctcct cgagcccgat cgtgaagtcg
     3241 gtcgaggact cgaatctctc gcgggcgagg tcgagatcga gggacaaggt cgggccgcct
     3301 agatggggct caagccaggt agaagtagag cgcgaagagc aggtcgacgg cggggctgga
     3361 ggtgtgcacg gtcacctcgg gcggcacctg cagcagctgc tcggagtagt tgaagatgat
     3421 tttcaccccg gctttgacca gccggtcggc gacgtcctgg gccgcctcgg ccgggaccgc
     3481 gagcacgccg acgacgatct cttcctcggc gacgacctcg tcgagcgcgc cgagctcgcg
     3541 cacctcgatc tcgccgacct ttgtcccaac cacctctggg tcggtgtcga agacggcgac
     3601 tacctggaac ccgtggtcgg cgaagatcgg cgagttggcg atcgcctggc cgaggttgcc
     3661 ggcgccgaac agggcgatgt tgtgctggcc cgaggtctgc aggatcttgc ggatctcgcc
     3721 gagcagcgac tcgatgttgt agccgacccc gcgcttgccg aacttgccga agccggagag
     3781 gtcgcggcgg atctgggtcg ggttgatgtg cgtgtactcg gagagttcct gcgaggagat
     3841 cgtctcgcgg cccatctttt tggcctgggt cagcacctgc aggtagcgcg agagccgggc
     3901 ggcaacgccc agcgagagcc gctcgccgct gaccggcgag gactcggtgg ctccaaactc
     3961 ctcgatcacc tcgaccatgc ccccacagta tcgagccttt cgcgtacggc cgtctcgtcg
     4021 aggatcagct cggaagcgac cattccgtcg atctcgacta ccgggatgcg ctccaagtac
     4081 cggcgcagca gcaggtcctc gctctcgatg tcgatctcgt gcagggagaa gcggtacccc
     4141 tcgccgtgca acgcgatcag ccgctcgatc gcctccgcgc agaggtgaca ttcgggtcgc
     4201 gagtagacga cgacggtggg ctgatcggcg gcgttcatgc ggccggacgc tcggcagcct
     4261 tagcggcgcg cgccacgctg accgcgccct cgccggtcga gaaggcgtcg tagccgaggt
     4321 agtcggggta agagatcggg gtggcgtagc gagcggcgga gccgatcatc gccgcgttgt
     4381 cggtgcagag aggaatcggg accagcttca ggcgcacgcc gtgctgctcg cagagctcgc
     4441 cgaccgcggc gcgcagcggg ccgttggcgg cgaccccgcc gccgagtgcg accgcctccc
     4501 actccccgcc cttcagcgcc cgccgcagct tcgcgaccag ctgaccgacc accgcggcct
     4561 ggaaggacgc ggcgaggtcg gcgcggcgct cctccacccc ctccggcccg aggtcgcggc
     4621 agaggtagac gagcgcggtc ttcaggccac tgaagctgaa gtcgaggccg gggtcgcggc
     4681 tcatcgcgac cggcatctcg aacgcctccg ggtcgccccc ctcggcctcc ttctggatcg
     4741 ccggcccgcc cgggtagccg agaccgagca ggcgcgcgga cttgtcgaag gcctcgccgg
     4801 cggcgtcgtc gagggtctgg ccgagcagct cgtagccctg gtggtcgcgg accgcggcga
     4861 gcagcgtgtg gccaccgctg gcgaccaggc aaaggaacgg cggctcgagc ggctcgggct
     4921 cgaggaagtt ggcggcgacg tggccctgca ggtggtcgac cgggatcagc ggcaggcggc
     4981 gcggcgcggc gatcgccttg gccgtgctca ccccgaccag gagcgctccg atcaagccgg
     5041 ggccggccgt cacggcaacc gcctcgacct ggtcgaggct cgtgcctgct tcggccagag
     5101 ccgcggtcac caccggcgcc gtcagctcga ggtggtggcg cgaggcgacc tcgggcacaa
     5161 ccccgccgaa atcggcatgg gcagcggcct gtgaggagat cgtgttggcg aggacatggc
     5221 cgccgtcgag cacggcggcg caggtgtcgt cgcaggaggt ctctatggcg aggatgagca
     5281 tggcgattga ggcgctgtgg atctcagtcg agccacatga tcagtgcgtc ctcgccattg
     5341 tcctggtagt agcgcgggcg cacccctgcc gagcggaagc caagccgttc gtacatcgtg
     5401 atcgccggtc gattggagac ccgcacctcg agcgtgaagg gcagcttgcc gccaccctcc
     5461 tccagcagcc gggcaatcaa gctgcgggcg acgccagccc ggcgcctctc cggcgccacg
     5521 gcg
//
LOCUS       DASRIG010000335         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_167678, whole genome shotgun sequence.
ACCESSION   DASRIG010000335 DASRIG010000000
VERSION     DASRIG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167678"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFB52_09085"
     CDS             <1..494
                     /locus_tag="VFB52_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06529.1"
                     /translation="SLLSGLTLDEVRDRVLREIGEGKNRYDREVAAALRLSHAALIDP
                     DRVADVMVSGQSNLVDPSTAGDRERLDRMRGLLRALEDKHLLIRLLDRTRTGDGIQVF
                     LGAETAHSALLDSSVVAMAYGPEDRPIGALAVIGPMRMNYGKVMSVVDFTADVVTQLL
                     SEL"
     gene            597..1235
                     /gene="grpE"
                     /locus_tag="VFB52_09090"
     CDS             597..1235
                     /gene="grpE"
                     /locus_tag="VFB52_09090"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZO06530.1"
                     /translation="MDDPNRELAGAPDGPGDEVMGGDNDGDGDGGIAVEVDTRDIDPT
                     KLMRELEAQLARAEQEKKDNWDKYLRTAADLENYRRRSKRDLDDARAETKTRVLKEML
                     PVVDNLERAVEHSEKGGESAAILEGVKLVLRQFLQAFERLDVTPIEAAGQPFDPNLHE
                     AISQVETDQQPPGTIVSVLQRGYRLGDRLLRPALVVVAKPRSAPAGAGEAQG"
     gene            1269..>2475
                     /gene="dnaK"
                     /locus_tag="VFB52_09095"
     CDS             1269..>2475
                     /gene="dnaK"
                     /locus_tag="VFB52_09095"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZO06531.1"
                     /translation="MSRVVGIDLGTTNSCVAVMDGDAAVVIANSEGSRTTPSVVGFAQ
                     TGERLVGQVARRQALTNPENTIYAVKRLMGRKYDDSEVQRQILTCPYEIVGVANGDAH
                     VDVRGRTYSPPEISAMVLTKMRQTAEDWIGEAVTDAVITVPAYFDEAQRQATKDAGRI
                     AGLDVLRIINEPTAAALAYGHEIEGAHRVAVYDLGGGTFDISILELADGVFRVRSTAG
                     DTFLGGEDFDNAIVEWLLMTFAEANAGMDLRGDRLALQRLKEASERAKVELSSGLVTE
                     INLPFIAAAADGPRHLQMPFERIELERMVENLVQKTLEPCRRALSDAELGAHDIDVVI
                     LVGGQTRMPRIQQLVAEFFGREPSRRVNPDEVVAVGAAIQAGVLTGEVQEVLLLDVTP
                     LSLGVETAGG"
BASE COUNT          380 a          890 c          874 g          331 t
ORIGIN      
        1 actcgctcct gtcggggctc accctcgacg aggtccgcga tcgcgtgctg cgcgagatcg
       61 gcgaggggaa gaaccggtac gaccgcgagg tcgccgccgc gctccgcctg agccacgccg
      121 cgctgatcga tcccgatcgg gtcgccgacg tcatggtgtc cggccagtcc aacctggtcg
      181 atccgtcgac ggcgggcgat cgcgagcgcc tcgatcgcat gcgcggcctc ctgcgcgcgc
      241 tcgaggacaa gcacctcttg atccgcctcc tcgatcggac ccgcaccggc gacggcatcc
      301 aggtcttcct cggcgccgag accgcgcact ccgcgctgct ggactcgtcg gtggtggcga
      361 tggcctacgg gccggaggac cgcccgatcg gcgcgctcgc cgtcatcggc cccatgcgca
      421 tgaactacgg caaggtgatg tcggtggtcg acttcaccgc cgacgtggtg acccagcttc
      481 tgtccgagct ctgaacgacg ccgtcgtccg cggccggcga cctctccatc ggggcaggga
      541 cccggggggt ggcggcgcct cctgatcgtg tatatgttgg ccgaaccatg agtgctgtgg
      601 acgacccgaa ccgagagctt gcgggcgcgc cggacggacc cggggacgag gtcatgggcg
      661 gcgacaacga cggcgatggg gatggcggga tcgccgtcga ggtcgacacc cgcgacatcg
      721 atccgaccaa gctcatgcgg gagctcgagg cccagctcgc ccgcgcggag caggagaaga
      781 aggacaactg ggacaagtac ctgcgcacgg ccgccgacct cgagaactac cggcgccgct
      841 cgaagcgcga tctcgacgac gcccgcgccg agaccaagac ccgggtcctg aaggagatgc
      901 tcccggtggt cgacaacctc gagcgcgccg tcgagcactc cgagaagggc ggcgagagcg
      961 ccgccatcct cgagggcgtg aagctggtcc tgcgccagtt cctgcaggcg ttcgagcggc
     1021 tcgacgtcac gccgatcgag gccgcgggcc agccgttcga cccgaacctg cacgaggcga
     1081 tctcacaggt cgagaccgat cagcagccgc cgggcaccat cgtcagcgtg ctccagcgcg
     1141 gctaccggct gggcgaccgg ctgctccggc cggcgctcgt ggtcgtcgcc aaaccccgat
     1201 cggcgccggc cggcgccggc gaggcccagg gctgaggatg cggtgatcgg catggggggc
     1261 cgccaccggt gagccgggtc gtcgggatcg atctcggcac gaccaactcg tgcgtcgccg
     1321 tcatggacgg cgacgcggcg gtggtcatcg ccaactccga gggctcgcgc acgacgccct
     1381 cggtggtcgg cttcgcccag accggcgagc gcctggtcgg ccaggtcgcg cgccgccagg
     1441 cgctgaccaa ccccgagaac acgatctacg cggtcaagcg cctgatgggg cgcaagtacg
     1501 acgactccga ggtgcagcgg cagatcctga cctgcccgta cgagatcgtc ggcgtcgcca
     1561 acggcgacgc ccacgtcgac gtccgcggcc gcacctactc gccgcccgag atctcggcga
     1621 tggtgctgac caagatgcgg cagaccgccg aggactggat cggcgaggcg gtcaccgacg
     1681 cggtcatcac ggtgccggcc tacttcgacg aggcccagcg ccaggcgacc aaggacgccg
     1741 gccgcatcgc cgggctcgac gtcctccgga tcatcaacga gccgaccgcg gcggcgctcg
     1801 cctacggcca cgagatcgag ggggcgcacc gggtcgcggt ctacgacctc ggcggcggca
     1861 ccttcgacat ctcgatcctc gagctcgccg acggtgtgtt ccgggtccgg tcgaccgccg
     1921 gcgacacctt cctcggcggc gaggacttcg acaacgccat cgtcgagtgg ctgctcatga
     1981 ccttcgccga ggcgaacgcc ggcatggacc tgcgcggcga ccgcctggcg ctgcagcggc
     2041 tcaaggaggc gtcggagcgg gcgaaggtcg agctgtcgag cggcttggtc accgagatca
     2101 acctgccgtt catcgccgcc gccgccgacg ggccgcgcca cctgcagatg ccgttcgagc
     2161 ggatcgagct cgagcggatg gtcgagaacc tggtgcagaa gaccctcgag ccgtgccggc
     2221 gcgccctctc cgacgccgag ctgggcgccc acgacatcga cgtcgtcatc ctggtcggcg
     2281 gccagacccg gatgccgcgc atccagcagc tggtcgccga gttcttcggc cgcgagccga
     2341 gccgccgggt caaccccgac gaggtggtcg cggtcggcgc cgccatccag gccggcgtcc
     2401 ttaccggcga ggtccaggag gtcctgctcc tcgacgtgac gccgctgtcg ctcggcgtcg
     2461 agaccgccgg cggcg
//
LOCUS       DASRIG010000336         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2486986, whole genome shotgun sequence.
ACCESSION   DASRIG010000336 DASRIG010000000
VERSION     DASRIG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486986"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VFB52_09100"
     CDS             <1..1167
                     /locus_tag="VFB52_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO06532.1"
                     /translation="CEAIDFLEYYAAGAVALDAGPELVQLPGERNALHYRPRGVVGVI
                     SPWNFPIAIATGMAAAGLATGNAVCLKPAEQSPGCALALSDALARAGLPPGVFSLLPG
                     EGDVGAALVAHPGVHTIAFTGSGAVGLEILSRAAEPSPGQRHLKRVVAEMGGKNCMII
                     DADADLDDVVPAVLASAFGFAGQKCSATSRLLVHERIAELLTERLVGALDTLLFGQAD
                     NFGTDVPPLIEASSRERIERMLAVAGEEGARIHRVGNIPEGGGYFAAPALVTGAGLDA
                     AVVAEEIFGPVVTIEPFAGIDAACDLVDAQAHALTGGLFSRNPRTIAAVARRTPVGNL
                     YVNRGTTGAMVGRQPFGGNRLSGTGTKAGGPDYLLQFVEPVVVTENTVRHGLAP"
     gene            1433..>2038
                     /locus_tag="VFB52_09105"
     CDS             1433..>2038
                     /locus_tag="VFB52_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO06533.1"
                     /translation="MDVELRNYVGGEWVPADSGRMMEVLDPAQETVIAEVPEGSAADV
                     EAAVTAAKDAFPAWRDATPAERAAKLFQLADLIDANAEELAALESRNVGKPQMVSAEE
                     IPLCSDSLRFLGGAARTLTAPAAGEYAGGHTSMIRREPHGVIGAIAPWNYPLMMAIWK
                     FAPALATGNTVVLKPAEATPLTILRLLRLAEGVLPPGVLNVV"
BASE COUNT          300 a          701 c          722 g          315 t
ORIGIN      
        1 tgcgaggcaa tcgacttcct cgagtactac gcggccggag cggtcgccct cgacgctggg
       61 ccggagctgg ttcagctccc cggcgagcgc aatgcgctgc actaccgccc gcgcggcgtc
      121 gtgggcgtga tatcgccctg gaacttcccg atcgcgatcg ccaccgggat ggcggccgcc
      181 ggcctggcga cgggcaacgc ggtctgcctc aagcccgccg agcagtcgcc cggctgcgcc
      241 ctggctctgt ccgacgcgct cgcgcgcgcc ggactgccgc cgggggtgtt ctcgctgctg
      301 cccggcgagg gcgacgtcgg cgccgcgctg gtcgcccatc ccggcgtcca cacgatcgcc
      361 ttcactggct ccggcgccgt cgggctggag atccttagcc gagccgccga gccgagtccg
      421 ggacagcgtc acctgaagcg ggtcgtcgcg gagatgggcg gcaagaactg catgatcatc
      481 gacgctgacg ccgacctcga cgacgtggtc ccggcggtgc tcgcttccgc cttcggcttc
      541 gccggccaga agtgctcggc gacctcgcgg ctcttggtcc acgagcgcat cgctgagctg
      601 ctgaccgagc gcctggtcgg cgcgctcgac acgctcctgt tcggccaggc cgacaacttc
      661 gggaccgacg tgccgccgct gatcgaggcc agctctcggg agcggataga gcggatgctc
      721 gccgtcgccg gcgaggaagg tgcccgcatt catcgggtcg gaaacatccc cgagggcggc
      781 ggatacttcg ccgctccggc gctggtgacc ggtgcggggc ttgacgccgc cgtggtcgct
      841 gaggagatct tcggtccggt ggtcacgatc gagccgttcg ccgggatcga cgcagcctgc
      901 gacctcgtcg acgcgcaggc gcacgcgctg accggcggac tattttcgcg caacccgcgc
      961 acgattgcgg cggtcgcacg ccgcactccg gtcggcaacc tctatgtcaa ccgcggcacc
     1021 accggggcga tggtggggcg ccagccgttc ggcggcaacc gtctctccgg caccgggacc
     1081 aaggccggcg gcccggacta tctgctccag ttcgtcgagc cggtagtagt caccgagaac
     1141 acggtcaggc acggcctggc gccatgattt tggtgcggcc gtgggcgggg cctgaggggt
     1201 acgccgctag atccgttcgg tacgccccgg ggtgcggagg gccgagcgaa gacttcccga
     1261 gtcaccaccg gaaccgcaag ccgaacccgg cggccgcgcc gatcgcggtc gctcctgagt
     1321 cgcttgccca tctcctgggc tacaaccgag tttgtagatt gtgaacaatc tccagtagca
     1381 tcggctggcg accgaaacgg cgacggaggg aaatcgggtg atcgaaaatc tggtggatgt
     1441 cgagctgcgg aactacgttg gaggggagtg ggttccagcc gatagcgggc ggatgatgga
     1501 ggtgctggac ccggcgcagg agacagtgat tgccgaggtt cccgaggggt cggccgccga
     1561 cgtcgaggcc gccgtcaccg ccgccaagga cgccttcccc gcttggcgcg acgcgacccc
     1621 ggccgaacgg gccgcgaagc tttttcagct cgccgatctg atcgacgcca acgccgagga
     1681 gcttgctgcg ctggagagtc gcaacgtcgg caagccgcag atggtctcgg ccgaggagat
     1741 cccgctctgc tcggacagcc tgcgcttcct cggcggcgcg gcccgaaccc tgaccgcgcc
     1801 ggccgcagga gagtacgcgg gaggacacac gtcgatgatc cggcgcgagc cgcacggggt
     1861 gatcggcgcg atcgcgccct ggaactatcc cctgatgatg gcaatctgga agttcgctcc
     1921 agcgctcgct accggcaaca ccgtggtgct gaagccggcc gaggcgacgc cgctcaccat
     1981 cctgcgcctg ctgcggttgg ccgagggcgt gctccctccc ggcgtgttga acgtggtc
//
LOCUS       DASRIG010000337         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1980594, whole genome shotgun sequence.
ACCESSION   DASRIG010000337 DASRIG010000000
VERSION     DASRIG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980594"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFB52_09110"
     CDS             <1..1291
                     /locus_tag="VFB52_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZO06534.1"
                     /translation="GRSSRELRRSKDEDEEFSWSPDRHRTIDRDEVLELVPALAPRDP
                     QSAYMFYDCQTDDVRLVLTVLGEAERFGALSLNGANVTELINDGGNKATGVAFVEADS
                     GERIEVGADHVVNATGVWADEIRPEVVEEEDVPRIAPSRGTHLVLDQADLPMGKAACI
                     VPAGEGRMIFSLPWYGRTLIGTTDNDFDGDITHPRPGADDVEYLLDAVNEFFGTRLGA
                     GDLVGAYAGVRPLISSGDPRKSVDISRKAELYETSSGMLTITGGKLTTWRRMAKQTVD
                     RLVLREGREAPCHTAEIPLGMPARPDDLAAPDGVGEEATAQLAFRYGHAAKRVLDLAR
                     EDPALAAPIAPDRPDLLAEVVLAARHEQARSVADVLLRRTRLGILAAPSLRTAEDVRP
                     VAEQLGAELGWDEARVGREAEAWVEASALEGIDPARP"
     gene            1339..2292
                     /locus_tag="VFB52_09115"
     CDS             1339..2292
                     /locus_tag="VFB52_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HZO06535.1"
                     /translation="MTKAELESKHLSDLHALASEAGVERFRMLTRSELIERLSDGESG
                     GGDNGGGSSRTREPRGDREPRGDREPRGDREPRDRESGDRPRRRRRRRSGSGDALGDE
                     AIGAAPREAESADEDSDEVSGHGDDLGQEALRGAPAAPAPPAPAAAAPSEPERPKRRR
                     RRRRFGRRSKEGVTIQDLLLPPEAGRQAVLAAETREACTALLRGVAGDLAADSKGPDP
                     VVLLIDPTPEELADWKREAPQAEIVAAGQARHANDALAQAAARAGGGDDVILLVDSLT
                     RLATDFGDTDTAKEFFDAGRTQGSAGGGSLTVVAAIERSAA"
     gene            complement(2317..2784)
                     /locus_tag="VFB52_09120"
     CDS             complement(2317..2784)
                     /locus_tag="VFB52_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06536.1"
                     /translation="MDKKTTRRGNYESGSDYVLEYGELRFAFNEEDFGQRVEQAAVKL
                     AFVDQGLSPEELEDLLDLAVSGEIAEPSSRLGEHINENWEDLVGPANRSLVHWIRRLV
                     FRGAWLDQRVKEGELDIVFDERTQTFGYVQPDRSDELIELSKEPSWRRIAYRR"
BASE COUNT          429 a         1089 c         1023 g          405 t
ORIGIN      
        1 cggccgctcc agccgcgagc tgcgcagatc caaggacgag gacgaggagt tctcctggtc
       61 gccggatcgc catcgcacga tcgaccgcga cgaggtgctc gagctggtcc cggcgctcgc
      121 cccccgcgac ccccagagcg cctacatgtt ctacgactgc cagaccgacg acgtgcgcct
      181 ggtgctgacc gtgctcggcg aggccgaacg cttcggcgcg ctctccctga acggggcgaa
      241 cgtgaccgag ctgatcaacg acggcggcaa caaggcgacc ggggtcgcct tcgtcgaggc
      301 cgactcgggt gagcggatcg aggtcggcgc cgaccacgtg gtcaacgcga ccggcgtctg
      361 ggccgacgag atccgcccgg aggtggtcga ggaggaggac gtgccgcgga tcgcgccaag
      421 ccgcggcacc cacctggtgc tcgaccaggc cgatctgccg atgggcaagg ccgcttgcat
      481 cgtccccgcg ggcgaaggcc ggatgatctt ctcgctgccc tggtacgggc gcaccctgat
      541 cggcaccacc gacaacgact tcgacggcga catcacacac ccgcggcccg gtgccgacga
      601 cgtcgagtac ctgctcgacg cggtcaacga gttcttcggc acccgcctcg gcgccggcga
      661 cctggtcggc gcctacgccg gcgtgcgccc gctgatctcc agcggcgacc cgcgcaagtc
      721 ggtcgacatc tcgcgcaagg cggagctgta cgagacctcc tcggggatgc tgacgatcac
      781 cggcggcaag ctcaccacct ggcggcggat ggcgaagcag accgtcgacc ggctggtcct
      841 gcgcgagggt cgcgaggccc cctgtcacac cgccgagatc ccgctcggga tgccggcccg
      901 ccccgatgac ctcgccgcgc ccgacggggt gggggaggag gcgaccgcgc agctcgcctt
      961 ccgctacggg cacgccgcca agcgcgtgct cgacctcgcc cgcgaggacc cggcgctggc
     1021 cgccccgatc gcccccgacc gccccgacct gctggccgag gtcgtgctcg ccgcccgcca
     1081 cgagcaggcg cgcagcgtcg ccgacgtgct gctgcgccgt acccggctgg ggattctcgc
     1141 cgcgccctcg ctgcgcacgg ccgaggacgt gcgcccggtc gccgagcagc tcggcgccga
     1201 gctgggctgg gacgaggcgc gggtcggccg cgaggccgag gcctgggtcg aagcctccgc
     1261 attagaagga atcgaccccg cgcgcccctg agcgcggccc gcaagtcccc cgcaactgcg
     1321 gtggaataac ctgggtcgat gaccaaggcc gagctggaat ccaagcacct gtccgacctg
     1381 cacgccctcg cctccgaggc gggcgtcgag cgcttccgga tgctcacccg cagcgagctg
     1441 atcgagcgtc tcagcgacgg cgagagcggt ggcggcgaca acggtggtgg ctcgtcccgt
     1501 acgcgcgagc cgcgcggcga tcgcgagccg cgtggagacc gtgagccgcg tggagaccgt
     1561 gagccgcgtg atcgcgagtc cggcgatcgc cccaggcgtc gccgtcggcg ccgctccggc
     1621 agcggcgacg cgctcggcga cgaagcgatc ggcgccgccc cgcgcgaggc cgagagcgcc
     1681 gacgaggact ccgacgaagt ttcgggtcac ggcgacgacc tcggccagga ggccctccgc
     1741 ggtgcccctg cggcgccggc cccgccggcc cccgcagccg ctgcgcccag cgagcccgag
     1801 cgccccaaac gccgccgtcg gcgtcgccgc ttcggtcgcc gcagcaagga gggcgtgacg
     1861 attcaggacc tgctgctgcc gcccgaggcc ggccgccagg ccgtgctcgc ggccgagacc
     1921 cgcgaggcct gcacggcgct gctgcgcggt gtcgccggtg acctcgccgc cgactccaag
     1981 ggcccggacc cggttgtcct gttgatcgac ccgacccccg aggaactcgc cgactggaag
     2041 cgcgaggccc cgcaggcgga gatcgtcgcc gccggccagg cccgtcacgc caacgacgcc
     2101 ctcgcccagg ccgccgcccg cgccggcggg ggggacgacg tgatcctgct ggtcgactcc
     2161 ctgacccgtc tcgccaccga cttcggcgac accgacaccg ccaaggagtt cttcgacgcc
     2221 ggccggaccc agggttcagc cggcggtggt tcgttgaccg tcgtcgcggc tatcgagcgt
     2281 tccgccgctt agcggctaga agagaggctt accgctttag cggcggtagg cgatgcggcg
     2341 ccacgacggc tctttcgaca gctcaatcag ctcatctgag cggtccggct gcacgtaacc
     2401 gaaggtctgg gtgcgctcgt cgaagacgat gtcgagctcg ccctccttga cccgctggtc
     2461 gagccaggcg ccgcggaaaa cgaggcggcg gatccagtgg acgaggctgc ggttggcggg
     2521 accgacgagg tcttcccagt tttcgttgat gtgctcgccg agacgggagc tgggttcggc
     2581 gatctcgccg ctgaccgcga ggtccagtag gtcctcgagc tcctccgggc tcaacccctg
     2641 atcgacaaac gcaagcttga ccgccgcctg ctcgacccgc tgaccgaagt cctcctcgtt
     2701 gaaagcgaac cggagctccc cgtactcaag tacgtaatcg gagccagact cgtagttccc
     2761 tcggcgggtc gttttcttgt ccatgaagtt gaagccctct gcctccgcga ctaccgcgtc
     2821 agcaggtgca tgatcttgcc gatcatcgta gtcgcgtgcc cgctcgggcc ggtgcctgaa
     2881 cgggcctttg cacccctacc ggctttcggg gagggggaga gtttccggcg ccgccgtctt
     2941 ggctcg
//
LOCUS       DASRIG010000338         7732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1720513, whole genome shotgun sequence.
ACCESSION   DASRIG010000338 DASRIG010000000
VERSION     DASRIG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720513"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /gene="nuoG"
                     /locus_tag="VFB52_09125"
     CDS             complement(<1..2132)
                     /gene="nuoG"
                     /locus_tag="VFB52_09125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZO06537.1"
                     /translation="MSPEPKEVTLVIDGREVVAQEGEMLHDAARKGDVEIPVFCYEPK
                     LGDPVGACRMCLVEIEGIPKLQTSCSTPVRDGMVVHTRTDQVKHAQNAVVEFLLVNHP
                     LDCPVCDKGGECPLQDVTQGWGPGKSRVIDEKRHFEKPIELSPLVAIDRERCILCYRC
                     VRFSQEVSEDSQLQLLERGDRTFVGTFDDRPYVAPFHGNITDICPVGALTSYTYRFRA
                     RPWDIEQAGSVCTLCPGQCNVSFTVRDERVKRVITRDNHDVDDGWLCDRGRYGFEMFD
                     APERIDAPRLKGGEETEFANAIQLAANGLKAAGGAVAALVGDASNEEGYLVQRILREA
                     LGSNDVDSRLSRGLSREAIVALSQPELTAKVREIDDADVILVAGTEPLGTAPILDLRV
                     RKAIRRNGARLVVATERPTALDGGAEAIARYAPGGAAHFFAELAAAVKGADNVATPLA
                     ESLRGAEKVVVLWSGRIAREGDAAGLALLELAEACGIAAHEGAGMIEVPDVSNGRGLR
                     EAGVLPDAGPGLSAGPSGRSTEEIRAALISGELKGLVLFGVDPLRDFPDTPAWEEALT
                     AADFVVSFSMHENASAAKANVVFPLETHAEKDGTVTHPDGRLQRVRPSAGRPGIVQNN
                     LMVLAELAGALGLESNPYSQPLAFAALTEAVPFYAAITEEKIGGQGIRWQETEGASAM
                     PAGSGEGIQVAVYRPDESASDGLTLGTYR"
     gene            complement(2129..3445)
                     /gene="nuoF"
                     /locus_tag="VFB52_09130"
     CDS             complement(2129..3445)
                     /gene="nuoF"
                     /locus_tag="VFB52_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZO06538.1"
                     /translation="MAHDLETRVLLEHAEDPALRTLEGYKRLAGGYATQERAYRSMEQ
                     DEVLKELEDSGLRGRGGAGFSMGKKASFLPRGEMAKYLCCNADESEPGAFKDRELMQR
                     NPHQLIEGIAIAALAADAGHAFIFIRGEYAEQADILDAAVAEAYEAGYLGKDILGCRR
                     DLELVVHRGAGAYICGEETALLDALEGKRGNPRLKPPFPAVQGLYGGPTLINNVETLS
                     NVPHIVRNGAEWFKGFGTEQSPGTKVVSISGGVRRPGNYEISLGIPLRELIYGLAGGP
                     EPGHEVKAFYPGGSSSPVLTPAELDLPYSFEAMAEAGSMLGSGSIIVADDRVSIPHMA
                     LRTARFYHHESCGKCAPCREGTNWTVKMLERVVRGEATPMDLDIISSVQQNIMGHCLC
                     VLGDSMAMPVSAMVNKFRDEFERVIEAAAMPIAVVQGAPETLGAAT"
     gene            complement(3445..4077)
                     /locus_tag="VFB52_09135"
     CDS             complement(3445..4077)
                     /locus_tag="VFB52_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZO06539.1"
                     /translation="MSPVDGPGIFNPNLPVDQAVTEGRVPPRREEGIPELHEVDCPPQ
                     LKEKIEALMARYPDRRSASIPALWAVQELYGWCTPEGIRQAAAVMRFTPADLESVASF
                     YDLLHTEPVGTHRVEVCTNISCWMRGGDELLDAFRAAGDGQDIFVTGFECLGACDIAP
                     MASIDQRYYGPLEAAEAQTAIEQLLAGQEVLPEKALIARPLAADLPGGSS"
     gene            complement(4074..5273)
                     /locus_tag="VFB52_09140"
     CDS             complement(4074..5273)
                     /locus_tag="VFB52_09140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZO06540.1"
                     /translation="MAETTLLTDDRATEIMTVNIGPHHPATHGVLRLIVDLEGEIVRE
                     CKPVIGYVHTGIEKSCEDKSYWKVIPFVERMDYVSYFFQMQAYCGAVEQLIGLEIPPR
                     AKYLRTLFLELNRIHSHLVWLGTTALDLGAISMLWYCFRERDKILDLFEMATGQRMHT
                     RYFQVGGVIEDIPLGFEQKCRKFAEEMPIRVGQYEALLDRNEVFLQRTKGVGIVDRER
                     LLELGVSGPLLRAAGDPWDLRKAFPYLAYPEMDFKIPVGTVGDNYDRYRVRVAEMVES
                     SRIVAQCLDKLPEGPFIAEDRKYVLPPRSELSTSMESLIHHFKLVTEGFRVEPGEYYN
                     AIECPRGELGCYVLADGSSKPARVHFRDPSFVNLQAFRDMAVDTYVADMICSLGMLDP
                     ILGGVDR"
     gene            complement(5266..5784)
                     /locus_tag="VFB52_09145"
     CDS             complement(5266..5784)
                     /locus_tag="VFB52_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZO06541.1"
                     /translation="MPDAPGLELLRGEIERDRPGSVLATSFERGQAALIVDPAQVLAV
                     LGWLRDEPGQAYRFLSSLHVADYLPAEPRFGVHYELLNRDRVERIRVKAMLADPGDGA
                     LPAIDSCTELFPTANFQEREAFDFFGIEFRGHPDLTRILLPDDYVGWPQRRDFPVGGE
                     PVTFTYNERNYG"
     gene            complement(5777..6445)
                     /locus_tag="VFB52_09150"
     CDS             complement(5777..6445)
                     /locus_tag="VFB52_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZO06542.1"
                     /translation="MEVERRSFQARQATARDMLRGDLEGEDLERYVEEKVVTTTFEKM
                     INLARSNSIFPLTFGLACCAIEAPISIVTARYDLARFGAEALRASPRQADLIILSGRV
                     SIKMAPVVRRLYDQMLEPKWAISMGACSSSGGMFANYAVVQGADKFMPIDLHIPGCPP
                     RPEAVMYGFTKLQRKIQGNPDQGWRQRYNAVGTEEWARGGPASQPSAEAAAAYEQARE
                     LSLA"
     gene            complement(6436..6813)
                     /gene="ndhC"
                     /locus_tag="VFB52_09155"
     CDS             complement(6436..6813)
                     /gene="ndhC"
                     /locus_tag="VFB52_09155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZO06543.1"
                     /translation="MLKTYLPILVFAFLGLAVASVFMVVNHLVGPKKPKLATAASRRQ
                     GEPYESGIPVEPLKNVKFGVSFYLLAMLFILFDIEVVFLYPVGVIMKSADSLLVLGEL
                     VTFVVLLLVAFIYVWRKGALDWR"
     gene            6979..7410
                     /locus_tag="VFB52_09160"
     CDS             6979..7410
                     /locus_tag="VFB52_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06544.1"
                     /translation="MVGLHIAIGFCVIALNLAAGAIGGYAWFRSRPSVPFWYLLRLAQ
                     VSVFIQALLGGLLVVTDHEPSDDLHYVYGILPLAISFIAEGARAGAAQRELGETEFES
                     LDGDTQQEVALAIVRREMGIMAVSCGVIFFVALRAAFTSGL"
     gene            complement(7394..>7732)
                     /locus_tag="VFB52_09165"
     CDS             complement(7394..>7732)
                     /locus_tag="VFB52_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HZO06545.1"
                     /translation="FIAEPSIDFGEDAVTERVLWVDFNGVHALSGLLLFGPAFVFALR
                     PNWALFYAWYATVALYATGIWAFFSEQPAYVFTFPNNSTDAIFHMATGTLFLVVAVVQ
                     TGLDRSRARW"
BASE COUNT         1164 a         2524 c         2806 g         1238 t
ORIGIN      
        1 cggtaggtcc ccagcgtgag cccgtcagag gcgctttcgt cggggcggta gacggcgacc
       61 tggatcccct ctccgctccc cgcgggcatc gccgaagcgc cctcggtctc ctgccagcgg
      121 atgccctggc cgccgatctt ctcctcggtg atcgcggcgt agaacggaac cgcctcggtc
      181 agcgcggcga aggcgagcgg ctgcgagtac gggttgctct cgagcccgag cgcccccgcc
      241 agctcggcga ggaccatcag gttgttctgg acgatgcccg ggcggccggc agatgggcgg
      301 acgcgttgga ggcggccgtc gggatgggtg accgtgccgt ctttctcggc gtgggtctcg
      361 agcgggaaga cgacgttggc cttggcggcg ctcgcgttct cgtgcatcga gaaggagacg
      421 acgaagtcgg cggcggtcag cgcctcctcc caggccggag tgtcggggaa gtcgcgcagc
      481 gggtcgacgc caaacaggac caggcccttg agctcaccgg agatcagcgc ggcgcggatc
      541 tcctcggtcg agcgcccgga ggggccggcg ctgaggcccg ggccggcgtc gggcagcacg
      601 ccggcctcgc gcagaccgcg cccgttggag acgtcgggaa cttcgatcat cccggcgccc
      661 tcgtgggccg cgatcccgca ggcctcggcc agctccagca gcgccaggcc ggcggcgtcg
      721 ccctcgcggg cgatccggcc gctccagagc acgacgacct tctcggcccc gcgcaggctc
      781 tcggccagcg gcgtggcgac gttgtcggcg cccttgaccg cggcggccag ctcggcgaag
      841 aagtgggcgg ccccgccggg ggcgtagcgg gcgatcgcct cggcgccgcc gtcgagcgcg
      901 gtcgggcgct cggtcgcgac gaccaggcgg gcgccgttgc ggcggatcgc cttgcgcacc
      961 cgcaggtcga ggatcggggc ggtgccgagc ggctcggtgc cggcgaccag gatcacgtcg
     1021 gcgtcgtcga tctcccgcac cttcgcggtc agctcgggct gcgagagcgc cacgatcgcc
     1081 tcgcgcgaga ggccgcgcga gaggcgcgag tcgacgtcgt tggagccgag cgcctcgcgc
     1141 aggatgcgct ggaccaggta gccctcctcg ttggaggcgt cgccgacgag cgcggcgacc
     1201 gcgccgccgg ccgccttgag gccgttggcg gcgagctgga tcgcgttcgc gaactcggtc
     1261 tcctcgccgc ctttcagccg cggcgcgtcg atccgctcgg gcgcgtcgaa catctcgaac
     1321 ccgtagcggc cgcgatcgca gagccagccg tcgtcgacgt cgtggttgtc gcgggtgatc
     1381 acccgtttga cccgctcgtc gcgaacggtg aagctgacgt tgcactggcc cgggcagagc
     1441 gtgcagaccg agccggcctg ctcgatgtcc cagggccggg cgcggaagcg gtaggtgtag
     1501 gaggtcagcg ccccgaccgg gcagatgtcg gtgatgttgc cgtggaaggg ggcgacgtag
     1561 gggcggtcgt cgaaggtgcc gacgaaggtg cggtcgccgc gctcgagcag ctgcagctgc
     1621 gagtcctcgg agacctcctg gctgaagcgc acgcagcggt aacagaggat gcagcgctcg
     1681 cggtcgatcg cgaccagcgg cgagagttcg atcggcttct cgaagtggcg cttttcgtcg
     1741 atcacccgcg atttgcccgg gccccagccc tgggtcacgt cctgcagcgg gcactcgccg
     1801 cctttgtcgc agacggggca gtcgagcggg tggttgacga gcaggaactc gaccaccgcg
     1861 ttctgggcgt gcttgacctg gtcggtgcgg gtgtggacga ccatgccgtc gcgaaccggg
     1921 gtcgagcagg aggtctgcag tttcgggatc ccctcgatct cgaccaggca catgcggcag
     1981 gcgccgaccg ggtcgccgag tttcggttcg taacagaaga ccgggatctc gacgtcgccc
     2041 ttgcgggcgg cgtcgtggag catctcgccc tcctgggcga cgacctcgcg gccgtcgatc
     2101 accagcgtga cctctttcgg ctccgggctc acgtcgccgc ccccagggtt tcgggagcgc
     2161 cctggacgac ggcgatcggc atcgccgccg cttcgatcac gcgctcgaac tcgtcgcgga
     2221 acttgttgac catcgccgag accggcatcg ccatcgagtc gccgagcacg cagaggcagt
     2281 ggcccatgat gttctgctga accgagctga tgatgtcgag gtccatcggc gtcgcctcgc
     2341 cgcgcaccac ccgctccagc atcttcaccg tccagttggt gccctcgcgg cagggggcgc
     2401 acttgccgca ggactcgtgg tggtagaagc gcgccgtacg cagcgccatg tgcgggatcg
     2461 agacgcggtc gtcggcgacg atgatcgagc cggagccgag catcgagcct gcttccgcca
     2521 tcgcctcgaa gctgtagggg aggtcgagct cggccggggt gagcaccggc gagctggagc
     2581 cgcccgggta gaacgccttc acctcgtggc cgggctcggg gccgccggcg aggccgtaga
     2641 tcagttcgcg cagcgggatc ccgagcgaga tctcgtagtt gccgggacgg cgcacgccgc
     2701 cggagatcga gaccaccttg gtgcccggcg actgctcggt gccaaagcct ttgaaccact
     2761 ccgccccgtt gcggacgatg tgcgggacgt tggagagcgt ctccacgttg ttgatcaggg
     2821 tcgggccgcc gtagagaccc tgcaccgccg ggaacggcgg cttcaggcgc gggttgccgc
     2881 gcttgccctc gagcgcgtcg agcagcgcgg tctcctcgcc gcagatgtag gcgccggcgc
     2941 cgcggtggac gaccagctcg aggtcgcgcc tgcaaccgag gatgtctttg ccgaggtagc
     3001 ccgcctcata cgcttcggcg accgcggcgt cgaggatgtc ggcctgctcg gcgtactcgc
     3061 cgcggatgaa gatgaaggcg tggccggcgt cggcggcgag cgccgcgatc gcgatcccct
     3121 cgatcagctg gtgcgggttg cgctgcatca gctcgcggtc cttgaacgct ccgggctcgg
     3181 actcgtcggc gttgcagcag aggtacttgg ccatttctcc gcgcggcagg aagctcgcct
     3241 ttttgcccat cgagaagccg gcgccgccgc gcccgcgcag gcccgagtct tcgagctctt
     3301 tgagcacctc gtcctgctcc atcgagcggt aggccctttc ctgggtggcg tagccgccgg
     3361 ccaggcgctt gtagccctcg agggtgcgca gcgccgggtc ctcagcgtgc tcgagcagga
     3421 cgcgggtctc gagatcgtgc gccatcagga gctccctccc ggcaggtcgg cggccagcgg
     3481 ccgcgcaatc agcgccttct ccggcaggac ctcctggccg gcgagcagct gctcgatcgc
     3541 ggtctgcgcc tccgccgcct ccaggggacc gtagtagcgc tggtcgatcg aggccatcgg
     3601 ggcgatgtcg caggcgccca ggcactcgaa cccggtgacg aagatgtcct gcccgtcccc
     3661 cgccgccctg aacgcgtcga gcagctcgtc gccgccgcgc atccagcagc tgatgttggt
     3721 gcagacctcg acccggtggg tcccgaccgg ctcggtgtgc aggaggtcgt agaaggaggc
     3781 gaccgactcc aggtcggcgg gggtaaagcg catcaccgcg gcggcttggc ggatcccctc
     3841 gggggtgcac cagccgtaga gctcctgcac cgcccagagc gccgggatcg aggccgagcg
     3901 gcgatccggg tagcgggcca tcagcgcctc gattttctcc ttcagctgcg gcgggcagtc
     3961 gacttcgtgc agctccggga tcccttcctc gcggcgcggc ggcacccggc cctcggtgac
     4021 cgcctggtcg accgggaggt tcgggttgaa gatgccgggt ccgtcgacgg ggctcaccgg
     4081 tcgactcctc cgaggatcgg gtcgagcatg ccgagactgc agatcatgtc ggcgacgtag
     4141 gtgtcgaccg ccatgtcgcg gaaggcctgc aggttgacga acgaggggtc gcggaagtgg
     4201 acgcgggcgg gcttcgagga gccatcggcg agcacgtaac agccgagctc cccgcgcggg
     4261 cactcgatcg cgttgtagta ctcgccgggc tcgacccgga agccctcggt gacgagcttg
     4321 aagtggtgga tcagcgactc catcgaggtc gagagctccg accgtggcgg cagcacgtac
     4381 ttgcggtcct cggcgatgaa cgggccctcg ggaagcttgt cgaggcactg ggcgacgatc
     4441 cggctcgact cgaccatctc ggcgacccgc acccggtagc ggtcgtagtt gtcgccgacc
     4501 gtgccgaccg ggatcttgaa gtccatctcc gggtaggcga ggtaggggaa ggctttgcgc
     4561 aggtcccagg ggtcgccggc ggcgcgcagc aacgggccgc tgacgccgag ctcaagcagg
     4621 cgctcgcggt cgacgatccc gacccctttg gtgcgctgca ggaagacctc gttgcggtcg
     4681 agcagggctt cgtactggcc gacccggatc ggcatttcct cggcgaactt gcggcacttc
     4741 tgctcgaagc cgagcgggat gtcctcgatc acgccgccga cctggaagta gcgcgtgtgc
     4801 atgcgctggc cggtcgccat ttcgaacagg tcgaggatct tgtcgcgctc gcggaagcag
     4861 taccagagca tcgagatcgc gccgaggtcg agcgcggtgg tgccgagcca gaccaggtgc
     4921 gagtggatcc ggttgagctc gaggaagagg gtgcgcaggt atttggcgcg gggcgggatc
     4981 tcgagcccga tcagctgctc gaccgcgccg cagtaggcct gcatctggaa gaagtaggag
     5041 acgtagtcca tccgctcgac gaaggggatg accttccagt agctcttgtc ctcgcaggac
     5101 ttttcgatcc cggtatggac gtagccgatc accggcttgc actcgcgcac gatctcgcct
     5161 tcgaggtcga caatcaggcg cagcacgccg tgcgtggccg ggtggtgcgg accgatgttg
     5221 acggtcatga tctccgtcgc gcggtcgtcg gtgagcagcg tcgtctcagc catagttgcg
     5281 ttcgttgtag gtgaaggtga ccggctcgcc gccgaccggg aagtcgcgcc gctgcggcca
     5341 gccgacgtag tcgtcgggca gcaggatccg ggtcaggtcc gggtggccgc ggaactcgat
     5401 cccgaagaag tcgaaggctt cgcgctcctg gaagttggcg gtcgggaaca gctcggtgca
     5461 ggagtcgatc gcgggcagcg cgccgtcgcc ggggtcggcc agcatcgctt tgacgcggat
     5521 ccgctcgacc cggtcgcggt tcagcagctc gtagtggacg ccgaagcgcg gctccgccgg
     5581 caggtagtcg gcgacgtgca ggctggagag gaagcggtag gcctggccgg gctcgtcgcg
     5641 cagccagccg agcacggcga gcacctgggc ggggtcgacg atcagcgcgg cttggccgcg
     5701 ctcgaaggag gtggcgagga ccgatccggg gcggtcgcgc tcgatttcac cccgcagcag
     5761 ctccagtccg ggggcgtcag gcaagggaca gctcccgcgc ctgttcgtag gccgccgcgg
     5821 cctcggcgct cggctggctg gccgggccgc cgcgcgccca ctcctcggtg ccgaccgcgt
     5881 tgtagcgctg acgccagccc tgatcgggat tgccctggat cttgcgctgc agcttggtga
     5941 agccgtacat cacggcctcc ggccgcggcg ggcagccggg gatgtggagg tcgatcggca
     6001 tgaacttgtc ggcgccctgg accacggcgt agttggcgaa catgcccccg gaggaggagc
     6061 aggcgcccat cgagatcgcc cacttcggct ccagcatctg gtcgtagaga cggcggacga
     6121 caggcgccat cttgatcgag acccggcccg agaggatgat caggtcggcc tggcgcggcg
     6181 aggcgcgcag cgcctcggca ccgaagcggg cgaggtcgta gcgggcggtg acgatcgaga
     6241 tcggcgcctc gatcgcgcag caggcgaggc cgaaggtgag cgggaagatc gagttcgacc
     6301 gcgcgaggtt gatcattttc tcgaacgtgg tcgtgacgac tttttcttcc acgtagcgct
     6361 cgaggtcctc accttcgaga tcgccacgca gcatgtcgcg ggcagtggcc tgacgggcct
     6421 ggaagctgcg gcgctctacc tccaatcgag tgcccccttt cgccatacgt agatgaacgc
     6481 gaccagaagc aggacgacga aggtgaccag ctcgccgagc accagcaggc tgtcggcgct
     6541 cttcatgatc accccgaccg ggtagaggaa gaccacctcg atgtcgaaga ggatgaagag
     6601 catcgccagc aggtagaagc tgacgccgaa tttgacgttc ttcagcggct cgaccgggat
     6661 ccccgactcg tagggctcgc cctggcggcg gctcgccgcg gtcgccagct tcggcttctt
     6721 cgggccgacc aggtggttta cgaccatgaa gacgctcgcc acggccaggc cgaggaaggc
     6781 aaagacgaga atcgggagat aggtcttgag caccggggaa ttttgggggg ttcgcgggcc
     6841 aaactggcca cccggctatc tcagcctgga accagcaccc gtagacgctg ttatacaccc
     6901 agcttgtgac acattgctac atagtgatat ttcgcatcag gaagcggaat cccgaccgtc
     6961 tgaggcggat ttaggtcgat ggtcggcctg cacatagcca ttggcttctg cgtgatcgcg
     7021 ctcaacctgg ccgcgggagc gatcggcggc tacgcctggt ttcgcagccg accctcggtt
     7081 ccgttctggt acctgctgcg cctggcccag gtctctgtct tcatccaggc cctgctcggc
     7141 gggctacttg tcgtcaccga tcacgaaccg agcgacgatc tccactacgt ctacgggatc
     7201 ctgccgctgg cgatctcgtt catcgccgag ggcgcccgcg ccggcgccgc ccagcgcgag
     7261 ctcggcgaga ccgagttcga gtcgctcgac ggcgacaccc agcaagaggt cgcgctggcg
     7321 atcgtgcgcc gcgagatggg gatcatggcc gtcagctgcg gcgtgatctt cttcgtcgcc
     7381 ctgcgcgcgg cgttcaccag cgggctctag atcggtcgag ccccgtctgg acgacggcga
     7441 cgaccaggaa cagggtgccg gtggccatgt ggaagatcgc gtcggtgctg ttgttgggga
     7501 aggtgaagac gtaggccggt tgctcgctga agaaagccca gatgccggtc gcgtagaggg
     7561 cgacggtcgc gtaccaggcg tagaacagcg cccagttggg gcgcagggcg aagacgaaag
     7621 cggggccgaa gaggagcagg ccggagagcg cgtggacgcc gttgaagtca acccagagga
     7681 cgcgctcggt gaccgcgtcc tcgccgaagt cgatgctggg ctcggcgatg aa
//
LOCUS       DASRIG010000339         6199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2783276, whole genome shotgun sequence.
ACCESSION   DASRIG010000339 DASRIG010000000
VERSION     DASRIG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783276"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..701
                     /locus_tag="VFB52_09170"
     CDS             192..701
                     /locus_tag="VFB52_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06546.1"
                     /translation="MLAPRKSHLLIVLGLVCAFALGAVLPGAFAEDKKAPTAVRNALA
                     ETSKVQGAPNRTLVLSKVTIDPGAQLALHHHLGTQVAHIMKGTLTYTVRKGSAVVSEG
                     EADAEPRVVRTIKAGQTGRIRTGQWIVEQPSDIHEAANRGTVPVVIYLATLLKTGAEP
                     STPVVLAGD"
     gene            complement(712..1020)
                     /locus_tag="VFB52_09175"
     CDS             complement(712..1020)
                     /locus_tag="VFB52_09175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06547.1"
                     /translation="MTAEVRNNPEQNRYELRIEDELAGFVTYRERPGLIALVHTEVDN
                     RLEGQGLASKLVAGALDDARARGLEVLPFCPFANSFIQRHPDEYADLVPEANREQFHL
                     "
     gene            complement(992..1216)
                     /locus_tag="VFB52_09180"
     CDS             complement(992..1216)
                     /locus_tag="VFB52_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06548.1"
                     /translation="MKLNKLFDKAKKTIDDRGGIDSLKGDAQELKKIATGKGSIKDKA
                     KDAAAAIKEPGAAGNDKSEATGDGGSPQQP"
     gene            1281..1353
                     /locus_tag="VFB52_09185"
     tRNA            1281..1353
                     /locus_tag="VFB52_09185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1314..1316,aa:Thr,seq:ggt)
     gene            complement(1453..3441)
                     /locus_tag="VFB52_09190"
     CDS             complement(1453..3441)
                     /locus_tag="VFB52_09190"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZO06549.1"
                     /translation="MPPEEIAPLWEPTQEEVGNAALTRFAEWLARERGVRTDGYRELW
                     QWSVDEPDAFWGALWDYFEIISDAPYEDVLGKREMPGAEWFPGTRLNYAEHVFRGHED
                     AEVALVGASELRELSETTWGELRAEVARVAAGLRELGVGEGDRVVAYLPNIAEAVVGF
                     LATASLGGVWSSASPDFGADSVVDRFAQIEPKVLIACDGYRYGGKDFARLDTVAGLRE
                     RMPSLEHTVLLGYLGGEGELEGAIAWEDLGPAEGAELSFTRVPFSSPLWVLYSSGTTG
                     LPKAIVQSQGGILIEHLKKLILHFDLREGDRFFWFTTTGWMMWNLLVSGLLTKAPIVL
                     YDGNPGHPGLGTLWELAERAGVTCFGTSAAYVAGCMKAGIEPGSEYDLSKIRALGSTG
                     SPLAPEGFDWLYRQLGERLWLFSTSGGTDVCTSFVGGVRTLPVYRGELQAPALGAALA
                     AFDEEGKALIGEVGELVITEPLPSMPVFFWGDKDGSRYHDAYYDMYAGVWRHGDWIEI
                     TPRGTAVIYGRSDSTINRGGIRMGTAEIYRALGDVDEVLDAIVVDLPREGTDGWIALF
                     VVLREGVELGDELRKRVAKEIRSRCSPRHVPDELTVIEEVPRTLSGKVMEVPVKRILM
                     GADPGKVVSKGSMKDPAALDPFIALAATGFAVSSIRSF"
     gene            3464..4639
                     /locus_tag="VFB52_09195"
     CDS             3464..4639
                     /locus_tag="VFB52_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06550.1"
                     /translation="MAGTEIRPEDFLAIDQTLSDQERDIRDTVRAFVRDRVVPNAGEW
                     FDSGEIPIMELAAELGKLNLLGMHLRGYGCAGTSATAYGLACMELEAGDSGIRSLVSV
                     QGSLAMFGIYAWGSEEQKQEWLPRMAAGEVLGCFGLTEPDAGSDPGSMRTRARKDGDD
                     WILHGQKMWITNGSVSEIALVWAHTDEGVRGFLVPKGTPGFTTQDIHGKMSLRASITS
                     ELLLDDVRLPADAVLPGVSSLKGPLSCLNEARFGIVFGATGAARACYESALSYAKERE
                     QFGKPIASFQIQQQKLAQMALEVNRATLLCLHLGRMKDNGTLRSEHVSMGKLANVNAA
                     LDVARSARQVLGANGITLEYPVIRHMNNLETVLTYEGTADVHALVIGNAITGINAFR"
     gene            4674..5285
                     /gene="mftR"
                     /locus_tag="VFB52_09200"
     CDS             4674..5285
                     /gene="mftR"
                     /locus_tag="VFB52_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03968.1"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from
                     the terminal Val-Tyr dipeptide of the precursor peptide
                     MftA, through a radical SAM enzyme-mediated process;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="HZO06551.1"
                     /translation="MAAPSAITSGGRPRSTTRARIAEVALDLFARQGFERTTVDEIAS
                     AAGIGRRTVFRYFPSKNDMVWSDFDRVLERLRAALSAHAQEPMMDALRKAVVESNEYE
                     PGDLPALRMRMTLITRVPALQGHAMLRYGAWRRVVAEFSAARLGCRYDDLLPQTLAHA
                     ALGTSIAAFTRWVRVGDEDLGRCLDQAYRGLADGFDFEAIGHL"
     gene            5355..5864
                     /locus_tag="VFB52_09205"
     CDS             5355..5864
                     /locus_tag="VFB52_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZO06552.1"
                     /translation="MSAKEGFREIDEDRFLSALANFPAGVAVVTSLDPDEQPVGLTIS
                     AFCSVSRTPPLILICVDKGSSTLPAIQHTQKFTVNFLSEGRELVARNFATKDPDKFAD
                     QVWRHSEGLPGGGPILHEDTVAYLVCTVYKAVEAGDHWVFIGEVIEASVEEEARSLLY
                     HRRNFSSIG"
     gene            complement(5848..>6199)
                     /locus_tag="VFB52_09210"
     CDS             complement(5848..>6199)
                     /locus_tag="VFB52_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HZO06553.1"
                     /translation="GNLHYVGRSKEMIKTGGFSVDPVEVENAILDREEVAEAAVLGAA
                     SERWGEEVVAIVVVRPGAELDEAALLAACKQRIASYKVPKRVLTVAELPKNPTGKVER
                     GALRELLAAASRSS"
BASE COUNT          945 a         2179 c         2121 g          954 t
ORIGIN      
        1 agctgcacgt caccctgccc tcggtcgcgg acgagcaggc ggccgagctg gtccgcgaag
       61 cgcacgaggt ctgcccgtac tcgaacgcga cccgcggcaa catcgacgtg gtgctgaccg
      121 ccaacggcaa cccggtctga gcccggctcg gccccggtgt catccgcgct gctgcgtagg
      181 gtgccgccac cgtgctcgcc ccccgcaagt cgcacctgct gatcgtcctc ggcctcgtct
      241 gcgccttcgc tctcggcgcg gtcctgccgg gcgcgttcgc ggaggacaag aaggcgccga
      301 ccgccgtccg caacgcgctc gccgagacca gcaaggtcca gggcgctccg aaccgcaccc
      361 tggtgctcag caaagtgacg atcgacccgg gcgcgcagct ggccctacac caccatctcg
      421 ggacccaggt cgcgcacatc atgaagggca cgctcaccta caccgtgcgc aagggctcgg
      481 cggtggtcag cgaaggcgag gcagacgccg aaccgcgggt cgtgcgcacg atcaaggccg
      541 ggcagacggg caggatccga accggccagt ggatcgtcga gcagccctcc gacatccacg
      601 aagcggccaa ccgcggcacg gtgccggtcg tgatctatct tgcgacgctg ctgaagaccg
      661 gcgcagaacc ttcgacgccg gtcgtcctcg ccggcgactg agcgccgccg cttagaggtg
      721 gaactgctct cggttcgcct ccgggaccag gtccgcgtac tcgtccgggt ggcgctggat
      781 gaaggagttg gcgaaggggc agaagggcag gacttcgagg ccgcgcgcgc gggcgtcgtc
      841 gagcgccccg gcgaccagct tgctggccag cccctggcct tcgagccggt tgtcgacctc
      901 ggtgtggacg agcgcgatca ggcccggccg ctcgcgatag gtgacgaagc cggcgagctc
      961 gtcctcgatc cgcagttcgt agcggttctg ctcagggttg ttgcggactt ccgccgtcac
     1021 cggtcgcctc cgatttgtcg ttgccagccg cgcccggctc cttgatcgcg gccgccgcgt
     1081 ctttggcttt gtccttgatg ctgcctttgc cggtggcgat cttcttcagc tcctgcgcgt
     1141 cccctttgag gctgtcgatc ccgccccggt cgtcgatcgt ctttttcgct ttgtcgaaga
     1201 gcttgttcag tttcattcgc gttcctccgt gaacctttaa gatcgccact cagtgagttt
     1261 ggctccgatc gagccgaccc gcccggatag ctcagttggt agagcacttc catggtaagg
     1321 aaggggtcac gagttcgagt ctcgttccgg gcttcggaga agattgcggc gaatccgaag
     1381 ggggcgccct gacggccggg cggcatctac gtgatgtcgt ctatcgggcc gagtgcttgg
     1441 tggtcgcgag gcttagaacg accgaatgct cgacacggcg aatcccgtcg ccgccagcgc
     1501 gatgaacgga tcgagcgcgg ccgggtcttt catcgagccc ttgctgacca ccttcccggg
     1561 gtcggcgccc atcaggatcc gtttgaccgg gacctccatc accttgcccg agagcgtgcg
     1621 cgggacttct tcgatcacgg tcagctcgtc gggcacgtgg cgcggggagc agcggctgcg
     1681 gatctccttc gcgacccgct tgcgtagctc gtcgcccagc tcgaccccct cgcgcaggac
     1741 cacgaagagg gcgatccagc cgtcggtgcc ctcgcgcggg aggtcgacga cgatcgcgtc
     1801 gagcacctcg tcgacgtcgc cgagcgcgcg gtagatttcc gccgtcccca tgcggatccc
     1861 gccgcggttg atcgtcgagt ccgagcggcc gtagatcacc gccgtgccgc gcggggtgat
     1921 ctcgatccag tcgccgtggc gccagacccc ggcatacatg tcgtagtagg cgtcgtggta
     1981 gcggctgccg tccttatcgc cccagaagaa gaccggcatc gagggcagcg gctcggtgat
     2041 caccagctcg ccgacctcgc cgatcagcgc cttgccctcc tcgtcgaagg ccgcgagcgc
     2101 ggcgccgagc gccggcgcct gcagctcacc gcggtagacc ggcagggtcc ggacgccgcc
     2161 gacgaaagag gtgcagacgt cggtgccgcc gctggtcgag aacagccaga ggcgctcgcc
     2221 gagctggcgg tagagccagt cgaagccctc cggggccagc ggcgagccgg tcgagccgag
     2281 cgcgcggatc ttgctcaggt cgtattcgga gccgggctcg atccccgcct tcatgcagcc
     2341 ggcgacgtag gcggcggagg tgccaaagca ggtgaccccg gcgcgctcgg cgagctccca
     2401 gagggtgccg aggccggggt ggccggggtt gccgtcgtag agcacgatcg gcgccttggt
     2461 cagcaggccg gagaccagca ggttccacat catccagccg gtcgtcgtga accagaagaa
     2521 gcgatcgccc tcgcgcaggt cgaagtggag gatcagcttc ttcaggtgct cgatcaggat
     2581 cccgccctgg ctctgcacga tcgccttcgg cagcccggtc gtcccggagg agtagaggac
     2641 ccagagcggc gaggagaagg ggacgcgggt gaagctcagc tcggcgccct cggccggtcc
     2701 gaggtcctcc caggcgatcg cgccctcgag ctcgccctcg ccgccgaggt agcccagcag
     2761 caccgtgtgc tcgaggctcg gcatccgctc gcgcagcccg gcgacggtgt cgaggcgggc
     2821 gaagtccttg ccgccgtagc ggtagccgtc gcaggcgatc agcaccttcg gctcgatctg
     2881 ggcgaagcgg tcgacgaccg agtcggcgcc gaagtcgggc gaggccgagg accagacgcc
     2941 gcccagcgag gcggtggcga ggaagccgac gaccgcctcg gcgatgttcg gcaggtaggc
     3001 gacgacccgg tcgccctccc ccacgccgag ctcgcgcagc ccggcggcga cccgcgccac
     3061 ctcggcgcgc agctcacccc aggtcgtctc gctcagctcg cgcagctcgg aggcaccgac
     3121 cagcgccacc tcggcgtcct cgtggccgcg gaagacgtgc tcggcgtagt tcaggcgagt
     3181 ccccgggaac cactcggcgc ccggcatctc gcgcttgccg agcacatctt catatggagc
     3241 gtcgctgatg atctcgaagt agtcccagag cgcgccccag aaggcgtcgg gctcgtcgac
     3301 cgaccactgc cagagctcgc ggtagccgtc ggtccggacc ccgcgctcgc gtgccagcca
     3361 ctcggcgaac cgcgtcagcg cggcgttccc gacttcctcc tgggtcggtt cccagagcgg
     3421 tgcgatctcc tccggcggca tgcggcggct actctacgcc ctcgtggcag gtaccgaaat
     3481 acggcccgag gattttctgg ccatcgacca gacgctctcc gaccaggagc gcgacatccg
     3541 cgacaccgtg cgcgctttcg tccgcgaccg ggtcgtcccc aacgccggcg agtggttcga
     3601 ctcgggcgag atcccgatca tggagctggc cgccgaactg ggcaagctga acctgctcgg
     3661 catgcacctg cggggctacg gctgcgccgg caccagcgcg accgcctacg ggctcgcctg
     3721 catggagctg gaggcgggcg actcggggat ccgcagcctc gtctccgtgc aggggtcgct
     3781 ggcgatgttc gggatctacg cctggggctc ggaggagcag aaacaggagt ggctgccgcg
     3841 gatggcggcg ggcgaggtgc tcggctgctt cggcctgacc gagcccgacg ccggatcgga
     3901 ccccggctcg atgcggaccc gggcccgcaa agacggcgac gactggatcc tgcacgggca
     3961 gaagatgtgg atcaccaacg gctcggtgtc ggagatcgcg ctggtctggg cgcacaccga
     4021 cgaaggcgtg cgcggcttcc tcgtcccgaa agggactccc ggtttcacca cccaggacat
     4081 ccacggcaag atgagcctgc gggcctcgat cacctcggag ctgctgctcg acgacgtgcg
     4141 cctgcccgcc gacgcggtcc tgccgggggt gagctcgctg aaaggcccgc tctcctgcct
     4201 caacgaggcg cgcttcggga tcgtcttcgg cgcgaccggc gcggcgcgcg cctgttacga
     4261 atcggcgctc tcctacgcca aggagcgcga acagttcggc aagccgatcg cctccttcca
     4321 gatccagcag cagaagctcg cccagatggc cctggaggtg aaccgcgcca ccctgctctg
     4381 cctccacctc ggccggatga aggacaacgg caccctgcgc agcgagcacg tctcgatggg
     4441 caagctcgcc aacgtcaacg ccgccctcga cgtcgcccgc tccgctcgcc aggtgctcgg
     4501 cgccaacggg atcacgctcg agtatccggt gatccgccac atgaacaacc tcgagaccgt
     4561 gctcacctac gagggcaccg ccgacgtcca cgccctcgtg atcggcaacg cgatcaccgg
     4621 catcaacgcc ttccgctgag caccgcacgc tccctcttat gatgggggcg acgatggcgg
     4681 caccgagtgc cataaccagc ggcggcaggc cacggagcac gacccgggcg cggatcgcgg
     4741 aagtggcgct ggatctgttc gcgcgccagg gattcgagcg caccacggtc gacgagatcg
     4801 cctcggcggc ggggatcggc cggcgcacgg tgtttcgcta cttcccctcc aagaacgaca
     4861 tggtctggtc ggacttcgac cgggtgctgg agcggttgcg ggcggcgctc tcagcgcacg
     4921 cgcaggagcc gatgatggat gcgctgcgca aggccgtggt cgagtcgaac gagtacgagc
     4981 ctggcgacct gccggcgctg cggatgcgga tgacgctgat cacgcgggtg ccggcgctgc
     5041 aggggcacgc gatgctgcgc tacggcgcct ggcgccgggt ggtcgccgaa ttcagcgccg
     5101 cgcggctcgg ctgccgctac gacgacctgc tgccgcagac gctcgcccac gccgcgctcg
     5161 gcacctcgat cgccgccttc acccgctggg tccgggtcgg cgacgaagac ctcgggcgct
     5221 gcctcgacca ggcctaccgc gggctcgcgg acgggttcga cttcgaagcg atcggccacc
     5281 tgtaggcggg cacctatcgc ccgatcggca aaaccttgac gggccgcggg tgcgggccgg
     5341 gataatcggc gtcgatgagc gccaaagagg gtttccgcga gatcgacgag gaccgcttcc
     5401 tgagcgccct ggccaacttc ccggcgggcg tcgccgtggt cacctcgctc gacccggacg
     5461 agcagccggt cgggctgacg atcagcgcct tctgctcggt ctcgcgcacc ccaccgctga
     5521 tcctgatctg cgtcgacaag ggctcgagca cgctgccggc gatccagcac acgcagaagt
     5581 tcaccgtcaa cttcttgagc gagggacgcg agctggtcgc gcggaacttc gccaccaagg
     5641 acccggacaa gttcgccgac caggtctggc gccacagcga ggggctgccg ggcggcggcc
     5701 cgatcctgca cgaggacacc gtcgcctacc tggtctgcac cgtctacaag gcggtcgagg
     5761 ccggcgacca ctgggtcttc atcggcgagg tgatcgaggc cagcgtcgag gaggaggcgc
     5821 gctcgctcct ctaccaccgg cgcaacttca gctcgatcgg ctagccgcgg cgagcagctc
     5881 gcgcagggcg ccgcgttcga ccttgccggt cgggttcttc ggcagctccg cgacggtgag
     5941 cacgcgcttc gggactttgt aggaggcgat tcgctgcttg caggcggcga gcagcgcagc
     6001 ctcgtcgagc tcggcaccgg gacggacgac gacgatcgcg accacttcct cgccccagcg
     6061 ctcgctggcg gcgccgagca ccgcggcctc ggcgacctcc tcgcggtcga ggatcgcgtt
     6121 ctcgacctcg accgggtcga cgctgaagcc gccggtcttg atcatctcct tgctgcgacc
     6181 gacgtagtgc aggttgcca
//
LOCUS       DASRIG010000340         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3277324, whole genome shotgun sequence.
ACCESSION   DASRIG010000340 DASRIG010000000
VERSION     DASRIG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277324"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFB52_09215"
     CDS             complement(<1..732)
                     /locus_tag="VFB52_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06554.1"
                     /translation="MIDPFSLAAAGQVGEDFFKERDASSLCQANDANLFCWDWAKNNI
                     DRYEPFFIEHVELVVISVLIGFVAAFALALLAHRWQRLRLPFLLGTGVLYTIPSVAFF
                     FLLLPLTGRGRDTAIIALSVYTLQIIYRNTMLGLDNVPRSSVDAARGMGMTSRQVLRK
                     VELPLALPEIVAGLIIATVSTVAIATLAALAGGGGLGEVIVGSERGLGISFKTNLIIA
                     GGLTIGLAIVFGLLLMGVQRMLTPWR"
     gene            complement(725..1924)
                     /locus_tag="VFB52_09220"
     CDS             complement(725..1924)
                     /locus_tag="VFB52_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06555.1"
                     /translation="MSPGEAATDISLQTGASAVSYEAATKTYEGSDSPAVDHLTLEVP
                     AGEICVLVGPSGCGKTTAMRMVNRTVEMTSGDIKIGGESVRNRDAARLRREIGYVIQQ
                     IGLFPHRTISENIGAVPALLGWKKERTRARAAELLELIGLDPELGDRYPAQLSGGQQQ
                     RVGVARALAADPLVMLMDEPFGAIDPINRERLQNEFLRLQAEIRKTVLFVTHDIDEAI
                     KMGDRIAVMREGGRVEQYATPAELLMAPANEFVEDFVGADRALKRLALMRVADIDLWE
                     APWATAGQPSAEVRAKLAAPDVEVPYPLLVDAERRPLGWLSERDLAHDTVPATPDSPL
                     GPVLDQADVMRDALSDMLQGGAQYAAVTDHQGRVTGVLSVEIISEFLVSPAAKVEEHG
                     AAERPHD"
     gene            complement(1998..>3092)
                     /locus_tag="VFB52_09225"
     CDS             complement(1998..>3092)
                     /locus_tag="VFB52_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZO06556.1"
                     /translation="LLNGHTHAAMTLFRGSGGDLPLMPWLEERIWPVEAKLSGEDVYW
                     GTRLAAAEMIRGGTTRFWDMYWHPEATARAVADAGIRATIGAPLFDVGRSAEQMRKSA
                     LASLDFLLAAGPLVSPALAPHSIYMVSEDSLHWIAELGAERGVPIHIHLSETQKEVED
                     CIAAHGERPAHYLDRLGALNERTVLAHGVWLDPEELALIAERGATVVTNPVANQKLAV
                     GGVFPYPAARSAGVAVGLGTDGAGSNDSLDLLADLKAFALIQKHAAADPTAIAAADAW
                     AVATGARAPLLGAGSPLTVGAAADFLLLRADAPELGIGDLGANLVYAASGGVVDTTVV
                     AGKVLMRGGEVPDLGEIVARTAERARALGL"
BASE COUNT          434 a         1039 c         1161 g          458 t
ORIGIN      
        1 ccgccacggc gtcagcatcc gctgcacgcc catcaggagg agcccaaaca cgatcgccag
       61 cccgatcgtc agcccgccgg cgatgatcag gttggtcttg aagctgatcc cgaggcctct
      121 ttcactgccg acgatcactt cccccagccc cccgccgccg gcgagtgccg ccagcgtcgc
      181 gatcgccacg gtgctgaccg tggcgatgat caggccggcg acgatctcgg gcagggcgag
      241 cggcagctcg actttgcgca ggacctggcg gctggtcatg cccatgccgc gggcggcgtc
      301 gaccgaggag cgcgggacgt tgtcgagccc gagcatcgtg ttgcggtaga tgatctgcag
      361 cgtgtagacg ctgagcgcga tgatcgcggt gtcgcgcccg cggccggtga gtggcagcag
      421 cagaaagaag aaggcgacgc tggggatcgt gtagagcacg cccgtgccga gcaggaaggg
      481 aagccgcagg cgctgccagc ggtgcgcgag cagggcgagg gcgaaggcgg cgacgaaccc
      541 gatcaggacc gagatcacga ccagttcgac gtgctcgatg aagaacggtt cgtagcggtc
      601 gatgttgttc ttcgcccagt cccagcagaa gaggttcgcg tcgttcgcct ggcacaacga
      661 gctggcgtcg cgttctttga agaagtcttc gccgacctgc ccggccgcgg ccagcgagaa
      721 cgggtcaatc atgcggtcgc tccgcggcgc cgtgttcctc gactttcgcc gccggggaga
      781 cgaggaactc ggagatgatc tcgaccgaga gcacgccggt gacgcggccc tggtgatcgg
      841 tgaccgcggc gtactgggcg ccgccctgga gcatgtcgga gagcgcgtcg cgcatcacgt
      901 cggcctggtc gagcaccggg ccgagcggcg agtcgggggt ggcgggaacg gtgtcgtggg
      961 cgaggtcgcg ctccgacagc cagccgagcg gtcggcgctc ggcgtcgacc agcagcgggt
     1021 agggcacttc gacgtcgggg gcggcgagct tcgcccgcac ctcggcgctc ggctgcccgg
     1081 cggtcgccca gggcgcctcc cagaggtcga tgtcggcgac ccgcatcagc gccagccgct
     1141 tcagcgcccg gtcggcgccg acgaagtcct cgacgaactc gttggcgggc gccatcagca
     1201 gctccgccgg ggtcgcgtac tgctcgaccc ggccgccctc gcgcatcacc gcgatccggt
     1261 cgcccatctt gatcgcctcg tcgatgtcgt gggtgacgaa gaggacggtc ttgcggatct
     1321 cggcctggag gcggaggaac tcgttctgca ggcgctcgcg gttgatcggg tcgatcgcgc
     1381 cgaacggctc gtccatcaac atcaccagcg ggtcggcggc gagcgcgcgg gcgacgccga
     1441 cgcgctgctg ctggccgccg gagagctgcg ccgggtagcg gtcgccgagc tcggggtcga
     1501 gcccgatcag ctccagcagc tcggcggcgc gggcgcgggt gcgctccttc ttccagccga
     1561 gcagggcggg gacggcgccg atgttctcgc tgatcgtgcg gtgcgggaag aggccgatct
     1621 gctggatcac gtagccgatc tcccgccgca gccgggcggc gtcgcggttg cgcaccgact
     1681 cgccgccgat cttgatgtcc ccggaggtca tctcgacggt gcggttgacc attcgcatcg
     1741 cggtcgtctt gccgcagccg gagggcccga cgaggacgca gatctcgccg gccggcacct
     1801 cgagcgtgag gtggtcgacg gcgggggagt cggagccctc gtaggttttc gtcgcggcct
     1861 cgtaactgac cgcggaggcc ccagtttgca gcgatatgtc ggtggcggcc tctcccggcg
     1921 acacgtggat cgagcctata ccccgctgtt acatgcctta cgcaggcttt ctgatgtgtg
     1981 ggttcctaag tcgccatcta cagacctaaa gcgcgggcgc gctcggccgt gcgggcgacg
     2041 atttcgccca gatccggcac ctcgccgccg cgcatcagca ccttgccggc gaccacggtg
     2101 gtgtcgacga ccccgccgct ggcggcgtag acgaggttgg cgccgaggtc gccgatcccg
     2161 agctccggcg cgtcggcgcg cagcagcagg aagtcggcag ccgcgccgac cgtgagcggg
     2221 gagccggcgc cgagcagcgg cgcgcgggcg ccggtcgcga ccgcccaggc gtcggcggcg
     2281 gcgatcgcgg tcgggtcggc ggcggcgtgc ttctggatca gcgcgaaggc cttgaggtcg
     2341 gcgagcaggt cgagcgagtc gttggagccg gccccgtcgg tgccgagccc gaccgcgacc
     2401 ccggcggagc gcgccgccgg gtaggggaag acgccgccga ccgcgagctt ctggttggcg
     2461 accgggttgg tgaccacggt ggcgccgcgc tcggcgatca gcgccagctc ttcggggtcg
     2521 agccagacgc cgtgggcgag caccgtacgc tcgttcaggg cgcccagccg gtcgaggtag
     2581 tgcgcgggcc gttcgccgtg ggcggcgatg cagtcttcga cctctttctg ggtctcggag
     2641 aggtggatgt ggatcggcac cccgcgctcg gcgccgagct cggcgatcca gtgcagcgag
     2701 tcctcgctga ccatgtagat cgagtgcggg gcgagcgcgg gggagaccag cggcccggcc
     2761 gcgagcagga agtcgaggct ggcgagggcc gatttccgca tctgctcggc gctgcggccg
     2821 acgtcgaaca gcggcgcccc gatcgtcgcc cggatgccgg cgtcggcgac cgcccgcgcg
     2881 gttgcctccg ggtgccagta catgtcccag aagcgggtgg tgccgccgcg gatcatctcg
     2941 gcggcggcga gccgggtgcc ccagtagacg tcctcgccgg agagcttcgc ctcgaccggc
     3001 cagatccgtt cctccagcca aggcatcagc ggcaggtcgc cgccgctgcc gcggaacagg
     3061 gtcatcgccg cgtgcgtgtg cccgttcagc ag
//
LOCUS       DASRIG010000341         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_406954, whole genome shotgun sequence.
ACCESSION   DASRIG010000341 DASRIG010000000
VERSION     DASRIG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406954"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFB52_09230"
     CDS             complement(<1..714)
                     /locus_tag="VFB52_09230"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZO06557.1"
                     /translation="MKGKRLRAAAAAALAVAAVSGGLAATGALSAPDPKSTKGQVTVA
                     LGGAKPKNVILLIGDGMGTQEITAARYYQGVEKPLNVDRMKLTGFDTTYSVKPAASAP
                     YLPDFAPDSASTGTMWATGEKTIDERISQGPSSAENVPGVNLKTILEIAQEEGKRTGN
                     VSTAEITDATPAVLASHMSLRGCQGPANMANCPTETKAAGGLGSIAEQEVDHKVDVLL
                     GGGRARFEQTIVGGPTNGQT"
     gene            complement(844..1413)
                     /gene="sepF"
                     /locus_tag="VFB52_09235"
     CDS             complement(844..1413)
                     /gene="sepF"
                     /locus_tag="VFB52_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZO06558.1"
                     /translation="MALRDTWHRALVYFGLAEDHHDYGPEYEDDFEPETGSEEVFEAR
                     RERGDREQASVRRLPTSRRARRDEIDDIFADDEPIAASRTRTLRPVDNGGAGGDIQVH
                     LVIPRNFNDAQQVADQFKRSVPVILNLQTADHDLSKRMIDFCSGLTYALDGGMQRIAE
                     KMFLLTPRNVEVSAEEKARLIDKGFFNQS"
     gene            complement(1482..2117)
                     /locus_tag="VFB52_09240"
     CDS             complement(1482..2117)
                     /locus_tag="VFB52_09240"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZO06559.1"
                     /translation="MAVEPFRDIDPAVVARNLGEVRSVSGEEVEILVAAKYVPLEEMG
                     ALAEAGVALVGENRQQDLAAKHERWGESFTSDFIGNLQSRKVKQVLPLVRLIHSVSTD
                     SVLTQLEKHGTPETEVLIEVNVAGEEEKGGVHPGDLDEFIARCPVRVGGLMTMPPFSQ
                     DPEASRRHFARLAELAGARGLTRLSMGTSQDWRVAVEEGATIIRLGNALFV"
     gene            complement(2117..>2446)
                     /locus_tag="VFB52_09245"
     CDS             complement(2117..>2446)
                     /locus_tag="VFB52_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HZO06560.1"
                     /translation="GLAGGIVAEGAAAVEAESAAIGPGIGACCYEVGDEVLGAFADLG
                     DGIADGRMLDLVEVATRLLRAAGVERVEAAGLCTSCEPELFFSHRRDNGVTGRQGGLA
                     WLDPGAG"
BASE COUNT          339 a          828 c          837 g          442 t
ORIGIN      
        1 ggtctggccg ttggtcggtc cgccgacgat ggtctgctcg aagcgggccc gaccgccacc
       61 gagcagcacg tccaccttgt ggtcgacctc ctgctccgcg atcgagccga ggccaccggc
      121 ggccttggtc tcggtcgggc agttggccat gttggccggg ccctggcagc cgcgcagcga
      181 catgtgcgag gcgagcacgg ccggggtcgc gtcggtgatc tccgcggtcg agacgttgcc
      241 cgtccgcttg ccttcttcct gggcgatctc gaggatcgtt ttgaggttga cgccgggcac
      301 gttctcggcg ctgctcgggc cctgcgagat gcgctcgtcg atcgtcttct caccggtcgc
      361 ccacatcgtc ccggtcgagg ccgagtcggg tgcgaagtca ggcaggtacg gcgcgctggc
      421 cgccggcttg accgagtagg tggtgtcgaa gccggtcagc ttcatccggt cgacgttgag
      481 cggtttctcg acgccctggt agtagcgagc ggcggtgatc tcctgcgtgc ccatgccgtc
      541 gccgatcagc aggatcacgt tcttcggttt cgctccgccg agcgcgaccg ttacctgtcc
      601 tttggtcgat ttcgggtcgg gtgcggagag cgcgccggtt gcggcgagac cgccgctgac
      661 ggctgccacc gcgagcgcgg cggctgccgc ggccctgagg cgtttgcctt tcatcaattt
      721 ccccttctgg tgggtggact ggagggacgg gacttcggcc cgaacctacg aatcacccga
      781 agggggtttg tagccttgct gtagcgaccc tgtgaaagtc gcgttaccga aaactgggac
      841 cggctaggac tggttgaaga agcccttgtc gatcagcctc gccttttcct cggcggagac
      901 ctcgacgttg cgcggcgtca gcaggaacat cttctcggcg atccgctgca tgccaccgtc
      961 cagcgcgtag gtgagcccgg agcagaagtc gatcatccgc ttcgagaggt cgtgatcggc
     1021 ggtctgcagg ttgaggatca ccggcaccga ccgtttgaac tggtcggcga cctgctgggc
     1081 gtcgttgaag tttctgggga tgacgaggtg gacctggatg tcgccgccgg cgccgccgtt
     1141 gtcgaccgga cgcagggtgc gcgtgcggct ggcggcgatc ggctcgtcgt cggcgaagat
     1201 gtcgtcgatc tcgtcgcgac gggcgcggcg cgaggtcggc aggcggcgga ccgaggcctg
     1261 ctcgcgatcg ccgcgttcgc ggcgtgcctc gaagacttcc tcgctgccgg tctccggctc
     1321 gaaatcgtcc tcgtattcgg gaccgtagtc gtgatggtcc tcggcgaggc cgaagtagac
     1381 aagtgctctg tgccaggtat ctctcagtgc catctcacgg gtgattcggt ctagcttgca
     1441 cgactcctct attacccaat tgcagggaga ttagcgccga ttcacacgaa cagggcgttg
     1501 cctaggcgga tgatcgttgc cccctcttcg accgcgaccc gccagtcctg cgaggttccc
     1561 atcgagaggc gtgtcaggcc ccgcgcgccg gcaagctcgg cgaggcgggc gaaatgccgc
     1621 cgcgaggcct ccgggtcctg actgaacggg ggcatcgtca tcaagccgcc gaccctgacc
     1681 gggcagcggg cgatgaactc atcgaggtcg cccgggtgca ccccgccctt ctcctcctcg
     1741 ccggcgacgt tgacctcgat caggacctcg gtctccggcg tcccgtgctt ctccaactgc
     1801 gtgagcaccg agtcggttga gaccgagtgg atcagccgga caagcggcag cacctgcttg
     1861 accttgcggc tctggaggtt gccgatgaag tccgaggtga agctttcgcc ccagcgctcg
     1921 tgcttggcgg cgaggtcctg ctggcggttc tcgccgacca gggcgacgcc ggcctcggcc
     1981 agcgccccca tctcctcgag cggcacgtac ttggcggcga cgaggatttc gacctcctcg
     2041 ccactcaccg accgcacctc cccgaggttg cgggcgacca cggcggggtc gatgtcgcgg
     2101 aacggctcga ccgccatcag ccggcccccg ggtcgagcca ggcaagcccg ccctggcgtc
     2161 cggttacgcc gttgtcgcga cggtgcgaga agaacagctc cggctcgcaa ctggtgcaga
     2221 gcccggcggc ctcgacccgc tcgaccccgg ccgcgcgcag gaggcgcgtc gcgacctcga
     2281 ccaggtcgag catgcggccg tccgcgatcc cgtcgccgag atcggcgaag gcgcccagga
     2341 cctcgtcgcc gacctcgtag cagcaggccc caatcccggg accgatcgcc gcgctctccg
     2401 cttctaccgc ggcggcgcct tcggcgacga tcccgccggc gagccc
//
LOCUS       DASRIG010000342         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1985323, whole genome shotgun sequence.
ACCESSION   DASRIG010000342 DASRIG010000000
VERSION     DASRIG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985323"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFB52_09250"
     CDS             complement(<1..223)
                     /locus_tag="VFB52_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06561.1"
                     /translation="MGALRKVRRGIAYPLRRWPRLTKLTLGGAALLVLFVAFANAYVV
                     LSADQHSTGEVAEVPPAEVAIVPGAGVRWD"
     gene            255..1103
                     /locus_tag="VFB52_09255"
     CDS             255..1103
                     /locus_tag="VFB52_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZO06562.1"
                     /translation="MRLLTAICTAFLIAVLAGCGSSDPTETAPAQSPEIAAKGELRLS
                     EVTIDSEAVGRELDVSVIEPVESTKGKRPLLVFLHGAGGSNESSIGNEAVLNTLAELG
                     DEAPVMAFPDGEESWWHDRASGEWGQYVVEEVIPVLNKRLRTDPERVAIGGISMGGYG
                     AYHLGLAYPGRFCAVGGHSAGLWLDASEEFPGAFDDQAGYEANDVLAAVEADPNAFGP
                     IPVWNDYGDQDWFVAGNAAFVDALEPSTTTALTAHVWPGGHDSAYWDAHFPQYLRFYA
                     RSLAAC"
     gene            complement(1089..2375)
                     /locus_tag="VFB52_09260"
     CDS             complement(1089..2375)
                     /locus_tag="VFB52_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06563.1"
                     /translation="MLARGLSVLGLLIVVLAVLIAPSAAWAAPPPNDNFADREVLSGA
                     LPIEVTRSNVEATKESEESLNLFAAAGHSVWFEWEATSTGWVTIGACEGEEEFMRVLG
                     VYTGTSVNGLTKVAEGNSSEGPACPFTQRQYTFEAQSGTKYEIAVDGNNFTPDPEFTP
                     DTEGVFKLQVEQTPVPTNDNFADAEFVVGKTYEEPDGHRTFWATVKSYNWLATKEVEE
                     PDHEGDPGGASVWFSWTAPESGNARISTCCAGPDLLTVYEGTALPALTPMAELGSPTF
                     GYPVQAGTTYKVAVDGTRSLATNEPRLGSFELQVSMELASGPGYGEEEEPVDKPPVVI
                     APAADTTPPQTTIRKRTVKPGKGKAEFRFASSEAGGSFRCRLDSKPLRTCKSPIKFSN
                     LKAGKHVFKVLAVDAAGNADPSPALARFKIPESAGG"
     gene            2473..2715
                     /locus_tag="VFB52_09265"
     CDS             2473..2715
                     /locus_tag="VFB52_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06564.1"
                     /translation="MNEQEAKDRCAQLAAEHPDRATTNWLPVKQKDGTWAVARIPLPP
                     PAKASGESTKSEHEPLPANPRAENPWINPPPWIGGA"
BASE COUNT          510 a         1052 c          942 g          498 t
ORIGIN      
        1 cgtcccacct gaccccggcg ccgggcacga tcgcgacttc ggccggcggc acttcggcga
       61 cttcaccggt cgagtgctgg tcggcgctga gcactacgta ggcgttggcg aatgccacga
      121 agagcaccag cagcgccgcc ccgccgagcg tgagcttcgt gagccgcggc cagcgccgca
      181 gcgggtaggc gatgcctctg cgtacctttc tcagtgcccc caccggtaaa aaccgtagcg
      241 agttcgaccg actaatgcgc cttctaaccg ccatttgcac tgccttcttg atcgccgtcc
      301 tggccggctg cgggtcaagc gatccgaccg agacggcacc ggcccagtcg cccgaaattg
      361 ctgcaaaagg cgaattgcgg ctgagtgaag tcaccatcga cagcgaggcg gtcggccggg
      421 aactggacgt gagcgtgatc gagcctgtcg agagcacgaa aggaaagcgg ccgctgctgg
      481 tctttctgca cggcgccggc ggctcgaacg aatcctccat cggcaacgaa gccgtgctca
      541 acacccttgc cgaactgggc gatgaagccc ccgtcatggc cttccccgat ggcgaggaaa
      601 gctggtggca tgaccgggcg agcggcgaat ggggccagta cgtagtcgaa gaggtgatcc
      661 ccgtgctgaa caagcgcctg cgcaccgacc cggagcgggt cgcgatcggc ggcatctcga
      721 tgggcggcta cggcgcctac cacctcggcc tcgcctaccc cggccgcttc tgcgccgtcg
      781 gcggccactc agccggcctc tggctcgacg ccagcgaaga attccctggc gccttcgacg
      841 accaggccgg ctatgaagcc aacgacgtgc tcgccgcggt cgaggccgac ccaaacgcct
      901 tcggcccgat cccggtctgg aacgactacg gcgaccagga ctggttcgtc gccggcaacg
      961 ccgccttcgt cgacgcgctc gaaccctcga ccacgaccgc actcaccgcg cacgtctggc
     1021 ccggtggcca cgactccgcc tactgggacg cccacttccc ccagtacctg cgcttctacg
     1081 ccaggtccct agccgcctgc tgactcgggg atcttgaagc gggcgagcgc cgggctcgga
     1141 tcggcgttgc cggccgcgtc gaccgcgagc accttgaaga cgtgcttgcc ggctttcagg
     1201 ttcgagaact tgatcggcga tttgcaggtc cgcagcggct tgctgtcgag gcgacagcgg
     1261 aaacttcccc ccgcctcgct ggaggcgaac cggaactcgg ccttgccctt gcccggtttc
     1321 accgtgcgtt tgcggatcgt cgtctgcggc ggcgtcgtgt ccgccgccgg cgcgatcacg
     1381 acggggggtt tgtcgacggg ttcttcttct tcgccgtagc cggggcccga tgccagctcc
     1441 atggagacct ggagctcaaa cgaacccagc ctcggttcat tcgttgccag gctgcgagtg
     1501 ccgtcaaccg cgaccttgta ggtcgtgcct gcctgaacgg gataaccgaa cgtcggcgac
     1561 cccagttccg ccatcggcgt gagcgccggc agcgcggtcc cctcgtagac cgtgaggagg
     1621 tccggcccgg cgcagcaggt actgatgcga gcgttgcccg actcgggggc agtccaggaa
     1681 aaccaaaccg aggcaccgcc cggatcgccc tcgtggtcgg gctcttcgac ctccttggtg
     1741 gccagccagt tgtaggactt gacggtggcc cagaaggttc ggtggccgtc cggttcttca
     1801 tacgttttac cgacgacgaa ctcggcgtcg gcgaagttgt cgtttgtcgg caccggcgtc
     1861 tgctcgacct gcagcttgaa gaccccttcg gtatccggcg tgaattcagg gtccggagtg
     1921 aagttgttgc cgtcgaccgc gatctcgtat ttggtcccgc tctgcgcctc gaacgtgtac
     1981 tggcgctggg tgaagggaca ggccggtcct tcgctcgagt tgccttcggc gaccttggtc
     2041 agcccgttga cagaggtgcc ggtgtagacc ccgagcaccc gcatgaactc ttcctcgccc
     2101 tcgcaggccc cgatcgtcac ccagccggtg ctcgtcgcct cccactcgaa ccagaccgag
     2161 tgaccggccg cggcgaacag gttgagggac tcttcgctct ctttcgtcgc ttcgacgttc
     2221 gagcgcgtca cctcgatcgg caacgctccg ctgagcacct cgcgatcggc gaagttgtcg
     2281 ttcggcggcg gcgccgccca ggcagcgctg ggcgcgatca gaaccgcgag caccacgatg
     2341 agcagaccga gaacgctgag tccgcgtgcg agcatttcct accccctggc cgagtttgaa
     2401 ggggaccaag ctacaaactc agcgccggcc ccgccactcg aagttcgacc gcgaccctgc
     2461 cagacttgcc gcatgaacga gcaggaagcg aaggaccgct gcgcccaact ggcagccgag
     2521 cacccggatc gcgccaccac caactggctt ccggtcaagc agaaggacgg cacctgggcc
     2581 gtagcccgca tccccctccc gcccccggcc aaagccagtg gcgagagcac caagtccgaa
     2641 cacgaaccgc tcccggccaa cccccgagcc gaaaacccct ggatcaaccc cccaccctgg
     2701 atcggcgggg cctagtcccc cctcaaagcc agggagctac gcgaaatcgc gggatgcgac
     2761 cgtcccgatc gcccacctca gcggccccgt gaggaggggc caggtgcccc tcggagcgcc
     2821 ttgtactttt ttgcttccgc gagtcgaggc gtaaagccgc cgctcactcc acaaaaaagt
     2881 taagcggaga ggggcacctg gcccctcccc gctcggaacc tctagaagtg aacgatcggg
     2941 atgatcagga ttgcgacgat gttcgcgacc ttgatcatcg ggttgatcgc cgggccggcc
     3001 gt
//
LOCUS       DASRIG010000343         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3836723, whole genome shotgun sequence.
ACCESSION   DASRIG010000343 DASRIG010000000
VERSION     DASRIG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836723"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFB52_09270"
     CDS             <1..563
                     /locus_tag="VFB52_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06565.1"
                     /translation="LIVGRRGDGRASRFAGMYTLLRALTASMVPVVVADPEALFEVVP
                     VDAVAAVLAAAAAAAPRGETQIVAAGAVAPRVEDAVELMAAELNEWRGERGLEPFDVP
                     RLVSRESWERFFLPFVRDELSARQRGILDLLSNFEPYLAIREPLEPTHPVPDAAAAIP
                     AAVRYWAETEQRRASLSPRPWTAAAR"
     gene            560..1639
                     /locus_tag="VFB52_09275"
     CDS             560..1639
                     /locus_tag="VFB52_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZO06566.1"
                     /translation="MSGARREQLAERLRGFHAVELEQVLEVIDYLRDGEDSVLVGGSL
                     AYGLGNEKSDLDIVVAGPPGSQSSSRMPLEHFIDTLRVDVWKLRVDELEALFELAQSR
                     LADPAPFAGAFGDVFEQADLKLLHRVAYGVVIDGPQLTPEAGREYRAVAGQLLVREYA
                     ERMRHALFLAQLAQAAGRPLAAAINARFGVEDALQAAIAARGVPFSDNKWLQTRLEGE
                     AGDLREAYRPFMVLPEGGEASATFVAAAVGAAEELAGTALAAGALAADAAWENGGLRL
                     LPAGDLLALLDPEQDVVYELDPEEAEAWRALGEGPSWSCAECSEAQGELCFSLYAEGM
                     LKLTWSRGLPVGELGLRVGELDEVR"
     gene            1626..2135
                     /locus_tag="VFB52_09280"
     CDS             1626..2135
                     /locus_tag="VFB52_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6031 family protein"
                     /protein_id="HZO06567.1"
                     /translation="MKSVDLGARRYIWCRGKAAWLMGPPAAQWLEDLRGALAAGQTGL
                     ARHVSRQLAEDCAVMLALTLTHGRPVPEPNMRAAWALERLAAEPVAADCWELIRGFDE
                     LPADEQLVERCAELQRRARELVGHVPDALSPEGYYPALGLAREWIELVSAVGEGGFLP
                     GEWTRGSDA"
     gene            2128..2571
                     /locus_tag="VFB52_09285"
     CDS             2128..2571
                     /locus_tag="VFB52_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO06568.1"
                     /translation="MPEVDVTLAIEAPAERVWEAVLDIESYPAAMENVRSVRVLGADG
                     EPGQRSAWSIVLKGSILEWEERDLIDEAAMEMRFHQLSGDLSEFDGFWRVRPDGPERS
                     TARFAVTFEIGIPLLAEMLNPVATRSLEENCAEMLRGVERQAVAP"
     gene            2568..3827
                     /locus_tag="VFB52_09290"
     CDS             2568..3827
                     /locus_tag="VFB52_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO06569.1"
                     /translation="MSAAVFEQLRARSAPGTAFAAKLAGGGASEQWAEGARVLLSDGR
                     EAIDFGAYGVTLLGHRHPAVVEAVTAQLRLMPASTRLLANPTTAAFADELGERFGAVG
                     ERVWLGSDGADAVEAAIKLARRASGRMRVLAVEHGFHGKTLGALALTWNPAFREGLEP
                     LLGQVTHVGREDPAAVAREVAVGDVAALIFEPRQGEAGARALDPELLRRWSADARAAG
                     AFVICDEVQAGLRRCGPFSVARAEGVEVDAVLLGKALGGGVMPLSALLARAELYEPLR
                     ADPTFHTATFSGHPLACAAGRAALRVGDRLADEAAAAGARLGAGLELLAAAHPETVVG
                     VHGAGLMWALELAAPALAGSVLIDLARAGLLVSPCLSAPASIRLLPPMVTTEEQLARA
                     LELLDAALAAAAASFAGTASLAGGRSR"
     gene            3824..>4664
                     /locus_tag="VFB52_09295"
     CDS             3824..>4664
                     /locus_tag="VFB52_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO06570.1"
                     /translation="MRALGRFAVRHPRWILAAWVIAAVAALVPAGELTKDVGNGGYAV
                     PGSQSQRVEDLRASQFPAGDGQPLAAVFESRRPAPAQLRQRARRLAAGLGGNPEVHSV
                     GRPMLAPGARTLVLPFTFEGDLAEAQKQVGGISDWVHGVAGGDVVVTGQAAIWEETTA
                     ISKRDLERAERISLPLSLLILVAAFLSLVAAVIPIGLAIVALVVTFAALGLLSQLVDM
                     SVYVTNTAQLLGLGLAIDYSLFIVSRYRERRAEGLDPEAAIVGTVATTGRAVLVSGVT
                     IALG"
BASE COUNT          527 a         1712 c         1785 g          640 t
ORIGIN      
        1 cgctgatcgt cggccgccgc ggcgacggcc gggcgtcccg cttcgccggg atgtacacgc
       61 tgctgcgggc gctgaccgcg agcatggtcc cggtcgtcgt cgccgacccc gaggcgctgt
      121 tcgaggtggt tccggtcgac gcggtggccg cggtgctggc cgcggcggcc gccgccgcgc
      181 cgcgcggcga aacccagatc gtcgctgccg gcgcagtggc gccgcgggta gaggacgcgg
      241 tcgagctgat ggctgccgag ctcaacgagt ggcgcggcga acgcggcctc gagcccttcg
      301 acgtgccgcg gctggtctcg cgcgagagct gggagcgctt cttcctgccg ttcgtccgcg
      361 acgagctctc ggcccgccag cgcggcatcc tcgatctgct ctccaacttc gagccctacc
      421 tggcgatccg cgagccactc gagcccaccc acccggtgcc ggacgccgcc gcggcaatcc
      481 ccgccgcggt tcgctactgg gccgaaaccg agcagcgccg cgcctcgctg tcgccgcgcc
      541 cctggacggc ggccgcacga tgagcggcgc ccgtcgcgag cagctggccg agcgcctgcg
      601 cggcttccac gcggtcgagc tggagcaggt gctcgaggtg atcgactacc tgcgcgacgg
      661 cgaggactcg gtcctggtcg ggggctcgct cgcctacggc ctcggcaacg agaagagcga
      721 cctcgacatc gtcgtcgccg gcccacccgg cagccagtcc tcctcgcgga tgccgctcga
      781 gcacttcatc gacacgctcc gcgtcgacgt ctggaaactg cgcgtcgacg agctcgaggc
      841 gttgttcgag ctggcgcaga gccggctcgc cgacccggct ccgttcgccg gggccttcgg
      901 cgacgtcttt gagcaggccg acctgaagct gctgcaccgg gtcgcctacg gggtcgtcat
      961 cgacgggccg cagctgaccc cggaagccgg ccgcgagtac cgcgcggtcg ccggccagtt
     1021 gctggtccgc gagtacgcgg agcggatgcg ccacgcgctc ttcctcgccc agctggcgca
     1081 ggcggcggga cggccgctgg ccgccgcgat caacgcccgc ttcggggtcg aggacgcgct
     1141 gcaggcggcg atcgccgccc ggggggtccc gttcagcgac aacaagtggc tgcagacccg
     1201 gttggaaggg gaggccggcg acctgcgcga ggcctaccgc ccgttcatgg tcctgcccga
     1261 gggtggcgag gcgagcgcga ccttcgtcgc cgccgcggtc ggcgccgccg aagagctggc
     1321 cgggacggcg ctcgccgctg gcgcactcgc cgccgacgcc gcctgggaga acggcgggct
     1381 gcgcctgctg ccggccggcg acctgctggc cctgctcgac cccgagcagg acgtcgtcta
     1441 cgagctcgat cccgaggagg cggaggcctg gcgggccctg ggcgagggac cgagctggtc
     1501 ctgcgcggag tgcagcgagg cgcagggcga gctctgcttc tcgctctacg ccgaaggaat
     1561 gctgaagctg acctggtcgc gcggcctccc ggtcggcgag ctcggcctgc gggtggggga
     1621 gctcgatgaa gtccgttgac ctcggcgccc gccgctacat ctggtgccgt ggcaaggcgg
     1681 cctggctgat gggccccccg gctgcgcagt ggctggagga tctgcgtggc gcgctcgccg
     1741 ccgggcagac cgggctcgcc cgccacgtca gccgccagct ggccgaggac tgcgcggtga
     1801 tgctggcgct gaccctgacc cacgggcggc cggtgccgga gccgaacatg cgcgccgcct
     1861 gggcgctgga acggctcgcc gccgagccgg tcgcggccga ctgctgggag ctgatccgcg
     1921 gcttcgacga gctgccggcc gacgagcagc tggtcgagcg ctgcgcggag ctgcagcggc
     1981 gggcgcggga gctggttggc cacgtccccg acgcgctctc gccagagggc tactacccgg
     2041 cgctggggct ggcgcgcgaa tggatcgaac tggtttcggc ggtcggcgag ggcggattcc
     2101 tgccgggcga gtggacgcgg ggcagcgatg cctgaggtcg acgtcacact ggcgatcgag
     2161 gcgcccgccg agcgcgtctg ggaggcggtg ctcgacatcg agtcctatcc cgcggcgatg
     2221 gagaacgtgc gctcggtccg cgtcctcggc gccgacggcg agccggggca gcgcagcgcc
     2281 tggtcgatcg tgctcaaggg ctcgatcctc gagtgggagg agcgcgacct gatcgacgag
     2341 gcggcgatgg agatgcgctt ccaccagctc agcggcgacc tctccgagtt cgacggcttc
     2401 tggcgcgtgc gacccgacgg ccccgagcgc tcgaccgcgc gcttcgcggt caccttcgag
     2461 atcggcatcc cgctgctggc ggagatgctg aacccggtcg cgacccgctc gctggaggag
     2521 aactgcgcgg aaatgctgcg cggggtcgag cgccaggcgg ttgcgccgtg agcgccgcgg
     2581 tgttcgagca gttgcgggcc cgcagcgcgc ccggcacggc gttcgccgcg aagctggcgg
     2641 gcggcggggc cagcgagcag tgggccgagg gcgcgcgcgt gctgctctcc gacggccgcg
     2701 aggcgatcga cttcggcgcc tacggcgtca ccctgctcgg tcaccgccac ccggccgtgg
     2761 tcgaggccgt gacggcgcag ctgcggctga tgccggcgag cacgcggctg ctcgccaacc
     2821 cgaccaccgc cgccttcgcc gacgagctgg gcgagcgctt cggcgcggtc ggcgagcggg
     2881 tctggctcgg cagcgatggc gccgacgcgg tcgaggccgc gatcaagctg gcccgccggg
     2941 cgagcggccg gatgcgggtg ctggcggtcg agcacggctt ccacggcaag accctgggag
     3001 cgctggcgct gacctggaac ccggccttcc gcgagggcct cgagccgttg ctcggccagg
     3061 tcacccacgt cggccgtgag gatcccgccg cggtcgcccg cgaggtcgcc gtcggcgacg
     3121 tcgccgcgct gatcttcgag ccacgccagg gcgaggcggg ggcgcgggcg ctcgatcccg
     3181 agctgctgcg ccgctggagc gccgacgcgc gcgccgccgg cgccttcgtg atctgcgacg
     3241 aagtgcaggc tgggctccgc cgctgcgggc ctttctcggt tgcgcgcgcg gagggggtcg
     3301 aggtcgacgc ggtgctgctc ggcaaggcgc tcggcggcgg ggtgatgccg ctctcggcgc
     3361 tgctcgcgcg ggccgagctc tacgagcccc tgcgcgccga cccgaccttc cacaccgcca
     3421 ccttcagcgg ccacccgctc gcctgcgcgg cgggccgcgc ggcgttgcgg gtcggcgacc
     3481 ggctcgccga cgaggcggcg gcggccggcg ccaggctcgg agccggcctc gagctgctcg
     3541 ccgccgccca cccggagacg gtcgtcgggg tgcacggcgc cgggctgatg tgggcgctgg
     3601 agctcgctgc cccggcgctc gccggcagcg tgctgatcga cctcgctcgt gccggcctgc
     3661 tcgtctcacc ctgcctcagc gcccctgcct cgatccgcct gctgccgccg atggtgacca
     3721 ccgaggagca gctggcgcgc gccctcgagc tgctcgacgc ggcgctcgcc gcggcggccg
     3781 caagctttgc cggcaccgcc tcgctggccg gggggcggtc gcggtgaggg cgctcggccg
     3841 cttcgcggtc cgccacccgc gctggatcct cgccgcctgg gtgatcgccg cggttgccgc
     3901 gctggtcccc gccggtgagc tgaccaagga cgtcggcaac ggcggctatg cggttcccgg
     3961 ctcgcagtcc cagcgggtcg aggacctgcg cgcgagccag ttcccggctg gcgacgggca
     4021 gccgctggcg gcggtgttcg agagccggcg cccggccccg gcgcagctgc gccagcgggc
     4081 gcgccggctt gccgccgggc tcggcggcaa cccggaagtg cactcggtcg gccgcccgat
     4141 gctcgccccc ggggcgcgca cgctggtcct cccgttcacc ttcgagggcg acctcgccga
     4201 agcccagaag caggtcggcg ggatctccga ctgggttcac ggggtcgccg gcggcgacgt
     4261 cgtggtcacc ggacaggcgg cgatctggga ggagacgacg gcgatctcca agcgcgacct
     4321 cgagcgcgcc gagcggatct cgctgccgct ctcgctgctg atcctggtcg ccgccttcct
     4381 ctcgctggtc gccgcggtga tcccgatcgg gctcgcgatc gtcgccctcg tcgtcacctt
     4441 cgccgcgctc gggctgctct cgcagctggt cgacatgagc gtctacgtga ccaacaccgc
     4501 gcagctgctc ggcctcggcc tggcgatcga ctactcgctg ttcatcgtca gccgctaccg
     4561 cgagcggcgc gccgaagggc tcgaccccga ggcggcgatc gtcggcacgg tggcgacgac
     4621 cgggcgggcg gtgctggtct ccggggtgac gatcgcgctc ggcc
//
LOCUS       DASRIG010000344         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3836776, whole genome shotgun sequence.
ACCESSION   DASRIG010000344 DASRIG010000000
VERSION     DASRIG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836776"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFB52_09300"
     CDS             <1..620
                     /locus_tag="VFB52_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZO06571.1"
                     /translation="PTNPATGEAEDTDVGYPGADHHIAEQSWPMRAALSVLAFLALVG
                     GLIQVPGVDDVLEKWLDPVFENSPLVDIHPSLGADYVGLTIGGVISIVGIYIAYLIYI
                     KHPGTTARMIERMRPAYTLFVNKWYFDEIIDFLVVRPALAIGRFANRVFERVVVDGLV
                     TGTEGTVRGAGGVVKAVQNGFVRSYALLLVAGFAGLALYFLITSS"
     gene            634..2169
                     /locus_tag="VFB52_09305"
     CDS             634..2169
                     /locus_tag="VFB52_09305"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZO06572.1"
                     /translation="MINALLWAPLAFGIVGLFLPKRAVGWWATLGTLATLVLAAIMVF
                     DFDSGSSGLQHTVDTPWISGLGVDYSLGIDGLNLFLIVLTALLWLGGVAFAAFREWER
                     PRLFYLLMLLAETATLGAFLAQDLLLFVLFFDFMLVPFYFLFGSWGSDREDGPTAAQA
                     TLKMMIYTLIGSLLMLVAAIATAVISADGGHLTFSIAALQQQGLDVDSQRWIFWFFAA
                     AFLVKMPAFLLHGWMPDAYRAAPLPVLAVFSGVLAKVGAYGFLRVVLPVFPDATVEFQ
                     EVILVIALASILYGSVMAFTQTNVRLIAGYSSIAQLGFITAGIFALRADGSDGAILQM
                     VNHGLVVAPIFVIVAILVERTGTEDIREMGGMALRAPVLAVFFLIVALATLAMPGSAN
                     FIGEFYILNGLFQEKEVFAIIAISGVAMSAFYALRLYQQTMHNRKPDGIASREIGFRD
                     AVVVAPLVLAILALALYPQLILKRTAPTVQQTVASVTGGDAPEAELAKNGADDWEPVL
                     IEK"
     gene            2166..>3627
                     /locus_tag="VFB52_09310"
     CDS             2166..>3627
                     /locus_tag="VFB52_09310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZO06573.1"
                     /translation="MNFNEPTIDYAGLSPVIALTVGLCVVLLSSVFKPVKRWAPGLTL
                     IVLATASGVLIWRWGEPADLVAGALRIDDLAISISLIAILTAAFVVVISQRESAAEQA
                     GVGEYHALLIGSVLGMVLLAEAQNLVSFFVAIETLSIPLYVLCATNMRREGSLESGLK
                     YLIVGSVGSATLLYGMAFLYGGTASTDFNQIAAGIAHKDLIGDPLILIGIAMCAVGLA
                     FKTSIAPFHQWTPDVYEGAPTPITGFMAVATKAAAFAVFIRFFDVALGPEVDDWQPAL
                     AVLAAISIIVGNVGALGQDSLKRLLGYSGIAQAGYMLGGIVVATEAGVNAMVFYLAAY
                     AFMNLAAFAVIVARERETPFGDDIRAVRGLGAERPWLAWPLTISMLALAGLPATAGFI
                     GKLYLIEALVEGDYTWLGVFIAVGTMISLGYYLRVVAAMWMRPEREPASGTPAIAGAS
                     PEADPVDPDAGTRWYLVGPAVLAAAATVFFGVIPQPL"
BASE COUNT          466 a         1325 c         1174 g          662 t
ORIGIN      
        1 cgccgaccaa cccggcgacc ggcgaggccg aggacaccga cgtcggctac cccggcgccg
       61 accaccacat cgccgagcag tcctggccga tgcgggcggc gctgagcgtg ctcgccttcc
      121 tcgccctggt cggcggcctg atccaggtcc ccggcgtcga cgacgtgctc gagaagtggc
      181 tcgacccggt cttcgagaac tccccgctcg tcgacatcca cccgagcctc ggcgccgact
      241 acgtcgggct gacgatcggc ggcgtgatct cgatcgtcgg catctacatc gcctacctga
      301 tctacatcaa gcacccgggc acgaccgcgc ggatgatcga gcgcatgcgc ccggcctaca
      361 ccctgttcgt caacaagtgg tacttcgacg agatcatcga cttcctcgtc gtccgcccgg
      421 cgctggcgat cggccgcttc gccaaccggg tcttcgagcg ggtcgtcgtc gacgggctcg
      481 tcaccggcac cgagggcacg gtgcgcgggg ccgggggcgt cgtcaaggcg gttcagaacg
      541 gcttcgtgcg cagctacgcc ctgctgctcg tcgccgggtt cgccggtctc gccctctact
      601 tcctgatcac cagctcatga gcgaggcgac ccgatgatca acgcgctgct ctgggccccg
      661 ctcgccttcg ggatcgtcgg cctcttcctg cccaagcggg cggtcggctg gtgggcgacg
      721 ctcggcaccc tggcgaccct ggtgctggcg gcgatcatgg tcttcgactt cgactccggc
      781 agctccgggc tgcagcacac ggtcgacact ccctggatct ccgggctcgg cgtcgactac
      841 agcctcggga tcgacggcct gaacctcttc ctgatcgtgc tcaccgcgct gctctggctc
      901 ggtggcgtcg ccttcgccgc cttccgcgag tgggagcggc cgcggctctt ctacctgctc
      961 atgctgcttg ccgagacggc gacgctgggg gcgttcctgg cccaggacct gctgctcttc
     1021 gtcctcttct tcgacttcat gctggtcccg ttctacttcc tcttcggcag ctggggcagc
     1081 gaccgcgagg acggcccgac cgccgcccag gcgacgctga agatgatgat ctacacgctg
     1141 atcggctcgc tgctgatgct ggtcgcggcg atcgccaccg cggtcatctc cgccgacggc
     1201 ggccacctca ccttctcgat tgcggcgctc cagcagcagg gcctcgacgt cgacagccag
     1261 cgctggatct tctggttctt cgccgccgcc ttcctggtca agatgcccgc cttcctcctg
     1321 cacgggtgga tgcccgacgc ctaccgggcg gcgccgctgc cggtcctggc ggtcttctcc
     1381 ggcgtgctcg ccaaggtcgg cgcctacggc ttcctgcggg tcgtcctgcc ggtcttcccc
     1441 gatgcgaccg tcgagttcca ggaggtgatc ctggtgatcg cgctggcctc gatcctctac
     1501 ggctcggtga tggccttcac ccagaccaac gtgcggctga tcgccggtta ctcctcgatc
     1561 gcccagctcg gcttcatcac cgccgggatc ttcgccctgc gcgccgacgg ctccgacggg
     1621 gcgatcctgc agatggtcaa ccacggcctc gtcgtcgccc cgatcttcgt gatcgtcgcg
     1681 atcctggtcg agcgcaccgg taccgaggac atccgcgaga tgggcgggat ggcgctgcgc
     1741 gccccggtcc tcgcggtgtt cttcctgatc gtcgccctgg cgaccctggc gatgcccggg
     1801 tcggccaact tcatcggcga gttctacatc ctcaacgggc tcttccagga gaaggaggta
     1861 ttcgcgatca tcgcgatcag cggcgtcgcg atgtcggcct tctacgcgct gcggctctac
     1921 cagcagacga tgcacaacag aaagccggac gggatcgcct cgcgtgagat cggcttccgc
     1981 gacgcggtcg tggtcgcgcc gctggtcctc gcgatcctcg ccctggcgct ctacccgcag
     2041 ctgatcctga agcggaccgc gccgaccgtg cagcagacgg tcgccagcgt gaccggcggc
     2101 gacgcaccgg aagcggagct ggcgaaaaat ggcgccgatg actgggaacc ggtgttgatc
     2161 gaaaaatgaa cttcaacgag ccgaccatcg actacgccgg cctctcgccg gtcattgccc
     2221 tcaccgtcgg gctctgcgtc gtcctgctca gctctgtgtt caagccggtc aagcgctggg
     2281 cgccggggct gaccctgatc gtgctcgcga ccgcctccgg cgtcttgatc tggcgctggg
     2341 gcgagcccgc cgacctggtc gccggggcgc tgcggatcga cgatctggcg atctcgatct
     2401 cgctgatcgc gatcctcacc gccgccttcg tcgtcgtgat ctcgcagcgc gagtcggccg
     2461 ccgagcaggc cggggtgggg gagtaccacg cgctgctgat cggctcggtg ctcgggatgg
     2521 tgctgctggc cgaggcgcag aacctggtct ccttcttcgt cgcgatcgag acgctctcga
     2581 tcccgctcta cgtcctctgc gcgaccaaca tgcgccgcga gggctcgctc gagtcggggc
     2641 tcaagtacct gatcgtcggc tcggtcggct cggcgaccct gctctacggg atggccttcc
     2701 tctacggcgg caccgcctcc accgacttca accagatcgc cgccgggatc gcccacaaag
     2761 acctgatcgg cgacccgctg atcctgatcg ggatcgcgat gtgcgcggtc gggctcgcct
     2821 tcaagacctc gatcgcgccc ttccaccagt ggacgccgga cgtctacgag ggcgcgccga
     2881 cgccgatcac cgggttcatg gcggtggcga ccaaggcggc ggccttcgcc gtcttcatcc
     2941 gcttcttcga cgtcgcgctc ggccccgagg tcgacgactg gcagccggct ctcgccgtgc
     3001 tggcggcgat ctcgatcatc gtcggcaacg tcggcgcgct cggccaggac tcgttgaaac
     3061 gcctgctcgg ctactcgggg atcgcccagg ccggctacat gctcggcggc atcgtcgtcg
     3121 ccaccgaggc gggggtcaac gcgatggtct tctacctcgc cgcctacgcc ttcatgaacc
     3181 tcgccgcctt cgcggtgatc gtcgcgcgcg agcgcgagac cccgtttggc gacgacatcc
     3241 gcgctgtgcg cgggctcggc gccgagcgcc cctggctcgc ctggccgctg acgatctcga
     3301 tgctggccct ggccgggctg ccggcgactg ccggcttcat cggcaagctc tacctgatcg
     3361 aggccctggt cgaaggtgac tacacctggc tcggcgtctt catcgcggtc ggcacgatga
     3421 tctcgctcgg ctactacctg cgggtggtgg cggcgatgtg gatgcggccc gagcgcgagc
     3481 ccgcgagcgg caccccggcg atcgccggcg cctcgcctga ggccgacccg gtcgatccgg
     3541 acgccggcac gcgctggtac ctggtcggcc ctgccgtgct ggcggccgcc gcgaccgtct
     3601 tcttcggcgt gattccccag ccgctcg
//
LOCUS       DASRIG010000345         8166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3033452, whole genome shotgun sequence.
ACCESSION   DASRIG010000345 DASRIG010000000
VERSION     DASRIG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033452"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFB52_09315"
     CDS             <1..981
                     /locus_tag="VFB52_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HZO06574.1"
                     /translation="IVVSRRSPELIKSLFELEVPEIADKLVEIVGVAREPGYRSKIAV
                     VSHADGVDPVGACVGPRGSRVRMVVSELRGEKIDIIPHNDEPARFVAKALSPARVREV
                     IVDDDERQATVIVPDDQLSLAIGRDGQNARLAARLTGWRVDIKSETDFASEEQDIEFE
                     GEEAFDGRCLAVLSNGRRCPNAAIPGSTYCGLPQHQALSRFETSQVAVIAGVSEAEVA
                     ILADPDADQGQVDEIVERATANFVEPVEAEGTPEEELAEAEAAEVDEQAQDALDEVVD
                     EAIAEVETEQAQEDPVEAEIEEEIADEAAVEAEAEATPENGAVTEEAEQA"
     gene            981..3299
                     /gene="infB"
                     /locus_tag="VFB52_09320"
     CDS             981..3299
                     /gene="infB"
                     /locus_tag="VFB52_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZO06575.1"
                     /translation="MAKKRVHEIAKAQGLSSKELLAALKAAGVEAKAAASSVEEADAV
                     KAIAAAKGNGGAEAKAPAKQAQAEAPAKPAQPAAEKPAAKPAPAKPAEEPKAAPAAKA
                     AAPAPKAGNASTKPVRPAGSQGGAGGGAGGPGGAKKRRVVIDSQAARRDQMGGPPPQR
                     PPRRRGGRRRRPLLEEPPMGPATPAEPEATKVNSGATVSEVAESLGLAPAEVIKKLMM
                     MGEMATLTQTLTDESIRAIADEYDRKLEIVSAADEEPEEPEFDDAAEDLTDRPPVVTI
                     MGHVDHGKTSLLDAIRETEVAAGEAGGITQHIGAYQVHHEDKTITFLDTPGHAAFTAM
                     RARGAKVTDVAVVVVAADDGVMPQTKEAIDHARAADVPMLIAVNKIDKEGANPERVRT
                     ELAQEGIQPEEWGGDTVFAEVSAKTHEGLDNLLDMILLVTELEELGANPDAPASGTVI
                     ESQLDPGRGPVVTVLVQRGTLRVGDSLVAGPQWGRVRAMHDFTGARVEEAEPGMPVEV
                     LGFDGVSDAGEFVQAVENDRRARQLAQERADRLKREALARRNARKVSLDEVFARAQQG
                     EIKELNIVLKGDVAGSLEALQDEIAKVPQDRVAINIIHSQTGGINESDVMLASASDAI
                     IIGFNVRPLLDAKRAAEREGVDVRTYSVIYKITEDLRNAMEGLLEALEVEETVGEAEI
                     KQTFKASKVGRIAGCIVTQGKAVRSASVRLIREGTVIWTGELASLRRFKDNVQEVEEG
                     QECGIVLDGYADVKEDDVIEFFQTKQVEQTLE"
     gene            3315..3677
                     /gene="rbfA"
                     /locus_tag="VFB52_09325"
     CDS             3315..3677
                     /gene="rbfA"
                     /locus_tag="VFB52_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZO06576.1"
                     /translation="MAGARMRRINEVLREVVGATIAGDLSDPRIGFVTVTAVETSPDL
                     RSAKVYVSVLGDEEEREATLSALRSSHGVIQSRIAAETRMKRTPTLTFHYDDTIEKGV
                     RISHLLEETADSGEGDER"
     gene            3674..4681
                     /locus_tag="VFB52_09330"
     CDS             3674..4681
                     /locus_tag="VFB52_09330"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZO06577.1"
                     /translation="MSEATGLATSSDIERVGAELRARQRFLLTAHEGPDGDALGSLLG
                     MHHLLTQLGKDSVMFLAAKEFPLPIEYRFLPLEEVFHEAPADMADRTVVFLDCGNIDR
                     MPVDFLTDGDNFTLNIDHHHDNTRFGDVNLVDVEASCTAEIVYDLAKLLGAEITQPMA
                     AALYVGLITDTGKFMYENTNAHTHRVAAELIDAGVDVDDTYRRLYEHVPLEKLRLVAR
                     ALGGIQRHCRDRLAISYITAADYEATGAGEEMTEGVIDHLRSIEGIKVAAVVRDLGNR
                     GRAARKVSLRSSEGDVDVSAIARLNGGGGHKRAAGFSTDLELDDLVTFLCEQVVAQLG
                     E"
     gene            4690..5337
                     /gene="truB"
                     /locus_tag="VFB52_09335"
     CDS             4690..5337
                     /gene="truB"
                     /locus_tag="VFB52_09335"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZO06578.1"
                     /translation="MTETGGAVLLYDKPAGVTSHDIVARVRRERGGKTGHAGTLDPFA
                     TGLLLILRGRATRLQRFLVGLPKTYLATARLGWRSSTGDPDGELTETGAIPDSLELPT
                     GTVRQRVPMTSAVRVGGERLYKKAHRGEVVETPERDVEVHRAELLSAADGRAHYEIEC
                     SAGTYVRTLIETLDDAYCEELRRTAIGPFRVEDAGEELPVERVAELVPAVAEALR"
     gene            5334..6230
                     /locus_tag="VFB52_09340"
     CDS             5334..6230
                     /locus_tag="VFB52_09340"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZO06579.1"
                     /translation="MSIEVTMLADVKRRPRHVAIGTFDGVHLGHQAVIDHADTVLTFD
                     PHPMSVLRPEAAPPLISTFSVRRDVLDGLGVAELVVIPFDKEFSQLGPNEFIEDVLIE
                     RLGAVKVSVGENFHFGAKAEGSPQLLADRTEFETRIVPLIEIDGDTVSSSRIREQIAN
                     GEVAAARRCLGAPFMVEGTVVKGDQRGRELGFPTANIVPDERLVTPGHGVYAAFANGA
                     PAAVNVGVRPTFESELGVLIETYLLDRDEDLYGKTLRVAFVEKLRDEERFEQVDELIE
                     QMKRDVEDAREVCASFTHSGEP"
     gene            6298..7668
                     /locus_tag="VFB52_09345"
     CDS             6298..7668
                     /locus_tag="VFB52_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06580.1"
                     /translation="MPLRAGALAALSTLLAALALAGTGSAAPNPDIAAFRIGHGAWAT
                     GFTLGPDGRFWFVGNTGNTDKAHPEGMVGWFTTDGASDEARLPSHSVPGGGKIADGPD
                     GRLWFTDPVTNQVGVVNAVGLFEQYPLPTPGASPGAIVSGPGDAMWFVEENADKVGRV
                     EMDGDIGETSLPEGAQPTGIVEGSDGALWIVAKGLGKILRMNSNGEVSDEYALPDPDS
                     RPHAIVHGPGGDLWFSEEDGPRIGRINAAGAITEYPIPGENGTRELALGGDGNLWFTA
                     GSAIGSISASGETGEPECVSNGCGYPINALAKGPEGGIWFATDILESGEAGGGTPQLA
                     YLAGGLVGRFLAPELSVRLGRRATRVDDGLTTIAISCHGGTAGEACRGWLRLTTKLRG
                     RKVLLDQHRYRLEPATSRRLPLRLGARGKRTVARAKRIKVQISASLIEGSGARRGFVL
                     QARRRR"
     gene            7749..8018
                     /gene="rpsO"
                     /locus_tag="VFB52_09350"
     CDS             7749..8018
                     /gene="rpsO"
                     /locus_tag="VFB52_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZO06581.1"
                     /translation="MSLTKEKKSELIGKYGRAEGDTGSAEVQIALLTERINDLTGHLR
                     EHSKDHHSRRGLLMLVGKRRRMLRYLERNDLERYRALVAELGLRR"
BASE COUNT         1407 a         2857 c         2796 g         1106 t
ORIGIN      
        1 atcgtcgtct cgcgccgcag cccggagctg atcaagagct tgtttgagct cgaggtgccg
       61 gagatcgccg acaagctggt cgagatcgtc ggcgtcgccc gtgagcccgg ctaccgctcg
      121 aagatcgccg ttgtctccca cgccgatggc gtcgacccgg tcggggcctg cgtcggcccg
      181 cgcggctcgc gcgtgcgcat ggtcgtctcc gagttgcgcg gcgagaagat cgacatcatt
      241 ccccacaacg acgagcccgc ccgcttcgtc gccaaagccc tttcaccggc ccgcgtgcgc
      301 gaggtgatcg tcgacgacga cgagcgccag gcgaccgtga tcgtgcccga cgaccagctc
      361 tcgctggcga tcggccgcga tggccagaac gcccgcctcg ccgcccgcct gaccggctgg
      421 cgcgtcgaca tcaagtcgga gaccgacttc gcctccgagg aacaggacat cgagttcgag
      481 ggcgaggaag ccttcgacgg ccgctgtctg gcggtgctct ccaacggccg ccgctgcccg
      541 aacgcggcga tccccggttc gacctactgc ggcctgcccc agcaccaggc gctctcgcgc
      601 ttcgagacca gccaggtggc ggtgatcgcc ggcgtctccg aggccgaggt tgcaatcctc
      661 gccgaccccg acgccgacca gggccaggtc gacgagatcg tcgagcgcgc cacggccaac
      721 ttcgtcgagc cggtcgaagc ggagggcacg ccggaggagg agctcgccga ggccgaggcc
      781 gccgaggtcg acgagcaggc ccaggacgct ctcgacgagg tcgtcgacga ggcgatcgcc
      841 gaggtcgaga ccgagcaggc ccaggaggat ccggttgagg ccgagatcga ggaggagatc
      901 gccgacgagg ccgccgttga ggcggaagcc gaggccaccc ccgagaacgg cgcggtcacc
      961 gaggaggctg agcaggccta gtggctaaaa aacgcgtcca cgagatcgcg aaagcgcagg
     1021 gcctgagcag caaagagctg ttggccgcgc tgaaagccgc tggcgtcgag gcgaaggcgg
     1081 ccgcctccag tgtcgaggag gccgacgccg tgaaggcgat cgcggccgcg aagggcaacg
     1141 gcggcgccga ggcgaaagcc ccggccaagc aggcgcaggc cgaggctccg gcgaaacccg
     1201 cccagcccgc tgccgagaaa cccgccgcca aaccggcccc ggcgaaaccc gccgaggagc
     1261 cgaaagccgc tccggccgcc aaagctgccg ctccggcgcc gaaagcgggc aatgcttcga
     1321 ccaaaccggt gcgcccggcc ggctcccagg gtggcgcggg cggcggtgcc ggtggccccg
     1381 gcggcgcgaa aaaacgccgc gtggtgatcg attcgcaggc cgcccgtcgc gaccagatgg
     1441 gcggaccgcc gccgcagcgc ccgccacgcc gcaggggtgg gcgacgccgc cggccgctgc
     1501 tcgaggagcc gccgatgggg ccggcgacgc cggccgagcc cgaggcgacc aaggtcaact
     1561 ccggtgccac ggtcagcgag gtcgccgaaa gcctcggtct ggccccggct gaggtgatca
     1621 aaaaactgat gatgatgggc gagatggcga ccttgaccca gacgctcacc gacgagtcga
     1681 tccgggcgat cgccgacgag tacgaccgca agctggagat cgtcagcgcc gccgacgagg
     1741 agcccgagga gcccgagttc gacgacgccg ccgaggacct caccgaccgc ccgccggtgg
     1801 tcacgatcat gggtcacgtc gaccacggca agacctcgct gctcgacgcg atccgcgaga
     1861 ccgaggtcgc cgcgggcgag gccggcggga tcacgcagca catcggcgcc taccaggtcc
     1921 accacgagga caagacgatc accttcctcg acacgcccgg ccacgccgcc ttcacggcga
     1981 tgcgcgcccg cggcgccaag gtcaccgacg tcgccgtcgt ggtcgtcgct gccgacgatg
     2041 gcgtgatgcc gcagaccaag gaggcgatcg accacgcgcg cgccgccgac gtgccgatgc
     2101 tgatcgcggt caacaagatc gacaaagagg gggccaaccc cgaacgggtc cgcaccgagc
     2161 tcgcccagga gggaatccag cccgaggaat ggggcgggga caccgtcttc gccgaggtct
     2221 cggccaagac ccacgagggg ctcgacaacc tgctcgacat gatcctcctg gtcaccgagc
     2281 tggaggaact cggcgccaac cccgacgcgc ccgcttcggg caccgtgatc gagtcccagc
     2341 tcgaccccgg ccgcggcccg gtcgtcaccg tgctggtcca gcgcggcacc ctgcgcgtcg
     2401 gcgactcgct ggtcgccggt ccccagtggg gccgggtgcg cgcgatgcac gacttcaccg
     2461 gcgcccgcgt cgaggaggcg gagcccggga tgccggtcga ggtgctcggc ttcgacgggg
     2521 tctccgatgc cggcgaattc gtccaggcgg tcgagaacga ccgccgcgcc cgccagcttg
     2581 cccaggagcg cgcagaccgg ctcaaacgtg aggcgctggc ccgtcgcaac gcccgcaagg
     2641 tcagcctcga cgaggttttt gccagggctc agcagggcga gatcaaagaa ctcaacatcg
     2701 tcctcaaggg cgacgtcgcc ggctccctgg aggcgctcca ggacgagatc gccaaggtcc
     2761 cgcaggaccg cgtcgcgatc aacatcatcc actcgcagac cggtggcatc aacgagtccg
     2821 acgtgatgtt ggcctcggct tccgacgcga tcatcatcgg tttcaacgtg cgcccgctgc
     2881 tcgatgccaa acgcgccgcc gagcgcgagg gcgtcgacgt ccgcacctac tcggtcatct
     2941 acaaaatcac cgaggacctg cgcaacgcga tggagggctt gctggaggcg ctcgaggtcg
     3001 aggagacggt cggcgaggcc gaaatcaaac agaccttcaa agcctcgaag gtcggccgca
     3061 tcgccggctg catcgtcacc cagggcaagg ccgtgcgcag cgccagtgtc cgcctgatcc
     3121 gcgagggcac ggtgatctgg accggcgagc tcgcctcctt gcgccgcttc aaagacaacg
     3181 tgcaggaagt cgaggaaggc caggagtgcg gaatcgtgct tgacgggtac gccgacgtca
     3241 aggaggacga cgtgatcgag ttcttccaga ccaaacaggt cgagcagacc ctggagtgat
     3301 ggtcggagcg taagatggcc ggcgctcgca tgcggcgcat caacgaggtg cttcgcgagg
     3361 tcgtgggcgc cacgattgca ggcgacctca gcgacccccg catcggcttc gtgaccgtga
     3421 ccgccgtaga gaccagcccc gatcttcgct ccgccaaggt gtacgtcagc gtgctcggcg
     3481 atgaagagga gcgcgaggcg acgctcagcg ccctacgctc cagccacggc gtgatccagt
     3541 cgcggatcgc cgccgagacc aggatgaaga ggacccccac cttgaccttc cactacgacg
     3601 acacgatcga gaaaggcgtg cggatctcgc acctgctcga ggagaccgcc gactccggcg
     3661 agggggacga gcggtgagcg aggcaaccgg cttggccacc tcctcggaca tcgagcgcgt
     3721 cggtgcggag ctgcgcgccc gccagcgctt cctgctgacc gcccacgagg gcccggacgg
     3781 cgacgcgctc ggctcgctgc tcggcatgca ccacctgctc acgcagctcg gcaaggactc
     3841 ggtgatgttt ctcgccgcga aggagttccc gctgccgatc gagtaccgct tcctgccgct
     3901 ggaggaggtc ttccacgagg cgccggccga catggccgac cgcaccgtcg tcttcctcga
     3961 ctgcggcaac atcgaccgca tgccggtcga cttcctcacc gacggcgaca acttcacgct
     4021 caacatcgac caccaccacg acaacacccg cttcggcgac gtcaacctcg tcgacgtgga
     4081 ggcgtcgtgc acggccgaga tcgtctacga cctggcgaag ctgctcggcg ccgagatcac
     4141 tcagccgatg gccgcggcgc tctacgtcgg cctgatcacc gacaccggca agttcatgta
     4201 cgagaacacc aacgcgcaca cgcaccgggt ggccgctgag ctgatcgacg cgggggttga
     4261 cgtcgacgac acctaccgcc gcctctacga gcacgtgccg ctggagaagc tgcgcctggt
     4321 cgcccgggcg ctcgggggga ttcagcgcca ctgccgcgac cggctcgcga tcagctacat
     4381 caccgccgcc gactacgagg cgaccggagc cggcgaggag atgaccgagg gcgtgatcga
     4441 ccacctgcgc tcgatcgagg ggatcaaggt tgccgccgtg gttcgcgacc tcggcaaccg
     4501 cggccgcgcc gcccgcaagg tcagcctccg ctccagtgag ggcgacgtgg acgtttcggc
     4561 gatcgcccgg ctgaacgggg gagggggaca caaacgcgcc gcgggtttct ccaccgacct
     4621 cgagctagac gacctcgtga cgttcctctg tgaacaggta gtcgcgcagc tcggcgagtg
     4681 agcgtggcga tgaccgagac cggtggggca gtgctgctct atgacaagcc ggccggggtc
     4741 acctcgcacg acatcgttgc ccgggtgcgc cgcgagcgtg gcggtaaaac cggacacgcg
     4801 ggcaccctcg accccttcgc gaccggcctg ctgctgatcc tgcgcggtcg ggcgacgcgc
     4861 ctgcagcgct tcctggtcgg cctgccgaag acctacctgg cgacggcgcg gctgggctgg
     4921 cgctcgagca ccggcgaccc cgatggcgag ctgaccgaga cgggggccat ccccgactcg
     4981 ctggagctgc cgaccggaac cgtgcgccag cgggtgccga tgacctcggc ggtccgggta
     5041 ggcggcgagc gcctctacaa gaaggcccac cgcggcgaag tcgtcgagac gcccgagcgc
     5101 gacgttgagg tccaccgcgc cgagctgctc tcggccgccg acgggcgcgc ccactacgag
     5161 atcgagtgct cggccggaac ctacgtccgc accctgatcg agaccctcga cgacgcctac
     5221 tgcgaggagc tgcggcggac cgcgatcggc cccttccggg tcgaggacgc gggcgaggag
     5281 ctgccggtcg agcgggtcgc cgagctggtt cccgcggttg cggaggcgct gcgatgagca
     5341 tcgaggtgac gatgctggcc gacgtcaagc ggcgcccgcg ccacgttgcg atcgggacct
     5401 tcgacggcgt ccacctcggc caccaggcgg tgatcgatca cgccgacacc gtgctcacct
     5461 tcgacccaca cccgatgtcc gtattgcgcc cggaggcggc gccgccgctg atctcgacct
     5521 tctcggtgcg ccgcgacgtg cttgacgggc tcggcgtcgc agagctggtc gtaatcccct
     5581 tcgacaagga gttctcgcag ctcggtccga acgaattcat cgaggacgtg ctgatcgagc
     5641 ggctcggtgc ggtgaaagtg tctgtcggcg agaacttcca cttcggcgcc aaagccgagg
     5701 gcagcccgca gctgctcgcc gaccgcaccg agttcgagac caggatcgtg ccgctgatcg
     5761 aaatcgacgg cgacaccgtc tcctccagcc gcatccgcga gcagatcgcg aacggcgaag
     5821 tcgccgccgc gcgccgctgc ctcggcgccc ccttcatggt cgagggaacc gtcgtcaaag
     5881 gtgaccagcg tgggcgggag ctcggcttcc cgaccgccaa catcgttcct gacgagcgtc
     5941 tcgtcacccc cggccacggc gtttacgcag ccttcgccaa cggcgccccc gccgcggtca
     6001 acgtcggcgt gcggccgacc ttcgagtccg agctcggggt gctgatcgag acctacctgc
     6061 tcgaccgcga cgaagacctg tacggcaaga ccctgcgcgt cgccttcgtc gaaaagctgc
     6121 gtgacgagga gcgcttcgag caggtcgacg agctgatcga gcagatgaaa cgcgacgtcg
     6181 aggacgcgcg cgaggtctgc gccagcttca cccacagcgg cgagccttga tccggtgcgg
     6241 ctcgcaactt cgcgagcgcg gcaggtttca agcccccgaa tgaggtgtct gggtcatatg
     6301 ccgctgcgcg cgggcgcgct cgcggcgctg agcacgctcc tcgcagcgct cgcgctcgcc
     6361 ggcaccggca gcgctgcccc aaatcctgac atcgctgcct tcaggatcgg ccacggagcc
     6421 tgggcaacgg gcttcacgct gggcccggac ggcaggttct ggtttgtcgg gaacaccggc
     6481 aacaccgaca aggcgcaccc cgaagggatg gtcgggtggt tcacgacgga cggcgcgtcc
     6541 gacgaagcac ggttgccgag tcactcggtc ccgggcggcg gcaagatcgc cgacggcccc
     6601 gacggccgcc tctggttcac cgatccggtg acgaaccagg tcggcgtcgt caacgcggtg
     6661 ggtctgttcg agcagtaccc gctgccgacc cctggtgcct ctccgggcgc gatcgtctcc
     6721 ggcccaggag acgcgatgtg gttcgtcgag gaaaacgccg acaaggttgg ccgggtcgag
     6781 atggacgggg acatcggcga gacgtcactg cccgagggcg cgcagccgac ggggatcgtc
     6841 gaaggctccg acggggcgct ctggatcgtc gcgaagggcc tcggcaagat cttgcggatg
     6901 aactccaatg gagaggtcag tgacgagtac gcgctgcctg accccgacag ccgcccgcac
     6961 gcgatcgtcc acggccccgg aggcgacctt tggtttagcg aggaagacgg cccccggatc
     7021 ggccgcatca acgcggctgg cgcaatcacg gagtacccga tccccggcga gaacgggacc
     7081 cgcgagctgg cgttgggagg tgacgggaac ctctggttca ctgccggttc ggcgatcggc
     7141 tcgatctcgg ccagtggcga gaccggtgaa ccagagtgcg tctcgaacgg ctgtgggtac
     7201 ccgatcaacg ccctggcaaa aggaccggaa ggcgggatct ggttcgcaac cgacatcttg
     7261 gagtccggag aagctggagg gggcacgccg cagctcgcct atctcgcggg cggtttggtt
     7321 ggcaggttcc tcgcaccgga gctgagcgtc cggctcggca ggcgggcgac cagggtcgac
     7381 gacgggttga ccacgatcgc gatcagctgc cacgggggca ccgccggcga agcctgtcgg
     7441 ggctggctgc gcctgaccac gaagctgcgc gggcgcaagg tgctgctcga ccagcaccgc
     7501 taccggctcg agccggcgac ctctcggcgg ctgccgctcc gcctcggcgc ccgcggcaag
     7561 cgcaccgtgg cccgcgccaa gcggatcaag gtccagatct cggcgagcct gatcgaaggg
     7621 tccggcgcgc gacgcggctt cgtcctgcag gcacgccgcc ggcgctgatc gaccctgctc
     7681 cgagcgtcgc cgtctgctac cttccagcgg ccgtgacccg aggggtgcac ggcttttttg
     7741 agactgacat gagcctgacc aaagaaaaaa agagcgagct gatcggcaaa tacggtcgcg
     7801 ccgagggcga caccggttcc gccgaggtcc agatcgccct gctcaccgag cgcatcaacg
     7861 acctcaccgg tcacctgcgc gagcacagca aggaccacca ctcacgccgt ggcctgctga
     7921 tgctggtcgg caagcgccgc cgcatgctcc gctacctcga gcgcaacgac ctcgagcgct
     7981 accgcgccct ggtcgccgag ctcggcctgc gtcgttgatc gcagtcggcg ataaggcgcc
     8041 cgacttcacc ctccgcgacc aggacggtga agacgtctcg ctcgccaact tcaagggccg
     8101 caaggtcctg ctggtcttct acccgatgga cttcagcccg gtctgctcgg accagctctc
     8161 gatcta
//
LOCUS       DASRIG010000346         9909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3837284, whole genome shotgun sequence.
ACCESSION   DASRIG010000346 DASRIG010000000
VERSION     DASRIG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837284"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFB52_09355"
     CDS             <1..776
                     /locus_tag="VFB52_09355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZO06582.1"
                     /translation="LIGALEPLTYTGTMQSSNAGDHPFTRLARTAKIQETVFLGTREE
                     ADKALAAVHRLHERIKGELAEDAGAHKAGAAYSAFDPELMLWTLAVIADSGRVMYETM
                     ARPLSEAEREALWQDYVRFGELFGLPASEVPGSYREFQAWFDGRLASSDMHATPHALE
                     MAPMVAFEQPVPPSARANIATQNLVIKGTLPPRVREIFGIEWTRGHEAAFRAVTSAHR
                     RARPAFPRRMRRGRNDYFFDVVTKAEKRRGGSPTPQVRA"
     gene            complement(773..1843)
                     /locus_tag="VFB52_09360"
     CDS             complement(773..1843)
                     /locus_tag="VFB52_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO06583.1"
                     /translation="MDEQWIRFTEVDGRRVAWAVVGEGPPLVMGGWWMSHLEMNWRDP
                     PFRAFVEAIARHRTVVRYDSPGTGLSDRSQPPSTSLAHHVAVLAGVVDALGAESVDLF
                     AASSGGPVTAAYAAANPQRVRRLVAYGTYASGPAIADEDARASIVGIVRRHWGVGSRV
                     LTDVFMPEADPTQRKAFVEFQRQSASAELAARALECVYSFDVVDSLGQLQAPVRVLHR
                     RGDRAIPYRLGQDLATRIPGATFVALEGSDHFPWLGRPGEALRAVLEGLGVDPAEIEV
                     DPEVEQTPVAGNGEVAETDLSERELEVLRLVALGLSDREIAERLVLSPHTVHRHVANI
                     RTKLRLPSRAAAAAQAARLGLI"
     gene            1970..2611
                     /locus_tag="VFB52_09365"
     CDS             1970..2611
                     /locus_tag="VFB52_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO06584.1"
                     /translation="MYATESIEVAESKFGAAIYEPFLWLGEKLGMARRRARLLAGARG
                     RVLEIGAGTGLNLRHYPAAIEELVLVEPAETMAAKIDTSGFPGDSPVSVVLSPAERLP
                     FADDSFDTVVSTMVLCTVSDPEQALAEISRVLRPGGKLLFCEHVASDGRVLGGLQRRL
                     AGPWAAFAEGCRCDRRTVETIDREMRLGPIERDNWRGMAPLVRPLVIGTAEPR"
     gene            2719..3372
                     /locus_tag="VFB52_09370"
     CDS             2719..3372
                     /locus_tag="VFB52_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZO06585.1"
                     /translation="MAASAALLLLPATAPAAGSVSVTGKATLAVPNDTAAVGLGSKAV
                     RPTKAAALRVVAKRVRAIVAAAQAIPGVGDGDVRTGSVRLSQTSRGKRVLWGASQGIT
                     VTLHQPERAGELIDAGVRAGASTVNGPRFYLGDASAAYNTALVAAFDQARAKAEVLAA
                     RAGATLGEVTAISEGGGVEPVQAGSPQRGESKADGAPPTKPSTTTVTATVSVVFALQ"
     gene            3387..4355
                     /locus_tag="VFB52_09375"
     CDS             3387..4355
                     /locus_tag="VFB52_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO06586.1"
                     /translation="MLAAYAAKIDREDPLAGLEIGEVDAPTVPDGWVEVTVKAAALNH
                     HDVWSLRGQGLREEQLPMILGCDAAGLDPDGNEVLVHAVISSPEWRGDETFDPKRTLL
                     SELHPGTLAERVAVPAGNVVPKPAGLSFAEAACLPTAYLTAYRMLFTRSNLKPGNTVL
                     IQGAGGGVASAAIQLARAGGFRVWATSRDERKRAYAEELGAHATFETGERLPAKVDAV
                     METVGEATWEHSLRSLRPGGRIVVSGATSGFNPPADLPRVFFLQLEVVGSTMGTREEL
                     GRLVELCDAAGVRPQIDRTLALADVDQGIAALVAGDVLGKIVVEVG"
     gene            4366..4758
                     /locus_tag="VFB52_09380"
     CDS             4366..4758
                     /locus_tag="VFB52_09380"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HZO06587.1"
                     /translation="MAWVEVFLVLVVSHLVGDFLLQTDWQAKYKYGGLGPDPVRRRAL
                     GTHIVTYMLCFVPALVWIAAEGGLARAVLAAIVVAVPHWVQDDGRLLDLYMAAVKGLS
                     PTSPGLRVAVDQSFHVVALFLTALIISS"
     gene            complement(4755..6533)
                     /locus_tag="VFB52_09385"
     CDS             complement(4755..6533)
                     /locus_tag="VFB52_09385"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZO06588.1"
                     /translation="MLIVILCAAALAVTAYATSLMRSLELNSVDARFSVRGTQERPDD
                     IVVVGVDDVTFNDLGVQWPFPRRLHARVIDRLREAGAKVVAYDVQFTEPTTFRDDNAL
                     IEAIARKGGVVLSTTEVDRRGNTRVLGGEEVLAEVEASAANGNYEPDPGGVVRKMHYQ
                     LDGLVTFAVATAEAATGETVEPTEFESDGEAWIDYRGPPDTFRNVPFVRVLRGQVPDA
                     VFRGKTVIIGATHPTLQDIHATPTTGDEYMSGPEIQANAVWTAEHGFPLGSSSDIVDL
                     LLILLMAVTPAAITLRLKPPLALVSALALGILYAVLTQVAFNGGVILPVVYPLLALVL
                     SALGALSVNYVLTAFERQRVRDTFSRFVPEAVVADVLARGDGDLRFGGVRRECTVLFS
                     DLRGFTTFSEALPPDRVIEVLNHYLEEMSDAIMDHGGTLVAYMGDGIMAVFGAPTDQT
                     NHADRAVEAAREMLNVRLPRFNEWMVGNGFEQGFAMGIGLNSGTVMSGQVGSKRRIEY
                     TAIGDTTNTAARLEGMTKGSGHQLFIAESTRLALEADPGDLELIGERDVRGRAHKITV
                     WTLEGPKNPLADPPLADGPPADDAPL"
     gene            complement(6572..8383)
                     /locus_tag="VFB52_09390"
     CDS             complement(6572..8383)
                     /locus_tag="VFB52_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZO06589.1"
                     /translation="MNRLVVTLAVIAAALVVPASASALNFEVNTEDDLNLGTEACTPT
                     FNCSFHDAIDRANETAEADEITFGELFAGKFEPEAPLPPIIWPVEIDATTAADYSDAG
                     HPVLLLSGEAISDPEKRGLEFGEDAGGSSVEGLAVGGFRLGIFTYAEAGVDICANYVG
                     VETDGETALPNVYAGISTGATGAPTQGARIGGAGCAGNLISGNTEFGILEAGEDTVIK
                     GNSIGVDAEGEPLPNGDSLLVSAGIEVNAIAKDPVIGGIMPGEGNLIAYNEGQGIRVR
                     DSASTAKIRGNSIHSNLLSELQIDTGLITAVPELKFVSSDGVKAKAQGAIVDAAPNEQ
                     YAVDVFVNAACPSGEPAGETYLGEVAVETDATGFASFSTESLAPAPPGSELFTTTATP
                     AVTGSTSTFSACFDETPAPPPPAPPVDPPTQPIVQIQPVNGESVVVAPAGGKVFVQRP
                     GEKKPTELKEGQTIPVGSIVDATNGKVTLTSVNKAGETQTAVFYGGKFLVAQQEGSGL
                     VVLKLRGSLKGCPKAGDSSATASGKKGRRLWGSGKGKFRTEGNYGSATVRGTVWLTED
                     RCTGTFFKVRKGVVTVRDFPAKSTVSLGKGKSYFAKP"
     gene            complement(8380..>9909)
                     /locus_tag="VFB52_09395"
     CDS             complement(8380..>9909)
                     /locus_tag="VFB52_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06590.1"
                     /translation="IDGTTAENYEGTPVIEIDASGAAAEQGLRIDAPKTEIRGLAITG
                     AVGPGLFFSSEPEGVSCGNYVGVDVTGAAAANGSGIQISSDAEKVRIGGLACGNLISG
                     NEGNGINAEGFDASISGNLIGTDASGEAPLPNTGNGIRATFFADSTAINAVGGVPNTI
                     AFNGGAGVLVESAEAAVVAIYANSIFDNDDLGIKILLEDGLTPPTIEAVKYTAPVSIS
                     GTVTGEPNTTYFIDFYANEACDPSGAGEGQTYLSDWDVTTNASGIGKFTAEEGGEGEG
                     EEGLDPIPAGQKVITATSSTQFSEAGEGTSEFSKCFTGKEVTPPPPPPPPPPAQTLAL
                     EPVNGESLAVEPVSGTVFIKLPGKGKQVKLTEGMLIPVGSVVDATKGKVTLTSVNKAG
                     EAQTAVFYGGKFLVAQRDGSGLVTLQLRGALKGCTKAGSSMATASGKKGRRLWGSGKG
                     KFRTEGNYGSATVRGTVWLTEDRCTGTFFKVKKGVVTIRDFSANETFPLGKGKTYFAQ
                     P"
BASE COUNT         1379 a         3456 c         3489 g         1585 t
ORIGIN      
        1 tgctgatcgg cgccctcgaa cccctcacct acaccggcac gatgcagagc tccaacgccg
       61 gcgaccatcc cttcacccgc ctcgcccgca cggcgaagat ccaggagacg gtcttcctcg
      121 gcacccgcga ggaggccgac aaagcgctgg ccgcggtgca ccgcctgcac gagcggatca
      181 agggcgagct ggcggaggac gccggggcgc acaaagccgg cgccgcctac tcggccttcg
      241 acccggagct gatgctgtgg acgctggcgg tgatcgccga ctcgggccgg gtgatgtacg
      301 agacgatggc gcggccgctg agcgaagccg agcgcgaggc gctctggcag gactacgtcc
      361 gcttcggcga gctgttcggg ctgccggcca gtgaggtccc cggcagctac cgcgagttcc
      421 aggcctggtt cgacggccgg ctcgcctcct ccgacatgca cgcgaccccg cacgcgctgg
      481 agatggcgcc gatggtcgcc ttcgagcagc cggtgccgcc gagcgcccgc gccaacatcg
      541 cgacccagaa cctggtcatc aagggcaccc tgccgccgcg ggtgcgggag atcttcggga
      601 tcgagtggac ccgcggccac gaggccgcct tccgcgccgt gaccagcgcc caccggcgcg
      661 cccggccggc cttcccgcgg cgcatgcgcc gcggccgcaa cgactacttc ttcgacgtcg
      721 tcaccaaagc cgagaagcgc cgcggcggct caccgacccc gcaggtccgc gcctagatca
      781 agccgaggcg ggcggcctgg gcggcggccg cggcgcgcga gggcaggcgc agcttggtgc
      841 ggatgttggc gacgtggcgg tgcacggtgt gcgggctgag caccaggcgc tcggcgatct
      901 cgcggtcgct gagcccgagc gcgaccaggc gcaggacctc gagctcgcgc tcggagaggt
      961 cggtttcggc gacctctccg tttcccgcca ccggcgtctg ctcgacctcg ggatcgacct
     1021 cgatctcggc ggggtcgacg ccgaggccct cgagcacagc gcgcagggct tcgcctgggc
     1081 gaccgagcca ggggaagtgg tcggagccct cgagcgcgac gaaggtggcg ccggggatcc
     1141 gggtcgcgag gtcctggccg agtcggtaag ggatcgcccg gtcaccgcgg cggtgcagca
     1201 cccggaccgg tgcctgcagt tgcccgaggc tgtcgacgac gtcgaaggag tagacgcatt
     1261 cgagcgcccg cgccgccagc tcggcggaag ccgactgacg ctggaactcg acgaaggctt
     1321 tgcgctgggt agggtcggcc tccggcatga agacgtcggt gaggacgcgc gaaccgaccc
     1381 cccagtggcg gcggactatc ccgacgatcg aggcacgggc gtcctcgtcg gcgatcgccg
     1441 gtccgctcgc gtaggtcccg tacgcgacca gccgccgcac ccgctgcggg ttggcggccg
     1501 cgtaggcggc ggtgaccggt ccgcccgaag aggcggcgaa gaggtcgacc gactcggccc
     1561 cgagcgcatc gacaaccccg gccagcaccg cgacatggtg ggcgaggctg gtcgacggcg
     1621 gctgcgagcg atcggagagg ccggtgcccg gcgagtcgta gcggaccacc gtgcggtggc
     1681 gggcgatcgc ttccacgaag gcccggaacg gcggatcgcg ccagttcatc tccaggtggc
     1741 tcatccacca gccgcccatc accagcggcg gtccctcccc caccacggcc caggcgacgc
     1801 gacggccgtc gacctccgtg aaccggatcc actgctcgtc catcgccgca aggctagcgc
     1861 cggatggccc gtcccggcca tcgcaagttg gccgttccgg gcgaagtcac ggagccgccc
     1921 cggtggtcga atgcggatac caccttccca ttcgacgaaa ggaccaagga tgtatgcaac
     1981 ggagtcgatc gaggtcgccg aaagcaagtt cggcgcggcg atctacgagc cctttctctg
     2041 gctcggcgag aaactcggga tggccaggcg ccgcgcccgc ctgctcgccg gcgcccgggg
     2101 ccgcgtgctc gagatcggcg ccggcaccgg cctcaacctg cgccactacc ccgccgcgat
     2161 cgaggagctg gtcctggtcg agccagccga gacgatggcg gcgaagatcg acacctcggg
     2221 cttcccgggc gactcgccgg tcagcgttgt cctctccccc gccgagcgcc tcccgttcgc
     2281 cgatgactcc ttcgatacgg tcgtctcgac catggttctc tgcacggtca gcgaccccga
     2341 gcaggcgctc gccgagatca gccgcgtgct ccgccccggc ggcaagctgc tcttctgcga
     2401 gcacgtcgca tccgacggcc gcgtgctcgg cgggctgcag cgccgcctcg ccggcccctg
     2461 ggcggccttc gccgagggct gccgctgcga ccggcgcacc gtcgagacga tcgatcggga
     2521 gatgcgcctg ggcccaatcg agcgcgacaa ctggcgcggc atggccccac tcgtgcgccc
     2581 gctggtaatc gggacggccg agccgcgctg aggtcgccgc gccgtatgca ctttgtcgcc
     2641 ctctgggcta caaagcgagt acggcgcgcc gatcggggta gcgttgcggc aatgggttct
     2701 cggcgatggc ttgttttggt ggcggcgtcg gcggcgctgc tgctcctgcc ggcgacggcg
     2761 cccgccgccg gctcggtctc ggtgaccggc aaggcgacgc tcgccgttcc caacgacacc
     2821 gccgcagtcg ggctcggaag caaagccgtg cggccgacca aggccgccgc tttgcgcgtg
     2881 gtcgccaagc gggtgcgggc gatagtcgcc gccgcccagg cgatccccgg ggtcggcgac
     2941 ggcgacgttc gcaccggctc ggtcaggctg agccagacct cccgcgggaa acgcgtgctg
     3001 tggggcgcca gccagggcat caccgtcacg ctgcatcagc ctgaacgggc cggcgagctg
     3061 atcgacgccg gagtacgggc cggcgccagc accgtgaacg ggccgcgctt ctacctcggc
     3121 gatgccagcg ccgcctacaa caccgcgctc gtcgccgcct tcgaccaggc gcgtgccaag
     3181 gccgaggtgc tggccgcgcg agcgggcgcg acgctcggcg aagtgaccgc gatcagcgag
     3241 ggcggcgggg tcgaaccggt gcaggccggc agcccgcagc gaggcgagtc gaaggccgac
     3301 ggcgccccgc cgaccaagcc gagcacgacc accgtgaccg ccaccgtcag cgtcgtcttc
     3361 gcgctgcagt agtaggttcg agccccgtgc tcgccgccta cgccgccaag atcgatcgcg
     3421 aggacccgct cgcgggactc gagatcggcg aggtagacgc gcccaccgtc cccgacggct
     3481 gggtcgaggt cacggtcaaa gcggcggcgc tgaaccacca cgacgtctgg tcgctgcgcg
     3541 gccagggcct gcgcgaggag cagctgccga tgatcctcgg ctgcgacgcc gccggactcg
     3601 accccgacgg caacgaggtc ctcgtgcacg ccgtgatctc cagccccgag tggcgcggcg
     3661 acgagacctt cgacccgaag cgcacgctgc tctccgagct gcacccgggc acgctcgccg
     3721 agcgggtcgc ggtgccggcc ggcaacgtgg tgccgaagcc ggccgggctg agcttcgccg
     3781 aggcggcctg cctgccgacc gcctacctga ccgcctaccg gatgctcttc acccgctcca
     3841 acctcaagcc cggcaacacg gtgctgatcc agggtgccgg cggcggcgtc gcctcggccg
     3901 cgatccagct cgcccgcgcc ggcggcttcc gcgtctgggc aaccagccgc gacgagcgca
     3961 agcgcgccta cgcggaggag ctcggcgccc acgccacctt cgagaccggc gagcgcctgc
     4021 cggcgaaggt cgacgcggtg atggagacgg tcggcgaagc gacctgggag cactcgctgc
     4081 gctcgctccg ccccggcggg cggatcgtcg tctccggcgc cacctccggc ttcaacccgc
     4141 ccgccgacct gccgcgcgtc ttcttcctgc agctcgaagt ggtcggctcg acgatgggca
     4201 cccgcgagga gctcggccgg ctggtcgagc tctgcgacgc cgccggcgtg cgcccgcaga
     4261 tcgaccgcac gctggcgctg gccgacgtcg accaggggat cgcggcgctg gtcgccggcg
     4321 acgtgctcgg caagatcgtg gtcgaggtcg ggtaggagcc ccgggatggc ctgggtcgaa
     4381 gtcttcctcg tgctggtcgt cagccatctg gtcggcgact tcctcctgca gaccgactgg
     4441 caggcgaaat acaagtacgg cgggctcggc cccgacccgg tccgccgccg cgcgctcggc
     4501 acccacatcg tcacctacat gctctgcttc gtcccggcgc tggtctggat cgccgccgaa
     4561 ggcgggctgg cgcgggcggt gctggcggcg atcgtcgtcg ccgtccccca ctgggtccag
     4621 gacgacggcc gcctgctcga cctctacatg gccgcggtca aggggctgtc cccaacctca
     4681 cccggtctgc gggtcgcggt cgaccagtcc ttccacgtcg tcgcgctctt cctcaccgcg
     4741 ctgatcatca gcagctagag cggcgcgtcg tcggcgggcg gaccgtcggc caagggcgga
     4801 tcggccagcg ggtttttcgg tccttccagg gtccagaccg tgatcttgtg ggcgcggccg
     4861 cggacgtcgc gctcgccgat cagttcgagg tcgccggggt cggcctccag cgccagccgc
     4921 gtcgactcgg cgatgaacag ctggtggccg gagcctttgg tcatcccctc cagccgcgcc
     4981 gcggtgttgg tggtgtcgcc gatcgccgtg tactcgatcc gccgtttcga tccgacctgg
     5041 cccgacatca cggtgccgct gttcagcccg atccccatcg cgaacccctg ctcgaacccg
     5101 ttgccgacca tccactcgtt gaagcgcggc agccgcacgt tgagcatctc gcgcgccgcc
     5161 tcgacggcgc ggtcggcatg gttggtctgg tcggtcggcg ccccgaacac ggccatgatc
     5221 ccgtcgccca tgtaagcgac cagggtgccg ccgtggtcca tgatcgcgtc gctcatctcc
     5281 tccaggtagt ggttgaggac ctcgatcacg cggtcgggag gcagcgcctc ggagaaggtg
     5341 gtgaacccgc gcaggtcgct gaacagcacc gtgcactcgc gccgcacgcc gccgaagcgc
     5401 agatcgccgt cgccgcgcgc gagcacgtcg gcgacgaccg cctcggggac gaagcgggag
     5461 aaggtgtcgc gcacgcgctg gcgctcgaac gcagtcagca cgtagttgac cgagagagcg
     5521 ccgagcgccg agaggaccag cgccagcaac ggatagacga ccggcaggat cacgccgccg
     5581 ttgaaggcga cctgggtcag caccgcgtag aggatcccca gggcgagggc cgagaccagc
     5641 gccagcgggg gtttcagccg cagcgtgatc gccgccggcg tcaccgccat cagcaggatc
     5701 agcagcaggt cgacgatgtc gctggaagag ccgagcggga agccgtgctc ggcggtccag
     5761 accgcgttgg cctggatttc gggacccgac atgtattcgt cgccggtggt cggggtcgcg
     5821 tggatgtcct gcagggtcgg gtgggtggcg ccgatgatca cggtcttgcc gcggaagacc
     5881 gcgtcgggca cctggccgcg cagaacgcgc acgaagggga cgttgcggaa ggtgtccggt
     5941 ggaccgcgat agtcgatcca ggcctcaccg tcggattcga actcggtcgg ttcgaccgtc
     6001 tcgccggtcg ccgcttccgc cgtggcgacc gcgaaggtca cgaggccatc cagctggtag
     6061 tgcatcttgc ggaccacccc gccggggtcg ggctcgtagt tgccgttggc ggcgctcgct
     6121 tcgacttctg caaggacttc ctccccgcca agcactcgcg tgttcccgcg ccggtccact
     6181 tcggtggtcg agagcacgac gccgcccttg cgggcgatcg cttcgatcaa cgcgttgtcg
     6241 tctcgaaacg tggtcggctc ggtgaactga acgtcgtagg cgacgacttt ggcgccggcc
     6301 tcgcgcagcc gatcgatcac ccgcgcgtgc agccgccgcg ggaacggcca ctgcacgccg
     6361 aggtcgttga aagtgacgtc gtcgacgccg accacgacga tgtcgtcggg ccgctcctgc
     6421 gtgccgcgca ccgagaaccg ggcgtcgacc gagttcagct ccagcgagcg catcaggctg
     6481 gtcgcgtagg cggtcaccgc cagcgccgcg gcgcagagga tcacgatcaa catcgcgact
     6541 tcccggcgcc gtttgggcga gtgccgcgcg ctcacggctt ggcgaagtag ctcttgccct
     6601 taccgaggga gaccgtgctt ttcgccggga agtcgcgcac ggtcacgacc cctttgcgga
     6661 ccttgaagaa ggtcccggtg cagcggtctt cggtcagcca gacggtgccg cgcacggtcg
     6721 ccgagccgta gttgccttcg gtgcggaact tgcccttgcc gctgccccag aggcggcggc
     6781 ccttcttgcc ggaggcggtc gcgctcgagt cgccggcctt cgggcagcct ttcagcgagc
     6841 cgcgcagctt caggacgacg aggccgctgc cctcctgctg ggcgacgagg aacttgccgc
     6901 cgtagaagac cgcggtctgc gtttcgcccg ccttgttcac cgaggtgagc gtgactttgc
     6961 cgttggtcgc gtccacgatc gagccgaccg ggatcgtctg gccttccttc agttcggtcg
     7021 gctttttctc cccgggccgc tgcacgaaca ccttgccgcc ggcgggcgcg accacgaccg
     7081 actcgccgtt gaccggctgg atctggacga tcggctgggt cggcggatcg actggagggg
     7141 ctggcggcgg cggggccggc gtttcgtcga agcaggcgga gaaggtcgag gtcgaaccgg
     7201 tcaccgcggg ggtcgcggtg gtggtgaaga gctcgctgcc gggcggcgcc ggcgccaggc
     7261 tttcggtgga gaagctggca aaccccgtcg cgtcggtttc caccgcaacc tcgccgaggt
     7321 acgtttcgcc cgccggctcg ccgctcggac aggcggcgtt gacgaagacg tcgaccgcgt
     7381 actgttcgtt cggcgcggcg tcgacgatcg ccccctgggc tttggctttg acgccgtcgg
     7441 aggagacgaa tttcagctcc ggcaccgcgg ttatgagccc ggtgtcgatc tgcagctcgc
     7501 tcagcaggtt cgagtggatc gagttgccgc gaatcttggc cgtgctggcg ctgtcgcgga
     7561 ccctgatccc ctggccttcg ttgtaggcga tcaagttgcc ttcgcccggc atgatgccgc
     7621 cgatcaccgg gtctttggcg atcgcgttga cttcgatccc ggcgctgacc aggagcgagt
     7681 cgccgttggg cagcggttcg ccttcggcgt cgacgccgat cgagttgcct ttgatcacgg
     7741 tgtcctcgcc ggcctcgagg atcccgaact cggtgttgcc ggagatcagg ttcccggcgc
     7801 agccggctcc gccgatccgg gcgccttgcg tgggcgcccc cgtggccccg gtcgagatcc
     7861 cggcgtagac gttgggcagc gcggtttcgc cgtcggtttc gaccccgacg tagttggcgc
     7921 agatgtcgac cccggcttcg gcgtaggtga agatgccgag ccggaagccg cccacggcga
     7981 gcccttcgac gctgctgccg ccggcgtctt cgccgaactc cagcccgcgc ttttccggat
     8041 cggagatcgc ttcgccggag agcagcagca ccggatgccc ggcgtcggag tagtcggccg
     8101 cggtggtcgc gtcgatctcg accggccaga tgatcggcgg cagcggcgcc tccggttcga
     8161 atttgccggc gaacagctcg ccgaaggtga tttcgtcggc ttcagcggtt tcgttggcgc
     8221 ggtcgatcgc gtcgtgaaag ctgcagttga aggtcggggt gcacgcttcg gtcccgaggt
     8281 tgaggtcgtc ttcggtgttg acttcgaagt tgagcgccga cgcgctcgcc ggcacgacca
     8341 gggccgccgc gatcacggcg agcgtgacta cgagccggtt cacggctgcg cgaagtaggt
     8401 cttgcccttg cccagcggga aggtttcgtt ggcgctgaag tcgcggatcg tgacgacgcc
     8461 ttttttcacc ttgaagaagg tcccggtgca gcggtcttcg gtcagccaga cggtgccgcg
     8521 cacggtcgcc gagccgtaat tgccttcggt gcggaacttg cccttgccgc tgccccagag
     8581 gcgccgaccc ttcttgcccg aggcggtggc catcgagctg cccgctttcg tacagccttt
     8641 cagcgcgccg cgcagttgca gcgtcaccag gccgctcccg tcacgttggg cgacgaggaa
     8701 cttgcccccg tagaagaccg cggtctgcgc ctcacccgct ttgttcaccg aagtcagggt
     8761 gactttgcct ttggtcgcgt cgacaaccga gccgaccggg atcagcatgc cctcggtcag
     8821 tttcacctgt ttgcctttgc ccggcagctt gatgaagacc gtgccgctca ccggttcgac
     8881 cgcgaggctc tcgccgttga ccggttcgag cgcgagcgtc tgcgccggtg gcgggggcgg
     8941 cggtggaggc ggcggcgtca cttctttgcc ggtgaagcat ttcgagaact ccgaggtgcc
     9001 ttcgccggcc tcggagaact gggtcgagga ggtcgccgtg atcactttct ggccggcggg
     9061 gatcgggtcg agcccttctt caccctcgcc ttccccgcct tcctcagcgg tgaatttgcc
     9121 gatgccgctg gcgttggtag tgacgtccca gtcggagaga taggtctgcc cctcgcccgc
     9181 accactcggg tcgcaggctt cgttggcgta gaagtcgatg aagtaggtcg tgttcggttc
     9241 gccggtgacc gtgccgctga tcgagaccgg cgccgtgtat ttcaccgctt cgatcgtcgg
     9301 cggcgtcaac ccgtcttcga gcaggatttt gatcccgaga tcgtcgttgt cgaagatcga
     9361 gttggcgtag atcgcgacga cggccgcttc ggcgctttcg accagcacgc cggcgccgcc
     9421 gttgaaggcg atcgtgttgg gaacgccccc caccgcgttg atcgcggtcg agtcggcaaa
     9481 gaaggtggcc cggatcccgt tgccggtgtt gggcagcggc gcttccccgc tggcgtcggt
     9541 tccgatcagg ttgccggaga tgctggcgtc gaagccttcc gcgttgatcc cgttgccttc
     9601 gttgccggag atcaggttgc cgcaggcgag gccgccgatc ctcacttttt cggcgtcgct
     9661 gctgatctgg atgccggacc cgttggctgc ggcggcaccg gtgacgtcga cgcccacgta
     9721 gttgccgcag gagacgcctt cgggctcgct ggagaagaag aggccggggc cgaccgcgcc
     9781 ggtgatcgcg agcccgcgga tttccgtttt cggcgcgtcg atgcggaggc cctgctcggc
     9841 cgccgccccg gaggcgtcga tttcgatcac cggcgtgcct tcgtagtttt cggcggtggt
     9901 gccgtcgat
//
LOCUS       DASRIG010000347         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1481878, whole genome shotgun sequence.
ACCESSION   DASRIG010000347 DASRIG010000000
VERSION     DASRIG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481878"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFB52_09400"
     CDS             <1..195
                     /locus_tag="VFB52_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06591.1"
                     /translation="ARRVEARRLLLFHHDPLHSDEFLDDFGATAKQAWQNLGRDATEL
                     ELGMEGSELSVGAPVPAVHA"
     gene            349..1827
                     /locus_tag="VFB52_09405"
     CDS             349..1827
                     /locus_tag="VFB52_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZO06592.1"
                     /translation="MKKRMGIGAGLALAALLALPGAAFAQRNGVGALGSQLDTTWVMV
                     ACVLVLFMQAGFLLLEIGFSRQKNVGAGVAKVLVNLAVVTLAWWAVGFGISSLTGNKI
                     FGTDGFFFHAGQEIGTGADAFVVGGGDTALMLFGMLFCAVSLAIVWGTTLERIRFAAY
                     AIYAAVFGAVIYPLVAHGVYGGGLLSDVGGKPVMDFAGSSVVHLTGAVGALAALLLLG
                     PRLGKYGADGRSRAIPGHSMPLVGLGVAILWVGWYGFNAGSTLGTSGNFMAEVALNTQ
                     LAAAAGVIGASVVTYAKTRSLDVGMAGNGAIAGLVGITAPSGFVEFWAAPIIGFVAGV
                     IVVFGVYAIDKRLDDPVGALSAHGLAGVWGTLAVGLFASDRLILDGAAPGIWYGIFGD
                     ASLGSTVGQFAVQAVGVAATFLLVFGLSYATFFAIKKTIGLRVSETEERAGLDISSHG
                     MFGYPEAFIPEEEYPGGPSPVPAPTPQPAAVTAMAADRPSEA"
     gene            1843..2211
                     /locus_tag="VFB52_09410"
     CDS             1843..2211
                     /locus_tag="VFB52_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZO06593.1"
                     /translation="MKKIEAYIRHEAFEPIRTELLDRGFPSLSIGEVKGSGRQKGIVE
                     TYRGSSLTVNVRPKLKLEVVVEDKDKSLVVETILRHARTGDIGDGKIFVIPVEEAIRI
                     RTGEEGEEVLQAHADAEIPA"
BASE COUNT          374 a          854 c          934 g          411 t
ORIGIN      
        1 gccaggcggg tggaggcacg gcggctcctg ctctttcacc acgatccgct ccactcggac
       61 gagttcctcg acgacttcgg cgccacggcg aagcaggcgt ggcagaacct cgggcgcgac
      121 gcgaccgagc tcgagctggg catggagggc tccgagctct cggttggggc ccctgtcccg
      181 gctgtccacg cctgagcggc cggcccttcg accaccggta caagggcggg cgcggaaact
      241 ttcgacaccg ggataacgac ttcgcacccc ccggcgccta ccgtcgctcg cgatcggatg
      301 tcatcgcgat caccgttcct tcgcgaacgg cggcaaggag ggaaaggaat gaagaagaga
      361 atgggcatcg gcgccggcct ggcgctcgct gccctcctgg cgttgccggg cgcggcgttc
      421 gcccagagaa acggggtcgg cgccctcggc tcacagctcg acaccacctg ggtgatggtg
      481 gcgtgcgtgc tggtgctctt catgcaggcg ggcttcctgc tgctcgagat cggcttctcg
      541 aggcagaaga acgtcggcgc gggagtggcg aaggtcctgg tcaacctcgc cgtcgtgacc
      601 ctcgcctggt gggcggtcgg cttcgggatc tcctccctga ccgggaacaa gatcttcggc
      661 accgacggct tcttcttcca cgccggacag gagatcggca ccggcgccga cgcgttcgtg
      721 gtcggcggcg gcgatacggc gctgatgctg ttcgggatgc tcttctgcgc cgtctcgctg
      781 gcgatcgtct gggggacgac gctcgagcgg atcaggttcg cggcctacgc gatctacgcc
      841 gcggtcttcg gcgcggtcat ctatccgctg gtggcgcacg gcgtgtacgg cggcggcctg
      901 ctgtccgacg tcggcggcaa gccggtgatg gacttcgccg gctcgtcggt cgtccatctc
      961 acgggcgccg tcggcgcgct cgcggcgctg ttgctgctcg gcccgcggct tggcaagtac
     1021 ggcgccgatg gccggtcgcg cgcgatcccg gggcactcga tgccgctggt cggcctcggc
     1081 gtcgcgatcc tgtgggtcgg ctggtacggc ttcaatgccg gctcgacgtt gggcacctcg
     1141 ggcaacttca tggctgaggt cgccctgaat acgcagctcg ccgccgcggc gggggtgatc
     1201 ggcgcctcgg tcgtgaccta cgcgaagacg cggtcgctcg acgtcggcat ggccggcaac
     1261 ggcgcgatcg ccggtctggt cggcatcacg gcgccgtcgg gcttcgtcga gttctgggcg
     1321 gcgccgatca tcggcttcgt cgccggcgta atcgtcgtct tcggcgtgta tgcgatcgac
     1381 aagcgcctcg acgacccggt cggcgcgctg tcggcgcacg gactcgccgg ggtctggggg
     1441 acgctcgcgg tcgggctgtt cgcctcggac cgcctgatcc tcgacggcgc ggcgcccggc
     1501 atctggtacg ggatcttcgg cgacgcgtcg ctcgggtcca cggtcgggca gttcgcggtg
     1561 caggccgtcg gcgtcgccgc cacgttcctg ctcgtgttcg ggctctccta tgcgacgttc
     1621 ttcgcgatca agaagacgat cgggctgcgg gtcagtgaga ccgaggagcg cgctgggctc
     1681 gacatcagct cgcacgggat gttcggctac cccgaggcgt tcatccccga ggaggagtac
     1741 ccaggcgggc ccagcccggt accggcgccg acaccgcagc cggccgcggt caccgcgatg
     1801 gccgcagatc gaccctcaga ggcctgaggg gaggtgatca agatgaagaa gatcgaggca
     1861 tacatccgcc acgaggcgtt cgagccgatc aggaccgagc tgctcgacag ggggtttccc
     1921 tccctgtcga tcggcgaggt caagggctcc gggcgccaga agggcatcgt cgagacctac
     1981 cgaggctcga gcctgaccgt gaacgtgcga ccgaagctca agctcgaggt cgtggtcgag
     2041 gacaaggaca agtccctggt cgtcgagacg atcctgcggc acgcgcgcac gggcgacatc
     2101 ggcgacggga agatcttcgt gatccccgtc gaggaggcga tccgcatccg caccggcgag
     2161 gagggcgagg aggtcctcca ggcgcacgcc gacgcggaga tcccggccta gggacgggcg
     2221 cgagcgggcc gcgcggtggt tgaggccgag ctgatgctcg cggccgccgg gcggcggccg
     2281 ggcgctcgga aacgccgcaa acgccgcgaa cgtcgcttca ggcaactgga atgcggaatt
     2341 gaccgcgcat acgactgtca actccgcaat cgcggtagag cgcgacaacg atcgtggagt
     2401 ttgcggcgtg ccgccggcgt cggccgctac ctgaccaacg cggttccacc cccaaccgac
     2461 gccgggtccg cgccacacgc gtgcgcgatt agttcggccg ggtcgagccg tctaccttga
     2521 cgacgagacg aacgacgcga ggaggggcca tgcccgccca ggaccgtgtg gag
//
LOCUS       DASRIG010000348         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3564754, whole genome shotgun sequence.
ACCESSION   DASRIG010000348 DASRIG010000000
VERSION     DASRIG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3564754"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFB52_09415"
     CDS             <1..431
                     /locus_tag="VFB52_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06594.1"
                     /translation="GIKLLAPDGFAQQSTIDLAGNASKGMFVSVPGLVPQDLSGPGKQ
                     LVNKLKQKSDGPVELFAPYAGQAASVVLSAIAASGERAGVLDAIRETKVRNGITGSFN
                     ITSSGDPSVGPITVSVAKSSFVPTRVVKPSAALVKAARQG"
     gene            457..1305
                     /locus_tag="VFB52_09420"
     CDS             457..1305
                     /locus_tag="VFB52_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZO06595.1"
                     /translation="MSSAPLQKDASVPVHYPPGMDARSTRVAEKLNPFMLVAAALTLP
                     TVAITESDPGGTMAAIAHVLNWVTWLAFLFEFVVMLAVVPDRKAWLRRHPLDAVIVFL
                     TPPVLPAGLQSLRVLRLLRLLRLFRLAQLSREAFSLEGVRYAALLAVLTAIGGASLFS
                     AFEHRQHTTFWEDLYWAITTMTTLGSNVEPTTTGGEIVSTVVLLVGIGFVALLTGSFA
                     QRFLAPEISELERELEEEEVSAEEIALHELKAVQQQLQTLEAAVQKMIEQQRGSPKGA
                     APPPSG"
     gene            complement(1302..>2711)
                     /locus_tag="VFB52_09425"
     CDS             complement(1302..>2711)
                     /locus_tag="VFB52_09425"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZO06596.1"
                     /translation="CLVTVDGARRKGKTAPIKPTVDEHMANHHALETIVVVRHTGIDC
                     PMSEGRDVFYDEILSAADPECPAEPMDAEHPLFILYTSGSTAKPKGILHTTGGYMTGV
                     TATHRYVFDLKPESDVYWCAADVGWITGHSYIVYGPLSNGATSVMWEGAPDYPHQGIW
                     WELVERYKATILYAAPTAIRTFIKWGAEIPAKFDLSSLRLLGSVGEPINPKAWLWYHK
                     VIGGERCPIVDTWWQTETGAIMITPLPGITATKPGSATSAFPGVEAQVVGESDGEPVS
                     EGQGLLMLERPWPSMLRTLYKEDERFVETYFSKFGPERYLVGDAARRDADGYFWVIGR
                     IDDVVNVSGHRLSTAEVESAIVAHPDVAEAAVIGQSDEDTGQAICAFVTLRGGTEPSE
                     ELVEGIRGEVAERIGKFARPKRIVWADDLPKTRSGKIMRRLLRDIAEGRALGDVTTLR
                     DPGVMAELEAKIAAEQDED"
BASE COUNT          393 a          962 c          929 g          427 t
ORIGIN      
        1 gcgggatcaa gctgctggcc ccggacgggt tcgcccagca gtcgacgatc gacctcgccg
       61 gcaacgcctc caaggggatg ttcgtcagcg ttcccggcct cgtgccgcag gacctgagcg
      121 gcccgggcaa gcaactggtc aacaagctga agcagaaaag cgacggcccg gtcgaactgt
      181 tcgcgcccta cgcgggccag gcggccagcg tcgtgctgag cgcgatcgcg gccagcggcg
      241 aacgcgccgg ggtgctcgac gcgatccgcg agaccaaggt ccgcaacggg atcaccggca
      301 gcttcaacat cacctccagc ggcgacccca gcgtgggtcc gatcaccgtc tcggtcgcca
      361 agagcagctt cgtgccgacc cgcgtggtca agccgagcgc cgccctggtc aaggccgccc
      421 gccagggctg agcgcagtcg cgtcgaatca ctcggggtga gttccgcccc gctccaaaaa
      481 gacgcctcgg tgccggtgca ctacccgccc gggatggacg cgcgcagcac ccgggtcgcc
      541 gagaagctga acccgttcat gctcgtcgcc gcggcgctga cgttgccgac cgtggcgatc
      601 accgagtccg accccggcgg cacgatggcg gcgatcgccc acgtgctcaa ctgggtcacc
      661 tggctcgcct tcctgttcga gttcgtggtg atgctcgccg tggtcccgga ccgcaaggcc
      721 tggctgcggc gccacccgct cgacgccgtg atcgtcttcc tcaccccgcc ggtcctgccc
      781 gccggcctgc agagcctgcg cgtgctgcga ctgctccggc tcctgcgcct cttccgcctc
      841 gcccagctct cgcgcgaggc cttctcgctg gagggcgtgc gctacgccgc cctgctcgcc
      901 gtgctcaccg cgattggcgg cgcctcgctg ttcagcgcct tcgagcaccg ccagcacacc
      961 accttctggg aagacctcta ctgggcgatc acgacgatga ccacgctcgg ctccaacgtc
     1021 gagccgacca cgaccggcgg tgagatcgtc tccaccgtgg tgctcctggt cgggatcggc
     1081 ttcgtcgccc tgctcaccgg ctccttcgcc cagcgcttcc tcgcccccga aatctccgag
     1141 ctcgagcgcg agctggagga agaggaggtc tcggccgagg agatcgcgct gcacgagctg
     1201 aaagcggtcc agcagcagct gcagacgctg gaggcggcgg tgcagaaaat gatcgagcag
     1261 cagcggggca gtcccaaagg ggccgcccct cctccgagcg gctagtcctc gtcctgctcc
     1321 gcggcgatct tcgcctcgag ttcagccatc accccggggt cgcgcagggt cgtcacgtcg
     1381 cccagcgccc ggccctcggc gatgtcgcgc aagagccggc gcatgatctt gccggagcgg
     1441 gtcttcggca ggtcgtcggc ccagacgatc cgcttcgggc gggcgaattt gccgatccgc
     1501 tcggcgactt cgccgcggat cccctcgacc agctcctcag agggctcggt gccgccgcgc
     1561 agggtgacga aggcgcagat cgcctggccg gtgtcctcgt cggactggcc gatcaccgcc
     1621 gcctcggcga cgtcggggtg ggcgacgatc gccgactcga cctccgcggt cgagagccgg
     1681 tggccggaga cgttgacgac gtcgtcgatc cggccgatca cccagaagta gccgtcggcg
     1741 tcgcggcggg cggcgtcgcc gaccaggtag cgctcgggcc cgaacttcga gaagtacgtc
     1801 tcgacgaagc gctcgtcctc cttgtagagg gtgcgcagca tcgagggcca gggacgttcc
     1861 aacatcagca ggccctggcc ttcggagacg ggctcgccgt cggactcgcc gaccacctgc
     1921 gcctcgaccc cggggaaggc gctcgtcgcc gagcccggct tggtcgcggt gatccccggc
     1981 agcggcgtga tcatgatcgc gccggtctcg gtctgccacc aggtgtcgac gatcgggcag
     2041 cgctcgcccc cgatcacctt gtggtaccag agccacgcct tcgggttgat cggctcaccg
     2101 accgagccga gcaggcgcag cgaggagagg tcgaacttcg ccgggatctc cgcgccccac
     2161 ttgatgaagg tgcggatcgc ggtcggcgcc gcgtagagga tcgtcgcctt gtagcgctcg
     2221 accagctccc accagatccc ctggtgcggg tagtccggcg ccccctccca catcacgctg
     2281 gtcgcgccgt tggagagcgg gccgtagacg atgtaggagt ggccggtgat ccagccgacg
     2341 tcggccgcgc accagtagac gtcggactcc ggcttgaggt cgaagacgta gcggtgggtc
     2401 gcggtaaccc cggtcatgta gccgccggtg gtgtggagga ttcccttcgg cttcgcggtt
     2461 gatccggagg tgtagaggat gaacagcggg tgctcggcgt ccatcggctc ggccgggcac
     2521 tcggggtcgg cggcggagag gatctcgtcg tagaagacgt cgcgcccctc gctcatcggg
     2581 caatcgatcc cggtgtggcg gacgacgacg atcgtctcca gggcgtggtg gttggccatg
     2641 tgctcgtcga cggtcggctt gatcggcgcg gtcttgccct tgcgccgcgc cccgtcgacg
     2701 gtgacgaggc a
//
LOCUS       DASRIG010000349         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3839651, whole genome shotgun sequence.
ACCESSION   DASRIG010000349 DASRIG010000000
VERSION     DASRIG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3839651"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..343
                     /locus_tag="VFB52_09430"
     CDS             131..343
                     /locus_tag="VFB52_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06597.1"
                     /translation="MNRGNGAAATTVTVLTVVGALVLFCLFFTAFLLVPFFVFIAAAV
                     ALVIAERNSKIKAEKPEAPADTGVEG"
     gene            340..2532
                     /locus_tag="VFB52_09435"
     CDS             340..2532
                     /locus_tag="VFB52_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06598.1"
                     /translation="MSQDPFRLVVRSPSGKRESMVTVSPTVVRLVPDRLRRELRIIPL
                     EYHEGMITVIAERVGDEAAENAILDATGFRPRWLLASPEEIARTLNATGPTEPGVESA
                     EGATFDAGYLVDHGLARLPRLGEELASRGLITEEELGEAIAEQERAGGRIGEILVLGG
                     ALEEHALLRVLADKQSLPTVDLSEFDPAKAPVDAIPEPLARSLRCVPIAMDDQYLFVA
                     VPDAPDRALAQEVEEHTHLKVRPFLAPASEIDALLRRIHGAEYSRVARFDLMERFPES
                     SAHKVLSGGQKGFLVAATLVAIAAAAFFFVPTAIVIFAVASIAYTANSGYKLISGYAA
                     LDHRYEFDATPEEMAALDERELPIYTVLVPLFREAAVLNQLVNSIESLDYPRRKLDVR
                     LLCEEDDDETIDAIKELNLPPHFETIIVPESQPQTKPKACNYGLLGAKGKYTVIYDAE
                     DRPEPDQLKKAVVMFERADESVACIQAKLNFFNQNTNLLTRWFSLEYSTLFDLILPGL
                     DARNDPIPLGGTSNHIRTDVLVDVGGWDPYNVTEDADLGIRLHKLGYRTIMMDSTTLE
                     EANKEIANWVRQRSRWIKGYIQTWLVYMRHPVRLLRDVGFKGFMSFQLMVGGAFIFLI
                     NPFFWLLTTIFFVTQSGLIQDLFPGWVYFLAAAQLLLGNFVFMYLAMAAASRRGYHGL
                     APYALSLPLYWGLMSLAAWKGFLQLFTNPFYWEKTEHGLDMSSPGTIV"
BASE COUNT          434 a          964 c          791 g          420 t
ORIGIN      
        1 cgctgatcac cggactgcgg cgcaccttgc cgctcgcgtt cgacatcagc gagtcggacg
       61 acagctcctt cacgatcgcg cttgcccagc cgcgggctga gcgcgagggc ggcgtggccg
      121 cccacgacgc ttgaaccgcg gcaacggggc tgcagcgacg acggtcaccg tgctgacggt
      181 ggtcggcgcg ctcgtcctgt tctgcctctt cttcacggcc ttcctgctgg tcccgttctt
      241 cgtcttcatc gcggccgcgg tcgcgctggt gatcgccgaa cgcaattcca agatcaaggc
      301 tgagaagccg gaggctcctg ccgataccgg agttgaagga tgagccagga tccctttcga
      361 ctcgtagttc gcagcccctc gggcaaacgc gagagcatgg tcaccgtctc cccgacggtg
      421 gtccgcctcg tgcccgaccg cctgcgccgc gagctgcgga tcatcccgct cgagtaccac
      481 gaggggatga tcacggtgat cgccgagcga gtcggcgacg aagccgccga gaacgcgatc
      541 ctcgacgcca ccgggttccg cccgcgctgg ctgctcgcct ccccggagga gatcgcccgc
      601 acccttaacg caaccggccc gaccgagccg ggagtcgagt ccgccgaggg cgccaccttc
      661 gacgccggtt atctggtcga ccacgggctc gcccgcctgc cccgcctcgg cgaggagctg
      721 gccagccgcg gcctgatcac cgaggaggag ctcggcgaag caattgccga acaggagcgc
      781 gccggcggcc ggatcggcga gatcctcgtg ctcggcgggg cgctggagga gcacgcgctg
      841 ctccgtgtcc tggccgacaa gcagagcctg ccgaccgtcg acctctccga gttcgacccc
      901 gcgaaagcgc cggtcgacgc gatccccgag ccgctcgcgc gcagcctgcg ctgcgtgccg
      961 atcgcgatgg acgaccagta cctgttcgtc gccgtccccg acgcgccgga ccgcgccctc
     1021 gcccaggagg tcgaagagca cacccacctg aaagtccgcc cgttcctcgc cccggcgagc
     1081 gagatcgacg cgctgctgcg ccgcatccac ggcgccgagt acagccgcgt cgcccgcttc
     1141 gacctgatgg agcgcttccc ggaatcgagc gcccacaaag tcctcagcgg tggccagaag
     1201 ggcttcctcg tcgccgccac cctggtcgcg atcgccgccg ccgcgttctt cttcgtcccg
     1261 accgcgatcg tgatcttcgc ggtcgcctcg atcgcctaca ccgccaactc gggctacaag
     1321 ctgatctccg gctacgccgc gctcgaccac cgctacgagt tcgacgccac ccccgaggag
     1381 atggccgccc tcgacgagcg cgagctgccg atctacaccg tgctcgtgcc gctgttccgc
     1441 gaggccgcgg tcctcaacca gctggtcaac tcgatcgaat cgctcgacta cccgcgccgc
     1501 aagctcgacg tgcggctgct ctgcgaggaa gacgacgacg agacgatcga cgcgatcaaa
     1561 gagctcaacc tgccgccgca cttcgaaacg atcatcgtcc cggaatcgca gccgcagacc
     1621 aagccgaagg cctgcaacta cggcctgctc ggcgccaagg gcaagtacac cgtgatctac
     1681 gacgccgagg accggcccga gcccgaccag ctgaagaaag cggtcgtgat gttcgagcgc
     1741 gccgacgaat cggtcgcctg catccaggcg aagctcaact tcttcaacca gaacacgaac
     1801 ctgctgaccc gctggttctc gctcgagtac agcaccctct tcgacctgat cctgccgggc
     1861 ctcgacgccc gtaacgaccc gatcccgctg ggcggcacct cgaaccacat ccgcaccgac
     1921 gtgctcgtcg acgtcggcgg ctgggacccc tacaacgtga ccgaggacgc cgacctcggc
     1981 atccgcctgc acaagctcgg ctaccgcacg atcatgatgg actcgaccac gctcgaggag
     2041 gcaaacaagg agatcgccaa ctgggtgcgc cagcgctcgc gctggatcaa gggatacatc
     2101 cagacctggc tggtctacat gcgccacccg gtgcggctgc tgcgcgacgt cggcttcaag
     2161 ggctttatga gcttccagct gatggtcggc ggcgccttca tcttcctgat caacccgttc
     2221 ttctggctgc tgacgacgat cttcttcgtc acccagtcgg ggctgatcca ggacctcttc
     2281 ccgggctggg tgtacttcct cgccgccgcc cagctgctgc tcggcaactt cgtcttcatg
     2341 tacctggcga tggcggccgc ttcacgccgc ggctaccacg ggctcgcccc ttacgcgctg
     2401 tcgctccccc tctactgggg cctgatgtcg ctggcggcct ggaagggctt cctgcagctg
     2461 ttcaccaacc cgttctactg ggagaagacc gagcacgggc tcgacatgtc ttctcccggc
     2521 acgatcgtct gacccgctcg cgggagagct gaccaatggc agagagcgca ctcgaactcg
     2581 atcacatcta cgactcgccg agcaaggag
//
LOCUS       DASRIG010000350         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1990210, whole genome shotgun sequence.
ACCESSION   DASRIG010000350 DASRIG010000000
VERSION     DASRIG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990210"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFB52_09440"
     CDS             <1..902
                     /locus_tag="VFB52_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO06599.1"
                     /translation="SEPHPGSGHGGPVVLGHEFVGRVVELGDGVGGLAVGDRVVSGAG
                     VSCGECSWCRAGRTNLCAHYYTLGFHRDGGLAEQVLTPASICVPVPDGCDDDAAAMAQ
                     PFAVALHGLNRAGLQDGETLAVIGTGGIGAFLVTAAAARGHGEVIAIDVAPDRLDTAA
                     VLGAAHLVDARSEDPVQAVREITGGIGADVVIDAAGAPGTLDQALRATRRGGRVLMVG
                     MPAEPPRIDVLDAILREVELTTTVAHVCGTDLPEALELLSTGKIAERVVERVIGLEQI
                     VAEGLEPLASGTARGKILVDVNG"
     gene            938..2335
                     /locus_tag="VFB52_09445"
     CDS             938..2335
                     /locus_tag="VFB52_09445"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO06600.1"
                     /translation="MAERMLIKAGTVLSMDDDLGVLTPGEVLIEDGVIAAVGTDLGVT
                     DAEVIDAPDSIVMPGFVDTHRHTWQAPLRLIASDWTLGHYLTGLHSGFSKHFRPEDTY
                     AGNLLGTLEALDSGITTLLDWSHNLATPAHADAAIEGLKDSGARAIFAHGAGAPQWQN
                     LPSTVPHSDDVKRLREEFFSTDDQLVTLAMALRGPQFATKETTLLDYQLAREVDTRIT
                     VHVGDGEWGRSRPIEWLRENDLLADDVTYVHCNELGDDELAMIADSGGTASVSADIEL
                     QMGHGWPATGRLLDAGIRPSLSIDVCSSNGGDMFGAMRSTIASQRGRDNGQAAEEGIS
                     LVEELRLGCRDVVEFATVEGARACGLDDKVGSLTPGKQADVILIDCDTLSLTPMNNPW
                     GAVVYAAHPGNVDTVIVAGKVVKRDGELVGVDVDRVKRLAVETRDYLMTQAEGDAAIA
                     DAELGGAWLPEQVRA"
     gene            2588..3721
                     /locus_tag="VFB52_09450"
     CDS             2588..3721
                     /locus_tag="VFB52_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06601.1"
                     /translation="MKLNALKSRVASPFVAIWIATALLFAISPLIASGSLSRDAIEAM
                     LPFAGILAIAAIGQTLVIQQGGLDLSVAGLFSLGAVLVVTIPGADSGQLPLALAVVLA
                     AGFGGGLINGLAVTRLGITPLVATLGSNAIFIGIVLEVTGGSIDFKATRNWQDFTADK
                     FLGIAVPALLALVLVIAVALFITRTVWGRRFVLVGSSPLAARAAGLAVERYQLLSYAI
                     AGVCYTFAGALLSGYLQSPPLLPGNEYLLPTIAAVVLGGTALGGGRGSVIATAGGVLF
                     LSQLETVISALGAETSVDYLVQGAIVALGMGLRNIPWRLLLARRGGDAGASPAPNPAS
                     QNSDSDGSGQGSPPPGVRSASGPSVGRALVSPNTEEDAIDVSK"
     gene            3708..>4863
                     /locus_tag="VFB52_09455"
     CDS             3708..>4863
                     /locus_tag="VFB52_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO06602.1"
                     /translation="MSVNRRARAKGPALLVALGVLFAALALSACGGGDDSSSSSSSSD
                     TGGSSTTASGGSGGANAWCGEDGVVLGIQDGGGLNAWSKESLAQVQAVAQECPAVEQE
                     IIVNAGFEPQKAVSGLQSMVAQGANAIVIIPDSGVCAEVPAMRQAMQRDAVVVPWAAD
                     PCGTVGTDYTKYIDWDTVAAGRAWAEWTIEQMGGKGKLLYEGGPAGNTVSANTAKGVL
                     EVVEENPGVELVEPISEEEWPVTNWDPAESRKITAALIAKHPQIDGLINDYGATAQTA
                     IQAFQAAGKPVPPTATTEANALACTWAEAKKDGEEFPLYTTSSRNWLGRYAAQTAIAD
                     ASGGEAPTLKNDGIIDLGEYENSVGGKEPKCDESFGPETYFSNEMTPAEMD"
BASE COUNT          760 a         1762 c         1637 g          704 t
ORIGIN      
        1 tgagcgagcc gcatcccggc agcggtcatg gcggtccggt cgtgctcggg cacgagttcg
       61 tcggccgtgt ggtcgagctc ggcgacgggg tcggtgggct ggcggtcggc gaccgggtcg
      121 tctccggcgc cggtgtcagc tgcggcgagt gctcctggtg ccgggccggc cgcaccaacc
      181 tctgcgccca ctactacacg ctcggtttcc accgcgacgg cggtctcgcc gagcaggttc
      241 tcaccccggc cagcatctgc gtccccgtcc ccgacgggtg cgacgacgac gccgccgcga
      301 tggcgcagcc gttcgccgtc gccctgcacg ggctgaaccg cgccggcctg caggacggcg
      361 agacgctggc ggtgatcggc accggcggca tcggcgcctt cctcgtcacc gccgccgcgg
      421 cccgcggcca cggcgaggtg atcgcgatcg acgtcgcccc cgaccggctc gacaccgccg
      481 ccgtgctcgg cgccgcccac ctggtcgacg cgcgcagcga ggacccggtc caggccgtgc
      541 gcgagatcac cggcggcatt ggcgccgacg tagtgatcga cgccgccggc gcccccggca
      601 ccctcgatca ggcgctgcgc gcgacccgcc gcggcggccg cgtgctgatg gtcgggatgc
      661 cggccgagcc gccgcggatc gacgtgctcg acgcgatcct gcgcgaggtc gagctgacga
      721 ccacggtcgc gcacgtctgc ggcaccgacc ttcccgaggc gctcgagctg ctgtccaccg
      781 gcaagatcgc cgagcgggtg gtcgagcgtg tgatcggctt ggagcaaatc gtcgccgagg
      841 gcctcgagcc cctggcatct ggaactgcgc ggggaaagat cctcgtcgac gtaaacggat
      901 aggccggcgg gtccggtccc gagaacagga gagagatatg gcagagcgga tgctgatcaa
      961 ggccgggacg gtcctcagca tggacgacga cctcggggtg ctcacgcccg gtgaggtgct
     1021 gatcgaggac ggcgtgatcg ccgccgtcgg caccgacctc ggcgtcaccg acgcggaggt
     1081 gatcgacgcc cccgactcga tcgtgatgcc cggcttcgtc gacacccacc gccacacctg
     1141 gcaggcgccg ctgcggctga tcgcctccga ctggacgctc ggccactacc tgaccggcct
     1201 gcactccggc ttcagcaagc acttccgccc cgaggacacc tacgccggca acctgctcgg
     1261 caccctcgag gcgctcgact ccgggatcac gaccctgctc gactggtccc acaacctcgc
     1321 cacgccggcc cacgccgacg ccgcgatcga gggcctcaag gactccggcg cgcgggcgat
     1381 cttcgcccac ggggccggcg ccccgcagtg gcagaacctg ccgagcacgg tcccgcactc
     1441 cgatgacgtc aagcgcctgc gcgaggagtt cttctccacc gacgaccagc tggtgacgct
     1501 ggcgatggcg ctgcgcggcc cgcagttcgc gaccaaagag acgaccctgc tcgactacca
     1561 gctggcgcgc gaagtcgaca cccggatcac cgtgcacgtc ggcgacggcg agtgggggag
     1621 atcgcgaccg atcgagtggc tgcgcgagaa cgacctgctc gccgacgacg tcacctacgt
     1681 ccactgcaac gaactcggcg acgacgagct cgcgatgatc gccgactccg gcggcaccgc
     1741 ctcggtctcg gccgacatcg agctgcagat gggccacggc tggcccgcga ccgggcggct
     1801 gctcgacgcc gggatccggc ccagtctctc gatcgacgtc tgctcctcca acggcggcga
     1861 catgttcggc gcgatgcgct cgacgatcgc ctcccagcgc ggccgcgaca atggccaggc
     1921 ggccgaggaa gggatctcgc tggtcgagga gctgcggctc ggctgccgcg acgtggtcga
     1981 gttcgcgacc gtcgagggtg cccgtgcctg cgggctcgac gacaaagtcg gctcgctgac
     2041 ccccggcaag caggccgacg tgatcctgat cgactgcgac acgctctcgc tgaccccgat
     2101 gaacaacccc tggggagcgg tcgtctacgc cgcccacccg ggcaacgtcg acaccgtgat
     2161 cgtcgccggc aaggtggtca aacgcgacgg cgagctggtc ggggtggacg tcgatcgcgt
     2221 caagcggctc gcggtcgaga cccgcgacta cctgatgacc caggccgagg gcgacgctgc
     2281 gatcgccgac gccgagctcg ggggcgcctg gctccccgag caggttcgcg cttaacgcgc
     2341 acccccttcc acaactcccc cagggactct cctcccgggt tgtagcgcga gcgccccgct
     2401 tcccccagcg gggcgctccg cgttgaggtc gcaattcctt ccggatagcg gaaagaagtg
     2461 cgacctcacc tggtgagaga cggctttaca agcgaaatgc gcctcgggtg ttagttatct
     2521 cttgacaccg tgttcagcaa catgctacga attcacggtg gcgcaacaag cgctgcacag
     2581 ggtggagatg aaattgaacg cactgaagag ccgcgtggcc tcgcctttcg tcgcgatctg
     2641 gatcgcgacc gcgctgttgt tcgcgatcag cccgctgatc gcctccggca gcctctcgcg
     2701 ggacgcgatc gaggcgatgc tgccgttcgc cggcatcctc gcgatcgccg cgatcggcca
     2761 gacgctggtg atccagcagg gcggtctcga cctctcggtc gccggcctct tctcgctcgg
     2821 cgcggtgctc gtcgtcacca tccccggcgc cgactcgggc cagctgccgc tggccctcgc
     2881 ggtcgtcctc gccgccggct tcggcggcgg cctgatcaac ggcctcgcgg tcacccgcct
     2941 cgggatcacg ccgctggtcg ccacgctcgg ctcgaacgcg atcttcatcg ggatcgtgct
     3001 cgaggtcacc ggcggcagca tcgacttcaa ggcgacccgc aactggcagg acttcaccgc
     3061 cgacaaattc ctcgggatcg ccgtcccggc gctcctggcc ctggtcctgg tgatcgccgt
     3121 cgcgctgttc atcacgcgca cggtctgggg acgccggttc gtcctcgtcg gctccagccc
     3181 gctcgccgcc cgcgccgccg gcctcgcggt cgagcgctac cagctcctga gctacgcgat
     3241 cgccggcgtc tgttacacgt tcgccggcgc cctgctctcc ggctacctgc agtcgccgcc
     3301 gctgctgccc ggcaacgaat acctgctgcc gacgatcgcc gcggtggtgc tcggcgggac
     3361 cgcgctcggt ggcggccgcg gcagcgtgat cgccaccgcc ggcggcgtgc tcttcctctc
     3421 gcagctggaa accgtgatct ccgcgctcgg cgccgagacc tcggtcgact acctcgtcca
     3481 gggcgcgatc gtcgccctcg gcatgggcct gcggaacatc ccctggcgcc tcctgctcgc
     3541 ccgtcgcggc ggcgacgccg gtgcctcccc ggcacccaac ccggcttccc aaaattcgga
     3601 cagcgatggc agtgggcaag ggagcccgcc gccgggcgtc cgatcggcga gcggaccgag
     3661 tgtcggccgc gcgcttgtca gtccaaacac agaggaggac gcaatcgatg tcagtaaata
     3721 gacgcgcgcg agcaaagggc ccggctctgc tggtcgccct cggcgtgctg ttcgccgcgc
     3781 tggcgctatc cgcctgcggc ggtggcgacg acagctcgtc gagctcgtcg agcagcgata
     3841 ccggcggttc cagcaccacc gcttccggcg gttccggcgg cgccaacgcc tggtgtggtg
     3901 aagacggcgt ggtcctgggg atccaggacg gcggcggtct caacgcctgg tccaaggagt
     3961 cgctggctca ggtccaggca gtcgcccagg aatgcccggc ggttgaacag gaaatcatcg
     4021 tcaacgccgg cttcgaaccg cagaaagcgg tctccggcct gcagagcatg gtcgcccagg
     4081 gcgccaacgc gatcgtgatc atccccgact ccggcgtctg cgccgaggtc ccggcgatgc
     4141 gccaggcgat gcagcgcgac gcggttgtgg tcccgtgggc cgccgacccg tgcggcaccg
     4201 tcggcaccga ctacaccaag tacatcgatt gggacaccgt cgccgcgggc cgtgcctggg
     4261 cggaatggac gatcgaacag atgggtggca aaggcaagct cctctacgag ggtggcccgg
     4321 ccggcaacac cgtctcggcc aacaccgcca agggcgtgtt ggaagtggtc gaagagaacc
     4381 cgggcgtcga actggtcgag ccgatctccg aagaagagtg gccggtcacc aactgggacc
     4441 cggcggaatc gcggaaaatc accgccgctc tgatcgccaa gcacccgcag atcgacggcc
     4501 tgatcaacga ctacggggcg accgcgcaga ccgcgatcca ggccttccag gccgctggca
     4561 agccggtccc gccgacggcg acgaccgagg ccaacgcgct cgcctgcacc tgggcggaag
     4621 cgaaaaaaga cggggaagaa ttccccctct acacgacgtc ctcgcgtaac tggctgggcc
     4681 gctacgccgc ccagaccgcg atcgccgatg cgtcgggtgg cgaagcgccg accctcaaaa
     4741 acgacgggat catcgatctc ggcgaatacg agaactcggt cggcggcaaa gaaccgaaat
     4801 gcgacgaatc gttcgggccg gaaacgtact tctcgaacga aatgacgccc gcggaaatgg
     4861 acg
//
LOCUS       DASRIG010000351         2885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3565699, whole genome shotgun sequence.
ACCESSION   DASRIG010000351 DASRIG010000000
VERSION     DASRIG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565699"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VFB52_09460"
     CDS             <1..1163
                     /locus_tag="VFB52_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06603.1"
                     /translation="SLATYQAQLFSTIKSSSLAGLPVVGPSMGNTNSDPTDISGSLEY
                     GNIHSYPNAEPPEWNLTRLLGTATPMSGSKPVMATETGYHNAMASDNGHRPASEAAAA
                     VYLPRLYLEYYRRGIVRTFSYELLDEFSDPSGVDLEDNFGLLRNDFSPKPSFTALSNL
                     IAILSDQGNSFTPGKLDFTVGGDKDELRQVLLQKSDGSYYLALWRAESVWSNKTLSPL
                     TAPSGKVELSFGQTITGAEEFAPNSSRQALRTLSTDGTSLNVNVGAQVVIVRLATDGT
                     SLGSIKVWVDEPAVEAGDPVYVEGKVAGTEGKPRKVKIQRWNKKSWKTVAKAKTKANG
                     RFKKQLRLLGGIGASRIRVIADRAKPSNQVKVRVLAADGGPKIALGNARKSA"
     gene            1177..2463
                     /locus_tag="VFB52_09465"
     CDS             1177..2463
                     /locus_tag="VFB52_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06604.1"
                     /translation="MPPLAAPFRAALALIALILALLLLAAPASAESVPARPAQAFVNS
                     LGINTHTYYQDTVYYQRFETIERRLHELGVRHIRENVVLHRPDQYRMLNRLADHGIDA
                     QLIAGDPRNGLGGMRALVDVLANRLDDAVAAIEGPNEYDLSGDRRWRPKLARYQRALY
                     RQVRATPELAGIPVVGPSIGPHHDPVDVPDLTGALDFGNVHSYPDGDAPEPDLDEWLG
                     AASRTSGPKRVMATETGYHTALRARDGQRPVSEAAMATYVPRVYLDYFARGIVRTFPY
                     ELADERPDPRRVEPEENFGLLRHDLSPKPAFTAVRNLVDLVEDPGPRFTAGSLDFTLG
                     GDTEELGSLLLQKRDGRFYLALWRTTSVWDADARARLSPGASPVRLTFATQPDRVTLF
                     LPNRSGTAQAELPLSAGATSLEVGPRVAVLEIDPAG"
     gene            complement(2470..>2885)
                     /locus_tag="VFB52_09470"
     CDS             complement(2470..>2885)
                     /locus_tag="VFB52_09470"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HZO06605.1"
                     /translation="FCGYNMGDYFAHWLKVGQTEGAELPRVFYVNWFRKDADGKFLWP
                     GFGENSRVLEWVFRRCEGKAEAIETPIGLLPPVDAIDTSGLEISAEAMAELLSVDTDL
                     VKQQLPQVREHLDKFGDKLPSEVSAQLEALEARLG"
BASE COUNT          457 a         1152 c          881 g          395 t
ORIGIN      
        1 ccagcctcgc cacctaccag gcccagctct tctccacgat caagagctcc tcgctcgccg
       61 gccttcccgt cgtcggcccc tcgatgggca acacgaacag cgacccgacc gacatctcgg
      121 gctcgctcga atacgggaac atccactcct acccgaacgc cgagccgccg gagtggaacc
      181 tcacccgcct gctcggcacc gcgaccccga tgtcgggctc caagccggtg atggcgaccg
      241 agaccggcta ccacaacgcg atggcctccg acaacggcca ccgccccgcc tccgaggccg
      301 ccgccgccgt ctacctgccc cgcctctacc tcgagtacta ccgccgcggg atcgtccgca
      361 ccttctccta cgagctgctc gacgagttct ccgacccctc cggcgtcgac ctcgaggaca
      421 acttcggcct gctgcgcaac gacttctccc cgaagccctc gttcaccgcg ctcagcaacc
      481 tgatcgcgat cctctccgac cagggcaact ccttcacccc cggcaagctc gacttcaccg
      541 tcggcggcga caaggacgag ctgcgccagg tgctcctgca gaagagcgac ggcagctact
      601 acctcgccct ctggcgcgcc gagagcgtct ggagcaacaa gaccctcagc cccctcaccg
      661 ccccctccgg caaggtcgag ctgagcttcg gccagacgat caccggggcc gaggaattcg
      721 cccccaacag ctcgcgccag gcgctgcgca cgctgagcac ggacggcacc tcgctgaacg
      781 tcaacgtcgg cgcccaagtc gtgatcgtcc gcctcgccac cgacggcacc tcgctcggca
      841 gcatcaaggt ctgggtcgac gagccggcgg tcgaggccgg tgacccggtc tacgtcgaag
      901 gcaaggtcgc cggcaccgaa ggcaagccgc gcaaggtgaa gatccagcgc tggaacaaga
      961 agagctggaa gacggtggcg aaggcgaaga ccaaggccaa cggccgcttc aaaaagcagc
     1021 tgcggctgct gggcgggatc ggcgcctcgc ggatccgcgt gatcgccgac cgggcgaagc
     1081 cgtcgaacca ggtcaaggtc cgcgtgctcg ccgccgacgg cggccccaag atcgcgctcg
     1141 gcaacgcccg caagtccgcc tagctagctt gggggcgtgc ccccgctcgc cgcccccttc
     1201 cgcgccgcgc tcgcgctgat cgcgctgatt cttgcgctgc tcctcctcgc cgccccggcg
     1261 agcgccgaga gcgtgcccgc ccgccccgcg caggcattcg tcaactcgct cgggatcaac
     1321 acccacacct actaccagga caccgtctac taccagcgct tcgagacgat cgagcggcgc
     1381 ctgcacgagc tcggcgtccg ccacatccgc gagaacgtcg tgctccaccg gcccgaccag
     1441 taccggatgc tgaaccggct cgccgaccac gggatcgacg cgcagctgat cgccggcgac
     1501 ccgcgcaacg ggctcggtgg gatgcgcgcg ctggtcgacg tgctcgccaa ccgcctcgac
     1561 gacgcggtcg ccgcgatcga gggcccgaac gagtacgacc tcagcggcga ccgccgctgg
     1621 cggcccaaac tcgcccgcta ccagcgtgcc ctctaccgcc aggtgcgagc gacgccggag
     1681 ctggccggta tccccgtcgt cggcccctcg atcggacctc atcacgaccc ggtcgacgtc
     1741 cccgacctga ccggcgcgct cgacttcggc aacgtccact cctaccccga cggcgacgcg
     1801 cccgagcccg acctggacga gtggctcggc gccgcctccc gcacctccgg ccccaagcgg
     1861 gtgatggcga ccgagaccgg gtaccacacg gcgctgcgtg cccgcgacgg ccagcgcccg
     1921 gtctccgaag cggcgatggc cacctacgtg ccgcgcgtct atctcgacta cttcgcccgc
     1981 ggcatcgtca ggacgttccc ctacgagctc gccgacgagc gtcccgaccc gcgccgggtc
     2041 gagccggagg agaacttcgg cctgctgcgc cacgacctct ccccgaagcc ggccttcacc
     2101 gcggtccgca acctcgtcga cctggtcgaa gaccccggcc cccgcttcac cgccggcagc
     2161 ctcgacttca ccctcggcgg cgacaccgag gagctgggct ccctgctgct ccagaaacgc
     2221 gacgggcgct tctacctggc gctctggcgc acgaccagcg tctgggacgc cgacgcgcgc
     2281 gcccgcttga gccccggcgc ctccccggtg cgcctgacct tcgcgaccca gcccgatcgc
     2341 gtcaccctct tcctgccgaa ccgctccggc acggcgcagg ccgagctgcc gctcagcgcg
     2401 ggcgcgacct cgctcgaggt cggcccgcgc gtcgcggtgc tcgaaatcga tcccgccggc
     2461 taggcggggt tagccgaggc gcgcttccag tgcctcgagc tgcgcgctga cctcgctcgg
     2521 cagtttgtcg ccgaacttgt cgaggtgctc gcggacctgg ggcagctgct gcttgaccag
     2581 gtcggtgtca accgagagca gctccgccat cgcctcggcg gagatctcca ggcccgaggt
     2641 gtcgatcgcg tcgaccggcg gcagcagccc gatcggggtc tcgatcgcct ccgccttgcc
     2701 ctcgcagcgg cggaagaccc actcgagcac gcggctgttc tcgccgaacc cgggccagag
     2761 gaacttgccg tcggcgtctt tgcggaacca gttgacgtag aagacacgcg gcagctcggc
     2821 gccctccgtc tggccaacct tcagccagtg ggcgaagtag tcgcccatgt tgtagccgca
     2881 gaacg
//
LOCUS       DASRIG010000352         9346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_945695, whole genome shotgun sequence.
ACCESSION   DASRIG010000352 DASRIG010000000
VERSION     DASRIG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945695"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFB52_09475"
     CDS             <1..281
                     /locus_tag="VFB52_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO06606.1"
                     /translation="KVTLPLTMPGIVAGVLLTFIPAFGDYINAQLLGGPQQAMVGNVI
                     QNLYLNQRNYPEAAALSFLMMALILIVVSLYIRFAGIGAFMGDEEEKS"
     gene            281..1123
                     /locus_tag="VFB52_09480"
     CDS             281..1123
                     /locus_tag="VFB52_09480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06607.1"
                     /translation="MEAFSRAWAWLRHQALPIYAALATAYLLIPIAVIAIFSFGATPK
                     GKLTFALDEGFTLEYWETMFDIEALNEALLTSVELAALATAIATAIGTAMAIALVRHQ
                     FKGRRAANLLIVIPMATPEVVIGASLLSMFIYANFALGFSTLLIAHVMFCISFVVVVV
                     RSRLIGFDRKLEEAAADLGATPFTTFRLVTLPLILPGVIAAAMLAFALSIDDFVISNF
                     NSGTTVTFPLYVYGVSLRGIPVQVNAIATLLFLVASAAAIAVMLQQRRAERMSALPAD
                     VKAN"
     gene            complement(1097..2494)
                     /locus_tag="VFB52_09485"
     CDS             complement(1097..2494)
                     /locus_tag="VFB52_09485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO06608.1"
                     /translation="MEHTATGYWLAEAGEPAPAPPLAESLDADVLIVGGGYTGLWSAW
                     YAKQLEPAARVVLLEAGVCGHGPSGRNGGFCNAMWWSLPSMRDRWGAAPALAVARAAE
                     RGVERIGAFCAEQGVEAWWHPTDYLHVSTVAAHDGGWRDVIETCRELGVEGVVRELSP
                     EEVSARCASPAFRGGIAVGGSATVQPARLALGLRERLLAAGVEIYEGSPVRGFREGED
                     GIEARTPGGSVRTPRAVLAIGPAAKGRRGPLRGRLSIASSHIVLTEPVPELLAEIGWS
                     GGECITDLRALLHYFRTTPDGRIAFGWGGGRIAFGARTRGRAELDREVVDAVRGHLLD
                     YFPGLAGKQITHAWGGPIDASPQHLPMVLSQRHGRAFAAAGYTGNGVGPSNMVGRTLA
                     ALALDRRDEETALAFVDPKVPRVPPEPFHWLGGEAIRLGILAKEEAEMAGRVPGKLAS
                     ALAAVPKLIGFHIGR"
     gene            2528..3997
                     /locus_tag="VFB52_09490"
     CDS             2528..3997
                     /locus_tag="VFB52_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZO06609.1"
                     /translation="MPDHGQDATPFVDALLAYAAREPGRFQVPGHKGGAGADPKMREL
                     VGEAGLRNDIPSVTEGVDIGPEPTPFQQAQLLAAEAWGAERTWFLMNGASQGNHVACL
                     ALAHMGSEVVVQRNVHSSVIDGLVLSGLRPTFVSPQLDPELGIAHGLTPDSLDAGLAA
                     TPNAVAAMVVSPTYFGACADVAALAEVAHARGVPLVADEAWGAHLHFSDELPPDALAC
                     GADLVTSSTHKIVGSLTQAAMLHLGAGGRLDPAVVDRCVSLVETTSPSGLLTGSLDAA
                     RRQAAVHGGELLGETLAAVARTRAAVAEIPGLAVLDAALVGQPGIAGWDPLRLAIDVR
                     GTGSTGYKLAREIFYSQGIDLELYSENVVVAIFGLGEPAGPAGERLVAALREAVAELE
                     AEPGPPQEKLAPPPPWGELVLTPREAFLGPQEVVPFDDAAGRIAAEGLAAYPPGIPNV
                     LPGERLTAETLAYIRESVAHGGYVRGGSDRQLKTLRVAR"
     gene            4009..5085
                     /locus_tag="VFB52_09495"
     CDS             4009..5085
                     /locus_tag="VFB52_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06610.1"
                     /translation="MASYDAIRDLPLEIESCSFEGLELVLGEFERLTTVIKFSGGGHE
                     GIGEDVTYDAVDHVAQQSAGPQDGLTGTFTFDEFSQRLAGIDLFAAGGPEREMSVDYR
                     RWAFESAALDLALRQAGTNLAAILGREPRPLNFVSSMRLAGFEEGAKSSIEPLRERLA
                     VYPTLRFKLDPFNDWDDELIDALVATGAVDSLDLKGFYKGTPVDVITDPELYGKLIEK
                     FPDAWLEDPDVTAETKPLLDPVSERVTWDAPIHSIADIEAMPWSPPKTVNVKPSRFGP
                     ISNLFAAYDYCEERGIGAYGGGQTELGPGRGQIQYLASIFHPDTPNDVAPSSYNDPAQ
                     ATKPGLPSSPLEPSIDELGFRWRA"
     gene            5142..5816
                     /locus_tag="VFB52_09500"
     CDS             5142..5816
                     /locus_tag="VFB52_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06611.1"
                     /translation="MRILVVDDEHAVRDALRRALRLGGYDVQIAPGGEVALDLVRDAR
                     PDAIVLDVLMPGMDGLELCRRLRAGGDRTPVLMLTARDAIGDRVLGLDAGADDYLVKP
                     FALEELQARLRALLRRSGAEEPGTLAFAGVTLDLGSHEVEREGRRIELTRTEFLLLEL
                     FLRNPRQVLERGTIFDRVWGYDFGPSSNSLEVYIGYLRRKLEADGEPRLVHTVRGVGY
                     VLREPR"
     gene            5813..7261
                     /locus_tag="VFB52_09505"
     CDS             5813..7261
                     /locus_tag="VFB52_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO06612.1"
                     /translation="MSFRRRIVIATGVAVSVAIVLASVAIYVLVRGELRNQVDSELDR
                     LSGAVFAIRIDGQGGNNVAIGKVLKERLPGVKGEAKAFPSKGLKSVEVRLPKDALGSS
                     VGYAQFVNAKGRVIGPPRDDVRLPVTDRTLEVARGEAPAFYSDAEVNGIHVRMLTSRV
                     GGERAVQAVRSLEGVDQTLERLLLVLVVVCVGGIGLALGLGLLVGRTALRPVRQLTEA
                     AEHVATTRDLSRRIDARGRDELARLARSFNTMLAALERSLGAQRQLVADASHELRTPL
                     TSLRTNVEVLADVERLRPAERELLLADVVAQLEELSDLVGDLIDLAREEEAAPVQEDV
                     RLDDLVAAAVARAERNHPGLRIEVELEPCVVRATPSRLDRAVANLIDNARKWSAPGEP
                     VEVVLRDGELAVRDHGPGFADADMPHVFDRFYRSPAARGRPGSGLGLAIVRQVAESQG
                     GAVSAANAPGGGALVRLWLPAAAPATVPALDL"
     gene            7339..7896
                     /locus_tag="VFB52_09510"
     CDS             7339..7896
                     /locus_tag="VFB52_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06613.1"
                     /translation="MTRKDWAVAVAIALCAIGLIACGGGGGSASGSDAAAAGEIDEGA
                     MEEGRLEMAECMREHGVDVPDPVAGEKGLMLKKGPGGSGVDLDDPATEEALEACEDEV
                     EFQPPEATPEQEEEMQENMLAFAECMREHGVDMPDPEFEEGGKVKMRIGGPDGVDLDQ
                     PAVEAAQEACQEELPEGGFGFKAGP"
     gene            7893..9200
                     /locus_tag="VFB52_09515"
     CDS             7893..9200
                     /locus_tag="VFB52_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZO06614.1"
                     /translation="MKRALKAARRLGRKRALLAAALLALVAGGAAVALAGGSEEGAGG
                     ESERGASAEGTGKVERRTLAEQLTAGGTIGYAGESTVLARLTGTVTALPSVGDKVEHG
                     EQLYALAGEPVLLMYGEVPAYRTLAEGIEDGPDVEQLEQNLAALGYEPGTVDEEFSSS
                     TASAISAWQEETGLEATGEVELGRVAFLPGPRRVTQVEATLGEAIGSGSGGNGLVSYE
                     PPIGEAEEAPEEEEPEPQPEKPETEETEEPAPEERTAAPEASASGGGGAEPTDEGESD
                     PSSVPILKTSSTRHIVSVELDPDQQSVAHRGQKVEVLLPGGEEADGKVQRIAVAESSS
                     ESPGEESEPGVEVTVSLLGKSKVPALDGAAVSVLFTQNVREDVLSVPLTALVAIGSER
                     FAVVAIEGTARRQIVVTPGLAADGYVEVEGKGLREGMLVETGQ"
     gene            9251..>9346
                     /locus_tag="VFB52_09520"
     CDS             9251..>9346
                     /locus_tag="VFB52_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06615.1"
                     /translation="MEALRGVSLAIGAGELTAIVGPSGSGKSTLLH"
BASE COUNT         1379 a         3364 c         3352 g         1251 t
ORIGIN      
        1 tgaaggtgac gctgccgctg acgatgccgg gaatcgtcgc cggcgtgctg ctcaccttca
       61 tccccgcctt cggcgactac atcaacgcgc aactgctcgg cgggccccag caggcgatgg
      121 tcggcaacgt gatccagaac ctctacctga accagcgcaa ctacccggag gcggcggcgc
      181 tctccttcct gatgatggcg ctgatcctga tcgtcgtctc gctctacatc cggttcgcgg
      241 ggatcggcgc cttcatgggt gacgaggagg agaaatcctg atggaagcct tctcccgagc
      301 ctgggcctgg ctgcgccacc aggcgctgcc gatctacgcg gcgctggcca ccgcctacct
      361 gctgatcccg atcgccgtga tcgcgatctt ctccttcggc gcgacgccga aagggaaact
      421 caccttcgcg ctcgacgaag gcttcaccct cgaatactgg gaaacgatgt tcgacatcga
      481 agcgctgaac gaagcgctgc tgaccagtgt cgagctggcg gcgctggcaa ccgcgatcgc
      541 gaccgcgatc gggacggcga tggcgatcgc attggtccgc caccagttca agggccggcg
      601 ggccgccaac ctgctgatcg tgatcccgat ggcgacgccg gaggtggtga tcggcgcctc
      661 gctgctctcg atgttcatct acgccaactt cgcgctcggc ttctccaccc tgctgatcgc
      721 ccacgtgatg ttctgcatca gcttcgtcgt ggtcgtcgtg cgctcgcggc tgatcgggtt
      781 cgaccgcaag ctcgaggagg cggccgcgga cctcggcgcg acccccttca ccaccttccg
      841 cctcgtcacc ttgccgctga tcctgcccgg agtcatcgcc gccgcgatgc ttgccttcgc
      901 gctctcgatc gacgacttcg tgatctccaa cttcaactcg gggacgacgg tgaccttccc
      961 gctctacgtc tatggcgtca gcctgcgcgg catcccggtc caggtcaacg cgatcgccac
     1021 cctgctcttc ctggtcgcct cggccgccgc gatcgcggtg atgttgcagc agcgccgggc
     1081 cgagcggatg tcggccctac cggccgatgt gaaagccaat tagcttcggc acggcggcga
     1141 gcgcggaggc gagcttgccg ggcacgcggc cggccatctc cgcctcctcc ttggcgagga
     1201 tgccgagccg gatcgcctcg ccgccgagcc agtggaaggg ctcgggtggg acgcgcggga
     1261 ctttcgggtc gacgaaggcg agcgcggtct cctcgtcgcg gcggtcgagc gcgagcgcgg
     1321 cgagcgtgcg gccgaccatg ttcgagggcc cgaccccgtt gccggtgtag ccggcggcgg
     1381 cgaaggcgcg gccgtggcgc tggctcagca ccatcggcag gtgctggggc gaggcgtcga
     1441 tcgggccgcc ccaggcgtgg gtgatctgct tgccggccag ccccgggaag tagtcgagca
     1501 ggtgtccgcg gaccgcgtcg acgacctctc ggtcgagctc ggcgcggccg cgggtgcggg
     1561 cgccgaaggc gatccggccg ccgccccagc caaaggcgat gcggccgtcc ggggtggtgc
     1621 ggaagtagtg caggagggcg cgcaggtcgg tgatgcactc gcccccgctc cagccgatct
     1681 cggccagcag ctcgggcacc ggctcggtca ggacgatgtg cgaggaggcg atcgagaggc
     1741 ggccacgcag cgggccacgc cggcccttgg cggcggggcc gatcgccagc accgcacgcg
     1801 gggtgcgcac gctgccgcca ggggtgcgcg cctcgatccc gtcctcgcct tcgcggaagc
     1861 cgcgcaccgg cgagccctcg tagatctcga ccccggcggc gagcagccgc tcgcgcaggc
     1921 cgagcgccag ccgcgccggc tgcacggtcg ccgagccgcc gaccgcgatc ccgccgcgga
     1981 aggcgggcga ggcgcagcgg gcggagactt cctccgggga gagctcgcgc accacgccct
     2041 cgaccccgag ctcgcggcag gtctcgatca cgtcgcgcca gccgccgtcg tgggcggcga
     2101 cggtcgagac gtgcaggtag tcggtcgggt gccaccaggc ctcgacgccc tgctcggcgc
     2161 agaaggcgcc gatccgctcg accccccgct cggccgcgcg ggcgacggcg agcgccggcg
     2221 ccgcgcccca gcggtcgcgc atcgagggca gcgaccacca catcgcgttg cagaagccac
     2281 cgttgcggcc gctcggcccg tgcccgcaga cgccggcctc gagcagcacc acccgcgccg
     2341 ccggctccag ctgcttcgcg taccaggcgc tccacaaccc ggtgtagccg ccgccgacga
     2401 tcagcacgtc ggcatcgagc gactccgcga gcggcggtgc cggcgccggc tcgcccgcct
     2461 ccgccaacca gtagccggtt gccgtgtgct ccattgcccg acagtttccc ctacgtagac
     2521 tcgaaccgtg cccgatcacg gtcaggacgc gactcccttc gtcgatgccc tgctcgccta
     2581 cgccgcgcgc gagccgggac gcttccaggt gcccggacac aagggcggcg ccggcgccga
     2641 cccgaagatg cgcgagctgg tcggcgaggc cggcctgcgc aacgacatcc cctcggtcac
     2701 cgagggcgtc gacatcggtc ccgaaccgac cccgttccaa caggcgcagc tgctcgcggc
     2761 cgaggcctgg ggggcggagc gcacctggtt cctgatgaac ggcgcctcgc agggcaacca
     2821 cgtcgcctgc ctggcgctcg cccacatggg cagcgaggtc gtcgtccagc gcaacgtcca
     2881 ctcctcggtg atcgacgggc tggtgctgag cgggctgcgc ccgaccttcg tctcgccgca
     2941 gctcgacccg gagctgggga tcgcgcacgg gctcaccccc gactcgctcg acgccgggct
     3001 cgcggcgacg ccgaacgcgg tcgcggcgat ggtcgtctcg ccgacctact tcggcgcctg
     3061 cgccgacgtc gccgcgctcg ccgaggtcgc ccacgcgcgc ggcgtgccgc tggtcgccga
     3121 cgaggcctgg ggcgcccacc tccacttcag cgatgagctg ccgccggacg cgcttgcctg
     3181 cggcgctgac ctcgtcacct cctcgaccca caagatcgtc ggcagcctca cccaggcggc
     3241 gatgctgcac ctcggcgcgg gcgggcgcct cgacccggcg gtggtcgacc gctgcgtcag
     3301 cctggtcgaa accaccagcc ccagcggcct gctgaccggc tcgctcgacg cggcccggcg
     3361 gcaggcggcg gtgcacggcg gcgagctgct cggggagacg ctggccgcgg tcgcgcgcac
     3421 ccgcgccgcg gtcgccgaga tccccggcct cgcggtgctc gacgcggccc tagtcggaca
     3481 gcccgggatc gccggctggg atccgctgcg gctcgcgatc gacgtgcgcg gcaccggctc
     3541 gaccggctac aagctggccc gcgagatctt ctactcgcag gggatcgacc tcgagctcta
     3601 ctcggagaac gtcgtggtcg cgatcttcgg gctcggcgag ccggccgggc cggcgggcga
     3661 gcggctggtc gcggcgctgc gcgaggcggt cgccgaactg gaagccgagc cggggccgcc
     3721 gcaggagaag ctggcgccgc cgccgccctg gggcgagctg gtgctgaccc cgcgcgaggc
     3781 cttcctcggc ccgcaggagg tcgtcccctt cgacgatgcc gccgggcgga tcgccgccga
     3841 gggcctcgcc gcctacccgc ccgggatccc caacgtgctg cccggcgagc ggctgaccgc
     3901 cgagaccctc gcctacatcc gcgagagcgt cgcccacggc ggctacgtcc gcggcggctc
     3961 cgaccgccag ctcaagacgc tgcgcgtcgc ccgctagggt ctctgcggat ggccagctac
     4021 gacgcgattc gcgacctgcc gctcgagatc gagtcctgct cgttcgaagg gctggaactg
     4081 gtgctggggg agttcgaacg gctgaccacg gtgatcaagt tcagcggcgg cggccacgag
     4141 gggatcggcg aagacgtcac ctacgacgcc gtcgaccacg tcgcccagca gagcgccggg
     4201 ccgcaggacg ggctgaccgg gacgttcacc ttcgacgagt tctcgcagcg gctggccggc
     4261 atcgatctct tcgccgccgg cggtccggag cgcgagatgt cggtcgacta ccgccgctgg
     4321 gccttcgagt cggcggcgct cgacctggcg ctgcgccagg cgggcaccaa cctggcggcg
     4381 atcctcggcc gcgagccccg tccgctgaac ttcgtcagct cgatgcggct cgccggcttc
     4441 gaagagggcg ccaagtcctc gatcgagccg ctgcgcgaac ggctcgccgt ctaccccacc
     4501 ctgcgcttca agctcgaccc gttcaacgac tgggacgacg agctgatcga cgccctggtc
     4561 gcgaccggcg cggtcgactc gctcgacctc aagggcttct acaaaggcac cccggtcgac
     4621 gtgatcaccg accccgagct atacgggaag ctgatcgaga agttccccga cgcctggctc
     4681 gaggacccag acgtaacggc cgagaccaag ccccttctcg atccggtatc ggagcgagtc
     4741 acctgggacg cgccgatcca ctcgatcgcc gacatcgagg cgatgccgtg gtcgccgccg
     4801 aagacggtca acgtgaagcc ctcgcgcttc ggcccgatct ctaacctgtt cgccgcctac
     4861 gactactgcg aggagcgcgg gatcggcgcc tacggcggcg gccagacgga gctcggaccc
     4921 gggcgcgggc agattcagta cctggcctcg atcttccacc ccgacactcc caacgacgtc
     4981 gcccccagca gttacaacga ccccgcccag gcgaccaagc cgggattgcc ctcgagcccg
     5041 ctggagccgt cgatcgacga gctcgggttc cgctggcgcg cctgatccgt cccgtccggg
     5101 tctcttagga aaaactcagg cgggggtgtg accctgcgcc gatgcgcatc ctggtcgtgg
     5161 acgacgagca cgccgtccgt gacgcgctgc ggcgggcgct gcggctcggc ggctacgacg
     5221 tccagatcgc cccgggcggc gaagtcgccc tcgacctggt ccgcgacgcc cgccccgacg
     5281 cgatcgtgct cgacgtgctg atgcccggga tggacgggct cgaactgtgc cggcgcctgc
     5341 gcgccggcgg cgaccgtacg ccggtgctga tgctgaccgc gcgcgacgcg atcggcgacc
     5401 gcgtgctcgg cctcgacgcc ggcgccgacg actacctggt caagccgttc gcgctcgagg
     5461 agctgcaggc gcggctgcga gcgctgctgc gccgcagcgg cgccgaggag ccgggcacgc
     5521 tggccttcgc cggggtgaca ctcgacctcg gcagccacga ggtcgagcgg gaggggcgcc
     5581 ggatcgagct gacccggacc gagttcctgc tgctcgagct gttcctgcgc aacccgcgcc
     5641 aggtgctcga gcgggggacg atcttcgacc gcgtctgggg ctacgacttc ggccccagct
     5701 cgaactcgct cgaggtctac atcggctacc tgcggcgcaa gctggaggcc gacggtgagc
     5761 cgcgcctcgt ccacaccgtg cgcggggtcg gctacgtgct gcgggagccg cggtgagctt
     5821 ccggcgccgg atcgtgatcg ccaccggggt cgcggtctcg gtcgcgatcg tgctcgcctc
     5881 ggtggcgatc tacgtgctcg tgcgcggcga gctgcgcaac caggtcgact ccgaactcga
     5941 ccgcctctcc ggcgccgtgt tcgcgatccg catcgacggc cagggcggga acaacgtggc
     6001 gatcggcaaa gtgctgaaag agcgcctgcc gggggtcaaa ggcgaggcga aagcgttccc
     6061 ctccaaaggg ttgaaatcag tcgaggtgcg gctgcccaaa gacgcgctcg ggagcagcgt
     6121 cggctacgcc cagttcgtca atgcgaaggg ccgggtgatc gggccgccgc gcgacgacgt
     6181 gcggctgccg gtgaccgacc ggacgctgga agtcgcgcgc ggcgaagctc cggccttcta
     6241 cagcgacgcc gaggtcaacg gcatccacgt ccgcatgctg accagccggg tcggcggcga
     6301 acgcgcggtg caggcggtgc gctcgctgga gggcgtcgac cagacgctcg agcggctgct
     6361 gctggtcctg gtcgtggtct gcgtcggcgg gatcgggctg gcgctcgggc tcggtctgct
     6421 ggtcgggcgc accgccctgc ggccggttcg ccagctgacc gaagcggccg agcacgtcgc
     6481 caccacccgc gacctcagcc gccgcatcga cgcccgcggg cgcgacgagc tggcgcggct
     6541 ggcgcgcagc ttcaacacga tgctggcggc gctcgaacgc tcgctcggcg cccagcgcca
     6601 gctcgtcgcc gacgcctcgc acgagctgcg cacgccgctg accagcctgc gcaccaacgt
     6661 cgaggtgctc gccgacgtcg agcggctgcg cccggccgaa cgggagctgc tgctcgccga
     6721 cgtggtcgcc caactcgagg aactgagcga cctggtcggc gacctgatcg acctcgcgcg
     6781 cgaagaggaa gcggcgccgg tgcaggagga cgtgcgcctc gacgacctgg tcgcggcggc
     6841 ggtggcgcgg gccgagcgca atcacccggg gctgcggatc gaggtcgagc tggaaccctg
     6901 cgtggtccgc gccaccccct cgcgcctgga ccgcgcggtc gccaacctga tcgacaacgc
     6961 gcgcaagtgg agtgcgccgg gcgagccggt cgaggtcgtg ctgcgcgacg gcgagctggc
     7021 ggtgcgcgac cacgggcccg gcttcgccga cgccgacatg ccccacgtct tcgaccgctt
     7081 ctaccgctcg ccggcggcgc gtgggcggcc cggcagcggg ctcgggctgg cgatcgtgcg
     7141 ccaggtcgcc gagtcccagg gcggcgcggt cagcgccgcc aacgcgcccg ggggcggcgc
     7201 cctggtgcgg ctctggctgc cggcggcggc gcccgccacg gtcccggcgc tcgatcttta
     7261 gacagctcct aacgccctct catcttgctc tcagccggcg acggcttgat gcctgccgtc
     7321 aaccgaaagg agctggagat gacgaggaaa gactgggcgg tggcggtggc gatcgcactc
     7381 tgcgcgatcg ggctgatcgc ctgcgggggc ggcggcggat cggcctccgg aagcgacgcg
     7441 gcggccgcgg gcgagatcga cgagggcgcg atggaagaag ggcgcctgga aatggccgaa
     7501 tgcatgcgcg agcacggcgt cgacgtcccc gatccggtcg cgggcgagaa aggcctgatg
     7561 ctcaaaaaag ggcccggcgg aagcggcgtc gacctcgacg acccggcgac cgaggaagcg
     7621 ctggaagcgt gcgaggacga ggtcgaattc cagcccccgg aggcgacccc cgagcaggaa
     7681 gaagagatgc aggaaaacat gctcgccttc gccgaatgca tgcgcgagca cggcgtcgac
     7741 atgcccgatc ccgaattcga ggagggcggc aaagtgaaaa tgcggatcgg cggtcctgac
     7801 ggggtcgacc tcgaccagcc cgcggtcgaa gcggcgcagg aagcctgcca ggaagagctg
     7861 cccgaaggcg gcttcggatt caaagccggg ccgtgaagcg agcgctgaag gcggcccgcc
     7921 gcctcggcag gaagcgggcg ctgctcgccg ccgcgctgct ggcgctggtc gccggcggtg
     7981 cggcggtggc gctcgccggc ggctccgagg aaggcgcggg cggcgagagc gagagggggg
     8041 cctcggccga gggcaccggc aaggtcgagc gccgcacgct ggccgaacag ctcaccgccg
     8101 gcggcacgat cggctacgcg ggcgagtcaa ccgtgctggc ccgcctgacc ggcacggtga
     8161 cggcgctgcc cagcgtcggc gacaaggtcg agcacggcga gcagctctac gcgctggccg
     8221 gcgaaccggt gctgctgatg tacggcgagg tgccggcgta ccgcacgctc gccgaaggga
     8281 tcgaagacgg ccccgacgtc gaacagctgg agcagaacct ggcggcactc ggctacgaac
     8341 ccgggaccgt cgacgaagaa ttcagttctt cgaccgcatc cgcgatcagc gcctggcagg
     8401 aagagaccgg gcttgaagcg accggcgaag tcgaactggg tcgggtcgcc ttcctccccg
     8461 gcccgcgccg cgtcacccag gtcgaagcga cgctcggcga agcgatcggc tccggcagcg
     8521 gtggaaacgg gctcgtctcc tatgaaccgc cgattggcga agcggaagaa gcgcccgaag
     8581 aggaagaacc cgaaccgcaa ccagagaaac cagaaaccga agagacggaa gaaccggcac
     8641 ccgaagagcg caccgccgct ccggaagcct cggccagtgg cgggggcggc gccgaaccga
     8701 ccgacgaagg cgaatccgac ccctcctcgg tgccgatcct gaagaccagc tcgacccggc
     8761 acatagtcag cgtcgaactc gaccccgacc agcagtcggt cgcccaccgc gggcagaagg
     8821 tcgaggtgct gctgcccggc ggcgaggaag ccgacggcaa ggtgcagcgg atcgcggtcg
     8881 ccgaaagcag ctccgaatct ccgggcgaag aatccgaacc cggggtcgag gtaaccgtct
     8941 ccttgctcgg caagtccaag gtgccggcgc tcgacggggc ggcggtcagc gtgctcttca
     9001 cgcagaacgt gcgcgaggac gtgctcagcg tgccgctgac cgcgctggtc gcgatcggct
     9061 ccgagcgctt cgccgtggtc gcgatcgaag gcaccgcgcg gcgccagatc gtcgtcaccc
     9121 ccggcctcgc cgccgacggc tacgtcgagg tcgagggcaa ggggctgcgc gagggcatgc
     9181 tggtggagac cggccagtga gcgccccggt gctcgagctg cgcgacgtgc gcaagtccta
     9241 ccccggcggg gtggaggcgc tgcgcggggt ctcgctcgcg atcggggcag gagagctgac
     9301 cgcgatcgtc ggcccctcgg gctcgggcaa gtcgaccctg ctgcac
//
LOCUS       DASRIG010000353         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3566170, whole genome shotgun sequence.
ACCESSION   DASRIG010000353 DASRIG010000000
VERSION     DASRIG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3566170"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFB52_09525"
     CDS             <1..1297
                     /locus_tag="VFB52_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HZO06616.1"
                     /translation="GSGDVTVGGPGGEQNANFIAGDGVPGSDVGISQTGGAGFEAVGN
                     RIGIGPVDEQGTAPPGNAIMIAGEAGAEPALVGENLIWAQGMGINSSGRGAEILDNVI
                     AGTSTGIRTLGGTGGDGNLIEGNAIRETSINGVVIENEANEVFGNEIEEVTFSAVFLK
                     GAAEGNRIGGDTTASENFIRDSEQTPIALVLPEASQNEVGRNRGKENGSSFIGLFKAN
                     GEEPKYPNGGIVPPTFANAYQSSASGTAQPGANVRVFGKETDQGGEIASFLGEATADG
                     TGAWTVNYSGSIPVGTNLAATQTNTLGGTSELAFAVASADPPPLPDPCIQTPLAPGCG
                     PAPCPPMGSECRPLVRPRVTITKAPKAKSTKTTAKFVFKSSVAGSKFKCKLDKKAFAN
                     CRSPKTYKKLKPGKHVFKVKATNSAGTSPVVTRKFTVLE"
     gene            1366..>2953
                     /locus_tag="VFB52_09530"
     CDS             1366..>2953
                     /locus_tag="VFB52_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZO06617.1"
                     /translation="MSLRPLVEIAAENERVHALCGRVREAAEGGDAVEVRASTMLRPL
                     LLAALLEDDRGLAGRPALLVAADDRSGRDLAADLRAYLAPRRVRFYPSRGTGYASSVS
                     PPPHLVGLRIDALDALSAEADAVVVASATALAEAVPDASLRPAGFAIAKGEEVELGAV
                     AEDLVAAGYERVEQVEERGQFAVRGGILDVYPATEERAVRIELFGDEVESMRWFSTFT
                     QRSLGESERVELAPAAELDAEHRELAELAAMAAEEDGGEAPNLAEALPLDEFRAPLDL
                     VPAEAAVLLAAAEEIEPALRDYWDDATTAMHANDAQHLYVDVAEPLAARAALSLTGAG
                     EEDEDAFRAARAESPARSVKEAESELEKLVRSGYRTVVAFDAFGEAERARYGLDRLDA
                     RMLEGGRLSPDPGLAFAEARLREGFVSPELKLAVYPFRRLVHRRKAEEPALAAGRGKL
                     AFSELRVGDFVVHEDHGVARFAGFETREIGGVTRDYLYLEYKGEDRVYVPTEQLAKLS
                     RYVGAGGSATLSALGGKRWQN"
BASE COUNT          479 a         1079 c         1011 g          384 t
ORIGIN      
        1 cggctccggc gacgtcacgg tcggcggccc gggcggtgaa cagaacgcga acttcatcgc
       61 cggcgacggt gtgcccggca gcgacgtcgg catctcccag accgggggcg ccggcttcga
      121 agcggtcggc aaccggatcg gtatcggccc ggtggatgaa cagggcaccg ccccgccagg
      181 caacgcgatc atgatcgccg gcgaagcagg tgccgaaccc gctctggtgg gcgaaaacct
      241 gatctgggcg caggggatgg ggatcaacag ctccggtcgc ggtgccgaaa tcctcgacaa
      301 cgtgatcgcc gggacgtcga ccgggatccg gaccctgggc ggtaccggcg gcgacggcaa
      361 cctgatcgaa ggcaacgcga tccgcgagac ctccatcaac ggggtcgtga tcgaaaacga
      421 ggcgaacgag gtcttcggca acgagatcga agaggtcacc ttctcggccg tcttcctcaa
      481 aggcgcggcc gaaggaaacc ggatcggcgg cgacaccacc gcctcggaaa acttcatccg
      541 cgacagcgaa cagaccccga tcgcgctggt cctcccggaa gcgagccaga acgaagtggg
      601 gcgcaaccgc ggcaaagaaa acggcagcag cttcatcggg ctgttcaagg ccaacgggga
      661 agaaccgaaa tatcccaacg gcgggatcgt gccgccgacc ttcgccaacg cctaccagtc
      721 gagcgcgagc ggcaccgccc agcccggcgc caacgtgcgc gtgttcggca aggaaaccga
      781 tcagggcggc gagatcgcct ccttcctcgg cgaagcgacg gcggacggaa ccggcgcctg
      841 gaccgtcaac tactccggtt ccatcccggt cgggacgaac ctcgcggcaa cccagacgaa
      901 caccctcggc ggcacctccg aactggcttt cgccgtcgcc tcggccgacc cgccgcccct
      961 gccggatccc tgcatccaga cgccgctggc cccaggctgt ggaccggccc catgtccgcc
     1021 gatgggcagt gaatgtcgcc cgttggttcg gccccgggtc acgatcacga aagcgccgaa
     1081 ggcgaaatcg acgaagacca cggcgaagtt cgtgttcaag tcctccgtcg ccggctcgaa
     1141 gttcaagtgc aagctcgaca agaaggcctt cgccaactgc cgctcgccga agacctacaa
     1201 gaagctgaag ccgggcaagc acgtcttcaa ggtgaaagcg accaactcgg ctggcaccag
     1261 cccggtcgtg acgcgcaagt tcacggtcct cgaatagccg ctcccccggc tccctagaat
     1321 cggtcgcaga gggccggatc ggacgatccg gtcgttctcc ctgccatgag cctccgcccc
     1381 ctggtcgaga tcgccgccga gaacgagcgc gtgcacgcgc tctgcggccg cgtgcgcgag
     1441 gccgcagagg gaggtgacgc ggtcgaggtg cgcgcctcga cgatgctgcg tccgctgctg
     1501 ctggcggcgc tgctcgagga cgaccgcggg ctcgccggcc ggccggcgct gctggtcgcc
     1561 gccgacgacc gctccggtcg cgacctcgcc gccgacctgc gcgcctacct ggccccgcgc
     1621 cgggtccgct tctacccctc gcgcgggacc gggtacgcct cctcggtctc gccgccgccg
     1681 cacctggtcg ggctgcggat cgacgcgctc gacgcgctct ccgccgaagc cgacgcggtt
     1741 gtcgtcgcca gtgccacggc gctcgccgag gcggttccgg acgcctcgct gcgccccgcc
     1801 gggttcgcga tcgccaaggg cgaggaagtc gagctcgggg cggtcgccga ggacctggtc
     1861 gcggctggct acgagcgggt cgagcaggtc gaggagcgcg gccagttcgc ggtccgcggc
     1921 ggcatcctcg acgtctaccc ggcgaccgag gaacgggccg tgcggatcga gctgttcggc
     1981 gacgaggtcg agtcgatgcg ctggttctcg actttcaccc agcgctcgct cggcgagtcc
     2041 gagcgggtcg agctggcccc ggcggccgag cttgacgccg agcaccgcga actcgccgag
     2101 ctggcggcga tggccgccga ggaggacggg ggagaggcgc cgaacctggc cgaggcgctg
     2161 ccgctcgatg agttccgcgc cccgctcgac ctcgtccccg ccgaggccgc ggtcctgctc
     2221 gccgccgccg aggagatcga gccggcgctg cgcgactact gggacgacgc gaccacggcg
     2281 atgcacgcca acgacgccca gcacctctac gtcgacgtcg ccgagccact cgccgcccgc
     2341 gccgcgctct cgctcaccgg ggcgggggag gaggacgagg acgccttccg cgccgcccgc
     2401 gccgagtcgc ccgcgcgcag cgtcaaagag gccgagtcgg agctggagaa actggtccgc
     2461 tccggctacc gcacggtcgt cgccttcgat gcctttggcg aggccgagcg cgcccgctac
     2521 gggctcgacc ggctcgacgc ccggatgctc gagggcggcc ggctctcgcc cgaccccggt
     2581 ctcgccttcg ccgaggcgcg cctgcgcgag ggcttcgtca gccccgagct gaaactcgcg
     2641 gtctatccct tccgccgcct cgtccaccgc cgtaaagccg aggagccggc gctcgccgcc
     2701 ggccgcggca aactcgcctt ctccgaactg cgggtcggcg acttcgtcgt ccacgaggac
     2761 cacggcgtcg cccgcttcgc cggcttcgag acgcgcgaga tcggcggcgt cacccgcgac
     2821 tacctctacc tcgaatacaa aggcgaggac cgcgtctacg tgccgaccga acagctggcg
     2881 aagctgagcc gctacgtcgg tgccggcggc tcggcgacgc tctcggcgct gggcggcaaa
     2941 cgctggcaga aca
//
LOCUS       DASRIG010000354         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_410610, whole genome shotgun sequence.
ACCESSION   DASRIG010000354 DASRIG010000000
VERSION     DASRIG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410610"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1927)
                     /locus_tag="VFB52_09535"
     CDS             complement(86..1927)
                     /locus_tag="VFB52_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06618.1"
                     /translation="MATDTAIELEQIYNPAEAEEAVGSRAFPNTRREGWLIALVFAVA
                     YGVLGYFTVVNGHVVQFDGLERLADAYMAWWNDPPKLAAIGLSVAPVGTLVFLAPALI
                     KPFATSLVALPMITAIAGGATLAMLNTLMARCGLGRLIRIGIVVLIGINPMFAFYAGN
                     GSPEALGLFLAASALLALISWQVTDETRYLIGAGMAIGVAVMVDYTYLAWAFSIMLAI
                     AFIGPGPRGGQTKLRSSLLLFLTPIFYSVLVWTILNAVILDSPFQWLEIGSVQVAPNL
                     DPAINQIPASLDGALSDLAEAVFGIAPLAFLALPLLVVSAFGRRDSLGLGLIAVLLSA
                     AGAIVIVALLEDRASYVTLSTVLPIMVASIASIAWLFRVENTWRFVLVLVAVVGMVLA
                     IPLSWNAMRNYDYQDQEQAFTRFLETRDSQEGTASLGGYRVGIDPELGMADYINNKLK
                     PENNSILTDSSVTYGVILMSGKPSLFVDRADYDEGEWQTIRDHPFGEVQYMLVANTGA
                     DLVRQRYPGLDLGAQPGLVPIFHTDRYVLVQLEPGVIPAASVATPDRLRNQPRLITPR
                     EPLAPPPVRPAGPGEAGLSPLPESGGGVSEEGSSPDGLSSEPQVEGE"
BASE COUNT          344 a          660 c          778 g          289 t
ORIGIN      
        1 ccgcaggcgg ccaggccggc ggtcagcacc agcgcgacca gggcgaggag gacgacgcgc
       61 agcgccttga tgttggcggc ggcgctcatt cgccttccac ctgcggttcg gagctgaggc
      121 cgtcggggct cgagccttcc tcgctcacgc caccgccgga ttcgggcagc ggcgagaggc
      181 ccgcttcgcc ggggccggcc gggcgcaccg gcggcggcgc cagcggctca cgcggggtga
      241 tcaggcgcgg ctggttgcgc aggcgatccg gggtcgccac cgaggcggcc gggatcacgc
      301 cgggctcgag ctggaccagc acgtagcggt cggtgtggaa gatcgggacc aggccgggct
      361 gcgcgccgag gtcgagcccc gggtagcgct ggcggaccag gtcggcgccg gtgttggcga
      421 cgagcatgta ctggacctcg ccgaacgggt ggtcgcggat cgtctgccat tcgccttcgt
      481 cgtagtcggc gcggtcgacg aacaggctcg gcttgccgga catcaggatc acgccgtagg
      541 tgaccgagct gtcggtgagg atcgagttgt tctccggctt cagtttgttg ttgatgtagt
      601 cggccatgcc gagctcggga tcgatcccga cccggtagcc gcccagcgac gccgtgccct
      661 cctggctgtc gcgggtctcg aggaagcggg taaaggcctg ctcctggtcc tggtagtcgt
      721 agttgcgcat cgcgttccag gagagcggga tcgccagcac catcccgacc acggcgacga
      781 gcacgagcac gaagcgccag gtgttctcga ctcggaacag ccaggcgatc gaggcaatcg
      841 aggcgaccat gatcggcagc accgtgctca gggtcacgta gctggcgcgg tcttcgagca
      901 gggcgacgat cacgatcgcg ccggccgctg acaagagcac ggcgatcagc ccgagcccga
      961 gcgagtcgcg gcggccgaag gcggagacga ccagcagcgg tagcgccagg aaggcgagcg
     1021 gcgcgatccc gaacacggct tcggcgaggt cgctgagggc gccgtcgagg ctggccggga
     1081 tctggttgat cgccgggtcg aggttgggcg ctacctggac cgagccgatt tccagccact
     1141 ggaaggggct gtcgaggatg accgcgttga ggatcgtcca gaccagcacc gagtagaaga
     1201 tcggggtcag gaagagcagc agcgaggagc gcagcttcgt ctggccgccg cgcgggccgg
     1261 ggccgatgaa ggcgatcgcg agcatgatcg agaaggccca ggcgaggtag gtgtagtcga
     1321 ccatcaccgc gaccccgatc gccatcccgg cgccgatcag gtagcgggtc tcgtcggtga
     1381 cctgccagga gatcagcgcc agcagcgccg aggcggcgag gaacagcccg agcgcttccg
     1441 gcgagccgtt gccggcgtag aaggcgaaca tcgggttgat cccgatcagc accacgatcc
     1501 cgatccggat caggcgcccg agcccgcagc gcgccatcag cgtgttcagc atcgccaggg
     1561 tggcgccgcc ggcgatcgcg gtgatcatcg gcagtgcgac cagcgaggtg gcgaacggct
     1621 tgatcagcgc cggggcgagg aagacgaggg tgccgaccgg ggcgacgctg agcccgatcg
     1681 cggccagctt cggcgggtcg ttccaccagg ccatgtaggc gtcggcaagc cgttccagcc
     1741 cgtcgaactg gacgacgtgc ccgttgacca cggtgaagta gccgagcacc ccgtaggcga
     1801 cggcgaagac gagggcgatc agccagccct cgcgccgcgt gttcgggaag gcccggctgc
     1861 cgacggcctc ctcggcctcc gccgggttgt agatctgctc cagctcgatc gcggtgtcgg
     1921 tggccatcag acgatcgtcc ccggcgaaga catgtcgagc ccgtgctcgg tcttctccca
     1981 gtagaagggg ttggtgaaca gctgcaggaa gcccttccag gcggccagcg acatcaggcc
     2041 ccagtagagc ggcagcgaga gcgcgtaggg g
//
LOCUS       DASRIG010000355         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_178532, whole genome shotgun sequence.
ACCESSION   DASRIG010000355 DASRIG010000000
VERSION     DASRIG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178532"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFB52_09540"
     CDS             <1..571
                     /locus_tag="VFB52_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZO06619.1"
                     /translation="ARRTALLLIATLALLGTTVATTSATTSAGGGLVASTSACPGQTK
                     LDAPVATQERAMLCMANYARAQLGLGALEATPPLEGSAKEKARDVLRCDSFSHYACGR
                     EFEYWIRASGYLSAPCWRAGENLAFGNGPLGSVRSIFRAWMRSPDHRENILGDYTQTG
                     IDLTTGTLEGFAGTRVWAQHFGTHCDAPA"
     gene            692..1444
                     /locus_tag="VFB52_09545"
     CDS             692..1444
                     /locus_tag="VFB52_09545"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HZO06620.1"
                     /translation="MAAILACGEGAFLTHLSAGALFGICEERPGRIEVGVPRPHGREP
                     SGIRLRRRPSLPSQDVGALRGIPVTSPVRTMIDLATEQGPKYLLRSINEADKLEVILA
                     DDLRVQLDAYVGVPGAKRLVALLDRDTFVLSDEELERLFLPLALEVGLSLPRTKIWLN
                     GFEVDFFWPELRLVVETDGLRYHRTPSAQARDLKRDQAHRGWLHPPALLALAGQVRPR
                     LRQGDPPRHRRQISGRLIRGAPRSGRRVRGRP"
     gene            complement(1383..1814)
                     /locus_tag="VFB52_09550"
     CDS             complement(1383..1814)
                     /locus_tag="VFB52_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06621.1"
                     /translation="MAEGERDNTSEWVLPEEARPATLAQLEERIDYAVAVARASEAAA
                     LEIGAAAIDAAEQARRAAELAERASGGGVAVAPAVDAPLTEEREAVVVEEFGGDGVVE
                     ELEAVTAEPEPAPEPQAEYDSFRAFLERADRISARLESIAR"
     gene            1932..2984
                     /locus_tag="VFB52_09555"
     CDS             1932..2984
                     /locus_tag="VFB52_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZO06622.1"
                     /translation="MRHPREFFLPQAIGAPTPLTEIPVKPSRMIHFLDFSNEKMVAKA
                     GDIAKQTDILLGNLEDAVPVDRKEAARAGLIKAAKETDFGGTSLWTRVNSLESPWVLD
                     DLTEIVTEIGDVLEVVMVPKVEGAWDIHYVDRLLAQLEAKAELERPILIHAILETAQG
                     VVNLEEIANASPRMQGMSFGPADLAASRRMKTTRVGGGHPGYRVMEDPADPENPEADR
                     VSAQQDLWHYSIARMVDACAAAGILPFYGPFGDIKDTAGCETQFRAAFLMGCVGAWSL
                     HPVQIGIAKKVFSPPPDEVKFAKKVIAAIPDGRGVHMIDGKMQDDATWKQCKVMVELA
                     EMLAEKDPELAASYEA"
     gene            3074..>3473
                     /locus_tag="VFB52_09560"
     CDS             3074..>3473
                     /locus_tag="VFB52_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06623.1"
                     /translation="MDRSSSQPRRSRRLAALLAVAAAFCLLPASAGAAVQARSSDSFV
                     DSIGVNTHLYYTDTPYHDEFGLVKQRLKELGIRHIRENLVPDREDQYEMLNELAGQGV
                     KSTLILGEPDDGQAGLNELVSVLKNDLQGSV"
BASE COUNT          555 a         1322 c         1092 g          504 t
ORIGIN      
        1 ggcccgccgc accgccctac ttctgatcgc caccctggcc ctactcggga cgaccgtcgc
       61 gaccacctcc gcgaccacct ccgccggcgg cggcttggtc gcatcaacca gcgcctgccc
      121 gggtcagacc aagctcgacg ccccggtcgc gacccaggag cgggcgatgc tctgcatggc
      181 caactacgcc cgcgcccagc tcggcctcgg cgccctcgaa gcaacccctc cgctggaagg
      241 gtcagcgaag gagaaggccc gcgacgtgct ccgctgcgac agcttcagcc actacgcctg
      301 cggccgcgaa ttcgagtact ggatccgcgc cagcggctac ctctccgccc cttgctggcg
      361 ggccggcgag aacctcgcct tcggcaacgg ccccctcggc agcgtgcgct cgatcttccg
      421 cgcctggatg cgctcccccg accaccgcga gaacatcctc ggcgactaca cccagaccgg
      481 catcgacctc acgaccggca ccctcgaagg cttcgccggc acccgcgtct gggcccagca
      541 cttcggcacc cactgcgacg cccccgcgta aaggggaaga ctctcccgcc ctctcgactt
      601 agtaggcgct atggcgccca agatgtcgag agcgcgaggt acccgggcct ggtgcgggcg
      661 cagcatggtg tggtcgcgcg agggcaggtg gatggcggcg atcctggcct gcggcgaggg
      721 cgcattcctt acccacctca gtgcgggcgc cctgttcggg atctgcgaag agcgcccagg
      781 gcggatcgaa gtcggcgtcc cgcgccccca cggacgagag ccctccggca tccgcctgcg
      841 ccgccgcccc tccctaccca gccaagacgt cggcgccctg agaggcatcc cggtcacctc
      901 gccggttcgc acgatgatcg acctcgccac cgagcagggg ccgaagtacc tgctgcgctc
      961 gatcaacgag gccgacaagc tggaggtgat cctggccgac gatttgcggg tgcaactcga
     1021 cgcctacgtt ggcgtcccgg gcgcgaagcg cctggtcgcc ctcctcgatc gggacacctt
     1081 cgtgttgagc gacgaggaac tggaacgcct tttcctccct ctggccctgg aggttggact
     1141 ctccctcccc cggaccaaga tctggctgaa cggcttcgag gtcgacttct tctggcccga
     1201 gcttcgcctg gtcgtcgaga ccgacgggct gcgctatcac cgcacccctt cagcccaggc
     1261 ccgagacctg aagcgcgatc aggcccaccg cggctggctt cacccgcctg cgcttctcgc
     1321 actggcaggt caagtacgac cccgcctacg tcaaggcgat cctccgcggc accgccgcca
     1381 gatcagcggg cgattgattc gaggcgcgcc gagatccggt cggcgcgttc gaggaaggcc
     1441 ctgaagctgt cgtattccgc ctgtggctcc ggcgctggct cgggctccgc cgtcaccgcc
     1501 tcgagctcct cgacgacccc gtccccgccg aactcctcaa ccaccacggc ctcacgctcc
     1561 tcggtcagcg gcgcgtcgac cgcgggcgcc acggcgacgc cgccgccgct cgcgcgttca
     1621 gccagctcag cggcccggcg ggcctgctcg gccgcgtcga tcgcggcggc gccgatctcc
     1681 agggccgcgg cttccgaggc gcgggccacg gcgaccgcgt agtcgatccg ctcctcgagc
     1741 tgggccaggg tcgccgggcg cgcctcctcg ggcagcaccc actcactcgt gttgtcccgc
     1801 tcgccctcgg ccatgacccc gcaatttacg gtcgccgcag gcgggaaacc catccggccc
     1861 gatcacaccg tgagtttccc cgtccattga ctagatttcg ccccatccag agaaacgaag
     1921 ctaggagtga cttgagacac ccgagagagt tcttcctgcc gcaggcgatc ggcgccccga
     1981 ccccgctgac cgagatcccg gtcaagccgt cgcggatgat ccacttcctc gacttctcga
     2041 acgagaagat ggtcgccaag gccggcgaca tcgccaagca gaccgacatc ctgctcggca
     2101 acctcgagga cgccgtgccg gtcgaccgca aggaggccgc ccgcgccggg ctgatcaaag
     2161 ccgcgaagga gaccgacttc ggcggaacct cgctgtggac ccgggtcaac tcgctcgaga
     2221 gcccttgggt gctcgacgac ctgaccgaga tcgtcaccga gatcggcgac gtgctcgagg
     2281 tcgtgatggt gccgaaggtc gagggcgcct gggacatcca ctacgtcgac cgcctgctcg
     2341 cccagctcga ggcgaaggcc gaactggagc gcccgatcct gatccacgcg atcctcgaga
     2401 ccgcgcaggg cgtggtcaac ctcgaggaga tcgccaacgc cagcccgcgc atgcagggga
     2461 tgagcttcgg ccccgccgac ctcgcagcct cgcggcggat gaagacgacc cgcgtcggcg
     2521 gtggccaccc cggctaccgg gtgatggaag acccggccga cccggagaac cccgaggccg
     2581 accgcgtgag cgcccagcag gacctctggc actactcgat cgcgcgcatg gtcgacgcct
     2641 gcgccgccgc cggcatcctg cccttctacg ggccgttcgg cgacatcaag gacaccgccg
     2701 gctgcgagac ccagttccgc gccgccttcc tgatgggctg cgtcggcgcc tggtcgctgc
     2761 acccggtcca gatcgggatc gcgaagaagg tgttcagccc gccgccggac gaggtcaagt
     2821 tcgcgaagaa agtgatcgcg gcgatccccg acgggcgcgg cgtccacatg atcgacggca
     2881 agatgcagga cgacgccacc tggaagcagt gcaaggtgat ggtcgaactg gccgagatgc
     2941 tggccgagaa ggacccggag ctggctgcct cctacgaggc ctgatgcacg cgccgctgcg
     3001 agcggcgtcg aacaagcgtc caaagagctt gctttagctc tggcggtgcc acggggggtc
     3061 cgagtgatcc cccgtggacc gctcttcttc ccagccccgc cgcagccgtc gcctcgcggc
     3121 cctgcttgcc gtcgccgccg ccttctgcct cctcccggcc agcgccggcg ccgccgtgca
     3181 ggcgcgctcg tcggactcct tcgtcgactc gatcggggtc aacacccacc tctactacac
     3241 cgacacgccc taccacgacg agttcgggct ggtcaagcag cggctgaagg aactcggcat
     3301 ccgccacatc cgcgagaacc tcgtccccga ccgcgaggac cagtacgaga tgctcaacga
     3361 gctcgccggc cagggcgtca agtcgaccct gatcctcggt gagcccgacg acggccaggc
     3421 cggcctcaac gagctcgtct ccgtcctcaa gaacgacctg cagggctcgg tcg
//
LOCUS       DASRIG010000356         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1731329, whole genome shotgun sequence.
ACCESSION   DASRIG010000356 DASRIG010000000
VERSION     DASRIG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731329"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFB52_09565"
     CDS             <1..950
                     /locus_tag="VFB52_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO06624.1"
                     /translation="VIIGNYGGDTEEVRRKVFWEPFESRTGVQVISADAGALAVPMIL
                     GEIPTKWDAIHGSISESWSAQLYGKKKLPTTPEIAWEDLVLPKKFQPYMWQSFFLAYV
                     PAAIKGNFDTEPASWADFFDTKKFPGKRAWPAGYFVGGQLEAALFADGVEADEMYPLD
                     LERAKAKISSIADDLVIYTEYAQAQSFLTSGTASMSYAPNGLWHELEEKGVEVSVIWD
                     ATPILDNNGMNILPEPPNPDAVEALAAFCNQPLLQAEFATMTNYGPPTEEAFEELTEE
                     QVEKLPNAPNRTNVFHDDPIYVAENYAEIEETTSSAIGE"
     gene            980..1906
                     /locus_tag="VFB52_09570"
     CDS             980..1906
                     /locus_tag="VFB52_09570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06625.1"
                     /translation="MSSTQSTPAPLAADRPPAVGGGRVLGKLRGAFDGSGAYWLLVPA
                     VLFLGLLLVVPLVYLLLLALETTPAGETGVGHVLSDADFLASLWRTFVLAAVVSVLTL
                     LLGTLYALALAVSPRWLAILMLVALFTIFWTSLLVRTYGWMLLYLPEGGLYTALHAVG
                     LRDTPLEIFQTNFASYPAMVHVMLPFVVFPVFAATRQIDPMQIRAARVLGARPPLILW
                     KVVLPALRAGMAAGAVLVFVMSLGFFVTPALLGSQTEPLVAQMIGQAFHAPGEAPVAA
                     AMSLALVVIVVVVYVVADRVFRVSETWGAEAK"
     gene            1903..2736
                     /locus_tag="VFB52_09575"
     CDS             1903..2736
                     /locus_tag="VFB52_09575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06626.1"
                     /translation="MNEELSKGLGLSRGAFVLLGVLFAIFMVLPLLVIVPTSWTAGQL
                     VEFPPEGFSFQWYEKALEDPRWTESFVVSLQVALFGAILATILGVAVALGMRRLVRGR
                     GTRVAQSLFILPLALPYVSYALGLYQMFTKLPAALNETLIPLILADAVITMPLVYVII
                     AGALAGVDPRLSRAASTMGARWPTILWRIELPLVRMAIIGAFIFSFAAIFDEATLAIF
                     LSPVTDVTLAQQLYRSAAESIQPTLSAVSTMITLLAILVLGIGTLFTRRRAGAPQGGE
                     A"
     gene            2733..>3338
                     /locus_tag="VFB52_09580"
     CDS             2733..>3338
                     /locus_tag="VFB52_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06627.1"
                     /translation="MSVELSLDDVRVELGGNEILCGVSIDIAPGEFLTLLGPSGSGKT
                     TTLNVIAGLVPNRGGHVRFDGESVDKRPAHDRDIGLVFQSYALFPHMTVGDNVSFPLR
                     TRKVPKPERGPIVERMLELVQLQGMGERKVSTLSGGQQQRIALARALSPSPSVLLLDE
                     PMAALDKQLRETMQIELKRIHEEVGVTTVAVTHDQTEAMTMS"
BASE COUNT          505 a         1209 c         1050 g          574 t
ORIGIN      
        1 aggtgatcat cggcaactac ggtggcgaca ccgaggaagt gcggcgcaaa gtcttctggg
       61 agccgttcga atcccggacc ggggtgcagg tcatctccgc tgacgccggc gccctcgcgg
      121 tgccgatgat cctcggcgaa atcccgacca aatgggacgc gatccacggc tcgatcagcg
      181 aatcctggtc ggcccagctg tacggcaaga aaaaactccc gaccaccccc gaaatcgcct
      241 gggaagacct cgtcctgccg aaaaaattcc agccgtacat gtggcagtcg ttcttcctcg
      301 cctacgtgcc ggcggcgatc aaaggcaatt tcgacaccga accggcctcc tgggcggact
      361 tcttcgacac gaagaaattc cccggcaagc gggcctggcc ggccggctac ttcgtcggcg
      421 gtcagctgga agcggcgctg ttcgccgacg gggtcgaagc ggacgaaatg tacccgctgg
      481 acctcgagcg ggcgaaggcg aaaatctcct cgatcgccga cgacctggtc atctacaccg
      541 agtacgccca ggcgcagtcg ttcctcacct cggggacggc ctcgatgtcc tacgcgccga
      601 acggcctctg gcacgagctc gaagaaaaag gcgtggaagt ctcggtgatc tgggacgcga
      661 cgccgatcct cgacaacaac gggatgaaca tcctgccgga accgccgaac ccggacgcgg
      721 tcgaggccct ggcggcgttc tgcaaccagc ccctcctgca ggccgaattc gcgaccatga
      781 ccaactacgg gccgccgacc gaagaggcgt tcgaagagct caccgaggaa caggtggaaa
      841 agctcccgaa cgcgccgaac cggaccaacg tcttccacga cgacccgatc tacgtcgccg
      901 aaaactacgc tgagatcgaa gaaacgacga gctcggcgat cggcgaatag cgccggcgga
      961 gcccgccgct ccctccccga tgtcatccac ccagtccacc ccggccccgc tcgccgccga
     1021 ccgccccccc gcggtcggcg gcggccgggt tctcggcaag ctgcgcggcg ccttcgacgg
     1081 ctccggcgcc tattggctgc tggtcccggc ggtgctgttc ctcggcctgc tgctggtcgt
     1141 gccgctggtc tacctgttgc tgctcgcgct ggaaacgacc ccggccggcg aaaccggcgt
     1201 cggccacgtg ctcagcgacg ccgacttcct ggcctcgctg tggcgcacct tcgtgctcgc
     1261 cgccgtggtc tccgttctga ccctgctcct cggcaccctc tacgccctgg ccctggcggt
     1321 ctccccgcgc tggttggcga tcctgatgct ggtcgccctg ttcacgatct tctggacctc
     1381 gctgctggtc cgcacctacg gctggatgct gctctacctg cccgagggtg ggctctacac
     1441 cgccctgcac gcggtcgggc tgcgcgacac gccgctggaa atcttccaga ccaacttcgc
     1501 ctcctacccg gcgatggtgc acgtgatgct gcccttcgtt gtcttcccgg tcttcgccgc
     1561 cacccgccag atcgacccga tgcagatccg cgccgcccgg gtgctggggg cgcggccacc
     1621 gctgatcctc tggaaggtgg tgttgccggc gctgcgggcg ggaatggcgg ccggggcggt
     1681 cctcgtcttc gtgatgtcgc tcggcttctt cgtcaccccg gccctgctcg gcagccagac
     1741 cgagccgttg gtcgcccaga tgatcggcca ggccttccac gcccccggcg aggccccggt
     1801 ggcggcggcg atgagcctcg ccctggtggt gatcgtggtc gtcgtctacg tcgtcgccga
     1861 ccgcgtcttc cgggtcagcg agacctgggg cgctgaggcg aaatgaacga ggagctgagc
     1921 aaaggcctcg gcctctcgcg cggcgccttc gtgctgctcg gcgtcctctt cgcgatcttc
     1981 atggtcctgc cgctgttggt gatcgtgccg acctcctgga ccgccgggca gctggtcgaa
     2041 ttcccccccg agggcttctc cttccagtgg tacgaaaagg cgctcgaaga tccgcgctgg
     2101 accgaatcct tcgtcgtctc gctgcaggtc gccctgttcg gcgcgatcct ggcgacgatc
     2161 ctcggggttg cggtcgcgct gggcatgcgg cggctggtcc gcggccgcgg cacccgcgtc
     2221 gcccagagcc tcttcatcct gccgctggcg ctgccctacg tctcctacgc gctcgggctc
     2281 taccagatgt tcaccaaact gccggcggcg ctgaacgaaa ccctgatccc gctgatcctc
     2341 gccgacgcgg tgatcacgat gccactggtc tacgtgatca tcgctggcgc cctggccggc
     2401 gtcgacccgc gcctcagccg cgccgcttcg acgatgggcg cgcgctggcc gacgatcctc
     2461 tggcggatcg agctgccgct ggtgcggatg gcgatcatcg gcgccttcat cttctccttc
     2521 gcggcgatct tcgacgaggc gaccctggcg atcttcctct cgccggtgac cgacgtcacc
     2581 ctcgcccagc agctctaccg ctctgccgcc gaatcgatcc agccgacgct ctcggccgtc
     2641 tcgacgatga tcaccctgct ggcgatcctc gtgctcggca tcggaacttt gttcacgcgc
     2701 cgccgtgccg gtgctcccca aggaggtgag gcatgagcgt cgagctcagc ctggatgacg
     2761 tccgcgtcga actcggcggc aacgagatcc tctgcggggt ctcgatcgac atcgcgccgg
     2821 gtgaattcct caccctgttg gggccctcgg gctccggcaa gacgacgacc ctgaacgtga
     2881 tcgccgggct cgttcccaac cgcggcggcc acgtccgctt cgacggcgag tcggtcgaca
     2941 agcggcccgc ccacgaccgc gacatcggcc tcgtcttcca gtcctacgcg ctgttcccac
     3001 acatgacagt cggcgacaac gtctccttcc cactgcgcac ccgcaaggtg ccgaagcccg
     3061 agcgcggacc gatcgtcgag cggatgctcg agctcgtgca gctgcagggc atgggggagc
     3121 gcaaggtctc gaccctctcc ggcggccagc agcagcggat cgcgctggcc cgggcgctct
     3181 cgccttcgcc cagcgtgctg ctcctcgacg agccgatggc ggcgctcgac aagcagctgc
     3241 gggagacgat gcagatcgag ctgaagcgga tccacgagga ggtcggcgtc accaccgttg
     3301 cggtcaccca cgaccagacc gaggcgatga cgatgtcg
//
LOCUS       DASRIG010000357         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2248128, whole genome shotgun sequence.
ACCESSION   DASRIG010000357 DASRIG010000000
VERSION     DASRIG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248128"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFB52_09585"
     CDS             <1..492
                     /locus_tag="VFB52_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZO06628.1"
                     /translation="GPRAGLGELRLPALQKGSSIMPGKVNPVIPEVVIQVAGQVIGND
                     AAITFAGSQGQFELNVRVPLIARNLLDSIKLLASASRLLDDKCVSGLEANEEMTERHA
                     EATLATATALNPHIGYDRAADIVKTAADSGRMLREVAIEKGVDEETLDKALDHRKMAR
                     PHD"
     gene            575..1228
                     /locus_tag="VFB52_09590"
     CDS             575..1228
                     /locus_tag="VFB52_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06629.1"
                     /translation="MYRTLKNHLNPASLLAVVAIFMAMGGVSYAAATKIGTKDIKKAA
                     VTTAKLKNDAVTGAKVKESSLGQVPKAAQATSADSATKAATATNAEQLGGIPAASFGS
                     GVVGAAITAPPVAAMAPSVATGAPIGVGEFQMPVPVPIKVKNLTVRAVGDFSRPFVLS
                     LRKPGGQALTCGGAVICTVPGPVSFQPGELMELAVTVPPGPAVFTGATYQVGYQIVP"
     gene            complement(1229..>3005)
                     /locus_tag="VFB52_09595"
     CDS             complement(1229..>3005)
                     /locus_tag="VFB52_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06630.1"
                     /translation="AALAAPHTTVERTIQDRDGDSRLEPAPGEDYSVIGADQGFRPPR
                     QGSILNLLQLSDFQMVDEESPARVEVIDPLSTPLNLPFGAAWRPWEALEPQISDAMIR
                     QVNQFTGASPVADGGGTHRAMDFTIDTGDSADSQQRNETLWVRTLLEGGSLNPNSGVD
                     KATYSNALCPKPLVPGAAEAAKYTGVQDYDDYIEGPYPEFYDPDSPQGPHSAWPKYPG
                     LMDKAQQAFTAAGLDVPSYVAFGNHDALVQGNAMANAAFEAVAMGCLKPIGPVPNPEN
                     AASFLAGLLDPTRLLASLLTGPQNTIVVPGDPQRQFVSKKQYKDIFKGGSQADGHGFD
                     LIDPAQESASKGAAGYYSWVPTPGMRFVALDTVAEAGTIFTPSGSTTSEGNIDDPQFK
                     WLRGQLEAATAADQLVVLFSHHAPESLNADAPDELALPCLAFRPALGHDLNPGCDLDP
                     RLSLPIHLEEDMVGLLHEFPNAIAWVAGHSHDNVVDAFPNPSGPGGFWSIRVAAEADW
                     PQQSRLLEVFDNHDGTLSIFGTILDHVGQSAAPPAGTPASSMDLNDLAAAGRTMSMND
                     IQSGAPAGEGSPADRNVELLIDDPR"
BASE COUNT          439 a         1021 c         1083 g          462 t
ORIGIN      
        1 gggccccgcg ccggtctcgg ggagctgcgc ctgccggcgc tccagaaggg ctcctcgatc
       61 atgccgggca aggtcaaccc ggtgatcccg gaggtcgtga tccaggtcgc cggccaggtg
      121 atcggcaacg acgccgcgat caccttcgcc ggctcccagg gccagttcga gctcaacgtg
      181 cgggtgccgc taatcgcccg caacctgctc gactcgatca agctgctggc ctcggcctcg
      241 cgcctgctcg acgacaaatg cgtctccggc ctcgaggcca acgaggagat gaccgagcgc
      301 cacgccgagg cgaccctcgc cacggcgacc gcgctcaacc cccacatcgg ctacgaccgc
      361 gccgccgaca tcgtcaaaac ggccgcggac agcggccgga tgctgcgcga ggtggcgatc
      421 gagaagggcg tcgacgagga gaccctcgac aaggccctcg accatcgcaa gatggcccgg
      481 ccgcacgact gagaattttt tggtcgccgc gtaattcggc gtcggtgacc tcgttggagg
      541 ggtgcaaacc agcactcgac caaggaggtc accgatgtac cgcacgctca agaaccacct
      601 gaatccggct tcgctgctcg ccgtcgtcgc gatcttcatg gcgatgggcg gcgtctccta
      661 cgccgccgcc accaagatcg gcaccaagga catcaagaag gccgccgtga ccacggcgaa
      721 actgaagaac gacgccgtca ccggtgccaa ggtcaaggag tcaagcctcg gtcaggtccc
      781 gaaggccgcc caggcgacca gcgcggacag cgccacgaaa gcggcgacgg cgaccaacgc
      841 cgagcagctc ggcggcatcc ctgcagcctc gttcggcagc ggcgtcgtcg gcgccgcgat
      901 caccgccccg ccggttgcgg cgatggcgcc gagcgtcgcc acgggcgctc cgatcggggt
      961 cggcgagttc cagatgccgg ttccggtccc gatcaaggtg aagaacctga cggtccgcgc
     1021 ggtcggcgac ttcagccgcc cgttcgtgct ctcgctgcgt aaaccgggtg gacaggccct
     1081 gacctgcggc ggtgccgtga tctgcaccgt tcccggtccg gtctccttcc agcccggcga
     1141 gctgatggag ctggcggtca ccgtgccgcc cggccccgcc gtgttcaccg gcgccaccta
     1201 ccaggtcggc taccagatcg tcccgtagtc agcgcgggtc atctatcaac agctcgacgt
     1261 tccggtcggc ggggcttccc tcgccggccg gagcgccgga ctggatgtcg ttcatcgaca
     1321 tcgtccggcc cgccgcggcg aggtcgttga ggtccatcga ggaggccggg gtcccggccg
     1381 gcggggccgc cgactggccg acgtggtcga ggatcgtgcc gaagatcgag agcgtgccgt
     1441 cgtggttgtc gaagacctcg agcaggcggc tctgctgcgg ccagtcggcc tccgcagcga
     1501 cccggatcga ccagaagcca cccggcccgg acgggttcgg gaaggcatcg acgacgttgt
     1561 cgtgcgagtg gccggcaacc caggcgatcg cgttcgggaa ttcgtgcagc agcccgacca
     1621 tgtcttcctc gaggtggatc ggcagcgaga ggcgcgggtc gaggtcgcag cccgggttga
     1681 ggtcgtgccc gagcgccggg cggaaggcga ggcagggcag cgccagctcg tccggcgcgt
     1741 cggcgttgag gctctccggc gcgtggtggc tgaagagcac gacgagctgg tcggcggcgg
     1801 tcgccgcttc cagctgcccg cgcagccatt tgaactgcgg gtcgtcgatg ttgccctcgg
     1861 aggtggtgct gccgctcggg gtgaagatcg tgcccgcctc ggcgaccgtg tcgagcgcga
     1921 cgaaccgcat ccccggcgtc ggcacccagg agtagtagcc ggcggcgcct ttcgaggccg
     1981 actcctgcgc cgggtcgatc aggtcgaagc cgtggccgtc agcctggctg ccgcccttga
     2041 agatgtcctt gtactgcttc ttggagacga actggcgttg cgggtcgccg ggcacgacga
     2101 tcgtgttctg cggcccggtc agcaacgagg cgagcaggcg cgtcgggtcg agcagcccgg
     2161 cgaggaagga ggccgcgttt tcagggttcg gaaccgggcc gatcggcttc aggcaaccca
     2221 tcgccaccgc ctcgaaggcg gcattggcca tcgcgttgcc ctggaccagg gcgtcgtggt
     2281 tgccgaaggc gacgtaggag ggcacgtcga gcccggcggc ggtgaacgcc tgctgcgcct
     2341 tgtccatcag gcccgggtat ttgggccatg ccgagtgcgg cccctgcggg ctgtcggggt
     2401 cgtagaactc cgggtagggg ccctcgatgt agtcgtcgta gtcctggacg ccggtgtatt
     2461 tggcggcctc ggcggcgccg ggcaccagcg gtttcgggca gagcgcgttc gagtaggtcg
     2521 ctttgtcgac gccgctgttc gggttcagcg agccgccctc gagcagcgtc cgcacccaga
     2581 gcgtctcgtt gcgctgctgg ctgtcggccg agtcgccggt gtcgatcgtg aagtccatcg
     2641 cccggtgggt gccgcccccg tcggcgaccg gactcgcgcc ggtgaactgg ttcacctgcc
     2701 ggatcatcgc gtcgctgatc tgcggctcca gcgcctccca gggccgccag gcggcgccga
     2761 aggggaggtt gagcggggtc gagagcgggt cgatcacctc gacccgggcc ggcgactcct
     2821 cgtcgaccat ctggaagtcg gagagctgga ggaggttgag gatcgacccc tggcgcgggg
     2881 ggcggaagcc ctggtcggcg ccgatcaccg agtagtcctc gcccggcgcc ggctcgagcc
     2941 ggctgtcgcc gtcgcggtcc tggatcgtcc gctcgaccgt ggtgtgcggc gcggccaggg
     3001 cggcc
//
LOCUS       DASRIG010000358         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1226780, whole genome shotgun sequence.
ACCESSION   DASRIG010000358 DASRIG010000000
VERSION     DASRIG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226780"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1514)
                     /gene="hemA"
                     /locus_tag="VFB52_09600"
     CDS             complement(183..1514)
                     /gene="hemA"
                     /locus_tag="VFB52_09600"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZO06631.1"
                     /translation="MSEILALGVSHKTAPLDLRERLALTEGNAAGALRELTAVDGIHE
                     AAAISTCNRTELYLIVSDPVEAESKALSVLTRQAEIRPTELLGHLYSLRAGEASRHLM
                     RVTAGLDSMILGEAEIQGQVKRAYELALVEGATGPILNRLFRGALAAGGRARDETGIS
                     QKGVSIPSVAVELASRALGDLTDRRVLMVGAGETAELVARALVARGVHTVFVANRHYD
                     RAIGLAQQFDGNAVRFEDLPQQLAEADIVVSATNSPHHIVERLELGEVMAARPGKALM
                     LIDIAVPRDVDPECREIPGVGVFDIDDVQRIAERNASGREAEAKRAELIIDAELDRFE
                     RWLASLEVVPTIAALRGRGDEIVNRVLAENENRWENLSEADRERVEAMAKAIASRLLH
                     EPTLRVRRSAGEEDAHFLLGALRELFGLDVETAPEGEARGNVTELRSKKQG"
     gene            complement(1514..1732)
                     /locus_tag="VFB52_09605"
     CDS             complement(1514..1732)
                     /locus_tag="VFB52_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06632.1"
                     /translation="MLPTILAAAPALPLDEAGKYVAGAYVVFVVLLVVYVAIMAAKLG
                     RIEKELREVAEYAERKKAEEKNPEGAGV"
     gene            complement(1732..2454)
                     /locus_tag="VFB52_09610"
     CDS             complement(1732..2454)
                     /locus_tag="VFB52_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HZO06633.1"
                     /translation="MYGKGLRPLSIATVAMLALSLSLVFFYAPLDADQGFIQKIFYLH
                     VPLAVVALIGFVVGGIFAIAHLRSGNSKWDAYSYVSIHMSVIFGVAVLITGSIWAKGS
                     WGHWWVWDEPTLVSFLIVFLLYCTYYPFRYAIEDRDRQARYASVFAITAGAFVPLNFM
                     AVRMAESLVHPRTFATAEGGLPAEMAWTVLACFVAMALLWTTLVKFELAAKSASGQVK
                     RLRRAIDAPGPANSSRIAPEVS"
     gene            complement(2489..3169)
                     /locus_tag="VFB52_09615"
     CDS             complement(2489..3169)
                     /locus_tag="VFB52_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZO06634.1"
                     /translation="MTPSRAAFAAILGKDLRAELRTLQSLPAMAIFAVTTFIIFRFGL
                     DRTQLSGDLASGVLWATLLFAAVLGINRLFVAEREEGGFDAMRLAPIDRSVLFAAKAS
                     ALIVYLLALELIAVPVFALFFLDSAEALGPLVLVLVLADIGLAATGTLISSMAVNSRA
                     RDLLVPLILLPLVVPLMIAATGATDPLLAVGGPGYERFGTWLMVLGLYDLIFALLGYA
                     VFDFLLED"
     gene            complement(3166..3855)
                     /locus_tag="VFB52_09620"
     CDS             complement(3166..3855)
                     /locus_tag="VFB52_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06635.1"
                     /translation="MASTQELPNPASGPAIVLAGLRRDFGERAALDGVGLELAVGETL
                     VVLGPNGAGKTTLLRILATLLRPSGGEVTVLGCALPGEAWKLRGQIGYLGHEPLLYRD
                     LSGRENLRFHARLQGLERDEAEARIEELLRNAGMDRRGDDRVVELSAGMRQRLAICRC
                     VLHRPALLLLDEPDSNLDAAGRELARGLIGPGAGQTRVIVTHDPDRFLPEADQVLRLG
                     IGERAAEAVAS"
     gene            3944..>4172
                     /locus_tag="VFB52_09625"
     CDS             3944..>4172
                     /locus_tag="VFB52_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06636.1"
                     /translation="MRVSKSVVLLAAACVAVLALGAAVVTAAEPGQTVTVKSEITIGA
                     VGYQGKVKAKNANCVEERTVVLKQVGNGVLSR"
BASE COUNT          663 a         1424 c         1519 g          566 t
ORIGIN      
        1 ctcggcctcg ccggccagca gcgcccgctc gaccccgcgc acgaagcgcg acttgtcgcc
       61 gggctcgccg tcggagctcg cctcgaccaa ctccgccccg cccagcagct cggcgacttc
      121 gcccgcctgg gtcagtgcga gggtgctgcc gcgagtggcg atccgcagcg ccaagcgccg
      181 cctcagccct gtttcttcga ccgcagctcg gtgacgttgc cgcgcgcctc gccctcgggc
      241 gcggtctcga cgtcgagacc gaagagctcg cgcagggcgc cgagcaggaa gtgggcgtcc
      301 tcctcgccgg ccgagcgtcg cacccgcagg gtcggctcgt gcaggagccg cgaggcgatc
      361 gccttcgcca tcgcctcgac ccgctcgcgg tcggcctcgc tgaggttctc ccagcggttc
      421 tcgttctcgg ccagcacgcg gttgacgatc tcgtcgccgc gcccgcgcag cgctgcgatc
      481 gtcggcacca cttccagcga ggcgagccag cgctcgaagc ggtcgagctc ggcgtcgatg
      541 atcagctccg cccgcttcgc ctcggcctcg cggcccgagg cgttgcgctc ggcgatccgc
      601 tgcacgtcgt cgatgtcgaa gacgccgacg cccggaatct cgcgacattc ggggtccacg
      661 tcgcgcggta cggcgatgtc gatcagcatc agcgccttgc cgggccgggc cgccatcacc
      721 tcgcccagct cgaggcgctc gacgatgtgg tggggagagt tggtcgccga gacgacgatg
      781 tcggcctcgg ccagctgctg cggcaggtcc tcgaagcgaa ccgcgttgcc gtcgaactgc
      841 tgggcgagcc cgatcgcgcg gtcgtagtgg cggttggcga cgaagacggt gtggacgccg
      901 cgggcgacca gcgcccgggc gaccagctcg gcggtctcgc cggcgccgac catcaagacg
      961 cggcggtcgg tgaggtcgcc gagcgcgcgg ctcgccagct cgaccgcaac cgaggggatc
     1021 gagacgccct tctggctgat cccggtctcg tcgcgggcgc ggccgccggc ggcgagggcg
     1081 ccgcggaaga ggcgattgag gatcgggccg gtggcgccct cgaccagggc cagctcgtag
     1141 gcgcgtttga cctgtccctg gatctcggcc tcgccgagga tcatcgagtc gagccccgcc
     1201 gtcacccgca tcaggtggcg cgaggcctcg ccggcgcgca gcgagtagag gtggccgagc
     1261 agctcggtcg ggcggatttc ggcctggcgg gtgagcaccg agagcgcctt cgactcggcc
     1321 tcgaccgggt cggagacgat caggtagagc tcggtccggt tgcaggtcga gatcgccgcc
     1381 gcctcgtgga tgccgtcgac cgcggtcagc tcgcgcaggg cgccggcggc attgccctcg
     1441 gtcagggcga ggcgctcgcg caggtcgagc ggcgcggtct tgtgcgagac cccgagggcg
     1501 aggatctcgg acatcagacc ccggccccct ccgggttctt ctcttcggct ttcttgcgct
     1561 ccgcgtactc cgcgacttcg cgcagttcct tctcgatccg gcccagtttc gcggccatga
     1621 tcgcgacgta gacgaccagc agcacgacga agacgacgta ggcgccggcg acgtacttgc
     1681 cggcctcatc gagcggcagc gcgggggccg cggcgagaat cgtgggcagc atcagctcac
     1741 ctccggggcg atccgcgagc tgttcgcggg gccgggggcg tcgatcgcac ggcgcaggcg
     1801 cttgacctgg ccggaggcgc tcttcgccgc cagctcgaac ttgacgaggg tggtccagag
     1861 cagcgccatc gcaacgaagc aggcgagcac ggtccaggcc atctcggccg gcagcccgcc
     1921 ttcggcggtg gcgaaggtgc gcgggtggac gaggctctcg gccatccgca cggccatgaa
     1981 gttgagcggc acgaaggcgc ccgcggtgat cgcgaagacc gaggcgtagc gggcctggcg
     2041 gtcgcggtcc tcgatcgcgt agcggaacgg gtagtaggtg cagtagagaa ggaagacgat
     2101 caggaagctg accagggttg gctcgtccca gacccaccag tgcccccagg agcccttcgc
     2161 ccagatcgag ccggtgatca gcacggcgac tccgaagatc accgacatgt ggatcgagac
     2221 gtacgaatac gcgtcccact tgctgttgcc gctgcgcagg tgggcgatcg cgaagatccc
     2281 gccgaccacg aacccgatca gcgcgacgac cgccagcggc acgtgcaggt agaagatctt
     2341 ctggatgaag ccttggtcgg cgtcgagcgg cgcgtagaag aagaccagcg aaagcgacag
     2401 ggccagcatc gccacggtgg cgatcgagag cggtctcagt cctttgccgt acatgcgtag
     2461 gaggtgcggg tgggaggccc ggtgcggatc agtcctcgag gagaaagtcg aacaccgcgt
     2521 aaccgagcaa tgcgaagatc agatcgtata ggccgagcac catcagccag gttccgaagc
     2581 gctcgtaacc gggaccgccc accgccagaa gggggtcggt ggcgccggtc gcggcgatca
     2641 tcagcggcac caccaggggc agcaggatca gcggcacgag caggtcgcgg gcgcgcgagt
     2701 tgaccgccat cgaggagatc agcgtgcccg tcgcggccag cccgatgtcg gccagcacga
     2761 ggaccagcac gagcggcccc agcgcctcgg ccgagtcgag gaagaagagc gcgaagaccg
     2821 ggaccgcgat cagctccagc gccagcaggt agacgatcag cgcactcgcc ttggcggcga
     2881 agaggacgct gcggtcgatc ggtgccagcc gcatcgcatc gaagccgccc tcctcgcgct
     2941 cggcgacgaa gaggcggttg atcccgagca ccgcggcgaa cagcagggtc gcccagagca
     3001 ccccggaggc gaggtcgccg gagagctggg tgcggtcgag gccgaagcgg aagatgatga
     3061 acgtggtgac ggcgaagatc gccatcgccg gcagcgactg cagggtgcgc agctcggccc
     3121 gcaggtcctt gccgaggatc gccgcgaagg cggcgcgcga gggggtcacg aggcgaccgc
     3181 ctccgcggcg cgctcgccga tccccaaacg caggacctgg tcggcctcgg gcaggaagcg
     3241 gtcggggtcg tgggtgacga tcacccgggt ctgcccggcg ccggggccaa tcagcccgcg
     3301 ggccagctcg cgccccgcgg cgtcgaggtt ggagtcgggc tcgtcgagca acagcagcgc
     3361 cgggcggtgc aggacgcagc ggcagatagc gagccgctgg cgcatcccgg ccgagagctc
     3421 gaccacgcgg tcatcgccgc gccggtccat cccggcgttc ctcagcagct cctcgatccg
     3481 ggcctcggcc tcgtcccgct ccagtccctg cagcctcgcg tggaagcgca ggttctcgcg
     3541 cccggagagg tcgcggtaga gcagtggttc gtggccgagg taaccgatct gaccgcgcag
     3601 cttccaggcc tcacccggca gcgcgcagcc gagcaccgtg acctcgccgc cgctcgggcg
     3661 cagcagcgtc gccaggatcc gcagcagcgt cgtcttgccg gcgccgttgg gcccgagcac
     3721 gaccaaggtc tcgccgaccg ccagctccag cccgaccccg tcgagcgcgg cgcgttcgcc
     3781 gaagtcgcgg cgcagccccg cgagcacgat cgccggtccg ctcgccgggt tgggtaattc
     3841 ctgcgtagaa gccatgtaag tcgattgtga aggctggccg atgatctagg ctgccgccgc
     3901 gactcgcggc aagaggcgca gcaaactcga gaaggagaac tggatgaggg tgagcaaatc
     3961 ggtggtcctg ctggcggcgg cctgtgtcgc ggtgctggcg cttggcgccg cggtcgtgac
     4021 ggcggccgaa ccgggtcaga cggtaacggt gaagtccgaa atcacgatcg gcgccgtcgg
     4081 ctaccagggc aaggtcaagg cgaaaaacgc caactgcgtc gaggagcgga ccgtggtcct
     4141 caagcaggtg ggcaacggcg tgctcagccg cg
//
LOCUS       DASRIG010000359         7292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3568468, whole genome shotgun sequence.
ACCESSION   DASRIG010000359 DASRIG010000000
VERSION     DASRIG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568468"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1095
                     /locus_tag="VFB52_09630"
     CDS             184..1095
                     /locus_tag="VFB52_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZO06637.1"
                     /translation="MATLRQMEYWLAAVEEGSFRRGAKRMHVSQPSLSQQIRALEAEL
                     GGELLERLPSGIRLTPAGKEFLPHARSTVAAAGRAVRSARSALELKSGELEIATVRSI
                     AAGILPDLIHSWRGRFPGASIRLQEFTHRKLAEDAVADGLADMGIGPPPQAWSGPVSR
                     LGWEELMVILPEDDSEADAESVRLDSLAEREWVMFHPDNGMSDLIVTACAVAVRQPFS
                     PRPAVYTSQVETAARLAAAGVGPTLVPASVVPAGLRGRVLPCRPAVGRELSVFARAEP
                     PALATAFLDLMRETEWSQPPAGAVIIA"
     gene            complement(1085..2110)
                     /locus_tag="VFB52_09635"
     CDS             complement(1085..2110)
                     /locus_tag="VFB52_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZO06638.1"
                     /translation="MRKITLRDVADRAQVHVSTASRALDESQSERLSPATIKRVRSAA
                     SELGYARDLVASGLKRGATKTVGVVVADLDNPHNAPVIRGIARELESEDFVPLVAETA
                     EDRGRFERLLSHLTQRRVDAIVTSAAHLDGAEILERILGPEASVVLAIRGLPGSDYNT
                     VVHDDRTGGSLAAQHLVSLGHEALAQLAGPTDIDTFVRRAEGFSRAVAAAGRREIGVG
                     GRTDAPSFDEGRRLMEVVLNGPELPTAVFAPTDVMAVGALDAIAGRGLRCPEDVSVVG
                     YNDIPISRYISPALTTIELPSEQLGQAAARMALDLIANPEDRGRLVELPARLIARRST
                     GAPTLRR"
     gene            complement(2150..3349)
                     /locus_tag="VFB52_09640"
     CDS             complement(2150..3349)
                     /locus_tag="VFB52_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZO06639.1"
                     /translation="MSTERSELGAFAHGAFLTDYEARADMGELRAERLEAARAALAAS
                     ELDALLLWKDENVRYVSGLRAQLIQGKTALLNGCLLTEDELVLFVSGGEIDRVNAVMP
                     WIDEVHAVPIMEARGLVSGAVEHVVGPALKRAGLATARVGLDELAFVQVQELGAQLPG
                     LLLDDGDAVMQEASAIAEAVTETAIATVAPGVREYDVVAEAMRTLYKLGGEMAHLATP
                     FVASGEHMAPPNRFASDKLIREGDLVFIDIGAMWSGYFSDMGRTVICGEPSRRQQEVF
                     TAVHASLRAATEAMRPGNTNEDVAAAVRAAGARYGLDDSFLSLFIGHGVGIGANEPPY
                     VGESLPGDETVELEEGMTMAIEPLIWVPGVQGGAGVRLEDTIVVREGGGLALTRTGFD
                     QRLLLDD"
     gene            complement(3373..4341)
                     /locus_tag="VFB52_09645"
     CDS             complement(3373..4341)
                     /locus_tag="VFB52_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06640.1"
                     /translation="MSAIAARLGRIERTGFGAAGAAYLVLVSLFVIAAIASPDFLDKQ
                     NLLNIGRQAAPLAIVALGQTIVILCGGIDVSVGAVMSLTTVVVSQEMGGDPSMVLPAV
                     MIVLALGAVIGLANGVLISRVGTDPFVTTLAALLIVQGAALVYTQGTPSNGLTPGFRQ
                     ISEGELLGIPASIFFAAAVFALVWFLLGRTIWGKSIYAIGGNLRATYLSGRRTALIQT
                     SAYVACSLLAVVAGLLLAARIGTAEVKAGSGFELEAIAAVLIGGTAFGGGRGRVAGTI
                     AGVLILVILFNLVNLLSLPSEIQLIVRGLVIVLGVAVYSRRPASGS"
     gene            complement(4338..5402)
                     /locus_tag="VFB52_09650"
     CDS             complement(4338..5402)
                     /locus_tag="VFB52_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06641.1"
                     /translation="MSAPRSVSKAVRLHRASNRWLGPLWVWAVLLVLVIVAIYTVPEF
                     ASIDNAKNILRQAVPLAIVTIGQTAVIVLGGIDLSVAAVLALGNTLSMGLMDGGEGSM
                     LLAILLPLLAGLLVGTINGLVVARTAAPAFIVTLGAASVVQGIVFWYTSSGPYGSPSL
                     SFGELGFKEYGPLPALVVFFIPLLVAGLIVQNRTRTGRHIYAVGDDDRVAERAGVHVA
                     RVKVGVYALSGVLAALAGIAVATRTGSGDPLAGVGFDWDSVAAAVIGGAVLVGGRGTI
                     GGAMAGVLIIAVIDNAMTLKDVSAFWQSTVKGVIVLVAVIIAALAAKDVLRTYLRRGP
                     MSNFKHPPPAGSASKGGAGA"
     gene            complement(5399..6919)
                     /locus_tag="VFB52_09655"
     CDS             complement(5399..6919)
                     /locus_tag="VFB52_09655"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO06642.1"
                     /translation="MSRLTVEEVSKRFGVVQALDRVSFDVERGEIHALVGENGAGKST
                     LTRIIGAAEAADSGRIEFEGRPLDLSSPVAALSAGISIAYQHLNLSTELTVAGNLFLG
                     REPRRLLGGVDDRGMNAAAAVWLERLGAGFPPDELVEGLSPSDRQLAEIARALSHEPT
                     LLIMDEPTSALAAHSAERMFSVLRELREEGMSVIYITHELAHVFELCDRVTVLKDGRS
                     TLTRPLAETDRDEVIRAMVGRTVSALFPPRTTPIDDAAVAALEVRELTLPEYFEDVSF
                     SIQPGEIVGLGGLIGSGRSRLAKAIFGVPPERSYGPLDGEILIHGEPVRPSGPRAAIE
                     HGIGFVPEDRKAAGLVLTASVADNISLPQLERISTAGVIRRGVEEDVAGGQVEALRIK
                     TPSLETEVGDLSGGNQQKVVLGKWLARGSSVLILDEPTPGVDIGAKAEIYALIRELAE
                     QKTAILLISSDLPELIGLSDRILVMSRGRVVAQLAQGDASEESVMRAAFDGLEAPA"
     gene            complement(6963..>7292)
                     /locus_tag="VFB52_09660"
     CDS             complement(6963..>7292)
                     /locus_tag="VFB52_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06643.1"
                     /translation="EGAFEAVKDAGRVDEVKVWTGDTVQGWMRTVDKEELPGVVVDRP
                     TIVGAESMALCVAILEGKPVPKLWSTENVVIDQTEMSEYIAKDTPGSSEWWDWWNLPE
                     KWLPKSS"
BASE COUNT         1056 a         2644 c         2541 g         1051 t
ORIGIN      
        1 ggcctcggcg agcggcgcga tgcggtcgcc gccgaccgcg gcgagggggg tgtccgaagc
       61 ggcgaggtga gcgaggcgca tcggggaatt ttccctctac agagcggttc tgtccaagtg
      121 gattctccac cgcaagtgat agccaaaacc tatcgctatg gcgttttctg cttgtctgtg
      181 ccgatggcca ctctgcgcca gatggaatat tggctcgccg cggtcgagga gggctcgttc
      241 cggcgcggcg ccaagcgcat gcacgtctcc cagccctccc tctcccagca gatccgcgcg
      301 ctcgaggcgg agctcggcgg cgagctgctg gagcgcctgc cgagcgggat ccgcctcacc
      361 cccgccggca aggagttcct cccccacgcg cgcagcaccg tcgccgccgc cggccgtgcc
      421 gtgcgctccg cccgctcggc gctggagctg aagagcggcg agctggagat cgccacggtg
      481 cgctcgatcg ccgccggcat cctccccgat ctgatccaca gctggcgtgg gcgctttccc
      541 ggcgcctcga tccggctgca ggagttcacg caccgcaagc tcgccgagga cgcggtcgcc
      601 gacgggctcg ccgacatggg gatcgggccg cccccgcagg cctggtcggg gccggtctcg
      661 cggctcggct gggaggagct gatggtgatc cttcccgagg acgacagcga ggccgacgcc
      721 gagtccgttc ggctcgactc gctggccgag cgcgagtggg tgatgttcca tcccgacaac
      781 ggcatgtccg acctgatcgt caccgcctgc gcggtcgccg tccgccagcc cttcagcccc
      841 cgccccgccg tctacacctc gcaggtcgag accgcggcgc ggctggccgc cgccggggtc
      901 ggcccgaccc tggtcccggc cagcgtcgtc ccggccggcc tgcgcggccg ggtgctcccc
      961 tgccggcccg cggtcggccg cgagctgagc gtcttcgcgc gggccgagcc gcccgccctc
     1021 gccaccgcct tcctcgacct gatgcgggag accgagtggt cccagccgcc ggccggcgcc
     1081 gtgatcatcg cctgagcgtc ggcgctccgg tcgagcgccg cgcgatcagc cgcgccggca
     1141 gctcgaccag gcggccgcgg tcctccgggt tggcgatcag gtcgagcgcc atccgcgccg
     1201 ccgcctgccc gagctgctcg gagggcagct cgatcgtggt cagcgccggc gagatgtagc
     1261 ggctgatcgg aatgtcgttg tagccgacca cggagacgtc ctcggggcag cgcaggccgc
     1321 ggccggcgat cgcgtcgagg gcgccgaccg ccatcacgtc ggtcggcgcg aagaccgcgg
     1381 tcggcagctc cggaccgttc agcacgacct ccatcagccg ccgtccttcg tcgaaggagg
     1441 gagcgtcggt gcggccccct accccgatct cgcgccgccc ggcggcggcc accgcgcggc
     1501 tgaacccctc cgcccggcgc acgaaggtgt cgatgtcggt cggaccggcc agctgcgcca
     1561 gcgcctcgtg cccgagcgag acgaggtgct gggcggccag cgagccaccg gtgcggtcgt
     1621 cgtggaccac cgtgttgtag tcgctgcccg gcagccccct gatcgccagc accacagacg
     1681 cctcgggacc gaggatgcgc tcgaggatct ccgccccgtc gaggtgggcg gcggaggtga
     1741 cgatcgcgtc gacgcggcgc tgggtcaggt gcgagagcag gcgttcgaag cgcccgcgat
     1801 cctcggcggt ctcggcgacc agcggcacga aatcttcgct ttccagctcg cgggcgatgc
     1861 cgcggatgac cggcgcgttg tgcgggttgt cgaggtcggc gacgaccacg ccgacggtct
     1921 tcgtcgcccc ccgcttcagc cccgaggcga ccaggtcgcg cgcgtagcca agctcgctcg
     1981 cggcgctgcg cacgcgcttg atcgtcgccg ggctgagccg ctcgctctgg ctctcgtcga
     2041 gtgcccgtga ggcggtcgag acgtgcacct gggcgcggtc ggcgacgtcg cggagggtga
     2101 tcttcctcat gcgcaatcca taacagacgc cccacgggga accgtgacct cagtcgtcga
     2161 gcagcaggcg ctggtcgaac ccggtccggg tcagggccag cccacccccc tcgcggacca
     2221 cgatcgtgtc ctccagcctc accccggcgc cgccctgcac gccggggacc cagatcaacg
     2281 gctcgatcgc catcgtcatc ccctcctcca gctcgaccgt ctcgtccccc ggcagcgact
     2341 cgccgacgta gggcggctcg ttggcgccga tcccgactcc gtggccgatg aacagcgaga
     2401 ggaaggagtc gtcgaggccg tagcgggccc cggcggcgcg cacggcggcg gcgacgtcct
     2461 cgttggtgtt gccgggccgc atcgcctccg tcgccgcccg cagcgaggcg tgcacggcgg
     2521 tgaagacctc ctgctggcgg cggctcggct cgccgcagat caccgtccgc cccatgtcgg
     2581 agaagtagcc gctccacatc gcgccgatgt cgatgaagac gaggtcgccc tcgcggatca
     2641 gcttgtcgga ggcgaagcgg ttgggcggcg ccatgtgctc gcccgaggcg acgaagggcg
     2701 tcgccaggtg ggccatctcg ccgcccagct tgtagagcgt gcgcatcgcc tcggcaacga
     2761 cgtcgtactc gcgcaccccc ggggcgacgg tggcgatcgc ggtctcggtg accgcctcgg
     2821 cgatcgcgct cgcctcctgc atcaccgcgt cgccgtcgtc gagcagcagg ccgggcagct
     2881 gtgcgcccag ctcctgcacc tggacgaagg cgagctcatc gaggccgacc cgggcggtgg
     2941 cgaggccggc ccgcttgagc gccggcccga ccacgtgctc gaccgcgccg gagaccaggc
     3001 cgcgcgcctc catgatcggc accgcgtgga cctcgtcgat ccacggcatc accgcgttga
     3061 cgcggtcgat ctcgccgccg gagacgaaca gcacgagctc gtcctcggtc agcaggcagc
     3121 cgttgaggag cgcggtcttg ccctggatca gctgcgcgcg cagaccgctc acgtagcgca
     3181 cgttctcgtc cttccagagc agcagagcgt cgagctcgga ggcggcgagc gcggcccggg
     3241 ccgcctccag ccgctcggct cgcagctccc ccatgtcggc gcgggcctcg tagtcggtga
     3301 ggaaggcgcc gtgtgcgaac gcgcccagct cggatctctc ggtggacatc ttggttgctc
     3361 ctttccctgg cttcacgatc cgctcgccgg acgcctgctg tacaccgcta cgccgagcac
     3421 gatcacgagg ccccgcacga tcagctggat ctcgctcggc agcgaaagca ggttgacgag
     3481 gttgaagagg atcaccagga tcaggacgcc ggcgatcgtg cccgcgaccc ggccgcggcc
     3541 gccgccgaag gccgtgccgc cgatcagcac ggcggcgatc gcttccagtt cgaagccgct
     3601 gccggctttc acttcggcgg tcccgatccg cgccgccagc agcaggccgg cgacgaccgc
     3661 gagcaggctg caggcgacgt aggcggaggt ctggatcaag gcggtgcggc gtccggagag
     3721 gtaggtcgca cgcaggttgc cgccgattgc gtagatcgac ttgccccaga tcgtgcggcc
     3781 gagcaggaac cagaccagcg cgaagacggc ggcggcaaag aagatcgagg cggggatgcc
     3841 gagcagctca ccttcggaga tctggcggaa gccgggggtc agcccgttgc tcggcgtgcc
     3901 ctgggtgtag accagcgccg cgccctggac gatcaacagc gccgccagcg tggtcacgaa
     3961 ggggtcggtg ccgacccgcg agatcagcac cccgttggcc aggccgatca ccgcgccgag
     4021 cgccagcacg atcatcaccg ccggcagcac catcgagggg tcgccgccca tctcctggct
     4081 gaccaccacc gtggtcagcg acatcaccgc gccgaccgag acgtcgatgc cgccgcagag
     4141 gatcacgatc gtctggccga gtgcgacgat cgccagcggc gccgcctggc ggccgatgtt
     4201 gagcaggttc tgcttgtcga ggaagtcggg tgaggcgatc gcggcgatca cgaaaaggga
     4261 gacgaggacc aggtaggcgg cccccgcggc gccgaatccg gttcgctcga tccgccccag
     4321 ccgggcggcg atcgcgctca tgctcccgcc cctcccttgc tcgcggagcc ggccggcggc
     4381 gggtgcttga agttcgacat cgggccacgc cgcaggtagg tgcgcagcac gtccttggcg
     4441 gcgagcgcgg cgatgatcac cgcgaccagg acgatcacgc ctttcaccgt cgactgccag
     4501 aaggcagaga cgtccttcag cgtcatcgcg ttgtcgatca cggcgatgat cagcacgccg
     4561 gccatcgcac cgccgatcgt gccgcgaccg ccgaccagca cggcgccgcc gatcaccgcg
     4621 gcggcgaccg aatcccagtc gaagccgacc ccggccagcg ggtcgccgga acccgtgcgg
     4681 gtggcgaccg cgatcccggc cagcgccgcc agcaccccgg agagcgcgta gacgccgacc
     4741 ttgacccggg cgacgtggac gccggcccgc tcggccaccc ggtcgtcgtc gccgaccgcg
     4801 tagatgtggc ggccggtgcg ggtgcggttc tggacgatca gcccggcgac cagcagcggt
     4861 atgaagaaca cgaccagcgc cggcagcggc ccgtattcct tgaacccgag ttcgccgaag
     4921 gagagcgaag gtgagccgta ggggccggag ctcgtgtacc agaagacgat cccctggacg
     4981 accgaggcgg cgccgagggt gacgatgaaa gcgggcgccg cggtgcgcgc cacgaccagc
     5041 ccgttgatcg tcccgaccag gaggccggcg agcagcggca gcaggatcgc cagcagcatc
     5101 gagccctcgc cgccgtccat cagccccatc gagagcgtgt tgccgagggc cagcacggcg
     5161 gcgaccgaga ggtcgatgcc gccgagcacg atcaccgcgg tttggccgat cgtgacgatc
     5221 gccagcggca ccgcctggcg caggatgttc ttcgcgttgt cgatcgaggc gaactccggc
     5281 acggtgtaga tcgcgacgat caccagcacc aggagcaccg cccagaccca gagcggtccg
     5341 agccagcggt tgctggcacg gtgcaagcgc accgccttgc tgaccgagcg gggggcgctc
     5401 acgcgggggc ctcgagaccg tcgaaggcgg cgcgcatcac gctctcctcg ctggcgtcac
     5461 cttgggcaag ctgggcgacg accctgccgc gactcatcac caggatgcgg tcgcttaacc
     5521 cgatcagctc gggcaaatcg gaggagatga gcaggatcgc cgtcttttgc tcggccagct
     5581 cgcggatcag cgcgtagatc tcggccttgg cgccgatgtc gaccccgggg gtcggctcgt
     5641 cgaggatcaa aaccgagctg ccccgggcca gccacttgcc gagcacgacc ttctgctggt
     5701 tgccgccgct gaggtcgccg acctcggtct cgagcgaggg cgtcttgatc cgcagcgcct
     5761 cgacctggcc gccggccacg tcctcctcga ccccgcggcg gatcacgccg gcggtcgaga
     5821 tccgctccag ctgcgggagc gagatgttgt cggcgaccga ggcggtgagc acgaggccgg
     5881 cggccttgcg gtcctcgggc acgaacccga tcccgtgctc gatcgcggcc cgcggcccgc
     5941 tcgggcgcac cggctcgccg tggatcagga tctcgccgtc gagcggcccg tagctgcgct
     6001 ccggcggcac cccgaagatc gccttggcaa gccgcgagcg gccggatccg atcaggccgc
     6061 cgaggccgac gatctcgccc ggctggatgc tgaacgagac gtcctcgaag tattcgggca
     6121 gggtcagctc gcgcacctcg agcgccgcca ccgccgcgtc gtcgatcggc gtcgtgcgcg
     6181 gcgggaagag cgccgagacg gtgcggccga ccatcgcccg gatcacttcg tcgcggtcgg
     6241 tctcggcgag cggcctggtc agcgtgctgc gaccgtcttt gagcaccgtc acccggtcgc
     6301 agagctcgaa cacgtgggcg agctcgtggg tgatgtagat gaccgacatc ccctcctcgc
     6361 gcagttcccg cagaaccgag aacatccgct cggcgctgtg cgcggccagg gccgaggtcg
     6421 gctcgtccat gatcagcagc gtcggctcgt ggctgagcgc ccgggcgatc tcggccagct
     6481 gccggtcgga ggggctgagg ccctcgacca gttcgtcggg ggggaagccg gcgccgagcc
     6541 gctccagcca gaccgccgcg gccgcgttca tccccctgtc gtcgaccccg ccgagcagcc
     6601 gccggggctc ccggccgagg aacagattgc ccgccaccgt gagctccgtg ctcaggttca
     6661 agtgctggta ggcaatgctg atcccggccg agagcgcagc gacgggcgag gagagatcga
     6721 gcggtcttcc ttcgaactcg atccgtcccg agtcagccgc ttcggcggcg ccgatgatcc
     6781 gcgtcagggt cgacttgccg gccccgttct cgcccaccag ggcgtggatc tcgccgcgct
     6841 cgacgtcgaa cgagacccgg tccagggcct ggacgacgcc gaagcgcttt gacacctcct
     6901 cgactgtgag tcgggacatt ctcgagctcc tcgatgactg cgtcagctcc ggtcagctcc
     6961 gctcaggagc ttttcggcag ccacttttcc ggcaggttcc accagtccca ccattcgctg
     7021 gaaccggggg tgtctttggc gatgtactcg ctcatttcgg tctggtcgat gacgacgttc
     7081 tcggtcgacc acagcttcgg caccggtttg ccttcgagga tcgcgacgca gagcgccatc
     7141 gactcggcgc cgacgatcgt cggccggtca acgaccacgc cggggagttc ttctttgtcg
     7201 acggtgcgca tccagccctg caccgtgtcg ccggtccaga ctttgacttc gtcgacccgg
     7261 ccggcgtctt tgaccgcctc gaaggcgcct tc
//
LOCUS       DASRIG010000360         6976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_179273, whole genome shotgun sequence.
ACCESSION   DASRIG010000360 DASRIG010000000
VERSION     DASRIG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179273"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1313)
                     /locus_tag="VFB52_09665"
     CDS             complement(48..1313)
                     /locus_tag="VFB52_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZO06644.1"
                     /translation="MAATTATPAVDLTDVIQPGDGVIWPQGTGEPRTLTEAFVRDLPA
                     YPGIEVFFGGTFSTTFDEADLSQTTVTVLGGAGANTRLAPPGELAVIPCHVSTLPGLI
                     ERGLIRADVVLIQVSPAGEDGSHSLGVIADYVHEAIAAARVVVAEVNERMPRTGGDTR
                     VEPGQIDRFVHSDRPLVSVTAPPPGPVEEAIAAHVAELVPDGATLEVGFGTVPNAILA
                     CLRGHRDLGLHTGAVGDEMVELIASGAMTNARKPIDTGVSVAGALFGTERLYDFADEN
                     PELEVRALAHTHNPRTIAQLGTFVAINGALEVDLTGQVNAETLRGRHVGAVGGQVDFV
                     RAAMASPGGRSIIALPATAAKGTASRIVARLADGVVTTARSDADVVVTEHGAAHLCGC
                     FLPERARRLIAIADPAFRDELTETARNLC"
     gene            complement(1313..2791)
                     /locus_tag="VFB52_09670"
     CDS             complement(1313..2791)
                     /locus_tag="VFB52_09670"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO06645.1"
                     /translation="MATEPIAAPVVERSWGHLIGGEWTVPGPALAAVDPANGAQIAEI
                     ADGDAADVEAAVAAAAAAFPRWRRSSPAARAALLHELADRVQAHAPTLAALEAWDAGK
                     PVRQATTWDLPLCAEVLRYYAAVARTSGGEVSEPNAGTLQIVRRRPAGPVGQIIPWNA
                     TVLMVAMKVAPALAAGCTVVLKPSELASLPALELLRVWADALPPGTVNLVTGGPEVGR
                     AIVSAPELRRISFTGSPAVGRQVMAAAAERLTPVMLELGGKSPSIVFPDADLDAAVEG
                     TLVGIIPNQGQMCAAGTRLLLHERIADEFLAKLGERIAGLRIGPPLDPTSDLGPLVSA
                     GQREKVLARIEEATAAGAREVFRLDPPDGLPEGGNWCPVVGFEATPEMKVAREEVFGP
                     VLSVLRWEDEKALPELANDVATGLAAAVWCADAGRALALLDRVDAGVAWVNTYGFLPP
                     GAPFGGVKDSGFGRENGAAALDEYRDLQTVLVRHSGPGVQLY"
     gene            complement(2781..3662)
                     /locus_tag="VFB52_09675"
     CDS             complement(2781..3662)
                     /locus_tag="VFB52_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZO06646.1"
                     /translation="MPDPHEIPLRSLLFAPAARTDLVAKLPRAGADLVTLDLEDGTAP
                     GQAEEARAGAATALPALAAAGQRAYVRTNPPDHDSFPADLAAVAPASPAGYVLPKCED
                     GDTVAAAAAAIAAAHGGEAPPLILGIETVAGVMEAPALAVSAPSVQALYFGAEDFATS
                     AGARRTEAGDEVLYARSRVLLAARAAGVRALDQAIPDVRDDARFAADAAAGRDLGYDG
                     KLCIHPRQVELSHEAFTPSAEEIAAAARLLDAFEAALAAGNATPLIDGRMVDEPLARR
                     ARALVASAGAEGVTSGN"
     gene            3702..4064
                     /locus_tag="VFB52_09680"
     CDS             3702..4064
                     /locus_tag="VFB52_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06647.1"
                     /translation="MSAPENAQIALRQVLLPTPDLDAALNFYEALGLTLKFRDGDRYA
                     ALDAGGATVALVGPEDRDPEAGVAPALKVDSVDALAAQLRAAGWAVGEPEDGGHERRL
                     ELRDPAGNPLLVYGPLPS"
     gene            complement(4061..4705)
                     /locus_tag="VFB52_09685"
     CDS             complement(4061..4705)
                     /locus_tag="VFB52_09685"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO06648.1"
                     /translation="MASTRSLRRQARTPLDADDPTKAALLESTERMLRERPLAQLTVD
                     QISRGANLTRTAFYRYFGRKEDAVVALSQRYFDLIFEGRKPFFSEDRPLEEACREAVE
                     NELSVWAEHGAVLRGLADLGAADPEMREAWYEQVEQFVTPVEKRIRSHAKQRGVKPPP
                     HPRQVAEAFIWMAERYYYVWSSHYRHPTEDVVEAMTALMVSMMNGAPAAGRGKA"
     gene            complement(4727..5569)
                     /locus_tag="VFB52_09690"
     CDS             complement(4727..5569)
                     /locus_tag="VFB52_09690"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZO06649.1"
                     /translation="MTLTPTTLLDGLVFPEGLRWHDGRLWLSDMYAGKVLAVDLDGAV
                     EEIAAVPGRPSGLGFLPDGTPLVVSMEDHKLLRLEPGGAVEHADLTSLVTGHINDMLV
                     TPSGRAYVGNFGFDFLGGEDPKPANVVVVEPDGSAQVAADGLMFPNGMALADGGATLL
                     VAEMLGGKVTAFAVAADGTLTDPRTWAELPERTPDGIALDADGNLWIASSLSAEALLV
                     APGGEVLELLSTAPRLAPACALGGPDGDTLFLALAETSPEQLAQGISKGQIDTVSVAV
                     AAAA"
     gene            complement(5566..>6976)
                     /locus_tag="VFB52_09695"
     CDS             complement(5566..>6976)
                     /locus_tag="VFB52_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZO06650.1"
                     /translation="SSAAEQIAYIAGHCEARVAIVEPAFLPRMLEARASLPGLERVVL
                     VGGGGEEDWLVDWSELLAADPADLEALAAGVRPEDLATIIYTSGSTGPPKGVMLTHSN
                     IAWQTAAMSRRIEEPMTGWRQISYLPMAHIGERMMGHYLAIRGGTEITCCPDPAAAGG
                     MLATVRPQIFLGMPRMWEKLHAELLAHAARNPDDCEREQLLSVVGLEGGRIMLTSGGV
                     LATEIHAFFRELGLPLSDIYGMSESTGPICWSPHDPKPGCVGYAMPGCELRLEADNEI
                     VFRSGCAFAGYLADPGKTAEIDAGDGWFRTGDVGALDDSGQLRIVDRKKEIMITAGGK
                     NISPANLEKALKAQPLIGQAVAIGEARRFVSALIVLDPLSAAEWAREQGLPADLPLAE
                     LVTLPGLLEAVGAAVEVANETFSQAEKVKRFTVLPTEWTPDSDELTPTAKLKRGAVAA
                     KYAEEIEAMYVEPRTKEPA"
BASE COUNT          927 a         2457 c         2648 g          944 t
ORIGIN      
        1 gatggtgatg gaccagacgc catcgttggc gtcgacgacg acttcgttca acagaggttc
       61 ctcgcagtct cggttagctc gtctcgaaaa gctgggtcgg cgatcgcgat caggcgccgg
      121 gcgcgctcgg gcaggaagca gccgcacagg tgggcggcgc cgtgctcggt gacgacgacg
      181 tcggcgtcgc tgcgcgcggt ggtgaccacg ccgtcggcga gccgggcgac gatccgcgag
      241 gcggtgccct tggcggcggt cgccggcagg gcgatgatcg agcggccgcc tgggctcgcc
      301 atcgccgcgc gcacgaagtc gacctgaccc ccgaccgcgc cgacgtggcg acccctgagc
      361 gtctccgcgt tcacctggcc ggtgagatcg acctcgagcg cgccgttgat cgcgacgaag
      421 gtgccgagct gggcgatcgt gcgcgggttg tgggtgtggg ccagggcgcg cacctcgagc
      481 tccgggtttt catcggcgaa gtcgtagagg cgctcggtgc cgaacagggc gccggccacg
      541 gagaccccgg tgtcgatcgg cttgcgcgcg ttggtcatcg ccccggaggc gatcagctcg
      601 accatctcgt cgccgaccgc gccggtgtgc aggccgaggt cgcggtggcc gcgcaggcag
      661 gcgaggatcg cgttgggcac ggtgccgaac ccgacctcca gggtggcgcc gtcggggacc
      721 agctcggcga cgtgggcggc gatcgcctcc tcgaccggcc cggggggcgg cgccgtgacc
      781 gagaccagcg gccggtcgct gtggacgaag cggtcgatct ggccgggctc gacccgggtg
      841 tcccccccgg tccgcggcat ccgctcgttg acctcggcga cgacgacccg cgccgcggcg
      901 atcgcctcgt gcacgtagtc ggcgatcacg ccgaggctgt gggagccgtc ctcgccggcc
      961 ggcgagacct ggatcaggac cacgtcggcg cggatcaggc cgcgctcgat caggccgggc
     1021 agggtcgaga cgtggcaggg gatcaccgcc agctccccgg gcggggcgag cctggtgttg
     1081 gcgccggcgc cgccgagcac ggtgaccgtg gtctggctga ggtcggcctc gtcgaaggtc
     1141 gtgctgaagg tgccgccgaa gaagacctcg atgcccggat aggcgggcag gtcgcgcacg
     1201 aacgcctcgg tcagggtgcg cggctccccg gtcccctgcg gccagatgac gccgtcgccg
     1261 ggctggatca cgtcggtcag gtcgaccgcg ggggtggcgg tggtcgcggc catcagtaga
     1321 gctggacccc ggggcccgag tggcggacca gcacggtctg caggtcgcgg tactcgtcga
     1381 gcgcggcggc gccgttctcg cggccgaagc cgctgtcctt gaccccgccg aagggcgcgc
     1441 ccgggggcag gaagccgtag gtgttgaccc aggcgacgcc ggcgtcgacg cgatcgagca
     1501 gggcgagagc gcggcccgcg tcggcgcacc agaccgcggc ggcaaggccg gtggcgacat
     1561 cgttggcgag ctcgggcagg gccttctcgt cctcccagcg caggaccgag agcaccgggc
     1621 cgaaaacctc ctcgcgggcg accttcatct ccggcgtcgc ctcgaagccg accaccgggc
     1681 accagttgcc gccctcggga aggccgtcgg gcgggtcgag ccggaagacc tcgcgggcgc
     1741 cagccgcggt cgcctcctcg atccgcgcca gcaccttctc gcgctggccg gcgctgacca
     1801 gcgggccgag gtcggaggtc gggtcgagcg gcgggccgat gcgcaggccg gcgatccgct
     1861 cgccgagctt ggcgaggaac tcgtcggcga tccgctcgtg cagcagcaac cgcgttccgg
     1921 ccgcgcacat ctgcccctgg ttgggaatga tcccgaccag ggtgccctcg accgcggcgt
     1981 cgaggtcggc gtcggggaag acgatgctcg ggctcttgcc gcccagctcc agcatcaccg
     2041 gggtcagccg ctcggcggcg gccgccatca cctgacggcc gacggcgggg ctgccggtga
     2101 agctgatccg gcgcagctcc ggcgcggaga cgatcgcgcg gccgacctcg ggcccgccgg
     2161 tgaccaggtt gacggtcccg ggcggcagcg cgtcggccca gacccgcagc agctccagcg
     2221 ccggcagcga ggccagctcg gagggcttca ggaccacggt gcagccggcg gcgagcgccg
     2281 gcgcgacctt catcgcgacc atcaacacgg tcgcgttcca ggggatgatc tggccgaccg
     2341 gcccggctgg gcggcggcgc acgatctgca gggtgccggc gttcggctcg gagacctcgc
     2401 cgccggaggt gcgggcgacg gcggcgtagt agcgcagcac ctcggcgcaa agcggcaggt
     2461 cccaggtggt cgcctggcgc accggcttgc cggcgtccca ggcctcgagc gcggccaggg
     2521 tcggcgcgtg cgcctgcacc cggtcggcga gctcgtgcag gagcgcggcg cgggcggcgg
     2581 gagaggagcg gcgccagcgc gggaaggcgg ccgcggcagc ggccacggcg gcctcgacgt
     2641 cggcggcgtc gccgtcggcg atctcggcga tctgggcgcc gttggcgggg tcgaccgcgg
     2701 ccagagcggg accgggcacg gtccactcgc caccgatcag gtggccccag ctgcgctcga
     2761 cgaccggtgc tgcgatcggt tcagttgcca ctggttactc cttctgctcc agcgctggcg
     2821 accagcgcgc gggcgcggcg ggcgagcggc tcgtcgacca tgcgaccatc gatcaggggg
     2881 gttgcgttgc cggcggcgag cgccgcctcg aaggcgtcga gcagccgcgc cgcggccgcg
     2941 atctcctccg ccgagggcgt gaacgcctcg tggctgagct cgacctggcg cgggtggatg
     3001 cagagcttgc cgtcgtagcc gaggtcacgg ccggcggcgg cgtcggcggc gaagcgggcg
     3061 tcgtcgcgga cgtcggggat cgcctggtcg agcgcccgca ccccggccgc gcgcgccgcc
     3121 agcagcaccc gcgagcgcgc gtagagcacc tcgtcgccgg cctcggtgcg gcgggcgccg
     3181 gcgctggtcg cgaagtcctc ggcgccgaag tagagcgcct gcaccgaggg ggcggagaca
     3241 gccagggccg gcgcctccat caccccggcg acggtctcga tcccgaggat cagcggcgga
     3301 gcctcacccc cgtgagcggc ggcgatcgcg gcggccgcgg cggcgacggt atcgccgtcc
     3361 tcgcacttcg gcagcacgta accggcggga gaggctggtg cgactgcggc gaggtcggcg
     3421 gggaacgagt cgtggtcggg gggattggta cgcacgtagg cgcgctggcc ggcggcggcg
     3481 agcgcgggca gcgccgtggc ggccccggcc cgggcctcct ctgcctgccc gggggcggtg
     3541 ccgtcctcga ggtcgagagt gaccagatcg gcacccgcgc ggggcagctt ggcgaccagg
     3601 tccgtgcgtg ctgcaggcgc gaaaagcaag ctgcgcagcg gaatctcgtg ggggtcgggc
     3661 actatgctgt atatattacg catgtttttg aaagagcaca aatgagcgcg cctgaaaacg
     3721 cccagatcgc gctccgccag gtcctccttc ccacccccga cctggacgct gcgctcaatt
     3781 tctacgaagc gctcggactt actttgaagt tccgcgacgg cgaccgctac gcggcgctcg
     3841 acgcgggcgg cgcgacggtc gccctggtcg gccccgagga ccgcgacccg gaggccggcg
     3901 tcgccccggc gctcaaagtc gactcggtcg acgcgctcgc cgcgcagctg cgtgccgccg
     3961 gctgggccgt cggcgagccc gaggacggcg gccacgagcg acggctcgag ctgcgcgacc
     4021 cggccggcaa cccgctgctg gtctacgggc ccctcccctc ttaggccttg ccgcgccccg
     4081 cggccggcgc cccgttcatc atcgagacca tcagcgcggt catcgcctcg accacgtcct
     4141 cggtcgggtg gcggtagtgg ctcgaccaca cgtagtagta gcgctcggcc atccagatga
     4201 acgcctcggc gacctggcgc gggtgcggcg gcggcttcac gccgcgctgc ttggcgtgcg
     4261 agcggatccg tttctcgacc ggggtgacga actgctcgac ctgctcgtac caagcctcgc
     4321 gcatctccgg gtcggcggcg ccgaggtcgg cgagcccgcg caacacggca ccgtgctcgg
     4381 cccagaccga gagctcgttt tcgaccgcct cgcggcaggc ctcttcgagc ggccggtcct
     4441 cggagaagaa cggcttgcgg ccctcgaaga tcaggtcgaa gtagcgctgg gagagcgcca
     4501 ccacggcgtc ctctttgcgg ccgaagtagc ggtagaacgc ggtccgggtg aggttcgccc
     4561 cgcggctgat ctggtcgacc gtgagctgcg ccagagggcg ctcgcgcagc atccgctcgg
     4621 ttgactcgag cagcgccgcc ttggtcgggt cgtcggcgtc gagcggcgtg cgcgcctggc
     4681 gccgcaggga gcgggtcgat gccatctatc gcaagctagc gctgatctag gcggcagccg
     4741 cgaccgcgac gctgaccgtg tcgatctggc ctttggagat gccctgcgcg agctgctcgg
     4801 gcgaggtctc ggccagggcg aggaagagcg tgtcgccgtc ggggccaccg agcgcgcagg
     4861 ccggcgccag ccgcggcgcg gtcgagagca gctccagcac ctcgccgccg ggggcgacca
     4921 gcagcgcctc ggccgagagc gaggaggcga tccagaggtt gccgtcggcg tcgagcgcga
     4981 tcccgtccgg ggtgcgctcg ggcagctcgg cccaggtccg cgggtcggtc agggtgccgt
     5041 cggcggcgac cgcgaacgcg gtcaccttgc ccccgagcat ctcggcgacg agcaaggtcg
     5101 caccgccgtc ggcgagcgcc atcccgttcg ggaacatcaa gccatcggcg gcgacctggg
     5161 cgctgccgtc gggctcgacc acgaccacgt tggccggctt cgggtcctcg cccccgagga
     5221 agtcgaagcc gaagttgccg acgtaggcgc ggccgctcgg ggtgaccagc atgtcgttga
     5281 tgtgaccggt caccagggaa gtcaggtcgg cgtgctcgac cgcgccgccg ggctccagcc
     5341 gcaacagctt gtgatcctcc atcgagacga ccagcggcgt gccgtcgggc aggaagccga
     5401 gccccgaggg ccggcccggc accgcggcga tctcctcgac cgcgccgtcg agatcgaccg
     5461 caagcacctt gcccgcgtac atgtcggaga gccagaggcg gccgtcgtgc cagcgcaggc
     5521 cctccgggaa gacgagcccg tcgagcagcg tggtcggggt gagggtcatg cgggctcctt
     5581 cgttcggggt tcgacgtaca tcgcctcgat ctcctccgcg tacttggcgg cgacggcgcc
     5641 gcgcttcagc ttcgccgtcg gggtcagctc gtcggagtcg ggggtccact cggtcggcag
     5701 gaccgtgaag cgcttcacct tctcggcctg ggagaaggtc tcgttggcga cctcgaccgc
     5761 ggcgccgacg gcctccaaca ggccgggcag cgtgaccagc tcggccagcg gcaggtcggc
     5821 cggcaacccc tgctcgcgcg cccactcggc ggcgctcagc ggatcgagca cgatcagcgc
     5881 cgagacgaag cgccgcgcct cgccgatcgc caccgcctgg ccgatcagcg gctgcgcttt
     5941 cagcgctttc tcgaggttgg cgggcgagat gttcttgccg ccggcggtga tcatgatctc
     6001 cttcttgcgg tcgacgatcc gcagctgccc ggagtcgtcg agcgcgccga cgtcgccggt
     6061 ccgaaaccag ccgtcgccgg cgtcgatctc ggcggtcttc cccgggtcgg cgaggtagcc
     6121 ggcgaaagcg cagccgctgc ggaagacgat ctcgttgtcg gcttccaggc gcagctcgca
     6181 gccgggcatc gcgtagccga cgcagccggg cttggggtcg tgcggggacc agcagatcgg
     6241 cccggtcgac tcgctcatcc cgtagatgtc ggagagcggc agcccgagct cacggaagaa
     6301 ggcgtggatc tcggtcgcca gcacgccgcc gctggtcagc atgatccgcc cgccctcgag
     6361 cccgaccacg gagagcagct gctcgcgctc gcagtcgtcg gggttgcggg ccgcgtgggc
     6421 gagcagctcc gcgtgcagtt tctcccacat ccgcggcatc ccgaggaaga tctgcgggcg
     6481 caccgtggcc agcatcccgc cggccgccgc cgggtcgggg cagcaggtga tctcggtgcc
     6541 gccgcggatc gccaggtagt gacccatcat ccgctcgccg atgtgggcca tcggcaggta
     6601 ggagatctgg cgccatccgg tcatcggctc ctcgatccgc cgcgacatcg cggcggtctg
     6661 ccaggcgatg ttggagtggg tcagcatcac gcccttcggc gggccggtcg aaccggaggt
     6721 gtagatgatc gtcgccagat cctcggggcg gacgccggcg gcgagcgcct cgaggtcggc
     6781 cgggtcggcg gcgagcagct cgctccagtc gaccagccag tcctcctcgc cgccaccgcc
     6841 gaccaggacc acgcgctcca gcccgggcag gctcgcccgc gcctccagca tgcgcggcag
     6901 gaaggccggt tcgacgatcg ccacgcgggc ctcgcagtgg ccggcgatgt aggcgatctg
     6961 ctcggcggcg gaggag
//
LOCUS       DASRIG010000361         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2248652, whole genome shotgun sequence.
ACCESSION   DASRIG010000361 DASRIG010000000
VERSION     DASRIG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248652"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1898
                     /locus_tag="VFB52_09700"
     CDS             135..1898
                     /locus_tag="VFB52_09700"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZO06651.1"
                     /translation="MRRFVPPLAVALVLLAFAAGASGAARLYAPSYGSGPPEVIGAFE
                     RADNGSLTPVAGSPFAAYPGPPYAVGGLIGFGFGPDGSKAVAGFLFDGGVQGMTVGAG
                     GQITPTNSVVTRQSEGIAVSPDGRFAYEATREYPPGTESQGINVFAIGADGALSPFAE
                     SFDVGTSFSQVAIAPNGRFLFATTGSGVKRFAIEPDGGLSYLGTTSLGTFGIWPTTSP
                     DGRFLFVGDAGGKGGVYSFTIGADGSLSPNGESALTGGSAVEAFSVSPDGKFLYMPDE
                     GQQKIVVASVAADGSLSVIGSIPVEDPEVSAVSPDGRYLYWQHGGETEGIAVAALGAD
                     GMPVVLPFNAPWEVDEPVRFAFQPGPAPVAAFKAKAAAPGAAATFNAGSSTNAAHYSW
                     NFGDGTTLAEGGPKPSHAYKKAGRYTVTLAVTDGQGCGAQQIYTGQSTTCPGGTATTT
                     TAVVDTLPVVSKLKAQPKKFLPKGVGAKGKAGTTFRYALNEKAKVNLKIERKLPGRLV
                     GRKCKKPTSANKGRKKCSRFVKAGSRKGTGKVGINKVSFNGKLKGRPLLPGAYRLTAV
                     ATDSAQGRSAPRTAAFRVLPY"
     gene            complement(1834..2520)
                     /locus_tag="VFB52_09705"
     CDS             complement(1834..2520)
                     /locus_tag="VFB52_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZO06652.1"
                     /translation="MWDSLTRTLLALTFVTGILDAVSFLALGSVFAAMQTGNVIFLGL
                     GIGNAPGAPLVAPLIALAAFVAGGMVAALLARPNSPGAAGLRFALAVEIALLACAALL
                     AALIDVEPEQASAYVLIALLSLAMGLRNTIARRVGDPNLATTVLNLTLTAFVTHTPTG
                     FASQTELGVRIAAIAAIVTGAVAGALLLKASLALAIAAAALLVLGVALSTGGRGRRPS
                     WGRSAPAPSR"
     gene            2589..3452
                     /locus_tag="VFB52_09710"
     CDS             2589..3452
                     /locus_tag="VFB52_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO06653.1"
                     /translation="MKKLYATLAFVLVLGLLVAGCGSSDDDSSGTEGGTTATVDCKPG
                     ALETQSDGVLTIATDKPAYPPYFEDDDPTNGEGFESAVAYAIADQLGYPAAKVEWTVE
                     PFNSSYAPGPKDFDFDVNQISITPAREKAVDFSAPYYVANQAVLALKDSDAAKATSLE
                     ELQDAQLGVQIGTTSLEAAEEVIEPSSKPEVFNNSTDVTTALKNGQIDAIIVDLPTAL
                     YLTAVEIPEATVVGQFSAPGGDQWGALLAKESALTECVSQAVEELESSGELEEITTKW
                     MSTAAGAPVLE"
BASE COUNT          603 a         1267 c         1186 g          506 t
ORIGIN      
        1 gacccattcc tgcagcttga ccagctcgac ctgcaggcgc tccagctcct tttcgtaccc
       61 cttcttggag agctcgcggg gcgcctgatc cgtgtccgcc atcgtccgac cctactggct
      121 aatctgccgc ctgcatgcgt cgttttgtcc ccccgctggc cgttgccctg gtcctgctcg
      181 cgttcgcggc gggtgcgtcg ggcgccgccc gcctctacgc gcccagctac ggctccggac
      241 cgccggaggt gatcggtgcc ttcgaacgcg ccgacaacgg ttcgctgacg ccggtcgccg
      301 gctcgccctt cgccgcctac cccgggcccc cgtacgcggt cggcggtctg atcggctttg
      361 gctttggccc ggacggctcc aaggcggtcg ccggctttct cttcgacggc ggcgtgcagg
      421 gcatgacggt cggtgccggt gggcagatca cgccgaccaa ctccgtcgtt acccggcagt
      481 cggaaggaat cgccgtctcg cccgacggtc gcttcgccta cgaggccacg cgcgaatacc
      541 cgccggggac cgaatcgcag gggatcaacg tcttcgcgat cggcgccgac ggcgccctct
      601 ccccgttcgc cgagtccttc gacgtgggta cgtcgttcag ccaggtggcg atcgcgccga
      661 acgggcgctt cctcttcgcc accaccggct ccggggtcaa acgcttcgcg atcgagcccg
      721 acggcgggct gtcctatctc ggcaccacct cgctcgggac cttcggcatc tggccgacca
      781 cctcgccgga cgggcgcttt ctcttcgtcg gtgacgccgg gggcaaaggc ggggtctact
      841 cgttcacgat cggtgccgac ggctcgctca gcccgaacgg cgaatcggcg ctgaccggcg
      901 gcagcgccgt cgaggcgttt tccgtcagcc ccgacggcaa gttcctctac atgccagacg
      961 aggggcagca gaaaatcgtc gtcgccagcg tcgccgcgga cggctcgctg agcgtgatcg
     1021 gctcgatccc ggtcgaggac cccgaagtct cggcagtctc gcccgacggc cgctacctct
     1081 actggcagca cggcggcgaa accgaaggga tcgcggtcgc cgcgctcgga gccgacggga
     1141 tgccggtggt cctgcccttc aacgcgccgt gggaagtgga cgagccggtg cgcttcgcct
     1201 tccagcccgg ccccgccccg gttgccgcct tcaaagcgaa agccgccgcg cccggtgccg
     1261 cggccacctt caacgccggc agctcgacca acgccgcgca ctacagctgg aacttcggcg
     1321 acggcacgac gctcgccgaa ggcggtccga aaccgagcca cgcctacaaa aaagccggca
     1381 ggtacacggt caccctcgcg gtcaccgacg gccagggctg cggcgcccag cagatctaca
     1441 ccggccagag cacgacttgc cccggcggca cggcgacgac cacgaccgcg gtggtcgaca
     1501 ccctgccggt tgtcagcaag ctgaaggcgc agccgaagaa gttcctcccc aagggcgtgg
     1561 gggcgaaggg caaggcgggc acgaccttcc gctacgcgct gaacgagaag gcgaaggtga
     1621 acctgaagat cgagcgcaag ctgcccggcc gcctggtcgg ccgcaagtgc aagaagccga
     1681 cgagcgcgaa caaaggccgc aagaagtgca gccgcttcgt caaggccggc tcaagaaaag
     1741 gcaccggcaa ggtcgggatc aacaaagtga gcttcaacgg caagctcaaa gggcggccgc
     1801 tgctcccggg cgcctatcgg ctgaccgcgg tcgctaccga ctcggcgcag gggcgctccg
     1861 cccccaggac ggccgccttc cgcgtcctcc cgtactgagc gcgaccccga gcacgagcag
     1921 cgccgcggcg gcgatcgcca gcgccaggct cgccttcagc agcagcgccc cggcgacggc
     1981 gccggtgacg atcgcggcga ttgcggcgat ccggacgccg agctcggttt gcgaggcgaa
     2041 cccggtcggg gtgtgggtga cgaaggcggt cagggtgagg ttgagcaccg tggtcgcgag
     2101 gttcgggtcg ccgacccggc gcgcgatcgt gttgcgcagc cccatcgcca gcgagagcag
     2161 cgcgatcagc acgtaggcgc tcgcctgctc cggttcgacg tcgatcagcg ccgccagcag
     2221 cgccgcgcag gcaagcagtg cgatctcgac cgcgagcgcg aagcgcagtc cggcggcgcc
     2281 gggcgagttc ggccgcgcca gcagcgccgc gaccatgccg ccggcgacga aggcggcgag
     2341 cgcgatcagc ggcgcgacca gcggagcgcc gggcgcgttg ccgatcccga ggccgaggaa
     2401 gatcacgttg ccggtctgca tcgccgcgaa cacggagccg agcgcgagga agctgaccgc
     2461 gtcgaggatc ccggtgacga aggtgagggc aagcagggtt cgcgtcagcg agtcccacat
     2521 atgtacccat ttgatctagg atgcccgctt ccttcgcggt ttccgcgaat tcgcatcaag
     2581 ccaccttcgt gaagaagctc tacgccaccc tcgctttcgt cctcgtgctc ggcctgctcg
     2641 tggccggctg cggctcctcc gacgacgact ccagcggcac ggaaggcggc accacggcga
     2701 cggtcgactg caaaccgggc gccctcgaaa cccagtccga cggcgtgctc acgatcgcga
     2761 ccgacaaacc ggcctacccg ccgtacttcg aagacgacga cccgaccaac ggcgaaggct
     2821 tcgagagcgc ggtcgcctat gcgatcgccg accagctcgg ctacccggcg gcgaaagtgg
     2881 agtggacggt ggagccgttc aactcctcct acgcgcccgg cccgaaggac ttcgacttcg
     2941 acgtcaacca gatctcgatc accccggcgc gcgaaaaggc ggtcgacttc tcggcgccct
     3001 actacgtcgc caaccaggcg gtgctggcgc tgaaggactc cgacgccgcg aaagcgacct
     3061 cgctggaaga actgcaggac gctcagctcg gcgtccagat cggcacgacc agcctcgaag
     3121 cggccgaaga agtgatcgaa ccgtcctcga aaccggaagt cttcaacaac tccaccgacg
     3181 tcaccacggc gctcaaaaac ggccagatcg acgcgatcat cgtcgacctc ccgaccgcgc
     3241 tctacctgac cgcggtggaa atccccgagg cgaccgtggt cggccagttc agcgcccccg
     3301 gcggcgacca gtggggtgcc ctgctggcca aagaatcggc cctgaccgag tgcgtctcgc
     3361 aggcggtcga agagctcgaa tcatccggtg aactcgaaga gatcacgacc aaatggatga
     3421 gcaccgcggc gggcgcgccg gtgctcgaat aacgaactgg ggaccagggt tttgcgggga
     3481 ggggcggtga gggcctctcc gggaattggg ttgtgcgggc tggcgcccgg cacaactcca
     3541 gattgagccc gcctatggcg gg
//
LOCUS       DASRIG010000362         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3282665, whole genome shotgun sequence.
ACCESSION   DASRIG010000362 DASRIG010000000
VERSION     DASRIG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282665"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..294)
                     /locus_tag="VFB52_09715"
     CDS             complement(22..294)
                     /locus_tag="VFB52_09715"
                     /inference="COORDINATES: protein motif:HMM:NF039961.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6027 family protein"
                     /protein_id="HZO06654.1"
                     /translation="MAVPPEHQRFADQVAAAKLQDPMPTFANLSAATGVPVDDLVHFA
                     LVRWAASGSEALMAVEPLALRQLVEAREREDWEAVAGIVDWLASRA"
     gene            383..1909
                     /locus_tag="VFB52_09720"
     CDS             383..1909
                     /locus_tag="VFB52_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06655.1"
                     /translation="MAGGAASAVIRSIEFHARRDPGHECLVDLRPGEAARRFSYAEIE
                     AAIAKLAGGLVAEGVRPGDRVVLRLQNTSDFVVALFACLRAGAIAVPTITQYAGEELR
                     YAVEDCGARCLIFDDPDLEVVGEALVGGVAAFAAGAATGPGIRPLAELAEAAPAPAVP
                     VVEDGDALIFYTSGTTAQPKGVVLSHAAIAGASLINAEGWRLRPDDRSYIVLPLFHCN
                     ALFMQLIPALLSGATAILGERYSASAYLDEAREHGITLANLTAGAIRSLLAQPESDRD
                     REHRLRQLTFGLPLHREEIERIEARFGIPVYMCFGLTETSSGGTRSPVHVDPKSGWQS
                     LGVAQPGWQVMIAGEDGPAAAGEVGEILFRGPGVMSRYWERPELTEVALADGWLHTGD
                     LGTLDADGYLFFHSREKDMLKPKGENVAASEIEAALEEHEAIAEAGVVGVFDPHHEER
                     IVAMVVGSEVGEEELREHCLERLAAFKVPKEFVWVEELPKTSIGKINKGAIKSAVEGR
                     "
     gene            1912..2679
                     /locus_tag="VFB52_09725"
     CDS             1912..2679
                     /locus_tag="VFB52_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06656.1"
                     /translation="MSEGRMRGRVAVITGAAGGLGRASAERVLEEGGAVALWDLDGAR
                     VERVGAELAELGPTHALAVDVGDSAAVAAAAASSRATLGPIDSLVNNAGYVVGTPPWE
                     VSDQEWERHFRVNADGAFWCVRACLPDMRERRYGKIVNIGSQAAQQGRPTTSPAYASS
                     KGAILGLTVSLARNLGEYGICVNAVNPGFIRTEIHDQFSDEQIAELTADIPLHRDGVK
                     GKGLPVDIANAVLFLLSSESDYISGAFLGVNGASRTA"
     gene            2835..>4171
                     /locus_tag="VFB52_09730"
     CDS             2835..>4171
                     /locus_tag="VFB52_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO06657.1"
                     /translation="MSGYRISVDIGGTFTDCTVVAEDGTVTIGKSPSTPPEFATGFVN
                     SVAAAAEAMGLSLQELLAQTGRLSHGTTVGINAVVSRTGARVGLISTVGHGDSLRILN
                     NTGRTNGQPVERILDYASSSLPERFVRREDVAEVSERIDSFGEIVVPLDRDGVLRAAE
                     GLLDQGCETLALSFLWSHVNPVHEDAARELLSERYPDLYISYGALLAQRIGEYPRTAT
                     AVLNSYIGPLMRDYVTTLIDELRGNGYSDRLLFAQCDGGLIDAEDVIARPVMTLESGP
                     VGGVVACAKAGSERGQANIIAADMGGTTFDVSVISDSQLPIRDSVVVEQHDLFLRMVD
                     VESVGAGGGSLAWVDPQSGALRVGPQSAGADPGPVCYGRGGTVPTVTDADLVLGLLNP
                     DNFLGGRMTLDVAAARAAIGALGEQLGLGLLECAAGVQEVADSLMEDKIRSMTV"
BASE COUNT          594 a         1512 c         1483 g          582 t
ORIGIN      
        1 cagatccgcc acgccgcaat gctacgcgcg gctggcgagc cagtcgacga ttccggcgac
       61 cgcctcccag tcctcgcgct cgcgagcttc gacgagctgg cgcagcgcca gcggctcgac
      121 cgccatcagc gcctccgacc cgctcgccgc ccagcgcacc agcgcgaagt ggacgaggtc
      181 gtcgaccggc accccggtcg ccgccgagag gttggcgaag gtcggcatcg ggtcctgcag
      241 cttggccgcc gccacctggt cggcgaagcg ctggtgctcg ggcggaacgg ccattggcga
      301 actactagcc gatctgcggg ttggaagcgg ttcggcgcct cggtagggat gcggtgtccg
      361 gattcttcga ggagggatgt ccatggctgg tggcgctgcg tcggcagtga tcaggtcgat
      421 cgagttccat gctcggcgcg acccggggca cgagtgcctg gttgatctgc ggccgggtga
      481 ggcggcgcgg cggttcagct acgcggagat cgaggcggcg atcgcgaagc tggccggcgg
      541 gctggtcgcc gagggggtgc ggccggggga ccgggtcgtg ctgcggctgc agaacaccag
      601 cgacttcgtc gtcgccctgt tcgcctgcct gcgcgccggc gcgatcgcgg tgccgacgat
      661 cacccagtac gcgggcgagg agctgcgcta cgcggtcgag gactgcgggg cgcgctgcct
      721 gatcttcgac gacccggacc tggaggtggt cggcgaggcg ctcgtcggcg gtgtcgctgc
      781 gttcgccgct ggtgccgcca ccggtcccgg catccgcccg ctcgctgagc tggccgaggc
      841 cgcgcccgcc cccgcggtgc cggtggtcga ggacggcgac gcgctgatct tctacacctc
      901 cggcaccacc gcgcagccga agggcgtcgt gctcagccac gcggcgattg ccggcgcttc
      961 gctgatcaac gccgagggct ggcgcctgcg ccccgacgac cgcagctaca tcgtcctgcc
     1021 gctcttccac tgcaacgccc tcttcatgca gctgatcccg gccctgctca gcggtgccac
     1081 cgcgattctc ggcgagcgct acagcgccag cgcctacctc gacgaggcgc gcgagcacgg
     1141 gatcacgctc gccaacctga ccgcgggcgc gatccgcagc ctgctcgccc agcccgagtc
     1201 cgaccgcgac cgcgagcacc ggctgcgcca gctcaccttc ggcctgccgc tgcaccggga
     1261 ggagatcgag cggatcgaag cgcgcttcgg gatccccgtc tacatgtgct tcgggctgac
     1321 cgagacctct tccggcggca cccgcagccc cgtccacgtc gacccgaaga gcggctggca
     1381 gtcgctcggc gtcgcccagc ccggctggca ggtgatgatc gccggcgagg acggtccggc
     1441 cgctgcgggg gaggtggggg agatcctctt tcgcggcccc ggggtgatga gccgctactg
     1501 ggagcgcccg gagctgaccg aggtggcgct cgccgacggc tggctgcaca ccggcgacct
     1561 cggcacgctc gacgccgacg gctacctctt cttccacagc cgcgagaagg acatgctgaa
     1621 gccgaagggc gagaacgtcg ccgccagcga gatcgaggca gcgctggagg agcacgaggc
     1681 gatcgccgag gccggcgtgg tcggcgtctt cgacccccac cacgaggaac ggatcgtggc
     1741 gatggtcgtc ggcagcgagg tcggcgagga ggagctgcgc gagcactgcc tggagcggct
     1801 cgccgccttc aaggtgccga aggagttcgt ctgggttgag gagctgccga agacctcgat
     1861 cgggaagatc aacaagggcg cgatcaaaag cgccgtggaa gggcggtagc gatgagcgag
     1921 ggacggatgc gcggccgggt cgcggtgatc accggcgcgg ccggcggcct cggccgggcg
     1981 agcgccgagc gggtgctcga agagggcggc gcggtggcgc tctgggacct cgacggcgcg
     2041 cgggtcgagc gggtcggcgc tgagctggcc gagctcggcc cgacccacgc gctcgcggtc
     2101 gatgtcggcg actccgccgc ggtcgcggcc gccgccgcga gcagccgcgc cacgctcggc
     2161 ccgatcgact cgctggtcaa caacgccggc tacgtggtcg gcaccccgcc ctgggaggtc
     2221 tccgaccagg agtgggagcg ccacttccgg gtcaacgccg acggcgcctt ctggtgcgtg
     2281 cgcgcctgcc tgcccgacat gcgcgagcgc cgctacggca agatcgtcaa catcggctcg
     2341 caggccgccc agcagggccg gccgacgacc agccccgcct acgcgtcctc gaagggcgcg
     2401 atcctcggcc tcaccgtctc gctcgcccgc aacctcggcg agtacgggat ctgcgtcaac
     2461 gcggtcaacc ccggcttcat ccgcaccgag atccacgacc agttctcaga cgagcagatc
     2521 gccgagctga ccgctgacat tcccctgcac cgcgacgggg tgaagggcaa ggggctgccg
     2581 gtcgacatcg ccaacgccgt gctcttcctg ctctccagcg agtccgacta catcagcggt
     2641 gccttcctcg gcgtcaacgg ggcctcgcgc acggcctagc cggacctggc ggttccgaca
     2701 gaccttggcc cgaccgctgg ccacccgcgg aggtgcgaga taaggtcgcc gcctaccgcg
     2761 cggtaggcga tttctgggga ggagacgccg ccgccggagc tgtttgacga ccacgggaaa
     2821 ggacggatct ccaggtgagc ggttaccgga tcagcgtcga tatcggcggc accttcacgg
     2881 actgcaccgt cgtcgccgag gacggcacgg tgacgatcgg caagtcgccc tcgaccccgc
     2941 ccgagttcgc gaccgggttc gtcaactcgg tcgccgccgc ggccgaggcg atggggctct
     3001 cgctgcagga gctgctggcc cagaccggcc gcctctcgca cgggaccacg gtcgggatca
     3061 acgccgtcgt ctcccgcacc ggcgcccgcg tcggcctgat ctccacggtc ggacacggcg
     3121 actcgctgcg catcctcaac aacaccggcc gcaccaacgg ccagccggtc gagcggatcc
     3181 tcgactacgc ctccagctcc cttcccgagc gcttcgtgcg ccgtgaggac gtcgccgagg
     3241 tgagcgagcg gatcgactcc ttcggcgaga ttgtcgttcc gctcgaccgc gacggcgtcc
     3301 tgcgcgccgc cgaggggctg ctcgaccagg gctgcgagac gctcgcgctc agcttcctct
     3361 ggagccacgt caacccggtc cacgaggacg ccgcgcggga gctgctcagc gagcgctacc
     3421 ccgacctcta catcagctac ggcgccctgc ttgcgcaacg gatcggcgag tacccgcgca
     3481 ccgcgaccgc cgtcctcaac tcctacatcg gcccgctgat gcgcgactac gtcaccactc
     3541 tgatcgacga gctgcgcggc aacggctact ccgaccgcct gctgttcgcc cagtgcgacg
     3601 gcggcctgat cgacgccgag gacgtgatcg cgcggccggt gatgaccctg gagtcggggc
     3661 cggtcggcgg cgtcgtcgcc tgcgccaagg ccggcagcga gcgcggccag gccaacatca
     3721 tcgccgccga catgggcggc accaccttcg acgtcagcgt gatctccgac tcgcagctgc
     3781 cgatccgcga cagcgtcgtg gtcgagcagc acgacctctt cttgcgcatg gtcgacgtcg
     3841 agtcggtcgg cgccggcggc ggcagcctcg cctgggtcga tccccagtcg ggcgcgctgc
     3901 gggtcggccc gcagagcgcc ggcgccgacc ccggaccggt ctgttacggg cgcggcggca
     3961 cggtgccgac ggtcaccgac gccgacctcg tgctcgggct gctcaacccg gacaacttcc
     4021 tcggcggccg gatgacgctc gacgtcgcgg ccgcccgggc ggcgatcggc gcgctcggcg
     4081 agcagctcgg cctcggcctg ctcgagtgcg cggccggcgt ccaggaggtc gccgactcgc
     4141 tgatggagga caagatccgc agcatgaccg t
//
LOCUS       DASRIG010000363         5948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1227266, whole genome shotgun sequence.
ACCESSION   DASRIG010000363 DASRIG010000000
VERSION     DASRIG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227266"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFB52_09735"
     CDS             complement(<1..255)
                     /locus_tag="VFB52_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HZO06658.1"
                     /translation="MYLNRLSLSATVHCLTGCAIGEVLGLALATWWGWHDVASIALAV
                     ALAFFFGYLLTSLPLLRSGLTVREAAPLAFASDTASIATME"
     gene            complement(255..566)
                     /locus_tag="VFB52_09740"
     CDS             complement(255..566)
                     /locus_tag="VFB52_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZO06659.1"
                     /translation="MAATESNESSSPTRGYTASKDQLLKRLRRVEGQVRGIEGMVEDD
                     RYCIDVLTQISAVQAALDKVALGLLDEHARHCVMGAEGQLQADRTDELMSAVGRLMRR
                     G"
     gene            669..881
                     /locus_tag="VFB52_09745"
     CDS             669..881
                     /locus_tag="VFB52_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZO06660.1"
                     /translation="MAEQRVRQYTVTGMTCDHCVTSVREEVGEVAGVSAVDVDLASGS
                     LRVSGAAFEDPAVEAAVAEAGYEVAR"
     gene            878..1804
                     /locus_tag="VFB52_09750"
     CDS             878..1804
                     /locus_tag="VFB52_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06661.1"
                     /translation="MSGGARLAVFAVGLVALAAIGFGIGALVDADHMGGDSGMDAMHD
                     GEGGHGTGPAMHGDQGGGGMHAQGGHGAMQMDAVRGLGVAENGMRLVVADPEFARGTT
                     EQLSFQVVGNDGEPLTAFEVTHTKRMHVIVVRRDLTDFQHLHPEMAADGTWSVPLRLA
                     EAGTYRVFADFSTDGEPTTLADDLRVDGDADLRDLPAPATRALSEPGDYEVSLRAGDP
                     HAGEEASLRFAIGRDGQPASLQPYLGAGGHLVALREGDLTFLHVHPTGDAGEDGSIEF
                     EATFPTAGNYRLFLQFVVGGELQTVAFTEEVH"
     gene            1804..4071
                     /locus_tag="VFB52_09755"
     CDS             1804..4071
                     /locus_tag="VFB52_09755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZO06662.1"
                     /translation="MSAVTERSGERVELPITGMTCASCANRIERKLNKLDGVSATVNY
                     ATEVAAVEFDPALVEPEALVETVEAAGYETALPAADGAGSADREAGEDDETAPLRRRL
                     LISAALSLPVLVLSMIPALQFDNWQWLALQLATPVVLWGAWPFHRAAWANLRHGTATM
                     DTLISLGVLAAWGWSLYALFIGDAGVTGMTMEFQLLPNGSGGDSEIYLETAAVVTTFI
                     LAGRYFEARAKRRAGAALRALLELGAKEVSVLDAEGREQRLPIEQLAVGDRFLVRPGE
                     KVATDGVVEEGRSAIDQSLLTGESVPVEVAPGDEVAGASVNAGGRLVVRATRVGADTA
                     LAQIARLVSDAQNGKAPVQRLADRISGVFVPAVIGLAVATLAFWLGAGEGASFAFTAA
                     VAVLIIACPCALGLATPTALMVGTGRGAQLGLLIKGPEVLESSRRVDTIVLDKTGTVT
                     TGRMELAEVAVTHGGEGRREALRLAGALEDASEHPIARAIAAAARAEGIELPPVEDFR
                     NREGLGVEGVVDGHGVQVGRASLLAEWSLAIPEQLAAARAQAEARGATPVLVAWDGAA
                     RAVLVVADTVKPTSAEAVAALKGLGIRPLLLTGDNEATARAVAAEVGIDEVIAEVLPA
                     DKAAVVARLQAEGRVVAMVGDGVNDAPALARADLGLAIGTGTDVAIEASDLTLVSGDL
                     RAAADAIRLSRSTLRTIRENLAWAFGYNVAALPLAAAGLLNPVIAGAAMALSSVSVVA
                     NSLRLRRFRKVGGDE"
     gene            4064..4732
                     /locus_tag="VFB52_09760"
     CDS             4064..4732
                     /locus_tag="VFB52_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZO06663.1"
                     /translation="MSEETHGAVDAFVVETIAADDEALSAALADATAAGLPEIAVTPP
                     QGRLLTLLARSVGARTILEFGTLGGYSTIHLARALPDDGRLISLEAKPEFAEVARASI
                     ERAGLGDKVEIRVGPALESLPALDEEGVGPFDFTFIDADKVNTPHYFAWALENSRPGS
                     LILADNVVRGGTLADAEDPDPATKAQRSFHETLAEEPRVFATTIQTVGAKGHDGFTLA
                     LVES"
     gene            4868..4981
                     /gene="rpmJ"
                     /locus_tag="VFB52_09765"
     CDS             4868..4981
                     /gene="rpmJ"
                     /locus_tag="VFB52_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZO06664.1"
                     /translation="MKVRASVKPMCEKCKIIRRNGAVLVICQNPRHKQRQG"
     gene            4985..5356
                     /gene="rpsM"
                     /locus_tag="VFB52_09770"
     CDS             4985..5356
                     /gene="rpsM"
                     /locus_tag="VFB52_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZO06665.1"
                     /translation="MARISGVNLPVNKRVEIGLTYIFGVGQSTAKQILAETKIDPGTQ
                     VKDLTEDEVIKLRDAVDNREVEGDLRRERSQNVKRLMEIGSYRGLRHRRGLPVRGQRT
                     KTNARGRKGPRRLSIAGKRKV"
     gene            5367..5765
                     /gene="rpsK"
                     /locus_tag="VFB52_09775"
     CDS             5367..5765
                     /gene="rpsK"
                     /locus_tag="VFB52_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZO06666.1"
                     /translation="MAEKKPSKGRSRRRVKKNVTYGQAHIKTSFNNTIVTLTDTDGNV
                     IAWESAGSAGFKGSRKSTPFAAQVTADAAAKKGMEHGLERVEVFAKGAGSGRETAIRS
                     LQAAGLEVTTVKDVSPQAHNGCRPKKRRRV"
     gene            5766..>5948
                     /locus_tag="VFB52_09780"
     CDS             5766..>5948
                     /locus_tag="VFB52_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZO06667.1"
                     /translation="MARDTAPQCKQCRREGQKLFLKGERCFTDKCGVERRSYPPGEHG
                     RGRLRQSEYRLQLREKQ"
BASE COUNT          882 a         2060 c         2161 g          845 t
ORIGIN      
        1 ctccatcgtc gcgatcgagg cggtgtcgga ggcgaaggcg agcggcgcgg cctcgcgcac
       61 ggtcaggccc gagcgcagca gcggcaggct ggtcagcagg tagccgaaga agaaggcgag
      121 ggcgaccgcg agcgcgatcg aggcgacgtc gtgccagccc caccaggtcg ccagggcgag
      181 gccgagcacc tcgccgatcg cgcagccggt gaggcagtgg acggtggcgc tgagcgagag
      241 ccggttgagg tacatcagcc tctccgcatc agccgcccga cggcgctcat cagctcgtcg
      301 gtgcggtcgg cctggagctg gccctctgcc cccatcacgc agtggcgggc gtgctcgtcg
      361 agcagcccca gcgcgacctt gtcgagcgcg gcctgaacgg cggagatctg ggtcagcacg
      421 tcgatgcagt agcggtcgtc ctcgaccatg ccctcgatcc cgcgcacctg gccctcgacc
      481 cggcgcagcc gtttgagcag ctggtccttt gaggcggtgt agccgcgggt gggggaactg
      541 ctctcgttgc tctcggtggc tgccatctgg gctcctcgct cgctctcggt cgggggattc
      601 agataccccc tatgggtata tgctagcatc gcgatcagcg atacccccct aaggtatagg
      661 agagagcgat ggccgaacag cgagtgcgcc aatacaccgt caccgggatg acctgcgatc
      721 actgcgtcac ctcggttcgc gaggaggtgg gggaggtcgc aggcgtgagc gccgtcgacg
      781 tcgatctggc cagcgggagc ctgcgggtca gcggcgccgc cttcgaggac cctgcggtcg
      841 aggccgcggt cgccgaggcc ggctacgagg tggcccggtg agcggcggcg cccgcctcgc
      901 cgtcttcgcg gtcgggctcg tcgcgctcgc ggccatcggc ttcggcattg gggccctggt
      961 cgacgccgac cacatgggcg gcgactccgg gatggacgcg atgcacgatg gcgagggcgg
     1021 gcacgggacc ggtccggcga tgcacggcga tcaaggcggc ggtgggatgc acgcccaagg
     1081 cgggcacggc gcgatgcaga tggacgcggt gcgcggcctc ggcgtcgccg agaacgggat
     1141 gcgcctggtg gtcgccgacc ccgagttcgc ccgcgggacg accgagcagc tgagcttcca
     1201 ggtcgtcggc aacgacggcg agccgctgac cgcgttcgag gtcacccaca ccaagcggat
     1261 gcacgtgatc gtcgtccgcc gtgacctcac cgacttccaa cacctgcacc cggagatggc
     1321 cgccgacggc acctggtcgg tgccgctgcg cctcgccgag gcgggcacct acagggtctt
     1381 cgccgacttc tccaccgacg gcgagccgac gacgctggcc gacgacctgc gcgtcgacgg
     1441 cgacgcggac ctgcgcgatc tgccggcccc cgcgacgcgg gcgttgagcg agccgggcga
     1501 ctacgaggtc tcgctgcggg ccggcgatcc gcacgcgggc gaggaggcga gcctgcgctt
     1561 cgcgatcggc cgcgacgggc agcccgcgtc cctgcagcct tacctcggcg ccggcggcca
     1621 cctcgtcgcc ctgcgcgagg gcgatctcac cttccttcac gtccacccga ccggagacgc
     1681 cggcgaggat ggctcgatcg agttcgaggc aaccttcccg accgccggca actaccggct
     1741 cttcctccag ttcgtggtcg gtggcgagct gcagacggtc gccttcaccg aggaggtgca
     1801 ctgatgtcgg ccgtgaccga gcgttcgggc gagcgggtcg agctgccgat caccggcatg
     1861 acttgtgcct cgtgcgcgaa ccggatcgag cgcaaactga acaagctcga cggggtcagc
     1921 gccacggtca actacgcgac cgaggtcgcc gcggtcgagt tcgacccggc gctggtcgag
     1981 cccgaggcgc tggtcgagac ggtcgaggcg gccggttacg agaccgcgct gccggccgca
     2041 gacggagccg ggtccgctga tcgcgaggcg ggggaggacg acgagaccgc gccgctgcgc
     2101 cggcgcctgc tgatcagcgc cgcgctcagc ctgccggtgc tggtcctctc gatgatcccg
     2161 gcgctgcagt tcgacaactg gcagtggctg gcgctgcagc tagccacgcc ggtcgtgctc
     2221 tggggcgcct ggccgttcca ccgggcggcc tgggcgaacc tcaggcacgg gacggcgacg
     2281 atggacacgc tgatctcgct cggcgtgctc gccgcctggg gctggtcgct ctacgcgctc
     2341 ttcatcggcg acgccggggt caccgggatg acgatggagt tccagctgct gcccaacggc
     2401 agcggcggcg acagcgagat ctacctggag acggcggccg tcgtcaccac cttcattctc
     2461 gccggccgct acttcgaggc gcgcgccaag cgccgcgccg gcgccgctct gcgggcactg
     2521 ctggagttgg gggcgaagga ggtctcggtg ctcgacgcgg agggccgcga gcagcggctg
     2581 ccgatcgagc agctcgcggt cggcgatcgc ttcctcgtcc gcccgggtga gaaggtcgcc
     2641 accgacggcg tggtcgagga gggccgctcg gcgatcgacc agtcgctgct gaccggcgag
     2701 tcggttccgg tcgaggttgc gcccggcgac gaggttgccg gggcgagcgt caacgccggc
     2761 gggcggctgg tcgtgcgcgc gacccgggtc ggcgccgaca ccgcgctggc ccagatcgcg
     2821 cggctggtca gcgacgcgca gaacggcaag gcgccggtgc agcggctcgc cgaccgcatc
     2881 tccggcgtct tcgtccccgc ggtgatcggc ctcgcggtcg ccacgctcgc cttctggctc
     2941 ggcgcggggg agggcgcctc cttcgccttc accgccgcgg tcgcggtgct gatcatcgcc
     3001 tgcccctgcg cgctcgggct ggcgacgccg accgcgctga tggtcgggac cggccgcggt
     3061 gcccagctcg gcctgctgat caagggcccc gaggtgctcg agtcgagccg tcgcgtcgac
     3121 acgatcgtgc tcgacaagac cggcaccgtc accaccggcc gcatggagct cgccgaggtc
     3181 gccgtcactc atgggggtga gggcaggcgt gaggcgctgc gcctggccgg ggcgctcgag
     3241 gacgcgtccg agcacccgat cgcgcgggcg atcgccgcgg cggctcgtgc cgaggggatc
     3301 gagctgccgc cggtcgagga cttccgaaac cgtgaggggc tcggggttga gggggtggtc
     3361 gacggccacg gggtccaggt cggccgcgcc tcgctgctgg ccgagtggag cctcgcgatc
     3421 cccgagcagc tcgccgccgc ccgtgcgcag gccgaggcgc gcggcgccac gccggtcttg
     3481 gtcgcctggg acggcgccgc ccgcgccgtg ctggttgtcg ccgacacggt caagccgacc
     3541 tcggccgagg cggtcgcggc gctgaagggt ctggggatcc ggccgctgct gctgaccggc
     3601 gacaacgagg cgaccgcacg cgcggtcgcc gccgaggtcg ggatcgatga ggtgatcgcc
     3661 gaggtgctgc ccgccgacaa ggccgcggtg gtcgcgcggc tgcaggccga gggacgcgtc
     3721 gtcgcgatgg tcggcgacgg ggtcaacgac gccccggcgc tcgcccgcgc cgacctcggg
     3781 ttggcgatcg ggaccggcac cgacgtcgcg atcgaggcct cggacctgac cctggtctcc
     3841 ggcgacctgc gcgccgccgc cgacgcgatc cggctctcgc ggtcgaccct gcgcacgatc
     3901 cgcgagaacc tcgcctgggc cttcggctac aacgtcgcgg ccctgccgct ggcggcggcg
     3961 ggcctgctca acccggtgat cgccggtgcc gcgatggcgc tctccagcgt ctccgtggtc
     4021 gccaactcgt tgcggctgcg ccgcttccgt aaagtcgggg gcgatgagtg aggagaccca
     4081 cggcgccgtc gacgccttcg tcgtcgagac gatcgccgcc gacgacgagg cgttgagcgc
     4141 ggcgctcgcc gacgccaccg ccgccggact gccggagatc gcggtgacgc cgccccaggg
     4201 gcgcctgctg accctgctgg cgcgctcggt cggcgcccgc acgatcctcg agttcggcac
     4261 cctcggcggc tacagcacga tccacctggc gcgggcgctg cccgacgacg ggcggctgat
     4321 ctcgctggag gcgaagccgg agttcgctga ggtggcgcgg gccagcatcg agcgcgccgg
     4381 gcttggcgac aaggtcgaga tccgggtcgg gccggcgctg gagagcctgc cggcgctgga
     4441 cgaggagggc gttggaccgt tcgacttcac cttcatcgac gccgacaagg tgaacacgcc
     4501 tcactacttc gcctgggcgc tggagaactc gcggcccggc tcgctgatcc tcgccgacaa
     4561 cgtcgtccgg ggcggcacgc tggccgacgc cgaggacccc gatcccgcta ccaaagcgca
     4621 acgcagcttc cacgaaacgc tcgccgagga gccccgcgtc ttcgccacga ccatccagac
     4681 cgtcggggcc aaggggcatg atgggttcac gctcgcgctg gtcgagtcgt gacagggctg
     4741 ctctaggcac gcccaaagac tctgtcctgc tatctttctc cgtcggccca gtgggccgtt
     4801 tggttgtgcc tgccgtccga ttcatggggc gcgcgggagt cctgttttcg attcagagac
     4861 cggagcaatg aaggttcgag cttccgtaaa gccgatgtgc gagaagtgca agatcatccg
     4921 ccgcaatggg gcggtcctag tgatctgcca aaacccccgt cataagcaga ggcagggcta
     4981 aggcatggca cgcatctccg gcgtcaacct cccggtcaac aaacgggtcg aaatcgggct
     5041 cacctacatc tttggggtcg gtcagtcgac ggccaaacag atcctcgctg agaccaaaat
     5101 cgatcccggc acccaggtca aggacctgac cgaggacgag gtcatcaagc tgcgcgacgc
     5161 ggtcgacaac cgcgaagtcg agggcgatct tcggcgcgag cgctcacaga acgtaaaacg
     5221 cctgatggag atcggctcct accgcggcct gcgtcatcgc cgtggcctgc cggtccgcgg
     5281 tcagcgcacg aagaccaacg cgcgcggtcg caagggcccg cggcggctga gcatcgccgg
     5341 taagcgcaag gtttagggga acacgaatgg ctgagaaaaa accctcgaaa gggcgttccc
     5401 gtcgtcgcgt caaaaagaac gtgacctacg ggcaggcgca catcaagact tctttcaaca
     5461 acacgatcgt caccctcacc gacaccgacg gcaacgtgat cgcctgggag tcggccggct
     5521 cggccgggtt caaaggctcg cgcaagtcga ccccgttcgc cgcccaggtg accgccgacg
     5581 cggccgccaa gaagggcatg gaacacgggc tcgagcgcgt tgaggtgttc gccaagggcg
     5641 ccggctcggg tcgcgagacg gcgatccgct cgctgcaggc cgccgggctc gaagtgacca
     5701 cggtcaagga cgtctccccg caagcccaca acggctgccg gccgaagaag cggaggcgcg
     5761 tctaaatggc tcgcgatacc gctccccagt gcaagcagtg ccggcgcgag ggccagaagc
     5821 tcttcctcaa gggtgagcgc tgcttcaccg acaagtgcgg cgtcgagcgt cgctcctacc
     5881 cgcccggtga gcacggccgc ggtcgtctgc gtcagtccga gtaccggctg cagctgcgcg
     5941 agaagcag
//
LOCUS       DASRIG010000364         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_709745, whole genome shotgun sequence.
ACCESSION   DASRIG010000364 DASRIG010000000
VERSION     DASRIG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709745"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFB52_09785"
     CDS             complement(<1..254)
                     /locus_tag="VFB52_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06668.1"
                     /translation="MSATWRRWVAWIGPHLDAIRNGDERALLGSLERFVTSHRFLAPF
                     AAALGGVAMLLAGLRLLIVNWRLTLVQIVPAVWIWVVMYDL"
     gene            376..1749
                     /locus_tag="VFB52_09790"
     CDS             376..1749
                     /locus_tag="VFB52_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZO06669.1"
                     /translation="MMRTVRPGVAFALLLIGFVLAPSAAYAVLGDFTEPASSPVPVGK
                     TGSSVAAGDFDNDGDNDLAVGYSAGVAVLTNNGKGDFSATGTSPETVQGGPASIVAGK
                     FNTDAHLDLVVGSETDEVDDGRVTVLLGDGKGDFTAGSPVSLKGAARDVAAADVDDDE
                     DLDVVVGEFFPAQVAILRNDGSGSLAAPLFEDAGGSGTNIFSIATGDFSGDGEEDIVV
                     GNPFTHEVSVYVNDGEEDGDFTEGTPVTGVDGYSLATGFFDAGGALDVAVATESAAAV
                     ILLGNGSGGLSKAASSPEALPGPSHAIASADFNADGKTDLAAVGNNTPGYAAILLGAG
                     DGDFAPAPSSPESVGSWPTAIAAAPLDKNTSTDLAIANIFGDKANILLNDFSASPPVE
                     NPPQQPPVVTPPATVPPEQKVFKCPKNKQLKKGKCVLKKCPKGKKLKKNGRCVKKKKP
                     PARSRRG"
     gene            complement(1768..3297)
                     /locus_tag="VFB52_09795"
     CDS             complement(1768..3297)
                     /locus_tag="VFB52_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06670.1"
                     /translation="MIRGKRALLGPIAAIAILASLAYAGPAGAEQIAYSCGEDICLIN
                     PDNPSEATNLTETHASAGHEQFPVFSPDGKLIAFRGLFVSGGFETWNIYTLDPAEPAP
                     REATDVSQSDDRGADFDAPAWSPDGTRLAWSSTASSSANPVQTGIYAGSAAGTSLPIQ
                     IGAREGSFPTWSADGTTIAFGGAPESVYIGAANAPGASTYLPGSAGLEPVWSPDGKLI
                     ATHRWNSYPYKMRINRADGSGGYELTKPGDLGTAIDWSSDSTRIVYVGDEEPIDRVWV
                     APADGSSPGHPIPMPKGWIVPHNPSFNSDGTRVVFTARQNTGPGYEQILVAPADGSAE
                     AVAITSSAMQNEQPSWKPGPGSGPKPPAGGAPPATQQPVKLKLSLYKNPRLVGGGYQR
                     MLLAGVDCHAPGGHPTGWVAEFCAANASAYPQGSAPAAGFRPWAKPKAKPPLFAKGKV
                     RVPEGKTKPLVLKITAAGRKLLKPGKKIKLKVTLTTKRSGAKKATTVTKTVTLKVPAK
                     K"
     gene            3406..4032
                     /locus_tag="VFB52_09800"
     CDS             3406..4032
                     /locus_tag="VFB52_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06671.1"
                     /translation="MGLVIAIAVAAFAVGAGSKSSKDVVICAAKKGGDLTLADKGKCA
                     KGEKKLTIAKQGPAGAVGPQGAPGPSGAPPALEAPHLVGPAIVACAVNTGTFCVTETG
                     LCWNMGNAGGGLAPVSFRKDSDGFVHLEGAFTNFNPEGACGGGETRPVFYLPQGFRPV
                     GGTQRFTVANCTGSKSSLVMIATNGLVSAGSEITNCMDLSGIVFHGDA"
     gene            4183..4851
                     /locus_tag="VFB52_09805"
     CDS             4183..4851
                     /locus_tag="VFB52_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZO06672.1"
                     /translation="MTEPDRLLPLSAEELLTTTRGVRKRLDFSRPVEREVLRECVATA
                     LQAPSGSNRWALQFVIVTDPEIRRAFGEVYRECYETYRSLDGVYIGSIDKGSEARNEQ
                     QRRTTVSADHLGENFGKAPAIVAACAPGRTEDGAAIRKTTQLGSVLPGMWSFMLAARL
                     RGLGTSWTTVSLFDEAKTGEALRIPIEEVTIGAITPVAYTLGTDFKPALRPNPDEVIH
                     WDGW"
     gene            4841..5593
                     /locus_tag="VFB52_09810"
     CDS             4841..5593
                     /locus_tag="VFB52_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO06673.1"
                     /translation="MAGSGSPVRTERRGRTLVIVLDRPEARNAVNQKMADGIAAALEE
                     LDADRELAVGVLWGSGPGFSAGMDLREIQAGGRSSVDERGFAGIVRYSAEKPLIAAIE
                     GFALAGGLEIALACDLIVAGAGARLGVPEVQRSLVAIGGALLRLPRRVPWGVASELVL
                     TGEPIDADRGYEVGLVDRLAPTGAALDVAVEIATTIGRNGPAAVRASKRVLREQLDWP
                     EAEFWERQAEIAEPVMKSEDAMEGARAFVERR"
BASE COUNT          899 a         1872 c         2076 g          876 t
ORIGIN      
        1 aggtcgtaca tcaccaccca gatccacacc gccggcacga tctgcaccag agtcaggcgc
       61 cagttgacga tcagcagccg caggccggcg agcagcatcg cgactccgcc gagcgcggcg
      121 gcgaacgggg cgaggaatcg gtgcgaggtg acaaagcgct ccagggagcc gagcagcgcc
      181 cgctcgtcgc cattgcggat cgcgtccagg tgcggaccga tccatgccac ccacctgcgc
      241 caggtcgcgc tcacgctcag gacactaggc gggtgaagct ggggaagtac gtacggttcc
      301 tgccgtattt gcatcctcac cacgtggcta tacgctgcgg gccttgtcac agtggaccac
      361 gggggaggtc gtttgatgat gcgcacggtt cgtcccggcg ttgcattcgc gttgctgttg
      421 atcgggtttg tgttggcgcc gagcgcggcc tacgcggtcc ttggtgactt caccgaaccg
      481 gcgagctcgc cggtgccggt ggggaaaacc ggatcgtccg tggccgccgg tgacttcgac
      541 aacgacggcg acaatgacct cgcggtcggt tacagcgccg gcgtggcggt gctgaccaac
      601 aacggcaaag gcgacttcag tgcgacgggg acctcgccgg agacggtcca gggtgggccc
      661 gcgagcatcg tcgcgggcaa attcaacacc gacgcccacc tcgacctcgt ggtcggcagc
      721 gagaccgacg aggtcgacga tggccgagtg acggttctgc tcggcgacgg caaaggcgac
      781 ttcacggctg gctcgccggt ctctctcaaa ggcgccgccc gcgacgtcgc cgcggcagac
      841 gtggacgacg atgaagacct cgacgtcgtc gtcggcgaat tcttcccggc ccaggtcgcg
      901 atcctgcgca acgacggcag cggctccctc gccgcacccc tcttcgagga cgccggcggg
      961 agcggcacca acatcttctc gatcgcgacc ggcgacttca gtggcgacgg ggaagaagac
     1021 atcgtggtcg gcaacccgtt cacccacgaa gtttcggtct atgtgaacga cggcgaggaa
     1081 gacggagact tcaccgaggg gactccggtg accggcgtcg acgggtactc gctggcgact
     1141 ggcttcttcg acgcgggcgg cgccctcgac gtcgccgtcg ccacggagag cgccgccgcg
     1201 gtgatcctgc tcggcaacgg gagcggcggg ctcagcaaag cggcgagttc gccggaggcc
     1261 ctgcccggcc catcccacgc gatcgccagc gccgacttca acgccgacgg caaaaccgat
     1321 ctggccgcgg tcgggaacaa cacgcccggc tacgccgcga tcctgctcgg cgctggcgac
     1381 ggcgacttcg caccggcgcc gtcctcgccg gagtcggtcg gctcgtggcc aaccgcgatc
     1441 gccgccgcgc cgctagacaa aaacaccagc acggacctcg cgatcgccaa catcttcggc
     1501 gacaaagcga acatcctgct caacgacttc agcgcgagcc cgccggtcga aaacccgccg
     1561 cagcagcccc cggtcgtgac gccgccggcg accgtccccc cggagcagaa agtgttcaag
     1621 tgcccgaaga acaagcaact gaaaaagggc aagtgcgtgc tgaagaaatg cccgaaaggc
     1681 aagaagctga agaagaacgg gcgctgcgtg aaaaagaaga aaccgccggc gcgctcgcgt
     1741 cgcggctgac ccggttcggc gccagcgcta tttcttcgcc ggcaccttta gcgtcaccgt
     1801 cttggtcacg gtggtggctt ttttggcgcc gctgcgtttg gtggtcagcg tcaccttcag
     1861 cttgatcttc ttgccgggct tcagcagctt cctgccggcg gcggtgatct tcagtaccag
     1921 cggcttggtc ttgccttcgg ggacgcggac cttgcccttg gcgaacagcg gcggtttcgc
     1981 cttcggcttg gcccaggggc ggaaaccggc ggcgggggca ctcccctgcg ggtaggcgct
     2041 ggcgttggcg gcgcagaatt cggcgaccca accggtcggg tggccgcctg gggcgtgaca
     2101 gtcgacgccg gcgagcagca tgcgctggta gccgccgccg accagccgcg ggttcttgta
     2161 gagcgagagc ttcagcttca cgggctgctg ggtcgccggg ggtgcgccgc ccgcgggggg
     2221 tttggggccg ctgcccgggc ccggcttcca cgacggctgc tcgttctgca tcgccgagct
     2281 ggtgatcgcg accgcctcgg cgctgccgtc ggcgggggcg acgaggatct gctcgtagcc
     2341 ggggccggtg ttctggcggg cggtgaaaac gacgcgggtg ccgtcggagt tgaagctggg
     2401 gttgtggggg acgatccagc ctttcggcat cgggatcggg tgcccggggc tggagccgtc
     2461 ggccggggcc acccagaccc ggtcgatcgg ttcttcgtca ccgacgtaga cgatcctggt
     2521 cgagtccgac gaccagtcga tcgcggtgcc gaggtcgccg ggcttggtca gttcgtatcc
     2581 gcccgagccg tcggcccggt tgatccgcat cttgtagggg taggagttcc agcggtgggt
     2641 ggcgatcagt ttgccgtccg gcgaccagac cggttcgagg ccggcgctgc cggggagata
     2701 ggtggatgcc cccggggcgt tggcggcccc gatgtagacg ctttccgggg cgccgccgaa
     2761 ggcgatcgtc gtcccgtcgg ccgaccaggt cgggaagctc ccttccctcg cgccgatctg
     2821 gatcggcagc gacgtgcccg cggcggaacc ggcatagatg ccggtctgca ccgggttggc
     2881 gctcgaactg gcggttgagg accaggccag tcgggtgccg tccggcgacc acgcgggagc
     2941 gtcgaagtcg gcgccgcggt cgtcggactg ggagacgtcg gtcgcttcgc gcggcgccgg
     3001 ttcggcgggg tcgagcgtgt agatgttcca ggtctcgaag ccgcccgaga cgaagaggcc
     3061 gcggaaggcg atcagtttgc cgtcgggcga gaagaccggg aactgctcat gtcccgcgga
     3121 ggcgtgggtt tcggtcaggt tcgtggcttc ggacgggttg tccgggttga tcaggcagat
     3181 gtcttcgccg cagctgtagg cgatctgctc ggccccggcc ggacccgcgt aggcgagcga
     3241 ggcgaggatc gcgatcgccg cgatcggccc cagcagcgct cgtttccccc tgatcacccg
     3301 cagcacctta aggaactaac acgaaggttt ccagtcgcgg ttgccttgac aggcacgggc
     3361 atgggtcgtt acaacccatc gatgcgtttg gggaagatcg cgtttatggg gctcgtcatc
     3421 gcgatcgcgg tggccgcctt cgcggtcggg gcggggtcga agtcctcgaa ggacgtcgtc
     3481 atctgcgcgg ccaagaaagg cggcgacctg acgcttgccg acaagggcaa gtgcgccaaa
     3541 ggcgagaaga agctgacgat cgcgaagcag ggaccggcag gtgcagtcgg tccgcagggc
     3601 gcccccggac cctcgggggc accgccggcg ctcgaagcgc cgcacctcgt ggggccggcg
     3661 atcgttgcct gtgcggtcaa caccggaact ttctgcgtca ccgaaaccgg gctctgctgg
     3721 aacatgggca acgccggcgg cgggctggcc ccggtctcct tccgcaagga ctcggacggc
     3781 tttgtgcatc tggaaggcgc cttcaccaac ttcaatccgg aaggagcctg cggaggcggc
     3841 gaaacgcgac cggtcttcta tctgccgcag ggcttccggc cggtgggcgg gacccagcgg
     3901 ttcacggtgg cgaactgcac gggctccaaa agcagcctgg tcatgatcgc gaccaacggc
     3961 ctcgtcagcg ccgggtcgga aatcaccaac tgcatggatc tgagcggcat cgtcttccac
     4021 ggcgacgctt agcgatcgcg tggggccatc catccaggct ggcgcccgat cccggcctgg
     4081 aacggacaac cctcggccgc ctaggcctgc ccgcttagcg gtttcacaaa ggcgctcgaa
     4141 tctcggcgcc cggtgggcgg ggatcggttc tacgttggcc tggtgacgga gcccgatcgg
     4201 ctgttgccgc tgagcgcgga ggagctgctc accacgacca ggggtgtgcg caagcggctg
     4261 gacttctcgc gcccggtcga gcgcgaggtc ctgcgggaat gcgtggcgac cgcgctgcag
     4321 gcgccctcgg gctcgaaccg ctgggcgctg cagttcgtga ttgtcaccga cccggagatc
     4381 cggcgcgcct tcggcgaggt ttaccgcgag tgttacgaga cctaccggag cctcgacggc
     4441 gtctacatcg gcagcatcga caagggcagc gaggcgcgca acgagcagca gcggcggacg
     4501 accgtctccg ccgaccacct cggcgagaac ttcggcaagg cgccggcgat cgtcgccgcc
     4561 tgcgcgcccg ggcgcaccga ggacggcgcg gcgatccgca agacgacgca gctcggcagc
     4621 gtgctgccgg ggatgtggtc gttcatgctg gcggcgcggc tgcgcgggct cggcacctcg
     4681 tggaccacgg tcagcctgtt cgacgaagcg aagacggggg aggcgctgcg gatcccgatc
     4741 gaggaggtga cgatcggggc gatcacgccg gtggcctaca ccctgggcac ggacttcaaa
     4801 ccggcgctgc gcccgaaccc cgacgaagtc atccactggg atggctggta gcgggtcgcc
     4861 cgtacgcacc gaacggcgcg gccggacgct ggtcatcgtc ctcgaccggc ccgaggcgcg
     4921 gaacgccgtc aaccagaaga tggccgacgg gatcgctgcg gccctggagg agctagacgc
     4981 cgatcgcgag ctggccgtcg gcgtgctctg gggctccggg cccgggttca gcgccgggat
     5041 ggacctgcgc gagatccaag cgggcgggcg ctcctcggtc gacgagcgcg gcttcgccgg
     5101 gatcgtccgc tactcggccg agaagccgct gatcgccgcg atcgagggct tcgccctggc
     5161 cggcggcctc gagatcgcgc tcgcctgcga cctgatcgtc gccggggccg gggcgcggct
     5221 cggcgtcccc gaggtccagc gctccctggt cgcgatcggc ggcgccctgt tgcgcctgcc
     5281 gcgccgggtg ccctgggggg tggcgagcga gctggtgctg accggcgagc cgatcgacgc
     5341 cgaccgcggc tacgaggtcg gtttggtgga ccgtctggcc ccgaccggcg cggcgctcga
     5401 cgtcgcggtc gaaatcgcga cgacgatcgg tcgcaacggc ccggccgcgg tgcgggcgag
     5461 caaacgggtg ctgcgcgagc agctcgactg gcccgaggcg gagttctggg agcgccaggc
     5521 ggagatcgcc gagccggtca tgaagtccga ggacgcgatg gagggagcgc gcgccttcgt
     5581 cgagcgccgc tagccgcgct ggcgcgggta gagcttctga gttcgaacac acaccctggg
     5641 aacaaacaac gaggagaggc acatgtacaa gctgttcgca tactggtcgg cgccgaagcc
     5701 cgaggacgtc gaggccttcg agg
//
LOCUS       DASRIG010000365         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1994762, whole genome shotgun sequence.
ACCESSION   DASRIG010000365 DASRIG010000000
VERSION     DASRIG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994762"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFB52_09815"
     CDS             <1..320
                     /locus_tag="VFB52_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO06674.1"
                     /translation="NVSILKPIHSEPVLESISKTGVAVTAEEHRVTGGLADAVRHVAA
                     EQHPVPIFAVGMGDEFSVSGTGEACMEHFGLTARGIADRAREALVLKPIVSRPPLFGP
                     EWT"
     gene            386..769
                     /locus_tag="VFB52_09820"
     CDS             386..769
                     /locus_tag="VFB52_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06675.1"
                     /translation="MRRAVAATATLALVLSAATWAGCGDDDDETTTTTGSAASSAEQK
                     VDAAVKSCSDEAQQLGGAAGTALESACTSVGASVKQALSSGGEDVKQALSKAASSCRS
                     AVQQLPSGQAQDALTKLCDSVASAE"
     gene            838..2268
                     /locus_tag="VFB52_09825"
     CDS             838..2268
                     /locus_tag="VFB52_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZO06676.1"
                     /translation="MATAAPREAADRAADLTPTERAAAGKAARQRVPRSSHGKWEPAT
                     DRPDPVEVLERQAAERVPELVPLRYGRMLASPFTFYRGAAAIMAADLAATPSSGLWVQ
                     ACGDAHLSNFGGFASPARGMVVDVNDFDETLPGPWEWDVKRLAASFEIGARDRGFDDA
                     ERTAIARTSARAYREHMRELAELSNLDVWYRRVDIAQIRKAVAPMVSKEERKRFDRTV
                     AKAQRKTRMRAFSKLTRRVDGELRMASDPPVLVPFEELFSGTQLSTAEAQVRRLVEEY
                     GATLSAEYRRLFDSYRFVHAARKVVGVGSVGTRAWVALFVGRDEDDPLFLQIKEAQPS
                     VLEPYTAPTEFPHQGQRVVVGQRLTQATGDILLGWLTATGPDSKKRDFYVRQLWDEKG
                     SAIVELMSPRTMGIYAQVCGGILARAHARSGDRIAIAGYLGGGDTFDRAIPEFAAAYA
                     DQNEDDYRALKRAADDGRIAVESEGQ"
     gene            2333..3892
                     /locus_tag="VFB52_09830"
     CDS             2333..3892
                     /locus_tag="VFB52_09830"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZO06677.1"
                     /translation="MHRREALTALLIALVVALALAPAAVAKPDQQIGARSLGDPLLPQ
                     LGNGGYDVDHYRISLDYDPAANELTSAKTRIVATATQTLREFSLDFQDVLEVSSVKVG
                     GRSADFSQVEATPDLSDDPVVTQPMKLVVEPPPSTRPKPGREFTVKVSYSGTPEEITD
                     PDTSIEGWIRACYPLDPPQTCDGAFVVNEPMGAQSWFPSNNYPTDKATFDTLIRVPQS
                     KTALGVGELAGRTEHGDGTATWHWSEDDPTATYLTTATVGDFIYREESMVETSTGRTL
                     PVYNAIDSTATQTQLDAINASLAEAPAQVNFLSDIYGPYPFDSTGAVADRAAGVGYAL
                     EVQTKPHYSGGFVSGNPSISISTQLHELAHQWFGNSATLETWADIWFNEGWANWSDWY
                     WSFLEDGGDDPAEIFDGLYADTPPEDWETAPAILDGDPANLFHFFPTYQRGAMTLQGY
                     REIVGDDTFFELARTLFDDFGHGNVSTEEFIETAKEVSGLTGADLALLDDYFQQWLYG
                     TERPTIVPEDF"
     gene            complement(3926..>5095)
                     /locus_tag="VFB52_09835"
     CDS             complement(3926..>5095)
                     /locus_tag="VFB52_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06678.1"
                     /translation="VYALVGASGAIAAAIGPLLGGFLTTLLSWRVGFMLEAVIIAGVL
                     AGSGLIRDVPYTGDRGVDVVGAVLSVLGMGCLVLGILAWQEGGDSVAALLAVGLAGIA
                     GLAWWLVRRKRDGKPALIDPELFKIKLFRFGITEGVLQQIALGGLLIALPIYLQLDLG
                     YNALQAGLTLAPLSLTIFGVALLAGRRAGRRRPSGLIRAGFGLLAGGVLLLIAVVPRA
                     DSGWHLVPALLLAGGGMGLLVSQINNYTLSPIPEERAGEASGVNSAVSSFGLSVGLAF
                     AGAILLAALSILFTNKSEASTVLSPTEQEQVANVLEDDAQVMSDAQLEELLAGQPAPT
                     QEEILRINDEARPEALRFALLVPLVAAVIGLFASFPMMRLPDPVPSEAAEGAALG"
BASE COUNT          878 a         1791 c         1734 g          692 t
ORIGIN      
        1 tgaatgtctc gatcctgaag ccgatccact ccgagccggt cctcgagtcg atctcgaaga
       61 ccggcgtggc tgtgacggcc gaggagcacc gggtgaccgg cgggctagcc gacgcggtcc
      121 gccacgtggc ggccgagcag cacccggtgc cgatcttcgc ggtcggaatg ggcgacgagt
      181 tcagcgtgtc gggaaccggc gaagcctgca tggagcactt cggcctcaca gcgcggggga
      241 tcgccgaccg cgcacgggag gcgctggtgc tgaagccgat cgtctcgcgc ccgccgcttt
      301 tcggccccga gtggacctga gcggaccggc acgacgggag tgggggttga cgaggggcac
      361 ctgcgcgtga tctgatagcg ccgagatgcg acgcgccgta gccgccaccg cgactcttgc
      421 cctcgtccta tccgctgcga cctgggcggg gtgcggcgac gacgacgacg agaccacgac
      481 cacgactgga tccgccgcat cgagcgccga gcagaaagtc gacgcggcgg tcaaatcgtg
      541 cagcgacgag gcccagcaac tcggcggcgc ggcggggacc gcactcgagt cggcctgtac
      601 gtccgtcggc gcgtccgtca agcaggcgct gagcagcggc ggcgaggacg tcaagcaggc
      661 gctgtcgaag gcggcgagct catgccggag tgcggttcag cagctcccct ctggccaggc
      721 gcaggatgcg ctgacgaagc tctgcgattc ggtcgcaagc gcggagtagg cgtcgtttga
      781 cccgagcggc gttcagcgac gagcgcgagc tcgagatcga gctcaagtgg tgagtcgatg
      841 gccacagcag caccaaggga ggctgccgat cgggccgcag atctgacccc gaccgagcgg
      901 gcagcggcgg gcaaggcggc gcgccaacgg gtgccgcgct ccagccacgg caagtgggag
      961 ccggcgaccg accggcccga cccggtcgag gtgctcgaac gccaggctgc ggagcgtgtt
     1021 cccgagctcg ttcccttgcg ctacggccgc atgctcgcct cgccgttcac gttctaccgc
     1081 ggcgccgcgg cgatcatggc cgcggacctg gcggcaacgc ccagctccgg cctatgggtt
     1141 caggcgtgcg gcgacgcgca cctctcgaac ttcggcgggt tcgcttcgcc ggcccgaggc
     1201 atggtcgtgg acgtcaacga cttcgacgag accctcccag ggccgtggga atgggacgtc
     1261 aagcggctcg cggccagctt cgagatcggc gcccgcgacc gcggcttcga cgacgccgag
     1321 cgaaccgcga tcgcgcgcac ctcggcgcgg gcttatcgcg agcacatgcg cgagctggcg
     1381 gagctcagca atctcgacgt ctggtaccgc cgcgtggaca tcgcgcagat tcgcaaggcc
     1441 gtcgcgccga tggtcagcaa ggaggagcgc aagcgattcg accgcaccgt ggccaaggca
     1501 caacgcaaga cccggatgcg cgccttctcg aagctaaccc gccgcgtcga cggcgagctc
     1561 cggatggcca gcgacccacc ggtcctggtg ccgttcgagg agctgttctc gggcacgcag
     1621 ctgagcaccg ccgaggcgca ggtacgaagg ctcgtcgagg agtacggagc gacgctcagc
     1681 gccgagtacc gccggctgtt cgacagctac cgcttcgttc acgccgcgcg caaggtggtc
     1741 ggcgtcggca gcgtcggcac ccgcgcctgg gtagccctct tcgtcggtcg cgacgaggac
     1801 gacccgctgt tcctgcagat caaggaggcg cagccctcgg tgctcgagcc ctacacggcg
     1861 ccgaccgagt tcccccacca gggccagcgg gtggtggtgg gacagcggct gacccaggcc
     1921 acgggcgaca tcctgctcgg ctggctgacc gcgaccgggc cggactcgaa gaagcgcgac
     1981 ttctacgtgc gtcagctctg ggacgagaag ggctcggcga tcgtcgagtt gatgtcaccc
     2041 aggacgatgg gcatctacgc ccaggtctgc ggcggcatcc tcgcgcgcgc ccacgcgcgg
     2101 tcgggcgacc ggatcgcgat cgccggctac ctcggcggcg gcgacacctt cgatcgcgcg
     2161 atccccgagt tcgccgccgc atacgccgac cagaacgagg atgactacag ggcgttgaaa
     2221 cgggcggccg acgacggccg catcgcggtc gaatcggaag gccaatgaca gacgatggta
     2281 agaacacagt gatgcgagtt tgagcgcttc cccgctcgga tacatgaagc gcatgcatcg
     2341 cagggaagcg ctgacggcac tcctaattgc cctcgtcgtc gccttggcct tggcgcccgc
     2401 cgcggtcgca aagcccgacc agcagatcgg cgcccgctcg ctcggcgacc cgctgctgcc
     2461 gcagctcggc aacggcggct acgacgtcga ccactaccgg atcagcctcg actacgaccc
     2521 cgcggcgaac gagctcacgt cggcgaagac caggatcgtc gcaacggcga cgcagacgct
     2581 gagggagttc agcctcgact tccaggacgt gctcgaggtt tcctcggtca aggtcggcgg
     2641 gcggtcggcc gacttcagcc aggtcgaggc gaccccggac ctgagcgacg accccgtcgt
     2701 cacccagccg atgaagctcg tcgtcgagcc gcctccctcg acgcggccga agccgggccg
     2761 cgagttcacg gtcaaggtct cctacagcgg cacgcccgag gagatcaccg accccgacac
     2821 ctcgatcgag ggctggatcc gcgcctgcta tccgctcgac ccgccgcaga cctgcgatgg
     2881 cgccttcgtg gtcaacgagc cgatgggcgc ccagagctgg ttcccgtcga acaactatcc
     2941 gaccgacaag gcgaccttcg acaccctgat ccgcgtgccg cagtcgaaga ccgcgctcgg
     3001 cgtcggcgag ctcgccggac ggaccgagca cggcgacggg actgcgacct ggcactggag
     3061 cgaggacgat ccgaccgcga cctacctgac cacggccacc gtcggtgact tcatctaccg
     3121 cgaagagtcg atggtcgaga cctcgaccgg ccggacgctg ccggtttaca acgccatcga
     3181 cagcacggcc acgcagacgc agctcgacgc gatcaatgcc tcgctcgccg aggccccggc
     3241 tcaggtcaac ttcctctcgg acatctacgg cccgtacccg ttcgactcca ccggcgcggt
     3301 cgccgatcgc gccgccggcg tcggctacgc gctcgaggtg cagaccaagc cgcattattc
     3361 gggcggtttc gtcagcggca acccctcgat cagcatcagc acccagcttc acgagctcgc
     3421 ccaccagtgg ttcggcaaca gcgccacgct cgagacctgg gccgatatct ggttcaacga
     3481 gggctgggcc aactggtcgg actggtactg gagcttcctc gaggacgggg gcgacgaccc
     3541 ggcggagatc ttcgacggcc tctacgccga caccccgccg gaggactggg agacagcccc
     3601 ggcgatcctc gacggcgacc ccgcgaacct gttccacttc ttcccgacct accagcgcgg
     3661 tgcgatgacc ctccagggct accgcgagat cgtcggcgac gacaccttct tcgagctcgc
     3721 gcggacgctg ttcgacgact tcggacacgg caacgtcagc accgaggagt tcatcgagac
     3781 ggcgaaggag gtcagcggcc tgaccggcgc cgatctcgcg ctgctcgacg actacttcca
     3841 gcagtggctg tacgggaccg agcggccgac gatcgtgccc gaggacttct agccgtccga
     3901 ccgatcccgt cgccgacgta tgcggtcagc ccaatgccgc gccctcggcg gcctcggacg
     3961 gcaccggatc cggcaggcgc atcatcggga acgaggcgaa cagcccgatc acggcagcca
     4021 cgagcgggac gagcagcgcg aaccgcagcg cctcgggcct ggcctcgtcg ttgatgcgga
     4081 ggatctcctc ctgcgtgggc gcgggctgac ccgcgagcag ctcctcgagc tgggcatcgc
     4141 tcatcacctg cgcgtcgtcc tccaacacgt tggcgacctg ctcctgctcg gtggggctca
     4201 gcacggtgct cgcctcggat ttgttggtga agaggatgga gagcgcggcg agcaggatcg
     4261 cgccggcaaa ggccaatccg acggagagtc cgaaggagct caccgccgag ttgacgccgg
     4321 aggcctcccc cgcccgctcc tccgggatcg gagagagcgt gtagttgttg atctgggaga
     4381 ccagcagtcc catcccccct ccggcaagca atagtgcggg cacgaggtgc cagccgctgt
     4441 ccgcccgtgg cacgacagcg atcaggagca gtaccccgcc ggcgagcagc ccgaaccccg
     4501 ctcggatgag gccgctcggg cgcctcctcc ccgctcgtct ccccgcgagc agcgcgacgc
     4561 cgaagatcgt gagcgacagc ggcgcgagcg tgaggcccgc ctgcagcgcg ttgtagccga
     4621 gatccagctg caggtagatg ggcagcgcga tcagcaggcc gccgagggcg atctgctgga
     4681 gcactccctc ggtgatcccg aagcggaaca gcttgatctt gaacagctcc ggatcgatca
     4741 gtgccggctt gccgtcgcgc ttgcggcgaa cgagccacca tgcgaggccg gcgatgcccg
     4801 ccaggcccac ggccaatagc gccgccaccg agtcgccgcc ctcctgccag gccaggatgc
     4861 cgagcacgag acagcccatg ccgaggacgg aaaggaccgc gccgacgacg tcgacgcctc
     4921 gatcgccggt gtagggcacg tcgcgaatga ggcccgagcc ggccagcacc cccgcgatga
     4981 tcacggcctc gagcatgaac ccgacccgcc aggagagaag ggtggtgaga aacccgccga
     5041 gcagcggccc gatcgctgcc gcgatcgcac ccgaggcccc gacgagggcg tagac
//
LOCUS       DASRIG010000366         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3283380, whole genome shotgun sequence.
ACCESSION   DASRIG010000366 DASRIG010000000
VERSION     DASRIG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283380"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFB52_09840"
     CDS             complement(<1..360)
                     /locus_tag="VFB52_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZO06679.1"
                     /translation="MATAGPLPSDIQPVENETRDRIITGLVTLIPFVAIGLAGWQVWG
                     SALHWSDVFLFLGMYFFTGLGITVGFHRLFTHRSFKTSNWMRGLLAILGSAAIEGPII
                     SWVADHRKHHAFSDQEGD"
     gene            439..1653
                     /locus_tag="VFB52_09845"
     CDS             439..1653
                     /locus_tag="VFB52_09845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZO06680.1"
                     /translation="MPFANPAPLRREIEARLPDRPFTVEFWDGSRLPATSEGGPTFTV
                     RSPRAAAHALRAPGQLGLGRAYVSGELDVDDMDATIKLLNEWQPPSLDGADKRALMLG
                     AVRAMGLTPPPPRPDAELRPSGKRHSKERDARAVRHHYDVSNEFFELFLGPTMVYSCA
                     IWNKGAKTLEEAQEEKLATVARKLELKEGDRVLDVGCGWGGFPLHAATKHGCTVVGIT
                     LSPPQAEKARERAEAARVGDKVDIRVMDYRDIVGTGEKFDAIASIGMVEHVGSANIDV
                     YAQTLAAVLEPGGRLLNHGITRLRHTDAEAGDFSERYVFPDAAPLHLSRNLLALERAG
                     FVTHHVEDFAPDYAETLKHWYENLDANLERATELAGEERIRVWRLYLRAARNGFLSGF
                     TSIYQARCELVN"
     gene            complement(1650..2321)
                     /locus_tag="VFB52_09850"
     CDS             complement(1650..2321)
                     /locus_tag="VFB52_09850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZO06681.1"
                     /translation="MTAIKAVLWDIDGTLLKTGGAGAVAWQRAFRELWEVDANITEHT
                     REGMTDPEIVEIIFREVIGRDGSDAEHAEAIAGYLSHLQDAVDESPGYELMPGITEIL
                     PRLAASGVLQGLITGNIEAAAHIKLSRGDLNQYFAFGGYGSDARDRTELTKRGVERGG
                     EVAGHPLDLAATIGVGDTPRDVSACHGAGIRVVGVATGTYSVEEQRDAGADWALATVE
                     DGFPV"
     gene            complement(2351..3580)
                     /locus_tag="VFB52_09855"
     CDS             complement(2351..3580)
                     /locus_tag="VFB52_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZO06682.1"
                     /translation="MKKMPAPAKLLASGATVATALVLVSLVLPALALAAPQEGPGTVT
                     IAPDPLVVPATTVGNQGEWLAVDVSYEGEGEAAIDKVSLLGEGAGEFSSNGSDCGILT
                     SGQHCTAWFALKPTTLGTKEATLEVRFQGERPAELRPISGRSVPPQLSFSPASHDFGI
                     QRVNRDSVGNSFQLTNTGEAPVSPNNFEISGDSDVFWTGSSDCWTLLAPGASCSMQVW
                     FGPREVREYAAQLRVWANGEAFTAALSGRGGQAIVGPSTNPVDFGSAAVDSGGVVKTI
                     TLSNSGDLGAAYFIAVIAGGDAGSFQLLSESCTMVALMPTDSCSVRVRFDPDEPGPKS
                     ARFAMFGDGDDGVMVFLDGEGLAAAPAEAGPASGESAVASSRKGRRKRFGRNKGIHAP
                     SAKAARRSELRAGAARR"
     gene            complement(3656..>4466)
                     /locus_tag="VFB52_09860"
     CDS             complement(3656..>4466)
                     /locus_tag="VFB52_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZO06683.1"
                     /translation="IFILVVLWTAFPPAIKAIFSTLYVPISLAALGIVLRGAGFAFGK
                     SIEGPRERRLAGATFALSSVLTPFFMGTVVGAIAAGHVPADGNGEAFSSWIQPLPLLV
                     GGMFVAAGAYLSAVFLVGDARRAGEADMEAYFVRRALGAAAVAGLAAAVGLIELHAEA
                     RYVFDRLVDQGLPLVILSTICGIGLLAALLRGWRRPIRPLAAGAVIAVIWGWAVAQFP
                     YLLPTSLRIDQAAAPDPTLTVIFIVFAIAAVLVLPSLFLLYTLSQRDLLEH"
BASE COUNT          700 a         1543 c         1610 g          613 t
ORIGIN      
        1 gtcgccctcc tggtcggaga aggcgtggtg cttgcggtgg tcggcgaccc aggagatgat
       61 cgggccctcg atcgccgccg agccgaggat cgccagcagc ccgcgcatcc agttggaggt
      121 cttgaacgag cggtgcgtga acaggcggtg gaagccgacc gtgatcccca gcccggtgaa
      181 gaagtacatg ccgaggaaga ggaacacgtc gctccagtgc agggcacttc cccagacctg
      241 ccaaccggcg aggccgatcg ctacgaacgg gatcagcgtg accaggccgg tgatgatccg
      301 gtcgcgggtc tcgttctcga ccggctgtat gtctgagggg agaggtcctg cagtcgccat
      361 gcgcggcgat catacgggtc agagctgaag ccagcattag ggtcccctga tagcgccccg
      421 tcgataggct ccagagcggt gccgttcgcc aaccccgccc ccctgcgccg cgagatcgaa
      481 gctcgccttc cagaccgccc gttcacggtt gagttctggg acggatcgag gctgccggcg
      541 acctccgagg gcggcccgac cttcaccgtg cgctcgccgc gcgccgccgc ccacgcgctg
      601 cgcgcgcccg gccagctcgg cctcggccgc gcctacgtca gcggcgagct cgacgtcgac
      661 gacatggacg cgacgatcaa gctgctgaac gagtggcagc cgccctcgct cgacggcgcc
      721 gacaaacggg cgctgatgct gggcgccgtg cgcgcgatgg gcctgacccc gccgccgccc
      781 cgccccgacg ccgagctgcg cccgagcggc aagcgccaca gcaaagagcg cgacgcccgc
      841 gccgtccgcc accactacga cgtctccaac gagttcttcg agctcttcct cggaccgacg
      901 atggtctaca gctgcgcgat ctggaacaaa ggcgcgaaaa cgctggagga ggcccaggag
      961 gagaagctgg cgaccgtcgc ccgcaagctc gagctgaagg agggcgaccg ggtgctcgac
     1021 gtcggctgcg gctggggcgg tttcccgctg cacgcggcga ccaaacacgg ctgcaccgtg
     1081 gtcgggatca ccctctcgcc gccgcaagcc gaaaaagcgc gcgaacgggc cgaggcggcc
     1141 cgggtcggcg acaaggtcga catccgggtg atggactacc gcgacatcgt cggcaccggt
     1201 gagaagttcg acgcgatcgc cagcatcggc atggtcgagc acgtcggctc ggccaacatc
     1261 gacgtctacg cgcagaccct ggccgcggtg ctggagccgg gcgggcgcct gctcaaccac
     1321 gggatcaccc gcctgcgcca caccgacgcc gaggccggcg acttctccga gcgctacgtc
     1381 ttcccggatg ccgccccgct gcacctctcg cgcaacctgc tggcgctcga acgagccggg
     1441 ttcgtcaccc accacgtcga ggacttcgcc cccgactacg cggagacgct gaagcactgg
     1501 tacgagaacc tcgacgcgaa cctggagcgg gcgaccgagc tggccgggga ggaacggatc
     1561 cgcgtctggc gcctctacct gcgcgccgcc cgcaacggct tcctcagcgg cttcaccagc
     1621 atctaccagg cccgctgcga gctggtcaac tagaccggga agccgtcctc aaccgtcgcg
     1681 agcgcccagt cggcgccggc gtcgcgctgc tcctcgacgc tgtaggtgcc ggtggcgacg
     1741 ccgaccacgc ggatgccggc gccgtggcag gcgctgacgt cgcgcggggt gtcgccgacg
     1801 ccgatcgtgg cggcgaggtc gagcgggtgg ccggcgacct ctcccccgcg ctcgacgccg
     1861 cgcttggtca gctcggtgcg gtcgcgggcg tcggagccgt agccgccgaa ggcgaagtac
     1921 tggttgaggt cgccgcggct gagcttgatg tgggcggcgg cctcgatgtt gccggtgatc
     1981 aggccctgga ggacgccgga tgcggccagg cgcggcagga tttccgtgat ccccggcatc
     2041 agttcgtagc cgggcgactc gtcgaccgcg tcctgcaggt ggctgaggta gccggcgatc
     2101 gcttccgcgt gctcggcgtc gctgccgtcg cggccgatca cctcgcggaa gatgatctcg
     2161 acgatctcgg gatcggtcat cccttcgcgc gtgtgttcgg tgatgttggc gtcgacctcc
     2221 cagagctcgc ggaaggcgcg ctgccaggcg accgcccccg ccccgccggt cttcagcagg
     2281 gtgccgtcga tgtcccagag aaccgccttg atcgccgtca ccggccgcaa cctagagatc
     2341 tgcccggcgg ctaacgccgg gcggcgccgg cgcgcagctc ggaccggcgg gccgccttgg
     2401 cgctcggggc gtggatcccc ttgttgcggc cgaagcgctt gcggcggccc ttgcgcgacg
     2461 aggcgaccgc gctctcgccg ctcgccggtc cggcctcggc cggcgcggcg gccagccctt
     2521 cgccgtcgag gaagaccatc acgccgtcgt cgccgtcgcc gaacatcgcg aaacgggcgc
     2581 tcttcgggcc cggttcgtcc gggtcgaagc ggacgcggac ggagcaggaa tcggtcggca
     2641 tcagcgcgac catcgtgcag ctctcgctca gcagctggaa gctgccggcg tcgccgcctg
     2701 cgatcacggc gatgaagtac gccgccccga ggtcgcccga gttggagagc gtgatcgtct
     2761 tgaccacacc gcccgagtcg acggcggcgg atccgaagtc cacggggttc gtgctcgggc
     2821 cgacgatcgc ttgtccgccg cggccgctga gcgcggcagt gaaagcttcg ccgttggccc
     2881 agacccgcag ttgcgccgcg tattcgcgca cctcgcgcgg gccgaaccag acctgcatcg
     2941 agcagctggc gccgggtgcg agcagcgtcc agcagtcgct gctgccggtc cagaacacat
     3001 cgctgtcgcc gctgatttcg aagttgttcg gactgaccgg cgcttcgccg gtgttggtga
     3061 gctggaagct gttgccgacc gagtcgcggt tgacgcgctg gatcccgaag tcatgagatg
     3121 ccgggctgaa gctgagctgc ggcggcaccg agcggccgct gatcgggcgc agctcggccg
     3181 gccgttcgcc ctggaagcgg acctcgagcg tcgcttcctt ggtcccgagc gtggtcggct
     3241 tcagcgcgaa ccaggcggtg cagtgctggc cgctggtcag aatgccgcag tcggagccgt
     3301 tggaggagaa ctcgccggcg ccttcgccga gcagcgagac cttgtcgatc gccgcttcgc
     3361 cttcgccttc gtaggagacg tcgaccgcca gccactcgcc ctggttaccg accgtggtcg
     3421 ccgggacgac cagcgggtcg ggcgcgatcg tgaccgtgcc ggggccttcc tggggagcgg
     3481 cgagggcgag cgccggcaga accagggaga cgaggacgag ggcggtggcg accgtggcgc
     3541 cggaggcgag gagcttcgcc ggtgcgggca tctttttcat gcgtccccat cgggccgtca
     3601 ccggcgcggc tcgcgaaatg gatgggaaat cccacctttt tgcagcgaaa atgccctagt
     3661 gctccaacag atcccgctgg ctgagcgtgt agagcaggaa cagcgagggc agcacgagca
     3721 cggcggcgat cgcgaagacg atgaagatca cggtcagcgt cgggtcgggg gcggcggcct
     3781 ggtcgatccg caaactggtc ggcagcaggt aggggaactg ggcgacggcc cagccccaga
     3841 tcaccgcgat caccgcgccc gcggcgagcg gccggatcgg ccggcgccag ccgcgcagga
     3901 gcgccgccag caggccgatc ccacagatcg tcgagaggat caccagcggc agcccctggt
     3961 cgacgaggcg gtcgaagacg taacgcgctt cggcgtgcag ctcgatcagg cccaccgcgg
     4021 cggcgaggcc ggcgacagcg gcggcgccga gcgcgcgacg cacgaagtag gcctccatgt
     4081 cggcttcgcc ggcgcggcgc gcgtcgccga ccaggaagac cgccgagagg taggcgccgg
     4141 cggcgacgaa catgccgccg acgagcagcg gtagcggctg gatccagctc gagaacgcct
     4201 cgccgttgcc gtcggcgggg acgtggccgg cggcgatcgc gccgaccacg gtgcccatga
     4261 agaacggggt cagaaccgag gagagggcga aggtcgcgcc ggccaagcgc cgctcgcgcg
     4321 gcccttcgat cgacttgccg aaggcgaagc cggcgccgcg caggacgatc cccagcgccg
     4381 ccagcgagat cggcacgtag agggtcgaga agatcgcctt gatggccggc gggaaggcgg
     4441 tccagaggac gacgaggatg aagatc
//
LOCUS       DASRIG010000367         5944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2251225, whole genome shotgun sequence.
ACCESSION   DASRIG010000367 DASRIG010000000
VERSION     DASRIG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251225"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFB52_09865"
     CDS             complement(<1..1115)
                     /locus_tag="VFB52_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06684.1"
                     /translation="MNEDRNAFQNVAALFAALWRLVRGEDQRGRKVKWLVSQLRPYRG
                     RVALMFVGLLGATAAGLAPPYLAGQAIDTGIIAGDVGALDRIVAAFLAVAVLYAAATY
                     LQTYMVGWVGTRVLQNLRQRVFEHIQGMSIGFFTRRSPGVLISRMTNDIEALNQLVTD
                     GVVTIFSSTLTLLGVVVILLVLDVQLALVVFLTFPLLLIASIVFRIVSHGAYRATRER
                     IAAITAYLQESLSGVRVVRSFGQEPRHLDEMTELNEANRAVNMKTVYLNASYFPAVEL
                     LAALATAVILVYGGGLAIDKEIQVGVIVAFVGYLTTFFEPIQQLSQLYTTYQQGMAAL
                     DKIFDLLDTEPDMVDAPDAIDPGTIRGEIELEGVWFS"
     gene            complement(1112..2902)
                     /locus_tag="VFB52_09870"
     CDS             complement(1112..2902)
                     /locus_tag="VFB52_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO06685.1"
                     /translation="MRTYRRLMGFLRPYRLQLWGSLVFAWAAMGMTVLIPWLIGRAVN
                     AIVEEEKPDLLPLALAILGAAILRLGLTVVRRLVAGKVSLAVEFDLRQKFYAHLQRLE
                     LGFFDGQQTGQLMSRATVDLGAIRFFLGYGLIFITQNLLTIALAGTVMFFIDPLLALL
                     ALAPAPLIVFTASRYNRVSRPAQQEVQQRIAELTAEAEENVSGIRIVKAFAREEHQLH
                     RFQRAVSRVFDQSIYSTKLQAFFSPLIGLLPQIGIALVLLVGGRLVIDDKMNIGELTA
                     FYTYLVMLAGPLRMLGVAMGMAQRAIASGNRLFEILDREPRIESAPGAPPLPAGGGRV
                     ELRGVTLRYAGAAEADEPAAPALRDVSLEVEAGRTVALVGPSGSGKTSLVALIARLYD
                     PDEGTVLVDGADVRTVDVPSLRSEVAFVADDSFLFTASVAENIAYAQPEATREQIEAA
                     ARRAQADAFIRALPDGYETRVGERGLTLSGGQRQRVAIARALLADPRVLILDDATSSV
                     DATTEVAIKEGLQEAMAGRTTFIIAHRLSTVSLADEIVVVDGGEIVDRGTHEELMEGC
                     GFYREIAEHGLADSVFLQRDLEEREEMARL"
     gene            complement(3336..3974)
                     /locus_tag="VFB52_09875"
     CDS             complement(3336..3974)
                     /locus_tag="VFB52_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06686.1"
                     /translation="MRLRTATIAALAAAVVAIALALIFGEGESKQAQAKTEAAPGTPS
                     IEIISPRNGARQSSHAVVVKVDIENFTLAPLQFGNEPQLGQGHVRYSLNRVPDCVERE
                     KLLRAVHSPVGSGRLIGASMDYPEFSGPNGVLAEELGTASSYSPGTRPEIYYHNLNPG
                     FYRLVVTLAQNSGAPTAHHAVTNFQVLPKPGHGPKPCPKGKIPSAKAAAQLR"
     gene            4052..4459
                     /locus_tag="VFB52_09880"
     CDS             4052..4459
                     /locus_tag="VFB52_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZO06687.1"
                     /translation="MYRIGAAARHPANWLQLFKFGLVGGSGYLINLAVFAFATGVLDI
                     HHLLAAVLAFVVAVSNNFWWNRHWTFAAGDGAVHFQAARFFTVSIAGLGINLAVLEAL
                     VASNTVGDLPAQAIAVAVAMPFNFVCNRLWTFA"
     gene            4490..>5944
                     /locus_tag="VFB52_09885"
     CDS             4490..>5944
                     /locus_tag="VFB52_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO06688.1"
                     /translation="MSGNKPTYDPARIEPQRQEAWEQRGDYSAPPRPEPGQTPIYVKA
                     SSPFTSGNLHMGHVRDYSLGDAYARFHRARGDAVLLGFGFDAFGLPAELAAIERQVLP
                     SDWVRQCGDRMLAQMKRLGYSFDYDRVFYSSDESFYRWSQWLFLTLLEMDMIYSEDAT
                     VDWCDTCQTTLATIQVEEGGTCWRCHNEVRLIRRPTWFLRITPYLEENDENTGKLANW
                     NELALATQHYILGRSEGVELELEGSGGKLTVFTPHREFAGHASFVLLSPRHPEVDAWI
                     ADDEVREQLEQMRSGGWERSARDASAVPLFDTGATLAGPGGGELPVLVSPLVDARFGP
                     TAALGIPLVDEADRDLAARAERVATFEAAEEGEAPAPAPVAAAGEAKRYRANDFSISR
                     QRSWGTPIPIIHCESCGVVPVPVEDLPVKLPRDLVPSGEGNPLAERPDFVDVACPKCG
                     EPAKRETDTLDCHFDALFLWVPAAVPPEARAEQMF"
BASE COUNT          919 a         2059 c         2059 g          907 t
ORIGIN      
        1 tagctgaacc agacgccctc gagctcgatc tccccgcgga tcgtgccggg gtcgatcgcg
       61 tccggcgcgt cgaccatgtc gggctcggtg tcgagcaggt cgaagatctt gtcgagcgcc
      121 gccatcccct gctggtaggt cgtgtagagc tgggagagct gctggatcgg ttcgaagaag
      181 gtggtcaggt agccgacgaa ggcgacgatc acgccgacct ggatctcttt gtcgatcgcc
      241 aggccaccgc cgtagacgag gatcaccgcg gtcgccagcg ccgccagcag ttcgaccgcc
      301 gggaagtagg acgcgttgag gtagacggtt ttcatgttca ccgcccggtt ggcctcgttc
      361 agctcggtca tctcgtcgag atggcggggc tcctggccga agctgcgcac cacgcgcacg
      421 ccgctgaggc tctcctgcag gtaggcggtg atcgccgcga tccgctcgcg cgtcgcccgg
      481 taggcgccgt gggagacgat ccggaagacg atgctggcga tcagcagcag ggggaaggtg
      541 aggaagacga ccagcgccag ctggacgtcg aggacgagca ggatcacgac caccccgagc
      601 agggtcaggg tgctggagaa gatcgtcacc acgccgtcgg tgaccagctg gttgagcgcc
      661 tcgatgtcgt tggtcatccg cgagatcagg acgccggggc tgcggcgggt gaaaaacccg
      721 atcgacatgc cctggatgtg ctcgaagacg cgctggcgca ggttctggag cacccgggtc
      781 ccgacccaac cgaccatgta ggtctgcagg taggtggccg ccgcgtagag gacggcgacg
      841 gcgaggaagg cggcgacgat cctgtcgagc gcgccgacgt cgccggcgat gatgccggtg
      901 tcgatcgcct gaccggcgag gtagggggga gcgagcccgg cggcggtcgc gccgagcaag
      961 ccgacgaaca tcagcgcgac gcggccgcga tagggacgca gctgcgagac cagccacttg
     1021 accttgcggc cgcgctggtc ctcgccgcga accaggcgcc agagcgccgc gaacagcgcc
     1081 gcgacgttct ggaaggcgtt gcggtcctcg ttcacagtcg cgccatctcc tcgcgctctt
     1141 cgaggtcgcg ttgcaggaag accgagtcgg cgagcccgtg ctcggcgatc tcgcggtaga
     1201 agccacagcc ctccatcagc tcctcgtggg tgccgcggtc gacgatctcg ccgccgtcga
     1261 cgacgacgat ttcgtcggcc agcgagacgg tcgagaggcg gtgggcgatg atgaaagtgg
     1321 tccggcccgc catcgcttct tgcaggccct ccttgatcgc cacctcggtg gtcgcgtcga
     1381 ccgacgaggt ggcgtcgtcg aggatcagga cccggggatc ggcgagcagc gcccgcgcga
     1441 tcgcgacccg ctggcgctgg ccgccggaga gggtcagccc gcgctccccg acccgggtct
     1501 cgtagccgtc gggcagcgcc cggatgaacg cgtcggcctg cgcccgccgc gccgccgctt
     1561 cgatctgctc gcgggtcgcc tcgggctgcg cgtaggcgat gttctcggcg acgctggcgg
     1621 tgaacaggaa gctgtcgtcg gcgacgaagg cgacctcgga acgcagcgac ggcacgtcga
     1681 ccgtgcgcac gtcggccccg tcgaccagca ccgtgccctc gtcggggtcg tacagccgtg
     1741 cgatcagcgc caccaggctg gtcttgcccg agcccgaggg gccgaccagg gcgaccgtgc
     1801 gcccggcctc gacctcgagc gagacgtcgc gcagggccgg cgctgccggc tcgtcggcct
     1861 ccgctgcacc cgcgtagcgc agggtcaccc cgcgcagctc aacccggccg ccgccggccg
     1921 gcagcggcgg cgcgccgggc gcgctctcga tccgcggctc gcggtcgagg atctcgaaca
     1981 gccggttgcc ggaggcgatc gcccgctgcg ccatccccat cgccacgccg agcatccgca
     2041 gcggcccggc cagcatcacc aggtacgtgt agaaggcggt gagttcgccg atgttcatct
     2101 tgtcgtcgat caccaggcgg ccgccgacga gcagcaccag cgcaatgccg atctgcggca
     2161 gcagcccgat cagcggcgag aagaacgcct gcagcttggt cgagtagatg ctctggtcga
     2221 agacgcggga gacggcgcgc tggaagcggt gcagctggtg ctcctcgcgt gcgaacgcct
     2281 tgacgatgcg gatcccggag acgttctcct cggcctccgc ggtcagttcg gcgatccgct
     2341 gctggacctc ctgctgagcc ggacgggaga cgcggttgta gcgcgaggcg gtaaagacga
     2401 tcagcggcgc tggcgccagc gccagcagcg ccagcagcgg gtcgatgaag aacatcaccg
     2461 tgccggccag ggcgatcgtc agcaggttct gggtgatgaa gatcaggccg tagccgagga
     2521 agaatcggat cgcgccgagg tcgacggtcg cccgcgacat cagctggccg gtctgctggc
     2581 cgtcgaagaa accgagctcc agccgctgca ggtgcgcgta gaacttctgc cgcaggtcga
     2641 attccaccgc cagcgagacc ttgccggcga ccaggcgccg cacgacggtg aggccgaggc
     2701 gcaggatcgc ggcgccgagg atcgccaatg ccagcggcag caagtcgggc ttttcttcct
     2761 cgacgatcgc gttgacggcg cgcccgatca gccacgggat cagcaccgtc atccccatcg
     2821 ccgcccaggc aaagaccagc gatccccaga gctgcagccg gtagggacgc aaaaagccca
     2881 tcaaacgcct gtaagtgcgc atcagcactc actatacaaa gtgaagtatt ggtgcttgcg
     2941 ggcgccgccg ggccacccgc gcacctccag gcgcaggcag gcgcacgcgc ggcccggctg
     3001 gacggaacgg actgaagaac ccgggcgccc gggcatcgcg tggttcctta tccgcccata
     3061 tgtccggaaa ggaaccacgc gatccagcgc cccggctccg aacgcagttc ttcccggtat
     3121 gcgggaacaa ctgcgttcga gccctcgcca cggcccgaaa gccagatgga acccccggga
     3181 cggcccgtgt ggaggggtcg ggtaacccct cggaacgtct tgtacttttt tgcttcagcg
     3241 atccgaggcg tctagccgcc gctcgctccc acaaaaaagt taagtggaga gggggtaccc
     3301 gacccctccc cgcatgtcgc cccgaggtac gcccgtcagc gcagctgcgc cgcggccttg
     3361 gcgctgggga tcttgccctt tgggcacggt ttcggtccgt ggccgggctt gggcaggacc
     3421 tggaagttgg tcaccgcgtg gtgggcggtc ggggcgccgc tgttctgcgc cagggtgacg
     3481 accaggcggt aaaagcccgg attgaggttg tggtagtaga tctccggtcg cgtccccggc
     3541 gagtagctgc tggcggtgcc gagctcctcg gcgagcactc cgttgggtcc ggagaactcc
     3601 gggtagtcca tcgaagcgcc gatcagccgg ccgctgccga ccgggctgtg cacggcgcgc
     3661 agcagtttct cgcgctcgac gcagtcgggg acgcggttga ggctgtagcg gacgtggccc
     3721 tggccgagct gcggttcgtt gccgaactgc aggggggcga gggtgaagtt ctcgatgtcg
     3781 accttgacca cgaccgcgtg gctcgactgg cgggcgccgt tgcgcggaga gatgatctcg
     3841 atgctgggcg tgcccggcgc ggcttccgtc ttcgcctggg cctgttttga ctcgccctcg
     3901 ccgaagatca gcgccagcgc gatcgccacc acggcagccg ccagcgccgc gatcgttgcg
     3961 gtcctcagtc gcatcgcagc gaagactacg gatcccactc gcggccgcga gtgccggtaa
     4021 tctcgcccgg gtgacggggg ccaccgaact cgtgtaccgc attggcgcgg ccgcccgcca
     4081 tcccgcgaac tggctgcagc tgttcaagtt cggactggtc ggcggctccg gctacctgat
     4141 caacctcgcc gtcttcgcgt tcgccaccgg cgtcctcgac atccaccacc ttttggccgc
     4201 ggtgctcgcc ttcgtcgtcg ccgtctccaa caacttctgg tggaaccggc actggacctt
     4261 tgccgccggc gacggcgccg ttcacttcca ggccgcccgc ttcttcacgg tcagcatcgc
     4321 cggcctcggg atcaacctcg ccgtgctcga ggcgcttgtc gcctccaaca cggtcggcga
     4381 cctgcccgcc caggcgatcg cggttgcggt cgcgatgccg ttcaacttcg tctgcaaccg
     4441 cctctggacc ttcgcctgag cgtttgcccg ctcagctacc gttgaggcca tgagcggtaa
     4501 caagccaact tacgaccctg cgcgaatcga gccgcagcgc caggaggcct gggagcagcg
     4561 cggcgactac tcggcgccgc cgcggccgga gccgggtcag acgccgatct acgtcaaggc
     4621 ctcgagcccg ttcacctcgg gcaacctgca catgggccac gtccgcgact actcgctcgg
     4681 cgacgcctac gcccgcttcc accgcgcccg cggcgacgcg gtcctgctcg gcttcggctt
     4741 cgacgccttc gggctgccgg ccgagctggc ggcgatcgag cgccaggtgc tgccctccga
     4801 ctgggtgcgc cagtgcggcg accgcatgct cgcccagatg aagcggctcg gctactcctt
     4861 cgactacgac cgcgttttct acagctccga cgagagcttc taccgctggt cgcagtggct
     4921 gttcctgacc ctgctggaga tggacatgat ctacagcgag gacgccaccg ttgactggtg
     4981 cgacacctgc cagacgaccc tggcgacgat ccaggtcgag gagggcggca cctgctggcg
     5041 ctgccacaac gaggtgcggc tgatccggcg cccaacctgg ttcctgcgga tcacgcccta
     5101 cctcgaggag aacgacgaga acaccggcaa gctggcgaac tggaacgagc tggcgctcgc
     5161 cacccagcac tacatcctcg gccgcagcga gggcgtcgag cttgagctag agggcagcgg
     5221 cggcaagctc accgtgttca cgccgcatcg cgagttcgcc ggccacgcca gcttcgtcct
     5281 gctctcgccg cgccatcccg aggtcgacgc ttggatcgcc gacgacgagg tgcgcgagca
     5341 gctcgagcag atgcgctcgg ggggttggga gcgcagcgct cgcgacgcca gcgccgtgcc
     5401 gctgttcgac accggcgcga cgctggccgg accgggtggg ggagagctgc cggtcctcgt
     5461 ctcgcccctg gtcgacgccc gcttcgggcc gaccgcggcg cttggcatcc cgctggtcga
     5521 cgaggccgac cgcgacctcg ccgcccgcgc tgagcgcgta gccaccttcg aggcagcgga
     5581 ggagggggag gcgcccgcgc cggctccggt cgccgccgcc ggtgaggcga agcgctaccg
     5641 cgccaacgac ttctcgatct cccgccagcg ctcctggggc acgccgatcc cgatcatcca
     5701 ctgcgagagc tgcggcgtcg tcccggtccc ggtcgaggac ctgccggtga agctgccgcg
     5761 tgacctggtt ccgagcggcg agggcaaccc gctggccgag cgccccgact tcgtcgacgt
     5821 cgcctgtccc aaatgcggcg agccggccaa gcgcgagacc gacacgctcg actgccactt
     5881 cgatgcgctc ttcctctggg tgccggcggc ggtgccgccg gaggcgcggg ccgagcagat
     5941 gttc
//
LOCUS       DASRIG010000368         8883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1736017, whole genome shotgun sequence.
ACCESSION   DASRIG010000368 DASRIG010000000
VERSION     DASRIG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736017"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFB52_09890"
     CDS             <1..897
                     /locus_tag="VFB52_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06689.1"
                     /translation="DRGNPLRAARAGFEALAAVGAARGVLRKRGADVVMGGGGFVAGP
                     AGLAAALTRTPLVLTEADSHMGLANRLLAGRARRVCLAFPIAGREGERYLVTGRPVPA
                     GVVGGDRATARERLGVPTEARCLMVFGGSQGARSINFAALEAFAERAGRDFHVVHLSG
                     RRDHEELAGRLVAAPYKGDYRLSPYEPDFGAVLAASDLVLGRSGGSVFEVLAAGRPEI
                     LVPYPHATADHQAANAEWMREQGAATVIADAELDADRLASEVAELFGDPERLERMAAA
                     ARRVAKPDAARRIADEVLAAAR"
     gene            894..2231
                     /locus_tag="VFB52_09895"
     CDS             894..2231
                     /locus_tag="VFB52_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HZO06690.1"
                     /translation="MSNGAIGDWHGRRLHFIGIGGAGMSGLALVCARLGATVTGSDRS
                     DSSYMERLRAAGLEPAIGHDAANLPEGAEVVVSTAIGEDNPELALARERGVEPIHRGQ
                     LLAALCAEKRLIAIAGTHGKTTTTAMTVWALRAAGADPAFFVGGEVPGLGPEGEAVNA
                     GWGEGEWVVAEADESDGSFLRLQPEVAVITNVEMDHHTHWGSLAALHTAFRDFADRAS
                     TAVLPADGSLDALAGSAEIVGFDTAVPGPEELPLAVPGQHNLANARAALAAIGAAGID
                     PELEPAHSLAEFPGVHRRLELKGKRDSTRIYDDYAHHPTEVRAALSALRELEPERLIA
                     VFQPHLYSRTKALATQFGAALALADEAVVLDVYPAREQPVGELAGVSGLQVAQAAAER
                     MGGRRVVWLPSAAAAESYLADRLDAESRQAVLVTIGAGDVFRIGEALVAGATR"
     gene            2228..3142
                     /gene="murB"
                     /locus_tag="VFB52_09900"
     CDS             2228..3142
                     /gene="murB"
                     /locus_tag="VFB52_09900"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZO06691.1"
                     /translation="MSGPPPGVRADVPLAPLTKVRTGGTADWFAAPTDAAELVEALRW
                     AQDQGQPVGLVGSGSNLLVADDGFHGLAIKLDGDLAAFERDGERVLAGGAARLPSVAA
                     KTAGWGLSGLEFGVNIPGTVGGSVRMNANAYGGQLARVLEWVEVTTAEGSVRRKPEEL
                     GFVYRNSNLREGEVVSRASFRLRPEDPASIRATLATMRARRREDQPSGIKTFGSTFKN
                     PEDERAGGRTAGQLLDDAGCRGLSEGGARFSEKHANFVENFAEATTADVLALMAAGRR
                     RVHERTGVELEPEVQVLGEAEWPGDWEL"
     gene            3139..3666
                     /locus_tag="VFB52_09905"
     CDS             3139..3666
                     /locus_tag="VFB52_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZO06692.1"
                     /translation="MKRALAVLALVLIAAAAVYYLFVRDKEVAATVQPLALAGTIGSG
                     EDAVVVNGRGEVLTWLTIPEDLTLPELPLDEVPKSGRVKGTVLEQAQILAATPTALRP
                     YVARSRYGESGVDVELTSGIELRFGDASQAKRKWEAAAAVLASEEIEALDYVDLHAPG
                     RPAVDGEGVILPPLG"
     gene            3940..5019
                     /gene="ftsZ"
                     /locus_tag="VFB52_09910"
     CDS             3940..5019
                     /gene="ftsZ"
                     /locus_tag="VFB52_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZO06693.1"
                     /translation="MESTYLAVIKVVGAGGGGTNAVSRMIDAGIRGVEFVAVNTDAQA
                     LQACDADIKLSIGQELTRGLGAGGNPDVGAGAAAESRDEIKEALKGADMVFVTAGEGG
                     GTGTGSAPVIAEIAKEEIGALTVGAVTKPFEFEGKRRMQQALEGIEKLRNNVDTLIIV
                     PNEKLLQAVERRTSVLEAFRQADDILRQGVQGITDLITIPGLINLDFADVRTIMRDAG
                     SALMGVGTASGEKRALDAAKLAITSPLIEESIKGATGMLLNITGPEDLGLFEVNEAAQ
                     LVQAEADDNANIIFGSVVDQSMGDEIRVTVIATGFEGFELLARSPQRVRRRYESRPRV
                     DAEDREIRKLRVSDDDIDVPGFLKE"
     gene            5034..6113
                     /gene="gcvT"
                     /locus_tag="VFB52_09915"
     CDS             5034..6113
                     /gene="gcvT"
                     /locus_tag="VFB52_09915"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZO06694.1"
                     /translation="MSDSALRRTPLYEQHVELGAKVVPFAGWEMPVTYEGIREEHSAV
                     RTHVGMFDVSHMGEVEVEGPGALAFLQRVLSNDVSKVEIGGAQYSCLCNEDGGVIDDL
                     FTYRLGGDRYLVVTNAANHGTDLAWLGRWSREFDVAVRDVADRYAMLAVQGPHARAVL
                     GPALGIELPPRMHVSHAQIGRRPALVAGTGYTGEDGVEILIDPEVAVPIWVELLEAGV
                     VPCGLGARDTLRLEVCFPLHGNDLTPERNPVEAGLGWCCKEETGFIGSAAVAAARADG
                     TAEKLAPFLIEGQGIPREGNPVLVGGEIVGTVSSGTFSPSLEVGAGMAYVRSDLAEPG
                     TELEIDVRGKHRAARVGSKPLYKRS"
     gene            6115..6504
                     /gene="gcvH"
                     /locus_tag="VFB52_09920"
     CDS             6115..6504
                     /gene="gcvH"
                     /locus_tag="VFB52_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZO06695.1"
                     /translation="MADESYPEELRYHDEHDWVRIEGDEATFGITWYAQDSLGEVVFW
                     EGPEAGATVQKDSSYAEVESVKAVSDVIAPMSGEVVEINDVLADAPEKINEDPYGEGW
                     LVKVKLSSPEEADTLLGAAEYRKLLDS"
     gene            6544..7914
                     /gene="gcvPA"
                     /locus_tag="VFB52_09925"
     CDS             6544..7914
                     /gene="gcvPA"
                     /locus_tag="VFB52_09925"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZO06696.1"
                     /translation="MSRYTSATPEDRAEMLAAIGADSVADLFEQIPEPLRLGRPLAMP
                     DGLSEAEVYERLAGLAARNADAEAEVCFVGAGMYDHYVPAIVDAITSRSEFLTPYTPY
                     QPEISQGGLQVMFEFQTAMSELTGLPVSNAGLYEGPSSVASAGYLAMGATKRRKFVVS
                     RGVHPHSRETLETYSEGYGAEVVEVGLEGGVTDAAALAEAVDGETAAVILQSPNFLGA
                     VEDVEALGAAAKEHGALLVVACDPMTLGILKPPGAQGADIALGEGQPLGNRLDFGGPS
                     FGFFCATEEQIRRMPGRIAGETEDVDGRRGFVLALQTREQHIRREKATNNICTAQALN
                     ALGAMIHLAWLGRHGFRELGELLVRRTAYARERLAAVDGVSLLHEAPVVREFALALDA
                     PVGKVLDRVAERGIAAGYPLGREYPEHENGLLVAITERRTRAQIDELAEALGAAVAAE
                     REGVAA"
     gene            7914..>8883
                     /gene="gcvPB"
                     /locus_tag="VFB52_09930"
     CDS             7914..>8883
                     /gene="gcvPB"
                     /locus_tag="VFB52_09930"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZO06697.1"
                     /translation="MALTETPMQRERATTIYEKSKPGRRAAVLPAAEVPETPLAELIP
                     AHLLREQEPNLPEIAEPEIVRHYNRLSRRNFDLDSGFYPLGSCTMKHNPRLNERVAAL
                     PGHARLHPAQDPRRAQGALELMWLLQESLSEICGLPHVSLQPSAGSHGELAGLLLTRA
                     YHADRGEERHKVLAPDTSHGTNPASVTMAGYETVKVSTNGQGGVEMDDLRAKLAEHAG
                     DVACLMLTNPNTLGLFDENIAEIASLIHAEGGTLYYDGANLNAVMGQSRPGDMGFDIV
                     HVNLHKSFSQPHGGGGPGAGPIAVSERIAPFLPRPQVVRREADNGHG"
BASE COUNT         1331 a         3230 c         3154 g         1168 t
ORIGIN      
        1 gaccgcggca acccgctacg cgcggcgcgg gcggggttcg aggcgctggc cgcagtcggc
       61 gcggcgcgcg gagtcctgcg caagcgcggc gccgacgtgg tgatgggcgg cggcggcttc
      121 gtcgccgggc cggccggcct ggccgcggcg ctgacccgta cgccgctggt cctgaccgag
      181 gccgacagcc acatgggcct cgccaaccgg ctgctggccg gacgggcgcg gcgggtctgc
      241 ctggccttcc cgatcgccgg acgggagggg gagcgctacc tggtgaccgg gcgtccggtc
      301 cccgccggcg tcgtcggcgg cgaccgcgcg acggcgcgcg agcggctcgg ggtcccgacc
      361 gaggcgcgct gcctgatggt cttcggcggc agccagggcg cccgctcgat caacttcgcc
      421 gcgctggagg cgttcgcaga gcgggccggc cgcgacttcc acgtcgtcca cctcagcggc
      481 cgccgcgacc acgaggagtt ggccgggagg ctcgtcgccg ctccatataa aggggactac
      541 cggctgagcc cctacgagcc cgacttcggc gccgtgctcg ccgcctccga tctggtcctc
      601 ggtcgctccg gcggctcggt cttcgaggtg ctggcggcag gccgcccgga gatcctcgtg
      661 ccctacccgc acgcgaccgc cgaccaccag gcggccaacg ccgagtggat gcgcgagcag
      721 ggagcggcga cggtgatcgc cgacgccgag ctcgacgccg accggctcgc gtccgaggtg
      781 gcggagctgt tcggtgaccc ggagcggctg gagcggatgg cggcggcggc acggcgggtg
      841 gcgaagcccg acgcggcgcg tcggatcgcc gacgaggtcc tggcggcagc acgatgagca
      901 acggggcgat cggcgactgg cacggccggc ggctgcactt catcgggatc ggcggtgccg
      961 ggatgagcgg cctggccttg gtctgcgccc ggctcggcgc cacggtcacc ggcagcgacc
     1021 gctcggactc ctcttatatg gagcgcctgc gcgctgcggg gctcgaaccg gcgatcggcc
     1081 acgacgccgc caacctgccc gagggcgccg aggtggtcgt ctcgaccgcg atcggcgagg
     1141 acaacccgga gctggccctg gcccgcgagc gtggggtgga gccgatccac cgcggccagc
     1201 tgctggccgc gctctgcgcc gagaagcggt tgatcgcgat cgccggcacc cacggcaaga
     1261 ccacgaccac ggcgatgacg gtctgggcgc tgcgcgccgc cggcgccgac ccggccttct
     1321 tcgtcggcgg cgaggtgccc gggctcggcc ccgagggcga ggcggtcaac gccggctggg
     1381 gtgagggcga gtgggtcgtc gccgaggccg acgagagcga cggcagcttc ctgcgcctgc
     1441 agcccgaggt cgcggtgatc accaacgtcg agatggacca ccacacgcac tggggctcgc
     1501 tcgccgccct gcacaccgcc ttccgcgact tcgccgaccg cgcctcgacc gcggtcctgc
     1561 ccgccgacgg ctcgctcgac gcgctcgcgg gctccgccga gatcgtcggc ttcgacaccg
     1621 cggtgccggg cccggaggag ctgccgctcg ccgtccccgg tcagcacaac ctcgccaacg
     1681 cccgcgccgc gctcgccgcg atcggcgccg ccgggatcga ccccgagctg gagccggcgc
     1741 actcgctggc cgagttcccc ggagtgcacc gtcgtctcga gctcaagggc aagcgcgaca
     1801 gcacccgcat atatgacgac tacgcgcacc acccgaccga ggtgcgggcg gcgctctcgg
     1861 ccctgcgcga actcgaaccg gagcgcctga tcgccgtctt ccagccgcat ctctactcgc
     1921 gcaccaaggc gctggcgacc cagttcggcg ccgccctggc gctcgccgac gaggcggtcg
     1981 tgctcgacgt ctaccctgcc cgcgagcagc cggtcggcga gctcgccggg gtcagcgggc
     2041 tgcaggtcgc gcaggccgcg gccgagcgga tggggggcag gcgggtcgtc tggctgccta
     2101 gcgccgccgc ggccgagagc tacctcgccg accgtctcga cgccgagtct cgccaggcgg
     2161 tcctggtcac gatcggcgcc ggcgacgtct tccggatcgg cgaggccctg gtcgcggggg
     2221 ccacgcggtg agcgggccgc cacccggggt gcgcgccgac gtgccgctcg ccccgctgac
     2281 caaagtccgc accggcggaa ccgccgactg gttcgccgcc ccgaccgacg cggcggagct
     2341 ggtcgaagcg ttgcgctggg cgcaggatca gggtcagccc gtaggactgg tcggctccgg
     2401 ctcgaacctg ctcgtagccg acgatggatt ccacggactc gcgatcaagc tggacggtga
     2461 cctcgccgcg ttcgagcgcg acggtgagcg cgtgctcgcc ggcggcgctg cgcgcctgcc
     2521 ctcggtcgcg gccaaaaccg ccggctgggg actctccggg ctcgagttcg gcgtcaacat
     2581 ccccggcacg gtcggtggct ccgtgcgcat gaacgcgaac gcctacggcg gccagctcgc
     2641 ccgcgtgctc gagtgggtcg aggtgaccac cgccgagggc agcgtgcgcc gcaagcccga
     2701 ggagctcggc ttcgtctacc gcaactcgaa cttgcgcgag ggcgaagtcg tcagccgcgc
     2761 cagctttcgc ctgcgccccg aggacccggc ttcgatccgg gcgacgctgg cgacgatgcg
     2821 cgcccgccgg cgcgaggacc agccctccgg gatcaagacc ttcggctcga ccttcaagaa
     2881 ccccgaggac gagcgcgccg gcggccgcac cgccgggcag ctgctcgacg acgccggctg
     2941 ccgcggcctc agcgagggcg gcgcccgctt ctccgagaag cacgccaact tcgtcgagaa
     3001 cttcgccgag gcgacgaccg ccgacgtgct ggcgctgatg gccgccggcc gccgccgcgt
     3061 gcacgagcgg accggggtcg agctggagcc ggaggtccag gtgctgggcg aggccgagtg
     3121 gcccggcgac tgggagctgt gaagcgcgcg ctcgcagtcc tcgcgctcgt cctgatcgcg
     3181 gccgcggccg tctactacct cttcgttcgc gacaaggaag tcgccgcgac cgtgcagccg
     3241 ctggcgctgg ccgggacgat cggcagcggc gaggacgcgg tcgtcgtcaa cggtcgcggc
     3301 gaagtgctca cctggctgac catccccgag gacctgaccc tccccgaact gccgctcgac
     3361 gaggtgccga aaagcggccg ggtgaagggc acggtgctgg agcaggcgca gatcctggcg
     3421 gcgacgccga cggcgctccg cccctacgtc gcccgcagcc gctacggcga aagcggggtg
     3481 gacgtcgagc tcacctcggg gatcgaactg cgcttcggcg acgcctcgca agcgaagcga
     3541 aagtgggaag cggccgccgc cgtcctcgca tccgaggaaa tcgaagcgct cgattatgta
     3601 gacctgcacg ctccggggcg ccccgcggtc gacggagaag gggtcatcct gccgccgctt
     3661 ggctgagccc aaagcccctc catgcctaag gttcgacctt ggagtgaggg tcggaaaacc
     3721 ctcaactcat ggtcgagagt tatcgcttgc aggatgccat tcggccgttt gacagagcgg
     3781 caggaatgcc ctacgttgag ccgaatttcc gatgtaacag cggagcctga accttcaagc
     3841 atcgctgcag agtcggacag caccgggctc aactcaaaac caagacttgg ctgaacgccg
     3901 acccatcccc aggtcggtac agctggagaa aatcgggaga tggaatccac ttacctcgcg
     3961 gtcatcaagg tcgtcggagc aggcggcggc ggcaccaatg cggtcagccg gatgatcgac
     4021 gccggcattc gcggcgtcga gttcgtcgcg gtcaacaccg acgcgcaggc gctgcaggcg
     4081 tgtgacgccg acatcaagct ctcgatcggg caggagctga cccgcggcct cggcgccggc
     4141 ggcaaccccg acgtcggagc gggcgccgcg gcggagagcc gcgacgagat caaggaggcg
     4201 ctgaagggcg ccgacatggt cttcgtcacc gccggcgagg gtggcggcac cggcaccggc
     4261 tcggccccgg tgatcgccga gatcgccaag gaagagatcg gcgccctgac cgtcggcgcg
     4321 gtgaccaagc cgttcgagtt cgagggcaaa cgacggatgc agcaggcgct cgagggcatc
     4381 gagaaactgc gcaacaacgt cgacaccctg atcatcgtcc cgaacgagaa gctgctgcag
     4441 gcggtcgagc ggcgcacctc ggtgctggag gcgttccgcc aggccgacga catcctgcgc
     4501 cagggcgtcc agggcatcac cgacctgatc acgatccccg gcctgatcaa cctcgacttc
     4561 gccgacgtgc gcacgatcat gcgcgacgcc ggctcggcgc tgatgggcgt cggcaccgcc
     4621 tcgggcgaga aacgcgcgct cgacgccgcc aaactggcga tcacctcgcc gctgatcgag
     4681 gagtcgatca aaggcgcgac cgggatgctg ctgaacatca ccggccccga ggatctcggt
     4741 ctgttcgagg tcaacgaggc cgcgcagctg gtccaggccg aggccgacga caacgccaac
     4801 atcatcttcg gctcggtcgt cgaccagtcg atgggcgacg aaatccgcgt caccgtgatc
     4861 gccaccggct tcgagggctt cgagctcctc gcccgctcac cgcagcgggt gcgccgtcgc
     4921 tacgagtcgc gcccccgcgt cgacgccgag gaccgggaga tcaggaaact tcgcgtgtcc
     4981 gacgacgaca tcgacgtccc cggcttcctc aaggaataaa taggctgggt cgcgtgagcg
     5041 actcggccct ccggcgcacc cccctctacg agcagcacgt cgagcttggg gcgaaggtcg
     5101 tgcccttcgc cggctgggag atgccggtca cctacgaggg catccgcgag gagcactcgg
     5161 cggttcgcac ccatgtcggc atgttcgacg tctcgcacat gggcgaggtc gaagtcgagg
     5221 gtccgggcgc gctcgccttc ctgcagcgcg tgctctccaa cgacgtctcc aaggtcgaga
     5281 tcggcggcgc ccagtactcc tgcctctgca acgaggacgg cggcgtgatc gatgacctct
     5341 tcacctaccg gctcggcggc gaccgctacc tggtcgtcac caacgcggcc aaccacggga
     5401 ccgacctcgc ctggctcggc cgctggagcc gcgagttcga cgtcgccgtg cgcgacgtcg
     5461 ccgaccgcta cgcgatgctc gccgtgcagg gcccgcacgc ccgcgccgtg ctcggcccgg
     5521 cgctggggat cgagctgccg ccgcggatgc acgtatccca cgcgcagatc ggccgccgcc
     5581 cggcgctggt cgccggcacc ggctacaccg gcgaggacgg ggtcgagatc ctgatcgacc
     5641 ccgaggtcgc ggtgccgatc tgggtcgagc tgctcgaggc cggcgtcgtc ccctgcgggc
     5701 tcggcgctcg cgacacgctc cgcctggagg tctgcttccc gctgcacggc aacgacctga
     5761 cgcccgagcg caacccggtc gaggccgggc tcggctggtg ctgcaaggag gagaccgggt
     5821 tcatcggctc cgcggccgtc gccgccgccc gcgccgatgg caccgccgag aagctcgccc
     5881 cgttcctgat cgagggccag gggatcccgc gcgaaggcaa cccggtcctg gtcggcggcg
     5941 agatcgtcgg cacggtcagc agcggcacct tctcgccctc gctcgaagtc ggcgccggga
     6001 tggcctacgt gcgcagcgac ctggccgagc cggggaccga gctggagatc gacgtccgtg
     6061 gcaagcatcg ggcggcccgg gtagggtcga aacccctcta caaaaggagc tgaattggcc
     6121 gacgagagtt atcccgagga gctgcgctac cacgacgaac acgactgggt tcggatcgag
     6181 ggcgacgagg ccactttcgg gatcacctgg tacgcccagg actcgctcgg cgaggtcgtc
     6241 ttctgggagg ggcccgaagc cggcgccacc gtgcagaaag actcctccta cgcggaggtc
     6301 gagtcggtca aggcggtctc cgacgtgatc gcgccgatgt ccggcgaggt ggtcgagatc
     6361 aacgacgtgc tcgccgatgc gccggagaaa atcaacgagg acccctacgg cgagggctgg
     6421 ctggtcaagg tcaagctgag ctccccggag gaggccgaca cgctgctcgg cgccgccgaa
     6481 taccgcaagt tgctggatag ctagggcgaa gccgcgcccc tcgcttccgt agccttagac
     6541 gcagtgagcc gctacacctc cgccacgccc gaggaccgcg ccgagatgct ggccgcgatc
     6601 ggggccgact cggtcgccga cctgttcgag cagatccccg agccgctgcg cctcgggcgc
     6661 ccgctggcca tgcccgacgg cctcagcgag gccgaggtct acgagcgcct cgcgggcctg
     6721 gcggcgcgca acgccgacgc cgaggccgag gtctgcttcg tcggggcggg gatgtacgac
     6781 cactacgtgc cggcgatcgt cgacgcgatc acctcgcgct cggagttcct cacgccctac
     6841 acgccctacc agccggagat ctcacagggc ggtctgcagg tgatgttcga gttccagacc
     6901 gcgatgtcgg agctgaccgg cctgccggtc tccaacgccg gcctctacga ggggccctcg
     6961 tcggtcgcct ccgccggcta cctggcgatg ggcgcgacca aacgtcgcaa gttcgtcgtc
     7021 tcgcgtggtg tgcacccgca cagccgcgag accctggaga cctactccga gggctacggc
     7081 gccgaggtgg tcgaggtcgg gctcgagggt ggagttaccg acgccgctgc gctcgctgag
     7141 gcggtcgacg gcgagaccgc cgccgtgatc ctgcagagcc ccaacttcct cggcgcggtc
     7201 gaggacgtcg aggcgctcgg cgcggccgcc aaggagcacg gcgcgctgct ggtcgtcgcc
     7261 tgcgacccga tgaccctggg gatcctcaag ccgcccggcg cccagggcgc cgacatcgcc
     7321 ctcggcgagg gccagccgct cggcaaccgc ctcgacttcg gcggcccctc gtttggcttc
     7381 ttctgcgcca ccgaggagca gatccgccgc atgccggggc ggatcgccgg cgaaaccgag
     7441 gacgtcgacg gccgccgtgg cttcgtcctc gcgctgcaga cccgcgagca gcacatccgc
     7501 cgcgagaaag cgaccaacaa catctgcacc gcgcaggcgc tgaacgcgct cggcgcgatg
     7561 atccacctcg cctggctcgg ccgccacggc ttccgcgagc tcggcgagtt gctggtccgc
     7621 cgcaccgcct acgcgcgcga gcgcctggcc gccgttgacg gagtctcgct gctgcacgag
     7681 gcgccggtcg tgcgcgagtt cgcgctcgcg ctcgacgcgc cggtcggcaa ggtcctcgac
     7741 cgcgtcgccg agcgcgggat cgcggccggt tacccgctgg gccgcgagta ccccgagcac
     7801 gagaacggtc tcctcgtcgc gatcaccgag cggcgcaccc gcgcccagat cgacgagctg
     7861 gccgaggcgc tcggcgccgc cgtcgccgcc gagcgcgagg gggtggctgc ctgatggcgc
     7921 tgaccgagac cccgatgcag cgcgaacggg cgacgacgat ctacgagaag tcgaagccgg
     7981 gccggcgcgc cgccgtgctg cccgccgccg aagtcccgga gacgccgctg gcggagctga
     8041 tcccggccca cctgctgcgc gagcaggagc cgaacctgcc ggagatcgcc gagccggaga
     8101 tcgtccgcca ctacaacagg ctctcgcgcc gcaacttcga cctcgacagc ggcttctacc
     8161 cgctcggctc gtgcacgatg aagcacaacc cgcggctcaa cgagcgggtc gcggcgctgc
     8221 cgggccacgc ccgcctgcac ccggcccagg acccgcggcg ggcccagggc gctctggagc
     8281 tgatgtggct gctgcaggag agcctcagcg agatctgcgg cctgccccac gtcagcctgc
     8341 agccctcggc cggctcgcac ggcgagctcg ccggcctgct cctgacccgc gcctaccacg
     8401 cagatcgcgg cgaagagcgc cacaaggtgc tcgcccccga cacctcgcac gggaccaacc
     8461 cggcctcggt gacgatggcc gggtacgaga cggtcaaagt gtcgaccaac gggcaaggtg
     8521 gggtggagat ggacgacctg cgggcgaagc tggccgagca cgctggcgac gtcgcctgtc
     8581 tgatgctgac caacccgaac acgctcgggc tgttcgacga gaacatcgcc gagatcgcct
     8641 cgctgatcca cgccgagggc ggcactctct actacgacgg cgccaacctg aacgcggtga
     8701 tgggccagtc gcggcccggc gacatgggct tcgacatcgt ccacgtcaac ctgcacaagt
     8761 cgttcagcca gccgcacggc ggcggcggcc ccggggccgg cccgatcgcc gtctccgagc
     8821 ggatcgcgcc cttcctgccg cgcccccagg tggtccgccg cgaggccgac aacggccacg
     8881 gcc
//
LOCUS       DASRIG010000369         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3849357, whole genome shotgun sequence.
ACCESSION   DASRIG010000369 DASRIG010000000
VERSION     DASRIG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849357"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFB52_09935"
     CDS             complement(<1..420)
                     /locus_tag="VFB52_09935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZO06698.1"
                     /translation="MAKLQVGDPAPDFELPGTGGKTYRLADYRGRKLIIAFYPGDFTA
                     VCTKQFCSYRDEGERLDQLGADVLGISPQSVESHERFTEQHGLRVPLLSDPNKAVARA
                     YGVVGPGGFLRRSVFLVDGEGIVRYRHVALFGLRYQDV"
     gene            complement(431..1129)
                     /locus_tag="VFB52_09940"
     CDS             complement(431..1129)
                     /locus_tag="VFB52_09940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZO06699.1"
                     /translation="MEAQQPDRRWIILGVFALLAIVVVGVILISRGGGDDDGGDTTSA
                     SSGSKGGCEKVEAPEPKTVSFSKPDEVLEKGEKVNAVVATSCGEFTIALDTERAPKTS
                     NSFAFLAEEGFFDETTFHRIVPEFVIQGGDPEGTGGGGPGYSVVEAPPSNLEYTKGIV
                     AMAKTETEAPGTSGSQFYVVTGANVGLPPEYALLGEVTDGYPTVEKIEKQAEPGSESP
                     KQTILIESITIEKE"
     gene            1170..1676
                     /locus_tag="VFB52_09945"
     CDS             1170..1676
                     /locus_tag="VFB52_09945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO06700.1"
                     /translation="MSSPPLTPLATATLAVGRPESALVVECGDGDAALFLAREFPQAR
                     VRGVDTDEGAIRRATARIGLDPEGRIAFKVGGPRALPFPDDHFDLVAVLDGRLAAAET
                     ARVLRPGGFLVIAYSERLHAPSKLGSRLGEWRFSRQGFVAVESEEAGDGSFSVARLGK
                     ADRSRGAE"
     gene            1686..2576
                     /locus_tag="VFB52_09950"
     CDS             1686..2576
                     /locus_tag="VFB52_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZO06701.1"
                     /translation="MAIDGMPLAIFVNPHSAGGKTIKLLPRVEQVLDERRVEFRVQRT
                     KGLEHGVEQALLAVEAGELPVVMSGDGLVGAIGGAMAGSETPLGILPGGRGNDLARVL
                     GIPSEPEEAIDVVLGGQTRRIDVGEANGKRFLGIVSVGFDSEANRRANENKFLRGSLV
                     YAWAAIVTLVKWKPARYTIRVDDERTRITGYSISVANSSCFGGGMYVAPDALLDDGEF
                     DVVSVGDVSKLRYVSNLPKVFKGTHVEEDEVVRVFRAKHLELSASRPFPVYADGEHLT
                     ELPAKLRVLPRALSVLVPGA"
     gene            2573..>3898
                     /locus_tag="VFB52_09955"
     CDS             2573..>3898
                     /locus_tag="VFB52_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZO06702.1"
                     /translation="MSAGFSTKVALARAVGKASRASGRGGGTTLPGRVLLRLEPEAIA
                     ELGHRLGAGSTVISATNGKTTTAGMVAAILAADGRQPVHNRAGSNMTWGVATALLEQR
                     GEEGLFEVDEAWLPRVSEQLGPKLIVLGNLFRDQLDRYGEMEALADEWAKTVAAREGS
                     SGFALNADDPLIADLGRDPEGRPREGVTYFGIDDPAQALPELQHAFDAKHCRRCGHPY
                     EYERAFVGHLGHYSCPNCGAKRPRPDVSATEIELRGMDGSRSRVRTPAGEIELELPLP
                     GLYNVYNALAAIAAGLRLGVSPERIGAALGEMRAAFGRVETIAVGDAQVSILLIKNPA
                     GANEVLRTLRLEADGEGEGIDLWIALNDRIADGRDVSWVWDADFELLAGAVRRVVCSG
                     TRAPEMALRLKYAGWPTDRIEVIDGIEASLDAAVSDRRVFALPTYTALLE"
BASE COUNT          566 a         1348 c         1388 g          596 t
ORIGIN      
        1 cacgtcctgg tagcggaggc cgaacagagc cacgtggcgg tagcgcacga tcccctcgcc
       61 gtccacgagg aacaccgagc gcctcaggaa gccccccggg ccgacgacgc cgtaggcgcg
      121 agcgacggcc ttgttcgggt cggagagcag cggcaccctg agcccgtgct gctccgtgaa
      181 gcgctcgtgc gactcgaccg actgcggcga gatcccgagc acgtcggcgc cgagctggtc
      241 gagccgctcg ccctcgtccc ggtaggagca gaactgtttg gtgcagacgg cggtgaaatc
      301 gcccgggtag aaggcgatga tcagcttgcg accgcggtag tcggcgagcc ggtaggtctt
      361 gccaccggtg ccgggaagct cgaaatcggg agcgggatcg cccacctgga gcttggccat
      421 ccgcgtccgc ctattccttt tcgatcgtga tgctttcgat caggatggtc tgtttcggag
      481 attcggagcc gggttcggcc tgtttctcga tcttttcgac cgtcgggtat ccgtcggtga
      541 cttcgccgag cagcgcgtat tcgggcggca ggccgacgtt ggcgccggtg acgacgtaga
      601 actggctgcc ggaggttccg ggcgcctcgg tttcggtctt cgccatcgcg acgatgcctt
      661 tggtgtattc gaggttgctc ggcggtgcct cgaccacgct gtagccggga ccgccgccgc
      721 cggtgccttc gggatcgccg ccctggatca cgaattccgg gacgatccgg tggaaggtgg
      781 tttcatcgaa gaagccttcc tcggcgagga aggcgaagga gttactggtt ttcggcgccc
      841 gttcggtgtc gagcgcgatc gtgaactcgc cgcagctggt cgccaccacg gcgttcactt
      901 tttcgccttt ctcgagcacc tcgtcgggtt tcgagaagga gacggtcttc ggttcgggcg
      961 cttcgacttt ctcgcagccg cctttggagc cgctggaagc gctcgtggtg tcgccgccgt
     1021 catcgtcccc gccgccgcgc gagatcagga tcaccccgac cacgacgatc gcgagcaggg
     1081 caaaaacgcc gaggatgatc caacgtctat ctggctgctg cgcctccacg gccggagaga
     1141 ttagtgcaaa ccgctttacg atcggggcga tgtcgtcgcc cccgctgacg ccacttgcca
     1201 ccgccacgct cgcggtcggc cggcccgaga gcgctctagt ggtcgaatgc ggcgatggcg
     1261 acgccgccct cttcctcgcc cgtgagttcc cgcaggcgcg cgtgcgcggg gtcgacaccg
     1321 acgagggcgc gatccgccgc gcgaccgccc ggatcgggct cgatccggag gggcggatcg
     1381 ccttcaaggt tggcggccct cgcgccctcc ccttccccga cgaccacttc gaccttgtcg
     1441 cggtgctcga cggacgactc gcggctgccg agacggcccg cgtcctgcgt ccgggcggct
     1501 ttctggtgat cgcctactcc gagcgcctgc acgcgccctc gaagctcggc agccggctcg
     1561 gcgagtggcg cttttcccgc cagggcttcg ttgcggtcga gtccgaggag gccggcgacg
     1621 gcagcttctc tgtcgcccgc ttggggaagg ctgaccggag ccgcggcgcg gaataggctc
     1681 agggcatggc catcgacggc atgccgctgg cgatcttcgt caaccctcat tcggccggcg
     1741 gcaagaccat caagctgctg ccgcgcgtgg agcaggtgct cgacgagcgc cgggtcgagt
     1801 tccgcgtcca gcgcaccaag gggctcgagc acggggtcga gcaggcgctg ctcgcggtcg
     1861 aagcgggcga gctgccggtg gtgatgagcg gcgacgggct ggtcggcgcg atcggcggcg
     1921 cgatggcggg ctcggagacg ccactaggca tcctccccgg cggccgcggc aacgacctcg
     1981 cacgcgtgct cggcatcccc agcgagccgg aggaggcgat cgatgtcgtg ctcggcggac
     2041 agacgcgccg gatcgacgtc ggcgaggcca acggcaagcg cttcctcggg atcgtcagcg
     2101 tcggcttcga ctcggaagcc aaccggcgtg ccaacgagaa caagttcctg cgcggcagcc
     2161 tcgtctacgc ctgggcggcg atcgtcaccc tggtcaaatg gaagccggcc cggtacacga
     2221 tccgggtcga cgacgagcgc acccggatca ccggctactc gatctcggtc gccaacagca
     2281 gctgcttcgg cggtggcatg tacgtggcgc cggacgcgct gctcgacgac ggcgaattcg
     2341 acgtggtctc ggtcggcgac gtcagcaagc tgcgctacgt gagtaacctg ccgaaggtgt
     2401 tcaagggcac ccacgtcgag gaggacgagg tcgtccgcgt cttccgcgcg aaacacctcg
     2461 aactgagcgc cagccgcccc ttccccgtct acgccgacgg cgagcacctg accgagctgc
     2521 cggcgaagct gcgggtgctg ccgcgggcgc tgagcgtgct ggtcccgggc gcttgagcgc
     2581 cggcttctcc accaaggtcg cgcttgcgcg ggcggtcggc aaggcgagcc gcgcgagcgg
     2641 tcgcggcggc ggcaccacgc tgccgggacg ggtgttgctg cgcctggagc cggaggcgat
     2701 cgccgagctc ggccaccgcc tcggtgccgg ctcgaccgtg atctcggcga ccaacggcaa
     2761 gaccacgacg gcgggcatgg tcgccgcgat cctcgccgcc gacggccgcc agccggtcca
     2821 caaccgggcc ggctcgaaca tgacctgggg agtcgccacg gcgctgctcg agcagcgcgg
     2881 cgaggagggc ctgttcgagg tcgacgaggc ctggctgccg cgggtcagcg agcagctcgg
     2941 cccgaagctg atcgtgctcg gcaacctctt ccgcgaccag ctcgaccgct acggcgagat
     3001 ggaggcgctg gccgacgagt gggcgaagac ggtggccgcg cgcgagggca gctccggctt
     3061 cgcgctgaac gccgacgacc cgctgatcgc cgacctcggc cgcgaccccg aggggcggcc
     3121 gcgcgagggc gtcacctact tcgggatcga cgaccccgca caggcgctgc ccgagctgca
     3181 gcacgccttc gacgccaagc actgccgccg ctgcgggcac ccctacgagt acgagcgcgc
     3241 cttcgtcgga cacctcggcc actactcgtg cccgaactgc ggggcgaagc gaccccgtcc
     3301 ggacgtgagc gcaaccgaga tcgagctgcg cgggatggac gggtcgcgca gccgggtgcg
     3361 aacgccggcg ggcgagatcg agctggagct gccactgccc ggtctctaca acgtctacaa
     3421 cgcgctcgcg gcgatcgccg ccggcctgcg gctcggcgtc tccccggagc ggatcggcgc
     3481 ggcgctcggc gagatgcggg ccgccttcgg ccgggtcgag acgatcgcgg tcggcgacgc
     3541 gcaggtctcg atcctgctga tcaagaaccc ggccggcgcc aacgaggtcc tgcgcacgct
     3601 gcgcctggag gcggacggcg agggcgaggg catcgacctc tggatcgcgc tcaacgaccg
     3661 gatcgccgac ggccgcgacg tctcctgggt ctgggacgcc gatttcgagc tgctggccgg
     3721 cgccgtgcgc cgggtcgtct gcagcggcac ccgcgctccc gagatggcgc tgcgcctcaa
     3781 gtacgccggc tggcccaccg accggatcga ggtaatcgac ggcatcgagg cgtccctcga
     3841 cgccgcggtc agcgaccgcc gggtgtttgc gctgccgacc tacacggccc tgctcgaa
//
LOCUS       DASRIG010000370         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2254518, whole genome shotgun sequence.
ACCESSION   DASRIG010000370 DASRIG010000000
VERSION     DASRIG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254518"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VFB52_09960"
     CDS             complement(<1..1568)
                     /locus_tag="VFB52_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HZO06703.1"
                     /translation="MSTQPEQFDVVVLGGGLAGLTLAIQLKRRRAATSVAVLEKRAWP
                     VPEAAHKVGESTLQIGAHYFRETCGMEEHLEREQLRKMGLRFFLATPPGDDVGQRIEM
                     GLAGWADDAPTYQLDRGRFENALAVRAEELGAEVRGGVSVTGVERDGDGTHVVATRDG
                     ELRATWVVDASGRGAILKRKHGLAVEVEHHCNAAWFRIGRRIEIDKWSEDPRWRERVP
                     SGTRWQSTVHFMGPGYWFWVIPLASDSTSFGLVADPDLVPFERLRRFEPLMEWLAEQE
                     PLAAEMLEPHRGELQDFRLLKHYSHGCEQVFSPERWCITGEAGVFLDPLYSPGSDMIA
                     ISNTMITRFVTGSLEGKDADLAIQFFNDLYLLISSRLISTWSGQYPGFARPEITAAKV
                     TWDFMTYFGSLALLAVTGLVAEAKFLVTVLREVDRIAELNLNMQRHFRNWSAQADQGG
                     ESPGFPCISDEAYRRLEADVATIRDPEEVRETIGRNAARIEAAAIEILARSAAALGHE
                     VDPAELDPYTFRLPG"
     gene            complement(1578..>2682)
                     /locus_tag="VFB52_09965"
     CDS             complement(1578..>2682)
                     /locus_tag="VFB52_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO06704.1"
                     /translation="GPRIDRLSIKPAVRAAERGRLWRALGNGVLRHRWAVLLGSVAIL
                     LLCALPVLGTKLVFAGTDGLLPADNQLRQTSDLVNREFDGGLLEPVEVMVRPAYAARA
                     RAAVARDPGIARVLPPQRGADGWVRLDALPAVSGGSVAGDATVRRVRERLAGVAPPGA
                     AYVGGQPALGVDLIDRVQERFGLMILLACALAFVLLLIALRSLVVPLKAVLTNLLSVA
                     ATLGLVTLIFQDLGGADGLAWFVPPFLFAIVFGLSMDYEVFLLSRVREEYDRGVGNDA
                     AVTNALRQSGRAITLAAVVIMLVFLSSAVSSLESFRQLGVGMAIAVLLDATLVRCGLV
                     PAALAVLGDRNWWLPNPLARLFPDRPVSQSATE"
BASE COUNT          402 a          939 c          987 g          354 t
ORIGIN      
        1 ccgggcaggc ggaaggtgta ggggtcgagc tcggcgggat cgacctcgtg gccgagcgcg
       61 gcggcgctgc gggcgaggat ctcgatcgcg gccgcctcga tccgagcggc gttgcggccg
      121 atcgtctcgc gcacctcctc gggatcgcgg atcgtcgcca cgtctgcctc cagacggcgg
      181 taggcctcgt cgctgatgca cgggaagccg gggctctccc cgccctggtc ggcctgcgca
      241 ctccagttgc ggaagtgccg ctgcatgttg aggttgagct cggcgatccg atcgacctcg
      301 cggagcacgg tgaccaggaa cttcgcctcg gcgaccagcc cggtcaccgc cagcagcgcc
      361 agcgagccga agtaggtcat gaagtcccag gtcaccttgg cggcggtgat ctcgggccgc
      421 gcgaagcccg ggtactggcc gctccaggtc gagatcaggc ggctggagat cagcaggtag
      481 aggtcgttga agaactggat cgcgaggtcg gcgtccttgc cttccaggga tccggtgacg
      541 aaccgcgtga tcatcgtgtt ggagatcgcg atcatgtccg agccgggcga gtagagcggg
      601 tcgaggaaga ccccggcctc gccggtgatg caccagcgct ccggcgagaa gacctgttcg
      661 cagccgtgcg agtagtgctt gagcaacctg aagtcctgca gctcgccgcg gtggggctcg
      721 agcatctccg cggccagcgg ctcctgctcg gccagccact ccatcagcgg ctcgaagcgg
      781 cgcaggcgct cgaacgggac caggtcgggg tcggcgacca ggccgaagct ggtcgagtcc
      841 gaggcgagcg ggatcaccca gaaccagtag ccgggcccca tgaagtgcac ggtgctctgc
      901 cagcgggtgc cgctgggcac gcgctcgcgc cagcgcggat cctcgctcca cttgtcgatt
      961 tcgatccttc gcccgatccg gaaccaggcg gcgttgcagt ggtgctcgac ctcgacggcg
     1021 agcccgtgct tgcgcttgag gatcgcgccg cgcccgctcg cgtcgacgac ccacgtcgcc
     1081 cgcagctccc cgtcacgggt ggcgaccacg tgcgtgccgt ctccgtcgcg ctcgaccccg
     1141 gtcacgctca cccccccgcg cacctcggcc cccagctctt cggcccgcac cgcgagagcg
     1201 ttctcgaagc ggccgcggtc gagctggtag gtgggcgcgt cgtcggccca gccggccagg
     1261 cccatctcga tccgttgccc gacgtcgtcg cccggcgggg tggcgaggaa gaagcgcagg
     1321 cccatcttgc gcagctgctc gcgctccagg tgctcctcca tcccgcaggt ctcgcggaag
     1381 tagtgggcgc cgatctgcag ggtcgactcg ccgaccttgt gggcggcctc ggggaccggc
     1441 caggcgcgct tctcgagcac cgcgacggag gtcgcggcgc ggcggcgctt cagctggatc
     1501 gccagggtca gcccggcgag cccgccgccg aggacgacga cgtcgaactg ttcaggctgg
     1561 gtgctcatca cgactcctta ctcggtggct gattggctga cggggcgatc gggaaacagg
     1621 cgggcaagcg ggttcggcaa ccaccagttg cggtcgccga gcaccgccag cgccgccggc
     1681 accaggccgc agcggacgag ggtggcgtcg agcagcacgg cgatcgccat gccgacgccg
     1741 agctggcgga agctttcgag gctgctgacc gcggagctga ggaagacgag catgatcacg
     1801 accgccgcga gggtgatcgc gcggccgctc tggcgcagcg cgttggtcac cgccgcgtcg
     1861 ttgccgaccc cgcggtcgta ctcctcgcgc acccgcgaga gcaggaacac ctcgtagtcc
     1921 atcgagagcc cgaagacgat cgcgaagagg aacggcggca cgaaccaggc gagcccgtcg
     1981 gcgccgccga ggtcctggaa gatcagcgtc accaggccga gcgtcgcggc aaccgagagc
     2041 aggttggtca gcaccgcctt cagcggcacg accagcgagc gcagcgcgat cagcaggagc
     2101 acgaaggcga gcgcgcaggc gagcaggatc atcagcccga agcgctcctg gacgcggtcg
     2161 atcaggtcga cgccgagcgc cggttgcccg ccgacgtagg cggcgcccgg gggcgcgacc
     2221 ccggcgagcc gctctcgcac ccggcgcacg gtcgcgtcac ccgccacgct gccgccgctg
     2281 accgccggca gcgcgtcgag ccggacccag ccgtcggcgc cgcgctgagg cggcagcacg
     2341 cgggcgatgc ccgggtcacg ggcgacggcg gcgcgggcgc gggcggcgta agcgggcctg
     2401 accatcactt cgaccggttc gagcagaccg ccgtcgaact cgcggttgac caggtcgctc
     2461 gtctggcgca gctgattgtc ggccggcagc agcccgtcgg tgccggcgaa gacgagcttg
     2521 gtcccgagca ccggcagcgc gcagagcagg aggatcgcga cgctgccgag gaggaccgcc
     2581 cagcggtggc ggagcacgcc gttgccgagc gcccgccaga ggcgcccgcg ctcggcggcc
     2641 cgcacggccg gcttgatcga gaggcggtcg atgcgggggc cg
//
LOCUS       DASRIG010000371         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_184788, whole genome shotgun sequence.
ACCESSION   DASRIG010000371 DASRIG010000000
VERSION     DASRIG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184788"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFB52_09970"
     CDS             complement(<1..492)
                     /locus_tag="VFB52_09970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZO06705.1"
                     /translation="MADQRGWRVWWVKRRWYERNHRRLRRWRLSRHAAAGGFYIRYPV
                     EGEVLEALDEGRLKIGEGTLLEPGCWLTLAPEAEIEIGAGCFLNRNTMIAAQGKVTIG
                     DHTMLANGCFIGDAEHRYDDPDLPITWQGFTSKGPVSIGANCWLGAHVVVASGVNIGE
                     HCVI"
     gene            537..1505
                     /locus_tag="VFB52_09975"
     CDS             537..1505
                     /locus_tag="VFB52_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO06706.1"
                     /translation="MAADTPLRGGIDLGGTKIQTAIVDANGEVLGEARRPTPTDGGPA
                     GVAEQMAEALREAAMAADVEPAQLQGVGVGSPGDADEKTGIVSSAKNLPDWEGSFPLA
                     ETLKEALGTEVRVGNDVQVATEAEFHLGAGREFQSLIGVFWGTGVGGGLVLDGKPWLG
                     RGAAGEIGHVVVKRGGAKCPCGRKGCMEAYAGRSAMEAEARREHEEGHKTDLFKLMEK
                     HGKPRLTSGIWARALDHGDHMAQKLVDRAIEALGTGIASAVNLIDPEAVIIGGGLGIR
                     FGERFMDPLMEEMSKHLFLDQKPPAVRVASLGDLGGAVGASLLFAR"
     gene            1665..>3002
                     /locus_tag="VFB52_09980"
     CDS             1665..>3002
                     /locus_tag="VFB52_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HZO06707.1"
                     /translation="MIPPTPRSLVRLLFSLVALSAFFLPAAVHADDPPTPISAGAGAD
                     WGVKQSFRNYVTGPIANGTISIADGATQNPDGTFHFPVGAGEYDPTTGTTVLGFTGTV
                     HFEGHEGALEVTISDPRIELTPEGSALFADMTSKPNQPGAPVTQYPGVKVAALDLSGK
                     APTVDATTTTWPALPTALAEEAVPAFAGFYGANTALDSFAFGYEGPGGVPQSEDWTPA
                     ATPLWGKALTGTIPTGVVNATFDPVRDRIWVGAYDGKKVVALNDETMASAGIDVSVGH
                     SSRAVAVDRTNGTVYSIDTDIRAVRDSGSGYALDPTAVDSFGGGSNALATDPATGTIY
                     TVVENSLYRYKSPGFSRESFEIPSGYGAVRVTEDGRIFLIPSGAVEVAEVTISGATAT
                     LKPVPGTTAASGATVAADGRIAYVELDYSEYPIVHTTLHVLTPDGSGGWSDHAS"
BASE COUNT          482 a         1085 c         1028 g          407 t
ORIGIN      
        1 gatcacgcag tgctcgccga tgttgacgcc gctggcgacg acgacgtggg cgccgagcca
       61 gcagttggcg ccgatcgaga ccgggccctt cgaggtgaag ccctgccagg tgatcggcag
      121 gtctgggtcg tcgtagcggt gctcggcgtc gccgatgaag cagccgttgg cgagcatcgt
      181 gtgatcgccg atcgtgacct tgccctgggc ggcgatcatc gtgttgcggt tgaggaagca
      241 gccggcgccg atctcgatct cggcctccgg cgcgagggtg agccagcagc ccggctccag
      301 cagcgtgcct tcgccgatct tcagacggcc ctcgtcgagc gcctcgagca cctcgccctc
      361 gaccgggtag cggatataga agccgccggc agcggcgtgg cggctgagcc gccaacggcg
      421 caggcgccgg tggttgcgct cgtaccagcg ccgcttcacc caccagaccc gccacccccg
      481 ttgatcggcc acgcccctag ctttccagag ccgccacgga tagggtgcgc tcgctgatgg
      541 ctgcagacac cccactgcgc ggcggaatcg acctcggcgg caccaagatc cagaccgcga
      601 tcgtcgacgc caacggcgaa gtcctgggcg aggcccggcg gccgacgccg accgacggcg
      661 gcccggccgg ggtcgccgag cagatggcgg aggcgctgcg cgaggcggcg atggcggccg
      721 acgtcgagcc agcgcagctg cagggcgtcg gggtcggctc gcccggcgac gccgacgaga
      781 agaccggcat cgtctcctcg gccaagaact tgccggactg ggaaggcagc ttcccgctgg
      841 cggaaaccct gaaggaagcg ctggggaccg aggtgcgggt cggcaacgac gtccaggtcg
      901 cgaccgaggc cgagttccac ctcggcgcgg ggcgcgagtt tcagagcctg atcggggtct
      961 tctggggaac cggcgtcggc ggcggcctgg tgctcgacgg caagccgtgg ctgggccgcg
     1021 gcgcggccgg tgagatcggc cacgtggtgg tcaagcgcgg cggcgccaag tgcccctgcg
     1081 ggcgcaaggg ctgcatggag gcttacgcgg gccgctcggc gatggaagcc gaggcgcgcc
     1141 gcgagcacga agagggtcac aagaccgacc tcttcaagct gatggagaaa cacggcaaac
     1201 cgcgcctgac cagcgggatc tgggcgcgcg cgctcgacca cggcgaccac atggcccaga
     1261 aactggtcga ccgcgcgatc gaggcgctgg ggaccgggat cgcctccgcg gtcaacctga
     1321 tcgacccgga agcggtgatc atcggcggcg ggctcgggat ccgcttcggc gagcgcttca
     1381 tggacccgct gatggaggag atgagcaaac acctcttcct cgaccagaag ccccccgcgg
     1441 tacgcgtcgc ctcactcggc gacctcggcg gcgccgtcgg cgcctcgttg ctcttcgctc
     1501 gctgacgcca tctggccggc gcggttcggc atctcctcga cgttccacgt agcccgctac
     1561 gcggaagtcg cgtcgctacc gccccgcttg gccagatgac gtcaggctgc gattctgtat
     1621 tagggtggcc taattaggca tccctaataa cgcaaggagc tgagttgatt ccccccaccc
     1681 cccgctcgct cgtccgcctg ctcttctcgc tggtggcact gagcgccttc ttcctgccgg
     1741 ccgcggtcca cgcggacgat ccgcccacgc cgatcagcgc cggagccggc gccgactggg
     1801 gcgtcaagca gtcgttccgc aactacgtga ccgggccgat cgccaacggc acgatctcga
     1861 tcgccgacgg cgccacccag aaccccgacg ggaccttcca cttcccggtc ggcgccggcg
     1921 aatacgaccc gacgaccggg accacggtgc tcggcttcac cggcacggtc cacttcgagg
     1981 gccacgaggg ggcgctggaa gtgacgatct ccgacccgcg gatcgagctg accccggaag
     2041 gctcggcgct gttcgccgac atgacgagca agccgaacca gcccggcgcc ccggtcacgc
     2101 agtaccccgg cgtgaaggtg gccgcgctcg acctcagcgg gaaagccccg accgtcgacg
     2161 cgaccacgac cacctggccg gcgctgccga ccgcgctcgc cgaggaggcg gtgcccgcct
     2221 tcgccggttt ctacggggcc aacaccgcgc tcgactcctt cgccttcggc tacgaaggcc
     2281 ccggcggcgt cccgcagtcg gaggactgga cccccgccgc gaccccgctc tggggcaaag
     2341 cgctgaccgg gacgatcccg accggcgtcg tcaacgcgac cttcgacccg gttcgcgacc
     2401 ggatctgggt cggcgcctac gacggtaaga aagtcgtcgc gctgaacgac gagacgatgg
     2461 cctctgccgg catcgacgtc tcggtcggtc actcgagccg tgccgtcgcg gtcgatcgca
     2521 ccaacggcac cgtgtacagc atcgacaccg acatccgcgc ggttcgcgac agcggttccg
     2581 gctacgcgct cgacccgacc gcggtcgaca gcttcggcgg cggcagcaac gcgctcgcca
     2641 ccgacccggc caccggcacc atctacacgg tcgtcgaaaa cagcctctac cgctacaaaa
     2701 gcccgggctt cagccgcgaa tcgttcgaaa tcccgagcgg ctacggcgcg gtgcgggtga
     2761 ccgaggacgg ccggatcttc ctgatcccga gcggcgccgt cgaggtcgcc gaggtgacga
     2821 tcagcggcgc gaccgcgacc ctgaaaccgg tgccggggac gaccgccgcc agcggcgcca
     2881 cggtcgccgc cgacggacgg atcgcctacg tcgagctcga ctactccgaa tacccgatcg
     2941 tccacaccac cctgcacgtc ttgaccccgg acgggagcgg cggctggagc gaccacgcga
     3001 gc
//
LOCUS       DASRIG010000372         7040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3046287, whole genome shotgun sequence.
ACCESSION   DASRIG010000372 DASRIG010000000
VERSION     DASRIG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3046287"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VFB52_09985"
     CDS             complement(<1..1476)
                     /locus_tag="VFB52_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06708.1"
                     /translation="MAAFLCFTATALAVPPWQAAREIDAGLSEAQTNIILEESGGNTV
                     ERAADDLTGPLRAGLAADAPAELREVETGLAAAERASTARDEAGLAAARGQILAALRR
                     GAMAVAIASAERREPAQAREWLQIRDFRKTTRYTRPGVDGTTAIEELESTQPGSDSDE
                     GISPHEAALLIEKDLLDTYQARLLTNLDEAGQAAERDFDGRFAETTAIVLGYWRAIAA
                     EYGEQRGGAEQAKTERDFERLAAAGANGDEAAFKAARARVEADLKGFTAAPLTPDELV
                     NRASQFLRFIELVPKEYDKGTADGHVTIPFEVQEGVAFMDGVEAAFSDLESELLKRDP
                     GAVDQIEATFAELRTDVDNAQQGKSVAPQEELDAASEKALEEFERIAPDEWMEGGDEA
                     DYELVDISLDQLEAAVGAGRYQEAEQARLAAYGFFEFGPELKLRAFDTGLALEIEGLI
                     WYGANGDKGLATLISDNAPLNEIRETRLDLDEKLDDARAITG"
     gene            complement(1550..2650)
                     /locus_tag="VFB52_09990"
     CDS             complement(1550..2650)
                     /locus_tag="VFB52_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06709.1"
                     /translation="MKLRATLALVLSLALCAALIAGCGDSDDSTSTDATSAEAGTAEL
                     TPVKQYLTDHSAQLEEQVGLLKEQADAYYDLAKSVNFDYDRLLKEHGDEVEEILGTSK
                     DIFVVANPAYEQMEGIVAGVPRLAHYDVDIDAGSDASTPEDAVSFTLTTPDGKELKQP
                     GNLFFLTETALYGTNPDLLAKGVKGDVDGSGTVEFGEGLPDANIYKAALDEFDKQAKE
                     LNKDAQEFEPTPSDAMTSIVIMTPTMSEYFEAWKNSRFIAGEGASELGFVATSRLSDI
                     ADILGGILVTYNGMEPEIAAENPQQAAQTKKELESLVAFASDLRDREADGEKFTAKQA
                     DALGAQAQAQAEAIAGQVTQSAKQLDIELQEG"
     gene            2934..5921
                     /locus_tag="VFB52_09995"
     CDS             2934..5921
                     /locus_tag="VFB52_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZO06710.1"
                     /translation="MLLAPVAAAAPTATGPSGTYAAGDAQINTATPPASKSWTVKNTG
                     TDPVWVVSSTLSGGETDQFQLSGTCATRGEANPLAANETCTVVVGFLPTSTGPKSTTL
                     TTVTNGPTFTTGAITGTGRHLSASVPVDFGDQHVGVASDPVTVTIKNEASEPYPLGTI
                     TTSTNQFVKGADGCASQTLAAGATCDVVLTFLPTTAGAKSGYLTISNHKPHLVPLEGR
                     GTEGAASIAPAVRDFGVQTPSAGSTATTVFTLRNTGNEPLELLDAALLGSGAGAFAIG
                     ADGCSQVDLAGGASCTVEVSFDPTQAGWRAATLAVPVANLPVSPVLARLSGRGSGVTQ
                     GDDDPFGLPGFDGLPLVRYQGDGGDNLGGAIASDAEGCDLNGDGYDDVLAGASLWSKV
                     PAQSSWEGAAYVTFGGPQVGGSDLAATIAGSTIRIEGELERAQTGTGVGCAGDVNGDG
                     VDDLVVGAWAYEYDGRAGGINAPRGVAYVVFGGEDLPNAGPLDLGLLGSRGYKIVAPE
                     SFAYDHLGYQATGVGDLDGDGLDDIALLANTADSPDMTPERTSSGRVYLLPGKAGTAV
                     QDVGTAALTTIVGASPFSTASPWGQMSMISPIGDVNDDGTPDVGIGAYTAVAFGRSTA
                     SGAVFAVSGAERGLTDLADPASSLFAVGGAFAGHRLGIGLAPLGDVSGDGVDDIAIGA
                     DSTAAANSDAAYVVHGAKADPAGTLLDTADLGTRGYRILGAPGSSAGYSVAPAGDVNR
                     DGIGDMLVGGYGAGSNGTAWVVYGPADLTALPANNDGGGSAIGPANTADATRYVALAS
                     LSPEAGSGLAGQTAGERFGRQVAAVGDLDGNGVGDLAVGSDQAVRFERTRAGELTVAL
                     LPGPVPPAPPVEEEPKGGGETPPAGQPPVSDPAPLPLPKLAKRALRLDDSHGVTVRVS
                     CANTAATCRGQVALALAGVIRTRRFTVAPERTVGVRVTLTPRQELALATKGWLRGSVR
                     LTVWQAGEETARTMRVTVRGDKG"
     gene            5918..>7040
                     /locus_tag="VFB52_10000"
     CDS             5918..>7040
                     /locus_tag="VFB52_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06711.1"
                     /translation="MSAGSLRAALAAAVVTAAFATSVAGATAAGPAAPQYDGICRTAS
                     AKAVAGQTVTLALDCRTASRGVLVGRVVGTSHREATAVLTRPRLGVLGTLNRRSGRIA
                     YTANPGAAGKETIRFRVRASDGRRYRGAVVVRIGAAPARPTPPAENPPVPPGEEPPAG
                     DGLPPKLPDAVPGSVASTTRAWVPTAYDSCSKELHDRFSVVGPDGRRYPTWHPPTIVD
                     PASGERCSFGHEHGEDPRGSDLYEWVVGHFAAAGYEQYAGLPFGLAAEALNSWAVATP
                     GTATRSEDHVGYKMDVANDVDLIGPSGAANGVTCDFLTMVHQGSHSPDALSNNAHELL
                     YASRCDDGTEVISSTLSRFGDPGAFQRSCEPGVEIATTDN"
BASE COUNT          932 a         2520 c         2481 g         1107 t
ORIGIN      
        1 gccggtgatc gcgcgcgcgt cgtcgagctt ttcgtcgagg tcgagccgcg tttcgcggat
       61 ctcgttcagc ggggcgttgt cggagatcag cgtggcgagg cccttgtcgc cgtttgcgcc
      121 gtaccagatc aacccctcga tctcgagcgc caagccggtg tcgaaggcgc gcagcttcag
      181 ctccggcccg aactcgaaga agccgtaggc ggcgagccgc gcctgctcgg cctcctggta
      241 gcggccggcg ccgaccgcgg cttcgagctg gtcgagtgag atgtcgacga gttcgtagtc
      301 ggcttcgtcg ccgccttcca tccattcgtc gggcgcgatc cgttcgaatt cctccagcgc
      361 cttttcgctg gccgcgtcga gctcctcctg gggggcgacg gatttgccct gctgggcgtt
      421 gtcgacgtcg gtgcgcagtt cggcgaaggt ggcctcgatc tgatcgaccg cccccgggtc
      481 gcgtttcagc agttcggact cgaggtcgga gaaggcggct tcgaccccgt ccatgaaggc
      541 gaccccctcc tggacctcga aggggatcgt cacgtggcca tcggcggtgc ccttgtcgta
      601 ctccttcggc accagctcga tgaagcggag gaactggctg gctcggttga ccagctcgtc
      661 gggggtcagc ggcgctgcgg tgaagccctt gaggtcggct tcgacccggg ctcgcgcggc
      721 cttgaaggcc gcctcgtcgc cattggcacc ggcggcggcg agccgctcga agtcccgttc
      781 ggtcttcgcc tgctccgcgc cgccgcgctg ctcgccgtac tcggcggcga tcgcccgcca
      841 gtagccgagc acgatcgcgg tcgtttccgc gaagcgcccg tcgaagtcgc gttcggccgc
      901 ctgaccggct tcgtcgaggt tggtcaggag gcgtgcctgg taggtgtcga ggaggtcttt
      961 ttcgatcagc agggccgctt cgtgggggct gatcccctcg tcgctgtcgg acccgggctg
     1021 cgtgctctcc agttcttcga tcgcggtcgt gccatcgacc ccggggcggg tgtagcgggt
     1081 ggtcttgcgg aagtcgcgga tctgcagcca ttcgcgcgcc tgcgccggtt cgcggcgctc
     1141 cgccgaggcg atcgccaccg ccatcgcccc gcgccgcagg gcggcgagga tctgcccgcg
     1201 ggcggccgcg aggccggctt cgtcgcgggc ggtggaggcg cgctcggcgg cggccagccc
     1261 cgtctcgact tcccgcagct cggccggagc gtcggctgcg aggccggcgc ggagcggtcc
     1321 ggtgagatcg tcggcggcac gctcgacggt attgccgccg ctttcttcga ggatgatgtt
     1381 ggtctgggcc tcggagagcc cggcatcgat ttcacgcgcc gcctgccagg gcggaacggc
     1441 gagggcggta gcggtgaagc agaggaaggc cgccaccgcg aaaacgatcg cggtggcggc
     1501 cttcgcggcc ggtcgggtca gtgccttgga ctggaccggt cccgccccct cagccttcct
     1561 ggagttcgat gtcgagctgt ttcgcggact gggtcacctg gccggcgatc gcctcggcct
     1621 gggcctgggc ctgggcaccg agcgcgtcgg cctgcttggc cgtgaatttc tcgccatcgg
     1681 cctcgcggtc gcgcaggtcg ctggcgaaag caaccaggct ttccagctct ttcttcgtct
     1741 gcgccgcctg ctgcgggttc tccgcggcga tctccggctc catcccgttg taggtgacga
     1801 ggatgccgcc gaggatgtcg gcgatgtcgg agaggcgcga ggtggcgacg aagccgagtt
     1861 cggaggcccc ctcaccggcg atgaagcgcg agtttttcca cgcttcgaag tactcgctca
     1921 tggtcggggt catgatcacg atcgaggtca tcgcgtcgga cggcgtcggt tcgaattcct
     1981 gcgcgtcctt gttcagttcc ttcgcctgtt tgtcgaattc gtcgagcgcc gctttgtaga
     2041 tgtttgcgtc cggcaggccc tcgccgaact cgaccgtgcc gctgccgtcg acgtcgcctt
     2101 tgacgccttt ggcgagcagg tcggggttgg tcccgtagag cgcggtctcg gtgaggaaga
     2161 agaggttgcc cggctgtttc agttccttgc cgtcgggcgt ggtcagggtg aaggagaccg
     2221 cgtcttcggg ggttgacgcg tccgagccgg cgtcgatgtc gacgtcgtag tgggccagcc
     2281 gcggcacacc cgcgacgatt ccctccatct gctcgtaggc cgggttggcg acgacgaaga
     2341 tgtccttcga ggtgccgagg atctcctcga cttcatcgcc gtgctctttg agcagccggt
     2401 cgtagtcgaa gttgaccgat ttggcgaggt cgtagtaggc gtccgcctgc tccttgagca
     2461 gcccgacctg ctcttcgagc tgggccgagt gatcggtcag gtactgcttg accggggtca
     2521 gttcggccgt tcccgcctcg gcgctggttg cgtcggtgct ggtgctgtcg tcgctatcgc
     2581 cgcagccggc gattagcgct gcgcacagcg ccagggaaag gaccagcgcc aaagttgctc
     2641 tgagcttcat tcggacgagt gcctcctgag ttaggggagc ctactgaggg ctccctaaat
     2701 gcttggtggt tagggcaccc tacgggaatc cgatcttggg agtcgggatt cggagggtgg
     2761 cgcctggtca gtcgaccgcg gcgcgatgat tagggtagcc taattttgca ttagggtcac
     2821 ctaaccccaa gggaccctgt ccctgattta cctcccccca ccaggaaagg gctcgcatgc
     2881 gctcagtctc gtcccgtcgg tcttttgctg cgctccttac ggtcgcgctc gtcttgctcc
     2941 tcgctcccgt cgccgccgcg gcgcccaccg cgaccgggcc ctcgggcacc tacgctgcgg
     3001 gcgatgccca gatcaacacg gcgaccccgc ccgcgagcaa atcgtggacg gtgaaaaaca
     3061 ccgggaccga tccggtctgg gtggtctcca gcaccctgag cggcggcgaa acggaccagt
     3121 tccagctctc ggggacctgt gcgacgcgcg gcgaagcgaa cccgctcgcc gccaacgaga
     3181 cctgcaccgt cgtcgtcggc ttcctgccga ccagcaccgg gccgaagagc acgacgctga
     3241 ctacggtgac caacgggccg accttcacca ccggcgcgat caccggcacc ggccgccacc
     3301 tctcggcgag cgtgccggtc gacttcggcg atcagcacgt cggcgtcgcc tcagatccgg
     3361 tcacggtgac gatcaaaaac gaggcgagcg aaccgtaccc actgggcacg atcacgacct
     3421 cgaccaacca gttcgtcaag ggggccgacg ggtgcgccag ccagacgctc gccgccggcg
     3481 ccacctgcga cgtcgtcctc accttcctgc cgaccaccgc gggtgcgaag agcggctacc
     3541 tgacgatctc caaccacaag ccgcacctgg ttccgctcga aggccgcggc accgaggggg
     3601 cggcctcgat cgctccggcg gtgcgtgact tcggtgtgca gacgccgagc gcgggctcca
     3661 ccgcgacgac cgtcttcacc ctccgcaaca ccggcaacga gccgctcgag ctcctcgacg
     3721 ccgcgctgct cggctccggc gccggggcct tcgcgatcgg cgccgacggc tgctcgcagg
     3781 tcgacctcgc gggtggtgcc agctgcacgg tcgaggtctc cttcgacccg acccaggccg
     3841 gctggcgcgc cgcgaccctc gcggtgccgg tcgccaacct gccggtctcg ccggtgctcg
     3901 cgcgcctctc cggccgcggc agcggcgtga cccagggcga cgacgacccg ttcggcctgc
     3961 ccggcttcga cgggctgccg ttggtccgct accagggcga cggcggcgac aacctcggcg
     4021 gtgcgatcgc ctccgacgcc gagggctgcg acctcaacgg cgacggttac gacgacgtcc
     4081 tcgccggcgc ctcgctgtgg tcgaaagtgc cggcacagtc ctcctgggag ggggcggcgt
     4141 acgtcacctt cggcgggccg caggtcggcg gctccgacct cgcggcgacg atcgcgggca
     4201 gcaccatccg gatcgaggga gagctggaac gcgcccagac cgggaccggc gtcggctgtg
     4261 ccggtgacgt caacggcgac ggcgtcgacg acctcgtggt cggcgcctgg gcctacgaat
     4321 acgacggtcg cgccggcggg atcaacgccc cgcgcggggt cgcctacgtc gtcttcggcg
     4381 gcgaggacct gccgaacgcc gggccgctcg acctcggcct gctcggctcg cgcggctaca
     4441 agatcgtcgc gccggaatcg ttcgcctacg accacctcgg ctaccaggcg accggggtcg
     4501 gcgacctcga cggcgacggg ctcgacgaca tcgcgctgct cgccaacacc gccgacagcc
     4561 cggacatgac cccggaacgg accagcagtg ggcgcgtcta cctgctgccc ggcaaggccg
     4621 gcaccgcggt ccaggacgtc ggcaccgcgg cgctgaccac gatcgtcggc gcctcgccgt
     4681 tcagcaccgc ctcgccctgg ggccagatgt cgatgatcag cccgatcggc gacgtcaacg
     4741 acgacggcac cccggacgtc gggatcggtg cctacaccgc ggtcgccttc gggcgctcga
     4801 ccgcgagcgg cgccgtcttc gcggtcagcg gcgccgagcg cggcctcacc gacctcgccg
     4861 acccggcgag ctcgctgttc gcggtcggcg gcgccttcgc cggccaccgg ctcgggatcg
     4921 ggctggcgcc gctcggcgac gtcagcggcg atggggtcga cgacatcgcg atcggcgccg
     4981 actcgaccgc ggcggccaat agcgatgccg cttacgtcgt ccacggggcg aaagccgacc
     5041 ccgcgggaac gctgctcgac accgccgacc tcggcacccg cggctaccgg atcctcggcg
     5101 cgccgggctc ctcggctggg tactcggtcg cgccggccgg cgacgtcaac cgcgacggga
     5161 tcggcgacat gctggtcggc ggctatgggg ccgggtccaa cggcaccgcc tgggtcgtct
     5221 acgggcccgc cgacctgacc gcgctgccgg ccaacaacga tggcggcggc tccgcgatcg
     5281 gtccggccaa caccgccgac gcgacccgct acgtggccct ggcatcactc tctcccgagg
     5341 ccggctcggg gctcgccggt cagaccgccg gtgagcgctt cggccgccag gtcgccgcgg
     5401 tcggcgacct cgacggcaac ggcgtcggcg acctcgcggt cggctccgac caggcggtgc
     5461 gcttcgagcg cactcgcgcg ggtgagctga cggtggcgtt gttgccgggc ccggtgccgc
     5521 cggcaccgcc ggtcgaggaa gagccgaaag gcggcggcga gaccccgccg gctgggcagc
     5581 ccccggtgag cgatccggca ccgctgccgc tgccgaagct ggccaagcgg gcgctgaggc
     5641 tcgacgatag ccacggggtc accgtgcggg tctcctgcgc gaacaccgcg gcgacctgcc
     5701 gcggccaggt cgcgctggcg ctcgccgggg tgatccggac tcgccgcttc acggttgcgc
     5761 ccgagcgcac cgtcggggtg cgggtgacgc tgaccccgag gcaggagctg gccctcgcca
     5821 cgaaggggtg gctgcgcggt tcggtgcggc tgaccgtgtg gcaggcgggg gaagagaccg
     5881 cgcggacgat gcgggtgacc gtgcgcgggg acaaggggtg agcgcgggct cgctccgcgc
     5941 ggcgctggcg gcggcagttg tgacggcggc gttcgcgact tcggtcgcgg gcgccaccgc
     6001 cgccggtccc gccgcgcccc aatacgacgg catctgccgg acggcgagcg cgaaggcggt
     6061 cgccgggcag acggtgacgc tggcgctcga ctgccgaacc gcgagccgcg gcgtcctcgt
     6121 cggccgcgtg gtcggcacga gtcaccgaga ggcgaccgcg gtcctcaccc gaccgcgtct
     6181 cggtgtgctc ggaacgctga accggcgcag cggtcgcatc gcctataccg cgaacccggg
     6241 ggccgccggc aaggagacga tccgcttccg cgtccgcgcc agcgacggcc ggcgctaccg
     6301 cggcgccgtg gtggtccgga tcggcgccgc gccggctcgg ccgacgccac cggccgagaa
     6361 cccacccgtg ccgcccggcg aagaaccccc ggccggcgac ggcctgccgc cgaagctgcc
     6421 cgacgcggtc cccggctcgg tcgcttcgac cacccgcgcc tgggtgccga ccgcctacga
     6481 ctcctgctcg aaagagcttc acgaccgctt ctcggtggtc ggccccgacg gtcgccgtta
     6541 cccgacctgg catccgccga cgatcgtcga cccggcgagc ggtgagcgct gcagcttcgg
     6601 tcacgagcac ggtgaggacc cgcgcggctc tgacctctac gagtgggtgg tcggccactt
     6661 cgccgccgcc ggctacgagc agtacgccgg cctgccgttc gggctcgccg ccgaggcgct
     6721 gaactcctgg gcggtcgcca ccccgggcac cgcgacccgc agtgaggacc acgtcggtta
     6781 caagatggac gtcgcgaacg acgtcgatct gatcggtccg agcggcgccg ccaacggcgt
     6841 cacctgcgac ttcctgacca tggtccacca gggcagccac tcgcccgacg cgctctccaa
     6901 caacgcgcac gagctgctct acgcgtcgcg ctgcgacgac ggcaccgaag tgatctcctc
     6961 gaccctcagc cgcttcggcg atcccggcgc cttccagcgc tcctgcgaac ccggggtcga
     7021 gatcgcgacc accgacaacg
//
LOCUS       DASRIG010000373         5166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1493097, whole genome shotgun sequence.
ACCESSION   DASRIG010000373 DASRIG010000000
VERSION     DASRIG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493097"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFB52_10005"
     CDS             <1..546
                     /locus_tag="VFB52_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HZO06712.1"
                     /translation="WALGALSATGKAFPDRIPALARLAGRVASGSRTTDRSDRVFANE
                     RRVRFTEMEYALPRADGPEAVRRVIEWVRGNKYPVFFPIEMRVAAGDDASISPSHERD
                     TAYIAVHQYKGMEWRPYFQAVEAIMNEYSGRPHWGKRHFQTAETLAPRYPAWAEFQAA
                     RDTLDPTRVFTNPYAERVLGQ"
     gene            complement(641..1852)
                     /locus_tag="VFB52_10010"
     CDS             complement(641..1852)
                     /locus_tag="VFB52_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HZO06713.1"
                     /translation="MSVPPTQSHDRYERIFAEVEAPFAFVDLDAMWANADAMLGRAGG
                     KPVRVASKSIRCRALLEEILRRDQGFRGLMTYTLAETLWLAEQGFDDLLLAYPTTDVE
                     ALGELALRSAASPDGAPIVMVDCPEHLDAIESVLGADAAPVRVCIDLDAGWWALGGRV
                     KVGPKRSPVHTVEQAIALAREIEGRPKIELDALMAYEGQIAGVGDTPPGRRLRGAMIR
                     FMQRRSAKELAERRGAVVAALSEFVELEIVNGGGTGSLELTSAEAAVTEVTAGSGFYA
                     PGLFDHYSRFTLTPAAGFALPIVRKPSPKVATALGGGYLASGSGDPARLPSPWLPPGL
                     KLDPEEGAGEVQTPLLGEPAAGLAVGDRAYLRHAKAGELCERFDVLHLVQGDEIVDVV
                     PTYRGEGKTFL"
     gene            1909..1997
                     /locus_tag="VFB52_10015"
     tRNA            1909..1997
                     /locus_tag="VFB52_10015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1943..1945,aa:Ser,seq:gct)
     gene            complement(2016..3056)
                     /locus_tag="VFB52_10020"
     CDS             complement(2016..3056)
                     /locus_tag="VFB52_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HZO06714.1"
                     /translation="MNCPRCQAQGEESRTEVLETRNEGRGGQAALRVRRRRRCPACAY
                     RFTTFELAQLGAVRSPTGLTSPFDEEKLMQSLIAANEDIGIGELELAGAVDAVRAKIE
                     TRPEPVDVAEIDRWLGEALKRHPDLLAAHRDRVRAKHSEGWVRKINGRIEAFSPEKLR
                     AALRDAVHSRINESTLDRLVKTIEERVADANPEPVPTKLIRTWVEEELRATDPFAYLR
                     VASTAPDVDLDSLRRQLLEAIESGLVRKRDSRCEVFSRQKLIISVRKATVKRQTISDE
                     ETRLFADEVAERVRKVGEPVPSDRIGDWVLDWLKRKDAVASMSFLLVFKPPDSPRSLE
                     RELEDWGISHDR"
     gene            3289..4599
                     /locus_tag="VFB52_10025"
     CDS             3289..4599
                     /locus_tag="VFB52_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06715.1"
                     /translation="MRWLVDAFAGIWSGIRGRSRVPRDHDQLWRHGEAEGAELGRQEG
                     AQARGGEHALVDPAPTEAVIKGVVAARIATIGQRVARRYNRLLARADQWKRRGDRRVK
                     RAKVLREARPEVEPRARAIEPFVARTLAYLASFGIVVVILETAGMWTSLEAFEGMIGW
                     LRTPLSIAAGAVLALAGHVIGHLLAAAVEERSLRRGLISGALAVVVIGALAVVWLGVG
                     RDANLRANDSFEQVGKLEATASQLDREANNLLRPTAVPLDGEEVIRPSVADRKAAARL
                     NSQAETVRAKAAELGSDARDERTLDFFAMVQILGLAVGAVGGYFFAGAAPVREFKRLT
                     GKAGREDRRASRRYKRSARWQAKADDVVAKVGRLVDAESAWGAADIARFQETRLITRG
                     YPAGSWRSGTFTKEDLAELLTRTVTPADKNGSRPVRSTDSEGEE"
     gene            4600..>5166
                     /locus_tag="VFB52_10030"
     CDS             4600..>5166
                     /locus_tag="VFB52_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06716.1"
                     /translation="MRKLNVWIAVLAVSLAAAIVSGCGDSTSTACVVVDASHSTRYAL
                     PDYLERLESSVLDAANAGESVAALVATGQPLVESKVETEDFSGLTSSEDIGERNALAQ
                     EFLADVDGSARDASLGTAKPAPGSGIVAAISLVSTRGCDSMLVLSDGLEAADVRMKVD
                     DILSVDGRTALLDKVEQRGRLPRLEGMTV"
BASE COUNT          770 a         1803 c         1816 g          777 t
ORIGIN      
        1 tgggcgctcg gggcgctctc ggcgaccggc aaggcgttcc cggacaggat cccggcgctg
       61 gcccggctcg ccggccgggt cgcgagcggc agccgcacca ccgaccgcag cgaccgcgtc
      121 ttcgccaacg agcgccgcgt ccgcttcacc gagatggagt acgcgctccc ccgcgccgac
      181 ggtccggagg cggtgcggcg ggtgatcgag tgggtgcgcg ggaacaagta cccggtcttc
      241 ttcccgatcg agatgcgggt cgccgccggc gacgacgcct cgatcagccc ctcgcacgaa
      301 cgcgacaccg cctacatcgc cgtgcaccag tacaagggga tggagtggcg cccctacttc
      361 caggcggtcg aggcgatcat gaacgagtac agcggccgcc cccactgggg caagcgccac
      421 ttccagaccg ccgagaccct ggccccccgc tacccggcct gggctgagtt ccaagccgcc
      481 cgcgacaccc tcgacccgac ccgcgtcttc acgaatccat acgccgagcg ggttctcggg
      541 caatagacgg cgaaaaccaa gccgggcaag gacgcaggga gcgccgtgtg ctctgcacac
      601 aagcgaccga ggacgccgcc cggcgccggt ttgcaggcgt ctagaggaag gtcttgccct
      661 cgccgcggta ggtggggact acgtcgacga tctcgtctcc ctggacgagg tgcaggacgt
      721 cgaagcgctc gcagagctcg ccggccttgg cgtggcggag gtaggcgcgg tcgccgaccg
      781 cgaggccggc ggcgggctcg ccgagcagcg gggtctggac ctcgccggcg ccctcctcgg
      841 ggtcgagctt caggccgggc ggcagccagg gggagggcag gcgggccggg tcgccgctgc
      901 cggaggcgag gtagccgccg ccgagcgccg tcgccacctt cggtgagggc ttgcgcacga
      961 tcggcagcgc gaagccggcg gcgggggtga gggtgaagcg gctgtagtgg tcgaagaggc
     1021 cgggggcgta gaagcccgag ccggcggtca cctcggtcac cgcggcctcg gccgaggtca
     1081 gctcgagcga gccggtgccg ccgccgttga cgatctccag ctcgacgaac tcggagagcg
     1141 cggcgacgac cgcgccgcgc cgctcggcca gctccttcgc cgagcgccgc tgcatgaagc
     1201 ggatcatcgc cccgcgcagc cgccgccccg gcggcgtgtc gccgaccccg gcgatctggc
     1261 cctcgtaggc catcagcgcg tcgagttcga tcttcggccg cccctcgatc tcgcgggcca
     1321 gcgcgatcgc ctgctccacc gtgtgcacgg gcgagcgctt cggcccgact ttgacccggc
     1381 cgccgagcgc ccaccagccg gcgtcgaggt cgatgcagac ccggaccggg gcggcgtcgg
     1441 cgccgagcac cgactcgatc gcgtcgaggt gctcggggca gtcgaccatc acgatcggcg
     1501 ccccgtcggg gctggccgcc gagcgcagcg ccagctcgcc gagcgcttcg acgtcggtgg
     1561 tcgggtaggc gagcagcagg tcgtcgaacc cctgctcggc cagccacagg gtctcggcga
     1621 gcgtgtaggt catcagccct cggaagccct ggtcgcggcg caggatctcc tcgagcagcg
     1681 cccggcagcg gatcgacttg ctcgccaccc gcaccggctt gccgccggcg cgccccaaca
     1741 tcgcgtccgc gttcgcccac atcgcgtcga ggtcgacgaa ggcaaacggc gcctcgacct
     1801 cggcaaaaat tcgctcgtag cgatcgtgag actgcgtggg cgggacggac acccacatca
     1861 gcctatcccc gccattcccg gtcagtactt acaatcgagc cttcgcccgg agaggtggcc
     1921 gagtggctga aggcactcgc ctgctaagcg agtagggggc gcaagctctc tcgagggttc
     1981 gaatccctcc ctctccgcct cacgttcggg gccgcctagc gatcgtggct gatgccccag
     2041 tcctccagtt cccgctccag cgagcggggg ctgtcgggcg gcttgaagac cagcaggaag
     2101 ctcatcgagg cgaccgcgtc cttgcgcttc aaccagtcca gcacccagtc tccgatgcga
     2161 tcgctgggga ccggctcgcc gactttgcgc acgcgctccg cgacctcgtc ggcgaagagc
     2221 ctggtttcct cgtcggagat cgtctggcgc tttaccgtgg ccttgcgcac gctgatgatc
     2281 agcttctggc gcgagaagac ttcgcagcgg ctgtcgcgct tgcgcacaag cccgctttcg
     2341 attgcctcca ggagctgccg gcggagcgaa tcgaggtcga cgtcgggggc ggtcgaggcg
     2401 accctcaggt aggcgaaggg atcggtcgcg cgcagttcct cctcgaccca ggtgcggatc
     2461 agcttcgtcg gcaccggttc cgggttggcg tcggcgaccc gctcctcgat tgtcttcacc
     2521 agacggtcga gcgtgctttc gttgatccga ctgtgaacgg cgtcgcggag cgcggcgcgc
     2581 agcttttccg ggctgaacgc ctcaatccgc ccgttgatct tccgcaccca gccctcgcta
     2641 tgcttcgcgc ggacccggtc ccggtgcgcg gcgagcaagt ctgggtggcg cttcagagcc
     2701 tcgccgagcc agcggtcgat ctccgcgacg tcaaccggct cgggccgcgt ctcgatcttc
     2761 gcgcggaccg cgtcgacggc gccggcgagc tccaactcgc cgatcccgat gtcctcgttg
     2821 gctgcgatca ggctctgcat gagcttctcc tcgtcgaaag gagaggtcag cccggtcggg
     2881 ctgcggacgg ctcccagctg ggccagctcg aacgtggtga agcggtaggc gcaggcgggg
     2941 caacgccgcc gtcgccggac gcgcagggct gcctggccgc cacggccctc gttgcgcgtc
     3001 tcgagcacct ccgtgcggct ctcctcgccc tgcgcctggc aacgggggca gttcacggag
     3061 gggactgtac accctgcatg tagagcccgc tcggtaagtc aacgcctgtt cggggccgac
     3121 agaacccaga tataggggga agactcgact agtcccctgt agctagattt cgttaacagg
     3181 atagttacaa ctttggctgg ctgaccaggc agtttcaggg caactcaacg gcgcggcgcc
     3241 gggcgggaga ggttccgggc gcgtcacgaa cagaaggggg acgtcgtcat gagatggctc
     3301 gtcgatgcat tcgccgggat ctggagcggc atacggggcc gcagccgggt gccacgcgac
     3361 catgaccagc tctggcgtca cggcgaggcc gagggcgccg aattgggcag gcaggagggg
     3421 gcgcaggctc gcggtggcga acacgccctg gtcgatccag cgccgaccga ggcggtgatc
     3481 aagggcgtcg tcgccgcccg gatcgccacg atcggccaac gcgtcgcccg tcgctacaac
     3541 cggctgctcg cgcgggcgga tcagtggaag cggcgagggg atcgtcgcgt caaacgcgcc
     3601 aaggtcctac gcgaggcgcg acccgaggtc gagccgcgtg ctcgcgcgat cgaaccgttt
     3661 gtcgcccgca cgctcgccta ccttgcctcc ttcgggatcg tcgtggtgat cctcgagacc
     3721 gccggcatgt ggacctcgct ggaggcgttc gagggcatga tcggctggtt gcggaccccg
     3781 ctcagcatcg ccgccggtgc tgtgctggcg ctggccgggc acgtgatcgg gcacctgctc
     3841 gcggccgcgg tggaagagcg ctccctgcgc cgggggctga tctccggtgc gctcgccgtc
     3901 gtcgtcatcg gcgcgctcgc cgtggtctgg ctcggagtcg gccgcgacgc gaacctgagg
     3961 gcaaacgata gcttcgagca ggtcgggaag ctggaagcga ccgcctcgca gctcgatcgc
     4021 gaagcgaaca atctgctccg gccgaccgcg gtcccgctcg atggcgagga agtcatcagg
     4081 ccctcggtcg ccgatcgcaa agccgccgcg cgtctcaact cgcaggccga gaccgtccgc
     4141 gcgaaagcgg ccgagctcgg gagcgacgcg cgcgatgaac ggacgttgga cttcttcgcg
     4201 atggtccaga tcctcggtct cgcggtcggc gcggtcggcg gctacttctt cgccggcgcc
     4261 gctccggtac gcgagttcaa gcgcctgacc gggaaggccg ggcgcgaaga ccggcgtgcc
     4321 tcgcgccgct acaagcggtc ggccagatgg caggcgaagg ccgacgacgt cgttgccaag
     4381 gtcggtcgtc tagtcgacgc ggagtccgcc tggggtgccg ccgacatcgc ccgcttccag
     4441 gagacccgcc tcatcacccg cggctacccc gccggctcgt ggcggtcggg gaccttcacc
     4501 aaggaggacc tcgcagagct gctcacgcgc acggtcaccc cggctgacaa gaacggaagc
     4561 cggcccgtgc ggtccaccga ctcggaagga gaagagtaga tgcgcaagct caacgtctgg
     4621 atcgcggtct tggccgtgtc cctcgccgcc gccattgtca gtggctgcgg cgactcgacc
     4681 tcgaccgcct gtgtggtggt cgacgcctcg cactcgaccc gttacgcgct gcccgactat
     4741 ctcgagcgcc tcgaatccag cgtgctcgat gctgcaaatg ccggcgaatc cgtcgccgcg
     4801 ctggtcgcca ccgggcaacc gctggtcgag tcgaaggtcg aaacggagga cttctccggg
     4861 ctgaccagca gcgaagatat cggggagcgc aacgcgctcg cccaggaatt cctcgccgac
     4921 gtcgacgggt ccgctcgcga cgcctccctc ggcacggcca agccggctcc cggatcgggg
     4981 atcgtcgccg cgatctcgct cgtttccact cggggttgcg actcgatgct ggtgctctcc
     5041 gacgggctgg aggcggccga cgtgcggatg aaggtcgacg acatcctcag cgtggatgga
     5101 cgtacggcgc tgctcgacaa ggtcgagcag cggggccgcc ttccccggct cgaggggatg
     5161 acggtc
//
LOCUS       DASRIG010000374         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2506079, whole genome shotgun sequence.
ACCESSION   DASRIG010000374 DASRIG010000000
VERSION     DASRIG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506079"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>3727
                     /locus_tag="VFB52_10035"
     CDS             111..>3727
                     /locus_tag="VFB52_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06717.1"
                     /translation="MSVSGGLTGTRLDADPGSWSAEGEISYRYQWQRCDGEGAECEDV
                     EGERASFYLPGEDDADSAFRVVVTAIVAGGNLPVTSAPTAALEATEPEATELPEVFHM
                     EVSGDALEGRSGKWLGIGQVEFESRWLRCDALGEECEEVEGRQDGGYELGEEDVGSTL
                     RFEVTATNDSGNTVAASEETEVIALPSNEAPVLVSPPSIGGDPALGEELTASVGVWSG
                     AVSYRYQWMRCDPDGQECDPIVPTANEAGYETVSDDVGSTIGVVVTALAADGSRAHAS
                     ALSAQTVRAVGAPANSEAPALSGLAEEGESLTASSGSWSGTGPIEYEYRWQRCGPDSC
                     VDIAGATGNTYVLGADDVLQTVRAVVSAENGAGTTATVTAKSAPVDSKAPVLETAPML
                     SGEARKGKTLSATTGAWSGEAPISFDYRWQRCDPAGAACSAISGAEASSYELTGADVG
                     AAVRVLVTATNLSGEGEASSEPTVPVAAAGGPSPKAAPTISGTARTGAELSAGDGEWE
                     GAETFAYQWLRCEPGTGRAECAVIPGAEADEYGPGYDDVGSSLRVLVTATGPAGTAGA
                     VSAGTAAVAQSVSPEVEAAALRKLAPASISGEAVEGESLSADPGEWSSPWAITYSYQW
                     RRCDAAGAGCVNIEEANSPVLTLTGGDVGSTVRVAVTATVGPHSQTSIAKPTAVVAQA
                     KPANLAPPSIFGEAREGSSLFAEVGEWSGSELFTYQWRRCDAEGGECADIEGAEAETY
                     LPTFDDLGATLRVVVTGTNGAGSTPASSAATAVVAPSAPESLYDPEVFGEAFEDGLLE
                     AAPGTWAGTPELEFTYQWQRCDELGEACADIEGATEPTYTADAEDVGSTLQVRVRATN
                     EVGWAEATSSPSELIGPPLVPVFEGPPPTIEGFPYDGRELVAQPGGWSGHEPLEFSYE
                     WQRCDAEGAGCEAIPGATGESYLTTGADLEGTIRVEVTATNTSGSDSETSATTEPLTS
                     AAPLNVEAPALAGEGRVGVELEASAGQWSGSGPLEIAYQWQRCDVECEDIEAATESTY
                     VPGVEDAREEVRVAVTATGPKGSKTDYSETLPILPEASENLSLPTVSGTAVVGETLTA
                     SAGEWSPEPDQYGYQWQRCSISGTGCSDLSGETEATYLLTVGDAGMTLRVVVSAENEG
                     EAEPPSATSILTGVINTETPSNLAQPSLSTTTPVVGQKLTATTGTWIGVPTITYEYEW
                     Q"
BASE COUNT          644 a         1299 c         1314 g          470 t
ORIGIN      
        1 ccctccggat cgcggtgacc gcgaccacga ccggcggcac ggtgaccgtc gcttccgagc
       61 cgagcacggt gatcgaggcc tcgcccgtgc tcaacacctc gccgccgacg gtgagcgtgt
      121 cgggtggcct gaccggtacc cggctcgatg ccgacccggg cagttggagc gccgaaggcg
      181 agatctccta tcgctaccag tggcagcgct gcgacgggga aggggccgag tgcgaggacg
      241 tcgagggcga gcgggcaagc ttctacctac ccggcgagga cgacgcggac tcggccttcc
      301 gcgtggtcgt caccgcgatc gtcgcgggcg gcaaccttcc tgtcacctcg gcgccgacgg
      361 cggccctgga agcaaccgag ccggaagcga ccgagctgcc cgaggtcttc catatggagg
      421 tctccggcga tgcgctcgaa ggccgctcgg gcaaatggct ggggatcggt caggtcgagt
      481 tcgagagccg ctggctgcgc tgcgacgccc tcggcgaaga atgcgaagag gtcgaaggga
      541 ggcaggacgg cggctacgag ctgggcgaag aagacgtcgg ctcgacgctc cgcttcgagg
      601 tgaccgcgac taacgattcc ggcaacaccg tcgccgcctc ggaggagacc gaggtgatcg
      661 cccttccgtc gaacgaagcg ccggtgctgg tctcaccgcc ctcgatcggc ggcgatccgg
      721 cgctgggaga agaactgacc gcttcggtgg gtgtttggag cggagcggtc tcctaccgct
      781 accagtggat gcggtgcgac ccggacggcc aggagtgcga cccgatcgtg cccaccgcca
      841 acgaagccgg atacgaaacc gtctccgacg acgtcggctc gaccatcggg gtcgtggtca
      901 cggcgctggc cgccgacggt tctcgcgctc acgcatcggc cctgagcgcg cagaccgttc
      961 gagccgttgg cgcccccgcc aactcggaag ctcccgccct gtccggcctg gccgaggagg
     1021 gcgaaagcct gaccgcctcc agtggctcct ggtcggggac cgggccaatc gaatacgagt
     1081 accgctggca gcgctgcggc ccggattcgt gcgtcgacat cgccggtgcc accggcaata
     1141 cctatgtcct cggcgccgac gacgtcctgc agaccgtgcg cgcggtggtc agcgccgaga
     1201 acggcgccgg cacgaccgcg acggtcaccg ccaaatccgc cccggtcgac tcgaaagcgc
     1261 cggtcctcga aacggcgccg atgctctccg gcgaagcgcg aaaaggcaaa accctgagcg
     1321 cgacgaccgg cgcctggagc ggcgaagcgc cgatctcctt cgactaccgg tggcagcgct
     1381 gcgaccccgc cggtgcggcc tgctcggcca tatccggggc cgaagcgtcc tcctacgaac
     1441 tgaccggtgc cgacgtcggc gcggccgtgc gcgtgctggt caccgcgacc aatctgtccg
     1501 gagaagggga ggcgagctcc gaaccgaccg tcccggtcgc ggcggccggt ggcccttctc
     1561 ccaaagcggc gccgacgatc tccggcaccg cccggaccgg cgcggagctg agcgccggcg
     1621 acggcgaatg ggaaggcgcg gaaaccttcg cctaccagtg gctgcgctgt gagcccggga
     1681 ccggccgggc cgagtgcgcg gtcatccccg gcgccgaagc cgacgaatac ggccccggct
     1741 acgacgacgt cggttccagc ctgcgcgtgc tcgtcaccgc caccggtccg gccggcaccg
     1801 caggtgccgt ctcggcgggg accgcggcgg tcgcgcagag cgtctctccc gaggtcgaag
     1861 cggcggccct gcgcaagctc gccccggcct cgatcagcgg ggaagccgtg gaaggggagt
     1921 cgctgagcgc cgatcccggc gaatggagct caccctgggc gatcacctac tcctaccagt
     1981 ggcgccgctg cgatgccgcc ggcgcggggt gcgtcaacat cgaagaagcc aacagtccgg
     2041 tcctcaccct caccggcggc gacgtcggct ccaccgtgcg ggtggcggtg accgcgaccg
     2101 tgggcccgca cagccaaacg tcgatcgcca aaccgacggc ggtggtggcc caggcgaaac
     2161 cggccaacct cgcacccccg tcgatcttcg gggaagcgag agaaggcagc tccctgttcg
     2221 ccgaagtggg ggaatggagc ggcagcgaac tcttcaccta ccagtggcgg cgctgcgacg
     2281 cggaaggcgg cgaatgcgcc gacatcgaag gcgccgaggc ggaaacgtat ctgcccacgt
     2341 tcgatgacct gggcgcgacc ctgcgcgtgg tggtaaccgg caccaacggg gccggctcca
     2401 cccccgcgag ctcggccgcg acggcggtgg tcgctccctc cgcgccggaa agcctgtacg
     2461 acccggaagt gttcggcgag gcgttcgagg acggcctgct ggaggcggcc ccgggaacct
     2521 gggccggtac gccggaactg gaattcacct accagtggca acgctgcgac gaactgggcg
     2581 aagcctgcgc tgacatcgaa ggggcgaccg aaccgaccta caccgccgac gccgaagacg
     2641 tcggctcgac gctgcaggtg cgggtgcggg cgacgaacga agtcgggtgg gcggaggcga
     2701 cctcctcgcc gagcgaattg atcggcccgc ctctcgtacc ggtcttcgaa ggcccgccgc
     2761 cgacgatcga aggcttcccc tacgacggcc gcgagctggt cgcccagccc ggtggctgga
     2821 gcggccatga gccgctggaa ttcagctacg agtggcagcg ctgcgatgcg gaaggcgccg
     2881 gctgcgaagc gatccccggc gccaccgggg agagctacct gaccaccggc gcggacctcg
     2941 aaggcacgat cagggtcgag gtcaccgcca cgaacaccag cggctcggac agcgaaacgt
     3001 cggcgacgac cgaaccgctg accagcgccg ccccgttgaa cgtcgaggcc ccggcgctcg
     3061 ccggcgaagg cagggtcggg gtcgagctcg aggcgtctgc cgggcagtgg tccgggagcg
     3121 gaccgctgga aatcgcctac cagtggcagc gctgcgacgt cgaatgcgaa gacatcgaag
     3181 cagcgaccga atccacctac gtgcccgggg tagaagacgc gcgcgaggag gtgcgggtcg
     3241 cggtcaccgc aaccggcccg aaaggcagca aaaccgacta ctccgaaacg ctgccgatcc
     3301 tgcccgaagc gtcggagaac ctgagcctgc cgacggtctc cgggacggcg gtcgtcggcg
     3361 aaaccctcac ggcctcggcc ggcgaatggt ctccggaacc cgatcagtac gggtaccagt
     3421 ggcagcgttg ctcgatcagc gggaccggct gctccgacct ttcgggcgag acggaagcga
     3481 cgtacctgct gaccgtgggc gacgccggga tgacgctgcg ggtcgtggtc agcgccgaaa
     3541 acgaaggcga agcggaacct ccgtcggcga cctcgatcct caccggcgtg atcaacaccg
     3601 aaactccctc gaacctggcc cagcccagcc tcagcaccac cacgccggtc gtcggccaga
     3661 aactgacggc gaccacgggc acctggatcg gagttcccac gatcacctac gagtacgagt
     3721 ggcagca
//
LOCUS       DASRIG010000375         7226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2795216, whole genome shotgun sequence.
ACCESSION   DASRIG010000375 DASRIG010000000
VERSION     DASRIG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795216"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFB52_10040"
     CDS             <1..753
                     /locus_tag="VFB52_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO06718.1"
                     /translation="PVLEIMFGDFLALSMDSLINQATKYWFLTGEKQGCPLTIRSVVG
                     AGGRFGAIHSQSPVSWFLGVPGLKIATPSNPGDARTLMKAAIRDDNPVLFFEHKRLYS
                     VKGELNDDPVELGGARVVRPGSDLTIVSAMTGVGIALAAAEQLAADGIDAEVIDMRVL
                     RPFDYQTPVESLAKTNRLLVLEEGPRTGGWSATALAEISEHALHHVDDIWRITTADLP
                     IPFSPSLEDAFLPSAESVATAVRNRLGAPVGS"
     gene            complement(801..1433)
                     /locus_tag="VFB52_10045"
     CDS             complement(801..1433)
                     /locus_tag="VFB52_10045"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06719.1"
                     /translation="MGQRLRSAREEQGLSLREVARRLDISASALSQIETGKSRPSVKT
                     LYAIVSELGLSMDELFAERGDERAAPAPSAPSAAPNGAPAEAPPVVQRADSRSSLELD
                     SGVTWDRLTAAHDTNVDFLFTTYAPGSSSSGSEKLVRHNGREYGLILSGELELSVGFE
                     KYRLGPGDSCSFDSNEPHRLANPGDEPTTAIWFVIGRRQSDPRQISFDGD"
     gene            complement(1490..2638)
                     /locus_tag="VFB52_10050"
     CDS             complement(1490..2638)
                     /locus_tag="VFB52_10050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO06720.1"
                     /translation="MPVRATKRGDERTPLEGRHDVLICGASFAGLAIARELAGSGADV
                     LIVDRYEIGERQTSACGIPTEWLRRLGLMDSECQRFDTLVMHTPHGTSRYKLPWTFST
                     FDYRELCALLWADCDARFETAKVNGRAPSPNGGGEIAVETDRGVLTSPLVVDALGWRR
                     VLASGDGYQPPDAPLSRGLEVHPAGVHDDLEIWIDRKYVPAGYGWSFPARDELRIGVG
                     SFDPRFHVKDTTVLLTEDLGKEPNEYQGNWIPHKLRKATEGGVFFAGDSAGHCLPLSA
                     EGIRTALYFGIALGRELRGVVEGRQSREQAAATYAEFNDSHEWKFKWMLRVQKLVPRV
                     PPRVLAPLIRLMGNKRFVNWSFNHYLAIAPPEFVQEPETASKPAPVPA"
     gene            2716..4266
                     /gene="rpsA"
                     /locus_tag="VFB52_10055"
     CDS             2716..4266
                     /gene="rpsA"
                     /locus_tag="VFB52_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZO06721.1"
                     /translation="MTPAPSATPVDGSNGLLLQIDGKIVPNYAATMVSFDEGDVVNGK
                     VVRIDKDEVLVDIGYKAEGVIPSNELSIRKSVDPAEEVELGEEIDALVLTKEDAEGRL
                     ILSKKRARFEKAWRRIEIAADSGEPVEGSVIEVVKGGLILDLGVRGFLPASLVDIRRV
                     HNLDEFMGQTLECKVIELNRSRNNVVLSRRAVLEEERKEVREQILGRLEPGQVVEGKI
                     SNIVDFGAFVDLDGIDGLIHISELSWSHVNHPSEVVAIGDTVRIKVLDIDRDRQRISL
                     GLKQTQEDPWQRVISTHRPGDVLEGQVTKVVAFGAFVEILPGVEGLVHISELADHHVE
                     NPSEVVEPGSTLNVKILEIDEERRRLSLSIKRVEGQNMPMQDLGQQIAAATEAPAADE
                     AAATEEAPAAEVAEAAPEAEAPAVEAEAEVAAVEAEPAVEEAAPEAPAEEAAPEEPVA
                     EAEPAAEAEPVAEAEPVAEANGETSEAGEEAHEGESVADRPDSPDDVDEASGENADDA
                     ESEASSDS"
     gene            4273..4851
                     /gene="coaE"
                     /locus_tag="VFB52_10060"
     CDS             4273..4851
                     /gene="coaE"
                     /locus_tag="VFB52_10060"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934343.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZO06722.1"
                     /translation="MPLKIGLTGGIAAGKSEALAAFGRLGAATLSSDAVVHELLESEP
                     LRGRLVERWGPEVAPAEGVDRAKIGGIVFADPEELAWLEAQIHPLVAERMVEWLTELP
                     AGTELAVVEVPLLFEGGREAVFDTTVAVVAGEDVRRTRAEARGHTLVDEREARQLTQA
                     EKAERAEHVVANDGSVEDLEEALSALIDKLRG"
     gene            4848..5426
                     /locus_tag="VFB52_10065"
     CDS             4848..5426
                     /locus_tag="VFB52_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZO06723.1"
                     /translation="MSRQVRRRRRFALGSAAALVGVVVGLLVVGGTFDKAIQELTLPL
                     RHEDIIRQQSREKGVDASLIAAVIYSESKFVDQTSHAEARGLMQITPDAADYIAKQSG
                     ATDFSLKDLGDPEINIRYGTFLLAEFLERYDGDEAAALAAYNAGPGNADEWGGADLTV
                     EDIDFPETRAYVEEVLEKRHEYRDEYGQELGY"
     gene            5426..6796
                     /locus_tag="VFB52_10070"
     CDS             5426..6796
                     /locus_tag="VFB52_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO06724.1"
                     /translation="MNDAARPRVAALALAVGIVLADSSIVVLALPEIYRELDTSVAGV
                     TWVLISFNLVMALAAVPAAHVARRFGPGLTAAIGLAVFAGAGLACGLASELSTLIVAR
                     CVQALGGAAAVTAALELLPATVGSEKRATAVWASAGATGAALGPAVGGLLTELISWQS
                     IFLIQVPISLAAAVPILTVAKHEMATRVIESELRPVGRPHLAANLALAMVSAAIAAAL
                     FLLVLLLIEGWRLTPIGAAIVVSVMPLAALLGGRLGPRVPSAKARAAAGAILVSGGLG
                     GLALLPKAEVALTVPPQILIGIGLSLVLSALTETALGGRAPQAIHGGWTISARHAGVV
                     IGLLALTPIFTADIAQQREDAIDAGTAVLLDSKVTPLLKLELAGKISDQLDGEKGKVP
                     TIGPAFKPLPSDPEERAQVETLRDELQDQLDRGATHAFSPSFGLAALIGLLALIPIGL
                     SRRVDL"
     gene            6793..>7226
                     /locus_tag="VFB52_10075"
     CDS             6793..>7226
                     /locus_tag="VFB52_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06725.1"
                     /translation="MSEGRLNSDFRSQLLVGGAIVASLVLVVVYLAAGGSSYTPEQVQ
                     DPCKHREWTDPGTLQEVAQQFSLSALDGAACELGVSREELARALPDEEARQRFLDEYD
                     IGTEEFAKAIRAGLLRAIEDAEDAGALSPLVAGPLRATIEDIP"
BASE COUNT         1068 a         2581 c         2513 g         1064 t
ORIGIN      
        1 cccgtgctcg agatcatgtt cggcgacttc ctcgcgctgt cgatggacag cctgatcaac
       61 caggcgacca agtactggtt cctgacgggc gagaagcagg gctgcccgct gacgatccgc
      121 tcggtggtgg gcgccggcgg ccgcttcggc gcgatccact cgcagagccc ggtctcctgg
      181 ttcctcggcg tgcccggcct gaagatcgcc acgccctcga accccggcga cgcccgcacc
      241 ctgatgaagg cggcgatccg cgacgacaac ccggtcctct tcttcgagca caagcgcctc
      301 tactcggtca agggcgagct caacgacgac ccggtcgagc tcggcggcgc ccgcgtggtc
      361 cggcccggca gcgacctgac gatcgtcagc gcgatgaccg gcgtcggcat cgccctcgcc
      421 gcggccgagc agctggctgc cgacgggatc gacgccgagg tgatcgacat gcgcgtcctg
      481 cgcccgttcg actaccagac cccggtcgag tccctggcca agaccaaccg cctgctcgtg
      541 ctcgaggagg gcccgcgaac cggcggctgg tcggccaccg ccctcgccga gatctccgag
      601 cacgcgctcc accacgtcga cgacatctgg cgaatcacca ccgccgacct gccgatcccg
      661 ttcagcccga gcctcgagga cgccttccta cccagcgccg aatccgtggc caccgccgtc
      721 cgcaaccgcc tcggcgcgcc ggtcggttcg tgagtctgct ctagaccccg tatatcgggg
      781 tcaaatgcag actcgcgggc tcagtccccg tcgaagctga tctggcgcgg gtcgctctgg
      841 cggcggccga tcacgaacca gatcgcggtg gtcggctcgt cgccggggtt ggcgagccgg
      901 tgcggttcgt tggagtcgaa cgagcaggag tcgccggggc cgagccggta tttctcgaac
      961 ccgaccgaga gctccagctc gccgctcagg atcaggccgt actcgcggcc gttgtggcgg
     1021 accagtttct cgctgccgct ggatgagctg ccgggcgcgt aggtggtgaa gaggaagtcg
     1081 acgttggtgt cgtgggcggc cgtgaggcgg tcccaggtga cgccggagtc cagctccagc
     1141 gaggagcgcg agtcggcgcg ctggaccacc ggcggcgcct cggcgggcgc gccgtttggc
     1201 gccgcggacg gggcggaggg ggcgggagcg gcgcgctcgt cgccgcgctc ggcgaacagc
     1261 tcgtccatcg agaggccgag ttcggagacg atcgcgtaga gggtcttgac cgagggccgt
     1321 gacttgccgg tctcgatctg ggagagggcg ctggcggaga tgtcgaggcg gcgggcgacc
     1381 tcacgcaggc tcaggccctg ttcctcgcgg gcgctgcgca gccgctggcc cacctgggcc
     1441 gagtcatctc cctttatccc ctccgaagct tgtttagcca caccgaacat tatgcgggaa
     1501 ccggcgccgg cttgcttgcc gtctccggtt cctgcacgaa ttcgggcgga gctatcgcga
     1561 ggtagtggtt gaacgaccag ttgacgaagc gtttgttgcc catcagccgg atcaacggtg
     1621 cgagcacccg gggcggaacc cggggaacca gcttctgcac ccgcagcatc cacttgaact
     1681 tccactcgtg ggagtcgttg aactcggcgt aggtggcggc cgcctgctcg cggctctgcc
     1741 tgccctcgac cacgccgcgc agctcgcgcc ccagcgcgat cccgaagtag agcgccgtgc
     1801 ggatcccctc ggcgcttagc ggcaggcagt ggccggccga gtcgccggcg aagaagacgc
     1861 cgccttcggt cgctttgcgc agtttgtgcg ggatccagtt gccctggtac tcgttcggct
     1921 ccttgccgag gtcctcggtc agcagcacgg tcgtgtcctt gacgtggaag cgggggtcga
     1981 aggagccgac cccgatccgc agctcgtcgc gggccgggaa cgaccagccg tagccggccg
     2041 gcacgtactt gcggtcgatc cagatctcga ggtcgtcgtg gacgccggcc gggtgtacct
     2101 cgaggccgcg cgagagcggt gcgtcgggcg gctggtagcc gtcgccggag gcgagcacgc
     2161 ggcgccagcc gagtgcgtcg acgaccagtg gggaggtgag cacgccgcgg tcggtttcga
     2221 ccgcgatctc accgccgccg ttcggcgagg gcgcgcggcc gttcaccttg gcggtctcga
     2281 agcgggcgtc gcagtcggcc cagagcagcg cgcagagctc gcggtagtcg aaggtcgaga
     2341 aggtccaggg cagcttgtag cgcgaggtcc cgtgcggcgt gtgcatcacc agggtgtcga
     2401 agcgctggca ctcggagtcc atcagtccga gccggcgcag ccactcggtc gggatcccgc
     2461 aggccgaggt ctgacgctcg ccgatctcgt agcggtcgac gatcagcacg tcggcgccgg
     2521 agcccgccag ctcacgcgcg atcgcgaggc cggcgaagct ggccccacat ataaggacgt
     2581 catggcggcc ttccaggggc gtacgctcgt ccccgcgctt ggtggctctg accggcatct
     2641 gtcggcagcg tatctaggcg ggacctcggc cgcgcgtttc tgccatcatg cgcgtccgtg
     2701 tccgccaccg agaccatgac ccctgcccca agcgccacgc ccgtagacgg ttcgaacggg
     2761 ctgctgctgc agatcgacgg caaaatcgtc cccaactacg cggcgacgat ggtgtcgttc
     2821 gacgagggcg acgtggtcaa cggcaaagtc gtccgcatcg acaaagacga ggtgctcgtc
     2881 gacatcggct acaaggccga gggcgtcatc ccgtccaacg agctttcgat ccgcaagtcg
     2941 gtcgacccgg ccgaagaggt cgagctgggc gaggagatcg acgccctggt ccttactaag
     3001 gaggacgccg agggccgcct gatcctctcg aagaagcgcg cccgcttcga gaaagcctgg
     3061 cgccgcatcg agatcgccgc cgactcgggc gaaccggtcg aaggcagcgt gatcgaggtg
     3121 gtcaagggcg gtctgatcct cgacctcggg gtgcgcggct tcctgcccgc ctcgctggtc
     3181 gacatccgcc gcgtccacaa cctcgacgag ttcatgggcc agacgctcga gtgcaaagtg
     3241 atcgagctga accgctcccg caacaacgtc gtgctctcgc gccgcgccgt cctcgaggag
     3301 gagcgcaagg aggtccgcga gcagatcctc ggccgtctcg agccgggcca ggtcgtggaa
     3361 ggcaagatct ccaacatcgt cgacttcggc gccttcgtcg acctcgacgg gatcgacggc
     3421 ctgatccaca tttccgagct ttcctggagc cacgtcaacc atccctccga agttgtcgcg
     3481 atcggcgaca cggtgcggat caaagtgctc gacatcgacc gcgaccgcca gcgcatctcc
     3541 ctcggtctga agcagaccca ggaggacccg tggcagcgcg tgatcagcac ccaccgcccg
     3601 ggcgacgtgc tcgagggcca ggtgaccaag gtcgtcgcct tcggcgcctt cgtcgaaatc
     3661 ctgcccggcg tcgagggtct ggtccacatc tccgagcttg ccgaccacca cgtcgagaac
     3721 ccgagcgaag tggtcgagcc cggctccacc ctgaacgtga agatcctcga gatcgacgaa
     3781 gagcggcgcc gcctctcgct cagcatcaag cgggtcgagg gccagaacat gccgatgcag
     3841 gacctcgggc agcagatcgc tgccgccacc gaggctcccg ccgccgacga ggccgctgcc
     3901 accgaggagg ctccggccgc cgaggtagcc gaggccgctc ccgaggccga ggcccccgcg
     3961 gtcgaggccg aggctgaggt cgctgccgtc gaggcagagc ccgccgttga ggaggccgct
     4021 cccgaggcac ccgccgagga ggccgctccc gaggagccgg tcgccgaggc cgagcccgcc
     4081 gccgaggccg agccggttgc tgaggccgag ccggtcgccg aggcgaacgg tgagacttct
     4141 gaggccggcg aggaggcgca cgagggtgaa tcggtcgccg accggcccga ctcgcccgat
     4201 gacgtcgacg aggcttccgg cgagaacgcc gacgacgccg agtccgaggc gtcctccgac
     4261 tcctagacgg cgatgccgct gaagatcggc ctgaccggcg gtatcgcggc cggtaagtcc
     4321 gaggcactgg ccgccttcgg ccgcctgggt gcggccaccc tctccagcga cgcggttgtc
     4381 cacgagctgc tcgagtccga gccgctgcgc ggcaggctgg tcgagcgctg gggtcccgag
     4441 gtggcgccgg cggagggcgt cgatcgcgcc aagatcggcg ggatcgtctt cgccgacccc
     4501 gaggagctcg cctggctcga ggcccagatt cacccgctgg ttgccgagcg catggtcgag
     4561 tggctgaccg agctgccggc tggaaccgag ctcgccgtgg tcgaggtgcc gctgctgttc
     4621 gagggcggcc gcgaggcggt cttcgacacc accgttgccg tcgtcgccgg cgaggacgtg
     4681 cgccgcaccc gcgccgaggc ccgcggccac accctcgtcg acgagcgcga ggcacgccag
     4741 ctgacccagg ccgagaaggc cgagcgggcc gagcacgtgg tcgccaacga cggatctgtg
     4801 gaggacctcg aagaggcgct gtccgcgctg atcgacaagc tgcggggatg agtcgccagg
     4861 tccgacggcg gcgccgcttc gcgctcggct ccgccgccgc actggttggc gtggtggtcg
     4921 gtctgctcgt cgtcggcggc accttcgaca aggcgatcca ggagctgacc ttgccactgc
     4981 gccacgagga catcattcgc cagcagtcgc gcgaaaaggg cgtcgacgcc tcgctgatcg
     5041 ccgcggtcat ctactcggag tcgaagttcg tcgaccagac ctcgcacgcg gaagcgcgcg
     5101 ggctgatgca gatcaccccc gacgcggccg actacatcgc gaaacagagc ggcgccaccg
     5161 acttcagcct caaggacctc ggcgaccccg aaatcaacat ccgctacggg accttcctgc
     5221 tcgccgaatt cctcgaacgc tacgacgggg acgaggcggc ggccctggcc gcctacaacg
     5281 ccgggcccgg caacgccgac gagtggggcg gggcggacct caccgtcgag gacatcgact
     5341 tcccggaaac gcgcgcctac gttgaagagg tgctggagaa gcgccacgag tatcgcgacg
     5401 agtacgggca ggagctgggc tactgatgaa cgacgctgcc cgcccccgcg tcgccgcgct
     5461 cgctctcgcc gtcggcatcg tcctcgccga ctcctcgatc gtcgtcctcg cgctgcccga
     5521 gatctaccgc gaactcgaca ccagcgtcgc cggcgtcacc tgggtgctga tctccttcaa
     5581 cctggtgatg gcgctggccg cggtcccggc cgcgcacgtc gcccgccgct tcggtcccgg
     5641 gctgacggcg gcgatcgggc tggcggtctt cgccggcgcc gggctcgcct gcggcctcgc
     5701 ctccgagctc tcgaccctga tcgtcgcccg ctgcgtgcag gcgctcggcg gcgccgccgc
     5761 ggtcaccgcc gcgctcgagc tgttgccggc gacggtcggc tcggagaagc gcgccaccgc
     5821 ggtctgggcc agcgccggcg cgaccggggc ggcgctcggg ccggcggtcg gcggcctgct
     5881 caccgagctg atctcctggc agtcgatctt cctgatccag gtgccgatct cgctcgctgc
     5941 cgcggtcccg atcctgaccg tcgccaaaca cgagatggcg acccgggtga tcgagagcga
     6001 gctgcgcccg gtcgggcgcc cccacctcgc cgccaacctc gccctggcga tggtctcggc
     6061 ggcgatcgcg gcggcgctct tcctgctcgt cctgctgctg atcgagggct ggcgcctgac
     6121 cccgatcggc gccgcgatcg tcgtctcggt gatgccgctg gcggcgctgc tgggggggcg
     6181 cctcggaccg cgcgtgccct cggcgaaggc gcgcgccgcc gccggcgcga tcctcgtctc
     6241 cggcgggctc ggcggccttg cgctcctgcc gaaggccgag gtcgcgctga ccgtgccgcc
     6301 gcagatcctg atcgggatcg ggctctcgct ggtgctctcg gccctgaccg agaccgcgct
     6361 cggcggccgc gccccgcagg caatccacgg cggctggacg atctccgccc gccacgccgg
     6421 ggtcgtgatc ggcctgctgg cgctgacccc gatcttcacc gccgacatcg cccagcagcg
     6481 cgaagacgcg atcgacgccg gcaccgcggt cctgctcgac tcgaaggtga cgccgctgct
     6541 gaagctggag ctggccggca agatcagcga ccagctcgac ggcgagaagg gcaaagtgcc
     6601 gaccattggt cccgccttca agccgctgcc gagcgacccg gaggagcgcg cccaggtcga
     6661 aaccctgcgc gacgaactgc aggaccagct cgaccgtggc gccacgcacg cgttcagccc
     6721 ctcgttcggg ctcgccgccc tgatcggcct gctcgcgctg atcccgatcg gcctctcgcg
     6781 gagggttgac ctgtgagcga gggccgcttg aactccgact tccgctccca gctcctggtc
     6841 ggcggcgcga tcgtcgcctc gctcgtgctc gtcgtcgtct acctcgccgc cggcggttcc
     6901 tcctacacgc cggagcaggt ccaggatccg tgcaaacacc gcgagtggac cgatcccggg
     6961 acgctccagg aggtcgccca gcagttctcg ctctcggcgc tcgacggcgc cgcctgcgaa
     7021 ctcggcgtca gccgcgagga gctggcccgc gccctgcccg acgaagaggc gcgccagcgc
     7081 ttcctcgacg agtacgacat cggcaccgaa gagttcgcga aggcgatccg ggccggcctc
     7141 ctgcgggcga tcgaggacgc cgaagacgcc ggcgcgctca gcccgctggt cgccgggccg
     7201 ctgcgggcga cgatcgagga catccc
//
LOCUS       DASRIG010000376         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3288248, whole genome shotgun sequence.
ACCESSION   DASRIG010000376 DASRIG010000000
VERSION     DASRIG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288248"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /gene="kdpB"
                     /locus_tag="VFB52_10080"
     CDS             <1..1705
                     /gene="kdpB"
                     /locus_tag="VFB52_10080"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZO06726.1"
                     /translation="PAPELQRGDVVIVSAGEIIPADGEVVEGVGSVDESAITGESAPV
                     IRESGGDRSAVTGGTRVLSDRIVVEVTQEPGQSFLDKMIALVEGAERRKTPNEIALNI
                     LLAGLTLIFLLVVVTLRPFAEFSGTDASIVTLVALLVALIPTTIGALLSAIGIAGMDR
                     LVRHNVLALSGRAVEASGDVDVLLLDKTGTITLGNREATQFIPMPGIEESELAEAAQL
                     SSLADETPEGRSIVVLAKEYGIRGRELQSHEAHFVPFSAETRMSGVDVDGNQYRKGAG
                     DAIIAFVEQQGGRPPADLQEHLDLVGREGGTPLAVCRDNHVLGVVYLKDIVKEGMTAR
                     FDELRRMGISTVMVTGDNKLTAAKIAEESGVDDFLPEATPEQKLALIRSHQEEGRMVA
                     MAGDGTNDAPALAQSDVGVAMNTGTQAAREAGNMVDLDSNPTKLIEIVEIGKQLLITR
                     GALTTFSIANDVAKYFAILPAIFAFTYAANPGERGPLDDLNVMSLGTPLSAILSAIVF
                     NALVIPLLVPLALRGVRYRPIGATALLRRNMLVYGLGGLIVPFIGIKLIDLLIHNVLG
                     A"
     gene            1711..2238
                     /locus_tag="VFB52_10085"
     CDS             1711..2238
                     /locus_tag="VFB52_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06727.1"
                     /translation="MAPTVLAHLAHHGVHAPARRVGPHRVSAADRIAVALTSFDLDRR
                     LASGEDPDSSPRLALRGRRLQRRRHRDELAKRINLALAAAEDRPWSGGSSVPLDREEV
                     IRCRVQLLDLAERVRCGPVTVRGLAMVGQMLRDGSSPLFSLREGPPRKPGALARYLAA
                     AAAALDDEGARPPSR"
     gene            2294..3406
                     /locus_tag="VFB52_10090"
     CDS             2294..3406
                     /locus_tag="VFB52_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZO06728.1"
                     /translation="MAAGVGKTYRMLQEGHAEQDGGRDVVVGYMEPHRRPDTSALALG
                     LETLPRRRLSYRGAELEEMDVEALLARAPELALIDELAHTNAPGAGHGKRYEDVRDVL
                     AAGIDVFSTVNVQHLESLNDQVAELTGTRVRETIPDAVLGEADEVVLIDLTPEALIAR
                     LQAGKIYPGENVEAALNNFFTVENLLALREVALRQVAQEVEAKRLTVEEPLGTREERV
                     ADRTPQAVGERLLALVRPQPSSQRLVRRAWRSAQRLGTDLDLLWVKPPGQPIEGEIER
                     QVTALRQLASVLGANFLVEENDSLVDGAARVVRERGSTYIMVGESEPRRGLTRLREPL
                     PQRLMRATPPGVDVRIVAHRGTKEGSGNGPAGEGRR"
     gene            3403..3984
                     /locus_tag="VFB52_10095"
     CDS             3403..3984
                     /locus_tag="VFB52_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO06729.1"
                     /translation="MIAIEHVLIVVALLAGLAGGYLLARSRWRGRRPRSDERLHHILL
                     PFTGTEISRRAVDAALRLARAEDAALMPAYLAAVPMRLPLDCAIPAEAATAMAMLEAI
                     EQRAAAQGVQVDARIERGRSYRHALERLLGREDFDRVVVPAGTNGADGLSGEDLVWLL
                     RSAPAEVLILRPAPEDTRTVGPHVSRPAGVVSV"
     gene            3981..4475
                     /locus_tag="VFB52_10100"
     CDS             3981..4475
                     /locus_tag="VFB52_10100"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZO06730.1"
                     /translation="MSAVETYWSTYESPIGELTLVARGGRLREVRFPGGGNGLTAELC
                     QPDEFANAVRQLDEYFVGERRAFDLQIELLGSPFQRAVWTALREIPFGETVSYGEIAK
                     RLELDGPEAARDVGTAVGQTPTPIVIPCHRVIGANGDLTGYGGGLDRKRALLDLEASQ
                     LSLL"
     gene            complement(4472..4984)
                     /locus_tag="VFB52_10105"
     CDS             complement(4472..4984)
                     /locus_tag="VFB52_10105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZO06731.1"
                     /translation="MVQLVRGTPELMEAAIAGDAALAEVLGHPVTAGWVSFTEALAPT
                     RDEIVANPERAEWGVRFFLAGEPAELVGWGGFKGPPANGVVELGYEIAPSRQRQGLAS
                     GAVAAMLAEAFAAAEVTMMIAHTLPEKNPSNRLLENVGFVHEGVADDGEEIAWRFGLT
                     RSDWELRRGD"
     gene            complement(5043..5471)
                     /locus_tag="VFB52_10110"
     CDS             complement(5043..5471)
                     /locus_tag="VFB52_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06732.1"
                     /translation="MKLHLKFAMVLGALVLAMAPALALAAPSQTGQENGNGPKYAPEK
                     PDKPETPGPKDPLPEQAKAYGVYCKGESKKHVKGEKGTDFSRCVNAMAKAAQSEKKTA
                     REACKGLSKKHVKGEKGTAYSRCVSAAAKLKKDQREAANA"
BASE COUNT          764 a         2035 c         1937 g          838 t
ORIGIN      
        1 gccggcgccc gagctgcagc gcggcgacgt cgtcatagtc tcggcgggcg agatcatccc
       61 cgcggacggc gaggtcgtcg agggcgtcgg ctcggtcgac gagtcggcga tcaccggcga
      121 gtcggcgccc gtgatccgcg agtccggcgg cgatcgatcc gcagtcaccg gcggcacccg
      181 ggtgctctcg gaccggatcg tggtcgaggt gacccaggag cccgggcaga gcttcctcga
      241 caagatgatc gccctggtcg agggagccga gcggcgcaag acgccgaacg agatcgccct
      301 caacatcctg ctcgccggcc tcacgctgat cttcctgctg gtcgtcgtca ccctgcgccc
      361 gttcgccgag ttctccggca ccgacgcctc gatcgtcacc ctggtcgcgc tgctggtagc
      421 gctgatcccg accacgatcg gcgccctgct ctcggcgatc gggatcgccg gcatggaccg
      481 cctggtccgc cacaacgtgc tcgccctctc cgggcgcgcg gtcgaggcct ccggcgacgt
      541 cgacgtgctg ctgctcgaca agaccggcac gatcacgctc ggcaaccgcg aggcgacgca
      601 gttcatcccg atgcccggga tcgaggagtc cgagctggcc gaggcggccc agctctcctc
      661 gctggccgac gagaccccgg aggggcgctc gatcgtcgtg ctcgccaagg aatacgggat
      721 ccgcgggcgc gagctccagt ctcacgaggc gcacttcgtc cccttctcgg ccgaaacccg
      781 gatgagcggg gtggacgtcg acggcaacca gtaccgcaag ggcgcgggcg acgcgatcat
      841 cgccttcgtc gaacagcagg ggggccggcc gcccgccgac ctgcaggagc atctcgacct
      901 ggtcggccgc gagggcggca cgccgctggc ggtctgccgc gacaaccacg tgctcggcgt
      961 cgtctacctg aaggacatcg tcaaggaggg gatgaccgcc cgcttcgacg agctgcggcg
     1021 gatgggcatc tcaaccgtga tggtgaccgg cgacaacaaa ctgaccgcgg cgaagatcgc
     1081 cgaagagtcg ggggtcgacg acttcctgcc ggaagcgacg ccggagcaga agctggcgct
     1141 gatccgctcc caccaggagg aggggcggat ggtggcgatg gccggtgacg ggaccaacga
     1201 cgcgcctgcc ctcgcacagt cggacgtcgg cgtggcgatg aacaccggca cccaggcggc
     1261 gcgcgaggcc ggcaacatgg tcgacctcga ctcgaatccc acgaagctga tcgagatcgt
     1321 cgagatcggc aagcagctgc tgatcacccg cggcgccctc accacctttt cgatcgccaa
     1381 cgacgtcgcc aagtacttcg cgatcctgcc ggcgatcttc gccttcacct acgccgccaa
     1441 ccccggcgaa cgcggaccgc tcgacgacct caacgtgatg tcgttgggca cgccgctctc
     1501 ggcgatcctc tcggcgatcg tcttcaacgc cctggtgatc ccgctgctgg tgccgctggc
     1561 cctgcgcggc gtgcgctacc ggccgatcgg cgcgaccgcg ctgctgcgcc gcaacatgct
     1621 ggtctacggc ctcggcggcc taatcgtccc cttcatcggg atcaagctga tcgacctgct
     1681 gatccacaac gtcctggggg cgtagccgga ttggcgccca cggtcctcgc ccacctcgcc
     1741 caccacggcg tgcacgcacc ggcgcggcgc gtggggccac atcgggtcag cgccgccgac
     1801 cggatcgccg tcgccctgac cagcttcgac ctcgaccggc ggctggcgag cggcgaggac
     1861 cccgactcga gcccacgcct ggcgctgcgt ggccggcggc tgcaacgccg gcgtcaccgc
     1921 gacgagctgg cgaagagaat caacctggcc ctcgccgccg ccgaagaccg accctggtcc
     1981 ggcggctcca gcgtgcccct ggaccgcgaa gaggtgatcc gctgtcgcgt ccagctcctg
     2041 gacctggccg agcgggttcg ctgcggtccg gtgacggtcc ggggcttggc tatggtcggt
     2101 cagatgctgc gcgacgggtc gagtccgctt ttctcattgc gcgagggtcc accgcggaaa
     2161 cccggtgccc tggcccgcta cctggcggcg gctgctgccg cgctggacga tgaaggcgcc
     2221 cggcccccga gccgctgagg aagccggcgg ttcggcgccc gctgggcgag gcaggctgaa
     2281 agtcttcctc gggatggcgg ccggggtcgg caagacctac cggatgttgc aggaaggcca
     2341 cgccgagcag gacggcggcc gcgacgtcgt cgtcggctac atggagcccc accgccggcc
     2401 ggacacctcg gcgttggcgc tcgggctgga gaccctgccc cggcggcggc tctcctaccg
     2461 cggcgccgag ctcgaggaga tggacgtcga ggcgctgctg gcccgcgctc ccgagttggc
     2521 gctgatcgac gagctcgccc acaccaacgc ccccggcgcc gggcacggca agcgctacga
     2581 agacgtgcgc gacgtgctgg ctgccgggat cgacgtcttc tcgaccgtca acgtccagca
     2641 cctggagtcg ttgaacgacc aggtcgccga gctgaccggg acccgggtgc gggagacgat
     2701 tcccgacgcg gtgctcggcg aggccgacga ggtggtgctg atcgacctca cgcccgaggc
     2761 gctgatcgcg cggctgcagg cgggcaagat ctacccgggc gagaacgtcg aggcggccct
     2821 gaacaacttc ttcacggtcg agaacctgct ggccctgcgc gaggtcgccc tgcgccaggt
     2881 tgcccaggag gtcgaggcga agcggttgac cgtcgaggag ccgctcggca cccgcgagga
     2941 gcgggtcgcc gaccggacgc cgcaggcggt cggcgagcgg ctgctcgcgc tggtgcgccc
     3001 gcagccctcc tcgcagcgac tggtgcgccg cgcctggcgc tccgcccagc ggctcgggac
     3061 cgacctcgac ctgctctggg tgaagccgcc gggacagccg atcgagggcg agatcgagcg
     3121 gcaggtgacg gcgctgcgcc agctcgcctc ggtgctcggc gccaacttcc tggtcgagga
     3181 gaacgacagc ctcgtcgacg gcgccgcccg ggtcgtgcgc gagcgcggct cgacctacat
     3241 catggtcggt gagtccgaac cgcggcgcgg cctgacgcga ttgcgcgaac cgctgccgca
     3301 gcggctgatg cgggcgaccc cgcccggggt cgacgtgcgg atcgtcgccc accgcgggac
     3361 caaggagggc agcggcaacg gcccggcggg agaggggcgg cggtgatcgc gatcgagcac
     3421 gtgctgatcg tcgtcgccct gctcgccggc ctcgccggcg gctatctgct cgctcgctcg
     3481 cgctggcgcg ggcgccggcc gcgctccgac gagcgcttgc accacatcct gctgcccttc
     3541 accggcaccg agatctcgcg ccgcgccgtc gacgccgcgc tgcgcctcgc ccgcgccgag
     3601 gacgcggcgc tgatgcccgc ctatctcgcc gcggtgccga tgcgcctgcc gctcgactgc
     3661 gcgatcccgg ccgaggcggc caccgcgatg gcgatgctgg aggcgatcga gcagcgcgcc
     3721 gccgcccagg gcgtccaggt cgacgcgcgc atcgagcgcg gccgctccta ccgccacgcg
     3781 ctcgagcgcc tgctcggccg cgaggacttc gaccgcgtcg tcgtccccgc cggcaccaac
     3841 ggcgccgacg gcctcagcgg cgaggacctc gtctggcttc tgcgcagcgc ccccgccgag
     3901 gtcctgatcc tgcgcccggc ccccgaggac acccgcacgg tcggccctca cgtttcccgt
     3961 cccgccggcg ttgtatctgt atgagcgctg tggagaccta ctggtcgacc tatgagagcc
     4021 cgatcggcga gctgaccctg gttgcgcggg gtggccggct gcgcgaggtt cgcttcccgg
     4081 gcggcggcaa cggcctcacc gcggagctgt gccagccgga cgagttcgcc aacgcggtgc
     4141 gccagctcga cgagtacttc gtcggcgagc ggcgcgcctt cgacctgcag atcgagctgc
     4201 tcggctcacc cttccagcgt gccgtctgga cggcgctgcg cgagatcccc ttcggcgaga
     4261 ccgtctccta cggcgagatc gccaagcgac tcgagctcga cggcccggaa gcggcccgcg
     4321 acgtcggcac cgcggtcggg cagacgccga ccccgatcgt catcccctgc catcgcgtga
     4381 tcggcgccaa cggcgacctc accggctacg gcggcggcct cgaccgcaag cgggccctgc
     4441 tcgacctcga ggcgagccag ctgagcttgc tctagtcgcc ccggcggagt tcccagtcgg
     4501 accgggtgag cccgaagcgc caggcgatct cttcgccgtc gtcggcgacg ccttcgtgca
     4561 cgaagccgac gttttcgagc agccggttgg aggggttctt ttccggcagc gtgtgggcga
     4621 tcatcatcgt cacctccgcc gcggcgaacg cttccgccag catcgccgcc accgcgccgc
     4681 tggccaggcc ctggcgctgc cggctcggcg cgatctcgta gccgagctcg accaccccgt
     4741 tggccggcgg tcccttgaaa ccgccccagc cgaccagctc cgccggctcg ccggcgagga
     4801 agaaacgcac gccccactcg gcccgctcgg ggttggcgac gatttcgtcg cgggtcgggg
     4861 ccagcgcctc ggtgaagctg acccagccgg ccgtgaccgg atgcccgagg acctcggcca
     4921 gcgccgcgtc gccggcgatc gccgcctcca tcagctcagg ggtaccgcga accagctgga
     4981 ccattgcccc agcatcgcac gggcgggcga cgatgctggg gctcggtcct gagtggaaat
     5041 ccttaggcgt ttgccgcctc gcgttggtcc ttcttcaact tcgcggcggc gctcacgcag
     5101 cgtgagtagg cggttccctt ctcgcctttc acgtgcttct tgctcagccc tttgcaggcc
     5161 tcgcgggccg tcttcttctc gctctgggcg gccttcgcca tcgcgttcac gcagcggctg
     5221 aagtccgtgc ccttctcgcc tttgacgtgt ttcttgctct cgcccttgca gtagacgccg
     5281 taggccttcg cctgctcggg cagcgggtcc ttcgggcccg gggtctcggg cttgtccggc
     5341 ttttcgggcg cgtacttggg gccgttgccg ttttcctggc ccgtctgcga gggcgcagca
     5401 agggcaaggg cgggggccat ggccaagaca agtgcgccga ggaccatcgc gaacttcagg
     5461 tgcaacttca tcgtgcctcc ttcggtcttc gatctgcgct tccagggctg aaacgcagga
     5521 aggaggcttt tgtcaggtgc tagttctcct gctgtccggg ggcggcggcg cttt
//
LOCUS       DASRIG010000377         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2257438, whole genome shotgun sequence.
ACCESSION   DASRIG010000377 DASRIG010000000
VERSION     DASRIG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257438"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..356)
                     /locus_tag="VFB52_10115"
     CDS             complement(63..356)
                     /locus_tag="VFB52_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06733.1"
                     /translation="MSSELPYDPTRDPRWEAAVNEMEWVRSSDETVSKQGPCPRCGHE
                     VTFVDEARPASEERAGSELGMFEECNCGVVHPGTPEGRIGCGANGRIQRSQGA"
     gene            complement(413..1120)
                     /locus_tag="VFB52_10120"
     CDS             complement(413..1120)
                     /locus_tag="VFB52_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06734.1"
                     /translation="MLPALGWGCLAASSLVIGAVLAFAREWTRPQVGVVLAFGAGALI
                     SAVSFELWEEGSAIGGLGWTGLGLAIGALTYYACDSRIDRRGGRGGEADTGKALALGA
                     FLDGIPEQLVLGIGLAGGSGVSVALLVAIFASNLPEAIGSARGMVESGGSRAAVLRLW
                     AGVAGACALATVVGYAIADAASGGLHAAVNGFAAGALLVMLIDSMIPVASRDAGRVAG
                     LVTALGFAVAALLSSLS"
     gene            1172..1636
                     /locus_tag="VFB52_10125"
     CDS             1172..1636
                     /locus_tag="VFB52_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06735.1"
                     /translation="MPIFAAFRVRKPRAPGRPASYYEVAGIALDQEMERLCRLPVFAG
                     GPLAKRRPELRVRRASRKPNRLGFAVPREFRLSVTAYPGIGPGDVLETLVHELTHLHV
                     GRADEAHAWHGPAFKHALGEAMYEAYGLMVPVPRSTRHGFYAEAIEELLVAA"
     gene            1681..4077
                     /locus_tag="VFB52_10130"
     CDS             1681..4077
                     /locus_tag="VFB52_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06736.1"
                     /translation="MRRVSSTFSLAALALLCFCLVAQAGTAGGGLDRAFGVRGFGGGE
                     LGPHHVTTSFGAVAEQPDGSLLVTRDETRRRYLADGTLDPGFPPQKVEPSPLEVTLPD
                     GRRLVRGKASGTIARLNPDGTPDPSFHGGESEPTASYFPRYFVPLPSGQILVAGEANR
                     LVGKSGLEYRTGVSRLNADGTLDRSFGDAGLVFVPTDATGYLLDLVGEPDGGGILIGY
                     GGAVRLTPAGALDPSFGSGGVAAVPGGSTVSVNLLPGGGFAIAGTVRDGSTPAADLFV
                     AQFGASGQLDTSFAAGKGIAVADLGGTDTAGAVRWEDDGSVLVGGAATARNPACEAVE
                     SCVEQPVLVRFGAAGVLDPALGGDGVVRLDPLAGPAGRFGTGGVAAISPRGAGGYFLG
                     GSAAPERTTAFLAAIGADGALDPAFGSAGIVRETRSVPSSEGSGPVVVAADGTILVGG
                     STTAGYGKPAAVFHYTRDGKLDPAYGGEPGFARLSGIDRVLDIAVARSGAAIVLGSGG
                     ELTRVTADGRIDPGFGSGLPVRFERPKDAFNSIALLPDDRVLVAGTANWHGDRSRMLV
                     ARFLPDGRLDRSFGRGGFAAVGCARRGRCAVNQIVAQPDGRILLAGRIQKSGAKAQYM
                     QKPSRLAVARLRADGRPDRSFSGDGVVNLRLGYHATAREIAWSRRGILVAGRVGARSG
                     ERETVLLRLRFGGSLDRGFGRGGIVRRPEPSETVALLSTPRRIFVVFEEGPRAVLGFD
                     PAGHLDYSLPSRRLRRGSKWSIYPEAALQGRKPILVWSMFKGPNPTSPSQIELARLNG
                     "
     gene            complement(4078..4150)
                     /locus_tag="VFB52_10135"
     tRNA            complement(4078..4150)
                     /locus_tag="VFB52_10135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4115..4117),aa:Thr,seq:cgt)
     gene            4464..4537
                     /locus_tag="VFB52_10140"
     tRNA            4464..4537
                     /locus_tag="VFB52_10140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4498..4500,aa:Arg,seq:acg)
BASE COUNT          744 a         1855 c         1706 g          764 t
ORIGIN      
        1 ctcgcgcgga cagggcgacg ccaaccgcac cacctactgg tgcccccgct gtcagcccta
       61 ggctaggcgc cctgggaccg ctggatccgg ccgttcgccc cgcacccgat ccgcccttcg
      121 ggcgtgcccg ggtggacgac cccgcagttg cattcctcga acatccccag ctcgcttccc
      181 gcccgttcct cgctcgccgg gcgggcttcg tcgacgaagg tgacctcgtg cccgcagcgc
      241 gggcagggcc cctgcttgga gacggtctcg tcgctgctgc ggacccactc catttcgttc
      301 acggccgcct cccagcgggg atcacgggtc gggtcgtagg gcagctcgga gctcatcgag
      361 cgccgcatcg taactactga ttgaacgacg ttcgttaact aaagatttgt cttcacgaga
      421 gcgaggagag cagggccgcg accgcgaacc cgagcgcggt caccaggccg gccacccggc
      481 cggcgtcgcg gctggcgacg gggatcatcg agtcgatcag catcaccagc agcgcccccg
      541 cggcgaaccc gttgacggcg gcgtgcaggc caccggaggc agcgtcggcg atcgcgtatc
      601 cgaccaccgt ggcgagcgcg caggcgccgg caactccggc ccagaggcgc agcaccgcgg
      661 cgcgcgagcc gccgctttcg accatgccgc gggccgagcc gatcgcctcc ggcaggttgg
      721 aggcaaagat cgctaccagc agggcgacgc tgacccccga cccgccggcg agcccgatcc
      781 cgagcaccag ctgctcgggg atgccgtcga ggaaggcgcc cagcgccagc gccttgccgg
      841 tgtccgcctc gccgccgcgt ccgccgcggc ggtcgatccg cgagtcgcag gcgtagtagg
      901 tgagcgcccc gatcgccagg cccagtccgg tccagccgag gccgccgatt gccgacccct
      961 cctcccagag ctcgaagctg accgcgctga tcagcgcccc agctccgaag gcgagcacga
     1021 cgccgacctg tggccgcgtc cactcgcgcg cgaacgccag caccgccccg atcaccaggg
     1081 aggaggcggc gagacagccc cagccgagag caggcagcat cgcccaaagc tagatctcga
     1141 cgcagtcgga gaccgcggct atgctcagcc gatgccgatc tttgccgcct tccgcgtccg
     1201 caagccgcgg gcgccgggcc ggccggcgag ctactacgag gtcgcgggga ttgcgctcga
     1261 ccaggagatg gagcgcctct gccgcctccc cgtcttcgcc ggcggcccgc tcgccaagcg
     1321 ccgcccggag ctgcgcgttc gccgcgccag ccgtaagccg aaccgcctcg gcttcgccgt
     1381 cccccgcgag ttccgcctct cggtcaccgc ctatcccggc atcggccccg gcgacgtgct
     1441 cgagaccctc gtccatgagc tgacccacct gcacgtcggc cgcgccgacg aagcccacgc
     1501 ctggcacggc ccggctttca agcacgcgct cggcgaagcg atgtacgagg cctacggcct
     1561 gatggtcccg gttccccgca gcacccgcca cggcttttac gccgaggcga tcgaagagct
     1621 gctggtcgcc gcttagcgac cgcaccgcta cttccgtccc gcttccgtgt cgttgcgggc
     1681 atgcgcaggg tctcgtccac cttctcgctc gcggccctcg cgctgctctg cttctgcctg
     1741 gtcgcgcagg ccggcaccgc gggcggcggt ctcgaccgcg ccttcggggt acgcgggttc
     1801 ggcggcggcg agctcgggcc gcaccacgtc acgacgagct tcggagcggt tgccgaacag
     1861 cccgacgggt cgctgctggt cacccgtgac gaaacccgcc gccgctacct ggccgacggc
     1921 actctcgatc ccggcttccc gccgcagaag gtcgaaccga gcccgctcga agtcaccctg
     1981 cccgacggca ggcggctggt caggggcaag gcgagcggca ccatcgcccg gctgaacccc
     2041 gacggcacgc ccgacccgag cttccacggc ggcgaaagcg agccgaccgc cagctacttc
     2101 ccgcggtact tcgtgcccct gccctcgggc cagatcctcg ttgccggcga ggcgaaccgg
     2161 ctggtcggca agagtgggct cgaataccgc accggcgtga gccggctgaa cgcggacggc
     2221 acgctcgacc gcagcttcgg ggacgcgggg ctggtcttcg tgccgaccga cgccaccggc
     2281 tacctgctcg acctggtcgg ggagccagac ggcggcggga ttttgatcgg ctacggggga
     2341 gcggtccggc tcacgccggc gggggcgctc gacccgagct tcggcagcgg cggcgtggcg
     2401 gccgtgccag gggggagcac ggtcagcgtg aatctgctgc cggggggcgg gttcgccatc
     2461 gccggaaccg tgcgggacgg ctcgaccccc gctgccgacc tcttcgtcgc ccagttcggc
     2521 gccagcgggc agctcgacac ctctttcgcc gccggcaagg ggatcgcggt cgccgacctc
     2581 ggtgggaccg acacggccgg tgcggtgcgc tgggaagacg acgggtcggt cctggtcggc
     2641 ggcgcggcaa cggcccgcaa tcccgcgtgc gaagcggtcg agagctgcgt ggagcaaccg
     2701 gtgctggtcc gcttcggcgc cgccggcgta ctcgaccccg ccctgggggg cgacggcgtg
     2761 gtcaggctcg acccgctggc gggacccgcg ggccgattcg gcaccggggg agtggcggcg
     2821 atctccccgc gcggcgccgg cggctacttc ctcggcggtt ccgccgcgcc cgaacggacc
     2881 accgccttcc tcgccgcgat cggtgccgac ggcgccctcg acccggcctt cggcagcgcc
     2941 gggatcgtgc gcgaaacccg ctccgtgccc agcagcgagg gcagcggacc ggtcgtcgtc
     3001 gctgcggacg gcacgatcct cgtcggcggc tcgaccaccg cgggctacgg caaaccggcg
     3061 gcggtgttcc actacacccg cgacggcaag ctcgaccccg cctacggcgg cgaaccgggc
     3121 ttcgcccggc tctccgggat cgaccgcgtg ctcgacatcg cggttgcgag gtccggtgcg
     3181 gcgatcgtgc tcggcagcgg cggcgagctg acccgggtga cggcggacgg caggatcgat
     3241 cccggcttcg gctccggcct gccggtccgg ttcgaacgac cgaaggacgc cttcaactcg
     3301 atcgccctgc tgccggacga cagggtcctg gtcgcgggaa cggccaactg gcacggcgac
     3361 cgctcgcgga tgttggttgc ccgcttcctc cccgatggcc gcctcgaccg ctctttcggc
     3421 cgcgggggct tcgcggccgt cggctgcgcc cgacgtggtc gctgcgccgt caaccagatc
     3481 gtggcccagc cggacgggcg catcctgctg gccgggcgga tccagaagtc cggcgccaaa
     3541 gcccagtaca tgcagaagcc gagccggctg gcagtggcgc gcctgcgcgc cgacggccgc
     3601 ccggaccgca gcttcagcgg cgacggagtc gtcaacctgc gactcggcta ccacgccacg
     3661 gctcgcgaga tcgcttggag ccggcgaggc atcctggtcg ccggtcgggt gggcgcgcgc
     3721 agcggagagc gcgagaccgt gctgctgcga ctgcggttcg gtgggagcct cgaccgcggc
     3781 ttcggccgcg gcgggatcgt acgccgcccc gaaccttccg aaacggtggc gctgctttcc
     3841 acgccgagac gcatcttcgt cgtcttcgag gaggggccgc gcgccgtgct cggcttcgac
     3901 cccgccggcc acctcgacta ctcgctcccc tcgcggcgcc tccgaagggg ctcgaagtgg
     3961 agcatctacc ccgaggccgc gctgcagggc cgcaagccga tcctcgtctg gtcgatgttc
     4021 aagggaccga accccacttc gccgtcgcag atcgaactgg cgcgcctcaa cggctgaagc
     4081 cgacgagcgg acttgaaccg ctgacccctt cattacgaat gaagtgctct accaactgag
     4141 ctacgccggc ataccgcatg catactagga aaccggcgga tttgaccccc gtttcccaga
     4201 tggtctgtcc ctcaacgtcc ctcaaattct gcttcttggg ttcaaaatac ggctctccta
     4261 ttgagcagag atctggccta tagagcggat cttcagccga tcccgagtag cagcctttca
     4321 cccgaatgaa agtgccctac caaccgagta tcgccggcat tcggcttccg ttctagggaa
     4381 cggcgtttag gttccgtctt gccgaggccc gtccctcatt ttctctcaag gccctgttct
     4441 tgagggcgga atcgtcattg ggcgtgcccg tagctcagcc ggatagagcg tcggtctacg
     4501 aaaccgaagg tcacaggttc gaatcctgtc gggcgcgtat gtttggcttt gctagagccg
     4561 tttaccgaca cccttctggc ccttaagcgt tcggtgtgac agattttctg aaccgaaatt
     4621 ttttagagag ttcgtgcctg gggccgcggg ggccgctcat tcagaggggc ccgtttccct
     4681 aatccacctc atctcgggcg cctcctcagc tcgaagactc cccatcctga aacgcaccac
     4741 ccatgacgct agtagcgacg tggcgaggga cggaacccac gccacgcttc gagatgaaga
     4801 cgatggactg cgggggcgct taagctcgta gatgactggt acatgccggg gcgccagacc
     4861 ctgtccgtgc gtgggtcgtc gaatggctcg atagaagtga cggcgagccg aagatgccgc
     4921 ggtccagcct tgacgtcgca gcggagctcg cccccgaccc tcggcggcgt ggacccctgc
     4981 gcacggcgta gtgccgggcg gcgcgcgccg cctctcagat tcccgagaag cgagagcgga
     5041 gccaattgct tcatgcggcg acatgtgat
//
LOCUS       DASRIG010000378         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_955403, whole genome shotgun sequence.
ACCESSION   DASRIG010000378 DASRIG010000000
VERSION     DASRIG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955403"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFB52_10145"
     CDS             complement(<1..463)
                     /locus_tag="VFB52_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZO06737.1"
                     /translation="MPATATFEDRVRAWEDEFLSAYATRSYPANRRRAEENSPMRTPF
                     QRDRDRIVHSKSFRRLKHKTQVFVAPEGDHYRTRLTHTLEACGIARTVACALGLNEDL
                     TEAIGLGHDLGHPPFGHAGEETLDKALQGSCGIGFKHNEHSLRVVDVLERDG"
     gene            complement(473..1201)
                     /gene="recO"
                     /locus_tag="VFB52_10150"
     CDS             complement(473..1201)
                     /gene="recO"
                     /locus_tag="VFB52_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZO06738.1"
                     /translation="MPGNTVKTEAIVLRSIRYGEADRILHLYSVDRGRIGAIAKGARK
                     PKSRFGGRLEPFFRLDLVLHEGRGDLLTVTGVTTVDGYPRLRSSGPALSAGARACDAV
                     LRLLDSTEPNQAAYNLLCRYLTLLDDPEAADKAAGLEVALSFRLKLALIAGFAPELAS
                     CARCGEADHLVGFSGAAGGVVCTSCEAGSFELSEEAHAFMVAALAKPLIEAPAAEPLA
                     LRQVERAVGETLEHHAHVQLRAAA"
     gene            complement(1206..2108)
                     /gene="mutM"
                     /locus_tag="VFB52_10155"
     CDS             complement(1206..2108)
                     /gene="mutM"
                     /locus_tag="VFB52_10155"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZO06739.1"
                     /translation="MPELPEVETVARQLEPELEGHRIEELEVLDARWSRPVPPEKLGA
                     AVSGSTIESLGRRGKYLLLGLDGERTLVMHLRMTGNLVLVEGEDVLDPSEGRRLYEGE
                     RSTSARHLRARFLLDDGREVWFTDPRRFGEAFLLPNDGLETRFAGKVGVEPLSPEFTP
                     EAFGEMAAGRVVPLKSFLLDQKGVAGVGNIYADEALYRARLHPLSPAGSMKPEHLLAL
                     RDAVVASLEAGIEAGGSSIDDYRDGRGEKGTMQEKFLVHTREGEPCLSCGGAIVRIVV
                     SGRSTYFCPTCQVRLRRRPRRRAK"
BASE COUNT          307 a          728 c          846 g          315 t
ORIGIN      
        1 cgccgtcgcg ctcgagcacg tcgaccaccc gcagcgagtg ctcgttgtgc ttgaagccga
       61 tcccgcagct cccctgcagc gccttgtcga gggtttcctc gccggcgtgg ccgaagggcg
      121 ggtggccgag gtcgtggccg agcccgatcg cctcggtcag gtcctcgttg aggccgagcg
      181 cgcaggcgac ggtgcgggcg atcccacagg cctccagcgt gtgggtgaga cgggtgcggt
      241 agtggtcgcc ctccggagcg acgaagacct gggtcttgtg cttgagccgg cggaaggact
      301 tggagtggac gatgcggtcg cggtcgcgct ggaacggcgt ccgcatcggg ctgttctcct
      361 ccgcccttcg gcggttggct ggataggagc gggtcgcgta ggcggagagg aactcgtcct
      421 cccaggcgcg gacgcggtcc tcgaaggtgg cggtggcggg cacccagcga tcttaagccg
      481 cggcgcgcag ctgcacgtgc gcgtggtgct ccagggtctc gccaactgcg cgctcgacct
      541 ggcgcagggc gagcggctcg gccgcggggg cctcgatcag cggcttggcc agcgccgcga
      601 ccatgaaggc gtgcgcttct tcagagagtt cgaatgagcc ggcctcgcag ctggtgcaga
      661 ccacgccgcc ggcggcgccg gagaagccga ccaggtggtc cgcctcgccg cagcgggcgc
      721 aggaggcaag ctcgggggca aagccggcga tcagcgccag cttgaggcgg aaggagagcg
      781 ccacttcgag cccggcggcc ttgtcggccg cctccggatc gtcgagcagg gtgaggtagc
      841 ggcagagcag gttgtaggcg gcctggttgg gctcggtcga gtcgagcagg cgcagcacgg
      901 cgtcgcaggc gcgggcgccg gcggagagcg ccgggccgga tgagcgcagg cgcgggtagc
      961 cgtcgacggt ggtgacgccg gtcaccgtga gcaggtcccc gcgcccttcg tggagcacga
     1021 ggtcgagccg gaagaacggc tccaggcggc caccgaagcg ggatttcggc ttgcgggccc
     1081 ccttcgcgat cgccccgatc cgaccgcgat cgactgaata gagatggagg atgcgatccg
     1141 cctccccgta gcggatgctg cgcaggacga tcgcctcggt cttgaccgtg tttccgggca
     1201 tcctcctact ttgcccggcg ccgcggacgg cggcgcaggc ggacctgaca ggtcgggcag
     1261 aagtaggtgg agcggccgga gacgacgatg cggacgatcg cgccgccgca ggagaggcag
     1321 ggctcgccct cgcgggtgtg gacgaggaac ttctcctgca tcgtgccctt ctcgccgcgg
     1381 ccgtcgcggt agtcgtcgat cgaggagccg ccggcctcga tcccggcctc cagcgaggcg
     1441 acgaccgcgt cgcgcagcgc cagcaggtgc tcgggtttca tcgaaccggc gggcgagagc
     1501 gggtgcaggc gggcgcggta gagcgcctcg tcggcgtaga tgttgccgac cccggcgacc
     1561 cccttctggt cgagcaggaa ggacttcagc ggcaccacgc ggccggccgc catctcgccg
     1621 aacgcctcgg gggtgaactc gggcgagagc ggctcgaccc cgactttgcc ggcgaagcgc
     1681 gtctccaggc cgtcgttggg aagcaggaag gcctcgccga agcggcgcgg gtcggtgaac
     1741 cagacctcgc ggccgtcgtc gagcaggaag cgggcgcgca ggtgacgggc cgaggtcgag
     1801 cgctcgccct cgtagaggcg gcggccctcg gaggggtcga gcacgtcctc gccctcgacc
     1861 aggacgaggt tgccggtcat ccgcaggtgc atgaccaggg tgcgctcgcc gtcgaggccg
     1921 agcagcaggt acttgccccg ccgtcccagc gactcgatcg tgctgccgga gaccgcggcg
     1981 cccagcttct cgggcggcac cggccgcgac cagcgcgcgt cgagcacctc cagctcctcg
     2041 atccggtgcc cttccagctc cggctccagc tgccgcgcaa cggtctcgac ctcgggtagc
     2101 tcgggcacag cccgatttct atcggctact cggcgttggc gcgcgggtgg gcgtcgaagt
     2161 agacctgctt gaggcggtcg cccgagaggt gggtgt
//
LOCUS       DASRIG010000379         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2003676, whole genome shotgun sequence.
ACCESSION   DASRIG010000379 DASRIG010000000
VERSION     DASRIG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003676"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VFB52_10160"
     CDS             complement(<1..1288)
                     /locus_tag="VFB52_10160"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO06740.1"
                     /translation="MESASAERRAAEVEIAKPIGAELRAATDEQIEAAVAASDPMVLR
                     GLLYQLTGDEEVAATATDPVEFLFLEMQFVIDPDQLAVLHRKAIELLKSLRDGDADTV
                     PFGPRERLRASLDLTSGVELADCEAEMFEASTGVEPFVHGLSWEREPEPERLGSFSVA
                     VIGAGMGGLNAGVMLKRAGIPFTILEKNPAVAGTWYENRYPGARVDNPSRAYVHAYGA
                     DYDFPGMFTVQAENERYFNWVADEFELREEIEFETEVESIVWHEDDGEWELRASGPGG
                     ERVVRANAVISAVGFLSRPNLPEIEGMEEFEGDSFHTARWPEGLDLAGKRVAVIGSGC
                     TGYQLVQAIAGQPERTYLLQRTPSWVFGVEGYLSDFPPELAWLNRNLPFYTNFTRFRL
                     AWLFGPESLRAAYELDPEFEGEGARSAHNQKLRDDQL"
     gene            complement(1288..2373)
                     /locus_tag="VFB52_10165"
     CDS             complement(1288..2373)
                     /locus_tag="VFB52_10165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO06741.1"
                     /translation="MKLGVCLNPAHAPGTPVTETAAWDVQVVQWAEELGYSEVWFGEH
                     LTVPWEGCPAPDLLIAQALKETSEIRLGPASFNLPLHHPSMAAHRIAYLDHLSGGRLN
                     LGIGASGTLTDWQLFGIDGLEGENREMTWESLDAILRLWNDPEPFESQGAHWTVNKPA
                     PLFDGKLAFHLTPLQDPGPPISISGLSPESPSLKVAGERGYAPLCLAFGGEYLRGQWA
                     TVEAGAAAAGKSADRNGWGIVYDVFVAETDEEAVDLCLDSGVGQYLREFWLPLMADVG
                     LIAMYKGDPEMSDSEVTADYFLRNAAIVGSVDTVVEKVEAAQAETGGFGTLIQGGQDF
                     RGNPEPMRASMELLAKEVLPRVNGGRS"
BASE COUNT          345 a          836 c          860 g          389 t
ORIGIN      
        1 cgagctggtc gtcgcgcagc ttctggttgt gggcactgcg ggcgccctcg ccctcgaact
       61 cggggtcgag ctcgtaggcc gcgcgcaggc tctcggggcc gaacagccag gccagccgga
      121 agcgcgtgaa gttcgtgtag aagggcaggt tgcggttcag ccaggcgagc tccggcggga
      181 agtccgagag atagccttcg accccgaaga cccagctcgg ggtgcgctgc agcaggtagg
      241 tgcgctcggg ctggccggcg atcgcctgga ccagctggta gccggtgcag ccgctgccga
      301 tcacggcgac gcgcttgccg gcgaggtcga ggccctcggg ccagcgggcg gtgtggaacg
      361 agtcgccctc gaactcctcc atgccctcga tctccggcag gttcggccgc gagaggaagc
      421 cgaccgcgct gatcaccgcg ttggcgcgga cgacgcgctc gccgccgggg ccgctggcgc
      481 gcagctccca ctcgccgtcg tcctcgtgcc agacgatcga ttcgacctcg gtctcgaact
      541 cgatctcctc gcgcagctcg aactcgtcgg cgacccagtt gaagtagcgc tcgttctcgg
      601 cctggaccgt gaacatgccg gggaagtcgt agtcggcgcc gtaggcgtgc acgtaggcgc
      661 ggctggggtt gtcgacccgg gccccgggat agcggttctc gtaccaggta ccggcgaccg
      721 cggggttctt ctcgaggatc gtgaacggga tcccggcccg cttcagcatc acgcccgcgt
      781 tcagcccgcc catgcccgcg ccgatcacgg cgacgctgaa gctgccgagc cgctcgggct
      841 cgggctcgcg ctcccagctg aggccgtgca cgaacggctc gaccccggtg ctggcttcga
      901 acatctcggc ttcgcagtcg gccagctcga ccccggaggt gaggtcgagg ctggcgcgca
      961 ggcgctcgcg cgggccgaac ggaacggtgt cggcatcgcc gtcgcgcagc gacttgagca
     1021 actcgatcgc cttgcggtgc aggaccgcga gctggtcggg gtcgatcacg aactgcatct
     1081 cgaggaagag gaactcgacc gggtcggtcg cggtcgcggc gacctcctcg tcgccggtca
     1141 gctggtagag cagcccgcgc aggaccatcg ggtccgaggc cgcgaccgcc gcctcgatct
     1201 gctcgtccgt ggcggcgcgc agctcggcgc cgatcggctt ggcgatctcg acctcggcag
     1261 cgcgccgctc ggcgctggcc gactccatca ggaccgcccc ccgttgaccc gcggcagaac
     1321 ctctttcgcc agcagctcca tcgaggcccg catcggctcg gggttaccgc ggaagtcctg
     1381 gccgccctgg atcagggtgc cgaagccgcc ggtctcggcc tgcgcggctt ccaccttctc
     1441 gaccacggtg tcgaccgagc cgacgatcgc ggcgttgcgc aggaagtagt ccgcggtgac
     1501 ttcggagtcg gacatctccg ggtcgccctt gtacatcgcg atcaggccga cgtcggccat
     1561 cagcggcaac cagaactcgc gcaggtactg gccgacgccg ctgtcgaggc agaggtcgac
     1621 cgcttcctcg tcggtttcgg cgacgaagac gtcgtagacg atcccccagc cgttgcgatc
     1681 ggcgctcttg ccggccgccg cggcgccggc ctccaccgtg gcccactgcc cgcgcaggta
     1741 ctcgccgccg aaggcgaggc agagcggcgc gtagccgcgt tcgccggcga ccttcagcga
     1801 gggcgattcg gggctgaggc cgctgatgct gatcggcggc cccgggtcct gcagcggggt
     1861 gaggtggaag gcgagcttgc cgtcgaacag cggcgccggt ttgttcacgg tccagtgggc
     1921 gccctgggac tcgaacggct ccgggtcgtt ccagaggcgg aggatcgcgt cgagcgactc
     1981 ccaggtcatc tcgcggttct cgccctcgag cccgtcgatc ccgaacagct gccagtcggt
     2041 gagcgtgccg gaggcaccga tcccgaggtt gaggcggccg ccggagaggt ggtcgaggta
     2101 ggcgatccgg tgcgcggcca tcgatgggtg gtgcagcggc aggttgaacg aggccgggcc
     2161 gaggcggatc tcgctggtct ccttcagcgc ctgggcgatc agcagatcgg gggctgggca
     2221 cccctcccag ggcacggtca ggtgttcacc gaaccagacc tcgctgtagc cgagctcctc
     2281 ggcccactgc acgacttgca cgtcccaggc ggcggtctcg gtgaccggcg ttcccggcgc
     2341 atgggccggg ttcaggcaaa ccccaagctt catctctctc cactccccgg ttctactgct
     2401 taatgaatta gatgattcat atcagatggt
//
LOCUS       DASRIG010000380         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_189976, whole genome shotgun sequence.
ACCESSION   DASRIG010000380 DASRIG010000000
VERSION     DASRIG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189976"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..525
                     /locus_tag="VFB52_10170"
     CDS             61..525
                     /locus_tag="VFB52_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO06742.1"
                     /translation="MRRINIATPDFTYDHEDPEGYKAGMLALGERLGAKESGATVFEL
                     APGQAVCPYHYEIGEEEWLLVLEGNPTLRHPEGSEQLEPWDVVVFPRGPEGAHKVGNE
                     GTGRARVLFFSTVTAPTATVYPDSGKVGIYTGDAETELIVKGEPADYYDGET"
     gene            complement(507..1139)
                     /locus_tag="VFB52_10175"
     CDS             complement(507..1139)
                     /locus_tag="VFB52_10175"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06743.1"
                     /translation="MAIASLRADAARNRARIVAAAAEVFAERGLDASTAEIADRAGVG
                     EATIYRRFPTKDDLVAAIVATKMEETLEIGAECLEEADPWRGLERFLYEMTERASRDQ
                     GVSDAAKDRCVASAELAPQRKRMLDLCTELVRRAQRAGLVREDVAGQDLIFLVTAAGS
                     LHLPFPDLREDLWKRYLGLLLDGLRPEGATKLRPGAPPRRAIELPQVSPS"
     gene            1284..2801
                     /locus_tag="VFB52_10180"
     CDS             1284..2801
                     /locus_tag="VFB52_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO06744.1"
                     /translation="MTSPQTVPTDTDGNRWLALYVLCAGMLMIVLDATIVNVALPSIQ
                     DDLGFSQSSLAWVVNAYMIPFGGLLLLAGRLGDLVGQRRIFLIGLTVFTIASLVCAIS
                     VNQEMLVAARFIQGIGGALASAVILGMIVTMFPEPGNQAKAIGVYGFVASAGGSIGLL
                     LGGVLTDAISWHWIFMINLPIGIAVGYLATRLVSSREGLGLSEGADYPGAALGTVSLM
                     LGVFTILQITEWGWVDARTLGGLAIAAVLFALFIHRQATIDNPLMPLRLFHSRNVVGA
                     NSLQALLVAGMFGMFFLGALYLGRVLEYSPLEVGLAFLPTTVVMGALSLGFSEKLIMR
                     FGPRTTLIPGVALVVVALLLFARTPVDGNYLTDLLPPFLLIGIGVGTAFPSIMTLAMS
                     GATPQDSGLASGLVNTSMQVGGAVGLALLATLSTERSDSLISDGVAEASALNSGYHLA
                     YLVGAGLAAIAVLIAIFVLRPTPMPAMHGAPGEEGAPDGDGSSEGTPEPAYSDAS"
     gene            2829..3686
                     /locus_tag="VFB52_10185"
     CDS             2829..3686
                     /locus_tag="VFB52_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06745.1"
                     /translation="MNGTRDAGRAVVIGGGTMGAGIAAALARAGFGTELVEVSEELAT
                     EGIDHALKTITDGVARNRITAGDGEAAKARLHGRVGIAAATPQPRLAIEAVPERFELK
                     VEILGEAERTLEPQLLATNTSGISITDLAEPLSDPSRFLGLHFFNPVPAMALVEVVLG
                     EQTSESTADAATALVAELGKEAVVVGDSPGFASSRLGLVLGLEAIRMVEQGVAGPADI
                     DRAMVLGYRHPVGPLRLTDIIGLDVRLDIARNLQGVYGDRFAPPPLLEQMVAEGKLGK
                     KVGRGFFEW"
     gene            complement(3694..4893)
                     /locus_tag="VFB52_10190"
     CDS             complement(3694..4893)
                     /locus_tag="VFB52_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZO06746.1"
                     /translation="MGESGVALAVLLAVVCLLGAVAGPVAAKERSADASVIGGRAASI
                     AELPWLAFVQSEMPGGGSACTATVVAPRILLTAGHCVSDLESGSIYKPSRFIAITGVA
                     DLRSARRADETSVSQVLLFPRFKASNLRGDAALLVLSEPVAAPALPLAGVADAALYEG
                     GTDGRIAGWGIAEAGSERGSYRLQVAGTSVAGAGLCKREVARFYPFYSTASQLCALGP
                     PEFITTVCQGDSGGPLIATRPDGTPVQIGITSLGRGDCETDVPGVFTRVDAVSEWVAG
                     WIAAVETGAAAPPIFVPSVHLPFLPRYVAKELVGRTLREDLGARFRRGWGKRASCVRV
                     AREKVKCGVSWFQGPNDYWGTVTIYLRFREDIVVWDSRYRIRWVNDHCWFSSGHRRSC
                     PIATRTR"
     gene            5027..>5813
                     /locus_tag="VFB52_10195"
     CDS             5027..>5813
                     /locus_tag="VFB52_10195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO06747.1"
                     /translation="MPMGQVKIPRAATNFAESPEELGRMGRISGILWMAAAVVGLVGT
                     LLPGSEHASLAWAIGLSSAVFFYGFASVIGWIPWERTSLNTLAIGMVVTVPVVGLALF
                     LTGGSISYIEPLLVLSLLYAAFFFPSRWAWPLSIELVVVAGTPLIYDSNAIDTAFLPR
                     YLAFVVGYIAATWVMVGLKRRLVEAELRQRDIANRDPLTGVGNRRDFDAAMHRELAER
                     TGPRGGRRAVDESPLALMILDLDDFKGINDRYGHPVGDAVLRDA"
BASE COUNT          825 a         2097 c         1992 g          899 t
ORIGIN      
        1 cactcggcca aaggtacccg agcggggttt gggcgcaatc cccggtggta gcttcgccgg
       61 atgagacgga tcaacatcgc gacgcccgat ttcacctacg accacgagga cccggagggc
      121 tacaaggccg ggatgctggc gctcggcgag cggctcggcg ccaaggagtc cggcgccacc
      181 gtgttcgagc tggcgcccgg gcaggcggtc tgcccctacc actacgagat cggcgaggag
      241 gagtggttgc tggtgcttga aggcaacccg actctgcgcc accccgaggg ctcagaacag
      301 ctggagccct gggacgtggt cgtcttcccg cgcgggccgg agggcgccca caaggtcggc
      361 aacgagggca ccgggcgcgc ccgcgtcctc ttcttctcca cggtcaccgc gccgaccgcg
      421 accgtctacc ccgacagcgg caaggtcggc atctacacgg gcgacgccga gaccgagctg
      481 atcgtcaaag gcgagccggc cgactactac gacggtgaga cctgaggcag ctcgatcgcg
      541 cgccgcggcg gagcgcccgg gcgcagcttg gtcgcgccct ccggccgcag gccgtcgagc
      601 agcaggccga ggtagcgctt ccagaggtcc tcgcgcaggt cggggaacgg caggtgcagc
      661 gagccggcgg cggtgacgag gaagatcagg tcctgcccgg cgacgtcctc gcggaccagc
      721 ccggcgcgct gggcgcggcg gaccagctcg gtgcagaggt cgagcatgcg cttacgctgc
      781 ggggcgagct cggcggaggc gacgcagcgg tccttggcgg cgtcggagac gccctggtcg
      841 cggctcgccc gctcggtcat ctcatagagg aagcgttcga gcccgcgcca ggggtcggcc
      901 tcttcgaggc attcggcgcc gatctccagc gtctcttcca tctttgtcgc cacgatcgcc
      961 gcgaccaggt cgtccttggt cgggaagcgg cggtagatcg tcgcctcgcc gacgccggcc
     1021 cggtcggcga tctccgcggt cgaggcatcg aggccgcgct cggcgaacac ctcggccgcc
     1081 gcggccacga tccgcgcccg gttacgggcg gcgtcggcac gcagcgaggc gattgccatc
     1141 gccctatcct cggtggccgg catcgcgagg atcgtactcg atccggaggc ttcactccgt
     1201 tttaacggag cgcctactcc gttttttgct agggtgccgc gttcgtccct cgttcaccag
     1261 gtccccccag accggagatt tccatgactt ccccccagac tgttccgacc gataccgacg
     1321 gcaatcgctg gctcgcgctc tacgttctct gcgcggggat gctgatgatc gtcctcgacg
     1381 cgacgatcgt gaacgtcgcc ctgccctcga tccaggacga cctcggcttc tcgcagtcga
     1441 gcctggcctg ggtcgtcaac gcctacatga tcccgttcgg cggcctgctg ctgctcgccg
     1501 gccggctcgg cgacctggtc ggccagcgcc gcatcttcct catcggcctg acggtcttca
     1561 cgatcgcatc gctggtctgc gcgatctcgg tcaaccagga gatgctggtc gccgcccgct
     1621 tcatccaggg gatcggcggc gcgctcgcct cggccgtgat cctcgggatg atcgtgacca
     1681 tgttccccga gccggggaac caggcgaaag cgatcggcgt ctacggcttc gtcgcctcgg
     1741 ccggaggctc gatcgggctg ctgctcggcg gcgtgctcac cgacgcgatc agctggcact
     1801 ggatcttcat gatcaacctg ccgatcggga tcgcggtcgg ctacctggcg acccgcctgg
     1861 tctcgagccg cgaaggcctc ggcctcagcg agggcgccga ctatccgggc gccgcgctcg
     1921 gcacggtctc gctgatgctc ggcgtgttca cgatcctgca gatcaccgag tggggttggg
     1981 tcgacgcccg cacgctcggc ggtctcgcca tcgccgcggt cctgttcgca ctcttcatcc
     2041 accgccaggc gacgatcgac aacccgctga tgccgctgcg cctcttccac tcgcgcaacg
     2101 tggtcggcgc caactcgctg caggcgctgc tggtcgccgg gatgttcggg atgttcttcc
     2161 tcggcgccct ctacctcggc cgcgtgctcg aatactcgcc gctcgaggtc gggctcgcct
     2221 tcctgccgac cacggtcgtg atgggcgcgc tctcgctcgg cttctcggaa aaactgatca
     2281 tgcgcttcgg cccgcgcacc acgctgatcc ccggtgtggc cctggtcgtc gtcgcgctgc
     2341 tgctgttcgc ccgcaccccg gtcgacggca actacctgac cgacctgctg ccgccgttcc
     2401 tgttgatcgg gatcggcgtc ggcaccgcgt tcccgtcgat catgaccctc gcgatgtcgg
     2461 gcgcgacgcc gcaggactcc gggctcgcct cgggactggt caacaccagc atgcaggtgg
     2521 gtggcgcggt tggcctggcg ctcctggcga cgctctcgac cgagcgctcg gactcgctga
     2581 tctccgacgg ggtcgccgaa gcctcggcgc tcaactccgg ctaccacctc gcctatctgg
     2641 tcggcgccgg gctggcggcg atcgcggtcc tgatcgcgat cttcgtcctc cgcccgaccc
     2701 cgatgccggc gatgcacggg gcaccgggcg aggaaggtgc tccggacggc gacgggtcct
     2761 ccgagggcac ccccgaaccg gcgtattccg acgccagctg aggcgcgccg ggggataagc
     2821 tcccagtggt gaatgggacg agagatgccg gccgcgccgt cgtcatcggt ggcggcacga
     2881 tgggcgcggg tatcgccgcc gcgctcgccc gcgccggttt cggcaccgag ctggtcgagg
     2941 tcagcgagga gctggccacg gagggcatcg accacgcgct gaagacgatc accgacggcg
     3001 tggcccgcaa ccggatcacc gccggcgacg gcgaagcggc gaaggctcgc ttgcacggcc
     3061 gagtcgggat cgcggcggcg acgcctcagc cgcggctggc gatcgaggcg gtgcccgagc
     3121 gcttcgagct gaaggtcgag atcctcggcg aggccgagcg cacgctcgag cctcaactac
     3181 tcgcgaccaa caccagcggc atctcgatca ccgacctcgc cgagccgctc agcgacccct
     3241 cgcgcttcct cggcctgcac ttcttcaacc cggtgccggc gatggcgctg gtcgaggtgg
     3301 tgctcggcga gcagacctcg gagagcaccg ccgacgccgc caccgccctg gtcgccgagc
     3361 tcggcaagga ggcggtcgtg gtcggcgact cacccggctt cgccagcagc cgcctcggcc
     3421 tcgtgctcgg cctcgaggcg atccggatgg tcgaacaggg agtcgccggt cccgccgaca
     3481 tcgaccgcgc gatggtgctc ggctaccgcc acccggtcgg cccgctgcgc ctcaccgaca
     3541 tcatcggcct cgacgtccgc ctcgacatcg cccgcaacct gcagggcgtc tacggcgacc
     3601 gcttcgcccc gccgcccctg ctcgagcaga tggttgccga aggcaagctc ggcaagaagg
     3661 tcggtcgcgg cttcttcgag tggtgagccg gcgctaacgc gtccgcgtcg cgatcgggca
     3721 gctgcggcgg tggccgctgc tgaaccagca gtggtcgttg acccagcgga tccggtagcg
     3781 ggagtcccag acgacgatgt cctcgcggaa acgcaggtag atggtgaccg tgccccagta
     3841 gtcgttcggg ccctggaacc aggagacgcc gcacttgacc ttctcccgcg cgacccggac
     3901 gcagctggcc cgcttgcccc agccgcggcg gaatcgggcc ccgaggtctt cgcgcagcgt
     3961 gcgcccgacc agttccttgg cgacgtagcg ggggaggaac gggagatgga cgctggggac
     4021 gaagatcggc ggcgcggccg cgccggtttc aaccgcggcg atccagccgg cgacccattc
     4081 cgagaccgcg tcgacgcggg tgaagacccc gggcacgtcg gtctcgcagt cgccgcggcc
     4141 gaggctggtg atcccgatct ggacgggcgt gccgtcgggc cgggtggcga tcagcggccc
     4201 gccgctgtcc ccctggcaga cggtggtgat gaactcgggt ggcccgagcg cgcagagctg
     4261 cgacgcggtc gagtagaagg ggtagaaacg cgcgacctcg cgcttgcaga gtccggctcc
     4321 ggcgacgctg gtgcccgcga cctggagccg gtagctgccg cgttcggaac cggcctcggc
     4381 gatcccccag ccggcgatcc ggccgtcggt cccgccttcg tagagggcgg cgtcggcgac
     4441 gcccgccagc ggtagggccg gcgccgcgac cggctcgctc agcacgagca gggcggcatc
     4501 cccgcgcagg ttcgatgcct tgaagcgagg gaagagcagc acctgtgaga ccgaggtctc
     4561 gtcggcgcgt cgagccgacc gcaggtcggc gaccccggtg atcgcgatga agcgcgaggg
     4621 tttgtagatc gagccggatt cgaggtcgct gacgcagtgg ccggcggtca gcaggatccg
     4681 cggtgcgacc acggtggccg tgcaggcaga accgccgccc ggcatctcgg actggacgaa
     4741 ggcgagccac ggcagctcgg cgatcgaggc ggcgcggcca ccgatcaccg aggcatcggc
     4801 cgagcgctcc ttcgccgcca ccggaccagc cacggcgccc agcaggcaga ccacggcaag
     4861 cagcaccgcg agcgcgacgc cggactcccc cacgactgtg agcctccccc tgattcaaac
     4921 gatgctagcc gaccggtctg ggctcttcgc acacttgtgg ttcgcaccac gcggcgcggc
     4981 aatcggcact aaagcccccc gcccgtgcca ccgatgggac ctagagatgc cgatgggcca
     5041 ggtcaagatt ccaagagcag cgaccaactt tgccgaatcc cccgaggagc tcggtcgcat
     5101 gggccggatc tccgggatcc tctggatggc cgccgccgtg gtcggcttgg tcggcacgct
     5161 gctgccggga tcagagcacg ccagcctcgc ctgggcgatc gggctcagct cggcggtctt
     5221 cttctacggc ttcgccagtg tgatcggctg gatcccctgg gaacggacct cgctgaacac
     5281 gctggcgatc gggatggtcg tcacggtccc cgtcgtcggg ctcgcgctct tcctgaccgg
     5341 cggctcgatc agctacatcg agccgctgct ggtcctctcc ctgctctacg ccgccttctt
     5401 cttcccctcg cgctgggcct ggccgctttc gatcgagctg gtcgtggtcg ccggcacgcc
     5461 gctgatctac gactcgaacg cgatcgacac cgccttcctc ccccgctacc tcgccttcgt
     5521 cgtcggctac atcgccgcga cctgggtgat ggtcgggctc aaacggcgcc tggtcgaagc
     5581 cgagctgcgc cagcgcgaca tcgccaaccg cgacccgctc accggggtcg gcaaccgccg
     5641 cgacttcgac gccgccatgc accgcgagct cgccgaacgc accggtccgc gtggcggccg
     5701 ccgcgccgtc gacgagagcc cgttggcgct gatgatcctc gacctcgacg acttcaaggg
     5761 gatcaacgac cgctacggcc acccggtcgg cgacgcggtt ctccgcgacg ccg
//
LOCUS       DASRIG010000381         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_190470, whole genome shotgun sequence.
ACCESSION   DASRIG010000381 DASRIG010000000
VERSION     DASRIG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190470"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFB52_10200"
     CDS             complement(<1..714)
                     /locus_tag="VFB52_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZO06748.1"
                     /translation="MGTPPYVELHAHSAFSFLDGASTPLELAAAAEELGYAALALTDH
                     DGIWGSREFAEACTELELRAITGAELTVALPTEGDAAGPDLRHLTLLAEDQRGYRNLC
                     RLLTAAHAHTRDNTARTATQPWATLEQVEEHAEGLVCLSGCARDGALAGCWERGDTAL
                     GERLGRRLLAAFGRERFRVELQLPLWRHDRARNRWLTLLAERLGVATVATGNVHSHGS
                     RRARLQDALVAVGRSETLEE"
     gene            complement(723..974)
                     /locus_tag="VFB52_10205"
     CDS             complement(723..974)
                     /locus_tag="VFB52_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06749.1"
                     /translation="MSTVLRRLYSPEPVEVRTAADGVPTAVAGVAVEALRERWVVDDR
                     WWVPRAWLHREYFELALADGCAVVVFRSLHSEHWFKQRA"
     gene            complement(971..1954)
                     /locus_tag="VFB52_10210"
     CDS             complement(971..1954)
                     /locus_tag="VFB52_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06750.1"
                     /translation="MAERTEATARALEAIGAAVESERPGEVFFAIDGLRGIHGGDRAG
                     VLAAARAAVRPVFGKDGEARLGVAPTRTAAFAAAHRRDGSVVHPAGLPGFLAPLRVTI
                     LRHRLDAPAPERETLVVALERLGLERLGKLARLHSDQVADRFGQLGLAALRIARGEDE
                     PLRPRTPHEELVEQLELPEGTAGSQLDRALELLVDRLLAAPQRRGRTLLGLRLGALLA
                     GGGSWSVEQGLGRPSAAAPVLRRVLGPRLESLPGPATALRLRALGFGPAAADQLELAV
                     GGEGPRRRRVGAAVREVRATQGPESLLKILPVATASRIPERWALLTPFPEL"
BASE COUNT          317 a          859 c          871 g          272 t
ORIGIN      
        1 ctcctccagg gtctcgctgc ggccgaccgc gacgagcgcg tcctgcaggc gggcacggcg
       61 agagccgtgc gagtgcacgt tgccggtcgc cacggtggcg acgccgagcc gctcggcgag
      121 cagggtgagc cagcggttgc gggcgcggtc gtggcgccag agcggcagct gcagctcgac
      181 ccggaagcgc tcgcggccga aggcggcgag caggcggcgg ccgagccgct cgccgagcgc
      241 cgtgtctccg cgctcccaac agccggcgag ggcgccgtca cgggcgcagc cggagaggca
      301 gaccagcccc tcggcgtgct cctccacctg ctccagggtc gcccacggct gggtcgcggt
      361 gcgggcggtg ttgtcacggg tgtgggcgtg cgccgcggtc agcagccggc agaggttgcg
      421 gtagccgcgc tggtcttcgg ccagcagggt gaggtggcgc aggtcgggtc ccgcagcgtc
      481 gccctcagtg ggcagcgcca cggtcagctc ggcgccggtg atcgcccgta gctccagctc
      541 ggtgcaggcc tcggcgaact cgcgtgagcc ccagatcccg tcgtggtcgg tcagcgccag
      601 cgccgcgtag ccgagctcct cggcggcagc ggccagctcc agcggggtcg aggcgccgtc
      661 gaggaaggag aaggccgagt gcgcgtgcag ctctacgtaa ggaggggtgc ccacgactct
      721 cctcaggccc gctgcttgaa ccagtgctcg ctgtgcagcg agcggaagac gacgaccgcg
      781 caaccgtcgg cgagcgccag ctcgaagtac tcgcggtgca gccaggcgcg cgggacccac
      841 cagcggtcgt cgaccaccca gcgctcgcgc agggcctcga ccgcgacccc ggcgaccgcg
      901 gtcgggacgc cgtcggcagc ggttcggacc tcgaccggct cgggcgagta gagacggcgc
      961 aggaccgtgc tcatagttcc gggaagggag tgagcagcgc ccagcgctcg gggatccgcg
     1021 aagcggtcgc gaccgggagg atcttcagca acgactccgg cccctgggtg gcgcgcacct
     1081 cgcgcaccgc cgcgccgacc cggcggcggc gcgggccctc gccgccgacc gcgagctcga
     1141 gctgatcggc ggcggccggg ccgaagccga gcgcccgcag ccgcagcgcc gtcgccggcc
     1201 ccggcaacga ctccagccgc gggccgagca cgcggcgcag gaccggggcc gcggcgctgg
     1261 ggcgacccag cccctgctcg acgctccagc tgccgccgcc ggcgagcagc gccccgaggc
     1321 gcaggccgag cagggtgcgg ccacggcgct gcggcgctgc cagcaggcgg tcgaccagca
     1381 gctcgagcgc gcggtcgagc tgactgcccg ccgtcccctc cggcagctcc agctgctcga
     1441 ccagctcctc gtgcggggtg cgcgggcgca gcggctcgtc ctcgccgcgg gcgatccgca
     1501 gcgccgccag gccgagctgg ccgaagcggt cggcgacctg gtcggagtgc agccgcgcca
     1561 gcttgccgag gcgctcgagc ccgagccgtt cgagcgcaac gacgagcgtt tcgcgctcgg
     1621 gcgcgggagc gtcgaggcgg tggcgcagga tcgtcacccg cagcggcgcc aggaacccgg
     1681 gcaggccggc cgggtggacg acgctgccgt cgcgccggtg ggcggcggcg aaggcggcgg
     1741 tacgagtagg cgctaccccg aggcgcgcct ccccgtcctt gccgaacacg gggcgcaccg
     1801 ccgccctcgc cgccgccagc accccggccc ggtcgccgcc gtggatcccg cgcagcccgt
     1861 cgatcgcgaa gaagacctcg cccggccgct cgctctcgac cgccgccccg atcgcctcca
     1921 gcgcccgcgc cgtcgcctcc gtccgctccg ccaccgcgac cggatccgca agcggtgcgc
     1981 gcccggatcg ggaccccccg gaacgatccg ggcgcgcggg agtcgccagg cgttcatcga
     2041 tgacgcaagc gatctccata cgaacgtatg ttcgtatatc gagagcctcg attcaagacc
     2101 caatttgagc caatttttgg ctctacagag ccaacttgtg cttaaccaca agttgctggg
     2161 aagacttccg ggagcgaccc cgaacgggcg gcgcggctag tccgcttggg cgatcttcgc
     2221 tgcctcggcg gtgccgaggt aaggcgtcag tgtgaactca accaggtcag ggaggagctg
     2281 ctccagctcc gccgtctgcc cggcgatgat gcggcggga
//
LOCUS       DASRIG010000382         7737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_191199, whole genome shotgun sequence.
ACCESSION   DASRIG010000382 DASRIG010000000
VERSION     DASRIG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191199"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="murF"
                     /locus_tag="VFB52_10215"
     CDS             complement(<1..1094)
                     /gene="murF"
                     /locus_tag="VFB52_10215"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZO06751.1"
                     /translation="MNLSPDQIAAALGAEVLAEGGPGSPARATIDSSQTGPGDLFFGL
                     RGANRDGGEFAAAALEAGAWGAVVGPAFRGSFPDTESAKEPHGGEGGWVFAVEDPLAA
                     MQALAREYRRVLGARVIGVTGSVGKTSVKDITRSLIPGRVHANKQNLNTEIGLPLTLL
                     EAPEDTETLVLEMAMRGAGQIRELGEIAEPDVAVITNVGPVHVELLGSVEAIAAAKAE
                     ILATLRPGGAAVAPVEAGELEPHLAGVERLLRFGPGGEVEAREARVVDGLTEALVATP
                     LGEQRFHFPFAEAHNLTNALAAIAAGAALGIPPGDMADGAASIGFSRFRGERLELRDG
                     IVLVNDCYNANPVSMRAALDHLATLGAPRTL"
     gene            complement(1091..2593)
                     /locus_tag="VFB52_10220"
     CDS             complement(1091..2593)
                     /locus_tag="VFB52_10220"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZO06752.1"
                     /translation="MLLSDLIAGLDPVSVQGEPGVEITALAYDSRRVEPGTLFFCVRG
                     ERADGHRFGPGAVRDGAAALVVEEVLDVEVPQVVVADARAAMAPLAARFWGDPTAELR
                     VVGVTGTNGKTTTAFLAREILEAAGVQCGLLGTVKQVVGGVEEEVERTTPEAIELQAT
                     FRRMLQGGDRACVMEVSSHALALHRADAIHFEVAVFTNLTQDHLDFHADMEDYFQAKR
                     LLFAARPKAAIVNVDDPYGARLAGEFPACISFSAAGAEADYSAREVSFDVGGASFTLH
                     AESQALKRSTRELDFPIGHDIPVRTQLPGDFNVANALGAIAAAARLDVDPRAAAEALS
                     AAARVPGRFEPIDEGQDFAVLVDYAHTPDSMENVLRAARRLTAGRLISVFGAGGDRDR
                     DKRPLMGRAGGELSDLAIVTSDNPRSEEPAAIVGEVAAGARGQAAEVRTEVDRRAAIA
                     LALAEAQGGDTVVIAGKGHEQGQEFEGGRKVPFDDRDVAREELRRLGSQA"
     gene            complement(2624..4276)
                     /locus_tag="VFB52_10225"
     CDS             complement(2624..4276)
                     /locus_tag="VFB52_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZO06753.1"
                     /translation="MRLVERRIGLLFAAFLLCFLVVVARAFWLQGVQGSSLASQAASQ
                     QSETVLVPGMRGSLLDRRGNELATSEDAATIYATPYQVKNPPVAAEKLAKILDLEPAE
                     VLGDLTEESGFSYIAKKVSLEQAGRIERLHLEGIGSLPDSRRTYPQGEMAGQVIGSIG
                     SENEGLTGLEMGEEEVLGGSDGERRIVTDALGDPIRLETVKSTEDGEDIQLTLDPLIQ
                     EKTEAALAEIGETYTPNGATAIVVDPRSSEILAMANWPPVNPGDLSEVSNADLVNRAT
                     GFTFEPGSTFKAFTVAAALEDKDVTPTSTFTLEPTIQVADRTIEESHERGTETMDVGQ
                     ILAQSSNVGAVTIGLEVGADRFSKWINRFGFGRQTGVQFPGEEIGLVPELDEYSGSTM
                     GNLPIGQGLSVTPMQMVAGYTAIANGGMLKRPQLIERIGEEKIHEPKGKRVVSPEVAA
                     EVREMLEGVLAPGGTASEVSVPGYTLAGKTGTAQVVEDGEYSETHYIASFIGFAPADD
                     PQLLAAVIVDEPQGEIYGGSVAAPAFGQIASFALPYLGVAPE"
     gene            complement(4288..4905)
                     /locus_tag="VFB52_10230"
     CDS             complement(4288..4905)
                     /locus_tag="VFB52_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06754.1"
                     /translation="MAVAVPKRRTAPAPPPSRTTPPKRKAAPKRKSAAAPRGRAVAAP
                     APSRAPARQPARKPARKAPARTPQRVRPKVRRKPGARVIPLAGRTAVAVTHLPDSGLI
                     VRLTRGRAWIAVLGVLLVGIVGLNVINLSFSAAAAKIDEQIITLEGEIQMLKTRDATR
                     SSTKRVRGAAREFGFATAAGSESVFVEYGPDTIETAAARLAAASP"
     gene            complement(4905..5942)
                     /gene="rsmH"
                     /locus_tag="VFB52_10235"
     CDS             complement(4905..5942)
                     /gene="rsmH"
                     /locus_tag="VFB52_10235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZO06755.1"
                     /translation="MRLHAARTHNLPPTGRYRDHSSANRPIDLATMSTHEPVLAAELV
                     AFLAPQPGQTAVDCTFGGGGHARLVAEQLGAEGTLIGIDRDPAAAQRFAAFSAEAPCR
                     TLFIPANFAEGLEALRGEGIRPDMIYMDLGMSSMQVDAWERGFSYAYDAPLDMRMDTR
                     QQFDAADLVNEWPEARIAQVLRRFGEERYAGGIAREIVRRRPLRTTSDLVEAIKAGMP
                     ASARFGAGHPAKRSFQAIRIAVNGELDALNEALPTAWELLPVGGRFAAIAFHSREDRP
                     VKQFLAAKARGCVCPPELPVCVCGHEPEAELLTRRAVSPSPEEVEANPRSRSAHLRVA
                     QKIALPTGDAG"
     gene            complement(5945..6385)
                     /gene="mraZ"
                     /locus_tag="VFB52_10240"
     CDS             complement(5945..6385)
                     /gene="mraZ"
                     /locus_tag="VFB52_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZO06756.1"
                     /translation="MAFRGLYEHSLDSKDRLTVPSRFRASLADGIVLSKGFDPCVWVH
                     TTDEFEQLSDRFLSPHSPFGRDARQLRRRFHGGSFDEKLDSAGRIRIPKPLLEHAGLK
                     GNCVVIGAGEYLEIWDADAWAKQELELDAAAPEIAEGLASAGTA"
     gene            6582..7475
                     /locus_tag="VFB52_10245"
     CDS             6582..7475
                     /locus_tag="VFB52_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06757.1"
                     /translation="MSTPEGAPPPPQAPPEPLGETVDDANDLRGAHFKALLSRPLTWI
                     LVIGCAIAGGVAGAIFLNAPLGGAILAGLVLVGIGIVFAIADSRAADAFFAIYAQERG
                     LTLVSDRGRLPEATPLLRKGDDRYTQRSLLGPLGPGCEGTLALYTYEEEYRDSKGNKQ
                     TNYYHYTVGFIEIEEVRALVPELYCQRKFGFRALEKFEDVFRGSKERVNLESEAMVDK
                     YELFANENQDAVWLRRLFSPTFIVWLTDSAPEKFAFELVNGRLCCYVSGHKEKADQLD
                     TVMAASVAVATRLREEATQTS"
     gene            complement(7528..>7737)
                     /locus_tag="VFB52_10250"
     CDS             complement(7528..>7737)
                     /locus_tag="VFB52_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZO06758.1"
                     /translation="SRRFGSPLADQLRRQATALLRDSRRAVEERAARAAPKIQLVVAL
                     VLVPSVLLMIAAGLIANAGTLLAGF"
BASE COUNT         1109 a         2732 c         2743 g         1153 t
ORIGIN      
        1 agcgtgcgcg gcgccccgag ggtcgcgagg tggtcgagcg ccgcgcgcat cgacaccggg
       61 ttggcgttgt aacagtcgtt cacgaggacg attccatcgc gaagctccaa ccgctctccg
      121 cgaaaccggg agaagcctat gctcgcagcg ccgtcggcca tatccccggg tgggatgccg
      181 agcgcggcgc cggcggcgat cgcggccagg gcgttggtca ggttgtgggc ctcggcgaag
      241 ggaaagtgga agcgctgctc gcccagcggg gtggcgacga gcgcctcggt gaggccgtcg
      301 acgacgcgcg cctcgcgggc ctcgacctca ccgccggggc cgaagcggag caggcgctcg
      361 acgcccgcca ggtgcggctc cagctcgccc gcctcgaccg gcgcaaccgc ggcgccaccc
      421 gggcgcagcg tcgccaggat ctccgccttg gccgccgcga tcgcctccac cgagccgagc
      481 agctcgacgt ggaccgggcc gacgttggtg atcacggcga cgtcgggctc ggcgatctcg
      541 cccagctcgc ggatctgccc ggccccccgc atcgccatct ccaggaccag cgtctcggtg
      601 tcctcggggg cttcgagcag ggtcagcggc agcccgatct cggtgttcag gttctgcttg
      661 ttggcgtgca cccgccccgg gatcagcgag cgggtgatgt ccttgaccga ggtcttgccg
      721 accgagccag tgaccccgat cacgcgcgcg cccagcaccc gccggtactc gcgcgccaac
      781 gcctgcatcg cggcaagcgg atcctccacc gcgaagaccc agcccccctc cccaccgtgc
      841 ggttccttag cggactcagt gtccggaaag gaaccgcgga acgcggggcc gacgacagcc
      901 ccccaggcgc cggcctcgag cgccgcggcg gcgaactcgc cgccgtcgcg gttggcgccg
      961 cgcaggccga agaagaggtc gccggggccg gtctgggagg agtcgatcgt cgcccgggcc
     1021 ggcgagccgg gcccgccctc ggcgagcacc tcggcgccga gcgcggcggc gatctggtcg
     1081 gggctcaggt tcacgcctga ctacccaacc gccgcagctc ctcgcgggcg acgtcgcggt
     1141 cgtcgaaggg gaccttgcgg ccgccctcga actcctggcc ctgctcgtgg cccttgccgg
     1201 cgatcaccac ggtgtcgccg ccctgcgcct cggccagggc cagggcgatc gcggcccgcc
     1261 ggtcgacctc ggtccgcacc tcggccgcct gcccccgcgc cccggccgcg acttccccga
     1321 cgatcgccgc cggctcctcg gagcgcgggt tgtcggaggt gacgatcgcc aggtccgaga
     1381 gctccccccc ggcccggccc atcagcgggc gcttgtcgcg atcgcgatcg ccgcccgccc
     1441 cgaagacgga gatcagcctg cccgcggtca gccgacgcgc cgcccgcagc acgttctcca
     1501 tcgagtccgg cgtgtgcgcg tagtcgacca gcacggcgaa atcctggccc tcgtcgatcg
     1561 gctcgaaccg gccgggcacc cgggcggccg ccgagagcgc ctcggccgcg gcgcgcggat
     1621 cgacgtccag cctcgccgcc gccgcaatcg ctccgagcgc gttggcgacg ttgaagtcgc
     1681 cgggcagctg cgtgcgcacg gggatgtcgt gaccgatcgg gaaatcgagc tcgcgcgtgc
     1741 tccgcttcag cgcttgcgat tcggcatgaa gggtgaagga cgcgccgccg acgtcaaagg
     1801 agacctcgcg ggccgagtag tcggcctccg cccccgcggc ggagaaggag atgcaggcgg
     1861 ggaattcacc cgcgagccgc gctccgtagg gatcgtcgac gttgacgatc gccgccttcg
     1921 ggcgagcggc aaagagcagg cgcttggcct ggaagtagtc ctccatgtcc gcgtggaagt
     1981 cgaggtggtc ctgggtcagg ttggtgaaca cggcgacctc gaaatggatc gcgtcggcgc
     2041 ggtgcagggc gagcgcatgc gaggagacct ccatcacgca agcccggtct cccccttgaa
     2101 gcatgcgccg aaacgtcgcc tgcagctcga tcgcctccgg ggtcgtgcgt tcgacctcct
     2161 cctccacccc gccgaccacc tgcttgaccg tgcccagcag cccgcactgg acgcccgccg
     2221 cctcgaggat ctcccgggcc aggaacgcgg tcgtcgtctt cccgttcgtg ccggtgacgc
     2281 cgaccacacg cagctcggcg gtcgggtcgc cccagaagcg cgccgccagc ggcgccatcg
     2341 ccgcccgcgc gtcggcaacc accacctgcg gcacctcgac gtcgagcact tcctcgacga
     2401 ccagcgccgc cgctccgtcg cgcaccgccc ccggcccgaa ccggtgcccg tcggcccgct
     2461 cgccgcgcac gcagaagaac aacgtccccg gctcgaccct gcggctgtcg taggcgaggg
     2521 cggtgatctc gacgcccggc tcgccctgca cggaaaccgg gtcgagcccg gcgatcaggt
     2581 cgctgagcag catcggctca ggatatggag cggcgcggtt ggcctattcg ggcgcgacgc
     2641 ccaggtaggg cagggcgaag gaggcgatct gcccgaaggc cggggcggca accgagccgc
     2701 cgtagatttc gccctgcggt tcgtcgacga tcaccgcggc cagcagctgc gggtcgtctg
     2761 ccggggcgaa gccgatgaag gaggcgatgt agtgggtctc cgagtattcg ccgtcttcga
     2821 cgacctgggc ggtgccggtc ttgccggcga gcgtgtagcc ggggacgctg acctcggagg
     2881 cggtaccgcc cggcgccagc acgccctcga gcatttcgcg cacctcggcg gcgacctcgg
     2941 ggctgaccac gcgcttgccc ttcggctcgt ggatcttttc ttcgccgatc cgctcgatca
     3001 gctgcggccg tttcagcatc ccgccgttgg cgatcgcggt gtagccggcg accatctgca
     3061 tcggcgtcac cgagagaccc tgcccgatcg gcaggttgcc catcgtcgag ccggagtatt
     3121 cgtcgagctc cgggaccagg ccgatctcct caccggggaa ctggaccccg gtctggcggc
     3181 cgaagccgaa gcggttgatc catttgctga agcggtcggc gccgacctcg agcccgatcg
     3241 tgaccgcgcc cacgttcgag gactgggcga ggatctggcc gacgtccatc gtttcagtac
     3301 cgcgttcgtg cgactcctcg atcgtgcggt cggcgacctg gatcgtcggt tccagggtga
     3361 aggtgctggt cggggtcacg tctttgtcct ccagcgccgc cgccacggtg aacgccttga
     3421 aggtcgagcc cggctcgaag gtgaagcccg tcgcccggtt gaccaggtcc gcgttgctca
     3481 cttccgagag atcgccgggg ttgaccggcg gccagttcgc catcgccagg atctccgagc
     3541 tgcgcgggtc gacgacgatc gccgtcgccc cgttcggcgt gtacgtttcg ccgatttcgg
     3601 ccagcgccgc ttccgtcttc tcctggatca gcgggtcgag cgtgagctgg atgtcttcgc
     3661 cgtcctcggt gctcttcacc gtttccagcc ggatcgggtc gccgagcgcg tcggtgacga
     3721 tgcgccgttc gccgtcgctg ccgccgagca cttcttcctc gcccatctcg aggccggtca
     3781 gcccctcatt ttcggagccg atcgagccga tcacctggcc cgccatctcg ccctgcgggt
     3841 aggtgcggcg gctgtcgggc agcgagccga tgccttcgag gtggagccgc tcgatccgcc
     3901 ccgcctgttc gagcgaaact tttttggcga tgtaggagaa gccgctttcc tcggtcaggt
     3961 cgccgagcac ctcggccggc tccaggtcga ggatcttggc cagcttctcc gccgcgaccg
     4021 gcgggttttt cacctggtag ggggtcgcgt agatcgtcgc cgcgtcctcg gaggtggcga
     4081 gctcgttgcc gcggcggtcg agcaggctgc cgcgcatgcc cggcaccagc acggtttcgg
     4141 actgctggga ggccgcctgg gaggcgagcg aagagccctg cacgccctgc aaccagaagg
     4201 cgcgggcgac gaccaccaag aagcagagca ggaaggcggc gaagagcagc ccgatcctgc
     4261 gttcgaccag tcgcaccgcg gcagccccta gggcgaggcc gctgccaggc gggcggcagc
     4321 ggtctcgatc gtgtcggggc cgtattcgac gaagacggat tccgagccgg cggcggttgc
     4381 gaagccgaac tcacgggcgg cgccacgcac ccgtttggtg ctcgagcggg tcgcgtcgcg
     4441 ggttttcagc atctggattt cgccttccag ggtgatgatc tgctcgtcga tcttggccgc
     4501 ggcggcactg aagctgaggt tgatcacgtt gaggccgacg atcccgacca ggagcacgcc
     4561 gagcacggcg atccaggcgc ggccgcgggt cagccggacg atcaggcccg agtcgggcag
     4621 gtgggtgacc gcgaccgcgg tgcggccggc aagcgggatg acgcgggcgc cgggtttgcg
     4681 gcggaccttc gggcggaccc gctgcggagt gcgcgcgggg gctttgcgag cgggtttgcg
     4741 ggccggctgg cgcgccgggg cgcggctcgg ggccggcgcg gcgaccgcgc gtccccgcgg
     4801 tgcggcagcg cttttgcgtt tcggcgcggc tttgcgtttc ggcggcgtcg tccgcgaggg
     4861 cggcggcgcc ggcgcggtgc gtcgtttggg gacggcgacg gccatcagcc cgcatccccc
     4921 gtcggcagcg cgatcttctg cgccacccgc aggtgggcgg agcgcgagcg cgggttcgct
     4981 tcgacctcct cggggctggg cgagacggcg cggcgggtca gcagctccgc ctcgggctcg
     5041 tgaccgcaga cgcagaccgg cagctccggc gggcagacgc agccgcgcgc tttggcggcg
     5101 aggaactgct tcaccggccg gtcctcacgc gagtggaaag cgatcgcggc gaagcggccg
     5161 ccgactggga gcagctccca ggccgtcggc agcgcctcgt tcagcgcgtc gagctcgccg
     5221 ttgacggcga tccggatcgc ctggaagctg cgcttggctg ggtggcccgc gccgaagcgg
     5281 gccgaggcgg gcatccccgc cttgatcgct tcgaccaggt ccgaagtcgt gcgaagcggc
     5341 cgccggcgga cgatctcgcg ggcgatcccg ccggcgtagc gctcctcgcc gaaacggcgc
     5401 agcacctgcg cgatccgcgc ctccggccac tcgttgacga ggtcggcggc gtcgaactgc
     5461 tggcgcgtgt ccatgcgcat gtcgagcggg gcgtcgtagg cgtaggaaaa gccgcgctcc
     5521 caggcatcga cctgcatcga ggacattccg aggtccatgt agatcatgtc tgggcgtatg
     5581 ccttcgccac ggagggcctc gagcccttct gcgaagtttg cagggataaa gagagtccgg
     5641 cagggcgctt cggccgagaa ggcggcaaaa cgctgggcgg ccgcggggtc gcgatcgatc
     5701 ccgatcagcg tcccctcggc gccgagctgc tcggcgacca gccgggcgtg gccgccgccg
     5761 ccgaaggtgc agtcgaccgc cgtctgaccc ggctgcgggg ccaggaaggc gaccagctcg
     5821 gcggcgagaa cgggctcgtg cgtgctcatc gtcgccaggt cgatcggccg gttggccgac
     5881 gagtggtcgc ggtagcggcc ggtcggtggg aggttgtggg tgcgggcggc atgcagtctc
     5941 atgatcacgc ggtcccggcg ctggcgagac cctcggcgat ctccggcgcg gcggcgtcga
     6001 gttccagctc ctgtttggcc caggcgtcgg cgtcccagat ctccaggtac tcccccgccc
     6061 cgatcaccac gcagttgcct ttcaatccgg cgtgctccag aagtggcttg ggaatgcgga
     6121 tgcggccggc cgagtccagc ttctcgtcga aggagccacc gtggaagcgg cggcgcagct
     6181 gcctggcgtc gcgcccgaag gggctgtgcg gcgagaggaa gcggtcggag agctgctcga
     6241 actcgtcggt cgtgtgcacc cagacgcagg ggtcgaagcc cttggagaga acaattccgt
     6301 cagcgaggga cgcgcgaaag cgcgaaggaa ccgtgagccg gtccttggaa tcgaggctgt
     6361 gctcgtaaag gccccggaat gccacctcgg aaagtccttt ctgagcggag aaggccggaa
     6421 aaaggagttg ggaccggagt gatgaggtgg gaggagcctc accaccccgg tcctgggctt
     6481 caactcgatc gccacaataa cccacactcc cccactttgc aacaccacct ccctctgcaa
     6541 ccccatttgc atccgctggc gcgcgcggta ggttgctccg cgtgagcacg cccgagggcg
     6601 ccccgccgcc acctcaagcc cctcccgagc cgctcgggga gaccgtcgac gacgccaacg
     6661 acctgcgcgg cgctcacttc aaggcgctgc tcagccggcc gctgacctgg atcctggtga
     6721 tcggctgcgc gatcgccggc ggcgtcgccg gcgcgatctt cctgaacgcc ccgctcggcg
     6781 gcgcgatcct cgccggcctc gtcctggtcg gaatcgggat cgtcttcgcg atcgccgact
     6841 cacgcgccgc cgacgccttc ttcgcgatct acgcgcagga acgcggcctc accctggtca
     6901 gcgaccgcgg ccgtctgccg gaagcgaccc cgctgctgcg caaaggcgac gaccgctaca
     6961 cccagcgcag cctgctcggc ccactcggcc cgggctgcga aggcaccctg gcgctctaca
     7021 cctatgagga ggagtaccgg gacagcaagg gcaacaagca gaccaactac taccactaca
     7081 cggtcggctt catcgagatc gaagaggtcc gcgccctcgt ccccgagctc tactgccagc
     7141 gcaagttcgg cttccgcgcc ctggagaagt tcgaggacgt cttccgcggc tccaaagagc
     7201 gggtcaatct cgagagcgag gcgatggtcg acaagtacga gctgttcgcc aacgagaacc
     7261 aggacgcggt ctggctgcgc cgcctcttct cacccacctt catcgtctgg ctgaccgatt
     7321 cggcgccgga gaagttcgcc ttcgagctgg tcaacggcag gctctgctgc tacgtcagcg
     7381 gccacaagga aaaggccgac cagctcgaca ccgtgatggc cgccagcgtc gccgtcgcca
     7441 cccgcctgcg cgaagaggcg acgcagacca gctagcagcc ctcccggctt tttacgtcct
     7501 atggcacgtg caaaggcggg agcgcggtca gaacccggcg agcagggtgc cggcgttggc
     7561 gatcaggccg gcggcgatca tcagcaggac cgagggcacg aggacgaggg cgacgacgag
     7621 ctggatcttc ggcgcggcgc gggccgctcg ctcctcgacc gcccggcggc tgtcgcggag
     7681 cagtgccgtg gcctgacgac ggagctggtc ggcgaggggc gagccgaacc tgcggga
//
LOCUS       DASRIG010000383         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3577892, whole genome shotgun sequence.
ACCESSION   DASRIG010000383 DASRIG010000000
VERSION     DASRIG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577892"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..928
                     /locus_tag="VFB52_10255"
     CDS             227..928
                     /locus_tag="VFB52_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06759.1"
                     /translation="MGPPSLSRRRWLLLLPLLLAVALAVLLWPEPEPTALPAIDYNPP
                     RIELPPIDVDVPDPEVQVQGPSGSRGGEVIEKTTPPAPPPPPPDPEYKNKILHEHHEW
                     WLWDLNPWLMALAGLGAALAWQLSRVGSRLGWAGRALAVAALTGLATLAFVVGINPRA
                     GDGIYSRKPAEIERIDGVPEVIVRHIHIDHFHPPIDIAAHWLLLGVGLALVAVWTARP
                     LIWAGWKRRRAATPV"
     gene            1100..2512
                     /gene="pyk"
                     /locus_tag="VFB52_10260"
     CDS             1100..2512
                     /gene="pyk"
                     /locus_tag="VFB52_10260"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZO06760.1"
                     /translation="MDRRTKIVATVGPASWDQGVLEQMIDAGADVFRLNFSHAGPDQQ
                     AQTIETIRKAAENVGREVAVLGDLPGPKLRIGELRDDFAELETGMHVTLKPGVDEGDR
                     EQIPVPWSGVTDLRENQLVYLADGAIRLRVHEPESDGVDCEVEVGGTLSSHKGMNIPD
                     GAGLPAATAGDLGWVEFAVSHEVDLLAVSFVSSADDLIPVRERIAARGADIPLIAKIE
                     RPQAAHDIEAIVEAATGGIMVARGDLGIELPLAEVPGVQKRMIRAAGKRSKPSITATQ
                     MLASMVTARRPTRAEVTDVANAIYDGTDAIMLSEETAVGQHPVEAVRVMDRIARATEP
                     DLPYGEWLFSRTDQATADVADSISQSAVGAAYRLNLKALVVPTTSGRTARLISAHRPD
                     VPVLAISPRTETVRRLNLLFGVTCVLYEHETDVRSLLRDCARLAAHHGIAESGDLIAI
                     IAALPDQELGTNLFEVHRIP"
     gene            2557..3348
                     /locus_tag="VFB52_10265"
     CDS             2557..3348
                     /locus_tag="VFB52_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO06761.1"
                     /translation="MAGEGQPVRVEREASGLAVLTLDNPPLNLFDKAQIDALAAAIAE
                     LAAEPPRALLIRAEGRAVSAGVDVHVFDGLTPETGSALWTDLLVMIDQLEELPAPVIF
                     AAHALTLTAAFELALACDLIVATPSAKFGLVEKVVGLTPSMGGTQRLAERAGSGRARH
                     FVMSGELFGAEQMESWGVVNAIYPEDEFEARSRALAEELAAGPTKAHAMTKHILRRFR
                     EGGLPAADKVVRLEAADLFATEDLQNAVKTFLEHGGPGHATFEGR"
     gene            3368..3838
                     /locus_tag="VFB52_10270"
     CDS             3368..3838
                     /locus_tag="VFB52_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HZO06762.1"
                     /translation="MPEMPNVDFDAIAKGMAMAVPFAGHLNLEIAEISAGEAKVVLPR
                     RPELNNHVGSQHAGALFTAAETASGAAFVGAFAIRMGEITPLARSAEIDYKKIANGRI
                     TATATLGIEAADALATLDAEGKVEFPCEVVLTDESGTEVATATVHWHVRLKDGP"
     gene            3921..3994
                     /locus_tag="VFB52_10275"
     tRNA            3921..3994
                     /locus_tag="VFB52_10275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3955..3957,aa:Arg,seq:acg)
     gene            4185..>4838
                     /locus_tag="VFB52_10280"
     CDS             4185..>4838
                     /locus_tag="VFB52_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06763.1"
                     /translation="MRHLRWVAGFSSVLLAISAVPVLAEDRPEGSPAHTLPAQAEDFD
                     ISRLPDGHDVAVAIDKAEEEEEQREAWISSPEAIQQREASKLSYSGLSAAESEELLQA
                     TFAELLAQLDFDPARFLSDAQLLGTGEDPTAATVKDEGQRLVMDAGNVPVRTVNEDGR
                     RAKVDLSLEPTPEGWETKNALVDVRIPSSPEQPIGVGDNGVSIKLLGAEAQHAAQPFG
                     "
BASE COUNT          769 a         1694 c         1660 g          715 t
ORIGIN      
        1 cgagttcccg ttcgggggct atctgcccca gggcacgaac atccgggcgg cgcggctcgc
       61 ggcggtgccg gagttctgga aagcgtacgc agcgcgagcc ggggcgaccc tggcctggcg
      121 cggctgagcc atgggcggcc gagtctcacc cggctatcgc tccccgcggc ggatccgggc
      181 ccgcggtggc cgggcgatgc cgggcgggcg ggtcgggctg agcttggtgg ggccgccgtc
      241 gctcagccgc cgtcgctggc tcctgctcct gccgctgctt ttggcggtgg cgctggcggt
      301 gctgctctgg cccgagccgg agcccacggc gttgcccgcg atcgactaca acccgccgag
      361 gatcgaactg ccgccgatcg atgtggacgt cccggacccg gaagtccagg tccagggtcc
      421 ttcggggtcg cggggaggcg aagtgattga gaaaacgacg cccccggccc cgcctccgcc
      481 gccacccgac cccgaataca agaacaagat cctccacgaa caccatgaat ggtggctgtg
      541 ggatctgaac ccctggttga tggcgctggc tgggctcggt gccgcgctcg cctggcagct
      601 ttcgcgggtc gggagccgac tcggttgggc cggccgggcg ctggcggtcg cggcgctcac
      661 cggcttggcg acgctcgcct tcgtggtcgg gatcaacccg cgggcggggg acggcatcta
      721 cagccgcaaa ccggcggaga tcgagcgcat cgacggggtc ccggaagtga tcgtcaggca
      781 tatccacatc gaccacttcc acccgccgat cgatattgcc gcccactggc tgctgctggg
      841 agttggcctg gcgctggttg ccgtttggac tgcgcgccca ttgatctggg ccggctggaa
      901 gcggcggcgc gctgcgacgc ctgtctgatc tctaatcttt actaatccag tcaagaaaga
      961 gtccgttgcc gagaagcgca attagccctc caaacggggg tttcgaggcg gacttacaac
     1021 acatttaccg ggacgccttg ttccgtcccc tacagtcaaa agcgtgcact cgaacccgat
     1081 cgccaaagga ccggcccata tggacaggcg gacgaagatc gtcgccacgg tcggccctgc
     1141 cagttgggac cagggtgtgc tcgagcagat gatcgacgcg ggggccgatg tcttccggct
     1201 gaacttctct cacgcagggc ccgaccagca ggcgcagacg atcgagacga tccgcaaggc
     1261 ggccgagaac gtcgggcgcg aggtcgcggt gctcggcgac ctgccggggc cgaagctgcg
     1321 gatcggcgag ctgcgcgacg acttcgccga gctggagacg gggatgcacg tgaccctgaa
     1381 gccgggggtc gacgagggcg accgcgagca gatcccggtg ccctggtccg gggtcaccga
     1441 tctgcgcgag aaccagctcg tctacctcgc cgacggcgcg atccggctgc gcgtgcacga
     1501 gcccgagtcc gacggggtcg actgcgaggt cgaggtcggc ggcacgctct cttctcacaa
     1561 ggggatgaac atccccgacg gcgccggtct gcccgccgcg accgctggcg acctcggctg
     1621 ggtcgagttt gcggtctccc acgaagtcga cctgctcgcc gtctccttcg tctccagcgc
     1681 cgacgacctg atcccggtgc gcgagcggat cgcggcgcgc ggcgccgaca tcccgttgat
     1741 cgcgaagatc gagcggccgc aggcggccca cgacatcgag gcgatcgtcg aggcggcgac
     1801 cggcgggatc atggtcgcgc gcggcgacct cgggatcgaa ctgccgctcg ctgaggttcc
     1861 gggcgtgcag aagcggatga tccgcgccgc cggcaagcgc tcgaagccgt cgatcacggc
     1921 gacccagatg ctcgcttcga tggtgaccgc gcggcgcccg acccgggccg aggtgaccga
     1981 cgtcgccaac gcgatctacg acggcaccga cgcgatcatg ctctccgagg agaccgcggt
     2041 cggccagcac ccggtcgagg cggtgcgggt gatggaccgg atcgcgcgcg cgaccgagcc
     2101 cgacctccct tatggcgagt ggctgttcag ccgcaccgac caggcgaccg ccgacgtcgc
     2161 cgactcgatc tcccagagcg ccgtcggcgc cgcctaccgg ctcaacctga aagcgctggt
     2221 ggtgccgacc acgagcgggc gcacggcgcg gctgatctcc gcccaccgcc ccgacgtgcc
     2281 ggtgctggcg atcagcccgc ggaccgagac ggtgcgccgg ctcaatctcc tgttcggcgt
     2341 gacctgcgtg ctctacgagc acgagaccga cgtgcgcagc ctgctgcgcg actgcgcccg
     2401 gctcgccgcc caccacggga tcgccgagtc cggcgacctg atcgcgatca tcgcggcgct
     2461 gcccgatcag gaactgggaa ccaacctttt cgaggtccac aggatcccct gaggacccgc
     2521 ggcggcggtc aagcttcttg aagtagcctg ccggggatgg caggggaggg acaaccggtt
     2581 cgcgtcgagc gcgaggccag cggcctggcc gtgctgacgc tcgacaaccc gccgctcaac
     2641 ctcttcgaca aagcccagat cgacgccctg gcggcggcga tcgccgagct cgccgcggag
     2701 ccgccgcggg cgctgctgat ccgcgccgag gggcgcgccg tctcggccgg cgtcgacgtg
     2761 cacgtcttcg acgggctgac cccggaaacc gggtcggcgc tctggaccga cctgctggtg
     2821 atgatcgacc agctcgaaga gctgccggcg ccggtgatct tcgccgcgca cgcgctgacc
     2881 ctgaccgctg ccttcgagct ggcgctcgcc tgcgacctga tcgtcgcgac gccgagcgcc
     2941 aagttcggcc tggtcgagaa agtggtcggg ctgacgccgt cgatgggcgg cacgcagcgg
     3001 ctcgccgagc gggccggcag cggccgggcg cgccacttcg tgatgagcgg cgagctgttc
     3061 ggcgccgagc agatggagag ctggggcgtg gtcaacgcga tctaccccga ggacgagttc
     3121 gaggcgcgct cgcgggcgct ggccgaggag ctggcggccg ggccgaccaa ggcgcacgcg
     3181 atgaccaagc acatcctgcg ccgcttccgc gagggcgggc tgccggcggc cgacaaggtt
     3241 gtgcggctcg aagccgccga cctatttgcg accgaggacc tgcagaacgc ggtcaagacc
     3301 ttcctcgagc acggcgggcc cggccacgcc actttcgagg gccgctagct ccagctaggg
     3361 tttccggatg cccgagatgc cgaacgtcga cttcgacgcg atcgccaaag gaatggcgat
     3421 ggccgtgccc ttcgccgggc acctgaacct ggagatcgcc gagatctccg ccggcgaagc
     3481 gaaggtcgtc ctgccccggc gcccggagct caacaaccac gtcggcagcc agcacgccgg
     3541 cgccctcttc accgccgccg agaccgcctc cggcgctgcc ttcgtcggcg ccttcgcgat
     3601 ccgcatgggc gagatcaccc cgctcgcccg ctccgccgag atcgactaca agaagatcgc
     3661 caacggccgg atcacggcca ccgccaccct cggcatcgag gccgccgacg ccctcgccac
     3721 cctcgacgcc gaaggcaagg tcgagttccc ctgcgaagtc gtcctcaccg acgagtccgg
     3781 caccgaagtc gccacggcca cggtccactg gcacgtccgg ctcaaagacg gcccctgagc
     3841 cgcctcgacc gcgcaagacc cctagaattc cgcctcccgc gattcccgcg ccccacccgg
     3901 cgcgcgattc cgccgggcac gcgcccgtag ctcagccgga tagagcgtcg gtctacgaaa
     3961 ccgaaggtca caggttcgaa tcctgtcggg cgcgttgccg gtccggtcct ctgcggcctc
     4021 gagctgcgcg actcaagcga ccgagtttgc ggcgaattac agtcgattaa cgaagaaatc
     4081 cttttcttcg tgtagaaatc tgagcaaacc tcggccagga ggatgttttg ttcaatcgct
     4141 cgcgttgctc ggattcggtt cgtcaatccg gctggggtgc cggggtgagg caccttcgct
     4201 gggtcgccgg tttcagctcg gtcttgctcg ccatctcggc agttccggtc ctagctgagg
     4261 accggccaga gggctctcca gcgcacacgc tgccggccca ggctgaagac ttcgatatct
     4321 ctcggcttcc cgatggccac gatgtcgccg tagcgatcga caaggcagag gaggaagaag
     4381 aacagcgaga agcgtggatc tcctcgcccg aggcgatcca gcagcgcgag gcttcgaagc
     4441 tgagctactc cggcctttcc gccgcggaga gcgaggagct tctgcaggcc accttcgccg
     4501 agctgctggc gcagctcgac ttcgatccgg cccgcttcct cagcgacgct cagctgctgg
     4561 ggacgggcga agatccgacc gccgccacgg tcaaggacga gggtcagcgc ctggtcatgg
     4621 acgcaggcaa cgtcccggtg cggacggtca acgaggacgg tcggcgcgcc aaagtcgacc
     4681 tcagcctcga gcccacgccc gagggctggg agaccaagaa cgccttagtg gacgtgcgga
     4741 tccccagctc cccggagcag ccgatcgggg tcggcgacaa cggtgtctcg atcaagctcc
     4801 tgggtgccga agcccagcac gccgcgcagc ccttcggc
//
LOCUS       DASRIG010000384         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1498427, whole genome shotgun sequence.
ACCESSION   DASRIG010000384 DASRIG010000000
VERSION     DASRIG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498427"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFB52_10285"
     CDS             complement(<1..430)
                     /locus_tag="VFB52_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06764.1"
                     /translation="MKQLIWNRGRLWALALFAGLALAVGLSACGSSDSSDSSTTGSSE
                     SSETTESTAETATIDEAAAKAAGFPNYAEFSPPDLSTSRPESRIAFLMPETVQTLQRA
                     IVEGAEAAADKYNVELTVFDAGGYANVTKQVGQFETAIGQG"
     gene            complement(427..2019)
                     /locus_tag="VFB52_10290"
     CDS             complement(427..2019)
                     /locus_tag="VFB52_10290"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO06765.1"
                     /translation="MSAAAAAAPELEVRGVEKSFNGVRALKGVSLRIDRGEARALVGA
                     NGAGKSTLINVVCGAVQPDSGTVLVGGEEVAIRSPHVAIELGIGTVHQHDRLVPNLSV
                     AQNLQLGHEPTRTPARWLTKAPAGSLSALELVGLADRAGDLVEGMTLAQRQLVSIARA
                     VSLQPRLLILDEPTASLTPLETSYLIEVIDRLRRQGTALIYVTHRLQELTEVVDRVTV
                     LRDGCVVAERAPDVPEPELVELIAGEGVVANDEELVDERRRLARERGNLTASEPLLSV
                     SGLGDSAGAFEDVSFELRRGEVLGMVGLPDAGVTQLVETIAGARRSSSGTIALAEAPF
                     RARSPRAASRRGVGYLAGDRARCGVIPNSDVAETIALSALPRLSRGGLLSFRRLAAST
                     RRLLRQCQVDAADPGMPITALSGGNQQKALFARVLATEPRVVVCEDPTAGVDVAGRES
                     LYELVADLCERGGAVIWSGSDLREVATICDRVLVLWRGSVVASLERQELDERALMVAQ
                     FNQGQGEAPVGAPTPKPGGETG"
     gene            complement(2016..>2673)
                     /locus_tag="VFB52_10295"
     CDS             complement(2016..>2673)
                     /locus_tag="VFB52_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06766.1"
                     /translation="AATGVGLLNGVLIAICGLSPIVVTLAMGQALLGVALLLSPNGPI
                     QVFDPGYGELAASMAGPVPTLVIAAIVCLALGHLLLKRFSLGRYIYSVGGNETAAWLA
                     GVPTRSVKLTAYAIGGAFAGIAGILLSSRTGSGEATLGTTIMLEAYAAAFIGGVGFGT
                     GKGTVIGVALGALVLGVISNGIDLLGMNGNWQYIVSGGLIVFAIGFQKLAGKLNWSRS
                     "
BASE COUNT          382 a          951 c          965 g          375 t
ORIGIN      
        1 agccctggcc gatcgccgtt tcgaactggc cgacctgttt ggtcacgttc gcgtagccgc
       61 cggcgtcgaa cacggtcagc tcgacgttgt atttgtcagc cgccgcttcc gccccttcga
      121 cgatcgcccg ctgcagggtc tgcaccgttt ccggcatcag gaaggcgatg cgggattcgg
      181 gcctggaggt gctcaggtcc ggtgggctga attcggcgta gttcgggaag ccggccgctt
      241 tcgccgccgc ttcgtcgatc gtcgcggttt ccgcggtgct ttcggtcgtt tcgctgcttt
      301 cgctcgaccc ggtggtcgag gagtcgctgc tgtcagacga cccgcacgcg ctcaaaccga
      361 ccgcgagcgc gaggcccgcg aacagggcca acgcccagag tcgcccacgg ttccaaatca
      421 actgcttcat ccggtctctc ctcctggttt gggtgtggga gcgcccactg gggcctcccc
      481 ctgtccctgg ttgaactggg cgaccatcaa ggctcgctcg tccagttcct gccgctccag
      541 cgaggcgacc acggacccgc gccagagcac gagcacccgg tcgcagatcg tggccacctc
      601 gcgaagatcg ctgccgctcc agatcaccgc tccgccccgc tcgcagaggt cggcgacgag
      661 ttcgtagagg gactcgcgcc cggcgacgtc gacgcccgcg gtcgggtcct cgcagacgac
      721 gacgcgcggc tcggtcgcga gcacccgcgc gaacagcgcc ttctgctggt tgccgccgct
      781 gagcgcggtg atcggcatcc ccggatcggc ggcgtcgacc tggcattggc ggagcaggcg
      841 gcgggtcgaa gccgccagcc ggcggaagga cagcaggccg ccgcggctca gccgcggcag
      901 ggccgagagg gcgatcgttt cggcgacgtc ggagttgggg atcaccccgc acctggcgcg
      961 gtcgccggcc aggtacccga ccccgcggcg cgaggcggcg cgggggctgc gggcgcggaa
     1021 gggggcttcc gcgagggcga tcgtgccgga gctcgaacga cgcgccccgg cgatcgtctc
     1081 gaccagctgg gtgacgccgg cgtcgggcag gccgaccatg ccgagcacct cgccccggcg
     1141 cagctcgaag ctgacgtcct cgaaggcgcc ggccgagtcg ccgaggccgc tgacgctgag
     1201 cagcggttcg gaagcggtca ggttgccgcg ctcgcgggcg agccggcggc gctcgtcgac
     1261 cagctcctcg tcgttggcga cgacgccctc gccggcgatc agctcgacca gctcgggctc
     1321 gggcacgtcg ggggcgcgtt cggcgaccac gcagccgtcc cgcaacaccg tcacccggtc
     1381 gacgacctcg gtcagctcct ggaggcggtg ggtgacgtag atgagcgcgg tcccctggcg
     1441 gcgcaggcgg tcgatcacct cgatcaggta gctggtctcc agcggggtca gcgaggcggt
     1501 cggctcgtcg aggatcagca gccgcggctg cagggagacc gcccgggcga tcgagaccag
     1561 ctggcgctgg gcgagggtca tcccctcgac caggtcgccg gcgcgatcgg ccaatccgac
     1621 cagctccagg gccgagagcg agcccgcggg ggccttggtc agccaccgcg ccggggtccg
     1681 ggtcggctcg tggccgagct ggaggttctg ggcgacgctg aggttgggga ccagccggtc
     1741 gtgctggtgg acggtgccga tgccgagctc gatcgccacg tgcggggagc ggatcgcgac
     1801 ctcctcgccg ccgaccagca cggtcccgga gtcgggctgc accgccccgc agacgacgtt
     1861 gatcagggtc gacttgccgg ccccgttggc gccgactagg gcccgcgcct cgccgcgatc
     1921 gatgcgcagc gagacgccct tcagcgcccg cacgccgttg aacgacttct cgaccccgcg
     1981 gacctccagc tcgggcgcgg cggcggccgc ggcgctcacg agcggctcca gttgagcttt
     2041 ccggccagct tctggaagcc gatcgcgaag acgattaggc cgccgctgac gatgtactgc
     2101 cagttgccgt tcatccccag caggtcgatc ccgttcgaga tcaccccgag gacgagcgcg
     2161 ccgagcgcga ccccgatcac ggtgcccttg ccggtgccga aaccgacgcc gccgatgaac
     2221 gcggcggcgt aggcctcgag catgatcgtc gtgcccagcg tcgcttcgcc ggagccggtc
     2281 cgcgaggaga gcaggatccc ggcgatcccg gcaaaggcgc cgccgatcgc gtaggcggtc
     2341 agcttcaccg agcgagtcgg gaccccggcc agccaggccg cggtctcgtt gccgccgacc
     2401 gagtagatgt agcggccgag gctgaagcgc ttcagcagca ggtggccgag cgcaaggcag
     2461 acgatcgcgg cgatcaccag ggtcggcacc gggccggcca tcgacgccgc gagctcaccg
     2521 tagccggggt cgaagacctg gatcgggccg ttcggcgaga gcagcagggc gaccccgagc
     2581 agcgcctgcc ccatcgccag ggtgacgacg atcggcgaga ggccgcaaat cgcgatcagg
     2641 accccgttca gaagcccgac cccggtcgcc gcg
//
LOCUS       DASRIG010000385         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2261166, whole genome shotgun sequence.
ACCESSION   DASRIG010000385 DASRIG010000000
VERSION     DASRIG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261166"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="pstA"
                     /locus_tag="VFB52_10300"
     CDS             <1..889
                     /gene="pstA"
                     /locus_tag="VFB52_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZO06767.1"
                     /translation="CVAIYTWSRAVEGPRRAFDRTMTMAIAAAFGLAMIPLASLLFEV
                     ISRGLPGLSLEFLTEDARGKIGGGGAAHALVGTLVITGVAAIISVPIGIMAAIYLTEY
                     GTGKLSKALTFFVDVMTGIPSIVAGLFAYALFELFLGPGIRLGVMGSVALSVLMIPIV
                     IRSAEEVLKIVPNHLREAAYALGTPKWKTITKIVLPTAFAGLVTGVMLAVARIIGETA
                     PLLVTTGVIDSINSDPFDGRMQNLAVYAYSEYRSPGIDKAASYDRAWAAALLLIAIVM
                     ILNVVARLVYRRYKTEIHR"
     gene            916..1815
                     /gene="pstB"
                     /locus_tag="VFB52_10305"
     CDS             916..1815
                     /gene="pstB"
                     /locus_tag="VFB52_10305"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZO06768.1"
                     /translation="MGSSEKKEGATESGSEQLSQTAAAAATTTTGTTATASAAGVAKT
                     IEVRDVNLFYGDFHAVEDVNLTIQPNKVTALIGSSGCGKTTFLRSLNRMHELTPGARV
                     EGHITLDGEDMYARGVDPVRVRRLVGMVFQQPNPFPTMSIYDNVASGLNLNAGKIKKS
                     QRDAVVERSLRGANLWEEVKDRLDKPGLGLSGGQQQRLCIARAIAVEPEVLLMDEPCS
                     ALDPVATLAIEDLIGELKSRYTIVIVTHNMQQAARASDLTAFFNLEESGKPGRLVEVG
                     ETEMIFTNPTEKATEDYVSGRFG"
     gene            1836..2522
                     /gene="phoU"
                     /locus_tag="VFB52_10310"
     CDS             1836..2522
                     /gene="phoU"
                     /locus_tag="VFB52_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZO06769.1"
                     /translation="MPEVAARAEYVEELTNLEQSALGGLDLVNDALTRTLEAIEHQDI
                     ELAQLVVADDDRIDGRYLEIHQSLITLLATQSPVATDLRLIASLLHVLKNIERMGDQC
                     VNICKVIPLSGNEPPADEEMVKRILAMGKQTRVLIAQAKRSFLERDVDQARDLVRQDD
                     VVDNLNRECFYLALAIGDDADRREWAMTMLLTARAIERIADNAVDIGEQVAFVVTGLF
                     REFEDASHPA"
     gene            2581..3492
                     /locus_tag="VFB52_10315"
     CDS             2581..3492
                     /locus_tag="VFB52_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06770.1"
                     /translation="MLCAAIDIGSNTTRVLVAEPVDGQLKKVMEQRAYTRINKALDGD
                     GAIPAEKIAEVGEVVATQVRLARELGAQEIRAVATAAVRDSTNGEQAAAAVSRAAGVE
                     VEVLSEQEEGRLAFLGATKALGHPAEGRIGVVDVGGGSTEVILGTVAGGVEKVQSWQV
                     GSGVLADELISSDPPSAAEIRKVRDRIDDIFEGVEIEHPAQAVAVGGSATSLRRLVGA
                     VLEYETLERGIRVLCGDPAAEVAKRFELDPVRVRILTTGVLILEKVSELLGQPLQIGK
                     GGLREGIILDLLNNGRSNGRPPIRLVA"
     gene            3613..>4499
                     /locus_tag="VFB52_10320"
     CDS             3613..>4499
                     /locus_tag="VFB52_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HZO06771.1"
                     /translation="MTSRVDMHCHSTASQLSRLGVQRSLGLPECATPPEEVYELAKRR
                     GMDFVTITDHDTIEGALALADRPDCFVSEELTARFAGEPQAVHVLCYGITPGDHEWLQ
                     AHSGDVEACAAYLRENEITCALAHPFFDVGAPLTRRHRRRLAELFPIWEVRNGSRAAE
                     LNMPAVVYIETNDGTGIGGSDDHAGVDIGRTFTEVPDARTPEEFLQQIRAGHASAGGT
                     QGSAAKWAHAAMALATRALGEGASESTPPDPGAVLKMAERVIREGEAREGKVATDIGP
                     ADARALLEAWLGSVGLELRG"
BASE COUNT          754 a         1564 c         1524 g          657 t
ORIGIN      
        1 ctgcgtcgcc atctacacct ggtcgcgcgc cgtcgagggg ccgcgccgcg ccttcgaccg
       61 gacgatgacg atggcgatcg cggcggcctt cggactggcg atgatcccgc tcgcctcgct
      121 gctcttcgaa gtgatcagcc gcggactgcc cggcctcagc ttggaattcc tcaccgagga
      181 cgcgcgcggc aagatcggcg gcggtggcgc cgcgcacgcg ctggtcggga cgctggtgat
      241 caccggcgtg gcggcgatca tctcggtccc gattgggatc atggcggcga tctacctgac
      301 cgagtacggg accgggaaac tcagcaaggc gctgaccttc ttcgtcgacg tgatgaccgg
      361 gatcccctcg atcgtcgccg gcctcttcgc ctacgcgctg ttcgagctct tcctcgggcc
      421 gggcatccgg ctcggcgtga tgggctcggt ggcgctctcg gtgctgatga tcccgatcgt
      481 gatccgcagc gccgaggagg tgctgaagat cgtcccgaac cacctgcgcg aagccgccta
      541 cgcgctgggc acgccgaagt ggaagacgat caccaaaatc gtcctgccga ccgcattcgc
      601 cggcctggtc accggtgtga tgctggcggt ggcccggatc atcggcgaga ccgcgccgct
      661 cctggtcacc accggcgtga tcgactcgat caactccgac ccgttcgacg gccgcatgca
      721 gaacctcgcc gtctacgcct acagcgagta ccgctcgccc gggatcgaca aagccgcttc
      781 ctacgatcgc gcctgggccg ccgccctact cttgattgcg atcgtgatga ttctcaacgt
      841 cgtcgctcgc ctcgtctacc gccgttacaa aaccgaaatc caccgctgat ggaggggaag
      901 aaagtgagtg ccaccgtggg ttcctcagag aaaaaagagg gcgcgacgga gtccggctcc
      961 gagcagctct cccagaccgc tgccgctgca gccaccacga ctaccggcac gaccgcgacc
     1021 gcttccgccg ctggagtggc gaagacgatc gaggtgcgcg acgtcaacct cttctacggc
     1081 gacttccacg cggtcgaaga cgtcaacctg acgatccagc cgaacaaggt gacggcgctg
     1141 atcggttcct cgggctgcgg caagaccacc ttcctgcgct cgctcaaccg gatgcacgag
     1201 ctgacgccgg gggcgcgggt cgagggccac atcacgctcg acggcgagga catgtacgcg
     1261 cgtggggtgg acccggtgcg ggtgcggcgc ctggtcggga tggtcttcca gcagccgaac
     1321 ccgttcccga cgatgtcgat ctacgacaac gtcgcctccg gcctcaacct caacgccggc
     1381 aagatcaaga agtcccagcg ggacgcggtg gtcgagcgca gcctgcgcgg cgccaacctc
     1441 tgggaagagg tcaaggaccg gctcgacaag cccggcctcg gcctctccgg cggccagcag
     1501 cagcggctct gcatcgcccg cgcgatcgcg gtcgagcccg aggtgctgct gatggacgag
     1561 ccctgctcgg cgctcgaccc ggtggcgacg ctggcgatcg aggacctgat cggcgagctg
     1621 aagagccgct acacgatcgt gatcgtcacc cacaacatgc agcaggcggc gcgggccagc
     1681 gacctgaccg ccttcttcaa cctcgaagag tcgggcaagc cgggccgcct ggtcgaggtg
     1741 ggggagaccg agatgatctt tacgaacccg accgagaaag cgaccgagga ctacgtcagc
     1801 ggccgcttcg gctagccgaa gggtaggagg ggaccatgcc ggaagtcgcc gcaagagccg
     1861 aatacgtcga ggaactgaca aacctcgagc agagcgccct gggcgggctc gacctggtca
     1921 acgacgcgct gacccggacg ctggaggcga tcgagcacca ggacatcgag ctggcccagc
     1981 tggtcgtcgc cgacgacgac cggatcgacg gccgctacct ggagatccac cagagcctga
     2041 tcacgctgct ggcgacccag tcgccggtcg cgaccgacct gcgcctgatc gcctcgctgc
     2101 tgcacgtgct gaagaacatc gagcggatgg gcgaccagtg cgtgaacatc tgcaaggtca
     2161 tcccgctctc cggcaacgag ccgccggccg acgaggagat ggtcaaacgc atcctcgcga
     2221 tgggcaaaca gacgcgggtc ctgatcgcgc aggccaagcg ctccttcctc gagcgcgacg
     2281 tcgaccaggc gcgcgacctg gtccgccagg acgacgtcgt cgacaacctc aaccgcgagt
     2341 gcttctacct ggcgctggcg atcggcgacg acgccgaccg ccgcgaatgg gcgatgacga
     2401 tgctgctgac cgcacgggcg atcgagcgga tcgccgacaa cgccgtcgac atcggcgagc
     2461 aggtcgcgtt tgtcgtcacc ggcctgttcc gggagttcga ggacgcttcg cacccggcgt
     2521 aacccccgcc cccgcgcgtg tcagatccga cgcgtctggg aacattcagg ctgaagccgc
     2581 atgctctgtg ccgccataga tatcgggtcc aacaccacgc gagtgctcgt tgccgagccg
     2641 gttgacgggc agttgaagaa ggttatggag cagcgcgcct acacccggat caacaaagcg
     2701 ctcgacggcg acggggcgat cccggcggag aagatcgccg aggtcggcga ggtcgtcgcg
     2761 acccaggtgc gcctggcccg cgagctcggc gcgcaggaga ttcgcgcggt cgcgaccgcg
     2821 gcggtgcgcg actcgaccaa cggtgagcag gccgccgcgg cggtctcgcg cgcggccggg
     2881 gtcgaggtcg aagtcctcag cgagcaggag gaggggcggc tggccttcct cggcgcgacc
     2941 aaagcgctcg ggcacccggc ggagggccgg atcggcgtcg tcgacgtcgg tggcggctcg
     3001 accgaggtga tcctcggcac ggttgccggc ggggtcgaga aggtgcagtc ctggcaggtc
     3061 ggctccggcg tcctcgccga cgagctgatc tcctccgatc caccctcggc ggccgagatc
     3121 cgcaaggtcc gcgaccgcat cgacgacatc ttcgaggggg tcgagatcga gcaccccgcc
     3181 caggccgtag cggtcggggg ttccgccacc agcctgcgcc gcctggtcgg cgccgtgctc
     3241 gagtacgaga ccctggagcg cggcatccgc gtgctctgcg gcgaccccgc cgccgaggtc
     3301 gccaaacgct tcgagctcga cccggtccgg gtccgcatcc tcaccaccgg cgtgctgatc
     3361 ctcgagaagg tctccgagct gctcggccag ccgctgcaga tcggcaaggg cggtctgcgc
     3421 gaggggatca tcctcgatct cctcaacaac ggccggagca acggccgccc gccgattcgc
     3481 ctcgtcgcct agccctctcg ccctctcggc ggtcaccgga cgttcacgcc ccgtttgcag
     3541 ggtgttcaca agtttcccct gcaaatcccg aagatgtggg tgacaacgga atagatgaga
     3601 gggagagcac cgatgacttc ccgtgttgac atgcattgcc actccaccgc ttcgcagctt
     3661 tcgcgactgg gggtccagag gtcgctgggt ctgcccgagt gcgcgacgcc tcccgaggag
     3721 gtctacgaac tggccaagcg gcggggtatg gacttcgtca cgatcaccga tcacgacacg
     3781 atcgaggggg cgctggcgct ggccgaccgc cccgactgct tcgtctccga ggagctgacc
     3841 gcccgcttcg ccggcgagcc ccaggcggtt cacgtcctct gttacgggat cactcccgga
     3901 gaccacgagt ggctgcaggc acattccggc gatgtagagg cctgcgccgc atacctgcgc
     3961 gagaacgaga tcacgtgcgc gctcgcgcac cccttcttcg acgtcggcgc gccgctcaca
     4021 cggcgccacc ggcgtcgcct ggcggagctc ttcccgatct gggaggtccg caacggttcc
     4081 cgggccgccg agctgaacat gcccgccgtc gtctacatcg agaccaacga cggcaccggc
     4141 atcggcggtt ccgacgacca cgccggcgtc gacatcggcc gcaccttcac cgaggtgccc
     4201 gatgcgcgga cgccggaaga gtttctgcag cagatccgcg cgggccacgc gagcgccggc
     4261 ggcactcagg gcagcgccgc caagtgggcc cacgcggcga tggcgctcgc cacccgcgcg
     4321 ctcggcgagg gcgcgagcga gagcacgccg ccggaccccg gcgcggtgct gaagatggcc
     4381 gaacgggtga tccgcgaggg cgaggcgcgc gagggcaagg tggcgaccga catcggcccc
     4441 gcggacgccc gcgccctgct cgaggcctgg ctcggcagcg tcggcctgga gctgcgcgg
//
LOCUS       DASRIG010000386         7002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2798916, whole genome shotgun sequence.
ACCESSION   DASRIG010000386 DASRIG010000000
VERSION     DASRIG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798916"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1223)
                     /locus_tag="VFB52_10325"
     CDS             complement(42..1223)
                     /locus_tag="VFB52_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZO06772.1"
                     /translation="MALLGATVALLALAAAPAGAATGGAGEEEEAPAAGKSPFDRHGM
                     WIWYVSKSEGGSIPAMIRRAKAAGIGTVYVKSADGSNVWSQFNRQLVQRLHAGGIDVC
                     AWQFVYGDRPAAEAQAAAISIARGADCFVIDAEGDYEGKYAAADLYTRKLRAAAGESY
                     PIGLAGFPYVDYHPSYPYSVFMGPGGATFNTPQMYWKTIGVSVRTIYEHTYLYNRIWG
                     HPIYPLGQTYEGPGNAQLKLFRRFAASYGQAPNWWDWQETDNREWGALAARSATQPVA
                     GYRPITEHPLLKKGAKGDMVVWAQMHLVGAGQEVPITGIFGRMTIAAVRTFQEEKGMK
                     VDGQIGTDTWKALLAYAPERPRWYSGARTSGAGRALPSTRPLSASLPAKAYEIPPGPG
                     R"
     gene            1385..2704
                     /locus_tag="VFB52_10330"
     CDS             1385..2704
                     /locus_tag="VFB52_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO06773.1"
                     /translation="MRIAIVGTGISGLVAAHGLHRRHEITVYEAAPRLGGHTNTVRVE
                     DPDGELSIDTGFIVFNDRNYPNFEALLAELGVASQPSHMGFSVADGRGRFEYSGTPWG
                     LFGRPSHLASPRFWRMLRDWRRFNREAQGLIGMNGTAPSLGTWLEQNNFSRQFIDRLI
                     VPQAAAVWSADPEQMWSFPASFMAEFFDNHGMYSLRDRPQWRTVRGGSRRYVEAISAP
                     WRHRVRLGAPVRRIERGPGGVRIEAEGCASERFDEVVIATHSDQALAMLADPSRAERE
                     VLGAIPYQRNEAVLHTDRSLMPRRRPVWSSWNFHLGEPPAPGAAPRGSTVTYWMNNLQ
                     RLPGSRDYFVTLNRGGEIEPERVIRRFEYDHPTYTREGVAAQARHAEISGVRRTHFCG
                     AYWGWGFHEDGVVSGLRVVERIAGAGRGAVAPPPSPPASADAEPLAA"
     gene            2701..3552
                     /locus_tag="VFB52_10335"
     CDS             2701..3552
                     /locus_tag="VFB52_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HZO06774.1"
                     /translation="MTATARRPAFVAQRATNAGRSAVYEGWVRHRRFEPVEHSFRYRL
                     FLMYLDLGEVPEVLDPYPGWSARRPAPARFQRADFMGDPSRPLAECARELVAAETGAR
                     PGGPVRLLTGLRYFGHSFNPVSFYYCFDPAGERVEAVVADVENIPWGERHPYVLARGE
                     RTGTVLADEMDKTLHVSPLMGMDQTYAFRASEPGERLAVHIESRPRAGADGAAPTAAK
                     SFDATLSLRRRELSRPLLSRLLARYPAMSLQVVAKIYAQSARLKLKGARYFPHPQGSR
                     PKGFIAP"
     gene            3549..4736
                     /locus_tag="VFB52_10340"
     CDS             3549..4736
                     /locus_tag="VFB52_10340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZO06775.1"
                     /translation="MRDALSRTAVGAVLGRVSAGRIEVVEDDRVRLYGPADSDLKATV
                     RINDPAAWRGPLRGSVGLGETYVDGLWETDDLVALIQIAARELHELDGLRGAVARPRG
                     ALHKLRGLVPENTKPGARANISAHYDLGNDLFAAFLDERMMYSCAYYPDESASLEDAQ
                     VAKLDRICERLQLGPENHLLEIGTGWGGMAIHAARRSGCRVTTTTISRAQHELATQRV
                     REAGLEDQITVLLKDYRDLEGRYDRLVSVEMIEAVGWQYFDEYFRRCDRLLSDDGAML
                     LQAITIDDRIYEVEKAAKSFANTHVFPGGCLPSEGLIANCLSRVTDMRQVWIDDITDH
                     YPPTLATWRERFFVAWEKLRERGYDERFRRLWDFYLSSSEAGFRERRISDIQALFAKP
                     GWR"
     gene            4733..5512
                     /locus_tag="VFB52_10345"
     CDS             4733..5512
                     /locus_tag="VFB52_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO06776.1"
                     /translation="MSENGAGPGGAGEPLLLIHGLGGSTGVWAPVLPLLAGERQVVAL
                     DLPGFGKAPPLPDGDEPTAARMAEVLHEQCLELGVERPHVAGNSLGAWTALEMGRFGW
                     ARSVTALSPAGLWRNTSPARRSPVRVWGNRLRPLVSAALAVKPLRERALTTFVGHPER
                     LPTAAGRELVLGWLTSPGYDAANRAMRSHLFDPDGYPEDVPVTIAWGELDRLVGPPKP
                     ERRPAGARFLTLPGVGHTPMWDDPELVARTLLEGSRTKERL"
     gene            5509..6258
                     /locus_tag="VFB52_10350"
     CDS             5509..6258
                     /locus_tag="VFB52_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO06777.1"
                     /translation="MIVERTENPMWLSNAYLVADPERGTGVLIDGNDDLDPLLEKAER
                     EGIEITHVLVTHPHPDHVAGLREAREKLGNPPLVAHAETAAELDDEVAVILADGDKLD
                     ANGIEVEAIYTPGHAAGHLALLVNGTDLFTADVLFKGTVGGTMAPGASGFDDLQASVM
                     RLMELPPETVVHPGHKEPTTIGEQFAENPFVRIWRGLDSTGEEEVTVWDRPATLKLWA
                     PDYDGGNKAWIVFGDSGEEAIVGGSQVVRDL"
     gene            complement(6330..>7002)
                     /locus_tag="VFB52_10355"
     CDS             complement(6330..>7002)
                     /locus_tag="VFB52_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06778.1"
                     /translation="EPRRTPMTHTAPTMTTKFARRLGFALALLAFAAAIVAPASGMAA
                     APVKFGSQLNPTVQPSNGSPGLQCEPGTLTPCTMVQNEAYGRPDGGEFAPKTGTIKRI
                     RIIAADAGSFRPQIATVKHTTSTTLGDTKAKVTYTGPKLSFTGQSEANYESGKYKVET
                     FNVNIPVKKGQQLALRGNVASMVRCSSGGHNTLIFTPALAKGGALQGATDANGCWILM
                     EAVIR"
BASE COUNT          994 a         2480 c         2512 g         1016 t
ORIGIN      
        1 ccggctcgtc accaagttcg ccgactagcg cgtcggcgcg atcagcggcc ggggccgggc
       61 gggatctcgt aggccttcgc gggcaacgag gccgagagcg gacgcgtcga gggcagcgcg
      121 cggccggcgc cactggtgcg ggcgccggag taccagcgcg ggcgttcggg ggcgtaggcg
      181 agcagcgctt tccaggtgtc ggtgccgatc tggccgtcga ccttcatccc cttctcttcc
      241 tggaaggtgc gcaccgcggc gatggtcatg cggccgaaga tcccggtgat cggcacttct
      301 tggccggcac cgacgaggtg catctgcgcc cagacgacca tgtcgccttt ggcgcctttc
      361 ttcagcagcg ggtgttcggt gatcgggcgg tagccggcga ccggctgggt cgcgctgcgc
      421 gcggccagcg ccccccactc gcggttgtcg gtctcctgcc agtcccacca gttgggcgcc
      481 tggccgtagc tcgcggcgaa gcggcggaac agcttcagct gcgcgttgcc ggggccttcg
      541 taggtctggc cgagcgggta gatcgggtgg ccccagatcc ggttgtagag gtaggtgtgc
      601 tcgtagatcg tgcggaccga gacgccgatc gtcttccagt acatctgcgg ggtgttgaag
      661 gtggcgccgc ccgggcccat gaagaccgag taggggtagg acgggtggta gtcgacgtag
      721 gggaagccgg cgaggccgat cgggtagctc tccccagcgg cggcgcgcag cttgcgcgtg
      781 tagaggtcgg cggcggcgta cttgccctcg tagtcgccct cggcgtcgat cacgaagcag
      841 tcggcgccgc gagcgatcga gatcgccgcc gcctgtgcct cggccgccgg ccgatcgccg
      901 tagacgaact gccaggcgca gacgtcgatt ccgcccgcgt gcaggcgctg gacgagctgg
      961 cggttgaact gcgaccagac gttgctgccg tcggccgact tcacgtagac ggtgccgatc
     1021 ccggccgcct tggcgcgccg gatcatcgcc gggatgctgc cgccctcgga cttcgagacg
     1081 taccagatcc acatcccgtg ccggtcgaag ggcgatttgc cggcggccgg cgcttcttcc
     1141 tcttcgccgg cacccccggt cgccgcgccc gccggggcgg ccgcgagcgc gagcagcgcg
     1201 acggtcgccc cgagcagggc caaccagcgc cgcgtctgaa accttcgcgt cctcacttcg
     1261 ccgtcaacac cttcgcgggc cgcgcgttgc gacgcggcgc ccaaggcggg tgcgcaagat
     1321 cgcgtgcgag ccggtcaggg gtcccgatgc aaccgatcgg gcctgcgctc cgtactgggg
     1381 gtcgatgagg atcgcgatcg taggcaccgg aatcagcggc ctcgtggccg cgcacgggct
     1441 gcaccggcgg cacgagatca ccgtctacga ggcggcgccg cggctcggcg ggcacacgaa
     1501 cacggtgcgg gtcgaggacc ccgacggcga gctctcgatc gacaccgggt tcatcgtctt
     1561 caacgaccgc aactacccca acttcgaagc gctgctcgcc gaactgggcg tcgccagcca
     1621 gccctcgcac atgggcttct cggtcgccga cgggcgcggg cgcttcgagt actcgggcac
     1681 gccctggggg ctcttcggcc gcccctccca cctggccagc ccgcgcttct ggcggatgct
     1741 gcgcgactgg cgccgcttca accgcgaggc gcaggggctg atcgggatga acgggaccgc
     1801 gccctcgctc ggcacctggc tcgagcagaa caacttctcc cgccagttca tcgaccggct
     1861 gatcgtcccc caggccgccg ccgtctggtc cgccgacccc gagcagatgt ggagcttccc
     1921 ggccagcttc atggccgagt tcttcgacaa ccacggcatg tacagcctgc gcgaccggcc
     1981 ccagtggcgg accgtgcgcg gcggctcgcg gcgctacgtc gaggcgatca gcgcgccctg
     2041 gcgccaccgg gtgcggctgg gtgcgccggt gcggcggatc gagcgcggcc ccggcggcgt
     2101 gcggatcgag gccgagggct gcgccagcga gcgcttcgac gaggtcgtga tcgcgaccca
     2161 ctccgaccag gcgctggcga tgctcgccga cccgagccgg gccgagcgcg aggtgctcgg
     2221 cgcgatcccc taccagcgca acgaagcggt cctgcacacc gaccgctcgc tgatgccgcg
     2281 ccgccgcccg gtctggtcga gctggaactt ccacctcggc gagccgcccg cgcccggcgc
     2341 ggcgccacgt ggctcgacgg tcacctactg gatgaacaac ctgcagcggc tgcccggcag
     2401 ccgcgactac ttcgtgaccc tgaaccgcgg gggagaaatc gagcccgagc gggtgatccg
     2461 ccggttcgag tacgaccacc cgacgtacac gcgcgagggc gttgccgccc aggcgcgcca
     2521 cgccgagatc agcggcgtgc gtcgcaccca cttctgcggc gcgtactggg gatggggatt
     2581 tcacgaggat ggcgtcgtca gcggccttag agtcgttgag cggattgcgg gtgcggggcg
     2641 tggggcggtg gctccccctc cgtccccacc cgcgtccgcc gacgccgaac ccctggccgc
     2701 atgaccgcca cggcgagacg ccctgcattt gtcgcccaga gggcgacaaa tgcagggcgg
     2761 tcggcggtct acgaggggtg ggtgcggcat cggcggtttg agccggtgga gcactcgttt
     2821 cggtatcggc tcttcttgat gtacctggac ctcggggagg tgccggaggt gctcgatccg
     2881 tatccgggct ggtcggcgcg gcggccggcg ccggcgcgct tccagcgggc cgacttcatg
     2941 ggcgacccga gccggccgct ggccgagtgc gcgcgcgagc tggtcgcggc cgagaccggc
     3001 gcgcggcctg gcggcccggt ccgcctgctc accggcctgc gctacttcgg ccactccttc
     3061 aacccggtca gcttctacta ctgcttcgac cccgctggcg agcgggtcga ggcggtcgtc
     3121 gccgacgtcg agaacatccc ctggggcgag cggcaccctt acgtacttgc gcgcggcgag
     3181 cggacgggta cggtgttggc ggacgagatg gacaagaccc tccacgtctc ccctctgatg
     3241 ggtatggatc agacctacgc cttccgcgcc agcgaacccg gcgagcgcct cgccgtccac
     3301 atcgagtcgc gcccgcgcgc cggcgccgac ggcgccgcac ccaccgccgc caagtccttc
     3361 gacgcgaccc tcagcctgcg ccgccgcgag ctctcgcggc cgctgctgtc acggctgctc
     3421 gcccgctacc cggcgatgtc cctgcaggtg gtggcgaaga tctacgcaca gtcggcgcgg
     3481 ctgaagctga agggggcgcg ctacttcccg cacccacagg ggagccgccc gaaagggttc
     3541 atcgcgccgt gagggacgcg ctctcccgga ccgcggtcgg cgcggtgctg ggccgggtca
     3601 gcgccggccg gatcgaagtc gtcgaagacg accgcgtgcg cctctacggc cccgccgact
     3661 ccgacctcaa ggcgacggtg cggatcaacg atccggccgc ctggcgcggg ccgctgcgcg
     3721 gcagcgtcgg gctcggcgag acctacgtcg acgggctctg ggagaccgac gacctcgtcg
     3781 ccctgatcca gatcgccgcc cgcgagctgc acgagctcga cggcctgcgc ggcgccgtcg
     3841 cccgtccccg cggcgccctt cacaagctgc gcgggctggt ccccgagaac accaagcccg
     3901 gcgcccgcgc gaacatctcc gcccactacg acctcggcaa cgacctcttc gccgccttcc
     3961 tcgacgagcg gatgatgtac agctgcgcct actacccgga cgagagcgcc tcgctggagg
     4021 atgcgcaggt ggcgaagctc gaccggatct gcgaacggct gcagctcggc cccgaaaacc
     4081 acctgctcga gatcggcacc ggctggggcg ggatggcgat ccacgccgcc aggcgcagcg
     4141 gctgccgggt gacgacgacg acgatctccc gcgcccagca cgagctggcg acccagcgcg
     4201 tgcgcgaggc cgggctcgaa gaccagatca cggtcctgct caaggactac cgcgacctcg
     4261 agggccgcta cgaccggctg gtctcggtgg agatgatcga ggcggtcggg tggcagtact
     4321 tcgacgaata cttccgccgc tgcgaccggc tgctcagcga cgatggcgcg atgctgctgc
     4381 aggcgatcac gatcgacgac cgcatctacg aggtcgagaa ggcggcgaag agcttcgcca
     4441 acacgcacgt cttcccgggc ggctgcctgc cctctgaggg gctgatcgcc aactgcctct
     4501 cgcgcgtgac cgacatgcgc caggtctgga tcgacgacat caccgaccac tacccgccga
     4561 ccctcgccac ctggcgcgag cgcttcttcg tcgcctggga gaaactgcgc gagcgcggct
     4621 acgacgagcg cttccgccgg ctctgggact tctacctgag ctcctcggag gcgggcttcc
     4681 gcgagcggcg gatcagcgac atccaggctc tgttcgcgaa accgggttgg cggtgagcga
     4741 aaacggggcc gggccgggcg gcgccggcga gccgctgctg ctgatccacg ggctcggcgg
     4801 cagcaccggg gtttgggccc cggtcctgcc cctgctcgcg ggagagcggc aggtggtcgc
     4861 actcgacctg ccgggcttcg gcaaggcgcc gccgctgccc gacggcgacg agccgaccgc
     4921 ggcgcggatg gccgaggtcc tgcacgagca gtgcctggag ctcggggtcg agcgcccgca
     4981 cgtcgccggc aactcccttg gcgcctggac ggcgctcgaa atgggccgct tcggctgggc
     5041 taggtcggtc accgccctct cccccgccgg cctttggcgc aacacctcac cggcgcggcg
     5101 cagccccgtc cgagtctggg gcaaccggct gcgcccgctg gtttcggcgg cgctggcggt
     5161 gaagccgctg cgcgagcggg cgctgacgac cttcgtcggc caccccgagc gtttgcccac
     5221 cgccgccggc cgcgaactgg tcctcggctg gctcacctcg ccgggctacg acgccgccaa
     5281 ccgggcgatg cgcagtcacc tcttcgaccc ggacggctac cccgaggacg tgccggtaac
     5341 gatcgcctgg ggcgagctcg accgcctggt cggcccgccg aagcccgagc gccgcccggc
     5401 cggcgcccgc ttcctcaccc tgcccggggt cggccacacg ccgatgtggg acgacccgga
     5461 gctggttgcg cgtacgctcc tggaaggaag ccgaaccaag gagagacttt gatcgtcgag
     5521 agaaccgaga acccgatgtg gctgtcgaac gcctacctcg tcgccgaccc cgagcgcggc
     5581 accggcgtcc tgatcgacgg caacgacgac ctcgacccgc tgctggagaa ggccgagcgc
     5641 gaggggatcg agatcaccca cgtgctcgtc acccacccgc accccgacca cgtcgccggc
     5701 ctgcgcgagg cgcgcgagaa gctcggcaac ccgccgctgg tcgcccacgc cgagaccgcc
     5761 gccgagctcg acgacgaggt cgcggtgatc ctcgccgacg gcgacaagct cgacgccaac
     5821 gggatcgagg tcgaggcgat ctacaccccc ggccacgcgg ccggccacct ggcgctgctc
     5881 gtcaacggca ccgacctgtt caccgccgac gtcctcttca aaggcacggt cgggggcacg
     5941 atggcaccgg gcgcaagcgg cttcgacgac ctccaggcct cggtgatgcg gctgatggag
     6001 ctgccgccgg agaccgtcgt ccaccccggc cacaaagagc cgacgacgat cggcgagcag
     6061 ttcgccgaga acccgttcgt gcggatctgg cgcgggctcg actcgaccgg cgaggaggaa
     6121 gtgacggtct gggaccggcc ggcgacgctg aaactgtggg cgcccgacta cgacggcggc
     6181 aacaaggcct ggatcgtctt cggcgactcc ggcgaagaag cgatcgtcgg cggcagccag
     6241 gtcgtccgcg acctctaggc gccgggacgt gaacgaggcc ccgccggagc ggggcctcgt
     6301 ggggactgcg ggagagatgg ggcgggcgct tagcggatga cggcctccat caggatccag
     6361 caaccgttgg cgtcggtggc accctggagg gcgccgcctt tcgcgagagc cggggtgaag
     6421 atcagggtgt tgtggccacc cgaggagcag cggaccatcg aggcgacgtt gccgcgcagg
     6481 gcgagctgct ggcccttctt gacgggaatg ttcacgttga acgtctcgac cttgtatttg
     6541 ccgctttcgt agttggcttc ggactggccg gtgaagctca gcttcgggcc ggtgtaggtg
     6601 accttcgcct tggtgtcgcc gagcgtggtc gaggtcgtgt gcttgaccgt cgcgatctgc
     6661 gggcggaagc tgccggcgtc ggcggcgatg atccggatcc gcttgatcgt gcccgtcttc
     6721 ggcgcgaact cgccgccgtc ggggcgaccg taggcttcgt tctggaccat cgtgcagggg
     6781 gtcagcgtgc cgggctcgca ctggaggccg ggcgagccgt tggagggctg caccgtcggg
     6841 ttcagctgcg agccgaactt gaccggcgcg gcggccatgc cggaggccgg cgcgacgatc
     6901 gcggcggcga acgcaagcag tgcgagtgcg aagccgaggc gacgggcgaa cttggtggtc
     6961 atggtggggg cggtgtgagt catcggggtt ctccttggtt ca
//
LOCUS       DASRIG010000387         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1238038, whole genome shotgun sequence.
ACCESSION   DASRIG010000387 DASRIG010000000
VERSION     DASRIG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238038"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1166)
                     /locus_tag="VFB52_10360"
     CDS             complement(216..1166)
                     /locus_tag="VFB52_10360"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZO06779.1"
                     /translation="MALPLSPPIKPQLALTRKALPLGEEWAYEPKLDGFRAIVFVDGS
                     DAYIQSRGGKELARYFPELEFAPGRWVLDGELVIRDADGMLEFDALQERIHPAQSRIE
                     LLSKEIPAEFICFDILAEGDEDLLEAPLRERRERLEALAPGANLELTPLTPDTDQAER
                     WLGSIEGVMAKDLTAKYIPGKRKGFAKVKRERTIDCVVMGWRPGKEEGTVGSLILGLY
                     DGDNLRSVGHISGFSAAAKRSMRAMLAPLETGKSGSAEPSRWTGGRDLEWVELRPELV
                     IEVGYDHAASGRIRHGARFHSFRDDKEPRECRFEQLDEGS"
     gene            complement(1191..2024)
                     /locus_tag="VFB52_10365"
     CDS             complement(1191..2024)
                     /locus_tag="VFB52_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06780.1"
                     /translation="MSAANLLEVTDLTKHFPIKRGVLIDREVDQVRAVDGVSFGVARG
                     ETLGLVGESGSGKSTACRALLQLIEPTSGSVRFEGREITGLGRRELQPLRREMQMIFQ
                     DPYGSLNPRKRVGQIVGDPLRQQGVASGRELRTRVGAMLERVGLGTEHYNRFPHEFSG
                     GQRQRIGIARALVLEPKLVVADEPVSALDVSIQAQIVNLLDDLQDELGLTYVFVAHDI
                     GVVRHIADRIAVMHEGKIVEQGSADQVCERPTDPYTKKLLAAVPVPDPRRARERRAAA
                     G"
BASE COUNT          284 a          731 c          731 g          326 t
ORIGIN      
        1 gcctacatgc gccccgagca acgcaaacgc accacccgcg ccctccgccc ctggggccga
       61 agcagccccc gctcttccgc gggtcggttc gagcagaagg tgtctagagg tgacgttgtg
      121 ccggggggtg actcgggggg aggtgccgag aacgtaaacc tcgctcgcag gcacctcccc
      181 ccgagtcagg acccgccggc ccagcccgcc ctcttctagc ttccttcgtc caactgctcg
      241 aagcggcact cccgcggctc tttgtcgtcg cggaaggagt ggaagcgggc gccgtggcgg
      301 atccggccgg aggcggcgtg gtcgtagccg acctcgatca ccagctcggg gcgcagctcg
      361 acccactcga ggtcgcggcc gccggtccag cgactcggct cggcgctgcc ggatttgccc
      421 gtctccagcg gcgccagcat cgctcgcatg ctgcgtttgg ccgcggcgct gaagccggag
      481 atgtggccga cgctgcgcag gttgtccccg tcgtagaggc cgagaatcag ggagccgacc
      541 gtgccctcct ctttgcccgg gcgccagccc atgaccacgc agtcgatcgt gcgctcgcgc
      601 ttgaccttgg cgaagccttt gcgtttgccg gggatgtatt tggcggtcag gtccttcgcc
      661 atcacgccct cgatgctgcc cagccagcgc tcggcctggt cggtgtcggg ggtcagcggg
      721 gtcagctcca ggttcgcgcc cggggccagc gcctccaggc gctcgcgccg ctcgcgcagc
      781 ggtgcctcga gcaggtcctc gtcgccctcg gccaggatgt cgaagcagat gaactcggcg
      841 gggatctcct tggagagcag ttcgatccgc gactgcgccg ggtggatccg ttcctgcagg
      901 gcgtcgaatt cgagcatccc gtcggcgtcg cggatcacca gctcgccgtc gagcacccag
      961 cggcccggcg cgaactccag ctcggggaag tagcgggcca gttctttgcc gccgcgcgac
     1021 tggatgtagg cgtcgctgcc gtcgacgaag acgatcgcgc ggaagccgtc gagcttcggc
     1081 tcgtaggccc actcctcgcc caacggcagc gctttgcggg tcagcgcaag ctgcggcttg
     1141 atcggcgggg agagcggaag ggccacgcag ggccagccta aaaggtcggg tcagccggcc
     1201 gccgcgcggc gctcgcgcgc ccgccgcggg tcgggaaccg gcaccgccgc gagcagtttc
     1261 ttcgtgtacg ggtcggtggg gcgctcgcag acctggtcgg cactgccctg ctcgacgatc
     1321 ttgccctcgt gcatcaccgc gatccggtcg gcgatgtggc ggacgacgcc gatgtcgtgg
     1381 gcgacaaaca cgtaggtcag cccgagctcg tcctggaggt cgtcgagcag gttgacgatc
     1441 tgcgcctgga tcgagacgtc gagcgccgag accggctcgt cggcgacgac cagcttcggc
     1501 tccaggacca gcgcccgggc gatcccgatc cgctgtcgct gcccgccgga gaattcgtgc
     1561 gggaagcggt tgtagtgctc ggtgccgagc ccgacccgct cgagcatcgc cccgacccgg
     1621 gtgcgcagct cgcggccgga cgcgaccccc tgctgccgca gcgggtcgcc gacgatctgg
     1681 ccgacgcgtt tgcgcgggtt gagcgagccg taggggtcct ggaagatcat ctgcatctcg
     1741 cggcgcagcg gctgcagctc gcgccgaccg agcccggtga tctcgcgccc ttcgaagcga
     1801 accgatccgg aggtcggctc gatcagttgc aggagggcgc ggcaggcggt cgacttgccg
     1861 ctgcccgact cgccgaccag gcccagcgtc tcgccgcggg cgacgccgaa gctgaccccg
     1921 tcgacggccc gcacctgatc gacctcgcgg tcgatcagca cgccgcgctt gatcgggaag
     1981 tgcttggtca ggtcggtgac ctcgagcagg ttcgcggcgc tcatggcgag ggcgcctcca
     2041 gtccgatctg accgcgaacc tcgcgcttgg cg
//
LOCUS       DASRIG010000388         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_422570, whole genome shotgun sequence.
ACCESSION   DASRIG010000388 DASRIG010000000
VERSION     DASRIG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422570"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFB52_10370"
     CDS             <1..373
                     /locus_tag="VFB52_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZO06781.1"
                     /translation="DGCTCGLGAGAGNAPTEVLTAVFEKLGIEVNADTFAMLDVADDV
                     VRPIMDRPQLVDRSSLILGYAGVYSSFLLHAERAAERFHVSTKDILVEVGKRKAVGGQ
                     EDLIIDIAAELAAAQPAPAGA"
     gene            393..935
                     /locus_tag="VFB52_10375"
     CDS             393..935
                     /locus_tag="VFB52_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06782.1"
                     /translation="MPDSRLGQIHHIAYVVDDLESAAKRLHEQFGAGPFLYLDEVPVE
                     DVSSGGEPAEFLHASAFGMCNEIPVELMVIAKMSPDRAATRFAAESTPRLQHVAYAVP
                     PAERDAIQAELEAAGMPEYLRARFGEDVVFTYHDASASLGHDIELHADSEGLRGFFAA
                     IEGAAENWDGTDLMRLPDLG"
     gene            complement(985..1647)
                     /locus_tag="VFB52_10380"
     CDS             complement(985..1647)
                     /locus_tag="VFB52_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZO06783.1"
                     /translation="MSAVPGGSEVPAAARALARAGLVDAFGHVSARGEGGVALITPAL
                     PMSEMRSEAQLVELPLGELDELPAGAPKEAWIHWAIYRARPDVGAIVRGQPPSPLAVA
                     ALADELPALLGQGAIAGAPVPVYPDSRLVREPGRAVELAAALGEADALIMRGNGALAT
                     ASTPGRALARIVLLERSADVYLRAAAAGEPRPLSTEEAAAWQAAGEELLERLWQHLRA
                     TS"
     gene            complement(1644..2627)
                     /locus_tag="VFB52_10385"
     CDS             complement(1644..2627)
                     /locus_tag="VFB52_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO06784.1"
                     /translation="MGIAHLAHAELRVTDLEASRDFFTRVLGLHASAEDDNHVYLRAW
                     QDWDHHSLVLTRAEESGLEHVAWRVDGPDAVGEFEQKLKGLGIETSWVEGGTGVNQGH
                     GDALRFFSPAGVPTELFWEVEKHVEKDPALQSKLPSHPTRYTGNGVHPRRFDHVNFLT
                     DEPQAEQEFWSEAMGVRHNYFITGDLGNGEQRLASWMAKTNLSHELAFMRNKNQNGTK
                     LHHVAYYVDTADQMTRAATILADAGVKIEWGPGQHGTSGAIFLYCWEPSGNRVEVWSG
                     GFLLFAPDWEPIRWDPETSPLGLELWGSDMPDTYLTYGTPLSTPAAAPQPA"
     gene            complement(2756..3163)
                     /locus_tag="VFB52_10390"
     CDS             complement(2756..3163)
                     /locus_tag="VFB52_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZO06785.1"
                     /translation="MTTEFINPEGVFAHPAYTGVATVTDPRKFHFFAGRTPADFETYE
                     AVHVGDFAAQYRHVMKTLDIEMKAVGCKWEDVVYRRIFTLDVDAFLASHMDDEEIASY
                     WEPGKVPPSTLIGVTRLSNPDFLIEIDLLAISE"
     gene            complement(3163..>5576)
                     /gene="ppdK"
                     /locus_tag="VFB52_10395"
     CDS             complement(3163..>5576)
                     /gene="ppdK"
                     /locus_tag="VFB52_10395"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZO06786.1"
                     /translation="GEEPGELRVQVTMALRRLEDDLDRRLGDRHDPLLVSVRSGAMHS
                     MPGMMETVLNVGLNDASVKGLVEASGDERFAWDSYRRFTQMFGNVCHGIAGERYEGLI
                     AEAKAAAGVESDVELSAADLRGLTARFKELFAAETGSEFPQDPAAQLAAAIAAVFESW
                     RGERAVHYRRINGIPDEWGTAVNVQQMVFGNKGERSCSGVAFSRDEITGAPAPSGDFL
                     PNAQGEDVVSGVRTPLDLAEMAELMPAAHAELLEILTTLERHYGDMQDTEFTVESDRL
                     YMLQTRAAKRPAQAAVRFAVDAVGEGLLDERAALATIDAGALEALLHPALDPDASFEV
                     FATGVAASPGAARGEIVFTAAAAIEAAAAGRDTILVRPFTEAEDVAGFDAARGILTSE
                     GGKASHAALVARGMGRPCVCGARALEIDLEAGTVRSNGTLLRAGETITIDGSAGLVTL
                     DEVPLREAEIGPELEQVLVWADEIRRLRVRANADTPADAVRARELGAEGIGLCRTEHM
                     FMAPERAPLMRKMVMAASEEERRAALAPLLPLQQGDFEGIFEAMAGLPVTVRLLDPPL
                     HEFLPRGGDLTPEEAERAEALAETNPMLGTRGVRLGLLHPEIYEMQARAIVRAARAVG
                     ERSGVTPVLDVMIPLVAYEQELEQIRALVERVAAEEGNEAELQVGTMIELPRACFVAD
                     RIARIADFISFGTNDLTQTALGFSRDDVEARILDRYIDSGILDRSPFETIDQPGVGWL
                     VRLAAWVGREAKPDLELGVCGEHAGDPDSIAFFDLAGVDYVSCSPYRVPIARVAAAQA
                     TNKEE"
BASE COUNT          762 a         1991 c         2001 g          822 t
ORIGIN      
        1 cgacggctgc acctgcggcc tcggcgccgg cgccggcaac gctccgaccg aggtcctgac
       61 cgcggtcttc gagaagctcg ggatcgaggt caacgccgac accttcgcga tgctcgacgt
      121 cgccgacgac gtcgtccggc cgatcatgga ccgaccccag ctcgtcgacc gctcctcgct
      181 gatcctcggc tacgcgggcg tctactccag cttcctcctc cacgccgaac gagccgccga
      241 gcgcttccac gtctccacca aggacatcct ggtggaggtc ggtaagcgca aggcggtcgg
      301 cggccaagag gacctgatca tcgacatcgc cgccgagctg gcggccgcgc aacccgcgcc
      361 cgccggcgcc tgacccctat ataaggagcg gcatgcctga ttcccgcctc ggccagatcc
      421 atcacatcgc ctacgtcgtc gacgatctcg agtcggcggc gaagcgcctg cacgagcagt
      481 tcggcgccgg ccccttcctc tacctcgacg aggtcccggt cgaggacgtc agctcgggcg
      541 gcgagcccgc cgagttcctg cacgcctcgg ccttcgggat gtgcaacgag atcccggtcg
      601 agctgatggt gatcgcgaag atgtcacccg atcgcgccgc cacgcgcttc gccgccgaga
      661 gcacgccacg cctccagcac gtcgcctacg cggtcccgcc cgccgagcgc gacgcgatcc
      721 aggccgagct ggaagccgcc gggatgcccg aatacctgcg cgcccgcttc ggcgaggacg
      781 tcgtcttcac ctaccacgac gcctccgcct cgctcggcca cgacatcgag ctgcacgccg
      841 acagcgaggg cctgcgcggc ttcttcgcgg cgatcgaagg cgctgccgag aactgggacg
      901 gcaccgacct gatgcgcctc cccgacctgg gctagaccct gtcgagtttg ttcgacccct
      961 ctgaacaaac tcgataggcc ccgctcagct cgtcgcgcgc aggtgctgcc agaggcgctc
     1021 gaggagctcc tcgccggcgg cttgccaagc ggcggcttcc tcggtgctga gcgggcgcgg
     1081 ctcgccggcg gcggcggcgc gcaggtagac gtcggcgctg cgctcgagca ggacgatgcg
     1141 ggcgagggcg cggccggggg tggaggcggt ggcgagggcg ccgttgccgc gcatgatcag
     1201 cgcgtcggcc tcgcccaggg ccgcggccag ctcgacggcg cggccgggct cgcggaccag
     1261 gcgggagtcg gggtagaccg gcaccggggc gccggcgatc gcgccctggc cgagcagcgc
     1321 cggcagctcg tcggcgagcg cggcgacggc caggggggag gggggctggc cgcggacgat
     1381 cgcgccgacg tcggggcggg cgcggtagat cgcccagtgg atccaggcct ctttcggcgc
     1441 gccggccggc agctcgtcga gctcacccag cggcagctcg accagctgcg cctcggagcg
     1501 catctcggac atcggcagcg ccggggtgat cagcgccacc ccgccctcgc cccgcgcgga
     1561 gacgtggccg aaggcgtcga ccaggccggc gcgcgcgagc gcccgggcgg cggcgggtac
     1621 ctcgctgccg cccgggaccg cgctcatgcc ggctgcggtg cagccgcggg ggtggacagg
     1681 ggggtgccgt acgtcaggta ggtatccggc atgtccgagc cccacaactc gagtcccagc
     1741 ggggaggtct ccgggtccca gcggatcggc tcccagtccg gcgcgaacag caggaagccg
     1801 ccgctccaga cctcgacgcg gttgcccgag ggctcccagc agtagaggaa gatcgcgccg
     1861 ctggtcccgt gctgccctgg cccccactcg atcttcacgc cggcatcggc gaggatcgtc
     1921 gccgcccgcg tcatctggtc ggcggtgtcg acgtagtagg cgacgtggtg cagcttggtg
     1981 ccgttctggt ttttgttgcg catgaaggcc agctcatgcg agaggttcgt cttcgccatc
     2041 caggaggcga gccgctgctc gccgttgccg aggtcgccgg tgatgaagta gttgtggcgg
     2101 acccccatcg cctcgctcca gaactcctgc tcggcctggg gttcgtcggt gaggaagttg
     2161 acgtggtcga agcggcgcgg gtgcacgccg ttgccggtgt agcgggtcgg gtggctgggc
     2221 agcttcgact gcagcgccgg gtccttctcg acgtgtttct cgacctccca gaaaagctcg
     2281 gtcgggacgc cggccgggga gaagaagcgg agcgcgtcgc cgtggccctg gttgaccccg
     2341 gtgccgccct cgacccaact ggtctcgatc cccagcccct tcagcttctg ctcgaactcg
     2401 ccgaccgcgt cggggccgtc gacccgccag gcgacgtgct cgaggccgct ctcctcggcc
     2461 cgggtgagga ccagcgagtg gtggtcccag tcctgccagg cgcgcaggta gacgtggttg
     2521 tcgtcctcgg cgctggcgtg cagccccagg accctggtga agaagtctcg cgaagcctcg
     2581 aggtcggtga cccggagctc ggcatgtgcc aagtgggcga ttcccatctc tgtctctccc
     2641 atccctcggt ggagtgtcga gcgtaattga ttacgcagtg cggaacaacg tccttaacta
     2701 cgaatcatta tagggaatta tggcgcgacg acaaaggtgc cgccgcgccg cgccgctatt
     2761 cgctgatcgc gagcagatcg atctcgatca ggaagtcggg gttggagagg cgggtgaccc
     2821 cgatcagggt gctcggcggc accttgcccg gctcccagta ggaggcgatc tcctcgtcgt
     2881 ccatgtggga cgcgaggaag gcgtcgacgt cgagggtgaa gatccgccgg tagacgacgt
     2941 cctcccactt acagccgacc gctttcatct cgatgtcgag cgttttcatc acgtgccggt
     3001 actgggcggc gaagtcgccg acgtggacgg cctcgtaggt ctcgaagtcg gccggggtgc
     3061 ggccggcgaa gaagtggaac ttgcgcgggt cggtgacggt ggcgacaccg gtgtaagccg
     3121 gatgggcgaa gacgccctcg gggttgatga actccgtggt cattactcct ccttgttggt
     3181 ggcctgtgcc gccgcaaccc gggcgatcgg cacgcggtac ggcgagcagc tgacgtagtc
     3241 gacgccggcg aggtcgaaga aggcgatcga gtcggggtcg ccggcgtgct cgccgcagac
     3301 gcccagctcc aggtccggct tggcctcgcg cccgacccag gcggcgagcc ggaccagcca
     3361 gccgaccccg ggctggtcga tcgtctcgaa cgggctgcgg tcgaggatgc cgctgtcgat
     3421 gtagcggtcg aggatgcgcg cctcgacgtc gtcgcggctg aacccgagcg cggtctgggt
     3481 gaggtcgttg gtgccgaagc tgatgaagtc ggcgatccgg gcgatccggt cggcgacgaa
     3541 gcaggcgcgc ggcagctcga tcatcgtccc gacctgaagc tcggcctcgt tgccctcctc
     3601 ggcggcgacc cgctcgacca gcgcccggat ctgctcgagc tcctgctcgt aggcgaccag
     3661 cgggatcatc acgtcgagca ccggggtcac cccggagcgc tcgccgaccg cgcgcgcggc
     3721 gcgcacgatc gcccgcgcct gcatctcgta gatctccggg tgcaggaggc cgaggcggac
     3781 gccgcgggtg ccgagcatcg ggttggtctc ggccagcgcc tcggcccgct cggcttcctc
     3841 gggggtgagg tcgccgcccc gggggaggaa ctcgtgcagc ggcgggtcga gcaggcgcac
     3901 ggtcaccggc agcccggcca tcgcctcgaa gatcccttcg aagtcgccct gctgcagcgg
     3961 cagcagcggc gccagcgccg cgcgccgctc ctcctcgctc gccgccatca ccatcttgcg
     4021 catcagcggc gcccgctccg gcgccatgaa catgtgctcg gtgcggcaga ggccgatccc
     4081 ttcggcgccg agctcacgcg cccggacagc gtcggccggg gtgtccgcgt tggcgcgcac
     4141 gcgcagccgc cggatctcgt cggcccacac caggacctgc tccagctcgg gcccgatctc
     4201 ggcctcgcgc agcggtacct cgtcgagggt caccagcccg gccgagccgt cgatcgtgat
     4261 tgtctcgccg gcccgcagca gggtcccgtt cgagcgcacc gtgcccgcct cgaggtcgat
     4321 ctccagcgcc cgcgccccgc agacgcaggg ccggcccatg ccgcgggcga ccagggcggc
     4381 gtgggaggcc ttgccgccct cggaggtgag gatgccgcgg gcggcgtcga agccggcgac
     4441 gtcctcggcc tcggtgaagg ggcggacgag gatcgtgtcg cggcccgcgg ccgccgcctc
     4501 gatcgcggcg gcggcggtga agacgatctc cccgcgcgcc gcccccggcg aggcggcgac
     4561 gccggtggcg aagacctcga aggaggcatc ggggtcgagg gcggggtgca gcagcgcctc
     4621 cagggcgcct gcgtcgatcg tcgccagcgc cgcgcgctcg tccagcaacc cctcgccgac
     4681 cgcgtcgacc gcgaaccgca ccgccgcctg cgcgggccgc ttcgccgccc gcgtctgcag
     4741 catgtagagg cggtcggatt cgaccgtgaa ctcggtgtcc tgcatgtcgc cgtagtggcg
     4801 ctccagggtg gtgaggatct ccagcagctc ggcgtgcgcg gccggcatca gctccgccat
     4861 ctcggccagg tcgagcggcg tgcgcacccc ggagaccacg tcctcgccct gggcgttggg
     4921 gaggaagtcg ccgctgggcg cgggcgcgcc ggtgatctcg tcgcggctga acgcgacgcc
     4981 cgagcacgag cgctcgccct tgttgccgaa gaccatctgc tgcacgttga ccgcggtgcc
     5041 ccactcgtcg gggatgccgt tgatccggcg gtagtggacg gcgcgctcac cgcgccagga
     5101 ctcgaacacg gcggcgatcg cggcggccag ctgcgcagcc gggtcctggg ggaactcgct
     5161 cccggtctcg gccgcgaaca gctccttgaa gcgcgcggtc agcccgcgca ggtcggcggc
     5221 cgagagctct acgtcggact cgaccccggc cgccgccttg gcctcggcga tcagcccctc
     5281 gtagcgctcg ccggcgatcc cgtggcagac gttgccgaac atctgggtga agcgccggta
     5341 ggagtcccag gcgaagcgct cgtcgccgct ggcctcgacc aggcctttga cgctcgcgtc
     5401 gttgagcccg acgttgagca ccgtctccat catcccgggc atcgagtgca tggcgccgga
     5461 ccgcacgctc accagcagcg ggtcgtgccg gtcgccgagg cggcggtcga gatcgtcctc
     5521 cagccggcgg agcgccatcg tgacctgcac ccgcagttcg cccggctcct cgccgg
//
LOCUS       DASRIG010000389         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_720041, whole genome shotgun sequence.
ACCESSION   DASRIG010000389 DASRIG010000000
VERSION     DASRIG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720041"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFB52_10400"
     CDS             <1..1186
                     /locus_tag="VFB52_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06787.1"
                     /translation="DAGGRMLSYEDLIAANAPLARPVERAPEETAWLFYTSGTTGRPK
                     GAMLSHRNLRQMILSHLADVRSFEPGEPVLHAAPLSHGSGCVLLSCIARGARNVIFSH
                     RSFDGDKVLETVRDEGIRSISFLAPTQIVVLNRAAAAAAENDGVLAHSLESVCYGGAP
                     MFAEDLRAAMALFGPVFVQIYGQGEAPMTISVLAISDHVRFEAGGDNRITSAGIPRTD
                     VEIRVIDEGGAELPRGERGEVIVRGDVVMAGYWDNPEATSAALSDGWLRTGDIGLVDD
                     TGYLHLLDRSKDMIISGGNNIYPREVEEALLLMPEVQATAVIGVPDPYWGESVHALVV
                     TRDGDSLTEEAVIEHCRTMLSSYKKPRTVEFLSELPTNAYGKVLKRELRAERSVGETR
                     KA"
     gene            1305..2429
                     /locus_tag="VFB52_10405"
     CDS             1305..2429
                     /locus_tag="VFB52_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZO06788.1"
                     /translation="MKPIGDQSRDGRNELWRRGRALLLVLAAVVLACVVVAGCGSDSS
                     SDSSGSESGSGTTASDENSGASGDPVSIAMFLVATANTHQQAALKGAEQVVEEDGNAS
                     LRAFNGNFDPATQSNQIEDATASGQYDAFLVNSIDGTQTIPAITKALDEEIVVVCGFS
                     ICGPNQDEFAKELPVTAQVASDYFAVGKAEADALVKGCEEKGFDPCNVVLFNGASTLA
                     AGIAITESIKEVVAEHPNINIVAEAEGLFLSDPSYKAMKPIIQAHPDLNAVIAAGDQE
                     ISGALQAIEESPLKGKDILLIGDGASEIGAKGVESGVWYASSILRPFNEGKVEAEIAI
                     AAARGEEPAEDLINSAEDPDFPTGYIDASNVDKWEPEWAG"
     gene            2475..>3639
                     /locus_tag="VFB52_10410"
     CDS             2475..>3639
                     /locus_tag="VFB52_10410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO06789.1"
                     /translation="MSQSPPLVEARSLSKRFGGAQALKDVDLAVVAGEVHGLVGENGA
                     GKSTLGKCVAGVLHPDGGQLLVDGAPVSYKVPRDALADGITIVEQELALLPAMSVVDN
                     VLLGSSAAGEARSRRARRTAVQELSDRFGLGLEVGKTVERLSVAEQQKVEILRALARR
                     ARLIVMDEPTARLARDEADNLLAVIRRLSEDGTAIVLVSHFLDDVLSVADRVTVMRNG
                     EVVKNGEAAGETPASLVAAMLGRDASLEFPDKRPPRRDSAPVLAVRDLADGQRVDGVS
                     FEVRRGEIVGLGGLVGSGRSEVARLIFGAERPQRGTIEIDGEPTRIRSVRRAVRAGIS
                     YLPESRKDLGLFLDLSNQENTTLAHLGEVTRGGLIVSGKERHDTAEILERLGVN"
BASE COUNT          618 a         1257 c         1235 g          529 t
ORIGIN      
        1 cgacgccggt ggccggatgc tctcctacga ggatctgatc gccgccaacg cgccgctcgc
       61 gcgtccggtc gagcgggccc ccgaggagac cgcctggttg ttctacacct cgggcacgac
      121 cggacgcccg aaaggggcga tgctgagcca tcgcaacctg cgccagatga tcctcagcca
      181 cctcgccgac gtgcgctcct tcgagcccgg cgagccggtc ctgcacgccg cgccgctgag
      241 ccacggatcg ggatgcgtgc tgctctcctg catcgcccgc ggcgcccgca acgtgatctt
      301 ctcccaccgt tccttcgacg gcgacaaggt cctggaaacc gttcgcgacg agggaatccg
      361 gtcgatctcc ttcctcgccc cgacccagat agtcgtgctc aaccgggccg ccgccgcggc
      421 cgccgagaac gacggcgtcc tcgcccactc tctggagtcg gtctgttatg gcggcgcgcc
      481 gatgttcgcc gaggacctgc gcgcggcgat ggccctgttc gggccggtgt tcgtccagat
      541 ctacggccag ggggaggcgc cgatgacgat ctcggtgctg gcgatctccg accacgtgcg
      601 cttcgaggcg ggcggcgaca atcggatcac ctcggctggg atcccccgca ccgacgtcga
      661 gattcgcgtc atcgacgagg ggggtgcgga gctcccgcgc ggcgagcggg gcgaagtcat
      721 cgtccgcggc gacgtcgtga tggccggcta ctgggacaac cccgaggcaa ccagcgcggc
      781 gctcagcgac ggctggttgc ggaccggcga catcggcctg gtcgacgaca ccggttacct
      841 ccatctgctc gaccgctcca aggacatgat catctctggc ggcaacaaca tctacccccg
      901 cgaggtcgag gaggcgctgc tgctgatgcc ggaggtccag gcgaccgcgg tgatcggcgt
      961 tcccgaccct tactgggggg agagcgtgca cgctctggtc gtcacccgcg acggtgacag
     1021 cctgaccgag gaggcggtga tcgagcactg ccggaccatg ctcagcagct acaagaagcc
     1081 gcgcaccgtc gagttcctct ccgagctgcc gaccaatgcg tacggcaagg tgctcaagcg
     1141 cgagctgcgg gccgagcgta gtgtcggtga gacgagaaaa gcgtgactat tcgtcccgcc
     1201 cgaccgtgga ttcacctcac tgtacgaacc tagcatgaca ctgtcggtga acccgacgta
     1261 ttaaccgtgt caacggcaat gggtgcctag tgaggagaga tttgatgaag cctatcggtg
     1321 atcagtcacg cgacggtcgc aacgagctgt ggcggcgcgg ccgggcgttg ctgctcgtgc
     1381 tggcggcggt tgtgctcgca tgcgtcgtgg tagcggggtg cggctcggac tcgagctcgg
     1441 actccagtgg gtccgaaagc ggctcgggaa cgaccgcgag cgacgaaaac tcgggcgcct
     1501 ccggcgatcc cgtgagcatc gcgatgttcc tggtggcgac ggcgaacacc caccagcagg
     1561 cggccctcaa aggcgccgaa caggtcgtcg aggaagacgg caacgcctcg ctgcgggcct
     1621 tcaacggaaa cttcgacccg gcgacccagt ccaaccagat cgaagacgcg accgcctccg
     1681 ggcagtacga cgccttcctg gtcaactcga tcgacggcac gcagaccatc cccgcgatca
     1741 ccaaggcgct cgacgaagaa atcgtcgtcg tctgcggctt ctcgatctgc ggtcccaacc
     1801 aggacgaatt cgccaaagag ctgccggtga ccgcccaggt cgcctccgac tacttcgcgg
     1861 tcggcaaagc cgaggccgac gccttggtca agggctgcga ggagaaaggg tttgacccct
     1921 gcaacgtcgt cctcttcaac ggcgcttcga cgctggcggc cgggatcgcg atcaccgaaa
     1981 gcatcaagga agtggtcgcc gaacacccga acatcaacat cgtcgccgaa gccgaagggc
     2041 tcttcctctc cgacccgtcc tacaaagcga tgaagccgat catccaggcc cacccggacc
     2101 tgaacgcggt gatcgcggcc ggcgaccagg aaatcagcgg cgccctgcag gcgatcgaag
     2161 agtcgccgct gaaaggcaaa gacatcctcc tgatcggcga cggagcgagc gaaatcgggg
     2221 ccaaaggcgt cgaatccggc gtctggtacg cgagctcgat cctgcgtccc ttcaacgagg
     2281 gcaaagtcga ggccgaaatc gcgattgccg ccgcacgcgg ggaagaaccg gccgaggacc
     2341 tgatcaactc ggccgaagac cccgacttcc cgaccgggta catcgacgcg tccaacgtcg
     2401 acaaatggga accggaatgg gccggctgag cggctcccgg gaccgctgag cgaccactac
     2461 caccgctgaa cccgatgagc cagagccccc cactggtcga ggcgcgcagc ctcagcaagc
     2521 ggttcggcgg cgcccaggcc ctgaaggatg tggacctcgc cgttgtggcc ggcgaggtcc
     2581 acggcctggt cggcgagaac ggcgccggca agtcgacgct cggcaagtgc gtggccggcg
     2641 tcctgcaccc cgacggcggc cagctgctgg tcgacggggc cccggtcagc tacaaggttc
     2701 cgcgcgacgc gctcgctgac gggatcacga tcgtcgagca ggagctggcg ctgctgccgg
     2761 cgatgtcggt tgtcgacaac gtcctgctcg gcagctccgc cgcgggcgag gcgcgttcgc
     2821 gcagggcgcg ccgcaccgcg gtccaggagc tgagcgaccg cttcgggctc gggctcgagg
     2881 tcgggaagac ggtcgagcgg ctctcggtcg ccgagcagca gaaggtcgag atcctccgcg
     2941 cgctggcccg ccgggcgcgg ctgatcgtga tggacgagcc gaccgcccgc ctcgcccgtg
     3001 acgaggccga caacctgctg gcggtgatcc ggcgcctctc ggaggatggc accgcgatcg
     3061 tcctcgtctc ccacttcctc gacgacgtcc tctcggtcgc cgaccgggtc acggtgatgc
     3121 gcaacggcga ggtggtgaag aacggcgagg cggccggcga gacgcctgcc agcctggtcg
     3181 cggcgatgct ggggcgcgac gcctcgctcg agttcccgga caaacggccg ccccgccgtg
     3241 actccgcccc ggtcctcgcg gtgcgcgacc tggccgacgg ccagcgcgtc gacggggtca
     3301 gcttcgaggt ccggcgcggc gaaatcgtcg gcctcggcgg gctggtcggc agcggccgct
     3361 cggaggtcgc gcggctgatc ttcggcgccg agcgcccgca gcgcggcacg atcgagatcg
     3421 acggcgagcc gacccggatc cgctcggtgc gccgcgccgt ccgcgcgggc atctcctacc
     3481 tgcccgagag tcgcaaggac cttggcctct tcctcgacct ctccaaccag gagaacacga
     3541 cgcttgccca cctcggcgag gtcacccggg gtgggctgat cgtgtccggc aaggagcgcc
     3601 acgacacggc cgagattctc gagcggctcg gcgtcaacc
//
LOCUS       DASRIG010000390         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1745896, whole genome shotgun sequence.
ACCESSION   DASRIG010000390 DASRIG010000000
VERSION     DASRIG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745896"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFB52_10415"
     CDS             complement(<1..405)
                     /locus_tag="VFB52_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO06790.1"
                     /translation="MSEFFNQYFDFTVMGDHFGEVLDGFFKNLLLFAVVAVLALSWGL
                     ILALLRQLPGKKLMPLRGVTIAYIDCLRAIPILGVILLISGSLPYLEFVPKDIRIPHF
                     LGQTDPFWYGALALTLVYGAYMAEVYRAGIEAI"
     gene            complement(498..1328)
                     /locus_tag="VFB52_10420"
     CDS             complement(498..1328)
                     /locus_tag="VFB52_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZO06791.1"
                     /translation="MNKTRLSALFALLLVAVVAMVAAGCGSDDDSGSTGGAETSASTE
                     GGGGGGEPLSVGSDIPYPPFEQGKAGEYTGFDIELMEAIGEKIGRTPEFTDTSFETIF
                     RDVAQGKFDAAISAATITEEREKTVAFSDPYYLSEQAILVEEGSEITGLADLAGKTVG
                     AQQGTTGLELGKKEANAGDLRPYPEGPEAVNALKSGVIEAVIIDAPVAQNAVEEAGGV
                     EIAEKVPTEEEYGIALAKDNTALLEEINQGLAEVIEDGTYTTIYEKWFKLEPPKEIVG
                     "
     gene            1509..2474
                     /locus_tag="VFB52_10425"
     CDS             1509..2474
                     /locus_tag="VFB52_10425"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZO06792.1"
                     /translation="MAQDQLLNRPCGGRFGNVVESIGNTPLVELPRFSPKPDVRIYAK
                     LEGHNPTGSVKDRVAKSMIEAAEAEGAIEPGQTILEPTSGNTGIALAMICRRKGYPLK
                     VVMPDNVTEERTQLLKMYGAEIVYSEGAKGSNGAVEVALELAEQPGFYMPYQYGNENN
                     PLAHYNGTAVEILDELDEVAAFVGGLGTGGTLMGNGRRLKEANPETVIVAAEPMQGEL
                     VDGLRSLDDGFIPPIIDLSILDRKIMVSNRDAIVWTKKLLEEEGVFAGVSAGAIAAIA
                     SRIANELDGGNVVFLIPDDGWKYLSSGVYTKPIEEIENLDSTVWW"
     gene            2468..2896
                     /locus_tag="VFB52_10430"
     CDS             2468..2896
                     /locus_tag="VFB52_10430"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZO06793.1"
                     /translation="MVAAPARHTPMRIAQQLIDEMVAHSREDLPNECCGMLGGKDGEA
                     TTLIRVANAAASPLRYEMDPQAQYNALKQIEDGGEELLAIYHSHTKSAAYPSQTDVNQ
                     AVAWPDQVYVIVSLADADAPDVKAFLLKDLKIADVELDVT"
     gene            2874..3347
                     /locus_tag="VFB52_10435"
     CDS             2874..3347
                     /locus_tag="VFB52_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06794.1"
                     /translation="MSSSTSHKRPGSHSRGAHGGGGSDGGHEGGGGGGGGGGGKLVKV
                     AYGRNQSEAELMQGLLSEEGIPSVLKRSGGFDNPDFLAAGPHDVLVSEQIAPRAREVL
                     GDLVLEGETEEQRELEAFGMTARSAGPSTGQFALWFGIVVIGAVILIWVLYQVTG"
     gene            3347..3613
                     /locus_tag="VFB52_10440"
     CDS             3347..3613
                     /locus_tag="VFB52_10440"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZO06795.1"
                     /translation="MSDPRFVENVELVKVALVPNPVEAEMVRGLLESEGIPVLLEEAG
                     TSPGAKNGPRLIFGGPQRVMVHAHRADEARALLEAQPEEQTSEP"
     gene            complement(3615..>4063)
                     /locus_tag="VFB52_10445"
     CDS             complement(3615..>4063)
                     /locus_tag="VFB52_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06796.1"
                     /translation="DDSLEALRGTAERLYAQLVMAANNSALPPAWTPGRFYRYLRWAW
                     LVEGGAQYFARQVGLYRAAVLRRLREGSRPAFPPSPRDAVILGGTVFDLLENERGPQA
                     CERLVDGLLPGGPAVTLEDAFDARFRDIEDAWRDYLRVMVKPAGVG"
BASE COUNT          699 a         1290 c         1397 g          677 t
ORIGIN      
        1 gatcgcttcg atccccgccc tgtagacctc ggccatgtag gcgccgtaga cgagcgtcag
       61 ggccagggcg ccgtaccaga agggatccgt ttggccgaga aaatgaggga tgcggatgtc
      121 tttggggacg aactccagat aggggaggct gccggagatc agcaggatca cgccgaggat
      181 cggaatcgcc cgcaagcagt cgatgtaggc gatcgtgacc ccacgcagcg gcatgagctt
      241 tttcccgggc aactgccgga gcagggcgag gatcaggccc caggagagcg cgagcacggc
      301 taccacggcg aagagaagca gatttttgaa gaagccgtcc aggacttcgc cgaagtggtc
      361 tcccatcacg gtgaagtcga agtactggtt gaagaattcg ctcatcgctg tctcctcgtt
      421 gagcccggcg ggaaatgcag gaggggcgcc atcccaggcg cccctcccgt tgcaatcaga
      481 ctacgcgtct ccggccgcta gccgacgatc tctttcggcg gttcgagttt gaaccacttt
      541 tcgtagatcg tcgtgtaggt gccgtcttcg atcacttccg cgaggccctg gttgatttct
      601 tcgagcagcg cggtgttgtc tttggcgagg gcgattccat actcttcttc ggtcgggact
      661 ttttcggcga tttcgacgcc acccgcttct tcgaccgcgt tttgcgcgac cggggcgtcg
      721 atgatcaccg cttcgatgac tcccgatttg agcgcgttca ccgcttctgg tccttccggg
      781 taggggcgaa gatcaccggc atttgcttct ttcttgccga gttcgaggcc ggtcgtgccc
      841 tgctgggcac ccaccgtttt cccggcgagg tcggcgaggc cggtgatttc actgccttcc
      901 tcgaccagga tcgcctgttc ggagaggtag taggggtcgg agaaggcgac cgtcttttcg
      961 cgttcttcgg tgatcgtcgc cgccgagatc gccgcatcga acttgccctg ggcgacgtcg
     1021 cggaagatcg tttcgaacga ggtgtcggtg aattcgggcg tgcggccgat cttttcgccg
     1081 atcgcttcca tcagttcgat gtcgaagccg gtgtattcac ccgctttgcc ctgctcgaag
     1141 ggcgggtacg ggatgtcgga gccgaccgac agcggttcgc cgccgcctcc accgccttcg
     1201 gtgctggcgc tggtttcggc accgccggtg ctgccggagt cgtcgtcgga gccgcatccc
     1261 gctgcgacca tcgcgaccac tgctacgagg agaagcgcga acaacgcgct cagccgggtc
     1321 ttgttcatcg atcctgcctt tcgatccagg gttgaatcaa ccgtgtaacg gggatgtagc
     1381 ccgattgggc gaaaatacac attcgttccg ccggttggta ttgaaggttg gtctgccaga
     1441 ccaaatcgaa cccgctttgc cgggcctcca tccctcgcta cactttctga agtaaaggga
     1501 acccggcgat ggctcaggac caacttctca accgaccctg cggggggcgc ttcggcaatg
     1561 tcgtcgagtc gatcggcaat accccgctgg tcgagctgcc gcgcttctcg ccgaagcccg
     1621 acgtgcggat ctacgcgaag ctcgagggcc acaacccgac cggctcggtc aaggaccgcg
     1681 tcgccaaatc gatgatcgag gcggctgagg ccgaaggggc gatcgaaccg gggcagacga
     1741 tcctcgaacc gacgtccggc aacaccggga tcgcgctggc gatgatctgc cggcgcaagg
     1801 gctacccgct gaaggtcgtg atgccggaca acgtgaccga ggagcgcacc cagctgctga
     1861 agatgtacgg ggcggagatc gtctactcgg agggcgccaa gggctccaac ggcgcggtcg
     1921 aggtggcgct ggagctggcc gagcagcccg gcttttacat gccctaccag tacggcaacg
     1981 agaacaaccc gctcgcccac tacaacggga ccgcggtcga gatcctcgac gagctcgacg
     2041 aggtcgccgc ctttgtcggc gggctcggca ccggtggcac cctgatgggc aacggccgcc
     2101 ggctcaagga ggccaacccg gagacggtga tcgtcgccgc cgagccgatg cagggcgagc
     2161 tcgtcgacgg cctgcgctcg ctcgacgacg gcttcatccc gccgatcatc gacctctcga
     2221 tcctcgatcg caaaatcatg gtctccaacc gcgacgcgat cgtctggacg aagaaactgc
     2281 tcgaggagga gggcgtcttc gccggcgtct cggccggcgc gatcgccgcg atcgcctccc
     2341 gcatcgccaa tgagctcgac ggcggcaacg tcgtcttcct gattcccgac gacggctgga
     2401 agtacctctc ctccggcgtc tacaccaagc cgatcgagga gatcgaaaac ctcgattcca
     2461 ctgtctggtg gtagcggcgc ccgctcgcca cacgccgatg cggatcgcgc agcagctgat
     2521 cgacgagatg gtcgcgcact cgcgcgagga tctgccgaac gagtgctgcg ggatgctcgg
     2581 tggcaaggac ggcgaggcga cgaccctgat ccgcgtcgcc aacgcggccg ccagcccgct
     2641 gcgctacgag atggacccgc aggcgcagta caacgctttg aagcagatcg aagacggcgg
     2701 cgaggagctg ttggcgatct accactcgca caccaagagc gcggcgtatc cgtcgcagac
     2761 cgatgtcaac caggcggtcg cctggcccga ccaggtctac gtgatcgtct cgctcgccga
     2821 cgccgacgcg cccgacgtca aggccttcct gctgaaggac ctgaagatcg ccgatgtcga
     2881 gctcgacgtc acataagcga cccgggtccc acagtcgcgg cgcccacggg ggcggcgggt
     2941 ccgacggcgg ccatgagggc ggcggcggag gcggaggagg cggtggcggc aagctggtca
     3001 aggtcgccta cggtcgcaac cagtcggaag ccgaactgat gcaggggctg ctgagcgagg
     3061 aggggatccc gagcgtgctg aagcgcagcg gcggcttcga caaccccgac ttcctcgccg
     3121 ccggaccgca cgacgtgctg gtcagcgagc agatcgcgcc gcgggcgcgg gaagtgctcg
     3181 gcgacctggt gctggagggc gagaccgaag agcagcggga gctggaagcg ttcgggatga
     3241 ccgcgcgcag cgccggtccc tcgaccgggc agttcgcgct ctggttcggc atcgtcgtga
     3301 tcggcgccgt gatcctgatc tgggtcctct accaggtgac cggctgatga gcgacccgcg
     3361 cttcgtcgag aacgtcgagc tggtcaaagt cgccttggtc ccgaatccgg tcgaggcgga
     3421 aatggtccgc ggcttgctcg aaagcgaggg gatcccggtg ctcctggagg aagccggtac
     3481 gagtcccggc gcgaagaacg ggccgcggct gatcttcggc ggtccgcagc gggtgatggt
     3541 gcacgctcac cgggccgatg aagcgcgcgc cctgctcgag gcgcagcccg aagagcagac
     3601 gagcgaaccc tgagtcagcc gacgccggcg ggtttcacca tcacccgcag gtagtcccgc
     3661 caggcgtcct cgatgtcgcg gaagcgggcg tcgaaggcgt cttcgagcgt gaccgcgggc
     3721 ccgcccggga gcaggccgtc gacgaggcgc tcgcaggcct gggggccgcg ttcgttctcg
     3781 agcaggtcga agacggtgcc gccgaggatc accgcgtcgc gcggggaggg cgggaaggcg
     3841 ggacgggagc cctcgcgcag gcggcgcagg accgcggccc ggtagaggcc gacctggcgg
     3901 gcgaagtact gggcgccgcc ttcgaccagc caggcccagc gcaggtagcg gtagaagcgg
     3961 ccgggggtcc aggccggcgg cagggcgctg ttgttggcgg ccatcaccag ctgggcgtag
     4021 aggcgctcgg cggtgccgcg cagcgcttcc agcgagtcgt cac
//
LOCUS       DASRIG010000391         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_197247, whole genome shotgun sequence.
ACCESSION   DASRIG010000391 DASRIG010000000
VERSION     DASRIG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197247"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFB52_10450"
     CDS             complement(<1..931)
                     /locus_tag="VFB52_10450"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZO06797.1"
                     /translation="MSTPVKVTVTGAAGQIGYALLFRIASGQMLGPDTPVELSLLEIP
                     DAIKAAEGTAMELDDCAFPLLHKIDITDDAKQAFDGTNVGLLVGARPRGPGMERSDLL
                     EANGGIFKPQGEAINAGAADDVRVLVVGNPANTNALIAMSNAPDVPRERFTAMMRLDH
                     NRAIAQVANKLGKQVSAVTNMTAWGNHSPTQYPDLVNAKVDGASAWDAVDDEAWIRDE
                     YIPRVGKRGAEIIEARGSSSAASAANAAIDHVRDWVLGTPDGDWVSMAVPADGSYGIG
                     EGIICGLPCTCSGGEWSVVQGLDVPDFSRERIDA"
     gene            complement(970..2184)
                     /locus_tag="VFB52_10455"
     CDS             complement(970..2184)
                     /locus_tag="VFB52_10455"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZO06798.1"
                     /translation="MAKIKVQNPVVELDGDEMTRIIWSFIKEQLILPYLDVELEYYDL
                     GIESRDASDDQITVDAANAIKKHGVGVKCATITPDEARVEEFGLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNIPRLVPGWTKPIVIGRHAFGDQYRATDLVVPGEGQLTLTYTPKD
                     GSEPIELDVYDFPSGGIAMAMYNLDDSIRDFARASMNYGLNRNYPVYLSTKNTIMKRY
                     DGRFKDLFEEIYENEFKADFEAAGITYEHRLIDDMVAAALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMSADGKTVEAEAAHGTVTRHYRQHQKGEPTSTNPIASIFA
                     WTRGLSARGRMDETPEVSAFAETLERVCIETVESGKMTKDLALLVGADQEYQTTQEFL
                     ASIDENLQAAMA"
     gene            2265..>2702
                     /locus_tag="VFB52_10460"
     CDS             2265..>2702
                     /locus_tag="VFB52_10460"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HZO06799.1"
                     /translation="MATRERQTFGNHEVANQPPPLSGYNVFEADTPLREAVRREGAEW
                     AEPRIAAAGAYAGSAHAQELGRLANDNGPKLKTHDRYGNRVDEVEFHPAWHELLGTAV
                     EHELHSAPWKDPRPGAHVARGAAFMCMSQAEAGIGCPISMTYSV"
BASE COUNT          410 a          882 c          924 g          486 t
ORIGIN      
        1 tggcgtcgat ccgctcgcgt gagaagtcgg gcacgtcgag gccctggacg acgctccact
       61 cgccgccgct gcaggtgcag ggcaggccac agatgatgcc ctcgccgatc ccgtaagaac
      121 cgtcggcggg aaccgccatc gagacccagt cgccgtcggg cgtgccgagc acccagtcgc
      181 gcacgtgatc gatcgccgcg ttggcggcgg aggcggcgct ggaggagccg cgagcctcga
      241 tgatctcggc gccgcgcttg ccgacccgcg ggatgtactc gtcgcggatc caggcttcgt
      301 cgtcgaccgc gtcccaggcg ctggcgccat cgaccttggc gttgaccagg tccgggtact
      361 gggtcggcga gtgattgccc caggcggtca tgttggtgac cgcggagacc tgcttgccga
      421 gcttgttggc aacctgggcg atcgcccggt tgtggtcgag ccgcatcatc gcggtgaagc
      481 gctcacgcgg gacgtcgggg gcgttcgaca tcgcgatcag ggcgttggtg ttggccgggt
      541 tcccgaccac cagcaccctg acgtcgtcgg ccgcgccggc gttgatcgct tcgccttgcg
      601 gcttgaagat gccgccgttg gcctcgagca gatcggaacg ctccatgccc gggccgcgcg
      661 ggcgggcacc gaccaggaga ccgacgttgg tgccgtcgaa ggcctgcttg gcatcgtcgg
      721 tgatgtcgat cttgtgcagc agcgggaagg cgcagtcgtc gagctccatc gcggtgccct
      781 cggcggcctt gatcgcgtcc ggaatctcca acaggctcag ctcgaccggc gtgtcggggc
      841 cgagcatctg gccgctggcg atacggaaca gcagcgcgta gccgatctgg ccggcagcgc
      901 ccgtgacggt gaccttgacg ggggtgctca aagaaatcct ctcctcggaa tcgcggtttg
      961 ctcgcgccgc taggccattg cggcttgcag gttctcgtcg attgaggcca ggaactcctg
     1021 cgtggtctgg tactcctggt ctgcgccgac gagcagcgcg aggtctttgg tcatcttgcc
     1081 gctctcgacc gtctcgatgc agacccgctc gagggtctcg gcgaacgcgg agacctcagg
     1141 ggtctcgtcc atccgcccgc gggcggagag cccacgcgtc caagcgaaga tcgaagcgat
     1201 cgggttggtg gaagtcggtt cgcctttttg atgctggcgg tagtgacggg tgaccgtgcc
     1261 gtgcgcggcc tcggcctcga ccgtcttacc gtcggcgctc atcagcacgc tggtcatcag
     1321 cccgagtgag ccgaagccct gggccacggt gtcggactgg acgtcgccgt cgtagttctt
     1381 gcaggcccac acgtagccgc cttcccactt cagcgccgcc gcaaccatgt cgtcgatcag
     1441 gcggtgttcg taggtgatcc cggcggcctc gaagtcagct ttgaactcgt tctcgtagat
     1501 ctcttcgaag aggtccttga agcggccgtc gtagcgcttc atgatcgtgt tcttggtcga
     1561 gaggtagacc gggtagttgc ggttcagccc gtagttcatc gaggcgcggg cgaaatcccg
     1621 gatcgagtcg tcgaggttgt acatcgccat cgcgatgccg ccgctcggga agtcgtagac
     1681 gtcgagctcg atcggctcag agccgtcctt gggggtgtag gtcagggtca gctggccctc
     1741 accggggacg accaggtcgg tggcgcggta ctggtcaccg aaggcgtgac ggccgatcac
     1801 gatcggcttg gtccaacccg gcaccaggcg cgggatattc gagatcacga tcggctcgcg
     1861 gaagatcacc ccgccgagga tgttgcggat cgtcccgttc ggtgagcggt acatctcctt
     1921 caggccgaac tcctcgaccc tggcctcgtc cggcgtgatc gtcgcgcact tgacgccgac
     1981 cccgtgcttt ttgatcgcgt tggcggcatc gacggtgatc tggtcgtcgc tcgcatcgcg
     2041 gctctcgatc ccgaggtcgt agtactcgag ctcgacatcg aggtagggca ggatcagctg
     2101 ctctttgatg aacgaccaga tgatccgggt catctcgtcg ccgtcgagct cgacgaccgg
     2161 gttctggacc ttgatcttcg ccatcggcgc gcgatgctat ctcgccgggg acttcggctg
     2221 cactgccgtc ctcgacagct tggagctggc acaatcggta cccaatggca accagggagc
     2281 gtcagacctt cggcaaccac gaggttgcca accagccgcc gccgctgagc ggctacaacg
     2341 tcttcgaggc ggacaccccg ctgcgcgagg cggtgcgccg cgagggagcc gagtgggcgg
     2401 agccgcggat cgccgcggcc ggcgcctacg cgggcagcgc ccacgcccag gagctcggcc
     2461 ggctcgccaa cgacaacggc ccgaagctga agacccacga ccgctacggc aaccgggtcg
     2521 acgaagtcga gttccatccc gcctggcacg agctgctcgg caccgcggtc gagcacgaac
     2581 tgcacagcgc gccctggaag gaccccaggc caggcgccca cgtcgcccgt ggcgccgcct
     2641 ttatgtgcat gtcgcaggcg gaggccggga tcggctgccc gatctcgatg acctactcgg
     2701 tg
//
LOCUS       DASRIG010000392         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_197556, whole genome shotgun sequence.
ACCESSION   DASRIG010000392 DASRIG010000000
VERSION     DASRIG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197556"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFB52_10465"
     CDS             <1..447
                     /locus_tag="VFB52_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZO06800.1"
                     /translation="DVVIGAVLVPGASAPKLVTRAMLKSMRPGAVLVDVAIDQGGCFE
                     TSRPTTHGEPTFEVDGITHYCVANMPGAVPITSTHALTNATLPYLMRLAEQGVEHALG
                     FDPGFAAGLNVAAGQLTNEAVARDQGREFLSPARALGAEDAATIPA"
     gene            complement(830..1033)
                     /locus_tag="VFB52_10470"
     CDS             complement(830..1033)
                     /locus_tag="VFB52_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06801.1"
                     /translation="MLGDLARPELSKDAWLDFRDWVEAGGGDYDGFQYRLKTGDAMQD
                     GPFAVLVKDTLLRAPPPAGVVLA"
     gene            1524..2147
                     /locus_tag="VFB52_10475"
     CDS             1524..2147
                     /locus_tag="VFB52_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZO06802.1"
                     /translation="MSRRQEAKGREEHEVEFARIVAFSDGVFAIAITLLVLNLNLDDV
                     ARDEVWQGIWDQRENFLAYAISFAVIGRFWLVHHRFFGEVTAFDGRLLSLNMLYLAFI
                     VLIPFSSQVLGDFGGETASVVVYAVNLIACILVGMWMFVDAQRAGLTSADAVGRREAV
                     VRGCYIAGVFLLSIPVAAVAPNWAPLLWLALFFDPGDRVARAARADR"
     gene            complement(2154..2534)
                     /locus_tag="VFB52_10480"
     CDS             complement(2154..2534)
                     /locus_tag="VFB52_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06803.1"
                     /translation="MRLAIPLTIVAVAAALLSGCGGSDDGDSAASTQAAPKAKGSTAP
                     VGASAHECKGEGELRATGLPCKKARSLMTAWGRTEGCAPIDGASRSACGLSGAYRCLS
                     VVTEKGISVSCAKPGRSLAFTKPG"
     gene            complement(2549..3655)
                     /locus_tag="VFB52_10485"
     CDS             complement(2549..3655)
                     /locus_tag="VFB52_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HZO06804.1"
                     /translation="MRRPRIKLTTEPVDTPEGDLILMRPHTDKDVTIQQPSERDRALL
                     AALDGERTMAQLESEFGAAEVGDTIAQLDELGVIEDAAAYDQLAPEVLTRFDRQLRYF
                     SDVGGRELTPAERQDRLREAKVAVLGVGGLGTWSAWALACCGIGEMWLIDGDRVEIDN
                     LNRQIFFAEADLGRPKVQVAAARLRGFNSSMKVTATNRRLESEVELADFIAGADLVID
                     AADWPAFAFERWCNAACFELGIPYITMSHFPPIARVGPLYVPGETGCYSCQEIAYRRE
                     YPLFDAVTEQQEGRSCPCGTLGPACAKICGEVAMDAVHLLTGLARPATQGVGLIYDLM
                     TMELKQREQVVPEPECPVCAHLQPGVAVKEAAGG"
     gene            complement(3777..4490)
                     /locus_tag="VFB52_10490"
     CDS             complement(3777..4490)
                     /locus_tag="VFB52_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZO06805.1"
                     /translation="MSKGENPTNSVRDRSLPALAETVHGCRLCARLVEWREAVAADPP
                     KRHRSEKYWGRPISGFGDPAARLVIVGLAPAANGANRTGRIFTGDRSGDWLFAALHRA
                     GYANQAESERRDDGLRLRDAYITAVVRCAPPTNKPTPQERDTCLPYLERELELLGEAR
                     VIVALGSFAWDGALRAARALGADVPRPRPRFGHGAEADLGRWRLLGCFHPSQQNTFTG
                     RLTVSMIDAAFARARELCA"
     gene            4507..5124
                     /locus_tag="VFB52_10495"
     CDS             4507..5124
                     /locus_tag="VFB52_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06806.1"
                     /translation="MRALAARRALFLLAVCFVLALAFTACGGGGESTATTESTATTET
                     TAATVPLEAEAEREADIDTLNGILGRQRKAITSISQLIADMRGRPRALAIRIRTQEEE
                     HTLGILERLRALEGSEEVEAEKVEPEGGLDEAERLTRIYETQSATIEAELAAIERLYN
                     TTARSLLAATAANQAQQLVLLRRALGAKPAEWVPVPFENGTTPAP"
     gene            5139..>6823
                     /locus_tag="VFB52_10500"
     CDS             5139..>6823
                     /locus_tag="VFB52_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO06807.1"
                     /translation="MTGPLYAIGRFCSRHHWPVIGVWILLAVALVAISSAAGSKTSEN
                     LTLPGTGSTIATELLEENLPEQAYGSNPLVFEAPAGKKLTEPKYAAAIEESVKKLEAL
                     PDVNSAQSPLVEGSKTISKDQRIAYIPVVLGVGPGEINEEESQEILDAANPALKVGVN
                     TSIGAYVGQQLSKPETEFAELVGLIAAVIILLFAFGTATAMMLPIVSALVGLACALSI
                     IKLLEHALDIPGVAATLATMIGLGVGIDYALFIVTRHKIQLKEGMELRESIARSVATA
                     GGAVVFAGFTVIIALCSLAFAGIPLVSTLGFTAAIAVVVAVCAAATLLPAMLGALGPR
                     INSLRVHFGKTHPDDKKPHGWLRWADAVADRPWRSAIASMAVLIVLAIPLLQLDLGQN
                     DISALPKDSTSREAFEGMDEGFGPGAYGPLLVASEFSSPEEAKQVLPKLEEAIGGAGD
                     VAAVSEPTLDKAGTVAVFTVISKSEPWKDETVSLVENLRDEVIPAALKGTQASSYVGG
                     QTAGYIDLATKIADKLPLMIAIVVGLSFIVLLIAFRSLLVPIKAAAMNLISVAA"
BASE COUNT         1074 a         2376 c         2285 g         1088 t
ORIGIN      
        1 gacgtcgtga tcggggcggt gctggtgccg ggcgccagcg caccaaagct cgtcacgcgc
       61 gcgatgctca agtcgatgcg gccgggcgcc gtgctggtcg acgtcgcgat cgaccagggc
      121 ggctgcttcg agacctcgcg cccgacgacc cacggggagc cgaccttcga ggtcgacggg
      181 atcacccact actgcgtcgc caacatgccc ggcgcggtcc cgatcacctc gacccacgcg
      241 ctcaccaatg cgacgctgcc ctacctgatg cggctggccg agcagggggt cgagcatgcc
      301 ctcggcttcg atcccggctt cgccgccggc ctcaacgtcg ccgccggcca gctgaccaac
      361 gaggctgtgg cccgggacca ggggcgggag ttcctgtccc ccgcgcgggc gttgggggct
      421 gaggacgccg caaccattcc ggcatagcga tctgggcgcc ggaggggtcg agtcccggtc
      481 cgcggcccgg ttgccataag agggggttat cgggcgttcg ccgatcgcgg caagccctgt
      541 ttctgctcag tcgcttcgcc aactacgtcg acccctacga cattgctcgg cgtgaccggc
      601 cgcggtgaaa tcccgtacga agtagagagg tccagacttc ccccgagaag gcgagcacgg
      661 ataaaccaga tcgcgggccc gattgcccgg ctcaaccccg gctcgcctga cggtgacgat
      721 gcagggatcg gtcgcggcct tgaagcgcgc ttctaggtct ggggggctgc cgaagcgcgt
      781 gcggtggcat cggcagatgt cctgcacgat ctcaggacaa tccagatagt catgcgagca
      841 cgactcccgc gggcggagga gcgcggagga gcgtgtcttt aaccaagacg gcgaacggtc
      901 catcctgcat cgcgtccccc gtcttcagtc gatattggaa gccgtcgtag tcgccaccgc
      961 cggcctctac ccagtctctg aaatcgagcc aggcgtcttt gctcaactcc ggccgcgcca
     1021 ggtcgccaag catttcccag atggccttca ggcgttggct gagtggctga aggccacagc
     1081 gcctcacgct ctcgggatcg gttagtcggg ttccgtggaa gagcagcgcg gggggaggtt
     1141 caggagtggc tggggcacgc gaaccttgct acgacctcgg agcgctattc gcagtaccgc
     1201 caactccgcg atgccccacg acgggctgcg gcaagttttc ggccgcggac ggtaaccctc
     1261 gagcagcggt tgctgcctga ccgcggtcgc ctcggagggg tggccgtccc tcattcgtcc
     1321 ctcgaacttc tccggactcc tcggaaacgt cgggacagat caggacaaac gacgcctggt
     1381 gggaagccaa tgtcgacggg tcacgcccag atgcccttca ttacgaatga agtgctctac
     1441 caactgagct acgccggcgc gcccggccat tgtaggaggg atcgccgcgc ggttccgtcg
     1501 cggccggcct cgatgatgcc gggatgtcac gcaggcaaga ggcgaaaggg cgcgaggagc
     1561 acgaggtcga gttcgcgcgg atcgtcgcct tcagcgacgg cgtcttcgcg atcgcgatca
     1621 cgctgctggt gctgaacctg aacctcgacg acgtcgcccg cgacgaagtc tggcaaggga
     1681 tctgggacca gcgggaaaac ttcctcgcct acgcgatcag cttcgccgtg atcgggcgct
     1741 tctggctcgt ccaccatcgc ttcttcggcg aagtgaccgc gttcgacggg cggttgctct
     1801 cgctcaacat gctctacctc gccttcatcg tgctgatccc gttcagcagc caggtgctcg
     1861 gtgacttcgg cggcgagacc gcttcggtgg tggtctacgc ggtcaacctg atcgcctgca
     1921 tcctggtcgg catgtggatg ttcgtcgacg ctcagcgggc cggactcacc tccgccgacg
     1981 ccgtcggcag gcgggaagcg gtcgtgcgcg gctgctacat cgctggcgtc ttcctgctct
     2041 cgatcccggt tgccgctgtc gctcccaact gggcgccgct gctctggctg gcgctcttct
     2101 tcgaccccgg cgaccgggtc gcgcgcgccg ctcgggccga tcgctgagcg cgctcagccg
     2161 ggcttcgtga aggcgagcga gcgcccgggc ttggcgcagc tgaccgagat gcctttctcg
     2221 gtgaccacgc tgaggcagcg gtatgcgccc gagagcccgc aggccgagcg cgaggccccg
     2281 tcgatcggcg cgcagccctc ggtccgcccc caggcagtca tcagcgagcg agccttcttg
     2341 cagggcaggc cggtcgcccg cagttcgccc tcgcccttgc attcgtgcgc cgaggcgccg
     2401 accggcgcgg tcgagccctt cgccttcggc gcggcctgcg tgctcgcggc agagtcgccg
     2461 tcgtcagagc cgccgcagcc actcagcagg gcggcggcga cggcgacgat cgttaagggg
     2521 atggcgagcc tcatcggtcg cccccaactt acccgcctgc cgcctccttc accgcaaccc
     2581 ccggctgcag gtgcgcacag accggacact ccggctcggg tacgacctgc tcgcgctgct
     2641 tcagttccat cgtcatcagg tcatagatga gcccgacgcc ttgggttgcc ggtcgggcga
     2701 ggccggtgag caggtggacg gcgtccatgg ccacctcgcc gcagatcttg gcgcaggccg
     2761 gtccgagcgt cccacacggg cacgagcggc cttcctgctg ctcggtgacc gcgtcaaaca
     2821 gcgggtactc gcgccggtag gcgatctcct ggcaggagta gcagccggtc tcgcctggta
     2881 cgtagagcgg gccgacccgg gcgatcggcg ggaagtggct catcgtgatg taggggatgc
     2941 cgagctcgaa gcaggccgcg ttgcaccaac gctcgaaggc gaaggccggc cagtcggcgg
     3001 cgtcgatcac caggtccgcg ccggcgatga agtcggccag ctcgacctcg ctctccagtc
     3061 gccggttggt cgcggtcacc ttcatcgacg agttgaagcc gcgcagccgt gccgccgcga
     3121 cctggacctt gggccggccc aggtccgcct cggcgaagaa gatctgccga ttcaggttgt
     3181 cgatctcgac ccggtcgccg tcgatcagcc acatctcgcc gatcccgcag caggcgagcg
     3241 cccaggccga ccaggttccg agcccaccga cgccgagcac cgcgaccttc gcttcgcgca
     3301 gtcggtcctg gcgctcggcc ggggtcagct cgcggccgcc gacgtcgctg aagtagcgca
     3361 gctggcgatc gaagcgggtc agcacctcgg gcgccagctg gtcgtaggcg gccgcgtcct
     3421 cgatcacgcc cagctcgtcg agctgggcaa tcgtgtcgcc gacctcggcg gcgccgaact
     3481 cggactccaa ctgagccatt gtgcgctcgc cgtcgagcgc cgccagcagc gcccggtcgc
     3541 gctcgctcgg ctgctggatc gtcacgtcct tgtcggtgtg cggtcgcatc aggatcaggt
     3601 cgccttctgg cgtgtcgacc ggttccgtcg tcagtttgat gcgtggtcta cgcacgcgcc
     3661 cccctcagat tcagttgtcc ccccagtgac tgccctgggg gcaggcacct ggcctttgct
     3721 tgctgcagac ctgcactttg atggaaaggg ttccggtagg tcagcgcggc tgagcgtcag
     3781 gcgcagagtt cacgtgcgcg ggcgaaggcg gcgtcgatca tcgacacggt cagccggccg
     3841 gtgaaggtgt tctgctggct cgggtggaag cagccgagca ggcgccaacg gccgaggtcg
     3901 gcttcggcgc cgtgtccgaa gcgtggccgc ggtcgcggca cgtcggcgcc gagcgcgcgg
     3961 gcggcgcgca gcgctccgtc ccaggcaaag gagccgagcg cgacgatcac ccgggcctcg
     4021 cccagcagct ccagctcgcg ctccagatag gggaggcagg tgtcgcgctc ctgcggcgtc
     4081 ggcttgttgg tcggtggcgc gcagcggacc acggcggtga tgtaggcgtc gcgcagccgc
     4141 aggccgtcgt cgcgccgctc ggactcggct tgattggcgt agccggcccg gtgcaaagcc
     4201 gcgaagagcc agtcgccgga gcggtcgccg gtgaagatgc ggccggttcg gttggccccg
     4261 ttcgcggccg gggcgaggcc gacgatcacc agccgcgcgg ccgggtcgcc gaagccggag
     4321 atcggccgtc cccagtactt ctcgctgcgg tggcgtttgg gcggatcggc ggcaaccgcc
     4381 tcgcgccact cgaccagccg cgcgcagagg cggcagccgt gaacggtctc ggcgagggca
     4441 ggaagcgacc gatcgcgtac cgagttcgta gggttctcgc ccttgctcat tgcaacgaac
     4501 cgaatcatgc gcgctctcgc cgcgcgccgc gccctgttcc tccttgccgt ctgcttcgtg
     4561 ctggcgctgg cgttcaccgc ctgcggtggg ggcggggaga gcaccgcgac gaccgagtcg
     4621 acggcgacga ccgaaacgac cgcagccacc gtgccgctcg aagcggaagc cgaacgcgaa
     4681 gccgacatcg acaccctgaa cgggatcctc ggccgccagc gcaaggcgat cacgtcgatc
     4741 tcccagttga tcgccgacat gcgcgggcgc ccgcgcgcac ttgcgatccg catccgcacc
     4801 caggaagagg aacacacgct ggggatcctc gagcggctgc gcgcgctcga aggcagcgaa
     4861 gaagtcgagg ccgagaaagt cgagcccgaa ggagggctgg acgaagccga acggctgacc
     4921 cggatctacg aaacccagag cgcgacgatc gaagccgagc tggcggcgat cgagcggctc
     4981 tacaacacga cggcgcgctc gctgctcgcc gccaccgctg ccaaccaagc ccaacagctc
     5041 gtccttctgc gccgcgcgct cggcgcaaaa cccgccgaat gggtgccggt cccgttcgag
     5101 aacggcacga cgcccgcccc gtaagagatg attaaccggt gaccgggccc ctgtacgcga
     5161 tcggccggtt ctgctcgcgc caccactggc ccgtgatcgg ggtctggatc ctgctcgccg
     5221 tcgcgctcgt cgcgatctcc tcggcggccg gctcgaagac gagcgaaaac ctgaccctgc
     5281 cggggaccgg ctcgacgatc gcgaccgaac tgctcgaaga gaacctgccc gagcaggcct
     5341 acggctcaaa cccgctggtc ttcgaagcgc ccgccggcaa aaagctgacc gaaccgaagt
     5401 acgccgccgc gatcgaagaa tcggtgaaga agctggaagc cctgccggac gtcaactcgg
     5461 cgcagagccc gctggtcgaa ggctcgaaaa cgatcagcaa agaccagcgg atcgcctaca
     5521 tcccggtcgt gctcggggtc ggtccgggcg aaatcaacga agaggaatcg caggaaatcc
     5581 tcgacgccgc caacccggcg ctcaaggtcg gggtcaacac ctcgatcggc gcctacgtcg
     5641 ggcagcagct ctccaaaccc gagaccgagt tcgccgagct cgtcggcctg atcgcggcgg
     5701 tgatcatcct cctgttcgcc ttcggcacgg cgacggcgat gatgctgccg atcgtctcgg
     5761 cgttggtcgg ccttgcctgc gcgctctcga tcatcaagct gctcgagcac gcgctcgaca
     5821 tccccggcgt agccgccacc ctggcgacga tgatcggcct cggggtcggc atcgactacg
     5881 cgctgttcat cgtcacccgg cacaaaatcc agctcaaaga agggatggag ctgagggagt
     5941 cgatcgcccg ctccgtcgcc accgccggtg gcgccgtcgt cttcgccggt ttcaccgtga
     6001 tcatcgccct ctgctcgctt gccttcgccg ggatcccgct ggtctcgacc ctgggcttca
     6061 ccgccgcgat cgcggtcgtt gttgctgtct gcgccgccgc gacgctgctg ccggccatgt
     6121 tgggggcgct cggaccccgg atcaacagcc tccgcgtcca cttcggcaag acccatccgg
     6181 acgacaagaa gccgcacggc tggctgcgct gggccgatgc ggtcgccgac cggccctggc
     6241 gctcggcgat cgcctcgatg gccgtgctca tcgtcctcgc gatcccgctc ctgcagctcg
     6301 accttgggca gaacgacatc agcgccctgc ccaaggactc gacctcgcgc gaggccttcg
     6361 aaggaatgga cgaaggcttc ggcccgggcg cctatgggcc acttctggtc gcctccgaat
     6421 tcagctcccc cgaagaagca aaacaggtcc tgcccaaatt ggaggaagca atcggcggcg
     6481 ccggcgacgt cgctgcggtc tccgaaccga ccctcgacaa agcgggcacg gtggcggtct
     6541 tcacggtgat ctcgaaatcg gagccgtgga aggacgagac cgtcagcctg gtcgaaaacc
     6601 tccgcgacga agtcatcccc gcggccctca aggggacgca ggcgagctcc tacgtcggtg
     6661 gccagaccgc cggctacatc gacctcgcga ccaagatcgc cgacaagctg ccgctgatga
     6721 tcgcgatcgt cgtcgggctc agcttcatcg tcctgctgat cgccttccgc tcgctgctgg
     6781 tgccgatcaa ggccgccgcg atgaacctga tctcggttgc cgc
//
LOCUS       DASRIG010000393         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3056273, whole genome shotgun sequence.
ACCESSION   DASRIG010000393 DASRIG010000000
VERSION     DASRIG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056273"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VFB52_10505"
     CDS             complement(<1..1488)
                     /locus_tag="VFB52_10505"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZO06808.1"
                     /translation="MFDVTRVSADVGDSQISFETGKLAKQASGAVVVRAGDTMVLCTA
                     TIGSLRDVDFLPLTVDIEERMYAAGKIPGSFFRREGKSSEKATLTARMIDRPIRPLFP
                     KGWHYETQLVAIPMSVDQVNPYDVLAMNGASAALAISPVPVAAHVGAVRIGKLEGEFV
                     VNPEEETHEELELDLIVAGTDEAILMVEAGATGVTEREILDALDIAHGEIKKLTAAME
                     ELQQKVGKEKLEIEAPSIDEGLLSDIRGSHGQALIDAIATEGKLERYEAIDQVKDEVV
                     AKYAPETGDEEADSARKSEVGTAFDAIEKDTIRKAIAVDKKRPDGRAQDEIRPIETEV
                     DISPRVHGSALFTRGETQILSNVALGTTRMDMRIDTLGLQTTKRFWHHYNFPPFSVGE
                     AGFMRGPKRRDIGHGALAERALAASIPDEESFPYVIRVVSETLESNGSSSMGSVCASS
                     MALQAAGVPVSAPVAGVAMGLIKEGDDYIVLTDIAGVEDHLGDMDF"
     gene            complement(1599..2042)
                     /locus_tag="VFB52_10510"
     CDS             complement(1599..2042)
                     /locus_tag="VFB52_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZO06809.1"
                     /translation="MSELTSTAVPPLGPDDHVRGAGETVVVYADLGCPHCAGAWQRLV
                     AEPQRIIFRHFPVSSKHPRAPALHMAAEAAGLQGKFFEMVDSLYADRGRVDDPHLWQR
                     AERFGLDLERFEADRHSEATRRRVRRDFESGIRAGVTGTPAVFPL"
     gene            complement(2065..2925)
                     /locus_tag="VFB52_10515"
     CDS             complement(2065..2925)
                     /locus_tag="VFB52_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06810.1"
                     /translation="MAAGEVTIGADVNGAFNGIGACSGGGNPANRPCTIVVTEYLDRQ
                     MTAPCTGTVTRFRLNGIPTNNTYRLRVVRANGDDTATGTATSAAVSIATEGVNTFATN
                     LPIKIGEFVGVDFQDSTEGGLRYLFLPDGLTEFYFYKFPADGIPGSPTGDESSSYLFN
                     ADVLCDGEGVPPPPPGPPSNAFKVVSLKGKKLTLELASAGAVTVANPATPKRAKGKAK
                     PKLLKRSSASGGPGLVTVPLKLTGAAKAKLEEAGKVKVKAVISFTPNGGATATLTKGF
                     AVKAPKKPAK"
     gene            complement(3078..>3408)
                     /locus_tag="VFB52_10520"
     CDS             complement(3078..>3408)
                     /locus_tag="VFB52_10520"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZO06811.1"
                     /translation="PVCSDQLSIYQEVKPELAEKGVELLGISIDHPYAHKAFQEKLGI
                     DTTLLADFEPKGEVAKAYGSYIDGAGIANRTLVLVGEDGTVAWTYESPTPGEFPGANV
                     IFDGLAS"
BASE COUNT          490 a         1093 c         1211 g          614 t
ORIGIN      
        1 gaagtccatg tcgccgaggt ggtcctcgac gccggcgatg tcggtgagga cgatgtagtc
       61 gtcgccctcc ttgatcaggc ccatggcgac accggcgacc ggtgcgctga ccgggacgcc
      121 cgccgcctgc agtgccatcg aggaggcgca gaccgagccc atcgaggagg acccgttgga
      181 ctcgagcgtc tcggagacga cccggatcac gtacgggaac gactcttcgt ccgggatcga
      241 ggcagccagg gcccgctcgg cgagcgcgcc gtgaccgatg tcacgccgct tcgggccgcg
      301 catgaagccg gcctccccga ccgagaaggg cgggaaattg tagtggtgcc agaagcgttt
      361 ggtcgtctgc aggccgagcg tgtcgatccg catgtccatc cgcgtcgtgc ccagcgccac
      421 gttgctgagg atctgggtct cgccgcgggt gaagagggcc gagccgtgca cgcgcgggga
      481 gatgtcgacc tcggtctcga tcggccggat ctcgtcctgg gcgcgaccgt cgggccgctt
      541 cttgtcgacc gcgatcgctt tgcggatcgt gtccttctcg atcgcgtcga aagccgtgcc
      601 gacctcggac ttacgggccg agtcggcctc ctcgtcaccg gtctccggtg cgtacttggc
      661 gacgacctcg tccttgacct ggtcgatcgc ctcgtagcgc tcgagcttgc cctcggtcgc
      721 gatcgcgtcg atcagagcct ggccgtggga gccgcggatg tcggaaagca aaccctcgtc
      781 gatgctcggg gcctcgatct cgagcttctc cttgccgacc ttctgctgga gctcctccat
      841 cgcggcggtc agcttcttga tttcgccgtg ggcgatgtcg agggcgtcga gaatctcgcg
      901 ctcggtgacg ccggtggcgc cggcctcgac catcaggatc gcctcgtcgg tgccggcgac
      961 gatcaggtcg agttcgagct cctcgtgcgt ctcctcctcg gggttgacga cgaattcgcc
     1021 ttcgagtttg ccgatgcgga ccgcgccgac gtgggcggcg accggaaccg gcgagatcgc
     1081 cagcgccgcg gaagcgccgt tcatcgcgag aacgtcgtag gggttgacct ggtcaaccga
     1141 catcgggatc gcgaccagct gcgtctcgta gtgccagccc ttcgggaaga gcgggcggat
     1201 cggacggtcg atcatgcgcg cggtcagcgt cgccttctcg ctcgatttgc cctcgcgacg
     1261 gaagaacgag ccggggattt tgcccgcggc gtacatccgc tcctcgatgt cgacggtcag
     1321 gggcaggaag tccacgtcgc gcaggctgcc gatggtggcg gtgcagagca ccatggtgtc
     1381 gccggcccga actacgaccg cgccactcgc ctgctttgcg agtttgcccg tctcgaagga
     1441 aatctggctg tcgccgacgt ccgccgagac gcgggttacg tcaaacatgc tcattgagtt
     1501 tttctacctc cggccgcccg gatggcgacc catttgcttt cagaactggc gttctcgtgt
     1561 aggacaaggg tacataccgc gcgcaccgcg gggttccttt acaggggaaa gactgccggg
     1621 gtaccggtga cgccagcccg aatcccggac tcgaagtcgc gccgcacccg gcggcgggtc
     1681 gcctcggagt ggcggtcggc ctcgaagcgc tcgaggtcga gaccgaagcg ttcggcgcgc
     1741 tgccagaggt gcgggtcgtc gacgcggccg cggtcggcgt agagcgagtc gaccatctcg
     1801 aagaacttcc cctgcagccc cgcagcctcg gcggccatgt gcaacgccgg cgcgcgcggg
     1861 tgcttgctgg agaccgggaa gtggcggaag atgatccgct gcggctcggc gaccaggcgc
     1921 tgccaggcgc cggcgcagtg cgggcagccg aggtcggcgt agacgacgac tgtctccccc
     1981 gccccgcgca cgtggtcgtc ggggccgagc ggcggtacgg ccgtgctggt cagctcactc
     2041 acgccgaaag cgtgacaggg gcggctattt ggccggtttt ttcggcgcct tgaccgcgaa
     2101 gcctttggtg agagtcgcag tggcgccacc gttcggcgtg aacgagatca ccgctttgac
     2161 cttgaccttg cctgcttctt cgagcttcgc tttggcggcg ccggtcagct tcagcggcac
     2221 cgtgaccagg ccaggcccgc cgctagcgct ggagcgcttc agcagcttcg gcttcgcttt
     2281 gcctttggct cttttcggag tcgcagggtt ggcgacggtg accgcgccgg ccgaggccag
     2341 ttcgagggtg agcttcttgc ctttgaggct gacgaccttg aaggcgttgc tgggtggacc
     2401 tgggggtgga ggcgggaccc cttcgccgtc gcaaagcacg tcggcgttga agaggtacga
     2461 gctcgactcg tcgccggtcg gcgagcccgg aatgccgtcg gcggggaatt tgtagaagta
     2521 gaactcggtc aaaccgtcgg gcaggaagag gtagcgcagg ccgccttcgg tcgagtcctg
     2581 gaagtcgacc ccgacgaatt cgccgatctt gatcggcaag ttggtcgcga aggtgttgac
     2641 gccttcggtc gcgatcgaga ccgccgcgct ggtcgcggtc ccggtggcgg tgtcgtcgcc
     2701 gttggcgcgg acgacgcgca gccggtaggt gttgttggtc gggatgccgt tgagccggaa
     2761 gcgcgtcacc gtgcccgtgc acggcgccgt catctgccgg tcgaggtact cggtaacgac
     2821 gatcgtgcag ggccggttgg cgggattgcc accgccgcta caggcgccga tcccgttgaa
     2881 ggcgccgttg acgtcggcgc cgatcgtcac ctcgcccgcg gccatcgggg caatgccgag
     2941 cgcgcccagc gcggcgccag ccgtggccgc cagccacgcg gtgcccctgc gtccgcgctt
     3001 tctcccccaa gaagctatgc gggcagcgtt tcacgctgcg gatggatcgt caatacggca
     3061 ggcgccgtag gcgcgctcta ggaggcgagg ccgtcgaaga tgacgttggc gccggggaac
     3121 tcgccggggg tcggcgattc gtaggtccag gcgacggtgc cgtcctcgcc gacgaggacg
     3181 agcgtgcggt tggcgatccc ggcgccgtcg atgtaggagc cgtaggcctt ggcgacctcg
     3241 cccttcggct cgaagtcggc gagcagcgtg gtgtcgatgc cgagcttctc ctggaaagcc
     3301 ttgtgcgcgt aggggtgatc gatgctgatc ccgagcagct cgacgccctt ctcggccagc
     3361 tccggcttca cctcttggta gatcgagagc tggtccgagc agaccggg
//
LOCUS       DASRIG010000394         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1502625, whole genome shotgun sequence.
ACCESSION   DASRIG010000394 DASRIG010000000
VERSION     DASRIG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502625"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFB52_10525"
     CDS             complement(<1..465)
                     /locus_tag="VFB52_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06812.1"
                     /translation="MNAAGLLTRAAVQHGGAEAVRFGERAVTYAELSGRALRLGRALT
                     ELGLEPGDRVALLQANGIEMVESMFGVWAAGMTIVPINSRTHAREAAYVVENCEARAV
                     IYGGEYEDELLAALETSDSLRLISLEAGGRTLSYEDLLAANPPLTRVAERGPD"
     gene            complement(619..2346)
                     /locus_tag="VFB52_10530"
     CDS             complement(619..2346)
                     /locus_tag="VFB52_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06813.1"
                     /translation="MKAAVLEEVGTDLHPGGGDVAPNIPADAFQALFEIGQAVQAEVV
                     ELNRVLSLVVRRTSQLLQADLSWIGLLDEDGTTLRIFDSWGARHPQFEEMAVSMGVGL
                     GGVALRKRETIVAEYYADYTEGTPEMVHNTMLAEGVVSAMCAPMLRGSTMVGALYAAN
                     RQRTHFRPEHVSLLSTLATQVSSAIRHAQMYQELESRNDLLERSYGVHRELTAVGLRE
                     AGLPGIAEALARVVGRALTVEQEVVLPFREEYGAGEPPQTDAEALVVPIAAGDKNLGQ
                     IIVGAAGLSELQILALDHGATVIALELLKQRAARDVELRLRGELLEELLETTGPIPAT
                     IAERAERLQVDIAAPRRVLALEAEGGKIDYGDLLSIVRAKAGRRLHALDGSVLAVKRG
                     GKVIMAITEVPAEVETAILGDIREAVGQREATLAVGISRLAGDFGAAQREATAALQLA
                     KRGNGTDGVMHSDDLGPLRFVLDSTDLTHAEEMVRQQLGPLIDHDAARKSPLMPTLRA
                     YLDANGHQPTAAAACYIHISTLKYRLKKIRELLDEDLSDPETGFQLRLAFKLMDLQEA
                     LGVSTPAGN"
     gene            complement(2354..2743)
                     /locus_tag="VFB52_10535"
     CDS             complement(2354..2743)
                     /locus_tag="VFB52_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HZO06814.1"
                     /translation="MTALLLENEAWAALERIYDPCSVATRSPLNIVDMGLVREVEVDE
                     EHNVRVSICPTSPSCMLMASITEGAEKELRTIEGAAEVEVRIDSEFFWTPEAMTERGQ
                     AELVRHRAARSAEAPVPLGRLKQRTAT"
     gene            complement(2740..3834)
                     /locus_tag="VFB52_10540"
     CDS             complement(2740..3834)
                     /locus_tag="VFB52_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZO06815.1"
                     /translation="MLDDIFVINPVAHAYNLLPENIQDNVSAESLRDMLLAMHETWNP
                     PGIGLGAEEQQSDWPLEVLAKTLFLESDVDVAAFHTLRLDSYMKDGLCRHEKTLEAVT
                     KYPDRFLGYVGVDPTAGLDVCLRELDEQLEDIPNPVGLKLYPSQVAPYRYWRMDDPTL
                     AYPLFERTMERGITTVAIHKAAPLGPVPMDPYKIDDVEGAAGAFPDLNFEIIHSGLAF
                     VTETAWALARYPNVYANFEITSSLAVKAPKMFEAVVGEFLMWGGPEKMIFSDGSMVFH
                     SQPILDRIRDLELSDETLETFGIEQWDHEQKRLFLGGNYARVNGIDVEAKKAAIEDDE
                     FSRERRANGLAEPYSHWRKHLAQSGELVSA"
     gene            complement(4039..4425)
                     /locus_tag="VFB52_10545"
     CDS             complement(4039..4425)
                     /locus_tag="VFB52_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZO06816.1"
                     /translation="MRDADERAAALERFSLITGEDGAEMLAALDAVAPDLGRYVVEFG
                     YGEVYARPGLELRERQIAAVAALVTQGAAEPQLTTHIGGALRVGLSEAEVIEVIVHCL
                     PFVGFPRVLNAVATARRVFERERGAE"
     gene            complement(4515..5936)
                     /locus_tag="VFB52_10550"
     CDS             complement(4515..5936)
                     /locus_tag="VFB52_10550"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF009119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZO06817.1"
                     /translation="MASTLAAEDLAFAGAARQAEMVRAGEVSPKELVQLCLDRIARLD
                     PELNSFRKVFAEKALLEAEQAEARVRAGDQRPLLGVPIAIKDEMPVAGEVCTFGTDAF
                     SEPAREDAEQVRRLREAGAIVVGLTLLPELAICGFTESATYGVSRNPWNPQRSPAGSS
                     GGSAAAVAAGLVPIASASDGGGSIRLPAASCGLFGLKPQRGRVSLAPEIDGWHGLSAA
                     NCVARSVLDSALWLDIVAGGSAEPGAPPPPARSYVEAAKTPPGKLRVAWSIKAPRAVA
                     PPIVGDVVKQAVADAANLLGSLGHEVSQRDPDWGNIGNNITARFLRGVSDSVAEVPHP
                     ERLERRTRGFGKLGGLLPDKAYEAALKGRDSDERRVNAIFDSCDVLVMPVMGGTAIPV
                     RRWQGRGALHTVLGMSRFYPFCIPWNHLGNPAMSVPMGIADDGMPTAVQIISRPGDEA
                     TLFSLAAQIEAARPWADARPPIS"
BASE COUNT          929 a         2225 c         2144 g          990 t
ORIGIN      
        1 gtcggggccc cgctcggcga cccgggtgag cggcgggttg gcggcgagaa gatcctcgta
       61 ggagagcgtc cgcccgccgg cctcgagcga gatcagccgc agcgagtcgg acgtctcgag
      121 cgccgccagc agctcgtcct cgtactcgcc gccgtagatc accgcccgcg cctcgcagtt
      181 ctcgaccacg taggcggcct cgcgggcgtg ggtgcggctg ttgatcggga cgatcgtcat
      241 ccccgccgcc cagacgccga acatcgactc gaccatctcg atgccgttgg cctgcaacag
      301 ggcgacccgg tcccccggtt cgagccccag ctcggtcagg gcacggccca ggcgcagggc
      361 ccgtccgctc agctccgcgt aggtgacggc gcgctcgccg aagcgcaccg cctccgctcc
      421 gccgtgctgg acagccgctc tggtgagcag tcccgccgcg ttcactgact gtgtcccggg
      481 acgcccgagc tcaccaatcc catgccgagg atcgtaggtt cgctatgggt atcccatcta
      541 gatcgtcaac ggtcagatct agaggccgtg atcgtcttgg tcagacagca aaccgtgcac
      601 acaccacgtc aaacggtgtt agttgcccgc cggcgttgag acgccgagtg cttcctgcag
      661 atccatcagt ttgaaggcga gtcgcagctg gaacccggtt tcgggatcgg agagatcctc
      721 gtcgagcagc tcgcggatct tcttcaaccg gtacttcaag gtgctgatgt ggatgtaaca
      781 cgccgccgcc gcggtcggct ggtgcccgtt ggcgtcgaga taggcgcgca gggtgggcat
      841 cagcggcgac ttcctcgccg cgtcgtggtc gatcagcggc ccgagctgct ggcgcaccat
      901 ctcttcggcg tgggtgaggt cggtggagtc gagcacgaag cgcagcgggc cgagatcgtc
      961 ggagtgcatc accccgtcgg tgccgttgcc ccgctttgcc agctgcagcg ccgcggtcgc
     1021 ctcgcgctgc gcggcgccga agtcgccggc gaggcggctg attccgaccg ccagggtcgc
     1081 ttcgcgctgg ccgaccgcct cgcggatgtc gccgaggatc gccgtttcga cctcggccgg
     1141 cacctctgtg atcgccatga tcaccttgcc gccgcgtttc accgccagca ccgagccatc
     1201 gagggcgtgc agccgccggc cggctttggc gcggacgatc gacagcaggt ctccgtagtc
     1261 gatcttgccg ccctcggctt cgagcgccag gacccggcgc ggggcggcga tgtcgacctg
     1321 cagccgctcg gcccgctcgg cgatcgtcgc cggaatcggc ccggtggtct ccaacagctc
     1381 ctccagcagc tccccgcgca ggcgcagctc gacgtcgcgc gccgcccgct gcttgagcag
     1441 ctcgagcgcg atcacggtgg cgccgtggtc gagggcgagg atctgcagct cggagaggcc
     1501 agcggcgccg acgatgatct ggccgaggtt cttgtcgccg gcggcgatcg gcacgaccaa
     1561 cgcctcggcg tcggtttgcg gcggctcacc cgcgccgtac tcttcgcgaa acggcaggac
     1621 gacctcctgc tcgacggtca gcgccctgcc gaccacccgg gccagcgcct cggcgatgcc
     1681 cggcagcccc gcctcgcgca agccgaccgc ggttagctcg cggtgcaccc cgtaggagcg
     1741 ctcgagcaga tcgttgcggc tctccagctc ctggtacatc tgcgcgtggc ggatcgccga
     1801 ggagacctgg gtcgccaggg tcgagagcag cgagacgtgc tcggggcgga agtgggtccg
     1861 ctgccggttg gcggcgtaga gcgcgccgac cattgtcgag ccgcgcagca tcggcgcgca
     1921 catcgccgag acgacgccct cggcgagcat cgtgttgtgg accatctcgg gggtgccttc
     1981 cgtgtaatcg gcgtagtact cggcgacgat cgtctcccgc ttgcgcaacg ccaccccgcc
     2041 caggccgacg cccatcgaga cggccatctc ctcgaactgc ggatgacgag cgccccagct
     2101 gtcgaagatc cgcagcgtgg tgccgtcctc atcgaggagc ccgatccagg agaggtcggc
     2161 ctggagcagc tgcgaggtgc gacggaccac caggctgaga acccggttca gctcgacgac
     2221 ctcggcctgg accgcctggc caatctcgaa cagcgcctgg aaggcgtcgg ccgggatgtt
     2281 tggcgcgacg tccccgccgc ccgggtggag gtcggtgccg acttcttcga ggaccgccgc
     2341 cttcatcgct tcctcaggtc gccgtccgct gcttcagccg tcccagcggc accggagctt
     2401 ccgcgctgcg cgcggcgcgg tggcgaacca actccgcctg cccccgttcg gtcatcgctt
     2461 ccggcgtcca gaagaactcg gagtcgatcc tcacctcgac ctctgccgcc ccctcgatcg
     2521 tgcgcagctc cttttccgcg ccttcggtga tcgaggccat cagcatgcag gagggcgagg
     2581 tcgggcagat gctgacccgc acgttgtgct cctcgtcgac ctcgacctcg cggacgaggc
     2641 ccatgtcgac gatgttgagc ggactgcggg tggcgacgct gcaaggatcg tagatccgct
     2701 ccagcgccgc ccaggcctcg ttctcgagta gcagagcggt catgcgctca ccagctcccc
     2761 gctttgcgcg agatgcttgc gccagtgcga gtagggctcg gcgagaccgt tcgcgcgacg
     2821 ctcgcgggag aactcgtcgt cctcgatcgc cgcctttttc gcctcgacgt cgatgccgtt
     2881 gacgcgggca tagttgccgc cgaggaagag ccgcttctgc tcgtggtccc actgctcgat
     2941 gccgaacgtt tcgagcgtct cgtcggagag ctcgaggtcg cggatccggt cgaggatcgg
     3001 ctgcgagtgg aagaccatcg agccgtcgga gaagatcatc ttttccggtc cgccccacat
     3061 caggaactcg ccgaccaccg cttcgaacat cttcggcgct ttgaccgcga gcgacgaggt
     3121 gatctcgaag ttggcgtaga cgttggggta gcgggccagc gcccaggccg tctcggtgac
     3181 gaaggcgagc ccggagtgga tgatctcgaa gttgaggtcc gggaaggccc cggccgcgcc
     3241 ctcgacgtcg tcgatcttgt aggggtccat cgggaccggg cccagcggcg ccgccttgtg
     3301 gatcgcgacg gtggtgatgc ctcgttccat ggtccgttcg aacagcgggt aggcgagggt
     3361 cgggtcgtcc atgcgccaat agcggtaggg ggcgacctgc gaggggtaga gcttgaggcc
     3421 gaccgggttg gggatgtcct cgagctgttc gtcgagctcg cgcaggcaga cgtcgaggcc
     3481 ggcggtgggg tcgacgccga cgtagccgag gaagcggtcg gggtacttgg tgacggcctc
     3541 cagcgtcttc tcgtggcggc agagaccgtc cttcatgtag gagtcgagcc gcagcgtgtg
     3601 gaaggcggcg acgtcgacgt cgctctccag gaagagagtc ttggccagca cctcgagtgg
     3661 ccagtccgac tgctgctcct cggcgccgag gccgatgccc ggcgggttcc acgtctcgtg
     3721 catcgccagc agcatgtcgc gcagggactc cgccgagacg ttgtcctgga tgttctcggg
     3781 cagcaggttg taggcatgcg ccacggggtt gatgacgaag atgtcatcca gcatttattc
     3841 cctcctcggg gcttcgcgtg tccgcgaccc ggggccgggt cgcgctttcc ctccgcgtcc
     3901 aagcacccag agcccaacgc gttgacacga cgttaagccg accctaaccg aactgccatg
     3961 tccgggcgag acaaaagtcg ccccttctgt cgtaccccca ggacaggcag tcccctgccg
     4021 agcacgacca cggctgacct actccgcacc ccgttcgcgc tcgaataccc gccgcgccgt
     4081 ggcgaccgcg ttcagcaccc gggggaagcc gacaaacggc aggcagtgga cgatcacctc
     4141 gattacctcg gcctcgctca ggccgacccg cagcgcaccg ccaatatgcg tggtcagctg
     4201 cggctccgcc gcgccctgcg tgaccagcgc cgccacggcc gcgatctgcc gctcgcgcag
     4261 ctccagcccg ggccgggcat agacctcgcc atatccgaac tcgaccacat agcgccccag
     4321 atcgggcgcc accgcatcca gcgcggccag catctccgcc ccgtcctcgc cggtgatcag
     4381 cgagaaccgc tcgagcgccg ccgccctctc gtccgcatcg cgcatcgccg ttcgaggcta
     4441 ggccagccga ccctcgcccg ccatgtcccc cctggcggga aacagagccc cggttcgtct
     4501 cggccggcgg accgctagga gatcggcgga cgggcgtccg cccaggggcg ggcggcctcg
     4561 atctgtgctg ccagtgagaa cagcgtggcc tcgtcgccgg gccggctgat gatctggacg
     4621 gcggtcggca tgccgtcgtc ggcgatcccc atcggcaccg acatcgccgg gttgccgagg
     4681 tggttccagg ggatgcagaa gggatagaag cggctcatcc cgagcacggt gtggagagcg
     4741 ccgcggccct gccagcgacg caccgggatc gcggtcccgc ccatcaccgg catcaccagc
     4801 acatcgcagg agtcgaagat cgcgttgacc cggcgctcgt cgctgtcccg gcccttcagc
     4861 gccgcctcgt aggccttgtc ggggagcagg ccgccgagct tgccgaagcc gcgggtgcga
     4921 cgttcgaggc gctccgggtg cggaacctcg gcaaccgagt cgctgacccc gcgcaggaag
     4981 cgcgcggtga tgttgttgcc gatgttgccc cagtcgggat cgcgctggct gacctcgtgg
     5041 ccgagcgagc cgagcaggtt ggccgcgtcg gcgaccgcct gcttgaccac gtcgccgacg
     5101 atcggcggcg cgaccgcccg cggggccttg atcgaccagg ccacccgcag cttgcccggc
     5161 ggcgtcttcg ccgcctcgac gtacgagcgc gcgggcggcg gcggcgcgcc gggctcagcc
     5221 gagccgccgg cgacgatgtc gagccagagc gccgagtcga gcacgctgcg ggcgacgcag
     5281 ttggcggcgc tgaggccgtg ccagccgtcg atctcgggcg ccagcgagac gcgaccccgc
     5341 tgcggcttca ggccgaacag cccgcaggag gcagcgggga gtcgaatcga gccgccgcca
     5401 tcggaggccg aggcgatcgg caccaggccg gcggcgaccg cggcggccga gccgccgctg
     5461 gagccggccg ggctgcgctg cgggttccac gggttgcggc tgacgccgta ggtcgccgac
     5521 tcggtgaagc cgcagatcgc cagctcgggc agcagggtca gaccgacgac gatcgccccc
     5581 gcctcgcgca gccggcgcac ctgctccgcg tcctcgcggg ccggctcgct gaaggcgtcg
     5641 gtcccgaagg tgcagacctc gccggcaacc ggcatctcgt ccttgatcgc gatcggcacc
     5701 cccagcagcg gtcgttgatc gcccgcccgc acccgcgcct cggcctgctc ggcttcgagc
     5761 agcgccttct cggcgaagac cttgcggaaa gagttcagct ccgggtccag ccgggcgatc
     5821 cggtcgaggc agagctgcac gagctccttg ggagagacct ctcccgcccg caccatctcc
     5881 gcctgacgcg ccgcccccgc gaacgccaga tcctccgccg ccaaagtact ggccatgcgg
     5941 ccaatcctat gggttcggag agggtttggg gtgaggggtc ggagcggccc ttccgcttaa
     6001 cttttttgtg gggcggatgc ggctttacgc cttgggacac cgaagcaaaa aagtacaagg
     6061 cgctccaggg ccaccccgac ccctccacac aagccgcccc gaagctcctg aagctacctg
     6121 gcggaacctc gttcgctggg agccgatccc ggggcttcag ggaggatggg gtgtcctccg
     6181 tacgagcggc gtccgcgatt cgcaccggcg ctcgatctcg gatggtcgcc caaacgcctc
     6241 tggcgaattc gcccagcgaa ggtaggagga caccccatcc tccctagc
//
LOCUS       DASRIG010000395         5460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2804637, whole genome shotgun sequence.
ACCESSION   DASRIG010000395 DASRIG010000000
VERSION     DASRIG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804637"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="prmC"
                     /locus_tag="VFB52_10555"
     CDS             complement(<1..823)
                     /gene="prmC"
                     /locus_tag="VFB52_10555"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZO06818.1"
                     /translation="MATVREALDASVDGLTAIGVEDARLDAELLLGEATGYERARLTA
                     EPQAELPAGSARTFGEMVRRRLRREPVAYILGTKGFRHIELAVDRRVLIPRPETELLV
                     ELAVERQPRRVLDVGTGSGAIALAVADEVAECEVVATDTSPGALEVARANAARLGLAE
                     RVTFVEGTLPDGEFDLVLANLPYIPEADWPGLQPEVRDWEPREALLAGPDGLDAYRTL
                     ISSPSPQAALHLSPSGRQMQGALAVEVGVGQAEAVGELMRGAGFEAIEVRKDLAGI"
     gene            complement(837..1910)
                     /gene="prfA"
                     /locus_tag="VFB52_10560"
     CDS             complement(837..1910)
                     /gene="prfA"
                     /locus_tag="VFB52_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZO06819.1"
                     /translation="MGTMIEQLVEQIQARFAELEGLMSDPDVIGDRERYAEVGREYRD
                     LQPARELASEWATLKDDLEGAREMLEEGEDEELRKVVEEAPERLAQLEEEIRLAMVER
                     DPNDEKNVIVEIRAGTGGDEAALFAGDLYKMLTRYAEIRGFATEVLSQSASDAGGFKE
                     VTLAVKGDGAYSVFKYEGGTHRVQRVPETESQGRIHTSTATIAVLPEAEEVDVEVDQN
                     DLQIDVYRSSGPGGQSVNTTDSAVRITHKPTGIVVSMQDEKSQLQNKDKAMRVLRARL
                     LEREIAEQQAKIASERKAQVGSGERSEKVRTYNFPQGRVTDHRIKLTSHNLDGVLGGD
                     LSEFTDALSAEEKRQRLEAAAVG"
     gene            complement(1936..2991)
                     /locus_tag="VFB52_10565"
     CDS             complement(1936..2991)
                     /locus_tag="VFB52_10565"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZO06820.1"
                     /translation="MSPPSGLTRASHAPTEGQNGAGHNGAAANGGGDRDVRMPDGALV
                     AKRDAPVGGQAVLEGVMMRGVGVWAVAVRNPEGQIEIETETIKPWAKRHRLWRVPVIR
                     GVVALAESMKIGFKALAISANAQLEEDEDGEKEEIGGWVWGFTIFLSLLLAVGLFFVV
                     PVGLTSLIKDQLGSALLFWLIEGVVRTLIFIGYILAISKMPDLRRVFEYHGAEHKTIS
                     CYEAEDELTPQRAKLYSRLHPRCGTSFLLIVMVLAIFVFAPIGLPEWYWLLASRILGI
                     PLIAGLSYEVIKWAGKNRRKAWVRAVMWPGLMLQNLTTREPDEEQLEVAIASLNAVLA
                     RENPEEAAKQQHIEIVA"
     gene            complement(2996..3208)
                     /gene="rpmE"
                     /locus_tag="VFB52_10570"
     CDS             complement(2996..3208)
                     /gene="rpmE"
                     /locus_tag="VFB52_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZO06821.1"
                     /translation="MKAAIHPNYTLANVHCSCGNQFYTRSTKEDLHVELCSECHPFYT
                     GKQKLVDTGGRVERFQRRAAKHRAGK"
     gene            3321..4790
                     /locus_tag="VFB52_10575"
     CDS             3321..4790
                     /locus_tag="VFB52_10575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZO06822.1"
                     /translation="MLIGGPLLIAAHDWLGVGGGLFGDFVNGAVYAVVVLAAGLACLL
                     KARASVRERAAWVVITAAIFSSALTDTYWAIAFADDPTPPFPSLADIGYLAFYPLAAT
                     GIYLLIRARTRTLDWRLWMDGMIAALGTAAVGAALVFEFVAERASGSPLDVFVTLAYP
                     IGDILLLALVVGVVALTRWQPGRSWALVLLGLTFLLVADITTTMQSAGTGTEAAEDWV
                     KPIFMLGAICIGAEAWQPPARAIPPAARFDGWRELIVPGFFAIVMIVLFSLQHFSQAS
                     TLTTVLWTATMLAVVARFAMSVRENKQLLEQVRTDPLTGLGNQGGMQVDLEGRVKRAA
                     EEPFSLLLLDLNGFKAFNDTFGHPAGDAMLARLGRQFEAAIGDQGEAFRIGGDEFAAL
                     IYNPDPEGVAEVSKLAAEALTAAGEDYELGASWGVVGVPEEARTSAEAMQLADVRMYA
                     QKQSRQLALGPTVTGPNKVASKPPGLEPLGQSEAVEQRA"
     gene            complement(4746..5300)
                     /locus_tag="VFB52_10580"
     CDS             complement(4746..5300)
                     /locus_tag="VFB52_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06823.1"
                     /translation="MAAARELFTERGYENVGTEEIVRAAGVTRGALYHHFGGKAELLE
                     AVYERLEAESTERVAHIVLGSHFESPVEAMKAGIEAFLDECAKPELRQIALHDAPAVL
                     GWDRWREIGAANGLGLIEASLTAAIEAGEIRSLPVKPLAHLLLGALDEAAMLVARDED
                     PASRAEVTATLSALLDGFALSERL"
BASE COUNT          789 a         1972 c         1801 g          898 t
ORIGIN      
        1 cgatcccggc gaggtccttc cgcacctcga tcgcctcgaa tcccgccccc cgcatcagct
       61 caccaaccgc ctccgcctgc ccaacgccga cctcgaccgc cagagcgccc tgcatttgtc
      121 gcccagaggg cgacaaatgc agggcggctt gggggctcgg cgaggagatc agggtgcggt
      181 aggcgtcgag gccgtcgggg ccggcgagga gggcctcgcg gggctcccag tcgcggacct
      241 cgggctggag gccgggccag tcggcctcgg ggatgtaagg gaggttggcg aggaccaggt
      301 cgaactcgcc gtcggggagc gtgccttcga cgaacgtgac gcgctccgcc aggccgagcc
      361 gggcggcgtt ggcgcgagcg acttccagcg ccccgggcga cgtgtccgtc gcgaccacct
      421 cgcactccgc cacctcgtcc gctaccgcca gcgcgatcgc gccggagccg gtgccgacgt
      481 cgaggacccg ccgtggctgg cgctccaccg ccagctcaac gagcagctcg gtctccggcc
      541 gcggaatcaa cacccgccga tcgaccgcca gctcgatgtg acggaagcct ttcgtcccga
      601 ggatgtaggc caccggctcg cgccgcagcc tccgccgcac catctcccca aacgtccgcg
      661 ccgaccccgc cggcagctcg gcctgcggtt ccgccgtcag ccgggcgcgc tcgtaccccg
      721 tcgcctcacc cagcagcagt tccgcatcga gccgcgcgtc ctccacgccg atagccgtca
      781 gcccatcgac cgacgcatcc agggcctcgc gaacagtggc catcgcggtc ggcatctcag
      841 ccgacggccg cagcctctaa gcgctgccgc ttctcctccg cgctcaacgc atcggtgaac
      901 tcgctcaggt caccgcccag caccccgtcg aggttgtgcg aggtcagctt gatccggtgg
      961 tcggtgaccc ggccctgggg gaagttgtag gtccgcacct tctccgagcg ctcgccgctg
     1021 ccgacctggg ctttgcgctc gctggcgatc ttcgcctgct gctcggcgat ctcgcgctcg
     1081 agcagacgcg cgcgcagcac ccgcatcgcc ttgtccttgt tctgaagctg ggacttctcg
     1141 tcctgcatcg agaccacgat cccggtcggt ttgtgggtga tccgcaccgc cgagtcggtc
     1201 gtgttgaccg actgcccgcc gggcccggag gagcggtaga cgtcgatctg caggtcgttc
     1261 tggtcgacct cgacgtcgac ctcctcggcc tcgggcagca ccgcgatcgt cgcggtcgag
     1321 gtgtggatgc gcccctgcga ctcggtctcc gggacccgct ggacccggtg cgtgccgccc
     1381 tcgtacttga acaccgagta ggcgccgtcg cccttgaccg cgagggtcac ctccttgaag
     1441 ccgcccgcgt cggaggccga ctgcgagagc acctcggtgg cgaagccacg aatctcggcg
     1501 tagcgggtca gcattttgta gagatcgccg gcgaacagcg ccgcctcgtc gccgccggtg
     1561 ccggcgcgaa tctcgacgat cacgtttttc tcgtcgttgg ggtcgcgctc gaccatcgcc
     1621 agccggatct cctcttccag ctgggcgagc cgctcgggcg cctcctcgac gaccttgcgc
     1681 agctcctcgt cctcgccttc ctcgagcatt tcccgggccc cctcgaggtc gtccttgagc
     1741 gtcgcccact cgctcgccag ctcacgcgct ggctgcagat cccggtactc gcgcccgact
     1801 tcggcgtagc gctcgcggtc gccgatcacg tccgggtcgg acatcagccc ctcgagctcc
     1861 gcgaagcggg cctgaatctg ttcgacgagc tgctcgatca tcgttcccaa accctagagg
     1921 cgcgcggcct cagagctaag cgacgatctc gatgtgctgc tgtttcgccg cctcctcggg
     1981 gttctcccga gcgaggacgg cgttgagtga ggcgatcgcg acctcgagct gctcttcgtc
     2041 gggctcgcgg gtggtcaggt tctgcaacat cagccccggc cacatcaccg cccgcaccca
     2101 cgctttgcga cggtttttgc ccgcccactt gatcacctcg taggagagcc cggcgatcag
     2161 ggggatcccg aggatccgcg aagccagcag ccagtaccac tcgggcagcc cgatcggggc
     2221 gaagacgaag atcgccagga ccatcacgat cagcaggaag ctggtcccgc agcgcgggtg
     2281 cagacgcgag tagagcttcg cccgctgggg ggtcagctcg tcttcggcct cgtaacagga
     2341 gatcgtcttg tgctcggccc cgtggtactc gaagacccgt cgcaggtcgg gcattttgct
     2401 gatcgcgagg atgtagccga tgaagatcag ggtgcggacg accccctcga tcagccagaa
     2461 cagcagcgcc gagccgagct ggtccttgat caggctggtc agcccgaccg ggacgacgaa
     2521 gaagagcccg accgcgagca gcagcgagag gaagatcgtg aagccccaga cccagccgcc
     2581 gatttcttct ttctcgccgt cctcgtcctc ctccaactgc gcgttggcgg agatcgccag
     2641 ggctttgaag ccgatcttca tcgactcggc gagggcgacg acgccgcgga tcaccggcac
     2701 gcgccagagc cggtggcgct tcgcccacgg cttgatcgtc tcggtctcga tctcgatctg
     2761 gccctcgggg ttgcgcacgg cgactgccca gaccccgacg ccgcgcatca tcacgccctc
     2821 gagcaccgcc tgaccgccga ccggcgcgtc gcgcttggcg accagggcgc catcgggcat
     2881 gcgcacgtca cggtcgccgc cgccgttcgc ggcggcgccg ttgtgcccag caccattctg
     2941 cccctcggtc ggggcgtggg acgcccgcgt caagcccgac ggcgggctca ccggactact
     3001 tcccggccct gtgctttgcg gcgcggcgct ggaaacgctc gacgcgaccg ccggtgtcta
     3061 cgagcttctg cttgcccgtg tagaaggggt ggcactcgga gcaaagctct acgtgcaggt
     3121 cctctttcgt agaccgcgta tagaactgat tgccgcacga acagtgcacg tttgcaagcg
     3181 tgtaattggg gtggattgct gccttcatct ccaccaagtc tagagaactg cgcaagtgct
     3241 gccgatacgc ctctgtgaag cccgccgata acagccaaga aagcgtccct ccgggaccgt
     3301 tacgcacggc catcggggtc gtgctgatcg gcggcccact gctgattgcc gcccacgatt
     3361 ggctaggtgt cggcgggggt cttttcggcg atttcgtcaa cggcgcggtc tacgcggtgg
     3421 tcgtcctcgc cgccgggctc gcctgcctgc tgaaagcgcg ggcgagcgtg cgcgagcgcg
     3481 ccgcctgggt ggtgatcacg gccgcgatct tctcctcggc gctgaccgac acctactggg
     3541 cgatcgcatt cgccgacgac ccgaccccgc ccttcccctc gctggccgac atcggctacc
     3601 tcgccttcta cccgctcgcc gcgaccggga tctacctgct gatccgggcg cggacgcgaa
     3661 cgctcgactg gcgcctctgg atggacggga tgatcgccgc cctcgggacc gccgcggtcg
     3721 gcgccgcgct cgtcttcgag ttcgtcgccg agcgcgccag cggctcaccc ctggacgtgt
     3781 ttgtcaccct cgcctacccg atcggcgaca tcctgctgct ggcgctggtc gtcggcgtcg
     3841 tcgccctcac tcgctggcag ccggggcgca gctgggcgct ggtcctgctc ggcctgacct
     3901 tcctcctcgt cgccgacatc accacgacca tgcagtcggc cggcaccggc accgaagcgg
     3961 ccgaagactg ggtcaagccg atcttcatgc tcggcgcaat ctgcatcggc gccgaggcct
     4021 ggcagccgcc ggcccgcgcg atccccccgg cggcccgctt cgacggctgg cgcgagctga
     4081 tcgttcccgg cttcttcgcg atcgtgatga tcgtgctctt cagcctccag cacttcagcc
     4141 aggcgagcac gctgaccacg gtgctgtgga cggcgacgat gctcgccgtg gtcgcccgct
     4201 tcgcgatgag cgtgcgcgag aacaagcagc tcctcgagca ggtgcggacc gatccgctga
     4261 ccgggctcgg caaccagggc ggcatgcagg tcgacctcga ggggcgcgtc aagcgcgccg
     4321 ccgaggaacc gttcagcctg ctgctgctcg acctcaacgg gttcaaggcc ttcaacgaca
     4381 ccttcggcca tccggccggc gacgcgatgc tggcccggct tggccgccag ttcgaggcgg
     4441 cgatcggtga ccagggcgag gcctttcgga tcggcggcga cgagttcgcg gcgctgatct
     4501 acaaccccga ccccgagggg gtcgccgagg tcagcaagct ggccgcggaa gcgctgaccg
     4561 ccgccggcga ggactacgag ctgggcgcct cctggggcgt ggtcggggtg ccggaggagg
     4621 cgcgcacgtc ggccgaggcg atgcagctcg ccgacgtccg catgtacgcg cagaagcagt
     4681 cgcgccagct cgcgctgggg ccaacggtga ccggaccgaa caaggtcgcc agcaagccgc
     4741 ccgggctaga gccgctcgga cagagcgaag ccgtcgagca gcgcgcttag ggtcgcggtg
     4801 acctcggccc gcgaggccgg gtcctcgtcg cgggcaacca gcatcgccgc ctcgtcaagg
     4861 gctccgagca acaggtgggc gagcggcttg accggcagcg agcggatctc ccccgcctcg
     4921 atcgctgcgg tcagactggc ctcgatcagc cccagcccgt tggcggcccc gatctcgcgc
     4981 cagcgatccc agccgagcac ggcgggtgcg tcgtggaggg cgatctggcg cagctccggc
     5041 ttcgcacact cgtcgaggaa ggcctcgatc ccggccttca tcgcctccac cgggctctcg
     5101 aagtgggagc cgagcacgat gtgggcgacg cgctcggtcg attccgcctc cagccgttcg
     5161 tagaccgcct ccagcagctc cgccttgccg ccgaagtggt ggtagagggc gccgcgggtg
     5221 acaccggcgg cgcggacgat ctcttcggtg ccgacgttct cgtagccgcg ctcggtgaac
     5281 agctcgcggg cggcggccat cagcgccgac cgggtcgcct cggtgcgctc gccctggctg
     5341 cgacgctgtt tccgtgcttt ttgcggcttt ccgggggggt cgggattcac atgcatactg
     5401 cctgtatgtt agaagaacac gaacagattg cgctcataaa tgggaggtgg ccaagaacca
//
LOCUS       DASRIG010000396         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3297401, whole genome shotgun sequence.
ACCESSION   DASRIG010000396 DASRIG010000000
VERSION     DASRIG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3297401"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFB52_10585"
     CDS             <1..396
                     /locus_tag="VFB52_10585"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06824.1"
                     /translation="AMQGKSREELIEESKPDALKDLKREAVVTAIAEAEGIEVTEEEM
                     VEALAHTAEHERTTPEKLLARLRDNGRASMIEEDIKARKAIEVVVEAATPIPLEQEEE
                     EKAKADAREKILTPEGEKAGGKLWTPGDE"
     gene            407..991
                     /gene="clpP"
                     /locus_tag="VFB52_10590"
     CDS             407..991
                     /gene="clpP"
                     /locus_tag="VFB52_10590"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZO06825.1"
                     /translation="MSPLVPMVVEQTSRGERAFDIYSRLLGERIIFLGTPVTDDIANL
                     IVAQLLHLESEDPDKDVSLYINSPGGSVYAGLAIYDTMQFIKPQVQTICVGVAMSMGA
                     LLLSGGAAGKRMALPNSKILIHQVSAGFQGQATDIEIHAKEIIDVRRRLDEIIAHHTG
                     QPFDKVTKDTERDYFMSAEEAKEYNIVDRVIEHH"
     gene            1036..1977
                     /locus_tag="VFB52_10595"
     CDS             1036..1977
                     /locus_tag="VFB52_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06826.1"
                     /translation="MLRFLKISLAVAALCAFAAAPAGAAVTFGSAMLSEPTGAPCATI
                     SEPQSCTYAIGTLPAESQEVGGTVAPSAGVITSWKVKAGPNGSASDEPDLRLRVLRGN
                     TGVGSGPIEELPLGKGIYSYIARLPVQAGDRLGLDFLGVQPGEAPPVAKTMTGATTDT
                     WSPPLADGATLLPTTGTNNDYELMVQATVEPDADNDGWGDESQDKCKGSSGPNEGCPA
                     NNPPAPPIPTEAVPDTKIGKVTIIAGQKKVTFRFTATVANASFQCRLDKKPWKPCKSP
                     RTYKALKSGRHSFKVKAIGPTAVPDPTPAKRGFRLES"
     gene            1974..2942
                     /locus_tag="VFB52_10600"
     CDS             1974..2942
                     /locus_tag="VFB52_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06827.1"
                     /translation="MRRRLGILGAALGLLGVVAAPADAAVVSGSNLALAPNATICVLP
                     GIPNLSCTTAITGLAMASRAPGGARAASDGVIVSWSLRAGASTIDHSVRLRVVRNDTG
                     AGTGPTEVLPKAAGVYTYAARLPVGNGDLLGVDTLNVPEMLGVPIVRSPASGANLDYW
                     SSQLADGETRAPFTNEAALELLMNATIEPDADNDGWGDETQDKCPTGAATGNEGCPPP
                     KKQGPPPPSGESPIPTEPTTPDTKLGKGGPKGTTDKTKATFRFTATVAGSTFQCKLDK
                     KPWKACKSPKTYKGLKEGRHTFKVRAVGVSGLIDTTPAKRAFKVEL"
     gene            2954..3469
                     /locus_tag="VFB52_10605"
     CDS             2954..3469
                     /locus_tag="VFB52_10605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZO06828.1"
                     /translation="MTAACAALLCVALFAVAPAGAKIVSRNGGLTYYGHVFPVQGAHG
                     VRGAVGEYHAPRSGGRIHEGFDITAACGTPLVAVRNGRVRQVGYDPVLYGYFALIHGE
                     GEKRSYFYAHMPGPARVRKGEQVFTGERIGAVGETGNAVGTGCHLHFEIHVNGTPIDP
                     LPSLSRWDRYS"
     gene            complement(3460..3933)
                     /locus_tag="VFB52_10610"
     CDS             complement(3460..3933)
                     /locus_tag="VFB52_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06829.1"
                     /translation="MSSAEIVFETVARDAPEVQALYEPFIREADGPLGEAVDLEAEIA
                     AGPPADLVPPGGVFLLARVGGEPAGIGGVRHLDTDVAEVKSMYVSPAHRGIGLGRRLL
                     AELDRIAADHGCGAVRLDTSDYLSEAIGLYEAAGYREVPDYNGNPKADLWFERQL"
     gene            complement(3930..4373)
                     /locus_tag="VFB52_10615"
     CDS             complement(3930..4373)
                     /locus_tag="VFB52_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06830.1"
                     /translation="MEISPVSPAQFEELLPLIANYQRFYGVENPDTDRNREFFRRFLA
                     PSDDGLLLGARGEGGFLGYACLYWHFSSTRAIEVVLMNDLFVDESARGRGVGRALIEA
                     SATVARERGAPVLEWSTEPGNKTAQRLYDKTGAERSEWVSYEIEL"
     gene            4603..>4679
                     /locus_tag="VFB52_10620"
     CDS             4603..>4679
                     /locus_tag="VFB52_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HZO06831.1"
                     /translation="MAGPGVYICDECIDLCNEIIDEELTA"
BASE COUNT          859 a         1630 c         1561 g          629 t
ORIGIN      
        1 gcgatgcagg gcaaatcccg cgaggagctg atcgaggaat cgaaacccga cgcgctcaaa
       61 gatctgaagc gcgaggcggt ggtgacggcg atcgccgagg ccgagggcat cgaggtcacc
      121 gaggaggaga tggtcgaggc gctcgcgcac accgccgaac acgaacggac gacgcccgag
      181 aaactgctcg cccgcctgcg cgacaacggc cgcgcgtcga tgatcgaaga ggacatcaag
      241 gcgcgcaaag cgatcgaggt cgtggtcgag gcggcgacgc cgatcccgct cgagcaggaa
      301 gaagaggaga aggcgaaagc cgacgcccgc gagaaaatcc tgacgcccga gggcgagaaa
      361 gccggcggca aactttggac gccgggcgac gagtagagtt cggcctatga gtcccctggt
      421 cccgatggtg gtcgagcaga cctcgcgcgg cgagcgcgcc ttcgacatct actcgcgcct
      481 gctcggcgag cggatcatct tcctcggcac gccggtcacc gacgacatcg ccaacctgat
      541 cgtcgcccag ctgctgcacc tggagtccga ggaccccgac aaggacgtca gcctctacat
      601 caactcgccc ggcggctcgg tctatgcggg cctggcgatc tacgacacga tgcagttcat
      661 caaaccgcag gtgcagacga tctgcgtcgg cgtcgcgatg agcatggggg cgctgctgct
      721 gagcggcggt gccgccggca agcggatggc gctgccgaac tcgaagatcc tgatccacca
      781 ggtctccgcc ggcttccagg gccaggccac cgacatcgag atccacgcca aagagatcat
      841 cgacgtgcgc cgccggctcg acgagatcat cgcccaccac accgggcagc cgttcgacaa
      901 ggtcaccaaa gacaccgagc gggactactt catgagcgcg gaggaggcga aggagtacaa
      961 catcgtcgac cgcgtgatcg agcaccacta gcgccgcatc tccggtccgc tatccgggtt
     1021 aggatcaccg acccgatgct ccggttcctc aagatttcgc tcgctgtcgc cgccctctgc
     1081 gcgtttgccg ctgcgccggc cggcgccgcc gtcaccttcg gcagcgcgat gctgagcgag
     1141 cccaccggcg caccctgcgc gacgatctcc gaaccgcagt cctgcaccta cgcgatcggc
     1201 acgttgccgg cggaaagcca ggaagtgggc gggacggttg ctcccagcgc gggcgtgatc
     1261 acctcgtgga aagtcaaggc cggacccaac ggcagcgcca gcgacgagcc cgacctgagg
     1321 ctccgcgtgc tgcgcggcaa caccggtgtc ggcagcggac cgatcgagga gctgccactc
     1381 ggcaaaggca tctactccta catcgcgcgg ctgcccgtgc aggccggcga tcgcctcggg
     1441 ctcgacttcc tcggcgtcca gcccggcgaa gcgccgccgg tggcgaagac gatgaccggc
     1501 gcgaccaccg acacctggag cccgccgctg gcggacggcg ccacgttgct ccccactacc
     1561 gggaccaaca acgactacga gctgatggtt caggcgacgg tcgagcccga cgccgacaac
     1621 gacggctggg gcgatgagtc ccaggacaaa tgcaaaggct ccagcggccc gaatgagggc
     1681 tgcccggcga acaacccgcc cgccccgccg atcccgaccg aggcggtacc cgacaccaag
     1741 atcggcaaag tcacgatcat cgccgggcag aagaaggtca ccttccgctt caccgccacg
     1801 gtcgcgaacg ccagctttca gtgcaggctc gacaaaaagc cctggaaacc atgcaaatct
     1861 ccgagaacct acaaagctct gaaaagcggc cggcacagct tcaaagtgaa agcgatcggg
     1921 ccgaccgcgg ttccggaccc gacgccggcc aaacgcggct tcaggttgga gtcgtgaggc
     1981 gcaggctcgg catcctcggc gctgcgctcg gcctgctggg cgtcgtcgcg gcgccggcgg
     2041 acgcggcggt cgtctccggg agcaacctcg ccctggcgcc caacgccact atctgcgtgc
     2101 tcccgggcat acccaatctc tcctgcacca ccgcgatcac cggtcttgcg atggccagtc
     2161 gggcgcccgg tggcgctcgg gcggcgagcg acggtgtgat cgtctcctgg agcttgagag
     2221 caggcgccag cacgatcgac cactcggttc ggctgcgggt ggtccgcaac gacaccgggg
     2281 cggggaccgg cccgaccgag gtcctgccca aagccgcggg tgtgtacacc tacgcggcgc
     2341 gcctgccggt cggcaacggc gacctgctcg gcgtcgacac gctgaacgtt cccgaaatgc
     2401 tcggcgtgcc aatcgtccgg agccccgctt ccggcgccaa cctcgactac tggagcagcc
     2461 aactggccga cggcgaaacc agggctccgt tcaccaacga ggcggctctg gaactgctga
     2521 tgaacgcgac gatcgagccg gacgccgaca acgacggctg gggcgacgag acgcaggaca
     2581 aatgcccgac cggggcagcg accggaaacg aaggctgccc gccgcccaaa aaacagggcc
     2641 caccgccgcc cagcggcgaa tcgccgatcc cgaccgagcc gaccacgccc gacacgaagc
     2701 tcggcaaagg cggcccgaaa ggcaccacgg acaagaccaa ggcgaccttc cgcttcaccg
     2761 ccaccgtcgc tggctccacc ttccagtgca aattggacaa aaagccctgg aaagcgtgca
     2821 aatcgccgaa gacctacaaa ggcctgaagg aaggtcggca caccttcaaa gtccgcgccg
     2881 tcggcgtctc cgggctgatc gacacgactc cggcaaagcg cgcgttcaag gtcgagctct
     2941 gaacggtcgt tcgttgacgg cggcctgcgc ggcgttgctc tgcgtcgcgt tgttcgcggt
     3001 agctccggcc ggtgccaaga tcgtcagccg caacggcggc ctcacctact acggccacgt
     3061 cttcccggtc cagggagcgc acggcgtgcg cggtgcggtc ggcgaatacc acgcgccccg
     3121 cagcggcggg cgcatccacg aaggcttcga catcaccgcc gcctgcggca cgccgctggt
     3181 cgccgtgcgc aacggccgcg tgcgccaggt cggctacgac cccgtcctct atggctactt
     3241 cgccctgatc cacggcgaag gggagaagcg cagctacttc tacgcccaca tgcccggccc
     3301 cgcccgtgtc cgcaagggcg agcaggtctt caccggcgag cggatcggag cggtcggcga
     3361 aaccggcaac gccgtcggca ccggctgcca cctccacttc gagatccacg tcaacggcac
     3421 cccgatcgac ccgctcccct ccctgtcccg ctgggaccgc tacagctgac gttcgaacca
     3481 gaggtcggct ttggggttgc cgttgtagtc ggggacttcg cggtagccgg cggcttcgta
     3541 gaggccgatc gcctcgctca ggtagtcgga ggtgtcgagg cggacggcgc cacagccgtg
     3601 gtcggcggcg atccggtcga gctcggcgag caggcggcga ccgaggccga tgccgcggtg
     3661 ggcgggggag acgtacatgc tcttgacctc ggcgacgtcg gtgtcgaggt ggcggacgcc
     3721 accgatcccg gccggctcgc cgccgacccg ggcgagcaag aagacaccgc cgggcgggac
     3781 caggtcggcg ggcggccccg ccgcgatctc ggcctcgagg tcaaccgcct cgccgagcgg
     3841 gccgtcggcc tcgcggatga acggctcgta gagcgcctgc acctcggggg cgtcgcgcgc
     3901 caccgtttcg aagacgatct cggcgctgct caaagctcga tctcgtagct gacccattcg
     3961 ctgcgctcgg ccccggtctt gtcgtagagg cgctgcgcgg tcttgtttcc cggttcggtc
     4021 gaccactcga ggacgggagc gccgcgctcg cgggcgacgg tggcgctcgc ctcgatcagc
     4081 gctcggccga cgccgcggcc gcgggcggat tcgtcgacga agagatcgtt catcagcacc
     4141 acctcgatcg cccgggtcga gctgaagtgc cagtagaggc aggcgtagcc gaggaagccg
     4201 ccctcgccgc gggcgccgag cagcagcccg tcgtcgctgg gggcgaggaa gcggcggaag
     4261 aactcgcggt tgcggtcggt gtcggggttc tcgaccccgt agaagcgctg gtagttcgcg
     4321 atcagcggca acagctcctc gaactgcgcg gggctgaccg gggagatctc cacgaatcca
     4381 ccgtatcggg cgcaagccgc cgaccacggg gagggacggc ggggcaggcg ccgtaaacga
     4441 cccccacgca acccggcagg caggcccgtc taagctttcg gggccgccgg aggtctagat
     4501 agatcccgtt aggaggggat tccgctggca cgtcccaccg attcgaacga gcaactcctc
     4561 tgcagcttct gcggcaagtc gcagcgccag gtcaaaaagc tgatcgctgg cccgggcgtc
     4621 tacatctgcg atgagtgcat cgacctctgc aacgagatca tcgacgagga gctgaccgc
//
LOCUS       DASRIG010000397        12573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1241679, whole genome shotgun sequence.
ACCESSION   DASRIG010000397 DASRIG010000000
VERSION     DASRIG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241679"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFB52_10625"
     CDS             complement(<1..404)
                     /locus_tag="VFB52_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZO06832.1"
                     /translation="MAVEIRILGRVDALVDGRVLPLRGSKQRAVLAMLALRANRTVSA
                     DSLIDGLWGERPPESAAKNVQTHVSRLRKALAADGSEASIVTHGRGYELQLSEEAVDA
                     VRFERLVERARRDADQGIADGAAKQALELWHGA"
     gene            complement(466..1419)
                     /locus_tag="VFB52_10630"
     CDS             complement(466..1419)
                     /locus_tag="VFB52_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06833.1"
                     /translation="MPVARPLEVEVRPPWPYRLPSRLGGDGTSRRRGGVLSRLLDVGG
                     ERVVVHAWQPERIRVVLRAAGCAPPTTPAALELAVERMRFALGVDEDLSEFARAFRGD
                     PLIGEAIHHRPWHRPRRRPWPWEALAWAVTEQLIEARRAAQIQRWIVRRWGGKLLPPD
                     PSNGGGGPPPWLGPGPLRDVPTAAVVAGVAPAELAAYDLAPSRAIALIHCAREVAAGR
                     VDPADAAGDRRLRRIPGIGPWTLQVLGLGGRGEPDSLPAGDLAYVKLVGVLNRLGRRA
                     TVAEVEEYFAPYAPFRGLAGSFALVRWHGAVGQAPPLRRAA"
     gene            complement(1424..1747)
                     /locus_tag="VFB52_10635"
     CDS             complement(1424..1747)
                     /locus_tag="VFB52_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06834.1"
                     /translation="MREELLETEAIEMPANDDGAGDESVARPLPARREAGTDLSIWRD
                     EVRTAALAAAGGLVAGAATVAVVRASRGSSGRRAAKRSRGRERPVKVVASRSFLVDVH
                     LLGEK"
     gene            complement(1770..2924)
                     /locus_tag="VFB52_10640"
     CDS             complement(1770..2924)
                     /locus_tag="VFB52_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06835.1"
                     /translation="MADLSIRPVRTRRERNRFIEVPYRLRRDDPQWVPPLRFERRQFL
                     DPDKNPWFEHAEAQLFVAERGGEDVGRVSAHIDHRWDEFQGGSDGMFGFFETADDPEV
                     FAALIGAAGDWLRERGRERMLGPMDFTLNDECGILIEGYDEPSMILEPWHPPYYARRL
                     EELGLTKQIDLLMWELWFGRLKAGTELHPLIHAAAEKSRDEGVVLRSMRKRDMAAEVA
                     RFMEVYNEAWGDNWGFVPITEAEVEFQAKNLKPVLDENWAMIAEKDGEVVGAALTLPD
                     VEQALGRMNGRVLPFGWWHFLRRRSYIDRLRVFALGVKHDYQHLGVAAALYERHVQTA
                     AAHGPRGGHMGWILETNDPMNRAMEGMGGRIVQKYRIYRKPLAERGTAAA"
     gene            2950..4212
                     /locus_tag="VFB52_10645"
     CDS             2950..4212
                     /locus_tag="VFB52_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO06836.1"
                     /translation="MAETTRAPVDVFEKARTHDRSEQLEMAKEADLLPYFRVLDSEAG
                     PVVEMEGRETIMLGSNNYLGLTGDPRVKQAARDALETFGTGLTGSRLLNGTTPLHLEL
                     ERELAEWMQTEDVIVFSTGYQTNVGCIGTVLAPGDTVICDSADHASILDGCKLSGAKL
                     RPFRHNQMPKLEKMLDRAAGDGGGVMVVVDGVYSMEGDLPPLPAIAELCGRYGARLMV
                     DEAHGVGVLGARGAGATELFGLEDRVDLRMGTFSKSLASCGGFIAGPADVVEYLRIAS
                     RSFIFSASAVPAAVGAALGALRVIRSEGPELMAKLLDNAAYLRRGFRDLGLRVVEPGT
                     LPDGREATTPVVPVIVGEDWQAVLLWKALFERGVYTNVAIHPAVPPGGALLRTSLMAS
                     HEREHLDRALALVEATIADFPDLPRSSS"
     gene            4534..4878
                     /locus_tag="VFB52_10650"
     CDS             4534..4878
                     /locus_tag="VFB52_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06837.1"
                     /translation="MEAASSLAPVAIPQHMQALEHANRVRLARASLKRSVAAGRADAA
                     EIVRECPWEVQSMSIGELLRSQRRWGRTRARKFLFPMALNENRELGRLTTRQRELLAT
                     ELEAKRPERTMA"
     gene            complement(4953..5342)
                     /locus_tag="VFB52_10655"
     CDS             complement(4953..5342)
                     /locus_tag="VFB52_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06838.1"
                     /translation="MILSRVREIMSGPASSALVLLMLAGCLFLWIGVPLGWLWIGSQV
                     QGSASLGTAMLVTMLGITATIFVVVIVLSWLNRRHAVLQERRNRPRGGNALEMLLVAS
                     AGIAIVGFGVWFFGFAGASPVPLNIGF"
     gene            complement(5365..6390)
                     /locus_tag="VFB52_10660"
     CDS             complement(5365..6390)
                     /locus_tag="VFB52_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06839.1"
                     /translation="MPSALDQITAPTAPGPETLGGTVPAGAERRARDDLRRQIADLDR
                     RLGELFASAFPRSGFDWGVGAVGGPRVLSTGELERVRDALALRLRDAQAELARRAEVE
                     EANRGLIERMIAAPERHRWVIVSNEDIGERGCRHWHSRPRWGILGILFNWWRVRLSSG
                     CPLAAGLRPPATRVTAPSEMAKKRRKRRPRAPAAAPAAAPKPAAKRPAAKVPRKPIAD
                     ERPAAPWGSFPLVELVVLFGLVLFVVGFFLVDGERGTQLFATGLVIASLAGLELSIRE
                     HFAGYRSHSTLLAAAVAVAVMLGLYYLADLSPTASLAAGAGVGALCFWALARTFQNRA
                     GRMVKLR"
     gene            complement(6461..7123)
                     /locus_tag="VFB52_10665"
     CDS             complement(6461..7123)
                     /locus_tag="VFB52_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HZO06840.1"
                     /translation="MSAGAEASGETPPQLGWVAPELVEEFPGLALHYLAVERGSGRST
                     RELRQQLRVLSDRFSGAQAINLRHQPIPWAYRVFYRHIGLDPDQQRTPIEELVLERMQ
                     HGGFRSRSLLDDALTIATVESSVAMRAFDADRVTGRPGIRTTEPGEGLEGRPGALPAG
                     TLVIADEARPLALLFGALAAGRGVRPKTKRTLLAAIQVTGVPALAVSEALWLAAGTLE
                     DG"
     gene            complement(7120..7827)
                     /locus_tag="VFB52_10670"
     CDS             complement(7120..7827)
                     /locus_tag="VFB52_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06841.1"
                     /translation="MTGDTDGSSSKRRPSSRYSAFVGLAFIVLIVIAAINTFESNEGG
                     VLGADETQVGEPLPEFAVPELIGGEDADANVYQDDCESSEVPCPADEQRTSACDVDVP
                     NVIRICDLFDRPLVLSFWFSTPAECPPTQDVVDNAARRYGGRVNFLSLAVRGDREELA
                     DIVAERGWELPVGWDRDGAVSNLYRVGVCPTVAFVLPGGILSEARVGSDELDQRQLDV
                     AIERLIRESQRDAEAPQ"
     gene            complement(7858..10557)
                     /locus_tag="VFB52_10675"
     CDS             complement(7858..10557)
                     /locus_tag="VFB52_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO06842.1"
                     /translation="MRDLLARIAGFCVERPLPVLAATALVTLVAAVGALGLEADAGTD
                     QLVDRDSDAFVGTEAFKREFGDDAVVVLVKGDLEQLVLSSDLGKLLALEACLSGKAPG
                     GRVFPDEPAPAPCAALAESRAARVVFGGATFLNQSAIQAERLLAQQSSAAQQQARAAG
                     ARAALDARRQGLSESDQRAAAQAAAQEVLTTFQQQVLDLAIRYGQTGIPRLDDPTFVQ
                     SVVFDRRSPGEPKPRFSAFWPSADAAQILIRLRPELSETERREAIEQIRAAVDDSTFR
                     IRDAEYVVSGVPVVVEGLAQKLSDEIFVLLTVALAVMALTLALLFGPPMRLLPLGIAM
                     VAAAAVFGLLAAFGGSLTMASLAVLPILIGLAVDYAIQFHARFAEAARAGASAPRAAV
                     EAAAVGGPVIGAAALATAAGFLVLLLSPIPMIRGFGLLLVAGIAIAFVLALTAGLATL
                     SLTRPAAGRTPPSGRLPAPLIAALERIGQARRAISERAGSLGRRALAASVAAPGRVLA
                     VGLIVAVIGWALGTRTEVISDIRELVPRNLPELQNVDELQEATGVSGEVDVTVNAPDL
                     TEPEVIAWMSDYKQRVLSRAGFTSEATSCREQDTQLCPSIALPDLFGGEGEAPDQARI
                     RRTLALLPPYFSQAVINSDPDTGELGDTAVIAFGIKVMPFDEQKQLIDAIRAEIDPPG
                     TGNDPPDGVSAEVVGLPVLAADANSALEGNRYLLTFAGLAAVALALLAVYRSLRRALV
                     PLIPVALATGWSALGLAITGVPLNPMSATLGALVIAIATEFSVLIAARYEEERRTALS
                     VGEALRRAYSRTGVAVIASGITAIAGFATLIATDIRMLRDFGIVTVIDLSVALVGVLI
                     ILPAALVWAESGFRTTPFRTTPTASSASRPVSSGGDALREGRP"
     gene            complement(10624..11640)
                     /locus_tag="VFB52_10680"
     CDS             complement(10624..11640)
                     /locus_tag="VFB52_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06843.1"
                     /translation="MAEERGYPDERGSDEQVAEEQPADEQVAQEQEYDRFADDVGRDD
                     RGEDYDEPAPELGDLHRDYSIPGVDSKESNPYRWHTGEKRDHGGEDAIEAIDLVKQFG
                     RTRILNGLNLGLPDNQISMVLGPSGTGKSVLIKHIVGLLYPDTGDLLVHGESVPNMTD
                     DELFDMRKKFGLLFQDGALFGSMNVYDNTAFPLRQHTDKGEDEIGEIVNRRLREVGLA
                     EATEKMPNELSGGMRKRAGFARALVLDPAIVMFDEPDSGLDPVRTALLCELIREVHEE
                     NGGCYLVISHDLGTARRIADFIAVLWKGRIVESGPSEELFNSENEFVSQFLNAQTEGP
                     LAMD"
     gene            complement(11677..12477)
                     /locus_tag="VFB52_10685"
     CDS             complement(11677..12477)
                     /locus_tag="VFB52_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06844.1"
                     /translation="MLMGWLAVPRDWLASLGDIARFCGRVMGIVYSGRVMHFFGEALR
                     QAGILILGSTVVIWGLVFILGLQCGIEGAYFNRSVGAPAYAGVFAAWCDLREVIPYAF
                     GYMMAAKIGTGIVAELGAMRISDEIDALEVMGVNPMIFLCGTRLLGAWMVLPFMYLAA
                     IGIGYLASYLAVVEQIGEVSSGGYFLIFWMFQNPPDLIFSLIKAMFMATVIVLVGCYY
                     GYTASGGPVGVGTATAKSMVLNIVLVHIIGMMGTLVFWGANPRAPIGG"
BASE COUNT         1946 a         4519 c         4410 g         1698 t
ORIGIN      
        1 gccccgtgcc agagctcgag cgcctgcttg gcggcgccgt cggcgatccc ctgatccgca
       61 tcgcgccgag cacgctcgac caggcgctcg aaccgcaccg catccacggc ctcctcggag
      121 agctgtagct cgtagccgcg accgtgggtg acgatgctcg cctcggagcc gtcggcggcg
      181 agtgcctttc gcaggcgcga gacgtgggtc tggacgttct tggcggcgct ctcgggcggg
      241 cgctcacccc agaggccatc gatcagcgag tccgcgctga ccgtgcggtt ggcacgcagc
      301 gcgagcatcg cgagcaccgc tcgctgcttg cttcctctga gtggcaggac ccggccgtcg
      361 acgagcgcat cgacacggcc caggatgcgt atctcgacag ccacgtaccc cccctttcgg
      421 gaagaccgta cacccggcgg ctgctgcgat caactcgcgt gacgctcagg cggcgcgccg
      481 cagcggcggc gcctgcccga ccgccccgtg ccagcggacg agcgcgaagc tgccggcgag
      541 gccccggaac ggcgcgtagg gggcgaagta ctcctcgacc tcggcgaccg tcgcccggcg
      601 cccgagccgg ttgagcacgc cgacgagctt gacgtatgcg agatctcccg ccggcagcga
      661 gtcgggctcg ccgcggccgc cgagcccgag cacctgcagg gtccacgggc cgatcccggg
      721 gatccgtcgg agtcgccggt ccccggccgc gtcggcggga tcgacgcgtc cggccgcgac
      781 ctctcgagcg cagtggatga gggcgatcgc gcgcgacggc gcgaggtcgt aggcggcgag
      841 ctcggcgggc gcgacgccgg cgaccactgc ggcggtcggc acgtcccgga gcgggccagg
      901 cccgagccag ggtggcggtc ctccgccgcc gttcgacggg tcgggcggca ggagctttcc
      961 gccccatcgc ctcacgatcc agcgctgaat ctgtgccgcg cggcgcgcct cgatcagctg
     1021 ctcggtgacg gcccaggcga gcgcctccca cggccacggg cgccgcctcg gacggtgcca
     1081 ggggcgatgg tggatcgcct ccccgatcag cggatcgccg cggaaggcgc gggcgaactc
     1141 gctcagatcc tcgtccacgc cgagcgcgaa ccgcatccgc tcgacggcga gctcgagcgc
     1201 tgcgggggtt gtcggcggcg cgcatcccgc ggcgcgcagg accacgcgaa tgcgctcggg
     1261 ctgccaagcg tggacgacga cacgctcgcc cccgacgtcc agcagccgcg agagcacgcc
     1321 gccccggcgc cgggaggtgc cgtccccgcc cagtcgcgac gggagccggt acggccacgg
     1381 cggccggacc tcgacctcca gcggccgtgc gacgggcacg gagctacttt tcgccgagca
     1441 ggtgcacgtc caccaggaac gagcggctgg cgacgacctt caccgggcgc tcgcggccgc
     1501 gcgagcgctt cgccgcgcgc cgtccggagc tgccgcgcga cgcgcgcaca accgcgaccg
     1561 tagcggcccc ggccaccagc ccgccggccg ccgcaagcgc ggccgtccgg acctcgtcgc
     1621 gccagatcga gaggtcggtc cccgcctcgc ggcgggcggg cagcggccga gcgacggact
     1681 catcaccggc gccgtcgtcg ttcgccggca tctcgatcgc ctcggtctcc agcagctcct
     1741 ctcgcacggg ctcctactct aagggcttgt caagcggcag cggtcccgcg ctcggcgagc
     1801 ggcttccggt agatgcggta cttctggacg atccggccgc ccatcccctc catggcgcgg
     1861 ttcatcgggt cgttcgtctc caggatccag cccatgtgac cgccgcgcgg cccgtgggcc
     1921 gccgccgtct gcacgtggcg ctcatagagc gccgcggcga cgccgaggtg ctggtagtcg
     1981 tgcttgacgc cgagcgcgaa cacgcgcagg cggtcgatgt aggaacggcg gcgcaggaaa
     2041 tgccaccagc cgaacggcag caccctgcca ttcatccgcc ccagcgcctg ctcgacgtcg
     2101 ggcagcgtca gcgccgcgcc caccacctcg ccgtccttct cggcgatcat cgcccagttc
     2161 tcgtcgagga ccggcttcag gttcttggcc tggaactcga cctcggcctc ggtgatcggg
     2221 acgaaccccc agttgtcgcc ccatgcctcg ttgtagacct ccatgaagcg cgcgacctcg
     2281 gccgccatgt cgcgttttcg catcgagcgc aggacgacgc cctcgtcgcg agacttctcg
     2341 gcggccgcgt ggatcagcgg gtgcagctcg gtgcccgcct tgaggcggcc gaaccacagc
     2401 tcccacatca gcaggtcgat ctgcttggtg agcccgagct cctccaggcg ccgcgcgtag
     2461 tacggcggat gccacggctc gaggatcatc gagggctcgt cgtagccctc gatcaggatc
     2521 ccgcactcgt cgttgagcgt gaagtccatc ggtccgagca tccgctcgcg gccgcgctcg
     2581 cgcagccagt cgcccgcggc cccgatcagg gccgcgaaga cctcgggatc gtcggcggtc
     2641 tcgaagaagc cgaacatgcc gtcgctgccg ccctggaact cgtcccaccg gtgatcgatg
     2701 tgggcgctga cgcgaccgac gtcctccccg ccccgctcgg cgacgaagag ctgcgcctcg
     2761 gcgtgctcga accacgggtt cttgtcgggg tcgaggaact gccggcgctc gaagcgaagc
     2821 ggaggcaccc attgcgggtc gtcgcgccgc agccgatagg ggacctcgat gaagcggttg
     2881 cgctcgcgcc gcgtgcgcac cgggcgtatc gacaagtccg ccacggctgc ggaacctaca
     2941 cttcgccaca tggccgagac gacaagggcc ccagtcgacg tgttcgagaa ggcccggacc
     3001 cacgaccgct cggagcagct cgagatggcc aaggaggccg acctgctgcc ctacttccgg
     3061 gtcctcgact ccgaggccgg gcccgtggtc gagatggagg ggcgcgagac gatcatgctc
     3121 ggctcgaaca actacctcgg attgaccggc gacccgcgcg tcaagcaggc ggcccgcgac
     3181 gcgctcgaga ccttcgggac gggcctcacc gggtcccggc tgctcaacgg caccaccccg
     3241 ctgcacctcg agctcgagcg cgagctcgcc gagtggatgc agaccgagga cgtcatcgtc
     3301 ttctcgaccg gctaccagac gaacgtcggc tgcatcggca cggtcctcgc cccgggcgac
     3361 acggtgatct gcgactcggc cgaccacgcc tcgatcctcg acggctgcaa gctctcgggc
     3421 gccaagctcc gccccttccg ccacaaccag atgccgaagc tcgagaagat gctcgaccgg
     3481 gcggccggcg acgggggcgg cgtgatggtg gtggtcgacg gggtctactc gatggagggc
     3541 gacctgccgc cgctgccggc gatcgccgag ctctgcggcc gctacggcgc gcggctgatg
     3601 gtcgacgagg cgcacggggt cggcgtgctg ggcgctcgcg gagccggcgc caccgagctc
     3661 ttcggcctcg aggaccgggt cgatctgcga atgggcacct tctccaagag cctcgcctcc
     3721 tgcggcggtt tcatcgccgg gcccgccgac gtcgtcgagt acctgcggat cgcgagccgc
     3781 tcgttcatct tcagtgcctc ggccgtcccc gcggccgtcg gcgcggcgct cggtgcgctg
     3841 cgggtgatcc ggtcggaggg gcccgagctg atggcgaaac tgctcgacaa cgccgcctac
     3901 ctgcgacggg gcttccgcga tctcggcctg cgggtcgtcg agccgggcac gctgcccgac
     3961 ggccgcgagg caaccacccc ggtggtcccg gtgatcgtcg gcgaggactg gcaggcggtg
     4021 ctgctctgga aggcgctctt cgagcgcggc gtgtacacca acgtcgcgat ccaccccgcc
     4081 gtgccgccgg gaggcgcgct gcttcgcacg tcgctgatgg cgagccacga gcgcgagcac
     4141 ctggatcggg cgctggcgct ggtcgaggcg acgatcgcgg acttccccga cctcccgcga
     4201 tcgagctcct gaggaggccg cggcggagcc gccccgttcc ccccagtggg ggactttcac
     4261 gttgtccact tctggacaag acaagcccac gcacaaaaag catgctctta gcgaacgagt
     4321 gtgggggcac gccggaccgg cccggttgac gcgggcgctc cgggttccat aggttgcgaa
     4381 ggcgccgaac gcaccctggg gcagggagcg gtcgggacag aaaagagtga tttgtcccga
     4441 cccaccctgg agcggcaacg ggccgcgatt gggagcaccg ggcgccgata tacccccgca
     4501 acccccaaac cgctcacgag gaggagagtg agaatggaag ctgcctcgag cctggctcct
     4561 gtagcgatcc cgcagcacat gcaggcgctc gaacacgcca accgcgtccg gttggcgagg
     4621 gcgtcgctca agcgctcggt cgccgcaggt cgggccgatg cggcggagat cgtccgtgag
     4681 tgtccctggg aagtccagtc aatgtcgatc ggcgagcttt tgaggagcca gcggcggtgg
     4741 ggccgtacgc gggctcgtaa gttcctgttc ccgatggcgt tgaacgaaaa tcgtgagctt
     4801 gggcggctga ccacccgtca gcgtgagctc ctggcaacgg agctcgaggc caagcggccc
     4861 gaacgcacga tggcgtaaga ctcgacggga tcgtcccagc ggtcgaaggg gcgggcgcgc
     4921 gacgcccgcc ccgccgccga ccccggaacg cctcagaagc cgatgttgag cggcaccggg
     4981 ctggcaccgg cgaagccgaa gaaccagacc ccgaatccca cgatcgcgat gccggcgctg
     5041 gcgaccagga gcatctcgag cgcattaccg ccacgggggc gattgcgacg ctcctggagc
     5101 accgcgtggc gacggttgag ccacgagagc acgatcacca cgacgaagat cgtcgccgtg
     5161 atcccgagca tggtcacgag catcgcggtg cccagcgacg ccgacccctg aacctgcgat
     5221 ccgatccaga gccagccgag cgggacgccg atccacagga acaggcaccc ggccagcatg
     5281 agcaacacca gcgcgctgct ggcgggacca ctcatgatct cgcgcacgcg ggagaggatc
     5341 accggcgagc gtaccggcca tcgctcagcg cagcttgacc atacgcccgg cgcggttctg
     5401 gaacgtccgt gccagcgccc agaagcagag cgcgccgacc cccgcgcccg cggcgaggct
     5461 ggcggtcggc gacaggtccg cgaggtagta gaggccgagc atcacggcta ctgcgacggc
     5521 ggcagcgagg agggtcgagt gcgagcgata gcccgcgaag tgctcgcgga tcgacagctc
     5581 cagcccggcg agcgacgcga tcacgaggcc ggtcgcgaac agctgcgtcc cgcgctcgcc
     5641 gtcgaccagg aagaagccga ccacgaacag cacgaggccg aagaggacca cgagctcgac
     5701 cagggggaac gagccccacg gcgcagccgg gcgctcatcc gcgatcggct tgcgggggac
     5761 cttcgcagcg ggacgcttgg cggccggctt gggcgcggcg gccggcgcgg ccgcaggggc
     5821 gcggggtcgg cgcttgcggc gtttcttcgc catctcagat ggtgccgtga cccgcgtggc
     5881 cgggggccga agccccgcgg ctaacggaca accggacgag aggcgaaccc gccaccagtt
     5941 gaacaggatc cccaggatgc cccagcgcgg gcgggagtgc cagtggcgac agccccgctc
     6001 gccgatgtcc tcgttcgaca cgatcaccca gcgatggcgc tccggcgcag cgatcatgcg
     6061 ctcgatcagg ccccggttcg cctcctcgac ctcggcgcgc cgggcaagct ccgcctgggc
     6121 gtcgcgcagg cgcagggcga gggcgtcgcg gacccgctcg agctcgccgg tcgagagcac
     6181 ccgcgggccg ccgaccgcgc cgacccccca atcgaacccg gaacggggga aggcggaggc
     6241 gaagagctcg ccgagtcggc ggtcgaggtc cgctatctgg cgccggaggt cgtcacgggc
     6301 gcgccgctcg gcgccggcgg ggacggttcc gccgagggtc tcggggccgg gcgctgtggg
     6361 ggccgtgatc tggtcgagcg cgctgggcac ggaggctcct ttacccgttg cctgtccgcc
     6421 gcattctaat cggccaccgg ccgcgcttcc agcgaggggc ctagccgtcc tcgagagtgc
     6481 cggcggcaag ccagagcgcc tcggagaccg cgagcgccgg cacgccggtc acctgaatcg
     6541 cggcgagcag cgtgcgcttc gtcttcggtc ggacgccgcg gccggccgcg agcgccccga
     6601 agagcagcgc gagcggacgg gcctcgtcgg cgatcacgag cgtgccggcc ggaagcgcgc
     6661 cgggtcgccc ctccagcccc tcccccggct cggtcgtgcg gatccccggc ctgccggtga
     6721 cccggtccgc atcgaaggcg cgcatcgcaa cgctcgactc gaccgtggcg atcgtgagcg
     6781 cgtcgtcgag caggctgcgg ctgcgaaagc cgccgtgctg catccgctcg agcaccagct
     6841 cctcgatcgg cgttcgctgc tggtcggggt ccaggccgat gtggcggtag aagacgcggt
     6901 acgcccaggg gatcggctgg tggcggagat tgatcgcctg ggctcccgag aaccggtcgg
     6961 acaggacgcg aagttgctgt ctgagctcgc gggtcgagcg ccccgagcct cgctcgaccg
     7021 cgaggtagtg aagggccagc cccgggaact cctcgacgag ctccggcgca acccagccga
     7081 gctgcggcgg cgtctccccg gacgcctcgg ctcccgcgct cattgcggcg cctccgcgtc
     7141 gcgctgggac tcccggatca ggcgctcgat cgcgacgtcc agctggcgct ggtcgagctc
     7201 gtcgctgccg acgcgggcct cgctcaggat cccacccggg agcacgaacg ccaccgtcgg
     7261 gcagactccg acccggtaga gattcgagac ggccccgtcg cgatcccagc cgacggggag
     7321 ctcccagccg cgctcggcga cgatgtcggc gagctcctcg cggtcgccgc ggacggcgag
     7381 cgagaggaag ttgacccgcc cgccgtaacg ccgcgcggcg ttgtcgacga cgtcctgggt
     7441 cggcgggcac tcggccggcg tcgagaacca gaacgacagc accagcggcc gatcgaagag
     7501 atcgcagatc cggatcacgt tgggcacgtc gacgtcgcac gccgaggtgc gctgctcgtc
     7561 ggcgggacag ggaacctccg agctctcgca gtcgtcctgg tagacgttgg cgtcggcgtc
     7621 ctcgccgccg atcagctcgg gcaccgcgaa ctccggaagg ggctcgccga cctgcgtctc
     7681 gtcggcgccg agcactccgc cctcgttgct ctcgaacgtg ttgatggcgg cgatcacgat
     7741 caggacgatg aacgcgaggc cgacgaaggc cgagtagcgg ctcgacggcc gccgcttgga
     7801 tgacgagccg tcggtgtcgc cggtcacggg ctagcgacga agtcgcggtt caagcgctta
     7861 tggcctgcct tcccgtaacg cgtcgccgcc cgacgacacc ggacgcgaag cggaggatgc
     7921 cgtcggagtt gttcggaacg gagttgttcg gaagcccgat tcggcccaga ccagcgccgc
     7981 cgggaggatg atcagcacgc cgaccagggc gaccgagagg tcgatcacgg tgacgatgcc
     8041 gaagtcgcgc agcatgcgga tgtccgtcgc gatcagcgtc gcgaacccgg cgatcgcggt
     8101 gatccccgag gcgatcacgg cgacccccgt gcgcgaatag gcccgccgca gggcctcgcc
     8161 gacggagagc gccgtgcgcc gctcctcctc atagcgcgcg gcgatcagca cgctgaactc
     8221 ggtcgcgatc gcgatgacca gcgcgccaag cgtcgccgac atcgggttca ggggcacgcc
     8281 ggtgatcgcc agcccgagcg ccgaccagcc ggtggcgagc gcgaccggga tgagcggcac
     8341 cagcgcgcgc cgcagcgacc gatagacggc cagcagggcg agcgccaccg ccgccagccc
     8401 ggcgaaggtc agcaggtagc ggttgccctc gagcgccgag ttcgcatctg ctgcgagcac
     8461 cggcaggccg accacctcgg cgctgacacc gtcgggcggg tcgttccccg tgcccggcgg
     8521 gtcgatctca gccctgatcg cgtcgatcag ctgcttctgc tcgtcgaacg gcatgacctt
     8581 gatcccgaac gcgatcaccg cggtgtcgcc gagctcgccc gtgtcggggt cgctgttgat
     8641 cacggcctgc gagaagtacg gcggcaggag cgcgagcgtc cgccgaatcc gtgcctggtc
     8701 cggcgcctca ccctcgccgc cgaacaggtc cggaagggcg atcgacgggc acagctgcgt
     8761 gtcctgctcg cggcaggacg tggcctcgct ggtaaagccg gcgcgggaga ggacgcgctg
     8821 cttgtagtcg ctcatccacg cgatcacctc gggctccgtg aggtccggcg cattcaccgt
     8881 gacgtcaacc tcgccggaga ccccggtcgc ctcctggagc tcgtcgacgt tctggagctc
     8941 gggcaggttg cgggggacga gctcacggat gtcggagatc acctccgtcc gggttcccag
     9001 cgcccaaccg atcaccgcga cgatgaggcc cacggccagc actcggccgg gcgccgcgac
     9061 cgatgcggcc agggcccgcc ggccgagcga accggcccgc tcggagatcg cccggcgagc
     9121 ttggccgatg cgctcgagcg cggcgatcag cggcgccggg aggcggccac tcggcggcgt
     9181 gcggccggct gccggcctcg tgagcgacag cgtggccagc ccggcggtca gcgcgagcac
     9241 gaacgcgatc gcgatcccgg ccaccaacag caggccgaac ccgcgaatca tcgggatcgg
     9301 cgacagcagc aggaccaaga agccggcggc cgtggccagc gccgccgccc cgatgacggg
     9361 cccacccacc gccgctgcct cgaccgcggc ccgaggggcg gaggcgcctg cacgtgccgc
     9421 ctccgcgaac cgggcatgga actggatcgc gtaatcgacc gcgagcccga tcaggatcgg
     9481 cagtaccgcc agcgacgcca tggtgagtga gccgccgaag gcagcgagca gtccgaacac
     9541 cgcggccgcg gcgaccatcg cgatcccgag cggcagcagc cgcatcggcg gaccgaagag
     9601 cagcgccagg gtcagggcca tcaccgcgag cgccaccgtc agaaggacga agatctcgtc
     9661 cgagagcttc tgggcgaggc cctcgacgac gaccgggacg ccgctgacga cgtactcggc
     9721 gtcacggatg cggaaggtcg agtcgtcgac cgcggcgcgg atctgctcga tcgcctcgcg
     9781 gcgctcggtc tcgctgagct cggggcgcag ccggatcagg atctgggccg catcggcgct
     9841 cggccagaac gcggagaacc gcggcttcgg ctcgcccggg ctccgcctgt cgaagaccac
     9901 ggactggacg aaggtcggat cgtcgagccg cgggataccg gtctgcccgt agcggatcgc
     9961 gaggtcgagt acctgctgct ggaaggtggt cagcacctcc tgcgcggctg cctgcgccgc
    10021 ggcccgctgg tcgctctccg acagtccctg gcggcgggca tcaagcgccg cgcgcgcccc
    10081 ggccgcgcgc gcctgctgct gcgccgcgct cgactgctgc gcgagcagcc gttccgcctg
    10141 gatcgccgac tggttgagga aggtggcccc accgaagacc acccgcgccg cccggctctc
    10201 ggccagcgca gcgcacggcg ctggggccgg ctcgtcgggg aacaccctgc ctccgggggc
    10261 cttgccggac aggcacgcct cgagcgccag cagcttgccg aggtccgagc tgaggacgag
    10321 ctgctccagg tcgcccttga ccaggacgac gaccgcgtcg tcgccgaact cccgcttgaa
    10381 cgcctcggtc ccgacgaagg cgtcggagtc acgatcgacc agctggtccg tgccggcgtc
    10441 ggcctcgagc ccgagcgcgc cgacggcggc gaccagcgtc accagcgccg tcgcggccag
    10501 cacgggcaga ggccgctcca cacagaagcc cgcgatccgt gcgagcagat ccctcatcga
    10561 ctcaagggta gggacgtgcg cccgcgatca agccgacgga aaccgagcgc ggcgcgagcc
    10621 gcctcaatcc attgcgagcg ggccctccgt ttgggcgttc aggaactggg agacgaactc
    10681 gttctcggag ttgaagagct cctcggacgg gcccgattcg acgatccggc ccttccagag
    10741 gaccgcgatg aagtcggcga tccgccgcgc ggtgccgagg tcgtggctga tcaccaagta
    10801 gcagccgccg ttctcctcgt gaacctcgcg gatcagttcg cagagcagcg ccgtgcgcac
    10861 cgggtcgagg cccgagtcgg gctcgtcgaa catgacgatc gccgggtcga gcaccagggc
    10921 gcgggcgaat ccggcgcgct tgcgcatccc gccggacaac tcgttcggca tcttctcggt
    10981 cgcctcggcg aggccgacct cgcgcaggcg ccggttgacg atctcaccga tctcgtcctc
    11041 gcccttgtcg gtgtgctggc gcagcgggaa ggccgtgttg tcgtacacgt tcatcgagcc
    11101 gaacagggcg ccgtcctgaa acaacaggcc gaacttcttg cgcatatcga acagctcgtc
    11161 atcggtcatg ttgggcaccg actcgccgtg gacgagaagg tcgcccgtgt cgggatagag
    11221 caggccgacg atgtgcttga tcaacacgct cttgccggtg cccgacgggc cgagcaccat
    11281 cgagatctgg ttgtcgggca ggccgaggtt gaggccgttg aggatccggg tgcgcccgaa
    11341 ctgcttcacc aggtcgatgg cctcgatcgc gtcctcaccg ccgtggtcgc gcttctcgcc
    11401 cgtgtgccag cgataggggt tcgactcctt cgagtcgacg ccggggatcg agtagtccct
    11461 gtgcaggtct ccgagctcgg gtgccggctc gtcatagtcc tcgcctcggt cgtcgcggcc
    11521 gacgtcgtcg gcaaagcggt cgtactcctg ctcctgggcg acctgctcgt cggcgggctg
    11581 ctcctcggcg acctgctcgt cgctgccacg ctcgtcggga tacccgcgtt cctcagccac
    11641 tctgcctact cctcctgatg gtcggtcatt gtctgctcag ccgccgatcg gcgccctcgg
    11701 gttcgctccc cagaagacca gcgtccccat catcccgatg atgtggacca gcacgatgtt
    11761 gagcaccatc gacttcgcgg tcgcggtgcc gacgccgacg gggccgccgg aggccgtgta
    11821 gccgtagtag cagccgacga gcacgatcac cgtcgccatg aacatcgcct tgatcaagct
    11881 gaatatgaga tccggcgggt tctggaacat ccagaagatc aggaagtagc cgccggacga
    11941 aacctcgccg atctgctcga ccaccgccag gtaggaggcg aggtagccga tcccgatggc
    12001 ggccagatac atgaacggca gcaccatcca ggcgcccagc agccgcgtgc cgcagaggaa
    12061 gatcatcggg ttgacgccca tcacctcgag cgcgtcgatc tcgtcggaga tccgcatcgc
    12121 gccgagctcg gcgacgatcc cggtaccgat cttcgccgcc atcatgtagc cgaaggcgta
    12181 ggggatcacc tcgcgcaggt cgcaccaggc cgcgaacacg ccggcgtagg ccggcgcgcc
    12241 gaccgagcga ttgaagtacg cgccctcgat cccgcactgc aggccgagga tgaagacgag
    12301 cccccagatc acgacggtcg agccgaggat caggatcccc gcctggcgca gcgcctcccc
    12361 gaagaagtgc atcacccggc cggagtagac gatccccatc acccggccgc agaagcgggc
    12421 gatgtcgccg agcgacgcga gccagtcgcg cggtaccgcc agccagccca tcagcatgcc
    12481 tccaagtccg cagtcagcag cctgcagtcc gcagatcttg cggccggtcc ctgcgggctg
    12541 cggactgcgg actgcggact gatcacttga tca
//
LOCUS       DASRIG010000398         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1241897, whole genome shotgun sequence.
ACCESSION   DASRIG010000398 DASRIG010000000
VERSION     DASRIG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241897"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VFB52_10690"
     CDS             complement(<1..1646)
                     /locus_tag="VFB52_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06845.1"
                     /translation="MAGTGKYWWPPEPGPEQTVREMLGKRVAERGRKPALIAPSLLDA
                     GAETTLTYGQLGERAARMASVLAAAGVGPGDRVGILLDNDGAVEAHVTYHASHLLGAI
                     NVPLNTRYVQRELEYVLGFIEPAALVFAPAFAERLGTLRPALGDAALIEATTGPGALG
                     RPLAELLASVEPLAEPTPLDPDQDADWLFTSGTTGNPKAVALTHRGSVACGYQSVGAW
                     ALEPNAVYQSFAPFFTSTGCHSNLLACVAAGCAYVVEPEFDVHATLERIERHRTTSTF
                     LINTVLALILERRTPEELDAYDFSALRRVCYGAQPSSPAFYRRVWEEIGRKWDVELVN
                     VYGLTEGGTTGIYLSDADHPEALERIGPWGLSIGRTGFREWVEWTVLREEDDAPAAPN
                     EVGELCVRGPSTMSRYVDNPEETARALRDGWLHTGDSATLDEDGFLFFVDRNKQLIRR
                     GGLNISSAEVEGVLLEHPAVFEVAVVPLPNPVFGHDVRGVVVPEPGTSPTPEELIAFC
                     AERLADYKVPTQIDFIDALPRNGMNRVIKGVLTGDNDSLSA"
     gene            complement(1651..>2252)
                     /locus_tag="VFB52_10695"
     CDS             complement(1651..>2252)
                     /locus_tag="VFB52_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06846.1"
                     /translation="PVHGGEPTAPEHERLMYYGPQFSRGHFNQHTGVRGSIRVGDEEF
                     PLPDAYGWRDHSWGPRYWQVIWAYRLLIANFGPNRAFMLLKNIMPDGSSRRLGVLQID
                     GEYHEVTDLDIRTEWSDTQDPAAIWIGARTEQGTAEIEGRTLTMAPLRNRRKTETGEV
                     LISRVAEAFTEYTWEGQTGYGIMEYIERVEDGTAVGYPL"
BASE COUNT          294 a          752 c          869 g          337 t
ORIGIN      
        1 gccgacagcg agtcgttgtc gccggtcagg acgcccttga tcacgcggtt catgccgttg
       61 cgggggaggg cgtcgatgaa gtcgatctgg gtcgggacct tgtagtcggc gaggcgctcg
      121 gcgcagaagg cgatcagctc ctcgggcgtg ggggaggtgc cgggctcggg gacgacgacg
      181 ccgcgcacgt cgtggccgaa gactgggttg gggaggggga cgacggcgac ctcgaagaca
      241 gcggggtgct cgagcaggac gccctcgacc tcggccgagg agatgttgag gccgccgcgg
      301 cggatcagct gcttgttgcg gtcgacgaag aagaggaagc cgtcctcgtc gagggtcgcc
      361 gagtcgccgg tgtgcagcca gccgtcgcgc agggcgcgcg cggtttcctc ggggttgtcg
      421 acgtagcgcg acatcgtgct cggcccgcgc acgcagagct cgccgacctc gttgggcgcc
      481 gccggcgcgt cgtcctcctc gcgcagcacc gtccactcga cccactcgcg gaacccggtg
      541 cggccgatcg agagccccca ggggccgatc cgctcgagcg cctcggggtg gtcggcgtcg
      601 ctcaggtaaa tgccggtggt gccgccctcg gtcaggccgt agacgttgac cagttcgacg
      661 tcccacttgc ggccgatctc ctcccagacc cgccggtaga aggccgggct cgagggctgg
      721 gcgccgtaac agacgcggcg cagggcggag aagtcgtagg cgtcgagttc ctccggggtg
      781 cggcgttcga ggatcagcgc cagcaccgtg ttgatcagga aggtcgaggt cgtgcggtgg
      841 cgctcgatcc gctccagggt cgcgtgcacg tcgaactcgg gctcgaccac gtaggcgcag
      901 ccggcggcga cgcaggcgag caggttgctg tggcagccgg tgctggtgaa gaagggggcg
      961 aaggactggt agacggcgtt cggctccagc gcccaggcgc cgaccgactg gtacccgcag
     1021 gcgaccgagc cgcggtgggt gagcgcgacc gccttcgggt tgccggtcgt ccccgaggtg
     1081 aacagccagt cggcgtcctg gtcggggtcg agcggggtcg gctcggccag cggttcgacg
     1141 ctcgccagca gctcggccag cgggcggccg agcgcgccgg gcccggtcgt cgcctcgatc
     1201 agcgcggcgt cgccgagcgc cgggcgcagc gtcccgagcc gctcggcgaa ggcgggggcg
     1261 aagaccagcg ccgccggttc gatgaagccg agcacgtact ccagctcgcg ctgcacgtag
     1321 cgggtgttca gcggcacgtt gatcgcgccg agcaggtggg aggcgtggta ggtgacgtgg
     1381 gcctcgaccg cgccgtcgtt gtcgagcagg atcccgaccc ggtcgccggg cccgaccccg
     1441 gcggcggcca gcacgctcgc catccgcgcc gcccgctcgc cgagctggcc gtaggtcaag
     1501 gtcgtctccg cccccgcgtc gagcaggctg ggtgcgatca gggccggctt gcggccgcgt
     1561 tcggcgaccc gcttgccgag catctcgcgc acggtctgct cgggcccggg ctcgggcggc
     1621 caccagtact tgcccgtgcc cgccatgcgc ctagagcggg tagccgacgg cggtgccgtc
     1681 ctcgacccgc tcgatgtact ccatgatccc gtagccggtc tggccttccc aggtgtactc
     1741 ggtgaaggcc tcggcgaccc gcgagatcag cacctcgccg gtctcggtct tgcgccggtt
     1801 gcgcagcggc gccatggtca gcgtgcggcc ttcgatctcg gcggtgccct gctcggtgcg
     1861 ggcgccgatc cagatcgccg cggggtcctg ggtgtccgac cactcggtgc ggatgtcgag
     1921 gtcggtgacc tcgtggtact cgccgtcgat ctgcagcacc ccgagccggc gcgaggaccc
     1981 gtcgggcatg atgtttttca gcagcatgaa ggcgcggttg gggccgaagt tggcgatcag
     2041 cagccggtag gcccagatca cctgccagta gcgcgggccc caggagtggt cgcgccagcc
     2101 gtaggcgtcg ggcagcggga actcctcgtc gccgacccgg atcgagccgc gcacgccggt
     2161 gtgctggttg aagtgcccgc gtgagaactg cggcccgtag tacatgagcc gctcgtgctc
     2221 gggcgcggtc ggctcgccgc cgtgcacggg gg
//
LOCUS       DASRIG010000399         5834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1503657, whole genome shotgun sequence.
ACCESSION   DASRIG010000399 DASRIG010000000
VERSION     DASRIG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503657"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFB52_10700"
     CDS             complement(<1..856)
                     /locus_tag="VFB52_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06847.1"
                     /translation="MEGRGTNSKTLADLLQRAAEIYGDKPAVKYKDGDGWATKSFTEV
                     LEICRPLGLGLTALGVEKGDRVSILGNTRQEWTYFDFAALSIGATVVPIYQTNSPEEC
                     LYVLENSDAKVVVVEDAEQLEKVKQVRAQLPELQHVVLMVGSDPEAISMEDLAAKAAA
                     IDASVWEQRYTSVTPQDICTFIYTSGTTGPPKGCVISHGNYRAMLDMVNSTSVIEPED
                     VTYLYLPLAHSFALLIQLGSFDLGSAIAYWERDPLKILPNMAELKPTYFPSVPRIFEK
                     IYTAATSGM"
     gene            complement(873..1064)
                     /locus_tag="VFB52_10705"
     CDS             complement(873..1064)
                     /locus_tag="VFB52_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06848.1"
                     /translation="MANHLTPSELADQLHMDQQEVIGRCVQMGVPIFHGRIDRTLFEL
                     SLRSLGMMKGQQSDPEAAH"
     gene            1186..1923
                     /locus_tag="VFB52_10710"
     CDS             1186..1923
                     /locus_tag="VFB52_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZO06849.1"
                     /translation="MSEVSSEVAARPLPRRGRFWIALVLLIGVAGLGYVIVEVATTEP
                     GDELIHVEGINDAQRLFAGMPQEGDRLGSSDAPVTIQIFNDLQCSNCREDFMEAIPTL
                     AEDYARTGDVQLLYRHYSNSENVHELGFYGAEAAAEQNYGWQYIYLFFASQEEAEKFG
                     VNQNFLDTIAGGIADLDLPKWEADLEDKGGSDGEITRRLDGYEELGRNLGIRVKQAAI
                     VSGPNGTITLQDGAGLAEIEKAIAEVE"
     gene            complement(1968..2900)
                     /locus_tag="VFB52_10715"
     CDS             complement(1968..2900)
                     /locus_tag="VFB52_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO06850.1"
                     /translation="MENASTFEAHEVDLHGHRAVYRVAGSGPTVVLIHGMINSSRHWE
                     AVAAQLAQTHRVIAPDLIGHGDAATPRGDYSLGAHAAMIRDLMTTIGVERATIVGHSL
                     GGGVAMQFFYQFPQRTERLVLISSGGLGHEVSPLLRGAALPGSAALLRLATQRQIVGG
                     IAAAGAGLRAEGNKAGIYLEAVARALRPLADSGSRRAFLQTLRAVIDVEGQKVSARDR
                     LYLLGEMPTLIVWGERDRTIPLEHGLEASEAIPNCRFETLPRASHFPNLEDPDGLAAV
                     LADFLATTAPAYIDDSQWGAVITGRARAQIRRVA"
     gene            complement(2911..3531)
                     /locus_tag="VFB52_10720"
     CDS             complement(2911..3531)
                     /locus_tag="VFB52_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06851.1"
                     /translation="MADIIEQAERSGYVDLYWLPLGAGGSVVRLNGRAYEALTAMLAR
                     RRPRDLYHAALTVDTGAGRFTIEIAPVPDADGAKRGAVAEGTVGSNLAGRLRVFRYEL
                     RCWPDGTIPDLAEAVESPVRLSDDAVTAARLVEQLPLVPMPVWGRDELGAGEMWTSNS
                     TIAWLLCRAGIDAAAIALPHGGRAPGWDAGIAVAQRPGPEPVPAPR"
     gene            3533..4318
                     /locus_tag="VFB52_10725"
     CDS             3533..4318
                     /locus_tag="VFB52_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZO06852.1"
                     /translation="MSHATRTAAAAGAALLCLLAAFASLAAVAEGEVIGRSVEGRPIV
                     ATLNGDPAAPLQVLVVGSIHGNETAGMRVTRQLLARPAPRNAALRIVPTLNPDGVAAD
                     TRGNAHGVDLNRNFPYDWQPLTGGEYSGTGGLSEPESRAAHRLIRRTRPDITIWFHQP
                     FGIVDRSSGGDHRLERRFAELIGLPFVRLKGRYPGSASRWQNHAFPRDTAFVVELPTF
                     VSGALVRRSTRAVLRLAAEYASPEVGGREVARLGYCGREACTR"
     gene            complement(4288..5157)
                     /locus_tag="VFB52_10730"
     CDS             complement(4288..5157)
                     /locus_tag="VFB52_10730"
                     /inference="COORDINATES: protein motif:HMM:NF037126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06853.1"
                     /translation="MAYYSERVGGARPRRSEEIDPAFWGGVVSLVRRSVNQGSFGEDF
                     PEMCEEGQGITGCSEHDLALAVEAEFEELTWPLRESDQPQNLTVLDLLEFIYRHTSKP
                     SERNYHDFYRHQHLAFDREAGQEEMHERINRLLSRAGLIYELTYEGRIERLGSPAIET
                     QLEQSLPPTEDPTFDELLEAAVTKFHSPDQAVRREALESLWDAFERMKTMLDPNKRSG
                     AEDLLAAASQEATDEEEDLLRQEMKTLTAIGNDFRIRHHEVDAIEPTDDLAEYLFGRL
                     YAFIYRVHASLPQ"
     gene            complement(5241..>5834)
                     /locus_tag="VFB52_10735"
     CDS             complement(5241..>5834)
                     /locus_tag="VFB52_10735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO06854.1"
                     /translation="GIAIPVDCMDGQALKRAVGEAHERLGRLDALVNNVGGLVGDVER
                     LGALDHDDELFERNLFLNLTTAYYASRAALPHMAAAGYGRVLSIGSGYATHGGGTISY
                     SAAKHGVVGFSRALAYQVPPGITVNCLCPGWTNTVMLDWAGPGAREGAEAKAAALTVQ
                     GRVLSTTEPAPMATLLASPDGAGITGQVISVDGGYKV"
BASE COUNT          875 a         1939 c         2064 g          956 t
ORIGIN      
        1 ccatgccgct ggtcgcggcg gtgtagatct tctcgaagat ccgcggcacc gaggggaagt
       61 aggtcggttt cagttcggcc atgttcggca ggatcttcag cgggtcgcgc tcccagtagg
      121 cgatcgcgga gccgaggtcg aagctgccga gctggatcag cagggcgaag gagtgggcga
      181 gcggcaggta gaggtaggtg acgtcttcgg gctcgatcac gctcgtgctg ttgaccatgt
      241 cgagcatcgc ccggtagttg ccgtggctga tcacgcagcc cttcggcggc ccggtcgtcc
      301 ccgaggtgta gatgaaggtg cagatgtcct gcggggtgac cgaggtgtag cgctgctccc
      361 agaccgaggc gtcgatggca gcggctttgg cggccaggtc ctccatcgag atcgcctcgg
      421 ggtcgctgcc gaccatcagc accacgtgct ggagctcggg cagctgcgcg cgcacctgct
      481 tgaccttctc cagctgttcg gcgtcctcga cgacgaccac cttggcgtcg gagttctcga
      541 gcacgtagag gcactcctcg ggggagttgg tctggtagat cggcaccacc gtggcgccga
      601 tcgagagcgc ggcgaagtcg aagtaggtcc actcctgccg cgtgttgccg aggatcgaga
      661 cccggtcgcc tttctcgacc ccgagcgcgg tcaggccgag gccgaggggc cggcagatct
      721 ccagcacctc ggtgaagctc ttcgtcgccc agccgtcgcc gtccttgtac ttgaccgcgg
      781 gcttgtcgcc gtagatctcc gcagcacgct gcagaaggtc tgcaagcgtc ttggaattgg
      841 tacccctccc ctccatctgg ggggagtgta attcagtgag cggcttcggg gtcgctctgt
      901 tggcctttca tcatcccgag actgcgcagc gagagttcga acagcgtcct gtcgatgcgg
      961 ccgtggaaga tcgggacacc catctggacg cacctcccga tcacctcctg ctggtccatg
     1021 tgcagctggt cggcgagctc gctgggcgtc aggtgattgg ccataaagac cctcccgata
     1081 gttgaaagtt agtagcccat gtttacccct ggccccggac gtgtcaaccg ctttctgcgg
     1141 aggttgcgag gtgcccgcga gcccatctcg cgataatggc ggtggatgtc cgaagtctct
     1201 tctgaagttg ctgccaggcc gctgccccgc cgcggccgtt tctggatcgc cctggtcctg
     1261 ctgatcggcg ttgccggtct gggctacgtg atcgtcgagg tggcgaccac cgagccgggc
     1321 gacgaactga tccacgtcga aggaatcaac gatgcccagc gcctgtttgc cgggatgccg
     1381 caggaaggcg accggctcgg ctcctccgac gcaccggtga cgatccagat cttcaacgac
     1441 ctgcagtgct cgaactgccg cgaggacttc atggaagcga tcccgacgct ggccgaagac
     1501 tacgcccgga ccggcgacgt gcagctgctc taccgccatt actcgaacag cgagaacgtg
     1561 cacgagctcg gcttctacgg ggcagaggcg gcggccgaac agaattacgg ctggcagtac
     1621 atctacctct tcttcgccag ccaggaagaa gcggagaagt tcggcgtcaa ccagaacttc
     1681 ctcgacacga tcgcgggcgg gatcgccgac ctcgacctgc cgaaatggga ggccgacctg
     1741 gaagacaagg gcggctccga cggcgagatc acgcgccggc tagacggcta cgaggaactc
     1801 ggccgcaacc tcggcatcag ggtgaagcag gcggcaatcg tgagcggtcc gaacggcacg
     1861 atcacgctcc aggacggcgc cgggctggcc gaaatcgaga aagcgatcgc cgaagtcgaa
     1921 tagtcgctgt cgagttggtc aacccctggt tgacaaactc gacagggtca ggcgacgcgg
     1981 cggatctggg cgcgggcgcg gccggtgatc acggcgcccc actgcgagtc gtcgatgtag
     2041 gcgggggcgg tggtggcgag gaagtcggcg agcaccgcgg cgaggccgtc ggggtcttcg
     2101 aggttgggga agtgcgaggc gcgggggagg gtctcgaagc ggcagttggg aatcgcttcg
     2161 gaggcttcga ggccgtgctc gagcgggatc gtgcggtcgc gctcacccca gacgatcagg
     2221 gtcggcatct cgccgagcag atagaggcgg tcgcgggcgg agactttctg gccctcgacg
     2281 tcgatcaccg cgcgcagcgt ctgcagaaaa gcgcgccggg agcccgagtc ggccagcggg
     2341 cgcagcgccc gtgccaccgc ctccaggtag atgcccgcct tgttgccctc ggcgcgcagg
     2401 ccggcgccgg cggcggcgat cccgccgacg atctggcgtt gggtcgccag ccgcagcagc
     2461 gccgccgagc cgggcagggc ggcgccgcgc agcagcgggc tgacctcgtg gccgaggccg
     2521 ccgctggaga tcagcacgag ccgctcggtg cgctggggga actggtagaa gaactgcatc
     2581 gcgacgccgc cgccgagcga gtggccgacg atcgtcgccc gttcgacccc gatcgtggtc
     2641 atcaggtcgc ggatcatcgc cgcgtgggcg ccgagcgagt agtcaccgcg cggggttgcc
     2701 gcgtcaccgt ggccgatcag gtccggggcg attacccggt gcgtttgcgc gagctgcgcg
     2761 gcgaccgctt cccagtgacg ggaggagttg atcatcccgt ggatcaggac cacggttggg
     2821 ccgctgccag cgacccggta cacggcccgg tggccgtgca ggtcgacctc gtgggcctcg
     2881 aaggtgctcg cgttctccac ggagaagacc ctaacgcggg gcggggaccg gctccggacc
     2941 gggccgctgg gcgacggcga tgccggcgtc ccagccgggg gcgcgaccgc cgtgggggag
     3001 cgcgatcgcg gctgcgtcga tcccggcccg gcagagcagc caggcaatcg tcgagttcga
     3061 ggtccacatc tcgccggcgc cgagctcgtc gcgtccccag accggcatcg gcacgagggg
     3121 cagctgctcg accaggcgcg ccgccgtgac cgcgtcgtcg ctgagccgga ccgggctctc
     3181 gaccgcctcg gcgaggtcgg ggatcgtgcc gtcgggccag cagcgcagct cgtagcggaa
     3241 gactcggaga cggccggcca agttgctgcc gacagtgcct tcggcgaccg cgccccgttt
     3301 ggccccgtcc gcgtcgggga ccggggcgat ctcgatcgtg aagcggcccg cgccggtgtc
     3361 cactgtgagc gccgcgtgat agaggtcgcg cggccggcgg cgcgccagca tcgcggtcag
     3421 cgcctcgtag gcgcgtccgt tcaggcgcac gaccgacccg ccggcgccga gcggcagcca
     3481 gtagaggtcg acgtagccgc tgcgctccgc ttgctcgatg atgtcggcca ttgtgagcca
     3541 cgcgacccgc accgcggcgg cggccggcgc cgcgcttctc tgcctgctgg cggcttttgc
     3601 gtcgttggcc gcggtcgccg agggcgaggt gatcgggcgc tcggtcgagg gacggccgat
     3661 cgtcgccacc ctgaacggcg acccggcggc gccgctgcag gtgctggtcg tcggctcgat
     3721 ccacggcaac gagaccgccg ggatgcgggt cacccgccag ctgctcgcgc gcccggcgcc
     3781 gcgcaatgcg gcgctacgga tcgtccccac cctgaacccc gacggggtcg cggccgacac
     3841 ccgcggcaac gcccacggcg tcgacctcaa tcgcaacttc ccctacgact ggcaaccgct
     3901 caccggcggc gaatacagcg gcaccggcgg cctctccgag cccgagtcgc gtgctgccca
     3961 ccggctgatc cgccgcaccc ggcccgacat cacgatctgg ttccaccagc ccttcggcat
     4021 cgtcgaccgc tcctccggcg gcgaccaccg gctcgagcgc cgcttcgccg agctgatcgg
     4081 cctccccttc gtgcgcctca agggccgcta ccccggcagc gcctcgcgct ggcagaacca
     4141 cgccttcccg cgcgacaccg ccttcgtggt cgagctgccg accttcgtca gcggcgccct
     4201 cgtccgccgc tcaacgcgcg cggtgctgcg gctggcggcc gagtacgcca gcccggaagt
     4261 cgggggtcgg gaggtggccc ggctcggcta ctgcggcagg gaggcatgca cgcggtagat
     4321 gaaggcgtag agccgcccga agaggtactc ggccagatcg tcggtcggct cgatcgcgtc
     4381 gacctcgtgg tggcggatcc gaaagtcatt gccgatcgcc gtcagggtct tcatctcctg
     4441 ccggaggaga tcttcctcct catcggttgc ctcttgggag gcagcggcga gtagatcctc
     4501 ggcgccactc cgcttgttgg ggtccagcat cgtcttcatt cgctcgaatg catcccagag
     4561 cgattccagc gcctctcgcc gtacagcttg gtccgggctg tggaacttcg tgaccgcggc
     4621 ctcgagaagt tcgtcgaagg ttgggtcctc ggttggcgga agggattgct ccagctgcgt
     4681 ctcgatggct ggtgatccca gccgctcgat ccttccttcg taggtgagct catagatgag
     4741 ccccgcccgg gacagcagcc gattgatgcg ctcgtgcatc tcttcctgcc cagcctcgcg
     4801 gtcgaacgca agatgttggt gtcgatagaa gtcgtggtag ttgcgctcgg acggcttgct
     4861 cgtgtggcgg tagatgaatt ccaagagatc taggacggtg aggttctggg gttgatcgga
     4921 ctcccgcaag ggccacgtca gctcctcgaa ttcagcctcc actgcaaggg cgaggtcgtg
     4981 ttcagagcag ccggtgattc cttgcccctc ttcgcacatc tctggaaagt cctcgccgaa
     5041 cgatccctgg ttcacgctcc gtcgaaccag ggaaactacg cctccccaaa aggctgggtc
     5101 gatctcctcc gaccgtctcg gccgggctcc acccactcgt tcgctgtagt aggccacggg
     5161 gccgtcaggc tagaggtgat cggccaccgg gtgcgccaca gcgttcgcag ttcgagatat
     5221 tggggatcgc cccggccggc tcagactttg taaccgccgt cgacgctgat cacctggccg
     5281 gtgatcccgg cgccgtcggg gctggctagc agggtcgcca tcggggccgg ctcggtggtg
     5341 gagaggacgc ggccctggac ggtgagcgcc gcggctttgg cctccgcgcc ctcgcgggcg
     5401 ccggggccgg cccagtcgag catcacggtg ttggtccagc ccggacagag gcagttgacg
     5461 gtgatcccgg gcggcacctg gtaggcgagc gcgcggctga agccgacgac gccgtgcttg
     5521 gcggcgctgt aggagatcgt gccgccgccg tgggtggcgt agccggagcc gatgctgagc
     5581 acgcggccgt aaccggccgc ggccatgtgc ggcagggcgg cgcgcgaggc gtagtaggcg
     5641 gtggtcaggt tgaggaagag gttgcgctcg aacagctcgt cgtcgtggtc gagcgcgccg
     5701 agcctctcca cgtcgccgac caggccgccg acgttgttga ccagcgcgtc gagccggccg
     5761 aggcgctcgt gcgcctcgcc gaccgcccgc ttcagcgcct ggccgtccat gcagtcgacg
     5821 gggatcgcga tgcc
//
LOCUS       DASRIG010000400         5775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_430672, whole genome shotgun sequence.
ACCESSION   DASRIG010000400 DASRIG010000000
VERSION     DASRIG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430672"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFB52_10740"
     CDS             complement(<1..843)
                     /locus_tag="VFB52_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO06855.1"
                     /translation="MEASALHHASRRGLLARRSPLLKLQGDEKLVAMVRAGDPSAFEM
                     IVDRYQGRLLGFCRQMLHSTEDAEDVLQEVFVNAHRAMLRDEREINLRPWLYRIARNR
                     CLNHLRKPTADAQESMDMVPAVEASSTAERVHNREEFRQILGDVNKLPETQRSALLLR
                     EMDALSYEEIAAAMDTTVPSVKSLLVRARISLAEASQARLLTCNEVHVELSEAAEGLR
                     KVSAPVRRHIRECEECADFRSKVRSNEKMLAALFPVGPLIAFKGFIAAKLGLGGAGAS
                     TAGGG"
     gene            969..1056
                     /locus_tag="VFB52_10745"
     tRNA            969..1056
                     /locus_tag="VFB52_10745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Leu,seq:taa)
     gene            1115..2731
                     /locus_tag="VFB52_10750"
     CDS             1115..2731
                     /locus_tag="VFB52_10750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HZO06856.1"
                     /translation="MEAHGGRIVAKVLKSRGVECLFTLSGGHLFSVYDGCKEEGIRIL
                     DVRHEQTAAFAAEGIAKATRNVGVAALTAGPGVTNGLSAIAGAQANNSPVCVLGGRAP
                     ELRWGSGSLQEIDHLPFVAPLVKSAETVKEPGLIAARTAAALDLAASAPSGPTFVDYP
                     LDIVFTEAELEVPAVEAPAAAQVPAGVEEAAALLAGAERPVVMAGTGLYWGRGEEELQ
                     ALAEALGIPVFLNGLGRGCIAADHELAFSRARSTALGDADVALVVGVPMDFRLGFGQS
                     FGGETKLIRVDAAPNELTANRAPEVDLVGDVRATLAALREAAGQGSGRTRPWLEQLRN
                     VENEKRAGEQAELEDSRSPLHPVRVYKELGEVLDRDAIVVGDGGDFVSYAGRFIDTYQ
                     PGCWMDPGPFGCLGAGPGQAIGAKVAHPDRQVCLLLGDGAFGFAGMEFDTMARHGLGV
                     VGVMGNNGIWALEHHPMKFLYGYSVAAELQPETRYDELVETLGCDGVLVRHPDELRPA
                     LERAFESGRPTLVNVLTDPEIAYPRKSNLA"
     gene            complement(3161..4081)
                     /locus_tag="VFB52_10755"
     CDS             complement(3161..4081)
                     /locus_tag="VFB52_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZO06857.1"
                     /translation="MASKRVAVIDVGTNSARLLIADVDGGRVAPLERRSTVTRLGRGV
                     DLSGRLAAEAIEDACAAIGEYVEAIGELDVEAVDAIATSAVRDAENGDAFIAELRERF
                     ALSARVLDGSEEARLTYLGATAEEAPSRPTLVIDIGGGSTELIVGHGEQIDFHTSLQV
                     GVVRHSERHISSDPPAQTELEQLAIDVRGTLAATLPEGIGAEAGIAVAGTPTSLAAVE
                     MELDPYDPEQVHGHVLELRSIQRMLSRLASAPLAERVEITGLHPKRAPTIVAGVVVLV
                     ETMRAFGLERITVSEHDILYGAALEAAEPK"
     gene            complement(4071..4340)
                     /locus_tag="VFB52_10760"
     CDS             complement(4071..4340)
                     /locus_tag="VFB52_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HZO06858.1"
                     /translation="MNCPKCESEMRSYERNGVTVDQCTGCRGIFLDRGELERLIDAEA
                     TFYEREPEKSERQPERSDKGDKYEGKPRKSKKKSFLENLMELGGE"
     gene            complement(4337..5365)
                     /locus_tag="VFB52_10765"
     CDS             complement(4337..5365)
                     /locus_tag="VFB52_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO06859.1"
                     /translation="MADAGRHVASLSAGEVVFEGDLGSVRRVSARRLPVMERLSIKRL
                     VLAPNAIREPHWHANASELTYCLSGKVLVSVLDDGSASASFTVAAGEMFHVASGSLHH
                     IENVGGVEAELIVAFRNEEPEDFSFHAALGAMSDAVLGNTFGLPSSAFAAIPRDTEPA
                     YLLAREGEPEIGDRVGFGDPHHFAIEAESPPVDLSYGSARVARQQFWPALEDISMYSL
                     RISDEGMREPHWHPQTAEMGYVHEGRARMTILDPDGSTDTYELGPGDVYFVPRAYPHQ
                     IEVLGEEQIHFLVFFDQPTPGDIGYRASASVYSREVLSATLGIPVEKLPSLPFTPEDP
                     LIVPRTNP"
     gene            5426..5499
                     /locus_tag="VFB52_10770"
     tRNA            5426..5499
                     /locus_tag="VFB52_10770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5460..5462,aa:Met,seq:cat)
BASE COUNT          859 a         1912 c         2044 g          960 t
ORIGIN      
        1 gcctccgccg gccgtgctcg cgccggcgcc accgagcccg agcttggcgg cgatgaatcc
       61 tttgaaggcg atcagcgggc cgaccgggaa cagcgccgcc agcatcttct cgttcgagcg
      121 gaccttggag cggaagtcgg cgcactcctc gcactcgcgg atgtggcggc ggaccggggc
      181 ggagaccttg cgcaggccct cggcggcttc ggagagctcg acgtggacct cgttgcaggt
      241 cagcaggcgc gcctggcttg cctcggccag cgagatccgg gcccgcacca gtagcgactt
      301 gaccgagggc accgtggtat ccatcgcggc ggcgatctcc tcgtaggaga gcgcgtccat
      361 ctcgcgcagc agcagggccg agcgctgggt ctcgggaagt ttgttgacgt cgccgaggat
      421 ctggcggaac tcctcgcggt tgtggacgcg ctcggcggtc gaggaggcct cgacggccgg
      481 caccatgtcc atcgattcct gcgcgtcggc ggtcggcttg cgcaggtggt tgaggcagcg
      541 gttgcgggcg atccggtaga gccacgggcg caggttgatc tcgcgctcat cgcgcagcat
      601 cgcccggtgg gcgttgacga agacctcctg caggacgtct tcggcatcct cggtcgagtg
      661 cagcatctgg cggcagaagc cgagcagccg gccctggtag cgatcgacga tcatctcgaa
      721 ggcgctgggg tcgccggccc ggaccatcgc gacgagtttc tcgtcgccct gcagtttgag
      781 caggggcgag cggcgggcaa ggagtccgcg acgactggcg tgatgtagtg ctgaagcctc
      841 caagctctcc tccagggaag gcgttatttg ccttgtagac acggtaacag cgccggagtt
      901 gcgcactcca aacatttatt gcctttttac agggcccttt ggctctgcaa gcggtttacc
      961 atcgccaggc ccgggtggcg aaactggtca tacgcggcgc ccttaaaagg cgctgtcccc
     1021 tatgggggcg tgcgggttcg agtcccgccc cgggtattta gctgcttcga agcgcggatc
     1081 gctcaggcat cggcgtacct ataggctcgc gcccatggaa gctcacggag ggcgcatcgt
     1141 cgccaaggtt ttgaaatcac gcggggtcga gtgcctgttc acgctgtcgg gcgggcacct
     1201 gttctcggtc tatgacgggt gcaaagagga ggggatccgg atcctcgacg tgcgccacga
     1261 gcagaccgcc gccttcgccg ccgaggggat cgcgaaggcg acccggaacg tcggcgtcgc
     1321 ggcgctgacc gcggggcccg gtgtgaccaa cgggctcagc gcgatcgccg gcgcacaggc
     1381 caacaactcg ccggtctgcg tgctcggcgg gcgggcgccg gagctgcgct ggggctcggg
     1441 ctcgctgcag gagatcgacc acctgccgtt cgtcgcgccg ctggtcaagt cggcggagac
     1501 ggtcaaggag ccggggctga tcgcggcgcg gaccgcggcg gcgctcgacc tcgcagcgag
     1561 cgcgccgagc ggcccgacct tcgtcgacta tccgctcgac atcgtcttca ccgaggcgga
     1621 gctggaggtg cccgctgtgg aggcgcccgc cgccgcccag gtgccggccg gcgtcgagga
     1681 ggcggcggcg ctgctggccg gcgccgagcg gcccgtggtg atggccggca ccggccttta
     1741 ctggggtcgc ggtgaggagg agctgcaggc gctcgctgag gcgctcggca tcccagtctt
     1801 cctcaacggg ctcggccgcg gttgcattgc cgccgaccac gagctcgcct tcagccgcgc
     1861 tcgcagcacc gcgctcggcg acgccgacgt ggcgctggtc gtcggcgtgc cgatggactt
     1921 ccggctcggc ttcggccaga gcttcggcgg cgagaccaag ctgatccgcg tcgatgcggc
     1981 accgaacgag ctgaccgcca accgcgcccc cgaggtcgac ctggtcggcg acgtgcgggc
     2041 gacgctggcg gcgctgcgtg aggcggcggg gcagggcagc ggacgtacgc gcccctggct
     2101 cgagcagctg cgcaacgtcg agaacgagaa gcgcgccggc gagcaggcgg agctcgagga
     2161 ctcgcgctcg ccgctgcacc cggtgcgcgt ttacaaggag ctcggcgagg tgctcgaccg
     2221 cgacgcgatc gtggtcggcg acggcggcga cttcgtctcc tacgccggcc gcttcatcga
     2281 cacctaccag ccgggttgct ggatggaccc gggcccgttc ggctgcctcg gcgccggccc
     2341 cggccaggcg atcggcgcca aggtcgccca tcccgaccgc caggtctgcc tgctgctcgg
     2401 cgacggtgcc ttcggcttcg ccgggatgga gttcgacacg atggcccgcc acgggctcgg
     2461 cgtcgtcggc gtgatgggca acaacgggat ctgggcgctc gagcaccacc cgatgaagtt
     2521 cctctacgga tactcggtgg cggcggagct gcagccggaa acccgctacg acgagctggt
     2581 cgaaacgctc ggctgcgacg gggtcctcgt ccgccacccg gatgagctgc gcccggcgct
     2641 ggagcgcgcc ttcgagtcgg gacggccgac cctggtcaac gtgctgaccg accccgagat
     2701 cgcctacccg cgcaaatcga atctcgcctg aactcgattc agattggact gaccggtgca
     2761 cctttttggc cggtcgggag cgtcaagacc ccagaaacga gaatcggtcg ccattgctga
     2821 cgaccgattc aagctggtcc ctcaggacgg gcaggggtac gtcttcaggg accatttatt
     2881 tgatgggctc agacggcggg aggcgccatg atgcggcaat cgcccaagcc ctgtaaccag
     2941 gtgcagagcg aggataaacc cctctcgtcc ccggtcaggc gattatccgc tcggctatcc
     3001 gatgcgtcta gtcgccacga atgagcgtac caaattcctg gtagccagcg tctacccaat
     3061 tatcgatgtt cgggccttgg ggtagcccat ttgtcctact cggatcgaac tttcggtccg
     3121 gtaagtaccg gcaagttctt cgaccttacg atgaagttcc ctacttcggc tcagccgctt
     3181 cgagggccgc cccgtagaga atgtcgtgct cggagacggt gattcgctcg agcccgaagg
     3241 cgcgcatcgt ctcgaccagg acgacgacgc cggcgacgat cgtcggcgcc cgtttcgggt
     3301 gcaggccggt gatctcgacc cgctcggcga gcggcgctga ggcgaggcgg gagagcatgc
     3361 gctggatcga gcgcagctcg agcacgtggc cgtggacctg ctcggggtcg taggggtcga
     3421 gttccatctc gaccgcggcc agcgaggtcg gcgtgccggc gaccgcgatc cccgcctcgg
     3481 ccccgatgcc ctcgggcaac gtcgctgcga gcgtgccgcg cacgtcgatc gcgagctgtt
     3541 cgagctcggt ctgggccggg gggtcggagg agatgtggcg ctcgctgtgg cggacgacgc
     3601 cgacttgcag cgaggtgtgg aagtcgatct gctcgccgtg gccgacgatc agctcggtcg
     3661 agccgccgcc gatgtcgatc accagggtcg ggcgggacgg tgcctcctcg gcggtcgcgc
     3721 cgaggtaggt gaggcgcgcc tcttcggagc cgtcgaggac gcgggcggag agggcgaagc
     3781 gctcgcgcag ctcggcgatg aaggcgtcgc cgttctcggc gtcgcgcacg gcgctggtcg
     3841 cgatcgcgtc gaccgcctcg acgtcgagct cgccgatcgc ctcgacgtac tcgccgatcg
     3901 ccgcgcaggc gtcctcgatc gcctccgccg cgaggcggcc ggagaggtcg acgccgcggc
     3961 ccagccgggt gaccgtgctg cgccgctcca gcggcgcgac gcgacccccg tcgacgtcgg
     4021 cgatcagcag gcgtgccgag ttcgtgccga cgtcgattac cgcgacccgc ttactcgcca
     4081 cccagctcca tcaggttctc gaggaagctt ttctttttcg acttgcgcgg ctttccctcg
     4141 tacttgtcgc ccttgtcgct gcgctcgggc tgccgctccg acttctccgg ctcgcgctcg
     4201 tagaaggtgg cctcggcgtc gatcagccgc tccagctcac cgcggtcgag gaagattccc
     4261 cggcagccgg tgcactggtc gacggtgacg ccgttgcgct catagctgcg catctcgctt
     4321 tcgcacttgg ggcagttcat gggttggtcc gaggaacgat cagcggatcc tcgggcgtga
     4381 agggtagcga gggcagcttc tcgaccggga tcccgagggt cgcggagagc acctcgcgcg
     4441 agtagaccga ggcggaggcg cggtagccga tgtcgccggg cgtcggctgg tcgaagaaga
     4501 cgaggaagtg gatctgctcc tcgcccagca cctcgatctg gtgcgggtag gcgcggggga
     4561 cgaagtagac gtcgccgggg ccgagctcgt aggtgtcggt gctgccgtcg gggtcgagga
     4621 tcgtcatccg ggcgcggcct tcgtggacgt agcccatctc ggcggtctgg ggatgccagt
     4681 gcggctcccg catgccctcg tcgctgatcc gcagcgagta catcgagatg tcctcgagcg
     4741 cgggccagaa ctgctggcgc gcgacccggg ccgagccata gctcaggtcg accggcgggg
     4801 actcggcctc gatcgcgaag tggtgggggt cgccgaagcc gacccggtcg ccgatctcgg
     4861 gctcgccctc gcgggcgagg aggtaggcgg gctcggtgtc gcgcgggatc gccgcgaagg
     4921 ccgaggaggg cagtccgaag gtgttgccga gcaccgcgtc gctcatcgcc ccgagggcgg
     4981 cgtggaagga gaagtcctcg ggttcctcgt tgcgaaaggc gacgatcagc tcggcctcaa
     5041 caccgccgac gttctcgatg tggtgcagcg agccgctggc gacgtggaac atctcgccgg
     5101 cggcgaccgt gaacgacgcg gacgcgctgc cgtcgtcgag caccgagacc agcaccttgc
     5161 cgctcaggca gtaggtgagc tcactcgcgt tggcgtgcca gtgcggctcc cggatcgcgt
     5221 tcggtgccag cacgagcctc ttaatagaga gccgctccat gaccggcagc cgcctcgcgc
     5281 tgacccgccg caccgacccc aaatccccct cgaagaccac ttcccccgcg ctcagcgagg
     5341 ccacgtgtcg cccggcgtcg gccattgcag acatcctctc agccccgcgc ccaccggtcc
     5401 cgcattgcta ccatcgttag tccgccgcgg ggtggagcag ccaggtagct cgtcgggctc
     5461 ataacccgaa ggtcgcaggt tcgaatcctg cccccgctat aaggaaaagc cccgcatttg
     5521 cggggctttt tttcgacaca cctcgttggc tcacgccccg gcaggtacta agcaggtact
     5581 aagtttgacg tcaagggctc ctcgggcctc tggcctagct gcctcgggcc accccgtttg
     5641 ggcacgaata gctgccgtgc agctgctttt ttgcgccagt catgccaaac cttcgggttc
     5701 agcctcgaag gtctcgcttc tagggcatcg cgatgatgtc cccggggaag ttgccactgg
     5761 aaaggagaaa ggtgg
//
LOCUS       DASRIG010000401         5352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3860841, whole genome shotgun sequence.
ACCESSION   DASRIG010000401 DASRIG010000000
VERSION     DASRIG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3860841"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFB52_10775"
     CDS             <1..1401
                     /locus_tag="VFB52_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO06860.1"
                     /translation="IDVVRDGRVRITPEGQRRRYFDWMENIRPWCISRQLWWGHQIPV
                     WNRGDEVYCASEPPEGEGWERDPDVLDTWFSSALWPFATLGWPEQTPELRAFYPTDFL
                     LTARDIIFLWVARMVMMGLEFPGRVPFRDVYVHSVIQARDGRRMSKSLGTGIDPLDEI
                     DVHGADALRFGLLAMSSTQDVRFSDAKVEQGRDLANKLWNASRLILLNAAEGVEPAPR
                     PLHVEDRWLLSRLEHTIASVTEKLETYDFSHAVQEGYAFFWRDLCDWYLEIVKPRLYE
                     NEEEVSATLLYALERLLALLHPTMPFVTEEIWSFHPARQGHLAVHAFPEADESLFDQS
                     AEEDVETGIRLTQRLRAWRDLVDVPVGAMLAAALDGVEPPEFVGRLSRFEFDGVGSEP
                     VASIGPVKVLASDQLDAAAVESRLGKRREELESEVQRAERKLGNEGFVAKAPAEVVEE
                     ERGKLERYRAELAELG"
     gene            1404..2681
                     /locus_tag="VFB52_10780"
     CDS             1404..2681
                     /locus_tag="VFB52_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZO06861.1"
                     /translation="MPESAFDPDAYLDSLEPIGWRLGLERMHKLTTALGLPQHRFASI
                     HVVGTNGKSSVARMAAALLEAHGVSAGACLSPHTDRWSERTLVHDEEIDQAAWADAVE
                     RVARAAEGVNRTLEGDDAVTQFEAATAATFAALASARVKAAAIEAGLGGRLDATNVIP
                     SRVTVLTSIGLDHTEWLGETELEIAAEKLAVLRDQTTLVLGRVSAAVAELAAATAAER
                     GARLIHAPEDPGADLQLAAPGAFQRRNFALATTAVEAFLGELDQAAVARVATTLAVPG
                     RLERIAAEPPTYVDAAHNPDGAAALAEALPALAEGRRVVGCLAILADKDAAAMVQALA
                     PALDRAVCTEVPAAALAAHGRPGARSRPAAELVALCAEAGLDAEPEPDFGAALARATE
                     LAVAPPPAAVLVAGSHYGIAPARLAVSAEANVE"
     gene            2692..3132
                     /locus_tag="VFB52_10785"
     CDS             2692..3132
                     /locus_tag="VFB52_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06862.1"
                     /translation="MRWALAILVPVVLLTGGCGSASDADSTGAASDRESEVAEVIEIK
                     AEGDEIHAEMVTAIRVSKVCGAMVRTAGNRATDAWVEAVEAEESKLTDEEREAMREYT
                     SAVRCARGSLGYIRRTGKEIAALTKRLEGFDPAVIDQARSSLAP"
     gene            3142..3264
                     /locus_tag="VFB52_10790"
     CDS             3142..3264
                     /locus_tag="VFB52_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06863.1"
                     /translation="MKQRSAGSELLSMMGLVAAVVAIVILVFFGIGYLFGRLFL"
     gene            3354..4073
                     /locus_tag="VFB52_10795"
     CDS             3354..4073
                     /locus_tag="VFB52_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZO06864.1"
                     /translation="MALAIFGITNDALNLVATLFLLMLVVLWLALIVYTYLDARRRIS
                     DPFLVACATVASFFPFIGTAVYAIVRPPEFLEDAHERELEIKAAELRVRQLQDQTCPN
                     CEYPVERNYLRCPNCQRRLKDPCPTCNKPVDPRWGLCPFCETALGGGERSRRPRRRPE
                     GERPERSEGGEKRRRSAEAPPRGEPGEQSAKRPVRRKKAKPAAAKPAAERPSATETES
                     QKPTEEQRSPTREPDPDPRQA"
     gene            4042..4446
                     /gene="ndk"
                     /locus_tag="VFB52_10800"
     CDS             4042..4446
                     /gene="ndk"
                     /locus_tag="VFB52_10800"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZO06865.1"
                     /translation="MSRTLILVKPDAFERALTGEVIARFERKGLRIAAIKSLTATEEI
                     ANQHYAEHTEKPFFGELVEFITGGPLVAMVLEGTEAVPAARQMIGATNPIEAAPGSIR
                     GDFALEVTFNMVHGSDSDESAEREIGIWFPEL"
     gene            4497..4985
                     /locus_tag="VFB52_10805"
     CDS             4497..4985
                     /locus_tag="VFB52_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZO06866.1"
                     /translation="MEFEVVVPGVEELDDGDAAELVVENARRKARAIAAPADALVIAC
                     DTDVVLDGVVLGKPADQADARRYLDRMSGRPHEVLSGLVVLDGGEERSGLERTTVVFR
                     EIPEAEKQRYVAFGEWEGRSGGYAIQTLGSTLVERLEGSVSNVVGLPVGLLAELAPTL
                     FE"
BASE COUNT          804 a         1937 c         1842 g          769 t
ORIGIN      
        1 atcgacgtcg tccgcgacgg ccgtgtccgc atcacccccg agggtcagcg gcggcgttac
       61 ttcgactgga tggagaacat ccgcccctgg tgcatctcgc gccagctgtg gtggggtcat
      121 cagatcccgg tgtggaaccg cggcgacgag gtctactgcg ccagcgagcc gccggaaggg
      181 gagggctggg agcgcgaccc cgacgtgctc gacacctggt tcagctcggc actgtggccc
      241 ttcgcgacgc tcggctggcc cgagcagacg ccggagctgc gcgcgttcta cccgaccgac
      301 ttcctgctca cggcgcgcga catcatcttc ctctgggtcg cccgcatggt gatgatgggc
      361 ctcgagttcc ccggccgcgt ccccttccgc gacgtctacg tccactccgt gatccaggcc
      421 cgcgacggcc gtcgcatgtc gaagagcctc ggcaccggga tcgacccgct cgacgagatc
      481 gacgtgcacg gcgccgacgc gctgcgcttc ggcctcctgg cgatgtcctc gacccaggac
      541 gtgcgcttct ccgacgccaa ggtcgagcag ggccgcgacc tcgccaacaa gctctggaac
      601 gccagccgcc tgatcctgct gaacgccgcc gagggggtcg agccggcgcc gcggccgctg
      661 cacgtcgagg accgctggct gctctcgcgc ctcgagcaca cgatcgcctc ggtcaccgag
      721 aagctcgaga cctacgactt ctcgcacgcc gtccaggagg gctacgcgtt tttctggcgc
      781 gacctctgcg actggtacct ggagatcgtc aagccccgcc tctacgagaa cgaggaggag
      841 gtctcggcga cgctgctcta cgcgctcgaa cggctgctgg cgttgctgca cccgacgatg
      901 ccgttcgtca ccgaggagat ctggtccttc cacccggccc ggcagggcca cctcgccgtg
      961 cacgccttcc cggaggccga cgagtcgctg ttcgaccaga gcgccgaaga agacgtcgag
     1021 acggggatcc gcctgaccca gcgcctgcgc gcctggcgcg acctggtcga cgtgcccgtc
     1081 ggggcgatgc tggcggcggc actcgacggg gtcgagccgc ccgagttcgt cggccgcctc
     1141 tcgcgcttcg agttcgacgg cgtcggctcc gagccggtcg cctcgatcgg cccggtcaag
     1201 gtgctcgcct ccgaccagct cgacgccgcc gcggtcgaat cgcgcctggg caagcgccgc
     1261 gaggagctcg agtccgaggt ccagcgggcc gagcgcaaac tcggcaacga gggcttcgtc
     1321 gccaaggccc cggccgaggt ggtcgaggag gagcgcggca agctcgagcg ctaccgcgcc
     1381 gaactcgccg agctcggcta ggcgtgcccg agagcgcctt cgacccagac gcatatctcg
     1441 actcgctgga gccgatcggg tggcggctcg ggctcgagcg gatgcacaag ctgaccacgg
     1501 cgctgggcct gccccagcac cgcttcgcct cgatccacgt ggtcgggacg aacggcaagt
     1561 cctcggtcgc gcggatggcg gcggcgctgc tggaggcgca cggggtgagc gccggggcct
     1621 gcctctcccc gcacaccgac cgctggtccg agcgcaccct cgtccacgac gaggagatcg
     1681 accaggccgc ctgggccgac gcggtcgaac gggtcgcacg ggccgccgag ggggtcaacc
     1741 gcacgctcga gggcgacgac gcggtcaccc agttcgaggc cgcgacggcc gcaaccttcg
     1801 ccgccctcgc cagcgcccgg gtcaaggcgg cggcgatcga ggcggggctg ggggggaggc
     1861 tggacgcgac caacgtgatc ccctcgcggg tcacggtgct gacctcgatc gggctcgacc
     1921 acaccgagtg gctgggcgag accgagctcg agatcgccgc cgagaagctc gccgtcctgc
     1981 gcgaccagac cacgcttgtg cttggccggg tctcagccgc ggtcgccgag ctggccgccg
     2041 cgaccgcggc cgagcgcggc gcccgcctca tccacgcccc cgaggatccc ggtgccgacc
     2101 tccagctcgc cgcccccggc gccttccagc gccgcaactt cgccctcgcc acaaccgcgg
     2161 tcgaggcctt cctcggcgag ctcgaccagg ccgcggtcgc ccgggtcgcg accacgctcg
     2221 ccgtccccgg acggctcgag cggatcgccg ccgagccgcc gacctacgtc gacgccgccc
     2281 acaaccccga cggcgccgcc gcgctggccg aggcgctgcc ggcgctcgcg gaaggccgcc
     2341 gcgtggtcgg ctgcctggcg atcctcgccg acaaggacgc ggcggcgatg gtgcaggcgc
     2401 tggccccggc gctcgaccgg gcggtctgca ccgaggtgcc ggcggcggcg ctcgccgccc
     2461 acgggcgtcc cggcgctcgc tcgcgcccgg cggcggagct ggtcgcgctc tgcgcggagg
     2521 cgggcctcga cgccgagcca gagcccgact tcggcgcggc gctggcccgc gccaccgagc
     2581 tggcggtcgc gccgccaccc gccgcggtgc tggttgcggg ctctcactac gggatcgcac
     2641 cggcgcgcct cgcggtgagc gcagaggcaa acgtcgaata gtctgcccgc catgcggtgg
     2701 gcgctggcga ttctcgttcc ggttgttctc cttaccgggg gttgtggttc ggcgtcggat
     2761 gccgactcga ccggcgctgc ttccgatcgc gagtccgaag tcgccgaagt gatcgagatc
     2821 aaagccgaag gcgacgagat ccacgcggaa atggtcaccg ccatacgcgt cagcaaggtc
     2881 tgcggcgcga tggtccggac cgctggcaac cgggccaccg acgcctgggt cgaagcggtt
     2941 gaagcggaag agtcgaagct gacggacgag gagcgggagg cgatgcgcga atacaccagc
     3001 gcggtgcggt gcgccagggg cagcctcggg tacatccgcc gcaccggcaa ggaaatcgcc
     3061 gcgctgacga agcggctgga aggcttcgac ccagccgtga tcgatcaggc ccgcagcagc
     3121 cttgcgccct aagatccgcg catgaagcag cgcagcgcgg gttccgaact actctcgatg
     3181 atgggtctgg tcgcggctgt cgtcgcgatc gttatcctcg tcttcttcgg gatcggctat
     3241 ctgttcggga ggctgttcct ctaaaccccc gctgaggcgc tgaaaccgcg aacagggttc
     3301 gcgactgagg cgacgaagta gactgcccga ccctttcccg gcgggcaaat tccatggcac
     3361 tagcgatctt cggcatcacc aacgacgcgc tcaacctcgt cgcgaccctc ttcctgctga
     3421 tgctggttgt gctctggctc gcgctgatcg tctacaccta cctcgacgcc cgccgccgca
     3481 tctccgaccc cttcctggtc gcctgcgcga ccgtcgcctc cttctttccc ttcatcggca
     3541 ccgccgtcta cgcgatcgtg cgcccgccgg agttcctcga ggacgcgcac gagcgcgagc
     3601 tggagatcaa agcggcggag ctgcgcgtgc gccagttgca ggaccagacc tgccccaact
     3661 gcgagtaccc ggtcgagcgc aactacctgc gctgcccgaa ctgccagcgc cgcctcaagg
     3721 acccctgccc gacctgcaac aagccggtcg acccgcgctg gggtctctgc cccttctgcg
     3781 agaccgcgct cggaggtggc gagcgcagcc gccgtccgcg ccggcgcccg gagggcgagc
     3841 gaccagagcg ttccgagggc ggcgagaaac gccgccgttc tgccgaggcg ccgccgcgcg
     3901 gcgagcccgg cgagcaatcc gccaaacgcc cggtgaggcg caaaaaagcc aaaccggctg
     3961 ccgccaaacc cgcggcggag agaccttctg cgaccgagac cgagtcgcaa aaaccaaccg
     4021 aagaacaaag gagccccacg cgtgagccgg accctgatcc tcgtcaagcc tgacgccttc
     4081 gagcgtgccc tgaccggcga ggtaatcgcc cgtttcgagc gcaagggtct gcggatcgcc
     4141 gcgatcaagt cgctgaccgc gaccgaggag atcgccaacc agcactacgc cgagcacacc
     4201 gagaagccgt tcttcggcga gctggtcgag ttcatcaccg gcggtccgct ggtcgcgatg
     4261 gtgctggagg ggaccgaggc ggtgcctgcg gcgcgccaga tgatcggcgc caccaacccg
     4321 atcgaggccg cccccggctc gatccgcggc gacttcgcgc tcgaggtgac cttcaacatg
     4381 gtgcacggct ccgactccga cgagtcggcc gagcgcgaga tcgggatctg gttccccgag
     4441 ctgtaatcct cgcctccggc tcgccgcagc ggcgggagat cctcaccaag ctggggatcg
     4501 agttcgaggt cgtcgtcccc ggcgtcgagg agctcgacga cggggacgcg gcggagctgg
     4561 tggtcgagaa cgcgcgccgc aaggcgcgcg ccatcgccgc gcccgcggac gcgctggtga
     4621 tcgcctgcga caccgacgtg gtgctcgacg gcgtcgtgct cggcaagccc gccgaccaag
     4681 ccgacgcgcg ccgttacctc gaccgcatgt cgggccgccc gcacgaggtg ctgagtggcc
     4741 tcgtcgtcct cgatggcggc gaggagcgca gcggcctcga acgcaccacg gtggtcttca
     4801 gggagatccc ggaggccgag aagcagcgct acgtcgcttt cggcgagtgg gaggggcgct
     4861 ccggcggcta cgcgatccag accctcggct cgaccctggt cgagcgcctg gagggcagcg
     4921 tctccaatgt cgtcggcctg ccggtggggt tgctcgcgga gctggccccg accctctttg
     4981 agtagccgct aacacatgtg taggcactaa tacgcgagtt gccatcatgt ccggcaagca
     5041 gcggcgccgc gcgggcgccc aggctgcaag tccaggggca gcgtcaccgc atttgcctgc
     5101 cccgaacgag cgctccggca agatgagcgc ggcgggtttc cggtgtctcg tccggccccg
     5161 tgaaacgcaa gcaacgaacc taggtcttct tacgtgtatc gaaagcaagt acgccgccgc
     5221 agggcggtgc tggcgctgtt ggtcgtgggc tccttcgtgc tgctcacgat cacctacggg
     5281 cgcggctcga acagtctcca gagcggcgtc agcgcggtct tcagtccggt cgagtcggtc
     5341 ttcgaccggg cg
//
LOCUS       DASRIG010000402         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2266527, whole genome shotgun sequence.
ACCESSION   DASRIG010000402 DASRIG010000000
VERSION     DASRIG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266527"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3412)
                     /locus_tag="VFB52_10810"
     CDS             complement(<1..>3412)
                     /locus_tag="VFB52_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06867.1"
                     /translation="PSATSILTGVINTETPSNLAQPSLSTTTPVVGQKLTATTGTWIG
                     VPTITYEYEWQHCVGTGIHTCFTIPGADEASYVPAGGDAGLRLRVVVEATNEHGSESA
                     ASSISQKVEWPEPGSEGPPVNEVPPSLEGVAVVGGLIHADVGVWSGASFWEYAWRRCD
                     ASGESCVTIPNADQQTYSPVAADQGQTLRVVVTGHGGGQTDATSAASEPVAPPTQPVA
                     EVRPQVQGEAEIGEWLVADPGEWSGSPVISFDYRWKRCDADGRNCVRIPADPEAGSQL
                     EYSPVNADAGKTLRLEVTASNGWGSVTTSSQPTEVIPAAPPLENLALPELVYETPRFG
                     STYSTWLLGSWTGAPAIEEQWQRCDPLTADPETEAMECVDVPGADDAADMVPGVEDIG
                     FKLRLKLTATSPGEESQTVHSLPSVDPVADDVIEGDPSYTGVPAVGATITVDSGIESG
                     AGLPITTEYTFSRLTEEGPVVLQEGAADEYELTAEDAGYEIEVSILVSIWRADEVAAA
                     ETHPTSLRTPVIEVGPSNDTLPTIEGEALEGAELAADPGEWHGGGGPLTYAFQWSRCD
                     AEGESCVEIPAATDATYTATASDVGKTLIVEVTAHSGPLQASASSVPTAPFAAADAPE
                     NEVAPTISGDLVEVAIVEADQGAWSGSEPRSYSYQWQGCETADAESCLDIEDATEPQL
                     RLDPVVVDQWLRVVVTATNAAGEEQAGSELVGPVEPAPAPELSEEPSIAVLGPAEVGS
                     TLLVDGGEWENIDPAQLEYHWLRCDAAGEDCEDAGAPDDDGYRLGASDLGGTVRVEVI
                     ASNSSGEVSATSEASPEIGESTGSLAEKLVLVDEGRQSVFATTIEVAEGEEGPAEEEE
                     IADCESLTGEEDCTLLGPQISPDGKTVVIEERGGELEPGEGTLYLMNFDGSDPRVFAT
                     GSEPQWSEDGGEIYFTVAAGSGATEIVVGRADGSDADSPQPLPSPAESVESPSISAEG
                     DLLAYAGKEDLEEGNGLYLAETDGPSVRRLDLGSHLSYVSDPVFSDDGEEIIFTAASS
                     PEDWTPEDEGWWDRRQIWSIAVDGSNLHRLVPDREVAYGSPSVGPGGLVVARRSAVWV
                     SVGGGGFTDYGPVTLVQMKLGGAKPVALAGSGLDPDFLRLRYPEESGPF"
BASE COUNT          447 a         1216 c         1148 g          601 t
ORIGIN      
        1 ggaagggccc tgactcttcc gggtaccgga gccggaggaa atcggggtcc agcccggagc
       61 cggcgagcgc caccggctta gctccgccca gcttcatttg gacgagggtt accggtccgt
      121 aatccgtgaa accgccgccg ccgacgctca cccagaccgc gctccgccgc gcgaccacga
      181 gcccgccggg cccgacgctc ggcgatccat aggcgacttc ccgatcggga accaggcggt
      241 gcaggttgga gccgtcgacg gcgatcgacc agatctgccg ccggtcccac catccttcgt
      301 cttccggggt ccagtcctcg ggtgaggagg cggcggtgaa gatgatctct tcgccgtcat
      361 cggagaagac cgggtcagac acataggaca ggtgcgatcc gaggtcgagt cggcgcaccg
      421 agggcccgtc ggtctcggcc aggtagagac cgtttccttc ttccagatcc tctttgccgg
      481 cgtaggcgag caggtcgccc tcggccgaga tcgaggggct ttccacggat tccgccgggc
      541 tgggcaacgg ctgaggcgag tcggcatccg aaccgtcggc ccggccgacc acgatttcgg
      601 ttgcaccgga tccagccgcg accgtgaagt agatctcgcc gccgtcttcg gaccactgcg
      661 gttcgctgcc ggtggcgaac acgcgcgggt cggaaccgtc gaaattcatc aggtagagcg
      721 tgccttcacc gggttccagt tcgcccccgc gctcctcgat caccaccgtc ttgccgtcgg
      781 gggagatctg cggcccgagc agcgtgcagt cctcctcccc ggtcaacgat tcgcagtcgg
      841 cgatctcctc ttcttccgcc gggccttctt cgccctcggc gacttcgatc gtcgtcgcga
      901 agacgctttg gcgaccctca tcgaccagaa ccagcttttc ggccagggag ccggtcgact
      961 cgccgatctc aggactggcc tccgaggtcg cgctcacctc gccggacgag ttggaggcga
     1021 tcacttcgac tctcaccgtg ccccccaggt cggacgcgcc cagccggtac ccgtcatcgt
     1081 ccggggcccc cgcgtcctcg cagtcctctc cggcggcatc gcagcgcagc cagtggtatt
     1141 cgagttgcgc cgggtcgatg ttctcccact cgcctccgtc gacgagaagc gtcgaaccca
     1201 cctccgccgg tccgagcacg gcgatgctcg gctcctcgct cagctcgggc gccggtgcgg
     1261 gttccacggg cccgaccagt tccgaccccg cctgctcttc gcccgccgcg ttggtcgccg
     1321 tcacgacgac ccgcagccac tggtccacca cgaccgggtc gaggcggagc tgcggctcgg
     1381 tcgcgtcttc gatgtcgagg cagctttcgg cgtcggcggt ctcgcatccc tgccactggt
     1441 acgagtagct gcgcggctcc gagccgctcc aggccccctg gtcggcttcc acgatcgcga
     1501 cctcgaccag atcccccgag atcgtcggag cgacttcgtt ttccggcgcg tcggcggccg
     1561 cgaacggcgc cgtcgggacc gagctcgcgg aggcctggag cgggccgctg tgggcggtca
     1621 cctcgacgat cagcgttttg ccgacgtcgg aggcggtcgc cgtgtaggtg gcgtcggtgg
     1681 cagcggggat ctcgacgcag gattcgcctt ccgcatcgca gcggctccac tggaaggcgt
     1741 aggtgagcgg cccgccgccg ccgtgccatt cgcccgggtc cgccgccagc tcggcgccct
     1801 cgagcgcttc gccttcgatc gtcggcaggg tgtcgttgct gggcccgacc tcgatcaccg
     1861 gcgttctcag gctcgtcgga tgcgtctccg cagccgcgac ttcgtcggcg cgccagatcg
     1921 agaccaggat cgagacttcg atttcgtacc ccgcgtcttc cgcggtcagc tcgtactcgt
     1981 cggcggcgcc ttcctgcagc accaccggcc cttcttccgt cagcctggag aaggtgtact
     2041 cggtcgtgat cggaagcccc gcgcccgact cgatgccact gtcgacggtg atcgtcgcgc
     2101 ccaccgcggg cacgccggtg taagacggat cgccttcgat cacgtcgtcc gcgaccgggt
     2161 cgacgctcgg cagcgagtgg acggtctggc tttcttcgcc cggcgacgtc gcggtcagct
     2221 tcagcctcag cttgaagccg atgtcctcga cccccggcac catgtctgcg gcgtcgtccg
     2281 cacccgggac gtccacgcat tccatcgctt cggtttccgg gtcggcggtc agcgggtcgc
     2341 agcgttgcca ctgctcctcg atcgccggcg cccccgtcca ggagccgagc agccaggtcg
     2401 agtaggtgct gccgaagcgg ggcgtttcgt agacgagttc cgggagcgcg aggttttcga
     2461 gcggcggcgc cgccgggatc acctcggtcg gctgactcga cgtcgtcacc gacccccagc
     2521 cgttgctcgc cgtcacctcg aggcggagcg tcttgccggc gtcggcgttg accggcgagt
     2581 attcgagctg gcttccggct tccgggtcgg cggggatccg cacgcagttc ctgccgtcgg
     2641 cgtcgcagcg cttccagcgg tagtcgaacg agatcacggg gctgccgctc cattcaccgg
     2701 ggtcggcgac cagccactcg ccgatttcgg cttcgccctg aacctgcggc cggacctctg
     2761 ccaccggctg cgtcggcggc gcaaccggtt cggaggccgc ggaggtcgcg tcggtctggc
     2821 cgccgccgtg gccggtaacg accacgcgca gggtctgtcc ttggtcggcg gcgaccgggc
     2881 tgtaggtctg ctggtcggcg ttcgggatcg ttacgcagct ttcgccggag gcgtcgcagc
     2941 ggcgccaggc gtattcccag aaggaagcgc cggaccacac gccgacgtcg gcgtggatca
     3001 gcccgccgac gacggccacc ccttcgaggc tgggcggcac ttcgtttacg ggcggccctt
     3061 ccgaccccgg ttccggccat tcgactttct gggagatcga ggacgccgcc gattccgagc
     3121 cgtgttcgtt ggtcgcctcc accaccaccc gcagccgcaa gccggcgtcc cctccggcgg
     3181 gcacgtagct cgcttcgtcg gcgcccggga tcgtgaagca ggtgtggatg ccggtgccga
     3241 cgcagtgctg ccactcgtac tcgtaggtga tcgtgggaac gccgatccag gtgcccgtgg
     3301 tcgccgtcag tttctggccg acgaccggcg tggtggtgct gaggctgggc tgggccaggt
     3361 tcgagggagt ttcggtgttg atcacgccgg tgaggatcga ggtcgccgac gg
//
LOCUS       DASRIG010000403         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_202524, whole genome shotgun sequence.
ACCESSION   DASRIG010000403 DASRIG010000000
VERSION     DASRIG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202524"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VFB52_10815"
     CDS             <1..1364
                     /locus_tag="VFB52_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06868.1"
                     /translation="IAIAPPFNRTEFAFEWARRLQLNGKAKEAIEFYRMVPDTDKRKL
                     SARFYEMVAIQQQLDTPSTAGVDRPKLVGQIQALAAEVKKGATAALAAAQSDQEKLAN
                     RSLLVRTTLLASDIARREQKDPKKTLELLADFEKQAEGLPNQAELLGSVLFARVQSHM
                     ALGNTNEATATLVTLLKARPGGEGATIVFNLLEKLNADLDRARAAGDVEQMKVLANNR
                     AQLSGFLVDWAKNNADENIRKFTYRYSVFDAATKHLAADLEADAAARKAGLEKALALY
                     ESLQSEENTKLYHSTLPPTASAADRSAPDPAVSLGIGLIAYDLGNYPESQKRLGQLLT
                     DRKLGAPMTETIENGEPKVVENDKYWEATLKLLRSNVAIGGDAKTQSENYLKQLYIRW
                     GEQVGGTKWKPQFVKLKQELIPTFDPNTEPVDSTPATTEPTATEPTPTEPAPVETTPA
                     PAP"
     gene            1539..>2250
                     /locus_tag="VFB52_10820"
     CDS             1539..>2250
                     /locus_tag="VFB52_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06869.1"
                     /translation="MDRANLNLGVVAAALSPDARQAPRLARQLGFDGLLFDAYSPALS
                     IPDLSQSGRREFRHVLSADGRQLVGLRADLGPKGLSPGVDVDRTLSRLEKAMEAAAGL
                     QAPFLCADLGALPTPPAEAKPAKKVTQDMAGLILLPTSVEPVPEPAPAPTTISAADMA
                     LAAQVDAVLIELGARADRFGVTVAFRSDLAPFAALERAIRASGCPWFGIDLDPVAILR
                     DEWSADEVFSRLGPLIRHV"
BASE COUNT          409 a          816 c          728 g          297 t
ORIGIN      
        1 cgatcgcgat cgccccgccg tttaaccgca ccgagttcgc cttcgagtgg gcgcgtcggc
       61 tgcagctcaa cggcaaggcg aaggaggcca tcgagttcta tcgcatggtg ccggataccg
      121 ataagcgcaa gctctcggcc cggttttacg agatggtcgc catccagcag cagctcgaca
      181 cgccgagcac cgccggcgtc gatcggccga agctggtcgg gcagatccag gccctcgccg
      241 ccgaggtgaa aaagggcgcg accgccgcgc tggcggcggc gcagagcgac caggagaagc
      301 tcgccaaccg ctcgctgctc gtgcggacga cgctgctcgc gtccgacatt gcgcgccgcg
      361 agcagaagga tccgaagaag accctggagc tgctggccga cttcgaaaag caggccgagg
      421 gattgcccaa ccaggccgag ctgctcggct ccgtcctgtt cgcgcgcgtg caatcgcaca
      481 tggcgctggg gaacacgaac gaggcgacgg cgacgctggt cacgctgctc aaggcccggc
      541 cgggcggcga aggcgcgacg atcgtcttca acctgctcga gaagctcaac gccgacctcg
      601 accgcgcccg cgccgccggc gacgtggagc agatgaaggt gctggccaac aaccgcgccc
      661 agctcagcgg gttcctcgtc gattgggcga agaacaacgc cgacgagaac atccgcaagt
      721 tcacgtaccg ctacagcgtc ttcgacgccg cgacgaaaca cctcgccgcc gacctggagg
      781 ccgacgccgc cgcccgcaag gcgggcctgg agaaggcgct ggcgctgtac gagagcctgc
      841 aatcggaaga gaacacgaag ctctaccatt cgacgctccc ccccaccgcc agcgccgccg
      901 accgcagcgc gcccgacccg gcggtgtcgc tgggcatcgg cctgatcgcc tacgacctcg
      961 ggaactaccc cgagtcgcag aagcggctcg gccagctcct caccgaccgc aaactcggcg
     1021 cgccgatgac cgagacgatc gagaacggcg agccgaaagt ggtcgagaac gacaagtact
     1081 gggaagccac gctcaagttg ctccgcagca acgtcgccat cggcggcgac gcgaagacgc
     1141 agagcgaaaa ctacctcaag cagctctaca tccgctgggg cgaacaggtc ggcgggacga
     1201 agtggaagcc acagttcgtg aagctcaagc aggaactgat cccgacgttc gaccccaaca
     1261 ccgagccggt tgactcgacg ccggcgacca cagagccgac cgcgacggag ccaacaccaa
     1321 ccgaaccggc accggtcgaa accacaccgg cgcccgcgcc gtaacttcca ccctctccct
     1381 ttgggagagg gctgggggga gggcctgacc cgaacccgcg cgttgccacc gatgagagtc
     1441 aggccctcac cctgaccctc tcccaaaggg agaggggaca agaccggcgc ccttcgctcg
     1501 gttgatgttc tcccgcatcc cgcgaacaat gcgcgtcgat ggaccgcgca aacttgaacc
     1561 tcggcgtcgt ggcggcggcg ctgtcgcccg atgcccgcca agcgccgcgc ctcgcacggc
     1621 agctcggctt cgacggcctg ctgttcgacg cctactcgcc ggcgctgagc atccccgacc
     1681 tctcccagtc cggccgccgc gagttccgcc acgtgctctc tgcggacggc aggcaactcg
     1741 tcgggcttcg cgctgatctc gggccgaagg gactctcgcc gggcgtggac gttgaccgca
     1801 ccctctcgcg tctcgaaaag gcgatggaag ccgccgcggg gttgcaggcg ccgttcctct
     1861 gcgccgacct cggcgcgctg ccaacgccgc ccgcggaggc aaagcccgcc aagaaggtca
     1921 cgcaggacat ggcgggcctg atcctgctgc ccacgagtgt cgagccggtg cccgaaccgg
     1981 cgcccgcacc tacgacgata tcggcggcgg acatggcgct ggccgcgcag gtggatgcgg
     2041 tgttgatcga actcggcgcc cgcgccgacc ggttcggcgt caccgtcgcc ttccgcagcg
     2101 acctcgcccc gttcgctgcc ctcgaacgcg cgatcagggc gagcggctgc ccgtggttcg
     2161 gcatcgacct cgaccccgtc gccatcctcc gcgacgagtg gtcggcggac gaggtcttct
     2221 cgcgcctggg gccgttgatc cgccacgtcc
//
LOCUS       DASRIG010000404         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1750579, whole genome shotgun sequence.
ACCESSION   DASRIG010000404 DASRIG010000000
VERSION     DASRIG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750579"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFB52_10825"
     CDS             <1..345
                     /locus_tag="VFB52_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06870.1"
                     /translation="DRAMGPVNRDLVVANHRANVHVQDLLDVFERLGAPRSRPAAPLG
                     EMARLVRLEWRARSEIHGLLAENAHLRELLDRQAEDCAAQVETYERSLSWRLTKPLRV
                     LNALRRRLRRKR"
     gene            complement(342..1856)
                     /locus_tag="VFB52_10830"
     CDS             complement(342..1856)
                     /locus_tag="VFB52_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZO06871.1"
                     /translation="MIRIATALAVLFALVGAGSAGAATLQQVGSFDQPMYVTSDPGNP
                     DRLFVVEREGKIKEVRGGAVSLFADLTEVVSCCTQERGLQSIALAPDFATSGRLYAFY
                     TGFEGEIRIGELTASGSFAPLSTLRGVLVIAHPNHNNHYGGQLQFGSDGMLYVSTGDG
                     GGQDDEEQSAQNYESMLGKILRIDPRPVGLLPYTAPPGNPFAALPSPYDAIWSLGLRN
                     PYRFSFDRATGAMLIGDVGQGKREEVDYAPAPLAGFGSNYGWNCFEGLQPGPPVPDPE
                     CATPPPGGYTAPIFEYSHEANGEDPCAIVGGYVVRDRNVGDLDGRYVYGDYCGEQIRS
                     FEPSSPFATDRGEGIQVGQLTSFGEDSCGRVYTAQETGRVAMLVGDDGSNACPGAKPR
                     GASFVGIKAQGRRVKKGKRAQITAWVSPCAGRRGEPVKLMRGRTHLGTRRLDQVCSVR
                     FRPKITRRADFRAIIAEDANYVSATSRKLRIKVLKRKKAKRPAKQSNVRSGRLG"
     gene            1889..>2103
                     /locus_tag="VFB52_10835"
     CDS             1889..>2103
                     /locus_tag="VFB52_10835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO06872.1"
                     /translation="MSDVGGRGPAWLVLPTYNEAGNVELLVEAAISNLPSAARILIVD
                     DDSPDGTGRIADRLAAEHPAVEVLHRTR"
BASE COUNT          293 a          707 c          774 g          329 t
ORIGIN      
        1 gaccgcgcga tgggcccggt caatcgcgac ctggtcgtcg ccaaccaccg cgccaacgtg
       61 cacgtgcagg acctgctcga cgtgttcgag cggctcggcg ccccgcgctc acgtccggcc
      121 gccccgctcg gcgagatggc ccgcctggtt cgcctcgagt ggcgcgcccg cagcgaaatc
      181 cacggcctgc tggccgagaa cgcccacctc cgcgagctcc tcgaccgcca agccgaagac
      241 tgcgccgcgc aggtggagac ctacgagcga tcgctgagct ggcgcttgac caagccgctc
      301 cgggtgctca acgccctgcg gcggcggttg cgccggaagc gctagccgag ccgcccggat
      361 cggacgttgc tctgcttcgc cgggcgcttg gccttcttgc gcttcaggac cttgatgcgg
      421 agcttccgtg aggtcgcgga gacgtagttg gcgtcttcgg cgatgatcgc gcggaagtcg
      481 gcgcggcggg tgatcttggg gcggaagcgg accgagcaga cctggtcgag gcggcgggtg
      541 ccgaggtggg tgcggccgcg catcagtttg accggctcgc cgcggcggcc ggcgcagggg
      601 gagacccagg cggtgatctg ggcgcgcttg cccttcttca cccggcggcc ctgggctttg
      661 atcccgacga aggaggcgcc gcgcggtttt gctccggggc aggcgttgga gccgtcgtcg
      721 ccgaccagca tcgccactcg gccggtctcc tgggcggtgt agacgcggcc gcaggagtcc
      781 tcgccgaacg aggtcagctg gccgacctgg atgccctcgc cacggtcggt cgcgaacggc
      841 gaggagggct cgaaggagcg gatctgctcg ccgcagtagt cgccgtagac gtagcggccg
      901 tcgaggtcgc cgacgttgcg gtcgcggacg acgtagccgc cgacgattgc gcaggggtct
      961 tccccgttgg cttcgtggga gtactcgaag atcggcgccg tgtagccgcc gggcggcggc
     1021 gtcgcgcact cggggtcggg caccggcggg ccgggctgca ggccctcgaa gcagttccag
     1081 ccgtagttgg agccgaagcc ggcgagcggc gcgggcgcgt agtcgacctc ctcgcgtttg
     1141 ccctgaccga cgtcgccgat cagcatcgcg ccggtcgcgc ggtcgaagga gaagcggtag
     1201 gggttgcgca gcccgaggct ccagatcgcg tcgtagggcg agggcagggc ggcgaacggg
     1261 ttgccgggcg gggccgtgta gggcagcagg ccgaccggcc gcgggtcgat ccggaggatc
     1321 ttgccgagca tgctttcgta gttctgcgcg ctctgctcct cgtcgtcctg gccgccgccg
     1381 tcgccggtcg agacgtagag catcccgtcg gatccgaact ggagctgccc cccgtaatgg
     1441 ttgttgtggt tggggtgggc gatgaccagc actccgcgca gggtggagag cggggcgaag
     1501 ctgccggagg cggtcagttc gccgatccgg atctcccctt cgaagccggt gtagaaggcg
     1561 tagaggcggc cgctggtggc gaagtcgggg gcgagcgcga tcgactgcag cccgcgctcc
     1621 tgcgtgcagc agctgaccac ttcggtgagg tcggcgaaga gcgagacggc gcccccgcgc
     1681 acttccttga tcttgccctc acgctcgacc acgaacagcc ggtccgggtt gcccgggtcc
     1741 gaggtcacgt acatcggctg gtcgaagctg ccgacctgct gcagggttgc cgccccggcg
     1801 ctgccggccc ccaccagcgc gaagaggacg gcgagtgccg tcgcgatccg aatcactgtc
     1861 ctcaaactag ctaatagcat cgccatcaat gtccgacgtc gggggccgag gtcctgcctg
     1921 gttggtccta cccacctata acgaggcagg caacgtcgag ttgctggtcg aggcggcaat
     1981 ttccaacctg ccgtccgctg cccgcatcct gatcgtcgac gacgactcgc ccgacgggac
     2041 tggccgaatt gccgatcggc tggccgccga gcaccccgcg gtcgaggtcc tccaccgcac
     2101 ccg
//
LOCUS       DASRIG010000405        10501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_432355, whole genome shotgun sequence.
ACCESSION   DASRIG010000405 DASRIG010000000
VERSION     DASRIG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432355"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFB52_10840"
     CDS             complement(<1..341)
                     /locus_tag="VFB52_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZO06873.1"
                     /translation="MADELYKTLGVSKDASEEEIKKAYRKLARKYHPDRNPGDAEAEE
                     KFKEVSAAHDVLADPEKRKEYDAGPSFAGFGGGGGGNPFAGAGGGGFSADLGDIFSMF
                     GRGGRRARPEPV"
     gene            complement(349..870)
                     /locus_tag="VFB52_10845"
     CDS             complement(349..870)
                     /locus_tag="VFB52_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZO06874.1"
                     /translation="MSDLDETKANEVAEEPAESIEVEKDLDALLADAEAKKDEYLELA
                     KRTQADFENYRKRMASEVQAAALRGKGEMARELIEAVDNLERALESAGESPDGLAGGV
                     EMVLRGMREALSRNGIEAVDPKGEKFDPTEHEALSTLPVEGAESGLVVEVMQKGYRFG
                     EHLIRPARVVVSA"
     gene            complement(867..2756)
                     /gene="dnaK"
                     /locus_tag="VFB52_10850"
     CDS             complement(867..2756)
                     /gene="dnaK"
                     /locus_tag="VFB52_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZO06875.1"
                     /translation="MAKTIGIDLGTTNSCVAVMEGGEPTVLENAEGGRTTPSVVAFTD
                     SGERLVGTVAKRQAVMNPENTIFSIKRFMGRKESEVKEEETIVPYKVEAGPNGDVRVD
                     VRGKQYSPPEISAMILQKLKADAEAKLGEPVDSAVITVPAYFNDDQRQATKDAGKIAG
                     LDVKRIINEPTAAALAYGLDKESDDQTILVFDLGGGTFDVSVLDIGEGAFEVKATAGD
                     NHLGGDNWDKAIVEWLVAEFKRDQGVDLAADKNSLQRLYEAAEKAKIELSSTQETQIN
                     LPFITAVEGQPKHLDLKLTRSKLNELTGDLLDRVVGPVKQALGDADASIDHVVLVGGM
                     TRNPAVREKVKELTGKDPHQGVNPDEVVAIGAGIQGGVLAGDVKDVLLLDVTPLTLGI
                     ETKGGVMTKLIERNTTIPTKKSETFSTAEDNQPSVEIHVLQGEREMASGNKSLGKFQL
                     TGIAPAPRGMPQIEVTFDIDANGIINVTAKDQGTGKEQKIEIKSGSGLSDEEIQSMVS
                     DAESHAEEDRKQRELAEARNLAENAAYQAEKAIADAGDKVDQPTKDAVTEAVKDVRSA
                     VESGSLEEVNAKTEALNTAMTKASEQIYAAASQEQPTDGGSADGGSSDDEEVVDAEVV
                     DEDGK"
     gene            2963..4123
                     /locus_tag="VFB52_10855"
     CDS             2963..4123
                     /locus_tag="VFB52_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO06876.1"
                     /translation="MNLNHTHTRRGWRRHLLPAVLAVLALALMLPSLASATIFKGRVV
                     DTMTRNLYLGADLTPAIAAPSLEALTAANGQILREVTKNNFPVRAKGLAKEIRSERPD
                     LVGLQEVALWRTAPPSVVPLVTGPSATTVRYDFLAELMAELNKGFLGPHYEVVVVQQE
                     FDLEAPADENGVPGDGVPAMGIPNAEINGRLTMRDVILARKGIAVEWSNAQSGNFKTL
                     LEVPILGKPLPIARGWTSVDARILGGQKFRFVNTHLEAFHPLIRAAQAAELVAPGGPA
                     TSTDPVILLGDLNSDDDTVEGADRLAYETLLAAGFVERSTDNPLSCCINSSLLEVGGG
                     GSVADFDHQVDHVMTNDPSDVTLLDSDVTGINPVNGYWSSDHAGISSKLWVR"
     gene            4235..4528
                     /locus_tag="VFB52_10860"
     CDS             4235..4528
                     /locus_tag="VFB52_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HZO06877.1"
                     /translation="MQLYGILRRNGWRTPADLEVAGKRSAEVGDEPGSGVRWIRSYAL
                     GETSGEVGTFCVYEGESPDAIRAHAEASGLPVDEIIPIIDTVVVRPDPVPASA"
     gene            4602..7460
                     /locus_tag="VFB52_10865"
     CDS             4602..7460
                     /locus_tag="VFB52_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO06878.1"
                     /translation="MGTEAPLVGREAELARLREQLERARLGEGSLLLLCGEAGVGKSR
                     LAAALADEAGPATVLRGAAASGFTAPYGPVADALRSCLRADPAALDGCGPLRAHLALL
                     LPELGEPAGESDRATIFEAIRCALAYLAAEAPLLVVLDDLQWSDETTIELLAALAGSL
                     RELPVMVIAAYRSDGLPREHRLRWLRNELRRSGELEELTLEPFDRVTTLALLRELLAA
                     DPSPALVQAIHDRTLGSPFFVEELVVALRLRGSLREGPRGLELTEREEVPVPDTVREA
                     VLVRLSELSPEGREAVEVAAVAGQQVDLALLAELAPAEGLAELSEVGLLEETEPGRAA
                     FRHALAREAVYADVPWMRRRKLHASFAAAIEAPSGPSLELATHWLGAGDTARARAALV
                     RAAAESERLQAHRDAARAARSALELWGDGEDEELRLQTLERCGRSAELGGELGDASKA
                     WRELADSCDRSGDVLAYAHAQRHLAAVADLLGERDAAFAARRLAAEAFAGVEELAEAS
                     VEHLAMADHHRRGGRYAEAIELSRLAGEEAEAAERPDLVARALGVGGVAAAKGGEFEA
                     GLAAVQEGLALALEHELTPTAAEIYQRLSLVLYDGADYRLAEEALDTALDLCRIDGDA
                     GTEVACVTCLVYVLRERGEWARASELGQELIDSDTAVWVAQGLVGAILAFQGRLSSAR
                     RMLTESRAVSAAVGHYNMYVDSTTALAQVAAAEGLDDEAAKYCRELLARWRESEDHHY
                     AVWGVRWAAAFFATHGDREGANACAEALGRMAADAGHADALAALAQAIGEIALLDGDV
                     AAAAEHLERAVSLHRDLAVPYERAQIELRAGIALAAAGSREEALERLSDSYRTARKLG
                     AQPLAASAAREVAALGESVAQRLGARAASAADGVQLTRRELEVLRHVAVGRTNREIAQ
                     ELFISPRTVDMHVRNLLGKLECRSRVEASHRAGELGLL"
     gene            complement(7480..8667)
                     /locus_tag="VFB52_10870"
     CDS             complement(7480..8667)
                     /locus_tag="VFB52_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO06879.1"
                     /translation="MNHRIAPGRTAALALFAAAAALLACLACAGFAEAKPKKDKGTVV
                     NVMTRNLYLGADLGPALSSTSQAAFFEANGGIVRQVEATNFPVRAKGLAAEILENEPD
                     LVGLQEVALWREAPPSVGPILSGKPSAETVKYDFLALLLAQLNKKGNKYKAVVVQDEF
                     DFEAPANANGVPGDGPGGGTGLLANSEVNGRLTMRDVILARVGAGVVTSNPSSGNFVN
                     PLVVKVAGGTEVSVTRGWTAIDAKVRGSKKFHFVNSHLEAFDNEAEYPSFRAKQAGEL
                     VAPGGPADSKLPVVLLGDFNSDVKTEVKPGDGQAYRVLKQAGFRERRKPLPNTCCLST
                     SYDLKTGGGKAEFDHTVDHVMTDTPKKIKLVTSKVTGLKKAKTGWWDSDHAGVFSSLL
                     FLK"
     gene            complement(8766..9197)
                     /locus_tag="VFB52_10875"
     CDS             complement(8766..9197)
                     /locus_tag="VFB52_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZO06880.1"
                     /translation="MSKRTFIVFGLLVVVLAVAIPWLVFDGKGDAKNGAQEVPSSLEK
                     GQELFKINCGNCHTLYAAGTDGNYGPDLDELLAPNGVPPEADTEKELKEVLEPIEGRV
                     ESAIENGVDGTTTTGRMPPEILNEEQAKEVAAFVAATAGEG"
     gene            complement(9194..9886)
                     /gene="nth"
                     /locus_tag="VFB52_10880"
     CDS             complement(9194..9886)
                     /gene="nth"
                     /locus_tag="VFB52_10880"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZO06881.1"
                     /translation="MAWKRPDKRRVRAIRDRLRAMYGRPVNEPHGQPIAELIKTVLSQ
                     HTNDRNRDRSFAALRETFPSWEEVRDAPIDELEEAIRPGGLARQKAPRIQAILEQLGD
                     PPDLEWVRTAPREESMEFLLGLPGVGRKTAACVYIFTWDIPEIPVDVHVHRVGGRLGL
                     FPPKASFEKAHDEMLAIVDPADAYELHMNLIRHGRTLCRPKPRCGECELKRMCPWYRA
                     NRGAGISIPAAK"
     gene            9911..>10501
                     /locus_tag="VFB52_10885"
     CDS             9911..>10501
                     /locus_tag="VFB52_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO06882.1"
                     /translation="MSGGIEEGRVRVDGVEVFYRRVPGEGTPTVYVHGNPTHSGDWIP
                     FLERGGPALAIDLPGWGRSEKPAGFDYTMHGLSAFHERALAELGVERRKLVVHDWGAL
                     ALIGAQRRPELLERLVIMDAVPLLPGYRWHWVAQLWRRKPIGEWLNKTTTRSSVALLM
                     RQARGDRSPMPEEFIEMVWEFWDAGQQAAVLSLYRDA"
BASE COUNT         1389 a         3668 c         3657 g         1787 t
ORIGIN      
        1 accggttccg gccgcgcccg gcggccgccg cggccgaaca tcgagaagat gtcgccgagg
       61 tcggcgctga agccaccgcc gccggcgccc gcgaacgggt tgccgccacc gcccccgccg
      121 aagccggcga aggacgggcc cgcgtcgtac tccttgcgct tctcggggtc cgcgagcacg
      181 tcatgggcgg cggagacctc cttgaacttt tcctccgcct cggcgtcgcc cgggttgcgg
      241 tccgggtggt acttgcgcgc gagtttccgg tacgcctttt tgatctcctc ttcggaggcg
      301 tccttggaaa ctcccagcgt cttgtagagc tcgtctgcca cttctctcct aggcgctgac
      361 cacgacccgg gcgggtcgga tcaggtgttc tccgaatcgg tagcccttct gcatcacctc
      421 gacgacgagg ccggactcgg ccccctcgac gggcagcgtc gagagcgcct cgtgctcggt
      481 cgggtcgaac ttctcgccct tcgggtcgac ggcctcgatg ccgttgcggc tcagcgcttc
      541 gcgcatcccg cgcagcacca tctcgacccc gccggccagc ccgtccgggc tttcgccggc
      601 gctctcgagc gcccgctcga ggttgtcgac ggcctcgatc agctcgcgcg ccatctcgcc
      661 cttcccgcgc agcgccgcgg cctgcacctc gctggccatg cgcttgcggt agttctcgaa
      721 atcggcctgg gtccgcttcg ccagttcgag gtactcgtct ttcttcgcct cggcgtcggc
      781 cagcagagca tcgaggtcct tctcgacctc gatgctctcc gccggctcct cggctacctc
      841 gttggctttg gtctcgtcga ggtcgctcat ttgccgtcct cgtcgacgac ctcggcgtcc
      901 acgacttcct cgtcgtcgga gctgccgccg tcggcggaac caccgtcagt cggctgctcc
      961 tgcgaggccg ccgcgtagat ctgctccgac gctttggtca tcgccgtgtt cagcgcctcg
     1021 gtcttcgcgt tgacctcctc gaggctgccg ctctcgaccg cggagcgcac gtctttgacc
     1081 gcctcggtga ccgcgtcctt ggtcggctgg tcgactttgt cgccagcgtc ggcgatcgct
     1141 ttctccgcct ggtaggcggc attctcggcg aggttgcggg cctcggccag ctcgcgctgt
     1201 ttgcggtcct cctcggcgtg ggactcggcg tcggagacca tgctctggat ttcctcgtcg
     1261 gagaggccgg agccggattt gatctcgatc ttctgctctt tgcccgtgcc ctggtctttc
     1321 gcggtcacgt tgatgatgcc gttggcatcg atgtcgaagg tgacctcgat ctgcggcatc
     1381 ccgcgcggcg ccggggcgat cccggtgagc tggaatttgc ccaggctctt gttgccgctg
     1441 gccatctcgc gctcgccctg caggacgtgg atctcgaccg agggctggtt gtcctcggcg
     1501 gtcgagaacg tctccgactt cttcgtcggg atcgtcgtgt tgcgctcgat cagcttcgtc
     1561 atcacgccgc ctttggtctc gatcccgagg gtcagcgggg tgacgtcgag cagcagcacg
     1621 tccttgacgt cgccggccag cacgcctccc tggatcccgg cgccgatcgc caccacctca
     1681 tcggggttga cgccctggtg cgggtccttg ccggtcagct ccttgacctt ctcgcgcacg
     1741 gccgggttgc gggtcatgcc gccgaccagg acgacgtggt cgatgctggc gtcggcgtcg
     1801 ccgagcgcct gtttgaccgg gccgaccacg cggtcgagca ggtcgccggt cagctcgttc
     1861 agcttcgagc gggtcagttt caggtcgagg tgcttcggct gcccctccac ggcggtgatg
     1921 aacggcaggt tgatctgcgt ctcctgggtg gaggagagct cgatcttcgc tttctcggca
     1981 gcctcataga ggcgctgcag cgagttcttg tcggccgcga ggtcgacgcc ctggtcgcgt
     2041 ttgaactcgg cgaccagcca ctcgacgatc gctttgtccc agttgtcgcc gcccaggtgg
     2101 ttgtcgccgg cggtcgcttt cacttcgaag gcgccttcgc cgatgtccag cacggagacg
     2161 tcgaaggtgc cgccgccgag atcgaagacg aggatcgtct ggtcgtcgct ctctttgtcg
     2221 agcccgtagg cgagcgccgc cgcggtcggc tcgttgatga tccgcttgac gtcgagcccg
     2281 gcgattttgc cggcgtcctt ggtcgcctga cgctggtcgt cgttgaagta agcggggacc
     2341 gtgatcacgg cgctgtcgac cggctcgccg agcttcgctt cggcgtcggc tttcagcttc
     2401 tgcaggatca tcgcgctgat ctccggcggg ctgtactgct tgccgcgcac gtcgacgcgc
     2461 acgtcaccgt tggggccggc ctcgaccttg tacgggacga tcgtctcctc ctctttgact
     2521 tcggactcct tgcggcccat gaagcgcttg atcgagaaga tcgtgttctc ggggttcatc
     2581 acggcttgcc gtttggcaac ggtgccgacg aggcgctcgc cggagtccgt gaacgcgacg
     2641 accgacgggg tggtgcggcc gccctcggcg ttctccagga ccgtcggctc gccgccctcc
     2701 atgacggcca cgcacgagtt cgtcgtgccc aggtcgattc caatcgtctt tgccactttt
     2761 ttcgcgtcct ccaggggttt cgttgttcaa gcctatgaaa tctaagtgag agtatggcag
     2821 atcctctacc acttgccaag tgaaattgtt ccgcaggatc gcgcggactc gaaccggctg
     2881 tcaaccgggg ctctcccggg cctgtatagg gtcagcgatc agtacttact aagtactcag
     2941 atagataatc gagggagaga gaatgaacct gaatcacacg catacccgcc gcgggtggcg
     3001 ccggcacctg ctgccggccg tgctcgcggt cctggccctg gccctgatgc tgccgtcgct
     3061 cgcgtcggcg accatcttca aaggccgggt cgtcgacacg atgacccgca acctctacct
     3121 gggcgccgac ctgacgccgg cgattgccgc gccttcgctg gaagcgctga ccgcggccaa
     3181 cgggcagatc ctgcgcgaag tcacgaaaaa caatttcccg gtccgggcca aaggcctggc
     3241 caaagagatc cgctccgagc ggcccgacct ggtcggcctg caggaggtgg cgctctggcg
     3301 caccgcgccg cccagcgtcg tcccgctggt aaccggtccc agcgcgacca cggtccgtta
     3361 cgacttcctg gccgaactga tggccgagct gaacaaaggg ttcctcggac cgcactacga
     3421 agtcgtcgtc gtccagcagg agttcgacct cgaggcgccc gccgacgaga acggcgtccc
     3481 gggtgacggg gtgcccgcga tggggatccc gaacgcggag atcaacggtc gcctgaccat
     3541 gcgcgacgtg atcctcgccc gcaaaggcat cgcggtggaa tggtccaacg cacagtcggg
     3601 caacttcaaa accctgctcg aagtcccgat cctcggcaaa ccgctgccga tcgcccgcgg
     3661 ctggaccagc gtcgacgcgc gcatcctcgg cggccagaaa ttccgcttcg tcaacaccca
     3721 cctggaggcg ttccacccgc tgatccgggc cgcgcaggcg gctgagctgg tggctccggg
     3781 cggaccggcg acgagcaccg acccggtgat cctgctcggt gacctcaact ccgacgacga
     3841 caccgtcgaa ggtgcggacc ggctggctta cgaaacgctg ctcgcggcgg gcttcgtcga
     3901 gcgcagcacc gacaacccgc tgagctgctg catcaactcg agcctgctcg aagtcggtgg
     3961 cggtggcagc gtggccgact tcgatcacca ggtcgaccac gtgatgacca acgatccgag
     4021 cgacgtgacc ctgctcgact ccgacgtgac cggcatcaac ccggtcaacg gctactggag
     4081 ctccgaccac gcggggatct cgagcaagct ctgggtccgc tagcgggaat acggcatgcc
     4141 gggagcagaa aatacggcgg tttcaccgat gtgtggaagc ggcgtcgacc gtagcttcgt
     4201 ctcgtaccgc aattcgaaag cgaaggagac gaaaatgcag ctctacggaa ttctgcgtcg
     4261 caacggctgg cggacccccg ctgacctcga agtcgccggc aagcgctcgg cggaagtcgg
     4321 cgacgaaccc ggctccgggg tccgctggat ccgcagctac gcgctcggcg agaccagcgg
     4381 cgaggtcggg acgttctgcg tctacgaagg cgagagcccg gacgcgatcc gcgcccacgc
     4441 cgaggcctcc ggcctgcccg tcgacgagat catcccgatc atcgacaccg tcgtcgtccg
     4501 gccggacccg gtcccggctt cggcctgacc gatccgatcc gacccgcgcc cccgctgatt
     4561 ggcgggggcg cgggtcgttg cggtcctaga ctcagccccc gatggggacc gaagcgccgc
     4621 tcgtcggccg tgaggccgag ctcgcccggc tgcgggagca gctcgagcgg gcgcgcctgg
     4681 gggagggctc cctgctcctc ctctgcggcg aggccggcgt cggcaagagc cggttggcgg
     4741 cggcgctcgc cgacgaggcc ggccccgcga ccgtcctgcg cggcgccgcg gccagcggct
     4801 ttaccgcgcc ctatggcccc gtcgccgacg ccctgcgttc ctgtctgcgc gccgatcccg
     4861 cggcgctcga cggctgcggc ccgctgcgcg cgcacctggc gctgctgctc ccggagctcg
     4921 gcgagcccgc cggtgagagc gaccgggcga cgatcttcga ggcgatccgc tgcgcgctcg
     4981 cctacctcgc cgccgaggcg ccgctgctcg tcgtgctcga cgatctgcag tggtcggacg
     5041 agacgacgat cgagctgctg gcggcgctcg ccgggtcgct gcgcgagctg ccggtcatgg
     5101 tgatcgccgc ctaccgttcc gacgggctac cgcgcgagca ccggttgcgc tggctgcgca
     5161 acgagctgcg ccgctccggc gagctggagg agctgacgct ggagccgttc gaccgcgtga
     5221 cgaccctggc cctgctgcgc gagctgctcg ccgccgatcc ctcgccggcg ctggtccagg
     5281 cgatccacga tcgcaccttg ggctcgccct tcttcgtcga ggagctggtc gtggcgctgc
     5341 gcctgcgcgg ctcactgcgg gagggccccc gtggcctcga gctgaccgag cgcgaagaag
     5401 tgccggttcc cgacaccgtg cgcgaagcgg tcctggtgcg gctctcggag ctctcgccgg
     5461 agggccgcga ggcggttgag gttgccgccg ttgccggcca gcaggtcgac ctcgcgctgc
     5521 tcgccgagct cgccccggcc gaggggctgg ccgagttgag cgaggtcggg ctgctggagg
     5581 agaccgagcc gggccgcgcg gccttccgcc acgcgctcgc ccgcgaggcg gtctacgccg
     5641 acgtgccctg gatgcgacgg cgcaagctgc acgcgagctt cgccgccgcg atcgaagcgc
     5701 cgagcggacc gagcctggag ctggcgaccc actggctcgg cgccggcgac accgcccgcg
     5761 cccgcgccgc gctggtccgc gccgccgccg agtccgagcg cctgcaggcc caccgcgatg
     5821 ccgcccgcgc cgcccgctcg gcgctggagc tgtggggcga cggggaagac gaggagctgc
     5881 gcctgcagac cctggagcgc tgcgggcgca gcgccgagct cggcggcgag ctgggcgacg
     5941 cctcgaaagc ctggcgcgag ttggccgaca gctgcgaccg cagcggagat gtgctcgcct
     6001 acgcgcatgc ccagcgccac ctcgccgcgg tcgccgacct gctcggcgaa cgcgacgccg
     6061 cctttgccgc ccgccggctc gcagccgagg cgtttgccgg cgtcgaggag ctggccgagg
     6121 cgtcggtcga gcacctggcg atggccgacc accaccggcg cggcggccgc tacgcggaag
     6181 cgatcgagct gtcccggctg gccggcgagg aggccgaagc cgccgagcga cccgacctgg
     6241 tcgcgcgggc gctcggcgtg ggcggggtcg ccgcggccaa gggcggcgag ttcgaggccg
     6301 ggctcgctgc tgtgcaggag ggtctggcgc tggcgctcga gcacgagctg accccgaccg
     6361 cggccgagat ctaccagcgg ctcagcctcg tgctctacga cggcgccgac taccggctcg
     6421 ccgaagaggc cctcgacacg gccctcgacc tctgccggat cgacggcgac gccggcaccg
     6481 aggtcgcctg cgtcacctgc ctcgtctacg tgctgcgcga gcgcggcgag tgggcgcggg
     6541 cgagcgagct gggccaggag ctgatcgatt cggacaccgc cgtctgggtg gcgcagggcc
     6601 tggtcggcgc gatcctcgcc ttccagggcc ggctctcctc ggcgcggcgg atgctgaccg
     6661 agtcgcgcgc ggtctcggcg gcggtcggcc actacaacat gtacgtcgac tcgaccacgg
     6721 cgctggccca ggtcgccgcc gccgaagggc tcgacgacga ggcggccaag tactgccgcg
     6781 agctgctcgc gcgctggcgc gagagcgaag accaccacta cgcggtctgg ggcgtgcgct
     6841 gggccgccgc cttcttcgcc acccacggcg accgcgaggg cgccaacgcc tgcgccgagg
     6901 cgctcgggcg gatggccgcc gacgccgggc acgccgacgc gctcgcggcg ctggcgcagg
     6961 cgatcgggga gatcgccctg ctcgacggcg acgtggccgc ggccgccgag cacctggagc
     7021 gcgccgtctc cctgcaccgc gacctcgcgg tgccctacga gcgcgcccag atcgagctgc
     7081 gcgcgggcat cgccctggcc gccgcgggct cacgcgagga ggcactggag cggctctcgg
     7141 actcctaccg gaccgcgcgc aagctcggcg cccagccgct tgcggcgagc gcggcccgcg
     7201 aggtcgcggc gctcggcgag tcggtcgccc agcggctcgg cgcccgtgcc gcttcggccg
     7261 ccgacggggt gcagctgacc cggcgcgagc tcgaggtgct gcgccacgtc gcggtcggcc
     7321 gcaccaaccg cgagatcgcc caggagctgt tcatcagccc gcggaccgtc gacatgcacg
     7381 tccgcaacct gctcggcaag ctcgagtgcc gctcgcgggt cgaggcgagc caccgcgccg
     7441 gcgagctcgg gctgctctag cccgagctcg cctcgcgcgt tacttcagga acagcagcga
     7501 gctgaagacg ccggcgtggt cggagtccca ccagccggtt ttcgccttct tcagaccggt
     7561 caccttcgag gtgacgagct tgatcttctt cggcgtgtcg gtcatcacgt gatcgaccgt
     7621 gtggtcgaac tcggctttgc ccccgcccgt tttcaggtcg taggacgtgc tcaggcagca
     7681 ggtgttcggg agcggcttgc ggcgctcgcg gaacccggcc tgcttcagca cccggtaggc
     7741 ctgaccgtcg cccggcttca cttccgtttt cacgtcggag ttgaagtcgc cgagcaggac
     7801 caccggcagt ttgctgtcgg cggggccacc cggtgcgacc agctcgcccg cctgtttggc
     7861 ccggaagctc gggtattcgg cctcgttgtc gaacgcctcc aggtggctgt tgacgaagtg
     7921 gaacttcttg ctgccgcgca ccttcgcgtc gatcgcggtc cagccgcggg tgaccgagac
     7981 ttcggtgccg ccggccactt tgaccaccag cgggttgacg aagtttccgc tgctcgggtt
     8041 cgaggtgacg acgccggcgc cgacccgggc gaggatcacg tcgcgcatcg tcaggcggcc
     8101 gttgacctcg gagttggcga gcaggccggt gccgccgccg gggccgtccc cggggacgcc
     8161 gttggcgttg gcgggcgcct cgaagtcgaa ctcgtcctgg accacgaccg ccttgtactt
     8221 gttgcccttc ttgttgagct gcgcgagcag cagtgccagg aagtcgtact tgacggtttc
     8281 agccgacggt ttgccgctca ggatcgggcc gaccgagggc ggcgcttcgc gccagagggc
     8341 gacctcctgc aggccgacaa ggtccggttc gttttcgagg atctctgccg ccaggccttt
     8401 cgcgcggacc gggaagttgg tcgcttcgac ctggcgcacg atcccgccgt tggcctcgaa
     8461 gaaggccgcc tgcgaggtcg acgagagcgc cggcccgagg tccgccccga ggtagaggtt
     8521 gcgggtcatc acgttgacga ccgtgccttt gtcttttttc ggcttcgctt cggcgaagcc
     8581 agcgcaggcg aggcaggcga gcagcgcggc ggccgcggcg aacagcgcaa gcgcggccgt
     8641 gcggcccggt gcgattcggt gattcaaagt tccctcctgg tgggttcggt tccctgccgt
     8701 ccccgctcag aacaaccgta gcgcctgaaa ggtgcggggc gaaaggggga gggggctggg
     8761 agggcctagc cctcgccggc ggtggcggcg acgaaggcgg cgacttcttt cgcctgctcc
     8821 tcgttgagga tttccggcgg catacggccg gtggtggtgg tgccgtcgac gccgttttcg
     8881 atcgcgctct cgacgcggcc ttcgatcggt tcgaggactt ccttgagttc cttctcggtg
     8941 tcggcttccg gcgggacccc gttgggggcg agcagttcgt cgaggtcggg gccgtagttg
     9001 ccgtcggtcc cggctgcgta gagcgtgtgg caattgccac agttgatctt gaacagttcc
     9061 tggccctttt cgaggctgga ggggacctcc tgggcgccgt tcttggcgtc gcccttgccg
     9121 tcgaagacga gccaaggaat cgcgaccgcg agaactacga ccagcagtcc gaagacgatg
     9181 aaggtgcgct tgctcattta gccgcgggga tactaatgcc agctccccgg ttggcgcggt
     9241 accacgggca catccgcttc agctcgcact ccccacagcg cggtttcggg cggcagagcg
     9301 tgcgtccgtg gcggatcagg ttcatatgca gctcgtaggc gtcggcgggg tcgacgatcg
     9361 ccagcatctc gtcgtgggcc ttctcgaacg aggctttggg agggaagagg ccgagccgcc
     9421 cgccgacccg gtggacgtgc acgtccaccg ggatctcggg aatgtcccag gtgaagatgt
     9481 agacgcaggc ggcggttttg cggccgaccc cgggtagccc gagcaggaac tccatcgact
     9541 cctcgcgcgg cgccgtccgc acccactcga ggtcgggcgg gtcgccgagc tgctcgagga
     9601 tcgcctggat ccgcggcgcc ttctgcctcg ccaggccgcc ggggcggatc gcctcctcga
     9661 gctcgtcgat cggcgcgtcg cgcacctcct cccagctcgg gaaggtctcg cgcagggcgg
     9721 cgaaggagcg gtcgcggttg cggtcgttgg tgtgctggga gagcaccgtc ttgatcagct
     9781 cggcgatcgg ctggccgtgc ggctcgttga ccgggcgccc gtacatcgcg cgcagccggt
     9841 cgcggatcgc ccgcacgcgg cgtttgtcgg ggcgcttcca ggccatccgt ccagacctta
     9901 tggtgagggc atgtcggggg gcatcgaaga gggccgcgta cgcgtcgacg gggtcgaggt
     9961 cttctaccgg cgggtgccgg gggaggggac gccgaccgtc tacgtgcacg gcaacccgac
    10021 ccactccggc gactggatcc cgttcctgga gcgcggcggg ccggcgctgg cgatcgacct
    10081 gcccggctgg ggccgctcag agaaaccggc cggcttcgac tacacgatgc acggcctctc
    10141 ggccttccac gaacgcgccc tcgccgagct cggggtcgag cggcgcaagc tggtcgtcca
    10201 cgactgggga gcgctggcgc tgatcggcgc gcagcggcgt cccgagctcc tcgagcggct
    10261 ggtgatcatg gacgcggtgc cgctcctgcc cggctatcgc tggcactggg tcgcgcagct
    10321 ctggcggcgc aagccgatcg gtgagtggct gaacaagacg acgacccgct cctcggtggc
    10381 gctgttgatg cgccaggcgc gcggcgaccg ctcgccgatg ccggaagagt tcatcgaaat
    10441 ggtctgggag ttctgggacg ccggccagca ggcggcggtg ctctccctct accgcgacgc
    10501 c
//
LOCUS       DASRIG010000406         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3861770, whole genome shotgun sequence.
ACCESSION   DASRIG010000406 DASRIG010000000
VERSION     DASRIG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861770"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFB52_10890"
     CDS             <1..251
                     /locus_tag="VFB52_10890"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZO06883.1"
                     /translation="LDEGAARVIACATHGVFSGPAYERLADSAIERIVVTDTLPLRPG
                     APDNITVLSTAGTFADSIRRIFTDDSVSEIFAGENQLF"
     gene            256..975
                     /locus_tag="VFB52_10895"
     CDS             256..975
                     /locus_tag="VFB52_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZO06884.1"
                     /translation="MRLVGHKGADAIAPGNTLESFRAAVEAGVDTIEFDVLWLPDSHL
                     PIEQRAPLVVAHDWEDAERRTPLQLTEVLDAFLEPPLDQVEFDLDIKLPGREEEFVNA
                     LRERGLSQRAMISTMELFTLGKVLELAPELRRGWTYPKVTKDWTRKRWAKGPMLAALV
                     GMRHRLPGLAAQKLPQMGVEAMWVYHPLVTPRLARICKLAKVELIAWTVDDAARMQKL
                     IDAGVDGICSNDPRLFASLTR"
     gene            complement(965..1690)
                     /locus_tag="VFB52_10900"
     CDS             complement(965..1690)
                     /locus_tag="VFB52_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06885.1"
                     /translation="MNLVHSEWIKLLTTRTTLVMIGVGLLCEGLFAGLLTGLASFEDI
                     GEVTELITGLGLLMTLMLVLGVLIITTEFRHGTTGSTFLVTPKRWPVMLAKLGAALLI
                     GVLAGLLFVLVNGGLALPIYSGRGGTLPPTDEIVKVYAGVIASFALLAAFGLGIGAII
                     RNQVGAIIAAIAVFFVVSGLPELLPGTIGEYFPAQTVGTLHGRVEGDGTLTQVGAGLI
                     LAAWSFALVAIGTALTVVRDLSE"
     gene            complement(1687..2592)
                     /locus_tag="VFB52_10905"
     CDS             complement(1687..2592)
                     /locus_tag="VFB52_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO06886.1"
                     /translation="MPELEVRNLTKRYGGTVAVDDLSFSVDAGRVTGFLGPNGAGKTT
                     TMRALLGLLRPTRGEALVEGQPPAAMERPLHTIGAALEATAFHPGRSGRNHLRSLAAA
                     AKIPTSRVDEVLEMVELTGAAGRRVKGYSLGMRQRLALAAALLGDPHILILDEPANGL
                     DPQGMRWLRDLLKAQAAEGRTVLVSSHVLSEVAQTADELILIRKGKLVAQTTLAEFTA
                     GGRAAMRVRAADPEKLATALAERSGTVGRDEDGALLVEGLSGEQVGELALERGIALYE
                     LAPQRSSLEARFLEVMGEDAEETAP"
     gene            complement(2646..>3430)
                     /locus_tag="VFB52_10910"
     CDS             complement(2646..>3430)
                     /locus_tag="VFB52_10910"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HZO06887.1"
                     /translation="LYGALVTIPLAIVFGGPGLYLLDQKSYAGAIPILVVGIYVLSVV
                     GFFFAVGLAAAANQIFQGKEATVSDGLAVARERFPEICGWAALSTAIGLIMALLENQG
                     GALGSIAARLVGLAWALVTFMAVPVLAIEGPGPFQTLKRSGTIFKDKWGQQITGNIAI
                     GGAIGLLAILPAGIMIGVGVALWSKSGTAAVVLILVGAVIMCVALLISKALSGVFGVA
                     LYRYAVEGEAVGGFTSEELESAVKLKKGRRGEAPPTASPGTV"
BASE COUNT          522 a         1201 c         1228 g          479 t
ORIGIN      
        1 tgctcgacga gggtgccgcg cgggtgatcg cctgcgccac ccacggcgtc ttctccggcc
       61 ctgcctacga gcggctcgcc gactcggcga tcgagcggat cgtcgtcacc gacaccctgc
      121 cgctgcggcc cggagccccg gacaacatca cggtgctctc cacggccggc accttcgccg
      181 actcgatccg acgcatcttc accgacgact cggtctccga gatcttcgcc ggcgagaacc
      241 agctcttcta ggcgcttgcg tctggtcggc cacaagggcg cggatgcgat cgcgcccggc
      301 aacacgctgg agagcttccg ggccgcggtc gaggccggcg tcgacacgat cgagttcgac
      361 gtgctctggc tgcccgactc gcacctgccg atcgagcagc gggcgccgct ggtcgtcgcc
      421 cacgactggg aggacgccga gcgccgcacg cccctgcagc tgaccgaggt gctcgacgcc
      481 ttcctcgagc cgccgctgga tcaggtcgag ttcgacctcg acatcaagct gcccggccgc
      541 gaggaggagt tcgtcaacgc cctgcgcgag cgcggcctga gccagcgggc gatgatctcg
      601 acgatggagc tgttcacgct cggcaaggtg ctcgagctgg cgccggagct gcggcgcggc
      661 tggacctatc cgaaagtgac caaggactgg acccgcaagc gctgggcgaa ggggccgatg
      721 ctggcggcgc tggtcgggat gcgccaccgc ctgccgggcc tggcggcgca gaagctgccg
      781 cagatggggg tcgaggcgat gtgggtctat cacccgctcg tcacgccgcg cctggcgcgg
      841 atctgcaagc tggccaaggt cgagctgatc gcctggaccg tcgacgacgc cgcccggatg
      901 cagaagctga tcgacgccgg agtcgacggc atctgctcca acgacccgcg ccttttcgct
      961 tcgctcactc gctgaggtcg cggaccacgg tcagcgcggt gccgatcgcg accagggcga
     1021 acgaccaggc ggcgaggatc aatccggccc cgacctgggt cagcgtgccg tcgccttcga
     1081 cccggccgtg cagggtgccg acggtctgcg ccgggaagta ttcgccgatc gtgccgggca
     1141 gcagttcggg caggccggag accacgaaga agacggcgat cgcggcgatg atcgccccga
     1201 cctggttgcg gatgatcgcg ccgatcccga gcccgaaggc ggccagcagg gcgaaggagg
     1261 cgatcacccc ggcgtagacc ttcacgatct cgtcggtcgg cggcagggtg ccgccgcggc
     1321 ccgagtagat cggcagggcg aggccgccgt tgacgagcac gaagagcagg ccggcgagca
     1381 cgccgatcag cagcgcggcg ccgagcttcg ccagcatcac cggccagcgc ttgggcgtga
     1441 ccaggaaggt cgagccggtg gtgccgtggc ggaactcggt ggtgatgatc agcacgccga
     1501 gcaccagcat cagcgtcatc agcaggccga ggccggtgat cagctcggtc acctcgccga
     1561 tgtcctcgaa ggaggcgagg ccggtgagca gcccggcaaa gagtccctcg cagagcaggc
     1621 cgacaccgat catcaccagg gtcgtgcggg tcgtcagcag cttgatccac tcgctgtgca
     1681 ccaggttcat ggggccgtct cctcggcgtc ctcgcccatc acctcgagga agcgggcctc
     1741 gaggctggaa cgctgcggcg ccagctcgta gagggcgatc ccgcgctcca gcgccagctc
     1801 gccgacctgc tcgccggaga ggccctcgac cagcagcgcc ccgtcctcgt cgcggccgac
     1861 cgtcccgctg cgctccgcga gcgcggtggc cagcttctcg gggtcggcgg cgcgcactcg
     1921 catcgccgcc cggccgccag ccgtgaactc ggccagggtc gtctgggcga ccagcttgcc
     1981 cttgcggatc aggatcagtt cgtcggcggt ctgagcgacc tcggagagca cgtggctgga
     2041 gaccagcacg gtgcgccctt cggccgcctg cgctttcagc aggtcgcgca gccagcgcat
     2101 gccctgcggg tcgagcccgt tggcgggctc gtcgaggatc aggatgtggg gatcgccgag
     2161 cagcgccgcg gccagggcga ggcgctggcg catccccagc gagtagccct tcacgcgccg
     2221 cccggcggcg ccggtgagct cgaccatctc gagcacctcg tcgactcgcg acgtcgggat
     2281 cttcgccgcc gccgcgaggc tgcgcaggtg gttgcggccg ctgcggccgg ggtggaaggc
     2341 ggtcgcctcg agggcggcgc cgatcgtgtg caggggtcgc tccatcgccg cgggcggctg
     2401 gccctcgacc agggcttcgc cccgggtcgg ccgcagcagc ccgagcaggg cccgcatcgt
     2461 cgtcgtcttg ccggctccgt tgggaccgag gaagccggtc acgcggccgg cgtcgacgct
     2521 gaacgagagg tcgtcgaccg cgacggtccc gccgtagcgc ttggtgaggt tgcggacctc
     2581 gagctcaggc atgcctggga ccgtattgag tattgagatc cggtggcgag cgctcgcggc
     2641 gaagctcaga cggtgccggg gctcgccgtc ggcggtgcct cgccgcgccg gccctttttc
     2701 agcttcaccg cggactccag ctcctcggag gtgaagccgc cgaccgcttc gccctcgacc
     2761 gcgtagcgat agagggcgac gccgaagacg ccggagagcg ccttcgagat caacagcgcc
     2821 acgcacatga tcaccgcgcc gaccaggatc aggacgaccg ccgcggtccc ggatttcgac
     2881 cagagcgcga ccccgacgcc gatcatgatc cccgcgggga ggatggcgag cagcccgatc
     2941 gcgccgccga tcgcgatgtt gccggtgatc tgctgacccc atttgtcctt gaagatcgtg
     3001 cccgagcgtt tcagcgtctg gaacgggccg gggccctcga tcgcgagcac cggcacggcc
     3061 atgaaggtga ccagcgccca ggcgagcccg accagacggg cggcgatgct gccgagcgcg
     3121 ccgccctggt tctcgagcag cgccatgatc aggccgatcg cggtcgagag cgccgcccag
     3181 ccgcagatct cggggaagcg ttcgcgggcg acggcgagcc cgtcggagac ggtcgcctct
     3241 ttgccttgga agatctggtt cgccgccgcc gcgaggccga ccgcgaagaa gaagccgacc
     3301 acgctgagca cgtagatgcc gacgaccaga atcgggatcg cgccggcgta gctcttctgg
     3361 tcgagcaggt agaggccggg accgccgaac acgatcgcca gcgggatcgt gaccagggcg
     3421 ccgtagagcg
//
LOCUS       DASRIG010000407         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3861915, whole genome shotgun sequence.
ACCESSION   DASRIG010000407 DASRIG010000000
VERSION     DASRIG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861915"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFB52_10915"
     CDS             <1..200
                     /locus_tag="VFB52_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06888.1"
                     /translation="LYEHSTFTQGAVWGIDSFDQWGVELGKALAVAIIPELQADEEPR
                     LAHDSSTNALIRRYRQARRAE"
     gene            204..1595
                     /locus_tag="VFB52_10920"
     CDS             204..1595
                     /locus_tag="VFB52_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZO06889.1"
                     /translation="MPLLDSLPLAATESNLLAARELMAFTLAFHIVLACIGVALPAII
                     LTANWLGLKRGDEDAMRLARRWSKAMAVTFAVGAVTGTVLSFEFGLLWPRFMERFGDA
                     FGIAFAIEGIFFFVEAIFLAIYIYGWKRLGGWAHFWSGVPMVITGIGGAFSVVTANAW
                     MNQPQGFTLNKAGEVIDVEPLEVLFNPAFGYEVPHMILAAYMVVGFLVASIYAVGMLK
                     GRRDRLHRLGLVIPLAIGCIATPIQLFVGDTAAREVADHQPAKFAAMECIYETGPNQT
                     EWLGGICTDEEVKFGIGIPDLDSFLVGWSADTVVTGLDKIPDDEQPPAHTLLHLSFDL
                     MVGVGTGLMFLALWFGWVWWKKRDIPQTPWFLRAVSISGVGAILALWAGWIVTEVGRQ
                     PWIVQGYMKTEEAVTEAEGIWFAFALILLLYAALGTTAILVLRGMSKRWREQGGADVE
                     QGTPYAPPVEEGS"
     gene            1592..>2006
                     /locus_tag="VFB52_10925"
     CDS             1592..>2006
                     /locus_tag="VFB52_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZO06890.1"
                     /translation="MSKADAAAAILWVGATFYALFGGADFGGGLWDLIAGDAEKGRAP
                     RMLIQRSLTPVWEANHVWLIFVLVVLWTAFPSAFKAIFTTLYVPIALAALGIVLRGAG
                     FAFRKSVVTLNEERTAGAIFALSSVITPFFMGVVVG"
BASE COUNT          275 a          698 c          685 g          348 t
ORIGIN      
        1 cgctctacga gcacagcacc ttcacccagg gagccgtgtg gggcatcgac tccttcgacc
       61 agtggggggt cgagctgggc aaggcgctgg ccgtggcgat catccccgag ctgcaggctg
      121 acgaggagcc gcggctcgcc cacgacagct cgaccaacgc gctgatccgc cgctaccgcc
      181 aggcgaggcg cgcggagtaa gccgtgcctc tgctcgactc cctccccctg gcggcgacgg
      241 agtcgaactt gctcgccgcg cgcgagctga tggccttcac cctggccttc cacatcgtgc
      301 tggcctgcat cggcgtcgcc ctgccggcga tcatcctcac cgccaactgg ctcggcctga
      361 agcgcggcga cgaggacgcg atgcgcctgg cgcggcgctg gtcgaaggcg atggcggtca
      421 ccttcgcggt cggcgccgtc accggcaccg tgctgagctt cgagttcggc ctgctctggc
      481 cgcgcttcat ggaacgcttc ggcgacgcct tcggcatcgc cttcgcgatc gagggcatct
      541 tcttcttcgt cgaggcgatc ttcctggcga tctacatcta cggctggaag cggctcggcg
      601 gctgggccca tttctggtcc ggcgtgccga tggtgatcac cggcatcggc ggcgccttct
      661 cggtggtcac cgccaacgcc tggatgaacc agccgcaggg cttcaccctg aacaaggccg
      721 gcgaggtgat cgacgtcgaa ccgctggaag tcctcttcaa cccggccttc ggctacgagg
      781 tcccgcacat gatcctcgcc gcctacatgg tcgtcggctt cctggtcgcc tcgatctacg
      841 cggtcgggat gctgaagggc cggcgcgacc ggctgcaccg gctggggctg gtgatcccgc
      901 tggcgatcgg ctgcatcgcg accccgatcc agctcttcgt cggcgacacc gcggcgcgcg
      961 aagtcgccga ccaccagccg gccaagttcg cggcgatgga gtgcatctac gagaccgggc
     1021 cgaaccagac cgagtggctc ggcgggatct gcaccgacga agaggtcaag ttcgggatcg
     1081 ggatccccga cctcgactcc ttcctggtcg ggtggagcgc cgacaccgtg gtcaccggcc
     1141 tcgacaagat ccccgatgac gagcagccgc ccgcgcacac gctgctgcac ctctccttcg
     1201 acctgatggt cggggtcggc accgggctga tgttcctggc cctgtggttc ggctgggttt
     1261 ggtggaaaaa gcgcgacatc ccgcagacgc cgtggttcct gcgcgcggtc tcgatctccg
     1321 gcgtgggggc gattctcgcc ctctgggcgg gctggatcgt gaccgaggtc gggcgacaac
     1381 cgtggatcgt ccagggctac atgaaaaccg aggaagcggt gaccgaggcg gaggggatct
     1441 ggttcgcctt cgcgctgatc ctgctgctct acgcggccct cggcacgacc gcgatcctgg
     1501 tcctgcgcgg gatgtcgaag cgctggcgcg agcagggtgg ggcggacgtc gagcagggca
     1561 ccccctacgc gccgccggtt gaggaggggt cgtgagcaag gccgacgccg ccgcggcgat
     1621 cctctgggtc ggggcgacct tctacgcgct gttcggcggc gccgacttcg gcggcggcct
     1681 ctgggacctg atcgccggcg acgccgagaa ggggcgggcg ccgcggatgc tgatccagcg
     1741 ctcgctgacc cccgtctggg aggccaacca cgtctggctg atcttcgtcc tcgtcgtcct
     1801 ctggaccgcc ttcccctcgg ccttcaaagc gatcttcacg accctctacg tgccgatcgc
     1861 gctggccgcg ctggggatcg tcctgcgcgg cgccggcttc gccttccgca aatcggtggt
     1921 gaccctgaac gaggaacgca cggcgggcgc gatcttcgcc ctctcctcgg tgatcacccc
     1981 gttcttcatg ggcgtcgtgg tcggcg
//
LOCUS       DASRIG010000408         5760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_203709, whole genome shotgun sequence.
ACCESSION   DASRIG010000408 DASRIG010000000
VERSION     DASRIG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203709"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFB52_10930"
     CDS             complement(<1..567)
                     /locus_tag="VFB52_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06891.1"
                     /translation="MRDLTAEVKAPANGSAPAATGRRRRGGQRSGSSLTVKVGAVVLT
                     FAAFLLFLQYGLVHLGVETYIVPKPDTVISALNDYWATDLRDALWFTAREVVFGLLLG
                     VMVGILLAILIDALPLLEYVILPYVIALVMTPMIALVPVVFLQFGDTLLVRICVVAFA
                     AAPMMMLNTLTGLRRTPEIRLDVMRALRA"
     gene            complement(555..1679)
                     /locus_tag="VFB52_10935"
     CDS             complement(555..1679)
                     /locus_tag="VFB52_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO06892.1"
                     /translation="MPPSIRAIRRTPILVAVLLAALVFAIAGCGSDGDDSSSSSTEPA
                     ATGSDSSTTPTEVKDVHFVMPVPPGPDLAAYYIADEQFGPENGIKFEFDTQLVATQGA
                     KLIVSGQADAFLGITTVLAVVNDAGMPLKYFRDDFVRNLTGFAVPADSDIKEIADLKG
                     KKIGIIDAGWPIVWDPIMRAAGLEPDEYEYVVVGVGNYQAMAAGRVDAVASWAMNWQP
                     PNNSIEPLRYLPGSDLPEVARTPSNGVMVKDEVIAEKPEWLIGLGRAISQAQEFMKAN
                     PKCAAEIALKYVPSEEPFEVTLSQIEAQIPYTYNKATEEHGQGYSDPQAFEYMLEDMY
                     EGGVIKTPLKAEDVIDNALIPEIDDFDEQAVVEKAEACEI"
     gene            1923..3071
                     /locus_tag="VFB52_10940"
     CDS             1923..3071
                     /locus_tag="VFB52_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06893.1"
                     /translation="MPAERLLPSEEATAVVALARDFAASELAPRAARAEAEGRFPRDV
                     FARLGELGLMGMPYEEEIGGGLSYEVYLQVLEELAGAWATVAIGVSVHVMSCFALSRH
                     GSAEARERWLGEMLGGELLGGYCLSEADAGSDAAAMRTRARRQGESFRIDGSKAWITH
                     GGEADFYTLFARTSDDRTRGISCFLVPGDAAGISARAPERKMGLTGSVTAALDWDGVE
                     VDAERLVGAEGAGLPIALEALDSGRLGIGAIAVGVAQRALDHAVAYAGERHAFGRPIA
                     EHQGLGFLLADMAAAVESARATVLEAARRRDRGLPFSRQASIAKLVATDAAMSVTTDA
                     VQVLGGYGYTQDFPVERLMREAKAMQIFEGTNQIQRMVIARQLGDPAR"
     gene            complement(3140..4363)
                     /locus_tag="VFB52_10945"
     CDS             complement(3140..4363)
                     /locus_tag="VFB52_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZO06894.1"
                     /translation="MSAPGGILAGMRVVEGSAFVAAPLGGMTLAQLGAEVIRFDQIGG
                     GLDHGRWPLAADGQSLFWAGLNKGKRSIALDLRSPEGRELATELIAAPGPEAGLFLTN
                     FPARGWLGYETLRERRSDLIMVALTGNPDGTSEVDYTVNPATGFPWATGPRNLAEPLN
                     SVLPAWDVAMGTLAAVGLLAAERHRTRTGEGQKIGLALSDVAFAMVGNLGRIAEATLG
                     ATDQPKDGNYLYGAFGHDFETRDGRRLMVVALTARQWEALVEVTGIAEACRGVEAATG
                     HDLASESGRFEGRDLIAALLRPWFASRELAAIRAAFAGTNVSWGPYQTFRQLVDEDPR
                     VSTENPMFEAVTHPGVGEYLMPGSPLDFSTLERAPVRRAPQLGEHTEQVLAEVLGLGG
                     AEIGRLFDAGVVAGA"
     gene            complement(4360..5364)
                     /locus_tag="VFB52_10950"
     CDS             complement(4360..5364)
                     /locus_tag="VFB52_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZO06895.1"
                     /translation="MGEPSAAPFFEDLRVGQVLDGAPGLTLTDGHAALHQAILGDRLR
                     IALDAELSSRVLGPGPALAHPGLVCDVAIGQSTQLTQRVIGNLFYRGLSLLRAPRLGD
                     TLRTTTEVVALRQNSAKPGRAATGLAVLRIRTRDQEGREVLDFERCAMLPLRDPEGET
                     GHADEIRTGTEEPSAEQLGAALSGWDLAAYREAVPGPHFADLSPGQRWTVEGADLVSS
                     APELARLSLNVAFAHHDAATTGHGRRLVYGGHTIGIAAAQLTRAIPGLLTIVAWHSCD
                     HLGPVFEGEMLRSEVELERVEPLATGGGLAHLRSRVRALREDGEEAAVLDWRLVGALA
                     "
     gene            complement(5357..>5760)
                     /locus_tag="VFB52_10955"
     CDS             complement(5357..>5760)
                     /locus_tag="VFB52_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06896.1"
                     /translation="ATPPVSGAVELVGTLAGMPGYSGRVVATVTSPHDMAARMLDVRP
                     AKGDLDELTEVAADLLAGLVEAYAGAGASMVVMANWTPAPDAGTLAPLERAAAHARVD
                     AVSWDVAEPIAGDLAIVRVAADTPLAELGGG"
BASE COUNT          780 a         1955 c         2157 g          868 t
ORIGIN      
        1 cgcccgcagc gcccgcatca cgtcgaggcg tatttccggc gttcgccgca gcccggtgag
       61 cgtgttcagc atcatcattg gcgccgccgc gaaggcgacc acgcagatcc gcaccagcag
      121 ggtgtcgccg aactgcaaga agaccaccgg caccagggcg atcatcggcg tcatcaccaa
      181 ggcgatcacg tagggcagga tcacgtactc gagcagcggc agagcgtcga tcaggatcgc
      241 caggaggatc ccgaccatga ccccgagcag gagcccgaag acgacctcgc gggcggtgaa
      301 ccagagcgcg tcgcgcaggt cggtggccca gtagtcgttc agcgcggaga tgaccgtgtc
      361 cggcttcggg acgatgtagg tctcgacccc gaggtggacc agcccgtact ggaggaagag
      421 caggaaggcg gcgaaggtga ggacgaccgc ccccaccttc acggtcaggg agctgccgct
      481 ccgctggccg ccccgccgcc tccgaccggt ggcggccggc gccgacccgt tggccggcgc
      541 cttcacctcc gcggtcagat ctcgcatgct tcagcctttt cgaccaccgc ctgttcgtcg
      601 aagtcgtcga tctccgggat cagcgcgttg tcgatcacgt cctcggcttt cagcggggtt
      661 ttgatcacgc cgccttcgta catgtcttcg agcatgtatt cgaaggcctg cgggtcgctg
      721 tagccctggc cgtgttcttc ggtcgccttg ttgtacgtgt aggggatctg ggcttcgatc
      781 tgggagagcg tgacctcgaa cggttcctcg gagggcacgt atttgagggc gatttcagcc
      841 gcgcatttcg ggttggcttt catgaactcc tgcgcctggg agatcgcccg gccgaggccg
      901 atcagccatt ccggtttttc ggcgattacc tcgtctttga ccatcacccc gttggagggg
      961 gtgcgggcga cttcgggcag gtcggagccg ggcaggtaac gcagcggctc gatcgagttg
     1021 ttcggcggct gccagttcat cgcccagctg gcgaccgcgt cgacccggcc ggcggccatc
     1081 gcctggtagt tgccgacgcc gacgacgacg tattcgtatt cgtcgggttc gagcccggcg
     1141 gcgcgcatga tcgggtccca gacgatcggc cagccggcgt cgatgatccc gatcttcttg
     1201 cctttgaggt cggcgatctc cttgatgtcg gagtcggccg ggacggcaaa gccggtgagg
     1261 ttgcggacga agtcgtcgcg gaagtacttg agcggcatcc cggcgtcgtt gacgacggcc
     1321 agcacggtgg tgatcccgag gaaggcgtcg gcctgtccgc tgacgatcag tttcgcgccc
     1381 tgggtcgcga ccagctgcgt gtcgaactcg aacttgatgc cgttctcggg gccgaactgt
     1441 tcgtcggcga tgtagtacgc ggcgaggtcg gggccgggcg gcaccggcat cacgaagtgg
     1501 acgtctttga cctcggttgg cgtcgtcgac gagtcgctgc cggtggcagc gggttcggtg
     1561 ctgctggagc tgctgtcgtc tccgtcggaa ccacagccgg cgatcgcgaa aaccaacgcg
     1621 gccagcagca cggcgactag aatcggggtc cttcgtattg ctcgaatgct cggtggcatc
     1681 gcatctcctc ctaagggatc gcctgccacc cgtcgagcgc gaagcccacg cgccgccctg
     1741 tgcggcgcgc gccggcccca cgtgttccag cacgcggggt tcggcgtgga tggccaactg
     1801 tggttgcgac cctaggaccg gggtgcaggg cggcgcttgt gcaaacctca caagtgcgcc
     1861 accgccgcgt tgtgaactca gccatggagc ggcctgcgcc ggtccccgta gcgtcccggc
     1921 gcgtgccagc cgagcgattg ctgccctccg aggaggccac cgccgtggtc gccctggcgc
     1981 gcgattttgc cgcctcggag ctggccccgc gcgcggccag ggccgaggcc gagggccggt
     2041 ttccgcggga tgtcttcgca aggctcggcg agctcgggct gatgggcatg ccctacgagg
     2101 aggagatcgg cggcgggctc tcctacgagg tctacctgca ggtgctggag gagctggcgg
     2161 gggcctgggc gacggtggcg atcggggtca gcgtgcacgt gatgtcctgc ttcgcgctct
     2221 cccgccacgg gtccgcggag gcgcgcgagc gctggctcgg ggagatgctc ggcggcgagc
     2281 tgctcggtgg ctactgcctc tcggaggccg acgccggctc cgacgcggcg gcgatgcgga
     2341 cgcgggcgcg gcgccagggt gagtccttcc ggatcgacgg cagcaaggcc tggatcacgc
     2401 atggcggcga ggccgacttc tacacgctgt tcgcccgcac ctcggatgac cgcacccgcg
     2461 ggatctcctg cttcctggtg cccggcgacg ccgccgggat cagcgctcgg gcgcccgagc
     2521 ggaagatggg cctgaccggg tcggtgaccg cggcgctcga ctgggatggg gtcgaggtcg
     2581 atgccgagcg cctggtcggc gccgaaggcg ccggcctgcc gatcgcactg gaggcgctcg
     2641 acagcggccg gctcgggatc ggcgcgatcg cggtcggggt cgcccagagg gcgctcgacc
     2701 atgccgtcgc ctacgccggc gaacgccatg ccttcggccg gccgatcgcc gagcaccagg
     2761 gcctcggctt cctgctcgcc gacatggccg ccgcggttga gtcggcgcgg gcgacggtgc
     2821 tggaggcggc gcggcggcgc gaccgcgggc tgccgttctc gcgccaggcc tcgatcgcga
     2881 agctggtcgc gaccgacgcg gcgatgtcgg tgacgaccga tgcggtgcag gtgctcggcg
     2941 gctacggcta cacccaggac ttcccggtcg agcggctgat gcgggaggcg aaggcgatgc
     3001 agatcttcga gggcaccaac cagatccagc gcatggtgat cgcccgccag cttggcgatc
     3061 cggcgcgatg aacgacctcg ccggggccgg cgcgacgggg tcgtgatccg gctcggggcg
     3121 ccctgcggat ggcgccgcgt taggcgccgg cgaccacccc ggcgtcgaag aggcggccga
     3181 tctcggcgcc gccgagcccg agcacctcgg cgaggacctg ctcggtgtgc tcgcccagct
     3241 gcggtgcccg gcgcaccggc gctcgctcaa gggtggagaa gtccaggggg ctgcccggca
     3301 tcaggtactc gcccaccccg ggatgggtga ccgcttcgaa catcgggttc tcggtcgaga
     3361 cgcgcgggtc ctcatcgacc agctgacgga acgtctggta gggaccccag gagacgttgg
     3421 tgccggcgaa ggccgcgcgg atcgcggcca gctcgcggga ggcaaaccag gggcgcagca
     3481 gggcggcgat caggtcgcgc ccttcgaagc gcccgctctc gctggcgagg tcgtggccgg
     3541 tggccgcttc gacgccgcgg caggcctcgg cgatcccggt cacctcgacc agcgcctccc
     3601 actggcgggc ggtcagggcg acgaccatca gccggcgccc gtcgcgggtc tcgaagtcgt
     3661 ggccgaaggc gccgtagagg tagttgccgt ccttgggctg gtcggtggcg ccgagggtcg
     3721 cctcggcgat ccggccgagg ttgccgacca tcgcgaaggc gacgtcggag agcgccaggc
     3781 cgatcttctg gccctctccc gtgcgggtgc ggtggcgctc ggcggccagc agcccgaccg
     3841 ccgccagcgt ccccatcgcc acgtcccagg cgggcaggac gctgttcagc ggctcggcca
     3901 ggttgcgcgg gccggtcgcc cagggaaagc cggtcgccgg gttgacggtg tagtcgacct
     3961 cgctggttcc gtcggggttg ccggtcagcg cgaccatgat caggtcggag cggcgctcac
     4021 gcagggtctc gtagccgagc caaccgcggg ccgggaagtt ggtcaggaag agccccgcct
     4081 cgggtcccgg ggcggcgatc agctcggttg ccagctctct gccctcgggg ctgcgcaggt
     4141 cgagcgcgat cgagcgcttg cccttgttca ggcccgccca gaagaggctc tggccgtcgg
     4201 cggccagagg ccagcggccg tggtcgaggc cgccgccgat ctggtcgaag cggatcacct
     4261 cggcgccgag ctgggccagg gtcatgccgc cgagcggggc ggcgacgaag gccgagccct
     4321 cgaccacccg catcccggcg aggatgcctc cgggggcgct catgccagtg ctccgaccag
     4381 gcgccagtcg aggacggcgg cctcctcgcc gtcctcgcgc agcgctcgca cgcgcgagcg
     4441 cagatgggcg agtccgcctc ccgtggccag cggctcgacc cgctccagct cgacctcgct
     4501 gcgcagcatc tcaccctcga agaccgggcc gagatggtcg caggagtgcc aggcgacgat
     4561 cgtcagcagg ccggggatcg ccctggtcag ctgcgcggcg gcgatcccga tcgtgtggcc
     4621 gccgtagacg agccggcgtc cgtgaccggt ggtggcggcg tcgtggtggg cgaaggcgac
     4681 gttgagggag agccgggcga gctccggcgc cgagctgacc aggtcggccc cctcgaccgt
     4741 ccaccgctgg cctgggctga ggtcggcgaa gtggggtccg gggacggcct cgcggtaggc
     4801 ggccaggtcc cagcccgaga gggcggcgcc gagctgctcc gcgctcggct cctcggtccc
     4861 cgtccggatc tcgtcggcat ggccggtctc gccctcgggg tcgcgcagcg gcagcatcgc
     4921 gcagcgttcg aagtcgagca cctcgcgccc ctcctggtca cgggtgcgga ttcgcagcac
     4981 cgccagcccg gttgccgccc gccctggctt ggcggagttc tggcgcaggg cgaccacctc
     5041 ggtggtggtt cgcagggtgt cgccgagccg cggcgcccgc agcagggaga ggccgcggta
     5101 gaagaggttg ccgatcaccc gctgggtgag ctgggtggac tggccgatcg cgacgtcgca
     5161 gaccaggccg ggatgggcga gagccgggcc cggaccgagt acccggctgc tcagctcggc
     5221 gtcgagggcg atgcgcaggc gatctccgag gatcgcctgg tgcagcgctg cgtggccgtc
     5281 ggtgagggtt agcccggggg cgccgtcgag cacctggccg acccgcaggt cctcgaagaa
     5341 gggggcggcg ctgggctcac ccaccgccca gctccgcgag cggcgtgtcg gcggcgacgc
     5401 ggacgatcgc gaggtcgccg gcgatcggct ccgccacgtc ccaggagacg gcatcgaccc
     5461 ttgcgtgggc ggcggcgcgc tccagcggtg ccagcgtccc ggcgtccggc gccggggtcc
     5521 agttggccat cacaaccatg ctcgccccag cgccggcgta agcctcgacg agcccggcga
     5581 gcaggtcggc tgcgacctcg gtgagctcat cgaggtcacc cttcgccggc cggacgtcga
     5641 gcatccgggc ggccatgtcg tggggggagg taacggtcgc aactacgcgg ccagagtacc
     5701 cgggcatccc ggccagcgtg ccgaccagct cgacggcccc actgaccggg ggggtggcag
//
LOCUS       DASRIG010000409         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3585479, whole genome shotgun sequence.
ACCESSION   DASRIG010000409 DASRIG010000000
VERSION     DASRIG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3585479"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VFB52_10960"
     CDS             complement(<1..1087)
                     /locus_tag="VFB52_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO06897.1"
                     /translation="MPVGIDVEGGQGAVREGRGGPELRALPGGRGLDQLVVARRHSPR
                     DSEVRRLLLAGDLIALSLALVVATLLLASRPGEFLWGVLSLPLWVVVFKAYGLYDRDT
                     KRISHGTVDDLPWIFHAMLLGTLMLLALFRLTPAGSIDLANLAAFAVFGTLLVAGLRV
                     AARRLSASLLGPERVLLVGAGPQVETLARKLASHPEYAIEPVAVLAGLDRAELEETIA
                     VERVERVVVAHEDYDDGRLFELLCRCRELGVKLSVLPQLFDALGPSVELDDVEGVTVL
                     GITPPVLPRSSRFLKRAMDVIGAGLLLVLAAPVIALAALAIKLDSRGPVFFRQTRIGR
                     SGRRFELLKLRTMVADAEQRRAELLAQS"
     gene            complement(1092..>2118)
                     /locus_tag="VFB52_10965"
     CDS             complement(1092..>2118)
                     /locus_tag="VFB52_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO06898.1"
                     /translation="WGPNLLRVLAELEETEVSWMCDLDTQRLERFGRRYPAACATTNF
                     EDLLHDPELDAIVIATPVFTHYDLASRALRAGKHTFVEKPLASSSEEAGQLMRLAETV
                     ERRLMCGHTFVYSPPVQAVRSLLAAGELGDIFFISSSRVNLGLHQPDVSVIWDLGPHD
                     FSILLHWLGEVPTKVRAVGRDSIVPGIPDVAFVTLTYASGIVANVELSWLAPSKLRRT
                     VVVGSEKMVVYDDGAPEPVRVFDHGVVYKDPETFGEHQLSYRTGDILSPKIDSSEPLM
                     EELRDFAAAIGSGGEPISSAALARDVVRMIEAADRSLSEGGAEVVLDDEPSEAIFLRS
                     RRQLVAG"
BASE COUNT          329 a          737 c          788 g          264 t
ORIGIN      
        1 tgctctgcgc cagcagctcg gcccggcgct gctcggcgtc ggcgaccatc gtgcgcagct
       61 tgagcagctc gaagcgccgg ccggagcggc cgatccgggt ctggcggaag aagaccgggc
      121 cgcgcgagtc gagcttgatc gcgagcgcgg cgagcgcgat caccggcgcc gccaggacca
      181 gcagcaggcc ggcgccgatc acgtccatcg cccgcttgag gaagcgcgag gagcgcggca
      241 ggaccggcgg cgtgatcccc agcaccgtca cgccctcgac gtcgtcgagc tcgaccgagg
      301 gcccgagcgc gtcgaagagc tgcggcagca cgctcagctt caccccgagc tcgcggcagc
      361 ggcagagcag ttcgaagagg cggccgtcgt cgtagtcctc gtgggcgacg acgacccgct
      421 cgacccgctc tacggcgatc gtctcctcca gctcggcgcg gtcgagaccg gcgaggacgg
      481 cgaccggctc gatcgcgtac tcggggtgcg aggcgagctt gcgggccagc gtctcgacct
      541 gcgggccggc gccgacgagc agcacccgtt cgggcccgag cagcgaggcg gagaggcgcc
      601 gcgccgcgac ccgcagcccg gcgaccagga gcgtgccgaa cacggcgaag gcggcgaggt
      661 tggcgaggtc gatcgagccg gccggggtga gccggaacag cgccagcagc atcagcgtgc
      721 cgagcagcat cgcgtggaag atccagggca ggtcgtcgac ggtgccgtgg ctgatccgct
      781 ttgtgtcgcg gtcatacagc ccgtaggcct tgaagacgac aacccagagc ggcagcgaga
      841 gcacccccca gaggaactcg ccgggacggc tggcgagcag cagcgtcgcg acgaccaggg
      901 cgaggctgag cgcgatcagg tcaccggcga gcagcagccg ccggacctcg gagtcgcgcg
      961 ggctgtgacg gcgggcgacg accagctggt cgagcccgcg gccgccgggc agcgcccgca
     1021 gctcggggcc gccgcggccc tcgcggaccg cgccctgacc gccctcgacg tcgatcccga
     1081 ccggcatctc gctacccggc gacgagctgg cggcgcgagc gcaggaagat cgcttcgctc
     1141 ggctcgtcgt cgagcacgac ctcggcgccg ccttccgaca gcgagcggtc cgcggcctcg
     1201 atcatgcgga cgacgtcgcg ggcgagcgcg gcgctggaga tcggctcgcc gccgctgccg
     1261 atcgccgcgg cgaagtcgcg cagctcttcc atcagcggct cgctgctgtc gatcttcggc
     1321 gagaggatgt cgccggtccg gtaggagagc tggtgctcgc cgaaggtctc ggggtctttg
     1381 tagacgacgc cgtggtcgaa gacccgcacc ggctccggcg cgccgtcgtc gtagacgacc
     1441 atcttctcgc tgccgacgac gaccgtgcgc cgcagcttgc tcggcgccag ccagctgagc
     1501 tcgacgttgg cgacgatccc gctcgcgtag gtgagggtga cgaaggcgac gtcggggatc
     1561 ccgggcacga tcgagtcgcg gccgaccgcg cgcaccttgg tcgggacctc ccccagccag
     1621 tgcaggagga tcgagaagtc gtgcgggccg aggtcccaga tcacgctgac gtcgggctgg
     1681 tgcagaccga ggttcacacg gctggaggag atgaagaaga tgtcacccag ctcgcctgcg
     1741 gcgagcagag accggacggc ctgcaccggc ggactgtaga cgaaggtgtg cccacacatc
     1801 agccgccgct cgaccgtctc ggccagccgc atcagctgcc cggcctcctc cgaggacgag
     1861 gccaacggct tctcgacgaa cgtgtgcttg ccggcgcgca gcgcccgcga ggcgaggtcg
     1921 tagtgggtga agaccggggt cgcgatcacg atcgcgtcga gctcggggtc gtggagcagg
     1981 tcctcgaagt tggtggtcgc acaggcggcc gggtagcggc ggccgaagcg ctcgagccgc
     2041 tgggtgtcga ggtcgcacat ccagctgacc tcggtctcct ccagctcggc cagcacccgc
     2101 agcaggttcg gcccccag
//
LOCUS       DASRIG010000410         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3302514, whole genome shotgun sequence.
ACCESSION   DASRIG010000410 DASRIG010000000
VERSION     DASRIG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302514"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFB52_10970"
     CDS             complement(<1..391)
                     /locus_tag="VFB52_10970"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZO06899.1"
                     /translation="MAQPRALITGITGQDGSYLAELLLAKGYEVHGLVRRLSSENYYR
                     LAGIQDRISLHGGDLLDQRSLVEAMRKSEPDEIYNLAAMSFVAASWSQPTLTAQFTGV
                     GVVHMLEAMRETCPGARFYQASSSEMFG"
     gene            complement(479..1429)
                     /locus_tag="VFB52_10975"
     CDS             complement(479..1429)
                     /locus_tag="VFB52_10975"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZO06900.1"
                     /translation="MGRRAALITGITGQDGSYLAELLLDKGYEVHGLVRGGAAERDLG
                     PLTGRVVAHRGELGDGGSLLAALRESEPDEVYNLAAVSHVPASSDAVTASADVNALGV
                     GRLLAAIVKCGSRARFFQASSSEMFPHSAGRPQDEATPLAPRSAYGVAKAYGHLLTAD
                     YRRHGLFACSGILFNHESPRRGPQFVSRRVVQGAVAIKLGRSAELALGNLDARRDWGY
                     APEYVEAMWLMLQAEDPEDYVIGSGHEHSVAELVAAAFGGLDLEPERYLRLDPAAARE
                     HDLEYALADPTKARERLGWSARTGFDELVRIMVAAELAAD"
     gene            complement(1408..2163)
                     /locus_tag="VFB52_10980"
     CDS             complement(1408..2163)
                     /locus_tag="VFB52_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO06901.1"
                     /translation="MKLSVVMPAYNEEGSVGRTVDGVVAALERTEIDYEVVVVNDNSE
                     DSTEAIVNAIGAANPRVRCHRSHYEGGFGMAIRAGLDVYEGDAVVIVMADASDNPEDL
                     VRYHRLLEDGWDCAFGSRFVPGAKVYDYPRLKYYVNRLANQFIRVLFRHRYNDTTNAF
                     KAYRREVIETIQPLLSKHFNLTVEMPLKAIVRGHSYEVIPTDWTNRETGVAKLKMKEM
                     GSRYLFITLYVWLEMTLSRGDYRVRKPWAGARR"
     gene            complement(2160..3230)
                     /locus_tag="VFB52_10985"
     CDS             complement(2160..3230)
                     /locus_tag="VFB52_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO06902.1"
                     /translation="MPVAIITGAGGLIGSEAVEHFIAQGFDVVGIENDMRARFFGPES
                     STSHVTERLVAEHSSFHWENADIRDTEAIERIFAKHAGQIELVIHTAAQPSHDWAASE
                     PQTDFGVNANGTLNLLEAARAHCLEAPFIFCSTNKVYGDTPNRLPLQSLEKRLELPED
                     HPYYKGIDTTMSIDSSTHSLFGVSKAAADLLVQEYGYYFEMPTVCFRGGCLTGPQHAG
                     AKLHGFLAYLMKCTVTGTPYTVFGYEGKQVRDNIHSADLIAAFDAFRKAPRAHAVYNI
                     GGGRFSNCSMLEAIDVCERVSGRELSWEMGEEPRIGDHRWWISDLAPFEADYPEFKLR
                     YGIEEILQEMYEQNLERWTVPA"
     gene            complement(3260..>3726)
                     /locus_tag="VFB52_10990"
     CDS             complement(3260..>3726)
                     /locus_tag="VFB52_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO06903.1"
                     /translation="IAGPWQMGKVDQGVFTHWMLAHHFGNPLSYIGFGGAGKQVRDLL
                     HVEDLVDLVERQLLAPEDWNGRTVNVGGGRECSLSLQETTALCRELSGHEVPIEPVLE
                     TRQGDVPIYLSDCERLFGLDQWRPRRSARDVLTDIHEWVAADPERIAAALNV"
BASE COUNT          562 a         1231 c         1321 g          612 t
ORIGIN      
        1 ggccgaacat ctcgctcgag gaagcttggt agaagcgggc accgggacag gtctcgcgca
       61 tcgcctcgag catgtggacc acaccgacac cggtgaactg ggcggtcagg gtcggctggc
      121 tccaggaggc ggcgacgaac gacatcgccg ccaggttgta gatctcgtcg ggctcgctct
      181 tgcgcatcgc ctcgaccagc gagcgctggt cgagaagatc gccgccgtgc aggctgatcc
      241 ggtcctggat tccggccagg cggtaatagt tctcgctcga aagccggcgc accaggccgt
      301 gcacctcgta cccctttgcc agcagcagct cggcgaggta gctgccgtcc tggccggtga
      361 tgccggtgat cagcgcgcga ggttgggcca ctttgccagc gtacccccga aaaccctgca
      421 ctcacgcaag attggacact tcgactagtg gtcgccgacg gagatccggc cgggggcgct
      481 aatcggcggc cagctcggcg gcaaccatga tccgcaccag ctcgtcgaac ccggttcgcg
      541 cgctccagcc gagccgctcg cgcgccttcg tggggtcggc gagggcgtac tcgaggtcgt
      601 gttcccgggc ggccgcgggg tcgaggcgca gataccgctc gggctcgaga tcgagcccgc
      661 cgaaggcggc tgcgaccagc tctgcgaccg agtgctcgtg accgctgccg atcacgtagt
      721 cctcggggtc ctcggcctgg agcatcagcc acatcgcttc cacgtactcg ggtgcgtagc
      781 cccagtcgcg gcgcgcatcg aggttgccga gcgccagctc ggcggagcgg ccgagcttga
      841 tcgcgaccgc gccctgcacg acccggcggc tgacgaactg cggaccgcgc cgcgggctct
      901 cgtggttgaa gaggatgccg gagcaggcga agaggccgtg gcggcgatag tcggcggtca
      961 gcaagtggcc gtaggccttc gccaccccgt aggcagagcg cggcgccagc ggcgtcgcct
     1021 cgtcctgggg acgcccagcg ctgtgcggga acatctcgct cgaggaggcc tggaagaagc
     1081 gcgcccgcga gccgcacttg acgatcgccg cgagcaaccg cccgaccccg agcgcgttga
     1141 cgtcggcgct cgcggtcacc gcgtccgagg aggccgggac gtgcgagacg gcggcgaggt
     1201 tgtagacctc gtcgggttcg ctctcgcgca gcgccgccag cagcgagccg ccgtcgccga
     1261 gctcaccgcg gtgggcgacg acccgcccgg tcagcgggcc gaggtcgcgc tctgccgcgc
     1321 cgcctcgcac gaggccgtgc acctcgtagc ccttgtccag cagcagctcc gccagatagc
     1381 tgccgtcctg cccggtgatc ccggtgatca gcgccgcgcg ccggcccacg gtttgcggac
     1441 gcggtagtcg ccgcggctga gcgtcatctc gagccagacg tagagggtga tgaagaggta
     1501 gcggctgccc atctccttca tcttcagctt cgccacgccg gtctcgcggt tggtccagtc
     1561 ggtcgggatc acctcgtagg agtgcccgcg gacgatcgcc ttcagcggca tctcgacggt
     1621 caggttgaag tgcttggaga gcaatggctg gatcgtctcg atcacctcgc gccggtacgc
     1681 cttgaaggcg ttggtggtgt cgttgtagcg gtggcggaag aggacgcgga tgaactggtt
     1741 ggcgaggcga ttgacgtagt acttgagccg cgggtagtcg taaaccttgg cgccgggcac
     1801 gaagcgggag ccgaaggcgc agtcccagcc gtcttcgagc aggcggtggt agcggaccag
     1861 gtcctcgggg ttgtcggagg cgtcggccat cacgatcacg accgcgtcgc cctcgtagac
     1921 gtcgaggcca gcgcggatcg ccatcccgaa gccaccctcg tagtgggagc ggtggcagcg
     1981 cacccgcggg ttcgcggcgc cgatcgcgtt gacgatcgct tcggtcgagt cttctgagtt
     2041 gtcgttgacg acgacgacct cgtagtcgat ctctgtgcgc tccagggccg cgaccacgcc
     2101 gtcgaccgtg cgcccgaccg agccctcctc gttgtaggcg ggcatcacta cggagagctt
     2161 catgccggaa cggtccagcg ctcgaggttc tgctcgtaca tctcctggag gatctcctcg
     2221 atcccgtagc gcagcttgaa ctccgggtag tcggcttcga acggcgccag gtcggagatc
     2281 caccagcggt ggtcgccgat ccgcggctcc tcgcccatct cccaggagag ctcacgcccg
     2341 gagacccgct cgcagacgtc gatcgcctcc agcatcgagc agttggagaa gcgcccgccg
     2401 ccgatgttgt agacggcgtg ggcgcgcggc gccttgcgga aggcgtcgaa ggcggcgatc
     2461 aggtcagcgc tgtggatgtt gtcgcgcacc tgtttgccct cgtagccgaa caccgtgtag
     2521 ggcgtgccgg tcacggtgca cttcatcaag taggcgagga agccgtgcag cttcgcgccg
     2581 gcgtgctggg ggccggtcag acagccgccg cggaagcaga cggtcggcat ctcgaagtag
     2641 tagccgtact cctgcaccag caggtcggcg gccgccttgg agaccccgaa cagcgagtgg
     2701 gtcgagctgt cgatcgacat cgtcgtgtcg atccccttgt agtaggggtg atcctcgggc
     2761 agctccagcc gcttctccag cgactgcagg ggcaggcggt tgggcgtgtc gccgtagacc
     2821 ttgttggtcg agcagaagat gaacggcgcc tcaaggcagt gcgcgcgcgc agcctcgagc
     2881 aggttgaggg tgccgttggc gttgacgccg aagtcggtct gcggctcgct cgcggcccag
     2941 tcgtgcgagg gctgggcggc ggtgtggatc accagctcta tctggccggc gtgcttggcg
     3001 aagatccgct cgatcgcctc ggtgtcgcgg atgtcggcgt tctcccagtg gaaggaggag
     3061 tgctcggcga cgaggcgctc ggtcacgtgc gaggtcgagg actcgggacc gaagaagcgc
     3121 gcccgcatgt cgttctcgat gccgaccacg tcgaagccct gggcgatgaa gtgctcgacg
     3181 gcctcggacc cgatcaggcc ccccgccccc gtgatgatcg caaccggcat ctggctctgt
     3241 gtctccttct ggtctcggac tagacgttca gcgcggcggc gatccgctcg ggatcggcag
     3301 ccacccactc gtgtatatcg gtcagcacgt ctcgcgcgct tcgccgcggc cgccactggt
     3361 cgaggccgaa cagccgctcg cagtcggaga ggtagatcgg gacgtcgccc tggcgcgtct
     3421 ccaggaccgg ctcgatcggc acctcgtggc cgctcagctc gcggcagagc gcggtcgtct
     3481 cctgcagcga gagcgagcac tcgcggccgc cgccgacgtt gaccgtgcgt ccgttccagt
     3541 cttccggcgc cagcagctgg cgctcgacca ggtcgaccag gtcctcgacg tggagcaggt
     3601 cgcggacctg cttgccggcg ccgccgaagc cgatgtagga gagcgggttg ccgaagtggt
     3661 gggcgagcat ccagtgggtg aagaccccct ggtcgacctt gcccatctgc caggggccgg
     3721 cgatca
//
LOCUS       DASRIG010000411         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_965709, whole genome shotgun sequence.
ACCESSION   DASRIG010000411 DASRIG010000000
VERSION     DASRIG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965709"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /gene="mrdA"
                     /locus_tag="VFB52_10995"
     CDS             <1..1973
                     /gene="mrdA"
                     /locus_tag="VFB52_10995"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZO06904.1"
                     /translation="RCAVMPGRGDWRGPPIPAQLAFRVTVLGGVALVMFAIIFLRLWY
                     LEVLSGDQYLAEAQNNQVREFTVQAPRGEILDRSGRPLVQNRTALELQLKTTELPRDR
                     ERRARLFKRVGELAGMSQKQIRRQIRVQHKELPASPVTLKRDVAFDTVYYIRENQRRF
                     PGVSVERVYVRRYPQGSLAAHLLGYVREISPEELKDPRYESLEQGDYVGKQGVEYTYD
                     SLLRGVNGASRVQVDAAGEPTGRSLDVREPRTGNDLVLTLDEGVQSAGESAIGQFGLP
                     GGFVAMNVNDGEILGMGSTPTYDPSLFAQPVVTPATYKQIFGDPKDPLSTLAAPAVNR
                     ASQGVYPTGSTFKPITAVAALDAGNLTPERTIADGGSVEYGGVKFENAGGVAHGTVSL
                     QTALQVSSDVFFYIMGAETNVQEGDGGAIQEWARALGLGAPTGLDVGGEATGRVPTPE
                     ERNEAFQENTDPDSPSGKDVDVAKGELTDRPWSIGDNINLAVGQGDLQADPLQMAVAY
                     AAIANGGTVVRPHVGLRIEDPQGRAIQEIDPAPRRELDIDPIWQQTIMAGLHDAAMAP
                     GGTSYPIFGGYPVDIAGKTGTAERPPYGDQSWYVAVAPYDDPKYVVAVTIEEGGFGAD
                     AAAPATLEILNELLDVNTAKIDSVSADVAVVE"
BASE COUNT          336 a          728 c          759 g          296 t
ORIGIN      
        1 ggaggtgcgc cgtgatgccg ggccgcggcg actggcgcgg gccgcccatc ccggcgcagc
       61 tcgccttccg ggtcaccgtg ctcggcggag tcgcgcttgt catgttcgcg atcatcttcc
      121 tgcggctctg gtacctcgag gtgctctccg gcgaccaata cctcgccgag gcgcagaaca
      181 accaggtgcg ggagttcacc gttcaggccc ctcgcggcga gatcctcgac cggtcagggc
      241 gcccgctggt tcagaaccgg accgcgctcg agctgcagct caagacgacc gagttgccgc
      301 gcgaccggga gcgccgagcg cggctgttca agcgggtcgg tgagctggcg ggcatgtcgc
      361 agaagcagat ccgccgccag atccgggtgc aacacaagga gctgccggcg agcccggtga
      421 cgctcaagcg cgacgtcgcc ttcgacaccg tctactacat ccgcgagaac cagcggcgct
      481 tcccgggtgt ctccgtggaa cgcgtctacg tccggcgcta tccgcagggg agcctggccg
      541 cccatctcct cggctacgtg cgcgagatct cccccgaaga gctcaaggac ccccgctacg
      601 agagcctgga gcagggcgac tacgtcggga agcagggggt cgagtacacc tacgacagcc
      661 tgctgcgcgg cgtcaacgga gcctcgcggg tgcaggtcga cgccgccggg gagccgaccg
      721 gccgcagcct cgacgtgcgc gagccgcgca ccggcaacga cctcgtgctg acgctcgacg
      781 agggggtcca gagcgccggg gagtcggcga tcgggcagtt cgggctgccg ggcgggttcg
      841 ttgcgatgaa cgtcaacgac ggcgagatcc tcggcatggg gtcgacgccg acctacgacc
      901 cgtcgttgtt cgcgcagccg gtcgtcaccc cggctaccta caagcagatc ttcggcgacc
      961 ccaaggaccc gctgagcacg ctcgccgcgc cggcggtgaa ccgcgcctcg cagggcgtct
     1021 atcccaccgg ctcgacgttc aaaccgatca ccgccgtggc ggcgttggac gccggcaacc
     1081 tgaccccgga gcggacgatc gctgacggcg gctcggtcga gtacggcggg gtgaagttcg
     1141 agaacgcggg cggtgtcgct cacggcacgg tcagcctgca gaccgcgctg caggtctcgt
     1201 cggacgtgtt cttctacatc atgggcgcgg aaaccaacgt ccaggagggc gacggcgggg
     1261 cgatccagga atgggcccgg gcgctgggcc tcggggcccc gacgggcctc gacgtcggcg
     1321 gcgaggcgac cgggcgggtg ccgactcccg aggagcgcaa cgaggccttc caggagaaca
     1381 ccgaccccga ctcgcccagc ggcaaggatg tcgacgttgc caagggcgag ctcaccgacc
     1441 gtccgtggtc gatcggcgac aacatcaacc tcgccgtggg tcagggcgat ctgcaggccg
     1501 acccgctgca gatggcggtc gcctacgcgg cgatcgccaa cggcggcacg gtcgtgcgcc
     1561 cgcacgtagg cctgcggatc gaggatcccc agggtcgtgc gatccaggag atcgaccccg
     1621 ctccacgccg cgagctcgac atcgacccga tctggcagca gacgatcatg gcgggactgc
     1681 acgacgccgc gatggcgccg ggcgggacct cgtatccgat cttcggcggt tatccggtcg
     1741 acatcgcggg caagaccggc accgccgagc gcccgccgta cggggaccag tcctggtacg
     1801 tggcggtggc gccgtatgac gatcccaagt acgtggtggc cgtgacgatc gaggaggggg
     1861 gcttcggggc cgacgccgcg gcgcccgcga ccctggagat cctcaacgag ctgctcgatg
     1921 tgaatacggc gaagatcgac agcgtctcgg cagacgtagc ggtggtggag tgaggcggag
     1981 cggagcgaag ccgagccgcg ccgctgttga ggcgcctgtc tgatggaggc ctttccccgc
     2041 ccacggccct tcgagcagcc tcgcccgtcg ctgctgtcgc ggatcgggtt cggcaacctc
     2101 gacccgctgc tgctcgtcg
//
LOCUS       DASRIG010000412         6560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1508522, whole genome shotgun sequence.
ACCESSION   DASRIG010000412 DASRIG010000000
VERSION     DASRIG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508522"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..640)
                     /locus_tag="VFB52_11000"
     CDS             complement(35..640)
                     /locus_tag="VFB52_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZO06905.1"
                     /translation="MNQAATAAPPTPDATAPAVGPRIRALRDAMGLSLRDLAERSGVS
                     AQMLSQVERGGTSPTLASATKIAAGLELTLSQLLRLDEGQHVAVSRASGRRRYERGGH
                     RFEELTPPMPGQRADVSLHVLEPGATTGGRTDPPMHEPGSRETAVVTAGTLALVVDGS
                     RYDLQEGDSVTFDADLPHHFENDGEDPTEFFAVIAAGLRRG"
     gene            complement(654..1463)
                     /locus_tag="VFB52_11005"
     CDS             complement(654..1463)
                     /locus_tag="VFB52_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZO06906.1"
                     /translation="MGARIAGLAEAQHGVVTRAQLRELSLSDDAINRRKRSGGLHPLY
                     PGVFAVGHRVLSRESHWLAAVLVCGPGAVLSFRSAAAHWGIRGYSGAYVDITSPSKTR
                     SRGSIRRHCARLLADEVAEHEGIPITSASRTIFDLSATLVPQDLEAALRQSEYLRLYS
                     SLSLWDLLERYPRHRGSRSIRSALARLEEAPGEVYEGLEERFLAFLDAHHLPRPHLNA
                     WLEVQGHRYKADCLWPEQKLIAELDSWQMLDHEAELLAADLLALLAHDKRP"
     gene            complement(1528..1656)
                     /locus_tag="VFB52_11010"
     CDS             complement(1528..1656)
                     /locus_tag="VFB52_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06907.1"
                     /translation="MTLPLAHHAAVAALPVFAPALIIVSVLTIRFLRERRHWDDES"
     gene            complement(1653..3479)
                     /locus_tag="VFB52_11015"
     CDS             complement(1653..3479)
                     /locus_tag="VFB52_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06908.1"
                     /translation="MDITLIAQTSQAAPPAGGAELFQIALASVAAAFLTVALLYVGFG
                     HRSGRVAILGRLAADAERRTGLPGWAALPAGIATVSLLTALFGMYWDISLHIDNGRDA
                     GPLANPAHYFILTGLFGIFTAGFIAMVLPQGRPSTAAIRLGRDWWAPLGGVLICACGA
                     FSLIGFPLDDLWHRLFGQDVTLWGPTHLMLIGGAAMTLVGIAVLTIEGQRANESAGHA
                     DAEHGSTRALRAVALTGGLMLGLSTFQAEFDFGVPQFRMVFQPLLLMLAAGVGLVLAR
                     IYGGRGAALGAAGFFIVLRGALALAIGPGLGQSVPHFPLYIVEALVVEGVALAVSTKR
                     PLRFGLWAGLGIGTVGLAAEWAWSHIWMPLPWPSALFPEGAVIGFLAAMAGAVLGAWI
                     GAHLTVEPGVRRPALRQLAVGAAALTAALIALALYTPTQRGVRADVVLRPLGEGEVQA
                     TLTMHPPDAAAEAEWFDVTAWQGGGLVVAPLEEIAPGRYRTSEPIPVDGNWKSMVRLH
                     EGRSLTALPIFLPRDPAIPVGEVPAPPRFTRAFGDEHQLLQREQKGGSPLVVTLAYST
                     VAAITLSLLALIAWALHRLSFGPATRRRPAAHSLDPSESAAR"
     gene            3547..4320
                     /locus_tag="VFB52_11020"
     CDS             3547..4320
                     /locus_tag="VFB52_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO06909.1"
                     /translation="MAKRTQAAEAPSASPEFEATLTVEQLAHETGMSVRNIRNHQSRG
                     LLQPPEVRARIGYYGPAHVARLRLIQEMQAEGFKLSAISRLIGEHGDNADRFVGLRQA
                     VTAPFATEAPEVFSRTDLIERFGVDDEKLLAKAQKLGVLVDLGEERFEAPSPSLLRAA
                     EEVLTMGIPLPAALAAIEKLSRNSQSSARAFVNLFVEELWKPFDEAGRPDEGWAEITA
                     AIERLRPLAVEAENAAFRLALTAEIEKAFGEVLERKKRK"
     gene            4353..5354
                     /locus_tag="VFB52_11025"
     CDS             4353..5354
                     /locus_tag="VFB52_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZO06910.1"
                     /translation="MSTTDFAEVGAERGSPVGVAPSGGFQHWSAVRRIGLAIYALVIV
                     LWSARYGIPVQRELVIAWVCGALAIFSIGRPPRQLLQLVLEWMPIVLVLIAYDYTRGA
                     ADTFGIPVNYTEMIDFDKAVFFGQTPTEWLQAHLYEPNVVHWYDVAFTLIYTSYFITP
                     FAVLGYLWARDHPAFVRLAKRIVTLALLGLATYILYPAAPPWMAGEMGLLDEVHRTTA
                     KGWEVVGIGTASWFSKGQGLGNPVAAVPSLHTAYCALVVLFLWSRVRRRWLRPLLLLY
                     PLAMGLTLMATGEHYFFDVFLGFVYAGGIMAGWGWWERRRASARAGTGAALEQGNET"
     gene            complement(5304..>6560)
                     /locus_tag="VFB52_11030"
     CDS             complement(5304..>6560)
                     /locus_tag="VFB52_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZO06911.1"
                     /translation="GGERLVFGAPERLLDGAATAVALRERAAANAEDGRRVLALAAAD
                     GPLPAASPTAELPAALRPLGIVVLAEELRANATDTVSFFAAEEVTLRVLSGDNPATVG
                     AIARDVGIEARDDALDGGALPEGDEELLAAVRAAPAIGRISPEDKARVVRVLAEGGEY
                     VGMLGDGVNDVPALKQARLAIAQGSGTQMARSVSDLVLVSGEFGEVPPMVHEGRQILR
                     NIQRVARLFVSKAIFTAFLLVTIALTSGVFPLLPRQFTLTSSLTIGIPAFFLALAPST
                     GPWRPEGFLKAIARFSIPAGIACGLGILVSYLVARHGFDVDLEEARTVTAATVVTVGL
                     AIVIALEDEPGMRRYAVLGLCALMALGFVLVAAIPAGRDFFELATPTGDMLAAWAIGA
                     ATAIVLLLASLRLVALLERRAGPGPG"
BASE COUNT         1004 a         2200 c         2458 g          898 t
ORIGIN      
        1 gtcttcctct ctcgcttccg cgtccggggg cggcttaacc ccgcctcagc cccgcagcga
       61 ttacggcgaa gaactcggtg gggtcctcgc catcgttctc gaaatgatgg ggcaagtcgg
      121 cgtcgaaggt gacgctgtcg ccctcctgca agtcgtagcg gctgccgtcg accacaagcg
      181 ccagggtgcc ggcggtgacc acggcggtct cgcggctgcc cggctcgtgc atcggcgggt
      241 cggtgcggcc accggtggtg gcgccgggct ccagcacgtg cagggagacg tcggcgcgct
      301 ggcccggcat cggcggcgtc agctcctcga accggtgccc cccacgctcg tagcggcgac
      361 ggccggaggc gcggctgacg gcgacgtgct ggccctcgtc gaggcgcagc aattgcgaga
      421 gcgtcagctc gaggccggcg gcgatcttcg tcgcggaggc cagcgtcggg ctggtcccac
      481 cgcgctcgac ctgcgagagc atctgcgcgc ttaccccgga gcgctccgcg aggtcgcgca
      541 gggacaagcc catggcgtca cgcagggcgc ggatgcgggg gccgacggcg ggggcggttg
      601 cgtcgggggt gggtggtgcg gcggtggcag cttggttcac gggatgtacg ttatcacgga
      661 cgtttgtcat gtgccagcag agccaggagg tcggcggcga gtagctcggc ttcgtggtcg
      721 agcatctgcc aggagtcgag ctcggcgatc agcttctgtt caggccagag gcagtcggcc
      781 ttgtagcggt ggccttggac ttccagccag gcgtttaggt ggggacgagg caggtgatgg
      841 gcatcgagga aggcgaggaa gcgctcctcg agaccctcgt agacctcgcc gggcgcttct
      901 tcgagcctgg cgagggcgct gcggatcgat ctactcccac ggtggcgtgg atatctctcc
      961 aacagatccc agagggagag ggaggaatag agccgcaggt attcagattg acggagagcg
     1021 gcctcaaggt cctggggtac cagggttgcc gagaggtcga agatggtgcg ggaggccgag
     1081 gtgatcggga tcccctcgtg ctcagccact tcgtccgcca gcaagcgagc gcagtggcgg
     1141 cggatcgagc cccgagacct cgttttactg ggcgaggtga tgtcgacgta ggcgcccgag
     1201 tagccccgaa tgccccagtg ggctgcagcg gagcggaagc tcagtaccgc tccgggaccg
     1261 cagacgagca ccgccgccag ccagtgcgac tctcgcgaga ggactctgtg gccgacggca
     1321 aacacaccgg gatagagcgg atgcaggccc ccggatcgct ttcgacggtt gatcgcgtcg
     1381 tcactgaggc tcagttcccg gagctgcgcc cgcgtgacca ctccgtgctg tgcctcggcc
     1441 aggccggcga tcctggcgcc catttcacga gtccgttctg atcggcgcat atcgccgata
     1501 aggacggact cgtggtgggg tctcccccta ggactcgtcg tcccagtggc ggcgttcgcg
     1561 gaggaagcgg atggtgagga cggagacgat gatcagggcg ggggcgaaga cagggagggc
     1621 ggcgactgcg gcgtggtggg cgagggggag ggtcatcggg ctgcgctttc gcttgggtcg
     1681 agggagtggg ccgccgggcg gcggcgggtg gcggggccga aagagaggcg gtggagggcc
     1741 caggcgatca gggccagcag ggagagggtg atcgcggcga cggtcgagta ggcgagggtg
     1801 acaaccagag ggctgccgcc tttctgctcg cgctggagga gctggtgctc gtcgccgaag
     1861 gcgcgggtga aacggggcgg ggcggggact tcgccgaccg ggatcgccgg gtcgcggggg
     1921 aggaagatcg gcagggcggt gagcgagcgg ccctcgtgca ggcgcaccat cgacttccag
     1981 ttgccgtcga ccgggatcgg ctcggaggtc cgatagcggc ccggggcgat ctcctcgagg
     2041 ggggcgacga cgaggccgcc gccctgccag gcggtgacgt cgaaccactc cgcctcggcg
     2101 gcagcatcgg gcgggtgcat ggtcagggtc gcctgcacct cgccttcgcc gagcgggcgc
     2161 aggacgacat cggccctcac tccgcgctgg gtcggcgtgt acagagccaa tgcgatcagg
     2221 gccgcggtca gggcagcagc cccaaccgcc agctggcgca acgccggccg ccgcacgcct
     2281 ggctcgaccg tcaggtgggc gccgatccag gcgccgagca cggccccggc catcgcggcg
     2341 aggaagccga tcacggcgcc ctccgggaag agcgcgctcg gccagggcag cggcatccag
     2401 atgtgcgacc aggcccactc ggcggcgagg ccgacggtgc cgatcccgag cccggcccag
     2461 aggccgaagc gcagtgggcg cttggtcgag accgcgagcg ccacgccctc gaccaccagc
     2521 gcctcgacga tgtagagcgg gaagtggggg acggactggc cgaggccggg accgatcgcc
     2581 agcgccagcg cgccgcgtag gacgatgaag aagccggcgg cgccgagggc cgcgccgcga
     2641 cccccatata tacgggcgag gaccagcccg accccggccg ccagcatcag cagcagcggc
     2701 tggaagacca tccggaactg gggcacgccg aagtcgaact cggcctggaa ggtggagagg
     2761 ccgagcatca ggccgccggt cagggcgacc gcgcgcaggg cgcgcgtgga accgtgctcg
     2821 gcgtccgcgt ggcccgcgga ctcgttggcg cgctgcccct cgatcgtcag cacggcgatc
     2881 ccgaccaggg tcatcgcggc gccgccgatc agcatcaggt gggtcgggcc ccagagggtc
     2941 acgtcctgcc cgaacagccg gtgccagagg tcgtcgagcg gaaagccgat cagcgagaag
     3001 gcgccgcagg cgcagatcag cacgccgccg agcggcgccc accagtcgcg gccgaggcgg
     3061 atcgcggcgg tgctcggccg gccctgcgga agcaccatcg cgatgaaccc ggcggtgaag
     3121 atcccgaaga ggccggtgag gatgaagtag tgggccgggt tggcgagcgg cccggcgtcg
     3181 cggccgttgt cgatgtgcag ggagatgtcc cagtacatgc cgaacagcgc ggtgagcagg
     3241 gagacggtgg cgatcccggc cggcagcgcg gcccagccgg gcaggccggt gcggcgttcg
     3301 gcgtcggcgg cgagccggcc gaggatggcg acgcggccgg agcggtggcc gaagccgacg
     3361 tagagcaggg cgacggtgag gaaggccgcg gcgacggacg cgagcgcgat ctggaagagc
     3421 tcggcgccac cggcgggagg agcggcctgc gaagtctgtg cgatcagggt tatgtccatg
     3481 gcctccacca ttacctcgtt tgacgttacc tcaaacgagg ttacattcaa aatgtaagat
     3541 cgatgtgtgg ctaagcgcac gcaagccgcg gaggccccct ccgcgagccc tgagttcgag
     3601 gcgacgctga ccgtcgagca gctcgcccac gagaccggga tgtcggtgcg caacatccgc
     3661 aaccaccagt cacgcggcct gctgcagccg cccgaggtcc gcgcccggat cggttactac
     3721 ggccccgccc acgtcgcccg cctgcgcctg atccaggaga tgcaggcgga gggcttcaag
     3781 ctgagcgcga tctcacggct gatcggcgag cacggcgaca acgccgaccg cttcgtcggc
     3841 ctgcgccagg cggtcaccgc accgttcgcg accgaggcgc cggaagtctt cagccgcacc
     3901 gacctgatcg agcgcttcgg ggtcgacgac gagaagctgc tggcgaaagc gcagaagctc
     3961 ggcgtgctcg tcgacctcgg cgaagagcgc ttcgaagcgc ccagcccttc cctgctgcgc
     4021 gctgccgaag aggtgctgac gatgggcatc ccgctgccgg cggcgctggc ggcgatcgag
     4081 aagctgagcc gcaactcgca gtcctcggcg cgcgccttcg tcaacctctt cgtcgaggag
     4141 ctgtggaagc cgttcgacga ggccggccgg cccgacgagg gctgggccga gatcaccgcc
     4201 gcgatcgagc gcctgcgccc gctcgcggtc gaggccgaga acgccgcttt ccgcctcgcc
     4261 ctcaccgccg agatcgagaa ggccttcggc gaagttctcg aacgcaagaa gcgcaagtaa
     4321 cgatccagtt acccggcggt taaattggtc gcttgagcac gaccgatttc gcagaggtcg
     4381 gggccgagcg ggggtccccc gttggagtgg cgccgtccgg cggcttccag cactggtccg
     4441 cggtgcgccg catcgggctg gcgatctacg cgctggtgat cgtcctctgg agcgctcgct
     4501 acgggatccc ggtccagcgc gagctggtga tcgcctgggt ctgcggcgcg ctcgcgatct
     4561 tctcgatcgg ccggccgccg cgtcagctgc tgcagctcgt gctcgagtgg atgccgatcg
     4621 tcctcgtcct gatcgcctac gactacactc gcggcgccgc cgacaccttc ggcatccccg
     4681 tcaactacac ggagatgatc gacttcgaca aagcggtctt cttcggccag acgccgaccg
     4741 aatggctgca ggctcacctc tacgagccca acgtcgtgca ctggtacgac gtcgccttca
     4801 ccctgatcta cacctcgtac ttcatcactc ccttcgcggt cctcggctac ctctgggccc
     4861 gcgaccaccc ggctttcgtc cgcctcgcca agcgcatcgt caccctggcg ctgctcggcc
     4921 tcgccaccta catcctctac ccggccgcac cgccctggat ggccggcgaa atgggcctgc
     4981 tcgacgaagt ccaccggacc accgcgaagg gctgggaagt ggtcggcatc ggcaccgcca
     5041 gctggttctc caagggccag gggctcggca acccggtcgc cgcggtgccc tcgctgcaca
     5101 ccgcctactg cgccctggtc gtcctcttcc tctggtcgcg ggtgcggcgg cgctggctgc
     5161 ggccgctgct gctcctctat ccgctggcga tgggcctgac cctgatggcg accggcgagc
     5221 actacttctt cgacgtcttc ctcggcttcg tctacgccgg cgggatcatg gccggctggg
     5281 gctggtggga acggcgccgg gcctcagccc gggccgggac cggcgcggcg ctcgagcagg
     5341 gcaacgagac gtagggacgc gagcagcagg acgatcgcgg tggcggcgcc gatcgcccag
     5401 gcggcgagca tgtcgccggt cggggtcgcc agttcgaaga agtcgcggcc ggcggggatc
     5461 gcggcgacga gcacgaagcc gagcgccatc agcgcgcaga ggccgagcac ggcgtagcgg
     5521 cgcatgcccg gctcgtcctc gagcgcgatc acgatcgcga ggccgaccgt gaccacggtc
     5581 gcggcggtca ccgtgcgcgc ctcctcgagg tcgacgtcga agccgtggcg ggcgaccagg
     5641 tagctgacca ggatgccgag cccgcaggcg attccggccg ggatcgagaa gcgcgcgatc
     5701 gccttgagga agccctccgg gcgccacggc ccggtcgacg gggcgagggc gaggaagaag
     5761 gcggggatgc cgatcgtcag cgacgaggtc agggtgaact ggcgcggcag cagcgggaag
     5821 acgccgctgg tcagggcgat cgtgaccagc aggaaggcgg tgaagatcgc cttgctgacg
     5881 aacagccggg cgacgcgctg gatgttgcgg aggatctggc ggccctcgtg aaccatcggc
     5941 gggacctcgc cgaactcgcc ggagacgagg accaggtcgg agaccgagcg cgccatctgg
     6001 gtgcccgagc cctgggcgat cgccagccgc gcctgcttca gggccggcac gtcgttgacg
     6061 ccgtcgccga gcatgccgac gtactcgccg ccctcggcga gcacgcgcac gacccgggcc
     6121 ttgtcctcgg gcgagatccg gccgatcgcc ggcgccgccc gcaccgccgc cagcagttcc
     6181 tcgtcgccct ccggcagggc gccgccgtcg agcgcgtcgt cgcgggcctc gatgccgacg
     6241 tcgcgggcga tcgccccgac cgtcgccggg ttgtcgccgg agagcacccg cagggtcact
     6301 tcctcggcgg cgaagaagga gacggtgtcg gtcgcgttgg cgcgcagctc ctcggccagc
     6361 acgacgatcc ccagcggccg cagcgccgcc ggcagctccg ccgtggggga ggccgcgggc
     6421 agcgggccgt cggcggcggc gagggcgagc acccggcggc cgtcctcggc gttggcggcc
     6481 gctcgctcgc gcagcgccac cgcggtggcg gcgccgtcga gcaggcgctc gggggcgccg
     6541 aagaccagcc gttcgccgcc
//
LOCUS       DASRIG010000413         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2811422, whole genome shotgun sequence.
ACCESSION   DASRIG010000413 DASRIG010000000
VERSION     DASRIG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811422"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFB52_11035"
     CDS             complement(<1..509)
                     /locus_tag="VFB52_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZO06912.1"
                     /translation="MRRPSLAATVLVLLLSLALPACGSADTTGTGGGATSSAQPGDAG
                     ASAKGGVALKSIGRFDQPVYVTGAPGFPQLLFVVEQPGKIIVLKNGKRLGRPFLNITG
                     PVNDGGEQGLLSVAFPPNYAQSKRFYVFYTAASGAITVDEFRLGSATRALPGSRRNVI
                     SIPHAENEN"
     gene            complement(542..1156)
                     /locus_tag="VFB52_11040"
     CDS             complement(542..1156)
                     /locus_tag="VFB52_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06913.1"
                     /translation="MAALLAACGGESSSDSNERAGTYQLEVTEANFPNEQRLGQTTLL
                     RLAVKNAGDETVPALTVTIGVGGEAGEGSSLPFGVHDASAGLAQADRPVWVLAERYPR
                     FAGSSKPAGASTSNKKTFAFGPLKPGASATAIWKLSAVKAGDWKLNYRVGAGLGTQVH
                     AETPSGSAVGGTLVSTITADLPEVEVTDDGEVVDITSSGKRGSE"
     gene            1372..2202
                     /locus_tag="VFB52_11045"
     CDS             1372..2202
                     /locus_tag="VFB52_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06914.1"
                     /translation="MAPLVEERMVGSAVNRALLPAKSVFETVGEMMLLTGRTIVSAIK
                     PPYPYGEEFIGQFLFALQLCWFPLLVSTVAFGFGAPGLQAANFLSLFGALDRLGGFFV
                     LASIREFAPFVTAVVVAGVAGTAITADLGARKIREELDALQVLGVDPIKNLVVPRFLA
                     LMLITGLLDIYALVFGISGGILTEMLYSQPLGGFFATLFSNASITDLWGSVIKCTLFG
                     AIIAVVCSYKGMTASGGAAGVGRAVNEAVVISFMGVFAFNYAFTQTLLATNPELQTIR
                     "
     gene            2199..>2599
                     /locus_tag="VFB52_11050"
     CDS             2199..>2599
                     /locus_tag="VFB52_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO06915.1"
                     /translation="MSEWLAVPRGWLDSFGEIARFGSRVAGLVYSGRVFRFFGEALRQ
                     AGILILGSALIIWGLVFVLGLQCGIEGAYLLRAQGAPAYAGVFSAWCDLREVMPYAFG
                     YMLSAKVGTGIVAELGAMRISDEIDALEVMGV"
BASE COUNT          339 a          914 c          878 g          468 t
ORIGIN      
        1 tggttctcgt tctcggcgtg ggggatcgag atcacgttgc ggcgcgagcc gggcagcgcg
       61 cgggtggcgc tgccaaggcg gaactcgtcg accgtgatcg ccccgctcgc cgccgtgtaa
      121 aagacgtaga agcgtttgct ctgggcgtag ttgggcggga aggcgaccga gagcagcccc
      181 tgctcgccgc cgtcgttgac cggaccggtg atgttgagga acggccgccc gagccgtttg
      241 ccgttcttca gcacgatgat cttgcccggc tgctcgacca caaacagcag ctgggggaag
      301 ccgggggcgc cggtcacgta gacgggctgg tcgaaccggc cgatcgactt cagggcgacg
      361 ccgcctttgg cgctggcgcc ggcgtcgccg ggctgcgcgc tgctcgtcgc gccgccgccc
      421 gtgcccgtcg tatccgccga cccgcaggcg ggcagcgcca gcgagagcag gagcacgagc
      481 actgtcgcag cgagggaggg gcggcgcatc gtcgccaaac gctacccggc gcggctggtc
      541 tctattcgct gccgcgcttg ccgctggacg tgatgtcgac gacttcgccg tcgtcggtga
      601 cttcgacttc gggcaggtcc gcggtgatcg tggagacgag cgtgccgccg accgcgctgc
      661 cgctcggggt ctcggcgtgt acctgcgtcc ccagcccggc gccgacccgg tagttcagtt
      721 tccagtcgcc ggctttgacc gcgctcagct tccagatcgc cgtcgcgctc gcgcccggct
      781 tcaggggacc gaaggcgaag gtttttttgt tcgaggtcga ggcgccggcc ggtttcgagg
      841 agccggcaaa gcgcgggtag cgttcggcca gcacccagac gggccggtcg gcctgggcga
      901 gcccggcgga cgcgtcgtgt accccgaagg gcagcgatga gccttcgccc gcctcgccgc
      961 cgaccccgat cgtcacggtc agcgccggca ccgtctcatc gccggcgttc ttgaccgcga
     1021 gccgcagcag cgtggtctgg ccgagccgct gctcgttcgg gaagttcgcc tcggtcactt
     1081 ccagctggta cgtgccggcg cgctcgttcg agtccgagga cgattcgccc ccgcaggcgg
     1141 caagcagcgc cgccaccgcc acagcgatca acccagtcgc cgcggtccgg aggcgggtgt
     1201 gccgggaatg tcttccggtc ccccgcaaag ccggtcgaag ataccacggg gtttcccagc
     1261 atccgggcgg ttttcgaagc ggcggatcgg gttagtcgta tctcaatacg cggctaacgg
     1321 aaggggctga tccatcccat agactcctcg cagagtgggg cgtcgcgggg tgtggcgcca
     1381 ctcgtggagg agcgaatggt cggatccgcg gtcaacaggg cgcttctgcc cgccaagtct
     1441 gtgttcgaaa cggtcgggga gatgatgctc ctgaccgggc gtaccatcgt ttccgcgatc
     1501 aaaccgccgt acccctacgg cgaggagttc atcggccagt tcctctttgc cctgcagctc
     1561 tgctggttcc cgctgctggt ctcgaccgtg gccttcggct tcggcgcccc gggcctgcag
     1621 gcggccaact tcctctcgct gttcggcgcc ctcgaccggc tcggcggctt cttcgtgctc
     1681 gcctcgatcc gcgagttcgc gcccttcgtg accgcggtcg tcgtcgccgg cgtcgccggc
     1741 accgcgatca ccgccgatct gggagcccgc aagatccgcg aggagctcga cgccctgcag
     1801 gtgctcggcg tcgacccgat caagaacctg gtcgtgccgc gcttcctggc gctgatgctg
     1861 atcaccggcc tgctcgacat ctacgccctg gtcttcggga tcagcggcgg catcctcacc
     1921 gagatgctct acagccagcc gctcggcggc ttcttcgcca cgctcttctc caacgcctcg
     1981 atcaccgacc tctggggctc ggtgatcaag tgcaccctgt tcggcgcgat catcgccgtc
     2041 gtctgctcat acaaagggat gactgcgtcc ggcggcgccg ccggcgtcgg acgcgccgtc
     2101 aacgaggccg tggtgatctc gttcatgggc gtcttcgcct tcaactacgc cttcacccag
     2161 accctgctcg ccaccaaccc ggagctgcag acgatccgat gagcgaatgg ctcgcagtcc
     2221 cgcgcggctg gctcgactcc ttcggggaga tcgcccgctt cggctcccgc gtcgccggcc
     2281 tcgtctactc cggccgcgtc ttccgcttct tcggcgaggc cctgcgccag gccgggatcc
     2341 tgatcctcgg ctcggccctg atcatctggg gcctcgtctt cgtgctcggc ctgcagtgcg
     2401 ggatcgaggg cgcctacctg ctgcgcgccc agggcgcacc cgcctacgcc ggcgtcttct
     2461 ctgcctggtg cgacctgcgc gaggtgatgc cctacgcctt cggctacatg ctctcggcga
     2521 aggtcgggac cgggatcgtc gccgagctcg gcgctatgcg catctcggac gagatcgacg
     2581 cgctcgaggt gatgggcgt
//
LOCUS       DASRIG010000414         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_725745, whole genome shotgun sequence.
ACCESSION   DASRIG010000414 DASRIG010000000
VERSION     DASRIG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725745"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="VFB52_11055"
     CDS             complement(<1..1405)
                     /locus_tag="VFB52_11055"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZO06916.1"
                     /translation="MSDLTTAPALTTHAKLLQWVGEIAALTQPDSIYWCDGSAEEYDR
                     LAQVLIDAGTFEKLSDAKRPNSYLALSDPADVARVEDRTFICSEREEDAGPTNHWKAP
                     AEMREELNGFFKGCMVGRTMYVVPFSMGPVGSEISEIGVQLTDSAYVATNMRIMTRMG
                     KAALDVLGEDGSFVPCVHSVGMPLTEGVEDVPWPCNGDNKYIVHYPETREIWSFGSGY
                     GGNALLGKKCFALRIASAMARDEGWLAEHMLILKLTSPEGTSKFIGGAFPSACGKTNL
                     AMLIPTLPGWKVETVGDDIAWMKFGPDGRLHAINPEAGFFGVAPGTGEKTNPNAMATI
                     RKCSIFTNCARTDDGDVWWEGMTKEPPAHAIDWHGEDWTPDSDHPAAHPNARFTTPAS
                     QCPSIADEWESPAGVPIDAILFGGRRATNVPLVREALDWEHGVFMGSIMSSETTAAAS
                     GDVGKLRYDPFAMLPFCG"
     gene            complement(1415..1690)
                     /locus_tag="VFB52_11060"
     CDS             complement(1415..1690)
                     /locus_tag="VFB52_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HZO06917.1"
                     /translation="MALLIALPFAAAGCGDTVIDSSKTEDTIQASLEKSLGGEIKAVD
                     CPTDQKVEADTTFDCTVVFPGGDKETVTLKIRNSDADVSIIGLEKQK"
     gene            complement(1723..3681)
                     /locus_tag="VFB52_11065"
     CDS             complement(1723..3681)
                     /locus_tag="VFB52_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZO06918.1"
                     /translation="MEATSMRPQPRNLDFARRIEWDGVITWLLCFGLVAYLGIEGGGF
                     DALVYDQVGIGIWWIVAVGALVAALPRSGPSHLALAAWVLLAAFAAWTALSMFWTESG
                     ERTFADIARMLTYLGAFTLTLAVRAPREPRRLVAALAAAIALIGLIGLLSRLHPAWFP
                     ASAEQTARFLNDSRERLSYPLNYWNALGGLIAIGVPLLLVVAGSAKTIVVRGLAAAAV
                     PPLLLALFYTLSRGGIGAAALAVVVFIAFSDNRVPKIFTALITGAGGAILIAIAAGKE
                     QLRHGLSGPVAHDQGDEMLLIAVLVCAAVAVVAVLVGKFFESERRPAWTEPSREFSLV
                     AVGAAVLAVIVIALAANAPDRISNAVEEFKHGGNAGTGAGRLNSFAGESRYELWKSAI
                     DQNSTKPLTGTGSGTFVYWWDRDAAGDEPVQDAHSLYLQVLGELGIVGFLLLGGFVAL
                     VLLSGGLLAVRAGPELRPWLAAGLGGAIAFFSAAAIDWSWQMPVLPIAALMLSAMLVM
                     APAAVPGAESVLRRWPVRIGLALGALIAVVAIAVPLASTSLIRSSEAAVRDGELDKAL
                     EDARSAQNVEPGAATPRLQQALVLELQGNLPAAAEAATEATERESTNWRTWLVLSRVE
                     ALQGNADEALAAYRKAKSLNPLSPLFDR"
     gene            3749..4366
                     /locus_tag="VFB52_11070"
     CDS             3749..4366
                     /locus_tag="VFB52_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06919.1"
                     /translation="MKKPFTLLAIVAALALVMALPSAAAAEYLIPDGNSAVNQYTEGI
                     PTGGGEKDTEGSGQDDVKPGKTLGAKNAHKLESKGEEGKAAAELAAETAPPTVVPEEE
                     STSGDGGKQDDGKQNGKGNKGDGKDSEPDDNGSEEANTGGGVVSNDPGPGGSSGLGEL
                     VSAATGGSDGGIGLLLPLTIFAALVWGIVFAVRRRDHSPEPSARP"
BASE COUNT          714 a         1496 c         1525 g          638 t
ORIGIN      
        1 atccgcagaa cggcagcatc gcgaacgggt cgtagcgcag cttgcccacg tcgcccgacg
       61 cggcggccgt ggtctcggac gacatgatcg aacccatgaa cacgccgtgc tcccagtcga
      121 gcgcctcgcg cacgaggggc acgttcgtcg cccgccgtcc gccgaacagg atcgcgtcga
      181 tcggcacgcc ggcggggctc tcccactcgt ccgcgatcga agggcactgc gaggccgggg
      241 tcgtgaagcg tgcgttcggg tgagcggcgg gatggtcgga gtcgggcgtc cagtcctcgc
      301 cgtgccagtc gatcgcgtgc gcgggcggct ccttggtcat gccctcccac cacacgtcgc
      361 cgtcatcggt ccgcgcgcag ttcgtgaaga tcgagcactt gcggatcgtt gccatggcat
      421 tcgggttcgt cttttccccg gtgccgggcg cgacgccgaa gaacccggcc tcgggattga
      481 tcgcatgcag gcggccgtcg ggaccgaact tcatccacgc gatgtcatcg ccgaccgtct
      541 cgaccttcca gccgggcagg gtcgggatca gcatcgccag gttggtcttg ccgcaggccg
      601 aggggaaggc gccgccgatg aacttcgagg tgccctccgg cgaggtcagt ttgaggatca
      661 gcatgtgctc ggccagccaa ccctcgtcgc gggccatcgc cgaggcgatc cgcagcgcga
      721 agcacttctt gccgagcagg gcgttgccgc cgtagcccga gccgaaggac cagatctcgc
      781 gcgtctccgg gtagtggacg atgtacttgt tgtcgccgtt gcagggccac ggcacgtcct
      841 cgacgccctc ggtcagcggc atcccgaccg agtgcacgca ggggacgaag gagccgtcct
      901 cgccaagcac gtcgagggcg gccttgccca tccgggtcat gatccgcatg ttggtggcga
      961 cgtaggcgct gtcggtgagc tgcacgccga tctcggagat ctcggagccg accgggccca
     1021 tcgagaacgg gaccacgtac atcgtgcgcc cgaccatgca gcccttgaag aagccgttca
     1081 gctcctcacg catctcggcg ggggctttcc agtggttggt cggccccgcg tcctcctcgc
     1141 gctcggagca gatgaaggtg cggtcctcga cccgggcgac gtccgccgga tcggagaggg
     1201 cgaggtagga gttcggccgc ttcgcgtcgg agagcttctc gaaggtgccc gcgtcgatca
     1261 ggacctgggc gagccggtcg tactcctcag cggatccatc gcaccaatag atggaatccg
     1321 gctgggttag ggcggcgatc tcaccgaccc actgcagcag tttggcgtgt gtggtcaacg
     1381 cgggggcggt ggtcaggtcg ctcattcgcc ttccttattt ctgtttctcg aggccgatga
     1441 tcgagacgtc ggcatcgctg tttctgatct tcaaggtcac ggtttccttg tcgccgccag
     1501 ggaagacgac ggtgcagtcg aaggtggtgt cagcttcgac cttctggtcg gtgggacagt
     1561 cgacggcttt gatctcgccg ccaagtgatt tttcaagact cgcctggatg gtgtcctcgg
     1621 tcttgctgga atcgatcacc gtgtcgccac agccagcggc ggcgaacggt agcgcaatca
     1681 gaagcgccaa agctaagcaa tatcgggatt ttggccaggg gctcaacgat cgaacagggg
     1741 cgaaagcggg ttcagtgact tcgccttacg gtatgcggcg agcgcctcgt ccgcgttccc
     1801 ctgcagggct tctacgcgcg agaggaccag ccaggtgcgc cagttggtcg actcgcgctc
     1861 ggtcgcctcg gtcgccgctt ccgccgccgc cggcaggttc ccctgcagct cgagcacgag
     1921 cgcctgctgc aggcgcgggg tcgccgcgcc gggctcgacg ttctgggcgc tgcgcgcgtc
     1981 ttccagcgcc ttgtcgagct caccgtcgcg gaccgcggcc tcgctgctgc ggatcaggct
     2041 ggtcgaggcg agcggcacgg cgatcgcgac gaccgcgatc agggcgccga gcgccagccc
     2101 gatccggacc ggccagcgcc gcagcaccga ctccgcgccg ggcacagccg ccggcgccat
     2161 caccagcatc gcgctcagca tcagtgccgc gatcggcaag accggcatct gccagctcca
     2221 gtcgatcgcg gcggcggaga agaaggcgat cgcgccgccc agcccggcgg ccagccacgg
     2281 ccgcagctcc ggccccgccc gcaccgccag gagcccaccg ctgagcagga cgagggcgac
     2341 gaagccgccc aacagcagga agccgacgat cccgagctcg cccagcacct gcaggtagag
     2401 cgagtgcgcg tcctggaccg gctcgtcgcc ggccgcgtcg cggtcccacc agtagacgaa
     2461 ggtgcccgag ccggtgccgg tcaggggctt ggtcgagttc tggtcgatcg ccgacttcca
     2521 gagctcgtag cggctctcgc cggcgaagct gttcagccgt ccggccccgg ttccggcgtt
     2581 gcccccgtgc ttgaattcct cgaccgcgtt ggagatccgg tcgggcgcgt tggcggctag
     2641 ggcgatcacg atcaccgcga gcaccgccgc gccgaccgcg accagcgaga actcgcggct
     2701 cggttcggtc caggcgggtc gccgctcgct ctcgaagaac ttgccgacga ggaccgcgac
     2761 gacggcaacg gcggcgcaga cgagcaccgc gatcagcagc atttcgtcgc cctgatcgtg
     2821 ggccaccggg ccggagaggc cgtggcggag ctgctctttg ccggcggcga tcgcgatcag
     2881 gatcgcaccg ccggcaccgg tgatcagggc ggtgaagatc ttcggcacgc ggttgtcgct
     2941 gaaggcgatg aagacgacga cggcgagcgc cgcggcgccg atcccgccgc gcgagagcgt
     3001 gtagaagagc gccagcagca gcggcggcac cgcggcggcg gcgagcccgc gcacgacgat
     3061 cgtcttcgcg ctgccggcca cgaccagcag cagcggcacg ccgatcgcga tcaggccgcc
     3121 gagcgcgttc cagtagttga gcgggtagga gagccgttcg cggctgtcgt tgaggaagcg
     3181 cgcagtctgc tcggcgctgg ccgggaacca ggccggatgc aggcgcgaga gcagcccgat
     3241 cagcccgatc aaggcgatcg cggcggccaa cgcggcaacc aggcggcgcg gttcgcgcgg
     3301 cgcccgcacc gccagggtga gggtgaaggc gccgagatag gtgagcatgc gggcgatgtc
     3361 ggcaaaggtg cgctcgccgc tctcggtcca gaacatgctg agcgcggtcc aggcggcgaa
     3421 ggcggcgagc agcacccagg ccgccagcgc cagatgactc ggcccgctgc gcggcagcgc
     3481 cgcgaccagg gcgccgaccg cgacgatcca ccagatcccg atcccgacct ggtcgtagac
     3541 gagggcgtcg aagccgccgc cttcgatccc gagataggcg acgagcccga agcagagcag
     3601 ccaggtgatc accccgtccc actcgatgcg ccgggcgaaa tccaggtttc gcggctgcgg
     3661 ccgcatcgaa gtcgcctcca tccggttcta tgctacgagc gcgggtcgga cgccggatag
     3721 tggccggtgc agcccgcgag gccacctaat gaagaagccg ttcacactcc tggcgatcgt
     3781 cgcggcactg gctctggtca tggccttgcc atcggctgcc gctgccgaat acctgatccc
     3841 cgacggcaac tctgccgtca accagtacac cgagggcatc cccaccggcg gtggcgaaaa
     3901 ggacaccgag gggtccggcc aggacgacgt caagccgggc aagacgctcg gggcgaagaa
     3961 cgcgcacaag cttgaaagca agggcgagga aggcaaggcc gcggccgagc tggccgccga
     4021 aaccgccccg ccgaccgtcg tgccggagga agaaagcacc agcggcgatg gcggtaagca
     4081 ggacgacggc aagcagaacg gcaagggcaa caagggcgac ggcaaagact ccgagcccga
     4141 cgacaacggc tccgaagaag cgaataccgg cggcggcgtg gtctcgaacg accccggccc
     4201 cggtggctcc tccggcctcg gtgagctcgt ctccgccgcg accggcggct ccgacggcgg
     4261 catcggcctg ctgctgccgc tgacgatctt cgccgccctg gtctggggca tcgtcttcgc
     4321 ggtgcgccgc cgcgatcact ctccggagcc ctcagcgcgc ccgtgacccg cac
//
LOCUS       DASRIG010000415         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_725763, whole genome shotgun sequence.
ACCESSION   DASRIG010000415 DASRIG010000000
VERSION     DASRIG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725763"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFB52_11075"
     CDS             complement(<1..254)
                     /locus_tag="VFB52_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HZO06920.1"
                     /translation="MKAKDKQAYLEEYELLKKKGKPFFPYAVMKDSTMALIVVAVIIV
                     MSLILGAEQGPKADPTTTTYVPRPEWYFFFLFEVLRVIKPP"
     gene            complement(251..1213)
                     /locus_tag="VFB52_11080"
     CDS             complement(251..1213)
                     /locus_tag="VFB52_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZO06921.1"
                     /translation="MPKLPSLKIPDAVVPPPLRKPPKPGAAGAPKGDAPSDLKSSPPP
                     SKTHGDRVLDQAKDAPAEVLAWVDQRTGATGFLTGMLYRKVPKGTNWFYTLGSATLFA
                     FIVQAVTGVFLAMFYTPSATQAYASISHINNEVPLGALVHGMHKWGSSVMVILIFLHM
                     GRTFFFGAYKYPRELNWVIGVVLLILTMTMAFTGYLLPFDQRSFWASVVGININGTGP
                     IVGPYLSDFLRAGPELGATTLSRFYAIHMLLVPGAIIALIGAHLYLVVKLGTTAPPWV
                     KAERPKIASPVAELEAGAGNGHVNGHVNGYERVDGHDGTGGTSA"
     gene            complement(1213..1791)
                     /locus_tag="VFB52_11085"
     CDS             complement(1213..1791)
                     /locus_tag="VFB52_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZO06922.1"
                     /translation="MTRRRAFTIAAQGLGGLAGAAIILPAVGFAVAPIFHRGKERWEG
                     VGPTSDFVEDTYRQVVFTETEGIGDAGKTTAYVRKGSKLYDEDPNSFVAVSNRCAHLG
                     CPVRFVEAAGNFICPCHGGVYDFEGKRIGGPPVRPLDQFQTRVRNGQVEVGPRYSVTN
                     QMEPVRARDPGEFTGGIWEYLYPPRPSTFPAP"
     gene            complement(1918..2370)
                     /locus_tag="VFB52_11090"
     CDS             complement(1918..2370)
                     /locus_tag="VFB52_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06923.1"
                     /translation="MSGRPEDALRALAAQLREEGTPISPHVVEPAFAPQLGALAARGE
                     RAAAAPGEYAFVIEAVREGYLLHYGEPRVLAGHDDHLALLAGDYLYALGLDRLAQLGD
                     TAAVAVLADLISRCAQLHAEGRAEAVPQLWQDAARAIGGGNPPLRPTQ"
     gene            complement(2367..3713)
                     /gene="hemL"
                     /locus_tag="VFB52_11095"
     CDS             complement(2367..3713)
                     /gene="hemL"
                     /locus_tag="VFB52_11095"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZO06924.1"
                     /translation="MPRPSTRRSACTTSPTTTRNGRRLIPEPGPGGLYERALRVLPGG
                     VNSPVRAMRQIGRDPIFIERGEGCEITSVEGVRYLDWVGSWGPLILGHAEPAVVAAVI
                     EAAKRGTSYGAATAYEVELAEQIVDRFPAVEMVRMTSSGTEAAMGAVRLARAITGREV
                     VVKFAGAYHGHSDGLLADAGSGLATLGVPGSPGVTAAQAAGTIVVPWNDREAVAKALA
                     EHEVAALLAEPIAANMGVVPPAEGFLEFLREQTTASGALLVFDEVITGFRVARGGAQE
                     LYGVDPDLTVMGKVIGGGLPAAAFAGPRAAMERIAPAGDVYQAGTLSGNPLAVAAGLA
                     TLAQLDADAYARLGDLTDRLAAGFAELAADRPLQVASAPGLVTLFFSSEPVTDFAGAS
                     ACDTAAHARFCNAMLDRGVYPPPSQFEAWFVSLAHTESTVDRTLEAAGEALTEALA"
     gene            complement(3634..4719)
                     /locus_tag="VFB52_11100"
     CDS             complement(3634..4719)
                     /locus_tag="VFB52_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZO06925.1"
                     /translation="MASVKVPQSKARSRKDGAAIPLDETDKRLMNLLQSSFPLDPEPF
                     ALVASEAELELDDVLARTQRLLENRIIREITPIFDTRALGYESMLVAAKVDSENPQRA
                     AKVVNAHPGVSHNYLRTHEFNLWFTIATPPDSELGLSGTLQALMEEAGAESMRELPTL
                     TLFKINMNLEMEKGTEALASTVEAAPPRELEMQPYDEDDIATIKALQGPMAAVERPYD
                     EAAAELGISTDDLLERLRTMVDRKILRRVAAILYHRRAGFSANGMGVWKVPEDQILEV
                     GGRMAAFRGISHCYQRPTYEDWPYSVFTMAHGRSKEECDAILDSIAQECGMGPEDRAT
                     LYSSTEYKKIRLHYFTNDYAEWEAAHS"
     gene            complement(4707..>5512)
                     /gene="hemB"
                     /locus_tag="VFB52_11105"
     CDS             complement(4707..>5512)
                     /gene="hemB"
                     /locus_tag="VFB52_11105"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZO06926.1"
                     /translation="NNLTEEAGEIAAAGIGAVILFGIPGAKDEVGTGAYDEEGIVQMA
                     VRALKEAHPDLTVITDVCLCEYTSHGHCGVFREGGTEVDNDLSVELLAKIAISHADAG
                     ADAVAPSDMMDGRIGSIRFQLDEEGHPDVPIIAYSAKYASAFYGPFRDAAESTPEFGD
                     RRGYQMDPANAAEAVREAELDLDEGADMLMVKPATPYLDVVRRVKDATGAPLAAYHVS
                     GEYSMLKAATANGWLDERAAVLETLTSIRRAGADAIVTYYAKEAAPWLQ"
BASE COUNT          786 a         1834 c         2030 g          862 t
ORIGIN      
        1 ggcggtttga tcacgcgcag gacctcgaag aggaagaaga agtaccactc ggggcgcggc
       61 acgtaggtgg tcgtcgtcgg gtcggccttc ggtccctgct cggcgccgag gatcagcgac
      121 atgacgatga ttacggcgac gacgatcagc gccatcgtcg agtccttcat caccgcgtag
      181 gggaagaacg gcttcccctt cttcttcagc agttcgtact cttcgaggta ggcctgcttg
      241 tctttggcct tcatgccgac gtgccccccg tcccgtcgtg gccgtcgacc cgctcgtagc
      301 cattcacgtg cccgttgacg tgcccgttcc cggcgccggc ctcgagctcg gcgaccggcg
      361 acgcgatctt cggccgctcg gctttgaccc acggcggggc ggtcgtgccg agcttcacca
      421 ccaggtagag gtgggcgccg atcagggcga tgatcgcccc ggggacgagc agcatgtgga
      481 tcgcgtagaa gcgcgagagc gtcgtcgccc cgagctcggg gccggcgcgc aggaagtcac
      541 tcaggtaggg gccgacgatc gggcccgtgc cgttgatgtt gatgccgacg accgaggccc
      601 agaaggagcg ctggtcgaac ggcagcaggt agccggtgaa ggccatcgtc atcgtcagga
      661 tcagcaggac cacgccgatc acccagttga gctcgcgcgg gtacttgtag gcgccgaaga
      721 agaaggtgcg ccccatgtgc aggaagatca ggatcaccat cacgctcgag ccccacttgt
      781 gcatcccgtg gaccagggcg ccgagcggta cctcgttgtt gatgtgcgag atcgaggcgt
      841 aggcctgggt cgccgagggc gtgtagaaca tcgccaggaa gacgccggtt acggcctgga
      901 cgatgaaggc gaagagggtc gcggagccga gcgtgtagaa ccagttggtc cctttcggca
      961 ccttccggta gagcatcccg gtgaggaagc cggtcgcacc ggtgcgctgg tcgacccagg
     1021 cgaggacctc ggcgggcgcg tccttggcct ggtcgagaac ccggtcgccg tgcgtcttgc
     1081 tcggcggcgg tgagctcttc aggtccgagg gagcgtcgcc cttgggcgcg ccggcggcgc
     1141 cgggcttcgg cggtttgcgc agcggcggcg gtacgaccgc gtccgggatt ttcagcgacg
     1201 gcagtttcgg catcagggtg ctgggaaggt agagggacgc ggcgggtaga ggtattccca
     1261 gatcccgccg gtgaactccc cggggtcgcg ggcgcggacc ggctccatct ggttggtcac
     1321 gctgtagcgc ggaccgacct caacctggcc gttgcgaacc cgggtctgga actggtcgag
     1381 cgggcggacc ggcggaccgc cgatccgctt gccctcgaag tcgtagacgc cgccgtggca
     1441 ggggcagatg aagttgcccg cggcctcgac gaagcggacc gggcagccga ggtgggcgca
     1501 gcggttggag accgcgacga agctgttcgg gtcctcgtcg tagagcttcg agcctttgcg
     1561 cacgtaggcg gtggtcttgc cggcgtcgcc gatcccctcg gtctcggtga agacgacctg
     1621 gcggtaggtg tcctcgacga agtcgctggt cggcccaacc ccctcccagc gctccttgcc
     1681 gcggtggaag atcggggcga cggcgaagcc gaccgcaggc aggatgatcg ccgcgccggc
     1741 gaggccgccg aggccctggg ccgcgatcgt gaaagcgcga cgccgagtca tcgactcgcc
     1801 ctcgaagtag ccaggtgggt cgggctttac ccgctcctgc tgaggggccc cattggcccc
     1861 gttcttcttg tccgcgctat ctgctgccac agtcgatccg ggattcggga gcggagtcta
     1921 ttgcgttggc cgaagagggg gattaccgcc tccgatagcc cgggcggcgt cttgccagag
     1981 ctgcggcacg gcctcggcgc gaccctcggc gtggagctgc gcgcagcggc tgatcaggtc
     2041 ggctaggacc gccactgcgg cggtgtcgcc gagctgcgcc aggcggtcca ggccaagcgc
     2101 ataaaggtaa tcgccggcca gcaaagcgag gtggtcgtcg tggccggcga gcacccgcgg
     2161 ctcaccgtaa tggagcaggt agccctcacg caccgcctcg atcacaaacg cgtactcgcc
     2221 cggcgccgcg gctgcgcgct cgccccgggc ggccagcgcg ccgagctgcg gggcgaaggc
     2281 cggttcgacc acgtgcgggg agatcggcgt gccctcctcg cgcagctgcg cggccagcgc
     2341 ccgcagggcg tcctcggggc ggccgctcac gcgagcgcct ccgtgagtgc ctcgcccgcg
     2401 gcttcgagcg tgcggtcgac ggtcgactcg gtgtgggcga gggagacgaa ccaggcctcg
     2461 aactgcgagg gcggtggata gacgccgcgg tcgagcatcg cgttgcagaa gcgggcgtgg
     2521 gcggcagtgt cgcaggcact cgcgccggcg aagtcggtga ccggctcgga gctgaagaag
     2581 agcgtgacca ggccgggggc cgaggcgacc tgcagggggc ggtcggcggc cagctcggcg
     2641 aagccggcgg ccagccggtc ggtgaggtcg ccgagccgcg cgtaggcatc ggcgtcgagc
     2701 tgggccaggg tcgccagccc ggccgcgacc gccagcgggt tcccggagag cgtgcccgcc
     2761 tggtagacgt cgccggccgg ggcgatccgc tccatcgccg cgcgcggccc ggcgaaggcg
     2821 gcggccggca gcccgccgcc gatcaccttg cccatcacgg tcaggtcggg gtcgacccca
     2881 tagagctcct gggcgccccc gcgggcgacg cggaaaccgg tgatcacctc gtcgaagacg
     2941 agcagcgcgc cgctggcggt ggtctgctcg cggaggaact cgaggaagcc ctcggcgggg
     3001 gggacgacgc ccatgttggc ggcaatcggc tcggccagga gcgccgccac ctcgtgctcg
     3061 gccagcgcct tggcaaccgc ctcgcggtcg ttccagggca ccacgatcgt gcccgccgcc
     3121 tgcgccgccg tcaccccggg gctgccgggg accccgagcg tcgccaggcc ggagccggca
     3181 tcggcgagca gcccgtcgga gtggccgtgg taggcgccgg cgaacttgac cacgacctcg
     3241 cggccggtga tcgcccgcgc caggcgcacc gcacccatcg ccgcctcggt cccggagctg
     3301 gtcatccgca ccatctcgac cgcggggaag cgatcgacga tctgctcggc cagctcgacc
     3361 tcgtaggcgg tcgcggcgcc gtagctggtc ccgcgcttgg ccgcttcgat caccgccgcg
     3421 accacggcgg gctcggcgtg gccgaggatc agcgggcccc aggagccgac ccagtcgagg
     3481 taccggacgc cctcgacact cgtgatctcg cagccttcac cgcgttcgat gaagatcggg
     3541 tcgcggccga tctggcgcat cgcccgcacc ggcgagttga ctccgccggg gaggacccgt
     3601 agcgcgcgct cgtagagccc gccgggtccc ggctcaggaa tgagccgcct cccattccgc
     3661 gtagtcgttg gtgaagtagt gcaggcggat cttcttgtac tcggtcgagg aatagagggt
     3721 ggcgcggtcc tcggggccca tcccgcactc ctgcgcgatc gagtcgagga tcgcgtcgca
     3781 ctcttctttc gagcgaccgt gggccatggt gaagacgctg taaggccagt cctcataggt
     3841 ggggcgctgg tagcagtggg agatcccgcg gaaggcggcc atccgcccgc cgacctcgag
     3901 gatctggtcc tcgggcacct tccagacgcc catcccgttg gccgagaagc cggcccgccg
     3961 gtggtagagg atcgccgcga cccggcgcag gatcttgcgg tcgaccatgg tgcgcaggcg
     4021 ctcgagcagg tcgtcggtgg agatgccgag ctcggcggcg gcctcgtcgt agggccgctc
     4081 gaccgcggcc atcgggccct gcagcgcctt gatcgtcgcg atgtcgtcct cgtcgtaggg
     4141 ctgcatttcg agctcacgcg ggggtgcggc ttcgaccgtc gaggcgagcg cctcggtgcc
     4201 cttctccatc tccaggttca tgttgatctt gaacagggtg agggtcggca gctcgcgcat
     4261 cgactcggcc ccggcctcct ccatcagcgc ctgcagcgtg ccgctgaggc caagctcgga
     4321 gtcgggcggg gtggcgatcg tgaaccagag gttgaactcg tgcgtgcgca ggtagttgtg
     4381 ggagacgccg gggtgggcgt tgacaacctt cgccgcccgc tgcgggttct cggagtcgac
     4441 cttggccgcg accagcatcg actcgtagcc gagcgcgcgg gtgtcgaaga tcggcgtgat
     4501 ctcgcggatg atccggttct cgagcaggcg ctgggtgcgg gcaagcacgt cgtcgagctc
     4561 gagttcggcc tccgaggcga ccagggcaaa cggctcgggg tcgagcggga agctcgactg
     4621 cagaaggttc atcagccgct tgtcggtctc gtcgagcggg atcgctgcgc cgtccttacg
     4681 gctgcgagcc ttcgactgcg ggacctttac tgaagccacg gagcggcctc cttcgcgtag
     4741 taggtgacga tcgcgtcggc gccggcgcgg cggatcgagg tcagggtctc gagcacggcg
     4801 gcgcgctcgt cgagccagcc gttggcggtg gcggccttca gcatcgagta ctcgccggag
     4861 acatggtagg cggcgagcgg cgccccggtc gcgtccttga cccgccgcac gacgtcgagg
     4921 tagggggtcg ccggcttgac catcagcatg tcggcgccct cgtcgaggtc gagctcggcc
     4981 tcgcgcaccg cttcggcggc gttggccggg tccatctggt acccgcgccg atcgccgaac
     5041 tccggggtcg actcggcggc gtcgcggaag gggccgtaga aggcggaggc gtacttggcg
     5101 ctgtaggcga tgatcggcac gtcggggtgc ccctcctcgt cgagctggaa gcgaatcgag
     5161 ccgatccggc cgtccatcat gtcgctcggc gcgaccgcgt cggcgcccgc gtcggcgtgg
     5221 gagatcgcga ttttcgccag cagctcgacc gagagatcgt tgtcgacttc ggtgccgcct
     5281 tcgcggaaga cgccgcagtg accgtgcgag gtgtactcgc agaggcagac gtcggtgatc
     5341 acggtcaggt cggggtgcgc ctccttcagc gcccgcaccg ccatctgcac gatcccctcc
     5401 tcgtcgtagg cgcccgtgcc gacctcgtcc ttggcgccgg ggattccgaa gaggatcacc
     5461 gcgccgatcc cggccgcggc gatctccccc gcctcctcgg tcaggttgtt ga
//
LOCUS       DASRIG010000416         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1509042, whole genome shotgun sequence.
ACCESSION   DASRIG010000416 DASRIG010000000
VERSION     DASRIG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509042"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1517)
                     /locus_tag="VFB52_11110"
     CDS             complement(18..1517)
                     /locus_tag="VFB52_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZO06927.1"
                     /translation="MKTSRSERLLLTALVFVVGASSLGAEIAAARLLAPYFGASTIVW
                     ANTIGVVLVALSIGYWFGGRYADRHPHLRPLCLLVLGAAALIAVIPFAARPFLDFSVE
                     AFDSVSVGTFAGSLFGVLVLVAVPVTMLGAASPWAVRIAVGEPDSTGEVVGRLYAIST
                     FGSLLGTMTSALLFIPLLGTQRTFIAFAFCLALVATIGIGWRAAAVPALLAVLLTVPV
                     GTIKGSGEGEVLYEDETTHQYARVIERDDGSRVLELNEGQAIHSLYRPGEYLTDGYWD
                     GHLVLSFAAQDEPPARIAILGNAGGTVARAFGRYFPETQVDAVEIDGELTELGERFLG
                     LHNSRMTVYEEDARPWLRDAEGGYDAIMVDAYRQPYIPFYLTTEEFFKLCHDRLAPGG
                     VVIVNVGHPESSDELERVLGRTMEAAFPRVMRYPIEDRNTLLVGGDADLSAARLRAAA
                     DTLPDDLEPLALDAAAALEPRLEGGEVYTDDHAPVEWLIDESLLEYANE"
     gene            complement(1498..2412)
                     /locus_tag="VFB52_11115"
     CDS             complement(1498..2412)
                     /locus_tag="VFB52_11115"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZO06928.1"
                     /translation="MTPPYALAGALLGLLELLPLLAAAGLYAKRSMTLAAKGRPVPLW
                     RQICFAAGLLVIFAAIASPIDYIAEELVIAHMVEHLLIGDVATLLLVLGLTGPLLQPL
                     LAIKFFDRLKVLAHPLVAFPLWAANLFFWHIPAMYDAAYGGAFLHAVEHSSFIFFGAL
                     MWMPVFGPLPKPEWFTAAWEVVYVIVVRFTGAILGNILMWSGTVLYDRYTVGELEWGL
                     TPLQDQSTAGVIMMIEGTFLGLGLLAYVFFQVAREGIEKQRLLDLAHDRGIELDPRRA
                     QRAVAAGHTDRLEKQLTGAGRDPENEPV"
     gene            2484..>2748
                     /locus_tag="VFB52_11120"
     CDS             2484..>2748
                     /locus_tag="VFB52_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HZO06929.1"
                     /translation="MSSADHSFPPIGSYGFLSDCHTSALLSYDGAIEWLCLPRFDSAS
                     TFGALLDRGAGHFKLGPRGVIVPLSRRYEPGTLVIETTWVCDSG"
BASE COUNT          455 a          949 c          982 g          362 t
ORIGIN      
        1 caccggtttg caggccgtca ctcattcgcg tattccagca gggactcgtc gatcagccac
       61 tcgaccgggg cgtggtcgtc ggtgtagacc tcgcccccct ccaggcgcgg ctccagcgct
      121 gccgccgcgt ccagggccag cggctccagg tcatccggaa gggtgtcggc ggcggcccgc
      181 agccgggccg ccgagaggtc cgcgtcgccg ccgaccagca gggtgttccg gtcctcgatc
      241 gggtagcgca tgacgcgcgg gaaagcggcc tccatcgtcc gccccagcac ccgctcgagc
      301 tcgtcgctgg actccgggtg gccgacgttg acgatcacca cgccgccggg cgcgagccgg
      361 tcgtggcaga gcttgaagaa ctcctcggtg gtcaggtaga aggggatgta gggctggcgg
      421 taggcatcga ccatgatcgc gtcgtagccg ccctcggcat cgcgcaacca gggccgcgcg
      481 tcctcctcgt agacggtcat ccgcgagttg tggagaccga ggaagcgttc gcccagctcg
      541 gtcagctcgc cgtcgatctc gaccgcgtcg acctgcgtct cggggaagta gcggccgaag
      601 gcgcgggcga cggtgccgcc ggcgttgccg agaatcgcga tccgcgccgg cggctcgtcc
      661 tgcgccgcga acgagagcac caggtgcccg tcccagtagc cgtcggtcag gtactcgccg
      721 ggccggtaga gcgagtggat cgcctggccc tcgttcagct ccagcacccg gctgccgtcg
      781 tcgcgttcga tcacccgcgc gtactggtgc gtggtctcgt cctcataaag gacctcgccc
      841 tcccccgatc ccttgatcgt ccccaccggc accgtcagca acaccgccag cagcgccggg
      901 acggcggcgg cgcgccagcc gatcccgatc gtcgcgacca gcgccaggca gaaggcaaag
      961 gcgatgaagg tgcgctgggt gccgagcagg gggatgaaga gcagcgccga ggtcatcgtc
     1021 ccgagcagcg agccgaaagt cgagatcgcg tacagcctgc cgaccacctc gccggtgctg
     1081 tcgggctcgc cgaccgcgat ccgcaccgcc cagggcgagg cggcgccgag catcgtcacc
     1141 ggcaccgcga ccaggaccag gacgccgaac agggagccgg cgaaggtgcc gaccgagacc
     1201 gagtcgaagg cctcgaccga gaagtcgagg aagggacgag ccgcgaacgg gattaccgcg
     1261 atcagcgccg ccgccccgag cacgagcagg cagagcgggc gcaggtgcgg gtgccggtcg
     1321 gcgtagcggc cgccgaacca gtagccgatc gagagcgcga cgaggacgac cccgatcgtg
     1381 ttcgcccaga cgatcgtcga ggcgccgaag tagggcgcga gcagacgcgc cgcggcgatc
     1441 tcggcgccga ggctggaggc accgacgacg aagacgagcg ccgtcagcag gaggcgctca
     1501 gaccggctcg ttttcagggt ccctccccgc ccccgtcagc tgcttttcga gtcggtcggt
     1561 gtggccggcg gcgacggcgc gctgggcgcg ccgcgggtcg agctcgatcc cgcggtcgtg
     1621 ggcgaggtcg agcaggcgct gcttctcgat cccttcgcgg gcgacctgga agaagacgta
     1681 ggcgagcagc ccgaggccga ggaaggtgcc ctcgatcatc atgatcacgc ccgccgtgct
     1741 ctggtcctgc aggggagtca acccccactc cagctcgccg acggtgtagc ggtcgtagag
     1801 aaccgtgccc gaccacatca ggatgttgcc gaggatcgcg ccggtgaagc gcacgacgat
     1861 cacgtagacg acctcccagg cggcggtgaa ccactccggc ttcggcagcg gcccgaacac
     1921 cggcatccac atcagggcgc cgaagaagat gaagctggag tgctcgaccg cgtgcaggaa
     1981 ggcgcccccg taggcggcgt cgtacatcgc cgggatgtgc cagaagaaca ggttcgccgc
     2041 ccagagcggg aaggcgacca gcgggtgcgc gagcactttc agccggtcga agaacttgat
     2101 cgcgagcagc ggctgcagca gcggcccggt cagccccagc accagcagga gggtggcgac
     2161 gtcgccgatc aaaaggtgct cgaccatgtg ggcgatcacc agttcctcgg cgatgtagtc
     2221 gatcggcgag gcgatcgcgg cgaagatcac cagcagcccg gcggcgaagc agatctggcg
     2281 ccagagcggc accggccggc ccttcgcggc aagggtcatc gagcgcttgg cgtagaggcc
     2341 ggcggcagcg aggagcggaa gcagctccag gaggccgagg agggcgccgg cgagggcgta
     2401 cggcggggtc atcgcaaatc gcagcgtagc gacgggtttg ccccgggagg cacccgccgc
     2461 cgctggtagc ttggcggcgc cagatgagct cggccgacca ctccttcccc cccatcggca
     2521 gctacggctt cctctcggac tgccacacct cggcgcttct ctcctacgac ggcgcgatcg
     2581 agtggctctg cctgccccgc ttcgactcgg ccagcacctt cggggcgctg ctggaccgcg
     2641 gcgccggcca tttcaagctc ggcccgcggg gcgtgatcgt gccgctcagc cggcgctacg
     2701 agccgggcac gctggtaatc gagaccacct gggtctgcga cagcggct
//
LOCUS       DASRIG010000417         5770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2811938, whole genome shotgun sequence.
ACCESSION   DASRIG010000417 DASRIG010000000
VERSION     DASRIG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811938"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFB52_11125"
     CDS             complement(<1..633)
                     /locus_tag="VFB52_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZO06930.1"
                     /translation="MPNRLAQETSPYLLQHRDNPVDWYPWGSEALGRAREQDRPILLS
                     VGYSACHWCHVMERESFEDPETAAYMNEHFVNVKVDREERPDVDAIYMEAVQSMTGHG
                     GWPMTVFLDPEGVPFYGGTYFPPDDSRGMPSFRMVMEAIVDAWERKREEIRDRAPGTR
                     ARLGAVGELEPNADLPGAAELDDAVARLVGDADRAHGGFGGAPKFPPASAL"
     gene            766..2181
                     /locus_tag="VFB52_11130"
     CDS             766..2181
                     /locus_tag="VFB52_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZO06931.1"
                     /translation="MNSSQGREKQELAVERALAKLEPADREVIRGALDRAEKTEAELR
                     HLADHDPLTGLQNRRRFRAELDQHVSFTARYGGQGAVMIIDIDGLKEVNDRLGHQPGD
                     NLIRRIADILRERVRGTDIVARLSGDEFAVLMPQTDTSGALQLGEDLRAEVADGFPAD
                     SEIGSATISVGIKMFGGKPGANAEAVLVAADQAMYRAKGAGRNRIALFNGSDEDEYAL
                     RRVQTTSARIRDALTHDRLKLATQPIRSLASGGVERYELLLRMTGDSGELLPAASFIE
                     AAERSGMVQELDRWVVSQALTILADRERAGQPVSLHVNISGVSLTDISVLEYIERQLD
                     EGEADPGRCTFEITQTARIDDYEAAAGFADRLTEFGCEVAIDDYGAGFGPFSYLKRIP
                     FDVIKIDGAFIRDMPRNDADQLTVKAIVQIARGLGKRTIAEFVQDEDTTQMLREYGVD
                     MAQGFHLGKPVDLVDELGAAA"
     gene            complement(2182..2715)
                     /locus_tag="VFB52_11135"
     CDS             complement(2182..2715)
                     /locus_tag="VFB52_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO06932.1"
                     /translation="MAADEGTETIGVAGTEEVARAYFDAVTKRDIEGMVACWEPGSLD
                     VLHGIAELSVPADLEAWFGALFAAFPDFTFEVLDVTTSGEKSAVRWHATGTFNGSAKF
                     EGLAPNGGSVDVEGCDVLTVRNGKVVRNDAYTNSVEMLRQLGALPPEGSGQEKAMTAL
                     LNAKTKATAWLAQRRSS"
     gene            complement(2724..4163)
                     /locus_tag="VFB52_11140"
     CDS             complement(2724..4163)
                     /locus_tag="VFB52_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO06933.1"
                     /translation="MNLIEDLMERSPASRLGIVAVDAEANRRDWHFGELIACSAGLSG
                     AFAARGVGKGDVVMTMVGNRIEWVLTLLACWRMGAVALPCNTMLRRHDLELRTAEAEP
                     KLCVGEAELMTELPEGIPTMDLEELAYVLDEDQPQETPAAVEPMDPDDPALIVFTSGT
                     TGNPRGVVHGYRYLFGQEVQATHWFGSRPGDLAWCTTATGWSKSARNVFLAPWLTGAA
                     AVIHDGRFDPAERLEFAEVLGVNVLCQAPTEYRMLAKRTALRPLPALRRMVSAGEPLD
                     PETIGAFREAMGLEPADGYGQTETGQVTANLAGEPIRLGSMGKPLPGIETRIADGELQ
                     LRAATSPTFFSGYLDGERFEGEWWQTGDLVREDEDGFLFFEGRGDDIILSAGYRIGPT
                     EVEAALLSHPAVADAAAVAAPDPERGAVVRAIVVLREREASDALVAELQEHCKRETAP
                     YKFPRIVEFAAELPKTSSGKIKRAQLRAG"
     gene            complement(4184..5389)
                     /locus_tag="VFB52_11145"
     CDS             complement(4184..5389)
                     /locus_tag="VFB52_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06934.1"
                     /translation="MINLEIPKKFKPLATQANQVANEVFRPISRKYDEAEHEYPKELD
                     MLASLIDGMNEGSDIGGAGAAGVRREKSDDGENRNGSNLSTVLGIIELCWGDVGLLLS
                     MPRQGLGNSAIASVATEQQLEHYGGKWAAMAITEPEAGSDSAAIRTTAELDGDEYVLN
                     GEKIFVTSGDRADLIVVWASLDRSKGRAAIKSFIVERDNPGLKLDRLEHKLGIRASDT
                     ANFTLQDCRVPKEALLGDPEVKEKGFAGVMETFDNTRPLVAAMAVGVTRAALEETRRR
                     LSEAGVEIDYDKPALSQHAAAARYLEMEADYEASWLLTMHAAWMADNSKPNSLQASMA
                     KAKAGRTCVEVTLGCVELCGSVGYGENELLEKWARDSKILDIFEGTQQIQLLIVARQL
                     LGKSSSELR"
     gene            complement(5415..>5770)
                     /locus_tag="VFB52_11150"
     CDS             complement(5415..>5770)
                     /locus_tag="VFB52_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO06935.1"
                     /translation="GGARAVLDYVIPYCNDRKAFGEPITHRQSVAFLIADLAIELEGM
                     RLLVHRAAALADHGRPISREAGLVRVQCTAKAMKAGTDGVQLLGGHGFVKEHPVERWY
                     RDLRAAGVMEGALLV"
BASE COUNT          833 a         1998 c         2037 g          902 t
ORIGIN      
        1 cagcgccgag gcgggcggga acttgggcgc gccgccgaag ccgccgtggg cgcggtcggc
       61 gtcgccgacg aggcgggcga cggcgtcgtc gagctcggcg gcgccgggca agtccgcgtt
      121 cggctcaagc tcgccgaccg cgccgagccg ggcccgggtg ccgggggcgc ggtcgcggat
      181 ctcctcgcgt ttgcgctccc aggcgtcgac gatcgcttcc atcaccatcc ggaagctcgg
      241 catcccgcgc gagtcgtccg gggggaagta ggtgccgccg tagaagggca cgccctcagg
      301 gtcgaggaag acggtcatcg gccagccgcc gtggccggtc atcgactgca ccgcctccat
      361 gtagatcgcg tcgacgtcgg ggcgctcctc gcggtcgacc ttgacgttga cgaagtgctc
      421 gttcatgtag gcggcggtct ccgggtcctc gaaggactca cgctccatca cgtgacacca
      481 gtggcaggcc gagtagccga ccgagagcag gatcggccgg tcctgctcgc gcgcccggcc
      541 cagcgcctcg ctgccccagg ggtaccagtc gaccgggttg tcgcggtgct ggagcaggta
      601 gggactggtc tcctgggcga gccggttggg catctgatct caccctaccc ggggccgtgc
      661 gaagccaatt attggacacg ggcgtatcaa cgaaattcgt gtcccctggc ggatttgcgg
      721 atgcccttaa cccctttgtg cggtcgagtc actaacttct tcccagtgaa ctcctcgcaa
      781 ggccgtgaaa agcaggaatt ggcagtcgag cgcgcgcttg ccaagctcga gccggccgat
      841 cgcgaggtga tccgtggggc gcttgaccgg gccgagaaga ccgaggccga gctgcgccac
      901 ctggccgacc acgatccgct gaccgggctg cagaaccgcc gccgcttccg cgccgagctc
      961 gaccagcacg tctccttcac cgcccgctac ggcggccagg gcgcggtgat gatcatcgac
     1021 atcgacgggc tcaaggaggt caacgaccgg ctcggccacc agcccggcga caacctgatc
     1081 cgccggatcg ccgacatcct gcgcgagcgg gtccggggga ccgacatcgt cgcccgcctc
     1141 tccggcgacg agttcgcggt tctgatgccg cagaccgaca cctccggcgc cctgcagctc
     1201 ggcgaggacc tgcgggccga ggtcgccgac ggctttccgg ccgactcgga gatcggctcg
     1261 gcgacgatca gcgtcgggat caagatgttc ggcggcaagc cgggcgccaa cgccgaggcg
     1321 gtgctggttg ccgccgacca ggcgatgtac cgggccaagg gcgcgggacg caaccggatc
     1381 gcgctcttca acggctccga cgaagacgaa tacgccctgc gccgcgtgca gacgaccagc
     1441 gcccggatcc gcgacgcgct cacccacgac cgcctgaaac tggcgacgca gccgatccgc
     1501 agcctcgcct ccggcggggt cgagcgctac gagctgctgc tgcggatgac cggggacagc
     1561 ggcgagctgc tgccggcggc ctcgttcatc gaagccgcgg agcgctcggg catggtccag
     1621 gagctcgatc gctgggtggt cagccaggcg ctgacgatcc tcgccgaccg cgagcgcgcc
     1681 ggccagcccg tctccctgca cgtgaacatc tccggcgtct cgctcaccga catctccgtg
     1741 ctcgagtaca tcgaacgcca gctcgacgag ggcgaagccg accccggccg ctgcaccttc
     1801 gagatcaccc agaccgcgcg gatcgacgac tacgaggccg ccgccgggtt cgccgaccgg
     1861 ctgaccgagt tcggctgcga agtcgcgatc gacgactacg gcgccggctt cggacccttc
     1921 agctacctca aacggattcc cttcgacgtg atcaagatcg acggcgcctt catccgcgac
     1981 atgccgcgca acgacgccga ccagctgacc gtgaaggcga tcgtccagat cgcccgcggg
     2041 ctcggcaagc gcacgatcgc cgagttcgtc caggacgagg acacgaccca gatgctgcgc
     2101 gagtacggtg tcgacatggc ccagggcttc cacctcggca aacccgtcga cctggtcgac
     2161 gaactgggcg ccgcggctta actagctgga gcggcgctga gcgagccagg cggttgcctt
     2221 ggtcttcgcg ttgagcagcg ccgtcatcgc cttttcctgg ccggacccct ccgggggcag
     2281 ggcgccgagc tggcgcagca tctcgacgct gttcgtgtag gcgtcgttgc gaacgacctt
     2341 gccgttgcgc acggtgagca cgtcgcagcc ctcgacgtcg acgctcccgc cgttgggcgc
     2401 gaggccttcg aacttggcgg acccgttgaa ggtgccggtg gcatgccagc gcaccgccga
     2461 cttctcaccg ctggtggtca cgtcgaggac ctcgaaggtg aagtcgggga aggcggcgaa
     2521 cagcgcgccg aaccaggcct cgagatcggc gggcacgctc agctcggcga tcccgtggag
     2581 gacgtccagc gagcccggct cccagcaggc gaccatcccc tcgatgtcgc gcttggtcac
     2641 cgcgtcgaag taggcgcggg cgacctcctc ggtgccggcg acgccgatgg tctcggttcc
     2701 ctcgtctgcg gccatgccac agcctaaccc gcgcgcagct gggcgcgctt gatcttgccg
     2761 ctgctcgtct tcggcagctc cgcggcgaac tcgacgatgc gggggaactt gtagggggcg
     2821 gtctcgcgct tgcagtgctc ctgcagctcg gcgaccaggg cgtcgctggc ctcgcgctcg
     2881 cgcaggacga cgatcgcgcg gacgacggcg ccgcgctcgg ggtcgggggc ggcgaccgcg
     2941 gcggcgtcgg cgacggcggg gtgggagagc agcgcggcct cgacctcggt cgggccgatc
     3001 cggtatccgg cggagaggat gatgtcgtcg ccgcggccct cgaagaagag gaagccgtcc
     3061 tcgtcttcgc ggaccaggtc accggtctgc caccactcgc cctcgaagcg ctcgccgtcg
     3121 aggtagccgg agaagaaggt gggggaggtg gcggcgcgga gctgcagttc gccgtcggcg
     3181 atccgggtct cgattccggg cagcggcttg cccatcgaac cgagccggat cggctcgccg
     3241 gcgaggttgg cggtcacctg gccggtctcg gtctggccgt agccgtcggc gggctcgagg
     3301 cccatcgcct cgcggaaggc gccgatcgtc tccgggtcga gcggctcgcc ggccgagacc
     3361 atgcggcgca gcgccggcag cggccgcagc gcggtgcgct tggccagcat ccggtactcg
     3421 gtcggcgcct ggcagagcac gttgacgccg agcacctcgg cgaactccag gcgctcggcc
     3481 gggtcgaagc ggccgtcgtg gatcaccgcg gcggcgccgg tcagccaggg ggcgaggaag
     3541 acgttgcggg ccgacttcga ccagccggtc gcggtcgtgc accaggccag atcgccgggt
     3601 cgcgagccga accagtgggt cgcctggacc tcctgtccga acaggtagcg gtagccgtgc
     3661 acgacgccgc gcgggttgcc ggtggtgccg gaggtgaaga cgatcagcgc cgggtcgtcg
     3721 gggtccatcg gctcgaccgc ggccggggtc tcctgcggct ggtcctcgtc gagcacgtag
     3781 gcgagttcct cgaggtccat ggtcgggatg ccctcgggca gctcggtcat cagctcggcc
     3841 tcgccgacac acagcttcgg ctccgcctcc gcggtccgca gttcgagatc gtggcgacgc
     3901 agcatcgtgt tgcagggcag cgccaccgcg cccatgcgcc agcaggcgag cagggtcagc
     3961 acccactcga tccggttgcc gaccatcgtc atcaccacgt cgcccttccc gaccccgcgc
     4021 gccgcgaagg caccggagag tccggccgaa caagcgatca gttcgccgaa gtgccagtcg
     4081 cgccggttgg cctcggcgtc gacggcgacg atccccagcc gtgaggctgg ggatcgctcc
     4141 atcaagtcct cgatcaggtt cacggaccga ggattatccc tggctaccgc aattcgctgc
     4201 tgctcttgcc gagcagctga cgggcgacga tcagcagctg gatctgctgg gtgccctcga
     4261 agatgtcgag gatcttcgag tcgcgggccc acttctcgag cagctcgttc tcgccgtagc
     4321 cgacgctgcc gcagagctcg acgcagccga gcgtcacctc gacgcaggtg cggccggcct
     4381 tcgccttcgc catcgaggcc tgcagcgagt tcggcttcga gttgtcggcc atccaggcgg
     4441 cgtgcatggt gagcagccag gaggcctcgt agtcggcctc catttcgagg tagcgggccg
     4501 cggccgcgtg ttgggagagg gcgggtttgt cgtagtcgat ctcgactccg gcctcgctca
     4561 gtcgccggcg ggtctcctcg agcgccgccc gggtcacccc gaccgccatc gcggcgacca
     4621 gcggccgcgt gttgtcgaac gtttccatca cgcccgcgaa gcccttctcc ttgacctcgg
     4681 ggtcgccgag cagcgcctct ttcggcaccc ggcagtcctg cagggtgaag ttggcggtgt
     4741 cggaggcgcg gatcccgagc ttgtgctcca gacggtccaa tttcaggccg gggttgtcgc
     4801 gctcgacgat gaacgacttg atcgccgccc gccctttgct gcggtcgagc gaggcccaga
     4861 ccacaatcag gtcggcgcgg tcgcccgaag tgacgaagat cttctctccg ttcaggacgt
     4921 actcgtcgcc gtcgagctcg gcggtggtgc ggatcgcggc cgagtccgag ccggcttccg
     4981 gctcggtgat cgccatcgcc gcccacttgc cgccgtagtg ctcgagctgc tgctcggtcg
     5041 cgaccgaggc gatcgccgag ttgccgagcc cctggcgcgg catcgagagc agcaggccga
     5101 cgtcgcccca gcagagctcg atgatcccga gcacggtgga gaggttggag ccgttgcggt
     5161 tctcgccgtc gtcgctcttc tcgcggcgca cgccggcggc gccggcgccg ccgatgtcgg
     5221 agccctcgtt catgccgtcg atcagcgagg cgagcatgtc cagctccttc gggtactcgt
     5281 gctcggcctc gtcgtacttg cgcgagatcg gccggaagac ctcgttcgcg acctggttgg
     5341 cctgggttgc gagcggtttg aacttcttcg gtatctcgag gttgatcatg gttgcctttc
     5401 gctcgggcgg gccgttagac gagcagggcg ccctccatca cgcccgcagc tcggaggtct
     5461 cggtaccagc gctcgaccgg gtgctccttg acgaagccgt ggccgccgag cagctggacg
     5521 ccgtcggtgc cggccttcat cgccttggcc gtgcactgga cgcggaccag gccggcctcg
     5581 cgcgagatcg gacggccgtg atcggcgagc gcggcggcgc ggtggacgag caggcgcatg
     5641 ccctcgagct cgatcgcgag gtcggcgatc aggaacgcca ccgactggcg gtgggtgatc
     5701 ggctcgccga acgccttgcg gtcgttgcag tacgggatca cgtagtcgag gacggcgcgg
     5761 gcgccgccga
//
LOCUS       DASRIG010000418         6361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_434796, whole genome shotgun sequence.
ACCESSION   DASRIG010000418 DASRIG010000000
VERSION     DASRIG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434796"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VFB52_11155"
     CDS             <1..1815
                     /locus_tag="VFB52_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06936.1"
                     /translation="TLGVIAVVVFHWAITTKHALDRGMFNFDTLWYHQPFSVDMVQSH
                     SLTGMWHVETVFTNWFYPQNSELLHAVGIALTERDTLSLFLNFGWLAVAFLAAWGIGR
                     PYGKGHLSVVAVAILLECHTLVVREPGAAKNDLMAAALFLAAIAILVELWSRRERLAP
                     GWPLAAAGLAVGLAAGTKVTVLAMAAALSVVVLVLAPRGRRWAAAGWWFVPALLGGGF
                     WYLRNLVVAGNPIPAIESIGPISLPHPERLQIGRPDFNIAHYATDTGVWSEYFTPGLH
                     DAFGVLWPLVLGGALAAALWALLAGRDRVLRGAGAVALFGMLAYLFTPLSAAGAEGEP
                     VGFAINVRFVVPALLLGLALLPLPRALDAPRRQWGLLGALVLVFLLTDRPDEALRDPS
                     RPFALLLVVLLVAIPAALLRARQRGAGRGIVLGAVAALLVAVLAIGYPLQRDYLDARY
                     ANDGPEDKQIPGMDLNSSYRWARGVEDARIGLAGTTAGFAGYGFYGTDLSNEVIYLGD
                     PGPHGAFNAIPTCAGFRAAVNAADLDYLVTSPFLNFLHPDDPVSSPEARWLRGEDAVA
                     PLLRSGPVTVWRVEGRLSPAECDGANAPLVEIPDTPSS"
     gene            1848..2171
                     /locus_tag="VFB52_11160"
     CDS             1848..2171
                     /locus_tag="VFB52_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06937.1"
                     /translation="MAYPLENALFQWEEGWRALQALTDPRERRLADRVIGAIRDELRR
                     RIGPTFSAAELADLYGAGTDWAQQVALDVAPAAEGDAQSLADAAFWLYLRGATDFAGG
                     RQLPV"
     gene            complement(2175..2768)
                     /locus_tag="VFB52_11165"
     CDS             complement(2175..2768)
                     /locus_tag="VFB52_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06938.1"
                     /translation="MPRRSLKPQLNQIRAWVHQGRTDAWIAHQLEVSASELRDFRKQY
                     DLSPDDENAGSSEIDLRDEIEAEIEAAAEQEASEEAERAERGEDDEDSEGDDDDDEDG
                     EGSGGSKPRRRRRRGRRGGRGRRKEIEATFDHGETDGYGLWLDAAIKDNPVYAEHWAG
                     KRDVTVRIEADQIVIRRAGAGGGGSEESDSESDSDDE"
     gene            complement(2821..3054)
                     /locus_tag="VFB52_11170"
     CDS             complement(2821..3054)
                     /locus_tag="VFB52_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06939.1"
                     /translation="MRQSISHFEAAFEQEATLERRRREQLRRRAANRSRARKITRVEQ
                     QGKVRFSVLAVCLTVTVIVVVVAMFEALALLMA"
     gene            complement(3124..3501)
                     /locus_tag="VFB52_11175"
     CDS             complement(3124..3501)
                     /locus_tag="VFB52_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZO06940.1"
                     /translation="MDGDTIEVRLDGEIEDVRYIGVDTPETVKPGSPVDCFGPQASHF
                     NHRLVEGRRVRLVFGVERRDVYGRLLSYVYLGPRFVNAELLRRGLARTLTIAPNDRFS
                     ERFKRLEIAAGRAGRGLWSACES"
     gene            complement(3692..4612)
                     /locus_tag="VFB52_11180"
     CDS             complement(3692..4612)
                     /locus_tag="VFB52_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06941.1"
                     /translation="MELKSLTEQKSLLAVLPPGKRARLHRLLFEFGPGNGTLMLLPID
                     QGIEHGPRDFFPNPASKDPDYQFRLAAEAGYSALACQIGMAEKYYPDYAGRVPLILKV
                     NGKTDIPPSSASFSSCNASVEDGVRLGADAIGYTLYVGSPRQDEDLAQLHGVREECDR
                     YGMPLVIWSYPRGEFVDAKGGANSFYAIDYAARMAMEMGADVVKLNMPKIDAAKDKDA
                     PAPYNEMEVDQAEAIRQCVESAGRALVVLSGGSKTDDETVLNHTREVMEAGGSGVIFG
                     RNVWQREWNEAQGIIEQIKETLLANVRRTP"
     gene            4702..5463
                     /locus_tag="VFB52_11185"
     CDS             4702..5463
                     /locus_tag="VFB52_11185"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZO06942.1"
                     /translation="MSETELSVICKNCGSEVSPYVTECPYCGARLRKRAPKLERRGDA
                     LEAQKPRRRRRLLPRRPAFLVLDRPYATLVVIFGSAIMLLLQEATGYELVSFGGLMFP
                     FADEWWRYLTAPFAYADVGYLFAIALALIIFSSGVERRLGTVTTAILLVACGSLGALA
                     AGAVANQQDGLTLVSGGNGIALGMIAAWFALRRAEARGAIDEEYDAIGVAVCAVAILL
                     LPLFVYDADPIAGVAGGLVGGLAGLLAARLRPSHP"
     gene            5488..5805
                     /locus_tag="VFB52_11190"
     CDS             5488..5805
                     /locus_tag="VFB52_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06943.1"
                     /translation="MPDESDFEAAIERLLDPERFSEAERIVARAAPQLQKVLAAALAE
                     GGWFGEPHEAEALRAATVPDPDERITAVRALLAEEARMGMMVGVAVGWALREEMSDPN
                     GES"
     gene            5806..>6361
                     /locus_tag="VFB52_11195"
     CDS             5806..>6361
                     /locus_tag="VFB52_11195"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZO06944.1"
                     /translation="MEIKYHGHSCFELSDGETTLLIDPFLKPNNPVAVATAEEINPTH
                     IAITHGHADHMADAVPVAKRTGAHCVSIVELAEWLKGQGVEEVSDPNLGGTVRFDWGS
                     IKLVPAWHTSTLPGSEEAPFSAEHGIAIGTPSGFVIKLGGLTIYDAGDTCLFGDMKLI
                     GELEQPDIALLPIGGHYTMDRHDAV"
BASE COUNT          860 a         2269 c         2191 g         1041 t
ORIGIN      
        1 acgctcggcg tcatcgcggt cgtggtgttc cactgggcga tcaccacgaa gcacgccctc
       61 gaccgaggga tgttcaactt cgacaccctc tggtaccacc agcccttctc cgtggacatg
      121 gtccaaagcc actccctcac gggcatgtgg cacgtcgaga ccgtcttcac caactggttc
      181 tacccgcaga actccgagct cctgcacgcg gtcgggatcg cgctgaccga acgcgacacg
      241 ctctcgctct tcctcaattt cggctggctc gcggtcgcct tcctcgccgc ctggggaatc
      301 ggcaggccgt acggcaaggg ccacctgagc gtggtcgcgg tggcgatcct gctcgagtgc
      361 cacaccctgg tcgtgcgcga gcccggcgcc gccaagaacg acctgatggc cgccgccctc
      421 ttcctcgccg caatcgcgat cctggtcgag ctctggagcc ggcgcgagcg gctggcgccg
      481 ggctggccgc tggcggcggc ggggctggcc gtcgggctgg cggcggggac caaggtgacg
      541 gtgctggcga tggcggcggc gctgagcgtc gtcgtgctgg ttctggcgcc gcgtgggcgg
      601 cgctgggcgg cggcgggctg gtggttcgtg ccggccctgc tcggcggcgg cttctggtac
      661 ctgcgcaacc tcgtcgtcgc cggcaacccg atccccgcga tcgagagcat cgggccgatc
      721 tcgctgcccc accccgagcg tctgcagatc ggccgtcctg acttcaacat cgcccactac
      781 gcgaccgaca ccggcgtctg gagcgagtac ttcaccccgg gcctgcacga cgccttcggc
      841 gtgctctggc ccctcgtcct cggtggcgcc ctcgcagcgg cgctctgggc gctgctggcg
      901 ggccgtgacc gggtcctgcg gggggcgggg gcggttgccc tcttcggcat gctcgcctac
      961 ctgttcacgc cgctcagcgc cgccggcgcc gagggcgaac cggtcggctt cgcgatcaac
     1021 gtccgcttcg tcgtccccgc cctgctgctc ggcctcgccc tgctgccgct gccgcgggcg
     1081 ctcgacgcgc cgcgccgcca gtgggggctg ctcggagccc tggtgctcgt cttcctgctc
     1141 accgatcgtc ccgacgaggc gctcagggac ccctcgcggc cgtttgcgct gctcctggtg
     1201 gtcctgctgg tggcgatccc ggcggccctc ctccgcgcgc gccagcgtgg tgccggccgt
     1261 gggatcgtgc tcggcgccgt cgcggcgctg ctcgtcgcgg tgctcgcgat cggctatccg
     1321 ctccagcgcg actacctcga cgcccgctac gccaacgacg gccccgagga caagcagatc
     1381 cccggcatgg acctcaactc ctcctaccgc tgggcgcgtg gggtggagga tgcccgcatc
     1441 ggcctcgccg gtaccaccgc gggcttcgcc ggctacggct tctacggcac cgatctctcc
     1501 aacgaggtca tctacctcgg cgatcccggc ccgcatggcg ccttcaacgc gatcccgacc
     1561 tgcgccggct tccgcgccgc cgtcaacgcc gccgacctcg actacctggt cacctcgccc
     1621 ttcctcaatt tcctccaccc cgacgacccg gtcagctcgc ccgaggcgcg ctggctgcgc
     1681 ggggaggacg cggtcgcgcc gcttctgcgc agcggcccgg tcacggtctg gagggtcgag
     1741 ggcaggctca gcccggcgga gtgcgacggc gccaatgcgc cgctcgtcga gattccggac
     1801 acgccctcgt cctgagtcgg cgccgccggc tttgtaatct cgccgggatg gcctatccgc
     1861 tcgagaacgc gctcttccag tgggaggagg gctggcgcgc cctgcaggcg ctcaccgacc
     1921 cgcgcgagcg ccggctcgcc gaccgggtga tcggggcgat ccgcgacgag ttgcggcgcc
     1981 ggatcgggcc gaccttcagc gccgccgagc tggccgacct ctacggcgcc ggcaccgatt
     2041 gggcgcagca ggtcgcgctc gacgtcgcgc cggcggccga aggcgacgcc cagtcgctgg
     2101 ccgacgccgc cttctggctc tacctacgcg gggcgactga cttcgcgggc gggcgccagc
     2161 tgccggtcta agcgctactc gtcgtcggag tccgactcgg agtcggactc ctccgagcca
     2221 ccgccacccg caccggcgcg gcggatcacg atctggtcgg cctcgatgcg gacggtgacg
     2281 tcgcgtttgc cggcccagtg ctcggcgtag acggggttgt ccttgatcgc ggcgtcgagc
     2341 cagaggccgt agccgtcggt ctcgccgtgg tcgaaggtcg cctcgatctc tttgcggcga
     2401 ccgcggccgc cgcggcgacc gcgacggcgg cgccggcgcg gtttcgaacc gccggagccc
     2461 tctccgtcct cgtcgtcgtc atcatcgccc tcggagtcct cgtcgtcctc gccccgctca
     2521 gcccgttcgg cttcttcgct ggcttcctgc tcggcagccg cctcgatctc cgcttcgatc
     2581 tcgtcgcgca gatcgatttc gctcgatccg gcgttctcgt cgtcggggga taggtcgtac
     2641 tgcttgcgga agtcgcgcag ctcggaggcg ctgacttcga gttggtgggc gatccaggcg
     2701 tccgtgcggc cttggtggac ccaggcgcgg atctgattca gttgcggctt cagtgagcgt
     2761 cgtggcatag gcgcgcgagt ctattcaacc ggggggttct gccgcgggta gcccgggggg
     2821 ctaggccatc agcagggcga gcgcctcgaa catcgccacc acgaccacga tcacggtcac
     2881 ggtcagacag acggcgagaa cgctgaagcg aaccttgccc tgctgctcga cgcgggtgat
     2941 tttgcgagcc cgagagcggt tggcggcgcg gcgacggagc tgctcgcggc gacgcctctc
     3001 gagggtggcc tcctgctcga aggcggcctc gaagtgggag atgctttgac gcattcgcat
     3061 aattagggcc cccgaggggc cgcgggcacc gtagcaaagg atcttgcaga actcacgtga
     3121 agctcacgat tcacacgcac tccagagtcc ccttccggcc ctcccagccg caatctcaag
     3181 ccgtttgaag cgttctgaaa agcggtcgtt gggagcgatc gtgagcgttc gcgcaagtcc
     3241 tctccgcagt agttccgcgt tgacgaagcg ggggccgagg tagacataag aaagcaggcg
     3301 cccgtagacg tcgcgccgct cgaccccgaa gaccagccgc acccgccggc cctcgaccag
     3361 gcggtggttg aagtgcgagg cctgcggccc gaagcaatcg accggggagc ccggcttcac
     3421 cgtctccggc gtgtcgacgc cgatgtagcg gacatcctcg atctcgccgt cgagccggac
     3481 ctcgatcgtg tctccgtcca ccacccgcgt gacctgcacc caggcgctcg ccggtccgcc
     3541 ttctccaccg gacgcgtccc agggtgggct ctcccagggc cgcaacaaaa gaagcgcggc
     3601 cgcaaccaac acgaccgcgc ttccaagagt ccccctcatg cccggctaag ggcggagggc
     3661 acgtgttctg cccccatcgg ggccggcgtc cctacggggt gcgccgcacg ttggcgagca
     3721 gcgtctcttt gatctgctcg atgatgccct gggcctcgtt ccactcgcgc tgccagacgt
     3781 tgcggccgaa gatcacgccg gagccgcccg cctccatcac ctcgcgggtg tggttgagca
     3841 cggtctcgtc gtcggtcttc gagccgccgg agagcacgac cagggcgcgg ccggccgatt
     3901 cgacgcactg gcggatcgcc tccgcctggt cgacctccat ctcgttgtac ggggccgggg
     3961 cgtccttgtc cttggccgcg tcgatcttcg gcatgttgag cttgaccacg tcggcaccca
     4021 tctccatcgc catccgcgcc gcgtagtcga tcgcgtagaa ggagttggcg ccgcccttgg
     4081 cgtcgacgaa ttcgccgcgc gggtaggacc agatcaccag cggcatcccg tagcggtcgc
     4141 attcctcgcg cacgccgtgc agctgggcga ggtcctcgtc ctggcgcggg gagccgacgt
     4201 agagcgtgta gccgatcgcg tcggcgccga gccggacgcc gtcctcgacg ctggcgttgc
     4261 aggaggagaa ggaggccgac gacggcggga tgtcggtctt gccgttgacc ttgaggatca
     4321 gcggcacccg gccggcgtag tcggggtagt acttctccgc catgccgatc tggcaggcga
     4381 gggccgagta gccggcctcg gcggcgagcc ggaactggta gtcggggtcc ttggaggcgg
     4441 ggttggggaa gaagtcacgc ggaccgtgct cgatcccctg gtcgatcggc agcagcatca
     4501 gcgttccgtt gcccgggccg aactcgaaca gcagccggtg caggcgggct cgtttgcccg
     4561 ggggcagcac cgcaagcagg gatttctgct cggtcagact cttcagctcc acagtcaatt
     4621 cctcctcgaa gattggggtc ttgggagtat ggcaccgcaa acgcgggcag agccctccac
     4681 gcgaggggta ttctcggtgt catgagcgag accgagctca gcgtcatctg taagaactgc
     4741 ggctcggagg tcagtcctta cgtgaccgag tgcccctatt gcggggccag gttgcgcaag
     4801 cgggccccga agctggagcg ccggggtgat gccctggagg cacagaagcc acgccgtcgg
     4861 cgtcggctgc tgccgcgacg cccggccttc ctggtcctcg accgccccta cgcgacgctg
     4921 gtcgtgatct tcggcagcgc gatcatgctg ctgctccagg aggcgaccgg atacgagctg
     4981 gtcagcttcg gcggcctgat gttccccttc gccgacgagt ggtggcgcta cctgaccgcc
     5041 cccttcgcct acgccgacgt cggctacctg ttcgcgatcg ccctggccct gatcatcttc
     5101 tccagcgggg tcgagcgccg gctcggcacg gtgacgaccg cgatcctgct ggtcgcctgc
     5161 ggctcgctcg gggcgctggc ggcgggcgcc gtcgccaacc agcaggacgg cctcaccctg
     5221 gtctcgggcg gcaacggcat cgcgctcggg atgatcgccg cctggttcgc gctgcgccgc
     5281 gccgaggcgc gcggggcgat cgacgaggag tacgacgcga tcggggtcgc ggtctgcgcg
     5341 gtcgcgatcc tgctgctgcc gctgttcgtc tacgacgccg atccgatcgc cggcgtggcc
     5401 ggcggcctgg tcggtgggct ggccgggctg ctcgccgcgc gcctgcggcc ctcgcacccg
     5461 taagcggccc ccgttaggct ccgccgggtg ccggacgaga gcgatttcga agccgcgatc
     5521 gagcggctgc tggaccccga gcgattctcc gaagccgagc ggatcgtcgc ccgcgcggcg
     5581 ccgcagctgc agaaggtgct ggccgcggcc ctcgccgagg gcggctggtt cggcgagccg
     5641 cacgaggcgg aggcgctgag ggccgcgacc gttcccgatc ccgacgagcg gatcaccgcc
     5701 gtgcgcgcgc tgctcgccga ggaggcgcgg atggggatga tggttggcgt cgccgtcggt
     5761 tgggcactga gagaagagat gagcgacccg aatggagaga gctagatgga gatcaagtac
     5821 cacggtcatt cctgcttcga gctcagcgac ggcgagacga cgctgctgat cgacccgttc
     5881 ctgaagccga acaaccccgt cgcggtcgcg accgccgagg agatcaaccc gacccacatc
     5941 gcgatcaccc acggccacgc cgaccacatg gccgacgcgg tgccggtggc gaagcggacc
     6001 ggcgcccact gcgtctcgat cgtcgagctc gccgaatggc tgaaggggca gggggtcgag
     6061 gaggtcagcg accccaacct gggtggcacc gtgcgcttcg actggggctc gatcaagctc
     6121 gtccccgcct ggcacacgag caccctgccg ggctccgaag aggcgccgtt cagcgccgag
     6181 cacgggatcg cgatcggcac cccgagcggc ttcgtgatca agctcggcgg cctcaccatc
     6241 tacgacgccg gcgacacctg cctgttcggc gacatgaagc tgatcggcga gctcgagcag
     6301 ccggatatcg ccctgctgcc gatcggcggc cactacacga tggaccgcca cgacgccgtc
     6361 c
//
LOCUS       DASRIG010000419         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1509575, whole genome shotgun sequence.
ACCESSION   DASRIG010000419 DASRIG010000000
VERSION     DASRIG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509575"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFB52_11200"
     CDS             <1..217
                     /locus_tag="VFB52_11200"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZO06945.1"
                     /translation="LCSGPGKLTEALGVTLSENDADLGREPFLVLPPEGEPLEVVTGP
                     RIGITKAVDRPWRFCAADNEFVSKPWP"
     gene            complement(223..2514)
                     /locus_tag="VFB52_11205"
     CDS             complement(223..2514)
                     /locus_tag="VFB52_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZO06946.1"
                     /translation="MAFFRRKRRQDGGGDIPPAPPAPQKRKRPRLKKLRILFVLLGLG
                     ILALVSTVFGMMAAVSQDLPAIYNFAQYKSSKNSVLVDANGEMLGTLTSDENKILLTS
                     GQISPNMKNAVVAVEDARFYEHDGVDFQGIGRALVKDILSMSAQQGGSTITQQFVKQA
                     LDAAGDRSLFVKFKEAALSYRLEQHWSKDKILTEYLNTVYFGEGAYGIEAAARTYFGD
                     AHPGCGTETEPCASVLEPWEAAMLAGIIQSPSAYDPKYFPENALMRRNVVLEKMSEQG
                     YISSQQFVEGKKQALPAPNAIQPPTLDAKAPYFTAFVRQQLLERYGPSKAFFGGLRVK
                     STLDLRLQEKAEEAVNGYMGYTPATASVVVIDNNNAGIKAMVGGPDFAEKPFNLATQG
                     HRQPGSSIKPFILLTALEQGISPTTTYESAPQSFRFGKKDKEIFEVTNDEDLYAGVSS
                     IESATTNSDNSVYAQIALESLKGKTIHDRTASIAATAHKMGYNDKLSTNPAMVLGGLD
                     PGVTPLGWTYAFSTLGNNGDRVSGTLAPRPGKSPVAYTEVTDGDGDVIKGGDNDSTHK
                     QVLDENVALEAKRILETVVSSGTGTNADTGAEGEWGKTGTTENNGDAWFCGGVQDEVT
                     ACVWVGYADSVTPMETLYQGGPVMGGTFPALIWASVINAWEEIQAEKAAEKAARKAER
                     EAKKEAEENGEDFDAGEYEYVPTEPTEETYEVEGGGEGAVEPEEVTPEETPEEGAAEE
                     ATPEETPEATPEGGGGGGGVTAG"
     gene            2589..3752
                     /gene="tyrS"
                     /locus_tag="VFB52_11210"
     CDS             2589..3752
                     /gene="tyrS"
                     /locus_tag="VFB52_11210"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZO06947.1"
                     /translation="MSDLTQNAVDVLPAGRLAEQLAGDRPLRVKLGIDPTTADIHLGH
                     TVVLEKLAAFQRAGHTVVLIIGDFTARVGDPSGRSATRPVPSAEEIEANAATYQEQAF
                     KLLDRERTEVRYNSEWLRMEPEGLLGLLAQTTVARLLERDDFQKRMAANQPIAALELL
                     YPLLQGYDSVAIEADVELGGTDQKFNLLFGRDIQTAYGQAPQSILTMPILVGTDGVQK
                     MSKSLGNYVGVTDPPEEMFGKLMSIPDSAMDEYFRLLLGSETPAGPPNEAKRELGRRI
                     VDRFHGEGAGAAAEAHFNQVFVDRGVPDEIPDWDISPYGGSEAGVILLPQLIGAGFEL
                     SSSEARRLIKQGGVKLDGEPVAADALELPRAELAGKVLQVGKRRFLQLVDGAA"
     gene            4094..>4506
                     /locus_tag="VFB52_11215"
     rRNA            4094..>4506
                     /locus_tag="VFB52_11215"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          682 a         1432 c         1549 g          843 t
ORIGIN      
        1 cctctgctct ggtcccggca agctgaccga ggcgctcggc gtcaccctct ccgagaacga
       61 cgccgacctc ggccgcgagc ccttcctggt tttgccgcca gagggggagc cgcttgaggt
      121 cgtcaccggc ccccggatcg ggatcaccaa agcggtcgac cggccctggc gcttctgcgc
      181 cgccgacaac gagttcgtct cgaaaccctg gccgtagcgg gctcagccgg cggtcacgcc
      241 gccgccccca ccgccgcctt cgggcgtggc ttcgggcgtt tcttccgggg tggcttcttc
      301 ggccgcgcct tcttccggcg tttcttcggg cgtgacttct tcgggttcga ccgcgccttc
      361 gccgccgcct tcgacttcgt aggtttcttc ggtcggttcg gtcggcacgt attcgtattc
      421 gccggcgtcg aagtcttcgc cgttctcttc ggcttccttt ttcgcctccc gttcggcttt
      481 gcgcgcggcc ttttcggctg ccttttcggc ctggatctct tcccaggcgt tgatcacgct
      541 ggcccagatc agggccggga aggtgccgcc catcaccggg ccgccctggt agagcgtttc
      601 catcggcgtg accgagtcgg cgtagccgac ccagacgcag gcggtcacct cgtcctggac
      661 gccgccgcag aaccaggcgt caccgttgtt ctcggtggtg cccgtctttc cccactcgcc
      721 ttcggcgccg gtgtcggcgt tggtcccggt gccgctggag acgaccgttt cgaggatccg
      781 cttggcttcc agcgccacgt tttcgtcgag gacctgtttg tgggtggagt cgttgtcgcc
      841 gcctttgatc acgtcgccgt cgccgtcggt gacttcggtg taggccaccg ggcttttgcc
      901 ggggcgcggc gccagcgtgc cgctgacccg gtcgccgttg ttgccgaggg tcgagaaggc
      961 gtaggtccag ccgagcggcg tgaccccggg gtcgaggccg ccgaggacca tcgcggggtt
     1021 ggtcgaaagc ttgtcgttgt agcccatctt gtgggcggtc gcggcgatcg aggcggtgcg
     1081 gtcgtggatc gtcttgccct tcagactctc gagggcgatc tgggcataga ccgagttgtc
     1141 ggagttggtc gtcgccgatt cgatcgagct gacccccgcg tagaggtcct cgtcgttggt
     1201 gacttcgaag atttccttgt cttttttgcc gaagcggaag ctctgcgggg ccgattcata
     1261 ggtggtcgtc ggcgagattc cctgttcgag cgcggtcagc aaaatgaacg gcttgatcga
     1321 ggagcccggc tggcggtggc cctgggtcgc caggttgaac ggcttttcgg cgaagtcggg
     1381 accgccgacc atcgccttga tcccggcgtt gttgttgtcg attaccacca ccgaggcggt
     1441 cgccggcgtg tatcccatgt agccgttgac cgcttcttcg gccttttcct ggaggcgcag
     1501 gtcgagcgtc gacttgaccc gcaagccgcc gaagaaggcc ttcgaggggc cgtagcgttc
     1561 gagcagctgc tggcgcacga aggcggtgaa gtagggggcc ttggcgtcga gcgtcggcgg
     1621 ctggatcgcg ttcggggccg gcagcgcctg ttttttgcct tcgacgaact gctggctgga
     1681 gatgtagccc tgttcggaca tcttttccag caccacgttg cgccgcatca gggcgttttc
     1741 ggggaagtat ttggggtcgt aggccgaggg cgactggatg atcccggcca gcatcgccgc
     1801 ctcccagggc tccagcaccg aggcgcaggg ctccgtttcg gtgccgcagc cggggtgggc
     1861 gtcgccgaag taggtgcggg cggcggcctc gatcccgtag gcgccctcgc cgaagtagac
     1921 cgtgttcagg tactcggtga ggatcttgtc cttggaccag tgctgctcga gccggtagga
     1981 gagcgccgcc tccttgaatt tgacgaacag cgagcggtcg ccggcggcgt cgagcgcctg
     2041 cttgacgaac tgctgggtga tcgtcgagcc gccctgctgg gcactcatgc tgaggatgtc
     2101 tttgaccagg gcgcggccga tgccctggaa gtcgacgccg tcgtgttcgt agaagcgcgc
     2161 gtcctcgacc gcgaccaccg cgttcttcat gttcggcgag atctggcccg aggtgagcag
     2221 gattttgttc tcgtcgctgg tcagcgtgcc gagcatttcg ccgttggcgt cgaccaggac
     2281 gctgtttttc gaggacttgt actgggcgaa gttgtagatc gccggcaggt cctgggagac
     2341 cgcggccatc atcccgaaca cggttgagac cagggcgagg atcccgagtc cgagcagcac
     2401 gaagaggatc cgcagcttct tcaggcgcgg acgcttgcgc ttctggggag ccggtggagc
     2461 gggcggaatg tctccgccgc catcctgccg gcgctttctt ctaaagaagg ccatcgaggg
     2521 ggtgaaacac aaacgcccga cgaccagatc ggttcgccga gcggaggcgg cagtctagca
     2581 ttgccgccat gtcggatctg acgcaaaacg ccgtcgacgt cctgcccgcg gggcgcctgg
     2641 cggagcaact ggccggtgac cggccgctgc gcgtcaagct cgggatcgat ccgaccaccg
     2701 ccgacatcca cctcgggcac acggtcgtgc tcgagaagct ggccgctttc cagcgcgccg
     2761 ggcacaccgt cgtcctgatc atcggcgact tcaccgcccg ggtcggcgac ccgagcgggc
     2821 gctcggcgac ccggcccgta ccgagcgctg aggagatcga ggcgaacgcc gcgacctacc
     2881 aggagcaggc gttcaagctg ctcgatcgcg agcgcaccga ggtccgctac aacagcgagt
     2941 ggctgcggat ggagccggag ggcctgctcg ggctgctcgc ccagaccacg gtcgcccgcc
     3001 tgctcgagcg cgacgacttc cagaagcgga tggcggcgaa ccagccgatt gcggcgctgg
     3061 agctgctcta cccgctgcta cagggctacg actcggtcgc gatcgaggcc gacgtcgagc
     3121 tgggggggac cgaccagaag ttcaacctct tgttcggccg cgacatccag accgcctacg
     3181 ggcaggcgcc gcagtcgatc ctgacgatgc cgatcctggt cggcaccgac ggcgtgcaga
     3241 agatgagcaa gtcgctcggc aactacgtcg gcgtcaccga cccgccggag gagatgttcg
     3301 gcaagctgat gtccatcccc gactcggcga tggacgagta cttccgcctg ctgctgggga
     3361 gcgagacgcc ggcgggcccg ccgaacgagg ccaagcggga gctcgggcgc cggatcgtcg
     3421 accgcttcca cggcgagggg gcgggggcgg ccgccgaggc ccacttcaac caggtcttcg
     3481 tcgatcgcgg cgtgcccgac gagatccccg actgggacat ctccccctac ggcggcagcg
     3541 aggccggcgt gatcctgctg ccgcagctga tcggcgccgg cttcgagctc agctcgagcg
     3601 aggcacggcg gctgatcaag cagggcgggg tcaagctcga cggcgagccg gtggccgccg
     3661 acgcgctcga gttgccacgc gccgagctcg ccggtaaggt gctccaggtc ggcaagcggc
     3721 gcttcctgca gctggtcgac ggcgccgcct gaggcgtcgg gctccggcaa aagagcctgc
     3781 agagccaaac cggaatttct tcccgagcgg ccttgcaaac gttcgtggga gcgctatact
     3841 cccccgtcct tctggggctc ccttgtgggg ccccggcgtc tgagagcgac gagtcccgtc
     3901 gcggaggcgc aacggtcttt gaaaactgag cagcgtgcac tccgcggacc catcaacagg
     3961 tctgcggaat gtttgagccc gaaccatcgg tgcgccagcg cgccgatggt cttgatttac
     4021 ctcgtcatac cggccgtcgt acttgtgtac tcggcggcca tataggcatg acaggctctc
     4081 tgtagaagta catttcacgg agagtttgat cctggctcag gacgaacgct ggcggcgtgc
     4141 ttaacacatg caagtggtgc gacgaaccag ggcttgccct ggggcaaagc cgcgaacggg
     4201 tgagtaacac gtgggtaatc tgccccgatg accgggacaa cccgaggaaa ctcgggctaa
     4261 taccggatgt ggtgcacgga cataagtctg tgtactaaag gaagcttcgg cctccgcatt
     4321 gggaggtgcc cgcggcccat tagcttgttg gtggggtaac ggcctaccaa ggcgacgatg
     4381 ggtagctggt ctgagaggac gatcagccac actgggactg agacacggcc cagactccta
     4441 cgggaggcag cagtggggaa tcttgcgcaa tgcgcgaaag cgtgacgcag caacgccgcg
     4501 tggggg
//
LOCUS       DASRIG010000420         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_435780, whole genome shotgun sequence.
ACCESSION   DASRIG010000420 DASRIG010000000
VERSION     DASRIG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435780"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFB52_11220"
     CDS             complement(<1..525)
                     /locus_tag="VFB52_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06948.1"
                     /translation="MSTALLEATEHQGRARIALTAALESGPSHAYLFRGPRGAGKRAA
                     ARALAAEILADGAADPEDARRRALLDPSPHPDLVWIAPRGAQHMVEDVRERVIRAAAY
                     RPFEGGKRVFVIEAAEALRDESQNALLKTLEEPPPYAHIVLLTSEPEILLETIASRCA
                     PVEFAPYPAEVLEAE"
     gene            complement(506..1099)
                     /gene="tmk"
                     /locus_tag="VFB52_11225"
     CDS             complement(506..1099)
                     /gene="tmk"
                     /locus_tag="VFB52_11225"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZO06949.1"
                     /translation="MFISLEGIDGSGKSTQAKLLVEALGPETVAIREPGGTDAAERIR
                     TLLADGDVPLDPLAELLLFLAARADLTERVIRPALEAGHDVVSDRFSDSSVAYQGAAR
                     GLGVGEVISLCDTATDGLWPDLTLLLKVDPELGLGRAESDDRFEAEGLDLQRQVATAY
                     EEIAIIASDRVVAIDATGTVEEVHARVMEAVDVHRPA"
     gene            complement(1112..3436)
                     /locus_tag="VFB52_11230"
     CDS             complement(1112..3436)
                     /locus_tag="VFB52_11230"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HZO06950.1"
                     /translation="MRLIVTEKNSSAKKIAEILGAPTGGPKEDKTFKVPFYSWSDSDG
                     DHMTVGLKGHVLGPAFPEGYSNWQKTDLHDLIDAKLIKEPTDKNVVKAIRKLAKGADE
                     LVIATDFDREGELIGLEALEEMLDANPELGTREETEAGTLVVQRARYSALTKEEIERA
                     FGEIDTLSYPLANAGAARQDIDLLWGATLTRAVSLASRRFGSNFLSVGRVQSPTLGLI
                     VEREMERRAHVPKPFWELFAKFEHPDGSFETHHTVDKFWEKAEADKAFAGTKGPGVVK
                     SVTARKNTRKPPTPYNTTAFSTDASSRLGITPASAMRIAEDLYMDGFISYPRTDNTVY
                     PSSLPVRELISSLVRVKEFSAASGLLDGELTPTRGKKETTDHPPIYPTQAIHPGALDG
                     SKKRVYELVVRRFLATFSPPMITESTRADIEAGSETYFVRGSVVVDPGYAGIYTYARS
                     ADEEIPALQEGQSLELADVSSFAPSEDAPTENPWMVGKETPPPARISQAKLIEMMEER
                     GLGTKATRADIIQKLFDRGYVYGNPPVPTETGIALYEAFKNYVPAMATPEMTAQLEAE
                     MDRIAAGEMTKGAVVGESRELLHKTWTEIDGSREDLAKVIWKGMDEDRILGPCKVCEE
                     AGRKKEDGSPHMLRIIRAKKSGKRFVGCSGWVLDDPSSCDQTFPLPQRGDVFRLEERC
                     SICDQTPRLKVQPFRGRPWNLCLNDDCESMQEMKKRRAEREAAKKAKEAMDKKPPVKG
                     DEDAPAKKSKAKVKASKADTATRGRKRAKAATKS"
     gene            3538..4470
                     /locus_tag="VFB52_11235"
     CDS             3538..4470
                     /locus_tag="VFB52_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO06951.1"
                     /translation="MKTLVTGGAGFIGSNLVDGLLARGDEVTVVDDLSTGRLVNLEGA
                     LEAGAELVELDIRDAAGLTALVAERRPEAIFHLAAQIDVRKSLEDPAFDAAVNVGGTA
                     NVLEAARGAATERVVFVSTGGAIYGEGEGQDLPLDESTAIAPMSAYGQSKYAAEGYLA
                     LYERLYGLSTIALRLGNIYGPRQDPLGEAGVVAIFCGLVSSGGRPTVYGDGTQTRDYI
                     YVGDVVDAALAAADSRAGGAINIGTGREASVLDLVEILGQLGGAADFEPEFAPPRAGE
                     VQRISLDAALAERELGWSPQTSLEDGLRLTLDAI"
BASE COUNT          589 a         1518 c         1653 g          808 t
ORIGIN      
        1 ctcggcttcc agcacctcgg ccgggtaggg cgcgaactcg accggggcgc agcgcgaggc
       61 gatcgtctcc agcaggatct ccggttcgga ggtcaacagg acgatgtggg cgtagggcgg
      121 cggctcctcg agcgtcttca gcagcgcgtt ctggctctcg tcgcgcagcg cctccgccgc
      181 ctcaatcacg aagacccgct tgccgccttc gaagggccgg taggcggcgg cgcggatcac
      241 ccgctcacgg acgtcctcga ccatgtgctg ggcgccgcgc ggggcgatcc agaccaggtc
      301 gggatgcggc gaggggtcga gcagggcgcg gcggcgggcg tcctcggggt cggcggcacc
      361 gtcggccagg atctccgccg ccagcgcccg cgccgcagcg cgcttgccgg cgccgcgcgg
      421 cccgcggaag agataggcgt gcgagggccc gctctccagc gccgcggtca gcgcgatccg
      481 ggcgcgcccc tggtgctcgg tcgcctcaag cagggcggtg gacatcaacc gcctccatga
      541 cgcgggcgtg gacctcctcg acggtgccgg tggcgtcgat cgcgacgacg cggtcggagg
      601 cgatgatcgc gatctcctcg taggcggtgg cgacctggcg ctggaggtcg aggccctcgg
      661 cctcgaagcg gtcgtcgctc tcagcgcggc cgaggccgag ctcggggtcg accttgagca
      721 gcagcgtcag gtcgggccag aggccgtccg tagcggtgtc gcagagcgag atcacctcgc
      781 cgacgccgag cccgcgcgcg gctccctggt aggcgacgct ggagtccgag aagcggtctg
      841 agaccacgtc gtggccggcc tccagagccg gccggatcac ccgctcggtg aggtcggcgc
      901 gggcggcaag gaagagcagc agctcggcca gcgggtcgag cggcacgtca ccatcggcga
      961 ggagagtgcg gatccgctcg gcggcgtcgg tcccccccgg ctcgcggatc gccaccgtct
     1021 ccgggccgag cgcctcgacc agcagtttcg cctgggtgct cttgcccgag ccgtcgattc
     1081 cctcgagcga gatgaacaac gtccggcggc ctcagctttt ggtggcggct ttggcgcgtt
     1141 tgcgcccgcg cgtcgccgtg tccgctttcg aagctttgac tttcgctttc gatttcttcg
     1201 cgggcgcgtc ctcgtcgccc ttgaccggcg gtttcttgtc catcgcttct ttggcttttt
     1261 tcgccgcttc gcgctcggcc cggcgtttct tcatctcctg catcgactcg cagtcgtcgt
     1321 tgaggcagag gttccagggc cggccgcgga agggctggac tttcaaccgc ggggtctggt
     1381 cgcagatcga gcagcgctcc tcgaggcgga agacgtcacc gcgctggggc agcggaaagg
     1441 tctggtcgca ggagctcggg tcgtcgagca cccagccgct gcagccgacg aagcgtttgc
     1501 ccgatttttt cgcgcggatg atccgcagca tgtgcggcga gccgtcctcc tttttgcggc
     1561 cggcttcttc gcagaccttg caggggccga ggatgcggtc ctcgtccatc cctttccaga
     1621 tcacttttgc caggtcctcg cggctgccgt cgatttcggt ccaggtcttg tgcaggagct
     1681 cgcggctctc gccgacgacg gcgcccttgg tcatctcgcc ggcggcgatc cggtccatct
     1741 ccgcctccag ctgggcggtc atctccggcg tcgccatcgc cgggacgtag ttcttgaagg
     1801 cctcgtagag agcgatcccg gtctccgtgg gaaccggcgg gttgccgtag acgtagccgc
     1861 ggtcgaacag cttctggatg atgtcggcgc gcgtggcttt ggtgccgagg ccgcgctcct
     1921 ccatcatctc gatcagtttc gcctgggaga tccgggccgg cggcggggtt tccttaccga
     1981 ccatccaggg attttcggtc ggcgcgtcct cactcggcgc gaagctgctg acgtcggcca
     2041 gctccaggct ctgaccctcc tgcagcgccg ggatctcctc gtcggcggag cgggcgtagg
     2101 tgtagatgcc ggcgtagccg gggtcgacta cgaccgagcc gcgcacgaag taggtctccg
     2161 agccggcctc gatgtcggcg cgggtggact cggtgatcat cggcgggctg aaggtggcga
     2221 ggaagcggcg cacgaccagt tcgtagaccc gcttcttgga gccgtcgagg gcgcccgggt
     2281 ggatcgcctg ggtcgggtag atcggcgggt ggtcggtggt ctcttttttg ccccgggtcg
     2341 gggtcagctc gccgtcgagc aggccggaag cggcggagaa ctctttgacc ctgaccagcg
     2401 aggagatcag ctcgcggacc ggcagcgagg acgggtagac cgtgttgtcg gtgcgcgggt
     2461 aggagatgaa cccgtccatg tagaggtcct cggcgatccg catcgcgctc gccggggtga
     2521 tcccgagccg gctggaggcg tcggtgctga aggcggtggt gttgtagggg gtcggcggtt
     2581 tgcgcgtgtt tttgcgcgcg gtcaccgatt tgacgacgcc gggacccttg gtccccgcga
     2641 acgctttgtc ggcttccgcc ttctcccaga atttgtcgac cgtgtggtgg gtctcgaaac
     2701 tgccgtcggg gtgctcgaac ttggcgaaca gctcccagaa cggtttcggc acgtgggcgc
     2761 ggcgctccat ctcgcgctcg acgatcaggc ccagggtcgg gctctgcacg cggccgaccg
     2821 agaggaagtt ggagccgaag cggcggctgg ccagcgagac cgcgcgggtc agggtcgcgc
     2881 cccagagcag gtcgatgtcc tggcgggcgg cgccggcgtt ggcgagcggg taggagagcg
     2941 tgtcgatctc gccgaaggcg cgctcgatct cttctttggt cagcgccgag tagcgggcgc
     3001 gctgcacgac cagcgtcccg gcctcggttt cctcgcgggt gccgagctcg gggttggcgt
     3061 cgagcatctc ctcgagcgcc tcgaggccga tcagctcgcc ctcgcggtcg aagtcggtgg
     3121 cgatcaccag ctcgtccgcg cctttggcca gtttcctgat cgctttgacg acgtttttgt
     3181 cggtcggttc cttgatcagc ttcgcgtcga tcaggtcgtg caggtcggtc ttctgccagt
     3241 tcgagtagcc ctcggggaag gcgggaccga gcacgtggcc cttgaggccg acggtcatgt
     3301 ggtcgccgtc gctgtcgctc cagctgtaga acgggacttt gaaggttttg tcctccttcg
     3361 ggccacccgt gggggctccg aggatttcgg cgatcttctt ggcgctgctg ttcttctcgg
     3421 tgacgatcag tctcatgggg cggcgagggt agcaggggcg cgcggccgtc tttctgcggc
     3481 gccgttcaca cgatccccag cctgcaccca gccgatcccc ataggatcgg ccccctgatg
     3541 aagaccttgg taaccggcgg cgcggggttt atcggctcga atctggtcga cggattgctc
     3601 gcgcgcggag acgaggtgac ggtcgtcgac gacctctcca ccggccgcct ggtcaacctc
     3661 gagggcgcac tggaggcggg cgcggagctg gtcgagctcg acatccgcga cgccgccggg
     3721 ctgaccgcgc tggtggccga gcgccggccc gaggcgatct tccacctggc cgcccagatc
     3781 gatgtgcgca agtcgctgga ggacccggcc ttcgacgccg cggtcaacgt cggcggcacc
     3841 gccaacgtgc tcgaggcggc gcggggggcg gcgaccgagc gggtcgtctt cgtctccacc
     3901 gggggcgcga tctacggcga gggcgagggt caggatctgc cgctcgacga gtcgacggcg
     3961 atcgcgccga tgtccgcgta cggccagagc aagtacgcgg ccgagggcta cctggccctc
     4021 tacgagcgcc tctacggact ctccactatc gccctgcgcc tcggcaacat ctatggcccg
     4081 cgccaggacc cgctcggcga ggccggcgtg gtggcgatct tctgcggcct ggtcagcagc
     4141 ggcggccgac cgaccgtcta cggcgacggc acccagaccc gcgactacat atatgtgggc
     4201 gacgtggtcg acgccgccct cgccgccgcc gactcacgcg ccggcggcgc gatcaacatc
     4261 ggcaccggcc gcgaggcgag cgtgctcgac ctggtcgaga tcctcggcca gctcggcggc
     4321 gccgccgact tcgagcccga gttcgccccg ccccgcgccg gcgaggtcca gcgcatctcc
     4381 cttgacgccg ccctcgccga acgcgagctc ggctggtccc cgcagaccag cctcgaggac
     4441 gggctgcggc tcactctcga cgcgatctga cgggcgtacc tcgggaaggc atgcggggag
     4501 gggtcgggtg actccctgca cttaactttt tcgtgggggc gggatagagc cgtccgagct
     4561 tgggggcg
//
LOCUS       DASRIG010000421         8125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_436082, whole genome shotgun sequence.
ACCESSION   DASRIG010000421 DASRIG010000000
VERSION     DASRIG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436082"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VFB52_11240"
     CDS             complement(<1..1264)
                     /locus_tag="VFB52_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZO06952.1"
                     /translation="MTLPPTPGPLPGPKSAAALQRISELVYPGSAEHVAPFVVASKSG
                     YLVEDLDGNTFVDLISASASVPLGAAREDLIEPLVETLRRTGNEDTHGIAHELVAPLA
                     ERLLAITPASLTRVDLSLNGTESVETAIKLMRRATGRPIVIGFLGQYHGESAATAALG
                     AEENEIARGIRPLTPGFVHAPYPNPYRSPLAPPRPGGSGDATVDYIRDHLLFHAVDPN
                     EVAGVLVEPILGSGGCVAPPESFWPALQELCDEHGWVLAVDEVKTGFGRSGEMFAVER
                     WGVEPELMCLGKAMGGGVAPIGAVLGSERVMGGYDDVPTGSTWSWLPWACAAALATLD
                     AYEREPVLENVRELERVGGERLAAIAEKFEQIGETRATGGFQAIEFVRDRASKERDPE
                     LQHLVARRAVERGVLMDSSSTSLNVQPSL"
     gene            1401..2852
                     /locus_tag="VFB52_11245"
     CDS             1401..2852
                     /locus_tag="VFB52_11245"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HZO06953.1"
                     /translation="MARTRQPIEPAEPTPESGFTRRTAIRDAAKLVVGAGAAAHVMAA
                     TGANNAVAATVREAARQVNRPGYGPLVQKTGEMSIPAGFTAIGFGEAYSKMDDGFLTP
                     PCHDGTGAFDMGNGIVRLVRNHEGYGQGKARGGNFRAYDRVSRGGVSTCVFDTKTGKV
                     LASHLVLNGTDTNCNGGVTPRGSWLTCEETTLGPRQGYEKKHGYVFEVPAGAKEVIEP
                     VPIKAMGRFEHEAAVTDPRSGIVYLTEDNGDPADGFYRYLPDNPNKLHRGGKLQMLAV
                     QGRSRYDTTSGQKVGKKLRCEWVDIDNPDPDDADRHPDAVFQQGRAKGAAQFMALEGA
                     KWKAGSCYFVASEGGDEELGQIWRYTPKGMKSGILTLLYESKSDGKLEEPDAIAVSPR
                     GGIVVCEDGDGDDVTGDNFIRVLSPAGKLETVARNDTPMDLVQWEEGDEGTFGRSEWS
                     GAVYSPDGKWLFVHIMYPGKTFAITGPWEQGWL"
     gene            2940..4025
                     /locus_tag="VFB52_11250"
     CDS             2940..4025
                     /locus_tag="VFB52_11250"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HZO06954.1"
                     /translation="MRDPLTEARDLAGWEPSLGVVSVYLGFDPGDRGGAWRTDLRNGL
                     DQLLERAKEADHERRVALRATAQRLLSRFEDGELRPPPHGEAGFVEVAESKGEERWWP
                     TSLAPAAGDCVQLGGRAAVAPLVDLRCRERECGVVLLSSERVRLLRLGGGELSELEEW
                     ELSITSLDWRERKASSGPNPTHGVSSSGHDQFHERLEHNRRRFLAECAGLVGARLHEH
                     DLDHALAFGLAHEQEEFASGFHAPGIVLEQGARADLISTPTGQLADHVLGAAEEAARS
                     RDRALVERVLEAARGGGRGAAGRQDVGEALAEGRVEQLVLDAAIGAPAEDLVRGALGG
                     HAKVALVRGETAEPLASAEGAAALLRY"
     gene            complement(4022..4849)
                     /locus_tag="VFB52_11255"
     CDS             complement(4022..4849)
                     /locus_tag="VFB52_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO06955.1"
                     /translation="MSNPQTDDTELKTRHRAMWASGDYPAMVETFLEPLGPRLVEATG
                     IGPGDRVLDVAAGTGNASIPAAATGAAVTASDLTPELFEAGRARAAAAGVELEWAGAD
                     AENLPFADDSFDVVISAIGVMFAPRHQDAADELVRVCRPGGRIALICWTPEGMIGDLF
                     RAMGPFAPTPPPGAQPPPLWGGEDHLRELFGERVELTQLERDKLEVTAFASADEFAEH
                     FKTLYGPTIATQANAEREGKSEELEAALDRVFAESAEERDGGTRFEMEYLLAVGTVR"
     gene            complement(4959..6062)
                     /locus_tag="VFB52_11260"
     CDS             complement(4959..6062)
                     /locus_tag="VFB52_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06956.1"
                     /translation="MPTADELLGPEVVARLTECLRGADGRRRWVSVEASTAGFAEQGL
                     SERVRAVQAALLEDLPPSYAASARIVRRALRGEDFDGWMIWPVGEAVAARAVASEHDD
                     DFEDGLALLAELTPRLSSEFAIRTFLAADLDRSLRTVRTWTEHPDEHVRRLASEGTRP
                     RLPWARRVRELIERPGVTIPILDALHDDPSEYVRRSAANHLNDVSRLDPALAVATARG
                     WLREGDAESARTVKRALRTLIKGADSDALALFGFTAPRDLVVEGILLEPEVVRVGEAL
                     GFAATITNAGTTACDLAIDYVVGHRKANGELSPKVFKLTTVRLEPGERAELSRRHSFK
                     PITTRRYYPGPHTIELQVNGARFGLAEFELLAP"
     gene            complement(6073..7146)
                     /locus_tag="VFB52_11265"
     CDS             complement(6073..7146)
                     /locus_tag="VFB52_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HZO06957.1"
                     /translation="MTDFLVKRGELGEVRIAESATPALGAGEALLRVDSFGMTANNVT
                     YAVFGEAMNYWDFFPAADGWGRVPVWGFAEVERSEVEGVEPGTRVFGYLPPSSHLLVT
                     PVALDGAGFVDGSSHKAALPSAYHRYLATEADPFYREGTEEVQMLLRPLFFTSFLIDD
                     QLADDSLTAKGPIVISSASSKTALAAAFLLSWRPEVRLVGLTSARNREFVEGLGIYGE
                     VVSYDEVGGMARGPATFVDCAGDAAVRESVHNHFGEELVASFTVGMTHWDRMGAGSGA
                     ELPGPDPQFFFAPDRVTKRSKDWGREILEARVAEAWHPFCDWTAGWIEPIRGKGFEAV
                     RDAYLQVLAGGVPPKRAHVLALP"
     gene            7203..8081
                     /locus_tag="VFB52_11270"
     CDS             7203..8081
                     /locus_tag="VFB52_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO06958.1"
                     /translation="MTTESEHPSEYEFVGYEQDGPVTTITFDRPERMNAIGPQMSAEL
                     IDAWTRFRADDDALVAVYTGRGSEAFCAGGDLKDAVDGKPVVPLDADERAAHVRGERP
                     GVLGPSRWTDLYKPTIAAINGVAYAGGLEWACWGDVAIADEHATFGVTCRRWNIGLAD
                     GGTQRLPRIVGFRRAMELIVTGRVIDAEEALAIGLVNEVVPSGEALPRAQELAQRVAA
                     LPQPALRTDKEAAVRGFGRDLAEGLRIEAECFDRSIFDPATAEGLRRFNERDHPDRRH
                     ADAPLTAGISRPRERS"
BASE COUNT         1153 a         2970 c         2901 g         1101 t
ORIGIN      
        1 gcagcgaggg ctggacgttg agcgaggtcg agctcgagtc catcagcacg ccgcgctcga
       61 ccgcccgccg cgcgaccagg tgctgcagct cggggtcgcg ctccttgctc gcccggtcgc
      121 ggacgaactc gattgcctgg aagccgccgg tcgcccgcgt ctcgccgatc tgctcgaact
      181 tctctgcgat cgccgccagg cgctcgccgc cgacccgctc cagctcgcgc acgttctcca
      241 gcaccggttc gcgctcgtag gcgtcgagcg tcgccagtgc cgccgcgcag gcccagggca
      301 gccaggacca ggtcgagccg gtcgggacgt cgtcgtagcc gcccatcacc cgctcgctgc
      361 cgagcacggc gccgatcggg gcgacgccgc cgcccatcgc cttgcccagg cacatcagct
      421 ccggttcgac cccccagcgc tcgaccgcga acatctcgcc ggagcggccg aagccggtct
      481 tcacctcgtc gaccgcaagc acccagccgt gctcgtcgca gagctcctgc agcgccggcc
      541 agaagctctc cggcggggcg acgcagccgc ccgagccgag gatcggctcg accagcacgc
      601 cggcgacctc gttcgggtcg accgcgtgga agagcaggtg gtcgcggatg tagtcgacgg
      661 tggcgtcgcc gctgccgccg ggccgcggcg gggccagcgg cgagcggtag gggttggggt
      721 agggggcgtg gacgaagccg ggggtcagcg gcctgatccc gcgggcgatc tcgttctcct
      781 cggccccgag cgccgcggtc gcggcgctct cgccgtggta ctggccgagg aatccgatca
      841 cgatcggccg gccggtcgcg cgccgcatca gcttgatcgc ggtctcgacc gactcggtcc
      901 cgttgaggct gaggtcgacc cgggtcagcg aggccggggt gatcgccagc agccgctcgg
      961 ccagtggcgc gaccagctcg tgggcgatcc cgtgggtgtc ctcgttgccg gtccgccgca
     1021 gggtctcgac cagcggctcg atcaggtcct cacgcgcggc gccgagcggg accgaggcgg
     1081 aggccgagat caggtcgacg aaggtgttgc cgtcgagatc ctcgaccagg tagcccgact
     1141 tcgaagcgac gacaaacggc gccacgtgct cggcgctgcc gggataaacc agttcggaaa
     1201 tccgctgcaa agcggcagcg ctcttcgggc cgggcagtgg gccgggggtg ggagggagcg
     1261 tcatcggctc tgcgccgggg tcttgacgat gtccatgacc cgtgagacta tcgatccacc
     1321 acaattgttc aacaattcat ctgttacgta attcctctaa gatccgccaa cccgacaccc
     1381 cccgcgccca ggagcagcca atggccagaa cccgtcagcc catcgagccc gccgagccca
     1441 cgccggagag cggcttcacc cgccgcaccg cgatccgcga cgccgccaag ctggtcgtcg
     1501 gcgccggtgc cgccgcccac gtgatggccg cgaccggggc caacaacgcc gtcgccgcga
     1561 ccgtgcgcga ggccgcccgc caggtcaacc gtcccggcta cggcccgctg gtccagaaga
     1621 ccggcgagat gtcgatcccc gccggcttca ccgcgatcgg cttcggcgag gcctactcga
     1681 agatggacga cggcttcctt accccgccct gccacgacgg caccggcgcc ttcgacatgg
     1741 gcaacgggat cgtgcgcctg gtccgcaacc acgagggcta cggccagggc aaggcgcgcg
     1801 gcggcaactt ccgcgcctac gaccgggtct cgcgcggcgg cgtctccacc tgcgtcttcg
     1861 acaccaagac cggcaaggtc ctggccagcc acctggtcct caacggcacc gacaccaact
     1921 gcaacggcgg cgtcaccccg cgcggcagct ggctgacctg cgaggagacc acgctcggcc
     1981 cgcgccaggg ctacgagaag aagcacggct acgtgttcga ggtcccggcc ggggccaagg
     2041 aagtgatcga gccggtgccg atcaaagcga tgggccgctt cgaacacgag gccgcggtca
     2101 ccgacccgcg cagcgggatc gtctacctga ccgaggacaa cggcgacccc gccgacggct
     2161 tctaccgcta cctgccggac aacccgaaca agctgcaccg cggcggcaag ctgcagatgc
     2221 tcgcggtcca gggccgctcc cgttacgaca cgaccagcgg gcagaaggtt ggcaagaagc
     2281 tgcgctgcga gtgggtcgac atcgacaacc ccgatcccga cgacgccgac cgtcatcccg
     2341 acgcggtctt ccagcagggc cgggcgaagg gcgccgcgca gttcatggcg ctggagggtg
     2401 ccaagtggaa ggccggcagt tgttacttcg tcgcctccga aggcggcgac gaagaactcg
     2461 gccagatctg gcgctacacc ccgaagggga tgaagtccgg gatcctcacc ctgctctacg
     2521 agtcaaagag cgatggcaag cttgaagaac ccgacgcgat cgcggtcagc ccccgcggcg
     2581 gcattgtcgt ctgcgaggac ggcgatggcg acgacgtgac cggcgacaac ttcatccgcg
     2641 tcctctctcc cgccggcaag ctcgaaaccg tcgcccgcaa cgacaccccg atggacctgg
     2701 tccagtggga ggaaggcgac gagggcacct tcggccgcag cgagtggtcg ggcgccgtct
     2761 acagccccga cggcaagtgg ctgttcgtcc acatcatgta cccgggcaag accttcgcga
     2821 tcaccgggcc gtgggagcag ggctggctgt gatcccgagc atcgtccccg ctcgggacct
     2881 gatcaaaggc gaggactaat tcgcgcattt ctccccgccg gctgggtacg gtggggagga
     2941 tgcgagatcc gctgaccgag gcgcgcgacc tggccgggtg ggaaccgtcg ctcggtgtcg
     3001 tctccgttta cctcggcttc gaccccggcg atcgcggcgg tgcctggcgg accgacctgc
     3061 gcaacgggct cgaccagctg ctggagcggg ccaaggaggc cgatcacgag cgccgcgtcg
     3121 ccctgcgggc gaccgcgcag cggctgctgt cgcggttcga agacggcgag ctgcgcccgc
     3181 cgccgcacgg cgaagcgggc ttcgtcgagg tcgccgagtc aaagggcgag gagcgctggt
     3241 ggccgacctc gctggcgccc gcggccggcg actgcgtgca gctcggcggg cgcgccgcgg
     3301 tagcgccgct ggtcgacctg cgctgccgcg aacgcgagtg cggggtggtc ctgctctcct
     3361 ccgagcgggt gcgcctgctg cggctcggcg gcggcgagct cagcgagctg gaggagtggg
     3421 agctgagcat caccagcctc gattggcgtg agcgcaaggc ctcctcgggc ccgaacccga
     3481 cccacggggt cagctcctcc gggcacgacc agttccacga gcggctcgag cacaaccggc
     3541 gccgcttcct cgccgagtgc gccggcctgg tcggggctcg cctgcacgag cacgacctcg
     3601 accacgcgct cgccttcggc ctcgcccacg agcaggagga gttcgcctcc ggcttccacg
     3661 cgcccgggat cgtgctcgag cagggcgccc gggccgacct gatctcgacc ccgaccggcc
     3721 agctcgccga ccacgtgctc ggcgccgccg aggaggcggc ccgctcccgc gatcgggcgc
     3781 tggtcgagcg cgtgctcgaa gccgcccgcg gcggcggccg cggcgccgcc gggcgccagg
     3841 acgtcggcga ggcgctggcc gaaggccggg tcgagcagct ggtgctcgac gcggcgatcg
     3901 gcgcgccggc cgaggatctg gtgcgcgggg cgctcggcgg ccacgccaag gtggcgctgg
     3961 tacgcggtga gaccgccgag ccgctggcaa gcgccgaggg cgccgctgcg ctcctgcgct
     4021 actagcgcac ggtcccgacc gcgagcaggt actccatctc gaagcgggtg ccgccgtcgc
     4081 gctcctcggc gctctcggcg aagacccggt cgagtgccgc ctcgagctcc tcgctcttcc
     4141 cctcgcgctc ggcgttcgcc tgggtcgcga tcgtcggccc gtagagcgtc ttgaagtgct
     4201 cggcgaactc gtccgcgctc gcgaacgcgg tcacttcgag cttgtcgcgc tccagctggg
     4261 tcagctcgac ccgctcgccg aacagctcgc gcaggtggtc ctcgccgccc cacagcggtg
     4321 gcggctgggc gccgggcggg ggagtgggcg cgaacggtcc catcgcgcgg aagaggtcgc
     4381 cgatcatccc ctccggggtc cagcagatca gcgcgatccg gccgcccggc cggcagaccc
     4441 ggaccagctc gtcggcggcg tcctggtggc gcggcgcgaa catcaccccg atcgccgaga
     4501 tcacgacgtc gaaggagtcg tcggcgaagg gcaggttctc ggcgtcggcg cccgcccact
     4561 ccagctcgac cccggcggcg gcggcgcggg cgcgccccgc ctcgaacagc tccggggtga
     4621 ggtcgctggc ggtgaccgcg gcgccggtcg cggcggccgg gatcgaggcg ttgccggtgc
     4681 cggcggcgac gtcgagcacc cggtcgcccg gcccgattcc cgtcgcctcg accaggcgcg
     4741 gccccagcgg ttcgaggaag gtctcgacca tcgccgggta gtcgcccgag gcccacatcg
     4801 cccggtggcg ggtcttcagc tcggtgtcgt cggtctgcgg gttgctcatg cgctctctcc
     4861 cttgtagacc cgaccggttg gatcggggtt cccgttcttt ctccccgatc ggcgcccccg
     4921 ggtctgtgcc gtttctacac ggcgctgtgt gacggctctt acggtgcgag cagctcgaac
     4981 tcggcaaggc cgaagcgggc gccgttgacc tgcagctcga tcgtgtgcgg gccggggtag
     5041 tagcggcggg tggtgatcgg cttgaacgag tggcggcggg agagctcggc gcgctcgcca
     5101 ggctcgagcc gcacggtggt cagcttgaac accttcgggc tgagctcgcc gttggctttg
     5161 cggtggccga ccacgtagtc gatcgccagg tcgcaggcgg tggttccggc gttggtgatc
     5221 gtcgccgcga agccgagcgc ctcgccgacc cggaccacct cgggttcgag caggatcccc
     5281 tcgaccacga ggtcgcgcgg cgcggtgaag ccgaacagcg ccagggcgtc gctgtcggcg
     5341 cccttaatca gcgtgcgcag cgcccgcttg accgtgcggg ccgactcggc gtcgccctcg
     5401 cgcagccagc cgcgcgcggt cgcgacggcc agcgccgggt cgagccggct gacgtcgttg
     5461 aggtggttgg cggcggagcg gcgcacgtac tcggaggggt cgtcgtgcag ggcgtcgagg
     5521 atcgggatcg tgacgccggg tcgctcgatc agctcgcgga cccgccgcgc ccagggcagc
     5581 cgcggccgcg tcccctcgct ggccagccgg cgcacgtgct cgtcggggtg ctcggtccag
     5641 gtccgcacgg tgcgcagcga gcggtcgagg tcggcggcga ggaaggtgcg gatcgcgaac
     5701 tcgctgctca gccgcggggt cagctcggcg agcagcgcca ggccatcctc gaagtcgtcg
     5761 tcgtgctcgg atgcgaccgc ccgcgccgcg accgcctcgc cgaccggcca gatcatccag
     5821 ccgtcgaagt cctcgccgcg cagcgcccgc cgcacgatcc gcgcgctcgc cgcgtagctc
     5881 ggcggcagat cctcgagcag cgccgcctgc accgcgcgca cccgttcgct cagcccctgc
     5941 tcggcgaagc cggcggtgct cgcctcgacg ctcacccagc gccgtcgccc gtcggcgccg
     6001 cgcaggcact cggtcaggcg cgcgacaacc tcagggccca gcagttcgtc ggcggtcggc
     6061 atcgccgcca ggctatggca gggccagcac gtgggcgcgc ttcggcggca cgccgccggc
     6121 gagcacctgc aggtaggcgt cgcgcaccgc ctcgaagccc ttgccgcgga tcggctcgat
     6181 ccagcctgcg gtccagtcgc agaacgggtg ccaggcctcg gcgacgcgcg cctcgaggat
     6241 ctcccgtccc cagtccttgc tgcgcttggt cacccggtcg ggggcgaaga agaactgcgg
     6301 gtccggcccg ggcagctcgg cgccggagcc ggcgcccatc cggtcccagt gggtcatccc
     6361 gacggtgaag ctggcgacga gctcctcgcc gaagtggttg tggacggact cgcgcacggc
     6421 ggcgtcgccg gcgcagtcga cgaaggtcgc cgggccgcgc gccatgcccc cgacctcgtc
     6481 gtagctgacg acctcgccgt agatccccag cccctcgacg aactcgcggt tgcgcgcgga
     6541 ggtgaggccg accagccgca cctccgggcg ccaggagagc aggaaggccg ccgccagcgc
     6601 ggtcttgctc gaggcgctgg agatcacgat cggccctttg gcggtgaggc tgtcgtcggc
     6661 gagctggtcg tcgatcagga acgaggtgaa gaacagcggg cgcagcagca tctgcacctc
     6721 ctcggttccc tcgcggtaga acgggtcggc ctcggtggcg aggtagcggt ggtaggcgga
     6781 gggcagcgcc gctttgtggg aagagccgtc gacgaagccg gcgccgtcga gcgcgaccgg
     6841 ggtgaccagc aggtgcgagg agggcggcag gtagccgaac acccgcgtgc ctggctcgac
     6901 cccctcgacc tcgctgcgct cgacctcggc gaatccccag accggcaccc gtccccagcc
     6961 gtccgcggcg gggaagaagt cccagtagtt catcgcctcg ccgaagaccg cgtaggtgac
     7021 gttgttcgcg gtcatcccga agctgtcgac ccgcagcagc gcctcgcccg cgccgagcgc
     7081 cggcgtcgcc gactcggcga tccgaacctc gcccagctca ccgcgtttca ccagaaagtc
     7141 ggtcatctcc ctcgccccgg ccaggctatc cgctcgtgct cacagtgaca aactcccgga
     7201 ccatgactac cgagagcgag caccccagcg agtacgagtt cgtcggctac gagcaggacg
     7261 ggccggtgac cacgatcacc ttcgaccgac cggagcggat gaacgcgatc ggcccgcaga
     7321 tgagcgccga gctgatcgac gcctggaccc gcttccgcgc ggacgacgac gccctcgtcg
     7381 ctgtctacac cggtcgcggc agcgaggcct tctgcgccgg cggcgacctc aaggacgcgg
     7441 tcgacggcaa gccggtggtg ccgctcgacg cggacgagcg agctgcgcat gtgcggggcg
     7501 agcgccccgg cgtgctcggc ccctcgcgct ggacggacct ctacaagccg acgatcgccg
     7561 cgatcaacgg ggtcgcctac gccggcggcc tcgagtgggc ctgctggggc gacgtcgcga
     7621 tcgccgacga gcacgcgacc ttcggcgtca cctgtcggcg ctggaacatc ggcctcgccg
     7681 acggcggcac ccagcggctg ccgcggatcg tcggcttccg ccgggcgatg gagctgatcg
     7741 tcaccggccg cgtgatcgac gccgaggagg cgctggcgat cggcctcgtc aacgaggtcg
     7801 tgccgagcgg cgaggcgctg ccgcgtgccc aggagctggc ccaacgggtc gcggcgctgc
     7861 cgcagcccgc gctccgcacc gacaaggagg cggcggtgcg cggcttcggc cgggacctcg
     7921 ccgaggggct gcggatcgag gccgagtgct tcgaccgctc gatcttcgac ccggcgaccg
     7981 ccgagggcct gcgccgcttc aacgagcgcg accacccaga tcgccgccac gccgacgcgc
     8041 cgctgaccgc cggcatctcc cgcccgcgcg agcgctccta actcttcaga agcgcgaatc
     8101 caccttgtcg gtgcgccatc tccgc
//
LOCUS       DASRIG010000422         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2270529, whole genome shotgun sequence.
ACCESSION   DASRIG010000422 DASRIG010000000
VERSION     DASRIG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270529"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFB52_11275"
     CDS             <1..413
                     /locus_tag="VFB52_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06959.1"
                     /translation="RVWHGVLAIVAALAVGAAGTAIAGGGQAKDLTTAGTPPGGIFRD
                     SGKYHYGFKAKGTPVGSSQENVRLKCPRGTRAIAGGGGGFSLDPTGQNVNYQRPYDSR
                     DRGRVPEDGWIVFVNTVEGEGEGIGVEVICIDKR"
     gene            complement(410..829)
                     /locus_tag="VFB52_11280"
     CDS             complement(410..829)
                     /locus_tag="VFB52_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZO06960.1"
                     /translation="MEAATARKIRVVVAKPGLDGHDRGAKIIARALRDAGMEVIYTGL
                     HQTPEQIAETVIQEDADAVGLSILSGAHMTLVPRVVKLLREQGVDDVLITVGGTIPAE
                     DAPELKKLGIAEVFTPGASTDEIVEFIRGAVAERMAS"
     gene            complement(864..1640)
                     /locus_tag="VFB52_11285"
     CDS             complement(864..1640)
                     /locus_tag="VFB52_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZO06961.1"
                     /translation="MSRAAAFFDLDKTLMAGSSGMQFARVATQQGIVSRRQLAHWGFE
                     HLRYRLRGTTDERTADVLKVARELIAGVPAKAIARMEPEVMAEILPRVYPQMLAEMHA
                     HQDAGRPTFIVSAAGNDIVESLARVLGMDGGIGTRYEIDPEGNFTGRLDGPFVYGPGK
                     VEAMEAFAAEHDIDLAESYAYSDSLSDLPMLRAVGNPVAVNPDPPLAELAREEGWQTL
                     RFERLGRRLIALGATVIASAAAFGAARHRQRSRSTGRSRK"
     gene            complement(1684..2859)
                     /locus_tag="VFB52_11290"
     CDS             complement(1684..2859)
                     /locus_tag="VFB52_11290"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZO06962.1"
                     /translation="MPSSVILGTARTPIGKMGGALSTLDATDLGGTAIEAALSRADVA
                     PEQVQQVIYGQVLQAGQGQIPSRQAQIKGGIPKEVPSETINKVCASGMRTIALADQAI
                     RVGDTEVAVTGGMESMSKAPYLLPNARFGFRMGDVQALDAMTHDGLTNAFTGKQMINE
                     ASEVSNELEITRVDMDRFAERSQHLASEAIESGRIADEIVEVTVKGRKGDTVVETDEG
                     PRPETTLEALSKLRAVGGEGATHTAGNAPGVNDGAGAIVVASEEWAQKEGKTPLAKVL
                     SYGTVADDFAYLARTPGNAALVALEKIGKKPEDVDLWEINEAFASVAIGSMRILGIGE
                     ENLNVNGGAIAFGHPIGASGSRIVGSLVHELRRRGGGLGVAAICSGGGQGDAIVVEV"
     gene            2915..4348
                     /locus_tag="VFB52_11295"
     CDS             2915..4348
                     /locus_tag="VFB52_11295"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZO06963.1"
                     /translation="MKVDVRDIQHSEADADVLAVGLYEDGSLPTEIADAAGAEAAKGE
                     FKKLSPAFAGGARIVVAGLGKREDLDAEKLRVVAALVAKQAAGLEAGSLAWALPESDD
                     AAAAAEAIVTGTILGSYSFDRFKKAPDEEPARLDALSLLASAEVAEAAETARVYAEAA
                     NRARDLQSTPANFEKPEDLAARAEQIAAGSEALSAEILGPAELEEKGMGGLLAVSQGG
                     PVEPRMIVLRYAGGGSGPTLGLVGKGVTFDTGGISLKPGAGMHEMKYDMSGAAAVLEA
                     VAAIAELGLAIDLVAVVPSTENMPSGTAVKPGDVITHYNGTTVEVNNTDAEGRLILAD
                     ALAYSVELGAERIVDLATLTGAVVIALGSTYAAVISNDDELAAAVAEAGERSGELTWR
                     LPLHPEFKKLMKGTVADLSNLASQRKAGTITAASFLEEFVDKTPWAHIDIAGTAWDVG
                     REYTGKDASGYGVRLLVELARGLATPS"
     gene            complement(4383..4955)
                     /locus_tag="VFB52_11300"
     CDS             complement(4383..4955)
                     /locus_tag="VFB52_11300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZO06964.1"
                     /translation="MSSDGIVFAPDATAADVAEAFAASSPETTRRRELVWQDPVATAA
                     EGATLTGMEYMEAIVSGKMPPPPIAVTMLLRPIELEEGRVIFEGEPGEEHYNPIGVVH
                     GGYAATLLDSALGCAVHTTLPAGVGYTSLGIEAKYVRPITRDTGRVLCEAKVLYRGRK
                     QATAEATLTAAASGKLLASGTATCMILGAA"
     gene            complement(4952..5107)
                     /locus_tag="VFB52_11305"
     CDS             complement(4952..5107)
                     /locus_tag="VFB52_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06965.1"
                     /translation="MRFVHLIPRNDQRRRGLRIEISQAEITADLHYPAGSRQAREATV
                     ARQAGGR"
     gene            5218..5700
                     /locus_tag="VFB52_11310"
     CDS             5218..5700
                     /locus_tag="VFB52_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO06966.1"
                     /translation="MLGHAYPGQICSVAGALEVIGERWSLLIVRDVMNGHRRFGELQR
                     SLGVARNVLSARLQRLVEEGILERRAYQESPPRHEYFLTEKGLDLWPALVALQGWGDR
                     HSTGPEGPPMLIVHKQCGGTVSDRGVCESCGETLHARDARAVPGPGLATALAELEASG
                     "
BASE COUNT          844 a         2021 c         2102 g          860 t
ORIGIN      
        1 tgagggtctg gcacggggtg ctcgcgatcg tcgcggcgct cgcggtcggg gccgccggca
       61 cggcgatcgc gggcggcggc caggccaagg acctgaccac ggccgggacc ccccctggcg
      121 ggatcttccg ggactccggg aagtaccact acgggttcaa ggcgaagggg accccggtcg
      181 gcagtagcca ggagaatgtg aggctcaagt gcccgcgcgg cacgcgggcg atcgccggcg
      241 gcggaggggg attcagcctc gacccgaccg ggcagaatgt caactaccag cgcccgtacg
      301 acagccgcga ccgcgggcgc gttcccgagg acggctggat cgtattcgtg aacaccgtcg
      361 agggcgaggg cgagggcatc ggcgtggagg tgatctgcat cgacaagcgc taagaggcca
      421 tccgctcggc gaccgcaccg cggatgaact cgacgatctc gtcggtggag gcgccggggg
      481 tgaacacctc ggcgatgccg agcttcttca gctccggcgc gtcctcggcc ggaatcgtcc
      541 caccgaccgt gatcagcacg tcgtccacgc cctgttcgcg cagcagcttg acgaccctcg
      601 gcaccagggt catgtgggcg ccggagagga tcgagaggcc gaccgcgtcg gcgtcctcct
      661 gaatcacggt ctcggcgatc tgctcggggg tctggtgcag gcccgtgtag atcacctcca
      721 tgccggcgtc gcggagggcg cgagcgatga tcttggcgcc acggtcgtga ccatcgaggc
      781 cgggcttggc gacgacgaca cggatcttcc tggcggttgc agcctccatc gcagcaagcg
      841 tattgaaaag ggaggggttc cgcctatttc cgcgagcgcc cggttgagcg gctgcgctgg
      901 cgatggcgcg cagcgccgaa ggcggctgcg ctggcgatca ccgtcgcccc gagcgcgatc
      961 agacggcggc cgaggcgttc gaagcgcagc gtctgccagc cctcctcacg ggccagctcg
     1021 gccagcgggg ggtcggggtt aaccgcgacc gggttgccga ccgcgcgcag catcggcagg
     1081 tccgagagcg agtcggagta ggcgtaggac tcagccaggt cgatgtcgtg ctcggccgcg
     1141 aacgcctcca tcgcctcgac cttgccgggt ccgtagacga aggggccatc gaggcggccg
     1201 gtgaagttgc cctctgggtc gatttcgtag cgggtgccga tcccgccgtc catgccgagc
     1261 acgcgggcga gcgattcgac gatgtcgttg ccggcggcgc tgacgatgaa ggtcggccgg
     1321 ccggcgtcct ggtgggcgtg catctcggcg agcatctgcg gatagacgcg cggcaggatc
     1381 tccgccatca cctccggctc catccgcgcg atcgccttcg ccggcacgcc ggcgatcagc
     1441 tcgcgcgcga ccttgagcac gtcggcggtg cgctcgtcgg tggtcccgcg cagccggtag
     1501 cgcaggtgct cgaaccccca gtgggcgagc tgccgccggc tgacgatccc ctgctgggtc
     1561 gcgacccgcg cgaactgcat ccccgaagaa cctgccatca gggtcttgtc caagtcgaag
     1621 aatgcggctg cgcgactcat cgctgcgtgg gggcgccgcg gaccggcgcc ccctctacct
     1681 cgatcagacc tcgacgacga tcgcgtcgcc ctggccgccg cccgagcaga tcgcggcgac
     1741 gccgaggcca ccgccgcggc ggcgcagctc gtgcacgagc gagccgacga tgcgcgagcc
     1801 ggaggcaccg atcgggtggc cgaaggcgat cgctccgccg ttcacgttga ggttctcctc
     1861 gccgatgccg aggattcgca tcgagccgat cgcgaccgaa gcgaaggcct cgttgatctc
     1921 ccagaggtcg acgtcctcgg gcttcttgcc gatcttctcc agcgccacca gagcggcgtt
     1981 gccgggggtg cgggcgaggt aggcgaagtc gtcggcgacg gtgccgtagg agagcacctt
     2041 ggccagcggc gtcttgcctt ccttctgcgc ccactcctcg gaggcgacga cgatcgcgcc
     2101 ggcgccgtcg ttgacgcccg gggcgttgcc agcggtgtgg gtcgcgccct cgccgccgac
     2161 cgccctcagc ttcgagagcg cctccagggt ggtctccggg cgcgggccct cgtcggtctc
     2221 gaccacggtg tcgcccttgc ggcccttgac cgtgacctcg acgatctcgt cggcgatccg
     2281 gccggactcg atcgcctcgg aggcgaggtg ctgggagcgc tcggcgaagc ggtccatgtc
     2341 gacgcgggtg atctcgagct cgttcgagac ctcgctcgcc tcgttgatca tctgcttgcc
     2401 ggtgaaggcg ttggtgaggc cgtcatgggt catcgcgtca agcgcctgca cgtcgcccat
     2461 ccggaagccg aagcgggcat tcgggagcag gtacggcgcc ttgctcatcg actccatgcc
     2521 gccggtcacg gcgacctcgg tgtcgccgac gcggatcgcc tgatcggcga gcgcgatcgt
     2581 gcgcatgccg gaggcgcaga ccttgttgat cgtctccgag gggacctctt tcgggatccc
     2641 gcccttgatc tgggcctggc gcgaggggat ctgaccctgg ccggcctgca ggacctggcc
     2701 gtagatgacc tgctggacct gctcgggggc gacgtcggcc cggctcagcg ccgcctcaat
     2761 cgcggtgccg cctaggtcgg tcgcgtcgag ggtcgagagg gcgcctccca tcttgccgat
     2821 cggggtgcgc gcggttccga ggatgactga gctgggcatt ccctgccttt cgttccggag
     2881 aattcgagtg gcgacaagca tagactcgcg cccgatgaag gtggacgtgc gtgacatcca
     2941 gcacagcgag gccgacgccg acgtgctcgc ggtcgggctc tacgaggacg gctcgctgcc
     3001 gacggagatc gccgacgcgg ctggggccga ggccgccaag ggcgagttca agaagctctc
     3061 ccccgccttc gccggcggcg cgcggatcgt cgtcgccggg ctcggcaagc gcgaggacct
     3121 cgatgccgag aagctccggg tagtcgccgc cctggtcgcc aagcaggcgg ccggactgga
     3181 ggccggctcg ctggcctggg cgctgccgga gagcgatgac gcagccgccg cggccgaggc
     3241 gatcgtcacc gggacgatcc tcggctccta ctctttcgac cgcttcaaga aggcgccgga
     3301 cgaggagccg gcgcggctcg acgcgctgtc cctgctggcc agcgccgagg tcgccgaggc
     3361 ggccgagacc gcgcgcgtct acgccgaggc cgcgaaccgc gcccgcgacc tgcagagcac
     3421 gcccgcgaac ttcgagaagc ccgaggacct cgccgcccgc gccgaacaga tcgccgccgg
     3481 ctccgaggcg ctcagcgccg agatcctcgg ccccgcagag ctcgaggaga agggcatggg
     3541 cggactgctc gcggtcagcc agggcgggcc ggtcgagccg cggatgatcg tcctgcgcta
     3601 cgccggcggc ggcagcgggc cgacgctcgg cctggtcggc aagggagtga ccttcgacac
     3661 cggcgggatc tcgctgaagc ctggcgccgg catgcacgag atgaagtacg acatgtcggg
     3721 cgcggcggcg gtgctggagg cggtcgccgc gatcgccgag ctgggactgg cgatcgacct
     3781 ggtcgcagtc gtgccctcga ccgagaacat gccgtcgggc accgcggtca agccgggcga
     3841 cgtgatcacc cactacaacg gcaccacggt cgaggtgaac aacaccgacg ccgagggccg
     3901 actgatcctc gccgacgcgc tcgcctactc ggtcgagctc ggcgccgagc ggatcgtcga
     3961 cctggcgacg ctgaccggcg cggtcgtgat cgccctcggc tcgacctacg ccgcggtgat
     4021 ctccaacgac gacgagctgg cggccgcggt tgccgaggcc ggcgagcgca gcggtgagct
     4081 gacctggcgc ctgcccctcc accccgagtt caagaagctg atgaagggca ccgtcgccga
     4141 cctctccaac ctcgcctcgc agcgcaaggc cggcacgatc accgccgcct ctttcctcga
     4201 ggagttcgtc gacaagaccc cttgggccca catcgacatc gccggcaccg cctgggacgt
     4261 cggccgcgag tacacgggga aggacgcgag cggctacggc gtgcggctgc tggtcgagct
     4321 cgcgcgcggc ctcgcgaccc cgtcctaacg cacgctatgc gtgcataagc gcgggttcac
     4381 gctcaggccg caccgaggat catgcaggtc gcggtcccgc tggcgagcag cttgccgctg
     4441 gccgccgcgg tcagggtcgc ctcggcggtc gcttgtttgc ggccccggta gaggaccttg
     4501 gcctcgcaga ggacgcggcc ggtgtcgcgg gtgatcgggc gcacgtactt ggcctcgatc
     4561 ccgagcgacg tgtagccgac gccggcgggc agggtcgtgt gcacggcaca gccgagcgcc
     4621 gagtcgagca gggtcgcggc gtagccgccg tggacgacgc cgatcgggtt gtagtgctcc
     4681 tcccccggtt cgccctcgaa gatcacccgc ccctcctcca gctcgatcgg gcgcagcagc
     4741 atcgtcaccg cgatcggcgg cggcggcatc ttgccggaga cgatcgcctc catgtactcc
     4801 atcccggtca gcgtcgcgcc ctcggccgcg gtcgcgaccg ggtcctgcca gaccagctca
     4861 cggcggcgcg tcgtctccgg gctggaggcg gcgaacgcct cggcaacgtc cgccgccgtc
     4921 gcgtcgggag cgaaaacgat cccgtcgctg ctcaccggcc gcccgcctgc cgggcgacgg
     4981 tcgcctcccg agcctggcgc gagcccgctg ggtagtgcag gtcggcggtg atctcagcct
     5041 ggctgatctc gatccgcagg cccctgcggc gctggtcgtt acggggtatg aggtggacga
     5101 atctcatggt cgcctccaat cggcgcactg attgttggtt gcatcaagaa actgacgtag
     5161 ggcggcagcc taaccgagat ggttgcttga tgcaaccaac tgtgcgaaga tcacaccatg
     5221 ctcggacacg cctaccccgg tcagatctgc tccgtggcgg gagcgttgga ggtgatcggc
     5281 gagcgctggt cgctgctgat cgtgcgcgac gtgatgaacg ggcaccgccg cttcggcgag
     5341 ctgcagcgct cgctcggggt cgcccgcaac gtgctctccg cccgcctgca gcggctggtc
     5401 gaggagggca tcctcgaacg ccgcgcctac caggaaagcc cgccccgcca cgagtacttc
     5461 ctgaccgaga agggcctcga cctctggccc gccctcgtcg cgctgcaggg ctggggcgac
     5521 cgccactcga ccggcccgga ggggccaccg atgctgatcg tccacaagca gtgcggcggc
     5581 accgtctccg accgcggagt ctgcgagagc tgcggcgaga cgctgcacgc ccgcgacgcc
     5641 cgcgcggtcc ccggccccgg cctggccacc gccctggccg agctcgaggc gtcgggctag
     5701 ccgcgcgccc aggtccaggc ggtaccgtgt tcccggaagg gatcgggaac tcgaaggagg
     5761 agcgggaatg gcgacggcgg cggagcttca ggcgcgggaa aaggtcttta tcggcggcga
     5821 gtgggtc
//
LOCUS       DASRIG010000423         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_967658, whole genome shotgun sequence.
ACCESSION   DASRIG010000423 DASRIG010000000
VERSION     DASRIG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967658"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1042)
                     /locus_tag="VFB52_11315"
     CDS             complement(128..1042)
                     /locus_tag="VFB52_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HZO06967.1"
                     /translation="MADQIKVAYWPGCVSRGFTPELHGSMALVADKLGIELVTLDRAN
                     CCGAGVIAEHNQELADTLNARTFALAQQTGLSMMNICSTCQGAQSECQQRLDADSAYR
                     AHVNEALSGQGLSYEKSVDGSWNNKNFLWLLVEDYGLDRLASLVKRPLTGLRVGPFYG
                     CYIVRPKERLGYHDYPDRDVYLEQVIKVLGGEVVEYDGARKCCGFPVITMNRETSLKQ
                     AGTHVGDAIDAGADCLVTPCPLCHLNLDLQQPAATNYVERDLGIPVLHLPQMVGLALG
                     LDPKELGMGKHVVSTKDVQRKLMAAAAA"
     gene            complement(1035..2219)
                     /locus_tag="VFB52_11320"
     CDS             complement(1035..2219)
                     /locus_tag="VFB52_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZO06968.1"
                     /translation="MADYTIKVRRFQPESGQGPYWEQFNVELEPSLSVLDALLQAKDR
                     EDGSLAVRCSCRAAICGSCGMKINGQSGLGCKTQIGEAQELADKKAFAVGGESAGNVV
                     VIEPMGNMPVIKDLVTDMESTHWAKIRRVTPWLLPHGAPPEREYVVEPESMLDITQPM
                     ACIQCGSCVSACLSMEADPEFIGPAALAKAYRFVGDPRDAETKERLHDLAEDPHGIYD
                     CTHCFSCVDACPKDVAPMDQIMRLRRRAGEEGIEDPNSGHSHEMAFVKIIEKKGTLDE
                     SLLLQESFAPGIKGKLKPTKRAIKEMLGSLPTAIRGIKTGKMRSLPKLIPGVHHKLPG
                     GAQDEVKAIYKHAEEHNEEFNLYIKGEEDVEPEPDEPTDATLAAEAAASAAESEEATN
                     NG"
     gene            complement(2219..>2940)
                     /locus_tag="VFB52_11325"
     CDS             complement(2219..>2940)
                     /locus_tag="VFB52_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZO06969.1"
                     /translation="AGVDITREPIHIKPGNHYTMGGVKTDINGRTDIPGLYAAGETAC
                     VSVHGGNRLGANSLLDTLIFGKRSGRDAASRAKRIEGTRPSKQVLEDEEAMIADINSR
                     EKGTGRRVSEIKLELGQTMDGKVAVFRDEAGIQEALEIVRRLKEEAKTAYIDDHGSVF
                     NQDLLGAIELGYMLDNAECTCIAALERKESRGAQYRTDYPERNDEEWLKHIDLVKTDG
                     DPEISYSEVTITQWQPEARTY"
BASE COUNT          405 a          948 c         1015 g          572 t
ORIGIN      
        1 gacgaacggg cctccggcgg gaatccttca ggcacagaac ttcacacagg gcggaaatgg
       61 ggtacgccca gcgagagccg accaaggaat ggtcggtttt tcgcgttagg agggcgctcc
      121 gaccccgcta agcggcggcc gccgccatca gcttgcgctg gacgtccttg gtggagacga
      181 cgtgcttgcc catgccgagc tccttcgggt cgagcccgag cgcgaggccc accatctgcg
      241 gcaggtggag gaccgggatc ccgaggtcgc gctccacgta gttcgtggca gccggctgct
      301 gcagatcgag attcaggtgg cagagcgggc agggggtgac gagacagtcg gccccggcgt
      361 cgatcgcgtc gccgacgtgg gtcccggcct gtttcagcga ggtctcgcgg ttcatcgtga
      421 tcaccgggaa gccgcagcat ttgcgcgccc cgtcgtactc gacgacctcg ccgcccagca
      481 ccttgatcac ctgctcgagg tagacgtcgc ggtccgggta gtcgtggtag ccgagccgct
      541 ccttcggccg cacgatgtag cagccgtaga aggggccgac ccgcagcccg gtcagcggcc
      601 gcttgaccag cgaggcgagc cgatcgaggc cgtagtcctc gaccagcagc cagaggaagt
      661 ttttgttgtt ccaggagccg tcgaccgatt tctcgtagga gaggccctgg ccggagagcg
      721 cctcgttgac gtgggcccgg taggccgagt cggcgtcgag ccgctgctgg cactctgact
      781 gcgcgccctg gcaggtcgag cagatgttca tcatcgagag cccggtctgc tgggccagcg
      841 cgaaggtgcg cgcgttcagc gtgtcggcga gctcctggtt gtgctcggcg atcacgccgg
      901 cgccgcagca gttggcgcgg tcgagcgtta ccagctcgat cccgagcttg tcggcgacca
      961 gcgccatcga gccgtgcagc tccggggtga agccgcgcga gacgcaaccg ggccagtagg
     1021 cgactttgat ctgatcagcc attgttcgtg gcctcctcgc tctcggcggc acttgccgcg
     1081 gcctcggccg ccagggtggc gtccgtcggc tcatcgggct cgggttcgac gtcctcctcg
     1141 cccttgatgt acaggttgaa ttcctcgttg tgctcctcgg cgtgcttgta gatcgccttg
     1201 acctcgtcct gtgcaccgcc cgggagcttg tggtggacgc cggggatcag cttcgggagc
     1261 gagcgcatct tgcccgtttt gatcccccgg atcgccgtcg gcagtgagcc gagcatttcc
     1321 ttgatcgccc gtttcgtcgg tttcagcttg cctttgatcc cgggggcgaa ggactcctgc
     1381 agcagcaggc tctcgtcgag cgtgcccttc ttctcgatga tcttgacgaa ggccatctcg
     1441 tgggagtgac cgctgttggg atcctcgatc ccttcttcgc cggcgcggcg gcgcaggcgc
     1501 atgatctggt ccatcggcgc cacgtccttc gggcaggcgt cgacgcagct gaagcagtgg
     1561 gtgcagtcgt agatcccgtg cgggtcctcg gcgaggtcgt gcaggcgctc tttggtctcg
     1621 gcgtcgcgcg ggtcgccgac gaagcggtag gccttggcca gcgccgccgg gccgatgaac
     1681 tcggggtcgg cttccattga gaggcaggcg gagacgcagg agccgcactg gatgcaggcc
     1741 atcggctggg tgatgtcgag catcgattcc ggctcgacca cgtactcgcg ctctggcggc
     1801 gcgccgtgcg gcagcagcca gggggtgacg cggcggatct tggcccagtg ggtcgactcc
     1861 atgtcggtga ccaggtcctt gatcaccggc atgttgccca tcggctcgat cacgaccacg
     1921 ttgccggcgc tctcgccgcc gaccgcgaag gctttcttgt cggccagctc ctgggcctcg
     1981 ccgatctggg tcttgcagcc gagcccggac tggccgttga tcttcatccc gcaggagccg
     2041 cagatcgcgg cgcggcagga gcagcgcacg gcgagcgagc cgtcctcgcg gtccttcgcc
     2101 tgcaaaaggg cgtcgagaac cgacagtgag ggctcgagct cgacgttgaa ctgctcccag
     2161 tagggaccct ggccgctctc cggctggaat ctgcggactt tgatcgtgta atcggccatc
     2221 agtaggtcct cgcttccggt tgccactggg tgatggtcac ctcggagtat gagatctccg
     2281 ggtcgccatc ggttttgacg aggtcgatgt gtttgagcca ctcctcgtcg tttcgctccg
     2341 ggtagtcggt gcggtactgg gcgccgcggc tctccttgcg ctccagcgcc gcgatgcagg
     2401 tgcactcggc attgtcgagc atgtagccga gctcgatcgc ccccagcagg tcctggttga
     2461 agacgctgcc gtggtcgtcg atgtaggcgg tcttcgcctc ctccttcagc cgtctaacaa
     2521 tctccagcgc ttcctggatc ccggcctcgt cgcggaagac ggcgactttg ccgtccatcg
     2581 tctggcccag ctcgagcttg atctcggaga cgcggcggcc ggtgcccttc tcgcgcgagt
     2641 tgatgtcggc gatcatcgcc tcctcgtcct cgagcacctg cttcgagggc ctggtgccct
     2701 cgatccgctt cgcccgcgag gcggcgtcgc gcccggagcg cttgccgaag atcagcgtgt
     2761 cgagcagcga gttggcgccc agccggttgc cgccgtggac ggagacgcag gcggtctcgc
     2821 cggccgcgta gaggccgggg atgtcggtgc gaccgttgat gtcggtcttc acgccaccca
     2881 tcgtgtagtg gttacccggc ttgatgtgga tcggttcgcg ggtgatgtcg acgccggcga
//
LOCUS       DASRIG010000424         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3064609, whole genome shotgun sequence.
ACCESSION   DASRIG010000424 DASRIG010000000
VERSION     DASRIG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064609"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFB52_11330"
     CDS             <1..210
                     /locus_tag="VFB52_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZO06970.1"
                     /translation="VVGKRVGGLGGHMLGTTFMGEGGVIDENLRHHQLANLYVAGGSS
                     FPTHSALHPTTTIAALAIRLGRHLA"
     gene            207..1472
                     /locus_tag="VFB52_11335"
     CDS             207..1472
                     /locus_tag="VFB52_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZO06971.1"
                     /translation="MIGLLRNEAVRKRILAPISYDALMLVMNLITGVIVARTLGADGR
                     GQLAATLVLVLTATWLFSVGSTEAISFHQAKNRRDAARLIGSWLAVVGVASVVAILVM
                     ELVLPVLFSAQTDSAIEVARWYIPLIAVSLAIAMYNGVLLGDEDFTAYNVLRAVIPVG
                     IAIGYGILLLFDEFTVEGALVANGAATTVAALGMAWRTGRRHGIAKPDIPLLRSTLWY
                     GLRAHGGSIAGFVNARLDLLILPAFLAAESVGLYSVATNTTSIIGALTGTVAMFVLPV
                     AARRKHGSARTVVKTLQATAMIGLVLAIPMIVLAQFGLELVYGEEFGQAATAMRIMLP
                     GEVLDACSVVLWAGLLAANRPFLSSAAAGPGAVLTIVGLLLFLESGGIDAAAIVSTSV
                     HTLVFIVSISLYRHVQGLRWRDFLASPTA"
     gene            1469..>2253
                     /locus_tag="VFB52_11340"
     CDS             1469..>2253
                     /locus_tag="VFB52_11340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO06972.1"
                     /translation="MILSLLVEQLPNLPARNEEWQALGIAAGNPFTTWEWASAWWREF
                     GEERRQLILGCRDDEGALVGVLPLYLASRHPRTLRIVGNGAADQLGPVCRPEHREAVA
                     AALRGALAERPGWTVCATERLAVGDGWPQLLGGHRTRRERSLTIRPRTADWDEFLSGY
                     SANFRGQVRKFERRLERDHDLRYRIADDPERVDADIALFLDLHAARWGDRSQVYAGGL
                     EEFHRELARLALAEGFLRLSFLELDGEPCAGIHAFRLGGVDWI"
BASE COUNT          291 a          839 c          750 g          373 t
ORIGIN      
        1 gtcgtcggca agcgcgtcgg cggcctcggc ggccacatgc tcgggaccac cttcatgggc
       61 gagggcggcg tgatcgacga gaacctccgc caccaccagc tggccaacct gtatgtcgcg
      121 ggcggctcct cgtttccgac ccacagcgcg ctgcacccga cgacgacgat cgcggctctg
      181 gcgatccggc tcgggcgtca cctcgcgtga tcggacttct gcgcaacgag gctgttcgca
      241 aacgcatcct cgcgcccatc tcctacgacg cgctgatgct ggtgatgaac ctgatcaccg
      301 gcgtgatcgt tgcccgtacg ctcggtgccg acggccgcgg tcagctcgcc gcgaccctgg
      361 tcctcgttct caccgcgacc tggctgttct cggtcggcag caccgaggcg atctcgttcc
      421 accaggccaa gaaccggcgc gacgcggcgc ggctgatcgg ctcctggctg gccgtcgtcg
      481 gcgtcgccag cgtcgtcgcg atcctggtca tggagctcgt cctgccggtc ctgttcagcg
      541 cccagaccga ctcggcgatc gaagtcgcgc gctggtacat cccgctgatc gcggtctcgc
      601 tggcgatcgc gatgtacaac ggcgtgctgc tcggcgacga agacttcacc gcctacaacg
      661 tgctgcgggc ggtgatcccg gtcggaatcg cgatcggcta cgggatcctc ctcctgttcg
      721 acgagttcac ggttgagggg gcactggtcg cgaacggcgc cgcgaccacg gtcgccgccc
      781 tcgggatggc ctggcgcacc ggccgccgcc acgggatcgc caagcccgac ataccactgc
      841 tgcggagcac gctctggtac gggctgcgcg cccacggcgg ctcgatcgcc ggtttcgtca
      901 acgctcgcct cgacctgctg atcctgcccg ccttcctcgc cgccgagtcg gtcggtctct
      961 actcggtcgc gaccaacacc acctcgatca tcggcgccct gaccgggacc gtggcgatgt
     1021 tcgtgctgcc ggtcgccgcg cgccgcaaac atggctcggc gcggaccgtg gtcaaaacgc
     1081 tgcaggcgac ggcgatgatc gggctggtgc tggcgatccc gatgatcgtc ctcgcccagt
     1141 tcgggctcga actcgtctac ggcgaggaat tcgggcaagc ggcgacggcg atgcgaatca
     1201 tgctccccgg cgaggtgctc gacgcctgct cggtcgtgct ctgggccggg ctgctcgccg
     1261 ccaaccgtcc cttcctctcc agcgccgccg ccgggccggg cgcggtgctg acgatcgtcg
     1321 ggctgctgct cttcctcgaa agcggcggca tcgacgcggc cgcgatcgtc agcacctcgg
     1381 tccacacgtt ggtcttcatc gtctcgatct cgctctaccg ccacgtgcag gggttgcgct
     1441 ggcgcgactt cctcgcctcg ccgaccgcat gatcctctcc ctcctggtcg aacagctgcc
     1501 caacctgcct gcccgcaacg aggagtggca ggcgctcggg atagccgccg gcaacccgtt
     1561 cacgacctgg gagtgggcga gcgcctggtg gcgcgagttc ggcgaggaac ggcgccagct
     1621 gatcctcggc tgccgcgacg acgagggtgc cctggtcggc gtccttcctc tctatctcgc
     1681 ttcacgccac ccgcgcaccc tgcgcatcgt cggcaacggc gccgccgacc agcttggccc
     1741 ggtctgccgg cccgagcacc gcgaggcggt tgccgcggcg ctccgcgggg cgctcgccga
     1801 gcgccccggc tggaccgtct gcgcgaccga gcggctggcg gtcggcgacg gctggccgca
     1861 gctgctcggc ggccaccgga cgcgccgcga gcgcagcctc acgatccgcc cacggaccgc
     1921 cgactgggac gagttcctct ccggctacag cgccaacttc cgcggccagg tgcgcaagtt
     1981 cgagcggcgg cttgagcgcg accacgatct gcgctaccgg atcgctgacg accccgagcg
     2041 agtggacgcg gacatcgccc tgtttctcga cctgcacgcc gcccgctggg gggaccgctc
     2101 gcaggtctac gccggcgggc tcgaggagtt ccaccgcgag ctggcgcggc tggcgctggc
     2161 agagggcttt ctgcgcctct ccttcctgga gctcgacggc gagccctgcg ccggcatcca
     2221 cgccttccgg ctcggcggtg tcgactggat cta
//
LOCUS       DASRIG010000425         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2526618, whole genome shotgun sequence.
ACCESSION   DASRIG010000425 DASRIG010000000
VERSION     DASRIG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2526618"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="metK"
                     /locus_tag="VFB52_11345"
     CDS             <1..785
                     /gene="metK"
                     /locus_tag="VFB52_11345"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZO06973.1"
                     /translation="RLAEVRKAGVVPYLRPDGKTQVTVRYENGRPVEVVKVLISAQHA
                     PDLDAESLIKPDLWEHVVEPVLHEGYKELFTERDLLPNLLVNPTGRFEIGGPMGDAGL
                     TGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRCEIQVA
                     YAIGVAHPVSLMVETYDTGRVSNADIVRAISEVFDLRPAAFRRYLDLHRPIFQKTAAY
                     GHFGREDHDFTWERTDRADALRAAAGMAAPGNQAGVEAGVPG"
     gene            complement(1098..1541)
                     /locus_tag="VFB52_11350"
     CDS             complement(1098..1541)
                     /locus_tag="VFB52_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZO06974.1"
                     /translation="MLVIAFLVGLGTNIANGDTDTGLVILVAVVGVAIVILAGFIKRV
                     TTHYTITTRRLHIQRGIISRDIQETRLERVQNVNYNQSLLERVLQVGDVDFDTAAGDD
                     YNFIFVGVADPAEVVESVDRATHATSPTGLGDQPAQQQAPQGPAG"
     gene            1707..>2059
                     /locus_tag="VFB52_11355"
     CDS             1707..>2059
                     /locus_tag="VFB52_11355"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06975.1"
                     /translation="MAIAKVEPLLTARALRGPFDYRLPDHLGDVGIGSLLRIPFGRQR
                     VIGVVVDVGESSDLPPDRLAEPLEALEAGATPALVRLGLWVAHQYCSTPARGLELVLP
                     PGTGRTGRVPSPQLEL"
BASE COUNT          311 a          690 c          728 g          330 t
ORIGIN      
        1 agcgcctcgc cgaggtacgc aaggcggggg tcgttccgta tctgcggccc gacgggaaga
       61 cccaggtcac ggtccgctat gagaacggcc gcccggtgga ggtcgtgaag gtcctcatct
      121 cggcccagca cgccccggac ctcgacgccg agtcgctgat caagccggac ctctgggaac
      181 acgtcgtcga gcccgttctg cacgaggggt acaaggagct cttcaccgag cgggacctcc
      241 tgcctaacct cctcgtcaac ccgaccggcc ggttcgagat cggcgggccg atgggcgacg
      301 ccggcctcac cggccgcaag atcatcgttg acacctacgg gggcgctgcg cgccatggtg
      361 gaggcgcgtt ctccggcaag gacccctcca aggtcgatcg ctcagctgca tatgcggccc
      421 gctatgtcgc gaagaacgtc gtcgccgccg gactcgccga ccgctgcgag atccaggtcg
      481 cctacgcgat cggagtcgcc catcccgtct cgctgatggt cgagacctat gacaccggcc
      541 gagtctcgaa cgcggacatc gtgcgcgcga tcagcgaggt cttcgatctg cgccccgcgg
      601 cgttccgccg ctacctcgac ctgcatcgcc cgatcttcca gaagaccgcc gcgtacgggc
      661 acttcggccg cgaggaccat gacttcacct gggagcgcac cgaccgagcg gacgcgctgc
      721 gcgccgccgc gggcatggcg gccccgggca accaggccgg ggtcgaggcg ggggtgccgg
      781 gctagcgcac cggcgatcgc cccggcgttt gcgacgaggt ggggcggctg aagggcaagt
      841 agggggagcg cacacactct ccgctcgctt tcggcgctcc attggcaccg aatcgaaccg
      901 agagaaggat tgaccacgtt atgggtggtg aaatcccact ctcgccggga gaaagcgatc
      961 gagtgagcga ttggggccct tacaggtccc gaatcactca ctcgccggcc gggtccttcc
     1021 gtcgttgccc gggacgacgc atcgccgcgg ggttcggtcg ggtgcggccg atgcggacgc
     1081 gccctcgcgg agggcgttca gcccgcgggt ccctgtggcg cctgctgttg cgccggctga
     1141 tcgccgaggc cggtcgggga cgtcgcgtgc gtggcgcgat ccacgctctc cacgacctcg
     1201 gccgggtcgg cgacgccgac gaagatgaag ttgtagtcat cgccagcggc ggtgtcgaag
     1261 tcgacgtcgc ccacctgcag gacgcgctcg agcagcgatt ggttgtagtt cacgttctgg
     1321 acgcgctcca gccgcgtctc ctggatgtcc cgcgagatga ttccgcgctg gatgtggagg
     1381 cgtcgcgtcg tgatcgtgta gtgcgtcgtg actcgcttga tgaacccggc gaggatgacg
     1441 atcgcaactc cgacgaccgc gaccaggatc accaggccgg tgtcagtgtc gccatttgcg
     1501 atgttggtcc cgaggccgac caggaaggcg atcaccagca ccgccagcag gcccttgatg
     1561 tagaagccga ggatctagcg ccaggaagga tggccctgga acatgatctg ctcgccgggg
     1621 gcgaggcgag gcgccattgc cgaagcatag gttgtggcgc ctcgtcgaag gcggccgccg
     1681 gggcgaccac tagcttccgc acggacatgg cgatcgcgaa ggtcgagccc ctcctgacgg
     1741 cccgggcgct gcgcggcccg tttgactacc ggctgcccga ccacctgggc gacgtgggta
     1801 tcgggtcgct gttgcgcatc ccgttcgggc ggcagcgggt catcggggtc gttgtcgatg
     1861 tcggtgagtc gagcgacctg cctccggacc gcttggcgga gccgctcgag gcgctcgagg
     1921 ctggcgcgac ccccgcgctg gtgcggctgg gcctgtgggt cgcgcaccag tactgctcga
     1981 ctccggcgcg aggcctggag ctggtgctgc cgccgggtac cgggcgtacc ggtcgcgtcc
     2041 caagtccgca gctcgagct
//
LOCUS       DASRIG010000426         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2815613, whole genome shotgun sequence.
ACCESSION   DASRIG010000426 DASRIG010000000
VERSION     DASRIG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815613"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFB52_11360"
     CDS             complement(<1..488)
                     /locus_tag="VFB52_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06976.1"
                     /translation="MEYVLLLVSFAVVLAGALLFTNAIEWIGHKLHLGEGAVGSILAA
                     VGTAMPETLIAIVALISVDEGSEDVAIGAIVGAPFLLGTLAMGLVGLFAYLYRDRRKT
                     GLHLDTHGPTLERDLLFFLGFFGLAGLLAWGPPSGVRIVFGIVFLLAYVLYVARTLRS
                     SG"
     gene            complement(533..1648)
                     /gene="cax"
                     /locus_tag="VFB52_11365"
     CDS             complement(533..1648)
                     /gene="cax"
                     /locus_tag="VFB52_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZO06977.1"
                     /translation="MRPALNASFFRTPQGWPYLLVPLIPIAVLLELVHAEATIVFAAS
                     ALGIIPTAALMGRATEELAARSGPGIGGLLNVTFGNAPELIIALFALNQGLQEVVKAS
                     VVGSILGNILLVLGAAMLAGGIGRDKQTFSATGASVQSTMLMLAAAALLMPAIFELVE
                     GQGLPQPGAESINYGPTVEHLSLAVALVLIATYAAGLFFSLKTHRSLFNPEYEEEDTW
                     GWSTRKAVGALAIAGVLVGVMSEVLVGSISEASESIGLSEFFVGVIVVAIVGNAAEHW
                     VAVLVAMKNKMDLAVNIAIGSSAQVALFVAPVLVIASFFLGDFPLALVFNGFELGAIL
                     LAILIANHVTQDGESTWFEGVQLLAVYFVFGLAFYFA"
     gene            complement(1658..2785)
                     /locus_tag="VFB52_11370"
     CDS             complement(1658..2785)
                     /locus_tag="VFB52_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06978.1"
                     /translation="MSRIRIFVLLATVVALAGVLAACGGDSDSSGEDPQGVIENATLE
                     GVKSGDVSLSIGIVSEGGEDSGDLKLSLSGPFQSRGNETLPELGLDVTANGNVTGDAI
                     DFDGGLTLLSDRAFVRYNGTEYEVDPTTFGFVKSGFEQGFAEGSGGSATSPTACQEAV
                     SAIDLNTVIENLSNEGSADVDGTSTTKVSGDLNPDGAIDAIIALTEDPACASTLEAAG
                     PLPLDELEQAKGEISKALKEAHIEVYVGDDNIIRKVVTEITVEPKTADGEKVDLDFEM
                     SIGGVNEKQTIEAPANAEPLEGLFKELDVDPMALLEAGSGGGLDGLLEGVTGAEGGVD
                     GGEVEIEPEDLPDVEESTAYLECLGEATTANDLQKCAQLIE"
     gene            complement(2836..>3115)
                     /locus_tag="VFB52_11375"
     CDS             complement(2836..>3115)
                     /locus_tag="VFB52_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06979.1"
                     /translation="SLPPRGDDNQAHVPDLAPPLAELLSTKPGPYIALMLAGFAIGIL
                     GHLFKVKWLVAAGVILIFLGALLFPLAANLTSDDRPPPIEESRTGDSG"
BASE COUNT          529 a          995 c         1085 g          506 t
ORIGIN      
        1 tcgccgctgg agcgcagggt gcgggcgacg tagaggacgt aggcgagcag gaagacgatg
       61 ccgaagacga tccgcacccc gctcggaggg ccccaggcga gcagcccggc gagcccgaag
      121 aagccgagga agaagagcag gtcgcgctcc agggtcggcc cgtgggtatc gaggtgaagg
      181 ccggttttgc ggcggtcccg gtagaggtag gcgaacagcc cgaccaggcc catcgccagg
      241 gtgccgagca ggaacggggc gccgacgatc gcgccgatcg cgacgtcttc ggagccctcg
      301 tcgacgctga tcaaggcgac gatcgcgatc agcgtctccg gcatcgcggt cccgaccgcg
      361 gcgaggatgc tgccgaccgc cccctccccc aggtgcagct tgtgcccgat ccactcgatc
      421 gcgttggtga acagcagggc gccagcgagg acaacggcga agctgacgag gaggagaacg
      481 tattccacgc cgccaggcta ttggaccggc ccggtcgggc tccgcgtggt cctcaggcga
      541 agtaaaacgc gaggccgaag acgaagtaga cggcgagcag ctgcacgccc tcgaaccagg
      601 tcgactcgcc gtcctgggtg acgtggttgg caatcaggat cgccagcagg atcgccccca
      661 gctcgaagcc gttgaagacc agcgcgagcg ggaaatcgcc gaggaagaag gaggcgatca
      721 ccagcaccgg ggcgacgaag agggcgacct gggcgctcga gccgatcgcg atgttgaccg
      781 ccaggtccat cttgtttttc atcgcgacca ggaccgcgac ccagtgctcg gcggcgttgc
      841 cgacgatcgc gacgacgatc acgccgacga agaactcgga gaggccgatc gactccgagg
      901 cctcgctgat cgagccgacc aggacctcgg acatcacgcc gacgaggacg ccggcgatcg
      961 ccagcgcccc caccgctttg cgggtcgacc agccccaggt gtcctcctcc tcgtactcgg
     1021 ggttgaacag cgagcggtgg gttttcagcg agaagaagag gccggctgcg taggtcgcga
     1081 tcaggacgag cgcgacggcc agcgagaggt gctcgacggt cgggccgtag ttgatcgact
     1141 cggcgccggg ctggggcagg ccctggccct cgaccagctc gaagatcgcc ggcatcaaga
     1201 gcgccgcggc ggccagcatc agcatcgtgc tctgcacgct ggcgccggtg gcgctgaagg
     1261 tctgcttatc gcggccgatg ccgccggcca acatcgccgc cccgagcacg agcaggatgt
     1321 tgccgaggat cgagccgacg accgaggcct tgaccacctc ctgcagcccc tgattgagcg
     1381 cgaacagggc gatgatcagc tccggcgcgt tgccgaaggt gacgttcagc aggccgccga
     1441 tgccagggcc cgagcgggcg gcgagctcct cggtggcgcg gcccatcaac gccgcggtgg
     1501 gaatgatccc gagcgccgaa gcggcgaaga cgatcgtcgc ctcggcgtgg accagctcga
     1561 gcaggaccgc gatcgggatc agcgggacca gcaggtaagg ccagccctgc ggcgtgcgga
     1621 agaacgacgc gttcagggct ggcctcacgt cagagcgcta ttcgatcagc tgagcgcact
     1681 tctgcaggtc gttggcggtg gtcgcttcgc cgagacattc gaggtacgcg gtcgattctt
     1741 cgacgtccgg caggtcttcc ggttcgattt cgacttcgcc gccgtcgacg ccgccctcgg
     1801 cgccggtgac gccttcaagc agcccgtcga gaccgccgcc gctgccagct tccagcagcg
     1861 ccatcgggtc gacgtcgagc tctttgaaca ggccttcgag cggctcggcg ttggccggtg
     1921 cctcgatcgt ctgtttctcg ttgacgccgc cgatcgacat ctcgaagtcg aggtcgacct
     1981 tttcgccgtc cgcggttttc ggttcgaccg tgatttcggt gaccactttg cggatgatgt
     2041 tgtcgtcacc gacgtagacc tcgatgtgcg cctctttcag cgctttcgag atttcgccct
     2101 tggcctgttc gagttcgtcg agcggcagcg ggccggccgc ttccagcgtc gaggcacagg
     2161 ccgggtcttc ggtcagcgcg atgatcgcgt cgatcgcgcc gtcggggttg aggtcaccgc
     2221 tgaccttggt ggtcgaggtg ccgtcgacgt cggcgctgcc ttcgttgctg aggttttcga
     2281 tgaccgtgtt gaggtcgatc gccgagaccg cttcctggca ggccgtcggc gaggtcgcag
     2341 accctccgct gccttcggcg aagccctgtt cgaagccgga cttgacgaat ccgaaggtgg
     2401 tcgggtcgac ttcgtactcg gtcccgttgt agcggacgaa ggcgcggtcg gagagcagcg
     2461 tgaggccgcc gtcgaagtcg atcgcgtcgc cggtgacgtt gccgttcgcg gtcacgtcga
     2521 ggccgagttc gggcagcgtt tcgttgccac gactctggaa cgggccggag aggctgagtt
     2581 tcaggtctcc gctgtcttcg ccgccttcgg agacgatccc gatcgagagg ctgacgtcgc
     2641 cgcttttgac cccttccagg gtcgcgtttt cgatgacccc ctgcgggtcc tcgccactgc
     2701 tgtcactgtc accaccgcaa gcggcgagca cccctgccag ggcgaccacg gtcgcgagca
     2761 gtacgaagat gcgaatacga ctcaagggat acagctcctc tgcattcggg aacgaagtga
     2821 gccctgatcg tagcctcagc cgctgtcgcc cgtccgcgat tcttcgatcg gcggcggacg
     2881 atcgtccgaa gtgaggttgg cggcgagcgg gaacagcagg gcgccgagga agatcaggat
     2941 cacgcccgcg gcaaccagcc atttgacctt gaaaaggtgg cccagaatgc cgatcgcgaa
     3001 gcccgccagc atcagggcga tgtaggggcc gggcttggtc gagagcagct cggcgagagg
     3061 cggcgcgagg tcgggcacgt gtgcctgatt atcatcgccc cgtggcggaa ggctc
//
LOCUS       DASRIG010000427         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2272080, whole genome shotgun sequence.
ACCESSION   DASRIG010000427 DASRIG010000000
VERSION     DASRIG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272080"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..885
                     /locus_tag="VFB52_11380"
     CDS             175..885
                     /locus_tag="VFB52_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO06980.1"
                     /translation="MGTFAKFTSGRPKALAIGDDAEIRRDLRGLLAAADFQLVEAGDG
                     GNGLRVLFDERPDVVVLDLELADTDGFAVITKIREVSDVPLLAIAGDGASERVRALRT
                     GADDCVPKPIGHELVARVEALLRRPRPTVEVAAIIEDEFIQIDRMRHRVEALGIEVPL
                     TPTEFRMLGAFAENPGRVLGHGQLLDMVWGDRIRAKDEVKLYVSYLRRKLGAAGVDPV
                     ETVRGVGYRYQPRHAEEA"
     gene            1257..1652
                     /locus_tag="VFB52_11385"
     CDS             1257..1652
                     /locus_tag="VFB52_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06981.1"
                     /translation="MSGPAGNPGAPGGSGGQGPAGPQGAPGKNGANGLDGLVGPTGPA
                     GQVPQTLVETVESQNQQIETLEKQVTTLSGQLSDVQTTASKACAQANAATTQVNSVVD
                     VVGGLTLSGVLGGLLNIPKLPALLPTVSC"
     gene            complement(1662..1868)
                     /locus_tag="VFB52_11390"
     CDS             complement(1662..1868)
                     /locus_tag="VFB52_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06982.1"
                     /translation="MSDAIEHGFKEGDKVWVQDADGNHHPGVFVGENEAAAWFGGGPS
                     AYVVHPEAHQAEVVSLFRITPRDE"
     gene            1985..2116
                     /locus_tag="VFB52_11395"
     CDS             1985..2116
                     /locus_tag="VFB52_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06983.1"
                     /translation="MGGLIAVLIVVALALVLLKVVVAGGILGLIALVLLILLLLGRI"
     gene            complement(2113..2823)
                     /locus_tag="VFB52_11400"
     CDS             complement(2113..2823)
                     /locus_tag="VFB52_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HZO06984.1"
                     /translation="MRSLAKVGIAALAAALVCLVVAAGVGGATGTTGTGAGAQAQTSS
                     ASPPPSNPWPIPFGPKRKREMADYSERHYGQHRWRLRNPKVIVEHMAQTPTAEALFNT
                     FAHDNPDPELGELPNVCAHYGISGSGKIFRFVNLRTRCRHTVGLNWTAIGIEHVGYGD
                     GDLMGDRSQLRASLRLTQYLRCRFDIGVRNVIGHNESLSSPYHYERVPSLRTQTHGDM
                     DSSSMRVYRQKLRRLGAC"
     gene            2859..3806
                     /gene="ligD"
                     /locus_tag="VFB52_11405"
     CDS             2859..3806
                     /gene="ligD"
                     /locus_tag="VFB52_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZO06985.1"
                     /translation="MASAGEPMKAKLGATPFSDPDWVFERKLDGIRAIAERHGDVVTL
                     TSRTGRDLSRSYPELVAALAAEAATDFVADGEIVAFDGSRTSFAKLQGRIHLQDPRPE
                     LIASVPVHLYLFDLLRFDGHDLTGLPLRARKGALRRAFDFHGPVRYTPHRNEHGERFF
                     EEACAKGWEGLIAKRAESQYTHRRSGDWIKLKCSREQELVIGGFTPPGGARKHFGALL
                     IGYYEDGELRFAGKAGTGFSERTLTELGARLEALEIPESPFTRGSGLPRVARWVKPEL
                     VGQFAFSEWTRDGKLRHPSFVGLRVDKPAREVVRETPGA"
     gene            complement(3857..4321)
                     /locus_tag="VFB52_11410"
     CDS             complement(3857..4321)
                     /locus_tag="VFB52_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06986.1"
                     /translation="MDDELTELLEVGHRLQMTGERRTVVGTLELEERIADAVGVHRFD
                     RHRGSQSRVELPLQHRPRGEQPGVPLPARVDAGEGAVVQLVTEVEGELEVVIGERVGH
                     FLTEGRDRLRRDGVTHGSHRPREQFADQLADRSVRMQSRRSRRLLRGHQGTH"
     gene            4360..4902
                     /locus_tag="VFB52_11415"
     CDS             4360..4902
                     /locus_tag="VFB52_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZO06987.1"
                     /translation="MGDPAPAGAPKTALLEVQHDEYGGGRAERMHSALFAKTMRGLGL
                     DDAENAYLERVPGVTIATVNLMSRFGLHASRRGAIVGHLAMFEMTSARPNRRYGNALR
                     RLGFGPEVTDFYDEHVEADAVHENIAAYDLAGGLARAEPELASDILFGARALLYLEDR
                     FARHLLGCWEQGRTSLRQSL"
     gene            4899..5945
                     /locus_tag="VFB52_11420"
     CDS             4899..5945
                     /locus_tag="VFB52_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06988.1"
                     /translation="MNAAYVLPLRWSEPGPLAELSRYLRWLAGEVTEVLVIDGSPDDL
                     FAAHSLAFGSDVRHCRPDPDLDFPMGKVNGVVTGLRACSCERVVIADDDVRYDGQTLR
                     RTLEMLTGADLVRPQNYFDELPWHARWDSARSLLNRVFTGDPGFPVGDFPGTLALRRS
                     TFLRAGAYDGEALFENLELMRTIRAAGGSVLTPLDLYVARRPPGSAHFRSQRVRQAYD
                     DFAIPLRIGGFLAIGPLTGYLALRRRWWTLGAAVLASIGAAEAGRRRAGATARFPPSG
                     SLLAPAWLAERAVCSWLAVGARLRGGVRYGGGRLRLAAHRQRALRRRYSESGSGGPTG
                     PGEASLSDRNPIVL"
     gene            complement(5867..6112)
                     /locus_tag="VFB52_11425"
     CDS             complement(5867..6112)
                     /locus_tag="VFB52_11425"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZO06989.1"
                     /translation="MANEPTKITPYRNGPYLVRGSFVVCDQDGNEIESQRPVIALCRC
                     GKSQIRPFCDGTHKTIGFRSESEASPGPVGPPEPDSE"
     gene            6303..>6745
                     /locus_tag="VFB52_11430"
     CDS             6303..>6745
                     /locus_tag="VFB52_11430"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HZO06990.1"
                     /translation="MIMRLDRLQAEIPPPSTPDPVGASVVQELLGGKFGEMSTFMNYT
                     YQSFNFRNRQGARPFYDLIASIAAEEFGHIELVSTAINTMLTGAAKGKAGKKGALKGV
                     KDTRNTQQFIAGGASAMVQDSMGKPWSGDYVNATGDLIGDLTHNF"
BASE COUNT         1032 a         2276 c         2324 g         1113 t
ORIGIN      
        1 gattggagcg cgcggatatg gagcgcgttg ctaaccgtaa ggtttcgtaa cgctgggctg
       61 aacggtgaaa agccctggct ttcactatta ctaattccag catttttcag acttaaggtt
      121 tcgttacatg aatttgacaa agggctctga gtaaaggaac ttcctactag cgaggtggga
      181 acgttcgcca aattcacgag cggccgaccc aaggcgctgg caataggaga cgacgcggaa
      241 ataaggcggg acctacgggg actgctggcg gcggcggatt tccagctcgt ggaagccggt
      301 gacggaggaa atggcctgag ggtgctcttc gatgagcgcc ccgacgtcgt tgtgctcgac
      361 ctcgagttgg ccgacaccga tggatttgcc gtgatcacga agatccgcga ggtcagtgac
      421 gtgccgctgc tggcgatcgc cggcgatggt gccagcgaac gggtgcgagc gctgcgaaca
      481 ggggccgacg actgtgttcc caagccgatc ggccacgaac tcgtcgcccg cgtggaagcg
      541 cttctgcggc gcccacggcc gacggtcgaa gttgccgcga tcatcgaaga cgagttcatc
      601 cagatcgacc gcatgcgcca ccgggtcgag gcgctgggga tcgaggtgcc gctcaccccg
      661 accgagttcc ggatgctcgg tgccttcgct gagaaccccg gccgggtgct cggtcacggt
      721 cagctgctcg acatggtctg gggcgatcgg atccgcgcca aagacgaggt gaagctctac
      781 gtcagctacc tacggcgcaa gctcggggca gctggtgtgg accccgtgga gacggtgcgc
      841 ggggtgggtt accgctacca gccgcgccac gccgaagagg cgtaaccgct tgtagggcgc
      901 cactcatacg attttcttac cgtaaggatt tggttcacac cttcatccca ccgctggaga
      961 gatttcccga gtactcatac tttgctctaa cgacgttcat ccgcgattcc gatcgtggct
     1021 gccgaaaagg ccacatgcga aacccgcgca cgtcagcagc gatcatggtg gcggccgtgt
     1081 ccgtgctcct cctctcgggg ggagccgcgg aggcggcgaa gtcaccggtg tccggcagcg
     1141 tcctcaccgc ttgcctgaag acgaagggga agccgtctac ccgcggcacc ctccgcgtcg
     1201 tgccctcagc ccgcgcttgc aagaagaagc ggggcgagag ggcgatcgcc tggaacgtga
     1261 gcgggccggc agggaacccg ggggcgccag gcggcagtgg agggcaaggg cccgccgggc
     1321 cgcagggtgc cccgggcaaa aacggggcca acggcctcga cggcttggtc gggccgaccg
     1381 gccccgctgg tcaggtcccg cagacgctgg tcgaaacggt cgaatcgcag aaccaacaga
     1441 tcgaaaccct ggagaaacag gtgacgacgc tgtccggcca gctcagcgac gttcagacca
     1501 ccgccagcaa agcctgtgcg caggcgaacg cggcgaccac ccaggtcaac tcggtggtcg
     1561 acgtggtcgg gggcctgacc ctgagcgggg tgctcggcgg cctgctcaac attccgaaac
     1621 tgccggcgct gctgccgacc gtctcctgct gagtcgcgcg gctactcgtc gcgcggggtg
     1681 atccggaaga gcgagacgac ctcggcctgg tgcgcctcgg gatggacgac gtaagcgctg
     1741 ggaccgccgc cgaaccaggc ggcagcctcg ttttcgccga cgaagacgcc cggatggtgg
     1801 ttgccatcgg cgtcctgcac ccagactttg tcgccctcct tgaacccgtg ttcgatcgcg
     1861 tcgctcatcg ccgccagtct attcgccgcc gccggacgat ctggtttctc gcatcgacgc
     1921 ctgggtagcg ggtgggcgct cgcagggaaa atcgctggaa atgaagaaag ggcggggtcg
     1981 gatggtgggt gggctgatcg ctgtgctgat cgtcgtcgcg ctggcgctgg tgctgttgaa
     2041 ggttgtggtg gcgggcggaa tcctcggctt gatcgccttg gtcctcctga tccttctctt
     2101 gctcggccgg atctagcagg ccccgagccg gcgcagcttc tggcggtaga cccgcatcga
     2161 ggagctgtcc atgtcgccgt gggtctgcgt gcgcaggctc ggcacccgct cgtagtggta
     2221 aggcgagctg aggctctcgt tgtggccgat cacgttgcgg acgccgatgt cgaagcggca
     2281 gcgcaggtac tgggtgaggc gcagcgaggc gcgcagctgg ctgcggtcgc ccattagatc
     2341 cccgtcgccg tagccgacgt gctcgatccc gatcgcggtc cagttcagcc cgaccgtgtg
     2401 gcggcagcgg gtacgcaggt tgacgaagcg gaagatcttg ccgctcccgg agatcccgta
     2461 gtgggcgcag acgttgggca actcgcccag ctccgggtcg gggttgtcgt gcgcgaaggt
     2521 gttgaagagc gcttcggcgg tcggggtctg cgccatgtgc tcgacgatca ccttcgggtt
     2581 gcgcagccgc cagcggtgct ggccgtagtg gcgctcggag tagtccgcca tctcccgctt
     2641 gcgtttcggg ccgaagggga tcggccaggg gttcgagggc ggcggcgagg cggagctggt
     2701 ctgcgcctga gcgccggcgc cggtgcccgt cgtacccgtg gcgccgccga ccccggccgc
     2761 gaccacgagg cagaccaatg ccgccgcgag cgcggcgatc ccgaccttgg cgagggacct
     2821 catcgccgcg catggtacgg gccacgtagg ctgggtcgat ggcgagcgcg ggcgagccga
     2881 tgaaggcgaa gctcggcgcc actccgttca gtgaccccga ctgggtcttc gagcgcaagc
     2941 tcgacggcat ccgcgcgatc gccgagcgcc acggcgacgt cgtcaccctg acctcgcgca
     3001 ccggccgcga cctctcgcgc tcctatccgg agctggtcgc cgcgcttgcc gccgaggccg
     3061 cgaccgactt cgtcgccgac ggcgagatcg tcgccttcga cggcagccgg accagcttcg
     3121 cgaagctgca gggccggatc cacctgcagg acccgcgccc ggagctgatc gcctcggtcc
     3181 cggtccacct ctacctcttc gacctgctgc gcttcgacgg ccacgacctc accggattgc
     3241 cgttgcgcgc ccgcaagggg gcgctgcgtc gtgccttcga cttccacggc ccggtccgct
     3301 acacgccgca ccgcaacgag cacggcgagc gcttcttcga ggaggcctgc gcgaagggct
     3361 gggagggcct gatcgccaag cgcgccgaga gccagtacac ccaccgccgc agcggcgact
     3421 ggatcaagct caaatgctcg cgcgagcagg agctggtgat cggcggcttc accccgccgg
     3481 gtggcgcacg caagcacttc ggggcgctgc tgatcggcta ctacgaggac ggcgagctgc
     3541 gcttcgccgg caaagccggg accgggttca gcgagcgcac cctgaccgag ctgggcgccc
     3601 ggctggaggc cctcgagatc cccgagtcgc cgttcacccg cggcagcggc ctgccgcggg
     3661 tggcgcgctg ggtgaagccg gagctggtcg gccagttcgc cttctccgag tggacccgcg
     3721 acggcaagct ccgccacccg agcttcgtcg gcctgcgggt cgacaagccg gcccgcgagg
     3781 tcgtgcgcga gacgcccgga gcgtaggtgc ccccttgatc gggtagctcg aggtgacgta
     3841 cttcgagcgg agggaatcaa tgggtgccct ggtggccacg gagaagccgg cgagatcgcc
     3901 ggctttgcat tcgaacggac cgatcagcga gttgatctgc gaactgctcg cgcggccggt
     3961 ggctgccgtg ggtgacaccg tcacgtcgga ggcgatcgcg cccctcagtc aggaagtggc
     4021 cgacccgctc accgatgacg acttccaact cgccctctac ctctgttacg agctgcacta
     4081 ccgctccttc gccggcgtcg acccgggctg ggagtgggac cccgggctgc tcgccgcgcg
     4141 ggcggtgctg gagcggcagt tcgacgcggc tctggctgcc gcggtgccga tcgaaccggt
     4201 ggaccccgac ggcgtcggcg atgcgctctt cgagctcgag cgtgccgacg acggtccgtc
     4261 gctctcccgt catctggaga cggtggccga cttcgagcag ttccgtgagt tcgtcgtcca
     4321 ccgctcgctc taccagctga aagagtcaga cccgcactct tgggcgatcc cgcgcctgcg
     4381 ggggcgccga agaccgcgct gctcgaggtc cagcacgacg agtacggcgg cggtcgagca
     4441 gagcgcatgc actccgcgct cttcgccaag acgatgcgcg ggctggggct cgacgacgcc
     4501 gagaacgcgt acttggagcg cgtccccggg gtgacgatcg cgaccgtcaa cctgatgtcc
     4561 cgcttcggtc tgcacgcctc ccggcgcggc gcgatcgtcg gccacctggc gatgttcgag
     4621 atgacctcgg cgcggccgaa ccgccgctac ggcaacgccc ttcgccgcct cggcttcggc
     4681 cccgaggtga ccgacttcta cgacgagcac gtcgaggccg atgccgtgca cgagaacatc
     4741 gccgcttacg acctcgccgg cggcctcgcc cgggccgagc ctgagctggc ctccgacatc
     4801 ctctttggcg ctcgggccct gctctacctc gaagatcgct tcgctcgcca cctgctcggc
     4861 tgctgggagc aagggcggac ctctctgcgg caatcgctgt gaacgccgcc tacgtcctgc
     4921 cgcttcgctg gagcgagccg gggccgctcg ccgagctctc ccggtatctg cgctggctcg
     4981 ccggtgaggt cacagaggtt ctggtgatcg acggctcgcc cgacgacctc ttcgccgcac
     5041 attccctcgc cttcggcagt gacgtccgcc attgccggcc tgaccccgac ctcgattttc
     5101 cgatgggcaa ggtcaacggg gtggtcaccg gcttgcgcgc ctgctcctgc gagcgggtgg
     5161 tgatcgccga cgacgacgtg cgctacgacg gtcagacgtt gcggcggacg ctcgagatgt
     5221 tgactggggc cgatctggtg cgaccgcaga actacttcga cgagctgccc tggcacgctc
     5281 gctgggacag cgcccgctcg ttgctgaacc gggtcttcac cggagacccc gggttcccgg
     5341 tcggggactt ccccggcacg ctggcgctgc gccgctccac cttcttgcgc gccggcgcct
     5401 acgacggcga ggcgctgttc gagaacctcg agctgatgcg gacgatccgc gccgcgggcg
     5461 gcagcgtgct cacgccgctc gacctctacg tcgcccggcg gccgccgggc agcgcccatt
     5521 tccgctccca gcgggtgcgc caggcctacg acgacttcgc gatcccgctg cgcatcggcg
     5581 gctttctcgc gatcggtccg ctgacggggt atttggcgct gcgccggcgc tggtggacgc
     5641 tgggcgcggc tgtgttggcc tcgatcggcg cggccgaggc cggccgccgg cgggcgggag
     5701 ctaccgcgcg tttcccgccc agcggctcgc tgctggcccc tgcctggctc gccgagcggg
     5761 ccgtctgctc ctggctggcg gtgggagcgc ggctgcgcgg gggcgtgcgc tacggcggcg
     5821 gccggctccg tctcgccgcc caccggcagc gggcgctgcg gcggcgctac tccgaatcgg
     5881 gttccggggg accgacgggg ccgggcgaag cctcgctctc ggatcgaaat ccgatcgtct
     5941 tgtgagtccc gtcgcagaag ggccggatct gcgattttcc gcagcggcaa agcgcgatga
     6001 ccggtcgttg gctctcgatc tcgttgccgt cctggtcgca cacgacaaag gagccgcgta
     6061 cgaggtaagg gccgttgcgg tagggcgtga tcttcgtcgg ctcgttcgcc atcctcccgt
     6121 cgctacccgg gacaaacccg tttcatcccg accccgagcg gcgtttccgc cagtgggggc
     6181 cgggtaccgc aaggcttagc cgcgtcgcag cagttgacgt cggcgccgtt cactctcccg
     6241 tggccatacc gggagacaag ggatcggttc agcaagtaca ccgaaccgag aggaaccctc
     6301 cgatgatcat gcgcctcgac cgcctgcagg cggaaatacc accaccgtcg acccctgacc
     6361 ccgtcggcgc ctccgtcgtc caggagctgc tcggcgggaa gttcggcgag atgtcgacct
     6421 tcatgaacta cacgtaccag tcgttcaact tccgcaaccg ccagggcgcg cgccccttct
     6481 acgatctgat cgccagcatc gccgccgagg agttcggtca catcgaactg gtctcgaccg
     6541 cgatcaacac gatgctgacc ggcgccgcga aaggcaaagc cggcaaaaag ggcgcgctca
     6601 aaggcgtcaa ggacacccgc aacacgcagc agttcatcgc cggcggtgcc tcggcgatgg
     6661 tgcaggactc gatgggcaag ccctggagcg gtgactacgt caacgccacc ggcgacctga
     6721 tcggtgacct cacccataat ttctt
//
LOCUS       DASRIG010000428         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3867474, whole genome shotgun sequence.
ACCESSION   DASRIG010000428 DASRIG010000000
VERSION     DASRIG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867474"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFB52_11435"
     CDS             <1..736
                     /locus_tag="VFB52_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO06991.1"
                     /translation="NLIQVQANKYEGLVVWFNQMVESAGGSILKGPQELDLPQEQTER
                     ALEVMGRLSTSEVADPAITTSQEDPNRLAFQTGSSAFMINYPFVYPSAEAEAPDIFKV
                     MKAAKYPAIDPGTPSAPPLGGVNLGVSAFSKNQDLAWKAVECLVGAENQLATAEAGGL
                     PPVREDLYDSKQIEKVYPGFSQIIRESIADAAARPSESPAYQDISLAIQNAVHPTTEI
                     DPEDPSATYDELRELLEQAINREGLL"
     gene            733..1677
                     /locus_tag="VFB52_11440"
     CDS             733..1677
                     /locus_tag="VFB52_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZO06992.1"
                     /translation="MTGAAESVPAQAAPEKTPKISDRTKSERKLAWLLCAPAVIAMLL
                     VTVYPIGYAFVLSLQEVDLRFPDEGGFVGLDNYATVLGSDLWWQGLFNTVFITVVSVA
                     IELVLGMIIALVMYRAIFGRALVRTSVLIPYGIVTVVAAFAWFFAFDPTSGFVNQLPF
                     VADDKAWFGDRWSAMSVIIAAEVWKTTPFIALLLLAGLTTIDEGLYEAAKVDGANAWQ
                     RFWRITLPLMKPAILVALLFRTLDAFRVFDSIFVMTGGGQDTESVSIIGYNQLISRVN
                     LGLGSAVSVLIFVLVLLIAFIYVRGFGTAIPGRERGAN"
     gene            1677..2516
                     /locus_tag="VFB52_11445"
     CDS             1677..2516
                     /locus_tag="VFB52_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZO06993.1"
                     /translation="MKRGTREYTLWSIGLFVILAASLIPVLWIASLSLKAPATIGEGG
                     FFPTEWTFDNYKSLFEGGLDSPLLRPLINSIGIALIATTIAIVLASFTAYAIARLQFP
                     GKLVILGGALAIAMFPPISTVGPLFDMWRNLGLYDTWAGLIIPYLTFALPLAIYTLVA
                     FFREIPFDLEEAAQVDGATPLQAFRKVIVPLAAPGMFTAAILVFIFCWNDFVFAISLT
                     SSDASRTVPAALAFFTGESQFTAPTGNIAAAATLVTIPIIIFVLIFQRRIVAGLTAGA
                     VKG"
     gene            2544..3722
                     /gene="ugpC"
                     /locus_tag="VFB52_11450"
     CDS             2544..3722
                     /gene="ugpC"
                     /locus_tag="VFB52_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZO06994.1"
                     /translation="MADIVLEGVSKRFDDGYEAVKDMDLEVKDGEFVILVGPSGCGKS
                     TALRMIAGLEDITEGELRIGGDVVNQYAPKDRDIAMVFQNYALYPHMTVRDNMAFPLK
                     LAKVPADEIRTKVEEAAKILDLEQHLDRKPANLSGGQRQRVAMGRAIVRDPKAFLMDE
                     PLSNLDAKLRVQMRTEVSRLQKQLSTTTVYVTHDQTEAMTLGDRVAVMRSGVLQQVDT
                     PAVLYSSPINLFVAGFIGSPAMNFMPARLEGDTAKLPIGDVRLPEEARAALGDAGGSR
                     DVIAGARPEAFEDAALVGEAKDHGHTFTAKIDLVESMGSELYAYFPLETEGLQSKELD
                     ELAADSGAAEVPGSGVNQVVARLEPASKVTRGEEAELWLDTSTLLFFDPQSGERLGAQ
                     "
BASE COUNT          592 a         1320 c         1272 g          645 t
ORIGIN      
        1 gaacctgatt caggtgcagg ccaacaagta cgagggcctc gtggtctggt tcaaccagat
       61 ggtcgagtcc gccggcggca gcatcctcaa aggcccgcag gagctcgacc tgccgcagga
      121 gcagaccgag cgggcgctcg aggtgatggg ccggctatcg acatcggagg tcgccgaccc
      181 tgcgatcacg acctctcagg aggaccccaa tcggctcgcg ttccagaccg gtagctccgc
      241 gttcatgatc aattacccgt tcgtctaccc gagcgccgag gccgaggcgc cggacatctt
      301 caaggtgatg aaggcggcga agtacccggc gatcgacccc gggaccccgt cggcgccgcc
      361 gctcggcggc gtcaacctcg gcgtttcggc cttctccaag aaccaggacc tcgcctggaa
      421 ggcggtcgag tgcctggtcg gagccgagaa ccagctcgcg acggcggagg ccggcggcct
      481 acccccggtg cgcgaggacc tctatgactc gaagcagatc gagaaggtct atcccggctt
      541 ctcccagatc atccgtgagt cgatcgccga cgcggccgcg cgaccgtcgg agtcgcccgc
      601 ctatcaggac atctcgctcg cgatccagaa cgccgtgcac ccgacgacgg agatcgaccc
      661 cgaggacccg agcgcgacct acgacgagtt gcgcgagctg ctcgagcagg cgatcaaccg
      721 ggagggtctc ctgtgacagg cgccgcggag tccgtcccgg cgcaggccgc ccccgagaag
      781 acgccgaaga tctcggaccg gacgaagtcc gagcgcaagc tcgcctggct gctgtgcgcg
      841 ccggcggtca tcgcgatgct gctcgtgacg gtctacccga tcggctacgc gttcgtcctc
      901 tcgctgcagg aggtcgacct gcgcttcccc gacgagggtg gcttcgtcgg cctcgacaac
      961 tacgccacgg tgctcggctc ggacctctgg tggcagggac tgttcaacac cgtgttcatc
     1021 accgtggtct cggtcgcgat cgagctcgtg ctcgggatga tcatcgccct cgtcatgtac
     1081 cgggcgatct tcggccgcgc cctggtccgc acctcggtgc tgatcccgta cgggatcgtc
     1141 accgtagtcg cggcgttcgc ctggttcttc gccttcgacc cgacatcggg cttcgtgaac
     1201 cagcttccgt tcgtggccga cgacaaggcc tggttcggcg atcgctggag cgcgatgtcg
     1261 gtgatcatcg ccgccgaggt ctggaagacg actccgttca tcgccctgct gttgctcgcc
     1321 gggctgacga cgatcgatga gggcctctac gaggcggcca aggtcgacgg cgccaacgcc
     1381 tggcagcgct tctggcggat caccctgccg ctcatgaagc cggcgatcct cgtcgcgctg
     1441 ctgttccgga ctctcgacgc cttccgcgtc ttcgactcga tctttgtcat gaccggcggc
     1501 ggccaggaca ccgagtcggt ctcgatcatc ggctacaacc agctgatctc gcgcgtcaac
     1561 ctcggcttag gctcggccgt gtccgtgctg atcttcgtgc tcgtgctgct gatcgcgttc
     1621 atctacgtac gcgggttcgg caccgcgatc ccgggtcgcg agaggggagc gaactgatga
     1681 agcgcggcac gcgcgagtac acgctctgga gcatcggcct gttcgtgatc ctcgcggcct
     1741 cgctgatccc ggtgctgtgg atcgcctcgc tgtcgttgaa ggcgccggcg acgatcggcg
     1801 aaggcgggtt ctttccgacc gagtggacgt tcgacaacta caagagcctg ttcgagggcg
     1861 gcctcgacag cccgctgctg cggccgctga tcaactcgat cgggatcgcc ctgatcgcga
     1921 ccacgatcgc gatcgtcctc gcctcgttca ccgcctacgc gatcgctcgc ctgcagttcc
     1981 cgggcaagct ggtgatcctc ggcggggcgc tggcgatcgc gatgttcccg ccgatctcga
     2041 ccgtcgggcc gttgttcgac atgtggcgca accttgggct ctacgacacg tgggccggcc
     2101 tgatcatccc ctacctgacc ttcgccctgc cgctggcgat ctacaccctg gtcgccttct
     2161 tccgcgagat cccgttcgac ctcgaggagg cggcgcaggt cgacggggcg acgccgttgc
     2221 aggcatttcg caaggtgatc gtgccgctcg cggcgccggg gatgttcacc gccgccatcc
     2281 tcgtcttcat cttctgctgg aacgacttcg tgttcgcgat ctcgctcacc tcctcggacg
     2341 cgtcgcgcac ggtgcccgcg gcgctcgcct tcttcaccgg cgagtcccag ttcaccgctc
     2401 cgaccggcaa catcgccgcc gccgcgaccc tggtcacgat cccgatcatc atcttcgtgc
     2461 tcatcttcca gcggcggatc gtcgccggcc tgaccgccgg agcggtcaag ggatgacccc
     2521 cgacccaaag gagctgcgca gccatggctg acatcgttct cgaaggcgtc tcgaagcgct
     2581 tcgacgacgg ctacgaggcc gtcaaggaca tggacctcga ggtcaaggac ggcgagttcg
     2641 tgatcctggt cgggccgtcc gggtgcggca agtccaccgc gctgcggatg atcgccgggc
     2701 tcgaggacat caccgagggc gagctgcgga tcggcggcga cgtcgtcaac cagtacgcgc
     2761 ccaaggaccg cgacatcgcg atggtgttcc agaactacgc gctctacccg cacatgacgg
     2821 tgcgcgacaa catggccttc ccgctcaagc tggccaaggt tcccgccgac gagatcagga
     2881 ccaaggtcga ggaggcggcg aagatcctgg acctcgagca gcacctcgat cgcaagccgg
     2941 cgaacctctc cggcggtcag cgccagcggg tggcgatggg gcgggcgatc gtccgcgacc
     3001 cgaaggcctt cctgatggac gagccgctgt cgaacctcga cgcgaagctg cgcgtgcaga
     3061 tgcggaccga ggtctcgcga ctgcagaagc agctctcgac gacgacggtc tatgtcaccc
     3121 acgaccagac cgaggcgatg acgctcggcg accgcgtcgc cgtgatgcgg tcaggggtcc
     3181 tccagcaggt cgataccccg gcggtgctct attccagccc gatcaacctg ttcgtcgccg
     3241 ggttcatcgg ctcgccggcg atgaacttca tgccggcgcg gctggagggc gacaccgcga
     3301 agctgccgat cggcgacgtg cgcctgcccg aagaggcgcg cgcggcgctc ggcgacgccg
     3361 gcggctcccg cgacgtgatc gccggcgcgc gcccggaggc gttcgaggac gccgcgctgg
     3421 tgggcgaggc caaggaccac ggccacacgt tcacggcgaa gatcgacctg gtcgagtcga
     3481 tgggctccga gctctacgcc tacttcccgc tcgagaccga gggtttgcag tccaaggagc
     3541 tcgacgagct cgccgccgac tccggcgccg ccgaggtccc gggctcgggc gtcaaccagg
     3601 tcgtcgcccg cctcgagccg gcgagcaagg tcacccgcgg cgaggaggcg gagctgtggc
     3661 tcgacacctc gacgctgctg ttcttcgacc cgcagagcgg cgagcggctc ggggcccagt
     3721 agcggctccg cgagggttcg ccggagtagg tagggcctca ggccgggacc ggcggcggtt
     3781 tgtcctcggc ggggggctcc tcgggctcga ggtcgaggtc gaggtcgag
//
LOCUS       DASRIG010000429         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3307790, whole genome shotgun sequence.
ACCESSION   DASRIG010000429 DASRIG010000000
VERSION     DASRIG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307790"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1048
                     /locus_tag="VFB52_11455"
     CDS             119..1048
                     /locus_tag="VFB52_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO06995.1"
                     /translation="MRAAACLAVLAGTLALAAPAGAADRNHYVVPAGHMTRFALSGTH
                     GYRISVSVNRRNLVTLRTSKGPATTEYVVADRSTSKFGAAAKFPRRGTVAFSFTPNGR
                     VRQLAPIPTCGGARETSHEGVARGVIRFRGERGYTTVRTGRVRAEVRSWPRLKCRFIN
                     FRNRGPERVDAYFYALSTGDPFAEFTATRYVEGARPWSRRIEFNAGIANVNKAMAVYR
                     IVSVVADPATFQQPDPEKMPELLTLTPPSPFSGSAKVERTPESTFTWSGSLKARFPGI
                     DPVKLTSPSFATSFCAGRGCASQVPDDFFDFGR"
     gene            complement(1049..2386)
                     /locus_tag="VFB52_11460"
     CDS             complement(1049..2386)
                     /locus_tag="VFB52_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO06996.1"
                     /translation="MRSRSALPPGPQLPAALQIVGFWTRPLAFLEQCRERYGKRFTIQ
                     LPASPPFVMLTEPDEVKQVFQAPPDVLRPGEGARVLEPIIGSNSVILLDEGAHMEQRK
                     LMLPAFHGERMERLEGLLEEVAVEEVAALPRDVPVSLHPYMQDLTLKVILRAVFGLEP
                     GPRFEALRLRLQEMLVFGDRPISLVPPKPGSLTYRAAERVGPFVRFVRTQEEVDEILF
                     ALIAERRAEHAGDDVLAMLVEARHEDGSPMSDQEIRDELLTLLVAGHETTASSLAWAF
                     SRLVHEPRVLARLVEEVDAGEEAYVTATIQETMRARPVLPNVAPRLVAKPIEVGGWDY
                     EPGCSLVCNGYLIHHDPEIYPDPYSFRPERFLDNPPGTYTWIPFGGGRRRCLGASFAM
                     LEMKIVMRALLGACDLSAAGDAVEVAQRRNITVRPAGGAEVKLAQRERVAALA"
BASE COUNT          411 a          923 c          850 g          390 t
ORIGIN      
        1 gcttctactc cagcctctgc gtaatgagcc cgctgaagaa cgccaacggg tgtgacttcg
       61 ccgaagaaaa cttcccgatg ttccccgaac gactcggcct cttcccgagg tgggcgcagt
      121 gagggccgcc gcctgcctcg cggtgctggc cggaactctg gcgctcgctg cgccggccgg
      181 cgccgccgat cgcaaccact acgtggtccc ggccgggcac atgacccggt tcgcactcag
      241 cggcacccac ggctatcgca tctcggtctc ggtcaaccgg cgaaacctgg tcacgctgag
      301 gacgagcaag ggcccggcga ctaccgaata cgtcgtcgcc gaccgctcga ccagcaagtt
      361 cggcgccgcc gccaagtttc cccgccgtgg cacggtcgcc ttcagtttca cgccgaacgg
      421 gcgcgtgcga cagctcgccc cgatcccgac ctgtggcggg gcgcgggaaa cctcgcacga
      481 gggtgtcgcc cgcggcgtga tccggttccg tggcgagcgc ggctacacga cggtgcgcac
      541 cgggcgcgtg cgcgccgaag tgcgcagctg gccgcggctg aaatgccgct tcatcaactt
      601 caggaatcgc ggccccgaac gcgtggacgc ctacttctac gcgctctcga ccggtgaccc
      661 gttcgcggaa ttcaccgcca cccgctacgt cgagggagcg cgaccgtggt cacgccgaat
      721 cgaattcaac gccgggatcg ccaacgtcaa caaagcgatg gcggtctacc gcattgtctc
      781 cgtcgtcgcc gatcccgcga ccttccagca accggacccg gagaagatgc ccgaactcct
      841 cacgctgacg cccccgagcc cgttcagcgg gagcgccaag gtcgagcgca cgcccgaatc
      901 gaccttcacc tggagcggct cgctgaaggc ccgcttcccc ggtatcgacc cggtgaagct
      961 gacctccccg agcttcgcga ccagcttctg cgcgggtcgc ggctgtgcct cgcaggtccc
     1021 ggacgacttc ttcgacttcg gccgctagct aggcgagcgc ggcgacgcgc tcacgctggg
     1081 ctagcttcac ttccgcccca ccggcgggcc gcaccgtgat gttgcgccgc tgcgcgacct
     1141 cgaccgcgtc gccggctgca ctcagatcgc aggccccgag cagggcgcgc atcacgatct
     1201 tcatctccag catcgcgaag ctggcgccga gacagcggcg gcgaccgccg ccgaacggga
     1261 tccaggtgta ggtgccgggc gggttgtcga gaaagcgctc gggtcggaag gagtacgggt
     1321 cgggatagat ctccgggtcg tggtggatca ggtagccgtt gcagaccagg ctgcagcccg
     1381 gctcgtagtc ccagccgccg acctcgatcg gcttcgcgac cagccgcggc gccacgttcg
     1441 gcagcaccgg gcgggcgcgc atcgtctctt gaatcgtcgc ggtgacgtat gcctcctcgc
     1501 cggcgtcgac ctcctcgacc agccgggcca gcacccgcgg ctcgtgcacc agccgggaga
     1561 aggcccaggc gagagacgag gcggtcgtct cgtggccggc gaccagcagg gtgagcagct
     1621 cgtcgcggat ctcctggtcg ctcatcggcg agccgtcctc gtggcgcgcc tcgaccagca
     1681 tcgccagcac gtcatcgccc gcgtgctccg cccggcgctc ggcgatcagg gcgaagagga
     1741 tctcgtcgac ctcttcctgc gtccgcacga agcggacgaa ggggccgacc cgttcggcgg
     1801 cacggtaggt caggctgccc ggcttcggcg gcaccaggct gatcgggcgg tcgccgaaga
     1861 ccagcatctc ctgcaggcgc aggcgcagcg cctcgaagcg cggaccgggt tcgaggccga
     1921 agacagcgcg caggatcacc ttcagggtga ggtcctgcat ataggggtgc agcgacaccg
     1981 gcacatcgcg gggcagcgcc gcgacctcct cgaccgcaac ctcctccagc aggccctcga
     2041 gccgctccat gcgctcgccg tggaaggccg gcagcatcag cttgcgctgc tccatgtgcg
     2101 cgccttcgtc gagcaggatc accgagttgc tgccgatgat cggctccagg acccgcgcgc
     2161 cctcgccggg ccgcaacacg tccgggggcg cctggaagac ctgcttgacc tcgtcgggct
     2221 cggtcagcat cacgaacggc ggcgaggccg gcagctggat cgtgaagcgc ttgccgtagc
     2281 gctcccggca ctgctcgagg aaggccagcg gccgggtcca gaagccgacg atctgcaacg
     2341 cagcgggaag ctgcggtccg ggcggtagag cggaacgtga gcgcatcact gcctcctcct
     2401 agacgggcgc gtacccattc ataggcctgg cgcccccgcc gcggccgtgc gtttgctcac
     2461 acagtagtaa tcatgtatgg aatgttctgt cttcatttct ctccatcttt cccagcgcaa
     2521 tgaacggaac gcgccacgcc aaacagggtg cagcggggga ggcttcggcg atcg
//
LOCUS       DASRIG010000430         6520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1251005, whole genome shotgun sequence.
ACCESSION   DASRIG010000430 DASRIG010000000
VERSION     DASRIG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251005"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="cysT"
                     /locus_tag="VFB52_11465"
     CDS             complement(<1..796)
                     /gene="cysT"
                     /locus_tag="VFB52_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZO06997.1"
                     /translation="MVLIPLAALVDESLSSGLSHFWDQVTSPQAIRSLELTLIASLIV
                     VAINAVFGTIIAWILVRDNFPGKGAVNAIIDLPFALPTIVASLTLLALYGNDSPFGID
                     IAFSRAAVVVALLFVTLPFVVRAVQPLLIEMDKEMEEAAASLGAGGFTVFRRIIFPNL
                     LPGLAAGVALAFARALGEFGSVLIFAGGLPDTQVSSVFIRTQVESGNLPGASAVSMVL
                     LLASLLCLASISLIQRWGSRHERSTGGALVMPMAGEMPSDAEEKGGD"
     gene            complement(914..1930)
                     /locus_tag="VFB52_11470"
     CDS             complement(914..1930)
                     /locus_tag="VFB52_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZO06998.1"
                     /translation="MLIALFAVGCGGGDDSSSSPDTGGGDTGSSSGSSGGKLDVVGYS
                     TPEYVYTEFMEPAFEETSEGADVSFSNSFGASGDQSRAVVAGQPASIVHFAQGGDMER
                     LVEEGELVSKDWNKNKYKGIAQNSVVVISVRSGNPEGIKSLDDVLSKDVDVVTPNPFS
                     SGAARWNIMAVYGTLINEGKSPDEALAGVKTLLEKTVAQPGSARDALATFTQGEGDVL
                     LGYENEAIKAIEEGEDIEYVVPPSTIQIETPIAVTTDAPEPAAENFLNFIWSDEGQEI
                     WAENGYRPVNPALVDEKQFPTPADLFTIDQFGGWGKVNDEFFDDETGSVAKIETELGV
                     STSG"
     gene            2144..3025
                     /locus_tag="VFB52_11475"
     CDS             2144..3025
                     /locus_tag="VFB52_11475"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HZO06999.1"
                     /translation="MSVDAVRTPDDLLEGLPGYPWQSRYREVDGLRLAHLDEGEGKPV
                     IFFHGEPTWSYLWRKVIPPVRDAGYRCIAPDYAGFGRSDKPTDVGWYTYDRHVELTAA
                     LLEELDLRDATAVVHDWGGPIGLRLAVEHPDRISRLVIMETGPFTGHQKMSEAWLAFR
                     EFVRGSDEVPIGMLVRGGCKNDPGDEVIAAYEAPYIDGPSRAGARAFPLILPTDPEMP
                     GAAEGKAVADALREDERPALVLWADSDPVLPFSVGERVSSQLNFPAPRPIADASHFLQ
                     EDKGEEIGAIVAEWLRA"
     gene            complement(3015..4124)
                     /locus_tag="VFB52_11480"
     CDS             complement(3015..4124)
                     /locus_tag="VFB52_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZO07000.1"
                     /translation="MRTAVISDLHLSLTSGGDVLRDPGVRALLLEEIGEADRVILLGD
                     VIELRERPLGSALAAARPFFEALGAALGEREVVLVPGNHDHRFAEPLLDELSLSATPL
                     GLEHFADPSPGPTELLDRWLGPARLRIAYPGIWVREDVYATHGHYMDQHLKLPRAECI
                     ATATIARIRGPLPAEATAADYERLLRPIYGLAYATAQGRRRPAGHQQKAAETAWEALA
                     GIEKRRSRAAQLRVAAVRTAFPYGIDALNRLLHSDFEPDVSGAAIFRGGLSAATELAT
                     RLGVGDAHVITGHSHRGGPTDEEAEWPLAGGGRLHNTGSWVFSSVFHLPGAPPNAYWP
                     GTVTWVEGEAPPRRLQLLLDRSHGDLLELIATLKP"
     gene            4185..5090
                     /locus_tag="VFB52_11485"
     CDS             4185..5090
                     /locus_tag="VFB52_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO07001.1"
                     /translation="MRGFGEEKTAPANGLEIVYQEMGDPDGEPLFLVMGLATQMIAWH
                     PDFCGLLVDRGFRVVRFDNRDIGRSTKLKAAGLPSRVDMLAGRRATAPYLLSDMARDT
                     FGLMDHLEIGSAHVAGASMGGMIAQTMAINQPARVRSLTSIMSTTGRRFTGMPTLKAF
                     GVLFGGPPKGRDQVIERAVKTFKVIGSPAYPFEEDRIREIAATSYDRGHSAAGVARQL
                     HAILASGDRTTALHDLVVPTSVIHGSADPLIRPSGGRATAAAVPDARLKIIEGMGHDL
                     PQALWTEIADEIAANAARAGDPVAA"
     gene            complement(5159..5242)
                     /locus_tag="VFB52_11490"
     tRNA            complement(5159..5242)
                     /locus_tag="VFB52_11490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5206..5208),aa:Leu,seq:cag)
     gene            5336..5408
                     /locus_tag="VFB52_11495"
     tRNA            5336..5408
                     /locus_tag="VFB52_11495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5370..5372,aa:Glu,seq:ctc)
     gene            5511..>6520
                     /locus_tag="VFB52_11500"
     CDS             5511..>6520
                     /locus_tag="VFB52_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZO07002.1"
                     /translation="MGPGVGEAEIGAVERREGELAWEWGLRLERMGDVGAATAAYAAA
                     VDAGEEPREPRAVLRNAEQLEARGYHEGAEVLFRRAAEAGEPDVRAGAWRGIASYLIG
                     RGEIAAGLEALEAVVATGDPDEAPRALRNIGTLREDYLGEVDGARAAYEAAIAFGHPL
                     HSQGARVNLAQLLDKQGDSAAAAVLFREVIESGHPVECGRARTLLAYLLQEQGDGQQA
                     LEWFEVEMAAERDSEWGQRAAFGAGGIYLDRGDRDRAVEAFRIAERIDNPSEAAMATF
                     FRGEVERERGNQDVALDAYRRAAEADGVVRCAAAKQAGTILLERNDPAAARGLLVLAA
                     EA"
BASE COUNT         1000 a         2194 c         2353 g          973 t
ORIGIN      
        1 cgtcgccgcc tttctcctcg gcgtcgctgg gcatctcgcc ggccatcggc atcaccaggg
       61 cgcctccggt cgagcgttcg tggcggctgc cccagcgctg gatcagcgag atcgaggcca
      121 ggcagagcag cgaggcgagc agcaggacca tcgatacggc cgaggctccg ggcaggttgc
      181 cggactcgac ctgggtcctg atgaagaccg aggagacctg cgtgtcgggc aggccgccgg
      241 cgaagatcag gaccgacccg aactcgccca gcgcgcgggc gaaggccagc gcgacgccgg
      301 ccgcgaggcc ggggaggagg ttggggaaga tgatccggcg gaagacggtg aagccgccgg
      361 cgccgagcga ggcagccgcc tcctccatct ccttgtccat ctcgatcagc agcggctgga
      421 ccgcgcggac gacgaagggc agggtgacga agagcagggc gacgacgacg gcggcgcggc
      481 tgaaggcgat gtcgatgccg aacgggctgt cgttgccgta gagggcgagc agcgtgaggc
      541 tcgccacgat cgtcggcagg gcgaagggga ggtcgatgat cgcgttgacc gctcccttgc
      601 cggggaagtt gtcgcggacc aggatccagg cgatgatcgt gccgaagacc gcgttgatcg
      661 cgaccacgat cagcgaggcg atcagggtca gctcgagcga gcggatcgcc tgcggtgagg
      721 tgacctgatc ccagaagtgg ctgaggccgg agctgagcga ttcgtcgacc agcgcggcca
      781 gcgggatcag gaccatcaag ctcagataga ggactacgta gcctcgaccg aggccaacgc
      841 caagcccggg caggcgcatg cctgcccggg ccttggacgg agtggggagg gctgccgggt
      901 tagcctccat gactcagccg ctggtggaaa cgccgagttc ggtctcgatt ttcgcgacgg
      961 aaccagtttc gtcgtcgaag aactcgtcgt tgactttgcc ccagccaccg aactgatcga
     1021 tcgtgaacag atcggccggg gtcgggaact gtttctcgtc gaccagtgcc gggttgaccg
     1081 ggcggtagcc gttctcggcc cagatttcct gaccctcatc agaccagatg aagttgagga
     1141 agttttcggc cgcgggctcc ggggcgtcgg tggtgacggc gatcggggtt tcgatctgga
     1201 tcgtcgacgg cgggacgacg tattcgatgt cctcgccctc ttcgatcgct ttgatcgcct
     1261 cgttctcgta accgagcaga acgtcgccct cgccctgggt gaaggtggcg agcgcgtcgc
     1321 gggcactgcc cggctgcgcg accgtcttct ccaacagggt tttgacgccg gcgagtgcct
     1381 cgtcggggga tttgccctcg ttgatcaggg tcccgtagac cgccatgatg ttccagcgcg
     1441 cggcgccgga gctgaacggg ttcggggtga ccacgtcgac gtctttgctc agcacgtcgt
     1501 cgaggctttt gatcccctcg gggttgccgc tgcgaaccga gatcacgacg accgagttct
     1561 gggcgatccc tttgtatttg tttttgttcc agtctttgga gaccagttcg ccttcttcga
     1621 cgagccgttc catgtcgcca ccttgggcga agtggacgat cgaggcgggc tgacccgcga
     1681 caaccgctcg gctctggtcg cccgaggcgc cgaacgagtt gctgaaggag acgtcagcac
     1741 cctccgaggt ttcctcgaag gcgggctcca tgaattccgt gtagacgtat tccggggtgg
     1801 aatagccgac cacgtcgagc ttgcctccgc tgcttcctga gctggagccg gtgtcgccgc
     1861 ctccggtgtc agggcttgaa ctgctgtcgt cgcctccacc gcaaccgacg gcgaacaggg
     1921 caatgagcaa cgtgagaaga gcagcgccga agcctgcctt cacgctgcgc cgccgggtat
     1981 tgcgcgcgtt ccgcatctag gatccttcct ttgttgactg acttactact aaatggaagg
     2041 acggcaggct agcacaaagt cgatcaggaa agtatggaat cccgatccgg attcagggaa
     2101 ctaagctccg gcgcgggaga aggtcacaac gggaggagcg gggatgagcg tcgacgccgt
     2161 acgcacgccg gacgatttgc tggaggggct gccgggctat ccgtggcagt cgcgctaccg
     2221 ggaggtcgac gggctgcggc tcgcgcacct cgacgagggc gaaggcaagc cggtgatctt
     2281 cttccacggc gagccgacct ggtcgtacct ctggcgcaag gtgatcccgc cggtccgcga
     2341 cgccggctac cgctgcatcg ctcccgacta cgccggcttc ggccgctccg acaagcccac
     2401 cgacgtcggc tggtacacct acgaccgcca cgtcgagctg accgcggcgc tgctcgagga
     2461 actcgacctg cgcgacgcga ccgcggtcgt ccacgactgg ggcggaccga tcgggctgcg
     2521 cctggcggtc gaacaccccg accggatcag ccgcctggtg atcatggaga ccggtccgtt
     2581 caccggccac cagaagatga gcgaggcctg gctggccttc cgcgagttcg tgcgcggcag
     2641 cgacgaggtg ccgatcggga tgctggtccg cggcggctgc aagaacgatc cgggcgacga
     2701 ggtgatcgcc gcctacgagg ctccttacat agacggacct tcgcgcgcgg gcgcgcgggc
     2761 cttcccgctg atcctgccga ccgacccgga gatgcccggg gccgccgagg gcaaagcggt
     2821 cgccgacgcg ctgcgcgagg acgagcgccc ggcgctggtg ctgtgggccg actccgaccc
     2881 ggtgctgccg ttcagcgtcg gcgagcgcgt ctccagccag ctcaacttcc cggcgccgcg
     2941 gccgatcgcc gacgcctcgc acttcctcca ggaggacaag ggcgaggaga tcggcgcgat
     3001 cgtcgccgag tggctcaggg cttgagcgtc gcgatcagct cgagcaggtc gccgtgcgag
     3061 cggtcgagca gtagctgcag gcggcgcggc ggggcttcgc cttcgaccca ggtgacggtg
     3121 ccgggccagt aggcgttggg cggtgcgccc ggcaggtgga agaccgagga gaagacccag
     3181 ctgccggtgt tgtgcagacg gccgccgccg gccagcggcc actcggcttc ctcgtcggtc
     3241 ggcccgccgc ggtgtgagtg gccggtgatc acgtgggcgt cgccgacgcc gagccgggtc
     3301 gccagctcgg tcgcggcact gagcccgccg cggaagatcg cggcgccgct gacgtcgggc
     3361 tcgaagtcgg agtggagcag gcggttgagg gcgtcgattc cgtacgggaa ggcggtgcgg
     3421 accgcggcga cgcggagctg ggcggcgcgc gagcgccgct tctcgatccc ggccagcgcc
     3481 tcccaggcag tctccgccgc cttctgttgg tggccggcgg gacggcggcg cccctgcgcg
     3541 gttgcgtagg cgagtccata tatagggcgc agcaggcgct cgtagtcggc agccgtggcc
     3601 tcggcgggaa ggggaccgcg gatgcgggcg atggttgccg tggcgatgca ctcggcgcgg
     3661 ggcagcttca ggtgctggtc catgtaatgg ccgtgggtgg cgtagacgtc ctcgcgcacc
     3721 cagatccccg ggtaggcgat gcgcaggcgg gcgggaccga gccagcggtc gagcaattcg
     3781 gtcgggccgg gcgaggggtc ggcgaagtgc tcgagcccga gcggggtggc ggagagcgag
     3841 agctcgtcga gcagcggctc ggcgaagcgg tgatcgtggt tgccggggac gagcacgacc
     3901 tcgcgctcgc cgagcgcggc gccgagcgcc tcgaagaacg gccgggcggc ggcgagggcg
     3961 ctgccgagcg gccgctcgcg cagctcgatc acgtcgccga gcaggatcac ccggtcggcc
     4021 tcgccgatct cctcgagcag cagcgcacgc acccccgggt cgcgcagcac gtcgccgccg
     4081 ctggtcaggc tcaggtgcag gtcggagatg accgcggtcc gcatccgagg gttctacctc
     4141 accgggcgaa ctggctgcgt agccagtttc ggtacgattc ccggatgcgc ggcttcggtg
     4201 aagagaagac ggcacccgcc aacgggctcg agatcgtcta tcaggagatg ggcgacccgg
     4261 acggcgagcc gctcttcctg gtgatgggcc tggcgaccca gatgatcgcc tggcaccccg
     4321 acttctgcgg cctcctggtc gatcgcggct tccgcgtcgt ccgcttcgac aaccgcgaca
     4381 tcggccgctc gaccaagctc aaggcggcgg gactgccgag ccgggtcgac atgctggccg
     4441 gccgccgcgc gaccgcgccc tacctgctct ccgacatggc ccgcgacacc ttcgggctga
     4501 tggaccacct ggagatcggc tccgcccacg tggcgggggc ctcgatgggc gggatgatcg
     4561 cccagacgat ggcgatcaac cagccggccc gggtgcgctc gctgacctcg atcatgtcga
     4621 cgaccggccg ccgcttcacc ggcatgccga ccctgaaagc cttcggcgtc ctcttcggcg
     4681 gcccgcccaa gggccgcgac caggtgatcg agcgcgccgt caagaccttc aaagtcatcg
     4741 gctcgcccgc ctaccccttc gaagaggacc ggatccgcga gatcgccgcc acctcctacg
     4801 accgcggcca ctccgccgcc ggcgtcgccc gccagctgca cgcgatcctc gcctccggcg
     4861 accgcaccac ggccctgcac gacctggtcg tgccgacctc cgtgatccac ggcagcgccg
     4921 accccctgat ccgcccctcc ggcggtcgcg ccaccgccgc cgcggtcccc gacgcccgcc
     4981 tcaagatcat cgagggcatg ggccacgacc tcccgcaggc gctctggacc gagatcgccg
     5041 acgagatcgc cgccaacgcg gcccgcgccg gcgaccccgt cgccgcctag ccctcgtcgt
     5101 ccaaccgccc gcataggggc ggaaacacat cgagcgcccc gcagggcgct cgaatcagtg
     5161 cggctgagag gagtcgaacc tccacgggct ctcgcccaca cggacctgaa ccgtgcgcgt
     5221 ctaccagttc cgccacagcc gcgcggaccc ggcaatctac cgcgtggacg cccctcgccg
     5281 accgctgacc ccgcaaagcg ccgcagatcg cattcgctaa aatgcccgtc cccgcgccgc
     5341 tatcgtctag gggactagga cgccggattc tcagtccgga aaccggggtt cgaatccccg
     5401 tagcggtact gcaggcacgc cgcccaccgg gcggcgtttc tttttggccg actcggtggc
     5461 ttgacggttg agttttgtta agcatccagc cgccggccga ggttttctgc ttgggtcccg
     5521 gtgtggggga agcggagatc ggtgcggtcg agcggcgtga gggcgagttg gcctgggagt
     5581 ggggcctgcg cctggagcgc atgggtgacg tgggcgctgc gaccgccgcc tacgctgcgg
     5641 cggtcgacgc cggggaggag ccgcgggagc cgcgcgccgt cctgcgcaac gcggagcagc
     5701 tcgaggcgcg cggttaccac gagggcgccg aggtgctttt caggcgggcg gccgaggccg
     5761 gcgagcccga cgtgcgcgcg ggcgcctggc gcgggattgc cagctacctg atcggccgcg
     5821 gtgagatcgc ggcgggcctc gaggcgctgg aagcggtcgt cgcgaccggc gacccggacg
     5881 aggccccgcg ggcgctccga aacatcggca ccctcaggga ggactacctc ggcgaggtcg
     5941 acggcgcacg cgccgcctac gaggcggcga tcgccttcgg ccatcccctg cattcgcagg
     6001 gcgcccgcgt caacctcgcg cagctattgg acaagcaggg cgactcggcc gccgccgcgg
     6061 tcctcttccg cgaggtgatc gagtccggcc acccggtgga gtgcggccgg gcgcgaacgc
     6121 tgctggccta cctgctgcag gagcagggcg atgggcagca ggcgctcgag tggttcgagg
     6181 tggagatggc ggccgagcgg gactccgagt gggggcagcg cgccgcgttc ggtgccgggg
     6241 ggatctacct ggaccgcggc gaccgcgacc gcgccgtcga ggcgttccgc atcgcggagc
     6301 gcatcgacaa cccctcggaa gccgcgatgg cgaccttctt ccgcggcgag gtcgagcgcg
     6361 agcgcggaaa ccaggacgtc gccctcgacg cgtatcgaag ggcggccgaa gccgatggcg
     6421 tggttcgctg cgcggccgcc aaacaggccg gaaccatcct gctcgaacgc aacgacccgg
     6481 ccgctgcccg cggcttgctg gtcctcgcgg ccgaagcgga
//
LOCUS       DASRIG010000431         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_732345, whole genome shotgun sequence.
ACCESSION   DASRIG010000431 DASRIG010000000
VERSION     DASRIG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732345"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VFB52_11505"
     CDS             <1..1611
                     /locus_tag="VFB52_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO07003.1"
                     /translation="KVTGVRFGSATTGVSFDNAAGIGSATETPPPLISTLSSVGVNGA
                     FAAGGELGSPGTIVQPAPVAPVNLTSPSLSGAGVVGQPLTCAPGSWSGTPAPSFTYTW
                     LRNGQIIGGASLSTYTPATADLGKPIRCRVTATNTAAAVNAPSNTIQISVLRCTGADI
                     SGAGSSLQGLAHTSVWASAFAADVCNEGTTPEVSYDPIGSAAGMAAWNHDGVSGSIDT
                     SLSFIGTDEAPTPAQVANIESAAGGAKVAVIPVAQTSIAVLANPPAGCSVDAITNSNL
                     VGVMEGRIVNWSKVEGVEGSCNAPITRVVRADGSGTTAQFKNYLQRLYAKGLVCTTGS
                     TEGRASWGELANTAWPESCPEKALSPVVRPAGNGGAALVDTVNATTGSIGYAALPDAA
                     AGKEGSTVLLALQNNGQKKAGEANFADPAQGDVANCIAIGYQVPQLKGSLGIDWSGVF
                     GAKPAIGGSSYPLCTLTYALAFSDYSSAGFTEAQQITTYDYLAGYVLTAPGQTAITGS
                     SYAPLPTSAEALFDVLGASRKAAANIAY"
     gene            1714..1809
                     /locus_tag="VFB52_11510"
     tRNA            1714..1809
                     /locus_tag="VFB52_11510"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1770..1772,aa:Glu,seq:ttc)
BASE COUNT          320 a          805 c          669 g          312 t
ORIGIN      
        1 aaggtcaccg gcgtgcgctt cggctccgcc acgaccggcg tcagcttcga caacgccgcg
       61 ggcatcggct ccgcgaccga aaccccaccg ccgctgatct ccacgctcag ctccgtcggc
      121 gtcaacggcg ccttcgccgc cggcggcgaa ctcggttcgc cggggacgat cgtccagccg
      181 gcgcccgtcg ctccggtcaa cctcacttcg cccagcctca gcggtgccgg tgttgtcggc
      241 cagccgttga cctgcgcgcc cggctcctgg tccggtacgc cggcgccgag cttcacctac
      301 acctggctgc gcaacggcca gatcatcggt ggcgccagcc tcagcaccta cacgccggcg
      361 accgccgacc tcggcaagcc gatccgctgc cgggtgactg cgaccaacac ggccgcggcg
      421 gtcaacgcgc cctcgaacac gatccagatc tcggtcctgc gctgcaccgg cgccgatatc
      481 tccggggccg gctcgagcct gcaggggctg gcgcacacca gcgtctgggc ttcggccttc
      541 gccgccgacg tctgcaacga gggcacgacc cctgaggtca gctacgaccc gatcggctcc
      601 gccgccggga tggcggcctg gaaccacgac ggggtgagcg gctcgatcga cacgagcctc
      661 tcgttcatcg gcaccgacga ggcgccgacc ccggcgcagg tcgccaacat cgagagcgcc
      721 gccggcggcg ccaaggttgc cgtgatcccg gtcgcccaga cctcgatcgc cgtgctcgcc
      781 aacccgcccg cgggctgcag cgtcgacgcg atcaccaact ccaacctggt cggcgtgatg
      841 gaaggtcgga tcgtcaactg gtcgaaagtc gaaggggtgg agggcagctg caacgccccg
      901 atcacccgcg tcgtccgtgc cgacggctcc ggcaccaccg cccagttcaa gaactacctg
      961 cagcgcctct acgccaaagg cctggtctgc accaccggct cgaccgaagg cagggcgagc
     1021 tggggcgagc tggcgaacac ggcgtggccg gagagctgcc ccgagaaagc gctcagcccg
     1081 gtggtccgcc cggccggcaa cggcggcgcg gccctggtcg acacggtcaa cgcgaccacc
     1141 ggctcgatcg gctacgcggc cctgcccgac gcggccgccg gcaaagaggg ctcgaccgtc
     1201 ctcctcgccc tccagaacaa cggccagaag aaagccggcg aagccaactt cgccgacccg
     1261 gcccagggcg acgtcgccaa ctgcatcgcg atcggctacc aggtgcccca gctcaaaggc
     1321 tccctcggga tcgactggtc cggcgtcttc ggcgccaagc ccgcgatcgg cggctccagc
     1381 tacccgctct gcaccctcac ctacgcgctc gccttcagcg actactcatc ggcgggcttc
     1441 accgaggccc agcagatcac cacctacgac tacctcgccg gctacgtcct cacggccccc
     1501 ggccagaccg cgatcaccgg ctcctcctac gctcccctgc cgacctccgc cgaagccctc
     1561 ttcgacgtcc tcggcgcatc caggaaggca gccgccaaca tcgcctactg aggggaccta
     1621 cgcctcctcg cccgctcgct tagaatcctc aaactgcgtt gtggttttgg gagggggtcc
     1681 gcagggcacc caaccgaccc ccagccagca caagcgcccg tagctcagtg gtcaacgggt
     1741 cctttcgggg gcccaaagag cgtcggtctt tcaagccgaa ggtcgcgggt tcgactcccg
     1801 tcgggcgcat tcctcgtgtt tccgttctgt gccggggaat gcccggtgca gagccaagtc
     1861 gtcggtcgag ggctggagag gcgttccggc gggcgccggg cgtttccgtg gcgttccggc
     1921 gcggcggtgt ctcatttctg tctcattcgt ggcgttcggc gaggcgcgcc agttcgacca
     1981 tcgtgacgct gagggccgcg gcgatgtcct tcaccgtggc ccaggtgggg ttggcgttgc
     2041 ccgtctcgat cgccgacacg gtccggccgg tgagtcccgc cttctcggcg agcgcttcct
     2101 gagaga
//
LOCUS       DASRIG010000432         7904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_212378, whole genome shotgun sequence.
ACCESSION   DASRIG010000432 DASRIG010000000
VERSION     DASRIG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212378"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..548)
                     /gene="rplL"
                     /locus_tag="VFB52_11515"
     CDS             complement(150..548)
                     /gene="rplL"
                     /locus_tag="VFB52_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZO07004.1"
                     /translation="MAATKISTEDWIEELKSISVLELSERIKALEEEFGVSATAVAAA
                     APAAAAAGGDGGDAGGEEASTVDVVLTDVGGQKVPVIKVVRAATGLGLKEAKALVDGA
                     PGPIKEGVERDEAEKLKGELEEAGATVELK"
     gene            complement(555..1337)
                     /gene="rplJ"
                     /locus_tag="VFB52_11520"
     CDS             complement(555..1337)
                     /gene="rplJ"
                     /locus_tag="VFB52_11520"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZO07005.1"
                     /translation="MNREEKSATIEEIAGQIEASEAIFAVDYRGISVPQAAELRGKLR
                     EADASFRIVKNRLTKLAAEKAGEDRLAELLQGPTALTFVRGDTAQAAKAITTFNKEHE
                     VLTYKGGFMDGTSLDESAFKSIAKLPSRDVLNGQLAGVVASPLTGLVRGLGSMIQGLA
                     LQLGQIAEKGLVTGEAPSAATAGPVDDGDSSSSRDDAAGEESPSSPPAGEAEEAAPSE
                     ETEGGEGPAEEPQAEAEAPAEAEAADDSADSDTSEDSNEEEG"
     gene            complement(1419..2192)
                     /gene="rplA"
                     /locus_tag="VFB52_11525"
     CDS             complement(1419..2192)
                     /gene="rplA"
                     /locus_tag="VFB52_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZO07006.1"
                     /translation="MAHGKRYRQQYDKVDRDRAYPPAEAVALIKETASAKFDETVEVH
                     FLLGVNVRHADEQLRGTLALPHGLGKDVKIAVFAQGQQARDAEAAGADFVGAQDLAEK
                     IQEGWTDFDVAIATPDMMPVVGQLGRILGPQGKMPNPKVGTVTDDVEKAVSDSKSGKV
                     EYRTDRQAIVHMAIGKASFDADKLLDNYSAVLDEIVRAKPASAKGKYIVSATLASTMG
                     PGVRVDTSSKADRDQAATSTAQPVSEETPAEPVEEPAAA"
     gene            complement(2203..2625)
                     /gene="rplK"
                     /locus_tag="VFB52_11530"
     CDS             complement(2203..2625)
                     /gene="rplK"
                     /locus_tag="VFB52_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZO07007.1"
                     /translation="MAKKVLTLIKLQIQAGNANPAPPVGPALGQHGVNIMDFCKAFNA
                     QTQNEGGTIIPVEITVYEDRSFTFITKTPPAAVLIKEAIRIPKGSGEPHVNKVGTISQ
                     DQIREIAERKMPDLNANDIDAASKIIAGTARSMGVDVK"
     gene            complement(2658..3179)
                     /gene="nusG"
                     /locus_tag="VFB52_11535"
     CDS             complement(2658..3179)
                     /gene="nusG"
                     /locus_tag="VFB52_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZO07008.1"
                     /translation="MYRWYVINTYSGHENKVKHNLEHRVQTMSQNRNVRQVVVPTEQV
                     SEMKDGQKVTAEKRTMPGYVLVNMEMTDDAWGLVKNTPGVTGFVGARNKPVPLSKPEV
                     DRLLHRETAERPRTQAQFAIGESVKVISGPLSDFSGEIAEINEDAAKLKVLVSIFGRE
                     TPVEVGYDQVKKI"
     gene            complement(3208..3735)
                     /gene="secE"
                     /locus_tag="VFB52_11540"
     CDS             complement(3208..3735)
                     /gene="secE"
                     /locus_tag="VFB52_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZO07009.1"
                     /translation="MAKSRAQRKAERRAREAEERKRSDGVRESTSRAQHDTQVPVSGD
                     IAEIEAVEAGIAAGAPEADLETPDAPKPSRSERRAEARTQKESEKRQRAEQKRREAQQ
                     LEKKQKQAKAERAQRGGVIGFLLSCWAELKKVQWPDRDTLIQATAVTVIFVAVAAAYL
                     GALDALFNEIVKQIL"
     gene            complement(3742..3906)
                     /gene="rpmG"
                     /locus_tag="VFB52_11545"
     CDS             complement(3742..3906)
                     /gene="rpmG"
                     /locus_tag="VFB52_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZO07010.1"
                     /translation="MARGDVRIAVTLACEDCKRRNYQTNKSRRNSPDRIEMRKFCRWC
                     GHHTAHKETR"
     gene            complement(4000..4464)
                     /locus_tag="VFB52_11550"
     CDS             complement(4000..4464)
                     /locus_tag="VFB52_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07011.1"
                     /translation="MDFAAGKFRVALGLALMALSLALLVPAAEAKTSLSSARALTPEP
                     AAAAASVPQGMSLEFTKKTATVAGPGALVSVRCVGSQATSCVGTLSIEAPGEPPEVAY
                     SIDSGEQRVVVVPLGEQRTIFDGIVSVKTRVVAHTVQAEGASVRTARTLRFK"
     gene            complement(4647..5789)
                     /locus_tag="VFB52_11555"
     CDS             complement(4647..5789)
                     /locus_tag="VFB52_11555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07012.1"
                     /translation="MDFALSDEQNDFVDAIRNFCDRECGTQDQRERLTEGYAERHSFQ
                     IYEQMAELGWLGVTIPEEYGGSGGTMLDACLFMEETSRGLAPIGGYATTLIVAGATNR
                     FGTEEQKQEILGGIARGAVEAIAMTEPEAGSDVGALTTSAEAVEGGFVVNGQKVFCSN
                     AHIADHVLVVCRTTRSENKHAGMTMLWVPQGADGMEVVPIETMGGRETNHLYFTDCEV
                     PADAVLGEVDQAWTQLMAGLNVERLILAATMLGIAQRAFDDALHYVKERRQFGRPIGS
                     FQALQHRLADLATDLEAARLMTRWVATLTDEDPDRMLPQQASMVKLFVTEVAKRTTIE
                     GMQLMGGYGYSSEYDMERLVRAAMVSTIYGGTSEIQRTIIAKTLGL"
     gene            complement(5834..6199)
                     /locus_tag="VFB52_11560"
     CDS             complement(5834..6199)
                     /locus_tag="VFB52_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07013.1"
                     /translation="MGLISGPLGRRAQRHFDGLDLLREERGANSLGVESQGKAQVRGN
                     GNLALTADELLFAQWIPNRVTRIPRSQILEVTTAKSHLGKWIGRPLLKVSWTNERAEP
                     DSIALWVRDLDAWLAALSG"
     gene            6271..6714
                     /locus_tag="VFB52_11565"
     CDS             6271..6714
                     /locus_tag="VFB52_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07014.1"
                     /translation="MGTDREQIIEVAVRYCTAMDTKDWSLLDRCFVADATVLYPGFDE
                     LRGLPALVGFLDGILGPLDATQHIATNFTVEVEGEEASATSYLHAQHVRHDAPGGPNF
                     IFAGTYRDRLKRTEEGWRITRRELTTTWVEGNSRVLDEVSPERKS"
     gene            complement(6719..7084)
                     /gene="pcaC"
                     /locus_tag="VFB52_11570"
     CDS             complement(6719..7084)
                     /gene="pcaC"
                     /locus_tag="VFB52_11570"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HZO07015.1"
                     /translation="MSDHDRGMEVRREVLGEEHVDRAEAGKDEFTADFQDFITRYAWG
                     EIWDRPGLPRRERSVATLAALVALRAENEIAMHVRAALRNGLSAEEIKEVILHTAIYA
                     GLPAANAAFAIAQAAIAES"
     gene            complement(7081..>7904)
                     /gene="pcaD"
                     /locus_tag="VFB52_11575"
     CDS             complement(7081..>7904)
                     /gene="pcaD"
                     /locus_tag="VFB52_11575"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZO07016.1"
                     /translation="PGRASGTGGSPMIAPAARLHSIVEGPPGAPAIVFLGSLGSDLAM
                     WEPQAEALRGEFRVVRLDTRGHGASPAPPGPYSVAGLGGDVLATLDSLGIERASLCGV
                     SLGGSLAIWLAANAPERVERLVVCFSSAYYGGREAWLERAAVVRAEGTDAVADAVLSR
                     WFTPALAARDPEFVARMRAMIATTPAEGYASCCEALAELDLRDELARIEAPTLVISGS
                     EDPATPAEHGERIAAGVPASRFVLVSGAAHLGNLERPDVVTGLIREHLNQRGGPS"
BASE COUNT         1147 a         2583 c         2752 g         1422 t
ORIGIN      
        1 gactcctcgg gcggtcagag ggacgtagat acagcgcaaa tatgggcagg gacactcaaa
       61 aggcgcgcga ccgcaaaaaa taacacacgg cagcacgcgg ccgcgaactt ggctctagga
      121 gtggtatccc gcgcggcggg acggcggctc tatttgagct cgaccgtcgc gccggcctcc
      181 tcgagctcac ctttgagttt ctcggcctcg tcgcgctcga cgccctcttt gatcgggcca
      241 ggagcaccat cgacaagggc cttcgcctct ttcaggccaa gcccggtcgc cgcgcggacg
      301 accttgatca cggggacttt ctggccgccg acgtcggtca gcacgacgtc aaccgtcgag
      361 gcttcctcgc cgccagcgtc gccgccgtcg ccaccggccg cagcggccgc gggcgccgca
      421 gccgcaaccg cggtcgcgga gacgccgaac tcctcctcga gcgctttgat ccgctcggag
      481 agctcgagga cggagatgct tttcagctcc tcgatccagt cctcggtcga aattttggtc
      541 gctgccattt ggtgttaccc ctcttcctcg tttgaatctt ctgaagtgtc tgaatccgct
      601 gagtcgtcgg cggcctcggc ctccgccgga gcctccgcct cagcttgtgg ctcctcagcc
      661 gggccctcgc cgccctcggt ctcctcactg ggagccgcct cctccgcctc acccgcgggt
      721 ggcgacgatg gggactcctc gcccgcagcg tcgtcccgcg aggacgagga gtccccatcg
      781 tcgacaggac ccgcggttgc ggcgctgggg gcctcgccag tgacgagccc tttctcggcg
      841 atctgcccca actgcagggc caatccttgg atcatcgagc cgaggccgcg gaccaggccg
      901 gtcagcgggc tggcgacgac gccggccagc tggccgttga ggacgtcgcg cgaaggcagt
      961 ttggcgatcg atttgaacgc ggactcgtcg agcgaggtcc cgtccatgaa gccgcccttg
     1021 taggtgagca cctcgtgctc tttgttgaag gtggtgatcg ccttcgcggc ctgggcggtg
     1081 tcgccgcgga cgaaggtgag ggcggtcggg ccctgcagca gctcggcgag acgatcctcg
     1141 ccggctttct cggcggcaag tttcgtcagg cggttcttga cgatgcggaa gctggcatcg
     1201 gcctcgcgca gtttgccgcg cagctcggct gcctggggaa cgctgatacc gcggtagtcg
     1261 acggcaaaga tcgcctcaga ggcctcgatc tggccagcga tctcttcgat agttgcggac
     1321 ttttcctcgc ggttcatctt ctctcctaca ccgggcttat gggttgatcc cgccgatggt
     1381 ctggggtgag cggcgacgca gtgtagcgat cgcgcaattt aggcggcggc aggctcctcg
     1441 accggctcgg ccggggtctc ctcggagacc ggttgggcgg tgctggtggc ggcctggtcg
     1501 cggtcggctt tgctgctggt gtcgacgcgg acgccggggc ccatcgtgct cgcgagggtc
     1561 gccgagacga tgtatttgcc cttcgccgaa gcgggcttgg cgcgaacgat ctcgtcgagc
     1621 accgcgctgt agttgtcgag cagtttgtcg gcgtcgaagc tggccttgcc gatcgccatg
     1681 tgcacgatcg cctggcggtc ggtgcggtat tcgactttgc ccgacttgga gtccgagacg
     1741 gccttctcga cgtcgtcggt gacggtgccg actttcggat tcggcatctt gccctgcggg
     1801 ccgaggatgc ggcccagctg accgaccacc ggcatcatgt ccggggtcgc gatcgcgacg
     1861 tcgaagtcgg tccagccctc ctggattttc tcggccaggt cctgggcacc gacgaagtca
     1921 gcgccggcgg cctcagcgtc gcgcgcctgc tgaccctggg cgaagacggc gatcttcacg
     1981 tctttgccga gcccgtgcgg cagcgccagc gtgccgcgca actgctcgtc ggcgtggcgg
     2041 acgttgacgc cgagcaggaa gtgcacctcg acggtctcgt cgaatttggc gctggccgtc
     2101 tctttgatca gcgcgaccgc ctcggcgggc gggtaggcgc ggtcgcggtc gaccttgtcg
     2161 tactgctggc ggtatcgctt gccgtgggcc acgggaaatc tcctatttga cgtcgacgcc
     2221 catcgagcgg gcggtgccgg cgatgatctt cgaggcggcg tcgatgtcgt tggcgttgag
     2281 gtcgggcatc ttgcgttcgg cgatctcgcg gatctgatcc tgggagatcg tcccgacttt
     2341 gttgacgtgc ggctcgccgg aacccttcgg gatccggatc gcttccttga tcaggacggc
     2401 ggccggcggc gtcttggtga tgaaggtgaa cgagcggtcc tcgtagaccg tgatttcgac
     2461 cgggataatc gtcccgcctt cgttctgcgt ctgcgcgttg aacgctttgc agaagtccat
     2521 gatgttgacg ccgtgctggc ccagcgcggg gccgaccggc ggcgccgggt tggcgttgcc
     2581 cgcctgaatc tgcagcttga tgagggtgag gacttttttg gccatgaggg gttggggagg
     2641 gtagcgctct ttgcgagcta gatcttcttg acctggtcgt agccgacctc gaccggggtc
     2701 tcacgaccga agatcgagac aaggaccttg agtttcgccg cgtcctcatt gatctcggcg
     2761 atctcgccgg agaagtcgga gagcgggccg gagatgactt tgaccgattc gccgatcgcg
     2821 aactgggcct gcgtgcgggg acgctcggcg gtctcgcggt gtaggaggcg gtcgacctcg
     2881 ggtttggaga gcggcaccgg cttgttgcgc gcgccgacga aaccggtgac ccccggggtg
     2941 ttcttgacca gcccccaggc gtcgtcggtc atctccatgt tgacgaggac gtagccgggc
     3001 atcgtccgtt tctcggccgt gactttctgc ccgtccttca tctcggagac ctgctcggtc
     3061 gggacgacga cctgccgcac gttgcggttc tggctcatcg tctgaacccg gtgctcgagg
     3121 ttgtgcttga ccttgttctc gtgcccggag taggtgttga tcacgtacca gcgatacaaa
     3181 attgaagcct ttgctctagg tcctcggtca gaggatctgc ttgacgatct cgttgaaaag
     3241 tgcgtcgagg gcgccgaggt aggcggcggc cacagcgacg aagatgacgg tgacggcagt
     3301 tgcctggatc agggtgtcgc gatccggcca ctggactttc ttcagctcgg cccagcagga
     3361 aagcaggaac ccgatcacgc cgccgcgctg ggcgcgttcg gctttcgcct gtttctgttt
     3421 cttttctagc tgctgggcct cgcggcgctt ctgctcggcg cgctggcgtt tctccgattc
     3481 tttctgggtg cgcgcttcgg cacggcgctc ggagcgcgaa ggcttcggcg cgtccggggt
     3541 ctcgaggtcg gcttcggggg cgccggcggc gatcccggcc tctaccgcct cgatctcggc
     3601 gatgtcgccg gagacgggaa cctgggtgtc gtgctgcgcc cgcgaggtgc tctcgcgaac
     3661 gccgtcggag cgtttgcgct cctcagcttc ccgagcccgg cgctcggcct tgcgctgtgc
     3721 gcggcttttt gccatcgggg actaccgcgt ctccttatgg gcggtgtggt gaccgcacca
     3781 acggcagaac ttgcgcatct cgatacggtc gggcgagtta cgccgcgact tgttggtctg
     3841 gtagttccgc cgcttgcagt cttcacaagc cagcgtcacc gcaatgcgga catctccgcg
     3901 agccatcgtt ttccctcttc ctactctgcc gttacaaaga aaaacctcgg gcccggggcg
     3961 cgaggctgaa caaggatagc gggctgccgc agggcgggac tacttgaacc gcagcgtgcg
     4021 cgcggtgcgc accgaggcgc cctcggcctg gaccgtgtgg gcgaccacgc gggtcttcac
     4081 cgagacgatc ccgtcgaaga tcgtgcgctg ttcgccgagc gggacgacga cgacccgctg
     4141 ttcgccgctg tcgatcgagt aggcgacctc cggtggttcg ccgggcgcct cgatcgaaag
     4201 cgtgccgacg caactggtgg cctggctgcc gacgcagcgg acgctgacca gggcgccggg
     4261 gccggcgacc gtcgccgtct tcttggtgaa ttcgaggctc atgccctgcg gaacagacgc
     4321 tgcggcggcg gccggctcag gggtgagcgc ccgtgccgag gacaaggagg tcttcgcctc
     4381 ggcggcgggc accaggaggg caagcgagag cgccatcagg gcgagcccga gagcgacgcg
     4441 gaacttgcct gctgcgaaat ccatggtgca catgagacca cgaatgttcg atgaattggc
     4501 tcatccatac gtccaaacag gcacctgagg gctaaagtcg ttcgcgaagc gtctcccaga
     4561 gccaagaatc cgcgcctggg cggaccattg tcggatcgcc acgaggggcg atttcggctc
     4621 gattcaggcc gcctcgtgcc cccgcgctag aggccgagcg ttttggcgat gatcgtgcgc
     4681 tggatctcgg aggtcccgcc gtagatcgtc gagaccatcg cggcgcgcac cagccgctcc
     4741 atgtcgtact cggaggagta gccgtagccg cccatcagct gcatgccctc gatcgtcgtc
     4801 cgtttggcga cctcggtgac gaacagcttc accatcgagg cctgctgggg cagcatccgg
     4861 tcgggatcct cgtcggtcag agtcgcgacc cagcgggtca tcaggcgggc cgcctcgaga
     4921 tcggtggcga ggtcggcgag gcggtgctgg agcgcctgga aggagccgat cgggcggccg
     4981 aactggcggc gctccttgac gtagtggagg gcgtcgtcga aggcgcgctg ggcgatcccg
     5041 agcatggtcg cggcgaggat caggcgctcg acgttgagcc cggccatcag ctgggtccaa
     5101 gcctggtcga cttcgcccag aactgcgtcg gcggggacct cgcagtcggt gaagtagagg
     5161 tggttggtct cgcgcccgcc catcgtctcg atcgggacca cctccatccc gtcggcacct
     5221 tggggcaccc agagcatggt catcccggcg tgcttgttct cgctgcgggt ggtgcgacag
     5281 acgacgagca cgtggtcggc gatgtgggcg ttggagcaga agaccttctg gccgttgacc
     5341 acgaaaccgc cctcgaccgc ctcggcgctg gtggtcagcg cgccgacgtc ggagccggcc
     5401 tcgggctcgg tcatcgcgat cgcctcgacg gcgccgcggg cgatcccgcc gaggatctcc
     5461 tgcttctgct cctcggtgcc gaagcggttg gtcgcgccgg cgacgatcag ggtcgtcgcg
     5521 taaccgccga tcggggccag cccgcgcgag gtctcctcca tgaacaggca ggcgtcgagc
     5581 atcgtgccgc cggagccgcc gtactcctcg gggatcgtca ccccgagcca gccgagctcg
     5641 gccatctgct cgtagatctg gaagctgtgc cgctcggcgt agccttcggt gaggcgctcg
     5701 cgctggtcct gcgtgccgca ctcgcgatcg cagaagttcc ggatcgcgtc gacgaaatcg
     5761 ttctgctcgt cggagagggc gaagtccacg ggcggcagcc tatataccgg cggcgtacct
     5821 cgtttggtcg cgctcagccg gacagagccg cgagccaggc gtcgaggtcg cggacccaga
     5881 gggcgatcga gtcgggttcg gcgcgttcat tggtccagga gacctttagc agagggcggc
     5941 cgatccactt gccgaggtgg gacttcgcgg tcgtcacctc gaggatctgc gagcgcggga
     6001 tccgcgtgac gcggttgggg atccactggg cgaagagcaa ctcgtccgcg gtcagcgcga
     6061 ggttgccgtt gccgcggacc tgggccttgc cctgcgactc gaccccgagg ctgttcgccc
     6121 cccgctcttc gcggagcagg tcgaggccgt cgaagtgccg ctgcgcacga cgcccgagcg
     6181 ggccgctgat caggcccatt tgtccggccc cgcgtctgct agtaaccgct cacttagcat
     6241 tacgcccgct tgccacgacg aggaggagat atggggaccg accgagagca gatcatcgaa
     6301 gtggcggtgc gctactgcac cgcgatggac accaaagact ggagcttgct cgaccgctgc
     6361 ttcgttgccg acgcgaccgt gctctacccc ggattcgacg agctgcgcgg gcttcctgcg
     6421 ctggtcgggt tcctcgacgg gatcctcggg ccgctcgacg cgacgcagca catcgccacc
     6481 aacttcacgg tcgaggtcga gggcgaggag gcgagcgcca ccagctacct gcatgctcaa
     6541 catgtgcgcc acgacgcgcc cggcgggccg aacttcatct tcgccggcac ctaccgcgat
     6601 cggctcaagc gcaccgagga gggctggcgg atcacccggc gcgagctgac caccacatgg
     6661 gtcgagggca acagccgcgt gctggacgaa gtaagcccgg aacgaaagtc ttagttccct
     6721 agctttcggc gattgccgcc tgggcgatcg cgaaggcagc gttggcggcg gggagacccg
     6781 cgtagattgc cgtgtggagg atcacctcct tgatctcctc ggcgctgagc ccgttgcgca
     6841 gcgcggcgcg cacgtgcatc gcgatctcgt tctcggcgcg cagggcgacg agcgctgcca
     6901 gggtggcgac gctgcgctca cgcctgggta ggccggggcg gtcccagatc tcgccccagg
     6961 cgtagcgagt tatgaagtcc tgaaagtccg cggtgaactc gtccttgccc gcctcagcgc
     7021 ggtcgacgtg ctcctcgccg aggacttctc ggcgcacctc catgccgcgg tcgtggtcac
     7081 tcaactcggg cctcctcgct ggttcaggtg ttcgcggatc aggccggtga cgacgtccgg
     7141 ccgctcgagg ttgccgaggt gggcggcgcc cgagacgagc acgaagcggg aggcagggac
     7201 ccccgccgcg atcctttcgc cgtgctcggc cggcgtcgcc ggatcctcgc tgccggagat
     7261 caccagcgtc ggcgcctcga tccgagcgag ctcgtcgcgc aggtccagct cggcgagcgc
     7321 ctcgcagcag gacgcgtagc cctcggccgg ggtggtcgcg atcatcgccc gcatccgcgc
     7381 cacgaactcg gggtcacggg cggcaagcgc cggcgtgaac caacgactga ggaccgcgtc
     7441 ggccaccgcg tcggtgcctt ccgcgcgaac gaccgcggcg cgttccagcc aggcctcgcg
     7501 accgccgtag tacgcagagc tgaagcagac gacgaggcgc tcgacgcgct cgggggcgtt
     7561 ggcggccagc cagatcgcga gcgagccgcc aagcgagacg ccgcagagcg aggcgcgctc
     7621 gatcccgagc gagtccaggg tcgcgagcac gtcgccgccg agcccggcga ccgagtaggg
     7681 gccgggcggg gccggcgagg cgccgtggcc acgggtatcg aggcggacga cgcggaactc
     7741 gccgcgcagt gcctcggcct gcggctccca catcgccagg tcgctgccga gtgagccgag
     7801 gaagacgatc gccggcgccc cggggggtcc ctcgacgatg gaatgcagac gggccgccgg
     7861 cgcgatcatc gggctcccac ccgttcccga tgcgcggcca gggc
//
LOCUS       DASRIG010000433         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2530719, whole genome shotgun sequence.
ACCESSION   DASRIG010000433 DASRIG010000000
VERSION     DASRIG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530719"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VFB52_11580"
     CDS             <1..1376
                     /locus_tag="VFB52_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZO07017.1"
                     /translation="VGGVKDCYGAIGVIHSLWKPLPGRFKDFVAMPKANMYQALHTTV
                     IGPEGKPLEIQIRTEEMHTLAEYGIAAHVAYKEGGAVDPQREKMTWLRQLVEAEGEQD
                     PAEFLESLKVDLFEDEVFVFTPKGEVKNLSAGSTPLDFAYAVHTDVGHRTVGAKVNGN
                     IVPLHYQLKSGDIVEVLTAKQNRGPSRDWLKLVRTSRARNKIRAFFKEERREDAERKG
                     RESLDEALRTRGIPMQKVAGSALLADVFREMGYKKGTDFYIALGQGKVSTKTAANKVL
                     QRLKLGESVEERPGAAPQRGREDRARRTKDASNYGIAVKGVENVAVRLAKCCRPVPGD
                     EIAGYVSLGRGITIHRGDCKNVKALKRAPERFVEVSWAGENESSYRVELQIDAYDRTR
                     LLEDLSRTFSEAGINIIGATCTTNPPMVKNRFVVEVGDTEQLKSCITRLRNIESVFDA
                     YRVTPAA"
     gene            complement(1395..2375)
                     /locus_tag="VFB52_11585"
     CDS             complement(1395..2375)
                     /locus_tag="VFB52_11585"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZO07018.1"
                     /translation="MNPKLPHRRHRKPAKKRMLIIVNPYATTVSDRLRNLVVYALQGR
                     FDVEAVATEAQNHATEIGRQAIDGGYDVVVAFGGDGTLNEVANGLAGTDLPVAMLPGG
                     STNVVCRTLGIPNDVVDATEHLLSLADDWKPRKIDLGRVDQRRFVFACGVGIDASVVR
                     RVDENPRLKAKAGPYYYSWAGVSSFFRKYLLKAVRVKVEVGAEEAVEGITAIAQNSDP
                     LTYFGNRPIRVCEGIDFDDGTLSLAVLKRAAQRDMPTLIPRLFSETRTAAGHKQVVHF
                     DDVTSATVSSLSVDKDGKPRAFPVQVDGDYIGDRTRVELGVDPGALTIVA"
     gene            complement(2426..3562)
                     /gene="chrA"
                     /locus_tag="VFB52_11590"
     CDS             complement(2426..3562)
                     /gene="chrA"
                     /locus_tag="VFB52_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HZO07019.1"
                     /translation="MGQESSRSIFIRFLKFGALAWGGPAAQIAMIKRECVDEEGWIPE
                     ERFKKTLAVYQILPGPEAHELCVYFGRERGGKLGGFAAGLGFMLPGFVLMLALSVLYV
                     EADLAGHLDAVFYGLSAAVGAIVLRAVVRLSQTFVTDWWLAAIAIASFALTTWADASF
                     VLVLIGAGIVYELVKNAGRWFGRANSFAPAALVVVVGAVTLSLTAEIFFEGLKAGLLT
                     FGGAFTVIPFLQQGAVVDHGWLTESQFVDGLALSGILPAPLIIFSTFVGYLAGGLAGA
                     LAMTVGIFLPAFVLPIFFHRQLVAIAENDRIHAFLLGVAAGVIGLIAAVTVDIVDTGV
                     SDVWTALLAIAAFAALLRWHGKLTVLYVVLGCGLVGALLQATVV"
     gene            complement(3591..4586)
                     /locus_tag="VFB52_11595"
     CDS             complement(3591..4586)
                     /locus_tag="VFB52_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07020.1"
                     /translation="MTPEEALEQAALAGIHRLSVDTPFRVGPVNCYLVEGDPLTLVDT
                     GPNSGTTLDALQSQLSAHGHAIDDIELIAITHPHLDHLGLTKIIVEHSGAEVAALAPL
                     AEKLKDFPADADEEDAFAVEIMRRNGIPAEVAEALQLVSSSFRGWGSAVDVTRELADG
                     DSIRLGDREFEVQHRPGHSTTDTLFWDADGEILIVGDHLIAHISSNPLISRPLDGSEG
                     RTRSLIEYVESMKRTRELPAKIVLPGHGEPITDHVELIDARLASIQRREEKIYRLIKE
                     QARTGYELAQAIWGNVAVTQAFLTLSEVIGNADVLVEEGRVREADDGTVIRYEAT"
BASE COUNT          777 a         1528 c         1644 g          708 t
ORIGIN      
        1 tcgtcggcgg ggtgaaggac tgctacggcg cgatcggtgt catccactcg ctctggaagc
       61 cgctgcccgg tcgcttcaag gacttcgtcg cgatgccgaa ggccaacatg taccaggcgt
      121 tgcacacgac ggtgatcggg ccggaaggca agcccctgga gatccagatc cgcaccgagg
      181 agatgcacac gctcgccgag tacgggatcg ccgcccacgt cgcctacaaa gagggcggcg
      241 ccgtcgaccc gcagcgggag aagatgacct ggctgcgcca gctggtcgag gccgagggcg
      301 aacaggaccc ggccgagttc ctcgagtcgc tgaaggtcga cctgttcgag gacgaggtct
      361 tcgtcttcac cccgaagggc gaagtgaaga acctctcggc gggctcgact ccgcttgact
      421 tcgcctacgc cgtccacacc gacgtcggcc accgcacggt cggggccaag gtcaacggca
      481 acatcgtgcc gctgcactac cagctgaagt ccggcgacat cgtcgaggtg ctgaccgcga
      541 agcagaaccg tggaccctcg cgcgactggc tgaagctggt gcggacgagc cgcgcccgca
      601 acaagatccg cgccttcttc aaagaggaac ggcgcgagga cgccgagcgc aaggggcgtg
      661 aaagcctcga cgaagcgctg cgcacgcgcg ggatcccgat gcagaaggtc gccggctcag
      721 cgctgctcgc ggacgtcttc cgcgagatgg gctacaaaaa aggcaccgac ttctacatcg
      781 cgctcggcca gggcaaggtc tcgaccaaga ccgccgccaa caaggtcctg cagcggctga
      841 agctgggcga gtcggtcgag gagcggcccg gcgcggcgcc gcagcgcggg cgcgaggacc
      901 gtgctcgccg gaccaaggac gcctccaact acgggatcgc ggtcaagggc gtcgagaacg
      961 tcgccgtgcg tctggccaag tgctgccgcc cggtgccggg cgacgagatc gccggctacg
     1021 tctcgctcgg gcgcgggatc acgatccacc gcggcgactg caagaacgtg aaggcgctca
     1081 aacgcgcccc cgagcgcttc gtcgaggtca gctgggcggg cgaaaacgag tcctcctacc
     1141 gggtcgagct gcagatcgac gcctacgacc gcactcgcct gctcgaggac ctctcgcgga
     1201 ccttctccga ggcggggatc aacatcatcg gcgcgacctg tacgaccaac ccgccgatgg
     1261 tgaaaaaccg cttcgtcgtc gaagtcggcg acacggagca gctcaaatcc tgcatcaccc
     1321 gactgcgcaa catcgagtcc gtctttgatg cgtatcgagt gactccagcg gcgtgacggc
     1381 gccgacgccg ctggtcaggc cacgatagtc agggcgccag ggtccacacc cagctcgacg
     1441 cgggtgcggt cgccgatgta gtcaccgtcc acctggacgg ggaaggcacg cggcttgccg
     1501 tccttgtcga cggagagcga gctgaccgtg gcgctggtca cgtcgtcgaa gtggacgacc
     1561 tgtttgtggc cggcggcggt gcgcgtctcg ctgaacaggc gcgggatcag ggtcggcatg
     1621 tcgcgctgcg cggcccgttt gagcacggcc agcgagagcg tcccgtcgtc gaagtcgatt
     1681 ccctcgcaga cccggatcgg gcggttgccg aagtaggtca gcgggtcgga gttctgggcg
     1741 atcgcggtga tcccctcgac cgcctcctcg gcgccgacct cgactttcac ccgcaccgct
     1801 ttcagcaggt acttgcggaa gaagctcgag accccggccc agctgtagta gtaggggccg
     1861 gctttcgcct tcagtcgggg gttttcgtcg acccgccgca cgaccgaggc gtcgatcccg
     1921 accccgcagg cgaagacgaa gcggcgctgg tcgacccggc cgaggtcgat cttgcgcggt
     1981 ttccagtcgt cggccagcga gagcaggtgc tcggttgcgt cgaccacgtc gttggggatc
     2041 ccgagcgtgc ggcaaacgac gttggtcgag ccgcccggca gcatcgccac cggcaggtca
     2101 gttcccgcga gcccgttggc gacctcgttc agcgtcccgt cgccgccgaa ggcgaccacc
     2161 acgtcgtagc cgccgtcgat cgcctggcgc ccgatctcgg tcgcgtgatt ctgcgcctcg
     2221 gtcgccaccg cctcgacgtc gaagcgcccc tgcagcgcgt agacaaccaa gttgcgcagc
     2281 cggtcggaga ccgtcgttgc gtacgggttg acgatgatca gcatccgctt cttggcgggc
     2341 ttcctgtgcc tgcggtgggg aagtttcgga ttcaccgggg gacgggagcg tattgcgagc
     2401 gccgcgccga cgccgtcgtg agcgcctaga cgacggtcgc ttgcagcagc gcgcccacca
     2461 ggccgcagcc gaggacgacg tagagcacgg tcagtttgcc gtgccagcgc agcagggcgg
     2521 cgaaggcggc gatcgcgagc agggcggtcc agacgtcgct gacgccggtg tcgacgatgt
     2581 cgacggtgac cgcggcgatc aggccgatca cgccggcggc gacgccgagc agaaaggcgt
     2641 ggatgcggtc gttctcggcg atcgcgacga gctggcggtg gaagaagatc ggcaggacga
     2701 aggcggggag gaagatgccg acggtcatcg ccagggcgcc cgcgagcccg ccggccaggt
     2761 agccgacgaa ggtcgagaag atgatcagcg gggcggggag gatgccgctg agcgcgaggc
     2821 cgtcgacgaa ctggctttcg gtcagccagc catggtcgac caccgcgccc tgctggagaa
     2881 aggggatcac ggtgaaggcg ccgccgaagg tgagcaggcc cgccttcagg ccttcgaaga
     2941 agatctccgc ggtgagtgag agggtcaccg cgccgacgac cacgaccagc gccgcggggg
     3001 cgaacgagtt ggcgcggccg aaccagcgac cggcgttctt gaccagctcg tagacgatcc
     3061 cggcgccgat caggacaagc acgaagctgg cgtcggccca ggtcgtgagt gcgaacgagg
     3121 caatcgcgat cgccgccagc caccagtcgg tgacgaaggt ctggctgagc cggacgaccg
     3181 cgcgcaggac gatcgccccg accgcggcgc tgagcccgta gaagaccgcg tcgaggtgcc
     3241 ccgccaggtc ggcctcgacg tagaggaccg agagcgccag catcagcacg aagccgggca
     3301 gcatgaaccc gagccccgcc gcgaagccgc cgagcttgcc gccgcgctcg cgcccgaagt
     3361 agacgcagag ctcgtgcgcc tcgggccccg ggaggatctg gtagacggcg agggttttct
     3421 tgaagcgctc ctcggggatc cagccttctt cgtcgacgca ctcgcgcttg atcatcgcga
     3481 tttgcgccgc cggcccgccc caggcgagcg ctccgaactt caggaagcgg atgaagattg
     3541 agcgcgagct ttcttgaccc atgcgctcaa gatcctaggt tcttctggct ctaggtcgct
     3601 tcgtagcgga tcacggtgcc gtcgtcggct tcgcggacgc ggccttcttc gaccaggacg
     3661 tcggcgttgc cgatcacctc ggagagggtg aggaaggcct gggtgacggc gacgttgccc
     3721 cagatcgcct gcgccagctc gtagccggtc cgcgcctgct ccttgatcag ccggtagatc
     3781 ttctcctcgc ggcgctggat cgaggcgagc cgggcgtcga tcagctcgac gtggtcggtg
     3841 atcggctcgc cgtggccggg caggacgatc ttggccggca gctcgcgggt gcgcttcatt
     3901 gactcgacgt attcgatcag cgagcgggtg cggccctcgg agccgtcgag cgggcggctg
     3961 atcagcgggt tcgaggagat gtgggcgatc aggtggtcgc cgacgatcag gatctcgcca
     4021 tcggcgtccc agaagagcgt gtcggtggtg ctgtgcccgg gccgatgctg gacttcgaac
     4081 tcgcggtcgc cgagacggat gctgtcgccg tcggccagct cgcgggtcac gtcgacggcc
     4141 gagccccagc cgcggaagct gctggagacg agctgcagcg cctcggcgac ttcggctgga
     4201 atcccgttgc ggcgcatgat ctcgaccgcg aaggcgtcct cctcgtcggc gtcggcgggg
     4261 aagtccttca gcttctcggc cagcggcgcg agcgccgcga cctcggcccc ggagtgctcg
     4321 acgatgatct tggtcagccc gaggtggtcg aggtgcgggt gggtgatcgc gatcaactcg
     4381 atgtcgtcga tcgcgtgccc gtgcgcggag agctggctct gcagcgcgtc cagcgtcgtg
     4441 cccgagttcg ggccggtgtc gaccagggtc agcgggtcgc cctcgaccag atagcagttg
     4501 accggcccga cccggaacgg cgtgtccacc gagagccggt ggatgccggc caatgccgcc
     4561 tgctccaatg cttcctcggg ggtcatcgtc acgagcggat cagccccaga acctcgtcgc
     4621 gtttggcctc catgtccgct ttggagatca gcgactc
//
LOCUS       DASRIG010000434         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_734049, whole genome shotgun sequence.
ACCESSION   DASRIG010000434 DASRIG010000000
VERSION     DASRIG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734049"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFB52_11600"
     CDS             complement(<1..866)
                     /locus_tag="VFB52_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZO07021.1"
                     /translation="MTDDERFAIDRDGPHDECGVFGVYAPGHDVSRLAYFGLYALQHR
                     GQESAGIATCEGGHITTLRDAGLVSQVFDEEKLQALQGDMAIGHVRYSTTGGGSWENA
                     QPVWRDDGREVALAHNGNLTNAVELWSELKERGIDFRGTSDSEIIAALLSSHGGREIE
                     DAATDVMPRLQGAYSTVVMTKRAIVAFRDPHGIRPLSLGRIDGKFVVASESCAFDIIG
                     AELMREVHPGEMVSLTARGLETRQVVKAERRATCVFEHIYFSRPDSRLEGKVLQQARG
                     KMGEILWREAPV"
     gene            1028..>2031
                     /locus_tag="VFB52_11605"
     CDS             1028..>2031
                     /locus_tag="VFB52_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07022.1"
                     /translation="MAVSAKGGGWRLATLLALALALLLVPASAAQAVGGSIKGWGQNE
                     LGQVGSGAPGGFYNEPVSVKGIATATAVSSNFDNGMALLADGTVRAWGGGGAGQLGNG
                     DFEDSATPVAVQGLTGVVAIATGRENGVALLADGSVRTWGDNRSGELGIGTTVGPETC
                     PPPAGACSKHPLVVPGLSNVVAIAANDQTVLALLADGSVMAWGGGADGQLGDGSFHPG
                     ACFCEPSPVRVPGVSGAVAVSLGSEFGMALLADGSIRTWGSNDYGQLGIGATSSDLLP
                     AQVPAGVPPAKAIVGGDRQALALLPSGVAYSWGNNYNGELGRGAKTSENCACIPAPGP
                     "
BASE COUNT          287 a          713 c          724 g          307 t
ORIGIN      
        1 tcgaccggcg cctcgcgcca gaggatctcg cccattttgc cgcgcgcctg ctgcagcacc
       61 ttgccctcga gccggctgtc ggggcgggag aagtagatgt gctcgaagac gcaggtcgcg
      121 cgccgctcgg ccttcaccac ctgccgggtc tccaggccgc gcgcggtcag cgagaccatc
      181 tcgcccgggt ggacctcacg catcagttcg gcgccgatga tgtcgaacgc gcagctctcg
      241 gaggcaacca cgaacttgcc gtcgatccgg ccgaggctga gcgggcggat cccgtgcggg
      301 tcgcggaagg cgacgatcgc gcgcttggtc atcaccacgg tcgagtaggc gccctgcaga
      361 cgcggcatca cgtcggtggc cgcgtcctcg atttccctgc cgccgtgcga ggagagcagc
      421 gccgcgatga tctccgagtc ggaggtgccg cggaagtcga tcccgcgctc cttcagctcg
      481 ctccagagct cgaccgcgtt ggtcaggttg ccgttgtggg cgagcgcgac ctcgcggccg
      541 tcgtcgcgcc agaccggctg cgcgttctcc caggagccgc cgccggtggt cgagtagcgc
      601 acgtggccga tcgccatgtc gccctgcagc gcctgcagct tctcctcgtc gaagacctgg
      661 gagacaaggc cggcgtcgcg cagggtggtg atgtggccgc cctcgcaggt ggcgatccca
      721 gccgactcct ggccgcggtg ctggagcgcg tagaggccga agtaggcaag tcgggagacg
      781 tcgtggccgg gtgcgtatac gccgaagacg ccgcattcat cgtgcggtcc atcgcggtcg
      841 atcgcgaacc gctcgtcgtc agtcaaaagc gcgctttcct cgtgggctgg agagacaatt
      901 acgcgcaggc ggactgccta cccgcgcagg gtagcagcga gggtggatcg gcccggtcac
      961 tcgtccgggc gcaatttgcg cggctccgga cttgacagcg ccgactctga tccccaggat
     1021 cccgccgatg gctgtctcgg cgaagggtgg cggttggcgg ttggcgaccc tgctcgccct
     1081 cgccctcgcc ctcctcttgg ttccggcatc cgccgcgcag gcggtcggcg gttcgatcaa
     1141 gggctggggt cagaacgaac tcggtcaggt cggcagcggc gcccccggcg gcttctacaa
     1201 cgaaccggtc tcggtcaaag gcatcgcgac cgcgaccgcg gtctcctcca acttcgacaa
     1261 cgggatggcc ctgctggccg acggcacggt gcgcgcctgg ggtggaggcg gggccggtca
     1321 gctgggcaac ggcgatttcg aagacagcgc cacgccggtt gcggtccagg gcctgaccgg
     1381 cgtggtcgcg atcgcaaccg ggcgtgaaaa cggcgtcgcc ctgctcgccg acggcagcgt
     1441 gaggacctgg ggagacaacc gctccggcga gctcgggatc ggcaccaccg tgggcccgga
     1501 aacctgcccg ccacccgccg gcgcgtgctc gaaacatccg ctggtcgtgc cgggcctgag
     1561 caacgtggtc gcgatcgccg ccaacgacca gaccgtgctg gccctgctcg ccgacggctc
     1621 cgtgatggcc tggggcggcg gcgccgacgg ccagttgggc gacgggagct tccatccggg
     1681 cgcgtgcttc tgcgaaccca gcccggtccg cgtccccggc gtcagcggcg ccgtcgcggt
     1741 ctcgctgggc tccgaattcg ggatggccct gctcgccgac ggctcgatcc ggacctgggg
     1801 ctccaacgat tacgggcagc tcggcatcgg cgccaccagc agcgacctgc tcccggccca
     1861 ggttccggcc ggtgtcccgc ccgccaaagc gatcgtcggc ggcgaccggc aggcgctcgc
     1921 gctgctgccc tccggcgtcg cctactcgtg ggggaataac tacaacgggg agctcgggcg
     1981 gggagcgaag acgagcgaaa actgcgcctg catcccggcc ccagggcctg a
//
LOCUS       DASRIG010000435         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1516395, whole genome shotgun sequence.
ACCESSION   DASRIG010000435 DASRIG010000000
VERSION     DASRIG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516395"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFB52_11610"
     CDS             <1..286
                     /locus_tag="VFB52_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO07023.1"
                     /translation="DPRTLRPLDLDTILESVRKTNRAVVVEEGWPHGGVGANLSTLIT
                     EQAFDHLDAPIQRVTGADVPMPYSKRLEQAAIPHSEHVVKAALATLEGSL"
     gene            286..1614
                     /locus_tag="VFB52_11615"
     CDS             286..1614
                     /locus_tag="VFB52_11615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZO07024.1"
                     /translation="MAEIVMPRLSDSMEEGTILTWMKQVGDEIAVGDEIVEIETDKAN
                     MAYEADVAGTLTEIIAQEGDTLPIGEPIARIGDPGEGPSAPAESSSAGPVGAGDPPSP
                     AVAKASSEMGPPTPPPPDEDSDEAVATDSGGSGDSEAEPGDRIKASPVARRLAKERGV
                     DLSQIEGSGPGGRIVKKDVKKAAKAEDAAPAEEPAAPSTSPSPATTEAPETAKGTVIV
                     EELSKLQQVVARRMSESKATAPHFYLQAEIDMSAAVEGRKQLKELAGEGEGVPSFNDM
                     VVKACALALKEFPRANGSYKDGRFELYSRVNVGVAVAAQDALVVPTVFDADKKGLRQI
                     ASETRALAGRVRDGSITPPELSGGTFTVSNLGMYGISNFSAVINAPQAGILAVGEIAE
                     RPVVRDGELATAHLMGVTLACDHRILYGADGAQFLARIRALLEEPIALAL"
     gene            1742..1948
                     /locus_tag="VFB52_11620"
     CDS             1742..1948
                     /locus_tag="VFB52_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07025.1"
                     /translation="MLAHIAGIPIEETILTFVPLTIAAGSVGFVLLRERARRRSYKRN
                     APEPQRSREPRRAIEPQRAMEPQR"
     gene            1945..2742
                     /locus_tag="VFB52_11625"
     CDS             1945..2742
                     /locus_tag="VFB52_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HZO07026.1"
                     /translation="MSGASPPAAATLAPRALRHRRSARRLTVGQRTAFREAITAAQAL
                     SDDRGYQRWAGVHGLPLPIECVHGGELFLPWHRAYLYFFEKSLQDRVPGVTLPWWDWT
                     NHAEGIPGPYAKSRAEGKKNPLFDSPIQPAARTGSRLTRTVREPGAPERLPSAQEVER
                     VLANRDFMTFQTEVEDLHDGVHGWVGGSMGEIARSAYDPVFWAHHCMIDRLWYLWQLR
                     HPAVGVPASLLNRALAPFPMTVRDTLDITQLGYDYAASTAAVKGPNA"
     gene            2735..3178
                     /locus_tag="VFB52_11630"
     CDS             2735..3178
                     /locus_tag="VFB52_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07027.1"
                     /translation="MRDHFTTRNALELPPADRPFTRADLIFYGIDHSGPSFEALVFMD
                     VRGVGKGADAEHKAYVGSFHIFGHGGCFGDVGHCDVKPKEDPFDLRPAHQLEPRLRIV
                     TVTEPLRELLGRGIEAAKITVIGRSVGKAPNDVLAFDTVRLATYA"
     gene            complement(3180..>3927)
                     /locus_tag="VFB52_11635"
     CDS             complement(3180..>3927)
                     /locus_tag="VFB52_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07028.1"
                     /translation="WFFWIVLAITMVGSLYWQLTASVTQIARSVGDPATTEAYFQPPA
                     EWLGEHGAQSMRIEVPPTANHWEAAYLASKFDLARGWLRQLDTTRDDVFYDSDALNHR
                     SYAAWLRDNAISFVALPDAPLDYSSEVEKRLILSDPGYLTLRHTTEHWRIYAVEDPKP
                     LIAPLGKGRAETLWIGRQGFALDVDRPGRFLVRVNFTPYWSIERGSGCILRQGDWTVA
                     RAAHPGIFRVSADFSLGRAWNAVTGAHKTC"
BASE COUNT          637 a         1338 c         1366 g          586 t
ORIGIN      
        1 cgacccgcgc acgctgcgcc cgctcgacct cgacacgatc ctcgagtcgg tccgcaagac
       61 caaccgcgcg gtggtggtcg aggagggctg gccgcacggc ggggtcggcg ccaacctctc
      121 gaccctgatc accgagcagg ccttcgacca cctcgacgcc ccgatccagc gggtgaccgg
      181 cgccgacgtg ccgatgccct actccaagcg cctcgagcag gcggcgatcc cgcactccga
      241 gcacgtggtc aaggccgcgc tcgccaccct ggaaggatcg ctctaatggc cgagatcgtg
      301 atgccgcggc tctccgactc gatggaggag ggcacgatcc tgacctggat gaagcaggtc
      361 ggcgacgaga tcgcggtcgg cgacgagatc gtcgaaatcg agaccgacaa ggccaacatg
      421 gcctacgagg ccgacgtggc gggcaccctg accgagatca tcgcccagga gggcgacacg
      481 ctcccgatcg gggagccgat cgcgcggatc ggggatccgg gcgagggacc aagcgctccg
      541 gccgaatctt catccgcggg tcctgtcggg gcaggggacc ccccatctcc tgcggtagct
      601 aaagcttcct cggagatggg cccccctacc ccgccacccc cggatgaaga ttcggacgag
      661 gcggtggcga cggactccgg aggctcaggc gatagcgagg cggagccggg cgaccggatc
      721 aaggcgtcgc cagtggcgag gaggctcgcg aaagagcgcg gcgttgacct ctctcagatc
      781 gagggctcgg gaccgggcgg caggatcgtc aagaaggacg tgaagaaggc ggcgaaggct
      841 gaggatgctg cgcctgcgga ggagcccgct gctcccagca cctccccctc ccccgctacg
      901 accgaggctc ccgaaaccgc caagggcacg gtcatcgtcg aggagctttc gaagttgcag
      961 caggttgtcg cgcgccggat gtcggagtcg aaggcgacgg cgccgcactt ctacctgcag
     1021 gcggagatcg acatgtcggc ggcggtcgag gggcgtaagc agctgaagga gttggccggc
     1081 gagggcgagg gtgtgccctc gttcaacgac atggtcgtca aggcctgcgc gctggccctg
     1141 aaggagttcc cgcgcgccaa cggctcttat aaggacggcc gcttcgagct ttactcgcgg
     1201 gtcaacgtcg gggtcgcggt cgccgcccag gacgcgctcg tcgtcccgac cgtgttcgac
     1261 gccgacaaga agggcctgcg gcagatcgcc agcgagacca gggcgctggc cggccgggtg
     1321 cgcgacggct cgatcacccc gcccgagctc tccggtggca ccttcaccgt ctccaacctc
     1381 gggatgtacg ggatctcgaa cttctccgca gtgatcaacg cgccgcaggc cggcatcctc
     1441 gcggtcggcg agatcgccga gcggcctgtc gtccgcgacg gcgagctggc caccgcgcac
     1501 ctgatgggcg tcaccctcgc ttgcgatcac cgcatcctct acggcgccga cggggcccag
     1561 ttcctggccc ggatcagggc cctgctggaa gagccgatcg cgctcgcgct ctaagaagaa
     1621 cctgtgcatt cgtgcgcttt tcggggcttt caccccgata ttcggggtga ccgaatgcgg
     1681 atattcgccc ttactataga aacgtttgtg tagtcagaag acctgtgtaa gtgtgaccgt
     1741 gatgttggcc cacattgcgg gaatcccgat cgaggagacg attttgactt tcgtcccgct
     1801 gacgatcgcg gcgggcagcg tcggcttcgt cctgcttcgc gaacgggcgc ggcgacggag
     1861 ctacaagcgc aacgcgcccg agccgcagcg gtcgagagag ccgcgccgcg cgatcgagcc
     1921 gcagcgcgcg atggaaccgc agcggtgagc ggtgcctctc ctccggccgc cgcaacgctc
     1981 gcgccgcgcg cgttgcgcca tcgccgcagc gcgcggcggt tgacggtcgg ccagcgcacc
     2041 gccttccgcg aagcgatcac cgcagcgcag gcgctcagcg acgaccgcgg ctaccagcgc
     2101 tgggccgggg ttcacggcct gccgctgccg atcgagtgcg tgcacggcgg cgagctcttc
     2161 ctgccctggc accgcgccta cctctacttc ttcgagaagt ccctgcagga ccgagtgccc
     2221 ggcgtcaccc tgccctggtg ggactggacg aaccacgccg agggcattcc cggtccctac
     2281 gcgaaaagcc gcgcggaagg aaagaagaat ccgctcttcg actcgccgat ccagcccgcg
     2341 gcccgcaccg gctcgcgact cacgcggacg gtgcgcgaac cgggtgcgcc ggagcgtctg
     2401 ccgtcagctc aggaggtcga acgggtgctg gccaaccgcg atttcatgac cttccagacc
     2461 gaggtcgaag acctccacga cggggtgcat ggctgggtgg gcggctcgat gggggaaatc
     2521 gcccgctccg cctacgaccc ggtgttctgg gcccaccact gcatgatcga ccgcctctgg
     2581 tacctctggc agctgcgcca ccctgccgtc ggcgtgccgg cctcgctgct caaccgggcg
     2641 ctggcgccgt tcccgatgac ggtccgcgac acgctggaca tcactcagct cggttacgac
     2701 tacgccgcct caaccgcggc cgtgaaaggg cccaatgcgt gaccacttca ctacccggaa
     2761 cgcgctcgag ctgccgcccg ccgaccgtcc gttcacccgc gccgacctga tcttctacgg
     2821 gatcgaccac tccggcccct cgttcgaggc cctggtcttc atggacgtac gcggggtcgg
     2881 caaaggcgcc gacgcagagc acaaggccta cgtcggctcc tttcacatct tcggccatgg
     2941 cggctgcttc ggcgatgtcg gccactgcga cgtcaagccc aaagaggacc ccttcgatct
     3001 gcgtcccgcc caccagctgg aaccgcgact gcggatcgtc accgtgaccg agccgctgcg
     3061 ggagctgctc gggcgcggga tcgaggcggc gaaaatcacc gtgatcggcc gctcggtcgg
     3121 caaagcgccg aacgacgtcc tcgccttcga cacggtccgg ctcgcgacct acgcctgact
     3181 cagcaggtct tgtgggcgcc ggtgacggcg ttccaggccc ggcccagcga gaagtcggcc
     3241 gagacccgga agatgccggg atgggcggcg cgggcgacgg tccagtcgcc ctggcggagg
     3301 atgcagccgc tgccgcgctc gatcgaccag tacggggtga agttgacccg gacgaggaag
     3361 cggccggggc ggtcgacgtc gagggcgaag ccctggcggc cgatccagag cgtctcggcg
     3421 cggcctttgc cgagcggcgc gatcagcggc ttcgggtcct cgaccgcgta gatccgccag
     3481 tgctcggtgg tgtggcgcag ggtgaggtag ccgggatcgc tgaggatcag ccgtttctcg
     3541 acctcggagg agtagtcgag cggcgcgtcg gggagggcga cgaaggagat cgcgttgtcg
     3601 cgcagccagg cggcgtagga gcggtggttg agggcgtcgc tgtcgtagaa gacgtcgtcc
     3661 cgggtggtgt caagctggcg cagccagccg cgcgcgaggt cgaacttcga cgccaggtag
     3721 gcggcctccc agtggttggc ggtcggcggg acctcgatcc gcatgctctg cgcgccgtgt
     3781 tcgcccagcc attcggccgg cggctggaag taggcctcgg tggtcgccgg gtcgccgacg
     3841 ctacgggcga tctgggtgac gctggcggtc agctgccagt agaggctgcc gaccatcgtg
     3901 atcgccagca cgatccagaa gaaccac
//
LOCUS       DASRIG010000436         9472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3069826, whole genome shotgun sequence.
ACCESSION   DASRIG010000436 DASRIG010000000
VERSION     DASRIG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3069826"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFB52_11640"
     CDS             <1..211
                     /locus_tag="VFB52_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07029.1"
                     /translation="EILLKLQEMVAAGTSILFVSSELEEVAGVSDRVLVLDGGRVVAE
                     LQSDEGITPTAILSHVLQVEDPDEQ"
     gene            201..1223
                     /locus_tag="VFB52_11645"
     CDS             201..1223
                     /locus_tag="VFB52_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07030.1"
                     /translation="MSNETRTEPPKLAPAGGPVEQAPARGLRMNRREATDFALRYAMV
                     GVAIVLAIGAEIAYPGFFAWKSIEQMLSQNAGQGLVAMGMTFVIIAAGFDLSVAAVFA
                     LGAVCYVGLGEDMGLGYAFILVILIGVVAGIVNGLIITRLKVNAFVATLGTASIFGGI
                     AYLASNEQTLSSTKAGITHLGLDKWLGMQIPVWILIGAFVIGGVLLAFTVFGRSVYSV
                     GGNREAARLAGIRVDVITLLTYVIVGAMAALAGILSASTTGVAGANEGGEITLAAIAI
                     VIIGGTSLFGGEGAMWRTGVGLAIFAMIDHLFILMNVNTPGQLIAKGAIVIIAVAIDA
                     WSRTRG"
     gene            complement(1220..2152)
                     /locus_tag="VFB52_11650"
     CDS             complement(1220..2152)
                     /locus_tag="VFB52_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO07031.1"
                     /translation="MILRLAHVVIAAEDLAAARAFYVDLLGFSEHLQDGDALYLRAAE
                     GFDTWSLAIVERGSGGLLRSAFRVSDPDDLDRLEQLHERLGTRTQRVPAGTAPGQGPA
                     LRVQTPEGHAIEFVHEVEEIEPYDADGKLRLPMRNTNTAGGIPPARLDHVSLRVPSVP
                     ESLPYWTGELDFSVSEFWMNEDDVPHIAWVRRAANTHDVALGTHGSAAFHHFAYAVSD
                     PHGLLRAADLMGDARIPQQLEWGPARHGATNAFAMYLRDPSGNRLELYSGDYVRDLDR
                     PPLYWRPNSYGLQGHSWWDYPPPPSFAETLPLLD"
     gene            complement(2149..3174)
                     /gene="dmpG"
                     /locus_tag="VFB52_11655"
     CDS             complement(2149..3174)
                     /gene="dmpG"
                     /locus_tag="VFB52_11655"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: protein motif:HMM:NF006049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZO07032.1"
                     /translation="MSAEKLYVQDVTLRDGMHAMRHRIEIDKLTAIVAALEAAGVTAI
                     EVTHGDGLAGASVNYGAGSHTDWEWIEAACAQMERAVPTSLLLPGVGTIADLERAVQL
                     GIRSVRVATHCTEADVSAQHISTARELGLDVAGFLMMAHLSPPAELAEQAKLMESYGA
                     HCVYVTDSAGALTMDGFRERIEAYREALDPGTEVGVHAHQNLSLAVANSVVAVEAGAH
                     RVDASLAGHGAGAGNTPIEAFVAVADKLGWETGCSVFALQDAADDLVRPLQDRPVQLD
                     RETLTIGYAGVYSSFLRHAEDAAETYGVDVRELLTEAGERALIGGQEDMLADIALDRV
                     RAREAAQ"
     gene            complement(3171..4109)
                     /locus_tag="VFB52_11660"
     CDS             complement(3171..4109)
                     /locus_tag="VFB52_11660"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HZO07033.1"
                     /translation="MTATKVAIIGSGNIGTDLMFKVRRSEALEVAAMVGIDPDSDGLA
                     RAGRMGVATTSDGVAGLIEMPEFEAIEIVLDATSAKGHEANAEALAPHGKRLVDLTPA
                     ALGPFVIPVVNLEQHLDAPNVNMVTCGGQATIPIVAAVSRVTPVPYAEIVASVASRGA
                     GPGTRANIDEFTETTAHAIESVGGAARGKAIIILNPAEPPLIMRDTVFCLVGECDHDA
                     VRDSIGRMVADVAHYVPGYRLKQEVQITPVDPASVSTLVDPAEAEVRWKVSTFLEVEG
                     AADYLPAYAGNLDIMTSAALRVAEEIAARPQVGAGR"
     gene            complement(4111..4905)
                     /locus_tag="VFB52_11665"
     CDS             complement(4111..4905)
                     /locus_tag="VFB52_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO07034.1"
                     /translation="MESMQRSIEEAAQRLLGAQESGTPAEPVRELIGATDIDLAYRVQ
                     QQVIAARLAGGAKVVGRKIGLTSKAVQEQVGVDQPDFGFLLDEMSYADGETIPYSSLL
                     QPRAEVEIAFVLAAPIADVADPEEVRAAVDYAVAAIEVVASRVRDWDIKITDTVADNA
                     SSGAFVLGAERVALADFDPPAVTMSLTRAGEEISSGTGAACLGDPLNALAWLAEAAAR
                     YGSPLGAGDVVLSGALGPLAVVFPGDELTASISSLGTVTARFDQGG"
     gene            complement(5040..5729)
                     /locus_tag="VFB52_11670"
     CDS             complement(5040..5729)
                     /locus_tag="VFB52_11670"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07035.1"
                     /translation="MPSRDKGRTPAGSQTLARGLTALHLICDSPGGLTIQEVAEQLEI
                     HRTVAARLLATLEQFHLIARHEGRFRPGAGLAALGASFDQNLREVSMPVLRRLADGTQ
                     ATASLLVAEGEEQVAIAVIVPAGLSYHLSFREGSRYPIDRGAAGLALLASAPPEPEER
                     EVVAEARKQGWVLTHGEVEPNAYGLAVPVKRTPPSPATAINLVSHRPDVLEEARPTVV
                     EAARELAGILG"
     gene            5886..6713
                     /locus_tag="VFB52_11675"
     CDS             5886..6713
                     /locus_tag="VFB52_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07036.1"
                     /translation="MPFEQGYLDVGGCRTRYLHAGAEDQPTLVLLHGSAGHAEAYVRN
                     LDAHAAHFSTWAIDMVGHGFTDKPGHPLEVEHYVEHLLAFLDEIGVERAHLSGESLGG
                     WVVSRAAIDHPERVDRLVLNTAGGSQADPEVMARIRTLSMAAAENPDWETVGARIRWL
                     MADKERNYDDVIASRQAVYRQPGFVGAMDDILALQDPEIRARNIVSPEDYGRIAAPTL
                     VLWTSDDPTADVSEGRRIATMIPGAEFVVMEGCGHWPQYEDPAEFNRIHLDFLRGSA"
     gene            6710..8338
                     /locus_tag="VFB52_11680"
     CDS             6710..8338
                     /locus_tag="VFB52_11680"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZO07037.1"
                     /translation="MRPDVVIVGAGPVGLTLANFLGMHGVRTVVVEQREELIDYPRGV
                     GIDDESFRAFQAIGLIEPILPHTVPNQVMRFVNGKGRVLAEIAPSEQPFGWPRRNGFI
                     QPLVDEQLLAGLDRFECVEVLWERTLESLATTADGAAVTVSGPDGPETIRARYVVGCD
                     GGRSTTRRLTETSFEGTTSATRWVVIDLRNDPLGSPNVTVGADPARPFVSVSLAHGVR
                     RFEFMVHDDESDEQVEDPEFLAELLRPHVPYPSQIDVIRKRVYTHHSRIAGSFRAGPV
                     FIAGDAAHLMPVWQGQGYNSGIRDAVNLGWKLALVIRGAAGDALLDSYDPERRDHARA
                     MIDLSTTVGKWVSPTSTRVAALRDVVLRVISAIPSFKRYLVEMRFKPMPRYARGAITE
                     LHAADDGVVGRQFIQPRVDTRERRGVQLDEVTGPWFSVLCWNNDPRAVLGEEAERWLA
                     LGARLIELRPQSQLHWDGSGREGVEVVGDASGALKKWFDGQRSSVLFLRPDRFVAAAC
                     VAQHAPEVSRALASALSLTEGSAKLEPAAPAARG"
     gene            8344..>9472
                     /locus_tag="VFB52_11685"
     CDS             8344..>9472
                     /locus_tag="VFB52_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO07038.1"
                     /translation="MEPGFDQSVDLLVVGSGGGGLTTAMAAKAAGLETLLVEKSERFG
                     GSTALSGGGIWIPGASAQVRAGYRPDPEETLEYLRTITKGVVSEERLRAYVETGPEML
                     DFIERHTDAELVWKPGYPDYFPEEPGGSAEGSVINLEPIDLRKLGPDEEKLFGPAAAG
                     PKGMWLRPLELKEFFTIRQSWRGKLVLLRFLWRSLRARVTGERVVALGQALVAQLWLG
                     LRRLGVPVWLGSPLVSLITGEDGAVRGAVIRHEGREVRVEARGGVVLATGGFEHNEEM
                     RRQYQPFVPEDFSLGNPASTGDGIRAGQEAGAALDLMDEAWWFPTLAWPSGRLHMMLN
                     ERMMPAQFIVNGAGERFINEATPYSEFGHAMIEGQATGTTYM"
BASE COUNT         1331 a         3268 c         3489 g         1384 t
ORIGIN      
        1 cgagatcctg ctgaagctgc aggagatggt cgcggcgggg acctcgatcc tcttcgtctc
       61 ctccgagctg gaggaggtgg cgggggtcag cgatcgcgtg ctcgtcctcg acgggggccg
      121 ggttgtggcg gaactgcaaa gcgacgaagg gataacgccg acggcgatcc tcagtcacgt
      181 actccaagtg gaggatccag atgagcaatg agacccgcac cgagcccccg aagctggcgc
      241 ccgcaggtgg acccgtcgag caggcgccgg cgcgcggcct gaggatgaac cgccgcgagg
      301 cgaccgactt cgccctgcgc tacgcgatgg tcggggtcgc gatcgtgctc gcgatcggcg
      361 ccgagatcgc ctaccccggc ttcttcgcct ggaaaagcat cgagcagatg ctgagccaga
      421 acgccgggca ggggctggtc gcgatgggga tgaccttcgt gatcatcgcc gccggcttcg
      481 acctctcggt cgccgccgtg ttcgcgctcg gggcggtctg ttacgtcggc ctgggcgagg
      541 acatggggct cgggtacgcc ttcatcctgg tgatcctgat cggggtcgtc gccggaatcg
      601 tcaacggcct gatcatcacc cggctcaaag tcaacgcctt cgtcgccacc ctcggcaccg
      661 cctcgatctt cggcgggatc gcctacttgg cctcgaacga gcagacgtta agttcgacca
      721 aggccgggat cacccacctc ggcttggaca agtggctggg gatgcagatc ccggtctgga
      781 tcctgatcgg cgccttcgtg atcggcggcg tcctgctcgc cttcaccgtc ttcggccgct
      841 cggtctactc agtcggtggc aaccgcgagg cggcccgcct ggccgggatc cgggtcgacg
      901 tgatcaccct gctcacctac gtgatcgtcg gcgcgatggc ggccctggcc gggatcctct
      961 cggcctcgac caccggcgtc gccggcgcca acgaaggcgg cgaaatcacc ctcgcggcga
     1021 tcgcgatcgt gatcatcggc ggcacctccc tgttcggcgg cgagggcgcg atgtggcgca
     1081 ccggggtcgg cctcgcgatc ttcgcgatga tcgaccacct cttcatcctg atgaacgtca
     1141 acacccccgg ccagctgatc gccaagggtg cgatcgtgat catcgccgtg gcgatcgacg
     1201 cctggagccg aacgcgcggc tagtcgagga gcggcagggt ctcggcgaag ctggggggtg
     1261 gggggtagtc ccaccaggag tggccctgga ggccgtagga gttggggcgc cagtagagcg
     1321 gggggcggtc gaggtcgcgg acgtagtcgc cgctgtagag ctcgaggcgg ttgccggagg
     1381 ggtcgcgcag gtacatcgcg aaggcgttgg tggcgccgtg gcgggcgggc ccccactcga
     1441 gctgctgggg gatgcgggcg tcgcccatca ggtcggcggc gcggagcagg ccgtgggggt
     1501 cggagactgc gtaggcgaag tggtggaagg cggccgagcc gtgggtgccg agggcgacgt
     1561 cgtgggtgtt ggcggcgcgc cggacccagg cgatgtgcgg cacgtcgtcc tcgttcatcc
     1621 agaactcgga gacgctgaag tcgagctccc cggtccagta gggcagcgac tcggggacgc
     1681 tggggacgcg cagggagacg tggtcgagcc gggccggggg gatgccgccg gcggtgttcg
     1741 tgttgcgcat cggcaggcgc agcttgccgt cggcgtcgta gggctcgatc tcctcgacct
     1801 cgtggacgaa ctcgatcgcg tggccttcgg gcgtctgcac ccgcagggcc gggccctggc
     1861 cgggcgcggt cccggccggc acccgctggg tacgcgtgcc gagccgctcg tgcagttgct
     1921 cgagccggtc gaggtcgtcg gggtcggaga cgcggaaggc ggagcgcagg agcccgccgg
     1981 agccgcgctc gacgatcgcc agcgaccagg tgtcgaaccc ctcggcggcg cgcaggtaga
     2041 gcgcgtcgcc gtcctgcagg tgctcgctga agccgagcag gtcgacgtag aaggcgcggg
     2101 ccgcggcgag gtcctcggcc gcgatcacga cgtgggcgag gcggaggatc attgcgccgc
     2161 ctcccgcgcc cgcacgcggt cgagggcgat gtcggcgagc atgtcctcct ggccgccgat
     2221 cagggcccgt tcgccggcct cggtgagcag ctcgcggacg tcgacgccgt aggtctcggc
     2281 ggcgtcttcg gcgtggcgca ggaagctcga gtagacgccg gcgtagccga tcgtcagggt
     2341 ctcgcggtcg agctgcaccg gccggtcctg cagcggccgc acaaggtcgt cggcggcgtc
     2401 ctgcagggcg aagaccgagc agccggtctc ccagcccagc ttgtcggcga ccgcgacgaa
     2461 tgcctcgatc ggggtgttac cggcgccggc gccgtggccg gccagcgagg cgtcgacccg
     2521 gtgcgccccg gcttcgaccg cgaccaccga gttggcgacc gcgagcgaga ggttctggtg
     2581 cgcgtgcacg ccgacttcgg tgccggggtc gagcgcctcg cggtaggcct cgatccgctc
     2641 gcggaagccg tccatggtca gcgccccggc cgagtcggtg acgtagacgc agtgggcgcc
     2701 gtaggactcc atcagcttcg cctgctcggc cagctcggcg ggcggcgaga ggtgggccat
     2761 catcaggaag ccggcgacat cgagcccgag ctcgcgcgcg gtcgagatgt gctgggcgga
     2821 gacgtcggcc tcggtgcagt gggtggcgac ccggaccgag cggatgccga gctgcaccgc
     2881 gcgctcgagg tcggcgatcg tgccgacccc gggcaggagc agcgaggtcg ggacggcgcg
     2941 ctccatctgc gcgcaggccg cctcgatcca ttcccagtcg gtgtggctgc cggcgccgta
     3001 gttgacgctc gcccccgcga ggccgtcgcc gtgggtgacc tcgatcgcgg tgacgccggc
     3061 cgcctcgagc gcggcgacga tcgcggtcag cttgtcgatc tcgatgcggt ggcgcatcgc
     3121 gtgcatgccg tcgcgcaggg tgacgtcctg cacgtagagc ttctcggcgc tcatcggccc
     3181 gctccgacct gcgggcgggc ggcgatctcc tcggccaccc gcagcgccgc cgaggtcatg
     3241 atgtcgaggt tgcccgcgta ggccggcagg tagtcggcgg caccctcgac ctcgaggaag
     3301 gtcgagactt tccagcgcac ctccgcctcc gccgggtcga ccagggtgct gaccgaggcg
     3361 gggtcgacgg gggtgatctg cacctcctgc ttcaaccggt agccgggcac gtagtgggcg
     3421 acgtcggcga ccatccggcc gatcgagtcg cggaccgcgt cgtggtcgca ctcgccgacc
     3481 aggcagaaga ccgtgtcgcg catgatcagc ggcggctcgg ccgggttgag gatgatgatc
     3541 gccttgccgc gcgccgcgcc gccgacgctc tcgatcgcgt gcgcggtggt ctcggtgaac
     3601 tcgtcgatgt tggcccgggt gccggggccg gcgccgcgcg aggcgaccga ggcgacgatc
     3661 tccgcgtagg ggaccggggt gacccgggag acggcggcga cgatcgggat cgtcgcctgg
     3721 ccgccgcagg tgaccatgtt cacgttcggc gcgtcgaggt gctgctcgag gttgaccacc
     3781 gggatcacga acggacccag cgccgccggg gtgaggtcga cgaggcgctt gccgtggggc
     3841 gccagcgcct ccgcgttggc ctcgtggccc ttcgccgagg tcgcgtcgag gacgatctcg
     3901 atcgcctcga actccggcat ctcgatcagc ccggcgaccc cgtcggaggt cgtcgcgacc
     3961 cccatccggc cggcccgggc gagcccgtcg gagtcggggt cgatcccgac catcgcggcc
     4021 acctcgagcg cctcggagcg gcgcaccttg aacatcaggt cggtgccgat gttgcccgag
     4081 ccgatgatcg cgactttggt cgctgtcatt tcatcctcct tggtcgaatc tggcggtgac
     4141 cgtcccgagg gacgagatcg aagcggtcag ctcgtcgccg gggaagacca cggcgagcgg
     4201 gccgagggcg ccggaaagaa ccacgtcgcc ggcgcccagc ggggagccgt agcgggcggc
     4261 cgcctcggcc agccaggcga gcgcgttgag cgggtcgccg aggcaggcgg cgcccgtgcc
     4321 ggaagagatc tcctcgcccg cgcgggtcag cgacatcgtc accgcgggcg ggtcgaagtc
     4381 ggcgagcgcg acgcgctcgg cgccgagcac gaaggcgccg ctggaggcgt tgtcggcgac
     4441 cgtgtcggtg atcttgatgt cccagtcgcg cacgcggctg gcgacgacct cgatcgcggc
     4501 gacggcgtag tcgaccgcgg cgcgcacctc ctcgggatcg gcgacgtcgg cgatcggcgc
     4561 ggcgagcacg aaggcgatct cgacctcggc ccgcggctgc agcagcgagg agtaggggat
     4621 cgtctcgccg tcggcgtagc tcatctcgtc gagcaggaag ccgaagtcgg gctggtcgac
     4681 gccgacctgt tcctggaccg cttttgaggt cagcccgatc ttgcggccga cgaccttggc
     4741 accaccggcg aggcgggcgg cgatcacctg ttgctggacc cggtaggcga ggtcgatgtc
     4801 ggtggcgccg atcagctcgc ggaccggctc cgccggcgtg ccgctctcct gggcacccag
     4861 cagccgctgt gcggcttcct cgattgaccg ttgcatgctc tccatcagtc cgacccagcc
     4921 gatgcgtgcg tgtaaataac gcacagcaac atccgctaag tacacaacct accagacgcc
     4981 gagcagcgtc tgtcaagccc cggttaccgg gccggaccga cggcggaggg ggagtgcgct
     5041 cagccgagga tgccggcgag ctcgcgcgcc gcctcgacca ccgtcgggcg cgcctcttcg
     5101 agcacgtcgg gacggtggga gacgaggttg atcgcggtcg ccggcgaggg cggcgtgcgt
     5161 ttgaccggca ccgcgaggcc gtaggcgttg ggctcgacct cgccgtgggt gaggacccag
     5221 ccctgcttgc gcgcttcggc gacgacctcg cgctcctccg gctcgggcgg ggcgctggcc
     5281 agcagcgcca gcccggcggc gccgcggtcg atcgggtagc ggctgccctc gcggaaggag
     5341 aggtggtagg agaggccggc ggggacgatc accgcgatcg ccacctgctc ctcgccctcg
     5401 gcgaccagca gcgaggcggt cgcctgggtg ccgtcggcga ggcgtcgcaa gaccggcatg
     5461 ctgacttcgc gcaggttctg gtcgaaggag gcgccgagcg cggcgaggcc ggcgccggga
     5521 cggaagcggc cttcgtggcg ggcgatcagg tggaactgct cgagcgtcgc cagcagccgg
     5581 gcggcgacgg tgcggtggat ctccagctgc tcggcgacct cctggatcgt caatccgccg
     5641 ggcgaatcgc agatcaggtg cagggcggtg aggccccggg cgagcgtctg tgaaccggct
     5701 ggggttcgtc ctttgtccct gctcggcacg gggccaatat agccacacgc cggggcgaaa
     5761 cgcttgacaa ggtcccggac ggagtaaata ctgcctctgc agatatcgca ctccaatgtg
     5821 ctatagatgc acaccggaaa gggtctgtgg aggatttcga gagcatctgg cgtgacttgc
     5881 aagccgtgcc gtttgagcag ggctacctcg acgttggcgg ctgccgtacg cggtatctgc
     5941 acgccggcgc ggaggaccag ccgaccctgg ttttgctgca cggatcggcc ggacacgccg
     6001 aggcctacgt gcgcaacctc gacgcccacg ccgcccactt ctccacctgg gcgatcgaca
     6061 tggtcggcca cggcttcacc gacaagcccg ggcatccgct cgaggtcgag cactatgtag
     6121 agcacctgct cgccttcctc gacgagatcg gggtcgagcg cgcccacctc tccggcgagt
     6181 cgctcggcgg ctgggtagtc agccgggcgg cgatcgacca tcccgagcgc gtcgaccgcc
     6241 tcgtgctcaa caccgccggc ggctcgcagg ccgatcccga ggtgatggcg cggatccgca
     6301 cgctctcgat ggcggcggcg gaaaaccccg actgggagac cgtcggcgcc cgcatccgct
     6361 ggctgatggc cgacaaggag cgcaactacg acgacgtgat cgccagccgc caggcggtct
     6421 accggcagcc cggcttcgtc ggggcgatgg acgacatcct cgccctccag gaccccgaga
     6481 tccgcgcccg caacatcgtc tcgcccgagg actacgggcg gatcgcggcg ccgaccctgg
     6541 tgctctggac cagcgacgac ccgaccgccg acgtctccga gggccgccgg atcgccacga
     6601 tgatccccgg cgccgagttc gtcgtgatgg agggctgtgg gcactggccg cagtacgagg
     6661 acccggcgga gttcaaccgc attcacctcg acttcctgcg gggctcggca tgaggcccga
     6721 cgtggtcatc gtcggtgccg gcccggtcgg cctcaccctc gccaacttcc tcggcatgca
     6781 cggggtgcgc acggtcgtcg tcgagcagcg cgaggagctg atcgactacc cgcgcggggt
     6841 cgggatcgac gacgagagct tccgcgcctt ccaggcgatc gggctgatcg agccgatcct
     6901 gccgcacacg gtgcccaacc aggtgatgcg cttcgtcaac ggcaagggcc gcgtcctggc
     6961 cgagatcgcg cccagcgagc agccgttcgg ctggccgcgc cgcaacggct tcatccagcc
     7021 actggtcgac gagcagctgc tggccggcct cgaccgcttc gagtgcgtcg aggtgctctg
     7081 ggagcggacg ctggagtcgc tggcgacgac ggcggacggc gccgcggtca cggtcagcgg
     7141 ccccgacggc ccggagacga tccgggcccg ctacgtggtc ggctgcgacg gcggccgcag
     7201 caccacccgg cggctgaccg agacctcgtt cgagggcacg acctcggcca cccgctgggt
     7261 cgtgatcgac ctccgcaacg acccgctcgg cagccccaac gtgaccgtcg gcgccgaccc
     7321 ggcgcgcccc ttcgtctcgg tctcgctcgc ccacggcgtg cgccgcttcg agttcatggt
     7381 ccacgacgac gagtccgacg agcaggtcga ggaccccgag ttcctcgccg agctgctgcg
     7441 cccgcacgtc ccctatccct cgcagatcga cgtgatccgc aagcgcgtct acacccacca
     7501 ctcgcggatc gccggcagct tccgcgccgg ccccgtcttc atcgccggcg acgccgctca
     7561 cctgatgccg gtctggcagg gccagggcta caacagcggg atccgcgacg ccgtcaacct
     7621 cggctggaag ctcgccctgg tgatccgcgg cgctgccggc gacgccctgc tcgacagcta
     7681 cgaccccgag cgccgcgacc acgcgcgggc gatgatcgac ctctcgacca cggtcggcaa
     7741 atgggtctcg ccgaccagca cccgggtggc ggcgctgcgc gacgtggtcc tgcgcgtgat
     7801 ctcggcgatt cccagcttca agcgctacct ggtcgagatg cggttcaagc cgatgccgcg
     7861 ctacgcccgc ggtgcgatca ccgagctgca cgccgccgac gacggcgtcg tcgggcgcca
     7921 gttcatccag ccgcgggtcg acacccgcga gcgccgcggg gtgcagctcg acgaggtcac
     7981 cggcccctgg ttcagcgtgc tctgctggaa caacgacccg cgcgcggtgc tcggcgagga
     8041 ggccgagcgc tggctggcgc tcggcgcccg cctgatcgag ctgcgcccgc agtcgcagct
     8101 gcactgggac ggcagcggcc gcgagggggt agaggtcgtc ggcgacgcga gcggggcgct
     8161 gaagaagtgg ttcgacggcc agcgctcctc cgtcctcttc ctgcgcccgg accgcttcgt
     8221 cgccgccgcc tgcgtggccc agcacgctcc cgaggtctcg cgcgcgctcg cctcggcgct
     8281 ctccctgacg gaaggcagcg caaagcttga accggccgcc ccggcggcgc gaggataggc
     8341 ggcatggagc ccggcttcga ccagagcgtt gacctcctgg tcgtcggctc cggcggcggc
     8401 gggctgacca cggctatggc ggcgaaggcc gccggcctgg agacgctgct ggtcgagaag
     8461 tccgagcgct tcggcggctc gaccgcgctc tcgggcggcg gcatctggat ccccggcgcg
     8521 agcgcccagg tgcgcgccgg ctaccggccc gatccggagg agacgctcga gtatctgcgc
     8581 acgatcacca aaggcgtcgt ctccgaggag cgcctgcgcg cctacgtcga gaccggcccg
     8641 gagatgctcg acttcatcga acgccacacc gacgccgagc tggtctggaa gcccggctac
     8701 ccggactact tcccggagga gccgggcggc tcggccgagg gcagcgtgat caacctcgag
     8761 ccgatcgacc tgcgcaagct cggccccgac gaggagaagc tcttcggccc ggcggcggcc
     8821 ggcccgaagg ggatgtggct gcggccgctg gagctgaagg agttcttcac gatccgccag
     8881 tcctggcgcg gcaagctggt cctgctgcgc ttcctctggc gctcgctgcg cgcccgcgtc
     8941 accggcgagc gcgtggtcgc gctcgggcag gcgctggtcg cgcagctctg gctcgggctg
     9001 cgcaggctcg gcgttccggt ctggctcggc tcgccgctgg tctcgctgat caccggcgag
     9061 gacggcgcgg tgaggggcgc ggtgatccgc cacgagggcc gcgaggtccg ggtcgaggcc
     9121 cgcggcggcg tcgtgctcgc gaccggcggc ttcgagcaca acgaggagat gcggcgccag
     9181 taccagccgt tcgtccccga ggacttcagc ctcggcaacc cggccagcac cggcgacggg
     9241 atccgggccg ggcaggaggc cggtgcggcg ctcgacctga tggacgaggc ctggtggttc
     9301 ccgaccctgg cctggcccag cgggcggctg cacatgatgc tgaacgagcg gatgatgccg
     9361 gcccagttca tcgtcaacgg tgccggcgag cgcttcatca acgaggcgac cccctactcg
     9421 gagttcggcc acgcgatgat cgagggacag gcgaccggca ccacctacat gc
//
LOCUS       DASRIG010000437         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3871050, whole genome shotgun sequence.
ACCESSION   DASRIG010000437 DASRIG010000000
VERSION     DASRIG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3871050"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFB52_11690"
     CDS             <1..474
                     /locus_tag="VFB52_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZO07039.1"
                     /translation="LLPGQLEDRGALKRLNKKTKEEGGTPAKGEEVILGITKASLATE
                     SFLSAASFQETTKVLTDAALEGKRDRLVGLKENVIIGKLIPAATGLKHYRTVAIEPAV
                     AVEPPSDEDLLEEDELAAELGVDGVETPVEGFGLSFAEELEELAQEIESTSEESK"
     gene            584..1354
                     /locus_tag="VFB52_11695"
     CDS             584..1354
                     /locus_tag="VFB52_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07040.1"
                     /translation="MLCERRDQVVAGVLALCAVIALILAATAYGAGEYEPNDSRETAA
                     GPLAGGTDYTATFETDNDVDWYVFYIKVYSQMDFSATTVGGCGSGYIYILDKDGKSVD
                     SLRIGSLNQVNHRLLTLPAGRYYLDVTNEYGCTSDRYKLRIDPAAAVTPSKVCGEAIV
                     AREAVGPELAKLNEKLGYVNNVVVNRTAKVQATKKRLKRLKKNRASRYAKADARRDLA
                     AAKTSLNKALEKKLALETLGGQYAASLNTANAQIATSC"
     gene            complement(1419..2096)
                     /locus_tag="VFB52_11700"
     CDS             complement(1419..2096)
                     /locus_tag="VFB52_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07041.1"
                     /translation="MTSMTRKFRISPAMILSIVALFVALGGSAYAVGIGKNTVRSPQI
                     VDGTVRTVDVRDNAVNAAKIAPDAVGNDELAENSVTSQQVLDETLTNQDLGAASVTSS
                     EIADGSVTTTDVADQGLTATDLGANSVGSSELQSSSVRAAELGTIIQVSQTEAIKANT
                     NKTAAILCPEGTTVISGGGFGTLYSIHMSGSFRNGNGWRVDAKNNSGTDGGITAYAYC
                     LSGGSSN"
     gene            2311..>3608
                     /locus_tag="VFB52_11705"
     CDS             2311..>3608
                     /locus_tag="VFB52_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZO07042.1"
                     /translation="MERSDRVCVIGAGSSGISSCQVLNARGIPFDCFEKGSEVGGNWR
                     YENDNGMSSAYRSLHINTSRDLMAFRTYPMPDSYPDYPNHFQIAAYFDSYVDRFGFRD
                     KIAFRTEVVAVEPVDGEWEVTVEDAGGNRETRRYRAVLVANGHHWNPRWPEPAFPGSE
                     EFEGEQMHVHHYREPEVLRGKRVLVLGIGNSATDIAVESSRIGERTFLAMRRGAYVLP
                     KYMNGKPTDESASAWLTRMPLPVQRFFLTRMVGLTAGDMTAYGLPKPDHKLLEAHPTI
                     SAELLSRLGHGDIEVKPNIERYAGGRSVRFVDGSEEEIDLVIYCTGYKITFPFLDAKL
                     LAARDNNLPLYRRVVSIENPGLYFIGLIQPLGAIMPIAEAQSEWVADLLEGRGSLPGA
                     GEMREAVTETEQRMRKRYVASKRHTIQVDFHPYLREIRRER"
BASE COUNT          623 a         1201 c         1213 g          571 t
ORIGIN      
        1 ctgctgccgg gccagctcga agatcgcggt gcgctcaaac ggctcaacaa gaagaccaag
       61 gaagaaggcg gcaccccggc caaaggcgag gaggtcatcc tcgggatcac caaggcctcg
      121 ctggcgaccg agtcgttcct ctccgcggct tccttccagg agacgaccaa ggtgctcacc
      181 gacgctgccc tcgagggcaa acgggacagg cttgtgggcc tgaaggagaa cgtgatcatc
      241 ggcaagctga tcccggcagc caccgggctc aagcactacc gcacggtcgc gatcgagccc
      301 gcggtggcgg tcgagccgcc cagcgacgag gacctgctcg aagaagacga gctggccgcc
      361 gagctcggcg tcgatggcgt cgagacgccg gtcgagggct tcggcctctc ctttgcggag
      421 gagctcgagg agctcgcgca ggagatcgag tcgacctcgg aagagtcgaa ataggcgctc
      481 gcaaattccg cagtgaaaag gggccccgcg gggccccttt tctgtggctt gaggaaccgc
      541 agcgttagtc ccggttattc gagcgttcaa taggtttccc gccatgctgt gcgagcggcg
      601 ggaccaggtc gtggcgggag tgctggcgct gtgcgcggtg atcgcgctga tcctggcggc
      661 gaccgcctac ggtgccggag agtacgagcc caatgattcg cgggaaaccg ccgcgggccc
      721 gctcgcaggg ggaaccgact acacggccac gttcgagacc gacaacgacg tcgactggta
      781 cgtcttctac atcaaggtct acagccagat ggacttctcc gcgaccaccg tcgggggttg
      841 cggatcgggc tacatctaca tcctcgacaa ggacggcaag tcggtcgaca gtctcaggat
      901 cggctcgctg aaccaggtca accaccgtct cctgaccctg cccgccggcc gctactacct
      961 ggacgtcacc aatgaatacg gctgcacgag cgaccgctac aagctccgca tcgaccccgc
     1021 ggccgccgtg accccgagca aggtctgcgg cgaggcgatc gtcgcgcgcg aagcggtcgg
     1081 gcccgaactg gcgaaactga acgaaaaact cggctacgtg aacaacgtcg tcgtcaaccg
     1141 gaccgcgaag gtgcaggcga ccaagaagcg cctgaagcgc ctcaagaaga accgcgcttc
     1201 tcgctacgcg aaggccgacg cgcgccgcga cctcgccgcc gccaagactt ccttgaacaa
     1261 ggcgctggaa aagaaactgg ccctggaaac cctcgggggc cagtacgcgg ccagcctgaa
     1321 caccgccaac gctcagatcg ccacctcctg ctgaacgcga aaggccgagg accctgaggt
     1381 ccccggcctt tcccggtggc gcgagtgagg cttcgcgtct agttgctgct gccgccgctg
     1441 aggcagtagg cgtaggcggt gatgccgccg tcggtgccgc tgttgttctt cgcgtcgacc
     1501 cgccagccgt tgccgttgcg gaacgagccg gacatgtgga tcgagtagag cgtgccgaag
     1561 ccgcctccgc tgatcacggt cgtgccttcg gggcagagga tcgcggcggt tttgttcgtg
     1621 ttcgctttga tcgcctcggt ctgggagacc tggatgatcg tccccagctc ggcggcgcgg
     1681 acagagctgg actgcagctc cgacgagccg accgagttgg cgccgaggtc ggtggcggtc
     1741 agcccctggt cggcgacgtc ggtggtggtg acgctgccgt cggcgatctc cgaggaggtc
     1801 accgaggcgg cgccgagatc ctggttggtc agggtctcgt cgagcacctg ctgagaggtg
     1861 acgctatttt cggcgagctc gtcgttgccg accgcgtcgg gggcgatttt cgcggcgttg
     1921 accgcgttgt cgcggacgtc cacggtgcgg acggtcccgt cgacgatctg cggcgagcgc
     1981 acggtgtttt tgccgatccc gaccgcgtag gcgctgccgc cgagggcgac gaacagagcg
     2041 acgatcgaga ggatcatggc gggcgagata cggaacttgc gggtcatgct ggtcatcgga
     2101 gtggtccttt cggttggttg gttgatccga tgggtccagc ttcggcccga gcgcccgccg
     2161 caactacgga ccccagggtg gaaccgcgcc cccgccgggg tggcctcact cacccctggg
     2221 gctggcttcc gccggctccc cgctggcccc tgggcccgcg ggactggcca gccagccagt
     2281 cgacgaccgg tttcagatag attcagcgcg atggagaggt cggatcgcgt ctgcgtcatc
     2341 ggagcagggt cctcggggat ctcctcctgc caggtgctga acgcacgcgg gatccccttc
     2401 gactgcttcg agaagggctc ggaggtgggg ggcaactggc gctacgaaaa cgacaacggg
     2461 atgtcgtctg cctaccgctc gctgcacatc aacacctcgc gcgacctgat ggccttccgg
     2521 acctatccga tgccggacag ctacccggac tatcccaacc acttccagat cgccgcctac
     2581 ttcgactcct acgtcgaccg cttcggcttc cgcgacaaga tcgccttccg caccgaggtg
     2641 gtcgcggtcg agccggtcga cggcgagtgg gaggtcaccg tcgaggacgc cggcgggaat
     2701 cgcgagacgc gccgctaccg cgccgtgctc gtcgccaacg ggcaccactg gaacccgcgt
     2761 tggcccgaac ccgccttccc cggatccgag gagttcgagg gcgagcagat gcacgtccac
     2821 cactaccgcg agccggaggt gctgcgcggc aagcgggtgc tggtgctcgg gatcggcaac
     2881 tcggccaccg acatcgcggt cgagtcatcg cggatcggcg agcggacctt cctggcgatg
     2941 cgccgcggcg cctacgtgct gcccaagtac atgaacggca agccgaccga cgagtcggcc
     3001 tcggcctggc tgacgcggat gccgctgccg gtccagcgct tcttcctcac ccgcatggtc
     3061 ggcctcaccg ccggcgacat gaccgcctac gggctcccca agcccgacca caagctgctc
     3121 gaggcccacc cgacgatctc cgccgagctg ctctcgcggc tcggccacgg cgacatcgag
     3181 gtgaagccga acatcgagcg ctacgccggc ggtcgcagcg tccgcttcgt cgacggcagc
     3241 gaggaggaga tcgacctcgt catctactgc accggctaca agatcacctt ccccttcctc
     3301 gacgcgaagc tgctggcggc gcgcgacaac aacctgccgc tctaccggcg ggtggtgtcg
     3361 atcgagaacc cggggctcta cttcatcggc ctgatccagc cgctcggggc gatcatgccg
     3421 atcgccgagg cgcagtcgga gtgggtcgcc gacctgctcg aagggcgcgg ctcgctaccg
     3481 ggggcggggg agatgcggga ggcggtgacg gagaccgaac agcggatgcg aaagcgctac
     3541 gtcgcctcta aacgtcacac gatccaggtc gactttcatc cttacctgcg cgagatccgg
     3601 cgcgagcg
//
LOCUS       DASRIG010000438         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1254533, whole genome shotgun sequence.
ACCESSION   DASRIG010000438 DASRIG010000000
VERSION     DASRIG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254533"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFB52_11710"
     CDS             complement(<1..653)
                     /locus_tag="VFB52_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07043.1"
                     /translation="MTTVGVGVGGCSGDTEHQLESVASAAEVRWEDDPGDPLEVGPLD
                     GQNGWIRAPGFGSADVVTWAADNRVIKLASDVDGQPRVVMGKEVLRQTAGHHVLELDV
                     MVEHPNLANLTTLDLNTDPRTVEECEWATKKLRFYFGSAIRIETGPGPDPVSDTLVPQ
                     VVAGQWYHLRADIDLNNEVVSVWLGADLTAVNNRPLGPGPIVGLSLAGWDGVGQANA"
     gene            complement(796..1122)
                     /locus_tag="VFB52_11715"
     CDS             complement(796..1122)
                     /locus_tag="VFB52_11715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZO07044.1"
                     /translation="MIESRRTFRRLVDITATIAIGDGAPEPCHIENLSLTGAFVSGRR
                     MTSGDRIRLSFKLPQLEEAIESVATVRWSNGSGIGVQFDGLRARQVWALGKFFEQETR
                     EQQARP"
     gene            1498..>2437
                     /locus_tag="VFB52_11720"
     CDS             1498..>2437
                     /locus_tag="VFB52_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HZO07045.1"
                     /translation="MALPDHSLSRRRGVDLLLIAAAVAALAACTTDVSSQEGSTEPNP
                     NERSASPAADYSILRPGNRYAADVGDRYLDEIQPTLASRCAVCHSCTNGPCQLNMTSF
                     AALQRGIHSKNPYKWGLTDNLQTRVSDNRPLSEWRAKGFKSVLPDGGDPESSIFWKAL
                     ELGDQNTSSPDPNQGPLATSQVRRLAQDHDAGNYSCPSNSFEWWLFTWKYPQGGMPWG
                     LPTDADAHATLEEWTLDGAPGPSAAAQRTVSSPQRTVMTQMDPDALVATWDAFLDGSD
                     LRSKLVARYIFEHAYSANIHFDENPGEFYRIVRSRTA"
BASE COUNT          394 a          862 c          796 g          385 t
ORIGIN      
        1 aaggcgttgg cctggccgac gccgtcccag cccgcgaggc tgagaccgac gatcgggccg
       61 gggccgaggg ggcggttgtt caccgcggtg aggtcggcac ccagccagac gctcaccacc
      121 tcattattga ggtcgatgtc ggcgcgcagg tggtaccact gtccggcgac cacctggggg
      181 accagcgtat cgctgaccgg gtcggggccg ggcccggtct cgatgcggat cgcgctgccg
      241 aaataaaagc gcaacttttt ggtcgcccac tcgcactcct cgaccgtgcg cggatcggtg
      301 ttgagatcga gggtggtcag gttggcgaga tttggatgct cgaccatcac gtcgagctcg
      361 agcacgtggt gcccggcggt ctgccgcagc acttccttgc ccatcacgac gcggggctgg
      421 ccgtcgacgt cgctggcgag cttgatgacg cggttgtcgg cggcccaggt gaccacgtcc
      481 gcgctgccga agcccggcgc gcgaatccag ccgttctgac cgtccagtgg cccgacctcg
      541 agtgggtcac cgggatcgtc ttcccagcgg acctcggccg cggaggcgac cgactcgagc
      601 tggtgttcgg tgtcgccgga gcacccacct acccctactc cgaccgtagt caatgtaaga
      661 gcagaaagaa tcgtagcgaa caaaaaacgt tcgttacgaa cacaattaga atgctcccgt
      721 atcatctcgc ccccgagaga agtcttcggt tccttctcac ggagacggcg ttcaggtcaa
      781 cgttcgtttc ctaagctacg gccgggcctg ctgctcccgc gtctcctgct cgaagaattt
      841 ccccagcgcc cacacctggc gcgcgcggag gccatcgaac tgcacgccga tgccgctgcc
      901 gttgctccag cggacggtgg cgacgctctc gatcgcctcc tccagctgcg gcagcttgaa
      961 cgagaggcga atgcgatcgc cggaggtcat ccggcgcccg gacacgaagg cgccggtcag
     1021 cgagaggttc tcgatgtggc acggctcggg cgcaccgtcg ccgatggcga tggtggcggt
     1081 gatgtcgacg aggcggcgga aggtgcgacg agactcgatc atgaaggcgc gcccattcta
     1141 gcgatgaggt tgacggcgga ggatgcactc tccggtgcga agcggcaaac gttgctgctc
     1201 acccagcgac ggcgatcgat cgcgacgagc gaaccgcggt cgttgatctc atcgggggag
     1261 acgcgccgcg gtcgattgcg gttgcagcga tcgcgacgtg cggcgcgcgc cgcgcgaccg
     1321 gggcagcgct gcccgccgcg cgcgcctcga taggtcgatc tgattccgct tcggcgcggc
     1381 cgtctccgtc gaggacccac accgcttgtg ggccggctca ctggggattc agcaccgcga
     1441 aatgtgtccg aatttcggcc ttgagctggg tctcgctcat ccctaaggtg ccgcgccgtg
     1501 gcactacctg atcattcgct ttcacgccgg cgcggcgtcg atctcctgct gatcgccgcc
     1561 gccgtcgccg ccctcgcggc gtgcaccacc gacgtctcgt cgcaggaggg ctcgaccgag
     1621 ccgaacccca acgagcgcag cgcgtcgccc gccgccgact actcgatcct ccggcccggc
     1681 aaccgctacg ccgccgacgt cggcgatcgc tacctcgacg agatccagcc gacgctcgcc
     1741 agccgctgcg cggtctgcca ctcgtgcacc aacggcccgt gccagctgaa catgacgtcg
     1801 ttcgcggcgc tgcagcgcgg catccactcc aagaacccgt acaagtgggg cctgaccgac
     1861 aacctgcaga cccgggtcag cgacaaccgg ccgctgtccg agtggcgggc gaagggcttc
     1921 aagtcggtcc tgcccgacgg cggtgatccc gagagctcca tcttctggaa ggcgctcgag
     1981 ctcggcgacc agaacacgtc gtcgcccgac ccgaaccagg gcccgctggc gaccagccag
     2041 gtccgccggc tcgcccagga ccacgacgcc ggcaactact cgtgcccgtc gaacagcttc
     2101 gagtggtggc tcttcacctg gaagtatccg cagggcggca tgccgtgggg cctgccgacc
     2161 gacgccgacg cccacgccac cctcgaggag tggaccctcg acggcgcgcc cggcccgtcg
     2221 gccgcggccc agcgcaccgt gtcgagcccg cagcgcacgg tcatgaccca gatggatccc
     2281 gacgcgctgg tcgcgacctg ggacgccttc ctcgacggca gcgacctccg ctccaagctg
     2341 gtcgcccgct acatcttcga gcacgcctac tcggccaaca tccacttcga cgagaacccg
     2401 ggcgagttct accggatcgt ccggtcgcgg accgcgg
//
LOCUS       DASRIG010000439         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2021942, whole genome shotgun sequence.
ACCESSION   DASRIG010000439 DASRIG010000000
VERSION     DASRIG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021942"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFB52_11725"
     CDS             complement(<1..420)
                     /locus_tag="VFB52_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO07046.1"
                     /translation="MSAESKVKALMERELATFEAAHPRSRQIHEEAGEALLSGVPMNW
                     MTRWPGSHPVFFAEAEGNRLRDVDGNELVDFCLGDTGAMTGHSPPPTVAAAQARLERG
                     ITTMLPSEDALPVGEEMSRRFGLRHWQFSLSATDANRF"
     gene            519..1811
                     /gene="eno"
                     /locus_tag="VFB52_11730"
     CDS             519..1811
                     /gene="eno"
                     /locus_tag="VFB52_11730"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZO07047.1"
                     /translation="MTLATPVITGLAAREVLDCRGLPTLQVDVELDGAITGTADVPSG
                     RSTGSNEAFELRDGGERFGGFGMLQAAGNVTGEIADALLGTELRSQRQLDRDLVALDG
                     TENKSRLGANAVLGVSLAASRALADFNGLPLYRSVNSNGHILPVPLVNLINGGKHASN
                     DLDFQEFIVIPVGADSILEALQISTETNLALAEILLERYGKVALNTGDEGGFAPPISD
                     PNEALSLLHEAVAAAGHEGRFRYGLDCAATHYYDADSGTYEIDGVSRDRDAMIEMYQE
                     LIAKYDIVTIEDPLDEEDFEGFAALTEAAGIQIVGDDLFTTNPKRLAQGLEVGAANSL
                     LWKFNQIGTLSEALDAAEMAHKAGYSVVVSERSGETEDAIIADLSVALGAGQIKTGAP
                     VRGERTAKYNRLIKIAEELGETAVYPGDTYGARKSAVA"
     gene            1857..>2062
                     /locus_tag="VFB52_11735"
     CDS             1857..>2062
                     /locus_tag="VFB52_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO07048.1"
                     /translation="MTDDKNPIAPITPVSTVDAAANALRELILDGKLEPGTRLREHEF
                     AERLGIARHSFRAATQILIGEGLLR"
BASE COUNT          356 a          738 c          675 g          293 t
ORIGIN      
        1 gaaccggttg gcgtcggtcg ccgagagcga gaactgccag tggcgcaggc cgaagcggcg
       61 gctcatctcc tcgccgaccg gaagcgcgtc ctcgctcggc agcatcgtcg tgatcccgcg
      121 ctcgagccgc gcttgcgcgg cggcgacggt cggcggcggc gagtggccgg tcatcgcgcc
      181 ggtgtcgccg aggcagaagt cgaccagctc gttgccgtcg acgtcgcgca gccggttgcc
      241 ctcggcctcg gcgaagaaga cggggtggga gcccggccag cgggtcatcc agttcatcgg
      301 caccccggag agcagcgcct cgccggcttc ttcgtggatc tgccgggagc gggggtgggc
      361 cgcctcgaag gtcgccagct cgcgctccat cagggctttg accttgctct cagcgctcat
      421 ccgcactcct ctgttagatt gtggaacgat tcgacaaaca agatacaagc agagatcaag
      481 caagtgacga tccaccggac gatcaaggag aaccaagaat gacgctggcc acccccgtaa
      541 tcaccggcct ggccgcccgc gaggtgctcg actgccgagg ccttcctacc ctgcaggtcg
      601 acgtcgaact cgacggcgcc atcaccggca ccgccgacgt tcccagcggc cgctcgacgg
      661 ggagcaacga ggcgttcgag ctgcgcgacg gcggcgagcg cttcggcggc ttcgggatgc
      721 tgcaggcggc cggcaacgtg accggcgaga tcgccgacgc gctgctcggg accgagctgc
      781 gctcccagcg ccagctcgac cgcgacctgg tcgccctcga cggcaccgag aacaagagcc
      841 ggctcggcgc caacgcggtg ctcggcgtct cgctcgccgc ttcccgcgcg cttgccgact
      901 tcaacgggct gccgctctac cgctcggtca actccaacgg ccacatcctg ccggtgccgc
      961 tggtcaacct gatcaacggc ggcaaacacg cctccaacga cctcgacttc caggagttca
     1021 tcgtgatccc ggtcggcgcc gactcgatcc tcgaggcgct gcagatctcg accgagacca
     1081 acctcgcgct ggccgagatc ctgctcgagc gctacggcaa ggtcgcgctc aataccggcg
     1141 acgagggcgg cttcgcgccg ccgatctccg accccaacga ggcgctctcg ctgctccacg
     1201 aggcggtcgc cgcggccggc cacgagggcc gcttccgcta cgggctcgac tgcgccgcga
     1261 cccactacta cgacgccgac tcgggcacct acgagatcga cggggtcagc cgcgatcgcg
     1321 acgcgatgat cgagatgtac caggagctga tcgccaagta cgacatcgtc acgatcgagg
     1381 atccgctcga cgaggaagac ttcgagggct tcgcggcgct gaccgaggcc gcggggatcc
     1441 agatcgtcgg cgacgacctc ttcaccacca acccgaagcg cctcgcccag ggcctcgagg
     1501 tcggcgcggc caactcgctg ctctggaagt tcaaccagat cggcacgctc tcggaggcgc
     1561 tcgacgccgc cgagatggcg cacaaggccg gctactcggt ggtcgtctcc gagcgctcgg
     1621 gggagaccga ggacgcgatc atcgccgacc tctccgtcgc cctcggcgcc ggccagatca
     1681 agaccggcgc gccggtccgc ggcgagcgca ccgccaagta caaccgcctg atcaagatcg
     1741 ccgaggagct gggggagacc gcggtctatc ccggcgacac ctacggcgcg aggaagtcgg
     1801 cggttgcctg aggaaggccc gctaaagctc tgtctaattg tggaagaatt ggacacatga
     1861 ctgacgacaa aaaccccatc gctccgatca cgcccgtctc caccgtcgac gccgcggcaa
     1921 acgccctgcg tgagctgatc ctcgacggca aactcgagcc cggcacccgg ttgcgcgagc
     1981 acgagttcgc cgagcggctg gggatcgccc gtcactcgtt ccgggcggca acccagatcc
     2041 tgatcggcga ggggctgctg cg
//
LOCUS       DASRIG010000440         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_444657, whole genome shotgun sequence.
ACCESSION   DASRIG010000440 DASRIG010000000
VERSION     DASRIG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444657"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFB52_11740"
     CDS             <1..259
                     /locus_tag="VFB52_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07049.1"
                     /translation="SGRRGEALRIINELEGQAARRQVSPVYIAKIYAGLGDKDRAFEW
                     LRRGYAERSDHLVSLRVDPAYDSLRSDPRFVDLLRSVGLAP"
     gene            614..>2550
                     /locus_tag="VFB52_11745"
     CDS             614..>2550
                     /locus_tag="VFB52_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZO07050.1"
                     /translation="MPLLTLLVLSASLAAKAPAQSIGRVAGTVTDNTGAAVVDAAVEV
                     VNLETGEVRRVTTNASGTYAVSPLPVGAYRVQIRKAGFKAATRNDVRVDVNSAATVDV
                     QLEVGSMTESVTVEAQSAAIETETAAVGNSRYETQLKQLPVIVREVQTLVGQTAGVPY
                     GTTDTVGGSFNQGGRSAMQIVADGAQLNPLQTTGWPAIDGIGRRADLSMPSLDALQEV
                     RWATNGGNAEYAQPTQVIAASKSGTNALRGSLFEFYRSGGMGARRWEAASRESFVRHQ
                     FGGTVGGPIRRDKMFFFGSADVFRHTSNLVLNARYPTAAERAGDLSSLLARTDAAGGP
                     APIRVIDPLTGQPFPGNVIPASRIDPVAAELLRLIPDAPRPGRLTDFNAVYTKPQFDN
                     SEKYDARFDYNVTQQHRVFARATVARLDQASRYSGDVPGPHGSSTKKQWNYTVAANWT
                     SVLSPSTIAAFQFSYRNLPFRNIPSVGDELFPVPINDVRPAPPYAGPPAIAIGSNGLG
                     ISTLFDRLLFNVSEDYGYTLDPNLTKVIGNHTLKAGFTYLRGHKTTEIASPPYGRFTT
                     VSDFNNARSTTSATGDAFADFLLGYPSSTDVTIGEVGGFHSKTNYHAYLQDDWKVTPR
                     LTLNLGLRYDNFGFFEEEFGRA"
BASE COUNT          431 a          890 c          855 g          374 t
ORIGIN      
        1 gtccggccgg aggggggagg cgctgagaat catcaacgag ctggaagggc aagccgcgcg
       61 gcggcaggtc tcccccgtct acatcgccaa aatctacgct gggctcgggg ataaggatcg
      121 ggcgttcgag tggctgcgcc ggggctacgc agagcgctcc gaccacctgg tctcgctgcg
      181 cgtcgatccg gcctacgaca gcttgcgctc ggacccgcga tttgtcgatc tcttgcggag
      241 cgtcggcctc gcgccgtaag ccgcaaggcg cccccgaaga atcagcccct gccgaccctt
      301 cgcgccaccc agccgcggcc tcgcctcact cgccgctaaa cctaacggcc acaataacta
      361 cccaggagga cgccttccat tgacattcct tttgacatat cagaagatgc gatcctcgaa
      421 gaccagccgc ggggacgctg tggccgagtg cccggggccg gcgtccggag acactgtgtt
      481 agacgtatcg accgcagaag gtcaacgtat tcacgggtca tcgtcccgat ttaggtttcg
      541 cagcactgac atatcaacac ctgtggtaga tcatggccgg ccgcgcgccg ggcccgaagg
      601 agtcgccatg cgagtgcctc tactcaccct gctcgtcctg tccgcgagcc tggcggcgaa
      661 ggcgccggcg cagagtatcg ggcgcgtcgc ggggaccgtg actgacaaca ccggcgccgc
      721 tgttgtcgat gccgcggtcg aggtggtgaa tctggagacc ggcgaggtgc ggcgcgtgac
      781 gaccaacgcg tccggcacct acgccgtgtc gccgctcccg gtcggcgcct accgcgtgca
      841 gattcgcaaa gcgggcttca aggccgcgac gcgcaacgac gttcgcgtgg acgtcaactc
      901 ggcggcgacc gtcgacgtgc agctcgaggt cgggagcatg acggagagcg tcaccgtgga
      961 ggcgcagtcg gcggcgatcg agacggagac ggccgcggtc ggcaactcgc gctacgaaac
     1021 gcagctcaag cagctgccgg tgatcgtgcg agaggtccag acgctggtcg ggcagacggc
     1081 gggcgtgccg tacgggacaa cggacacggt cggcgggtcc ttcaaccagg ggggccggtc
     1141 ggccatgcag atcgtcgccg acggcgcgca gctcaacccg ctccagacta cggggtggcc
     1201 ggcgatcgac ggcatcgggc gccgggcgga cctctcgatg ccgagcctgg acgccttgca
     1261 ggaggtgcgg tgggcgacca acggcggcaa tgcggagtac gcgcagccga cgcaggtcat
     1321 cgcggcgtca aagtcgggca cgaatgcgct ccgcgggagc ctcttcgagt tttaccggtc
     1381 gggcggcatg ggcgcgcgcc ggtgggaggc ggcgagccgc gagtcgttcg tgcggcacca
     1441 gttcggcggc accgtcggcg ggccgatccg gcgcgacaag atgttcttct tcgggtccgc
     1501 cgacgtcttc cggcacacgt cgaacctggt gctcaacgcc cggtacccga cggcggccga
     1561 gcgggccggt gacctttcga gtctgctggc gcgcaccgac gcggcgggcg gcccggcgcc
     1621 gatccgggtc atcgacccgc tcacgggcca gcccttcccg ggcaacgtca tcccggcgag
     1681 ccgcatcgac ccggtcgcgg ccgaactctt gaggctcatc ccggacgcgc cgcggcccgg
     1741 tcgcctgacc gacttcaacg ccgtctacac caagccgcag ttcgacaact cggagaagta
     1801 cgacgcccgg ttcgactaca acgtgacgca gcagcaccgc gtcttcgccc gcgcgaccgt
     1861 cgcccggctc gaccaggcct cgcgctacag cggcgacgtg ccgggcccgc acggctcgag
     1921 cacgaagaag cagtggaact acacggtggc ggccaactgg acgtcggtgc tcagcccgtc
     1981 gacgatcgcg gcgttccagt tcagctaccg caacctgccg ttccgcaaca tcccgtcggt
     2041 gggcgatgag cttttccccg tgccgatcaa cgacgtccgg ccggcgcccc cctacgccgg
     2101 gccgccggcg atcgcgatcg gcagcaacgg gctcggcatc tcgacgctct tcgaccggct
     2161 gctgttcaac gtcagcgagg actacggcta cacgctcgac ccgaacctga cgaaggtcat
     2221 cggcaaccac acgctcaagg ccggcttcac ctacctccgc gggcacaaga cgaccgagat
     2281 cgcctcgccc ccttacggcc ggttcacgac cgtctcggac ttcaacaacg cgcggtcgac
     2341 gacgtcggcc accggcgacg cgttcgcgga cttcctgctc ggctacccgt cgagcaccga
     2401 cgtgaccatc ggcgaggtcg gcggcttcca cagcaagacg aactaccacg cctatctcca
     2461 ggacgactgg aaggtgacgc cgcggctgac gctcaacctc gggctgcggt acgacaactt
     2521 cgggttcttc gaggaagagt tcgggcgcgc
//
LOCUS       DASRIG010000441         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_444776, whole genome shotgun sequence.
ACCESSION   DASRIG010000441 DASRIG010000000
VERSION     DASRIG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444776"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFB52_11750"
     CDS             <1..286
                     /locus_tag="VFB52_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07051.1"
                     /translation="VPADRYVDVVTGRGCAAAGLPETYPRDRAHRRIGWKRCQPIGQA
                     AFQAGAAGIACRSAAPMSPPGGEELAWFQRRRLRRGRTRPFDDWFWPRPA"
     gene            complement(303..>3234)
                     /locus_tag="VFB52_11755"
     CDS             complement(303..>3234)
                     /locus_tag="VFB52_11755"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZO07052.1"
                     /translation="GGIGTVASVPRLLSGQSAIIATGSIAYPPEWAHATPERLRQLGV
                     SKVMTLTSTYDHRVIQGAESGAFLRRLEQLLQGEDGFYEAVVADLGIDAAPITASHPA
                     SASAPPLGTATGAPPAEATTAAPTVDEELLQAVQAATSILKAYRTHGHLAAHLDPLGS
                     EPKGDPALQPENVNLTPELMAKIPAHILRIGVPGETLLEALPRMRTAYCGTIGYQFEH
                     LSSHQQRIWLREMIETGAHRQPLEPDEKRRLLARLIDVFEFERFLEKTYLGQKMFSIE
                     GLDVVVPMLDEAVTLAHRAGAEEVVFGMAHRGRLSVLAHNLGRSVESILAEFEGSKRI
                     ELVKAVAGIPHGGTGDVKYHYGHRGTYETSAGEKIAVRLYPNPSHLEFVDPVVTGGTR
                     YLQSKIDGPKLETDPKLAVPVLLHGDAAFPAQGVVAETLNLQALRGYSTGGTIHLIQN
                     NQVGFTTDPHDSRSTPYAADMAKGFNVPIIHVNADDVEACTAAVRLAMAYRSRWERDI
                     VIDVIGYRRFGHNETDEPAYTQPQMAAKIKSHKPVSEIYSEQLTEEGVVSAEDVKRGS
                     EERKVEMAAALKGLREKMDAGDYEDPTVSTGTGELDRTASPPVDTSLPADQLAKLNEE
                     LLRVPESFTVHRKLRRPLSRRTNALEPGAGGIDFGHGEALALSSLLTEGVHIRMTGQD
                     TERGTFSHRHLVLHDENTGLEYTPMQNLDEAKAPFELHNSPLSEIACLGFEYGYSAAN
                     PSALVIWEAQFGDFANAAQVIIDSFIVSGEAKWGQTSRLTMLLPHGYEGSGPEHSSAR
                     IERFLALGAEGNIRIANPSTAGQYFHLLRHKGAASRLADLTSGTFQFVLEDPRATDRK
                     DQIERLVLCSGKIYYDMDGHERREDAVSVAIARVELLYPFAKEQIRELIASYPNLKEI
                     VWAQEEPQNMGAWKVMTRRMPELVPEKVEFRYVGRPQRASPGEGYPAAHRSEQERIVL
                     TALT"
BASE COUNT          413 a         1083 c         1201 g          537 t
ORIGIN      
        1 ggtcccggcg gaccgctacg tggacgtcgt cactggccgc ggctgtgcgg cggcggggct
       61 gcccgaaacc tatccgcggg atcgggccca ccgacgcatc ggctggaaga gatgccagcc
      121 gatcggccaa gccgccttcc aggcgggcgc cgccgggatc gcctgccgca gcgccgcgcc
      181 gatgtcaccg cccgggggcg aggagctggc ctggttccag cgccgccgcc tgcgccgcgg
      241 ccgcacccgt ccattcgacg actggttctg gcctcgccca gcctagcgac gaagtcgcgg
      301 agttacgtca gagccgtgag cacgatccgc tcctgctcgg agcggtgggc ggccgggtag
      361 ccctcgccgg ggctggcccg ctggggccgg ccgacgtagc ggaactccac cttctccgga
      421 accagctccg gcatgcgccg cgtcatcacc ttccaggcgc ccatgttctg cggctcctcc
      481 tgggcccaga cgatctcctt caggttcgga tagctggcga tcagctcgcg gatctgctcc
      541 ttggcgaagg gatagagcag ctcaacgcgg gcgatcgcca ccgagacggc gtcctcgcgc
      601 cgctcgtggc cgtccatgtc gtagtagatc ttgccgctgc acagcaccag ccgctcgatc
      661 tggtccttgc ggtcggtggc gcgcgggtct tcgagcacga actggaacgt ccccgaggtc
      721 aggtcggcga gccgggacgc cgcccccttg tggcgcagca ggtggaagta ctggccggcg
      781 gtgctggggt tggcgatccg gatgttgccc tcggcgccga gcgccaggaa gcgctcgatc
      841 cgggcgctcg agtgctccgg gcccgagccc tcgtaaccgt gcgggagcag catcgtcagg
      901 cggctggtct ggccccactt cgcctcgccg gagacgatga agctgtcgat gatcacctgc
      961 gcggcgttgg cgaagtcgcc gaactgcgcc tcccagataa cgagcgcgct cgggttcgcg
     1021 gccgagtagc cgtactcgaa gccgaggcag gcgatctccg agagcgggct gttgtgcagc
     1081 tcgaacggcg ccttcgcctc gtccaggttc tgcatcggcg tgtactcgag gccggtgttc
     1141 tcgtcgtgga ggacgaggtg gcggtgggag aaggtgccgc gctcggtgtc ctgaccggtc
     1201 atccggatgt ggacgccctc ggtcagcagc gaggagagcg ccagcgcctc gccgtggccg
     1261 aagtcgatcc cgccggcgcc cggctccagc gcgttggtgc ggcgcgagag cgggcggcgc
     1321 agcttgcggt gcacggtgaa gctctccggc acccgcagca gttcctcgtt cagcttcgcc
     1381 agctggtcgg ccggcagcga ggtatcgacc ggcgggctcg cggtgcggtc gagttcgccg
     1441 gtcccggtgc tcacggtcgg gtcctcgtag tcgcccgcgt ccatcttctc gcgcaggcct
     1501 ttcagcgccg ccgccatctc gactttgcgc tcctcggagc cgcgtttgac gtcctcggcg
     1561 gagaccacac cctcctcggt cagctgctcg gagtagatct cggagaccgg cttgtgcgac
     1621 ttgatcttgg ccgccatctg cggctgcgtg taggcgggct cgtcggtctc gttgtggccg
     1681 aagcggcggt agccgatcac gtcgatcacg atgtcccgtt cccagcgcga gcggtacgcc
     1741 atcgccaggc gcacggccgc tgtacaggcc tcgacgtcgt cggcgttgac gtggatgatc
     1801 gggacgttga agcccttggc catgtcggcc gcgtaggggg tcgagcgcga gtcgtgcggg
     1861 tcggtggtga agccgacctg gttgttctgg atcaggtgga tcgtgccgcc ggtgctgtag
     1921 ccgcgcaggg cctgcaggtt gagcgtctcg gcgaccacgc cctgggcggg gaaggcggcg
     1981 tcgccgtgca gcagcaccgg caccgccagc ttcggatcgg tctccagctt cgggccgtcg
     2041 atcttcgact gcaggtagcg ggtgccgccg gtgaccaccg ggtcgacgaa ctcgaggtgg
     2101 ctcgggttcg ggtacagccg cacggcgatc ttctcgccgg cgctggtctc gtaggtgccg
     2161 cggtggccgt agtggtactt gacgtcgccg gtgccgccgt gcgggatccc ggcgaccgcc
     2221 ttgaccagct cgatccgctt cgagccctcg aactcggcca ggatcgactc gaccgagcgg
     2281 cccaggttgt gggcaagcac gctgagccgg ccgcggtggg ccatcccgaa gacgacctcc
     2341 tcggcgccgg cccggtgcgc cagcgtgacc gcctcgtcga gcatcggcac gaccacgtcg
     2401 agcccctcga tcgagaacat cttctggccc aggtaggtct tctcgaggaa gcgctcgaac
     2461 tcgaagacgt cgatcaggcg ggcgagcagg cggcgttttt cgtcgggctc gagcggttgg
     2521 cggtgggcgc cggtctcgat catctcgcgc agccagatcc gctgctggtg cgaggagagg
     2581 tgctcgaact ggtagccgat cgtgccgcag taggcggtcc gcatccgcgg cagcgcctcc
     2641 agcagcgttt ccccgggcac cccgatgcgg aggatgtggg cggggatctt cgccatcagc
     2701 tccggggtca ggttgacgtt ctcgggctgc agcgccgggt cgcctttcgg ctcggacccg
     2761 agcgggtcga ggtgggcggc gaggtggccg tgggtgcggt aggccttgag gatcgaggtc
     2821 gccgcctgca ccgcctgcag cagttcctcg tcgacggtcg gcgcggccgt ggtcgcttcc
     2881 gcgggcgggg cgccggtggc ggtgccgagc ggcggggccg aggcggaggc ggggtgggag
     2941 gcggtgatcg gtgcggcgtc gatcccgagg tcggcgacga ccgcctcgta gaagccgtcc
     3001 tcgccctgta ggagctgctc gagccggcgc aggaaggcgc ccgactcggc cccctggatc
     3061 acccggtggt cgtaggtcga ggtcagcgtc atcactttcg agacgccgag ctggcgcagc
     3121 cgctccgggg tcgcgtgggc ccactcgggc gggtaggcga tcgagccggt ggcgatgatc
     3181 gcgctctggc cggagagcag ccgcggcacc gaggcgacgg tgccgatccc gccc
//
LOCUS       DASRIG010000442         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_973407, whole genome shotgun sequence.
ACCESSION   DASRIG010000442 DASRIG010000000
VERSION     DASRIG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973407"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1505)
                     /locus_tag="VFB52_11760"
     CDS             complement(12..1505)
                     /locus_tag="VFB52_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZO07053.1"
                     /translation="MERIEHWVVVMFENRSFDSLLGYLPHIDAADGLRDREVVLPYPG
                     GEVRVGPDAVPHDPLPDPGEGFGNINAQLWGAYAPESNMGRSPYPLFADVMDAPFNVP
                     AEPRTPTMDGFALDFYGNFIWEKERQPTAEEMATIGRVFTPETAPVINTLAQEYAVFT
                     RWFCEVPTCTFPNRSFYHGATSLGKIDNEAVLNYAWDQEMPNLFGLCEDKGVSWNVYF
                     DPSQKVPDCAINVAGLRHIRMWRERALERERFFADCAAGTLPAYSWVEPKLMFGELDD
                     YHPPTDVRAGEGFLARVYEAVRSSPAWEKTALLILFDEHGGCFDHVPPPAAPVPDDSP
                     GEQGFGFDRFGVRVPAIVVSPYTERGTVVSDQFHTTAVLRTLRERFDLGPALTNRDAA
                     APSLEVAFNRSEPRTDRPAVTPPPYTEPPPLTPEQKQEAFGDAPDAGLLLAKRKEAAR
                     EHVSQIGEATLRNAARLVEHDLDALPATATDARAWLATHTGHLFAEK"
     gene            complement(1519..>2868)
                     /locus_tag="VFB52_11765"
     CDS             complement(1519..>2868)
                     /locus_tag="VFB52_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO07054.1"
                     /translation="LLHFARNGDFGPEGAELLVLERGEGPYVFDTAGRRYVDGLSSLF
                     CSQLGYSYGAEFAEVAAAQLERLAFNTNWATATPVAIELAARLSAIAPTGPDSKVFLT
                     NGGSESVETAWKLVRQFHLANGEPQRTKAIARDVAYHGVTLGALSLTGVDRFKEPFGP
                     PAIPTRHVANTNAYRSGLDEDTLCEALIAELEAAITAEGPENVAAIFAEPVQNAGGCL
                     LPPRGYWPRLTELARRHGILLVADEVITGMGRLGHWFGVSRYGGEPDIVTAAKGLTSA
                     MAPMGAVLVSERVAAHLYEEKRTLLHGITFGGHPVAAAIALRNLELFESEGVLENVLA
                     LEPHLQARMEELLELPIVGDVRGAGFFWAAELVKEGGERFDADERERLLRGFLPGRLL
                     EAGLIARADDRGDSVIQIAPPLICDAAQLDEVVDALAAVLADAGRFSGVGETAATAT"
BASE COUNT          409 a          964 c         1085 g          410 t
ORIGIN      
        1 ttcccgcgag gttacttctc ggcgaagaga tggccggtgt gggtggcgag ccaggcgcgg
       61 gcgtcggtgg cggtcgccgg cagggcgtcg aggtcgtgct cgaccaggcg ggcggcgttg
      121 cggagggtgg cttcgccgat ctgggagacg tgctcgcggg cggcctcctt gcgcttcgcg
      181 agcagcaggc cggcgtcggg ggcgtcgccg aacgcctcct gcttctgctc gggggtgagc
      241 ggcggcggct ccgtgtaagg gggcggcgtg accgcggggc ggtcggtgcg cggctcggag
      301 cggttgaagg cgacctcgag cgagggcgcg gcggcgtcgc ggttggtcag cgccgggccg
      361 aggtcgaagc gctcgcgcag ggtgcgcagg accgcggtgg tgtggaactg gtcgctgacc
      421 acggtcccgc gctccgtata aggggagacg acgatcgccg gcacgcgcac gccgaagcgg
      481 tcgaagccga agccctgctc gccgggcgag tcgtcgggga ccggggcggc cgggggcggc
      541 acgtggtcga agcagccgcc gtgctcgtcg aagaggatca gcagcgccgt cttctcccag
      601 gcgggcgagc tgcgcacggc ctcgtagacc cgggcgagga acccctcccc cgcgcgcacg
      661 tcggtcggcg ggtggtagtc gtcgagctcg ccgaacatca gcttcggctc cacccaggag
      721 taggcgggca gggtgccggc ggcgcagtcg gcgaagaagc gctcgcgctc cagcgcgcgc
      781 tcacgccaca tccggatgtg gcgcaggccg gcgacgttga tcgcgcagtc gggcaccttc
      841 tggctcgggt cgaagtagac gttccaggag acgcccttgt cctcgcagag cccgaacagg
      901 ttcggcatct cctggtccca ggcgtagttg agcaccgcct cgttgtcgat cttcccgagg
      961 ctggtcgccc cgtggtagaa gctgcggttc gggaacgtgc aggtcggcac ctcgcagaac
     1021 cagcgggtga agaccgcgta ctcctgggcc agggtgttga ttaccggcgc cgtctccggg
     1081 gtgaagacgc gcccgatcgt cgccatctcc tcggccgtcg gctggcgctc cttctcccag
     1141 atgaagttgc cgtagaagtc gagcgcgaac ccgtccatgg tcggcgtgcg cggctcggcc
     1201 gggacgttga acggggcatc catcacgtcc gcgaagagcg ggtagggcga gcgccccatg
     1261 ttcgactccg gcgcgtaggc gccccagagc tgcgcgttga tgttgccgaa gccctcgccg
     1321 gggtctggca gcgggtcgtg cgggaccgcg tcggggccga cccgcacctc gccaccggga
     1381 tagggcagga cgacctcccg gtcacgcaac ccgtcggcgg cgtcgatgtg aggcaggtag
     1441 ccgagcagcg agtcgaacga gcggttctcg aacatgacca cgacccagtg ctcgatccgc
     1501 tccattggag cggggatcct aagtggccgt ggcggcggtc tcgccgactc ccgagaagcg
     1561 cccggcgtcg gccagcacgg cggcgagcgc gtcgaccacc tcgtcgagct gggcggcgtc
     1621 gcagatcagc ggcggcgcga tctggatcac cgagtcaccg cggtcgtcgg cgcgggcgat
     1681 caggcccgcc tccaggaggc gcccgggcag gaagccgcgc aagaggcgct cgcgctcgtc
     1741 ggcatcgaag cgctcgccgc cctccttgac cagctcggcg gcccagaaga aaccggcgcc
     1801 gcgcacgtcg ccgacgatcg gcaactccaa cagctcctcc atccgcgcct gcagatgagg
     1861 ctccagcgcc agcacgttct cgagcacgcc ctcgctttcg aacagctcca ggttgcgcag
     1921 cgcgatcgcc gccgcgaccg ggtggcctcc gaaggtgatc ccgtgcagca gcgtccgctt
     1981 ctcctcatag aggtgagcgg cgacccgctc ggagaccagg accgcgccca tcggcgccat
     2041 cgccgaggtc aggcccttgg cggcggtgac gatgtcgggt tcgccgccgt agcgcgagac
     2101 cccgaaccag tggccgagcc gacccatgcc ggtgatcacc tcgtcggcga ccaggagaat
     2161 tccgtgacgg cgtgcgagct cggtgagtcg cggccagtag ccccgcgggg ggaggaggca
     2221 accaccggcg ttctgcaccg gctcggcgaa gatcgccgcc acgttctcgg ggccctccgc
     2281 cgtgatcgcc gcttccagct cggcgatcag cgcctcgcag agcgtgtcct cgtcgagtcc
     2341 gctgcggtag gcgttggtgt tggcgacgtg ccgggtcgga atcgccggcg ggccgaaggg
     2401 ctccttgaag cggtcgacgc cggtcagcga gagggcgccg agcgtgaccc cgtggtaggc
     2461 gacgtcgcgg gcgatcgcct tcgtccgctg cggttcgccg ttggcgaggt ggaactggcg
     2521 gaccagcttc cacgcggtct cgaccgactc cgagccgccg ttggtgagga agaccttgga
     2581 gtcagggccg gtcggcgcga tcgcactgag ccgcgcggca agctcgatcg cgaccggcgt
     2641 cgccgtcgcc cagttggtgt tgaaggcgag ccgctcgagc tgcgccgccg caacctcggc
     2701 gaactcggcg ccgtaggagt agccgagctg agagcagaag aggctggaga ggccgtcgac
     2761 gtagcggcgg ccggcggtgt cgaagacgta gggcccctcg ccgcgctcca ggaccagcag
     2821 ctccgcgccc tcgggcccga agtcgccgtt gcgggcgaag tgcagcag
//
LOCUS       DASRIG010000443         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1256097, whole genome shotgun sequence.
ACCESSION   DASRIG010000443 DASRIG010000000
VERSION     DASRIG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256097"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFB52_11770"
     CDS             <1..1131
                     /locus_tag="VFB52_11770"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO07055.1"
                     /translation="VNVNRSLDSLRLGEQQLVEIARALLEDPKILVLDEPTSALQKAE
                     TERLLNVVRGLRDRGVAVVYVSHFLEDVFSVADRITVLRSGRVVVARRPREEMTIQGT
                     VAEMLGEAAIREQRHGDATPFEEAAAASAPLVLEDVEVTGSLQPFSLTAEPGEIVGLA
                     GLDGSGAQTVLDVIFGRRKLAGGSITLPGGRRRARTMNQAVRAGIAYVPADRKRLGLM
                     LDSSVAANVASVRGGPLRRMGVVLRNGRMRRRADHWREELKIVMPSSGIPVGSLSGGN
                     QQKVVFAKWLEAEPSVVLLDDPSRGVDMGAKDEMHAIIAQMAASKKVVLYTSSDLEEM
                     AKICDRVVVFFAGRVVGELSSDELSEHRLLEAINVGAVGVAG"
     gene            1195..1899
                     /locus_tag="VFB52_11775"
     CDS             1195..1899
                     /locus_tag="VFB52_11775"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZO07056.1"
                     /translation="MASTEPVRISRAEELSRRLEQEIVDNGLAAGERLGTKEDIRNRF
                     GYAVATVNEALRLLETRGLIEARPGPGGGMFVASPSSRVRLNHLVLGFKVDDAPFTDC
                     LAVRNALEPLVCREAAEHCKAADAKALRQIVARMQKQTGNPKAFLHLNWDLHRRMAKM
                     GSNAPLKTLYLTLLDFVDDGLDQVTGDEFFDAEKNLQVHEELVEAVIDGDLKRLSKAI
                     ERHTPMADRWIGSNGS"
     gene            complement(1962..2753)
                     /locus_tag="VFB52_11780"
     CDS             complement(1962..2753)
                     /locus_tag="VFB52_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07057.1"
                     /translation="MTAREIKRSDKLIALGLALCATVMAVLALAAPANAETYSNGYQL
                     ARFKVEVKGWQKSVQQHHHQAENECDVDDFSSGSESVVFKSKPFVIMASYFPGQHNPE
                     FFAGKRLGIPTTAKIKRSFTPRISFPYPDAQCGENGGGVDPGPGPDCGTRTIKPFDVK
                     LGWGVGKRDEDTLVLNTDYSGEEPYERCPGLAEGGFPFLIVFNAKNDYIGAQISQKEL
                     LDPKFQKWISIANGSRKERSGDYWYKTTLHWEVSFTRLKNKVPGA"
     gene            complement(2787..4346)
                     /locus_tag="VFB52_11785"
     CDS             complement(2787..4346)
                     /locus_tag="VFB52_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO07058.1"
                     /translation="MNRTKTITLELLLASDDALSGRASDEIGSSREFSGRLGLMHTID
                     ELLAQQTTAKENPMNPLDTLPTMDEAAALRRLIGGAAIGPEDANWDSARQAFNLRVDQ
                     QPALIAEPGSREEVATIVRFAREAGLRVAPQRTGHNAEPLGSLSHTILLKTNRLNRVH
                     IDPVARCARVGAGARWEDVVPAASEHGLAALHGSTPDVSVAGYSLGGGLGWYARKHGL
                     AANSITAIEVVTADGAIHLVDADHQPELFWALRGGGGNFGVVTALEIELFPVGEVFAG
                     VLFFPFERANEVLQAWREWTADVPEEVTSVGRLLQFPPLPEIPEPMRGKSFAVIEAVF
                     LGSEAEGAELLAPLRELGPLMDTFAMVPPVGIAELHMDPRDPVPYAGDHTCVGEIDAA
                     AIDELVAAAGPGSGSGLVSVELRHMGGALARAEEGAGVAATMPGSYISYAVGVAADPA
                     TTAQTEADLARVMAALAPYESGRYFNFVERPAAAESFFEPAVAERLRAVKQTYDPTRL
                     FKANHEIGADR"
     gene            complement(4493..>6173)
                     /locus_tag="VFB52_11790"
     CDS             complement(4493..>6173)
                     /locus_tag="VFB52_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZO07059.1"
                     /translation="VAAHFLRVPPLGEEWVAELLIGAGHAAAERGAPDIAVAYLKRAL
                     AESLAPELRTAVLFELGEREMDVRGVDAVPHLREAYEQLTDPREKGWAGYALARTLMF
                     TRREEEAAAVARGAAAELPAGYDDIARALEALEIETIFFGREAADELERTVPYRDGLP
                     GEDVGSKMLLSATAYTWATSGGPSEECAALARQVLADQTLFEMDNGLFWVTALVVLIY
                     ADHDDTSGIWEEVRAVSHRRGSLFMMLTLDLWRGFDMLRRGDLEQAQESIEDALEEML
                     LWAGSEGATVIEWPVGFLGEINVERGDLDAAERILRRGDPGDAVGDGPAFWRKAKLEL
                     MLARGEYEKALEFVDFYEQRLGLMRNPAAAPWRSLKAVALDRLGRTEEAIELVREELE
                     AAQVWGGRQAVGHALRVLGQLRREEGLTDIEAAVETLEQSTARLELAKALLDLGTALR
                     LARQPTKAREPLRRAIEIGSACSADALVERARGELRAAGARPRREALGGIESLTPSER
                     RVAGMAADEMTNREIAQALFVTPKTVEVHLSNAYRKLEISNRRQLATALST"
BASE COUNT          869 a         2150 c         2204 g          950 t
ORIGIN      
        1 gtcaacgtca accgctcgct cgacagcctg cggctgggcg agcagcagct cgtcgagatc
       61 gcccgggcgc tgctcgagga cccgaaaatc ctcgtcctcg acgagccgac ctcggccctg
      121 cagaaagccg agaccgagcg cctgctgaac gtggtccgcg gcctgcgcga ccgcggcgtc
      181 gccgtcgtct acgtctccca cttcctcgag gacgtgttct cggtcgccga ccggatcacg
      241 gtcctgcgct ccggccgcgt cgtcgtcgcc cgccgcccgc gcgaggagat gacgatccag
      301 ggcaccgtcg ccgagatgct cggcgaggcc gcgatccgcg agcagcgcca cggggacgcg
      361 accccgttcg aggaagccgc cgccgcgagc gcgccgctgg tgctcgagga cgtcgaggtc
      421 acgggctccc tgcaaccctt cagcctgacc gccgagccgg gagagatcgt cggcctcgcg
      481 ggcctcgacg gctccggcgc gcagaccgtg ctcgacgtga tcttcggccg ccgcaagctt
      541 gccggcggct cgatcaccct gcccggcggc cgccgccgcg cgcgcacgat gaaccaggcg
      601 gtgcgcgccg ggatcgccta cgtgcccgcc gaccgcaagc gcctcgggct gatgctggac
      661 agctcggtcg ccgccaacgt cgcctcggtt cgcggcggcc cgctgcggcg gatgggcgtg
      721 gtcctgcgca acggccgcat gcgccgccgc gccgaccact ggcgcgagga gctgaagatc
      781 gtgatgccga gctccgggat cccggtcggc tcgctctcgg gcggcaacca gcagaaggtc
      841 gtgttcgcga aatggctcga ggccgaaccc tccgtggtcc tgctcgacga cccctcgcgc
      901 ggcgtcgaca tgggcgccaa ggacgagatg cacgcgatca tcgcccagat ggcggcgagc
      961 aagaaggtcg tcctctacac ctcttcagac cttgaggaga tggcgaaaat ctgcgaccgg
     1021 gtggtcgtct tcttcgccgg ccgcgtggtc ggtgagctct ccagcgacga actctccgag
     1081 caccgtctgc tggaggcaat caacgtcgga gccgtcggcg tagccgggta ggcgcgctat
     1141 agactcggtc tcaagaaccg atgcaatcat cagagtcaaa gcgatggggg gatgatggcg
     1201 tcgactgagc cggtccggat ctcaagggcc gaggagctgt cgcgccgcct ggagcaggag
     1261 atcgtcgata acggtctcgc cgctggcgag cgcctgggca cgaaggagga catccgcaac
     1321 cgcttcggtt acgcggtcgc gaccgtcaac gaggcgctgc gtctgctgga gacgcgcggg
     1381 ctgatcgagg cccggccggg ccccggcggc ggcatgttcg tcgcctcgcc ctcctcgcgc
     1441 gtgcgcctca accacctggt gctcggcttc aaggtcgacg acgccccgtt caccgactgc
     1501 ctggccgtgc gcaacgcgct cgagccgctg gtctgccgcg aagccgccga gcactgcaag
     1561 gccgccgatg cgaaagcgct gcgccagatc gtcgcccgga tgcagaagca gaccggcaac
     1621 ccgaaggcct tcctgcacct caactgggac ctgcaccggc ggatggcgaa aatgggctcg
     1681 aacgcgccgc tgaagaccct ctacctgacc ctgctcgact tcgtcgatga cgggctcgac
     1741 caggtcaccg gcgacgagtt cttcgacgcc gagaagaacc tgcaggtgca cgaagagctg
     1801 gtcgaggctg tgatcgacgg tgatctcaag cgcctgtcca aggcgatcga gcggcacacg
     1861 ccgatggcgg accgctggat cggcagcaac ggctcctgat ccaacggccc gcccgggcgc
     1921 tggcggctag cgccgccggt tgaagaggct cgcgaggaac ctcatgctcc cggcaccttg
     1981 ttcttcagcc gggtaaacga gacctcccag tggagcgtcg tcttgtacca gtagtcgccg
     2041 ctccgctctt tgcgtgagcc gttggcgatc gagatccact tctggaactt cgggtccaag
     2101 agctccttct gtgagatctg ggcgccgatg tagtcgttct tggcgttgaa cacgatcagg
     2161 aacgggaagc cgccttcggc gaggccgggg cagcgttcgt agggttcctc gccgctgtag
     2221 tcggtgttga gcacgagcgt gtcctcgtcg cgcttgccga cgccccagcc gagcttgacg
     2281 tcaaacggct tgatcgtccg cgtgccgcag tcgggcccgg ggccgggatc gacgccgccg
     2341 ccgttttcgc cgcattgcgc gtccgggtag gggaagctga tccgcggcgt gaagctgcgt
     2401 ttgatcttcg ccgtggtcgg gatcccgagg cgtttcccgg cgaagaattc ggggttgtgc
     2461 tggccgggga agtagctggc catgatcacg aacggcttcg acttgaagac gacgctctcc
     2521 gagccgctgg agaagtcgtc gacgtcgcat tcgttttcgg cctggtgatg gtgctgctgg
     2581 accgacttct gccagccctt cacctcgacc ttgaagcggg ccagttggta gccgttgctg
     2641 taggtctcgg cgttcgccgg tgcggccagg gccagcaccg ccatcaccgt cgcgcagagg
     2701 gccagcccca gcgcgatcag cttgtcgctc ctcttgatct ccctcgcagt catctctttt
     2761 gctcctttcc ggttacggag ccgacctcag cggtcggctc cgatctcgtg gttggccttg
     2821 aacaggcggg tggggtcgta ggtctgcttg accgcgcgca ggcgctcggc cacggcgggc
     2881 tcgaagaacg actcggcggc tgccgggcgc tcgacgaagt tgaagtagcg cccgctctca
     2941 tacggggcca gcgccgccat cacccgggcc aggtcggctt cggtctgggc ggtggtggcc
     3001 gggtcggcgg cgacgccgac cgcgtagctg atgtagctgc ccggcatcgt cgcggcgacg
     3061 ccggcgccct cctcggcgcg ggccagggcc ccgcccatgt ggcgcagctc gaccgagacc
     3121 aggcccgagc ccgagccggg cccggcggcc gcgaccagct cgtcgatcgc cgccgcgtcg
     3181 atctcgccga cgcaggtgtg gtcaccggcg taggggacgg gatcgcgcgg gtccatgtgc
     3241 agctcggcga tcccgaccgg tggcaccatc gcgaaggtgt ccatcagcgg acccagctcg
     3301 cgcagcggcg cgagcagctc ggccccttcg gcctcgctgc cgaggaagac cgcctcgatc
     3361 acggcgaacg atttgccgcg catcggctcc gggatctccg gcagcggcgg gaactggagc
     3421 aggcggccga ccgaggtgac ctcctcgggc acgtcggcgg tccactcgcg ccaggcctgc
     3481 aggacctcgt tggcgcgctc gaacgggaag aagagcacgc cggcgaagac ctcgccgacc
     3541 gggaacagct cgatctccag cgcggtgacc acgccgaagt tgccgccgcc gccgcgcagc
     3601 gcccagaaca gctcgggctg gtggtcggcg tcgaccaggt ggatcgcgcc gtcggcggtc
     3661 accacctcga tcgcggtgat gctgttggcg gcgaggccgt gcttgcgggc gtaccagccc
     3721 aggccgccgc cgaggctgta gccagccacg ctgacgtcgg gggtggagcc gtgcagggcg
     3781 gccagaccgt gctcggaggc ggcggggacg acgtcctccc agcgggcgcc ggcgccgacc
     3841 cgggcgcaac gggcaaccgg gtcgatgtgg acgcggttga ggcggttggt cttgagcagg
     3901 atcgtgtgcg acagcgagcc gagcggctcg gcgttgtggc cggtccgctg cggggcgacc
     3961 cgcaggcctg cctcgcgggc gaagcggacg atcgtcgcga cttcctcgcg gctgccgggt
     4021 tcggcgatca gcgccggctg ctggtcgacc cgcaggttga aggcctggcg ggcggaatcc
     4081 cagttggcgt cttcggggcc gatcgcggcg ccgccgatca ggcggcgcag ggcagcggcc
     4141 tcgtccatcg tgggcagggt gtcgagcggg ttcatcgggt tctcctttgc agtggtctgc
     4201 tgggcgagca gctcgtcgat cgtgtgcatc aagccgagcc gcccgctgaa ctcgcgggag
     4261 gagccgattt cgtcgctggc gcgcccgctg agggcgtcat cgctggcgag gagcagctcg
     4321 agcgtgatgg tcttggtgcg gttcatgccc ccagattccg ctctggaggc cgtttgcaca
     4381 tcgagggacc cccgaaaccc cacccccaag atctagctag gggaccccta ggtggggttg
     4441 gggggtgcga gagagatttg tgctcgcgac attgagggcg ccatagcgcc tactaagtcg
     4501 agagggccgt cgccagttgg cggcggttgg agatctcgag cttgcggtag gcgttgctga
     4561 ggtggacctc gaccgtcttc ggggtgacga agagggcctg ggcgatctcg cggttggtca
     4621 tctcgtcggc ggccatgccg gcgacgcggc gctcggaggg ggtgagggat tcgatcccgc
     4681 cgagggcctc gcggcggggg cgggcgccgg cggcgcgcag ctcgccgcgg gcgcgctcga
     4741 ccagggcgtc ggcgctgcag gcgctgccga tctcgatcgc gcggcgcagc ggttcgcgcg
     4801 ccttggtcgg ctggcgggcg aggcgcaggg cggtgccgag gtcgagcagc gccttggcca
     4861 gctccagccg cgcggtcgac tgctccaggg tctcgaccgc cgcttcgatg tcggtgaggc
     4921 cctcctcgcg gcgcagttgg ccgagcaccc gcagggcgtg gccgacggcc tggcgcccgc
     4981 cccagacttg cgccgcctcc agctcctccc ggaccagctc gatcgcctcc tcggtgcggc
     5041 cgagccgatc cagcgccacc gccttcaggc tgcgccacgg ggccgccgcg gggttgcgca
     5101 tcaggcccag tcgctgctca tagaagtcga cgaattccag ggctttctcg tattccccgc
     5161 gggccagcat cagctccagc ttggccttgc gccagaaggc gggaccgtcg ccgaccgcgt
     5221 cgccggggtc gccgcgccgg aggatccgct cggccgcgtc gaggtcgccc cgctcgacgt
     5281 tgatctcgcc gaggaagccg accggccact cgatcacggt ggcgccctcc gagcccgccc
     5341 agagcagcat ctcctcgagc gcgtcctcga tcgattcctg cgcctgctcg aggtcgccgc
     5401 gccgcagcat gtcgaagccg cgccagaggt cgagcgtcag catcatgaac agcgagccgc
     5461 ggcggtggga gacggcccgc acctcctccc agatcccgct cgtgtcgtcg tggtcggcgt
     5521 agatcaggac gaccagcgcg gtcacccaga agagcccgtt gtccatctcg aacagggtct
     5581 ggtcggcgag cacctggcgg gccagcgcag cgcactcctc ggacggcccg ccggaggtgg
     5641 cccaggtgta ggcggtggcg gagagcagca tcttgctgcc cacgtcctcg ccgggcagcc
     5701 cgtcgcggta gggcacggtg cgctcgagct cgtcggcggc ctcgcgtccg aagaagatcg
     5761 tctcgatctc cagcgcctcc agggcgcggg cgatgtcgtc gtaccccgcg ggcagctcgg
     5821 ccgcggcccc gcgggcgacc gccgccgctt cctcctcgcg ccgcgtgaac atcagcgtgc
     5881 gcgccagcgc gtatcccgcc cagcccttct cgcggggatc ggtgagctgc tcataggcct
     5941 cgcgcaggtg cgggaccgcg tcgacgccgc gcacgtccat ctcgcgctcg ccgagctcga
     6001 acagcaccgc ggtgcggagc tccggcgcca gcgactccgc cagcgcccgc ttcaggtagg
     6061 caaccgcgat gtccggggcg ccgcgctcgg cggccgcgtg cccggccccg atcagcaact
     6121 ccgcgaccca ctcctcgccc agcggcggca cccgcaggaa gtgggcggcg act
//
LOCUS       DASRIG010000444         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2279311, whole genome shotgun sequence.
ACCESSION   DASRIG010000444 DASRIG010000000
VERSION     DASRIG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279311"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFB52_11795"
     CDS             complement(<1..482)
                     /locus_tag="VFB52_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO07060.1"
                     /translation="MSARPGTDDLLRMSELAKASGVPVPTIKHYLREGLLPEPVKTSR
                     NMAYYPPEFVERIQLIKRLQETRFMPLKAIKTVLDEDPERAHAMLDLSDQILDRALAG
                     ERARTSASEVRERYGVPAEVLDRLAEIGVLTPTRTGYDADDLQIIEAIVRFRAGGYDE
                     A"
     gene            complement(479..1717)
                     /locus_tag="VFB52_11800"
     CDS             complement(479..1717)
                     /locus_tag="VFB52_11800"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZO07061.1"
                     /translation="MSGLPLADVKVLDLTRLLPGGFCSLLLADLGAEVLKVEDTGMGD
                     YIRWAPPYYGTDAEQQLGTRSALYLSLNRGKRSVRLDLKSEAGREALLRLVVNHDVVL
                     DGFRPGVMDRLGVGYEKLREANPAVVYCAITGYGQDGPNAQRAGHDMNYLALNGLFGL
                     TGPKDGPPIQSAGQIADLGGGALMAAFGTMAALHHARRTGEGQIVDISMADGALSWLA
                     MVAGRYFCDGEIPERGEGQLSGGAACYLPYEATDGWVTCGALEPKFWAAFCNGVERPD
                     LIEKQFEVPGSAAWEEIAAVFRTKTRAEWAAFNDQHDCCIEPVLDLEEALDSELVRAR
                     EMVVELEQPGIGKVRQLGMPVKLSATPADTSRAAPAFGEHTEQVLAEAGYSAAEIAAM
                     IESGAAAGNSGGGAEGEFRA"
     gene            complement(1714..2136)
                     /locus_tag="VFB52_11805"
     CDS             complement(1714..2136)
                     /locus_tag="VFB52_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZO07062.1"
                     /translation="MAEVSIHRAGVAWEGGEGEDVQAHDVLLGAQTLPSSSALELGGD
                     DVKSNPEMLLVGALSSCHMLWFLSLCRKKGFAVASYEDDAEGVLEGKRFSSATLRPRV
                     RWADDPPEPEAIEALHQRSHELCFIANSVNFPVTVEPS"
     gene            complement(2139..>2464)
                     /locus_tag="VFB52_11810"
     CDS             complement(2139..>2464)
                     /locus_tag="VFB52_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZO07063.1"
                     /translation="VTGGIRPVPRNVNLMADQIPGGPEGQNQWQVIGISRDQWKLLAS
                     SLVLGGNVRAGVEDNLYLPNGEMCRSNGDLIGKARQMAEDIGRRPASVAEARELLGVP
                     RRAEA"
BASE COUNT          326 a          860 c          905 g          373 t
ORIGIN      
        1 gcctcgtcgt agcccccggc ccggaaccgc acgatcgcct cgatgatctg caggtcgtcg
       61 gcgtcgtagc ccgtgcgggt cggcgtgagg accccgatct cggcgagccg gtcgaggacc
      121 tcggcgggga cgccgtagcg ctcgcggacc tcgctggcgc tggtccgggc gcgctcgccg
      181 gcgagcgccc ggtcgaggat ctggtctgag aggtcgagca tcgcgtgggc ccgctcgggg
      241 tcctcgtcga gcaccgtctt gatcgccttc agcggcatga agcgcgtctc ctgcaggcgc
      301 ttgatcagct ggatccgctc gacgaactca ggcgggtagt aggccatgtt gcggctggtc
      361 ttgaccggct ccgggagcag gccctcgcgc aggtagtgct tgatcgtcgg aaccggcacg
      421 ccggaggcct tcgccagctc gctcatccgc agcagatcgt cggtgcccgg tcgcgcgctc
      481 acgcgcggaa ctccccctcc gctcctcctc cggagttgcc ggcggccgcg ccggactcga
      541 tcatcgccgc gatctccgcg gccgagtagc cggcctccgc cagcacctgc tcggtgtgct
      601 cgccgaaggc gggcgccgcg cgcgaggtgt ccgcgggggt ggcgctcagc ttcaccggca
      661 tccccagctg gcggaccttg ccgatccccg gctgctccag ctcgaccacc atctcgcgcg
      721 cccgcaccag ctccgagtcg agcgcttcct cgaggtcgag gaccggctcg atgcagcagt
      781 cgtgctggtc gttgaaggcg gcccactcgg cgcgggtctt ggtccggaag accgcggcga
      841 tctcctccca ggccgccgag ccgggtactt cgaactgttt ctcgatcagg tcggggcgct
      901 cgacgccgtt gcagaaggcg gcccagaact tcggctcgag ggcgccgcag gtgacccagc
      961 cgtcggtggc ttcgtagggc aggtaacagg ccgccccgcc ggagagctgg ccctcgccgc
     1021 gctcggggat ctcgccgtcg cagaagtagc ggccggcgac catcgccagc caggagaggg
     1081 cgccatcggc catcgagatg tcgacgatct ggccctcgcc ggtgcggcgc gcgtggtgca
     1141 gcgccgccat cgtcccgaag gcggccatca gggcgccgcc gccgaggtcg gcgatctggc
     1201 cggcggactg gatcggcggg ccgtccttcg gcccggtgag gccgaagagg ccgttcagcg
     1261 ccaggtagtt catgtcgtgg ccggcgcgct gggcgttggg gccgtcctgg ccgtaaccgg
     1321 tgatcgcgca gtagacgacc gcggggttgg cctcgcgtag cttctcgtag ccgaccccga
     1381 gccggtccat cacgccgggg cggaagccgt cgaggacgac gtcgtggttg acgaccaggc
     1441 gaaggagcgc ctcgcggccg gcctcggact tgaggtcgag gcggaccgag cgcttgccgc
     1501 ggttgagcga gaggtagagc gccgagcggg tgccgagctg ctgctcggcg tcggtcccgt
     1561 agtagggcgg cgcccagcgg atgtagtcgc ccatcccggt gtcctcgacc ttcaggacct
     1621 cggcgccgag gtcggcgagc agcagcgagc agaagccgcc gggcagcagc cgggtcaggt
     1681 cgaggacctt gacgtcggcg agcggcaacc cgctcacgag ggctccaccg tgaccgggaa
     1741 gttgaccgag ttggcgatga agcagagctc gtgggagcgc tggtggaggg cttcgatcgc
     1801 ctccggctcg ggcgggtcgt cggcccagcg cacgcgcggg cgcagcgtcg ccgagctgaa
     1861 ccgcttgcct tcgagcacgc cttcggcgtc gtcttcgtaa ctcgcgaccg cgaacccctt
     1921 tttgcggcag agcgagagga accagagcat gtgacagctg gagagggcgc cgaccaggag
     1981 catctcgggg ttcgacttga cgtcgtcgcc gccgagctcc agcgccgagg agctgggcag
     2041 ggtctgggcg ccgagcagga cgtcgtgggc ctgcacgtcc tcgccctcgc ccccctccca
     2101 ggcgacgccg gcgcggtgga tcgagacctc ggccatgact acgcctcggc gcggcgcgga
     2161 accccgagca gctcgcgcgc ctcggcgacg ctcgccggcc ggcggccgat gtcttcggcc
     2221 atctggcggg ccttgccgat caggtccccg ttcgaccggc acatctcgcc gttcggcaag
     2281 tagaggttgt cctcgacccc ggcgcggacg ttgccgccga gcaccagcga ggaggcgagc
     2341 agcttccact ggtcgcgaga gatcccgatc acctgccact ggttctggcc ttcggggccg
     2401 ccggggatct ggtcggccat caggttgacg ttgcgcggga ccgggcggat gccgccggtc
     2461 accc
//
LOCUS       DASRIG010000445         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3875100, whole genome shotgun sequence.
ACCESSION   DASRIG010000445 DASRIG010000000
VERSION     DASRIG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875100"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFB52_11815"
     CDS             complement(<1..342)
                     /locus_tag="VFB52_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HZO07064.1"
                     /translation="MTTDSDPAIAALSELIAELPEELRRQALTHSSWTERRVDSYERL
                     AFLGDSVLGLSVAGGVYERFTSIAAGGLTKIHNQAVSGVSCAAVGRALGVPEMLSEHE
                     PEGVTAAIPASV"
     gene            complement(346..591)
                     /gene="acpP"
                     /locus_tag="VFB52_11820"
     CDS             complement(346..591)
                     /gene="acpP"
                     /locus_tag="VFB52_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZO07065.1"
                     /translation="MNRDQVLTLVREHLAEELEIPIEKISETTRFREDLDADSLDLYE
                     LVMELEDNYGVSVSEEQATRIATVGDAVGFVVEHAPA"
     gene            complement(605..1633)
                     /gene="plsX"
                     /locus_tag="VFB52_11825"
     CDS             complement(605..1633)
                     /gene="plsX"
                     /locus_tag="VFB52_11825"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZO07066.1"
                     /translation="MAPERRVTVALDGYGAEQGFEVLAQGAKLAAADGIGVRVFGPQR
                     ALGLDGVDGIEVIQTAESIGNDEDPVKAVRAKKEASVVRAAADVAAGRSEALVSPGST
                     GATMAAATFGLRRIKGVQRPALAVQLPVPGKPVLFLDVGANVEVRAQHLVQFAFLGAA
                     FSEAVLGVVKPSVGLLTVGEESGKGREEVVEAHRLLSEAENLNFAGNVEGGDLPAAKA
                     DVVVTDGFTGNVALKLMEGTAKTVAAAVGDAARSNPVAAVGGLLLKPALGGLKRELHP
                     DTTGGAILLGLRGIAVVGHGSAGAEGIANAVRLAARCAEVDAVGRTAALLREGGAVRS
                     DFAAGEPA"
     gene            complement(1636..1851)
                     /gene="rpmF"
                     /locus_tag="VFB52_11830"
     CDS             complement(1636..1851)
                     /gene="rpmF"
                     /locus_tag="VFB52_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZO07067.1"
                     /translation="MAVPKKRTSSARRDKRRAQHKAGKARLNSCPRCHSPRLPHRVCP
                     TCGTYAGREVISHQVTDRTVTSASDDE"
     gene            complement(1907..2443)
                     /locus_tag="VFB52_11835"
     CDS             complement(1907..2443)
                     /locus_tag="VFB52_11835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07068.1"
                     /translation="MGQFHWKPETYLEAIRAEVPRYDQLQDAAIAAIPFAPERVLELG
                     MGTGETTRRLIEAHPDSWVIGLDSSPDMVFRAREEYDEVQLARIEDPLPDGPWDLVIG
                     VLSVHHLTTEQKRNLFRRVREHSRSLVVADVVKAEQQVTPIDPTHDFPETAEDLAAWS
                     GGEIVWHADDLAVIRAVY"
     gene            2502..3023
                     /locus_tag="VFB52_11840"
     CDS             2502..3023
                     /locus_tag="VFB52_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZO07069.1"
                     /translation="MPETTTTIDLSSLNLSYGDGKRLDVPVRMAPFELGGQTYLAKPE
                     TVQARLDASRPSGGFAFRLRFPVHLEGPCMRCLEPAALDLEVEAREVDQSDTEDEELR
                     SPYVQDDELEIGRWAHDATMLALPSRLLCRPDCKGLCPVCGESLNDADPADHEHDSGK
                     DPRWAALDKLKLE"
     gene            3088..3294
                     /locus_tag="VFB52_11845"
     CDS             3088..3294
                     /locus_tag="VFB52_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZO07070.1"
                     /translation="MVDFLWKEERLAVQVDGPNRRQRNSDEALADLTLEAAGYRVLHL
                     SEQEVMEEPNRIAELLKRELTRHE"
     gene            complement(3459..3935)
                     /locus_tag="VFB52_11850"
     CDS             complement(3459..3935)
                     /locus_tag="VFB52_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZO07071.1"
                     /translation="MDVLVLIDKLDDVVHNARPVPLTDQVRVDREEIYDLLDQMRATI
                     PEEIKQARWIVKERQEMLAEAKREAERIVREAREQQARLISDEEVTKQAERAADEIVE
                     DARNREREIRLGAEDYADEILNTLEVNLQKFLAAVQRGRDRLAGRDEEPAAELEQP"
     gene            complement(4042..4542)
                     /gene="coaD"
                     /locus_tag="VFB52_11855"
     CDS             complement(4042..4542)
                     /gene="coaD"
                     /locus_tag="VFB52_11855"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZO07072.1"
                     /translation="MSQRNGTVVCPGTYDPVTNGHLDIIDRTSRVFDKVVVGVVNNPL
                     RKQKTLFTAEERKAFIEEATAELANVEVQIFSTLLVEFARANDAKAIVKGMRAISDFE
                     YEFEMAQLNRKLDPGIESIYVIASPHYSFLSSTGVKEMATFGGNVSDLVPDPVASALK
                     ERLPER"
     gene            complement(4539..5084)
                     /locus_tag="VFB52_11860"
     CDS             complement(4539..5084)
                     /locus_tag="VFB52_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HZO07073.1"
                     /translation="MRVIAGELKGQTLVAPRGWKVRPTSDRVREAIFSALGDRVDGAA
                     VLDLYCGTGALAIEALSRGAAHAILVDRDTRPALGNVERLGLRERTELVRNDAARWLE
                     GLGETTTAPTFDLVFVDAPYKLADRVSALLTKHLPALLAADGRAIVESGARRPLRIDS
                     LEPLRPRRYGAADVTIYGAKR"
BASE COUNT          765 a         1769 c         1855 g          812 t
ORIGIN      
        1 gacgctggcg gggatcgccg ccgtgacgcc ctcgggctcg tgctcgctca gcatctccgg
       61 cacgccgagg gcgcggccga ccgccgcgca gctgacgccg ctgaccgcct ggttgtggat
      121 cttggtcagg ccgcccgcgg cgatcgaggt gaagcgctcg tacacgccgc cggcgaccga
      181 gagcccgagc acgctgtcgc cgaggaaggc gaggcgctcg taggagtcga cccggcgctc
      241 ggtccaggaa gagtgggtga gcgcctggcg ccgcagctcc tccggcagct ccgcgatcag
      301 ctccgagagc gccgcgatcg ccgggtcgga gtcggtggtc aaggctcagg cgggcgcgtg
      361 ctcgacgacg aagccgaccg cgtcgccgac cgtcgcgatc cgggtcgcct gctcctcgct
      421 gacgctgacg ccgtagttgt cctcgagctc catcaccagc tcgtagaggt cgagtgagtc
      481 ggcatcgagg tcctcgcgga agcgggtcgt ctcggagatc ttctcgatcg ggatttcgag
      541 ctcctcggcg aggtgctcgc ggacgagggt gagaacttgg tcgcgattca tcccggatgt
      601 cttatcaggc gggttccccg gcggcgaagt ccgagcggac ggcgccgccc tcgcgcagca
      661 gcgccgcggt gcggccgacc gcgtcgacct cggcgcagcg ggcggcgagc cggaccgcgt
      721 tggcgattcc ctcggcgccg gcgctgccgt ggccgaccac ggcgatcccg cgcaggccga
      781 gcaggatcgc gccgccggtg gtgtcggggt gaagctcgcg tttgaggccg cccagggcgg
      841 gtttgagcag cagcccgccg accgcggcga ccgggttgga gcgagcggcg tcgccgaccg
      901 cggcggcgac cgtcttcgcc gtgccctcca tcagcttcaa cgcgacgttg ccggtgaagc
      961 cgtcggtgac gacgacgtcg gccttcgccg ccgggaggtc gccgccctcg acgttgccgg
     1021 cgaagttgag gttctcggcc tcgctcagca ggcggtgcgc ctcgaccact tcctcgcggc
     1081 ccttgcccga ctcctcgccg accgtgagca ggccgacgct gggcttgacg acgccgagca
     1141 cggcctcgct gaaggcggcg ccgaggaagg cgaactggac caggtgctgg gcgcgcacct
     1201 cgacgttggc gccgacgtcg aggaagagca cgggtttgcc cgggaccggc agctggacgg
     1261 cgagcgccgg ccgctgcacg cccttgatcc ggcgcaggcc gaaggtcgcg gccgccatcg
     1321 tcgccccggt cgagccgggg ctgaccagcg cttccgagcg ccccgcggcg acgtcggcgg
     1381 cggcgcgcac gaccgaggcc tctttcttcg cgcggaccgc tttcaccggg tcctcgtcgt
     1441 tgccgatcga ctccgcggtc tggatcacct cgatcccgtc tacgccgtcg aggccgagcg
     1501 cgcgctgggg cccgaagacg cgcacgccga tcccgtcggc cgcggccagc ttcgcgccct
     1561 gcgcgagcac ctcgaagccc tgctcggcgc cgtagccgtc aagggcgacg gtgacccggc
     1621 gctcgggcgc cacggctact cgtcgtcgga agcgctggtc accgtgcggt cggtgacctg
     1681 gtgggagatc acctcacgcc cggcgtaagt cccacaggtg gggcagacgc ggtgcggcag
     1741 ccgcgggctg tgacagcgcg ggcagctgtt gaggcgggcc ttgccggcct tgtgctgggc
     1801 gcgacgcttg tcgcggcggg cactggaagt tctcttcttc gggacagcca ttgcgacgcg
     1861 ggatggtagc ggaagtccga acgcgcgtcg gaatatgcag gcgaaatcag tagacggctc
     1921 ggatcacggc caggtcgtcg gcgtgccaga cgatctcgcc tccgctccaa gctgccaggt
     1981 cctcggcggt ctcggggaag tcgtgggtgg ggtcgatcgg ggtcacctgc tgctcggcct
     2041 tgaccacgtc agcgacgacc agcgagcgcg agtgctcgcg cacgcggcgg aacaggttgc
     2101 gcttctgctc ggtggtcagg tggtgaaccg aaagcacccc gatcaccagg tcccaggggc
     2161 cgtcgggcag cgggtcctcg atccgggcca gctgcacctc gtcgtactcc tcgcgggcgc
     2221 ggaagaccat gtcggggctg gagtcgagcc cgatcaccca ggagtccggg tgcgcctcga
     2281 tcaggcggcg ggtcgtctcc cccgtcccca tccccagctc cagcacccgc tcgggcgcga
     2341 acgggatcgc cgcaatcgcc gcgtcctgga gctggtcgta gcgcggcacc tcggcgcgga
     2401 tcgcctccag gtaagtctcc ggtttccagt ggaattggcc catttcgtaa tctcgtcgtg
     2461 agggtgcccc atcgtaaatc ccccgccagc ggaggtccgc agtgcccgag acgaccacga
     2521 ccatagacct gtccagcctc aacctctcct acggagacgg caagcgcctg gacgtgccgg
     2581 tgcggatggc gccgttcgag ctcggcggtc agacctacct ggcgaagccg gagacggtgc
     2641 aggcgcggtt ggatgcctcg cggccgagcg gcggcttcgc cttccggctg cgcttcccgg
     2701 tccacctcga gggcccctgc atgcgttgcc tggagcccgc cgccctcgac ctcgaggtcg
     2761 aggcgcgcga ggtcgaccag tccgacaccg aggacgagga gctgcgcagc ccctacgtgc
     2821 aggacgacga gctcgagatc ggccgctggg cccatgacgc gaccatgctc gccctcccca
     2881 gccgcctgct ctgccgcccc gactgcaaag gcctttgccc cgtctgcggc gagtccctca
     2941 acgacgccga cccggcggac cacgaacacg acagcggcaa ggacccccgc tgggcggccc
     3001 tcgacaagct caagctggag tagggggaga accgaccgcc gccgggacct tgcgtaggga
     3061 atgcccgggg ctcgagacat gggggtgatg gtcgatttcc tctggaagga ggagcggctc
     3121 gccgtccaag tcgatgggcc gaaccgcagg cagcgaaact ccgacgaagc actcgcggac
     3181 ctgacactcg aggccgccgg ctaccgcgtc ctgcatctca gcgaacaaga ggtgatggag
     3241 gaaccgaacc ggatcgccga gctgctcaag cgagaactga cccgccacga gtaattcagc
     3301 ttccgccagg agatcgggca gcagccccgg ctcgtcggga gggtccgggt gaccctccga
     3361 acgttttccg caactttgcc gccacggcag cacggacgac tgctagacgc agctcaaagg
     3421 atgcgagaga ggagggtcac ccggaccctc ccgcccaact agggctgctc gagctccgcc
     3481 gccggctctt cgtcgcggcc ggcgaggcgg tcgcggccgc gttgcacggc ggcgaggaac
     3541 ttctgcaggt tgacttcgag ggtgttgagg atctcgtcgg cgtagtcctc ggcgccgagg
     3601 cggatttcgc gctcgcggtt gcgggcgtcc tcgacgatct cgtcggcggc gcgctcggcc
     3661 tgcttggtga cctcctcgtc ggagatcagg cgggcctgct gctcgcgggc ctcgcggacg
     3721 atccgctccg cctcgcgttt ggcctcggcc agcatctcct ggcgctcctt gacgatccac
     3781 cgcgcctgct tgatctcctc ggggatcgtg gcacgcatct gatcgaggag gtcatagatc
     3841 tcctcgcgat ccacgcgtac ctgatccgtc agagggacgg ggcgggcgtt gtggacgacg
     3901 tcgtccagct tgtctatgag gaccagtacg tccattggaa gatcgtaagc ctacgtgtac
     3961 gcggggattt tggtgaagat cgcgatatcc agggcgtttt ttgcaagagg acgcccacgc
     4021 gccccagacc ggagcaacag cctatcgctc gggcaggcgc tctttgagcg cactcgccac
     4081 ggggtcggga acgaggtcgg aaacgttgcc tccgaaggtc gccatttcct tcacgccggt
     4141 cgaggaaagg aagctgtagt ggggagaagc gatcacgtag atgctctcga tccccgggtc
     4201 gagcttgcgg ttcagctgcg ccatctcgaa ctcgtactcg aagtcggaga tcgcccgcat
     4261 gcccttgacg atcgccttcg cgtcgttggc gcgggcgaac tcgaccagca gggtggagaa
     4321 gatctgcacc tcgacgttgg cgagctcggc ggtcgcctcc tcgatgaacg ccttgcgctc
     4381 ctccgctgtg aacaaggtct tctgcttgcg cagaggattg ttgacgacgc cgacgaccac
     4441 cttgtcgaag acccgcgagg tgcggtcgat gatgtcgagg tggccgttgg tgaccgggtc
     4501 gtaggtgccc gggcagacga cggttccgtt gcgctggctc atcgcttcgc tccgtagatc
     4561 gtcacgtcgg cggcaccgta gcggcgcggg cgcagcggct ccagcgagtc gatccgcagg
     4621 ggccggcggg cgccgctctc gacgatcgcg cgaccgtcgg cggcgagcag cgcagggaga
     4681 tgcttggtca ggagggcgct cacgcggtcg gcgagtttat agggggcatc gacgaagacg
     4741 aggtcgaagg tcggcgcggt tgtggtctcc cccaggccct cgagccagcg cgcggcgtcg
     4801 ttgcggacca gctcggtgcg ctcgcgcagg ccgagccgct cgacgttgcc gagcgccggg
     4861 cgggtgtcgc ggtcgaccaa aatggcgtgg gcggcgccgc gcgagagcgc ctcgatcgcc
     4921 agcgcgccgg tgccgcagta gaggtcgagc acggcggcgc cgtcgacgcg gtcgccgagc
     4981 gccgagaaga tcgcctcccg cacccggtcg gaggtcggcc gcaccttcca ccccctcggc
     5041 gcaaccagcg tctgcccttt cagctctccg gcgatgactc gcattgcgac gaaaactagt
     5101 cggcgttcgt cctttccggg tcatatgccc gggaaaggac gaacgccgat cagagtggga
     5161 tcggatcggc cgcgccggtg ccgaagcggc ggtgggcggc g
//
LOCUS       DASRIG010000446         6394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2536268, whole genome shotgun sequence.
ACCESSION   DASRIG010000446 DASRIG010000000
VERSION     DASRIG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536268"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFB52_11865"
     CDS             <1..357
                     /locus_tag="VFB52_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZO07074.1"
                     /translation="SRLHFVIHGIHHDHPNDRMRLVMPPAVSVPLAALFLFVYTLIFG
                     TPAAYPIFSGFIVGYLFYDYTHYHVHHHTPKTNLGKRLREQHMRHHFQDHRYGFGVSS
                     PLWDVVFRTLPRTRRD"
     gene            455..826
                     /locus_tag="VFB52_11870"
     CDS             455..826
                     /locus_tag="VFB52_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07075.1"
                     /translation="MSPVSKTVDEAAGLIRRRLKELDAERSQLERALSTLTDGREGRR
                     GPGRPGGSATRTTTRRRRRRGGTRSDQAVKLVAANPGISASEMAKKMNIKPNYLYRVM
                     ADLEKQGKVRKDGRAYHPAKG"
     gene            complement(823..1812)
                     /locus_tag="VFB52_11875"
     CDS             complement(823..1812)
                     /locus_tag="VFB52_11875"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZO07076.1"
                     /translation="MAPSPIKITVTGAAGQIGYAILFRIAAGEMLGDDTPVHFKLLEI
                     PDAIKAAEGTAMELDDCAFPLLEGIDIHDEPKAAFDGCNIALLIGARPRGPGMERSDL
                     LEANGGIFKPQGEAINAHADDDVKILVVGNPANTNCLIAMSHAPDVPRERFTAMMRLD
                     HNRSISQLAAKLGVPVSEITRMTSWGNHSTTQYPDLKHARAGDRNAWDAVDDEAWVVN
                     EFIPDVAKRGAAIIEARGASSAASAANAALDHVRDWVRGTPADDWVSMAVCSDGSYDI
                     PEGLISGYPCTCANGEWSIVEGLEIDDFSRERILASAAELSDERKAVSELGLV"
     gene            complement(1812..3029)
                     /locus_tag="VFB52_11880"
     CDS             complement(1812..3029)
                     /locus_tag="VFB52_11880"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZO07077.1"
                     /translation="MAKIKVENPIVELDGDEMTRIIWSFIKDQLILPYVDVELKYFDL
                     SITSRDATDDQITVDAANAIKEHGVGVKCATITPDEARVEEFGLRRMYRSPNGTIRNI
                     LGGVIFREPIVISNIPRLVPGWKKPIIIGRHAFGDQYRATDTRIPGDGKLMLTFTPND
                     GGRPIELEVFDFPAGGGVAMAMYNLDDSIRDFARASLRYGLERKFPVYMSTKNTIMKT
                     YDGRFKDLFQEVFDAEFKDEFEANGLTYEHRLIDDMVAAALKWEGGYVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLMTPDGRTVEAEAAHGTVTRHFRQHQQGKATSTNPIASIF
                     AWTRALAARGRMDDTPEVSLFADTLERVCVETVESGRMTKDLALLVGGDQAFQTTEEF
                     LASIDENLEREMK"
     gene            3041..3163
                     /locus_tag="VFB52_11885"
     CDS             3041..3163
                     /locus_tag="VFB52_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07078.1"
                     /translation="MLSAQGARPPAGGSIAEQPELVHLRELLETLAANAAEPAG"
     gene            3182..3979
                     /locus_tag="VFB52_11890"
     CDS             3182..3979
                     /locus_tag="VFB52_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO07079.1"
                     /translation="MDAERVRIEVADHVAEVRMVRGDKHNGLDWRMLLALNEAVDALH
                     EQPQVRAVVLCGDGPSFCAGLDFASFLAGDRDLSGDIFERPDGELANRAQRVAFGWRQ
                     LPVPVIAGLHGACFGGGLQIALGADVRIAAPDTRVAVMEIRYGLVPDMSLWQTLPRLV
                     RDDVARELAYTGRIVEAGEAGEVGLLTRIEDNPIAAAHALAAEIAGRSPDAIRRIKRL
                     ANEAPMPPAEGLLREEELQRELLGTPNQAAAVQATLAKQPAEFADPA"
     gene            4026..5696
                     /locus_tag="VFB52_11895"
     CDS             4026..5696
                     /locus_tag="VFB52_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07080.1"
                     /translation="MATAERPRSPAATHEVLNQATPLEDYNTYESDRVLVEALRREGG
                     DWAESRVSEAGAFAGSAQAIRWGFEANENPPRLRAHDRFGNRIDEVEFHPAWHHLLSA
                     GVGFGLHALPWREPQPGAHVARAAMFCASQAEAGVGCPISMTYSVIPALRKQPELADE
                     WEPRFTSLHYDGELRPAAEKPGALCGMGMTEKQGGSDVRANTTTATPLNGGGPGAEYE
                     VNGHKWFFSAPMCDAFLVLAQTEGGLSCLLMPRFTPDGDRNPIHIQRLKDKLGNRSNA
                     SSEVEFRGAWARLIGEEGRGVPTIIEMVNHTRLDCCLGAAAGMRSGVANAIHHASQRS
                     AFGKLLIDQPLMRNVLADLAVESEAATIGSLRLARAYDESIAGDEHATQFKRIANAVL
                     KYWLCKRAVPHAGEALECFGGNGYVEESGMPRLYRESPLNSIWEGSGNVQCLDVLRAM
                     VRGPEAIEAFFAEVGEAAGADSRLDDAAAELRAELGDLDAIESRARRLVERMALVLQG
                     SLVVRFADPAVADAFCASRLGRDWGQAFGTLPAGTDFGRIIERHAVFS"
     gene            5700..>6394
                     /locus_tag="VFB52_11900"
     CDS             5700..>6394
                     /locus_tag="VFB52_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07081.1"
                     /translation="MAPSQRIRRHLQSNAIAYVALFVALSGTAIALPGKNKVKKNDIA
                     RGAVVGKAIAADAVKIAKIRDGAVIASKLGPGAVTTPALLDNAVTAPKLAPDSVTRNA
                     IVQGSINGGKIANGAIDSTKVAQGSLLAADFAPGQLSDAFLFADVTGPQNFTIQRPGR
                     VFVTATVSVLCTNLPCNDGYEVVIDGTPVPGAQLVLPAEMTVEQLTLVGLTGPLAAGP
                     HQIELLSTTPSAD"
BASE COUNT          956 a         2277 c         2178 g          983 t
ORIGIN      
        1 agccggctcc acttcgtcat ccacggcatc catcacgacc atcccaacga ccggatgcgg
       61 ctcgtgatgc ccccggcggt cagcgtgccg ctggctgcgc tcttcctgtt cgtctacacg
      121 ctcatcttcg gaacgcccgc cgcctacccg atcttctcgg gcttcatcgt cggctacctc
      181 ttctacgact acacgcacta ccacgtgcac catcacacgc cgaagacgaa cctcggcaag
      241 cgcctgcgcg agcagcacat gcgccaccac ttccaggatc accgctacgg cttcggggtc
      301 tcctcgccgc tctgggatgt ggtcttccgc accctgccgc gcacccgccg cgactgagcg
      361 caccgaacac cccggatcac actgttagag tgggtcagct gttcacacgc tgatttatga
      421 ctcacagtga agtgagtcgg gaagggggca tcggatgtca cctgtatcga agaccgtaga
      481 tgaggccgcc ggcctgattc gccggcgcct caaggagctc gacgccgagc gctcgcagct
      541 cgaacgtgcc ctctccaccc tcaccgacgg ccgcgagggc cgccgggggc cgggccggcc
      601 cggcggctcg gcgacgcgga ccaccacgcg ccgccgccgg cgtcgcggcg gcacccgctc
      661 cgaccaggcg gtgaagctgg tcgccgccaa ccccggcatc tccgcctccg agatggcgaa
      721 gaagatgaac atcaagccca actacctgta ccgggtgatg gccgatctcg agaagcaggg
      781 aaaggtgcgc aaggacgggc gcgcctacca tccggcgaag ggctagacga gtcccagctc
      841 cgagaccgcc ttgcgctcgt cgctgagctc ggcggccgag gcgagaatcc gctcgcgcga
      901 gaagtcgtcg atctcgagcc cctcgacgat cgaccactcc ccgttcgcgc acgtgcacgg
      961 gtagccggag atcagcccct ccgggatgtc gtaggagccg tccgagcaga ccgccatcga
     1021 gacccagtcg tcggccggcg tgccccgcac ccagtcgcga acgtggtcca gggccgcgtt
     1081 ggcggccgag gctgcgctcg aggcgccgcg cgcctcgatg atcgccgcgc cgcgcttggc
     1141 gacgtcgggg atgaactcgt tcaccaccca ggcctcgtcg tcgaccgcgt cccaggcgtt
     1201 gcggtcgccg gcccgggcat gcttgaggtc cgggtactgg gtcgtcgagt ggttgcccca
     1261 gctggtcatc cgggtgatct cgctcaccgg gaccccgagc ttggcggcga gctgtgagat
     1321 cgaccggttg tggtcgaggc gcatcatcgc cgtgaaccgc tcgcgcggca cgtcgggggc
     1381 gtgcgacatc gcgatcaggc agttggtgtt cgccgggttg ccgacgacga ggatcttcac
     1441 gtcgtcgtcg gcgtgggcgt tgatcgcctc cccctgcggc ttgaagatgc cgccgttggc
     1501 ctcgagcagg tcggaccgct ccattcccgg gccgcgcggg cgggccccga tcagcagggc
     1561 gatgttgcag ccgtcgaacg ccgccttcgg ctcgtcgtgg atgtcgatcc cctccagcag
     1621 tgggaaggcg cagtcgtcga gctccatcgc cgtgccctcg gccgccttga tggcgtccgg
     1681 gatctcgagc agcttgaagt ggaccggggt gtcgtcgccg agcatctcgc cggcggcgat
     1741 cctgaacagg atcgcgtagc cgatctgccc ggcggcgccg gtgacggtga tcttgatggg
     1801 gctgggtgcc actacttcat ctcccgctcg aggttctcgt cgatcgaggc caggaactcc
     1861 tccgtggtct gaaatgcctg gtcgccgccg accagcagcg ccaggtcctt ggtcatcctt
     1921 ccgctttcca cggtctcgac gcatacccgt tcgagtgtgt ccgcgaacag cgacacctcg
     1981 ggcgtgtcgt ccatgcgacc gcgcgcggcg agcgcgcggg tccaggcgaa gatcgaggcg
     2041 atcggattgg tggaggtcgc cttgccctgc tggtgctggc ggaagtggcg agtgacggtc
     2101 ccgtgcgcgg cctcggcctc caccgtcctg ccgtcgggcg tcatcagcac gctcgtcatc
     2161 aggccgagcg acccgaaccc ctgggcgacg gtgtcggatt ggacgtcgcc gtcgtagttc
     2221 ttgcaggccc agacgtagcc cccctcccac ttcagcgccg cggccaccat gtcgtcgatc
     2281 agccggtgct catacgtgag cccgttggcc tcgaactcgt ccttgaactc ggcgtcgaac
     2341 acctcctgga acaggtcctt gaagcggccg tcgtaggtct tcatgatcgt gttcttggtc
     2401 gacatgtaga ccgggaactt gcgctccagc ccgtacctga gcgaggcccg ggcgaagtcg
     2461 cggatcgagt cgtcgaggtt gtacatcgcc atcgcgactc cgccgccggc ggggaagtcg
     2521 aagacctcga gctcgatcgg cctgccgccg tcgtttgggg tgaacgtcag catcagcttg
     2581 ccgtcgccgg ggatccgggt gtcggtggct cggtactggt cgccgaacgc gtgacggccg
     2641 atgatgatcg gcttcttcca ccccgggacc agccgcggga tgttcgagat cacgatcggc
     2701 tcgcggaaga tcaccccgcc gaggatgttg cgaatcgtcc cgttcggcga ccggtacatc
     2761 cgccgcaggc cgaactcctc gacccgcgcc tcgtccggag tgatcgtcgc gcacttgaca
     2821 ccgactccgt gctccttgat cgcgttcgcg gcatcgactg tgatctggtc gtcggtcgcg
     2881 tcgcgactcg tgatcgagag gtcgaagtac ttcagctcga cgtcgacgta gggcaggatg
     2941 agctggtcct tgatgaacga ccagatgatc cgtgtcatct cgtcgccgtc gagctcgacg
     3001 atcggattct cgaccttgat cttcgccatc ggggcgggcg atgctatcgg cgcagggcgc
     3061 ccgcccgccc gccggcggct caatcgccga acagcccgag ctggtgcacc tgcgcgagct
     3121 gctcgagacc ctcgccgcca acgccgccga gcccgcgggc tgaggcacta gattgcgggc
     3181 tatggacgcc gagcgggtgc ggatcgaggt ggccgaccac gtcgccgagg tacggatggt
     3241 gcgcggcgac aagcacaacg ggctggactg gcgcatgctt ctcgccctca acgaggcggt
     3301 cgacgcgctg cacgagcagc cgcaggtgcg ggccgtggtg ctatgcggcg acggtccgag
     3361 cttctgcgcc ggcctcgact tcgcaagctt cctcgccggc gaccgcgacc tgtccggcga
     3421 catcttcgag cgcccggacg gcgagctcgc caaccgtgcc cagcgggtcg cgttcggctg
     3481 gcgccagctg ccggtgccgg tgatcgcagg cctgcacggc gcctgcttcg gcggcggcct
     3541 gcagatcgcc ctcggcgccg acgtgcggat cgccgcgccc gacacgcgcg tcgcggtgat
     3601 ggagatccgg tacggcctcg tcccggacat gagcctctgg cagacgctgc cgcggctggt
     3661 ccgcgacgac gtggcccgcg agctcgctta caccggccgg atcgtcgagg ccggcgaggc
     3721 gggcgaggtc gggctgctga ccaggatcga ggacaacccg atcgccgcgg cgcacgcctt
     3781 ggcggccgag atcgccggcc gctccccgga cgccatccgg cggatcaagc gactggcgaa
     3841 cgaggcgccg atgccgcccg ccgagggcct gctgcgcgag gaggagctcc agcgcgagct
     3901 gctgggaacg ccgaaccagg cggccgccgt ccaggcgacg ctcgccaagc agccggccga
     3961 gttcgccgac ccggcctgag cgtcagggcc ggtgcggcgt cgggctctct gggacaatgg
     4021 cgccgatggc caccgccgag cgcccgcgca gccccgccgc aacccatgag gtcctcaacc
     4081 aggccacccc gctcgaggac tacaacacct acgagtccga ccgcgtcctg gtcgaggcgc
     4141 tgcggcgcga gggcggcgat tgggccgaat cgcgggtgag cgaggcggga gcgttcgccg
     4201 gctcggcgca ggcgatccgg tgggggttcg aagcgaacga gaacccgccg cggctgcgcg
     4261 cccacgatcg gttcggcaac cggatcgacg aggtcgagtt ccaccccgcc tggcaccacc
     4321 tgctgagcgc cggggtcgga ttcggcctgc acgccctccc atggcgagag ccgcagccgg
     4381 gcgcccacgt cgcccgcgcc gccatgttct gcgcctcgca ggcggaggcc ggggtcggct
     4441 gtccgatctc gatgacctat tcggtcatcc cggcgctgcg aaaacagccg gagctggcgg
     4501 acgaatggga gccgaggttc acctcgctgc actacgacgg cgagctgcga cccgcggcgg
     4561 agaagcccgg ggcgctctgc ggcatgggga tgaccgagaa gcagggcggc tccgacgtgc
     4621 gcgccaacac gaccacggcc acgccgctca acggcggcgg tcccggggcc gagtacgagg
     4681 tcaacgggca caaatggttc ttctcggcgc cgatgtgcga tgcgttcctg gtcctggcac
     4741 agaccgaggg cggcctctcc tgcctgctga tgccccgctt cacacccgac ggcgaccgca
     4801 acccgatcca catccagcgg ctcaaggaca agctcggcaa ccgctcgaac gcctccagcg
     4861 aggtcgagtt ccgcggcgcc tgggcgcggc tcatcggcga ggagggtcgc ggcgtcccga
     4921 cgatcatcga gatggtcaac cacacccgcc tcgactgctg cctcggcgcc gccgccggga
     4981 tgcgctccgg tgtcgccaac gcgatccacc atgcctcgca gcgctcggcg ttcggcaagc
     5041 tcttgatcga ccagccgctg atgcgaaacg tgctcgccga cctggcggtc gagtcggagg
     5101 cggcgacgat cggctccctc cgcctcgcgc gcgcctatga cgaatcgatc gccggcgacg
     5161 agcacgcgac ccagttcaag cggatcgcca acgcggtcct caagtactgg ctctgcaagc
     5221 gcgcggtccc gcacgccggc gaggcgctcg agtgcttcgg cggcaacggc tacgtcgagg
     5281 agtcgggcat gccgcgcctc taccgggagt cgccgctcaa ctcgatctgg gaggggtcgg
     5341 ggaacgtgca gtgcctcgac gtcctgcgcg cgatggtccg cggccccgag gcgatcgagg
     5401 cgttcttcgc cgaggtcggc gaggcggccg gcgccgacag ccggctcgac gacgcggccg
     5461 ccgagctgcg cgccgagctc ggcgatctcg acgcgatcga gtcgcgcgcg cggcggctgg
     5521 tcgagcggat ggccctcgtg ctccagggct cgctggtcgt tcgcttcgcc gacccggcgg
     5581 tcgccgacgc cttctgcgcg tcacggctcg gccgcgactg ggggcaggcg ttcgggaccc
     5641 tgccggccgg caccgacttc gggcgcatca tcgagcgtca cgcagtgttc tcgtaggcga
     5701 tggccccctc ccagcgcatc cgtcgacatc tgcaaagcaa cgccatcgcg tacgtcgcgc
     5761 tgttcgtcgc cctcagcggc accgcgatcg cgctgccggg caagaacaag gtgaagaaga
     5821 acgacatcgc ccgcggggcg gtggtcggca aggcgatcgc ggcggacgct gtgaagatcg
     5881 ccaagatccg cgatggggcg gtgatcgcgt cgaagctcgg cccgggggcc gtaacgacgc
     5941 ccgccctgct cgacaacgcc gtcaccgcac cgaagctcgc ccccgactcg gtcacccgca
     6001 acgcgatcgt ccaggggtcg atcaacggcg gcaagatcgc caacggggcg atcgactcga
     6061 ccaaggtggc gcaaggctcg ctgctcgccg cggacttcgc gcccggacag ctcagcgacg
     6121 cgttcctgtt cgcggacgtc accggcccgc agaacttcac gatccagcgg ccggggcgcg
     6181 tgttcgtcac cgcgacggtc tccgtcctct gcacgaactt gccctgcaac gacgggtacg
     6241 aggtcgtgat cgacggcacc cctgttcccg gggcccagct cgtgctaccg gcggagatga
     6301 ccgtggagca gctgaccctg gtcggtctca ccggcccgct ggccgccggg ccgcaccaga
     6361 tcgagctcct ctcaaccacc ccgagcgccg acga
//
LOCUS       DASRIG010000447         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3075012, whole genome shotgun sequence.
ACCESSION   DASRIG010000447 DASRIG010000000
VERSION     DASRIG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075012"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1794)
                     /locus_tag="VFB52_11905"
     CDS             complement(139..1794)
                     /locus_tag="VFB52_11905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZO07082.1"
                     /translation="MVEPGGWSELSAGDVFAGYRIEQRLGRGGMGILYLAVEPGLERR
                     VALKLIAPEAAEEEIFARRFAEESRIAASIEHPNVVPIYAAGEEDGVPYIAMRYVAGS
                     DLGRRIAREGRLKPEVAVALIAQVANGLDAIHAAGLVHRDVKPANVLLSGEQGSEHAY
                     ITDFGVARNVATNSGLTQTGRFVGTLDYVAPEQISGGTVDARVDVYALGCLLFKLLTG
                     EVPFPREGEAARLYAHLNDPPPAPSLYVPAVGMALDDVVARAMAKQPGDRYPSAGDLG
                     RAAVAALSGAAVAIPERTVATGAAATVEPETIAPTEATKASTPPEIEQPTEPVEPTGE
                     TGRLHPGPPEPLTEPDVKGPKRNPLLIGAALAAIAVIVVLGFVLTSSGGGGDDGTGSK
                     DPTTSTPVDTTEEAGGEKEEPVKKPEVDRLTTPELIAQADAICATAKASYGATKEEFS
                     EPETESGMNLAFAEQLVENSSRQVREMKKLTPPTSDVRLYNQYVSMREKIAEFDREAE
                     AAAREQNPTAYVTAFGKRNALTPEMERVADRIGFEECSQNNAG"
     gene            1942..>2226
                     /locus_tag="VFB52_11910"
     CDS             1942..>2226
                     /locus_tag="VFB52_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07083.1"
                     /translation="MSFSGGFTPHGLPRERPVPVTISVSGRVSTVDGTHPPPLRRIVL
                     ELNRNGHISSVGLPVCRAALLQSTTSKEARTRCPGAAVGQGNFAADLAEAG"
BASE COUNT          303 a          732 c          789 g          402 t
ORIGIN      
        1 cggtgatgtg catcgccgat gccagcacca tgccctccgc gatcccggct tcgtcgacac
       61 aagcctggac gtcctcggtg atccggatca gttcgcggcg gttgggagtg ttgaaggtga
      121 ggtgggtggt ggcggttttc atccggcgtt gttctggctg cattcttcga agccaatcct
      181 gtcggcaacg cgttccattt ccggggtcag agcattgcgt ttgccgaacg ccgtcacgta
      241 agcggtggga ttctgttctc ttgccgcagc ttccgcctcg cggtcgaatt ccgcgatctt
      301 ttccctcatc gacacgtact ggttgtacaa gcgaacgtcg ctcgtcggcg gagtgagctt
      361 cttcatttcc ctcacctgcc tcgaagagtt ctccaccagc tgttcggcga atgcgaggtt
      421 catccccgat tccgtttccg gttccgagaa ttcttctttg gtggcgccgt atgaagcctt
      481 ggcagttgcg cagatcgcat cggcctgggc tatcagttcc ggagtcgtga gtcgatcgac
      541 ttccggtttt ttcaccggtt cttctttttc cccacctgct tcttccgtcg tgtccaccgg
      601 cgtcgaggtc gtcgggtctt tcgacccggt tccgtcgtcg ccccctcccc cgctcgaggt
      661 cagaacgaac ccgagcacga cgatgacggc gatcgcggcc agggcggcgc cgatcagcaa
      721 agggttgcgt ttggggcctt tgacgtcggg ctcggtcagc ggctccggag gcccggggtg
      781 caggcggccg gtctcgccgg tcggctccac gggctcggtc ggctgctcga tctccggcgg
      841 ggtcgatgcc ttggtcgcct cggtcggcgc gatcgtctcc ggttctacgg tcgccgccgc
      901 gccggtcgcg acggtgcgct caggaatcgc caccgcggcg ccgctgaggg cagcgacagc
      961 ggcgcggccg aggtcgccgg cagaggggta gcggtcgccg ggctgcttcg ccatcgcccg
     1021 ggcgacgacg tcgtcgagcg ccatccccac cgcgggcacg tagagcgagg gcgcgggcgg
     1081 cggatcgttg aggtgggcgt agaggcgggc ggcctcgcct tcgcgcggga acggcacctc
     1141 gccggtcaac agtttgaaca gcaggcaacc gagggcgtag acgtcgacgc gggcgtcgac
     1201 ggtgccgccg gagatctgct cgggggcgac gtagtcgagg gtgccgacga agcggccggt
     1261 ctgggtcagg ccggagttgg tggcgacgtt gcgggcgacg ccgaagtcgg tgatgtaggc
     1321 gtgctcgctg ccctgctcgc cgctgagcag gacgttggcc ggcttcacgt cgcggtggac
     1381 gaggccggcg gcgtggatcg cgtcgaggcc gttggcgacc tgggcgatca gcgccaccgc
     1441 gacctcgggc ttgagccggc cctcgcgggc gatccggcgg ccgaggtcgg agccggcgac
     1501 gtagcgcatc gcgatgtagg ggacgccgtc ctcctcaccg gcggcgtaga tcgggaccac
     1561 gttcgggtgc tcgatcgagg cggcgatccg cgactcttcg gcgaagcggc gggcgaagat
     1621 ctcttcttcg gcggcctcgg gggcgatcag cttcagcgcg acgcggcgct ccagtcccgg
     1681 ctcgaccgcc aggtagagga tccccatgcc gccgcggccg aggcgctgct cgatccggta
     1741 gccggcgaag acatcaccgg cggagagctc gctccagcct ccaggctcaa ccatcgctca
     1801 accctagccg cagaaacccc tgcttagatt ggccgaggga ggatgcgcag acgctttggc
     1861 atagcggttg cactggccgc ggtcgcactt tcggcggcct cactggcgag cggcgacgta
     1921 agccagaaag gaaacgtccg ggtgagcttc tcgggcggct tcactccgca cgggctgccg
     1981 cgcgagcgcc cggtgccggt gacgatctcg gtctccggcc gggtctcgac ggtcgacggg
     2041 acccacccgc caccgctgcg ccgaatcgtc ctggagctga accgcaacgg acacatctcc
     2101 agcgtgggat tgcccgtatg ccgggccgcg ctgctgcagt cgacgaccag caaggaggcg
     2161 cgcacgcgct gccccggcgc cgcggtcggc cagggcaact tcgccgccga cctggcggaa
     2221 gccggt
//
LOCUS       DASRIG010000448         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_448065, whole genome shotgun sequence.
ACCESSION   DASRIG010000448 DASRIG010000000
VERSION     DASRIG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448065"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1424
                     /locus_tag="VFB52_11915"
     CDS             144..1424
                     /locus_tag="VFB52_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07084.1"
                     /translation="MIADLGALVARRELLGDFALRQLRSRYKGSVLGFGWNFLIPLLQ
                     LVVFWILFGVLLGTRPRTETGEQSYAVFLFVGLLPWTFFANSLQTGAAAIVSNATIVK
                     KVRLPLQLLPAASVLSSLANFLLSLVVLFVVLSVSSDAFLSKDNLLNVLQANADIGIA
                     ACAGTLVIVAGGIDLSIGAIYALAGVLSAKLAISTGVGAGILAGLAAGAAIGLLNGLA
                     VTYGRVNPLIATLAGAIIVGGAAEVVAGQELLTPEAASFVDVGRNSLLGVKYSIWLFA
                     IAAIVLGVLLSRGRFGRETKVVGANEEAARLSGISVTRVRCLTYVLSGVAAAIAGLLA
                     VSSAGQATSNIGGIPFVLSVVAAIAVGGISLRGGEGAVWRTVVGVLFLGLVSNGLGLL
                     DVDPLYDQFFTGLIILLAVSGEVFARRVGSGESR"
     gene            1507..2457
                     /locus_tag="VFB52_11920"
     CDS             1507..2457
                     /locus_tag="VFB52_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07085.1"
                     /translation="MPLDADTQAFLEKSRSKRGPAPGEMSLEEFRAAVEPFRELGFER
                     EDVARVDDLTIPREGAGDVALRLYRPDAHGPLPVFVWVHGGSWVRVTVDLLDNHFRVM
                     ANRSGCAIAAVDYRLSPESRYPEAIEEVYHAAWWLKREAKPLGLDPDRIGLAGDSSGG
                     NVAAAAAIVDRRRRLVGFAFLALVLPVLDARFESDSWRELGEDYLLTKPQLEWAVEQY
                     APDADRDDPLISPVRAPREALEGIPATRILTGEFDPLKDEGAAFAERLREAGTEVELV
                     EVPGLIHHAMMAPKLLETGRDVVVESAEAAGAGLRGNVRA"
     gene            2444..2923
                     /locus_tag="VFB52_11925"
     CDS             2444..2923
                     /locus_tag="VFB52_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO07086.1"
                     /translation="MSAPSEGGPRVTGLHHVGIIVADLDAAVAFATELLGLEVEKRVS
                     LAAESTEIVFLHCGEVLLELIEISDPEVRSRRARVPGAKAEIEHVALAVDDLDAVASE
                     LAEQGLSFTAGAGKVEATTEPLEVAGTKSLFTSRRTSAGILVQLIEEAGKAASTPAE"
     gene            2996..>3425
                     /locus_tag="VFB52_11930"
     CDS             2996..>3425
                     /locus_tag="VFB52_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO07087.1"
                     /translation="MHPTSEAFTSKDNTREIAWSLGGEPSRLLVHADGTRTGGAVTVA
                     EHYWPAGSAGGLHSHGLEDEGFYLIEGELTVNMPDLGESYEVSPGEFLWHPRLSRHDY
                     AVSEAGPARLLQFLVPGGDLVPGFFADIESGHSLEDVVSWG"
BASE COUNT          447 a         1237 c         1205 g          536 t
ORIGIN      
        1 ggggacccac atcgcggcgg acacgacgct gcaggcgtgg aacaagggtc ctgcgccggc
       61 gaagttcctc gccttcttcg tcaccgccga cggcaagccg ttctcgacgg acgtggaggc
      121 gagcccgtag tcgcaggcac accgtgatcg ccgacctcgg cgcgctcgtc gcgcggcgcg
      181 agctcctcgg cgacttcgcg ctgcgccagc ttcgctcgcg ctacaaggga tcggtcctcg
      241 ggttcggctg gaacttcctc atcccgctgc tccagctcgt cgtcttctgg atccttttcg
      301 gcgtgctgct cggcacgcgt ccgcgcaccg agacggggga gcagtcgtac gcggtcttcc
      361 tcttcgtcgg tctgctgccg tggacgttct tcgcgaactc gctccagacc ggcgccgcgg
      421 cgatcgtctc gaacgcgacg atcgtgaaga aggtgcgact gccgctgcag ctgctccctg
      481 cggcgagcgt gctgtcctcg ctcgcgaact tcctgctcag cctcgtcgtc ctcttcgtcg
      541 tgctcagcgt cagcagcgac gccttcctct ccaaggacaa cctgctgaac gtgctgcagg
      601 ccaacgccga catcgggatc gccgcctgcg ccggcaccct ggtgatcgtc gccggcggca
      661 tcgacctctc gatcggagcg atctacgccc tcgccggggt gctctcggcg aagctggcga
      721 tctcgaccgg ggtcggggcc gggatcctcg ccggcctcgc cgccggcgcg gcaatcgggc
      781 tgctcaacgg cctcgccgtc acctatggcc gggtcaaccc gctgatcgcc accctggccg
      841 gcgcgatcat cgtcggcggt gccgccgaag tggtcgccgg gcaggaactg ctgacccccg
      901 aggcggcttc cttcgtcgac gtcggccgca actccctgct cggcgtcaag tacagcatct
      961 ggctgttcgc gatcgccgcg atcgtgctcg gggtgctgct ctcgcgcggc cgcttcgggc
     1021 gcgaaaccaa ggtggtcgga gcgaatgagg aggcggcccg cctctccggc atctcggtca
     1081 cccgggtgcg ctgcctcacc tatgtactga gcggggtcgc ggcggcgatc gccggcctgc
     1141 tcgccgtctc ctcggccggg caggcgacgt cgaacatcgg cggcatccct ttcgtgctct
     1201 cggtggtcgc ggcgatcgcg gtcggcggga tcagcctgcg cggcggcgag ggagcggtct
     1261 ggcgcacggt ggtcggcgtc ctcttcctcg gcctcgtctc caacgggctc ggcctgctcg
     1321 acgtcgaccc gctctacgac cagttcttca ccgggctgat catcctgctc gcggtcagcg
     1381 gcgaggtctt cgcccgccgg gtcgggagcg gcgagtcgcg atgatcggca ccgggctgat
     1441 accggcgccg gatctggtct ccgccggacg aggtcagccg cggtgcggtc gggtagcgtc
     1501 gtgacgatgc cactcgacgc cgacacccag gccttcctcg agaaatcgcg ctcgaaacgg
     1561 gggccggcgc ccggcgagat gtcgctggag gagttccggg ccgcggtcga gccgttccgc
     1621 gagctcggtt tcgagcgcga ggacgtcgct cgagtcgacg acctgacgat cccccgcgag
     1681 ggcgccgggg acgtggcgct gcgcctctac cggcccgacg cgcacgggcc gctgccggtc
     1741 ttcgtctggg tgcacggagg atcctgggtg cgggtcaccg tcgacctgct cgacaaccac
     1801 ttccgggtga tggccaaccg ctccggctgc gcgatcgccg ccgtcgacta ccggctctcc
     1861 ccggagtcgc ggtacccgga agcgatcgag gaggtctacc acgccgcctg gtggctgaag
     1921 cgggaagcga agccgctcgg cctcgacccg gaccggatcg gcctggccgg cgacagcagc
     1981 ggcggcaacg tcgccgctgc cgctgcgatc gtcgaccggc gccggcgcct ggtcggattc
     2041 gccttccttg cactcgtgct gccggtgctc gacgcgcgct tcgagtcgga ttcctggcgc
     2101 gagctgggcg aggactacct cctgaccaag ccgcagctgg agtgggcggt cgagcagtac
     2161 gctccggacg ccgaccgcga cgatccgctg atctcccctg ttcgcgctcc tcgcgaggcg
     2221 ctcgagggga tcccggcgac ccgcatcctg accggggagt tcgatcccct caaggacgag
     2281 ggcgcggcct tcgccgagcg cttgcgcgag gcggggacgg aggtcgagct ggtcgaggtt
     2341 ccgggcctga tccaccacgc gatgatggct ccgaagctgc tcgagacggg ccgcgacgtg
     2401 gtggtcgaat cggccgaggc ggccggcgcg gggctgcggg gcaatgtccg cgcctagcga
     2461 gggcggcccg cgggtcaccg ggctgcacca cgtcgggatc atcgtcgccg acctcgacgc
     2521 cgccgtcgcc ttcgccaccg agctgctcgg gctggaggtc gagaaacggg tcagcctggc
     2581 cgccgagagc accgagatcg tcttcctgca ctgcggcgag gtgctgctcg agctgatcga
     2641 gatctccgat cccgaggtgc gctcgcggcg cgctcgcgtg cccggcgcca aggccgagat
     2701 cgagcacgtc gcgctggcgg tcgacgacct cgacgcggtc gcctccgagc tggccgagca
     2761 gggcctctcg ttcacggccg gcgcgggcaa ggtcgaggcg acgaccgagc cgctcgaagt
     2821 cgccgggacc aagagcctgt tcacctcgcg gcgcacctcc gccggcatcc tcgtgcagct
     2881 gatcgaggag gccggcaagg ccgcctcgac ccccgccgag taggacgagc ggcggggtag
     2941 aggttcgccc cgtccggacg tggtcgcgcc ggctccccgg ccataccgtg aggccatgca
     3001 tccgaccagc gaagcattca cctccaagga caacacgaga gagatcgcct ggagcctcgg
     3061 cggcgagccg tcgcggctgc tcgtccacgc cgacggcacc cggaccggcg gcgcggtcac
     3121 cgtcgccgag cactactggc cggccggcag cgccggcggc ctgcactcgc acggcctcga
     3181 ggacgagggc ttctacctga tcgagggcga gctgaccgtg aacatgcccg acctcggcga
     3241 gtcctacgag gtcagcccgg gcgagttcct ctggcacccg cggctcagcc gccacgacta
     3301 cgcggtcagc gaggcgggcc ccgcccggct cctgcagttc ctcgttcccg gcggcgacct
     3361 ggttcccggc ttcttcgccg acatcgagag cggccacagc ctcgaggacg tggtcagctg
     3421 gggcg
//
LOCUS       DASRIG010000449         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3875881, whole genome shotgun sequence.
ACCESSION   DASRIG010000449 DASRIG010000000
VERSION     DASRIG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875881"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFB52_11935"
     CDS             <1..482
                     /locus_tag="VFB52_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07088.1"
                     /translation="LEDAAAPASAPEGATLIGPSAEAEELTGAEVRRRAAAEAARQRR
                     EQQGEAGKKEHRGLKRAAKVLAVLVVIGALGYAAWWGNRQVWFLGTDDAGRVALYRGL
                     PYELPFGVKLYDERYATPIQTDSLPPRRQDSVTGHDLRSRDDAVSLIEDIEKTQGVE"
     gene            479..1810
                     /locus_tag="VFB52_11940"
     CDS             479..1810
                     /locus_tag="VFB52_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZO07089.1"
                     /translation="MTSARNRELVALVPVALLLTAGFAAVFAQEEAQVGNASLIYGAY
                     FLAICVATHLYLRIRLPNADPYLFPLVATLTAFGLVMIYRIDDGLARDQANWFVLGLI
                     LFALTIQFLRDYEVLERYRYTIAGAGLLLLLAPRLPGIGQQVNGAYLGVKIGPIAFQP
                     AEFTKIAIVVFLASYLRENREVLIVGAKRILGVTLPPLKHFGPMLVVWGASMFMLVFI
                     RDLGSSLMFFAAFLALLYVATGRFSFVVIGMALFALGAWFIASTVPHVHDRIEIWLDP
                     YVDPQGTGYQILNSIFAQADGGLFGKGFGQSLITIPGTDNALLPAAQTDAIYALIVNE
                     VGLFGACGVIAIYLLIAARGFKIALLASDGFSKLLATGLTAVFSIQAFVIIGGITRVI
                     PLTGVTLPFISYGGSSIVANFVLLALLLLISDRARREAVEPAGRDVALGRA"
     gene            1807..3267
                     /locus_tag="VFB52_11945"
     CDS             1807..3267
                     /locus_tag="VFB52_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZO07090.1"
                     /translation="MNKQIVKLFAFIVVLFGVLVGFTSYWAVYDAKALKEKNANKRPL
                     LEQQQIKRGRILTADGVVIAKSVARGKGDELRYVRRYPEGALYGHPIGYSFVQYGDSE
                     FEAFHNDTLVGEESEFASILDEITGATQEGNDIVTSIDSEAQAIALADLEAAGFGAVV
                     AIEPKSGRVRVMASNKSYDPNRVPDEFEELNENELETPLFDRASQGLYPPGSTFKVVT
                     AAAGLENDVITPETALDAPGTLEVEGNPLSNDFNESFSGITLDTALTNSVNTWFAQVG
                     QEVGGDALFETMRKFGFGSTPPIDLPDEEVSVSGVKEPDGRVLGPGDAVDVGRLSIGQ
                     ERLLASPLQMAMVAAAVANKGELMKPQIWSRVVDPDGRVTEKLDPSTYSEPISAETAE
                     ELTTAMEGVVNEGTGTNARIEGVAVAGKTGTAETPGNKSCGGGEDENQAWFIGFAPAD
                     DPEIAIAASVECTPEFGGDVAAPIFRDVAESLLDGE"
     gene            3333..>4736
                     /gene="pknB"
                     /locus_tag="VFB52_11950"
     CDS             3333..>4736
                     /gene="pknB"
                     /locus_tag="VFB52_11950"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZO07091.1"
                     /translation="MPEISEGSVVDGRYKVDRKVGAGGMADVWLAEDTHLQRRVALKV
                     LHKHFAQDREFVARFQREAESAAGLQHPNVVAVYDRGEVDGLNYIAMQYVEGPTLKEL
                     IDRGLTPEQSVVLVRQILEAARFAHRNGIVHRDLKPQNVIVDAEGQAVVTDFGIARAG
                     VSEITQTGSVMGTPQYFSPEQAQGYDVTSVSDLYSIGVILYEALTGRVPFEGESAVAV
                     AMKQVSQVPQRPSTLNPQVSPALDAVVMRALEKEPGERFQSADAFIAALDQAMKEPGT
                     GRGTAAFAALPPVVAVPEDAEAAEDDEEEQRKKRRRKWLIAALVAILIGALIGFLLTR
                     SSTTEVPNVTGKQLEFAILQLERQGFKVGEEEFVERQAPRNTVLEQDPTAGEADEDCP
                     FLGLFCSKPKVNLTISGGPGSGTVPGVSGLTQEEATERLEAAGFEVAVNRVNSASVEE
                     GLVVNSEPSGGSTATNGS"
BASE COUNT          806 a         1654 c         1580 g          696 t
ORIGIN      
        1 agctcgaaga cgccgccgcg ccggcgagcg cgcccgaggg cgcgaccctg atcggcccga
       61 gcgccgaggc cgaggaactg accggcgccg aggtgcgccg ccgcgccgcc gccgaggcgg
      121 cgcgccagcg gcgcgagcag cagggcgagg ccggcaagaa ggagcaccgg gggctgaagc
      181 gcgccgccaa ggtgctcgcc gtcctggtcg tgatcggggc gctcggctac gccgcctggt
      241 ggggcaaccg ccaggtctgg ttcctcggca ccgatgacgc cgggcgggtc gccctctacc
      301 gcggcctgcc ctacgagctc cccttcggcg tgaagctcta cgacgagcgc tacgccaccc
      361 cgatccagac cgactcgctg ccgccgcgtc gccaggactc cgtcaccggc cacgacctgc
      421 gctcccgcga cgacgccgtc tcgctgatcg aagacatcga aaagacccag ggcgtcgaat
      481 gacctcggcc cggaatcggg agctggtcgc cctcgtcccg gtcgcgctcc tcctcaccgc
      541 cggcttcgcc gccgtcttcg cccaggagga agcgcaggtc ggcaacgcga gcctgatcta
      601 cggcgcctat ttcctcgcga tctgcgtcgc cacccacctc tacctgcgga tccgcctgcc
      661 caacgccgac ccctacctgt tcccgctggt cgccacgctg accgccttcg ggctggtgat
      721 gatctaccgg atcgacgacg ggctggcccg cgaccaggcc aactggttcg tgctcgggct
      781 gatcctcttc gccctcacga tccagttcct gcgcgactac gaggtgctgg agcgctaccg
      841 ctacacgatc gccggcgccg gcctgctgct gctgctggcg ccgcggctgc cggggatcgg
      901 ccagcaggtc aacggcgcct acctcggggt gaagatcggc ccgatcgcct tccagccggc
      961 cgagttcacg aagatcgcga tcgtcgtctt cctcgcctcc tacctgcggg agaaccgcga
     1021 ggtgctgatc gtcggcgcca agcgcatcct cggggtgacg ttgccgccgc tcaagcactt
     1081 cgggccgatg ctggtcgtct ggggcgcctc gatgttcatg ctcgtcttta tccgcgacct
     1141 cggcagctcg ctgatgttct tcgccgcctt cctcgccctg ctctacgtcg ccaccgggcg
     1201 cttctcgttc gtggtgatcg ggatggcgct gttcgcgctc ggcgcctggt tcatcgccag
     1261 caccgtgccc cacgtccacg accggatcga gatctggctc gacccctatg tcgacccgca
     1321 gggaaccggg taccagatcc tcaactcgat cttcgcccag gccgacggcg gcctcttcgg
     1381 caagggcttc ggccagtcgc tgatcacgat ccccggcacc gacaacgcgc tcttgccagc
     1441 ggcccagacc gacgcgatct acgcgctgat cgtcaacgag gtcggcctgt tcggcgcctg
     1501 cggggtgatc gcgatctacc tgctgatcgc cgcccgcggg ttcaagatcg ccctgctggc
     1561 cagcgacggc ttctcgaagc tgctggcgac cgggctgacc gcggtcttct cgatccaggc
     1621 cttcgtgatc atcggcggca taacccgggt gatcccgctg accggcgtga ccctgccgtt
     1681 catcagctac ggcggcagct cgatcgtcgc caacttcgtg ctgctcgcct tgctcctgct
     1741 gatctccgac cgggcccgcc gcgaggcggt cgagccggcc ggccgcgacg tcgcgttggg
     1801 acgagcgtga acaaacagat cgtcaaattg ttcgccttca tcgtcgtcct cttcggcgtc
     1861 ctggtcgggt tcacgtccta ctgggcggtc tacgacgcga aggcgctgaa ggaaaagaac
     1921 gccaacaagc ggccgctgct cgagcagcag cagatcaaac gcgggcgcat cctcaccgcc
     1981 gacggcgtgg tgatcgcgaa gtcggtggcg aggggcaagg gcgacgagct gcgctacgtg
     2041 cgccgctacc ccgagggagc cctctacggg catccgatcg gctacagctt cgtccagtac
     2101 ggcgactccg agttcgaggc cttccacaac gacaccctgg tcggcgagga atcggaattc
     2161 gcctcgatcc tcgacgaaat caccggcgcc acccaggagg gcaacgacat cgtcaccagc
     2221 atcgactccg aagcacaggc gatcgcgctg gcggacctcg aagccgccgg gttcggcgcg
     2281 gtcgtagcga tcgagccgaa gagcggccgg gtgcgggtga tggcctcgaa caagtcgtat
     2341 gacccgaaca gggtgcccga cgaattcgaa gagctgaacg aaaacgaact ggaaacgccg
     2401 ctctttgatc gagcctccca gggcctctac ccacccggtt ctaccttcaa ggtggtgacc
     2461 gccgcggcgg ggctggaaaa cgacgtgatc acccctgaaa ccgcgctcga cgcccccggc
     2521 acgctcgaag tcgaagggaa cccgctctcc aacgacttca acgaatcctt ttccgggatc
     2581 accctggaca cggcgctgac caactcggtc aacacctggt tcgcgcaggt gggccaagag
     2641 gtcggcggcg acgccctgtt cgaaacgatg cgcaagttcg gcttcggctc gaccccgccg
     2701 atcgacctgc ccgacgaaga ggtctccgtc agcggggtaa aggaacccga cgggcgggtc
     2761 ctcggtcccg gcgacgcggt tgatgtcggg cgcctctcga tcggccagga gcggctgctg
     2821 gcctcgccgc tgcagatggc gatggtcgcc gccgcggtcg ccaacaaggg cgagctgatg
     2881 aagccgcaga tctggagccg ggtggtcgat cccgatggcc gggtcaccga aaagctcgac
     2941 ccctccacgt acagcgagcc gatcagcgcc gagaccgcgg aagagctgac cacggcgatg
     3001 gaaggggtcg tcaacgaagg gaccggcacc aacgcgcgga tcgaaggcgt ggcggtggcg
     3061 ggcaagaccg gaaccgcgga aacgcccgga aacaagtcct gcggcggtgg cgaagacgaa
     3121 aatcaggctt ggttcatcgg cttcgcgccg gccgacgatc ccgagatcgc gatcgcggcg
     3181 agcgtcgagt gcacgcccga attcggcggc gacgtcgccg ccccgatctt ccgcgacgtg
     3241 gcggaatcgc tcctggacgg cgaatgagag acatgacaaa tggaactggc ccggcaggtt
     3301 gagacagcgg tggagagaga aggaggagcg gggtgcctga gataagcgag gggagtgtgg
     3361 tcgacggccg ttacaaggtc gaccgcaaag tcggggccgg aggcatggcc gacgtctggc
     3421 tggccgaaga cacccacctg cagcggcggg tggcgctgaa ggtcctgcac aagcacttcg
     3481 cccaggaccg cgagttcgtc gcccgcttcc agcgcgaggc ggagtcggcc gcgggcctgc
     3541 agcacccgaa cgtggtcgcg gtctacgacc gcggcgaggt cgacggcctc aactacatcg
     3601 cgatgcagta cgtcgagggc ccgacgctga aagagctgat cgaccgcggc ctcacccccg
     3661 agcagtcggt cgtgctggtc cgccagatcc tcgaagccgc ccgcttcgcc caccgcaacg
     3721 ggatcgtcca ccgcgacctg aagccccaga acgtgatcgt cgacgccgag ggccaggcgg
     3781 tcgtcaccga cttcgggatc gcccgcgccg gcgtctccga gatcacccag accggctcgg
     3841 tgatgggcac cccgcagtac ttctcgcccg agcaggcgca gggctacgac gtgacctcgg
     3901 tctccgacct ctactcgatc ggcgtgatcc tctacgaggc gctgaccggg cgggtcccgt
     3961 tcgaaggcga aagcgccgtc gcggtggcga tgaaacaggt ctcgcaggtt ccgcagcggc
     4021 cgagcacgct gaacccgcag gtctcgccgg cgctcgacgc ggtggtgatg cgggcgctgg
     4081 agaaggaacc gggcgagcgc ttccagagcg ccgacgcgtt catcgccgcg ctcgaccagg
     4141 cgatgaagga accggggacc ggccgcggca ccgccgcctt cgcggcgctg ccgccggtgg
     4201 tcgcggtccc ggaggacgcc gaggcggccg aggacgacga ggaggagcag cgcaagaagc
     4261 ggcggcgcaa atggctgatc gccgcgctcg tcgcgatcct gatcggcgcg ctgatcgggt
     4321 tcctgctcac ccgcagcagc accaccgagg tcccgaacgt gaccggcaag cagctcgaat
     4381 tcgcgatcct gcagctcgaa cgccagggct tcaaggtcgg cgaagaagag ttcgtcgagc
     4441 gccaggcacc gcgcaacacc gtgctcgagc aggacccgac cgccggcgag gccgacgaag
     4501 actgcccctt cctcggtctc ttctgctcga agccgaaggt caacctgacg atcagcggcg
     4561 gtcccggcag cggcaccgtg ccgggcgtct caggtctgac gcaggaagaa gcgaccgaac
     4621 ggctcgaagc cgcgggcttc gaagtcgccg tcaaccgggt caactcagcg agcgtcgaag
     4681 aaggcctggt cgtcaactcc gaaccctcgg ggggcagcac cgcgaccaac ggctcg
//
LOCUS       DASRIG010000450         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2281180, whole genome shotgun sequence.
ACCESSION   DASRIG010000450 DASRIG010000000
VERSION     DASRIG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281180"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1347)
                     /locus_tag="VFB52_11955"
     CDS             complement(337..1347)
                     /locus_tag="VFB52_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO07092.1"
                     /translation="MSSTRHVVELERFGGPEVMRWAEREAPAPGPDEVVVEVDSLGVN
                     FGDTMVRRGEYRRDQPLDFTPGFEAAGRLATAAEGLDAGQPVAVLNDNGRGYTDLLVV
                     PRKRVFAVKEEIEPNLVAALFIQGTTAWYALHRFGYVTAGEWVLVHAGAGGVGSLAIQ
                     LAVAAGARPIATASTPEKLEIARGHGAEHAFLSDPKTLAGEIREATDGHGVDVVVDGV
                     NGPLVAPSMKALAFHGRYVVAGAASQSPGVVDIRGLMPRNQSIAGFVTARIADRDPAE
                     PQNALDQVLAAYRRGELRPSFEVMDPAEIVRAHELLEARSHTGKIVLDLAAYPLGSEV
                     GK"
     gene            complement(1344..2378)
                     /locus_tag="VFB52_11960"
     CDS             complement(1344..2378)
                     /locus_tag="VFB52_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07093.1"
                     /translation="MAGGPLTFGIESQGTSVRAMGALAAAADAAGMDAAWAPELYNRS
                     ATISVAEMANRTAHCRVGTAIAYGVGRSPLIMAAEARDLDELSDGRLVLGLGNGTKRM
                     IADWHGQDPEAPALRMEELVPLLRKLWLLHEGPIDHEGRFYRLKFKPTGDVPPPLRER
                     IPIYTAGVNPRMVETAGRVADGLLGHALFTPAHVEAVVLPALEKGAAKTGRELSEVEV
                     ASLVITVVDDADPERARREAAAQLAFYSSAKTYAGLLESCGFGSEGEAIREAFGRGDW
                     DGMVAAVSDKMVDAIALAGTGEEVRAAARRYEGLFDHLILYAPSYGLSAERAEQNANA
                     VIAAMGEKTR"
     gene            complement(2386..3192)
                     /locus_tag="VFB52_11965"
     CDS             complement(2386..3192)
                     /locus_tag="VFB52_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO07094.1"
                     /translation="MSEFSFNTLDVTRPQDGVLLVTLNRPERLNAVTFEMFDELHELC
                     RTVGKDGSLRAVVMTGAGRGFCAGLDLDEAETLTEMSANEMMLGQEHWAGAMEALHAL
                     PQPVIAAVNGAAAGGGLAFALAADIRIASEAARFNAAFVRIGLSAGDIGVSWSLPRIV
                     GLGHASEIMLTGRFVEAEEAKEIGLVNRVVPADQLLDSALETAAQIASNSPFGITMTK
                     RVLMANVDAGSLRNAIEIENRGQTLATRGDDFREALGAFREKRTPKFSGA"
     gene            complement(3189..>3530)
                     /locus_tag="VFB52_11970"
     CDS             complement(3189..>3530)
                     /locus_tag="VFB52_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07095.1"
                     /translation="QLVQDFIATSRAEIDQARLLTLHAAWKLDEVGNRAARKELSITK
                     LVVPTMGLNVVDRAIQVFGGAGVSEDTPLSWMYRYNRMLRIGDGADEVHKQVIARMEL
                     SAAAARAEEEK"
BASE COUNT          469 a         1257 c         1274 g          530 t
ORIGIN      
        1 atcgtcactc ccatcccggc gatcagctcg tcggtacgca gcgcgttgaa catcagctcc
       61 ttgcctcctt gaagtccgat tcgaggctcg gcgcaccatc gagtcgccga tcacgcttca
      121 cgcagccacg gactcgcggg cggcaacccg ctccgccgcg atcgaaggtg gggcgtgaga
      181 tgagccgcgg cttcggttcg gttgggaggg ttcgatgccc ctctcgcaga ggctcaagga
      241 ccacggtccc gcgcccccgc gagcgactca gctgcgcgac ggtctctgag agaggggcat
      301 cgagccctcc ccctccccat tcgaatctcg gccgtctcac ttccccacct ccgagcccag
      361 cgggtatgcc gccaggtcga gcacgatctt gccggtgtgg ctgcgcgctt ctagcagctc
      421 gtgggcgcgg acaatctcgg cggggtccat cacctcgaat gatggccgca gctcgccgcg
      481 gcggtaggcg gcgagcacct ggtcgagcgc gttctggggc tcggccgggt cgcggtcggc
      541 gatccgggcg gtgacgaagc cggcgatcga ctggttgcgc ggcatcaggc cgcggatgtc
      601 gacgacgccc ggcgactggc tggcggcgcc ggcgacgacg tagcggccgt ggaaggcgag
      661 cgccttcatg ctcggcgcga ccagcgggcc gttgaccccg tcgacgacga cgtcgacgcc
      721 gtggccgtcg gtcgcttcac ggatctcccc ggccagcgtc ttcgggtcgg agaggaaggc
      781 gtgctcggcg ccgtggccgc gcgcgatctc gagcttctcc ggggtcgagg cggtggcgat
      841 cgggcgcgcc ccggcggcga ccgcgagctg gatcgccagc gagccgaccc cgccggcccc
      901 ggcgtggacc agcacccact cccccgcggt cacgtagccg aagcggtgga gtgcgtacca
      961 agcggtcgtg ccctggatga acagcgccgc gaccaggttc ggctcgatct cctccttgac
     1021 cgcgaacacg cgcttgcgcg ggactacgag caggtcggtg tagccgcggc cgttgtcgtt
     1081 gagcacggcg accggctggc cggcgtcgag cccctcggcg gccgtggcga ggcggccggc
     1141 ggcctcgaag ccgggggtga agtcgagcgg ctggtcgcgc cggtactcgc cgcggcggac
     1201 catcgtgtcg ccgaagttga ccccgagcga gtcgacttcg acgacgacct cgtcggggcc
     1261 gggcgcgggc gcctcgcgct cggcccagcg catcacctcg ggaccgccga agcgctccag
     1321 ctcgaccacg tggcgggtgg aactcacctg gtcttctccc ccatcgccgc gatcaccgcg
     1381 ttggcgttct gctcggcccg ctcggcactc agcccgtagc tcggcgcgta gaggatcagg
     1441 tggtcgaaga ggccctcgta gcggcgcgcc gcggcgcgca cttcctcgcc ggtgccggcc
     1501 agcgcgatcg cgtcgaccat cttgtcggag acggccgcga ccatcccgtc ccagtcgccg
     1561 cgcccgaacg cctcgcggat cgcctcgccc tcgctgccga agccgcagga ctcgagcagg
     1621 ccggcgtagg tcttggccga ggagtagaag gcgagctggg cggcggcctc gcgccgcgcc
     1681 cgctccgggt cggcgtcgtc gaccacggtg atcaccagcg aggcgacctc gacttcggag
     1741 agctcgcgcc cggtcttcgc cgcgcctttc tccagcgccg gcagcaccac cgcctcgacg
     1801 tgcgcggggg tgaagagggc gtggccgagc aggccgtcgg cgacccggcc ggcggtctcg
     1861 accatgcgcg ggttgacccc ggcggtgtag atcgggatcc gctcgcgcag cggcggcggc
     1921 acgtccccgg tcggcttgaa cttgagccgg tagaagcggc cctcgtggtc gatcggaccc
     1981 tcgtgcagga gccagagctt gcgcagcagc ggcaccagct cctccatccg cagcgccggc
     2041 gcctccgggt cctggccgtg ccagtcggcg atcatccgtt tggtcccgtt gccgagcccg
     2101 agcaccaggc ggccgtcgga gagctcgtcg aggtcgcgcg cctcggccgc catgatcagc
     2161 gggctgcgcc cgaccccgta ggcgatcgcg gtgccgaccc ggcagtgggc ggtccggttg
     2221 gccatctcgg cgaccgagat cgtcgccgag cggttgtaga gctccggcgc ccaggcggcg
     2281 tccatcccag ccgcgtcagc ggccgcggcg agcgcgccca tcgcgcgcac cgaggtgccc
     2341 tgggactcga tcccgaaggt gagcggaccg ccggccatgc tcgacctagg cgccgctgaa
     2401 cttcggcgtg cgcttctcgc ggaaggcgcc gagcgcctcg cggaagtcgt cgccgcgggt
     2461 ggcgagggtc tggccgcggt tctcgatctc gatcgcgttg cggaggctgc cggcgtcgac
     2521 gttcgccatc agcacgcgct tggtcatcgt gatcccgaag ggcgagttgg aagcgatctg
     2581 ggcggccgtc tccagggcgg aatcaagcag ttggtcggcg gggacgaccc ggttgaccag
     2641 cccgatctcc ttcgcctcct cggcctcgac gaagcggccg gtgagcatga tctcggaggc
     2701 gtggccgagg ccgacgatcc gcggcagcga ccaggagacg ccgatgtcgc cggccgagag
     2761 gccgatgcgg acgaaggcgg cgttgaagcg cgcggcctcg gaggcgatgc ggatgtcggc
     2821 ggccagcgcg aaggccagcc cgccgccggc ggcggcgccg ttgacggcgg cgatcaccgg
     2881 ctgcggcagc gcgtgcagcg cctccatcgc cccggcccag tgctcctggc cgagcatcat
     2941 ctcgttggcg ctcatctcgg tgagggtttc ggcctcgtcg aggtcgagcc cggcgcagaa
     3001 gccgcgcccc gcaccggtca tcaccactgc gcgcagcgag ccgtccttgc cgactgtgcg
     3061 gcagagctcg tgcagctcgt cgaacatctc gaaggtgacc gcgttcagcc gctcgggccg
     3121 gttcagggtg accagcagga caccgtcttg cggacgggtg acgtccaggg tgttgaaact
     3181 gaactcgctc acttctcctc ctcggctcgg gcggccgcgg cggacagttc catccgcgcg
     3241 atcacctgct tgtggacctc gtcggcgcca tccccgatcc gcagcatgcg gttgtagcgg
     3301 tacatccagg agagcggcgt gtcttcggag acgccggcgc cgccgaacac ctggatcgcc
     3361 cggtcgacca cgttcaggcc catcgtcggg accaccagct tggtgatcga gagctccttg
     3421 cgggccgccc ggttgccgac ctcgtcgagt ttccaggcgg cgtgcagggt cagtagccgg
     3481 gcctggtcga tttccgcccg cgaggtggcg atgaagtcct ggacgagctg
//
LOCUS       DASRIG010000451         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_740196, whole genome shotgun sequence.
ACCESSION   DASRIG010000451 DASRIG010000000
VERSION     DASRIG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740196"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFB52_11975"
     CDS             complement(<1..846)
                     /locus_tag="VFB52_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HZO07096.1"
                     /translation="MNDNPNPTPSVELRGVTKRFGEFTAVDDLSLTLGSGEFFTLLGP
                     SGCGKTTTLRMIAGFEQPNEGEIRVEGTDVAGLPPYKRPTNTVFQSYALFPHLSVEDN
                     VAFGLKRQKVGKDEIAERVAAELQRVGLAQEARRKPAQLSGGMQQRVALARALVNLPK
                     VLLLDEPLGALDLKLRKGLQVELKRIQREVGITFVYVTHDQEEALTMSDRIAVMNRGK
                     VEQVDNAEEVYERPTTTFVAGFIGVSNLMPGTISAPGMVKLDSGPEVAGNTEPLQAGQ
                     GCAAVV"
     gene            complement(854..2413)
                     /locus_tag="VFB52_11980"
     CDS             complement(854..2413)
                     /locus_tag="VFB52_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZO07097.1"
                     /translation="MESSAVPASTSADTHAGAHHDKGLKADAITFSDGLTIALASTAP
                     AYSLAAVIGSIVVIVGFQAPAALLVSFVPMFFIAAAFYYLNKADQDCGTSFSWVTRAI
                     GPQSGWITGWAICVTGILVIGSLADVAAYSFFDLIGAESLVESKLAVTLLALAIIALM
                     TTICVLGTELSANLQKGLMILQIGPLLLFAGVALFKVFSGDAPPAAVDPSLSWFNPFE
                     ISSGGALTGALLLGVFIYWGWESAVNLNEETEGEATAPGLAGLISTVILLVTYLGVAA
                     ALVAWKGAAGAEKFDDNEAILSSYATGVLGEPLGSLVLLAVVVSALASTQTTILPASR
                     TSLSMARAEAMPSALGEVSKRFFTPVVSTVAIGVLAVAWYLPSKLVSENFLFDSLSAL
                     ALMIAFYYAFTGAACVVYYRRELTKSVKNFLFVGVAPAIGSVLLGFLFVKALVDYADP
                     ENSYTGESWFGIAPPAVIGVGFILLGVVLLALWRRHRKQPFFLRRREVAPAGFLDNPA
                     IEPKPALAEGE"
     gene            2638..4014
                     /locus_tag="VFB52_11985"
     CDS             2638..4014
                     /locus_tag="VFB52_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZO07098.1"
                     /translation="MATASQSVDTGLQQLARRHLWMHFSRMGGYDDQHEIPIISRAEG
                     CYVYDEHGNRYLDGLSGLFCVNAGHGRSEYGEAAARQIEELDFYTLWSYAHPRAIELA
                     ARIAALAPGDLNRVFFTDGGSEAVESALKLARNYHRMHGKGQKHKLIAREVAYHGTTM
                     GALSATGITELRTQFEPMTPGTHHVPNTNVYRWPEEREPTWAADAIEERIVFEGPESV
                     AAVILEPVQNAGGCFVPPDGYFQHVREICDRYDVLLISDEVICAWGRLGHYFGCERFG
                     YQPDIVTVAKALTSAYAPMGAMIASDEVAAPFMEGNASFSHGFTFAGHPVAAAIAMAN
                     LDVFEKEDLCGHVLAKEAEFRGMLETLHDLPIVGDVRGAGFFQAIEMVKDKETKETFD
                     DEESERLLRGFLSAELYKRGLICRADDRGDPVIQLAPPLISDTEQFEEIHDVLRAVLT
                     EAWEQVVR"
     gene            4028..>4323
                     /locus_tag="VFB52_11990"
     CDS             4028..>4323
                     /locus_tag="VFB52_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZO07099.1"
                     /translation="MLTVQSLLDELDLELAAGGKAAASPVRWVHISELADPTPWLSGG
                     ELMLTTGIPLDTDERQREFVRLLADSHLSGLGFGTGFSHEALPEALIDEAEQRD"
BASE COUNT          721 a         1471 c         1463 g          668 t
ORIGIN      
        1 cacgacggcg gcgcagccct ggccggcctg cagcggctcc gtgttgccgg cgacctccgg
       61 gccgctgtcg agcttcacca tcccgggcgc cgagatcgtt cccggcatca ggttggaaac
      121 gccgatgaag ccggcgacga aggtcgtggt cgggcgctcg tagacctcct cggcgttgtc
      181 gacctgttcg accttgccgc ggttcatcac cgcgatccgg tcgctcatcg tcagcgcctc
      241 ctcctggtcg tgggtgacgt agacgaaggt gatcccgacc tcgcgctgga tccgtttcag
      301 ctccacctgc aggcccttgc gcagcttcag gtcgagggcg ccgagcggct cgtcgagcag
      361 cagcaccttc ggcaggttga ccagcgcccg cgccagcgcc acccgctgct gcatgccgcc
      421 ggagagctgc gccggcttgc gccgcgcctc ctgggccagc ccgacccgct gcagctcggc
      481 cgcgacccgt tcggcgatct cgtccttgcc caccttctgg cgcttcagcc cgaaggcgac
      541 gttgtcctcg acgctgaggt gggggaagag cgcgtagctc tggaagacgg tgttggtcgg
      601 gcgcttgtag ggcggcagac cggcgacgtc ggtgccctcg acccggatct cgccctcgtt
      661 cggctgctcg aagccggcga tcatccgtag cgtcgtcgtc ttcccgcagc cactcggccc
      721 gagcagggtg aagaattcgc cgctgccgag agtgaggctg agatcgtcta cggccgtgaa
      781 ctcgccgaat cgcttggtga cgccgcgcag ctcaacgctc ggggtggggt tcgggttgtc
      841 gttcaatcca ggtctactcc ccctctgcca gcgcaggttt tggctcgatc gccgggttgt
      901 cgaggaaccc tgccggcgcg acttcgcgac ggcgcaggaa gaaaggctgc ttgcggtggc
      961 gccgccagag ggcgagcagg accaccccga ggaggatgaa cccgaccccg atcaccgccg
     1021 ggggcgcgat cccgaaccag gattccccgg tgtaggagtt ttcgggatcg gcgtagtcga
     1081 ccagcgcttt gacgaagagg aagccgagca gcacagagcc gatcgccggg gcgacgccga
     1141 cgaagaggaa gttcttcacc gacttggtca gctcgcgccg gtagtagacg acgcaggcgg
     1201 caccggtgaa ggcgtagtag aaggcgatca tcagggccag ggccgagagc gagtcgaaga
     1261 ggaagttttc actgacgagc ttcgagggga ggtaccacgc gaccgccagt accccgatcg
     1321 ccacggtcga cacaaccggg gtaaagaagc gcttgctgac ctcgccgagg gccgatggca
     1381 tcgcctccgc ccgggccatc gagagcgagg tgcgcgaggc cggcaggatc gtcgtctggg
     1441 tcgaggcgag cgcggagacg acgaccgcga gcaggaccag gctgccgagc ggttcgccga
     1501 gcacgccggt cgcgtagctg gagaggatcg cttcgttgtc gtcgaacttt tccgccccgg
     1561 cggcaccttt ccaggcgacc agcgcggcgg cgaccccgag gtaggtgacc agcaggatca
     1621 ccgtgctgat caggcccgcc aggccgggcg cggtcgcctc gccctcggtc tcctcgttga
     1681 ggttgacggc gctctcccag ccccagtaga tgaagacgcc gagcagcagc gccccggtca
     1741 gggcgccgcc gctggagatc tcgaacgggt tgaaccagga gagcgagggg tcgaccgcgg
     1801 cgggtggcgc atcgccggag aagaccttga acagcgccac cccggcgaac agcagcagcg
     1861 gcccgatctg gaggatcatc agccccttct gcaggttggc cgatagttcc gtcccgagca
     1921 cgcagatcgt cgtcatcaag gcgatgatcg cgagcgcgag gagggtcacc gcgagtttgc
     1981 tttcgaccag cgactcggcc ccgatcaggt cgaagaagga gtaggcggcg acgtcggcga
     2041 gcgagccgat cacgagaatc ccggtgacgc agatcgccca gccggtgatc cagcccgact
     2101 gcgggccgat cgcccgggtc acccaggaga agctggtgcc gcagtcctgg tcggctttgt
     2161 tcaggtagta gaaggcggcg gcgatgaaga acatcggcac gaacgagacc agcagcgccg
     2221 cgggcgcctg gaagccgacg atcacgacga tcgagccgat cacggcggcc agcgagtagg
     2281 ccggcgcggt cgaggcgagg gcgatcgtca gcccgtcgct gaaggtgatc gcgtctgctt
     2341 tcagcccctt gtcgtggtgc gcccccgcgt gggtatccgc gctagtgctc gccgggactg
     2401 ctgagctctc catgcgggta accctccttc gtgggccgtg ccgagttacg gcttcgttcc
     2461 gcgacggtaa gcccccgcgc acatcggcgc aagtggcgga tgtcccaaaa gcacaggaag
     2521 tacttgtacc cgatgtataa atgctgcccc gctggttgac ccattgacca cttgcgtata
     2581 cgccatcctg gctctagcgt tgtccagaac gtcgtagcac gagaagagga gagtcgtatg
     2641 gccaccgcgt ctcagtcagt cgacaccggg ctccagcagc tggcccgccg acatctctgg
     2701 atgcacttct cgcggatggg cggctacgac gaccagcacg agatcccgat catctcgcgc
     2761 gccgagggct gttacgtcta cgacgaacac ggcaaccgct acctcgacgg cctttccggc
     2821 ctcttctgcg tcaacgccgg ccacggccgc agcgagtacg gggaggcggc ggcgcgccag
     2881 atcgaagaac tcgacttcta cacgctctgg agctacgccc acccgcgggc gatcgaactg
     2941 gcggcacgga tcgccgcgct cgcgccgggc gacctcaacc gggtcttctt caccgacggt
     3001 ggctcggagg cggtcgagtc ggcgctgaag ctggcccgca actaccaccg gatgcacggc
     3061 aaagggcaga agcacaagct gatcgcccgc gaggtcgcct accacgggac cacgatgggg
     3121 gcgctctcgg cgaccgggat caccgagctg cgcacccagt tcgagccgat gaccccgggc
     3181 acccaccacg tccccaacac caacgtctac cgctggcccg aggagcgcga gccgacctgg
     3241 gccgccgacg cgatcgagga gcggatcgtc ttcgaggggc cggagtcggt cgccgcggtg
     3301 atcctcgagc cggtccagaa cgccggcggc tgctttgtcc cgcccgacgg ctacttccag
     3361 cacgtccgcg agatctgcga ccgctacgac gtgctgctga tctccgacga ggtgatctgc
     3421 gcctggggcc ggctcggcca ctacttcggc tgcgaacggt tcggctacca gccggacatc
     3481 gtcaccgtcg ccaaggcgct gacctcggcc tacgcgccga tgggcgcgat gatcgcctcc
     3541 gacgaggtcg ctgctccgtt catggagggc aacgcctcct tctcccacgg cttcaccttc
     3601 gccggccacc cggtggccgc ggcgatcgcg atggccaacc tcgacgtctt cgagaaggag
     3661 gacctctgcg gccacgtgct cgccaaggag gccgagttcc gcggcatgct ggagacgctg
     3721 cacgacctgc cgatcgtcgg cgacgtgcgc ggggccggct tcttccaggc gatcgagatg
     3781 gtcaaggaca aggaaacgaa ggagaccttc gacgacgagg agtccgagcg cctgctgcgc
     3841 ggcttcctct ccgctgagct ctacaagcgc ggcctgatct gccgcgctga cgaccgcggc
     3901 gacccggtga tccagctggc accgccgctg atctccgaca ccgagcagtt cgaagagatc
     3961 cacgacgtgc tccgcgccgt cctcactgag gcctgggagc aggtagtgcg ctaggcggtt
     4021 tggccacgtg ctgacggtcc agagcctgct cgacgagctc gacctcgaac tggccgcggg
     4081 cggtaaggcg gcggcctcgc cggttcgctg ggtccacatc tccgagctcg ccgacccgac
     4141 cccatggctc tccggcggcg agctgatgct gaccaccggg atcccgctcg acaccgacga
     4201 gcgccagcgt gagttcgtcc gcctgctcgc cgacagccac ctctccgggc tcggcttcgg
     4261 caccgggttc agccacgaag cgctgccgga ggcgctgatc gacgaggccg agcagcgcga
     4321 ctt
//
LOCUS       DASRIG010000452         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3314358, whole genome shotgun sequence.
ACCESSION   DASRIG010000452 DASRIG010000000
VERSION     DASRIG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3314358"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFB52_11995"
     CDS             complement(<1..247)
                     /locus_tag="VFB52_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07100.1"
                     /translation="MKLTSSAVLLVLLLVAGCGGSDSSFAEDYNKAVAPLSKLGQGMG
                     NDASEFNRLARRTEQTHDNLAKLEAPDDAKDEFDLLLA"
     gene            442..3033
                     /locus_tag="VFB52_12000"
     CDS             442..3033
                     /locus_tag="VFB52_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZO07101.1"
                     /translation="MFERFTERARQVVVLAQEEARILKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSDKIRNEVIRMLSGPGGRRSGSGGGGAQGE
                     GKKSSKLLDQFGRNLTRLAEEGKLDPVIGRETEIERIMQILSRRTKNNPVLLGEPGVG
                     KTAVVEGLAARINKGEVPELLKNKQIYTLDLAALVAGSKYRGEFEERLKKVMKEITQR
                     GDIILFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTLDEYRKYLERDSA
                     LERRFAQIRVDQPSPEDTVRILEGLRERYEQHHRVTINDEALAAAAELADRYISDRFL
                     PDKAIDLIDEAGSRMRIKSMSTPPVYRDLEEEIESTRREKEAAIEAQEFEKAANLRDT
                     ERQLTTKKRQLEDEWAEGENGERPVVGEEEIADIVSMWTGIPVFKLTEAETKKLIRME
                     EELHKRVIGQEVAITAVSKAIRRSRAGIKDPKRPAGSFIFLGPSGVGKTELARTLADF
                     LFGDEEAMVRIDMSEYMEKHAVSRLVGSPPGYIGYDEGGQLTEAVRRKPYSVLLLDEI
                     EKAHPDVFNILLQILEDGRLTDAQGRTVDFRNAIVIMTSNIGASEISKNTSIGFTVSD
                     ETGMSYDDMKNRIMGDLKKVFRPEFLNRIDEVIVFHKLAKEEVKEIIDLMIARVRVQV
                     AEHELQLELTDEAKDVLVDKGWDPSMGARPLRRAIQRYIEDPLADEVLRQGEMAPGST
                     VMVTRDASGDEEDKPLRLEIIPPAKPPKSKDKPEPEKVGVGAKQGDEGEHGHDDVPDA
                     PGGDAES"
     gene            3047..4177
                     /locus_tag="VFB52_12005"
     CDS             3047..4177
                     /locus_tag="VFB52_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZO07102.1"
                     /translation="MREPGRSAIGTWSGGRFLHFGEAIEEPRLVELLRPDAGIDTVLT
                     ADAYGQGEADRVLGQALTGVARDSYSLVGAVGHDFYEGERNGPKGFPRFTDPALRKPD
                     QYASYLRMATEASLERIGADSFDLLLLHNPDRTGYSSEVVWDGMATLREVGLTAQIGV
                     APGPANGFTLDILSCLERFGDRIDWAMVILNPLEPWPGELCLDAAERQGVKVLTRVVD
                     YGGLFWGDLTPTTELPRTDHRSFRPAGWIEAGLEKLDRLRPFAERADLTTIQLACQWN
                     LAHPAVASVAPTLIQETGEDARAIEDKRAELAAMPEQRLGESEREAIRAIGDNSGCMA
                     LKGASPEHEGAEQPDRWALDAELAAVGARWGVDPARDLVVTH"
     gene            4207..>4525
                     /locus_tag="VFB52_12010"
     CDS             4207..>4525
                     /locus_tag="VFB52_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO07103.1"
                     /translation="MTRQWWTLAGACAGLFILMLDSTVVALALPEIRGDVGASAEQLQ
                     WVMNVYLLAIAVLVVTAGRLGDMFGRKRAFLVGMVIFALGSVVSATAASAEMLIAGRV
                     IQGI"
BASE COUNT          785 a         1576 c         1517 g          647 t
ORIGIN      
        1 gcgccaagag caggtcgaac tcgtccttgg cgtcgtcggg cgcctcgagc ttcgcgaggt
       61 tgtcgtgggt ctgctcggtc cgccgcgcca ggcgattgaa ctcgctcgcg tcgttcccca
      121 tgccctggcc cagcttgctg agcggcgcca cggccttgtt gtagtcctct gcgaaggagc
      181 tgtccgagcc gccgcagccg gccaccagaa gcaggacgag aagcaccgcc gacgaagtga
      241 gcttcattat ccggaacgta cccacgatgc gggttttagg ccttcgtgaa gggcaaatcc
      301 ggtctttggt gcgttggtag agttgtcgca gcgggtcgtg gttctcgcct acctctcacg
      361 gcgaccggcg tagctgccga tctagtgaga aagaacgggt aaccccgccc ccgaagttcc
      421 cgacagataa gagagcacga agtgtttgag cgcttcaccg aacgggcccg gcaggtggtc
      481 gtcctcgccc aagaggaggc ccgaattctc aagcacaatt acatcggcac tgagcacatc
      541 ctcctcggcc tgctgcgcga ggaggagggc ctcgcagccc gcgtcctcga gtccctcgac
      601 atcaccgtgg agcgcgtgcg cgcccaggtg gtgcgcatcg tcgggtccgg cgaggaggtc
      661 acctccgggc agatcccgtt caccccgcgc gccaagaagg tgctcgagct cgccctgcgc
      721 gaggcgctct cgctcggcca caactacatc ggcaccgagc acatcctgct cggcctcgtc
      781 cgcgagaacg agggcgttgc cgcccgcatc ctgctcgatt tcgatgccga ctcggacaag
      841 atccgcaacg aggtgatccg gatgctctcc ggccccggcg gtcgtcgcag cggcagcggt
      901 ggcggcggcg cccagggcga gggcaaaaag tcttcgaaac tgctcgacca gttcgggcgc
      961 aacctcaccc gcctcgcgga ggagggcaag ctcgacccgg tgatcggtcg cgagaccgag
     1021 atcgagcgga tcatgcagat cctctcccgg cgcaccaaga acaacccggt gctgctcggt
     1081 gagcccggcg tcggcaagac cgccgtggtc gagggcctgg ccgcccggat caacaaaggc
     1141 gaggttccgg agctgctgaa gaacaaacag atctacacgc tggatctggc cgccctggtt
     1201 gccggctcga agtaccgcgg cgagttcgag gagcgtctca agaaggtgat gaaagagatc
     1261 acccagcgcg gcgacatcat tctcttcatc gacgagctcc acaacctggt cggcgccggc
     1321 gccgccgagg gcgcgatcga cgcggcctcg atcctgaagc cggccctggc ccgcggcgag
     1381 ctgcagacga tcggcgcgac cacgctcgac gagtaccgca agtacctcga gcgcgactcg
     1441 gcgctggagc gccgcttcgc ccagatccgc gtcgaccagc cctcgcccga ggacacggtc
     1501 cgcatcctcg agggcctgcg cgagcgctac gaacagcacc accgggtgac gattaacgac
     1561 gaagcgctgg ccgccgcggc cgagctcgcc gaccgctaca tctccgaccg cttcctgccc
     1621 gacaaggcga tcgacctgat cgacgaggcc ggctcgcgca tgcgcatcaa gagcatgagc
     1681 acgccgccgg tctaccgcga cctcgaggag gaaatcgagt cgacgcggcg cgagaaggag
     1741 gccgcgatcg aggcccagga gttcgaaaaa gccgccaacc tgcgcgacac cgagcgtcag
     1801 ctgacgacca aaaagcgcca gctcgaagac gagtgggcgg agggcgagaa cggcgagcgc
     1861 ccggttgtcg gcgaggagga gatcgccgac atcgtctcga tgtggaccgg catcccggtc
     1921 ttcaaactga ccgaggccga gaccaaaaag ctgatccgga tggaggagga actccacaag
     1981 cgggtgatcg gccaggaggt cgcgatcacc gccgtctcga aagcgatccg ccgctcgcgg
     2041 gccgggatca aggacccgaa gcggcccgcc ggctcgttca tcttcctcgg cccctccggg
     2101 gtcggcaaga ccgagctcgc ccgcacgctc gccgatttcc tcttcggcga cgaggaggcg
     2161 atggtccgga tcgacatgtc cgagtacatg gagaagcacg ccgtcagtcg tctggtcggc
     2221 tcgcctcccg gctacatcgg ctacgacgag ggcggtcagc tgaccgaggc cgtgcgccgc
     2281 aagccctact cggtgctgct gctcgacgag atcgagaagg cccaccccga cgttttcaac
     2341 atcctgctgc agatcctcga ggacgggcgc ctcaccgacg cccagggtcg caccgtggac
     2401 ttccgcaacg cgatcgtgat catgacctcg aacatcggcg cttcggagat ctcgaagaac
     2461 acctcgatcg ggttcaccgt ctccgacgag accgggatgt cctacgacga catgaagaac
     2521 cggatcatgg gcgacctgaa aaaagtcttc cggcccgagt tcctcaaccg gatcgacgag
     2581 gtgatcgtct tccacaagct cgcgaaggag gaggtcaagg agatcatcga cctgatgatc
     2641 gcccgcgtcc gcgtccaggt cgccgagcac gaactgcagc tggagctgac cgacgaggcc
     2701 aaggacgtgc tggtcgacaa aggctgggac ccctcgatgg gcgcccgccc gctgcgccgc
     2761 gcgatccagc gctacatcga ggacccgctg gccgacgaag tcctgcgcca gggcgagatg
     2821 gcccccggtt cgaccgtgat ggtcacccgc gacgcctccg gcgacgagga agacaaaccg
     2881 ctgcggctgg aaatcatccc gccggcgaag ccgcccaagt cgaaggacaa gcccgagccc
     2941 gagaaggtcg gggtcggcgc caagcagggc gacgagggcg agcacgggca cgacgacgtc
     3001 ccggacgcgc ccggcggcga cgccgagtcc tgagcctgta gcgcttgtga gagagcccgg
     3061 ccgcagcgcg atcggcacct ggagcggcgg ccgcttcctg cacttcggcg aagcgatcga
     3121 ggagccgcgg ctggtcgagc tgctgcgccc ggatgccggc atcgacacgg tgctgaccgc
     3181 cgacgcctac ggccagggtg aggccgaccg cgtgctcggc caggccctga ccggcgtcgc
     3241 gcgcgactcc tacagcctcg tcggcgccgt cggccacgac ttctacgagg gcgagcgcaa
     3301 cgggccgaag ggcttcccgc gcttcaccga ccccgccctg cgcaagcccg accaatacgc
     3361 gagctacctg cggatggcga ccgaggcgag cctcgaacgg atcggcgccg actccttcga
     3421 cctgcttctc ctccacaacc ccgaccgcac cggctactcc tcggaggtcg tctgggacgg
     3481 gatggcgacg ctgcgcgagg tcggtctcac cgcgcagatc ggtgtcgctc ccggccccgc
     3541 caacggcttc accctcgaca tcctttcctg cctggagcgg ttcggcgacc ggatcgactg
     3601 ggcgatggtg atcctcaacc cgctcgagcc ctggcccggc gagctttgcc tcgacgccgc
     3661 cgagcgccaa ggggtcaagg tgctgacccg ggtggtcgac tacggcggcc tcttctgggg
     3721 cgacctgacg ccgaccaccg agctgccccg caccgaccac cgctccttcc gcccggccgg
     3781 ctggatcgag gcggggctag agaagctcga ccgcctgcgc ccgttcgccg agcgggccga
     3841 cctgaccacg atccagctcg cctgccagtg gaaccttgcc cacccggccg tcgcctcggt
     3901 cgccccgacc ctgatccagg agacgggcga ggacgcgcgc gcgatcgagg acaagcgggc
     3961 cgagctggcg gcaatgcccg agcagcgcct cggcgagtcc gagcgcgagg cgatccgggc
     4021 gatcggcgac aacagcggct gcatggcgct gaagggcgcc agccccgagc acgagggcgc
     4081 cgagcagccc gatcgctggg cgcttgacgc ggagcttgcc gcggtcggtg cccgctgggg
     4141 tgtcgacccc gcccgggatc tggtcgtcac ccactgacgc ccggcgccga cggtaggttc
     4201 ggcgcgatga ctcgccagtg gtggacgctc gcgggcgctt gcgccggtct cttcatcctg
     4261 atgctcgact cgaccgtggt cgccctggcg ctgccggaga tccgcggcga cgtcggcgcc
     4321 agcgccgagc agctgcagtg ggtgatgaac gtctacctgc tggcgatcgc cgtgctcgtc
     4381 gtcaccgccg gccggctcgg cgacatgttc ggccgcaagc gcgccttcct cgtcgggatg
     4441 gtgatattcg ccctcggctc ggtggtctcg gcgaccgcgg cgagcgcgga gatgctgatc
     4501 gccgggcgcg tgatccaggg gatcg
//
LOCUS       DASRIG010000453        22113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2281870, whole genome shotgun sequence.
ACCESSION   DASRIG010000453 DASRIG010000000
VERSION     DASRIG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 22113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281870"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFB52_12015"
     CDS             complement(<1..870)
                     /locus_tag="VFB52_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07104.1"
                     /translation="MSTDEVLSGMGSGSDPEQAGPKFTLELNQDQKDIRDWVHGFAEG
                     VVRPAAAEWDEREETPWPVIQEAAKIGLYSFEAMGQFWADETGLTLPIANEELFWGDA
                     GIGMAIMGTSLAVAAIFGQGTPEQMGEWIPQCFGTPDDVKLGAFCASEPDAGSDVSAI
                     RTSAKYDEAKDEWVLNGQKAWATNGGIANVHVVIASVDRELGSRGHAAFVIPPGTPGC
                     EQGAKVKKHGLRASHTADVHLDDCRVPGSCLLGGKDKLDERLARVREGKRAKSQAAMQ
                     TFEASRPTVGAQAL"
     gene            complement(950..1324)
                     /locus_tag="VFB52_12020"
     CDS             complement(950..1324)
                     /locus_tag="VFB52_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07105.1"
                     /translation="MTATRTELGECRQCRSFCDKMVEPRGCIELGCRFLYSYVDRLTG
                     EQYVGCMQEVFGAEIDLRAVLEPGGFGGVKMTGDPLPHCQFSVERAYEGSGDSYDCVN
                     RRFFDCTDGGSEGLRAFDLRDV"
     gene            complement(1374..4904)
                     /gene="dnaE"
                     /locus_tag="VFB52_12025"
     CDS             complement(1374..4904)
                     /gene="dnaE"
                     /locus_tag="VFB52_12025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZO07106.1"
                     /translation="MSTPAVHLHAHSEYSLLDGACKIDAMAARAAELDQPALGLTDHG
                     VMNGAVDLYKACKKHGIKPVVGLEAYLVDDRVAIKEQTRYERNHLTLLAENDTGFANL
                     VKLTSAGFLEGFSRGKANVDMELLATHAEGVIVLTGCLQSRFCRRLLEERADDARAHL
                     DDLIQAFGAEQVYFEVQKNGIDDQDKANEGIVRFARELGRPLVATADVHYLRREDFDN
                     HAALLCVQTKSTLEAPKMSFDTNEFYIKSNEEMHDSFAEWPEAVPTTLEIAERVDLDI
                     ELGSLLLPRYPTETGEEPEQMLRRIAGEGLRARYGDPPPAEAVERLEYEIGVIEEMGF
                     SSYFLIVWDFIRYAKENGVAVGPGRGSAAGSIVSYILNITDLDPLANDLLFERFLNPG
                     RKSMPDIDIDFSVRGRERMIRYVGEKYGRESVAQIITFGKMAPRAATRDAARVLGYDY
                     GTGDRLAKQIPEPIMGRSPSFEECLRNGQELKQTYDAEPDAKKIIDVAQGLEGIIRNN
                     SIHAAAVVIADRPLQEIVPLQLAEDRSAPAAAPNGSGSGKPERQYKIVTQYSMGPIEE
                     MGLLKMDFLGLRNLDVIEDAIDIIERSRGERIEVEKIPIDDAKTFEMLARGDSTGVFQ
                     LESEGMREAMKKVRPTEFDDIVALVALYRPGAMAYIPAYAKGKRDPSSVTYPDQRLRA
                     FTESTYGCVLYQEQLMAIAREMAGFSGGEADDLRKAVGKKKRDLMATMKDKFMQGLAE
                     SGTAPNVAADLWKLNEAAADYSFNKSHAACYGLISYRTAYLKANYPAEYMAAVISSVM
                     NTKDKVPFFVNRCAEMGIDVLPPDVNSSDHSFVVSENAIRFGLDAVKNVGHQAVEAIL
                     RAREDKEIASIWDFCERVDSRAVNKRAIECLIKCGALDSTGATRKGMLEALPAAQSAG
                     QKAQEDAQMGQGSIFDFGGEAGGEAAQSQAVHQRPPISAVEFDRAELLALEKETLGTY
                     LSSHPLAEVGPALRQRVDCSLAQLAGKPDGAWVTVGGIVVDCKKIRTKSGNQMMFATL
                     DDVEGQVEMLVFKADQAESAAVIQPDAIVLVRGRLDHKDRGETKLVVQEASRFEPDSA
                     EIASAAKAMPAVSAGPFELTLALADLSPELLAELKAVCEHHKGDSDVHVVVKTSEGDK
                     RLRFGSDYRVRPSAGLKNDLDQILASPLAA"
     gene            5511..5918
                     /locus_tag="VFB52_12030"
     CDS             5511..5918
                     /locus_tag="VFB52_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZO07107.1"
                     /translation="MNYMLLLMGEEPNWEELSPEEAQANLDEMGAYNAELMEAGAMVT
                     GGGLESRSTATVVRFDTGGETTVTDGPFIESKEQLMGFWIIDCANLDEALAWAKKVPL
                     RGASLEVRPLIGTAGEEVDGEELIRRGSGQRSE"
     gene            5931..7178
                     /locus_tag="VFB52_12035"
     CDS             5931..7178
                     /locus_tag="VFB52_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZO07108.1"
                     /translation="MAPGDRDSVVDRTFRQESGRAVATLIRVLRDFDAAEEAVQEAFV
                     AALERWPRHGVPANPGAWITQVARNKAIDRVRRERNLRDKEAVLERLEALRPPIAGPA
                     EALAAQGEIEDDRLRLVFTACHPALAPEARVALTLRTLGGLSTAEIARAFLIAEPTMA
                     QRLVRAKGKIAKAGIPYRVPDAEQMPERLPSVLVTLYLVFNEGYLASSAEGLVREELC
                     AEAIRLARVTHSMLPAEPELTGLLALMLLNDARRPARIDAQGEAVPLERQDRTLWDKE
                     KIAEGLALSEQAAAAGPVGPYTVQARIAAAHAVTVRPEETDWARIVRLYEWLAVVAPS
                     PVVDLNRAVAVAMNEGPERGLELIDSIEGLDDYQPLHASRADLLRRLGRTGEAAAAYA
                     RALELSANPAERRHIEDRIAEAS"
     gene            complement(7186..7398)
                     /locus_tag="VFB52_12040"
     CDS             complement(7186..7398)
                     /locus_tag="VFB52_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07109.1"
                     /translation="MEAMRKAWTDDRLDDLRDEMRSGFARVDADIREIRGELGSLHRT
                     MVVGFATIGGGVLASVVATVLAAVLG"
     gene            complement(7484..8500)
                     /locus_tag="VFB52_12045"
     CDS             complement(7484..8500)
                     /locus_tag="VFB52_12045"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZO07110.1"
                     /translation="MRAQLRRDFLAIGVATALFAALVAVVLAQAPAAGAADAEQLEGK
                     LASARDEAGALASELRQSREALAGAESEAAAAEARESELSGLLAEGQERTAALSRRVE
                     STREELARAKARLRRARAALAARLVAIYESGSPDTASVVLGAGDYDELVTRSLYLSLI
                     AESDDALAERVGEVRDEVAGDVEAVEALEQEAVEHEEELAAARDEIAGARAAAQASAA
                     ELQSLSASREASLSNLRGKIDGWVEEVAEAQASKAEAEAEVGRWLGGPYSIPTYIVMC
                     ESGGDYSALNPSSGAGGAYQIIPSTWDLYGGKGEPQNAPKAEQDRIAAEIWADSGGSA
                     WVCA"
     gene            complement(8582..8734)
                     /locus_tag="VFB52_12050"
     CDS             complement(8582..8734)
                     /locus_tag="VFB52_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07111.1"
                     /translation="MYDKVGKTAVKFAYRYLRWRYKREIRIGLGLAAAGIAVAGLIAS
                     RNVREG"
     gene            complement(8734..10014)
                     /locus_tag="VFB52_12055"
     CDS             complement(8734..10014)
                     /locus_tag="VFB52_12055"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZO07112.1"
                     /translation="MSAEPQHPQPTASYEADPAQVDRVLLLYSGGLDTSVMLKWIQDS
                     YEAEVVCLTVNLGQPGEDYSVIEDKALALGAVECHVVDAREQFAAQFIAPAIKANAVY
                     GNGYPLFTALGRPLIAQLAVEYARKTNCDTIAHGCTGKGNDQVRIEATVATLAPELKT
                     IAPVRSWQMGRDEEIAYAREHGIPVKGGTEAAPYSIDDNLWGRSSEGKWIEDLDHAPE
                     DDVFQLVTRPELAPDEAETVTVGFERGLPVLLNGERLGLVDLLEKAAEIGCRHGVGIL
                     DHIEDRIVGLKVRDIYEAPAAAILLTAHEELERLVGTIHQNQFKPALDQKWGYLVYAG
                     LWWEPLRSDLDAYMDAVNAQVTGEIGLRLYKGSVRVVTRSSPNAVYDAQLAGFAESGG
                     LFSQTASPGFIELWSLQSRLAHQIRQEGGGEEES"
     gene            complement(10033..10431)
                     /locus_tag="VFB52_12060"
     CDS             complement(10033..10431)
                     /locus_tag="VFB52_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07113.1"
                     /translation="MHEDPTRMMRQPEDDPHGRGGGDGGRPHGNMRLLVACLIAVIVG
                     LVIAVIVIAGDDGGGSGTETVSTSESTTTETTTTPDTTEKPTTEDTTAEEPEEPEEEL
                     EEEEPAPEEEGGEGGISPEEEGGGGGIEAP"
     gene            complement(10435..11010)
                     /locus_tag="VFB52_12065"
     CDS             complement(10435..11010)
                     /locus_tag="VFB52_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07114.1"
                     /translation="MRRSKDRKDEPQGRPPAGGSDVVSEASERLATIVNAAERAAEQV
                     IDDAEARAREILAEAEADADKIRGEAIAAVERARRELGALTSEPESGPEQVSAPPSRT
                     NHLSAVPTTDEPEPEGSAQLAAATPPPDAEPEPTPAGGSKRGTPAGARLLATQMAVSG
                     SSREEIEARLRNGFEIEDTTAILDAILGPED"
     gene            complement(11022..12218)
                     /locus_tag="VFB52_12070"
     CDS             complement(11022..12218)
                     /locus_tag="VFB52_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZO07115.1"
                     /translation="MSTETGTSLGLEETRALEERYLMRTYKRGAVDFVRGEGALLWDA
                     EGNEYLDFLTGISVCSLGHCHPDVVAAVREQTERLMHVSNLFYTEPMARLAQRLAESS
                     LGGRVFFANSGTEANECAIKIARKHARQRGITAPEIVSFEGEFHGRSYGSLSATPGLA
                     KNENFWPMLPGFRSVPRNDAAALREAVGENTAAVLIEPIQGEAGVYPTSEEALLAARE
                     ACDAAGALLIFDEIQTGVGRTGSLWAYQQSPVRPDVITSAKALGGGVPIGACITAPVA
                     ADVLEPGDHGSTFAGGPVATAAALAVLDVVDDPGLLRGVRERGTQLRAGLEALDGVRE
                     TRGRGLMVGVGLAEGIDAAAVGAELLRRGLVVNVPAPDTLRLLPPLVVDSDQIERAVR
                     LIGESL"
     gene            complement(12215..13084)
                     /gene="argB"
                     /locus_tag="VFB52_12075"
     CDS             complement(12215..13084)
                     /gene="argB"
                     /locus_tag="VFB52_12075"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZO07116.1"
                     /translation="MTEQAPQSRAAATSIETLLEALPYIREFHGRTVVIKYGGAAMID
                     ETLRDEFATDVVLLKYVGLNPVIVHGGGPEISRYMDKLGLEVKFHEGLRVSDAETVEV
                     AKMVLVGKLNSEIVQRLNRRGQPAVGLAGEDGSLFEVAPVANADQVGFVGSVQRVDVD
                     VLNHIAADYIPVIASSAADRDGQPYNINADEAAGKVAAALGAYKSIFLTDVEGWRAKA
                     DDENSLISRATVGEVEEALDGVDGGMRPKLQACVDAIRGGAQSAHIIDGRVPHSLLLE
                     LFTDAGIGTMVTP"
     gene            complement(13122..14306)
                     /gene="argJ"
                     /locus_tag="VFB52_12080"
     CDS             complement(13122..14306)
                     /gene="argJ"
                     /locus_tag="VFB52_12080"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZO07117.1"
                     /translation="MSVLGAPEVHGEAFFRSRWVEHPEGVEELDPAQLPPGFRAGGAH
                     CGLKKGGETDIGVIVCDEPEVRSAMVLTRNASAAAPIRVCRKVTELDGVRAAVVNSGN
                     ANAETGEQGFADALGMCERTAAALGLRSEQIAVAETGTIGVPLPISAVLPGIDEAASA
                     LSPRGGIDFSAAICTTDRWAKCCTLKVGGVTISAQAKGAGMIEPNMATMLCFVQTDAV
                     VDDPDKLLREATDASFNRITVDGQMSTNDCVLLQATGTSGEPLPAGLLEAVLLQLAIE
                     IVRDGEGAVRVGRVEVSEAASAAEAERVARAIANSPLVKTALYGRDPNWGRIAQAAGA
                     ALAGEDLEEIGADSIDAAELGGEADEAEIGLRLGRGEHRAHVWFSDLGYEYIRINAEY
                     TT"
     gene            complement(14303..15337)
                     /gene="argC"
                     /locus_tag="VFB52_12085"
     CDS             complement(14303..15337)
                     /gene="argC"
                     /locus_tag="VFB52_12085"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZO07118.1"
                     /translation="MNELNSQVLDNPVARVLVAGANGFTGALAAKIVWEHPRLELVAA
                     TSRSDAGKRIDEIYPRYRVPVTLTELDLDTLSNLGADAAIVAYPHGAAAPTVAALREA
                     GVLVVDLSADFRLRDMATYERWYGEHGAPGLFGTGVYGLSEMYREELREATLVATPGC
                     YPTASVLGLAPLAEKGLVTDVTIAAMQGTSGYGRSSDDLVHFSSMTENAFPYKTEGHR
                     HRPEIEQELEALGSQATVAFVPHLLPLDQGELATCFATLAEPTSKDAVVALYRERYAD
                     EPFVHLLDGPPNLRALRDTNECHVYVTVEEHGKVMAFSAIDNIWKGASSQGVQNLNLM
                     LGIEETEGLT"
     gene            complement(15330..17792)
                     /gene="pheT"
                     /locus_tag="VFB52_12090"
     CDS             complement(15330..17792)
                     /gene="pheT"
                     /locus_tag="VFB52_12090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZO07119.1"
                     /translation="MRVPLSWLREYCDPGVDPEQLADRLVMTGTEVERLAVVGPPSAE
                     NFIVGKVLSAEQHPDADRLRVCTVDDGSGEERTIVCGAPNVGAGQTVAVALPGARMPG
                     GEKLRKAKLRGVASEGMILAADELEVGEDHDGIMVLDDALVAGTPLSEVLPLAEPVLE
                     IEVTPNRVDCFGVHGVAREVHAISGAPLAADPWAEDAAAEGEGAVEDYASVAVDVPEL
                     CPRFTARVFTDVTVGPSPLWLQARLTAAGQRPINNVVDITNYVMLLTAQPLHAFDLDK
                     VPDGELSVRTAAAGERMTTLDGVERELDAQTVLVSDRDGATGVAGIMGGQVSEVSETT
                     TRVLLEVANWNGTNILRTSRLLGLRSEASSRFEKQLHPDLCLRAQAVASKLMVELCGA
                     KLVPGTIDVAAEIPAPRELTLRAARVGGLLGMEITQAEQTEYLERLGFKVAPVGEDLR
                     VTVPPDRHYDVTREVDLIEEVARVHGIDEHLPTTLPTAAGRVGGLSREQRLRRRAEDG
                     LRDLGFDQVVGWSFTDPGEPVRLRIPAGDPRANPALVSNPLSEDQSLMRTTVLGSLLD
                     VAARNAARGADRLALFEAGAVYLQGDPPGEGSALAGNFRGERAAPVTEPHRYAVLALG
                     PLVERSWRGGGEPADFFALKGVLEGLAAQLGATLEFAPGEQPFLHPGRSATVSIGGTP
                     AGWLGEVHPLVCREWDLDAAVAFEIDAAPLLAAATAGEESFEDIAEFPPVLQDISVVV
                     SEGVPFAEVEAAVLGAGGELLRSTRLIDSYAGEQIGAGKVSLTLTLEFRAADRTLTDD
                     EVAGQREAIKAGLEAIGGALRE"
     gene            complement(17803..18861)
                     /gene="pheS"
                     /locus_tag="VFB52_12095"
     CDS             complement(17803..18861)
                     /gene="pheS"
                     /locus_tag="VFB52_12095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZO07120.1"
                     /translation="MLDRIEGIRAAGAEAIAAADSSAALEELRVSYLGRKAELTTILR
                     GIAELPQEERGQVGGAANKARKQLEALIDEASARLDASELDAKLLADRVDVTLPGAPP
                     RPVGHAHLIARTTRQIEDIMVGLGYTVAEGPEIEHDYYNFTALNHPEGHPARMLQDTF
                     YVQSHPEVLLRTHTSPMQIRAMETQQPPIFVIVPGKVYRRDSDATHSPMFHQIEGLAI
                     AEGITLADLQGTLLALCQAIFGDEREVRLRPHFFPFTEPSVEVDVSCFQCGGSGVLEG
                     GERCNLCKGQGWIEILGAGMVDPNVLGFVEQHGYDPEKFQGFAFGLGVERIAMLRHGV
                     PDLRRFFDNDVRMLEQFS"
     gene            complement(18854..19570)
                     /locus_tag="VFB52_12100"
     CDS             complement(18854..19570)
                     /locus_tag="VFB52_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZO07121.1"
                     /translation="MIESPQNEKLKLVRKLRERKHREREGLFATEGEDLLEAGLAAGA
                     VPRLVLTAPDAEIDGEEVDPDLLAGVSTLGSGTRVIAIWPLTWAERAEAPCLYLHGVA
                     DPGNVGTIIRTADALIGSSVVLGPECADPFSPKAVRASMGSVFSQPPLRGGVEETPEP
                     RTALVAHGGEDLGALAGTATICLGAEREGLPGEVVSRCGTQVTIPLRGGAESLNVAAA
                     AAIAAQRISSPAMNGGQPDA"
     gene            complement(19679..20038)
                     /gene="rplT"
                     /locus_tag="VFB52_12105"
     CDS             complement(19679..20038)
                     /gene="rplT"
                     /locus_tag="VFB52_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZO07122.1"
                     /translation="MARVKRSVHARKKRRKVLKEAKGYFGRKHSSYRFAKEQVQRSGM
                     YAYRDRRNKKRTFRRLWITRINAAARLEGMSYSELMHGLNKAEVEVNRKMLADIAVHD
                     REAFRRFVELAREGAAA"
     gene            complement(20039..20236)
                     /gene="rpmI"
                     /locus_tag="VFB52_12110"
     CDS             complement(20039..20236)
                     /gene="rpmI"
                     /locus_tag="VFB52_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZO07123.1"
                     /translation="MPKMKTHSGAKKRFKKTATGKLRGRRAYSSHILEKKSPKRKRKM
                     AKPVDISPNDAKTVRTLLGGK"
     gene            20248..21405
                     /gene="wecB"
                     /locus_tag="VFB52_12115"
     CDS             20248..21405
                     /gene="wecB"
                     /locus_tag="VFB52_12115"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZO07124.1"
                     /translation="MKQTRAASGLAGFSLHLVAPTHAGTLRAMRIVYVVGTRPNFVKT
                     APVIGALRERLPEGRHAIVHTGQHYDRLMSEVFLEELGVPAPDHMLEVGSASHAVQTA
                     RTMERLEPVLLEERPDLVIVPGDVNSTLAATLTAVKLQIPLAHVEAGLRSFDMAMPEE
                     ANRIVADRFAEFLFLHSEEATGNLRAEGIPEERMHFVGNTMIDTLVALEERIRAADAA
                     AAQGLTPGEYVLVTLHRPALVDGPLLGETVAQLAALAEQMPVVFPVHPRTRKMMEGAG
                     AADSPGLRLIEPLGYLDFLSLMADAGAVLTDSGGIQEETTYLGIPCFTLRDNTERPIT
                     ISAGTNTLLGLDPAAIAGIPSALAARGNPPTKPPPLWDGNAATRIADVIAS"
     gene            complement(21406..22065)
                     /gene="infC"
                     /locus_tag="VFB52_12120"
     CDS             complement(21406..22065)
                     /gene="infC"
                     /locus_tag="VFB52_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZO07125.1"
                     /translation="MPVPRRFDRRPPERDTTRINERIRVPQVRLIGADGEQLGIVDTA
                     EALRQAQAADLDLVEVAPNSKPPVTRLLDYSKYKYEQEQKQKQARKHQQQVNIREIKL
                     RPKIATHDYETKKGHVERFLKHHDKVKVTIMFRGREQAHPERGRDLLQKLFEDLGGIA
                     VIESAPLQEGRNMSMLLAPSKEVDKIAGTDSTDAPAAEAPAADAPAAEAPAADSPPAD
                     A"
BASE COUNT         3088 a         7774 c         7656 g         3595 t
ORIGIN      
        1 gagcgcctgt gcaccgaccg tcggccggct cgcctcaaaa gtctgcatcg ccgcctgtga
       61 cttcgcccgt ttgccctcgc ggacccgcgc caggcgttcg tcgagcttgt ctttgccgcc
      121 cagcaggcag gagcctggca cgcggcagtc gtccagatgg acgtcggcgg tatgcgaggc
      181 gcgcaggccg tgcttcttca ccttggcgcc ctgctcgcag cccggcgtgc cgggcgggat
      241 cacgaaggcc gcgtggccgc gcgagccgag ctcgcggtcg accgaggcga tcaccacgtg
      301 cacgttggcg atgccgccgt tggtcgccca ggccttctgg ccgttcagca cccactcgtc
      361 cttggcttcg tcgtatttgg cgctcgtgcg aatcgccgag acgtccgagc cggcgtcagg
      421 ctccgaggcg cagaaggccc cgagcttgac gtcgtccgga gtgccaaagc actgggggat
      481 ccactccccc atctgctccg gggttccctg cccgaagatt gccgcgaccg cgagcgaggt
      541 gcccatgatc gccatgccga tgccggcgtc gccccagaac agctcctcgt tggcgatcgg
      601 cagggtgagg ccggtctcgt cggcccagaa ctgccccatc gcttcgaagc tgtagaggcc
      661 gatcttggcg gcctcctgga tcaccggcca gggcgtctcc tcgcgctcgt cccattcggc
      721 ggcagcgggg cggacgacgc cctcggcaaa gccgtggacc cagtcgcgga tgtccttctg
      781 atcctgattg agctcgaggg tgaacttggg accggcctgc tcggggtcag aaccgcttcc
      841 catcccggac agaacctcgt cagtgctcac ctcggcgaca gcagacgcct ctggcatggc
      901 ggcgatatta cacgaattcg tgtcgtctct acacgaatca gtgtcgccgt cagacgtctc
      961 tcagatcgaa ggcgcgcagg ccctcggagc ccccgtcggt gcagtcgaag aagcgccggt
     1021 tgacgcagtc gtaggagtcg cccgagccct cgtaggcgcg ctcgaccgag aactggcagt
     1081 gaggcagcgg atcaccggtc atcttcaccc cgccgaaccc gccgggctcg agcaccgccc
     1141 tcaggtcgat ctcggcgccg aacacttcct gcatgcagcc gacgtactgc tcaccggtca
     1201 gccggtcgac gtagctgtag agaaaccggc aaccgagctc gatgcaaccg cgcggctcga
     1261 ccatcttgtc gcagaaggag cggcactggc ggcactcgcc gagctcggtg cgcgtagcgg
     1321 tcataacgaa agtctaggga gccgagaccg cggaaccaag cggtttgtgg ctttcaagcg
     1381 gccagcgggc tggcgaggat ctgatcgaga tcgttcttca ggccggcgga gggccggacc
     1441 cggtagtcgc tgccgaagcg cagccgcttg tcgccctcgc tggtcttgac gacgacgtgg
     1501 acgtcggagt cgcctttgtg gtgttcgcag accgccttca gctcggccag cagctcgggg
     1561 ctgaggtcgg cgagcgcgag ggtgagctcg aacgggcccg cgctcaccgc cggcatcgcc
     1621 ttcgcggcgc tggcgatctc cgcgctgtcg ggttcgaagc gactcgcctc ctgcacgacc
     1681 agcttggtct cgccgcgatc cttgtggtcg aggcggccac gcacgagcac gatcgcgtcc
     1741 ggctggatca ccgcggcgct ctcggcctgg tcggccttga agaccagcat ctcgacctgg
     1801 ccctcgacgt cgtcgagggt cgcgaacatc atctggttgc cgctcttggt gcggattttt
     1861 ttgcagtcga cgacgatccc gccgaccgtc acccaggcgc cgtcgggctt gccggcgagc
     1921 tgcgcgaggc tgcagtcgac ccgctggcgc agcgccgggc cgacttcggc cagcggatgc
     1981 gaggagaggt aggtgccgag cgtctccttc tccagggcca gcagctcggc gcggtcgaac
     2041 tcgaccgccg agatcggcgg ccgctggtgg acggcctgcg actgcgccgc ctctcccccg
     2101 gcctcgccgc cgaagtcgaa gatcgagccc tggcccatct gcgcgtcctc ctgggccttc
     2161 tgcccggccg actgcgccgc cggcagcgct tccagcatcc ctttgcgggt cgccccggtc
     2221 gagtcgagag cgccgcactt gatcaggcat tcgatcgcgc gtttgttgac cgcgcgcgag
     2281 tcgacccgct cgcagaagtc ccagatcgac gcgatctctt tgtcctcgcg ggcgcgcagg
     2341 atcgcctcga ccgcctggtg gccgacgttc ttgaccgcgt cgaggccgaa gcgaatcgcg
     2401 ttctcggaga cgacgaagct gtggtcggag gagttgacgt cgggcggcag gacgtcgatc
     2461 cccatctccg cgcagcggtt gacgaagaac ggcaccttgt ctttggtgtt catcaccgag
     2521 gagatcaccg ccgccatgta ctcggccggg tagttggcct tgaggtaggc ggtgcggtag
     2581 gagatcagcc cgtagcaagc ggcgtgggac ttgttgaacg agtagtcggc ggccgcttcg
     2641 ttcagcttcc agaggtcggc ggcgacgttg ggcgcggtcc ccgattcggc caggccctgc
     2701 atgaatttgt ctttcatcgt cgccatcagg tcgcgcttct tcttgccgac cgccttgcgc
     2761 aggtcgtccg cctcaccgcc ggagaagccg gccatctcgc gggcgatcgc catcaactgc
     2821 tcctggtaaa ggacgcagcc gtaggtcgac tcggtgaagg cgcgcaagcg ctggtcgggg
     2881 taggtgaccg aggagggatc gcgcttgccc ttcgcgtagg cggggatgta ggccatcgcg
     2941 ccggggcggt agagggcgac gagcgcgacg atgtcgtcga actcggtcgg ccgcaccttc
     3001 ttcatcgcct cgcgcatgcc ctcggactcg agctggaaga cgccggtcga gtcgcccctg
     3061 gcgagcatct cgaaggtttt cgcgtcgtcg atcgggatct tctcgacctc gatccgctcg
     3121 ccgcgcgagc gctcgatgat gtcgatcgcg tcctcgatca cgtcgaggtt gcggaggccg
     3181 aggaagtcca tcttcagcag ccccatctcc tcgatcggcc ccatcgagta ctgggtcacg
     3241 atcttgtact ggcgctccgg cttgcccgaa ccgctcccgt tcggggcggc ggcgggagcg
     3301 ctgcggtcct cggccagctg cagcgggacg atctcctgca gcggccggtc ggcgatcacc
     3361 accgcggcgg cgtggatcga gttgttgcgg atgatcccct cgagcccctg cgcaacgtcg
     3421 atgatttttt tcgcgtcggg ctcggcgtcg taggtctgtt tcagctcttg cccgtttctc
     3481 aggcactcct cgaaggacgg gctgcgaccc atgatcggct ccgggatctg cttcgccagc
     3541 cggtcgccgg tcccgtagtc gtagccgagc acgcgggcgg cgtcgcgggt cgcggcgcgc
     3601 ggggccatct tgccgaaggt gatgatctgg gcgaccgatt cgcggccgta tttctcgccg
     3661 acgtagcgaa tcatccgctc gcgcccgcgg accgagaaat cgatgtcgat atccggcatc
     3721 gatttacggc cggggttgag gaagcgctcg aacaggaggt cgttggcgag cgggtcgagg
     3781 tcggtgatgt tgaggatgta ggagacgatc gagccggccg ccgagccacg gccgggaccg
     3841 acggcgacgc cgttttcctt cgcgtagcgg atgaagtccc agacgatcag gaagtaggag
     3901 ctgaagccca tctcctcgat cacgccgatc tcgtactcga gccgctcgac cgcctcggcc
     3961 ggcggcgggt cgccgtagcg ggcgcgcagt ccctcgccgg cgatccggcg cagcatctgc
     4021 tcgggctcct caccggtttc ggtcgggtag cggggcagca gcaggctgcc cagctcgatg
     4081 tcgaggtcga cccgttcggc gatctccagc gtggtcggca ccgcctccgg ccactcggcg
     4141 aacgagtcat gcatttcttc gttgctcttg atgtagaact cgttcgtgtc gaagctcatc
     4201 ttcggcgctt ccagggtcga cttcgtctgc acgcagagca gtgcagcgtg gttgtcgaag
     4261 tcctcgcgcc gcaggtagtg gacgtcggcg gtggcgacca gcgggcggcc gagctcgcgg
     4321 gcgaagcgca cgatcccctc gttggccttg tcctggtcgt cgatcccgtt cttctgcacc
     4381 tcgaaataga cctgctcggc gccaaaggcc tggatcaggt cgtcgaggtg ggcgcgcgcg
     4441 tcgtcggccc gctcttcgag caggcgccgg cagaagcgcg actgcaggca gccggtgagc
     4501 acgatcacgc cctccgcgtg ggtggcgagc agctccatgt cgacgttggc cttgccgcgc
     4561 gagaagcctt cgaggaagcc cgccgaggtg agcttgacca ggttggcgaa gccggtgtcg
     4621 ttttcggcca gcagggtcag gtggttgcgc tcgtagcgcg tctgctcctt gatcgcgacc
     4681 cggtcgtcga ccaggtaggc ctcgagcccg acgaccggtt tgatcccgtg cttcttgcag
     4741 gccttgtaga ggtcgaccgc cccgttcatc accccgtgat cggtcagccc cagcgccggc
     4801 tgatcgagct ccgcagcccg cgccgccatc gcgtcgatct tgcaagctcc gtccagcagc
     4861 gagtactcgc tgtgggcatg gagatgaacg gcgggcgtgc tcacggacga gcgatcatgg
     4921 caatcgaacc cgacggaccc gaccctcttt cacgcaaatc aggacctaat ttcaaacaag
     4981 atcgggacgg gccgcagcgg ggtcggggga ggggttccac ttagtctttt cgacatcgag
     5041 ccccggccat gcggcgatgg aatgcgccga aaagccacaa gacgttccac ccctcccccg
     5101 accccgcttc gaatcgcccc agcttgtgcg agcttgggtt gctctagcgc tcacgggagc
     5161 cgcgtgcttc agggcgactc cctcgttcgc gcctccagaa gggcctcgtc atccccacgc
     5221 cgccgagcgt gggtgcagct gcacgtcgct ctagagctgt tggcatggct tcgaagcgtg
     5281 agcaagggga cccggtaggg gtgaaacgtc ttgtgttttt tccgcggagc tcctccgccg
     5341 caaggccggt gctcgatgtc gaaaaaacta agtggaaccc ctaccgggtc cccttgctcc
     5401 ccctccaagc caattcgaaa gttttaggcg acctgtcgat ctggccactc ctcgttcgtc
     5461 gttagggtga aggcggccat tcgggctgcc gtgatcaagg aggaaccgcg atgaactaca
     5521 tgctgctgct gatgggcgag gaacccaatt gggaggagct ttcgccggag gaagcgcagg
     5581 ccaacctcga tgagatgggt gcctacaacg cggagctgat ggaggctggg gcgatggtca
     5641 ccggcggcgg attggagagc cgcagcacgg caaccgtcgt ccgcttcgac accggcggtg
     5701 agaccacggt caccgacggc cccttcatcg agagcaagga gcagctgatg ggcttctgga
     5761 tcatcgactg cgccaacctc gacgaggccc tcgcctgggc gaagaaagtg ccgctgcggg
     5821 gcgcctcgct cgaagtgcgg ccgctgatcg gcaccgccgg cgaagaggtc gacggcgagg
     5881 agctgatccg gcgcggctcg ggccagcgct ccgagtagct aaccgggccc ttggcccccg
     5941 gggaccgcga cagcgtcgtc gatcgcacct tccggcagga gtcgggaaga gcggtggcga
     6001 cgctgatccg cgttctgcgt gacttcgacg ccgccgaaga ggcggtccag gaagcattcg
     6061 tggcggcgct ggagcgctgg ccgcgccacg gcgtccccgc caaccccggc gcctggatca
     6121 cccaggtcgc ccgcaacaag gcgatcgacc gggtgcgccg cgagcgcaac ctgcgcgaca
     6181 aggaggcggt gctggaacgg ctggaggcgc tgcgaccgcc gatcgccggc ccggccgagg
     6241 cgctcgccgc ccagggcgag atcgaggacg accggctgcg cctggtcttc accgcctgcc
     6301 acccggcgct ggccccggag gcgcgggtgg cactgaccct gcgaacgctt ggcggccttt
     6361 caaccgcgga gatcgcccgc gccttcctga tcgcggagcc gacgatggcg cagcggctgg
     6421 tccgggcaaa aggaaagatc gccaaggccg ggatccctta tcgggtgccc gacgccgagc
     6481 agatgcccga gcgcctgcca agcgtccttg tgaccctcta cctggtcttc aacgagggct
     6541 acctcgcctc cagcgccgag gggctggtgc gcgaggaact gtgcgccgag gcaatccgac
     6601 tggcccgcgt cacccactcg atgctgccgg cggagccgga gctgaccggc ctgctggcgc
     6661 tgatgctgct gaacgacgcg cgacgcccgg cgcgaatcga cgcgcagggc gaagcggtgc
     6721 cactcgaacg ccaggaccgc acgctctggg acaaggagaa gatcgccgag ggtctcgccc
     6781 tctccgaaca ggcggccgcc gccggcccgg tcgggcccta caccgtgcag gcgcggatcg
     6841 cggccgccca cgcggtcacc gtgcgaccgg aggagaccga ctgggcgcga atcgtgcgcc
     6901 tctacgaatg gctcgcggtg gtcgcgccga gtccggtggt cgacctgaac cgggcggtgg
     6961 cggtggcgat gaacgagggc cccgagcgcg ggctcgagct gatcgactcg atcgagggcc
     7021 tcgacgacta ccagcccctg cacgcttccc gcgccgacct cctgcgtcgc ctcgggcgga
     7081 ccggcgaggc cgcagccgcc tacgcccgcg cgctggagct gagcgcgaac ccggccgagc
     7141 gccgccacat cgaagaccga atcgccgaag cgagttgaac gagcctcaac cgagtaccgc
     7201 ggcgagaacc gtcgcaacca cagaagcgag cacaccaccg ccaatcgtcg cgaaaccgac
     7261 gaccatcgtc cggtgcaggg atccgagctc gccgcgaatt tcgcgaatgt cggcatcgac
     7321 gcgagcgaaa ccactgcgca tctcgtctcg caggtcgtcc agccgatcgt cggtccaggc
     7381 tttcctcatc gcctccatga tggcaagcct acgaggcgtt ttgtctgctg tggcgcgcag
     7441 ttcgcagaat cttcatggcg ccgatgtggc gaaagggtga cggctaggcg cagacccagg
     7501 cggagccgcc tgagtcggcc cagatttcgg cggcgatccg gtcctgttcg gccttcgggg
     7561 cgttctgggg ttcgcccttg ccgccgtaga ggtcccaggt cgaggggatg atctggtacg
     7621 cgccgccggc gccgctggag gggttgaggg cgctgtagtc gccgcccgac tcgcacatca
     7681 cgatgtaggt ggggatcgag taggggccgc cgagccagcg gccgacctcg gcttcggctt
     7741 ccgccttgct ggcctgcgct tcggccacct cttcgaccca gccgtcgatc ttgccgcgca
     7801 ggttcgagag cgaggcctcg cggctggcgc tcagcgactg cagctcggcc gcactcgcct
     7861 gcgccgccgc gcgggcgccg gcgatttcgt cacgggcggc ggccagctct tcttcgtgtt
     7921 cgaccgcttc ctgctccagc gcttcgaccg cctcgacgtc gccggcgact tcgtcgcgga
     7981 cctcgccgac ccgctcggcg agggcgtcgt cggattcggc gatcaggctc agatagagac
     8041 tgcgggtgac aagttcgtcg tagtcgccgg cgccgaggac gacgctggcg gtgtccggcg
     8101 agccgctttc gtagatcgcg accaggcggg cggcaagcgc ggcgcgggcc cgccgaaggc
     8161 gagctttggc ccgtgccagt tcctcccggg tgctctcgac ccggcggctg agcgccgcgg
     8221 tccgctcctg gccttcggcc agcagccccg agagttcgct ctcgcgggct tccgccgcgg
     8281 ccgcttcgct ttcggctccg gccagcgctt cgcggctctg gcgcagttcg ctcgccaggg
     8341 cgccggcttc gtcgcgggcg ctcgccagct tgccttcgag ctgctcggcg tcggcggcgc
     8401 cggccgcggg cgcctgcgcc agcacgacgg ccacaagcgc cgcgaacagc gccgtcgcga
     8461 cgccgatcgc gaggaagtca cgtctgagtt gagcccgcac cacggaggca gcgaggctag
     8521 cggactttgt tagcgaccct taataagttg acgataaggc gcaacgaaag ttgcgccggc
     8581 ctcagccctc gcggacgttg cggctggcga tcaagccggc gaccgcgatc ccggccgcgg
     8641 ccagcccgag gccgatccgg atctcgcgct tgtagcgcca gcgcaggtag cggtaggcga
     8701 acttgaccgc ggttttgccg accttgtcgt acatcagctc tcttcttccc ccccgccctc
     8761 ttggcgaatc tggtgcgcca gccgcgactg cagcgaccag agctcgatga agcccggtga
     8821 ggcggtctgc gagaacaacc cgccggactc ggcgaagccg gccagctggg cgtcgtagac
     8881 cgcgttcggc gaggagcggg tgacgacgcg gacgctgcct ttgtagaggc gcaatccgat
     8941 ctcgccggtc acctgcgcgt tgaccgcgtc catgtaggcg tcgaggtcgc tgcgcagcgg
     9001 ctcccaccag aggcccgcgt agaccaggta gccccacttc tggtcgagcg ccggcttgaa
     9061 ctggttctgg tggatcgtcc cgaccaggcg ctcgagctcc tcgtgcgcgg tcagcaggat
     9121 cgccgccgcc ggcgcctcgt agatgtcgcg gaccttgagc ccgacgatcc ggtcctcgat
     9181 gtggtcgagg atcccgaccc cgtggcgaca gccgatctcg gccgcctttt ccagcaggtc
     9241 gaccagaccg aggcgctcgc cgttcagcaa gaccgggaga ccccgctcaa agccgacggt
     9301 gaccgtctcg gcctcgtccg gtgcaagctc gggccgggtg acgagctgga agacgtcgtc
     9361 ctcgggcgcg tggtcgaggt cctcgatcca cttgccctca gaggagcggc cccagaggtt
     9421 gtcgtcgatc gagtacggcg ccgcctcggt accgcccttg accgggatcc cgtgctcgcg
     9481 cgcgtaggcg atctcctcgt cgcggcccat ctgccaggag cgcaccggcg cgatcgtttt
     9541 cagctccggc gccagcgtcg ccaccgtcgc ctcgatccgg acctggtcgt tgcccttgcc
     9601 ggtgcagccg tgggcgatcg tgtcgcagtt ggtcttgcgc gcgtactcga ccgcgagctg
     9661 cgcgatcagc ggccggccga gcgccgtgaa cagcgggtag ccgttgccgt agaccgcgtt
     9721 cgccttgatc gccggggcga tgaactgcgc ggcgaactgc tcgcgcgcgt cgaccacgtg
     9781 gcactcgacg gcacccagcg ccagcgcctt gtcctcgatc accgagtagt cctcgccggg
     9841 ctggccgagg ttgaccgtga ggcagacgac ttcggcctcg taggagtcct ggatccactt
     9901 cagcatcacg ctggtgtcga ggccgcccga gtagagcagc agcacgcgat ctacctgcgc
     9961 ggggtccgcc tcgtagctgg cggtcggctg gggatgttgc ggctcggcgc tcacggggga
    10021 gcgcaagact agctagggag cttcgatgcc gcccccgccg ccttcttctt caggggagat
    10081 gccgccttcg ccgccttctt cttcgggcgc cggttcctct tcttcgagtt cttcttccgg
    10141 ttcttccggt tcttcggcgg tggtgtcttc ggtggtcggt ttctcggtcg tgtccggcgt
    10201 cgtcgtggtt tcggtcgtgg tcgattccga ggtcgagacc gtttccgtgc ccgagccgcc
    10261 gccgtcgtcg ccggcgatca cgatcacggc gatgaccaga ccgacgatca ccgcaatcag
    10321 gcaggcgacc agcaaccgca tgttgccgtg cgggcgaccg ccgtcgccgc cgccacgacc
    10381 gtgggggtcg tcttcgggct gtcgcatcat ccgggtcggg tcctcgtgca ctgctcagtc
    10441 ctccggtcca aggatcgcgt cgaggatcgc cgtcgtgtcc tcgatctcaa aaccgtttcg
    10501 cagccgcgcc tcgatctcct ctcggctgct gcccgagacg gccatctggg tggcgaggag
    10561 gcgggcgccg gcgggggtgc cgcgcttcga cccaccggcg ggcgtgggct cgggctcggc
    10621 atcgggaggc ggagtcgcgg cggcaagctg cgcagatccc tccggctccg gctcgtcggt
    10681 ggtgggcacg gcgctgaggt ggttggtccg gctcggcggt gccgacacct gctccggacc
    10741 tgactctggc tcggaggtga gcgcgccgag ctcacgccga gctcgctcga ccgcggcgat
    10801 cgcctcacca cggatcttgt cggcgtcggc ctcggcttcg gcgaggatct cgcgtgcccg
    10861 cgcttcggcg tcgtcgatca cctgctcggc ggcgcgctcg gcggcgttga cgatcgtcgc
    10921 caggcgctcg ctcgcctcgc tgaccacgtc gctgcctccg gccgggggcc gcccctgcgg
    10981 ctcgtccttg cggtctttgc tgcgcctcac tggcgcaaac cctaaagcga ttcgccgatc
    11041 aggcgaaccg cgcgctcgat ttgatccgaa tcgaccacca gcggcggcag cagacgcagg
    11101 gtgtcggggg cgggcacgtt gacgaccaga ccgcggcgca agagctccgc ccccaccgcc
    11161 gccgcgtcga tcccctcggc caacccgacc ccgaccatca ggccgcggcc gcgggtctcg
    11221 cgcacgccgt ccagcgcctc caggccggct cgcaactgtg tgccgcgctc gcgcacgccg
    11281 cgcagcagtc cggggtcgtc gaccacgtcg aggacggcga gtgcggcggc ggtcgcaacc
    11341 gggccgccgg cgaaggtcga gccgtggtcg ccgggctcga gcacgtcggc ggcaaccggc
    11401 gcggtgatgc aggcgccgat cgggacgccg ccgccgagcg ccttggcgct ggtgatcacg
    11461 tccgggcgca ccggcgactg ctggtaggcc cagagcgacc cggtcctccc caccccggtc
    11521 tggatctcgt cgaagatcag cagcgcgcca gccgcgtcgc aggcctcgcg ggcggcgagc
    11581 aacgcctcct cgctcgtcgg gtagacgccc gcctcgccct ggatcggctc gatcaggacc
    11641 gcggcggtgt tctcgccgac cgcctcgcgc agggccgcgg catcgttgcg cgggaccgag
    11701 cggaagccgg gcagcatcgg ccagaagttc tcgttcttgg ccagccccgg ggtcgccgag
    11761 agcgatccgt agctgcgccc gtgaaactcg ccctcgaagc tgacgatctc cggcgcggtg
    11821 atcccgcgct ggcgggcgtg cttgcgggcg atcttgatcg cgcactcgtt cgcctcggtg
    11881 cccgagttgg cgaaaaagac gcggccgccg aggctcgact cggccagccg ctgggccagc
    11941 cgcgccatcg gctcggtgta gaagaggttg gagacgtgca tcagccgctc ggtctgctcg
    12001 cgcaccgcgg cgaccacgtc cgggtggcag tggccgagcg agcagaccga gatcccggtg
    12061 aggaagtcga ggtactcgtt gccctcggcg tcccagagca gcgcgccctc gccgcgcacg
    12121 aagtcgaccg cgccgcgctt gtaggtgcgc atcaggtagc gctcctccag cgcccgcgtc
    12181 tcctccaggc cgaggctggt cccggtctcg gtgctcatgg ggtcaccatc gtgccgatgc
    12241 cggcgtcggt gaacagctcg agcaggaggc tgtgggggac gcggccgtcg atgatgtggg
    12301 ccgactgggc gccgccccgg atcgcgtcga cgcaggcctg cagcttcggc cgcatgccgc
    12361 cgtcgacccc gtcgagcgcc tcctcgacct cgccgaccgt cgcccgcgag atcagcgagt
    12421 tctcgtcgtc agccttggcc cgccagccct cgacgtcggt gaggaaaatc gacttgtagg
    12481 cgccgagcgc ggccgcgacc ttgccggcgg cctcgtcggc gttgatgttg tacggctggc
    12541 cgtcgcggtc ggccgccgag gaagcgatca ccgggatgta atcggcggcg atgtggttga
    12601 gcacgtcgac gtcgacccgt tgcacgctgc cgacgaagcc gacctggtcg gcgttggcga
    12661 ccggcgccac ctcgaacagc gagccgtcct cgccggccag accgaccgcg ggctgaccgc
    12721 gccggttgag ccgctggacg atttccgagt tgagcttgcc gaccaggacc atcttggcga
    12781 cctcgaccgt ctccgcgtcg gagacgcgca ggccctcgtg aaacttgacc tcgagcccga
    12841 gtttgtccat gtagcggctg atctccggcc cgccaccgtg cacgatcacc gggttgaggc
    12901 cgacgtactt gagcaggacg acgtcggtgg caaattcgtc gcgcagggtc tcgtcgatca
    12961 tcgccgcgcc gccgtacttg atcaccacgg tgcgcccgtg gaactcgcgg atgtagggca
    13021 gcgcctcgag cagcgtctcg atgctggtcg ccgccgcccg cgattgcggg gcctgctcgg
    13081 tcatcgcctg ctcctctcga tcctgtcgat gttggttgcc atcaggtcgt gtactccgcg
    13141 ttgatccgga tgtactcgta gccgaggtcg gagaaccaga cgtgggcgcg gtgctcgcca
    13201 cggccgagcc gcaacccgat ttccgcctcg tccgcctcgc cgccgagctc ggcggcgtcg
    13261 atcgagtcgg cgccgatctc ttccaggtcc tcgccggcca gcgccgcgcc ggccgcctgg
    13321 gcgatccggc cccagttcgg gtcgcgcccg taaagcgcgg tcttgaccag cggcgagttg
    13381 gcgatcgccc gcgcgacccg ctcagcctcg gccgccgagg cggcctcgct gacctcgacc
    13441 cggccgaccc ggacggcgcc ctcgccgtcg cggacgatct cgatcgccag ttgcagcagg
    13501 accgcctcga gcaggcccgc gggcagcggc tcgccgctgg tcccggtcgc ctgcagcagc
    13561 acgcagtcgt tggtgctcat ctggccgtcg acggtgatcc ggttgaagga ggcgtcggtc
    13621 gcctccctca gcagcttgtc cgggtcgtcg acgacagcgt cggtctggac gaagcagagc
    13681 atcgtcgcca tgttcggctc gatcatcccc gcccccttcg cctgggcgga gatcgtcacc
    13741 ccaccgacct tcagcgtgca gcacttggcc cagcggtcgg tcgtgcagat cgccgccgag
    13801 aagtcgatcc caccgcgcgg cgagagcgcc gacgcggcct cgtcgatccc cggcagcacg
    13861 gccgagatcg gcagcggcac gccgatcgtg ccggtctcgg cgaccgcgat ctgctcgctg
    13921 cgcagaccca gcgccgccgc ggtccgctcg cacatcccga gcgcgtcggc gaatccctgc
    13981 tcgccggtct cggcgttggc gttgccgctg ttgacgaccg ctgcccgcac cccgtcgagc
    14041 tcggtgacct tgcggcagac ccggatcggc gccgcggccg aggcgttgcg ggtcagcacc
    14101 atcgccgagc gcacctcggg ctcgtcgcag acgatcacgc cgatgtcggt ctcgccgccc
    14161 ttcttcaggc cgcagtgggc gccgccggcg cggaagccgg gcgggagctg ggcggggtcg
    14221 agctcctcga cgccctcagg gtgctccacc cagcgcgagc ggaagaaggc ctcgccgtgg
    14281 acctcggggg cgccgagcac gctcacgtca gcccctccgt ctcctcgatg ccgagcatca
    14341 ggttgaggtt ctgcacgccc tggctggagg cgcccttcca gatgttgtcg atcgccgaga
    14401 aggccatcac cttgccgtgc tcctcgaccg tgacgtagac atggcactcg ttggtgtcgc
    14461 gcagcgcccg caggttcggc ggcccgtcga gcaggtggac gaagggctcg tcggcgtagc
    14521 gctcgcggta gagcgcgacg acggcatctt tggacgtcgg ctcggcgagc gtcgcgaagc
    14581 aggtcgccag ctcgccctgg tcgagcggca ggaggtgcgg gacgaaagcg acggtcgcct
    14641 ggctgccgag cgcctccagc tcctgttcga tctccggccg gtggcggtgg ccctcggtct
    14701 tgtaggggaa ggcgttctcg gtcatcgagc tgaagtggac gaggtcgtcg ctggagcggc
    14761 cgtagccgga ggtcccctgc atcgccgcga tcgtcacgtc ggtgaccagc cccttttcgg
    14821 ccaggggggc cagcccgagc acgctcgcgg tcgggtagca gcccggcgtc gcgaccaggg
    14881 tcgcctcgcg cagctcctcc cggtacatct cgctgagccc gtagacgccg gtcccgaaca
    14941 ggcccggggc gccgtgctcg ccgtaccagc gctcgtaggt cgccatgtcg cgcaggcgga
    15001 agtcggcgga gaggtcgacg acgagcacgc cggcctcgcg cagggccgcc acggtcggcg
    15061 ccgccgcccc gtgcgggtag gcgacgatcg ccgcgtcggc gccgaggttg ctcagggtgt
    15121 cgaggtcgag ctcggtcagc gtcaccggga cccggtagcg cggatagatc tcgtcgatcc
    15181 gtttgccggc gtcgctgcgc gaggtcgccg cgaccagctc cagccgcggg tgctcccaga
    15241 cgatcttcgc cgccagcgcg ccggtgaagc cattggcgcc ggcgaccagc acccgggcga
    15301 ccgggttgtc gaggacctgt gagttcagct cattcacgca gcgcaccccc gatcgcctcc
    15361 agaccggcct tgatcgcctc gcgctgcccg gccacctcgt cgtcggtgag cgtgcggtcg
    15421 gcggcgcgga actcgagcgt cagcgtcagg ctgaccttgc ccgcgccgat ctgctcgccc
    15481 gcgtaggagt cgatcaggcg ggtcgagcgc agcagctcgc ccccggcacc cagcaccgcg
    15541 gcctcgacct ctgcgaaggg gacgccctcg gagacgacca cggagatgtc ctggaggacc
    15601 ggcgggaact cggcgatgtc ctcgaagctc tcctcgcccg cagtcgcggc ggcaagcagc
    15661 ggcgcggcgt cgatttcgaa ggcgacggcg gcgtcgaggt cccactcgcg gcagaccagc
    15721 gggtgtacct cgccgagcca gccggccggc gtgccgccga tcgagacggt cgccgagcgg
    15781 ccagggtgca ggaacggctg ctcgccgggc gcgaactcca gcgtcgcacc cagctgcgcc
    15841 gccagcccct ccagcacgcc cttgagcgcg aagaagtcgg ccggctcgcc gccgccgcgc
    15901 caggagcgct cgaccagcgg ccccagcgcc agcaccgcgt agcggtgcgg ctcggtgacc
    15961 ggcgcggcgc gctcgccgcg gaagttgcca gcgagcgcgc tgccctcgcc gggcgggtcg
    16021 ccctgcaggt agacggcgcc ggcctcgaac agcgccagcc ggtcggcgcc gcgggccgcg
    16081 ttgcgggcgg cgacgtcgag cagcgagccg agcacggtcg tccgcatcag cgactgatcc
    16141 tcggaaagcg ggtttgaaac cagcgcgggg ttggcacgcg ggtccccagc cgggatccgc
    16201 agtcgcaccg gctcgccggg gtcggtgaag ctccagccga cgacctggtc gaagccgagg
    16261 tcgcgcaggc cgtcctcggc gcggcggcgc aggcgctgct cgcggctgag gccgccgacc
    16321 cggccggcgg ccgtgggcag cgtcgtcggc aggtgctcgt cgatcccgtg cacgcgcgcg
    16381 acctcctcga tcaggtcgac ctcgcgggtg acgtcgtagt ggcggtcggg cggcacggtg
    16441 accctgaggt cctcgccgac gggcgcgacc ttgaagccga ggcgctccag gtactcggtc
    16501 tgctcggcct gggtgatctc catcccgagc agcccgccga cccgggcggc tcgcagggtc
    16561 agctcgcgcg gggccgggat ctcggcggcg acgtcgatcg tgcccggcac cagcttcgcc
    16621 ccgcagagct caaccatcag cttcgaggcg accgcctggg cgcgcaggca gaggtccggg
    16681 tgcagctgct tctcgaaccg cgaggaggcc tccgagcgca gcccgagcag gcgcgaggtg
    16741 cggaggatgt tggtcccgtt ccagttcgcc acctcgagca gcacccgggt ggtcgtctcc
    16801 gagacctcgg agacctggcc gcccatgatc ccggcgacgc cggtggcgcc gtcgcggtcg
    16861 ctgaccaaca cggtctgcgc gtcgagctcg cgctcgaccc cgtcgagggt cgtcatcctc
    16921 tccccggccg ccgccgtgcg cacggagagc tccccgtccg ggactttgtc gagatcgaag
    16981 gcgtgcagcg gctgcgcggt cagcagcatc acgtaattgg tgatgtcgac gacgttgttg
    17041 atcgggcgct gcccggccgc ggtgagccgc gcctgcagcc agagcggcga gggaccgacc
    17101 gtcacgtcgg tgaagacgcg ggcggtgaag cgcgggcaga gctcgggcac gtcgacggcg
    17161 accgaggcgt agtcctcgac cgccccctcc ccctccgcgg ccgcgtcctc ggcccagggg
    17221 tcggcggcga gcggggcgcc gctgatcgcg tgaacctcgc gggcgacgcc gtgcacgccg
    17281 aagcagtcga cccggttcgg ggtcacctcg atctccagca cgggctcggc cagcggcagc
    17341 acctcgctga gcggcgtgcc ggcgacgagc gcgtcgtcga gaaccatgat cccgtcgtgg
    17401 tcctcgccga cctcgagctc gtcggcggcg aggatcatcc cttccgaggc gaccccgcgc
    17461 agcttcgcct tgcgcagctt ctcgccgccc ggcatccgcg ccccgggcag ggcgacggcg
    17521 acggtctggc cggcaccgac gttgggggcg ccgcagacga tcgtgcgctc ctcgccggag
    17581 ccgtcgtcga ccgtgcagac ccgcagccgg tcggcgtcgg gatgctgctc ggcgctcaga
    17641 accttgccga cgatgaagtt ctcggcgctc ggcggaccga ccacggcgag ccgctcgacc
    17701 tcggtcccgg tcatcaccag gcggtcggcg agctgctcgg ggtcgacgcc cgggtcgcag
    17761 tactcgcgca gccagctcaa aggcaccctc atcagtcgcc cctcacgaga actgctccaa
    17821 catccgcacg tcgttgtcga agaagcggcg caggtcgggg acgccgtgcc gcagcatcgc
    17881 gatccgctcg accccgagcc cgaaggcgaa gccctggaac ttctccgggt cgtagccgtg
    17941 ctgctcgacg aacccgagca cgttcgggtc gaccatgccg gcgccgagga tctcgatcca
    18001 gccctggccc ttgcagaggt tgcagcgctc gccgccctcg agcacgccgc tgccgccgca
    18061 ctggaaacag gagacgtcga cctcgacgct cggctcggtg aaggggaaga agtgcggccg
    18121 caggcgcacc tcgcgctcgt cgccgaagat cgcctggcag agcgccagca gcgtgccctg
    18181 caggtcggcg agggtgatcc cctcggcgat cgccaatccc tcgatctggt ggaacatcgg
    18241 gctgtgggtg gcgtcggaat cgcgccggta gaccttgccg ggcacgatca cgaagatcgg
    18301 cggctgctgc gtctccatcg cccggatctg catcggcgag gtgtgcgtgc gcagcaggac
    18361 ttcggggtgc gactgcacgt agaaggtgtc ctggagcatc cgcgccgggt gcccctcggg
    18421 gtggttgagg gcggtgaagt tgtagtagtc gtgctcgatc tccgggcctt cggcgaccgt
    18481 gtagccgagc ccgaccatga tgtcctcgat ctggcgcgtc gtgcgcgcga tcaggtgcgc
    18541 gtggccgacc ggccgcggcg gcgcgccggg cagggtgacg tcgacccggt cggcgagcag
    18601 cttcgcgtcg agctcggagg cgtcgaggcg cgcgctcgcc tcgtcgatca acgcttccag
    18661 ctgcttgcgc gccttgttgg cggctccgcc gacctgtccc cgttcctcct ggggcagctc
    18721 ggcgatccca cgcaggatcg tggtcagctc ggctttgcgg ccgagatagg agacccgcag
    18781 ctcctccagc gcggcactcg agtcggcggc cgcgatcgcc tcggcgccgg cagccctgat
    18841 cccctcgatc cgatcaagca tcgggctgcc ctccgttcat ggcgggcgac gatatccgct
    18901 gcgcggcaat cgccgccgcc gcggcgacgt tgagcgactc ggcgccgccc cgcagcggga
    18961 tcgtcacctg ggtcccgcag cgggagacga cctcgccggg aagtccctcg cgctcggcgc
    19021 cgaggcagat cgtcgccgtg cccgcgagcg cgccgaggtc ctcgccgccg tgggcgacca
    19081 gcgcggtccg cggctccggg gtctcctcca ccccgccccg caacggcggc tggctgaaga
    19141 ccgagcccat gctcgcccgc accgccttcg gcgagaacgg gtcggcgcac tccggcccga
    19201 gcacgaccga gctgccgatc agcgcgtcgg cggtgcggat gatcgtgccg acgttgccgg
    19261 ggtcggcaac tccgtgcaga taaaggcagg gcgcctcggc ccgctccgcc caggtaagtg
    19321 gccagatcgc gatcacccgc gtcccggagc cgagcgtcga gacgccggca agcaggtccg
    19381 ggtcgacttc ctcaccgtcg atctcggcat cgggcgcggt caggaccagc cgcggcaccg
    19441 ccccggccgc gagcccggct tcgagcaggt cctcgccctc ggtcgcgaac agcccctcgc
    19501 gttcacggtg cttgcgctcg cgcagcttgc ggaccagctt cagcttctcg ttctgggggc
    19561 tctcgatcat cgggtgtctc tcggcgaggt ttctttcggt ccggtcgttt tttctcctgg
    19621 aacgcaaaac gggccgcatc ttgcggcccg cccgggaaag tggagtgtcg gtgtgccgct
    19681 acgcggccgc accctcccgg gctagctcca caaatcggcg gaaggcctcg cggtcgtgca
    19741 ccgcgatgtc ggccagcatc ttccggttca cctcaacttc ggctttgttc aggccgtgca
    19801 tcagttccga gtagctcatg ccctcgagcc gcgcagccgc gttgatccgg gtgatccaga
    19861 gccggcggaa cgtccgcttt ttgttgcggc ggtcgcggta ggcgtacatc cccgaccgct
    19921 gcacctgctc tttggcgaag cggtaggagg agtgcttgcg gccgaagtag cccttcgctt
    19981 ccttcagcac cttgcggcgc ttcttgcgcg cgtggacgga gcgctttacg cgggccatct
    20041 acttgccccc aagcaaggtg cgcacggttt tcgcgtcgtt cggcgagatg tcaaccggtt
    20101 tcgccatctt gcgcttccgc ttcggcgact ttttctccag gatgtggctg gagtaggcgc
    20161 gacggccacg cagtttgccg gtggcggtct ttttgaagcg cttcttagcg ccggaatggg
    20221 ttttcatctt cggcatcggg ttcgctattg aagcagaccc gcgccgccag cggcctggcg
    20281 gggttttccc tgcacctcgt ggcgcccacc cacgcgggta cgctgcgcgc gatgcggatc
    20341 gtctacgtgg tcgggacgcg gcccaatttc gtcaagacgg cgccggtgat cggtgccctg
    20401 cgtgagcgcc tcccggaggg ccgccacgcg atcgtccaca ccggccagca ctacgaccgg
    20461 ctgatgtcgg aggttttcct cgaggagctc ggcgtgcccg cccccgacca catgctcgag
    20521 gtcggctcgg ccagccacgc ggtgcagacc gcgcgcacga tggagcgcct ggagccggtg
    20581 ctgctggagg agcgacccga cctggtgatc gtgcccggcg acgtcaactc gaccctggcc
    20641 gcgaccctga ccgcggtgaa gctgcagatc ccactcgccc acgtcgaggc gggcctgcgc
    20701 agcttcgaca tggcgatgcc cgaggaggcc aaccggatcg tcgccgaccg cttcgccgag
    20761 ttcctcttcc tgcacagcga ggaggcgacc ggcaacctgc gcgccgaagg catccccgag
    20821 gagcgcatgc acttcgtcgg caacacgatg atcgacaccc tggtcgccct tgaagaacgg
    20881 atccgcgccg ccgacgcggc ggccgcgcag gggctgaccc caggcgagta cgtgctcgtc
    20941 accctgcacc ggccggcgct ggtcgacggg ccgctgctcg gcgagacggt ggcgcaactg
    21001 gcggcgttgg ccgagcagat gccggtcgtc ttcccggtcc acccgcgcac ccgcaagatg
    21061 atggagggcg ccggcgccgc cgacagcccc ggcctgcgcc tgatcgagcc gctcggctac
    21121 ctcgacttcc tctcgttgat ggccgacgcc ggcgccgtgc tcaccgactc cggcgggatc
    21181 caggaggaga cgacctacct cggcatcccc tgctttaccc tgcgcgacaa caccgagcgc
    21241 ccgatcacga tcagcgccgg caccaacacc ctgctcgggc tcgacccggc ggcaatcgcc
    21301 ggcatcccca gcgcgctcgc cgcccgcggc aacccaccga ccaagccacc gcccctctgg
    21361 gacggcaacg ccgcgacgcg catcgccgac gtgatcgcca gctgactagg cgtcggcggg
    21421 aggtgaatcg gccgcaggcg cctcggccgc gggtgcgtcg gccgcgggcg cctcggcagc
    21481 gggtgcgtca gtggagtccg tgccggcgat cttgtcgacc tctttgctcg gggccagcaa
    21541 catgctcatg ttgcggcctt cctgcaacgg cgccgactcg atcacggcga tcccaccgag
    21601 atcctcgaag agcttctgca ggaggtcgcg cccgcgctcg gggtgagcct gctcgcgacc
    21661 gcggaacatg atcgtgacct tgaccttgtc gtggtgcttg aggaagcgct cgacgtgacc
    21721 cttcttggtc tcgtagtcgt gggtcgcgat cttcggccgc agcttgattt cgcggatgtt
    21781 gacctgctgc tggtgcttgc gcgcctgctt ctgcttctgc tcctgctcgt atttgtactt
    21841 cgagtagtcg agcaggcggg tgaccggcgg tttcgagttc ggcgcgacct cgaccaagtc
    21901 gaggtcggcg gcctgcgcct ggcgcagcgc ctcggcggtg tcgacgatcc ccagctgctc
    21961 gccgtcggca ccgatcaagc gcacctgcgg gacccggatc cgctcgttga tccgcgtcgt
    22021 gtcgcgctct ggtgggcgcc ggtcgaacct gcgtgggacc ggcactatcg aaggcgggac
    22081 ttcaagagga cgaaacggta ggggtgggca cgc
//
LOCUS       DASRIG010000454         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1769803, whole genome shotgun sequence.
ACCESSION   DASRIG010000454 DASRIG010000000
VERSION     DASRIG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769803"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFB52_12125"
     CDS             <1..750
                     /locus_tag="VFB52_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO07126.1"
                     /translation="PDVFIACCIAPSLGKKYFNQMKENGTVTFMCCTAEEGTEGIDKL
                     VSLPADKIGSGEALANFAVAEKGEELNVLYVNTPDFEVSAEYLEGFEDQVAKFCPECS
                     VDSIEVATEDIGKPAITTAVTGYLQAHPDVNFIGTLFSPLMVGVPAAMAAAGIEDVSI
                     GATANDELSLKAVTEGKENWVAVQNFGQEFGLWGINFAVRTIQGEETGDETLTPEVLV
                     TNKNAEKVLAHPEQWVIPDALEQYEALWGLK"
     gene            839..2431
                     /locus_tag="VFB52_12130"
     CDS             839..2431
                     /locus_tag="VFB52_12130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO07127.1"
                     /translation="MNAEQNPKMASAEPLVRIENLSKTFVGQKALDGVSLQIAPGEVH
                     ALLGQNGCGKSTLIKVLTGYHNPDSGVRFWLDGKEAHLGHGKLIAEDGESIEIRAVHQ
                     DLGLVDRLNAVDNIGLVIGYSSAGAGNIGWRNQAQRTRELLAKVGAGDLDIWRPLDQC
                     DKLHRTQVAIARVLAKWETERGLVILDEPTASLQAQQVDRLFEVIREIRSRGISVLYV
                     SHRLAEIFQIADSLTVLREGKLIDSKPVAETDHDGLISMMLGSTEALAGDAPDPVAAG
                     NGRSPSERPVRLSVSNLSSAELDDLSFEIHEGEILGFAGVVGSGQEELPYLMVGSHRA
                     QSGTVQIGDDEYDLTKMSPRKAKRLGLGFVPADRANQALIMELAVADNMSLATIGDFD
                     RGQWLRKREEATFAVNWGNRLELSPNDPYKAVGLFSGGNQQKVVLGRALSVGKTALIL
                     AEPTAGVDIGAKALIYNYLRKEAAAGTVMIVCSSDVTDLIEVCTRVIALRNGRIAAEL
                     TGDNINEDAMLRSILAPPKERV"
     gene            2431..3504
                     /locus_tag="VFB52_12135"
     CDS             2431..3504
                     /locus_tag="VFB52_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07128.1"
                     /translation="MSSATQQPTAPEEQSQNSGGAVAVPHKSLLSALSFQNISAVYLF
                     GLVVLVFAIWIPDNFLQTDTVAAVLNQNAVPGIVALALVVPLAAGVFDLSVGYTLGAV
                     SVWVAWLLGNTSFSPTICIALGLLMALAIGIANGFIVVVMRVDSFIGTLASGSLLVAL
                     TLIISGNTQLTEGITEDFTSVASTKISDFTLPVFYMLVIAVVMWYFLEHRAIGRQTQA
                     SGLAPEAARLAGIRVDAIKFGSLIVSALLAGVGGILLTATVGGGSPSVGPQYLIPAFA
                     AAFLGATQLKGGLFNAWGTVLAVILLGTVYNGLTLAGVAVWVPYVVQGVVLIAALALA
                     GVKRRSSGGDAEASDELEEAKPA"
     gene            3544..4017
                     /locus_tag="VFB52_12140"
     CDS             3544..4017
                     /locus_tag="VFB52_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07129.1"
                     /translation="MSESATATDLEARVDGLESRFAIEDLIATYAKGFDREDPELLHS
                     IWWEDCVLDFGEAFGTHEGIDAIRTAADGFWQGITWMHHWQATPLIEIDGDRATAYVG
                     LDCLVRSAEEGPVRVGGYYEDVFERRDGVWKFARRMCDIQIWAPDPTYKAETSHS"
     gene            4029..>4547
                     /locus_tag="VFB52_12145"
     CDS             4029..>4547
                     /locus_tag="VFB52_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07130.1"
                     /translation="MSEGSSSGRVEGKVAFITGAARGQGRSHAVRLAEEGADIIAVDL
                     CDEIAGAAYPPATLEDLAQTERAVAALGRRVVTAQADVRDAAALRAVLDPAVAELGGL
                     DFVCANAGIVNLPKPAVELEPELWQQMIDVNLTGAWNTCQVGAPHLIERGGGAIVITS
                     SSAALKGYPSVVN"
BASE COUNT          799 a         1591 c         1475 g          682 t
ORIGIN      
        1 cccgacgtgt tcatcgcctg ctgcatcgcg ccgtctctgg ggaaaaaata tttcaaccag
       61 atgaaagaaa acggcacggt caccttcatg tgctgcacgg ctgaagaagg caccgaaggg
      121 atcgacaagc tggtctcgct gccggcggac aagatcgggt ccggtgaagc cctcgccaac
      181 ttcgcggttg ccgaaaaagg cgaagaactc aacgtcctat atgtgaatac gccggacttc
      241 gaagtcagtg ccgagtactt agaaggcttc gaagatcaag tcgcaaaatt ctgtcccgag
      301 tgctcggtcg actcgatcga agtcgcgacg gaagacatcg gcaaaccggc gatcaccacg
      361 gcggtgaccg gttacctgca ggcccacccc gacgtgaact tcatcgggac gctgttctcg
      421 ccgctgatgg tcggcgttcc cgcggcgatg gccgctgccg ggatcgaaga cgtcagcatc
      481 ggcgcgacgg ccaacgacga actctcgctc aaggcagtca ccgaaggcaa agaaaactgg
      541 gttgcggtcc agaacttcgg tcaggaattc gggctgtggg gcatcaactt cgccgtccgc
      601 acgattcagg gcgaagaaac cggtgacgaa accctcaccc ccgaagtcct cgtcacgaac
      661 aaaaacgcag aaaaagtgct cgcccacccg gaacagtggg tgatcccaga cgcactcgag
      721 cagtacgaag ccctctgggg cctcaaatag caagaccgga ctcgctggcg gccgaggtga
      781 tctcggccgc cagcgttttc acgcacccac caacaccgag acacacaccg aacttgagat
      841 gaacgccgag cagaacccga agatggcgag tgccgagccg ctggtcagga tcgagaacct
      901 ctcgaagacc ttcgtcgggc agaaggcgct tgacggcgtc tccctgcaga tcgccccggg
      961 cgaggtgcac gcgctgctcg gccagaacgg ctgcggcaag tcgaccctga tcaaggtcct
     1021 gaccgggtac cacaaccccg attccggggt tcgcttctgg ctcgacggca aggaggccca
     1081 cctcggccac ggcaagctga tcgccgagga cggcgagtcg atcgagatcc gcgccgtgca
     1141 ccaggacctc ggcctcgtcg accggctcaa cgccgtcgac aacatcggcc tcgtgatcgg
     1201 ctactcgagc gccggcgccg gcaacatcgg ctggcgcaac caggcccagc gcacgcgcga
     1261 gctgctggcc aaagtcggcg ccggcgacct cgacatctgg cgcccgctcg accagtgcga
     1321 caagctgcac cgcacgcagg tggcgatcgc ccgcgtgctc gccaaatggg agaccgagcg
     1381 cggcctcgtg atcctcgacg agccgaccgc ttcgctgcag gcgcagcagg tcgaccgcct
     1441 cttcgaggtg atccgcgaga tccgctcgcg cgggatcagc gtcctctacg tgtcccaccg
     1501 cctcgccgag atcttccaga tcgccgacag cctcacggtc ctgcgcgagg gcaagctgat
     1561 cgacagcaaa ccagtcgccg agaccgacca cgacggcctg atctcgatga tgctcggctc
     1621 gaccgaggcg ctcgccggcg acgcgcccga tccggtcgcg gccggcaacg gccgcagccc
     1681 ctccgagcgg ccggtgcggc tctcggtctc caacctcagc tcggccgagc tcgacgacct
     1741 ctccttcgag atccacgagg gcgagatcct cggcttcgcc ggcgtcgttg gctcgggcca
     1801 ggaggagctg ccgtacctga tggtcggctc ccaccgggcg cagtcgggga ccgtgcagat
     1861 cggtgacgac gagtacgacc tgaccaagat gtcgccgcgc aaggcgaagc ggctcgggct
     1921 cggtttcgtg cccgccgacc gcgccaacca ggccctgatc atggagctcg cggtcgccga
     1981 caacatgtcg ctggcgacga tcggggactt cgacagaggt cagtggctgc gcaagcgcga
     2041 ggaggccacc ttcgcggtca actggggcaa ccggctcgaa ctctcgccca acgaccccta
     2101 caaggcggtc gggctgttca gcggcggcaa ccagcagaag gtcgtgctcg gtcgcgcgct
     2161 cagcgtcggc aagaccgccc tgatcctggc cgagccgacc gccggcgtcg acatcggcgc
     2221 caaggcgctg atctacaact acctgcgcaa ggaggcggcg gcgggcacgg tgatgatcgt
     2281 ctgctcctcc gacgtcaccg acctgatcga agtctgcacg cgcgtgatcg cgctccgtaa
     2341 cggccgcatc gcggccgagc tgaccggcga caacatcaac gaggacgcga tgctccgctc
     2401 gatcctcgca cccccgaagg agagagtctg atgtcgagtg caacccagca gccgaccgcc
     2461 cccgaggagc agtcccagaa ctcgggcggt gccgtcgccg ttccccacaa aagcctgctg
     2521 agcgcgctca gcttccagaa catcagcgcc gtctacctgt tcggcctggt ggtcctcgtc
     2581 ttcgcgatct ggatccccga caacttcctg cagaccgaca cggtcgccgc ggtcctcaac
     2641 cagaacgcgg tccccgggat cgtcgccctc gccctggtcg tgccgctcgc cgccggggtc
     2701 ttcgacctct cggtcggcta cacgctcggc gccgtctcgg tctgggtcgc ctggctgctc
     2761 ggcaacacca gcttctcgcc gacgatctgc atcgccctcg gcctgctgat ggccctggcg
     2821 atcgggatcg ccaacgggtt catcgtcgtc gtgatgcgcg tcgactcgtt catcggcacc
     2881 ctcgccagcg gctcgctgct ggtcgcgctg acgctgatca tctccggcaa cacgcagctg
     2941 accgagggga tcaccgaaga cttcacctcg gtcgcctcga ccaagatcag cgacttcacc
     3001 ctgccggtct tctacatgct cgtgatcgcg gtggtgatgt ggtacttcct cgagcaccgc
     3061 gcgatcggcc gccagaccca ggcctcgggc ctcgcgccgg aggcggcgcg gctcgccggc
     3121 atccgcgtcg acgcgatcaa attcggctcg ctgatcgtct ccgcgctgct cgccggggtc
     3181 ggcggcatcc tgctgaccgc gacggtcggc ggcggctccc cttcggtcgg tccccagtac
     3241 ctgatccccg ccttcgccgc cgccttcctc ggcgccaccc agctgaaggg aggcctcttc
     3301 aacgcctggg gaacggtcct cgccgtgatc ctgctgggca ccgtctacaa cgggctcacc
     3361 ctggccgggg tcgcggtctg ggtcccgtac gtggtccagg gcgtcgtgct gatcgcggcg
     3421 ctggcgctgg cgggtgtgaa gcggcgctcc tccgggggcg acgccgaggc gtccgacgag
     3481 ctcgaagagg cgaagcccgc atagggcccg ccaaagggaa tccagggaaa caaggaggag
     3541 cagatgtccg agtccgcaac cgcaaccgat ctcgaggctc gcgtcgacgg gctcgagagc
     3601 cgcttcgcga tcgaagacct gatcgcgacc tacgccaagg gcttcgaccg cgaagacccc
     3661 gagctgctgc actcgatctg gtgggaggac tgcgtcctcg acttcggcga ggccttcggg
     3721 acccacgaag ggatcgacgc gatccgcacc gccgccgacg gcttctggca gggcatcacc
     3781 tggatgcacc actggcaggc gacgccgctg atcgagatcg atggcgaccg cgccaccgcc
     3841 tacgtcggcc tcgactgcct ggtccgctcg gccgaagaag gcccggtccg ggtcggcggc
     3901 tactacgagg acgtcttcga gcgccgcgac ggggtctgga agtttgcccg gcggatgtgc
     3961 gacatccaga tctgggcgcc cgatccgact tacaaagccg agacttccca ctcctagggg
     4021 aagcgaggat gagcgagggc agcagcagcg gccgggtcga gggcaaggtc gccttcatca
     4081 ccggcgccgc tcggggccag ggccgcagcc acgcggtgcg cctggccgaa gagggggcgg
     4141 acatcatcgc cgtcgacctc tgtgacgaga tcgccggggc ggcctacccg ccggcgacgc
     4201 tcgaggacct cgcccagacc gagcgcgccg tggccgcgct cgggcgtcgt gtcgtcaccg
     4261 cgcaggcgga cgtgcgcgac gccgcggcgc tgcgcgcggt gctcgacccg gcggtcgccg
     4321 agctcggcgg cctcgacttc gtctgcgcca acgccgggat cgtcaacctg ccgaaaccgg
     4381 cggtcgagct ggagccggag ctctggcagc agatgatcga cgtcaacctg accggggcct
     4441 ggaacacctg ccaggtcgga gcgccgcacc tgatcgagcg cggcggcggc gcgatcgtga
     4501 tcaccagctc ctcggcggcg ctgaagggct acccgagcgt ggtcaac
//
LOCUS       DASRIG010000455         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2028319, whole genome shotgun sequence.
ACCESSION   DASRIG010000455 DASRIG010000000
VERSION     DASRIG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028319"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFB52_12150"
     CDS             complement(<1..363)
                     /locus_tag="VFB52_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZO07131.1"
                     /translation="MEGQLFETGDDSAAQPAVPADAPLAVRMRPRSLDELIGQEHVVG
                     PGSALRAAIESGKPHSAILYGPPGAGKTTLARIAASGAEGAFEEESAVNAGKAEIRAV
                     IERARERRRASGRPTILFL"
     gene            378..1973
                     /locus_tag="VFB52_12155"
     CDS             378..1973
                     /locus_tag="VFB52_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO07132.1"
                     /translation="MESATAVQESLSTLESFSPATGELVGTVPTIAPEQVQAVVDDVA
                     RVQPAWAELRPKTRAEYMRRAALALLDDIDEVAELLVREQGKPRVEAYTMELLPTVDA
                     LHYCAKQGPKILADEKVKMTQAFTLSKSGHFSYEPIGVVGVIAPWNYPWSIPFGEVAI
                     ALMAGNGVVLKPASLTPLLGEKIREVFEKGGLPEGLVRVVHGGGAVGEALAKSSVGKV
                     FFTGSVEVGRHVGEVCAAALKGSVLELGGKDPMIVCADADLDNAISGAIWGGFANAGQ
                     TCSGIERVYVERAVADRFIEGVAREAAAMQLGNPLEWDTEIGPMTSEGQYEIVVELIE
                     DAVASGATKLCGGPTEVPGLPGKFIAPTVLTGVTHSMRIMREEIFGPVLPIVVVDSEQ
                     QAIELANDSEFGLGASVWTKDRQRGERIARRIESGMVWINDHSFSHGACQCAWGGVKD
                     SGLGRSHSKFGFYECVNIKMNAWEPGLARDFWWHPYDRTLGEAVKASAKILYGKGETR
                     TKALRDGVGPLLGVGRRTLRKRR"
     gene            complement(1978..>2196)
                     /locus_tag="VFB52_12160"
     CDS             complement(1978..>2196)
                     /locus_tag="VFB52_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07133.1"
                     /translation="RIVVLKQKGNGILSRVESKPNGNWKADLDELNENIKIPAKVFAE
                     VKPVTQGTAGPIYKCLAAKSKTVEIAGG"
BASE COUNT          326 a          760 c          770 g          340 t
ORIGIN      
        1 gaggaagagg atcgtcggcc ggccgctggc gcggcggcgc tcgcgcgccc gctcgatcac
       61 cgcgcggatc tcggctttgc cggcgttgac cgccgactcc tcctcgaagg ccccctccgc
      121 cccactcgcc gcgatccgcg ccagcgtcgt cttgccggcc ccgggcggcc cgtagaggat
      181 cgcgctgtgc ggcttgcccg actcgatcgc cgcgcgcagc gccgagcctg gcccgaccac
      241 gtgctcctgc ccgatcagct cgtcgagcga gcgcggccgc atccgcaccg ccaacggcgc
      301 gtcggccggc accgcgggct gcgccgcgga gtcgtctccg gtttcgaaca gttgaccctc
      361 caacgtaaaa ttggagtatg gagagcgcca cggcagttca agagtccctg tccaccctgg
      421 agtcgttcag cccagccacg ggggagctgg tcgggacggt tccgacgatc gcgccggagc
      481 aggtgcaggc ggtggtcgac gacgtcgccc gcgtacagcc ggcctgggcc gagctgcgcc
      541 cgaagacgcg cgccgagtac atgcgccgcg ccgcgctggc gctgctcgac gacatcgacg
      601 aggtcgccga gctgctggtc cgcgagcagg gcaagccgcg ggtcgaggcc tacacgatgg
      661 agctgctgcc gaccgtcgac gcgcttcatt actgcgcgaa acaagggccg aagatcctcg
      721 ccgacgagaa ggtgaagatg acccaggcct tcacgctctc caagagcggt cacttctcgt
      781 atgagccgat cggcgtggtc ggggtgatcg cgccctggaa ctacccctgg tcgatcccct
      841 tcggcgaggt cgcgatcgcg ctgatggcgg gcaacggcgt cgtgctcaag ccggccagcc
      901 tgaccccgct gctgggcgag aagatccgcg aagtgttcga gaaggggggc ctgcccgagg
      961 gcctggtccg ggtcgtccac ggcggcggcg cggtcggcga ggcgctggcc aagagcagcg
     1021 tcggcaaggt cttcttcacc ggctcggtcg aggtcggccg ccacgtcggc gaggtctgcg
     1081 cggcggcgct caagggctcc gtgctggagc tcggcggcaa ggacccgatg atcgtctgcg
     1141 ccgacgccga cctcgacaac gcgatctccg gcgcgatctg gggcggcttc gccaacgccg
     1201 gccagacctg ctcggggatc gagcgggtct acgtcgagcg ggcggtcgcc gaccgtttca
     1261 tcgagggcgt cgcccgcgag gcggcggcga tgcagctcgg caacccgctc gagtgggaca
     1321 ccgaaatcgg cccgatgacc tccgagggcc agtacgagat cgtcgtcgag ttgattgagg
     1381 acgcggtcgc ctccggggcg acgaagctct gcggcggccc gaccgaggtc cccggcctac
     1441 ccggcaagtt catcgccccg accgtgctca ccggcgtcac ccactcgatg cggatcatgc
     1501 gcgaggagat cttcggcccg gtcctgccga tcgtcgtcgt cgactccgag cagcaggcga
     1561 tcgagctcgc caacgactcc gagttcgggc tcggcgcctc ggtctggacc aaggaccgcc
     1621 agcgcggcga gcggatcgcg cgccggatcg agtccggcat ggtctggatc aacgaccact
     1681 cgttcagcca cggcgcctgc cagtgcgcgt ggggcggggt caaggactcc ggcctcggcc
     1741 gctcgcactc caaattcggc ttctacgagt gcgtgaacat caagatgaac gcctgggagc
     1801 cgggcctggc ccgcgacttc tggtggcacc cctacgaccg caccctgggc gaggcggtca
     1861 aggcctcggc gaagatcctc tacggcaagg gcgagacccg aacgaaggcg ctgcgcgacg
     1921 gcgtcggccc actgctggga gtgggccgac gcacgctgcg caagcgtcgc tagaggatca
     1981 gccgccggcg atttcgacgg tcttggactt cgccgctagg cacttgtaga tcgggcccgc
     2041 ggtcccctgg gtgaccggct tcacttcggc gaagaccttc gccgggatct tgatgttttc
     2101 gttcagttcg tcgaggtcgg ccttccagtt gccgttcggc tttgattcga cccggctgag
     2161 gatgccgttg cccttctgct tgaggacgac gatccg
//
LOCUS       DASRIG010000456         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_979496, whole genome shotgun sequence.
ACCESSION   DASRIG010000456 DASRIG010000000
VERSION     DASRIG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979496"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="gpmI"
                     /locus_tag="VFB52_12165"
     CDS             <1..1029
                     /gene="gpmI"
                     /locus_tag="VFB52_12165"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZO07134.1"
                     /translation="KGYLEELERWLRAAGRVGTVGGRYYAMDRDTRWERTKLAYDAIV
                     HAQGLGAASSAEAIEGAYAREETDEFVVPTVIGDYDGAAAGDVALFINFRPDRARQMT
                     RALAEPGFDEFSRAGAATVDLTTMTQYRKGWPYPVAFPEDRPKTTLAETISEAGGLQL
                     HVAETEKYAHVTYFFNGGREAELAGEEHCLVESPRDVPTYDHKPEMAAEDAADAFVSR
                     WREGTYRFGIINFANPDMVGHTGVIPAAVKAVETVDTQLGKVVEAVLGNGGACIVTAD
                     HGNCDHMLEPDGSPNTAHSLNPVPLIVTAEGLDLRDGGILADVAPTALQLLGIPQPAA
                     MTGRSLIA"
     gene            1026..2231
                     /gene="tgt"
                     /locus_tag="VFB52_12170"
     CDS             1026..2231
                     /gene="tgt"
                     /locus_tag="VFB52_12170"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZO07135.1"
                     /translation="MRSEPQRLRFEISARDGTARAGTIQTAHGPIETPAFIPLATKGT
                     VRGLESAEVAGLGYELILGNTYHLFVSPGSERIEAAGGLHGFMGWERALITDSGGFQV
                     FSLAHGGVANEVKGSGRASGEAPRAKVEITEEGARFRSYRDGSELFISPEVSMSVQAS
                     LGSDIALVFDECTPFHADREYTARSTERTHRWLGRCLDWHAENGPERQAVFGIVQGGV
                     YEDLRRESAQVVSEAAVDGLAIGGTLGRDKEEMASVLAMTAPLLPEEAAKHLLGIGEV
                     DDLLGGIALGLDVFDCAVPTRLARHGVALAPSPETRWRLDLRKGRWVGDRDPMVPGCP
                     CPACQRHDRDYISYLSRAEELTAVRLIVLHNLVYMRELTQHARRAISEGRFNAYRASV
                     LAGSAPWDA"
     gene            complement(2387..3616)
                     /locus_tag="VFB52_12175"
     CDS             complement(2387..3616)
                     /locus_tag="VFB52_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZO07136.1"
                     /translation="MEAGTLSGRYETGDRLGSGGMSNVYMATDKILERTVAVKILAEH
                     LSDDERFVARFKREALAVAKLIHPNIVQVYDTGVDEGRHYIVMEYVDGRSGAQILQRQ
                     GPVEPETAADIGIQACAGLDYAHRRGIIHRDVKPGNLMVVGGPVGGGEMTVKLTDFGI
                     ARAIEQTRITQVGSVVGTAAYLAPEQVRGEEATPATDVYALGVVLYQFLTGRLPYEGS
                     TLAELAVRQQNETPLPPSTYNDEVPEALGASVLRALEGDPNRRYASADELSNGLQMGL
                     EGTDVSLPPAEGATPTRVLGGDTAATRHLERDTGPTEFRPAPSQTRRPEPRIAPAPAQ
                     AAPSRASAAPKRSGFSRFVRFVLALVALVLVAAAVATAVILATDKAASVHIREVAGDT
                     VDKVTEEFKDLVDKNTE"
     gene            complement(3616..4197)
                     /locus_tag="VFB52_12180"
     CDS             complement(3616..4197)
                     /locus_tag="VFB52_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07137.1"
                     /translation="MGRSRALPLALALALAAALTLSACGSDDGAELLPGTTAAAITEN
                     LESVEQLAAEGDCTGATDAAEEVSLQVDELTGVDKELKQALEQGATKLNEVVDECDEA
                     PEEDEEETISTEAEEPSADEQKEEEREQKAQEKADKEAEKEQQKEEKEQEKESPPPVE
                     PPEKEEKTPPENEEDGGTPSGGVSPGVSAEGGE"
     gene            complement(4251..4676)
                     /locus_tag="VFB52_12185"
     CDS             complement(4251..4676)
                     /locus_tag="VFB52_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZO07138.1"
                     /translation="MKRADIDLYHASPRDPVWEYVLSIEQAEAGFDAQDERIALIGHS
                     HVALFFNRPEGARPGYSHGAQAGDGALVHLDDGTWLLNPGSVGQPRDGDPRAAWLELD
                     TEELTARYHRIPYEIEAAAGAIRKAGLPEPLAERLFVGR"
BASE COUNT          640 a         1661 c         1664 g          779 t
ORIGIN      
        1 aagggctacc tcgaggaact cgagcgctgg ctgcgcgcgg ccggccgcgt cggcacggtc
       61 ggcggccgct actacgcgat ggatcgcgat acccgctggg agcggaccaa gctcgcctac
      121 gacgcgatcg tgcacgcgca ggggctgggg gcggcgagct cggcggaggc gatcgagggc
      181 gcgtacgcgc gcgaggaaac cgatgagttc gtcgtgccga ccgtgatcgg cgactacgac
      241 ggcgccgccg ccggcgacgt cgcgctgttc atcaacttcc ggcccgatcg ggcccggcag
      301 atgacccggg cgctggccga acccggcttc gacgagttct cgcgggccgg tgcggcgacc
      361 gtcgacctga ccacgatgac gcagtaccgc aaaggctggc cttacccggt cgcctttccc
      421 gaggaccgcc cgaagacgac cctggcggag acgatctccg aagccggcgg gctgcagctg
      481 cacgtcgccg agaccgagaa gtacgcgcac gtcacctact tcttcaacgg cggccgcgag
      541 gccgagctgg ccggcgagga gcactgcctg gtcgaatcgc cgcgcgacgt gccgacctac
      601 gaccacaagc cggagatggc cgccgaggac gccgccgacg ccttcgtcag ccgctggcgc
      661 gagggcacct accggttcgg gatcatcaac ttcgccaacc ccgacatggt cggccacacc
      721 ggcgtgatcc cggccgcggt gaaggcggtg gagacggtcg acacccagct cggcaaggtg
      781 gtcgaggcgg tgctcggcaa cggcggcgcc tgcatcgtca ccgccgacca tggcaactgc
      841 gaccacatgc tcgagcccga cggctcgccc aacaccgccc actcgctgaa cccggtgccg
      901 ctgatcgtca ccgccgaggg gctggacctg cgcgacggcg gcatcctcgc cgacgtcgcc
      961 ccgaccgccc tgcagctgct cgggatcccg cagccggcgg cgatgaccgg ccgctcgctg
     1021 atcgcgtgag gtcagagccg cagcgcctgc ggttcgagat ctccgcccgc gacggcaccg
     1081 cccgcgccgg gacgatccag actgcgcacg ggccgatcga gacgcccgcc ttcatcccgc
     1141 tggcgaccaa gggcacggtg cgggggctgg agtcggccga ggtcgccggg ctcggctacg
     1201 agctgatcct cggcaacacc taccacctgt tcgtctcccc ggggtcggag cggatcgagg
     1261 ccgccggtgg gctgcacggc ttcatgggct gggagcgggc gctgatcacc gactcgggcg
     1321 gtttccaggt cttctcgctc gcccacggcg gggtcgccaa cgaggtcaag gggtcgggac
     1381 gggcgtccgg cgaggcgccg cgagcgaagg tcgagatcac cgaggagggc gcgcgcttcc
     1441 ggtcctaccg cgacggcagc gagctgttca tctcgcccga ggtctcgatg agcgtgcagg
     1501 cctcgctcgg ctccgacatc gccctggtct tcgacgagtg cacgcccttc cacgccgacc
     1561 gcgagtacac cgcgcgctcg accgagcgca cgcaccgttg gctcggccgc tgcctcgatt
     1621 ggcacgcgga gaacggcccc gagcgccagg cggtcttcgg gatcgtccag ggcggcgtct
     1681 acgaggatct gcggcgcgaa tcggcgcagg tggtctccga ggcggcggtg gacggcctcg
     1741 cgatcggcgg cacgctcggc cgcgacaagg aggagatggc gagcgtgctg gcgatgaccg
     1801 caccgctgct gcccgaggag gcggccaagc acctgctcgg gatcggcgag gtcgacgacc
     1861 tgctcggcgg gatcgcgctc ggcctcgacg tcttcgactg tgcggtgccg acccgcctgg
     1921 cccgccacgg cgtcgccctg gcgccgagcc cggagacccg ctggcgcctc gacctgcgca
     1981 agggccgttg ggtcggcgac cgcgacccga tggtccccgg ctgcccctgc ccggcctgcc
     2041 agcgccacga ccgcgactac atctcctacc tctcccgcgc cgaggagctg accgccgtcc
     2101 gcctgatcgt cctccacaac ctcgtctaca tgcgcgagct cacgcagcac gcgcgtcgcg
     2161 cgatctccga gggccgcttc aacgcgtacc gagcctcggt gctcgccggc tctgctccct
     2221 gggacgcata aagaaggccg aatatcgcac ccgcctgtgg ggagggatcg ggtacccctc
     2281 ggaacgtctt gtactttttc gccgccccca actcggacgg ctctatcccg ccgacgaaaa
     2341 agttaagtgc agaggggtac ccgatccctc cacgcaaacg tctgagctat tcggtgtttt
     2401 tatcgaccag gtccttgaat tcttcggtga ccttgtcgac ggtgtcgccg gcgacttcgc
     2461 ggatgtggac gctggccgcc ttgtcggtgg cgaggatcac cgcggtcgcc accgcggcgg
     2521 cgaccaggac cagggcgacg agcgccagga cgaagcggac gaagcgtgag aacccggagc
     2581 gtttgggcgc ggccgaagcg cgcgagggcg ccgcttgcgc gggcgccggg gcgatccgcg
     2641 gttcggggcg gcgggtctgc gacggggccg ggcggaactc ggtcgggccg gtgtcgcgct
     2701 cgaggtgacg ggtggcggcg gtgtcgccgc cgagcacccg ggtcggggtc gcgccctccg
     2761 cggggggcag cgagacgtcg gtcccttcga ggcccatctg cagcccgttg gagagctcgt
     2821 cggcgctcgc gtagcgccgg ttcgggtcgc cctccaaggc gcgcagcacc gacgccccga
     2881 gcgcctccgg cacctcgtcg ttgtaggtgc tcgggggcag cggcgtctcg ttctgctggc
     2941 gcactgccag ctcggccagg gtcgagccct cgtagggcag gcggccggtg aggaactggt
     3001 agagcacgac gccgagtgcg tagacgtcgg tcgccggggt cgcctcctcg ccgcgcacct
     3061 gctcgggcgc caggtaggcg gcggtgccga ccaccgagcc gacctgggtg atccgggtct
     3121 gctcgatcgc ccgtgcgatc ccgaagtcgg tcagcttcac ggtcatctcg ccgccgccga
     3181 ccgggccgcc gacgaccatc aggttgcccg gcttgacgtc gcggtggatg atcccgcggc
     3241 ggtgcgcgta gtcgaggccg gcgcaggcct ggatcccgat gtcggccgcg gtctccggtt
     3301 cgaccggccc ctggcgctgc aggatctgcg cgcccgagcg cccgtcgacg tactccatga
     3361 cgatgtagtg gcggccctcg tcgacgccgg tgtcgtagac ctggacgatg ttcgggtgga
     3421 tcagcttcgc gaccgccagc gcctcgcgtt tgaagcgggc gacgaagcgc tcgtcgtcgg
     3481 agaggtgctc ggcgaggatc ttcaccgcga ccgtgcgctc gaggatcttg tcggtcgcca
     3541 tgtagacgtt cgacatcccg ccggagccga gccggtcacc ggtttcgtag cggccagaga
     3601 gcgtgcctgc ctccatcatt caccgccttc cgcggagacg ccaggggaga ccccacccga
     3661 gggcgtgccg ccgtcttctt cgttttcagg cggagttttt tcttcttttt cgggcggttc
     3721 gaccggcgga ggcgattctt tttcctgttc tttttcttct ttctgctgtt ccttttcagc
     3781 ttccttgtcg gccttttcct gcgccttctg ttcccgttct tcttccttct gttcgtccgc
     3841 gctcggttct tctgcttcgg tcgagatcgt ttcttcttcg tcttcttccg gcgcttcgtc
     3901 gcattcgtcg acgacttcgt tgagcttggt ggcgccctgt tcgagcgcct gcttcagttc
     3961 tttgtcgacc ccggtcagtt cgtcgacctg gagcgagacc tcttcggcgg catcggtggc
     4021 gcccgtgcag tcgccttcgg cggccagctg ttcgacgctt tcgaggtttt cggtgatcgc
     4081 cgccgcggtc gttcccggca gcagctcggc tccgtcgtcg ctgccgcagg cagagagcgt
     4141 cagcgccgcc gcgagggcga gcgcaagggc aagcggaagg gctctcgaac gacccacgaa
     4201 ttcgagtcta gagaacgctc ccgaaggcgg attttccctg catttgcgca tcagcggccg
     4261 acgaagagcc gttctgcgag cggctccggc aggcccgcct tgcgaattgc ccccgccgct
     4321 gcctcgatct cgtaggggat gcggtggtag cgcgcggtca gctcctcggt gtcgagctcc
     4381 agccaggcgg cccgggggtc accgtcgcgc ggctggccga cgctgccggg attgagcagc
     4441 caggtgccgt cgtcgaggtg gacgagcgcg ccgtcgcccg cctgggcgcc gtgcgagtag
     4501 ccgggccgcg ccccctccgg gcggttgaag aaaagcgcca cgtgcgagtg cccgatcagc
     4561 gcgatccgct cgtcctgcgc atcgaagccg gcttccgcct gttcgatcga gagcacgtac
     4621 tcccagacgg ggtcgcgggg ggaggcgtgg tagaggtcga tgtctgcgcg cttcatcgca
     4681 ggctccaaac ccctcaggta gtccaaggtc gcggcgctgg ccgcgccgcg ggtccactcg
     4741 accg
//
LOCUS       DASRIG010000457         8957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_452540, whole genome shotgun sequence.
ACCESSION   DASRIG010000457 DASRIG010000000
VERSION     DASRIG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452540"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFB52_12190"
     CDS             <1..639
                     /locus_tag="VFB52_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO07139.1"
                     /translation="PESIRTSGAGGATIVTGHTADDQVETILYRLASSPSRRALLGMR
                     PHDGRLARPLLGTNREEITAYCEERRLAWRDDASNADQNFARNRIRHGLLPELEKVHP
                     AAAANVLRTAALLRDEAEVLDALVGAELDGSGEAPRGAITLARLNELPPALRRLVVQR
                     LADRAGGRPIAGAANHAEEVAALRRTGTALLDLGGGVRAVVERGVLRAERDK"
     gene            710..1243
                     /gene="hpt"
                     /locus_tag="VFB52_12195"
     CDS             710..1243
                     /gene="hpt"
                     /locus_tag="VFB52_12195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZO07140.1"
                     /translation="MPKNERGIGETLVASEELQARVAQLGEEISRDYAGRELILVGVL
                     KGAVLFLADLMRHIDVPCEIDFMAVSSYGSQTDSSGVVRILKDLDTSIEGRDVLIVED
                     IIDSGLTLQYLMRNLRARNPASLEVCALLTKPERLRVDLSPRYIGFEIPNRFAIGYGL
                     DHAQRYRNLGYVAALTD"
     gene            1279..3231
                     /gene="ftsH"
                     /locus_tag="VFB52_12200"
     CDS             1279..3231
                     /gene="ftsH"
                     /locus_tag="VFB52_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZO07141.1"
                     /translation="MRRFFKSAAFPILLVVILAFIAQRVITNDNGPPAPPYNELIKPE
                     TGLIADGKVEKVTINTKDNSLDVDLKNGEKFSTGYPLDTEQNLTDVLVENNVETKVND
                     SGGSGLLSLLTYILPFVLFLGFWIFLMNQMQGGGSRVMNFGKSKAKRMSVDAPKITFR
                     DVAGADEAVQELGEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARAVAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGMGGGH
                     DEREQTLNQLLVEMDGFEMKDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRKG
                     RKKILEVHTRGKPLGRSIDLDTLAGQTPGFTGADLANLINEAALLTARSGKREITMKE
                     LEEGIMRVIAGPEKKTRVMSEKERLVTAYHEMGHAIVGHLLENTDPVHKVSIISRGQA
                     LGYTISLPTEDKFLTTRAELTDTMAMTLGGRAAEEIIFGEITTGASNDLEKVTETAKQ
                     MVMRFGMSERLGPRVFGHDRGQPFLGREFSSEPDYSDEIAREIDDEIRRIVESAHQTA
                     RKILNENHEELDRISKLLLERETIDADQFVALLEGKSEAEVFADDDEEKAEPPAAEEP
                     EPEKAAREGARPAPRPRPGFAGGDAS"
     gene            3242..4021
                     /gene="folP"
                     /locus_tag="VFB52_12205"
     CDS             3242..4021
                     /gene="folP"
                     /locus_tag="VFB52_12205"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZO07142.1"
                     /translation="MAKLMGVVNVTPDSFSDGGLYLDPEAAIAHGEELLEAGAEILDV
                     GGESTRPGADPVAEDEERRRVLPVIEGLAGATISVDTSKAAVAAAALDAGAAIVNDVT
                     ALRGDPGMVALCAEHEATVILMHMLGEPRTMQDEPTYGDVVAEVHDFLAERLEFALAA
                     GIEEERVWLDPGIGFGKTADHNLELLRRLPELTALGRPLVIGTSRKSFIGKVDGSAAR
                     ERLGGTIASTVLAAQAGADVLRVHDVAEVGQALTVATAIHG"
     gene            4032..4454
                     /gene="folB"
                     /locus_tag="VFB52_12210"
     CDS             4032..4454
                     /gene="folB"
                     /locus_tag="VFB52_12210"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZO07143.1"
                     /translation="MEPHGPDNGIDSSAEIELRGLSIYTHHGVSDAEQEVGQRLEFDI
                     SFDVPDCDAVLTDRLEDTVDYAQVCDIVALAATERSYRTLERLAQVVGERLMEQYGSE
                     SVRVRAAKPEPPLPLAIQEVAVEVTQERGADEDPGPEN"
     gene            4454..4948
                     /gene="folK"
                     /locus_tag="VFB52_12215"
     CDS             4454..4948
                     /gene="folK"
                     /locus_tag="VFB52_12215"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZO07144.1"
                     /translation="MRVGYLGLGSNVGDSAGHLRAAIELLGERGVEVEAVSSLYVTEP
                     VGEILDQPDFLNAAVRIRTELDPEQLLDACKAVEAERGRAFDLPRHSPRPLDVDLLLL
                     GEIELSTERLTLPHREVATRRFVLAPLLELDPEMTLPDGTRLADALAALPDDEQRVER
                     RGSL"
     gene            complement(4945..5700)
                     /locus_tag="VFB52_12220"
     CDS             complement(4945..5700)
                     /locus_tag="VFB52_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZO07145.1"
                     /translation="MSARLFVIPASHPSITIELMLAHKGIEYKRTDLLPVISKGVLRA
                     AGFPGVTVPALKIDGTKVQGSRPIARELERLRPEPPLFPSDPAQRAAVEEAERFGDEE
                     LQHPIRQILWWGMKRDHAPLRSYSEGAKIGMPIGLAVKTAAPIVALSARFNEADDEHA
                     RAALAELPSLLQKVDDLIAAGTINGEQLNAADFQIATSLGLAMTLDDLRPAIENRPAG
                     ALAKRVVPNYPGKMPPILPPAWLEPLRGEPATA"
     gene            5784..6548
                     /locus_tag="VFB52_12225"
     CDS             5784..6548
                     /locus_tag="VFB52_12225"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZO07146.1"
                     /translation="MLLAVDIGNTQTHLGAFQGEELIEHWRFQTRSGATGDELAARID
                     GLFALCEMSFTEVDAVCVSSVVPPLGGEFERLSERYLEADCLTIGPGVKTGMPIRIEN
                     PLEVGADRLVNAIAAYDRFGEACVSVDFGTGINIDAVSAEGEYLGGAIGPGLAPSLTA
                     LIDRAARISRIELEEPQEAIGRSTRAAINSGFIFGFAGLIDGIVRRVEKELPNPRLLA
                     TGGYASAVVPFTETIDEVDDLLTLKGLRLIHERNQA"
     gene            6529..7533
                     /locus_tag="VFB52_12230"
     CDS             6529..7533
                     /locus_tag="VFB52_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZO07147.1"
                     /translation="MSATRPELTDPFEIGGIAIANRVLLAPLAGIGNWFVRLQAKRHG
                     AGLAVSEMVSSFALHYRNERTLRDLMRIHPDEHPVSLQLFGHDAEVMRSGAEIAAGAG
                     ADLIDINMGCPVPKVRKTGAGAQLLRDPELALALACAAKEGSGLPVTVKLRSGIEAGD
                     RSGFELAVRLAEEAGVAAIAFHPRAATKGHKGQPDYALTRELVERVEVPVIVSGGLSS
                     ARAARAAYEESGADAVMIARGSLGNPWVFEELTGQRIEPPSRDEIVAELLWVIDRAEE
                     HLGTERAERYLRKFYPWYSERLDAPPALVDELHRSADLDRARNLIRSLGTPAGLTSTA
                     "
     gene            7673..8152
                     /gene="greA"
                     /locus_tag="VFB52_12235"
     CDS             7673..8152
                     /gene="greA"
                     /locus_tag="VFB52_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZO07148.1"
                     /translation="MSRQAVVTPEGLEKLKAEIEELSTVKRREVAERIKEAREFGDIS
                     ENAEYDDAKNEQALLENRIAQLEERLRRATVIDEKALSTDEVGFGSIVHVKDQKTGDS
                     QKFQIVGSTEANPLEHKLSNESPIGKALVGKKRNDVVTVEVPRGPKKKFKITKIEKA"
     gene            8149..>8957
                     /locus_tag="VFB52_12240"
     CDS             8149..>8957
                     /locus_tag="VFB52_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZO07149.1"
                     /translation="MTEELSDVLRDRREKLGRIRAAGIDPFPHEFSQREDIAEVRAAH
                     EGLAPGIETDSRHRVAGRLVARRGHGKACFLDLRDGSGQVQLHAREDLLGPEAYELLV
                     DLDLGDVIGVEGTAMATRRGGELSLAIDHWRLLSKSLRPPPDKFHGLGDTETRLRRRE
                     LDLIANEESRRVFQVRARTITEIRRWLDEHGFVEVETPVLQPLYGGALARPFVTHHNQ
                     LDRDFYLRIATELYLKRLVVGGIDRVYELGKDFRNEGVSHKHNPEFTMLEW"
BASE COUNT         1484 a         3127 c         3048 g         1298 t
ORIGIN      
        1 ccggagagca tcaggaccag cggcgccggc ggggcgacga tcgtcaccgg ccacaccgcc
       61 gacgaccagg tcgagacgat cctctaccgg ctggcctcct cgccgagccg ccgggcgttg
      121 ttggggatgc gaccgcacga cggccggctg gcccggcccc tgctcggcac gaaccgggag
      181 gagatcaccg cctactgcga ggagcgccgg ctggcctggc gagacgacgc cagcaacgcg
      241 gaccaaaatt tcgcccgcaa ccggattcgc cacggccttc tgcccgagct ggagaaggtg
      301 cacccggcgg cggcggccaa cgtgctgcgc acggcggcgc tgctgcgcga cgaggccgag
      361 gttctcgacg ctttggtcgg agcggagctc gacggctccg gcgaggctcc gcgcggcgcg
      421 attaccctcg ctcgcctcaa cgagctgcca ccggcgctgc gccgcctcgt cgtccagcgg
      481 ctcgccgacc gcgccggggg ccgcccgatc gccggtgccg ccaaccacgc cgaagaggtc
      541 gccgcgctgc gccgcaccgg aaccgcgctg ctcgacctcg gcggcggcgt ccgggccgtg
      601 gtcgagcgcg gcgtgctgcg cgccgagcgc gataagtagt gtgcccggga gtgcggagcg
      661 accgagcatc catatctgac tccgtgtgag gaagcgaagc acgactgtca tgcctaagaa
      721 cgagcgcggc atcggcgaga ccctggtcgc cagcgaggag ctgcaggcgc gggtcgcgca
      781 actcggcgag gagatcagcc gcgactacgc cggccgcgag ctgattctgg tcggggtgct
      841 gaagggcgcg gtcctcttcc tcgccgacct gatgcgccac atcgacgtgc cctgcgagat
      901 cgacttcatg gcggtctcca gttacggctc gcagaccgac tcctccgggg tcgtgcgcat
      961 cctcaaggac ctcgacacct cgatcgaggg ccgcgacgtg ctgatcgtcg aggacatcat
     1021 cgactccggc ctcaccctgc agtacctgat gcgcaacctg cgcgcccgca accccgcctc
     1081 gctcgaggtt tgcgcgcttc tgaccaagcc ggagcgcctg cgggtcgacc tctcgccgcg
     1141 ttacatcggc tttgagattc ccaaccgctt cgcgatcgga tatggcctcg accacgccca
     1201 gcgctaccgc aaccttggtt acgttgctgc gttgacggac taggcgggaa aacccccctg
     1261 gtacgctgat ttttaccctt gaggcgtttt ttcaaaagcg cggcatttcc gatcctgctg
     1321 gtcgtcatcc tggcgttcat cgcgcagcgc gtgatcacga acgacaacgg cccgccggct
     1381 ccgccctaca acgagctgat caagcccgag accggcctga tcgccgacgg caaagtcgaa
     1441 aaggtcacga tcaacaccaa ggacaactcg cttgacgtcg acctcaaaaa cggcgaaaag
     1501 ttctcgacgg gatatccgct cgacaccgag cagaacctga ccgacgtcct ggtcgaaaac
     1561 aacgtcgaga ccaaggtcaa cgactccggc gggtcagggc tgctctcgct gctcacctac
     1621 atcctgccct tcgtgctgtt cctcggcttc tggatcttcc tgatgaacca gatgcaaggc
     1681 ggcggctcgc gggtgatgaa cttcggcaaa tcgaaagcca agcggatgtc ggtcgacgcg
     1741 ccgaaaatca ccttccgcga cgtcgccggc gctgacgagg cggtgcagga gctcggggag
     1801 atcaaggaat tcctcgagaa cccgaagaag ttccaggcgc tcggcgcccg gatccccaag
     1861 ggcgtccttc tgtacggacc tcccggcacc ggtaagaccc tgctcgcccg cgccgtcgcc
     1921 ggtgaggcgg gcgttccgtt cttctcgatc tccggctcgg acttcgtcga gatgttcgtc
     1981 ggcgtcggcg ccagccgcgt ccgcgacctc ttcgagcagg cgaagcagaa ctcgccctgc
     2041 atcatcttca tggacgagat cgacgccgtc ggccgccacc gcggcgccgg catgggcggc
     2101 ggtcacgacg agcgcgagca gacgctgaac cagctgctgg tcgagatgga cggcttcgag
     2161 atgaaggaca acatcatctt gatcgccgcc accaaccggc ccgacatcct cgatccggcg
     2221 ctgctgcggc ccggccgctt cgaccgccag atcgtcgtcg accgccccga ccgcaaaggc
     2281 cgtaagaaga ttctcgaggt gcacacccgc ggcaagccgc tcggccgctc gatcgacctc
     2341 gacacgctgg ccggccagac ccccggcttc accggtgccg acctcgccaa cctgatcaac
     2401 gaggccgccc tgctgaccgc ccgctccggc aagcgtgaga tcacgatgaa ggagctcgag
     2461 gaggggatca tgcgcgtgat cgccggcccc gagaagaaga cccgggtgat gagcgagaag
     2521 gagcgactgg tcaccgccta ccacgagatg ggccacgcga tcgtcggcca cctgctcgag
     2581 aacaccgacc ccgtgcacaa ggtctcaatc atcagccgcg gccaggcgct tggctacacg
     2641 atcagcctgc cgaccgagga caagttcctc accacccgtg ccgagctgac cgacacgatg
     2701 gcgatgaccc tcggcggccg tgccgccgag gagatcatct tcggcgagat caccaccggc
     2761 gcctccaacg acctcgaaaa ggtgaccgag acggcgaagc agatggtgat gcggttcggg
     2821 atgtccgagc gcctcggccc gcgcgtcttc ggccacgacc gcggccagcc gttcctcggc
     2881 cgcgagttct cttctgagcc cgactactcc gacgagatcg cccgcgagat cgacgacgag
     2941 atccgccgga tcgtcgagag tgcccaccag accgcgcgca aaatcctcaa cgagaaccac
     3001 gaggagctcg accggatctc gaaactgctg ctggagcggg agacgatcga cgccgatcag
     3061 ttcgtcgctc tgcttgaagg caagtcggag gcggaggtct tcgccgacga cgacgaggag
     3121 aaggccgagc ccccggcggc cgaggagccg gagccagaaa aagctgcccg cgagggcgct
     3181 cgcccggccc ccaggccgcg acctgggttc gccggcggcg acgcctccta gccggagcgc
     3241 catggccaag ctgatgggcg tcgtcaacgt gacgcccgac tcgttctccg acggtgggct
     3301 gtatctcgac cccgaggcgg cgatcgcgca cggggaggag ttgctcgagg cgggagccga
     3361 gatcctcgac gtcggcggcg aatcgacccg gccgggggcc gacccggtcg ccgaggacga
     3421 ggagcggcgc cgggtcttgc cggtgatcga gggcctcgcc ggggcgacga tctcggtcga
     3481 cacgtccaag gcagcggtcg ccgccgcggc gctcgacgcc ggggcggcga tcgtcaacga
     3541 cgtcaccgcg ctgcgcggtg accccgggat ggtcgcgctc tgcgccgaac acgaggcgac
     3601 cgtgatcctg atgcacatgc tcggcgagcc gcgcacgatg caggacgaac cgacctacgg
     3661 cgacgtcgtc gccgaggtgc atgacttcct cgccgagcgg ctcgagttcg cgctcgcggc
     3721 cgggatcgag gaggagcggg tctggctcga ccccgggatc gggttcggca agaccgccga
     3781 ccacaacctc gagctgctgc gccggctgcc cgagctgacc gcgctcggcc ggccgctggt
     3841 tatcggcacc tcgcgcaaga gtttcatcgg caaggtcgac ggctcggcgg cgcgcgagcg
     3901 gttgggcggc acgatcgcca gcacggtgct tgccgcgcag gccggcgccg acgtgctccg
     3961 cgtccacgac gtcgccgagg tcggccaggc cctgaccgtg gcgacggcga tccacggata
     4021 gtctgcaatc catggagcca cacgggcccg acaacgggat cgactccagc gccgagatcg
     4081 agctgcgcgg gctctcgatc tacacccacc acggcgtctc cgacgccgag caggaggttg
     4141 ggcagcggct cgagttcgac atctccttcg acgtgcccga ctgcgacgcc gtgctcaccg
     4201 accggctgga ggacacggtc gactacgcgc aggtctgcga catcgtcgcc ctcgccgcga
     4261 ccgagcgcag ctaccgcacc ctggagcggc tggcgcaggt ggtcggagag cggctgatgg
     4321 agcagtacgg gtccgagtcg gtgcgcgtgc gcgccgccaa gccggagccg ccgttgccgc
     4381 tggcgatcca ggaggtcgcc gtcgaggtga cccaggaacg cggcgccgac gaggaccccg
     4441 ggcccgagaa ctagtgcgcg tcggctacct cggcctcggc tccaacgtcg gcgactcggc
     4501 gggccacctg cgcgccgcga tcgagctgct cggcgagcgc ggggtggagg tcgaggcggt
     4561 ctcctcgctc tacgtgaccg agccggtcgg cgagatcctc gaccagcccg actttctcaa
     4621 cgccgccgtc cgcatccgca ccgagctcga ccccgagcag ctgctcgacg cctgcaaggc
     4681 ggtcgaggcc gagcgcggcc gcgccttcga cctgccccgt cacagccctc gcccgctcga
     4741 cgtcgacctg ctcctgctcg gcgagatcga gctgagcacc gagcgcctca ccctgcccca
     4801 ccgcgaggtc gccacccgcc gttttgtgct cgccccgttg ctcgagctcg acccagagat
     4861 gaccctgccc gacggcaccc gcctcgccga cgcgctcgcg gcgctgcctg acgacgagca
     4921 gcgggtcgag cggcgcggct cgctctaggc ggtcgccggc tcgccgcgca gcggttcgag
     4981 ccaggcgggc ggaaggatcg gcggcatctt gccggggtag ttcgggacca cgcgtttggc
     5041 gagggcgccg gcggggcggt tctcgatcgc ggggcggagg tcgtcgagcg tcatcgccag
     5101 cccgaggctg gtggcgatct ggaagtcggc ggcgttcagc tgctcgccgt tgatcgtgcc
     5161 ggcggcgatc aggtcgtcga ccttctgcag gaggctggga agctcggcaa gggcggcgcg
     5221 ggcgtgctcg tcgtcggcct cgttgaagcg ggcggagagg gcgacgatcg gggccgccgt
     5281 cttcaccgcc aggccgatcg gcatgccgat cttggcgccc tcggagtagc tgcgcagggg
     5341 cgcgtggtcg cgcttcatcc cccaccagag aatctggcgg atcgggtgct gcagctcctc
     5401 gtcgccgaat cgctcggcct cttcgaccgc ggcgcgctgg gcggggtcgg aggggaacag
     5461 cggcggctcg ggccgcagcc gctccagctc gcgggcgatc gggcgcgatc cctgcacctt
     5521 ggtgccgtcg atcttcagtg ccggcacggt gacgccgggg aagccggcgg cgcgcaggac
     5581 gcccttggag atcaccggca gcaggtcggt gcgcttgtac tcgatgccct tgtgggcgag
     5641 catcagctcg atcgtgatgc tcgggtgcga ggcggggatg acaaacagtc gtgcgctcat
     5701 gcgacacgat cctatgcaca tacgcgccgg tttggctact cagccagttt cggcgccggc
     5761 gaccgcgcgg tcataatccg cccgtgctgc tcgccgtcga catcgggaac acgcagaccc
     5821 acctgggagc cttccagggc gaggagctga tcgagcactg gcgcttccag actcgctcgg
     5881 gggcgaccgg cgacgagctc gccgcgcgga tcgacggcct cttcgcgctc tgcgaaatgt
     5941 ccttcaccga ggtcgacgcg gtctgtgtct cctccgtcgt gccgccgctc ggcggggagt
     6001 tcgaacggct cagcgagcgc tacctggaag ccgattgcct gacgatcggg cccggggtga
     6061 agaccgggat gccgatccgg atcgagaacc cgctcgaggt cggcgccgac cggttggtca
     6121 acgcgatcgc cgcctacgac cgcttcggcg aggcctgcgt ctcggtcgac ttcggcaccg
     6181 ggatcaacat cgatgccgtc tccgccgagg gcgagtacct gggcggcgcg atcggtcccg
     6241 gtctcgcgcc ctcgctcacc gccctgatcg accgggcggc ccggatcagc cggatcgaac
     6301 tggaagagcc ccaggaagcg atcggccgct cgacccgggc ggcgatcaac tccggcttca
     6361 tcttcggctt cgccggcctg atcgacggga tcgtccgccg cgtcgaaaaa gaactgccga
     6421 acccgcgcct gctcgccacc ggcggctacg cctcggccgt ggtccccttc accgagacga
     6481 tcgacgaggt cgacgatctg ctgaccttga agggactacg gctgatccat gagcgcaacc
     6541 aggcctgagc taaccgaccc cttcgagatc ggcggcatcg cgatcgccaa ccgggtcctg
     6601 ctggcgccgc tggcggggat cggcaactgg ttcgtgcgtc tgcaggcgaa acggcacggg
     6661 gccgggctgg cggtctccga gatggtctcc agcttcgccc tccattaccg caacgagcgc
     6721 acgctgcgcg acctgatgcg gatccatccc gacgagcacc cggtctccct gcagctgttc
     6781 ggccacgacg ccgaggtgat gcgctccggg gccgagatcg cggccggggc cggggccgac
     6841 ctgatcgaca tcaacatggg ctgcccggtg ccaaaggtgc gcaagaccgg cgccggcgcc
     6901 cagctcctgc gcgaccccga gctggccctg gcgctggcct gcgccgccaa ggagggcagc
     6961 ggcctgccgg tgaccgtgaa gctgcgctcg gggatcgagg ccggcgaccg ctccggcttc
     7021 gagctggcgg tccggctcgc cgaggaggcc ggggtggcgg cgatcgcctt ccacccgcgg
     7081 gcggcgacca agggccacaa gggccagccc gactatgcgc tgacccgtga gctggtcgaa
     7141 cgggtggagg taccggtgat cgtctccggc gggctgagca gcgcccgggc ggcccgcgcc
     7201 gcctacgagg agtcgggcgc cgacgcggtg atgatcgcgc gcggctcgct cggcaacccc
     7261 tgggtgttcg aggaactgac cggtcagcgg atcgagccgc cgagccgcga cgaaatcgtc
     7321 gccgagctgc tctgggtgat cgaccgcgcc gaggagcacc tggggaccga acgggccgag
     7381 cgctacctgc gtaagttcta cccctggtac agcgaacggc tggacgctcc gccggccctg
     7441 gtggacgagc tccaccgcag cgccgacctc gatcgcgcaa gaaatttgat ccgatcgctg
     7501 gggacgcctg ccggcctaac cagtactgcc tgatagcggg ttttttggcc tcgctataat
     7561 cccgcgctct ttagcccagg tgcggcgaat ggcgacgtcc cccgcggggc gtttttttcg
     7621 ctcagggagc acccttccgg gcttccccaa aacccaaagc gaggtagtgc aagtgagtcg
     7681 ccaggcggta gtcacccccg agggtctcga aaaactgaaa gccgagatcg aggagctttc
     7741 aacggtcaaa cgccgcgagg tcgcggagcg gatcaaggag gcccgggaat tcggcgacat
     7801 ctccgagaac gccgaatacg acgacgccaa gaacgaacag gcgctgttgg agaaccgcat
     7861 cgcccagctc gaagagcgtt tgcgccgtgc cacggtgatc gacgagaagg cgctcagcac
     7921 cgacgaggtc ggcttcggct cgatcgtgca cgtgaaagac cagaaaaccg gcgactcgca
     7981 gaaattccag atcgtcggct cgaccgaggc gaacccgctc gagcacaaac tctcgaacga
     8041 gtcgccgatc ggcaaagcgc tggtcgggaa aaagcggaac gacgtggtca ccgtcgaggt
     8101 gccgcgcggg ccgaagaaga aattcaaaat cacgaaaatc gagaaggctt gaccgaggag
     8161 ctctccgacg tcctcaggga ccgtcgcgag aagctggggc ggatccgcgc ggccgggatc
     8221 gacccgttcc cgcacgagtt ctcccaacgc gaggacatcg ccgaggtgcg cgccgcccac
     8281 gagggtctgg cgccgggaat cgagaccgac tcacgccacc gggtcgccgg gcgcctggtg
     8341 gcccgccgcg gtcacggcaa ggcctgtttc ctcgacctcc gcgacggcag cggtcaggtc
     8401 cagctgcacg cgcgcgagga cctgctcggc cccgaggcct acgagctgct cgtcgatctg
     8461 gacctcggcg acgtgatcgg ggtcgagggg acggcgatgg ccacccggcg cggcggcgag
     8521 ctctcgctcg cgatcgacca ctggcgcctg ctctcgaaga gtctgcggcc gccgcccgac
     8581 aagttccacg ggctcggcga caccgagacg cggttgcgcc gccgtgagct cgacctgatc
     8641 gccaacgagg agagccggcg ggtcttccag gttcgggcac ggacgatcac cgagatccgc
     8701 cgctggctcg acgaacacgg cttcgtcgag gtcgagaccc cggtcctgca gccgctctac
     8761 ggcggcgcct tggcgcggcc gttcgtcacc caccacaacc agctcgaccg cgacttctac
     8821 ctgcggatcg ccaccgagct ctacctcaag cgtctcgtcg tcggcggcat cgatcgtgtc
     8881 tacgagctcg gcaaggactt ccgcaacgag ggcgtctcgc acaagcacaa ccccgagttc
     8941 acgatgctcg agtggta
//
LOCUS       DASRIG010000458         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_453523, whole genome shotgun sequence.
ACCESSION   DASRIG010000458 DASRIG010000000
VERSION     DASRIG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453523"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFB52_12245"
     CDS             <1..577
                     /locus_tag="VFB52_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07150.1"
                     /translation="TGMVATAVIFASALPAMFGTFVKHRFQRTYDAILAAPVDVEELV
                     TAEMLWIGIRAGFYGCFPLLVSFAFGLDPTPGMLLVPLFCFITALGFAAFGIAVAATV
                     AKIDQFNYVTTLVITPLFLVAGTFFPIDQLPEGFQVAAQFNPLYQLVELVRGAAFGFE
                     AVDLIRVAVLAGFSFVMWRVAVHQMAKRLID"
     gene            complement(590..2749)
                     /locus_tag="VFB52_12250"
     CDS             complement(590..2749)
                     /locus_tag="VFB52_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO07151.1"
                     /translation="MRSLTRWVLAHKRTVVAFWIVATIAGAAAAGPASDALEAEFSVP
                     DKEGWETNQVIAERYQGTGGDTAPLVPVVTLPEGESVDSPGVKGELAQIDTRLAEALP
                     AARIASYASTGDDTFVSDDGRTTFAVIYPQPDPNSAFGENPAAEKAASGALEGATVAG
                     QPVHLTGFDALAEDSGADSEGPGVLLEAVLGGLGALVVLTFVFASFLAIVPILMAIVS
                     ILTSFLLLLGLTEFATISPIVQFLIALLGLGVSIDYSLLVVSRWREERSHGRSEDEAV
                     QVAMETAGRAVVFSGITVAIGLFALIALPLPFLRSMGYGGLLIPLVSTLVAITLLPVV
                     MAKLGPRLDWPHRRTDDKASRAWTRWAEAVSRRRWLAAGAGMAVVLALVLAATDLQLG
                     TSDANTMAQSGDAKEGLTALEDAGIGEGSLLPHEILIEGGTDPDAVAADVGEVDGIQG
                     AVAPDNGDWRRSGTALVEAIPIPDSGSDEGEATLEGVREAAHAAGPDVRVGGQPAAND
                     DFIDAVYGSFPLMIALITVTTFILLARAFRSLLLPLKAILLNILSVAAAWGVLVLVWQ
                     HGYGSELIWGIEATGSIPSWMPLMIFAFLFGLSMDYEVFILARMREEYDRTGRTDYAV
                     VQGIGRTGRLVTSAALILFLAFVSMASSPGTDLKMFATGLGAGILLDATVIRALIVPA
                     VISLMGRWNWWLPSWPAKLLRVEPSVLTPPEPRRAEA"
     gene            complement(2762..2995)
                     /locus_tag="VFB52_12255"
     CDS             complement(2762..2995)
                     /locus_tag="VFB52_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07152.1"
                     /translation="MFGDQIEQVSVTATEARDHVAELEAERALAEVTGVAEIHSYISD
                     LEEELGIWRELYVITAVTEIATLRGELSGVLVG"
     gene            3154..>3549
                     /locus_tag="VFB52_12260"
     CDS             3154..>3549
                     /locus_tag="VFB52_12260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZO07153.1"
                     /translation="MITQTEPRTLLYTTLESPVGELLLVGDGERLRGLHFQEGLRRVR
                     VQDSWRRAEEPFAEARDQLGDYFAGERAEFDLPAAAEGTPFQRRVWDALAEIPYGETL
                     SYGELAGRIGRPSASRAVGMANGRNPISIV"
BASE COUNT          543 a         1242 c         1234 g          530 t
ORIGIN      
        1 caccggcatg gtcgccacag cggtgatttt cgcgagcgcg ctgccggcga tgttcgggac
       61 cttcgtcaag caccgattcc agcgcaccta cgacgcgatc ctcgccgcgc ccgtcgacgt
      121 cgaggagctg gtcaccgcag agatgctctg gatcgggatc cgtgccggct tctacggctg
      181 cttcccgctg ctggtctcgt tcgccttcgg gctcgatccg acgcccggca tgctcctcgt
      241 accgctcttc tgcttcatca cggcgctcgg ctttgccgcc ttcggaatcg cggtcgccgc
      301 caccgtggcg aagatcgacc agttcaacta cgtgaccacg ctggtgatca cgcccctctt
      361 cctggtcgcc gggaccttct tcccgatcga ccagctgccc gagggctttc aggtcgcggc
      421 ccagttcaac ccgctctacc agctcgtgga gctcgttcgc ggcgccgcct tcgggttcga
      481 ggcggtcgat ctgatccggg tcgcggtcct ggcaggcttc tcgttcgtca tgtggcgcgt
      541 cgccgtgcac cagatggcga agcggctgat cgactgatcg gcgcccggct caggcctcgg
      601 cccgccgtgg ctccggcggc gtgagcaccg agggctcgac ccgcagcagc ttcgccggcc
      661 agctcggcag ccaccagttc cagcgcccca tcagcgagat caccgcgggc acgatcagtg
      721 cccggatcac ggtcgcgtcg agcaggatgc cggcgccgag cccggtcgcg aacatcttca
      781 ggtcggtgcc gggactggac gccatcgaaa cgaacgcgag gaagaggatc agcgcggcgc
      841 tggtcaccag ccgcccggtg cgcccgatgc cctggaccac cgcgtagtcg gtgcggcccg
      901 tgcggtcgta ctcctcccgc atccgggcga ggatgaagac ctcgtagtcc atcgagagcc
      961 cgaacaggaa ggcgaagatc atcagcggca tccacgacgg gatcgagccg gtggcctcga
     1021 tgccccagat cagctctgag ccgtacccgt gctgccagac caggaccagg acgccccagg
     1081 ccgcggccac gctcaggatg ttgagcagga tcgccttcag gggcagcagg agggagcgga
     1141 aggcgcgggc gagcaggatg aaggtcgtga cggtgatcag cgcgatcatc agcgggaagc
     1201 tgccgtagac cgcatctatg aagtcgtcgt tggcggccgg ctgcccgccg acacggacgt
     1261 cggggcctgc cgcgtgtgcg gcctcgcgga ccccctcgag cgtcgcctcg ccctcgtcgg
     1321 agccgctgtc ggggatcggg atcgcctcga ccagcgcggt cccgctgcga cgccagtccc
     1381 cgttgtccgg ggcgacggcg ccctggatac cgtccacctc gcccacgtcg gcggcgaccg
     1441 cgtcggggtc ggtgccgccc tcgatcagga tttcgtgcgg gagaagggag ccctcgccga
     1501 tgcccgcgtc ctcgagcgca gtgaggccct ccttcgcatc gccggactgg gccatcgtat
     1561 tggcgtccga ggtcccgagc tgcaggtcgg tggccgcgag cacgagcgcc agcacgacgg
     1621 ccatcccggc gccggcggcc agccagcgcc gtcgcgagac ggcctcggcc caccgcgtcc
     1681 aggcccggct cgccttgtcg tcggtgcgcc ggtggggcca gtccaggcgc gggcccagct
     1741 ttgccatcac caccggaagc agggtgatcg caaccagggt cgagaccagc gggatcagca
     1801 ggccgccgta gcccatcgag cgcaggaagg gcagcggcag cgcgatcagc gcgaagaggc
     1861 cgatcgcgac cgtgatcccg ctgaagacga cggccctccc ggcggtctcc atcgccactt
     1921 ggacggcttc gtcctcgctg cgaccgtgcg agcgctcctc gcgccagcgt gagacgacga
     1981 gcagtgagta gtcgatcgac accccgaggc cgagcagcgc gatcaggaac tgaacgatcg
     2041 gtgagatggt cgcaaactcg gtgagtccca gcaggagcag gaagctggtc aggatcgaga
     2101 cgatggccat gaggatcggc acgatcgcga ggaacgacgc gaagacgaac gtaagaacga
     2161 ccagcgcgcc gagcccgccg agcacggcct cgagcaggac gcccgggccc tcggaatcgg
     2221 cgccgctgtc ctcagccagc gcgtcgaagc ccgtcagatg cacgggctga ccggcgaccg
     2281 ttgcgccctc gagcgcgccg ctcgccgcct tctcggccgc ggggttctcg ccgaaggccg
     2341 agttcgggtc gggttgcgga tagatgacgg cgaaggtcgt ccggccgtcg tcggatacga
     2401 acgtgtcgtc tccggtcgaa gcgtaggagg cgatccgggc cgccggcagc gcctcggcga
     2461 ggcgtgtgtc gatctgcgcc agctcgccct taacgcccgg ggagtcgacg gactcaccct
     2521 cggggagagt cacgacggga accaggggag cggtgtcccc gccggtgccc tggtaacgct
     2581 cggcgatcac ctggttggtc tcccagccct ccttgtcggg aaccgagaac tcggcctcga
     2641 gcgcgtcgga cgccggtccc gcggccgccg ccccggcgat ggtggcgacg atccagaagg
     2701 cgacgacggt gcgcttgtgg gcgaggaccc agcgggtgag cgatcgcacg agctccggcc
     2761 cctagccgac caggacgccg gacagctcgc cgcggagcgt cgcgatctcg gtgacggcag
     2821 tgatcacgta gagctcgcgc cagatcccga gctcctcctc gaggtcgctg atgtaggagt
     2881 ggatctccgc caccccggtg acctcggcaa gcgctcgctc cgcctccagt tccgccacgt
     2941 ggtcccgggc ctcggtcgcc gtcaccgata cctgctcgat ctggtctccg aacatgccta
     3001 gaggcttcca tcgtggcctg ccggagaaaa tgcggctaac tggcgtgctg gggttcggtt
     3061 tgccgccccc cagcggggct gagcaatgtc cggagcacct cacgccgcag acctcgcgtc
     3121 cgttggatcc tgtgagcgac aacggaggac gcgatgatca cccagaccga accgaggacc
     3181 ctgctctaca cgactctcga gagcccggtc ggcgagctgc tgctggtcgg cgacggcgag
     3241 cgcctgcgcg ggcttcactt ccaggagggc cttcgccgcg tgcgggtcca ggattcgtgg
     3301 cggcgcgccg aggagccgtt cgccgaggcc cgggaccagc tcggcgacta cttcgccggc
     3361 gagcgggccg agttcgatct gccggccgcc gccgagggaa cgccgtttca gcggcgggta
     3421 tgggacgcgc tggccgagat tccctacggc gagaccctca gctacggcga gctcgccggg
     3481 cggatcggca ggcccagtgc ctcgcgcgcc gtggggatgg cgaacgggcg caatccgatc
     3541 tcgatcgtg
//
LOCUS       DASRIG010000459        11784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2029461, whole genome shotgun sequence.
ACCESSION   DASRIG010000459 DASRIG010000000
VERSION     DASRIG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029461"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFB52_12265"
     CDS             <1..185
                     /locus_tag="VFB52_12265"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZO07154.1"
                     /translation="RQFRSPELALAAYNAGPGAVEPCNCIPPYPETQAYVTRILGLLG
                     GAGALLMPSFEVRLIG"
     gene            230..1276
                     /locus_tag="VFB52_12270"
     CDS             230..1276
                     /locus_tag="VFB52_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07155.1"
                     /translation="MNVATDRSLISLIGDAFSLYARYPLLFVTLASLVIVPYQLLVLL
                     ATGAGPFEIESVDVGTGFLLETVDWALVVPLISALHVHAVADVKEGETPRLGSVSRRS
                     LAALPVVAAASVMSGLGILLGFVALIIPGIYLLLRWSMVAQAAAIERNGWGNALRRSS
                     ELMDGFYWRVLGFFLLLGLLAALPFIPTFFLFDEEGTTVASFLIGVFLEVVTWSFVAL
                     GTALLYFDLRSRGQRSLAAMPPPIPPGTPAAVTPQQPSQPASEHDLDPRVYPDEERPV
                     GWYVDPHAPYRMRYWGGEQQGWSPKTARTPGKTRRAWDRRWDTPKPPPPPPPPPPPSP
                     PPPPPPPPPPPPSA"
     gene            complement(1273..2856)
                     /locus_tag="VFB52_12275"
     CDS             complement(1273..2856)
                     /locus_tag="VFB52_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO07156.1"
                     /translation="MANPTSRVLSSSQFETLAEHGEERSAEVGEVLFRVGDRTYPFIA
                     IIEGEASVRDAAGREVVRHGTGGFLGEMNLLSGQTVFLTAVVTEPMRYIAVDREDLRR
                     LLFDDASLADLLLPAFIQRREALQQIQGLGLEIIGSPLQEETRRLVEFAKGLRLPHTP
                     RDPAADAEAATLIEALREEELPLVLLPGGAELRRPSNGELSRALGIGLELAPREEVDL
                     LVVGGGPAGLGAAVYGASEGLDTLVVESKVLGGQAGTSRRLENYLGFPAGISGSELIS
                     RAITQARKFSARTATPYRALSLQPGDERHTVQLEDGVEVAARAVLLSTGAEYRKLPVA
                     DLHEYEGFSVFYAAGPPEAQLCGAQRVGVVGGGNSAAQAAIWLARGGALVTLLHRRAD
                     LRETMSNYLIGDLDRYGVDVRDRSEISELHGADGRLEGVTLTDGTELPFSFLFLFLGA
                     SPCTDWLGDVVARDEKGFILTGEEAGANGLLETSVPGVYAGGDVRSGSIKRCATAVGE
                     GATVVRFVHERFAAAAVPS"
     gene            2945..3334
                     /locus_tag="VFB52_12280"
     CDS             2945..3334
                     /locus_tag="VFB52_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO07157.1"
                     /translation="MPTPYTAMRLTNVKDSAKEFGVGEDMEVRFAKDELDAERTGVSH
                     HRIKPGKRQPFAHQHDEAEEIYVVLGGSGRFKLDDEVIDVEPLDAIRVAPGVIRCFEA
                     GPGGIEVLVVGARHDGDGRIIPGWWSD"
     gene            complement(3336..4709)
                     /locus_tag="VFB52_12285"
     CDS             complement(3336..4709)
                     /locus_tag="VFB52_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZO07158.1"
                     /translation="MSSEIERKFVLDGLPSRLAERDGVAISQGYLTNGEGPEIRLRRA
                     GEATLLTVKTGDGEVREEVEVSLAPELFKTLWPLTAGRRIAKTRLREPLGEELVAEVD
                     VYGEGLSGLVVAEVEFDSEQRSREFEPPPWLGREVTGDPRYANRELAMHGTAPIEQTE
                     KKDEPSRSFRLKRKEDAGDGLRRIVRGRTAQAIESLEGIEEDPVAAIHSARKDIKKIR
                     SVLRLLRDDLGKDLYRTENRRYRDAAAGLSGSRDAAVKVETLTALEDHFGREIPSASA
                     WGWRRQLERERLEAAEADPEAVAAAIAALRAGGEAVAEWPLDDGSWDLVGPGLERSYR
                     RGRAAMAEVADDPDPELVHAWRKRGKDLWYQLRLVKRAWPSLLGETVDRTHELTDLLG
                     DHHDLSVLATDLASREIGSKARMTELIGQRQGELLGEALALGERLYAEKPKAFGKRVH
                     AYWSAWR"
     gene            4757..5230
                     /locus_tag="VFB52_12290"
     CDS             4757..5230
                     /locus_tag="VFB52_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZO07159.1"
                     /translation="MPAVEVNGVEGMQALVGQEVGPSEWRTVTQEDIDTFAELSGDHQ
                     WIHVDVERAKTESPFGTTIAHGNLTLSLVDGFRQELINATGFALGVNYGWNKIRFPAP
                     VPSGTRVRGRAELVSMTEIGGGWWEYITRFTLEVEGNEKPCFVGESVVRVMAPAE"
     gene            complement(5258..6838)
                     /locus_tag="VFB52_12295"
     CDS             complement(5258..6838)
                     /locus_tag="VFB52_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZO07160.1"
                     /translation="MAFGVLASALTAFAIVGGSKAESNRYPWIVQIRVEGDLCAGSLI
                     RPRWIVTTAHCVTDEKTRKPVEGSRLTARVGNNELDKGGELRTIDWAIPYPGYNGSQS
                     IDLALARLDQEVSTTDPVRLVGLARGTLIGPGDRPSIAGWGMRGRRTLLPPKASPSLR
                     QAQVRLAPSVICERERDAEWPFEPATQLCTTAGSGEVCHGDSGGPLFVDRGDGTESLI
                     GVTQGGNRYCAPGHGNRFARLTGGPMREWLDAQLYAIGRPLSRCRDQVIRHGFEMHTI
                     KRLRSNTGCSTGGAIAIDVAERDECVEETSGGTNFCRSGDFICGTEIVNGPAGVAFVS
                     ACQDGTRRITFRQGSASFSRGAPYRLRFDSRGFPAAVGRFDIAGGDRSISDMIDAFGQ
                     PDSTGGDATACTITWSALGLTAHAVDYGASEACDPTQGLINAITMRSPRFLTDRGAIV
                     GISRGELQERHPEATTRGMPGDAYFDGEPAFGQGDLYVLHTFASPIGLGGRLATLTAL
                     VSDDAVVGFQVTPLLGGD"
     gene            complement(7008..7472)
                     /locus_tag="VFB52_12300"
     CDS             complement(7008..7472)
                     /locus_tag="VFB52_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZO07161.1"
                     /translation="MKKALIVALALIAASLALVACGGSDDSSDTTAAETQAESTESGG
                     EAAGGGEAEGGTAGSAAVLDFETPEGTDLAFTTTEADATAGKVTVNFTNVQAIPHDVD
                     IEDEAGESVGKTDLISEDSASTEVDLKPGTYTFFCSVPGHREAGMEGTLNVK"
     gene            complement(7558..8382)
                     /locus_tag="VFB52_12305"
     CDS             complement(7558..8382)
                     /locus_tag="VFB52_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO07162.1"
                     /translation="MSTQTLSPPELNTMRIEIDGEIGTLTLDRPDAFNAMSPELIGEM
                     IVAFGWLADRAPLRALILTGAGKAFCAGGDVTWFRKGVEDDEYDLPSDVRRGAEALHQ
                     AIIDLRRIPYPVIGAINGPAAGAGFSLALACDVRIASEHAFFAPAYGRIGASPDGGMT
                     YFLPRVVGPSRALELLLDDPNMPAADALEEGIVSEIVPHDELTDRARAKAEQWAAMAP
                     HYVKMAKRLVGVSIENSINDHLQLERHGIADSMATEDLRAGVTAFFGGEKPVFTGK"
     gene            8457..10358
                     /gene="uvrC"
                     /locus_tag="VFB52_12310"
     CDS             8457..10358
                     /gene="uvrC"
                     /locus_tag="VFB52_12310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZO07163.1"
                     /translation="MAKTQPDKQLSRAERIAEQRRALPDSPGVYLFYGRDDELLYVGK
                     ARSIRKRVASHFSGGDTRLTRRIERIDTLVTANEAEALLAEQSFIKRHRPRFNIRLRD
                     DKSYPYVAVSLDEDYPRVYFTREKHRSGRAYFGPFSSAKRVRETLDLLGKLFQFRTCE
                     GVEPGRRSGSPCLDYYIKRCGAPCVGHVSKEEYRLNIEAIVDFLSGRYRQVEAEEQAK
                     MEDAAGKEEFERAAIHRDRLKAIRSLFERQRVAGGSVGTADLIGVAVEGEDANAQVFQ
                     VRDGILAERQSFYLENQAERDPAEVAEEFIGQYYAASPSMPRTIIVGPYLRGRTEVLS
                     QALSERRGSPVEVRAAERGDKRKLRELAERNAKLALDQDRLRREHRRARRVESLSSLQ
                     EALGMEELPVRIEGFDISNIGGEHTVASMVVFEGGATKKADYRRFKVRGDLKGEGGAN
                     GGPDDFASMEEVLGRRMNRYLEQADLSPHDGKRDASFASLPSLILIDGGKGQLAAGMR
                     ALAPFAERGVTVVSLAKREEEVFVPGRSQPLDLHANSEGSKLLQRVRDEAHRFAIDHH
                     RGRRDQAMTVSVLDELKGVGPARKRALLQHFGSPERFLAATREELEAVPGLPGKVGRE
                     IHEQLNRIG"
     gene            10385..11260
                     /gene="rapZ"
                     /locus_tag="VFB52_12315"
     CDS             10385..11260
                     /gene="rapZ"
                     /locus_tag="VFB52_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZO07164.1"
                     /translation="MSDGKVTLVVITGHSGAGKSEAIAAFEDGGYFCVDNLPPRMIEG
                     LGELFRHKGSGVERAAIVSDVRGGEYFDDLLAVLDDLDADGLKPTVLFLEADEETLLD
                     RYKETRRRHPLAPEGRIVEGIRAEHELLAPLRERADMVMDTSDLTGAELRRRIAEEML
                     DGEEGGKLALTLLTFGFKNGPPRDADLTLDVRFLPNPHYVDELRPLTGMDEVVRTYVE
                     SGTQAGEFYGRLMPLLQFLVPAYVAEGKSHLTIAVGCTGGRHRSVTVADRIRRDLADI
                     EGVIVKVKHRDLPAE"
     gene            11320..>11784
                     /locus_tag="VFB52_12320"
     CDS             11320..>11784
                     /locus_tag="VFB52_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO07165.1"
                     /translation="MPSPVISISGLRMSYGEVEAVRGIDLEVMPGEIFAFLGPNGAGK
                     TTTVEILEGYRKRSGGAVSVLGEDPQRAGREWRERIGIVLQSGRLDPYLTVRESLGLY
                     AGYYRNPRPVEEVIALVGLEEKADTRARKLSGGQQRRLDVGMALVGDPELLFL"
BASE COUNT         1719 a         4113 c         4090 g         1862 t
ORIGIN      
        1 tccgccagtt ccgctcgccc gaactcgccc tcgctgctta caacgccggc cctggcgcgg
       61 tcgaaccatg caactgcatc ccgccatatc ccgagaccca ggcctacgtg acccgcatcc
      121 tcggcctgct cggcggcgcc ggcgctctgc tgatgccgag cttcgaggtg cgcctgattg
      181 gctgagggtc gcctagtatt ccgacgctga tgcactggcg ggggctcgca tgaacgtcgc
      241 aaccgaccga tccttgatct ctctgatcgg ggacgcgttc tcgctctacg cgcgttaccc
      301 gctgctcttc gtcaccttgg cctcgctggt gatcgtgccc taccagttgc tcgttctcct
      361 ggcgacgggt gccgggccgt tcgagatcga atcggtcgac gtggggaccg ggttcttgct
      421 cgaaacggtc gactgggcgc tggtcgtgcc gctgatttcg gccttgcacg tgcacgccgt
      481 tgcagacgtg aaggagggtg agacgccgcg gctggggtcg gtctcgcgcc gcagcctggc
      541 ggcgcttcca gtcgtagcgg ctgcctccgt gatgtcgggg ctcggcatcc tgctcggctt
      601 tgtcgccctg atcatccccg ggatctacct gctgctgcgc tggtcgatgg tcgcccaggc
      661 cgcggcgatc gagcgcaacg gctggggcaa tgcgctgcgc cgcagcagcg agctgatgga
      721 cggcttctac tggcgggtgc tgggcttctt tctcctcctc ggcctgctcg cggcgctgcc
      781 gttcatcccc acgttcttcc tcttcgacga agaggggacg acggtggcct cgtttctgat
      841 cggcgtcttt ctcgaagtgg tcacctggtc gttcgtcgcg ctcggcactg cgctcctcta
      901 cttcgacctg cgcagtcgcg ggcagcgctc gctggcggcg atgccccctc cgattccccc
      961 cggaaccccc gccgcggtta ctccccagca accgtcccag cccgcctccg agcacgacct
     1021 cgacccgcgg gtctacccgg atgaggagcg tccggtcggc tggtacgtcg acccccacgc
     1081 gccctaccgg atgcgctact ggggcggtga gcagcaaggt tggtcgccga agacggcccg
     1141 taccccgggc aagacgaggc gcgcctggga tcgccgctgg gatacgccca aaccgccgcc
     1201 gccaccacct ccacctccgc cgcccagccc cccgcctcct cccccgccgc caccaccgcc
     1261 ccctccgtcc gcttaggacg ggacagcagc ggccgcgaag cgctcgtgca cgaagcggac
     1321 gacggtcgca ccctcgccaa ccgcggtcgc gcagcgcttg atcgagcccg agcgcacgtc
     1381 gccgccggcg tagacgccgg gcacgctcgt ctccagcagg ccgttggcgc cggcttcctc
     1441 gccggtcagg atgaagccct tctcgtcacg ggcgacgacg tcgccgagcc agtcggtgca
     1501 gggcgaggcg ccgaggaaga ggaagaggaa ggagaagggg agctcggtcc cgtcggtcag
     1561 cgtcacgccc tcgagccggc catcggcgcc atgcagctcg ctgatttcgc tgcggtcgcg
     1621 gacgtcgact ccgtagcggt cgaggtcgcc gatcaggtag ttcgacatcg tctcccgcag
     1681 gtcggcgcgg cggtgtagga gggtgacgag ggcgccgccg cgggccagcc agatcgccgc
     1741 ctgcgcggcc gagttgccgc cgccgacgac gccgacgcgc tgggcgccgc agagctgcgc
     1801 ctcgggtggt ccggcggcgt agaagacgct gaagccctcg tactcgtgca ggtcggcgac
     1861 cggcagtttg cggtattcgg cgccggtcga gagcagcacc gcgcgggccg cgacttccac
     1921 cccgtcctcg agctgaaccg tgtggcgttc gtcgccgggc tgtagcgaga gcgcccggta
     1981 gggcgtcgcg gtgcgggcgc tgaacttgcg cgcctgggtg atcgcgcggc tgatcagttc
     2041 gctgccgctg atcccggccg ggaagccgag gtagttctcg agccgccgcg aggtgcccgc
     2101 ctgtccgccc agcactttgc tctcgacgac cagcgtgtcg aggccctcgg aggcgccgta
     2161 gacggcggcg ccgagcccgg ccggcccacc accgacgacg agcaggtcga cctcctcgcg
     2221 cggcgccagc tccagcccga tcccgagcgc ccgcgagagc tccccgttcg acggccgccg
     2281 cagctcggcc ccgcccggca gcagcaccag cggcagctcc tcctcgcgca gagcctcgat
     2341 cagcgtcgcc gcctcggcgt cggcggcggg gtcgcgcggg gtgtggggga ggcgcagccc
     2401 tttcgcgaac tcgaccagcc gccgggtctc ctcctgcagc ggcgagccga tgatctccag
     2461 cccgagcccc tggatctgct gcagcgcttc gcggcgctgg atgaaggcgg gcagcaggag
     2521 gtcggcgagc gaggcgtcgt cgaagagcag ccggcgcagg tcttcgcggt cgacggcgat
     2581 gtagcgcatc ggctcggtga ccacggcggt gaggaagacg gtctgcccgg agagcaggtt
     2641 catctcgccg aggaagccgc cggtgccgtg gcggacgacc tcgcgcccgg cggcgtcgcg
     2701 gaccgaagcc tcgccctcga tgatcgcgat gaacgggtag gtccggtcgc cgacccggaa
     2761 gaggacctcg ccgacctccg cgctgcgctc ctcgccgtgc tcggcgagcg tctcgaactg
     2821 cgagctggag aggacccgag aggttgggtt ggccacgcgc caaggctacc ccgtgcggca
     2881 agccggcttt tcggtctggg cgccgctggg tagggggctg agaggaccgg aaggaggagg
     2941 accaatgcca acgccctaca ccgcgatgag actcaccaac gtgaaggact ccgccaagga
     3001 gttcggagtc ggcgaggaca tggaggttcg cttcgccaag gatgagctcg acgctgagcg
     3061 caccggggtc agccaccacc ggatcaagcc gggcaagcgc cagccgttcg cgcaccagca
     3121 cgacgaggcg gaggagatct acgtcgtgct cggcggctcg ggccggttca agctcgacga
     3181 tgaggtgatc gacgtggaac cgttggacgc gatccgcgtc gcccccgggg ttatccgctg
     3241 cttcgaggcc gggcccggcg ggatcgaggt gctcgtggtc ggcgcccgcc acgacggcga
     3301 cggccggatc atccccggct ggtggagcga ctgagctagc gccaggccga ccagtaggcg
     3361 tgcacgcgct tgccgaacgc cttcggcttc tccgcgtaga gccgctcgcc gagcgccagc
     3421 gcctctccca gcagttcgcc ctgccgctgt ccgatcagct cggtcatccg cgccttgctg
     3481 ccgatctcgc ggctcgcgag gtcggtggcg agcacgctga ggtcgtggtg gtcgccgagc
     3541 aggtcggtga gctcgtgggt gcggtcgacg gtctcgccga gcagcgaggg ccaggcgcgc
     3601 ttgaccaggc gcagctggta ccagaggtcc ttgccgcgct tgcgccaggc gtggacgagc
     3661 tctgggtcgg gatcgtcggc gacttccgcc atcgccgcac ggccgcgccg gtagctgcgc
     3721 tcgaggccgg ggccgaccag gtcccatgac ccgtcgtcga gcggccactc ggcgacggcc
     3781 tcgccgccgg ccctgagcgc cgcgatcgcc gccgcgaccg cctcggggtc agcctcggcc
     3841 gcttccaatc gctcgcgctc cagctggcgc cgccagcccc aggcggaggc gctcggaatc
     3901 tcgcggccga aatggtcctc cagcgcggtc agggtctcga ccttgaccgc ggcgtcgcgg
     3961 ctgccggaga ggccggcggc ggcgtcgcgg taacgccggt tttcggtccg gtagagatcc
     4021 ttgcccaggt cgtcgcgcag cagccgcagg accgagcgga tcttcttgat gtctttgcgc
     4081 gccgagtgga tcgcggcgac cggatcctcc tcgatgccct cgagcgactc gatcgcctgc
     4141 gcggtgcggc cgcggacgat ccggcgcaga ccatcgcctg cgtcctcctt gcgcttcagc
     4201 cggaagctgc gcgagggttc gtccttcttc tcggtctgct cgatcggcgc ggtgccgtgc
     4261 atcgccagct cccggttcgc gtagcgggga tcaccagtta cctccctacc cagccagggc
     4321 ggtggctcga actcgcggct gcgctgctcg gagtcgaact cgacctcggc gacgaccagc
     4381 ccgctgaggc cctcgccgta gacgtcgacc tcggcgacca gctcctcgcc gagcggctcg
     4441 cgcaggcgcg tcttcgcgat ccgccggccc gcggtcagtg gccacagcgt cttgaacagc
     4501 tccggcgcca gcgacacctc gacctcctcg cggacttcgc cgtcgccagt cttcacggtc
     4561 agcagcgtcg cttcaccggc ccggcgtaag cggatctccg ggccctcgcc gttcgtgagg
     4621 tagccctggg aaatcgcgac cccgtcgcgc tcggcaagcc gggaggggag gccgtcgagc
     4681 acgaacttgc gctcaatctc gctgctcatg ccctcaatct agaggtcctc gggcgcttcg
     4741 cataggatct gcgccgatgc cagccgtcga ggtaaacggg gttgagggga tgcaggcgct
     4801 ggtcggccag gaggtcggcc cgagcgagtg gcgcactgtc acccaagagg acatcgacac
     4861 cttcgccgag ctctcgggcg accaccagtg gatccacgtc gacgtcgagc gggcgaagac
     4921 cgagagcccc ttcggcacca cgatcgccca cggcaacctg acgctctccc tcgtcgacgg
     4981 cttccgccag gaactgatca acgccaccgg cttcgccctc ggcgtcaact acggctggaa
     5041 caagatccgc ttcccggccc cggtcccctc cggcacccgc gtccgcggcc gcgccgagct
     5101 ggtctcgatg accgagatcg gcggcggctg gtgggagtac atcacccgct tcacgctcga
     5161 ggtcgagggc aacgaaaagc cctgcttcgt cggcgaaagc gtcgtccgcg tgatggcgcc
     5221 tgccgagtag cttcgcggca agctgcagca cctctggcta gtcgcctcca agtaggggcg
     5281 tgacctggaa tccgaccacc gcgtcgtcgg agacaagggc ggtgagggtc gccaggcggc
     5341 cgccgagccc gatcgggctg gcgaaggtgt gaagaacgta gaggtcaccc tgcccaaacg
     5401 cgggttcgcc atcgaaatac gcgtcgccgg gcattcctcg ggtcgtcgct tccggatggc
     5461 gctcctgcag ttccccgcgg ctgataccga cgatcgcgcc gcggtcagtc aggaatcgag
     5521 gcgagcgcat cgtgatcgcg ttgatcaagc cttgcgtcgg atcacatgct tccgaggctc
     5581 cgtagtcgac tgcgtgtgcc gtcaggccta gcgcgctcca ggtaatggtg caggcagtgg
     5641 cgtcgccccc ggtgctgtct ggttggccga aggcgtcgat catgtcgctg atcgaccgat
     5701 ctccgcctgc gatatcgaat cgaccgactg cggcagggaa tccccgggag tcgaagcgaa
     5761 ggcggtatgg cgcgccgcgg gagaagcttg ccgagccctg gcgaaacgtg atcctgcgcg
     5821 ttccgtcctg gcaggcggag acgaacgcca cgccggcggg gccgttgacg atctcggtgc
     5881 cgcaaatgaa gtctcccgat ctgcagaagt tggtgccgcc gctggtctcc tcgacgcatt
     5941 cgtccctttc ggcgacatcg atcgcgatcg ccccgccggt cgagcaccca gtgttgctgc
     6001 gcaggcgctt gatcgtgtgc atctcgaagc catggcggat gacctgatct cggcatcggc
     6061 tcaagggtcg tccgatcgcg tagagctggg cgtcgagcca ctccctcatc gggcccccgg
     6121 tgaggcgggc gaagcgattg ccatgcccgg gcgcgcagta tcggttgccg ccctgcgtca
     6181 ctccgatcag gctctcggtg ccatcgcctc ggtcgacgaa tagcggtccg ccgctgtcgc
     6241 cgtggcagac ctcgccactg ccggccgtcg tgcagagctg ggttgccggc tcaaagggcc
     6301 attcggcgtc ccgttcacgc tcgcagatga cacttggcgc caatctgact tgggcctggc
     6361 gtagcgaggg agaagctttc ggcgggagca gcgtgcgccg gccgcgcatg ccccatccgg
     6421 caatcgacgg gcggtctccg gggccgatca gcgtgccgcg ggcgagcccg acaaggcgca
     6481 ccgggtcagt ggtcgagacc tcctggtcca ggcgcgcaag cgcgaggtcg atgctctgcg
     6541 acccgttgta gcccggatac gggattgccc agtcgatcgt gcgcagctcg ccgcccttgt
     6601 cgagctcgtt gttgcccacc cgcgcggtga ggcgcgagcc ttcgaccggc tttcgggtct
     6661 tttcatcggt tacgcagtgg gccgtggtca cgatccaccg tgggcgtatc aacgatccgg
     6721 cacagaggtc cccctcgacc ctgatttgga cgatccaggg gtagcggttc gactccgcct
     6781 tgctgccacc gacgatcgca aatgcggtca gcgcggatgc gagcacgccg aacgccacca
     6841 acgctgcgac ccacacgcgc ctccccatgc ggtcgattat gggcgaccgc ggaacgtctc
     6901 aggccgacgc ttgagtacgt cagtggaggc gagggttcga gactcgcgct cccgcctttg
     6961 tacgccctat agcgcgtaca aaggcgggag cgcgaaatcc ctccccgcta tttgacgttc
     7021 agggtgcctt ccatgcccgc ctcgcggtgg ccggggactg agcagaagaa ggtgtaggtg
     7081 ccgggtttga ggtcgacctc ggtcgaggcg gagtcttcgg agatcaggtc ggttttgccg
     7141 acgctttcac ccgcttcgtc ttcgatgtcg acgtcgtggg ggatcgcctg gacgttggtg
     7201 aagttgaccg tgactttgcc cgcggtcgcg tcggcttcgg tggtcgtgaa ggcgaggtcg
     7261 gtgccctcgg gggtttcgaa gtcgaggact gcggcgctgc cggcggtgcc gccttcggct
     7321 tcgcctccgc cggccgcttc gccgccggat tcggtgcttt cggcctgggt ttcagcggcg
     7381 gtggtgtccg agctgtcgtc ggagccgccg caggcgacca gcgccagtga ggcggcgatc
     7441 agcgctaggg cgacgataag ggcctttttc atgtcggtcc tttcggtgcg gtggactggt
     7501 ctgatttggt gatacgtcgg caccggcgag tgggttcacc cggcgcctcc ctggtgctta
     7561 cttacccgtg aacacgggct tctcaccgcc gaagaaggcg gtcactccgg ctcgcaggtc
     7621 ctcggtcgcc atgctgtcgg cgatcccgtg gcgctcgagc tgcaggtggt cgttgatcga
     7681 gttctcgatg ctgaccccga ccaggcgctt ggccatcttc acgtagtgcg gggccatcgc
     7741 cgcccactgc tcggccttgg cgcgggcgcg gtcggtgagc tcgtcgtggg gcacgatctc
     7801 gctgacgatg ccctcctcga gcgcgtcggc cgccggcatg ttggggtcgt cgagcagcag
     7861 ttcgagcgcc cgcgagggac cgaccacgcg cggcaggaag taggtcatgc cgccgtccgg
     7921 cgaggcgccg atgcggccgt aggcgggggc gaagaaggcg tgctcggagg cgatccggac
     7981 gtcgcaggcg agcgccagcg agaacccggc cccggccgcc ggcccgttga tcgcgccgat
     8041 caccggatag gggatccggc gcaggtcgat gatcgcctgg tgcagcgcct cggcgccgcg
     8101 gcgcacgtcg gagggcaggt cgtactcgtc gtcttcgacc cctttgcgga accaggtgac
     8161 gtcgccgccg gcgcagaagg ctttgcccgc cccggtcagg atcagcgccc gcagcggcgc
     8221 ccggtcggca agccagccga aggcgacgat catctcgccg atcagttccg ggctcatcgc
     8281 gttgaaggcg tccggccggt cgagcgtcag ggtcccgatc tccccgtcga tctcgatccg
     8341 catcgtgttc agctccggcg ggctcagcgt ctgcgtactc atctgccttc cgtctccctc
     8401 tccgttctcg tttgccgcaa atttggcgga gtgacgggga gcctaccctt tgcctgatgg
     8461 ccaagaccca gccagacaag cagctctcac gcgccgagcg gatcgccgag cagcgccgcg
     8521 cgctgcccga ttcgcccggc gtctacctct tctacgggcg cgacgacgag ctgctctacg
     8581 tcggcaaggc gcgctcgatc cgcaaacgcg tcgcctcgca cttctccggc ggcgacaccc
     8641 gcctgacccg ccggatcgag cgcatcgaca ccctggtcac cgccaacgag gcggaggccc
     8701 tgctcgccga gcagagcttc atcaagcgcc accggccgcg cttcaacatc cgcctgcgcg
     8761 acgacaagtc ctacccctat gtcgcggtca gcctcgacga ggactacccg cgggtctact
     8821 tcacccgcga gaagcaccgc agcgggcgcg cctacttcgg gcccttctcc agcgccaagc
     8881 gggtgcgcga gacgctcgac ctgctcggca agctgttcca gttccgcacc tgcgaggggg
     8941 tggagcccgg tcgccgctcc ggcagcccct gcctcgacta ctacatcaag cgctgtgggg
     9001 cgccctgtgt gggccacgta agcaaggagg aataccggct caacatcgag gcgatcgtcg
     9061 acttcctctc cggccgctac cgccaggtcg aggccgagga gcaggcgaaa atggaggacg
     9121 cggccggcaa ggaggagttc gagcgggcgg cgatccaccg cgaccggctg aaagcgatcc
     9181 gctcgctgtt cgagcgccag cgcgtcgccg gcggctcggt cgggacggcg gacctgatcg
     9241 gggtcgcggt cgagggcgaa gacgccaacg cccaggtctt ccaggtccgc gacgggatcc
     9301 tcgccgagcg ccagagcttc tatctggaga accaggccga acgcgacccg gccgaggtcg
     9361 ccgaggagtt catcggccag tactacgcgg cctcgccctc gatgccacgc acgatcatcg
     9421 tcggccccta cctccgcggg cgcaccgagg tgctctcgca ggcgctgagc gaacggcgcg
     9481 gctccccggt tgaggtgcgc gcggccgagc gcggcgacaa acgcaagctg cgcgagctgg
     9541 ccgagcgcaa cgccaagctc gccctcgacc aggatcggct gcggcgcgag caccggcgcg
     9601 cgcgccgggt cgagtcgctc tcctcgctgc aggaagcgct ggggatggag gagctgccgg
     9661 tgcggatcga gggcttcgac atctccaaca tcggcggcga gcacaccgtc gcctcgatgg
     9721 tcgtgttcga ggggggtgcg accaagaagg ccgactaccg gcggttcaaa gtgcgcggcg
     9781 acctcaaagg ggagggcggc gccaacggcg gacccgacga cttcgcctcg atggaggagg
     9841 tcctgggccg gcggatgaac cgctacctcg aacaggccga cctatcgcca cacgacggca
     9901 aacgcgacgc cagcttcgcc tcgctgccct cgctgatcct gatcgacggc ggcaaagggc
     9961 agctcgctgc cgggatgcgg gcattggcgc cgtttgccga gcgcggcgtc accgtggtca
    10021 gcctcgccaa gcgcgaggag gaagtgttcg tccccgggcg ctcgcagccg ctcgacctgc
    10081 acgcgaacag cgagggctcg aagctgctgc agcgggtccg cgacgaggcc caccggtttg
    10141 cgatcgacca ccaccggggc cgccgcgacc aggcgatgac ggtctccgtg ctcgacgagc
    10201 tgaagggggt cgggccggcg cgcaagcgcg ccctgctcca gcacttcggc tcacccgagc
    10261 gcttcctcgc cgccacccgc gaggaactgg aggcggtgcc cggcctgcct ggcaaagtag
    10321 ggcgcgagat ccacgagcag ctgaacagaa tcggctgaag gcgacacatg accagaaggg
    10381 gcagatgtca gacgggaagg tgacgctggt ggtgattacc gggcactcgg gggccggtaa
    10441 gtcggaagcg atcgccgcct tcgaggacgg tggctacttc tgcgtcgaca acctgccgcc
    10501 gcggatgatc gagggcctcg gggagctctt ccgccacaaa ggcagcgggg tcgagcgcgc
    10561 cgcgatcgtc tccgacgtgc gcggcggcga gtacttcgac gacctgctgg cggtgctgga
    10621 tgacctcgac gccgacggcc tgaagccgac cgtgctcttc ctcgaggccg acgaggagac
    10681 cctgctcgac cgctacaagg agacccgccg ccgccacccg ctggcgccgg aagggcggat
    10741 cgtcgagggg atccgggccg agcacgagct gctggcgccg ctgcgcgagc gcgccgacat
    10801 ggtgatggac accagcgacc tgaccggggc cgagctgcgg cggcggatcg ccgaggagat
    10861 gctcgacggg gaggagggcg gcaagctggc gctgaccctg ctcaccttcg ggttcaagaa
    10921 cggcccgccg cgcgacgccg acctcaccct cgacgtgcgc ttcctgccca acccccatta
    10981 cgtcgacgag ctgcggccgc tgaccgggat ggacgaggtg gtgcgcacct acgtcgagtc
    11041 gggcacccag gcgggcgagt tctacgggcg gctgatgccc ttgctgcagt tcctcgttcc
    11101 cgcctacgtc gccgagggca agagccacct gacgatcgcg gtcggctgca ccggcggccg
    11161 ccaccgctcg gtcaccgttg ccgaccgcat ccgccgcgac ctcgccgaca tcgagggcgt
    11221 gatcgtgaaa gtcaaacacc gcgacttacc ggccgagtga gccgcgccgg ggcgcccgca
    11281 gcgccccgaa actgcttcat tcctagaatc cccccggctt tgccttcccc cgtcatctcc
    11341 atctccggcc tgcgcatgag ctacggcgag gtcgaagcgg tccgtggcat cgacctcgag
    11401 gtcatgcccg gcgagatctt cgccttcctc ggccccaacg gggccggcaa gacgacgacg
    11461 gtcgagatcc tcgaggggta ccgcaagcgc agcgggggag ctgtgagcgt gctgggggag
    11521 gatccgcagc gagccgggcg cgagtggcgc gagcggatcg ggatcgtgct gcagagcggc
    11581 cgtctcgacc cgtacctgac cgtgcgcgag tcgctcgggc tctacgcggg ctactaccgg
    11641 aatccgcggc cggtcgagga ggtgatcgcg ctggtcgggc tggaggagaa ggccgacacc
    11701 cgcgcgcgca agctctccgg cggccagcag cgccggctcg acgtcggcat ggccctggtc
    11761 ggcgaccccg agctgctctt cctc
//
LOCUS       DASRIG010000460         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_221337, whole genome shotgun sequence.
ACCESSION   DASRIG010000460 DASRIG010000000
VERSION     DASRIG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221337"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..521)
                     /locus_tag="VFB52_12325"
     CDS             complement(51..521)
                     /locus_tag="VFB52_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07166.1"
                     /translation="MDERKKLAYDESIRALSLQSVVLDDLRGRTGILLTAASLTATFI
                     GSRALDDGFTTWTRLALGFFLATGGFCLGVLWPRGQWGFAFNAKTILEGYIQQSNGAT
                     IEEMYVAFAEINQSNWEANADRLKWLYWSFRLAVLALVVQVPCWLIAIGSGQNG"
     gene            complement(557..853)
                     /locus_tag="VFB52_12330"
     CDS             complement(557..853)
                     /locus_tag="VFB52_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07167.1"
                     /translation="MPTRDVLASFWRDWDQLSPKQQRDFRAAVAQFIANLAEDKRGFH
                     PRLRVKRVQGHPGVWEMTWAYDGRATFQYGPEVTPGQPHIIWRRVGTHAIFRRP"
     gene            complement(840..1097)
                     /locus_tag="VFB52_12335"
     CDS             complement(840..1097)
                     /locus_tag="VFB52_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07168.1"
                     /translation="MTKAELHQMVDRLPDQAVDGAAILLREVTDGRIDPEQAWFWTRE
                     WQEREREADKDLAAGRVTRFESDEDFLADLDERTKPLDADA"
     gene            complement(1246..1319)
                     /locus_tag="VFB52_12340"
     tRNA            complement(1246..1319)
                     /locus_tag="VFB52_12340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1283..1285),aa:Gly,seq:gcc)
     gene            complement(1378..3042)
                     /locus_tag="VFB52_12345"
     CDS             complement(1378..3042)
                     /locus_tag="VFB52_12345"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZO07169.1"
                     /translation="MYDAPQHLTGGGARAGLLEWPDSRPLRSWQQQATEAVRAHSDAS
                     FLASATPAAGKTTFGLHIAHRMLSAGFVSRVVVAGPTTHICRQWAADAGRYGIDLEPN
                     RPNSDGPESTDFHGVAVTYQTIAAGPETHRLASSRRQTLLIADEPHHMGDQAAWGLQA
                     RKAFDGARFRLLLSGTPFRSDNSAIPWVTYDDDGVSRADYVYGYPQALVDRVCRPITF
                     LPYDGEMEWLSDGRLRSADFDLALPAAESARRLRTALLPEGEWMGEVLRDGDARLSEV
                     RAEGHPDAGGLVIASDQDHARAIAARLESVSGERPELVMSDEPGASQRIADFSASERR
                     WMVSVLMVSEGVDIPRLRVGVYATAARTELFFRQVVGRFIRTTPGGPRRQMSYLLMPA
                     DPRLKALAREIELERRHALDLAAEAEEDEGFDEPTPERGESGEGFAALSSGDAELDEA
                     IMSGTALQLFPTDDPKPVKKAAAAATTNGAPADREPERETAYAARERLRGERSKLVAN
                     VARRTNKSHREIQSWINRETRVRSVASATIEQLERGNAILRRELWG"
     gene            complement(3124..3501)
                     /locus_tag="VFB52_12350"
     CDS             complement(3124..3501)
                     /locus_tag="VFB52_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07170.1"
                     /translation="MGPDRDLTSKQIALVICGYLVATLGFFALLDNEFPGGGGLALGI
                     WTLASILLGVGTGRFAFALLAFAVIPISIPFGFADADEFAREPLPLWVASIYLAIYAW
                     IVVIVAVLTRLGVDYLRRRRAAS"
     gene            complement(3540..>4118)
                     /locus_tag="VFB52_12355"
     CDS             complement(3540..>4118)
                     /locus_tag="VFB52_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07171.1"
                     /translation="GVPEHLRTPAFALVPILIATWALGGLYMSLGPSVAAEVFDMPNH
                     VIGGLVVTLLCGTGALTIYALRNSTPARALTLATSWLVVGMVITLVGLAVEVAAITAL
                     GTMIAGVGFGASGRGTFGTIAALAGPEERGEVFAAFYVVSYLAFSLPALAAGIASTSA
                     GLQSTALVYGAVVLALTFAAIWAQRRLHLAFG"
BASE COUNT          684 a         1385 c         1447 g          602 t
ORIGIN      
        1 cacgcagccg ttctggtcgc tgcgatggtt ttcgttccgg ctttccctgt ttagccattc
       61 tgaccgctcc cgattgcgat gagccaacag ggtacctgca cgaccagcgc caaaactgcc
      121 agccggaagc tccagtacag ccacttgagc cgatccgcat tcgcttccca gttgctctgg
      181 ttgatctcgg cgaacgccac gtacatctcc tcgatggtgg cgccgttcga ctgctgtatg
      241 tagccctcaa gaatggtctt cgcgttgaac gcgaaacccc attgacccct tggccacaga
      301 acgcccagac aaaacccgcc cgttgcgaga aagaagccga gagccaaacg ggtccaggtc
      361 gtgaagccat cgtccagcgc ccgcgaaccg atgaacgtcg cggtcagcga ggcggcggtc
      421 aacaagatcc cggtgcgtcc ccgcaggtcg tcgagcacca cgctctgaag cgacaacgcc
      481 ctgatggatt cgtcgtaggc caatttcttg cgctcgtcca ttgcgcgatc agcttacggt
      541 cgcccgccgc gatcgactac ggacggcgaa agatcgcgtg cgtgccgacc cgtcgccaga
      601 tgatgtgcgg ctggccgggg gtgacctctg ggccgtactg gaaggtcgcg cggccatcgt
      661 aggcccaggt catctcccag acccccggat gcccctgcac ccgcttgacg cggagacggg
      721 gatgaaagcc ccgcttgtcc tcggcgagat tggcgatgaa ctgagccact gcggcgcgga
      781 aatcgcgctg ctgctttgga ctcagttgat cccaatcgcg ccagaacgac gcgaggacat
      841 cacgcgtcgg catcgagcgg cttcgtgcgc tcgtccaaat ccgctagaaa atcctcgtcg
      901 ctctcaaagc gagttacccg accggcggcg agatctttgt cggcttcccg ctcgcgttcc
      961 tgccactcgc gggtccagaa ccaagcctgc tcggggtcga ttcggccatc cgtcacctcg
     1021 cgaaggagaa tcgccgcgcc atcgactgcc tgatccggaa gccggtcaac catctgatgt
     1081 agctcagcct tggtcatgac gtaattctga catgccctcc ggaacagacg gcccgatggg
     1141 aaatggcagc gaacgggcag cgaagctggg aggcaaaggg cacagcgacg gtgtggccgc
     1201 cgcccggaca cggaaaagcc ccgccagagc ggggcttttc ctcagagcgg ctgatgggat
     1261 tcgaacccac gaccttctgc atggcaagca gacgctctag ccagctgagc tacagccgca
     1321 cgcagggcgg attctagctt tgcgccgacg ttcctgcctt ccgcgcccgg cggcggctca
     1381 gccccagagc tcgcggcgca ggatcgcgtt gccgcgctcg agctgctcga tcgtggcgct
     1441 ggcgaccgag cgcacccggg tttcgcgatt gatccagctc tggatctccc ggtgcgactt
     1501 gttggtgcga cgggcgacgt tggcgaccag cttgctgcgc tcaccgcgca gccgctccct
     1561 ggccgcgtag gccgtctccc gctcgggctc gcggtccgcg ggggcgccat tggtcgtggc
     1621 agcggcggcc gccttcttca ccggcttggg gtcgtcggtc gggaagagct gcagcgcggt
     1681 cccgctcatg atcgcctcgt cgagctcggc gtcgccggag ctgagcgcgg caaagccttc
     1741 gcccgactcg ccccgctccg gggtgggctc gtcgaacccc tcgtcttcct cggcttcggc
     1801 cgccaggtcg agcgcgtgcc ggcgctccag ctcgatctcg cgggcgagcg ccttcagccg
     1861 cgggtcggcg ggcatcagca ggtagctcat ctggcggcgc ggcccgcccg gggtggtgcg
     1921 gatgaagcga ccgacgacct ggcggaagaa cagctcggtc cgggccgccg tcgcgtagac
     1981 gcctacccgc aggcggggga tgtcgacgcc ctcggagacc atcaggaccg agaccatcca
     2041 gcgccgctcg gaggcggaga agtcggcgat ccgctggctg gcgccgggct cgtcgctcat
     2101 caccagctca ggtcgctctc ccgagaccga ctcgagccgc gccgcgatcg cccgcgcgtg
     2161 atcctggtcg gaggcgatca ccaggccacc ggcgtcgggg tgcccttcgg cccgcacctc
     2221 gctcagccgg gcatcgccgt cgcgcagcac ctcccccatc cactcaccct ccgggagcag
     2281 cgccgtgcgc aggcgccggg ccgactccgc cgccggcagg gcgaggtcga agtcggcgct
     2341 gcgcagccgc ccgtccgaga gccactccat ctcgccgtcg tagggcagga aggtgatcgg
     2401 gcggcagacc cggtcgacca gcgcctgcgg gtagccgtag acgtagtcgg cccggctgac
     2461 gccatcgtcg tcataggtga cccagggaat cgccgagttg tcggagcgga agggcgtgcc
     2521 cgagagcagc agccggaaac gggcgccgtc gaaggccttg cgggcctgca gcccccaggc
     2581 ggcctggtcg cccatgtggt gcggctcgtc ggcgatcaac agggtctggc ggcggctgga
     2641 ggccaggcgg tgggtctcag gcccggccgc gatcgtctgg taggtgacgg cgacaccgtg
     2701 gaagtcggtc gactccggcc cgtccgagtt cggccggttc ggctcgaggt cgatgccgta
     2761 acggcccgcg tcggcggccc actggcggca gatgtgggtg gtcggaccgg cgacgacaac
     2821 ccgcgagacg aagccggcgc tgagcatccg gtgggcgatg tgcaggccga aggtcgtctt
     2881 gcccgccgcg ggcgtagccg aggcgaggaa gctggcatcc gagtgcgcgc gcacggcttc
     2941 ggtggcttgc tgctgccacg aacggagggg tcgggaatcc ggccactcca gaagtccggc
     3001 acgcgccccg ccaccggtca ggtgctgagg agcgtcgtac aagggcgcgg aacaatatgg
     3061 tcggccgcgg cgggaggtcg gcccaccccg gaccggcttg gtctgcgccg ctggccggcg
     3121 gcgtcaggag gcggcgcggc ggcgccggag gtagtcgacg ccgagcctcg taagcacggc
     3181 gacgatgacc acgatccagg cgtagatcgc aaggtagatg gaggcgaccc agagagggag
     3241 cggctcgcgg gcgaactcgt ccgcgtcggc aaagccaaag gggatcgaaa tcgggatcac
     3301 cgcgaaggcc agcagcgcga acgcgaaccg gccggtcccc actccgagca gaatcgaggc
     3361 gagcgtccag attcctagcg ccagcccccc tccgcccggg aattcgttgt cgaggagcgc
     3421 gaagaaaccg agggtggcga ccaggtagcc gcagatgacg agcgcgatct gtttcgaggt
     3481 gaggtcccga tccggtccca ttgggcagaa acacaccgca acccgctggc ggttgcaact
     3541 cagccgaagg cgaggtgcag gcggcgctgg gcccagatcg cggcgaaggt gagggcgagg
     3601 acgacggcgc cgtagacgag cgcggtcgac tgcagcccgg ccgaggtgct ggcgatgccg
     3661 gcggcgaggg cgggcaggct gaaggcgaga taggagacga cgtagaaggc ggcaaagact
     3721 tcgccgcgct cttcgggacc ggcgagggcg gcgatcgtgc cgaaggtgcc gcggccggag
     3781 gcgccgaatc cgaccccggc gatcatcgtc cccagggcgg tgatcgcggc tacttcgacc
     3841 gcgaggccga ccagggtgat caccatcccg accaccagcc aactggtcgc gagcgtgagc
     3901 gcccgggccg gggtcgagtt gcgcagggcg tagatcgtca gcgccccggt gccgcagagc
     3961 agggtcacga ccaggccgcc gatcacgtgg ttgggcatgt cgaagacctc ggccgcgacc
     4021 gaggggccga gcgacatgta gaggccaccg agcgcccagg tcgcgatcag gatcggaacc
     4081 agggcgaacg cgggcgtccg caggtgctct ggcacgcc
//
LOCUS       DASRIG010000461         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2029586, whole genome shotgun sequence.
ACCESSION   DASRIG010000461 DASRIG010000000
VERSION     DASRIG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029586"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..974
                     /locus_tag="VFB52_12360"
     CDS             63..974
                     /locus_tag="VFB52_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO07172.1"
                     /translation="MGTPTILDPRIASGADYGNPDPTPAWMEVDWEAHQRRVELPGAT
                     VNYVEIGEGDPILFIHGISGGWQNWLENLPHFGQTHRAIALDLPGFGSSPMPEWDVDM
                     PSYGRMIHDFCERLGLQRTVLVGNSMGGFIAVEAATSEPQRYSRLVLVAAAGIMNTWN
                     PELRATVTAAAWSRFGGAVADRGHAIVAHPRSRQLALAPFFRYPNQLRPELLVEQMAG
                     GRCPGFGDALQSLISQDIRERLPSIEMPTLIVWGLQDRVIPVAAGISYHRRIPHSRLE
                     IFERTGHLPQLERPARFNALLDEFLSP"
     gene            complement(964..2658)
                     /gene="ilvD"
                     /locus_tag="VFB52_12365"
     CDS             complement(964..2658)
                     /gene="ilvD"
                     /locus_tag="VFB52_12365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZO07173.1"
                     /translation="MSSNGNTTTALPRPRSATVFDGPDRAHSRAYMKGIGFDDDALKR
                     PTIGIANTWTEAMPCNFHLRGLAEHVKEGVRAAGGTPMEFNTVAVSDGVTMGTQGMKA
                     SLISREVIADSIELMARGYQFDAIIALCACDKTIPGCAMGLARLDVPSVLLYGGSIAP
                     GHWHGRDVTILDIFEAIGAHNAGDMTDEELNELEGVASPGAGACGGQFTANTMACAFE
                     ALGISAGGSAMVPAEDDEKQTVAEKVGELVLKVLVDDLRPSKILTRDALENAIACVCA
                     SGGSTNAVLHLIALAHEVGIELTMDDFERISRETPLFADLKPGGRFVATDLYAAGGVP
                     VILSRLAGKGLLHEDAITVTGQTIGEVAASATEAPGQEVVRALDDPLKAEGGLAILRG
                     NLAPDGSVVKVAGTERRQQTGPARVFETEEDCFRAVKEQQINPGDIVVIRNEGPVGGP
                     GMREMLQVTAAIVGEGLGESVAMVTDGRFSGATRGLMIGHVAPEAAKGGPIAAVREGD
                     EITVDIDSRSLSVALSDEEIASRVAEYVSPESPFGRGVMAKYAATVSSAAQGAVTG"
     gene            complement(2675..3496)
                     /gene="thiD"
                     /locus_tag="VFB52_12370"
     CDS             complement(2675..3496)
                     /gene="thiD"
                     /locus_tag="VFB52_12370"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZO07174.1"
                     /translation="MSSARIPAVLSIAGSDSGGGAGIQADLKAFARCGVHGMTAITAI
                     TVQNTVEVRVVEAVAPETIVAQVRAVAEDIGVDAVKIGMLGSAETVDAVVEALSYVGD
                     APVVIDPVMVAESGATLLDPEARAALIERLLPLTSIATPNIPEARELTGLGEGVDQES
                     LARQVFALGPRVAVVVTGGHSENLVDLFFDGEQVVEIPGERHPDGAAHGSGCTHSSAL
                     AAFLARGADPLQAAAAAREVAAVAVGAGLREIGQGAGPVDVFELSRRANQEADLS"
     gene            3565..4254
                     /locus_tag="VFB52_12375"
     CDS             3565..4254
                     /locus_tag="VFB52_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZO07175.1"
                     /translation="MRDELILALDLYFQKGRNPGGEAAQQLSETLRAIPVESWRTDDP
                     KFRSPQSVTYKLQNFVALDPSLATGGFSHGGKGDAEVWEEFSSDPERLHKVALSIRDN
                     LGSLTASEAEESEESITEAPEGQVLTRMHRKRERSASLRKAKKETVQKATGRLACEAC
                     DMDFSERYGTRAKGFIECHHIVPLHTLKPGQRTRLEDLALLCSNCHRVLHLRKPWLSV
                     DELKQLINTPA"
     gene            complement(4255..>5133)
                     /gene="dprA"
                     /locus_tag="VFB52_12380"
     CDS             complement(4255..>5133)
                     /gene="dprA"
                     /locus_tag="VFB52_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZO07176.1"
                     /translation="WACCRHGELYPAQLREAADAPWALIGRGDPGMIAGLEPFSAVTI
                     VGARRATSYGREIARELGRELAGAGMTVVSGLAFGIDGCAHRGALDADGRTIAVLGCG
                     PDNAYPAAHRSLWRRIGEVGLVLAEQPPGAPPWRWTFPARNRIMAALAGMTVVVEGAA
                     RSGSLITADLAADLGRDLGAVPGPVTSRASAGPNNLLAGGACLVRSAQDVLDAMLGAG
                     AQRIVRTGPPLDEPLTAVLTAVEAGETTCDAVATALDLSAPDTAAALARLEAFGYLTC
                     SPLGTYARTLLVAEAS"
BASE COUNT          787 a         1818 c         1808 g          720 t
ORIGIN      
        1 aaccctgaaa cggttcgcca aagacgtgat ggtgcgacgc cacagcacca agcggtaggc
       61 cgatgggcac gccgacgatc ctcgacccgc ggatcgcctc cggcgccgac tacggcaacc
      121 ccgacccgac accggcctgg atggaggtcg actgggaggc gcaccagcgc cgcgtcgagc
      181 tgccgggggc aaccgtcaac tacgtcgaga tcggcgaggg cgacccgatc ctcttcatcc
      241 acgggatctc cggcggctgg cagaactggc tcgagaacct cccccacttc ggccagaccc
      301 accgggcgat cgcgctcgac ctgcccgggt tcggcagcag cccgatgccc gagtgggacg
      361 tcgacatgcc gagctacggg cggatgatcc acgacttctg cgagcgcctc ggcctgcagc
      421 gcacggtcct ggtcggcaac tcgatgggcg gcttcatcgc ggtcgaggcg gcgacgtcgg
      481 agccgcagcg ctacagccgc ctggtcctgg tcgcggcggc ggggatcatg aacacctgga
      541 acccggagct gcgggcgacg gtcaccgccg ccgcctggag caggttcggc ggcgccgtcg
      601 ccgaccgggg ccacgcgatc gtcgcccacc cgcgcagccg ccagctagcg ctggcgccgt
      661 tcttccgcta cccgaaccag ctgcggccgg agctgctggt cgagcagatg gccggcggcc
      721 gctgccccgg cttcggcgac gcgttgcaga gcctgatcag ccaggacatc cgcgagcgcc
      781 tgccgagcat cgagatgccg accctgatcg tctggggcct acaggaccgg gtgatcccgg
      841 tcgccgccgg gatcagctac caccgccgca tcccccactc ccgcctggag atcttcgagc
      901 gcacgggcca cctgccgcaa ctcgagcggc cggcacgctt caacgccctg ctcgacgaat
      961 tcctcagccc gtaaccgcgc cctgagcggc ggagctcacg gttgccgcgt acttggccat
     1021 cacgccgcgg ccgaaggggg actcgggtga gacgtactcg gccacgcggc tggcgatctc
     1081 ctcgtcggag agggcgacgc tgaggctgcg cgagtcgatg tcgacggtga tctcgtcgcc
     1141 ctcgcggacg gcggcgatcg gaccgccttt ggcggcctcg ggcgcgacgt ggccgatcat
     1201 caggccgcgg gtggcgccgg agaagcgacc gtcggtgacc atcgcgacgc tctcgccgag
     1261 gccctcgccg acgatcgcgg cggtgacctg gagcatctcg cgcatccccg ggccgccgac
     1321 cgggccctcg ttgcggatca cgacgatgtc gcccgggttg atctgctgtt ccttgaccgc
     1381 gcggaagcag tcctcttcgg tctcgaagac gcgggccgga ccggtctgct ggcggcgctc
     1441 ggtgccggcg accttgacca ccgagccgtc gggggcgagg ttgccgcgca ggatcgcgag
     1501 gccgccttct gccttcagcg ggtcatcgag cgctcgcact acttcctgtc ccggtgcctc
     1561 ggtcgcgctc gccgcgacct cgccgatcgt ctgcccggtg accgtgatcg cgtcctcgtg
     1621 cagcagccct ttgccggcga ggcggctgag gatcaccggc acgccgccgg cggcgtagag
     1681 gtcggtggcg acgaagcggc cgcccggctt caagtcggcg aagagcgggg tctcgcggga
     1741 gatccgctcg aagtcgtcca tcgtcagctc gatcccgacc tcgtgcgcga gcgcgatcag
     1801 gtgcaggacc gcgttggtcg agccgccgga ggcgcagacg caggcgatcg cgttctcgag
     1861 cgcgtcgcgg gtgaggatct tgctcgggcg caggtcgtcg accagcacct tcagaaccag
     1921 ctcgccgact ttctcggcga cggtctgctt ctcgtcgtct tcggccggga ccatcgccga
     1981 gccgccggcg ctgatcccca gcgcctcgaa cgcgcaggcc atcgtgttgg cggtgaactg
     2041 gccgccgcag gcaccggcgc cggggctggc cacaccctcc agctcgttca gttcctcgtc
     2101 ggtcatatcg ccggcgttgt gggcgccgat cgcctcgaag atgtcgagga tggtgacgtc
     2161 gcggccgtgc cagtggccgg gggcgatcga gccgccgtag agcagcaccg agggcacgtc
     2221 gagccgggcc aggcccatcg cgcagccggg gatcgtcttg tcgcaggcgc agagcgcgat
     2281 gatcgcgtcg aactggtagc cgcgcgccat cagctcgatc gagtcggcga tcacctcgcg
     2341 gctgatcagc gaggccttca tcccctgggt gcccatcgtc accccgtcgg agacggccac
     2401 cgtgttgaac tccatcggcg tgccgccggc cgcgcgcacg ccctccttga cgtgctcggc
     2461 gaggccgcgc aggtggaagt tgcagggcat cgcctcggtc caggtgttgg cgatcccgat
     2521 cgtcgggcgc ttcagcgcgt cgtcgtcgaa cccgatcccc ttcatgtagg cgcgcgagtg
     2581 cgcgcggtcg gggccgtcga agacggtggc cgagcgcggc cggggcaggg cggtggtggt
     2641 gttgccgttc gagctcatag gacaaatgta ttcatcagga caagtccgct tcctggttgg
     2701 cgcgcctcga gagctcaaaa acgtccaccg ggccggcgcc ctggccgatc tcgcgaaggc
     2761 ccgccccgac ggcgaccgcg gcgacttcgc gggccgcggc ggcggcctgc agcgggtcgg
     2821 cgccgcgggc gaggaaagcg gccagggccg aggagtgggt gcagccggag ccgtgcgcgg
     2881 cgccgtcagg atgcctctcc cccggaatct caaccacctg ctcgccatcg aagaagaggt
     2941 cgacgaggtt ctcgctgtgt ccgccggtga cgaccaccgc cacccgcggg ccgagcgcga
     3001 acacctgccg cgccagcgac tcctggtcga ccccctcccc cagcccggtc agctcccgcg
     3061 cctcggggat gttcggcgtc gcgatcgagg tcaacggcag cagccgctcg atcagcgccg
     3121 cccgcgcctc cggatcgagc agcgtcgccc cgctctcggc gaccatcacc gggtcgatca
     3181 ccaccggcgc gtcgccgacg taagagagcg cctcgaccac cgcgtcaacc gtctctgcgc
     3241 tgcccagcat cccgatcttg accgcgtcga ccccgatgtc ctcggcgacc gcccgcacct
     3301 gcgcgacgat cgtctccggc gccaccgctt cgaccacccg cacctcgacc gtgttctgca
     3361 ccgtgatcgc cgtgatcgcg gtcatcccat gcaccccaca acgcgcaaac gccttcagat
     3421 ccgcctgaat cccggccccc ccgccggagt cggagccggc gatcgagagc actgcgggaa
     3481 ttcgcgcgct ggacatgacc gggagcctag atacccaggc gacggaggta gtctctcctc
     3541 atgccgacgc gggggcgagc atggatgcga gacgagctaa tcctggcgct cgacttgtat
     3601 ttccagaaag ggagaaatcc cggcggggag gcagcacagc agctcagcga aacgttgcgc
     3661 gcgatcccgg tcgaatcttg gcgcaccgac gatccgaagt ttcgcagtcc gcaatcggtc
     3721 acctacaagc ttcagaactt cgtggccctc gatccctcgc tggccacggg tgggttttcc
     3781 cacggaggaa aaggcgacgc agaagtctgg gaagagtttt cgtccgaccc ggagcgcctg
     3841 cacaaagtcg ccctctcaat ccgagacaac cttggctccc tgactgcaag cgaggccgaa
     3901 gagagcgagg agtcgatcac cgaggcaccc gagggtcaag tcctgacccg catgcaccgc
     3961 aaacgcgagc gcagcgcaag ccttcgaaag gcgaagaagg agacggtgca gaaggccacg
     4021 gggcgcctcg cttgcgaggc gtgtgacatg gacttcagcg agcgctacgg aacgagggcg
     4081 aagggattca tcgagtgcca ccacatcgtc ccgctgcaca cgctcaaacc gggtcagcgc
     4141 actcgcctcg aggatctagc gcttctctgc tccaactgcc accgcgtcct ccacctccgt
     4201 aagccttggc tttccgtaga cgagctgaag caactgatca acaccccggc ctgactacga
     4261 agcctcggct accagcaacg tgcgggcgta ggtgccaagc ggggagcagg tgaggtagcc
     4321 gaaggcctcg aggcgggcga gggcggcggc ggtgtcgggg gcggagaggt cgagggcggt
     4381 ggcgacggcg tcgcaggtgg tctcgccggc ttcgacggcg gtgagcacgg cggtgagggg
     4441 ctcgtcgagg ggagggccgg tgcggacgat gcgctgggcg ccggccccga gcatcgcgtc
     4501 gagcacgtcc tgggcgctgc ggacgaggca ggcgccgccg gcgagcaggt tgttggggcc
     4561 ggcggaagcg cgcgaggtga ccgggccggg gacggcgccg aggtcgcggc cgaggtcggc
     4621 cgccaggtcg gcggtgatca gcgagcccga gcgggcggcg ccctcgacca cgacggtcat
     4681 cccggcgagc gccgccatga tccggttgcg ggccgggaag gtccagcgcc agggcggcgc
     4741 cccgggcggc tgctcggcga gcaccaggcc gacttcgccg atccgccgcc agagcgagcg
     4801 gtgcgcggcc ggataggcgt tgtcgggacc gcagccgagc accgcgatcg tccgcccatc
     4861 ggcgtcgagg gcgccgcggt gggcgcagcc atcgatcccg aaggcgaggc cgctgaccac
     4921 ggtcatcccg gcgccggcca gctcgcgacc gagctcccga gcaatctcgc ggccgtagga
     4981 ggtggcgcgg cgggcgccga cgatcgtcac cgcggagaac ggctccagcc cggcgatcat
     5041 gcctgggtcg ccgcgaccga tcagcgccca gggcgcgtcg gcggcctcgc ggagctgcgc
     5101 cgggtagagc tcgccgtgcc ggcagcaggc cca
//
LOCUS       DASRIG010000462         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3082354, whole genome shotgun sequence.
ACCESSION   DASRIG010000462 DASRIG010000000
VERSION     DASRIG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082354"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1190
                     /locus_tag="VFB52_12385"
     CDS             219..1190
                     /locus_tag="VFB52_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO07177.1"
                     /translation="MVDPSTPTEAPAGDARPPEFGSHYFQHACGVPYERNEHWLKFFG
                     GVAEGIVRDLHPTSTLDAGCAMGFLVEELRKRGVDASGVDLSEYAISQAHESVAEHVR
                     VGSLGEPLGRRYDLIVCIEVLEHIPAAEADAAIANLCAASDRILLSTTPENFGEASHV
                     NVRPPEAWSAALAREGLFRDLERDFSYITPWAALYTKVEEPPSETVRRYDRAWWRLRR
                     EATEVRAALLTNEERLAELSASGAEDRDELQRELDASKEEVLRLRDLLIGKDAELGAA
                     KGHLAELEAQSHGLISLARQVQARVPRLTRLLGAVLRRLQGRRGNAE"
     gene            1187..2848
                     /locus_tag="VFB52_12390"
     CDS             1187..2848
                     /locus_tag="VFB52_12390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO07178.1"
                     /translation="MSAPRFSVVTPVYDTPADVLWAMLESVRAQSFGDWELCLVDDRS
                     PSPHVREMLERVAAEEPRIKVRWRDQNGGIVAASNDALEMANGEFVALLDHDDELHPD
                     ALAHVHEALLGNPEADYVYTDEDKIDRGGHHSGPFFKPDWSPERMRTQMYTCHLSVMR
                     RSLVAEVGGFDGEFEGSQDWDLVLRVTERARAVLHVPRVLYHWRMLETSTAGSGEAAK
                     PWAFEAGTRAIQAQCDRTAIEARAVRDEDDAGVYHLEPALRSEPLVSIVIPTGGTVRE
                     VRFEPVVLVEHCVRSIVETSTYSNYELVVVVDADTDPAISARLREIAGERLRLVTYDR
                     PFSFSAKINLGAVRSKGEHLLLLNDDMEVVTPDWLERMVMYSQMDGIGAVGGRLLWED
                     GRLQHVGIRFHEGLPGHPHYGFSREYRGYGNAVRVANNCLAVTGACLMTRRELFEAVG
                     GLSTRLPVNYNDVDYCLKLREVGKRTVYDPDLVLYHFESSSRSSEVEDWEKDLFRQRW
                     LPLTAVDPYSNPNLRHETPRLSSPFRWAVRRRPRLRRRRLKGQPA"
     gene            2850..3494
                     /locus_tag="VFB52_12395"
     CDS             2850..3494
                     /locus_tag="VFB52_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO07179.1"
                     /translation="MNFERLYSYRFREIDQDARLAVWKEIGPHIYKLMERPQKVVDPA
                     AGRGEFIGSIAAPETWAVDAVAYPEAAHKPDTNVVVSRIMDAELPSDHFDGVFVSNFL
                     EHLHSQAEIADFLAKMHGHMEKGGRIAILGPNYRYCAREYWDYADHYVPLTHLAVCEH
                     LYAAGFEPQNAIARYLPYSFRGSLPPSPKLTRLYLKTPAAWRVLGKQFLVIGRK"
     gene            3495..4427
                     /locus_tag="VFB52_12400"
     CDS             3495..4427
                     /locus_tag="VFB52_12400"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZO07180.1"
                     /translation="MAGPRILVVGCGFIGSHVVAELVGRGQPPAVLTRSEPQPALATA
                     GLRPRIGDAASRAVVDAVLEDVEHVVYSAGGLLPAASEEDPERDAELTLAPIRTVLAA
                     LRERPGVGLTYLSSGGTVYGEPETVPVPEDAPANPIGAYGRLHLECEDEVLREQREHG
                     LAVRILRCASVYGPYQEPDRGQGAATTFLHRIEAGLPIDLYGDGKSERDYVYAGDVAG
                     VVAELAGREDGPAVLNVGSGEGTSLLDLLRAAEREVGRPAEVVTHPARGFEVRRVVLD
                     VARLRALTGFEPTPLAEGIAHTHEWLSATARQRV"
     gene            4424..>5271
                     /locus_tag="VFB52_12405"
     CDS             4424..>5271
                     /locus_tag="VFB52_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07181.1"
                     /translation="MNERLAERLHGLRWRWTAMKRLWGPGARPDMPFLEPERLPSEPI
                     GALELPEDEAALGRALEVEGWVLFPSGPAARVELTLGGRPLGLARLAIPRHDVRAAWD
                     SPHGPLSGFALRASMDEWGGPAGPAQLTAVATSLAGERFELEPVSVNVVEEEAPDTRL
                     APPAQRTSLAGPPPAGKPRVLVITHQLGLGGAQLYLLDLLREMVAAERAGFTVVSTYD
                     GALRPQLEALGIPVHVSGVTSWEDFSRHIGAVEELVSWLSEREFDAALVNTTTALSIP
                     GAEAAE"
BASE COUNT          764 a         1961 c         1845 g          701 t
ORIGIN      
        1 ccggtggcgg cgagggcgat cgcgaggatg accgggacgt tgcgcacgcc ccagcgcgcc
       61 gccagcaagg ccgccgcgac cccgggcaag ccggttagta ggagctggca gaccaggacc
      121 agcagcagat caccctgcat cgcgcgacag gtttgcacaa cgactggagg gcacgccgcg
      181 agcgggaatc ggcccgattg gtgggtagac tcgccccgat ggtcgatccg tcgacgccga
      241 ccgaggcgcc agctggggac gcgcgcccgc ccgaattcgg ctcgcactac ttccagcacg
      301 cctgcggcgt cccctacgag cgcaacgagc actggctgaa gttcttcggc ggggtcgccg
      361 aggggatcgt gcgcgacctg cacccgacct cgacgctcga cgccggctgc gcgatgggct
      421 tcctggtcga ggagctgcgc aagcgcgggg tcgacgcctc cggggtcgac ctctccgagt
      481 acgcgatctc ccaggcgcac gagtcggtcg ccgagcacgt ccgggtcggc agcctcggcg
      541 agccgctggg ccgccgctac gacctgatcg tctgcatcga ggtgctcgag cacatccccg
      601 ccgccgaggc cgacgcggcg atcgccaacc tctgcgccgc cagcgaccgg atcctgctct
      661 cgaccacgcc ggagaacttc ggcgaggcca gccacgtcaa cgtgcgcccg cccgaggcct
      721 ggtcggcggc gctcgcccgc gaaggcctct tccgcgacct cgagcgcgac ttctcctaca
      781 tcaccccctg ggccgccctc tacaccaagg tcgaggagcc gccgagcgag accgtgcgcc
      841 gctacgaccg cgcctggtgg cggctgcgcc gcgaggcgac cgaggtgcgc gcggcgctgc
      901 tcaccaacga agagcggctg gccgagctga gcgcgagcgg ggccgaggac cgcgacgagc
      961 tgcagcggga gctggacgcc agcaaggagg aggtgctgcg cctgcgcgat ctgctgatcg
     1021 gcaaagacgc cgaactgggc gcggcgaagg gccacctggc cgagctcgaa gcccaatcgc
     1081 acggcctgat cagcctggcg aggcaggtgc aggcgcgggt gccgcgcctg acccgcctgc
     1141 tcggcgccgt ccttcgccgg ctgcagggcc ggcgggggaa cgctgagtga gcgcgccccg
     1201 cttctcggtc gtcaccccgg tctacgacac gccggccgac gtgctctggg cgatgctcga
     1261 gtcggtgcgc gcgcagagct tcggcgactg ggagctctgc ctggtcgacg accgctcccc
     1321 ctccccgcac gtgcgcgaga tgctcgagcg ggtcgccgcc gaagagccgc ggatcaaggt
     1381 ccgctggcgg gaccagaacg gcggcatcgt cgccgcctcc aacgacgccc tggagatggc
     1441 gaacggcgag ttcgtcgccc tgctcgacca cgacgacgag ctccaccccg acgcgctcgc
     1501 ccacgtccac gaggcgctgc tcggcaatcc cgaggccgac tacgtctaca ccgacgagga
     1561 caagatcgac cgcggcggcc accactccgg ccccttcttc aagcccgact ggtcgccgga
     1621 gcggatgcgc acgcagatgt acacctgcca cctgagcgtg atgcggcgca gcctggtcgc
     1681 cgaggtcggc ggcttcgacg gcgagttcga gggctcccag gactgggacc tggtcctgcg
     1741 ggtgaccgag cgtgcccgcg ccgtgctgca cgtgccgcgc gtgctctacc actggcgcat
     1801 gctcgagacc tcgaccgcgg gctcgggcga agcggcgaag ccgtgggcct tcgaggccgg
     1861 cacgcgagcg atccaggcgc agtgcgaccg caccgcgatc gaggcccgcg ccgtccgcga
     1921 cgaggacgac gccggcgtct accacctgga gccggcgctg cgctccgagc cgctggtcag
     1981 catcgtgatc ccgaccgggg gcacggtgcg cgaggtccgc ttcgagccgg tcgtcctggt
     2041 cgagcactgc gtgcgcagca tcgtcgagac ctcgacctac tcgaactacg agctggtcgt
     2101 cgtcgtcgac gccgacaccg acccggcgat ctccgcccgg ctgcgcgaga tcgccggcga
     2161 gcgcctgcgc ctggtcacct acgaccggcc gttcagcttc tcggccaaga tcaatctcgg
     2221 cgcggtgcgc agcaaaggcg agcacctgct gttgctgaac gacgacatgg aggtcgtcac
     2281 gccggattgg ctggagcgga tggtgatgta ctcgcagatg gacggaatcg gcgcggtcgg
     2341 cggccgcctg ctctgggagg acgggcgcct gcagcacgtc ggcatccgct tccacgaagg
     2401 cctgcccggg cacccccact acggcttctc gcgcgagtac cgcggctacg gcaacgcggt
     2461 ccgcgtcgcc aacaactgcc tcgccgtgac cggcgcctgc ctgatgaccc ggcgcgagct
     2521 gttcgaagca gtcggcgggc tctcgacccg gctgccggtc aactacaacg acgtcgacta
     2581 ctgcctgaag ctgcgcgagg tcggcaagcg caccgtctac gaccccgact tggtcctgta
     2641 ccacttcgag tcctcctcac ggtcctcgga ggtcgaggac tgggagaagg atctgttccg
     2701 ccaaaggtgg ttaccgttga ccgccgtcga cccctactcg aaccccaacc tgcgccacga
     2761 gacgccccgg ctcagctccc cgttccgctg ggcggtacgg cggcggcccc ggctgcgccg
     2821 gcgccggctg aaaggacaac ccgcctgacg tgaacttcga gcgcctgtac agctaccgct
     2881 tccgtgagat cgaccaggac gcccgcctcg cggtctggaa ggagatcggg ccgcacatct
     2941 acaagctgat ggaacgaccc cagaaggtcg tcgacccggc cgccggccgc ggcgagttca
     3001 tcggctcgat cgccgccccg gagacctggg cggtcgacgc ggtcgcctac ccggaggcgg
     3061 cgcacaagcc ggacaccaac gtggtcgtct cgcggatcat ggacgcggag ctccccagcg
     3121 accacttcga cggggtgttc gtctccaact tcctcgagca cctgcacagc caggccgaga
     3181 tcgccgactt cctcgccaag atgcacgggc atatggagaa gggcgggcgg atcgcgatcc
     3241 tcggtccgaa ctatcgctac tgcgcgcgcg aatactggga ctacgcggac cactacgtgc
     3301 cgctcaccca cctggcggtc tgcgagcatc tctacgcggc cgggttcgag ccgcagaacg
     3361 cgatcgcccg ctatctgccc tactcgttca ggggctcgct ccccccctcg ccgaagctga
     3421 cccggctcta cctgaagacg ccggccgctt ggcgggtcct cggcaagcag ttcctggtca
     3481 tcggccgcaa gtagatggcg ggcccgcgga tcctcgtcgt cggctgcggg ttcatcggct
     3541 cccacgtcgt cgccgagctg gtcggacgcg gacagccgcc ggcggtgctg acccgctccg
     3601 agccgcagcc cgcccttgcc accgcggggc tgcgaccccg gatcggcgac gccgccagcc
     3661 gcgcagtcgt cgatgcggtg ctggaagacg tcgagcacgt cgtctacagc gccggcggcc
     3721 tgctccccgc cgcctccgag gaagatccgg agcgcgacgc cgaactgacc ctggcgccga
     3781 tccgcacggt gctggcggcg ctgcgcgagc gccccggggt cggcctcacc tacctctcct
     3841 ccggcggcac cgtctacggc gagccggaga ccgtgccggt ccccgaggac gccccggcca
     3901 atccgatcgg cgcctacggc cgcctccacc tcgagtgcga ggacgaagtt ctgcgcgagc
     3961 agcgcgagca cggcctcgcg gtgcggatcc tgcgctgcgc cagcgtctac ggcccctacc
     4021 aggagcccga ccgcggccaa ggcgcggcca ccaccttcct gcaccggatc gaggcggggc
     4081 tgccgatcga cctctacggc gacggcaaga gtgaacgcga ctacgtctac gccggcgacg
     4141 tcgccggcgt ggttgccgag ctggccgggc gcgaggacgg cccggcggtg ctcaacgtcg
     4201 gctccggcga gggcacctcg ctgctcgacc tgctgcgcgc ggccgagcgc gaggtcgggc
     4261 gtccggccga ggtcgtcacc catccggcgc gcggattcga agtccgccgt gtggtgctcg
     4321 acgtcgcgcg cctgcgggcg ctgaccgggt tcgagccgac gccgctcgcc gaggggatcg
     4381 cccacaccca cgagtggctg agcgccacgg cgaggcagcg ggtatgaacg agcgcctcgc
     4441 cgagcggctg cacgggctgc gctggcgctg gaccgcgatg aagcggctct gggggccggg
     4501 cgcgcgtccc gacatgccct tcctcgagcc cgagcgcctg ccaagcgagc cgatcggcgc
     4561 gctcgaactg cccgaggacg aggcggcgct gggacgggcg ctggaggtcg agggctgggt
     4621 gctcttcccc tccgggccgg cggcgcgggt cgagctgacg ctcggcggcc ggcccctggg
     4681 cctggcccgg ctggcgatcc cgcgccacga cgtgcgcgcc gcctgggact cgccgcacgg
     4741 gccgctcagc ggcttcgccc tgcgcgccag catggacgag tggggcggcc cggccgggcc
     4801 ggcgcagctg accgcggtcg cgaccagcct cgccggcgag cgcttcgagc tggagccggt
     4861 gagcgtgaac gtggttgagg aggaggcgcc agacacgcgg ctggcaccgc cggcgcagcg
     4921 caccagcctg gccggcccgc ccccggccgg caagccgcgg gtgctggtga tcacccacca
     4981 gctcggcctc ggcggcgccc agctctacct gctcgatctg ctccgcgaga tggtcgccgc
     5041 cgagcgggcg ggcttcaccg tggtcagcac ctatgacggc gccctgcgcc cgcagctcga
     5101 agcgctcggg atccccgtgc acgtctccgg cgtcacctcc tgggaggact tcagccgcca
     5161 catcggcgcg gtcgaggagc tggtctcctg gctctccgag cgcgagttcg acgccgccct
     5221 ggtcaacacc acgacggcgc tctcgatccc cggcgccgag gccgccgagc a
//
LOCUS       DASRIG010000463        21241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1525548, whole genome shotgun sequence.
ACCESSION   DASRIG010000463 DASRIG010000000
VERSION     DASRIG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525548"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFB52_12410"
     CDS             complement(<1..387)
                     /locus_tag="VFB52_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07182.1"
                     /translation="MNPTEQTGAGEFAGRVAIVTGAAGGIGRATALRLAASGAAVLVA
                     DRREEVAETGAAIKAAGGTALHRIIDVTDDEQVRGMVGAARERFGRLDVLVNNAGAVG
                     RPAPLAELDPEAFDRTLAINARGTFLG"
     gene            479..1252
                     /locus_tag="VFB52_12415"
     CDS             479..1252
                     /locus_tag="VFB52_12415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07183.1"
                     /translation="MPAQLEDKIAVVTAGGSGIGRATSLLFAAEGAKVAVVDIDGEAA
                     DETARQIAAAAGECSAHAVDCADVGALERLFADIDRRYGRVDVLFNHAGTPNAHGVAE
                     VEEREFDRAVDLNLRSGFFATRFALPLMTREGRGGAIIFTASTSGLMGSETSPLYSMT
                     KHGVVGMVRSMAQLLAPEGIRVNAVCPGPVRTPMLAGFMNLGAEETDAVAEHYGSATA
                     LKRVAEPEEIAAAVLFLACDAASYVTGVPLPVDAGFVAK"
     gene            1277..2170
                     /locus_tag="VFB52_12420"
     CDS             1277..2170
                     /locus_tag="VFB52_12420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO07184.1"
                     /translation="MAERVKGNWGRWGEEDERGALNLMTPESVLAALRVPSSGKLYTL
                     GLPIQRTGVPNVDYRGIPARLTMVNHEDESMFEPFGGTPGTGTNEDVLMMASHTVTHM
                     DALCHIYNEGAIYNGFRGDEVSPYGGAKHCGIENAGGVLARGVLIDVAGQKGVEWLEP
                     GYVITVEDVEAALEAQGTELRPGDAVIFRTGWLEWFFANEEMSLVQPGIGLDLSDYLA
                     ARDPVVVCADNTAVEAQPFDREEFLGCHVELLVRRGIHLIEHVKTEELAADGCYEFLF
                     SVSPLLVTGATASPVSPIAIG"
     gene            2250..3998
                     /locus_tag="VFB52_12425"
     CDS             2250..3998
                     /locus_tag="VFB52_12425"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07185.1"
                     /translation="MGNRELANLRPEIRRSWERSLMCGLDPGAVIDLPYEPELGAEER
                     FLRAAGPVLGHVGGFLDGARTTAVLTDPRGRVLLRNCPDSELARVLDRGQSVPGFAWG
                     EEFAGTTALSLAIEEHLPAMVSGGEHFLEALRHLTCAAAPIVHPISQRLVGVIDVTSE
                     TGSASAHMMPVVLQAAQAIQERLYGEASTVERVLLSYFLGAANRSGRPIVVLGERVEL
                     STPPAARLLDVDDRTLLWEHAAELIDGTVPASKTLTLSDGRELQATFTPIHDDERGVG
                     VAVEIVAEPAPVGGVLAGAAPASPARAAAPSAATASFLGRSASAQYLREQAEAIRGEL
                     MPVLVSGEPGVGKLSLARCLTGDGMDSVLFDAATTSVDGEAGLIREVANVAKTPGKTV
                     IVRRIGCLSAEAVQMLIGLAASAEENESRLVATATAASELAERNPAAELVESFGLRLD
                     LPPLRERADDIVDLVPHFVRRRGATARVAPAAIQALMRYDWPGNARELDSLVRALLTR
                     KRTTDIVLADLPPVYQQGSRRLRRIEQIERAAIVRALIEANGNKTQAAELLEIGRATL
                     YRKIRAYGLDLELTTR"
     gene            complement(4013..4465)
                     /locus_tag="VFB52_12430"
     CDS             complement(4013..4465)
                     /locus_tag="VFB52_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07186.1"
                     /translation="MTIDAEQIRALTHEYTFRLDGGDFAGVAALLAAGALRMSAKGMD
                     EQEMRGAEEIERFYANQVVTHDGDPRTRHVISNHVVDIDGEGAHGRCYFTVLMKPPRQ
                     PIQIVVCGRYFDTFERHEGAWRFLRKVIQVDYLTDISEHFLIDADHAA"
     gene            4574..5569
                     /locus_tag="VFB52_12435"
     CDS             4574..5569
                     /locus_tag="VFB52_12435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07187.1"
                     /translation="MSEPSMSFGVFYDFRNPAQFREDWTKRYREIFEQIDWVERDSNF
                     NAVSVSEHHFVDDGYTPSVLALATAIAAKTERVEIGTNIIQLPLHNPLRIAEDSLTVD
                     ALSGGRFRLGIAIGYRGLEFEGLGSNVKERRTRMEEGIEILRAAFAGEPFSHRGRHWS
                     FPELRVTPLPVREGGPPIWMGGTVPAALERAARLGDGFLASVDEEVAGYLEACERNGV
                     DPATRGTCRTAWAIIDEDPERALAELGPYMLHQVNGYVDFGFLKVPYFEHAQELVDAG
                     FYTMVDADGAVEYFRDSWDHGVRETHLFAQIPGEPVESGTRRLQYVSEQVIPRLS"
     gene            5692..6813
                     /locus_tag="VFB52_12440"
     CDS             5692..6813
                     /locus_tag="VFB52_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07188.1"
                     /translation="MPKVLVAGASGVVGNAVVERFLARDRFEVVALSRRRPEVASAKE
                     FQHLTVDLREREAARTALDGAAGITHLVFAALSEQPGLISGWSDRAQMELNLSLLQNV
                     LDPLLEAGSLRHVILMQGTKAYGSHLHPIPVPARERAPRDDHENFYWLQEDYLLDQAN
                     RRGVSWTILRPQLIVGRAWGVAMNMTPVLGAYAALRRAEGEPFSFPGGSDYVTEAADA
                     RLVAEVIEWSLAAPNAVNEVFNVTNGDVFSWRAVWPRIAKTLGVETAPDSPLEIHEYL
                     GAKDEEWAQLCEADGLRPIPLPELLGEAHHLADYIFAHGAVEAPPPGFSSRVKLNQAG
                     FGSCYDTADTFSYWLEDLIGQRVIPGPMQNAAAPEGAGV"
     gene            6810..7832
                     /locus_tag="VFB52_12445"
     CDS             6810..7832
                     /locus_tag="VFB52_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07189.1"
                     /translation="MTRDRLTAVTPLKVISVGVLVMLVVSELVISGSLFAQSTLSTIL
                     APLSILIVIAIGQALVIGTGGIDLSVPATVTLTGVIILKASEGKDSGLLKALLIVAGA
                     CIVIGLVNGLLVEGLRLNALVVTLATGQLILGFMRLYGGELLPASEVPPQLSAWTADD
                     IVGGVSYLFVIAAVVAVLGTLFLQRFVQGRRLVASSASERSAFLAGIAARRYRILAYV
                     LGSLCYGLGGVLASGDVGTPDLTLGDPYLLLSIVAVILGGAAVTGGRASPIATMLGAI
                     FIFLLDYDLRVKGVSGGLREAVQGAVLVLGLSLVFLIQNMPTVRAALARLRGGSSLPS
                     DQTGAA"
     gene            7903..9165
                     /locus_tag="VFB52_12450"
     CDS             7903..9165
                     /locus_tag="VFB52_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO07190.1"
                     /translation="MLVLVLGIVAAGCGGDDDTTAGGAGDTTAASTEETTTGDETGGG
                     EISPDAEKLASKALGVEYSDGALDENGIEAFEKAVETQDLTPEQEDLAYECWSQSTCT
                     LGDGELTYGVADGFGGNTWRKFTKMEGILQAMAYPDIGKYIYLDAEFDLAKMQSNIRS
                     LSAQGADIITGFNDFGSAVVPAYAAAQRAGAVVSVYVGPVEDADSSAITSQVVTEWCE
                     AGKNMADAAAEILSDKGSIALFGGPPGNTQGETWWRCAEEQIGAEYPDMSVTYKGDTE
                     WTAASAAKAASAAIASGKPIDLIMYDYAEPVVNIVETYKKAGKTPPAFVAVNQSNGLV
                     TNWSEAQGSKSEYALDMTSSLAYMQRISITVAVNKINGEEVPEEIFPPLDLIPAAKDQ
                     VEPDKPSDYPGPTIIVPEGLTERMLTSG"
     gene            9261..11897
                     /locus_tag="VFB52_12455"
     CDS             9261..11897
                     /locus_tag="VFB52_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO07191.1"
                     /translation="MRESRSAPAAPGVPAALEVQGISKSYGVVRALKPASLVLRPGEI
                     HALVGENGSGKSTFVGVVSGTVRPDTGAVAVGGQPLGRHSPAESQRNSVLTVFQDGSI
                     LPQLTIAQNLYLGTPANLRPSYSRHEKWAAELLAEWELGRIDVRAPAEGLSPGDRQSL
                     EIVRAVAAGPGVLLLDEATSALDSAGVDGALALMQRAAAAGSAVMFVTHRLSEVFRVA
                     ERISVLRDGEWQGTYAVDGVDTDGLVELMAGTKVNTEFPPQATADELGEVVLSAENLT
                     GVGFGPVGLQVRAGEIVGIAGADDNGQIGFLRGLGRIGAPTGPLSVGGRSIASYKQAV
                     DAGATYLSSDRANESLFHALPIRENLAAGMLGPLSRLGLVSWRGERELVDDTVETFGI
                     RLSSPEAPVASLSGGNQQKVALGRVMATHPKVLLVDEPTQGVDVRSRIDIYNQLRDGA
                     RRGLAVVMVSSDASELAGLCDRVVVMSRGKLVAELATEEATEESIVNSFTALGHGAGE
                     ETLAAVAAGEESAPAAGEPAAPRLSAWERVRSVVRRNENGPRLGLLALLLVLIGAYAQ
                     SENDVFLSHANVYNLLFLAVPLAAIAAAEFAVLYVGEIDVSVGAVMALMIVLMSEFVQ
                     SGSFVVALLLSLLIALIIGVVVGLGNAALVEGARISPIIATIGTLGIIGGVGLMIRPT
                     AGGSISTDLLNALAEEVWVLPWSFVVLVVLFVLGDWALRSTGAGLRLRSVGLNAAFAH
                     RLGVNARLVRTAAFVVASFFAACAGLLLSAQIGIGDATVGQGYLLLAIAAPVLGGASL
                     AGGHGTFVGCLLGACLLVTVQNLTTLLGIKDAYSYLLTGGLTILALLLYTSGAGAAIR
                     NYVRSLRLRFGGGAPGEAGAKA"
     gene            11894..12625
                     /locus_tag="VFB52_12460"
     CDS             11894..12625
                     /locus_tag="VFB52_12460"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HZO07192.1"
                     /translation="MTAVKVHDLFAHREDLSREEFFAYWRSAHAGLATTFKQIKHYTQ
                     AHRSGDQDLVPGFAETWPDGCAETWYDDASEVDAMVAEPRFAAELMEDEKNFMNLENP
                     RPLLVSELSLLDENGFDPEDRGIKVLIFAARPGGTSREEFLAAWTSDDDAALGHELGA
                     TRQALYTPVTTGGVLIQTDLEDGSHQEGGGSYDAVREVWWKDRAGLEAATAEDNEPWR
                     RLLRKDALDTSRSITLIARENVVLP"
     gene            12661..13794
                     /locus_tag="VFB52_12465"
     CDS             12661..13794
                     /locus_tag="VFB52_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZO07193.1"
                     /translation="MFAGSSNRVGIVASAQTQFKRAWADRQHVDLIGEVVNAALKGSG
                     LRIEDVDFVIDSGSDILDGRSISNCGFLGAMGAHQKEESRVEEDGLWSALYGATKIGA
                     GSAEVGLLVAYSKPSESDVAAFYWSQCEPFYQRPVGFGQAAASGLQAQSYLAEHGLSE
                     EIFADVAAAEWAKAVANPALEIGDAPSAAEVRESGNAAAPLRELEMSQPVDGAVAVVI
                     ASQRVAERICPDPVWITGIGTAMDQHAFSRRQRSALPAAAVAAEGAYKRAGIDGPQGL
                     SLAEVSASSSATQLMVLEALGLAEAGKGAALYEDGSKVAINPSGGALPADPIMATGLA
                     RLSEAALQLTGRVGHAPEGASSAVVHGAGGVGMQNHCVFTLEV"
     gene            13796..14956
                     /locus_tag="VFB52_12470"
     CDS             13796..14956
                     /locus_tag="VFB52_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07194.1"
                     /translation="MPRDVAIVGTGQTVYARRRTDVSQAGLVREAVDAALEDAELTFA
                     DVDSIVVASGPALFGSVNQPEKWLVDGLGGLMKPVVRITSGGGTGLAGALAAMNQVSS
                     GFAERVLVVAYDKLSEGALQYQISTLYDPFWGREFAVGIMGFASAYWRARIETLGHTE
                     EAAAMVGVKNHRNAFLNPYAHVRKEITVEDVLASRPLAWPIKLLDVPPISDGACAVVL
                     ASPEAAEKTTAKPAWIRGMSYFGEADNAPDRFMLQSVPLQLAAQRVYKKAGITNPRRQ
                     FDVAELQEPYTCFELSYYEGLGLCPTGEAAKLIESGATDMEGDIPCNPSGGCMGANPI
                     GATALIRLAEAALQVTDKAGERQIEGARTALVQAGGGWANLRGVAVVGAERN"
     gene            14967..15935
                     /locus_tag="VFB52_12475"
     CDS             14967..15935
                     /locus_tag="VFB52_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZO07195.1"
                     /translation="MESSAVEKMAMRGSGLGSTPNDNPTFAGKIDLPYTLTTGRAAAT
                     FLAELANKKIAGSRCSECKEVTVPAQDFCAACGTTLEELVEVSASGELAGFTETAEGL
                     IGLIRLDGAANEFPHRLLDTEFGSLSVGDRVQVRWAAEPEGSILDIEGFEPGGEPGGE
                     VVPLEASAEPVLEQKYGMRLDYEHAYGPYYGMLFDGLATSRRIQGSRCPSCTRVLVPP
                     REYCDVCYVRTGEWVDVADTGTIKAFSIIHLEFVGQTREPPYVYAEIVLDGSATRLIH
                     TVGGIDIEEAKERLRIGTKVRAVWKEGGEPVGTLEDIDHFEPLFEE"
     gene            15938..16726
                     /locus_tag="VFB52_12480"
     CDS             15938..16726
                     /locus_tag="VFB52_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO07196.1"
                     /translation="MAGFEMVELEVADGLATVTLNRPDALNALNVQLKDELAEVWRRL
                     GPDPEVRAVLLTGAGRAFSTGGDIKQMDPDRTPEATRVMIAKLGREVVIPLAQLEKPV
                     VAAVNGHAHGLGLALALACDVIYAAESAVLSAAFIRIGLSPDGCCSYLLTRTVGILAA
                     KELMFSGRRIDGIEAQQLGLVTQVVAGAELLGTAREAALGFAAAPTIALARTKRLLNE
                     AAQHTIEETAELEALAQAVARGADDHAEGVAAFAEKRPATFTGS"
     gene            16730..17653
                     /locus_tag="VFB52_12485"
     CDS             16730..17653
                     /locus_tag="VFB52_12485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO07197.1"
                     /translation="MPAYDELPVIEKLGMPHAWDALDHDLGTLDRGTPEQVTAAAELV
                     REGTVLTLNLPLNEPDPPLFGRDPIRQEIFATDRNTLDDRFDAFYPQGSTQWDGLRHV
                     RAREFGFFGGLTDEFEPGDERLSITHWARRGIVGRGVLLDLPAHRERRGEPWDPFAAE
                     PIQAEELEAIVAGQGVELKPGDVLCLRTGWIDAYRELDRAGRERAAAEVAISGLAGSE
                     AVARFLWDSGAIAIAADNPTLEVAPGDPAVGSLHRRLIPLLGFAVGELLDLGVLAGHC
                     AGDSRWDFMFVSVPLNLPGGVGSPANAMAIR"
     gene            17650..18843
                     /locus_tag="VFB52_12490"
     CDS             17650..18843
                     /locus_tag="VFB52_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07198.1"
                     /translation="MNTSVKTLPSSVLGPAISVAPEDHPFRQEFRAWLADNVPGVPEP
                     LDQDEKFQMRRAWQRTLFEGGWAGPAWAEEYGGRGAGPLHQFMYYEELALARAPFIAN
                     TPGISLLGPTLIEVGSDELKSRFLPVMLSSEEIFSQGFSEPGAGSDLAAIRARAKRDG
                     EEWVINGQKIWTTWAQYSDYCFVLCRTDPESERHRGLSLLICPVDQPGVTVREIEQIS
                     GDPEFAEVFFDDARCPASWVVGEVDDGWSTAMILFQFERGDQGFTDHSRILVSLHDLA
                     AELREASQRESISRPSVEAARRRLADLWVRAQQLRRINLRIAVRQEAGESIGMEGSVV
                     NIFWGELERDLGELTAAVHGPRGLLLGTDPGHEVLAARAGTIYSGTSEIQRNIIAERM
                     LGLPR"
     gene            18846..19856
                     /locus_tag="VFB52_12495"
     CDS             18846..19856
                     /locus_tag="VFB52_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07199.1"
                     /translation="MSIPATDTPLRRSLVFATDELEPLPELARRCEAGGLDRVWTTEY
                     VGRDAIARALAVALATERIEVATGITYAFTRVPLAMAALAADVQRLSGERFALGISSG
                     TRGVRRWYGAEFEPPAPALAEYAGALREAWGKIPELQQPPKVYGAVLNPIMTRHVARS
                     CDGALLHALALSRTHLRERLLPALAKGLEQREDPRPLEIAAWCITSIDEDEEQARENA
                     RRQLAFYLSTPSYRTVAEGTSWEQVAGEVREAYEASDRKAAWGDLSHLIPDHVVDELT
                     ISGTPASAPERAAAMEAELAAAGVTELVFQTAGADVPAAELISSCEQIADVLGADSKV
                     SK"
     gene            19853..20968
                     /locus_tag="VFB52_12500"
     CDS             19853..20968
                     /locus_tag="VFB52_12500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07200.1"
                     /translation="MTTTVTLSPEQDEIRGVARSFLEARYPSDRVRELMKTPAGFEQS
                     DWDEIAELGWTGIAVPEGQGGAGYSDVERCLLLEEMGRVLFPCPFLSSAVLAADALAL
                     AGEGGAAAELLPGVVDGSTRATLVAGGDLGAGQGSALRAVARGEEHELSGNGGLTLDG
                     HTGELLIVVAALEGGELALFAVDPGAEGVIRNPVETIDETRKTAVVEFERTPAHRLDG
                     GDGGEGIALALDRGAVALAAEMAGGARKAIEMTVAYMHEREQFGGPIGRFQALKHRLA
                     DLHVRIDVARQSVYAAADALAAGESEHVPMLAAAAKYAASIGYVTATAEAIQLHGGIG
                     FTEEHDIGLYYKRALVSAETLGAPADQVERIALGLDV"
     gene            20961..>21241
                     /locus_tag="VFB52_12505"
     CDS             20961..>21241
                     /locus_tag="VFB52_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZO07201.1"
                     /translation="MSEPAAPMPEPDVFDRLYFEDMEVGRGWVSPRRTIGEGDVSAFA
                     GLSGDFNPLHVDAVFAAETAFGKRLVHGALVFAIATGLRQQMPIFRGSMR"
BASE COUNT         3155 a         7646 c         7321 g         3119 t
ORIGIN      
        1 cccgaggaag gtgccgcggg cgttgatcgc gagggtgcga tcgaacgcct cggggtcgag
       61 ttcggcgagc ggcgccggcc ggccgacggc gccggcgttg ttgaccagca catcgaggcg
      121 cccgaagcgc tcgcgcgcgg ccccgaccat gccccgcacc tgctcatcgt cggtcacgtc
      181 gatgatgcgg tgcagggccg tgccgccagc tgccttgatc gcggcgccgg tctcggcgac
      241 ctcctcgcga cggtcggcga ccagcaccgc ggcgccggag gcggcgagtc gcagcgcggt
      301 ggcgcggccg attccgccgg ctgcgccggt gacgatcgcg acccggccgg cgaactcgcc
      361 ggcgccggtc tgctcggtcg ggttcatcgg ccgagcgtag gccccgcctc cgccgtgatg
      421 ccgcaggtgt ttcgtactga gacggctcgg gccctgcggc gtgctggatc ctggccaggt
      481 gcccgctcag ctcgaggaca agatcgccgt tgtcaccgcc ggcggctcgg gaatcggccg
      541 cgccaccagc ctcctgttcg ccgccgaggg cgcgaaggtg gccgtcgtcg acatcgacgg
      601 cgaggcggcc gacgagaccg cgcgccagat cgccgcggcc gctggcgagt gctcggcgca
      661 cgcggtcgac tgcgccgatg tcggcgccct cgagcgcctc ttcgccgaca tcgaccggcg
      721 ctacggccgc gtcgacgtgc tcttcaacca cgctgggacc cccaacgccc acggcgtcgc
      781 cgaggtcgaa gagcgcgagt tcgaccgcgc cgtcgacctc aacctgcgca gcggcttctt
      841 cgcgaccagg ttcgcgctgc cgctgatgac gcgcgagggg cgcggcggcg cgatcatctt
      901 caccgcttcg acctcgggcc tgatgggatc ggagaccagc ccgctctact cgatgaccaa
      961 gcacggggtg gtcgggatgg tgcggtcgat ggcgcagctg ctcgcccccg aggggatccg
     1021 ggtcaacgcc gtctgcccgg gcccggtacg gaccccgatg ctggccgggt tcatgaacct
     1081 cggcgccgag gagaccgacg cggttgccga gcactacggc agtgccacgg cgctgaagcg
     1141 ggtcgccgag ccggaggaga tcgccgccgc ggtgctcttc ttggcctgcg acgcggcgag
     1201 ctatgtgacc ggggtgccgc tgccggtcga cgccggtttc gtggccaagt gaagttcgaa
     1261 tggaagcgag ggtgagatgg ctgagcgcgt aaagggcaac tggggccggt ggggtgagga
     1321 ggacgaacgc ggtgcgctca acctgatgac cccggagagc gtgctggcgg cgctgcgcgt
     1381 ccccagcagc ggcaagctct acacgctcgg gctgccgatc cagcggaccg gcgtgcccaa
     1441 cgtcgactac cgcgggatcc cggcgcggct gacgatggtc aaccacgagg acgagagcat
     1501 gttcgagccc ttcggcggga cgccggggac cgggaccaac gaggacgtgc tgatgatggc
     1561 ctcgcacacg gtcacccaca tggacgcgct ctgccacatc tacaacgaag gcgcgatcta
     1621 caacggcttc cgcggcgacg aggtctcacc ctacggcggc gccaagcact gcgggatcga
     1681 gaacgccggc ggcgtgctcg cccgcggggt gctgatcgac gtcgccggcc agaagggggt
     1741 cgagtggctg gagcccggct acgtgatcac ggtcgaggac gtggaggcgg cactcgaggc
     1801 gcagggcacc gagctgcgcc ccggcgacgc ggtgatcttc cgcaccggct ggctcgagtg
     1861 gttcttcgcc aacgaagaga tgtccttggt tcagccgggg atcggcctcg acctatccga
     1921 ctacctggcc gcgcgcgacc cggtcgtcgt ctgcgccgac aacaccgcgg tcgaggcgca
     1981 gccgttcgac cgtgaggagt tcctcggctg ccacgtcgag ctgctggtcc gccgcggcat
     2041 ccacctgatc gagcacgtga agaccgagga gcttgccgcc gacggttgct acgagttcct
     2101 gttctcggtc agcccgctgc tggtgaccgg ggcgacggcc agcccggtca gtcccatcgc
     2161 aatcggctga ccgctcaacg gtaaccaaac aaccgtttag ttatagtcgg cgcagaggag
     2221 aagtacccgg agtacgagga gagaccttta tggggaaccg cgagctcgcg aacctgcgtc
     2281 cggagatccg ccgctcctgg gagcgttcgt tgatgtgcgg gctcgacccg ggcgccgtga
     2341 tcgaccttcc ctatgagccg gagctggggg ccgaagagcg gtttctgcgc gccgccgggc
     2401 cagtgctcgg ccacgtcggg ggttttctcg acggtgctcg cacgaccgcg gtcctcaccg
     2461 acccgcgcgg ccgggtgctg ctgcgcaact gcccggactc agagctggcc cgggtgctcg
     2521 accgcggcca gagcgtcccc ggcttcgctt ggggcgagga gttcgccggc acgactgcgc
     2581 tcagcctggc gatcgaggag cacttgccgg cgatggtcag cggcggcgag cacttcctcg
     2641 aggcgcttcg ccacctgacc tgcgcggcgg ccccgatcgt ccacccgatc agccagcgcc
     2701 tggtcggggt gatcgacgtg accagcgaga ccggcagcgc cagcgcccac atgatgccgg
     2761 tggtgctgca agcggcgcag gcgatacaag agcgcctcta cggggaagcc tcgacggtcg
     2821 agcgggttct gctctcctac ttcctgggtg ccgccaaccg cagcggtcgg ccgatcgtgg
     2881 tgctcggcga gcgggttgag ctgagcaccc cgccggccgc gcggctgctc gacgtcgacg
     2941 atcgcacgct gctttgggag cacgccgcgg agctgatcga cggcacggtt ccggccagca
     3001 agacgctgac cctgagcgac ggccgcgagc tgcaggcgac cttcaccccg atccacgacg
     3061 acgagcgcgg ggtcggcgtc gcggtcgaga tcgtcgccga gcccgcgccc gtgggcgggg
     3121 tgctggccgg tgccgcaccc gcgtcgcccg cccgcgccgc ggccccgagc gcggcaaccg
     3181 cgtccttcct cggtaggtcc gcctccgcgc agtacctgcg cgagcaggcg gaggcgattc
     3241 ggggcgagct gatgccggtc ctcgttagcg gcgagcccgg ggtcggcaag ctgtcgctgg
     3301 cccgctgcct gaccggcgat gggatggaca gtgtcctctt cgacgccgcc accacctcgg
     3361 tcgacggcga ggcaggcctg atccgcgagg tcgccaacgt cgcgaagacg cccggcaaga
     3421 cggtgatcgt gcgtcggatc gggtgcctct cggcggaggc cgtgcagatg ctgatcgggc
     3481 tcgcggccag cgccgaagag aacgagtcgc gactggtcgc gaccgcgacc gccgcttcgg
     3541 agctggccga gcgcaacccg gccgccgagc tggtcgagtc cttcggcctg cgcctcgatc
     3601 tgcccccgct gcgcgagcgc gccgacgaca tcgtcgatct cgtgccccac ttcgtgcggc
     3661 gccgcggggc gacggcgcgg gtcgcgccag ccgcgatcca ggcgttgatg cgctacgact
     3721 ggccgggcaa cgcgcgcgag ctggactcac tggtgcgggc gctgctgacc cgcaagcgca
     3781 ccaccgacat cgtcctcgcc gacctgccgc cggtctacca gcagggcagc cgccgtctgc
     3841 gccggatcga gcagatcgag cgggccgcga tcgtgcgcgc cctgatcgag gccaacggca
     3901 acaagaccca ggccgccgaa ctgctcgaaa tcggccgcgc caccctctac cgcaagatcc
     3961 gcgcctacgg cttggatctg gagctgacga cgcgctgagc gggcggcccc ggctaggcgg
     4021 cgtggtcggc gtcgattagg aagtgctcgg agatgtcggt gaggtaatcg acctggatca
     4081 cttttcggag gaagcgccag gcgccttcgt ggcgctcaaa ggtgtcgaag tagcggccgc
     4141 agacgacgat ctggatcggc tggcggggtg gcttcatcag caccgtgaag tagcagcggc
     4201 cgtgggcacc ctcgccgtcg atgtcgacca cgtggttgct gatcacgtgg cgggtgcggg
     4261 gatcgccgtc gtgggtgacg acctggttgg cgtagaagcg ctcgatctcc tcggcgccgc
     4321 gcatttcctg ctcgtccatc cccttcgccg acatccgcag cgcgcccgcg gcgagcaggg
     4381 cggcgacgcc ggcgaagtcg ccgccgtcga gccggaaggt gtactcgtgg gtcagggcgc
     4441 ggatctgttc ggcatcgatc gtcatcgccg cccagcctgc caggccacgc cgttgggtcg
     4501 cgtatcgagt tgagacagat cgccgcgccg tccggggaga ggatcgagtc gtccttctac
     4561 ctgggagcga ggcatgtccg aaccatcgat gtcgttcggg gtcttctacg acttccgcaa
     4621 cccggcccag ttccgcgagg actggaccaa gcgctaccgc gagatcttcg agcagatcga
     4681 ttgggtcgag cgcgactcga acttcaacgc cgtctcggtc tccgagcacc atttcgtaga
     4741 tgacggctac acgccctcgg tgctggccct agcgacggcg atcgcggcga agacggagcg
     4801 ggttgagatc ggcaccaaca tcatccagct gccgctgcac aacccgctgc ggatcgccga
     4861 ggactcactc accgtcgacg ccctctccgg cggccgcttc cgcctcggga tcgcgatcgg
     4921 ctaccgcggg ctcgagttcg aagggctcgg ctccaatgtg aaggagcggc gcacgcggat
     4981 ggaggagggg atcgagatcc tccgcgccgc cttcgccggc gagccgttct cgcaccgggg
     5041 ccgccactgg tccttcccgg aactgcgggt gacgccgctg ccggtgcgcg agggcgggcc
     5101 gccgatctgg atggggggga cggtgccggc ggcgctcgag cgcgccgccc ggctcggcga
     5161 cgggttccta gcctcggtcg acgaggaggt ggccggctac ctcgaggcct gcgagcgcaa
     5221 cggggtcgac ccggccacgc gcggcacctg ccgcactgcc tgggcgatca tcgacgagga
     5281 ccccgagcgc gccctcgccg agctcggccc ctacatgctg caccaggtca acggctacgt
     5341 cgacttcggc ttcctcaagg tgccgtattt cgagcacgcc caggaactgg tcgacgccgg
     5401 cttctacacg atggtcgacg ccgacggggc ggtcgagtac ttccgcgatt cctgggacca
     5461 cggcgtccgc gaaacccacc tgttcgcgca gatccccggc gagccggtcg agtccgggac
     5521 gcggcgcttg cagtacgtct ccgagcaggt gatcccgcgg ctctcttgac aaaccagcca
     5581 aacgtacggt aggttcccaa ccaaccgttt ggttatgcat tggattcggg ttgaatcgag
     5641 tcgccggccc gtggggggcc gagaggagag aggtttgaaa caggagcgga gatgcccaag
     5701 gtcctggtag ccggcgccag cggtgtcgtc ggcaacgctg tcgtcgagcg cttcctcgct
     5761 cgtgaccggt tcgaggtcgt ggcgctctcg cgccgccgcc ccgaagtcgc ctcggcgaag
     5821 gagtttcaac acctcaccgt cgaccttcgc gagcgcgagg cggcgaggac ggcgctcgac
     5881 ggcgccgccg gcatcaccca cctcgtcttc gcggcgctca gcgagcagcc cggcctgatc
     5941 agcggctgga gcgaccgcgc ccagatggag ctcaacctct cgctgctgca gaacgtgctc
     6001 gacccgctgc tggaggcggg ctcgctgcgc cacgtgatcc tgatgcaggg gacgaaggcc
     6061 tacggcagcc acctgcaccc gatcccagtc ccggccaggg agcgggcacc gcgcgacgac
     6121 cacgagaact tctactggct gcaggaggat tacctgctcg accaggcgaa ccggcgcggg
     6181 gtcagctgga cgatcctgcg cccgcagctg atcgtcggcc gcgcctgggg ggtggcgatg
     6241 aacatgaccc cggtgctcgg cgcctatgcc gccctgcgcc gcgccgaagg cgagccattc
     6301 tccttccccg gcggcagcga ctacgtgacc gaggcggccg acgcccgcct cgtcgcggag
     6361 gtgatcgagt ggtcgctggc ggccccgaac gcggtcaacg aggtcttcaa cgtgaccaac
     6421 ggcgacgtct tctcctggcg cgcggtctgg ccgcggatcg cgaagacgct cggggtcgag
     6481 accgcccccg actccccgct cgagatccac gagtacctcg gtgccaagga cgaggagtgg
     6541 gcacagctct gcgaggccga cggcctgcgc ccgatcccgc tcccggagct gctcggcgag
     6601 gcccaccacc tggccgacta catcttcgcc cacggcgccg tcgaggcgcc gccgccgggc
     6661 ttctccagcc gggtgaagct gaaccaggcc ggcttcggca gctgctacga caccgccgac
     6721 accttcagct actggctcga agacctgatc ggccagcggg tgatcccggg gccaatgcag
     6781 aacgcggcag cgccggaggg cgctggggta tgacccgaga ccggctcact gcggtaacgc
     6841 cgctgaaggt gatcagcgtc ggcgtcctcg tcatgctcgt cgtctccgag ctggtgatca
     6901 gcggcagcct cttcgcccag agcaccctct cgacgatcct cgcgccgctc tcgatcctta
     6961 tcgtcatcgc gatcggccag gcgctcgtga tcgggaccgg cgggatcgac ctcagcgtcc
     7021 cggcgaccgt gacgctgacc ggggtgatca tcctcaaggc ctccgaaggc aaggacagcg
     7081 gcctgctcaa ggcgctcctg atcgtcgccg gcgcctgcat tgtgatcggg ctcgtcaacg
     7141 gcctcctggt cgaggggctg cggctgaacg cgctggtcgt cacccttgcc accggccagc
     7201 tgatcctcgg cttcatgcgc ctctacggcg gcgagctgct gcccgcctcc gaagtgccgc
     7261 cgcagctctc ggcctggacc gccgacgaca tcgtcggcgg cgtctcctac ctgttcgtga
     7321 tcgcggccgt cgtcgccgtg ctcggcacgc tcttcctgca gcgcttcgtc cagggccgcc
     7381 gcctggtcgc cagcagcgcc tccgaacggt cggccttcct tgccgggatc gcggcgcgcc
     7441 gctaccggat cctcgcctac gtcctcggct cgctctgtta cgggctcggc ggcgtcctcg
     7501 ccagcggcga cgtcggcacc cccgacctca cgctcggcga cccctacctg ttgctcagca
     7561 tcgtcgccgt gatcctcggc ggcgccgccg tgaccggcgg ccgggccagc ccgatcgcga
     7621 cgatgctcgg ggcgatcttc attttcttgc tcgattacga cctccgcgtc aagggcgttt
     7681 ccggaggttt gcgagaagcc gtgcaaggcg ccgtgcttgt gctcggtctg agccttgtct
     7741 tcctgatcca gaacatgccc acagtaaggg ccgcgttggc gcggttgagg ggcggcagtt
     7801 cgctgccctc cgaccaaacc ggggctgcct aagaggagga gagagcgtta catgcgcaat
     7861 aacagtcgaa acagccgatg gcggcttggc ttcctggcca tgatgttggt tctcgtgctc
     7921 ggcatcgtcg ccgccggctg cgggggcgat gacgacacca ccgccggcgg tgctggcgac
     7981 actactgccg ccagcaccga agaaaccact accggcgatg aaaccggggg cggtgaaatc
     8041 tcccccgacg ctgaaaaact ggcctccaag gcgctcggag tcgaatactc ggacggagcg
     8101 ctcgacgaga acgggatcga agcgttcgaa aaagcagtcg aaacgcagga tctcaccccc
     8161 gagcaggaag acctcgccta cgagtgctgg tcgcagtcga cctgcacgct gggcgacggt
     8221 gaactgacct acggcgtcgc tgacgggttc ggtggcaaca cctggcggaa attcaccaag
     8281 atggaaggaa tcctccaggc gatggcctac ccggacatcg gcaagtacat ctacctggat
     8341 gcggaattcg acctcgcgaa aatgcagtcg aacatccgca gtctcagcgc ccagggcgcc
     8401 gacatcatca ccggcttcaa cgacttcggc tccgccgtcg ttccggcata cgccgcggcg
     8461 cagcgcgccg gcgccgtggt ctcggtgtac gtcggcccgg tcgaagacgc cgactcctcg
     8521 gcgatcacca gccaggtcgt gaccgaatgg tgcgaagccg gcaagaacat ggccgacgcc
     8581 gccgccgaaa tcctcagcga caaaggcagc atcgccctgt tcggtggtcc tcccggcaac
     8641 acccagggtg aaacctggtg gcgctgcgcc gaagaacaga taggtgccga atacccggac
     8701 atgagcgtca cctacaaggg tgacaccgaa tggaccgccg cctcggccgc gaaagccgcc
     8761 tcggccgcga tcgcgtccgg caaaccgatc gacctgatca tgtacgacta cgcggaaccc
     8821 gtagtcaaca tcgtcgaaac gtacaaaaaa gcgggcaaaa ccccgccggc gttcgtcgcg
     8881 gtgaaccaga gcaacggact cgtcaccaac tggtccgaag cccagggcag caaaagcgaa
     8941 tacgcgctcg acatgacgtc ctcgttggcg tacatgcagc ggatctcgat caccgttgcg
     9001 gtgaacaaaa tcaacggcga ggaagtgccg gaagagatct tcccgccgct tgacctgatc
     9061 cccgcggcca aagaccaggt cgaaccggac aaaccgagtg actacccggg cccgaccatc
     9121 atcgttccgg aaggactcac cgaacggatg ctgacgtccg gctgatccac ccgccgaccg
     9181 ctggtcccgg ccgccgccct tgggtggccg ggaccgccgg ttcccgccga gatcttctta
     9241 tgaacgagga acacacgaac ttgagagagt cccgcagcgc gcccgccgcg cccggagttc
     9301 ccgccgcgct cgaggtccag gggatctcga agagctacgg ggtggtcagg gcgctgaagc
     9361 cggcctcgct tgtcctccgg ccgggcgaga tccacgccct ggtcggcgag aacggctccg
     9421 gcaagtcgac cttcgtcggc gtggtcagcg gcaccgtccg ccccgacacc ggcgccgtcg
     9481 cggtcggggg ccagccgctc ggccgccaca gccccgccga gtcccagcgc aacagcgtcc
     9541 tcactgtctt ccaggacggc tcgatcctgc cgcagctgac gatcgcccag aacctttacc
     9601 tcggcacccc cgccaacctg cgcccgtcct actcgcgcca cgagaagtgg gcggcggagc
     9661 tgctggccga gtgggagctg gggcggatcg acgtccgtgc ccccgccgag ggcctctcgc
     9721 ccggcgaccg ccagagcctc gagatcgtcc gcgccgtcgc cgccgggccg ggcgtgctgc
     9781 tgctcgacga ggcgacctcg gcgctcgact ccgccggcgt cgacggggcg ctggcgctga
     9841 tgcaacgcgc cgccgccgcc ggctccgcgg tgatgttcgt gacccaccgt ctctccgagg
     9901 tcttccgcgt cgccgagcgg atctcggtcc tgcgcgacgg cgagtggcag gggacttatg
     9961 ccgtcgacgg cgtcgacacc gacggcctgg tcgagctgat ggccgggacc aaggtcaaca
    10021 ccgagttccc gccgcaggcc accgccgacg agctcggcga ggtcgtgctc tcggccgaga
    10081 acctcaccgg ggtcggcttc gggccggtcg gcctccaggt tcgcgccggc gaaatcgtcg
    10141 gcatcgccgg cgccgacgac aacggccaga tcggcttcct ccgcgggctc gggcggatcg
    10201 gcgctccgac cggcccgctc agcgtcggcg gccgctcgat cgccagctac aagcaggcgg
    10261 tcgacgccgg cgccacctac ctctccagcg accgcgccaa cgagtcgctc ttccacgccc
    10321 tgccgatccg cgagaatctc gccgccggga tgctcgggcc gctctcgcgg ctcggtctgg
    10381 tcagctggcg tggcgagcgc gagctggtcg acgacaccgt cgaaaccttt gggatccgcc
    10441 tctcctcgcc cgaggcccct gtcgcctcgc tctccggcgg caatcagcag aaggttgccc
    10501 tcggacgggt gatggcgacc cacccgaagg tgctgctggt cgacgagccg acccagggcg
    10561 tcgacgtgcg ctcgcggatc gacatctaca accagctgcg cgacggcgcc cgccgcggcc
    10621 tcgccgtggt catggtctcc tccgacgcct ccgagctggc cggcctctgc gatcgcgtcg
    10681 tggtgatgtc gcgcggcaag ctggtcgccg agctggcgac cgaggaggcg accgaggagt
    10741 cgatcgtcaa ctccttcact gcgctcggcc acggggcagg ggaggagacc ctggccgcgg
    10801 tcgccgccgg cgaggagtcc gccccggccg ccggcgagcc cgccgccccg cgcctctccg
    10861 cctgggagcg cgtccgcagc gtcgtccgcc gcaacgagaa cggcccgcga ctcggcctcc
    10921 tggcgctgct cctggtcctg atcggcgcct atgcccagag cgagaacgac gtcttcctct
    10981 cgcacgccaa cgtctacaac ctgcttttcc tggcggtgcc gctggcggcg atcgccgcgg
    11041 ccgagttcgc ggtcctctac gtcggcgaga tcgacgtctc ggtcggcgcc gtgatggcgc
    11101 tgatgatcgt gctcatgtcg gagttcgtgc agagcggctc gttcgtcgtc gccctgctgc
    11161 tctcgctgct gatcgcgctg atcatcggcg tcgtcgtcgg cctcggcaac gcggctctgg
    11221 tcgagggggc gcggatctcg ccgatcatcg ccacgatcgg gacgctcggg atcatcggcg
    11281 gcgtcgggct gatgatccgg ccgaccgccg gcggctcgat cagcaccgac ctgctgaacg
    11341 cgctggccga ggaagtctgg gtcctgccct ggagcttcgt cgtgctcgtc gtcctcttcg
    11401 tgctcggcga ctgggccctg cgttcgaccg gggccgggct gcggctgcgc tcggtcggcc
    11461 tcaacgccgc cttcgcccac cggctcggcg tcaacgcgcg cctcgtccgt accgccgcct
    11521 tcgtcgtcgc ctcgttcttc gccgcctgcg ccggcctcct gctctccgcc cagatcggga
    11581 tcggcgatgc gaccgtcggc cagggctacc tgctgctggc gatcgccgcc ccggtgctcg
    11641 gcggcgccag tctcgccggc ggccacggca ccttcgtcgg ctgcctgctc ggagcctgcc
    11701 tgctggtcac ggtccagaac ctgaccaccc tgctggggat caaggacgcc tacagctacc
    11761 tgcttaccgg cggcctgacg atcctcgccc tgctgctgta cacgagcggc gccggagccg
    11821 cgatccgcaa ttacgtcagg tcgctccgcc tccgcttcgg cggcggggcg cccggagagg
    11881 ccggtgccaa agcatgaccg ccgtcaaggt ccacgacctg tttgcccacc gcgaggatct
    11941 cagccgcgag gagttctttg cctactggcg ctcggcccac gccggcctgg cgacgacctt
    12001 caaacagatc aagcactaca cccaggccca ccgcagcggc gaccaggatc tggtccccgg
    12061 gttcgccgag acctggcccg atggctgcgc cgagacctgg tacgacgacg cctcagaggt
    12121 cgatgcgatg gtcgcggaac cgcgcttcgc cgccgagctg atggaggacg agaagaactt
    12181 catgaacctc gagaatccgc ggccgctgct ggtctcggag ctgtcgctgc tcgacgagaa
    12241 cgggttcgac cccgaggacc gcgggatcaa ggtgctgatc ttcgccgccc gcccgggcgg
    12301 gacctcgcgc gaggagttcc tcgccgcctg gacgagcgac gatgatgcgg cgctcggcca
    12361 cgagctcggc gccacccgcc aggcccttta caccccggtc accaccggcg gcgtgctgat
    12421 ccagaccgac ctcgaggacg gctcccatca ggagggcggc ggctcctacg acgccgtccg
    12481 cgaggtctgg tggaaggatc gcgccgggct cgaggcggcc accgccgagg acaacgagcc
    12541 ctggcggcgg ctgctgcgca aggacgccct cgacacctct cgctcgatca ccctgatcgc
    12601 ccgcgagaac gtcgtcctcc cttagacgca aaccaaacga acgtatggta ggcttttgac
    12661 atgttcgccg ggtcgagcaa cagggtcggg atcgtcgcca gcgcgcagac gcagttcaag
    12721 cgcgcctggg cggatcggca gcacgtcgac ctgatcggcg aggtcgtcaa cgcggccctc
    12781 aaagggtccg ggctgcggat cgaggacgtc gacttcgtga tcgactccgg cagcgacatc
    12841 ctcgacgggc gcagcatctc caactgcggc ttcctcggcg ccatgggtgc ccaccagaag
    12901 gaagagtcgc gggtcgagga ggacgggctc tggtcggcgc tgtacggcgc caccaagatc
    12961 ggcgccggct cggccgaggt cgggctgctg gtcgcctact cgaagccttc ggagtccgac
    13021 gtcgccgcct tctactggtc gcagtgcgag cccttctacc agcgcccggt cggcttcggc
    13081 caggccgccg ccagcggcct ccaggcccag agctacctcg ccgagcacgg cctctcggag
    13141 gagatcttcg ccgacgtcgc cgccgccgag tgggcgaagg cggtcgcgaa cccggccctg
    13201 gagatcggcg acgctccgag cgccgccgag gtccgcgaat cgggcaacgc cgcggcgccg
    13261 ctgcgcgagc tcgagatgtc ccagccggtc gacggcgcgg ttgccgtggt gatcgcctcg
    13321 cagcgggtcg ccgagcggat ctgcccggac cccgtctgga tcaccgggat cggcacggcg
    13381 atggaccaac acgccttctc acgtcgtcag cgttccgcgc tgcccgccgc cgcggttgcc
    13441 gccgagggcg cctacaagcg ggccgggatc gacggccccc agggcctctc gctggccgag
    13501 gtctccgcct cctcctccgc cacccagctg atggtgctgg aggccctcgg gctcgcggag
    13561 gccggcaagg gcgccgccct ctacgaggac ggctcgaagg tcgcgatcaa cccttccggc
    13621 ggtgctctgc ccgccgaccc gatcatggcc accgggctgg ctcgcctcag cgaggcggcg
    13681 ctgcagctca cgggccgcgt cggccatgcc ccggagggcg cgagctcggc ggtcgtccac
    13741 ggcgccggcg gcgtcggcat gcaaaaccac tgcgtcttca ccctggaggt ctgaggtgcc
    13801 acgcgacgtc gcaatcgtcg gaaccggaca gacggtctac gcccgcaggc ggaccgacgt
    13861 cagccaggcg ggcctggtcc gcgaggcggt cgacgccgcg ctcgaggacg ccgagctgac
    13921 cttcgccgac gtcgactcga tcgtcgtcgc cagcggcccg gccctgttcg gctcggtcaa
    13981 ccagcctgag aagtggctgg tcgacgggct cggcggcctc atgaagccgg tggtgcggat
    14041 caccagcggc ggcggcaccg gcctcgccgg ggcgctcgcg gcgatgaacc aggtctccag
    14101 cggcttcgcc gagcgggtcc tcgtcgtcgc ctacgacaag ctctccgagg gcgccctgca
    14161 gtaccagatc tcgaccctct acgacccctt ctgggggcgc gagttcgcgg tcgggatcat
    14221 gggcttcgcc tcggcctact ggcgggcgcg gatcgagacc ctcggccaca ccgaggaggc
    14281 ggccgcgatg gtcggcgtca agaaccaccg caacgccttc ctcaacccct acgcccacgt
    14341 gcgcaaggag atcacggtcg aggacgtgct ggcgagccgg cccctggcct ggccgatcaa
    14401 gctgctcgac gtgccgccga tctccgacgg cgcctgcgcc gtcgtcctcg cctcgcccga
    14461 ggcggccgag aagacgaccg cgaagccggc ctggatccgc ggcatgtcct acttcggcga
    14521 ggccgacaac gcgcccgacc gcttcatgct gcagtcggtg ccgctgcagc tggccgcgca
    14581 gcgcgtctac aagaaagccg ggatcaccaa cccgcgccgc cagttcgacg tcgccgagct
    14641 gcaggagccc tacacctgct tcgagctcag ctactacgaa gggctcggcc tctgcccgac
    14701 cggcgaggcg gcgaagctga tcgagtccgg cgccaccgac atggaaggcg acatcccctg
    14761 taacccctcg ggcggctgca tgggcgccaa cccgatcgga gccacggcgc tgatccggct
    14821 cgccgaggcg gcgctgcagg tgaccgacaa ggccggcgaa cgccagatcg aaggcgcccg
    14881 cacggcgctg gtccaggccg gcggcggctg ggcgaacctg aggggcgtcg ccgtggtcgg
    14941 cgccgagcgg aactaggagg cttgagatgg agagcagcgc ggtggagaag atggcgatgc
    15001 ggggcagcgg gctcggctcg accccgaacg acaacccgac cttcgccggc aagatcgacc
    15061 tgccctacac gctgaccacc ggccgcgccg cggcgacctt tctcgccgag ctggcgaaca
    15121 agaagatcgc cggcagccgc tgctccgagt gcaaggaggt caccgtcccc gcccaggact
    15181 tctgcgccgc ctgcgggacg accctcgagg agctcgtcga ggtctcggcg agcggcgagc
    15241 tggccggctt caccgagacc gccgagggcc tgatcgggct gatccggctc gacggcgccg
    15301 ccaacgagtt cccgcacaga ctgctcgaca ccgagttcgg ctcgctgagc gtcggcgacc
    15361 gtgtccaggt ccgctgggcg gccgagcccg agggttcgat cctcgacatc gagggcttcg
    15421 agccgggcgg cgagcccggg ggagaggtgg tgccgctgga ggccagcgcc gagccggtgc
    15481 tggaacagaa atacgggatg cggctcgatt acgagcacgc ctacggcccc tactacggga
    15541 tgctcttcga cgggctcgct accagccgcc ggatccaggg cagccgctgc cccagctgca
    15601 cccgggtcct ggtcccgccg cgcgagtact gcgacgtctg ttacgtgcgc accggcgagt
    15661 gggtcgacgt cgccgacacc ggcacgatca aagccttctc gatcatccac ctcgagttcg
    15721 tcggccagac ccgcgaaccg ccctacgtct acgccgagat cgttctcgac ggctccgcca
    15781 cgcggctgat tcacaccgtc ggcgggatcg acatcgagga ggccaaggag cggctgcgga
    15841 tcggcaccaa ggtgcgcgcg gtctggaaag agggcggcga gccggtcggc acgctcgagg
    15901 acattgacca cttcgagccg ctcttcgagg agtgaggatg gcggggttcg agatggtcga
    15961 gctcgaggtt gccgacggcc tcgcgaccgt caccctcaac cgtcccgacg cgctcaacgc
    16021 gctcaacgtc cagcttaaag acgagctggc cgaggtctgg cgccggctcg gccccgaccc
    16081 cgaggtccgc gcggtgctgc tgaccggcgc cggccgcgcc ttctcgaccg gcggcgacat
    16141 caagcagatg gaccccgacc gcaccccgga ggcgacccgg gtgatgatcg ccaagctcgg
    16201 gcgcgaggtg gtgatcccgc tggcccaact ggagaagccg gtcgtggccg cggtcaacgg
    16261 ccacgcgcac gggctcggcc tcgcgctggc gctcgcctgc gatgtgatct acgccgccga
    16321 gagcgccgtg ctctccgccg cctttatccg catcggcctc tccccggacg gctgctgctc
    16381 ctacctgctg acccgcaccg tcggcatcct cgccgccaag gagctgatgt tcagcggtcg
    16441 ccggatcgac gggatcgagg cgcagcagct cgggctggtc acccaggtcg tcgccggcgc
    16501 ggagcttctg ggcaccgccc gcgaggcggc actcggcttc gccgcggcgc cgacgatcgc
    16561 cctcgcccga accaagcgac tgctgaacga ggcggcccag cacacgatcg aggagacggc
    16621 cgagctcgag gcccttgcgc aggccgtcgc gcgcggcgcc gacgaccacg cggagggcgt
    16681 cgccgccttc gccgagaagc gcccggccac cttcacgggg agctgacaga tgccggccta
    16741 cgacgagctg ccagtgatcg agaagctcgg catgccgcac gcctgggacg cgctcgatca
    16801 cgacctcggg acgctcgacc gcggcacccc cgagcaggtc accgccgccg ccgagctggt
    16861 ccgtgagggc accgtgctga cgctcaacct gccgctgaac gagccggatc cgccgctatt
    16921 tgggcgcgac ccgatccgcc aggagatctt cgccacggat cgcaacacgc tcgacgaccg
    16981 cttcgacgcc ttctacccgc agggttcgac ccagtgggat ggcctacgcc acgtgcgcgc
    17041 ccgcgagttc ggtttcttcg gcggcctcac cgacgagttc gagcccggcg acgagcggct
    17101 ctcgataaca cactgggcgc ggcgcgggat cgtcggccgc ggcgtcctcc tcgacctccc
    17161 ggcccaccgc gagcgccgcg gcgagccctg ggacccgttc gccgccgagc cgattcaagc
    17221 cgaggagttg gaggcgatcg tcgccggcca gggcgtcgag ctgaagcccg gcgacgtgct
    17281 ctgcctgcgc accggctgga tcgacgccta ccgcgaactc gaccgggccg gccgcgagcg
    17341 tgctgccgcc gaggtcgcga tcagcggcct cgccggctcg gaggcggtcg cccgcttcct
    17401 ctgggacagc ggcgcgatcg cgatcgctgc cgacaacccg acgctggagg tcgcccccgg
    17461 cgaccccgcg gtcggctccc tgcaccgtcg tctgatcccg ctgctggggt tcgcggtcgg
    17521 cgagctgctc gacctcggcg tcctcgctgg ccactgcgct ggcgactcgc gctgggactt
    17581 catgttcgtg tccgtgcccc tcaacctgcc gggcggcgtg ggctcgccgg cgaatgcgat
    17641 ggcgatcaga tgaacacctc ggtgaagacc cttcccagca gcgtcctcgg ccccgcgatc
    17701 tcggtggcgc cggaggacca ccccttccgc caggagttcc gcgcctggct ggccgacaac
    17761 gtcccgggcg tcccggagcc gctcgaccag gacgagaagt tccagatgcg ccgcgcctgg
    17821 cagcgcaccc tcttcgaggg cggctgggcc ggaccggcct gggccgagga gtacggcggc
    17881 cgcggcgccg ggcccctgca ccagttcatg tactacgagg agctggccct ggcacgggcg
    17941 ccgttcatcg ccaacacgcc cgggatctcg ctgctcggcc cgaccctgat cgaggtcggc
    18001 tccgacgagc tgaagagccg cttcctgccg gtgatgctct cctccgagga gatcttctcc
    18061 cagggattct ccgagcccgg cgccggctcc gacctggccg cgatccgggc tcgcgccaaa
    18121 cgcgacggcg aggagtgggt gatcaacggc cagaagatct ggaccacctg ggcccagtac
    18181 tcggactact gcttcgttct ctgccgcacc gaccccgagt ccgagcgcca ccgcggtctc
    18241 agcctgctga tctgcccggt cgaccagccc ggggtgacgg tgcgcgagat cgaacagatc
    18301 agcggcgacc ccgagttcgc cgaggtcttt ttcgatgacg cccgctgccc cgcctcctgg
    18361 gtcgtcggcg aggtcgacga cggctggtcg acggcgatga tcctctttca gttcgagcgc
    18421 ggtgaccagg gcttcaccga ccactcccgg atcctcgtct ccctccacga cctcgccgcc
    18481 gagctgcgcg aggcgagcca gcgcgagtcg atctcgcggc cctcggtcga ggcggcccgc
    18541 cgccgcctcg ccgacctctg ggtgcgcgcc cagcagctgc gccggatcaa cctgcggatc
    18601 gcggtgcgcc aggaggccgg cgaatcgatc gggatggaag gctcggtggt gaacatcttc
    18661 tggggcgagc tggagcgcga cctcggcgag ctgaccgccg ccgtgcacgg gccgcgcggg
    18721 ctcctgctcg gcaccgaccc gggtcacgag gtgctggcgg cgcgggccgg gacgatctac
    18781 tccgggacca gcgagatcca gcgcaacatc atcgccgagc ggatgctcgg gctgccgcgg
    18841 tgacgatgag cattccagcg accgacacgc cgctgcgccg cagcctcgtc ttcgccaccg
    18901 acgagctcga gccgctgccc gagctggccc gccgctgcga ggccggcggc ctcgaccgcg
    18961 tctggacgac cgagtacgtc ggccgcgacg cgatcgcccg cgccctcgcc gtcgccctcg
    19021 ccaccgagcg gatcgaggtc gcgaccggga tcacctacgc gttcacccgg gtgccgctgg
    19081 cgatggcggc tcttgccgcc gacgtgcagc ggctctcggg tgaacgcttc gcgctcggaa
    19141 tcagctccgg cacccgcggc gtgcgccgct ggtacggggc cgagttcgaa ccgccggcac
    19201 cggcgctggc ggagtacgcc ggggcgctgc gcgaggcttg gggcaagatc cccgagctgc
    19261 agcagccgcc caaggtctac ggcgcggtgc tgaacccgat catgacccgc cacgtcgccc
    19321 gcagttgcga cggcgccctc ctgcacgcgc tggcgctgag ccgcacccac ctgcgcgagc
    19381 ggctgctgcc ggcactggcc aagggcctcg agcagcgtga ggatccacgg ccgctggaga
    19441 tcgccgcctg gtgcatcacc tcgatcgacg aggacgagga gcaggcccgc gagaacgccc
    19501 gtcgccagct cgccttctac ctctcgactc cgagctaccg cacggtcgcc gaggggacct
    19561 cgtgggagca ggtcgccggc gaggttcgcg aggcctacga ggcctccgac cgcaaggccg
    19621 cctggggcga tctctcccac ctgatccccg accacgtggt cgacgagctg acgatcagcg
    19681 gcaccccggc gagcgcgccg gagcgggccg cagcgatgga agcggagctg gccgccgccg
    19741 gggtcaccga gcttgtcttc caaaccgccg gcgccgacgt tcccgccgcc gagctgatct
    19801 ccagctgcga acagattgcc gacgtgctcg gcgccgactc gaaggtgagc aaatgaccac
    19861 gaccgttacg ctgagccccg aacaggacga gatccgcggg gtcgcgcgga gcttcctcga
    19921 ggcgcgatat ccctccgacc gggtccgcga gctgatgaag acgccggcgg gcttcgagca
    19981 gtccgactgg gacgagatcg ccgagctcgg ctggactggg atcgccgtac ccgaggggca
    20041 aggaggagcg ggctacagtg acgtcgagcg ctgcctgctg ctcgaggaga tgggccgtgt
    20101 gctctttccc tgtccctttc tctcctcggc ggtcctagcc gccgacgccc tcgccctggc
    20161 gggcgagggc ggtgcggcgg cagagctgct gcccggggtg gtcgacggct cgacccgggc
    20221 gacgctggtc gccggcggcg acctcggggc cggccagggc tctgcgctgc gcgccgtcgc
    20281 tcgcggcgag gagcacgagc tgagtggcaa cggcggcctc accctcgacg gccacacggg
    20341 cgagctgctg atcgtcgtcg ccgcgctcga gggaggtgag ctcgccctgt tcgcggtcga
    20401 cccgggcgcc gagggcgtca tccgcaaccc cgtcgagacg atcgacgaga cccgcaagac
    20461 ggccgtcgtc gagttcgagc gcacccccgc ccaccgtctt gacggcggtg acggcggcga
    20521 ggggatcgcc ctcgcgctcg accgcggcgc cgtcgctctc gcggccgaga tggccggcgg
    20581 cgcccgcaag gcgatcgaga tgaccgtcgc ctacatgcac gagcgcgagc agttcggcgg
    20641 gccgatcggt cgcttccagg cgctcaagca ccggctcgcc gacctccacg tgcggatcga
    20701 cgtggcccgc cagagcgtct acgctgcggc ggatgcgctc gccgcggggg aaagcgagca
    20761 cgtgccgatg ctggctgctg ctgccaagta cgcggcgtct atcggttacg tgaccgccac
    20821 cgccgaagcg atccagctac acggtggcat cggcttcacc gaggagcacg acatcggcct
    20881 ctactacaag cgcgccctgg tcagcgctga gacgctcgga gcgccggccg accaggtcga
    20941 gcggatcgca ctcggcctcg atgtctgagc ccgccgcccc gatgccggag ccggacgtct
    21001 tcgaccggct ctactttgag gacatggagg tcggccgcgg ctgggtctcg ccgcggcgta
    21061 cgatcggcga gggcgacgtc agcgccttcg ccggcctctc cggcgacttc aatccactcc
    21121 acgtcgatgc ggtgttcgcc gccgagaccg ccttcggcaa gcggctcgtc cacggtgccc
    21181 tggtcttcgc gatcgccacc gggctgcgcc agcagatgcc gatctttcgc ggctcgatgc
    21241 g
//
LOCUS       DASRIG010000464         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1266325, whole genome shotgun sequence.
ACCESSION   DASRIG010000464 DASRIG010000000
VERSION     DASRIG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266325"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VFB52_12510"
     CDS             <1..1461
                     /locus_tag="VFB52_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO07202.1"
                     /translation="RGVYKVVGTVRKDGDGLTFSNAGTDPQAGILNATYGTWRGSVAT
                     ALNSTLAHDQLFAIGGAMRHVHFDPTPHTLNCASYPSSVSCGGLIGVHQTLLMTHLTL
                     GKMMSASPTLSRDVLGQGAACMFPVISIAGLDANGREFGTGILDAMMGGLGAFSFRDG
                     VDTGGIYFIPKGRAANVEEAEDMFPVLYLARREKRDSGGAGKFRGGNSFEALLIPHGT
                     EEIMQTTASGAVGVSAALGLSGGLPGCPNFYEIVRGTGILEQLAAGEVVTEADQISGE
                     HEELPGKSSGSCQKPDDVYAMHATAGAGFGDPLRRDPVAVLADVSLGQVSEAAARELY
                     GVALNGEQVDAEATERVRAELAKARLEAAGSGNGAGFEHGLDQVRALGAHLGVVRADG
                     GFRFACLECGHDLGAADGNYKGSCAHADVPLEQTNLLAIDASKWVDADVVLREFYCPG
                     CATMLETEMTVREAPPQRDLALRDPAQLFDLATEEV"
     gene            1464..2582
                     /locus_tag="VFB52_12515"
     CDS             1464..2582
                     /locus_tag="VFB52_12515"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZO07203.1"
                     /translation="MAGASGRAAVVREYRAPLRIERFPVPEPAPGALVVGIELSTICG
                     SDVHGWQGAYEGILPTELPLILGHEGVGRVTAIGEGAEVDSLGTPLTLGDRVVWASEP
                     CRHCRMCTVEKKPELCPNRQLGMLTSAARPPHFFGMFAEYGYVFPKAGRLRVPDELPS
                     DWASAATCAGRTAANAVERAGRLDYTDRVVVQGAGPVGLFCLAMALARSPRQTIVIGA
                     PEERLAIAREWGADAAVSIEEHDAASRLEAVMELTEGEGADVIFEASGGPGAVAEGLR
                     MAAREGRYVLIGATGAGTQEIEPHLIINRSLSVRGSFSGDIDSFHKAMLFMLAHRDRF
                     DWNAVIGNRYPLEKLTEALEEMSAMRDIKPVIVPGGDD"
     gene            2584..>3872
                     /locus_tag="VFB52_12520"
     CDS             2584..>3872
                     /locus_tag="VFB52_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO07204.1"
                     /translation="MTRHAPIAIGQMIADQARFGERTALVAGGRRLTYAELLERAERL
                     AAGLRELGVEPGQRFAFLLPNGFEIVTAYYGCALSGIVGVPLATRLTDEDLVHQLADS
                     GAVAVLFDEQFASRVETVHDRAPGVRHWIGFAAVPGAISPEGVLAGAAPESSLGGAVV
                     DRLDDPFCVMFTGGTTGVSKAAIQTQRAWGACIADTVEQLGIEAGDRHAVVLPMTHAA
                     WFTAAAHLAVGAETHLLERWDPVALLELTEAVRLTKLHMMPTLLGDLLTALGERPTDV
                     SSVGLLSLAGSPIPLDLYRRARAAFGEVIANIYGLTEAAGPVTYLVPAELNEDRLRSG
                     GRTGRYVEIEIADEGADEEGHTVGEVRLRGPQLTPGYLNRSEETAAAYEGEWFCTGDI
                     GYLDEDGFLFIVDRKKDMVKTGGFNVYPKEIEEVLYE"
BASE COUNT          546 a         1403 c         1366 g          557 t
ORIGIN      
        1 cgcggcgttt acaaggtggt cggcaccgtg cgcaaggacg gcgacgggct gaccttctcc
       61 aacgccggca ccgacccgca ggcgggcatc ctcaacgcca cctatgggac ctggcgcggc
      121 tcggtcgcga ccgcgctcaa ctcgaccctc gcccacgacc agctgttcgc gatcggcggg
      181 gcgatgcgcc atgtccactt cgacccgacc ccccacacgc tgaactgcgc ctcttatccc
      241 agctccgtca gctgcggcgg cctgatcggc gtccaccaga cgctgctgat gacccacctc
      301 accttgggca agatgatgtc ggcctcgccg accctttccc gcgacgtgct cggccagggc
      361 gccgcctgca tgttcccggt gatctcgatc gccggtctcg acgccaacgg ccgcgagttc
      421 ggcaccggga tcctcgacgc gatgatgggc ggcctcggcg ccttcagctt ccgcgacggt
      481 gtcgacaccg gcggcatcta cttcatcccc aagggacgcg ccgccaacgt cgaggaggcc
      541 gaggacatgt tcccggtcct ctacctggcg cggcgcgaga aacgcgactc gggcggggcc
      601 ggcaagttcc gcggcggcaa cagcttcgag gcgctgctga tcccgcacgg gaccgaggag
      661 atcatgcaga ccaccgcgtc cggcgcggtc ggcgtcagcg ctgcgctggg cctctccggc
      721 gggctccccg gctgcccgaa cttctacgag atcgtgcgcg gcaccgggat cctcgagcag
      781 ctcgccgccg gcgaggtggt gaccgaggcc gaccagatct ccggtgagca cgaggagttg
      841 cccggcaagt cctcgggctc ttgtcagaag cccgacgacg tgtacgcgat gcacgccacc
      901 gccggcgccg ggttcggcga cccgctgcgc cgcgaccctg tggccgtgct cgcggacgtc
      961 agcctcggcc aggtcagcga ggcggccgcc cgtgagctct acggggttgc gctgaacggc
     1021 gagcaggtcg acgccgaggc caccgagcgg gtccgcgccg agctggccaa ggcccggctc
     1081 gaggcggccg gctccggaaa cggcgccggt ttcgagcacg gcctcgacca ggtgcgggcg
     1141 ctcggcgccc accttggcgt ggttcgagcc gacggcggct tccgcttcgc ctgcctcgag
     1201 tgcggccacg acctcggagc cgccgacggc aattacaagg ggtcctgcgc gcacgccgac
     1261 gtgccgctcg agcagaccaa cttgctggca atcgacgcca gcaagtgggt cgatgccgac
     1321 gtcgtcctgc gcgagttcta ctgccctggc tgcgcgacga tgctggagac cgaaatgacg
     1381 gtgcgggagg ccccgccgca gcgcgacctg gcgctgcgcg acccggccca gctattcgac
     1441 ctggcgaccg aggaggtctg agcatggccg gcgccagcgg gcgcgccgcg gtcgtccgcg
     1501 agtatcgggc tccgctgcgg atcgagcggt tcccggtccc cgagcccgcg cccggggcgc
     1561 tggtcgtcgg gatcgagctg agcacgatct gcggtagcga cgtgcacggc tggcagggcg
     1621 cctacgaggg catcctgccg accgagctgc cactaatcct cggacatgag ggcgtcggcc
     1681 gggtgaccgc gatcggcgaa ggggcggagg tcgactcgct cggcacgccg ctgacgctcg
     1741 gcgaccgcgt cgtctgggcc tcggagccct gccgccactg ccgcatgtgc acggtcgaga
     1801 agaaaccgga gctctgcccc aaccgccagc tcgggatgct gacctcggcg gcgcggccgc
     1861 cgcacttctt cgggatgttc gccgagtacg gatacgtctt cccgaaggcg ggccggctgc
     1921 gcgttcccga cgagctgccg agcgattggg cctcggccgc gacctgcgcc gggcgcaccg
     1981 ccgccaatgc ggttgagcgc gccggcaggc tcgactacac cgaccgcgtc gtcgtccaag
     2041 gcgccggccc ggtcgggctc ttctgcctgg cgatggcgct ggcccgctcg ccgcgccaga
     2101 cgatcgtgat cggtgccccc gaggagcggc tggcgatcgc ccgcgagtgg ggggccgacg
     2161 cggccgtctc gatcgaggag cacgacgccg cgagccggct cgaagcggtg atggagctga
     2221 ccgagggcga gggtgccgac gtgatcttcg aggcctccgg ggggccgggc gcggtcgccg
     2281 aggggctgcg gatggcggca cgcgagggcc gctacgttct gatcggcgcc accggcgccg
     2341 gtacccagga gatcgagccg cacctgatca tcaaccgctc gctctccgtg cgcggctcgt
     2401 tcagcggcga catcgactcg ttccacaagg cgatgctgtt catgctcgcg caccgcgacc
     2461 gcttcgactg gaacgcggtg atcggcaacc gctacccgct cgagaagctc accgaggcgc
     2521 tcgaggagat gtcggcgatg cgcgacatca agccagtgat cgtccccggc ggagatgact
     2581 gagatgaccc gacatgcgcc gatcgcgatc ggccagatga tcgccgacca ggcccggttc
     2641 ggcgagcgca cggcgctggt cgccggcggc cggcggctca cctacgcgga gctcctggag
     2701 cgcgccgagc ggctcgccgc cggcctgcgg gagctcgggg tcgagcctgg ccagcgcttt
     2761 gccttcctgc tcccgaacgg tttcgagatc gttaccgcct actacggctg cgcgctctcg
     2821 gggatcgtcg gtgtgccgct ggcgacccgg ctcaccgacg aggacctggt ccaccagctg
     2881 gccgactcgg gcgcggtcgc ggtgctcttt gacgagcagt tcgcctcccg cgtcgagaca
     2941 gtccacgacc gcgcccccgg cgtgcggcat tggatcggct tcgccgccgt cccgggcgcg
     3001 atttcccccg agggcgtgct ggccggtgcc gctccggagt cgtcgctggg cggtgcggtc
     3061 gtggaccgcc tcgacgaccc cttctgcgtg atgttcaccg gcggcacgac cggcgtctcg
     3121 aaggcggcga tccagaccca gcgggcctgg ggcgcctgca tcgccgacac cgtcgagcag
     3181 ctcgggatcg aggcgggcga ccgccacgcg gtggtcctgc cgatgaccca cgccgcctgg
     3241 tttaccgccg ccgcccacct cgcggtcggc gccgaaacgc acctgctgga gcgctgggac
     3301 ccggtcgcgc tgctggagtt gaccgaggcc gtgcggctga ccaagctgca catgatgccg
     3361 accctgctcg gcgacctgct gacggcgctg ggcgagcggc cgaccgacgt ctccagcgtt
     3421 ggcctgctca gcctcgccgg ctcgccgatc ccgctcgacc tctaccgccg cgcccgcgcc
     3481 gcctttggcg aggtgatcgc caacatctac gggctgaccg aggccgccgg cccagtcacc
     3541 tacctggtgc cggccgagct caacgaggat cgcctgcgct ccggcggccg caccggccgc
     3601 tacgtcgaga tcgagatcgc cgacgagggc gccgacgagg agggccacac ggtcggcgag
     3661 gtgcggctgc gcggcccgca gttgacccct ggctacctaa accgctccga ggaaaccgcg
     3721 gccgcctacg agggcgagtg gttttgcacc ggcgacatcg gctacctcga cgaggacggc
     3781 ttcctcttca tcgtcgaccg caagaaggac atggtgaaga cgggtggctt caacgtctac
     3841 ccgaaggaga tcgaggaggt gctctacgag ca
//
LOCUS       DASRIG010000465         7263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_225179, whole genome shotgun sequence.
ACCESSION   DASRIG010000465 DASRIG010000000
VERSION     DASRIG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225179"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFB52_12525"
     CDS             <1..401
                     /locus_tag="VFB52_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZO07205.1"
                     /translation="PDPGPVPPTPEPGPPVPPEPAPVPGPPPGPPPGPTPAPEPPVPT
                     PDPGPPPTPLPTQPEPPAPAAASAGGAQINLNSVTFEQLRAQDLSVTQATRLLAHRER
                     MGGFQSVDDLDGVDGFPAEVLAQLKGRATV"
     gene            complement(666..2048)
                     /locus_tag="VFB52_12530"
     CDS             complement(666..2048)
                     /locus_tag="VFB52_12530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO07206.1"
                     /translation="MSEQNFDAIVLGAGPSGEVCAGRLADAGWSVAIVERDLVGGECS
                     YYACMPSKALLRPADVLAEAQRVPGVPADDSSSLDPQAVLDRRDEVIHDRDDSGQLPW
                     LEERGIELFRGAGRFTGERRVEVGDDVLVAAKAVVVATGSGAAMPPIPGLDEVQTWNN
                     RDATTAKQVPASMVVLGGGPVGSELSQAWSSLGTKVTLIEGNGHLLGRDEPFAGEEVA
                     AALRERFGVDVRTGARAAKVSADGPGVVVELEGGDSVEGEQLLVAIGRVPHTADLGLD
                     QIGVEPGEHGFLATDDHLRAGGSEWLYAIGDANGRALFTHIGKYQAWVATENLLGRPV
                     EALAEGLGSPRVTFTDPQVAAVGKTLQQAEDAGIDARSVDVPTDGTPGASFQGKNTGG
                     ISRIVVDQAKGTIVGATFTGFETADFLHAATIAVVAEVPLAKLRHAVAAYPSRSEIWL
                     KLLEAYETDG"
     gene            2095..2458
                     /gene="ssrA"
                     /locus_tag="VFB52_12535"
     tmRNA           2095..2458
                     /gene="ssrA"
                     /locus_tag="VFB52_12535"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2497..2760)
                     /locus_tag="VFB52_12540"
     CDS             complement(2497..2760)
                     /locus_tag="VFB52_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO07207.1"
                     /translation="MTSLPPSPAPPPDQPDPALGPVVRGLRKELGLSATELADQAGLE
                     TTTVEQIEAGLVDPPWAAVEAIARSLGVSIESIASAAAEQRPD"
     gene            3034..3300
                     /locus_tag="VFB52_12545"
     CDS             3034..3300
                     /locus_tag="VFB52_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO07208.1"
                     /translation="MPRREDPQFGLGHAIRKLREERELTQESLAHRSDLHPTWISHIE
                     SGRINPTWGNVRRMARGLEVPLCTLAALAEELQRKHDDPECAKN"
     gene            3469..4011
                     /locus_tag="VFB52_12550"
     CDS             3469..4011
                     /locus_tag="VFB52_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZO07209.1"
                     /translation="MDLYVAFLRGMNLGNRRIKNPELAAEFEQLGFVDVATFRASGNV
                     VFAVDGREQRAALSERIETGLLEGLGYDVPVYLRSAAEVTAIAAQAPFSAKEVAASKG
                     KLQVTMLRAKPSAAARKKALAQSSDEDLLAIDGSELYWLPSGGISESDLDLKAIEVAL
                     GPGTQRTMGTIEQIASKYCG"
     gene            complement(4008..4949)
                     /locus_tag="VFB52_12555"
     CDS             complement(4008..4949)
                     /locus_tag="VFB52_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07210.1"
                     /translation="MGGPLRHLGELAKARATRRLSERADGEMLGELEARPLQLPAGLE
                     VEWLGVSGYRLSYEGQTIFIDPYLSRVPFRDLVLRRQALPDPRAVDRFAGKPGETAGV
                     LVGHTHFDHAVDAPAIARRFGCKAYGSASLVNLMGLHGLAEQTVEVEPYRTYELGPFE
                     VSFTPSVHSKLLLGLAVPYDGELSCEHLDGLCPSAYRCGQVWGIAIAVAGVRFYHQGS
                     ANLIDDALREREVDYFLAGVAGRSFTDDYWRRVLTKLQPRAVVPTHYDNFFRPLGQRL
                     EFVGNVQLAALPDEIGAVSREIEIAALPRTDLGDAER"
     gene            4984..6876
                     /locus_tag="VFB52_12560"
     CDS             4984..6876
                     /locus_tag="VFB52_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZO07211.1"
                     /translation="MAIATATQVDVHVGGKLLFEEVSFKLEPGDRMTLSGRNGAGKST
                     LLRVLAGQLAPDAGTVTLHKGARVALHDQRPPRDSESSLGEYVYSGRADLIETEAELA
                     RLEERMSGGDAGEETLAAYAAAQQRLEAGGGYRWRDRVLEVLRGLGFTPEHTERRLST
                     FSGGELTRASLARALASRPDLLLLDEPTNHLDIPSLEWLERYLVDLDAAVVLVAHDRW
                     FLEAVGTSVLELEAGRGRFFAGPWHAWRTEQAARQIALGKAIERQQAEIERMERFVER
                     FRYKATKARQAQSRVKQIDKKKKSGPAAERRDTRRLRFSFKSPERPGRVVLRLVAGKI
                     EVPGRTLLDGAELDLEREEHVVLVGANGSGKTTLIETLAGQRPPAEGSVREGHNVQLG
                     YLSQHADTAAGTGTVLDAAQRETGLTGPKARALLGGFLFSGNEVEKQLSDISGGEQRR
                     LSLAILVASGANVLILDEPTNHLDIESREALEDALTEYEGAVILISHDRALLEAVGSR
                     TIVLEEGKLTSHPSGWAEYQRRRDEAEAASTAAAKSANGKGGNKYSATKPAQKAAKKA
                     ARLEQRIEQAEAELREIEDELADPAAWSSPGGVERANERHAKAKQAIEELYAEWAQAQ
                     EAAEVT"
     gene            complement(6869..>7263)
                     /locus_tag="VFB52_12565"
     CDS             complement(6869..>7263)
                     /locus_tag="VFB52_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO07212.1"
                     /translation="LRSRPVIIDVARKLTAPATIGGYELPAGTFVLPAIAALHQREDI
                     FPAPQEFRPERFLDGKPDTYAWIPFGGGVRRCIGAAFAEYEMRTILREIVERAELTPV
                     DPKPERVRVRNITLAPAKGARVICAEVR"
BASE COUNT         1154 a         2550 c         2534 g         1025 t
ORIGIN      
        1 tgcccgatcc aggtccggtg ccgccgaccc ccgagcccgg cccgccggtg ccgcccgagc
       61 ccgcccctgt tccagggccg ccgcccgggc ccccgccggg accgactccg gcgccggagc
      121 cgcccgtccc gacgccagac cccggcccgc cgccgacgcc gctgccgacg cagccggagc
      181 cgcccgcgcc cgccgcggcc agcgccgggg gtgcgcagat caacctcaac tcggtcacgt
      241 tcgagcaact gcgagcccaa gacctctcgg tcacccaggc gacccgcctg ctcgcccacc
      301 gcgagcgcat gggcggcttc cagtcggtcg acgacctcga cggcgtcgac ggtttccccg
      361 ccgaggtcct cgcccagctc aagggccgcg cgacggtcta gggcagtacg ccgatccccc
      421 acggttcctt tccggacata tcgaccggaa aggaaccacg cgacccaaca atcacggctc
      481 cgaggccccg ccacggcccg aaagccagac ggaacccccg ggatggcccg tgtggagggg
      541 tcgggtgact cccggaacgt cttgtacttt tttgctgcag cggatcgagg cgtaaagccg
      601 ccctcgatcc acaaaaaagt taagtgcagg gagtcacccg acccctcccc gcatgcgatc
      661 ccggattacc cgtccgtttc gtaggcctcg agcagcttca gccagatctc gctgcgcgac
      721 gggtatgccg cgacggcgtg gcgcagcttg gccagcggga cctcggcgac gaccgcgatc
      781 gtcgcggcgt ggaggaagtc ggcggtctcg aagccggtga aggtggcgcc gacgatcgtg
      841 cctttggcct ggtcgacgac gattctggag atcccaccgg tgttcttgcc ctggaagctg
      901 gcgcctggcg tcccgtcggt ggggacgtcg acggagcggg cgtcgatgcc ggcgtcttcg
      961 gcctgctgca gggtcttgcc gaccgcggcg acctgggggt cggtgaaggt gacgcggggg
     1021 gagccgaggc cctcggcgag tgcctccacc gggcggccga gcaggttctc ggtggcgacc
     1081 caggcctggt acttgccgat gtgggtgaag agggcgcggc cgttggcgtc gccgatcgcg
     1141 tagagccact cgctgccgcc agcccgcagg tggtcgtcgg tggcgaggaa gccgtgctcg
     1201 ccgggctcga cgccgatctg gtcgaggccg aggtcggcgg tgtgggggac gcggccgatc
     1261 gcgaccagga gctgctcgcc ctcgacactg tcgccgccct ccagctcgac gacaacgccg
     1321 ggtccgtcgg cgctgacctt ggcggcacgc gccccggtgc ggacgtcgac gccgaagcgc
     1381 tcgcgcagcg ccgccgccac ttcttcgccg gcgaacggct cgtcgcgccc gagcaggtgc
     1441 ccgttgccct cgatcagcgt caccttggtc cccagcgagg accaggcctg agacagctcg
     1501 ctgccgaccg ggccgccgcc gagcacgacc atgctcgccg gcacctgctt ggcggtggtg
     1561 gcgtcccggt tgttccaggt ttgcacctcg tcgaggccgg ggatcggcgg catcgccgcg
     1621 cctgagccgg tcgcgaccac caccgctttc gcggcaacca ggacgtcgtc gccaacctcg
     1681 acccggcgct cgccggtgaa tcggcccgcc ccgcggaaca gctcgatccc gcgttcttcc
     1741 agccagggca gctgacccga gtcgtcgcgg tcgtgaatca cctcgtcacg gcggtcgagg
     1801 accgcctgcg ggtcgagcga gctggagtcg tcggcgggca cgccgggcac ccgctgggcc
     1861 tcggcgagca cgtcggcggg tcgcagcagc gccttcgagg gcatgcacgc gtagtaggag
     1921 cattcgccgc cgaccaggtc gcgctcgacg atcgccaccg accagccggc gtcggcgaga
     1981 cggccggcgc agacctcgcc cgaggggccc gctccgagca cgatcgcgtc gaagttctgc
     2041 tcgctcacac gtgcaccatc cccgacaacg tacaatttca agcgaagttc gcacgggggc
     2101 gacaggcttc gacgtggtcg attcgtgcga aaggttgcag gtcgaggttg tcggaggcct
     2161 cgttaaaccc cgacacaaac caatacgtgc ggaccatgag ttcgccctcg ccgcttagtt
     2221 agaagctaag cgctgtgaga gtcggtcctc cctcgcccgt ggggaggggc accggcttca
     2281 gaaaacgggc ttacccgggc ggatcgcccc gttcaccagg gaaaatcaaa gagggctttg
     2341 tctcccgtag tgcccgctgg cgcggacccc gggagccgag aatcaagcgc cagctaaacc
     2401 tgtagacacc ttcgcgctgc gtccgcggat ccgagttcga atctcggcgc ctccactatt
     2461 tggtcaagac cggagccctc cccaaggggt tccggtctag tccggacgct gctcggcggc
     2521 cgcgctggcg atgctctcga ttgagacgcc gaggctgcgc gcgattgctt ccaccgcggc
     2581 ccacggggga tcgacgagcc cggcctcgat ctgctcgacc gtcgtcgtct ccaggcctgc
     2641 ctggtcggca agctcggtcg ccgacagccc cagctccttg cgcagccccc ggacaactgg
     2701 cccgagagcg ggatcgggct gatccggcgg cggtgcgggg gaggggggca ggctcgtcat
     2761 cggcacccga gatcaaagcg atccaggcgg attcgctcgc tcggactttt tcgcgctcgt
     2821 tgcatcaatt ttggccttga atcgagccgg tggatatacg aacatatgtt cgtgtttgct
     2881 tccgagcaga gagaccggcc ccctcgaacg ggtctttctg cctggaaacc cggcgaatcc
     2941 tcgcgtacca gaatgcgggc tgcgcgggaa tctatacggg acttgagttc acctctatgg
     3001 aattgggggc cggaggcgca ttccctccca tagatgccac gcagggaaga tcctcagttc
     3061 ggtctcgggc acgccatccg caaactgcgg gaggagcgtg agttgaccca ggaatcgctc
     3121 gcgcaccggt ctgacctgca cccgacctgg atctcgcata tcgagtcggg gcggatcaac
     3181 ccgacctggg gcaacgtccg caggatggcg agggggctcg aggtcccgct ctgcaccctc
     3241 gctgcgctcg cagaggaact gcagcggaaa cacgacgacc cagagtgcgc aaaaaactga
     3301 ggcgtccaat cagggaactt gtggtcaact ctatactcgc gccagtaatg ggagccacct
     3361 cggccaggat cccgctatgg cagggagggg ccggcatgta ttggccgatg atgccggtcc
     3421 cttctgaagc cagccggcgg cgcgacccgg cccactaggg tgagtcgcgt ggatctttac
     3481 gtcgcgttcc tgcgtgggat gaacctcggg aaccgccgga tcaagaaccc ggaactggcg
     3541 gcggagttcg agcagctcgg gttcgtcgac gtggcgacgt ttcgcgccag cggcaacgtg
     3601 gtcttcgctg tcgacgggcg cgagcagcgg gcggcgctga gcgagcgaat cgaaaccggc
     3661 ctgctcgagg ggctcggcta cgacgtgccg gtctacctgc gcagcgccgc cgaggtcacc
     3721 gcgatcgccg cccaggcgcc gttctcggcc aaggaagtcg ccgcctcgaa gggcaagctg
     3781 caggtgacga tgctgcgcgc gaagccgagc gcggcggctc gcaagaaagc gttggcgcag
     3841 tcaagcgacg aggaccttct cgcgatcgac ggcagcgagc tctactggct gccaagcggc
     3901 ggcatctccg aatccgacct cgacctgaag gcgatcgagg tggcgctcgg tcccggcacc
     3961 cagcggacga tgggcacgat cgagcagatc gcgtccaagt actgcggcta gcgctcggcg
     4021 tctcccaggt ccgtccgcgg cagcgccgcg atctcgatct cgcggctgac cgcgccgatc
     4081 tcgtcgggca gcgccgcgag ctggacgttg ccgacgaact ccagccgctg gccgagcggg
     4141 cggaagaagt tgtcgtagtg ggtggggacc accgcccgcg gctgcagctt ggtcagcacc
     4201 cgtcgccagt agtcgtcggt gaagctgcgg ccggcgacgc cggcgaggaa gtagtcgacc
     4261 tcgcgctccc gcaacgcatc gtcgatcaga ttggcgctgc cctggtggta aaagcggacg
     4321 ccggcgaccg cgatcgcgat cccccagacc tggccgcagc ggtaggcgct cggacagagc
     4381 ccgtcgaggt gctcgcagct cagttcgccg tcgtagggga ccgcgagccc gagcagcagc
     4441 ttcgagtgca cgctgggcgt gaaggagacc tcgaacggcc ccagctcgta ggtccggtag
     4501 ggctcgacct cgacggtctg ctcggcgagt ccgtgcaggc ccatcaggtt gaccagcgag
     4561 gccgatccgt aggccttgca gccgaagcgc cgcgcgatcg ccggtgcgtc gaccgcgtgg
     4621 tcgaagtggg tgtggccgac caggactccg gccgtttcgc cgggtttccc ggcgaaacgg
     4681 tccaccgccc gagggtcggg caacgcctga cggcgcagga cgaggtcgcg gaagggcacc
     4741 cgcgagaggt aggggtcgat gaagatcgtc tgcccttcgt agctcagccg gtagccggag
     4801 acgcccagcc actcgacctc cagcccggcc ggcagctgca gcggccgcgc ctccagctcg
     4861 cccagcattt cgccgtcggc gcgctcgctc aagcgccgcg tcgcccgcgc tttcgccagc
     4921 tcgccgaggt gtcgcagcgg gcctcccatg cctgaaggct aagcgactca ctacgctcgg
     4981 tcgatggcga tcgccaccgc aacccaggtc gacgtgcacg tcggcggcaa gctgctcttc
     5041 gaagaggtct cgttcaagct cgagccgggc gaccggatga cgctctcggg ccgcaacggg
     5101 gccggcaagt cgaccctgct gcgggtgctc gccggccagc tcgcgcccga cgccggcacg
     5161 gtcaccctgc acaagggagc gcgggtggcg ctgcacgacc agcggccgcc gcgcgactca
     5221 gagagcagcc tcggcgagta cgtctactcg ggccgcgccg acctgatcga gaccgaggcg
     5281 gagctggcgc ggctggagga gcggatgtcg ggcggcgatg ccggtgagga gacgctggcc
     5341 gcctacgccg ccgcccagca gcggctcgaa gccggcgggg gctaccgctg gcgcgaccgc
     5401 gtgctcgaag tcctgcgcgg ccttggcttc acgcccgagc acaccgagcg ccggctctcg
     5461 accttctccg gcggcgagct gacccgcgcc tccctggccc gggcgctggc cagccgcccc
     5521 gacctgctgc tgctcgacga gcccaccaac catctcgaca ttccctcgct ggagtggctg
     5581 gagcgctacc tggtcgacct cgacgcggcc gtcgtcctgg tcgcccacga ccgctggttc
     5641 ctcgaggcgg tcggcacctc ggtgctggag ctggaggcgg gccgcggacg gttcttcgcc
     5701 ggcccctggc acgcctggcg caccgagcag gcggcgcgcc agatcgcgct cggcaaagcg
     5761 atcgagcgcc agcaggcgga gatcgagcgg atggagcgct ttgtcgagcg cttccgctac
     5821 aaggcgacga aggcgcgcca ggcgcagtcg cgggtcaaac agatcgacaa gaaaaagaag
     5881 tccggcccgg ctgccgaacg ccgcgacacc cgccgtctgc gcttctcctt caaatcaccc
     5941 gagcgtcccg gccgcgtcgt tctgcgcctg gtcgccggca agatcgaggt gccgggacgg
     6001 accctgctcg acggcgccga gctcgacctc gaacgtgaag agcacgtcgt cctcgtcggc
     6061 gccaacggca gcggcaagac gaccctgatc gagaccctcg ccggccagcg cccgcctgcc
     6121 gagggcagcg tccgcgaagg acacaacgtg cagctcggct acctctccca gcacgccgac
     6181 accgccgccg ggaccggcac cgtgctcgac gccgcccagc gcgagaccgg gctgaccggg
     6241 ccgaaggcgc gcgccctgct cggcggcttc ctgttctccg gcaacgaggt cgaaaagcag
     6301 ctctccgaca tctccggcgg cgagcagcgg cggctctcgc tggcgatcct ggtcgcctcc
     6361 ggcgccaacg tgctgatcct cgacgagccc accaaccatc tcgacatcga gagccgcgag
     6421 gcgctcgagg acgcgctcac cgagtacgaa ggggcggtga tcctgatctc ccacgaccgt
     6481 gccctcctgg aggcggtcgg cagccgcacg atcgtgctcg aggagggcaa gctgaccagc
     6541 cacccgagcg gctgggccga gtaccagcgg cgccgcgacg aagccgaggc ggcgagcacc
     6601 gcggcggcga agtcggccaa cggcaagggc ggcaacaagt acagcgccac caagcccgcc
     6661 cagaaggcgg cgaagaaggc ggcgcggctg gagcagcgga tcgagcaggc cgaggcggag
     6721 ctgcgcgaga tcgaggacga gctggccgac cccgccgcct ggtcgagtcc gggcggggtc
     6781 gagcgcgcca acgagcgcca cgcaaaggcg aaacaggcga tcgaagagct ctacgccgaa
     6841 tgggcgcagg cccaggaggc cgccgaggtc acctgacctc ggcgcagatc acccgggcac
     6901 cctttgccgg ggccagggtg atgttcctga ctcggacccg ctcgggcttc gggtcgaccg
     6961 gggtgagctc ggcgcgttcg acgatctcgc gcaggatcgt gcgcatctcg tattcggcga
     7021 aggcggcacc gatgcagcgg cgcacgccgc cgccgaaggg gatccaggcg taggtgtcgg
     7081 gcttgccgtc gaggaagcgc tcggggcgga actcctgcgg cgccgggaag atgtcctcgc
     7141 gctggtgcag ggcggcgatc gccggcagca cgaaggtccc ggcgggcagc tcgtagccgc
     7201 cgatcgtcgc cggcgcggtc agcttgcggg cgacgtcaat gatcaccggg cgcgagcgca
     7261 gca
//
LOCUS       DASRIG010000466         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3085722, whole genome shotgun sequence.
ACCESSION   DASRIG010000466 DASRIG010000000
VERSION     DASRIG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3085722"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFB52_12570"
     CDS             <1..430
                     /locus_tag="VFB52_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZO07213.1"
                     /translation="ILVAIDGSPDAEAALRHAAALARDQNARLTLLTVAPNPANTAGV
                     GGAAPPDPLDLHVKALREATDSVPDDTGVTTLLERGDPAHAILATAEKGGHDLIVMGS
                     HGHGRVLRALLGSVSAKVLEKATLPVMILRDGKNKEDIEG"
     gene            complement(725..2245)
                     /locus_tag="VFB52_12575"
     CDS             complement(725..2245)
                     /locus_tag="VFB52_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZO07214.1"
                     /translation="MALFAVALAVVAGCGGGSDDSGSDGGATLDGTGYPGIDNANTRS
                     ADSTIKRDNATSLAVAWKLPLTAESSFGAYASTPVIANGVVYSQDLESNVQAIDLESG
                     EVLWTKRYEEPTGGPNGVAVAEGKVFGATANGAFALDQETGKELWSVELVKNAGGEAI
                     DMAPGTSEGLVYVSTVPAIQASQYPGGGVGTLYALDANSGKEKWSFNTVPPSLWGDKK
                     QNSGGGLWYPPSFDGKGSMYFGTGNPAPFPGTPSDPWGKSRPGPNLYTNSVVKLDAKT
                     GKLDWFYQQTPHDLYDWDFQNPPVLVSAGGKELAIGSGKSGFVTALDAKTGKPVWSTP
                     VGKHNGRDDDGLLAMRGEYSKLKDGLVYPGYLGGVIAPISTNQTAVFVPVVNAPVTVS
                     KGTTIAGAEPEGPGGEVVALDLATGKELWNAEFEGAAYGSTTATNDLVFATSFEGIVH
                     AFDAKTGGEVWSGQLPAGTNSGVSISGDTLIAAAGLAVAEGQKPQIVAYRLGGEGE"
     gene            complement(2304..3668)
                     /locus_tag="VFB52_12580"
     CDS             complement(2304..3668)
                     /locus_tag="VFB52_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidA/IucD/PvdA family monooxygenase"
                     /protein_id="HZO07215.1"
                     /translation="MSAEQKITESDLLIVGAGAKAAAISAKVHVINRLGLGSLSVTIV
                     EKTEPAASWLGRNGMTSGEEPLAIPPIKDVGFPYRSYRQFGETGDAIDAGLLPFTWQR
                     YAVEQGIYAAWVNRESPPIRHRDYGAYLSWVLERATEGIAKFHGRVSSVELTPEGDAW
                     EVEVSERGEPTDPVRHRGRALVLTGPGIHREFAHDPAVADRVFHCDSRRDEFARIPDD
                     ADHEIAIVGGGESALSALVFLRSFRPRVRITVYTPTLPLSRGESFLENRVFADPDDVG
                     WERLDLQTRRDFVKHCDRGVFDMGVLASIAGDRQCSFLTGRAVHVGAEGDKLALEYEG
                     ADGVVAEPYDYVINCTGFDLLAQIRGLFDEGLRAEVERLAGPLWDRPPQTEVPIGRAL
                     ELVGMEPRLHIPGLGGLSQGPGFANLGSLGLLANRVLQPALPGLGQSPSGSGPQQRKS
                     VLID"
     gene            complement(3678..>4019)
                     /locus_tag="VFB52_12585"
     CDS             complement(3678..>4019)
                     /locus_tag="VFB52_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO07216.1"
                     /translation="VVAPCVPLIHNDPKVYPDPDQFLPERFLDGPAPTYSWIPFGGGV
                     RRCLAASYAELEMKRVVRTVLGEVELRPADDSPEGMRKSAISFSPDRSARVIAEPRQA
                     GQPANSTLAAA"
BASE COUNT          580 a         1397 c         1391 g          651 t
ORIGIN      
        1 catcctcgtc gcgatcgacg gctcgcccga cgccgaagcc gccctgcgcc acgccgccgc
       61 cctcgcccgc gaccagaacg cccgcctcac cctgctcacc gtcgccccca acccggccaa
      121 caccgccggc gtcggcggcg ccgccccgcc cgacccgctc gacctgcacg tgaaagcgct
      181 gcgcgaagca accgactccg tccccgacga caccggcgtc accaccctgc tcgagcgcgg
      241 cgaccccgcc cacgcgatcc tcgccaccgc cgagaaaggc ggccacgacc taatcgtgat
      301 gggctcccac ggccacggcc gcgtcctccg cgccttgctc gggtcggtct ccgcgaaggt
      361 cctcgagaag gcaaccctcc cggtgatgat cctgcgggac gggaagaaca aggaagacat
      421 cgagggttag cggcgcccct cgaaacgctc gcgtcatccg ggctcctgcg acacccacgc
      481 tccggtccag cggggttgcc gtggcagaag ctcattcacc cagatcgagg tgagcaggct
      541 cggtccgcgg aagctgacta gcttcgggac cctgagcagc ggaatcgttg gagcggttgg
      601 ggacccctct cgcagaggct caaggaacca cgttggcgcc tccgctgctc gtttcagctg
      661 cgcgacggtc tctgagagag gggtccccaa ccgctcaagc caacccctct ctcagtctcc
      721 cgatctattc gccttccccg cccagcctgt acgccacgat ctggggtttc tgtccttcgg
      781 ccacggccaa tcctgccgcg gcgatcaggg tgtcgccgga gatggagacg ccgctgttgg
      841 tgcccgcggg cagctgaccg ctccacactt cgccgccggt tttggcgtcg aaggcgtgga
      901 cgatgccttc gaagctggtc gcgaagacga ggtcgttggt cgcggtcgtg gagccgtagg
      961 cggcaccttc gaattcggcg ttccacagtt ctttgccggt ggcgaggtcg agggcgacca
     1021 cttcaccgcc cgggccttcc ggttcggcgc cggcgatcgt ggtgcctttg gagacggtca
     1081 ccggcgcgtt gacgaccggc acgaagacgg ccgtctggtt ggtcgaaatc ggcgcgatca
     1141 cgccacccag atagcccggg tatacgagcc catctttcag cttcgaatat tcgccgcgca
     1201 tcgccagcag gccgtcgtcg tcgcggccgt tgtgtttgcc gaccggggtc gaccagaccg
     1261 gcttgccggt cttcgcgtcg agcgcggtga cgaagcccga cttacccgag ccgattgcca
     1321 gttccttgcc cccggcgctg accaggacgg gcgggttctg gaagtcccag tcgtagaggt
     1381 cgtgcggggt ctgctggtag aaccagtcga gcttgccggt tttcgcgtcg agcttgacca
     1441 ccgagttggt gtagaggttg gggccgggcc ggcttttgcc ccagggatcg gagggcgtgc
     1501 cggggaacgg cgccgggttg ccggtcccga agtacatcga gcctttgccg tcaaaggagg
     1561 gcgggtacca gaggccgccg ccggagttct gtttcttgtc cccccagagg ctcggcggca
     1621 cggtgttgaa gctccatttc tctttgccgc tgttggcgtc gagcgcgtag agcgtgccga
     1681 ccccgccgcc cgggtactgg gaggcctgga tcgccggcac ggtcgagacg tagaccagcc
     1741 cttcgctggt cccgggcgcc atgtcgatcg cttccccgcc cgcgtttttc accagttcca
     1801 ccgaccacag ctctttgccg gtttcctggt cgagcgcgaa ggccccgttg gcggtcgccc
     1861 cgaacacctt gccttcggcc actgcaaccc cgttcgggcc gccggtcggt tcttcgtagc
     1921 gcttggtcca gagcacctcg ccgctctcga ggtcgatcgc ctgcacgttc gactcgaggt
     1981 cctgcgagta gacgacgccg ttggcgatca ccggggtcga ggcgtaggcg ccgaagctgc
     2041 tttccgcggt cagcggcagt ttccaggcca ccgccaggga ggtcgcgttg tcgcgtttga
     2101 tcgtgctgtc ggcgctgcgg gtgttggcgt tgtcgatgcc gggatagccg gtgccgtcga
     2161 gcgtggcgcc gccgtcggag ccgctgtcgt cagaacctcc cccacacccg gcaaccaccg
     2221 ccagggcgac cgcaaatagc gccaaaaggc tgaagacacg gaacctcctg cttgcaagca
     2281 actgcgacat cacaactcct cccctagtcg atcaggactg atttgcgctg ctgtggaccg
     2341 gagcccgaag ggctctgccc gagccccggc agcgccggct gtaagacacg gttggcgagc
     2401 agcccgaggc tgcccaagtt ggcgaacccc ggcccctggc tgaggccgcc gaggcccggg
     2461 atgtgcaggc ggggctccat cccgaccagc tccagggcgc ggccgatcgg gacttcggtc
     2521 tgcggcgggc ggtcccagag cgggcctgcg aggcgctcga cctcggcccg caggccctcg
     2581 tcgaagagac cgcggatctg cgccagcagg tcgaacccgg tgcagttgat cacgtagtcg
     2641 tagggctcgg cgacgacccc gtcggcgccc tcgtattcga gcgccagctt gtcgccctcg
     2701 gcgccgacgt ggacggcgcg gccggtcagg aacgagcact ggcggtcgcc ggcgatcgag
     2761 gccagcaccc ccatgtcgaa gacgccgcgg tcgcagtgct tgacgaagtc ccgccgggtc
     2821 tgcaggtcga gccgctccca gccgacgtcg tcggggtcgg cgaagacgcg gttctcgagg
     2881 aagctctcgc cgcggctcag cggcagggtc ggcgtgtaga cggtgatccg cacccgcggg
     2941 cggaaggagc gcaggaagac gagggcgctg agcgcgctct cccctccccc gacgatcgcg
     3001 atctcgtgat cggcgtcgtc cgggatgcgg gcgaactcgt cgcggcggct gtcgcagtgg
     3061 aagacgcggt cggcgaccgc ggggtcgtgg gcgaactcgc ggtggatgcc gggaccggtg
     3121 aggacaaggg cacgaccgcg gtggcgaacc gggtcggtgg gctcaccgcg ctcggagacc
     3181 tcgacctccc aggcgtcgcc ctcgggggtc agctcgacgc tgctgacgcg gccgtggaac
     3241 ttggcgatcc cctcggtcgc gcgctccagc acccagctca ggtaggcgcc gtagtcgcgg
     3301 tggcggatcg gcggcgattc gcggttgacc caggcggcgt agatgccctg ttcgaccgcg
     3361 tagcgctgcc aggtgaaggg caggagaccg gcgtcgatcg cgtccccggt ctcgccgaac
     3421 tggcggtagg agcggtaggg aaagccgacg tccttgatcg gcgggatcgc cagcggctcc
     3481 tcgcccgagg tcatcccgtt gcgcccgagc caggaggccg ccggctcggt tttctcgacg
     3541 atcgtcacgc tcagcgaccc gagcccgagc cggttgatca cgtggacctt ggccgagatc
     3601 gccgccgcct tggcgccggc gccgacgatc agcaggtcgg actcggtgat cttctgctcg
     3661 gcgctcatcg cacgcaccta ggcggccgcg agggtcgagt tggcgggctg tccggcctga
     3721 cgcggttcgg cgatcacgcg ggcgctgcga tcggggctga acgagatcgc gcttttgcgc
     3781 atcccctccg ggctgtcgtc ggcgggccgc agctcgacct cgccgagcac cgtgcgcacc
     3841 acccgcttca tctccaattc cgcgtagctg gcggcgaggc agcggcgcac gccgccgccg
     3901 aacgggatcc acgagtaggt cggcgccggg ccgtcgagga agcgctcggg caggaactgg
     3961 tcggggtccg ggtagacctt cgggtcgttg tggatcagcg gtacgcaggg ggcgacgac
//
LOCUS       DASRIG010000467         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3321463, whole genome shotgun sequence.
ACCESSION   DASRIG010000467 DASRIG010000000
VERSION     DASRIG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3321463"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFB52_12590"
     CDS             complement(<1..606)
                     /locus_tag="VFB52_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZO07217.1"
                     /translation="MTTTNDETGEEQGRRTRDGQHVTRVHDELRNRILRGEMQPGTSM
                     SQVELAELLDAGRTPLREALRLLQSEGLIENEPNRNVRVAEFTLDDVEDLYLMRISLE
                     CSAALVTVPELAPSDIAELGGLITQMEYFETEREHVGWETPHRAFHLKLVSGAGPQTR
                     RTIEQLWDHAERYRRFYELADPPPWLLRVAEHRGLLDAAKAG"
     gene            717..1547
                     /locus_tag="VFB52_12595"
     CDS             717..1547
                     /locus_tag="VFB52_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZO07218.1"
                     /translation="MAGPEANSSASAQRVRFAGRWFDVYDLSQTLSNETAAIEPNPHS
                     IEYTTHAQSAAAERFGIPAAAWPGEEAWALETVTLTTHSGTHIDAPFHYASTGPGGKP
                     ARTIDELPLQWVMSDAFVLRMIDVEVEVGITERDVREELERIGYAPKENDIALVRTDV
                     SRRFGERGYEALHPGLRREATAYLVEHGVRLIGIDAWGVDRPFGIMAAELHEGDAGQL
                     WESHKYGAEREYSQIEKLCNLAALPQPHGFQVLAMPVAIAGGSAGWARVVALVETKES
                     "
     gene            1547..2479
                     /locus_tag="VFB52_12600"
     CDS             1547..2479
                     /locus_tag="VFB52_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO07219.1"
                     /translation="MIWATYRSPADAGERAGLLLDGKLRGLPAEQQLIDLIGPTGISL
                     EDAAESAVEAPAEVVDPDAVELLAPIPRPPAVRDFMAFENHYVDTRNGLGLKVEPVFY
                     EQPCFYFTNPAAIIGPRTDARVAPGTAQFDFELEIGAIIGRPGSDLSLESAESHVAGY
                     TIFCDWSARDLQATEAPFGTGPAKGKDTASSLGPWLVTPDELESARSERGYDLAMSAF
                     VNDIQYSAGNWSSIYWSMPQLLVFASRGTELRTGDVIATGTVGTGSILELSAVNGHER
                     FPWLVPGDEVRLSVERLGEIRATVVPGPAPLPLS"
     gene            complement(2506..3693)
                     /locus_tag="VFB52_12605"
     CDS             complement(2506..3693)
                     /locus_tag="VFB52_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO07220.1"
                     /translation="MGATSTRPSSEIDLFSDESVLDPYENYRQLRDVAPAVYLTATDA
                     WAISRFDDVREVLRDWERFTSAKGVALNDPMNEANVGGIIASDPPLHTKLRRVLAERL
                     SPKAIDALTDRINQEADELVTAVIAKGSFDGVQDLAGIFPIQVVADLIGLPDEGRDRL
                     LEWANAVFDAFGPDNERSATGLPLVGEMWQYVGEVATRDRLAPGSMGLAVYEAADRGE
                     IEPESCIMLMGGYVAAGMDTTINAIGTGLQLLATHPEQWQALKSDPGLVRSAGNEILR
                     YESPVQVFARTATADQEVSGQLVPEGERVLVLYGSANRDERKYEDPDRFDVTRNPIDH
                     VGLGHGIHRCAGAALARLEIEAILSSLINRVEEIRLEGEPVRQPNNTVRGLRNLPLSV
                     TVS"
     gene            3789..>4004
                     /locus_tag="VFB52_12610"
     CDS             3789..>4004
                     /locus_tag="VFB52_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07221.1"
                     /translation="MQIELPREAETTNASPLWEPTQEEVENAALTRFAEWLARERGVQ
                     TDGYRELWQWSVDEPDAFWGALWDYFEI"
BASE COUNT          617 a         1368 c         1383 g          636 t
ORIGIN      
        1 gccggccttg gcggcgtcga gcaggccgcg gtgctcggcg acgcgcagca gccagggcgg
       61 cgggtcggca agttcgtaga agcgccggta gcgctcggcg tggtcccaga gctgttcgat
      121 cgtgcgccgc gtctgcggcc cggcgccgct gaccagcttc aggtggaagg cccggtgggg
      181 cgtctcccag ccgacgtgtt cgcgctcggt ttcgaagtac tccatctggg tgatcaggcc
      241 acccagttcg gcgatgtcac tgggagccag ctcgggcacc gtgaccaagg cggcgctgca
      301 ctcgagcgag atccgcatca ggtagaggtc ctcgacgtcg tcgagggtga attcggcgac
      361 ccgcacgttg cggttcggct cgttttcgat cagcccctcg ctctgcagca gccgcagcgc
      421 ctcgcgcagc ggggtgcggc cggcgtcgag cagctcggcg agttcgacct gcgacatcga
      481 ggtgcccggc tgcatctcgc cacgcaggat ccggttgcgc agctcgtcgt ggacccgggt
      541 gacgtgctgg ccgtccctgg tccgtcgccc ctgctcttcc ccggtctcgt cgttggtggt
      601 cgtcattgga ttgaagtatg agcgttggtg ccgattgaga aaatgcggtc agtaggataa
      661 gtcaccggct acatcagcgt ttgcatccaa aattgcagtt tgcctataat accggcatgg
      721 ccggccccga ggcgaattcg agcgcgagcg cgcagcgggt ccgcttcgcg ggtcgctggt
      781 tcgacgtcta cgacctcagc cagaccctct ccaacgagac cgcggcgatc gagccgaacc
      841 cgcactcgat cgagtacacg acccacgccc agtcggccgc cgcggagcgc ttcgggatcc
      901 ccgccgccgc ctggcccggc gaagaggcct gggccctgga gacggtcacc ctcaccaccc
      961 actccggcac gcacatagac gcgcccttcc actacgcctc caccgggccc ggggggaagc
     1021 cggcgcgaac gatcgacgag ctgccgctgc agtgggtgat gagcgacgcc ttcgtcttgc
     1081 ggatgatcga cgtcgaggtc gaggtcggaa tcaccgagcg cgacgtgcgc gaggagctcg
     1141 agcggatcgg ctacgcgccg aaggagaacg acatcgccct ggtccgcacc gacgtctcgc
     1201 ggcgcttcgg cgagcgcggc tacgaggcgc tgcaccccgg cctgcgccgc gaggcaaccg
     1261 cctacctggt cgagcacggg gtgcggctga tcgggatcga cgcctggggc gtcgaccggc
     1321 cgttcgggat catggccgcc gagctgcacg agggcgacgc aggacagctg tgggagtccc
     1381 acaagtacgg cgccgagcgg gagtacagcc agatcgagaa gctgtgcaac ctcgcggcgc
     1441 tcccccaacc acacggattc caggtactgg cgatgccggt cgcgatcgcc ggcggcagcg
     1501 ccggctgggc gcgggtcgtc gccctggtcg agacaaagga gagctgatga tctgggccac
     1561 ctaccgttca cctgcggacg ccggcgagcg agcgggcttg ctgctcgacg gaaagctccg
     1621 cgggctgccc gccgagcagc agctgatcga cctgatcggc ccgacgggga tctcgctgga
     1681 ggacgcggcg gagagcgccg tcgaggcgcc ggccgaggtc gtcgaccccg acgcggtcga
     1741 gctgctcgcc ccgatccccc ggccgcccgc ggtccgcgac ttcatggcct tcgagaacca
     1801 ctatgtcgac acccgcaacg gcctcggcct caaggtcgag ccggtcttct acgagcagcc
     1861 ctgcttctac ttcaccaatc cggcggcgat catcgggccg cgaacggacg cgcgggtggc
     1921 gccggggacc gcccagttcg acttcgagct ggagatcggc gcgatcatcg ggcggccggg
     1981 ctccgacctc tcactggaga gcgccgagtc ccacgtcgcc ggctacacga tcttctgcga
     2041 ctggagcgcc cgcgacctgc aggccaccga ggcgccgttt ggaaccggcc ccgccaaggg
     2101 caaggacacc gcatcgagcc tcggcccctg gctcgtcacc ccggacgagc tggagtcggc
     2161 gcgcagcgag cgcggctacg acctcgcgat gagcgccttc gtcaacgaca tccagtacag
     2221 cgccgggaac tggagctcga tctactggtc gatgccgcag ctgctggtct tcgcctcgcg
     2281 ggggaccgag ctgcggaccg gcgacgtgat cgcgaccggg accgtcggca ccggctcgat
     2341 cctcgagctc tcggcggtca acgggcacga gcgcttcccc tggctggtgc cgggagacga
     2401 ggtccggctc agcgtcgagc ggctcggcga gatccgcgcg accgtggtcc ccgggcccgc
     2461 gcccctgccg ctcagctgag cggcaggggc tggcatccgg ttcaactagg aaaccgttac
     2521 cgagagcggc aggttgcgca ggccgcggac ggtgttgttc ggctgccgca ccggctcgcc
     2581 ctcgagccgg atctcctcga cccggttgat cagcgagcta aggatcgcct cgatctccag
     2641 gcgcgcgagg gcggcgccgg cgcagcggtg gatcccgtgg ccgaggccga cgtgatcgat
     2701 cgggttgcgg gtgacgtcga agcgatcggg gtcctcgtac ttgcgctcgt cgcggttcgc
     2761 cgagccgtag aggaccagca cccgctcgcc ctcggggacc agctggccgc tcacctcctg
     2821 gtcggcggtg gcggtgcggg cgaagacctg gacggggctt tcgtagcgga ggatctcgtt
     2881 gccggcgctg cgaaccaggc cggggtcgct cttcagcgcc tgccactgct cggggtgcgt
     2941 ggcgagcagt tgtaggccgg tgccgatcgc gttgatcgtc gtgtccatgc cggcggcaac
     3001 gtagccgccc atcagcatga tgcaggactc cggctcgatc tcgccgcggt cggcggcttc
     3061 gtagacggcg agtcccatgc tgccgggagc gagccggtcg cgggtcgcga cttcgccgac
     3121 gtactgccac atctcgccga ccagcggtag cccggtggcg ctgcgctcgt tgtcggggcc
     3181 aaaggcgtcg aagactgcgt ttgcccactc cagcagccgg tcgcgcccct cgtcgggcag
     3241 gccgatcagg tcggccacca cctggatcgg gaagatcccc gccaggtcct gaacgccgtc
     3301 gaagctgccc ttggcgatca ccgcggtgac cagctcgtcc gcttcctggt tgatccggtc
     3361 ggtgagcgcg tcgatcgcct tcggcgagag ccgctcggcg agcacgcggc gaagcttggt
     3421 gtgcaacggc gggtcggagg cgatgatccc gcccacgttc gcctcgttca tcggatcgtt
     3481 caacgcgacc cctttggccg aggtgaaccg ctcccagtcc ctcagcacct cgcgcacgtc
     3541 gtcgaaccgg gagatcgccc aggcatcggt cgcggtcagg tagacggccg gcgccacgtc
     3601 gcgaagttgc cggtagttct cgtatggatc gaggaccgac tcgtcgctga acaggtcgat
     3661 ctccgacgac ggcctcgtgc tcgttgctcc catctcatcc ctttcgtccc tcgggcagaa
     3721 cccaatcttc cggctagctt accaacttag gatataaagt ccgataataa atccaaactg
     3781 acattgagat gcagatcgaa ttgccaagag aagccgagac gaccaatgcc tcaccgctct
     3841 gggaaccgac ccaggaggaa gtcgagaacg ccgcgctgac gcggttcgct gagtggctgg
     3901 cgcgcgagcg cggggtccag accgacggct accgcgagct ctggcagtgg tcggtcgacg
     3961 agcccgacgc cttctggggc gcgctctggg actacttcga gatc
//
LOCUS       DASRIG010000468         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2544255, whole genome shotgun sequence.
ACCESSION   DASRIG010000468 DASRIG010000000
VERSION     DASRIG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544255"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..503)
                     /locus_tag="VFB52_12615"
     CDS             complement(66..503)
                     /locus_tag="VFB52_12615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZO07222.1"
                     /translation="MADETLQMERTFQAPAQAVFDAWTSEEVMRRWFRGREVFETPFA
                     SVDLRVGGAVRVVMRNPEDGTEYGGGGHYTEVDPPNRLAFTWVWDDEAERETQIEIEF
                     EEADGATTVRFTHSNLRDAEAVRSHEDGWGTCFDLLERNVFAG"
     gene            complement(503..634)
                     /locus_tag="VFB52_12620"
     CDS             complement(503..634)
                     /locus_tag="VFB52_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07223.1"
                     /translation="MIAAHVAGMPVEELLPSLAGAGTGLLFVRAWLALRWRDREDRD"
     gene            complement(631..1302)
                     /locus_tag="VFB52_12625"
     CDS             complement(631..1302)
                     /locus_tag="VFB52_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZO07224.1"
                     /translation="MNTPPVVSPDEWSAARDQLLVKEKELTRARDALAAERRRMPRMA
                     VEKEYRFEGPDGPVGLLDLFEGRRQLVVYRFFFEPGVEGWPERGCSGCSFCADQDAHV
                     AHLNARDTTLVNVSRGDQENIERYRARMGWEVPWYTITDDFDVDFGVGEWHGTNAFYR
                     DDEGQIFRTYMINGRGDEALGNPWSYMDMTALGRQEKWEDSPEGYPQTDPYSWWRLHD
                     EYDAA"
     gene            complement(1299..1610)
                     /locus_tag="VFB52_12630"
     CDS             complement(1299..1610)
                     /locus_tag="VFB52_12630"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO07225.1"
                     /translation="MVNDSFKALSHPIRRGIVERLAIGPATVGEATKGFDVSKPAITK
                     HLRVLEETGVITRVIEGRTHRLSLQPGVLEEAAEWMDRQRAIWGRLFDVVDDYLKEEQ
                     K"
     gene            complement(1668..2417)
                     /locus_tag="VFB52_12635"
     CDS             complement(1668..2417)
                     /locus_tag="VFB52_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07226.1"
                     /translation="MDLGLDGRVALVMGASAGIGRAVAAALVREGARVAIASRTEERI
                     QAAAAEIGATPFVADASDSERLARLPIEVTEAMGGPAEILVLNTGGPPPGGALDHGFE
                     EWQDAYASLVLAPRILTGGVVPAMQAAGWGRIVNVGSTTTIEPNPALSLSNVHRAAAV
                     GFLKTLAGEVAADGITVNTVATGRFRTDRMAELYGSIEAVEEAAAAEVPARRLGTPEE
                     YGDLVAFLCSDRAAYITGTVIPIDGGLLRST"
     gene            2531..>2731
                     /locus_tag="VFB52_12640"
     CDS             2531..>2731
                     /locus_tag="VFB52_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07227.1"
                     /translation="MSRLSRSVSKWLLAAVLALAALTALAAPAMAITGVDLSTYKRVG
                     RFDLPEPTRKTPPAGNLLAQEAS"
BASE COUNT          377 a          953 c          964 g          437 t
ORIGIN      
        1 cgaagacgct gcgcgatgtg atcatctacc ccgccatgcg cgaggctccg ggcgagtcct
       61 gagccctacc cggcgaagac gttccgctcg agcaggtcga agcaggtgcc ccagccgtct
      121 tcgtggctgc ggaccgcctc cgcgtcgcgc aggttcgagt gggtgaagcg cacggtggtg
      181 gcgccgtcgg cctcctcgaa ctcgatctcg atctgggtct cgcgctcggc ctcgtcgtcc
      241 cagacccagg tgaaggcgag ccggttgggc gggtcgacct cggtgtagtg gccgccgccc
      301 ccgtactcgg tgccgtcctc gggattgcgc atgaccacgc gcacggcgcc gccgacgcgc
      361 aggtcgacgg aggcgaacgg ggtctcgaag acttcgcgcc cgcggaacca gcggcgcatc
      421 acctcctcgc tggtccaggc gtcgaagacc gcctgggcgg gggcctggaa ggtgcgttcc
      481 atctgcagcg tctcgtcggc catcagtccc gatcctcgcg gtcgcgccag cgcagcgcaa
      541 gccaggcgcg gacgaagagc aggccggtgc cggccccggc cagcgacggc agcagctcct
      601 cgaccggcat cccggcgacg tgcgcggcga tcatgcggcg tcgtactcgt cgtggagcct
      661 ccaccacgag taggggtcgg tctgcgggta gccctccggc gagtcctccc acttctcctg
      721 gcggccgagc gcggtcatgt ccatgtagga ccaggggttg ccgagcgcct cgtcgccgcg
      781 cccgttgatc atgtaggtgc ggaagatctg accctcgtcg tcgcggtaga aggcgttggt
      841 cccgtgccac tcgccgactc cgaagtccac gtcgaagtcg tcggtgatcg tgtaccaggg
      901 cacttcccag cccatccgcg cgcggtagcg ctcgatgttc tcctggtccc cgcgggagac
      961 gttgacgagc gtggtgtcgc gcgcgttcag gtgggcgacg tgggcgtcct ggtcggcgca
     1021 gaacgagcag ccgctgcagc cgcgctcggg ccagccttcc acgccgggct cgaagaagaa
     1081 gcggtagacg acgagctggc ggcgcccttc gaagaggtcg agcaggccga ccggcccgtc
     1141 cggtccctcg aagcggtact ccttctcgac cgccatccgc ggcatgcggc ggcgttcggc
     1201 ggcgagcgcg tcgcgggccc gggtcagctc cttctccttg accagcagct gatcgcgagc
     1261 ggcgctccac tcatcgggtg agacgacggg tggcgtattc atttctgttc ctccttcagg
     1321 tagtcgtcga cgacgtcgaa caggcgcccc cagatcgcgc gctggcgatc catccactcg
     1381 gccgcctcct cgagcacgcc gggctgcagc gagagccggt gcgttcggcc ctcgattacg
     1441 cgggtgatca cgccggtctc ttccagcacc cgcaggtgct tggtgatcgc cggcttggag
     1501 acgtcgaacc ccttggtcgc ttcgccgacc gtggcgggac cgatggcgag ccgttcgacg
     1561 atcccgcgcc ggatcggatg ggagagggcc ttgaacgaat cattaaccat aaggttaact
     1621 atacagacag cgaaaacggg gtcgtggggg gagggcgccc tacgacctca ggttgagcgg
     1681 agaaggccgc cgtcgatggg gatcaccgtt ccggtgatgt aggcggcgcg gtcggagcag
     1741 aggaaggcga cgaggtcgcc gtactcctcg ggggtgccga ggcgacgggc ggggacttcg
     1801 gcggccgccg cctcctcgac cgcctcgatt gagccgtaga gctcggccat ccggtcggtg
     1861 cggaagcgac cggtggcgac ggtgttcacg gtgatgccgt cggcggcgac ctctccggcg
     1921 agcgtcttca ggaagccgac cgcggccgcc cggtggacgt tggagaggct gagggccggg
     1981 ttcggctcga tcgtcgtggt cgagccgacg ttgacgatcc ggccccagcc ggccgcctgc
     2041 atcgccggga caacgccgcc ggtgaggatc cgcggggcga gcaccaggga ggcgtaggcg
     2101 tcctgccatt cctcgaagcc gtggtcgaga gcaccgccgg gcggcgggcc gccggtgttg
     2161 aggacgagga tctcggccgg gccgcccatc gcctcggtta cctcgatcgg cagccgcgcg
     2221 agccgctcgc tgtcggatgc gtcggcgacg aacggcgtcg ccccgatctc cgcggcggct
     2281 gcctggatcc gctcctcggt tcggctggcg atcgcgaccc gggcgccttc gcggacgagc
     2341 gccgcggcga cggcccggcc gatcccggcg ctcgcgccca tcaccagcgc cacgcgtccg
     2401 tcgagcccga ggtccattcc gcacaatttt gtcaccgttt cttcgcaggt cggtaacgaa
     2461 tctggatcgg gtcggcagcg agtgttccgg ccacccgctc gtacgttcgt ctacccctcc
     2521 ggaggctccc ttgtcccgtc tgtcccgcag cgtctcgaag tggctgctcg ccgccgtcct
     2581 cgcgctggcc gccctgacgg cgctcgccgc gccggccatg gcgattaccg gcgtcgacct
     2641 ctcgacctac aaacgagtcg gccgcttcga cctcccggag ccgacgcgca agactccgcc
     2701 ggcgggcaac ctgctcgcgc aggaggcctc g
//
LOCUS       DASRIG010000469         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1527429, whole genome shotgun sequence.
ACCESSION   DASRIG010000469 DASRIG010000000
VERSION     DASRIG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527429"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFB52_12645"
     CDS             complement(<1..605)
                     /locus_tag="VFB52_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZO07228.1"
                     /translation="MSNRTYVIGVGMTKFEKPGTKEGDYPDWAKEAGEKALADAGIPY
                     EDVEQAYAGYCYGDSTYGQRALYQLGLTGIPVVNVNNNCSTGSSALFLARQAVKGGLV
                     DVALALGFEKMEPGSLGVKYTDRTNALDKHAMRMFEIRDPEQSPPAPQMFGNAGRDHM
                     AKYGSNPDHYAWIGWKNHKHSVNNPYAQFQDEYSLEDIKAA"
     gene            complement(665..1813)
                     /locus_tag="VFB52_12650"
     CDS             complement(665..1813)
                     /locus_tag="VFB52_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZO07229.1"
                     /translation="MSTRSPSTPSPGLFNAAGTARYLRASPLFAIGPAEPGPAIRRGF
                     LIAVPIGVCLGIEFGFDAPTHGAVGTGALFAGFVGMDAGALPRAAWQAAVTPAVAIAA
                     ALGILSSQSAPLAVLAMGLVGGLAGYFFAYSLRLAIYGLSVALALMIAQGFFLPVDET
                     LPAFAWVAIGGLSQVAWSLAVWVFWEHRARGEPSGWDWREVRKTLRDNLTLESDALRH
                     GIRFGGALATGVAVYRIFDMDDHGFWIPLTILFVMRPDRDETYLRLALRAIGTVLGLV
                     LATVAAETIDSDAVTVLILFLAAGFCFGLLTVQYALFTTAVTVYVVLLTDTLGENAWE
                     AADLRAIGTAVGILIAFLAWVLWPNKGGRGLEFEALRPPPPAPEAESR"
     gene            1839..>2180
                     /locus_tag="VFB52_12655"
     CDS             1839..>2180
                     /locus_tag="VFB52_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZO07230.1"
                     /translation="MVFAEISSDPAAGVGFFAALAAGIVSFLSPCVLPLVPGYLSAVT
                     GVSATELENANWRRVLGPSLLFVASFSAIFVLLGLTATGIGSFLDDNIDLLETISGVL
                     IILMGVLFVASL"
BASE COUNT          335 a          744 c          754 g          347 t
ORIGIN      
        1 tttgccgcct tgatgtcctc gagcgagtac tcgtcctgga actgggcgta agggttgttg
       61 accgagtgct tgtggttctt ccacccgatc caggcgtagt ggtcggggtt ggagccgtac
      121 ttggccatgt ggtcgcggcc cgcgttgcca aacatctgcg gcgccggcgg cgattgctcg
      181 gggtcgcgga tctcgaacat ccgcatcgcg tgcttgtcga gcgcgttggt gcggtcggtg
      241 tacttgaccc cgaggctgcc tggctccatc ttctcgaagc cgagcgccag agcgacgtcg
      301 acgagaccgc ctttgaccgc ttggcgagcg aggaacagcg ccgaggagcc ggtcgagcag
      361 ttgttgttga cgttgacgac cgggatcccg gtgaggccga gctggtagag cgcccgctgg
      421 ccgtaggtcg agtcgccgta gcagtagccg gcgtaggcct gctcgacgtc ctcgtacggg
      481 atgccggcgt cggcgagcgc cttctccccc gcctctttgg cccagtcggg gtagtcgccc
      541 tccttggtgc ccggcttctc gaacttggtc atgccgacac cgatcacgta agtcctgttg
      601 ctcaacgtct gctccctaag tacgccgcgt cccgcgcggg ctgtttggct gactagccag
      661 gaatttagcg cgactcggct tccggcgcgg gtggaggcgg cctcagcgcc tcgaattcga
      721 gccccctccc ccccttgttc ggccacagaa cccaggcgag aaaggcgatc aggatgccga
      781 ccgcggtccc gatcgcgcgc aggtcggcgg cctcccaggc gttctcaccg agcgtgtcgg
      841 tgagcagcac cacgtagacg gtgaccgcag tcgtgaacag cgcgtactgg accgtgagca
      901 ggccaaaaca gaagccggcg gcgaggaaga ggatcaggac cgtgacggcg tcgctatcga
      961 tcgtttcggc ggcgacggtg gccaggacca ggccgagcac ggtgccgatc gcccgcaggg
     1021 ccaggcgcaa atacgtttcg tcgcggtcgg gccgcatcac gaagaggatc gtcagcggga
     1081 tccagaagcc gtggtcgtcc atgtcgaaga tccggtagac ggcgaccccg gtggcgagcg
     1141 cgccgccgaa gcggatcccg tggcgaagcg cgtcggattc gagcgtgagg ttgtcgcgca
     1201 gcgttttgcg gacctccctc cagtcccagc cgctcggctc gccccgcgcc cggtgctccc
     1261 agaagaccca gacggccagc gaccaggcga cctgactgag cccgccgatg gcgacccagg
     1321 cgaaggccgg caaggtctcg tcgaccggca ggaagaaccc ctgggcgatc atcagcgcca
     1381 gcgcgaccga gaggccgtag atcgcgaggc gcagcgagta ggcgaagaaa tagccggcga
     1441 gcccgccgac caaccccatc gccagcaccg ccagcggcgc cgactggctg ctgaggatgc
     1501 cgagtgcggc ggcgatcgcg accgccgggg tgaccgcggc ctgccaggcc gcgcgcggca
     1561 gcgccccggc gtccatcccg acgaagccgg cgaacagggc gccggtcccg accgcgccat
     1621 gggtcggcgc gtcgaaaccg aactcgatcc cgaggcagac gccgatcggg accgcgatca
     1681 ggaagccgcg gcggatcgcc gggccgggct cggcggggcc gatcgcgaac agcggcgacg
     1741 cgcgcaggta gcgcgcggtc ccggcggcgt tgaagaggcc cggggacgga gtggagggcg
     1801 agcgggtcga caaggccctg agggtaacct cagcacccgt ggtattcgcc gagatcagct
     1861 ccgatcccgc tgcaggcgtc ggttttttcg ccgccctcgc cgccgggatc gtctccttcc
     1921 tctccccctg cgtgctgccg ctcgtgcccg gctacctctc ggcggtgacc ggcgtctccg
     1981 ccaccgagct cgaaaacgcc aactggcggc gcgtgctcgg gcccagcctg ctcttcgtcg
     2041 ccagcttctc ggcgatcttc gtcctgctcg ggctgaccgc gaccgggatc ggctccttcc
     2101 tcgacgacaa catcgacctg ctggaaacga tctccggggt cctgatcatc ctcatgggcg
     2161 tcctcttcgt cgcctcgctg
//
LOCUS       DASRIG010000470         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1527514, whole genome shotgun sequence.
ACCESSION   DASRIG010000470 DASRIG010000000
VERSION     DASRIG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527514"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1417
                     /gene="lpdA"
                     /locus_tag="VFB52_12660"
     CDS             50..1417
                     /gene="lpdA"
                     /locus_tag="VFB52_12660"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZO07231.1"
                     /translation="MPENYETIVIGGGPGGYVAAIRSAQLGRKTAVVERDKAGGRCLN
                     YACIPAKTILRSAEIYHEAKNSENLGVVAKDVEIDWPALQQRRADVSESLSKGVSGLW
                     KKNKIDVIEGDGKLDKDGNVVVGDTTYEAGAVILATGSVALPIPGVEFGERVVDTWGA
                     WSLPGLPKKIAVVGAGASGSEIASAYARMGTEVLLIEMLPQILPAEDADIAKIVERSF
                     KKDGIEISTGAPVENVEVSGDSVKFTYGDNSAEVDYLCIAGGRRPDVEGLGLEDAGVQ
                     LEENGKIKVDGYGQTTNAKVYAIGDLVNKKALAHKASEEGVVAVEKAAGVETHPVDQE
                     LMVGATFTHPQVASVGLTEAQAKEAGHEVKVGKQKIAGEGAGTVYDDKVGLVKLVVDA
                     KYGEVLGAHIVGNSACDMIAELVATMALEGGYQELSRIVHPHPTVSEAILDAARAVDG
                     WAIHA"
     gene            1425..2012
                     /locus_tag="VFB52_12665"
     CDS             1425..2012
                     /locus_tag="VFB52_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZO07232.1"
                     /translation="MRATFYYDLGSPYAYLSAERISGLYTEAGLEQPEWVPVLLGGLF
                     AQFGRSSWGQGPDREEGMAECERRASAYGLPPIVWPDPWPGNMLTAMRVATFAKQTGR
                     AVSFSLAAFRQQFAAGRDLSEPDNVMIAGAACELHPRALLKAVETGGVKAALRAATEG
                     AATQGVEGVPAVVVGEQVFWGDDRLEEAVEASASG"
     gene            2025..3257
                     /gene="pdhA"
                     /locus_tag="VFB52_12670"
     CDS             2025..3257
                     /gene="pdhA"
                     /locus_tag="VFB52_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZO07233.1"
                     /translation="MPGSLPDASTCYELLARMALIRRFEEEAGRQYQRAKAGGFLHLA
                     IGEEATIVGTASVMRPEDFLIGTYRTHGHAIARGTDPKRVMAELFGRVDGTSGGRGGS
                     MHIFDAEKRFMGGYGIVGGNLPLAAGLALASQYKSEDAVTVCMFGDGASNTGNFGETM
                     NLAALWTLPVLFLVENNLYGMGTAVERHSAQTDLSKKAEGYGISGTRVDGMDVIAMRE
                     AVEAGLRAAREDERPTLLEAFTYRYRGHSAADPEVYREREEVEEWREKDPIETFAKRC
                     VDEGVLGEREVKQARETADEVVEEAVEFADDSPEPPLDTMYENLYARTEPGGWYSVDE
                     RSPEPHRGEDEAEMPAAARELAEAGAAYADVEDEEGRPNPAIQGDAQDGHASRRGSEP
                     LNADEGDGEPAEAEEDAR"
     gene            3257..>3950
                     /locus_tag="VFB52_12675"
     CDS             3257..>3950
                     /locus_tag="VFB52_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO07234.1"
                     /translation="MAELRMREALRDAMAEEMRRDESVFVMGEDVAVFQGAFKVTEGL
                     LDEFGERRVRDTPISENTIVGAGVGAAMNGLRPIVELMTVNFSLLALDQIVNHAAAIP
                     YMFNGQVKVPLVIRMPGGGGHQLGPTHSHSFEALYLQVPGLLVACPSTPADAKGLLKA
                     AIRDDNPVVFIEHESLYGVKGEVADGDGEPLRFGEAAVRREGADVTLVGILKMAQVAE
                     EAAQVLADEHGVS"
BASE COUNT          671 a         1347 c         1416 g          516 t
ORIGIN      
        1 ctccaaatag acggagaagc cggcgcggtc ccttctatag gctcagccca tgcctgagaa
       61 ctacgagacg atcgtcatcg gcggcggccc tggtggctac gtcgccgcca tccgctccgc
      121 ccagctcggc cgcaagaccg ccgtcgtcga gcgcgacaaa gcgggcggcc gctgcctcaa
      181 ctacgcctgc atccccgcga agaccatcct tcgctccgcc gagatctacc acgaggcgaa
      241 gaacagcgag aacctcgggg ttgtcgccaa ggacgtcgag atcgactggc ccgctctgca
      301 gcagcgccgc gccgacgtct ccgaatcgct ttccaagggc gtctcgggcc tctggaagaa
      361 aaacaagatc gacgtgatcg agggcgacgg caagctcgac aaggacggca acgtcgtcgt
      421 cggcgacacg acctacgagg ccggcgccgt gatcctcgcg accggctcgg tggcgctgcc
      481 gatcccgggc gtcgagttcg gcgagcgcgt cgtcgacacc tggggcgcct ggtcgctgcc
      541 cggcctgccg aagaagatcg ccgtggtcgg ggccggtgcc tcgggctcgg agatcgcctc
      601 cgcctacgcg cgcatgggca ccgaggtgct gctgatcgag atgctgccgc agatcctccc
      661 cgccgaggac gccgacatcg ccaagatcgt cgaacgctcc ttcaagaaag acgggatcga
      721 gatctcgacc ggcgccccgg tcgagaacgt cgaggtctcg ggcgactcgg tcaagttcac
      781 ctacggcgac aactccgccg aggtcgacta cctctgcatc gccggtggcc gccgccccga
      841 cgtcgagggg ctcgggctcg aggacgccgg cgtccagctc gaggagaacg gcaagatcaa
      901 agtcgacggc tacggccaga cgaccaacgc caaggtctac gcgatcggcg acctggtcaa
      961 caaaaaggcg ctcgcccaca aggcctccga ggagggcgtg gtcgcggtcg agaaagcggc
     1021 cggcgtcgag acccaccccg tcgaccagga gctgatggtc ggcgccacct tcacccaccc
     1081 gcaggtcgcc agcgtcgggc tgaccgaggc tcaggccaaa gaggccgggc acgaggtcaa
     1141 ggtcggcaaa cagaaaatcg ccggcgaggg cgcgggaacc gtctacgacg acaaagtcgg
     1201 cttggtcaag ctggtcgtcg acgccaaata cggcgaggtc ctgggcgccc acatcgtcgg
     1261 caacagcgcc tgcgacatga tcgccgagct ggtcgcgacg atggcgctgg agggcggcta
     1321 ccaggagctc tcgcggatcg tgcacccgca cccgacggtc tccgaggcga ttctggatgc
     1381 ggcccgggcc gtagacggct gggcgattca cgcataatcg ccctgtgcgg gcgacgttct
     1441 attacgacct cggctcgccg tacgcgtacc tgagcgccga gcggatcagc gggctctaca
     1501 ccgaggccgg gctcgaacag ccggagtggg tgccggtgct gctcggcggg ctgttcgcgc
     1561 agttcggccg cagctcctgg ggccaggggc ccgaccgcga ggaggggatg gcagagtgcg
     1621 agcggcgcgc ctccgcctat gggctgccgc cgatcgtctg gcccgacccc tggccgggga
     1681 acatgctgac cgcgatgcgg gtcgcgacct tcgccaagca gaccggccgc gccgtctcct
     1741 tcagcctcgc cgccttccgc cagcagttcg ccgcgggccg cgacctcagc gagcccgaca
     1801 acgtgatgat cgccggcgcc gcctgcgagc tgcacccgcg ggcgctgctg aaagcggtcg
     1861 agacgggtgg cgtgaaagcg gcgttacggg cggcgaccga aggggcggcc acgcaggggg
     1921 tcgaaggggt gccggcggtg gtcgtcggcg aacaggtctt ttggggggac gaccggctcg
     1981 aggaagcggt cgaagccagc gcatctgggt agtctgaatt ccagatgccc ggttcgctcc
     2041 ccgacgcctc cacttgttac gagctgctgg cgcggatggc cctgatccgg cgcttcgagg
     2101 aggaggccgg acggcagtac cagcgggcga aagccggcgg cttcctgcac ctggcgatcg
     2161 gcgaggaggc gacgatcgtc ggcaccgcct cggtgatgcg ccccgaagac ttcctgatcg
     2221 ggacctaccg cacccacggc cacgcgatcg cccgcggcac cgacccgaag cgggtgatgg
     2281 cggagctgtt cggccgcgtc gacgggacca gcggcgggcg cggcggctcg atgcacatct
     2341 tcgacgccga gaaacggttc atgggcggct acgggatcgt cggcggcaac ctgccgctgg
     2401 ccgctggtct ggcgctcgcc tcccagtaca agagcgaaga cgcggtcacc gtctgcatgt
     2461 tcggcgacgg cgcctccaac accggcaact tcggcgagac gatgaacctg gcggcgctgt
     2521 ggacgctgcc ggtgctcttc ctggtcgaga acaacctcta cgggatgggg accgcggtcg
     2581 agcgccactc ggcccagacc gacctctcga agaaggccga gggctacggg atctccggca
     2641 cccgggtcga cgggatggac gtgatcgcga tgcgcgaggc ggtcgaggcg gggctgcggg
     2701 cggcgcgcga agacgaacgg ccgacactgc tggaggcgtt cacctaccgc taccgcggcc
     2761 actccgccgc cgaccccgag gtctaccgcg agcgcgagga ggtcgaggag tggcgggaga
     2821 aagacccgat cgagaccttc gccaagcgct gcgtcgacga gggcgtgctc ggcgagcgcg
     2881 aggtcaaaca ggcccgcgaa accgcagacg aagttgtcga ggaggcggtt gagttcgccg
     2941 acgactcgcc cgaaccgccg ctcgacacga tgtacgaaaa cctctacgcg cgcaccgaac
     3001 cggggggctg gtactcggtc gacgagcgca gcccggagcc gcaccgcggc gaggacgagg
     3061 ccgagatgcc ggcggccgcg cgcgagctgg ccgaggccgg cgccgcctac gccgacgtcg
     3121 aggacgagga ggggcggccg aacccggcga tccagggcga cgcccaggac ggccacgcca
     3181 gccgccgcgg ctccgagcca ctcaacgccg acgagggcga cggcgagccc gccgaagccg
     3241 aggaggacgc gcgctgatgg ccgagctgcg gatgcgggag gcgctgcgcg acgcgatggc
     3301 cgaggagatg cgccgcgacg agtcggtctt cgtgatgggc gaggacgtcg ccgtcttcca
     3361 gggcgccttc aaggtgaccg aggggctgct cgatgagttc ggcgagcgcc gcgtgcgcga
     3421 caccccgatc tccgagaaca cgatcgtcgg cgccggggtc ggcgctgcga tgaacgggct
     3481 gcgcccgatc gtcgagctga tgaccgtcaa cttctcgctg ctggcgctcg accagatcgt
     3541 caaccacgcc gccgcgatcc cctacatgtt caacggccag gtgaaggtgc cgctggtgat
     3601 ccggatgccg ggcggcggcg gccaccagct cgggcccacc cactcgcaca gcttcgaggc
     3661 gctctacctc caggtccccg gccttctggt cgcctgcccc tcgacccccg ccgacgccaa
     3721 gggcctcttg aaggctgcga tccgcgacga caacccagtc gtcttcatcg agcacgagag
     3781 cctctacggg gtcaagggcg aggtcgccga tggcgacggc gagccgctgc gcttcggcga
     3841 agcggcggtg cgccgcgagg gcgccgacgt gaccctggtc gggatcctga agatggccca
     3901 ggtggccgag gaggcggcgc aggtgctggc cgacgagcac ggcgtctccg
//
LOCUS       DASRIG010000471         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_227603, whole genome shotgun sequence.
ACCESSION   DASRIG010000471 DASRIG010000000
VERSION     DASRIG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227603"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VFB52_12680"
     CDS             <1..1458
                     /locus_tag="VFB52_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HZO07235.1"
                     /translation="RSAEKFAPDAVFGLSDEQVARFLGVMYACDGHVYCSDRLAQIGY
                     TTISERLARDVQHLLLRLGIVATIRTLKRAVYEGTGKVAREVRITSQESLCRFCELVR
                     VPGKEAKQDEVLERLGSAPRSTNTDTLPSNIWEEILFAKGDRSWADVSEVTGRPRNHN
                     WHVGARAPSRGLVGELTTAVVSPALEELCESDIWWDEVESVEYVGEEETYDLDVPGLR
                     NFVADDIIVHNSAIVANIAENVAVKRQLPVAFFSLEMSEVELAQRFIACRARISGDKL
                     RKGQVSQKDWPKVVRACNELEEAPLWFDDSSDLGILDLRAKARRLHAQEQERGGLGLI
                     ILDYMQLMRSDDHRANRVEQVGQISRGLKILARELEVPVVAISQLSRAPEQRSPPKPQ
                     LSDLRESGQIEQDADVVAFLYRADYYRDPDEEPDGLADLIIAKHRNGPIGTKSLVFLD
                     RFPKFADYSGQDQPIEQPAGEGPPLEDAAAAGPDF"
     gene            1458..2195
                     /locus_tag="VFB52_12685"
     CDS             1458..2195
                     /locus_tag="VFB52_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZO07236.1"
                     /translation="MAIARSEPFRDQVCPLGVCDGSGWILGPEDVARPCECRAERLNR
                     GRNRGISSVIPPRYRGVSFDRPPVSDMARDLQTKAAVAEVRKYIDELDARLAEGRGLW
                     LFGDTGTGKTTLAMLISQAALEAGKTVAIYSLPKLLARIRRTYDAEPGGEGYLSFFER
                     LTSVDLLHIDDLGAEKRSDWVLEQLYALVNERYEAQLPVLITTNLDHQELEEQIGSRT
                     VSRLTQMCDEVDVFGEDRRYGKYNKAA"
     gene            2247..3593
                     /locus_tag="VFB52_12690"
     CDS             2247..3593
                     /locus_tag="VFB52_12690"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZO07237.1"
                     /translation="MPGVVIVGAQWGDEGKGKVVDLLAKDATTIVRFQGGNNAGHTIV
                     RDGEEFKFHLIPSGILHSDKTCVIGNGVVVDPRVLLSEIDGLKRAGIGVGNLRLSANA
                     HLIMPYHVLLDQAGETKLGKNSIGTTRRGIGPAYADKALRLGIRVQDLLDEKILRTKI
                     RAALEPKQQALRELSVQRRKRRKEAGEEKGGEAAALEPDPRLDVHAMVEEHVNYGHRL
                     EPHIADTAKLCWDALDGEQMVIFEGAQAALLDLDHGTYPFVTSSNPIAGAACVGAGVG
                     PADIDEVWGIAKAYATRVGAGPFPTELFDEVGERIVERGHEFGTTTGRRRRAGWMDLI
                     ALRYAVRLNRMDSLAITKLDVLSGIGTLRVAVRYRSKEGAVLDSFPYHQSILHSATPE
                     YEELPGFDAELGECRSAADLPQEARDYLGFISEFVGVPVRLVGVGPDRDQTVWLES"
     gene            complement(3619..4224)
                     /locus_tag="VFB52_12695"
     CDS             complement(3619..4224)
                     /locus_tag="VFB52_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07238.1"
                     /translation="MRGAKGECRRLALVCAVVAALTLGLAACGGDDGDSTGASTETSA
                     AAGAPADRDGSGAGGGQGPSAEFEVPGGDNSVPRFGTEASAAQRRVASAVVAGYLKAI
                     AAKDYVAQCRYLDRVSVEQLEAFAGLRPGKGGCGPILGELRVSASGVQAGEMAAPVAS
                     LRVKGTRGFALYHGKGGVDYVMPLAREGGEWKVTSMFPVEL"
     gene            complement(4282..>4672)
                     /locus_tag="VFB52_12700"
     CDS             complement(4282..>4672)
                     /locus_tag="VFB52_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07239.1"
                     /translation="DVQRFGVEASAAERQEAAEAVGSFIEGSAEKRWQPVCQSLSKAI
                     RGSFEEAAVAQGDDGGCPQVLGQIAARTPTYMPEDNMARAVGSLRVEGNTGYALYRGT
                     DGLDYAIPVDREGGHWKVAAMHSLMVS"
BASE COUNT          763 a         1636 c         1565 g          708 t
ORIGIN      
        1 cgctcggcgg agaagtttgc gccggatgcg gtctttggac tctcggatga gcaggttgct
       61 cgcttcctcg gcgtcatgta cgcctgcgac gggcacgtct attgcagcga ccgcttggcc
      121 cagatcggct atacgacgat cagcgagcga cttgcccgcg atgtccagca tctgttgctg
      181 agacttggca tcgtggcgac gatccggact ttgaaacggg cggtctacga ggggacgggg
      241 aaggtcgctc gggaagttcg catcacctct caggagagtc tgtgccgctt ctgcgagcta
      301 gtccgcgttc ccggcaagga agcgaagcag gacgaggtct tggagcgtct tgggtcggct
      361 ccccgatcga cgaacaccga cacgctcccc tccaacatct gggaagagat cctgtttgcc
      421 aagggcgatc gctcgtgggc cgacgtcagc gaggtcaccg gccggccgcg caaccacaac
      481 tggcacgttg gcgcccgcgc tccctcgcgg gggctggtcg gtgagctgac gacagcggtc
      541 gtctctcccg cccttgagga gctttgcgaa tccgatatct ggtgggacga ggtcgagtcg
      601 gtcgagtacg tcggcgaaga agagacgtat gacctcgatg tgcctggatt gcgtaatttc
      661 gttgccgacg acatcatcgt ccacaacagc gcgatcgtcg ccaacatcgc cgaaaacgtg
      721 gcggtcaagc ggcagctgcc ggtcgccttc ttctcgctgg agatgtcgga ggtcgagctg
      781 gcccagcgct tcatcgcctg ccgggcgcgg atctccggcg acaagctgcg caaggggcag
      841 gtctcacaga aggactggcc caaggtggtg cgtgcctgca acgagctgga ggaggcgccg
      901 ctctggttcg acgactcctc cgacctcggg atcctcgacc tgcgggcaaa ggcccggcgt
      961 ctgcacgccc aggaacagga gcgcggcggc ctcggcctga tcatcctcga ctacatgcag
     1021 ctgatgcgct cggacgacca ccgcgccaac cgggtcgagc aggtgggtca gatcagccgc
     1081 ggactgaaga tcctcgcgcg tgagctcgag gtcccagtag tcgcgatctc ccagctctcg
     1141 cgcgcacccg agcagcgctc gccgccgaaa ccgcagctct ccgacctgcg tgagtcgggg
     1201 cagatcgagc aggacgccga cgtcgtcgcc tttctctatc gggcggacta ctaccgcgac
     1261 cccgacgagg agcccgacgg ccttgccgac ctgatcatcg ccaagcacag gaacggcccg
     1321 atcgggacca aatcgctggt cttcctcgac cgcttcccca aatttgccga ctactccggc
     1381 caggaccagc cgatcgaaca gccggccggc gaagggccgc cgctcgagga cgccgccgca
     1441 gcggggcctg acttctgatg gcgatcgccc gctcggaacc cttccgcgac caggtctgcc
     1501 cgctcggcgt ctgcgacggc tcgggctgga tcctcggtcc cgaggacgtc gcccggccct
     1561 gcgagtgccg ggccgaacgg ctcaaccgcg gccgcaatcg cggcatctcc tcggtgatcc
     1621 cgccgcgcta ccgcggcgtc tccttcgacc ggccgccggt ctccgacatg gcgcgggacc
     1681 tgcagacgaa agcggcggtc gccgaggtgc gcaagtacat cgacgagctc gacgcgcggc
     1741 tcgcggaggg gcgcgggctc tggctcttcg gcgacaccgg gaccggcaag acgacgctgg
     1801 cgatgctgat ctcgcaggct gcgctggaag cgggcaagac ggtggcgatc tactcgctgc
     1861 cgaagctgct ggcaaggatc cggcgtacct acgacgccga gcccggcggc gagggctacc
     1921 tctccttctt cgagcggctg acctcggtcg acctcctcca catcgacgac ctcggcgcgg
     1981 agaaacgctc ggactgggtt ctggagcagc tctacgcgct ggtcaacgag cgctacgagg
     2041 cgcagctccc ggtcctgatc acgaccaacc tcgaccacca ggagctcgag gagcagatcg
     2101 gttcgcggac ggtctcgagg ctgactcaga tgtgcgacga ggtcgacgtt ttcggcgagg
     2161 atcgccgcta cggcaagtac aacaaggccg cctgagaggc gaccgccgta cgcccagcgg
     2221 ccgcgaccgt cagaatccca gccgcgatgc caggagtagt catagtcgga gcccagtggg
     2281 gcgatgaggg caagggcaag gtcgtcgacc tgctcgccaa agacgcgacc acgatcgtgc
     2341 gcttccaggg cggcaacaac gccggccaca cgatcgtccg cgacggcgag gagttcaaat
     2401 tccacctgat cccctcgggg atcctgcact cggacaagac ctgcgtgatc ggcaacggcg
     2461 tggtcgtcga cccgcgggtg ctgctgagtg agatcgacgg gctcaaacgg gccgggatcg
     2521 gcgtcggcaa cctgcggctc tcggccaacg cgcacctgat catgccctac cacgtgctgc
     2581 tcgaccaggc gggcgagacg aaactgggga agaactcgat cggcacgacc cggcgcggga
     2641 tcgggcccgc ctacgccgac aaggcgctgc gcctcggcat ccgcgtccag gatctgctcg
     2701 acgagaagat cctgcggacg aagatccgcg ccgcgctcga gcccaagcaa caggctctgc
     2761 gcgagctctc ggtccagagg cgcaaacggc gcaaggaagc cggtgaggaa aaaggcggcg
     2821 aggcggcggc gctggagccg gacccgcgcc tggacgtgca cgcgatggtc gaggagcacg
     2881 tcaactacgg acaccggctg gagccccaca tcgccgacac cgcaaagctc tgctgggacg
     2941 cgctcgacgg cgagcagatg gtgatcttcg agggtgcgca ggcggcgctg ctcgacctcg
     3001 accacggcac ctatcccttc gtcacctcct ccaacccgat cgccggtgcg gcctgcgtcg
     3061 gcgccggtgt cgggcccgcc gacatcgacg aggtctgggg gatcgccaag gcctacgcga
     3121 cccgggtcgg cgccggtccc ttcccgaccg agctgttcga cgaggtcggc gagcggatcg
     3181 tcgagcgcgg ccacgagttc ggcaccacca ccggccgccg ccggcgcgcc ggctggatgg
     3241 acctgatcgc cctgcgctac gcggtgcggc tgaaccggat ggactcgctg gcgatcacca
     3301 agctcgacgt gctcagcggg atcgggacgc tgcgggtggc ggtccgctac cgctccaagg
     3361 aaggcgccgt gctcgacagc ttcccgtacc accagtcgat cctgcactcg gcgacgcccg
     3421 agtacgagga gctgcccggc ttcgacgccg agctcggcga gtgccgcagc gccgccgacc
     3481 tgccccagga ggcccgcgac tacctcggct tcatcagcga gttcgtcggc gtcccggtcc
     3541 gtctggtcgg cgtcggcccc gaccgcgacc agacggtctg gctcgagtcc tgagcgaccg
     3601 ccctgagcgg ggggcgcctc agagttcgac cgggaacatc gaagtgacct tccactcgcc
     3661 accctcgcgg gcaagcggca tcacgtagtc gaccccgccc ttgccgtggt agagggcgaa
     3721 gccgcgcgtg ccttttaccc gcaggctggc aaccggggcc gccatctccc cggcctggac
     3781 ccccgaggcg ctgacgcgca gttcgccgag aatcggcccg cagccgcctt tcccgggccg
     3841 cagaccggcg aaggcctcga gctgttcgac cgaaacgcgg tcgaggtagc ggcactgggc
     3901 gacgtagtcc ttggccgcga tcgccttcag gtagcccgcg accacggctg aggcgacccg
     3961 ccgctgggcg gcgctggcct cggtgccgaa gcgcgggacg ctgttgtccc cgcccggcac
     4021 ttcgaactcg gcgctgggcc cctgcccgcc ccctgcgccc gagccgtcgc gatctgcggg
     4081 cgcgcccgcg gcggcgctgg tctcggtgga ggcaccggtg gaatctccgt catctccgcc
     4141 gcaggcagcc aggccgagcg tcagcgcggc gacgaccgcg cagacgagcg cgagcctcct
     4201 gcactccccc ttcgcccccc tcatggaaaa gacagtttac gcatttggga acgcagcgat
     4261 actcccacct acgccggatc gctagctgac catcagcgag tgcatcgccg cgaccttcca
     4321 gtgaccgccc tcgcggtcga cggggatcgc gtagtcgagg ccatcggtcc cccggtagag
     4381 cgcgtagccg gtgttgcctt ccacgcgcag gctgcctacc gcgcgcgcca tgttgtcttc
     4441 gggcatgtag gtcggggtcc gcgcggcgat ctgaccgagg acttgggggc aaccgccgtc
     4501 gtccccctgg gccaccgccg cttcttcgaa cgaaccgcgg atcgccttgg aaaggctctg
     4561 gcacaccggc tgccagcgtt tctcagcgct tccctcgatg aaggatccga cggcttccgc
     4621 ggcttcctgc cgttcggcgg cgctggcctc gaccccgaac ctctgcacgt cg
//
LOCUS       DASRIG010000472         4344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2834212, whole genome shotgun sequence.
ACCESSION   DASRIG010000472 DASRIG010000000
VERSION     DASRIG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834212"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..909)
                     /locus_tag="VFB52_12705"
     CDS             complement(142..909)
                     /locus_tag="VFB52_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07240.1"
                     /translation="MSADPTMGELFAVLLARDFREEDRSLQVGANLPMARAAAVMASV
                     TSRPDLRVILGMGVNNLSGGRPAPPVYPFLFDPRSMVGEAVMVQERVFDDVARPDVFF
                     VGGLQLDRRGNLNLFGIPDGEGGWKLRGPGGVALATMSTNCAGYYIIMPRHDARVFVE
                     KVALITALGDRTERERLGLPGGGPRFVLSPLGVFDFDESGDMQVRSLHAGVSADQVRE
                     ATGFELGNLDDPPTTEAPSDEELTLLRERVDVERTLA"
     gene            complement(909..1793)
                     /locus_tag="VFB52_12710"
     CDS             complement(909..1793)
                     /locus_tag="VFB52_12710"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZO07241.1"
                     /translation="MSLAAGERTSALATVEEALAGIGDGATVGIGGAVTAAHPMALVR
                     ALAASGVTGLTVVAPTGGLDVELLIAAGCVDTLIAAYVGMEGAAGVAPVYRRAVEDGS
                     VTFKDYDEAHCIAGLRAAAQKLPFMPCRAGVGTSYTEITPDLVGFADPVAGQKLIAVP
                     ALELDVALIFAETADEYGNAQTTSAGHMDSLLGAAAKRVVVQVDRVVSNDEIRKDPLR
                     TWYWKQTQVVRAPFGTHPYSSATMVADVDHLKEAIGAGREGGEALDAYMKRFVHDAPD
                     HDSYLEEIGIRRIASLQI"
     gene            complement(1790..3133)
                     /locus_tag="VFB52_12715"
     CDS             complement(1790..3133)
                     /locus_tag="VFB52_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZO07242.1"
                     /translation="MNLTKLAVGERLGRFVVEVRDQEPPAEVVEKLRCNLLHDFGVAM
                     AAHTEGPAIWPPVEGLGPAEATLLCSGAKVDAEHAAFANAALMHARAQDDTHYAAKTH
                     VGSAVIPAALATAQRTGADGEAFVRAVIAGYEVAASVGERLAAASTARGFRASMLYGT
                     LGAAAASASLLGLDEEGTESAIGIAASFSGGLNQTWIEGTSEWRWELGMAARNGLWAA
                     TLAATGAAGARHWYEGPAGFARAFAGETEDDGEWELGERWRILDVIYKPYPVCNITQS
                     PVEAAISLVAEVDVDPDEVESIRCMLNPEDRSYPGTLNWGPFRDVGASLMSAPFCVAM
                     AVKLRSATLEGLHETEDPVIRELVARTEVLPDERLPTLAARIELTTTTGERHVRELIP
                     DGATYGWEWDGVRENSRRLLAEMAVDEAGLEKLEAAIRGIAELDSVTPLVEATIA"
     gene            complement(3130..3825)
                     /locus_tag="VFB52_12720"
     CDS             complement(3130..3825)
                     /locus_tag="VFB52_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZO07243.1"
                     /translation="MSETAPAAAERPGQGEPEVVKLPMIRRAMARRLAESTTVPVFYL
                     RNSVDVAGVFAERTRLKAEGTGRVPTLNDYMLAAVGKALRENPVVNASWADGEVHRHP
                     RVNVGVAIAVRDGLVVPAIYDADRLGANEIAATTRALVEASEARRLSRDVLKDATFTV
                     SNLGMYGIEDFDPVINPPQAAILGVGTAAPADADGRRLIRLSLGCDHRVLTGAEGANF
                     LASIKSGIEEVGS"
     gene            complement(3834..4073)
                     /locus_tag="VFB52_12725"
     CDS             complement(3834..4073)
                     /locus_tag="VFB52_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HZO07244.1"
                     /translation="MARVDVRIEDPDPEEEVEVVQLNVEVGAKVASGEALMEIASDKA
                     NMDLTSPIDGTVVELHVAEFDIVKADHVFAVVSDE"
     gene            complement(4075..>4344)
                     /locus_tag="VFB52_12730"
     CDS             complement(4075..>4344)
                     /locus_tag="VFB52_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO07245.1"
                     /translation="SPLDRPAIVESVRKTGRVVVAQDATAPCSIASEVCAIAAESCLA
                     ELKTEPVRVTSPFINVPTPVPLAEARAPGVDDIAAALRECAGKGS"
BASE COUNT          569 a         1517 c         1620 g          638 t
ORIGIN      
        1 ggagctgatc gccttctgcg ccgagcgcct cgccgactac aaggtcccga cccagatcga
       61 cttcatcgac gccctccccc gcaacggcat gaaccgcgtg atcaagggcg tcctgaccgg
      121 cgacaacgac tcgctgtcgg cttaggccag agtccgctcg acgtcgacgc gctcgcggag
      181 cagggtcagc tcctcgtcgc tgggggcctc ggtggtcggc gggtcgtcga ggttgccgag
      241 ctcgaagccg gttgcctcgc ggacctggtc ggcgctgacg ccggcgtgca gggagcgcac
      301 ctgcatgtcg ccggactcat caaagtcgaa gacgccgagt ggggagagga cgaagcgggg
      361 gccgccgccg gggaggccga ggcgctcgcg ctcggtgcgg tcgccgagcg cggtgatcag
      421 ggccaccttc tcgacgaaga cgcgggcgtc gtggcgcggc atgatgatgt agtagccggc
      481 gcagttggtg ctcatcgtcg ccagggcgac gccgccgggg ccgcgcagct tccagccgcc
      541 ctcgccgtcc gggatcccga acaggttgag gttgccgcgg cggtcgagct gcaggccgcc
      601 gacgaagaag acgtccgggc gggcgacgtc gtcgaagacg cgctcctgga ccatcaccgc
      661 ctcgccgacc atcgagcgtg ggtcgaagag aaacgggtag acgggcgggg cggggcgtcc
      721 gcccgagagg ttgttgacgc ccatcccgag gatcacccgc aggtccggcc ggctggtgac
      781 gctcgccatg accgccgccg cgcgcgccat cggcaggttg gcgccaacct ggaggctgcg
      841 gtcctcctcg cggaagtcgc gggcgagcag gacggcgaac agctcgccca tcgtcggatc
      901 ggcgctcatc agatctgcag cgaggcgatc cggcggatgc cgatctcctc gaggtaggag
      961 tcgtggtcgg gtgcgtcgtg gacgaagcgc ttcatgtagg cgtcgagcgc ctcgccgccc
     1021 tcgcggccgg ccccgatcgc ctccttcagg tggtcgacgt cggcgaccat cgtcgcgctc
     1081 gaatatgggt gcgtgccaaa gggtgcgcgc acgacctggg tctgtttcca gtaccaggtg
     1141 cgcagcgggt ccttgcggat ctcgtcgttg gagacgaccc ggtcgacctg gacgacgacc
     1201 cgcttcgcgg cggcgccgag cagcgagtcc atgtggccgg cgctggtcgt ctgcgcgttg
     1261 ccgtactcgt cggcggtctc ggcgaagatc agggcgacgt ccagctccag ggccggcacc
     1321 gcgatcagct tctggccggc gaccgggtcg gcgaacccga ccaggtccgg ggtgatctcg
     1381 gtgtaggagg tgccgacgcc ggcgcggcag ggcatgaacg gcagcttctg cgcggcggcg
     1441 cgcaggccgg cgatgcagtg cgcctcgtcg tagtccttga aggtgaccga gccgtcctcg
     1501 accgcgcgcc ggtagaccgg ggcgacgccg gcggctccct ccatcccgac gtaggcggcg
     1561 atcagagtgt cgacgcagcc ggcggcgatc agcagctcga cgtcgagccc gccggtcggc
     1621 gcgaccacgg tcaggcccgt gaccccggag gcggccagcg cccggaccag cgccatcggg
     1681 tgcgcggcgg tgacggcgcc gccgatcccg acggtcgccc cgtccccgat ccccgccagc
     1741 gcctcctcga cggtggcgag ggcggaggtt cgctccccgg cggcgaggct catgcgatcg
     1801 tcgcctcgac cagcggtgtg accgagtcga gctcggcgat cccgcggatc gcggcttcga
     1861 gcttctccag gccggcctcg tcgaccgcca tctcggcgag caggcggcgc gagttctcgc
     1921 ggaccccgtc ccactcccag ccgtaggtgg cgccgtcggg gatcagctcg cgcacgtggc
     1981 gctcgccggt cgtcgtggtc agctcgatcc gcgccgccag ggtcggcagg cgctcgtcgg
     2041 gcagcacctc ggtgcgggcg accagctcgc ggatcaccgg gtcctcggtt tcgtgcagcc
     2101 cctccagcgt cgccgagcgg agcttgaccg ccatcgccac gcagaacggc gcgctcatca
     2161 ggctggcgcc gacgtcgcgg aacgggcccc agttgagcgt gcccgggtag ctgcggtcct
     2221 cggggttgag catgcagcgg atgctctcga cctcgtcggg gtcgacgtcg acctcggcga
     2281 ccagcgagat tgccgcctcg accgggctct gggtgatgtt gcagaccggg tagggcttgt
     2341 agatcacgtc gaggatgcgc cagcgctcgc ccagttccca ctcgccgtcg tcctcggtct
     2401 cgccggcgaa ggcgcgggcg aagccggccg ggccttcgta ccagtggcgc gcgccggccg
     2461 cgccggtggc ggcgagggtc gcagcccaga gaccgttgcg ggcggccatc ccgagctccc
     2521 agcgccactc gctggtgccc tcgatccagg tctggttgag gccgccggag aagctcgcgg
     2581 cgatcccgat cgcgctctcg gtgccctcct cgtcgagccc gagcagcgaa gcactggcgg
     2641 cggcggcccc gagcgtgccg tagagcatcg aggcgcggaa gccgcgcgcg gtgctggccg
     2701 cggcgagccg ctcgccgacc gaggcggcga cctcgtagcc ggcgatcacg gcgcgcacga
     2761 aggcctcgcc gtcggcgccg gtgcgctgcg cggtggcgag cgccgccggg atcacggccg
     2821 agccgacgtg ggtcttggcc gcgtagtgcg tgtcgtcctg cgcgcgcgcg tgcatcagcg
     2881 ccgcgttggc gaaggccgcg tgctcggcgt cgaccttggc gccgctgcag agcagcgtcg
     2941 cctcggccgg gccgaggcct tcgaccgggg gccagatcgc cggcccctcg gtgtgcgcgg
     3001 ccatcgccac gccgaagtca tgcagcaagt tgcagcggag cttctcgacc acctcggccg
     3061 ggggctcctg gtcccggacc tcgacgacga agcggccgag ccgctcgccg accgcgagct
     3121 tggtcaggtt catgatccca cctcctcaat cccacttttg atgctggcga ggaagttcgc
     3181 gccctcggca ccggtgagga cccggtggtc gcagccgagc gagaggcgga tcagccggcg
     3241 cccgtccgcg tccgcggggg ctgccgtgcc gacgccgagg atggcggcct gcggcgggtt
     3301 gatcacgggg tcgaagtcct cgatcccgta catgccgagg ttggagacgg tgaaggtggc
     3361 gtccttgagc acgtcgcggg agagccggcg cgcctccgag gcctcgacca gggcgcgcgt
     3421 ggtggcggcg atttcgttgg cgccgagccg gtcggcgtcg tagatcgcgg gcacgacgag
     3481 cccgtcgcgg acggcgatcg cgacgccgac gttgacccgc gggtgccggt gaacctcacc
     3541 gtcggcccag gaggcgttga ccaccggatt ctcgcgcagc gccttgccga cggcggcgag
     3601 catgtagtcg ttgagggtcg ggacgcggcc ggtgccctcc gccttcaggc gggtgcgctc
     3661 ggcgaagacg ccggcgacgt cgacgctgtt gcgcaggtag aagaccggga cggtggtcga
     3721 ctcggccagc cgccgcgcca tcgcgcggcg gatcatcggc agcttgacga cctcgggctc
     3781 gccctgtccg gggcgctccg ccgctgccgg ggccgtctcg ctcatcggtt cgcctactcg
     3841 tcgctgacga cggcgaacac gtggtcggcc ttgacgatgt cgaactcggc gacgtggagc
     3901 tcgacgacgg tgccgtcgat cggcgaggtg aggtccatgt tggccttgtc gctggcgatt
     3961 tccatcagcg cctcgccact ggcgaccttg gcgccgacct cgacgttgag ctggacgacc
     4021 tcgacttctt cctcggggtc cgggtcctcg atgcgaacgt ccactcgagc catgtcagct
     4081 cccctttccg gcgcactcgc ggagcgccgc ggcgatgtcg tcgacgcccg gggcgcgcgc
     4141 ttcggcgagc ggcaccgggg tcggcacgtt gatgaacggc gaagtcaccc gcaccggctc
     4201 ggtcttcagc tccgcgaggc aggactcggc ggcgatcgcg cagacctcgc tggcgatgct
     4261 gcagggcgcg gtcgcatcct gggcgacaac gacgcggccg gtcttgcgga ccgactcgac
     4321 gatcgccggg cggtcgagcg gcga
//
LOCUS       DASRIG010000473         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3886667, whole genome shotgun sequence.
ACCESSION   DASRIG010000473 DASRIG010000000
VERSION     DASRIG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886667"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2542)
                     /locus_tag="VFB52_12735"
     CDS             complement(<1..>2542)
                     /locus_tag="VFB52_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO07246.1"
                     /translation="LDLETLDGPTAIEMMESGDLTSVELTQAYIDRIYALNQAGPALN
                     AVSQLNKEALKEAKKTDELRAEGKILSPAMGLPILLKDLIDVKGMYTSAGNWSLRDSF
                     PETDSGVAKKLREHGVVILGKLGLSEYANSFGNQPSGFSNLTGQVLHATDADLNPSGS
                     SSGTGAAGAAAMSMLTIGTETSGSITSPSRAEGLVGLRPTVGLVPGYGVAPIRASQDT
                     AGPMDRTVANAALTLTAIAGSDPIDEAYYERYYEEGGLDVKDIVPEIPNPLPDYLDAL
                     DPNYVKGMRIGYFGNTAEVNEAKQALEAAGAILVERPLIETDPMPSLSGAGEQHMDID
                     FYYERLGPNAPINSLQEEIEDNEANSHEALKFGNGSHTGAAAVAYGPGTPWEAEFKTN
                     LPIRKEKAWQGIERMLNNNTPGDPSDDFTAILGTSNTGPAPNGPLAGWPAITVPLGYA
                     ATTRRPVGVSFSGTAYSEKDLLGIAYVLEQQTKKSKPASLVNPSMYRCAETVPAPAFA
                     ERGDCNPKLEETLALAGGKVPELPFALELESAQGLQDRMTAGTLTATDLTKAYLARIA
                     VANAAGPALQAVRAVNPKAVAEAEALDAERAKSGARGPLHGIPVILDDGIDAAGLPST
                     GGSIALQGATPDESSRIVAKLKAAGAIVLGKANVSELNGLFSSTMPEGYSSLGGQILL
                     ASDTDKTVAGSGAGSAAATASGMAAMAVGMETSTDTAQMIAPAAAAGVVALKPTVGRV
                     SRAGVMPIAKSQDSPGPIAKTVYDAAVQLQTIAGVDFKDTATTGTPAVGDYLTGLTGT
                     ALTGKKVAVITGGSAPYPEAVTKLGELGATTVTKTIGAAAPNPIVTRE"
BASE COUNT          308 a          815 c          938 g          481 t
ORIGIN      
        1 aactcgcggg tgacgatcgg gttcggcgcg gccgccccga tcgttttggt caccgtggtg
       61 gcgccgagtt caccgagttt cgtcaccgct tccgggtagg gagcggagcc gccggtgatc
      121 acggccactt ttttgccggt cagcgcggtc ccggtcagac cggtgaggta gtcgccgacc
      181 gccggggtgc cggtggtcgc ggtgtctttg aagtcgacac cggcgatcgt ctgcagctgg
      241 accgccgcgt cgtagaccgt cttcgcgatc gggccgggcg agtcctgcga tttcgcgatc
      301 ggcatcacgc cggcgcggct gacccggccg acggtcggct tcagcgcgac cacaccggcc
      361 gcagcggccg gggcgatcat ctgcgcggtg tcggtcgagg tctccatccc gaccgccatc
      421 gccgccatgc ccgaggcggt ggccgcggcc gagccggcgc cggaaccggc gacggtcttg
      481 tcggtgtcgg aggcgagcag gatctggccg ccgagcgagg agtagccctc cggcatggtc
      541 gagctaaaca gcccgttcag ctcggagacg ttggccttgc cgagcacgat cgcgcccgcc
      601 gccttcagtt tggcgacgat ccgcgagctt tcgtccgggg tcgctccctg cagcgcgatc
      661 gagccgccgg tgctgggcag gccggccgcg tcgatcccgt cgtcgaggat caccgggatc
      721 ccgtgcaacg ggccgcgagc gccggatttg gcgcgctctg cgtcgagcgc ttcggcctcg
      781 gcgaccgctt tcgggttgac cgcgcggacc gcctggagcg ccgggccggc cgcgttggcg
      841 accgcgatcc gggccaggta ggccttggtc aggtcggtgg cggtgagcgt gccggcggtc
      901 atccggtcct gcaggccttg cgccgattcg agctccagcg cgaagggcag ctccgggact
      961 ttgccgccgg ccagcgccag ggtctcctcg agcttcgggt tgcagtcgcc gcgctcggcg
     1021 aaggccgggg ccggcaccgt ctcggcgcag cggtacatgc tcgggttgac gaggctggcg
     1081 ggtttgctct tcttcgtctg ctgttcgagc acgtaggcga tcccgagcag gtccttctcg
     1141 ctgtaggcgg tgccgctgaa ggagacgccg accgggcggc gcgtggtcgc ggcgtagccg
     1201 agcgggacgg tgatcgccgg ccagccggcg agcgggccgt tcggggccgg gccggtgttc
     1261 gaggtgccga ggatcgcggt gaagtcgtcg ctcgggtcac cgggggtgtt gttgttcagc
     1321 atccgttcga tcccctgcca cgctttttcc ttgcggatcg gcaggttcgt tttgaattcg
     1381 gcttcccagg gcgtgccggg gccgtaggcg accgcggcgg cgccggtgtg gctgccgttg
     1441 ccgaacttga gcgcctcgtg cgagttggct tcgttgtctt cgatctcctc ctgcagggag
     1501 ttgatcggag cgttcggccc aaggcgctcg tagtagaagt cgatgtccat gtgctgctcg
     1561 ccggcgcccg agaggctcgg catcgggtcg gtttcgatca gcggccgttc gacgaggatc
     1621 gccccggcgg cttccagcgc ctgtttggct tcgttcacct cggcggtgtt gccgaagtag
     1681 ccgatccgca tgcctttgac gtagttcggg tcgagcgcgt cgaggtagtc gggcagcggg
     1741 ttgggaatct ccgggacgat gtctttgacg tcgaggccgc cttcttcgta gtagcgctcg
     1801 taatacgctt cgtcgatcgg gtccgagcct gcgatcgcgg tcagggtcag ggccgcgttg
     1861 gcgaccgtgc ggtccatcgg gccggcggtg tcctgggagg cgcggatcgg ggcgacgccg
     1921 tagccgggca ccaacccgac cgttggccgc agcccgacca ggccctcggc ccgcgagggc
     1981 gaggtgatcg agccggaggt ctcggtaccg atcgtcagca tcgacatcgc cgcggcgccg
     2041 gcggcgccgg taccggagga ggagccgctc gggttgaggt cggcgtcggt cgcgtgcagg
     2101 acctggccgg tcaggttgga gaagcccgag ggctggttgc cgaagctgtt cgcgtactcg
     2161 gagaggccga gcttgccgag gatcacgacc ccgtgctcac gcagcttttt cgccaccccg
     2221 gagtcggttt ccgggaacga gtcgcgcagc gaccagttac cggccgaggt gtacatccct
     2281 ttcacgtcga tcagatcctt gagcaggatc ggcagcccca tcgccgggct gaggattttg
     2341 ccttccgccc gcagttcgtc cgtcttcttc gcttctttca gcgcttcttt gttgagctgg
     2401 gagaccgcgt tcagcgccgg gccggcctgg ttcagcgcgt agatccggtc gatgtaggcc
     2461 tgggtcagtt caaccgaggt cagatccccg ctttccatca tctcgatggc ggtcgggccg
     2521 tcgagggtct cgaggtcgag ct
//
LOCUS       DASRIG010000474         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_228616, whole genome shotgun sequence.
ACCESSION   DASRIG010000474 DASRIG010000000
VERSION     DASRIG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228616"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..813)
                     /locus_tag="VFB52_12740"
     CDS             complement(100..813)
                     /locus_tag="VFB52_12740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZO07247.1"
                     /translation="MARRSSPSNSLLGDSGLSEETLEDLVSHAAGGEARPVDVVLRGL
                     DVLISASVLFVLSPLLAVVALAILLTSGRPVLYRGARVGRAGHLFWMYKFRTLKPDAE
                     DRLGPYYGEELTRRTEEERTLIGGVLRSTHLDEVPQLWNVLRGDMAIVGPRPIRPAFF
                     AELCREIPQYWQRLVVRPGVTGFAQTRITREESWSNKLAHDMEYIADRSVGLYFNVLF
                     ATVGKVMGKGPAAPGNQET"
     gene            complement(777..1823)
                     /locus_tag="VFB52_12745"
     CDS             complement(777..1823)
                     /locus_tag="VFB52_12745"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HZO07248.1"
                     /translation="MKIWVDMSAPAHVLVLRPIIQRLQAEGHEVEVTSRDYAQTQQLL
                     ELHGMPHTPIGQHGGASRVGKAYRLGERTARMLAYGRGRGFDLALAHGSNDLALAARA
                     LGVPEANMHDYEYAVTQHHIGCRLARRVMFPDSVPASRLRRFGVTPEKLFPYPGLKEE
                     YYLYDFEPDPEALTRLGVETERVVVIVRPPPDVSLYHRKSNPLFPKVLERIGRDDGVH
                     AVVLPRTEAQRSFINGLALPSLIVPDQAVEAQSLVALADLVISAGGTMNREAAALGTP
                     VYTTYGGRLGGVDEALIRSGRLRPLTDQRAVELEKRSAAATATKRDPAPLVETILGTV
                     GAKSRGEAPQVTPS"
     gene            complement(1828..3102)
                     /gene="glyA"
                     /locus_tag="VFB52_12750"
     CDS             complement(1828..3102)
                     /gene="glyA"
                     /locus_tag="VFB52_12750"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZO07249.1"
                     /translation="MSELPENFQTAPLNEVDPEIAAVLEGELRRQRNTLEMIASENFV
                     PQAVLEAVGSVLTNKYAEGYPGRRYYGGCEEVDVAEQLAIDRAKELFGAEHANVQAHS
                     GAQANNAAYMALIEPGDTFLGMALDHGGHLSHGMKINVSGKLYNPVPYHVSRDDFRVD
                     MDEVAALAQEHRPALIVAGWSAYPRQLDFAAFREIADSVGAKLMVDMAHFAGLVAAGE
                     HPNPVEYADVVTTTIHKTLCGPRSGMILCREEHAKAIDKAIFPGQQGGPLMHTIAAKA
                     VALHVAASPQFRRRQRQTIANARELATGLEAGGVPVLTGGTDVHLVLVDLTPTGLDGQ
                     TAEDRLDEVGITVNRNAIPFDPRPPMNPSGLRIGTPALTTRGMGEADMAEIASVIATA
                     LTGDFVFEKDGLRARTEALMGRHPLYPELATA"
     gene            3145..3915
                     /locus_tag="VFB52_12755"
     CDS             3145..3915
                     /locus_tag="VFB52_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07250.1"
                     /translation="MRPPIPVLAAVFLLAAALPMTAQAKPDNPCDGKEAAQLLCPNLR
                     IGPASEIYVENRGPGQVWLRATSDVRSRGRGPMELVGFRNGWRTMRTNQRIWKVDGGF
                     KTVKSEATLRFTDVGYQFGGAYWKVHELARFDLRPVEADGTVGEPVRTSPKLNYCLRD
                     LYRTQPSAVSPPSRYYPACNQNPYRNRVTLGTSVGWSDIYPAPYHQQYINVDGLSGCF
                     VYEMRVDPQNYLFESNEEDNRSRRLVRLPYTGPVTSCP"
     gene            complement(3920..>4593)
                     /locus_tag="VFB52_12760"
     CDS             complement(3920..>4593)
                     /locus_tag="VFB52_12760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZO07251.1"
                     /translation="RQSGQKGTSAVGERLSLERDGAEAVRAALEATIARGGTVVFPAD
                     GLYGLACDPLDEGAIQRIHALKGRDDGKPSAVMYFSPLAMRELVAGLGPRTKAAVSAL
                     LPGPLTLVVANPERRYPLACREDVGRLGVRLIGGPLAGAMVPIFQTSANLSGEPAPAR
                     FEQVPASIADGVDLAIEGGELTGLPSTVVDIAAIDDDGSWRILREGAVSAGDLASALA
                     AFGVD"
BASE COUNT          674 a         1581 c         1643 g          695 t
ORIGIN      
        1 ccgcggatag cgcggttaag tcccaccgga accgcgacac cggggcatct cgcacaccta
       61 cccagcccgt gggtgtgcgt gatggcccgg tgtccttagc tacgtttcct ggttccccgg
      121 cgccgccggg cctttgccca tcaccttgcc gaccgtggcg aagaggacgt tgaagtaaag
      181 ccccaccgag cggtcggcga tgtactccat gtcgtgggcg agcttgttcg accaggattc
      241 ctcgcgggtg atccgggtct gggcgaagcc ggttacgccg gggcggacga cgaggcgttg
      301 ccagtactgg gggatctcgc ggcagagctc ggcgaagaag gcggggcgga tcgggcgcgg
      361 gccgacgatc gccatatcgc cgcgcagcac gttccagagc tgtggcacct cgtcgagatg
      421 ggtcgagcgc aggacgccgc cgatcagcgt ccgctcttcc tccgtgcgcc gggtcagctc
      481 ctcgccgtag tagggcccga gccggtcttc ggcgtccggc ttcagggtgc ggaacttgta
      541 catccagaag aggtggccgg cgcggccgac gcgggcgccg cggtagagaa ccgggcggcc
      601 cgaggtaagc aggatcgcca gcgcgacgac cgccaagagc ggcgaaagca caaacaggac
      661 cgaggcggag atcagtacgt cgagcccgcg cagaacgacg tcaaccggcc gcgcttcgcc
      721 acccgcggca tgcgagacga ggtcttccag ggtctcttca ctgagcccgg agtcgcctag
      781 gagggagtta cttggggagc ttcgccgcgc gatttggctc cgacggtacc gagaatcgtc
      841 tctaccaagg gggcgggatc gcgttttgtc gcggtcgctg cggcactgcg cttctccaac
      901 tcgaccgccc gctggtcggt cagcgggcgc aggcggccgg agcggatcag cgcttcgtcg
      961 acgccgccga gccgcccgcc gtaggtcgtg tagacgggag tgccgagcgc cgcggcctcc
     1021 cggttcatcg tgccgccggc cgagatcacc aggtcggcga gcgcgaccag gctctgcgcc
     1081 tcaaccgcct ggtcggggac gatcagcgag ggcagcgcga gcccgttgat gaagctgcgc
     1141 tgggcttcgg tgcgcggcag gacgacggcg tgcacgccgt cgtcgcggcc gatccgctcc
     1201 agcactttcg ggaaaagcgg gttcgacttg cggtggtaga gcgagacgtc gggcggcgga
     1261 cgcacgatca cgacgacgcg ctcggtctcg accccgagtc gggtcagcgc ctcggggtcg
     1321 ggctcgaagt cgtagaggta gtactcctcc ttcagccccg ggtaggggaa gagcttctcc
     1381 ggggtgacgc cgaaacggcg caggcgcgag gccgggaccg agtcggggaa catcacccgg
     1441 cgggcgagcc ggcagccgat gtggtgctgg gtgacggcgt actcgtagtc gtgcatgttg
     1501 gcctcgggca cgccgagcgc gcgggcggcg agcgcgaggt cgttggagcc gtgggcgagc
     1561 gccaggtcga agccgcggcc gcggccgtag gcgagcatcc gcgccgtgcg ctcaccgagc
     1621 cggtaggcct tgccgacccg ggaggcgccg ccgtgctggc cgatcggcgt gtgcggcatc
     1681 ccgtgcagct cgagcagctg ctgggtctgg gcgtagtcgc gcgaggtcac ctcgacctcg
     1741 tggccttcgg cctgcaggcg ctggatgatc gggcgcagca cgaggacgtg cgccggggcg
     1801 ctcatgtcga cccagatctt caaacggtca ggcggtcgcc agctcggggt agagcgggtg
     1861 gcgccccatc agcgcttcgg tgcgggcgcg cagcccgtcc ttctcgaaga cgaagtcgcc
     1921 ggtcagcgcg gtcgcgatca ccgaggcgat ttcggccatg tccgcctctc ccatcccgcg
     1981 cgtggtcagc gccggggtgc cgatccgcag gcccgacggg ttcatcggcg ggcgcgggtc
     2041 aaacgggatc gcgttgcggt tgaccgtgat cccgacctcg tccaggcggt cctcggcggt
     2101 ctggccgtcg agcccggtcg gggtcaggtc gaccagcacc aggtggacgt cggtgccgcc
     2161 ggtcagcacc ggcacgccgc cggcttcgag gccggtggcg agctcgcgcg cgttggcgat
     2221 cgtttgccgc tggcggcggc ggaactgcgg gctggcggcg acgtgcaggg cgacggcctt
     2281 ggcggcgatc gtgtgcatca gcggcccgcc ctgctggccc gggaagatcg ctttgtcgat
     2341 cgctttcgcg tgttcctccc ggcagaggat catgccgctg cgcggtccgc agagcgtctt
     2401 gtggatcgtc gtcgtgacca cgtcggcgta ctcgaccggg ttcggatgct cgccggcggc
     2461 gacgaggccg gcgaagtggg ccatgtcgac catcagcttc gcccccaccg agtcggcgat
     2521 ctcgcggaag gcggcgaagt cgagctggcg cgggtaggcg gaccagccgg cgacgatcag
     2581 cgccgggcgg tgctcctgcg cgagcgccgc gacctcgtcc atgtcgacgc gaaaatcgtc
     2641 gcggctgacg tggtacggga ccgggttgta gagcttgccg gagacgttga ttttcatccc
     2701 gtggctgagg tggccgccgt ggtcgagcgc catgccgagg aaggtgtcgc ccggttcgat
     2761 cagcgccatg taggcggcgt tgttggcctg ggcaccggag tgagcctgga cgttggcgtg
     2821 ctcggccccg aacagctcct tggcgcggtc gatcgccagc tgctcggcga cgtcgacctc
     2881 ctcgcagccg ccgtagtagc ggcgaccggg gtagccctcg gcgtacttgt tggtcagcac
     2941 cgagccgacc gcttccagga ccgcctgggg cacgaagttc tccgaggcga tcatctccag
     3001 cgtgttgcgc tggcgccgca gctcgccctc gagcacggcg gcgatctcgg gatcgacctc
     3061 gttgaggggc gcggtttgga agttctcggg cagctcgctc atcgtcggcc gacgatagca
     3121 acgcatcggg atagtctccg gccggtgaga ccccccattc ccgtcttggc cgcggtgttc
     3181 ctgcttgccg ctgcgctccc gatgacggca caggcaaaac cggataaccc ctgcgacggc
     3241 aaggaagccg cgcagctgct ctgcccgaac ttgcggatcg ggccggcgag cgagatctat
     3301 gtcgagaacc gggggccggg tcaggtctgg ctgcgggcga ccagcgacgt gcgcagccgc
     3361 ggccgcggcc cgatggagct ggtcgggttc cgcaacggtt ggcggacgat gcggaccaac
     3421 cagcggatct ggaaagtcga cggcggattc aagacggtga agagcgaagc caccctgcgc
     3481 ttcaccgacg tcggctacca gttcggcggc gcctactgga aggtccacga actcgcccgc
     3541 ttcgacctcc gcccggtcga agccgacggc acggtcggcg aaccggtccg caccagccca
     3601 aagctcaact actgcctgcg tgacctctac cggacccagc ccagcgcggt ctcgccgccg
     3661 agccgctact accccgcctg caaccagaac ccgtaccgca accgggtcac cctggggacc
     3721 tcggtcggct ggtcggacat ctaccccgcg ccctaccacc agcaatacat caacgtcgac
     3781 gggctcagcg gctgcttcgt ctacgagatg cgggtcgacc cacagaacta cctgttcgag
     3841 tcgaacgagg aagacaaccg ctcgcgccgc ctcgtccggc tcccctacac cggccccgtc
     3901 accagctgcc cgtagcgccc tagtcgacgc caaaggcggc gagggcgctg gccaggtcgc
     3961 ctgcggagac cgcgccttcg cgcaggatcc gccaggagcc gtcgtcgtcg atcgcggcga
     4021 tgtcgacgac ggtcgaaggc agcccggtca gctcgccgcc ttcgatcgcg agatcgaccc
     4081 cgtcggcgat gctcgccggc acctgctcga agcgagccgg cgccggctcg ccgctgaggt
     4141 tggccgaggt ctggaagatc ggcaccatcg cccccgccag cggcccgccg atcaggcgca
     4201 cgccgagccg cccgacgtcc tcgcgacagg cgagtgggta gcgccgctcg gggttggcga
     4261 cgaccagggt cagcggcccc ggcagcagcg cgctgaccgc cgctttcgtg cgcggcccca
     4321 ggcccgcgac cagctcccgc atcgccagtg gcgagaagta catgaccgcc gagggcttgc
     4381 cgtcgtcgcg ccccttgagc gcgtggatgc gctggatcgc gccctcgtcg agcgggtcac
     4441 aggcgagccc gtagagcccg tcggccggaa acaccacggt cccgcctctc gcgatcgtcg
     4501 cctccagcgc agcccgcacc gcctccgccc catcccgctc caacgaaagc cgctcaccca
     4561 ctgcgctcgt ccccttttgc ccgctctggc gtg
//
LOCUS       DASRIG010000475         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1269208, whole genome shotgun sequence.
ACCESSION   DASRIG010000475 DASRIG010000000
VERSION     DASRIG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269208"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFB52_12765"
     CDS             <1..1333
                     /locus_tag="VFB52_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO07252.1"
                     /translation="ANDFTAVNVALPTMEEDFHTDITTIQWVINAYALTFGVMIVTGG
                     RLADMFGRRNAFFLGSAIFATMSLLGGVAQSEAWLIAARTLMGIGGALMWPAILGMTY
                     ELLPEEKAGLAGGIIIGAAGLGNAVGPLLGGVLTDELSWRWIFYLNVPIAIFAVGVTY
                     LLVHVKEPESSERRIDYAGIATLSVGLVSLLVALDQAADWGWGDPRVIAMLALAAILI
                     AAFLPIERRAGEHALVPQKVMRNESFTAACFAVLFMAGTFFAALLYLPQLMEKQLGYS
                     ALESGVGMLPFLAVFALSSFAAGPLYNRVGPKVLAVVGAALIAAGPLLFFLMIEHDSG
                     YGALVPGMIVLGIGAGSFVPTATTAGVTSVDESQTSLAGGIVYMFQIAGGSIGLGLTT
                     TVFASQVPPFTDGVHAAFQLDAALSLIGLAIALLFVGGRLFRRRQPVGEAA"
     gene            complement(1344..1895)
                     /locus_tag="VFB52_12770"
     CDS             complement(1344..1895)
                     /locus_tag="VFB52_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07253.1"
                     /translation="MKRALLITIAALVACSATVAHGAERSFNVLLTGGDEVNSISVKL
                     STDGRSYLIDSAFGPLEAPSGICEHREGSENSLICEATAIASFEVNAGGGNDHINFSP
                     KVPVPVTLRGGPGDDRLKGGSASDKLVGGAGNDKLYGRAGDDWLFGGPGNDVLYGSFG
                     DDRLLGGPGVNEIFGGSGTNETN"
     gene            complement(1935..2966)
                     /locus_tag="VFB52_12775"
     CDS             complement(1935..2966)
                     /locus_tag="VFB52_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HZO07254.1"
                     /translation="MKAVVLTKTGGPEVLQVQERPDPVVAPGQIRIAVRAAGINFADT
                     LARVGLYPDAPKPPCVLGYEVAGEVETVGEGVTEFKVGDRVFAGTRFNGQAELVSVDA
                     GQALPLPDALSFEQGAAFPVNYGTAYAALVLMGGMRSGDRVLIHAAGGGVGISATQLA
                     RNAGAEVFGTASPAKHDAIRAQGVTHAIDYRNQDFEDEVMRITGGEGVDLVMDALGPT
                     SFRKDYRLLRSGGRLVMYGLSENSDGGVRNIPATLKSLAKMPWATMPWWKSLMMMNEN
                     KGVFGLNMLSWWEREGNLDRVTEPLMADLEAGRIEPVVAEAFPFERAGEAHQFIAERR
                     NVGKVLLLP"
     gene            complement(3014..4597)
                     /gene="serA"
                     /locus_tag="VFB52_12780"
     CDS             complement(3014..4597)
                     /gene="serA"
                     /locus_tag="VFB52_12780"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZO07255.1"
                     /translation="MRVLVKEKIADTGVDLLRSKFEVDLGLEMSDEELAEKIGEYDAI
                     LIRSATKLTPELIDRADNLKVIGRAGTGVDNVDIPAATRRGIIVANAPESNSVAAAEH
                     TLALALALFRNVPQAHASLVEGRWDRAKFKGAELYGKTLGVIGFGRIGQLVAKRAQSF
                     EMEVLAFDKFVSAERFRELGVEGVESIDELLGRADLVTLHVPKTPETIGLINAEAIAK
                     MKDGARVVNCARGELVDLDDLMAGLESGKLGGAALDVFPSEPFTEHPIFARGDVVVTP
                     HLGASTAEAQDRAGVVTAEQVTEALTGGVVTNAVNIAAVRPEAMEALAPFVPLCEKLG
                     RLAQGLGDGSVDGVTAEFRGRIASHDTRLLGIAVLVGILSGNTEEQVNLVNAPQMAEE
                     RGIELIETKDSSAEDFTELVTVRVRSGDGEVEVAGTSVGPRNEPYLVSAWGESFYLPF
                     AEHITVFRYADQPGMIGRVGTAFGEEGVNIVSAAVGAEAAGGESAVMALTTDAAVRPE
                     ALERILGLDGFAVGRSVDL"
     gene            complement(4594..>5249)
                     /locus_tag="VFB52_12785"
     CDS             complement(4594..>5249)
                     /locus_tag="VFB52_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZO07256.1"
                     /translation="EGAGDSLVLIDATSGAGGLPVEIAQADAYYFAPQKAFGADGGLW
                     LAVLSPAAIARIEELDGAGDRWQPEFLSLATALDNSRKQQTYNTPALASLILLGDTVE
                     WMLANGGLDWCVRRTTDSSSRLYGWAEESEFASPFVADPEKRSLVVGTIDFSDSIDGK
                     ALAAALRANGIVDVEPYRKLGRNQLRIGMFPAVEPDDVAALTACIDWVIENVDGVAK"
BASE COUNT          737 a         1791 c         1860 g          861 t
ORIGIN      
        1 cgccaacgac ttcacggcgg tcaacgtcgc cctgccgacg atggaggagg acttccatac
       61 cgacatcacc acgatccagt gggtgatcaa tgcctacgcg ctcaccttcg gggtgatgat
      121 cgtgaccggc ggccggctgg ccgacatgtt cgggcggcgc aacgcgttct tcctcggcag
      181 cgcgatcttc gcgacgatgt cgctgctcgg cggggtcgcg cagagcgagg cctggctgat
      241 cgcggcgcgc acgctgatgg ggatcggcgg ggcgctgatg tggccggcga tcctcgggat
      301 gacctatgag ctgctgccgg aagagaaggc cgggctggcc ggcgggatca tcatcggcgc
      361 cgccggactg ggcaatgcgg tcggcccgct gctcggcggt gtgctcaccg acgagctgag
      421 ctggcgctgg atcttctatc tgaacgtgcc gatcgcgatc ttcgcggtcg gggtcaccta
      481 cctgctcgtc cacgtcaagg agccggaatc gagtgagcgg cgaatcgact acgcggggat
      541 cgcgacgctc tcggtcggcc tcgtctcgct gctggtggcg ctcgaccagg cagccgactg
      601 gggctggggc gacccgcggg tgatcgcgat gctcgccctg gcggcgatcc tgatcgcggc
      661 cttcctgccg atcgagcgac gcgccggtga gcacgccctg gttccgcaga aggtgatgcg
      721 caacgagagc ttcaccgccg cctgcttcgc ggtcctgttc atggccggga ccttcttcgc
      781 cgcgctgctc tatctgccgc agctgatgga gaaacagctc ggctactcgg cgctcgaatc
      841 gggcgtcggg atgctgccct tccttgccgt cttcgcgctc tcctcgttcg ccgccgggcc
      901 gctctacaac cgggtcgggc cgaaggtgct ggcggtggtc ggggccgccc tgatcgccgc
      961 cgggccgctg ctcttcttcc tgatgatcga gcacgactcc ggctacggcg ccctcgtccc
     1021 ggggatgatc gtgctcggga tcggcgccgg cagcttcgtc cccaccgcga ccaccgccgg
     1081 ggtgacctcg gtcgacgagt cgcagaccag cctcgccggc gggatcgtct acatgttcca
     1141 gatcgccggc ggctcgatcg gcctcggcct gaccacgacc gtgttcgcct cccaggtccc
     1201 gcctttcacc gacggggtgc acgccgcgtt ccagctcgac gcggcgctct cgctgatcgg
     1261 gctcgcgatc gccctgctgt tcgtcggcgg tcggctgttc cggcgccggc aaccagtcgg
     1321 ggaagcggcc tgagccccga ccgctagttg gtttcgttgg tgccgctgcc gccgaagatt
     1381 tcgttgacgc ccgggccacc gagcaggcgg tcgtcgccga agcttccgta gaggacgtcg
     1441 ttgccggggc cgccgaacag ccagtcgtcg ccggcgcggc cgtagagctt gtcgttgccg
     1501 gcgccgccga ccagcttgtc ggaggcggag ccgcccttca ggcggtcgtc gccggggccg
     1561 ccgcgcaggg tgaccggaac cgggaccttc ggcgagaagt tgatgtggtc gttgccgccg
     1621 ccggcgttga cttcgaagct ggcgatcgcg gtcgcttcgc agatcagcga gttttcgctg
     1681 ccttcgcggt gctcgcagat cccgctcggg gcttccagcg gcccgaaggc tgagtcgatc
     1741 aggtaggagc ggccgtcggt gctgagcttg acgctgatcg agttcacctc atcgccgccc
     1801 gtgagcagga cgttgaagga gcgttcagcg ccatgcgcaa ccgtggcgga gcaggccacc
     1861 agcgcggcga tcgtgatcag caaggccctt ttcatggcgg agtctttcgg cccggatcgc
     1921 ccggaccctc tcgcctatgg caacaacagg accttgccca cgttgcggcg ctcggcgatg
     1981 aattgatggg cctcgccggc ccgctcgaag ggaaatgcct cggcgacgac cggctcgatc
     2041 cggcccgctt cgaggtcggc catcaacggc tcggtgacgc ggtcgaggtt gccctcgcgt
     2101 tcccaccagg acagcatgtt gagcccgaag acgcctttgt tctcgttcat catcatcagg
     2161 cttttccacc agggcatcgt cgcccagggc atcttcgcca ggctcttcag cgtcgccggg
     2221 atgttgcgca cgccgccgtc ggaattctcc gagagcccgt acatgaccag ccggccgccg
     2281 gagcgcagca ggcggtagtc cttgcggaac gaggtcgggc cgagcgcgtc catgaccagg
     2341 tcgacgccct cgccgccggt gatccgcatc acctcgtcct cgaagtcctg gttgcggtag
     2401 tcgatcgcgt gggtgacgcc ctgggcgcgg atcgcgtcgt gcttggccgg cgaggcggtg
     2461 ccgaagacct cggcgccggc gttgcgggcg agctgggtgg cggagatccc gacgccgcca
     2521 ccggcggcgt ggatcagcac gcggtcgccg gaccgcatcc cgcccatcag caccagcgcc
     2581 gcgtaagcgg tcccgtagtt gaccgggaag gcggcgccct gctcgaagct gagcgcgtcg
     2641 gggagcggca gcgcctggcc ggcgtcgacg ctgaccagct cggcctgccc gttgaagcgg
     2701 gtgccggcga agacgcggtc gccgactttg aactcggtca ccccctcccc cacggtctcg
     2761 acctcaccgg cgacctcgta gccgagcacg cagggcggct tcggggcgtc cggatagagg
     2821 ccgacccggg cgagggtgtc agcgaagttg atcccggccg cgcgcacggc gatgcggatc
     2881 tggccaggcg cgacgacggg atccggtcgc tcctgcacct ggagcacctc gggcccgccg
     2941 gtcttggtca gcactacggc tttcacacgc gcagcctaac ccggcgaaga gaaacgcggg
     3001 aattcctgag tgactagagg tcgaccgagc ggccgaccgc gaagccgtcc aagccaagaa
     3061 ttcgctcgag cgcctcgggg cggacggcgg catccgtcgt cagcgccatc acggcgctct
     3121 cgccgcctgc cgcctcggcg ccgaccgcgg cggagacgat gttgacgccc tcctcgccga
     3181 aggcggtacc gacgcggccg atcatccccg gctggtcggc gtagcggaag acggtgatgt
     3241 gctcggcgaa gggcaggtag aagctctcgc cccaggcgga gaccaggtag ggctcgttgc
     3301 gcgggccgac gctggtgccg gcgacctcaa cttcgccatc gccggagcgg acccgcacgg
     3361 tgaccagctc ggtgaagtcc tcggcgctgg agtccttggt ctcgatcagc tcgatccccc
     3421 gctcctcggc catctgcggc gcgttgacca ggttgacctg ctcctcggtg ttgccggaga
     3481 ggatgccgac caggacggcg atcccgagca ggcgggtgtc gtgggaggcg atgcggccgc
     3541 ggaactcggc ggtgacgccg tcgaccgagc cgtcccccaa tccctgggca agccgcccca
     3601 atttttcgca gagcggcacg aacggggcca gcgcctccat cgcctccggg cgcacggcgg
     3661 cgatgttgac cgcgttggtg acgacgccgc cggtcagcgc ctcggtcacc tgctcggcgg
     3721 tgacgacgcc ggcgcggtcc tgagcctccg cggtcgaggc gccgaggtgc ggggtgacga
     3781 cgacgtcgcc gcgggcgaag atcgggtgct cggtgaacgg ctccgagggg aagacgtcga
     3841 gcgcggcgcc gcccagcttg ccggactcga ggccggccat caggtcgtcg aggtcgacca
     3901 gctcgccgcg ggcgcagttg acgacgcggg cgccgtcctt catcttcgcg atcgcctcgg
     3961 cgttgatcag gccgatcgtc tccggcgtct tcggcacgtg cagggtgacg aggtcggcgc
     4021 ggccgagcag ctcgtcgatg ctctcgaccc cctcgacccc tagctcgcgg aagcgctcgg
     4081 cggagacgaa cttgtcgaag gcgaggacct ccatctcgaa gctctgggcg cgtttggcga
     4141 cgagctgtcc gatgcggccg aagccgatca ccccgagcgt cttgccgtag agctcggcgc
     4201 ccttgaactt ggcccggtcc cagcggccct cgaccagcga ggcgtgcgcc tgcgggacgt
     4261 tgcggaacag cgccagggcg agtgccaggg tgtgctcggc ggcggcgacg gagttcgatt
     4321 cgggcgcgtt ggcgacgatg atcccgcgcc gggtcgcggc cgggatgtcg acgttgtcga
     4381 caccggtgcc ggcgcggccg atcaccttga ggttgtcggc gcggtcgatc agctccggag
     4441 tcagcttcgt ggcgctgcgg atcaggatcg cgtcgtactc gccgatcttc tccgccagct
     4501 cctcgtcgga catctcgagg ccgaggtcga cttcgaattt cgagcgcagc aggtcgacgc
     4561 cggtgtcggc gatcttctcc ttgaccagga ccctcacttg gccaccccgt ccacgttctc
     4621 gatcacccag tcgatgcagg cggtgagcgc ggcgacgtcg tcgggctcaa ccgcggggaa
     4681 catcccgatc cgcagctggt tgcggccgag cttgcggtag ggctcgacgt cgacgatccc
     4741 gttggcgcgc agggcggcgg cgagggcctt gccgtcgatg gaatcagaaa agtcgatagt
     4801 gccgacgacg agggaacgct tctcgggatc ggccacgaac gggctcgcaa actcgctctc
     4861 ctccgcccag ccgtatagac gactcgatga gtcggtcgtt cgacgcacac accagtcgag
     4921 gccgccgttg gcgagcatcc actcaaccgt gtcgccgagc aggatcaggc tcgccagggc
     4981 gggcgtgttg taggtctgct gcttgcgcga gttgtcgagc gcggtcgcca gcgagaggaa
     5041 ttcgggctgc cagcggtcac cggcgccgtc gagctcctcg atccgggcga tcgccgccgg
     5101 gctgagcacg gccagccaga gcccgccgtc ggcgccgaag gccttctgcg gggcgaagta
     5161 gtaggcgtcg gcctgggcga tctcgacggg caggccgcca gcgcccgagg tggcgtcgat
     5221 caggaccagc gagtcgccgg cgccctcgg
//
LOCUS       DASRIG010000476         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_984231, whole genome shotgun sequence.
ACCESSION   DASRIG010000476 DASRIG010000000
VERSION     DASRIG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984231"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFB52_12790"
     CDS             complement(<1..534)
                     /locus_tag="VFB52_12790"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07257.1"
                     /translation="MKSERKAQGFDVYEEQRQTGLRLEILRLLVLAVFVALGVRLWWL
                     QVMNHEVFAGQAESNRMRVIPIPARRGTIYDRQGRELVTSRISFNIVVSRTDVANFAE
                     LTDLLTANLEIDRAWIEKRFEEAKYEPRWESIVVKEMASPAEVAWVRAHRYEHPEVRA
                     EEAPQRLYLHGALAAHAL"
     gene            complement(705..1226)
                     /gene="mreD"
                     /locus_tag="VFB52_12795"
     CDS             complement(705..1226)
                     /gene="mreD"
                     /locus_tag="VFB52_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZO07258.1"
                     /translation="MAVCLLAAAFLQTTLSRYNPWLGYIDWLLLVAVYASSLRDPVLA
                     LLTATAAGALYDSLSGGVPIGVNGIAKVLATYLAYWVSSKIVAENLLVSILNVAGASV
                     VNTLVSLAFYRMLKFTYLPPLSGGNATLAAVAMGVLANLTVSIPLYFLLDRVFKGAGL
                     RSRRSEAMRMRRR"
     gene            complement(1300..2229)
                     /gene="mreC"
                     /locus_tag="VFB52_12800"
     CDS             complement(1300..2229)
                     /gene="mreC"
                     /locus_tag="VFB52_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZO07259.1"
                     /translation="MSFKIKTKKNPTVLLAILLVAHMGVISLNRVQGLSARGDERYAQ
                     VWLMAALAPFQWATAKTVGTISGLWHNYFSLRDSRLENDRLRAELAERGADLTRISDE
                     LKLAGQAQALSEWQAAQKAPTVAARVIARDADQWFNTVVIDRGTLAGVAKDHPVVTPE
                     GLVGRVVDAAPNAARVLLMTDERHGAGAIIGQLAESRLLGVVEGRNHQLCEMKIVSTL
                     EKVEPGETVITSGQDGIYPRGLIIGRVRRIEGGAEASPLAVEVEPAAPLAKLDLVAVL
                     KVSKAQTRGPVDQLLDQEKERERQTKPARSRRR"
     gene            complement(2333..>3340)
                     /locus_tag="VFB52_12805"
     CDS             complement(2333..>3340)
                     /locus_tag="VFB52_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZO07260.1"
                     /translation="DLAIDLGTANTLVFARGRGIVVSEPSIVAINKVSHKVEAVGKEA
                     KEMLGRTPGNIVAIRPMKDGVIADFEVTEKMLQYFIQKAHNGKQWVSPRIVIGIPSEI
                     TQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPHGNMIVDIGGGTTDIAVI
                     SMSGIVYSKSVRVAGNEMDEAITQYIKRKYNLLIGERTAESIKIEIGSAAPVDEPMTM
                     EIKGRNLIEGIPKTIVVSDEEIREALADSVATIINAVRVALERTPPELSADIVDRGIV
                     LTGGGAMLKKLDVRLMQETGLPVSMAEDPLSSVVLGAGTMLSDFDLLRRVRWDGTTLP
                     S"
BASE COUNT          524 a         1160 c         1094 g          562 t
ORIGIN      
        1 gagggcgtgc gcggcgagcg cgccgtgaag gtagaggcgc tgcggcgcct cctcggcgcg
       61 cacctcgggg tgctcgtagc ggtgcgcccg gacccaggcg acctcggcgg gcgaggccat
      121 ctccttgacg acgatcgact cccagcgcgg ctcgtacttg gcctcctcga aacgcttctc
      181 gatccaggcg cggtcgatct ccaggttcgc cgtcaggagg tcagtcagct cggcgaaatt
      241 cgccacgtcc gtgcggctga cgacgatgtt gaaggagatg cggctcgtca ccagctcgcg
      301 gccctgccgg tcgtagatcg tcccgcggcg ggccgggatc gggatgaccc gcatgcggtt
      361 ggactcggcc tgcccggcga agacctcgtg gttcatgacc tggagccacc agagccggac
      421 gccgagcgcc acgaagacgg cgagcaccag cagccggagg atctccagcc ggagccccgt
      481 ctgccgctgc tcctcataga cgtcaaagcc ctgcgctttc cgctccgatt tcataaggcc
      541 tacccccggc cgctccgcgt gccggcccca agtatagcac tatcagcgat cagcggtcag
      601 ctctcagccg tcagccggaa acctttgtcg cgggactgcc atgtcgacca ccggggtgag
      661 gctccaccgc tgtaaagctg atcgctcaga cccgacccgc gcccttaacg ccgccgcatg
      721 cgcatcgcct cactccgccg cgaccgcagc cccgctccct tgaagacccg gtcgagcagg
      781 aagtagagcg ggatcgagac cgtgaggttg gccaagaccc ccatggcaac cgccgccagc
      841 gtcgcgttgc ccccggagag cggcggcagg taagtgaact tgagcatgcg gtaaaaggcc
      901 aggctcacca gcgtgttgac gacgctcgcc ccggccacgt tgaggatgct caccagcagg
      961 ttctcagcca cgatcttgga cgagacccag taggccaggt aggtggccaa caccttggcg
     1021 atgccgttga ccccgatcgg caccccgccc gagagcgagt cgtagagcgc gccggcggcg
     1081 gtcgccgtca gcagcgcgag caccgggtcg cgcagcgagc tggcgtagac cgcgacgagc
     1141 agcagccagt cgatgtaccc gagccacgga ttatatctgc tcagcgtggt ctggaggaag
     1201 gcggccgcca gcaagcagac agccattttc ccgagccgcg cccgaccttg tatctctttt
     1261 tccaagccgc ctttcgcccc cgctgcccga cgcccggcct caccgccgcc gcgagcgggc
     1321 cggcttcgtc tgtctctccc gctccttctc ctggtcgagc aactggtcga ccgggccgcg
     1381 ggtctgcgcc ttcgagactt tgaggacggc aaccaggtcg agtttggcga gcggggctgc
     1441 cggctcgacc tcgacggcca gagggctcgc ctcggccccg ccctcgatcc gcctgacgcg
     1501 gccgatgatc aggccccgcg ggtagatgcc gtcctgcccc gaagtgatca ctgtctcgcc
     1561 cggctcgacc ttctcgagcg tcgagacaat cttcatctcg cagagctgat ggtttctccc
     1621 ctcgaccacg cccaggagcc gcgactcggc cagctgcccg atgatcgccc ccgcaccatg
     1681 ccgctcgtcg gtcatgagga gcacccgcgc ggcgttcggg gccgcgtcga cgacgcgccc
     1741 gacgaggccc tcgggcgtga ccacggggtg gtccttggcg acgcccgcga gcgtgccgcg
     1801 gtcaatgacc acggtgttga accactggtc ggcatcccgc gcgatgaccc gcgcggcgac
     1861 cgtcggggcc ttctgcgccg cctgccactc ggagagcgcc tgcgcctgcc ccgcgagctt
     1921 gagctcgtca ctgatccggg tcagatcagc gccgcgctcg gccagctccg cgcgcagccg
     1981 gtcattttca agccgcgagt ccctcagcga gaaatagttg tgccagagcc ccgagatggt
     2041 cccgacggtc ttggccgtcg cccactggaa cggcgcgagc gccgccatga gccagacctg
     2101 tgcgtaacgc tcgtcgcccc gggccgagag gccctggacg cggttgagcg agatcacgcc
     2161 catgtgagcg acgagcagga tcgcgagcag gaccgtcggg tttttcttgg tcttgatctt
     2221 aaagctcaca ggggtcagct gtcagcgctc agctttcagc ggtcagctcg tgccctttgg
     2281 cgggggatca gcagtctcgg cggggggcgg gctgagagct gagagctgac tgctacgaag
     2341 gtaatgtcgt cccgtcccag cgcacgcggc gcaggaggtc gaagtcagag agcatggtcc
     2401 cagcgccgag gacgaccgac gagagcgggt cctcggccat cgagaccggc agcccggtct
     2461 cctgcatgag ccggacgtcg agtttcttga gcatggcgcc gccaccggtg aggacgatcc
     2521 cgcggtcgac gatgtcggcc gagagctcgg gcggggtgcg ctcgagcgcg acccggacgg
     2581 cgttgatgat cgtcgccacc gagtcggcga gggcctcgcg gatctcctcg tccgagacga
     2641 cgatcgtttt cgggatgccc tcgatcaggt tgcgcccctt gatctccatg gtcatcggct
     2701 cgtcgacggg cgccgccgac ccgatctcga tcttgatcga ttcggccgtg cgctcgccga
     2761 tgagcaggtt gtacttgcgc ttgatgtact gcgtgatcgc ctcgtccatc tcgttgccgg
     2821 cgacgcgcac gctcttggag tagacgatgc ccgacatcga gatgacggcg atgtcggtcg
     2881 tcccgccacc gatgtcgacg atcatgttgc cgtgcggctc ggtgatcggc agccctgcgc
     2941 cgatggccgc agccatggcc tcctcgacga ggtaaacctc cgaggccttg gcgcggtagg
     3001 cgctgtcctc gaccgcccgg cgctcaacct gggtgatctc cgacggaatg ccgatgacga
     3061 tccgcgggct gacccactgc ttgccgttgt gcgccttctg gatgaagtac tggagcatct
     3121 tctcggtgac ttcgaagtcg gcaatgacgc cgtccttcat cgggcggata gcgacaatgt
     3181 tgcccggcgt ccgcccgagc atctccttcg cctccttacc cacggcctcg accttgtggg
     3241 agaccttgtt gatcgccacg atcgacggct cagagaccac gatcccgcgc ccgcgcgcga
     3301 agaccagagt gttggcggtg ccgaggtcga tggcgagatc
//
LOCUS       DASRIG010000477         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_747709, whole genome shotgun sequence.
ACCESSION   DASRIG010000477 DASRIG010000000
VERSION     DASRIG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747709"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VFB52_12810"
     CDS             <1..1016
                     /locus_tag="VFB52_12810"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936882.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZO07261.1"
                     /translation="TAYITENSTIVGAPGLEDEHLPVFDVAVGERAISHTGHIDMMAA
                     TQPFISGAISKTVNLPQTAGVEDIADAYTRGWKGGLKALAIYRDGSKTAQALRTDAQE
                     KKEEAVEMAQPVRKKMPRERESITHKFSIAGHEGYITAGKYDDGTVGEIFLTDIGKEG
                     STMRGLLNAYATAISLGLQYGVPLETFVRKFSYMRFDPEGMTGNPEIPFAKSLPDYIM
                     RWVASRFIEDTETLEELGIMTQEVRNKKEAETSQLSIGLSSDTAGPANGGNGHGNGNG
                     GAAKAGESQPAAAAFTETPPVVPAKMQGLDLGPACEQCGGMMQRTGSCYTCSSCGNNT
                     GCG"
     gene            1033..1617
                     /locus_tag="VFB52_12815"
     CDS             1033..1617
                     /locus_tag="VFB52_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07262.1"
                     /translation="MRSGRGELSKAVSIVAIGLLCGIAIVGIGAVVSGPEEDTAVGRV
                     VFAAAALTVFSLIGMAGVLLAVRRPSLNWFGYLTVVLALVAFGLVTWKLWDGFFFFGN
                     DWELPIAVTVAALACGQVSLLLAWARDNGPVAWVALGAGSMIVAIAALTVLTIAFDDV
                     DVDGRVYGVLAILYLLGIGLLPLLTLSGGDQEGQ"
     gene            1614..2147
                     /locus_tag="VFB52_12820"
     CDS             1614..2147
                     /locus_tag="VFB52_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07263.1"
                     /translation="MRNLAALLATAVLLTASSFALAAPRESNLATCDDAILGEGPDDW
                     RSRAITAGPVSVFKQPLARMTRTKNGLVAKMPMLIRGRAPVTVSVPPPLRGRVFLYYG
                     RVIGRDGKPTTSVNGARGYGETRFELCQGRKRTPFPGGIRVRGTEPVRLTIGVEGETT
                     THTLRLGKPKVYFTPNR"
     gene            complement(2131..3486)
                     /locus_tag="VFB52_12825"
     CDS             complement(2131..3486)
                     /locus_tag="VFB52_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO07264.1"
                     /translation="MEAAVKGAAPINAPERELIRGLPPGPRMPRALQTAIWSRQAQWM
                     LAQSRARFGDVFTIRIAHEGDWVMVADPALVKQVFTGDPKVFHAGEGNQILRPILGDN
                     SVLVLDEKAHIGQRRLLLPPFHGERMQGYERKMAEIAAREVDSWPTGSPYKLRPRMQA
                     ITLEIILETVFGVHDRERMDPLRAALREFLDLTTDPRFLLPVVLAGPERVRRIPGFAR
                     RIDRVDELIYREIAERRAAGGLEERDDILSMLVGAEHEDGSPMSDAEIRDELLTLLVA
                     GHETTATALSWAVERLVRHPDKLERLRTEVLAGEDAYLTATIQETLRLRPVLVAVIRK
                     LTEPVELGGYLLPAGARVTPSIYLVHRDPGIYPDPHSFVPERFLDNPPGTYTWIPFGG
                     GVRRCLGAAFAQFEMAVVLRELVRRHRIEPARAGSERPFRRAITETPRHDAEVVLSGS
                     A"
BASE COUNT          583 a         1255 c         1334 g          556 t
ORIGIN      
        1 tcaccgccta catcaccgag aacagcacga tcgtcggcgc accaggcctc gaggacgagc
       61 atctgccggt cttcgacgtc gcggtcggtg agcgggcgat cagccacacc ggccacatcg
      121 acatgatggc cgccacccag cccttcatct ccggcgcgat ctcgaagacc gtgaacctgc
      181 cgcagacggc gggcgtcgag gacatcgccg acgcctacac gcgcggctgg aagggcggcc
      241 tcaaggcgct cgcgatctac cgcgacggct cgaagaccgc gcaggcgctg cgcaccgacg
      301 cccaggagaa gaaggaggag gcggtcgaga tggcgcagcc ggtgcgcaag aagatgccgc
      361 gcgagcgcga atcgatcacc cacaagttct cgatcgccgg ccacgagggc tacatcaccg
      421 ccggcaagta cgacgacggc acggtcggcg agatcttcct caccgacatc ggcaaggagg
      481 gctcgacgat gcgcgggctg ctcaatgcct acgcgaccgc gatctcgctc ggcctccagt
      541 acggggtgcc gctggagacc ttcgtgcgca agttcagcta catgcgcttc gacccggagg
      601 ggatgaccgg caacccggag atcccgttcg ccaaatcgct gcccgactac atcatgcgct
      661 gggtcgcaag ccgcttcatc gaggacaccg agacgctcga ggaactgggg atcatgaccc
      721 aggaggtccg caacaagaag gaagcggaga cctcgcagct gagcatcggc ctgagcagcg
      781 acacggccgg cccggcgaac ggcggcaacg gtcacgggaa cggcaacggc ggcgcggcga
      841 aggccggcga gtcccagccc gcggcggccg ccttcaccga aacgcccccg gtggtcccgg
      901 cgaagatgca gggcctcgac ctcggcccgg cctgcgagca gtgcggcggg atgatgcagc
      961 ggaccggcag ctgctacacg tgttcttcct gcggtaacaa caccggctgc ggctgagcgc
     1021 taagttcccc ccgtgcgttc cgggcggggg gaactgtcga aggcggtctc gatcgtggcg
     1081 atcgggctgc tctgcgggat cgcgatcgtc gggatcggcg cggtcgtctc cggccccgag
     1141 gaggacacgg cggtcggacg ggtggtgttc gcggcagccg cgctgaccgt gttcagcctg
     1201 atcgggatgg ccggcgtgct gctcgccgtg cggcgtccgt cgctgaactg gttcggctac
     1261 ctgacggtgg tgctcgcgct cgtcgccttc ggcctggtga cctggaagct ctgggacgga
     1321 ttcttcttct tcggcaatga ctgggaactg ccgatcgcgg tcacggtcgc cgcgcttgcc
     1381 tgcggtcagg tctcgctgct gctcgcctgg gcgcgtgaca acggcccggt tgcctgggtt
     1441 gcgctcggcg ccggctcgat gatcgtcgcg atcgcggcgc tgacggttct gacgatcgcg
     1501 tttgacgacg tcgacgtcga cgggcgcgtc tacggcgtgc tcgcgatcct ctatctgctt
     1561 gggatcggcc tgttgccgct gctcactttg agcggcggcg accaggaagg gcaatgagga
     1621 acctggcggc gctgctagcg accgcggtcc tgctgaccgc gtcgtcgttc gccttggcag
     1681 ccccgcgcga atcgaacctc gccacctgcg acgacgcgat cctgggcgaa gggcccgacg
     1741 actggcgctc gcgggcgatc accgccgggc cggtcagcgt gttcaagcag ccgctcgccc
     1801 gcatgaccag gacgaaaaac ggtctggtcg cgaagatgcc gatgctgatc cggggccggg
     1861 cgccggtgac cgtgagcgtg ccgccgccgc tccgcggccg ggtcttcctc tactacggca
     1921 gggtgatcgg ccgcgacggg aaaccgacga ccagcgtgaa tggcgcccgc ggctacggcg
     1981 aaacccggtt cgagctttgc caggggcgca aacggacgcc gttcccgggc gggatccggg
     2041 tccggggcac ggaaccggtg cgcctgacga tcggggtcga aggtgagacg accacccaca
     2101 cgctgcgact cggcaagccg aaggtctact tcacgccgaa ccgctgagca cgacctcggc
     2161 gtcgtgccgg ggcgtctcgg tgatcgcgcg acggaagggg cgctcggagc cggcccgagc
     2221 gggctcgatc cggtggcggc ggaccagctc acgcaggacg acggccatct cgaactgggc
     2281 gaaggcggcg ccgaggcagc ggcgcacgcc gccgccgaag gggatccagg tgtaggtgcc
     2341 gggcgggttg tcgaggaagc gctcggggac gaaggagtgc gggtcgggat agatccccgg
     2401 atcgcggtgg accaggtaga tgctcggggt gacccgggcg ccggccggca gcaggtagcc
     2461 gcccagctcg accggctcgg tcagcttgcg gatcaccgcc acgagcaccg ggcgcaggcg
     2521 cagggtctcc tggatcgtcg cggtcaggta ggcgtcctcg ccggcgagca cctcggtgcg
     2581 caggcgctcg agcttgtccg ggtggcggac caggcgctcg accgcccagg aaagcgcggt
     2641 cgcggtcgtc tcatggccgg cgacgagcag ggtgagcagc tcgtcgcgga tctcggcgtc
     2701 gctcatcggg ctgccgtcct cgtgttcggc gccgaccagc atcgagagga tgtcgtcgcg
     2761 ctcctcgaga cctccggcgg cgcggcgctc ggcgatctcg cggtagatca gctcgtcgac
     2821 ccggtcgatg cgacgggcga agccggggat gcggcgaacc cgctcgggcc cggcgagcac
     2881 gaccggcagc aggaagcgcg ggtcggtggt gaggtcgagg aactcgcgca acgccgcccg
     2941 cagcgggtcc atgcgctcgc gatcgtgcac accgaagacg gtctcgagga tgatctcgag
     3001 cgtgatcgcc tgcatccgcg gccgcagctt gtaggggctg ccagtcggcc aggagtcgac
     3061 ctcacgggcg gcgatctcgg ccatcttgcg ctcgtagccc tgcatgcgct cgccgtggaa
     3121 gggcggcagc aacaggcggc gctggccgat gtgggccttc tcgtcgagga cgaggaccga
     3181 gttgtcgcca aggatcggcc gcaggatctg gttgccctcc cccgcgtgaa agaccttcgg
     3241 gtcgccggtg aagacctgct tgaccagggc cgggtcggcg accatcaccc agtccccctc
     3301 gtgcgcgatc cggatcgtga acacgtcgcc gaaccgcgcc cggctctgcg ccagcatcca
     3361 ctgcgcctgc cgcgaccaga tcgcggtttg cagcgcccgc ggcatccgcg gccccggcgg
     3421 caacccccgg atcagctcgc gctccggagc gttgatcggc gccgcccctt tgaccgcagc
     3481 ctccatcgcc tccgaggcta ctccgatagc tgcaccgaaa tgtgtgacgt tcgtccgggg
     3541 atcaaatcga tccggctctt gacgcctata ccgggaacaa gtgccggatc gatcgaacga
     3601 ctaccgcagg tcctcgcagg cgatcccgtc gtggtcggcg tccagccggt gtgggtcctt
     3661 cttcggtccg ccgtgcttct tgtagaagcg ctgggcctgc cgccaggtgt tgaagtccga
     3721 gcagtcct
//
LOCUS       DASRIG010000478         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3088869, whole genome shotgun sequence.
ACCESSION   DASRIG010000478 DASRIG010000000
VERSION     DASRIG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088869"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFB52_12830"
     CDS             <1..1037
                     /locus_tag="VFB52_12830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO07265.1"
                     /translation="RYEPDVIVSTYPGVTAVLGMLRENRRLPIPVQSAITDLAGLRYW
                     VHPGVDMHYVTHPESIEEVEALAGPGSVRWTRPPISSDFLMPRTRRDAREALDVPAHA
                     RMVLVSGGGWGIGDLEGAIESALADDDTLVVCITGRNEGMRDRLEARFGDNEKVRILG
                     FTEQMSDWMAAADAMIHATAGLTVLEAHIRGCPVVSYGFNAGHLRANNAAFERFGLAE
                     VARSEHELGSMLRHVTGERRSPDSSFASLPSIASQALTVRSRVRPQPVWRLRTERVAA
                     AAVLAIVALVLATALIQWESPLKVTKPVTSRIHIGKEAEADAKPAAQVTGQSDADSSA
                     ETEEAAASTP"
     gene            1034..1738
                     /locus_tag="VFB52_12835"
     CDS             1034..1738
                     /locus_tag="VFB52_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZO07266.1"
                     /translation="MIAAAAAGAGLGAAAVAYAGPALAPVVPPLGNALGVVLREEGAE
                     GVALTFDDGPHPEGTPAVLEVLREHDAVATFFLAGEQVVQRPSLVAEIVAAGHRVELH
                     CYRHRNQLRLGARSLLDDADRAKAAIEEAGGQEVLDYRPPLGIFSAVGLRAVRSRGWR
                     PVLWSLWGRDWDKRATPTSIARRASAGARAGDVLLLHDADYYSARGSWIRTVAALPMI
                     LEDLGERGLRSRLLRR"
     gene            complement(1945..>2679)
                     /locus_tag="VFB52_12840"
     CDS             complement(1945..>2679)
                     /locus_tag="VFB52_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07267.1"
                     /translation="DGRLWDGVVDRLADRFRCIAPDWPIGSQQIAMDPGADLSPPGVA
                     ATIADLLTKLDLTDVTIVGNDSGGAMSQVLVTRHPERISRLVLTNCDTHENFPPGIFK
                     HLPALAKMPGGMALIALPFRSQAICRRAFAPFTKAPIPPELIASWMRPAQTDPAIKRD
                     LKKVTVGMHRRYTLEAAEKLRGSQLPILLTWAPGDKLFPISYAERLAGDVANATLVPI
                     PDSSTFVPLDQPQRLSEEIARFAGTA"
BASE COUNT          391 a          911 c          969 g          408 t
ORIGIN      
        1 cccggtacga acccgacgtg atcgtctcca cctacccggg cgtcaccgcg gtgctgggga
       61 tgctgcgcga gaaccgccgc ctgccgatcc cggtgcagtc ggcgatcacc gacctcgccg
      121 ggctgcgcta ctgggtccat cccggcgtcg acatgcatta cgtcacccac ccggagtcga
      181 tcgaggaagt cgaggcgctc gccggcccgg gcagcgtgcg ctggacgcga ccgccgatct
      241 ccagcgactt cctgatgccg cgcacccgcc gcgacgcccg cgaggcgctc gacgttccgg
      301 cccacgcccg catggtcctg gtctctggcg gaggctgggg catcggcgac ctcgagggcg
      361 cgatcgagtc ggcgctggcc gacgacgaca cgctggtggt ttgcatcacc ggccgcaacg
      421 agggcatgcg cgaccgtctc gaagcgcgct tcggcgacaa cgagaaggtg cggatcctcg
      481 gcttcaccga gcagatgagc gactggatgg cggcggccga cgcgatgatc cacgcgaccg
      541 ccggcctgac cgtgctcgag gcccacatcc gcggctgccc ggtcgtctcc tacgggttca
      601 acgctggcca cctgcgcgcc aacaacgccg ccttcgaacg cttcggcctc gccgaagtgg
      661 cccgctccga gcacgagctg gggtcgatgt tgcgccacgt caccggcgaa cggcgctcgc
      721 ccgactcctc tttcgcctcg ctgccctcga tcgcctcgca ggcgctcacg gtgcgctcgc
      781 gggtgcgccc gcagccggtc tggcgcctgc gtaccgagcg ggtcgccgca gcggccgtgc
      841 ttgcgatcgt ggccctagtg ctggccaccg cgctgattca gtgggagagc ccgctgaagg
      901 tcacgaagcc ggtgaccagc cgcattcaca tcggcaagga ggctgaagcc gatgccaaac
      961 ccgcggctca ggtgacgggc cagtccgacg cggactcctc ggcggaaacc gaagaggccg
     1021 cggcgagcac gccttgatcg ccgcggcggc ggcgggagcc gggctcggcg ccgccgcggt
     1081 cgcctacgcc gggccggcgc tggcgccggt cgtcccgccg ctcggcaacg cgctcggcgt
     1141 cgtcctgcgc gaagagggcg ccgagggcgt cgcgctcacc ttcgacgacg gcccccaccc
     1201 cgagggcacg ccggccgtgc tcgaggtgct gcgcgagcat gacgccgtcg cgaccttctt
     1261 cctcgccggc gagcaggtgg tgcagcggcc ctcgctggta gccgagatcg tcgccgccgg
     1321 ccaccgggtc gagctgcact gctaccgcca ccgcaaccag ttgcggctcg gcgcccgctc
     1381 gctgctcgac gacgccgatc gcgccaaggc ggcgatcgag gaagcgggag ggcaggaggt
     1441 gctggactac cggccgccgc tggggatctt cagcgcagtc ggcctgcgcg cggtgcggag
     1501 ccgtggctgg cgccccgtgc tctggtcgct gtgggggcgg gattgggaca aaagagcgac
     1561 gccaacttcg atcgcgcggc gcgcgagtgc cggtgcccgt gccggagacg tgctgctgct
     1621 acacgatgcc gactactaca gcgcgcgcgg ctcctggatc cgcaccgtgg cggcgctgcc
     1681 gatgatcctc gaggacctcg gggagcgggg gctgagatcg aggctgctcc gacgctgact
     1741 cggcggggcg agcgtccaca cgtcgcaacc ctcgaacgct tcgtcgagtt gctgtgccat
     1801 ggtgctcttg ggccgatctt ggtcgaagat ctgcttggac atagctctcc gtcctgtgta
     1861 ggggggtcga taaagattcg cctcccagtc ggacgaaata tctgcacttt gcgaaaaagc
     1921 caggaatttg ggcgattcgc gcgttcaggc ggttcccgca aaccgcgcga tctcctcgct
     1981 gaggcgctgc ggttggtcga gcggaacgaa ggtgctggag tcgggaatcg gcaccaaggt
     2041 ggcgtttgcg acatcgccgg cgagccgttc cgcataggag atcgggaaca gcttgtcgcc
     2101 cggcgcccag gtcagcagga tcggcaactg cgagccgcgt agcttctccg ctgcctccag
     2161 cgtgtagcgg cggtgcatgc cgacggtcac cttcttgagg tcgcgtttga tcgccggatc
     2221 ggtttgcgcc ggccgcatcc aggaggcgat caactcgggt gggatcgggg ccttggtaaa
     2281 cggcgcgaag gctcgacggc agatcgcctg ggagcggaag gggagcgcga tcagggccat
     2341 gccgccgggc atcttcgcca gcgccggcag gtgtttgaag atcccgggcg ggaagttctc
     2401 gtgggtgtcg cagttggtca ggaccaggcg ggagatccgc tcggggtggc gggttaccag
     2461 cacctgcgac atcgcgccgc cggagtcgtt gccgacgatc gtcacgtcgg tcaggtcgag
     2521 cttggtgagc aagtcggcga tcgtcgcggc gacgccggga ggggagaggt cggcaccggg
     2581 gtccatcgcg atctgctggg agccgatcgg ccagtccggg gcgatacagc ggaagcggtc
     2641 ggcaaggcgg tcgaccaccc cgtcccacag ccggccgtc
//
LOCUS       DASRIG010000479         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_984257, whole genome shotgun sequence.
ACCESSION   DASRIG010000479 DASRIG010000000
VERSION     DASRIG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984257"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFB52_12845"
     CDS             complement(<1..209)
                     /locus_tag="VFB52_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07268.1"
                     /translation="MVDVRVSSYARYRPHKSTRTLRSKAAARELDGIPGMARVEGSGD
                     SRNVLYFFAAHKLGRLYPDLEKVKC"
     gene            245..994
                     /locus_tag="VFB52_12850"
     CDS             245..994
                     /locus_tag="VFB52_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZO07269.1"
                     /translation="MPAQAYAGKECVCQFRKGICDQTCVRDMLETPFYIACLKLSGRR
                     CLVVGGGDIGLEKVEGLLACDGDVVLIAPTAHPELESLAAEGSIAWEKREYAGPEDLE
                     GVFMVIAATDDSEVNIKVFDDAEQRAMLVNIVDVPPLCNFILPAIVRTGPLAIAISTA
                     GASPALAKRMKREISDLFGEEYARLAVMLNDARGWAKGTLPTYDDRKEFFEGIVNGDP
                     DPIELIRAGDESAVLAIIESAKERATAALAA"
     gene            complement(991..1491)
                     /locus_tag="VFB52_12855"
     CDS             complement(991..1491)
                     /locus_tag="VFB52_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO07270.1"
                     /translation="MSETASPTAPVQENANHKSCGGTPHREAWLSLARAHAAITGRMQ
                     EALTAAGMPPLPWFEVLATISEAPEQRLKMGELAEALVMTRGGLTKLVDRLIKAGLLE
                     RTFCETDRRVSYATLLPAGAELLEEMGPVVSAELDVAFSANLSEGEADVLRETLERLR
                     GSACEA"
     gene            1624..2175
                     /locus_tag="VFB52_12860"
     CDS             1624..2175
                     /locus_tag="VFB52_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZO07271.1"
                     /translation="MSTETLNQQIPAGTWTVDPVHSSIQFGVTHGGVSTFRSGFGEYA
                     AKLTGGEAPRLEGTVDVASIDIDEPQFKAHVMSPEFFDAEQHPQLKFSSTELSVADDG
                     SVRLSGELEIRGETREVNAVGRFAKVPAYLDGNERIGLSVEAAVDRREFGLNWQADLP
                     AGGIALEYEVALNVELQFVAEAE"
     gene            2178..2729
                     /locus_tag="VFB52_12865"
     CDS             2178..2729
                     /locus_tag="VFB52_12865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZO07272.1"
                     /translation="MRVLGISGSLRRDSYNSALLRVAAERLPAGAELVEYEGLAAIPP
                     YDADVEEEGTPAAVEELRELVRNADAVLLATPEYNHSLPGQLKNALDWISRPAGKSAL
                     MGKPAAAIGASTGMFGAVWAQAEARKVLGALGGRVVEAELPVPRAAAQYHDGELELAP
                     EQAQRLTEILSELLAEVEELAVA"
     gene            complement(2749..3114)
                     /locus_tag="VFB52_12870"
     CDS             complement(2749..3114)
                     /locus_tag="VFB52_12870"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HZO07273.1"
                     /translation="MPSSVSLRLSILLAALALLAVPAVAQASWGSIAIDPETGAKGIA
                     AGKNTAKAAKDAAKNRCGDPHCKSAVWVFNGWGATVHKKNGVYISGIGRTKAKAIENA
                     RKRANEKAPLVASVFSGLS"
     gene            complement(3147..3881)
                     /locus_tag="VFB52_12875"
     CDS             complement(3147..3881)
                     /locus_tag="VFB52_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZO07274.1"
                     /translation="MVDELRKSAERVGERASAAKDAVGEKASDAIAAVKPKLRGVSHK
                     WAFFASLVLGVALILLAKTPKATLAVAIYAISLSALFGTSALYHGVNWKRPDVRMWMR
                     RLDHSMIFFLIAGTYTPFALLVLNGALATAILVVVWTGAIAGAIVETVWIDHPKWVSA
                     LIYLALGWVAVVAFPDLWSSLGVAGAALVVAGGVLYTAGAVVYATQRPNPSPAIFGYH
                     EVFHLFVIAAAVAHFSAVAFFALPQA"
     gene            4144..>5180
                     /locus_tag="VFB52_12880"
     CDS             4144..>5180
                     /locus_tag="VFB52_12880"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578774.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZO07275.1"
                     /translation="MHPFDQIEWEIRDAVIGNPESPAFEQRGVEFPKSWSQNATNIVA
                     QKYFRGKLGSPERESSVKQMIGRVAGTIANWGRELGYFASKEDGDAFEDELTAILLHQ
                     KAAFNSPVWFNVGFEEQPQCSACFILSVDDTIESILEWNTKEGMIFRGGSGSGINLSN
                     IRGSVEHLSKGGLASGPVSFMRGADAWAGTIKSGGKTRRAAKMVVLDVDHPDIRDFIR
                     CKANEEEKAAALRDAGFDMRIDGDGFTSIQYQNANNSVRVTDDFMRAVEAGEDWDLLA
                     RMTGEALETVPSRELMREIADAAWRCADPGVQYDTTINRWHTCPQSGRINASNPCSEY
                     MHVDDSACNLAS"
BASE COUNT          895 a         1729 c         1754 g          802 t
ORIGIN      
        1 ctgcacttaa ctttttcgag gtcgggatag agccgtccga gcttgtgggc ggcgaaaaag
       61 tacaagacgt tccgggagtc acccgacccc tccacacggg ccatcccggg gattccgtct
      121 agctcccggg ccgcagcctt agatcgaagc gtccgtgtcg acttatgcgg cctatagcgc
      181 gcataactcg acacgcgcac gtcaaccacg gccggaaagt ccgggatcac taacctttgg
      241 ctcgatgcca gcccaggcct atgccggcaa ggagtgcgtc tgtcagttcc gcaaagggat
      301 ctgcgatcag acctgcgtgc gggacatgct ggagacgccg ttctacatcg cctgcctgaa
      361 actgagcggg cggcgctgcc tggtcgtcgg cggcggtgac atcgggctgg agaaggtcga
      421 ggggctgctc gcctgcgacg gcgacgtcgt cttgatcgcg ccgaccgcgc acccggagct
      481 ggagtcgctg gcggcggagg gctcgatcgc ctgggagaag cgcgagtacg ccggtccgga
      541 ggatcttgaa ggcgtgttca tggtgatcgc cgcgaccgac gacagcgagg tcaacatcaa
      601 ggtcttcgac gacgccgagc agcgggcgat gctggtcaac atcgtcgacg tgccgccgct
      661 gtgcaacttc atcctgccgg cgatcgtgcg caccgggccg ctggcgatcg cgatctcaac
      721 cgcgggcgcc tcgcctgcgc tggccaagcg gatgaagcgc gagatctccg acctcttcgg
      781 cgaggagtac gcgcgcctgg cggtgatgct gaacgacgcc cgcggctggg ccaagggcac
      841 gctgccgacc tacgacgacc gcaaagagtt cttcgagggc atcgtcaacg gcgatcccga
      901 cccgatcgag ctgatccggg ccggcgacga gagcgccgtg ctggcgatca tcgagtccgc
      961 caaagagcgg gccaccgcag cgctcgccgc ttaggcttcg caggcgctgc cgcgcaaacg
     1021 ctcgagcgtc tcgcgcagca cgtccgcctc accctcgctg aggttggcgg agaaggcgac
     1081 gtcgagctcg gcgctgacca ccggccccat ctcctcgagc agctcggccc cggccgggag
     1141 cagggtcgcg taactgaccc ggcgatcggt ctcgcagaag gtccgctcca gcaggcccgc
     1201 cttgatcagg cggtcgacga gcttggtgag gccaccgcgg gtcatcacga gggcctcggc
     1261 cagctccccc attttgagcc gctgctcggg cgcctcggag atcgtcgcca ggacctcgaa
     1321 ccagggcaac ggcggcatcc cggctgccgt cagcgcctcc tgcatccggc cggtaatcgc
     1381 cgcgtgagcg cgtgccagcg agagccaggc ctcgcgatgg ggcgtgccgc cgcagctctt
     1441 gtggttggcg ttctcctgga caggagcggt cggggaggcg gtctcagaca tatgtgcatg
     1501 atcgcataag aaggttgatc tgacaatagt tgaggacaca agtatttctg ctacgattgt
     1561 ttctgcaaca accatttgcg cagcaacaac ttcgaacacc cccccccacg aaaggcagca
     1621 gaaatgagca ccgagaccct caaccagcag attccggcgg gcacctggac cgtcgacccc
     1681 gtccactcct cgatccagtt cggcgtcacc cacggcggcg tctccacctt ccgcagcggc
     1741 tttggcgagt acgccgcgaa gctcacgggc ggcgaggcgc cgcggctcga gggcacggtc
     1801 gacgtcgcca gcatcgacat cgacgagccg cagttcaagg cccacgtgat gagcccggag
     1861 ttcttcgacg ccgagcagca cccgcagctc aagttcagct cgaccgagct gagcgtcgcc
     1921 gacgacggca gcgttcgact cagcggcgag ctcgagatcc gcggcgagac ccgcgaggtc
     1981 aacgcggtcg gccgctttgc caaggtcccg gcctacctcg acggcaacga gcggatcggc
     2041 ctctcggtcg aggccgcggt cgaccggcgt gagttcggcc tcaactggca ggccgacctg
     2101 cccgccggcg gcatcgccct cgagtacgag gtcgccctca acgtcgagct ccagttcgtc
     2161 gcggaggccg agtaaccatg cgggtcctgg gcatatccgg aagcctgcgc cgggactcgt
     2221 acaacagcgc cctgctgcgg gtggccgccg agcggctgcc cgccggggcg gagttggtcg
     2281 agtacgaggg cctggccgcg atcccgccct acgacgccga cgtcgaagag gagggcacgc
     2341 cggcggcggt cgaggagctg cgcgagctgg tgcggaacgc cgacgcggtg ttgctcgcca
     2401 cgcccgagta caaccactcg cttccgggac agctgaagaa cgccctcgat tggatttcgc
     2461 ggccggccgg caagagcgcc ctgatgggca agccggcggc ggcgatcggc gcctcgaccg
     2521 gcatgttcgg cgcggtctgg gcgcaggccg aggcccgcaa agtcctcggc gccctgggcg
     2581 gtcgcgtcgt cgaggccgag ctgcccgtcc cgcgcgcggc cgcccagtac cacgacggcg
     2641 agctggagct ggcgcccgag caggcccagc gcctcaccga gatcctcagt gagctgctcg
     2701 ccgaagtgga ggagctggcg gtcgcctgac ggcgaccgcc acgcccgctc agctcaggcc
     2761 gctgaacacg ctcgcgacga gcggggcttt ttcgtttgcc cgcttgcgcg cgttttcgat
     2821 cgccttcgcc ttggtgcggc cgatcccgga gatgtagacg ccgttcttct tgtgcaccgt
     2881 cgcgccccag ccgttgaaga cccagacggc ggacttgcag tgcgggtcgc cgcagcggtt
     2941 cttggctgcg tccttggcgg ctttggcggt gttcttgccg gccgcgatcc ccttcgctcc
     3001 ggtttccggg tcgatcgcga tcgagcccca ggaagcctgg gcaacggcgg gaacggcgag
     3061 aagcgcgagc gcggcgagca ggattgagag tcgcagggat acggacgagg gcataggggg
     3121 gcctttcgtt cgggcccagg cggcgtttat gcctggggca gggcgaagaa cgctaccgcg
     3181 gagaagtgcg ccacggcggc ggcgatgacg aatagatgaa agacctcgtg gtacccgaag
     3241 atcgccgggc tcgggttcgg ccgctgggtc gcgtagacga ccgcgcccgc cgtgtagagg
     3301 actccaccgg cgacgaccag cgccgccccg gcgacgccga gcgaggacca gagatccggg
     3361 aaagcaacga ccgcgaccca gccgagcgcc aggtagatca gcgccgagac ccatttcggg
     3421 tggtcgatcc agaccgtctc gacgatcgcg ccggcgatcg cgccggtcca gacgacgacc
     3481 aggatcgcgg tcgccagggc gccgttgagg acgagcagcg cgaacggcgt gtaggtgccg
     3541 gcgatgagga agaagatcat cgagtgatcg agccggcgca tccacatgcg cacgtccggc
     3601 cgcttccagt tgaccccgtg gtagagggcg ctggtgccga agagggcgga gaggctgatc
     3661 gcgtagatcg ccaccgccag ggtcgccttc ggcgtcttcg cgagcaggat cagcgccacc
     3721 ccgagcacca gcgaggcgaa gaacgcccac ttgtgggaga cgccacgcag tttcggtttg
     3781 accgcggcga tcgcatcgga ggccttctcc ccgaccgcgt ccttcgccgc cgaggcccgt
     3841 tccccgaccc gctcggcgct tttgcgcaac tcgtcgacca tctagcctca gagtagcggg
     3901 agatcggttt gccgagttag cggagttgtc agaatgtaag aacgctttcg cgcgcctgtg
     3961 gcgttaattt gcgcgcgttc cgtgtacgtg agacgtgttt ggcgtccgag ccgggagcaa
     4021 tcctcctctg ttacccctaa aagtcgcttt gagaggagaa gtttgatgtc caagaccgcc
     4081 actaccagtc ccaaaaacgg tctgcagggc ctctctgttg agcggcgctt ttcggctgcc
     4141 ggcgtgcatc ctttcgacca gatcgagtgg gagatccgcg acgccgtgat cgggaatccg
     4201 gaaagtccgg ccttcgagca gcgcggggtc gagttcccca agagctggtc ccagaacgcg
     4261 accaacatcg tcgcccagaa gtacttccgc gggaagctcg gcagccccga gcgcgagagc
     4321 tcggtcaaac agatgatcgg ccgggttgcc ggcacgatcg ccaactgggg gcgtgagctg
     4381 ggttacttcg ccagcaaaga ggacggcgac gccttcgagg acgagctgac cgcgatcctg
     4441 ctgcaccaga aggcggcctt caactcgccg gtctggttca acgtcggctt cgaggagcag
     4501 ccgcagtgct cggcctgctt catcttgagc gtcgacgaca ccatcgagtc gatcctcgag
     4561 tggaacacca aagagggaat gatcttccgc ggcggctcgg ggtcggggat caacctctcc
     4621 aacatccgcg gctcggtcga gcacctctcc aagggcggcc tggcctccgg cccggtcagc
     4681 ttcatgcgcg gggccgacgc ctgggcggga acgatcaagt ccggcggcaa gacccgccgc
     4741 gccgcgaaga tggtcgtgct cgacgtcgac catcccgaca tccgcgactt catccggtgc
     4801 aaggccaacg aggaggagaa ggccgccgcg ctccgcgacg ccggattcga catgcggatc
     4861 gacggcgacg gcttcacgtc gatccagtac cagaacgcga acaactcggt cagggtcacc
     4921 gacgacttca tgcgggccgt cgaggcggga gaggactggg accttttggc ccgcatgacg
     4981 ggtgaggccc tcgagaccgt cccgtcccgc gaactcatgc gcgagatcgc cgatgcggcc
     5041 tggcgctgcg cggaccccgg cgtccagtac gacaccacga tcaaccgctg gcacacctgc
     5101 ccccagtccg gccggatcaa cgcgtcgaac ccgtgctccg agtacatgca cgtcgacgac
     5161 tcggcgtgca acctcgcgtc
//
LOCUS       DASRIG010000480         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3324181, whole genome shotgun sequence.
ACCESSION   DASRIG010000480 DASRIG010000000
VERSION     DASRIG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324181"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VFB52_12885"
     CDS             complement(<1..1348)
                     /locus_tag="VFB52_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO07276.1"
                     /translation="MNPKVAAAAFERYRQPAHLREEWLRLGLTSDAVVGPTLYARLLQ
                     FPDNPAVVDGEVRLSNGDLATRSARLAGALQAAGVEAGDVVSWQVPNWWEALATALAV
                     WRIGAVNNPVLMIYREHELRQIFTDLEPKVVVAPQEFRRFRHQEMLDSVLAEIGHEPA
                     LRIGVRGGDGGWSDLEELVEASEAPPVETATGPDDPCVVAYTSGTTAGAKGVVIDSTS
                     LMAETRQMRETWGLAWDDVSFMPAPLAHLTGVTVGLTVPFTAGCSVVLMDSWRPDEAV
                     PTMEREGCTISSGTPIFLEEIVRGYEELGIERASLRQYSVGGQAVVPTLIERAEAVGI
                     GAFRCYGMTEHLSTTIANASSSMETRRDTDGPVGPGSEISCVDEEGRVLPPGEPGEIR
                     VRGPERMMGYVNPAHNEGVLDPEEGWFSTGDVGFVDEDGNVHISGRIKDIINRGGEK"
     gene            1556..2695
                     /locus_tag="VFB52_12890"
     CDS             1556..2695
                     /locus_tag="VFB52_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07277.1"
                     /translation="MDLSLPPAAEAVRREALGWLEDWLAANPSEAADPRLDLDLRRRY
                     QRDAYDEGWLVPSWPRGEGGREVDAETELWIKLDFARRGAPKLPNVQGPGVVAESLKG
                     FGSSEQREHVRAVARGDVWWCLGMSEPEAGSDLASLRTRAKQHDGGFTIDGQKVWTSH
                     ARESELCLLFARTGERDSGHRGISAFVVPMDAPGITVRKIDKIGVEDEEFCEVFFDGV
                     EVPESALLGPLNGGWKVAMTSLSHERDMVWIMNLVEIEEALRTIRAEFGRSPRPELEL
                     ELAELEADADSIWLSGLRGLASRLAGKPDHETTLLKLFSTEAAQRAFLLAARACGPEA
                     AILAGSAHGAGVAAGEIEALGATIYGGTSEVQRNIVGERILGLPR"
     gene            2692..>3197
                     /locus_tag="VFB52_12895"
     CDS             2692..>3197
                     /locus_tag="VFB52_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07278.1"
                     /translation="MTAQPMTFADDEAVRELRRRLRRMIEEDLPEGFRGAFVEGTVGQ
                     ETANDFCRRLAEENLLTMSWPREFGGSDASIWEQAALREEMWAHHEPRGAQYMGLNWV
                     GPAIMQFGTAAQREKYLPAIANASSTWCQGFSEPEAGSDLASLRLRAQRTDGGGWSLS
                     GQKIWTSYA"
BASE COUNT          481 a         1126 c         1130 g          460 t
ORIGIN      
        1 acttctcgcc cccgcggttg atgatgtcct tgatccggcc ggagatgtgg acgttgccgt
       61 cctcgtcgac gaagccgacg tcgccggtcg agaaccagcc ctcctcggga tcgagcacgc
      121 cctcgttgtg ggcggggttg acgtagccca tcatccgctc gggcccgcgc acgcggattt
      181 cgcccggctc gcccggcggc agcacccggc cctcctcgtc gacgcagctg atctccgagc
      241 cggggccgac cggcccgtcg gtgtcgcgcc gcgtctccat cgagctgctg gcgttggcga
      301 tcgtcgtgct caggtgctcg gtcatcccgt aacagcggaa ggcgccgatc ccgaccgcct
      361 cggcgcgttc gatcagggtc ggcacgactg cctggccgcc gaccgagtac tggcgcagcg
      421 aggcccgctc gattccgagc tcctcgtaac cgcggacgat ctcctcgagg aagatcgggg
      481 tgccggaaga gatcgtgcag ccctcgcgct ccatcgtcgg caccgcctcg tcggggcgcc
      541 aggagtccat cagcacgacc gagcagccgg cggtgaaggg cacggtgagc ccgacggtga
      601 cgccggtcag gtgggcgagc ggggcgggca tgaaggagac gtcgtcccag gccaagcccc
      661 aggtctcgcg catctgccgg gtctcggcca tcagcgaggt cgagtcgatc accacgccct
      721 tggccccggc ggtggtgccc gaggtgtagg cgacgacgca ggggtcgtcg ggcccggtcg
      781 cggtctcgac cggcggcgcc tcggaagctt cgaccagctc ctccaggtcc gaccaaccgc
      841 cgtcgccacc gcgcacgccg atccgcagcg ccggctcgtg gccgatctcc gccagcaccg
      901 agtcgagcat ctcctggtgg cggaagcggc ggaactcctg cggcgcgacc acgactttcg
      961 gctcgaggtc ggtgaagatc tggcgcagct cgtgctcgcg gtagatcatc agcaccgggt
     1021 tgttgaccgc gccgatccgc cagacggcga gcgcggtggc cagcgcctcc caccagttgg
     1081 ggacctgcca ggagacgacg tcgccggcct cgaccccggc cgcctgcaac gccccggcga
     1141 ggcgcgccga gcgggtggcc aggtcaccat tacttagacg gacctcgccg tcgacgaccg
     1201 cggggttgtc cgggaactgc agcagccgcg cgtagagcgt cggcccgacg accgcatcgc
     1261 tggtcagacc gagccgcagc cactcctcgc gcaggtgcgc gggctggcgg tagcgctcga
     1321 acgcggcagc ggcgactttc gggttcatcg ggctctaacc acggctttct cctctccctg
     1381 ggacacctgg acggttatcc cacaaatcat caagatgata tagagtatga gacacaggcg
     1441 caattgcttg ccgaccgatc tcggaaggag accgagtatc gggggttgcg cgtaaccagg
     1501 cgaggcctga gaggagaatt tgcatcttga agtcgtccct gttcgtcccc gctcgatgga
     1561 cctgagcctg ccacccgcgg ctgaggccgt gcggagagaa gcgctgggat ggctcgagga
     1621 ctggctcgcg gccaacccct cggaggcggc cgacccgcgc ctggacctcg acctgcgccg
     1681 ccgctaccag cgcgacgcct acgacgaggg ctggctcgtg ccctcctggc cgcgcggcga
     1741 gggcggccgc gaggtcgacg ccgagaccga gctctggatc aagctcgact tcgcccgccg
     1801 cggcgccccg aagctgccca acgtccaggg ccccggtgtg gtcgccgagt cactcaaagg
     1861 gttcggcagc tccgagcagc gcgagcacgt gcgggcggtc gcccgcggcg acgtctggtg
     1921 gtgcctgggg atgtccgagc ccgaggccgg ctccgacctc gcctcgttgc gcacccgcgc
     1981 caagcagcac gacggtggct tcacgatcga cggccagaag gtctggacct cgcacgcgcg
     2041 cgagtccgag ctctgcctgc tgttcgcccg caccggcgag cgcgactccg gccaccgcgg
     2101 catctcagcc ttcgtagttc cgatggacgc cccggggatc acggtccgca agatcgacaa
     2161 gatcggggtc gaggacgagg agttctgcga ggtcttcttc gacggcgtcg aggtccctga
     2221 gtcagccctg ctcggtccgc tgaacggcgg ctggaaggtg gcgatgacct cactctcgca
     2281 cgagcgggac atggtctgga tcatgaacct ggtcgagatc gaggaggcgc tgcggacgat
     2341 ccgggccgag ttcggccgca gcccgcgacc ggagctggag ctggagctcg ccgagctcga
     2401 agccgacgcc gactcgatct ggctctccgg actgcgcggc ctcgccagcc ggctcgccgg
     2461 caagcccgac cacgagacca cactgctgaa gctgttctcg accgaggccg cccagcgggc
     2521 cttcctgctg gcggcccgcg cctgcggccc ggaggcggcg atcctcgccg gctcggccca
     2581 cggcgccggc gtcgccgccg gcgagatcga ggcgctcggg gcgacgatct acggcggcac
     2641 ctcggaggtg cagcgcaaca tcgtcggcga gcggatcctg gggctgccgc ggtgaccgcc
     2701 cagccgatga ccttcgccga cgacgaggcg gtgcgcgagc tgcgccggcg cctgcggcgg
     2761 atgatcgagg aggacctgcc ggagggcttc cgcggcgcct tcgtcgaggg cacggtcggc
     2821 caggagacgg ccaatgactt ctgccggcgc ctcgccgaag agaacctgct gacgatgagc
     2881 tggccgcgcg agttcggcgg ctccgacgcc agtatctggg agcaggcggc cctgcgcgag
     2941 gagatgtggg cccaccacga gccgcgagga gcgcagtaca tgggcctcaa ctgggtcggc
     3001 ccggcaatca tgcagttcgg gacggcggcc cagcgcgaga agtacctgcc ggcgatcgcc
     3061 aacgccagct cgacctggtg ccagggcttc agcgagccgg aggccggatc ggacctcgcc
     3121 tcactgcggc ttcgcgccca gcgcaccgac ggcggagggt ggtcgctcag cggtcagaag
     3181 atctggacct cctatgc
//
LOCUS       DASRIG010000481         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2547101, whole genome shotgun sequence.
ACCESSION   DASRIG010000481 DASRIG010000000
VERSION     DASRIG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547101"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..433)
                     /locus_tag="VFB52_12900"
     CDS             complement(104..433)
                     /locus_tag="VFB52_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07279.1"
                     /translation="MGQFDGQPSIRWSLRGLTEEGDEAWLIRGIARKLYHCPGCHGDI
                     DVGDEHTVVQFVRRLGGTDHHHWHRRCAEEILIPELGNLKRVPAGESTQTKLERRGRV
                     PSGRRRR"
     gene            complement(435..821)
                     /locus_tag="VFB52_12905"
     CDS             complement(435..821)
                     /locus_tag="VFB52_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07280.1"
                     /translation="MTLEDALRLTNRNMIAFDLLLGSAAIAAPAATLKVLGHDEPSPD
                     AKHLFRRCGPIWLTFAAAHAVAERRGSAADWQSLAWLRGTEIATDALWARSPAVTRPG
                     AKAGLVLAGVANVAMAAGFAWMSKRR"
     gene            872..1720
                     /locus_tag="VFB52_12910"
     CDS             872..1720
                     /locus_tag="VFB52_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZO07281.1"
                     /translation="MTKTATKSPQLKSLSDYIQERFDEFSRSQKDVARYIVDHLDEAA
                     FLTAEELARRASTSSSTVVRFSQALGFEGYPELQQAAIEEYRTTIAAAGSNSGALFSF
                     DHSELEGSLSADYSNLEETARKLTPEQVDASVSALATAQRIVIVGVDQMAFFASYLRH
                     TLSLLDIRAEIVASTGGESLQRLGRIDDETLVITLSAGRAHPLLLRAMKLALHRGART
                     LAISDATMSEVGEHAELTLYFSSNSPSFTRSNTALMALVQALAHGLYARDEAAHKDRI
                     RAYKLK"
     gene            1765..2652
                     /gene="pdxS"
                     /locus_tag="VFB52_12915"
     CDS             1765..2652
                     /gene="pdxS"
                     /locus_tag="VFB52_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZO07282.1"
                     /translation="MAERATFRLKTGLAEMLKGGVIMDVVTPEEAKIAEEAGACAVMA
                     LERVPADIRRDGGVARMSDPAMIKGIQEAVSIPVMAKARIGHFAEAQVLQALEVDYID
                     ESEVLTPADEVNHIDKLAFEVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGNVVEA
                     VRHMREINSGIKRLAQLRPEELAAAAKELPAPLEVVQEVAAAGRLPVPLFCAGGIATP
                     ADAALMMQLGAEGNFVGSGIFKSADPQRRAKAIVEATTHFNDPERVAAASIGLGEAMS
                     GLEIAPLAAADALLQNRGG"
     gene            2654..3238
                     /gene="pdxT"
                     /locus_tag="VFB52_12920"
     CDS             2654..3238
                     /gene="pdxT"
                     /locus_tag="VFB52_12920"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZO07283.1"
                     /translation="MRVGVLALQGDFEAHARTLRQLGAEASEVRTPEQLVGLDGLVIP
                     GGESTTIGKGIEAGGMEPAIRALHEAGTPILGTCAGLIVCDDTHLGLIDATARRNAFG
                     RQLQSFEADIEVEGIGDEPLRAVFIRAPWVERCGPGVEVLASYEGHPVAIREGSVLAC
                     AFHPELTDDSRFHAIFMAMATSASARDGAKEGTT"
     gene            3235..4338
                     /locus_tag="VFB52_12925"
     CDS             3235..4338
                     /locus_tag="VFB52_12925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZO07284.1"
                     /translation="MSDSRTQGLAKILVGYSTEVKAGEVVAIDGETAAAPLLLAVYEE
                     VLKAGGNPMLNVALDGQIAAYFKHASDKQLEWISPFASWMVDNADVRIAIGASTNTRE
                     LSGVPPEKQMLRQSVTGDLMQRAMKRSADGDFRWVYTLFPTSAYASEAEMSLADYEDF
                     YYGACLADAADPLSAWREASKETERLAEWIDGHEEVRVTAPGTDIKLGIAGRKFIPCD
                     GKHNMPDGEFFTGPIEDSVEGEVSFHLPAVIGGREVAGVKLRFEAGKVVDATAERGEE
                     FLLKMLDTDDGARTLGELGIGTNYGIDRGTREVLLDEKIGGTVHMAVGASYPESGGTN
                     ESAVHTDLVCDLRLGGKLEVDGVVMQEDGKFVV"
     gene            complement(4347..4586)
                     /locus_tag="VFB52_12930"
     CDS             complement(4347..4586)
                     /locus_tag="VFB52_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07285.1"
                     /translation="MAVMARDSWTDARLDDLKDRVDRGFAENRAEFRDVREEIREVHR
                     QIGALNRTIHQFMFAMVGTVFLGFMGTIAALVTLT"
     gene            complement(4688..>5047)
                     /locus_tag="VFB52_12935"
     CDS             complement(4688..>5047)
                     /locus_tag="VFB52_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07286.1"
                     /translation="AALSLWTAIPLTWVYIGSKVSHTQFPSGGPYVVVIVGILVSVVV
                     VAWLIGRLNDLYVRITGTNRLEPIRPTWLRSMRDATPVRGSVTVVEAVLMGSVMLAAL
                     ALTFWFFFLSGSPIPNQ"
BASE COUNT          869 a         1734 c         1714 g          730 t
ORIGIN      
        1 ggaagacgat cgccgcgagc agcagcaggg tgggcgccca ggaccagtag acgcggccga
       61 tgcgtgcgta gatggagacg agacgccgca tgccggggta acgctagcgg cgccggcgcc
      121 cggagggaac gcgtccgcgg cgctcgagct tcgtctgcgt cgactcccca gccgggacgc
      181 gcttcaggtt gccgagctcc gggatcagga tttcttcggc gcaacgccgg tgccaatggt
      241 gatgatcggt gccgccgagc cggcgcacga actggaccac ggtgtgctcg tcgccgacgt
      301 cgatgtcgcc gtgacagccg gggcagtggt agagcttgcg ggcgatcccg cggatcagcc
      361 aggcctcgtc gccctcttcg gtcaggccgc ggagactcca gcggatgctc ggctgaccgt
      421 cgaactggcc catgtcagcg cctcttcgac atccaggcga agccggccgc catcgccacg
      481 ttggcgacgc cggcgaggac gagcccggcc ttcgcgccgg gccgggtgac cgccggcgag
      541 cgcgcccaga gcgcgtcggt cgcgatctcg gtcccgcgca gccaggccag cgactgccag
      601 tcggcggcgc tgccacggcg ctcggcgacc gcgtgggcgg cggcgaaggt gagccagatc
      661 ggcccgcagc gccggaacag gtgcttcgcg tccggcgagg gctcgtcgtg acccagcacc
      721 ttcaaagtgg ctgccggggc ggcaattgcc gcgctcccga gcagcaagtc gaaggcgatc
      781 atgttgcggt tagtgaggcg cagcgcgtct tctagcgtca ttccaggagt ttctcagaag
      841 ggaaagcgtc cggttcgacg aattggacgc cgtgaccaag accgcaacga agtccccaca
      901 actcaaaagc ctttccgact acatccagga gcgtttcgac gagttctcgc gctcccagaa
      961 agatgtggct cgctacatcg tcgatcacct cgacgaggca gccttcctca ccgccgagga
     1021 gctggccagg agagcgagca cctcgtcatc gacggtggtg cgcttctccc aggccctcgg
     1081 cttcgagggc tacccggagc tccagcaggc cgcgatcgag gagtaccgca cgacgatcgc
     1141 cgccgccggc agcaacagcg gtgcgctttt ctccttcgac cactccgagc tcgagggatc
     1201 gctcagcgcc gactactcga acctcgagga aaccgcgcgc aaactgacgc ccgagcaggt
     1261 tgacgcttcc gtctcggcct tggccaccgc gcagcggatc gtgatcgtcg gcgtcgacca
     1321 gatggcgttc ttcgccagct acctccggca cacgctcagc ttgctcgaca tccgcgccga
     1381 aatcgtcgcc agcacgggcg gggagtcgct gcagcgactg ggtcggatcg acgacgaaac
     1441 gctggtcatc acgctctctg ccggacgcgc tcaccccttg ctgctgcggg cgatgaagct
     1501 cgctctgcac cgcggcgcgc gcaccctcgc gatctccgac gcgaccatgt ccgaggtcgg
     1561 cgagcacgcc gagctgacgc tctacttctc ctccaacagc ccctcgttca cgcgctcgaa
     1621 caccgccttg atggcgctcg tacaagcgtt ggcgcacggg ttgtacgcgc gggatgaagc
     1681 tgcgcacaaa gaccgcatcc gcgcctacaa actgaagtag gcccgccgac gagccggccc
     1741 cgcgcccccg taatatgggg cgcgatggcc gagcgcgcaa ccttccgact gaaaaccggc
     1801 ctcgccgaga tgttgaaagg cggggtgatc atggacgtgg tcacgcccga ggaggcgaag
     1861 atcgccgagg aggctggcgc ctgcgcggtg atggctctgg agcgcgtccc cgccgacatc
     1921 cgccgcgacg gcggcgtcgc ccgcatgtcc gacccggcga tgatcaaagg gatccaggag
     1981 gcggtgagca tcccggtgat ggcgaaggcc cggatcggcc acttcgccga ggcccaggtc
     2041 ctgcaggcgc tcgaggtcga ctacatcgac gagtccgagg tgctgacccc ggccgacgag
     2101 gtcaaccaca tcgacaagct cgccttcgag gttccgttcg tctgcggcgc caccaacctc
     2161 ggtgaggcgc tgcggcggat cggcgagggc gcggcgatga tccgctccaa gggcgaggcc
     2221 gggaccggca acgtggtcga ggcggtccgc cacatgcgcg agatcaactc cgggatcaag
     2281 cggctcgccc agctgcgccc cgaggagctt gccgccgctg ccaaagaact gccggcgccg
     2341 ctggaagtcg tccaggaggt cgctgccgcc ggacgcctgc cggttccgct cttctgcgcc
     2401 ggcggcatcg ccaccccggc ggacgcggcg ctgatgatgc agctcggcgc cgagggcaac
     2461 ttcgtcggct ccgggatctt caagagcgcc gacccgcagc ggcgcgccaa ggcgatcgtc
     2521 gaggcgacca cccacttcaa cgaccccgag cgggtcgccg ccgcctcgat cggcctcggt
     2581 gaggcgatgt cggggctgga gatcgccccg ctcgcggccg cagacgccct cctgcagaac
     2641 cgcggaggct gacctgcgcg tcggcgtctt agcgctgcag ggcgacttcg aggcgcacgc
     2701 ccgcacgctg cgccagctcg gcgccgaggc aagtgaggtg cgcacgcccg agcagctcgt
     2761 cgggctcgat gggctggtga tccccggggg ggagagcacc acgatcggca agggcatcga
     2821 ggcgggcggg atggagccgg cgattcgggc tctgcacgag gccggaacgc cgatcctcgg
     2881 cacctgcgcc ggcctgatcg tctgcgacga cacccacctc gggctgatcg acgcgaccgc
     2941 gcgccgcaac gccttcggcc gccagctgca gagcttcgag gccgacatcg aggtcgaggg
     3001 gatcggcgac gagccgctgc gcgccgtctt catccgcgcg ccctgggtcg agcgctgcgg
     3061 gcccggcgtc gaggtcctgg ccagctacga gggacacccg gtcgcgatcc gggaagggtc
     3121 ggtgctcgcc tgcgccttcc acccggagct gaccgacgac tcccgctttc acgcgatctt
     3181 catggccatg gccaccagcg cgagcgcccg cgacggcgcc aaggagggaa cgacgtgagc
     3241 gattcgagaa cgcagggact ggcgaagatc ctggtcggct actcgaccga ggtcaaagcg
     3301 ggcgaggttg tcgcgatcga cggcgagacc gcggcggcgc cgctgctgct tgccgtctac
     3361 gaggaggtgt tgaaggcggg cggcaacccg atgctcaacg tcgccctcga cggtcagatc
     3421 gccgcctact tcaagcacgc ctccgacaag cagctcgaat ggatctcgcc gttcgccagc
     3481 tggatggtcg acaacgccga cgtgcggatc gcaattggcg ccagcaccaa cacgcgtgag
     3541 ctctccgggg tgccgccgga gaagcagatg ctgcgccagt cggtcaccgg cgacctgatg
     3601 cagcgggcga tgaagcgctc ggccgacggc gacttccgct gggtctacac cttgttcccg
     3661 accagcgctt atgcctccga ggcggagatg agcctggccg actacgagga cttctactac
     3721 ggcgcttgcc tggccgatgc cgccgacccg ctgagcgcct ggcgcgaggc ctcgaaggag
     3781 accgaacggc tggccgaatg gatcgacggc cacgaggagg tgcgggtgac cgcgccgggc
     3841 accgacatca agctcgggat cgccggccgc aagttcatcc cctgcgacgg caagcacaac
     3901 atgcccgacg gagagttctt caccggcccg atcgaggact cggtcgaagg cgaagtcagc
     3961 ttccacctgc ccgcggtgat cggcggtcgt gaggtcgccg gcgtcaagct ccgcttcgag
     4021 gccggcaagg tcgtcgacgc aactgccgag cgcggcgagg agttcctgct gaagatgctc
     4081 gacaccgacg acggcgcgcg cacgctcggc gagctcggga tcggcaccaa ctacgggatc
     4141 gatcgcggca cccgcgaggt cctgctcgac gagaagatcg gcggcaccgt gcacatggcg
     4201 gtcggcgcca gctaccccga gtcaggcggc accaacgagt ccgcggtcca caccgacctc
     4261 gtctgcgacc tccgcctcgg cggcaagctc gaagtcgacg gcgtcgtcat gcaggaagac
     4321 ggcaagttcg tcgtctgacc cgtcggtcag gtcagggtga ccagcgccgc gatcgtcccc
     4381 atgaacccca ggaacaccgt tcccaccatc gcgaacatga attgatgaat cgtgcgattg
     4441 agggcgccga tctgccgatg gacctctctg atctcctcgc gaacatctcg gaactctgcc
     4501 cgattttcgg cgaaccctcg atcgaccctg tccttcaggt cgtcgagtct cgcgtcggtc
     4561 caggagtccc tcgccatgac tgccatggtc gcaccgtaac gggacaaatg tcacgtgttg
     4621 tctgcgcaac ttaaaagttg cgcaacatgt tcgtcacgta gtcgaaccgc cccggcgggc
     4681 gacccgccta ctggttcggg atcggtgagc cggagaggaa gaagaaccag aaggtgagtg
     4741 ccagggcggc gagcatcacc gagcccatca gcaccgcctc gaccaccgtc accgaaccgc
     4801 gcacgggagt ggcgtcgcgc atgctgcgca gccaggtcgg ccggatcggt tccagccggt
     4861 tcgtaccggt gatccgcacg tagaggtcgt tgaggcgccc gatcagccag gcgacgacca
     4921 cgaccgagac gaggatcccg acgatcacga ccacataggg gccgccgctc gggaactggg
     4981 tgtgcgagac cttcgagccg atgtagaccc aggtcagcgg aatcgcggtc cagagcgaga
     5041 gcgcggc
//
LOCUS       DASRIG010000482         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3612377, whole genome shotgun sequence.
ACCESSION   DASRIG010000482 DASRIG010000000
VERSION     DASRIG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612377"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..151)
                     /locus_tag="VFB52_12940"
     CDS             complement(5..151)
                     /locus_tag="VFB52_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07287.1"
                     /translation="MPWIWKAGMVILLVCLVASMVIAVYRLSTTPTEILGDGFRGLPA
                     NYKR"
     gene            200..925
                     /locus_tag="VFB52_12945"
     CDS             200..925
                     /locus_tag="VFB52_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07288.1"
                     /translation="MGLEQVADGVWMLRGDFRKAMNIFFIAEDDGSGVVMFDAGTQAM
                     RKKARAAADELGGCRRVVLGHAHCDHRGSAPYMGAPVYCHPDEVADAENDSWRAPYWH
                     LELLPVTPIRWLYERVLLPRWDGGAVKIAGTVAEGDEIASGFQVLHLPGHAPGLIGLW
                     RERDRVALVSDVVYLVDSGRLKPLPHGEASVPHPAWAWNHERARESVRRLAALEPEVV
                     CAGHEEPLRGPGLRETLERAAEK"
     gene            938..>2779
                     /locus_tag="VFB52_12950"
     CDS             938..>2779
                     /locus_tag="VFB52_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07289.1"
                     /translation="MFGTYASAALICVASLLVGRALLTVAGRQTWSWLEPAVGFGAIL
                     TVTGLLARAPGHGTSASVGALLLVIVAAFVVYRERFEAPGALRTGLPVVLVIAIVLAI
                     PFAVSGRWGLIGVGFNNDLGLHLAWAEWLRSGFGPAPDTGYPLGPHGLAVATAVIPGI
                     NLAQAFLGEIVAIGLLTGLTAIGALTEMRPGRRTLAAVLVALPYLAASYFAQAAFKET
                     AEALFVLAFAVFLLAPPALPDGLGPRLRYLLPLLALFGGIFFSYSFAGVAWPIAILAL
                     WSLTLPVVRQALRPRALLRFLAKPLTILGIVVLAGLAVLALVGPFGFGSTFNQVAGSN
                     TYGPVSPIESLGVWWTSNYRLDAVGSAVLPGLTGAIAIAALLVGIAWFVQRRQPTIPI
                     ALGASAALYLLSLPFSGEYSQAKALMIAAPLAMLVAAKPLIWEFPSLARRSGNALLRL
                     GWAVLGVAFVGGAIYSSLLVLRDAPVAPAGHGSELQAFVPLVRGEPVLYAGQDRFAAY
                     ELLGADTHVPLVEFPDEAVTPSPEKPFDTGDAYSPIDFDSFSEKTLNTFPYVVTGRAA
                     WNSKAPRNFKRIAETPSYVLWERTGETAEDRHVLLEGVEAGAFAGCDS"
BASE COUNT          332 a         1010 c          982 g          455 t
ORIGIN      
        1 taactcaacg cttgtaattg gcgggcaggc cgcggaaacc gtcgccgagg atttcggtcg
       61 gggtggtcga gaggcggtag accgcgatca ccatcgaggc gaccaggcaa accagcagga
      121 tcaccattcc tgccttccag atccagggca tggtcaaacc ctagttggac tagccgccgg
      181 ggccggttat ggtcagcgca tggggctgga gcaggtggcg gacggggtct ggatgctgcg
      241 cggggacttc cgcaaggcga tgaacatctt tttcatcgcc gaggacgacg gcagcggcgt
      301 ggtgatgttc gacgccggca cgcaggcgat gcgcaagaag gcgcgcgccg cggccgacga
      361 gctcggcggc tgccgccggg tcgtgctcgg ccacgcccac tgcgaccacc gcggcagcgc
      421 tccgtacatg ggcgcgccgg tctactgcca tcccgacgag gtcgccgacg ccgaaaacga
      481 ctcctggcgt gccccctatt ggcacctgga gctgctgccg gtgacgccga tccgctggct
      541 ctacgaacgg gtgctgctgc cgcgttggga cggcggcgcg gtgaagatcg ccggcacggt
      601 cgccgagggc gatgagatcg cctccgggtt ccaggtcctg cacctgcccg gccacgcgcc
      661 cgggctgatc ggcctctggc gcgagcgcga ccgggtcgcc ctggtcagcg acgtcgtcta
      721 cctggtcgac tcggggcggc tgaaaccgct gccgcacggc gaggccagcg tccctcaccc
      781 cgcctgggcc tggaaccacg aacgcgcgcg ggagtccgtg cgccggttgg cggcgctcga
      841 accggaggtt gtctgcgccg gccacgagga gccgctgcgc ggcccagggc tgcgcgagac
      901 gctcgaacgc gccgctgaga agtaagttcc ctgcctcatg ttcgggactt acgcctcggc
      961 ggcgttgatc tgcgtcgcct cgctgttggt cgggcgcgct ctcctgaccg tcgcggggcg
     1021 gcaaacctgg tcgtggctgg agccggcggt cggcttcggc gcgatcctca ccgtgaccgg
     1081 cctgctggcg cgggcgcccg gccacgggac cagcgccagc gttggcgcgc tgctgctggt
     1141 catcgtcgcc gcctttgttg tctaccgcga gcgctttgag gcgccggggg cgctgcggac
     1201 ggggctgccg gtggtgctgg tgatcgcgat cgtgctggcg atcccgttcg cggtcagcgg
     1261 gcgctggggg ctgatcgggg tcggcttcaa caacgacctc ggcctgcacc tcgcctgggc
     1321 cgagtggctg cgcagcggct tcgggccggc gcccgacacc ggctacccgc tcggcccgca
     1381 cgggctggcg gtggcgaccg cggtgatccc ggggatcaac ctcgcccagg ccttcctcgg
     1441 cgagatcgtc gcgatcggcc tgctcaccgg gctgaccgcg atcggcgccc tgaccgagat
     1501 gcggccgggg cggcgcacgc tcgccgcggt gctcgtcgcc ctcccctacc tcgccgcctc
     1561 ctacttcgcc caggccgcct tcaaggagac cgccgaggcg ctcttcgtgc tcgccttcgc
     1621 cgtcttcctg ctggcgccgc cggcgctgcc tgacgggctc gggccgcgcc tgcgctatct
     1681 gttgccgctg ctggcgctgt tcggcggcat cttcttctcc tacagcttcg ccggggtcgc
     1741 ctggccgatc gcgatcctcg ccctctggag cctcaccctg ccggtcgtgc ggcaggcgct
     1801 gcgaccgcgc gcgctgctgc gtttcctcgc caagccgctg acgatcctcg ggatcgtcgt
     1861 cctcgccggg cttgccgtgc ttgccctggt cgggccgttc ggcttcggca gcaccttcaa
     1921 ccaggtcgcc ggctcgaaca cctacgggcc ggtctcgccg atcgagtcgc tcggggtctg
     1981 gtggacctcg aactaccgcc tcgacgcggt tggctccgcc gtcctgcccg ggctgaccgg
     2041 ggcgatcgcg atcgccgccc tgctcgtcgg catcgcctgg ttcgtgcagc ggcgccagcc
     2101 gacgatcccg atcgcgctcg gcgccagcgc cgccctctac ctgctctcgc tgccgttcag
     2161 cggcgagtac tcacaggcga aggcgctgat gatcgccgcc ccgctggcga tgctggtcgc
     2221 cgccaagccg ctgatctggg agttccccag cctcgcccgc cgctccggca atgcgttgct
     2281 gcgcctgggc tgggcggtgc tcggggtcgc cttcgtcggc ggcgcgatct actcgagcct
     2341 gctggtgctg cgcgacgccc cggtggcgcc ggccgggcac gggtccgagc tgcaggcctt
     2401 cgtgccgctg gtccgcggcg agccggtgct ctacgccggc caggatcgct ttgccgccta
     2461 cgagctgctc ggcgccgaca cccacgtgcc gctggtcgag ttccccgacg aagcggtgac
     2521 gccgagcccg gagaagccgt tcgacaccgg cgacgcctac agcccgatcg acttcgactc
     2581 gttctcggag aaaaccctca acaccttccc ctacgtcgtc accggccgcg ccgcctggaa
     2641 cagcaaggcg ccgcgcaact tcaagcggat cgcggagacg ccgtcctacg tgctctggga
     2701 acggaccggg gagacggccg aggaccgcca cgtgctgctc gagggggtcg aggcgggcgc
     2761 cttcgccggc tgcgactcg
//
LOCUS       DASRIG010000483         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3890074, whole genome shotgun sequence.
ACCESSION   DASRIG010000483 DASRIG010000000
VERSION     DASRIG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890074"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VFB52_12955"
     CDS             <1..1850
                     /locus_tag="VFB52_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZO07290.1"
                     /translation="ASVRVETGEENAERASSEIRESLGFLNTMLLVFAFVAIFVGAFV
                     IFNTFSITVAQRMTEFGMLRTLGASRGQILRTVISESVAIGLLGSLIGIAGGFVIAIA
                     LKGLFEAIGANLPTTGLVLESRTVIVSLLVGVGITLISSLIPALRATKIPPIAALHAE
                     VLPPSRRRRVINAVLSGLLGLAGLAMLAVGLLGGADGDAAAVLMGAGAVAFVIAVSLF
                     SPALVRPLAAVAGWPLERLRRLTGRLARENAQRNPGRTAITAAALMIGLALVAFVTIF
                     AAGLKSSIAEAVDDTFQGDLVIQNSNGFSPIPPAAADAARKVDGVELVATMRAIEAKL
                     VGTGAKPRVNGLSRDAAPVFELEWTQGGEALLRDLRDDQALVKESFAESHDLELGDTF
                     QLLSQAGKKPSFEVVGTYDTKVGLIGGVIVTQDVLARDFEQTQDTIDFVKTAQGADPG
                     QVQKRLETVMERAFPTAEVVNQQELKEKQEEQVNQLLALIYALLALAVLISLFGIANT
                     LALSIHERTRELGMLRAIGMSRRQVRTMIRYEAVITALIGAILGMVLGIIFAVLIAQP
                     LKDEGFTLSFPVGQLILLLVFAAIAGVVAAIPPARRASRLDVLDSLHYE"
     gene            complement(1857..2927)
                     /locus_tag="VFB52_12960"
     CDS             complement(1857..2927)
                     /locus_tag="VFB52_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07291.1"
                     /translation="MEPPHLPPSVEFKLLRYICGLSPRTQRMMFGAPPEIDGQVLASD
                     VHAMLELARRSGARSYTNLLPPVEAREYNSKAAAATRPRKPLRMGRVENMTIPTAAGP
                     LGARFYLPPNLPGGTPPPVLVYYHGGGWVIGDLETHDGVCRFLAAAAGLAVLSIDYRL
                     SPEHPFPAAIEDAWDAFAWAKAQGAELGIDPARVAVGGDSAGGNMAAVVSLRGRDEGR
                     APAMQLLIYPVTDSADDPRSRNLFADGFMLTKADMDLFEGYYVGPGTDINDPRISVFK
                     APDLSGLAPAYVATAGFDPLRDEGEAYAIRMQDAGVPVALKRYPGLIHGFANQTAFSR
                     SSRAAMFEIAGALRMGLAASAS"
BASE COUNT          482 a         1095 c         1151 g          528 t
ORIGIN      
        1 cggcttccgt gcgggtggag accggtgaag aaaacgccga acgcgcctcc agcgaaatcc
       61 gcgaaagcct cggcttcctc aacacgatgc tgctggtctt cgccttcgtc gcgatcttcg
      121 tcggcgcttt cgtcatcttc aacaccttct cgatcacggt cgcccagcgg atgaccgaat
      181 tcgggatgct gcgcacgctt ggcgcctcgc gcggccagat cctgcggacc gtgatctccg
      241 aatcggtggc gatcggcctg ctcggttcgc tgatcgggat cgccggtggc ttcgtgatcg
      301 caattgcgct caaaggcttg ttcgaagcga ttggggccaa cctgccgacc accggtcttg
      361 tcctcgagag ccgcaccgtg atcgtctcgc tgctggtcgg ggtcgggatc acccttatct
      421 cctcgctgat ccccgcactg cgcgcgacca agatcccgcc gatcgcggcg ctgcacgccg
      481 aggtgctgcc gccgagccgc cgccgccgcg tgatcaacgc ggtcctgtcc ggcctgctcg
      541 gcttggccgg gctggcgatg ctggcggtcg gtctgctcgg gggcgccgac ggcgacgcgg
      601 ccgccgtctt gatgggggcc ggcgcggtcg ccttcgtgat cgccgtctct ctcttcagcc
      661 ccgcgctggt gcggccgctc gcggccgtcg ccggctggcc gctggagcgg ctgcgccgac
      721 tgaccggacg cctggcgcgc gagaacgccc agcgcaaccc cggccgcacc gcgatcaccg
      781 cggcagcgct gatgatcggc ctcgctctgg tcgccttcgt gacgatcttc gctgccggcc
      841 tcaagagctc gatcgcggag gcggtcgacg acaccttcca gggcgacttg gtgatccaga
      901 acagcaacgg gttctcgccg attccaccgg cagcggccga cgccgctcgc aaggtcgacg
      961 gcgtcgagct ggtggcgacg atgcgggcga tcgaagcgaa gctggtcggc actggcgcga
     1021 agccccgggt caacggcctt tcgcgtgacg ccgccccggt cttcgagctc gaatggacgc
     1081 agggcggaga ggcgctcttg cgcgacctcc gcgatgacca ggcgctggtc aaagagtcgt
     1141 tcgccgaatc gcacgacctc gaactcggcg acaccttcca gctgctcagc caggccggca
     1201 agaagccgag cttcgaagtc gtcggcactt acgacaccaa ggtcgggctg atcggcgggg
     1261 tgatcgtcac ccaggacgtg ctcgcgcgcg acttcgaaca gacccaggac acgatcgact
     1321 tcgtcaagac cgcgcagggc gccgatcccg ggcaagtcca gaaacgcctg gaaacggtga
     1381 tggaacgcgc gtttccgacc gccgaggtgg tcaaccagca ggaactgaag gaaaaacagg
     1441 aagagcaggt caatcagctg ctcgccttga tctacgcgtt gctggcgctg gcggtgctga
     1501 tctcgctgtt cgggatcgcc aacacgctgg cgctctcgat ccacgagcgc acccgcgagc
     1561 tggggatgct gcgggcgatc gggatgtcgc ggcgccaggt gcggacgatg atccgctacg
     1621 aggcagtgat tacggcgctg atcggggcga tcctcgggat ggtcctcggg atcatcttcg
     1681 cggtcctgat cgcgcagccg ctgaaagacg aaggcttcac gctgagcttc ccggtcgggc
     1741 agctgatcct gctgctggtc tttgcggcga tcgccggcgt ggtcgcggcg atcccgcccg
     1801 cccgccgcgc ctcacggctc gacgtgctcg actcgctgca ctacgagtag ggccgctcag
     1861 gacgcgctgg cggccaggcc catgcgcaac gccccggcga tctcgaacat cgccgcccgc
     1921 gaggagcggc tgaacgcggt ctggttggca aagccgtgga tcagccccgg gtagcgcttc
     1981 agggccaccg gcacgcccgc gtcctgcatg cggatcgcgt aggcctcgcc ctcgtcacgc
     2041 aggggatcga agccggcggt ggcgacgtag gccggcgcga gcccgctcag atccggcgct
     2101 ttgaagaccg agatgcgggg atcgttgatg tcggtgccgg gaccgacgta gtagccctcg
     2161 aagaggtcca tgtcggcctt ggtgagcatg aagccgtcgg cgaagaggtt gcgcgagcgg
     2221 gggtcgtcgg cggagtcggt gaccgggtag atcagcagtt gcatcgccgg cgcccgtccc
     2281 tcgtcccgtc cccgcaggga gacgacggca gccatgttgc cgccggcgct gtcgccgccg
     2341 acggcgaccc gggccgggtc gattcctagt tcggcgccct gcgccttggc ccaggcgaag
     2401 gcgtcccagg cgtcttcgat cgcggccggg aacgggtgct cgggggagag gcggtagtcg
     2461 atcgagagca ccgcgagacc cgccgctgcg gcaaggaagc ggcagacgcc gtcgtgggtc
     2521 tcgagatcgc cgatcaccca gccgccgccg tggtagtaga cgagcaccgg tggcggcgtg
     2581 cctccgggca gattgggcgg caggtagaag cgggcgccga gcggtccggc ggcggtcgga
     2641 atcgtcatgt tttcgacccg tcccatgcgc agcggcttgc gcggccgggt cgcggcggcg
     2701 gctttggagt tgtattcgcg cgcctccacc ggcggcagca ggttggtgta gctgcgggcg
     2761 ccgctgcggc gcgccagctc gagcattgcg tgcacgtcac tggccagcac ctgcccgtcg
     2821 atctcgggcg gggctccgaa catcatgcgc tgggtgcgcg gcgagagccc acagatgtag
     2881 cgcagcagct tgaactcaac gctgggcgga aggtgcggcg gttccacggt cggccagcct
     2941 acgccaaacg gggtccgagc atcacacgat ttggtgtagc cgtgcgtaac cggggggaga
     3001 gtcggcgact cgctgccctt ggtgtcccca tgctgaagca agggatcgca cgtggtgcct
     3061 ggctcatcac cgtggcgccg ggagcgcttt gcgccggtct gctcacgaca ctcctgggcg
     3121 gcgccgcggt gggggagacg aggactgcga ccccgccgac ccggatccag tgcgccacgc
     3181 cggcgaccct gcgcctgcgc cgcttcgagg acggctccgc ccaggtgctt tgcgcccacc
     3241 gcgtgatcgt ccgcat
//
LOCUS       DASRIG010000484         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3612858, whole genome shotgun sequence.
ACCESSION   DASRIG010000484 DASRIG010000000
VERSION     DASRIG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612858"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2632)
                     /locus_tag="VFB52_12965"
     CDS             complement(<1..2632)
                     /locus_tag="VFB52_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZO07292.1"
                     /translation="MATIDKTRALEPEASAPIGWDGQTLAEMLTASESMFEQTGHYHG
                     LRELAMKDSDPLHFEKLFSRVRGGMVTARETALNISASPIVRELGEICFALYTPEGDN
                     VALSTGIIVHVHTMSEAIKFMVRNGWEDNPKIRPGDIFANNQPTIGDVHNADVQTFVP
                     IFNPDNPGELLGWAGGVIHVMDIGASTPGGVPVGPTTRFDDGIDLHCTKIGENDTIAQ
                     WHTKRCSMQSRAPGLYLLDERTRLAGCHMIRDAVERLVLEEGEAPFKQFMREAIEDGR
                     RDFKSRVRGLLVPGRYRSAGFTEAKYENKKNLPKRAQRDLIIHGAYEAVVDRDGRWTT
                     DLRGSSAWGWHSFNATPSAQQGMQWILFTQTLICNDKINDGAYYATELLLTEGSWAHL
                     GEAPCSSSFPWMPMFTTSVGYLRAVSRALQSRGYIEEIVSTYTVPGNVAQGGGLDQYG
                     QISGFMNFEIGAQGQGAKYVLDGLDYGAAVFNPEGDMGDIEMWELVKPMVYLGRRIKP
                     NTGGWGRHRGGSSFETLLLVNGTQDFEIENIGAGGMLTSPGLFGGYPAPSAYVHNVFG
                     SDVFEQAAAGEAYPVGDVSGEEAALNQFAGEHQLEQDPYTMMIAVKTGDLYLSTGRGG
                     GGLGDPLLRPEQAIEEDIAGGHLQRPWAERAYGIEGREGLMHARLDRARPAQEWWAEQ
                     RQRILDQDLIETVKVMYAESMRLEPRWAAEFRGFWDLPEDFDFDVITPTVVGVERAAP
                     GKLTPEESVQEFLSASEVFQPSDQVEVPVTTTVTKETLADLLDEKLSRRAVKDIQSGI
                     KDPDRFEKWIELLQERVDYDDPIVLPYGEGMNIVRRASDGELVIRTDAGADLCRWDEN
                     WKLHVPVFVRDSDELYEEVYP"
     gene            complement(2635..>3019)
                     /locus_tag="VFB52_12970"
     CDS             complement(2635..>3019)
                     /locus_tag="VFB52_12970"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07293.1"
                     /translation="ESIEDVEDLIETFETTYTRMYARAASSPELGYLVAQAIVKGSVP
                     IEHPTLPTMPSESGSPPVKETREVWWPEGPATTDVYEMDDVRNGHTIEGPAVLEAEST
                     TFPIPPDRRAWLDEHGIFHLENKEA"
BASE COUNT          415 a          978 c         1097 g          529 t
ORIGIN      
        1 tggggtagac ctcctcgtag agctcgtcgc tgtcgcggac gaagaccggc acgtggagct
       61 tccagttctc gtcccagcgg cagaggtcgg cgccggcgtc ggtgcggatc accagctcgc
      121 cgtcgctggc gcggcggaca atgttcatgc cttccccgta ggggagcacg atcgggtcgt
      181 cgtagtcgac ccgctcctgg agcagctcga tccacttctc gaagcggtcg gggtccttga
      241 tccccgactg gatgtccttg acggcacggc gggagagttt ctcgtcgagc aggtcggcca
      301 gcgtctcctt ggtgaccgtg gtggtcaccg ggacttcgac ctggtcggag ggctggaaca
      361 cctcggaagc tgagaggaac tcctgcacgg attcctcggg ggtcagcttg ccgggggcgg
      421 cgcgctcgac gccgacgacg gtcggggtga tcacgtcgaa gtcgaagtcc tcgggcaggt
      481 cccagaagcc gcggaactcg gcggcccagc gcggttcgag ccgcatcgac tccgcgtaca
      541 tcaccttcac ggtctcgatc aggtcctggt cgaggatgcg ctggcgctgc tcggcccacc
      601 actcctgggc ggggcgggcg cggtcgaggc gggcgtgcat cagaccttcg cggccctcga
      661 tgccgtaggc ccgctcggcc cagggccgct gcaggtggcc gccggcgatg tcttcctcga
      721 tcgcctgctc ggggcgcagc agcgggtcgc cgaggccgcc gccgccgcga ccggtcgaga
      781 ggtagaggtc gccggttttg accgcgatca tcatcgtgta ggggtcctgt tcgagctggt
      841 gctcgccggc gaactggttc agcgccgcct cctccccgct gacgtcgccg accgggtatg
      901 cctcgcccgc cgccgcctgc tcgaagacgt ccgagccgaa gacgttgtgg acgtaggcgc
      961 tgggcgccgg gtagccgccg aacaaccccg gggaggtcag catgcccccg gcgccgatgt
     1021 tctcgatctc gaagtcctgg gtgccgttga cgagcagcag cgtctcgaac gaggagccgc
     1081 cgcggtggcg accccagccg ccggtgttcg gcttgatccg ccggccgagg tagaccatcg
     1141 gcttgaccag ctcccacatc tcgatgtcgc ccatgtcgcc ctcggggttg aacacggcgg
     1201 cgccgtagtc gagcccgtca aggacgtact tggcgccctg gccctgggcc ccgatctcga
     1261 agttcatgaa gccggagatc tgcccgtact ggtcgagccc gccgccctgg gcgacgttgc
     1321 cgggcaccgt gtaggtggag acgatctcct cgatgtagcc gcgcgactgc agagcgcgcg
     1381 agaccgcgcg caggtagccg accgaggtcg tgaacatcgg catccagggg aaggacgagg
     1441 agcagggtgc ctcgccgagg tgggcccagc tgccctcggt gagcagcagc tcggtcgcgt
     1501 agtaggcgcc gtcgttgatc ttgtcgttgc agatcagcgt ctgggtgaag aggatccact
     1561 gcatgccctg ctgcgccgag ggggtggcgt tgaaggagtg ccagccccag gccgaggagc
     1621 cgcgcaggtc ggtggtccag cggccgtcgc ggtcgaccac ggcctcgtag gcgccgtgga
     1681 tgatcaggtc gcgctgggcg cgtttgggca ggttcttctt gttctcgtat ttggcctcgg
     1741 tgaagccggc ggagcggtag cggccgggga ccaggaggcc gcggacgcgg gacttgaagt
     1801 cgcggcggcc gtcctcgatc gcctcgcgca tgaactgctt gaacggcgcc tcgccttctt
     1861 cgagcacgag ccgctcgacc gcgtcgcgga tcatgtggca gccggccagg cgggtgcgct
     1921 cgtcaagcag gtagagaccc ggggcgcggc tctgcatcga gcagcgcttg gtgtgccact
     1981 gggcaatcgt gtcgttctcg ccgatcttcg tgcagtgcag gtcgatgccg tcgtcgaagc
     2041 gggtggtcgg gccgaccggg acgccgcccg gggtgctggc gccgatgtcc atcacgtgga
     2101 tcacgccgcc ggcccagccg agcagctcgc ccgggttgtc gggattgaag atcggcacga
     2161 aggtctgcac gtcggcgttg tggacgtcgc cgatcgtcgg ctggttgttg gcgaagatgt
     2221 cgccgggccg gatcttcggg ttgtcctccc agccgttccg caccatgaac ttgatcgcct
     2281 cgctcatcgt gtggacgtgg acgatgatcc cggtcgagag cgcgacgttg tcgccctcgg
     2341 gggtgtagag ggcaaagcag atctccccga gctcgcggac gatcggcgag gcgctgatgt
     2401 tgagcgcggt ctcgcgggcg gtgaccatgc cgccgcgcac ccgcgagaac agcttctcga
     2461 agtgcagcgg gtcggagtcc ttcatcgcca gctcgcgcag gccgtggtag tggccggtct
     2521 gctcgaacat gctctcggag gcggtcagca tctccgccag cgtctgcccg tcccagccga
     2581 tcggcgccga ggcctcgggc tcgagggcgc gggtcttgtc gatcgtggcc atcgtcaggc
     2641 ctccttgttc tcgaggtgga agatgccgtg ctcgtcgagc caggcgcggc ggtcgggcgg
     2701 gatcgggaag gtcgtcgact cggcctcgag caccgccggg ccctcgatcg tgtggccgtt
     2761 gcggacgtcg tccatctcgt agacgtcggt ggtcgctggt ccctcgggcc accagacctc
     2821 gcgggtctcc ttgaccggcg gcgagccgga ctcgctcggc atcgtcggca gggtcggatg
     2881 ctcgatcggg accgagccct tgacgatcgc ctgcgcgacc aggtagccga gctccggcga
     2941 ggaggcggcc cgcgcataca tccgcgtgta ggtggtctcg aaggtctcga tcagatcttc
     3001 gacgtcctcg atcgactcg
//
LOCUS       DASRIG010000485         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3325891, whole genome shotgun sequence.
ACCESSION   DASRIG010000485 DASRIG010000000
VERSION     DASRIG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325891"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1307)
                     /locus_tag="VFB52_12975"
     CDS             complement(231..1307)
                     /locus_tag="VFB52_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZO07294.1"
                     /translation="MLLAVLGLAVGVAACGSDDDSSSSGSSGSAESTESTDSGEVSID
                     LGDKTVEVPAGTKPKIGFLAYGLSAYELSYAEEIEKMNGEGHDVVWVEAKYDAATQLK
                     QLQSAITEETYDAMVIESVDAEGACRIVSQQAPEAGIPVGIIINPVCGRALNPWGEEV
                     WAPGTLNVVSDTANVTYFTNVLKVQQEELGIGPDTKVGVINGPDIAAQAQSYGNAIEA
                     SGIKPTEMVAGDYTAPTAQKLVAAMLSRNPDIEVILTGYEGATPGIISALKAAGKSPG
                     EVKVGDVGGSSEISVPNIKSGWLEISAVYDPKKIARTAVEEMEKAFEGEQGPRFLTAD
                     PVGGTLEKPLQLTKANVGSFEPTY"
     gene            complement(1362..>2048)
                     /locus_tag="VFB52_12980"
     CDS             complement(1362..>2048)
                     /locus_tag="VFB52_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07295.1"
                     /translation="EHGAEVTMLQRGATYVVTFETFNKYWFGLYTEEQPLPIELADQV
                     AYSAPNLIGDEINKQLVELAKEEDKELLDALESQGFKLEWGPEGTGIIGAHMAGRDSY
                     QIDIGASALVADGRVKLKSGAELAEVRGKTAIFTDGSEADFDLILFATGYEQLWDYMG
                     PALGIADKIPKVYGRAEDNEYANVWRRSAQPGLWFGTGFIGMARFHSKFMTLLIKAIE
                     EGIAPVDPDK"
BASE COUNT          279 a          665 c          682 g          422 t
ORIGIN      
        1 gtcgacttgc cggcgccgtt ctcgccgacc agggcgtgca cggtgccgcg gcggatcgtc
       61 aggtcgaccg agtcgagcgc gacggtcacc ccgaaggtgc ggcgcacccc gtggaactcg
      121 atcgtctctg cgctcatcgc gacccgtccg cttctgcctg gtggggggag gtcatgcgtg
      181 gtcgggagcg gcggcgccgg ctatcgaggg tccggcgccg ccttcccggg tcagtacgtc
      241 ggttcgaagc tgccgacgtt cgctttggtc agctggagcg gtttctccag cgtgccgccg
      301 accggatccg cggtgaggaa gcgcgggccc tgttcgcctt cgaacgcttt ctccatctct
      361 tcgaccgcgg tgcgggcgat tttcttcggg tcgtagaccg cgctgatctc cagccagccc
      421 gatttgatgt tcgggaccga gatttcgctc gagccgccga cgtcgccgac ttttacctcg
      481 cccgggctct tgccggccgc tttcagcgcc gagatgatcc ccggggtcgc accctcgtaa
      541 ccggtgagga tgacttcgat gtcggggttg cgggagagca tcgccgcgac cagtttctgg
      601 gcggtcggcg cggtgtagtc gccggcgacc atttcggtcg gtttgatccc ggaggcctcg
      661 atcgcgttgc cgtaggactg cgcctgggca gcgatgtcgg ggccgttgat cacgccgact
      721 ttggtgtccg gaccgatccc gagttcttcc tgctggactt tcaggacgtt ggtgaagtag
      781 gtcacgttcg ccgtgtcgga gacgacgttg agcgtccccg gggcccagac ttcttcgccc
      841 cacgggttga gcgcgcgacc gcagaccggg ttgatgatga tcccgaccgg gattcccgct
      901 tccggggcct gctgggagac gatccggcag gcgccttcgg cgtcgacgga ctcgatcacc
      961 atcgcgtcgt aggtttcttc ggtgatcgcc gactgcagct gtttgagctg ggtcgccgcg
     1021 tcgtacttgg cttcgaccca gacgacgtcg tggccttcgc cgttcatttt ttcgatctcc
     1081 tcggcgtagg agagctcgta ggccgagagg ccgtaggcga ggaagccgat tttcggtttc
     1141 gtcccggccg gcacctcgac cgtcttgtcg ccgaggtcga tgctgacttc gccggagtcc
     1201 gtggattccg tgctttcggc cgagccactc gagccggagc tggagctgtc gtcgtcacta
     1261 ccgcaggccg caactccaac cgccaaccca aggacggcca gaagcacggc acaagccttc
     1321 agcaggctct tcattcgttc cctctctcct cgttcgtagg actacttgtc gggatcgacg
     1381 ggggcgatgc cctcctcgat cgccttgatc agcaaggtca tgaatttcga gtggaagcgg
     1441 gccatgccga tgaacccggt cccgaaccag aggcccggct gggcggagcg ccgccagacg
     1501 ttcgcgtact cgttgtcctc ggcgcggccg tagaccttcg ggatcttgtc ggcgatgccg
     1561 agcgcggggc ccatgtagtc ccagagctgc tcatagccgg tcgcgaacag gatcaggtcg
     1621 aagtcggctt ccgagccgtc ggtgaagatc gccgtcttgc ccctgacctc ggcgagttcg
     1681 gcgccgctct tcagcttgac ccggccgtcg gcgaccagcg ccgaagcgcc gatgtcgatc
     1741 tggtagctgt cgcggcccgc catgtgggcg ccgatgatcc ccgtgccttc cgggccccat
     1801 tcgagcttga agccctggga ctcgagcgcg tcgagcagct ccttgtcctc ctctttggcg
     1861 agctcgacca gctgcttgtt gatctcgtcg ccgatcaggt tcggggccga gtaggcgacc
     1921 tggtcggcga gctcgatcgg cagcggctgc tcctcggtgt agaggccgaa ccagtacttg
     1981 ttgaaggtct cgaaggtgac cacgtaggtg gcgccgcgct gcaacatcgt cacctcggcg
     2041 ccgtgctc
//
LOCUS       DASRIG010000486         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1782451, whole genome shotgun sequence.
ACCESSION   DASRIG010000486 DASRIG010000000
VERSION     DASRIG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782451"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFB52_12985"
     CDS             complement(<1..683)
                     /locus_tag="VFB52_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZO07296.1"
                     /translation="MTDETLRNQPRSLFDGPIMVRAALDSLRKLDPRHQFRNPVMFVV
                     EIGALITTIGWLVQVFGGSPLGGGDDPAWFSFAIAFWLWLTVVFANFAEALAEGRGKA
                     QADALRSMRTETVANLRDGGSKPASELRRGDVVVVEAGETIPGDGTVIEGIASVDESA
                     ITGESAPVVRESGGDRSAVTGGTRVLSDRIVVEVTQEPGQSFLDKMIALVEGAERRKT
                     PNEIALNILL"
     gene            complement(680..1273)
                     /gene="kdpC"
                     /locus_tag="VFB52_12990"
     CDS             complement(680..1273)
                     /gene="kdpC"
                     /locus_tag="VFB52_12990"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZO07297.1"
                     /translation="MNGLRKDLTTAAIVLVFFTLLLGIAYPLVTTGVAQVLFPGAADG
                     SKIEVDGKVVGSELIGQDFSGQPRYFQGRPSATEYSANVTYFNNLGPNNKELSEFFEE
                     ELDAYVQREGRYDKGLTADDVPADAVTTSASGVDPHISEANARIQAHRVAAVRGLPLE
                     RVEELIEANTDGRFIVFGEPGVNVLKLNIALDQEAAK"
     gene            complement(1290..3020)
                     /gene="kdpA"
                     /locus_tag="VFB52_12995"
     CDS             complement(1290..3020)
                     /gene="kdpA"
                     /locus_tag="VFB52_12995"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZO07298.1"
                     /translation="MAQGWLQFAVFIVLLTALVPLLGTYLAKVFQGERVFLSPVVGPI
                     ERLSYRLFRVRPGDGQDWKAYARSVLVFSLLSWLLLYLILRTQSVQPFNPEGFGSGPW
                     NLSFNTTSSFVTNTNWQYYGGETTLSYFAQMAGLAVQNFVSAAVGIAVLAAVIRGFAA
                     RSASSLGNFWSDLTRITLYVLLPISFVAALVLVSQGVVQTLSGSELVHTLTGVEQTLS
                     FGPVASQEAIKELGTNGGGFFNVNSAFPFENPNNFTNILEIFLMLSIPASLPYAFGRI
                     IGNRRQGWAIYAAMMLMFVVSVAVVFIAEQHGSPAQHAAGVLGGNLEGKEVRNGIPQS
                     SLWTAVTTVTSCGAVNAAFDSLTGIGGLMPTANLSSGEVIFGGVGSGLYGMLMLVLLT
                     VFIAGLMVGRTPEFLGKKIEAREVKLVLVAVLATPLLVLVGTAWAVASSDGTMSIYNP
                     GPQGFSESLYAYMSQANNNGSAFAGYTGFLQPNAPGNGGSEAFDFANLLGGLVMLFGR
                     FLPLIAVLAVAGSLAKKKVAPVGAGTFRADSPTFVVLLLGVVVIVAALTFFPAFLLGP
                     VVQGLTDQLF"
     gene            complement(3180..>3492)
                     /locus_tag="VFB52_13000"
     CDS             complement(3180..>3492)
                     /locus_tag="VFB52_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZO07299.1"
                     /translation="PEGAEPTIVADGLEIDLAGRTVRRDGEPVHLTPTEFELLRELAR
                     NRGRLLTHRALLVAVWGVEYEDDFQVLRAHIANLRRKIEPPDGPKYVLTDPGVGYRFA
                     A"
BASE COUNT          592 a         1104 c         1275 g          521 t
ORIGIN      
        1 agcaggatgt tgagggcgat ctcgttcggc gtcttgcgcc gctcggctcc ctcgaccagg
       61 gcgatcatct tgtcgaggaa gctctgtccg ggctcctggg tcacctcgac cacgatccgg
      121 tccgagagca cccgggtgcc gccggtgacc gcggatcgat cgccgccgga ctcgcgtacg
      181 accggcgccg actcgccggt gatcgccgac tcgtcgaccg aggcgatccc ctcgatcacg
      241 gtgccgtcgc cggggatcgt ctcccccgcc tcgaccacga ccacgtcgcc gcgccgcagc
      301 tcggaggcgg gcttgctgcc gccgtcgcgc aggttggcaa ccgtctcggt ccgcatcgat
      361 cgcaacgcgt cggcctgcgc cttgccgcgg ccctcggcca gggcctcggc gaagttggcg
      421 aagacaacgg tcagccaaag ccagaaggcg atcgcgaacg agaaccaggc cgggtcgtcg
      481 ccgccgccga gcggggagcc gccgaagacc tggaccagcc agccgatcgt cgtgatcagg
      541 gcgccgatct caaccacgaa catcaccggg ttgcggaact ggtggcgggg gtcgagcttg
      601 cgcagcgagt caagcgccgc ccgaaccatg atcgggccgt cgaacaacga gcgcggctgg
      661 ttgcggaggg tctcgtcggt cactttgcgg cctcctggtc aagggcgatg ttgagcttca
      721 gaacgttgac cccgggctca ccgaagacga tgaagcggcc gtcggtgttg gcctcgatca
      781 actcttcgac ccgctcgagc ggcagaccgc ggacggcggc gacccggtgg gcctggatcc
      841 gggcgttggc ctcggagatg tgagggtcga ccccggaggc cgaggtggtg accgcgtcgg
      901 cgggcacgtc gtcggcggtc aggcccttgt cgtagcggcc ctcccgctgc acgtaggcgt
      961 cgagttcttc ttcgaagaat tccgacagtt ccttgttgtt cgggccgagg ttgttgaagt
     1021 aggtgacgtt ggcgctgtat tcggttgccg aggggcggcc ctggaagtag cgcggctggc
     1081 cgctgaagtc ctgcccgatc agctccgagc cgaccacctt gccgtcgacc tcgatcttgc
     1141 tgccgtcggc cgcgcccggg aacaggacct gggcgactcc ggtggtcacc agcgggtagg
     1201 cgatcccgag cagcagggtg aagaagacga ggacgatcgc cgcggtggtg aggtctttgc
     1261 gcagtccgtt catcagaagc tgtccctggt cagaagagtt ggtcggttag tccctgcacg
     1321 accgggccga gcaggaaggc ggggaagaag gtgagcgcgg cgacgatcac gacgacgccg
     1381 agcaggagga cgacgaaggt cggcgagtcg gcccggaagg tcccggcccc gaccggcgcg
     1441 actttcttct tcgccagcga gccggccacc gcaagcacgg cgatcagcgg cagaaaacgg
     1501 ccgaacagca tcaccaggcc gccgagcagg ttggcgaagt cgaatgcttc cgagccgccg
     1561 ttacccgggg cgttgggctg caggaagccg gtgtagccgg cgaaggcgga gccgttgttg
     1621 ttcgcctgcg acatgtaggc gtagagcgac tccgagaagc cctgcgggcc ggggttgtag
     1681 atcgacatcg tgccgtcgct gctcgccacc gcccaggccg tgccgacgag cacgagcagg
     1741 ggcgtcgcca gcaccgcgac caggaccagc ttcacctcgc gcgcctcgat cttcttgccg
     1801 aggaactcgg gggtgcggcc gaccatcagg ccggcgatga agaccgtgag caggaccagc
     1861 atcagcatcc cgtagagccc ggagccgacg ccgccgaaga tcacctcgcc gctcgagagg
     1921 ttggcggtcg gcatcagacc gccgatcccg gtcagcgagt cgaaggcggc gttgacggcg
     1981 ccgcaggagg tgaccgtggt caccgcggtc cacagcgagg actgcgggat cccgttgcgc
     2041 acctccttgc cctcgaggtt gccgccgagg acgccggccg cgtgctgggc ggggctgccg
     2101 tgctgctcgg cgatgaacac gaccgcgacc gagacgacga acatcagcat catcgccgcg
     2161 tagatcgccc agccctggcg gcggttgccg atgatccggc cgaacgcgta gggcaggctg
     2221 gccgggatcg agagcatcag gaagatctcg aggatgttgg tgaagttgtt cgggttctcg
     2281 aacgggaagg cgctgttgac gttgaagaag ccgccgccgt tggtgccgag ctccttgatc
     2341 gcctcctgcg aggcgaccgg gccgaaggac agggtctgtt cgactccggt cagggtgtgg
     2401 accagctcgg agcccgagag cgtctgcacg accccctggg agacgaggac cagcgccgcg
     2461 acgaaggaga tcggcagcag cacgtagagg gtgatccggg tgagatccga ccagaagttg
     2521 ccgagcgagc tggcgctgcg ggcggcgaag ccgcggatca ccgccgccag caccgcgatc
     2581 ccgaccgcgg cggagacgaa gttctggacc gcgagcccag ccatctgggc gaagtagctg
     2641 agcgtcgttt cgccgccgta gtactgccag ttggtgttgg tgacgaacga ggaggtggtg
     2701 ttgaagctga ggttccaggg gccggagccg aagccctcgg ggttgaacgg ctggaccgac
     2761 tgggtgcgga gtatcaggta gagcagcagc caggagagca ggctgaagac gagcaccgag
     2821 cgggcgtagg ccttccagtc ctgcccgtcg ccgggccgca cgcggaacag ccggtagctg
     2881 aggcgctcga ttggcccgac gacgggagac aggaagaccc gctcgccctg gaagaccttc
     2941 gccaggtagg tgccgagcag cggcaccagc gcggtcagca ggacgatgaa gacggcgaac
     3001 tggagccagc cctgcgccat cagacgcggt ccagtgcgtc gattacgaag tagagaatcg
     3061 cgaaggcgat gagggccaga gcgattgaga cgaggtccat gcctctactt tgcgccgccc
     3121 cccgtcaagg ccgcatgaag ttcagccccg cccgtataca gatcctgtga aggtgtcttc
     3181 taggcggcga agcggtagcc gacgccgggg tcggtgagca cgtatttcgg gccgtcgggg
     3241 ggctcgatct tgcggcgcag gttggcgatg tgggcgcgca ggacctggaa gtcgtcttcg
     3301 tactcgaccc cccagaccgc gaccaggagg gcgcggtggg tgagcaggcg gccgcggttg
     3361 cgggcgagct cacgcagcag ctcgaactcg gtcggggtga ggtggaccgg ctcgccgtcg
     3421 cggcgcacgg tgcggccggc gaggtcgatc tcgaggccgt cggcgacgat cgtcggctcc
     3481 gccccctccg gc
//
LOCUS       DASRIG010000487         7647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1532056, whole genome shotgun sequence.
ACCESSION   DASRIG010000487 DASRIG010000000
VERSION     DASRIG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532056"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1193)
                     /locus_tag="VFB52_13005"
     CDS             complement(186..1193)
                     /locus_tag="VFB52_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZO07300.1"
                     /translation="MPSLPKPRLRRHRGDQLVTPPEPGERKPRVETVQGSGLRWVHIE
                     RPTPIECAWLEEHFDFHPLDLEDVLSRNQRPKIDEYPSYLFIVLHFPVFDPQVKRLNA
                     GELDIFVGPDYLVTIPNTPLQPIEYLFERCRQKEELREQLFSRGSGYLLYRVVDDLFD
                     YCFPMLRKIGNKLDALETEIFEESRSEDIVRDISNAKQEIINFRKVIRPQRTVLRDLE
                     NLEQPFLASREGELDVYFDDIGDSHERIWDMLENYKEVVEALEETNESYISHRVNDIL
                     RVLTAFSVVILPLTLIASLWGMNVAVPGEGDPGDFWLIVGGMVLLLVGMLAYFRHRRW
                     L"
     regulatory      1311..1424
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1504..2748
                     /gene="thrC"
                     /locus_tag="VFB52_13010"
     CDS             1504..2748
                     /gene="thrC"
                     /locus_tag="VFB52_13010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZO07301.1"
                     /translation="MAPKSLSCKECGTHYPLEALYVCEQCFGPLEVAYDHSALDPAEA
                     KRKIQAGPAGIWRYADFLPFAGRPRDPLQPGLTPLVCADRLAERLGLTEVWIKNDAAN
                     PTHSFKDRVVAVALAKARELGFETVACASTGNLANAVAAHAAAAGLDSYVFVPTDLEE
                     QKLLATDVYGTTLVGVRGNYDDVNRLCTELCETRPWAFVNVNLRPYYSEGSKTVAYET
                     VEQLGWSLPDRIVCPIASGSLFTRIGKGLQEWLELGLVAGELPAFNGAQPDGCSPVAE
                     AYANDWDVFRPQRPETIAKSLAIGNPADGIYALDLARRTGGAIESVSDAEIRAGIRLL
                     AETTGIFTETAGGVTTAVLAKLADAGAIGRDERVVAIITGEGLKTLDAVRDGFRIEQI
                     EPTVESFEAEVAATASVGAAAS"
     gene            2776..3054
                     /locus_tag="VFB52_13015"
     CDS             2776..3054
                     /locus_tag="VFB52_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZO07302.1"
                     /translation="MAITVKIPAQLRAVTEGEGEIEVEGETVGEALDAVFDSHQDLRE
                     RITEDGTLRRFVNVYVSGEDIRFKDGLETELADGDEVTILPAVAGGRR"
     gene            3041..3796
                     /locus_tag="VFB52_13020"
     CDS             3041..3796
                     /locus_tag="VFB52_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZO07303.1"
                     /translation="MDGANDAADAPVAILCGGRGTRLQEKTHSIPKALVEIGGKPILW
                     HVIRIYAAQGHRRFLLLAGYLREQVEAFVARAQWEEPIAVECLDTGLDTNTGGRVARA
                     ADRLRAGTFCLTYADGVADIDLASQAAFHRRQEALVTMTVVRPYSQWGIALIDEQERI
                     TGFREKPRLDYWINGGFFVCEPAFLDHVGPDSVLERVPLELAAASGRLAAYRHEGFWD
                     CMDTYKDAVTLNDLWQSGRAPWRVWERAGVEAG"
     gene            3793..4767
                     /locus_tag="VFB52_13025"
     CDS             3793..4767
                     /locus_tag="VFB52_13025"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZO07304.1"
                     /translation="MSVALVTGARGFAGAWLARALLERGDRVVSLDKGRPERPASTLS
                     LLGIEADVIDVVGDLCDGDLVRSVLSENRVTDVYHLAAEPIVGAFAASPLRGFETNVR
                     GTWTLLDACLAEAAERVVVASSDKAYGAHETLPYREDFALNATAPYEASKAAADVIAR
                     SYWHSYGLPVAVTRFANIYGGGDLNFSRLVPEAVCAALDGRPPVLRSDGSPERDFLYV
                     EDAAAAYLAIADELDRDEVRGEAFNAGDGRPYRVGEVVETIARLSGTGVEPEIRGEGN
                     PAGEIDRQFVDPSKLRTRCGWEPSVTLEAGLERTIDWYREHPGVRPAG"
     gene            4864..5718
                     /locus_tag="VFB52_13030"
     CDS             4864..5718
                     /locus_tag="VFB52_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZO07305.1"
                     /translation="MRDEHRTGGNLVRAAPIALLGTIVALAAWPQTAPAAVTHVVQPG
                     ESLWGIAVANNLTTRTVAVFNGLPEDALLIAGESIEVPTVDEGAAALASAGTASTAVS
                     GAGTDHLVQPGESLWSIASANGLTVQEVASFNGLAEDALLIAGETIQVPGVSSGTSAG
                     TTVDPSTGLAYLWSPEGSVPLDPAAAASFEQMRQAALSQFGIDLYPAGLLSGYRTYEQ
                     QAYLYDLYLSGQGAPANPPGTSSHEYGAAVDLADPAMRTVVDQIGGVYGWAATIPSEW
                     WHVAYVGG"
     gene            5728..6009
                     /locus_tag="VFB52_13035"
     CDS             5728..6009
                     /locus_tag="VFB52_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HZO07306.1"
                     /translation="MNDAPTDVQDDARKQAIRRLKAKRDFKSFLGTAAIAIVVVIVVW
                     ALSDSDYFWPIWVILGLGIALFFSGLQAYGPHRGPITEAEIQREIDKGT"
     gene            complement(6027..6425)
                     /locus_tag="VFB52_13040"
     CDS             complement(6027..6425)
                     /locus_tag="VFB52_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07307.1"
                     /translation="MSQENIELIRQVYAAVARNDLEAASDGLHPEIEFHTYANAPAAG
                     VYRGRDAVRSYNEDLFAQFESVRLEVDEVVDAGQRVVVMSTQHAVPKGGRQEISVQLV
                     EVWTIRDGRLAERRSYSTRAEALEAAGRSD"
     gene            complement(6540..6932)
                     /locus_tag="VFB52_13045"
     CDS             complement(6540..6932)
                     /locus_tag="VFB52_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07308.1"
                     /translation="MSQENVELVREGFDAFQEGDLTRMLDLMAEDLVTYRADPDGMTY
                     HGKEGFLSATADWTEDFSEWSVTPEEFIDAGERVLVRVRQTARGEASGAPVEGDFWFL
                     FVMRGGRVAKVSFYIRRDDALGAAGLRE"
     gene            complement(6936..7334)
                     /locus_tag="VFB52_13050"
     CDS             complement(6936..7334)
                     /locus_tag="VFB52_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07309.1"
                     /translation="MSEENVERLRAVYSAIATGELALLFELCDPNIEVTEPPEIPDSS
                     THHGHDGIRAVFGKLQEVFPDMEFAAAEFIANGDRVLASMQWLGTGAGSGASARVPLF
                     HVWSFEDGRVTRIRAFLDRRQALEAAGLSG"
BASE COUNT         1226 a         2637 c         2623 g         1161 t
ORIGIN      
        1 cggcgacgac cgtccggccc ggccggccga ccatccacag cacgcccgcc tcggcctcgt
       61 ccatatccgg ctcgtaggca tacgtctcgc cggcctcgac gatcccgcgg aagaacggcc
      121 agatcgccgg ccaatcggcg gcggtcgcct cgcggatctg cacggtacga gcgccgacgg
      181 cgccgctaca gccagcggcg gtggcggaag taggccagca tcccgaccag gagcaggacc
      241 atcccgccga cgatcaacca gaagtcgccg ggatcgccct cgcctggcac ggcgacgttc
      301 atcccccaca ggctcgcgat gagggtcagc ggcaggatca cgacgctgaa cgccgtcagc
      361 acccggagga tgtcgttcac gcggtgcgag atgtaggact cgttcgtctc ctcgagggcc
      421 tcgacgacct ccttgtagtt ctcgagcatg tcccagatcc gctcgtggct gtcgccgatg
      481 tcgtcgaagt agacatccaa ctcgccctcg cgggatgcca ggaacggctg ctcgaggttc
      541 tcgaggtcgc ggagcacggt gcgctgcggc cggatcacct tgcggaagtt gatgatctcc
      601 tgcttggcgt tggagatgtc gcgaacgatg tcctccgacc ggctctcctc gaagatctcc
      661 gtttcgagcg cgtcgagctt gttcccgatc ttgcgcagca tcggaaagca gtagtcgaag
      721 agatcgtcca cgacccggta gagcaggtag cccgatccgc gggagaagag ctgctcgcgc
      781 agctcctcct tctggcggca gcgctcgaac aggtactcga tcggctgcaa cggcgtgttc
      841 gggatcgtga ccagatagtc cgggccgacg aagatgtcca gctcacccgc gttgaggcgc
      901 ttcacctgcg ggtcgaagac cgggaagtgc aggacgatga agaggtagct cgggtactcg
      961 tcgatcttgg ggcgctggtt gcgcgacagc acgtcctcga ggtcgagcgg gtggaagtcg
     1021 aaatgctcct cgagccaggc gcactcgatc ggggtcgggc gctcgatatg gacccagcgc
     1081 aggccggaac cctggaccgt ctcgacgcgc ggcttgcgct cgccgggctc aggcggcgtg
     1141 acgagctggt cgccgcggtg gcggcgtagc ctcggcttcg gaaggctcgg catcggtgct
     1201 cgtctcgctc atcagctcgc tcatcggggt gcgcagaaca gggtacgcga cgcccggccg
     1261 agccgcgcaa tccacgtcgc accactgcta tattcccgcg ccacataacc actgcatcca
     1321 gaggggtgga gggactggcc cgatgaagcc ccggcaacca tcgagggtcg tacgacccga
     1381 gaaaggtgcc aattccagca ggtgacgaac ctgggagatg tgagtggaag tccggtccgg
     1441 acctctgctc gcaacacgca gaggtctttt tcgtagtcga acgaaagccg gaaggagctt
     1501 cagatggcac cgaagtcgct cagttgcaag gagtgtggga cccactaccc gctcgaggcc
     1561 ctctacgtct gcgagcagtg cttcggaccg ctcgaggtcg cctacgacca ctcggcgctc
     1621 gacccggcgg aggcgaagcg caagatccag gcgggaccag ccgggatctg gcgctacgcg
     1681 gacttcctgc cctttgccgg gcgaccccgc gatccgctcc agccagggct gacgccgctg
     1741 gtttgcgccg accggctcgc ggagcgcctc ggcctgaccg aggtctggat caagaacgac
     1801 gccgcgaacc cgacgcactc gttcaaggac cgcgtcgtcg cggtcgccct ggccaaggcg
     1861 cgagagctcg ggttcgagac cgtcgcctgc gcgtcgaccg gaaacctcgc caacgccgtc
     1921 gcggcacacg ccgcggccgc cggcctcgat tcctacgtct tcgtgcccac cgacctcgag
     1981 gagcagaagc tgctcgcgac cgacgtctac gggaccacac tggtcggggt acggggcaac
     2041 tacgacgacg tcaaccgcct ctgcacggag ctctgcgaga cgcggccgtg ggcgttcgtg
     2101 aacgtcaacc tgcgtccgta ctactccgag ggctcgaaga cggtcgccta cgagaccgtc
     2161 gagcaactcg gctggtcgct gcccgaccgg atcgtgtgcc cgatcgcctc cggctcgctg
     2221 ttcacgcgga tcggcaaggg gttgcaggag tggctcgagc tcggcctcgt cgcaggcgag
     2281 ctgccggcct tcaacggggc ccagccggat ggctgcagcc cggtcgccga ggcctacgcg
     2341 aacgactggg acgtcttcag gccgcagcgc ccggagacga tcgcgaagag cctcgcgatc
     2401 ggcaacccgg cggacgggat ctacgcgctc gacctggcgc gccggaccgg gggcgcgatc
     2461 gagtcggtga gcgatgccga gatccgcgcc ggcatccgtc tgctcgccga gaccacgggc
     2521 atcttcaccg agaccgccgg gggcgtgaca acggcggtgc tggcgaagct cgccgacgcc
     2581 ggcgcgatcg gccgcgatga gcgcgtggtc gcgatcatca ccggcgaggg cctgaagacc
     2641 ctggacgccg tgcgcgacgg cttccggatc gagcagatcg agccgaccgt ggagtcgttc
     2701 gaggcggagg tcgcagcgac cgcatcggtc ggcgccgccg caagctaggc cgggtcgggt
     2761 tacatttccg gccgcatggc gataacggtc aagatcccgg cacagctgcg cgcggtcacc
     2821 gagggcgagg gggagatcga ggtcgagggc gagacggtcg gcgaggcgct cgacgcggtc
     2881 ttcgactcgc accaggacct gcgcgagcgg atcaccgagg acggcacgct gcggcggttc
     2941 gtaaacgtct acgtctcggg cgaggacatc cgcttcaagg acggcctcga gaccgagctc
     3001 gccgacgggg acgaggtcac gatcctgccc gccgtcgccg gtggacggcg ctaacgacgc
     3061 ggccgacgcc ccggtcgcaa tcctctgcgg cgggcgcggc acgaggctgc aggagaagac
     3121 gcactcgatc cccaaggcac tggtcgagat cggcggaaag ccgatcctct ggcacgtgat
     3181 ccggatctac gccgcgcagg gacaccgtcg cttcctgctg ttggccggat acctgcgcga
     3241 gcaggtcgag gcgttcgtcg cgagggcgca gtgggaggag ccgatcgcgg tcgagtgcct
     3301 cgacaccggg ctcgacacca acacgggcgg gcgcgtggcc agggcggccg accgcctgcg
     3361 cgcgggcacc ttctgcctca cctatgccga tggcgtcgcc gacatcgacc tcgcctcgca
     3421 agccgcgttc caccggcgcc aggaggccct cgtgacgatg accgtggtcc ggccctacag
     3481 ccagtggggg atcgcgctga tcgacgagca ggagcggatc accggctttc gcgagaagcc
     3541 caggctcgac tactggatca acgggggctt cttcgtctgc gagccggcgt tcctcgatca
     3601 cgtgggaccc gactcggtgc tcgagcgcgt gccgctcgag ctcgcggcag cgtcggggag
     3661 gctcgcggcc taccgccacg agggcttctg ggactgcatg gacacctaca aggacgcggt
     3721 cacgctcaac gatctatggc agtccgggcg cgcgccgtgg cgggtgtggg agcgggcggg
     3781 ggtcgaggcc ggttgagcgt cgcgctggtc accggcgcgc gcggcttcgc cggcgcctgg
     3841 ctcgcccgag ccctgctcga gcgcggcgat cgcgttgtct cgctcgacaa gggccgcccg
     3901 gagcgccccg cgtcgaccct gtcgctgctc gggatcgaag ccgacgtgat cgacgtcgtc
     3961 ggcgacctct gcgacggcga tctcgtgcgc tcggtgttga gcgagaaccg ggtgaccgat
     4021 gtctaccacc tggcggcgga gccgatcgtc ggcgcgttcg ccgcctcgcc gttgcgcggc
     4081 ttcgagacca acgtccgcgg cacctggacg ttgctcgacg cgtgtctggc cgaggcggcc
     4141 gagcgcgtcg tggtcgcgtc ctcggacaag gcctacggcg cccacgagac gctgccctac
     4201 cgcgaggact tcgctctcaa cgcgaccgcg ccctacgagg cgtccaaggc ggcggcggac
     4261 gtgatcgcac gcagctactg gcacagctac gggctgccgg ttgcagtgac gcgcttcgcc
     4321 aatatctacg gaggcggcga cctgaacttc tcacgcctgg tccccgaggc ggtgtgcgcg
     4381 gcgctggacg gtcgcccacc ggtgctgcgc tccgacggct cgccggagcg cgacttcctg
     4441 tacgtcgagg acgcggcagc tgcgtacctg gcgatcgcgg atgagctcga ccgcgacgaa
     4501 gtgcgcggcg aggctttcaa cgccggcgac gggcgaccgt accgcgtcgg cgaggtggtc
     4561 gagacgatcg cccgcctctc gggcacgggc gtcgagcccg aaatccgggg ggagggaaac
     4621 cccgcggggg agatcgaccg ccagttcgtc gacccgagca agctgcgcac ccgctgcgga
     4681 tgggagccgt ccgtgacgct cgaggccggc ctcgagcgca cgatcgactg gtaccgggag
     4741 caccccggcg tgcgccccgc cggctgaacg gctccgtgca gccccgataa gggccgtagc
     4801 gccgagagga tcgggcgccg ctcccgcgta atcgctctcg gaccgacaga gaggaccggc
     4861 gcgatgcgcg acgagcaccg cacgggggga aacctggtcc gggccgcgcc gatcgcgctg
     4921 ctcggcacga tcgtggccct ggccgcgtgg ccgcagacgg ctcccgcggc ggtgacccac
     4981 gtggtccagc ccggcgaaag cctctggggg atcgcggtgg ccaacaacct tacgacccga
     5041 accgtggccg tcttcaacgg gctgcccgag gacgcgctgc tgatcgccgg cgagtcgatc
     5101 gaggtcccca ccgtcgacga gggggcggcg gcgctcgcga gcgccggcac ggcctccacc
     5161 gcagtatccg gtgccggcac cgaccacctg gttcagccgg gcgagagcct gtggtcgatc
     5221 gcgtcggcga acggcctcac cgtgcaggag gtcgcctcgt tcaatggcct ggccgaggat
     5281 gcgctgctga tcgccggtga gacgatccag gtgcccgggg tgtcgagtgg cacttcggcg
     5341 ggaacgacgg tcgacccgag caccggcctg gcctacctgt ggtcgcccga gggcagcgtc
     5401 cccctcgacc ccgccgcggc cgcatcgttc gagcagatgc gccaggcggc tctgagccag
     5461 ttcgggatcg acctctaccc ggccggcctg ctctccggat atcgcaccta cgagcagcag
     5521 gcctacctct acgacctcta cctctccggc cagggggccc cggcgaaccc gccgggcacc
     5581 tcctcgcacg agtacggcgc cgcggtcgac ctggcggacc cggcgatgcg caccgtggtc
     5641 gaccagatcg ggggcgtcta cggctgggcg gcgacgatcc cgtcggagtg gtggcacgtc
     5701 gcgtatgtcg gtggctagtc tgccggcatg aacgacgccc cgaccgacgt ccaggacgac
     5761 gcccgcaagc aggcgatcag gcggctgaag gccaagcgcg acttcaagtc cttcctcggc
     5821 acggcggcca tcgcgatcgt cgtcgtcatc gtggtctggg cgctcagcga cagcgactac
     5881 ttctggccga tctgggtcat tctcggcctc gggatcgccc tcttcttcag cggcctgcag
     5941 gcctacggtc cgcaccgcgg cccgatcacc gaagccgaga tccagcggga gatcgacaag
     6001 ggcacctagc tcgcggcggc gccgcgctag tccgaccgcc cggcggcttc cagggcctcc
     6061 gctcgtgtgg agtaggaacg tcgctcagcg agccgaccgt cccggatggt ccagacctcg
     6121 accagctgga cgctgatctc ctggcgcccg cccttcggca cggcgtgctg ggtgctcatg
     6181 acgacaaccc gctgtccggc gtccactacc tcgtcgacct ccagtcgaac gctctcgaac
     6241 tgcgcgaaca ggtcctcgtt gtagctccgg acggcatccc tgccgcgata gacgcctgcc
     6301 gcgggcgcgt tcgcgtaggt gtgaaactcg atctcggggt ggaggccgtc agacgcggcc
     6361 tcgaggtcgt tgcgggcgac ggccgcatag acctggcgaa tgagctcgat gttctcctgc
     6421 gacatcgccc gctcagtatc ccgcgtgcgc tgtcaatcct tctggaagct gtagcgacca
     6481 gccgagcctc tcgcatcggg cgtgccgaag caaacgaggc gctcggaagg cgctggcgtc
     6541 tactccctca ggccggcggc tccgagggcg tcgtcccgcc tgatgtagaa actcaccttc
     6601 gccaccctgc ctccgcgcat cacaaagagg aaccagaaat ccccctcgac cggggcgccg
     6661 ctggcttccc cgcgcgccgt ctgacgaacg cgcaccaaga ccctttcccc ggcatcgatg
     6721 aactcctccg gggtcaccga ccactcgctg aaatcttccg tccagtcagc ggtggcgctg
     6781 agaaagccct ccttgccgtg gtaggtcatc ccatccgggt cggctcgata ggtgaccagg
     6841 tcctcggcca tcaaatcgag catccgggtc aaatctccct cttggaaggc gtcgaagccc
     6901 tcgcgcacga gctcgacgtt ctcctgcgac atcgcctacc ccgacagccc ggcggcttcg
     6961 agggcttgcc gacgatcaag aaatgcccgt attcgagtga cgcggccatc ctcgaagctc
     7021 caaacgtgaa agagcgggac ccgagcgctg gctccgctcc cagcgcctgt tcccagccat
     7081 tgcatcgagg cgagcacccg gtcgccgtta gcgatgaact cagccgccgc gaattccata
     7141 tctggaaaga cctcctgcaa cttcccgaac acggcacgga tgccgtcatg gccgtgatgg
     7201 gtcgaggagt cgggaatctc tggaggttcg gtgacctcaa tattgggatc gcacaactcg
     7261 aaaagcagcg cgagctcccc ggtggcgata gcggagtaca ccgcccggag cctttcgacg
     7321 ttctcctccg acatcgcccg cccagtatct cgcgcccgca gggcgtgagg cgggtcctgg
     7381 tcggggctca tcaagtctcg atcggcgccg gtcccagcag gctcggtctc gggcgcgagc
     7441 agagagacct cggcggtgtt cgattccgga tcgatctggc tggaaaggcg gcgcacccgt
     7501 cggccgatcg cctgctgtac ctcggccgag tcctggagag gcgttaaccg tcgtcatggc
     7561 ggggaaggcg gagactctga ccggaacggt gtggtggggc ggggccgcat cccggttatc
     7621 gagcgaccgg cgccgcccac tctcggc
//
LOCUS       DASRIG010000488         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3893791, whole genome shotgun sequence.
ACCESSION   DASRIG010000488 DASRIG010000000
VERSION     DASRIG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893791"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFB52_13055"
     CDS             complement(<1..578)
                     /locus_tag="VFB52_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07310.1"
                     /translation="MSSEKTKPGRPYELKERARRQEETRRRIAAATAELHEEVGPAKT
                     TIAEIARRAGVQRPTVYNNFPSERVLFAACQAHFFGQHPLPDPSAALALAEPSKRAAA
                     TLELFYGWYRETAAMTGNIQRDRRLLPELDGLLADGADQLLDQMAAELARPFRLRGKR
                     AQRGRAALRLALDFATWERLSGEGLSDREAAEL"
     gene            610..1077
                     /locus_tag="VFB52_13060"
     CDS             610..1077
                     /locus_tag="VFB52_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO07311.1"
                     /translation="MDSDRADFPNLGNRFVRRSEQTGGEFALVEHTLAPHSLAAPTHT
                     HRNEDEYSFVLSGRVGGHVGDEFLDLGPGELLVKPRGVPHAFWNAGAEEARVLELITP
                     GGFERYFAELAPLLNAEGGPDLPAIAATCERYELAMDFESIGPLAERFGLQAF"
     gene            complement(1106..1711)
                     /locus_tag="VFB52_13065"
     CDS             complement(1106..1711)
                     /locus_tag="VFB52_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07312.1"
                     /translation="MLLLAFASPAAQADPVFEFSNSSGDLTGIGGSSEKITTEAGTIV
                     CNKIEIAAPYSASSQSTLSFVTVHSECKAFGFLSATVDSEICAYVFDPYEKVGEGNYR
                     AKFSVDCGAGESIKVVASTCSMEIQDVAGNQGLSYVALTNSENKILMDPNVSPIVYQV
                     TNDGFLCPFNGTGKKTDGTYTSTAAVKLTAAGGATAKVAGS"
     gene            complement(1946..>2535)
                     /locus_tag="VFB52_13070"
     CDS             complement(1946..>2535)
                     /locus_tag="VFB52_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07313.1"
                     /translation="TVYAGPLATMPAQELGRIDPGEPRRFEFVATLPEAGPAVDQNAL
                     QGAAMNVAYIWTASEATDDPERRPPGGGGDAGGTPAADELDLAVTKVRHRIRRGRLVV
                     WLSCDHPCAVAVRGRLRARDEDDRRRAKLRSRADHTFEAGAQKLTVRLPRGMRRWLRA
                     DPSHERVRARATFVAWDQAGDRDTVKRALRLRSAR"
BASE COUNT          367 a          874 c          859 g          435 t
ORIGIN      
        1 agctccgccg cttcgcggtc gctgaggccc tcgccgctga gccgctccca ggtcgcgaag
       61 tcgagcgcca ggcgcagcgc tgcccgcccc cgctgcgcgc gcttgccgcg caggcggaac
      121 ggccgcgcca gctcagcggc catctggtcg agcagctggt cggcgccgtc ggcgagcaga
      181 ccgtcgagtt cgggcagcag gcgccggtca cgctggatgt tgccggtcat cgccgccgtc
      241 tcccggtacc agccgtagaa cagctcgagg gtcgcggccg cccgcttcga gggctcggcg
      301 agcgccagcg ccgcgctcgg gtcgggcaac gggtgctggc cgaagaagtg cgcctggcag
      361 gcggcgaaga gcacacgctc gctggggaag ttgttgtaga cggtcggccg ctggaccccg
      421 gcccggcgcg cgatctcggc gatcgtcgtc ttcgccgggc cgacttcctc gtgcagctcc
      481 gcggtcgccg cggcgatccg ccgacgggtc tcctcctggc gccgggcgcg ctccttcagc
      541 tcgtaggggc ggccgggttt cgtcttttca cttgacatgg cgtatagcga aatgtactct
      601 cgccgtccga tggattccga ccgcgccgac ttccccaacc tcggcaaccg cttcgtccgc
      661 cgcagcgagc agaccggcgg cgagttcgcc ctggtcgaac acaccctggc cccgcactcg
      721 ctcgccgccc ccacccacac gcaccgcaac gaggacgagt actcgttcgt gctcagcggc
      781 cgtgtcggcg gccacgtcgg cgacgagttc ctcgacctcg gcccgggcga gctgctggtc
      841 aaaccgcgcg gcgtccccca cgccttctgg aacgccggcg ccgaggaggc gcgggtgctc
      901 gagctgatca cacccggcgg cttcgagcgc tacttcgccg agctggcgcc gctcctgaac
      961 gccgagggcg gcccggacct gcccgccatc gccgccacct gcgagcgtta cgagctggcg
     1021 atggacttcg agtcgatcgg gccgctggcc gagcgcttcg ggctgcaggc gttctagcgc
     1081 ggagaccggg ctagaaccga tcgactcatg acccggccac tttggccgtc gcgccaccgg
     1141 cggcggtcag tttcaccgcg gcggtcgacg tgtaggtgcc atcggttttt ttgccggtcc
     1201 cgttgaacgg acagagaaac ccgtcgttgg tgacctgata gacgatcggc gagacgttcg
     1261 ggtccatgag gattttgttt tcagagttgg tcagcgcgac gtaggacaga ccctgattgc
     1321 cggcaacatc ctggatttcc atcgagcagg tgcttgccac gacttttatc gattcgccgg
     1381 caccgcaatc gacgctgaat ttcgcccggt agttcccttc accgactttt tcataggggt
     1441 cgaacacata tgcacatatt tcagagtcga ccgtcgcgga gagaaatcca aatgctttgc
     1501 attcactgtg gacggtgaca aagctcagcg ttgactggct ggaggcgctg tagggagcgg
     1561 cgatttcgat tttgttgcag acgatcgtcc cggcttcagt ggtgattttt tcgctggatc
     1621 caccgatgcc tgtgaggtcg ccagacgagt tcgagaattc gaacacggga tcggcctgag
     1681 cggcggggga agcaaacgcg agcagcagca tcgcgacaat cagtgcaccc gttattttcg
     1741 cccttccaat catcagcgac cccctgtcgt atgccggagt ccatcttgta acgaggggta
     1801 gatcgggcat caagagttcc cgtgcatttg ccactcggcg cggcaagcga ctcgggtgag
     1861 acgagcatct cccctgcatc tccggtgtcg ccgatttgcg cgtgcgcaga tccgtacgac
     1921 gcagcattgg gcgcgccggt gaagttcagc gcgctgagcg caggcgcagc gcccgcttca
     1981 ccgtgtcgcg atcgccggcc tggtcccagg cgacaaaggt cgcgcgggct cgcacccgct
     2041 cgtggctcgg gtccgcccgc agccagcggc gcatcccgcg cggcagcctc acggtcagct
     2101 tctgggcgcc ggcctcgaag gtgtggtcgg cgcgggagcg cagcttggcg cggcgccggt
     2161 cgtcctcgtc gcgggcgcgc aggcggccgc ggaccgcgac cgcgcacggg tggtcgcaac
     2221 tgagccagac gacgaggcgg ccgcggcgaa tccggtggcg gaccttggtc accgcgaggt
     2281 cgagctcgtc ggccgccggg gtgccgccgg catctccccc gccgccgggc ggtcggcgct
     2341 cggggtcgtc cgtggcttcg ctcgcggtcc atatatacgc gacgttcatc gcggccccct
     2401 gcagcgcgtt ctggtcgacg gccggccccg cttccggcag ggtcgcgacg aactcgaagc
     2461 ggcgcggctc gccgggatcg atccgcccca gctcctgcgc cggcatcgtc gccagcggac
     2521 ccgcgtaaac cgtgg
//
LOCUS       DASRIG010000489         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3616395, whole genome shotgun sequence.
ACCESSION   DASRIG010000489 DASRIG010000000
VERSION     DASRIG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616395"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFB52_13075"
     CDS             complement(<1..1136)
                     /locus_tag="VFB52_13075"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO07314.1"
                     /translation="MKVPTGEAIGARLGAAAAWAARNAKVVLVVALVVAIGAAVGATQ
                     ISTDAGVSTLVDTDTESYEATKDVEQSFGQEPVVVLARGDLPELVLTNNIFRLLRLEG
                     CLAAKVPKGGKPLPGPCTELAEMEPVEFVAGPATFLNEAVVQIEKQLRNLAARVPAEK
                     FQEFLLTVATRYGITSVPTLDNEEFVATVVFDLARPRGTPKARLAYLFPNSHTAQIIV
                     RLKPGLSDSERHRALTLIEEAVEETAPRKACAENGKAAPCFELDKGSYVISGAPVVVD
                     GVARALKDALLILFGVAVVVMAVTLLLVFRSRLRLLPLAIALAAAALTFGLLALVGGA
                     LTMASIAVLPILIGLAVDYAIQFQARFDEATEEGLSGGEAARAAA"
     gene            complement(1137..2132)
                     /locus_tag="VFB52_13080"
     CDS             complement(1137..2132)
                     /locus_tag="VFB52_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZO07315.1"
                     /translation="MSVEDENRREEEELQDEAVMGGEEPPEIGERAVPARADNDSGPY
                     VSDDGAVMGRDFVIPGVAPDEHDPYRFHTGRKRDHGGEDAIEIIDLVKQFGRARILNG
                     LNLGLPEDQVSMVLGPSGTGKSVLIKHIVGLLYPDSGDVLVHGESIPNMTDDELFEVR
                     KKFGLLFQDGALFGSMNIYDNVAFPLRQHTDKGEEEIGEIVNRRLKEVGLAEATYKMP
                     NELSGGMRKRAGFARALVLEPAIVMFDEPDSGLDPVRTALLCELIREVHAENGGCYLV
                     ISHDLGTARRIADFIAVLWSGKIVESGPKEEVFDSSNPFVNQFLHADITGPLSMD"
     gene            complement(2134..>2719)
                     /locus_tag="VFB52_13085"
     CDS             complement(2134..>2719)
                     /locus_tag="VFB52_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07316.1"
                     /translation="AYFNRSVGAPAYAGVFSAWCDLREIMPYAFGYMLAAKVGTGIVA
                     ELGAMRISDEIDALEVMGVPPVTFLCATRLLAAWITFPFMYLVGIGIMYLASYLAVVE
                     QIGDVSSGGYFLIFWMFQNPPDLIFSLIKGMVMATAIVLVGCYYGYTASGGPVGVGTA
                     TAKSMVLNIVLVHIIGMLGTLVFWGANPRAPIGG"
BASE COUNT          430 a          877 c          963 g          449 t
ORIGIN      
        1 gcggcggcgc gagccgcctc tcccccgctc agcccttcct cggtggcctc gtcgaacctc
       61 gcttggaact ggatcgcgta gtcgacggcg aggccgatca ggatcgggag gaccgcgatc
      121 gaggccatgg tcagggcgcc gccgacaagg gcgaggagac cgaaggtcag ggcggcggcg
      181 gcgagcgcga tcgccagcgg cagcagacgc aggcgcgagc ggaagacgag caacagggtc
      241 acggccatca ccaccacggc gacgccgaag aggatcagca gggcgtcctt cagcgcccgc
      301 gcgaccccgt cgacgacgac cggcgcgccg gagatcacgt agctgccttt gtccagctcg
      361 aagcagggcg cggctttgcc gttttcggcg caggctttgc gcggggccgt ttcctcgacc
      421 gcttcctcga tcagggtcag cgcccggtgg cgttcggagt cgctgaggcc gggcttgagg
      481 cgaacgatga tctgggccgt gtggctgttc gggaagaggt aggcgagccg cgccttcggg
      541 gtgccgcgcg gccgcgccag gtcgaagacg acggtggcga cgaattcttc gttgtcgagg
      601 gtggggacgc tggtgatccc gtagcgggtc gcgaccgtga gcaggaattc ctggaatttc
      661 tcggccggca cccgcgcggc gaggttgcgc agctgcttct cgatctggac caccgcctcg
      721 ttgaggaagg tggccgggcc ggcgacgaac tcgaccggtt ccatctcggc gagctcggtg
      781 cagggacccg gcagcggttt gccgcctttc gggaccttgg cggcgaggca gccctcgagc
      841 cgcagcagac ggaagatgtt gttggtgagg accagttccg gcaggtcgcc gcgggccagc
      901 acgaccaccg gttcctggcc gaaggactgt tcgacgtcct tggtcgcttc gtaggactcg
      961 gtgtcggtgt cgaccagggt cgagacgccg gcgtcggtcg agatctgggt cgccccgacc
     1021 gcagcaccga tcgcaacgac cagcgcgaca acgaggacga ccttcgcgtt acgcgccgcc
     1081 caggcggcgg ccgctcctag gcgggcgccg atcgcctcac cagtcgggac cttcactcag
     1141 tccatcgaca gggggccggt gatgtccgcg tggaggaact ggttgacgaa cgggttgctg
     1201 gagtcgaaga cttcctcttt gggccccgac tcgacgatct tgccgctcca cagcaccgcg
     1261 atgaagtcgg cgatccggcg ggcggtgccg aggtcgtggc tgatcaccag gtagcagcca
     1321 ccgttctcgg cgtggacctc gcggatcagc tcgcagagca gcgcggtgcg caccgggtcg
     1381 agcccggagt cgggctcgtc gaacatcacg atcgccggtt cgagcaccag cgcccgggcg
     1441 aagccggcgc gcttgcgcat cccgccggag agctcgttcg gcatcttgta ggtcgcctcg
     1501 gcgaggccga cctctttgag ccggcggttg acgatctcgc cgatctcttc ctcgcctttg
     1561 tcggtgtgct ggcgcagcgg gaaggcgacg ttgtcgtaga tgttcatcga gccgaagagg
     1621 gcgccgtcct ggaacagcag gccgaacttt ttgcgcacct cgaagagttc gtcgtcggtc
     1681 atgttcggga tcgactcgcc gtgcacgagc acgtcgccgg agtccgggta gagcagcccg
     1741 acgatgtgct tgatcagcac gctcttgccg gttcccgagg gaccgagcac catcgagacc
     1801 tggtcctcgg gcaggccgag gttgaggccg ttgaggatgc gggcgcggcc gaactgtttg
     1861 accaggtcga tgatctcgat cgcgtcctcg ccgccgtggt cgcgcttgcg gccggtgtgg
     1921 aagcggtagg ggtcgtgctc gtcgggggcg acgccgggga tcacgaagtc gcggcccatc
     1981 acggcgccgt cgtcacttac gtagggcccg ctgtcgttgt cggcgcgcgc cggcacggcg
     2041 cgctcgccga tctccggcgg ctcttcgccg cccatcacgg cctcgtcctg cagctcttct
     2101 tcttccctgc ggttctcgtc ctcgacagac acgtcagcct cctattggcg cgcgtgggtt
     2161 ggcaccccag aagaccaggg tcccaagcat cccgatgatg tggactaaga cgatgttgag
     2221 gaccatcgac ttggcggtcg ccgtccccac tcccaccggc ccgccgctgg cggtgtagcc
     2281 gtagtagcag ccgaccagca cgatggccgt agccatcacc atgcccttga tcaggctgaa
     2341 tatgaggtcg ggcgggttct ggaacatcca gaagatcagg aagtagccgc ccgaagagac
     2401 gtcgccgatc tgctcgacca cggccagata gctggccagg tacatgatcc cgatcccgac
     2461 caggtacatg aacgggaagg tgatccaggc ggcgagcagg cgggtggcgc agaggaaggt
     2521 gaccggcggc acgcccatta cctcgagcgc gtcgatctcg tcggagatcc gcatcgcccc
     2581 gagctcggcg acgatcccgg tcccgacctt ggccgcgagc atgtagccga aggcgtaggg
     2641 catgatctcg cgcaggtcgc accacgcgct gaagacgccc gcgtatgccg gcgcgccgac
     2701 ggagcggttg aagtaggcg
//
LOCUS       DASRIG010000490         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1533329, whole genome shotgun sequence.
ACCESSION   DASRIG010000490 DASRIG010000000
VERSION     DASRIG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533329"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFB52_13090"
     CDS             <1..332
                     /locus_tag="VFB52_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07317.1"
                     /translation="GTVTQVAKLKVTKPKLKLGAVKLSPAKGTATLEVKVPGAGSVTV
                     SGKDVVKTKRSAKGAKKLKLTIRPRGNAKTALLQTGSAKVKAKLTFKPKSGSSVAKTK
                     TITLQLG"
     gene            350..423
                     /locus_tag="VFB52_13095"
     tRNA            350..423
                     /locus_tag="VFB52_13095"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Trp,seq:cca)
     gene            558..1190
                     /locus_tag="VFB52_13100"
     CDS             558..1190
                     /locus_tag="VFB52_13100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07318.1"
                     /translation="MVNNDMEASMTTQSRLDLTEYRPFPNEQGRNTRQAQLEIPVLVR
                     ALGLPTDVRILEIGCGRGVALPVLDRLCTPRRLVGLDIDEELLVEAADNLREHGTEAE
                     LCCGDVRRMPFADEAFDVIIDFGTLYHIAGSQFALDEITRVLAPAGTFAYETKASQFL
                     SHPVRSRGRRLPALTTHGLRHRRWAMLWASRTKALSPEGEKPRASVAAGA"
     gene            1370..2017
                     /locus_tag="VFB52_13105"
     CDS             1370..2017
                     /locus_tag="VFB52_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HZO07319.1"
                     /translation="MLAGIALVLALGGTAVAATGLIRAADIAPGAVTGQAIRNGGVEP
                     RDLSAQTRALLQAGRRGPTGAPGTNGANGPNGANGADGRNGTDGTDGIDGTDGIDGTI
                     APLSATSGLTTLPTAAPMTTVVSLAVPAGNYVVLAKTQLSHTGAGDTVECVLKAGATT
                     LDQIAMKTLPALAAIPASLQAVTTVSSPTQLAVECKVQVANGAANFSSLIAIPTG"
     gene            complement(2045..>3440)
                     /locus_tag="VFB52_13110"
     CDS             complement(2045..>3440)
                     /locus_tag="VFB52_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZO07320.1"
                     /translation="RVLVVNDQVEMLRLVDRALGDPYRCEFAASVAQAHHKLASANFA
                     LALCDIQMVDGSGFDLARGITEEHPKTAVVLITDRDDPELARKAFDFGEHGIHGYLVK
                     PFWPGQLLITVMNALRRRELEGAELSHRENLEDRRQTIIDRAPTPIYVKDGDFRYVFA
                     NAAADELAGQGDGGLIGKTDEAIMPPRTLAQVRADDRRILAEGTAHQAEEALSVGGVE
                     RIFKTIKFPLLDQQGRPTAVCGISADITSQIEALRLRDELADSQQQAIEELQLSRQET
                     VERLTRAIGLHDHSTGEHVNRMAEVAGLLGSMLGLPPTRIQLLRLAAPMHDVGKIATS
                     SAILRKNGPLSPAEREEMETHTTVGHEILADSESELLRLAATIALTHHERYDGGGYPH
                     GLAGEEIPLEGRITAVADVFDALLSERAYRPAMTVDEALEIIRAGRGSHFDPRIVDLL
                     LDHLDWALAVRTAA"
BASE COUNT          559 a         1120 c         1201 g          560 t
ORIGIN      
        1 ggggcaccgt gacccaggtt gcgaagctga aggtgacgaa gccgaagctg aagctgggcg
       61 ccgtgaaact gagcccggcc aaaggcaccg cgaccctcga ggtcaaggtc ccgggcgctg
      121 gcagcgtcac cgtctccggc aaagacgtgg tcaagacgaa gcgcagcgcc aagggcgcga
      181 agaagctgaa gctgacgatc aggccgcgcg gcaacgccaa gacggcgctg ctgcagacgg
      241 gcagcgcgaa agtcaaagcg aagctgacct tcaagccgaa gtcgggcagc tcggtcgcga
      301 agaccaagac gatcactctg cagctcggct agctaggatc gacccctctg gggcagtggc
      361 ccaactggct aaggcaccgg tctccaaaac cggcgattcc aggttcgagt cctggctgcc
      421 ccgtacctcc ctctgcggct ccaagagtcg ctctccgctc tagtaagccg aagacacgcc
      481 gtcagacgga gtgcgttcgg cgctgttccg attctcctgt tgaccgtcgg tcgacaagtg
      541 atatacgttg ttgaccgatg gtcaacaacg atatggaggc atcgatgacg actcagagca
      601 ggctcgattt gaccgagtac cgtcccttcc ccaacgagca ggggcggaac actcgacagg
      661 ctcagctgga gatccccgtt ctggtgcggg ccttggggtt gccgaccgac gtgcggattc
      721 tcgagatcgg ctgcggacgc ggggtggcgt tacccgttct tgaccgactg tgtacgcctc
      781 gacggctggt gggtctcgat atcgacgagg agctgttggt ggaggctgcc gacaatctcc
      841 gggagcatgg gaccgaggcg gagctgtgtt gcggtgatgt gcgccggatg ccgttcgccg
      901 atgaagcgtt cgatgtgatc atcgatttcg gcacgctgta ccacatcgcc ggatctcaat
      961 ttgcgcttga cgagattacg cgcgttctcg cgccggcggg cacgtttgcc tacgagacga
     1021 aggcctcgca gtttctttcg catcccgtcc gttcccgagg tcgacgtcta ccggcgctga
     1081 cgacccatgg cctgcggcac cggcgctggg cgatgctctg ggcttctcgg accaaggcgc
     1141 tttcacctga aggggaaaag cccagggcat cggtagcggc cggggcctga gcagcgcgaa
     1201 atagctcacg ctccactcgg tccctgtcga tgggtcctgt ggcggcagca gctgctaccg
     1261 ccccaggtgc cgggtaaggc gcgcagtcag ccaagggagc acccagatga agcgtgtagc
     1321 cagaagccct cgcaagaagg agcggcgccg ctggcctacg ccgtcgctgt tgcttgccgg
     1381 cattgccctc gtcctcgcgc tcggcggcac cgcggtcgcc gcgaccggcc tgatccgggc
     1441 cgcggacatc gctcccggcg cggtcacggg gcaggcgatc cgcaacggcg gggtcgagcc
     1501 cagggatctg agcgcgcaga cgcgagcgct gctgcaggcc gggcgaaggg gccccaccgg
     1561 cgcacctggc accaacggcg ccaacggccc gaatggcgcc aacggcgccg acggcaggaa
     1621 cggaaccgac gggacggacg gtatcgacgg cactgacggg atcgatggga ccattgcccc
     1681 gctttccgcc accagcgggc tcacgacgct tcccaccgcc gccccgatga cgaccgtcgt
     1741 cagcctcgcg gttccggccg gcaactacgt ggtcctggcg aagacccagc tgtcccacac
     1801 gggggccggt gacaccgtcg aatgcgtgct gaaggcgggc gcgaccacgc tcgaccagat
     1861 cgcgatgaag acgctgcccg cgcttgcggc gatccccgct tccctgcagg cggtgaccac
     1921 ggtgagctcg cccacccagc ttgccgtcga atgcaaagtc caggtggcaa acggcgcggc
     1981 caacttcagc agtctgatcg cgatccccac cggctgagcg gagaggcggc gcctggcgac
     2041 caggtcaggc ggcggttctg accgcgagcg cccagtcgag gtgatcgagg aggaggtcga
     2101 cgatccgggg atcgaagtgg gatccccggc ccgccctgat gatctccagc gcttcgtcga
     2161 ccgtcattgc cggccggtag gcgcgctcgc tgagcagggc gtcgaagacg tcggccaccg
     2221 cggtgatccg tccctccagc gggatctcct cgccggccag gccgtgggga tagccgccgc
     2281 cgtcgtagcg ctcgtggtgg gtcaaggcga tcgtcgccgc gaggcgcagg agctcgctct
     2341 cggaatcggc gaggatctcg tgcccgaccg tggtgtgggt ctccatctcc tcccgctccg
     2401 cgggtgagag agggccgttc ttgcgcagga tcgcgctcga ggtggcgatc ttgccgacgt
     2461 cgtgcatcgg ggccgccagg cgcaggagct ggatccgcgt cggcgggagc ccgagcatcg
     2521 agccgagcag accggcgacc tcggccatgc ggttgacgtg ctctccggtg gagtggtcgt
     2581 gcaggccgat tgccctggtc aatcgctcga cggtctcctg gcgggagagc tggagttcct
     2641 cgatcgcttg ctgctgggag tcggccagct cgtcgcgcag cctcagcgcc tcgatctggc
     2701 tggtgatgtc ggcggagatc ccacagaccg cggtgggccg cccctgctgg tcgaggaggg
     2761 ggaacttgat cgtcttgaag atccgctcga cgccgccgac cgagagggct tcctcggctt
     2821 ggtgggccgt gccctccgcg aggatccggc gatcgtcggc gcggacctgc gcaagcgtcc
     2881 gcgggggcat gatcgcctcg tcggtcttgc cgatcagccc accgtccccc tgtcccgcca
     2941 gctcgtctgc cgccgcgttg gcgaagacgt agcggaagtc gccgtccttg acgtagatcg
     3001 gcgtcggcgc ccggtcgatg atcgtctggc gcctgtcttc gaggttttcc cggtgggaga
     3061 gctccgcgcc ttcgagctcg cgccggcgga gggcgttcat caccgtgatc agcagctggc
     3121 cgggccagaa cggcttgacc aggtagccgt ggatgccgtg ctcgccgaag tcgaacgcct
     3181 tcctcgcgag ctcggggtcg tcccggtcgg tgatcaggac gaccgcggtc ttcgggtgct
     3241 cctcggttat cccgcgggcc agatcgaatc ccgacccgtc gaccatctgg atgtcgcaga
     3301 gggcgagcgc gaaattggcg gaggcgagct tgtggtgggc ctgggcgacg gaggccgcga
     3361 actcgcagcg atagggatct cccaaggcgc ggtcgaccag gcgcagcatc tcgacctgat
     3421 cgttgacgac gaggacgcga
//
LOCUS       DASRIG010000491         7465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1533342, whole genome shotgun sequence.
ACCESSION   DASRIG010000491 DASRIG010000000
VERSION     DASRIG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533342"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFB52_13115"
     CDS             complement(<1..362)
                     /locus_tag="VFB52_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07321.1"
                     /translation="MRARFWIGLAAVCLIGVGAIAGALFIHADDTNDLAQIEREEAVR
                     AAQQTEAVASFSVGQLSTSAAFIQAQGNLNAHEFEVLGRSLLSEGVLDSTAYLPRVRG
                     PERAAYERSSGHEILERPP"
     gene            complement(437..1081)
                     /locus_tag="VFB52_13120"
     CDS             complement(437..1081)
                     /locus_tag="VFB52_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZO07322.1"
                     /translation="MSAAQARAARKALAGSDPVMAALIERIGKIDLETRLARRREEAP
                     PDAYGALLRAIVGQQLSTKAARTIYLRVVDLFGGTTPSPEQLLAASVEDLRAAGLSGR
                     KVEYVQDLARHVLSGELELDRLDQLEDEQVIEEIVAVRGLGRWTAEMFLLFHLQRPDV
                     LSGGDLGIRKAIQVEYGLEQMPTPQETVAIGERFSPHRSLASLYLWESLAAVPD"
     gene            complement(1078..1884)
                     /locus_tag="VFB52_13125"
     CDS             complement(1078..1884)
                     /locus_tag="VFB52_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO07323.1"
                     /translation="MNEQLASGKLLLDRPAEAVARLRIANPERRNALDHEILDALAEV
                     MPRLDQGIETRCVLITGEPPVFSAGYDIAGIPSETFERDAEALVAHPFHAAMEAISRH
                     PWPTVAAINGHCLGGGLELAITCDLRICASGALLGMPPAKLGLIYGHTGLRKFLDTIG
                     LARTKELFLTGRNLEAARADEIGLVNEVVSDDRLGHEAVQLAAAIAANAPLSMRGNKS
                     AIDTLNASPVLSEQQEAELVALRESCFASADFREGIRAFAEKRRPEWTGE"
     gene            complement(1888..2100)
                     /locus_tag="VFB52_13130"
     CDS             complement(1888..2100)
                     /locus_tag="VFB52_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HZO07324.1"
                     /translation="MADIEAHITGNVWKIEVAIGDEVSDGDTVAILESMKMEIPVESE
                     DDGKVKEIRCEEGQAVSEGDVLIVLE"
     gene            2166..2882
                     /locus_tag="VFB52_13135"
     CDS             2166..2882
                     /locus_tag="VFB52_13135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO07325.1"
                     /translation="MAKNENSEGDLAVIVAARNEAERIAATVGALRATFPGAAIWVAD
                     DASSDGTGERALAAGAQVVSRGKPHGKGANVSAAAEAALSAPEPPELVLLCDGDLGAS
                     AGRLGPLVEGVRAGECDLAVAAFARRVGGGFGVALGFAHWAIRRLSGQETTAPISGQR
                     ALRVDALRATLPFAAGFGMEIGMTADAVRAGYTLREYEIDLEHRATGRSLAGFRHRGV
                     QLRDFLRVYLSRMGGRGSGR"
     gene            2879..4324
                     /gene="glpK"
                     /locus_tag="VFB52_13140"
     CDS             2879..4324
                     /gene="glpK"
                     /locus_tag="VFB52_13140"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZO07326.1"
                     /translation="MILAIDQGTTGTTCLVFDREGRLVGRAYSEFEQFFPRPGWVEHD
                     ANEIWEVTRRVAAEAIAAAGIEGRELDSIGITNQRETVVAWDPASGEPVHRALVWQDR
                     RTAERCDELRAAGHEALVRERTGLVVDAYFSGTKIEWLLANAEGAERAVFGTIDSWLL
                     YKLTGRHVTDYSNAARTMLFDIRKLAWDAELCALLGVDPARLPEPLPSAGSFGTTTEF
                     GGEVPVCGVAGDQQAALFGQACHAPGDAKNTYGTGSFVLLNTGAEAPPPPDGLLTTVA
                     WGLGDEVAYALEASIFVTGAAVQWLRDGLGIIDAAAETEALAASLDTNDGVYFVPALT
                     GLGSPHWDPYARGTIVGLTRGSGRAHLARAALEGIAYETVDAVRAQEAASGVRLGALK
                     ADGGAVANRWLMQFQADVLGVPVVVPEIAETTALGAAYLAGVAAGLWTREQVGEMWRE
                     AARYEPAMGADERQTLLTGWARALERSRAWA"
     gene            4384..5127
                     /locus_tag="VFB52_13145"
     CDS             4384..5127
                     /locus_tag="VFB52_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZO07327.1"
                     /translation="MAEMKPQVYLDPRPPEYFDQFHRSARKGSVWMYSIARVVLTPLV
                     LLIYRVKAVDKENVPREGALVLAPNHFSQLDHFFVGVWLRRKVRFMAKSQLFGPPVLT
                     FVFKHGGVFPVRRGHRDEEAFKTAYELIDQKEMLLVYAEGGRSRSGEMGEPKPGIGRI
                     ALESGVPIVPVAIYGSAEARRWKRFRFPKITIQFGEPMVFPVGADPSRERQLEVAHAV
                     FADVKRMYEGLATRSEAEAGGRTPDRARA"
     gene            complement(5072..5983)
                     /locus_tag="VFB52_13150"
     CDS             complement(5072..5983)
                     /locus_tag="VFB52_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZO07328.1"
                     /translation="MKVSVEPHPLHEPLAGGTPGATVAVEPMVAGTVDFPMKTMVSPG
                     GRFLTLKLLRALLFKSEPNVVPVPAFLIRHPSAGAILVDTGLHPSIVSDGKENFGGMS
                     NRFGKPALKPGEDVPAQLRDRGLDPNEIPIVVMTHLHIDHTSAISEFPNSTFVVSEPE
                     WVEATTGSGGSLKGYRRQHIDFAFEYRTVSFDRANIDSYASFGRTFDLFGDGSVRLAF
                     TPGHSAGHMSVICRLAKRDFVIGGDAMYMLDQLLGKAALPPRPEDAHNYRRSLQELKL
                     FHSQFPDAVITPGHDPEFYPRLQPRYE"
     gene            6056..6886
                     /locus_tag="VFB52_13155"
     CDS             6056..6886
                     /locus_tag="VFB52_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07329.1"
                     /translation="MFRDGLLAGQVVALSGAGAGLGRESALELLRLGATVVGCGRRSE
                     PLEETAELAADLPGSFEHESLDIREEEPVGRFFDGLLERHGRLDTLVNNAGGQFLSPA
                     EAISPKGFKTVIDLNVLGTWLMTHAAATKAFIPQEGGKVLSVTLSPHNGMPGMVHSGA
                     ARAAVENMMRTLSIEWARFGIKTCSLAAGHFATETLLTKYPPEVVANVARSIPLQRTG
                     RAEEMAWLVAYLASPAGDFFSGTTVTLDGARDNWAGPWPPESIAGEGGAPPAEQRKRD
                     "
     gene            complement(6920..7327)
                     /locus_tag="VFB52_13160"
     CDS             complement(6920..7327)
                     /locus_tag="VFB52_13160"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZO07330.1"
                     /translation="MGAHTFLYRHTGGRIGHSVPGVRGKMLLLDHVGAKSQKLRTSPL
                     LYVDDGPDVVIVASKGGFPKHPAWYHNLMAHPDTVVQIGSERRPVHARVATPEERDRL
                     WEMAVHSYRGYEDYAIRSKGREIPLVVLEPRAA"
BASE COUNT         1038 a         2555 c         2734 g         1138 t
ORIGIN      
        1 ggcggccgtt cgaggatctc gtggccgctg ctgcgttcgt aggcggcgcg ttcgggcccc
       61 cgcacccgcg gcaggtaggc ggtcgagtcg agcaccccct cgctgagcag cgagcggccc
      121 agtacttcga actcgtgcgc gttgagattc ccctgcgcct ggatgaaggc ggccgaggtc
      181 gagagctgcc cgaccgagaa ggaagccacc gcctcggtct gctgtgccgc ccgaaccgcc
      241 tcttcgcgct cgatctgggc gaggtcgttc gtgtcgtcgg cgtggatgaa cagcgcgccg
      301 gcgatcgccc cgaccccgat caggcagaca gcggcgaggc ctatccaaaa acgagcgcgc
      361 atctgtttcc tttcggcggc gaacacgctg ggattgagcc cttgggacgt cccgtgcgca
      421 atctctgaca gcttggtcag tcgggaacgg ctgccaggct ctcccagagg tagagcgagg
      481 cgaggctgcg gtgggggctg aagcgctcgc cgatcgcgac cgtctcctgc ggcgtcggca
      541 tctgctcgag gccgtactcg acctggatcg ctttgcggat cccgaggtcg ccgccggaga
      601 gcacgtcggg gcgctgcagg tggaagagca ggaacatctc cgcggtccag cggccgaggc
      661 cgcgcaccgc gacgatctcc tcgatcacct gctcgtcctc gagctggtcg aggcgatcga
      721 gctcgagctc gccgctgagc acgtggcggg cgaggtcttg gacatactcg accttgcgcc
      781 cggagaggcc ggcggcgcgc aggtcctcga cgctggcggc gagcagctgc tccggcgaag
      841 gggtcgtgcc gccgaacagg tcgaccacgc gcaggtagat cgtgcgcgcc gctttggtcg
      901 agagctgctg gccgacgatc gcccgcagca gcgccccgta ggcgtccggc ggtgcctcct
      961 cgcggcggcg ggccagccgc gtctccaggt cgattttgcc gatccgctcg atcagcgccg
     1021 ccatcaccgg gtccgacccg gcgagcgctt tgcgggcggc gcgcgcctgc gcagcgctca
     1081 ctcgccggtc cactcgggcc ggcgcttctc ggcgaaggcg cggatgccct cgcggaagtc
     1141 ggccgaggcg aagcaggact cgcgcagcgc gaccagctcc gcctcctgct gctcgctgag
     1201 caccggcgac gcgttcagcg tgtcgatcgc gctcttgttg ccgcgcatcg agagcggcgc
     1261 gttggcggcg atcgcggcgg ccagctggac cgcctcgtgg ccgagccggt cgtcgctgac
     1321 cacttcgttg accaggccga tctcgtcggc gcgggcggcc tcgaggttgc ggccggtgag
     1381 gaacagctcc ttggtccgcg ccagcccgat cgtgtcgagg aacttgcgca gcccggtgtg
     1441 gccgtagatc aggccgagtt ttgccggcgg catccccagc agcgccccgg aggcgcagat
     1501 ccgcaggtcg caggtgatcg ccagctcgag gccgccgccg aggcagtggc cgttgatcgc
     1561 cgccacggtc ggccacgggt gccgggagat cgcctccatc gccgcgtgga agggatgggc
     1621 gacgagcgcc tcggcgtcgc gctcgaaggt ctcgctgggg atcccggcga tgtcgtagcc
     1681 ggccgagaag accggcggct cgccggtgat cagcacgcag cgggtctcga tcccctggtc
     1741 gagccgcggc atcacctcgg ccagcgcgtc gaggatctcg tggtcgagcg cgttgcgccg
     1801 ctccgggttg gcgatgcgca gccgcgcgac ggcctcggcg ggccggtcga gcaggagctt
     1861 gccgctggca agctgctcgt tcacgagcta ctccagcacg atgaggacgt cgccttcgct
     1921 cacggcttgg ccctcctcgc agcgaatctc tttcaccttg ccgtcgtctt cggactcgac
     1981 cgggatctcc atcttcatcg actcgaggat cgcaacggtg tcgccgtcgc tgacctcgtc
     2041 gccgatcgcg acctcgattt tccagacgtt gccggtgatg tgcgcctcga tgtctgccat
     2101 gggcggggac aatatccgcg cgcgcggttc ctttcctacg gaatacgtgc aaaaggaacc
     2161 acgcgatggc gaaaaacgag aattcagagg gtgatctggc ggtgatcgtc gccgcccgca
     2221 acgaggcgga gcggatcgcg gccacggtcg gcgccctgcg ggcgaccttc ccgggtgcgg
     2281 cgatctgggt cgccgacgac gcctccagcg acggcaccgg cgagcgggcg ctggcggctg
     2341 gggcccaggt ggtcagccgc ggcaagccgc acggcaaggg cgccaacgta agcgccgccg
     2401 cggaggcggc gctgagcgcg cccgagccgc cggagctggt cctgctctgc gacggcgacc
     2461 tcggcgcctc ggcgggccgg ctcggcccgc tggtcgaggg ggtgcgggcg ggggagtgcg
     2521 acctcgcggt cgccgcgttc gcgcggcggg tcggcggcgg cttcggcgtc gcgctcgggt
     2581 tcgcgcactg ggcgatccgg cggctcagcg ggcaggagac gacggcgccg atctccggcc
     2641 agcgggcgct gcgggtcgac gcgctgcggg cgacgctgcc gttcgcggcc ggcttcggga
     2701 tggagatcgg gatgacggca gacgcggtcc gcgccggcta cacgctgcgc gagtacgaaa
     2761 tcgacctcga gcaccgcgcc accgggcgca gcctcgccgg cttccggcac cggggcgtac
     2821 agctgcgcga cttcctgcgg gtgtatttat cccgtatggg aggcaggggg agcgggcgat
     2881 gatcctggcg atcgaccagg ggacgacggg cacgacctgc ctcgtcttcg accgcgaggg
     2941 gcggctggtc gggcgcgcct actcggagtt cgagcagttc ttcccgcggc cgggctgggt
     3001 cgagcacgac gccaacgaga tctgggaagt gacccggcgg gtcgccgccg aggcgatcgc
     3061 ggcggccggg atcgaggggc gcgagctcga ctcgatcggg atcaccaacc agcgcgagac
     3121 cgtggtcgcc tgggatccgg cgagcggcga gccggtccac cgggcgctgg tctggcagga
     3181 ccggcgcacc gccgagcgct gcgacgagct gcgcgccgcc ggccacgagg cgctggtccg
     3241 cgagcgcacc ggcctcgtcg tcgacgccta cttctccggc accaagatcg agtggctgtt
     3301 ggccaacgcc gagggggccg agcgcgccgt cttcggcacg attgattcct ggctgctcta
     3361 caagctgacc ggccgccacg tcaccgacta ctcgaacgcg gcgcggacga tgctcttcga
     3421 catccgcaag cttgcctggg acgcggagct ctgcgcgctg ctcggggtcg acccggcgcg
     3481 gctgccggag ccgctgccct cggctggcag cttcggcacc accaccgagt tcggcggcga
     3541 ggtgccggtc tgtggggtcg ccggcgacca gcaggcggcg ctcttcggcc aggcctgcca
     3601 cgcgcccggt gacgccaaga acacctacgg caccggcagc ttcgtcctgc tcaacaccgg
     3661 cgccgaggcg ccgccgccgc ccgacggcct gctgaccacg gtcgcctggg ggctcggcga
     3721 cgaggtcgcc tacgccctgg aggcctcgat cttcgtcacc ggcgccgccg tgcagtggct
     3781 gcgcgacggg cttgggatca tcgacgcggc ggcggagacc gaggcgctcg ccgcctcgct
     3841 cgacaccaac gacggcgtct acttcgtgcc ggcgctgacc ggtctcggct cgccgcactg
     3901 ggacccctac gcgcgcggca cgatcgtcgg cctcacccgc ggcagcggcc gcgctcacct
     3961 ggcgcgggcg gcgctggagg gaatcgccta cgagacggtc gacgcggtgc gcgcccagga
     4021 ggccgcttcg ggcgtgcggc tcggcgcgct caaggccgac ggcggagcgg tcgccaaccg
     4081 ctggctgatg cagttccagg ccgacgtgct cggcgtcccc gtggtcgtcc ccgagatcgc
     4141 cgagacgacc gcgctcggcg ccgcctacct ggccggcgtc gccgccgggt tgtggacgcg
     4201 cgagcaggtg ggggagatgt ggcgcgaggc ggcccgctac gagccggcga tgggcgcgga
     4261 cgagcgccag accctgctca ccggctgggc ccgcgccctc gagcgctcgc gcgcctgggc
     4321 ctagcccgat cggcgggttt gatttcgcgt aaccggctgg aaatggaagg gttgttctaa
     4381 tcgatggccg agatgaagcc acaggtctac ctggacccgc gccccccgga gtacttcgac
     4441 cagttccacc gctcggcccg caagggctcg gtctggatgt actcgatcgc gcgggtggtc
     4501 ctcaccccac tggtcctgct gatctaccgg gtcaaggcgg tcgacaagga gaacgtgccg
     4561 cgcgagggcg cgctggtcct cgcccccaac cacttcagcc agctcgacca cttcttcgtc
     4621 ggcgtctggc tgcggcgcaa ggtccgcttc atggccaaat cccagctctt cggcccaccc
     4681 gtcctcacct tcgtcttcaa gcacggcggc gtcttcccgg ttcgccgcgg ccaccgcgac
     4741 gaagaggcgt tcaagaccgc ctacgagctg atcgaccaga aggagatgct gctggtctac
     4801 gccgagggcg gccgctcacg ctccggcgag atgggcgagc cgaaaccggg gatcggccgg
     4861 attgcgctgg agtccggcgt cccgatcgtc ccggtcgcga tttacggctc cgccgaagcg
     4921 cgccgctgga agcgcttccg cttccccaag atcacgatcc agttcggcga gcccatggtt
     4981 ttcccggtcg gtgccgaccc gagccgcgag cgccagctcg aggtcgccca cgcggtcttc
     5041 gccgacgtga agcggatgta cgaagggctc gctactcgta gcgaggctga agccgggggt
     5101 agaactccgg atcgtgcccg ggcgtaatca ccgcgtccgg gaactgcgag tggaacagtt
     5161 tcagctcctg cagggagcgg cggtagttgt gcgcgtcttc ggggcgggga ggcagcgccg
     5221 ctttgccgag cagctggtcg agcatgtaca tcgcgtcgcc gccgatcacg aagtcgcgtt
     5281 tggcgaggcg gcagatcacc gacatgtggc cggcgctgtg gccgggggtg aaggcgagcc
     5341 ggaccgagcc gtcgccgaag aggtcgaagg tgcggccgaa gctggcgtag gagtcgatgt
     5401 tggcgcggtc gaaggagacc gtgcggtact cgaaggcgaa gtcgatgtgc tggcgccggt
     5461 agcccttcag ggatccgcct gagccggtcg tcgcctcgac ccactcgggc tcggagacga
     5521 cgaaggtcga gttggggaac tcggagatcg ccgaggtgtg gtcgatgtgc aggtgggtca
     5581 tcaccacgat cgggatctcg ttggggtcca ggccgcggtc gcgcagctgt gccgggacgt
     5641 cttcgcccgg tttcagcgcc ggcttgccga agcggttgga catcccgccg aagttctcct
     5701 tgccgtcgct gacgatcgac ggatgcaggc cggtgtcgac gaggatcgcg ccggcgctcg
     5761 ggtggcggat caggaaggcg gggaccggga cgacgttcgg ctcgctcttg aagagcagcg
     5821 cccgcagcag cttcagggtg aggaagcggc cgccggggct gaccatcgtc ttcatcggga
     5881 agtcgaccgt gccggcgacc atcggttcga ccgcgacggt tgcaccgggg gtgcccccgg
     5941 cgagcggctc gtgcagtggg tgcggttcga cgctgacctt catcagaggg ccagattacg
     6001 cagagcaact cgcctatagg cttcgcgggc gatgtctgaa agctcgcatt cgcaggtgtt
     6061 tcgggacggc ttgctggctg gccaggtagt cgcgctctcc ggcgcggggg ccggcctcgg
     6121 ccgcgagtcg gcgctggagc tgctccgcct cggtgccacc gtggtcggct gcggccgtcg
     6181 cagcgagccg ctcgaggaga ccgccgagct ggccgccgac ctgcccggca gcttcgagca
     6241 cgagtcgctc gacatccgcg aggaggagcc ggtcgggcgc ttcttcgacg gtctgctgga
     6301 gcgccacggc cgcctcgaca ccctggtcaa caacgccggc ggccagttcc tcagcccggc
     6361 cgaggcgatc tcgccgaagg gcttcaagac ggtgatcgac ctcaacgtcc tcggcacctg
     6421 gctgatgacc cacgccgccg cgaccaaggc cttcatcccc caggagggcg gcaaggtgct
     6481 gagcgtcacc ctctcgcccc acaacgggat gccgggcatg gtccactccg gtgccgcccg
     6541 cgccgcggtc gagaacatga tgcgcacgct ctcgatcgag tgggcgcgct tcgggatcaa
     6601 gacctgctcg ctggccgccg gccacttcgc gaccgagacg ctgctgacca agtacccgcc
     6661 ggaggtggtc gccaacgtcg cccgctcgat ccccctgcag cgcaccggcc gcgccgagga
     6721 gatggcctgg ctggtcgcct acctcgcctc gcccgcgggc gacttcttct ccggcaccac
     6781 ggtcaccctc gacggcgccc gcgacaactg ggccggcccc tggccgcccg aatcgatcgc
     6841 cggcgagggc ggcgcaccgc ccgcggagca aagaaagcga gattgacccc acctgggtgg
     6901 tgtcaaagcg gcgaggggtt caggccgcgc gcggctcgag tacgaccagc gggatctcgc
     6961 ggcctttgct gcggatcgcg tagtcctcgt agccgcggta tgagtgcacg gccatctccc
     7021 agaggcggtc gcgctcctcg ggagtcgcga cgcgggcgtg gaccgggcgg cgttcgctgc
     7081 cgatctggac cacggtgtcc gggtgggcca tgaggttgtg gtaccaggcc gggtgtttgg
     7141 ggaagccgcc tttggaggcg acgatcacca cgtcggggcc gtcgtcgacg tagagcaggg
     7201 gggaggtgcg cagcttctgg ctcttcgcgc cgacgtggtc gagcagcagc atcttgccgc
     7261 gcacgcccgg gaccgagtgc ccgatccggc cgccggtgtg gcggtagagg aacgtgtggg
     7321 cacccatgaa acggcgggtc gcaggccaga tcttgtccgc tacgtcgagg tagcgggggg
     7381 tgcgtctggc ctgcggcgcc gtcgtcgtca tctcaggtgt acatcccgcc gaacatgccg
     7441 ccggtcacgt tgaggacctg gccgt
//
LOCUS       DASRIG010000492         9470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_463571, whole genome shotgun sequence.
ACCESSION   DASRIG010000492 DASRIG010000000
VERSION     DASRIG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463571"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /gene="atpD"
                     /locus_tag="VFB52_13165"
     CDS             complement(<1..1203)
                     /gene="atpD"
                     /locus_tag="VFB52_13165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZO07331.1"
                     /translation="MAESNGASGTNVGRVEQVTGVVIDAVFPDQLPEIYSAVTIETTG
                     DGDGEPTQLVCEVQQHLGDDRVRAVAMDATDGIQRGAKVVDTGGPITVPVGDVTLGRI
                     FNLLGDPIDNGDEIAADVERWPIHRPAPKASDLTPTQEVLETGIKVIDLLAPYAKGGK
                     VGLFGGAGVGKTVLIQELIRNIAEEHEGLSAFVGVGERSREGNDLWLEMKESGVIDKT
                     MLVFGQMNEPPGARLRVALSGLTMAEFYREQGGQDVLLFIDNIFRFVQAGSEVSALLG
                     RMPSAVGYQPTLETEMGELQERITSTDRGSVTSIQAVYVPADDYTDPAPATTFAHLDA
                     TTNLSRAIVELGIYPAVDPLASTSRILDPRIIGDEHYNVAKEVKRVLQRYKDLQDIIA
                     ILGIDELPE"
     gene            complement(1245..2165)
                     /locus_tag="VFB52_13170"
     CDS             complement(1245..2165)
                     /locus_tag="VFB52_13170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZO07332.1"
                     /translation="MATQKEVKNRIASIKNINKITRAMEMVAAARLRRAEQRIDDLRP
                     YAEGIRKLTRQASAHAGAIPRVPVMAERENVQKVGILLVTGDRGLAGAFNSNIIREGM
                     RMAADVRTEGAEPSFAAVGKRGVSALTFRKQEVTGAYTGFTDRPAFANAREIGEALTA
                     RYVDEELDRVELVYNKFVSPLTQHVWRQTLLPLQQAEVTGEGADEEQDAGEETEAEKQ
                     QSKSEWSYEPDPEELLARLIPEYVTISVYRALLESAASELGARMTAMRNAAENAEQIM
                     DDLTLEMNRVRQSEITQQILEVVSGAEALG"
     gene            complement(2189..3823)
                     /gene="atpA"
                     /locus_tag="VFB52_13175"
     CDS             complement(2189..3823)
                     /gene="atpA"
                     /locus_tag="VFB52_13175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZO07333.1"
                     /translation="MEIKPDEIASILRERIEGLESGSADLTEVGTVLSVADGIARIHG
                     LDNVMALEMLDLPHGVTGLALNLEADNVGAVLFGEWDKIVEGDTVKRTGHLLEIPVGE
                     ALLGRMVDPLGRPLDNRGEVVTTETRPAEFKAPGVVERQPVEEPVQTGLMSIDGMIPI
                     GRGQRELIIGDRQTGKTAIAIDTIINNKDKDLICIYVAIGQRMSTVVQLKETLEENGA
                     MENTIIVAAPADEAAPIKFLAPYAGAAIAEHWLYNEQHSLAIYDDLTKHAYAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAVKLNDDLGGGSLTALPIIETQASDVSAYIPTN
                     VISITDGQIFLESDLFFSGVRPAINVGISVSRVGGNAQTKAMKKVAGKLRLDLSQYRD
                     LEAFAQFGSELDAETQKALTRGERLVELLKQKEREPLSVGDQVASIYSGTGGYLDRIK
                     VERVGEFLADLRVRLHTENAELLKRIEDTGKLEEDDEKALGAAIADFIDDFGPDFDEH
                     GDPLEAGESDRIRSAEEREAPARTAESSDEEAATPA"
     gene            complement(3842..4375)
                     /gene="atpH"
                     /locus_tag="VFB52_13180"
     CDS             complement(3842..4375)
                     /gene="atpH"
                     /locus_tag="VFB52_13180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZO07334.1"
                     /translation="MPEAAHVYAEALFEAGKDKGKLDSLQSQLAQFTDVVDRNQELQV
                     FLFSPYLSSADKQEGLGRAISGAEPELMNFLELLVEKHRMPEIFRIRREFDELWKKEN
                     RRLDVTVVSAVELDPSVVEKIGQEVERQTGEAVELSSRVDDGILGGIVLQVGNMVLDA
                     SIRSRLEKLRKTVATAA"
     gene            complement(4376..4954)
                     /gene="atpF"
                     /locus_tag="VFB52_13185"
     CDS             complement(4376..4954)
                     /gene="atpF"
                     /locus_tag="VFB52_13185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZO07335.1"
                     /translation="MDLLGTIIPLATEAAGEEEGGSFLVSPGLGLMIWTLVLFLITMW
                     VLSKLAFPKIQEALDRRAKVIAESIDAAERQRKESEELLAEYRGRLAEAREQADDIMA
                     RARKAADTAEAEATAAGREKREELVAQAKRDIEAETRRSLEQIRGEVADLTVLATEKV
                     TRKSLNADDQKRLVEEALAEVDFSALSGAEKN"
     gene            complement(4961..5230)
                     /gene="atpE"
                     /locus_tag="VFB52_13190"
     CDS             complement(4961..5230)
                     /gene="atpE"
                     /locus_tag="VFB52_13190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZO07336.1"
                     /translation="MDLTSLPIAEITDEGITDAGKAIALGVGAGLGSIGAGIGIGFIF
                     GKEIESVARQPEMKDELQSIRWLGFALTEAVAFYTFIFGLIAFFL"
     gene            complement(5247..6212)
                     /gene="atpB"
                     /locus_tag="VFB52_13195"
     CDS             complement(5247..6212)
                     /gene="atpB"
                     /locus_tag="VFB52_13195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZO07337.1"
                     /translation="MSAEQAVAGGPEGAPKSGGLSTKAKVFIGLGAYLGVAILMFLIF
                     GDEGKNEHFQPQNEFKLEPWITIKIGGVDLSINKAVVYLLLASILTIVTMLWISKRMQ
                     QKPNRVQVAIEVAYDMTKNTIAGGNLDTKMAARWFPFLAALFFWIWFSNMIGFLPLPV
                     NTEHTVDIFGVAVPSFALYAATANLSIPLVLTLVVWVSYHVEGIRAKGFFKYFASWLP
                     PGLEDMNPAFKAFIFVIEAISHFARLISLSVRLFANILAGHLLLLFMGGGLAVLLGIA
                     ALGALTFPLAFFFFILEVGLIATLQAFIFSTLTAIYLGGATSASH"
     gene            complement(6209..6517)
                     /locus_tag="VFB52_13200"
     CDS             complement(6209..6517)
                     /locus_tag="VFB52_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07338.1"
                     /translation="MLGYAVGAAVWIIQRLVQVFAAKRATAELAAGNRQKAMGIVAAT
                     TLGRVWLMATVVLVLGLTADKEDGLSAAILVLTFFTVTFVVQGLTHLFGHEPEGQGVA
                     "
     gene            complement(6700..7965)
                     /locus_tag="VFB52_13205"
     CDS             complement(6700..7965)
                     /locus_tag="VFB52_13205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZO07339.1"
                     /translation="MTQVPDPWDALYAFLVAGALTLALVPLTIRLARWLGAIDFPNER
                     SLHAVPTPKLGGLAILVGVLVAGLIWLPDDTESHAILIGAVFVVLFGVLDDVFELGPV
                     PKLIGQAVSVVIPVSVGVTVDDFTFPFFGRVEPGSVELLTLPGGGVVDLGQVLTVLGI
                     VAVINVINLLDGVDGLAAGVCVISALTLSVIALSLDRTAAGVLAALVAGSSLGFLRYG
                     FPPASTFLGDTGSNLLGYLMGVIAVQGALKTNAVVALFLPLIVLAVPILDTGFVVAKR
                     LKYRRPIYRADRWHFHHRMADIGFSQRRTLVYLYGWTFVMALLALALRFVPYSDDHGE
                     FNTGWTIVMVVCGLLAIGASFYLVTVLEIFKLRQVRLRQLIDVRRQVGAPEPEPQEVN
                     AAVEAELETGSFPTIDPETGEFGAIDSRD"
     gene            complement(7962..>9470)
                     /gene="asnB"
                     /locus_tag="VFB52_13210"
     CDS             complement(7962..>9470)
                     /gene="asnB"
                     /locus_tag="VFB52_13210"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZO07340.1"
                     /translation="MFAIAIWDAAERRLLLARDRFGIKPLYYRHVGAELSFGSELKAL
                     IQQPGFSRAIDHEALAAYLTFNSIPAPLTIFAEARKLPQGHVLTWEAGQTRQRRYARP
                     GPPPASAQREGSADLLAAELREVLADSIRAHLVADVPVGVLLSGGVDSGGITALASAA
                     AEQPLKSFSIGFEEAGFDELDRARLVAQRYGTDHHELVLRPDAVELFPKLVEAFDEPF
                     GDSSALPTFLVSELAAGEVKVALSGEGGDELFGGYYTYVADLLAPRVGRLAALAAPLA
                     ERLPSGDRRVGFDYKAKRFSRAAHLPPLEAHHAWKEIFSPDTRAALLGADNPAWDPVD
                     LYRERYAETEGAESLSRLQDVDLGIYMVDDLLVKTDRSSMAHSLETRVPFLDQKVADF
                     ALSLPRPLRVRGFAKKRLLRQALAPLLPKEIVGGRKQGFSIPVAAWLRGPMQPFAREV
                     LSVANLERQGCLDPTTVQGVLDDHTSGRQDLSRQLWGLLAFTLWFDRYAEPR"
BASE COUNT         1511 a         3039 c         3361 g         1559 t
ORIGIN      
        1 ttccggcagc tcgtcgatgc ccaaaatcgc gatgatgtcc tgcaggtcct tgtagcgctg
       61 cagcacgcgc ttcacttcct tcgccacgtt gtaatgctcg tcgccgatga tgcgcggatc
      121 gagaatccgc gaggtcgaag cgagcggatc gaccgccgga tagattccga gctcgacaat
      181 cgcgcgcgac aggttcgtgg tcgcgtcgag atgcgcgaag gtcgtcgccg gagccgggtc
      241 ggtgtagtcg tccgccggca cgtagacggc ctggatcgag gtgacggagc cgcggtcggt
      301 cgaggtgatc cgctcctgca gttcgcccat ctcggtctcc agcgtcggct ggtagccgac
      361 cgcggagggc atccgcccga gcagggccga gacctcggag cccgcctgga cgaagcggaa
      421 gatgttgtcg atgaagagca gcacgtcctg gccaccctgc tcgcggtaaa attcggccat
      481 cgtcagcccg gagagagcga cgcgaagacg ggcgcccggg ggctcgttca tctggccgaa
      541 gaccagcatc gttttgtcga tgacgccgct ttctttcatc tcgagccaga ggtcgttgcc
      601 ctcgcgcgaa cgctcgccga cgccgacgaa ggcggagagg ccctcgtgct cctcggcgat
      661 gttgcggatc agctcctgga tcagcacggt cttgccgacg ccggcaccgc cgaacagccc
      721 gaccttgccg cctttggcgt agggcgccag caggtcgatg accttgatcc cggtctccag
      781 gacctcctgg gtcggggtca ggtcggaggc cttcggagcc gggcggtgga tcggccagcg
      841 ctcgacgtcg gcggcgatct cgtcgccgtt gtcgatcggg tcgccgagca ggttgaagat
      901 gcggccgagg gtgacgtcgc cgaccgggac cgtgatcggg ccgccggtgt cgacgacctt
      961 ggcgccgcgc tggatgccgt ccgtcgcgtc catcgcaacc gcgcgcacac ggtcgtcgcc
     1021 gaggtgctgc tgcacctcgc agaccagctg ggtcggttcg ccgtcgccgt cgcccgtggt
     1081 ctcgatcgtc accgccgagt agatctccgg cagctggtcg gggaagacgg cgtcgatcac
     1141 cacgccggtg acctgctcga cgcgccctac gttcgtaccg cttgctccgt tgctctcagc
     1201 catgacttcc ttttcctagt tccaaagggt ttctcgcgtc tcgtctaacc aagcgcctcg
     1261 gcgccggaga cgacctccag gatctgctgc gtgatctccg actgacggac ccggttcatc
     1321 tccagggtca ggtcgtccat gatctgttcc gcgttctcgg cggcgttgcg catcgcggtc
     1381 attcgcgccc cgagctccga cgccgccgac tcgagcagcg cccggtagac ggagatcgtc
     1441 acgtactcgg ggatcaggcg ggcgagcagc tcctcggggt cgggctcgta gctccactcc
     1501 gatttcgact gctgcttttc cgcctcggtt tcctcgccgg cgtcctgctc ctcgtcggcg
     1561 ccctcgccgg tcacctcggc ctgctgcagg ggcagcaggg tctggcgcca gacgtgctgg
     1621 gtcagcggcg agacaaattt gttgtagacg agttcgaccc ggtcgagctc ctcgtcgacg
     1681 tagcgagcgg tcagcgcctc gccgatttca cgcgcgttgg cgaaggcggg ccgatcggtg
     1741 aagccggtgt aggcgccggt cacctcctgc ttgcggaagg tcagcgccga gacaccacgt
     1801 ttgccgacgg cggcgaagga gggctcagcg ccctcggtgc ggacgtcggc cgccatccgc
     1861 atgccctcgc ggatgatgtt cgagttgaag gcgccggcca ggccgcggtc gccggtgacc
     1921 agcaggatcc cgactttctg cacgttctcg cgctcggcca tcaccggcac gcgcgggatc
     1981 gccccggcgt gggcagaggc ctggcgggtc agtttgcgga tgccctcggc gtagggccgc
     2041 agatcgtcga tccgctgttc ggcgcggcgc aggcgtgccg ccgcgaccat ctccatcgcg
     2101 cgcgtgatct tgttgatgtt tttgatcgac gcgatgcgat ttttgacttc cttctgcgtg
     2161 gccatggtgg tcgggtcctc cggtccggtt aggccggggt tgccgcctcc tcgtcgctgc
     2221 tctcggcggt gcgggccgga gcctcgcgct cctcggccga cctgatccgg tcggactcgc
     2281 cggcctccag cgggtcgccg tgctcgtcga agtcggggcc gaagtcgtcg atgaagtcgg
     2341 cgatcgcggc gccgagcgcc ttttcgtcgt cctcctcgag cttgccggtg tcttcgatcc
     2401 gcttcagcaa ctcagcgttc tcggtgtgca ggcggacccg caggtcggcg aggaactcac
     2461 cgacgcgctc gactttgatc cggtcgaggt agccgccggt gcccgagtag atcgaggcga
     2521 cctggtcgcc gacgctcagc ggttcgcgct ctttctgttt gagcagctcg accagccgtt
     2581 cgccgcgggt cagcgctttc tgcgtctccg cgtcgagctc ggagccgaac tgggcgaagg
     2641 cctcgaggtc gcggtactgg gagaggtcga ggcggagttt tccggcgacc ttcttcatcg
     2701 ccttggtctg cgcgttaccg ccgacccggg agaccgagat gccgacgttg atcgccgggc
     2761 ggacgccgga gaagaacaga tcggactcga ggaagatctg cccgtcggtg atcgagatca
     2821 cgttggtcgg gatgtaggcg gagacgtcgg aggcctgggt ctcgatgatc ggcagcgccg
     2881 tcagcgaacc gccgccgagg tcgtcattga gcttgaccgc gcgctccaag aggcgcgagt
     2941 gcaggtagaa gacgtcgccc gggtacgcct cgcggcccgg cgggcggcgc agcagcagcg
     3001 acatctgccg gtacgcgtac gcgtgcttcg tgaggtcgtc gtagatcgcg agcgagtgct
     3061 gctcgttgta gagccagtgc tcggcgatcg ccgcgcccgc gtagggcgcc aggaacttga
     3121 tcggcgccgc ctcgtcggcc ggagcggcga cgatgatcgt gttctccatc gcgccgttct
     3181 cctccagcgt ctccttgagc tggacgacgg tggacatccg ctgcccgatc gcgacgtaga
     3241 tgcagatcag gtccttgtcc ttgttgttga tgatcgtgtc gatcgcgatc gccgtcttac
     3301 cggtctggcg gtcgccgatg atcagctctc gctggccacg gccgatcggg atcatcccgt
     3361 cgatcgacat cagcccggtc tggacgggtt cctcgaccgg ctggcgctcg accacgccgg
     3421 gggctttgaa ctcggccggg cgggtctcgg tggtgacgac ctcgccgcgg ttgtcgagcg
     3481 ggcggccgag cgggtcgacc atgcgaccga gcagcgcctc gccgaccggg atctcgagca
     3541 ggtggccggt ccgtttgacc gtgtcgccct cgacgatctt gtcccactcg ccgaacagca
     3601 cggcaccgac gttgtcggcc tcgaggttga gcgccagccc ggtgaccccg tgcggcaggt
     3661 cgagcatctc gagcgccatc acgttgtcga ggccgtggat gcgggcgata ccgtcggcca
     3721 cggaaagcac cgtgccgacc tcggtgaggt cggcggagcc gctctccagc ccctcgattc
     3781 gctctcgcag aatgcttgcg atctcgtccg gcttgatctc catcgggtgc tcagctcctc
     3841 cttacgccgc ggtggcgacg gtctttctaa gtttctccag gcgactgcgg atgctcgcgt
     3901 ccaaaaccat gttcccgacc tgcagcacga tgccgccgag gatgccgtcg tcgacgcggc
     3961 tcgacagctc gaccgcctcg ccggtctgtc gttcgacctc ctggccgatt ttctcgacca
     4021 ccgacgggtc aagctcgacg gcgctgacca cggtcacgtc gaggcggcgg ttctccttct
     4081 tccagagctc gtcgaactcg cgccggatgc ggaagatttc cggcatccgg tgcttctcga
     4141 ccagcagctc gaggaaattc atcagctccg gctcggcgcc cgagatcgcg cgtccgagac
     4201 cctcctgctt gtcggccgag gagaggtagg ggctgaacag gaacacctgc agttcctggt
     4261 tgcggtcgac cacgtcggtg aactgggcaa gctgcgactg cagcgagtcg agcttgcctt
     4321 tgtccttgcc ggcctcgaac agggcctcgg cgtatacgtg ggcggcctcg ggcatctagt
     4381 tcttctccgc ccccgagagc gccgagaagt cgacctcggc gagggcctcc tcgacgagcc
     4441 gcttctggtc gtcggcgttg aggctcttgc gggtgacctt ctcggtcgcc agcacggtca
     4501 ggtcggcgac ctcgccgcgg atctgctcga gcgagcgacg cgtctccgct tcgatgtcac
     4561 gcttggcctg ggcgaccagc tcctcgcgtt tctcgcggcc ggccgcggtt gcctccgcct
     4621 cggcggtatc ggccgctttg cgggcgcggg ccatgatgtc gtcggcctgc tcgcgcgcct
     4681 cggccagacg gccgcggtac tcggcgagca gttcctcgga ctctttgcgc tggcgctcgg
     4741 cggcatcgat cgactcggcg atgactttcg cccgtcggtc cagcgcctcc tggattttcg
     4801 ggaacgcgag cttgctgagg acccacatcg tgatcaggaa gagcacgagg gtccagatca
     4861 tcagcccgag gccgggtgag acgaggaagc tgccgccttc ctcctcgccc gcggcctcag
     4921 tcgccagcgg aattatggtg ccgagcagat ccatcgtggc ctagaggaag aacgcgatca
     4981 ggccgaagat gaacgtgtag aaggccacgg cctcggtcag cgcgaagccg agccagcgga
     5041 tgctctggag ctcgtctttc atctccggct gacgggcgac cgactcgatc tccttaccga
     5101 agatgaagcc gatgccgata ccggcgccga tcgagccgag gccggcaccg acaccaaggg
     5161 cgatcgcctt gccggcgtcg gtaattcctt cgtcggtgat ctcggcgatc ggtagggacg
     5221 tgagatccat cgaggtgact ccttttctag tgactggcgg aggtagctcc gccgaggtag
     5281 atggcagtga gggtcgagaa aatgaacgcc tgcaaggtgg cgatcaggcc gacctcgagg
     5341 atgaagaaga agaaggcgag cgggaaggtg agcgcgccca gggccgcgat cccgagcagc
     5401 acggcgagcc cgccgcccat gaagagcagc agcaggtggc cggcgaggat gttggcgaaa
     5461 agtcggacgg agagcgagat caggcgggcg aagtgggaga tcgcctcgat cacgaagatg
     5521 aacgccttga aggcggggtt catgtcctcg aggccgggcg gcagccagct ggcgaagtac
     5581 ttgaagaagc ccttggcgcg gatcccttcg acgtggtagc tgacccaaac gacgagggtg
     5641 agcacgagcg ggatcgagag gttcgcggtc gcggcataga gggcgaagga ggggacggcg
     5701 acgccgaaga tgtccaccgt gtgttcggtg ttcaccggca gcggcaggaa cccgatcatg
     5761 ttcgagaacc agatccagaa gaagagcgcg gcgaggaagg ggaaccagcg ggccgccatc
     5821 ttggtgtcga ggttgccgcc cgcgatcgtg ttcttggtca tgtcgtaggc gacctcgatc
     5881 gcgacctgga cccggttcgg cttctgctgc atccgtttcg agatccagag catcgtgacg
     5941 atcgtgagga tgctggccag gagcaggtag acgaccgcct tgttgatgct gaggtcgacg
     6001 ccgccgatct tgatcgtgat ccagggctcg agcttgaact cgttctgcgg ctggaagtgt
     6061 tcgtttttgc cttcatcccc gaagatcagg aacatcagga tcgcgacgcc gaggtaggcg
     6121 ccgagcccga tgaagacctt ggccttggtg ctgagaccgc ccgacttcgg ggcgccctcg
     6181 ggccccccgg cgactgcctg ctctgcgctc atgcgacccc ctggccttcg ggctcatggc
     6241 cgaacaggtg cgtgaggccc tggacgacga aggtgaccgt gaagaaggtg aggaccagga
     6301 tcgcggccga gaggccgtcc tctttgtccg cggtgaggcc gaggacgagg acgacggtgg
     6361 ccatcagcca gacgcggccg agcgtggtcg cggcgacgat gcccatcgcc ttctggcgat
     6421 tgccggcggc gagctcagcg gtggcgcgct tggcggcgaa gacctggacg aggcgctgga
     6481 tgatccagac ggcagcgccg acggcgtagc caagcatcgg gaagtccccc aaaaggaata
     6541 cgacgagccc ggcggcaagg agcagaaggt cgacgtactt gggaggagac ataggtgacc
     6601 cgctctctgc gagcgggcaa ggcgcgacta tatcccaaag gtcccgggaa tggctttctg
     6661 atgcgggaaa gtgccgcttg tcacaacctg tcactaacgc tagtcgcggc tgtcgatcgc
     6721 gccgaactcc cccgtttccg ggtcaatcgt ggggaagctg ccggtctcca gctcggcctc
     6781 cacagcggcg ttgacctcct gcggctcggg ctcgggggcg ccgacctgcc ggcgcacgtc
     6841 gatcagctgg cgcagccgca cctgccgcag cttgaaaatc tccagcaccg tgaccaggta
     6901 gaagctggcg ccgatcgcga gcagcccgca gaccaccatc acgatcgtcc agccggtgtt
     6961 gaactcgccg tggtcgtcgc tgtaggggac gaagcgcagg gcgagcgcca gcaacgccat
     7021 cacaaaggtc cagccgtaga ggtagaccaa cgtgcggcgc tgggagaagc cgatgtcggc
     7081 catccggtgg tggaagtgcc agcggtcggc gcggtagatc gggcgccggt atttgagccg
     7141 cttggcgacg acgaagccgg tgtcgaggat cggcacggcg agcacgatca ggggcaggaa
     7201 gagggcgacg acggcgttgg tcttcagcgc cccctggacc gcgatcaccc ccatcaggta
     7261 gccgagcagg ttggagccgg tgtcgccgag gaaggtgctg gcgggcggga agccgtaacg
     7321 caggaagccg agcgaggagc cggcgaccaa cgccgccagc acgccggcgg cggtgcggtc
     7381 gagcgagagc gcgatcaccg acagggtgag ggcggagatc acgcagacgc cggcggcgag
     7441 cccgtcgacc ccgtcgagca ggttgatcac gttgatcacg gcgacgatcc cgagcacggt
     7501 cagaacctgg ccgaggtcga ccacgcctcc cccgggcagg gtcagcagtt cgacgctgcc
     7561 cggttcgacc cggccgaaga aggggaaggt gaagtcgtcg accgtgaccc cgaccgagac
     7621 cgggatcacc accgagaccg cctggccgat cagcttcggc accgggccga gttcgaaaac
     7681 gtcgtcgagc acgccgaaga ggacgacaaa aacggcgccg atcaggatcg cgtggctctc
     7741 ggtgtcgtcg ggcagccaga tcagaccggc gacgagcacg ccgacgagga tcgcgaggcc
     7801 gccgagcttc ggcgtcggca cggcgtgcag ggaacgctcg ttgggaaagt cgatcgcgcc
     7861 gagccaacgc gccaaccgga tcgtcagcgg caccagcgcc aaggtgaggg cgcccgcgac
     7921 gaggaaggcg tagagcgcgt cccagggatc cggcacctgg gtcatcgcgg ctcagcgtaa
     7981 cggtcgaacc agagggtgaa agcaagcagg ccccagagct ggcggctgag gtcctgtcgt
     8041 ccagaggtgt ggtcgtcgag cacaccctga accgtcgtcg ggtcgaggca gccctggcgc
     8101 tcgaggttgg cgaccgagag gacctcgcgc gcgaacggct gcatcgggcc acgcagccag
     8161 gcggcaaccg ggatcgagaa gccctgcttg cgcccgccga cgatctcctt gggcagcagc
     8221 ggcgcaagcg cctggcgcag cagccgcttc ttggcgaagc cgcgcacccg cagcgggcgc
     8281 ggcagcgaga gggcgaagtc ggcgaccttc tggtcgagga aggggacgcg ggtctccaac
     8341 gagtgagcca tgctcgaacg gtcggtcttc accagcaggt cgtcgaccat gtagatcccg
     8401 aggtcgacgt cctggaggcg ggacagcgac tcggcgccct cggtctcggc gtagcgctcg
     8461 cggtagaggt cgaccgggtc ccaggccggg ttgtcggcgc cgagcagcgc ggcgcgcgtg
     8521 tccggggaga agatctcctt ccaggcgtgg tgggcctcca gcggcggcag gtgggcggcg
     8581 cgcgagaagc gcttggcttt gtagtcgaag ccgacccggc ggtcgccgct cggcagccgc
     8641 tcggcgagcg gcgccgcaag cgcggcgagg cggccgaccc gcggggccaa gagatcggcg
     8701 acgtaggtgt aatagccgcc gaagagctcg tcgccgccct cgccggagag cgccaccttg
     8761 acctcgcccg cggccagctc ggagacgagg aaggtgggca gcgccgagga gtcgccgaac
     8821 ggctcgtcga aggcctcgac cagcttcgga aacagctcga ccgcgtcggg gcgcaggacc
     8881 agctcgtgat ggtcggtgcc gtagcgctgg gcgaccaggc gggcgcggtc gagctcgtcg
     8941 aagccggcct cctcgaaccc gatcgaaaac gatttgaggg gctgctcggc ggcggccgag
     9001 gcgagcgcgg tgatcccgcc ggagtcgacg ccgccggaga gcagcacgcc gaccgggacg
     9061 tcggcgacca ggtgggcgcg gatcgagtca gccagcacct cgcgcagctc ggcggcgagc
     9121 aggtcggcgg agccttcgcg ctgggcggag gccggcggcg ggccggggcg cgcgtagcgg
     9181 cgctgccggg tctgcccggc ctcccaggtc aggacgtggc cctggggcag cttgcgcgcc
     9241 tcggcgaaga tcgtcagcgg cgccgggatc gagttgaagg tgaggtaggc ggcgagcgcc
     9301 tcgtggtcga tcgcgcgcga gaagccgggc tgctgaatca gggccttgag ctccgagcca
     9361 aacgagagct cggcgccgac gtggcggtag tagagcggct tgatcccgaa gcggtcgcgg
     9421 gcgagcagca gccgccgctc cgcggcgtcc cagatcgcga tcgcgaacat
//
LOCUS       DASRIG010000493         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3618553, whole genome shotgun sequence.
ACCESSION   DASRIG010000493 DASRIG010000000
VERSION     DASRIG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618553"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFB52_13215"
     CDS             complement(<1..242)
                     /locus_tag="VFB52_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07341.1"
                     /translation="MRRPFAKREPAARQSTKPVPDERIWGRHYHGPVPWVFGLAIVVL
                     LAIGVYLAFAKELPFGDKGYELTATFENAATLRETSP"
     gene            complement(239..1702)
                     /locus_tag="VFB52_13220"
     CDS             complement(239..1702)
                     /locus_tag="VFB52_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO07342.1"
                     /translation="MRRGVASGLAASPTMVGAVTVMIIIVAVFLAYNANTGLPFVPSY
                     RISAEVPNASSLVPGNEVRIGGVRVGLVEAIEPQAHDDGTVSAVLDLKLDADLEPLPD
                     DSTMIIRARSALGLKYLEIAKGSSSEGYAQGATVPLSSYGSPDPDDPSVLEEPVEIDQ
                     VLSTFDAPTRRAIQENLVEFGNALAGRGPALNSALGRLPRVLRYLEPVMTNLSAPDTR
                     LERFISASAAAAAEVAPVAEVQAELFVNLDITFTALAGVARPFIQETISETPPTFAVA
                     ERALPTIRPFLDHSAELFTELEPGVDALARYAPPISETLETGTPVLSDAPRLNRELVP
                     TSEALLAFNNDQSVRNGLARLNQTVDIAGPALRFITPAQTVCNYASLFLGNLAGVTAE
                     GGVGTKWQRFSVFQPPTGPNNEGGVASAPANGGGNPANFLHFNPYPNTAAPGQPNECE
                     AGNEPYLGGQQTIGNAPGNQGTVTGGQPGSDESEEEE"
     gene            complement(1699..>2007)
                     /locus_tag="VFB52_13225"
     CDS             complement(1699..>2007)
                     /locus_tag="VFB52_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07343.1"
                     /translation="TAAAGPGRDREHQRDRRRGPEQEGAGTRRLSQSADGQAFEVTPE
                     GLDLEPGELDLDAAPAAPEAAPPTAGQMRATRDLLDFLIGARSADGRSKIRRDDGGER
                     "
BASE COUNT          269 a          696 c          724 g          318 t
ORIGIN      
        1 ggagaggtct ctcgcagggt ggcggcgttc tcgaacgtgg ccgtcagctc gtagcccttg
       61 tcgccgaacg gcagctcctt ggcgaacgca agatagaccc cgatcgcgag cagcacgacg
      121 atcgccaggc cgaagaccca cggcaccggg ccgtggtaat ggcggcccca gatccgctcg
      181 tcgggcaccg gcttggtcga ctggcgcgcc gccggctctc gctttgcgaa cgggcgcctc
      241 actcctcctc ctcggactcg tcggagccgg gctgaccacc ggtcaccgtg ccctggttgc
      301 cgggcgcgtt gccgatcgtc tgctggccac ccaggtaggg ctcgttgccg gcctcgcact
      361 cgttcggctg gccgggcgcc gcggtgttcg ggtaagggtt gaaatggagg aagttcgcgg
      421 ggttcccgcc gccgttggcc ggcgccgagg cgacgccacc ctcgttgttg ggacccgtag
      481 gcggctggaa caccgagaac cgctgccact tcgtgccgac accgccctcg gcggtcaccc
      541 cggcaaggtt gcccaggaac aggctcgcgt agttgcacac ggtctgggcc ggggtgatga
      601 agcggagcgc cgggccggcg atgtccaccg tctggttcag gcgcgcgaga ccattgcgca
      661 cgctctggtc gttgttgaac gcgagcagcg cctccgacgt gggtaccagc tcgcggttga
      721 gccgcggtgc gtcgctcagg accggcgtcc cggtctcgag cgtttcggaa atcggcgggg
      781 cgtagcgcgc gagcgcgtcg acccccggct cgagctcggt gaacagctcg gcgctgtggt
      841 cgaggaacgg ccggatcgtc ggcagcgcgc gctcggcgac tgcgaaggtc ggtggagtct
      901 cggagatcgt ctcctggatg aacgggcggg cgacccctgc gagcgcggtg aaggtgatat
      961 cgaggttgac gaacagctcg gcctgcacct ctgccaccgg cgccacctcg gcggccgcag
     1021 cggcgctggc gctgatgaag cgctcgagcc gggtgtccgg cgccgaaagg ttggtcatca
     1081 ccggttcgag gtagcgcagc acccgcggca gcctcccgag cgccgagttg agggccggcc
     1141 cgcgcccggc gagcgcgttg ccgaactcaa ctaggttctc ctggatcgct ctgcgcgtgg
     1201 gcgcgtcgaa ggtgctcagc acctggtcga tctcgaccgg ctcctcgagc acgctcgggt
     1261 cgtcggggtc cggtgacccg taggaggaga gcggtaccgt cgctccctgc gcgtatccct
     1321 cgctcgagct gcccttggcg atctcgaggt acttgagccc gagcgccgag cgagcgcgga
     1381 tgatcatcgt cgagtcgtcg ggcagcggct cgaggtcggc gtcgagcttc aggtcgagaa
     1441 cggcgctgac ggtgccgtcg tcgtgggcct gcggctcgat cgcctccacc aacccgaccc
     1501 ggacgccacc gatacggacc tcgttgccgg gcacgaggga gctcgcgttc ggaacctcgg
     1561 cggagatccg gtaggacggc acgaacggca gcccggtgtt cgcgttgtag gccaggaaga
     1621 cggcgacgat gatgatcatc accgtcaccg ccccgaccat ggtcgggctc gcggcgagtc
     1681 cggaggcgac tcctcgcctc atcgctcgcc cccgtcgtct ctcctgatct tgctccggcc
     1741 gtcggccgac cgggcgccga tcaggaagtc gagcaggtcg cgggtagccc gcatctgccc
     1801 agcggtcggc ggggcggcct cgggcgccgc gggcgccgcg tccaagtcga gctcccccgg
     1861 ctccaggtcc aggccttcgg gcgtgacctc gaacgcctgc ccatcggcgc tctgcgacag
     1921 ccgccgggta ccggcaccct cctgctcggg acctcgcctg cgatcgcgct ggtgttcgcg
     1981 atcacgcccg gggccggccg ccgcggt
//
LOCUS       DASRIG010000494         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3619549, whole genome shotgun sequence.
ACCESSION   DASRIG010000494 DASRIG010000000
VERSION     DASRIG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3619549"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFB52_13230"
     CDS             <1..203
                     /locus_tag="VFB52_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07344.1"
                     /translation="WGMIGGLSHNIVNRYEDGDTTILESEVTYTRQDGEQVVIKTVSL
                     LHRDGDKVDRLAFYNDPSPIFA"
     gene            213..965
                     /locus_tag="VFB52_13235"
     CDS             213..965
                     /locus_tag="VFB52_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07345.1"
                     /translation="MSNSHEGRTALVTGAAAGIGQAYAKRLAEDGANVVVADISDGAE
                     TVGLIEAAGSQGLAVECDVSSAEAVAAAAAAAQERFGTVDILVHNAGIYPIQDFASMS
                     FEDWRQVLSVNLDSAFHLCHELLPGMREQEWGRVVLIASNTFHAGIGNMAHYVASKGG
                     LIGFLRSLADEVGNEGVTVNGVAPSLVRSKGTIEGPHEELGLFEFVAQGQAIKRTQEP
                     EDLVGAISFLTSDDAAFITGQTLCVDGGWVRA"
     gene            1096..2163
                     /locus_tag="VFB52_13240"
     CDS             1096..2163
                     /locus_tag="VFB52_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07346.1"
                     /translation="MSETSTTPQAVATSPNSAGTPTPPPAASIGDRVRAGIGRAFQVD
                     ELGPLLALIALVVFIGGFHSDFLDKDVLLNTVRSAAFVAIVAYGMVYLLAMTEIDLSV
                     GGIYALCIMLAAKWMSSIDPWVAAMLAICVGIALGAVNGILCNLFKIPVIIITLGTLS
                     MFRGLVQIVSSGKPATGIPLDSSFFEKLGGDILGIPAAAWVVVVLGVILTFVFKKTRF
                     GAMVRAVGSNHQAAEFSGIPVLRIRLYALMLVGGLAGLAGVLSLAYFQGADPTVGAGF
                     ELQVIAAAIIGGTAVSGGTGTVPGALIGALIVAVINSGLIFFSIDSNWTNFVTGAVIV
                     IAVGSDALVRRRRAARALQGT"
     gene            2213..>3018
                     /locus_tag="VFB52_13245"
     CDS             2213..>3018
                     /locus_tag="VFB52_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07347.1"
                     /translation="MSNKRSNKGLLVLAALAALLLALGLAACGGDDDSSSSGGSESTE
                     TTGGGEGGGEEKEQVSIAMVEILTGVSFGQEIAEGMKGFAAEDGAVDLTVQGPPTVEP
                     EVAQKMAADLLATSPDAMGVAPFPPELWTRTMKNITEQVDNYLVFNERPSSLPEDVSA
                     AAIQLFVGTDDKTEAREMLEETIKLAKIPKSSTGKVILGQCVAQEAGVLAQRTEGFDE
                     ALAALLPNAQVEKFTSEVEPQKNTKAWTTELAANPDAMLATGTCDQDGTSL"
BASE COUNT          537 a         1024 c          982 g          475 t
ORIGIN      
        1 tctggggcat gatcggcggc ctcagccaca acatcgtcaa ccgctacgag gacggcgaca
       61 cgacgatcct cgagtccgag gtcacctaca cccgccagga cggcgagcag gtcgtgatca
      121 aaaccgtctc gctgctgcac cgcgacggcg acaaggtcga tcgactcgcc ttctacaacg
      181 acccctcgcc gatcttcgcc taggcgccga cgatgagcaa ctcacacgag ggacgcaccg
      241 ccctggtcac cggggcggcc gccgggatcg ggcaggccta cgcgaagcgg ctggctgagg
      301 acggcgccaa cgtcgtcgtc gccgacatct ccgacggtgc cgagaccgtc ggcctgatcg
      361 aggcggccgg ctcgcagggg ctggcggtcg agtgcgacgt ctcctcggcc gaggcggtcg
      421 ccgccgcggc tgcggcggcg caggagcgct tcggcaccgt cgacatcctc gtccacaacg
      481 ccgggatcta cccgatccag gacttcgcga gcatgagctt cgaggactgg cgccaggtgc
      541 tctcggtcaa cctcgactcg gccttccacc tctgccatga gctgctgccg ggcatgcgcg
      601 agcaggagtg ggggagggtg gtcctgatcg cctccaacac cttccacgcc ggaatcggca
      661 acatggccca ctacgtcgcc agcaagggcg gcctgatcgg gttcctgcgc tcgctcgccg
      721 acgaggtcgg caacgaaggc gtgaccgtga acggggtcgc cccctcgctg gtccgctcga
      781 agggcacgat cgagggtccg cacgaggagc tcggcctgtt cgagttcgtc gcccagggcc
      841 aggcgatcaa gcgcacccag gagccggagg acctggtcgg ggcgatctcc ttcctcacct
      901 ccgatgacgc cgccttcatc accgggcaga cgctctgtgt ggatggcggt tgggtcaggg
      961 cctgacgtac cgaaaagtca aatctccggc cggatcaatt ccggcccggg ggttgacaac
     1021 cactacgaat caatatattg attacgagta acgtaatagg cgccgtcgtg gggacggcga
     1081 cgggaatggg atgagatgag cgagaccagc accacgccgc aagcagtcgc aacgtcgccg
     1141 aactcggccg gcaccccgac gccgcccccg gccgcctcga tcggggaccg cgtgcgcgcc
     1201 ggcatcggcc gcgccttcca ggtcgacgag ctcggcccgc tgctggcgct gatcgccctg
     1261 gtcgtcttca tcggcggctt ccactccgac ttcctcgaca aggacgtcct gctcaacacc
     1321 gtccgctccg cggccttcgt cgcgatcgtc gcctacggga tggtctacct gctggcgatg
     1381 accgagatcg acctctcggt cggcggcatc tacgcgctct gcatcatgct cgcggcgaag
     1441 tggatgtcga gcatcgaccc gtgggtcgcg gcgatgctcg cgatctgcgt cgggatcgcg
     1501 ctcggggcgg tcaacggcat cctctgcaac ctcttcaaga tcccggtgat catcatcacc
     1561 ctgggaacgc tctcgatgtt ccgagggctg gtccagatcg tctccagcgg caaaccggcg
     1621 accggcatcc cgctcgactc gagcttcttc gaaaagctcg gcggcgacat cctcgggatc
     1681 cccgccgcgg cctgggtggt cgtcgtctta ggggtgatcc tcaccttcgt cttcaagaag
     1741 acgcgctttg gtgcgatggt gcgcgcggtc ggctccaacc accaggcggc cgagttctcc
     1801 gggatcccgg tgctgcgcat ccgcctctac gcgttgatgc tggtcggtgg cctcgccggg
     1861 ctcgccggcg tgctcagcct cgcctacttc cagggcgctg acccgaccgt cggcgccggc
     1921 ttcgagttgc aggtgatcgc cgccgcgatc atcggcggca ccgcggtcag cggcggcacc
     1981 gggaccgtgc ccggggccct gatcggagcg ctgatcgtcg ccgtcatcaa ctccggactc
     2041 atcttcttct cgatcgattc caactggacg aacttcgtca ccggtgcggt gatcgtgatc
     2101 gcggttggaa gcgacgcact cgtccgacgc cggagggccg cgcgcgcctt gcagggcacg
     2161 tagcggactt ccatcagggg ttcaccacaa aaaagaggag aggtatcact gcatgagtaa
     2221 caagcgatcc aacaaggggc tgctcgtctt ggccgccctc gcggcgctgc tcttggccct
     2281 cgggctcgca gcgtgcggag gcgacgacga ctcgtcgagc tccggtggtt cggaaagcac
     2341 cgaaaccacc ggtggcggtg aaggcggcgg cgaagaaaaa gaacaggttt caatcgcgat
     2401 ggtggagatc ctgaccggcg tcagcttcgg ccaggaaatc gccgaaggga tgaaaggctt
     2461 cgccgctgaa gacggcgccg tcgacctcac ggtccagggc ccgccgacgg tggaacccga
     2521 agtcgcgcag aagatggcgg ccgacctcct ggccaccagc ccggacgcga tgggcgtcgc
     2581 cccgttcccg cccgagctct ggacgcggac gatgaaaaac atcaccgaac aggtggacaa
     2641 ctacctggtc ttcaacgaac gccccagctc gctgccggaa gacgtctcgg ccgcggcgat
     2701 ccagctcttc gtcggcacgg atgacaaaac cgaggcccgc gaaatgctcg aagaaacgat
     2761 caaactggcg aaaatcccga aatcgagcac cggcaaggtg attctcggcc agtgcgtcgc
     2821 ccaggaagcg ggcgtgctcg cgcagcggac cgaagggttc gacgaagcgc tggccgcgct
     2881 gctgccgaac gcccaggtgg agaagttcac ctcggaagtc gaaccgcaga aaaacaccaa
     2941 ggcgtggacg accgagctcg ccgccaaccc ggacgcgatg ctggccaccg gtacctgtga
     3001 ccaggacggc accagcct
//
LOCUS       DASRIG010000495         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2555188, whole genome shotgun sequence.
ACCESSION   DASRIG010000495 DASRIG010000000
VERSION     DASRIG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555188"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VFB52_13250"
     CDS             <1..1193
                     /locus_tag="VFB52_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZO07348.1"
                     /translation="VGSALADEDGVTCDMTLMRGVEIDFDTKVVHVQGGCKLGDVDEA
                     TAEHGLAVPAGIVSDTGVPGLALGGGIGWLSRKHGLTCDNFVSLEVVTAAGETIEVSA
                     DSHPDLFWALRGGGGNFGVVTRFSFQAYDFGPTMRFGAALYHEDEARAALREYARIYP
                     SLPNVVGWHVAMKREMPPLPFVPEELVGTPLVMLFCMWLDDPESEEGVAMVERLTSIG
                     DPAVVGSAVIPFGLGMQRFLDAEFPDGLRNYTKEGHLEELSEGAIDALVDFWAQSLNS
                     NTTIEGELAIYGLGGVAREVPEMDAAFSNRDSLWWINWANHWHDAVDDERNMEIIRAS
                     YRSIEPWLGKGVYVNMLNVDELDRVVEAYGGPEKYARLGRVKATYDPTNLFRMNHNIK
                     PVDA"
     gene            1190..2122
                     /locus_tag="VFB52_13255"
     CDS             1190..2122
                     /locus_tag="VFB52_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZO07349.1"
                     /translation="MNGESLPTRVGVAKLGGGPELLAAYGDRVVSLTAALGWNHASTA
                     ELIGRWDELSPRLREIGESAPEVSVEDAAWEPPVMPAKLICVGANYTDHVEEMEKAGA
                     PKVEATAYPFSFLKPPSTALAGSGVEVAMPGYATELDWEAELAVVIGRPELAADDPLG
                     AIFGYSNLNDLSLRDFVAPFPHPLGLDAVIGKGWDGAAPMGPWITLASEAGDPEHMSI
                     ETRVNGAVKQQSSTEKMIFTVAEMVAYYARVLTLEVGDVIATGTPAGVGAGAQPPEFL
                     APGDTVEVEIGGLGTLSTPIVAPRSAVSLDIHQV"
     gene            2150..3349
                     /locus_tag="VFB52_13260"
     CDS             2150..3349
                     /locus_tag="VFB52_13260"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HZO07350.1"
                     /translation="MAKVLCVLYDDPVDGYPPAYARDDIPKIEGYPDGQSAPAPNGID
                     FTPGELLGCVSGELGLRPYLEAQGHELIVTSDKDGPGSEFEKHLADAEIVISQPFWPA
                     YLTAERIAKAPSLKIAITAGIGSDHVDLQAAMDAGITVAEVTYCNSNSVAEHVVMMIL
                     GLVRNYIPSYKIVVEGGWNIADAVSRSYDVEGMQVGTVAAGRIGLGVLRRMAPFDVGL
                     HYMDRHPLPPEVEKELNATHHETVESLVEVCDVVTINCPLHPETEHLFDEEMIARMKR
                     GAYLINTARGKICDRDAVVRALESGQLAGYAGDVWFPQPAPQDHPWRTMPHHGMTPHV
                     SGSSLSAQARYAAGTREILECYFEGRPQREEYLIVDGGGLAGTGAHSYSAGNATGGSE
                     EAAEFKG"
     gene            complement(3402..4274)
                     /locus_tag="VFB52_13265"
     CDS             complement(3402..4274)
                     /locus_tag="VFB52_13265"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07351.1"
                     /translation="MSRVAFVGLGLMGRPMAENLLAAGHDVVVANRSPEPVAALEAAG
                     AEGAADAAAAARGADAAITMLPDGPTVHALMTGEEGILAALPEGALAIDMSTIAPAEA
                     RLLAAAGAERGIGVLDAPVSGGDVGARAGTLTIMVGGEREDFERALPLFEAMGTSVTH
                     VGPAGAGQVVKACNQVAVALILEGLVEALTLGAAAGVDPETILDVFAGGMVGGRMLEA
                     RGPALLSREHEPGFRLEHHLKDLAIALAEAKDRGVELPATPVVKQLMEQLHAEGRGAE
                     NHTALIDAVERRAG"
     gene            complement(4271..4606)
                     /locus_tag="VFB52_13270"
     CDS             complement(4271..4606)
                     /locus_tag="VFB52_13270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZO07352.1"
                     /translation="MDEPEPNEEEARMAYVVIAKWTAQEGNEEAVAGVLAQMVEPTRA
                     EPGNLAYVIHRDPEDPRVFLLYEQYADEGAYAAHGASEHFQRLAVGQGFDLLDSRERE
                     FYATWEAGS"
     gene            complement(4599..>4986)
                     /locus_tag="VFB52_13275"
     CDS             complement(4599..>4986)
                     /locus_tag="VFB52_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07353.1"
                     /translation="ADTPLVLTCLVVGLSGVFRSIGLTGYNTMAFTDVPEEQMRDANT
                     LAVTTQQLSMGLGVAFAAVALRAGEPLGELLPGREEPAAAYSAAFLIVAVAALIACAG
                     ALRLHRSAGEVLRVGRRGAAAEEAVG"
BASE COUNT          751 a         1773 c         1760 g          702 t
ORIGIN      
        1 tcgtcggcag cgcgctcgcc gacgaggacg gcgtcacctg cgacatgacc ctgatgcgcg
       61 gggtcgagat cgacttcgac accaaggtgg tgcacgtgca gggcggctgc aagctcggcg
      121 acgtcgacga ggcgacggct gagcacgggc tcgccgtgcc ggccgggatc gtctccgaca
      181 ccggtgtgcc cgggctggcg ctcggcggcg ggatcggctg gctctcgcgc aagcacgggc
      241 tgacctgcga caacttcgtc tcgctggagg tggtgaccgc ggccggcgag acgatcgagg
      301 tctccgccga ctcccacccg gacctgttct gggcgctgcg cggcggcggc ggcaacttcg
      361 gcgtcgtcac ccgcttcagc ttccaggcct atgacttcgg cccgacgatg cgcttcggcg
      421 ccgcgctcta ccacgaggac gaggcgcggg cggcgctgcg cgagtacgcg cggatttatc
      481 cttctctgcc gaacgtcgtc ggctggcacg tggcgatgaa gcgcgagatg ccgccgctgc
      541 cgttcgtccc cgaggagctg gtcgggacgc cgctggtgat gctcttctgc atgtggctcg
      601 acgacccgga gtccgaggag ggcgtggcga tggtcgagcg gctgacctcg atcggcgacc
      661 cggccgtggt cggctcggcg gtgatcccgt tcgggctcgg gatgcagcgc ttcctcgacg
      721 ccgagttccc cgacggcctg cgcaactaca cgaaagaggg gcacctggag gagctctcgg
      781 agggcgcgat cgacgcgctg gtcgacttct gggcgcagag cctcaactcg aacacgacga
      841 tcgaaggcga gctggcgatc tacgggctcg gtggcgtcgc ccgcgaagtg ccggagatgg
      901 acgccgcctt ctccaaccgc gacagtctct ggtggatcaa ctgggccaac cactggcacg
      961 acgcggtcga cgacgagcgg aacatggaga tcatccgcgc ctcctaccgc tcgatcgagc
     1021 cctggctcgg caaaggcgtc tacgtcaaca tgctcaacgt ggacgagctc gacagggtgg
     1081 tcgaggccta cggaggtccc gagaagtacg cccggctcgg ccgggtgaag gcgacctatg
     1141 acccgaccaa cctgttccgg atgaaccaca acatcaagcc ggtcgacgcg tgaacggcga
     1201 gtcgctgccg acccgggtcg gcgtcgccaa gctcggcggt gggccggagc tgctcgccgc
     1261 ctacggcgac cgggtggtct cgctgaccgc ggcgctgggc tggaaccacg cctcgaccgc
     1321 cgagctgatc ggccgctggg acgagctctc gccgcggctg cgcgaaatcg gcgagtcggc
     1381 gcccgaggtc tcggtcgagg acgccgcctg ggagccgccg gtgatgccgg cgaagctgat
     1441 ctgcgtcggc gccaactaca ccgaccacgt cgaggagatg gagaaggccg gcgcgccgaa
     1501 agtcgaggcg accgcctacc cgttcagctt cctgaagccg ccgagcacgg cgctggccgg
     1561 cagcggggtc gaggtcgcaa tgcccggcta cgcgaccgaa ctcgactggg aggcggagct
     1621 ggcggtcgtg atcggccgcc cagagctggc cgcggacgac ccgctcgggg cgatcttcgg
     1681 ctactcgaac ctcaacgacc tctcgctgcg cgacttcgtc gcccccttcc cccacccgct
     1741 cgggctcgac gcggtgatcg gcaagggctg ggacggcgcg gcgccgatgg ggccctggat
     1801 cacgctcgcc agcgaggccg gtgacccgga gcacatgtcg atcgagaccc gcgtcaacgg
     1861 cgcggtcaag cagcagagct cgaccgagaa gatgatcttc acggtggccg agatggtcgc
     1921 ctactacgcc cgcgtgctga ccctcgaggt cggcgacgtg atcgccaccg gcacccctgc
     1981 cggcgtcggc gcgggcgccc aaccgcccga gttcctggcc ccgggcgaca cagtcgaggt
     2041 ggagatcggt gggctgggta cgctcagcac tccgatcgtc gccccgcgca gcgcggtgtc
     2101 gctggacatt caccaggtct gaacttgaat tgaacaggag gagttgaaga tggcaaaggt
     2161 cctttgcgtg ctctacgacg acccggtaga cggctacccg ccggcctacg cacgcgacga
     2221 catcccgaag atcgagggct accccgacgg gcagagcgcc ccggccccga acgggatcga
     2281 cttcaccccg ggcgagctgc tcggctgcgt ctccggtgag ctcggcctgc gcccctacct
     2341 ggaggcccag ggccacgagc tgatcgtcac ctccgacaag gacggccccg gctccgagtt
     2401 cgagaagcat ctggccgacg cagagatcgt gatctcccag ccgttttggc ccgcctacct
     2461 gaccgccgag cggatcgcca aggcgccgag cctgaagatc gcgatcaccg ccgggatcgg
     2521 ctctgaccac gtcgacctgc aggccgcgat ggacgccggg atcacggtcg ccgaggtcac
     2581 ctactgcaac tccaactcgg tcgccgagca cgtcgtgatg atgatcctcg gcctcgtccg
     2641 caactacatc ccctcctaca agatcgtcgt cgagggcggc tggaacatcg ccgacgcggt
     2701 atcgcgctcc tacgacgtcg agggcatgca ggtcgggacc gtcgccgccg ggcggatcgg
     2761 gctcggcgtg ctgcgccgca tggcgccctt cgacgtcggc ctgcactaca tggaccgcca
     2821 cccgcttcct cccgaggtgg agaaggagct gaacgcgacc caccacgaga ccgtcgagtc
     2881 cctcgtcgag gtctgcgacg tggtcacgat caactgcccg ctgcacccgg agaccgaaca
     2941 cctcttcgac gaggagatga tcgcccggat gaagcgcggc gcctacctga tcaacaccgc
     3001 ccgcggcaag atctgcgacc gcgacgcggt ggtcagggcg ctggagtcag gccagctggc
     3061 cggctacgcg ggtgacgtct ggttccccca gcccgcaccc caggatcacc cctggcggac
     3121 gatgccccac cacggcatga ccccgcacgt ctccggctca agcctctccg cccaggcacg
     3181 ctatgcggcc ggcacccgcg agatcctcga gtgctacttc gagggacgtc cccagcgcga
     3241 ggagtacctg atcgtcgacg ggggagggct cgccgggacg ggggcgcact cctactcggc
     3301 gggcaacgcg accgggggga gcgaggaggc ggcggagttc aagggctgag ggcgggagtg
     3361 tcgagttatg cggccatagg gctgcataac tcgacagccc ctcagccggc cctgcgctcg
     3421 actgcgtcga tcagggcggt gtggttctcg gcgccgcggc cttcggcgtg gagctgctcc
     3481 atcagctgct tgacgaccgg ggtcgcgggg agctcgacgc cgcggtcttt ggcctcggcg
     3541 agggcgatgg cgaggtcctt gaggtggtgt tcgaggcgga agccgggctc gtgctcgcgg
     3601 ctcagcaggg cggggccgcg ggcctcgagc atgcggccgc cgaccatgcc gccggcgaag
     3661 acgtcgagga tcgtctccgg gtcgaccccg gcggcggcgc cgagggtcag cgcctcgacc
     3721 agtccctcga ggatcagcgc gacggcgacc tggttgcagg ccttgaccac ctggccggcg
     3781 ccggccgggc cgacgtgggt caccgaggtg cccatcgctt cgaacagcgg cagcgctcgc
     3841 tcgaagtcct cgcgctcgcc gccgaccatg atcgtcagcg tgcccgcgcg agcgccgacg
     3901 tcgccgccgg aaaccggtgc gtcgagcacg ccgatcccgc gctcggcgcc ggcggcggcc
     3961 agcaggcgcg cctcggccgg ggcgatcgtg ctcatgtcga tcgccagggc gccctccggc
     4021 agggcggcga ggatcccctc ctccccggtc atcagcgcgt gcacggtcgg gccgtcgggc
     4081 agcatcgtga tcgcggcgtc cgccccgcgc gccgccgcgg cggcgtcggc cgcgccctcg
     4141 gcgcccgccg cctccagcgc cgcgaccggc tccgggctgc ggttggcgac gacgacatca
     4201 tggccggccg cgagcaggtt ctcggccatc ggccggccca tcaggccgag gccgacaaag
     4261 gcgacgcggc tcacgagccg gcctcccagg tcgcgtagaa ctcgcgctcg cgcgagtcga
     4321 gcaggtcgaa cccttgtccg accgcgagcc gctggaagtg ctcggaggcg ccgtgcgcgg
     4381 cgtaagcccc ctcgtccgcg tactgctcgt agagcaggaa gacgcgcggg tcctcggggt
     4441 cgcggtggat cacgtaggcg aggttgccgg gctcggcccg ggtcggctcg accatctgcg
     4501 cgagcacgcc cgcgaccgcc tcctcgtttc cctcctgcgc cgtccatttg gcgatcacca
     4561 cgtaggccat ccgcgcctcc tcctcgttcg gttcaggttc atccaacagc ctcctcggcc
     4621 gcggcgccac gccgcccgac ccgcagcacc tcgccggcgc tccggtgcag gcgcagcgcg
     4681 cccgcgcagg cgatcagcgc cgcgaccgcg acgatcaaga acgccgccga gtaggcggcg
     4741 gccggttcct cacgcccggg cagcagctcg ccgagcggct cgccggcccg cagcgctacc
     4801 gcagcgaagg cgacgccgag gcccatcgag agctgctgcg tggtgaccgc gagcgtgttg
     4861 gcgtcgcgca tctgctcctc cggcacgtcg gtgaaggcca tcgtgttgta gccggtcagg
     4921 ccgatcgagc ggaagacgcc gctgagcccg acgaccagac aggtcaggac cagaggcgtg
     4981 tcggcg
//
LOCUS       DASRIG010000496         7269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3333295, whole genome shotgun sequence.
ACCESSION   DASRIG010000496 DASRIG010000000
VERSION     DASRIG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333295"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFB52_13280"
     CDS             complement(<1..332)
                     /locus_tag="VFB52_13280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07354.1"
                     /translation="MAELPIPPLEMREAVGPTDPAAFDNPGGAPVFPDLPESAYRSVL
                     DFGCGCGRLARQLIQQQPQPQRYLGIDLHPVMVEWCAANLAPAAPQFEFRHHDVHYPT
                     WNPGEGKP"
     gene            complement(333..1088)
                     /locus_tag="VFB52_13285"
     CDS             complement(333..1088)
                     /locus_tag="VFB52_13285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07355.1"
                     /translation="MSGDFASARAAIDAVDPWYHRIEVMPGLTTPGVNDSQLVLRALD
                     LPADLSGLRVLDIGTRDGFFAFECERRGAEVTAVDYMPAEETGFPVARELLGSQVDFV
                     QENVYELSYEKYGGFDLVLFLGVLYHLRDPMLALDRIWDVCRGRLIVETQTIDDALLT
                     PEGAFARLTDIDPRLAAIPLMQFFPGAALNNDPTCVWAPNDAALRAMLEEVGFSVESQ
                     LRIGQRGLAFATWQDDPERRYWRRRDRAVGRLE"
     gene            1215..4865
                     /locus_tag="VFB52_13290"
     CDS             1215..4865
                     /locus_tag="VFB52_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO07356.1"
                     /translation="MLVVDDYEGCMDPAAEPFELIGLEEIGLPDAERMAAAYDVTELS
                     TAVKPWLLRHLLAREGVDAITYLDPDILVTDSLAAIDSLAAEHGTVLTPHFTAPLPRD
                     GRKPSEEDILIAGTYNLGFISLADVAATGELLDWWSERLERHCVVDPANGLFVDQRWI
                     DLVPGLWPGLHVLRDPGYNLAYWNLPTRRLEAAGEGYRVDGQPLRFFHFSGFDPTRPR
                     ALSKHQNRIEVASSPALSRICDEYAGALVAHGYEQTRSWSYGWDALPNGVRVDHAVRD
                     VYREASADGAVNGIFDPRGAKRFVSYLNDAPKGGGVSRYARALRDSREDLLRAFPDVD
                     GRDAAAFERWLVESAPSLGISPLLLPGGGEGETASPPVGEDASQNGVQSRLGVNLAGY
                     LSSELGVGEAARQVAAALAARELPVAKIDVPVEEDELQPALAGLGEERFPFDVNLICA
                     NADMLPAIAAAAPPALLERRHTAGLWFWEVEQFPARWRDSFAHLDEVWTASEFVAEAL
                     RPDAPVPVRTIRIPVTPAEPGDTERSALSMPEGFCFLFVFDFRSVLRRKNPLGVVEAF
                     ARAFPPGSGASLMLKSVGADACPQQAAELAAAAAGHPDVHLFDGVVSAAAKNAMIAGC
                     DCYVSLHRSEGLGLTMAEAMYFGKPVIATAYSGNLDFMSAENSFLVDYELQPIGSGAE
                     PYPPAARWAEPSLEQAAELMRTVFEDRELAARVGARAAADIRASHSPEAAGNVLEERL
                     EAIRRERVVERLHAPRSAPATTSAAPLPASPAAAAAAPPAIGDPASARGRLQYLMRFE
                     QPPVREEAGPLRRGFKRLYMRLLRPFAAHQRRIDLSLRDAVVELGADLAAAERRLAAA
                     DASAGEELRGALADLDRRLQGLDADVRGAYAAQAEQLEAQVGQMDAQGKRQEAQGEQL
                     AAQSEQLAAARSRLDELEPLSEQLERDRSFVDALRARVDRLAAETEGTLGDLREGAEE
                     AGRLARAGAAKPYMADERFATREHPALGSVLGFEARAANGLAPGYRGFEDLFRGPEQM
                     IRERQHVYLPLLGDGPVLDAGCGRGEFLDLLREAGIEGRGVDLDPGMVERCREKGHEV
                     EQGDLLETLAAAAPASLGTIFCAQVAEHLEPEQLQRLLALSAERLRPGGKLIVETVNP
                     HSAAALKAFWVDPTHRSPLFPETLLALCQLSGFAAADAFCPLGEGEWETDRLSEGEYA
                     VLATAPSANGDG"
     gene            complement(4862..6586)
                     /locus_tag="VFB52_13295"
     CDS             complement(4862..6586)
                     /locus_tag="VFB52_13295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZO07357.1"
                     /translation="MSRLPSSERARWTLVVAAGCLVMVAINVWWVATYRHGYPMDIDE
                     AGYTMIAINDHLGFENGGLSGWWDAIQSQTPHAPLLPALTSVVHLVSTGVMQGFLVLI
                     LFGALLTLAAFGIGERLAGPRLGALAALVVATSQGMFIFSREYIFALPTAALLSCAVY
                     ALLLSDGLRLRRWAIACGALLGLMLLARTMAIAFVPGVLVAALAAILTRGQGDLPKRF
                     LNLGLLVASGVLVAATWYWNNIDPVVDYLTDFGYGSQAQNYGAEHSLLSWDRWNTVAG
                     RMAGEDLLAPLAVLLFAALVALAVLVIRRLRASEDRGGTLLKLASTDAFAVLVVVLAG
                     YAALTSSRNGGNGFSFPVSMLVPPLAVVALRHYRRALIPAVALVGAIAAINLVAHLDL
                     SSDLSRSRSVLVPGVGQTDWINGEPHAVVQVRRQVPGPPERFVEADRAWLDVDAQLAQ
                     IFLEPTGPEDLRPVAVFGVRSPIVNSNTVALAALLNHREGIGLSQILAEPDDSVASYE
                     TQLGNPLLGETVDLITTSANPGDYEPAPTQSKVEAAARRQGYRQIREIPLPDGRELRL
                     WTKTPPAP"
     gene            complement(6567..>7269)
                     /locus_tag="VFB52_13300"
     CDS             complement(6567..>7269)
                     /locus_tag="VFB52_13300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO07358.1"
                     /translation="PLAGAERCHTLPYGLDLSPIEAARADFDRAAARAEAGIAEGAEL
                     IVCVGTIEPRKAQLLLTQAFEAIAARHPRARLALVGANEEVDSTTLAEYLAVSPLRER
                     IDLIGVTPDVQRWYAMADLFVCASDVESLPRTVLEAMAWETPVLATAVYGLPELIADG
                     ESGWLCEPRDLERLVAALDRVLAIPAEERRQVGRAGRALVEERHSLPRYGEQMGALIE
                     RAVAGGGPADVKAAE"
BASE COUNT         1031 a         2616 c         2627 g          995 t
ORIGIN      
        1 tcgggtttgc cctccccggg gttccaggtc gggtagtgga cgtcgtgatg gcggaactcg
       61 aactggggcg ccgccggcgc caggttcgcc gcgcaccatt cgaccatcac cgggtgcagg
      121 tcgatgccga ggtagcgctg gggttgcggc tgctgctgga tcagctgccg ggccagccgc
      181 ccgcagccgc agccgaagtc gagcaccgag cggtacgcgc tctccggcag gtcggggaag
      241 accggggcgc cgccggggtt gtcgaaggcg gcggggtcgg tcggcccgac ggcctcgcgc
      301 atttccagcg gtgggatggg cagctccgcc atctactcga gcctgcccac ggcgcggtcg
      361 cggcggcgcc agtagcggcg ctcagggtcg tcctgccagg tggcgaaggc gagcccgcgc
      421 tggccgatcc gcagctggga ctcgaccgag aagccgacct cttccagcat cgcccgcagg
      481 gcggcgtcgt tgggcgccca gacgcaggtc gggtcgttgt tcagcgcggc gccggggaag
      541 aactgcatca gcgggatcgc ggcgagccgc gggtcgatgt cggtcaggcg ggcgaacgct
      601 ccttcgggcg tcagcagggc gtcgtcgatc gtctgcgtct cgacgatcag gcggccccgg
      661 cagacgtccc agatccggtc gagcgccagc atcgggtcgc gcaggtggta gagcacgccg
      721 aggaagagca cgaggtcgaa gccgccgtac ttctcgtagg agagctcgta gacgttctcc
      781 tggacgaagt cgacctgcga cccgagcagc tcacgggcga ccgggaagcc ggtctcctcc
      841 gccggcatgt agtcgaccgc ggtcacctcg gcgccgcgcc gctcgcactc gaaggcgaag
      901 aacccgtccc tcgtacctat gtccaaaaca cgcaggccgc tgagatcggc gggtaggtcg
      961 agcgcccgca gcaccaattg cgagtcgttg actccaggag tggtcaaacc cggcatcacc
     1021 tcgatgcggt ggtaccaggg gtcgaccgcg tcgatcgccg cccgggcact ggcgaaatct
     1081 ccgctcatgc cgataactca cccagaggtg gtagctgctt gaacatctgc acgattatcg
     1141 ccaagaacta tgcatcccat gcacgggttc tggccgaatc tttcagtcgc gtccacccgg
     1201 acggcgcctg cgcggtgctc gtcgtcgacg actacgaggg ctgtatggac cctgcggccg
     1261 agccgttcga gctgatcggg ctggaggaga tcggtctgcc cgacgcggag cggatggcgg
     1321 ccgcctacga cgtcaccgag ctctccaccg cggtcaagcc ttggctgctg cgccacctgc
     1381 tcgcccgcga gggggtggac gcgatcacct acctcgaccc cgacatcctc gtcaccgatt
     1441 cgctcgccgc gatcgactcg ctcgccgccg agcacggcac cgtgctgacg ccccacttca
     1501 ccgcgccgct gccgcgcgac ggccgcaagc cctccgaaga ggacatcctg atcgccggca
     1561 cctacaacct cggcttcatc tcgctggccg atgtcgcggc gacgggcgag ctgctcgact
     1621 ggtggtcgga gcgcctcgag cgccactgcg tggtcgatcc cgccaacggc ctcttcgtcg
     1681 accagcgctg gatcgatctg gtccccggtc tctggccggg actgcacgtg ctccgcgacc
     1741 ccggctacaa cctcgcctac tggaacctgc cgacgcgccg gctggaggcc gccggcgagg
     1801 gctaccgggt cgacggccag ccgctgcgct tctttcactt cagcggcttc gacccgactc
     1861 ggccgcgggc cctgagcaag catcagaacc ggatcgaggt agcctcctcg ccggcgctca
     1921 gccggatctg cgacgagtac gccggggcgt tggtcgccca tggctacgag cagacgcgca
     1981 gctggtccta cggctgggac gcgctgccca acggcgtccg ggtcgaccac gctgtgcgcg
     2041 acgtctaccg cgaggccagc gccgacggcg cggtcaacgg catcttcgat ccgcgcggcg
     2101 ccaagcgctt cgtcagctac ctgaacgacg cgccgaaggg cggcggagtg agccgctacg
     2161 cgcgcgccct gcgcgactcg cgcgaggacc tgcttcgggc cttccccgac gtcgacggac
     2221 gcgacgccgc cgccttcgag cgctggctgg tcgagagcgc cccctcgctg gggatctcgc
     2281 cgctgctgct gcccggcggg ggcgagggcg agaccgcctc gccgcccgtc ggagaggatg
     2341 cgtcgcagaa cggcgtgcag agccggctcg gggtcaacct ggccggctac ctgagctccg
     2401 agctcggggt cggcgaggcc gcgcgccagg ttgcggccgc actggccgcg cgcgagctgc
     2461 cggtcgcgaa gatcgacgtt ccggtcgagg aggacgagct gcagcccgcc ctcgcggggc
     2521 tgggagagga gcgcttcccg ttcgacgtca acctgatctg cgccaacgcc gacatgctgc
     2581 cggcgatcgc cgccgccgcg cccccggcgc tgctcgagcg ccgtcacacc gccggtctct
     2641 ggttctggga ggtcgagcag ttcccggcgc gctggcgcga ttcgtttgcc cacctggacg
     2701 aagtctggac cgcttcggag ttcgttgccg aggcgctgcg cccggatgcg ccggtgccgg
     2761 tgcggacgat ccggattccg gtcacgcccg ccgagcccgg cgacaccgag cggtcggcgc
     2821 tctcgatgcc cgaggggttc tgcttcctct tcgtcttcga tttccgcagc gtcctccgcc
     2881 gcaagaaccc gctcggcgtg gtcgaggcct tcgcccgtgc cttcccgccc ggctcggggg
     2941 cctcgctgat gctgaagagc gtcggcgccg acgcctgccc gcagcaggcg gccgagctcg
     3001 ccgccgccgc ggcggggcac cccgacgtcc acctcttcga cggggtcgtc tcggcggcgg
     3061 ccaagaacgc gatgatcgcc ggctgcgact gctacgtctc cctgcaccgc tccgaggggc
     3121 tcggcctgac gatggccgag gcgatgtact tcggcaagcc ggtgatcgcc accgcctact
     3181 ccggcaacct cgacttcatg agcgccgaga acagcttcct ggtcgactac gagctgcagc
     3241 cgatcgggtc cggtgcggag ccctatccgc cggcggcgcg ctgggccgag ccctcgctgg
     3301 agcaggcggc cgagctgatg cggacggtct tcgaggaccg cgagctggcg gcccgggtcg
     3361 gcgcccgggc cgccgccgac atccgcgcca gccactcgcc cgaggccgcc ggcaacgtgc
     3421 tcgaggagcg gctcgaggcg atccgccggg agcgggtggt cgagcggctg cacgctcccc
     3481 gcagcgcacc cgcgactacg tctgctgcgc cgctgccggc ttcgcccgca gccgccgccg
     3541 cagcgccgcc ggcgatcggc gacccggcct cggctcgcgg ccggctccag tacctgatgc
     3601 gcttcgagca gccgccggtc cgcgaggagg cgggaccgct gcgccgcggc ttcaagcgcc
     3661 tctacatgcg cctgctgcgc cccttcgccg cgcaccagcg tcgcatcgac ctgagcctgc
     3721 gcgacgcggt ggtcgagctc ggcgccgatc tggccgcggc cgagcggcgg ttggccgccg
     3781 ccgacgcgag cgccggcgag gagctgcgcg gcgcgctcgc cgacctcgac cgccgcctgc
     3841 aggggctcga tgccgacgtg cgcggcgcct atgccgccca ggccgagcaa ctggaggccc
     3901 aggtcgggca gatggacgcc cagggcaagc ggcaggaggc ccagggcgag caactggcgg
     3961 cccagagcga gcagctggcc gcggcgcgct cccgcctgga cgagctcgag ccgctgagcg
     4021 agcagctcga gcgtgaccgc tcattcgtcg acgccctgcg cgcccgggtc gaccgcctgg
     4081 cggcggagac cgagggcacg ctcggcgacc tgcgcgaggg cgccgaggag gccgggcgcc
     4141 tggcccgcgc gggcgccgcc aagccctaca tggccgacga gcgcttcgcc acccgcgagc
     4201 accccgcgct gggcagcgtg ctcggcttcg aggcccgcgc ggccaacggc ctcgcgccgg
     4261 gctaccgcgg cttcgaggac ctcttccgcg gtcccgagca gatgatccgc gagcgccagc
     4321 acgtctacct gcccttgctg ggcgacgggc cggtgctcga cgccggctgc gggcgcggcg
     4381 agttcctcga cctgctgcgc gaggcgggga tcgagggccg cggcgtcgac ctcgaccccg
     4441 gcatggtcga gcgctgccgt gagaagggac acgaggtcga gcagggcgac ctgctcgaga
     4501 ccctcgccgc cgctgcgccc gcgtcgctgg ggacgatctt ctgcgcccag gttgccgagc
     4561 acctcgagcc cgagcagctg cagcgcctgc tggcgctgag cgccgagcgg ctgcgccccg
     4621 gcggcaagct gatcgtcgag accgttaacc cccacagcgc ggcggcgctg aaggcgttct
     4681 gggtcgaccc aacccaccgc agcccgctct tcccggagac gctgctagcg ctctgccagc
     4741 tctccggctt cgccgccgcc gacgccttct gcccgctcgg cgagggcgag tgggagaccg
     4801 accgcctgag cgagggcgag tacgcggtgc tcgccacggc gccgtctgcc aacggcgacg
     4861 gctagggcgc tggcggagtt ttcgtccaga ggcgcagctc gcggccgtca ggcaggggga
     4921 tctcgcggat ctgccggtag ccctggcggc gggcggcggc ttcgaccttg gactgggtcg
     4981 gggccggttc gtagtcgccc gggttggcgc tcgtcgtgat caggtcgacg gtttcgccga
     5041 gcagaggatt gccgagctgg gtttcgtagc tggcgacaga gtcgtcgggt tcggccagga
     5101 tctgactgag cccgatgcct tcgcgatggt tgagaagagc ggccagggcc accgtgttgg
     5161 agttgacgat cgggctgcgg accccgaaca cggcaacggg acgcaggtct tccggaccgg
     5221 tcggttcgag gaatatctgg gccagctggg catcgacgtc gagccaggcg cggtcggcct
     5281 cgacgaagcg ctccggcggg ccggggacct ggcggcgcac ctgcaccact gcgtgcggct
     5341 cgccgttgat ccagtcggtc tggccgactc cgggaaccag gaccgagcgc gagcgggaga
     5401 ggtcgctgga gagatccagg tgggcaacca ggttgatcgc ggcgattgcg ccgacgagcg
     5461 ccacggccgg gatcagcgcg cgccggtaat ggcgcagagc aaccaccgcg agcggcggta
     5521 ccagcatcga gaccgggaag gaaaagccgt tgccgccgtt gcgcgagctg gtcagcgctg
     5581 cgtagccggc gagcacgacc acgagcacgg cgaaggcgtc ggtggaagcc agcttcagca
     5641 gcgtcccgcc gcggtcctcg ctggcgcgaa ggcgacggat cacgagcacc gcgagcgcga
     5701 ccagggcggc gaagagcagg accgccagcg gcgcgagcag gtcttcgccg gccatccgtc
     5761 ccgccacggt gttccaccga tcccaggaga gcagcgaatg ctccgcgccg tagttctgcg
     5821 cctgcgaccc gtagccgaag tcggtcaggt agtcgacgac cgggtcgatg ttgttccagt
     5881 accaggtcgc cgcgaccagc accccgcttg cgaccaggag gccgaggttg aggaagcgct
     5941 tgggcaggtc tccctggccg cgggtgagga ttgccgccag cgccgccacc aggacccccg
     6001 gaacgaaggc gatcgccatc gtccgcgcca gcagcatcag cccgagcagc gcgccgcagg
     6061 cgattgccca gcgccgcagc cgcagcccgt cgctgagcag cagcgcgtag acggcgcagg
     6121 agagcagcgc cgcggttggc agggcgaaga tgtactcgcg gctgaagatg aacatgccct
     6181 ggctggtcgc gaccacgagc gccgccagcg cgccgagccg cggccccgct agccgctcgc
     6241 cgatcccgaa cgcggcgagg gtgagcagcg ccccgaacag gatcaggaca agaaagccct
     6301 gcatcacgcc ggtgctgacg aggtgcacca ccgaggtcag cgccggcagc agcggcgcgt
     6361 ggggggtctg gctctggatc gcgtcccacc agccgctgag cccgccgttc tcgaagccga
     6421 gatggtcgtt gatcgcgatc atcgtgtagc cggcctcgtc gatgtccatc gggtagccgt
     6481 ggcggtaggt ggcgacccac cagacgttga tcgcgaccat cacaaggcag ccggcggcga
     6541 ccaccagcgt ccagcgggcg cgctcgctac tcggcagcct tgacatcggc cggtcccccg
     6601 ccggcgacgg cacgctcgat cagcgcgccc atctgctctc cgtaacgcgg cagcgagtgc
     6661 cgctcttcca ccagggccct gccggcgcgg ccgacttgcc ggcgctcctc ggccgggatc
     6721 gcgagcacgc ggtcgagcgc cgcgaccagg cgctcgaggt cgcgcggctc acagagccag
     6781 ccgctctcgc catcggcgat cagctccggc agcccgtaga ccgcggtggc cagcacgggc
     6841 gtctcccagg ccatcgcctc gagcaccgtg cgcggcagcg actcgacgtc ggaggcgcag
     6901 acgaacaggt cggccattgc gtaccagcgc tgcacgtccg gggtgacccc gatcaggtcg
     6961 atccgctcgc gcagcggcga gaccgcgagg tattcggcga gcgtggtcga gtcgacctcc
     7021 tcgttggcgc cgaccagggc gaggcgagcg cgcgggtggc gcgcggcgat cgcctcgaag
     7081 gcctgggtca gcagcagctg cgccttgcgc ggctcgatcg tgccgacgca gacgatcagc
     7141 tcggcgccct cggcgattcc agcttcggct cgggcggcgg cgcggtcgaa atccgcccgg
     7201 gcggcctcga tcgggctcag gtcgagaccg tagggcagcg tgtggcagcg ctcggctccg
     7261 gccagcggc
//
LOCUS       DASRIG010000497         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3624117, whole genome shotgun sequence.
ACCESSION   DASRIG010000497 DASRIG010000000
VERSION     DASRIG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624117"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFB52_13305"
     CDS             complement(<1..641)
                     /locus_tag="VFB52_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZO07359.1"
                     /translation="MEALADLLAEARQRTLALVEPLDDEQRNRVYSPILSPLAWDLGH
                     IANFEELWLVQTVGGRAPLHGELGRFYDAIENPRKTRGELPILRDAELRAYMAEVRER
                     ALEVLAEVEVGPDVEDRLLRDAFVYEMLLAHELQHCETMLQLLQLVDGYELPLPIGPA
                     PVDGDGPEMVSVAAGTYEVGAGPRGFAYDNERDRHAVELAAFEIDRTPVTNAA"
     gene            751..1512
                     /locus_tag="VFB52_13310"
     CDS             751..1512
                     /locus_tag="VFB52_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07360.1"
                     /translation="MRIDGSGALVSGGASGLGAATARALATAGAKVVIADRDADLGKE
                     LAAEIGGDFLPVDVTEPDAVAAAVERAAAAPGGLRISVCCAGIGWAERIARSRGAHDL
                     DNFAKVIGVNLIGTFNVLRTAAAAMIENEPDEGGERGVCINTASIAAYDGQIGQAAYS
                     ASKAGIVGLTLPAARDLASKGVRVMTIAPGVFDTRLLEGLPEAARLSLGAEVPFPNRL
                     GSPAEYAQLVGQIVTNPMLNGETIRLDGALRMTPK"
     gene            1515..1925
                     /locus_tag="VFB52_13315"
     CDS             1515..1925
                     /locus_tag="VFB52_13315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZO07361.1"
                     /translation="MRGSELISEFLKHSPFVVHLGIELREIEDDRCLLALPFREEVVT
                     IGDMVHGGAVATLVDTAAMAASWSAIEFDGEPPRGTTVGLTTDFLAAARAQELLAEAR
                     VLRRGSSLCFCEVKVHGAEDGKLLASGLVTYKLG"
     gene            1966..>2316
                     /locus_tag="VFB52_13320"
     CDS             1966..>2316
                     /locus_tag="VFB52_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07362.1"
                     /translation="MRRRSARLATLVTLSVACCLALPGVAGALDFKIVNQSGKEPNEV
                     WVTVAAGGEFDVPGMTDNVPKKLSEIPGEELTIEKLVSGRIYIAYGAGVSEATTFDSP
                     TRFDWAELTVTPSSF"
BASE COUNT          348 a          796 c          827 g          345 t
ORIGIN      
        1 tacgcggcgt tggtgaccgg ggttcggtcg atctcgaagg cggccagctc gaccgcgtgg
       61 cggtcgcgct cgttgtcgta ggcgaagccg cgtgggccgg cgccgacctc ataggtgccg
      121 gcggcgacgc tgaccatctc gggaccgtca ccgtcaaccg gggcggggcc gatcggcaac
      181 ggcagctcgt aaccgtcgac cagctgcagc agttgcagca tcgtctcgca gtgctggagc
      241 tcgtgcgcga gcagcatctc gtagacaaat gcgtcgcgca ggaggcggtc ctcgacgtcg
      301 gggccgacct cgacctccgc cagcacctcg agcgcgcgct cgcgcacctc ggccatgtag
      361 gcgcgcagct cggcgtcgcg gaggatgggc agctcgccgc gggtcttacg cgggttctcg
      421 atcgcgtcgt agaagcggcc cagctctccg tgcagcggcg cgcggccgcc gaccgtctgc
      481 accagccaga gctcctcgaa gttggcgatg tggccgagat cccaggcgag cgggctgaga
      541 atcggcgagt agacgcggtt gcgctgctcg tcgtcgagcg gctcgacgag cgcgagcgtg
      601 cgctgtcgcg cctcggcgag caagtctgcg agcgcctcca ctccggctgg tttcgcctcg
      661 gtgtttgccg tcacctcggt agcgatacga agaccctacc caggtcggat taccggcatg
      721 tgaacacgca agcacgttag ggtgcgcgcg atgaggatcg acggaagcgg agcgctggtc
      781 agcggcgggg cctcgggcct cggcgccgcc accgcacggg cgctggcgac ggccggagcc
      841 aaggtcgtga tcgccgaccg cgacgccgac ctcggcaagg agctggccgc ggagatcggc
      901 ggcgacttcc tcccggtcga cgtgaccgag cccgacgcgg tcgcggccgc ggtcgagcgc
      961 gccgccgccg cgcccggcgg cctgcggatc tcggtctgct gtgccgggat cggctgggcc
     1021 gagcggatcg cccgctcgcg cggcgcccac gacctcgaca acttcgccaa ggtgatcggc
     1081 gtcaacctga tcggcacctt caacgtcctg cgcacagccg cggcggcgat gatcgagaac
     1141 gagcccgacg agggcggcga gcgcggcgtc tgcatcaaca cggcctcgat cgcggcctac
     1201 gacggtcaga tcggccaggc cgcctactcc gcctcgaagg ccgggatcgt cgggctgacg
     1261 ctgccggcgg cgcgcgacct cgcctcgaag ggggtgcggg tgatgacgat cgcgcccggc
     1321 gtcttcgaca cccgcctgct cgaagggctg ccggaggcgg cacggctctc gctcggcgcc
     1381 gaagtgccgt tcccgaaccg gctcggcagc ccggccgagt acgcgcagct ggtcggccag
     1441 atcgtcacca acccgatgct gaacggggag acgatccggc tcgacggcgc tctgcgcatg
     1501 accccgaagt agcgatgcgc gggtccgagc tgatctcgga gttcttgaag cattcgccgt
     1561 ttgtggtcca tctggggatc gagctgcgcg agatcgagga cgaccgctgc ctgcttgccc
     1621 tgcccttccg cgaggaggtg gtgacgatcg gcgacatggt ccacggcggc gcggtggcga
     1681 ccctggtcga caccgcggcg atggccgcct cctggtcggc gatcgagttc gacggcgaac
     1741 cgccgcgcgg gaccacggtc gggctgacca ccgacttcct cgcggcggcg cgcgcccagg
     1801 agctgctagc tgaagcgcgg gtgctgcggc gcggctccag cctctgcttc tgcgaggtca
     1861 aggtgcacgg cgccgaggac ggcaagcttc tcgccagcgg cctcgtcacc tacaagctcg
     1921 gctgatgcgt tcaagcagcg tttgccggct gccgatgggc gcggtatgag acgtcgatcc
     1981 gcacgcctgg ccacgctggt cacgctttcc gtcgcctgct gtctggccct gcccggcgtc
     2041 gccggcgcgc tcgacttcaa gatcgtcaac cagagcggca aagaaccgaa cgaagtctgg
     2101 gtcacggtcg ccgccggggg tgaattcgac gtgcccggga tgaccgacaa cgtccccaag
     2161 aagctgagcg aaatccccgg tgaagaactg acgatcgaaa aactcgtctc cgggcggatc
     2221 tacatcgcct acggggccgg ggtgagcgaa gcgaccacct tcgactcgcc gacccgcttc
     2281 gactgggccg agctgacggt gacgccgagc tccttc
//
LOCUS       DASRIG010000498         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_994711, whole genome shotgun sequence.
ACCESSION   DASRIG010000498 DASRIG010000000
VERSION     DASRIG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994711"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFB52_13325"
     CDS             <1..326
                     /locus_tag="VFB52_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZO07363.1"
                     /translation="VFAHEKLQEEGIRSRVVSLPCWEIFDRQDRSYRDEVLPPSVTAR
                     MAIEEASPLGWERYVGDAGEILAMRTFGQSAPFADVEAEFGFTPEKIAEAARKVAERA
                     ATPTT"
     gene            338..1441
                     /gene="tal"
                     /locus_tag="VFB52_13330"
     CDS             338..1441
                     /gene="tal"
                     /locus_tag="VFB52_13330"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZO07364.1"
                     /translation="MKATARLHELGQSLWLDNITRPMLAEGVLKGYIDELSVTGLTSN
                     PTIFDKAISAGDAYDQQIAEVSAAAEAGEGVGDNSDEKVFFELGVADLRDATNLFAGV
                     HERTDKVDGWCSLEVSPLLADQTQATIEQAALLHAKAERENLFIKIPGTQAGLPAIEE
                     SIFAGIPINVTLLFDDKHYLAAADAYMKGVERRIEAGLDPNVGSVASLFISRWDVAVA
                     ETVPAELKNRLGIAIGGRAFRAYHELLESDRMQRLLNEGARAQRLLWASTGTKDPDAS
                     DTLYIEALASPFTVNTMPEPTLVAFSQHGEVGELLPKDGGNAEAVLEEFANAGIDVAA
                     LAARLQDEGKVSFVKSWEDMLASIESKRGALTA"
     gene            1438..3075
                     /gene="pgi"
                     /locus_tag="VFB52_13335"
     CDS             1438..3075
                     /gene="pgi"
                     /locus_tag="VFB52_13335"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZO07365.1"
                     /translation="MSDGAPLRELPAWAALQSHYEGIKDTHLRELFAGDPQRGERLVA
                     HAAGLYLDFSKNRITDETVILLGELAKQRGVEARREAMFAGEHINVSEDRAVLHVALR
                     MPRNRSLVVDGVDVVKEVHETLDRMSAFAERIRSGEWVGHTGKPIRNVVNIGIGGSDL
                     GPVMAYEALRHYSRREMTFRFVSNIDGTDFAEATRDLDPEETLFIVSSKTFTTLETMT
                     NAHTARDWSLAGLGGEQAAVAKHFVAVSTNAEGVAEFGIDTDNMFGFWEWVGGRYSMD
                     SAIGLSTMLAIGPARFAEMLAGFHAVDEHFQEAPASSNLPMLMGLLSVWYGDFFGAET
                     NAVFPYDQYLHRFPAYLQQLTMESNGKHVTLDGTHVDYETGAIYWGEPGTNGQHSFYQ
                     LIHQGTRLIPCDFIGFANSLNPLGHHHDYLMSNVFAQGEALAFGKTPDEVRAEGTAEE
                     VVPHRVMEGNRPSNTILAERLTPETLGSLVALYEHSVFTQGAIWGIDSFDQWGVELGK
                     VLAKAIIPELESAEEPKLAHDSSTNALIRRYRSQRDA"
     gene            3135..>3698
                     /locus_tag="VFB52_13340"
     CDS             3135..>3698
                     /locus_tag="VFB52_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZO07366.1"
                     /translation="MPFLDALPLAASESNLLAAREMMAFTLAFHIVLSCLGVALPATI
                     LVANYLGLKRDDKDAMELARRWSKAMAVTFAVGAVTGTVLSFEFGLLWPEFMDRFGSV
                     FGVAFAIEGIFFFIEAIFLAIYIYGWKRLSPWAHFWSGVPMVVTGIGGAFSVVTANAW
                     MNQPQGFTLDAAGKVVDTDPLEVIFNPA"
BASE COUNT          611 a         1314 c         1211 g          562 t
ORIGIN      
        1 ccgtcttcgc ccacgagaag ctgcaggagg aagggatccg ctcgcgcgtc gtcagcctgc
       61 cctgttggga gatcttcgac cgccaggacc ggtcctaccg cgacgaggtg ctgccgccgt
      121 cggtcaccgc ccgcatggcg atcgaggagg cctcgccgct gggctgggag cgctacgttg
      181 gagatgccgg cgagatcctc gcgatgcgca cgttcggcca atcggcgccg tttgccgacg
      241 tcgaggcgga gtttggattc acgccggaga agatcgctga ggcagcgcgc aaggtcgccg
      301 agcgcgccgc cacgcccacg acctgaaggg ggtcgctgtg aaggcaaccg cacgactgca
      361 cgaactaggt cagagcctct ggctcgacaa catcacccgg ccgatgctcg ccgagggggt
      421 gctgaagggc tacatcgacg agctctcggt caccggcctg acctcgaacc cgacgatctt
      481 cgacaaggcg atctccgccg gcgatgccta tgaccagcag atcgccgagg tcagcgccgc
      541 cgccgaggcc ggcgaggggg tcggcgacaa ctccgacgag aaggtcttct tcgagctcgg
      601 ggtcgccgac ctgcgcgacg ccaccaacct cttcgccggg gtgcacgagc gcaccgacaa
      661 agtcgacggc tggtgctcgc tggaggtctc gccgctgctc gccgaccaga cccaggcgac
      721 gatcgagcag gcggcgctgc tgcacgcgaa ggccgagcgc gagaacctct tcatcaagat
      781 cccgggcacc caggcggggc tgccggcgat cgaggagtcg atcttcgccg ggattccgat
      841 caacgtcacc ctgctgttcg acgacaagca ctacctcgcc gccgccgacg cgtacatgaa
      901 gggcgtcgaa cggcggatcg aggccgggct cgaccccaac gtcggctcgg tcgcctcgct
      961 gttcatcagc cgctgggacg tcgccgtcgc cgagacggtg ccggccgagc tgaagaaccg
     1021 cctggggatc gcgatcggcg ggcgcgcctt ccgcgcctac cacgagctgc tcgagtccga
     1081 ccgcatgcag cgcctgctca acgagggcgc ccgcgcccag cggctgctct gggcgagcac
     1141 cgggaccaag gaccccgacg cctccgacac gctctacatc gaagcgctgg cctccccgtt
     1201 cacggtcaac acgatgccgg agccgaccct ggtcgccttc tcccagcacg gtgaggtcgg
     1261 tgagctgttg ccgaaagacg gcggcaacgc cgaggcggtt ctggaggagt tcgccaacgc
     1321 cggaatcgac gtcgccgcac tggccgcccg cctgcaggac gagggcaagg tgagcttcgt
     1381 caagtcctgg gaagacatgc tcgcctcgat cgagtccaag cgcggggcgc tcaccgcgtg
     1441 agcgacgggg cgccgctgcg ggagctgccc gcttgggcgg cgctgcagtc ccactacgag
     1501 gggatcaagg acacccacct gcgcgagctg ttcgcgggcg acccccagcg cggcgagcgc
     1561 ctggtcgccc acgccgccgg cctctacctc gacttctcga agaaccggat caccgacgag
     1621 acggtgatcc tgctcggcga gctggccaag cagcgcgggg tcgaggcgcg ccgcgaagcg
     1681 atgttcgcgg gcgagcacat caacgtctcc gaggaccgcg ctgtgctgca cgtggcgctg
     1741 cggatgccgc gcaaccgctc gctggtcgtc gacggcgtcg acgtggtcaa ggaggtgcac
     1801 gagacgctcg accggatgtc ggcgtttgcc gagcggatcc gctccggcga gtgggtgggc
     1861 cacaccggca agccgatccg caacgtcgtc aacatcggca tcggcggctc cgacctcggc
     1921 ccggtgatgg cctacgaggc gctccgccac tacagccgcc gcgagatgac ctttcgcttc
     1981 gtctccaaca tcgacggcac cgacttcgcc gaggccaccc gcgacctcga ccccgaggag
     2041 acgctgttca tcgtctcctc gaagaccttc acgacgctgg agacgatgac caacgcgcac
     2101 accgcccgcg actggtcgct ggccggcctg ggcggcgagc aggccgcggt cgccaagcac
     2161 ttcgtcgccg tctcgaccaa cgccgagggc gtcgccgagt tcgggatcga caccgacaac
     2221 atgttcggct tctgggagtg ggtcggcggc cggtactcga tggactcggc gatcgggctc
     2281 tcgaccatgc tggcgatcgg cccggcccgg ttcgccgaga tgctggccgg gttccacgcg
     2341 gtcgacgagc acttccagga agcgcccgcc agctccaatc tgccgatgct gatgggcctg
     2401 ctttcggtct ggtacgggga cttcttcggc gccgaaacca acgccgtctt cccctacgac
     2461 cagtacctgc accgcttccc cgcctacctg cagcagctga ccatggagtc gaacggcaag
     2521 cacgtgaccc tggacggcac ccacgtcgac tacgagaccg gggcgatcta ctggggggag
     2581 ccggggacca acggccagca cagcttctac cagctgatcc accagggcac gcgcctgatc
     2641 ccctgcgact tcatcggctt tgcgaactcg ctcaacccgc tcggccacca ccacgactac
     2701 ctgatgtcga acgtcttcgc ccagggcgaa gcgctggcct tcggcaaaac gccggatgag
     2761 gtccgagccg agggcaccgc cgaggaggtc gtcccgcacc gggtgatgga gggcaaccgg
     2821 ccctcgaaca cgatcctcgc cgagcgcctc accccggaga ccctcggctc gctggtcgcc
     2881 ctctacgagc acagcgtctt cacccagggc gcgatctggg ggatcgactc cttcgaccag
     2941 tggggggtcg agctgggcaa ggtgctggcc aaagcgatca tccccgagct tgagtccgcc
     3001 gaggagccaa agctcgccca cgacagctcg accaacgcgc tgatccggcg ctaccgctcc
     3061 cagcgcgacg cctagtcgtc caggcgtgag ccgcatcacc ctcagcgccc gaccgagggg
     3121 atagcctggc gttcatgcca ttcctcgacg cgctccccct ggcggcgtcg gagtccaatc
     3181 tccttgccgc acgggagatg atggcattca cgctcgcctt ccacatcgtc ctctcctgcc
     3241 tgggagtggc gctgccggcg acgatcctgg tcgccaacta cctcggcctc aaacgcgacg
     3301 acaaagacgc gatggagctg gcccggcgct ggtcgaaggc gatggcggtc accttcgcag
     3361 tcggcgccgt caccggcacc gtgctcagct tcgagttcgg gctgctctgg cccgagttca
     3421 tggaccgctt cggctccgtc ttcggggtcg ccttcgcgat cgaggggatc ttcttcttca
     3481 tcgaggcgat cttcctcgcg atctacatct acggctggaa gcggctctcg ccctgggccc
     3541 acttctggag tggggtgccg atggtcgtca ccgggatcgg cggagccttc tcggtggtca
     3601 ccgccaacgc ctggatgaac cagccacagg gcttcaccct cgacgcggcg gggaaggtgg
     3661 tggacaccga cccgctcgag gtcatcttca accccgcc
//
LOCUS       DASRIG010000499         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3625363, whole genome shotgun sequence.
ACCESSION   DASRIG010000499 DASRIG010000000
VERSION     DASRIG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625363"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFB52_13345"
     CDS             complement(<1..1448)
                     /locus_tag="VFB52_13345"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZO07367.1"
                     /translation="MAVSKKRKIIIATVVVLLLAAIVGASVFARRGDLPEVQTAKVER
                     RPQLESKVTANGEVRPIQFINLTAEVAGRVTDVYVKEGDQVRKGAPLLRVDPTQQGSA
                     TNIQEANLRAAQADVQTQNVAITAAENTINSARAALNTAQADLERAKFDRINAEIELK
                     RRTELVESGVESRSIYDTAKLRYDQANASVTSAEARVRQQEVAVRDAEIRVNQAKASL
                     AGSQARVAQSQAGLRREADLLSKTTQHASINGVIVGPLVQVGTFALANFQSTPLMIIA
                     DMSVINVEVRVDETDIATVAVGQRAKVKVDALGERELFGEVVEKAASAVTRSGQTIAQ
                     TAIAASQEAKDFKVVVRLVDMTDELRNRLRPGMSATATITTDRREGVMAIPMQALVER
                     DLNEGKPGASPAPSPSPGAEKKDRKPTKGVFLVQNNKAVFAPVETGITGENDIEITSG
                     LQEGQEIITGPYRQLRNLKNDAQIKREDKNKKP"
     gene            complement(1687..>2666)
                     /locus_tag="VFB52_13350"
     CDS             complement(1687..>2666)
                     /locus_tag="VFB52_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07368.1"
                     /translation="LAREDSAYEDVASKFMEHFVYISRAMNNIAGKGIELWDKRDGFY
                     YDVLHLPDGTTFPMRVRSLVGLIPLFAVETLESDVLDKLPGFKRRMQWFIDNLPEFSD
                     HIETITMENDLVRRNLSLVDRGRLRRLLGVLLDEAEFLSPFGVRSLSRFHREHPYRLH
                     VEGTEHRVDYEPAESSTHLFGGNSNWRGPVWFPVNFLIIESLQKFHYFLGDSFKVECP
                     TGSGRMMNLWDVAGELSRRLTLLFLKGADGRRPVYGGSEKFQTDPHWRDLILFYEYFH
                     GDNGAGIGASHQTGWTGLVAKLIQQAHRRTRASSTALLSTAGAAADRRG"
BASE COUNT          404 a          887 c          890 g          485 t
ORIGIN      
        1 ggcttcttgt tcttgtcctc gcgcttaatc tgggcgtcgt tcttaaggtt gcgcagctgg
       61 cggtaggggc cggtgatgat ctcctggccc tcctgcagcc cgctcgtgat ctcgatgtca
      121 ttctcgccgg tgatcccggt ctcgaccggc gcgaagacgg ccttgttgtt ctggacgagg
      181 aagacgccct tggtcggctt gcggtccttc ttctcagcgc ccggcgacgg agacggcgcg
      241 ggcgaggcgc ccggcttgcc ctcgttgagg tcgcgctcga ccagggcctg catcgggatc
      301 gccataacac cctcgcggcg gtcggtggtg atcgtcgcgg tcgccgacat ccccgggcgc
      361 agccggttgc gcagctcgtc ggtcatgtcg acaaggcgga cgacgacctt gaagtccttc
      421 gcctcctgcg aggcggcgat cgccgtctgc gcgatcgtct gccccgagcg agtcacggcc
      481 gaggcggcct tctcgacgac ctcgccgaag agctcgcgct cgccgagggc gtcgaccttg
      541 actttcgccc gctgcccgac ggcgaccgtc gcgatgtcgg tctcgtcgac gcgcacctcg
      601 acgttgatca ccgacatgtc ggcgatgatc atgagcgggg tcgactggaa gttggcgagg
      661 gcgaaggtcc cgacctgcac cagcgggccg acgatgacgc cgttgatcga cgcgtgctgc
      721 gtcgtcttgg agaggaggtc ggcctcgcgg cgcaggcccg cctgcgactg cgcgacgcgc
      781 gcctgcgagc cggccagcga ggccttggcc tggttgaccc ggatctcggc gtcgcgcacc
      841 gcgacctcct gctgccggac gcgcgcctcg gccgaggtga ccgaggcgtt ggcctggtcg
      901 tagcgcagct tggccgtgtc gtagatcgag cgggattcca ccccgctctc gaccagctcg
      961 gttctgcgct tgagctcgat ctcggcgttg atgcggtcga atttggcgcg ctcgaggtcg
     1021 gcctgcgccg tgttgagcgc cgcccgcgcg ctgttgatcg tgttctcggc ggccgtgatc
     1081 gcgacgttct gggtctgcac gtcggcctgc gccgcgcgca ggttagcctc ctggatgttg
     1141 gtcgccgagc cctgctgcgt cgggtcgacg cgcagcaacg gggcgccctt cctgacctga
     1201 tcgccttcct tgacgtagac gtcggtgacg cgcccggcga cctcggccgt aaggttgatg
     1261 aactggatcg gccggacctc gccgttggcc gtgactttcg actcgagctg cgggcgccgc
     1321 tcgaccttgg ccgtctgcac ctcggggagg tccccccgcc gcgcgaagac gctggccccg
     1381 acgatcgccg ccagcaagag gacgacgaca gtagcaataa tgatcttcct tttcttcgag
     1441 actgccatgg gcgcgcactg cctccaacca ggtaatgact gcgactggat ctcactttga
     1501 tataacgccg tcgccgcccc aacgttaacc gtatacgacg ccttttctcc cggtgtttca
     1561 agagatttac aaaggattaa aggccattgt aagtgcccgt ccggctgcag acaaggccct
     1621 ctcgcccatg aggcgcgcaa aacccgcggc gtcacagatt actaagttca aatccccgct
     1681 gcgcgctcac ccgcggcggt ccgccgccgc gccggcggtc gagaggaggg cggtcgagga
     1741 ggcccgcgtt cggcggtggg cctgctggat aagcttcgcc acgaggccgg tccagcccgt
     1801 ctggtgactc gccccgatcc ctgccccgtt gtccccgtgg aagtactcgt agaagaggat
     1861 caggtcccgc cagtgcgggt cggtctggaa cttctccgac ccgccataga ccggccgccg
     1921 cccgtccgcg cccttcagga aaagcagcgt cagccgccgc gagagctcac ccgccacgtc
     1981 ccagaggttc atcatccgac ccgagccggt cgggcactcg accttgaaac tgtcgccgag
     2041 gaagtagtgg aacttttgca gcgactcgat gatcaggaag ttgaccggga accagaccgg
     2101 cccgcgccag ttcgagttcc cgccgaagag gtgggtgctc gactcggctg gctcgtaatc
     2161 gacgcggtgc tcggtcccct cgacgtggag ccggtaaggg tgctcgcggt ggaagcgcga
     2221 gagcgagcgg acgccgaagg gcgagaggaa ttccgcctcg tcgaggagga cgccgaggag
     2281 gcgccgcagc cggccgcggt cgacgagcga gaggttgcgc cggacgaggt cgttctccat
     2341 ggtgatcgtc tcaatgtggt cgctgaactc cggcaggttg tcgatgaacc actgcatccg
     2401 ccgcttgaac ccggggagct tgtcgagcac gtccgactcc agcgtctcga ccgcgaagag
     2461 cgggatcagc ccgacgagcg agcgcacgcg catcgggaag gtggtcccgt cgggcaggtg
     2521 gagaacgtcg tagtagaacc cgtcccgctt gtcccagagc tcgatcccct tcccggcgat
     2581 gttgttcatg gcgcgcgaaa tgtagacgaa atgctccatg aacttcgagg ccacatcctc
     2641 gtaggccgag tcctcccgcg ccagct
//
LOCUS       DASRIG010000500         7749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2846780, whole genome shotgun sequence.
ACCESSION   DASRIG010000500 DASRIG010000000
VERSION     DASRIG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846780"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFB52_13355"
     CDS             complement(<1..1263)
                     /locus_tag="VFB52_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZO07369.1"
                     /translation="MAVFVCLALLGAALWGAGAARADKLITVNSTADSGAGLGCVGGE
                     TCTLRRAVEEITEGKYSGAVTVEFSVHGRIEIGAFGPLELEVPEKVTSVQIAGPGSGE
                     AVTVSGEEATRIFEIEGGEVTLERLTLAEGKRDGTTIGASGGGAIYQGGGTLLLRDML
                     LRDNSVESSRSGGAIYQDEGNLRIVDSTIEGSSTDSDVFAGSAGAIFVDTSNSSWLEL
                     DGTRITGSTAEGRGGAIFQNSGSMIIKDSELDHNTATNSGGALYADADGSEAKTTITE
                     SRLLANTSGSEGGAIYSDNGDAGLALVSSTVAGNKADGGAGIVVQGPTTVLRSTINAN
                     EAAATAGFHGGGIAVNSSTVEVVASTIANNVGEGIYFQFSGSSVVGSTIASNKSNGEA
                     GGIAGPEIISVTSSILAYNEGSSGPADCA"
     gene            complement(1350..2216)
                     /locus_tag="VFB52_13360"
     CDS             complement(1350..2216)
                     /locus_tag="VFB52_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZO07370.1"
                     /translation="MDSSLLTSVLLPAAIAVIMWSLGLTLTREDFRRVLTAPRGVAIG
                     MLNLALISPLLAVAMAELFNLPPELAVGLVLLGASPGGMMANMLTHLSRGDTALSVTM
                     TAISSVAAAITVPLFLELAGNHFGATELGDASMPGIVARVFAITIIPVAIGMEMRRRN
                     PAWTGAIYPRVRRISLGLFALVVLGAIASEHDTVTASLGEVAAAALALNLAAMGLSFT
                     ISRLARLDDRQATAIALELGIHNSALAIAVGATLSDEVAIPAAVYATFMLFTGGAFAW
                     TMRARNAEPASR"
     gene            complement(2276..3331)
                     /locus_tag="VFB52_13365"
     CDS             complement(2276..3331)
                     /locus_tag="VFB52_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZO07371.1"
                     /translation="MDAPGLEGIGSRTVAANGHRIYLLEAGEGPLVLFCHGFPEISYT
                     WRHQLRALADAGYHAVAVDMLGYGRSSKPAGVETRITEQIRTLVGVVAALGHDTACIV
                     GHDVGAPVAWSAAWTCPDVFDAVAGVSVPFGGRGLFAWPGDSFGAVRPSEHEGWIAGP
                     GKIHYQEYFSQPGALAREVEQDMRGWLNGLFYGTSARAPLPKVSDIPDADAALAFLRE
                     TAIVMTPGEGFADKLPTPGDDHWITAEELEVFVDSFERTGIEAALHSYRTLDLDWELL
                     GPYADVPLSVPAIFIAGEHDLPALWAVDAIERFPEVASRAFEPAILEGLGHWNCQEDP
                     EKFNAALLGFLAEAKSR"
     gene            complement(3348..4439)
                     /locus_tag="VFB52_13370"
     CDS             complement(3348..4439)
                     /locus_tag="VFB52_13370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07372.1"
                     /translation="MDLGIFMYPGHRPDQSLGDGAAWDLQMIRWADELGFSMVWIGEH
                     YTVPWETCPAPDLLIAQALMETDRVRLGAGSHNLPYYHPAALAHRVAYLDHLSKGRLN
                     FGAGASGTPTDLDLFGVDGNSGVNRKMMWESLDAIIRLWTEPGPYEWQGEFWTINKPK
                     TADGDILGFHIAPYQDPHPPISVSGVSPNSSTLKAAGERGFIPLSIAFNDAYLDGHWN
                     AYAAGAESAGLPAEREKWGIVREVFVAETDEEAVRLCLEGGVGDFLSGYWLPVVTRVG
                     LLDMYKHDPDVPDSEVTVDYILRNSAMVGSVDTVAEKIERSAEAVGGFGTLLLLGSDF
                     SSNPGAMRQSMELLANEVMPRVNSKLATA"
     gene            complement(4457..5734)
                     /locus_tag="VFB52_13375"
     CDS             complement(4457..5734)
                     /locus_tag="VFB52_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZO07373.1"
                     /translation="METVKDQAGRECPVFHSFDPLEHTEQLADPGLALEGARKDAPLF
                     FMEKYGLWCMTRYEDVREAFRDSETYSNASTIRVPDVPPEYADKLAHFPVKHAQPAGL
                     DAPEHTRIRKIAQQGFTQSMVRRFEPNMREFANNAIDKFIADGKADIVTEYAEVIPMQ
                     IAALMLGASVEDVPKLRQWNRDSIDLMIETLEGQRMFDLCERMIEFDRFMRDLIAERK
                     EQPTDDVLSLLVHAETDDGEPALSEGELIGLASVTLTAGTDTTGGLIGRMFYELLEDH
                     SRWDEVLADRELITPAVEETLRMYGIVHTLLRTTTREVEVAGVTLPAGTPVLLQLDSA
                     NHDAQRFEDSEKFDLHRPKAELADHLAFGKGTHFCIGAPFARPMAKIALKTMLERIPD
                     LALADAGAKPQVFPSLFVGGLTNLEAVWAPNKD"
     gene            5805..7022
                     /locus_tag="VFB52_13380"
     CDS             5805..7022
                     /locus_tag="VFB52_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZO07374.1"
                     /translation="MREPGSSEEGIGHRSAGLTGSFGSYARELYLRGHAGETPLIPAA
                     VDELERRAMAAMTPGAAAYIGAGAGSEDTMRANRAAFGRHRLVPRMMRDVAERELATA
                     VLGTAMPAPLLLAPIGAQSIVDPEGELASARAAAALGVPIVASSVSHFSLERIAEAAG
                     PDAPRWFQFYWPSDARLAASFVARAEAAGYTAIVLTVDTFVHGWKPRDLELAWLPFMD
                     GIGCGNYFEDPVFLEALERSPEEDQAAAVAHFFTVQGNPSITWENLEQLREMTSLPIL
                     LKGFMHPDDAREAARRGADGIVVSNHGGRQVDGAQAALDALPAIAEAVGEELTILFDS
                     GIRGGADALKALALGADAVCLGRPYIWGLTLAGEAGVDAVLRMTLAELDLTMALCGLT
                     AVDQIDARVLVDD"
     gene            complement(7008..>7749)
                     /locus_tag="VFB52_13385"
     CDS             complement(7008..>7749)
                     /locus_tag="VFB52_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07375.1"
                     /translation="ERPELIDKMTPAAPPLSSRPVICDADANVLHALLREDVELVDTP
                     IKRITASGIELVGGEQVPLDVIVFATGYKASDYLWPMEVEGREGVRLEELWSQDGPRA
                     YLGAMLPGFPNFFMLYGPNTNPYAGGAPVDYEEYVTRFALECFRGLIEGERRTVDVSA
                     DAYRRFGEEVDRWEAFKIYQAAGVRTYFQGRYGRSPVNGPIDMRKIWAWLRSPVAPID
                     GAAPIFAGGPVQEDAGLRPHFGADLIVD"
BASE COUNT         1095 a         2616 c         2813 g         1225 t
ORIGIN      
        1 cgcgcagtcg gcggggccgg agctgccctc gttgtaggcg aggatcgacg aggtgacgga
       61 gatgatctcc gggccggcga tcccgccagc ttcgccgttc gatttgttgg aggcgatggt
      121 cgagccgacg accgaggaac cgctgaactg gaagtagatg ccctcgccga cgttgttggc
      181 gatcgtcgag gccacgacct cgacagtgga gctgttcacg gcgatcccgc cgccgtggaa
      241 gccggccgtg gcagcggcct cgttggcgtt gatcgtgctc cgcagcaccg tcgtcgggcc
      301 ctggaccacg atcccggcgc cgccgtcggc tttgttgccg gcgaccgtcg aggagacgag
      361 agcgaggccg gcgtcgccgt tgtcggagta gatcgcgccg ccctcggagc cggaggtgtt
      421 ggcgagcagg cgcgattcgg tgatcgtcgt tttcgcctca gagccgtccg cgtcggcgta
      481 gagcgcgccg ccgctgttgg tcgcggtgtt gtggtcgagt tcgctgtctt tgatgatcat
      541 gctgccggag ttctggaaga tcgcgccgcc gcggccctcg gcggtcgagc cggtgatccg
      601 ggtgccgtcg agttcgagcc agctcgagtt gctggtgtcg acgaagatcg ccccggcact
      661 gccggcgaag acgtcgctgt cggtgctgct gccttcgatc gtcgagtcga cgatccgcag
      721 gttgccctcg tcctggtaga tcgcgccgcc gctgcgggag ctttcgaccg agttgtcgcg
      781 cagcagcatg tcgcgcagca gcagcgtgcc gccgccctgg taaatcgcgc cgccgccgct
      841 ggcgccgatc gtggtgccgt cgcgcttgcc ttcggccagg gtcagccgtt cgagcgtgac
      901 ttcgccgcct tcgatttcga agatcctggt cgcttcctcg ccgctgaccg tcaccgcttc
      961 gccgctgcca gggccggcga tctggaccga ggtgactttc tccggcactt ccagttcgag
     1021 cgggccgaag gcgccgatct cgatccgccc gtggacgctg aattcaaccg tgaccgcgcc
     1081 gctgtatttg ccctcggtga tttcctcgac ggcgcggcgc agggtgcagg tttccccgcc
     1141 cacgcagccg agcccggcgc ccgagtcggc cgtcgagttg acggtgatca gcttgtccgc
     1201 ccgagccgcg cccgcgcccc agagcgcagc gccaagtagc gccaggcaga cgaagacggc
     1261 caaccgtgcg agctttacgg tccggttcct cattgctccg gacctgatcg gcaacggcgc
     1321 gccccgaccc aaccccggag gggaaaattt cagcgcgagg ccggctcggc gttgcgggcg
     1381 cgcatcgtcc aggcgaaggc gccgccggtg aagagcatga aggtggcgta gaccgcggcc
     1441 gggatcgcga cctcgtcgga gagggtggcg ccgaccgcga tcgccagggc cgagttgtgg
     1501 atccccagct cgagcgcgat cgcggtcgcc tggcggtcgt cgagccgggc gagccgggag
     1561 atcgtgaagc tgaggcccat cgcggcgagg ttgagggcga gcgcggcggc ggcgacttcg
     1621 ccgaggctgg cggtgacggt gtcgtgctcg ctggcgatcg cgccgagcac gacgagcgcg
     1681 aagaggccga gcgagatgcg ccgcacccgc gggtagatcg ccccggtcca ggcggggttg
     1741 cggcgccgca tctccatccc gatcgcaacc gggatgatcg tgatcgcgaa gacccgggcg
     1801 acgatcccgg gcatgctcgc gtcgccgagc tcggtggcgc cgaagtggtt gccggcgagc
     1861 tcgaggaaga gcggcaccgt gatcgctgcc gcgacgctgg agatcgcggt catcgtcacc
     1921 gacagcgccg tgtcgccccg cgacaggtgc gtcagcatgt tcgccatcat cccgccgggc
     1981 gaggcgccga gcaggaccag cccgaccgcg agttcgggcg ggaggttgaa cagctccgcc
     2041 atcgccaccg ccaacaacgg tgagatcagc gccaggttga gcatcccgat cgcgaccccg
     2101 cgcggcgcgg tcagcacccg ccggaagtcc tcccgggtca gggtcagccc aaggctccac
     2161 atgatcaccg cgatcgcggc aggcagcaac accgaggtga gcagcgagct atccacgcgc
     2221 aggatgatcg ccgctccgcg gcgcggcgag cgttaacgca ctgctaggtc gagcttcagc
     2281 ggcttttggc ctcggcgagg aagccgagca gggcggcgtt gaacttctcc gggtcttcct
     2341 ggcagttcca gtggccgaga ccctcgagga tcgccggctc gaaggccctg ctggcgacct
     2401 cggggaagcg ctcgatcgcg tcgaccgccc agagcgccgg caggtcgtgc tcgccggcga
     2461 tgaagatcgc cgggaccgag agcgggacgt cggcgtaggg cccgagcagt tcccagtcga
     2521 ggtcgagggt gcggtaggag tgcagggcgg cctcgatccc ggtgcgctcg aagctgtcga
     2581 cgaaaacctc gagctcctcg gcggtgatcc agtggtcgtc gccgggggtc gggagcttgt
     2641 cggcaaaccc ttcgccgggg gtcatcacga tcgcggtttc gcgcaggaag gcgagcgccg
     2701 catcggcgtc gggaatgtcg gagaccttcg gcagcggcgc ccgcgccgag gtgccgtaga
     2761 agagcccgtt gagccagccg cgcatgtcct gctcgacctc gcgggcgagc gcgccgggct
     2821 gggagaagta ctcctggtag tggatcttgc ccgggccggc gatccagccc tcgtgctcgc
     2881 tcgggcgcac cgccccgaac gagtcgccgg gccaggcgaa gaggccgcgg ccgccgaagg
     2941 ggacgctgac cccggcgacc gcgtcgaaga cgtcggggca ggtccaggcg gcgctccagg
     3001 caaccggcgc gccgacgtcg tggccgacga tgcaggcggt gtcgtggccg agcgccgcga
     3061 ccaccccgac cagggtgcgg atctgctcgg tgatccgggt ctcgacccct gcgggcttcg
     3121 aggagcgccc gtagccgagc atgtcgacgg cgaccgcgtg gtagccggcg tcggcgaggg
     3181 cgcgcagctg gtggcgccag gtgtaggaga tctccgggaa gccgtggcag aagaggacca
     3241 gcggcccctc cccggcctcc agcaggtaga tccggtggcc gttggccgcg accgtgcgcg
     3301 acccgatgcc ctcgagccct ggggcgtcca tggctctcgc tccggggcta ggccgtggcc
     3361 agcttcgagt tgacccgcgg cattacctcg ttggcgagca gctccatcga ctgccgcatc
     3421 gcccccgggt tcgaggagaa gtcgctgccg agcaggagca gcgtgccaaa gccgccgacc
     3481 gcctccgccg agcgttcgat tttctccgcc acggtgtcga ccgagccgac catcgccgag
     3541 ttgcgcagga tgtagtcgac cgtgacctcg ctgtcgggga cgtcggggtc gtgcttgtac
     3601 atgtcgagca gcccgacccg ggtgacgacc ggcagccagt agccggaaag gaagtcaccg
     3661 acgccgccct cgaggcagag gcggaccgct tcctcgtcgg tttcggcgac gaagacttcg
     3721 cgcacgatcc cccacttctc gcgctcggcc gggagcccgg cggactcggc gccggccgcg
     3781 taggcgttcc agtggccgtc caggtaggcg tcgttgaagg cgatgctgag cgggatgaag
     3841 ccgcgttcgc cggccgcctt cagggtgctc gagttggggc tgacgccgct caccgagatc
     3901 ggcgggtgcg ggtcctggta gggggcgatg tggaagccga ggatgtcgcc gtcggcggtc
     3961 ttcggtttgt tgatcgtcca gaactcgccc tgccactcgt agggcccggg ctcggtccag
     4021 aggcggatga tcgcgtcgag cgactcccac atcatcttgc ggttcacgcc cgagttgccg
     4081 tcgacgccga agaggtcgag gtcggtcggg gtgccgctgg cgccagcgcc gaagttgagc
     4141 cggcctttcg agaggtggtc gaggtaggcg acccgatggg cgagcgcggc cgggtggtag
     4201 tagggcaggt tgtgggagcc ggcgccgagg cggacccggt cggtctccat cagcgcctgg
     4261 gcgatcagca ggtcgggggc ggggcaggtc tcccacggca ccgtgtagtg ctcaccgatc
     4321 cagaccatgc tgaagccgag ctcgtccgcc cagcggatca tctgcagatc ccaggcggcg
     4381 ccgtcgccga gcgactggtc cggccggtgg ccggggtaca tgaagatgcc aaggtccatc
     4441 gagactctcc tcctggtcag tctttgttcg gggcccagac ggcctcgagg ttggtcaggc
     4501 cgccgacgaa gagcgagggg aagacctgcg gtttggcccc ggcgtcggcg agcgccaggt
     4561 ccgggatgcg ctcgagcatc gtcttcagtg cgatcttcgc catcggccgc gcgaacgggg
     4621 cgccgatgca gaagtgcgtg cctttgccga aggccaggtg gtcggcgagt tcggctttcg
     4681 gccggtgcag gtcgaacttc tccgaatcct cgaagcgctg tgcgtcgtgg ttggcggagt
     4741 cgagctgcag cagcaccggc gtgcccgccg gcagggtcac cccggcgacc tcgacctcgc
     4801 gggtggtggt gcgcagcagg gtgtggacga tcccgtacat gcgcagcgtc tcctcgaccg
     4861 cgggggtgat cagctcgcgg tcggcgagca cctcgtccca gcggctgtgg tcctcgagca
     4921 gctcgtagaa catgcggccg atcaggccgc cggtggtgtc ggtgccggcg gtcagggtga
     4981 ccgaggcgag cccgatcagc tcgccctcgc tgagcgccgg ttcgccgtcg tcggtctcgg
     5041 cgtggaccag cagcgagagc acgtcgtcgg tcggctgctc tttgcgttcg gcgatcaggt
     5101 cgcgcatgaa gcggtcgaac tcgatcatcc gctcgcagag gtcgaacatc cgctgcccct
     5161 ccagcgtctc gatcatcagg tcgatcgagt cgcggttcca ctggcgcagc ttcggcacgt
     5221 cctcgaccga ggctccgagc atcaacgccg cgatctgcat cgggatcacc tcggcgtact
     5281 cggtgacgat gtcggccttg ccgtcggcga tgaacttgtc gatcgcgttg ttggcgaact
     5341 cgcgcatgtt cggttcgaag cggcgcacca tcgactgcgt gaagccctgc tgggcgatct
     5401 tgcggatgcg ggtgtgctcg ggcgcgtcga ggccggcggg ctgcgcgtgc ttgaccggga
     5461 agtgggcgag tttgtcggcg tactcgggcg gcacgtcggg gacccggatg gtcgaggcgt
     5521 tggagtaggt ctcgctgtcg cggaaggcct cgcgcacgtc ctcgtagcgg gtcatgcacc
     5581 agaggccgta tttctccatg aagaaaagtg gcgcgtcctt gcgcgctccc tccagcgcca
     5641 ggccggggtc ggccagctgc tcggtgtgct cgagcgggtc gaagctgtgg aagacggggc
     5701 actcccgtcc agcctggtcc ttcacggtct ccatcccacg tccctcctcg gtccctgaac
     5761 ccggatttcc ctccgggctg acagatttat atcatcttga tgatttgaga gagccgggaa
     5821 gctcagagga aggaatcggg catcgctccg ccgggctgac cgggagcttc ggcagctacg
     5881 cgcgtgagct ctacctgcgc gggcacgccg gtgaaacccc gctgatcccc gccgccgtcg
     5941 atgagctcga gcgccgcgcg atggcggcga tgacgccggg cgccgccgcc tacatcggcg
     6001 ccggggccgg cagcgaggac acgatgcggg ccaaccgcgc ggccttcggg cgccaccggc
     6061 tggtgccgcg gatgatgcgc gacgtcgccg agcgcgagct ggcgacggcg gtgctcggga
     6121 ccgcgatgcc ggcgccgctg ctgctcgccc cgatcggcgc ccagagcatc gtcgaccccg
     6181 agggcgagct ggccagcgcg cgggcggcgg cggcgcttgg cgtgccgatc gtcgcctcgt
     6241 cggtctcgca cttcagcctc gagcggatcg cggaggccgc cggtccggac gcgccgcgct
     6301 ggttccagtt ctactggccc agcgacgccc gcctcgccgc cagcttcgtc gcccgcgccg
     6361 aggcggccgg ctacacggcg atcgtgctca ccgtcgacac cttcgtccac ggttggaagc
     6421 cccgcgacct cgaactcgcc tggctgccgt tcatggacgg gatcggctgc ggcaactact
     6481 tcgaggaccc ggtcttcctg gaggcactgg agcgctcgcc cgaggaggac caggcggccg
     6541 cggtcgccca cttcttcacc gtccagggca acccctcgat cacctgggag aacctcgaac
     6601 agctgcgcga gatgacctcg ctgccgattc tgctgaaggg gttcatgcac cccgacgacg
     6661 cccgcgaggc ggcccgccgc ggcgccgacg ggatcgtcgt ctccaaccac ggcggccgcc
     6721 aggtcgacgg cgcccaggcg gcgctcgacg cgctgccggc gatcgccgag gcggtcggcg
     6781 aggagctgac gatcctcttc gacagcggca tccgcggcgg tgccgacgca ctcaaggccc
     6841 tcgccctcgg cgccgacgcc gtctgcctcg gccgtcccta tatatggggc ctgaccctgg
     6901 ccggcgaggc cggggtcgac gccgtcctgc ggatgacgct ggccgagctg gacctgacga
     6961 tggccctctg cggcctcacc gcggtcgacc agatcgacgc gcgggtccta gtcgacgatt
     7021 aggtcggcgc cgaagtgagg ccgcaggccg gcgtcctcct ggaccgggcc gccggcgaag
     7081 atcggcgcgg cgccgtcgat cggggcgacc gggctgcgca gccaggccca gatcttgcgc
     7141 atgtcgatcg ggccgttgac cggcgagcgg ccgtagcggc cctggaagta ggtccgcacc
     7201 ccggccgcct ggtagatctt gaacgcctcc cagcggtcga cttcctcgcc gaagcgccgg
     7261 taggcgtccg cgctgacgtc gaccgtgcgc cgctcgcctt cgatcaggcc gcggaagcat
     7321 tccagcgcga accgggtcac gtattcctcg tagtcgaccg gggcgccgcc cgcgtagggg
     7381 ttcgtgttcg gtccgtagag catgaagaag ttggggaacc cgggcagcat cgcgccgagg
     7441 taggcgcgcg gcccgtcctg cgaccacagc tcctcgagcc ggacgccctc acgcccctcc
     7501 acctccatcg gccagaggta gtcgctcgct ttgtaaccgg tcgcgaacac gatcacgtcg
     7561 agcggcacct gctcgccgcc gacgagctcg atcccgctcg cggtgatccg tttgatcggc
     7621 gtgtcgacca gctcgacgtc ctcgcgcagc agcgcgtgca ggacgttggc gtcggcgtcg
     7681 cagatcaccg gccgcgagga gagcggcggc gctgccgggg tcatcttgtc gatcagctcc
     7741 gggcgctcg
//
LOCUS       DASRIG010000501         7823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1538156, whole genome shotgun sequence.
ACCESSION   DASRIG010000501 DASRIG010000000
VERSION     DASRIG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538156"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFB52_13390"
     CDS             complement(<1..214)
                     /locus_tag="VFB52_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07376.1"
                     /translation="MNDEWRLQIDVQDDARATKIGEHLAARELEHDLSDAFEDRVIVS
                     RDGTRLFVYAGSREQVEAAGKLIQQVA"
     gene            complement(242..490)
                     /locus_tag="VFB52_13395"
     CDS             complement(242..490)
                     /locus_tag="VFB52_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07377.1"
                     /translation="MPTWIASKAAPAIWRRIPWKMVWTVSLWLAEKGRERVRENLTQS
                     EQTEFWGLIQKSKGRPSNLNQRDRTRMKKIAGKAIRGS"
     gene            complement(531..899)
                     /locus_tag="VFB52_13400"
     CDS             complement(531..899)
                     /locus_tag="VFB52_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZO07378.1"
                     /translation="MKLRALLVPALVALSLALASCGGGDDTDETTAIGGGNGTAATEV
                     EIVEFAYDPDPIEVEAGSTVSWTNEDSAPHTATADDGSFDTDTLDKGKTGKVTFEEAG
                     TFPYFCEIHPTMHGTVEVSE"
     gene            1031..3154
                     /locus_tag="VFB52_13405"
     CDS             1031..3154
                     /locus_tag="VFB52_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO07379.1"
                     /translation="MSRRLADAVTGPRGRWVAIAVWLAIGVAGWFGHDRIGEVTAAGQ
                     SSFLPSNSESTRAIEALETGGGNGAGAGEEVPTVIVFAREGGLTERDLTAIGRIGDGL
                     NRLEITGATPVIDPFSADASASLGKVADRAKGIGPLSRDGEAALVVLALDAGDRGAVT
                     KGVERLRQYLKVHAIPGVSSYVTGPGGIAADLETVAEEASETLLIATLSLVLILLLLV
                     YRAPVLALMPLLVVGAAYLVATGIAYLLIEAGWITVNSEGTMLLLVLIFGAGTDYSLL
                     LVHRYREEIEGGLHPSEALPKALSESLPAIAASAGTVIAAMLVLLVADLESTHWLGPI
                     LALGIATMLVASFTLLPALLAVLGTRAFWPRGQAKPARDPDRDGPGIWERVAGLVERR
                     ARVLILAIVIPLLVLGLGIKVDHGTIGFGQGETKPTNSSEGTEVLEEHFPPGLSSPLA
                     AVVATEKVERVTTGLERMPQTELTLPVPTEVDKSVVVIVLRGNPYSGEAAEDVEQIRE
                     RLHRIDPAGLLGGIPAENFDIEQTNERDTELIVPLVLVVIALILVAVLRAVVAPVFLI
                     ATVLLSFTATLGFCTFLFSELSSEGIAFNLELMAFLFLVALGVDYNIFLMTRAREEAA
                     TKGTRAGTLAALVHTGGVVTGAGLILAGTFATLTLLPLEELVQIGATVAIGVLLDTFV
                     VRALLIPSIAYRLDDRTWWPSKAKR"
     gene            complement(3159..4535)
                     /locus_tag="VFB52_13410"
     CDS             complement(3159..4535)
                     /locus_tag="VFB52_13410"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HZO07380.1"
                     /translation="MTEKSDPRHEAMLYGSRFLTEDAPDGPFPESGMSALDAMRLVDE
                     EMVLEGDPWRNLATFVTTWMEPEAQRIVAENAYRNFIDHAEYPISAEIEQRCIRMLAD
                     LYHAPGETTGCRTQGSSEAIMLGALSLKWKWKERREAESASVENPNLVFGGDVHVVWE
                     KFCRYFDVEPRIVPLQEGKYTIGPEDVQPHVDENTIGVAAVLGTTFTGHMDDIVGINK
                     FLVDLKGDKGLDVPLHVDGASGGFVWPFLYPDSEWDFRLEQVRSINVSGHKFGLVYPG
                     IGWLIFREKSDLAEDLVFYENYLGKTDATFTLNFSTGASMVLAQYYNLVRLGRQGYTY
                     VMKAMEMNAKALAARLKENGNFELIGADQEQLPLVAFKLKGDHDYDEFDVSWQLSAER
                     GWMLPAYTMPPNAEEVKVLRALVKQTLSREQVDRLADDIDQACKTLDEKGGAHPSERK
                     KTKVSPGY"
     gene            4682..5092
                     /locus_tag="VFB52_13415"
     CDS             4682..5092
                     /locus_tag="VFB52_13415"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HZO07381.1"
                     /translation="MGPIDYVLVEWPGRQPTGEALPHLVDLVDRGLIRIIDLAFITKG
                     EDGSVAMVEIADLGDEVAVFEGVSSGLLHDDDIEQAGEALEPGTSAALLVYENAWAAP
                     FAGAVRRSGGQLVASGRIPAEDFLAALDAAEATA"
     gene            5108..5398
                     /locus_tag="VFB52_13420"
     CDS             5108..5398
                     /locus_tag="VFB52_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO07382.1"
                     /translation="MPGLLRGVARTAVIAGTASAVGGRVHHRQEAKWAQQEEQQYQQQ
                     QAQQAPPPAAPAPAAPAGGESMIDQLKQLGELKDEGILTEEEFAAQKAKLLG"
     gene            complement(5418..5756)
                     /locus_tag="VFB52_13425"
     CDS             complement(5418..5756)
                     /locus_tag="VFB52_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07383.1"
                     /translation="MDSDRAKELLAREREQIEQAIANIKGAGAEEGDDRVEPGDFNSE
                     GLYQDEFDAGQLTDLNERLAAVERAEERLAKGTYGTSVRSGDPIPDERLEAFPTAELT
                     VEEAESGPRA"
     gene            5853..6920
                     /locus_tag="VFB52_13430"
     CDS             5853..6920
                     /locus_tag="VFB52_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07384.1"
                     /translation="MLAALAVALVAAAPAPAEQGEPTRDCVGESMLDGGNKRAIKFVV
                     WCSIESGKFSFELQRSDGGRENPVLPILAYTERPRVSGAGAGRASCRLGAEWLRCRGQ
                     KSAQVVVRGRIAVPVGTRCAVKVRIETEMARSAGRPAGCPGTRPQRPDFGRSSLRSYR
                     DLLGLLDGLDPEEIRRRIDASVRNWRRGEPVARVTAQEIGMPLLPVEQRQIVFRDELL
                     NNMDAIEAWFRARPTDTFAGYELVDGQRPVLYVGFTGDQVAQLAELKREVKLFAPNNI
                     EPFPVQPLYTESQLWDIGMKLVESDPQLERLIIEAGVATLENKVRIGTEHVAKVKRLL
                     AERFGPDGPFLVVFERGGTFL"
     gene            7045..>7823
                     /locus_tag="VFB52_13435"
     CDS             7045..>7823
                     /locus_tag="VFB52_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZO07385.1"
                     /translation="MTFRLIPLDDAIVFPTVTATLPIEVGEEERVFLIPRHDGEYGRV
                     GVVAEVIEHGRSRRGGPVATVVGLHRGLAGAAQPDENDPDVLRVEVQEIRDGHPDDEH
                     TVELAREYRAVVEEILELRGDDGRIAAFLRSVDEPGALADTSGLSPDINFEGKLRLLD
                     AVDVTQRLEIAVELQRERLAELQVRSKIRDDVESGAQKQQREYFLRKQMESIRKELGE
                     DETDLIAEYETKIDEAGMPEAVAEQAEKELRRLERQGEQSP"
BASE COUNT         1202 a         2710 c         2662 g         1249 t
ORIGIN      
        1 cggcgacctg ctggatcagc ttgccggcgg cctcgacctg ctcgcggctg ccggcgtaga
       61 cgaacagccg ggtgccgtcg cgggagacga tcacccggtc ttcgaaggcg tcgctgaggt
      121 catgttcgag ctcgcgcgcc gcgaggtgct cgccgatttt cgtcgcgcgg gcgtcgtcct
      181 ggacatcgat ctgcaaccgc cattcgtcgt tcatggcggc aaacatagtt gacccggccg
      241 cctaactacc ccgaatcgcc ttgccggcga tcttcttcat ccgcgtacgg tcccgctggt
      301 tgaggttcga ggggcgcccc ttcgacttct ggatcaggcc ccagaactcg gtctgctcgc
      361 tctgggtgag gttctcgcgc acccgctccc ggcctttctc ggccagccag agggagacgg
      421 tccaaaccat cttccagggg atccggcgcc agatcgcagg cgccgccttg ctggcgatcc
      481 aggtcggcat cagagccgag gttagaggac cgcgcgtccc gggcggcgcg ctattcggag
      541 acctcgacgg tgccgtgcat cgtcgggtgg atctcgcaga aataggggaa cgtcccggct
      601 tcctcgaacg tcaccttgcc ggtcttgcct ttgtcgagcg tgtcggtgtc gaagctgccg
      661 tcgtcggcgg tcgcggtgtg cggggcggag tcctcgttgg tccagctcac agtgctgccg
      721 gcttcgactt cgatcgggtc ggggtcgtag gcgaattcga cgatctccac ttcggtcgcg
      781 gcggtgccgt tgccgccccc gatcgcggtc gtctcgtcgg tgtcgtcgcc gccaccgcag
      841 gaggcgaggg ccagggagag ggcgaccagg gcggggacga gcagcgcgcg aagtttcata
      901 cgccaaagct acggccgcga gaggcggccg gatcaccgct ggccgggggc gactcggatt
      961 gccgtccgct gcccggggag ccgccccggc cgccactagc cttcggcccg tgtcgtcccc
     1021 cgagcccacc gtgagcaggc gactcgccga cgcggtgacg ggaccgcgcg gtcgctgggt
     1081 cgcgatcgcg gtctggctcg cgatcggcgt cgccggctgg ttcggccacg accggatcgg
     1141 cgaagtcacc gccgccggcc agagcagctt cctgccctcg aactcggaat cgacccgcgc
     1201 gatcgaggcg ctggagaccg gcggcggcaa cggcgccggg gcgggcgaag aggtgccgac
     1261 ggtgatcgtc ttcgcccgcg agggcgggct gaccgaacgc gacctgaccg cgatcggccg
     1321 gatcggcgac ggcctcaacc ggcttgaaat caccggcgcg accccggtca tcgacccctt
     1381 ctcggccgac gccagcgcct cgctcggcaa ggtcgccgac cgggcgaagg gcatcgggcc
     1441 gctctcccgc gacggcgaag cggccctggt cgtgctcgcc ctcgacgccg gcgaccgcgg
     1501 cgcggtcacc aagggcgtgg agcggctgcg ccagtacctg aaggtgcacg cgatccccgg
     1561 ggtcagctcg tacgtgaccg ggcccggcgg gatcgccgcc gatctcgaga cggtcgccga
     1621 agaagcgagc gaaacgctgc tgattgccac gctcagcctg gtcctgatcc tgctcctgct
     1681 cgtctaccgg gcgccagtgc tggcgctgat gccgctgctg gtcgtcggcg ccgcctacct
     1741 ggtcgcgacc ggaatcgcct acctgctgat cgaggcgggc tggatcacgg tcaactcgga
     1801 gggcacgatg ctgcttctgg tgctgatctt cggcgccggc accgactact cgctcttact
     1861 cgtgcaccgc tatcgcgagg agatcgaggg cggcctgcac ccgagcgagg cgctgccgaa
     1921 ggcgctgtcc gagagcctgc cggcgatcgc cgcctcggcc ggcaccgtga tcgcggcgat
     1981 gctggtgctg ctggtcgccg acctcgagtc gacccactgg ctcgggccga tcctggcgct
     2041 cgggatcgcg acgatgctgg tcgcctcctt caccctgttg ccggcgctgc tcgcggtcct
     2101 cggcacccgc gccttctggc cgcgcgggca ggcgaaaccg gcgcgcgacc ccgaccgcga
     2161 cgggccgggg atctgggaac gggtcgccgg gctggtcgaa cggcgggcgc gtgtcctgat
     2221 cctcgcgatc gtgatcccgt tactggtcct cgggctcggc atcaaggtcg accacggcac
     2281 gatcgggttc ggccagggcg agaccaagcc aaccaactcc agcgaaggca ccgaggtgct
     2341 ggaagaacac ttcccgccgg ggctgagctc gccgctggcc gcggtcgtcg cgacggagaa
     2401 ggtcgagcgc gtcacgaccg ggctcgaacg gatgccgcag accgaactga ccctgccggt
     2461 gccgaccgag gtcgacaagt ccgtggtcgt gatcgtgctg cgcggcaacc cctactcggg
     2521 cgaggcggcc gaagacgtcg agcagatccg cgaacgcctg caccggatcg accccgccgg
     2581 cctgctcggc ggcatcccgg ccgagaactt cgacatcgag cagaccaacg aacgcgacac
     2641 cgagctgatc gtgcccctgg tcctggtcgt gatcgcgctg atcctcgtcg ccgtcctgcg
     2701 ggcggtggtc gcgccggtct tcctgatcgc caccgtgttg ctctcgttca cggccacgct
     2761 cggcttctgc accttcctct tctccgagct ctccagcgaa ggcatcgcct tcaacctgga
     2821 gttgatggcg ttcctcttcc tggtcgccct cggggtcgac tacaacatct tcctgatgac
     2881 gcgggcccgc gaggaggcgg ccaccaaggg cacccgcgcc ggcactctcg ccgccctcgt
     2941 ccacaccggc ggcgtcgtca ccggcgccgg cctgatcctc gccggcacct tcgccaccct
     3001 caccctgctg ccgctcgagg agctggtcca gatcggcgcc acggtcgcaa tcggcgtcct
     3061 cctcgacacc ttcgtggtcc gcgccctcct gatcccctca atcgcctacc gcctcgacga
     3121 ccgcacctgg tggccgtcaa aagcaaagcg ctgacgcgtc agtagcccgg gctgaccttc
     3181 gttttcttgc gctcgctcgg atgggcgccg cccttctcgt cgagcgtctt gcatgcctgg
     3241 tcgatgtcgt cggcgaggcg gtcgacctgc tcgcggctga gcgtctgctt gaccagggcg
     3301 cgcaggacct tcacctcttc ggcgttcggg ggcatcgtgt aggcgggcag catccagccg
     3361 cgctcggccg agagctgcca ggagacgtcg aactcgtcgt agtcgtggtc ccccttcagc
     3421 ttgaaggcaa ccagcggcag ctgctcctgg tcggcgccga tcagctcgaa gttgccgttc
     3481 tccttcagcc gtgcggccag cgccttggcg ttcatctcca tcgccttcat cacataggtg
     3541 tagccctgac gtccgagacg gacgaggttg tagtactggg ccaacaccat cgaggcgccg
     3601 gtggagaagt tgagggtgaa cgtggcgtcg gttttgccga ggtagttctc gtagaagacg
     3661 aggtcctcgg ccaggtcgct cttctcgcgg aagatcagcc agccgatgcc ggggtagacg
     3721 agcccgaact tgtggccgga gacgttgatc gagcgcacct gctcgagccg gaagtcccac
     3781 tcggagtcgg ggtagaggaa gggccagacg aagcctcctg aggcgccgtc gacgtgcagc
     3841 ggcacgtcga ggcctttgtc gcccttgagg tcgaccagga acttgttgat gccgacgatg
     3901 tcgtccatgt ggccggtgaa ggtggtgccg agcacggcgg cgacgccgat cgtgttctcg
     3961 tcgacgtgcg gctgcacgtc ctcggggccg atcgtgtact tgccttcctg cagcggcacg
     4021 atccgcggct cgacgtcgaa gtagcggcag aacttctccc agaccacgtg cacgtcgccg
     4081 ccgaagacca ggttcgggtt ctcgaccgag gccgattcgg cttcgcgccg ctctttccat
     4141 ttccacttca gcgagagggc gccgagcatg atcgcctccg aggagccctg ggtgcggcag
     4201 ccggtcgtct cgccgggggc gtggtagaga tcggcgagca tgcggatgca gcgctgctcg
     4261 atctccgccg agatcggata ctcggcgtgg tcgatgaagt tgcggtaggc gttttcggcg
     4321 acgatccgct gcgcctccgg ctccatccag gtggtcacga aggtggcgag gttccgccag
     4381 gggtcgccct ccaacaccat ttcctcgtcg accaggcgca tcgcgtcgag cgccgacatc
     4441 cccgattccg ggaagggacc gtcgggagcg tcttcggtga ggaagcggct cccgtagagc
     4501 atcgcctcgt ggcgggggtc ggatttctcg gtcatggggc tccagtcttc gaaggcggga
     4561 ggcgagcgta tcccggacgg cggaggtcgg ccagcggtag gctcgcgacg atccccagat
     4621 acatcggaac gccttcccaa aaactgaaag gaccgccgtg aacggtgatg acttaattga
     4681 gatgggacca attgactacg tcttggtcga gtggcccggc cgccagccga ccggcgaggc
     4741 gctacctcac ctggtcgacc tcgtcgaccg tggcctgatc cggatcatcg acctcgcctt
     4801 catcaccaag ggcgaggacg gctcggtggc gatggtcgag atcgccgacc tcggcgacga
     4861 ggtcgccgtc ttcgagggcg tcagctccgg actgctgcac gacgacgaca tcgagcaggc
     4921 cggcgaggcg ctggagcccg ggacctcggc cgcgctgctg gtctacgaga acgcctgggc
     4981 cgccccgttc gccggagccg tgcgccgctc cggtggccag ttggtcgcca gcggccggat
     5041 ccccgccgaa gacttcctcg ccgcgctcga cgcggccgag gcaaccgcct aagagaagga
     5101 gaaaacgatg ccaggactac tcagaggcgt ggcccgcacc gccgtgatcg ccggaaccgc
     5161 gtccgccgtc ggcggccgcg tccaccaccg ccaggaagcg aaatgggcgc agcaggaaga
     5221 acagcagtac cagcagcaac aggctcagca ggcgccgccc cctgcggccc ccgctccggc
     5281 agctcccgcc ggtggggagt cgatgatcga ccagctcaaa cagctgggcg aactaaaaga
     5341 cgagggcatc ctcaccgagg aggagttcgc cgcccagaag gccaagctgc tcggctgaga
     5401 ctgcagcgcg ggtcgcgcta ggcgcgcggc ccgctctcgg cctcctcgac cgtcagctcg
     5461 gcggtcggga acgcctccag gcgctcgtcg gggatcgggt cgccgctgcg caccgaggtg
     5521 ccgtaggtgc ccttggcgag ccgctcctcg gcccgctcga ccgcggccag gcgctcgttc
     5581 aagtcggtca gctggccggc gtcgaactcg tcctggtaga gcccctcgga gttgaagtcg
     5641 cccggttcga cccggtcgtc gccctcctcg gcgcccgcgc ctttgatgtt cgcgatggcc
     5701 tgctcgatct gctcgcgctc acgcgccaag agctctttgg ctcggtctga atccatgccg
     5761 gaaccgtatc gatacacgcg tgtgacgcaa tcgcgtcaaa ctcgtagtca agggagacgt
     5821 cgatgaatcg tccacgaaca ctgctcgcaa cgatgctggc cgcgctcgcg gttgcgctcg
     5881 tcgctgcagc tcctgcgccc gcagagcagg gcgagcccac ccgcgactgc gtcggcgagt
     5941 cgatgctcga cggcggcaac aagcgtgcga tcaagtttgt cgtctggtgc tcgatcgaaa
     6001 gcggcaagtt cagcttcgag ctgcagcgca gcgacggcgg gcgcgagaac ccggtcctgc
     6061 cgatcctcgc ctacaccgag cgcccgaggg tgagcggcgc cggcgccggg cgtgcgagct
     6121 gccggctcgg cgccgagtgg ctgcgctgcc gcggccaaaa gtctgcacag gtcgtggtcc
     6181 gggggcggat cgccgtgccg gtcggaaccc gctgcgcggt caaggtgagg atcgaaaccg
     6241 agatggcgcg atccgccggg cgccccgccg gctgccccgg cacgcggccg cagcggccgg
     6301 acttcggccg cagctcgctg cgcagctatc gcgacctgct cgggctgctc gacggccttg
     6361 acccggagga gatccggcgc cggatcgacg cctcggtccg caactggcgc cgcggcgagc
     6421 cggtcgcccg ggtcaccgcc caggaaatcg gcatgcccct gctccccgtc gagcagcgac
     6481 aaatcgtctt ccgcgacgaa ctgctgaaca acatggacgc gatcgaagcc tggttccgag
     6541 cgcgtccaac cgacaccttc gccggctacg agctggtcga cggacagcgc ccggtcctct
     6601 acgtcggctt caccggcgac caggtggcgc agctggcaga gctcaagcgc gaggtgaagc
     6661 tgttcgcgcc aaacaacatc gagccgttcc ccgtgcagcc gctctacacc gaatcgcagc
     6721 tctgggacat tggtatgaag ctggtcgaat ccgacccgca gctcgagcgg ctgatcatcg
     6781 aagccggcgt cgcgacgctc gagaacaaag tccggatcgg gaccgaacac gtggccaagg
     6841 tgaaacggct gctggcggag cgattcggtc cggacggtcc cttcctggtc gtattcgaac
     6901 gcggcggcac cttcctctag cacgcggcgc gccggcgcgc cgccccctgc gccgtccgat
     6961 ccagagcgcg ccttcccgcc cttgagccga gggaggcatc gcgctatcct gatagaggac
     7021 taactacaga aagtggactt agcgatgact ttccgcctga tccctctaga tgacgcaatc
     7081 gtcttcccga ccgtgaccgc gaccctgccg atcgaggtcg gcgaggagga gcgcgtcttc
     7141 ctcattcccc gtcacgacgg cgagtacgga cgcgtcggcg tcgtcgccga ggtgatcgag
     7201 cacggccgct cgcgccgcgg cgggccggtc gcgaccgtcg ttggcctgca ccgcgggctc
     7261 gccggcgccg cccagcccga cgagaacgac cccgacgtct tgcgcgtcga ggtgcaggag
     7321 atccgcgacg gccacccgga tgacgagcac accgtcgagc tagcccgcga gtaccgcgcc
     7381 gtggtcgagg agatcctcga gctgcgcggc gacgatggcc ggatcgccgc cttcctgcgc
     7441 tcggtcgacg agcccggcgc cctggccgat acctccggcc tctcccccga catcaacttc
     7501 gagggcaagc tgcgactgct cgacgcggtc gacgtgaccc agcgcctgga gatcgcggtc
     7561 gagctgcaac gcgagcggct ggccgagctg caggtgcgct cgaaaatccg cgacgacgtc
     7621 gagtcgggcg cccagaaaca acagcgcgag tacttcctgc gcaaacagat ggagtcgatc
     7681 cgcaaagagc tgggcgagga cgagaccgac ctgatcgccg agtacgagac caagatcgac
     7741 gaggccggga tgccggaggc ggtcgccgag caggccgaga aggagctgcg ccggctcgag
     7801 cgccagggcg agcagtcgcc cga
//
LOCUS       DASRIG010000502         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3900953, whole genome shotgun sequence.
ACCESSION   DASRIG010000502 DASRIG010000000
VERSION     DASRIG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900953"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFB52_13440"
     CDS             <1..184
                     /locus_tag="VFB52_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07386.1"
                     /translation="PTGGIGWGPEMYSAKNGCSNQIEIGWKEGGSVNWKACMNPGGER
                     PSPGRFNYYRARYVAC"
     gene            417..>3284
                     /locus_tag="VFB52_13445"
     CDS             417..>3284
                     /locus_tag="VFB52_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZO07387.1"
                     /translation="MLALAAVVLVALGVIGIGVEKRLDPTTLEVPGTPASRANEMLRT
                     HFGDTAPFAILLEGPRVAIDRQGPRLVRALREDPAVTTLSPWDRGSVERLRPRPDRAL
                     ILADFHVDTRTAVNETVPHLNETLEREIHAPVTATQTGFASLSREIQEKSIEASERGE
                     LLALPFLLIVLLLVFRSPIAAAIPLAFGAITVVASRGILYFFTGWFDIDAFALAVCTM
                     MGLALGVDYALLMVSRFREELATGANPLDAARVTRRHAGRTVVFAGSTLVLSMVVSLF
                     ILPGSLLASLAGTLIMVVLLSIAVATIVGPALLVLIGPNVDRWRIGAAPDESSSRLMR
                     MVNGALKRPAPVAALIGAVLLVLAAPAIALKTGPPSPAQLPEDASARVDAEKIDDAIG
                     AGWDAPFQIVAVNEDGPITDANSLAALNRFQEGLAKLPGVQAVIGPEEATKRVKPLQQ
                     LGNAVLSSGGNIGPVKQLGRLGRELKTAAGGVSELREGIAEASDGAGLLALGSDRAGE
                     GAELIALGLGRAANGSQRAIDALDRFSDGSRKLEEALQEAAIGAQTIRSGILVSLAPI
                     LRRSSLGRLEEIGPSLESLGNEKVPSLIAPATVADEQLKAALAQLQAMTVGKTDPAYT
                     AALEAVVKASAAVSGTDPVTGQPYAEGYAGLPAELTAVQAALLEEVEEADEVVAFVDS
                     AIVEIERTGRGANELKVALYRMHKGANQLAFGAERLAKEATGLGEGLEQLSGGAAALV
                     GGLADLTDGAEALEAGLAEGVEEVAPLESGLDEASVRVLDGRARIRKQATRVADATPN
                     LFNSGYFVLSALDGAPPAAREQAASAIDLRRGGQAASVLVVSDYSFNTDGSKKLNAVL
                     DSEAQRLAEESGLEAGVAGGAAQLNDYGKVTKERIPWVMVGITLATFLVLVFVLRAIP
                     LAMIAVGLNLLTVGVAFGILTLLFEVPDDWPLGGRNYVDAVG"
BASE COUNT          530 a         1095 c         1191 g          468 t
ORIGIN      
        1 gccgaccggg gggatcggat ggggtcccga aatgtattcg gcaaaaaacg gctgcagcaa
       61 ccagatcgaa atcggctgga aagaaggtgg gtcagttaac tggaaggcgt gcatgaatcc
      121 cggcggtgaa cgacccagcc ccggaaggtt caactactac cgcgcgcgat acgtcgcctg
      181 ctagttcggc gctacgggct ggggtgaggg ccggccagac ggctgccctc acccaccggc
      241 cgagtttgga gctagttcgt ccaacgctta aataaagctt cacctcgatc cctgcgtcat
      301 ccagccacag ggaggaagat aatgaggtgg gttctaggag gagcagggta cggccagggc
      361 gctcgagctc ggcatcgtcc cagcggcact cggtgctgct gcggcatccg cgtgcggtgc
      421 ttgccctcgc ggcagtcgtg ctggtcgcgc tcggcgtcat cgggatcggc gtagagaagc
      481 ggctcgaccc gaccacgctt gaggttcccg ggacgcccgc ctcgcgcgcc aacgagatgc
      541 tgcgcaccca cttcggcgac accgccccgt tcgcgatcct gctcgaagga ccgcgggtgg
      601 cgatcgaccg ccagggcccg cggctggtgc gggccctgcg cgaagacccg gcggtgacga
      661 cgctctcgcc ctgggaccgc ggcagcgtcg aacggctgcg cccgcggccc gaccgggcgc
      721 tgatcttggc cgacttccac gtcgacacgc ggaccgcggt caacgagacc gtgccgcacc
      781 tcaacgagac gctcgaacgc gaaatccacg cgccggtgac cgcgacccag accggcttcg
      841 ccagcctctc gcgcgaaatc caggagaagt cgatcgaggc ctccgagcgc ggcgagctgc
      901 tggccctgcc gttcctgctg atcgtcctgc tgctggtctt ccgctcgccg atcgcggcgg
      961 cgatcccgct cgccttcggc gcgatcacgg tggtcgcctc gcgcgggatc ctctacttct
     1021 tcaccggttg gttcgacatc gacgcgttcg cgctcgccgt ctgcacgatg atgggcttag
     1081 cgctcggggt cgactacgcg ctgctgatgg tctcgcgctt ccgcgaggag ctggcgacgg
     1141 gggcaaaccc gcttgacgcg gcccgggtga cccggcgcca cgccgggcgc acggtcgtct
     1201 tcgccggcag cacgctggtg ctctcgatgg tcgtctcgct gttcatcctg ccggggtcgc
     1261 tgctggcctc gctggcgggc acgctgatca tggtcgtgct gctcagcatc gcggtggcga
     1321 cgatcgtcgg cccggcgctg ttggtcctga tcggcccgaa cgtcgaccgc tggcggatcg
     1381 gggcggcgcc ggacgagagc agctcgcggc tgatgcggat ggtcaacggc gcgctgaagc
     1441 gaccggctcc ggtcgcggcg ttgatcggcg cggtgctgct ggtgctggcg gcgccggcga
     1501 tcgcgctgaa gaccgggccg ccgagcccgg cacagctgcc cgaggacgcg tcggctcgcg
     1561 tcgacgccga aaagatcgac gacgcgatcg gggccggctg ggacgcgccc ttccagatcg
     1621 tcgccgtcaa cgaggacggg ccgatcaccg acgcgaacag cttggcggcg ctcaaccgct
     1681 tccaggaagg gctggcgaag ctgcccgggg tgcaggcggt gatcggcccc gaggaagcga
     1741 ccaaacgggt caaacccttg cagcagctcg gcaacgcggt gctctcctcg ggcggcaaca
     1801 tcggtccggt caaacagctc gggcggctgg ggcgcgaact caaaaccgca gcgggtgggg
     1861 tcagcgaact gcgcgaaggg atcgccgaag cgagcgatgg cgccggcctg ctggcgcttg
     1921 gctccgatcg cgccggcgag ggcgcggagc tgatcgcgct cgggctgggg cgggcggcga
     1981 acggcagcca gcgggcgatc gacgcgctcg accgcttctc ggatggctcg cgcaaactcg
     2041 aggaagcgct gcaggaagcg gcgatcggtg cgcagactat ccgcagcgga atcctggtca
     2101 gcctggcgcc gatcctgcgc aggagctcgc tcggacgact cgaagagatc ggcccctcgc
     2161 tggaaagcct tgggaacgaa aaggtgccga gcctgatcgc ccccgcgacc gtcgccgacg
     2221 aacagctgaa agcggcgctg gcccagctgc aggcgatgac ggtcggcaaa accgatcctg
     2281 cctacacggc ggcgctggaa gcggtggtga aagcgagcgc cgccgtcagc ggcaccgacc
     2341 cggtcaccgg gcagccctac gccgaaggct acgccggcct cccggccgaa ctgaccgcgg
     2401 tgcaggcggc gctgctcgaa gaagtcgaag aagccgacga agtcgtggcg ttcgtcgact
     2461 cggcgatcgt ggaaatcgaa agaaccggcc gcggcgccaa tgaactgaag gtcgccctct
     2521 accggatgca caagggcgcc aaccagctcg ccttcggcgc cgaacgcctg gccaaagagg
     2581 caacggggct gggcgaaggg ctggaacagc tgagcggcgg cgccgcggcg ctggtcggcg
     2641 gcctcgccga cctgaccgac ggtgccgagg cgctggaagc ggggctggcg gaaggggtcg
     2701 aagaagtggc gccgctggaa agcgggctcg acgaagcgag cgtgcgggtg ctggacggca
     2761 gggcgcggat ccgcaaacag gccacgcggg tcgccgatgc gacgccgaac ctgttcaact
     2821 ccggctactt cgtgctctcg gccctggacg gcgcgccgcc ggcggcgcgc gagcaggccg
     2881 cgagcgcgat cgacctgcgc cgcggcggcc aggcagcgtc ggtgctggtc gtctccgact
     2941 acagcttcaa caccgacggc tccaaaaaac tgaacgcggt gctcgactcc gaagcgcagc
     3001 ggctggccga ggaatccggc ctggaagccg gcgtggcggg cggcgccgcg cagctgaacg
     3061 actacggcaa ggtgacgaaa gagcggatcc cctgggtgat ggtggggatc acgctggcga
     3121 ccttcctggt gctcgtcttc gtgctgcggg cgatcccgct ggcgatgatc gcggtcgggc
     3181 tcaacctgct caccgtcggc gtcgccttcg gcatcctcac gctgctcttc gaggtgcccg
     3241 acgactggcc gctgggcggc cgcaactacg tcgacgcggt cggt
//
LOCUS       DASRIG010000503         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3626677, whole genome shotgun sequence.
ACCESSION   DASRIG010000503 DASRIG010000000
VERSION     DASRIG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3626677"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFB52_13450"
     CDS             complement(<1..582)
                     /locus_tag="VFB52_13450"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZO07388.1"
                     /translation="MARNLLDGTEGVRAGDEALSAMSTAALVGTMVADLDAVQESLAA
                     AAPAVTAAIDDVAARMSRGGRLIYVGAGTSGRLAQLDAVECPPTFGTNPERVQALLAG
                     GSGASKLAVEAAEDDEEDAVNQIAAVHPGPEDTVLGLSASGRTPFVLAAMREARRGGA
                     LTIGVSCNSSASLSLVVDHPIELIVGEELLSGST"
     gene            complement(625..1554)
                     /locus_tag="VFB52_13455"
     CDS             complement(625..1554)
                     /locus_tag="VFB52_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZO07389.1"
                     /translation="MAPAMTSPPDPADGGGPDGNDVSGDLLVRVRSVMPELRPSQRRV
                     AEAIVERPAWVAESSIETVAAGCATSTTTISRLVRALGFANYREFRAALSRAAAIEAG
                     TRPSEGPSGDIDRDDSLADLVTKIGHNETQAVAETARNLDLEQLEAAVAAVAGARRIE
                     IAGVGASALVGSDLRQKLHRIGLAASVWTEAHAAATAASLLGVSDVLIAISHSGKTAE
                     LLTAVEIAGQGGARTIAITNFARSPLARRAEIVLTTAAREATFRSGATTSRIAQLVVV
                     DALFVGVAVSSFDASTESLRLTREAVEELRRDH"
     gene            1716..2054
                     /locus_tag="VFB52_13460"
     CDS             1716..2054
                     /locus_tag="VFB52_13460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZO07390.1"
                     /translation="MHGSKAVRLSGDFSLVYTSGLVAARDTGGRGPIESQVHSVLGQL
                     RDLVAAAGGGMEDVVKLMAWLPRREDVPIYAEIRREYFGTASPASTSVISQLVEEDIL
                     IEVEAIVAVV"
     gene            2085..>3590
                     /locus_tag="VFB52_13465"
     CDS             2085..>3590
                     /locus_tag="VFB52_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HZO07391.1"
                     /translation="MERLTTHPLDPLNAEEIRGAAGIVKAIPDSTPSWRIVSVLPHEP
                     YKAELREWEGGGVRPPRRALVIVRDKELRRTIELVVDIGTSAVVSRTEPSGIQPAVMR
                     EESVEAERLAREHPDWQAAMRARGVTELERAMLDHMPLGYQDADDGPEVRRGIALTWL
                     RDGTGSDPSDWEGDNGYARPVEGLIVHFDLDEMKVLRVEDHGGAPIPEAAGNFTEAAL
                     LDPANVPSYPEGPRTSARPLEIAQPDGPGFSVDGWRVDWEKWQLRVGFNAREGLVLHD
                     VGYLDRGRRRPILHRASLSEMFVPYGDPAVTHFRKQLFDQGEAGIGRCVNSLRLGCDC
                     LGEIYYFDAVIHDNDGEPIELPNAICMHEEDYGLLWKHTTLRTGQPQSRRSRRLVISS
                     FTTVGNYDYGFFWYLYQDGRIEFEAKLTGILSTGAYAPGEEPRYGTPIAPGLYGPNHQ
                     HWFNVRLDMAVDGDANTLLEVNSRPVEDEETNFFGGAWTAEATVLGSELQAQ"
BASE COUNT          562 a         1215 c         1284 g          529 t
ORIGIN      
        1 ggtggagccc gagagcagct cctcgccgac gatcagctcg atcgggtgat cgaccacgag
       61 gctgagcgac gccgacgagt tgcacgacac gccgatcgtc agcgcgccgc cgcggcgcgc
      121 ctcgcgcatc gccgcgagca caaagggggt ccgcccgctg gcgctgaggc cgaggaccgt
      181 gtcctcggga ccggggtgga cggcggcgat ctggttgacg gcatcctcct cgtcgtcctc
      241 ggcggcctcg accgcgagct tcgaggcccc gctgccgccg gccagcagcg cctgcacacg
      301 ctccgggttg gtgccgaagg tcggtgggca ctcgacggcg tcgagctggg cgaggcggcc
      361 ggaggtgccg gcgccgacgt agatcaggcg cccgccgcgc gacatgcgag cggcgacgtc
      421 gtcgatcgcg gcggtgaccg ccggtgccgc ggcggcaaga gactcctgta cggcgtcgag
      481 atcggcgacc atcgtcccca ccagcgcggc ggtgctcatc gcactcaggg cttcgtcgcc
      541 cgcccgaacc ccctcggtcc cgtctagcaa gtttcttgcc atcagggcgg actataagca
      601 attaagttgc caaaccgaaa ccgctcagtg gtcgcggcgg agctcctcga ccgcctcacg
      661 agtcagtcgc agagactcgg tcgaggcgtc gaaggagctg accgcgacgc cgacgaacag
      721 cgcgtcgacg acgaccagct gggcgatcct gctggtcgtc gcacctgagc ggaaggttgc
      781 ctcgcgggcg gccgtcgtca gcacgatctc cgccctccgg gccagggggg accgggcgaa
      841 gttggtgatt gcgatcgtcc gcgcgccgcc ttggccggcg atctccactg cggtcagcaa
      901 ctcggcggtc ttaccgctgt gggagatcgc gatcaggaca tccgagaccc cgagcaggct
      961 ggcggcggtg gccgcggcgt gtgcctcggt ccagaccgag gcagcgagcc cgatccggtg
     1021 cagcttctgg cgcaggtccg aaccgaccag ggcgctcgcc ccgaccccgg cgatctcgat
     1081 ccgccgcgcc cctgcaaccg cggcgacggc cgcctcgagc tgctccaggt cgaggttgcg
     1141 agcggtctcc gcgaccgcct gcgtctcgtt gtggccaatc ttggtcacca ggtcggccag
     1201 cgagtcatca cggtcgatgt cgccactcgg gccctcgctt ggcctcgtgc cggcctcgat
     1261 cgccgccgcc cgggaaagcg cggcgcggaa ctcccggtag ttggcgaacc cgagtgcccg
     1321 gacgaggcgg gagatcgtcg tcgtgctcgt cgcacacccc gccgcgaccg tctcgatcga
     1381 ggactcggcg acccaggcgg ggcgctcgac gatcgcctcc gccacgcggc gctggctggg
     1441 gcggagctcg ggcatcaccg atctgacgcg gaccaggagg tcccccgaaa catcgttccc
     1501 gtcaggtcct ccgccgtctg cggggtcggg gggagaggtc atagctgggg ccatactctg
     1561 gcagacgcat cgggccagat ggaaggcctg ccggtccagg ctgtgcaatg gtctagtcca
     1621 ataggagcga cccccgagga aaggaccttc cgttgttcga catctctgcc gtcagcgatc
     1681 cctccgccca gccgttctcg ctaggaggta aggctgtgca cgggtccaag gcggtgcggc
     1741 tcagcggcga cttctccctc gtctacacct ccggcctcgt tgcggcgagg gacacgggcg
     1801 ggcgcgggcc gatcgagtcg caggtgcact cggtgctcgg ccagctgcgc gacctggtcg
     1861 cagcggccgg cggcgggatg gaggacgtgg tcaagctgat ggcctggctt ccgcgtcgcg
     1921 aggacgtgcc catctacgcg gagatccgcc gtgagtactt cggcaccgcc agtccagcca
     1981 gcacgtcggt gatatcgcag ctcgtcgagg aggacatcct gatcgaggtc gaggcgatcg
     2041 tcgcagtcgt ctaacattgg tctagaccaa tggagagagg agcaatggag agattgacca
     2101 cccacccgct tgaccccctg aacgcggagg agatccgcgg ggccgccggg atcgtcaagg
     2161 cgatcccgga cagcacgccg agctggcgca tcgtctcggt gctgccgcac gagccgtaca
     2221 aggcggagct gcgcgagtgg gagggcggcg gggttcgccc gccgcgccgg gcgctggtaa
     2281 tcgtgcgcga caaggagctc cgtcgcacga tcgagctggt cgtcgacatc ggtacctcgg
     2341 cggtcgtctc caggacggaa ccgagcggca tccagccggc ggtgatgcgg gaggagtcgg
     2401 tggaggccga gcgcctcgct cgtgagcatc cggactggca ggcggcgatg cgagcgcgcg
     2461 gggtgaccga gctcgagcgg gcgatgctcg accacatgcc actcggctac caggacgccg
     2521 acgacggccc cgaggtccgg cgggggatcg cgcttacctg gctgcgcgac gggactggtt
     2581 ccgacccatc cgactgggag ggcgacaacg gctacgcccg gccggtcgag gggctgatcg
     2641 tccacttcga cctcgacgag atgaaagtcc tgcgggtcga ggaccatggg ggcgcaccga
     2701 ttccggaggc cgcggggaac ttcaccgagg cggctctgct cgacccggcc aacgtgccca
     2761 gctaccccga gggcccgcgg acgagcgcgc gcccgctcga gattgcgcag ccggacggcc
     2821 ccggtttcag cgtcgacggc tggcgcgtgg actgggagaa gtggcagctg cgggtgggtt
     2881 tcaatgcccg cgaggggctg gtgctccatg acgtcggcta tctcgaccgc gggcggcggc
     2941 ggccgatcct gcaccgggcc tcgctctcgg agatgttcgt gccgtacgga gaccctgcgg
     3001 tgacccactt ccgcaagcag ctcttcgacc agggtgaggc cgggatcggg cgctgcgtca
     3061 acagcctccg gcttggttgc gactgcctcg gcgagatcta ttacttcgac gccgtgatcc
     3121 acgacaacga cggcgagccg atcgagctgc ccaacgcgat ctgcatgcac gaggaggact
     3181 acgggctgct ctggaagcac acgacgctga ggaccgggca gccgcaatcg cgccgcagtc
     3241 gccggctggt gatctcctcg ttcaccaccg tcggcaacta cgactacggg ttcttctggt
     3301 acctctacca ggacggtcgc atcgagttcg aggcgaagct gaccgggatc ctctcgaccg
     3361 gcgcctatgc gcccggggag gagccgcggt acgggacccc gatcgcgccc gggctgtacg
     3421 ggcccaacca ccagcactgg ttcaacgtca ggttggacat ggccgtcgac ggggacgcga
     3481 ataccctgct cgaggtgaac agccggccgg tcgaggacga ggagaccaac ttcttcggcg
     3541 gcgcctggac ggccgaggca accgtgctcg gcagcgagtt gcaggcccaa
//
LOCUS       DASRIG010000504         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1284548, whole genome shotgun sequence.
ACCESSION   DASRIG010000504 DASRIG010000000
VERSION     DASRIG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284548"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..524
                     /locus_tag="VFB52_13470"
     CDS             48..524
                     /locus_tag="VFB52_13470"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HZO07392.1"
                     /translation="MTDEQGVQPGGWPAPPPPPVAIPADWYPDPDAPETQLRYWDGNR
                     WTDHFSQVETASEAQAQVDPGWYVDPKDPATKRYWDGSQWSEWTDRNYSSKALGGKEN
                     PNNDTLAVVGWITAVIIPIVGLVIGIVLNTRDDQRGTWIMVTSAVFIALGLLIVAV"
     gene            complement(585..1421)
                     /locus_tag="VFB52_13475"
     CDS             complement(585..1421)
                     /locus_tag="VFB52_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZO07393.1"
                     /translation="MGRLPALVHRRELLGGNVQERGLSAYLAELIGSFLLVFFITSVV
                     TLYVSTGSQAQFGSDFAVVGFVQGLTLFGVIVMFGAVSGGHFNPAVTLAAATIRKISP
                     LDAVIYILAQLSGAVLGALLTKGLLEDEGRAVNYGAGEVSGLLSGNFAGAVVEGIGVF
                     ALTLVILAAVFSKKSVKDWAPLAIGTTFVFMVMVGGPLTGACFNPARWFGAALVSNEW
                     GGVWPYLVGPIVGALLAVGIWRFIIEGSGGAAPTEPPQRVKGEAPKAGAKKPPPPPPK
                     PA"
     gene            1453..2418
                     /locus_tag="VFB52_13480"
     CDS             1453..2418
                     /locus_tag="VFB52_13480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZO07394.1"
                     /translation="MKRAVALLAAVLVLAGVAGCGEDGAEPGAPQGASMVLDFTPNAV
                     HAGIFTALAKDFYKDGGVDLKVQAPGESTDAPKLLAAGRVEFAILDIHDLGIARERGL
                     DLVGVMPIVNTPLASIIARGDGPVKRPRDLVGRTIGVTGLPSDDAVVDSEVSADGGDP
                     AAVKRVTIGFNAVATLAAGKVDAATGFWNAEGVALRQQGVPVRIFKLGEYGAPGYPEL
                     VLTTSRATLDRDPDLVNSVVKATAGGYALAVEDPAAALEALLAANPSLDRADQDAQLR
                     ALGSDLRPAPFDPRVIEEWARWDLSHGLLTESVDIGAAFRLPGQS"
     gene            complement(2409..2732)
                     /locus_tag="VFB52_13485"
     CDS             complement(2409..2732)
                     /locus_tag="VFB52_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07395.1"
                     /translation="MAQVVSIAEDLMLASRVDVMLTGAGHHVILARSLEGASLDGAEL
                     IVADLEVADPEKLAATGVPVIGYYSHVDAEKKKAADAAGIDLAVPRSRMARELPALAE
                     RLLQD"
     gene            complement(2744..3106)
                     /locus_tag="VFB52_13490"
     CDS             complement(2744..3106)
                     /locus_tag="VFB52_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07396.1"
                     /translation="MALATDFQAILDTLPPDWTDLEVDLRIDDERQYIDSAVSLSQVN
                     AQVYSRAEWHWRLIVAKSFGHAAAPQTVLGVLAKLDGEGVTGELGLAEVREGRAEVVQ
                     MWGRPESVREEFRQRRSI"
     gene            complement(3121..>3484)
                     /locus_tag="VFB52_13495"
     CDS             complement(3121..>3484)
                     /locus_tag="VFB52_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07397.1"
                     /translation="RGLGGDFGIEDARPGERIGRSWVLAFGLIPFDYDDLTLERVDPG
                     RGFLERSTMLTQRLWEHERTIEPAGETCMLVDRVAWEPRLPVPGAWLAPLIAAIFRHR
                     HARLRRRFGGAPAAAPGE"
BASE COUNT          554 a         1250 c         1174 g          506 t
ORIGIN      
        1 cggcgcaccc tggcgaggct atcgaacggc ttgtacgatc ccccgccatg accgacgagc
       61 agggggttca accggggggc tggccagctc cgccgccacc gccggtggcg attccggcgg
      121 actggtatcc ggatccggac gcgcccgaga cccagctgcg ctactgggac ggcaaccgct
      181 ggaccgacca cttctcacag gtcgaaaccg cgtccgaggc acaggcgcag gtggatccgg
      241 gctggtacgt ggacccgaag gacccggcta cgaagcgcta ctgggacggg tcgcaatggt
      301 cggagtggac cgatcgcaat tacagctcga aagcgcttgg cggcaaggaa aacccgaaca
      361 acgacacgct cgccgtggtc ggctggatca ccgccgtgat catcccgatc gtcggcctcg
      421 tgatcgggat cgtgctcaac acccgcgacg accagcgcgg cacctggatc atggtcacct
      481 cggcggtctt catcgcgctc ggcctcctga tcgtcgcggt ctaaccccag aacgaaatcg
      541 acccggcgct tggccgggtc gatccgaaag gtgcttgttg ccgatcaggc gggcttcggc
      601 ggcgggggcg gcggtttttt ggcgccggct ttcggcgctt cgcctttgac ccgctgcggc
      661 ggctcggtcg gggcagctcc gccgctgccc tcgatgatga agcgccagat cccgacggcg
      721 agcagggcgc cgacgatcgg gccgacgagg tagggccaga cgccgcccca ctcgttgctg
      781 accagcgcgg cgccgaacca gcgggccggg ttgaagcagg ccccggtcag cggaccgccg
      841 accatgacca tgaagacgaa cgtggtgccg atcgccagcg gcgcccagtc cttgacgctc
      901 ttcttcgaga agaccgcggc gaggatcacc agggtgagcg cgaagacgcc gatgccctcg
      961 accaccgcac cggcaaagtt gccgctgagc aggccgctta cttcgccggc gccgtagttg
     1021 acggcgcggc cctcgtcctc cagcagaccc ttggtcagca gcgcgccgag cacggcgccg
     1081 gagagctggg cgaggatgta gatcaccgcg tccagcgggc tgatcttgcg gatcgtcgcc
     1141 gcggcgagcg tcacggccgg gttgaaatgt ccgccgctga ccgcaccgaa catcacgatc
     1201 acgccgaaga gggtgagtcc ctggacgaag ccgacgacag caaagtcgga gccgaactgg
     1261 gcctgactgc cagtcgacac gtaaagcgtg acgacactgg tgatgaagaa gaccagcaaa
     1321 aagctgccga tcaactcggc tagatatgcc gaaaggcctc gttcctgcac gtttcctcct
     1381 agtagctccc gtcggtgaac aagcgcgggc agtctaccca catttgctca atgaattagt
     1441 ttccagcgcc ggatgaaacg ggctgtcgcg ctgctggcgg ccgtcctcgt acttgccggc
     1501 gtcgccggct gcggcgagga tggcgcggaa ccgggcgcgc cgcaaggcgc gagcatggtg
     1561 cttgatttca ccccgaacgc cgtccatgcc gggatcttca ccgcgctggc taaagatttc
     1621 tacaaggacg gcggcgtcga cctgaaggtg caggcgccgg gcgaatcgac cgacgcgccg
     1681 aagctgcttg ccgccggtcg ggtcgagttc gcgatcctcg acatccacga cctcggcatc
     1741 gcccgcgagc gcggcctcga cctggtcggg gtgatgccga tcgtcaacac cccgctggcc
     1801 tcgatcatcg cccgcggcga cgggccggtg aagcgcccgc gcgacctcgt gggccgcacg
     1861 atcggcgtca ccggcctgcc ttccgacgac gccgtggtcg actccgaggt gagcgccgac
     1921 ggcggcgacc cggctgcggt gaagcgggtg acgattggtt tcaacgcagt cgccaccctc
     1981 gccgccggca aagtcgacgc cgcgaccggc ttctggaacg ccgagggcgt ggcgctgcgc
     2041 cagcagggcg tcccggtacg gatcttcaag ctcggcgagt acggcgcccc cggctacccg
     2101 gagctggtcc tgaccacctc gcgcgcgacg ctcgaccgcg acccggatct ggtcaactcc
     2161 gtggtcaagg cgaccgcagg cggctacgcg ctcgccgtcg aggaccccgc cgccgccctc
     2221 gaagccctgc tcgccgccaa ccccagcctc gaccgggccg accaggacgc ccagctccgc
     2281 gccctcggct ccgacctccg gccggccccc ttcgacccgc gcgtgatcga agaatgggcg
     2341 cgatgggatc tatcccacgg cctcctcacg gagtccgtgg acatcggcgc cgccttccgc
     2401 ctcccaggtc agtcctgaag cagacgctcc gcgagtgccg gtagttcgcg cgccatccgc
     2461 gacctaggca ccgcgagatc aatccccgcc gcgtccgccg ccttcttctt ctcggcgtcg
     2521 acgtgcgagt agtagccgat caccggcacc ccggtcgcgg ccagcttctc cggatcggcg
     2581 acttcgaggt cggcgacgat cagctcggct ccgtcgagcg aggccccctc cagcgagcgg
     2641 gcgaggatca cgtggtggcc ggcgccggtc agcatcacgt cgacgcggct cgccagcatc
     2701 aggtcctcgg cgatgctgac cacctgcgcc atcgcagacc ccgttagatc gagcggcgct
     2761 ggcggaactc ttcgcgcacg ctttcggggc ggccccacat ctgcacgacc tcggcccgtc
     2821 cctcgcgcac ctcggccagc cccagctctc cggtgacgcc ttcgccgtcg agcttggcca
     2881 gcaccccgag cacggtctgc ggcgcggccg cgtggccgaa gctcttggcg acgatcagcc
     2941 gccagtgcca ctcggcccgc gagtagacct gcgcgttgac ctggctcagc gagacggccg
     3001 agtcgatgta ctggcgctcg tcgtcgatcc gcaggtcgac ctcgaggtcg gtccagtcgg
     3061 gcggcagcgt gtcgaggatc gcttggaagt cggtggcgag ggccatgggg gaactgaagc
     3121 ctactcgccg ggcgcggcgg cgggcgcgcc tccgaagcgg cggcgcaggc gcgcgtgccg
     3181 gtggcggaag atcgccgcga tcagcggcgc cagccaggcg ccgggcaccg gcagccgcgg
     3241 ctcccaggcg acccggtcga ccagcatgca ggtctcccca gccggctcga tcgtgcgctc
     3301 gtgttcccag agtcgctggg tcagcatcgt cgagcgctcg aggaagccgc gcccgggatc
     3361 gacccgctcc agcgtgaggt cgtcgtagtc gaacgggatc aggccgaagg cgagcaccca
     3421 gctgcgcccg atccgctcgc ccggccgcgc gtcttcgatc ccgaagtcgc cgccaagccc
     3481 gcgc
//
LOCUS       DASRIG010000505         4706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_469691, whole genome shotgun sequence.
ACCESSION   DASRIG010000505 DASRIG010000000
VERSION     DASRIG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469691"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFB52_13500"
     CDS             complement(<1..347)
                     /locus_tag="VFB52_13500"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZO07398.1"
                     /translation="MPETAAALERLTYAKAVNAALDRALESFPEALLFGEDIAIPGGV
                     FGCTRGLREKHGERVFDTPISETGFLGAALGAAMVGRRPIVEVMWAEFTLVAFDQIVN
                     QAPNVRYVSEGRL"
     gene            complement(340..1290)
                     /locus_tag="VFB52_13505"
     CDS             complement(340..1290)
                     /locus_tag="VFB52_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZO07399.1"
                     /translation="MDGTRLLGFYEQMLRIRGTEEAIEDLREDGQVVGSVHLSCGQEA
                     VPVGVVAERSPQDPVFGNYRGHGWALACGAPPENVLAELAARALGVSGGRGGSAFFSA
                     PEWGFYGENAIIGAQVPMAVGAALAARYDDSDRVAITVFGDGAANQGAVSEAFNMAGA
                     WKLPVVFILENNVYSELTPIADMVSDPDLSRRGEPFGIPGLRVDGNDPEAVADAAGAA
                     IRKARAGEGPTLIQADTYRIAGHYIGDAETYRDEAELEEARRAEPLVVARARLLDLGV
                     PPADVETTERRVEEELKTAVATALASPPSDPEKIREHLYA"
     gene            complement(1391..2692)
                     /locus_tag="VFB52_13510"
     CDS             complement(1391..2692)
                     /locus_tag="VFB52_13510"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZO07400.1"
                     /translation="MSPGRAAAVTPPQPGGQLEWQNDLYQMACTQFRGVARTLDLDPD
                     QYTRLIEPRRSLIVNLPIRLDSGELVNFTGYRVQHILTMGPTKGGLRFAPDLTLGQCG
                     ALAMWMTWKCALLGLPYGGAKGGVRCDPHALSVDEIERITRRYAAEVNPIVGPDEDIP
                     APDMGTGEREMAWFYDTYSQAARHAVPACVTGKPVALGGTPGRRQATGLGVVFTLEAA
                     LESEERPLEEQSFAIQGFGNVGAVVASELHARGARVVGIGDIGGAVSNPAGLDVPAVA
                     RWYFEHDTVAGFPGAETIAREELLELSCDVLVPAALEHQITEANAGRLRCRMVAEGAN
                     GPTTPVADEILAERGITVLPDILVSGGGVTVSYFEWVQAHQKYVWTESEIRERLRSQM
                     RQALTSVIGTAEQLDVDLRTGALAVAVRRVAETAKLRAVFP"
     gene            complement(2689..4251)
                     /locus_tag="VFB52_13515"
     CDS             complement(2689..4251)
                     /locus_tag="VFB52_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO07401.1"
                     /translation="MTDFRNETLLELRRPHERERLLAALASLDAGPPLAVPVILGGER
                     RLDPTLASVDPGDPDRVVAAATVASAAEVDDAVAAATAAAGPWAARPAAERAAILSRA
                     AGELRGRRYELAALAVRECAKPWGEADADVCEAIDFLEYYGRQAVALEAGPELVQLPG
                     ERNTLHYRPRGVVGVIAPWNFPLAIAIGMTAAGLATGNAVCLKPAEQSPGCALALYEA
                     LERAGLPAGAVALLPGEGEVGAALAGHPGVQTIAFTGSGEVGMEIMRRAAEPVPGQLH
                     LKRVVAEMGGKNCIIVDSDADLDDVVPAVLASAFGFAGQKCSAAARVLVHERVAEQLS
                     ERLCGALETLMVGQAETFGTDVPPLIEASSQERVERVVAAAAKEGASAYRTLRVPESN
                     GYFAAPTVILGAGPEAPVAREEIFGPVVTVEPVAGVEAACDLVDRQPQALTGGLFSRN
                     PRTIAAVARRTPVGNPYVNRGTTGAMVGRQPFGGNRLSGNGAKAGGPDYLLHFVEPVV
                     VTENTVRHGLAP"
     gene            complement(4248..>4706)
                     /locus_tag="VFB52_13520"
     CDS             complement(4248..>4706)
                     /locus_tag="VFB52_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZO07402.1"
                     /translation="VPLTVRLVKGAYWDHETIESAQNGWVPPVWTEKVESDRCFERLS
                     RRLLDAFPLVRTAIASHNLRSVAHAVAYARHLGLGPADLEVQVLRGLGDDLQDAVTGA
                     GLRCRTYCPVGDIVAGMAYLVRRLLENTSNTSFLASRANGSDLDQLLARP"
BASE COUNT          669 a         1679 c         1697 g          661 t
ORIGIN      
        1 tggaggcggc cctcggacac gtagcggacg ttcggcgcct ggttgacgat ctggtcgaag
       61 gcgacgagcg tgaactccgc ccacatcacc tcgacgatcg gccgccgacc gaccatcgcg
      121 gccccgaggg cagcgccgag gaacccggtc tcgctgatcg gggtgtcgaa aacgcgctcc
      181 ccgtgcttct cgcgcaggcc tcgcgtgcag ccgaacaccc cgccgggaat tgcgatgtcc
      241 tcgccgaaga gcaaggcctc ggggaaggac tccagggcgc ggtccagcgc ggcgttgacc
      301 gccttcgcgt aggtcagtcg ctcgagcgct gctgcggtct caggcataga ggtgctcccg
      361 gatcttctcg gggtccgacg gcgggctggc aagggccgtc gcgaccgcgg ttttcagttc
      421 ctcttcgacc cggcgctccg tcgtctcgac gtccgccggc ggtacgccca ggtccagcag
      481 acgggcgcgg gcgacgacca gcggctcggc gcgccgagcc tcctccagct ccgcctcgtc
      541 ccgataggtt tcggcatcgc cgatgtagtg gccggcgatt cggtacgtgt cggcctggat
      601 cagcgtcggg ccctccccgg cccgcgcttt gcggatcgcc gctccggctg cgtcggcgac
      661 ggcttccggg tcgttgccgt cgacccgcag acccggaatc ccgaacggct cgccgcgccg
      721 cgagaggtca gggtcggaga ccatgtccgc gatcggcgtc agctcggagt agacgttgtt
      781 ttcgaggatg aagaccaccg gcagcttcca ggcaccggcc atgttgaagg cctccgagac
      841 ggcgccctgg ttcgccgcgc catccccgaa gacggtgatc gcgacccgat cgctgtcgtc
      901 gtagcgggcg gcgagcgcgg ccccgacagc catcgggacc tgagcgccga tgatcgcgtt
      961 ctcgccgtag aagccccact cgggcgccga gaagaaggcg gatccgccgc ggccgcctga
     1021 aacgccgagg gcccgggccg cgagctcggc caggacgttc tcaggcggcg ctccgcaggc
     1081 gagggcccaa ccgtgacccc ggtagttgcc gaagacgggg tcctgggggg agcgttcggc
     1141 gacaacgcca acgggcaccg cctcctgtcc gcaggagaga tggaccgagc cgaccacctg
     1201 accatcctca cgcagatcct cgatcgcttc ctcggtgcct ctgatgcgaa gcatctgctc
     1261 gtagaagccc agcagcctgg tgccgtccat caattccttc ctccgtcaga cgcttgtctc
     1321 agcgcattca ccctagcgct ttatgatcac tatacgaaca acgtatcaga gttcgacgaa
     1381 ctatgccctg ctaaggaaag acggcgcgca gcttcgcggt ctcggcgacg cgtcgcaccg
     1441 ccaccgccag ggcgccggtc ctcaggtcga catcgagctg ctcggcggtg ccgatcaccg
     1501 aagtcagcgc ctgccgcatc tggctacgca gccgctcgcg gatctcggac tcggtccaga
     1561 cgtacttctg gtgcgcctgc acccactcga aatagctgac ggtgacgccg ccgccgctga
     1621 ccaggatgtc gggcaggacg gtgatgccgc gttcggcgag gatctcgtcc gccaccgggg
     1681 tggtcggccc gttggcgccc tcggcgacca tgcggcagcg caggcggccg gcattggcct
     1741 ccgtgatctg atgctcgagc gcggccggga caagcacgtc acaggacagc tccagcagct
     1801 cctcgcgcgc gatcgtctcg gcgccgggga agccggcaac cgtgtcgtgc tcgaagtacc
     1861 agcgggcgac cgcggggacg tcgagcccgg ccgggttgga gaccgcgccg ccgatgtcgc
     1921 cgatcccgac gacccgggcg ccgcgcgcgt gcagctcgga ggcgaccaca gcgccgacgt
     1981 tgccgaagcc ctggatcgcg aagctctgct cctccagggg acgctcctcg ctttccagcg
     2041 ccgcctcgag cgtgaagacg acgccgagtc cggtcgcctg ccgtcgcccc ggggttcctc
     2101 cgagagcaac cggcttcccg gtcacgcagg ccggcaccgc gtggcgggcg gcctgcgagt
     2161 aggtgtcgta gaaccaggcc atctcgcgct cgcccgttcc catgtccggt gccgggatgt
     2221 cctcgtcggg gccgacgatc gggttcacct cggcggcgta gcgacgggtg atgcgctcga
     2281 tctcgtcgac cgagagcgcg tgcgggtcgc agcgcacgcc tcccttggcg ccaccgtagg
     2341 ggaggccgag cagcgcgcac ttccaggtca tccacattgc cagcgccccg cactggccga
     2401 gggtcaggtc cggggcgaag cgcaggccgc ccttggtcgg gcccatcgtg aggatgtgtt
     2461 ggacccggta gccggtgaag ttgaccagct cgccgctgtc gagccggatc ggcaggttga
     2521 cgatcagcga acggcgcggc tcgatcaggc gcgtgtactg atcggggtcg aggtcaaggg
     2581 tccgggcgac accgcggaac tgggtgcacg ccatctggta gaggtcgttc tgccactcga
     2641 gctggccgcc tggctgcggc ggggtcaccg ccgcagcccg ccccgggctc acggcgccaa
     2701 gccgtggcgg accgtgttct cggtcaccac gaccggctcc acgaagtgca gcagatagtc
     2761 ggggccgccg gccttggcgc cgttgcccga aagccggttg ccgccaaacg gctgacggcc
     2821 gaccatcgcc ccggtggtgc cgcggttgac ataggggttg ccgaccgggg tgcgccgggc
     2881 gaccgcggcg atcgtccgcg ggttgcgcga gaagaggccg ccggtcagcg cctgcggctg
     2941 gcggtcgacg aggtcgcagg cggcctcgac ccccgccacc ggctcgaccg tgaccaccgg
     3001 cccgaagatc tcctcgcgag cgacgggcgc ctcgggcccg gcgccgagaa tcacggtcgg
     3061 ggcggcgaag tatccgttgc tctcgggcac ccgaagggtg cggtaggcgc tcgcgccttc
     3121 cttcgccgcc gccgcgacca cgcgctcgac ccgttcctgg gagctggcct cgatcagcgg
     3181 cgggacatcg gtgccgaagg tctccgcctg gccgaccatc agcgtctcca gggcgccgca
     3241 gaggcgctcg gagagctgct cggcgacccg ctcgtggacc aggacccggg ccgcggccga
     3301 gcacttctgt ccggcgaagc cgaaagccga ggcgagcacc gccgggacga cgtcgtcgag
     3361 atccgcatcg gaatcgacga tgatgcagtt cttgccgccc atctcggcga ccactcgctt
     3421 caggtggagc tgcccgggga ccggctcggc ggcccggcgc atgatctcca tcccgacctc
     3481 gccggagccg gtgaaggcga tcgtctgcac acccggatgg ccggccaggg ccgcgccgac
     3541 ctcgccctcg cccggcagca gggcaacggc gccagccggc aggccggcgc gctcgagcgc
     3601 ttcgtagagg gcgagcgcgc agcccggcga ctgctcggcc ggcttcaggc agacggcgtt
     3661 gccggtggcc agccccgccg cggtcatccc gatcgcgatc gccagcggga agttccaggg
     3721 ggcgatgacg ccgaccaccc cgcgcggccg gtagtggagc gtgttgcgct cgccgggcag
     3781 ctggactagc tccgggccag cttccagcgc gacggcctgg cggccgtagt actcgaggaa
     3841 gtcgatcgcc tcgcagacgt cggcgtcggc ctcgccccac ggcttcgcgc actcgcgcac
     3901 ggccagcgcg gcgagctcgt agcgacggcc gcgcagctct ccggccgcac gggagaggat
     3961 cgccgcccgc tcggccgccg gccgcgccgc ccaggggccg gcggcagctg tggccgcggc
     4021 gacggcatcg tccacctcgg cggcgctggc cacggtcgcg gcggcgacca cgcggtccgg
     4081 gtcaccgggg tcgaccgagg ccagggtcgg atcgagccgg cgctcgccgc cgaggatcac
     4141 cgggacggcc agcggcggcc cggcgtcgag gctcgccagc gccgcgagca gccgctcgcg
     4201 ctcgtgcggg cggcgcagct cgagcagggt ttcgttgcgg aagtcggtca tgggcgggca
     4261 agcagctgat cgaggtcaga gccgttggcg cggctggcga ggaaggaggt gttggaggtg
     4321 ttctcgagca ggcgccggac caggtaggcc atcccggcga cgatgtcgcc caccgggcag
     4381 taggtgcggc agcgcagtcc ggcgccggtg accgcgtcct ggaggtcgtc gccgaggccg
     4441 cgcaggacct ggacctcgag gtcggcggga ccgagcccga gatggcgcgc gtaggcgacc
     4501 gcgtgggcga ccgagcgcag gttgtgcgag gcgatcgcgg tgcggaccag cgggaaggcg
     4561 tcgagcaggc gccggctcag ccgttcgaag cagcggtcgc tctccacctt ctcggtccag
     4621 accggcggca cccagccgtt ctgggcggac tcgatcgtct cgtggtccca gtacgcgccc
     4681 ttgaccagcc gcaccgtcag cggcac
//
LOCUS       DASRIG010000506         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2560564, whole genome shotgun sequence.
ACCESSION   DASRIG010000506 DASRIG010000000
VERSION     DASRIG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560564"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFB52_13525"
     CDS             <1..311
                     /locus_tag="VFB52_13525"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07403.1"
                     /translation="SYEQKHFDAAAKRGRLRLIASPDGRDGSVTIHQDALVYAGLFDG
                     RENGSYDLKNERGYVHVARGRITVNGEILQAGDALKTDGGLIELEQGENAEVLLFDLP
                     "
     gene            complement(313..1116)
                     /locus_tag="VFB52_13530"
     CDS             complement(313..1116)
                     /locus_tag="VFB52_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO07404.1"
                     /translation="MSQPLVYSLTAAGQEALSTEIGEADFPLEVNFPTDYKRLLAMIE
                     VGGHIEVIRGRLRRFPDRLIDEWLKELEDLKMVESHEAGDLDEITFTGRPLPLLPPMT
                     DDDRKRLAKTTVVGGATLLRSGSFLSEERIANLAPLAKAPAQTVILLVEDDPDQAALA
                     ELRLTMAGYQVHSVDRAKALSGYLRQQARPDLLLLDVMLPDGNGFDILAQLRGRPEFS
                     TLPIVLLTAKAELTDIRKGLALGADGYVTKPYSKNQLAEVIGRVLKHPA"
     gene            complement(1116..1895)
                     /locus_tag="VFB52_13535"
     CDS             complement(1116..1895)
                     /locus_tag="VFB52_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZO07405.1"
                     /translation="MAFVYHLTAAGKSALSTSDPKANFPLEYRRLLSLIEVEGHIDVI
                     RGRLRRFPDRFIEEWLKELEDLKMVESRPAEELDDFTFTGQRVPALPQLADEDSKRLA
                     KTAVVAGATLLRSGSFLADERIANLPPLAKAPADTVILLVEDDPDQMALAQLRLTMAG
                     YQVRSVDRAKALSRTLREEARPDLLLLDVMLPDGNGFEILALLRGHPDFATLPIVLLT
                     VKAELADIRNGLALGADGYITKPYSKNQLAEVIGRVLRHRT"
     gene            complement(1978..>2429)
                     /locus_tag="VFB52_13540"
     CDS             complement(1978..>2429)
                     /locus_tag="VFB52_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07406.1"
                     /translation="ITLPDGITRPYSVWLSGEYPRALDGLCKMLSLDMRVLDPAWVGM
                     KLRKLLEFPEPLGDFMAFVPGSRKQQTFPSTVSYLARLILHRYAMLGLLNEEGIARNQ
                     MGILEAPAAKAAPLMAGAKCPECANPTLIRKDGCDYCTACGYVGVCG"
BASE COUNT          393 a          765 c          882 g          389 t
ORIGIN      
        1 cttcctacga gcagaagcac ttcgacgccg ccgccaagcg cggacggctg cggctcatag
       61 cctcgcccga cggccgcgat ggctcggtca cgatccacca ggacgcactg gtctacgccg
      121 gcctgttcga cggccgcgaa aacgggagct acgacctgaa gaacgagagg ggctacgttc
      181 acgtcgcgcg cgggcgcatc accgtgaacg gcgagattct ccaggcgggc gacgcgctca
      241 agaccgacgg tgggttgatc gagctggagc agggcgagaa cgcggaagtc ctgctgttcg
      301 acctgcccta ggctaggccg ggtgcttgag cacccggccg atgacctcgg cgagctggtt
      361 cttgctgtag ggcttggtga cgtagccgtc ggcgccgagc gcgagaccct tgcggatgtc
      421 ggtcagctcg gctttcgccg tgaggagcac gatcggcagc gtggagaact cggggcggcc
      481 gcgcagctgc gccaggatgt cgaagccgtt gccgtccgga agcatcacgt cgagaagcag
      541 caggtcgggg cgcgcctgct ggcgcaagta gcccgagagc gctttcgcgc gatccacgct
      601 gtgcacctgg tagccggcca tggtcagccg cagctccgcg agcgccgcct ggtcggggtc
      661 gtcctccacc agcaggatta ccgtctgggc cggcgccttg gcgagcggag ccaggttggc
      721 gatgcgctct tccgagagga aggaacccga gcgcagcagc gtcgcgccgc cgaccaccgt
      781 ggtcttcgcc aggcgcttgc ggtcgtcgtc ggtcatcggc ggcagcagcg gcagggggcg
      841 gccggtgaag gtgatctcgt cgaggtcgcc cgcctcgtgc gactcgacca tcttcaggtc
      901 ctcgagctcc ttcagccact cgtcgatcag ccggtcgggg aagcggcgca gccgcccgcg
      961 gatcacctcg atgtggccgc cgacctcgat catggcgagg agccgcttgt agtcggtggg
     1021 aaaattgacc tcgaggggaa aatcggcctc gccgatctca gtcgacaggg cttcctgccc
     1081 tgcggcggtc agggaataga ccaggggctg ggacatcagg tgcgatgcct gaggacgcgg
     1141 ccgatgacct cggcgagctg gttcttgctg tagggcttgg tgatgtagcc gtcggcgccg
     1201 agcgcgaggc cgttgcggat gtcggcgagc tcggccttca ccgtcagcag cacgatcggc
     1261 agcgtggcga agtcgggatg gccgcgcagc agggcgagga tctcgaagcc gttgccgtcg
     1321 gggagcatga cgtcgagcag cagcaggtcg gggcgcgcct cctcgcgcag ggtgcgcgac
     1381 agcgccttcg cgcggtcgac gctgcgcacc tggtagcccg ccatcgtcag ccgcagctgg
     1441 gcgagcgcca tctggtccgg gtcgtcctcc accagcagga tcacggtatc ggccggcgcc
     1501 ttggcgagcg gcggcaggtt ggcgatgcgc tcgtccgcca ggaacgagcc cgagcgcagc
     1561 agcgtggcgc cggcgacgac cgcggtcttg gcgaggcgct tgctgtcctc gtcggcgagc
     1621 tgcggcagcg cgggcacgcg ctggccggtg aaggtgaagt cgtcgagctc ctccgccggc
     1681 cgcgactcga ccatcttcag gtcctcgagc tccttcagcc attcctcgat gaagcggtcg
     1741 ggaaagcggc gcagccgccc gcggatcacg tcgatatggc cctcgacctc gatcaggctg
     1801 aggaggcggc ggtactcgag gggaaaattc gccttgggat cactcgtcga cagagcgctt
     1861 ttccccgcgg cggtcaggtg ataaacgaag gccatgtgat gcgaattgtg aacgatgcgc
     1921 tacctcggcg aaaggtgacc tggttcattc gccgaggtta acaattgtag gaaccggcta
     1981 cccgcaaacc cccacatagc cgcaagcggt gcagtagtcg cagccgtcct tgcggatgag
     2041 ggtggggttg gcgcactcgg ggcacttggc gccggccatc aagggagcgg ccttggccgc
     2101 gggcgcttcg aggatgccca tctggttccg ggcgatgcct tcctcgttca ggagcccgag
     2161 catcgcgtag cggtgcagga tcaggcgcgc gaggtaggac acggtggagg ggaacgtctg
     2221 ctgtttgcgg ctgccgggga cgaaggccat gaagtcgccg agcggctcgg ggaactccag
     2281 cagcttcctc agcttcatcc cgacccacgc cgggtcgagc acgcgcatgt cgagcgacag
     2341 catcttgcac aggccgtcga gcgcgcgcgg atattcgccg gagagccaga cggagtaggg
     2401 ccgggtgatc ccgtccggca aggtgattt
//
LOCUS       DASRIG010000507         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1539566, whole genome shotgun sequence.
ACCESSION   DASRIG010000507 DASRIG010000000
VERSION     DASRIG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539566"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="pabB"
                     /locus_tag="VFB52_13545"
     CDS             complement(<1..1359)
                     /gene="pabB"
                     /locus_tag="VFB52_13545"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZO07407.1"
                     /translation="MELRQPRLLVRRIDGDRDAELAFARLYGERSDAFWLDSSRVGEG
                     ARFSFLGAPGPLGEVITRRVGEGESIFDHLSEQLQSLRLDSPELPFGFNCGFVGWFGY
                     ELKAETGGRAAHAASTPDAAFLFVDRLVAFDHEDGRTYLLCLAEEAGEGPGGIADGER
                     WLAETAALLEALPPSHGEPERIESAGEPVEFHLARSREHYLEDIAACKRALYEGESYE
                     ICLTNQVRAAVAPEPLALYRALRRVNPAPYAALLRIGSTAVLSSSPERFLRIGADGAV
                     EAKPIKGTSPRAETPEADEALAEALRTSEKNRAENLMITDLLRNDLGTVCEVGSVHVP
                     HLMAIETFETVHQLVSTVRGRLRDGLDAVDCIRACFPGGSMTGAPKKRTMEIIDELET
                     EARGVYSGAIGYIGLGGAADLNIVIRTVVMSRDETSIGVGGAIVMQSDADEEYQETLL
                     KAQ"
     gene            complement(1350..1943)
                     /locus_tag="VFB52_13550"
     CDS             complement(1350..1943)
                     /locus_tag="VFB52_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZO07408.1"
                     /translation="MRTLLIDNHDSFTYNLFHLLAEVNGCEPVVVRNDEASWEELAAL
                     GADNVVLSPGPGRPEREADFGVCAEAIRRAEVPLLGVCLGHQGLGWASGAAVVHAPAP
                     VHGGIVGVRHAGASLFAGIPAEFNAVRYHSLCLVEPLPEELEPIAWGEDGVLMAIAHR
                     RLPRWGVQFHPESVASEHGRRLLANFRDLTAGCARWN"
     gene            1965..2708
                     /locus_tag="VFB52_13555"
     CDS             1965..2708
                     /locus_tag="VFB52_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZO07409.1"
                     /translation="MHPPTPPDPSRGLFETLLIAGERPVEQVAHFGRLANSARELFGA
                     ELPAELPGDAVRASAGIELGRLRIDVEPDGAGGLRHKLQAVAIDRDAFFPDWEHGEAL
                     RSVGAEGWSGAHKWSDRRWLEDAEAELGDEVPLLVDADGTVLEAGRANLFIVADGGLA
                     TPPADGRILPGTARAATLEIAADLGIPATERPLTLADLRGADEVFLTSSVRGLRPARY
                     LDGEELSHGDLVARLAAELRQRWLGSRAL"
     gene            complement(2705..>3286)
                     /locus_tag="VFB52_13560"
     CDS             complement(2705..>3286)
                     /locus_tag="VFB52_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07410.1"
                     /translation="NRVFGEYSEDSEGLLTLEEAFDGALVFEVGSSGQAGVTGGGVNI
                     DDVPLSSYAGEFHALAIAPRFMQMNDIALRRGEVEEGAQRTLELSENYGAPGSWTAIS
                     PSMVTISAAETIEVPNGVSFVKISGTTAVKTITPTTPGHVLTAQLITAGGNIYTMVGN
                     LKLSSNHGAGNLRGTLTMICDGTYWYELSHSNN"
BASE COUNT          462 a         1213 c         1128 g          483 t
ORIGIN      
        1 ctgggccttg agcagcgtct cctggtactc ctcgtcggca tcggactgca tcacgatcgc
       61 cccgccgacg ccgatcgacg tctcgtcgcg cgacatcacg acggtccgga tcacgatgtt
      121 gaggtcggcg gctccgccga ggccgatgta gccgatcgct ccggagtaca cgcctcgcgc
      181 ctccgtctcg agctcgtcga tgatctccat cgtccgcttc ttcggcgcgc cggtcatcga
      241 cccgccgggg aagcacgcgc ggatgcagtc cacggcgtcc agcccgtccc tgaggcgccc
      301 gcggacggtc gagacgagct ggtgcactgt ctcgaacgtc tcgatcgcca tgaggtgcgg
      361 aacgtgcacg ctgccgacct cgcacaccgt gcccaggtcg tttcgcagca ggtcggtgat
      421 catcaggttc tcggcccggt tcttctcgct ggtgcgcaga gcctcggcca gggcctcgtc
      481 cgcctcgggc gtctccgcac gggggctcgt ccccttgatc ggcttcgcct cgaccgcgcc
      541 gtcggcgccg atccgcagga agcgctccgg cgaggagctc agcaccgcgg ttgagccgat
      601 ccgcagcagc gccgcgtagg gcgccgggtt gacccggcgc agggcgcggt agagcgccag
      661 cggctcgggc gcgaccgcgg cccgcacctg gtttgtcagg cagatctcgt aggactcgcc
      721 ctcgtacagc gcgcgcttgc aggcggcgat gtcctcgagg tagtgctcgc gtgagcgcgc
      781 gaggtgaaac tcgaccggct ccccggccga ctcgatccgc tccggctcgc catggctcgg
      841 cggcagcgcc tccagcagcg ccgcggtctc ggccagccag cgctcgccgt cggcgatgcc
      901 gccgggcccc tcccccgcct cctcggcgag gcagagcagg taggtgcgcc cgtcctcgtg
      961 gtcgaaggcg acgagccggt cgacgaagag gaaggcggcg tcaggggtgc tggcggcgtg
     1021 ggcggcgcga ccgccggtct ccgccttcag ctcgtagccg aaccagccga cgaagccgca
     1081 gttgaagccg aacggcagct ccggcgagtc gagtcgcagg ctctggagct gctctgagag
     1141 gtggtcgaag atcgactcgc cctcccccac ccgccgcgtg atcacctcgc cgagcgggcc
     1201 gggggcgccg aggaacgaga agcgcgcgcc ctcgccgacc cggctgctgt cgagccagaa
     1261 ggcgtcgctg cgctcgccat agagccgcgc aaaggcgagc tcggcgtcgc ggtcgccgtc
     1321 gatccggcgg accagcaggc gcggctgcct cagttccaac gtgcgcaccc cgctgtcagg
     1381 tcgcggaagt tggcgagcag ccggcgcccg tgctcggaag cgaccgactc gggatggaac
     1441 tggacccccc agcgcggcag ccggcggtgc gcgatcgcca tcagcacccc gtcctcgccc
     1501 caggcgatcg gctccagctc ctcgggcagc ggctcgacga ggcagagcga gtggtagcgg
     1561 accgcgttga actcggccgg gatcccggca aacagcgagg cgcccgcgtg gcggaccccg
     1621 acgatcccgc cgtggaccgg tgcgggcgcg tgcacgaccg cggccccgga tgcccagccc
     1681 agcccctggt ggccgaggca gacgcccagc agtggcacct cggcgcgccg gatcgcctcc
     1741 gcgcagacac cgaagtcggc ctcgcgctcg ggccggccgg ggccgggcga gagcaccacg
     1801 ttgtcggcgc cgagcgccgc cagctcctcc cagctcgcct cgtcgttgcg caccaccacc
     1861 ggttcgcacc cgttcacctc ggccagcagg tggaagaggt tgtaggtgaa tgaatcgtgg
     1921 ttgtcgatca gcaacgtgcg cacgtgaaca cggatacttg cgcggtgcac ccgccgacgc
     1981 cacccgaccc cagccgggga ctgttcgaga cgctgctgat cgccggcgaa cggccggtcg
     2041 agcaggtcgc ccacttcggc cgcctcgcca acagcgcccg cgagctgttc ggcgccgagc
     2101 tgcccgccga gctgcccggc gacgcggtcc gcgccagcgc cgggatcgag ctcggccgcc
     2161 tgcggatcga cgtcgaaccc gatggcgccg gcggcctgcg ccacaagctc caggcggtcg
     2221 cgatcgaccg cgacgccttc ttccccgact gggagcacgg cgaggcgctg cgcagcgtcg
     2281 gcgccgaggg ctggtcgggc gcgcacaagt ggtcggaccg ccgctggctc gaggatgccg
     2341 aggccgagct cggcgacgag gtgccgctgc tggtcgacgc cgacggcacg gtgctcgagg
     2401 caggccgcgc caacctcttc atcgtcgcag acggcggcct cgccaccccg cccgccgacg
     2461 gccgcatcct ccctggcaca gcccgggccg cgaccctcga gatcgcggcc gacctcggca
     2521 ttcccgccac cgagcgcccc ctcaccctcg ccgacctgcg gggcgccgac gaggtcttcc
     2581 tcacctcatc ggtgcgcggc ctgcgccccg cccgttacct cgacggcgag gaactcagcc
     2641 acggcgacct ggtcgcgcgc ctcgccgccg agctgcgcca gcgctggttg ggcagccggg
     2701 cgctctagtt gttgctgtgg gacagctcgt accagtaggt gccgtcgcag atcatcgtca
     2761 gggttcccct gagattgcca gcaccgtgat tgctggacag tttgaggttc cccaccatgg
     2821 tgtagatgtt cccgcccgca gtgatgagct gagcggtgag cacgtgccct ggcgtggtcg
     2881 gcgtgatcgt tttgaccgcc gtggtcccgc tgatcttcac aaacgaaact ccgttgggca
     2941 cttcgatcgt ttccgcggct gagatcgtga ccatgctcgg cgagatcgcc gtccaggacc
     3001 cgggtgcacc gtagttttcg gagagctcca gcgtccgctg agccccttct tcgacctcgc
     3061 cacgccgcag cgcgatgtcg ttcatctgca tgaaccgcgg cgcgatcgcg agtgcgtgga
     3121 actcgcccgc gtaagaggag agcggaacgt cgtcgatgtt cacaccgcca ccggtgacgc
     3181 ccgcctggcc ggagctgccg acctcgaaga cgagggcgcc gtcgaaggct tcttccaggg
     3241 tcagcagccc ttccgagtcc tccgagtatt cgccgaagac ccggtt
//
LOCUS       DASRIG010000508         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_999441, whole genome shotgun sequence.
ACCESSION   DASRIG010000508 DASRIG010000000
VERSION     DASRIG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999441"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFB52_13565"
     CDS             <1..412
                     /locus_tag="VFB52_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO07411.1"
                     /translation="SAVGDRDRSEAGGLQTTAQQLGSSLGTALLGAIVITGLITAFTT
                     NVNDNPRIAADVKQQVQVQLSAGASFVAADQVEAAAQDVGVDEATTAELVSDYEDAQL
                     KALKTAFLVAALLVLASFFATRNLPSRRFDELQT"
     gene            complement(409..1785)
                     /locus_tag="VFB52_13570"
     CDS             complement(409..1785)
                     /locus_tag="VFB52_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZO07412.1"
                     /translation="MNGPERVARGKAARSEARRSAHAEWQPAENRPDPIAVLEEQAAS
                     RVQNLVPIRYGRMAVSPFTFYRGAAAVMSADLATTPDSGLPVQACGDAHLSNFGAFAA
                     PDRRLVFDLNDFDESLPGPWEWDVKRLAASFVVATRESGLKRKQREALVLKVVGSYRE
                     AMREFAAMRNLEVWYARLDAEEALAEVAEQDPDEVEKVRKGVAKARAKDSLRALEKLT
                     ETVDGEIRFRSEPPILVPGEELLPAGDGRSVEELIADVLVAYRDTLAADRQHLLDRYR
                     FRQLAHKVVGVGSVGTRAWVVLLTGDDDSDPLFLQAKEAQASVMEPYVGASRYRNHGR
                     RVVEGQRLMQAASDIFLGWCPAVGLDGQQRHFYVRQLWDWKRSAEIESLSPRGFEVYA
                     RICGWTLARAHARSGDRVAIAAYLGGGDSFDRAIATFSEVYADQNERDHEALLAAIDE
                     GRIEAVDA"
     gene            complement(1789..>2499)
                     /locus_tag="VFB52_13575"
     CDS             complement(1789..>2499)
                     /locus_tag="VFB52_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07413.1"
                     /translation="EGGHRGISAFVVDADLPGVSVDKKEDKMGQRASNTATISFDEVE
                     IPAENLLGEENKGFKLAMMTLDRTRPGVSAMAVGIGRAALEYATEYSKERVQFGVPIA
                     MHQAIQFMIADMATKVEAARLLVWSSAVMLDQGIRNSKVSSMAKRFAADSAMEITVDA
                     VQVYGGYGFIKEYPVEKLMRDAKIMQLYEGTSQIQRLVIAKEVLIPRPAGERTPEAEA
                     KAADAARDSQSEQAAATV"
BASE COUNT          353 a          874 c          871 g          401 t
ORIGIN      
        1 gtcggcggtc ggcgaccgtg accgcagcga ggccggcggg ctacagacca ccgcgcagca
       61 gctcggctcc tcgctgggca cggcgctgct cggggcgatc gtgatcaccg gcctgatcac
      121 cgccttcacc accaacgtca acgacaaccc gcggatcgcc gccgacgtca agcagcaggt
      181 gcaggtgcag ctcagcgccg gggccagctt cgtcgctgcc gatcaggtgg aagcggcggc
      241 ccaggacgtc ggcgtcgacg aggcgaccac cgcagagctc gtctccgact acgaagacgc
      301 ccagctgaag gcgctgaaga ccgccttcct ggtcgccgcc ctgctcgtgc tcgcctcctt
      361 cttcgccacg cgcaacctgc cctcgcgccg cttcgacgag ctgcagacct aggcgtcgac
      421 cgcctcgatc cggccctcgt cgatcgccgc cagcagcgcc tcgtggtcgc gctcgttctg
      481 gtcggcgtaa acctccgaga aggtcgcgat cgcgcggtcg aaggagtcgc cgccgccgag
      541 gtaggcggcg atcgcgaccc ggtcgccgga gcgggcgtgg gcgcgggcca gcgtccagcc
      601 gcagatccgg gcgtagacct cgaagccgcg cgggctgagg ctctcgatct cggccgagcg
      661 cttccagtcc cagagctggc gcacgtagaa gtggcgttgc tggccgtcga gcccgaccgc
      721 cgggcaccag ccgaggaaga tgtcgctggc cgcctgcatc aaccgctgcc cctcgaccac
      781 gcggcggccg tggttgcggt agcggctggc gccgacgtag ggctccatga ccgaggcctg
      841 cgcttccttc gcctgcagga acaacgggtc ggagtcgtca tccccggtca gcagcacgac
      901 ccaggcgcgg gtgccaacgc tgccgacccc gactactttg tgggcgagct ggcggaagcg
      961 gtagcggtcg agcaggtgct ggcggtcagc cgccagggtg tcgcggtagg cgacgaggac
     1021 gtcggcgatc agctcctcga cgctgcgccc gtcgccggcc ggcagcaact cctcgccggg
     1081 gacaaggatc ggcggttcgc tgcggaagcg gatttccccg tcgaccgtct cggtcagctt
     1141 ctcgagcgcc cgcaggctgt ccttcgcgcg cgccttggca actcccttgc gcactttctc
     1201 gacttcgtcg gggtcctgct cggcaacctc ggccagggct tcttcggcgt cgaggcgcgc
     1261 gtaccagacc tcgaggttgc gcatcgccgc gaactcgcgc atcgcctcgc ggtaggagcc
     1321 gacgaccttc agcaccagcg cctcgcgctg tttgcgtttg aggccgcttt cgcgggtggc
     1381 gacgacgaag ctggcggcga gccgcttgac gtcccactcc cacggccccg gcagtgactc
     1441 gtcgaagtcg ttgaggtcga agacgaggcg gcggtcgggc gccgcgaagg caccgaagtt
     1501 ggagaggtgg gcgtcgccgc aggcctgcac gggcaggccg gagtcgggcg tggtcgccag
     1561 gtccgcggac atcaccgcgg cggcgccgcg gtagaaggtg aacggcgaga ccgccatccg
     1621 cccgtaacgg atcgggacca ggttctggac ccgcgacgcg gcctgctcct cgagcaccgc
     1681 gatcgggtcc ggccggttct cggccggctg ccactcggcg tgggccgagc gacgggcctc
     1741 cgagcgcgcc gccttacctc gggcgacgcg ctcgggaccg ttcatgcctt aaaccgtagc
     1801 cgctgcctgc tcagactgcg agtcacgggc ggcgtcggcg gccttcgcct cggcctccgg
     1861 ggtgcgctcg ccggcggggc gcggaatcag gacttctttg gcgatcacga ggcgctggat
     1921 ctggctggtg ccctcgtaga gctgcatgat cttcgcgtcg cgcatcagtt tctcgaccgg
     1981 gtactccttg atgaagccgt acccgccgta gacctggacg gcgtcgacgg tgatctccat
     2041 cgccgagtcg gcggcgaagc gcttggccat cgaggagacc ttgctgttgc ggatgccctg
     2101 gtcgagcatg accgcggagc tccagaccaa caggcgcgca gcctcgacct ttgtggccat
     2161 gtcggcgatc atgaactgga tcgcctggtg catggcgatc ggcacgccga actggaccct
     2221 ttccttggag tactccgtgg cgtactcgag cgcggcgcgg ccgatgccga ccgccatcgc
     2281 cgagacaccc gggcgggtgc ggtcgagggt catcatcgcc agtttgaagc ccttgttctc
     2341 ctcgccgagg agattctcag cggggatctc gacttcgtcg aaggagatgg tcgcggtgtt
     2401 cgacgcccgc tggcccatct tgtcctcctt cttgtcgact gagaccccgg ggaggtcggc
     2461 gtcgacgacg aaggcgctga tcccgcggtg cccgccctc
//
LOCUS       DASRIG010000509         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3103019, whole genome shotgun sequence.
ACCESSION   DASRIG010000509 DASRIG010000000
VERSION     DASRIG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103019"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFB52_13580"
     CDS             complement(<1..1290)
                     /locus_tag="VFB52_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO07414.1"
                     /translation="MKRIAAILLAVGAAIAVLAVGTAAGGDDDVYEVRAIFDNAGFLV
                     DGEEVRVAGANVGVVTDVTVTGNDEPALEDGGADPGKAAVVMRIDDPGFQDFREDASC
                     LIRPQSLIGEKFVECEPTQPHAASSQPPPPLEPIPDGEPGEGQYLLPLERNGKAVDLD
                     LVNNIMEEPYPDRFRLILNDLGAGLAARGEELAEIIERANPALRETNEVLAILARQNR
                     VLAQLSADSDVVLGALARERAQISSFINQSDVVAAATAERSADLEESFARFPSFLREL
                     RSTMDRLDAFSTAATPVFTDLGDTAPSLTRLSRDLEPFSAASTRALTSLGTAAAGSGP
                     NIAASDPVIRQVRGLAKTAQPAAANLRKLLASVRETDGFSNLTELVFNGAGAVNPFDQ
                     YGHFIRGLLPTNNCVDYDVIPEASCSANFVGATTTAAA"
     gene            complement(1287..2600)
                     /locus_tag="VFB52_13585"
     CDS             complement(1287..2600)
                     /locus_tag="VFB52_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZO07415.1"
                     /translation="MVAVVLVVVLLLGDDDGHEYELLFETGGQLVPGNQVLVGGQAVG
                     TIDEISLTEDAQAKVKITTDDPLHEGTTAVIRSTSLSGIANRYISLSPGPNNEPEIAD
                     GGVIAGDRTTSPVDLDQLFNTLDERTRAALQDVIKGQATIYTGNNAAARRTYKYFAPG
                     LQATERLLAELTRDQASLSRFLVEGSTALGAIAERRDDLAALTSNANEALGAIAERNE
                     EFDRTLVALPPALLQANTTFVNLRAALDDLDPLIQDLGAVAPDLPGFLRLTRETADPA
                     GPVFANLTQAIARPGKANDLTDSLRSAPAAQRQASRSVAPSITALDASQPNIELLRAY
                     SPDVLSLAGRLGQVTAYYDANGHYARVIPAGANLFSNAGGTLTPIAPAQQFDQYSALG
                     IGPFTRCPGAATQANAGWPSPTDHPFLGGRLLDGKCDPTDVPPGP"
     gene            complement(2877..3701)
                     /gene="rsmI"
                     /locus_tag="VFB52_13590"
     CDS             complement(2877..3701)
                     /gene="rsmI"
                     /locus_tag="VFB52_13590"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZO07416.1"
                     /translation="MPGRLTVCPTPIGNVDDLSPRARRALVEADIVACEDTRRAGRLF
                     ERLELERPRLVSNYEGNEAERARQLAQQIERGARVVLISDAGTPVISDPGFRLIRACL
                     ERDLEVEVLPGPSAVTTALVASALPADRWRFEGFLPRRAGELERVLGSPETVVAFESP
                     RRLPASLAALAALAPDRPAAVCRELTKLHEEVSRGTLGELARRYRGEAKGEIVIVIGP
                     ATSAGAGPDVAFAVDALRRLVQSGARPRAAASVVAALTGTRANDLYRALTGRDPRE"
     gene            complement(3766..>4458)
                     /locus_tag="VFB52_13595"
     CDS             complement(3766..>4458)
                     /locus_tag="VFB52_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZO07417.1"
                     /translation="IVDVPFRELVEQRLNLPTFLDNDANLAALAEHRFGAAKGATNAV
                     LLTIGTGIGGGLVLGGELYRGSTGAGAELGHVVIDQAGPPCQGNCPNRGCVEVLASGT
                     ALGREGRIAAETHPGSSLGRALAAGEEITGRLVTKHAIDGDGIAVEVVATIGRRLGVA
                     LSSLANIFDPDVIVIGGGVSAAGDLLLEPAREQLRSRALPPMNETPVLPAAMGPDSGF
                     IGAAEMALEETA"
BASE COUNT          613 a         1529 c         1657 g          659 t
ORIGIN      
        1 ggccgccgcg gtggtggtcg cgccgacgaa gttcgcgctg cagctagcct cggggatgac
       61 gtcgtaatcg acgcagttgt tggtcggcag caggccccgg atgaagtggc cgtattggtc
      121 gaacgggttg acggccccgg caccgttgaa tacgagctcg gtcaggttgc tgaacccgtc
      181 cgtctcgcgc acgctcgcga gcagcttgcg caggttggcg gcggccggct gggcggtctt
      241 cgcgagccct cggacctggc gaatcaccgg atccgaggcg gcgatgttcg ggccgctgcc
      301 cgcggcggcc gtcccgagac tggtcagggc gcgcgtgctt gcggcggaga atggctcgag
      361 gtctcgcgac aaccgagtca gcgagggagc cgtatcgccg aggtcggtga agaccggcgt
      421 cgcggcggtc gagaaggcgt cgagcctgtc catcgtcgag cgcagttcgc gcaggaagct
      481 cgggaagcgc gcgaacgact cctcgaggtc ggcgcttcgc tcggcggtcg cggcggcgac
      541 cacatcggac tggttgatga agctggagat ttgagctcgc tcccgggcca gggccccgag
      601 cacgacgtca gagtcggcgg agagctgcgc gagcacccgg ttctggcggg cgaggatggc
      661 gaggacctcg ttggtctcgc gcagcgccgg gttggcgcgc tcgatgatct cggccagctc
      721 ctcgccacgg gccgcgagcc cggcgccgag gtcgttgagg atgaggcgga accggtccgg
      781 gtagggctcc tccatgatgt tgttgaccag gtcgagatcg accgccttgc cgttgcgctc
      841 cagcggcagc aggtactggc cctcgcccgg ctcgccgtcg gggatcggct caagcggcgg
      901 cggcggctgg ctgctcgcgg cgtgcggctg ggtgggttcg cattcgacga acttctcgcc
      961 gatcagcgac tgggggcgga tcaggcagct cgcgtcctcg cggaagtcct ggaacccggg
     1021 atcgtcgatc cgcatcacga ccgcggcctt gccgggatcg gcgccgccgt cctccagcgc
     1081 gggctcgtcg ttgccggtca cggtcacgtc ggtgactacg ccgacgttgg ccccggcgac
     1141 ccggacctcc tcgccgtcga ccaaaaaccc ggcgttgtcg aagatcgcgc ggacctcgta
     1201 gacgtcgtca tccccgccgg cggccgtacc gacggccaga accgcgatcg cggctccgac
     1261 ggcgagcagt atggccgcga tccgcttcat ggtcccgggg gcacgtctgt ggggtcgcac
     1321 ttgccgtcga gcaacctgcc gccgaggaag ggatggtcgg tcggcgacgg ccagccggca
     1381 ttcgcctggg tcgcggcgcc ggggcagcgg gtgaacggtc cgatcccgag cgccgagtac
     1441 tggtcgaact gctgcgccgg tgcgatcggc gtcagcgtcc cgcccgcatt cgagaacagg
     1501 ttcgcgcccg caggaatgac gcgcgcgtag tggccgttgg cgtcgtaata ggcggtgacc
     1561 tggccgagcc gtccggccag gctgagcaca tccggcgagt aggcccggag cagctcgatg
     1621 ttcggctggg aagcgtcgag cgcggtgatc gacggagcca ccgatcgact cgcctggcgc
     1681 tgcgccgcgg gggcgctcct gagcgaatca gtgaggtcgt tggccttgcc gggccgggcg
     1741 atcgcctgcg tgaggtttgc gaagaccggg ccggccgggt cggcggtctc acgggtcagg
     1801 cgcaagaagc cgggcaggtc gggggcgacc gcgcccagat cctggatcag cggatcgagg
     1861 tcgtcgagcg cggcccgcag gttgacgaaa gtcgtgttcg cctgaagcaa cgcgggcggc
     1921 agcgcgacga gggtccgatc gaactcctcg ttgcgctcgg cgatcgcccc gagggcttca
     1981 ttcgcgttcg aggtcagcgc cgcgaggtcg tcgcggcgct cggcgatcgc cccaagggcg
     2041 gtcgagccct cgaccaggaa gcggctcagc gacgcctggt cacgggtgag ctcggcgagc
     2101 agccgctcgg tcgcctgcag gccgggagcg aagtacttgt aggtgcgccg cgcggcggcg
     2161 ttgttgccgg tgtagatcgt cgcctggccc ttgatcacgt cctggagcgc cgcgcgagtg
     2221 cgctcgtcga gggtgttgaa gagctggtcg aggtccaccg gcgatgtggt cctgtcaccg
     2281 gcgatcacgc cgccgtcggc gatctcgggc tcgttgttcg gcccggggct gagcgagatg
     2341 tagcggttcg cgatccctga gagggaggtc gacctaatca cggcggtcgt gccctcgtgg
     2401 aggggatcgt cggtggtgat cttgaccttc gcctgggcgt cctcggtgag cgagatctcg
     2461 tcgatcgtgc cgaccgcctg gccgccgacc aggacctggt tcccgggcac gagctggccg
     2521 ccggtctcga agaggagctc gtactcgtgg ccgtcgtcgt cgccgagaag cagcaccacc
     2581 acgagcacca ccgcgaccac cagcgcaccc agccccgcga tgcgcgccag gagccgtccc
     2641 gatcctccgc tatccggtcg cctgccctgc actcggggac ggcgtaagcc tcgctcctct
     2701 ctacccagtt gcttccgtgc ggtccagcga aaaagcgcaa tctttggctt gccgcaccgg
     2761 gcgctgcgcc cagggttgta cccgcgggca tcctaatcgc tggggcatat gccgagcggg
     2821 gcgtgtgttg cttaaccgag tcttagccga cccggcccca ggccggcgcc gacgcgctac
     2881 tcgcgcgggt cgcgaccggt gagtgcccgg tagaggtcgt tggcgcgcgt cccggtgagc
     2941 gccgcgacga ccgacgcggc ggcgcgggga cgcgcgccgg actgcaccag acgccgtagg
     3001 gcatcgaccg cgaaggccac gtcgggcccg gcgccggcgc tcgtcgcggg cccgatcacg
     3061 atgacgatct cgccctttgc ctcgccgcga tagcgccgcg cgagctcgcc cagcgtgccg
     3121 cgcgaaacct cctcgtgaag cttggtcagc tcccggcaga ccgccgccgg gcggtcgggc
     3181 gccagcgccg cgagcgccgc cagcgaggcg ggcagccggc gcggcgactc gaaggccacg
     3241 accgtctcgg gcgagccaag caccctttcg agctcgcctg cgcggcgcgg gagaaagccc
     3301 tcgaagcgcc accgatcggc cggcagcgcg gatgccacga gtgcggtggt gacggccgac
     3361 gggccgggca gcacctccac ctcgaggtcg cgctcgaggc aggcgcggat cagccggaag
     3421 ccggggtccg agatcaccgg ggtgcccgcg tcggagatca gcaccacccg ggcgccgcgc
     3481 tcgatctgct gggcgagctg gcgcgcgcgc tcggcctcgt tgccctcgta gttggagacg
     3541 agtcgcggcc gctcgagctc gagtcgctcg aacaggcggc cggcgcggcg ggtgtcctcg
     3601 caggcgacta tgtcggcctc gaccagcgcc cgtcgcgcgc gcgggctgag gtcgtcgacg
     3661 ttcccgatcg gcgtcgggca gaccgtgagc cggccgggca tcagacggtc acaccagcgg
     3721 cctcaacagc ggccgggctc ggcttcgcgc ctgcggcgct ccgcctcacg cggtctcctc
     3781 gagcgccatc tcggcagcgc cgatgaaccc cgagtcgggt cccatcgcgg ccgggaggac
     3841 cggggtctcg ttcatcggcg gcagcgcacg actgcgcagc tgctctcgtg ccggctcgag
     3901 cagcagatcg ccggcggcgg acactccgcc cccgatcacg atcacgtcgg ggtcgaagat
     3961 gttggcgagg ctggacagcg cgacgccgag acggcgcccg atcgtcgcca cgacctcgac
     4021 cgcgattcca tcgccgtcga tagcgtgctt cgtcaccagc cgcccggtga tctcctcgcc
     4081 ggccgcaagc gcacggccga gcgaagaccc gggatgggtc tcggcggcga tcctcccctc
     4141 ccgtccgagc gcggtgcccg acgccagcac ctcgacgcag ccgcggttgg ggcagttgcc
     4201 ctggcagggc gggcccgcct gatcgatgac cacgtggccg agctcggcgc ccgcgccggt
     4261 ggagccgcgg tagagctcgc cgccgagcac gagcccgccg ccgattccgg tgccgatcgt
     4321 gagcagcacg gcgttcgtgg cacccttcgc ggcaccgaac cggtgctcgg ccagcgccgc
     4381 caggttggcg tcgttgtcga ggaaggtagg aaggttgagc cgctgctcga cgagctctcg
     4441 gaacgggacg tcgacgat
//
LOCUS       DASRIG010000510         6764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2562863, whole genome shotgun sequence.
ACCESSION   DASRIG010000510 DASRIG010000000
VERSION     DASRIG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562863"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFB52_13600"
     CDS             <1..798
                     /locus_tag="VFB52_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HZO07418.1"
                     /translation="SAVSRWTLRVLGLGFLAMLLVAPLVIIFVETFSEGFMAPFDAIT
                     TENGLHALKLSLLMVVIAVPLNTIFGIVCAILLVRHKWKGNAVIDSVINLPFALSPIV
                     IGLSLYLLYSTNGGWFGPTLAEAGIKVLFSVPGMVLASIFVSLPFVVRETVPVLQEIG
                     TEQEQAASTLGANWWQTFWRVTLPAIRWGVAYGVVLTTARVLGEFGAVTIVSGAISGE
                     TETLPIFVQKQFDAFNNPGAYGASLLLALLALMTLLAMNLLKRKEDA"
     gene            798..1835
                     /locus_tag="VFB52_13605"
     CDS             798..1835
                     /locus_tag="VFB52_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HZO07419.1"
                     /translation="MGISVEGANKSFGDFQALNDVSIEVPDGSLTALLGPSGSGKSTL
                     LRAIAGLETLDSGNVLIDGEDVSSKPTQKREVGFVFQHYAAFKHMTVYENVGFGLKIR
                     KWDKQKINARVHELLDLVQLHGLAERYPSQLSGGQRQRMALARALAVEPQVLLLDEPF
                     GALDAKVRKDLRTWLRRLHDEMHVTTIFVTHDQEEAMDVAGQLVVMNEGRVEQSGTAD
                     ELYEQPANEFVMSFVGEVNRLGDDFVRPHDMEVVRDADPTTTEAMVQRIVALGFEIRV
                     EFVLGDGTEVWAQLTRGELERLELREGQIAYLRPVQAKSFNGEPASKHQGASTPPGST
                     DDGDAEVSAAV"
     gene            1992..2192
                     /locus_tag="VFB52_13610"
     CDS             1992..2192
                     /locus_tag="VFB52_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07420.1"
                     /translation="MSKRIDQTTFVSRGRITGLVRDEHPAGTGQNRTSRLANRDSGNA
                     ADEALFYRARLLLGDALPRPQR"
     gene            complement(2212..2874)
                     /locus_tag="VFB52_13615"
     CDS             complement(2212..2874)
                     /locus_tag="VFB52_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07421.1"
                     /translation="MKRRLSTPRAATVIVLAAVVAVSVTVVPSIAQSFLTPQRAAKVY
                     VSNKTAQSTYLKKKAASNLYVTKATAPKPTVGAVAAGTAQFGPISATTAGYIPSAFAS
                     FATKASVSNVAITLSGTGTCTAAKPGANLACPVEILIDGQKTGKVNFLPSTASSPTPV
                     PVVSTILKTTVLTKGGHTIAVQYAGAKDVTFTLKSWDLAVEAFPVPEEPVEEETGSKK
                     GE"
     gene            3037..3867
                     /locus_tag="VFB52_13620"
     CDS             3037..3867
                     /locus_tag="VFB52_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO07422.1"
                     /translation="MRIKSLAIAALALAGLAAPASASAVTYIGSGSVAAQPVLQALFK
                     TYETKVNKKVNFVYTANGGNAGVKDVQNGTSQFAGNARTPLPSDAGTTYIKMFMDGLC
                     LDVNTKNPLKNITIQQTHDIYRGLLTNWNQIPGSYADATISPVGRDTNGGTYNFFLQA
                     VLNNEPPASNVNALTADGLVVNAVKQDVNAIGYAGLAWLGSKKLKPLKVNGVPCQANK
                     ISVEPLKYPLSRYLFLVLPGDGSSSPKGLLKFVDWMRRSKLAGQVILKAGGVPAFNKK
                     "
     gene            3877..4869
                     /locus_tag="VFB52_13625"
     CDS             3877..4869
                     /locus_tag="VFB52_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZO07423.1"
                     /translation="MDEEFTPEYGPKWSDKRVERMLGALVVAVVGLLLALIITVFIKG
                     WPSFEHNGLAWFGSGGSVDDQLQDIFLSGQAGVDYVYTFHAWPLIWSTILITGGAILL
                     SLFSALFVAVFIVEFAPEWMSNILQPVVRFLASVPSVIYGLIGVVVLVPFIGNNLVTE
                     DQKASVAGVISLSGYSLIAAVIVLTVMIAPLMVSIFSDGLRSVKPGWLEGSLALGVNR
                     WRTTWKIGVRAARPAIVAGTVLATARAIGEAVMLAMISGSVGFAPNPLDGLIFFFEPS
                     RPLAPTIIKNIEALTTPPGSATLFSIATVLLLSAATASFAGFIARRSMRKYGSA"
     gene            4869..5873
                     /gene="pstA"
                     /locus_tag="VFB52_13630"
     CDS             4869..5873
                     /gene="pstA"
                     /locus_tag="VFB52_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZO07424.1"
                     /translation="MESSVATPPSPQPPSPPPSKGKATGAKEGSATWALSDRIGLAIC
                     WGLGLLFCAIAIAIVVYLLIQGITYLRPEMLWTPATAGFEQTESGGFSDALLGTLIVA
                     VMGSALAVPIGVAIAVWLTEYGRPAGLARVTESTIEAIAGIPSIVLALFGTVIFSSTA
                     LAFLSRSTEGVVFGRSFFAASAMLSLVALPLIVGSTREGLNAIPRHVREASYAVGKTK
                     AATTRRILIPTAKPQIATGAMLGLGRIIGDTAIIVVLLGATQNFAPVEGAPFPFDFLR
                     GAGTTLTNFVYEASPTGNLNQPEKAYAAAFVLLLMVLILNGAVDVIHRRARKVGTWSS
                     "
     gene            5861..>6764
                     /locus_tag="VFB52_13635"
     CDS             5861..>6764
                     /locus_tag="VFB52_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZO07425.1"
                     /translation="MEFLNQPPPPVRRLPLEDTPPAHEAAPPATSTNGHGNPAAVPAQ
                     QSGFTSAKRSISAPQFSIRRMEIDKVSVFYGEKEAVKSVSLPVRQGEVLSLIGPSGCG
                     KTTLLRSLNRLTELTSAAKLTGRILLDDIDIKAIEPTLLRRRVTMVFQQPNPFPMSVF
                     DNIAYVLREQGSRRARKKELEPAVHSALQRAGLFEEVKDNLKHPALKLSGGQQQRLCI
                     ARALAAEPEVLLLDEPCSALDPQSTQVIEDLIVKLREDVAVVIVTHNLQQAYRVADYV
                     GFMYLGELVEYSGAKQLFGKPKEDR"
BASE COUNT         1125 a         2385 c         2114 g         1140 t
ORIGIN      
        1 agcgccgtca gccgctggac cctgcgcgtc ctcggcctcg ggttcctcgc gatgctgctg
       61 gtcgcgccgc tggtgatcat cttcgtcgaa accttcagcg agggcttcat ggccccgttc
      121 gacgcgatca ccaccgagaa cggcctgcac gcgctcaaac tgagcctctt gatggtcgtg
      181 atcgcggtgc cactgaacac gatcttcggg atcgtctgcg cgatcctgct ggtccgccac
      241 aagtggaagg gcaacgcggt gatcgactcg gtgatcaacc tcccgttcgc cctctcgccg
      301 atcgtcatcg gcctctccct ctacctgctc tacagcacca acggaggctg gttcgggccg
      361 accctggcgg aagccgggat caaagtcctg ttctcggtcc cgggcatggt cctcgccagc
      421 atcttcgtct cgctgccctt cgtggtccgg gagacggtgc cggtgctgca ggagatcggc
      481 accgagcagg agcaggccgc ttcgacgctc ggcgccaact ggtggcagac cttctggcga
      541 gtcaccctgc cggcgatccg ctggggcgtc gcctacggcg tcgtgctcac caccgcccgc
      601 gtgctcggcg agttcggcgc ggtgacgatc gtctccgggg cgatttcggg agagaccgaa
      661 accctgccga tcttcgtcca gaagcagttc gacgccttca acaatccggg cgcctacggc
      721 gcctccctgc ttcttgccct gctcgcactg atgaccctgc ttgcaatgaa cctgctcaaa
      781 cgaaaggagg acgcctgatg ggaatctcag tcgagggagc caataagagc ttcggggact
      841 ttcaggcgct caatgacgta tcgatcgagg tcccggacgg gtccctgacc gccctgctcg
      901 gccccagcgg ctcgggcaag tccaccctgc tgcgggcgat tgccgggctc gagacgctcg
      961 attccggcaa cgtcctgatc gacggcgagg acgtctccag caagccgacc cagaagcgcg
     1021 aagtcggctt cgtcttccag cactacgccg ccttcaagca catgaccgtg tacgagaacg
     1081 tcggcttcgg gctgaagatc cgcaagtggg acaagcagaa gatcaacgcc cgcgtgcacg
     1141 agctgctcga cctcgtccag ctgcacggac tcgccgagcg ctacccctcc cagctctccg
     1201 gtggccagcg ccagcggatg gcgctggcgc gggcgctggc ggtggagccg caggtgctgc
     1261 tgctcgacga gcccttcggg gcgctcgacg cgaaggtgcg caaggacctg cgcacctggc
     1321 tgcgccgcct ccacgacgag atgcacgtga ccacgatctt cgtcacccac gaccaggagg
     1381 aggcgatgga cgtcgccggc cagctggtcg tgatgaacga gggccgggtc gagcagtcgg
     1441 ggaccgcgga cgagctctac gagcagcccg ccaatgagtt cgtgatgagc ttcgtcggcg
     1501 aggtcaaccg gctcggcgac gacttcgtcc gcccgcacga catggaggtc gtgcgcgacg
     1561 ccgatccgac cacgaccgag gcgatggtcc agcggatcgt cgccctcggc ttcgagatcc
     1621 gggtcgagtt cgtgctcggc gacgggaccg aggtctgggc gcagctgacc cgcggcgagc
     1681 tcgagcggct cgagcttcgg gaaggccaga tcgcctacct ccggccggtc caggcaaaaa
     1741 gcttcaacgg tgagccagcg tccaaacacc agggggcctc gactccccct gggtccacgg
     1801 acgacggcga cgcagaggtt tctgcagccg tctgaagagg cgacgggcgg gggcgcgctt
     1861 tttgccgggt ggcgcccccg cccacttcgc cgctttcttc agcgccgtaa gtttcccagg
     1921 cccttgcggt ctactcaatt actgctattc ttgagttaga tactcgacaa actccagaaa
     1981 ggacggcagg gatgagcaag cgaatagacc aaaccacgtt tgtctcgcgc ggtcggatca
     2041 ccgggctggt gcgtgacgag cacccggccg ggaccggcca gaaccggacc agccgtctcg
     2101 caaaccgtga ctccggcaac gcggccgacg aggcgctctt ctaccgcgct cgccttctcc
     2161 tcggcgacgc actcccccgc ccccagcgct agctgggggc ggggcgcgtt tctactcgcc
     2221 cttcttggag ccggtctctt cctcgaccgg ttcctcggga accgggaacg cctcgacggc
     2281 gaggtcccag cttttcagcg tgaaggtgac gtctttcgca cccgcatact gaaccgcgat
     2341 cgtgtgaccg cctttggtca gcaccgtcgt cttcaggatc gtgctgacga ccggcaccgg
     2401 cgtcggcgaa gacgcggtcg agggcaggaa attcactttg cccgttttct gaccgtcgat
     2461 caggatttcg accgggcagg ccaggttcgc accgggtttc gccgcggtgc aggtacccgt
     2521 gcccgagagc gtgatcgcca cgttgctgac cgaggccttg gtcgcgaacg aggcgaaggc
     2581 gctcgggatg tagccggcgg tggtcgcgga gatcgggccg aactgggccg tgccggcggc
     2641 aaccgcgccg accgtcggct tcggggccgt ggccttggtc acgtagaggt tcgaggccgc
     2701 tttcttcttc aggtaggtcg actgcgccgt cttgttgctg acgtagacct tggccgcccg
     2761 ctggggcgtc aggaacgact gcgcgatcga gggaacgacc gtcaccgaca cggcgacgac
     2821 ggccgccaga acgatgacgg ttgccgctcg gggcgtcgag agccttctct tcaacaaggt
     2881 gattcctcca tttccgggcc ccccacatcg gctcgatcgc tcctgccggt ggcctttatt
     2941 gacttactta gtcaataaac tacagaaact ggtagcttta cccaaccaag ttggtcaaga
     3001 aactcgaata cccggcaaag agaggaatca cagcagatga gaatcaagtc cctcgccatc
     3061 gcggctttgg cccttgcggg gctcgctgcg ccggcgtcgg ccagcgccgt gacctacata
     3121 gggtcgggct cggtggccgc acagccagtg cttcaggcgc tgttcaaaac gtacgaaacg
     3181 aaagtcaaca aaaaggtcaa tttcgtctac accgccaacg gcggcaacgc aggcgtcaaa
     3241 gacgtccaga acgggaccag ccagttcgct ggtaacgccc gtacccccct cccctcggac
     3301 gcgggtacga cctacatcaa gatgttcatg gacgggctct gccttgacgt caacaccaag
     3361 aacccgctca aaaacatcac gatccagcag acccacgaca tctaccgcgg gctgctgacc
     3421 aactggaacc agatccccgg gtcctacgcg gacgcgacga tctccccggt cggtcgtgac
     3481 accaacggag ggacctacaa cttcttcctc caggccgtgc tcaacaacga accgccggcc
     3541 tccaacgtga acgcgctgac cgccgacggc ctcgtcgtca acgcggtcaa gcaggacgtc
     3601 aacgcgatcg gctacgccgg tctcgcctgg ctcggcagca agaagctgaa acccctcaag
     3661 gtgaacggcg tcccctgcca ggccaacaag atcagcgtcg agccgctcaa gtacccgctc
     3721 tcgcgctacc tgttcctggt ccttccgggc gacggcagct cgagcccgaa aggcctgctc
     3781 aaattcgtcg actggatgcg ccgcagcaag ctcgctggcc aggtcatcct gaaagccggc
     3841 ggcgtccccg ccttcaacaa gaaataagcg ggaccggtgg acgaagagtt cactcctgag
     3901 tacggcccga aatggtcgga caagcgcgtc gagcgaatgc tcggcgcgct tgtcgttgcc
     3961 gtggtcgggc tgctgctggc cctgatcatc acggtcttca tcaaaggctg gccgtcgttc
     4021 gagcacaacg gcctcgcctg gttcggctcc ggcggcagcg tcgacgacca gctccaggac
     4081 atctttctct ccggacaggc cggcgtcgac tacgtctaca ccttccacgc ctggccgttg
     4141 atctggagca cgatcctgat caccggcggg gcgatcctgc tctcgctctt ctcggcgctc
     4201 ttcgtcgccg tgttcatcgt cgagttcgcg ccggaatgga tgagcaacat cctgcagccg
     4261 gtcgtccgct tcctggccag cgtgccctcg gtgatctacg gcctgatcgg cgtcgtcgtg
     4321 ctcgtcccct tcatcggcaa caacctcgtc accgaggacc agaaggcctc ggtcgccggc
     4381 gtgatctcgc tctccggcta cagcctgatc gccgcggtga tcgtgctcac ggtgatgatc
     4441 gcgccgctga tggtctcgat cttctccgac gggctgcgct cggttaaacc gggctggctg
     4501 gagggctcgc tggccctcgg cgtcaaccgc tggcgcacca cctggaagat cggcgtccgc
     4561 gccgcccggc cggcgatcgt cgccggcacc gtgctggcga ccgcgcgggc gatcggcgag
     4621 gcggtgatgc tggcgatgat ctccggctcg gtcggcttcg ccccgaatcc gctcgacggc
     4681 ctgatcttct tcttcgagcc ctcgcggccg ctcgccccga cgatcatcaa gaacatcgag
     4741 gcgctgacca cgccgcccgg cagcgcgacc ctgttctcga tcgccaccgt gctgctgctc
     4801 tcggccgcaa ccgcctcctt cgccggcttc atcgcccgcc gctcgatgcg caagtacggg
     4861 agtgcctgat ggagtcctcg gtagccaccc cgccgtcccc gcagccgccc tcgcctcccc
     4921 cctcgaaggg caaggcgacc ggcgccaagg agggcagcgc cacctgggcg ctctcggacc
     4981 ggatcggcct ggcgatctgc tggggcctcg gcctgctgtt ctgcgcgatc gcgatcgcga
     5041 tcgtcgtcta cctgctgatc caggggatca cctacctgcg cccggagatg ctctggaccc
     5101 cagccaccgc gggcttcgag cagaccgaaa gcggcggctt ctccgacgcc ctgctcggca
     5161 cgctgatcgt cgccgtgatg ggcagtgccc tggcggtgcc gatcggcgtc gcgatcgcgg
     5221 tctggctgac cgagtacggc cgccccgccg gcctagcccg ggtgaccgaa tcgacgatcg
     5281 aggcgatcgc cggcatcccc tcgatcgtgc tcgccctgtt cggcacggtg atcttctcca
     5341 gcaccgcgct cgccttcctc agccgcagca ccgaaggcgt cgtcttcggc cgctccttct
     5401 tcgccgcctc ggcgatgctc tcgctggtcg ccctgccgct gatcgtcggc tcgacccgcg
     5461 aggggctgaa cgcgatcccc cgccacgtgc gcgaggcctc ctacgcggtc ggcaagacca
     5521 aggcggcgac cacgcgccgg atcctgatcc cgaccgcgaa accgcagatc gcgaccgggg
     5581 cgatgctcgg cctcggccgg atcatcggcg acaccgcgat catcgtcgtg ctgctcggcg
     5641 cgacccagaa cttcgcgccg gtcgaaggcg ccccgttccc gttcgacttc ctgcgcgggg
     5701 ccggcaccac cctgaccaac ttcgtctacg aggcctcccc gaccgggaac ctcaaccagc
     5761 ccgagaaggc ctacgcggcg gccttcgtcc tgctgctgat ggtcctgatc ctcaacgggg
     5821 ccgtcgacgt catccaccgc cgcgcacgaa aggtagggac gtggagttcc tgaaccaacc
     5881 accgccgccg gtccgccggc tgccgctcga ggacacgccg cccgcccacg aggcggcgcc
     5941 gccggcgacg agcaccaacg gccacggcaa cccggccgcg gtgcccgcgc agcagagcgg
     6001 cttcacctcg gcgaaacgct cgatcagcgc cccgcagttc tcgatccggc ggatggagat
     6061 cgacaaggtc tcggtcttct acggcgagaa agaggcggtc aaatcggtct cgctgccggt
     6121 ccgccaaggc gaggtgctct cgctgatcgg cccctccggc tgcggcaaga cgacgttgct
     6181 gcgctcgctc aaccggctca ccgagctgac cagcgccgcc aaattgaccg gccgcatcct
     6241 gctcgacgac atcgacatca aggcgatcga gccgaccctg ctgcggcgcc gggtgacgat
     6301 ggtcttccag cagccgaacc cgttcccgat gagcgtcttc gacaacatcg cctacgtgct
     6361 gcgcgagcag ggttcgcggc gggcgcggaa gaaggagctc gagccggccg tccacagcgc
     6421 cctgcagcgg gccggcctct tcgaggaggt caaagacaac ctcaagcacc ccgcgctgaa
     6481 actctccggc ggccagcagc agcggctctg catcgcccgc gcgctcgccg ccgagcccga
     6541 ggtgctgctg ctcgacgagc cctgctcggc gctcgacccg cagtcgaccc aggtgatcga
     6601 ggacctgatc gtcaagctgc gcgaggacgt ggcggtggtg atcgtcactc acaacctcca
     6661 gcaggcctac cgggtggccg actatgtcgg cttcatgtac ctcggcgagc tggtcgagta
     6721 ctccggcgcg aagcagctct tcggcaagcc caaggaagac cgga
//
LOCUS       DASRIG010000511         8669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3103928, whole genome shotgun sequence.
ACCESSION   DASRIG010000511 DASRIG010000000
VERSION     DASRIG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103928"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFB52_13640"
     CDS             <1..354
                     /locus_tag="VFB52_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07426.1"
                     /translation="PVDFRYCRRESCAVYAGPHLTASNRRTTAFVEVDRRSRGWVELT
                     YWLYRPTLGWERVVRRASAGDVAAASGTKVLVSQDPALVPLETLPGRNHYEFAPHERP
                     PWQWYVRGHYPGWSS"
     gene            544..1116
                     /locus_tag="VFB52_13645"
     CDS             544..1116
                     /locus_tag="VFB52_13645"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HZO07427.1"
                     /translation="MAPAAAPAGWYPDTTRPGMQRYWDGAQWTNHMAPLAGVATPTAT
                     TTTTIGAPKQSRSIEVGPRLIVGAIGALIAFIAAFLPHAESDEVLTIANNTLMSNGDG
                     VFVVLLAIGGGVLAVVQSGKQGPSIALGIIGVITLLLGIFEGTDSALKVVNGLGQEVE
                     TTAGPAVWSLCLGGIVMTIAGFVPWRTSSQ"
     gene            complement(1169..2185)
                     /locus_tag="VFB52_13650"
     CDS             complement(1169..2185)
                     /locus_tag="VFB52_13650"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZO07428.1"
                     /translation="MPAADLQSSFEFFFDRAAEFFRNLSEIKWTAFGIALLCLLAMQI
                     ARAWAWRNVLKAAYPDKKIPFLPLAAAYLAGAGINAIIPAHAGDVTKVFLVKRQIPDS
                     SYPAVTSSFLVQTVFDTSVGILVLLYAITQGLLPPLPRIPDLPAFEISFWADNPQILA
                     IVVGVLVIATAVGIYFLAHRVRRFWDRVKQGVVILTQPRRYMREVFAWQGIGWLFRFA
                     AFWFFLEAFGIGGSIGNVMLVMSVQAIANIVPFTPGGAGAQQALLVATLHGPTRAAVL
                     SFSVGTQIAMAFWSIVLGFAAILLVFRTTDWRGLIQQAKDERDEEEDKAVSETAMLRP
                     QSPE"
     gene            2270..3196
                     /locus_tag="VFB52_13655"
     CDS             2270..3196
                     /locus_tag="VFB52_13655"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZO07429.1"
                     /translation="MCARARERFTADRKSRLRRRRKRHAAMPNLIIIGGLKCGTTSIH
                     HYLGLHPEIQMSKPKELNFFVEEMNWDLGLDWYASRFDGRSPVRGESSPHYTNLPRFE
                     GVAERIKENCPDARLLYMVRDPIKRILSHWVHATGAGYETREFEPTLALPGTQYVNRS
                     KYWMQLQPYLERFDREQIEIISQEELHADREGTMRKAFAFAGVDENFFSEQFDREWEK
                     SSAKQGDKYQFMEKLIKLPGFRSFDRNFDRLPESLRWIVEKVVHDPDKPPAEKPVLPD
                     ELFETLRGRFGEDVAELQKFAGREFAGWNDYS"
     gene            3218..3946
                     /locus_tag="VFB52_13660"
     CDS             3218..3946
                     /locus_tag="VFB52_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZO07430.1"
                     /translation="MRRLLPDPGETTVEQQLDGYRPWEHAHAERPLVAMNFAATVDGR
                     ATIGGVSGPIGSATDTEMLARLRTRFDAVMIGAGTMRAERYGRVVADQEQRERRERLG
                     LPHDPLMVIVSGRLDLPWDAPLFTNGGGRVLIFTAADEEPPETATPVRVVRHEGGVDL
                     AAALAHLRGERGIRALICEGGPRLHSQLQAGGLVDELFLTIAPKLTGAGPSILEGELP
                     DHPSLELAWLLEQEGELFARYRRA"
     gene            4000..5262
                     /locus_tag="VFB52_13665"
     CDS             4000..5262
                     /locus_tag="VFB52_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07431.1"
                     /translation="MDFKPSPEVDALRERVLDFIDDHVYPQEREIMEAMDAEVGPGVP
                     YSANLVAVREQAKADGLWNLFMPDERYGPGLTNWEYGLLCEEMGRSPLAAAMAFNCSA
                     PDTGNMEILAEHGTPEQVERWLDPLLEGTIRSCFSMTEPEVSGSDPTLLQASAVLDGE
                     EWVINGHKWFTSGAVGAELAIAMVVTDPDAAPYARASMICVPTDAPGFNLVRPIPVMG
                     HTAGPGHCEIRYEDCRVPAANLLGERGAGFAIAQDRLGPGRIHHCMRAIGTAERAHEM
                     MCRRANERVAFGGPLADKQFVQEAIATSRMEIDQARLLTLFAAWKMDTEGKRAARQQI
                     SAIKIVAANMVMEVLDRAIQIHGSLGMTDDTPLAALWRFSRMLRLADGPDEVHKMVLA
                     RRELNRWAKRDEAERAGTLEPRHATRMI"
     gene            5332..5571
                     /locus_tag="VFB52_13670"
     CDS             5332..5571
                     /locus_tag="VFB52_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZO07432.1"
                     /translation="MTEVGMHEAKTQLSRLVERALDGEEIVITRRGRAAVRLVPERPK
                     GGFASLQGVWKGKAKIADDFDELPDEIARAFGMID"
     gene            5574..5945
                     /locus_tag="VFB52_13675"
     CDS             5574..5945
                     /locus_tag="VFB52_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZO07433.1"
                     /translation="MLLDTHLIIWWMNGEASKISEKALAALGSEDMEPVVSAVAIWEA
                     AIKRRIGKLEAPGDMLAQIEGSGVELLPVTVRHADFVSSLPDHHADPFDRLLVSQAMI
                     EGIALASADEVLRRYDVELVW"
     gene            complement(5974..6684)
                     /locus_tag="VFB52_13680"
     CDS             complement(5974..6684)
                     /locus_tag="VFB52_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07434.1"
                     /translation="MSTIAVFLVLGGATAVAATQLAKNSVGTKQLKNNAVITAKLKNG
                     AVTAAKLGNGSVSSAKLSAGSVITEKLPNGAVTAAKLADASVSAAKLGSSSVTAGKID
                     AGAVTTDKILNSAVGTEKLAGEAVITGKLANGAVTSGKLGEGSVRASNLGVVTITTGP
                     FENAGSGATGEANLSCPAGTRMLSGGMLNPSNTKELVLIASYPKGANQWHVAVYNSAG
                     FVASYQVVVSCLQQVALP"
     gene            complement(6810..7505)
                     /locus_tag="VFB52_13685"
     CDS             complement(6810..7505)
                     /locus_tag="VFB52_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07435.1"
                     /translation="MKQIRKHLTLANVLSTVAVFLVLGGATAVAATQLGKNTVGTKQL
                     KSNAVTAAKIKKNAVTAAKIKANAITTAKVKNGAITGAKVNLGSLGTVPNSATTETVK
                     GSHGVLALGQEATVLEHGPLKLTVKCEVPEAAPTYLSPVAYISSSAAGSAFTSWDYGS
                     KELGPTTPESERKLNEYYWAGSTGSFGYDAPTDIGVSASAANGAAFNAFLGLAAEKDS
                     GTCWWWANATILG"
     gene            complement(7536..8231)
                     /locus_tag="VFB52_13690"
     CDS             complement(7536..8231)
                     /locus_tag="VFB52_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07436.1"
                     /translation="MKRIGKRITYANVMSSLAIFLVLAGGTAFAASQLAKNSVGAKQL
                     KSNAVTAAKIKKNAVTAAKIKANAITTAKVKNGAITGAKVNLGSLGTVPNSATTDTVK
                     SAKVVLTLGQEATLVERGPLKLIAKCETPEENPGYLSPRLYISSATAGSVFTSWEDGS
                     KELGPATPANERQLNEYTDGGSNGPWNYESPSEGGASASATNGSAFNAWVGVAAEKNT
                     GTCWYWASATILG"
     gene            complement(8309..>8669)
                     /locus_tag="VFB52_13695"
     CDS             complement(8309..>8669)
                     /locus_tag="VFB52_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07437.1"
                     /translation="GTSTYVQGADELNTYGGGLTFTFSPGCAAPRRAVTYLLVDVPNP
                     TKITETDITGYGEVIDPDGSKSTVRVDIGPFVFNSAKFEPGTPTTHTVTLITTSDCSA
                     GSGVTASAGAVDVIGTR"
BASE COUNT         1381 a         2905 c         2989 g         1394 t
ORIGIN      
        1 ccggtggact tccgatactg ccggcgcgag agctgcgcgg tctacgcggg gccgcacctg
       61 accgcgtcga accggcgcac gacggcgttc gtcgaggtgg accgacgctc gcggggctgg
      121 gtcgagctga cctattggct ctaccggccg accttgggct gggaacgggt ggtgcgccgg
      181 gcgagcgccg gtgacgtcgc ggcggcctcg gggaccaagg tcctcgtctc gcaggacccc
      241 gccctggtcc cgctcgagac cctgccgggt cgcaaccact acgagttcgc gccgcacgaa
      301 cgccccccgt ggcaatggta cgttcgcggg cactaccccg ggtggtcgag ctgaactggg
      361 cctgtcgact ttgttcctac aggggaaaca aagtcgacaa cggccgccgc gcgatcggat
      421 atacatcgca gtaccgcaag ctcgcactcg gtactcgtcc acgctttgac cgatcaaccg
      481 cctcctcccg ggaacgtccc gcccacgccg ccgccgcggc ctgtggctcc gccgccgcaa
      541 cccgtggcgc cggcggccgc gcccgctggc tggtacccgg acacgacccg gccggggatg
      601 cagcgctact gggacggcgc gcagtggacc aaccacatgg cgccgctcgc cggggtcgcc
      661 acccccacgg cgacgaccac caccacgatc ggcgcgccca agcagagccg ctcgatcgag
      721 gtcgggccgc ggctgatcgt cggcgcgatc ggcgccctga tcgccttcat cgccgccttc
      781 ctcccccacg ccgaaagcga cgaggtgctg acgatcgcca acaacacgct gatgtcgaac
      841 ggcgacgggg tctttgtcgt cctgctggcg atcggcggcg gtgtgctcgc cgtcgtccag
      901 tcgggcaagc agggcccgag catcgcgctc gggatcatcg gcgtcatcac cctgctgctg
      961 ggaatcttcg agggcaccga cagcgcgctg aaggtcgtca acggtctcgg ccaggaagtc
     1021 gaaacgaccg ccgggccggc cgtctggtcg ctctgcctcg gcgggatcgt gatgacgatc
     1081 gccggcttcg tgccctggcg cacgtcctct caataaggcg aggctcagag gccggcaggc
     1141 aaggacgcag ggagcccgaa tagcagcgct attcgggcga ctgaggacgc agcatggcgg
     1201 tctctgagac cgccttatct tcttcttcgt cgcgttcgtc cttcgcctgc tgaatcaggc
     1261 cgcgccagtc ggtcgtgcgg aagacgagca ggatcgcggc gaacccgagc acgatcgacc
     1321 agaacgccat cgcgatctgg gtgccgaccg agaaggagag caccgcggcc cgcgtcggtc
     1381 cgtgcagggt ggcgaccagc agcgcctgct gggcgccggc cccgcccggg gtgaagggga
     1441 cgatgttggc gatcgcctgc acgctcatca ccagcatcac gttgccgatc gagccgccga
     1501 tcccgaacgc ctcgaggaag aaccagaagg cggcgaagcg gaagagccag ccgatcccct
     1561 gccaggcgaa gacctcgcgc atgtagcggc gcggctgggt gaggatcacg acgccctgtt
     1621 tgacccggtc ccagaagcgg cgcacccggt gggcgaggaa gtagatcccg accgcggtcg
     1681 cgatcaccag gacgccgacg acgatcgcga ggatctgcgg gttgtcggcc cagaaggaga
     1741 tctcgaaggc gggcaggtcg gggatccgcg gcagcggcgg cagcagcccc tgggtgatcg
     1801 catagagcag gacgaggatg ccgacgctgg tgtcgaagac cgtctgcacc aggaacgagg
     1861 aggtcaccgc cgggtaggag gagtccggga tctggcgctt gaccaggaag accttggtca
     1921 cgtcgccggc gtgggcgggg atgatcgcgt tgatcccggc gccggccaga tacgcggccg
     1981 cgagcggcag gaaggggatc ttcttgtcgg ggtaggccgc cttcagcacg ttgcgccagg
     2041 cccaggcacg ggcgatctgc atcgcgagca ggcagagcag ggcgattccg aacgccgtcc
     2101 acttgatttc ggagaggttg cgaaagaatt ccgccgcccg gtcgaagaag aattcgaagc
     2161 tcgactgaag atcggcggcg ggcatcgcag ctacactagc ccgctcgtgc cggataccga
     2221 cacgcacacc ttgccccgcc cagccacccc gcacccgacc gatgaggcca tgtgcgcgcg
     2281 tgcccgcgag cgcttcacgg cggatcgcaa gagccgcctg cgccggcgcc gcaaacgcca
     2341 cgcggcgatg ccgaacctga tcatcatcgg cggcctcaag tgcggcacga cgagcatcca
     2401 tcactacctc ggcctgcatc cggaaatcca gatgtcgaag ccgaaggagc tgaacttctt
     2461 cgtcgaggag atgaactggg acctcggcct cgactggtac gcgagccgct tcgacgggcg
     2521 ctcgccggtg cgcggcgagt catcgcccca ctacaccaac ctgccccgct tcgagggcgt
     2581 cgccgagcgg atcaaagaga actgccccga cgcgcgcctg ctctacatgg tgcgcgaccc
     2641 gatcaagcgg atcctctcgc actgggtgca cgcgaccggc gccggctacg agacacgtga
     2701 gttcgaaccg accctcgccc tgccgggaac ccagtacgtc aaccgctcca agtactggat
     2761 gcagctgcag ccctacctcg agcgcttcga ccgcgagcag atcgagatca tcagccagga
     2821 ggagctgcac gccgaccgcg agggcacgat gcgcaaggcg ttcgccttcg ccggcgtcga
     2881 cgagaacttc ttctccgagc agttcgaccg cgagtgggag aagtcgagcg ccaagcaggg
     2941 cgacaaatac cagttcatgg agaagctgat caagctgccg ggctttcgct ctttcgaccg
     3001 caacttcgac cgcctgcccg agtcgctgcg ctggatcgtc gagaaggtcg tgcacgaccc
     3061 cgacaagccg ccggccgaga agccggtcct ccccgacgag ctgttcgaga cgctgcgcgg
     3121 ccgcttcggc gaggacgtcg ccgaactgca gaagttcgcc ggccgcgagt tcgccggctg
     3181 gaacgactac agctgagacc cgggtagcct gctgcgaatg cggcggctgc ttccagatcc
     3241 cggggagacc accgtcgagc agcagctcga cggctaccgg ccgtgggagc acgcgcacgc
     3301 cgagcggccg ctggtggcga tgaacttcgc cgccacggtc gacgggcggg cgacgatcgg
     3361 cggcgtctcc ggaccgatcg gctccgccac cgacaccgaa atgctggccc gactgcggac
     3421 ccgcttcgac gcggtgatga tcggcgccgg gacgatgcgg gccgagcgct acgggcgcgt
     3481 cgtcgccgac caggagcagc gcgaacggcg cgagcggctc ggcctccccc acgacccgct
     3541 gatggtgatc gtcagcggcc ggctcgacct gccctgggac gcgccgctgt tcaccaacgg
     3601 cggcggcagg gtgctgatct tcaccgccgc cgacgaggag ccaccggaga ccgcgacccc
     3661 ggtgcgagtg gtccgccacg agggcggcgt cgacctcgcc gcggcgctgg cccacctgcg
     3721 cggagagcgc ggcatccggg cgctgatctg cgagggcggc ccgcgcctgc actcgcagct
     3781 gcaggccggc ggcctcgtcg acgagctgtt cctgacgatc gcgccgaaac tgaccggtgc
     3841 cgggccctcg atcctcgagg gcgaactgcc cgatcacccg agcctggagt tggcctggct
     3901 gctcgagcag gagggcgagc tgttcgcccg ctaccgccgc gcctgactgg ctggtcaact
     3961 agcgttgcgt cgagcaagcg acgagggagg acgacgtaga tggacttcaa gcccagccca
     4021 gaggttgacg cgctgcggga gcgcgtactc gacttcatcg acgaccatgt ctacccgcag
     4081 gagcgcgaga tcatggaggc gatggacgcc gaggtcgggc ccggcgtgcc ctactcggcc
     4141 aacctcgtcg cggtccgcga gcaggccaag gccgacggcc tctggaacct gttcatgccc
     4201 gacgagcgct acgggccggg gctgaccaac tgggagtacg ggctgctctg cgaggagatg
     4261 ggccgcagcc cactggccgc ggcgatggcc ttcaactgct cggccccgga caccggcaac
     4321 atggagatcc tcgccgagca cggtaccccc gagcaggtcg agcgctggct cgatccgctg
     4381 ctcgagggca cgatccgctc ctgcttctcg atgaccgagc ccgaggtctc cggctcggac
     4441 ccgaccctgc tgcaggcaag cgcggtgctc gacggcgagg agtgggtgat caacggccac
     4501 aagtggttca ccagcggtgc ggtcggcgcc gagctggcga tcgcgatggt cgtcaccgat
     4561 cccgacgccg ccccctacgc gcgcgcctcg atgatctgcg tgccgaccga cgcgccgggc
     4621 ttcaacctgg tccggccgat ccccgtgatg ggccacaccg ccggccccgg ccactgcgag
     4681 atccgctacg aagactgccg cgtgccggcc gccaacctgc tcggcgagcg cggcgccggc
     4741 ttcgcgatcg cccaggaccg gctcggtccg ggccgcatcc accactgcat gcgcgcgatc
     4801 gggaccgccg agcgcgccca cgagatgatg tgccgccgcg ccaacgagcg cgtcgccttc
     4861 ggcgggccgc tggccgacaa gcagttcgtc caggaggcga tcgcgacctc gcggatggaa
     4921 atcgaccagg cccggctgct cacccttttc gccgcctgga aaatggacac cgagggcaag
     4981 cgcgccgccc gccagcagat ctcggcgatc aagatcgtcg ccgcgaacat ggtgatggag
     5041 gtgctcgacc gggcgatcca gatccacggc agcctcggga tgaccgacga cacgccgctg
     5101 gcggcgctct ggcgtttcag ccgcatgctg cggctcgccg acggccccga cgaggtccac
     5161 aagatggtcc tcgcccgccg cgagctgaac cgctgggcga agcgcgacga ggccgagcgc
     5221 gccgggaccc tcgagccccg gcatgccacg cgcatgatct gagcgcccac gttcggctcg
     5281 cttcgggcgc tatctagcta gatatctagc tagctctggt agattcggcg cgtgaccgag
     5341 gtggggatgc acgaagccaa gacccagcta tcgcggctgg tcgagcgggc gctcgacggc
     5401 gaggagatcg tgatcacgcg gcgcggccgt gccgccgtcc ggctcgtgcc ggagcgaccg
     5461 aagggtggat tcgcctcgct gcaaggcgtg tggaagggta aggcgaaaat cgccgacgac
     5521 ttcgacgagc tgcccgacga gatcgccagg gcattcggga tgatcgattg aggttgctgc
     5581 tcgacaccca cctgatcatc tggtggatga acggcgaagc gagcaagatc agcgagaagg
     5641 ccctggccgc gctcggctcc gaggacatgg aaccggtggt cagcgccgtc gcgatctggg
     5701 aggcggcgat caagcgccgc atcggaaagc tcgaagctcc cggcgacatg cttgcccaga
     5761 tcgagggctc gggggtcgag ctcctgccgg tcaccgtgcg ccatgccgac ttcgtctcct
     5821 ccctgcccga ccaccacgcg gacccgttcg atcgcctgct ggtctcacag gcgatgatcg
     5881 agggcattgc cctggcgagc gcggacgagg tgctgcggcg ctacgacgtc gagctcgtct
     5941 ggtgagcggc acgccacgcg gatggtctag ctcctagggc agtgccacct gctgcagaca
     6001 ggagacgacg acctggtagc tagcgacgaa cccggcgctg ttgtagacgg cgacgtgcca
     6061 ctgattggcg cctttcgggt aggaggcgat cagcacgagc tctttggtgt tgctggggtt
     6121 caacatgccg ccgctcagca tcctggttcc agccggacag ctgaggttcg cttctccggt
     6181 ggcaccagat cccgcgttct cgaagggtcc ggtggtgatg gtcacgaccc cgaggttcga
     6241 ggccctgacc gagccttcgc ctagtttgcc gctggtcacc gcgccgttgg cgagcttgcc
     6301 ggtgatcacc gcttccccgg ccagcttttc ggtgccgacg gcgctgttga ggatcttgtc
     6361 cgtggtcacc gcaccggcat cgatcttgcc ggcggtcacc gagctggagc cgagcttggc
     6421 ggcagagacc gaggcgtcgg cgagcttggc agcggtcacc gcaccgttcg ggagcttttc
     6481 ggtgatcacc gagccggcgc tcagtttcgc gctgctgacc gagccgttgc cgagcttggc
     6541 cgcggtcacg gcgccgttct tcagcttcgc cgtgatcacg gcgttgttct tcagctgctt
     6601 ggtgccgacc gagttcttgg cgagctgcgt ggcggccacc gcggtggcgc cgccgagcac
     6661 gaggaagacg gcgatcgtcg acatcacgtt tgcgtacgtg agccgcttac ttaggtgctt
     6721 cacggaagtt cctttcgttt gtggccgaaa cgcggagggc cgaggcatac gccccggccc
     6781 tccggaaagc gacgaatctc aggctagggc tagccgagga tggtcgcgtt cgcccaccac
     6841 caacaggtgc cgctgtcttt ctcggcggcg aggccgagga aggcgttgaa agcagcaccg
     6901 ttcgccgcgc tggcgctgac gccgatatcg gtcggcgcgt cgtagccgaa ggagcccgtg
     6961 ctccccgccc agtagtactc gttcagcttc cgttcggact cgggggtggt aggaccgagt
     7021 tccttgctgc cgtagtccca gctggtaaag gccgagccgg cggcactgga cgagatgtag
     7081 gcgaccggcg agaggtaggt cggcgccgct tcgggaactt cgcatttgac cgtcagcttc
     7141 aacggcccgt gttccagcac cgtggcctcc tggccgagcg cgagcacacc gtggcttcct
     7201 ttcaccgttt cggtcgtggc cgagttcggc accgtgccga gcgagccgag gttgaccttg
     7261 gcgccggtga tcgcgccgtt cttgaccttc gccgtggtga tcgcgttggc cttgatcttg
     7321 gccgcggtca ccgcgttctt cttgatcttc gcggcggtca ccgcgtttga tttcagctgc
     7381 ttcgtgccaa ccgtgttctt gcccagctgg gtggcggcga ccgcggtcgc accaccgagc
     7441 acgaggaaga cagcgacggt ggaaagcacg ttcgcgagcg tcaggtgctt gcgtatctgc
     7501 ttcacgtcaa tctcctttcc ggagctgctg cctcgctagc caaggatcgt cgcgctggcc
     7561 cagtaccagc aggtgccggt gtttttctcg gccgccacgc ctacccaggc gttgaacgcg
     7621 gacccgttgg tggcgctggc gctggccccg ccttcgctgg gcgattcgta gttccagggg
     7681 ccgttgctgc cgccatcggt gtattcgttg agctgccgct cgttcgcggg agtggccgga
     7741 ccgagttctt tgctgccgtc ctcccagctg gtgaagaccg agccggcggt ggccgaggag
     7801 atgtagaggc gcggcgagag gtagccgggg ttctcttccg gcgtctcgca ctttgcgatc
     7861 agcttcagcg ggccgcgttc gaccagcgtc gcttcttggc cgagggtgag gaccactttc
     7921 gcgcttttca ccgtgtcggt cgtcgccgaa ttcggcaccg tgccgagcga gccgaggttg
     7981 accttggcgc cggtgatcgc gccgttcttg accttcgccg tggtgatcgc gttggccttg
     8041 atcttggcgg ccgtgaccgc gttcttcttg atcttcgcgg cggtgaccgc gttgctcttc
     8101 agctgcttgg cgccgaccga gttcttggcg agctgggagg cggcgaaggc cgtgccaccg
     8161 gcgaggacga ggaagattgc tagcgaggac atcacgttgg cgtacgtgat ccgcttaccg
     8221 atgcgcttca agcgaagctc ctttggattg gggggaacaa gcggccggga ccctagtcag
     8281 agccccggcc gcctttcccc ccgcggagct agcgagtgcc gattacgtcg actgccccgg
     8341 ccgaggcggt gacgccgctg ccggcggagc aatcggacgt ggtgatcagg gtcacggtgt
     8401 gggtggtcgg cgtgccgggc tcgaacttgg ccgagttgaa gacgaacggg ccgatgtcca
     8461 cgcgcaccgt cgatttggag ccgtccgggt cgatcacttc cccgtaaccg gtgatgtcgg
     8521 tctcggtgat tttggtcggg ttcgggacgt cgacgaggag gtaggtcacg gcccgtcgcg
     8581 gcgccgcgca gccgggggag aaggtgaacg tcagaccgcc gccgtaggtg ttcagttcgt
     8641 cggctccctg aacgtaggtc gaggtcccc
//
LOCUS       DASRIG010000512         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3905592, whole genome shotgun sequence.
ACCESSION   DASRIG010000512 DASRIG010000000
VERSION     DASRIG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905592"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFB52_13700"
     CDS             <1..708
                     /locus_tag="VFB52_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07438.1"
                     /translation="DGSVVTVPPAFTKDAVFEAILNGIKLVVIVTERIPRGDVAQMVE
                     LANMEGARIIGPNCLGLIVPEVCKMGGIGGPAKDAAKAYAPGPVGVMSRSGGMTTEIS
                     SSLTQAGLGVSTAVSIGGDAIIGSAYAELMPYFEADEETQAIVIYTEPGGRMEAQLAE
                     WVAENNSRLPIIAFMAGKFMDDEEMKGMSFGHAGTIVEGKEDTATEKIARLEAAGIRV
                     VERIDEIPSAVKEKLAA"
     gene            708..953
                     /locus_tag="VFB52_13705"
     CDS             708..953
                     /locus_tag="VFB52_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZO07439.1"
                     /translation="MAEQMNSVFIEVTVDEPTASDPALGAKLEEVCPVSIFKGTDSGV
                     DVVEENTDECVLCKLCLEAAPAGTVHVKKLYDGTELS"
     gene            1039..1635
                     /locus_tag="VFB52_13710"
     CDS             1039..1635
                     /locus_tag="VFB52_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07440.1"
                     /translation="MKRISLVIVLAAALLTALVATSQAEVEATPASVDLGAIDYGQQP
                     LGVKVNFKLTADETHRQFVSLGLGGDAAAFKFVDDCAAMLGGGSDNCTAYVAVAETAS
                     PGLHQGTLSLVLKDPGGAQITKTVGLSVTVGPAPQPPPSSPDRTHKDKDRGRGPDKGS
                     SKGKAKGKKGCGKGKAKAKGAKGKKRCGKAKGKKGHRA"
     gene            2035..2688
                     /locus_tag="VFB52_13715"
     CDS             2035..2688
                     /locus_tag="VFB52_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO07441.1"
                     /translation="MDASVRTRLSRDDRMEQTLNVAHGLFAERGYADVKMDEIAAAVG
                     VTKPLLYNYFGNKERLYIACMERAGDALEKTVGEAVEATKSPGDALGAGVHAFFSFLD
                     SDRAAWAVLFDETLPQAGEVAERVAEYRGNILAFVSASMLAQLPSGKRAGAKVEIEAL
                     STALLGAAEALARWWLRTEAVSADEAAELLIATIEPGLRQRSQLRTSPPRSKGKTTA"
     gene            2685..3980
                     /locus_tag="VFB52_13720"
     CDS             2685..3980
                     /locus_tag="VFB52_13720"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZO07442.1"
                     /translation="MNPQTRRVAIVGGNRIPFARSDGPYAHASNRDMLTAALDGLVDR
                     YGLEGETLGEVAGGAVLKHSHDRDLTRESVLSTRLAPETPGYDVQQACGTGLETTILV
                     ANKIALGQIDAGIACGADTTSDAPIALNEQLRSTLLEANRQRSTTGKLRALTGFRPQH
                     VIPAIPRNEEPRTGLSMGEHCAIMASEWEIGRADQDELAVASHHKLAAAYERGFFDDL
                     LTPYLGLERDQNLRPDSSTEKLAKLKPVFGGEEGTMTAANSTPLSDGASAVLLASEEW
                     AKERDLPVLAYLCEAQTAAVDHVHKREGLLMAPAYAMPKMLDRAGVTLQDFDYYEIHE
                     AFAAQVLCTLAAWEDEAYCKDKLGRDKPLGAIDRGKLNVNGGSLAAAHPFAATGGRIV
                     AGLAKQLHEDGTGRGVISICAAGGQGVVAILEGPGGATK"
     gene            3977..>4741
                     /locus_tag="VFB52_13725"
     CDS             3977..>4741
                     /locus_tag="VFB52_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07443.1"
                     /translation="MSDRYSQLVNAPVVSTVAKQLGLPQPVELDRYRVGAPVVSGPVL
                     SGAAPSGRLQKEIAALLDEIKAERAGGEGKAKALIFDATGIGDSTELVELQRFFYPAV
                     GRLARSGRVVVLGTPPAAAGSPRAHTAQRALEGFVRSLGKEIGGRGSTAQLVLVEPGG
                     EDQLGSTLRFLLSPRSAYVSGQVVRIEAGVAPLPALDWTRPLAGQTALVTGASRGIGA
                     AIAETLARDGADVIGLDIPPAAEDLRKLTDKLGGEAV"
BASE COUNT          805 a         1758 c         1562 g          616 t
ORIGIN      
        1 gacggctcgg tcgtcaccgt cccccccgcc ttcaccaagg acgcggtctt cgaggcgatc
       61 ctcaacggga tcaagctggt cgtcatcgtc accgagcgga tcccgcgcgg cgacgtcgcc
      121 cagatggtcg agctggcgaa catggagggg gcgcggatca tcggccccaa ctgcctcggc
      181 ctgatcgtcc ccgaggtctg caagatgggc ggcatcggcg gccccgccaa ggacgcggcc
      241 aaggcctacg cgcccggacc cgtcggcgtg atgagccgct ccggcgggat gaccaccgag
      301 atctcctcct cgctgaccca ggccggcctc ggcgtctcga ccgcggtctc gatcggcggc
      361 gacgcgatca tcggctccgc ctacgctgag ctgatgccct acttcgaggc cgacgaggag
      421 acccaggcga tcgtgatcta caccgagccc ggcggccgca tggaggccca gctggccgag
      481 tgggtggccg agaacaacag ccgcctgccg atcatcgcct tcatggccgg caaattcatg
      541 gacgacgagg agatgaaagg catgtccttc ggccacgccg gcacgatcgt cgagggcaag
      601 gaggacacgg ccacggagaa gatcgcccgg ctcgaagcgg ccggcatccg cgtggtcgag
      661 cggatcgacg agatcccgtc tgcggtgaag gagaaactgg cggcgtaatg gcggagcaga
      721 tgaacagcgt cttcatcgag gtcacggtcg acgagccgac cgcctccgac cccgccctcg
      781 gcgccaagct cgaggaagtc tgccccgtca gcatcttcaa gggcaccgac tccggggtcg
      841 acgtggtcga ggagaacacc gacgagtgcg tgctctgcaa gctctgcctc gaagccgccc
      901 ccgccggcac cgtgcacgtc aagaagctct acgacggcac cgagctttcc tagcccgctc
      961 cgggcacctg ggtaggtctt acgcagcgcc cgtcgcggcc cgttgatcgg cccgtgcgag
     1021 cgcaatagct tgccgcgcat gaagcggatc tcgctcgtga tcgtcctcgc cgcggcgctg
     1081 ctgaccgcgc ttgtcgcgac cagccaggca gaagtcgaag cgacgccggc ctcggtcgac
     1141 ctcggcgcga tcgactacgg gcagcagccc ctcggcgtaa aggtcaattt caaactgacc
     1201 gcggacgaga cccaccgcca gttcgtcagc ctcggcctcg gcggcgacgc cgccgcgttc
     1261 aaattcgtcg acgactgcgc cgcgatgctc ggcggcggct ccgacaactg caccgcctac
     1321 gtcgccgtcg cggagaccgc cagccccggt ctccaccagg gcacgctctc gctcgtgctc
     1381 aaagaccccg gaggcgccca gatcaccaaa acggtcggcc tcagcgtcac cgtcggtccc
     1441 gctcctcagc ctccgccctc cagccccgac cgcacccaca aagacaaaga ccgcgggcgc
     1501 ggtcccgaca agggatcgag caagggcaaa gcgaaaggca aaaaaggctg cggcaaagga
     1561 aaagcgaaag ccaaaggcgc caagggcaag aagcgctgcg gcaaagccaa aggcaaaaag
     1621 ggccaccgcg cctgaatcac gaagacccgc cgaagcgggt cttcgtgcgg gggagctacc
     1681 cccggaacgg ctgataaccg cgagacagca gattagcgcg acctagccgt ccgccttcgg
     1741 ggggcgaatc aagggccggc ctgccttatg ccagtcggag gatgaagcgg cgaggcgccg
     1801 caaacggttg ataaccgcga gtccggagta gccctccgga tggggttcga gtcccccgtc
     1861 ctccgcagtg tttcgccgag aacgcggatc ggacgagggt gcaatagatc aggcgcccgc
     1921 gagtagcgct cgcccagtcg tcaccccgcg cttctcggca gacatccacc tagatgcctg
     1981 cgcggcgcat cagttacttg catataaatt tatatctggg taacgtgtac accgatggat
     2041 gcatcggttc gcacccgcct ctcgcgcgac gaccgcatgg agcagacgct gaacgtcgcc
     2101 cacgggctgt tcgccgagcg cggctacgcc gacgtgaaga tggacgagat cgcggcggcg
     2161 gtcggggtga ccaagccgct cctctacaac tacttcggca acaaggagcg gctctacatc
     2221 gcctgcatgg agcgggcggg cgacgcgctc gaaaagacgg tcggcgaggc cgtcgaagcg
     2281 accaagtcgc ccggcgacgc gctcggggcc ggcgtccacg ccttcttctc cttcctcgac
     2341 agcgaccgcg ccgcctgggc ggtgctcttc gacgagaccc tgccccaggc cggcgaggtc
     2401 gccgagcggg tcgccgagta ccgcggcaac atcctcgcct tcgtctccgc ctcgatgctg
     2461 gcgcagctgc cgagcggcaa gcgcgccggc gccaaggtcg aaatcgaggc cctctccacc
     2521 gccctgctcg gcgccgccga ggcactcgcc cgctggtggc tgcgcactga ggcggtcagc
     2581 gccgacgagg cggccgagct gctgatcgcc acgatcgagc ccggcctgcg ccagcgctcc
     2641 cagcttcgta cctccccacc ccgaagcaaa ggaaagacca ccgcatgaat ccccagactc
     2701 gccgcgtcgc gatcgtcggc ggcaaccgca tccccttcgc ccgctccgac ggcccctacg
     2761 cccacgcctc caaccgggac atgctgaccg cggcgctcga cgggctggtc gaccgctacg
     2821 gactcgaggg cgagaccctc ggcgaggtcg ccggcggcgc ggtgctcaag cactcccacg
     2881 accgcgacct cacccgcgag agcgtgctca gcacccggct ggcccccgag accccgggct
     2941 acgacgtcca gcaggcctgc gggaccggcc tggagacgac gatcctggtc gccaacaaga
     3001 tcgccctcgg ccagatcgac gccggcatcg cctgcggtgc cgacaccacc tccgacgcgc
     3061 cgatcgcgct caacgagcag ttgcgttcga ccctgctgga ggccaaccgc cagcgctcga
     3121 cgaccggcaa gctgcgcgcc ctgaccgggt tccgccctca gcacgtgatc ccggcgatcc
     3181 cgcgcaacga ggagccgcgc accgggctct cgatgggcga gcactgcgcg atcatggcca
     3241 gcgagtggga gatcggccgc gccgatcagg acgagctcgc ggtcgccagc caccacaaac
     3301 tcgccgccgc ctacgagcgc ggcttcttcg acgacctgct caccccctac ctcgggctcg
     3361 agcgcgacca gaacctgcgc ccggactctt cgaccgagaa gctggcgaag ctgaagccgg
     3421 tcttcggcgg cgaggaaggg acgatgaccg cggccaactc gacgccgctc tccgacggcg
     3481 cctcggcggt gctgctcgcc tccgaggagt gggcgaaaga gcgcgacctg ccggtcctcg
     3541 cctacctgtg cgaggcccag accgccgcag tcgaccacgt ccacaagcgc gaagggctgc
     3601 taatggcgcc cgcctacgcg atgccgaaga tgctcgaccg ggccggggtc acgctccagg
     3661 acttcgacta ctacgagatc cacgaggcct tcgccgccca ggtgctctgc accctcgccg
     3721 cctgggagga cgaggcctac tgcaaggaca agctcggccg cgacaaaccg ctcggcgcga
     3781 tcgaccgcgg caagctcaac gtcaacggcg gctcgctggc cgccgcgcac ccgttcgccg
     3841 cgaccggcgg gcggatcgtc gccgggctcg ccaagcaact gcacgaagac ggcaccggcc
     3901 gtggcgtgat cagcatctgc gccgccggcg gccagggcgt cgtcgccatc ctcgagggcc
     3961 cgggaggtgc caccaaatga gcgatcgcta ctcgcagctc gtcaacgcgc cggtcgtctc
     4021 gaccgtcgcc aagcagctcg gcctgccgca gccggtcgag ctcgaccgct accgcgtcgg
     4081 cgccccggtc gtcagcggcc cggtgctgag cggcgccgcc cccagcggcc gcctgcagaa
     4141 ggagatcgcc gcgctgctcg acgagatcaa ggccgaacgg gccggcggcg agggcaaggc
     4201 gaaggcgctg atcttcgacg cgaccgggat cggcgactcg accgagctgg tcgagctgca
     4261 gcgcttcttc tacccggcgg tcggccgcct ggcgcgcagc ggccgcgtgg tcgtgctcgg
     4321 caccccgccc gccgccgccg gctcaccccg ggcccacacc gcgcagcggg cgctggaggg
     4381 cttcgtgcgc tcgctcggca aggagatcgg cggccgcggc tcgaccgcgc agctcgtcct
     4441 ggtcgagccc ggcggcgagg accagctcgg ctccacgctg cgcttcctgc tctcgccgcg
     4501 ctcggcctac gtctccggcc aggtggtccg gatcgaggcc ggggtcgcgc ccctgccggc
     4561 gcttgactgg actcggccgc tggccggcca gaccgcgctg gtcaccggcg cctcgcgcgg
     4621 gatcggcgcc gcgatcgccg agaccctcgc ccgtgacggc gccgacgtga tcgggctcga
     4681 catcccgcct gccgccgagg acctgcgcaa gctcaccgac aagctcggag gcgaggccgt
     4741 c
//
LOCUS       DASRIG010000513         9207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2049403, whole genome shotgun sequence.
ACCESSION   DASRIG010000513 DASRIG010000000
VERSION     DASRIG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049403"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFB52_13730"
     CDS             complement(<1..274)
                     /locus_tag="VFB52_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07444.1"
                     /translation="MADPVMLITGASSGIGAATARAAAPTHRLVLAARRQEQLEALAE
                     ELGGPERALAVRCDVTEWDQVEAMTAAALEGFGRIDAVFATAGFGAK"
     gene            375..776
                     /locus_tag="VFB52_13735"
     CDS             375..776
                     /locus_tag="VFB52_13735"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZO07445.1"
                     /translation="MDPQRKRKIRLVTALSVAVLLAVALIYTSFSASSPAKEPSELLN
                     ATPGTSYEMTGRVVEGSVKHDGDGISFEVKDRDGSSEPLPVTYSGTIPDPFRGGREII
                     LTGSVESGTFVGEPDTLITKCPSKFTTESNA"
     gene            769..3225
                     /locus_tag="VFB52_13740"
     CDS             769..3225
                     /locus_tag="VFB52_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZO07446.1"
                     /translation="MPSLGSALLALAFLTAVSAGVVALLGTRGERRWIDLSRRLVSAF
                     FGLLLTCVVIIEIAFATNDFSFDLVVEHSSIETPMGYKLAAMWSSQEGSLLLWAFVLS
                     IAASLALYATRNKLRDLVPWATAVMMGIAAFFTGMMLFGDGVNPFAKAEPGVVDGVGL
                     NPLLQHPSMMIHPPMLYSGYVAFTVPFAFAIGALITRRLDAEWIRSTRKFALVAWAFL
                     GLGLLLGARWSYTELGWGGYWAWDPVENAALMPWLLGTAFLHSIMVQEKRGMLKVWNA
                     SLIVATFSMALLGTFLVRSGVLQSIHAFGDSTVGPYILGLIGVVVIGSTVLIVSRLDD
                     LKSEKRIDSLLSRESVFLVNNLLLIAIVLVTFWGTFFPLISELFTGEKASLAAPWFDE
                     YTTPLGVALVLFTGIGPLLAWRRVSWDSAKRIFLVPVAVAAVVGVALALFTDAADSFW
                     ALALFSFAAFAVTGIAQEFWRGAAARHRLSGESPLAALVGIVTRNRRRYGGYIVHVGV
                     IVLLIGIAASSSFQTKEDVELRPGESAVVDGKQITYVEPTVKVTDQYLQFGTHLRVSE
                     GGKVLYDLFPNRRYFRPTGQTTGEISDFFEGESTSEVGLKAGPGEDFWTAAQPDLGGV
                     KQQVRAADEAFKTCVRGGPGAPPQCEALTELMQAAALNPKLQPAALAQIQELQQLTAT
                     KIARSYLDDTGAATIRVIVDPLVTWMWIGGLIALAGALIALWPSRGRRRGALVHTEAD
                     ARKEAKYREIRDAELDHATGKLSDEDFAVVDAELRREAVEILDDVGAGKAGNGGNGAR
                     ANGGANGSGAAKTPEKAERG"
     gene            3238..3462
                     /gene="secG"
                     /locus_tag="VFB52_13745"
     CDS             3238..3462
                     /gene="secG"
                     /locus_tag="VFB52_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZO07447.1"
                     /translation="MYTFLSVIQVILSVALIFLVLLHSGKDAGLSGAFGVGGGGSSIG
                     GGSMVERNLDRATIACAILFVLNTVVLLKI"
     gene            3635..4861
                     /locus_tag="VFB52_13750"
     CDS             3635..4861
                     /locus_tag="VFB52_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HZO07448.1"
                     /translation="MEATTADDHGHHHHHHHDEVEVSPATWTDGKRYAWLLGILIPVA
                     PFIAWGIDALTGFGAIYFIGPVLVFIAFPILDIVVGLDNTNPPDSVLKYLEQDRYYRW
                     CTYVFIPIQYAGLVLACYLWSSGDLNIVENIGLALTMGVVGGIAINTAHELGHKRASA
                     EKWLSRVALAQTGYGHFFIEHNRGHHVRVATPEDPASSRLGESFWAFLPRTVAGSLSS
                     AWGIECARLDRMGKSQWTLRNDILGAWAMTVGLFAVLAIVFGPVVLPYLLVQAVIGFS
                     LLEVVNYLEHYGLLRQKREDGRYERCLPEHSWNSNNVASNVLLYHLQRHSDHHANPTR
                     RYQALRHMDEAPQLPTGYAGMIVLAWIPPLWRRVMDRRLLEHFDGDVGRANIHPPKRR
                     QVLARYGDAPEGAANG"
     gene            4854..5030
                     /locus_tag="VFB52_13755"
     CDS             4854..5030
                     /locus_tag="VFB52_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZO07449.1"
                     /translation="MASAWRCPGCGYVFDEAEGHPREGFPAGTPWSEVPEDWACPDCG
                     VRDKVDFEPVGEDV"
     gene            5057..5662
                     /locus_tag="VFB52_13760"
     CDS             5057..5662
                     /locus_tag="VFB52_13760"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZO07450.1"
                     /translation="MSSARTPYAEAARELLRQTLFGAARDQLEQRPWSEVTMSDIAAT
                     AGVSRQTLYKEFGNRNEFGLAFVINEGEHFLDEVDQAVIANQADPRAAVAAALELFLV
                     TASDDPLIKMLLSDDGTGGMLPFVTTQGMPVVQWATARLATTIEAGWPQAPKADVLLL
                     SEALVRLAISYITAPSESPETIAASVADLLGPFIDRALGGS"
     gene            5861..6217
                     /locus_tag="VFB52_13765"
     CDS             5861..6217
                     /locus_tag="VFB52_13765"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HZO07451.1"
                     /translation="MDSHPQESAPRGELAAQLSREIVQLHARLYGRGPSKARSYLHTD
                     YAACVLEEIFTTAERTLIDAGSPEHVQNTRKKFQDAVKGEFVDIVERVTGRKVRVFLS
                     QVDVEANLALEFFLFD"
     gene            complement(6214..6567)
                     /locus_tag="VFB52_13770"
     CDS             complement(6214..6567)
                     /locus_tag="VFB52_13770"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HZO07452.1"
                     /translation="MATKQTAKGGQALTEISNAMVALHREHFGRGPGAAKTILDGDVV
                     VCVLTDVYTQVEKTLIEAGQVEHVRETRALHQTAIEEQYKRRVEEITGRRVNAFLSVV
                     HVDPDVSVETFLLDP"
     gene            6707..7885
                     /locus_tag="VFB52_13775"
     CDS             6707..7885
                     /locus_tag="VFB52_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO07453.1"
                     /translation="MFSRPSSKTVLLAVALAAFLGLLGGAALAADLQEELDTKVDKLD
                     QVEERQGVLTSELERMGNQISRLQIEVTNLRDAEAAVEAELAAKQAELDTAEADLESA
                     VDHLDVVRDRLKRALVALRERLIAIYEAGSPDLASIVLGSEDLSDAASRAEYVTRIQE
                     HDDALVDRVGELRDEAQSNVERQRELRDQIASARDQIAAHEAELQSTRVSLESQQGEL
                     ISARGARKQTLAKLDERAETLEGDVSSLQAEIQSQLGGLPSGSYPAGPTAPGTFIFPV
                     SGTVTSPFGYRWGRMHEGIDIGAAEGTPIWAADDGTVVLQQSEAESGGYGNYTCLDHG
                     GGLATCYAHQSAFNVSPGDSVSQGEVIGLVGNTGNSFGAHLHFEVRINGVAYDPMGYL
                     "
     regulatory      7942..8097
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            8148..8939
                     /locus_tag="VFB52_13780"
     CDS             8148..8939
                     /locus_tag="VFB52_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZO07454.1"
                     /translation="MAAALAGCLGIWAADANAAAAGGGLAPSGTPRLSDATCMSRCVS
                     ARKATPGATVRLTGSYLDETSRVVFKGVSKPVVATYKSRTSSTVEVVVPAEATSGYPR
                     VVSETGLRSNASPKLLEVVPASALPKQVFPVRGPHEYWAGFGDGRGHEGADIGAACGT
                     PLVAVAAGTLTKSEYHPRAGNYLVIDLKRTDQDLAYMHMTELTTLKVGTPVSAGQVVG
                     FTGDSGNASGCHLHFELWEGEYYGGGAPVDPIPFLKSLEPRAKRK"
     gene            complement(8957..>9207)
                     /locus_tag="VFB52_13785"
     CDS             complement(8957..>9207)
                     /locus_tag="VFB52_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZO07455.1"
                     /translation="LRRGLAAVEGTPGVRDVRVLGAIGVVELDHPVDVAAASAAAIER
                     GVWLRPFGNLVYAMPPYVCSADEIAAISAAMRDAAAAG"
BASE COUNT         1421 a         3300 c         3035 g         1451 t
ORIGIN      
        1 gcttggcgcc gaagccggcg gtggcgaaga ccgcgtcgat ccggccgaag ccctccagcg
       61 cggcggcggt catcgcctcg acctggtccc actcggtgac gtcgcagcgc accgccagcg
      121 cccgctcggg accgcccagc tcctcggcca gcgcctccag ctgctcctgc cgccgcgccg
      181 ccaggacgag gcggtgggtg ggggcggcgg cgcgcgcggt tgcggcgccg atgccgctgc
      241 tcgctccggt gatcagcatg actgggtcgg ccacagccaa gagtctgcca ggtgcggtcg
      301 cacttgtttc cgcataccgg caacaactgc gaccgcaggt cgcccgacgg ccgggattgc
      361 gataattggg actcgtggac ccccagcgca agcggaagat ccgcctcgtc accgcgctca
      421 gcgtcgccgt cctcctggca gtggcgctga tctacacctc cttttccgcc tcatcgccgg
      481 cgaaggagcc ctcggagctg ctcaacgcca cccctggcac cagctacgag atgaccggcc
      541 gcgtggtcga gggctcggtc aaacacgacg gcgacgggat cagcttcgag gtcaaggacc
      601 gcgacggcag cagcgagccg ctgccggtca cctacagcgg cacgatcccg gatccgttcc
      661 ggggcggtcg cgagatcatc ctcaccggca gcgtcgagtc gggcactttc gtcggcgagc
      721 cggacaccct gattaccaag tgcccgtcga agttcaccac cgagagcaat gcctagcctt
      781 ggcagcgctc tgctcgcgct cgccttcctg accgccgtct cggccggcgt cgtcgcgctg
      841 ctcggcaccc gcggcgagcg ccgctggatc gacctctcgc gccgcctcgt ctccgccttc
      901 ttcggcctgc tcctcacctg cgtggtgatc atcgagatcg ccttcgccac caacgacttc
      961 tccttcgacc tggtcgtcga gcactcctcg atcgagacgc cgatgggtta caagctggcg
     1021 gcgatgtggt cgagccagga gggctcgctg ctgctttggg ccttcgtgct ctcgatcgcc
     1081 gccagcctcg ccctctacgc gacccgcaac aagctgcgcg atctggtccc ctgggcgacc
     1141 gcggtgatga tgggaatcgc cgctttcttc accgggatga tgctgttcgg cgacggcgtc
     1201 aacccgttcg cgaaggccga accgggggtg gtcgacgggg tcggcctgaa cccgctgctg
     1261 cagcacccga gcatgatgat ccacccgccg atgctctact cggggtacgt cgccttcacc
     1321 gtgcccttcg ccttcgcgat cggggccctg atcacccgcc ggctcgacgc cgagtggatc
     1381 cgctcgaccc gcaaattcgc cctcgtcgcc tgggctttcc tcgggctcgg cctgctgctc
     1441 ggtgcccgct ggtcctacac cgagctcggc tggggcggct actgggcctg ggacccggtc
     1501 gagaacgcgg cgctgatgcc gtggctgctc ggcaccgcct tcctgcactc gatcatggtc
     1561 caggagaagc gggggatgct gaaggtctgg aacgcgagcc tgatcgtcgc caccttctcg
     1621 atggcgctgc tcggcacctt cctcgtccgc tccggcgtcc tgcagtcgat ccacgccttc
     1681 ggcgacagca ccgtcggccc ctacatcctc ggcctgatcg gcgtggtggt gatcggctcg
     1741 accgtgctga tcgtctcccg gctcgacgac ctcaagtccg agaagcggat cgactcgttg
     1801 ctctcgcgcg agtcggtctt cctggtcaac aatctgctgc tgatcgcgat cgtcctcgtc
     1861 accttctggg gcaccttctt cccgctgatc tcggaactgt tcaccggcga gaaggcctcg
     1921 ctggcggcgc cctggttcga cgagtacacg acgccgctgg gggtcgcgct ggtcctgttc
     1981 accggaatcg ggccgctgct cgcctggcgc cgggtcagct gggactcggc gaaacggatc
     2041 ttcctcgtcc cggtcgcggt cgccgccgtt gtcggggtcg cgctcgccct cttcaccgac
     2101 gccgccgact cgttctgggc gcttgccctg ttcagcttcg ccgccttcgc cgtgaccggg
     2161 atcgcgcagg agttctggcg tggggcggcc gcccgtcacc gtctctccgg cgaatcgccg
     2221 ctcgccgccc tggtcgggat cgtcacccgc aaccggcgtc gctacggcgg ctacatcgtc
     2281 cacgtcggcg tgatcgtgct gctgatcggg atcgccgcct cctcgagctt ccagaccaaa
     2341 gaggacgtcg agctgcggcc gggggagagt gccgtggtcg acggcaagca gatcacctac
     2401 gtcgagccca ccgtcaaggt caccgaccag tacctgcagt tcggcacgca cctgcgggtc
     2461 agcgagggcg gcaaggtgct ctacgacctt ttcccgaacc gccgctactt ccgcccgacc
     2521 ggccagacga ccggggaaat ctccgacttc ttcgagggcg agtcgaccag cgaggtcggg
     2581 ctcaaggcag ggccgggaga ggacttctgg accgccgccc agccagacct ggggggggta
     2641 aaacagcagg tgcgggcggc cgacgaggcg ttcaaaacct gcgtgcgcgg tgggcccggg
     2701 gcgccgccgc agtgcgaggc gctaaccgaa ctgatgcagg ccgccgcgct caacccgaaa
     2761 ctgcagccgg cggcgctggc gcagatccag gagctgcagc agctgaccgc gaccaaaatc
     2821 gcccgcagct atctcgacga caccggcgcg gcgacgatcc gggtgatcgt cgacccgctg
     2881 gtcacctgga tgtggatcgg cggcctgatc gccctcgccg gggcgctgat cgcgctctgg
     2941 ccgagtcgcg gccggcgccg tggcgcgctc gtgcacaccg aagccgacgc ccgcaaggaa
     3001 gccaagtacc gcgagatccg cgacgccgag ctcgaccacg cgaccggcaa gctctccgac
     3061 gaggacttcg ccgtggtcga tgccgagctg cgccgcgaag cggtcgagat cctcgacgac
     3121 gtcggcgccg gcaaggcggg caacggcggc aacggggcga gggccaacgg gggcgcgaac
     3181 ggcagcggcg cggccaagac ccccgaaaag gccgagcgcg gctagacttc ccggaccgtg
     3241 tacacgtttc tcagcgtcat ccaggtaatt ctttccgtcg ccctgatttt cttggtgctc
     3301 ctgcactcgg gcaaagacgc gggcctctcc ggcgctttcg gcgtcggcgg tggcggcagc
     3361 tcgatcggcg gcggctcgat ggtcgagcgc aacctcgatc gcgccaccat cgcctgcgcc
     3421 attctcttcg tgctgaacac cgttgttctc ctgaagatct agcgcccgcc tggcgcttcc
     3481 ccctgccttt attcgcccag aggagcgaat aagagcgtgt gcctgcacct acctgggcgt
     3541 catttcgcgc tgctttgtat tgccggttga caaaactggc cagtttgtct agcttgacga
     3601 cattcagtca acttctgaca gggaggaggc caaagtggag gccactactg cagacgatca
     3661 cgggcatcac catcaccacc atcacgacga ggtcgaggtc agccccgcga cctggactga
     3721 cggcaagcgc tacgcctggc tgctcgggat cctgatcccg gttgcgccgt tcatcgcctg
     3781 gggcatcgac gcgctgaccg gtttcggcgc gatctacttc atcggtccgg ttctcgtttt
     3841 catcgccttc ccgatcctcg acatcgtggt cgggctcgac aacacgaacc cgccggacag
     3901 cgtcctcaag tacctcgagc aggaccgcta ctaccgctgg tgcacctacg tcttcatccc
     3961 gatccagtac gccgggctgg tgctcgcctg ctacctctgg tcgagcggcg acctcaacat
     4021 cgtcgaaaac atcggcctgg ccctgaccat gggcgtggtc ggcgggatcg cgataaacac
     4081 cgcccacgag ctcggccata agcgggccag cgctgagaag tggctctccc gggtcgcgct
     4141 cgcgcagacc ggctacggcc acttcttcat cgagcacaac cgcggccacc acgtgcgcgt
     4201 cgccaccccc gaggacccgg ccagctcgcg gctgggggag agcttctggg ccttcctgcc
     4261 gcgcaccgtc gccggcagcc tcagctcggc ctgggggatc gagtgcgcgc gcctggaccg
     4321 catgggcaag tcgcagtgga cgctgcgcaa cgacatcctc ggcgcctggg cgatgaccgt
     4381 cggcctcttc gccgtgctgg cgatcgtctt cgggccggtc gtcctgccct acctgctggt
     4441 ccaggcggtg atcgggttct cgctgctgga ggtcgtcaac tacctcgagc actacgggct
     4501 gctgcgccag aagcgcgagg acgggcgcta cgagcgctgc ctccccgagc acagctggaa
     4561 cagcaacaac gtcgcctcca acgtcctgct ctaccacctg cagcgccact ccgaccacca
     4621 cgccaacccg acccgccgct accaggcgct ccgccacatg gacgaagcgc cgcagctgcc
     4681 gaccggctac gcggggatga tcgtgctcgc ctggatcccg ccgctctggc gacgggtgat
     4741 ggaccggcgc ctgctcgaac acttcgatgg cgacgttggc cgcgccaaca tccacccgcc
     4801 gaagcgacgc caggtgctcg cccgctacgg cgatgctccc gagggggcgg cgaatggcta
     4861 gcgcctggcg ttgccccggg tgcggctacg tcttcgacga ggccgaggga catccccgcg
     4921 agggcttccc cgccgggacg ccgtggagcg aggttcccga ggactgggcc tgccccgatt
     4981 gcggggtgcg cgacaaggtc gacttcgagc ccgtcggaga ggacgtttga ccgctcccgc
     5041 tgcgaccatt ggggtcgtgt cgtccgcccg taccccttac gccgaggccg ctcgcgagct
     5101 gctgcgccag acgctgttcg gcgccgctcg cgaccagctg gagcagcggc cctggtcgga
     5161 ggtgacgatg tccgacatcg ccgccacggc cggggtcagc cgccagaccc tctataagga
     5221 gttcggaaac cgcaacgagt tcgggctcgc cttcgtgatc aacgagggcg agcacttcct
     5281 cgacgaggtc gaccaggcgg tgatcgccaa ccaggccgat ccgcgggccg cggtcgcggc
     5341 ggcgctggag ctgtttctgg tcaccgccag cgacgacccg ctgatcaaga tgctgctctc
     5401 cgacgacggc accggcggca tgctgccgtt cgtcaccacc cagggcatgc cggtcgtgca
     5461 gtgggcgacc gcgcggctgg cgacgacgat cgaggccggc tggccgcagg cgccgaaagc
     5521 cgacgtcctg ctgctctccg aagccctggt gcggctggcg atcagctaca tcaccgcgcc
     5581 cagcgagtcg ccggagacga tcgccgcgtc ggtcgccgac ctgctcgggc cgttcatcga
     5641 cagggcgctc ggcggatcct gagaaatcgg ggactcccgc tatgctctcc gggctcggat
     5701 gccctggcgg cggcgggcgt agtccaggta tgcggcacag ctcgcgtgca ggggtgcttg
     5761 gcaggaccga agccagggtg aacggctccg cggcggaaag cgggcgcaag gtctttgcgt
     5821 acccctcgcc tgatcaacag gcagcaaagg agccgcacgc ttggattccc atccacagga
     5881 gagtgccccg cggggcgagt tggccgcgca gctctcgcgc gagatcgtgc agctgcacgc
     5941 gcgcctctac gggcgcggcc cgagcaaggc gcgcagctat ctgcacaccg actacgcggc
     6001 ctgcgtgctc gaggaaatct tcaccaccgc cgagcgcacg ctgatcgacg ccggctctcc
     6061 ggagcacgtc cagaacaccc gcaagaagtt ccaggacgcg gtcaagggcg agttcgtcga
     6121 catcgtcgag cgggtcaccg gccgcaaggt ccgcgtcttc ctcagccagg tcgacgtcga
     6181 ggccaacctc gcgctcgagt tcttcctctt cgactagggg tcgagcagga aggtttcgac
     6241 cgagacgtcg gggtcgacgt ggacgacgct gaggaaggcg ttgacccggc ggccggtgat
     6301 ctcttccacc cgccgcttgt actgctcctc gatcgctgtc tggtgcaggg cgcgggtctc
     6361 gcgcacgtgc tcgacctggc cggcttcgat cagcgtcttc tcgacctggg tgtagacgtc
     6421 ggtgagcacg cagacgacga cgtcgccgtc gaggatggtc ttcgctgcac cgggaccgcg
     6481 gccgaagtgc tcgcggtgca gggcgaccat cgcgttggag atctcggtga gtgcctgtcc
     6541 gcctttggcg gtctgctttg tggccatctt cggcgtttga atcggctcac gggcagttta
     6601 ggttgagcgc aaggaggccg aaaccctggc tgcaagcctg tgttcagggg ggtcgtgagg
     6661 ggccggcttt cggggccggc gccaatacct tcactggtcc tcagcgatgt tctcgcgccc
     6721 gagcagcaaa accgtcctcc tcgcggtggc cctggccgcc tttctcggcc tgctcggcgg
     6781 cgccgcgctg gccgccgacc tgcaggaaga actcgacacc aaggtcgaca agctcgacca
     6841 ggtcgaagaa cgccaggggg tgctgaccag cgagctggag cggatgggca accagatctc
     6901 ccgcctgcag atcgaagtca ccaacctgcg tgacgccgag gcggcggtgg aagcagaact
     6961 ggcggccaag caggcggagc tcgacacggc cgaagccgac ctggaaagtg ccgtcgacca
     7021 tctcgacgtc gtccgcgacc ggctgaaacg ggcgctggtc gcgctgcgcg aacggttgat
     7081 cgcgatctac gaggccggct cgcccgacct cgcctcgatc gtgctcggct ccgaggacct
     7141 ctccgacgct gccagccggg ccgaatacgt gacccggatc caggaacacg acgacgcgct
     7201 cgtcgaccgg gtcggcgaac tgcgcgacga agcgcagtcg aacgtcgagc gccagcgtga
     7261 gctgcgcgac cagatcgcct ccgcccgtga ccagatcgcc gcccacgagg ccgaactgca
     7321 gagcacccgc gtcagcctcg agtcccagca gggcgaactg atcagcgccc gcggcgcccg
     7381 caaacagacg ctggccaagc tcgacgaacg ggccgaaacc ctggagggcg acgtctcctc
     7441 gctgcaggcc gaaatccagt cccagctcgg cggcctgccc tcgggcagct acccggccgg
     7501 gccgaccgct cccggcacct tcatcttccc ggtcagcggc accgtcacct cgcccttcgg
     7561 ataccgctgg ggccgcatgc acgagggcat cgacatcggc gccgccgaag gaacgccgat
     7621 ctgggcggcc gacgacggca ccgtcgtgct ccagcagagc gaggccgaaa gcggcggcta
     7681 cggcaactac acctgcctcg accacggcgg cgggctggcg acctgttacg cccaccagtc
     7741 ggccttcaac gtctcgcccg gcgactcggt cagccagggc gaggtgatcg gcctggtcgg
     7801 caacaccggc aactcgttcg gcgcccacct gcacttcgag gtccgcatca acggggtcgc
     7861 ctacgacccg atgggctacc tctagcgcgc ctgggagggc cgtttcgcgc gctgccgcca
     7921 ggcgggtgcc tatactgcgc agcgacctaa ctgcctaatt cccgggtgtc tcacgcccgg
     7981 ggagcggggg aaccatgggg gagacatccc caggggcgaa tcggacgcac agtccgtagc
     8041 gcgactcttt cagcgcgagc ccgacagcta accccgccgg ccgacgagag aggaacgcat
     8101 tggtcggtac aacttcttgg cgaacgcgcg ggagccgtgt cctgctggtg gcggctgccc
     8161 tggccggctg cctggggatc tgggcggccg acgcgaacgc cgccgcggcc ggcggtggcc
     8221 tcgcccccag cggcaccccg cggctcagcg acgccacctg tatgagccgc tgcgtcagtg
     8281 cccgcaaggc gaccccgggt gccaccgttc gcctcaccgg cagctacctc gacgagacca
     8341 gccgcgtggt cttcaaaggg gtcagcaagc cggtcgtcgc gacctacaaa agccggacca
     8401 gcagcacggt cgaagttgtc gtgccggcgg aagccacgag cggctatccg cgcgtggtca
     8461 gcgaaaccgg cctgcgctcc aacgcctcgc ccaaactgct cgaggtcgtc cccgccagcg
     8521 cgctgcccaa acaggtcttt ccggttcgtg gcccgcacga gtactgggcc gggttcggcg
     8581 acggtcgcgg ccacgagggt gccgacattg gcgccgcttg cgggaccccg ctggtcgccg
     8641 tcgccgccgg caccctgacc aaaagcgagt accacccgcg cgccggcaac tacctggtga
     8701 tcgacctcaa acgcaccgac caggacctcg cctacatgca catgaccgaa ctgaccacgc
     8761 tcaaggtcgg cactccggtc agcgccggcc aggtcgtcgg cttcaccggt gactccggca
     8821 acgcctccgg ttgtcacctc cacttcgagc tctgggaagg cgagtactac ggcggcggcg
     8881 cccccgtcga cccgatcccc ttcctcaaat cgctcgagcc gcgggccaag cgcaagtagc
     8941 gctctggtag ccgctctcag ccggcggccg cggcgtcccg catcgccgcg ctgatcgcgg
     9001 cgatctcgtc ggcgctgcag acgtagggcg gcatcgcgta gacgaggttg ccgaacgggc
     9061 gcagccagac gccccgctcg atcgccgcgg cgctggccgc ggcgacgtcg acggggtggt
     9121 cgagctcgac caccccgatg gccccgagca cgcgcacgtc gcgcacacca ggggtgccct
     9181 cgacggcggc gagcccccgc cgcaggc
//
LOCUS       DASRIG010000514         7377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3105533, whole genome shotgun sequence.
ACCESSION   DASRIG010000514 DASRIG010000000
VERSION     DASRIG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105533"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFB52_13790"
     CDS             complement(<1..267)
                     /locus_tag="VFB52_13790"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HZO07456.1"
                     /translation="MTSSTSNGSSADAFVGVDVGGTHTDVSVVVGNRVERGKALTTYD
                     DFSRGVLEAVDVAAGRFDLSVEELLGKTKLFINGTTVVTNTITTL"
     gene            complement(354..1937)
                     /locus_tag="VFB52_13795"
     CDS             complement(354..1937)
                     /locus_tag="VFB52_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO07457.1"
                     /translation="MDDPNRSPANRWNSLGLPYLFERGGLDLEKKAVVFGDRSRTYGE
                     LRDRSRRVAGGLIGIGVEPGDRVALLSSNSLEYLEIEVGISAARGVMVPLNWRLRSGE
                     LANLLRRSGAQTIFVEDRFLHTVAELRRTGEVPELRTIIALGEGAGDLGYEELCASVS
                     GQHPGRPARMDDLHEIIFTSGTTGEPKGVVWSDGTVLWNSIQQAMDFQLGPQHASYAI
                     IDLYYIGGRHDFTWPILHQGGTVHIKPSSNFDAEETVAYVAEHGVTHILWVPTMLYEI
                     LRLPNLAEYDLSRLKMIMCGGQPLSADTAERAAAAFPATDFIQVYGLTEGGGSVTFLP
                     PAKAAEKAGSAGLPSQHVELRVVDEDGEDVAPGVDGEIVIRAPSVTPGYWADPELTDR
                     LIVNGFLHTGDMGRFDEDGFLFISGRKDDMIISGGMNVFPSEIEDVLRTHAEVDDVAV
                     IGLPDRKWGEVVCAVVQLEPGAATEEQALIDYCYERLAGFKKPSSVVVVEEIPRTAGG
                     KPKKFVLREQMRESSASLR"
     gene            2142..2531
                     /locus_tag="VFB52_13800"
     CDS             2142..2531
                     /locus_tag="VFB52_13800"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZO07458.1"
                     /translation="MATSFDPLIAADLSEPQRSTLQSFATRLREALGEDLLALWLYGS
                     RARGEGVHPESDVDLMVIAKGGRERYQRVAWDLSEEAAISEGESPFSYSVHVHDREWL
                     SRRREIESFFIQEVDRDKIVITGSALE"
     gene            2552..2902
                     /locus_tag="VFB52_13805"
     CDS             2552..2902
                     /locus_tag="VFB52_13805"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZO07459.1"
                     /translation="MEQARERLADAQAISDAVRPALVVSAAYYSMLYAARAALSERGE
                     FAKTHSGTWTLFSQHFVATGEFDQALSAMARRAKDAREQGDYEASPPSAAEATEFVDG
                     AAAFIAATERLLDD"
     gene            complement(2909..4966)
                     /locus_tag="VFB52_13810"
     CDS             complement(2909..4966)
                     /locus_tag="VFB52_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZO07460.1"
                     /translation="MEEERSDLGELAAAAQRALGSDAVVIARTEVERAPTGERLLAVA
                     GVDGLTGRNLDDLRRGLTHLDSALDFGDREWIGIPFLAGTRIAGANIVLSHGFASVLV
                     APVVVEGRRLGAVYAFKREPRSFAREGLVATFARQAAIAMDQDRSRLHLEVRGQSLAA
                     LDELVLAAHNFAELCRAIEKAVTPLFDAEKTGIMVWDERREVLQMVGGSFGASEEVAL
                     SCQIPVLDPHSNSARVFTTGRAYYTNDARNDPGIMQEYVNAFGIEKLLSTPLTLAGRP
                     IGVLHIANPGTDFGVEDLDRAEGLTARIAGAVELARTLFRLRRQQRLEEILSQVAVAV
                     ASGESVTDFLNPALKGVGRAVEASFLAMVPTEGEPAIWRHGASPLEDVVLEEVRRKPG
                     MRAYVVGPQKAGDPGWAVFYAPVHLGRQRVGTLAALRTRGEPFARDERRAIVRLANLA
                     ALSFATERYQQQRAELARLQERQRIADDLHDDVAQILFAAQLNLDAILEREPAESTIA
                     AGVERALGLLVRGDTEIRKVIHRLSNPASPDFDQRLAAAIVGVEQEFSVAIHTQFSDA
                     AIAVANRLRKPPADALIKAAREALVNAAKHAGPCRVSVQLSLTDRERLLLTVMDDGVG
                     APRSNSEPGHGLASLRRTLDEHGGCLRVGRAPAGGTKVTASIPIPASDPVSAEDPVPT
                     VKV"
     gene            complement(4987..5661)
                     /locus_tag="VFB52_13815"
     CDS             complement(4987..5661)
                     /locus_tag="VFB52_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO07461.1"
                     /translation="MQKRLLIVDDHEVVREGLASALATDERFEIVGTAASGKEALERA
                     RQTLPDIALVDLRLPDMRGEDLTREIRTRFPSTAVIVLSTYLGEGTVRASLDAGAAAY
                     VTKAAGLPELRAAIDRVIADDGAVDGTAAPQIVKQLHSLVAARMDDSIPTPRQEAVLE
                     LAAQGLTNGEIGDRLFISESTVRFHVQKLKEKFSAKTKTELIAKAIRTGFIAPALEDG
                     VPQAEL"
     gene            complement(5767..6804)
                     /locus_tag="VFB52_13820"
     CDS             complement(5767..6804)
                     /locus_tag="VFB52_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZO07462.1"
                     /translation="MSAEAAIDPAKAAAALELLRSVDAAGTRAEVSSLTQLEGGWSRH
                     TYMLDVADPDQAAPLELIVRVRPADSVLDTDLGQEFAIYEAIEDEPVPSPRVHGYQPA
                     DETAFGGPFFLMDKLPGKAVNVWRQRDRDELTENWEGSRTLAEDFVDRMAAIHLVDPA
                     KLAGAAVARDFRQTVARWRAIYEEVHLVEDPVVEEAYAWLLENEPDPVQPRLVHGDYR
                     IGNCLIDKGRLSGVLDWELASIGDPRFDLGYLSLDYHGGRFVTPGSPLCGAVAERGWM
                     EERYEAATGQPVDRSVVNVFAAMGALMLFSIMGTGLHLYSKGESSDIRAAWSRFVFPG
                     LRADLASLMGW"
     gene            complement(6801..>7377)
                     /locus_tag="VFB52_13825"
     CDS             complement(6801..>7377)
                     /locus_tag="VFB52_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07463.1"
                     /translation="DDAACRHWIATHGLAGSNHYPLRPRAAPSSACVAPVHASNPHQP
                     PGGLLMFNALRTDELIAGMGVTMRAAAAADGPADEYARGQLLSAFSISRLLAAEVRAT
                     PGLLAWLKGELDAALAASEDPAAASARERVAEAGDADAIGAALVDLLAELRAEGRDPE
                     LRKRVHAVLREMAARELAALAARPERRGESK"
BASE COUNT         1057 a         2533 c         2633 g         1154 t
ORIGIN      
        1 caaggtggtg atcgtgttgg taacgacggt ggtgccgttg atgaagagct tggtcttgcc
       61 cagcagctcc tcgaccgaga ggtcgaagcg gccggcagcg acgtcgacgg cctccagcac
      121 accccggctg aagtcgtcat acgtggtcag cgccttgccg cgctcgaccc ggttgccgac
      181 gacgactgag acgtcggtgt gggtcccgcc cacgtcgacc ccgacgaacg catccgcaga
      241 actaccgttg ctcgtgctgc tggtcatctc ttctcctcgt gcaggaatcg tcgtgccctc
      301 cgaaaccctg cccgcctccc agccgagcgg caactattcg aaccgacagt ctgtcagcgc
      361 agcgaggcgc tcgactccct catctgctcg cgcagaacga acttcttcgg cttgccaccg
      421 gcggttcgcg ggatctcctc gaccacgacc accgagcttg gcttcttgaa cccggcgagc
      481 cgctcgtagc agtagtcgat cagcgcctgc tcttcggtcg cggcgcccgg ttcgagctgg
      541 accacggcgc acaccacctc gccccatttg cggtcgggca ggccgatcac ggcgacgtcg
      601 tcgacctccg cgtgggtgcg caggacgtcc tcgatctcgg acgggaagac gttcatcccg
      661 ccgctgatga tcatgtcgtc cttgcggccg gagatgaaga ggaagccgtc ctcgtcgaag
      721 cggcccatgt cgccggtgtg caggaagccg ttgacgatca ggcggtcggt cagctcgggg
      781 tcggcccagt agccgggggt gaccgagggg gcgcggatca cgatctcgcc gtcgaccccc
      841 ggggcgacgt cttcgccgtc ctcgtcgacc acgcgcagct cgacgtgctg ggagggcagc
      901 ccggccgagc cggccttctc ggcggccttc gccggcggca ggaaggtgac cgagccgccg
      961 ccctcggtga gcccgtagac ctggatgaag tcggtcgccg ggaaggccgc ggcggcgcgt
     1021 tcggcggtgt cggccgagag cggctggccg ccgcacatga tcatcttcag cctgctcagg
     1081 tcgtactcgg cgaggttcgg caggcggagg atctcgtaga gcatcgtcgg cacccagagg
     1141 atgtgggtga ccccgtgctc ggcgacgtag gcgacggtct cctcggcgtc gaagttgctc
     1201 gacggcttga tgtggacggt gccgccctgg tgcaggatcg gccaggtgaa gtcgtggcgg
     1261 ccaccgatgt aatagaggtc gatgatcgcg tagctggcgt gctgcgggcc gagctggaag
     1321 tccatcgcct gctggatcga gttccagagc acggtcccgt cggaccagac cacgcccttc
     1381 ggctcgccgg tcgtgccgga ggtgaagatg atctcgtgca ggtcgtccat ccgcgccggg
     1441 cggcccgggt gctggccgct caccgaggcg cagagctcct cgtagccgag gtcgccggcg
     1501 ccctcgccga gcgcgatgat cgtgcgcagc tccggcacct cgccggtccg ccgcagttcg
     1561 gcgaccgtgt gcaggaagcg gtcctcgacg aagatcgtct gcgcgcccga gcggcgcagc
     1621 aggttggcca gctcgcccga gcgcaggcgc cagttgagcg gcaccatcac cccgcgcgcg
     1681 gccgagatcc cgacctcgat ctcgaggtac tcgagcgagt tggagctgag cagcgcgacc
     1741 cggtcgccag gttcgacgcc gatcccgatc agccccccgg cgacccgccg cgagcgatcg
     1801 cgcagttcgc cgtaggtgcg gctgcggtcg ccgaacacga ccgccttctt ctccaggtcg
     1861 aggcccccgc gctcgaacag gtacggcagc ccgagcgagt tccaccggtt cgccggggat
     1921 cggttcgggt catccatctg gccgtcgaaa ccgcgtatca agacctaact cccctccatc
     1981 gcaccgcacc gcggtcttct cgggcttaag tgggctgagg gtaacaccgc ccctgccgcc
     2041 cccaccacta gcgatatcgc cagtccccta cccccgattg gcgcattgct acacggcgag
     2101 cctgcatgac tccggcccga tctgcgccta ttctcgtagg cgtggctacg agctttgatc
     2161 cactaattgc cgcagatctc agcgaaccgc agcgcagtac cttgcagagc ttcgctaccc
     2221 gtctcaggga ggccctgggt gaagacctgc ttgctctttg gctttacgga tctcgggctc
     2281 ggggggaggg agttcatccg gagtccgacg ttgacctgat ggtgatcgcc aagggtggcc
     2341 gcgagcgcta tcagcgagtg gcctgggatc tcagtgaaga agctgcaata agcgagggag
     2401 agagcccgtt ctcctactca gtccacgttc acgaccgtga gtggctgagc cggcgccgcg
     2461 agatcgagtc cttcttcatc caagaggttg accgcgacaa gatcgtgatc actggaagcg
     2521 cattggaatg agtcctcgct ctgaggagtt catggagcag gcgcgcgagc gccttgccga
     2581 cgctcaagcg atcagcgacg ccgttcgtcc tgctctcgta gtcagcgcgg cctactactc
     2641 gatgctctat gcagctcgcg cagctctctc agagcgaggc gagttcgcaa agactcacag
     2701 cggaacctgg actctgttca gtcagcactt cgtcgcaacc ggagagttcg atcaggcatt
     2761 gagcgcgatg gcacgccgag cgaaggatgc ccgcgagcaa ggcgactacg aggcttctcc
     2821 gccaagcgcg gcggaagcga ccgagttcgt cgacggggcc gctgccttca tcgccgcaac
     2881 cgagcgcctg ctggacgact gatcgcgctc agactttgac ggtcgggacc gggtcttcgg
     2941 cgctgacggg gtcgctggcg gggatcggga tgctggcggt gaccttggtg ccgccggcgg
     3001 gcgcgcggcc gacgcgcagg cagccgccgt gctcgtcgag ggtgcggcgc agggaggcga
     3061 ggccgtggcc gggctcggag ttgctgcggg gggcgccgac gccgtcgtcc atcacggtca
     3121 gcagcaggcg ctcgcggtcg gtgaggctga gctggacgct gacccggcag gggccggcat
     3181 gcttggccgc gttgaccagg gcctcgcggg cggccttgat cagggcgtcg gcgggcggct
     3241 tgcgcaggcg gttggcgacc gcgatcgcgg cgtcgctgaa ctgggtgtgg atcgcgacgc
     3301 tgaactcctg ctcgaccccg acgatcgcgg cggcgaggcg ctggtcgaag tccggcgagg
     3361 ccgggttgga gaggcggtgg atcactttgc ggatctcggt atcgccgcgg accaggaggc
     3421 cgagcgcgcg ctcgaccccg gccgcgatcg tgctctcggc cggctcgcgc tcgaggatcg
     3481 cgtcgaggtt gagctgggcg gcgaagagga tctgggcgac gtcgtcgtgg aggtcgtcgg
     3541 cgatccgctg gcgctcctgc aggcgggcca gctcggcccg ctgctgctgg tagcgctcgg
     3601 tcgcgaagct cagcgcggcg aggttggcga gacggacgat cgcgcggcgc tcgtcgcggg
     3661 cgaagggctc gccgcgggtg cgcagggcgg cgagcgtgcc gacccgctgg cggccgaggt
     3721 ggacgggcgc gtagaagacc gcccagccgg ggtcgccggc cttctgcggg ccgaccacgt
     3781 aggcgcgcat gccgggcttg cgccgcacct cctccagcac cacgtcctcc agcgggctgg
     3841 cgccgtggcg ccagatcgcc ggctcgccct cggtcgggac catcgccagg aagctcgcct
     3901 cgaccgcccg cccgaccccc ttcagggccg ggttgaggaa gtcggtgacg ctctcgccgg
     3961 aggcgaccgc gaccgcgacc tgggagagga tctcctccag ccgctgctgg cggcgcaggc
     4021 ggaagagggt gcgggcgagc tcgaccgcgc cggcgatccg cgcggtcagc ccttcggccc
     4081 ggtcgaggtc ttcgacgccg aagtccgtgc ccgggttggc gatgtggagc acgccgatcg
     4141 ggcgcccggc cagggtcagc ggggtcgaga gcagcttctc gatcccgaag gcgttgacgt
     4201 attcctgcat gatccccggg tcgttgcggg cgtcgttcgt gtagtaggcg cggccggtgg
     4261 tgaagacgcg ggccgagttg ctgtgcgggt cgagcaccgg gatctggcag gagagcgcga
     4321 cctcttcgct ggcgccgaag gagccgccga ccatctgcag gacttcgcgg cgctcgtccc
     4381 agaccatgat cccggtcttc tccgcgtcga acagcggcgt caccgccttt tcgatcgccc
     4441 ggcagagctc ggcgaagttg tgcgcggcga ggaccagctc gtcgagcgcc gcgaggctct
     4501 ggccgcgcac ctccaggtgc aggcgcgagc ggtcctgatc catcgcgatc gccgcctggc
     4561 gggcgaaggt cgcgaccagg ccctcgcggg cgaacgagcg cggttcgcgc ttgaacgcgt
     4621 agaccgcgcc gagccgccgc ccctcgacca cgaccggcgc gaccagcacc gaggcgaagc
     4681 cgtggctgag cacgatgttg gcgccggcga tccgggtgcc ggcgaggaag gggatgccga
     4741 tccattcgcg gtcgccgaag tcgagcgccg agtcgaggtg cgtgagcccg cggcgcaggt
     4801 cgtcgaggtt gcggccggtg aggccgtcga cgccggcgac cgcgagcagc cgctcgccgg
     4861 tcggcgcccg ctcgacctcg gtgcgggcga tcaccaccgc gtcgctgccg agcgcgcgct
     4921 gcgcggcggc cgccagctcg cccaggtccg agcgctcttc ctccaccaac gccgggtcag
     4981 cggcggtcat agctcggcct gcgggacccc gtcctcgagc gccggcgcga taaagccggt
     5041 gcggatcgct ttcgcgatca attcggtctt cgttttcgcg gagaacttct ccttcagctt
     5101 ctgcacgtgg aagcgcacgg tcgactcgga gatgaacagc cggtcgccga tctccccgtt
     5161 ggtcagcccc tgggccgcca gttcgagcac cgcttcctgg cgcggggtcg ggatcgagtc
     5221 gtccatccgc gccgcgacca gcgagtgcag ctgcttgacg atctgcggcg ccgcggtgcc
     5281 gtcgaccgcg ccgtcgtcgg cgatcacccg gtcgatcgcc gcgcgcagct ccggcaggcc
     5341 ggcggccttg gtcacgtagg cggcggcgcc ggcgtcgagc gaggcccgca ccgtgccctc
     5401 gccgaggtag gtgctgagca cgatcaccgc ggtactgggg aagcgcgtgc ggatctcccg
     5461 ggtcaggtcc tcgccgcgca tgtccggcag gcgcagatcg accagggcga tgtcgggcag
     5521 cgtctgccgc gcccgttcca gcgcttcttt gccactggcg gcggtaccga cgatctcgaa
     5581 ccgctcgtcc gtggccagcg cggaggccaa accttccctg acgacctcgt ggtcgtcgac
     5641 gatcagcaaa cgcttctgca ctgcgtccct cttccctaga agccttctgg cgaggataca
     5701 cccgatcttg cgatattcaa cctgccgctc ggtcgggaaa ttgacctctc agagaacgtt
     5761 aaggcgctac cagcccatca ggctggccag gtcggcgcgc aggccaggga agacaaaacg
     5821 ggaccaggcc gcgcgaatat cgctcgattc gccctttgag tagaggtgca ggccggttcc
     5881 catgatcgag aacagcatca gcgcacccat ggctgcgaac acgttgacta ccgagcggtc
     5941 gacaggctgg ccggtcgccg cttcgtagcg ctcctccatc cacccgcgct ccgcgaccgc
     6001 cccgcagagg ggcgatccgg gggtcacgaa gcggccgccg tggtagtcga gcgagaggta
     6061 gccgaggtcg aagcgggggt cgccgatcga ggccagctcc cagtcgagca cgccgctgag
     6121 gcggcccttg tcgatcaggc agttgccgat ccggtagtcg ccgtggacga ggcgcggctg
     6181 caccgggtcg ggctcgttct cgagcagcca ggcgtaggcc tcctcgacca ccgggtcctc
     6241 gaccaagtgc acttcctcgt agatcgcgcg ccagcgggcg acggtctggc ggaagtcgcg
     6301 ggcgaccgcg gcgccggcca gcttcgccgg gtcgaccagg tggatcgccg ccatccggtc
     6361 gacgaagtcc tcggccaggg tgcggctgcc ctcccagttc tcggtcagct cgtcgcggtc
     6421 gcgctggcgc cagacgttga ccgccttgcc cggcagcttg tccatcagga agaaggggcc
     6481 gccgaaggcg gtctcatcgg cgggctggta gccgtggacg cgcggcgagg gcaccggctc
     6541 gtcctcgatc gcctcgtaga tcgcgaactc ctggccgagg tcggtgtcga gcaccgagtc
     6601 ggcgggtcgc acccggacga tcagctcgag cggcgcggcc tgatccgggt cggcgacgtc
     6661 gagcatgtag gtgtggcgcg accagccgcc ctcgagctgg gtcagcgagc tgacctcggc
     6721 gcgggtgccg gcggcgtcga ccgagcgcag cagctccagc gccgcggctg ccttggccgg
     6781 gtcgatcgcg gcctcggcgc tcacttcgac tccccccggc gctcgggtcg cgccgccagc
     6841 gcggccagct cgcgtgccgc catctcgcgc agcaccgcgt gcacccgttt acgcagctcg
     6901 gggtcgcggc cctcggcgcg cagctcggcc agcaggtcga ccagggccgc gccgatcgcg
     6961 tcggcgtcgc cggcctcggc gacccgctcg cgcgcgcttg ccgccgccgg gtcctcgctc
     7021 gcggccagcg ccgcgtcgag ctcgcccttc agccaggcca ggagccccgg cgtcgcccgc
     7081 acttccgccg ccagcaggcg gctgatgctg aaggcggaga gcagctggcc gcgcgcgtac
     7141 tcgtcggccg gaccgtcagc cgccgcggcg gcccgcatcg tcacccccat cccggcgatc
     7201 agctcgtcgg tgcgcagggc gttgaacatc aggagacctc cagggggttg atgtgggtta
     7261 gacgcgtgca cgggggcgac gcaggccgag ctgggcgccg cacgcgggcg aagcgggtag
     7321 tggttactac ccgcgagacc gtgggtggcg atccagtgac ggcatgcggc gtcatcc
//
LOCUS       DASRIG010000515         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3633150, whole genome shotgun sequence.
ACCESSION   DASRIG010000515 DASRIG010000000
VERSION     DASRIG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633150"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VFB52_13830"
     CDS             complement(<1..783)
                     /locus_tag="VFB52_13830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZO07464.1"
                     /translation="MTDNARKIRTLVVTATATVALSLPVALTSAQAATEPTPGDSIEQ
                     PKEGPLEQGATLAPPSSEDSEPVHNGGTNAEDGTPEPDPDEGEEDVETPSPDEADTEA
                     SPDPSGAFTVPAFPTSSCASTGVPPVLIPIYQRAAARYELGPQGPAVLAGINEIETGF
                     GQNLGPSYAGAVGWMQFMPETWAAYGVDADGDGDRDPNDPEDAIFSAAYYLSASGMPE
                     DTYGAIFAYNHADWYVADVLANAACFAQEVGGPGFGPGSLMPQ"
     gene            complement(799..1641)
                     /locus_tag="VFB52_13835"
     CDS             complement(799..1641)
                     /locus_tag="VFB52_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07465.1"
                     /translation="MIITGGDGLRALFELARRPFEWLAWVVRRGVLWPLQDRGGNLSA
                     PGRVLAAVGAALIVGVGVAALLSASSGSSDSEPAAAEVAVVTEKPEPAPAPKPVEAEP
                     GPTLEGAAPTFKPSKDEKQQAKAEGSTAPVTSTPADPNPATAKIGSKPGASVSSAPPS
                     SPQSAIGIDGPPAGPEAIAVAEDFAGAFVLYETGGEKEQVRKAFKATATPELTKSLLK
                     RPPRQPANVDVPKAKVVNVVAGPSHGSVYSVSVSLLRVGLTSELRLDMEKLKNDWRVT
                     NVLG"
     gene            complement(1841..2326)
                     /locus_tag="VFB52_13840"
     CDS             complement(1841..2326)
                     /locus_tag="VFB52_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07466.1"
                     /translation="MRSFSLALSLALVAVLAMTGSAGAVIVPQKSIAGVKLKMTRAQV
                     VAAKGKPDAERVVDNEILGRQRIMRYGRTQIGFNGEAADATVSGVRTTARGQRTRSGV
                     GRGSTQAAVEAGVPGVTCEDEFGVSHCFKGEFATGQRVTDFQLNDNGRVRSVFVGFVI
                     D"
     gene            complement(2416..>2762)
                     /locus_tag="VFB52_13845"
     CDS             complement(2416..>2762)
                     /locus_tag="VFB52_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HZO07467.1"
                     /translation="FLNRIDDTVIFHRLSKTDIGRIVELQVAQLVGRVRERGIEVELT
                     DDARTLLGNLGYDPVYGARPLKRVIQKQLVDKLALALLEGEFKEGDTVRVDTADGTLT
                     FERVGTTAPAAA"
BASE COUNT          378 a          909 c         1020 g          455 t
ORIGIN      
        1 ctgcggcatc aggctgccgg gcccgaagcc ggggccaccg acctcctggg cgaagcaggc
       61 ggcgttggcg agcacgtcgg cgacgtacca gtcggcgtgg ttgtaggcga agatcgcgcc
      121 gtaggtgtcc tccggcatgc ccgaggcgct gaggtagtag gcggcggaga agatcgcgtc
      181 ttcgggatcg ttcgggtcgc ggtcgccgtc gccgtcggca tcgaccccgt aggcggccca
      241 ggtttccggc atgaactgca tccagccgac cgcgccggcg taggaggggc cgaggttctg
      301 gccgaagccg gtctcgatct cgttgatccc ggcgagcacc gccgggccct gcgggccgag
      361 ctcgtagcgg gcggcggcgc gctggtagat cgggatcagg accgggggca ccccggtcga
      421 ggcgcagctg gaggtcggga aggcggggac ggtgaaggcg cccgagggat ccggcgacgc
      481 ctcggtgtcg gcttcgtccg ggctcggcgt ctcgacgtct tcttcgcctt cgtcggggtc
      541 gggttcgggc gtcccgtctt cggcgttggt gccgccgtta tgaaccggct cggagtcttc
      601 ggacgagggc ggcgccaggg tcgcgccctg ctcgagcggt ccttctttcg gctgctcgat
      661 cgagtcgccc ggggttggct cggtggcagc ctgggcgctg gtcagcgcga ccggcaacga
      721 gagcgccaca gtggcggtcg cggtgacgac gagggtgcgg atcttgcgag cgttgtcggt
      781 cacggtcggt tccagccctc agccgaggac gttggtgacg cgccagtcgt ttttcagctt
      841 ttccatgtcg agccgcagct cgctggtcag gccgacccgc agcagcgaga cgctgaccga
      901 gtagacgctg ccgtgcgagg ggccggcgac gacgttgacg accttggctt tggggacgtc
      961 gacgttggcg ggctggcgcg gcgggcgctt cagcagcgac ttcgtcagtt cgggggtggc
     1021 ggtggccttg aaggctttgc gcacctgctc cttctcgccc ccggtctcat agaggacgaa
     1081 ggcgccggcg aagtcttcgg cgaccgcgat cgcctcgggc ccggcgggcg ggccgtcgat
     1141 cccgatcgcc gactgcggcg aggagggcgg tgcggaggag acggaggcgc cgggtttgga
     1201 gccgatcttc gccgtcgccg ggttggggtc ggcgggcgtg ctggtgaccg gcgcggtcga
     1261 gccctcggcc ttcgcctgct gcttctcgtc cttgctcggc ttgaaggtcg gcgcggcgcc
     1321 ctcgagggtc ggtccgggct ctgcttcgac cggcttcggc gcgggggcgg gctcaggttt
     1381 ctcggttacc accgcgacct cggcagcggc cggttcggaa tcgctgctgc cggagctggc
     1441 gctgagcagg gcggcgacgc cgacgccgac gatcagcgcc gcgccgactg ccgccagcac
     1501 acgaccggga gcgctgagat tgccgccgcg atcctgcagc ggccagagca ccccgcggcg
     1561 cactacccag gcgagccact cgaacggacg gcgggcgagc tcgaacaacg cccgcagccc
     1621 atcgccgccg gtgatgatca cccactcgac ccaccaagcc gagatccgct tggcagcgcg
     1681 agacagccgg gtttgccggc gctcagcgcg gttgatcgga gtttcttgac tatccatggc
     1741 cgggtgtcag agtgcaacgg aagcttaagg cgcaaattaa gaacgcaccc gtcacccgta
     1801 gtttcggcag aaacccgctc ttatttgagc gctgaaagcg ctaatcgatc acgaagccga
     1861 cgaaaaccga gcgcacccgg ccgttgtcgt tgagctggaa gtcggtgacc cgctggccgg
     1921 tggcgaattc gcccttgaag cagtgggaga cgccgaactc gtcctcgcag gtgacgccgg
     1981 gcacgcctgc ttcgaccgcg gcctgggtcg aaccccgccc gaccccgctg cgcgtgcgct
     2041 gaccgcgcgc ggtcgtgcgc accccgctca ccgttgcgtc ggccgcctcg ccgttgaagc
     2101 cgatctgcgt gcgcccatac cgcatgatcc gctgccgccc gaggatctcg ttgtcgacca
     2161 cgcgctccgc gtcgggtttc ccctttgccg cgaccacctg cgcacgcgtc atcttcagct
     2221 tcacgcccgc gatgctcttc tgcggcacga tcaccgcccc ggccgagccg gtcatcgcca
     2281 agaccgcgac cagcgcgagg gagagagcga gcgagaacga gcgcacgccg cgagtctagg
     2341 cccgcccaag cgaactcgct tgtcgcgagt ccgtcgttac ccgtcctaga gacgggtagg
     2401 agcagactcg tgggtctagg cggcggcggg ggcggtggtg ccgacgcgtt cgaaggtgag
     2461 ggtgccgtcg gcggtgtcga cgcggacggt gtcgccttct ttgaactcgc cctcgagcag
     2521 ggccagggcg agtttgtcga ccagctgctt ctggatcacc cgcttcagcg ggcgggcgcc
     2581 gtagacgggg tcgtagccga ggttgccgag cagggtgcgg gcgtcgtcgg tcagctcgac
     2641 ctcgatcccg cgctcgcgca cgcggccgac cagctgggcg acctggagct cgacgatgcg
     2701 gccgatgtcg gtcttcgaga ggcggtggaa gatcaccgtg tcgtcgatcc ggttgaggaa
     2761 ct
//
LOCUS       DASRIG010000516         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_247415, whole genome shotgun sequence.
ACCESSION   DASRIG010000516 DASRIG010000000
VERSION     DASRIG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247415"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFB52_13850"
     CDS             <1..488
                     /locus_tag="VFB52_13850"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZO07468.1"
                     /translation="GGPTPTMLPATTSPLVDQGSGFGLLADQRGVVRPIDFPSIPNAT
                     GGNGSDIGAVELQPSNAFGFGKLQKNKKKGTAKLTVNLPTPNVGTLTLTGKGLKKRTV
                     ALNGSKSRLTLLVATKGKAKKALRSKGKRKVAIKVTYTPTANAPLTKVRKTKLVKKLK
                     K"
     gene            507..1997
                     /locus_tag="VFB52_13855"
     CDS             507..1997
                     /locus_tag="VFB52_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZO07469.1"
                     /translation="MEAKAGEGIAFESSAGRWMLAVAVAGSGMAFLDGTVVNVALPMI
                     GEDFDASTSALQWIVNGYLLTLASLILLGGSLGDRYGRRRVFSIGVGLFTVASALCAV
                     APNEEMLIAARLLQGIGGALLTPGSLALIEASFRPTDRARAIGAWSGLGGVAAAIGPL
                     VGGYLIGAISWRAIFLINIPLGAFVIWAAARHVPESRDPNASKHLDLIGSVLTALGLA
                     GTTYALIEAPERGASAAILVAGIGGLAALVAFLVHERRTANPMLPLGIFSSRQFSAAN
                     GVTFVVYAALGGVFFFLVSFLQISMGYTPIEAGSASLPVTGLMLVFSARAGALAQRIG
                     PRIPLTVGPLVIGLGLAWLSMVEPGDSYLAAILPPIVVFGIGLTLVVAPVTATVLAAA
                     DSNHSGIASGVNNAVARVAGLLAVAVLPVLAGLTGDKFYEAEAMTDGFQIGMMACAAL
                     AVAGGVLAWFTIDSEVLHEEPGQPEPEPEYSCGVNGPPLLPGREMG"
     gene            1994..>3027
                     /locus_tag="VFB52_13860"
     CDS             1994..>3027
                     /locus_tag="VFB52_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZO07470.1"
                     /translation="MKIRAAVLEEFGAPLAVQELELTEPRAGEVLVRLVACGVCHTDL
                     YTASGADPSGYAPAVLGHEGAGVVEAVGEGVSSVSPGDHVVTLFSPQCRECVHCVDDR
                     TNLCMAIRAEQNLGHLPDGTTRLQRDGEEVRHFMGCSTFAEYTVMPEIALAKVNPEAP
                     LEHACLFACGLSTGLGAAINTAKVEPGTTCVVFGAGMVGLGAVAGCRLQGAERIVCVD
                     LSEERLELARGQGATELMVAGPDTIGEIIESTDGFGADYTFEATGNVKVMAQAVEAAR
                     MAWGLCTVAGVAGKGETLDVIPRWLITGRRVAGSSFGGVKGRDQVPEFVERVMAGEID
                     VEPFVSHKITL"
BASE COUNT          432 a         1111 c         1033 g          451 t
ORIGIN      
        1 acggcggccc gaccccgacg atgctgccgg ccacgaccag cccgctggtc gaccagggct
       61 cgggcttcgg cctcctcgcc gaccagcgcg gcgtcgtccg cccgatcgac ttcccctcga
      121 tcccgaacgc gaccggcggc aacggctccg acatcggcgc ggtcgagctg cagccctcga
      181 acgcgttcgg cttcgggaaa ctgcagaaga acaagaaaaa agggacggcg aagctgaccg
      241 tcaatctgcc gacccccaac gtcggcaccc tcactctgac cggcaaaggg ctgaagaaac
      301 ggacggtcgc gctcaacggc tcgaaatcgc gcctcacgct gctcgtcgcg accaagggca
      361 aagcgaagaa agcgctgcgc agcaaaggca agcgcaaagt cgcgatcaaa gtcacctaca
      421 cgccgaccgc gaacgcgccg ctaaccaaag tccgcaagac caagctggtc aagaagctga
      481 agaaatagcg cagctaccat cgcccgatgg aggcgaaagc aggcgagggc atcgccttcg
      541 agagcagcgc cggccgctgg atgctggccg tcgccgtcgc cggctccggg atggcgttcc
      601 tcgacggcac cgtggtcaac gtcgcgctgc cgatgatcgg cgaggacttc gacgccagca
      661 ccagcgccct gcagtggatc gtcaacggct acctgctgac cctcgcctcg ctgatcctgc
      721 tcggcggctc acttggcgac cgttacgggc gccggcgggt cttctcgatc ggggtcggcc
      781 tcttcaccgt cgcttccgcg ctctgcgcgg tggcgccgaa cgaggagatg ctgatcgccg
      841 cgcggctgct ccaggggatc ggcggcgccc tgctcacccc cggcagcctg gcgctgatcg
      901 aggccagctt ccgccccacc gaccgcgccc gcgcgatcgg cgcctggtcg gggctcggcg
      961 gcgtcgccgc ggcgatcggg ccgctggtcg gcggctacct gatcggcgcg atctcatggc
     1021 gggcgatctt tctgatcaac atcccgctcg gcgccttcgt gatctgggcc gccgccaggc
     1081 acgtgccgga gagtcgcgac ccgaacgcca gcaaacacct cgacctgatc ggctcggtgc
     1141 tgaccgcgct cggcctcgcc ggcaccacct acgcgctgat cgaagcgcct gagcggggcg
     1201 cctcggcggc gatcctcgtc gccgggatcg gcggcctcgc ggcgctggtc gccttcctcg
     1261 tccacgagcg ccgcaccgcc aacccgatgc tgccgctggg gatcttctcc tcgcgccagt
     1321 tcagcgccgc caacggcgtc accttcgtcg tctacgcggc gctcggcggc gtcttcttct
     1381 tcctcgtctc cttcctgcag atctcgatgg gctacacgcc gatcgaggcc ggctccgcct
     1441 cgcttcccgt gaccgggctg atgctggtct tctccgcccg cgccggggcg ctggcgcagc
     1501 ggatcgggcc gcggatcccg ctgacggtcg ggccgctggt gatcggcctc gggctcgcct
     1561 ggctgagcat ggtcgaaccc ggcgactcct acctcgccgc gatcctgccg ccgatcgtcg
     1621 tcttcggcat cggcctcacc ctggtcgtcg ccccggtcac cgcgaccgtg ctcgccgccg
     1681 ccgactccaa ccactcgggg atcgcctccg gggtcaacaa cgcggtcgcc cgcgtcgccg
     1741 gcctgctcgc ggtcgcggtg ctgccggtgc tcgccgggct gaccggcgac aagttctacg
     1801 aagccgaggc gatgaccgac ggcttccaga tcgggatgat ggcctgcgcc gcgctggccg
     1861 tggcgggcgg cgtgctcgcc tggttcacga tcgactccga agtgctgcac gaggagcccg
     1921 gccagcccga gccggagccc gagtacagtt gcggcgtcaa cggaccgcca ctgcttcctg
     1981 gaagggagat gggatgaaga ttcgcgccgc tgtgctggag gagttcggag cgcccttggc
     2041 ggtccaggag ctggagctga ccgagccacg ggccggcgag gtgctggtgc ggctggtcgc
     2101 ctgcggcgtc tgccacaccg acctctacac cgcctccggc gccgacccct ccggctacgc
     2161 gccggcggtg ctcgggcacg agggcgcggg cgtggtcgag gcggtcggcg agggcgtctc
     2221 ctcggtctcc cccggcgacc acgtcgtcac cctgttctcg ccgcagtgcc gcgagtgcgt
     2281 gcactgcgtc gacgaccgca ccaacctctg catggcgatc cgtgccgagc agaacctcgg
     2341 ccacctgccc gacgggacca cgcggctgca gcgcgacggc gaggaggtgc gccacttcat
     2401 gggctgctcg accttcgccg agtacacggt gatgccggag atcgcgctcg ccaaggtcaa
     2461 cccggaggcg ccgctcgagc acgcctgcct gttcgcctgc gggctctcga ccgggctcgg
     2521 cgccgcgatc aacaccgcga aagtcgaacc cggcaccacc tgcgtcgtct tcggcgccgg
     2581 catggtcggg ctcggcgccg tcgccggctg ccgcctgcag ggggccgagc ggatcgtctg
     2641 cgtcgacctc tccgaggagc ggctggagct ggcccgcggc cagggcgcga ccgagctgat
     2701 ggtcgccggc cccgacacga tcggcgaaat catcgagtcg accgacggct tcggcgccga
     2761 ctacaccttc gaggcgaccg gcaacgtcaa ggtgatggcg caggcggtcg aggcggcgcg
     2821 gatggcctgg ggcctctgca ccgtcgccgg cgtcgccggc aagggcgaga cgctcgacgt
     2881 gatcccgcgc tggctgatca ccggccgccg cgttgccggc tcttcgttcg gtggggtcaa
     2941 ggggcgcgac caggtgccgg agttcgtcga gcgggtgatg gccggcgaga tcgacgtcga
     3001 gcccttcgtc tcgcacaaga tcacgct
//
LOCUS       DASRIG010000517         6450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1799332, whole genome shotgun sequence.
ACCESSION   DASRIG010000517 DASRIG010000000
VERSION     DASRIG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799332"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFB52_13865"
     CDS             complement(<1..683)
                     /locus_tag="VFB52_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07471.1"
                     /translation="MAVWGIRISAIALLAALSLTLAVLLPGGSSERADAAAAPKVPTQ
                     ARYLLRLTDLPPGYRLAVSAEFLSVGGVRCAPIEPADPRPQLKRFVRRAPRGCMALYG
                     DTFRSPGPGPEPALVGTGVMRLKSVAAAEEGLDLAPQLISHAINDELPAEVPAPAQVG
                     ERTRIFHWSYPGLFGEELATFLVWRWGGAVAAIFTAGGEAAANDAVALGLAQRQQRHL
                     EEPEPFSPA"
     gene            762..1865
                     /locus_tag="VFB52_13870"
     CDS             762..1865
                     /locus_tag="VFB52_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZO07472.1"
                     /translation="MAAGTISFARGAPSADILPHEAVRDAAARALAEDWETALSYGVG
                     IGHPGLCKWIAERHGDIDPDQVMVANGSLEAGAMLFEHLLAKGDRVVVEQPTYDRTLL
                     LLQKLGVELVPVPLESDGLDVGALEAALAEGPIRLAHIIPNSHNPAGCTLSLEKRQRL
                     VELAAEHGFWIFEDDPYRELPFEGEPLTTMLTLDQASRVIHASSFSKTVSPGVRVGYL
                     AGPADEIKKIAKRANETYISPNMLAESIVLELCRSGELDRNIEFVKGELKARCDALVA
                     ALRERIPEAEFVVPEGGYFLWLDLAEGTDTVALLNEAKADGVSFVAGPDFMIEGGQNS
                     LRLSFAPVPADQADEGVARIAQALERLRSASPA"
     gene            1967..2671
                     /locus_tag="VFB52_13875"
     CDS             1967..2671
                     /locus_tag="VFB52_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO07473.1"
                     /translation="MAGRIGSTNERDGNAIARAAKDPVSRRKFLALTGGSGAAAAFLA
                     ACGSDDSSSTMGTTTGANEMGGGMADSETAQFGKGDLGIVNYALTLEYLEAAFYADVV
                     KSGLFKGAELKAIEKFGQEEAEHVEALTQAAKALGGKPAAEPKAEFPLEDAKSVVELA
                     GTVENLGAAAYLGQAANIKSPEVLASALAIHSVEGRHAATLNGMLGVSITPDGAFAKP
                     ADAATVLKSVEPFIVG"
     gene            2700..3341
                     /locus_tag="VFB52_13880"
     CDS             2700..3341
                     /locus_tag="VFB52_13880"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZO07474.1"
                     /translation="MSQEQLAHPELVAGITVDDDSQDLSRSEVILKGALAAGAVYGTF
                     MVGPYVRKALAMSGSSDVDILNFALTLEHLETKFYEEAKSRAGAKGELKSLIDLIAKD
                     EAQHVEALTATVKQLGGKPVAEPKFDFEYNDTAGFLKLAQTFEDTGVSAYNGAGPAIK
                     SKDVLAAAGSIVQVEARHAAAIRIQNGEEPSPDAFDPPLEEAEVLKAVEPFIA"
     gene            3404..3985
                     /locus_tag="VFB52_13885"
     CDS             3404..3985
                     /locus_tag="VFB52_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO07475.1"
                     /translation="MTIRREKAERLADEELMPLIGKKDPEAFEVFYDRHGGVAYSLAY
                     RIVGERAAAEDVTQEAFISLWRSGARFDSTRGSVRSWMLSIVRNRAIDQLRSKAGKAP
                     KLTFDDDSVLEQRPADELTEEEALRRETASEVRGALGSLPGDQSKVIELAYYGGFSHS
                     EIAQMLGVPLGTVKGRMRLGLEKIRGELAEGLA"
     gene            3982..4710
                     /locus_tag="VFB52_13890"
     CDS             3982..4710
                     /locus_tag="VFB52_13890"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZO07476.1"
                     /translation="MTDDSNMTWFDQLAGYVLGALEPEEAAEVEAHLADCERCQAHLR
                     WLEPAVAVLPESVERQEPPKRVRENVMAEVRADAKRAQAESAESEGFRLRDGWLGKLR
                     SSAYGWKPAVALATLALLVVAFAGYEIGSNGGESGGSEAPIARSGQAPGVVAEMIPEG
                     EGGKLKLTNVEQVPDDRVLEAWVQREGEVEAVPDLFVPNHEGRASTTIEDMDGVEIVM
                     VTHEPEGGSATPTSDPIVTMPIPD"
     gene            complement(4707..5420)
                     /locus_tag="VFB52_13895"
     CDS             complement(4707..5420)
                     /locus_tag="VFB52_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZO07477.1"
                     /translation="MSSEQATVAEQEYLEIMFWIEEAGLPMTGANIARAMQLSPPTVH
                     EMVGRLIGDGYVERQANKSLNFTESGREHASFIVRRHRMIERFLTDVLGIPWDEVHEE
                     AERIEHAMSPVLEEKMRLAIGDATTCPHGHPIVEGIREQGSLLADVAVDADVTVLRFE
                     NEAEELLHYLKDAGLNPGLTGKIESSDDEKVVIASSDGKHEVSRSVAETVSVRADPAP
                     PPRAAIPEQLVLSSDRYGR"
     gene            complement(5494..6306)
                     /locus_tag="VFB52_13900"
     CDS             complement(5494..6306)
                     /locus_tag="VFB52_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZO07478.1"
                     /translation="MPLRCVYTDLDGTLLGPGGGLFRDAEGGFSMAQARAIEACHRAG
                     VEIVVMSGRREPQVQELARLMGQRSYIYEAGCAFAIDRETTLLTGDLQPSEDGTIYEQ
                     MEARGTPRALFDHFEGRLEYHDPWHHGRVLSHLFRGKVDVEEANALLAERGEGDLRLL
                     DNGAIGTPMPTVEGRTHAYHLVPKMVSKANAAAAHARAQGYAPSECIAVGDSVEDLEV
                     AAAVGRFFVVANGPERDPGLRASLTTWDNVTVTEGAMGDGFYEAVVTTLMER"
BASE COUNT         1041 a         2188 c         2284 g          937 t
ORIGIN      
        1 tcagccggcg agaagggctc cggttcttcg aggtggcgct gctggcgctg ggcgagcccg
       61 agcgcgacgg cgtcgttggc ggcggcttca ccgccggcgg tgaagatcgc ggcgactgca
      121 ccaccccagc gccagaccag gaaggtcgcc agttcctcgc cgaacagccc agggtagctc
      181 cagtggaaga tgcgcgtgcg ttcgcccacc tgggccggtg cggggacctc ggcgggcagc
      241 tcgtcgttga tcgcgtggct gatcagctgc ggggccaggt cgaggccttc ttcggccgcg
      301 gcgacgctct tcagccgcat cacgccggtg ccgacgagcg ccggctccgg tcccggtccc
      361 gggctgcgga aggtgtctcc gtagagcgcc atgcagccgc gcggcgcacg gcgcacgaac
      421 cgcttcaact gcggccgcgg gtccgccggt tcgatcggag cgcagcggac cccgccgacg
      481 gacaagaact ctgccgagac ggcgagccga tagcccggcg gcaggtcggt gagccgcaac
      541 aggtagcgag cctgcgtggg gacctttggg gcggcggccg cgtcggcgcg ctcggacgag
      601 ccgccgggca ggagcacggc gagcgttagc gagagcgctg cgagcaaagc gatggcgctg
      661 atcctgatcc cccaaaccgc cattcggcgc gagtataggc gcgttccgcg gcggtcgagg
      721 aaggggcggt cgagaccagg gcgttgctag ggtcggtggc gatggctgca ggcacgatct
      781 cattcgcccg cggcgctccg agcgccgaca tcctcccgca cgaggcggtg cgcgacgcgg
      841 ccgcgcgggc gctcgccgag gactgggaaa ccgcgctctc ctacggggtc ggaatcggcc
      901 atcccggcct ctgcaagtgg atcgccgagc gccacgggga catcgacccc gatcaggtga
      961 tggtcgccaa cggttcccta gaggcgggcg cgatgctgtt cgagcacctg ctcgccaagg
     1021 gcgaccgggt ggtcgtcgag cagccgacct acgaccgcac gttgctgctc ctgcagaagc
     1081 tcggggtcga gctggtgccg gtcccgctgg agtccgacgg tctcgacgtc ggcgcgctgg
     1141 aagcggcgct cgccgagggc ccgatccggc tcgcccacat catccccaac tcgcacaacc
     1201 ccgccggctg cacgctctcg ctggagaagc gccagcggct ggtcgagctg gccgccgagc
     1261 acggcttctg gatcttcgag gacgacccgt atcgcgagct gcccttcgag ggcgagccgc
     1321 tgaccacgat gctgacgctc gaccaggcga gccgcgtgat ccacgcttcc tcgttctcga
     1381 agacggtcag ccccggcgtc cgcgtcggct atctcgccgg ccccgccgac gagatcaaga
     1441 agatcgccaa acgcgccaac gagacctaca tctcgccgaa catgctggcc gagtcgatcg
     1501 tgctcgaact ctgccgctcg ggtgagctcg accgcaacat cgagttcgtc aaaggcgagc
     1561 tgaaggcgcg ctgcgacgcg ctcgtcgccg ctctgcgcga gcggatcccg gaggccgagt
     1621 tcgtcgtccc cgagggcggc tacttcctct ggctcgacct cgccgagggc accgacacgg
     1681 tcgccctgct gaacgaggcg aaagccgacg gcgtctcctt cgtcgccggc cccgacttca
     1741 tgatcgaggg cgggcagaac agcctgcgcc tctccttcgc cccggtgccg gccgaccagg
     1801 ccgacgaggg tgtggcccga atcgcgcagg cgctcgagcg cctgcggtcc gcctccccgg
     1861 cctagttcct acgcccccgc gttccgttcg gatcaatact gatccgcctc ttcgccgccc
     1921 gccgtaggga accggcgaaa gctcaaattc gcgaggaggc ggttgcatgg ctggcaggat
     1981 tggaagtacg aacgagcggg acgggaacgc gatagcgcgg gccgccaagg acccggtctc
     2041 gcgacgcaag ttcctcgcgc tgaccggcgg ctccggcgcc gccgcggcct tcctcgcggc
     2101 ctgtggcagc gacgacagct ccagcacgat ggggacgacg accggcgcga acgagatggg
     2161 cggcggcatg gccgacagcg aaaccgcgca gttcggcaag ggcgacctcg ggatcgtcaa
     2221 ctacgcgctc accctcgagt acctcgaggc cgcgttctac gccgacgtcg tcaagagcgg
     2281 gctgttcaaa ggagccgagc tgaaggcgat cgaaaagttc ggccaggagg aggccgaaca
     2341 cgtcgaagcc ctgacccagg ccgcgaaggc gctgggcggc aagccggccg ccgaaccgaa
     2401 agccgaattc ccgctcgaag acgcgaaatc ggtggtcgaa cttgccggga cggtggagaa
     2461 cctcggcgcc gccgcctacc tcggccaggc cgccaacatc aagagccccg aagtgctcgc
     2521 ctcggcgctc gcgatccaca gcgtcgaggg ccgccacgcg gcgaccctga acgggatgct
     2581 cggcgtctcg atcacccccg acggggcgtt cgccaagccc gccgacgccg cgaccgtcct
     2641 caaatccgtc gaacccttca tcgtcggctg agccggcgct gagcgaacag gagaacgcaa
     2701 tgagccaaga gcagcttgca catccagagc tcgtcgccgg gatcaccgtc gacgacgaca
     2761 gccaggacct ctcgcgcagc gaggtgatcc tcaagggcgc gctcgccgcc ggtgccgtct
     2821 acggcacctt catggtcggc ccgtacgtgc gcaaggcgct ggcgatgagc ggcagcagcg
     2881 acgtcgacat cctcaacttc gcgctcacgc tcgagcacct ggagacgaag ttctacgaag
     2941 aagcgaagtc gcgggccggg gcgaagggcg aactgaagag cctgatcgac ctgatcgcca
     3001 aagacgaggc ccagcacgtc gaagcgctga ccgcgacggt caaacagctc ggcggcaaac
     3061 cggtcgccga accgaaattc gacttcgaat acaacgacac cgccggcttc ctcaaactgg
     3121 cgcagacctt cgaggacacc ggggtcagcg cctacaacgg ggccgggccg gcgatcaagt
     3181 cgaaggacgt gctcgccgcg gccggctcga tcgtccaggt cgaggcgcgc cacgccgccg
     3241 cgatccggat ccagaacggc gaggaaccgt cgcccgacgc gttcgacccg ccgctcgaag
     3301 aagccgaagt cctgaaagcg gtcgaaccgt tcatcgcgtg atcctttgaa ggaacccgta
     3361 cgtaggaacg actgaaccca tgcgtggcga gaatcgatag acgatgacga tccggaggga
     3421 gaaggccgag cggctggccg acgaggagct gatgccgctg atcggcaaga aggacccgga
     3481 ggcgttcgag gtcttctacg atcgccacgg cggcgtcgcc tactcgctcg cctaccggat
     3541 cgtcggcgag cgagcggccg ccgaggacgt cacccaggag gccttcatct cgctctggcg
     3601 cagtggtgct cgcttcgact cgacccgcgg cagcgtgcgc tcgtggatgc tcagcatcgt
     3661 ccgcaaccgg gcgatcgacc agctgcgcag caaggccggc aaagcgccga agctgacctt
     3721 cgacgacgac tcggtgctcg agcagcgccc ggccgatgag ctgaccgagg aggaggcgct
     3781 gcggcgcgag accgcctcag aggtgcgcgg cgcgctcggc tcgctcccgg gcgaccagtc
     3841 gaaggtgatc gagcttgcct actacggcgg cttcagtcac tcggagatcg cccagatgct
     3901 gggcgtgccg ctgggcacgg tgaagggccg gatgcggttg gggctggaaa agattcgcgg
     3961 cgagctggcg gaggggttgg cgtgaccgac gattccaaca tgacctggtt cgaccagctg
     4021 gccggctacg tgctgggcgc cctggagccc gaggaggcgg ctgaggtcga ggcccacctg
     4081 gccgactgcg agcgctgcca ggcccacctg cgctggctgg agccggcggt ggcggtgctg
     4141 ccggagagcg tcgagcgcca ggagccgccg aaacgggtgc gcgagaacgt gatggccgag
     4201 gtgcgcgccg acgcgaagcg ggcgcaggcc gagtccgcgg agtccgaagg cttccgcttg
     4261 cgcgacggct ggctcggcaa gctgcgctcc agcgcttacg gctggaagcc ggcggtggcg
     4321 ctggcgaccc tggcgcttct cgtcgtcgcc tttgccggct acgagatcgg cagcaacggt
     4381 ggcgagagcg gtggcagcga agctccgatc gcccgctccg gccaggcgcc gggcgtcgtc
     4441 gccgaaatga ttccggaggg cgaaggcggc aagctgaagc tgaccaacgt cgagcaggtc
     4501 ccggacgatc gcgtgctcga ggcctgggtc cagcgcgaag gcgaggtcga agcggtcccg
     4561 gacctcttcg ttcccaacca cgaaggccgc gcctcgacca cgatcgaaga catggatggg
     4621 gtcgaaatcg tgatggtcac gcacgagccg gaaggcggca gcgcgacgcc gaccagcgac
     4681 ccgatcgtga cgatgccgat tcccgactag cggccgtaac ggtcgctgct gagcacgagc
     4741 tgctcgggga tcgcggcgcg cgggggcggg gccgggtcgg cgcgcacgga gacggtctcg
     4801 gcgacgctgc gggagacctc gtgcttgccg tcgctggagg cgatcacgac cttctcgtcg
     4861 tcggagctct cgatcttgcc cgtcaggccc gggttgaggc cggcgtcctt gaggtagtgg
     4921 agcagctcct cggcctcgtt ctcgaagcgc agcacggtga cgtcggcgtc gaccgcgacg
     4981 tcggccagca gcgagccctg ctcgcggatg ccctcgacga tcgggtggcc gtgcgggcag
     5041 gtggtggcgt cgccgatcgc gagccgcatt ttctcttcca gcaccggcga catcgcgtgc
     5101 tcgatccgct cggcctcctc gtgcacctcg tcccagggga tccccagcac gtcggtgagg
     5161 aagcgctcga tcatccggtg gcggcgcacg atgaagctgg cgtgctcgcg gccggactcg
     5221 gtgaagttca gcgatttgtt cgcctggcgc tcgacgtagc catcgccgat caggcggccg
     5281 accatctcgt gcacggtcgg gggcgagagc tgcatcgcgc gggcgatgtt ggccccggtc
     5341 atcggcaacc cggcctcctc gatccagaac atgatctcca ggtactcctg ctcggcgacg
     5401 gttgcctgct ccgacgacac gctgctcccc tctcgattgg gcttacggga ccgatagtag
     5461 tcggccggag ttctcgccgc gaagcctgcg gtactagcgc tccatcaggg tggtgacgac
     5521 cgcctcgtag aagccgtcgc ccatcgcccc ctcggtgacc gtgacgttgt cccaggtggt
     5581 gagtgacgcg cgcaggccgg ggtcgcgctc ggggccgttg gcgacgacga agaagcggcc
     5641 gacggcggcc gcgacctcga ggtcctcgac cgagtcgccg accgcgatgc actcgctggg
     5701 cgcgtatccc tgggcgcgcg cgtgggcggc ggcggcgttg gccttgctga ccatcttcgg
     5761 gaccaggtgg taggcgtggg tgcggccctc gacggttggc atcggggtcc cgatcgcgcc
     5821 gttgtcgagc aggcgcaggt ccccttcgcc gcgctcggcg agcagtgcgt tggcctcctc
     5881 gacgtcgact ttgccgcgga agaggtggga gaggacgcgg ccgtggtgcc acgggtcgtg
     5941 gtactcgagc cggccctcga agtggtcgaa gagcgcccgc ggcgtgcccc gcgcctccat
     6001 ctgctcgtag atcgtgccgt cctcgctggg ctgcaggtcg ccggtcagca gggtggtctc
     6061 gcggtcgatc gcgaaggcgc agccggcctc gtagatgtag gagcgctggc ccatcagccg
     6121 cgccagctcc tgcacctgcg gctcgcggcg tcccgacatc acgacgatct cgaccccggc
     6181 ccggtggcag gcctcgatcg cccgcgcctg cgccatcgag aagccgccct cggcgtcgcg
     6241 gaagaggcca ccgcccggcc cgagcagggt cccgtcgagg tcggtgtaga cgcagcgcag
     6301 gggcatcgac cgtcaaggct attgccgctt cgcgccagat gcgcgaccac ccacttttca
     6361 ctacagattg gtttcccatt tcaatcagat cagtagtgtg cgccgcaatg cgttggggga
     6421 ttgcagtact tctcgtcatg gtggccgcgg
//
LOCUS       DASRIG010000518         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_767840, whole genome shotgun sequence.
ACCESSION   DASRIG010000518 DASRIG010000000
VERSION     DASRIG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767840"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFB52_13905"
     CDS             <1..218
                     /locus_tag="VFB52_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07479.1"
                     /translation="PFFVAEQFTGLSGKYVPVEETVRSFKEIIEGKHDEIPERAFLLK
                     GTIDDVVNSVRGSDAKEEKQEDEGGEE"
     gene            395..805
                     /gene="atpC"
                     /locus_tag="VFB52_13910"
     CDS             395..805
                     /gene="atpC"
                     /locus_tag="VFB52_13910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZO07480.1"
                     /translation="MAAEHMIDVEILTPEGEVFEGEARQVSTRTEVGEIGILANHTPL
                     LAALKPTELRLKVSDSETKRYAQAHGWLQVFGNRARLLVEEAIAPEDLDAGVLKEQLA
                     DAEQRLSASDEDSAEHARAKKDLDRAEAFLAITG"
     gene            847..1938
                     /locus_tag="VFB52_13915"
     CDS             847..1938
                     /locus_tag="VFB52_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZO07481.1"
                     /translation="MTDELISLAQRLISFETTDPDGISEAAGFVQGWLGARGIEAKGS
                     EVRGLPVVMAEVGPADAPTVVLHGHIDVVPGHDDQFLPRVEGDKLIGRGAYDMKGAVA
                     AMMLVAASMRDQDRVRVRLGIVGDEESEEENERGSDHLVDSGFLGDFAITGEPTDLHI
                     GIEAKGVLALRLEVGGRAAHGATPWLGDNAVLNAIDVFRSIESLPFARHSSELFDRPS
                     INLGRIWGGDALNKVPDRCIIDVDIRYLPEQDPAGILEEVRALPGCEPTPLLTRPPAT
                     VERNSPFVLGLREAARAHHDGEPMSVGRDGASDAVSFLRVGVPAVEFGPVGAGHHGPE
                     EWVSVSSLATYRQALESFLIAIPDRLGDE"
     gene            1931..3625
                     /locus_tag="VFB52_13920"
     CDS             1931..3625
                     /locus_tag="VFB52_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZO07482.1"
                     /translation="MSEPLGPGWAPKKPPPSGDGEKPPKPKRFWLRFTLASVIIMAVS
                     AAAIAVSGLVYVDSIVAEIKPDKELSKAARFITNVNGGEPQNILIIGSDKRANEDEDP
                     GRSDTTILVRIDPDQEAISVLSIPRDLKVEIPGFGTEKFNAAYTWGGTALTVQVVKQL
                     TGLPIHHVVNVDFLGFVRAVYALGCVYTDVDRRYYHSNEGVPPSEQYAEINVQPGYQL
                     LCGRPALEYVRYRHTDTDIVRSARQQDFLSAARQRIDVGNLVDDVVFGAGENGLIEIA
                     TKYVSSDIDSKEELLDLLKLFVAARGASLHEVHFPAELGPSYVESTPEQINEAVNEFL
                     GVSESSDAPENQEGAEEKEQDEAAKSKKKGKDGKKKGKGKKKKKKKDKPIVEAPPAGS
                     DELVLAREAGEEEAKIAARRVHGQFPIFYPTRLPSGAYYVESNPYEKVVDPRIYRIPE
                     GLGKDSPRHEAYRMVLVMEQSDGTHYFGVQGIRGWSDPPILDNPSETKEINGREYEIF
                     ADSGRIKLIAWHRGDNSYWISNDLLQSLSNDQMVGMARSADVLVPKKKRKKGGSKG"
     gene            3622..>4082
                     /locus_tag="VFB52_13925"
     CDS             3622..>4082
                     /locus_tag="VFB52_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZO07483.1"
                     /translation="MSAQHEPVGVIGVGWVGLVTAVCFAELGHEVIARDILADKVESL
                     SRGETTIHEPGLEEMLRRNAERITFTTDMSELLGRARLLFVCVDTPPTYSGDADLSRV
                     RAVVQELPDDSEHVLIMKSTVPAGTGNSIRRDRPGLPYVSCPEFLKEGSAVA"
BASE COUNT          760 a         1379 c         1370 g          573 t
ORIGIN      
        1 agccgttctt cgtggccgag cagttcaccg gcctgtccgg caagtacgtg ccggtcgagg
       61 agacggtgcg ctcgttcaag gagatcatcg aggggaagca cgacgagatc cccgagcgcg
      121 cgttcctgct caagggcacg atcgacgacg tcgtgaactc ggtacgcggc agcgacgcca
      181 aggaggagaa gcaggaggac gagggtggcg aggaatagct tccccgtaga ggtcctcacg
      241 cccgagggcc aggtcttcgc cgacgaggtg gagatgctgt cgacgcgcac gacgatctct
      301 ccgagggtgc cttctacatg cagggctcga tcgaccaggt gctcgagaac gccaaggccg
      361 gcgccgccgc ctagcgggac cgggactgaa gacgatggcc gccgagcaca tgatcgacgt
      421 cgagatcctc acccccgagg gtgaggtctt cgagggcgaa gcgcgccagg tctcgacccg
      481 caccgaggtc ggcgaaatcg gcatcctcgc caaccacacg ccgctgctgg cggcgctgaa
      541 gccgaccgag ctgcgtctga aagtctccga ctcggagacc aagcgctacg cgcaggcgca
      601 cggctggctg caggtcttcg gcaaccgcgc ccgtctcctg gtcgaggagg cgatcgcgcc
      661 ggaggacctc gacgccggcg tgctgaaaga acagctcgcc gacgccgagc agcgcctctc
      721 cgccagcgac gaggacagcg ccgagcacgc ccgcgcgaaa aaagacctcg accgggccga
      781 agcgtttctc gctattacgg gttgaggcga gcaccctgtg ggtgctctcc gtgacaacct
      841 gcgccgatga ctgacgagct catctcgctc gcgcagcggc tgatcagctt tgagaccacc
      901 gaccccgacg ggatctccga ggcggccggc ttcgtccagg gctggctcgg cgcgcgtggg
      961 atcgaggcca agggcagcga ggtccgcggg ctgccggtgg tgatggccga ggtcggcccc
     1021 gccgacgccc cgaccgtggt cctgcacggc cacatcgacg tcgtgcccgg gcacgacgac
     1081 cagttcctgc cgcgggtcga gggcgacaag ctgatcgggc gcggcgccta cgacatgaag
     1141 ggcgcggtcg cggcgatgat gctggttgca gcctcgatgc gcgaccagga tcgggtccgg
     1201 gtgcggctcg ggatcgtcgg cgacgaggag tccgaggagg agaacgagcg cggttccgac
     1261 cacctcgtcg acagcggctt cctcggcgac ttcgcgatca ccggcgagcc gaccgacctg
     1321 cacatcggaa tcgaggccaa gggcgtgctg gcgctgcgcc tggaggtagg ggggagggcc
     1381 gcccacgggg cgaccccgtg gctgggcgac aacgccgtcc tcaacgcgat cgacgttttc
     1441 cgcagtatcg agtcgctacc gtttgcgcgg cacagttcgg agctgttcga ccgcccctcg
     1501 atcaacctgg gccggatctg gggtggggac gcgctgaaca aggtgcccga tcgatgcatc
     1561 atcgacgtcg acattcgcta cctgcccgaa caggaccccg ccgggatcct cgaggaagtg
     1621 cgcgccctgc cgggctgcga gccgacgccg ctgctgaccc ggccgccggc gacggtcgag
     1681 cgcaactcgc ccttcgtgct ggggctgcgc gaggcggcgc gggcgcatca cgacggcgag
     1741 ccgatgagcg tcgggcgcga cggcgcctcc gacgcggtct ccttcctgcg ggtcggggtg
     1801 ccggcggtcg agttcgggcc ggtcggtgcc ggccaccacg gtcccgagga gtgggtctcg
     1861 gtctcctcgc tggcgaccta ccggcaggcg ctcgaatcct tcttgatcgc gatcccggat
     1921 cggctcggcg atgagtgagc cactgggccc gggctgggcc ccgaagaagc cgccgccgag
     1981 cggcgacggc gagaagccgc cgaagccgaa gcgcttctgg ctgcgcttca cgctcgcctc
     2041 ggtgatcatc atggccgtct ccgccgcggc gatcgccgtc tcagggctgg tctacgtcga
     2101 cagcatcgtc gccgaaatca aacccgacaa ggaactcagc aaagccgccc gcttcatcac
     2161 caacgtgaac ggcggcgaac cgcagaacat cctgatcatc ggttccgaca agcgggccaa
     2221 cgaagacgaa gacccgggcc gttcggacac cacgatcctg gtccgcatcg accccgacca
     2281 ggaagcgatc tcggtgctct cgatcccgcg cgacctgaaa gtcgaaatcc ccggcttcgg
     2341 gaccgaaaag ttcaacgccg cctacacctg gggtgggacc gcactgacgg tccaagtggt
     2401 caagcagctg accgggctgc cgatccacca cgtcgtcaac gtcgacttcc tcggcttcgt
     2461 ccgcgccgtc tacgcgctcg gctgcgtcta caccgacgtc gaccgccgct attaccactc
     2521 caacgaaggc gtgccgccct ccgaacagta cgccgagatc aacgtccagc ccggctacca
     2581 gctgctctgc ggccggcccg cgctggagta cgtgcgctac cgccacaccg acacggacat
     2641 cgtccgctcg gcccgccagc aggacttcct cagcgccgcc cgccagcgca tcgacgtcgg
     2701 caacctggtc gacgacgtcg tcttcggggc cggggaaaac ggcctgatcg agatcgcgac
     2761 caaatacgtc tcctccgaca tcgacagcaa ggaggaactg ctcgacctgc tgaaactgtt
     2821 cgtcgccgcg cgcggcgcct cgctgcacga ggtccacttc ccggccgaac tcgggcccag
     2881 ctacgtcgaa tcgacgcccg aacagatcaa cgaagcggtg aacgaattcc tcggcgtctc
     2941 cgaaagcagc gacgcgccgg aaaaccagga aggcgccgaa gaaaaagaac aggacgaagc
     3001 cgccaagagc aagaagaagg gcaaggacgg caagaagaag ggcaagggca agaagaagaa
     3061 aaagaagaag gacaagccga tcgtcgaggc gccgccggct ggctccgacg aactcgtgct
     3121 cgcccgcgaa gcgggcgaag aggaggcgaa gatcgccgcc cgccgcgtcc acggtcagtt
     3181 cccgatcttc tacccgaccc gcctgccctc cggtgcctac tacgtcgaaa gcaaccccta
     3241 cgagaaagtc gtcgacccgc gcatctaccg gatcccggag gggctgggca aagactcgcc
     3301 gcgccacgag gcctaccgga tggttctggt gatggaacag tccgacggca cccattactt
     3361 cggcgtgcag ggcatccgcg gctggtcgga cccgccgatc ctcgacaacc cgagcgaaac
     3421 caaggagatc aacggccgcg agtatgagat cttcgccgac agcggccgga tcaaactgat
     3481 cgcctggcac cgcggcgaca acagctactg gatctcgaat gacctgttac agagtttgag
     3541 caacgaccag atggtcggga tggcgcgttc tgccgacgtg ctggtcccga agaagaagcg
     3601 gaagaaaggc ggaagcaaag gatgagcgcg cagcacgagc cggttggcgt gatcggggtg
     3661 ggctgggtcg gtctggtgac cgccgtctgc ttcgccgagc tgggccacga ggtgatcgcc
     3721 cgcgacatcc tcgccgacaa ggtcgagtcg ctctcgcgcg gcgagaccac gatccacgag
     3781 cccggcctcg aggagatgct gcgtcgcaac gccgagcgga tcaccttcac cacggacatg
     3841 tcggagctgc tcgggcgggc gcgcctgctc ttcgtctgcg tcgacacgcc gccgacctac
     3901 tcgggcgacg ccgacctctc gcgggtgcgg gccgtcgtcc aggagctgcc cgacgacagc
     3961 gagcacgtgc tgatcatgaa gagcacggtg ccggcgggga ccggcaactc gatccgccgt
     4021 gatcgtccgg gcttgcccta cgtctcctgt ccggagttcc tcaaggaggg ctcggcggtc
     4081 gc
//
LOCUS       DASRIG010000519         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2054458, whole genome shotgun sequence.
ACCESSION   DASRIG010000519 DASRIG010000000
VERSION     DASRIG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054458"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1950
                     /locus_tag="VFB52_13930"
     CDS             <1..1950
                     /locus_tag="VFB52_13930"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HZO07484.1"
                     /translation="LTAAGVNGPCVGVEGPSGESVFNVEADDVSIFRLAIGGGSFGIA
                     VINASEDFVLRSSWIGFNLEGGSAANNNIGVFIDPTSDGATIGGEGSIQRNVIGNSGV
                     GLDVEGADETTIQGNYFGVGPNGSTPATNNKDIEVTDSTSGAGFEAENTVIGATVSGG
                     SAETAACDGGCNVISGALAIGLDLAGNGSGQGEAPATGPTTVQGNYVGLNAAGTETVS
                     DAQFGILAGGAGDALVGGPVPGAANFIAGGTFGVYGENGDDLEVAGNVIGIEPTGEFS
                     SAPAEVGIFVFALTLSTPEAGATVSTNTVRMAPGSGVGIEHRFSGAKILGNVVEGGAT
                     GILTKGSANGSQIEDNTVVASEDSGIVLLNPDNEVFGNEVVESGEAGIEVTPGAAIDV
                     SGNVIGGDSAADENAIFDSGGGAIEIFGIEESRNEARRNRGDGNGEGFIRVRGYEGSE
                     PGNGVESPEISAAGKTETSGTAEPGAVVRVFSKASAEEGEIAGFVGQATANGSGQWTA
                     TYAGLPGGTVVTATQTLDGGTSGLSETAKTPPDPPAPPVCPQAGATNCGPPPPPPVTK
                     PKVTIKKAPKAKSKSTTAKFVFKSSVAGSKFKCKLDKKAFANCRSPKTYKKLKPGKHV
                     FKVKATNSAGTSPVVTRKFTVLPPE"
     gene            1964..4303
                     /locus_tag="VFB52_13935"
     CDS             1964..4303
                     /locus_tag="VFB52_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HZO07485.1"
                     /translation="MLVARARSRGILAAVFAVLGCALILLLTPGSAGAVTYTVNSIGD
                     QPDENPGAEGCKTTVANCTLRAAIQESNFSTSVDDVIQFSALFNGETLGTISIGGGFP
                     VITDSVVIAGDASGPASGRCATSAGVEGPCVGVSGEPGLDVQASGTTISGLAVFNATI
                     GIAVEADGVALRDNWIGIKLDGTAGTTAPNVGIFVAPGADNAQVGGVLTADRNVIGNT
                     TSGLVLRGASLAQVQGNYFGVRPDGTTVAPNSRDLVVANKIGAPDVPATDNLIGANVG
                     AGGAETPACDFGCNVFASSASSDAAIDLLGQTILEEKSATGPTTILGNYLGLDAAGQP
                     FASAATSAIRVGSAGDVTIGGLQPGEANQIHGGGYGVFAGNLGVPAKELQVLGNMIGR
                     ALDNSDFLFPPGEGISLSSAGIAVTPDASIVEGNYISATGNGIENHSAGATIAANHIY
                     GGTRGIWTKGSTEESGIGNLIKGNTITDALEYGVFLQNDFNVVIDNYVSGSEVGIGIG
                     NFLTLDSTGNLIGGDGDGEDNLIFESASDAIEVVDVEGSQNEIGRNGGSGNGGLFIDL
                     RAAEPGTEPNGPNGGIKPPVVTTASKTGASGTAEAGATVRVFAKAGSEPGELGDYLGK
                     ASAGIGGEWSLVFLNPVAVGSLVAATQTNTEGGTSEVSATATVPPDQQTGCEVVVPKP
                     PSCLPPPPPDTTRPNVTIEKAPKAKSTSTTAKFKFVSNEAGSKFKCKLDKKAFANCRS
                     PKTYKKLKLGKHVFKVKATDAAGNVSAVVTKKFTVLPPS"
     gene            4452..>5262
                     /locus_tag="VFB52_13940"
     CDS             4452..>5262
                     /locus_tag="VFB52_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07486.1"
                     /translation="MTFTVDSTGDGGTQATCEEQSPGCTLRGAIQAANATDAAPDVIA
                     FGAVFNGEIGDTIEIGTPLPPITSPVAIDGKTCATAAAVQGPCVGVNAVAGAAWTFDV
                     EEDDTTIDNLAISGGTSPILVIEASTSFSAAGNWIGVKLNGSAAGASQAGILIGQGSD
                     GATIGGVEPGEGNVIANNSTGLAVYGAAGTVVQGNWFGVGPAGTAATAKAGVDIEVRD
                     LGEAKATGTEIGATITGVALTTKACDGGCNVISHASLSGIDLSFGASGPTTI"
BASE COUNT          995 a         1854 c         1724 g          689 t
ORIGIN      
        1 ctcactgccg ccggggtcaa cggtccctgt gtcggggtcg aaggtccgag cggcgaatcg
       61 gtcttcaacg tcgaagccga cgacgtctcg atcttcagac tcgcgatcgg gggcggctcg
      121 ttcgggatcg cggtgatcaa cgcgagcgaa gacttcgtcc tgcgcagcag ctggatcggc
      181 ttcaacctcg aaggcggctc ggcggcgaac aacaacatcg gggtcttcat tgacccgacc
      241 tccgacggcg cgacgatcgg tggcgaaggg agcatccagc gcaacgtgat cggcaacagc
      301 ggcgtcggcc tcgacgtcga aggcgccgac gaaacgacga tccagggcaa ctacttcggg
      361 gtcgggccga acgggtccac ccccgcgacg aacaacaagg acatcgaggt caccgactcg
      421 acctcgggtg cgggcttcga ggccgaaaac accgtgatcg gcgccacggt cagcggcgga
      481 tcggccgaaa ccgcggcctg cgacggcggc tgcaacgtga tctccggtgc gctcgcaatc
      541 gggctcgacc tcgccggcaa cggctccggc cagggcgagg cgcccgccac gggcccgacc
      601 acggttcagg gcaactacgt cggcctcaac gcggcgggga ccgaaacggt ttccgacgcc
      661 cagttcggga tcctcgccgg gggtgccgga gacgccctcg tcggcggccc ggtgcccggc
      721 gccgcgaact tcatcgccgg cggcaccttc ggcgtgtatg gcgaaaacgg cgacgacctc
      781 gaagtcgccg gcaacgtgat cgggatcgag ccgaccggcg aattctcgtc tgcgcccgcg
      841 gaagtcggga tcttcgtctt cgcgctcacg ctcagcaccc cggaagccgg tgccaccgtc
      901 agcacgaaca cggtgcggat ggctcccggc agcggggtcg gcatcgaaca ccgcttcagc
      961 ggcgcgaaga tcctcggcaa cgtcgtcgaa gggggcgcca ccgggatcct caccaaaggc
     1021 agcgccaacg gcagtcagat cgaagacaac accgtggtgg ctagtgaaga ctccgggatc
     1081 gtcctgctga accccgacaa cgaagtcttc ggcaacgaag tcgtcgaatc cggcgaggcg
     1141 gggatcgaag tcaccccggg cgccgcaatc gacgtcagcg gcaacgtgat cggcggcgat
     1201 tcggccgccg acgaaaacgc gatcttcgac tccggcggcg gcgcgatcga aatcttcggg
     1261 atcgaagaaa gccgcaacga agcccgccgc aaccgcggcg acggcaacgg tgagggcttc
     1321 atccgcgtgc gtggctacga agggtccgag ccagggaacg gcgtcgagtc gccggagatc
     1381 agcgccgccg gcaagaccga aacgagcggg accgcggaac ccggcgcggt ggttcgcgtc
     1441 ttcagcaagg cgagcgccga agaaggcgag atcgccggct tcgtcggcca ggcgaccgcc
     1501 aacggcagcg gccagtggac cgcgacctac gcgggcctgc ccggcggcac cgtcgtgacc
     1561 gcgacccaga ccctcgacgg cgggacctcg ggcctgagcg aaacggcgaa aacgccgccc
     1621 gatccgccgg ccccgcccgt ctgcccgcag gccggcgcca ccaactgtgg cccgccgccc
     1681 ccgcccccgg tcaccaagcc gaaggtgacg atcaagaagg ctccgaaggc caaatccaag
     1741 agcacgaccg cgaagttcgt gttcaagtcc tccgtcgccg gctcgaagtt caagtgcaag
     1801 ctcgacaaga aggccttcgc caactgccgc tcaccgaaga cctacaagaa gctgaagccg
     1861 ggcaaacacg tcttcaaggt caaagcgacc aactcggcgg gcacgagccc ggtcgtcacg
     1921 cgcaagttca ccgtattgcc gccggagtaa taaagtttct gcagtgctcg tcgctcgcgc
     1981 acgttcgagg gggatactcg ccgccgtctt cgcggtgctc ggctgtgcgc tgatcctcct
     2041 actgaccccg ggtagcgctg gggcggtcac ctacacggtc aactcgatcg gcgaccagcc
     2101 cgacgaaaac cccggcgcgg aagggtgcaa aaccaccgtc gccaactgca ctctgcgggc
     2161 ggctatccag gagtcgaact tctcgaccag cgtcgacgac gtgatccagt tcagcgcgct
     2221 gttcaacggg gaaacgctcg gcacgatcag catcggcggc ggcttcccgg tgatcaccga
     2281 ctcggttgtg atcgccggcg acgcgtccgg cccggcttcc gggcgctgcg ccaccagcgc
     2341 cggggtcgaa gggccctgtg tcggcgtcag cggtgaaccg ggcctcgacg tgcaggcgag
     2401 cgggacgacg atcagcggcc tggccgtgtt caacgcgacg atcgggatcg cggtcgaagc
     2461 cgacggcgtc gccctcagag acaactggat cgggatcaag ctcgacggca ccgccggcac
     2521 gaccgcgccg aacgtcggga tcttcgtcgc gcccggcgcc gacaacgccc aggtcggcgg
     2581 tgtcctcacc gccgaccgca acgtgatcgg caacaccacc agcggcctgg tcctgcgcgg
     2641 ggccagcctg gcgcaggttc agggcaacta cttcggcgtc cggccggacg ggacgacggt
     2701 cgcgccgaac tcgcgcgacc tcgtcgtcgc caacaagatc ggcgcccccg acgtccccgc
     2761 caccgacaac ctgatcggcg ccaacgtcgg cgcgggtggc gcggaaaccc ccgcctgcga
     2821 cttcggctgc aacgtgttcg ccagcagcgc cagctcggat gccgcgatcg acctgctcgg
     2881 gcagacgatc ctcgaagaga aatcggcgac cgggccgacc acgatcctcg gcaactacct
     2941 tggcctcgac gccgccgggc agccgttcgc ctccgcggca acctctgcga ttcgggtcgg
     3001 cagcgccggc gacgtgacga tcggcggact ccaacctggt gaggccaacc agatccacgg
     3061 cggcggctac ggggtcttcg ccggcaacct gggggtgccc gcgaaagagc tgcaggtgct
     3121 cggcaacatg atcggccgcg ccctcgacaa ctcggacttc ctcttcccgc ccggcgaagg
     3181 gatctcgctc tcctcggccg ggatcgcggt cacgcccgac gcctcgatcg tcgaaggcaa
     3241 ctacatctcc gcaaccggga acgggatcga aaaccactcg gccggggcga cgatcgccgc
     3301 caaccacatc tacggcggca ccaggggcat ctggaccaaa ggcagcaccg aagaaagcgg
     3361 aatcggcaac ctgatcaaag gcaacacgat caccgacgcg ctcgaatacg gggtcttcct
     3421 ccagaacgac ttcaacgtcg tgatcgacaa ctacgtctcc ggcagcgaag tcgggatcgg
     3481 gatcgggaac ttcctcacgc tcgactcgac cggcaacctg atcggcggcg atggcgacgg
     3541 cgaagacaac ctgatcttcg aaagcgcctc cgacgcgatc gaggtcgtcg acgtcgaagg
     3601 ctcccagaac gaaatcggcc gtaacggcgg ctccggcaac ggcggcctgt tcatcgacct
     3661 gcgcgcggcc gaacccggaa ccgaaccgaa cgggccgaac ggcgggatca aaccgccggt
     3721 ggtgacgacg gcgagcaaaa ctggggccag cggtaccgca gaggctggag ccacggtccg
     3781 ggttttcgcc aaggccggct ccgaaccggg tgagctcggc gattacctcg gcaaggcatc
     3841 cgccgggatc ggcggcgaat ggagcctcgt cttcctcaac ccggtcgcgg tcggctcgct
     3901 ggtcgccgcc acgcagacca acaccgaagg tggcacctcg gaggtcagcg cgaccgcaac
     3961 cgtgccgccc gaccagcaga ccggttgcga agtggtcgtg ccgaagccgc cctcctgcct
     4021 gcccccgccg ccccccgaca ccaccagacc caatgtgacg atcgagaagg cgccgaaggc
     4081 caaatcgacg agcaccaccg caaagttcaa gttcgtctcc aacgaggccg gctcgaagtt
     4141 caagtgcaag ctcgacaaga aggccttcgc caactgccgc tcgccgaaga cctacaagaa
     4201 gctgaagctc ggcaagcacg ttttcaaggt gaaagcgacc gacgcggccg gcaacgtcag
     4261 cgccgtggtc accaagaagt tcaccgttct gccgccgagc tgagcccgac gattcgcgga
     4321 ttgtggaacg atcctgtagg gtcgctcccg tgctcgttgt tcgtcccggc cagggcgtaa
     4381 tccgtaccgc cctcaccgcg acgctcctcg ctgcgctgat ttcgctggcc ttggcggcct
     4441 ccgcctctgc cgtgaccttc acggttgaca gcaccggcga cggggggacc caggcgacct
     4501 gtgaagagca gtcccccggc tgcaccctgc gcggcgcgat ccaggcggcc aacgccaccg
     4561 acgcggcacc ggacgtgatc gccttcggcg ccgtcttcaa cggcgagatc ggcgacacga
     4621 tcgagatcgg cacgccgttg ccgccgatca cctcgcccgt cgcgatcgac ggcaaaacct
     4681 gcgccaccgc cgcggccgtc caaggcccct gcgtgggcgt caacgccgtc gcgggcgcgg
     4741 cctggacctt cgacgtcgaa gaggatgaca cgacgatcga caacctggcg atctccggcg
     4801 gcaccagccc gatcctcgtg atcgaagcca gcaccagctt cagcgccgcc ggcaactgga
     4861 tcggcgtcaa actcaacggc agcgccgccg gcgccagcca ggcgggcatc ctgatcggcc
     4921 agggctcgga cggcgcgacg atcggcgggg tcgaacccgg ggaaggcaac gtgatcgcca
     4981 acaactcgac cgggctggcc gtctacggcg ccgccggcac ggtcgtgcag ggcaactggt
     5041 tcggagtcgg ccccgccggc accgcggcga cggccaaagc cggagtcgac atcgaagtcc
     5101 gggacctggg cgaagcgaaa gcgaccggca ccgagatcgg cgcgacgatc acgggtgtgg
     5161 cgctgaccac gaaggcctgc gacggcggct gcaacgtgat ctcccacgcc tcgttgtccg
     5221 ggatcgacct cagcttcggc gccagcggcc cgaccacgat cc
//
LOCUS       DASRIG010000520         4435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3345821, whole genome shotgun sequence.
ACCESSION   DASRIG010000520 DASRIG010000000
VERSION     DASRIG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345821"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFB52_13945"
     CDS             <1..488
                     /locus_tag="VFB52_13945"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZO07487.1"
                     /translation="VHNAGGAVDVHLMIETPERQIEDFAKAGADSISFHDEATPHTNR
                     TLHAVRELGCLAGIAINPGTPPESLSELRGLADVVLCMSVNPGWGGQSFIEASPAKVE
                     RLAALVGDAKIEVDGGVDVATAGSVAAAGASMFVAGSAVFGAADPAQAYRDIAQAAGA
                     I"
     gene            complement(485..2278)
                     /locus_tag="VFB52_13950"
     CDS             complement(485..2278)
                     /locus_tag="VFB52_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HZO07488.1"
                     /translation="MGAHDAELILIAGVLLAAGIAGVLFADRVRVPSLLLFLGLGILA
                     GSEGIGEIDFSNAELARTLGTIALVLILFEGGLTAGWSEIRPVLGTAASLATIGTLVT
                     AVIAGFGAMLIFDLSTLEGLLVGAAIAATDSAAIFAVLRNSTVEKRLARSLEGESGMN
                     DPVALLLVIGFIEWIQEPGYGLADMVGLLAVKLAVGIVIGVAIGKLAALAMERVKLPS
                     DGLYPVATMAIAGLSYGIAEAAHGSGFLAIYLTALALGSARLPARRTLIAFHEGLGWI
                     AQISLFIMLGLLIFPATLDDVALEGLALSAVLIFLARPVATFVATALAPFNSRERLML
                     GWAGLRGATPIWLATFPLVAGVAGGQELFSIVFFVVVSSTLIQGASFEPLATRLGLTT
                     SEPALPRRMLATGRIREMGGEVISFKLPPGAAAIGHPVRDLALPREALVNVIVREGNA
                     IPPRGSTELREGDELHVLARSELRDEVEALTEVWSEGPVGKPAAPKLPRRGASQVFSV
                     RPARQGEDPLTLDKVSGIPVIAVMRTRNDRPGAMVALADGRYAVTGEVVAIGGRRALA
                     DWCERRAAREGVNAAERAWWQEVTGALIATG"
     gene            complement(2330..3730)
                     /locus_tag="VFB52_13955"
     CDS             complement(2330..3730)
                     /locus_tag="VFB52_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07489.1"
                     /translation="MSRFRRGAAQGETASSAEYGKTASFLAIGVGLTGLITYAYFLIA
                     SHTLSKLDYGQITILWSAVFITISTLYRPVEQLLSRHISQRLVNGESLSQPMRVASTI
                     QVGLALIFAVGALALRGPIEGDLLDGNEPLYWVFFGAVLFYAASYFARGYLAGRREFG
                     PFTALILSESIFRTSFAVLVAVGILSGQDMVAVGIVAAPSLSLIVVPIFFAWRAKKAK
                     DEPPGRVAEDAPDESFSMKEGSGFAAAVLVIMFSEQAFLNAGPLIIGALSGATATGFI
                     FNMLMIARAPLQLFQAVSTSILPHLTSLHTSSEPDSDREFHRSVRGVLLGIAGFASFT
                     AVVVLIAGPKLMEIAFSGKYTYDRPGLLLVTLGMGLYLGAVTLNQACLAQGQVRRAAA
                     RWIGCAAFFIAWTIVPLISDEFRRVEIGFALTAGILFTLLYLVYRHPHERPEDVPEPG
                     SPEELEARLASIDENV"
     gene            complement(3730..>4435)
                     /locus_tag="VFB52_13960"
     CDS             complement(3730..>4435)
                     /locus_tag="VFB52_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO07490.1"
                     /translation="ADVAKLVAPIIAGEADMVVGDRQVGSIDHFSGSKKGLQRLGSWV
                     VRRVSGTDVTDATSGFRAYNREAALQLAVVDNFTYTLESLIQAGKMLVAVDEVPVGTN
                     PQTRESRLFNSTGGYVRRNGLAILRIYARYEPLRAFASAGAIVLLLALAAWMPFLLDW
                     IFNGDSSGHVQSLILGAVLFIAAIQLFALGVIGDLLASQRVMTQRVFERVRRVELAVG
                     VEPSHYERGERTPQDD"
BASE COUNT          729 a         1527 c         1594 g          585 t
ORIGIN      
        1 aggtccacaa cgccggcggt gccgtcgacg tgcacctgat gatcgagacg cccgagcgcc
       61 agatcgagga cttcgccaag gccggcgccg actcgatcag cttccacgac gaggcgaccc
      121 cgcacacgaa tcgcaccctg cacgccgtgc gtgagctcgg ctgcctggcc gggatcgcga
      181 tcaaccccgg taccccgccc gagtcgctca gcgagctgcg cggcctcgcc gacgtcgtcc
      241 tctgcatgag cgtcaaccct ggctggggcg gtcagtcctt catcgaggcc tcgccggcca
      301 aggtcgaacg cctcgccgcg ctggtcggcg acgccaagat cgaggtcgac ggcggcgtcg
      361 acgtcgccac cgccggctcg gtcgccgccg ccggcgcctc gatgttcgtc gccggctccg
      421 ccgtcttcgg cgccgccgac ccggcccagg cctaccgcga catcgcccag gccgcgggcg
      481 cgatctagcc ggtcgcgatt agcgcgcccg ttacctcctg ccaccaggcg cgttccgccg
      541 cgttcactcc ctcgcgcgcc gcgcggcgct cgcaccagtc agccagcgcc cggcggccgc
      601 cgatcgcgac gacctcgccg gtcaccgcgt agcggccgtc ggcgagcgcg accatcgccc
      661 cggggcggtc gttgcgggtg cgcatcaccg cgatcaccgg gatgccgctg accttgtcga
      721 gcgtcagcgg gtcctcacct tggcgggcgg ggcgcaccga gaagacctgg gaagcgccgc
      781 gccgcggcag cttcggcgcc gccggtttgc cgaccgggcc ttcgctccaa acctcggtca
      841 gcgcctcgac ctcgtcgcgc agctcgctgc gcgcgagcac gtgcagctcg tcgccctcgc
      901 gcagctcggt cgagccgcgt ggcgggatcg cgttgccctc gcgcacgatc acgttgacca
      961 gcgcctcgcg cggcaacgcc aggtcgcgca cgggatggcc gatcgcggcc gctcccgggg
     1021 gcagcttgaa ggagatcacc tcgccgccca tctcgcggat ccgccccgtc gcgagcatgc
     1081 gccgcggcag cgccggttcg ctcgtggtca ggccgagccg ggtggcgagc ggctcgaagc
     1141 tggctccctg aatcagggtc gagctgacga cgacgaagaa gacgatcgag aacagttcct
     1201 ggccgccggc gaccccggct acgagcggga aggtggcgag ccagatcggc gtcgccccgc
     1261 gcagcccggc ccagccgagc atcaggcgct cgcgggagtt gaacggcgcc agcgccgtcg
     1321 ccacgaaggt cgcaaccggc cgggcgagga agatcagcac cgccgagagg gccaggccct
     1381 cgagcgcgac gtcgtcgagc gtggccggga agatcagcag cccgagcatg atgaagagcg
     1441 agatctgggc gatccagccg agcccttcgt ggaaggcgat caaggtgcgg cgggcgggca
     1501 ggcgggccga gccgagggcg agcgcggtca ggtagatcgc gaggaagccg gaaccgtggg
     1561 cggcctcggc gatcccgtag gagaggccgg cgatcgccat cgtcgccacc gggtagagcc
     1621 cgtccgaggg cagtttcacc cgttccatgg caagcgccgc gagtttgccg atcgcgacgc
     1681 cgatcacgat cccgaccgcg agtttcaccg ccagcagccc gaccatgtcg gcgagtccgt
     1741 agccgggctc ctggatccat tcgatgaacc cgatcacgag cagcagggcg accgggtcgt
     1801 tcatcccgga ctcgccctcg agcgagcgcg ccaggcgctt ctcgacggtc gagttgcgca
     1861 gcaccgcgaa gatcgcggcc gagtcggtcg cggcgatcgc cgccccgacc agcagccctt
     1921 ccagggtcga gaggtcgaag atcagcatcg ctccgaagcc ggcgatcacc gccgtgacca
     1981 gggtcccgat cgtcgccagc gaggcggcgg tgccgagcac gggccggatc tccgaccagc
     2041 ccgcggtcag gccgccctcg aagaggatca ggaccagcgc gatcgtgccc agggtgcggg
     2101 ccagctccgc gttgctgaag tcgatctcgc cgatcccttc ggagccggcg aggatcccga
     2161 ggccgaggaa gagcagcagg ctggggaccc gcacgcggtc ggcgaagagg actccagcga
     2221 tgcccgccgc cagcaggacg ccggcaatca ggatcagctc ggcgtcgtgt gcacccatta
     2281 agggacccta atgaaccgta agtggtttac gccgaccggt accgcgggtc tagacgttct
     2341 cgtcgatcga ggcgagccgc gcctccagct cctccggcga gcccggctcg ggcacgtcct
     2401 cgggccgctc gtgcgggtgg cggtagacga ggtagagcag ggtgaagagg ataccggcgg
     2461 tcagcgcgaa gccgatctcg acccggcgga actcgtcgga gatcagcggg acgatggtcc
     2521 aggcgatgaa gaaagccgcg cagccgatcc aacgcgccgc ggcccgccgc acctggccct
     2581 gcgccaggca ggcctggttg agggtgaccg cgccgaggta gaggcccatg cccagcgtca
     2641 ccagcagcag cccggggcgg tcgtaggtgt acttgccgct gaaggcgatc tccatcagct
     2701 tcgggccggc gatcaggacg acgaccgcgg tgaagctggc gaagccggcg atcccgagta
     2761 ggacgccgcg gaccgagcgg tggaactcgc ggtcggagtc gggctcggac gaggtgtgga
     2821 ggctggtcag gtggggcagg atgctggtcg agaccgcctg gaagagctgc agcggcgccc
     2881 gcgcgatcat cagcatgttg aagatgaagc cggtcgcggt cgcgcccgag agggcgccga
     2941 tgatcagggg cccggcgttg aggaatgcct gttcgctgaa catgatcacc agcaccgctg
     3001 cggcgaaccc ggagccctcc ttcatcgaga aggactcgtc cggcgcgtcc tctgccacgc
     3061 ggccaggtgg ttcgtccttc gctttcttcg cccgccaggc gaagaagatc gggaccacaa
     3121 tcagcgaaag ggagggcgcg gcgacgattc ccaccgcgac catgtcctgg ccgctgagaa
     3181 tcccgactgc gaccagcacc gcgaacgagg tgcggaagat cgactcggag aggatcagcg
     3241 cggtgaaggg gccgaactcc cggcggccgg cgaggtagcc gcgggcgaag taggaggcgg
     3301 cgtagaagag gacggcgccg aaaaagaccc agtagagcgg ttcgttgccg tccagcagat
     3361 cgccttcgat cggcccgcgc agggcaagcg cccccaccgc gaagatcagc gccagcccga
     3421 cctggatcgt cgaggcgacc cgcatcggct gactgagcga ttcgccgttg accaggcgct
     3481 gggagatgtg ccgggagagc agctgttcga ccgggcggta gagggtcgag atcgtgatga
     3541 agaccgccga ccagaggatc gtgatctgcc cgtagtcgag cttcgacagg gtgtgggagg
     3601 cgatcaggaa gtaggcgtag gtgatcaggc cggtgaggcc gaccccgatc gccaggaagc
     3661 tcgccgtttt gccgtactcc gcgctggagg cggtctcgcc ctgcgcggcg ccgcgccgaa
     3721 acctcgacat cagtcgtcct ggggggtgcg ctcgccgcgc tcgtagtgcg agggctcgac
     3781 cccgaccgcc agctcgaccc tccgcacccg ctcgaagacc cgctgggtca tcacccgctg
     3841 gctcgccagc aggtcgccga tcacgccgag cgcgaacagc tggatcgcgg cgatgaagag
     3901 cacggcgccg aggatcagcg actgcacgtg gccgctggag tcgccgttga agatccagtc
     3961 gagcaggaac ggcatccacg cggccagggc cagcagcagc acgatcgcgc cggcgctggc
     4021 gaaggcacgc agcggctcgt agcgcgcgta gatgcggagg atcgcaagcc cgttgcggcg
     4081 cacgtagccg ccggtcgagt tgaacagccg cgactcgcgc gtctgcgggt tggtcccgac
     4141 cgggacctcg tcgaccgcga ccagcatttt cccggcctgg atcaggctct ccagcgtgta
     4201 ggtgaagttg tcgacgaccg cgagctgcag ggcggcctcg cggttgtagg cgcggaagcc
     4261 ggaggtggcg tcggtgacgt cggtgcccga gacccggcgc acgacccagc tgccgagccg
     4321 ctgtagccct ttcttggagc cggagaagtg gtcgatcgag ccgacctggc ggtcgccgac
     4381 gaccatgtcg gcctcgccgg cgatgatcgg cgcgaccagc ttggcgacgt cggcg
//
LOCUS       DASRIG010000521         5255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2570760, whole genome shotgun sequence.
ACCESSION   DASRIG010000521 DASRIG010000000
VERSION     DASRIG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570760"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1321)
                     /locus_tag="VFB52_13965"
     CDS             complement(23..1321)
                     /locus_tag="VFB52_13965"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZO07491.1"
                     /translation="MRDDASVEAALPGEAAVAEAARRSLEGESKGIGRIWPFLGPAFV
                     AAVAYIDPGNFATNIAGGAEFGYLLLWVVLAANLIAMLVQAQSAKLGIATGRNLAELC
                     RERFSRRTSIGLWLQAELVAMATDIAEVVGAALGLYLLFGIPLFPAGIIAGAGAFAIL
                     ALQQMGYRRLEAGISVLVGIVVASFIYQLIDSSPDGGEVARHMFVPGFEGTESILLAT
                     GIIGATVMPHVVYLHSALTQRRIVGRTTAEKKKILGFEKVDVVIALSLAGLVNLSMMI
                     VAAALFHESGLTGVDSIEGAYEGLQSLVSQQAATIFGIALLASGFASSSVGTMAGQVV
                     MQGFIRRRIPLFLRRAITLTPALLVLAIGLNPTDALVGSQVVLSFGIPFALVPLLIIA
                     SKREVMGDLVNPRWLTGFAGVLASLIIALNVFLLEQVFFG"
     gene            complement(1318..1797)
                     /locus_tag="VFB52_13970"
     CDS             complement(1318..1797)
                     /locus_tag="VFB52_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZO07492.1"
                     /translation="MSAPEQEPGLRNSPSTEAIEDYAKAIYSISQERDGLVLNGEVAH
                     RLKVTPATATAMLKRLADLGLVDYKPYKGVSLTPAGERVALEVIRHHRLIELYLSEAL
                     GMDPDRVHEEAEVLEHHISEEFEALIAAKLGEPSHDPHGSPIPGPDLQPPSGERRAV"
     gene            complement(1797..3068)
                     /locus_tag="VFB52_13975"
     CDS             complement(1797..3068)
                     /locus_tag="VFB52_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZO07493.1"
                     /translation="MEAGSRAERPVGSGLPSWALGLIPLLLIAVAIGTFAALGGPGLG
                     DRNGPPVEEVSVESTVLHPGQIELTLQNDGPDPVEIAQVAVNDGFAPFTADGGREIGR
                     LGSTTLDITYPWIEGEAYDIFILTAVGGTITAEIPVAAETPDADLDFYGLMALLGIYV
                     GVIPVSLGMLWLPFLRRLGSQWMAALLAFTVGLLAFLAIDATLEGLEIAAEAPAAFGG
                     ASLVFAGALVAYLVLAGIDAWLERRAERATAAPDATQGGASDVGVAGVAAGGAYLSLL
                     VAIGIGLHNLGEGLAIGTSYATGALALGAFLVVGFAIHNTTEGLAIVAPLRKGGGEGE
                     RGLGLWRLLGLGVIAGAPAILGAWIGASAFNPSLAALLFGVGVGAIARVIVQIAPSMR
                     DEHGRLLYPATVAGMLVGIAALYLTGLLVTV"
     gene            complement(3068..4216)
                     /locus_tag="VFB52_13980"
     CDS             complement(3068..4216)
                     /locus_tag="VFB52_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZO07494.1"
                     /translation="MAPDSTKNGISRRRLLGGAAALSVPAAIGLNGLVNGNSGAALAT
                     NGEAATESPLQGHEHGGFIHAGFAPDRVVDHDRNGFNPTEILRDFDYGKTRRLPSGRV
                     LREWEIVAADREIEVAPGVSYEAWTYNGRVPGPTLRAREGELLRIKFINGSEHPHTMH
                     FHGVHTALMDGMPGIGEDRGGGQIEPGEAFTYEFDASPFGVHLYHCHVAPLAAHIARG
                     LYGAFIIDPKEGRPEADELVMVMNGFDTNFDGENEVYAVNTVGFHYINEPIAVPRDEL
                     VRIYLVNVLEYDPVNSFHIHGNFFHHYPTGTSLKPIEFTDTIIQGQAQRAILELEFPF
                     DGDYMFHAHVTEFAELGWTGFFRVGDPSKLTAAAAASMYCDLPWGAKA"
     gene            4324..>5255
                     /locus_tag="VFB52_13985"
     CDS             4324..>5255
                     /locus_tag="VFB52_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZO07495.1"
                     /translation="MATETEIELDAQYRQLREECGLLERSERSELLVSGADAAEYLQG
                     QLTNDTEALEPGDGLYAALLDRKGHMQSDMRVLRPGAGPDLLLDLETSALPAVLKHLT
                     MYSIGRDVAVADASEERALFSLIGPRSVELAGTAALPEDACEETSVAGVEVVAAGTRE
                     GIDLFCAATEAERLRAALLEAGAVAVSPTAAEILRIESGVPRYGAEMGGETMPAEAGI
                     VERAVSFTKGCYIGQETVARLHYKGKPNRHLRGLRLTDPVTQGDEIRLGERVVGEIGS
                     AAVSPAFGPIALAVVRREAEPGTVVSVGPSDVDG"
BASE COUNT          812 a         1766 c         1922 g          755 t
ORIGIN      
        1 cctgcgcttc atatctcctc cctcatccga agaacacctg ctctaacaag aagacgttga
       61 gggcgatgat caggctcgcg agcacgccgg caaagccggt gagccagcgc gggttgacca
      121 ggtcgcccat cacctcgcgc ttgctggcga tgatcagcag cgggacgagg gcgaagggga
      181 tcccgaacga gagcacgacc tggctgccga ccagggcgtc ggtcgggttg agcccgatcg
      241 ccagcaccag cagggccggg gtgagggtga tcgcgcggcg caggaagagc gggatgcggc
      301 ggcggatgaa gccctgcatc accacctgcc cggccatcgt cccgaccgag gaggaggcga
      361 agccggaggc gagcagggcg atcccgaaga tcgtcgccgc ctgctgggag accagcgact
      421 gcagcccttc gtaagcgcct tcgatcgagt cgaccccggt caggccgctc tcgtggaaga
      481 gggcggcggc gacgatcatc atcgagaggt tgaccaggcc ggcgagcgag agcgcgatca
      541 ccacgtcgac cttctcgaag ccgaggatct tctttttctc cgcggtcgtg cggccgacga
      601 tccgccgctg ggtcagcgcc gagtgcaggt agacgacgtg cggcatcacc gtcgcgccga
      661 tgatcccggt cgcgagcagg atgctctcgg ttccttcgaa gccggggacg aacatgtggc
      721 gggcgacttc gccgccgtcg gggctggagt cgatcagctg gtagatgaac gaggcgacga
      781 cgatcccgac caggaccgag atcccggcct ccagccgccg gtagcccatc tgctgcaggg
      841 cgaggatcgc gaaggcgccg gccccggcga tgatcccggc cgggaacagc gggatcccga
      901 acagcaggta gagcccgagc gcggcgccga ccacctcggc gatgtcggtc gccatcgcga
      961 ccagctcggc ctgcagccag agaccgatcg aggtgcggcg ggagaagcgc tcgcggcaga
     1021 gctcggccag gttgcgaccg gtcgcgatcc cgagcttcgc cgactgggcc tggaccagca
     1081 tcgcgatcag gttcgccgcc agcaccaccc agagcagcag gtagccgaat tcggcaccgc
     1141 cggcgatgtt ggtggcgaag ttgccggggt cgatgtaggc gaccgcggcg acgaaggccg
     1201 ggccgaggaa gggccagatc cggccgatcc ccttgctctc gccttccagg gagcggcgcg
     1261 ccgcctcggc gaccgcggcc tcgccgggaa gcgcggcctc gacgctcgcg tcgtccctca
     1321 gaccgccctc cgctcgccgc tcggcggctg caggtcgggg ccggggatcg ggctgccgtg
     1381 cgggtcgtgg ctcggttcgc cgagcttcgc cgcgatcagc gcctcgaact cctcggagat
     1441 gtggtgctcg agcacctcgg cctcctcgtg cacgcggtcg gggtccatgc cgagcgcctc
     1501 ggagaggtag agctcgatca gccggtggtg gcggatcacc tccagcgcca cccgctcccc
     1561 cgccggggtg aggctgacgc ccttgtaggg cttgtagtcg accaggccga ggtcggcgag
     1621 gcgcttcagc atcgcggtcg cggtcgcggg cgtgactttc agccggtgcg cgacctcgcc
     1681 gttgaggacc agcccgtcgc gctcctggga gatcgagtag atcgccttcg cgtagtcctc
     1741 gatcgcctcg gtgctgggcg agttgcgcag gccgggctct tgctcggggg cgctcatcag
     1801 acggttacca gcagaccggt gaggtagagg gcggcgatcc cgaccagcat cccggcgacg
     1861 gtggccgggt agagcaggcg gccgtgctcg tcgcgcatcg agggggcgat ctggacgatc
     1921 acccgggcga tcgcgccgac gccgaccccg aacagcagcg cggccaggct cgggttgaag
     1981 gcggaggcgc cgatccaggc gccgaggatc gccggcgcgc cggcgatcac gccgaggccg
     2041 agcaggcgcc agaggccgag cccgcgttcg ccttctcccc cgcccttgcg cagcggcgcg
     2101 acgatcgcga ggccctcggt ggtgttgtgg atcgcgaagc ccacgaccag gaatgccccc
     2161 agcgccagcg ccccggtcgc ataggaggtc ccgatcgcca ggccctcgcc gaggttgtgc
     2221 agcccgatcc cgatcgctac gagcagcgag aggtacgcac cccccgccgc gacacccgca
     2281 acccccacgt cgctcgcacc cccctgggtt gcatcggggg cggcggtggc gcgttcggcg
     2341 cggcgttcta gccaggcgtc gatgcccgct aagaccaggt aggcgaccag cgctccggcg
     2401 aagacgaggc tggcgccgcc gaacgcggcc ggcgcttcgg cggcgatctc caggccctcg
     2461 agcgtggcgt cgatcgcgag gaaggcgagc aggccgacgg tgaaggcgag cagcgccgcc
     2521 atccactgcg agccgaggcg gcgcaggaac ggcagccaga gcatgccgag actgaccggg
     2581 atcacgccga cgtagatccc cagcagcgcc atcagcccgt agaagtcgag gtcggcgtcc
     2641 ggcgtttccg ccgcgaccgg gatttccgcc gtgatcgtgc cgccgaccgc ggtgaggatg
     2701 aagatgtcgt aggcctcgcc ttcgatccag gggtaggtga tgtcgagcgt ggtcgagccg
     2761 agccggccga tctcgcggcc gccgtcggcg gtgaacggcg cgaagccgtc gttgaccgcg
     2821 acctgggcga tttcgaccgg gtcggggccg tcgttctgca gggtcagctc gatctggccc
     2881 gggtgcagga cggtgctctc gaccgagact tcctcgaccg gcgggccgtt gcggtcgccg
     2941 aggccgggac cgccgagcgc cgcgaaggtg ccgatcgcga ccgcgatcag gagcagcggg
     3001 atcagcccca gcgcccagga gggcaggccg ctcccgaccg gtcgctcggc gcggctcccc
     3061 gcctccatca ggccttcgct ccccagggca ggtcgcagta catcgaggca gcggccgccg
     3121 cggtcagctt gctcgggtcg ccgacgcgga agaacccggt ccagccgagc tcggcgaact
     3181 cggtcacgtg ggcgtggaac atgtagtcgc cgtcgaacgg gaactcgagc tcgaggatcg
     3241 cccgctgcgc ctgcccctgg atgatcgtgt cggtgaactc gatcggcttc agcgaggtgc
     3301 cagttggata gtgatggaag aagttgccgt ggatgtggaa cgagttgacc gggtcgtact
     3361 cgagcacgtt gaccaggtag atccggacca gctcgtcacg cggaaccgca atcggctcgt
     3421 tgatgtagtg gaagccgacc gtgttgaccg cgtagacctc gttttcgccg tcgaagttgg
     3481 tgtcgaagcc gttcatcacc atcaccagct cgtcggcttc cgggcgcccc tccttcgggt
     3541 cgatgatgaa ggcgccgtag aggccgcggg cgatgtgggc ggcgagcggg gcgacgtggc
     3601 agtggtagag gtggacgccg aacggcgagg cgtcgaattc gtaggtgaac gcttcgcccg
     3661 gttcgatctg gccgccgccg cggtcctcgc cgattcccgg catcccgtcc atcagcgccg
     3721 tgtggacgcc gtggaagtgc atcgtgtgcg ggtgctcgga gccgttgatg aacttgatcc
     3781 gcagcagctc cccctcgcgg gcgcgcaggg tcgggccggg gacgcggccg ttgtaggtcc
     3841 aggcttcgta ggagacgccg ggggcgactt cgatctcgcg gtcggcggcg acgatctccc
     3901 attcgcgcag cacccggccg ctgggcaggc ggcgggtctt gccgtaatcg aagtcgcgca
     3961 ggatctcagt cgggttgaag ccgttgcgat cgtggtcgac gacgcggtcg ggagcgaagc
     4021 cggcgtggat gaagccgccg tgctcgtgtc cctgcagcgg cgattcggtc gccgcctcgc
     4081 cgttggtcgc gagcgccgcc ccgctgttgc cgttgaccag cccgttcagc ccgatcgcgg
     4141 ctggcacgct cagcgccgca gcgccgccga gcaggcggcg tcgactgatc ccgttcttgg
     4201 tgctgtctgg agccatcggc cggagatttt gacacatcta aaactgaatt tcgagtcatc
     4261 taaaaaccgg actcgaaaca agacccgcga aagccggcga ttgggccttc gtagaatcgg
     4321 cccgtggcga ctgagaccga gatcgagctt gacgcgcagt accggcagct gcgcgaggag
     4381 tgcgggctgc tcgagcgcag cgagcgcagc gagctgctgg tcagcggcgc cgacgcggcc
     4441 gagtacctac aggggcagct aactaacgac accgaggcgc tggagcccgg cgacggtctc
     4501 tacgcggcgc tgctcgaccg caaggggcac atgcagtccg acatgcgggt gctgcgcccg
     4561 ggcgcgggtc ccgacctctt gctcgacctc gaaacgagcg ccctgcccgc ggtgctcaaa
     4621 cacctgacga tgtactcgat cgggcgcgac gtggcggtcg ccgacgccag cgaggagcgc
     4681 gccctgttct cgctgatcgg cccgcgcagc gtcgagctgg ccgggaccgc ggcgctgccc
     4741 gaggacgcct gcgaggagac cagcgtggcc ggggtcgagg tcgtcgccgc cggcacccgc
     4801 gagggcatcg acctcttctg cgccgccacc gaggccgagc gcctacgcgc ggcgttgctg
     4861 gaagccggcg ccgtcgcggt ctcccccacc gccgccgaga tcctccggat cgagtccggc
     4921 gtgccccgct acggggccga gatgggcggc gagacgatgc cggccgaggc ggggatcgtc
     4981 gagcgcgcgg tcagcttcac caagggctgc tacatcggcc aggagacggt cgcccggctg
     5041 cactacaagg gcaagccgaa ccgccacctc cgggggttga ggctcaccga tccggtcacc
     5101 cagggtgacg agatacggct aggcgagcgc gtggtgggcg agatcggctc cgccgccgtg
     5161 tcgccggcgt tcgggccgat cgcgctggcc gtggtgcgtc gcgaggccga gcccggcacg
     5221 gtcgtcagcg tggggccgtc cgacgtggac ggaag
//
LOCUS       DASRIG010000522         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2570955, whole genome shotgun sequence.
ACCESSION   DASRIG010000522 DASRIG010000000
VERSION     DASRIG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570955"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /gene="mnmA"
                     /locus_tag="VFB52_13990"
     CDS             <1..1369
                     /gene="mnmA"
                     /locus_tag="VFB52_13990"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZO07496.1"
                     /translation="AAGGAACGDLSRISFQVEDGRLTKVTFDAEGCGATEAATAAVAE
                     TIDGGTVLEAALLSIDDAEALLGGLTPAKRHAAQLATDALHRGLSAAAASPLPLATDG
                     GRVAVAMSGGVDSAVAALLERERGAEVIGITVKLWADPETDGTKACCSPEAVLGARAL
                     AHQLGIPHFTLDLEAEFRQRVVGLFLSGYAEGKTPNPCILCNGEVRIAAMVDLAERLG
                     ADRLLTGHYARIVEDADGPLLAAGADPAKDQSYMLAALPPNLLARLGFPLTELTKPEV
                     REIAARHQLAVAKKPESQDLCFLAGQGKRGFLRRHGGLEDREGAVLDSSGRTLGRHRG
                     HHNFTVGQRRGTGVSINEPAYVLATDAVANTVTIGTRAELVTRHVRVRDAILHRDGAR
                     VDAVRLRYRSRPLPASIGAPAAGRHEALDLQLGEEFAGAAPGQTAVLLEGETIVGHGT
                     IVGNV"
     gene            1394..4033
                     /gene="alaS"
                     /locus_tag="VFB52_13995"
     CDS             1394..4033
                     /gene="alaS"
                     /locus_tag="VFB52_13995"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZO07497.1"
                     /translation="MKAQEIRDTYLSFFEERGHKVVPSASLVPSAHDPSVLLTTAGMQ
                     PFKPYFLGREKPPASRLADVQKCFRTTDIEEVGNTARHLTFFEMLGNWSFGDYFKAES
                     IPWGWELSTKGFGMDPEKIWVTVFGGDEELGLGPDEEAIAHWGAIGVPDERIVRLGRK
                     DNFWQGGPTGPCGPCSELYLDRGLDFGAEDDLPGSDSDRFLEFWNHVFMSYDLHEDGS
                     LTELPSKNIDTGMGLDRMAAILQDVPSVYEVDHIRPLVDLAEELSGHGYEEGERVTRA
                     MRIVADHARGATFLLADGVVPSNEDRGYILRRIMRRAIQQGNSLGLESPWLGKFAERT
                     IELMGETYPELLAERETIARWVGDEEESFGRTLERGTELLERLVAEAKESETSWVDAA
                     DAFKLHDTYGFPYDLTKELLAEEGLAVDDSGFEELMEEQRQRARMGSATAHGSEDSHD
                     RVLAFADESEKTEFVGYETLRTTTGLAAAAVQDEDRALVKLEQSPFYAEGGGQVSDSG
                     VVRWNGSEASVRDVYRVGDDQVVELTSGGIEIEPGVSVEAEVDHEARYATMRNHTATH
                     LLHAALRERLGTHVRQAGSAVRPDKLRFDFTHGQQLSLDELRDVEDRVNEWVKASRPV
                     RWLEMERAEAERLGAMALFGEKYGEWVRMVEVDGVSRELCGGTHVANTAEVGIFKVTS
                     EGSSAANVRRVEAITGPEAIDYFRERESQLREAGELLGNPQDPVGGARRAAEKLKEAG
                     AGAQKAAAAALGEEADQLAGAGVELAGGKVTLVQATSASATDAKQLLDLANRVQSKLG
                     GSGVVVTLGGDFGEKAGLIALASKDAVAAGVSAGALVKEGAPAIGGGGGGRDDMAQAG
                     GRDPSKLDEALSAMRTAIERELG"
     gene            4039..4440
                     /gene="ruvX"
                     /locus_tag="VFB52_14000"
     CDS             4039..4440
                     /gene="ruvX"
                     /locus_tag="VFB52_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZO07498.1"
                     /translation="MWVLALDHGTVRVGCALSDPSGTLATPLPVIEPPEPRAVAELVA
                     EHGVERVVVGLPVHLSGEEGSQAALARTFCGELEAMLSIPVETYDERLTSRMADASRR
                     AGASAARDSLAAAHLLEAYLLTMAGREGSDA"
     gene            4437..>6006
                     /gene="mltG"
                     /locus_tag="VFB52_14005"
     CDS             4437..>6006
                     /gene="mltG"
                     /locus_tag="VFB52_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZO07499.1"
                     /translation="MSEDWRDDPFVDSDDPAARERAERRAEREAKRAEREGKRKQKEA
                     AKQPPPEPEPPAEPPKPPRTPEQEFWDEDPEPAPEAKAPAAEPKAEESKPKEPEQPEP
                     KQPRKAEPVAAAATAAAAGAAADPVPTPAPEPAVEQAWEKAPPSTPDHDIWGEEGDPD
                     KARAARRRDGGDGNGGGRGSKAASGWRRYHPLKIAGLIVVFLVLWFVYALFQPFHSGS
                     SDEQVRVKVPKGASVSEVGDLLLDKGVIDDPILPVSGSTLFQIRVTLAGKRSSLYAGT
                     FTLNKGMSYGDAIDALSTPPQKRTTTVTIPEGYTRSQAAQLVEEDGVPGSYTKKTVKS
                     KYLDPADYGGKNAESLEGFLFPNTFELKPKAPVGDLVELQLKDFKKQIKKVDMGYAKS
                     KNLTVFDVLIIASMIEDEAGVPKQRKQVAAVIYNRLKDGMPLGIDATIRFHTGNYTKP
                     LTESELNEDSPYNTRENTGLPPGPINSPGLAAIQAAAHPAKSDYLFYVNKPNTCNELA
                     FSSNEDEFEEDVAAY"
BASE COUNT         1017 a         2151 c         2089 g          749 t
ORIGIN      
        1 cgccgcgggt ggtgccgcct gcggcgatct ctcccggatt tcctttcagg tggaggacgg
       61 gcggctcacc aaggtcacct tcgacgcaga gggctgcggc gctaccgaag cggccaccgc
      121 ggccgtggcc gagaccatcg acggcggcac cgtgctcgag gcagcgcttc tctcgatcga
      181 cgacgccgaa gcgctgctcg gcggcctcac gccggcgaag cgccacgccg cccagctcgc
      241 caccgacgct ctccaccgcg gcctcagcgc cgccgcggcg tcaccgctcc cgcttgcgac
      301 cgacggcgga cgtgtcgccg tcgccatgtc cggcggcgtc gactccgccg tcgctgcgct
      361 gctcgagcgc gagcgcgggg ccgaggtcat cggcatcacg gtgaagctct gggccgatcc
      421 cgagaccgac gggaccaagg cctgctgctc gccggaggcg gtgctggggg cgcgggcgct
      481 ggcgcaccag ctcgggatcc ctcacttcac gctcgacctc gaggccgaat tccgccagcg
      541 ggtcgtcggg ctcttcctct ccggctacgc cgagggcaag acgccgaacc cctgcatcct
      601 ctgcaacggc gaggtgcgga tcgcggcgat ggtcgacctc gccgagcggc tcggtgccga
      661 tcggctgctg accggccact acgcgcggat cgtcgaggac gcggacggac cgctgctcgc
      721 cgccggcgcc gatccggcca aggaccagag ctacatgctc gcggcgctgc cgccgaacct
      781 gctcgcgcgg ctcggcttcc cgctgaccga gctgaccaag cccgaggtgc gcgagatcgc
      841 cgcccgccat cagctcgcgg tcgcgaagaa gccggagtcc caggacctct gcttcctcgc
      901 cggccagggc aagcgcggtt tcctgcgccg ccacggcggc ctcgaggacc gtgagggcgc
      961 cgtgctcgac agctccggcc gcacgctcgg ccgccaccgc ggccaccaca acttcaccgt
     1021 cggccagcgg cgcggcaccg gcgtctcgat caacgagccc gcctacgtgc tcgccaccga
     1081 cgccgtcgcc aacacggtca cgatcggcac ccgcgccgag ctggtcaccc ggcacgtgcg
     1141 cgtccgcgac gcgatcctcc accgcgacgg cgcccgggtc gatgccgtcc gcttgcgcta
     1201 ccgctcgcgc ccgctcccgg cgagcatcgg cgcccccgcc gcggggcgcc acgaggcgct
     1261 cgatctgcag ctcggcgagg agttcgcggg ggccgctccg gggcagaccg cggtgctgct
     1321 cgaaggcgag acgatcgtcg gtcacggcac gatcgtcggc aacgtctgag cagagtattt
     1381 aggctccctc acaatgaagg cccaggagat ccgcgacacc tacctctcgt tcttcgagga
     1441 gcgcggccac aaagtggtcc cgtcggcgtc cctggtgccc tccgcgcacg acccctcggt
     1501 gctgctcacg accgccggga tgcagccgtt caagccctac ttcctgggcc gtgaaaagcc
     1561 gccggcatcg cgcctcgccg acgtgcagaa atgcttccgc acgaccgaca tcgaggaggt
     1621 cggcaacacc gcccgccacc tcaccttctt cgagatgctc ggcaactgga gcttcggcga
     1681 ctacttcaag gcggagtcga tcccctgggg ctgggagctc tcgaccaagg gcttcgggat
     1741 ggacccggag aagatctggg tgaccgtgtt cggcggcgac gaggagctcg gcctcggccc
     1801 cgacgaggag gcgatcgcac actggggcgc aataggggtg ccggacgagc ggatcgtgcg
     1861 gcttgggcgc aaggacaact tctggcaggg cgggccgacc ggtccctgcg gcccgtgctc
     1921 ggagctctac ctcgaccgtg gcctcgactt cggcgccgag gacgacctcc cgggcagcga
     1981 ctctgaccgc ttcctcgagt tctggaacca cgtcttcatg agctacgacc tgcacgagga
     2041 cggctcgctg accgagttgc cgagcaagaa catcgacacc ggcatgggcc tcgaccggat
     2101 ggcggcgatc ctccaggacg tgccctcggt ctacgaggtc gaccacatcc gcccgctcgt
     2161 cgatctcgcc gaggaactct ccggccacgg ctacgaggag ggcgaacggg tcacccgggc
     2221 gatgcggatc gtcgccgacc acgctcgcgg cgccaccttc ctgctcgccg acggcgtcgt
     2281 cccctcgaac gaggaccgcg gctacatcct gcgccggatc atgcgccgcg cgatccagca
     2341 gggcaactcg ctcgggctcg agtcgccctg gctcggcaag ttcgccgagc gcacgatcga
     2401 gctcatgggg gagacctacc ccgagctgct ggccgagcgg gagacgatcg cgcgctgggt
     2461 cggcgacgag gaggagagct tcggccgcac cctggagcgc ggcaccgagc tgctcgagcg
     2521 cctggtcgcc gaggcgaagg agtccgagac ctcttgggtc gacgccgccg acgcgttcaa
     2581 actgcacgac acctacggct tcccctacga cctgaccaaa gagctgctcg ccgaggaggg
     2641 gctggcggtc gacgactccg gcttcgagga gctgatggag gagcagcgcc agcgcgcccg
     2701 catgggctcc gccaccgcgc acggctccga ggacagccac gaccgcgtgc tcgcctttgc
     2761 cgacgaatcg gaaaagaccg agttcgtcgg ctacgagacc ctgcggacca cgaccggtct
     2821 ggcggccgcc gcggtccagg acgaggaccg cgccctggtc aagctcgagc agagtccctt
     2881 ctacgccgag ggcggcggcc aggtctccga ctccggcgtc gtccgctgga acggcagcga
     2941 ggccagcgtc cgcgacgtct accgggtcgg cgacgaccag gtggtcgaac tcacctccgg
     3001 cgggatcgag atcgagccgg gcgtctcggt cgaggccgaa gttgaccacg aggcccgcta
     3061 cgcgacgatg cgcaaccaca ccgcgacgca cctcctgcac gcggcgctgc gcgagcggct
     3121 cggcacccac gtgcgccagg ccggctccgc cgttcgtccc gacaagctgc gcttcgactt
     3181 cacccacggc cagcagctca gcctcgacga gctgcgcgac gtcgaggacc gggtcaacga
     3241 gtgggtgaag gcgagccgcc cggtgcgctg gctggaaatg gagcgggcgg aggccgagcg
     3301 gctcggcgcg atggccctgt tcggcgagaa atacggcgag tgggtacgga tggtcgaagt
     3361 cgacggcgtc tcccgtgagc tctgcggcgg cacccacgtc gccaacacgg ccgaggtcgg
     3421 gatcttcaaa gtcacctcgg aggggtcgag cgccgccaac gtgcgccggg tcgaggcgat
     3481 cacgggaccc gaggcgatcg actacttccg cgagcgcgag tcgcagctgc gcgaggccgg
     3541 cgagctgctc ggcaacccgc aggacccggt cggcggagcc cgccgcgcgg ccgagaagct
     3601 gaaagaggcc ggggccgggg cgcagaaggc ggccgcggcg gcgctcggcg aggaggccga
     3661 ccagctggcc ggcgccgggg tcgagctggc cggtggcaag gtcaccctcg tgcaggccac
     3721 ttcggcaagc gccaccgacg ccaagcagct gctcgacctc gccaaccggg tgcagtcgaa
     3781 gctcggcggc agtggggtcg tggtcacgct cggcggcgac ttcggcgaga aggccggact
     3841 gatcgcgctc gcctcgaagg acgcggtcgc ggccggcgtc tccgccggcg ccctggtcaa
     3901 agagggtgcc ccggcgatcg gcggcggcgg cggtggccgc gacgacatgg cccaggccgg
     3961 cggccgggat ccttccaagc tcgacgaggc gctctccgcg atgcggacgg cgatcgagcg
     4021 ggagctcggc tgaggctgat gtgggtgctg gcgctggacc acgggaccgt tcgcgtcggc
     4081 tgcgccctct cggacccgag cggcaccctg gcgacgccgc tgccggtgat cgagccgccg
     4141 gagccgcgcg cggtcgccga gctggtcgcc gagcatgggg tcgaacgcgt cgtcgtcggc
     4201 ctgccggttc acctcagcgg tgaggagggc agccaggcgg cgctggcacg gacgttctgc
     4261 ggagaactgg aggcgatgct ctcgatcccg gtcgagacct acgacgagcg cttgaccagc
     4321 cggatggcgg acgcgagccg ccgcgccggc gccagcgccg cccgcgactc gctcgcggcg
     4381 gcgcacctgc tggaggcata tctattgacg atggcgggaa gagaggggag cgacgcatga
     4441 gcgaggactg gcgcgacgac ccgttcgtgg attccgacga ccccgccgcc cgcgagcggg
     4501 cggaacggcg agccgaacgc gaagccaagc gcgccgagcg cgaaggcaag cgcaagcaga
     4561 aggaggcggc gaagcagccg ccgccggagc ccgagccgcc cgccgagccg ccgaaaccgc
     4621 cgcgcacgcc cgagcaggag ttctgggacg aagaccccga gccggccccg gaagcgaaag
     4681 ctccggccgc cgagccgaaa gctgaggagt cgaagcccaa agagccggag cagcccgagc
     4741 ccaaacagcc tagaaaggca gagccggtcg ccgccgcggc gaccgccgcc gctgcgggtg
     4801 cagccgccga cccggtcccg accccggcgc cggaaccggc ggtcgagcaa gcctgggaga
     4861 aagcgccacc ctcgaccccg gatcacgaca tctggggcga ggagggcgac ccggacaagg
     4921 ctcgcgccgc acgccgccgc gacggcggcg atggcaacgg gggaggccgc ggcagcaaag
     4981 ccgcgagcgg ctggcgccgc taccacccgc tgaaaatcgc cggcctgatc gtcgtcttcc
     5041 tcgttctctg gttcgtctac gcgctgttcc agccgttcca cagcggcagc tccgacgagc
     5101 aggtccgggt caaagtcccg aagggggcga gcgtcagcga agtcggcgac ctgctgctcg
     5161 acaaaggcgt gatcgacgac ccgatcctgc cggtcagcgg ctcgaccctg ttccagatcc
     5221 gggtcaccct ggcgggcaag cgctcctcgc tgtacgcggg cacgttcacc ctgaacaagg
     5281 ggatgagcta cggcgacgcg atcgacgcgc tctcgacccc gccgcagaaa cggacgacca
     5341 cggtgacgat cccggagggc tacacgcgct cgcaggccgc gcagttagtc gaggaagacg
     5401 gggtgccagg cagctacacg aagaagaccg tgaaatcgaa gtacctcgac cccgccgact
     5461 acggcggcaa gaacgccgaa agcctcgagg gcttcctctt cccgaacacc ttcgaactga
     5521 agccgaaagc gccggtcggc gacctggtcg agctgcagct caaggacttc aagaaacaga
     5581 tcaaaaaagt cgacatgggc tacgcgaagt cgaaaaacct gacggtcttc gacgtcctga
     5641 tcatcgcctc gatgatcgag gacgaggccg gggtgccaaa gcagcgcaag caggtcgccg
     5701 cggtgatcta caaccggctc aaagacggga tgccgctggg gatcgacgcg acgatccgct
     5761 tccacaccgg caactacacg aagccgctga ccgaatcgga actgaacgaa gactcgccgt
     5821 acaacacgcg cgagaacacc ggcctgccgc cgggcccgat caacagcccc gggctggccg
     5881 cgatccaggc agccgcccac ccggccaaat ccgactacct cttctacgtg aataagccga
     5941 acacgtgcaa cgaactcgcc ttctcctcga acgaagacga attcgaagaa gacgtcgccg
     6001 cgtaca
//
LOCUS       DASRIG010000523         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3113214, whole genome shotgun sequence.
ACCESSION   DASRIG010000523 DASRIG010000000
VERSION     DASRIG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3113214"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFB52_14010"
     CDS             <1..666
                     /locus_tag="VFB52_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZO07500.1"
                     /translation="PVLELRDVRKSYPGGVEALRGVSLAIGAGELTAIVGPSGSGKST
                     LLHMMGTLDRPTSGSVAVEGREVSAASDDELAGVRATRIGFVFQQFFLLEAAPVIDNV
                     ADGLLYAGVPAGERRRRASEALARVGLSHRERHSASKLSGGERQRVAVARALVGEPAI
                     ILADEPTGNLDSRTGEQLMELLRELSAAGATLVIITHDDEIARSLPRRIAVRDGRVET
                     DDG"
     gene            659..1852
                     /locus_tag="VFB52_14015"
     CDS             659..1852
                     /locus_tag="VFB52_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07501.1"
                     /translation="MASRLLPRDMLRLATIGLRTRKLRAALSVLGISIGIASLVAVLA
                     ISETSKADLLATIEKLGTNLLTVGPGESLFGEETELPGAAAGRVRMLPEVESASSVTQ
                     VGGVTVRRSSYVPEEVTGGIAVRAAEDSLLTTLDGELRDGRFLDRASERYPAIVLGAK
                     TAQRLGIERVEGVTQVYLSGRLFTVVGILEPLRLAPEIDSSALIGYPIAKQLFGTERS
                     ASQIYVRTLDGEADVNRAAALLPSAADPWDPDEVAVSRPSDALEAQAAAEGAFTSLFL
                     GLGAVALLVGGIGIANVMIISVLERRSEVGLRRALGARRRHIATQFLSESFVLGILGG
                     AAGVLLGAGATAAYAAFEGLELLVPPLAIGGGLLAACLLGGLAGLYPALRAARLTPTE
                     ALRSV"
     gene            1988..2521
                     /locus_tag="VFB52_14020"
     CDS             1988..2521
                     /locus_tag="VFB52_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZO07502.1"
                     /translation="MADKSFADALNEQIANEFAASQQYVGAAVYYDAETLPRLAAFFY
                     RQALEEREHAMMMIQYLLDVGEGVHVPDIKSAETTYADGSEPVKMALEQEKRVSDEIF
                     ALFELARDLKDYRAEQFLQWFVKEQVEEVALMGDLLNVVERSMDNLLLAEEYIARESL
                     GEEGSDPTAPPAAGEGH"
     gene            complement(2525..3376)
                     /locus_tag="VFB52_14025"
     CDS             complement(2525..3376)
                     /locus_tag="VFB52_14025"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HZO07503.1"
                     /translation="MGLSGGGSAGNGSADSAPATAVRRILALGGHDFTSRPPDRAVCE
                     LLLRLAGERGAERPKICILPTASGDTSEQIGRFYAAFGERSCEPSDLSLFRLGRKPMA
                     LRDHLLEQDLIYVGGGSLVNLLAVWEAHDIASILSLAWRRGIVLAGQSAGAMCWFEAG
                     ITKSSGKPLAAAGLGLLSGSLCVHYNNEPERRAAYLEAVADGMPGGYGLDDYAGLIWE
                     GEGTPTAVTARRGARAYRVSDRDGTVSESPLPARFLPAPAPAALREDIAEFRRIKTMR
                     KRAGWLG"
     gene            complement(3376..4143)
                     /locus_tag="VFB52_14030"
     CDS             complement(3376..4143)
                     /locus_tag="VFB52_14030"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HZO07504.1"
                     /translation="MKTVPKQIVAFGGGGFSMESGNPLLDDYVLGLTKAERPRVCFLP
                     SASGDADHYIVRFYRAFSAHRCEASHISLFRREQGPEDLRRHLLSQDLIYVGGGSVIS
                     LLGVWRAHGIDVILREAWEAGVILCGLSAGSLCWFAEAVTGFHGAPRRLEGIGLLPFS
                     NCVHYEPKSSRRESYHRFLREGMRGGYAAEDGAALHFTDQELSRVVASRPHARGYRLD
                     VSGQRVVEMRIATTYLGERDAIPAAPLPEALSTAVAA"
     gene            complement(4254..4751)
                     /locus_tag="VFB52_14035"
     CDS             complement(4254..4751)
                     /locus_tag="VFB52_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO07505.1"
                     /translation="MSTPIAHRTGKDDGWEWGEAHRVCLRLAYRYASNPSEAEDIAQD
                     ALLRAWRRRATLREPERRKQWLATIVRNEAFRQHARVRPIPTDLVEGEAVEDARVVAT
                     VELADLHAALRQLSEKDRDLLEMRYQDDLTQGAIARKLGIPEGTVKVRLHRARNKLRR
                     VYVPR"
     gene            4845..5633
                     /locus_tag="VFB52_14040"
     CDS             4845..5633
                     /locus_tag="VFB52_14040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07506.1"
                     /translation="MAGEGRPRWAEIAYEAIAPVYDEFTHENDYETWFGLLLPELERH
                     GLRTGRLLDVGCGSGKSFAPMLARGWSIVGCDVSAAMLKRGREKVGDSVRLEVADMRE
                     LPTYGEFELVWALNDPVNYMLDEQELDLALDGMRRNLSPDGLLMFDANTILTYRGFFA
                     EQIEFERDGWRMIWRGRSSAEIEPGAVSEAEFEAVPLEEGVGEPITPELHRERHFPLE
                     QILAALERSGLECLDVFGYGVDEAMHQPLDEDEHYKAIYIARAV"
     gene            complement(5630..>5906)
                     /locus_tag="VFB52_14045"
     CDS             complement(5630..>5906)
                     /locus_tag="VFB52_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07507.1"
                     /translation="RRFEPDGPALSLPPERLAEAKADGAGGGWPEVGAAIMDGVAVTL
                     KLRTTDGEKLKLMMMRGTGPLAGLGGGEDQRTGVEALAAWFEAAARG"
BASE COUNT          880 a         2069 c         2073 g          884 t
ORIGIN      
        1 ccggtgctcg agctgcgcga cgtgcgcaag tcctaccccg gcggggtgga ggcgctgcgc
       61 ggggtctcgc tcgcgatcgg ggcaggagag ctgaccgcga tcgtcggccc ctcgggctcg
      121 ggcaagtcga ccctgctgca catgatgggc acgctcgacc ggccgaccag cggcagcgtc
      181 gcggtcgagg gccgcgaagt ctccgccgcc tccgacgacg agctggccgg ggtgcgggcg
      241 acccggatcg ggttcgtctt ccagcagttc ttcctgctcg aggccgcccc ggtgatcgac
      301 aacgtcgccg acgggctgct ctacgccggc gtgccggcgg gcgaacggcg tcggcgggca
      361 tccgaggcgc tcgcccgggt cggcctctcc caccgcgagc gccacagcgc ctccaagctc
      421 tccggcggcg agcgccagcg ggtagcggtg gcgcgggcgc tggtcgggga gcccgcgatc
      481 atcctcgccg acgagccgac cggcaacctc gacagccgca ccggcgagca gctgatggag
      541 ctcctgcgcg agctgagcgc ggccggggcg acgctggtga tcatcaccca cgacgacgag
      601 atcgcgcgct cgctgccgcg gcggatcgcg gtccgcgacg gacgggtcga gaccgatgat
      661 ggctagccgc ctcctgcccc gcgacatgct gcgcctggcg acgatcgggc tgcgcacgcg
      721 caagctccgc gccgccctct cggtgctcgg gatctcgatc gggatcgcct cgctggtcgc
      781 cgtcctggcg atctcggaaa ccagcaaggc ggacctcctg gcgacgatcg aaaagctggg
      841 caccaacctg ctcacggtcg gccccggcga atcgctcttc ggcgaagaaa ccgagctgcc
      901 cggggcggcg gcgggtcgcg tgcggatgct gcccgaggtc gaatcggcga gctctgtgac
      961 ccaggtcggc ggcgtcaccg tgcggcgcag ctcctatgtg cccgaagagg tgaccggcgg
     1021 gatcgccgtg cgcgccgccg aggactcgct gctgaccacg ctcgacggcg agctgcgcga
     1081 cggccgcttc ctcgaccgcg cgagcgagcg ctatccggcg atcgtgctcg gggcgaagac
     1141 cgcccagcgg ctcgggatcg aacgggtcga aggcgtcacc caggtctacc tctccggtcg
     1201 tctgttcacc gtcgtcggga tcctcgaacc gctgcgcctg gcaccggaga tcgacagctc
     1261 ggccctgatc ggctacccga tcgccaagca gctgttcggg accgagcgca gcgcctcgca
     1321 gatctacgtg cgcacgctcg acggcgaggc cgacgtcaac cgcgccgccg ccctgctgcc
     1381 gagcgccgcc gacccctggg accccgacga ggtcgcggtc agccggcctt ccgatgcgct
     1441 cgaggcccag gccgccgccg agggcgcctt cacctcgctc ttcctcgggc tcggtgccgt
     1501 tgccctgctg gtcggcggga tcgggatcgc caacgtgatg atcatctcgg tcctggagcg
     1561 tcgctcagag gtcgggttga gaagagcgct cggagcccgc cgtagacaca tcgccaccca
     1621 gttcctctcc gagtccttcg tgctcgggat cctcggcggc gccgccgggg tgctgctcgg
     1681 cgccggtgcc accgccgcct acgcagcctt cgaagggctc gaactgctgg tcccgccgtt
     1741 ggcgatcggg ggcggcctgc tcgccgcgtg tttgctcggt ggcctcgccg ggctctaccc
     1801 ggcgctgcgc gccgctcggc tcacccccac ggaagcgctg cgctctgtgt gaggtcgcat
     1861 ttgttgccgg tatgcggcaa gaaatgcgac ctcacgggtt tggctggcct aagcgtttct
     1921 tcggccaacc aaatccggcg tttcggctac gttgggtttc ccagccccgc cgacaaggag
     1981 cagaccgatg gcagacaagt ccttcgccga tgcactgaac gagcagatcg ccaacgagtt
     2041 cgccgcctca cagcagtacg tgggcgccgc ggtctactac gacgccgaga cccttccgcg
     2101 gctcgctgcc ttcttctacc gacaggcgct ggaggagcgc gagcacgcga tgatgatgat
     2161 ccagtacctg ctcgacgtcg gcgagggcgt gcacgtcccc gacatcaagt cggccgagac
     2221 cacctacgcc gacggctccg agccggtgaa gatggcgctc gagcaggaga agcgggtcag
     2281 cgacgagatc ttcgccctgt tcgagctcgc ccgcgacctc aaggactacc gggccgagca
     2341 gttcctgcag tggttcgtca aggagcaggt cgaggaggtc gcgctgatgg gcgacctgct
     2401 gaacgtggtc gagcgctcga tggacaacct gctgctggcc gaggagtaca tcgcccgtga
     2461 gagcctcggc gaggagggct cggacccgac cgcgccgccc gccgccggcg agggccactg
     2521 acgctcagcc cagccagccc gcccgcttgc gcatcgtttt gatccgccgg aactcggcga
     2581 tgtcctcgcg cagcgcggcg ggagcggggg ccgggaggaa gcgggccggg agcggggact
     2641 cggagaccgt gccgtcgcgg tcgctgaccc ggtaggcgcg ggcgccgcga cgggcggtga
     2701 ccgcggtcgg cgtgccttcg ccttcccaga tcaggccggc gtagtcgtcg aggccgtagc
     2761 cgcccggcat cccgtcggcg accgcctcca gataggcggc gcggcgctcg ggttcgttgt
     2821 tgtagtggac gcagaggctg ccggagagca ggccgagccc ggccgccgcc agcggcttgc
     2881 ccgaggactt ggtgatcccg gcctcgaacc agcacatcgc gccggcgctc tggccggcga
     2941 gcacgatccc gcgccgccag gcgaggctga ggatcgaggc gatgtcgtgc gcctcccaga
     3001 ccgccagcag gttgaccagg ctgccgccgc cgacgtagat caggtcctgc tcgagcaggt
     3061 ggtcgcgcag cgccatcggt ttgcgcccga gccggaacag cgagaggtcg gagggctcgc
     3121 aggagcgctc accgaaggcg gcgtagaagc ggccgatctg ctcggaagtg tcaccgctgg
     3181 cggtcggcag gatgcagatc ttcggccgct cggcgccgcg ctcgccggcc aggcgcagga
     3241 gcagctcgca gacggcgcgg tcgggcgggc gcgaggtgaa gtcgtggccg cccagcgcga
     3301 ggatccggcg gaccgcggta gcgggcgccg aatcggccga gccattgccg gcgctgccgc
     3361 cccctgacaa gcccatcagg ccgcgaccgc ggtcgacagc gcctcgggca ggggcgcggc
     3421 cgggatggcg tcgcgctcgc cgaggtaggt ggtggcgatc cgcatctcca ccacccgctg
     3481 tccggagacg tcaagccggt agccgcgcgc gtgcggccgc gaagcgacaa ccctgctcag
     3541 ttcctggtcc gtgaagtgca gagcggcgcc gtcttcagct gcgtagccac cgcgcatgcc
     3601 ctcgcgcagg aagcggtggt aggactcgcg ccgactcgat ttcggctcgt agtggacgca
     3661 gttgctgaac ggcagcaggc cgattccctc cagccgccgc ggggcgccgt ggaagccggt
     3721 gaccgcctcg gcgaaccagc agagcgagcc ggccgagagc ccgcagagga tcacgccggc
     3781 ttcccacgct tcgcgcagga tcacgtcgat cccgtgcgcg cgccagaccc cgagcaggct
     3841 gatcacgctg ccgccgccga cgtagatcag gtcctgggag agcaggtggc ggcgcaggtc
     3901 ctcgggcccc tgctcgcggc ggaacaggga gatgtggctg gcctcgcagc ggtgcgcgga
     3961 aaaagcgcgg tagaagcgca cgatgtagtg gtcggcgtcg ccgctggccg aaggcaggaa
     4021 gcagacccgg ggacgctcgg ccttggtcag gccgagcacg tagtcgtcga gtagcggatt
     4081 cccggactcc atcgagaagc cgccccctcc gaacgccacg atctgcttcg ggaccgtttt
     4141 catgtaccta atagtcggtc acgaagcccc ctcaggcctt gtacagcggt ctgaagttcc
     4201 cctccgatga atcgcccgtg tgtacaggcg cataatcggt tcctaaacgg ccgtcagcgg
     4261 ggtacgtaga cgcggcgcag cttgttgcgg gcacggtgca ggcgcacctt caccgtgcct
     4321 tccgggatcc ccagtttgcg cgcgatcgcg ccctgggtga ggtcgtcctg gtagcgcatc
     4381 tccagcaggt cccggtcttt ttcgctgagc tgccgcagag ccgcgtgcag gtcggcgagc
     4441 tcgaccgtgg ccaccacccg cgcgtcctcg actgcctccc cctcgaccaa gtcggtcggg
     4501 atcggccgaa cccgggcgtg ctggcggaac gcctcgttgc ggacgatcgt cgccagccac
     4561 tgcttgcgac gctcgggctc acgcagagtc gcgcgccgac gccaagcgcg gagcagagca
     4621 tcttgggcaa tgtcttcggc ttcgctcgga ttgctcgcgt accgatacgc cagcctcaga
     4681 caaaccctgt gtgcctctcc ccactcccag ccatcgtctt tccccgttcg atgtgcgatt
     4741 ggagtgctga taaaacgaac ctacatctct cataaccgcc taggggcgat aaaccgctgt
     4801 aaccatttcc agtaaacccc ggtctttgcg tcgtgcaggt gaaaatggca ggtgagggac
     4861 gacctcgttg ggccgagatc gcttacgagg cgatcgctcc cgtctacgac gagttcaccc
     4921 acgaaaacga ctacgagacg tggttcgggc tgctcttgcc cgagctcgaa cggcacggtc
     4981 tgcggaccgg ccggctgctc gatgtcggct gcggctcggg caagtcgttc gcgccgatgc
     5041 tggcgcgcgg ctggagcatc gtcggctgcg acgtctcggc ggcgatgctg aagcgcgggc
     5101 gcgagaaggt aggtgactcc gtgcggctgg aggtcgccga catgcgcgag ctgccgacct
     5161 acggcgagtt cgagctggtc tgggcgctca acgacccggt caactacatg ctcgacgagc
     5221 aggagctcga cctcgcgctc gacgggatgc gccgcaacct cagtcccgac gggctcctga
     5281 tgttcgacgc caacacgatc ctcacctacc gcggcttctt cgccgagcag atcgagttcg
     5341 agcgcgacgg ttggcggatg atctggcgag ggcgctcgag cgccgagatc gagcccggcg
     5401 cggtcagcga ggcggagttc gaagcggtgc cgctggagga gggggtcggc gagccgatca
     5461 cgcccgagct gcaccgcgag cgccacttcc cgctcgagca gatcctcgcc gcgctggagc
     5521 gcagcggcct cgagtgcctc gacgtgttcg gctacggcgt cgatgaggcg atgcaccagc
     5581 cgctcgacga agacgagcac tacaaggcga tctacatcgc ccgcgcggtc tagccgcgcg
     5641 ccgcggcctc gaaccaggcg gcgagcgcct cgaccccggt ccgctggtcc tcgccaccgc
     5701 cgagcccggc gagcgggccg gtcccgcgca tcatcatcag cttcagcttc tcgccgtcgg
     5761 tcgtgcgcag cttcagggtg acggcgacac cgtccatgat cgcggcgccg acttccggcc
     5821 agccgccccc ggcaccatcg gccttggcct ccgccaaccg ctcgggcggc agcgagagcg
     5881 ccggcccgtc gggctcgaac ctgcgg
//
LOCUS       DASRIG010000524         5014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2312189, whole genome shotgun sequence.
ACCESSION   DASRIG010000524 DASRIG010000000
VERSION     DASRIG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312189"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFB52_14050"
     CDS             complement(<1..311)
                     /locus_tag="VFB52_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07508.1"
                     /translation="MRRAGSWPLLWAIPVIALALAAPAAASSSAPFGHACVAQDGVRF
                     CPTQTPAERVPSFDGVPLDVDVTLPATGNGPWPTIVMMHGFGGSKTNFETTSPAGPAP
                     E"
     gene            390..1472
                     /locus_tag="VFB52_14055"
     CDS             390..1472
                     /locus_tag="VFB52_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07509.1"
                     /translation="MTRLRAHITALMSTAAIAAVAILVLAVTDSAQAAPLEPPGKKIY
                     FGVSDTGDPADFGAFSKASGQHPAVIQSFRTWGADFPESIRRWQTARARPMLHISTAD
                     NNDGHELITPAQIAAGYGDEYLLRLNKLFWNKRLAAYIRPLGEPNRCLNVYAAYDCEG
                     NFRDAAHSPRNYRRAFRRIYILVHGGGKARKINARLHEAGLPPLTVDVPGLPAAPIAI
                     VWSPLPAGSPTVPQNRPRHFYPGNKWTDWVGTDFYAAYPEWKALNGVYKRFKGKPFAL
                     TEWGVEHGDDPSFVRGLFNWVKSHPRADMLVYYQDFGSSSSYRIQNWPSSLSVMRNQL
                     RSRLFPPFAKGFPRLPPPPPGGVGAG"
     gene            complement(1469..3019)
                     /locus_tag="VFB52_14060"
     CDS             complement(1469..3019)
                     /locus_tag="VFB52_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZO07510.1"
                     /translation="MAEGGPKARRIRDTFVLLVRHPRRFWEEKRTLALVGIAVLVLAI
                     FAVVVAYQELKRPADVHNEDVAFEATKPKAPPPVAKTVNWPLFGLNPARTRYLPAKGV
                     APPFRKLWRYTDRPLLEFPPIFVGGTLYAVNNSGFAFALDAKNGRTLWKRRIGTLNAS
                     SPAYSKHRLYIVNLVPGHILKLDAKTGRTIWKRDLPDRAESSPLVIGNTVYFGCENGR
                     LYALSTVNGNERWSTELGGDVKAAPAYFGGRLFVGDYGGHMNAVDAESGKLIWQSGSL
                     GPGFGGSGAFYSTPAVAFGRVYAGNNDGRVYSYGLKDGTLAWTYSTGGYVYSGPTVAS
                     TRHSPPSIFIGSFDGNIYSLDAKDGSVRWSRDAGGQVIGSLSAVGDIVYAAEFTNKTT
                     SGYMMRSGRRVFEYPKGTYTPVISDGRRIYLTGYSSITALQPYNPKRLRAVRLKLRRE
                     GKLPPVTVRRHIEAARENARKKGANRQEVIVAGRQALRRLKARLRQEAAAAKKAAAAQ
                     AQQKADGN"
     gene            complement(3012..3452)
                     /locus_tag="VFB52_14065"
     CDS             complement(3012..3452)
                     /locus_tag="VFB52_14065"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO07511.1"
                     /translation="MASFTFERHVAAPPQTVFDVLTEHHLYAKITPMRKSVLEREGDP
                     PPNGVGAIRVLTSAGPPLREEVIAYEPPSRFSYTLLSGAPVRDHVGTVELHADGTGTR
                     MVYAVRTTPTLPVVGAAVVGVVKQAIKALIKGISTESERRAANG"
     gene            complement(3467..4465)
                     /locus_tag="VFB52_14070"
     CDS             complement(3467..4465)
                     /locus_tag="VFB52_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07512.1"
                     /translation="MAGKPRAEAPRAERILPGIWRLRLALPWPGVPHGNAWVLAAGDG
                     LVLVDTGFGGKHGLRDFELALAQVGFSVEDINLVVCTHAHSDHYGLAAPITAASGSEL
                     WIHPAWGHIRLMADDPEAALERRLEVARQSGVPAAGLETYRARNEDTETGIEGIREPD
                     RDLLPGVEVITDHGAWQVYETPGHAPSHVVLHQPDHKLMLTGDHLLGRTVLFFDYGHT
                     PDPVGEFLDSLDVVEPLGPNLCLPGHGRPFRDPELKIAAARKQVEDLRAKVREELGGE
                     QKTAFAIVAGILGPDNLSGPASGFLLQIVMALLDNMAVRGEVIAVEGSDPQRWAVA"
     gene            complement(4499..>5014)
                     /locus_tag="VFB52_14075"
     CDS             complement(4499..>5014)
                     /locus_tag="VFB52_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07513.1"
                     /translation="FFSTGGYSSVLQVVNGPREGADGSFGAGRWRWWAAEWGRLEWLS
                     AVVLFVGTLVFAINLVDSFLTELSPRQADRLIWSPDMVGCALFLVSGHLAFVELSGRW
                     YPSWQPRELGWWIVAVNQLGSVLFMVSAVASFVHHDGEMTSEAIANWGTLAGALCFAI
                     AGVLQEFERPA"
BASE COUNT          737 a         1763 c         1763 g          751 t
ORIGIN      
        1 tcctcgggcg ccgggccggc cgggctggtc gtctcgaagt tggtcttgct accgccgaag
       61 ccgtgcatca tcacgatcgt cggccagggc ccgttgccgg tcgccggcag ggtcacgtcg
      121 acgtcgagcg gcacgccatc gaaggagggc acccgttcgg ccggggtctg cgtggggcag
      181 aagcggacgc cgtcctgggc gacgcaggcg tgtccgaagg gggcgctcga ggaggccgcc
      241 gcgggcgcag cgagagcgag ggcgatgaca gggatcgccc agagcagcgg ccaggaccca
      301 gctctcctca tccagcagcg agcatacgcc tctcttcgcg tgttgggagg ggcatggccc
      361 ccgcgcgctt gaagaaaccc aagttccgaa tgacccggct ccgcgcccac atcaccgcgc
      421 tgatgtcgac cgctgcgatc gccgcggtcg cgattctcgt gctcgcggtc accgactcgg
      481 cgcaggcggc gccgctcgaa ccgccgggca agaagatcta cttcggcgtc tccgacaccg
      541 gcgacccggc cgacttcggc gccttcagca aagcgagcgg ccagcacccg gcggtgatcc
      601 agtccttccg tacctggggc gccgacttcc ccgagtcgat ccgccgctgg cagaccgccc
      661 gcgcccgccc gatgctccac atcagcaccg ccgacaacaa cgacggccac gagctgatca
      721 ccccggccca gatcgccgcc ggctacggcg acgagtacct gctgcgcctc aacaaactct
      781 tctggaacaa acgactggcc gcctacatcc ggccgctcgg cgagcccaac cgctgcctca
      841 acgtctacgc cgcctacgac tgcgagggca acttccgcga cgccgcccac agcccgcgca
      901 actaccgccg cgccttccgc cggatctaca tcctcgtcca cggtggcggc aaggcgcgca
      961 agatcaacgc ccgcctgcac gaagccggcc tccccccgct gaccgtcgac gtccccggcc
     1021 tgcccgccgc cccgatcgcg atcgtctgga gcccgctgcc cgccggctca ccgaccgtgc
     1081 ctcagaaccg cccccgccac ttctaccccg gcaacaagtg gaccgactgg gtcggcaccg
     1141 acttctacgc cgcctacccc gaatggaaag ccctcaacgg cgtctacaaa cgcttcaagg
     1201 gcaagccctt cgcgctcacc gagtggggcg tcgaacacgg cgacgacccg agcttcgtcc
     1261 gcggcctctt caactgggtc aaaagccacc cgcgcgcgga catgctggtc tactaccagg
     1321 acttcggctc cagctccagc taccggatcc agaactggcc ctcgagcctc agcgtgatgc
     1381 gcaaccagct ccgctcccgc ctcttccccc ccttcgccaa gggcttcccc cgcctccccc
     1441 cgcccccgcc gggcggcgtc ggcgcgggct agttcccgtc ggctttctgc tgcgcctggg
     1501 cggcggcggc ttttttcgcc gccgcggctt cttggcgcag gcgggctttg aggcgcctca
     1561 gggcctggcg gccggcgacg atcacttctt ggcggttggc gccttttttg cgggcgttct
     1621 cgcgcgcggc ttcgatgtgg cggcgcacgg tgaccggggg cagcttgcct tcgcggcgaa
     1681 gtttgaggcg gacggcgcgc aggcgtttgg ggttgtaggg ctgcagggcg gtgatcgagg
     1741 agtagccggt caggtagatg cggcggccgt cggagatcac cggcgtgtag gtgcccttcg
     1801 ggtactcgaa gacgcggcgg ccgctgcgca tcatgtagcc gctggtcgtt ttgttggtga
     1861 actcggcggc gtagacgatg tcgccgaccg cggagagcga gccgatcacc tggccgccgg
     1921 cgtcgcggct ccagcgcacg ctgccgtcct tggcgtcgag cgagtagatg ttgccgtcga
     1981 aggagccgat gaagatcgag ggtgggctgt ggcgggtgct ggcgacggtc ggccccgagt
     2041 agacgtagcc gccggtcgag taggtccagg cgagggtgcc gtccttcagc ccgtaggagt
     2101 agacgcggcc gtcgttgttg ccggcgtaga cgcggccgaa ggcgaccgcc ggggtcgaat
     2161 agaaggcgcc cgagccgccg aagcccggac cgagcgagcc gctctgccag atcagcttgc
     2221 cgctctccgc gtcgaccgcg ttcatgtggc cgccgtagtc gccgacgaac aggcgccccc
     2281 cgaagtaggc cggcgccgcc ttcacgtcgc cgcctagttc ggtgctccag cgctcgttgc
     2341 cgttgaccgt gctgagcgca tagaggcgcc cgttttcgca gccgaagtag acggtgttgc
     2401 cgatcaccag cggcgaggac tcggcccggt cgggcaggtc gcgcttccag atcgtgcgcc
     2461 cggttttggc gtcgagcttg aggatgtgcc cggggacgag gttgacgatg tagaggcggt
     2521 gtttggaata ggcgggcgag gaggcgttga gggtgccgat ccgccgcttc cacagcgtcc
     2581 gcccgttttt cgcgtcgagg gcgaaggcga agccgctgtt attgaccgcg tagagcgtgc
     2641 cgccgacgaa gatcggcggg aactcgagca gcggccggtc ggtgtagcgc cagagtttgc
     2701 ggaacggcgg cgccacccct ttcgccggca ggtagcgggt gcgggccggg ttcagcccga
     2761 acagcggcca gttcaccgtt ttcgccaccg gcggcggcgc tttcggtttg gtcgcttcga
     2821 aggcgacgtc ttcgttgtgg acgtcggccg gccgtttcag ctcctggtaa gcgaccacca
     2881 cagcgaagat cgccagcacc aggaccgcga tcccgaccag ggccagcgtc cgcttctctt
     2941 cccagaagcg ccgcgggtgc cgcaccaaca ggacgaaggt gtcgcggatg cggcgggcct
     3001 tgggcccgcc ctcagccatt cgccgcgcgc cgctcggact cggtcgagat gcccttgatc
     3061 agcgctttga tcgcctgctt gaccacgccg acgacggcgg cgcccaccac cggcagggtc
     3121 ggcgtcgtgc gcaccgcgta gaccatccgc gtgccggtcc cgtcggcgtg cagttcgacc
     3181 gtgccgacgt ggtcgcgaac cggcgccccg gagagcagcg tgtaggagaa gcggctgggc
     3241 ggctcgtagg cgatcacttc ctcgcgcagc ggcggccctg ccgaggtcag cacccggatc
     3301 gcgccgaccc cgttgggggg cgggtcgccc tcgcgctcga gcaccgactt gcgcatcggc
     3361 gtgatcttgg cgtacaggtg atgttcggtg agcacgtcga acaccgtctg tggcggggcg
     3421 gcgacgtgcc gctcgaaggt gaagctcgcc atacgtcgga gagaggctac gcgacggccc
     3481 agcgctgcgg gtcggacccc tcgacggcga tcacttcccc gcgcacggcc atgttgtcga
     3541 gcagggccat cacgatctgc agcaggaacc cgctggcggg cccgctgagg ttgtccggcc
     3601 cgaggatccc ggcgacgatc gcgaaggcgg tcttctgctc cccacccagc tcctcgcgca
     3661 ctttcgcgcg caggtcctcg acctgctttc gcgccgccgc gatcttcagc tcggggtcgc
     3721 ggaaggggcg gccgtggccg ggcaggcaga ggttcgggcc gagcggctcg accacgtcga
     3781 ggctgtcgag gaattcgccg accgggtcgg gggtgtggcc gtagtcgaag aacagcaccg
     3841 tgcgcccgag aaggtggtcg ccggtcagca tcagcttgtg gtcgggctgg tggaggacga
     3901 cgtgcgaggg ggcgtggccg ggggtctcgt agacctgcca ggcgccgtgg tcggtgatca
     3961 cctcgacccc gggcaggagg tcgcggtcgg gctcgcggat cccctcgatc ccggtctcgg
     4021 tgtcttcgtt gcgggcgcgg taggtctcca gcccggcggc ggggacgccg ctctgccgcg
     4081 ccacctcgag gcgccgctcc agcgccgcct ccgggtcgtc ggccatcagc cggatgtgtc
     4141 cccaggccgg gtggatccag agctcggacc ccgaggccgc ggtgatcggc gccgccagcc
     4201 cgtagtggtc ggagtgcgcg tgcgtgcaga cgacgaggtt gatgtcctcg accgagaagc
     4261 cgacctgggc gagcgccagt tcgaagtcgc gcagcccgtg cttgccgccg aagccggtgt
     4321 cgacgaggac gaggccgtcg ccggcggcga gcacccaggc attgccgtgc ggcacgcccg
     4381 gccacggcaa ggccaggcgc aggcgccaga tcccgggcag gatgcgttcg gcgcgcggtg
     4441 cctcagctcg tggcttcccc gccatctccg caatcctcgc agccgccgcg ggcgggggct
     4501 acgccggtcg ctcgaactct tgtaggaccc cggcaatcgc gaagcagagg gcgccggcca
     4561 gcgtgcccca gttggcgatc gcctctgagg tcatctcgcc gtcgtggtgg acgaaggagg
     4621 cgaccgcgga gaccatgaag aggaccgagc cgagctggtt gacggcgacg atccaccagc
     4681 ccagctcgcg cggctgccag ctcgggtacc agcggcccga gagctcgacg aaggcgagat
     4741 ggccggagac gaggaagagc gcgcagccga ccatgtccgg cgaccagatc agccggtcgg
     4801 cctggcgcgg ggacagctcg gtcaggaacg agtcgaccag gttgatcgcg aagaccaggg
     4861 tgccgacgaa gagcacaacc gcgctcagcc actccagcct cccccactcc gccgcccacc
     4921 agcgccagcg gccggcgccg aagctgccgt cggcgccctc gcgcgggccg ttgaccacct
     4981 gcaggaccga gctgtagccg ccggtgctga agaa
//
LOCUS       DASRIG010000525         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3643931, whole genome shotgun sequence.
ACCESSION   DASRIG010000525 DASRIG010000000
VERSION     DASRIG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643931"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1295)
                     /locus_tag="VFB52_14080"
     CDS             complement(147..1295)
                     /locus_tag="VFB52_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HZO07514.1"
                     /translation="MPAVEPFAVAITDAEIEDLRARLAATRWPREEPVDDWSQGVPTA
                     YVRELCEYWREGYDPRRLERRLNALPQFRTELDGVGTHFLHVRSPHPDALPLVLTHGW
                     PGSVLEFLAAVGPLTDPTAHGGEAEDAFHVVIPALPGYGFSDAPAETGWNPDRVGRAW
                     AALMSRLGYERYGAQGGDWGSAITRAVGRNDPEHVVGIHLNSDHVDRKVIAELGAESE
                     FERTALAQREAFNRDGRGYSREQATKPQTLGAGLADSPAGQCAWIVEKFAAWSDSGGD
                     PERAFDRDELLDNVTLYWLTNTAASSARMYWETFRAPSPVGPAGVPYALSTFPHDINP
                     VPERWIRAQDPDLVYYRLLPRGGHFAAMEVPDLFVPEVRAGFRAIRTA"
     gene            complement(1313..>2080)
                     /locus_tag="VFB52_14085"
     CDS             complement(1313..>2080)
                     /locus_tag="VFB52_14085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO07515.1"
                     /translation="WGWGSLPTLGLFAAGIAVALVWLKLEGVTEQPIIEVRLLRKPTV
                     LAVNVAAMLLGFAVYIGFLLIPQIAHAPTATGYGLGYSVSQASLMVLPLTGLQLCAGP
                     LASTVGKRIGFRATLILGTGLASVSLALLAGTHDQIWGLLVCGAGIGTGIAFAFAALA
                     NLVVDSVPPGDVGIATGINTVARTVGSAFGAAIATAILTSSLQASTGLPEGSGFTWAF
                     GAAAAFGVAATLAALRVPARRLEKGTGALAPIPQELP"
BASE COUNT          295 a          731 c          786 g          268 t
ORIGIN      
        1 cgggccgacc ttgctcgccg gtctgcgcga gcgcatccag ctcgagctcg tggatcgcca
       61 tgagttccgg tcgggggcgg tcgccctgcg ataccggtcc aggcaagctc cggcttgacg
      121 ttgttcacgg agaccggccg cagctgtcaa gcggtacgga tcgcgcggaa gccggcgcgg
      181 acctcgggga cgaagaggtc ggggacttcc atcgcggcga agtgaccgcc gcgggggagc
      241 agtctgtagt agacgaggtc ggggtcctgg gcgcggatcc agcgctctgg gacggggttg
      301 atgtcgtggg ggaaggtgct gagggcgtaa gggacgccgg cggggccgac cgggctggga
      361 gcgcggaagg tctcccagta catccgcgcc gaggaggcgg cggtgttggt cagccagtag
      421 agggtgacgt tgtcgagcag ctcgtcgcgg tcgaaggcgc gctcggggtc gccgccggag
      481 tccgaccagg cggcgaactt ctcgacgatc caggcgcact ggccggcggg tgagtcggcg
      541 aggccggcgc cgagcgtctg cggcttggtc gcctgctcgc gcgagtagcc gcgaccgtcg
      601 cggttgaagg cctcgcgctg ggccagcgcg gtgcgctcga actcgctctc ggcgcccagc
      661 tcggcgatca ccttgcggtc gacgtggtcg ctgttgaggt ggatgccgac gacgtgctcg
      721 gggtcgttgc ggccgaccgc gcgggtgatc gccgagcccc agtcgccgcc ctgcgcgccg
      781 tagcgctcgt agccgagccg ggacatcagc gccgcccagg cgcgcccgac ccggtccggg
      841 ttccagcccg tctccgccgg cgcgtcgctg aacccgtagc cgggcaaagc cgggatcacc
      901 acgtggaagg cgtcctcggc ctctcccccg tgcgcggtcg ggtcggtcag cggcccgacc
      961 gcggcgagga actcgagcac cgagccgggc cagccgtggg tgaggaccag cggcagcgcg
     1021 tcggggtgcg gcgagcgcac gtgcaggaag tgggtgccga cgccgtcgag ctcggtgcgg
     1081 aactggggca gcgcgttcag ccgccgttcc aaccgccgcg ggtcgtaccc ctcgcgccag
     1141 tactcgcaga gctcgcgcac gtaggcggtc gggacgccct gtgaccagtc gtcgaccggc
     1201 tcctcgcgcg gccagcgggt cgccgccagg cgcgcccgca ggtcctcgat ctcggcgtcg
     1261 gtgatcgcga ccgcgaacgg ctcgaccgct ggcatcgctc gatcctcctc gctcacggca
     1321 gctcctgcgg gatcggcgcc agggcgccgg tgcctttctc caagcgccgc gccggtaccc
     1381 gcagcgcggc cagcgtcgcc gcaaccccga acgcagccgc cgccccaaac gcccaggtga
     1441 acccgctgcc ctccggcagc ccggtcgagg cctgcagcga ggaggtcagg atcgcggtgg
     1501 cgatcgccgc gccgaaggcg ctgccgaccg tgcgggcgac ggtgttgatc ccggtcgcga
     1561 tcccgacgtc gccgggtggc accgagtcga ccaccaggtt ggccagcgcg gcgaaggcga
     1621 aggcgatccc ggtgccgatc ccggccccgc agacgagcag gccccagatc tggtcgtggg
     1681 tcccggccag cagcgccagc gagaccgagg cgaggccggt gccgaggatc agcgtcgccc
     1741 ggaagccgat ccgtttgccg acggtcgagg cgagcggccc ggcacagagc tgcagcccgg
     1801 tcagcggcaa caccatcagg ctcgcctggg agaccgagta gcccagcccg tagccggtag
     1861 cggtcggcgc gtgggcgatc tgggggatca gcaggaagcc gatgtagacg gcgaagccga
     1921 gcagcatcgc ggcgacgttc accgccagca ccgtcggctt gcgcagcaac cgcacctcga
     1981 tgatcggctg ctcggtcacg ccctcgagct tcagccagac cagcgccact gcgatcccgg
     2041 cggcgaacag cccaagcgtc ggcagcgacc cccagcccca
//
LOCUS       DASRIG010000526         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1550038, whole genome shotgun sequence.
ACCESSION   DASRIG010000526 DASRIG010000000
VERSION     DASRIG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550038"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFB52_14090"
     CDS             <1..272
                     /locus_tag="VFB52_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07516.1"
                     /translation="RTNRMGSDLACYMLERERVLELYERYAGWVPSEQSLHFWEVQKL
                     VFNTGLWLSGQANYLRGETDDLTLVRWSYTLPNMRRMVLDALEES"
     gene            269..709
                     /locus_tag="VFB52_14095"
     CDS             269..709
                     /locus_tag="VFB52_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07517.1"
                     /translation="MIEPERALRDVRAKLRETVLPQVGSTHGRTILAAALGIIDAVIE
                     QVKLDPAPAEATVAEMLPALVEWERELAVSDSGAADRLASARAQAERAADPFAAREQA
                     LAGAELALEAAWADPDPARRERLLASVRRAVRADVERQRRGGKG"
     gene            706..1482
                     /locus_tag="VFB52_14100"
     CDS             706..1482
                     /locus_tag="VFB52_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO07518.1"
                     /translation="MTEEVVLSERRDGVMVITLNRPEARNSLNLALTRALSDALDELD
                     ADPGLSAGVITGAGKGFCSGMDLKAFVAGENVWEGEEDDRGLRRIVTRAAQKPLLAAV
                     EGFAVAGGFELALACDLIVSGRSAKFGVPEVKRSLVAAGGALRQLPRRAGPGAAMKFA
                     LTGDLIDGERAYAIGLVDELSDNGGALDAALELAATIAANGPLAVAATKQILRDGGEW
                     SDEEFWARQKEICDPVFASRDATEGATAFAEKRAPVWSGK"
     gene            1482..2963
                     /locus_tag="VFB52_14105"
     CDS             1482..2963
                     /locus_tag="VFB52_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZO07519.1"
                     /translation="MAATLAERGAPAQTVREFWEQRVAATPDAPFLLHEGATETYAEL
                     DAAVNETANGLLAAGVEAGDHVALFLPSGRDLLLLELGLLKLGAVMVPMIDTLTAPEI
                     EYVVENSRAKMLISDPAAIVPGAAEPPPRPDLGPLDPMAIMYTSGSTGKPKGVVQPQA
                     GFASAGRAVSGRLATGPEDNYLCTIPIFHTAGFHMLVAAAIACGARLTLIPKFSRTEF
                     WNQVRASGATVGIVMPAQLSILMTAPPRPDDREHTLQRLATHVRPEAFCERFGVDVVT
                     VWAMTEVSGMGTMSEPGAGPVPEKCIGTAMPAEAEAKVVTAEGREAAPGERGELWYRH
                     PHAMSEYFRNPEATAASLVDGWVRTGDLCAADERGAIYFHGRIKNVIKRAGENVSGEE
                     VEATLVAHPDVAECVVLGVSDPIYTEEVYAAVSLRPGSAATAPELDAHCRERLAAWKA
                     PRYIALAEGELPKLANGKPDRLTIRAAADPASCWDRAASKEEG"
     gene            2960..3967
                     /locus_tag="VFB52_14110"
     CDS             2960..3967
                     /locus_tag="VFB52_14110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07520.1"
                     /translation="MTAPLSASLIVSRTPITTMVEMAREYEDHGFERVWLGEAWREPV
                     VPVTAMALGTERIGVGTAVSQIFPVNPVIVAQQAAQLQEVANGRFALGLGLGAAFVVE
                     RWFGVDPGRPLRRAREFIEIVRGALRSPAAGPFSYDGELLSTRKYTLPFVEEAVELPI
                     NLAAVGPKMQELAGELADGIVVGGLHSDEYMATMRERLRAGAERGDRDPEGLDVWFSL
                     TCCVDHDPEAARRQARRSLVYVAQYPHYQRVYESEGFGEVVAKIAGLVREREMDAAEA
                     LVSDVMIERFCLAGTPEECRKQLSRFEYGGAKPILHLVPFRASEEEVVESLRLAATLA
                     D"
BASE COUNT          578 a         1419 c         1480 g          516 t
ORIGIN      
        1 ggcggacgaa ccggatgggc tccgacctcg cctgttacat gctcgagcgc gagcgggtgc
       61 tggagctcta cgagcgctac gccggctggg tgccgagtga gcagagcctg cacttctggg
      121 aagtccagaa actggtcttc aacaccggcc tctggctcag cggtcaagcg aactatctgc
      181 gcggcgagac cgacgacctg accctggtcc gctggagcta caccctgccg aacatgcgcc
      241 ggatggtgct cgacgccctg gaggagtcgt gatcgagccg gagcgagccc tgcgcgacgt
      301 gcgcgcgaag ctgcgcgaga cggtcctgcc ccaggtcggc tcgacccacg ggcgcacgat
      361 cctcgccgcc gcgctcggga tcatcgacgc ggtgatcgag caggtgaagc tcgacccggc
      421 gccggccgaa gcgacggtcg cggagatgct gccggcactg gtcgagtggg agcgcgagct
      481 cgcggtcagc gactccggcg ccgccgaccg cctcgcctcc gcgcgggcgc aggccgagcg
      541 cgctgccgac cccttcgcgg cccgggaaca ggccctggcg ggtgccgagc tggcgctcga
      601 agccgcctgg gcggaccccg acccggcccg gcgcgagcgc ctgctggcgt cggtgcggcg
      661 ggcggtcaga gcagacgtgg aaagacaacg acgaggagga aaaggatgac cgaggaggtg
      721 gtgctgagcg agcgccggga cggggtcatg gtgatcacgc tgaaccggcc ggaggcgcgc
      781 aactcgctga acctggcgct gacccgggcg ctctccgacg cgctcgacga gctcgacgcc
      841 gaccccggcc tcagcgcggg cgtgatcacc ggcgccggca agggcttctg ctcgggcatg
      901 gacctgaagg cgttcgtcgc cggcgagaac gtctgggagg gcgaggagga cgaccgcggc
      961 ctgcgccgga tcgtcacccg cgccgcccag aagccgttgc tggcggcggt cgagggcttc
     1021 gccgtggccg gcggcttcga gctggctctg gcctgcgacc tgatcgtctc cgggcgctcc
     1081 gccaagttcg gcgtgcccga ggtgaagcgc tcgctggtcg ccgccggcgg cgccctgcgc
     1141 cagctgccgc gccgggccgg ccccggcgcg gcgatgaagt tcgccctcac cggggacctg
     1201 atcgacggcg agcgcgccta cgcgatcggc ctggtcgacg agctcagcga caacggcggc
     1261 gcgctcgacg cggcgctcga gctggcggcg acgatcgccg cgaacgggcc gctggcggtg
     1321 gccgcgacca agcagatcct gcgcgacggc ggcgagtggt ccgacgagga gttctgggcc
     1381 cgccagaagg agatctgcga cccggttttc gcctcgcgcg acgcgaccga gggggcgacg
     1441 gcgttcgccg agaagcgggc gccggtctgg tcagggaagt agtggccgcg actctcgcag
     1501 agcggggagc gccggcgcag accgtgcgcg agttctggga gcagcgcgtc gcggccacgc
     1561 ccgacgcgcc attcctcctg cacgagggcg cgaccgagac gtacgccgag ctcgacgcgg
     1621 cggtgaacga gaccgccaac ggcctgctcg ccgccggggt agaggccggt gaccacgtcg
     1681 ccctcttcct gcccagcggc cgcgacctgc tgctgctcga gctcggcctg ctgaagctgg
     1741 gggcggtgat ggtgccgatg atcgacaccc tgaccgcacc ggagatcgag tacgtggtcg
     1801 agaacagccg cgcgaagatg ctgatctcag atccggcggc gatcgttccc ggagcggccg
     1861 agccgccacc gcggcccgat ctcggcccgc tcgacccgat ggcgatcatg tacacgtcgg
     1921 gcagcaccgg caagccgaag ggcgtggtgc agccccaggc cggcttcgcc agcgccggcc
     1981 gcgcggtctc cgggcgcctc gcgaccgggc ccgaggacaa ctacctctgc acgattccga
     2041 tcttccacac cgccggtttc cacatgctgg tcgcggcggc gatcgcctgc ggcgcccggc
     2101 tcaccctgat cccgaagttc agccgcaccg agttctggaa ccaggtgcgg gcctcgggcg
     2161 ccaccgtcgg gatcgtgatg ccggcccagc tctcgatcct gatgaccgcg ccgccgcgcc
     2221 ccgacgatcg cgagcacacg ctgcagcggc tggccacgca cgtgcggccg gaggcgttct
     2281 gcgagcgctt cggggtcgac gtggtcaccg tctgggcgat gaccgaggtc tccgggatgg
     2341 ggacgatgag cgagccgggg gccggccccg tgcccgagaa gtgcatcgga acggcgatgc
     2401 ccgccgaggc ggaagcgaaa gtcgtcacgg ccgagggccg cgaggcggcg ccgggggagc
     2461 ggggcgagct ctggtaccgc cacccgcacg cgatgagcga gtacttccgc aaccccgagg
     2521 cgacggcggc gagcctggtc gacggctggg tcaggaccgg cgacctctgc gccgccgacg
     2581 agcggggcgc gatctacttc cacggccgga tcaagaacgt gatcaagcgc gcgggggaga
     2641 acgtctccgg cgaggaggtc gaggcgacgc tggtcgccca ccccgacgtc gccgagtgcg
     2701 tggtgctggg ggtcagcgac ccgatctaca ccgaagaggt ctacgccgcc gtctcgctgc
     2761 gccccggctc cgcggccacc gcccccgagc tcgacgccca ctgtcgcgag cgcctggcgg
     2821 cttggaaggc gccgcgctac atcgcgctcg ccgagggcga gctgccgaag ctcgccaacg
     2881 gcaagcccga tcggctcacg atccgcgccg ccgccgaccc cgcgtcctgc tgggaccgtg
     2941 ccgcctccaa ggaggaagga tgaccgcccc gctttccgct tccctgatcg tctcgcggac
     3001 cccgatcacg acgatggtcg agatggcccg cgagtacgaa gaccacggtt tcgagcgggt
     3061 ctggctcggc gaggcgtggc gcgagccggt cgtgccggtc accgcgatgg cgctcggcac
     3121 cgagcggatc ggggtcggga ccgcggtctc ccagatcttc ccggtcaacc cggtgatcgt
     3181 cgcccagcag gcggcgcagc tgcaggaggt cgccaacggc cgcttcgcgc tcggcctcgg
     3241 tctcggcgcc gccttcgtgg tcgaacgctg gttcggcgtc gacccggggc ggccgctgcg
     3301 ccgcgcccgc gagttcatcg agatcgtgcg cggcgcgctg cgctcgcccg ccgccgggcc
     3361 gttcagctac gacggcgagc tgctgagcac ccgcaaatac acgctgccct tcgtcgagga
     3421 ggcggtcgag ctgccgatca acctcgccgc ggtcggcccg aagatgcagg agctggcggg
     3481 cgagctggcc gacgggatcg tggtcggcgg cctgcactcg gacgagtaca tggcgacgat
     3541 gcgtgagcgg ctgcgggccg gagccgagcg cggcgaccgc gaccccgagg ggctcgacgt
     3601 ctggttctcg ctcacctgct gtgtcgacca cgaccccgaa gccgcccgcc gccaggcccg
     3661 ccgcagcctc gtctacgtcg cccagtaccc gcactaccag cgggtctacg agagcgaggg
     3721 cttcggcgag gtggtggcga agatcgccgg cctcgtgcgc gaacgcgaga tggacgcggc
     3781 cgaggccctg gtcagcgacg tgatgatcga gcgcttctgc ctcgccggga ccccggagga
     3841 atgccgcaag cagctatccc gcttcgagta cggcggcgcc aagccgatcc tccacctggt
     3901 ccccttccgg gccagcgagg aggaggtcgt cgagtccctg cggctggccg cgacgctggc
     3961 cgactagcta agcgtcgacc agggcggtcg ccc
//
LOCUS       DASRIG010000527         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_775364, whole genome shotgun sequence.
ACCESSION   DASRIG010000527 DASRIG010000000
VERSION     DASRIG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775364"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="VFB52_14115"
     CDS             complement(<1..1731)
                     /locus_tag="VFB52_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07521.1"
                     /translation="MHSIVQDFRFGLRALRRQPGFTAVAVLTLGLGLGATSAIFSVFR
                     AVLLAPLPYDQPERHVMIWSRWTGWDKTWVSSIEARDYGTNARLLQNVAAWSTDRVNL
                     TGGGEPERVGAGQVTANILAALGARPLIGHGFSPGDDASGDGAPVVLLGHGLWQRRFG
                     GDPAILGRTIQLDGRGFEVIGVMPSGFRLPTDYREDFAEPTELWTPLVLDTDPNDRGN
                     HGFYAAALLAPGATVPQANAELKGITSEWTKQGLYPEAMRFEAFAVSVADEILGPVTP
                     ALLLVSAATLFLLLIACANVANLLLARAESRRRELAVRSALGAGRFRLLRPLLAETLL
                     LAGGGGAMGLVLAFAGVRLLAGSSLAAIPRAQEARVDAAVLGFAALAALLTALVCSVV
                     PALRASRVDLTDSLKDGAGSVTIGRERQRSRSLLVVAEMALAVVLLVSAGLALRSLWA
                     LRQVRLGFDPEQVLTMRLALPQTSYETDAQVEGFYRDLVERVRTLPGVTQAGVVRSLP
                     LAATIGDWGLDVEGYVETPGDNAKGDWQVVSDGAFEALGERLEAGRFFTGADRADALP
                     VAVVNETMAKR"
     gene            complement(1789..>2149)
                     /locus_tag="VFB52_14120"
     CDS             complement(1789..>2149)
                     /locus_tag="VFB52_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07522.1"
                     /translation="GTSPTLVTSKSVYVSGEPIQVSWKNAPGSRWDWLGIYSPGESDS
                     SKVSSERVSGYGGNAHYRLYAYTRTAIEGTATFSAESFPGYAVWPLQPGNYEIRLLLD
                     DGYRSLASSPPFKVVKP"
BASE COUNT          316 a          784 c          778 g          271 t
ORIGIN      
        1 ccgcttcgcc atcgtctcgt tcacgaccgc gaccgggagc gcatcggccc ggtcggcgcc
       61 ggtgaagaag cggccggcct cgagtcgctc cccgagcgcc tcgaaggcgc cgtcgctgac
      121 gacctgccag tcccccttgg cgttgtcccc cggggtttcc acgtaacctt cgacatcgag
      181 cccccagtcg ccgatggtcg ccgccagggg cagcgagcgg acgacgccgg cctgcgtcac
      241 cccggggagc gtgcgtactc gctccacgag gtcgcggtag aacccctcca cctgcgcgtc
      301 ggtttcgtac gaggtctgag ggagcgccag ccgcatcgtc aggacctgct ccgggtcgaa
      361 cccgagacgc acctgccgca gcgcccacag gctgcggagc gccaggccgg ccgagacgag
      421 cagcacgacg gcgagcgcca tctcggcgac gacgaggagg ctacggctgc gctgccgctc
      481 gcgtccgatc gtgacgcttc ccgccccgtc cttgagcgag tcggtgaggt cgacgcgcga
      541 cgcccgcagc gccggcacga cgctgcagac gagcgcggtg agcagcgcgg cgagcgccgc
      601 gaagccgagg acggcggcgt cgacccgcgc ctcctgggcg cgcgggatcg cggcgaggct
      661 cgagccggcc agcaggcgca cgccggcgaa ggcgagcacc agccccatcg ccccgccgcc
      721 gcccgcgagc aggagcgtct ccgccaggag cggccgcagc agccggaagc ggccggcgcc
      781 gagcgccgat cgcaccgcca gctcgcggcg acgcgactcg gcgcgcgcca gcagcaggtt
      841 cgccacgttg gcgcacgcga tgaggagcag gaagagcgtg gcggcgctca cgagcaggag
      901 cgccggcgtc accggcccga ggatctcgtc cgcgacgctc accgcgaacg cctcgaagcg
      961 catcgcctcg gggtacagac cctgcttcgt ccactcggac gtgatcccct tcagctcggc
     1021 gttggcctga ggcacggtcg cccccggcgc cagcaacgcg gcggcgtaga agccgtggtt
     1081 gccgcgatcg ttcgggtcgg tgtcgaggac gagcggcgtc cacagctcgg tcggctcggc
     1141 gaagtcctcg cggtagtcgg tcggcaggcg gaagccggac ggcatcaccc cgatgacctc
     1201 gaagccccgc ccgtcgagct ggatcgtccg gccgaggatg gccgggtcgc cgccgaagcg
     1261 ccgctgccac agcccgtgtc cgaggagcac gaccggcgcc ccgtctcccg aggcgtcgtc
     1321 gccggggctg aacccgtggc cgatcagagg ccgggcgccg agcgccgcga ggatgttcgc
     1381 cgtgacctgg ccggcgccga cccgctccgg ctcgccgccc cccgtcaggt tgacccggtc
     1441 ggtgctccac gcggcgacgt tctgcagcag gcgggcgttg gtgccgtagt cgcgcgcctc
     1501 gatcgaggag acccaggtct tgtcccaccc ggtccagcgg ctccagatca tcacgtgccg
     1561 ttccggctgg tcgtagggga gcggcgccag cagcacggcg cggaacacgc tgaagatcgc
     1621 gctcgtcgcc ccgaggccga gtccgagggt gagcacggcg acggcggtga agcccggctg
     1681 ccggcgcagc gcccgcagcc cgaaacggaa atcctgaacg atggagtgca tcggccctcc
     1741 tccgatggga tctcagaagc ggaacgctgc ggcaggcgtc agggttcctt acggcttgac
     1801 caccttgaac ggcggggacg aggcgagcga gcggtagccg tcgtcgagca gcagccggat
     1861 ctcgtagttc cccggctgga gcggccacac ggcatagccg gggaacgact cggcggagaa
     1921 ggtcgccgtc ccctcgatgg cggtgcgggt gtaggcgtac agccggtagt gcgcgttgcc
     1981 gccgtacccc gagacgcgct cgctggacac cttgctgctg tccgactcgc cgggggaata
     2041 gatccccagc cagtcccagc gcgagccggg ggcgttcttc cacgacacct ggatcggctc
     2101 gccgctgacg tagacgctct tcgaggtgac gagggtcggc gatgtcccc
//
LOCUS       DASRIG010000528         5760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1015654, whole genome shotgun sequence.
ACCESSION   DASRIG010000528 DASRIG010000000
VERSION     DASRIG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015654"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFB52_14125"
     CDS             complement(<1..849)
                     /locus_tag="VFB52_14125"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07523.1"
                     /translation="MKRCVILLLIAVAALAPAAAAGAATQEREREPFFPRAGNAGYDA
                     TSYAVDLAFRPRTDRLQATATIEAIAAQRLSRFSLDLVGLTVDAVEVDGEPARFNRGR
                     EKLKIRPRTPIRPGNFFRVTVRYHGNPRTVTDPDGGIEGWIDTYDGALAVGEPVGTAA
                     WLPCNNVPADKAAFQVSLEVPSGLKAVSNGRLLEIEHGDGLTAYTWREPGPMSPYLAL
                     INIGRGKLRAGTIAGRPSWTLIDPVLERRSLPVLAKLSEVIRFEEKIFGRYPFETAGS
                     VVDFAPA"
     gene            873..1625
                     /locus_tag="VFB52_14130"
     CDS             873..1625
                     /locus_tag="VFB52_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO07524.1"
                     /translation="MAAARVDYDRHGLTYTRNRQADPRIEARVHAALGDAETVLNVGA
                     GTGSYEPCDRWVLAVEPSATMRALRPPDAAPALAARAEALPFDDDSFDAAMACITVHH
                     WEPPAAGLAELRRVARGPVVVLTFELDALAAWQREFLAEGLEIERPRFGPAEDVAAAL
                     GGRTRVETIPTPADCADGFFEAFWNRPEALLDPRVRASQSMWELLDDGVEAKIVERLQ
                     AALDSGAWDAEHGHLRAQESFDGSLRLVISER"
     gene            complement(1837..2826)
                     /locus_tag="VFB52_14135"
     CDS             complement(1837..2826)
                     /locus_tag="VFB52_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07525.1"
                     /translation="MKRLLLLAATCCALLVPGNAGAATIVNGDFETGNLSGWTVFNST
                     SKGNWFPYAGTQVPFANPEEQFFAPPQGNWAAVTSETSPDLAILFQDVTLEPFWTHNL
                     SLIYYYRSFAPIAIPTPYTLDITEELVKMAEPPLGEDQENQQLRIDVIKPTAPIDTVN
                     PAEILTTIFANKVGDPQVIPPTTLSADLTQFAGQTVRLRIANAVNEEVFNAAVDAVTL
                     TSTPPSNVFTKGKLQLNKKKGTAKLAINVPGAGVLKIANGGKGKKAPKLIKAATKNPT
                     KAGKVWVPIKPTGTGKATLTEDGKLQFKVKVTFTPTGGTAATKTFGGKLKLND"
     gene            complement(2828..3799)
                     /locus_tag="VFB52_14140"
     CDS             complement(2828..3799)
                     /locus_tag="VFB52_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07526.1"
                     /translation="MSRPKSLGKLLALAFTVCALLAPSAASAATVVNGDFETGNLSGW
                     TLVNTTFNGSWFPYAGTKTPFADGGDPPFFAPPQGNWAAVTDEVEPVTAVMYQDIALE
                     PGWTHSISATLYYLSFAPINAQPTLLTPTGGPEGPQQYRVDVIKPTAPLLTLAPEDIL
                     ATLFANKFGDPQVMAPTAFSADLSALAGQTVRLRLVNAVNNSVYNAGADAISITSTPP
                     SNVFTKGKLQLNKQNGTAKLAINVPGAGTLKVANGGKGKAPKLIKAATKNPTKAGKVW
                     LPIKPTGTAKTKLKEKGKLAFKVKVTFTPTGGFAATKNFGGTLKLED"
     gene            complement(3856..4464)
                     /locus_tag="VFB52_14145"
     CDS             complement(3856..4464)
                     /locus_tag="VFB52_14145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZO07527.1"
                     /translation="MPDRPEPPADPFGYDAVLVLGGAMNVDQGEKHGWLAGEKALLAE
                     LLERERPLLGLCLGSQLVAEAAGAEPRRASRPEIGWHPVELTAEGAADPLLAPLAPGF
                     EAFQWHSYEFPLPPGAVALARSEVCLQAARVGKAAWALQFHPEVSKADALWWIEDYRS
                     DPDAVALGVDPAVLAAETEGKIGGFNQLGRDICLRWLAAAGA"
     gene            4631..>5760
                     /locus_tag="VFB52_14150"
     CDS             4631..>5760
                     /locus_tag="VFB52_14150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZO07528.1"
                     /translation="MNPYLENGREVVIVEAVRTPVGRGHLEKGYYKDLHPSTLLARTL
                     AELVNRAGIDPAEVGNIVGGCVSQFGEQSLNITRNAWLEAGLPETVPAQTLDIQCGSG
                     QQSVNFAAALISSGAHDIAIGCGVESMSHNSFADGAQVMKEQGFPFSPALLERYNLIP
                     QGLSAEMIAEQWEIPRSELDEIGLRSQQNAAKATEEGRFEREILPMQVNGDTYVTDQG
                     IRPETTIEALAGLKPAFKEDGTITAGNSSQVSDGAAAVLLMTREKAEALGLTPRARIY
                     DQVTVGVDPVIMLTGPIPATEKILERSSLKMDDVDLVEINEAFASVVGAWQRELKPDM
                     DRVNVNGGAIALGHPLGSTGARLTTSLLHELERSDKSMGLVT"
BASE COUNT          843 a         1924 c         2037 g          956 t
ORIGIN      
        1 cgcgggggcg aagtcgacca ccgagccggc ggtttcgaac gggtagcggc cgaagatctt
       61 ttcctcgaac cggatcacct cggagagctt tgccaggacc ggcagcgagc gccgttcgag
      121 caccgggtcg atcagcgtcc agctcggccg gccggcgatc gtgccggcgc gcagcttgcc
      181 gcggccgatg ttgatcagcg ccaggtaggg gctcatcgga ccgggttcgc gccaggtgta
      241 ggcggtcagc ccgtcgccgt gctcgatctc cagcaggcgc ccgttggaga ccgccttcag
      301 cccgctcggc acctccagcg agacctggaa cgcggccttg tcggcgggga cgttgttgca
      361 gggcagccaa gcggcggtgc cgaccggctc gccgaccgcg agcgcgccgt cgtaggtgtc
      421 gatccagccc tcgatcccgc cgtcggggtc ggtgacggtg cgggggttgc cgtggtagcg
      481 gaccgtgaca cggaagaagt tgccgggccg gatcggggtg cgcgggcgga tcttcagctt
      541 ctcgcggccg cggttgaagc gcgccggctc gccgtcgact tcgaccgcgt cgacggtcag
      601 ccctaccaag tcgagggaga agcgcgagag tcgctgagcg gcgattgctt cgatcgtcgc
      661 ggtcgcctgc aggcggtcgg tgcgcggccg gaaggcgagg tcgacggcgt aggaggtggc
      721 gtcgtagccg gcgttcccgg cgcgcgggaa gaacggttct cgctcccgct cctgggtcgc
      781 ggccccggcg gccgcggccg gcgcaagggc ggcgacggcg atcaggagca ggatcacaca
      841 gcgcttcacc ggtcccatgt taggttcgcc cggtggcggc agcgcgggtg gactacgacc
      901 ggcacggcct gacttacacg cggaaccggc aggcggaccc gcggatcgag gcgcgtgtgc
      961 acgctgctct cggcgacgcc gagaccgtgc tcaacgtcgg cgccgggacg ggttcttacg
     1021 agccgtgcga ccgttgggtg ctggcggttg agccgagcgc gacgatgcgc gcgctgcgcc
     1081 cgcccgatgc cgcgccggcg ctcgccgccc gcgccgaggc gctgcccttc gacgacgaca
     1141 gcttcgacgc cgcgatggcc tgcatcaccg tccaccactg ggaaccgccc gccgccggtc
     1201 ttgccgaact gcgccgcgtc gcccgcggcc cggtcgtggt gctcaccttc gagctcgacg
     1261 cgctcgccgc ctggcaacgg gagttcctcg ccgagggcct ggagatcgag cgcccccgct
     1321 tcggcccggc cgaggacgtc gccgccgcac tcggtggccg cacccgagtc gagacgatcc
     1381 cgacccccgc cgactgcgcc gacggcttct tcgaggcctt ctggaaccgc cccgaggccc
     1441 tgctcgaccc tcgtgtccgc gcctcgcagt cgatgtggga gttgctcgac gacggcgtcg
     1501 aggcgaagat cgtcgaacgc ctccaagccg ccctcgactc cggcgcctgg gacgccgagc
     1561 acggccacct ccgagcgcaa gagagcttcg acggctcgtt gcgcttggtt atatccgaac
     1621 ggtaggggga gacaaacgct ccctatcgac tttgttcaga cgtgtcgaac aaactcgata
     1681 gggccaccct gggcctcgca cagatcgcgc aacggcagca cggagtggtg agcgcagcac
     1741 agctcaccgg gcttgggatc tcgcgggcaa cggtctcggg gtgggtgagg cgcgggcgcc
     1801 tgcaccgccg cgtccacgcg aagtcctggg cggcggctag tcgttcagct tcagcttgcc
     1861 gccgaacgtc ttagtcgccg cggtgccgcc ggtcggggtg aaggtgactt tgaccttgaa
     1921 ctgcagcttg ccgtcctcgg tcagcgtcgc cttgcccgtg ccggtcggct tgatcggcac
     1981 ccagactttg ccggctttcg tcgggttctt ggtcgccgcc ttgatcagct tcggggcctt
     2041 tttgcctttg ccgccgttgg cgattttgag cacgcccgca cccggcacgt tgatcgcgag
     2101 cttggccgtg ccctttttct tgttcagctg caatttgccc ttggtgaaga cgttcgaggg
     2161 cggggtgctg gtcagggtga ccgcgtcgac agcggcgttg aagacctcct cgttgaccgc
     2221 gttggcgatc cgcaggcgca cggtctggcc ggcgaactgg gtcaggtcgg cgctcagcgt
     2281 ggtcggcggg atcacctgcg ggtcaccgac tttgttggcg aagatcgtgg tgaggatttc
     2341 cgctgggttg acggtgtcga tcggcgccgt cggcttgatc acgtcgatcc gcagctgctg
     2401 gttttcctgg tcttcaccca gcggcggctc ggccattttc acgagttcct cggtgatgtc
     2461 gagcgtgtag ggcgtcggga tcgcgatcgg cgcgaacgag cggtagtagt agatcagcga
     2521 gaggttgtgg gtccagaacg gctccagggt cacgtcctgg aagaggatcg ccaggtcggg
     2581 gctggtctcg ctggtgaccg cagcccagtt gccctgaggc ggcgcgaaga actgttcttc
     2641 cggattcgcg aaggggacct gggtgccggc gtaggggaac cagttgcctt tcgacgtgct
     2701 gttgaagacg gtccagccgc tcaggttgcc ggtctcgaag tccccgttga cgatcgtcgc
     2761 ggcgccggcg ttgccgggga ccagcagcgc gcagcaggtg gcggcaagga gcagcaggcg
     2821 tttcatttca gtcctccagc ttcagcgtgc cgccgaagtt cttcgtggcg gcgaagccgc
     2881 cggtgggggt gaaggtgact ttgaccttga aggcgagctt gcccttctcc ttcagcttcg
     2941 tcttcgcggt cccggtcggc ttgatcggca gccagacttt gccggctttc gtcgggttct
     3001 tggtcgccgc cttgatcagc ttcggcgctt tgcctttgcc gccgttggcg actttcaggg
     3061 tgccggcccc cggcacgttg atcgccagtt tcgcggtccc gttctgcttg ttcagctgca
     3121 gcttgccctt ggtgaagacg ttcgagggcg gggtgctggt gatcgagatc gcgtcggcgc
     3181 cggcgttgta gacgctgttg ttgaccgcgt tgaccagccg cagccgcacg gtctgaccgg
     3241 cgagcgcgct caggtcggcg ctgaacgcgg tcggcgccat cacctgggga tcgccgaatt
     3301 tgttcgcgaa cagcgtggcg aggatgtctt ccggtgccag ggtcagcagc ggcgcggtcg
     3361 gtttgatcac gtcgacccgg tactgctgcg ggccttccgg ccctccggtc ggcgtgagca
     3421 gcgtgggctg ggcgttgatc ggcgcgaagc tgaggtagta gagcgtggcc gagatgctgt
     3481 gcgtccagcc cggctccagg gcgatgtcct ggtacatgac cgcggtcacc ggttctacct
     3541 catcggtgac cgccgcccag ttgccctgcg ggggtgcgaa gaagggggga tcgcctccgt
     3601 ccgcgaacgg ggttttggtc ccggcgtacg ggaaccagct gccgttgaaa gtcgtgttga
     3661 cgagcgtcca gccgctcagg ttgccggtct cgaagtcccc gttgacgacg gtcgcggcgc
     3721 tggccgcgga cggggcgagc agggcgcaga cggtgaaggc aagcgcgagc agcttgccga
     3781 gggacttcgg acgggacatg cggggagacc tcctgatcga cagcgaaccg cgaggaatcg
     3841 tagtcgcgaa gccggtcagg cgccagcggc ggcgagccag cgtagacaga tgtctcgccc
     3901 gagctggttg aagccgccga tcttgccttc ggtctccgcc gccagcaccg ccggatcgac
     3961 cccgagcgcg accgcgtcgg ggtcggagcg gtagtcctcg atccaccaga gcgcgtccgc
     4021 cttgctcacc tcggggtgga actgcagggc ccaggcggcc ttgccgaccc gcgccgcctg
     4081 caggcagacc tcgctgcggg cgagcgcgac ggcgccgggc ggcaggggga actcgtagct
     4141 gtgccactgg aaggcctcga agccaggggc gagcggggcg agcagcggat cggcggcgcc
     4201 ctccgcggtc agctcgaccg ggtgccagcc gatctccggg cgggaggcgc ggcgcggctc
     4261 ggccccggcc gcttcggcga ccagctggct gccgaggcag aggccgagca gcggccgctc
     4321 gcgctcgagc agctcggcca gcagcgcctt ctcccccgct agccagccgt gcttctcccc
     4381 ctgatcgacg ttcatcgcgc cgccgaggac cagcaccgcg tcgtagccga acgggtccgc
     4441 gggcggctcc gggcggtccg gcaccagcca cctgtcgacc tctccaccgg ccgcgactat
     4501 cgcttccgcg aaaaccccgg gaccggcgtc atcctgatgg acaatggcga gcacacgcat
     4561 cccccaagtt tcccagttga cttgcttact atcatgtaag taaggacgtg aaacgcgaaa
     4621 ggaccttcac atgaacccct atctggaaaa cggccgcgag gtcgtgatcg tcgaggcggt
     4681 gcgcaccccc gtcggccgcg gacacctcga gaagggctac tacaaagacc tgcacccctc
     4741 gaccctgctg gcgcggacgc tcgcggagct ggtcaaccgg gccgggatcg atcccgccga
     4801 ggtcggcaac atcgtcggcg gctgtgtcag ccagttcggc gagcagagcc tcaacatcac
     4861 ccgcaacgcc tggctcgagg ccggtctccc ggagaccgtc cccgcccaga cgctcgacat
     4921 ccagtgcggc tccggccagc agtcggtcaa cttcgccgcc gcgctgatct cctccggcgc
     4981 ccacgacatc gcgatcggtt gcggggtcga gagcatgagc cacaactcct tcgccgacgg
     5041 cgcccaggtg atgaaggaac agggcttccc gttctcgccg gccctgctcg agcgctacaa
     5101 cctgatcccg cagggcctct cggccgagat gatcgccgag cagtgggaga tcccccgctc
     5161 tgagctcgac gagatcggcc tgcgctccca gcagaacgcc gcgaaggcga ccgaggaggg
     5221 ccgtttcgag cgtgagatcc tgccgatgca ggtcaacggc gacacctacg tcaccgacca
     5281 gggcatccgt ccagaaacga cgatcgaggc cctcgccggg ctgaagccgg cgttcaaaga
     5341 ggacggcacg atcaccgccg gcaactcctc gcaggtctcc gacggcgccg ccgcggtgct
     5401 gctgatgacg cgcgagaagg cggaggcgct cggcctcacc ccgcgggcgc ggatctacga
     5461 ccaggtcacg gtcggggtcg acccggtgat catgctgacc ggcccgatcc cggcgaccga
     5521 aaagatcctc gagcgcagct cgctgaagat ggacgacgtc gatctggtcg agatcaacga
     5581 ggcgttcgcc tcggtggtcg gcgcctggca gcgtgagctg aagccggaca tggaccgggt
     5641 caacgtcaac ggcggcgcga tcgcgctcgg ccacccgctc ggctcgaccg gcgcccgcct
     5701 gacgaccagc ctgctgcacg agctcgagcg ctccgacaag agcatgggcc tggtgacgat
//
LOCUS       DASRIG010000529         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3645990, whole genome shotgun sequence.
ACCESSION   DASRIG010000529 DASRIG010000000
VERSION     DASRIG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645990"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="hflX"
                     /locus_tag="VFB52_14155"
     CDS             <1..863
                     /gene="hflX"
                     /locus_tag="VFB52_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZO07529.1"
                     /translation="GLWTHLERLGAGRMDGGIGTRGPGESQIETDRRLARNRIAALRR
                     RLTRLERNRGVMRARRERSSLPRVALAGYTNAGKSTLLNALTGATVGVGDRLFHTLDP
                     TTRTLELSGRDYLLTDTVGFIEKLPHQLVEAFKATLEETTLADLIVHVVDASEPEERR
                     LVDIKAVDEVLEEIGAGEKQRLLVLNKADLLDEDARHEVTIAHPGAVLVSALADEGLD
                     TLREAIETAFAETLTEVELLIPYSQGGRLHELHEVAGDLERTEREDGVLVHARVPAAE
                     LHRFTDLAVA"
     gene            892..1323
                     /gene="dut"
                     /locus_tag="VFB52_14160"
     CDS             892..1323
                     /gene="dut"
                     /locus_tag="VFB52_14160"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZO07530.1"
                     /translation="MELPVVKLKDEAVLPSRAHPGDAGLDLYACEAAHIGPGERWGVG
                     TGVAVEIPEGFAGLVLPRSGLAREHGITLVNSPGLIDAGYRGELRVLLLNADPADIFR
                     VEPGDRIAQLVLTPIALAEPVEAAELSESSRGEGGFGSSGR"
     gene            1541..2203
                     /locus_tag="VFB52_14165"
     CDS             1541..2203
                     /locus_tag="VFB52_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07531.1"
                     /translation="MIHRGKVLGLLLAVALATGAFTAVAAQAEPLFEANSYPATITGD
                     QVPGEELVLTVEKGLSTECETRHFHALISEATSSVTATTEQAGCKAFGFITATVNQNG
                     CEFRFDVTEKLATDKYVGNADFLCPEGKKMQVQTATCQIEVGSQENLGPIYFENSTEA
                     GHITLRPEIKEKITYTKTKDPLFCPFNGTGTKSDGSFVGNTTVEGEVVGGGSAIGIKV
                     DG"
     gene            complement(2238..>2776)
                     /locus_tag="VFB52_14170"
     CDS             complement(2238..>2776)
                     /locus_tag="VFB52_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZO07532.1"
                     /translation="LVAEDMLRTKRDAIASAVDDGAALLAVCGGYQLLGHSYQLGEEK
                     LPGLGLADLETVREPGPRLIGNVAIEVDLGAGPRQLAGFENHGGRTYLGTAAEPLGRV
                     LEGHGNNGGDGLEGVRRANLIGTYLHGPLLPKNAWLADHLIATALERRYGTRPELEPL
                     DDAFEDAAHASARAAAGV"
BASE COUNT          465 a          949 c          969 g          393 t
ORIGIN      
        1 gggggctctg gacccacctc gagcgcctcg gcgccggcag gatggacggc ggcatcggca
       61 ccaggggccc cggcgagagt cagatcgaga ccgaccgccg cctggcccgc aaccgcatcg
      121 ccgccctgcg cagacgcctg acgcggctcg agcgcaaccg cggcgtgatg cgcgcccgtc
      181 gcgagcgttc ctcgctgcct cgcgtggcgc tcgccggcta caccaacgcc ggcaagtcga
      241 cgctgctgaa cgcgctcacc ggtgccaccg tgggagtggg ggaccggctc ttccacacgc
      301 tcgacccgac cacccgcacg ctcgagctct ccggccgcga ctacctgctc accgacaccg
      361 tcggcttcat cgagaagctc ccccaccaac ttgtcgaggc gttcaaagcg acgctcgagg
      421 agaccacgct cgccgacctg atcgtgcacg tggtcgacgc ctccgagccg gaggagcgcc
      481 gactggtcga catcaaggcg gtcgacgagg tgctggagga gattggcgcg ggggagaagc
      541 agcggctgct ggttttgaac aaggccgacc tgctcgacga ggacgcccgc cacgaggtga
      601 cgatcgccca tcccggcgcg gtgctcgtct cggcgctcgc cgacgaaggg ctcgacacac
      661 tgcgcgaggc gatcgagacc gccttcgccg agaccctgac cgaggtcgag ctgctgatcc
      721 cgtactcgca gggcggccgc ctgcacgagc tgcacgaggt ggccggcgac ctggagcgga
      781 ccgagcgtga ggacggcgtc ctcgtccacg ctcgcgttcc cgccgcagag ctgcaccgct
      841 tcacagacct cgccgtcgcg tagtcgcgag cgctcagtag gctgccgggc cgtggagctt
      901 cccgtggtca agctgaaaga cgaggcggtc ctgccgagcc gtgcccatcc gggcgacgct
      961 gggctcgacc tctacgcctg cgaggccgcc cacatcggtc ccggtgagcg ctggggtgtc
     1021 ggcacgggcg tcgcggtcga gatccccgag ggcttcgccg gattggtgct gccgcgctcc
     1081 ggcctggccc gcgagcacgg gatcaccctg gtcaacagcc ccgggctgat cgacgccggc
     1141 taccgcggtg aactccgggt tctgctgctg aacgccgacc cggcggacat cttccgggtc
     1201 gagccgggcg accggatcgc gcagctggtg ctgaccccga tcgcgctcgc cgagccggtc
     1261 gaggcggctg agttgagcga gtcctcgcgc ggcgagggcg gcttcggctc cagcgggcgc
     1321 tagttccgca cggatcggcg cgcgcgggaa atggcgcgcg cgcaaccctg cggatctcgc
     1381 tgtgcgggtg aattggtgcg atgggcgtcc tctcgcccgc gattgatcgc tccgagaccg
     1441 ccaaatcggc cccgaccgcg tgctgaatgg ctaggaaatc cccgcccgta ccggttcggc
     1501 cccggggcgg atcgatagtc aatgactaag ggggaaacag atgattcacc gaggcaaggt
     1561 gctcgggttg ctgctggctg tggcgctggc gaccggcgcg ttcacggcgg tcgccgcgca
     1621 ggcggaaccg ctgtttgaag ccaacagtta cccggcgaca atcacaggcg accaggtgcc
     1681 gggcgaagag ctggtgctga cggtcgaaaa aggactgagc acagagtgcg aaacgcggca
     1741 cttccacgcg ctcatctcgg aagcgacgag cagcgtcacc gcaaccaccg aacaggcggg
     1801 ctgcaaagcc ttcgggttca tcaccgcgac ggtcaaccag aacggctgtg aattccgttt
     1861 cgacgtgacc gaaaaactcg cgaccgacaa atacgtgggc aacgctgact tcctctgtcc
     1921 ggaaggcaaa aaaatgcagg tccagaccgc gacctgtcag atcgaagtag gcagccagga
     1981 aaacctcggc ccgatctact ttgaaaactc gacagaagcc ggccacatca cactgcggcc
     2041 ggaaatcaaa gaaaaaatca cctacacgaa aacgaaagac cccctgttct gcccgttcaa
     2101 cggcacgggt acgaaaagcg acgggtcgtt cgtcggcaac accacagtcg aaggcgaggt
     2161 cgtcggcggc ggcagcgcga tcggcatcaa agtcgacggc tgagcgagcg agggccggga
     2221 gcgatcccgg ccctttctca caccccggcg gcggcgcggg cgctggcgtg ggcggcgtcc
     2281 tcaaacgcgt cgtcgagcgg ctccagctcg ggacgggtgc cgtagcggcg ctccagcgcg
     2341 gtcgcgatca ggtggtcggc gagccaggcg ttcttcggca gcagcgggcc gtgcaggtag
     2401 gtgccgatca ggttggcccg ccgcacgccc tcgagcccgt cgccgccgtt gttgccgtgg
     2461 ccctcgagga cgcggccgag gggctctgcg gcggtgccga gataggtgcg tccgccgtgg
     2521 ttctcgaagc cggcgagctg ccgcgggccg gcgccgaggt cgacctcgat cgcgacgttg
     2581 ccgatcagcc gcgggcccgg ctcgcgcacc gtctcgaggt cggccaggcc gaggccgggc
     2641 agcttctcct cgccgagctg atagctgtgg ccgagcagct ggtagccgcc gcagacggcg
     2701 agcagggcgg cgccgtcgtc gacggcgctg gcgattgcgt cgcgcttggt ccgcagcatg
     2761 tcctcggcga ccagct
//
LOCUS       DASRIG010000530         6531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1807406, whole genome shotgun sequence.
ACCESSION   DASRIG010000530 DASRIG010000000
VERSION     DASRIG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807406"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1867)
                     /locus_tag="VFB52_14175"
     CDS             complement(41..1867)
                     /locus_tag="VFB52_14175"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO07533.1"
                     /translation="MEPSESTSRRSSAAEIAQSWVDALLASAAEGDLERAGSLFAEGG
                     TWRDLLGFQWDFKNWTGGQEVAAALLEALAAVPVGDLTVRTDREPRFEEDGSQVTLFI
                     RFETERGPSEGFVRLGGDAEGGWKCLYLLTQLQELAGFPEQSGSHRPLGKDHTITPDR
                     IDWATAREAERDFAEAQPAVVILGAGHGGLALAARLGAHGIPTLLIERNRRVGDNWRN
                     RYPSLALHDPVGADHMPYLHFPDTWPKFTPKDKFANFLEYYVDALDLNVWTGSNVVAA
                     EFDGDEQRWALTVTREDGAERVLAPRHFVIATGLNGNPNLPAIAGSEEFEGTLVHSGD
                     YPGGARWKGKKALVVGSGVSGHDVIQDLYESGAEVTLLQRSATYVINGETFHNINFAN
                     YLEGSTEELLDADLIAASIPWGQFMPLGKMVTDMAREQDAELLEALQGQGFAIEYGPE
                     EAGVVVLHIAGRDGYQINMGASDLIADGRVGVRSGVTIDRLTPAAAVLSDGSSLDADV
                     IVMATGYGKILGMVRPLLGELADNVRPIYQVGEDGELSVVWRRCGQDGLWFMTGIIQT
                     ARYYSQLLALQIEAIERERLSYREADQPRGFTAAGTAASVVA"
     gene            complement(1950..2981)
                     /locus_tag="VFB52_14180"
     CDS             complement(1950..2981)
                     /locus_tag="VFB52_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07534.1"
                     /translation="MGAKRAPTEVCIVATPEAMVSPVSGLYETLAAVGGLESPETQRA
                     GGEPFAVKIVAERAGPVRSPSGLSIAPDATIDQVDRTDVVIVPSMAMTEDHSWEVGRH
                     PHLVAWLRRMYEGGATLCSACTGGMLTAETGLLDGQEATAHWITVTPFRRRHPEVRLR
                     PEEALVVSGEDGRLVTSGASTAWHDLLLYLVARYVGPATAQALARFQLVQWHSDGQDA
                     FASFEPPTDHEDAAVLAAQRWIAANYAVAAPVEEMAAVAELAPTTFKRRFKAATEMTP
                     IAYVQQVRVEKAKRRLETSADPVEEIAWAVGYDDPAAFRRLFKRTTRLTPGAYRRRFR
                     LPELEGVQR"
     gene            3098..4171
                     /locus_tag="VFB52_14185"
     CDS             3098..4171
                     /locus_tag="VFB52_14185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZO07535.1"
                     /translation="MNPQTIELDEARVEAFNAEVIGQLAACYGGVMIRLGEQLGLYAA
                     LHGAGPLTSAELAARSGCAERYVREWLGSQVASGALDYEAGAETYELSPEHGLVLADP
                     DSPAYMASAFGVAATLWADEAKAVRAFRTGTGVPWGEHDERLFCGVASFYRNAYAASL
                     VPEWLPALEGTVAKLEAGARVADVGCGHGHSTVLMAEAFPASSFVGIDVHADSIAHAR
                     ANAEAAGVADRVEFVVGSASEYGRNGFDLICFFDAFHDLGDPYGAAAEARRALAADGQ
                     VMLIEPFAADGVEGALGPVAQLYYAASTTLCCAHAISEGAEDPLGAQAGEDRLRAVFE
                     EAGFGAWRRAAETPFNLVLEARA"
     gene            4503..>6531
                     /locus_tag="VFB52_14190"
     CDS             4503..>6531
                     /locus_tag="VFB52_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZO07536.1"
                     /translation="MENSQLSSPASEQAFVGVDVGGTHTDVQVIMGEREARGKALTSY
                     DDFSRGVLGAVGVAAENLGITLEELLSRTQLLVNATTVVTNSITQLKGAEVGILVTNG
                     FKDEFRFAGGPRIRDVDDQIQTNVPDIVKRRNVLEVRERMQFSGEAAVPLNEDDVLSQ
                     ARLLVDRGVSAIAICFLNSYSNGAHEQRAAELIEDEFPQIFVTPSHRVSSVRGEYPRW
                     MTAVLNCFVHEHAKRFLDALGTKLHEAGLRGHVAFFQGLGGGVSQGRAEQLPLTLLGA
                     GPAGGATGSNELARRMGHDRVLLGDMGGTSFDTGLIHDNVVHIEKDIQVGRFKTAMPL
                     VDVISVGAGGGSIAWISERGAPQVGPQSAGSSPGPAAYNNGGTEPTVTDAMIAMDLID
                     PANYLGGRVELRRDLAIEALRTRFAEPMGWSVEEGAAAVHDLVVANMANAVREVSVAH
                     GHDPREFVFYAYGGTLPWFAAQIAEVLQIGTVLVPHSSSVFCARGLLVSDFVLRNDQT
                     LQSGLAGEGEVERINEAARSLVAVGQESMRSEGFGDEEIEIARSGDFQFAGQVHALPM
                     PLPDRELSGDDVPMLQDQFNQVYERTYGKGTAWPGVPPQLLNLSVTVTGKLPRPPIAE
                     RPLDPREPAEMLRGEREVYLPTERKRETIPIYDETKVTPGSRVEGPALVEAV"
BASE COUNT          945 a         2293 c         2301 g          992 t
ORIGIN      
        1 cgaggtcgac ctgcgggtag atgactccga gcttggtcat ctaggccacc acgctcgcgg
       61 ccgttccggc ggccgtgaac ccgcgcggtt ggtcggcctc gcgatagctg agccgttcgc
      121 gctcgatcgc ctcgatctgg agggcgagga gctgcgagta gtagcgggcg gtctggatga
      181 tcccggtcat gaaccagagg ccgtcctggc cgcagcggcg ccagacgacg ctgagctcgc
      241 cgtcctcgcc cacctggtag atcgggcgga cgttgtcggc gagctcgccg agcagcggcc
      301 gcaccatgcc gaggatcttg ccgtagccgg tcgccatcac aatcacgtcg gcgtcgaggc
      361 tggagccgtc ggagagcacg gcggcggctg gggtgaggcg gtcgatggtc acgccggagc
      421 ggacgccgac gcggccgtcg gcgatcaggt ccgaggcgcc catgttgatc tggtagccgt
      481 cgcggccggc gatgtggagg acgaccacgc ccgcttcctc gggcccgtat tcgatcgcga
      541 acccctggcc ctggagcgcc tcgagcagct cggcgtcctg ctcccgggcc atgtccgtga
      601 ccattttccc gagcggcatg aactgtcccc aggggatcga ggcggcgatc aggtcggcgt
      661 cgagcagctc ctcggtcgag ccctcgaggt agttggcgaa gttgatgttg tggaaggttt
      721 cgccgttgat cacgtaggtc gcggagcgct gcagcagggt cacctcggcg cccgactcgt
      781 agaggtcctg gatcacgtcg tgcccgctca cccccgagcc gacgaccagg gccttcttgc
      841 ccttccaccg ggcgccgccc gggtagtcgc cggagtggac cagcgtgccc tcgaactcct
      901 ccgagccggc gatcgccggc aggttggggt tgccgttgag gccggtggcg atcacgaagt
      961 ggcgcggcgc cagcacgcgc tcggccccgt cctcgcgggt caccgtcagc gcccatcgct
     1021 gctcatcgcc gtcgaactcg gccgcgacca cgttcgaccc ggtccagacg ttgaggtcga
     1081 gggcgtcgac gtagtactcg aggaagttgg cgaacttgtc cttcggcgtg aacttcggcc
     1141 aggtatcggg gaagtggagg tagggcatgt ggtcggcgcc gaccgggtcg tgcagggcca
     1201 gcgaggggta tcggttgcgc cagttgtcgc cgacccgccg gttgcgctcg atcagcagcg
     1261 tcgggatccc gtgggcgccg agccgcgcgg cgagcgccag cccgccatgc ccggcgccga
     1321 ggatcaccac cgccggctgg gcttcggcga agtcgcgctc cgcctcgcgg gccgtcgccc
     1381 agtcgatccg gtccggggtg atcgtgtggt ccttgccgag cgggcgatgc gatccggact
     1441 gctccgggaa ccccgccagc tcctgcagct gggtcagcag atagaggcac ttccagccgc
     1501 cctccgcgtc gccgccgagg cgaacgaagc cctcgctcgg cccgcgctcg gtctcgaagc
     1561 ggatgaacag ggtcacctgg gagccgtctt cctcgaagcg gggctcacgg tcggtccgca
     1621 ccgtcagatc gccgaccggg accgctgcga gggcctcgag cagcgcggcc gcgacctcct
     1681 gcccgccggt ccagttcttg aagtcccact gaaagccgag caggtcccgc caggtgccgc
     1741 cctcggcaaa caggctgccg gcgcgctcga gatcaccctc ggcggcgctg gccaggagcg
     1801 cgtcgaccca tgactgcgca atctcggcgg cggacgaccg ccggctcgtg gactcgctcg
     1861 gctccatcac ttccctccgt tcccagtgac caattaacgc acaacctagc gcatttaacg
     1921 gttaagggat cagaattgtc gcgagccgct caccgctgca cgccctccag ctcgggcagc
     1981 cggaagcgcc gccggtaggc gcccggggtc agcctcgtgg tccgcttgaa gaggcggcgg
     2041 aaggcggccg ggtcgtcgta gccgaccgcc caggcgatct cctcgaccgg gtcggcgctg
     2101 gtctccagcc gccgcttcgc cttctcgact cggacctgct ggacgtaggc gatcggggtc
     2161 atctcggtcg ccgccttgaa gcggcgcttg aaggtggtcg gggcgagctc ggcgaccgcg
     2221 gccatctctt cgaccggggc ggcgaccgcg tagttggcgg cgatccagcg ctgggcggcg
     2281 agcaccgcgg cgtcctcgtg gtcggtcggc ggttcgaagc tggcgaaggc gtcctgcccg
     2341 tcgctgtgcc actgcacgag ctggaagcgg gcgagcgcct gggcggtggc cgggccgacg
     2401 tagcgggcga ccaggtagag cagcaggtcg tgccaggcgg tcgaggcgcc ggaggtgacc
     2461 aggcgcccgt cctcgccgga gacgaccagc gcctcctcgg gtcgcagtcg tacctccgga
     2521 tggcggcggc ggaacggggt cacggtgatc cagtgcgcgg tcgcctcctg gccgtcgagc
     2581 aggccggtct cggcggtgag catgccgccg gtgcaggccg agcagagcgt cgcgccgccc
     2641 tcgtacatgc ggcgcagcca ggcgaccagg tgcgggtggc ggccgacctc ccaggagtgg
     2701 tcttcggtca tcgccatcga ggggacgatc acgacgtcgg tgcggtcgac ctggtcgatc
     2761 gtcgcgtcgg gggcgatgct gaggccgctg ggcgagcgca ccggcccggc ccgctcggcg
     2821 acgattttca ccgcgaaagg ctcgccgccg gcacgctggg tttccggcga ctccagcccg
     2881 ccgaccgcgg ccagcgtctc gtagagaccg ctgacgggcg agaccatcgc ctccggcgtg
     2941 gcgacgatgc agacctcggt tggggctctc ttcgccccca ttcgaccccc gtggaagaaa
     3001 tggaccggtt gtgaggatta tgacccttgc ccagttgtcc gcctggatcg atcctggctc
     3061 cgaactttca gacgatccaa cgaacgaagg agcccttgtg aacccccaga cgatcgaact
     3121 cgacgaagcc cgcgtcgaag ccttcaacgc cgaggtgatc ggtcagctcg ccgcctgcta
     3181 cggcggcgtg atgatccggc tcggcgagca gctcggcctc tacgcggcgc tgcacggcgc
     3241 cggcccgctg acctcggccg agctggccgc gcgcagcggt tgcgccgagc gctacgtgcg
     3301 cgagtggctc ggcagccagg tcgcttccgg cgccctggac tacgaggccg gggcggagac
     3361 ctacgagctc tcgcccgagc acggcctcgt cctcgccgat cccgacagcc ccgcctacat
     3421 ggcctcggcc ttcggcgtcg cggccactct gtgggccgac gaggcgaagg cggttcgtgc
     3481 cttccgcacc ggcaccggcg tcccctgggg cgagcacgac gaacgcctct tctgcggcgt
     3541 cgcctccttc taccgcaacg cctacgcggc ctccctggtg ccggagtggc tgccggccct
     3601 ggaggggacg gtggcgaagc tcgaggcggg cgcccgggtc gccgacgtcg gttgcggcca
     3661 cggccactcg accgtgctga tggccgaggc cttcccggct tcgagcttcg tcgggatcga
     3721 cgtccacgcc gactcgatcg ctcacgctcg cgccaacgcc gaggccgccg gggtcgccga
     3781 ccgggtcgag ttcgtggtcg gcagcgcctc cgagtacggc cgcaacggct tcgacttgat
     3841 ctgcttcttc gatgccttcc acgacctcgg cgacccctac ggcgccgccg ccgaggcgcg
     3901 gcgggcgctc gccgcggacg gccaggtgat gctgatcgag ccgttcgccg ccgatggcgt
     3961 cgagggggcg ttggggccgg tcgcccagct ctactacgcg gcctcgacca cgctctgctg
     4021 cgcgcacgcg atctccgagg gtgccgagga cccgctcggc gcgcaggccg gggaggaccg
     4081 cctgcgcgcc gtcttcgagg aggccgggtt cggcgcctgg cgccgcgccg ccgagacccc
     4141 gttcaacctg gtgctcgaag cccgcgcctg aacagacgca cgcaggtggc gggtaccccg
     4201 atcggcttga tgtcgattgg gtcaacgacg cgacgccggt cttgcctgac ggttcccggc
     4261 tcgtcggagc ggatgctggc gaaagctccg gcgagcgggc cgaccagctg gtaatcgatc
     4321 tcgaggacgc ggtggcgttg gcggcatcgc aggccgctct gagcctttaa tccatcacta
     4381 ccgctttttt ctgtatggtt agccgccagg gaacttgaca aggaccgaga gggactttag
     4441 gttctgtacc gatcggtcgg aacgattggg cgaacgggaa ctcgcccgac gagaggggaa
     4501 agatggagaa ctcgcagctc agcagtcccg cgtcggaaca ggcgttcgtc ggcgtcgacg
     4561 tcggcggcac ccacaccgac gtccaggtga tcatgggcga gcgcgaagcg cgcggcaagg
     4621 cgctgacctc ttatgacgac ttcagccggg gcgtgctcgg cgccgtcggc gtcgccgccg
     4681 agaacctcgg gatcacgctc gaggagctgc tcagccgcac gcagctgctg gtcaacgcga
     4741 ccaccgtcgt caccaactcg atcacccagc tgaaaggcgc cgaggtcggg atcctcgtca
     4801 ccaacgggtt caaggacgag ttccgcttcg ccggcgggcc gcggatccgc gacgtcgacg
     4861 atcagatcca gaccaacgtc cccgacatcg tcaagcgccg caacgtgctc gaggtgcgtg
     4921 agcggatgca gttctccggc gaggcggccg tgccgctgaa cgaggacgac gtgctcagcc
     4981 aggcccgcct gctcgtcgac cgcggggtca gcgcgatcgc gatctgcttc ctcaacagct
     5041 actccaacgg cgcccacgag cagcgcgccg ccgagctgat cgaagacgag ttcccgcaga
     5101 tcttcgtcac cccctcgcac cgggtcagct cggttcgcgg cgagtacccg cgctggatga
     5161 ccgccgtgct caactgcttc gtccacgaac atgccaagcg tttcctggac gcgctcggga
     5221 ccaagctgca cgaggccggg ttgcgcggtc acgtcgcctt cttccagggc ctcggcggcg
     5281 gcgtcagcca gggccgcgcc gaacagctgc ccctgaccct gctcggcgcc ggcccggccg
     5341 gcggcgcgac cggctccaac gagcttgctc ggcgaatggg gcacgaccgc gtcctgctcg
     5401 gcgacatggg cggaaccagc ttcgacacgg gcctgatcca cgacaacgtc gtccacatcg
     5461 agaaggacat ccaggtcggc cgcttcaaga ccgcgatgcc gctggtcgac gtgatctcgg
     5521 tcggcgccgg cggcggctcg atcgcctgga tctccgagcg cggcgcgccc caggtggggc
     5581 cgcagagcgc cggctcctcg cccggtcccg ccgcctacaa caacggcggc accgaaccga
     5641 cggtcaccga cgcgatgatc gcgatggacc tgatcgatcc ggccaactac ctgggcggcc
     5701 gcgtcgaact gcgccgcgac ctcgcgatcg aggcgctgcg cactcgcttc gccgagccga
     5761 tggggtggag cgtcgaggaa ggtgcggcgg cggtccacga cctcgtcgtc gccaacatgg
     5821 ccaatgccgt ccgcgaggtc tcggtcgccc acggccacga cccccgcgag ttcgtcttct
     5881 acgcctacgg gggcaccctg ccctggttcg cggcgcagat cgccgaggtc ctgcagatcg
     5941 gcacggtgct ggtgccgcat tcgagctcgg tcttctgcgc ccgcggcctc ctggtctccg
     6001 acttcgtcct ccgaaacgac cagaccctgc agtcggggct ggccggcgag ggcgaggtcg
     6061 aacggatcaa cgaggcggcc cgctcgctgg ttgcggtcgg ccaggagagc atgcgcagcg
     6121 agggattcgg cgacgaggag atcgagatcg cccgctcggg cgacttccag ttcgccggcc
     6181 aggtgcacgc gctgccgatg ccgctgcccg accgcgagct ctccggcgac gacgtgccga
     6241 tgctgcagga tcagttcaac caggtttacg aacgcaccta cggcaagggc acggcctggc
     6301 ccggggtgcc gccgcagctg ctgaacctct cggtgacggt gaccggcaag ttgccgcggc
     6361 cgccgatcgc cgagcggccg ctcgacccgc gtgagccggc cgagatgctg cgcggcgagc
     6421 gcgaggtcta cctgccgacc gagcgcaaac gggagacgat cccgatctac gacgagacca
     6481 aggtcacgcc cggctcccgg gttgagggac cggccctggt cgaggctgtc g
//
LOCUS       DASRIG010000531         7381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_777433, whole genome shotgun sequence.
ACCESSION   DASRIG010000531 DASRIG010000000
VERSION     DASRIG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777433"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VFB52_14195"
     CDS             <1..1451
                     /locus_tag="VFB52_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZO07537.1"
                     /translation="FTTYGQAWNNGGPIAISWGWPLISIPILIIGFCLSELVSAYPTA
                     GGIYWWAGKLGGPVWAWFTGWFNLIGLAAVVASVDYACATFLNVILSLYEVNIFGINF
                     GDGSHVLQETFLLFFLILGAHTIVNIKRTHLLSTVNSVSVWWHVIGVAVIVGVLAFVP
                     DTHQSFDFVFTERINNSGFSDGMYWFYILPLGFLLTQYTITGFDASAHISEETHEAEM
                     AAAKGVWRSIFYSALIGWVVLLAITFAATDVDAVNEAAGFSPSIFESALGSGWTKLVM
                     IISTIGQLFCGAACLTSASRMCYAFSRDRGFGKRASAHISKVNELKVPRNAVLAMAAA
                     ALIITLPALEGTGEGVPWAFFAVVSVCVIGLYIAYVIPIYLRWRMGDAFEPGPWTNGK
                     KYKWMNSFATVWVIFITIIFCPPFTPFAVPWNDEFEIESLNYAPVTVGLVILLAAIAW
                     KVSAHKHFTGQVREIDLEKEIGEPPEVGPSAP"
     gene            1494..2822
                     /locus_tag="VFB52_14200"
     CDS             1494..2822
                     /locus_tag="VFB52_14200"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZO07538.1"
                     /translation="MTLDELREAVADGTVDTVLLAIADMEGRLQGKRLTASHFLDDVV
                     EHGAEGCNYLLAVDVEMETVGGYAMSSWDTGYGDFEMVPDLETLRPVPWLPGTAMLMA
                     DLQWADGRDVVASPRQILRRQLERLAERGMGANAATELEFIVFRDTYEEAWRKGFHEL
                     EPANLYNIDYSLLGTARVEPLIRRIRNEMSAAGMKVENSKGECNLGQHEINFRYADAL
                     RCADDHAIYKNGAKEIAAQEGHSITFMAKFNEREGNSCHIHCSLTDADGGNAFAADQK
                     LFEHYVAGQLACMRELTLLYAPHVNSYKRFAPGSFAPTAIAWGRDNRTCSLRVVGEGA
                     ALRVENRLPGADVNPYLALSALIAAGLHGIDNELPLEPVFEGDAYKSEKPRVPHNLYE
                     ARDAFGESEVVAEAFGQEVVEHYLNRAGVEIDAFEAAVTDWERYRGFERL"
     gene            2819..3541
                     /locus_tag="VFB52_14205"
     CDS             2819..3541
                     /locus_tag="VFB52_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZO07539.1"
                     /translation="MKPAIGICAAVERVSWGVWDGYEVTLVPRSYVSSVQRAGGIGIV
                     LPPDEAAVADPDLLLDRVDALIIAGGADIDPSSYGAAPHPETRGTWPDRDRFEIALAR
                     RALQRDMPLLGICRGMQLLNVALGGTLNQHLPDTIGASTHRSVAGTFSEHQVHLEQGS
                     LACAAAGTEGLMVLSHHHQGIDGLGEGLRVTGWSDEDELVEAIELPEKRYALGVVWHP
                     EEDAASPLIASLVEAARVGVAP"
     gene            3538..4899
                     /locus_tag="VFB52_14210"
     CDS             3538..4899
                     /locus_tag="VFB52_14210"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZO07540.1"
                     /translation="MIEVIEPASEEVLATLPRAGVDEVDEAVARAKAAFPAWRAVTPP
                     DRAALLRALADAIEGRAEELAQIEARNAGKPIGDARGEIGMVVETFRFYAGAPERLLG
                     QTIPVAGGVDMTFREPLGVVGLIVPWNFPLTIASWKVAPALAAGNTIVLKPAELTPLT
                     ALELEVLALDAGLPEGVLNVVAGPGSVCGQRLVEHPDVAKVAFTGSTAVGRGIAAGAA
                     ATIKRVTLELGGKSANVVFADADIEAAAAAAPGAVFGNAGQDCCARSRILVEQSAVDD
                     FLAALAPAVEAIAVGDPLDEATEMGPLISAGQRESVASFVPDDAPVAIRGSAPEGAGY
                     WFPPTVLAPVGNDDRAAREEIFGPVACVIPFADEAEAIRLANETIYGLSGSIWTRDGA
                     RALRCARAIESGVLSINSNTSVRVSTPFGGFKQSGVGRELGPDALDHYTELKNVYFAT
                     EEG"
     gene            4903..5661
                     /locus_tag="VFB52_14215"
     CDS             4903..5661
                     /locus_tag="VFB52_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07541.1"
                     /translation="MAGRLEGKVCVITGTASGIGAESARLFAEQGARVVGIDLDKAHA
                     EGETTIEADVSDEAQVAEAYQWIGTELGRIDVLMNNAGINPTDDSSVLETDLDAWQRV
                     QDVNLRSVFLCCKHGIPHLLAGGGGSVINVASFVATMGAAVSQISYTASKGAVLSLSR
                     ELGVEFANRGVRVNALCPGPVNTPLLKELFAKDPERAAKRLVHIPMARFGEPEEIAKA
                     ALFLASDDSSFVTASTFMVDGGLSAAYLTPGQVD"
     gene            5743..6681
                     /locus_tag="VFB52_14220"
     CDS             5743..6681
                     /locus_tag="VFB52_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZO07542.1"
                     /translation="MIGFALKVVLAVAVVVAVLVGVYFLLDEFDGDDPEPVEASGSGL
                     VIRAIGDSVTAGFGYADADGDRFDAIELPFCASRAAHPRCQDPDGVAYPARYASGVED
                     ANFVNYAISGSTAADWLGEGKRPELNARLQARFAKVVAADPDVTVLTLGANPLLDSFL
                     TDIAGRRLCATTRDRQKARACIRTALDEERVEARLQRIYDELLDTPDDGRNGLVVVFQ
                     YPESHPPSAIGVNVTVLVEELRLAIAASVARAIAADPEDGDRLVVAEPGAFIFHGCGD
                     DEPWILGVDTCIHPNVAGHAQLARVLADVVAEHEQP"
     gene            complement(6678..7160)
                     /locus_tag="VFB52_14225"
     CDS             complement(6678..7160)
                     /locus_tag="VFB52_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07543.1"
                     /translation="MSSPQLRAAAQEIVGDETVLAAGIFTIPDSIASLTKGSLAAAIA
                     IPDSVNPVLSGAGNVAALEMARAKEAESKGLTERMVVAVTESSIHLLALPHVGKTPER
                     ELFRLERDAFDVKISKLGLSRKLQLTHRESGEKLKLMGYTVGPTDVAKGDKAVLEALA
                     "
     gene            complement(7185..>7381)
                     /locus_tag="VFB52_14230"
     CDS             complement(7185..>7381)
                     /locus_tag="VFB52_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07544.1"
                     /translation="GGMALEALRIKVGTKPMLRVLKRWASGHAYGSANIKEFVVHAEK
                     VTGKNLDPLFQRWLYKRGKP"
BASE COUNT         1102 a         2552 c         2568 g         1159 t
ORIGIN      
        1 gtttcaccac ctacggccag gcctggaaca acggcggtcc gatcgcgatc agctggggct
       61 ggccgctgat ctcgatcccg attctgatca tcgggttctg cctctccgag ctggtctcgg
      121 cgtacccgac cgccggtggc atctactggt gggcgggcaa actcggtgga ccggtttggg
      181 cgtggttcac cggctggttc aacctgatcg gcctcgccgc cgtggtcgcc tcggtcgact
      241 acgcctgcgc gaccttcctc aacgtgatcc tcagcctcta cgaagtgaac atcttcggga
      301 tcaacttcgg tgacggctcg cacgtcttac aagagacctt cctgctcttc ttcctgattc
      361 tcggcgcgca cacgatcgtc aacatcaaac gaacgcacct cttatcgacg gtcaacagcg
      421 tctcggtctg gtggcacgtg atcggggtcg cggtgatcgt cggggtgctg gctttcgtgc
      481 cggacacgca ccagagcttt gacttcgtct tcaccgaaag gatcaacaac agcggtttca
      541 gcgacggcat gtactggttc tacatcctgc cgctcggatt cctgctgacc cagtacacga
      601 tcaccggctt cgacgcctcc gctcacatct ccgaggagac gcacgaagcc gagatggcgg
      661 cggccaaggg cgtttggagg tcgatcttct actcggcgct gatcggctgg gtcgtgctgc
      721 tggcgatcac cttcgcggcg accgacgtcg acgcggtcaa cgaagcggcg ggcttctcgc
      781 cgtcgatctt cgaatcggcg ctgggctccg gctggaccaa gctggtgatg atcatctcca
      841 cgatcggcca gctgttctgc ggcgccgcct gcctgaccag cgcctcgcgc atgtgttacg
      901 ccttctcccg cgatcgcggg ttcggcaagc gcgcctcggc ccacatctcc aaggtcaacg
      961 agctgaaagt gccgcgcaac gcggtgctgg cgatggcggc ggcggcgctg atcatcaccc
     1021 tgccggcgct cgagggcacc ggcgaaggcg tgccgtgggc cttcttcgcg gttgtctccg
     1081 tctgcgtgat cgggctctac atcgcctacg tgatcccgat ctacctgcgc tggcggatgg
     1141 gcgacgcgtt cgaaccgggt ccctggacga acggcaagaa gtacaagtgg atgaactcgt
     1201 tcgcgaccgt ctgggtcatc ttcatcacga tcatcttctg cccgccgttc acgccgttcg
     1261 cggtgccctg gaacgacgag ttcgaaatcg agtcgttgaa ctacgcgccg gtcacggtcg
     1321 gtctcgtgat cttgctcgcc gcgatcgctt ggaaggtgag tgcgcacaag cacttcaccg
     1381 gacaggtgag agagatcgat ctggagaaag agatcggcga gccgcccgaa gtaggaccgt
     1441 cggccccatg agggcccggg tgcagacgtt cgcgggcgcg taacgttccc ggcgtgaccc
     1501 tcgacgagct cagagaggcg gtcgcggatg gcacggtcga caccgtgctg ctcgcgatcg
     1561 ccgacatgga ggggcggctg cagggcaagc ggctgaccgc ctcgcacttc ctcgacgacg
     1621 tggtcgagca cggcgccgag ggctgcaact acctgctcgc ggtggacgtc gagatggaga
     1681 ctgtcggcgg ctacgcgatg tcctcctggg acaccggcta cggcgacttc gagatggtcc
     1741 ccgacctgga gacgctgcgg ccggttccct ggctgccggg gacggcgatg ctgatggccg
     1801 acctgcagtg ggccgacggc cgcgacgtgg ttgcctcgcc gcggcagatc ctgcgtcgtc
     1861 agctcgagcg tctggccgag cgcgggatgg gcgccaacgc cgccaccgag ctcgagttca
     1921 tcgtcttccg cgacacctac gaggaggcct ggcgcaaggg cttccacgag ctcgagccgg
     1981 ccaacctcta caacatcgac tactcgctgc tcggcaccgc gcgggtcgag ccgctgatcc
     2041 ggcggatccg caacgagatg tcggcggcgg ggatgaaggt ggagaactcg aagggcgagt
     2101 gcaacctcgg ccagcacgag atcaacttcc gctacgccga cgcgctgcgc tgcgccgacg
     2161 accacgcgat ctacaagaac ggcgccaagg agatcgccgc ccaagagggg cactcgatca
     2221 ccttcatggc gaagttcaac gagcgcgagg gcaactcgtg ccacatccac tgctcgctga
     2281 ccgacgccga cggcggcaac gcgttcgccg ccgaccagaa gctgttcgag cactacgtcg
     2341 ccgggcagct cgcctgcatg cgcgagctga ccctcctcta cgccccccac gtcaactcct
     2401 acaaacggtt cgcgcccggc tcgttcgcgc cgaccgcgat cgcctggggc cgggacaacc
     2461 gcacctgctc gctgcgggtg gtcggcgagg gcgcggcgct gcgggtcgag aaccgcctgc
     2521 ccggcgccga cgtcaacccg tacctggcgc tgagcgcgct gatcgccgcc ggcctgcacg
     2581 ggatcgacaa cgagctgccg ctggagccgg tcttcgaggg cgacgcctac aaatcggaaa
     2641 agccccgcgt tccccacaac ctctacgaag cccgcgacgc cttcggcgag agcgaggtgg
     2701 tcgcggaggc cttcggccag gaggtggtcg agcactacct gaatcgggcc ggcgtcgaga
     2761 tcgacgcctt cgaggccgcg gtcaccgact gggagcgcta ccggggcttc gagcggctgt
     2821 gaagcccgcg atcgggatct gcgccgcggt cgagcgcgtc tcctgggggg tctgggacgg
     2881 ctacgaagtg acgctggtgc cgcgcagcta cgtgagctcg gtgcagcggg ccggggggat
     2941 cgggatcgtg ctgccacccg atgaggcggc ggtcgccgat ccggacctgc tgctcgaccg
     3001 ggtcgacgcg ctgatcatcg ccggcggcgc cgacatcgac ccgtccagct acggggccgc
     3061 gccgcatccc gagacgcgcg ggacctggcc cgaccgcgac cgcttcgaga tcgccctcgc
     3121 gcggcgggcg ctgcagcgcg acatgcctct gctcgggatc tgccgcggga tgcagctgct
     3181 gaacgtggcg ctcggcggca cgctgaacca gcacctgccg gacacgatcg gggcctcgac
     3241 ccaccgtagc gtcgccggca ccttcagcga gcaccaggtg cacctcgagc agggctcgct
     3301 cgcctgcgcc gccgccggca ccgaggggct gatggtcctc tcccaccacc accaggggat
     3361 cgacgggctc ggcgaggggc tgcgggtgac cggctggtcc gacgaggacg agctggtcga
     3421 ggcgatcgag ctgccggaga agcgctacgc gctcggcgtc gtctggcacc ccgaggagga
     3481 cgcggcgagc ccgctgatcg cctcgctggt cgaagcggcg cgggtggggg tggcgccgtg
     3541 atcgaggtga tcgagccggc cagcgaggag gtgctggcga cgctgccgcg ggccggggtc
     3601 gatgaggtcg acgaggcggt ggcgcgggcg aaggccgcgt tcccggcctg gcgcgcggtc
     3661 acgccgccgg accgggcggc gctgctgcgg gcgctcgccg acgcgatcga ggggcgcgcc
     3721 gaggagctgg cgcagatcga ggcgcgcaac gccggcaagc cgatcggcga cgcgcgcggc
     3781 gagatcggga tggtggtcga aaccttccgc ttctacgcgg gcgcaccgga gcggctgctc
     3841 gggcagacga tcccggtcgc cggcggcgtc gacatgacct tccgcgagcc gctcggggtc
     3901 gtcgggctga tcgtgccctg gaacttcccg ctgacgatcg cctcctggaa ggtcgcgccg
     3961 gcgctcgccg ccggcaacac gatcgtcctg aagccggccg agctgacgcc gctgaccgcg
     4021 ctcgagctgg aggtgctggc gctcgacgct ggcctccccg agggcgtgct gaacgtcgtc
     4081 gccgggcctg gcagcgtctg cggtcagcgc ctggtcgagc accccgacgt cgccaaggtc
     4141 gccttcaccg gctcgaccgc cgttggtcgc gggatcgccg ctggggcggc ggcgacgatc
     4201 aagcgggtga ccctggagct gggcggcaag tcggccaacg tcgtcttcgc cgatgccgac
     4261 atcgaggccg ccgccgcggc ggctcccggg gcggtcttcg gcaacgccgg ccaggactgc
     4321 tgcgcgcgct cgcggatcct ggtcgagcag agcgcggtcg acgacttcct cgcggcactg
     4381 gcgccggcgg tcgaggcgat cgcggtcggc gacccgctcg acgaggcgac cgagatggga
     4441 ccgctgatct ccgccggcca gcgcgagtcg gtcgcctcct tcgtccccga cgacgccccg
     4501 gtggcgatcc gcggcagcgc ccccgagggg gccggctact ggttcccgcc gaccgtgctg
     4561 gcgccggtcg gcaacgacga ccgcgccgcc cgcgaagaga tcttcggccc ggtcgcctgc
     4621 gtgatcccgt tcgccgacga ggcggaggca atccggctcg ccaacgagac gatctacggc
     4681 ctctccggct cgatctggac gcgcgacggg gcgcgggccc tgcgctgcgc gcgggcgatc
     4741 gagagcggcg tgctctcgat caactcgaac acgtcggtgc gcgtctcgac gccgttcggc
     4801 gggttcaagc agtccggcgt cggccgtgag ctcggccccg acgcgctcga ccactacacc
     4861 gagctgaaga acgtctactt cgcgaccgag gagggttgag tcttggctgg acgacttgag
     4921 ggcaaggtct gcgtgatcac cggcaccgcg agcgggatcg gcgccgagtc ggcgcggctg
     4981 ttcgccgagc agggcgcgcg cgtggtcggg atcgacctcg acaaggcgca cgccgagggc
     5041 gaaacgacga tcgaggccga cgtctccgac gaggcccagg tcgccgaggc ctatcagtgg
     5101 atcggcaccg aactcggtcg catcgacgtg ttgatgaaca acgccgggat caacccgacc
     5161 gacgacagct cggtgctgga gaccgacctc gacgcctggc agcgcgtcca ggacgtcaac
     5221 ctgcgcagcg tcttcctctg ctgcaagcac ggcatcccgc acctgctcgc gggcggcggc
     5281 ggctcggtga tcaacgtcgc ctcgttcgtc gcgacgatgg gcgccgccgt ctcgcagatc
     5341 tcctacacgg cctcgaaggg cgcggtgctc tcgctctcgc gtgagctcgg ggtcgagttc
     5401 gccaaccgcg gcgtgcgcgt caacgcgctc tgcccgggcc cggtcaacac gccgctgctc
     5461 aaggagctgt tcgcgaagga tcccgagcgc gccgccaagc gcctcgtcca catcccgatg
     5521 gcccgcttcg gcgagcccga ggagatcgcc aaggcggcgc tcttcctcgc cagcgacgac
     5581 tcctcgttcg tcaccgccag caccttcatg gtcgacggcg gcctctccgc cgcctatctg
     5641 acgcccggcc aggtcgacta gtcctacagc ggggtgcgca gcgggggttt accctgctcg
     5701 ctcacgccca ctgagtaagc gggggaagta gatggtgcgg gagtgatcgg gtttgcgctc
     5761 aaggtcgtgc tcgcggtggc ggttgtcgtc gccgtgctcg tcggtgtcta cttcctgctc
     5821 gacgagttcg acggcgacga ccccgaaccg gtcgaggcga gcggcagcgg gctggtgatc
     5881 cgggcgatcg gcgactcggt gaccgccggg ttcggctacg ccgacgcgga cggcgaccgg
     5941 ttcgacgcca tcgaactgcc gttctgcgcc tcgcgcgctg cccatccgcg ctgccaggac
     6001 cccgacggcg tcgcctatcc ggcgcgctac gcgagtggcg tcgaggacgc caacttcgtc
     6061 aactacgcga tctcgggatc gaccgcggcc gactggctcg gcgaaggaaa gaggccggag
     6121 ctgaacgcgc gcctgcaggc gcgcttcgcc aaagtggtgg cggccgaccc cgacgtcacc
     6181 gtgctcacgc tcggcgccaa cccgctgctg gacagcttcc tcaccgacat cgccggccgc
     6241 cgcctctgcg cgacgacccg cgaccgccag aaagcccgcg cctgtatccg caccgcgctc
     6301 gacgaagagc gggtggaagc acggctgcag cggatctacg acgagctgct cgacaccccg
     6361 gacgacggca ggaacgggct ggtcgtcgtc ttccagtacc ccgagagcca cccgcccagc
     6421 gcgatcggcg tcaacgtcac cgtcctggtc gaagagctgc ggttggcgat cgcagcttcc
     6481 gtcgcccggg cgatcgccgc cgaccccgag gacggtgacc ggctggtggt cgccgagccg
     6541 ggcgcgttca tcttccacgg ctgcggcgac gacgagccgt ggatcctcgg cgtcgacacc
     6601 tgcatccatc ccaacgtcgc cggtcacgcc cagctcgcgc gggtgctcgc cgacgtcgtc
     6661 gccgagcacg agcagcccta ggcgagcgct tccagcaccg ccttgtcgcc cttggcgacg
     6721 tcggtcggcc cgaccgtgta gcccatcagc ttcagcttct cgcccgactc gcggtgggtg
     6781 agctgcagtt tgcggctgag gccgagcttc gagatcttca cgtcgaaggc gtcgcgctcg
     6841 agccggaaga gctcccgctc gggcgtcttg ccgacgtgcg gcagggcgag caggtggatc
     6901 gagctctcgg tcacggcgac gaccatgcgc tcggtcagcc ctttcgactc ggcctccttg
     6961 gcgcgggcca tttcgagggc ggcgacgttg ccggcgccgg agaggaccgg gttgacgctg
     7021 tcgggaatcg cgatcgcggc ggcgaggctg cccttggtca gcgaggcgat cgagtcgggg
     7081 atcgtgaaga tgccggcggc gaggaccgtt tcgtcgccga cgatctcctg ggccgcggcg
     7141 cgaagctggg gagaagacat gcgagcactc cttctttgag tgacctacgg ctttccgcgc
     7201 ttgtagagcc agcgctggaa cagcgggtcg aggttcttac cggtcacctt ctcggcatga
     7261 acgacgaact ccttgatgtt ggcgctgccg taggcgtggc cactggccca acgtttgagc
     7321 acgcgcagca tcggcttcgt cccgaccttg atccgcagcg cctccagcgc catcccgccg
     7381 c
//
LOCUS       DASRIG010000532         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_777640, whole genome shotgun sequence.
ACCESSION   DASRIG010000532 DASRIG010000000
VERSION     DASRIG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777640"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFB52_14235"
     CDS             complement(<1..274)
                     /locus_tag="VFB52_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07545.1"
                     /translation="MLLAALAVAGPASAATKPAAKDKYANYPPPELGPGHQAGPKPHL
                     MLLHGGSFLYDDPEFVASTKLAAEKAGFIPHYTVYPLGDMPAAVLAV"
     gene            complement(367..>2124)
                     /locus_tag="VFB52_14240"
     CDS             complement(367..>2124)
                     /locus_tag="VFB52_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HZO07546.1"
                     /translation="YWGTPLPIWRNEAGEALAVGSFEELQQLSGVALEDPHRPYVDDM
                     TYDCADCDSPMRRVPEVIDVWFDSGAMPFAQFHYPFENKDLFEERFPADYICEAQDQT
                     RGWFYTLLAESTLLFGESSFRNCVCLGLILDPEGQKMSKSRGNVVDPWEVLSTHGADA
                     FRWYYLATQQPWAGYRFSVETVGEAVRQFLLTLWNTYSFWVLYANAENLGPLDFHGTP
                     EPATDLDRWALSRLQATVATVRERMDDFDCTAAGKAIAEYVEELSNWYVRLSRRRFWE
                     GDRAAFATLRHCLLETVALLAPFTPFLADEIHLNLAGGTLEETGELFDSVHLRDFPEV
                     DPALADPELEEAMVAVRLTVELGRAARAQAKAKVRQPLRRAVIVANDAERAAIEARAD
                     LVTAELNVKELDFVTDQSELVSYTAKPNYRALGPRFGKDMPQVAAAVEALDAAHVAEV
                     LAAGGEVGISIGGSDHSLAPEDLTLALQPLEGYEVEAEAGHAVALQLELDEELRREGL
                     AREIVHAVQNARKEAGLEITDRIALGLGGEQALLEAAREHQAYLAGEVLANEIAFEGG
                     NGTTVTIDGMELAIAVERT"
BASE COUNT          306 a          709 c          782 g          327 t
ORIGIN      
        1 gcaccgccag caccgccgcc ggcatgtcgc cgagcgggta gaccgtgtag tgggggatga
       61 agccggcctt ttcggcggcg agcttggtcg aggcgacgaa ttcgggatcg tcgtagagga
      121 aggagccgcc gtgcaggagc atcaggtgcg gcttcgggcc ggcctggtgg ccggggccga
      181 gttcgggcgg cgggtagttg gcgtatttgt ctttggccgc gggtttggtc gcagcggaag
      241 ccggcccggc gacggccagg gcggcgagca gcacgaggaa gactgcgagg ctggggatcg
      301 cgcatctacg ggggatacgt gcaaaacccc gctgaaggtc cgttgtcgcg ccccgcaacg
      361 cgatcctcaa gtccgctcca cggcgatcgc cagctccatg ccgtcgatcg tcacggttgt
      421 gccgttgccg ccctcgaagg cgatctcgtt ggccagaacc tcgccggcca gataggcctg
      481 gtgctcgcgt gcggcttcga gcagggcctg ctcgccgccg agcccgagcg cgatccggtc
      541 ggtgatctcc aggccggcct ctttgcgcgc gttctggacc gcgtgcacga tctcgcgcgc
      601 cagcccctcg cggcgcagct cctcgtcgag ctccagctgc agggcgaccg cgtggcccgc
      661 ctcggcctcg acctcgtagc cctcgagcgg ctgcagggcg agggtgaggt cctcgggcgc
      721 gaggctgtgg tcgctgccgc cgatcgagat cccgacctct cccccggccg ccagcacttc
      781 ggcgacgtgg gcggcatcga gcgcctccac cgcagcggcg acctgcggca tgtccttgcc
      841 gaagcgcgga ccgagcgccc ggtaattcgg ctttgcggtg taactaacca gctcgctttg
      901 atcggtgacg aagtcgagct ccttgacgtt gagctcggcg gtgaccaggt cggcgcgcgc
      961 ttcgatcgcg gcgcgctcgg cgtcgttggc gacgatcacg gcacggcgca gcggctggcg
     1021 caccttcgcc ttcgcctggg cgcgggcggc gcggccaagc tcgacggtca gccgcacggc
     1081 caccatcgcc tcctccagct ccgggtcggc gagcgccggg tcgacctccg ggaagtcgcg
     1141 caggtggacg gagtcgaaca gctcccctgt ctcctccagc gtgcccccgg cgaggttgag
     1201 gtggatctcg tcggcgagga agggcgtgaa cggcgccagc agcgcgaccg tctccagcag
     1261 gcagtggcgc agggtggcga aggcggcgcg gtcgccttcc cagaagcgcc ggcgcgagag
     1321 ccgcacgtac cagttggaga gctcctcgac gtactcggcg atcgccttgc cggcggcagt
     1381 gcagtcgaag tcgtccatgc gctcgcgcac ggtcgcgacc gtcgcctgca gccgcgagag
     1441 cgcccagcgg tcgaggtcgg tggcgggctc gggcgtgccg tggaagtcga gcggtccgag
     1501 gttctcggcg ttcgcgtaga ggacccagaa cgagtacgtg ttccagagcg tgagcaggaa
     1561 ctggcgcacc gcctcgccga ccgtctcgac cgagaagcgg tagccggccc agggctgctg
     1621 ggtcgccagg tagtaccagc ggaaggcgtc ggcgccgtgg gtggaaagca cctcccaggg
     1681 gtcgacgacg ttgccgcggc tcttcgacat cttctggccc tcggggtcga ggatcaggcc
     1741 gaggcagacg cagttgcgga agctcgactc gccgaacagc agggtcgact cggcgagcag
     1801 cgtgtagaac cagccgcggg tctggtcctg ggcctcgcag atgtagtcgg ccgggaaccg
     1861 ttcctcgaac aggtccttgt tctcgaacgg gtagtggaac tgcgcgaacg gcatcgcgcc
     1921 cgagtcgaac cagacgtcga tcacctcggg cacgcggcgc atcggcgagt cgcagtccgc
     1981 gcagtcgtag gtcatgtcgt cgacgtaggg ccggtgcggg tcctccagcg cgacgccgga
     2041 gagctgttgc agctcctcga aggagccgac ggccagcgcc tcgcccgcct cgttgcgcca
     2101 gatcggcagc ggcgtgcccc agta
//
LOCUS       DASRIG010000533         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_777721, whole genome shotgun sequence.
ACCESSION   DASRIG010000533 DASRIG010000000
VERSION     DASRIG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777721"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..832
                     /locus_tag="VFB52_14245"
     CDS             83..832
                     /locus_tag="VFB52_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZO07547.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGKLFSKLARAITVAARDGGGDTE
                     GNPALATAVQKAREASMPKDKIQKAIDTGTGAGSDSAAIERVLYEGYGPSGVAVLVEA
                     LTDNRNRASAEIRHAFSSHGGSLGEPGSVAWIFEKKGAVAVDAERYTEDDLMAAIDAG
                     AEDVREDDDRLTVLCEPSDLTAVKEALEGEGVEIVSATIVTEPKSTVELKGHDAERLL
                     KLLDTLDDQDDVDEVFANFDISEDVLEKLAA"
     gene            878..1402
                     /gene="ruvC"
                     /locus_tag="VFB52_14250"
     CDS             878..1402
                     /gene="ruvC"
                     /locus_tag="VFB52_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZO07548.1"
                     /translation="MQVVMGVDPGAANLGFGIVRVEGNHMVALDGGVVETTPDLPMER
                     RLERIHSALAELIAWHEPKALAIEEVYFGKNVRSAMAVGQASGVAMLAAAERGVSCFA
                     YTPQAIKMAVCGSGAADKRQVQRMVGTLLGLPEPPSPDHAADALAVAICHGGGNGHRQ
                     AVASARPARAGQVA"
     gene            1402..1995
                     /gene="ruvA"
                     /locus_tag="VFB52_14255"
     CDS             1402..1995
                     /gene="ruvA"
                     /locus_tag="VFB52_14255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZO07549.1"
                     /translation="MIAAVRGEVMVRRPDHVVIDAAGVGYRLAVSSETLKAVPAAGSD
                     AFLHAELIAREDSLALYGFATEEERELFRLLISVSGIGPKVAIATLSGGSSRDLLSAI
                     AAGDAKRFQAVPGIGKRTAERIIVELREKVSGTLEEEVALAAVEGGDARALARDGLVN
                     LGYAPLEAEQLLDGLSGEDPETLLASALRKAGTGRAA"
BASE COUNT          337 a          686 c          716 g          280 t
ORIGIN      
        1 ctgccacgct gcgggccgca ctgcggggtg cggcgtgcgt gtgtgcgttc gagttcatct
       61 cccacttcat agaatgacag gcatgtccgg tcactcaaag tggtcttcca ttaagcacaa
      121 gaagggcgcc gccgacgcca aacggggcaa gctcttcagc aagctcgcgc gcgcgatcac
      181 cgttgccgcc cgcgatggcg gcggcgacac cgagggcaac ccggcgcttg ccaccgcggt
      241 tcagaaggcg cgcgaagcgt cgatgccgaa agacaaaatc cagaaagcga tcgacaccgg
      301 caccggggcc ggctccgaca gcgcggcgat cgaacgggtt ctctacgagg gctacggccc
      361 cagcggggtc gcggtcctgg tcgaggcgct gaccgacaat cgcaaccgcg ccagcgccga
      421 gatccggcac gcgttctcca gtcacggcgg cagcctcggc gagcccggct cggtcgcctg
      481 gatcttcgag aaaaagggtg cggtcgcggt cgatgccgag cgctacaccg aggacgactt
      541 gatggccgca atcgatgccg gcgcagagga cgtgcgcgag gacgacgacc gcctcaccgt
      601 gctctgcgag ccgagcgacc tgaccgcggt caaagaggcg ctcgaaggag agggcgtgga
      661 gatcgtctcg gcgacgatcg tgaccgagcc gaaaagcacg gtcgagctga aaggtcacga
      721 cgccgagcgg ctgctgaagc tgctcgacac gctcgatgat caggacgacg tggacgaagt
      781 tttcgccaac ttcgacatct ccgaggacgt gttggagaaa ttggccgcct gagcggcccg
      841 cggaaggcat ccggccggct ccccagaatc actccccatg caggtagtga tgggggttga
      901 ccccggcgca gcaaacctcg gattcggcat cgtccgcgtc gagggcaacc acatggtcgc
      961 cctcgacggc ggcgtggtcg agaccacgcc ggatttgccg atggagcgcc gtctggagcg
     1021 gatccacagc gccctcgccg agctgatcgc ctggcacgag ccgaaggcgc tggcgatcga
     1081 ggaggtctac ttcggcaaga acgtgcgctc ggcgatggcc gtcggccagg cttcgggcgt
     1141 ggcgatgctc gctgcggctg agcgcggcgt cagctgtttc gcctacacgc cgcaggcgat
     1201 caagatggcg gtctgcggct ccggcgccgc cgacaagcgc caggtccagc gcatggtcgg
     1261 caccttgctc gggctgcccg agccgccgag tcccgaccac gcggccgacg ccctcgcggt
     1321 ggcgatctgc cacggcggtg gcaacggcca ccggcaggcg gtcgcgtcgg cacgaccggc
     1381 gcgcgcgggg caagtggcct gatgatcgcg gccgttcgcg gtgaggtgat ggtgcggcgt
     1441 cccgaccacg tcgtgatcga cgccgccggc gttggctacc ggctcgcggt ctcctcggag
     1501 acgctgaaag cggtccccgc cgccggcagc gacgctttcc tgcacgccga gctgatcgcc
     1561 cgcgaagact cgcttgccct ctacggcttc gccaccgagg aggagcgcga gctcttccgg
     1621 ctgctgatct cggtcagcgg gatcggcccg aaagtcgcga tcgcgaccct ttccggcggc
     1681 agctcccgcg acctgctcag cgcgatcgcc gccggcgacg caaaacgctt ccaggcggtg
     1741 ccggggatcg gcaagcggac cgccgagcgg atcatcgtcg agctgcgcga gaaagtctcc
     1801 ggcacgctgg aggaagaggt ggcgctggcc gcggtcgagg gcggcgacgc ccgcgcgctc
     1861 gcccgcgacg gcctcgtcaa cctcggctac gcgccgctcg aagccgagca gctgctcgac
     1921 ggtctctccg gtgaggatcc cgagaccctg ttggccagcg ccctgcgcaa agccggcacc
     1981 gggagggccg cgtgagcgac ggccccgaga tcggcggcg
//
LOCUS       DASRIG010000534         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_777846, whole genome shotgun sequence.
ACCESSION   DASRIG010000534 DASRIG010000000
VERSION     DASRIG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777846"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VFB52_14260"
     CDS             <1..1821
                     /locus_tag="VFB52_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZO07550.1"
                     /translation="EFTGRILEGRRWSEGLHQAVEAKEGVAIREENQTLATITLQNYF
                     RLYDKLSGMTGTALTEATEFMKIYKIPVVEIPTNQPMVRDDHNDQIFKTKDGKWKAVL
                     DEIVGRHERGQPILVGTVSVETSEMISEALKRDGIKHAVLNAKPEHAEREGELIAQAG
                     RKGEVMIATNMAGRGVDIKLGGDAEHLAIHELKKQGITHESEDYQQALAAKTEELKPQ
                     CQAEAEEVRELGGLFICGTERHESRRIDNQLRGRAGRQGDPGESRFFLSAEDDVIRLF
                     AGDRIYKILDKLGPVDEDGTEVPLEAKMLTKTVENAQKKVEEQNFLIRKRVLEYDDVM
                     NEQRRVVYKYRREILEGRDMSETARDEVEGVVDRLVDEYTSAELLEDWDLEELETQLR
                     QIWPVSIEVGSLAPEQVDREKLKDALDDDAMSAYDEREEQLGDELMRYLERSILLQVI
                     DNRWREHLFDMDYLREGIHLRGFAQIDPLVAYKNEGFTMFEELMHSIWDEFSRLVFRA
                     QIEVQPAGGAVAAGDGEPTALDYSGPAEGQPSALQQMRTTGGAAAAAGAVDGAASQAV
                     SGNGGGEVVETVVRDEHDKVGRNDPCWCGSGKKYKKCHGA"
     gene            1840..2226
                     /locus_tag="VFB52_14265"
     CDS             1840..2226
                     /locus_tag="VFB52_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07551.1"
                     /translation="MPDPVREFIRAFNERDLDGFEATLDPEVELHSMKGLRKGIEAAR
                     QWATRAPGGTQQEIELEELYEDGTEAGGGRAVALIVRRWHWEENGEMAAEEPMAWLFE
                     LREHRVLSWRPYEDRAEALRAGGFSH"
BASE COUNT          477 a          778 c          807 g          301 t
ORIGIN      
        1 gagttcaccg gccgcatcct cgagggcagg cgctggtccg aaggcctcca ccaggcagtc
       61 gaggcgaagg aaggcgtggc gatccgcgag gagaaccaga cgctggcgac gatcaccctg
      121 cagaactact tccgcctcta cgacaagctc tccgggatga ccggtaccgc cctgaccgag
      181 gcgaccgagt tcatgaagat ctacaaaatc ccggtggtcg agatcccgac caaccagccg
      241 atggtccgcg acgaccacaa cgaccagatc ttcaaaacca aagacggcaa gtggaaagcg
      301 gtgctcgacg aaatcgtcgg ccgccacgaa cgaggtcagc cgatcctggt cggaaccgtc
      361 tcggtcgaga cctcggagat gatctccgag gcgctcaaac gcgacgggat caaacacgcg
      421 gtcctcaacg cgaagcccga gcacgccgaa cgagagggcg agctgatcgc ccaggcgggg
      481 cgcaagggcg aggtgatgat cgcgaccaac atggccggcc gcggcgtcga catcaagctt
      541 ggcggcgacg ccgaacacct ggcgatccac gagctgaaaa agcagggaat cacgcacgag
      601 agcgaggatt accagcaggc gctggccgcg aagaccgagg agctgaaacc gcagtgccag
      661 gccgaggccg aggaagtccg cgagctcggc ggtctgttca tctgcggcac cgagcgccac
      721 gagtcccgcc gcatcgacaa ccagctgcgc ggccgcgccg gccgccaggg cgaccccggt
      781 gagtcgcgct tcttcctctc cgccgaagac gacgtgatcc ggctcttcgc cggcgatcgc
      841 atctacaaaa tcctcgacaa actcgggccg gtcgacgagg acgggaccga agtcccgctc
      901 gaggcgaaga tgctgacgaa aaccgtcgag aacgcgcaga agaaagtcga ggagcagaac
      961 ttcctgatcc gcaaacgcgt gctcgagtac gacgacgtga tgaacgagca gcggcgcgtg
     1021 gtctacaaat accgccgcga gatcctcgaa ggccgcgaca tgtcggagac cgcccgcgac
     1081 gaggtcgagg gcgtcgtcga ccggctggtc gacgagtaca cctcggccga gctgctcgag
     1141 gactgggacc tcgaagagct ggagacgcag ctgcgccaga tctggccggt aagcatcgag
     1201 gtcggttcgc tcgcgcccga gcaggtcgat cgcgaaaaac tcaaagacgc gctcgacgac
     1261 gacgcgatga gcgcctacga cgagcgcgaa gagcagctcg gcgacgagct gatgcgctac
     1321 ctggagcgct cgatcctgct acaggtgatc gacaaccgct ggcgcgaaca cctcttcgac
     1381 atggactacc tgcgcgaagg catccacctc cgcggcttcg cccagatcga cccgctggtc
     1441 gcttacaaaa acgagggctt cacgatgttc gaggagctca tgcactcgat ctgggacgag
     1501 ttcagccgcc ttgtcttccg tgcccagatc gaagtccagc cggccggcgg cgccgtcgcc
     1561 gccggcgatg gcgagccgac cgcgctcgac tactccgggc cggccgaggg gcagccctcg
     1621 gcgctccagc agatgcgaac caccggtggc gccgccgcgg ccgccggcgc cgtcgacggt
     1681 gccgcctcgc aggcggtcag tggcaacggc ggtggcgagg tggtcgagac cgtggtccgc
     1741 gacgagcacg acaaagtcgg ccgcaacgac ccctgctggt gtggcagcgg caagaagtac
     1801 aagaagtgcc acggcgcctg agcgccgcac ctgccgaaga tgcccgatcc cgtccgcgag
     1861 ttcatccggg cgttcaacga gcgtgacctc gacgggttcg aggcgacgct tgaccccgag
     1921 gtcgagctgc actcgatgaa ggggctgcgc aaggggatcg aggcggcgcg ccagtgggca
     1981 acgcgggcgc cgggcgggac ccagcaggag atcgagctgg aggagctcta cgaggacggc
     2041 accgaagccg gcggcggccg cgcggtggcg ctgatcgtgc gccgctggca ctgggaggag
     2101 aacggcgaga tggccgcgga ggaaccgatg gcctggctgt tcgagctgcg cgagcaccgc
     2161 gtactcagct ggcgccccta cgaggaccgc gccgaggcgc tgcgggccgg gggcttctcc
     2221 cactagtctt cagcgatggc cgatgccgcg cagaccgaag ccgtagcgcc gcgcctcgcc
     2281 gctgtccgcg agcagctgtc cctgctctcg gactaccttt gacccggctg ggctgaaagc
     2341 cgaggctgac cgcctcgaag cgg
//
LOCUS       DASRIG010000535         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_778373, whole genome shotgun sequence.
ACCESSION   DASRIG010000535 DASRIG010000000
VERSION     DASRIG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778373"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFB52_14270"
     CDS             <1..874
                     /locus_tag="VFB52_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZO07552.1"
                     /translation="IPTSCGPPCTIVQTQMPGPAFAASPVTGTVIRWRMQGGSPSYPY
                     RLRVVSRSGSTYTATGSSPSVVPLGPAAQAFPVSIPIQAGQLIGIDLQPSAPLDRREF
                     VPGAAYAYITPPVADGVPTPGVEAPEFPYEWAINADVLPPPSIASIAPAQGILASGST
                     VKVKITGTNFAEVTGVTFGGVPTTYTVDSDSQITALPAAQKKPGPQAVTVTTLAGTAT
                     APKPYRYTACVVPKLAGKKLKAAKRLIRKGECKVGFVKKRGEATAKTGRVVAQKPPAG
                     RTVRTGSKVRVTLG"
     gene            915..1892
                     /locus_tag="VFB52_14275"
     CDS             915..1892
                     /locus_tag="VFB52_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZO07553.1"
                     /translation="MLLAVVVGAIVFAVPRPADAGIVTVGNPLDKGSAFSIASFAGST
                     FVNGTRTGPGALAASPVDGVILRWRTRGNYIGGPFRLRVVRPVGSGFVGAGTSAPVTP
                     LGEALQAFPTQLPIRAGDLIGIDTSAKTDFVATTDPVAGTDMTIISPPLVDGGPTQTG
                     TTSHNFYELMINADVQPAPKITQLGPLDGSVKGGTEVNVIGTDFTRVSGVSFGSLPAS
                     RYTVHSENQIVATAPPAIGKENAPITITTIAGSATFPQPFSYRGCLVPKLKGVKLKGA
                     KKRLRDAECLPGKVRRAQGVGANTGRVIKQKPRPGTLLAPGARVFMKLG"
     gene            1957..2298
                     /gene="rplS"
                     /locus_tag="VFB52_14280"
                     /pseudo
     CDS             1957..2298
                     /gene="rplS"
                     /locus_tag="VFB52_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            2739..>3268
                     /gene="lepB"
                     /locus_tag="VFB52_14285"
     CDS             2739..>3268
                     /gene="lepB"
                     /locus_tag="VFB52_14285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZO07554.1"
                     /translation="MLALALALGIQAWIVKPYQIPSESMEPTLDVGQRVLVNRFIYHF
                     EDPSVGDIVVFHPPVGADNGTECGVMPKNGEPCPEPTAGESSQNFIKRIVAGPGDTLS
                     VKEGHPVVNGEWKKDEPYTLPCGNAGACNMPRTITIPADHYFMMGDNRGASDDSRFWG
                     PVPRDWLIGKAFATYWP"
BASE COUNT          566 a         1152 c         1090 g          460 t
ORIGIN      
        1 catccccacc tcctgcgggc ctccctgcac gatcgtgcag acccagatgc ccggcccggc
       61 cttcgccgct tccccggtga ccggcacggt gatccgctgg cgcatgcagg gcggctcacc
      121 gtcctacccc taccggctgc gcgtcgtctc gcgctcggga tcgacctaca ccgcgaccgg
      181 ctccagcccg tccgtagtgc cactggggcc ggccgcccag gcatttccgg tcagcatccc
      241 gatccaggcg gggcaattga tcggaatcga cctccagcca agcgctccgc tcgatcgacg
      301 ggagttcgtt cccggcgccg cctacgccta catcacgccc cctgtggcgg acggggttcc
      361 gacgccgggg gtcgaggcgc cggaattccc ctacgagtgg gcgatcaacg ccgacgtctt
      421 gcccccaccg tcgatcgcct cgatcgctcc ggcccagggc atcctcgcca gcggctcgac
      481 cgtcaaggtg aagatcaccg gcaccaactt cgccgaagtc accggcgtca ccttcggcgg
      541 tgtgccgacg acctacacgg tcgactccga ctcccagatc accgcgctcc cggcggccca
      601 gaagaaaccg ggcccgcagg cggtcaccgt gaccacgctc gccggcaccg cgaccgcgcc
      661 caagccctac cgctacaccg cctgcgtcgt gccgaagctg gccggcaaga agctgaaagc
      721 cgccaagcgg ctgatccgca agggcgaatg caaagttggc ttcgtcaaga agcgcggcga
      781 ggcgaccgcg aaaaccggcc gcgtcgtcgc gcagaaaccg cccgcagggc gcacggtgcg
      841 caccgggtcc aaggtgcgag tgacgttggg ctagccggat ggcgctcgct cgctgcccga
      901 tatctgctcg ccgcgtgctg ctcgccgttg ttgtcggcgc gatcgttttt gccgtcccgc
      961 ggcccgcgga cgccgggatc gtcacggtcg gcaacccgct ggacaaaggc tcggcgttct
     1021 cgattgcgtc cttcgcgggc tcaaccttcg tcaacggcac gcgcaccggt cccggtgcgc
     1081 tcgccgcctc gccggtcgac ggggtgatcc tgcgctggcg cacccggggg aactacatcg
     1141 gcggcccgtt ccggctgcgc gtggtgcggc cggtgggcag cgggttcgtc ggcgccggga
     1201 ccagcgctcc ggtcacgccg ctcggcgaag ccctgcaggc gttcccgacc cagctgccga
     1261 tcagggcggg cgacctgatc ggcatcgaca ccagcgcgaa aacggacttc gtcgccacca
     1321 ccgacccggt cgccgggacc gacatgacga tcatctcccc gccgctcgtc gacggcggcc
     1381 cgacccagac gggaacgacc agccataact tctacgaact catgatcaac gccgacgtcc
     1441 agccggctcc gaagatcacg cagctgggcc cgctcgatgg ctcggtcaaa ggggggacgg
     1501 aagtgaacgt gatcggcacc gacttcactc gggtctcggg cgtctccttc ggcagcctgc
     1561 ccgcctcgcg ctacaccgtg cacagcgaaa accagatcgt cgccacggcg ccgccggcga
     1621 tcggcaagga aaacgcgccg atcacgatca ccacgatcgc tggctcggcg acctttccgc
     1681 agccgttctc ctaccgcggc tgcctcgtcc cgaagctcaa gggtgtgaag ctgaaagggg
     1741 ccaagaaacg gcttcgcgat gctgaatgcc tgcccgggaa agtgcgccgg gcgcagggcg
     1801 tgggggccaa caccggcagg gtgatcaagc agaagccgag gccaggaacc ttgcttgccc
     1861 caggggcgag agtcttcatg aagctgggct gagctaccat tccgcgaccg cgcggttccc
     1921 gcttccgggt cccggcggtt ttttctcgcc aaaactatga gctccgtaat cgacagcatc
     1981 gagcgcgccc agctgaagca gggcctgccg ccctttgacc cgggcgatcg cgtccgcgtc
     2041 cacttccagg tgatcgaggg ttcgcgcaaa cggatccagg tcttcgaggg cgtcgttctg
     2101 cgccgccagg gctcgggagc ccgcgagacc ttcaccgtgc gcaagcagtc gttcggggtc
     2161 ggcgtcgagc ggacgttccc gctgcactcg ccgaagatcg agaaactcga ggtcgccgcg
     2221 cgcggtgacg tgcgccgggc caagctctac tacctgcgcg gccgcgtcgg caaggccgcc
     2281 cgcgtcgccg agcggcgctg gggcatcgac gaggagatgg tctcgacccc ggtcgaggcg
     2341 accgacgccg agggcgtcag ccaggaggag gtcgtgccgg tggagacgcc ggaggaggcc
     2401 accccggagg ctgaggccag cgaggatgcg cccgaggctg aggccacgga ggccgaggag
     2461 gcgcctgccg aggaggccga ggcgagcgag gagactgaga ctgagaccgc cgagccagag
     2521 gctgcggcgg cggaggattc tggggagact gaggcggcag acgcggaagc ggcagcgccc
     2581 gaagcagggg agactgagga gaacgttgcg tccgagaatg ccgaggacga ggagccggta
     2641 gaggccggcg actccgacga ggacgcaaaa gcctgaaacg cctgatcccg aagccgaaga
     2701 gcaaagccgg cggggtcgtt gagttagtcg tcatcgtcgt gctcgccctg gcgctcgcgc
     2761 tgggcatcca ggcctggatc gtcaagccgt accagatccc ctcggaatcg atggagccga
     2821 ccctcgacgt cggccagcgg gtcctcgtca accgtttcat ctaccacttc gaagacccct
     2881 cggtcggcga catcgtcgtc tttcacccgc cggtcggcgc tgacaacggc accgaatgcg
     2941 gcgtgatgcc gaaaaacggc gaaccctgcc ctgagccgac cgccggggag tcgagccaga
     3001 acttcatcaa gcggatcgtt gccgggcccg gagacacgct ctcggtcaaa gaagggcacc
     3061 cggtcgtgaa cggggagtgg aagaaagacg agccctacac gctgccctgc gggaacgccg
     3121 gcgcctgcaa catgccgcgg acgatcacga tcccagccga ccactacttc atgatgggcg
     3181 acaaccgtgg agccagcgac gacagccgtt tctggggacc cgtcccacgc gactggctca
     3241 tcggcaaggc ctttgccacc tactggcc
//
LOCUS       DASRIG010000536         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2580323, whole genome shotgun sequence.
ACCESSION   DASRIG010000536 DASRIG010000000
VERSION     DASRIG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580323"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="trmD"
                     /locus_tag="VFB52_14290"
     CDS             complement(<1..689)
                     /gene="trmD"
                     /locus_tag="VFB52_14290"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZO07555.1"
                     /translation="MKLDIFTLFPEAFDWFRGQRSVTNAIAEGNELRLVNYRDTTPIN
                     AGKVDDSPYGGGAGMVLRVDVVDAALEATYGSERPRIVLLSPTGPVLDDAIADELVAE
                     PHVALLCGRYEGFDDRVHQHLADDELSIGRYVLAGGELPAMVVADVLMRKLPGALGHE
                     DSALEESFSHQLEGMPEYPHFTRPPSYRGWEVPEVLLSGDHGKVREWRLEQSRRRARE
                     FEQRETRREAG"
     gene            complement(706..936)
                     /locus_tag="VFB52_14295"
     CDS             complement(706..936)
                     /locus_tag="VFB52_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZO07556.1"
                     /translation="MTELLEFLVKALVEEPEAVVVEELEEDGDLVYEITVADGDLGRV
                     IGKGGRVANAIRTIAKAAAVRIDRRVIVDILD"
     gene            complement(949..1203)
                     /gene="rpsP"
                     /locus_tag="VFB52_14300"
     CDS             complement(949..1203)
                     /gene="rpsP"
                     /locus_tag="VFB52_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZO07557.1"
                     /translation="MAVRIRLRRVGSKKNPIWRIVVADKRSPRDGRTIETIGQYNPQT
                     EPSTIVLDEERAKHWLEHGAQPSETVAGLLRTKGIKASGS"
     gene            complement(1236..>2137)
                     /locus_tag="VFB52_14305"
     CDS             complement(1236..>2137)
                     /locus_tag="VFB52_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZO07558.1"
                     /translation="HVYEQGTETDPVEIASWALDRAREERRDVLIIDTAGRLHIDQEL
                     MGELSEIRKKTRPHDVLLVVDAMTGQDAVGVAESFAEAAEFDGVVMTKLDGDARGGAA
                     LSVKAVTGKPILFASTGEKLEDFDKFHPDRMAQRILGMGDVLSLIEKAEQEVDEQDAL
                     DLQEKLKKDEFTLDDFLKQMKQVRKMGPLSGLLGMLPGMGAMKQLKQANIDERELDRT
                     EAIVLSMTPAERADPTLINGSRRKRIANGSGTRVQHVKQLIKQFDQMRVLMRSMANGK
                     MPSEHQIAQMAMGGTKRPKVRRR"
BASE COUNT          286 a          723 c          738 g          390 t
ORIGIN      
        1 tagcccgctt cgcggcgcgt ttcgcgctgc tcgaactcgc gggcgcgccg gcggctctgc
       61 tccaggcgcc actcgcgcac cttgccgtgg tcgccggaga gcagaacctc ggggacctcc
      121 cagccgcggt aggacggcgg acgggtgaag tgcggatact cgggcatgcc ctcgagctgg
      181 tggctgaagg actcctcgag cgccgagtcc tcgtggccga gcgcaccggg cagcttgcgc
      241 atcagcacgt cggcgacgac catcgccggc agctcgcccc cggccagcac gtagcggccg
      301 atcgagagct cgtcgtcggc gaggtgctgg tggacgcggt cgtcgaagcc ctcgtagcgg
      361 ccgcagagca gcgccacgtg cggctcggcg accagctcgt cggcgatcgc gtcgtcgagc
      421 accgggccgg tcgggctgag caggacgatc cgcggccgct cggacccgta ggtcgcctcc
      481 agcgccgcgt cgacgacgtc gacccgcagc accatcccgg cgccgccgcc gtagggcgag
      541 tcgtcaactt tgccggcgtt gatcggggtc gtgtcgcggt agttgaccag ccgcagctcg
      601 ttgccctcgg cgatcgcgtt ggtcaccgag cgctgcccgc gaaaccagtc aaaggcctcg
      661 gggaagagcg tgaagatgtc gagcttcacc ggctcgccgc cgagctcagt cgaggatgtc
      721 gacgatgacc cggcggtcga tgcgcaccgc cgcggccttg gcgatcgtgc ggatcgcgtt
      781 ggcgacgcgg ccacccttgc cgatcacacg gccgagatcg ccgtcggcga cggtgatctc
      841 gtagaccagg tccccgtcct cttcgagctc ctcgaccacg acggcctcgg gctcctccac
      901 cagggctttg acgaggaact cgagcagctc cgtcatcggc caggccggct agctgccgga
      961 agctttgatg cccttggtgc gcagcaggcc ggctacggtc tccgacggct gggcgccgtg
     1021 ctcgagccag tgcttggcgc gctcctcgtc gaggacgatc gtcgagggct cggtctgcgg
     1081 gttgtactgg ccgatcgtct cgatcgtgcg cccgtcacgc ggcgagcgtt tgtcggcgac
     1141 gacgatgcgc cagatcgggt tcttcttcga acccacgcgt ctaagtctga ttcggactgc
     1201 cataggacgc ggcaggttag cggaccaggc gcgcgttagc gccgtcggac cttcgggcgc
     1261 ttcgtcccgc ccatcgccat ctgggcgatc tggtgctcgc tcggcatctt gccgttggcc
     1321 atcgagcgca tcagcacgcg catctggtcg aactgtttga tcagctgttt gacgtgctgc
     1381 acacgggtcc cggagccgtt ggcgatccgt ttgcggcgtg agccgttgat cagggtcggg
     1441 tcggcgcgct cggccggggt catcgagagc acgatcgctt ccgtgcggtc gagctcgcgc
     1501 tcgtcgatgt tcgcctgctt gagctgtttc atcgccccca tgccaggcag catcccgagc
     1561 aggccgctga gcgggcccat tttccggacc tgcttcatct gcttcaggaa gtcgtcgagc
     1621 gtgaattcat ccttcttgag tttctcctgg aggtcgagcg cgtcctgttc gtcgacctcc
     1681 tgctcggctt tctcgatcag gctgagcacg tcgcccatcc cgaggatgcg ctgggccatc
     1741 cggtcggggt ggaacttgtc gaagtcctcg agcttctcgc cggtcgaggc gaagaggatc
     1801 ggcttgccgg tcaccgcttt gaccgagagc gcggcgccgc cgcgggcgtc gccgtcgagc
     1861 ttggtcatca ccacgccgtc gaactcggcc gcctcggcga acgattcggc gaccccgacc
     1921 gcgtcctggc cggtcatcgc gtcgacgacc aggaggacgt cgtgcgggcg cgtcttcttg
     1981 cggatctccg agagctcgcc catcagctcc tggtcgatgt ggagacggcc ggcggtatcg
     2041 atgatcagca cgtcgcggcg ctcttcgcgg gcgcggtcga gggcccagga tgcgatctca
     2101 acggggtcgg tctcggtgcc ctgctcgtag acgtggg
//
LOCUS       DASRIG010000537         5446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3120386, whole genome shotgun sequence.
ACCESSION   DASRIG010000537 DASRIG010000000
VERSION     DASRIG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120386"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFB52_14310"
     CDS             <1..1056
                     /locus_tag="VFB52_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO07559.1"
                     /translation="PPALLDFEQARLRLDRMASRVPPEQPWLTPKARRWDGQTFESWV
                     RSNTVTASARTLFELVTEAVWACEPADLSLLHVLFYVRAAGGRLNMLLGTNGGAQQNR
                     FRGGPQQIPERIAAELGAVLTLSAPVRRIEYGEGSVVLHGGGREGEARRVVEARRAIV
                     AIPPTLAGRIAYDPPLPPARDQLTQRMPQGTVIKTMAIYERPFWRDAGLSGQAVSQEG
                     PARVTFDNSPPGGRPGVLLGFIEGRLAREWGSRPASERRDAVLAGHARLFGAEAATPE
                     RFIEQVWAAEEWTRGCYGCLMTPGGWTGFGAALRAPVGPLHWAGAETATRWNGYMDGA
                     VQSGERAASEILAAIGH"
     gene            1217..2425
                     /locus_tag="VFB52_14315"
     CDS             1217..2425
                     /locus_tag="VFB52_14315"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07560.1"
                     /translation="MTDRQQTSGLEIVTLATIGCRLRERRREIEEAILGQLLANESEL
                     RIDEEELRRGMQETVRACLDLVLLTIGNDPDWDDLPMVPPAAVVQTRRVAQSGFGLNG
                     MLQTYIGGYSVLWDFVLEEIKRVEVNAEERAELLRLSSVRLASMAAQLLPAVAAAHAR
                     ESNRIRQSREERTVGIVQALLKSSLVDADELAYDVDGSHVGLIATGPLAVKVVQTVAK
                     TLGRGLLHVCRDDGVVWGWLSSDEPVSPTGFPDLGPVASGVRLAFGEPASGRNGFRST
                     HRQAQAAMQVALCQRQLVTRYADVLLLVPAIQDEQAGDSLISIYLSPLEESRERSPTL
                     KDTLQAYFEAERNTSATAARLKVDRRTAMTRLRTVEERLGFPIYKRHAELEVALRLDQ
                     LRREARAQDE"
     gene            2501..>5446
                     /locus_tag="VFB52_14320"
     CDS             2501..>5446
                     /locus_tag="VFB52_14320"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZO07561.1"
                     /translation="MEKTFRLAIVGAGPSCTYIVERLAATASQLADGLRLHLDVFDRS
                     GQFGAGSVHSTEQPRTSYLNRIVGQVAFAADESVQGAGPLLDAEQRPTLHEWCRRRFE
                     QTGDPTFDLAAEDWPKRYVHGLALRESFQRFVEILASHEGVTVELHETEVVDLVDRGQ
                     RLELLTADGDARLADHVVLATGHSANQPLRPRERELAEFAARSATTYVPSAYPLEEHV
                     VGEVAAPGSVVACNGMGLTAIDIALYLTEARGGRFERAENGRLRYLPSGREPRLIVPF
                     SESGLFTFARPYNGKEVDLDRFQHRSVFLTREAIDRLRASVGRPGADAERPQIDFERD
                     VFPVLLLEMAHLYYRTLLGEELGAQLRECATPAYERFLDGDVGGGAASLLAAADQIFD
                     EAVQLLDPVLAGTSDHAVLSRGERSWSAERAFRRHLEVVFGAEQGARAGDRIAGGEPV
                     REALDGLASAHGHELELAGKRFSWEAAIEPIAAADQGDPESYRRAMLEFIERDHRWAA
                     QGNLENPAKAAADGVWRDSRPVLAYAVDFGGLTAASHRAFLETYMRHHNRLANGAGLE
                     VMERIQALAEHGLLDVSIGPEARVDLDEDAGCFRIVGGRTGAVVEADTLIGGTVHPFD
                     PERDVLPLYPNLLRRGLIRKWRNADAAGGEAYEPGGLDLSSRFHPRRADGEVDGRLTL
                     LGPPSEGVMFFQLGALRPNQDHHVMRDILCWLEDFRALVETGGEGDEAGAAAASEPAG
                     EVVGSSISISEESPAASLRRQLARPRLLSVGGVHDALSALVAERCGLDALWASGLGIA
                     ASHGVADANILTLGEVRDAARLIAGASRLPVIADCDTGFGEVRNLRRTIAEFEHAGVA
                     AVCIEDKLYPKRNSFGPASGQQLIDPYEFATKIRAAKDTQRSSELMVVARLESLIAGE
                     TVEEALGRARLYVAAGADAIVVHSKAKTPDQVIEFARRWRAEGFVTPLIAIPTTYHRV
                     TAEQLERAGF"
BASE COUNT          781 a         1929 c         1947 g          789 t
ORIGIN      
        1 ccgcccgccc tgctcgactt cgagcaggcg cgcctgcgcc tggaccggat ggcctcccgg
       61 gtgcccccgg agcagccttg gttgaccccg aaagcgcgcc gctgggacgg ccagacgttt
      121 gagtcctggg tccgctccaa caccgtcacc gcctccgctc gcaccctctt cgaacttgtc
      181 accgaggcgg tctgggcctg cgagcccgct gatctctcgc tgctgcacgt gctcttctac
      241 gtgcgcgcgg ccggcggtcg tctcaacatg ctgctcggca ccaacggggg tgcccagcag
      301 aaccgcttcc gcggcgggcc gcagcagatc cccgagcgga tcgccgccga gctcggcgcc
      361 gtcctgacgc tctcggctcc ggtgcgccgg atcgagtacg gggaaggcag cgtcgttctt
      421 cacggcggcg gtcgcgaggg cgaggcgcgg cgcgtggtcg aggcgcggcg ggcgatcgtc
      481 gcgatcccgc ccaccctggc tggtcgcatc gcctacgacc cgccgctgcc acccgcccgc
      541 gatcagctca cccagcggat gccccagggg accgtgatca agacgatggc gatctacgag
      601 cgccccttct ggcgcgacgc cggcctttcc gggcaggcgg tcagccagga ggggccggcc
      661 cgggtcacct tcgacaactc gccccccggc gggcggccgg gcgtgctgct cggcttcatc
      721 gagggtcgtc tggcgcgcga atggggctcg cgccccgcca gcgagcgccg cgacgccgtg
      781 ctcgctggtc acgccaggct gttcggcgcc gaggcggcga cgccggagcg cttcatcgag
      841 caggtgtggg cggcggagga gtggacccgc ggctgttacg gctgcctgat gacgccgggt
      901 ggctggaccg gcttcggggc tgcgttgcgc gccccggtcg gccccctgca ctgggccggg
      961 gcggagaccg caacccgctg gaacggctac atggacggtg cggtgcagtc gggcgagcga
     1021 gcggcgtcgg aaattcttgc ggcgatcggg cactgagccc ggcgcttctg caacaagggg
     1081 atcgggagtg ggttggggaa ccaaatgcga actggaagca gctataagta ctcagctttt
     1141 gttgtgagcg gggggagcac gcacagctcg ggggcggttg gagccgtccc ggagaacgag
     1201 tgcctgcgat cacagcgtga ctgacaggca gcagacttcc ggcctcgaga tagtcaccct
     1261 ggcaacgatc gggtgtcgcc tcagagaacg ccgccgcgag atcgaagagg cgattctcgg
     1321 tcagctgctg gccaacgagt ccgagctgcg gatcgacgag gaggaactgc gccggggaat
     1381 gcaggaaacc gtgcgcgcct gcctcgacct ggtcctgctg acaatcggga acgacccgga
     1441 ctgggacgac ctgccgatgg tgcctcccgc ggcagtcgtg caaacgcgcc gggtcgccca
     1501 atcggggttc gggctgaacg gcatgctgca gacatacatc ggcggttaca gcgtgctctg
     1561 ggacttcgtg ctcgaggaga tcaagcgagt cgaggtgaac gccgaggaac gggccgagct
     1621 gctgcgcctc tcttcggtgc ggctcgcctc gatggcggcg cagctgctgc ctgcggtggc
     1681 ggcggcgcac gcgcgtgaga gcaaccggat caggcagtcg cgcgaggagc gcacggttgg
     1741 gatcgtgcag gcgctgctga agagctccct ggtcgatgcc gacgagctgg cctacgacgt
     1801 cgacggttcc cacgtcggcc tgatcgcgac cgggccgctg gcggtgaagg tggtgcagac
     1861 agtggccaag acgttgggcc ggggactgct ccacgtctgt cgcgacgacg gcgtcgtctg
     1921 gggttggctg agcagcgacg agccggtctc cccgacgggg ttcccggacc tcggcccggt
     1981 cgccagcggg gtgcgcctcg ccttcggcga gccggcctcc gggcgcaacg gcttccgcag
     2041 cacccaccgc caggcgcagg cggcgatgca ggtcgccctc tgccagcgac agctggtcac
     2101 ccgctacgcc gacgtgctgc tgctggtccc ggcgatccag gacgagcagg ccggagactc
     2161 actgatctcg atctacctct cgccgctgga ggagtcgcgc gagcgcagcc ccacactcaa
     2221 ggacaccctg caggcttact tcgaagccga gcgcaacacc tccgccacgg cggcgcgtct
     2281 gaaggtcgac cggcgcacgg cgatgacccg cctgcgcacc gtcgaggagc ggctcggctt
     2341 cccaatctac aaacggcacg ccgagctgga agtggcgttg aggttggacc agctgcgccg
     2401 tgaggcacgc gcccaagatg agtaaccgtt actcctagag gtgcacacaa tgtggtggtg
     2461 agtaacacac tccggcggtt tactcatccc aatgcatcgg gtggagaaaa ctttccggtt
     2521 ggcgatcgtc ggcgccggcc ccagctgcac ctacatcgtc gagcgtctgg cggcgacggc
     2581 atcgcagctg gcggacggtt tgcgtctgca cctcgacgtc ttcgaccgca gcggccagtt
     2641 cggtgcgggc tcggtccaca gcaccgagca gccgcggacc agctacctga accggatcgt
     2701 cggccaggtc gccttcgccg ccgacgagag cgtgcaggga gcggggccgc tgctcgacgc
     2761 cgagcagcgg ccgaccctgc acgagtggtg ccggcgccgt ttcgagcaga ccggcgaccc
     2821 gaccttcgac ctcgcggccg aggactggcc gaagcgctac gtgcacgggc tcgccctgcg
     2881 cgagagcttc cagcgcttcg tcgagatcct cgcctctcac gagggggtca cggtcgagct
     2941 gcacgagacc gaggtggtcg acctggtcga ccgcgggcag cggctcgagc tgctgaccgc
     3001 cgacggcgac gcccggctcg ccgatcacgt ggtgcttgcc accggccact ccgccaacca
     3061 gccgctgcgc ccgcgcgagc gcgagctggc cgagttcgcc gcccgctcgg ccaccaccta
     3121 cgtgccctcg gcctacccgc tcgaggagca cgtggtcggc gaggttgccg cgcccggctc
     3181 ggtggtcgcc tgcaacggca tggggctgac cgcgatcgac atcgccctct acctgaccga
     3241 ggcccgtggc ggtcgcttcg agcgcgccga gaacggccgc ctgcggtacc tgccctcggg
     3301 ccgcgagccg cgcctgatcg tgccgttcag cgagtccggc ctgttcacct tcgcccgtcc
     3361 gtacaacgga aaggaggtcg acctcgaccg gttccagcac cgcagcgtct tcctcactcg
     3421 cgaggcgatc gaccgcctgc gcgcgagcgt cggccggccc ggcgccgacg cggagcgccc
     3481 gcagatcgac ttcgagcgcg acgtcttccc ggtgctgctg ctggagatgg cccacctcta
     3541 ctaccgcacc ctgctcggcg aggagctcgg cgcccagctg cgcgagtgcg ccacccccgc
     3601 ctacgagcgc ttcctcgacg gtgacgtcgg gggcggcgcc gcgagcctgc tcgccgccgc
     3661 cgaccagatc ttcgacgagg cggtgcagtt gctggacccg gtcctcgcgg gcacctcgga
     3721 ccacgccgtg ctctcccgcg gtgagcgttc gtggtcggcc gagcgggcct tccgccgcca
     3781 cctcgaagtc gtcttcggag ccgagcaggg ggcacgggcc ggagacagga tcgccggggg
     3841 cgagcccgtc cgggaggcgc tggacgggct cgcttctgcg cacggccacg agctggagct
     3901 ggccggcaag cggttttcct gggaggccgc gatcgaaccg atcgcggcgg cggaccaggg
     3961 cgatcccgag agctaccgca gggcaatgct ggagttcatc gagcgcgacc accgctgggc
     4021 cgcgcagggc aacctcgaga acccggccaa ggccgccgcc gacggcgtct ggcgcgactc
     4081 tcggccggta ctcgcctacg cggtcgactt cggcggcctc accgccgcct cgcaccgggc
     4141 tttcctcgag acctacatgc gccaccacaa ccggctcgcc aacggcgccg gcctggaggt
     4201 gatggaacgg atccaggcgc tcgccgagca cgggctgctc gatgtctcga tcggccccga
     4261 ggcgcgggtc gatctcgacg aggacgccgg ctgcttccgc atcgtcggcg gccgcaccgg
     4321 cgcggtggtc gaggccgaca cgctgatcgg cggcaccgtg cacccgttcg atcccgagcg
     4381 cgacgtgctg ccgctgtacc cgaacctgct gcgccgcggt ctgatccgca agtggagaaa
     4441 cgccgacgcc gccggcggcg aggcctatga gccggggggc ctcgatctct cgtcgcggtt
     4501 tcacccgcgc cgcgccgacg gcgaggtcga tgggcggctg accctgctcg gtccgcccag
     4561 cgagggcgtg atgttcttcc agctcggcgc cctgcgtccg aaccaggatc accatgtgat
     4621 gcgcgacatc ctctgctggc tggaggactt ccgggcgctg gtggagacgg gcggagaagg
     4681 ggacgaggcc ggggcggccg cggcgagcga gccggccggg gaggtggtcg ggtcttcgat
     4741 cagcatctcc gaggagtccc cggccgcctc cctgcgccgc cagctggcgc ggccgaggct
     4801 gctcagcgtt ggtggcgtgc acgacgcgct ctcggccctg gtcgccgagc gctgcgggct
     4861 cgacgcgctc tgggccagcg ggctcgggat cgccgccagc cacggcgttg ccgacgccaa
     4921 catccttacc ctcggcgagg ttcgcgacgc ggcccggctg atcgccggcg ccagccggct
     4981 gccggtgatc gcggactgcg acaccggttt cggcgaggtc cgcaacctgc gccgcacgat
     5041 cgccgagttc gagcacgccg gcgtcgccgc ggtctgcatc gaggacaagc tctacccgaa
     5101 gcgcaacagc ttcggtcccg ccagcgggca gcagctgatc gacccctacg agttcgcgac
     5161 caagatccgc gcggccaagg acacccagcg cagctccgag ctgatggtgg tcgccaggct
     5221 ggagtcgctg atcgccggcg agacggtcga ggaggcgctc ggccgggccc ggctctacgt
     5281 cgctgccggg gccgatgcga tcgtcgtcca ctcgaaggcg aagacgcccg accaggtgat
     5341 cgagttcgcc cgccgctggc gtgccgaagg tttcgtgacg ccgctgatcg cgatcccgac
     5401 cacctatcac cgggtgaccg ccgagcagct cgagcgggcc ggcttc
//
LOCUS       DASRIG010000538         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1557582, whole genome shotgun sequence.
ACCESSION   DASRIG010000538 DASRIG010000000
VERSION     DASRIG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557582"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..914)
                     /locus_tag="VFB52_14325"
     CDS             complement(57..914)
                     /locus_tag="VFB52_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07562.1"
                     /translation="MASISERESREIEAANASGATPVVFIHGLWLLPSSWANWADFYK
                     QAGYAPLTPDWPDDPETVAEARANPDVLARKTLKQVADHTADVIGKLERKPAVMGHST
                     GGLFAQMLAGRGLSAATVAIDPGIFRGVLPLPFSTLKASGPILLNPRNRSRAITLTFD
                     QFKYGWMNALDEDESKRLYDTYHVAGSGIALAQMANANLNPWTESKVDTKNPDRGPLL
                     ILDGEKDHTVPWAIANASYKRQRRNPGVTEIKKMPDRGHSLTIDHGWQEVAQTALDFV
                     KRFAPAKPS"
     gene            1018..1311
                     /locus_tag="VFB52_14330"
     CDS             1018..1311
                     /locus_tag="VFB52_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07563.1"
                     /translation="MADEHERNDSTGEGSGVEEPGQGAARDYEEFHLHSELGHEARRV
                     VAHPAEETHRLAEELAEGEADTTPFIALTGLAIGLAALVVIVIALVLAAIYLI"
     gene            complement(1319..1648)
                     /locus_tag="VFB52_14335"
     CDS             complement(1319..1648)
                     /locus_tag="VFB52_14335"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO07564.1"
                     /translation="MKTVATIAASCPPLLHRALDQDEADELARVLKVLAEPARLRLLS
                     LIQAQPDGEACVCHLVEPLGLSQGTVSHHLKALLTAGLVTREQRGSWAYYRVAPEALG
                     SVRALLA"
     gene            1781..2191
                     /locus_tag="VFB52_14340"
     CDS             1781..2191
                     /locus_tag="VFB52_14340"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZO07565.1"
                     /translation="MKDVPEVLFVCVQNAGRSQMAAGLMNHRAEGKVHVRSAGSDPAE
                     EINPAVVEAMRELGIDLHGEFPKPLTDEVVRAADAVITMGCGDACPIYPGKRYLDWEL
                     ADPAGQEIETVRAIRDEIDGRVQALLAELVSDEG"
     gene            2191..2871
                     /locus_tag="VFB52_14345"
     CDS             2191..2871
                     /locus_tag="VFB52_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HZO07566.1"
                     /translation="MPLGRRLAAEALGTFALVFFGAGAIMVAAEHGSFGQLGIAIAFG
                     LVITTMIYALGHISGAHLNPAVSFGFALSRHFPWSHVGAYWIAQCFGAIVAALLLRAS
                     LGDTADVGATQPSGSNAQSFLWEVVLTFFLMLVIMAVATDTRAVGEAAAIAVGGTVGL
                     CALVGGPVSGASMNPARSLGPALAAGELGALWIYLLAPLVGAALGALAYQLLRGEPAG
                     MARAAGDI"
     gene            complement(2883..3710)
                     /gene="arsM"
                     /locus_tag="VFB52_14350"
     CDS             complement(2883..3710)
                     /gene="arsM"
                     /locus_tag="VFB52_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HZO07567.1"
                     /translation="MSTETTASADELREQVRRRYAEAARTVTEGSAAGCGSGSCCESD
                     PPGAFGEVLYSADEKGELPDAAALASLGCGNPTAVADLNEGEVVLDLGSGGGIDVILS
                     ARRVGPTGTAYGLDMTDEMLALAQQNAREADVPNVHFLKGLIEAIPLPADSVDVVISN
                     CVINLSTDKAAVLTEMFRVLRPGGRIGITDVVTEDRLSAEERLERGDFAGCIVGAMAL
                     GEYTAGLEAAGFEDVSVTFTHEITDGIHGAIVKASKPNDPAARGLPVVQPAASAGCC"
     gene            complement(3707..4156)
                     /locus_tag="VFB52_14355"
     CDS             complement(3707..4156)
                     /locus_tag="VFB52_14355"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZO07568.1"
                     /translation="MIQRRDEAATAAGGIDQTSLICITSTFFDMMFVEMTATTLDVIA
                     CCRPLAAARLSDDDATATATLFKALADPARVKIVNMLATRDEPVCACEFHPALGLSQA
                     TASHHLKKLTDAGLLEREQRGKWAYFSLNPEAVAGLAGLVDFPEVAR"
BASE COUNT          696 a         1542 c         1633 g          774 t
ORIGIN      
        1 ccgcgacggt taccggcgag ccgaccgcgc cctggcgaga ggcttaccta cacgaactag
       61 ctcggcttgg ccggggcgaa ccgcttgacg aagtcgagcg cggtctgcgc gacctcctgc
      121 cagccgtggt cgatggtcag cgagtggccg cggtcgggca tcttcttgat ctcggtgacg
      181 cccgggttgc gcctctgccg cttgtaggag gcgttggcga tcgcccaggg aaccgtgtgg
      241 tccttctccc cgtcgaggat cagcagcggg ccgcggtcgg ggttcttcgt gtcgaccttc
      301 gactcggtcc aggggttgag gttggcgttg gccatctggg ccagggcgat ccccgacccg
      361 gcaacgtggt aggtgtcgta gaggcgcttc gactcatcct catcgagggc gttcatccag
      421 ccgtacttga actggtcgaa ggtgagcgtg atcgcgcggc tcctattccg ggggttcaac
      481 aggatcggcc cactcgcctt gagcgttgag aaaggcagag gcaggacgcc gcgaaatatg
      541 cccgggtcga tggcaaccgt ggctgccgag agcccacggc cggcgagcat ctgggcgaac
      601 aatccgccgg tcgagtgccc catcacagcc ggcttcctct cgagcttgcc gatcacgtcg
      661 gccgtgtggt cggcaacctg cttcagcgtc tttctggcga gaacgtccgg gttcgcacgc
      721 gcctctgcaa ccgtttccgg atcgtcgggc cagtcaggcg tcagcggtgc gtaaccggcc
      781 tgcttgtaga agtccgccca gttggcccag ctgctcggca gcagccagag gccgtggatg
      841 aagacgaccg gggtggcgcc ggaggcgttt gctgcctcga tctccctgct ctcccgctcc
      901 gaaatggatg ccatgtcgga ttcctttcgg ccgcagtaga aggtcacccg accctagcga
      961 atcgactctg cagccacagg ggtggatcta ccgcgcatgt cttgtaagac tcacggcgtg
     1021 gcagacgagc acgagcggaa cgattcgacc ggtgagggct cgggggtcga ggagccgggc
     1081 cagggagccg ctcgagatta cgaggagttc cacctgcata gcgagctcgg gcatgaggca
     1141 aggcgggtag tcgcgcaccc ggccgaagaa acccacaggc tggccgagga actcgcggag
     1201 ggcgaggcgg acaccacccc gttcatcgcg ctcacggggc tggcaatcgg gctcgcagcc
     1261 ctcgtcgtca tcgtgatcgc gctcgtcctg gctgcgatct acctgatctg agaaagccct
     1321 aggcgagcag cgctctgacc gacccgagcg cctcgggcgc gacccggtag tacgcccagc
     1381 ttccacgctg ctcgcgggtc acgaggccgg cggtgagcag cgccttcaag tggtggctga
     1441 ccgtcccttg cgagagcccg aggggctcaa cgaggtggca gacgcaggcc tcgccatcgg
     1501 gctgggcctg gatcaggctc aggagccgga gccgggccgg ctcggcgagc accttcagga
     1561 cgcgcgccag ctcgtcggct tcgtcctggt cgagcgcccg gtgaaggagc ggcgggcagc
     1621 tggcggcgat cgtcgccacg gtcttcatct ggtgaaacac attgacagcc atagatgtcc
     1681 ttgtgaagat cgggcatcga cggcgatcaa tctccggaag tgcgcgcatc gcgtcgcccg
     1741 tgagcgactc gaggccctgg gccaggcgga agggaggatc gtgaaggacg tccccgaggt
     1801 gctctttgtc tgcgtccaga acgccggacg ttcgcagatg gccgcaggcc tcatgaacca
     1861 ccgggcagag ggcaaggtgc acgtccgctc tgccggctcc gacccggccg aggagatcaa
     1921 cccggccgtc gtcgaggcga tgagggagct cgggatcgac ctgcacgggg agttcccgaa
     1981 gccgctcacc gacgaagtag ttcgcgccgc ggacgcggtg atcacgatgg gctgcggcga
     2041 tgcctgcccg atctacccgg gcaagcgcta cctcgactgg gagctcgcgg atccggccgg
     2101 ccaggagatc gagaccgtgc gcgcgatccg cgacgagatc gacgggcgcg tgcaggcgct
     2161 cctcgccgag ctcgtttccg acgaaggctg atgccgctcg gccgccggct cgccgccgag
     2221 gcgctcggca cgttcgccct cgtgttcttc ggcgccggcg cgatcatggt cgccgccgag
     2281 cacggctcct tcggtcagct cgggatcgcg atcgccttcg gcctcgtgat cacgaccatg
     2341 atctatgcgc tcggtcacat ctccggcgca catctgaacc cggccgtctc cttcggcttc
     2401 gccctcagcc gacatttccc ctggagccac gtcggcgcct actggatcgc gcagtgtttt
     2461 ggcgcgatcg ttgctgcgct cctcctccgc gcatccctgg gcgacaccgc cgacgtcggc
     2521 gccacccagc cttctggctc gaacgcgcag agcttcctct gggaggtcgt gctgacgttc
     2581 tttctgatgc tcgtgatcat ggccgtcgcc accgacaccc gcgccgtcgg cgaagcggcg
     2641 gcgatcgcgg tcggaggcac ggtcggcctg tgcgccctcg tcggcgggcc tgtcagcggc
     2701 gcctcgatga acccggcccg ctcgctcggg cccgcgctag cagcaggcga gcttggcgcc
     2761 ctctggatct acctgctcgc cccgctcgtc ggcgcagcac tgggcgcgct cgcctaccag
     2821 ctccttcggg gcgagcctgc cggcatggct agagcagccg gcgatatcta gcgcccccgt
     2881 gctcagcaac agccggcgct cgcggcgggc tggacgacag ggaggcccct ggcggccggg
     2941 tcgttcggct tgctcgcctt cacgattgcg ccgtggatgc cgtcggtgat ctcgtgcgtg
     3001 aaggtgacgc tcacgtcctc gaagccggcg gcctcgagac cggcggtgta ctcgcccagt
     3061 gccatggcgc cgacgatgca gccggcgaag tcgccccgct cgaggcgctc ttccgcgctc
     3121 agtcggtctt cggtgacgac gtcggtgatg ccgatccgcc cacctgggcg gagcacgcgg
     3181 aacatctcgg tcagcacggc cgccttgtcg gtcgagaggt tgatcacgca gttggagatg
     3241 acgacgtcga ccgagtcggc cggaagcggg atcgcctcga tcagcccttt gaggaagtgc
     3301 acgttcggaa cgtcggcctc gcgcgcgttc tgctgggcga gggcgagcat ctcgtcggtc
     3361 atgtcgaggc cgtaggcggt accggtcggc ccgacgcgcc tcgcggaaag gatcacgtcg
     3421 atgccgccgc ccgaaccgag gtcgaggacg acctcgccct cgttcagatc ggcaacggcg
     3481 gtcgggttgc cgcacccgag gctcgcgagc gccgctgcat cggggagctc gcccttctcg
     3541 tcggccgagt agagcacctc gccgaacgcg ccgggcggat cgctctcgca gcaggagccg
     3601 ctgccgcagc cggcggcgga tccttccgtg accgtgcggg cggcctctgc gtagcgccgg
     3661 cgtacctgct cgcgcagctc gtcggcggac gcggtcgtct cggtgctcat cgtgcaacct
     3721 ccggaaagtc gacgaggccg gcgagacccg ccactgcctc agggttcagg gagaagtacg
     3781 cccacttgcc gcgctgctca cgctcgagca ggccggcgtc ggtcagcttc ttcaggtgat
     3841 ggctggccgt cgcctggctg aggccgaggg cggggtggaa ctcgcaggcg cagacgggct
     3901 cgtcccgcgt ggcgagcatg ttcacgatct tcactcgggc cggatcggcc agggccttga
     3961 agagcgtggc cgtcgccgtc gcgtcgtcgt cggaaaggcg tgccgcggcg aggggccggc
     4021 agcaggcgat cacgtcgaga gttgtcgcgg tcatttcgac gaacatcata tcgaaaaacg
     4081 tcgatgttat gcaaatgagg ctggtctgat cgatgcctcc ggcggcggta gccgcctcgt
     4141 cgcgtcgttg gatcaccaga gccgggctga ccccatcgcc cggctctgaa aaggactgct
     4201 ccgctggggg gacagagggt gtcgaccccg gggtgccagc ctggcgacgt ggtctgcatc
     4261 cactcgcggc agtccggttg gcggccagat tcccgcgcgg cggggaggtc aaacgccggt
     4321 tgtcccgcgc gcgcccggtc cgcggtaacg ccgggaagcg gcgcgtctgc ctcccggtcg
     4381 tttgcgcccc gaaagctggg gtaggccgga tggaagtcga aactgagagg aggggtctca
     4441 tggcgaatcc cgtacccgcg ggcagcgacg tttctgcagg gacctacgag tgcacgaact
     4501 gcggcaacag gatcgagatg ggctcgcagt cccacattcc gccttgtcct tcgtgcggcg
     4561 gcggcgagtg gcggacggtg tccggcggcg acagcgtcga agacccgtat ccgggcaact
     4621 gagctgagga tcgaggccgg ccgcg
//
LOCUS       DASRIG010000539        11655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_781078, whole genome shotgun sequence.
ACCESSION   DASRIG010000539 DASRIG010000000
VERSION     DASRIG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781078"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFB52_14360"
     CDS             complement(<1..716)
                     /locus_tag="VFB52_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO07569.1"
                     /translation="MSAATAVTVETGEQLGYARAINAALAEEMRREERVWTLGQDVGR
                     MGGVFGVTRGLYEEFGADRVRDTTLNETFIVGAAVGAALTGTIPIAELQFADFMMIAG
                     DEVFHKMAKWRYMHGANLSVPMVLRLPTGVLGGVGAEHSQSLEALGMHFPGLKVVLPA
                     TPADAKGLLKTAIRDPNPVLYFEHKYLYRRIKEDLPAGEDVLTPIGEARVAREGRDLS
                     VITWSAMLWKSLEAAEKLER"
     gene            complement(713..1681)
                     /locus_tag="VFB52_14365"
     CDS             complement(713..1681)
                     /locus_tag="VFB52_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZO07570.1"
                     /translation="MGARTKANEGPGEAALLALYRNVTRIRALELLLNRYIEKEGFGG
                     FWHPGLGQEGVQAGAVAALREDDYLFYAHRGLGYALAKGMSPVTLLADLFGKRGGSVG
                     GKGGGTVHFVDPSRGVLGQGGTVGSSFVLGAGAGVSSQLLGDDRVTAIFFGDGSSARG
                     TWHEAALTASVWKLPLVWICENNGWAISVPIAEQSPTEDIADRAAAYGVPGVVVDGQD
                     AVAVRDATAEAVARARAGEGPTLIEAKTLRVRGHFEGDKQAYREDVVDGLEVPRDPLD
                     ILRQRLPDAEAERLDEEARAEAEAALEEVLAMPGGDEETVTEGVWV"
     gene            1736..2527
                     /locus_tag="VFB52_14370"
     CDS             1736..2527
                     /locus_tag="VFB52_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZO07571.1"
                     /translation="MASAETSVPRLRLEIRQHVAWLTLGQLERPTVLDEALARELVEA
                     AALIGTDREVRCVVLTGAGKAFALGADIEQIEANTVEQNLHYNRRLRDAADALAALPV
                     PTIAALGGHAIGGGLELALACTLRIAAAGAKLGMPEARLGILPATGGVARLPRLVGTA
                     TAARLLLTGDLIDAAEAARLGVVDTVVAREALAAEAQQLAERIATAAPLSVRALVAAL
                     REDELAIDAANARTELRLADLLGSADRTEGARAFIERREPEFEGR"
     gene            2529..3689
                     /locus_tag="VFB52_14375"
     CDS             2529..3689
                     /locus_tag="VFB52_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07572.1"
                     /translation="MPLPRSAELGLSDEQLLMRRTVRQFAQEKVAPRARALDAEQGFP
                     AESWAEAAELGLLGTTVPSEDGGAGLGLTELCLIGEELAGVCISTAATVLHQADMVVG
                     RIARHGSAAQKQRWLPGLIDGSLVGCLAMTEVEAGSDVMSMRTRAERSEGGWRLSGSK
                     TFITNGPVADLALVYARTPEHGERSLGFFAVPTGAAGFSRGKKFSKMGWRGSPTGELL
                     LDECEVGDDALIGAPDGGRAILLDGLDSERVLMAAESVGLAQGALEVALAYAGERRQF
                     GQPIGQFQLIGAKLADMFAETEAARALTRSLAARIDAGETAGLRALASACKLLGGDLA
                     MRVTTEAVQVLGGYGYIDEFPVERYMRDAKLMQIGGGTAEIQRYVIARELLA"
     gene            3686..4495
                     /locus_tag="VFB52_14380"
     CDS             3686..4495
                     /locus_tag="VFB52_14380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07573.1"
                     /translation="MKLAGARAVVTGGGSGIGAAVARELAARGAAGVLVADLDAEAAA
                     AVAAEIDGIAVQADVGRETEIVALVDRAEQELGGVDLFCSNAGVAGPYGGPEVGDQLW
                     QRTWEINVMSHIWAARRLLPGMLARGSGHLSSTSSAAGLLANPGLMTYSVTKHAALAV
                     AEWLAITYGGPDSGVEFSCFCPQGVATPMLETWREEDPSSRLASASGDTITPEAAATA
                     LADGIEAGRFLILSHAEVQTYTQRKAADPERWLTGMQRFQAEIDRARAELE"
     gene            complement(4539..5261)
                     /locus_tag="VFB52_14385"
     CDS             complement(4539..5261)
                     /locus_tag="VFB52_14385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07574.1"
                     /translation="MAEKAALVTGGSSGIGLAIAKVLVEAGYAVTIASRRPEKLATAA
                     AELRALGGTVVEAPADLSTEQGVVDVVARHRAELGRLDLLVNNAGMGVAGDVGDLQTK
                     HIDLQLALNIKAVILFYREALPLLRAAGAEHGNALVVNVSSITGKRGHRHISVYSATK
                     HAVVGFTQSMDAELAGDGIKSCALCPAYVDTELADYKKGELPAEEMITVDDVAGAVRF
                     LIGLSKTCAVPEIMFTKPGELA"
     gene            5373..6074
                     /locus_tag="VFB52_14390"
     CDS             5373..6074
                     /locus_tag="VFB52_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZO07575.1"
                     /translation="MTEITTPRKRASRRGEIVDTFTRMVAEHGYDAVAIRDLAEDLGM
                     SKGTVLHHFGSKDRLLEEVHSTYMRRRLDEIAIILAELEGPEQRLKGMVFQNLIAMQQ
                     DADATRAFAREIVRFAREDLMKDVRAMRREYFDTLRGILAEGIEQGSFREADTTILAL
                     QMFGMMNWTWTWFDPEGKTSVESIARTFLDTVLHGIEAGAPPADDDAALIELVRGAIA
                     EVAAREAENAAPARA"
     gene            6141..7046
                     /locus_tag="VFB52_14395"
     CDS             6141..7046
                     /locus_tag="VFB52_14395"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZO07576.1"
                     /translation="MREPAKLLGGGSFFEGPRWHDGLWWVSDFYTNGGRILAVDPGGA
                     IEREVPLEQPSGIGWLPGGDLLGVVRTRGELWRLGESGEWEVYADLSEFSRGEANDMC
                     VDAEGRAWVGNFGYDLYAGEPARGATLIRVDPDGSVHAAAEDLHFPNAIIVPPPGDTL
                     IVAETIAARLTAFDIGADGSLSNRRVYAQIAPTPPLEEIDAEYTKVGYGPDGCTLDAE
                     GCIWAGNSLGTEIARIAPGGEILETLPSPVPGHAIYAVQLGGADGRTLLMCVAPDWRL
                     GMGGEEPLASLYTVEVEVPHHGGLP"
     gene            7097..8767
                     /locus_tag="VFB52_14400"
     CDS             7097..8767
                     /locus_tag="VFB52_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO07577.1"
                     /translation="MVTAEEIGTIEVFSSLGPDERESLARLAADISLVSGEYAAHEGD
                     DRAIFAVLGGRIEAVKSTDGVERHVGERHVGDIFGEVPIVLGTVFPVGFRAAEDSRVM
                     KIEPRHYHAIASAEPDLAREVGRLAAHRMTGAHGLESLAADPPPPRALVVGARLDPAC
                     AELRRFLDRNQITFDWITPEDRNAEDKWGGPLPAAPDWPAIRAVGGKTAVRPSQRRVA
                     ELLGLDTEPAHAEYDTVIIGAGPAGLAAAVYGASEGLRTLVVEREAPGGQAGTSSRIE
                     NYLGFPSGVSGDELASRALSQARRLGAEILVTRQITRIDAVNRELHLDGGDVLRARTI
                     ILACGVTWRNLTIEGFIRLAGKGIFYGAARSEAPNTHGLDVHVVGAGNSAGQAALFFS
                     THARSVTVLCRGEGLEKSMSRYLIDQLAARANITTITGAEVQAAHGADALEALDVSDR
                     ASGEVARHDSGGLFIFIGADAETGWLPPEIALDQRGFVLTGNDLTATDHWSLERDPYL
                     LETSVPGVFACGDVRFGPVKRVAAAVGEGSMAIAFVHQYLKELESAAA"
     gene            complement(8764..9576)
                     /locus_tag="VFB52_14405"
     CDS             complement(8764..9576)
                     /locus_tag="VFB52_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07578.1"
                     /translation="MNLSGKVAVVTGGGSGIGEATSARLAADGARVAVVDIDEDAAEL
                     TAKLAGEGLAVVADVTDSAAVDAALVTVEKQLGPVDIWVNNAGIAGGAVSERVNPRAE
                     RQLAEAAVGEVKTALDSLVRMEDEDWRQMLAVHLDGTFYGTRAAARSMAGRKTGSIIN
                     IASICGIEGCTGHPHYSAAKGGILAFTRTVAKELIQQNIRVNAIAPGFVDTQLVSGSL
                     TEPLVAAVVMQTPQGRLGRPEEVAAAVSFLATDDASFFVGDTISPNGGIVTS"
     gene            complement(9573..10673)
                     /locus_tag="VFB52_14410"
     CDS             complement(9573..10673)
                     /locus_tag="VFB52_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HZO07579.1"
                     /translation="MTTGFHWNELYAWHDTGTGSAYMSAGGLVEPEVHGENAATKRRM
                     RNLLDVTGLLERTVPVAGRTATEEELLRVHTPDYVERVKAESEAGGGDGGELAPFGAG
                     GYAICALSAGGVISTIEAVWSGDLDNAYSLNRPPGHHAERDEGRGFCIFASIAVAIEH
                     MRAVHGLERVVVVDWDVHHGNGTEHAFYEDPSVLTISLHQAGLYPAESGLVEHSGKGE
                     GEGFNINVPLPPGSGDGAYVAALERVVVPAIQNFKPQMVIIACGVDASMMDPLAMMCV
                     TSEGYRRMTDVLMAVADEVCDGKLVAIHEGGYSTSYVPFCGAAVIEGLLRSETSVEDP
                     YIAAFRGMEYMDLQPHQEAVIARSAELAAALG"
     gene            complement(10796..>11655)
                     /locus_tag="VFB52_14415"
     CDS             complement(10796..>11655)
                     /locus_tag="VFB52_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZO07580.1"
                     /translation="VAPYLLELTSEAQRERYLPDFCAGKLITAIGMTEPQAGSDLAAL
                     RTRAVRDGEGWLLNGTKTFITNGTACDLVVLAARTGEDRKDVSIFLVEGGTEGFSRGE
                     PLKKIGQQEADTAELFFEDVRLPAEALLGEQGRGFAYMMERLPQERLSVAVANVANAL
                     SVLAETLDYVKERHAFGQPIGRFQSARFTLAELDTELDVGQAFVDRCVEAHVRGELSA
                     VDAAKAKWWTAEMQNKVIDACVQLHGGYGFMDEYRVGRAWADARVTKIYAGTNEIMKD
                     VIGRSLGLG"
BASE COUNT         1625 a         4328 c         4073 g         1629 t
ORIGIN      
        1 tcccgctcca gcttttcggc ggcctccagc gatttccaga gcatcgccga ccaggtgatc
       61 accgaaaggt cccggccttc ccgggccacc cgggcctcgc cgatcggggt gagcacgtcc
      121 tccccggccg gcaggtcttc cttgatccgg cggtagaggt acttgtgctc gaagtagagc
      181 accgggttgg ggtcgcggat cgcggtcttc agcaagccct tggcgtcggc gggggtcgcc
      241 gggaggacga ctttgaggcc ggggaagtgc atgccgagcg cctccagcga ctgcgagtgt
      301 tcggccccga ccccgccgag cacgccggtc ggcaggcgca gcaccatcgg caccgagagg
      361 ttggcgccgt gcatgtagcg ccacttggcc atcttgtgga agacctcgtc gccggcgatc
      421 atcatgaagt cggcgaactg cagctcggcg atcgggatcg tgccggtcag cgcggcgccg
      481 acggccgcac cgacgatgaa ggtctcgttc agggtcgtgt cgcggacccg gtcagcgccg
      541 aactcctcgt agaggccgcg cgtcaccccg aacacgccac ccatccgacc gacgtcctgg
      601 cccagcgtcc agacccgctc ctcgcggcgc atctcctcgg ccagcgccgc gttgatcgcg
      661 cgcgcgtagc cgagctgctc gccggtctcg accgtgaccg cggtcgccgc gctcacaccc
      721 acaccccctc ggtcaccgtc tcctcgtcgc cgccgggcat cgccagcacc tcttccagcg
      781 ccgcctcggc ctcggcccgc gcctcctcgt cgagacgctc ggcctcggcg tcgggcagcc
      841 gctggcggag gatgtcgagc gggtcgcgcg gcacctccag cccgtcgacg acgtcctcgc
      901 ggtaggcctg tttgtcgccc tcgaagtggc cgcgcacgcg cagcgtcttc gcctcgatca
      961 gcgtcggccc ctcacccgct cgcgcccggg cgaccgcctc ggcggtggca tcccgcaccg
     1021 cgaccgcgtc ctggccgtcg accactaccc cgggaacgcc gtaggcggcg gcgcggtcgg
     1081 cgatgtcctc ggtcgggctc tgctcggcga tcggcaccga gatcgcccag ccgttgttct
     1141 cgcagatcca gaccagcggc agcttccaga cggaggcggt cagcgccgcc tcgtgccagg
     1201 tgccgcgggc cgaagagccg tcgccgaaga agatcgccgt gacccggtcg tcaccgagca
     1261 gctgcgagga gaccccggca cccgccccga gcacaaagct ggagccgacc gtgccgccct
     1321 ggccgagcac cccgcgcgag gggtcgacga agtgcacggt gccgccgccc ttgccgccga
     1381 cgctgccgcc gcgtttgccg aacaggtcgg cgagcagggt gaccggcgac atccccttgg
     1441 ccagcgcgta gccgagcccg cggtgggcgt agaagaggta gtcgtcctcg cgcagcgcag
     1501 cgaccgcgcc ggcctgcacg ccctcctggc cgagccccgg gtgccagaag ccgccgaagc
     1561 cctctttctc gatgtagcgg ttgagcagca gctcgagcgc ccggatccgg gtgacgttgc
     1621 ggtagagggc gagcagcgcc gcctcgccgg gtccttcgtt cgcctttgtg cgcgcgccca
     1681 aagcagcgcc ctcctcctgg ttgagactga ccgctcggac ggttagtgta gcccgatggc
     1741 aagtgccgag accagcgtgc cgcgactgcg tttggagatt cgtcagcacg tcgcctggct
     1801 cacgcttgga cagctcgagc gccccaccgt gctcgacgaa gcgctggccc gcgagctggt
     1861 cgaggcggcg gccctgatcg gcaccgaccg cgaggtccgc tgcgtcgtcc tcaccggcgc
     1921 cggcaaagcc ttcgccctcg gcgccgatat cgagcagatc gaggcgaaca cggtcgagca
     1981 gaacctccac tacaaccgcc gcctgcgcga cgccgccgac gcgctcgcgg cgctgccggt
     2041 gccgacgatc gccgcgctgg gcggccacgc gatcggcggc ggcctggagc tggcgctcgc
     2101 ctgcacgctg cggatcgccg ccgccggggc gaagctgggg atgccggagg cgcggctcgg
     2161 gatcctgccg gcgaccggcg gcgtcgcccg cctgccgcgc ctggtcggga ccgcgaccgc
     2221 cgcccgcctg ctgctcaccg gtgacctgat cgacgccgcc gaggcggccc ggctcggggt
     2281 cgtcgacacg gtcgtcgccc gcgaagcgct cgccgccgag gcgcagcagc tcgccgagcg
     2341 gatcgccacc gccgccccgc tctcggtccg cgccctggtc gcggccctgc gcgaggacga
     2401 gctggcgatc gacgccgcca acgcgcgcac cgaactgcgg ctcgccgacc tgctcggcag
     2461 cgccgaccgc acggagggcg cccgcgcctt catcgagcgg cgcgagcccg agttcgaagg
     2521 gcgctgacgt gcccctcccc cgctcggccg agctcggact cagcgacgag cagttgctga
     2581 tgcgacggac cgtgcgccag ttcgcccagg agaaggtggc gccgcgcgcc cgcgccctcg
     2641 acgccgaaca gggcttcccg gccgagagct gggcggaagc ggcggagctg gggttgctgg
     2701 ggacgacggt cccgagcgag gacggcggcg ccggcctcgg cctcaccgag ctctgcctga
     2761 tcggcgaaga gctggccggc gtctgcatct cgaccgcggc caccgtcctg caccaggccg
     2821 acatggtggt cggccggatc gcccgccacg gcagcgccgc gcagaagcag cgctggctgc
     2881 cggggctgat cgacggctcg ctggtcggct gcctggcgat gaccgaggtc gaggccggct
     2941 ccgacgtgat gagcatgcgc acccgcgccg agcgcagcga gggcggctgg cggctcagcg
     3001 gctcgaagac cttcatcacc aacggcccgg tcgccgacct cgccctcgtc tacgcccgca
     3061 ccccggaaca cggcgagcgc tcgctcggct tcttcgccgt ccccaccggg gccgccggct
     3121 tcagccgcgg caagaagttc tcgaagatgg gatggcgcgg ctcccccacc ggcgagctgc
     3181 tgctcgacga gtgcgaggtc ggcgacgacg ccctgatcgg cgcccccgac ggcggccgcg
     3241 cgatcctgct cgacgggctc gacagcgagc gcgtgctgat ggcggccgag tcggtcgggc
     3301 tcgcccaggg cgcgctcgag gtggcgctcg cctacgccgg cgagcggcgc cagttcggcc
     3361 agccgatcgg ccaattccag ctgatcggcg cgaagctcgc cgacatgttc gccgagaccg
     3421 aggcggcccg ggcgctgacc cgctcgctcg ccgcccgcat cgacgccggc gagactgccg
     3481 ggctgcgggc gctcgcctcg gcctgcaagc tgctcggcgg cgacctcgcg atgcgggtca
     3541 cgaccgaggc ggtgcaggtg ctcggcggct acggctacat cgacgagttc ccggtcgagc
     3601 gctacatgcg cgacgccaag ctgatgcaga tcggcggcgg cacggccgag atccagcgct
     3661 acgtgatcgc ccgggagctg ctcgcgtgaa gctggccggc gcgcgggcgg tggtgaccgg
     3721 cggcggcagc ggcatcggcg ccgccgtggc ccgcgagctg gcggcgcgcg gcgcggccgg
     3781 ggtcctcgtc gccgacctcg acgccgaggc ggccgcggcg gtcgccgccg agatcgacgg
     3841 gatcgcggtg caggccgacg tcggccgcga aaccgagatc gtcgccctgg tcgaccgcgc
     3901 tgaacaggag ctgggcggcg tcgacctgtt ctgctcgaac gccggcgtcg ccggccccta
     3961 cggcgggccc gaggtcggcg accagctctg gcagcggacc tgggagatca acgtgatgtc
     4021 ccatatatgg gcggcccggc gcctgctgcc ggggatgctg gcgcgcggct ccggccacct
     4081 cagctcgacc tcctccgccg ccggtctgct cgccaacccc ggcctgatga cctactcggt
     4141 gaccaagcac gcggcgttgg cggtcgccga gtggctggcg atcacctacg gcggtcccga
     4201 ctccggggtc gagttctcct gcttctgccc gcagggagtg gcgacgccga tgctcgaaac
     4261 ctggcgcgaa gaggacccca gctcacgcct cgccagcgcc tccggcgaca cgatcacccc
     4321 ggaggccgcg gccaccgcgc tcgccgacgg gatcgaggcc ggccgcttcc tgatcctctc
     4381 ccacgccgag gtgcagacct acacccagcg caaagccgcc gaccccgagc gctggctgac
     4441 cggcatgcag cgcttccaag ccgaaatcga ccgcgcccgc gccgagctcg agtgaaccac
     4501 ctgacgtttg tcgcccacag ggcgacaagt gcaggcggtt aggccagttc gcccggtttg
     4561 gtgaacatga tctcggggac ggcgcaggtc ttcgagaggc cgatgaggaa gcggacggcg
     4621 ccggcgacgt cgtctacggt gatcatctcc tcggccggca gctcgccctt cttatagtcg
     4681 gcgagctcgg tgtcgacgta ggccgggcag agcgcgcagg acttgatccc gtcgccggcc
     4741 agctcggcgt ccatcgactg ggtgaagccg acaaccgcgt gcttggtcgc cgagtagacc
     4801 gagatgtgcc ggtggccgcg cttgccggtg atcgaggaga cgttgacgac gagcgcgttg
     4861 ccgtgctcgg ccccggccgc gcgcagcagc ggcagcgcct cgcggtagaa caggatcacg
     4921 gccttgatgt tgagcgccag ctgcaggtcg atgtgcttgg tctgcaggtc gccgacgtcg
     4981 ccggcgaccc ccatcccggc gttgttgacg agcaggtcga gccggccgag ctccgcgcgg
     5041 tggcgggcga ccacgtcgac cacaccctgc tcggtgctga ggtcggcggg cgcctcgacc
     5101 acggtgccgc cgagcgcgcg cagctccgcg gccgcggtgg cgagcttctc ggggcggcgc
     5161 gaggcgatcg tgaccgcgta gccggcctcg accagcacct tggcgatcgc cagcccgatc
     5221 ccgctcgatc ccccggtgac cagggctgcc ttctccgcca tctcttcccc ttctcgctcg
     5281 tagttcgtca aactagccgt acggacggtc agtttaggag cgcggggtcg gaccgaaccg
     5341 gatcccgcta gaccggttga taggtatgtc caatgactga gatcacgact ccgagaaagc
     5401 gcgccagccg ccgcggcgag atcgtcgaca ccttcacgcg gatggtcgcc gagcacggct
     5461 acgacgcggt cgcgatccgc gacctcgccg aggacctggg gatgagcaag ggcaccgtgc
     5521 tccaccactt cggctccaag gaccggctgc tcgaggaggt ccactcgacc tacatgcgcc
     5581 gccgcctcga cgagatcgcg atcatcctcg ccgagctcga gggtcccgag cagcgactca
     5641 agggcatggt cttccagaac ctgatcgcga tgcagcagga cgccgacgcg acccgcgcct
     5701 tcgcccgcga gatcgtccgc ttcgcccgcg aggacctgat gaaggacgtg cgcgcgatgc
     5761 gccgcgagta cttcgacacc ctgcgcggga tcctcgccga aggcatcgag cagggctcct
     5821 tccgcgaagc cgacacgacg atcctggcgc tgcagatgtt cgggatgatg aactggacct
     5881 ggacctggtt cgaccccgag ggcaagacct cggtcgagtc gatcgcccgc accttcctcg
     5941 acaccgtcct gcacgggatc gaggcgggcg ccccgcccgc ggacgacgac gccgcgctga
     6001 tcgagctggt ccgcggcgcg atcgccgagg tcgccgctcg cgaagccgag aacgccgccc
     6061 ccgcgcgcgc ttagagatcc tccaagcgcc cgggcccgag ccgccggacg ccccgccccc
     6121 gccgcatagc ctcaatgcgg atgagggaac cggccaagct gttgggtgga gggtcgtttt
     6181 tcgaggggcc gcgctggcat gacggcctct ggtgggtctc agacttctac accaacggcg
     6241 gccgcatcct cgccgtcgac ccagggggcg cgatcgagcg ggaggtaccg ctcgagcagc
     6301 cctctgggat cggctggctc ccaggcggcg acctgctcgg ggtggtgcgg acccgggggg
     6361 agctgtggcg gctgggcgag tccggcgagt gggaggtcta cgccgacctc tccgagttca
     6421 gccgcggcga ggccaacgac atgtgcgtcg acgccgaggg gcgcgcctgg gtcggcaact
     6481 tcggctacga cctctacgcc ggcgagccgg cgcgcggggc gacgctgatc cgggtcgatc
     6541 ccgacggctc cgtgcacgcc gccgccgagg acctgcactt ccccaacgcg atcatcgtgc
     6601 cgccgcccgg cgacaccctg atcgtcgccg agacgatcgc cgcccggctc accgccttcg
     6661 acatcggcgc cgacggcagc ctctccaacc gccgcgtcta cgcgcagatc gccccgaccc
     6721 cgccgctgga ggagatcgac gccgagtaca ccaaggtcgg ctacggcccc gacggctgca
     6781 cgctcgacgc cgagggctgc atctgggccg gcaactcgct cggcaccgag atcgcccgca
     6841 tcgcccccgg cggcgagatc ctcgagaccc tgcccagccc ggtgcccggc cacgcgatct
     6901 acgcggtcca gctcggcggc gctgacgggc gcaccctgct gatgtgcgtc gcccccgact
     6961 ggcggctcgg aatgggcggc gaagagccgc tcgcctcgct ctacacggtc gaggtcgagg
     7021 tgccgcacca cggcggcttg ccctaacgac cgtcacataa gctggcggca atgaacgcgc
     7081 tcgcacgggg accgcggtgg taaccgccga ggagatcggc acgatcgagg tcttctcctc
     7141 gctcggtccc gacgagcgcg agtcgctggc ccggctcgcc gccgacatta gcctggtcag
     7201 cggcgaatac gccgcccacg agggcgatga ccgggcgatc ttcgccgtgc tcggcggccg
     7261 gatcgaggcg gtcaagtcga ccgacggggt cgagcgccac gtcggcgagc gccacgtcgg
     7321 cgacatcttc ggcgaggtgc cgatcgtgct cgggaccgtc ttcccggtcg gcttccgcgc
     7381 cgccgaagac tcgcgggtga tgaaaatcga gccgcgccac taccacgcga tcgcctccgc
     7441 cgagccggac ctggcccgcg aggtcggccg cctggccgcg caccggatga ccggcgcgca
     7501 cgggctcgag agcctcgccg ccgacccgcc gccgccgcgg gcgctggtcg tcggcgcccg
     7561 cctcgacccc gcctgcgcgg agctgcgccg cttcctcgac cgcaaccaga tcaccttcga
     7621 ctggatcacc ccggaggacc gcaacgccga ggacaagtgg ggcggcccgc tgcccgccgc
     7681 gcccgactgg ccggcgatcc gcgcggtcgg cggcaagacc gcggtccgtc cctcccagcg
     7741 ccgggtcgcc gaactgctcg gcctcgacac cgagccggcc cacgccgagt acgacacggt
     7801 gatcatcggc gccggcccgg ccgggctggc ggcggcggtg tacggggcct cggagggcct
     7861 gcgcacgctg gtcgtcgagc gcgaggcgcc gggcggccag gccggtacct cctcgcggat
     7921 cgagaactac ctcggcttcc cctccggcgt ctccggcgac gagctggcga gccgggcgct
     7981 ctcgcaggcg cggcggctcg gggccgagat cctcgtcacc cgccagatca cccggatcga
     8041 cgccgtcaac cgcgaactcc acctcgacgg cggcgacgtg ctgcgcgccc gcacgatcat
     8101 cctcgcctgc ggggtcacct ggagaaatct gacgatcgag ggcttcatcc ggctcgccgg
     8161 caaggggatc ttctacgggg cggcgcgcag cgaggcgccg aacacgcacg ggctcgacgt
     8221 ccacgtcgtc ggcgccggca actcggccgg ccaggcggcg ctcttcttct ccacccacgc
     8281 gcgcagcgtc accgtcctct gccgcggcga gggcctcgag aagagcatgt cgcgctacct
     8341 gatcgaccag ctcgcggccc gcgccaacat caccacgatc accggcgccg aagtgcaggc
     8401 cgcgcacggc gccgacgcgc tcgaggcgct cgacgtcagc gaccgcgcca gcggcgaggt
     8461 cgcccgccac gactccggcg gcctcttcat cttcatcggc gccgacgccg agaccggctg
     8521 gctgccgccc gagatcgcgc tcgaccagcg cggcttcgtg ctcaccggca acgacctgac
     8581 cgcaaccgac cactggtccc tggagcgcga cccctaccta ctggagacca gcgtgcccgg
     8641 cgtcttcgcc tgcggcgacg tccgtttcgg cccggtcaag cgcgtcgccg ccgcggtcgg
     8701 cgagggcagc atggcgatcg ccttcgtcca ccagtacctg aaagagctgg agagcgccgc
     8761 cgcctaagag gtgacgatgc cgccgttcgg gctgatcgtg tcgccgacga agaacgaggc
     8821 gtcgtcggtg gcgaggaagg agaccgcggc ggcgacctcc tcggggcggc cgaggcggcc
     8881 ctgcggggtt tgcatgacca cggcggcgac cagcggctcg gtcagcgagc cgctgaccag
     8941 ctgggtgtcg acgaagcccg gcgcgatcgc gttgacgcgg atgttctgct ggatcagctc
     9001 cttggcgacg gtgcgggtga aggcgaggat gccgcccttg gccgccgagt aatgcgggtg
     9061 gccggtgcag ccctcgatcc cgcagatcga ggcgatgttg atgatcgagc ccgtcttgcg
     9121 gccggccatc gagcgggcgg cggcgcgagt cccgtagaag gtgccgtcga ggtgcacggc
     9181 gagcatctgg cgccagtcct cgtcctccat ccgcaccagc gagtcgagcg cggttttgac
     9241 ctcgccgacc gcggcctcgg cgagctggcg ttcggcccgc gggttgaccc gctcggagac
     9301 ggcgccgccg gcgatcccgg cgttgttgac ccagatgtcg accgggccga gctgcttctc
     9361 gaccgtgacc agggcggcgt cgaccgcggc cgagtcggtc acgtcggcga ccaccgccag
     9421 gccttcgcca gccagcttcg cggtcagctc cgccgcgtcc tcgtcgatgt cgacgacggc
     9481 gacgcgggcg ccgtcggcgg cgagccgggc gctggtcgcc tcaccgatcc cggagccgcc
     9541 cccggtgacc accgcgacct tgcccgagag gttcatccca gcgccgcggc aagctcggcg
     9601 gaacgggcga tcacggcctc ctggtgcggc tgcaggtcca tgtactccat gccgcggaag
     9661 gcggcgatgt acggatcctc gaccgaggtc tcgctgcgca gcagcccctc gatcaccgcc
     9721 gccccgcaga agggcacgta ggaggtcgag tagccgccct cgtggatcgc gaccagcttg
     9781 ccgtcgcaga cctcgtcggc gaccgccatc agcacgtcgg tcatccgccg gtagccctcg
     9841 ctggtcacgc acatcatcgc cagcgggtcc atcatcgagg cgtcgacgcc gcaggcgatg
     9901 atcaccatct gcggcttgaa gttctggatc gcgggcacga cgacccgctc cagcgccgcc
     9961 acgtaggcgc cgtcgccgga gcccggcggc agcggcacgt tgatgttgaa gccctcgccc
    10021 tcgcctttgc cggagtgctc gacgagcccc gattcggccg ggtagagacc cgcctggtgc
    10081 agcgagatcg tcagcacgct ggggtcctcg tagaaggcgt gctcggtgcc gttgccgtgg
    10141 tgcacgtccc agtcgacgac cacgacccgc tcgagcccgt ggaccgcgcg catgtgttcg
    10201 atcgcgacgg cgatgctggc gaagatgcag aagccgcggc cttcgtcgcg ctcggcgtgg
    10261 tggccgggcg ggcggttcag cgagtaggcg ttgtcgagat ccccgctcca gaccgcctcg
    10321 atcgtcgaga tcacgccgcc ggccgagagc gcgcagatcg cgtagccgcc ggcgccgaac
    10381 ggggccagtt cgccaccgtc tcccccgccc gcctcgcttt cggccttgac ccgctcgacg
    10441 tagtcggggg tgtggacgcg cagcagctcc tcctcggtcg ccgtgcggcc ggcgacggga
    10501 accgtccgct ccagcagccc ggtgacgtcg aggaggttgc gcatccgccg cttggtggcg
    10561 gcgttctctc cgtggacctc gggctcaacc agcccgccag cgctcatgta ggccgagccg
    10621 gtgccggtgt catgccaggc gtagagctcg ttccagtgga atcccgtagt cattggccga
    10681 ccgtagttcg cgtcccagaa ccgcccgccg accgattgcg aacgcccctg tagttccccc
    10741 aaaccaccag cccccgcgct gcgcttgtcg cccaccgggc gacaagcgca gggtctcagc
    10801 cgaggccgag ggagcggccg atcacgtctt tcatgatctc gttggtgccc gcgtagatct
    10861 tcgtcacccg ggcgtcggcc caggcgcgcc cgacccggta ctcgtccatg aagccgtagc
    10921 cgccgtgcag ctgcacgcag gcgtcgatca ccttgttctg catctccgcg gtccaccact
    10981 tcgccttcgc cgcgtcgacc gccgagagct cgccgcgcac gtgcgcctcg acacagcggt
    11041 cgacgaaggc ctggccgacg tcgagttcgg tgtcgagctc ggcaagcgtg aagcgggcgc
    11101 tctggaagcg gccgatcggc tggccgaagg cgtggcgctc cttcacgtag tcgagcgttt
    11161 cggcgagcac gctgagcgcg ttggcgacgt tggcgacggc gacgctgagc cgctcctgcg
    11221 gcaggcgctc catcatgtag gcgaagccgc gcccctgctc gccgagcagc gcctcggccg
    11281 gcagccgcac gtcctcgaag aagagctcgg cggtgtcggc ctcctgctgg ccgatcttct
    11341 tcagcggctc gccgcggctg aagccctcgg tgccgccctc gaccaggaag atgctgacgt
    11401 ccttgcggtc ctccccggtt cgggcagcga ggaccacgag atcgcatgcc gtgccgttgg
    11461 tgatgaaggt cttggtcccg ttcagcagcc agccctcgcc gtcgcggacc gcgcgagtgc
    11521 gcagcgcggc caggtcggag cccgcctgcg gctcggtcat cccgatcgcg gtgatcagct
    11581 tgccggcgca gaagtcgggc aggtagcgct cgcgctgggc ctcgctggtc agctccagca
    11641 ggtagggagc gacca
//
LOCUS       DASRIG010000540         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3122276, whole genome shotgun sequence.
ACCESSION   DASRIG010000540 DASRIG010000000
VERSION     DASRIG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122276"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFB52_14420"
     CDS             complement(<1..574)
                     /locus_tag="VFB52_14420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZO07581.1"
                     /translation="MRWDEQKVENDLRIPGVGDGTVTRTFDVPEAMGINFHEVRAKSA
                     LNHVPGGRYGFSWTINPFRGCTHACVFCFARRTHTYLDMDAGRDFEREIVVKVNVPEL
                     LRAELARPGWKRELVALGTNTDPYQWVESRYRMMPEILEALEAAHTPVSVLTKSPLAM
                     RDIEIFERMAKKLPVSVNLSVPTLDEDAWRA"
     gene            complement(662..1150)
                     /locus_tag="VFB52_14425"
     CDS             complement(662..1150)
                     /locus_tag="VFB52_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07582.1"
                     /translation="MGAAENQATIERLYAAFGECNGAAMTACYAPDAHFCDPAFGDLH
                     GDDIGAMWRMLTGRATDLEIELHEHEADEQAGTAHWIARYTFSTGRPVVNDVRARFRF
                     TPDGLIADHVDDFDFRSWCKQALGGAGGFVALIPPLRKKVRAKALDQLATYKREETGS
                     SL"
     gene            complement(1160..1615)
                     /locus_tag="VFB52_14430"
     CDS             complement(1160..1615)
                     /locus_tag="VFB52_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HZO07583.1"
                     /translation="METWSVYLSGEIHSDWRERIEAGVREAGLPVVFAAPVTDHAASD
                     DAGTGTLGEEGEAFWRDHKGAGVNAIRTQTLIRAADLVVVRFGDKYRQWNAAFDAGYA
                     AALGKPIITLHDGELDHALKEVDRAAQATARTAEQVVDVLRYVIAGKLP"
     gene            1650..3170
                     /locus_tag="VFB52_14435"
     CDS             1650..3170
                     /locus_tag="VFB52_14435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO07584.1"
                     /translation="MNVRKRFGTAGVASDHEHYKWWALSCTSLGMLLVATNSGTLIIA
                     LPDLERALDTDLLELVWVILAYMISSTVLVLTAGRLSDLFGRKTAFVAAFLIFAAASL
                     GAGFAADGTQLILWRILQGIGGAFIFANGPALVTDAFPKEQLGLAMGTNTMIAAIGLV
                     IGPVLGGALVAISWEWVFWFNVPLALAGALWAGLVLRELSRRDTVRGLDLLGTSAFVV
                     GLTGLVYGISRGGLSGWDNNLVIGSLIAAAVLLPLFVLIEHRGKAPMLDLTIFQNRLF
                     AAASAATFINGLSRFALMFIFVFYFQGAQGDDPIIAGIKLAPMAVGMLVASPLAGIWA
                     DRHGSRVLAALGMVVTAIGLALMTTLQEDTAYWQALIWLGVVGIGSGMFMSPNTAAMM
                     GTVPTERRGIAAGARTMLQNTGAVISIAFVLAIITAAVPKPVLFKIFSGLASGLTDAQ
                     LDPFIANMHEALWVLAVISVVGAGVSLLRPREQRAPDSDRGLTIDEAIREEIEATP"
     gene            3167..3625
                     /locus_tag="VFB52_14440"
     CDS             3167..3625
                     /locus_tag="VFB52_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO07585.1"
                     /translation="MSPSPARGELRIGEVARLAGTTPRTIRYYEEIGLLPISGGREPG
                     AHRSYAESDVERLTELLRLKDLLGLSLEELKTLAEADEARAALRREWQGGVADPARRE
                     QILEESLGHIGRQLDLVRRRRDEIAALESELTAKRRRVRELLRELPAPTA"
     gene            complement(3622..>3888)
                     /locus_tag="VFB52_14445"
     CDS             complement(3622..>3888)
                     /locus_tag="VFB52_14445"
                     /inference="COORDINATES: protein motif:HMM:NF018287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HZO07586.1"
                     /translation="HYAGFFDPGFGFDPAGNRPGSRAALEVRARDVSFMVEHRQPVCK
                     LALEWMEGPAERLYGADLGSNYQGQVTMLSKHFAEQTAGAPAAA"
BASE COUNT          531 a         1384 c         1346 g          627 t
ORIGIN      
        1 tcgcccgcca ggcgtcctcg tcgagcgtcg gcaccgagag gttgaccgag accggcagct
       61 tcttcgccat ccgctcgaag atctcgatgt cgcgcatcgc cagcggcgac ttggtcagca
      121 ccgagaccgg cgtgtgcgcc gcctccagcg cctcgaggat ctccggcatc atccggtagc
      181 ggctctcgac ccactgatac gggtcggtgt tcgtccccag cgccaccagc tcgcgcttcc
      241 aacccggccg cgccagctcc gcgcgcagca gctccggcac gttcaccttg accacgatct
      301 cccgctcgaa gtcccgccct gcgtccatgt cgaggtaggt gtgcgtgcgc cgggcgaagc
      361 agaaaacaca ggcatgggtg cagcctcgga acgggttgat cgtccagctg aagccgtagc
      421 ggccgccggg cacgtggttg agggccgact tggcgcggac ctcgtggaag ttgatcccca
      481 tcgcctcggg cacgtcgaag gtgcgggtca cggtgccgtc gccgacaccg gggatgcgga
      541 ggtcgttctc gactttttgt tcgtcccatc tcatacgaac gtatgttcgt atacgggtcg
      601 gacgcgctcg cgacgtcggg ccgcccctgg gcggccgcat gcaacgagcg gcgcggcgaa
      661 gctagaggct ggagccggtc tcttcgcgct tgtaggtggc gagctggtcg agcgctttcg
      721 cccgcacttt cttgcgcagc ggcgggatca gggcgacgaa gccgccggcg ccgccgagcg
      781 cctgcttgca ccagctgcgg aagtcgaagt cgtcgacgtg gtcggcgatc aggccgtccg
      841 gggtgaagcg gaagcgggcg cggacgtcgt tgacgaccgg gcggccggta gagaacgtgt
      901 agcgggcgat ccagtgggcg gtgccggcct gctcgtcggc ctcgtgctcg tgcagctcga
      961 tctcgaggtc ggtggcgcgg ccggtcagca tccgccacat cgcgccgatg tcgtcgccgt
     1021 gcaggtcgcc gaaggcgggg tcgcagaagt gggcgtcggg cgcgtagcaa gcggtcatcg
     1081 ccgctccgtt gcactctccg aacgcggcgt acagacgctc gatcgtcgcc tggttctcgg
     1141 cggcccccat gggaccagcc tacggcagtt tgccggcgat cacgtaacgc agcacgtcaa
     1201 ccacctgctc ggccgtgcgc gcggtcgcct gggcggcgcg gtcgacctcc ttgagcgcgt
     1261 ggtcgagctc accgtcgtgc agggtgatga tcggcttgcc gagcgccgcc gcgtagccag
     1321 cgtcgaaggc cgcattccac tggcggtatt tgtcgccgaa gcggaccacg accaggtcgg
     1381 cggcgcggat cagcgtctgg gtgcggatcg cgttgacgcc ggcgcctttg tggtcgcgcc
     1441 agaacgcctc gccctcctcc cccagggtgc ccgtgccggc gtcgtcggaa gcggcgtggt
     1501 cggtgaccgg cgccgcgaag acgaccggca gcccggcctc gcgcacgccc gcttcgattc
     1561 gctcgcgcca gtccgagtgg atctcgcccg agagatagac gctccaggtc tccatcggcc
     1621 gaatcctacg cgctgctagg ctaattgacg tgaacgttag aaagaggttt ggtacggcgg
     1681 gggtcgccag cgaccacgag cactacaagt ggtgggcgct ctcctgcacc agcctcggga
     1741 tgctcctcgt cgcgaccaac tcggggacgt tgatcatcgc cctgcccgac ctcgagcggg
     1801 cgctcgacac cgacctgctc gaactggtct gggtgatcct cgcctacatg atctcctcga
     1861 ccgtgctggt gctcacggcc ggtcgcctct ccgacctctt cggccggaag accgccttcg
     1921 tcgccgcctt cctgatcttc gccgccgcct cgctcggcgc cggcttcgcc gccgacggca
     1981 cccagctgat cctctggcgg atcctgcagg gaatcggcgg cgccttcatc ttcgccaacg
     2041 gccccgccct ggtcaccgac gccttcccca aggagcagct cgggctggcg atggggacga
     2101 acacgatgat cgccgcgatc gggttggtga tcgggccggt gctcggcggc gccctggtcg
     2161 cgatctcctg ggagtgggtc ttctggttca acgtcccgct cgcgctggcc ggggcgctct
     2221 gggccggcct ggtcctgcgc gagctctccc gccgcgacac cgtccgcggc ctcgacctgc
     2281 tcggcacctc ggccttcgtc gtcggcctga ccgggctcgt ctacgggatc tcgcgcggcg
     2341 ggctcagtgg ttgggataac aacctcgtga tcggcagcct gatcgccgcc gccgtcctgc
     2401 tgccgctgtt cgtgctgatc gagcaccgcg gcaaggcgcc gatgctcgac ctgacgatct
     2461 tccagaaccg cctcttcgcg gcggccagcg ccgcgacctt catcaacggc ctctcgcgct
     2521 tcgccctgat gttcatcttc gtcttctact tccagggcgc ccagggcgac gacccgatca
     2581 tcgccgggat caagctggcg ccgatggcgg tcgggatgct ggttgcctcg ccgctggccg
     2641 ggatctgggc cgaccgccac ggctcccggg tcctggcggc gttggggatg gtggtcacgg
     2701 cgatcggcct ggcgctgatg acgaccctgc aggaggacac cgcctactgg caggcgctga
     2761 tctggctcgg cgtcgtcggg atcggctccg gcatgttcat gtcgccgaac acggcggcaa
     2821 tgatgggcac ggtgccgacc gagcggcgcg ggatcgccgc cggcgcacgg acgatgctgc
     2881 agaacaccgg cgccgtgatc tcgatcgcct tcgtgctcgc gatcatcacc gcggcggtgc
     2941 cgaaaccggt cctcttcaag atcttctccg gcctcgcctc cggcctcacc gacgcccagc
     3001 tcgacccctt catcgccaac atgcacgagg cgctctgggt gctcgccgtg atctccgtgg
     3061 tcggcgccgg cgtctccctg ctgcgcccgc gcgagcagcg cgcccccgac agcgaccgcg
     3121 ggctgacgat cgacgaggcg atccgcgagg agatcgaggc gacgccatga gcccctcccc
     3181 cgcccgcggc gagctgcgga tcggcgaggt cgcccgcctc gccgggacca ccccgcgcac
     3241 gatccgctac tacgaggaga tcgggctgct cccgatcagc ggcggccgcg agcccggcgc
     3301 ccaccgctcc tacgccgaga gcgacgtcga acgactgacc gagctgctgc ggctgaaaga
     3361 cctgctcggg ctctcgctcg aggagctgaa gacactggcc gaggccgacg aggcgcgggc
     3421 ggcgctgcgg cgcgagtggc agggcggcgt cgccgacccg gcccggcgcg agcagatcct
     3481 cgaggagtcg ctcggccaca tcggccgcca gctcgacctc gtgcgccggc gccgcgacga
     3541 gatcgccgcg ctggaatcgg agctgaccgc caaacggcgg cgcgtccgcg aactgctgcg
     3601 cgagctgcct gcgccgacgg cctaagcggc ggcgggagca cccgcggtct gctccgcgaa
     3661 gtgcttgctc agcatcgtca cctggccctg gtagttggag ccgaggtcgg cgccgtagag
     3721 acgctcggcc gggccctcca tccactccag ggcgagcttg cagaccggct ggcggtgctc
     3781 gaccatgaac gagacgtcac gggcgcgcac ctcgagcgcg gcgcggctgc cgggccggtt
     3841 gccggcgggg tcgaagccga agccggggtc gaagaagccc gcgtagtg
//
LOCUS       DASRIG010000541        10132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1559490, whole genome shotgun sequence.
ACCESSION   DASRIG010000541 DASRIG010000000
VERSION     DASRIG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559490"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFB52_14450"
     CDS             complement(<1..744)
                     /locus_tag="VFB52_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07587.1"
                     /translation="MAPSFGSYIGGCLAVIGIAAALGLGGYWLRRWIVPEFSGALARL
                     ADATIAVALLILALEVLGTIGILTLGWTILGCIATGLGAAALGHRMAPPASREVEAPA
                     VRKVALWIAIGVASFTVAEWTVPTFLSLDQGMFGGDTTWYHMPFSAAIAQEGSTWPLH
                     FTDPLRLAAWFYPASHELVNAAGIVIFKNDWLAPIQNMFWIAIALLAAYCVGRPYKVG
                     PATMIAAAIVLDSGVMIETQPGEARNDIMG"
     gene            complement(773..1450)
                     /gene="msrA"
                     /locus_tag="VFB52_14455"
     CDS             complement(773..1450)
                     /gene="msrA"
                     /locus_tag="VFB52_14455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZO07588.1"
                     /translation="MTRLGKLMEMLNPEKTKMVSAEQALPGRSEPAFEVPATHAVLGT
                     PLKPPFPDGIETAYFALGCFWGAERLFWELDGVYTTAVGYQNGTTPNPTYEEVCSGMT
                     GHAEAVLVAFDPTKVSYADLLKVFFEEHDPTQGMRQGNDVGTQYRSGIYFLNDAQKAT
                     AEMVRDSYEEQLKASGRESVTTEIEPAGPFYYAEDYHQQYLHKVPNGYCGLKGTGVTC
                     AIPTAAS"
     gene            1563..2540
                     /locus_tag="VFB52_14460"
     CDS             1563..2540
                     /locus_tag="VFB52_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZO07589.1"
                     /translation="MRRLTCGAATLLAVLAMAGGTASAAPVTVGSPLTQAFAPTAFSE
                     LAIVANTVLAEPSARATSPVAGVVVEWHVLGAAGGPFTLRVLRPQPGGFYLAAGTSAP
                     AAPTGLGLQTFPALVPIKAGDLVGLEPGQGGDKIGTVSSTAGAAFAAFKLPFPDGSTA
                     FPLGSSNREVAFNAVVKPAPVVTAVSPKSGSFKGGTKVTITGTDLSGASAVSFGGAPA
                     KSFTVDSETQITAVTAAAKKPDAAAVAVTTVAGTATVAGFKLTACVVPNLKSKKLEAA
                     KKKLKSAGCKVGKVTKTNGVSAKTGKVTKQGKKAGNKLAPGTKINVTLG"
     gene            2557..3537
                     /locus_tag="VFB52_14465"
     CDS             2557..3537
                     /locus_tag="VFB52_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZO07590.1"
                     /translation="MKRIGLGTASLLAVLAMSGASASAAPVTIGNPLVSPATPSSGSG
                     ITASLLNLVVAEPGAQATSPVTGAIVRWSLAASEGGPFRLRVLRPAGGISYTAVGTSA
                     SAFAASTAAVETFPTALPIQAGDTIALDIVKGAKLGITPNPASVIGAIEPILVEGTTD
                     ALDVSTPGAEFAFNAVVQPAPTVTKVTPATGSFKGGTKVKITGTDFTGASAVSFGGAP
                     AKSFTVKSETQITAVIAAAKKPGPAAIAVTTIAGTATAKGFKLTACKVPKLKTKTLQA
                     AKKALKKGGCKLGKVTKTSGVTTKTGKVTKQGKPAGKKLAPGTKVSVTLG"
     gene            complement(3547..4479)
                     /locus_tag="VFB52_14470"
     CDS             complement(3547..4479)
                     /locus_tag="VFB52_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZO07591.1"
                     /translation="MSEKWTAADIPDQSGRTAVVTGANSGLGLATAEGLAAAGAEVVM
                     ACRNTAKADAGAAAILERHPGASLRVEPLDLADLASVRDFAARFAATGSGLDLLINNA
                     GVMASPRRETADGFELQLGTNHLGHFALTGLLLGRMEGREDARVVTVSSTAHKFGRIN
                     FDNLQGERRYFRWWAYGQSKLANLLFMLELDRRLRAAGSPVKSLAAHPGYADTNLQTA
                     APPLIDRAVFAVSNRLLAQNAEMGALPQLYAATRPNLDGGLFIGPDGFEEQRGYPKVV
                     GVARRGRDEADAARLWEVSEQLTGVRFELAGTPA"
     gene            4569..5213
                     /locus_tag="VFB52_14475"
     CDS             4569..5213
                     /locus_tag="VFB52_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07592.1"
                     /translation="MAKSSAPARKARGTYHHGDLPAALVAATFELIGERGVNGFSVAE
                     AARRTGVSISAPYRHFADRDELLAACATAALGAIREDFEAALAAHDSPADQLATAISA
                     YVRFAAEQRPMFESIFATGLDKTRYPALEEAGAAALSALSAPAVALAPSDDPAAAEVL
                     IQALTSLAQGHATMLLDGYFGEPPEAIDAAAERAAGATRALLRGRSLLYSQPAR"
     gene            5223..5495
                     /locus_tag="VFB52_14480"
     CDS             5223..5495
                     /locus_tag="VFB52_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07593.1"
                     /translation="MGCLLALFAFISPRLALFVMFLFTDLLSRAFDSWVLPLLGFFLL
                     PWTTLAYAAMWNIGDRGVSGFDWFIVILAFVVDLGAYASRDRARRT"
     gene            complement(5506..5784)
                     /locus_tag="VFB52_14485"
     CDS             complement(5506..5784)
                     /locus_tag="VFB52_14485"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07594.1"
                     /translation="MSEPAPGVSPDQIERRTERAVFETDGHTIVGDVTLPPAGYQSRF
                     SDALNRGDLEFLSLTNVEITSLTDGKVIERPFIVLSKAQIRLAYPVRA"
     gene            6185..6553
                     /locus_tag="VFB52_14490"
     CDS             6185..6553
                     /locus_tag="VFB52_14490"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HZO07595.1"
                     /translation="MEVPGLGDTEAELWLAVERGFPADGPSYVRLIGGLIAEQERHPE
                     LIEAFRERILLPRRALARELVERGQGRGDVRADVDPEAAVDSLAGPLLARVFAGVDTG
                     PDWRRKAFEIWWHSIEQRNH"
     gene            6553..6996
                     /locus_tag="VFB52_14495"
     CDS             6553..6996
                     /locus_tag="VFB52_14495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZO07596.1"
                     /translation="MIEFSVETEIARSPADVFAYVTDPSKLATWQRNTVSVSQEGDGP
                     IGVGTRLHEVHRAGGRELESVVEVSEYEPDRLFALKMIEGPLPLDARIEFEPAGGGTH
                     FTFTGSGEPSGMMRLAGPLLVRTLKRQFAEHCEHLKEVLEADSPG"
     gene            7025..7582
                     /locus_tag="VFB52_14500"
     CDS             7025..7582
                     /locus_tag="VFB52_14500"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HZO07597.1"
                     /translation="MELRDWIEEARAAGLAQPASVAFVTVGADGAPSARTVNLKRIEP
                     GALVFSSAMWTRKAREIAANPRVSLLFHWPSLGRQVHVAGEAAWAERELAEELFDERD
                     LHHQLQTVVSRQGEPIEDLDPLRDRLNHLVSVQETPPRCPEDWGALRVVPAVVELWEE
                     APDRLHQRRLCERDGEGWAVTLLSP"
     gene            7582..8004
                     /locus_tag="VFB52_14505"
     CDS             7582..8004
                     /locus_tag="VFB52_14505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZO07598.1"
                     /translation="MAAAIRTAAEADLGATLDLWRAAEAQPTATDDEAGLRTLLAHDP
                     RALLVAEQGGELVGSLIVGWDGWRGSLYRLAVHPSQRRQGLGTALIRAGEEQLVSQGA
                     RRFTAIVADDEAPAMAFWAALGYERQAGRARFVRTLPA"
     gene            complement(8024..9034)
                     /locus_tag="VFB52_14510"
     CDS             complement(8024..9034)
                     /locus_tag="VFB52_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07599.1"
                     /translation="MHTQSRTAPSRSITSFKALVAALLALAALAGAFGAEAQAAGGPG
                     RIVFVAGKAERCKGDACNRFDEVRSISPHGRGGQRLALISSVSEIASTERGTLAVVSR
                     NVAGGGANSEVFTQIYMLTPGGKRTAVFDDRIEGFNPRGLGISGNGRLLVVAARYEAE
                     TGTGRSKLFVVRPDGSGMRQLTDGRDNDEMPALSPDGKRVVFSRTVDGKRNPDLYVVP
                     VSGGEPVQLTDSGLAEINPVWSPDGRRIAFGQANMRANRGTIAVARADGSGVRTLTAT
                     GSEWPDPDFSPNGRSLVFVGELPGRGYDTALYTVRTSGGGRTIASRAYEYPELAQWTL
                     RP"
     gene            complement(9149..9222)
                     /locus_tag="VFB52_14515"
     tRNA            complement(9149..9222)
                     /locus_tag="VFB52_14515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9186..9188),aa:Pro,seq:tgg)
     gene            complement(9270..>10132)
                     /locus_tag="VFB52_14520"
     CDS             complement(9270..>10132)
                     /locus_tag="VFB52_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07600.1"
                     /translation="PPAGLQPAPAVLVFNDRIASHAGALADSPELVRLTQPIDIERFR
                     PRDAARPRARRVLLLSNYLDGARLQMLEGVCDELGLELARIGATGTPVASPQEAIADA
                     DIVVGYGRSVLEGMAMGRAAYVWDYAGGDGWVTPESYAAHEADGFSGAGSEAVIDAAR
                     LRADLAAYSPELGEFGWDLVRLHHSAAKHAERLVDVMEAASAPRPEAAASELARLVRL
                     RSRAEIRVDGLETENKRMREALEAQHQRAEALEAQLGTVLRSRSWRLLELPRRLAAKL
                     RERLLKGGPQ"
BASE COUNT         1514 a         3665 c         3492 g         1461 t
ORIGIN      
        1 ccccatgatg tcgttgcgcg cctcgcccgg ctgggtttcg atcatcaccc cggagtcgag
       61 gacgatcgcg gcggcgatca tcgtcgccgg cccgaccttg taggggcggc cgacgcagta
      121 ggcggcgagc agcgcgatcg cgatccagaa catgttctgg atcggcgcca gccagtcgtt
      181 cttgaagatc acgatcccgg ccgcgttgac gagctcgtgg ctggccgggt agaaccaggc
      241 ggcgagccgc agcgggtcgg tgaagtgcag cggccaggtc gagccctcct gggcgatcgc
      301 cgcggagaac ggcatgtggt accaggtggt gtcgccgccg aacatgccct ggtcgaggct
      361 gaggaaggtc ggcaccgtcc actcggcaac cgtgaacgaa gcgaccccga tcgcgatcca
      421 gagcgcgact ttgcggaccg ccggcgcctc gacttcgcgg ctggccggcg gcgccatccg
      481 gtggccgagt gcggcggcgc cgaggccggt agcgatgcag ccgaggatcg tccagccgag
      541 cgtgaggatc ccgatcgtgc cgagcacctc gagcgccagg atcagcagcg cgaccgcgat
      601 cgtcgcgtcg gcaagccgcg ccagggcgcc ggagaattcg ggcacgatcc agcgccgcag
      661 ccagtagcca ccgagcccga gcgcggcggc gatgccgatt acggccaggc agccgcctat
      721 gtaagaaccg aaagagggcg ccacgagggc gggatgctat cccgcccgcc ccctatgaag
      781 ctgccgtggg gatcgcgcag gtgacgccgg tccctttcag gccgcagtag ccgttcggca
      841 ccttgtgcag gtactgctgg tggtagtcct cggcgtagta gaagggcccg gcgggctcga
      901 tctcggtcgt gaccgactcg cgcccggacg ccttcagctg ctcctcgtag ctgtcgcgga
      961 ccatctccgc ggtcgctttc tgggcgtcat ttaagaagta gatcccagag cggtactggg
     1021 tcccgacgtc gttgccctgg cgcatgccct gggtcgggtc gtgctcctcg aagaacacct
     1081 tcagcaggtc cgcgtaggag accttggtcg ggtcgaaggc gaccaggacc gcctcggcgt
     1141 gcccggtcat ccccgagcag acctcctcgt aggtggggtt gggcgtggtc ccgttctggt
     1201 agccgaccgc agtcgtgtag acgccgtcca gctcccagaa caaccgctcc gcgccccaga
     1261 aacaaccgag cgcgaagtag gcggtctcga tcccgtccgg gaacggcggc ttcagcgggg
     1321 taccgagcac ggcgtgggtc gcgggcacct caaacgcggg ctcgctacgg ccgggcaggg
     1381 cctgctcggc gctcaccatc ttggtcttct cgggattcag catctccatc aactttccga
     1441 gtctcgtcat ctgtttcctc ctgtttctct acctacaact ttacgcctcg cgtctagtcc
     1501 cctcgcccgc ctggagctaa ggttgggcgc ggttcaagcg agggacacga gaaggagcgg
     1561 cgatgcgacg gttgacctgc ggtgcggcga ccctgctggc tgtgctggcg atggcgggcg
     1621 ggacggcgag cgcggctccc gtcacggtcg gcagcccgct cacccaggcc ttcgccccga
     1681 ccgctttcag cgagctcgcg atcgtcgcca acaccgtcct tgccgaaccg agcgcccgcg
     1741 ccacctcgcc ggtcgccggc gtggtggtcg agtggcacgt gctcggcgcc gcgggcgggc
     1801 cgttcacgct ccgcgttctg cgcccgcagc cgggcggctt ctacctcgcc gccggcacca
     1861 gcgcccccgc cgccccaacc ggcctgggac tgcagacctt cccagcgctt gtgccgatca
     1921 aagcgggcga cctggtcggc ctcgaaccgg ggcagggagg cgacaaaatc ggcacggtct
     1981 caagcacggc cggagccgcc ttcgccgcgt tcaaactccc gttcccggac ggctcgaccg
     2041 ctttcccgct cggcagcagc aaccgcgagg tcgccttcaa cgccgtggtc aagccggcgc
     2101 ctgtcgtgac cgccgtgtcg cccaagtccg gcagcttcaa gggcggcacc aaggtgacga
     2161 tcaccggcac cgacctcagc ggggcgagcg cggtcagctt cggcggcgcg ccggcgaaga
     2221 gcttcacggt cgactcggaa acccagatca ccgccgttac cgcagcggcg aagaaacccg
     2281 acgccgccgc ggtcgcggtg accacggttg ccgggaccgc gaccgttgcg ggcttcaagc
     2341 tgaccgcgtg cgtcgtcccg aacctgaaga gcaagaagct ggaagcggcg aagaagaaac
     2401 tcaagagcgc cggctgcaag gtcggcaagg tgaccaagac gaacggcgtg tccgccaaaa
     2461 ccggcaaggt caccaagcaa ggcaagaaag ccgggaacaa gctcgcaccc gggaccaaaa
     2521 tcaacgtcac gctgggttag cgaaagggag tctcggatga agcggatcgg tctgggtacg
     2581 gcgagcctgc tcgccgtgtt ggcaatgagc ggcgccagcg cgagcgccgc gccggtgacg
     2641 atcggcaacc cgttggtcag cccggcgacg ccgtccagcg gctcgggcat caccgcgagc
     2701 ctgctcaacc tcgtggtcgc cgagccggga gcgcaagcga cctcgccggt gaccggcgcg
     2761 atcgtccgct ggagcctggc cgcctccgaa ggcgggccgt tccgcctgcg ggtcctgcgc
     2821 cccgccggcg gcatctccta caccgccgtc ggcaccagcg ccagcgcctt cgcggccagc
     2881 acggccgcgg ttgaaacctt ccccaccgcg ctaccgatcc aggccggcga cacgatcgcg
     2941 ctcgacatcg tcaaaggggc gaaactcggg atcacgccga acccggcctc ggttatcggc
     3001 gcgatcgagc cgatcctggt cgaagggacg accgacgccc tcgacgtctc cacccccggc
     3061 gccgagttcg ccttcaacgc cgtggttcag ccggcgccga cggtgaccaa ggtgacgccg
     3121 gccaccggca gtttcaaagg cgggaccaag gtgaagatca ccggcaccga cttcaccggc
     3181 gcgagcgcgg tcagcttcgg cggcgcaccg gccaagagct tcacggtcaa atccgaaact
     3241 cagatcaccg cggtcatcgc cgcggcgaaa aagcccggcc ctgccgcgat cgcggtgacc
     3301 accatcgccg gaaccgcgac cgccaaaggc ttcaagctga ccgcttgcaa ggtcccgaag
     3361 ctgaagacga agacgctgca ggccgcgaag aaggcgctga agaaaggtgg ctgcaagctc
     3421 ggcaaagtga ccaagaccag cggcgtcacc acgaaaacgg gcaaggtcac caagcagggc
     3481 aagccggccg gcaagaagct cgcgccgggc accaaggtca gcgtgacgct cggctgagcg
     3541 ccgagctcag gcgggcgtgc cggcgagctc gaagcgcacg ccggtcagct gttcggagac
     3601 ctcccagagg cgcgccgcgt cggcctcgtc gcgaccgcgg cgggcgaccc cgaccacctt
     3661 cgggtagccg cgctgctcct cgaacccgtc ggggccgatg aagaggccgc cgtcgaggtt
     3721 ggggcgggtc gcggcgtaga gctgtggcag cgcgcccatc tccgcgttct gggcgagcag
     3781 acggttgctg accgcgaaca cggcgcggtc gatcagcggc ggcgcggcgg tctgcaggtt
     3841 ggtgtcggcg tagcccgggt gggcagcgag gctcttgacc ggggagccgg cggcgcgcag
     3901 gcgccggtcc agctcgagca tgaacagcaa gttggcgagc ttcgactggc cgtaggccca
     3961 ccagcggaag tagcgccgct caccctgcag gttgtcgaag ttgatgcggc cgaatttgtg
     4021 cgcggtggag ctgaccgtga ccacgcgggc gtcctcgcgg ccctccatgc ggccgagcag
     4081 caggccggtc agtgcgaaat ggccgaggtg gttggtgccg agctgcagct cgaagccgtc
     4141 ggcggtctca cgccgcggcg aggccatcac gccggcgttg ttgatcagca ggtcgaggcc
     4201 gctgcccgtg gccgcgaagc gcgcggcgaa gtcgcgcacc gaggcgaggt cggcgaggtc
     4261 gagcggctcg acccgcaggc tcgcgccggg gtggcgctcc aagatcgcgg cggcgccggc
     4321 atcggccttc gccgtgttgc ggcatgccat caccacctcc gccccggccg cggccaggcc
     4381 ctccgcggtc gccaggccga gcccgctgtt ggcgccggta accaccgccg tgcgcccgct
     4441 ctggtcggga atgtccgccg ccgtccactt ctcgctcatc gctcaacctc cgcttgccct
     4501 ggtgactggt tcgtcccatc agtgtatgtg aatctctctt actttgtcgt gcgactagag
     4561 ttcgggcaat ggcgaagtcc tcggcaccgg cccgcaaagc gcgcggcacc taccaccacg
     4621 gcgacctgcc cgcggcgctc gtcgccgcca ccttcgagct gatcggcgag cgcggcgtga
     4681 acgggttctc ggtcgccgaa gcggcgcggc gcaccggggt cagcatctcc gccccctacc
     4741 gccacttcgc cgaccgcgac gagctgctcg ccgcctgcgc caccgccgcg ctgggagcga
     4801 tccgggagga cttcgaggcc gcgctcgccg cccacgactc ccccgccgac cagctcgcca
     4861 ccgcgatctc cgcctacgtc cgcttcgccg ccgagcagcg gccgatgttc gaatcgatct
     4921 tcgccaccgg cctcgacaag acccgttacc cggcgctcga ggaggccggc gcggcggcgc
     4981 tctcggcgct ctcggccccc gccgtcgcgc tcgccccgag cgacgacccg gccgccgccg
     5041 aggtcctgat ccaggccctg accagcctcg cccagggcca cgcgacgatg ctgctcgacg
     5101 gctacttcgg cgagcccccg gaggcgatcg acgccgccgc cgagcgcgcc gccggcgcga
     5161 cccgcgccct gttgcgcggc cgcagcctcc tctacagcca gcccgcgcgg taggttccgc
     5221 agatgggttg cctgctcgct cttttcgcgt tcatctcgcc gcggctggcg ttgttcgtga
     5281 tgttcctctt caccgacctg ctgagccgcg ccttcgacag ctgggtcctg ccgctgctcg
     5341 gcttcttcct gctgccctgg acgacgctcg cctacgcggc gatgtggaac atcggcgacc
     5401 gcggggtcag cgggttcgac tggttcatcg tgatcctcgc cttcgtcgtc gacctcggcg
     5461 cctacgcgag ccgcgaccgc gcgagacgca cctgagccgg gaccgctagg cgcggaccgg
     5521 gtaggcgagc cggatctgcg ccttggagag gacgatgaac ggccgttcga tcactttgcc
     5581 gtcggtcagc gaggtgattt cgacgttggt cagcgagagg aactcgaggt cgccgcggtt
     5641 gagcgcgtcc gagaagcggc tctggtagcc cgccggcggc agggtgacgt cgccgacgat
     5701 cgtgtgtccg tcggtctcga agactgcgcg ctcggtgcgg cgctcgatct ggtcgggcga
     5761 aacgcccggt gctggttcgc tcattccgtt catgttcatc ggtatgaaat gtcggaaacg
     5821 cgcctgaatt ccgccatcga acaaacgtcc aggtggcgtt tagccccatt ccagagccat
     5881 atgcgggcgg caattttcgt accgaagtgt tttccgatca gcgtttctag ctgctttgac
     5941 cgctttaacc tttgacataa cgtaacgcca gcgttacgtt attgcgatcg cgcgcacccg
     6001 cgacccccag accgacgagg caattcacgc cgccactctc gctctgctca gccagcgcgg
     6061 cttcgccgcg acgacgatcg aggcgatcgc cgccacagcc ggcgtcggca agccggcgat
     6121 ctaccgccgc ttccccgaca aggcgaccct ggtcgtcgcg acgatcgccg cgcagctgcc
     6181 gcagatggag gtgccagggc tcggcgacac cgaagccgag ctgtggctcg cggtcgagcg
     6241 cggcttcccc gctgacggcc cctcctacgt gcgcctgatc ggcgggctga tcgccgagca
     6301 ggaacgccac ccggagctga tcgaggcgtt ccgcgagcgc atcctgctgc cgcgccgcgc
     6361 gctcgcccgc gaactagtcg aacgcggcca ggggcgtggc gacgtacgcg ccgacgtcga
     6421 ccccgaagcc gcggtcgact cgctcgccgg cccactgctc gcccgcgtct tcgccggcgt
     6481 cgacaccggg cccgactggc gtcgcaaagc cttcgagatc tggtggcact caatcgagca
     6541 aaggaaccat tgatgatcga attcagcgtc gaaaccgaga tcgcccgcag cccggccgac
     6601 gtcttcgcct acgtgaccga tccgtcgaag ctcgccacct ggcagcgcaa caccgtctcg
     6661 gtcagccagg agggcgacgg cccgatcggc gtcgggaccc gcctacacga ggtccaccgc
     6721 gccggcggca gagagctcga gtcggtggtc gaggtctccg agtacgagcc cgaccgcctc
     6781 ttcgcgctga agatgatcga aggcccgctg ccgctcgacg cccggatcga attcgagccc
     6841 gccggcggcg gcacccactt caccttcacc ggcagcggcg agcccagcgg gatgatgcgc
     6901 ctcgccggcc cgctgctcgt ccgcaccctg aagcgccagt tcgccgagca ctgcgagcac
     6961 ctcaaagagg tgctcgaggc ggactctccc ggctgacacc taaactgacc cgatcgaccc
     7021 tctgatcgag ttgcgggact ggatcgagga ggcacgagcg gcggggctcg cccagccggc
     7081 ctccgtcgcc ttcgtgaccg ttggcgccga cggcgccccc tcggcgcgca ccgtcaacct
     7141 caagcggatc gagcccgggg ccctcgtctt cagctcggcg atgtggacgc gcaaggcgcg
     7201 cgagatcgct gccaacccgc gcgtctccct gctcttccac tggccctctc tgggccgcca
     7261 ggtccacgtc gccggcgagg ccgcctgggc cgagcgcgaa ctcgccgagg agctcttcga
     7321 cgagcgcgac ctccaccacc agctgcagac cgtcgtctcc cgccagggcg agccgatcga
     7381 agacctcgac cccctgcgcg accggctcaa ccacctggtc agcgtgcagg agaccccgcc
     7441 gcgctgcccc gaggactggg gtgccctgcg cgtggtccca gccgtcgtcg agctctggga
     7501 ggaagccccc gaccgcctcc accagcgccg cctctgcgag cgcgacggcg agggctgggc
     7561 cgtgaccctg ctctcgccct gatggccgcc gcgatccgca ccgccgccga ggccgacctc
     7621 ggcgcgaccc tcgacctctg gcgggcggcc gaggcacagc cgaccgcgac cgacgacgag
     7681 gccggcctgc gcaccctgct cgcccacgac ccgcgggcgc tgctggtcgc cgagcagggc
     7741 ggcgagctgg tcggctcgct gatcgtcggc tgggacggct ggcgcggcag cctctaccgc
     7801 ctcgccgtcc accccagcca gcgccgccag ggcctcggca ccgccctcat ccgtgccggc
     7861 gaagagcagc tcgtctccca gggtgcccgc cgcttcaccg cgatcgtcgc cgacgacgaa
     7921 gcgcccgcga tggcgttctg ggccgcgctc ggctacgaac gccaggccgg gagagcgcgg
     7981 ttcgtccgca ccctcccggc ctgatcgctc ccggcccgag tccctacggg cgcagcgtcc
     8041 actgcgccag ctccgggtac tcgtaagcgc gcgaggcgat cgtgcgacca ccgccgctgg
     8101 tgcgcaccgt gtagagcgcg gtgtcatagc cgcggcccgg cagctcaccg acgaagacca
     8161 ggctgcggcc gttcggcgag aagtcggggt cgggccattc gctgccggtc gcggtcagcg
     8221 tgcgcacgcc gctgccgtcg gcacgagcga cggcgatcgt gccgcggttg gcgcgcatgt
     8281 tggcctgacc gaaggcgatc cggcggccgt cggggctcca gaccgggttg atctcggcga
     8341 ggccgctgtc ggtcagctgc accggctcgc cgccgctgac cgggaccacg tagaggtccg
     8401 ggttgcgctt gccgtcgacc gtgcgggaga agacgacgcg cttaccgtcg ggcgagagcg
     8461 ccggcatctc gtcgttgtcg cgaccgtcgg tgagctggcg catgccgctg ccgtcggggc
     8521 gcacgacgaa gagcttcgaa cggccggtcc cggtctccgc ttcgtacctg gccgcgacaa
     8581 ccagcagcct gccgttgccg gagatcccga ggcccctcgg gttgaagccc tcgatccggt
     8641 cgtcgaagac cgccgtgcgc ttgccgcccg gggtgagcat gtagatctgg gtgaagacct
     8701 cggagttggc gccgccgccg gcgacgttgc gcgagaccac ggcgagcgtg ccgcgctcgg
     8761 tcgaggcgat ctcggagacc gagctgatca gcgccagccg ctgcccgccg cgcccgtgcg
     8821 ggctgatcga gcggacttcg tcgaagcgat tgcaggcatc gcccttgcag cgctcggcct
     8881 tgccggcgac gaagacgatc cggccgggac cgccggccgc ctgggcctcg gcgccgaagg
     8941 caccggcgag cgccgccagc gccagcagcg ccgcgacaag ggccttgaac gaagtgatgg
     9001 agcgggacgg agcggtgcgg gactgggtgt gcatcgaggg cctcctgagc ctgggattga
     9061 cgatgcaccg atcgtcgccc ggccaagcgc ctaggcccag cacccctggg gtggaatttt
     9121 ctcacccctc gaggtcgcgc ttctgtagtc ggggcgcccg gattcgaacc ggggacctcc
     9181 gccacccaaa ggcggcgcgc taccaggctg cgccacgccc cgaccgcgcc gattgtagtt
     9241 cggcgccgga gattcgctct acgggaccat cattggggac ctcccttcaa caggcgctcg
     9301 cgcagcttcg cggcgagtcg tcgtggcaac tcgagaaggc gccaggagcg cgagcggagc
     9361 accgtgccga gctgggcttc gagcgcctcg gcgcgctggt gctgggcctc cagcgcctcg
     9421 cgcatccgtt tgttctcggt ttcgaggccg tcgacccgga tctcggcgcg cgagcgcagg
     9481 cgcaccaggc gggccagctc ggaggcggcc gcctcgggcc gcggggcgct cgccgcctcc
     9541 atcacgtcga ccaggcgctc ggcgtgcttg gcggccgagt ggtgcaagcg caccaggtcc
     9601 cagccgaact cgcccagctc ggggctgtag gcggcgaggt cggcgcgcag gcgcgcggcg
     9661 tcgatcaccg cctcgctgcc ggcaccgctg aaaccgtcgg cctcgtgcgc cgcgtagctc
     9721 tccggcgtca cccagccgtc gccgccggcg tagtcccaga cgtaggcggc gcgccccatc
     9781 gccatcccct cgagcaccga gcggccgtag ccgacgacga tgtcggcgtc ggcgatcgcc
     9841 tcctggggcg aggcgaccgg tgtgccggtg gcgccgatcc gggccagctc caggccgagc
     9901 tcgtcgcaga ccccctcgag catctgcagg cgggcgccgt cgaggtagtt gctgagcagg
     9961 aggacgcggc gggcacgcgg ccgggcggcg tcgcgggggc gaaagcgttc gatgtcgatc
    10021 ggctgggtca ggcgcaccag ctcggggctg tcggcgagcg cgccggcgtg gctggcgatc
    10081 cgatcgttga agacgagcac ggccggcgcc ggctgcagcc cggcgggcgg ct
//
LOCUS       DASRIG010000542         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3356822, whole genome shotgun sequence.
ACCESSION   DASRIG010000542 DASRIG010000000
VERSION     DASRIG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356822"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFB52_14525"
     CDS             <1..574
                     /locus_tag="VFB52_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZO07601.1"
                     /translation="ISALLAQAGEIDGQLRLIVTRGGHRIAATEQIPPHADSVAVATV
                     TYATTGILDRVKSLSYAANMQATRIAKGRDADEAVLITAEGTVLEPPTSTVFWATPEG
                     RLRTPSLDLPILDSITRDRLVAALEVEQGSFPAEDLRGASEAFLASTTREVQPIFAVD
                     GTPLPAVPGPRTQEAQRAFTAVLERELGLS"
     gene            575..1345
                     /locus_tag="VFB52_14530"
     CDS             575..1345
                     /locus_tag="VFB52_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZO07602.1"
                     /translation="MALLTVSANEFRVGLVQFDSPETRNALSPQMKAELIAALRTLDA
                     DPEIRCIVVAGSEKIFAAGADIAVLASQPLDATSDEERAAFWRALTEIETPLIAAVSG
                     YALGGGCELALACDMIVADKDARFGLPEVTLGIIPGGGGTQRLTRAIGKQRAMEYVLT
                     GRQFDAEMALEWGLINAAVGRKSWLGAALDQARTVAERAPIATRLAKQAVLAAEAGGL
                     AAGLERERELFDRVMTTEDRVEGTTAFLEKREPDFKGR"
     gene            complement(1352..1654)
                     /locus_tag="VFB52_14535"
     CDS             complement(1352..1654)
                     /locus_tag="VFB52_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZO07603.1"
                     /translation="MYGSRTATDCTHLDSVQFLSLPDQVEGCEECLAAGMRWVHLRMC
                     QTCGHIGCCDNSIGKHATGHHQGTGHPIIRSAEPGEDWSWCYVDELMFRLGDDHRA"
     gene            complement(1718..1891)
                     /locus_tag="VFB52_14540"
     CDS             complement(1718..1891)
                     /locus_tag="VFB52_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07604.1"
                     /translation="MGERLNLYVPFVLAVLFPPVGLLLALVGFQEGDRDKGIRLLVIS
                     VLAAVVWAFLFLA"
     gene            complement(1918..2922)
                     /gene="trxB"
                     /locus_tag="VFB52_14545"
     CDS             complement(1918..2922)
                     /gene="trxB"
                     /locus_tag="VFB52_14545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZO07605.1"
                     /translation="MTESKKVENVIIIGGGPAGYTAALYTARANLEPLVFEGFQWGGQ
                     LQNTTDVENYPGYPEGIMGPEMMSQFRAQAERFGTRFVTDDVDRVELSDGGVQKVFLG
                     DEEYRAKTVILAMGAEPKRLGIPGEMELGGRGVSTCGVCDGAFFKDQKVVVIGGGDSA
                     MEDAIFISKFAKDLTVVNRRDEFRASKIMFERAREKDNISLKTPFVPEEFVQGENGKL
                     EKIRLRNAETGETEEIEATGAFVAIGHVPRSEVVKDAVETDEDGYIVVEPGSTRTNLA
                     GVFAVGDVVDHTYRQAVTAAGTGCMGALDAERFLRDSPPPVEAHWMPGGEPAEIATGS
                     "
     gene            complement(2985..>3582)
                     /locus_tag="VFB52_14550"
     CDS             complement(2985..>3582)
                     /locus_tag="VFB52_14550"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZO07606.1"
                     /translation="IIPAAVAGLMLVLGVLIALIPTDLERRVLPHVHGEKARGWVRTA
                     AKIPSTVGEGFRFAMGLFAHPSSGGLAVLGAAGYWAASIGVLWASFHAFGVHVPLAVV
                     VQGFFLGMVANLFPLAPAGVGAVDAGMIGAFVLFGIPEETVFPAILIFRLVAFWMPIP
                     PGIVAFFQLRKTVHRWETEGLPIERGKGAKSDLEPSVG"
BASE COUNT          580 a         1266 c         1206 g          530 t
ORIGIN      
        1 gatctcggcg ctgctcgcgc aggcgggcga aatcgacggc cagctgcggc tgatcgtcac
       61 ccggggcggc caccgcatcg ccgcgacgga gcagatccca ccgcacgccg actcggtcgc
      121 ggtcgcaacc gtcacctacg cgaccaccgg catcctcgac cgggtcaagt cgctctccta
      181 cgccgccaac atgcaggcga cccggatcgc caaagggcgc gacgcggacg aggccgtgct
      241 gatcaccgcc gagggcacgg tgctggagcc gccgacctcg accgtcttct gggccacccc
      301 cgagggccgg ctccgcaccc cttcgctcga tctgccgatc ctcgactcga tcacccgcga
      361 ccgcctggtc gccgcgctcg aggtcgagca gggctccttc ccggccgagg acctgcgcgg
      421 cgcctccgag gccttcctcg cctcgaccac gcgcgaggtg cagccgatct tcgcggtcga
      481 cggcacgccc ttgcccgcgg tccccgggcc gcgcacgcag gaggcgcagc gggccttcac
      541 cgcggtgctc gagcgcgagc tggggctgag ctgagtggcc ttgctgacgg tctcggcgaa
      601 cgagttccgc gtcggtctcg tccagttcga cagccctgag acccgcaacg cgctctcgcc
      661 gcagatgaag gccgagctga tcgcggccct ccgcaccctc gacgccgacc cggagatccg
      721 ctgcatcgtg gtcgccggct cggagaagat cttcgccgcc ggggccgaca tcgccgtgct
      781 cgcctcacag ccgctcgacg ccacctccga cgaggagcgc gccgccttct ggcgtgccct
      841 cacggagatc gagacgccgc tgatcgccgc ggtctccggc tacgcactcg gcggcggctg
      901 cgagctggcg ctcgcctgcg acatgatcgt cgccgacaag gacgcgcgct tcggcctgcc
      961 cgaggtgacg ctcgggatca tccccggcgg cggtggcacc cagcgcctca cccgggcgat
     1021 cggcaagcag cgggcgatgg agtacgtgct gaccggccgc cagttcgacg ccgagatggc
     1081 gctcgagtgg ggcctgatca acgccgcggt cggccgcaag tcctggctgg gcgcggcact
     1141 cgaccaggcg cgcacggttg ccgagcgggc gccgatcgcg acccggctgg cgaagcaggc
     1201 ggtgctcgcc gccgaagcgg gtggcctcgc ggcaggcctg gagcgcgagc gcgagctgtt
     1261 cgaccgggtg atgacgaccg aggaccgggt cgagggcacg accgccttcc tcgagaagcg
     1321 cgagcccgac ttcaaaggcc gctgaccgcc gctaggcgcg gtggtcgtcg ccgagccgga
     1381 acatcagttc gtcgacgtag caccagctcc agtcctcgcc gggctcggcg gagcggatga
     1441 tcgggtgccc cgtgccctgg tggtggccgg tggcgtgttt gccgatcgag ttgtcgcagc
     1501 agccgatgtg cccacaggtt tggcacatgc gcagatggac ccagcgcatt ccagcggcta
     1561 ggcactcctc acaaccctca acctgatcgg ggagggagag gaactgaacc gagtcgagat
     1621 gagtgcaatc ggtggccgtc cgtgaaccat acatacgtcc ggcgaaaatt tcgctctaga
     1681 aagggaaaaa agaaacggag agtttcttta gtcccgctca ggccaaaaag agaaacgccc
     1741 agacgaccgc agccagcacg gagatcacga gcagcctgat ccccttgtcg cggtccccct
     1801 cctggaagcc gacgagggcg agcagcagcc cgaccggggg gaacagcacc gcgagcacga
     1861 acgggacgta gaggttgagg cgttcgccca ttcgcgaacc ctagccgtag tgagcgacta
     1921 agagccggtc gcgatctcgg ccggctcgcc gccgggcatc cagtgcgcct cgaccggcgg
     1981 cgggctgtcg cgcaggaagc gctcggcatc gagcgcgccc atgcagccgg tgccggcggc
     2041 ggtgacggcc tggcggtagg tgtggtcgac gacgtcgccg accgcgaaga caccggccag
     2101 gttggtccgg gtcgagccgg gctcgaccac gatgtagccg tcctcgtcgg tctcgacggc
     2161 gtctttgacg acctccgagc gcggcacgtg gccgatcgcg acgaaggcgc cggtcgcctc
     2221 gatctcctcg gtctcaccgg tctccgcgtt gcgcagccgg atcttctcga gcttgccgtt
     2281 ctcaccctgg acgaactcct ccggaacgaa cggggtcttg aggctgatgt tgtccttctc
     2341 cctggcccgc tcgaacatga tcttcgaggc gcggaactcg tcgcgccggt tgacgacggt
     2401 caggtctttg gcgaacttgg agatgaagat cgcgtcctcc atcgccgagt cgcctccgcc
     2461 gatcacgacc accttctggt ctttgaagaa ggcgccgtcg cagacgccgc aggtggagac
     2521 gccgcggccg ccgagctcca tctcgccggg gatcccgagc cgtttcggct ccgcccccat
     2581 cgccaggatc acggtcttgg cgcggtactc ctcgtcgccg aggaagacct tctgcacgcc
     2641 gccgtcggag agctcgacgc ggtcgacgtc gtcggtgacg aagcgggtcc cgaagcgctc
     2701 ggcctgagcg cggaactggc tcatcatctc cgggcccatg atcccctcgg ggtagcccgg
     2761 gtagttctcg acgtcggtcg tgttctgcag ctgaccgccc cactggaagc cctcgaagac
     2821 gagcggctcc aggttggcgc gcgcggtgta gagcgccgcc gtgtagccgg ccggcccccc
     2881 accgatgatg attacgttct cgaccttctt gctctcagtc atatctgcct ctggaagact
     2941 aatactttac tttttatagt actcccctgg aaggcggagg gcaatcagcc tacagagggc
     3001 tccaggtcgg atttcgcccc tttgccgcgc tcgatcggca gtccctctgt ttcccagcgg
     3061 tggaccgttt tgcgcagctg gaagaaggcg acgatccccg gcgggatcgg catccagaag
     3121 gcaaccaggc ggaagatcag gatcgccggg aagaccgtct cctcggggat gccgaagagc
     3181 acgaaggcgc cgatcatgcc cgcgtcgacc gcccccaccc cggccggggc gagcgggaag
     3241 aggttggcga ccatgccgag gaagaagccc tggacgacga ccgcgagcgg cacgtgcacg
     3301 ccgaaggcgt gaaaggaggc ccagagcacc ccgatgctgg ccgcccagta gccggccgcg
     3361 ccgagcaccg cgagcccgcc gctggagggg tgcgcgaaga ggcccatcgc gaagcggaac
     3421 ccctccccca cggtcgacgg gattttggcc gcggtgcgga cccagccccg cgccttctcc
     3481 ccgtgcacgt gcggcaacac ccggcgctcg aggtcggtcg ggatcagcgc gatcagcacc
     3541 ccgagcacca gcatcaagcc agccaccgcc gccgggatga tc
//
LOCUS       DASRIG010000543         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1813429, whole genome shotgun sequence.
ACCESSION   DASRIG010000543 DASRIG010000000
VERSION     DASRIG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813429"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..648
                     /locus_tag="VFB52_14555"
     CDS             49..648
                     /locus_tag="VFB52_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07607.1"
                     /translation="MGVTPWKLPLIVLAIAVPIAFSFYFGGPGVGVAVGALVAMVIVA
                     VAVRMRPREALGSPVSASAPKSLLVVAACAVEGAGPIAEIAAAAAPSHGALAAVLVLA
                     PARIGFLDRWASDVEGARREAQQRLVLTVASLAKAGVEAEARVGDEDVVQAVEDQLGS
                     FAATEVILVSAGPDEDPHAADAATELRRRLRPRFRWLTV"
     gene            complement(645..917)
                     /locus_tag="VFB52_14560"
     CDS             complement(645..917)
                     /locus_tag="VFB52_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07608.1"
                     /translation="MEGRGDNNWTTRAADLATESTVMRQVLTVHPVLLTVAELIRELA
                     GEDPGFAERDAIERAVRDLTGVGLLHGHGEFVLPTRAALHFSELDV"
     gene            complement(928..1263)
                     /locus_tag="VFB52_14565"
     CDS             complement(928..1263)
                     /locus_tag="VFB52_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07609.1"
                     /translation="MARKNRTDENALLSALGSPLRRRVLRRMRDVEMISPRELALEFE
                     LPLSTMAYHVRVLAGCGAITLVRVKPVRGAIKHYYRSSLEPPWARQIIDLEPWDGTRP
                     GEIPDSPPT"
     gene            complement(1339..>2229)
                     /locus_tag="VFB52_14570"
     CDS             complement(1339..>2229)
                     /locus_tag="VFB52_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZO07610.1"
                     /translation="LTESTLDHLGGYGGAQPVRIGNGAFDQRQNDVWGALLDSIYLHE
                     KALKGHGTKADRELIGYQVQAAIDAWPHPDQGIWESRGEPQHYVSSKLMLWVAVDRGA
                     RLAASVGRDDLAAEWRAKADEFKAEILERGVRDNVFRQHYDTDALDASTLLIPLLRFL
                     PPDDPRLHATVDAIAEELTEHGLVLRYKVQETDDGLHGKEGTFLICSFWLVSALSEIG
                     ECQRARTLCERLLEAAGWLDLFAEELDADSGRHLGNFPQAFTHLALINAVSHVIADEQ
                     RENGGVTAVFSEMGGIRAGN"
BASE COUNT          290 a          793 c          776 g          370 t
ORIGIN      
        1 agggcgccta gtccggcgaa tcgggacggc gctcgcgcct ggcaggctgt gggggtgacc
       61 ccgtggaagc tgcccctgat cgttctggcg atcgccgtcc cgatcgcgtt ttccttctat
      121 ttcggcggcc ctggagtcgg ggtcgcggtg ggtgccctgg tcgcgatggt gatcgtcgct
      181 gtcgcggtcc gaatgcggcc gcgcgaagca cttggcagcc cagtctcggc gagcgccccc
      241 aagagcttgc tcgtcgtcgc cgcctgcgcg gtcgaggggg ccgggccgat tgccgagatc
      301 gcggccgcgg cggcgccctc gcacggcgcc ctcgccgccg ttctcgttct cgccccagct
      361 cgcatcggct tcctcgaccg ctgggcctcg gacgtcgagg gcgcccgccg cgaggcccag
      421 cagcgcctcg tcctcaccgt cgcctcgctg gccaaggccg gggtcgaggc ggaggcgcgg
      481 gtcggcgacg aggacgtcgt gcaggcggtc gaagaccagc tcggcagctt cgcggcgacc
      541 gaagtgatcc tggtcagcgc cgggcccgac gaggacccgc acgccgccga cgcggccacc
      601 gagctgcgcc gccggctgcg cccgcgcttc cgctggctga ctgtctagac gtcgagctcg
      661 ctgaagtgca gcgccgcccg cgtcggcagc acgaactcgc cgtgcccgtg caggaggccg
      721 acgcctgtga ggtcgcgcac cgcgcgttcg atcgcgtcgc gctcggcgaa gccggggtcc
      781 tcgcccgcga gttcgcggat cagttcggcg accgtgagca gcaccgggtg gacggtcagc
      841 acctgcctca tcacggtcga ctcggtcgcc aggtccgccg ctctcgtcgt ccagttgttg
      901 tcgcccctgc cctccatgac tccccggcta agtcggcggg ctgtctggaa tctcccccgg
      961 ccgggtcccg tcccacggct cgaggtcgat gatctgccgc gcccagggcg gttccaggga
     1021 gctgcggtag tagtgcttga tcgccccacg caccggtttc acccgcacca gggtgatcgc
     1081 gccgcagccg gccagcaccc gcacgtggta ggccatcgtg ctcagcggta gctcgaactc
     1141 cagcgccagc tcccgcgggc tgatcatctc cacgtcgcgc atccgcctca gcacccggcg
     1201 ccgcagcgga ctgccgagcg ccgacaacag cgcgttttcg tcggtgcggt tcttcctcgc
     1261 catcccgcgc caatataaag caacagaaat gttttgtctg taaacctgtt gtccgcacag
     1321 ggaactgctg tgttcggctc agttgccggc gcggatcccg cccatctcgc tgaacacggc
     1381 ggtgacccca ccgttttcgc gctgctcgtc ggcgatcaca tgggagaccg cgttgatcag
     1441 cgccaggtgg gtgaaggcct gggggaagtt gccgaggtgg cggccggagt ccgcgtccag
     1501 ctcctcggcg aacaggtcga gccagccggc agcctcaaga agccgctcgc agagcgttcg
     1561 cgcccgttgg cactcgccga tctccgagag cgccgagacc agccagaacg agcagatcag
     1621 gaaggtgccc tccttgccgt gcaacccgtc gtcggtctcc tggaccttgt agcgcaggac
     1681 caggccgtgt tcggtcagtt cctcggcgat cgcgtcgacg gtcgcgtgca ggcgcgggtc
     1741 gtcgggcggg aggaaccgca gcagcgggat cagcagggtc gaggcgtcga gcgcgtcggt
     1801 gtcgtagtgc tggcggaaga cgttgtcgcg gacgccgcgc tcgaggatct cggccttgaa
     1861 ctcgtcggcc ttcgcccgcc actcggcggc gaggtcgtcg cggccgacgc tggcggcgag
     1921 ccgcgctccg cggtcgaccg cgacccacag catcagcttc gaggagacgt agtgctgcgg
     1981 ctccccgcgc gactcccaga tcccctggtc ggggtgcggc caggcgtcga tcgccgcctg
     2041 cacctggtag ccgatcagct cacgatcggc tttcgtgccg tgccctttca gcgctttctc
     2101 gtgcaggtag atcgagtcga gcagggcgcc ccagacgtcg ttctggcgct ggtcgaaggc
     2161 gccgttgccg atccgcaccg gctgcgctcc gccgtagccg ccgaggtggt cgagggtgct
     2221 ctcggtcag
//
LOCUS       DASRIG010000544         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1024957, whole genome shotgun sequence.
ACCESSION   DASRIG010000544 DASRIG010000000
VERSION     DASRIG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024957"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFB52_14575"
     CDS             <1..573
                     /locus_tag="VFB52_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07611.1"
                     /translation="GGGSRLIPDRTCVERDVLVGPGKTTSVWALYEKWSSENALETAA
                     GETLAAFPTAFGVFNPSRYGAGGATTARTLPLCWETAPDGDRADGVDCTAATQGGALA
                     EPFAWEDPKSPFDGTRRDFYIWGTAVSNAGGEEKVWTDPYGGNASPLPFPGAICQLVS
                     SLDNAAQSDARMQVFGRNRSFDAEGVHAPN"
     gene            800..2305
                     /locus_tag="VFB52_14580"
     CDS             800..2305
                     /locus_tag="VFB52_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZO07612.1"
                     /translation="MRKKLGIAALLTALASLMAPAMASAATVAELGETLEGTASELNM
                     TWVGVACVLVFLMQAGFMFLEIGFSRQKNAGTGVAKILVNFAIVTIAWWAVGYGIAEG
                     GNGEEGLPGNKIFGGEGFFFHFGQEVAGEVMNGESAMLMLFGLAFCAVSLAIVWGTTL
                     ERIKFSAYVIYAAVFGAVIYPLIAHAVWGGGFLGDIGGKPVMDFAGSSVVHLTGATGA
                     LAALLLLGPRIGKYDANGNPRAIPGHSMPLVGLGVIILWIGWFGFNGGSTFGTESNLF
                     GEVMLNTQLAAAAGVIGASLAIWLKSKTLDVGMAGNGAIAGLVGITAPSGFVEYWAAP
                     IIGIIAGFIVVFSVLAIDKLLDDPVGALSAHGLAGIWGTLAVGIFASPRLIVEGAGPG
                     IWYGIFGDESFSTALGQLGVQGLAVVFTFVVVLVISLAVFAGIKATIGLRVPAEEEDA
                     GLDISSHGMYGYPEVFIPQPEYSTGLPSTGSLPSSPAPAAAATSMTASETN"
     gene            2319..2687
                     /locus_tag="VFB52_14585"
     CDS             2319..2687
                     /locus_tag="VFB52_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZO07613.1"
                     /translation="MKKIEAFIRHEAFEPIRAELLDKGFPSLSIIEAKGSGRQKGIVE
                     RYRGSTLTVNVRPKIKLEVVIEDKDKGIVVETILKHARTGEVGDGKIFVLPVEEAIRI
                     RTGEDGQEVLQLHEESEIPS"
BASE COUNT          471 a          906 c          924 g          484 t
ORIGIN      
        1 ggcggcggtt cgcgcctgat ccccgaccgc acctgcgtcg aacgcgacgt gctggtgggg
       61 ccgggcaaaa ccacctcggt ctgggcgctg tatgagaaat ggagctcgga gaacgcgttg
      121 gaaacggcgg cgggggagac cctcgccgcg ttcccgaccg cgttcggagt cttcaacccg
      181 agccgctacg gagccggcgg cgcaaccacg gcgcggaccc tgccgctctg ctgggagacc
      241 gcccccgacg gcgaccgcgc cgacggcgtc gactgcaccg ccgccaccca gggtggcgcc
      301 ctggccgaac cgttcgcctg ggaggacccg aaatccccct tcgacggcac ccgccgcgac
      361 ttctatatat gggggaccgc ggtcagcaac gccggcggtg aggaaaaggt ctggaccgac
      421 ccctacggcg gcaacgcctc cccgcttccc ttcccaggcg cgatctgcca gctcgtctcc
      481 agcctcgaca acgccgccca gagcgacgcc cgcatgcagg tcttcggccg caaccgctcc
      541 ttcgacgccg aaggcgtcca cgccccgaat taggcgtcac gagtctgctt caaggggcgc
      601 tatgcgcacc ctggagcaga ctcgtgagcc cgctcccaga gccaaatcgt gcctgggatt
      661 ccaatcggga caaccgagtt cggcggattt cctccagccg tctaaataca tcgaggcggg
      721 tcgaatttac ggtgcggatc gaatcgctaa acccgcgacc tccggtgcgt gcgcgccggt
      781 gggctaggag gaattttcga tgagaaagaa gttggggatc gccgccttgt tgacggcgct
      841 cgcctcgctg atggcgccag ccatggcgtc ggcggcgacc gtcgcagaat tgggcgaaac
      901 gcttgaaggc acggcgagcg aactgaacat gacctgggtg ggcgtcgcct gcgtcctggt
      961 cttcctcatg caggccggct tcatgttcct ggagatcggc ttctcgaggc agaagaacgc
     1021 cggcaccggc gtggccaaga tcctcgtcaa cttcgcgatc gtgacgatcg cctggtgggc
     1081 ggtcggctac ggaattgccg aaggcggcaa cggtgaagaa ggcctccccg gaaacaagat
     1141 cttcggaggc gagggattct tcttccactt cggccaggaa gtagccggcg aagtgatgaa
     1201 cggggaatcc gcgatgctga tgctgttcgg gctcgccttc tgtgcggtct cgctggcgat
     1261 cgtctggggc accaccctgg agcggatcaa gttcagcgcc tacgtgatct acgccgcggt
     1321 gttcggcgcg gtgatctacc cgctgatcgc ccacgcggtc tggggcggcg gcttcctcgg
     1381 cgacatcggc ggcaagccgg tgatggactt cgccggctcc tcggtcgtcc acctcaccgg
     1441 tgcgaccggc gctttggcgg ccttgctgtt actcggcccg aggatcggca agtacgacgc
     1501 caacggcaac ccacgggcca tccccggtca ctcgatgccg ctggtcggcc tcggcgtgat
     1561 catcctctgg atcggctggt tcgggttcaa cggcggttcc accttcggca ccgaaagcaa
     1621 cctgttcggc gaggtcatgc tgaacaccca gctcgcggcc gccgccggtg tgatcggtgc
     1681 ttcgctcgcg atctggctga agagcaaaac gctcgacgtc ggcatggccg gcaacggtgc
     1741 gatcgccggt ctggtcggca tcaccgcacc atcgggcttc gtcgagtact gggcggcgcc
     1801 gatcatcggc atcatcgccg ggttcatcgt cgtgttctcg gtgctggcga tcgacaagct
     1861 cctcgacgac ccggtcggcg cgctctccgc gcacggcttg gccggtatct ggggcaccct
     1921 cgcggtcggc atcttcgcct cgccgcgtct gatcgtcgaa ggcgccggtc cgggcatctg
     1981 gtacgggatt ttcggagacg aaagtttcag taccgcgctt ggtcagctgg gcgtccaagg
     2041 actggcggtg gtgttcacgt tcgtggtcgt cctcgtgatc tcgttggccg tgttcgccgg
     2101 aatcaaagcg acgattggtc tacgggttcc agccgaggag gaggacgcgg gtctcgacat
     2161 cagctcgcac gggatgtatg gctatcccga ggtgttcatc ccacagccgg agtattcgac
     2221 cggcctgccg agcaccggtt cgctgcccag tagtccggca ccggcagccg cagccacgtc
     2281 catgaccgcg tccgagacca actaggagag tcgaacgcat gaaaaagata gaggcgttca
     2341 tccgccacga ggcgttcgag ccgatccggg ccgagctgct cgacaaggga ttcccgtccc
     2401 tgtcgatcat cgaggccaag gggtccggtc gccagaaagg catcgtcgag cggtaccgcg
     2461 gctcgacgct gaccgtcaac gtccggccga agatcaagct ggaggttgtg atcgaggaca
     2521 aagacaaagg catagtcgtc gaaacgatcc tcaaacacgc ccgcacgggc gaggtcggcg
     2581 acggcaagat cttcgtgctg ccggttgagg aggcgattcg catccgcacc ggcgaggatg
     2641 gccaagaggt cctccagctg cacgaggagt cggagattcc ctcctagggc agatccggga
     2701 cagacagcgg ttcggagcgg ggcggcccct gggtcgcccc gctcgtcgtt tatccacccc
     2761 gggggctagc ctcgtccgcg tggct
//
LOCUS       DASRIG010000545         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1814190, whole genome shotgun sequence.
ACCESSION   DASRIG010000545 DASRIG010000000
VERSION     DASRIG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814190"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFB52_14590"
     CDS             complement(<1..600)
                     /locus_tag="VFB52_14590"
                     /inference="COORDINATES: protein motif:HMM:NF037039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07614.1"
                     /translation="MTDALLSATPASPPLDLEPEVLALGRELGQQRFSLRTAKRGAHD
                     RAMALVSSDEQLRAALFRLVDVAPACAGPPELAAHLAAYLDDVEDEALPVALGHRAAD
                     SRALSRASGRFASAMVRQMAGRFIVGESIPDAEPVLSRLWRQGTAVTVDLLGEATVTH
                     AEGQVYADRCDEALHLLALASARWPDCPLLEGDAIGPVPR"
     gene            complement(657..1814)
                     /locus_tag="VFB52_14595"
     CDS             complement(657..1814)
                     /locus_tag="VFB52_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07615.1"
                     /translation="MGGRLDQQPDPRSIQAVVDALSIGLKRPILFDDAALVPVAYSRQ
                     WDVDEVRSSSILSRGPAPAVREALLAQRIDAARDVIHTDADPDLGMASRVCLPVRDGS
                     EVLGYLWLLDPDDDLTEEDLERLRGAARELAAILLGASRREVPDEADLFDLLRSPDPE
                     RREGAAAVARERHLLSDGGVILCLLAAHGPGADPVGAARQAVRRLSVGHAIAATAAEG
                     AALLASLGDPVLRTLPHAELATWVRAVAAADVAVGQSGEVRLSGLDEGARQAALALRV
                     ARRRGAEEAIAAFPTLGADRLVAQLPETAIRDIPPGLASLLREEPVLVATLAAFLDAG
                     GDVKATAAALSLHRSGLYYRLRRIQELTGLDLDRGDDRLLAHLAIRAEQMF"
BASE COUNT          349 a          779 c          862 g          301 t
ORIGIN      
        1 gcgcggcacc gggccgatcg cgtcgccttc gagcagcgga cagtcaggcc agcgggcgct
       61 tgccaaggcg agcagatgca gcgcctcgtc gcagcggtcc gcgtagacct gaccctcagc
      121 gtgggtcaca gtggcctcgc caagaagatc gacagtcacg gccgtgccct ggcgccagag
      181 gcggctcagg accggctcgg cgtcgggaat cgattcgccg acgatgaagc ggccagccat
      241 ctgccggacc atcgccgagg cgaagcggcc gctggcgcgg gagagggcac gcgagtcggc
      301 ggcgcggtga ccgagcgcga ccggcagcgc ctcgtcctcg acgtcgtcga ggtaggcggc
      361 gagatgcgcg gccagctcgg gaggcccggc gcaggccggg gcgacgtcga cgaggcggaa
      421 gagcgccgcc cgcaactgct cgtcgctgga gaccagcgcc atcgcccggt cgtgagcgcc
      481 ccgcttggcg gtccgcagcg agaaacgctg ctgtccgagt tcacgaccca gggccaggac
      541 ctcgggctcg aggtccagcg gcgggctggc gggagtcgcg gagagcagcg cgtcggtcac
      601 atggacaagc ttggaccccg ccccgcgcgg tcgccacgga cgaacgtagc gagccctcag
      661 aacatttgtt cagcgcgaat cgccaggtgg gcgagcagcc ggtcgtcgcc gcgatcgagg
      721 tcgagcccgg tcagctcctg gatgcggcgc aggcggtaat agaggccgct gcggtgcagg
      781 gagagggcgg cggcggtcgc cttgacgtcg ccaccggcgt cgaggaaggc ggcgagggtc
      841 gcgaccagga ccggctcctc gcgcagcagg ctggccagcc ccggcgggat gtcgcggatc
      901 gccgtctccg ggagctgggc gacgagccga tcggccccga gggtcgggaa ggcggcgatc
      961 gcctcctcgg cgccgcgcct gcgcgcgacc cgcagcgcca gcgccgcctg gcgcgcaccc
     1021 tcgtcgagac cgctcaggcg gacctcaccg gactggccaa cggcgacgtc ggcggcagcg
     1081 accgcccgca cccatgtcgc cagctccgcg tgcggcagcg tccgcaggac tgggtcaccg
     1141 agcgaggcga gcagggccgc gccctcggcg gcggtggcgg cgatcgcgtg gccgacagag
     1201 agccggcgga ccgcctggcg ggccgcgccg accgggtcgg cgccgggtcc gtgggcggcg
     1261 agcaggcaaa ggatcactcc gccgtcgctc agcaggtggc gctcgcgcgc aacggcggcg
     1321 gccccctcgc ggcgctcggg gtcgggagag cgcagcaagt cgaacaggtc ggcttcgtcg
     1381 gggacctcgc ggcggctggc accgagcagg atcgcagcaa gctcgcgcgc cgccccgcgc
     1441 agccgctcga ggtcttcctc ggtcaaatcg tcgtcggggt ccagtagcca gaggtatccg
     1501 agcacctcgc tgccgtcgcg gaccggcagg cagacccggc tggccatacc caggtcgggg
     1561 tcggcgtcgg tgtggatcac gtcgcgggcg gcgtcgatcc gctgggccag cagcgcctcg
     1621 cgaaccgccg gtgccgggcc gcggctgagg atgctcgagc tgcgcacctc gtcgacgtcc
     1681 cactggcggc tgtaggcgac gggcacaagg gcggcgtcat cgaagaggat cggccgcttg
     1741 aggccgatgc tgagcgcgtc gacgacggcc tggatcgacc gcgggtcagg ctgttggtcg
     1801 aggcggccac ccacgggagc actattaccg cagccgccgg gcggagccca ccatcgcctg
     1861 gcaggagaat ggccggtcct ggaggccgtg tcaggcgtga aggcccgact cgtcggccgc
     1921 gcgcaaattg gtacccgttt ggtacccggt gccgttgcat cattcgggtg gccggccacg
     1981 aaaaaggccc cggaaccggg gcctttcctt atagcggggg caggattcga acctgcgacc
     2041 ttcgggttat gaggtcgcca agcctgcttg actgggctga ttttcggctt gttcaagcgg
     2101 tctgagctgg ttcgagttgg gcctagttgc gctcaactta gtacccactt agtacccgcc
     2161 cccctcgcag acaggagcta catcgaggtt cggaatctca aacctctgcg gggcgagggc
     2221 tcagccctca tcgaaggccg agatgttctt cgccatggcc gagaccccca atgcgagaag
     2281 ctcgtcgatc a
//
LOCUS       DASRIG010000546         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1563051, whole genome shotgun sequence.
ACCESSION   DASRIG010000546 DASRIG010000000
VERSION     DASRIG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563051"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFB52_14600"
     CDS             <1..691
                     /locus_tag="VFB52_14600"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZO07616.1"
                     /translation="IANSIVSAGIGPAGSGNCTADPTDSPTSKGFNLESANDCRFTAP
                     GDQANTDPQLGPLGPNGGPTPTMLPSFTSPAVDRGVALGLASDQRGVVRPIDFPSIPN
                     AAGSAGSDVGAVELQPSNAFGFGKLTKNKKKGTARLTVNLPTPNVGVIALRGNGLKPQ
                     TKAVTGTTSKVTFVVALKSKKLKKALRKKGKRKVGIKVTYTPVGNAALTKVRKAKLVR
                     KQQKPKRQVKR"
     gene            complement(701..994)
                     /locus_tag="VFB52_14605"
     CDS             complement(701..994)
                     /locus_tag="VFB52_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07617.1"
                     /translation="MLSVEGESNESLVLDGEWFEKQRMGEPKARIAASTFQKAEWKEF
                     EKRKRLFGGEKVKMVQVVITFEKPPFVGLITEAEKRPKLEEIVAGLEAAAASA"
     gene            1050..2807
                     /locus_tag="VFB52_14610"
     CDS             1050..2807
                     /locus_tag="VFB52_14610"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZO07618.1"
                     /translation="MPKRTLLFAALAAALLLAPASAGAATVANTNDAGPGSLRQAIID
                     AAPGETITIPTGTYTLTSGELAIAKSLTLTGEGPAATVIRSGGAFRVFHLTGVANEVT
                     FTDLAIRDGKAALAALSRGGGIFSEQASLTLRRVLVAGNEANPTTAATVALGGGISVV
                     GMAATRVGIFDSTFSGNIANGAGGDEQNGGLSEGGGADINGALTTIANSTFAGNLTDS
                     SGGDKPTAPPNTQNGTIGAGGAVNLRTPLAGSTISSSTFSGNVVDSSGGPGSVSSIGE
                     GGGLQIRAEGLSNITLNQLTVTGNSIRQSTPSIASGGGAKLRTSGPGTVAILSSTFAG
                     NAVPTDGPNQAGGNIAWEKGSTIANSVISGGTGGIGGNGANCSATTPVTSLGFNLESL
                     DQCELDGPGDRVNTDPQLGPLQANGGPTATMLPATTSPLVDQGSAKGLTADQRGLPRT
                     VDFVSVPNPAVAGADGTDIGAVELQPTATVALGKLTRNRKRGTASLTVSLPALNLGTA
                     TLSGKGLKTQTVALTGPGSTVTIPVVTVGKVKKALRKKGKRKVGIAVTYAATPALVAG
                     ATRKATLVRKKPKPKKTRK"
BASE COUNT          546 a         1093 c          910 g          359 t
ORIGIN      
        1 gatcgccaac tcgatcgtca gcgccggcat cggcccggcc gggtccggca actgcaccgc
       61 cgaccccacc gacagcccga cctcgaaagg gttcaacctc gagagcgcca acgactgcag
      121 attcacggcg cccggcgacc aggccaacac cgacccccag ctcgggccgc tcgggcccaa
      181 cggcggtccg accccgacga tgctgccgag cttcaccagc cccgccgtcg accgcggcgt
      241 cgcgctcggc ctcgccagcg atcagcgcgg cgtcgtccgg ccgatcgact tcccctcgat
      301 tcccaacgcg gcgggcagcg ccggctccga cgtcggcgca gtcgagctgc agccctcgaa
      361 cgcgttcggc ttcgggaaac tgacgaagaa caagaagaaa gggacggcgc gcctgaccgt
      421 caacctgccg actccgaacg tcggtgtgat cgccctgcgc ggcaatgggc tgaagccgca
      481 gacgaaagcg gtgacgggca ccacctccaa agtcaccttc gtcgtcgccc tgaagagcaa
      541 gaagctgaag aaagcgctgc gcaagaaggg caagcgcaaa gtcgggatca aagtcaccta
      601 cacgccggtc ggcaacgcgg ccctgaccaa agtccgcaaa gccaagctgg tccgcaagca
      661 gcagaaaccg aaacggcagg tcaagcggta gcgggccggc ttaggcgcta gccgcggcgg
      721 cttcgaggcc ggcgacgatc tcttccagct tcgggcgctt ctccgcctcg gtgatcaggc
      781 cgacgaaggg cggcttctcg aaggtgatca cgacctggac catcttcacc ttctcgccgc
      841 cgaacagcct cttgcgcttc tcgaactcct tccactccgc tttctggaag gtcgaggcgg
      901 cgatccgggc cttcggctcg cccatccgct gcttttcgaa ccactcgccg tcgaggacga
      961 gcgactcgtt gctttctcct tcgacgctca gcactggacg ggaatctagt cgtttgaacc
     1021 tcgggcgtca gaatcgagta gaaggagcta tgcccaaaag aacccttctg ttcgccgccc
     1081 tcgccgcagc gctcctgctt gcccccgcga gcgccggcgc cgccaccgtc gccaacacca
     1141 acgatgccgg tcccggctcg ctgcgccagg cgatcatcga cgccgctccg ggcgaaacga
     1201 tcacgatccc gaccggcacc tacacgctga cctccggcga gctggcgatc gcgaaaagcc
     1261 tgacgctgac cggcgaggga ccggccgcca ccgtgatccg ctccggtggc gccttccgcg
     1321 tcttccacct gaccggcgtc gccaacgaag tcaccttcac cgacctcgcg atccgcgacg
     1381 gcaaagccgc gctggcggcg ctcagccgcg gcggcggcat cttcagcgag caagcgagcc
     1441 tcaccctacg gcgcgtgctc gtcgccggca acgaagcaaa cccgacgact gccgcgaccg
     1501 tcgccctggg cggcggcatc tcggtcgtcg gcatggccgc gacccgggtc gggatcttcg
     1561 acagcacctt cagcggcaac atcgccaacg gcgccggcgg cgacgaacag aacggcggcc
     1621 tctccgaagg cggcggcgcc gacatcaacg gcgccctgac gacgatcgcc aactcgacct
     1681 tcgccggcaa cctgaccgac agcagcggcg gcgacaaacc gaccgcgccg ccaaacaccc
     1741 agaacgggac gatcggcgcc gggggcgcgg tcaacctgcg caccccgctg gccggcagca
     1801 cgatctccag ctcgaccttt tccggcaacg tcgtcgactc ctccggcggc cccggctcgg
     1861 tcagcagcat cggcgaaggc ggcggcctgc agatccgcgc cgagggcctg tcgaacatca
     1921 cgctcaacca gctcacggtg accggcaact cgatccgcca aagcacgccg agcatcgcct
     1981 ccggcggtgg agcgaagctg cggacctcgg gaccgggcac cgttgcgatc ctcagctcga
     2041 ccttcgccgg caatgctgtg ccgaccgacg gaccgaacca ggccggcggc aacatcgcct
     2101 gggaaaaagg ctcgacgatc gccaactccg tgatcagcgg cggcacgggc ggcatcggcg
     2161 gcaatggcgc caactgcagc gcgacgacgc cggtcacctc gctcggcttc aacctcgaga
     2221 gcctcgatca gtgtgaactc gatggcccgg gtgatcgcgt caacaccgac ccgcaactcg
     2281 gcccgctgca ggccaacggc ggcccgaccg cgacgatgct gccggccacg accagcccgc
     2341 tggtcgacca gggcagcgcc aagggactca ccgccgacca gcgcggcctg ccgcggacgg
     2401 tcgacttcgt ctcggtgccg aacccggcgg tggccggagc agacggcacc gacatcggcg
     2461 cggtcgagct gcagccgacc gccacggtcg cgctcggcaa actgacccgc aaccgcaagc
     2521 gcggcaccgc ttcgctgacc gtcagcctgc cggcgctcaa cctcggcacc gccaccctct
     2581 ccggcaaggg cctcaaaacc cagaccgtgg ccctgaccgg ccccggcagc acggtgacga
     2641 tcccggtcgt cacggtcggc aaagtcaaga aggcgctgcg caagaagggc aagcgcaaag
     2701 tcgggatcgc ggtgacctac gcggcgaccc cggcgctcgt cgccggcgcc acccgcaagg
     2761 caaccctcgt acgcaagaag ccgaagccga agaaaaccag gaaatagcgc cgcggcgggc
     2821 cgagctgggc ccgcggctac catcgaagga tggaggcgaa agcagccgag ggcatcgcct
     2881 tcgagagcag cgccggccgc tggatgct
//
LOCUS       DASRIG010000547         7750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_487986, whole genome shotgun sequence.
ACCESSION   DASRIG010000547 DASRIG010000000
VERSION     DASRIG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487986"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1586
                     /locus_tag="VFB52_14615"
     CDS             117..1586
                     /locus_tag="VFB52_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZO07619.1"
                     /translation="MNLEVSRREPELEIHELDRRRRIRVLAICCLSLFIVGLDVTVVN
                     VALPSIGSDLDTGISGLQWTVDAYTVVLASLLMFSGSVGDRFGRRRVFMVGLTLFVVA
                     SGLCSIAPNIESLVAFRVLQAVGASMLNPVALSIVTNTFTDPRERAQAIGAWASVFGI
                     SMALGPVVGGALVSALDWRAIFWINIPVGLAAILLTLRFVPESRAPRPRRFDPLGQVL
                     VIVLLASLTSGIIEAPDRGWTSAATLATFALSAAALAGLLLHEPRRRDPLIDLRFFRS
                     VPFASAAATAVAAFAAFGGFLFLNTLYLQEVRDLSPVQAGLAILPMAAMTVLFAPISG
                     RIVGHRGPRLPLAIGGGFLLAGCLMLTDLQPDTPFAWLLAAYVVFGIGFGFVNAPITN
                     TAVSGMPRAQAGVAAAVTSTSRTVGQTLGVALVGALVASHIGDSADAELAAASHVAWW
                     TLTAAGAVVLGLAFLATTARAHASAQRTAAELNPEALLP"
     gene            1598..2053
                     /locus_tag="VFB52_14620"
     CDS             1598..2053
                     /locus_tag="VFB52_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZO07620.1"
                     /translation="MPRPDDKPTTSDAAAVWLAMQDLVLDNARRREVSEALGVSFARA
                     RAIRRIARRPMSMGELAAALAIDPPNATGVVDDLEGLGLVKRRPHPDDRRTKLVEATP
                     KGRELARRADEILATPPPALSDLSAKDLETLRRILGEVAGDDEGSRPPR"
     gene            2084..2710
                     /locus_tag="VFB52_14625"
     CDS             2084..2710
                     /locus_tag="VFB52_14625"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZO07621.1"
                     /translation="MRIEIVVFDGFDELDAIGPLEILRNLAVGKPEVNVALVTPEPAA
                     GVLASHGLRIEVDGVLSDGADLLLVPGGGWAQKGEHGVHAEIARGDLPRRIAAAHAGG
                     ATVAGVCTGVLLLGAAGLLEGRRATTLRRAHEQLAGYGARVLDERVVDDGDVITCGGV
                     TSGLDLAFWLVEREFGSSHARAIAAGMEYERRGGVYIGSGGARSNEPT"
     gene            complement(2757..3872)
                     /locus_tag="VFB52_14630"
     CDS             complement(2757..3872)
                     /locus_tag="VFB52_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07622.1"
                     /translation="MKESSPTRRGHPRWAFALIVLGAILTFAAIFSIWVNRQALNTDN
                     WVDTSNRLIQDEEVRDQLSDYLANQLVANADVAGELEAALPPRLKPLATAAAGGLGQL
                     APQVAERIFATPKFEQLWEAANRKAHEALLQVLNDEGEVVSTGNGEVKLELGPVLEQI
                     GEGSGVGSALVSKLPAEAGEITIVKSDELSLAQDIANLLRKLPVVLTLLALLSFGLAV
                     FLSPRRRETLRSVGFAFVIAGLLSLGLRGFAGTYVVGDLSKTASVEPAVESVWSIGTS
                     MLGEIATSAIVFGVLVALAAILAGPTKPATRLRAWIAPYAERSPAGLWGAAFVIFLAL
                     IIWAPVGAFHKPLGVLIFALLFAAGTELYRRQLLDEA"
     gene            complement(3902..4291)
                     /locus_tag="VFB52_14635"
     CDS             complement(3902..4291)
                     /locus_tag="VFB52_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZO07623.1"
                     /translation="MPLADISFGEALLVVLEFFLLFAWIWILISVIIDLFRDHALSGV
                     AKAVWVFFLVFIPMIGVLIYLIVRGGGMRDRALKEQADTKKHFDEYVREQAHTSPADE
                     LHKLNELKEKGALSPEEFEQAKAKLLS"
     gene            complement(4370..4753)
                     /locus_tag="VFB52_14640"
     CDS             complement(4370..4753)
                     /locus_tag="VFB52_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07624.1"
                     /translation="MSKRTLMIGIATGLVALVPLSATATANDADVKRAGTCSGNTSAK
                     IKLSPDDGRIETEFEVDQNRNGVTWRVVLRRNGAVAVRTQGTTRGPSGSFEVERRLTD
                     GQGTDTVTARAVSPSGEVCTAQASI"
     gene            complement(4798..5004)
                     /locus_tag="VFB52_14645"
     CDS             complement(4798..5004)
                     /locus_tag="VFB52_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZO07625.1"
                     /translation="MSYKATVDPDECSAHGDCVEIAPSVFRLDDVAVVIGADSPELMT
                     EAAEACPAVAIAIVDESTGESVFP"
     gene            complement(5001..6188)
                     /locus_tag="VFB52_14650"
     CDS             complement(5001..6188)
                     /locus_tag="VFB52_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZO07626.1"
                     /translation="MSAGVLIVGGGLAAQRVAETLRRRGYDGAVRIACAETEAPYDRP
                     PLSKELLAGSAPEDSVAYRPVDWYAEAEVELLLGATATGLDARHRRVRLDGGEELGYE
                     SLVIATGGAPRRLPFLTGANVHELRTLADLRRLRAQLVPGARLAIVGAGFIGQEVAAT
                     ARGLGLEVTVIEALPLPLQPIVGPRLGRWFADLHTEEGVRVLTGAALDSVRGADRVEE
                     LLLADGRRIECDLVVVGIGTVPATAWLRGSGLGESGVVVDAGGRTALPGVFAAGDACL
                     ALEPRRGVHARTEHWDAAAWQGASVAKAILGEQPGRPPLPSFWSDQYGLRVQCVGHPA
                     LAGEVAIEGDPGDRDFEALFLRDGVPVAGLAVGRPRAIPALRKQIEGCGLAAPAQPEM
                     EAA"
     gene            complement(6185..6841)
                     /locus_tag="VFB52_14655"
     CDS             complement(6185..6841)
                     /locus_tag="VFB52_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HZO07627.1"
                     /translation="MTEAARTLESHGWWLASRASGLLALVLVTVSVAIGLAMAGKVLR
                     VPGLSRKLMAVHEQTALAGLIAIAVHAITLIGDPFLNPGVAGVAVPFVLDFETVFTGL
                     GVIAAYLAMLLGLSFYFRRRIGAKLWRKAHRATVLVWVLGLVHALGAGSDTSELWFRW
                     WVMATTPLIGGLFVYRVLEGRAKAAKKAKPAEAEKPAPVKPGTKARPVKPGHAVEAPS
                     "
     gene            complement(6838..>7750)
                     /locus_tag="VFB52_14660"
     CDS             complement(6838..>7750)
                     /locus_tag="VFB52_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZO07628.1"
                     /translation="AAPAAEAAERARRFVVAFETALSRFNPESELCALNDDPRERVPA
                     SQLMRRAVSAARAVAERSDGLVDPTLAGEIERAGYIESRVGVAPASLAQALAVAPPRR
                     SAAPAPQRRWRELAIDEEAGVVVRPPGLRIDSGGFGKGLCADLMAADLAPYSRFVVDC
                     GGDIRIGGRRASEQPFAVEVEHPLNGDRSFVVRLGSGGIATSGLNVRIWRDAAGRFAH
                     HLLDPASGEPAWTGLIGATALGTSALEAETLSKAALLSGPERGATLLREHGGILVHDD
                     GRVERVGPLAAPPRIRLPEIALQGAAA"
BASE COUNT         1047 a         2881 c         2712 g         1110 t
ORIGIN      
        1 tcacctgcac ggtctccgct cgccgccgac cggtctgctc gcagatcgtc ctcgccgcga
       61 tcacctcctt ggtcacccga tcctgaggag atcgtcctgc taagttcata atagttatga
      121 atctagaagt atctcgccgg gaaccagagc tggagatcca cgagctggac cggcgccggc
      181 ggatcagggt cctggcgatc tgctgcctca gtctcttcat cgtcgggctc gacgtcaccg
      241 tcgtcaacgt cgccctgccc tcgatcggca gcgacctcga caccggcatc tccgggctgc
      301 agtggaccgt cgacgcctac acggtggtgc tggcgagcct gctgatgttc tccggctcgg
      361 tcggcgaccg cttcggccgc cgccgcgtct tcatggtcgg gctgacgctc ttcgtcgtcg
      421 cctcggggct ctgcagcatc gccccgaaca tcgagtcgct cgtcgccttc cgcgtcctgc
      481 aggcggtcgg cgcctcgatg ctgaacccgg tcgcgctctc gatcgtcacc aacaccttca
      541 ccgatccgcg cgagcgcgcg caggcgatcg gcgcctgggc ctcggtcttc gggatctcga
      601 tggcgctggg gccggtggtc ggcggcgccc tcgtctccgc cctcgactgg cgggcgatct
      661 tctggatcaa catcccggtc gggctggccg cgatcctgct gaccctgcgc ttcgtccccg
      721 aatcccgggc gccgcggccg cgccgcttcg acccgctcgg ccaggtgctg gtgatcgtcc
      781 tgctcgcgag cctcacctcg gggatcatcg aggcgcccga ccggggctgg acctcggcgg
      841 cgaccctggc gaccttcgcc ctctccgcgg cggcgctcgc cggcctgctc ctgcacgagc
      901 cgcggcgccg ggacccgctg atcgacctgc gcttcttccg ctcggtcccc ttcgcctcgg
      961 cggccgcgac cgcggtcgcg gccttcgccg ccttcggcgg gttcctcttc ctcaacaccc
     1021 tctacctgca ggaggtccgc gacctctcgc cggtccaggc cgggctcgcg atcctgccga
     1081 tggcggcgat gaccgtgctc ttcgcgccga tctcggggcg gatcgtcggc caccgcggcc
     1141 cgcgtctgcc gctggcgatc ggcggcggct tcctgctcgc cggctgcctg atgctgaccg
     1201 acctgcagcc cgacaccccg tttgcctggc tgctcgccgc ctacgtcgtc ttcgggatcg
     1261 gcttcggctt cgtcaacgcg ccgatcacca acaccgcggt ctcggggatg ccgcgcgccc
     1321 aggccggcgt cgccgccgcg gtcaccagca cctcgcgcac ggtcggccag acgctcggcg
     1381 tcgccctggt cggcgcgctc gtcgcctccc acatcggcga ctccgccgac gccgagctcg
     1441 ccgccgccag ccacgtggcc tggtggaccc tgaccgccgc cggcgccgtc gtcctcggac
     1501 tcgcgttcct cgccaccacc gcccgcgccc acgcctccgc ccagcgcacc gccgccgagc
     1561 tcaaccccga ggcgctgctc ccctagcttc gaccgcgatg ccacgacccg acgacaagcc
     1621 caccaccagc gatgccgccg cggtctggct ggcgatgcag gacctggtcc tcgacaacgc
     1681 ccgccgccgc gaggtctccg aggcgctcgg cgtcagcttc gcccgcgccc gcgcgatccg
     1741 ccgcatcgcc cgccggccga tgtcgatggg cgagctcgcc gccgcactcg cgatcgaccc
     1801 gccgaacgcg accggagtcg tcgacgacct cgagggcctc ggcctggtca agcgccgccc
     1861 ccatcccgac gaccgccgca ccaagctggt cgaagcgacc ccgaagggcc gtgagctcgc
     1921 ccgccgcgcc gacgagatcc tcgccacccc cccacccgcg ctcagcgatc tgagcgccaa
     1981 ggacctcgag acgctgcgcc ggatcctggg cgaggttgcg ggggacgacg agggctctcg
     2041 cccgccgcga taacttcctc tgagcaacga aagggaggcg gggatgagga ttgagatcgt
     2101 ggtcttcgac gggttcgacg agctcgacgc gattgggcca ctggagattc tgaggaacct
     2161 cgccgtcggc aagcccgagg tgaacgtcgc gctggtcacg cccgagcccg ccgccggcgt
     2221 cctcgcttcg cacggcctgc gcatagaggt cgacggcgtg ctcagcgacg gagcggacct
     2281 gttgctcgtc cccggcggcg gctgggcgca aaagggtgag cacggcgtcc acgccgagat
     2341 cgcccgcggc gacctgccgc ggcggatcgc agccgcgcac gccggcggcg ccacggtagc
     2401 cggcgtctgc accggggtgc tgctgctcgg ggccgcaggc ctgctcgagg ggaggcgggc
     2461 gacgaccctc cgaagagccc acgagcagct cgccggttac ggcgcccggg tgctcgatga
     2521 gcgggtggtc gacgacggcg acgtgatcac ctgcggcggc gtcacctctg gcctcgacct
     2581 cgccttctgg ctggtcgagc gcgagttcgg cagcagccac gcccgggcga tcgccgccgg
     2641 catggagtac gaacgacgcg gcggcgtcta catcggctcc ggcggcgcgc gctcgaacga
     2701 gccgacgtag gctgccggca gggagcgcgg cccgctcgcc tgagcgagcc gggccgctac
     2761 gcctcgtcga gcagctggcg ccggtagagc tcggtcccgg ccgcgaacag cagggcgaag
     2821 atcagcacgc cgagcggttt gtggaaggca ccgaccggcg cccagatgat cagcgcgagg
     2881 aagatcacga acgccgcgcc ccagaggccg gcggggctgc gctcggcgta gggggcgatc
     2941 caggcgcgga gcctggtcgc cggtttggtc gggccggcga ggatcgcggc cagcgcgacc
     3001 aacactccga agacgatcgc cgaggtcgcg atctcaccca gcatcgaggt gccgatgctc
     3061 cagacgctct cgaccgccgg ttcgaccgac gccgtcttgc tgaggtcgcc gaccacgtag
     3121 gtgccggcga agccgcgcag accgagcgag agcagccccg cgatgacgaa ggcgaagccg
     3181 accgagcgca gggtctcgcg ccgacgcggc gagaggaaga cggcgagccc gaagctgagc
     3241 agcgccagga gcgtcagcac caccggcagt ttgcgcagca ggttggcgat gtcctgcgcc
     3301 aacgagagtt cgtcggattt gacgatcgtg atttcgccgg cttcggccgg cagtttggaa
     3361 accagcgcgc tgcccacccc cgagccttcg ccgatctgtt cgagcaccgg gccgagctcc
     3421 agcttcactt cgccgttccc ggtcgagacc acctcgcctt cgtcgttgag gacctgcagc
     3481 agcgcttcgt gggctttgcg gttggccgct tcccagagct gctcgaactt gggcgtggcg
     3541 aagatccgct cggccacctg gggggcgagc tgccccagac ccccggccgc cgcggtggca
     3601 agcggcttca ggcgcggcgg cagcgccgct tcgagctcac cggcgacgtc cgcgttggcg
     3661 accagctggt tcgcgaggta gtcggagagc tggtcgcgga cctcctcgtc ctggatcagg
     3721 cggttgctcg tgtcgaccca gttgtcggtg ttgagcgcct ggcggttgac ccagatcgag
     3781 aagatcgcgg cgaaggtgag gatcgcgccg aggacgatca gcgcgaatgc ccagcgcgga
     3841 tgcccccggc gagttggcga actctccttc accgtcatag cctgatcctc gtcgccgagg
     3901 ctcaggaaag caactttgcc ttggcctgct cgaactcctc aggcgagagc gcgcccttct
     3961 ctttcagctc gttcagcttg tgcagttcgt cggccggcga ggtgtgggcc tgctcgcgca
     4021 cgtattcgtc gaagtgcttt ttggtgtccg cctgctcttt gagcgcccgg tcgcgcatcc
     4081 cgccgccgcg cacgatcagg tagatcaaca cgccgatcat cgggatgaag acgaggaaga
     4141 acacccaaac cgccttcgcg accccggaga gcgcgtggtc acggaacaga tcgatgatca
     4201 ccgagatcag gatccagatc caggcgaaca gcaggaagaa ctcgaggacg actagcagtg
     4261 cttcgccgaa gctgatgtct gcgagcggca tttatctcct tggggattgc ggtggctagc
     4321 gccagcctag tcctatctgg tgccggcgcg ccaccacaga ccccgcgggt cagatactcg
     4381 cctgcgccgt gcagacctct ccggagggac ttacggcgcg cgcggtgacc gtgtcggttc
     4441 cctggccgtc ggtcagtcgt cgctccacct cgaacgagcc gctcgggccg cgggtcgtgc
     4501 cctgggtgcg gacggcaacc gctccgttgc ggcgcagcac gacccgccag gtgaccccgt
     4561 tgcggttctg gtcgacctcg aactcggtct cgatccggcc gtcatccggg ctcagcttga
     4621 tcttcgccga ggtgttgccg ctgcaggttc cggcgcgctt cacgtcggcg tcgttcgccg
     4681 tcgccgtcgc gctcagcggc accagggcta ccagtccggt ggcgatgccg atcatcaaag
     4741 ttcgcttgct catcttcttg ctccttgcat tcgggaatca atcattgggg tgacggacta
     4801 gggaaagacg ctctcgccgg tcgactcgtc gacgatcgcg atcgcgaccg ccgggcaggc
     4861 ttccgccgcc tcggtcatca gctccggcga gtcggcgccg atcacgacgg cgacgtcgtc
     4921 gagccggaac acgctcggcg cgatctcgac gcagtcgccg tgggcgctgc actcgtccgg
     4981 gtcgacggtg gccttgtagc tcatgctgcc tccatctcgg gttgggcggg agcggcgagg
     5041 ccgcacccct cgatctgctt gcgcagggct gggatcgcgc gcggccggcc gaccgcgagg
     5101 ccggcgaccg gtacgccgtc gcgaaggaag agcgcttcga agtcacggtc gccggggtcg
     5161 ccctcgatcg cgacctcgcc ggccagcgcc gggtgaccga cgcactggac gcggagcccg
     5221 tactggtcgc tccagaagct cggcagcggc ggtcgccccg gctgctcgcc gaggatcgcc
     5281 ttggcgacgc tcgcgccctg ccaggcggcg gcgtcccagt gctcggtgcg ggcgtgcacg
     5341 ccgcggcgcg gttccagcgc caggcaggcg tcgccggcgg cgaagacgcc gggcagcgcg
     5401 gtgcgcccgc cggcgtcgac gacgaccccg gactccccca gcccgctgcc ccgcagccac
     5461 gcggtcgccg gcacggtgcc gatcccgacc acgaccaggt cacactcgat ccgccgcccg
     5521 tcagcgagca gcagctcctc gacccggtcg gcgccgcgga ccgagtcgag cgcagcgccg
     5581 gtcaggaccc gcaccccctc ctcggtgtgc aggtcggcga accagcggcc gaggcgcggg
     5641 ccgacgatcg gctgcagcgg cagcggcagc gcctcgatca cggtcacctc gaggccgagc
     5701 ccgcgcgccg tcgccgccac ctcctggccg atgaagccgg cgccgacgat cgccagccgg
     5761 gcgccgggga cgagctgcgc ccgcaggcgc cgcaggtcgg cgagggtgcg cagctcgtgg
     5821 acgttggcgc cggtgaggaa gggcagccgc cgcggcgcgc cgccggtggc gatcaccagc
     5881 gactcgtagc ccagctcctc gccgccgtcg agccggaccc ggcggtggcg cgcgtcgagg
     5941 ccggtggcgg tggcgccgag cagcagctcg acctccgcct ccgcgtacca atcgacgggc
     6001 cggtaggcga ccgagtcctc gggcgcgctg ccggcgagca gctccttgga gagcggcggc
     6061 cggtcgtagg gcgcctcggt ctcggcgcag gcgatccgga ccgcgccgtc gtagccgcgc
     6121 cggcgcagcg tctccgcgac ccgctgggcg gccaggccgc cgccgacgat cagcaccccg
     6181 gcgctcacga gggcgcctcc acagcgtggc ccggcttgac cggcctcgcc ttcgtccctg
     6241 gtttgaccgg cgcgggcttc tccgcctcgg ccggcttcgc cttcttggcc gccttcgccc
     6301 ggccttcgag cacgcggtag acgaagaggc cgccgatcag cggcgtcgtc gccatcaccc
     6361 accagcggaa ccagagctcg ctggtgtcgc tgccggcgcc gagcgcgtgg accaggccga
     6421 gcacccagac gagcaccgtc gcgcggtgcg ccttgcgcca gagcttggcg ccgatccggc
     6481 ggcggaagta gaagctgagg ccgagcagca tcgccaggta ggcggcgatc acgccgaggc
     6541 cggtgaaaac ggtttcgaag tcgagcacga aggggaccgc gacgccggcg acgccggggt
     6601 tgaggaaggg atcgccgatc agcgtgatcg cgtgcacggc aatcgcgatc agcccggcta
     6661 gcgccgtctg ctcgtgcacc gccatcagct tgcgcgagag gccggggacg cgcagcacct
     6721 tgccggccat cgccagtccg atcgcgaccg agacggtgac caggaccagg gcgagcaggc
     6781 ccgaggcgcg gctcgccagc caccagccgt ggctctccag ggtgcgggcc gcctcggtca
     6841 tgccgccgct ccctgcaagg cgatctcggg caggcggatg cgcgggggcg cggccagcgg
     6901 cccaacccgc tcgacccgcc cgtcgtcgtg gacgaggatc ccgccgtgct cgcgcagcag
     6961 ggtggcgccg cgctcgggcc cgctcagcag cgccgccttc gagagcgtct ccgcctcgag
     7021 ggcgcttgtg ccgagcgcgg tcgccccgat cagcccggtc caggccggct cgccgctcgc
     7081 ggggtcgagc aggtggtggg caaagcggcc cgccgcgtcg cgccagatcc gcacgttgag
     7141 gcccgaggtg gcgatcccgc cggagccgag ccggacgaca aagctgcggt cgccgttcag
     7201 cgggtgctcg acctcaaccg cgaacggctg ctcgctcgcc cgccggccgc cgatccggat
     7261 gtcgccgccg cagtcgacga cgaagcgcga gtagggggcg aggtcggcgg ccatcaggtc
     7321 ggcacagagg cccttgccga agccgccgct gtcgatccgc aggccaggtg gccggacgac
     7381 gacgccggcc tcctcgtcga tcgcgagctc gcgccagcgc cgctgcgggg ccggagcggc
     7441 ggagcgccgg ggcggggcga ccgcgagcgc ctgcgccagc gaggcgggcg cgacgccgac
     7501 ccggctctct atatagccgg cccgctcgat ctcgcccgcc agggttgggt cgaccagccc
     7561 atcgctgcgc tccgccacgg cccgcgcggc gctgaccgca cggcgcatca gctgcgaagc
     7621 cgggacccgt tcgcgcgggt cgtcgttcag cgcgcagagc tcgctctccg ggttgaagcg
     7681 cgagagggcc gtctcgaagg cgacgacgaa gcggcgggcg cgctcggcgg cctccgccgc
     7741 gggcgcggcg
//
LOCUS       DASRIG010000548         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1814905, whole genome shotgun sequence.
ACCESSION   DASRIG010000548 DASRIG010000000
VERSION     DASRIG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814905"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="asnS"
                     /locus_tag="VFB52_14665"
     CDS             <1..1125
                     /gene="asnS"
                     /locus_tag="VFB52_14665"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZO07629.1"
                     /translation="AVGQEAAIRVRGSVREDKRAPSGVELTLKDLEAVSGSQDYPITP
                     KEHGTPFLLDHRHLWIRSQRQHAILRVRHTLVDACRRFLNDEGFINADAPIFTPNACE
                     GTTTLFETQYFDEKAYLTQSGQLYNEATAAAFGKVYCFGPTFRAEKSKTRRHLTEFWM
                     VEPEVAYARLDDIMTLAEEFISSVVGRVLENRREELKQLERETTALERVAPPFPRLQY
                     DEAARMLQEGHEQGKLEHRFEWGGDLGAPDESYLSAQFDRPVMIHRYPAAVKAFYMEP
                     DPARPELALCVDVLAPEGYGEIIGGGERASSLDLLLERIHEHGLPVEAFDWYLDLRKF
                     GSVPHAGFGMGIERAVAWICGLEHVRETIPFPRMLYRLKP"
     gene            1154..1864
                     /locus_tag="VFB52_14670"
     CDS             1154..1864
                     /locus_tag="VFB52_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZO07630.1"
                     /translation="MSGIPPKPGAPGHRPPPISQWQGEFEKKVAQEFERRTGLDYKET
                     VALIINAIDPFPGMQVLDVATGTGVIARQMVRLVGEKGRIVGVDGTDKMIEQARLAAQ
                     SAGLGRRIEWKVAKAEKLPFATESFDLITCVLAFHLLQAEQFLEQAWRVLKPGGRLLL
                     AHELAPRGGLSPLRLTARRNYYQFIARDPGEAGAHFYSADEMVAMLLAAGFRQYVVRG
                     LREPNKFELSFSLITAGK"
     gene            1867..2775
                     /gene="rocF"
                     /locus_tag="VFB52_14675"
     CDS             1867..2775
                     /gene="rocF"
                     /locus_tag="VFB52_14675"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZO07631.1"
                     /translation="MRKVTIIGVLMDLGADRRGVDMGPSALRVASLNERLAELGCEVT
                     DAGNISVHNPEMLPITDRRLKYLPEIAAASQQLADRVEAILEGGSTPIVLGGDHSIAI
                     GSVAGLAAFHRRRDARVGVIWFDAHGDMNTPETSPSGNIHGMPFAAILGRGAPELTHV
                     SGFAPKVSPEDCVLIGARSVDREEAAALRASGIRVVTMRELDERGMSAVMDEAMWLAS
                     RRTAGFHVTMDMDFVDPDFAPGVGTPVPGGPTYRESHLAMEKIADSGKMLSFELTEVN
                     PVLDVSNRTAELGVQLILSAFGKKIM"
BASE COUNT          467 a          957 c         1015 g          439 t
ORIGIN      
        1 gcggtcgggc aggaggcggc catccgggtg cggggatccg tgcgcgagga caagcgcgct
       61 ccgagcgggg tcgagctgac cctgaaggac ctcgaggccg tctcgggcag ccaggactac
      121 ccgatcacac cgaaggagca tggcacgccg ttcctcctcg accaccggca cctctggatc
      181 cggtcgcagc gccagcacgc catcctccga gtccgccaca ccctcgtcga cgcctgccgc
      241 cggttcctga acgacgaggg gttcatcaac gcggacgccc ccatcttcac gccgaacgcc
      301 tgcgagggga cgaccaccct cttcgagact cagtacttcg acgagaaggc ctacctgacg
      361 cagtcgggcc agctctacaa cgaggcgacg gccgcagcct tcggcaaggt ctactgcttc
      421 ggcccgacct tccgcgccga gaagtcgaag acccgccgcc acctgaccga gttctggatg
      481 gtcgagccgg aggtcgccta cgcgcggctc gacgacatca tgacgctggc tgaggagttc
      541 atcagctcgg tcgtcgggcg ggtgctcgag aaccggcgag aggagttaaa gcagctcgag
      601 cgcgagacga cggcgctcga gcgagtcgcg ccgcccttcc cgcgcctcca gtacgacgag
      661 gcggcgcgga tgctccagga gggtcacgag cagggcaagc tggagcaccg cttcgagtgg
      721 gggggcgacc tgggtgcccc cgacgagagc tacctctcgg cgcaattcga ccggccggtg
      781 atgatccacc gctacccggc ggcggtcaag gcgttttaca tggagccgga cccggcgcgg
      841 ccggagctcg cgctctgcgt cgacgtgctc gcgcccgagg gttatggcga gatcatcggc
      901 ggcggcgagc gcgcctcgtc gctcgacctg ctgctcgagc ggattcacga gcacgggctg
      961 ccggtcgagg ccttcgactg gtacctcgac ctgcgcaagt tcgggagcgt gccgcacgcc
     1021 ggtttcggca tgggcatcga gcgcgcggtc gcctggatct gcgggctcga gcatgtgcgc
     1081 gagacaatcc ccttcccgcg catgctctac cggctcaagc cctgacaaga tttcaaattt
     1141 gagatttgaa agtatgagcg ggatacctcc aaaacctggg gcgccgggcc accggccgcc
     1201 gccgatctcg cagtggcagg gcgagttcga gaagaaggtc gcgcaggagt tcgagcggcg
     1261 caccgggctc gactacaagg agacggtggc gctgatcatc aacgccatcg acccgttccc
     1321 ggggatgcaa gttttagacg tcgccaccgg gaccggggtc atcgcgcggc agatggtccg
     1381 gctggtcggc gagaaggggc gcatcgtcgg ggtcgacggg accgacaaaa tgatcgagca
     1441 ggcgcggctc gcggcgcagt cggccgggct cggccggcgg atcgagtgga aggtggccaa
     1501 ggccgagaag ctgcccttcg ccacagagag cttcgacctg atcacctgtg tgctcgcctt
     1561 ccacctgctc caggccgagc agttcctgga gcaggcgtgg cgcgtgctca agccgggggg
     1621 gcgcctcctg ctcgcgcacg agctggcgcc cagggggggg ctgagcccgc tgcggctgac
     1681 agcgcggcgc aactactatc agttcatcgc ccgcgacccg ggcgaggccg gcgcccactt
     1741 ctactcggcg gacgagatgg tcgcgatgct gctcgccgcc ggcttccgcc agtacgtcgt
     1801 ccgcggcctg cgcgagccca acaagttcga gctctcgttc tcgctgatca cggcggggaa
     1861 gtaaccatga ggaaagtgac gatcatcggc gtgctcatgg acctcggggc cgaccggcgc
     1921 ggggtcgaca tggggccgtc ggcgctgcgc gtcgcgagtc tcaacgagcg gctggccgag
     1981 ctcgggtgcg aggtgaccga cgccggcaac atcagcgtgc acaatccgga gatgctgccg
     2041 atcacggacc ggcggctgaa gtacctcccc gagatcgcgg cggcgagcca gcagttggcc
     2101 gaccgcgtcg aggcgatcct cgagggcggc tcgacgccaa tcgtcctcgg cggcgaccac
     2161 tcgatcgcca tcgggagcgt ggcggggctc gccgccttcc accggcggcg cgatgctcgc
     2221 gtcggcgtca tctggttcga cgcgcacggc gacatgaaca cgcccgagac ttcgccctcg
     2281 ggcaacatcc acggcatgcc cttcgccgcc atcctcgggc gcggggcgcc ggagttgacg
     2341 cacgtcagcg ggttcgcgcc caaggtctcg cccgaggact gcgtgctcat cggcgcgcgc
     2401 agcgtggacc gcgaggaggc ggccgcgctc cgggcctcgg gcatccgcgt cgtgaccatg
     2461 cgcgagctcg acgagcgcgg catgagcgcg gtcatggacg aggccatgtg gcttgcctcg
     2521 cgccgcaccg ccggctttca cgtgactatg gacatggact tcgtcgaccc ggacttcgcg
     2581 cctggcgtcg gcacgccagt cccgggcggc ccgacctacc gcgagagcca cctggcgatg
     2641 gagaagatcg ctgactcggg caagatgctc tcgttcgagc tgaccgaggt caacccggtc
     2701 ttggacgtct ccaaccggac ggccgagctc ggggtgcagc tgatcctttc ggcgttcggc
     2761 aaaaagatca tgtgacgttc gattcgggat ttgagatggg gaagttgagg gtcggggtca
     2821 ttttcggcgg gcgttccggg gagcacgagg tttcgctgcg gtcggccgag tcggtcat
//
LOCUS       DASRIG010000549         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_260337, whole genome shotgun sequence.
ACCESSION   DASRIG010000549 DASRIG010000000
VERSION     DASRIG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260337"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFB52_14680"
     CDS             <1..556
                     /locus_tag="VFB52_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZO07632.1"
                     /translation="DVAVAFFGEGTTNHGYFHECLNMAKVMGLPVVYFCENNRYGEFT
                     PFEDVTAGRIMDRPAALGVEAEQVDGMDVFAVREVAERAVQRAREGAGPQFVESLTYR
                     FVGHSRSDPAKYRKEGELDEWKQRDPLLVARERLAAAHGVEGPELDQVDEEVAALLAQ
                     MRAEGLAAPLPDPELLAAASEFKP"
     gene            553..1773
                     /locus_tag="VFB52_14685"
     CDS             553..1773
                     /locus_tag="VFB52_14685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZO07633.1"
                     /translation="MSTATAVEIPMPRLSDSMEEGTVLTWIKQVGDAVAVGEEIAEIE
                     TDKANMAFESDVAGTLLEIVVDEGETAAVGATIALIGDPGAEAPKPQAPAPEPEAAAP
                     APSANGGRVKASPLARRIARERGIDLVGLTGSGPSGRIVKADVEAAGPPTPTRQEGVG
                     GAAAGAGAPTAPETAKGAVETVELSKLQQVVARRMSESKATAPDFALSRELEMDAAVE
                     LRGRLKDWSGEGDVVPSYNDLVVKACALALQEHPRANGAYKDGRWELYERVNIGIAVA
                     AADGALIVPVVNDADKISLGQIARESRRLAGAAREGKVTPPELAGGTFTVSNLGMFGV
                     DNFTAVINPPQAAILAVGALAQKPVVVDGELAVGWRMGVTLVCDHRILSGADGAVFLA
                     RVAELLASPGALVL"
     gene            1799..>2223
                     /locus_tag="VFB52_14690"
     CDS             1799..>2223
                     /locus_tag="VFB52_14690"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZO07634.1"
                     /translation="MAEVEFREAIRIALDEELERDENVVLFGEDVAAPGGVFAVTPGL
                     FEKYGEQRVFDTPISELAITGAAFGSAVNGLRPVIEIMFGDFMPLAMDGLVNQAAKYW
                     FLSNEQQSGPLVVRSAIGAGGRFGAIHSQSPVSWFLGVPG"
BASE COUNT          324 a          802 c          783 g          314 t
ORIGIN      
        1 cgacgtcgcg gtcgccttct tcggcgaggg cacgaccaac cacggctact tccacgagtg
       61 cctgaacatg gccaaggtga tgggcctgcc ggtcgtctac ttctgtgaga acaaccgcta
      121 cggcgagttc acccccttcg aagacgtcac cgccggccgg atcatggacc gtcccgcggc
      181 gctcggcgtc gaggccgagc aggtcgacgg catggacgtc ttcgccgtcc gcgaggtcgc
      241 cgagcgggcc gtacagcgcg cccgcgaagg cgccggcccg cagttcgtcg agtcgctgac
      301 ctaccgcttc gtcggccact cccgctcgga cccggccaag taccgcaagg aaggcgagct
      361 cgacgagtgg aagcagcgcg acccgctgct ggtcgcccgc gagcgcctcg ccgccgcgca
      421 cggcgtcgag ggccccgagc tcgaccaggt cgacgaggag gtcgccgccc tgctcgccca
      481 gatgcgcgcc gagggcctcg ccgcgccgct gccggacccg gagctgctcg ccgcggcctc
      541 ggagttcaag ccttgagcac cgccaccgca gtcgagatcc cgatgccgcg cctctccgac
      601 tcgatggagg aggggaccgt gctgacctgg atcaagcagg tgggcgacgc cgttgcggtc
      661 ggcgaggaga tcgccgaaat cgagacggac aaggccaaca tggccttcga gtccgacgtc
      721 gccgggaccc tgctcgagat cgtcgtcgac gagggcgaga cggccgcggt gggggcgacg
      781 atcgccctga tcggtgaccc cggggccgag gcacccaagc cgcaggcccc cgcgcccgag
      841 cccgaagccg ccgcgcccgc tccctccgcc aacggaggtc gcgtcaaggc ttctccactg
      901 gcgcggcgga tcgctcgcga gcgtggcata gacctcgtcg gcctcacggg gtccggaccc
      961 tccggccgga tcgtcaaggc tgacgttgag gccgcgggtc ctcccacccc tacccgccag
     1021 gagggggtgg gaggagctgc ggccggcgct ggcgctccca cggctcccga gactgcgaag
     1081 ggcgcagtcg agacggtcga gctctcgaag ctccagcagg tcgtcgcgcg gcggatgtcg
     1141 gagtcgaaag cgacggctcc ggactttgcg ctctcgcgcg agcttgagat ggacgcggcg
     1201 gtcgagctgc gcgggcggct gaaggactgg agcggggagg gcgacgtcgt cccctcctac
     1261 aacgacctcg tggtcaaggc ctgcgcgctg gcgctgcagg agcacccgcg cgccaacggt
     1321 gcatataagg acggccgctg ggagctctac gagcgggtca acatcgggat cgccgtggcc
     1381 gccgccgacg gcgccctgat cgtcccggtc gtcaacgacg ccgacaagat ctcgctcggc
     1441 cagatcgcgc gtgagtcgcg ccgcctcgcc ggcgccgccc gcgagggcaa ggtgaccccg
     1501 cccgagctcg ccggcggcac cttcaccgtc tccaacctgg ggatgttcgg cgtcgacaac
     1561 ttcaccgcgg tgatcaaccc gccgcaggcc gcgatcctcg cggtcggcgc gctcgcccag
     1621 aagccggtcg tcgttgacgg cgagctggcg gtcggctggc gcatgggcgt gaccctggtc
     1681 tgcgaccacc gcatcctctc cggcgccgac ggcgccgtct tcctcgcccg cgtggcggag
     1741 ctgctcgctt ccccgggggc gctcgtactg tgaacaagct gcgaaaggag caccgctgat
     1801 ggctgaggtc gagttccgcg aggcgatccg gatcgccctg gacgaggagc tggagcgcga
     1861 cgagaacgtc gtcctcttcg gcgaggacgt ggccgccccc ggcggcgtct tcgcggtcac
     1921 gcccgggctg ttcgagaaat acggcgagca gcgcgtcttc gacacgccga tctccgagct
     1981 ggcgatcacc ggcgccgcct tcggtagcgc cgtcaacggc ctgcgcccgg tgatcgagat
     2041 catgttcggc gacttcatgc cgctggcgat ggacggcctc gtcaaccagg ccgccaagta
     2101 ctggttcctc tccaacgaac agcagagtgg gcccctggtc gtgcgctcgg cgatcggcgc
     2161 cggtggccgc ttcggcgcga tccactcgca gagcccggtc tcctggttcc tcggcgtgcc
     2221 cgg
//
LOCUS       DASRIG010000550         5962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2590116, whole genome shotgun sequence.
ACCESSION   DASRIG010000550 DASRIG010000000
VERSION     DASRIG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590116"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VFB52_14695"
     CDS             complement(<1..1432)
                     /locus_tag="VFB52_14695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZO07635.1"
                     /translation="MGEERVLLVSPVRDEASYIDDVVAGVESQTRPPALWVVVDDGST
                     DGTLEKLEAHAARLPFLKVVSTPPGYTVDSGDRLAAGGPDRAWNFGLRAAGGSAPFTH
                     LGKLDGDVVMPPEYLAGLLDRFRADPELGMAGGAIYEPDGSGGWVRLPTPPDHVTPMA
                     RLYTRACFEAIGGMPERLGADVITTTYAKLRGFRTATFADLPFRHLRHMGTAQGALRG
                     RARHGLYQWIVHYSLLWVALRSLLVALRFRPYGLSGLWFFAGYVGAALRGVPRVEDPE
                     FRAFVRAEQRTRLRRAIRRTPLASRLLGPATPPEVAVVAGIRKEVRPHTVDEVGETIL
                     QLEDYGNRSGWAGTDPYDGQNATRFAGPLRRSALGRRVLTQAVKRSPLDLRPLLGIAP
                     TRNAATVAWAVSAYARGAFLAPELQRDRLRATADLLVSLRLPDQEEPCWSYPFPIQTR
                     VLNYPPTAPNTISTAFAGMALLDAHEA"
     gene            complement(1434..2156)
                     /locus_tag="VFB52_14700"
     CDS             complement(1434..2156)
                     /locus_tag="VFB52_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07636.1"
                     /translation="MTPSTSPRPRRDPLALAALLVALLALVFSVTGLAGAGGPSSSQL
                     NKKIAQLNKRVSKLKVHCPISNSIDFGTWCLESTPHSVPVKDTGKNDYFYATRVCAQE
                     GGWLPTAAQLIGAARKAPLQSTIDDDPGTSGAEEFPEAIRGIKDKREMSSDLFTTTAG
                     SDAAGSEGVTAGSKGNGAQGEPDPIPMPADPVPDTLNYVTVYDNHNRGGFAGGVPVGK
                     PESFRCAYAKTSQSKQRISRTG"
     gene            complement(2153..>5962)
                     /locus_tag="VFB52_14705"
     CDS             complement(2153..>5962)
                     /locus_tag="VFB52_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07637.1"
                     /translation="GCTPPLVGFQVVAIDAAAGDAWLLGQGATLGEGLELFHRETGGG
                     SPVWRQVPLGPPNTLGARYGQAEADGIQIAARSQGQPLTASASGVWIDATLDGAEDAT
                     VYFDAGKGEVTGAWCDLGSVALCPLPLGGELPSGQGRSFAWPADSANPFGTRSVTGVG
                     QGAILTFAGSSFRRLTFVGGTAGASQGAALSAPDEGWLGATPPLQLTRNPAAARLQSW
                     PVPFRRPLTALATAPGSTPGALDSEALAVGAEGQVARYVPGIGWEPEFLLRASGKRAT
                     PRLRAVAWPEEERAYAVGDNGNFWRWQKKTGLWQPDPGAPANMTRGNMTGVAFDPAKP
                     SRGYAIGQQGVLLGYGREWKQEALPPGVPAEANLTSIAFAGSEALVTWKFPENPNNSG
                     TPRYFGGVIVNDGFGWRTEPLAEAALTESEGRAVPQRVAGLPDGGAVIATLGFGESTP
                     PVEGRVIERQGPGAAWQVAPGGAVGYPAALAAIREGGQVRAIVSVTPKAGVSQGSAEM
                     TIDQEQIFNQPPAGQAPLLTDPYPLPATGVVMRQTPGGWRDEQQQNYPLPQSNLGQSL
                     YDLPLRPDPVLALLITADGSGGWAVGGETGSATKYRRGEIVTAGVMRYGSGAAPPSNT
                     TAAPISAPSGVATFALGAHAQCVAQCADYGGAGLGPDRWLRAAVSRAASVPGMRAFLY
                     AGNSVAPTQGAPQSEQTLAQVIGAGAYAREQAAYASRLGNGGGALPVFAAPSASDLDA
                     SGSLATFYSAFAGFGAPFGTSSARPGIAPVSQGNSAQPYYAFDSTGAGGAVRVVVLDY
                     SRPELGDGQRCWLAQQLSGAAAVGRPAIVVGGRDLAGLAPDAAADRTQVIQTLVGVGA
                     PAGCGVGAASAYLFDFPEENRAYRLSAGGRSIPAYGSGTLGYVTPPAPTDTDFAGASG
                     FLTVSVDVANRDKDTNVAPVAVQLVPSIGSLALDATDGTLLRRSQPALFEGLARRPLA
                     GSKCEGDAAAPAVCDFQSPPPYVPVPSDCLGTNCATSVFPEYTFSSSRPDIADFVSAD
                     PGSTNPRNVLLVKGKPVLDPHSGLLCAFNAGTTLVSITTGGLAYTQKVTVQAGTVQRP
                     CGTTPLKKEEGDAEDPGEPQAPKPEVPVELPIETVPPPPPPPAPQVQPSPPPAAQPIP
                     PTPTAPIAYVPIPISPAVPLVPIVPPPPLPAVQPTPPSGTSQVNAVEEEEEPEEATES
                     SSAFAALPEPGPANVSLRRRDSDGGVPLLIPALVLVAAVAAAGIGGRRRRDPRPAFQT
                     NNNRRYR"
BASE COUNT          783 a         2011 c         2356 g          812 t
ORIGIN      
        1 tcgcctcgtg ggcgtcgagc agcgccatcc cggcgaaggc ggtggagatc gtgttcggcg
       61 cggtcggcgg gtagttgagc acccgggtct ggatcgggaa cgggtagctc cagcagggct
      121 cctcttggtc gggcaggcgc agcgagacca agaggtcggc ggtagcgcgc agccggtcgc
      181 gctgcaactc gggcgcgagg aaggcgccac gggcgtaggc ggagacggcc caggcgacgg
      241 tggcggcgtt gcgggtcggc gcgatcccga gcagcggccg caggtcgagc ggcgagcgct
      301 tgaccgcctg ggtgagcacg cgccggccga gcgcgctgcg ccgcagcggg ccggcgaagc
      361 gggtcgcgtt ctggccgtca taggggtcgg ttccggccca gccgctgcgg ttgccgtagt
      421 cctcgagctg caaaatcgtc tcccccacct cgtccaccgt gtgaggtcgc acttctttcc
      481 ggataccggc aacaactgcg acctcagggg gggtggccgg tccgaggagg cggctggcca
      541 ggggagtgcg acggatggcg cggcgcaggc gggtgcgctg ctcggcgcgg acgaaggcgc
      601 ggaactcggg gtcctcgacc cgcggcacgc cgcgcagagc ggcgccgacg tagccggcga
      661 agaaccagag gccggagagg ccgtaggggc ggaagcgcag cgcgaccagc agcgagcgca
      721 gcgccaccca gagcagcgag tagtggacga tccactggta gaggccgtgg cgggcgcggc
      781 cgcgcagcgc tccctgggcg gtgcccatgt ggcgcaggtg gcggaacggg aggtcggcga
      841 aggtggcggt gcggaagccg cgcagcttcg cgtaggtggt cgtgatcacg tcggcaccga
      901 gccgttcggg catgccgccg atcgcctcga agcaggcgcg ggtatagagg cgggccatcg
      961 gggtgacgtg gtcgggcggg gtcggcagcc gcacccagcc gccgctgccg tcgggctcgt
     1021 agatcgcgcc gccggccatc cccagctcgg gatcggcgcg aaagcggtcg agcaggcccg
     1081 cgaggtactc gggcggcatc accacgtcgc cgtcgagctt gcccaggtgg gtgaagggcg
     1141 ccgagccgcc ggccgcgcgc aggccgaagt tccaggcgcg atccggcccc ccggcggcga
     1201 gccggtcgcc gctgtcgacc gtgtaccccg gcggggtcga gacgaccttc aggaagggca
     1261 ggcgggcggc gtgcgcttca agcttctcca gcgtgccgtc ggtcgagccg tcgtcgacga
     1321 cgacccagag ggccggtggc cgggtctggg actccacacc ggccaccacg tcgtcgatgt
     1381 aggaggcctc gtcgcggacc ggcgagacca gcagtacgcg ctcctcgccc atctcagccg
     1441 gtgcgcgaga tccgctgctt cgactgcgag gtcttcgcgt aggcgcagcg gaaggactcg
     1501 ggcttgccga ccgggacgcc gccggcgaag ccgccgcggt tgtggttgtc gtagacggtc
     1561 acgtagttga gcgtgtccgg gaccgggtcg gccggcatcg ggatcgggtc cggctcgccc
     1621 tgcgcgccgt tgcctttcga gcccgcggtg accccctccg agccggccgc gtcggagccg
     1681 gcggtggtcg tgaacaggtc gctgctcatc tcgcgcttgt ccttgatccc gcggatcgct
     1741 tccgggaatt cttcggcgcc cgaggtcccc gggtcgtcgt cgatcgtgct ctgcagcggc
     1801 gccttgcggg cggcgccgat cagctgcgct gcggtcggca gccagccgcc ttcctgggcg
     1861 cagacccggg tcgcgtagaa gtagtcgttc ttgccggtgt ctttgaccgg gaccgagtgc
     1921 ggggtcgatt ccaggcacca ggtgccgaag tcgatcgagt tcgagatcgg gcagtggacc
     1981 ttcagcttcg agacccgttt gttcagctgc gcgatcttct tgttgagctg ggagctggac
     2041 gggccaccgg ccccggcgag accggtgacc gagaagacga gggcgagcag ggcgaccagc
     2101 agcgccgcca gcgccagcgg gtcgcggcgc ggacggggcg aagtcgaggg ggtcatcggt
     2161 acctccggtt gttgttcgtc tggaaggctg ggcgggggtc gcgccggcgc cggccgccga
     2221 tcccggcggc ggcgaccgcg gcgacgagca cgagcgccgg gatcagcagc ggcaccccgc
     2281 cgtccgagtc gcgccggcgc agggagacgt tcgcgggccc gggctcgggc agcgcggcga
     2341 aggccgagga cgattcggtc gcctcctccg gctcctcttc ttcctcgacc gcgttcacct
     2401 gcgaggtgcc gctcggcggg gtcggctgca ccgcgggcag cggcggcggg ggcacgatcg
     2461 ggaccagcgg caccgccggg gagatcggga tcgggacgta ggcgatcggc gccgttgggg
     2521 tcggtgggat cggttgcgcg gccggcgggg gcgacggctg gacttgcggg gcgggaggcg
     2581 gcgggggcgg cggaacggtt tcgatcggca gttcgaccgg cacttccggt ttcggcgctt
     2641 gcggctcacc ggggtcttcg gcgtcgcctt cttctttttt cagcggcgtc gtaccgcagg
     2701 gccgctgcac ggttccggcc tggaccgtca ccttctgggt gtaggcgagg cccccggtgg
     2761 tgatcgagac gagggtggtg ccggcgttga aagcgcagag caggcccgag tgcggatcga
     2821 gcacaggctt gcctttgacc aggagcacgt tgcgcgggtt ggttgagccg gggtcggcgg
     2881 agacgaagtc ggcgatgtcc gggcgcgagg agctgaaggt gtactcgggg aagacgctgg
     2941 tcgcgcagtt ggtgcccagg cagtcgctcg ggaccggcac gtacgggggt gggctctgga
     3001 agtcacagac cgccggcgcc gcggcgtcgc cttcgcactt cgagccggcc agcggtcggc
     3061 gggcgaggcc ttcgaagagg gcgggctggc tgcggcgcag cagcgtcccg tcggtcgcgt
     3121 cgagcgccag cgagccgatc gagggcacga gctggacggc gaccggggcg acgttggtgt
     3181 ccttgtcgcg gttggcgacg tcgaccgaga cggtgaggaa gccggaggcg ccggcgaagt
     3241 cggtgtcggt cggcgccggc ggagtcacgt agccgagcgt gccgctgccg taggcgggta
     3301 tcgagcgacc gccggcgctg agccggtaag cgcgattctc ttcggggaag tcgaagaggt
     3361 aggccgaggc cgcccctacc ccgcatccag ccggggcgcc gaccccgacc agggtctgga
     3421 tcacctgcgt gcggtcggcg gcggcatccg gggcgagccc ggcgaggtcg cggccaccga
     3481 cgacgatcgc tggccgaccc acggcggcgg ccccggagag ctgctgggcg agccagcagc
     3541 gctggccgtc gccgagctcg ggtcgcgagt agtcgagcac gacgacgcgg accgcgccac
     3601 ccgcgccggt cgagtcgaag gcgtagtagg gctgggcgga gttgccctgc gagaccggcg
     3661 cgatccccgg ccgcgcggag gaagtgccga agggcgcacc gaagccggcg aaggccgagt
     3721 agaacgtcgc gagcgagccg ctggcgtcga ggtcggaggc ggacggcgcc gcgaacaccg
     3781 gcagcgcccc gccgccgttg ccgagccggc tcgcgtaggc agcctgctca cgggcgtagg
     3841 cgccggcgcc gatcacctgg gcgagggtct gttcgctctg cggggccccc tgggtggggg
     3901 cgacgctgtt gccggcgtag aggaacgccc gcatccccgg cacgctcgcc gcgcgcgaga
     3961 ccgccgcccg cagccagcgg tcgggaccga gcccggcgcc gccgtagtcg gcgcactggg
     4021 caacgcactg ggcgtgggcg ccgagcgcga aggtcgcgac cccggagggc gccgagatcg
     4081 gcgccgccgt cgtgttcgag ggcggcgcgg cgccggaccc gtagcgcatc accccggcgg
     4141 tgacgatctc cccgcggcgg tatttggtcg ccgacccggt ttcaccgccg accgcccagc
     4201 caccgctgcc gtcggcggtg atcagcaggg cgaggaccgg gtcggggcgc agcggcaggt
     4261 cgtagagcga ctgcccgagg ttgctctgcg gcagcgggta gttctgctgc tgttcgtcgc
     4321 gccagccacc gggggtctgc cgcatcacca cgccggttgc cggcagcggg tagggatcgg
     4381 tcagcagcgg cgcctgaccg gccggcggct ggttgaagat ctgttcctgg tcgatggtca
     4441 tttccgcgct gccctggctg acaccggctt tcggagtgac cgagacgatc gcccgcacct
     4501 gcccgccttc gcgaatcgcc gccagcgccg ccgggtagcc gaccgcgccg ccgggggcga
     4561 cctgccaggc ggcacccggg ccctggcgct cgatcacccg cccctcgacc ggcggcgtgc
     4621 tttcgccgaa cccgagcgtg gcgatcacgg cgccgccatc gggcagcccg gcaacccgct
     4681 gcggaaccgc tcggccctcg gactcggtaa gtgcggcctc ggccagcggt tcggtgcgcc
     4741 agccaaagcc gtcgttgacg atcacgccac cgaagtaacg cggggtgccg gagttgttgg
     4801 ggttttccgg gaatttccag gtgaccagcg cttctgagcc ggcgaaggcg atcgaagtca
     4861 ggttcgcttc cgccggcacc ccgggcggca gcgcttcctg cttccactcg cgcccgtagc
     4921 cgagcaaaac gccctgctgg ccaatcgcgt agccacgact cggcttcgcc gggtcgaagg
     4981 cgaccccggt catgttgccg cgggtcatgt tcgccggtgc accggggtcg ggctgccaga
     5041 ggccggtttt cttttgccag cgccagaagt tgccgttgtc gccgaccgcg taggcgcgtt
     5101 cctcttcagg ccaggcgacc gcacgcaggc gcggggtcgc ccgtttgccg gaggcgcgca
     5161 ggaggaattc cggttcccag ccgattcccg gcacgtagcg cgcgacctga ccttcggccc
     5221 cgaccgcgag cgcttcggag tcgagcgcgc cgggggtcga tccgggcgcg gtcgcgagcg
     5281 cggtcagcgg ccgccggaag gggaccggcc acgactgcag gcgcgccgcc gccgggttgc
     5341 gggtcaactg cagcggcggc gtcgccccca gccaaccttc gtcgggagcg ctcagcgccg
     5401 cgccctggct ggccccggcg gtgccgccga cgaaggtgag acggcggaag gaactgccgg
     5461 cgaaggtgag gatcgcgccc tggccgacgc cggtcaccga gcgggtcccg aaggggttgg
     5521 cggagtcggc cggccaggcg aagctgcgcc cctggccgga gggcaactcg ccgccgagcg
     5581 gcagcgggca gagcgccacc gagccgaggt cgcaccaggc accggtgact tcgcctttgc
     5641 cagcgtcgaa gtagacggtg gcgtcctcag cgccgtcgag ggtcgcgtcg atccagaccc
     5701 cgctcgcgct cgcggtcaag ggctggccct ggctgcgggc cgcgatctgg attccgtcgg
     5761 cttcggcctg gccgtagcgg gcgccgagcg tgttcggcgg gccgagcggc acctggcgcc
     5821 agacgggcga cccgccaccg gtttcgcggt ggaacagctc cagcccctca cccagcgttg
     5881 cgccctgccc gagcagccag gcgtcgccgg ccgcggcgtc gatcgcgacg acctggaagc
     5941 cgaccagggg aggcgtgcac cc
//
LOCUS       DASRIG010000551         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1028084, whole genome shotgun sequence.
ACCESSION   DASRIG010000551 DASRIG010000000
VERSION     DASRIG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028084"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VFB52_14710"
     CDS             <1..1536
                     /locus_tag="VFB52_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZO07638.1"
                     /translation="EGAFAYPENQRLTYRRADELANRCANAMLARGLERGDRVLLYCE
                     NSVEAYVAKIAIAKAGLVSAPINPMLAPDVVTHLIERVEPKFAIVDDELWPKIESAFG
                     EAGLEPGVTIPIEGWTVPGTVEFSEFIADASAEEPEVEIHGDDIWEIIFTSGTTAMPK
                     GSMVSHHYSYFGAYSFALTLTRGIRFECDLKLVAFLPLIYHIADQIFSLPAWLCGGTL
                     IIGRKHDPAEIARALSDEQATALWSGSPAMLEELAKELEAAPELDASELKTAIFGWTA
                     IPPELVDRLKRLTSPDLNVCEILGQTEAISCFRFWPDKWPEIFAEKAPAMNYVGVPNP
                     LLASKVVDEEGNSLEGTVGEPGEVVYRSPVVTPGYYLDEDATREAFRDGWFHSGDVCM
                     YDEEGLRIMVDRSKDMIKSGGENVSTLRVETILRSHPEILKAAVVGLPHERWGEAVTA
                     IIVADEGGDPDPDEVMAFCRERLAGYESPKGVMVVDDLPETVGGKVLKYKLRVAHAGF
                     YES"
     gene            1561..2517
                     /locus_tag="VFB52_14715"
     CDS             1561..2517
                     /locus_tag="VFB52_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZO07639.1"
                     /translation="MDSPELRFDDQVAIVTGAGRGLGRAYALALAERGAKVVVNDLGG
                     SVAGEGSDPAPAEEVAAEIRAAGGEAVSSDADVADEAAAASIVALAAESFGGVHIVVN
                     NAGNMDPCPPEELRAADLERHLSIHATGTLNVTAAAWPALVEQRYGRVISTTSIGLFG
                     GAFIAAYAAAKGAAFSLGRSLAEAGAEHGIRVNNLAPAAETRMVLDPELRAKCGLPPL
                     PDDAEPDPARGVDAVVPMLLALAHETCPANGETLIAGLGRFARIFAAETPGLVQPGLS
                     PEELLDRWEEVVAEDGYEVEMSTAEAAAYRQPLIDAALERLA"
     gene            complement(2524..2961)
                     /locus_tag="VFB52_14720"
     CDS             complement(2524..2961)
                     /locus_tag="VFB52_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HZO07640.1"
                     /translation="MPAPPLSATAEELMLAPNPAVLATVRPDGTPHSAAVWFDWVAGR
                     VLLNMDVNRRRLRFIEANPAASLTVLDGENWFRQLSVEGPITLEPDEGLVTVDRLARR
                     YIDAEYPIRDRERVTGWLDPTRWFLWDARGRGDEALARRPKLG"
     gene            complement(2966..3787)
                     /locus_tag="VFB52_14725"
     CDS             complement(2966..3787)
                     /locus_tag="VFB52_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07641.1"
                     /translation="MSATAELTGHYVEIDGNRTFYDECGEGTPVVCVHTAGADSREYQ
                     YLLPELAARGYRGLALDLPGHSRSYPVDWEPHRSINQHAEYVWRFAQEVCGGEKPVMI
                     GCSIGGCIAFDLAANHSADFRAIVPMEGLAWGSPILPHPAELERPAWSTAWKPFLEYA
                     AVESLGKPTLADEEKVKELWWQHQNAQQAGNGDIQGWAGHDVRDKLGDVRCPVLVVKG
                     ADDFWVPELLINYSAELIGDQAEVAILPDIGHYPMFEDPEGLAELVDEFVQRRAA"
     gene            complement(3784..>4014)
                     /locus_tag="VFB52_14730"
     CDS             complement(3784..>4014)
                     /locus_tag="VFB52_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07642.1"
                     /translation="SGERTFTDLVSAGSRYAESIEEIEAGGGGYRPPLLAIEGNGVEA
                     GRMHLATMYLARAFAQTAVESGALAGEAGVAA"
BASE COUNT          575 a         1464 c         1409 g          566 t
ORIGIN      
        1 gagggcgcct tcgcctaccc cgagaaccag cggctcacct accgccgcgc cgacgagctc
       61 gccaaccgct gcgccaacgc gatgctggcc cgcggcctcg agcgcggcga ccgggtgctg
      121 ctctactgcg agaactcggt cgaggcctac gtcgccaaga tcgcgatcgc caaggccggc
      181 ctggtctcgg cgccgatcaa cccgatgctg gcgcccgacg tcgtcaccca cctgatcgaa
      241 cgggtcgaac cgaagttcgc gatcgtcgac gacgagctct ggccgaaaat cgagtcggcc
      301 ttcggcgagg ccgggctgga gccgggcgtg acgatcccga tcgagggctg gacggtgccg
      361 ggcacggtcg agttctccga gttcatcgcc gacgcctcgg ccgaggagcc cgaggtcgag
      421 atccacggcg acgacatctg ggagatcatc ttcacctcgg ggacgaccgc gatgccgaag
      481 ggctcgatgg tctcccacca ctactcctac ttcggcgcct actcgttcgc gctcaccctg
      541 acccgcggga tccgcttcga gtgcgacctc aagctggtcg ccttcctgcc gctgatctac
      601 cacatcgccg accagatctt ctcgttgccg gcctggctct gcggcggcac cctgatcatc
      661 ggccgcaaac acgatccggc cgagatcgcg cgggcgctca gcgacgagca ggcgacggcg
      721 ctgtggagcg gctcgccggc gatgctcgag gagctggcga aggagctgga agcggcgccc
      781 gagctcgacg cctccgaact gaagacggcg atcttcggct ggaccgcgat cccgccggag
      841 ctggtcgacc ggctgaagag gctgaccagc ccggacctca acgtctgcga gatcctcggc
      901 cagaccgagg cgatctcctg cttccgcttc tggcccgaca agtggccgga gatcttcgcc
      961 gagaaggcgc cggcgatgaa ctacgtcggc gtgcccaacc cgctgctggc ctcgaaagtg
     1021 gtcgacgagg agggcaactc gctggagggc acggtcggcg agccgggcga ggtggtctac
     1081 cgctctccgg tcgtcacccc cggctactac ctcgacgagg acgccacccg cgaggccttc
     1141 cgcgacggct ggttccactc cggcgacgtc tgcatgtacg acgaggaggg gctgcggatc
     1201 atggtcgacc gctccaagga catgatcaag tccggcggcg agaacgtctc caccctgcgg
     1261 gtcgagacga tcctgcgctc gcaccccgag attctcaagg ccgccgtcgt cggcctcccg
     1321 cacgagcgct ggggcgaggc ggtgaccgcg atcatcgtcg ccgacgaggg cggggacccc
     1381 gatcccgacg aggtgatggc gttctgccgc gagcgcctcg ccggctacga gtcgccgaag
     1441 ggcgtgatgg tcgtcgacga tctccccgag acggtcggcg gcaaggtgct gaagtacaaa
     1501 cttcgggtcg cccacgcggg cttctacgag agctaggaaa gcgacagaag gaggcggttc
     1561 gtggactccc cggaactgcg tttcgacgac caggtggcga tcgtcaccgg tgccggccgc
     1621 ggccttggcc gcgcctacgc cctggcgctg gccgagcgcg gcgcgaaggt ggtcgtcaac
     1681 gacctcggcg gcagcgtcgc gggcgagggc tccgacccgg ccccggccga ggaagtcgcg
     1741 gcggagatcc gcgcggccgg cggcgaggcg gtgtccagcg acgccgacgt cgccgacgag
     1801 gccgcggcgg cctcgatcgt cgcgctcgcg gccgagagct tcggcggggt ccatatcgtc
     1861 gtcaacaacg ccggcaacat ggacccctgc ccgccggagg agttgcgcgc cgccgacctc
     1921 gaacggcacc tctcgatcca cgcgaccggc acgctcaacg tgaccgcggc ggcctggccg
     1981 gcgctggtcg agcagcgcta cggacgggtg atctcgacga cctcgatcgg ccttttcggc
     2041 ggcgccttca tcgccgccta cgcggccgcc aagggagcgg ccttctcgct cggccgctcg
     2101 ctcgccgagg ccggcgccga gcacggcatc cgcgtcaaca acctcgcccc cgcggcggag
     2161 acgcggatgg tcctcgaccc cgagctgcgc gccaagtgcg gcctgccgcc gctgcccgac
     2221 gacgccgagc ccgaccccgc ccgcggcgtc gacgcggtcg tgccgatgct gctggccctc
     2281 gcccacgaga cctgccccgc caacggcgag accctgatcg ccgggctcgg ccgcttcgcc
     2341 cgcatcttcg ccgccgagac ccccgggctc gtccagcccg ggctctcccc cgaggagctg
     2401 ctcgaccgct gggaggaggt cgtcgccgag gacggctacg aggtcgagat gtcgaccgcc
     2461 gaagccgccg cctaccgcca gccgctgatc gacgccgcgc tcgagcgcct cgcctgaggc
     2521 ggctcagccg agcttcgggc ggcgggccag ggcctcgtcg ccgcggccgc gggcgtccca
     2581 gaggaaccag cgggtcggat cgagccagcc ggtaacccgc tcgcggtcgc ggatcgggta
     2641 ctcggcgtcg atgtagcgcc gggcgaggcg gtcgacggtg accaggccct cgtcgggttc
     2701 gagcgtgatc ggcccctcga cgctgagctg gcggaaccag ttctccccgt cgaggacggt
     2761 gagcgacgcc gccgggttcg cttcgatgaa gcgcaggcgg cgccggttca cgtccatgtt
     2821 cagcagcacc cgtcccgcca cccagtcgaa ccagaccgcc gcgctgtgcg gggtgccgtc
     2881 ggggcgcacc gtcgccagca cggcggggtt cggggcgagc atcagctcct cggcggtggc
     2941 cgagagcggc ggcgcgggca tcgtcctagg ccgccctccg ctggacgaac tcgtcgacca
     3001 gctcggccag gccctcgggg tcctcgaaca tcgggtagtg gccgatgtcg ggcaggatcg
     3061 ccacctcggc ctggtcgccg atcagctcgg ccgagtagtt gatcagcagc tccgggaccc
     3121 agaagtcgtc ggcgcctttg accaccagca ccgggcaccg gacgtcgccg agcttgtcgc
     3181 gcacgtcgtg gccggcccag ccctggatgt cgccgttgcc ggcctgttgg gcgttctggt
     3241 gctgccacca gagctccttc accttctcct cgtcggccag ggtcggcttg ccgagcgact
     3301 cgaccgcggc gtactcgagg aacggcttcc aggcggtcga ccaggccggc cgctccagct
     3361 ccgccgggtg cggcaggatc ggcgagcccc aggcgaggcc ctccatcggc acgatcgcgc
     3421 ggaagtcggc gctgtggttg gcggcgaggt cgaaggcgat gcagcccccg atcgagcagc
     3481 cgatcatcac cggcttctcg cccccgcaga cctcctgggc gaagcgccac acgtactcgg
     3541 cgtgctggtt gatcgagcgg tgcggttccc agtcgaccgg gtaggagcgc gagtggccgg
     3601 gcaggtcgag ggcgagcccg cggtatccgc gcgccgccag ctccggcagc aggtactggt
     3661 actcgcgcga gtcggcgccg gcggtgtgca cgcagacgac cggcgtgccc tcgccgcact
     3721 cgtcgtagaa ggtgcggttg ccgtcgatct cgacgtagtg gccggtgagc tcggcggtgg
     3781 cgctcatgcc gccacccccg cctcgcccgc gagcgccccg gactcgaccg ccgtctgcgc
     3841 gaaggcgcgg gccaggtaca tcgtcgctag gtgcatgcgg ccggcttcga ccccgttgcc
     3901 ctcgatcgcc agcagcggcg gccggtagcc gcccccgccc gcttcgatct cctcgatcga
     3961 ctcggcgtaa cgcgagccgg ccgagaccag gtcggtgaag gtgcgctcgc cgct
//
LOCUS       DASRIG010000552         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2590844, whole genome shotgun sequence.
ACCESSION   DASRIG010000552 DASRIG010000000
VERSION     DASRIG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590844"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFB52_14735"
     CDS             complement(<1..380)
                     /locus_tag="VFB52_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07643.1"
                     /translation="MDELKKLLAERACERLINEYARRVDFGEAAQIADLFTEDARWEG
                     DQVFAGRDKIREWFEGRQGVTRRVSRHVCTNVMVEVLSPTEARSHCYMINYRHDRAEG
                     DDSLPVPTEVPKYVGELHDTFRLT"
     gene            complement(449..1702)
                     /locus_tag="VFB52_14740"
     CDS             complement(449..1702)
                     /locus_tag="VFB52_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZO07644.1"
                     /translation="MALLLVALLAMFVVACGGDDDSSSSGGETATTEETGGGEESGAL
                     LEPTETAANASYLYGDTELGGGIHPGEGGDELAEKSIKAGEEAGEEAGKATLPSATIG
                     VINFLNGIESSDRLADSVTLAAGELNWKTIVCDGKGTPSQFVACGNSLLAQGVDGILA
                     IAIEPGQITSVVAKANSAGVPIAQIGGGGVPLGDYNGNYGPNEAKAGELLTEGIFEKL
                     DPLEGNPEIIVHNFPAKWGSERTEQLEKAVSEQDKIKIADNVVTDAADLVPFTERTVA
                     TQITQYPDAAAYWFTFDTTGQVGGGVISGKYPGKEFPDKPLVATFHGDQATLGLIAQG
                     DIDLISDVNYDASSWMGIDGIATELGQKKPMSTENQPKYPVIGDAFTYVNIDKENLPP
                     EGQYMPTEWDVPAYFISKWKAEFGK"
     gene            complement(1797..3344)
                     /locus_tag="VFB52_14745"
     CDS             complement(1797..3344)
                     /locus_tag="VFB52_14745"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZO07645.1"
                     /translation="MNAVTAPLKIEARGLVKQYPGVRALNGADLTIEGGRIHGLLGKN
                     GAGKSTVIKVLAGAVHPDEGEILVNGEPTSIPNPHAASALGFAFAHQELMDVPNLTVA
                     ENVLLGLGYPTVAGSLVRTRAMRKKARQALDRLEADINPAAPLTSLSVAERRLVVIAH
                     GLAADARMIVLDEPTASLTDSEIEHLDKVLRSLRNDGVAIVYVSHRLDEIFNLTDQVT
                     VFRDGANVFEGETKDLSRAELIDRITGEKISVDVEARTPHFAAPDAKELMRVENMTLP
                     GVVEDAGFTLYEGEMLGIAGLVGSGRTELMRMIFGADKSSEGEVYLRGEKQKIRSPRD
                     GIEAGVVLLPEDRKGHGGVFTFSVRKNITLPSMPKFRLVGPAPIPSTRQEKAAARELV
                     ERLEIKVSNVEHPARHLSGGNQQKMVLAKWLDTGADVFIFDEPTQGIDIGGKEDVYRL
                     MTGLAERGKGVIFISSEFTELVGTCGRVLVMREGRLVAEFKGDSVTEETLVGACYEHE
                     PAAANGA"
     gene            complement(3344..>3706)
                     /locus_tag="VFB52_14750"
     CDS             complement(3344..>3706)
                     /locus_tag="VFB52_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZO07646.1"
                     /translation="GNEEAARLSGLRVRPYRVIGFIAVGLAAAIVGILMISLSGSYSP
                     NGGATYLLPAFAAAFLGAAVFRPGEFNIPGTVVGVLFLGVIQTGLVMLNTESYVINLV
                     QGGILIAAVLVSRLGQRA"
BASE COUNT          546 a         1197 c         1242 g          721 t
ORIGIN      
        1 tcggtcagcc ggaaggtgtc gtgcagctcg ccgacgtatt tcggcacttc ggtcgggacc
       61 ggcagcgagt cgtcgccttc ggcgcggtca tgccggtaat tgatcatgta gcagtggctg
      121 cgggcctcgg tcggggagag cacctcgacc atcacgttgg tgcagacatg gcgcgagacc
      181 cgccgggtca ccccctggcg tccctcgaac cactcgcgaa tcttgtcccg gcccgcgaag
      241 acctggtccc cctcccagcg cgcgtcctcg gtgaagaggt cggcgatctg cgcggcctca
      301 ccgaagtcga cgcggcgcgc gtactcgttg atcagccgtt cgcaggcgcg ctcggcgagg
      361 agtttcttca gctcgtccat atatatggag cctcaaagca tgacgccgcc gggccagggg
      421 cccggcggcg tcagcgatcc ggttgtgcct atttgccgaa ttcggctttc cacttcgaga
      481 tgaagtaggc gggcacgtcc cattcggtcg gcatgtattg gccttccggg gggaggtttt
      541 ctttgtcgat gttcacgtag gtgaacgcgt caccgatcac cgggtatttc ggctggtttt
      601 ccgtgctcat cggttttttc tgaccgagtt cggtcgcgat cccgtcgatg cccatccacg
      661 acgaagcgtc gtagttgacg tcggagatca ggtcgatgtc gccctgggcg atcagaccca
      721 gcgtcgcctg gtcaccgtgg aacgtcgcga ccagcggctt gtccgggaat tctttgcccg
      781 ggtatttgcc cgagatcaca ccgccgccga cctggccggt ggtgtcaaac gtgaaccagt
      841 acgccgcggc gtcggggtac tgcgtgatct gcgtggcgac cgtccgttcg gtgaacggaa
      901 caaggtcggc cgcgtcggtc acgacgttgt cggcgatctt gatcttgtcc tgttcgctga
      961 cagctttttc aagctgttcg gtccgttccg agccccattt ggccgggaag ttgtggacga
     1021 tgatctccgg gttgccttcg agcggatcga gcttttcgaa gatgccttcg gtcagcagtt
     1081 cgccggcttt cgcctcgttc gggccgtagt tgccgttgta gtcgccgagc ggaacaccgc
     1141 cgccgccgat ctgggcgatc gggacgccgg ccgagttggc tttggcgacg accgacgtga
     1201 tctgaccggg ttcgatcgcg atcgcgagga tcccgtcgac gccctgggcg agcagcgagt
     1261 tgccgcaggc gacgaactgg gacggcgtgc ctttgccgtc gcagacgatc gtcttccagt
     1321 tgagttcgcc agccgccagc gtcacagaat cggccagacg gtcggaggat tcaattccgt
     1381 tgaggaaatt gatgactccg atcgtggccg acgggagcgt cgctttcccc gcttcttcac
     1441 cggcttcttc accggctttg atcgattttt cggcgagttc gtcgccacct tcgcccgggt
     1501 ggataccgcc cccgagttcg gtgtcgccgt acaggtagct cgcgttggct gcggtttcgg
     1561 tcggttcgag cagcgcgccg ctttcttcgc cgccgccggt ttcttcagtg gtagcggttt
     1621 caccgccgct gctgctgctg tcgtcgtcgc caccacatgc gacgacaaac atcgccagca
     1681 gcgcgaccaa gaggagagcc aatagctgcc tccccctcac gtttccgaat gcactcatac
     1741 ttgttgtctc ctcctagttg gcagtaccac ggtgaaacgt ctgtacgtcg aagccgtcag
     1801 gctccgtttg ccgcggccgg ctcgtgctcg tagcaagcgc cgaccaaggt ctcctcggtg
     1861 accgaatccc ccttgaactc cgccaccagc cggccctcgc gcatgaccag tactcggcca
     1921 cacgttccga ccagctcggt gaactccgac gagatgaaga tcaccccttt gccccgttcc
     1981 gcgaggccgg tcatcaggcg gtaaacgtcc tccttgccgc cgatgtcgat gccctgggtg
     2041 ggctcgtcga agatgaatac gtcggcaccg gtgtccagcc acttggccag caccatcttc
     2101 tgctggttgc cgccggagag gtggcgggcc gggtgctcga cgttggaaac cttgatctcc
     2161 agccgttcga ccagctcgcg cgcggccgcc ttctcctggc gcgtgctggg aatcggcgcc
     2221 ggcccgacca gccggaattt cggcatcgag ggcagggtga tgtttttgcg caccgagaag
     2281 gtgaagacgc cgccgtggcc tttgcggtcc tcgggcagca gcacgacccc ggcctcgatc
     2341 ccgtcgcgcg ggctgcggat cttctgcttc tcgccgcgca ggtagacctc gccttcggag
     2401 gatttgtcgg cgccgaagat catccgcatc agctccgtgc ggccggagcc gacgaggccg
     2461 gcgatcccca gcatctcgcc ctcgtacagg gtgaagccgg cgtcctcgac gacgcccggc
     2521 agggtcatgt tctcgacccg catcagctcc ttcgcgtcgg gggcggcgaa gtgcggggtg
     2581 cgggcctcga cgtcgaccga gatcttctcg ccggtgatcc ggtcgatcag ctcggcccgc
     2641 gagaggtctt tggtctcgcc ctcgaagacg ttggcgccgt cgcggaagac ggtcacctgg
     2701 tcggtcaggt tgaagatctc gtcgagccgg tggctgacgt agacgatcgc gacgccgtcg
     2761 ttgcggaggc tgcgcaggac cttgtcgagg tgctcgatct cgctgtcggt gagcgaggcg
     2821 gtgggctcgt cgagcacgat catgcgcgcg tcggcggcga gcccgtgggc gatcacgacg
     2881 agccggcgct cggcgacgct gaggctggtc agcggcgccg ccgggttgat gtcggcctcg
     2941 agccggtcga gcgcctggcg ggctttcttg cgcatcgccc gggtgcggac gaggctgccg
     3001 gcgacggtcg ggtaaccgag cccgagcagc acgttctcgg ccacggtcag gttcggcacg
     3061 tccatcagtt cctggtgggc gaaggcgaag ccgagggcgc tggccgcgtg cgggttgggg
     3121 atcgaggtcg gctcgccgtt gaccaggatc tcgccctcgt ccgggtggac cgcgccggcc
     3181 agcaccttga tcaccgtgct cttgccggcg ccgttcttgc cgagcaggcc gtggatccgg
     3241 ccaccctcga ttgtgaggtc ggcgccgttg agggcgcgca cgccggggta ctgcttgacc
     3301 agcccccggg cctcgatctt cagcggtgcg gtgacggcgt tcatcacgcc cgctgaccca
     3361 ggcggctgac caggacggcg gcaatcagga tcccgccctg gaccaggttg attacgtagc
     3421 tttcggtgtt cagcatcacc aagccggtct ggatcacacc gaggaagagc acgccgacga
     3481 cggtgcccgg gatgttgaac tcacccggcc tgaacaccgc tgcaccgagg aaggcggccg
     3541 cgaacgcggg caacaggtag gtggcgccgc cgttcggcga gtaggagccg gagagcgaga
     3601 tcatcaggat gccgacgatc gccgcggcga ggccgacggc gatgaagccg atcacccggt
     3661 agggccggac ccggagcccg gagaggcggg ccgcctcttc gttgcc
//
LOCUS       DASRIG010000553         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1817715, whole genome shotgun sequence.
ACCESSION   DASRIG010000553 DASRIG010000000
VERSION     DASRIG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817715"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFB52_14755"
     CDS             <1..769
                     /locus_tag="VFB52_14755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZO07647.1"
                     /translation="VLTRRADLVVTNSQYVADQFKGLKARRPVRALYDPVDISGFDPN
                     ADPAPVRREFAIGRETVVLAVVAQLTPWKAQDDAIRALAALPTGGPDTALLLAGSAVF
                     SGEGTKFDNQAYVDGLHRLASELGVADRVHFLGERADVPAVLAATDIVLLPSWREAYG
                     RIAVEGLAMGVPVVATEVGGPAEIVRPGVDGLLLPPKSPDLWARELGPLVDSASQRAE
                     MGANGVERAQNFSVPTQMRELLAVYAELTGKSPGAAL"
     gene            783..2216
                     /locus_tag="VFB52_14760"
     CDS             783..2216
                     /locus_tag="VFB52_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZO07648.1"
                     /translation="MARTITRSGVASAFVAVLVALFCVALIKFSDQLAATAVLLLFLV
                     GLRLRSRSAGLVALWCFWLFTPMLRRIIELGGEAPAADPLAVMPIIATGLLAVMELQE
                     NRLNARARTILILAVGGILLGVPLGLTIDPAATTFAAMAYVAGVSAFVIGWGDEIRGE
                     RTTLERFLTIGLVPIALYGIAQYFLPFTPWDAQWVDRVSQAGLGSIISPQEEKIRVYG
                     TLNSPFTFAIVLSIGIIFALAARRKFGARMLVLLPLVLALALTYVRSAWVSLVVGLIV
                     YAACGSDRSATRTVVGVFVCLAAVVVLGGSNPTTAAFSERITSLGDLNSDTSAQDRLG
                     VSARLVPESVANPFGTGLGQAGLAANLSESKEEGVVDIDNGYLAILYQCGPLAFLLVI
                     CAFVYSVRAAIGALERASPDERPYRAAVVAILLMMLVAEASGDVMFGLPGVIFWYLAG
                     SSVAACRTEKAALPINSREPLASGAPA"
     gene            complement(2197..3117)
                     /locus_tag="VFB52_14765"
     CDS             complement(2197..3117)
                     /locus_tag="VFB52_14765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZO07649.1"
                     /translation="MLIPTWRRPKPLRRCLQALATQTRAPDQVVLVVRADDEETRGLL
                     AGLELPYPVQVVEPPRTGVVAALNAGFDAVEGDVVAIADDDTEPLPDWLAKIEAHFEA
                     DPQLGGLGGRDRIVGSDEQPPVPADQLSVGTVLWFGRVTGNHHLAAGPPREVDILKGA
                     NMSWRTAALGTHRINPALRGRAAEHNWEIELSLAIQADGWRLVYDPEVELLHHEAPRP
                     FGERERQMSAQERFDAVHNQTLALASHLRGARRLRALAYDFLFGTRQNPGPVLALETV
                     LRRTASPRETLGRLRVATRARFAALRQARR"
     gene            3284..>3795
                     /locus_tag="VFB52_14770"
     CDS             3284..>3795
                     /locus_tag="VFB52_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZO07650.1"
                     /translation="MSTERKSESSDLSLIAQAIRRRLKIVVICLIVGIAGAVVLTIQS
                     DKQYSATSSILLNPSSGFEPQRTVETNLELLGLPLLAVRTAEEVGGLSSSEVSSAVSV
                     SQQGESNIVQVEATTDDPDLAAEIANSYAEQYVLFQEGGEREKFAGRVRVVERAVPDD
                     TPVSPKPTRNV"
BASE COUNT          523 a         1359 c         1313 g          600 t
ORIGIN      
        1 cgtgctcacc cgccgcgccg acctcgtcgt caccaactcc cagtacgtcg ccgaccagtt
       61 caaggggctg aaggcgcgcc ggccggtgcg ggcgctgtat gacccggtcg acatctccgg
      121 cttcgatccc aacgcggatc cggccccggt gcgccgcgag ttcgcgatcg ggcgcgaaac
      181 cgtggtgctg gcggtggtcg cccagctgac cccctggaaa gcccaggacg acgcgatccg
      241 ggcgctcgcc gccctgccga ccggcggtcc cgacacggcc ctgttgctgg ccggctcggc
      301 cgtcttctcc ggcgagggca ccaagttcga caaccaggcc tacgtcgacg ggctgcaccg
      361 gctggccagc gagctcgggg tcgccgaccg cgtccacttc ctcggcgaac gcgccgacgt
      421 gccggcggtg ctcgccgcca ccgacatcgt cctgctgcct tcctggcgcg aggcctacgg
      481 ccggatcgcg gtcgaggggc tggcgatggg cgtgccggtg gtcgcgaccg aggtcggcgg
      541 accggcggag atcgtccgcc ccggcgtcga cggcctgctg ctgccgccga aatcccccga
      601 tctctgggcc agagagctcg gtcccctggt cgacagcgcc tcccagcgcg ccgagatggg
      661 cgccaacggc gtcgagcgcg cccagaactt ctccgtcccg acccagatgc gcgagctgct
      721 cgccgtctac gccgagctga ccggaaagag cccaggggcg gctctatagt gcgcccctcg
      781 tgatggcccg cacgatcacc cgctcgggcg tggcgagcgc cttcgtggcc gttttggtcg
      841 cgctcttctg tgtcgcgctg atcaaattct ccgaccagct ggcggcgacc gcggtgctgc
      901 tgctgttcct ggtcgggctg cggttgcgct cgcgcagcgc cggcctggtc gcgctctggt
      961 gcttctggct gttcacgccg atgctgcggc ggatcatcga actcggcggc gaagcgccgg
     1021 cggccgaccc gctggcggtg atgccgatca tcgccaccgg cttgctggcg gtgatggagc
     1081 tgcaggaaaa ccggctgaac gcgcgtgccc gcacgatcct gatcctcgcc gtcggcggca
     1141 tcctgctcgg ggtgccgctg ggcctgacga tcgacccggc ggcgacgacc ttcgcggcga
     1201 tggcctacgt cgcgggcgtc tccgccttcg tgatcggctg gggcgacgag atccgcggcg
     1261 agcgcacgac cctggaacgc ttcctgacga tcggcctggt cccgatcgcg ctctacggga
     1321 tcgcccagta cttcctcccg ttcacgccct gggacgcgca gtgggtcgac cgggtcagcc
     1381 aggccgggct cggcagcatc atctcgccgc aggaagaaaa gatccgtgtc tacgggaccc
     1441 tgaactcgcc cttcaccttc gcgatcgtgc tctcgatcgg gatcatcttc gcgctcgccg
     1501 cgcggcgcaa attcggcgcc cggatgctgg tcctgctgcc gctggtgctg gcgctggcgc
     1561 tcacctacgt gcgcagcgcc tgggtctcgc tggtcgtcgg gctgatcgtc tacgccgcct
     1621 gtggcagcga tcgcagcgcc acccgcaccg tggtcggcgt cttcgtctgc ctcgccgcgg
     1681 ttgtcgtgct ggggggcagc aacccgacca cggcggcgtt cagcgaacgg atcacctcgc
     1741 tcggcgacct caactccgac acctctgctc aggaccggct cggggtctcg gcccggctgg
     1801 tgcccgaatc ggtcgccaac ccgttcggca ccgggttagg tcaggccgga ctggccgcga
     1861 atctgagcga atcgaaagaa gagggcgtgg tcgacatcga caacggctac ctggcgatcc
     1921 tctaccagtg cgggccgctc gccttcctgc tggtcatctg cgcgttcgtc tacagcgtgc
     1981 gcgccgcgat cggggcgctc gagcgggcca gccccgacga gcggccctac cgcgccgcgg
     2041 tcgtggcgat cctgctgatg atgctggtcg ctgaggcctc gggcgacgtg atgttcggct
     2101 tacccggcgt gatcttctgg tacctggccg gatcctcggt cgctgcctgc cggaccgaaa
     2161 aagcggcgct gccgatcaac tctcgcgaac cgctggctag cggcgcgcct gcctgagcgc
     2221 ggcaaagcgg gccctggtcg cgacgcgcag gcggccgagc gtctcgcgcg gcgaggcggt
     2281 gcgtcgcagc accgtctcca gcgccagcac cggccccggg ttctggcggg ttccgaagag
     2341 gaagtcgtag gcgagcgcgc gcaggcggcg ggcgccgcgc aggtggctgg ccagggcgag
     2401 cgtctggttg tggaccgcgt cgaagcgttc ctgggccgac atctgccgct cgcgctcgcc
     2461 gaacggccgc ggcgcctcgt ggtggagcag ttcgacctcc gggtcgtaga cgaggcgcca
     2521 gccgtcggcc tggatcgcga ggctgagctc gatctcccag ttgtgctcgg cggcacgccc
     2581 gcgcagcgcc gggttgatcc ggtgcgtgcc gagcgccgcg gttcgccagg acatgttcgc
     2641 gcccttgagg atgtcgacct cgcgcggcgg cccggcggcg aggtgatggt tcccggtcac
     2701 gcgcccgaac cagagcaccg tgccgaccga gagctggtcc gcggggaccg gcggctgctc
     2761 gtcggagccg acgatccggt cgcgtccgcc cagcccgcca agttgcgggt cggcctcgaa
     2821 gtgagcctcg atcttcgcca gccagtcggg cagcggctcg gtgtcgtcgt cggcgatcgc
     2881 gaccacgtcg ccctcgaccg cgtcgaagcc ggcgttgagc gcggccacca cgccggtgcg
     2941 cggcggctcg accacctgca ccggataggg gagctcgagg ccggctaaaa ggccgcgcgt
     3001 ttcctcgtcg tcggcgcgga ccacgagcac gacctggtcg ggcgcgcggg tctgcgttgc
     3061 cagcgcctgc aggcagcgcc gcagcggctt gggacggcgc caggtcggga tcaacatcga
     3121 gattcgcatc ggggcgctgg aggatcgcac gcctggaggc agaagctccc accgatagga
     3181 gcagaatccc cgatcgtccg aatggccatt ccatcgggta tgggttggta gattcgccgc
     3241 tcggaatccc gcgcaggaac gcctgaggaa cgcccacgcc cccgtgtcaa ccgaacgcaa
     3301 atccgaatcg tcggacctat cgctgatcgc ccaggcgatc cggcgtcggc tcaagatcgt
     3361 cgtcatctgc ctgatcgtcg ggatcgctgg cgcggtcgtg ctgacgatcc agtccgacaa
     3421 gcagtactcg gcgacctcct cgatcctgct caatccctct tcgggcttcg aaccgcagcg
     3481 cacggtcgaa accaacctcg agctgctcgg cttgccgctg ctggcagtcc ggaccgccga
     3541 ggaagtcggc ggcctcagca gctccgaagt cagctccgcg gtcagcgtct cccagcaggg
     3601 cgaatcgaac atcgtccagg tcgaagcgac caccgacgac cccgacctcg ccgccgagat
     3661 cgccaactcc tacgccgagc agtacgtgct cttccaggaa ggcggcgaac gggaaaaatt
     3721 cgccggccgc gtacgcgtgg tcgagcgcgc ggtgcccgac gacacgccgg tctcgccgaa
     3781 gccgacccgc aacgt
//
LOCUS       DASRIG010000554         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2072149, whole genome shotgun sequence.
ACCESSION   DASRIG010000554 DASRIG010000000
VERSION     DASRIG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072149"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFB52_14775"
     CDS             complement(<1..604)
                     /locus_tag="VFB52_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZO07651.1"
                     /translation="MPGIRTNAAAEVLGVSPNTLRSWERRYGFPEPKRTEGNHRNYDL
                     VELQTLRDALAQTGNISSAIALTRQRQEAPASGGDLVVAFEGFDEAAADRAIEESLAV
                     RPLERTVEELLLPAIDQLAADPARAAELEFGSRWATGWLHGARRLAATASRPAGILLL
                     DSSRGMDAEEVHAQALDLSLRRAGFRVLMLSNELGDERLER"
     gene            complement(690..1430)
                     /locus_tag="VFB52_14780"
     CDS             complement(690..1430)
                     /locus_tag="VFB52_14780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZO07652.1"
                     /translation="MAAHAGNGNGSSGDSGGSPAGRLAREAAAQQAALLAKLIEEIGT
                     EDGAALAAAAQRLAAEYGEVTAAAVDAAYAHAAASAEPLPWSPPEELPSLYRPGHMRR
                     RLDQLIETNHRYGHPFGVAVFDASGPGTRNGDPGGGRETVLAIVGAALRDSIRIVDEA
                     FQLEEDAICVLAPNLDTVEGVRMAERLLGKLDELERAGGLRIALAAGVVACPDHGADA
                     DALLHKADAALWRARAVGQPLGVGALAS"
     gene            complement(1563..2165)
                     /gene="recR"
                     /locus_tag="VFB52_14785"
     CDS             complement(1563..2165)
                     /gene="recR"
                     /locus_tag="VFB52_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZO07653.1"
                     /translation="MSTEGPAPLARLVAELSRLPGIGQRTAQRLAFHLLRAPDEEAFA
                     LGDAIREVKEKVGLCEQCFNLAEGPLCRICADVNRDRTLICVVEEPADVIPLERTHEY
                     RGLYHVLGGALSPIDGVDAEDLKIAELIGRVERGGIEEVVLATNPTTTGEATALHIAE
                     RLRGQVNVTRLAAGLPVGADLEHADEVTLGRALAGRRSLD"
     gene            complement(2162..2509)
                     /locus_tag="VFB52_14790"
     CDS             complement(2162..2509)
                     /locus_tag="VFB52_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZO07654.1"
                     /translation="MARGGGPGGFDVNALMKQAQQMQNQMLEAQEKLKDETVEASAGG
                     GMVKVVVGGDMTLREISIDPEAIDPEEAEMLEEMVKAAVDAGLRAAQEMASSKMGGIT
                     GDMGGGLGGMLGA"
     gene            complement(2510..4174)
                     /gene="dnaX"
                     /locus_tag="VFB52_14795"
     CDS             complement(2510..4174)
                     /gene="dnaX"
                     /locus_tag="VFB52_14795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZO07655.1"
                     /translation="MSAESTSLYRRHRPGSFDEVVGQQHVVRTLRNAVEGDKVHHAYL
                     FVGSRGTGKTSMAKILARSLNCERGGPTVTPCGECESCVTIAAGTSIDVIEMDAASNR
                     SVDDVRDLRERVAYAPAGGHWKVYILDEAHMLTKEAWNAFLKTLEEPPPKTVFVLATT
                     ESHKVMPTIADRCQRFDFQRPSLEQISEVLTRVAATESITIDDGAVAMIARSAQGSFR
                     DALGTLDQLVAFGGEQIGLDSVLEMLGAADADLLFDAVDAVTANDPKAVLLGVEKMAR
                     SGRDPAQFARDLLAHLRFLLVTQTTGEVPTSFVVTATDSDRLRAQAQTVGPATLVRTI
                     DELADALTAVREGDDARMAVEIALLKAARPDLDPSIEGLLRRIEQLEARLAGGSPPPA
                     PAPPPEPVIEDPPAPEPEPPAPEPEPPAPEPPAPAAAGGGDLSLADLQHIWGTVLAKL
                     AETAPALAATFEGARPDGLEGDQLSIGFPPDRTFNKRKAESPERREVLVTAIHAITGK
                     MLRPTYDLLDEDAEEAEPAGAAKPDGVDEEELLRRLKSEFDAEEVS"
     gene            complement(4261..4842)
                     /locus_tag="VFB52_14800"
     CDS             complement(4261..4842)
                     /locus_tag="VFB52_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07656.1"
                     /translation="MKKLARSFTRHLKSNVVGYVALFIALGGVAYAGAKPLLDVKGSV
                     DSANLKAGAVKGSDIAAGAISEGDLSGELQKGLGLDVPAPANGTYVGTGTATASGGSD
                     QPVTVTSVWRNGAPVSARMTSPSPICTSASFTAIRPEGPISWFVYLDPRLSGITFDYL
                     AKNAVMAEFGLNTASDGQQCTFNNIVVSLQPTG"
BASE COUNT          675 a         1735 c         1825 g          798 t
ORIGIN      
        1 cgcgctcgag ccgctcgtcg ccgagctcgt tggaaagcat caacacgcgg aagccggcgc
       61 ggcgcagcga gaggtcgagc gcctgcgcgt ggacctcctc ggcgtccatg ccgcggctcg
      121 agtcgagcag caggatcccg gccgggcggg aagcggtggc ggcgagcctg cgggcgccgt
      181 gcagccagcc ggtcgcccag cgggagccga actcgagctc ggcggcacgg gcgggatcgg
      241 cggcaagctg gtcgatcgcc ggcagcagca gctcctcaac cgtgcgctcc agaggccgga
      301 cggcgaggct ctcctcgatc gcgcggtcgg cagcggcctc gtcgaagcct tcgaaggcga
      361 cgaccaggtc gccgccgctg gccggcgcct cctggcgctg gcgcgtcagc gcgatcgccg
      421 aagagatgtt gccggtctgg gccagcgcgt cgcgcagcgt ctgcagctcg accaggtcgt
      481 agttgcggtg gttgccctcg gtgcgcttcg gctcgggaaa gccgtagcgg cgctcccagc
      541 tgcgcaacgt gttcgggctg accccgagaa cttctgcagc ggcgttggtc cggatacctg
      601 gcatctgcgg ggattcttcc accggaattc caggtttaca tcagttatgg acagatttcc
      661 ggtttccgcc agcgatcttg cacaggcttt tagctggcga gggcgccgac gccgagcggc
      721 tggccgaccg cgcgggcgcg ccagagcgcc gcgtcggcct tgtgcaggag tgcgtcggcg
      781 tcggccccgt ggtcggggca ggcgacgacg ccggcggcga gcgcgatccg cagcccgccg
      841 gcccgctcga gctcgtcgag cttgccgagc aggcgctcgg ccatccgcac gccctcgacc
      901 gtgtcgaggt tgggggcgag cacgcagatc gcgtcctcct cgagctggaa cgcttcgtcg
      961 acgatccgga tgctgtcgcg cagggccgcg ccgacgatcg ccagcacggt ctcgcgccca
     1021 ccgccggggt cgccgttgcg cgtgccgggc ccggaggcgt cgaagacggc gaccccgaac
     1081 gggtgcccgt agcggtggtt ggtctcgatc agctggtcga ggcggcggcg catgtgaccg
     1141 ggccggtaga ggctcggcag ctcttccggc ggtgaccagg gcagcggctc ggcgctggcg
     1201 gcggcgtggg cgtaggcggc gtcgaccgcg gccgcggtca cctcgccgta ctcggcggcc
     1261 aggcgctgcg ccgcagcggc cagcgcggcg ccgtcctcgg tcccgatctc ctcgatcagc
     1321 ttcgccagca gtgccgcctg ctgagcggcc gcctcgcgcg ccagccggcc ggccggtgag
     1381 ccgccggagt cgccactgga accgttgccg ttgcccgcgt gcgcagccac gcagggaccc
     1441 tgcctgaagc ggcggacgcc agccagcgca aaagttcgct atttgcggga caacacccgt
     1501 ccaggacaga tgttcaatcg cattcgacta gttggagtcg cctccagcgg agacgcgccc
     1561 cgctagtcga ggctgcggcg cccggcgagc gcccggccga gcgtgacctc gtcggcgtgc
     1621 tcgaggtcgg cgccgaccgg gaggccggcg gcgagccggg tgacgttgac ctggccgcgc
     1681 agccgttcgg cgatgtgcag cgcggtcgcc tcgccggtcg tggtcgggtt ggtcgccagc
     1741 acgacctcct cgatcccgcc gcgctcgacc cggccgatca gctcggcgat cttcaggtcc
     1801 tcggcgtcga ccccgtcgat cggcgacagc gcgccgccga gcacgtggta gaggccgcgg
     1861 tattcgtggg tgcgctcgag cgggatcacg tcggccggtt cctcgaccac gcagatcagc
     1921 gtgcggtcgc ggttgacgtc ggcgcagatc cggcagagcg ggccctcggc gaggttgaag
     1981 cactgctcgc agagcccgac cttctccttc acttcgcgga tcgcgtcgcc gagcgcgaac
     2041 gcctcttcgt cgggcgcgcg cagcaggtgg aaggcgagcc gctgggcggt ccgctggccg
     2101 atccccggca gtcgcgagag ctcggcgacg aggcgcgcga gcggtgccgg cccctcggtg
     2161 ctcaagctcc gagcatccct ccgaggccgc cgcccatgtc gccggtgatc ccgcccattt
     2221 tcgagctggc catctcctgg gcggcgcgca gaccggcgtc gacggcagct ttcaccatct
     2281 cctccagcat ctcggcctcc tctggatcga tcgcttccgg gtcgatcgag atctcgcgca
     2341 gggtcatgtc gccaccgacg accaccttga ccatgccgcc gccggcgctc gcctcgacgg
     2401 tttcgtcttt cagtttctcc tgggcttcga gcatctggtt ctgcatctgc tgcgcctgtt
     2461 tcatcagcgc gttgacgtcg aacccgccag gtccacctcc gcgtgccacc taactgacct
     2521 cctcagcgtc gaactcgctc ttcagccgcc gcagcagctc ttcctcgtca actccatcag
     2581 gtttggcggc gccagcgggc tcggcctcct cggcgtcctc gtccaacagg tcgtaagtcg
     2641 gccgcagcat cttgcccgtg atcgcgtgga tcgccgtcac cagcacttcg cgccgctcgg
     2701 gcgattcggc cttgcgcttg ttgaaggtgc ggtcgggcgg gaacccgatc gagagctggt
     2761 cgccctcgag cccgtcgggg cgggcgccct cgaaggttgc ggcgagggcc ggggcggtct
     2821 cggccagctt cgccaggacg gtgccccaga tgtgttgcag atccgcgagg gatagatcgc
     2881 ctccgcccgc ggcagcagga gccggtggct cgggtgccgg gggctcaggc tcgggtgccg
     2941 ggggctcagg ctccggtgcc gggggatcct cgatcaccgg ctccggcggc ggagcgggcg
     3001 caggaggtgg actccctccc gccagccgag cctccagttg ctcgatgcgt cgcagcaagc
     3061 cctcgatcga aggatcgagg tcggggcgcg cggccttcaa gagggcgatc tcgacagcca
     3121 tgcgggcatc gtcgccctca cgcactgcgg tgagcgcgtc ggccaactcg tcgatcgtcc
     3181 gcaccagcgt ggccgggccg acggtctggg cctgagcgcg caggcgatcc gagtcggtgg
     3241 cggtgacgac gaaggaggtc gggacctcgc cggtggtctg ggtgacgagc aggaagcgca
     3301 ggtgggcgag caggtcgcgg gcgaactggg ccgggtcgcg gcccgagcgg gccatcttct
     3361 cgaccccgag caggaccgcc ttcgggtcgt tggcggtgac cgcgtcgacc gcgtcgaaca
     3421 gcaggtcggc gtcggcggcg cccagcatct ccaggaccga gtcgagcccg atctgctcgc
     3481 cgccgaaggc gaccaactga tcaagcgttc cgagcgcgtc gcggaagctg ccctgggccg
     3541 agcgggcgat catcgccacc gcgccgtcgt cgatcgtgat cgactcggtc gcggccaccc
     3601 gggtcagcac ctcggagatc tgctccagcg agggccgctg gaagtcgaac cgctggcagc
     3661 ggtcggcgat cgtcggcatc accttgtgcg actcggtggt cgcgagcacg aagacggttt
     3721 tcggcggggg ctcctcgagc gtcttcagga aggcgttcca ggcctctttg gtcagcatgt
     3781 gcgcctcgtc gaggatgtag accttccagt ggccgccggc gggcgcgtag gcgacccgct
     3841 cgcgcaggtc gcggacgtcg tcgaccgagc ggttcgaggc ggcgtccatc tcgatcacgt
     3901 cgatcgaggt cccggcggcg atcgtcacgc aggactcgca ttccccgcag ggggtcaccg
     3961 tcggcccgcc gcgctcgcag ttcagcgatc tagccaggat cttcgccatc gaggtcttgc
     4021 cggtcccgcg tgagccgacg aacaggtagg cgtggtggac cttgtccccc tcgaccgcgt
     4081 tgcgcagcgt gcgcacgacg tgctgctggc ccacgacttc gtcgaaggag cccgggcggt
     4141 ggcggcggta gagggaggtg gactcggctg acattcgctc tgcggccagt gtatccacgc
     4201 ccctccgcct ccccggccgg ggcgccgccc tcgcctacga aggcgagagc ggcagcctgc
     4261 tcaccccgtt ggctgcaggc tcacgacgat gttgttgaac gtgcactgct gcccgtcgga
     4321 ggcggtgttg agaccgaact ccgccatcac ggcgttcttg gcgaggtagt cgaaggtgat
     4381 tccgctgagg cgcgggtcca ggtagacgaa ccaggagatc ggaccctccg gcctgatcgc
     4441 ggtgaagctg gcggacgtgc agatcgggct gggcgaggtc atcctggcac tgaccggtgc
     4501 tccgtttcgc cagaccgaag tgacggtcac gggctggtcg ctgccgccgg aggcggtggc
     4561 ggtaccggtg ccgacgtaag tgccgttcgc gggcgccggg acatcgaggc cgaggccttt
     4621 ctgcaactcg cccgagaggt cgccctcgga gatcgcgccg gcggcgatgt ccgagccttt
     4681 caccgccccg gccttgaggt tggccgagtc gacagagcct ttgacgtcga gcagaggctt
     4741 ggcgccggcg taggcgacac cgccgagcgc gatgaacagg gcgacgtaac cgacgacgtt
     4801 ggatttgagg tgacgggtga acgaccgagc aagttttttc atgcgcgcac gatacctctg
     4861 ggcggcgccc ccgatagcgt gcgcgcgtgc agacgaggct ctccagctgg ctcgtgccgg
     4921 ccgcatgggt cgccggggtg agcgcggtcc tgctggcgct cttcccggtc ggcttcccca
     4981 actacgacac gatctacgcg ctcctctggg gacgcgaact ggcgcacggg ctg
//
LOCUS       DASRIG010000555         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2331822, whole genome shotgun sequence.
ACCESSION   DASRIG010000555 DASRIG010000000
VERSION     DASRIG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331822"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFB52_14805"
     CDS             <1..1115
                     /locus_tag="VFB52_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZO07657.1"
                     /translation="AAGEAKRYRANDFSISRQRSWGTPIPIIHCESCGVVPVPVEDLP
                     VELPRDLVPSGEGNPLAERPDFVDVACPKCGEPAKRETDTLDCHFDALFLWVPAAVPP
                     EARAEQMFTHEELRDWLPAERLVAGGDSGGFVFDQRTVTKALRDHGEFAFMENGEPFA
                     GCLFHEMVIADGRKMSKHLGNVVDPDELVAEYGADTVRLAVLYAAGPAKTLNWSDGAV
                     RFANRFLRNLWTYSHDRFAQLPDLAHDPEAAADTEHMRDRLRKWCDNGLERITADTAE
                     LQMHKSIRNITRLFERIQDFEKRVVKRRGQLDRADAEAQIAALVLLAQVLAPFAPHTA
                     EEILLASGRPDGADLVGSWPERAEIPVGEDSAAAAP"
     gene            complement(1370..1633)
                     /locus_tag="VFB52_14810"
     CDS             complement(1370..1633)
                     /locus_tag="VFB52_14810"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZO07658.1"
                     /translation="MTTRERFAANLRRARTAAGISQEELGDRCDLHRTEISLLERAGR
                     EPRLGTIVKLAGALDTTPGELCTGIAWNVASHRFEVKQPPRSS"
     gene            1831..>3029
                     /gene="thrC"
                     /locus_tag="VFB52_14815"
     CDS             1831..>3029
                     /gene="thrC"
                     /locus_tag="VFB52_14815"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZO07659.1"
                     /translation="MSDAAQHLGGPAGQTSSCATELVCPSCGTTAELQDTAFRCPDCD
                     TAFDIAYDYELAKSRVEARPLGERPWNVWRFEELLPITSRYAADRVAQFAGQTPLIRA
                     ERLGAELGLRNLYVKDDSTNRPSLSYKDRVVGMAVARALELGASEIGCVSTGNVGTAT
                     ASLAAAAGLKPYVFYPANLERAKARGVRALGAQVCQVDGNYDEANRACRELTENTGIP
                     FANISLRPFYGEGAKTLAYELVETLGWRSPDHVVMPAAGGTLSSRVHKGLLEFETIGL
                     AETAHTKLHIGQPTGCSPIAQTIVTGAEEITPVQPETLAQSLAIGNPGDGPLVVEAVR
                     SRGGSAADVPDGEIITAMELLASTEGVLTEPAGGTTVAATMKLAQRGVIGPDDVTVIV
                     ISGNGLKT"
BASE COUNT          486 a         1062 c         1031 g          450 t
ORIGIN      
        1 ccgccgccgg tgaggcgaag cgctaccgcg ccaacgactt ctcgatctcc cgccagcgct
       61 cctggggcac gccgatcccg atcatccact gcgagagctg cggcgtcgtc ccggtcccgg
      121 tcgaggacct gccggtggag ctgccgcgcg acttggtccc gagcggcgag ggcaacccgc
      181 tggccgagcg ccccgacttc gtcgacgtcg cctgccccaa atgcggcgag ccggccaagc
      241 gcgagaccga cacgctcgac tgccacttcg atgcgctctt cctctgggtg ccggcggcgg
      301 tgccgccgga ggcgcgggcc gagcagatgt tcacgcacga ggagctgcgg gactggctgc
      361 cggccgagcg gctggttgcc ggtggcgact ccggcggctt cgtcttcgac cagcgcacgg
      421 tgaccaaggc gctgcgcgac cacggcgagt tcgccttcat ggagaacggc gagccgttcg
      481 ccggctgcct cttccacgag atggtgatcg ccgacgggcg caagatgagc aagcacctcg
      541 gcaacgtcgt cgaccccgac gagctggtcg ccgagtacgg cgccgacacc gtccgcctcg
      601 ccgtcctcta cgcggccggc ccggcgaaga ccctgaactg gtccgacggc gccgtgcgct
      661 tcgccaaccg cttcctgcgc aacctctgga cctactccca cgaccgcttc gcgcagctgc
      721 cggacctcgc ccacgacccc gaggccgccg cggacaccga gcacatgcgc gaccgcctgc
      781 gcaaatggtg cgacaacggg ctcgagcgga tcaccgccga caccgccgag ctgcagatgc
      841 acaagtcgat ccgcaacatc acccggctgt tcgagcggat ccaggacttc gagaagcggg
      901 tggtcaagcg gcgcggccag ctcgaccgcg ccgacgccga ggcgcagatc gcggccctcg
      961 tcctgctcgc tcaggtgctg gcgccgttcg cgccccacac ggccgaggag atcctgctcg
     1021 cctcgggtcg tccggacggg gcggacctgg tcggatcctg gcccgagcgg gccgagattc
     1081 cggtcggcga ggacagcgcc gcggccgcgc cttgaaaatt tgctccattg gacaaaaggg
     1141 ccttgattgt tacacgggcc tgtgttatga attcccctgc cgggcaatag aaccggtgag
     1201 gtgagcgggg caagcggact gggaaaccgc cttggggtgg caaaggagga ccggtcctta
     1261 gttccggggt cagaaaaaac gtcatgacgg gcctcggacg cgccggccta gagtcggcgc
     1321 ccccgcctca cttcaccgtt ctcggtcgtt cgcgaggcag ttgacgggtt cagctgctgc
     1381 ggggcggctg cttgacctcg aaccggtgcg aggcgacgtt ccaggcgata ccggtgcaga
     1441 gctcgcctgg agtggtgtcg agtgcgccgg ccagcttcac gatcgtgccg aggcgcggct
     1501 cgcgcccggc ccgctcgagc agcgagatct cggtccggtg taggtcgcag cgatcgccca
     1561 gctcttcctg ggagatgccc gctgcggtgc gggcgcgacg gaggttggcg gcgaaccgct
     1621 cacgagtggt caagtggcca attcttggag actgaagaca aggactctac agacgaaatg
     1681 tttcgcccca ttcgcgctgc ctggcgcttc cgctaccttc cagtgcagga gctatctgcg
     1741 atatctggtt atttacgcag aaagcattga gcactgacat cctatgtgtt acaaccgtgc
     1801 cttaattcgt accgtcatca aggaggagca atgagcgacg cagcgcagca tctggggggg
     1861 cccgccgggc agaccagttc ctgcgcgacc gaactcgtct gcccctcgtg cgggacgact
     1921 gcggaacttc aggacaccgc cttccggtgt ccggactgcg acaccgcctt cgacatcgcc
     1981 tacgactacg agctggccaa gagccgggtc gaggcgcgcc cgctcggcga gcgcccctgg
     2041 aacgtctggc gcttcgagga gctgctcccg atcacaagcc gctacgcggc cgaccgggtc
     2101 gcccagttcg ccggccagac gccgctgatc cgcgccgagc ggctcggcgc cgagctcggc
     2161 ttgcgcaacc tctacgtcaa ggacgactcg accaaccggc cctcgctctc ctacaaggac
     2221 cgcgtcgtcg gcatggccgt cgcccgggcg ctggagctgg gcgcgtcgga gatcggctgc
     2281 gtctcgaccg gcaacgtcgg caccgcgacc gcctcgctgg ccgcggcggc cgggctcaag
     2341 ccctacgtct tctacccggc caacctggag cgggcgaagg cgcgcggcgt gcgcgcgctc
     2401 ggcgcccagg tctgtcaggt cgacggtaac tacgacgaag ccaaccgcgc ttgtcgtgag
     2461 ctgaccgaaa acaccggaat cccgttcgcc aacatcagcc tgcggccgtt ctacggcgag
     2521 ggcgcgaaga cgctcgccta cgagctggtc gagacgctcg gctggcgctc gcccgaccac
     2581 gtggtgatgc cggcggcggg cggaaccctg tcctcgcgtg tgcacaaggg gctgctcgag
     2641 ttcgagacga tcgggctcgc cgagaccgcg cacaccaagc tccacatcgg tcagccgacc
     2701 ggctgttcgc cgatcgcgca gacgatcgtc accggcgccg aggagatcac tccggtgcag
     2761 ccggagacgc tggcgcagtc gctggcgatc gggaaccccg gtgacggccc cctggtggtc
     2821 gaggcggtgc gcagccgcgg cggctccgcc gccgacgtgc ccgacggcga gatcatcacg
     2881 gcgatggaac tgctggcctc gaccgagggc gtgctcaccg agccggccgg cgggaccacg
     2941 gtggcggcga cgatgaagct cgcccagcgc ggcgtgatcg gccccgacga cgtgaccgtg
     3001 atcgtgatca gcggcaacgg cctgaagac
//
LOCUS       DASRIG010000556         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2335692, whole genome shotgun sequence.
ACCESSION   DASRIG010000556 DASRIG010000000
VERSION     DASRIG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335692"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFB52_14820"
     CDS             <1..383
                     /locus_tag="VFB52_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZO07660.1"
                     /translation="AIAPMLEQRSGAIVNVASITAVTALPGLGAYTASKHAVIGLTRV
                     GAIEGAGGGVRVNAVLPGPTATRMIGTEDFSAQVPLGRAADPSEPAAVICFLASDAAS
                     FVTGESILVDGGMAYAGRGLEAAE"
     gene            complement(350..703)
                     /locus_tag="VFB52_14825"
     CDS             complement(350..703)
                     /locus_tag="VFB52_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZO07661.1"
                     /translation="MEPKSLATVLPVPNLEQAVAFWRGALGVEPSFVDGDRWAQFDFG
                     GRRIALAGSDRTSDLAGVMLKVDDLEAAASELADLGIELGPAEEGEHERRRTGTAPGG
                     WSFVIYSAASRPRPA"
     gene            complement(703..1950)
                     /locus_tag="VFB52_14830"
     CDS             complement(703..1950)
                     /locus_tag="VFB52_14830"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZO07662.1"
                     /translation="MTPTERRDRRGELAAAALDVFNEKGYGAASMRDIAARVGLLKGS
                     VYHYTDSKEELLARIFEQATDRTFAILEGVGRSEQPAIERLAAFVEGWSRWYLENLKV
                     IRVYLQEWKHLSGERLQRAIATRREAYERVVRLLEEAKEEGAAPTDLDCRYASLLILS
                     AINGLPGWYRPGQNEPAELVAETYSNLLVGMVTHSGPREPLAAAARTRLDGMIAAVPA
                     RDGGAEGRGETVLRAAIEVFHERGYAGASMREVAERSQMNKGSLYHYIDSKEALLAEI
                     FAASTERTFALLAEIEAMPGPAITRLGAFVECWSRWYLLNVKVITVYIDDWKHLSGER
                     LQRAIAIRREAYERVARLLEEIKAEGDAPADLDCRYAALLILSAINGLPGWYRPDQNE
                     SAERVAANYSDLLVGMVSNGKET"
     gene            complement(1955..2470)
                     /locus_tag="VFB52_14835"
     CDS             complement(1955..2470)
                     /locus_tag="VFB52_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZO07663.1"
                     /translation="MNPLLGSGGEADERCALVRRLARLEDQRAIERLKYAYTAALDGG
                     YDLDRICSLFTDDGRWVATGFGDHCGHAAIRGFFAGLKEEIAYAHHYASGPSIEIAAN
                     GEEASGEWALLCVSGRRHRDDPSVHLPVVELGRYHDRLVKREGRWLFAELRVEVTLSK
                     QVADLLSRPAV"
BASE COUNT          358 a          923 c          872 g          391 t
ORIGIN      
        1 acgcgatcgc cccaatgctc gagcagcgct cgggggcgat cgtcaacgtc gcctcgatca
       61 ccgcggtcac cgcgctgccc gggcttggcg cctacacggc gagcaagcac gcggtgatcg
      121 ggctgacccg ggtcggcgcc atcgaggggg ccggcggcgg cgtccgcgtc aacgccgtcc
      181 ttcccggccc gactgcgacc cgcatgatcg gcaccgagga tttctcggcc caggtcccgc
      241 tgggccgtgc cgccgacccc tccgagccgg ccgcggtgat ctgcttcctc gcctccgacg
      301 ccgccagctt cgtcaccggc gagtcgatcc tggtcgacgg cggcatggcc tacgccggcc
      361 gcggcctcga agcggctgag tagatgacga acgaccagcc gccgggcgcc gtgccggtgc
      421 ggcggcgctc gtgctcgccc tcctctgcgg gtccaagctc gatgccgagg tcggcgagct
      481 cgctggcggc ggcctcgagg tcgtcgacct tgagcatgac gccagccagg tcggaggtgc
      541 ggtcgctgcc ggcgagcgcg atccgacggc cgccgaaatc gaactgggcc cagcggtcgc
      601 cgtcgacgaa gctgggctcg acccccagcg cgccgcgcca gaaggcgacc gcctgctcga
      661 ggttgggaac gggcaacacg gtcgcgaggc tcttcggttc catcacgtct cctttccgtt
      721 ggacaccatg ccgacaagca ggtcggagta gttcgccgcc acccgctcgg ccgactcgtt
      781 ctggtcgggc cggtaccagc caggcagccc gttgatcgcg gagaggatca gcagcgcggc
      841 gtagcggcag tcgaggtcgg cgggggcatc cccctcggcc ttgatctcct cgagcagccg
      901 tgcgacccgc tcgtaggctt cgcgccggat cgcgatcgcc cgctgcaggc gctcgccgct
      961 gaggtgtttc cagtcatcga tgtagacggt gatcaccttc acgttgagca gataccaccg
     1021 cgaccagcac tcgacgaagg cgccaagccg ggtgatcgcc ggccccggca tcgcttcgat
     1081 ctcggccagc agggcgaagg tgcgctcggt cgaggcggcg aagatctcgg ccagcagcgc
     1141 ctccttggag tcgatgtagt ggtagaggct gcccttgttc atctgcgagc gctcggcgac
     1201 ctcacgcatc gaagcgccgg cgtagccacg ctcgtggaag acctcgatcg ccgcccgcag
     1261 caccgtctct ccccgccctt cggcgccgcc gtcccgggca gggaccgcag cgatcatccc
     1321 gtcgagccgg gtccgggccg cggccgcaag cggctcgcgc gggcccgagt gggtgaccat
     1381 cccgaccagc aggttcgagt aggtctcggc gaccagctcg gccggctcgt tctgtcccgg
     1441 ccggtaccag cccggcaggc cgttgatcgc cgagaggatc agcagcgagg cgtagcggca
     1501 gtcgaggtcg gtcggcgcgg cgccctcctc ctttgcttcc tcgagcaggc gcacgacccg
     1561 ttcgtaggct tcgcgccggg tcgcgatcgc gcgctgcagg cgctcgccgc tgaggtgttt
     1621 ccactcctgc aggtaaaccc tgatcacctt caggttctcc aggtaccaac gcgaccagcc
     1681 ctcgacaaag gcggcgagcc gctcgatcgc cggctgctcg gagcggccga cgccctcgag
     1741 gatcgcgaag gtgcggtcgg tcgcctgctc gaagatccgc gccagcagct cttccttgga
     1801 gtccgtgtag tggtaaacgc tgcccttcag cagcccgacc cgggcggcga tgtcgcgcat
     1861 cgaggcggcg ccgtagccct tctcgttgaa gacgtcgagc gccgccgcgg ccagctcccc
     1921 gcgccggtca cgtcgctcag tcggagtcat cggctcagac ggccggccgg ctcaggaggt
     1981 cagcgacctg tttgctcagc gtgacctcga cccgcagctc ggcgaacagc cagcggccct
     2041 cgcgcttgac cagccggtcg tggtagcgcc ccagttcgac caccggcaga tggaccgagg
     2101 ggtcgtcgcg gtggcggcgg ccggagacgc agagcagcgc ccactcgccg ctcgcctcct
     2161 ccccgttcgc ggcgatctcg atgctcggtc cggaggcgta gtggtgggcg taggcgattt
     2221 cctccttgag cccagcaaag aacccgcgga tcgccgcgtg accgcagtgg tcgccgaacc
     2281 cggtggcgac ccaacggccg tcgtcggtga acagcgagca gatccgatcg aggtcatagc
     2341 cgccgtcgag cgccgccgtg taggcgtact tgaggcgctc gatcgcccgc tggtcctcga
     2401 ggcgggcgag ccggcggacg agcgcgcagc gctcgtccgc ctcccccccg ctgcctagca
     2461 gtgggttcac gtcagtcgcg gaaattcggc cagacgtcgt ccgcgaacat cttcagctgc
     2521 cgcttctgct cgtccagcgg cagg
//
LOCUS       DASRIG010000557         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2597274, whole genome shotgun sequence.
ACCESSION   DASRIG010000557 DASRIG010000000
VERSION     DASRIG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597274"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFB52_14840"
     CDS             complement(<1..497)
                     /locus_tag="VFB52_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07664.1"
                     /translation="MAPAIRTTIAGLTALAVLALAGGVASADERAHTSIGFQPVPLSV
                     ATDAGGHVYLSNPNFSNTIEQYSADGALLAHLGNFPRSGSPLQPRAIASDAAANLYVA
                     DASSDLLAVLGPDGSALRSWSADGRDLAIGREGSVYVLGASEVSRFSPEGTLLSKWGS
                     SGEGDG"
     gene            528..1313
                     /locus_tag="VFB52_14845"
     CDS             528..1313
                     /locus_tag="VFB52_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07665.1"
                     /translation="MLGGRRIVLLVALGACLLPVTSAGGDISGPLPNDRYWLGTYYAG
                     LELNGPVEPDDRTYSYGDCELPEGEGGCSVPVQIQNWTSCTRNPIGLDSLPIKVFAVR
                     GGGLAAAYESTAVDIGTGTETVTVFTNEVEMVPAVLRDVHRRSQAGPEPLAPPVYPLP
                     VLRELVRVTALAGRLGVKEIARETGLRLNEVKLRIRIAKLLGPEALAGVKPPTMSIET
                     VERLRQLSFKASFNFAGTAREAGLTKAQLRKKVNLVRGLAGGC"
     gene            complement(1335..2255)
                     /locus_tag="VFB52_14850"
     CDS             complement(1335..2255)
                     /locus_tag="VFB52_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07666.1"
                     /translation="MPRARTLLTAIVTTALLSVLPAGTASAASPEGGTIVRVKFEPRA
                     SDGFEARLETSEKKVVRLELERGGSSVIYEVPGEVSEAGLKVRFGKLGLIDVAFTPTK
                     TLSTTAPAGGCTGAPRTLREGVFTGTIAFTGERGYVRLEGPEASGTMSVISQWDCPGS
                     EVALPYGNTSLLSSLRPHKAGSERESASLHLFSFRRPVAFAAGVHHRHSGGRSIFVGY
                     KGELREGMEIIRSTAASGAASRFDYDHKAGTASLRPPRPLSGQATFKRRPDAKPLWRS
                     TIKVPLLGADPIDTSGPGFRVGLYPEYHFD"
     gene            complement(2279..3313)
                     /locus_tag="VFB52_14855"
     CDS             complement(2279..3313)
                     /locus_tag="VFB52_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZO07667.1"
                     /translation="MSPVASETIERDLERLRPALTGYCYRMLGSGFEAEDATQETMVR
                     AWRRADSLAEPAALKSWLFRIATNVCLDQIEERKRRARPVDLGEAGTAETPVGAPLPE
                     TTWVLPIADSQVADPDADPAAQAAERESLRLAFVAALQHLPARQRVVLVLREVLRWSA
                     QEVADLLETSVASVNSALQRARATLDELALEDAEAPARPSDEDERKLLEQYLDAFAEY
                     DIDRIVALLRYDVVFDMPPLPLWVRGPEQVATFMLGQGAACAGSKLIATEANGFPAFA
                     AYKPDPESGAWLPWSITILELDAPDGSSPAVAGIHNFLAPFMPGLFESFGLPERRTDA
                     DPVVAWSGLS"
BASE COUNT          475 a         1144 c         1245 g          495 t
ORIGIN      
        1 ccgtcgcctt cgccgctcga tccccatttg gagagcaggg tgccttcggg ggagaagcgc
       61 gagacctcgc tcgccccgag cacgtagacg ctgccctcgc ggccgatcgc gaggtcgcgg
      121 ccgtccgcgc tccacgagcg cagcgcgctg ccgtccggcc cgagcacggc gagcaggtcg
      181 ctgctggcgt cggcgacgta gaggttggcg gcggcgtcgc tggcgatcgc gcgcggctgc
      241 agggggctgc cggacctggg gaagttgccc aggtgggcaa gcagcgcgcc gtcggccgag
      301 tactgctcga tcgtgttgct gaagttcggg ttcgagaggt agacgtggcc gccggcgtcg
      361 gtggcgaccg agagcgggac cggctggaag ccgatcgagg tgtgggctcg ctcgtcagcg
      421 ctcgccaccc cacccgcgag ggcgagaacg gcgagagccg tgagcccggc gatcgtggtc
      481 cgaatagcgg gagccacgga agcagatggt agacgtaaca ttcccgaatg ctcgggggcc
      541 gccggatcgt tctgctcgtt gcgctgggcg cctgcttgtt gcccgtcacc tcggcgggcg
      601 gggacatcag cggaccgctc ccgaacgacc gctactggct cgggacctac tacgccgggc
      661 tcgaactgaa cgggccggtc gaaccggacg atcggaccta cagctacggc gactgcgaac
      721 tgcccgaggg cgagggcggc tgctcggtcc cggtccagat ccagaactgg accagttgca
      781 cgcgcaaccc gatcggtctc gactcccttc cgatcaaggt cttcgcggtg cgcggcggag
      841 ggctggcggc cgcctacgaa tcgaccgccg tcgacatcgg gaccggcacc gagacggtga
      901 ccgtgttcac caacgaagtc gagatggtcc ccgccgtcct gcgcgacgtt catcgccgct
      961 cgcaggccgg tccggagccg ctggcgccgc cggtctaccc gctgccggtg ctgcgcgagc
     1021 tggtccgcgt gaccgcgctc gccggccggc tcggggtcaa ggagatcgcc cgggagaccg
     1081 gcttgcgcct caacgaagtg aagctgcgga tacggatcgc caagctgctc gggccggagg
     1141 cgttggccgg ggtgaagccg ccaacgatgt cgatcgagac ggtcgagcgg ctgcgccagc
     1201 tctccttcaa ggcttccttc aacttcgccg gcaccgcccg cgaggcgggg ctgacgaaag
     1261 cgcagctgcg caagaaggtc aacctggtcc gcggcctcgc cggcggctgc tgagcacccg
     1321 ccggccggag ccggctagtc gaagtggtat tcggggtaga ggccgacgcg gaagccgggg
     1381 ccgctcgtgt cgatcggatc ggctcccagc agcggcacct tgatcgtgct ccgccagagg
     1441 ggcttggcgt ccgggcgacg cttgaaggtc gcctgcccgc tcagcggtcg cggcggtcgc
     1501 aggctcgcgg tccccgcctt gtggtcgtag tcgaaacggg aggcggcgcc cgaggcagcg
     1561 gtcgaccgga tgatttccat gccttcgcgg agctcgccct tgtagccgac gaagatgctg
     1621 cgcccgccgc tgtggcgatg gtggacaccg gccgcgaagg cgaccggtcg ccgaaagctg
     1681 aagaggtgca gagaggccga ttcgcgttcg ctaccggcct tgtgtggccg taggctcgag
     1741 agcagcgagg tgttcccgta gggcagcgcg acttcgctgc cggggcaatc ccactgcgag
     1801 atcacgctca tcgtcccgct cgcctccggc ccttccagtc gcacgtagcc ccgctccccg
     1861 gtgaaggcga tcgtgcccgt gaagacgcct tcgcgcaggg tccgcggcgc gcccgtgcaa
     1921 ccccccgccg gcgccgtcgt gctcagcgtc ttggtcgggg tgaaggcgac gtcgatcagc
     1981 cccaacttcc cgaaccggac cttcagcccc gcttcgctga cttcgcccgg cacctcgtag
     2041 atcacgctgc tgccgccccg ctcgagttcg agcctgacca ctttcttttc cgaggtttcg
     2101 agccgcgctt cgaagccgtc gctggcccgc ggctcgaatt tgacccggac gatcgtgccc
     2161 ccttccgggc tcgcggcgga ggcggtgccg gccggaagga ccgacagcag agcggtggtc
     2221 acgatcgccg tgagcagagt tcgcgcgcgt ggcattccgc tcagaatacg ttccagcgct
     2281 aggaaagtcc cgaccaggcg accaccgggt cggcgtcggt gcgccgctcc ggcaggccga
     2341 aggactcgaa cagcccgggc atgaacgggg cgaggaagtt gtggatgccg gcaaccgccg
     2401 gggagctgcc gtccggagcg tcgagttcga ggatggtgat cgaccagggc agccaggcgc
     2461 cggactcggg gtccggtttg taggcggcga aggcggggaa gccgttggcc tcggtggcga
     2521 tcagcttcga gccggcgcag gcggcgccct ggccgagcat gaaggtcgcg acctgctcgg
     2581 ggccgcgcac ccagagcggc agcggcggca tgtcgaagac gacgtcgtag cgcagcaggg
     2641 cgacgatccg gtcgatgtcg tactcggcga aggcgtcgag gtactgctcg agcagcttgc
     2701 gctcgtcctc gtcgctgggc cgggccggcg cctcggcgtc ctcgagcgcg agctcgtcga
     2761 gggtggcgcg ggcccgctgc agggcgctgt tgaccgaggc gaccgaggtc tccagcaggt
     2821 cggcgacttc ctgggcgctc cagcgcagca cctcgcgcag gacgaggacg acgcgctggc
     2881 gcgccggcag gtgctggagc gcggcgacga aggcgagccg cagcgactcg cgctcggccg
     2941 cctgcgcggc cgggtcggcg tcggggtcgg cgacctggct gtcggcgatt ggcagcaccc
     3001 aggtggtctc cggcagcggc gccccgaccg gggtctcggc cgtgcccgcc tcgccgaggt
     3061 cgacggggcg ggcgcggcgc ttgcgctcct cgatctggtc gaggcagacg ttggtcgcga
     3121 tccggaacag ccacgacttc agcgccgccg gctcggccag gctgtccgcc cggcgccagg
     3181 cgcggaccat cgtctcctgg gtcgcgtctt cggcctcgaa cccggagccg agcatccggt
     3241 agcagtagcc ggtcagggcc ggtcgcaggc gctcgaggtc gcgctcgatc gtctcgctcg
     3301 ccacggggga catcgcgagc atgctagccc tcggccggct cgtagatcag cagggcgac
//
LOCUS       DASRIG010000558         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2338128, whole genome shotgun sequence.
ACCESSION   DASRIG010000558 DASRIG010000000
VERSION     DASRIG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338128"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1125
                     /locus_tag="VFB52_14860"
                     /pseudo
     CDS             8..1125
                     /locus_tag="VFB52_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            1122..2561
                     /gene="gatB"
                     /locus_tag="VFB52_14865"
     CDS             1122..2561
                     /gene="gatB"
                     /locus_tag="VFB52_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZO07668.1"
                     /translation="MSDLEAVIGLEIHVQLATETKMFCGCELSFGDEPNTHTCPICLA
                     HPGVLPVANEQAILYALQIGLALECEIAPRSIFHRKNYFYPDSPKAYQISQYDIPICG
                     AGRLGDVRIHRAHLEEDAAKTIHLGESGRIHGSAHSLVDFNRGGTPLVEIVTEPDLRD
                     AAEAAQWARLLRETVRQLGVSDVNMEEGSLRVDANVSVRPVGSEELGTKTELKNMNSF
                     RFLQRGIEAELERQKALIAAGGEVEQETLHFDPRSGELTSLRSKEEAHDYRYFPEPYL
                     VPIVPTEEMLREAREALPELPAARIARYEGDGLSTDVATVLATDPETAGYYEQVAAAA
                     SGVEARVVGNWVTGELVAALRAAEAEGGATASKVTPDALAALIGLVAEKKISHGSGKT
                     VLAALVEEGGDPAAIVEREGLAQMAADDGELEAIVAAAIEANPDAAAKIREGNNKAIG
                     AIVGHVMRETKGRADGGEVNRLIAQQLNS"
     gene            2625..3896
                     /locus_tag="VFB52_14870"
     CDS             2625..3896
                     /locus_tag="VFB52_14870"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZO07669.1"
                     /translation="MAWNVVIAGGGFGGAAAARELERVMPKQSARLVMANEQNFALYT
                     PFLPEAAAGTLEPRHVTTPLRDILKRTYLRLGSVVGHDPEAKTVQLAPKHGPLETLPY
                     DQLLLSLGSVSRVLPVPGLAEHAVGFKSLADAIYLRNHVIETLEEANATEDPARRDEL
                     LTYVFVGGGYAGLEALAELQDFAADAMDRYPRARLHGMRWVLVEAADRVLPEIDLELA
                     EYALRELRGRGIDIRMETTLEEVGADFARLSSGETLATQTVVWTAGVAPQPILKQLNL
                     PLDERGRVPVDSHLRVEGLDSVWAIGDCAAAPDPRGGLCPPTAQIAVRQGPVAARNIA
                     AELGIGSARPFEYRSEASFVNLGRYKAVGRIGERTFRGFPAWWLARSYHMSQIPGTAR
                     KARAVLDWTASLPFKRDISEVGSIGHPKPLV"
     gene            complement(3893..4450)
                     /locus_tag="VFB52_14875"
     CDS             complement(3893..4450)
                     /locus_tag="VFB52_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZO07670.1"
                     /translation="MRRAALAAACVALAAVLLLPAGGGAATPCVAWKKHHKRVVKWVK
                     KDGKRKKVVRVKRWRTCAKRAAPAPVTPPVVVPPPTEPQPEPEDPTIPRLGVKAVEWS
                     YTLSRPEVPAGEVVVELNNMGEDPHNLNLQLEDASGPEEKITTVGPGVQGKAKFNLPA
                     GEYRLWCDLDGHEAAGMKATLTVTP"
     gene            complement(4447..>5182)
                     /locus_tag="VFB52_14880"
     CDS             complement(4447..>5182)
                     /locus_tag="VFB52_14880"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZO07671.1"
                     /translation="PMIATADKPVAAIDSVDGYDLWSSVSDPVRAEMYKSFAKFGSLP
                     ADSKGLSQVRRATAQTDKLREDLGGVGDFSSPVAYPDTWFAHKLAGLAAYIDFGLPMR
                     VVTIRAAGGYDTHAGQAEDLDRNLGETIDGVLAFQRDLEARGLDDRVMIEMWSEFGRR
                     PEENGSAGTDHGAAGCAFVIGSKAKGEMVGEFPGLAKLDNYDNLRVTSDFRGMYCALL
                     EQWLGHDAGPIIPGADGFIQPTLVKA"
BASE COUNT          755 a         1884 c         1789 g          754 t
ORIGIN      
        1 gttcgcgatg ggctcctcga acgagaactc ggcctacggg ccagtgctca acccgtggga
       61 ccgcggccgc gttcccggtg gctcctcggg cggctcggcg gcggcggttg ccggcggcct
      121 cgcgccggcg gcgctcggta ccgacaccgg cggctcgatc cgccagcccg cctcgctctg
      181 cgggatcgtc gggctgaagc cgacctacgg cgcgatctcg cgctacggga tgatcgcctt
      241 cgcctcctcg ctcgaccagt gcgggccgct gacccgcgac gtcaccgacg cggcgctgat
      301 gctggcggcg ctggaggggc acgacccctg cgactcgacc tcggtcggga tcgaaggcgg
      361 gatcgtcgcc ccgcgggccg aggacctcaa gggcctgcgc ttcggcgtgc ccgcggagct
      421 ggccagcgac gcgatcgacg ccggcgtgcg cgaggtcttc gaggcgaccc tggcgcggat
      481 cgaggcgctg ggcggcgaga tctccgaggt caccctgccg agcgccgagc acgggatctc
      541 cgcctactac gtgatcgccc cgccgaggcc tcctcgaacc tggcccgcta cgacggcgtc
      601 cgctacggga tgcgcgccgg cggtgacggc gacctgatcg agatgtacga gacgacccgc
      661 accgagggct tcgggcccga ggtcaagcgg cgcatcctgc tcggcaccta cgcactctcc
      721 tccggctact acgacgctta ttacgggcag gcgcagaagg tgcgcacgcg gatcgccgag
      781 gacttccgcg gcgccttctc cggggtcgac ttcgtcgtca ccccgacctc gccctcggtc
      841 gccttcggcc tcggcgagaa gaccgccgac ccgctggaga tgtacctcaa cgacttcttc
      901 accgtgccga tgagcctcgc cgggatcccg gcgatctcga tccccgccgg tctcgccgcg
      961 cccgagggcg gcggcgccaa gctgccggtc gggttccaga tcgccgcgcc cgccttcgcc
     1021 gagcagaagc tgctggaggc ggcgttcgcg ctggagaccg cgatcgactt cgccggcgcc
     1081 acgcccggca tcccgtacct gaacggggga ggtgagccgg cgtgagcgac ctcgaagcgg
     1141 tgatcgggct ggagatccac gtccagctgg cgaccgagac gaagatgttc tgcggctgcg
     1201 aactctcctt cggcgacgag ccgaacaccc acacctgccc gatctgcctc gcccatcccg
     1261 gcgtgctccc ggtcgccaac gagcaggcga tcctctacgc cctgcagatc ggcctggcgc
     1321 tggagtgcga gatcgccccg cgctcgatct tccaccgcaa gaactacttc tatcccgaca
     1381 gcccgaaggc ctaccagatc agccagtacg acatcccgat ctgcggcgcc ggccggctgg
     1441 gcgacgtgcg catccaccga gcccacctcg aggaggacgc ggcgaagacg atccacctcg
     1501 gtgagtcggg ccggatccac ggctccgccc actcgctggt cgacttcaac cgcggcggca
     1561 cgccgctggt cgagatcgtc accgagccgg acctgcgcga cgccgccgaa gccgcccagt
     1621 gggcgcgcct gctgcgcgag acggtgcgcc agctcggcgt ctccgacgtg aacatggagg
     1681 agggcagcct gcgggtcgac gccaacgtct ccgtgcgccc ggtcggctcg gaggagctcg
     1741 ggaccaagac cgagctgaag aacatgaaca gcttccgctt cctgcagcgc gggatcgaag
     1801 ccgagctgga gcgccagaag gcgctgattg ccgccggcgg ggaggtcgaa caggagacgc
     1861 tccacttcga cccccgcagc ggcgagctga cctcgctgcg ctcgaaggag gaagcccacg
     1921 actaccgcta cttccccgag ccgtacctgg tcccgatcgt gcccaccgag gagatgctgc
     1981 gcgaggcgcg cgaggcgctg ccggagctgc ccgcggcgcg gatcgcccgc tacgaaggcg
     2041 acggcctcag caccgacgtc gccaccgtcc tggcgaccga cccggagacc gccggctact
     2101 acgagcaggt cgccgcggcg gcgagcgggg tggaggcgcg cgtggtcggc aactgggtga
     2161 ccggtgagct ggtcgcggcc ctgcgcgccg ccgaggccga gggtggcgca accgcctcca
     2221 aggtgacccc ggacgcgctc gccgccctga tcggcttggt cgccgagaag aagatctccc
     2281 acggcagcgg aaaaaccgtg cttgccgcac tggtcgagga ggggggagac cccgccgcga
     2341 tcgtcgagcg cgagggcctc gcccagatgg ccgcggacga cggcgagctg gaggcgatcg
     2401 tcgccgccgc gatcgaggcc aaccccgacg ccgccgcgaa gatccgcgaa ggcaacaaca
     2461 aggcgatcgg cgcgatcgtc ggccacgtga tgcgcgagac caagggccgc gccgacggcg
     2521 gcgaggtcaa ccgcctgatc gcccaacagc tcaactcctg agtcgacata acgtgcgttc
     2581 tcgcactttg taacaacttg ttacaaagtg ctaaaatccc agctatggct tggaatgtcg
     2641 tcatagctgg aggcggtttc ggcggagcgg cagccgcccg cgagctggag cgcgtgatgc
     2701 cgaagcagtc ggcgcggctg gtgatggcaa acgagcagaa cttcgctctg tacacgccgt
     2761 tcctgccgga ggccgcggcg ggcaccttgg agccgcggca cgtgaccacg ccgctgcgcg
     2821 acatcttgaa gcgcacctac ctgcggctcg gctcggtcgt cggccacgac cccgaggcga
     2881 agaccgtgca gctggcgccc aagcacgggc cgctggagac gcttccctac gaccagctgc
     2941 tgctcagcct cggctcggtc tcgcgggtcc tgccagtgcc cggcctggcc gagcacgcgg
     3001 tcggcttcaa gtccctggcc gacgcgatct acctgcgcaa ccacgtgatc gaaacgctcg
     3061 aggaagcgaa cgcgaccgag gaccccgcgc ggcgcgacga actgctcacc tacgtcttcg
     3121 tcggcggcgg ctacgccggc ctcgaggcgc tggccgagct gcaggatttc gccgccgacg
     3181 cgatggaccg ctacccgcgc gcccgcctgc acgggatgcg ctgggtgctg gtcgaggccg
     3241 ccgaccgggt gctgccggag atcgacctgg agctggcgga atatgcgctg cgcgagctgc
     3301 ggggccgcgg catcgacatc cgcatggaga cgacgctgga ggaggtcggc gccgacttcg
     3361 cgcgcctctc cagcggcgag acgctcgcca cccagaccgt cgtctggacc gccggcgtcg
     3421 ccccgcagcc gatcctcaaa cagctcaacc tgccgcttga cgagcgcggc cgggtcccgg
     3481 ttgactccca cctgcgggtc gaggggctcg actcggtctg ggcgatcggc gactgcgccg
     3541 ccgctcccga cccgcgcggc ggcctctgcc cgccgaccgc ccagatcgcc gtgcgccagg
     3601 ggccggtcgc ggcgcgcaac atcgccgctg agctgggcat cggttcggcc cgacccttcg
     3661 agtaccgctc agaagcctcc ttcgtcaacc tcggccgcta caaggcggtc ggccggatcg
     3721 gcgagcgaac cttccgcggc ttcccggcct ggtggctggc ccgcagctac cacatgtccc
     3781 agattcccgg caccgcccgc aaggcccgcg cggtgctcga ctggaccgcc agcctgccgt
     3841 tcaagcgcga catctccgag gtcggctcga tcgggcaccc gaagccgctg gtctagggcg
     3901 tcaccgtcag cgttgctttc atccccgcgg cctcgtggcc gtcgaggtcg caccagaggc
     3961 ggtattcgcc cgcgggaagg ttgaatttcg ctttgccctg gacgcccggg ccgaccgtgg
     4021 tgatcttctc ttccggcccg cttgcgtcct cgagctgcaa gttgaggttg tgcgggtcct
     4081 cgcccatgtt gttgagctcg accacgacct cgccggccgg cacttccggg cgcgagagcg
     4141 tgtaggacca ctcgaccgct ttcaccccga gccgcgggat cgtcgggtct tccggttcgg
     4201 gctgcggttc ggtcggcggc gggacgacca ccggcggcgt gaccggcgcc ggcgccgccc
     4261 gcttcgcaca ggtccgccag cgcttcaccc gcacgacttt cttgcgcttc ccgtcctttt
     4321 tcacccactt gaccacccgc ttgtggtgct tcttccaggc cacgcagggg gtcgccgcac
     4381 cgccgccggc cggcagcagc aggaccgccg ccagcgccac gcaggccgcg gccagggccg
     4441 cgcgcctcac gctttcacca aggtcggctg gatgaagccg tccgcgccgg ggatgatcgg
     4501 gccggcgtcg tgcccgagcc actgctcgag cagcgcgcag tacatgccgc ggaagtcgct
     4561 ggtgacgcgc aggttgtcgt agttgtcgag tttggccagg ccggggaact cgccgaccat
     4621 ctcgccttta gctttcgagc cgatcacgaa ggcgcagccg gcggcgccgt ggtcggtccc
     4681 ggccgagccg ttctcctccg gccggcggcc gaactcgctc cacatctcga tcatcacccg
     4741 gtcgtcgagc ccgcgcgcct cgaggtcgcg ctggaaggcg agcacgccgt cgatcgtctc
     4801 gccgaggttg cggtcgaggt cttcggcctg gccggcgtgg gtgtcgtagc cgccggcggc
     4861 gcggatcgtc acgacccgca tcggcagccc gaagtcgatg taggcggcga gcccggcgag
     4921 cttgtgggcg aaccaggtgt cgggataggc gaccgggctg gagaagtcgc cgacgccgcc
     4981 gaggtcctcg cgcagcttgt cggtctgggc ggtcgcgcgg cgcacctgcg agagcccttt
     5041 cgagtcggcc ggcagtgagc cgaacttggc gaagcttttg tacatctccg cccgcaccgg
     5101 gtcggagacc gaggaccaga ggtcgtagcc gtcgacgctg tcgatcgccg ccaccggctt
     5161 gtcggcggtt gcgatcatcg gc
//
LOCUS       DASRIG010000559         6000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_1038148, whole genome shotgun sequence.
ACCESSION   DASRIG010000559 DASRIG010000000
VERSION     DASRIG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038148"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFB52_14885"
     CDS             <1..1176
                     /locus_tag="VFB52_14885"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HZO07672.1"
                     /translation="VAADTLLDVSGLPKTIEFDPGDGAVEASANVSLHEFLAALGERG
                     KSVSATGGTDHQTLAGLISTGTAPASSRHALYDLLEWVELVTVEEASGRAVEKRIEQG
                     DPEFAAVVCSLGLLGVLTRVRFRTVEELFFDVVMKVVRLDEVLTDLDATSARYDFWRI
                     NWMANSDKALLWAATAVPAEDSRPDGDYDTEPGEQILDKVFEAMDEIFDTGPLLNFGL
                     ERVYEVLALTYDDSEFTGPLRNMLPVDRRAPLHVAMAEWSFRPRDLQRVIGECEQYFE
                     EQGWPNIPTEIELTRVDDRMMSPWNWTGLPYIVKLNFMYLTEVCKEPGEKEGIHAHLR
                     GLWQHLQAAGVPFKAHWGKINFIDPEFARRNYELDAFAPLISPLFMNDYLEERIGDA"
     gene            1169..2338
                     /locus_tag="VFB52_14890"
     CDS             1169..2338
                     /locus_tag="VFB52_14890"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZO07673.1"
                     /translation="MLESAPGGGAPDAPETFPGLSDMHAHPAVNSFLWGRDLRKHYWT
                     GGRFDPLASLTDFKMLRRGGVKVLWSSVYVPEKPYLAVPPIHLLALILPSGRKLLKHS
                     SWECLLMMMDNMERQVGRVDDFEIVRSNQELDAARAADKTAIVHTVEGGHTLDNGLAD
                     DDVDGRLERLGVLAERGVASITIAHHYPNQLAGHAIGIPKEQLKLLFWRLPIEVDPER
                     GLTPIGAAVIERMIELRIVPDVAHCTTKARRDIYELVADRIPIVATHVGVQSMNSAPY
                     NLDRIDAEAIASSGGVAGVIFMPYWLEETDPGDGREAIWRTMETLHDWTGSWRHVGLG
                     TDFDGFTNPADDWDSSAKLPAVREMLGEKGLAEDDTKALLGGNARRVLRDGWRWD"
     gene            complement(2335..3189)
                     /locus_tag="VFB52_14895"
     CDS             complement(2335..3189)
                     /locus_tag="VFB52_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07674.1"
                     /translation="MEEVVKKLFSGNLRVMVVAVIVAVVVGGSAYAAGAIDGRSLKPN
                     SVALSKLTKQARQQLSQPGPAGPQGVAGPQGPQGLPGDAGPQGAAGKDGVDGEDGDDG
                     AAGEDGEDGLDGEDGDDGEDGKDGEQGPPGPTEHSYGVSALYLDGTYVPASASWTPTV
                     PRDHNNAAQSSGSTVITCAATCELTSRAVIRTDDPTLTGQAGGGVLVTGVDGALVAAG
                     KTPANPLYGNSSVVDVATVGLGSKAPTQPALTGTEVPIEWTFGPDELPAGTYLVSGTI
                     QFFEFIEP"
     gene            complement(3349..4119)
                     /locus_tag="VFB52_14900"
     CDS             complement(3349..4119)
                     /locus_tag="VFB52_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07675.1"
                     /translation="MPELAIHTVGSGPPLLLVNGYASTGVDWDPTFLAALGERHEVIC
                     PDNRGMGGSPLGEAELTIEAMAADLEALLDARGIERLPVAGWSMGGFAAQALARRAPG
                     RVTALALIATDPGGPDAVAAEPAVWAQLTDRSGSAREQASRLIPLLFPAALAPAIDAE
                     FGELIAAGRAAMSPAALDAQETAMAAWHVDGVGEAAGTAPPTVVIHGEADVVIPTANA
                     ERLAAHWPGARVELIPGAAHAVMAQEPQRVAAAIAGLS"
     gene            4103..4483
                     /locus_tag="VFB52_14905"
     CDS             4103..4483
                     /locus_tag="VFB52_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07676.1"
                     /translation="MASSGTGPTIPIAPDEKELAVIISVLTRRLKEGKDYSDFRAAWL
                     PDKGFGVPTRVVSAVSLEDPREVVTIGFTDLDPADAEAFLARIGPQEKLRHERLEEVL
                     EPGGTNAVYIQVADDDLTNEPPAE"
     gene            complement(4487..5632)
                     /locus_tag="VFB52_14910"
     CDS             complement(4487..5632)
                     /locus_tag="VFB52_14910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZO07677.1"
                     /translation="MRAAIVVAGLALSAVAFAGCMDGTVRDPPGEATRGKQQGGGKQA
                     GGGKEGGDGEKSGGGQGDGISFAAVGDTAMGITPILPPSPSTYFDAIAGELRGDVVFG
                     NLEGTLTDVDEDVKCGGADPGTCFAFRAPPEYAKYLADAGFNVMSLANNHSYDFGEAG
                     QEETVGALYRAGIEPAGIPGRLVFIDVDGTKVSFLGFASYDYTNSLTDLEAARALIRQ
                     ARKRSDIVVVVNHAGAEGSDAHHVTGETEIYLGEDRGNPEEFAHMAVEAGADLVLGSG
                     PHVLRGMEIYRDRLIAYSLGNFSGYDNFSLEGILADTVVLHATLGPDGRFRSGRLASV
                     RMIEDGRPVPDPEGSGAALIEELSQDDFAPSKAIRVGADGKIEAPQG"
     gene            complement(5629..>6000)
                     /gene="dtd"
                     /locus_tag="VFB52_14915"
     CDS             complement(5629..>6000)
                     /gene="dtd"
                     /locus_tag="VFB52_14915"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZO07678.1"
                     /translation="EGDRVAQIGPGMLVLLGVRVGDGEAECDRLAEKVAKLRIFDDAE
                     GRMNEPLGEREILCVSQFTLYGDAHKGNRPSYVQAARPEDAEPLYDRFCERLGAKKGV
                     FGACMGVALINDGPVTLMVEI"
BASE COUNT          935 a         2170 c         1995 g          900 t
ORIGIN      
        1 gtcgccgccg acaccctgct cgacgtctcc ggcctgccga agacgatcga gttcgacccc
       61 ggcgacggcg ccgtcgaggc cagcgccaac gtctccctgc acgagttcct cgccgcgctc
      121 ggcgagcgcg gcaaatcggt cagcgccacc ggcggcaccg accaccagac gctcgccggg
      181 ctgatctcga ccgggaccgc cccggccagc tcccgccacg cgctctacga cctgctcgag
      241 tgggtcgagc tggtcaccgt cgaggaggcc agcggccgcg cggtcgagaa acggatcgag
      301 cagggggacc ccgagttcgc cgccgtcgtc tgctcgctcg gactgctcgg cgtgctcacc
      361 cgggtccgct tccgcaccgt cgaggagctc ttcttcgacg tggtgatgaa ggtcgtccgc
      421 ctcgacgagg tgctgaccga tctcgacgcg acctccgcca ggtacgactt ctggcggatc
      481 aactggatgg cgaatagcga caaggcgctg ctctgggcgg cgaccgccgt gccggcggag
      541 gattcacgcc ccgacggcga ctacgacacc gaaccgggtg agcagatcct cgacaaggtc
      601 ttcgaggcga tggacgagat cttcgacacc ggcccgctgc tcaacttcgg gctcgagcgc
      661 gtctacgagg tgctcgccct cacctacgac gacagcgagt tcaccggccc gctgcgcaac
      721 atgctgccgg tcgaccggcg cgcgccgctg cacgtggcga tggccgagtg gagcttccgc
      781 ccacgcgacc tgcagcgggt gatcggcgag tgcgagcagt acttcgagga gcagggctgg
      841 ccgaacatcc cgaccgagat cgagctgacc cgggtcgacg accggatgat gtctccctgg
      901 aactggaccg gcctccccta catcgtcaag ctcaacttca tgtacctgac cgaggtctgc
      961 aaagagccgg gcgagaagga gggcatccac gcccacctgc gcgggctctg gcagcacctg
     1021 caggcagccg gcgtcccgtt caaagcccac tggggcaaga tcaacttcat cgacccggag
     1081 ttcgcgcggc gcaactacga gctcgacgcg ttcgcgccgc tgatctcgcc cctgttcatg
     1141 aacgactacc tggaggagcg aatcggcgat gcttgagtcc gcccccgggg gcggtgcccc
     1201 tgacgccccc gagaccttcc ccggcctctc cgacatgcac gcccacccgg cggtcaactc
     1261 gttcctctgg ggccgcgacc tgcgcaagca ctactggacc ggcggccggt tcgacccact
     1321 ggccagcctc accgacttca agatgctgcg caggggcggc gtcaaggttc tctggtcctc
     1381 cgtctacgtg cccgagaagc cctacctcgc ggtgccgccg atccatctgc tggcgctgat
     1441 cctcccctcc gggcgcaagc tgctcaagca cagctcctgg gagtgcctgc tgatgatgat
     1501 ggacaacatg gagcgccagg tcgggcgcgt cgacgacttc gaaatcgtcc gctccaacca
     1561 ggagctcgac gccgcgcggg cggcggacaa gaccgcgatc gtccacaccg tcgagggcgg
     1621 ccacacgctc gacaacggcc tcgccgacga cgacgtcgac ggacggctcg agcggctggg
     1681 cgtgctcgcc gagcgcggcg tcgcctcgat cacgatcgcc caccactacc ccaaccagct
     1741 cgccggccac gcgatcggca tccccaagga gcagctgaag ctgctgttct ggcgcctgcc
     1801 gatcgaagtc gaccccgagc gcgggctgac cccgatcggc gccgcggtga tcgagcggat
     1861 gatcgaactg cggatcgtcc ccgacgtcgc ccactgcacg acaaaagccc gccgcgacat
     1921 ctacgagctg gtcgccgacc ggatcccgat cgtcgccacc cacgtcggcg tgcagtcgat
     1981 gaactcggcc ccctacaacc tcgaccgcat cgacgccgaa gcgatcgcct cctcaggcgg
     2041 cgtcgccggc gtcatcttca tgccctactg gctggaagaa accgaccccg gcgacgggcg
     2101 cgaggcgatc tggcgcacga tggagacgct ccacgactgg accggctcct ggcgccacgt
     2161 cgggctgggc accgacttcg acggcttcac caatcccgcc gacgactggg acagctcagc
     2221 gaagctgccc gccgtccgcg agatgttggg ggagaaaggc ctcgcggagg acgacaccaa
     2281 ggcgttgctg gggggcaacg cacggcgcgt cctccgcgat ggctggcgct gggactaggg
     2341 ctcgatgaat tcgaagaact ggatcgtgcc ggagacgagg tacgtcccgg ccggcagttc
     2401 gtcgggtccg aaggtccatt cgatcggcac ttcggtgccg gtcagagccg gttgggtcgg
     2461 cgctttcgag ccgagcccga cggtagccac gtcgaccacc gaggaattgc cgtagagcgg
     2521 gttggcgggc gttttaccgg ccgcgaccag ggcgccgtcc accccggtga cgagcacgcc
     2581 accgccggcc tggccggtca acgtcgggtc gtcggtgcgg atcacggcac gcgaggtcag
     2641 ctcgcaggtc gccgcgcagg tgatcaccgt gctgcccgag ctctgcgccg cgttgttgtg
     2701 gtcgcgcgga acggtcggag tccagctggc gctggcaggg acgtaggtgc cgtcgaggta
     2761 gagggcgctg acgccgtagc tgtgctcggt cggcccgggc ggaccctgct caccgtcttt
     2821 cccatcttcg ccatcgtcac cgtcttcacc atcgaggccg tcttcgccgt cttcgccggc
     2881 cgcaccgtcg tcgccgtctt ctccatcaac tccgtctttg ccagccgctc cctgcgggcc
     2941 ggcgtcaccg gggagcccct gcgggccctg cggaccggcc acgccctgcg gtcccgcggg
     3001 tccgggctgg gacagctgct ggcgggcctg cttggtcagc ttgctgagcg cgaccgagtt
     3061 gggcttcagc gagcgcccgt cgatcgcgcc ggcagcgtag gccgagccac ccaccaccac
     3121 cgcgacgatc accgcgacga ccatcacgcg gagatttcca gagaagagct tcttcacgac
     3181 ttcctccatc catttagaga actaagcagt tcttgaagtc agttcgggaa gctgggggaa
     3241 gcggcgcaaa tacgcaatcg aacccaacag atgttgtaaa cactcaggtt tttaagataa
     3301 gaattccttt cctgtccaaa aaacggaagc gacacaggtg aaaccggctc agctcaggcc
     3361 ggcgatcgcc gccgcgacac gctgcggctc ctgcgccatc accgcgtggg cagcgccggg
     3421 aatcagctca actcgggcgc cgggccagtg cgcagcaagg cgctcggcgt tggcggtggg
     3481 gatgaccacg tcggcctcgc cgtggatgac gacggtcggc ggcgccgttc cggccgcctc
     3541 ccccactccg tccacatgcc aggcggccat tgcggtctcc tgtgcgtcga gcgcggcggg
     3601 cgacatcgcc gcccgccctg ccgcgatcag ctcgccgaac tcggcgtcga tcgccggtgc
     3661 cagcgccgct gggaaaagca gggggatcag ccgcgaggcc tgctcgcggg cgctgccgga
     3721 gcggtcggtc agctgcgccc agaccgccgg ctcggcggca accgcgtcgg gcccgccggg
     3781 gtcggtcgcg atcagcgcca gggccgtgac ccgcccgggc gcccgccgcg ccagcgcctg
     3841 cgcagcgaag ccgcccatcg accagccggc gaccggcagc cgctcaatcc cgcgcgcgtc
     3901 gagcagcgcc tcgaggtcgg ccgccatcgc ctcgatcgtc agctccgcct cgccgagcgg
     3961 cgagcctccc atgccgcgat tgtcggggca gatcacttcg tgacgctcgc cgagcgcggc
     4021 aagaaaggtc gggtcccagt cgaccccggt cgaggcgtag ccgttgacga gcagcagcgg
     4081 cgggccgctg ccgacggtgt ggatggcgag ttccggcacc gggcctacta tcccgatcgc
     4141 ccccgacgaa aaggagctcg ccgtgatcat ctccgtcctc acccgccgcc tcaaagaggg
     4201 caaggactac tccgacttcc gcgccgcctg gctgcccgac aaggggtttg gcgtgcccac
     4261 ccgcgtggtc agcgcggtca gcctcgagga tccgcgcgag gtggtcacga tcggcttcac
     4321 cgacctcgac cccgccgacg ccgaggcctt cctcgcgcgg atcggtccgc aggagaaact
     4381 ccgccacgaa cgactggaag aggtgctgga gccgggcggc accaacgccg tctacatcca
     4441 ggtcgccgac gacgacctca ccaacgagcc acccgcggag tgaggctcag ccctgcgggg
     4501 cttcgatctt gccgtccgcg ccgaccctga tcgccttcga gggcgcgaag tcgtcctggg
     4561 agagttcctc gatcagggcg gcgccggagc cttcggggtc gggaaccggc cgcccgtctt
     4621 cgatcatccg caccgaggcg aggcggccgg agcggaaacg gccgtcgggc ccgagggtcg
     4681 cgtgcaggac gacggtatcg gcgaggatcc cttcgaggct gaagttgtcg tacccggaga
     4741 agttgccgag gctgtaggcg atcaagcggt cgcggtagat ctccatcccg cgcagcacgt
     4801 gcggcccgga gccgagcacg aggtcggcgc cggcctcgac cgccatgtgc gcgaactcct
     4861 ccgggttgcc gcggtcctcg ccgaggtaga tctcggtttc gccggtcacg tggtgggcat
     4921 cggaaccctc ggccccggcg tggttgacga cgacgacgat gtccgagcgc ttgcgcgcct
     4981 ggcggatcag cgcccgcgcc gcttccaggt cggtgagcga gttggtgtag tcgtaggagg
     5041 cgaagccgag gaaggagacc ttggtcccgt cgacgtcgat gaagaccagc cgccccggga
     5101 tcccggccgg ttcgatcccc gcccggtaga gcgccccgac cgtctcctcc tggccggctt
     5161 cgccgaagtc gtaggagtgg ttgttggcca gggacatcac gttgaacccg gcgtcggcca
     5221 ggtacttcgc gtactcgggc ggggcgcgga aagcgaagca ggttcccggg tcggcgccgc
     5281 cgcacttgac gtcttcgtcg acgtcggtga gcgtgccctc gaggttgccg aagacgacgt
     5341 cgccgcggag ctcgccagcg atcgcgtcga agtaggtcga cggagagggc ggcaggatcg
     5401 gcgtgatccc catcgcggtg tcgccgacgg cggcgaagga gatgccgtct ccctggccac
     5461 ccccggactt ctcgccgtcc ccaccctcct tgccgccgcc ggcctgcttg cccccgccct
     5521 gctgctttcc tcgggtggct tcgccgggcg ggtcgcggac ggtgccgtcc atgcaacctg
     5581 cgaacgcgac cgcgctcagc gcgagacccg ccacgacgat cgccgccctc atatctcgac
     5641 catcaaggtc accgggccgt cgttgatcag ggcgacgccc atgcaggcgc cgaacacgcc
     5701 cttcttcgcc cccagccgct cgcagaagcg gtcgtagagc ggctcggcgt cctcgggccg
     5761 cgccgcctgc acgtagctcg gccggttgcc cttgtgggcg tcgccgtaga gcgtgaactg
     5821 gctgacgcag aggatctccc gctcgcccag cggctcgttc atccggccct cggcgtcgtc
     5881 gaagatccgc agcttcgcca ccttctcggc gagccggtcg cactctgcct cgccgtcgcc
     5941 gacccgcacc cccagcagca ccaacatccc agggccgatc tgggcgactc gatcgccctc
//
LOCUS       DASRIG010000560         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2080468, whole genome shotgun sequence.
ACCESSION   DASRIG010000560 DASRIG010000000
VERSION     DASRIG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080468"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1380)
                     /locus_tag="VFB52_14920"
     CDS             complement(16..1380)
                     /locus_tag="VFB52_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07679.1"
                     /translation="MPAREFDYVSDKTTPLKGVDKSTLLNLLWGDRVEVLEDGDPWVK
                     VRARGRDTVGWVKKTALGGEPLLEVYFIDVGQGDGILIKTPGKKHLMVDGGNLRKAQN
                     PRKNAADFVDWKFFHDYGEDKIELEAVIASHCDVDHYGGLTDLLAVDKNDELDCKEVR
                     VETIYHAGLGYWSTGDGEKTLGPTEPKDGKRFFRQLVGNREEVEAALQPDAEPALAGE
                     WAKFMQAAADTLNAGGDPAAIERLSDRTEHLNGFGPGVDGEVAIKVLGPLEFEVEVGG
                     KTLHARQEYDGEAGISTNGNSVLLRLDYNGCRILLTGDLNTESQEALLEFYEGKAGEL
                     ACDVAKACHHGSEDVSIPFLQALEPAATVISSGDNEGHDHPRPKVISASALTGYRHPE
                     DGKTLESPLIFSTELSRSVKLGPPVELVYGLVNVRTDGKEILFATRDEHTRRWRTDKV
                     DSRF"
     gene            1562..2452
                     /locus_tag="VFB52_14925"
     CDS             1562..2452
                     /locus_tag="VFB52_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZO07680.1"
                     /translation="MGYETILYSTEGAIATVTLNRPQALNAIVPPMPEEVERAVNEAV
                     RDPAVKVIVLRGAGRAFCAGYDFGDGFKLGEEFKTDGQWDPGKDMAAVTAAATSPHQQ
                     FMSIWRSPKPVIAQVHGWCVGGGSDYALLADIVVASEDAQIGTPYSRVWGAYLSGMWI
                     YRLGLARAKHHALTGRPLSGREAAEVELINEAVPFGQLEARVRERAEELAALPMGQLA
                     AQKLMVNQAYENMGLTATQTLGPILDGIMRNIPAAHEFVDRAESQGVPATVAERDAPF
                     GDYSKAPPEQRPNPDNVIEP"
     gene            2449..3186
                     /locus_tag="VFB52_14930"
     CDS             2449..3186
                     /locus_tag="VFB52_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZO07681.1"
                     /translation="MTAKDSKAVRATVRGRVQGVFFRQSTIERACQLMLLGWVRNEDD
                     GTVAVHIEGPTAAVDEMLVWLGEGPPGARVEEVEAETVKVEGHEQFAVRGVDAGVFVV
                     QEHQATAHHFDLRLEVDGVMKSWAVPKGPSLDPAVKRMAIEVADHDPSHNDFEGELEG
                     GAVIVWDRGGYEQGGRVPWPEAIERGHAVFVLNGEKLRGGFALQRTGPPGEKSRWLLI
                     KRKDDEARPGSDITAESPRSVLSDRTL"
     gene            3267..>3858
                     /locus_tag="VFB52_14935"
     CDS             3267..>3858
                     /locus_tag="VFB52_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07682.1"
                     /translation="MNYLRRISTRRLLTLCALTLVVAIGATAVAVATSGDGPKPEPKP
                     LANAVHDALDAPQVPGITADVEFTNNLIDSSSLEGGDPLLTGASGRVWASPADGGKLR
                     LELQASPELGGGNDSQILVDGDRFEIYDGTTNTVYRGTIEEPKGKDAEHGIPSVERIQ
                     TEIDRAGKHADLSGAEPSNIAGQPAYTVSASPSHDGG"
BASE COUNT          604 a         1309 c         1347 g          598 t
ORIGIN      
        1 agcgctccta actcctcaga agcgcgaatc caccttgtcg gtgcgccatc tccgcgtgtg
       61 ctcgtcgcgg gtcgcgaaga ggatctcctt gccgtccgtg cgcacgttga ccagcccgta
      121 gaccagctcg accgggggac cgagcttgac gctgcgggac agctcggtcg agaagatcag
      181 cggcgactcc agcgttttcc cgtcctcggg gtggcggtac ccggtcagcg cgctcgcgct
      241 gatgactttc gggcgcgggt ggtcgtggcc ctcgttgtcg ccggaggaga tcacggtggc
      301 ggccggttcc agcgcctgca ggaaggggat cgagacgtcc tcgctgccgt ggtggcaggc
      361 cttggcgacg tcgcaggcga gctccccggc tttgccctcg taaaactcga gcagcgcctc
      421 ctggctctcc gtgttgaggt cgccggtgag caggatccgg cagccgttgt agtcgaggcg
      481 aagcagcacg ctgtttccgt tggtgctgat gccggcctcg ccgtcgtact cctgccgggc
      541 gtgcagcgtt ttgcctccca cctcgacttc gaactcgagt gggccgagca ccttgatcgc
      601 cacctcgccg tcgacgccgg gcccgaagcc gttcaggtgc tcggtccgat cgctgaggcg
      661 ctcgatcgcg gccgggtcgc cgccggcgtt gagggtgtcg gccgcggcct gcatgaactt
      721 cgcccattcg cccgccaggg caggctctgc gtcgggttgc agggcggcct cgacctcttc
      781 gcggttgccg accagctgac ggaagaaccg cttgccgtct ttgggctcgg tcggccccag
      841 ggtcttctct ccgtccccgg tgctccagta cccgagcccc gcgtggtaga tcgtctcgac
      901 cctgacctct ttgcagtcga gctcgtcgtt cttgtcgacc gcgagcaggt cggtgaggcc
      961 gccgtagtgg tcgacgtcgc agtggctcgc gatcaccgcc tcgagctcga tcttgtcctc
     1021 gccgtagtcg tggaagaact tccagtcgac gaagtcggcg gcgttcttgc gcgggttttg
     1081 ggccttgcgc aggttgccgc cgtcgaccat caggtgcttt ttgccggggg tcttgatcag
     1141 gatcccatca ccctggccga cgtcgatgaa gtagacctcg agcagcggtt cgccgccgag
     1201 cgccgttttc ttcacccagc cgaccgtgtc gcggccgcgg gcgcggactt ttacccaagg
     1261 atcgccgtcc tcgagcactt ccacccggtc accccagagc aggttgagca gcgtggattt
     1321 gtcgaccccc ttgagcgggg tcgttttgtc cgagacgtag tcgaactcgc gagcgggcat
     1381 gggccgacgc tatctcaata ctcgaccctt gttgaggaac tttcgcgtag ctgcttaagg
     1441 tgtcgcgcag gcgtcccgac cgcgccgagg acaagcgcga gcagcgcgaa caggagaggc
     1501 taggtaggaa gtacccaggc agagatcagg aaaagcaggc aggaatcaac cgggaggggc
     1561 gatgggctac gagacgattc tctattcgac cgagggggcg atcgcgacgg tgacgctgaa
     1621 ccggccgcag gcgctgaacg cgatcgtgcc gccgatgccg gaggaggtcg agcgggcggt
     1681 caacgaggcg gtgcgcgacc cggcggtgaa ggtgatcgtg ctgcgcggcg ccggccgcgc
     1741 cttctgcgcc ggctacgact tcggcgacgg gttcaagctc ggcgaggagt tcaagaccga
     1801 cggccagtgg gacccgggca aggacatggc ggcggtgacc gctgcggcga cctccccgca
     1861 ccagcagttc atgagcatct ggcgctcgcc gaagccggtg atcgcccagg tgcacggctg
     1921 gtgcgtcggc gggggcagtg actacgcgct gctcgccgac atcgtcgtcg cctccgagga
     1981 cgcccagatc ggcacgccct actcgcgcgt ctggggcgcc tacctctcgg gcatgtggat
     2041 ctaccggctc ggcctcgcac gcgccaagca ccacgcgctc accggccggc cgctctccgg
     2101 ccgcgaagcc gccgaggtcg agctgatcaa cgaggccgtt cccttcgggc agctcgaggc
     2161 gcgcgtgcgc gagcgggccg aggagctggc ggcgctgccg atgggtcagc tggcggcgca
     2221 gaagctgatg gtcaaccagg cctacgagaa catgggcctg accgcgaccc agaccctcgg
     2281 cccgatcctc gacgggatca tgcgcaacat ccccgccgcc cacgagttcg tcgaccgcgc
     2341 cgagtcccag ggcgtgcccg cgaccgtcgc cgaacgcgac gcgcccttcg gcgactacag
     2401 caaggccccg cccgagcaga ggccaaaccc ggataacgtg atcgagccgt gaccgcgaaa
     2461 gactcgaaag ccgtgcgggc gacagtgcgc gggcgcgtgc agggggtctt cttccgccag
     2521 tcgacgatcg agcgtgcctg ccagctgatg ctgctcggct gggtgcggaa cgaagacgac
     2581 ggcacggtgg cggtgcacat cgagggtccg accgcggcgg tcgacgagat gctcgtctgg
     2641 ctgggcgagg ggccgccggg ggcgcgggtg gaggaggtcg aggcggagac ggtcaaggtc
     2701 gaggggcacg agcagttcgc ggtgcgcggg gtcgacgccg gcgtcttcgt cgttcaggag
     2761 caccaggcga ccgcgcacca cttcgacctg cggctggagg tcgacggcgt gatgaagtcg
     2821 tgggcggtgc cgaaggggcc ctcgctcgac ccggcggtga aacggatggc gatcgaggtc
     2881 gccgaccacg acccgtccca caacgacttc gagggcgagc tggagggcgg cgcggtgatc
     2941 gtctgggacc gtggcggcta tgagcagggc gggcgcgtcc cctggccgga ggcgatcgag
     3001 cgcggccacg cggtcttcgt cctgaacggc gagaagctgc gcggcggctt cgccctgcag
     3061 cgcaccggcc cgcccggcga gaagtcccgc tggctactga tcaagcgcaa agacgacgag
     3121 gcccgccccg gctccgacat caccgccgaa tcccccaggt cggtgctcag cgaccgcacg
     3181 ctctgagccg acagggccaa gctgtcagag ttctttcaag gagcaaacct aggctggaac
     3241 ggtcccgttg tagagaggag accgtcttga actaccttcg tcgcatatcc acccgccgcc
     3301 tgctgacgct ctgcgcgctc accctggtcg tcgcgatcgg cgccaccgcc gtcgccgtcg
     3361 ccacctccgg tgatggcccg aagccggagc cgaagccgct cgcgaacgcc gtccacgacg
     3421 cgctcgacgc gccccaggtg cctgggatca cggccgacgt cgagttcacc aacaacttga
     3481 tcgactcctc cagcctcgaa ggcggcgacc cgctgctgac cggggcctcg ggccgcgtct
     3541 gggcctcgcc cgccgacggc ggcaagctgc gcctcgagct gcaggccagc cccgagctgg
     3601 gcgggggcaa cgacagccag atcctggtcg acggcgaccg cttcgaaatc tacgacggca
     3661 cgaccaacac ggtctaccgc ggcacgatcg aggagccgaa aggcaaagat gccgagcacg
     3721 ggatcccctc ggtcgagcgg atccagacgg agatcgaccg ggccggcaag cacgccgacc
     3781 tctccggcgc cgagccgagc aacatcgccg gccagcccgc ttacacggtg agcgcctcgc
     3841 cgagccacga cggcggcc
//
LOCUS       DASRIG010000561         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2340636, whole genome shotgun sequence.
ACCESSION   DASRIG010000561 DASRIG010000000
VERSION     DASRIG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340636"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFB52_14940"
     CDS             complement(<1..376)
                     /locus_tag="VFB52_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZO07683.1"
                     /translation="MAAQREIFAASASSAERTVYEGVGEGGDHTLVIDRECEDAVFAE
                     LEALAAQGASFVAVSEERGEVAFGDGGEVRVVVDPIDGSLNARRTIPSHSLSIAVASG
                     TSMADVEFGFVHDFGADEEFVAR"
     gene            455..3397
                     /locus_tag="VFB52_14945"
     CDS             455..3397
                     /locus_tag="VFB52_14945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZO07684.1"
                     /translation="MRDPRPTATATAGERVAAHGDGPLLIVGAPGTGKTELLARRFAN
                     LAAAEGAERVLMIGSTRATARRLRERSEALLQGPFEELWIGSWDTLGERLLREHSEAA
                     GLDPFFDVLGPAERLAMLLDRLDQLPLRRHEIRGNPAGLLARLLARIDELKAASTGPT
                     SLERRAREAEEAAGSEAEREAARRDLEFAELFLAHDRILADAGSLDRGDVFLALECLL
                     VERPDLRAQLGERFRHLMVDELEEATVAQRTLLRSLAADNPNQLFALEDDSETELWFR
                     ELHPGGEVVVLEHRFRDPELGFWSCANERAQAQAVARAAEQLVVTGTAPEQICVLFAD
                     PGTQGGAVAAAMEERGVPFHLSGATALFQRPEVRDAIAWLRVLADPDDSAAAARALTR
                     PPIELRSGDLARLTTISRRRKLDLVAGCEAALESPQFQPEARERISVFLRIYNAAAAV
                     MEERRADVFVRRLIENVGLRRQRLFAAQPEVAERLLGLSRLAELATAWARREPHGSTR
                     GFVAYLSAVVEAGVEPTEGEDPRSRGAVQGMAVETVKGIGFDHVFVLGLEEEGEWSRI
                     GWPARDGLVLSRLRRGEEGEARPARYFERALAAAEAREELHEEELFGPAEGLHATYRK
                     LREQVLEASWKAGRELSEPRLDTAIDVNRAIARYLELLKLAALAQRPGEEATSEAIQA
                     VNGLLRQVATPEQQAELDASPLDSYLLGSETDRDRRLELIAAREEPSLEAFLPRRGDG
                     MLSLSASDLSLYLTCPLKYKFARVFGIPQEPTINQRFGILFHNVLERFHKEPPDHDED
                     GLRILNNLFETGWRRTGFGSSDDELQFRDRAREALRLYWEQERVAEGEPVWLERKFDF
                     KVGEHHVRGRVDRVDRLPDGDYELIDYKTGERKTEADLESDLQLALYRLAAREAWDVE
                     ASTGSYYYVLDGDKVTAPTKPDDAERVERTVLQVGEGILGQDFEPRPSPSVCSWCDYR
                     LICPAAEA"
     gene            complement(3407..>4448)
                     /locus_tag="VFB52_14950"
     CDS             complement(3407..>4448)
                     /locus_tag="VFB52_14950"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO07685.1"
                     /translation="RVRDAGDRPRRPDPALPGRLRLLGRAGAIPAAALASARRNWLVW
                     AAVIPVAIWTFLRVLGLDHGFPLATAMPFTPYVAVAALLTAGVAVALENWAAAAVAAF
                     ATFCLAAAVLPRAIGDGTVEAAGRETLDVLSTNILRGRADAEAVVALVERHDPDLLSV
                     QELTPRFARELRAAGLGELLPHELLEVAQGSAGSGLYSRLPLRPLPGPDQFAFRMPRA
                     ELTMPDGRRVRVVGVHPYPPQPNNVGVWEESLASLPSAGRGAPWVLAGDFNATLDQSQ
                     FRDLLDRGYRDAGAVAGEGLEPTFPRLGHLIPPITIDHVLADRRLDVVEYSVGEMPGS
                     DHRPVHAVLALP"
BASE COUNT          626 a         1620 c         1645 g          557 t
ORIGIN      
        1 ggcgcgcgac gaactcctcg tcggcgccga aatcatggac gaagccgaac tcgacgtcgg
       61 ccatcgaggt cccggaagcg acggcgatgc tgaggctgtg cgacgggatc gtgcggcggg
      121 cgttgagcga gccgtcgatt gggtcgacga ccacgcgcac ctcgccgcca tcgccgaagg
      181 cgacttcgcc gcgctcctcg gagacggcga cgaacgaggc gccctgcgcg gccagcgcct
      241 cgagctcggc gaagaccgcg tcctcgcatt cgcggtcgat caccagggtg tgatcgccgc
      301 cctcgccgac cccttcgtag acggtccgtt cggcgctgct cgcgctcgcc gcgaagatct
      361 cgcgctgggc cgccaccgaa cgacggcaga tccccagcca gtctgcggat aaaacgttca
      421 ccggccggat cgtatggcgc gctagcgttg ggcgatgcgc gacccccgtc caaccgccac
      481 cgcgacggcc ggcgagcgtg tcgccgcgca cggcgacggg cctctgctga tcgtcggcgc
      541 acccggcacc ggtaagaccg agctgctcgc ccggcgcttc gccaacctgg ccgcggccga
      601 gggcgccgag cgggtgctga tgatcggctc gacccgggcg acggcgcggc gcctgcgcga
      661 acgttcggag gcgctcctcc agggaccgtt cgaggagctc tggatcggca gctgggacac
      721 gctcggcgag cggctgctgc gcgagcactc cgaggccgcc ggcctcgacc ccttcttcga
      781 cgtgctcggc ccggccgagc ggctggcgat gctgctcgac cggctcgatc agctgccgct
      841 gcgccgccac gagatccgcg gcaacccggc cgggctgctg gcccgcctgc tggcccggat
      901 cgacgagctc aaggccgcgt ccaccgggcc gacctcgctg gagcggcgcg cccgcgaggc
      961 ggaggaggcg gcgggcagcg aggccgagcg cgaggcggcg cgacgcgatc tcgagttcgc
     1021 cgagctcttc ctcgcccacg accgcatcct cgccgacgcc ggcagcctcg accgcggcga
     1081 cgtcttcctc gcgctcgagt gcctgctggt cgagcgcccc gacctgcgcg cccagctcgg
     1141 cgagcgcttc cgccacctga tggtcgacga gctcgaggag gcgacggtcg cccagcgcac
     1201 cctgctgcgc tcgctcgccg ccgacaaccc gaaccagctg ttcgcgctcg aggacgacag
     1261 cgagacggag ctgtggttcc gcgagctgca cccgggcggc gaggtggtcg tgctcgagca
     1321 ccgcttccgc gaccccgagc tgggcttctg gagctgcgcc aacgagcgcg cccaggcgca
     1381 ggcggtcgcc cgcgccgccg agcaactggt cgtcaccggg accgcgccgg agcagatctg
     1441 cgtgctgttc gccgaccccg gcacccaggg cggcgcggtt gccgcggcga tggaggagcg
     1501 cggcgttccc ttccacctct ccggcgccac ggcgctgttc cagcggcccg aggtgcgcga
     1561 cgcgatcgcc tggctgcggg tactcgccga ccccgacgac tcggcggctg cggcgcgggc
     1621 gctgacccgg ccaccgatcg agctgcgctc cggcgacctc gcgcggctga ccacgatctc
     1681 gcgccggcgc aagctcgacc tcgtcgccgg ctgcgaagcg gcgctcgaaa gcccgcagtt
     1741 ccagcccgag gcgcgcgagc ggatcagcgt cttcctccgt atctacaacg ctgccgcggc
     1801 ggtgatggag gagcgccgcg ccgacgtctt cgtgcgccgc ctgatcgaga acgtcggcct
     1861 gcgccgccag cggctcttcg ccgcccagcc ggaggtcgcc gagcggctgc tcgggctctc
     1921 gcgcctggcc gagctggcga ccgcctgggc gcggcgcgag ccgcacggct ccacgcgcgg
     1981 cttcgtcgcc tacctcagcg ccgtggtcga ggccggggtc gagccaaccg agggcgagga
     2041 cccgcgctca cgcggcgccg tgcaggggat ggcggtcgag acggtcaagg ggatcggctt
     2101 cgaccacgtc ttcgtgctcg ggctcgagga ggagggcgag tggtcgcgga tcggttggcc
     2161 ggcgcgcgac gggctggtgc tctcgcggct gcgccgcggc gaggagggcg aggcccgtcc
     2221 ggcgcgctac ttcgagcggg cgctggccgc cgccgaggcg cgcgaggagc tgcacgagga
     2281 ggagctgttc ggcccggccg agggcctgca cgcgacctat cgcaagctgc gcgagcaggt
     2341 gctggaggct tcttggaagg cggggcgcga gctctccgag ccgcgcctgg acacggcgat
     2401 cgacgtcaac cgggcgatcg cccgctacct cgagctgctg aagctggcgg cgctggcgca
     2461 gcgtcccggt gaggaggcga cctcggaggc gatccaggcg gtcaacgggc tgctgcgcca
     2521 ggtggcgacg cccgagcagc aggccgagct cgacgcctcg ccgctcgact cctacctgct
     2581 tggctccgag accgaccgcg accgccgcct ggagctgatc gccgcccgcg aggagccctc
     2641 gctggaggcc ttcctgccgc gccgcggcga cggcatgctc agcctctcgg cctccgacct
     2701 cagcctctac ctgacctgcc cgctgaagta caagttcgcc cgcgtcttcg ggatcccgca
     2761 ggagccgacg atcaaccagc gcttcgggat cctcttccac aacgtgctcg agcgcttcca
     2821 caaggaaccg cccgaccacg acgaggacgg gctgcggatc ctcaacaacc tgttcgagac
     2881 cggctggcgg cgcaccggct ttggctccag cgacgacgag ctgcagttcc gcgaccgcgc
     2941 ccgcgaggcg ctgcggctct actgggagca ggagcgggtg gccgagggcg aaccggtctg
     3001 gttggagcgc aagttcgact tcaaggtcgg cgagcaccac gtgcgcggcc gcgtcgaccg
     3061 ggtcgaccgg ctgcccgacg gcgactacga gctgatcgac tacaagaccg gcgagcgcaa
     3121 gaccgaggcg gacctagaga gcgacctgca actggcgctc taccggctgg cagcgcggga
     3181 ggcctgggac gtcgaggcga gcaccggctc ctactactac gtgctcgacg gcgacaaggt
     3241 caccgctccg accaagcccg acgacgccga gcgggtcgag cgcaccgtgc tgcaagtcgg
     3301 cgaggggatc ctcgggcagg acttcgagcc gcggccctcg ccgagcgtct gcagctggtg
     3361 cgactaccgg ctgatctgcc cggccgccga agcctgacga cgcgctctac ggcagggcga
     3421 gcacggcgtg gaccgggcgg tggtcggatc ccggcatctc cccgaccgag tactcgacca
     3481 catcgaggcg gcggtcagcg agcacgtggt cgatcgtgat cggcgggatc aggtggccga
     3541 gccgggggaa ggtcggctcc aggccctcgc cggcgaccgc cccggcgtcg cggtagccac
     3601 ggtcgagcag gtcgcggaac tgcgactggt cgagcgtggc gttgaagtcg ccggccagca
     3661 cccagggggc tccacggccg gccgacggca ggctggccag ggactcttcc cagacgccga
     3721 cgttgttggg ctgcggcgga taggggtgga ccccgaccac gcggacccgc cggccgtcgg
     3781 gcatcgtcag ctcggcccgc ggcatccgga aggcgaactg atccggccca ggcaggggcc
     3841 gcagcggcag ccgcgagtag aggcccgagc cggccgagcc ctgggcgact tcgagcagct
     3901 cgtgcggcag cagctcgccg agaccggccg cgcgcagttc gcgcgcgaac ctcggggtca
     3961 gctcctggac gctgagcagg tcggggtcgt ggcgctcgac cagggcgacg accgcctccg
     4021 cgtcggcgcg gccgcgcagg atgttggtcg agagcacgtc gagggtctcg cggccggcgg
     4081 cctcgacggt gccgtcgccg atcgcccgcg gcagcaccgc ggcggccagg cagaaggtgg
     4141 cgaaggcggc gacggcggcc gcggcccagt tctccagggc gacggccacg cccgcggtta
     4201 gcagcgccgc caccgcgacg taaggcgtga acggcatcgc cgtcgccagc gggaaaccgt
     4261 gatcgaggcc gagcacccgc aggaaggtcc agatcgcgac cgggatgacc gcagcccaga
     4321 ccagccagtt gcgcctcgca ctggcaaggg cggctgcggg gatggcgcca gcgcggccca
     4381 ggagtctcag ccgtccgggt agggctggat cgggacgccg tggtcggtct cccgcatccc
     4441 gaactcgt
//
LOCUS       DASRIG010000562         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3374378, whole genome shotgun sequence.
ACCESSION   DASRIG010000562 DASRIG010000000
VERSION     DASRIG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3374378"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2617)
                     /gene="lysS"
                     /locus_tag="VFB52_14955"
     CDS             complement(<1..2617)
                     /gene="lysS"
                     /locus_tag="VFB52_14955"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZO07686.1"
                     /translation="MSAEENGHPERAEFQEQTRAHRLAKLDGLRSRGIDPYPVRFDRT
                     STAAEIRTAFGEIAAGTDTGKVVKVAGRVLGERRHGGLDFADLQDETGKIQLIATRDA
                     VGDAELGGFSDLDLGDWIGVEGTVIASDKGELSVRVAKFELLSKSLRALPDVRHGVAD
                     PETRYRQRYLDLTLDEDSRRIFRIRSSIIATTRRVLTEKGFTEVETPVLLGQAGGAAA
                     KPFITHHNALNIDMTLRIALELPLKRLIVGGMNRVFEIGRVFRNEGLDTRHNPEFTLL
                     EAYQAFGDYHDMMDLTEAIIATSCQEAIGTTVVQINGQDVDLKPPWRRATMAELIKEN
                     AGVDMHPSMPVADARKIADERGVDYLDVWGSGKIMSEVYDDSSESKLIEPTFVMDHPR
                     EVSPLARAHRDDPELTERFELVVAGRELANAYSELNDPVDQAERFQTEADLQAGGDEE
                     GEPVDEDYVKALEYGLPPTGGLGIGMDRLVMLISGAEAIRDVILFPTLRPEGQEGADG
                     SAAVASDGAPSADAFAAPEAEEDDADLAAAERSAVAAAATLPPQAGRARSLRPLAWLS
                     VLVALFSLLPTATSLRFDIGHFSFIEGGGRSAGLIASVAIGLGIIAVARGLSRGKRRA
                     WAIAVILFAAAAVVHLIHGPDPISVLLSAGMLGALIWFRQDFRAHGDPGSLLQAALFV
                     PVYLLGVFAFTSITLFAERKHVTPDLTFWGNVETAYKGMVGLDGPYAYGREVFGDFFE
                     ITLIMLGVIGLLTFLFLLLRTFVQAEPPSAERRRRAEEIVRRWGDDTLDYFALRRDKS
                     YFFSSDGRSLIAYLYVRGTAMVAADPIGPPADTARTIDEFLTFCAERGWRVAFFAVRE
                     SDADLYKERGMHPIYL"
     gene            complement(2831..3679)
                     /locus_tag="VFB52_14960"
     CDS             complement(2831..3679)
                     /locus_tag="VFB52_14960"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZO07687.1"
                     /translation="MDIVLALAAALFFAFGTVLQQKAGLEKPAESEGAGLLVQMAKRP
                     VWLAGIAADALGFVCQAVALAIGRLAVVQPLLVATVVFALPLGAKFTGQKVRRTDVLA
                     ALLVLAALVAFLLIADPSGGIDDAPLGEWLVAIGICAAICVPIFLFRKRGTPGRRAAA
                     LGTATGILFALSAAFTKVVADQFEEGVFEIFVHWHLYALVVVGYASMTFNQLALNTGA
                     LAPAVATSLAFDPITSVVLGVTLLDESLHTSPLGIAGTVMALGAALAGMTVLARSQSQ
                     AEVAVT"
BASE COUNT          561 a         1317 c         1399 g          541 t
ORIGIN      
        1 cgaggtagat cgggtgcatg ccgcgctcct tgtagaggtc ggcgtcggac tcgcgcacgg
       61 cgaagaaagc gacccgccag ccgcgctcgg cgcagaaggt gaggaactcg tcgatcgtac
      121 gcgccgtgtc cgcgggcggc ccgatcgggt cggcggcgac catcgccgtc ccgcgcacgt
      181 agaggtaggc gatcagcgag cgcccgtcgc tggagaagaa gtagctcttg tcgcggcgca
      241 gggcgaagta gtcgagcgtg tcgtcgcccc agcggcggac gatctcctcg gccctgcggc
      301 ggcgctcggc gctcggcggc tcggcctgga cgaaggtccg cagcagcagg aacaggaagg
      361 tgagcagccc gatcacgccg agcatgatca gggtgatttc gaagaagtcg ccgaagacct
      421 cgcggccgta ggcgtagggc ccgtcgagcc cgaccatgcc cttgtaggcg gtttcgacgt
      481 tgccccagaa ggtcaggtcg ggggtgacgt gcttgcgctc ggcgaacagc gtgatcgagg
      541 tgaaggcgaa gacgccgagc aggtagaccg gcacgaacag cgccgcctgc agcagcgagc
      601 cggggtcgcc gtgggcgcgg aagtcctggc ggaaccagat cagcgcgccg agcatcccgg
      661 ccgagagcag gaccgagatc gggtccggcc cgtggatcag gtggacgacc gcggcggcgg
      721 cgaagaggat cacggcgatc gcccaggcgc gccgcttgcc gcggctgagc ccgcgcgcga
      781 cggcgatgat cccgagcccg atcgcgaccg aggcgatcag cccggccgag cggccgccgc
      841 cctcgatgaa gctgaagtgg ccgatgtcga agcgcagcga ggtcgcggtc ggcagcagcg
      901 agaagagcgc gaccaggacg ctgagccagg cgagcgggcg cagcgagcgg gcgcggccgg
      961 cctgcggcgg cagcgtcgcc gcggcggcaa ccgcggagcg ttcggccgcg gcgaggtcgg
     1021 cgtcgtcctc ctcggcctcg ggcgcggcga aggcgtcggc cgagggcgcc ccgtcggagg
     1081 cgaccgcggc ggagccgtcg gcgccctcct ggccctcggg gcgcagggtg gggaagagga
     1141 tcacgtcgcg aatcgcctcg gccccggaga tcagcatcac cagccggtcc atcccgattc
     1201 cgagcccgcc ggtcggcggc agcccgtact ccagcgcttt cacgtagtcc tcgtcgaccg
     1261 gttcgccctc ctcgtcgccg cccgcctgca ggtcggcctc ggtctggaac cgttcggcct
     1321 ggtcgaccgg gtcgttgagc tcgctgtagg cgttggcgag ctcgcggccg gcgacgacca
     1381 gctcgaagcg ctcggtcagt tccgggtcgt cgcggtgggc gcgggccagc ggcgagactt
     1441 cgcgcgggtg gtccatcacg aaggtcggtt cgatcagctt cgactcggag ctgtcgtcgt
     1501 agacctcgct catgattttc cccgagcccc agacgtcgag gtagtcgacc ccgcgttcgt
     1561 cggcgatctt gcgcgcgtcg gcgaccggca tcgaaggatg catgtccacg ccggcgttct
     1621 ctttgatcag ttcagccatc gtcgcccgcc gccagggcgg cttcaggtcg acgtcctggc
     1681 cgttgatctg gaccaccgtg gtcccgatcg cctcctggca ggaggtcgcg atgatcgcct
     1741 cggtcaggtc catcatgtcg tggtagtcgc cgaacgcctg gtaggcctcg agcagcgtga
     1801 actcggggtt gtggcgggtg tcgaggccct cgttgcggaa cacccggccg atctcgaaga
     1861 cccggttcat gccgccgacg atcagccgct tgagcggcag ctcgagcgcg atccgcaggg
     1921 tcatgtcgat gttgagcgcg ttgtggtggg tgatgaacgg tttcgccgcg gcgccgccgg
     1981 cctggccgag caggaccggc gtctcgacct cggtgaaccc tttttcggtc agcacccggc
     2041 gggtggtcgc gatgatcgag gagcggatcc ggaagatccg gcgcgagtcc tcgtcgaggg
     2101 tgaggtcgag gtagcgctgg cggtagcggg tctcggggtc ggcgaccccg tggcggacgt
     2161 cgggcagcgc ccgcaggctc ttcgagagca gctcgaactt ggcgacgcgc acgctcagct
     2221 cgcccttgtc ggaggcgatc accgtgccct cgaccccgat ccagtcgccg aggtcgaggt
     2281 cggagaagcc gccgagctcg gcgtccccga ccgcgtcgcg ggtcgcgatc agctggatct
     2341 tgccggtctc gtcctggagg tcggcgaagt cgaggccgcc gtggcggcgc tccccgagca
     2401 cccgcccggc gaccttcacg actttgccgg tgtcggtgcc ggcggcgatc tcgccgaagg
     2461 cggtgcgaat ctcggccgcg gtcgaggtgc ggtcgaagcg caccggatag gggtcgatcc
     2521 cgcgcgagcg caggccgtcc agcttcgcca ggcggtgcgc ccgggtctgc tcctggaact
     2581 cggcgcgttc ggggtggccg ttctcctcag cgctcatccc gcccgatcct agacgccact
     2641 caggcgggtg ccggggcgga ctcgacgggt ttcggaccct cgggtggccg tcacctgatc
     2701 cccacgcgcg cctggcggcc ccggacacac ttcgcggggc gtcgtcctca gtcggccaaa
     2761 tggctctgcc attcggcctc cctgcgtcct atccccgctc ggtccggccc tcgccagtcg
     2821 cacgcgcgga tcaggtgacg gccacctcgg cctgcgattg cgaacgcgcc agcaccgtca
     2881 ttccggccag cgcggctccc agcgccatca cggtcccggc gatccccagc ggcgaggtgt
     2941 gcagcgactc gtcgagcagg gtgacgccga ggacgacgct ggtgatcggg tcgaaggcga
     3001 ggctggtggc gaccgccggc gccagcgccc cggtgttcag ggccagctgg ttgaaggtca
     3061 tcgaggcgta gccgacgacg accagcgcat agaggtgcca gtggacgaag atctcgaaga
     3121 ccccttcctc gaactggtcg gcgacgacct tggtgaaggc ggccgagagc gcgaagagga
     3181 tgccggtcgc ggtccccagg gcagccgccc gccggcccgg cgtgccgcgc ttgcggaaga
     3241 ggaagatcgg cacgcagatc gccgcgcaga tgccgatcgc gaccagccac tcgccgagcg
     3301 gcgcgtcgtc gatgcctccc gacgggtcgg cgatcagcag gaaggcgacc agcgccgcga
     3361 gcacgagcag cgccgccagc acgtcggtcc ggcgcacctt ctgcccggtg aacttggcgc
     3421 cgagcggcag cgcgaagacc acggtcgcga ccagcagcgg ctgcaccacc gcgagccgcc
     3481 cgatcgccag cgccaccgcc tggcagacga acccgagcgc gtcggcggcg atcccggcca
     3541 gccagaccgg ccgcttcgcc atctgcacga gcagccccgc cccttcgctc tcggccggct
     3601 tttccagccc ggccttctgc tgaagcacgg tgccgaaggc aaagaacaga gccgccgcga
     3661 gggccaagac gatgtccacg cccggaccct acccagctat ggcgtcgccg tagcctccgc
     3721 ggcgtcggcc gccgcgcccc ctttgacccc ggcgctgacg ccggcgtcgc tggagaggcg
     3781 gtgggcgagg tagaccggga tgatcgagat caggatca
//
LOCUS       DASRIG010000563         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2341789, whole genome shotgun sequence.
ACCESSION   DASRIG010000563 DASRIG010000000
VERSION     DASRIG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341789"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFB52_14965"
     CDS             <1..926
                     /locus_tag="VFB52_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZO07688.1"
                     /translation="AIRRFLDDEGFIEVETPVLQPIYGGALARPFVTHFNALDRTMYL
                     RVATELYLKRLIVGGLERVYEIGKDFRNEGLSPKHNPEFTMLEWYEAYADYEKTAHDL
                     EMMIAETAERVLGTTVVKRGEVEIDFKPPWRRITLREAILERSGIDIAEHPEREALAR
                     AIGGEVDPKAGWGKLVDGLLSRDVEPGLIQPTFLLDYPTELSPFAKAHREKPDLVERW
                     EAFAGGVEIANSFSELNDPDEQKRRFEQQAAEIARGDEEAQPYDEIYIEALEQGMPPT
                     GGVGLGIDRLVMMLTGAPSIREVLLFPAMKS"
     gene            complement(940..2223)
                     /locus_tag="VFB52_14970"
     CDS             complement(940..2223)
                     /locus_tag="VFB52_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07689.1"
                     /translation="MSSSPLAEFAESHGLTYSESAVLPAQGGTLTQGSGKVEGAATGT
                     LPGGIEGTLARFAYVYKSTDSDGKTTEERRPFTLVVTEIPESIGFVPYLGFSGRASRL
                     SARAGGEDMAPIAMKHVEALKGASAYAYKGVSEHWLAQLLSPAMVQWLGRCDDDFGFE
                     LASGVLIAGRGGYLEDALKLEALCTDAAHLAGAIREESLEEVGTGGESEAARDVRFAD
                     PQTEAALRDVAVPSPTDVTAARAEFASHLRGSGMTLFRALRSGVLWGLIVNVPAVAIP
                     IILISEGLYLLLAIIEAALILLIATFSFRSRVRSGAEKFAAEAFYRAYAADRELSMVE
                     PLGFLANHAEAKLGFKPDRVFTGVLPGGMNGALALAGNGSKRSDRIAAIAGPRGPVAE
                     AELQAEEPGLSATVLDDYARRLSAEVAEDIATRPA"
     gene            2436..>2982
                     /locus_tag="VFB52_14975"
     CDS             2436..>2982
                     /locus_tag="VFB52_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZO07690.1"
                     /translation="MFERFTERARQVVVLAQEEARTLKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVERVRSQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSDKIRNEVIRMLSGPGGRRSGSGGSSGQGE
                     GKKSSKLLDQFGRNLTRLAEEG"
BASE COUNT          486 a          986 c         1070 g          440 t
ORIGIN      
        1 ccgcgatccg ccgcttcctc gacgacgagg ggttcatcga ggtcgagacc ccggtcctgc
       61 agccgatcta cggcggcgcg ctggcgaggc cgttcgtgac ccacttcaac gcgctcgacc
      121 gcacgatgta cctgcgggtc gcgaccgagc tgtacctgaa gcggctgatc gtgggcgggc
      181 tcgagcgcgt gtacgagatc ggcaaggact tccgcaacga gggtctgtcc ccgaagcaca
      241 accccgagtt cacgatgctg gagtggtacg aggcctacgc ggactacgag aagaccgccc
      301 acgacctcga gatgatgatc gccgagaccg ccgagcgggt gctggggacg acggtcgtca
      361 aacgcggcga ggtcgagatc gacttcaagc cgccctggcg ccggatcacc ctgcgcgagg
      421 cgatcctcga gcgctccggg atcgacatcg ccgagcaccc cgagcgcgag gcgctggccc
      481 gggcgatcgg cggcgaggtc gacccgaagg cgggttgggg caagctggtc gacggcctgc
      541 tctcgcgcga cgtcgagccg gggctgatcc agccgacttt cctgctcgac tacccgaccg
      601 agctctcgcc cttcgccaag gcccaccgcg agaagccgga cctggtcgag cgctgggagg
      661 cgttcgcggg cggggtcgag atcgccaact ccttcagcga gctgaacgac ccggacgagc
      721 agaagcggcg cttcgaacag caggccgccg agatcgcccg cggcgacgag gaggcccagc
      781 cctacgacga gatctacatc gaagcgcttg aacagggcat gccgccgacc ggcggcgtcg
      841 gcctcggcat cgaccgcctg gtgatgatgc tcacgggcgc cccctcgatc cgcgaggtcc
      901 tgctgtttcc cgcgatgaag tcctgagcga cgcggccctt caggccggtc gggtggcgat
      961 gtcttcggcg acttcggcgc tgagccggcg ggcgtagtcg tcgaggacgg ttgcagagag
     1021 gccgggttcc tcggcctgca gctccgcttc ggcgaccggg ccgcgggggc cggcgatcgc
     1081 ggcgatccgg tcggagcgct tgctgccgtt gccggccagg gcgagggcgc cgttcatgcc
     1141 gccgggaagg accccggtga agacgcggtc gggtttgaag ccgagcttcg cttccgcgtg
     1201 gttggcgaga aagccgagcg gctcgaccat cgacagttca cgatcggcgg cgtaggcccg
     1261 gtagaaggcc tcggcggcga acttctcggc gccgctgcgg acgcggctgc ggaaggagaa
     1321 agtggcgatc aggaggatta gggccgcctc gatgatcgcc agcagcaggt agaggccttc
     1381 ggagatcagg atgatcggga tcgcgaccgc ggggacgttg acgatcaggc cccagaggac
     1441 gccggagcgc agcgcgcgga agagcgtcat cccggaaccg cgcaggtgag aggcgaactc
     1501 ggcgcgggcg gcggtgacgt cggttggcga gggcacggcg acgtcgcgca gcgccgcctc
     1561 ggtctgcggg tcggcgaagc ggacgtcgcg cgccgcttcg gactcgccgc cggtcccgac
     1621 ctcctccaac gactcttcgc ggatcgcgcc ggccaggtgg gcggcgtcgg tgcagagcgc
     1681 ctccagcttc agcgcgtcct cgaggtagcc gccgcggccg gcgatcagca cgccgctcgc
     1741 cagttcgaag ccgaagtcgt cgtcgcagcg gcccagccac tggaccatcg ccggcgagag
     1801 cagctgcgcc agccagtgct cggagacgcc cttgtaggcg taggcgctcg cccccttcag
     1861 cgcctcgacg tgcttcatcg cgatcggcgc catgtcttcg ccgccggctc gcgcggagag
     1921 cctgctggcc cggccggaga agccgaggta ggggacgaag ccgatgctct cggggatctc
     1981 ggtgacgacg agggtgaacg gacgccgctc ttcggtcgtc ttgccgtcgg agtcggtcga
     2041 cttgtagacg taggcgaagc gggccagggt gccctcgatt ccgcccggca acgtccccgt
     2101 cgcggcccct tcgaccttgc cgctgccctg ggtcagggtg ccgccctggg cgggcagaac
     2161 ggcgctttcg gagtaggtga ggccgtggct ctcggcaaac tcggcgagcg gggaagagga
     2221 cacgcaggga tcctaatcgc gccttaatcg ctgctttggg ccgaatttgg gtctactcct
     2281 gtacgaaacc ccgctcttag cgcacgagcg gcgcaccgag ccggactgac gcgctcggta
     2341 gagttaaggg acacaagggc accttccgat atcgatgagg tgcctaccgg tagtaactcg
     2401 ggccacccgg gttggaccaa gaagaggttt gcactttgtt cgagcgattc actgagcgag
     2461 cccgtcaggt cgtcgtccta gcgcaggagg aggcccggac tctcaagcac aactacatcg
     2521 gcaccgagca catcctgctt ggtctccttc gcgaggagga ggggctggcc gcgcgcgtgc
     2581 tcgagtccct ggacatcacc gtcgaaaggg tgcgctcgca ggtcgttcgg atcgtcggct
     2641 ccggcgagga ggtgacctcc ggccagatcc cgttcacccc gcgggccaag aaggtgctcg
     2701 agctggccct gcgcgaggcg ctctcgctcg gccacaacta catcggcacc gagcacatcc
     2761 tgctcggcct ggtgcgtgag aacgagggcg tcgcagcccg catcctgctc gacttcgacg
     2821 ccgactcgga caagatccgc aacgaggtga tccggatgct ctccggcccc ggcggccggc
     2881 gcagcggcag cggtggcagc agcggacagg gcgagggcaa aaagtcttcg aaactgctcg
     2941 accagttcgg gcgcaacctc acgcgcctcg ccgaggaggg ca
//
LOCUS       DASRIG010000564         8736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3378152, whole genome shotgun sequence.
ACCESSION   DASRIG010000564 DASRIG010000000
VERSION     DASRIG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378152"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..480
                     /locus_tag="VFB52_14980"
     CDS             43..480
                     /locus_tag="VFB52_14980"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZO07691.1"
                     /translation="MADETLQMERTFQAPAQAVFDAWTSEEVTRRWFRGREVFETPFA
                     SVDLRVGGAVRVVMRNPEDGTEYGGGGHYTEIDPPNRLAFTWVWDDEAERETQIEIEF
                     EEADGATTVRFTHSNLRDQEAVHSHEDGWGTCFDLLERALQSS"
     gene            complement(563..2086)
                     /gene="lysS"
                     /locus_tag="VFB52_14985"
     CDS             complement(563..2086)
                     /gene="lysS"
                     /locus_tag="VFB52_14985"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZO07692.1"
                     /translation="MSEAESPAQPVVDPTGDRSGAASEAQRRAKVERLREKGINPYPH
                     NFFGRTHVEEIVAAHDPAELGEGEHEEFSYRVMGRVTGKRGHGKVVFLDVRDITGSIQ
                     AVAKRDKLGEEAFERIEDLDIGDVIGVEGVIYVTKRGQLAIGVGECTLLAKAVLDPPD
                     LFHGISDPELRYRQRELDLMANEDSREIFKKRSKLTLAIRKHMDERGWVELETPVLQR
                     LTGGAAARPFVTHHNALDRDLFLRTATELMLKRAIVGGFEDVYEFGKFFRNEGMSPQH
                     NPEFTMLEMFVGGSDWTGVMEFIEVLVASVVEEVMGTTKVERDGETIDFAAPWPRIVL
                     RDALLEETGVDIYKASRDELAEIAGDDAEENDDWAGLVDTLQGKLVEPKLIQPTYITV
                     LPIDIWPLVKVHPDDPKVCEAFDGIVAGMEIVGGGTDVNDPVEQRERFIGQRERQEAG
                     AEEDPHPHDEEFVKALEYGMPPASGCGLGIDRLLMIMTGAKTLRDVIIYPAMREQSS"
     gene            complement(2103..2900)
                     /locus_tag="VFB52_14990"
     CDS             complement(2103..2900)
                     /locus_tag="VFB52_14990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HZO07693.1"
                     /translation="MSAVLDDAAERIAAAGVENARADAEVLVADALGVAPGELSVESA
                     GEIDTGVAAAIEERVARRVDREPMAYILGRAPFRNLEIYVDERVLWPRRETELLVEVG
                     AKLPEGARVHEVGTGSGAIALALISERPDLKVTASDLSPEAVEAARENAERLGLDFDV
                     TVATGIPDGLEDVDLVIANLPYVTDETIFERSPEIQREPRIAVTGDCGADGLGVIRGL
                     IAETPSGWQLAMEHDTHHGPAMRELLRDATTLQDYEGDERVTVGFAP"
     gene            complement(3035..3820)
                     /locus_tag="VFB52_14995"
     CDS             complement(3035..3820)
                     /locus_tag="VFB52_14995"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZO07694.1"
                     /translation="MYPEIVIGPVTLQTFGLCFALGFLAAGALIWKRFKETGHPTDWA
                     YEMGFAALVGGVVGSRLYYIAQNYSDVKDDLLGNLFSGGGLVWYGGAIGGAIAVLLWA
                     WWRGFLGLALLDVAAPALAIGYAIGRCGCQLSGDGDYGKAWDGPWAMSYPDGTVPTDE
                     AVHPTPVYESLAMGLCAWLLWQLRDRVRPGMLFAIYLVYAGLERFLIEFLRRNDPGTL
                     GLTTAQIESLTMVLGGAIWIAIVARRHGGIGLPTANETAPRFT"
     gene            complement(3821..4708)
                     /gene="argF"
                     /locus_tag="VFB52_15000"
     CDS             complement(3821..4708)
                     /gene="argF"
                     /locus_tag="VFB52_15000"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZO07695.1"
                     /translation="MTGETRNFLTGEELGAFELGKLLDRAAELKAGRRDGVGADSLAG
                     RSVALVFEKPSTRTRISFEVGVAELGAHPVVLRGDEMQIARGESIGDTARVLSRYVDA
                     IVIRSGSHETVAGLAAEAEVPVINGLTPLHHPCQALADLLTMRERFGAIEGRRLAYVG
                     DGNNVARSLAILGELAGVEVVVAAPPGYQLEEGHEAKLTGDPRAAAAGADALYTDVWI
                     SMGDEAEAERRRADLAPFRLDGDLLAAANDDAIVLHCLPAHPGEEISADVLYGERSAV
                     WDQAENRLHAQKALLEMLI"
     gene            4727..5566
                     /locus_tag="VFB52_15005"
     CDS             4727..5566
                     /locus_tag="VFB52_15005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZO07696.1"
                     /translation="MEFTALAWNLFHGRDFPPGSELRTWRSRLLRFDERSETHVQVNR
                     DLGKEFATLLATTAWDVALLQESPPRFAAALARACGAEQHLVLTSRNSFGALRALLAR
                     QNPDLMASGEGGSNLTLVRVPGKLGGIAERRELTIHEGEPERRAMAFTRTASGVCVAN
                     LHATNDIPEMAIEDVLRAARAADRWAGDSPLVFGGDLNLRPEENPEVFAQLEAELGLA
                     APTAPRAIDHVLCRGLEVVEKPSAWPPERRELPLEGRALRLSDHAPVEAKFHIPESLM
                     ARN"
     gene            5614..6069
                     /locus_tag="VFB52_15010"
     CDS             5614..6069
                     /locus_tag="VFB52_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07697.1"
                     /translation="MAEKKAAGKAAKAKPKKAKAKATKAKKAPAKKATKKAAPKKAAS
                     KASSSKATTGLDKSVAQFRESLEQSVTLSRDRIQEVVDDAVQRGRMTRGDAEKLLSDL
                     VKRGRKQTDTLLGELERLVKQARKEVGGHAEPVRKKATQAARRARKKLG"
     gene            6074..7459
                     /gene="rlmD"
                     /locus_tag="VFB52_15015"
     CDS             6074..7459
                     /gene="rlmD"
                     /locus_tag="VFB52_15015"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZO07698.1"
                     /translation="MTQATGDKSAARRPKRGELLEVEVESLAFGGRGVARTEGLVVFV
                     AGGLPGDKVRAEVTKAKKRFAEARVVELLSPSAERVADLCVHDGEPCPGAPWQGLDYE
                     AQLSHKSAQVDEALRRIGELDGFELEPIVAAEQQWRYRNKLEYSFGEDDEGEPTLGFH
                     ARGRWDLIVNVDDCHLASAVGNEARNAVREWARVESVPTYDGRARRGVLRNLAVREGR
                     RTGQIQTRLVTSPARFPRPPVDLHTQIEGDSGGTDGPTGVLGEERLREQLCGLDLEMS
                     YAAFFQTNTEMAERLYGVAAEFADLKGTERVFDLYCGIGTIGLTLARQAGEVWGLEIV
                     PEAIADAKLNAERNQIENANFLAANARTGVRPLIEKAGKADVVVIDPPRAGLSQKIVR
                     RVLECEAQKIVYVSCNPTTLAPNAAQLVEAGYRLSRVRPVDMFPQTPHIECVALFEKT
                     AASTPAKGADA"
     gene            7459..8247
                     /locus_tag="VFB52_15020"
     CDS             7459..8247
                     /locus_tag="VFB52_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZO07699.1"
                     /translation="MSGRAFGVAAGLDPEVARPLAARCEELGYHSVWSNDHPGAKGLD
                     TLADFAAAAPGIDLGVGVIAIDRTPPETIAADVERTGLDPSRLWLGVGAGFNKKPLTA
                     MREVLPELRERLPEVRIVLASMGPKMCALAGAEYDGAFFNWMTPEYAAGARVKVEAGA
                     AEAGREAPPVFGYVRTAVGEDAAARLAKEESFYRDLHKGYRDHFDRLGEPEGTVGVAA
                     ADPAAAQIGLAAYTALDTAVVRGLASAKVESMVAVAEAAAPPHV"
     gene            8378..>8736
                     /locus_tag="VFB52_15025"
     CDS             8378..>8736
                     /locus_tag="VFB52_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HZO07700.1"
                     /translation="MQAPLYLGLIVAQGLCVYRFFVELWHLISHAGALHETQVMLIVL
                     GLIDVVMIANLLIMVIIGGYETFVSRLGKDEHPDRPELLGHVNASTMKIKLAMALIGI
                     SSIHLLKTFIGAHSIEDR"
BASE COUNT         1380 a         3113 c         2981 g         1262 t
ORIGIN      
        1 ctggcttgcg ctgcgctggc gcgaccgcga ggatcgggac tgatggccga cgagacgctg
       61 cagatggaac gcaccttcca ggcccccgcc caggcggtct tcgacgcctg gaccagcgag
      121 gaggtgacgc gccgctggtt ccgcgggcgc gaagtcttcg agaccccgtt cgcctccgtc
      181 gacctgcgcg tcggcggagc cgtgcgcgtg gtcatgcgca atcccgagga cggcaccgag
      241 tacgggggtg gcggccacta caccgagatc gacccgccca accggctcgc cttcacctgg
      301 gtctgggacg acgaggccga gcgcgagacc cagatcgaga tcgagttcga ggaggccgac
      361 ggcgctacca cggtccgctt cacgcactcg aacctgcgcg accaggaggc ggtccacagc
      421 cacgaagacg gctggggaac ctgcttcgac ttgctcgagc gggcactaca gagttcgtag
      481 gaccaagcga gggcgtgccg gcggcgaagc ggcagtgctc tgcacgacgc gaagccgacg
      541 gtgcggcctc gcgcagtgtg tcctacgagc tctgctcgcg catggcgggg tagatgatca
      601 cgtcgcggag cgtcttcgcc ccggtcatga tcatcagcag ccggtcgatc ccgaggccgc
      661 agccgctggc cggcggcatc ccgtattcga gcgctttgac gaactcttcg tcgtgcgggt
      721 gcggatcctc ctccgccccg gcttcctggc gttcgcgctg gccgatgaag cgttcgcgct
      781 gctcgaccgg gtcgttgacg tcggtgccgc cgccgacgat ctccatcccg gcgacgatcc
      841 cgtcgaaggc ctcgcagacc ttggggtcgt cggggtggac cttgaccagc ggccagatgt
      901 cgatcggcag cacggtgatg taggtcggct ggatcagctt cggctcgacc agcttgccct
      961 gcagggtgtc gacgaggccg gcccagtcgt cgttctcttc ggcgtcgtca ccggcgatct
     1021 cggccagctc gtcgcggctc gccttgtaga tgtcgacgcc ggtctcctcg agcagcgcgt
     1081 cgcgcaggac gatccgcggc cagggcgcgg cgaagtcgat cgtctcgccg tcgcgctcga
     1141 ccttcgtcgt ccccatcacc tcctcgacca cgctggcgac gagcacctcg atgaactcca
     1201 tcacgccggt ccagtcggac ccgccgacga acatctcgag catcgtgaac tcggggttgt
     1261 gctgcggcga catcccctcg ttgcggaaga acttgccgaa ctcgtagacg tcctcgaagc
     1321 cgccgacgat cgcccgcttc agcatcagct cggtcgcggt gcgcaggaag aggtcgcggt
     1381 cgagcgcgtt gtggtgggtg acgaaggggc gcgccgcggc gccaccggtg agccgctgca
     1441 ggaccggcgt ttccagctcg acccagccgc gctcgtccat gtgcttgcgg atcgcgaggg
     1501 tcagtttcga gcgcttcttg aagatctccc gtgagtcctc gttggccatc aggtcgagtt
     1561 cgcgctggcg gtagcgcagc tccgggtcgg agatcccgtg gaagaggtcg ggcgggtcga
     1621 gcaccgcctt ggccagcagc gtgcactcgc cgacgccgat cgccagctgc ccgcgtttgg
     1681 tcacgtagat cacaccctcg accccgatca cgtcgccgat gtcgaggtct tcgatccgct
     1741 cgaaggcctc ctcgccgagc ttgtcgcgtt tggcgaccgc ctggatcgag ccggtgatgt
     1801 cgcggacgtc gaggaagacg actttgccgt gaccgcgctt gccagtcacc cggcccatca
     1861 cccggtagct gaactcctcg tgttcgccct cccccagctc ggccggatcg tgggcggcga
     1921 cgatctcctc gacgtgggtg cgaccgaaga agttgtgcgg gtaggggttg atccccttct
     1981 cgcgcaggcg ctcgaccttg gcgcggcgct gcgcctcgct cgccgccccg gagcgatcgc
     2041 cggtcggatc aacgaccggc tgcgccggcg actccgcctc actcatcgac tacagccgtc
     2101 cgtcatggag cgaagccgac agtcacccgc tcgtcccctt catagtcctg aagggtagtg
     2161 gcgtcgcgca agagctcgcg catcgccggg ccgtggtgag tgtcgtgctc catcgccagc
     2221 tgccagccgg agggcgtttc cgcgatcagc ccgcggatca cgccgaggcc atcggcgccg
     2281 cagtcgccgg tgacggcgat ccgcggctca cgctggatct cgggcgagcg ctcgaagatc
     2341 gtctcgtcgg tgacgtaggg caggttggcg atcaccaggt cgacgtcctc gaggccgtcg
     2401 gggatcccgg tggcgacggt gacgtcgaag tcgaggccga gccgctcggc gttctcgcgg
     2461 gcggcttcga ccgcctccgg ggagaggtcc gaggcggtga ccttgaggtc gggacgctcg
     2521 gagatcagcg ccagcgcgat cgcgccggaa ccggtgccga cctcgtggac gcgggcgccc
     2581 tcgggaagtt tggcgccgac ctcaaccagc agctcggtct cgcggcgcgg ccagaggacg
     2641 cgctcgtcga cgtagatctc gaggttgcgg aagggcgcgc ggccaaggat gtaggccatc
     2701 ggctcgcggt cgacgcggcg cgcgacccgc tcctcgatcg ccgcggcgac cccggtgtcg
     2761 atctcgccgg cgctctcgac cgagagctcg cccggcgcga cgccaagcgc gtcggcgacc
     2821 aggacttcgg catcggcgcg ggcgttctcg acccccgctg cggcaatccg ctcggcggcg
     2881 tcatcgagca ccgcactcac ggtgcgggcc gaaggtccct cgctctgaat gtcaattgcc
     2941 gccatctttt agattgccct ttccgaattg ctccaaagat cttgcgcaag actaacaccg
     3001 attctgtgtc agtccgaacg cagaatcgtt gcattcaggt gaaccgcggt gcagtctcgt
     3061 tcgccgtggg caggccgatg cctccgtggc gacgggccac gatagcgatc cagatcgctc
     3121 cgccgagcac catcgtgagg ctctcgatct gcgccgtggt cagccccagc gtgcccgggt
     3181 cgttgcggcg taagaactcg atcaggaagc gctcgagccc ggcgtagacc aggtagatcg
     3241 cgaacagcat ccccggccgc acgcgatcgc gcagctgcca gagcagccag gcgcagagcc
     3301 ccatcgccag cgactcgtag acgggagtcg ggtgcacggc ttcgtcggtc ggcacggtcc
     3361 cgtccgggta ggacatcgcc cagggcccgt cccaggcctt gccgtagtcg ccgtcgccgg
     3421 agagctggca gccgcagcgg ccgatcgcgt agccgatcgc cagggccggc gccgccacgt
     3481 cgagcagcgc gaggccgagg aagccgcgcc accaggccca gagcaggacc gcgatcgcgc
     3541 cgccgatcgc gccgccgtac cagaccaggc cgccgccgga gaagaggttg ccgagcaggt
     3601 cgtccttgac gtcactgtag ttctgggcga tgtagtagag ccgcgagccg acgacgccgc
     3661 cgaccagcgc cgcgaagccc atctcgtacg cccagtcggt cgggtgccca gtctccttga
     3721 agcgcttcca gatcagcgcg ccggcggcga ggaagccgag cgcgaagcag agaccgaagg
     3781 tctgcagcgt gaccggcccg atcacgatct cggggtacat ctagatcagc atctccagca
     3841 gcgccttctg cgcgtgcagc cggttctcgg cctggtccca gacggcggag cgctcgccgt
     3901 agaggacgtc ggcgctgatc tcttcgccgg ggtgggcggg caggcaatga agcacgatcg
     3961 catcgtcgtt ggccgcggcg agcaggtcgc cgtcgagccg gaacggcgcc aggtcggcgc
     4021 ggcggcgctc ggcctcggcc tcgtcgccca tgctgatcca gacgtccgta tatagagcgt
     4081 cggccccggc cgccgccgcg cgcgggtcgc cggtcagctt cgcttcgtgc ccctcctcca
     4141 actggtagcc gggaggcgcc gcgacgacca cctcgacccc ggccagctcg ccgaggatcg
     4201 ccagcgagcg ggcgacgttg ttgccgtcgc cgacgtaggc cagccgccgc ccctcgatcg
     4261 cgccgaagcg ctcccgcatc gtcagcaggt cggcgagcgc ctggcagggg tggtgcagcg
     4321 gggtcagccc gttgatcacc ggcacctcgg cctcggccgc cagcccggcg acggtctcgt
     4381 gcgagcccga gcggatcacg atcgcgtcga cgtagcggga gagcacccgc gcggtgtcgc
     4441 cgatcgactc gccgcgggcg atctgcattt cgtcgccgcg caggacgacc gggtgggcgc
     4501 cgagctcggc gaccccgacc tcgaaggaga tccgcgtccg ggtcgagggc ttttcgaaga
     4561 cgagggcgac gctgcgcccg gccagcgagt cggcgccgac gccgtcgcga cggccggctt
     4621 tcagctcagc ggcgcggtcg agcagcttgc cgagctcgaa cgcgccgagc tcctcgccgg
     4681 tgaggaaatt gcgagtttcg ccggtcaagc cgcggatact acggcggtgg agttcacggc
     4741 cctcgcctgg aacctgttcc acgggcggga tttcccgccc ggttcggagc tgcgcacctg
     4801 gcgctcgcgc ctgctgcgct tcgacgagcg cagcgagacc cacgtccagg tcaaccgcga
     4861 cctcggcaag gagttcgcga cactgctcgc gaccacggcc tgggacgtcg ccctgctgca
     4921 ggagtccccg ccccgcttcg ccgccgcgct ggcccgcgcc tgcggggccg agcagcacct
     4981 ggtgctgacc tcgcgcaact ccttcggagc gctgcgggcg ctgctggcgc ggcagaaccc
     5041 ggacctgatg gcctccggcg agggcggttc caacctgacc ctggtccgcg tccccggcaa
     5101 gctcggcggg atcgccgagc ggcgcgagct gacgatccac gaaggcgagc cggagcgccg
     5161 ggcgatggcc ttcacccgca ccgcctccgg cgtctgcgtc gccaacctgc acgcgaccaa
     5221 cgacatcccg gagatggcga tcgaggacgt gctgcgcgcg gcgcgggcag ccgaccgctg
     5281 ggcgggcgac tcaccactcg tcttcggcgg cgacctcaac ctgcgcccgg aggagaaccc
     5341 ggaggtcttc gcgcagctgg aggcggagct gggcctggcg gcgccgaccg cgccgcgggc
     5401 gatcgaccac gtcctctgcc gcggcctcga ggtggtcgag aaaccgtcgg cctggccgcc
     5461 ggagcggcgc gagctgccgc tcgaagggcg tgccctgcgg ctctccgacc acgcaccggt
     5521 cgaggccaag ttccacattc ccgagtcact gatggcacgg aattgagata gtcgggtaca
     5581 cccggggcaa acaccagtct ggaggcagtc gagatggcgg agaaaaaagc agcgggcaaa
     5641 gcggcaaaag cgaaaccgaa aaaagcgaaa gccaaagcga ccaaagcgaa aaaagccccg
     5701 gccaaaaaag ccaccaaaaa agcggctccg aaaaaagcgg cttcaaaagc ttcgagcagc
     5761 aaagcgacga ccggcctcga caagagcgtc gcccagttcc gcgagagcct cgagcagagc
     5821 gtcacgctca gccgggaccg catccaggag gtcgtcgacg acgccgtcca gcgcggccgg
     5881 atgacccgcg gcgacgccga gaagctgctc agcgacctgg tcaaacgcgg tcgcaaacag
     5941 accgacaccc tgctcgggga gctcgagcgg ctggtcaagc aggcgcgcaa agaggtcggc
     6001 ggccacgccg agccggtccg caaaaaagcg acccaggcgg cgcgccgcgc acgcaaaaag
     6061 ctcggctgac ccggtgacgc aggcgaccgg cgacaagtcg gcggctcgcc gtccgaaacg
     6121 gggcgagctg ctcgaggtag aggttgaatc gctcgccttc ggcgggcgcg gcgtcgcccg
     6181 taccgaaggg ctggtcgtgt tcgttgccgg cggactgccc ggcgacaagg tccgcgccga
     6241 agtcacaaaa gcgaagaagc ggttcgccga ggcgcgcgtg gtcgagctgc tctcgcccag
     6301 cgccgagcgc gtcgccgacc tctgcgtgca cgacggcgag ccctgccccg gcgccccctg
     6361 gcaggggctc gactacgagg cccagctcag ccacaagtcc gcccaggtcg acgaggcgct
     6421 gcgccggatc ggtgagctcg acggcttcga gctggagccg atcgtcgccg ccgagcagca
     6481 gtggcgctac cgcaacaagc tcgagtactc cttcggcgag gacgacgagg gcgagccgac
     6541 gctcggcttc cacgcccgcg gccgctggga cctgatcgtc aacgtcgacg actgccacct
     6601 cgcttccgcc gtcggcaacg aggcccgcaa cgcggtccgc gagtgggccc gggtcgagtc
     6661 ggtccccacc tacgacggtc gcgcccgccg cggcgtgctg cgcaacctcg ccgtgcgcga
     6721 gggccggcgc accggccaga tccagacccg gctggtgacc agcccggccc gcttcccgcg
     6781 cccgccggtc gacctccaca cccagattga gggcgacagc ggcggcaccg acggcccgac
     6841 cggggtgctc ggcgaggagc ggctgcgcga gcagctctgc ggcctcgacc tggagatgtc
     6901 ctacgccgcc ttcttccaga ccaacaccga gatggccgag cgcctctacg gcgtcgccgc
     6961 cgagttcgcc gacctcaagg gcacggagcg cgtcttcgac ctctactgcg ggatcggcac
     7021 gatcgggttg accctggcac gccaggccgg cgaggtctgg gggctggaga tcgtccccga
     7081 ggcgatcgcc gacgccaaac tcaacgccga acgcaaccag atcgagaacg ccaacttcct
     7141 cgccgccaac gcccgcaccg gcgtccgccc gctgatcgag aaggccggca aagccgacgt
     7201 cgtcgtgatc gacccgccgc gcgccgggct ctcccagaaa atcgtccgcc gcgtgctcga
     7261 gtgcgaggcg cagaagatcg tctacgtctc ctgcaacccg accacgctgg cgccgaacgc
     7321 ggcccagctg gtcgaggccg gctataggct gagccgggtg aggccggtcg acatgttccc
     7381 gcagaccccg cacatcgagt gcgtggcgct gttcgagaaa accgccgcca gcacgccggc
     7441 caaaggagcc gacgcctgat gtccggacgc gccttcggag tagcggccgg gctcgaccca
     7501 gaggtggcgc gcccgctcgc cgcccgctgc gaagagctcg gctaccactc ggtctggtcc
     7561 aacgatcacc cgggcgccaa aggcctcgac accctggccg atttcgccgc cgcggcgccg
     7621 gggatcgacc tcggggtcgg cgtgatcgcg atcgaccgca cgccgccgga gacgatcgcg
     7681 gcggacgtcg agcgcaccgg cctcgacccc tcccgcctct ggctcggcgt cggcgccggc
     7741 ttcaacaaga agccgctgac cgcgatgcgc gaggtgctgc cggagctgcg cgagcggctg
     7801 cccgaggtgc ggatcgtgct cgcctcgatg gggccgaaga tgtgcgcgct cgccggcgcc
     7861 gagtacgacg gcgccttctt caactggatg accccggagt acgcggccgg ggcgcgggtc
     7921 aaagtcgagg ccggcgccgc cgaggccggc cgcgaggcgc cgccggtatt cggctacgtg
     7981 cgcaccgccg tcggcgagga cgccgccgcc cgcctggcca aggaggagtc cttctaccgc
     8041 gacctgcaca agggctaccg cgaccacttc gaccgcctcg gcgagcccga gggcacggtc
     8101 ggggtcgccg ccgccgaccc cgcggccgcg cagatcggcc tcgccgccta caccgcgctc
     8161 gacaccgccg tcgtccgcgg tctcgccagc gccaaggtcg agtcgatggt cgccgtcgcc
     8221 gaggcggccg ccccgccgca cgtctgacgc ggttccttag gggcgcctaa gcctttttgg
     8281 ctagagtccc ggccccatct cggcctcacc caaacagccc cccatagacc gaccgctcgc
     8341 ccgctacgtc ggcacgacga tcttcctcag ccgctggctg caggcgccgc tctacctcgg
     8401 tctcatcgtc gcccagggtc tgtgcgtcta ccgcttcttc gtcgagctgt ggcacctcat
     8461 cagccatgcc ggcgccctgc acgagaccca ggtcatgctc atcgtcctcg ggctcatcga
     8521 cgtggtcatg atcgccaacc tgctgatcat ggtgatcatc ggtgggtacg agacttttgt
     8581 gtccagattg ggaaaagacg agcatccgga ccggccggaa ttgctcgggc atgtcaacgc
     8641 cagcacaatg aaaatcaagc ttgcaatggc acttatcggg atctcgtcaa tccacttgct
     8701 gaagaccttt atcggcgctc attcaatcga ggatcg
//
LOCUS       DASRIG010000565         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_2344953, whole genome shotgun sequence.
ACCESSION   DASRIG010000565 DASRIG010000000
VERSION     DASRIG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344953"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..864)
                     /gene="rph"
                     /locus_tag="VFB52_15030"
     CDS             complement(145..864)
                     /gene="rph"
                     /locus_tag="VFB52_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZO07701.1"
                     /translation="MTLSRSYARAPADIRPVTIDPGFVESADGSCLISVGKTRVICTA
                     SVSDQVPRWMIGRGKGWVTAEYAMLPASTGERKQRDVSKGKQDGRGVEIQRLIGRSLR
                     VVVDFKALGERSVYVDCDVLEADGGTRCASITGGFIALRQAFKKLLDEKAIETMPLTG
                     TIAAVSVGMVDGRALCDLDYSEDSTAEVDANVVMTGDGGLVEVQATAERTPLSRASLD
                     ELLALAEGGINRLREVQEQYA"
     gene            complement(938..2338)
                     /locus_tag="VFB52_15035"
     CDS             complement(938..2338)
                     /locus_tag="VFB52_15035"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HZO07702.1"
                     /translation="MSPQPLVAIGHGKNEPRGIKRVTRSSLHEGRFGRLFRGLDPAPE
                     LSNAELTALAKKMQEPKAGGAKLDNPKIPSAYTYFGQFVDHDITFDPVSSLQRRNDPD
                     ALTDFRTPRFDLDCVYGSGPADDPFLYVQPDRLRLLIDSSNKNGEPDLQRNAQGVALI
                     GDPRNDENIIVSQLQLAFLRFHNKVAERVAKDKSVPAARKFQETQKLVRWHYQWVILE
                     DFLPRIVGKGPLKDRVKRKGKGVEISLRWFKPKNNPFMPVEFSVAAYRYGHSQVRGRY
                     DLNQIVQNVPIFLPKEKVGELDDLRGFRPLPGAWTIDWSFFIGVPGQAKKPQLSRRID
                     AKLTKGLFDLPGFPQAESSLAFRNLKRGQALGLPSGQDVADAMELKPLGAGKLGAPVP
                     TPLWFYILKESGLKESGGGQHLGPVGGGIVAEVLLGLLKGDRHSFVNAQPGWRPTLGP
                     KAGKFTLADLIRFATN"
     gene            complement(2433..>2747)
                     /locus_tag="VFB52_15040"
     CDS             complement(2433..>2747)
                     /locus_tag="VFB52_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07703.1"
                     /translation="QRFLRRDSYNVHQPGSHYQVWEYMVELPGADGRPTRLTIKEKTF
                     KLKDPQIGDTVPVLVNRKRTKAAFDLKDPRIDAHTALKAKEKARMARDEVRFEDKRRG
                     LD"
BASE COUNT          376 a          883 c          961 g          527 t
ORIGIN      
        1 cgcgcgcctt gatcagcgcg ttggcctcga agctgtcgcc gtcctctggc ggcatctcga
       61 ccccctccgg cagcggccgc aggtcgaggt ccggcagcag ttcgagcatc tccgccagct
      121 tgtgccggtt gcccgtcgcc aggatcatgc gtactgctcc tgcacctccc gcaggcggtt
      181 gatgccgccc tcggccaggg cgagcagctc gtccagcgag gcgcgcgaga gcggcgtgcg
      241 ctcggcggtc gcctgcacct cgaccaggcc gccgtcgccg gtcatcacca cgttggcgtc
      301 gacctcggcg gtcgagtcct cgctgtagtc gaggtcgcag agcgcgcggc cgtcgaccat
      361 gccgacgctg accgcggcga tcgtcccggt cagcggcatc gtctcgatcg ctttttcgtc
      421 gagcagcttc ttgaacgcct ggcgcagggc gatgaagccg ccggtgatcg aggcgcagcg
      481 ggtgccgccg tcggcttcga gcacgtcgca gtcgacgtag acgctgcgtt cgccgagcgc
      541 cttgaagtcg acgacgaccc gcagcgagcg gccgatcagg cgctggatct ccaccccgcg
      601 cccgtcctgc ttgcccttcg agacgtcgcg ctgcttgcgc tcgccggtcg aggcgggcag
      661 catcgcgtac tcggcggtga cccagccttt gccgcggccg atcatccagc gcggcacctg
      721 gtcgctgacc gaggcggtgc agatcacccg cgttttgccg accgagatca ggcacgaccc
      781 gtcggcgctt tcgacgaagc cggggtcgat cgtgacggga cggatgtcgg cgggcgcgcg
      841 ggcgtagctg cgagagagcg tcatggggcg cgagtctttc acgcccggcg attggcgact
      901 tcctcaccca cgtgcgggag gaagtcgcca accccgctca gttggtcgcg aaccggatca
      961 ggtcggcgag ggtgaatttg ccggctttcg ggccgagggt cggccgccag cccggctggg
     1021 cgttgacgaa cgagtggcgg tcgcccttga gcagaccgag caggacctcg gcgacgatcc
     1081 cgccgccgac cgggccgagg tgctggccgc cgcccgactc tttgagcccc gactctttga
     1141 ggatgtagaa ccagagcggg gtcgggaccg gagcgccgag tttccccgcg cccagcggtt
     1201 tcagctccat cgcgtcggca acgtcctggc ccgagggcaa gccgagcgcc tggccgcgct
     1261 tgaggttgcg gaaggcgagc gaggactcgg cctgcgggaa gcccggcagg tcgaaaagcc
     1321 ctttcgtcag cttcgcgtcg attctcctgg aaagctgggg ttttttcgct tgcccgggga
     1381 cgccgatgaa gaacgaccag tcgatcgtcc aggcgcccgg cagcgggcgg aacccgcgca
     1441 ggtcgtcgag ttcgccgact ttttctttgg gcaggaagat cggcacgttc tggacgatct
     1501 ggttgaggtc gtagcggccg cggacctggc tgtggccgta gcggtaggcg gcaaccgaga
     1561 actcgaccgg catgaacgga ttgttttttg gtttgaacca gcgcagggag atctcgaccc
     1621 ctttgccttt gcgtttgacc cggtctttga gcgggccttt gccgacgatc cgcggcagga
     1681 agtcctcgag gatcacccac tggtagtgcc agcggaccag tttctgggtc tcctggaact
     1741 tgcgtgcggc cggcaccgat ttgtctttgg cgacgcgctc ggcgaccttg ttgtggaagc
     1801 gcaggaaagc gagctggagc tggctgacga tgatgttctc gtcgttgcgc ggatccccga
     1861 tcagggcgac gccctgggcg ttccgctgca ggtccggttc cccgtttttg ttgctgctgt
     1921 cgatcagcag ccgcagccgg tcgggctgca cgtagaggaa cgggtcgtcg gccggtccgg
     1981 agccgtagac gcagtcgagg tcgaagcgcg gcgtgcggaa gtcggtcagc gcatcggggt
     2041 cgttgcggcg ctgcaggctg gagaccgggt cgaaggtgat gtcgtggtcg acgaactggc
     2101 cgaagtaggt gtacgcggag gggattttcg ggttgtcgag tttggccccg ccggctttcg
     2161 gctcctgcat ttttttcgcc agcgccgtga gctcggcatt gctcagctcg ggcgccgggt
     2221 cgaggccgcg gaacagccgg ccgaagcgcc cctcgtggag gctcgagcgg gtgacccgtt
     2281 tgatccccct cggctcgttt ttcccgtgtc ctatggcgac cagcggctgc ggtgacatct
     2341 gcgcctccct gattggtttt gggttccctg cccaaaacca tcttgctccg cttctatgaa
     2401 cctcgctgtc gccctcggac cgctcgaccg aatcagtcga gtccccggcg cttgtcctcg
     2461 aagcgcacct cgtcccgggc catgcgggct ttttccttcg ccttgagcgc ggtgtgcgcg
     2521 tcgatcctcg gatccttcag gtcgaaggcg gccttggtgc gcttgcggtt gaccagcacc
     2581 gggacggtgt cgccgatctg gggatccttg agcttgaagg tcttctcctt gatcgtcagc
     2641 cgggtgggtc tgccgtcggc gccgggcagc tcgaccatgt actcccagac ttggtagtgg
     2701 gacccgggct gatggacgtt gtaggagtcc cggcgcagga accgctg
//
LOCUS       DASRIG010000566         7327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3378280, whole genome shotgun sequence.
ACCESSION   DASRIG010000566 DASRIG010000000
VERSION     DASRIG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378280"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /gene="lon"
                     /locus_tag="VFB52_15045"
     CDS             <1..1439
                     /gene="lon"
                     /locus_tag="VFB52_15045"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZO07704.1"
                     /translation="ILDEDHAGLEDVKRRITEFIAVRKLREERGVEDESRANGAILTL
                     VGPPGTGKTSIGESIANSLGREFVRISLGGLHDEAEIRGHRRTYIGALPGRIVRALRD
                     AGTMNPVILLDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHYLDVEVDLSEVVFIA
                     TANLIDTIPAPLLDRMELIAFDGYTTEEKLAIAKGYLLPRQLKRNGLQPEEVEVGDEI
                     LRKVISEYTREAGVRQLEREVGKLLRGAATRIASGNANGTVVLDEEALREALGRQRFF
                     QESVERTAIPGVSTGLAVTGTGGDVLFIEATAMEGDKGLVLTGQLGDVMKESARIALS
                     YARSHAEELGIDPSAFQREFHLHVPAGAIPKDGPSAGTAMTTALVSLLSGRPVKHTVG
                     MTGEVTLQGRVLPIGGLKQKVLAAHAAGLTEVILPERNRGDLDEVPEEVREQMTFHPV
                     MSVGEVLDLALEPAESQLTEQDHGVAAA"
     gene            1450..1758
                     /locus_tag="VFB52_15050"
     CDS             1450..1758
                     /locus_tag="VFB52_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07705.1"
                     /translation="MTPRCNNCQPVPPELRAAAGLLERRWTMATIYACSSGAARFNEF
                     KQSLPGVSPTTLSERLEQLEQAGIVKRHLVPGRPPHTEYELTERGQRIAAAVSGLLAH
                     "
     gene            1834..2631
                     /locus_tag="VFB52_15055"
     CDS             1834..2631
                     /locus_tag="VFB52_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZO07706.1"
                     /translation="MSDKPAIVLIHGFWVTPRSWENWIAHYEAKGYEVHAPAYPGLEV
                     EVEALNADPTPLKELTVDGIVAHLESVLAGIEGEPILIGHSAGGVFTQLLMDRGHGLA
                     GVAISSAPTEGVAVVPLSQIKSAFPVLKDPIHKDRAVPLDQKQWHYAFANTLSEEDSN
                     ALHERYAIPASGKIFWESALATLQPGHQGTYVDYKNDERAPLLFISGSEDHLMPPSVQ
                     ASNAKHYKSDTVTEVEVYEGRDHAIIAEAGWEEVADRALEWALAQSA"
     gene            2628..3416
                     /locus_tag="VFB52_15060"
     CDS             2628..3416
                     /locus_tag="VFB52_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZO07707.1"
                     /translation="MNLTITHIGGPTALIEFGGWRLLTDPTFDPAGGKYSFGLGTGSV
                     KTAAPALAAGEVGEIDAVLLSHDHHDDNLDAAGRTLLPGAGAVITTVPGAKRLGGNAR
                     GLAFWDKTTLEAPGKPTIEIVATPCRHGPPLSKPLVGDVIGFGLTWEGQQNGALWISG
                     DTVLYGGVREVAERLDVGTAIVHLGGVKFPISGPLHYTMTAAQAIELLGEIKPRTTIP
                     IHCEGWKHFREGRAEAERALAAAPQPLRESVRWLELGVATDVDV"
     gene            3448..4104
                     /locus_tag="VFB52_15065"
     CDS             3448..4104
                     /locus_tag="VFB52_15065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO07708.1"
                     /translation="MEGAPAENPAFALRRGEVVENPATGERVVILTDPFEHPDKVLVS
                     HLYVTPGGRVSVKHWHPTVTERFHVLSGQVGFQIGEEKHLLGPGQQAEVPPNTLHDWW
                     QVGEETAEVVVEVTPGDRFTELVGTFFGLARDGKVDKKGVPKPLQLAVSTTAYRDVIV
                     VASPPPWLQRIVFGVLAPIGRRRGLRPYYEEYLHTDVVVEPDPAALAMLTPDGRLEQA
                     "
     gene            complement(4123..6396)
                     /locus_tag="VFB52_15070"
     CDS             complement(4123..6396)
                     /locus_tag="VFB52_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZO07709.1"
                     /translation="MSDGEEKFSGIVYAAIAVSAVAMGFGIAFVAGFFLGHFTGDDEK
                     TVTVSAASPAEEEQAEAQIKAAPAFTSEELAAEPTEGWLTNGGSLSNARYSPLDEINT
                     DNVGDLKGVWHVNLDESGVATKYSAEATPIVYEGVMYVVSGADDVFAIDVKSGEQLWK
                     YEAKLDQKIDTVCCGWTSRGVAVGEGLVFVGQLDGSLVALDQQTGKVKWTTAVGKWQR
                     GETITSAPLYYDGRVYSGLSGGEFGIRGRLSAYDAQTGKLDWRFWTIPGPGEEGHETW
                     PDNNSWKHGGAPVWNTPAVDPELNTMYFSTGNASPDLNGSGRAGDNDFTTAIVAIDAT
                     TGKYKWDFQEVHHDIWDYDAPSPVVLWDGEVDGQPVKGLSQPGKTGWLYMLDRETGKP
                     IHGIVEKPVPQNKKEQQTAATQPIPQTPAFSRHDVTKAEYEEIAKLAKQAFKGKVKVR
                     HGQIFDPFGSEAAVVAPGPSGGTNWPPSSYNPDTENVYICGIDSYASYQTESLEKLKI
                     GETYLSSVLTLNGFGSYDGTLTAQNVSTGEIEWQKKFPKEACYSGTTTTAGNLVFSGR
                     NNGELVAYDAESGDELWSFQTGSGANTTPAIFEMDGTEYVAFYAGGNSLAASSHGDDL
                     WLFSLDGELGPVKAGGEAEQGEHAGEEAEGDVESDEVTPSEEEGVEEESAGGSATAGE
                     SVFADNCSICHGADGLGGNGGPDLTTQPLAQSLNGTIKQVTNGGGGMPAFKETLSTKE
                     IEDVAAYVVESIVGASK"
     gene            6544..7104
                     /locus_tag="VFB52_15075"
     CDS             6544..7104
                     /locus_tag="VFB52_15075"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZO07710.1"
                     /translation="MLRTQIAETAWSVFAERGFNDVTVNEVATATGISRATFFRYFSS
                     KEEAVFVALEALGEEVAAALGERPAGEDAWTALRRAFDAATPNYARNPARSLARLNLT
                     RETPSLRAHELERHQQWRRQIGAVLAGRLGCEQGGVRAEALVGAAISALDAATDRWAH
                     SGGELDLVALIDEAFAAIGDPHPTLG"
     gene            complement(7112..>7327)
                     /locus_tag="VFB52_15080"
     CDS             complement(7112..>7327)
                     /locus_tag="VFB52_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07711.1"
                     /translation="VAAEPDVGAALAAYERARVDRGALFVKESRRAAGIALPRSAFVR
                     RLRDTLLPLLPESARLRQFARFLDWTP"
BASE COUNT         1103 a         2545 c         2538 g         1141 t
ORIGIN      
        1 agatcctcga tgaggaccac gccggcctcg aggacgtgaa gaggcgcatc acggagttca
       61 tcgccgtgcg caagctgcgt gaggaacgcg gcgtcgagga cgagagccgc gccaacggcg
      121 cgatcctgac gctggtcggc cctcccggca ccggcaagac ctcgatcggc gagtcgatcg
      181 ccaacagcct cgggcgcgag ttcgtccgca tcagcttggg cgggctgcac gacgaggccg
      241 agatccgcgg tcaccgccgc acctacatcg gcgctctgcc gggacggatc gtgcgcgccc
      301 tgcgcgacgc cgggacgatg aacccggtga tcctgctcga cgaggtcgac aaggtcggcg
      361 ccgactggcg cggcgacccc agcgcggccc tgctcgaggt gctcgacccg gcccagaacc
      421 actcgttccg cgaccactac ctcgacgtcg aggtcgacct ctccgaggtc gtcttcatcg
      481 ccaccgcgaa cctgatcgac acgatcccgg ccccgttgct ggaccggatg gagctgatcg
      541 cgttcgacgg ctacacgacc gaggagaagc tggcgatcgc caagggctac ctgctgccgc
      601 ggcagttgaa gcgcaacggc ctgcagcccg aggaggtcga ggtcggcgac gagatcctgc
      661 gcaaggtgat ctccgagtac acgcgcgagg ccggcgtccg ccagctcgag cgtgaagtcg
      721 gcaagctgct gcgcggcgcc gcgacccgga tcgcttccgg caatgcgaac ggcaccgtgg
      781 tgctcgacga ggaggccctg cgcgaggcgc tcggccgcca gcgcttcttc caggagtcgg
      841 tcgagcggac cgcgatcccc ggtgtctcca ccgggctggc ggtgaccgga accggcggcg
      901 acgtcctctt catcgaggcg accgcgatgg agggcgacaa gggcctggtc ctgaccggcc
      961 agctcggcga cgtgatgaag gagtcggcgc ggatcgcgct ctcctacgca cgctcgcacg
     1021 ccgaggagct gggcatcgac ccaagcgcct tccagcgcga gttccacctg cacgtgcccg
     1081 ccggggcgat ccccaaggac ggtcccagcg ccggcacggc gatgaccacg gcgctggtct
     1141 cgctgctgag cgggcgaccg gtgaagcaca cggtcgggat gaccggcgag gtcaccctgc
     1201 agggccgggt gctgccgatc ggcgggttga agcagaaggt gctcgccgcc cacgcggccg
     1261 ggctgaccga ggtgatcctg cccgagcgca accgcggcga cctcgacgag gtccccgagg
     1321 aggtgcgcga gcagatgacc ttccacccgg tgatgagcgt cggcgaggtg ctcgacctgg
     1381 ccctggagcc ggcggaaagc cagctcaccg agcaggatca cggggtagcg gcagcgtagg
     1441 gtggcgaacg tgaccccgcg ctgcaacaac tgccagccgg tcccgcccga gctgcgggcg
     1501 gcggccggcc tgctcgagcg gcgctggacg atggcgacga tctacgcctg ttcgtccggc
     1561 gctgcccgtt tcaacgagtt caagcagtcg ctccccggcg tctcccccac caccctctcc
     1621 gagcgcctcg aacaactgga gcaggccggg atcgtcaagc gccacctcgt ccccggccgt
     1681 cccccccaca ccgagtacga gctgaccgag cgcggccagc gcatcgccgc cgccgtctcc
     1741 ggcctcctgg cgcactgact taacaagcgg gtctggacag gcggtttagg ctgagcgctc
     1801 ggccaaatcg ctcgctcaca ggaggctctt cccatgtcgg acaaacccgc aatcgtcctg
     1861 atccacggct tctgggtcac cccgcgcagc tgggagaact ggatcgcgca ctacgaggcg
     1921 aagggctacg aggtgcacgc gcccgcctac ccggggctgg aggtcgaggt cgaggcgctg
     1981 aacgccgacc cgacgccgct gaaggaactg accgtcgacg ggatcgtcgc ccacctcgag
     2041 agcgtgctcg ccgggatcga gggcgagccg atcctgatcg gccactcggc cggtggcgtc
     2101 ttcacccagc tcttgatgga ccgcggccac ggactcgccg gggtcgcgat cagctcggcg
     2161 ccgaccgagg gcgtcgccgt ggtgccgctc tcgcagatca aatccgcctt cccggtgctg
     2221 aaggacccga tccacaaaga ccgggccgtg ccgctcgacc agaagcagtg gcactacgcc
     2281 ttcgccaaca cgctcagcga ggaggactcg aacgcgctgc acgagcgcta tgcgatcccc
     2341 gcctccggga agatcttctg ggagagcgcc ctggcgaccc tgcagcccgg ccaccagggc
     2401 acctacgtcg actacaagaa cgacgagcgc gcgccgctgc tcttcatctc cggttccgag
     2461 gaccacctga tgccgcccag cgtccaggcc tccaacgcca agcactacaa gtccgacacg
     2521 gtcaccgagg tcgaggtcta cgagggccgc gaccacgcga tcatcgccga ggcgggctgg
     2581 gaggaagtcg ccgaccgggc gctcgagtgg gcgctggcgc agagcgcgtg aacctcacga
     2641 tcacccacat cggcggcccc accgcgctga tcgagttcgg cggctggcgg ctgctcaccg
     2701 acccgacctt cgacccggcc ggcggcaagt acagcttcgg cctcggcacc ggctcggtga
     2761 aaacggcggc gccggcactg gcggcgggcg aggtcggcga gatcgacgcg gtcctgctca
     2821 gccacgacca ccacgacgac aacctcgacg ccgccgggcg gacgctgctg ccgggcgcgg
     2881 gcgcggtgat caccaccgtg cccggggcga aacggctcgg cggcaacgcg cgcgggctcg
     2941 ccttctggga caagacgacg ctggaggcgc cgggcaagcc gacgatcgag atcgtcgcca
     3001 ccccctgccg ccacggaccg ccgctgagca agccgctggt gggcgacgtg atcgggttcg
     3061 ggctgacctg ggagggccag cagaacggcg ccctctggat ctccggcgac accgtgctct
     3121 acggcggcgt gcgcgaagtc gccgagcggc tcgacgtcgg caccgcgatc gtccacctcg
     3181 gcggggtcaa gttcccgatc tcgggaccgc tgcactacac gatgaccgcg gcgcaggcga
     3241 tcgagctgct gggcgagatc aagccgcgga cgacgatccc gatccactgc gagggctgga
     3301 agcacttccg cgaggggcgc gccgaagccg agcgcgcgct cgccgcggca ccgcagccgc
     3361 tgcgcgagag cgtgcgctgg ctggagctcg gcgtcgccac cgacgtcgac gtctgacgga
     3421 cccgccctct gagggtaggc tggcggcgtg gagggagccc cagcggagaa tcctgccttc
     3481 gccctgcgcc gcggcgaggt ggtcgagaac ccggcgaccg gggagcgcgt ggtcatcctc
     3541 accgacccgt tcgaacatcc cgacaaggtc ctcgtctccc acctctacgt cacccccggc
     3601 ggccgcgtct cggtcaagca ctggcacccg accgtgaccg agcgcttcca cgtgctctcc
     3661 ggccaggtcg gcttccagat cggcgaagag aagcacctgc tcggcccggg ccagcaggcc
     3721 gaggtcccgc cgaacaccct gcacgactgg tggcaggtcg gcgaggagac cgccgaggtc
     3781 gtcgtcgaag tgaccccggg cgaccgcttc accgagctgg tcggaacctt cttcggcctc
     3841 gcccgcgacg gcaaggtcga caagaaaggc gtgcccaagc cgctgcagct ggcggtcagc
     3901 acgaccgcct accgcgacgt gatcgtcgtc gcctcccccc cgccttggct gcagaggatc
     3961 gtcttcggcg tgctcgcccc gatcggccgc cgccgcgggc tcaggccgta ctacgaggag
     4021 tacctgcaca ccgacgtggt cgtcgagccc gaccccgccg cgctggcgat gctgaccccc
     4081 gacgggcggc tcgagcaggc ctagccgccc ggcggcgagg cgctatttgc ttgcgccgac
     4141 gatcgattcg accacgtagg cggcgacgtc ttcgatttcc ttggtcgaga gcgtttcttt
     4201 gaacgccggc atcccgccgc cgccgttggt cacctgtttg atcgtcccgt tcagcgactg
     4261 cgccagcggc tgcgtggtca ggtcggggcc gccgttgccg ccgagcccgt cggcgccgtg
     4321 gcagatcgag cagttgtcgg cgaacaccga ttcgccggcc gtcgcgctgc cgcccgccga
     4381 ttcctcttcg accccttctt cttcggatgg agtcacttcg tcggactcga cgtcgccttc
     4441 ggcttcctcg ccagcatgct cgccctgttc ggcttcgccg cccgctttca ccggcccgag
     4501 ctcaccgtcg agcgagaaga gccaaaggtc gtcgccgtgc gagcttgccg ccagcgagtt
     4561 gccgccggcg tagaaggcga cgtactcggt gccgtccatt tcgaagatcg ccggggtcgt
     4621 gttggcgccg gagccggtct ggaaggacca gagctcgtca ccgctttcgg cgtcgtaggc
     4681 aaccagctca ccgttgttgc ggccgctgaa gacgaggttg ccggcggtcg tcgtggtccc
     4741 gctgtaacag gcttctttgg ggaatttctt ctgccactcg atctcgccgg tgctgacgtt
     4801 ctgcgcggtc agcgtgccgt cgtaggagcc gaacccgttc agggtgagca cgctcgagag
     4861 gtaggtctcg ccgattttca gtttttcgag gctctcggtc tggtagctgg cgtaggagtc
     4921 gatcccgcag atgtagacgt tttcggtgtc cgggttgtag ctcgacggcg gccagttggt
     4981 accgccggac gggcccggcg cgaccaccgc cgcttcgctg ccgaacgggt cgaagatctg
     5041 cccgtggcgg actttgactt tgcctttgaa cgcctgtttg gcgagtttcg cgatttcttc
     5101 gtactcggct ttggtcacgt cgtgccggga gaaggcgggc gtctgcggga tcggctgggt
     5161 cgccgccgtc tgctgctctt ttttgttctg cgggaccggt ttctcgacga tcccgtggat
     5221 cggtttgccg gtttcccggt cgagcatgta gagccagccg gtcttgcccg gctgcgagag
     5281 ccctttgacc ggctgcccgt cgacttcgcc gtcccagagc acgaccgggc tgggtgcgtc
     5341 gtagtcccag atgtcgtggt ggacctcctg gaagtcccac ttgtatttgc cggtggtggc
     5401 gtcgatcgcg acgatcgcgg tggtgaagtc gttgtcgccg gcgcggccgg agccgttcag
     5461 gtcaggtgag gcgttgccgg tcgagaagta catcgtgttc agctcggggt cgaccgcggg
     5521 cgtgttccag accggggcgc cgccgtgttt ccaggagttg ttgtcgggcc aggtctcgtg
     5581 gccctcttca ccggggccgg ggatcgtcca gaagcgccag tcgagtttgc cggtctgggc
     5641 gtcgtaggcg ctgagccggc cgcggatccc gaactcgccg ccggagaggc cggagtagac
     5701 gcggccgtcg tagtagagcg gcgccgaggt gatcgtctcg ccgcgctgcc atttgccgac
     5761 cgcggtcgtc catttgactt tgccggtctg ctggtcgagc gcgaccagcg agccgtcgag
     5821 ctggccgacg aagaccaggc cctcgccgac cgcgaccccg cggctggtcc agccgcagca
     5881 gaccgtgtcg attttctggt ccagtttcgc ttcgtacttc cacagctgtt cgccgctctt
     5941 cacgtcgatc gcgaagacgt cgtcggcgcc gctgaccacg tacatcacgc cttcgtagac
     6001 gatcggcgtc gcctcggccg agtacttggt ggcgacgccg gattcgtcga ggttgacgtg
     6061 ccagacgcct ttcaggtcgc cgacgttgtc ggtgttgatt tcgtcgagcg gcgagtagcg
     6121 ggcgttggag agggagccgc cgttggtcag ccagccctcg gtcggttcgg cggcgagttc
     6181 ctcggaggtg aatgcgggtg ccgccttgat ctgcgcttcc gcctgctctt cttcggcggg
     6241 cgaggcggcg gagacggtga cggtcttctc gtcgtcgccg gtgaagtggc cgaggaagaa
     6301 accggcgacg aaggcgatcc cgaaacccat ggcgaccgcg ctcaccgcga tcgcggcgta
     6361 aacgatgccg gagaactttt cctcgccgtc cgacatgtct ctcctaactg ttgagcctca
     6421 gtctcacttt agatacgcag tgtaaccaac tagactcccc gagtggccac gggggacgcc
     6481 aacacgcctg agcacccttc tgcgaactca ccggtacccg atttgcgggc gcgtactcgg
     6541 gacgtgctgc gcacgcagat cgccgagacc gcctggagcg tgttcgccga acgcggcttc
     6601 aacgacgtca ccgtcaacga ggtcgcgacc gcgaccggga tctcgcgcgc gaccttcttc
     6661 cgttacttct cctccaagga ggaggcggtg ttcgttgcgc tcgaggcgct gggcgaggaa
     6721 gtcgccgcgg cgctgggcga gcggccggcc ggcgaggacg cctggacggc gctgcggcgc
     6781 gccttcgacg cggcgacccc gaactacgcg cgcaacccgg cccgctcgct ggcgcggctc
     6841 aacctgaccc gggaaacgcc ctcgctgcgc gcccacgaac tcgagcgcca ccagcagtgg
     6901 cggcggcaga tcggggcggt gctggccggc aggctcggct gcgagcaggg cggcgtgcgg
     6961 gcggaagctc tggtcggcgc cgcgatcagc gcgctcgacg ccgccaccga ccgctgggcc
     7021 cactccggcg gcgagctcga cctggtcgcg ctgatcgacg aagcgttcgc cgcgatcggc
     7081 gacccccacc ccaccctcgg ctgagcccga actacggggt ccagtcgagg aagcgggcga
     7141 actggcgcag gcgcgccgat tcgggcaaga gcggcagcag ggtgtcgcgc aggcggcgga
     7201 cgaaggccga gcgcggcagg gcgatgccgg cggcgcggcg cgactccttg acgaagagcg
     7261 ccccacggtc gacgcgggcg cgctcgtagg cggcgagcgc ggcaccgacg tcgggctcgg
     7321 cggcgac
//
LOCUS       DASRIG010000567         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_507568, whole genome shotgun sequence.
ACCESSION   DASRIG010000567 DASRIG010000000
VERSION     DASRIG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507568"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFB52_15085"
     CDS             <1..237
                     /locus_tag="VFB52_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZO07712.1"
                     /translation="VATEPISSGEKVVGVYLDDGSTVEVEAKGDVPLGHKIAVRPTEG
                     DNSVIKYGVKIGDAPEGLEVGDYVHTHNLKSARW"
     gene            240..1391
                     /locus_tag="VFB52_15090"
     CDS             240..1391
                     /locus_tag="VFB52_15090"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HZO07713.1"
                     /translation="MDLLGYRRPNGRVGIRNYVAIIPVDDISNAACVAVAAHVRGTLA
                     LPHSGGRLQYGEDLEVHFRTIIGTGRNPNVAAAVVIGIEEKWSRRVAEGIAATGKPVE
                     WFSIEGNGDFETVRRASWAAKQMVHEASELRREPFDLSELWVSLKCGESDTTTGLGSC
                     PTVAEAVDPLIDAGATVFFGETSELTGGEHLIADRMATPELREKFMAMYDDYVAEIES
                     SGSNLLGSQPTEGNIAGGLSTIEEKALGNIEKTGTKEVIGVLGPAEEPENGPGLYFMD
                     SSSAAAECVTLMAAGGAVLHLFPTGQGNVIGNPIEPVVKISANPSTVTTMSEHIDLDV
                     SGLLRRELTLDQAGEEMLSMIARTANGRVTAAEALGHEEFSMTKLYRSA"
     gene            complement(1392..1772)
                     /locus_tag="VFB52_15095"
     CDS             complement(1392..1772)
                     /locus_tag="VFB52_15095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZO07714.1"
                     /translation="MIKGSLEGLELAALPGRLAADPMPASAAEGVATRLVEVREQRRN
                     AHRHPDSPELIQVLSGRGEHWQDGEREPLRPGDLVLVPTGRPHVSLAAPGEVLRLLCF
                     FPVADVAAATEELDAEVAIATSAP"
     gene            complement(1769..>2724)
                     /locus_tag="VFB52_15100"
     CDS             complement(1769..>2724)
                     /locus_tag="VFB52_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZO07715.1"
                     /translation="DAAKRASFQEEFGVPRGAATFEELLADEEVEGLIVTTPNDTHRP
                     LIVQALEAGKPVYVDKPIAHTLADAVAVADAAATTGLPFAVGHSARRLSGSRQMKGWI
                     EDGRLGDLSLVEANFSNERGLELTTDTWRYFADKSPGGTMIQLGVHHADNLQYLVGPP
                     VAVSAHARRLYTDAEVPDAVMAIVEFEGGVLGYIGAGWASPGIYTINLQGTKANLRYD
                     LDFTHWDESQQADQWSSLVSQGYGESVRSPVELGPTDMFREQLEEFALAIRGEAEIEV
                     GAVEGLRALAVVHASIASSERGGAAVEIAELLDQAGAGALA"
BASE COUNT          416 a          984 c          962 g          362 t
ORIGIN      
        1 gtcgccacgg agccgatcag ctccggcgag aaggtggtcg gcgtctacct cgacgacggc
       61 tccacggtcg aggtcgaggc caagggcgac gtcccgctcg gccacaagat cgccgtccgc
      121 cccaccgagg gcgacaactc cgtgatcaag tacggggtca agatcggcga cgcccccgag
      181 ggcctcgagg tcggcgacta cgtccacacc cacaacctca agagcgcgag gtggtagccg
      241 tggacctgct cggttacaga cgccccaacg gccgcgtcgg gatccgcaac tacgtcgcga
      301 tcatccccgt cgacgacatc tccaacgccg cctgcgtcgc ggttgccgcc cacgtgcgcg
      361 gcacgctcgc gctcccccac tccggcggcc gcctgcagta cggcgaggac ctcgaggtcc
      421 acttccgcac gatcatcggc accggccgca accccaacgt cgccgccgcc gtggtgatcg
      481 ggatcgagga gaagtggtcg cgccgggtcg ccgagggcat cgccgccacc ggcaagccgg
      541 tcgagtggtt ctcgatcgag ggcaacggcg acttcgagac cgtgcgccgt gcctcctggg
      601 cggcgaagca gatggtccac gaggcgagcg agctgcgccg cgagcccttc gatctctccg
      661 agctctgggt cagcctcaag tgcggcgagt ccgacacgac caccgggctc ggctcctgcc
      721 cgaccgtcgc cgaggcggtc gacccgctga tcgatgccgg cgccacggtc ttcttcggcg
      781 agacctcgga gctgaccggc ggcgagcatc tgatcgccga ccggatggcg acgccggagc
      841 tgcgcgagaa gttcatggcg atgtacgacg actacgtcgc cgagatcgaa agcagcggct
      901 ccaacctgct cggctcccag ccgaccgagg gcaacatcgc cggcggcctc tcgacgatcg
      961 aggagaaggc gctcggcaac atcgagaaga ccggcaccaa agaggtgatc ggggtgctcg
     1021 gcccggccga ggaacccgag aacggccccg ggctgtactt catggacagc tcctcggcgg
     1081 cggccgagtg cgtgaccctg atggccgccg gcggcgccgt cctgcacctc ttcccgaccg
     1141 gccagggcaa cgtgatcggc aacccgatcg agccggtcgt gaagatcagc gccaacccga
     1201 gcaccgtgac gacgatgagc gagcacatcg acctcgacgt cagcggcctg ctgcggcggg
     1261 aactgacgct cgaccaggcg ggcgaggaga tgctctcgat gatcgcccgc accgccaacg
     1321 ggcgggtgac cgcggccgag gcactcggcc acgaagagtt ctcgatgacg aagctctacc
     1381 gcagcgctta gctagggggc cgaggtggcg atcgccacct cggcatccag ctcctcggtc
     1441 gcggcggcga cgtcggcaac cgggaagaag cagagcaggc gcaggacctc gccgggggcg
     1501 gcgaggctga cgtgcggccg cccggtcggg accagcacca ggtcgccggg gcgcagcggc
     1561 tcgcgctcgc cgtcctgcca gtgctcgccg cggccggaga gcacctggat cagctccggg
     1621 gagtcggggt ggcggtgggc gttgcgacgc tgctcgcgga cctcgaccag ccgcgtcgcg
     1681 acgccctccg ccgcgctcgc cggcatgggg tcggcggcca gccgcccggg cagggcggcc
     1741 agctccaatc cctccagcga ccccttgatc atgcgagcgc gcccgcgccc gcctggtcga
     1801 gcagctcggc gatctcgacc gcggcgccac cgcgctcgga ggaggcgatg ctggcgtgca
     1861 cgaccgccag cgcccgcagc ccctcgaccg cgccgacctc gatctcggcc tcgccgcgga
     1921 tcgccagcgc gaactcctcg agctgctcgc ggaacatgtc ggtcggcccg agctcgaccg
     1981 gcgaacgcac cgactcgccg tagccctgcg agaccagcga gctccactgg tcggcctgct
     2041 gggattcgtc ccagtgggtg aagtcgaggt cgtagcgcag gttcgccttc gtgccctgca
     2101 ggttgatcgt gtagatgccc ggcgaggccc agccggcgcc gatgtagccg agcacgccgc
     2161 cctcgaactc gacgatcgcc atcaccgcgt cgggcacctc ggcgtcggtg tagagccggc
     2221 gcgcgtgggc gctgaccgcc accggcggcc cgaccaggta ctggaggttg tcggcgtggt
     2281 ggacgccgag ctggatcatc gtcccgcccg ggctcttgtc ggcgaagtag cgccaggtat
     2341 cggtggtcag ctcgagcccg cgctcgttgg agaagttggc ctcgaccagc gagaggtcgc
     2401 cgagccggcc gtcctcgatc caccccttca tctgccgcga gccggagagc cggcgggcgc
     2461 tgtggccgac cgcgaacggc agcccggtcg tggcagccgc gtcggcgacc gcgaccgcgt
     2521 cggccagggt gtgggcgatc ggcttgtcga cgtagaccgg cttgccggcc tccagcgcct
     2581 gcacgatcag cggccggtgg gtgtcgttcg gggtggtgac gatcagcccc tcgacctcct
     2641 cgtcggcgag cagctcctcg aaggtcgcgg cgccgcgggg cacgccgaac tcctcctgga
     2701 aggaggcgcg cttggcggcg tcgc
//
LOCUS       DASRIG010000568         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_508335, whole genome shotgun sequence.
ACCESSION   DASRIG010000568 DASRIG010000000
VERSION     DASRIG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508335"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFB52_15105"
     CDS             complement(<1..402)
                     /locus_tag="VFB52_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07716.1"
                     /translation="MLDLGCYLLGVLKLGIVAGALGFAAYRLRQRFLAGWGGSPARLV
                     EVVLGVALLTWLSELLGAVDLFYASTLVAGSVLIGLAGWFVPGGDPGGDAEDGAAHRP
                     QASPPGAGPAGPHPLALLITLGVIAVVVFHWA"
     gene            complement(424..786)
                     /locus_tag="VFB52_15110"
     CDS             complement(424..786)
                     /locus_tag="VFB52_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZO07717.1"
                     /translation="MAARAQARRKPAPARRRPARRPAPRSGPSRIKWDRVGRVALTLV
                     LAAVLYSYLNPALDFVKTYTGTTEAKAHLHELQAESRRLHDRVQSSEDPIVIDQRARS
                     LGMVEEGERPVVIRGLNR"
     gene            complement(827..2119)
                     /gene="eno"
                     /locus_tag="VFB52_15115"
     CDS             complement(827..2119)
                     /gene="eno"
                     /locus_tag="VFB52_15115"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZO07718.1"
                     /translation="MSAIASIHARQILDSRGNPTVEVEVELESGASGLAAVPSGASTG
                     EFEAVELRDGGDAWAGKGVTTAVANVNGEIAAALAGFEATDQAGLDAKMIELDGTPNK
                     GRLGANALLGVSLAVAKAAAADAGQPLYAYLAELYGGGEPQVLPVPMMNVLNGGAHAD
                     NSVDFQEFMIVPAGAASFSECLRVGAEVFHALKKTLSAQGHSTAGGDEGGFAPNLESN
                     EAALQAVLAGIEAAGYTPGTDVFIALDPATSEVYEGGAYVLEHEGRSLSSEEMAAYWE
                     DACSRYPIVSIEDGMDEEDWDGWKLLTEKLGERVQLVGDDLFVTNPERLRRGIELGVG
                     NSILVKVNQIGTLSETLEAIRTAIDAGYTAVISHRSGETEDTTIADLAVATGAGQIKT
                     GAPSRSDRVAKYNRLLRIEEELGSRASFPGLQAFVQRR"
     gene            complement(2182..3486)
                     /locus_tag="VFB52_15120"
     CDS             complement(2182..3486)
                     /locus_tag="VFB52_15120"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZO07719.1"
                     /translation="MGAKTGQMGKTAGRKRLALILFGVLFVVLFLGFAISQGLGRDSV
                     PEGDVALVEDGPEGENTVSEADFKRAILQQALQGGLKKVPKEGSTRFEELKGAALGEL
                     LDFIWIRGEAEELDLTATKKQMDEELEKIKEQNFPTPAAYKEFLETSKLTQADVDKRV
                     ELQVLSGQITEAVQGNAGVPTNSEIEDYYNAAKATQFTTKPSRDIRIITNKDKAEVEA
                     AVAELEKDNSAASWKKVAQKYSSDPTTKSKGGLQEGLTEELLATAGPLKKEIFEGATN
                     ELRGPIQFQGNWTVIEVVKLNPEKVQTLGEARQQISSTLQTQNQEAFFERFVANYQSK
                     WESRTICASGFEIERCSNFKGDGHPSTANPACYEADPKTPATECPSVVTPISPTLPGK
                     VSLLTPKGEPFPQRPVPVPNGEGAAEGVNEVLPEGVEAAPGE"
     gene            complement(3548..>4459)
                     /locus_tag="VFB52_15125"
     CDS             complement(3548..>4459)
                     /locus_tag="VFB52_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HZO07720.1"
                     /translation="ALEETMLGFLRGEADCLVTTTIIESGLDIPTANTLIVERSDQLG
                     LAQAYQIRGRVGRSRERAFAYLLYPSAEALSEEAAARLSTLADHTELGSGFRIAMRDL
                     DIRGAGNLLGDEQSGHVAAVGFELYCQMIDEAVAEAAAAQGEGAEREEAPEPVRLDVP
                     VDAYLPASFVPFEAAKIDVHRRIVAAREPGQLRAIRDELADRFGPLPPQAENLLKLQQ
                     ARIELGLAGARSVEFRGGRLSVTGVELDSEGAGVLAEQVEGALYEWREQTAALRVAGD
                     PDERLEAVLALAKGLREAQRAAEATAA"
BASE COUNT          609 a         1506 c         1566 g          778 t
ORIGIN      
        1 cgcccagtgg aacaccacga ccgcgatgac gccgagcgtg atcagcaagg cgagggggtg
       61 tgggccggcg ggtcctgccc cgggtgggga tgcctgcggg cggtgcgcgg ctccgtcctc
      121 ggcatcccca cccgggtcac cccccggcac aaaccacccc gccagaccga tcaggaccga
      181 gccggcaacc agggtgctgg cgtagaacag gtccacagca ccaagaagct cgctcagcca
      241 cgtaagcagc gcgacgccca gcactacctc gaccaagcga gcggggctcc ctccccaccc
      301 agccaaaaag cgttggcgca gccgatacgc agcgaagccc agggctccgg cgacgatccc
      361 gagtttgagg acccccagca ggtagcagcc gaggtcaagc atgggggtgc ctcgcggcgg
      421 gggtcagcgg ttgaggccgc ggatcacgac cggccgctcg ccttcctcga ccatcccgag
      481 cgaacgggcg cgctggtcga tcacgatcgg gtcctcggag gactggacgc ggtcgtggag
      541 gcgccggctt tccgcctgca gctcgtgcag atgcgctttc gcctcggtcg tgccggtgta
      601 ggtcttgacg aagtcgagcg ccgggttcag atacgagtag agcacggcgg cgaggaccag
      661 ggtcagggcg acgcggccga cccggtccca cttgatccgg ctcggcccgc tgcgcggcgc
      721 gggccggcgg gccgggcgcc gacgggctgg agccggcttg cggcgggcct gggcgcgtgc
      781 tgccatgccc ggtcctttgc ggccgagctg cgcattcctt catttgttag cgacgctgaa
      841 caaacgcctg caaaccggga aagctggccc gtgagccgag ctcctcctcg atccgcagca
      901 ggcggttgta cttggcgacg cgatcggagc gcgagggcgc cccggtcttg atctggccgg
      961 cgccggtggc gacggcgagg tcggcgatcg tcgtgtcctc ggtctcgccg gagcggtgcg
     1021 agatcaccgc cgtgtagcca gcgtcgatgg cggtgcggat cgcctccagg gtctcgctca
     1081 gggtgccgat ctggttgacc ttgaccagga tcgagttgcc gaccccaagc tcgatcccgc
     1141 ggcgcaggcg ctcgggattg gtgacgaaga ggtcgtcgcc gaccagctgc acacgctcgc
     1201 cgagcttctc ggtcagcagc ttccagccgt cccagtcctc ctcgtccatg ccgtcctcga
     1261 tcgagacgat cgggtagcgc gagcaggcgt cctcccagta ggcggccatc tcctcggacg
     1321 agagactgcg gccctcgtgc tcgagcacgt aggcgccacc ctcgtagacc tcgctggtcg
     1381 ccggatcgag ggcgatgaag acgtcggtgc cgggggtgta gccggcggcc tcgatcccgg
     1441 cgaggacggc ctgcagggca gcctcgttgg actcgaggtt gggagcgaag ccgccctcgt
     1501 cgccgccggc ggtcgagtgg ccctgggcgg agagcgtctt cttgagcgcg tggaagacct
     1561 cggcgccgac ccgcaggcac tcggagaagc tggcggcccc cgccggaacg atcatgaact
     1621 cctggaagtc gaccgagttg tcggcatggg cgccaccgtt cagcacgttc atcatcggca
     1681 ccggcagcac ctgcggctca ccgccgccgt agagctcggc aaggtaggcg tagagcggct
     1741 ggccggcgtc ggccgcggcg gccttggcga ccgccagcga gacgccgagc agggcattgg
     1801 cgccgagccg gcccttgttc ggggtgccgt cgagctcgat catcttcgcg tcgaggccgg
     1861 cctggtcggt ggcctcgaag ccggccagcg cggcggcgat ctcgccgttc acgttggcga
     1921 ccgcggtggt cacgcccttg ccggcccagg cgtcgccgcc gtcgcgcagc tcgaccgcct
     1981 cgaactcgcc ggtcgaggcc cccgagggaa ccgcggcgag gccgctggcg cccgactcga
     2041 gctcgacctc gacctcgacg gtcgggttgc cgcgcgagtc gaggatctgg cgcgcgtgaa
     2101 tggaggcgat agcgctcacg gaggcggcag tctattcaca ggatgggcgc ccgcgacggc
     2161 tgcccgggtg acccggcggc gctattcgcc cggagcggct tcgacgcctt cgggcaggac
     2221 ttcgttgacg ccttcggcgg cgccttcccc gttgggcacc ggcaccgggc gctgcgggaa
     2281 gggttcgcct ttcggcgtca gcagcgagac tttgccgggc agggtcggcg agatcggcgt
     2341 gaccaccgac gggcattcgg tcgccggagt tttcgggtcg gcttcgtagc aggccgggtt
     2401 ggcggtgctc ggatgcccgt cgcccttgaa gttcgagcag cgttcgatct cgaagccgga
     2461 ggcgcagatc gtgcgcgatt cccacttgct ctggtagttg gcgacgaacc gttcgaagaa
     2521 ggcttcctgg ttctgcgtct gcagcgtgct gctgatctgc tggcgcgctt cgccgagcgt
     2581 ctgcactttc tccgggttca gtttcaccac ttcgatcacc gtccagttgc cctggaactg
     2641 gatcggcccg cgcagttcgt tggtggcgcc ctcgaagatc tcttttttca gcggcccggc
     2701 ggtggcgaga agttcttcgg tcaggccttc ctggaggcca cctttcgatt tcgtcgtcgg
     2761 gtccgaggag tatttctgcg cgactttttt ccagctcgcg gccgagttgt ctttttccag
     2821 ctcggccact gcggcttcga cttccgcttt gtctttgttg gtgatgatgc ggatgtcacg
     2881 actcggtttg gtcgtgaact gagtcgcctt cgccgcgttg tagtaatcct cgatttccga
     2941 gttggtcgga accccggcgt tgccctggac cgcttcggtg atctggccgc tcagcacctg
     3001 cagctcgacc cgcttgtcga cgtcggcctg ggtcagtttt gaggtttcga ggaattcttt
     3061 gtaggcggcc ggcgtgggga agttctgttc tttgattttt tccagttctt cgtccatctg
     3121 ttttttcgtc gccgtgaggt cgagttcttc ggcctcgccg cggatccaga tgaagtcgag
     3181 gagttcgccc agcgccgcgc ccttcagttc ttcgaagcgg gtgctgccct ctttcgggac
     3241 ttttttcagc ccgccctgga gcgcctgctg gaggatcgcc cgcttgaagt cggcttcgct
     3301 gaccgtgttt tcgccctcgg gaccgtcttc gaccagcgcg acgtcgccct cggggacgct
     3361 gtcgcggccg agtccctggg agatcgcgaa gccgaggaag agcacgacaa aaaggacgcc
     3421 gaagagaatc agcgccagcc gcttcctacc ggccgtcttg cccatctgcc ccgtcttggc
     3481 tcccacgcag cgggcagcat agtcaaattt ggttaggccc agctaaatgg cgccgcgcct
     3541 ggtcgaatca ggccgccgtc gcctcggccg cgcgctgcgc ctcgcgcagc cctttggcca
     3601 gggccaggac cgcttccaag cgctcgtcgg ggtcgccggc aacgcgtaga gccgcggtct
     3661 gctcgcgcca ttcgtagagc gcgccctcga cctgctcggc cagtaccccg gccccctcgg
     3721 agtccagctc gacgccggtg acgctgagcc ggccgccgcg gaactcgacg ctgcgcgcgc
     3781 ccgcgaggcc gagttcgatc cgtgcctgct gcagcttcag caggttctcg gcctgcggcg
     3841 gcagcggtcc gaagcggtcg gcgagctcgt cgcggatcgc ccgcagctgg cccggctcgc
     3901 gggcggcgac gatgcgacgg tggacgtcga tcttggcggc ctcgaagggg acgaagctgg
     3961 ccggcaggta ggcgtcgacg gggacgtcga ggcggaccgg ctccggcgcc tcctcgcgtt
     4021 cggccccctc cccctgcgcg gccgcggcct cggcgaccgc ctcgtcgatc atctggcagt
     4081 agagctcgaa gccgaccgcg gcgacgtgcc ccgactgctc gtcgccgagc aggttgccgg
     4141 cgccgcggat gtcgaggtcg cgcatcgcga tccggaagcc ggagccgagc tcggtgtggt
     4201 cggcgagggt cgagagccgc gccgcggcct cctcgctcag cgcctcggcc gacgggtaca
     4261 gcaggtaggc gaaggcgcgc tcgcgactgc gaccgacgcg gccgcggatc tggtaggcct
     4321 gcgcgagccc gagctggtcg gagcgctcga cgatcagcgt gttcgcggtc gggatgtcga
     4381 ggccggactc gatgatcgtc gtcgtcacca ggcagtcggc ctcgccgcgc aggaagccga
     4441 gcatcgtttc ctccagcgc
//
LOCUS       DASRIG010000569         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate SMAG_U10104
            k141_3381645, whole genome shotgun sequence.
ACCESSION   DASRIG010000569 DASRIG010000000
VERSION     DASRIG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963632
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (soil metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Solirubrobacterales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:35:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381645"
                     /isolate="SMAG_U10104"
                     /isolation_source="soil"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFB52_15130"
     CDS             <1..530
                     /locus_tag="VFB52_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZO07721.1"
                     /translation="RDIPGVHGDEIAAILAREHPAVRILMLTAAARLADKVDGFELGA
                     DDYLTKPFELQELILRLRSLARRPPAVAPPVMEFADLRLDPFRREVYRAGRYLKLTRK
                     QFAVLELLMRADGGVVSAETMLEKVWDENADPFSSAPRVTMSTLRKALGPPDLIVTVT
                     GAGYRLGPPEDADAD"
     gene            562..1635
                     /locus_tag="VFB52_15135"
     CDS             562..1635
                     /locus_tag="VFB52_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZO07722.1"
                     /translation="MSARLRLTLSYAVFLVVAGSVALFGFYMALHLGLGFKLQSADIT
                     SRGELRDAMLVAAAAALLGLAAVGLGGGWLLAGWILRPLDDINQAARIAATGRLDHRI
                     RLTGRSDEFGQLADNFDHMLERLHDAFLNQERFAANASHELRTPLAVTATMLDVARTD
                     PEGRYDPALLERLQLTNDRAIELTEALLRLSDANAVTAVSEPVDLAALAGGAVEELEA
                     EAERRHVQLHAKLEAAPSVGDPALLRQLVGNLAENAVRHNRPEGEAWLATHIDAVSSR
                     AVVQVENTGPSYTEETAAQLVQPFLRGAGRTAAQNGDRGYGLGLALVARIVNVHDGEL
                     TISPRPGGGLIVTVALPPPAYRR"
     gene            complement(1623..2213)
                     /locus_tag="VFB52_15140"
     CDS             complement(1623..2213)
                     /locus_tag="VFB52_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZO07723.1"
                     /translation="MPDVTADISITLDGYASGRGQSREHPFGDLDADRLHSWQFDHIE
                     ENEEERAVVTVAGAYIMGRKMFSSEEGEWDLDWRGYWGPNPPYHAPTFVLCHRPRPSI
                     EMEGGTVFHFVTDGIEAAMERAREAAGEKTVSIAGGPGTLNQYLAAGLVDELRLHVAP
                     FVAGGGERLFDGVGDLQLKPASSRTTPYVTHLVYRR"
     gene            2313..2810
                     /locus_tag="VFB52_15145"
     CDS             2313..2810
                     /locus_tag="VFB52_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZO07724.1"
                     /translation="MSDGRSGCPINLSIEVIGDRWSLLVIRDMMFGNRHHFGELLAGS
                     EEGIASNILADRLRRLTEQGLVSRAPDPSHKQKVIYNLTEPAIELVPVIAQLGAWGRR
                     HRPVSRELSIRAELLEQGGPELWEEFMAELREQHLGIPRPAGAESVFDRLQAAYEAEL
                     AKAGQ"
     gene            2892..3467
                     /locus_tag="VFB52_15150"
     CDS             2892..3467
                     /locus_tag="VFB52_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZO07725.1"
                     /translation="MSATYTFDVFSSLDGFGSASGDWTGYWGKQGPELLDHRLGLYDE
                     EQRMVFGATTYRAFARMLAESTEDSEVRDPWVTRMRNLPATVVSTTLDGPLDWPDASL
                     VSGDAVDVVARLKEESAVPLRSHGSLSMNRALMAAGLVDRVQVTLFPVITGQTGEEPI
                     FGGAADFDLELIETRTLDGDIQELVYRPTLH"
     gene            complement(3476..>4659)
                     /locus_tag="VFB52_15155"
     CDS             complement(3476..>4659)
                     /locus_tag="VFB52_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZO07726.1"
                     /translation="ILNVTSGTNPNDPETAESDARKAADYRSFLSKDALQGVRLGYDP
                     AAFTNLDGETSSLALAEEQFDDLEAAGAEIIEMPTPLPASDWTGGVDVIAWVVEAYGR
                     WLEEHPGTGFANGKEMFTSPLHSPINAWKTEWDGPILTTADEEKLVKGRWALGQEIMD
                     WMDASDVDATISPGRTAPLDDAGGISGTSLNGYGGWNSNVSSVSNLPSVNVPTGLDAS
                     GSPNGIQFSGRAWDDGKLLGYAYALEQQIGDGQVLSKAAPKLAYDPTATPRPIEIKVP
                     DKPITEGPSTPPPAVLGFGKPALFSKQLRLSGTGRIRVAVGCTGSVPCGMQVTLRIGK
                     KALGSKTLTIEGGERKPIVFKLSRKRYLTALRAEKLRVIVAPVGPSASGKVTKVLALL
                     H"
BASE COUNT          676 a         1667 c         1584 g          732 t
ORIGIN      
        1 atcgcgacat ccccggggtc cacggcgacg agatcgccgc gatcctggcc cgcgagcacc
       61 cggcggtgcg gatcctgatg ctgaccgccg cggcgcggct ggcggacaag gtcgacgggt
      121 tcgagctcgg cgccgacgac tacctgacca agccgttcga gctgcaggag ctgatcctgc
      181 ggctgcgctc gctggcccgc cgcccgcccg cggtcgcgcc gccggtgatg gagttcgccg
      241 acctgcgcct cgacccgttc cggcgcgagg tctaccgcgc cggtcgctac ctgaagctga
      301 cccgcaagca gttcgcggtt ctcgagctgt tgatgcgggc cgacggcggg gtagtcagcg
      361 ccgagacgat gctggagaag gtctgggacg agaacgccga tccgttcagc agcgccccgc
      421 gggtgacgat gtcgaccctg cgcaaggccc tggggccgcc cgacctgatc gtcacggtga
      481 ccggggccgg ctaccgcctc ggcccgccgg aggatgccga tgccgactga acccgcgccc
      541 ccgattcgcc ctcgccggca gatgagcgcg cgactgcggc tgacgctgag ctacgccgtc
      601 ttcctcgtcg tcgccggctc ggtcgccctg ttcgggttct acatggcgct gcacctcggg
      661 ctcggtttca aactgcagtc cgccgacatc acctcgcggg gcgaactccg cgacgcgatg
      721 ctcgtcgcgg ccgccgcagc cctgctcggc ctcgcggcgg tcgggctcgg cggcggctgg
      781 ctgctggcgg ggtggatcct gcgcccgctc gacgacatca accaggccgc caggatcgcc
      841 gcgaccgggc gcctcgacca ccgcatccgg ctgaccggcc gcagcgacga gttcggccag
      901 ctcgccgaca acttcgacca catgctcgag cgcctccacg acgccttcct caaccaggag
      961 cggttcgccg ccaacgcctc acacgagctg cgcacgccgc tggcggtgac cgcgacgatg
     1021 ctcgacgtcg cccgcaccga tcccgaggga cgctacgacc cggcgctgct cgagcgcctg
     1081 cagctgacca acgaccgggc gatcgagctg accgaggcgc tcctgcgcct cagcgacgcc
     1141 aacgccgtca ccgccgtctc cgagccggtc gacctcgccg cactcgccgg cggtgcggtc
     1201 gaagagctgg aagccgaggc cgagcgccgc cacgtccagc tccacgcgaa gctggaggcg
     1261 gcgccgagcg tcggtgaccc ggcgctgctg cggcagctgg tcggaaacct ggccgagaac
     1321 gcggtccgtc acaaccgtcc cgaaggcgag gcatggctcg ccacgcacat cgacgccgtc
     1381 tcgtcccgcg cggtcgtgca ggtggagaac accggcccct cctacaccga ggagaccgcg
     1441 gcccagctgg tccagccctt cctccgcggc gccggccgca ccgccgccca gaacggggac
     1501 cgcggctacg gcctcgggct ggcgctggtc gcgcgcatcg tcaacgtcca cgacggcgag
     1561 ctgacgatct ccccccgccc gggcggcggg ctgatcgtca ccgtcgccct cccgccaccc
     1621 gcctaccggc ggtagaccag atgggtgacg tagggcgtgg tccggctcga ggccggcttc
     1681 agctgcaggt cgccgacacc gtcgaagagt cgctcgccgc ccccggcgac aaagggcgcc
     1741 acgtgtagcc gcagctcgtc gaccaggccc gcggcgaggt actggttgag ggtgccgggg
     1801 ccgccggcga tcgagacggt cttctcgccc gccgcctcgc gcgcccgctc catcgccgcc
     1861 tcgatcccgt cggtgacgaa gtggaagacg gtgccgccct ccatctcgat gctcgggcgc
     1921 gggcggtggc agaggacgaa ggtcggcgcg tggtagggcg ggttcggccc ccaataaccc
     1981 cgccagtcga gatcccactc accctcttcg gagctgaaca tcttgcgccc catgatgtag
     2041 gcgccggcca cggtgaccac ggcccgctcc tcttcgtttt cctcgatgtg atcgaactgc
     2101 cagctgtgca gtcggtcggc gtcgaggtcg ccgaacggat gctcgcgact ctggccccgg
     2161 ccgctcgcgt agccgtcgag cgtgatcgag atgtctgcgg ttacgtctgg cattgctctc
     2221 ctcctcggaa tgagtaacta gttgcaatct gcaagtggtg agcccacgct atcatcaacc
     2281 aattgcagaa tgcaagtaga aaacggaaga aggtgagcga cggccgctcg ggatgcccga
     2341 tcaacctctc gatcgaggtg atcggtgacc gctggtcgct gctcgtgatc cgcgacatga
     2401 tgttcggcaa ccgccaccac ttcggcgaat tgctggccgg atcggaggag gggatcgcct
     2461 cgaacatcct cgccgaccgc ctgcgccgcc tgaccgagca gggcctcgtc tcccgcgccc
     2521 ccgacccctc gcacaagcag aaggtgatct acaacctcac cgagccggcg atcgagctgg
     2581 tcccggtgat cgcccagctc ggcgcctggg gccgccgcca ccgcccggtc agccgcgagc
     2641 tctcgatccg tgccgagctg ctcgagcaag gcggtccgga gctgtgggag gaattcatgg
     2701 ccgagctgcg cgaacagcac ctcggcatcc cccgccccgc gggcgcggag tcggtcttcg
     2761 accgcctcca ggccgcctac gaggcggagc tggcaaaggc cggccagtga ttctttgatc
     2821 ttcggcgatg agttttgccg ccgctcaccg tctctaccta cagaccgttc agccggacaa
     2881 aggagagcac catgagcgcg acctacacct tcgacgtctt ttccagcctc gacggcttcg
     2941 gctccgccag cggcgactgg accggctact ggggcaagca gggccctgag ctgctcgacc
     3001 accgcctcgg cctgtacgac gaggagcagc gcatggtctt cggggcgacc acgtaccggg
     3061 ccttcgcgcg gatgctggcc gagagcaccg aggactccga ggtgcgcgac ccctgggtga
     3121 cgcggatgcg caacctgccg gcgacggtgg tctcgaccac gctggacggg ccgctcgact
     3181 ggccggacgc ctccctcgtc agcggcgacg ccgtcgacgt cgtcgcccgc ctgaaggagg
     3241 agtccgcggt ccccctgcgc tcgcacggca gcctctcgat gaaccgcgcc ctgatggccg
     3301 ccggcctggt cgaccgcgtc caggtgacgc tcttccccgt gatcaccggc cagaccggcg
     3361 aggagccgat cttcggcggc gcggccgact tcgacctcga gctgatcgaa acgcggacgc
     3421 tcgacggtga catccaggag ttggtctacc ggcccaccct gcactgagct gtggctcagt
     3481 gcagcagcgc cagcaccttc gtcaccttcc cgctggcgct ggggccgacc ggggcgacga
     3541 tcacccgcag cttctccgcc cggagtgcgg tcaggtaacg cttgcggctg agcttgaaga
     3601 cgatcggttt ccgctcaccg ccctcgatcg tgagggtctt ggagccgagc gccttcttcc
     3661 cgatccgcag cgtcacctgc atcccgcagg gaacggagcc ggtgcaaccg acggcgacgc
     3721 ggatccgccc cgtgccgctg agtcgcagct gcttggagaa gagggcgggc ttgccgaagc
     3781 cgagcacggc gggtggcggc gtcgacgggc cttccgtgat cggcttgtcg gggactttga
     3841 tctcgatcgg ccgcggcgtc gcggtcgggt cgtaggcgag cttcggggcg gctttggaga
     3901 ggacctggcc gtcgccgatc tgctgttcga gcgcgtacgc gtagccgagc agcttgccgt
     3961 cgtcccaggc gcggccggag aactggatgc cgttggggga accgctggcg tcgaggccgg
     4021 tcggcacatt caccgacggc aggttggaga ccgaggagac gttggagttc cagccgccgt
     4081 agccgttgag gctggtgccg gagatgccgc ccgcgtcgtc aagcggggcg gtccgcccgg
     4141 gcgagatcgt cgcatcgacg tcgctggcgt ccatccagtc catgatttcc tggcccagcg
     4201 cccagcgtcc tttgacgagt ttttcctcgt ccgcggtggt caggatcggt ccgtcccatt
     4261 ccgtcttcca ggcgttgatc ggcgagtgca gcggcgaggt gaacatctct ttgccgttcg
     4321 cgaacccggt gccgggatgt tcctccagcc aacggccgta ggcctccacg acccaggcga
     4381 tcacgtcgac cccgccggtc cagtcgctgg ccggcagggg cgtcggcatc tcgatgattt
     4441 cggcgccggc cgcttcgagg tcgtcgaact gttcttcggc gagcgcgagc gagctggtct
     4501 cgccgtcgag gttcgtgaag gcagccgggt cgtagccgag ccgcacgccc tggagcgcgt
     4561 ctttcgagag gaagctccgg tagtcggcgg ctttgcgcgc atccgattcc gccgtctcgg
     4621 ggtcgttcgg gttggtgcca gaggtgacgt tgaggatcg
//
